LOCUS       DAELJO010000001        77947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELJO010000001 DAELJO010000000
VERSION     DAELJO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.001"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            28..678
                     /locus_tag="KPB88_000001"
     CDS             28..678
                     /locus_tag="KPB88_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0052318.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            692..1252
                     /locus_tag="KPB88_000002"
     CDS             692..1252
                     /locus_tag="KPB88_000002"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG0052319.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            1269..4655
                     /gene="mfd"
                     /locus_tag="KPB88_000003"
     CDS             1269..4655
                     /gene="mfd"
                     /locus_tag="KPB88_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG0052320.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            4686..5681
                     /locus_tag="KPB88_000004"
     CDS             4686..5681
                     /locus_tag="KPB88_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0052321.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            5805..6344
                     /gene="spoVT"
                     /locus_tag="KPB88_000005"
     CDS             5805..6344
                     /gene="spoVT"
                     /locus_tag="KPB88_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG0052322.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            6464..8077
                     /locus_tag="KPB88_000006"
     CDS             6464..8077
                     /locus_tag="KPB88_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0052323.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAIINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            8184..9632
                     /gene="mazG"
                     /locus_tag="KPB88_000007"
     CDS             8184..9632
                     /gene="mazG"
                     /locus_tag="KPB88_000007"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG0052324.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            9721..9999
                     /locus_tag="KPB88_000008"
     CDS             9721..9999
                     /locus_tag="KPB88_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG0052325.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            10071..10313
                     /locus_tag="KPB88_000009"
     CDS             10071..10313
                     /locus_tag="KPB88_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG0052326.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            10375..10629
                     /locus_tag="KPB88_000010"
     CDS             10375..10629
                     /locus_tag="KPB88_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG0052327.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            10630..11163
                     /locus_tag="KPB88_000011"
     CDS             10630..11163
                     /locus_tag="KPB88_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052328.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            11169..11453
                     /locus_tag="KPB88_000012"
     CDS             11169..11453
                     /locus_tag="KPB88_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG0052329.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            11566..12486
                     /locus_tag="KPB88_000013"
     CDS             11566..12486
                     /locus_tag="KPB88_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG0052330.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            12576..14948
                     /gene="spoIIE"
                     /locus_tag="KPB88_000014"
     CDS             12576..14948
                     /gene="spoIIE"
                     /locus_tag="KPB88_000014"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG0052331.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            15299..17191
                     /gene="pepF"
                     /locus_tag="KPB88_000015"
     CDS             15299..17191
                     /gene="pepF"
                     /locus_tag="KPB88_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0052332.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            17305..18270
                     /gene="galU"
                     /locus_tag="KPB88_000016"
     CDS             17305..18270
                     /gene="galU"
                     /locus_tag="KPB88_000016"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0052333.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            18770..20374
                     /gene="rho"
                     /locus_tag="KPB88_000017"
     CDS             18770..20374
                     /gene="rho"
                     /locus_tag="KPB88_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG0052334.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            20527..20727
                     /gene="rpmE"
                     /locus_tag="KPB88_000018"
     CDS             20527..20727
                     /gene="rpmE"
                     /locus_tag="KPB88_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG0052335.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            20872..21765
                     /locus_tag="KPB88_000019"
     CDS             20872..21765
                     /locus_tag="KPB88_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG0052336.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            21783..22631
                     /gene="prmC"
                     /locus_tag="KPB88_000020"
     CDS             21783..22631
                     /gene="prmC"
                     /locus_tag="KPB88_000020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG0052337.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            22675..23739
                     /gene="prfA"
                     /locus_tag="KPB88_000021"
     CDS             22675..23739
                     /gene="prfA"
                     /locus_tag="KPB88_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG0052338.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            23868..24548
                     /locus_tag="KPB88_000022"
     CDS             23868..24548
                     /locus_tag="KPB88_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG0052339.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            24577..25617
                     /locus_tag="KPB88_000023"
     CDS             24577..25617
                     /locus_tag="KPB88_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG0052340.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVASKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            25634..26083
                     /locus_tag="KPB88_000024"
     CDS             25634..26083
                     /locus_tag="KPB88_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG0052341.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            26100..26552
                     /gene="rpiB"
                     /locus_tag="KPB88_000025"
     CDS             26100..26552
                     /gene="rpiB"
                     /locus_tag="KPB88_000025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0052342.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            26588..27217
                     /gene="upp"
                     /locus_tag="KPB88_000026"
     CDS             26588..27217
                     /gene="upp"
                     /locus_tag="KPB88_000026"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG0052343.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            27518..28333
                     /locus_tag="KPB88_000027"
     CDS             27518..28333
                     /locus_tag="KPB88_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG0052344.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            28559..28996
                     /locus_tag="KPB88_000028"
     CDS             28559..28996
                     /locus_tag="KPB88_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0052345.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            29181..29408
                     /locus_tag="KPB88_000029"
     CDS             29181..29408
                     /locus_tag="KPB88_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG0052346.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            29413..29799
                     /locus_tag="KPB88_000030"
     CDS             29413..29799
                     /locus_tag="KPB88_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG0052347.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            29802..30506
                     /gene="atpB"
                     /locus_tag="KPB88_000031"
     CDS             29802..30506
                     /gene="atpB"
                     /locus_tag="KPB88_000031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG0052348.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            30575..30835
                     /gene="atpE"
                     /locus_tag="KPB88_000032"
     CDS             30575..30835
                     /gene="atpE"
                     /locus_tag="KPB88_000032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG0052349.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            30958..31455
                     /gene="atpF"
                     /locus_tag="KPB88_000033"
     CDS             30958..31455
                     /gene="atpF"
                     /locus_tag="KPB88_000033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG0052350.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            31452..31997
                     /locus_tag="KPB88_000034"
     CDS             31452..31997
                     /locus_tag="KPB88_000034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG0052351.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            32014..33516
                     /locus_tag="KPB88_000035"
     CDS             32014..33516
                     /locus_tag="KPB88_000035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG0052352.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            33544..34422
                     /gene="atpG"
                     /locus_tag="KPB88_000036"
     CDS             33544..34422
                     /gene="atpG"
                     /locus_tag="KPB88_000036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG0052353.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            34435..35829
                     /gene="atpD"
                     /locus_tag="KPB88_000037"
     CDS             34435..35829
                     /gene="atpD"
                     /locus_tag="KPB88_000037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG0052354.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            35832..36092
                     /gene="atpC"
                     /locus_tag="KPB88_000038"
     CDS             35832..36092
                     /gene="atpC"
                     /locus_tag="KPB88_000038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG0052355.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            36554..36934
                     /locus_tag="KPB88_000039"
     CDS             36554..36934
                     /locus_tag="KPB88_000039"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG0052356.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            36949..37410
                     /locus_tag="KPB88_000040"
     CDS             36949..37410
                     /locus_tag="KPB88_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0052357.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            37431..38021
                     /locus_tag="KPB88_000041"
     CDS             37431..38021
                     /locus_tag="KPB88_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052358.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            38036..39193
                     /gene="alr"
                     /locus_tag="KPB88_000042"
     CDS             38036..39193
                     /gene="alr"
                     /locus_tag="KPB88_000042"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0052359.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAVKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            39226..39513
                     /locus_tag="KPB88_000043"
     CDS             39226..39513
                     /locus_tag="KPB88_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG0052360.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            39482..39865
                     /locus_tag="KPB88_000044"
     CDS             39482..39865
                     /locus_tag="KPB88_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG0052361.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            40036..40863
                     /locus_tag="KPB88_000045"
     CDS             40036..40863
                     /locus_tag="KPB88_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0052362.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEEMNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGVDNE"
     gene            40856..41797
                     /locus_tag="KPB88_000046"
     CDS             40856..41797
                     /locus_tag="KPB88_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0052363.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKTF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            42220..43314
                     /locus_tag="KPB88_000047"
     CDS             42220..43314
                     /locus_tag="KPB88_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052364.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            43539..43955
                     /locus_tag="KPB88_000048"
     CDS             43539..43955
                     /locus_tag="KPB88_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052365.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(44137..44706)
                     /locus_tag="KPB88_000049"
     CDS             complement(44137..44706)
                     /locus_tag="KPB88_000049"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG0052366.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGEYGIR
                     EPNEKSPTVDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            44860..46017
                     /locus_tag="KPB88_000050"
     CDS             44860..46017
                     /locus_tag="KPB88_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG0052367.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(46584..47777)
                     /locus_tag="KPB88_000051"
     CDS             complement(46584..47777)
                     /locus_tag="KPB88_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0052368.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(47857..48060)
                     /locus_tag="KPB88_000052"
     CDS             complement(47857..48060)
                     /locus_tag="KPB88_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBG0052369.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            complement(48479..48736)
                     /locus_tag="KPB88_000053"
     CDS             complement(48479..48736)
                     /locus_tag="KPB88_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052370.1"
                     /translation="MNGLNGNQNDYPENALVPYPVILAASKGDPDAMKIVLQHFSGYI
                     ASLSMRKLYDERGNVYFGVDEEIRERLQAKLMRAILTFRAE"
     gene            complement(48717..49157)
                     /locus_tag="KPB88_000054"
     CDS             complement(48717..49157)
                     /locus_tag="KPB88_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0052371.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVAFS
                     DLALHEERQLYTVDKYFQDEEVEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNLSRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            complement(49620..50375)
                     /locus_tag="KPB88_000055"
     CDS             complement(49620..50375)
                     /locus_tag="KPB88_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052372.1"
                     /translation="MKVLIKNEFTKLKREWFVLFLLLLSWLPVLTGGVGAVFNNSTKT
                     VSDLFFFMNNQFSMFFPMVIVILTGSLFYQEYKNKTYINWITYGYSKQQLFSAKIIVG
                     FVIEIVFAASLICLFHILILMLKIAGKVSVDIQEILSLTVGFALEAIIVILITTCIAA
                     IIINISRNIIVTSVIGVIYGFVSCFFIGSEFGYVIPGSFAYRLSMYFSDSSTYYELAK
                     QATIGGCISSIISFGLMLFMGIFIFTRKKKIES"
     gene            complement(50372..51064)
                     /locus_tag="KPB88_000056"
     CDS             complement(50372..51064)
                     /locus_tag="KPB88_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0052373.1"
                     /translation="MLQIKNVNLCVGSNKLLHNINLSLEQGKIYGLLGANGAGKTTLF
                     KSMLGLTAYTGEILSDKNPAKSSEFGSLIEYPAFYPKLSVMENLKLHASYIGLTNPDL
                     ENSLKQVNLWEARNKLFSQLSLGMRQRLGIARTFLGNAKYLLLDEPTNGLDPMGIKDI
                     RILLRDKLKTPKHCIIVSSHNLTEIAAIADILIFIRNGQIIEMIENKYDEKELEVLYE
                     NLMTSITTEEVQ"
     gene            complement(51077..51253)
                     /locus_tag="KPB88_000057"
     CDS             complement(51077..51253)
                     /locus_tag="KPB88_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="receptor"
                     /protein_id="HBG0052374.1"
                     /translation="MLSKISLIISIVALAVCFIAAISGILSDGMKGLQKKGKLLGVTF
                     CIYAITFLTFIISQ"
     gene            complement(51363..52259)
                     /locus_tag="KPB88_000058"
     CDS             complement(51363..52259)
                     /locus_tag="KPB88_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0052375.1"
                     /translation="MSIFCIFLVVTSLILLLKVIIQKQQIAKIKNQIDFLCSRDTQAE
                     ITLEKLDKQTEELAISINHLLKKYRLTGIKIERNDSLFRDTITSLSHDLRTPLATANG
                     YIQLLLEQPLSAEQKEYVKIANERVSAVKILLDQLFEFARIEANELKLNKQLVDANSV
                     LRDALATYYNDFEIKAITPTIDIPDSPAMIWADKDALFRVFSNIIYNALIHGDSVYTI
                     HATVERKCYYISISNHSNTICKEDIPHLFDRFYTTDSSRTKKTTGLGLAIARKLITQM
                     GGEISAHLHNEIFEICISFTIL"
     gene            complement(52261..53037)
                     /locus_tag="KPB88_000059"
     CDS             complement(52261..53037)
                     /locus_tag="KPB88_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052376.1"
                     /translation="MIKMIQGELFRIRKNRLFKICLIVSTFFVFALALTFHYNTIKHS
                     VAAVPKASLNDYTQFLFSDYSLILPITVFLGCYFTENSRTGTCSIFLSKGITRTSIFF
                     GKLISSWIATVLYMLFNFLMAYIFILVMWVKQSYIDCSFGLIGIYLFLQILCFIGYTS
                     FIWLLSILFRYRSLVITISIFLLGTLYFYLTKISSALDLSYSLYRYWIAGLSHAMEIR
                     LVLPQLPLILLTITGYVFFPTLCSFVIYKKIDLRKHERDK"
     gene            complement(53041..53967)
                     /locus_tag="KPB88_000060"
     CDS             complement(53041..53967)
                     /locus_tag="KPB88_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052377.1"
                     /translation="MHNTYTIIERGGSVGLVLEVKNLSYVFPNGVGIIGIDFQIDYND
                     IYYLYGSHNSGKSLILQSILGIISPQKGSIKIFENPDFLQEKRRIGYVPQSPYYIDRM
                     TITDMMNYFSLSFGVLEKNFADILNLNLKEKKPVRHLSSFIQRKLNLWIALLGNPDLV
                     FIDDPFGGLDTQESEQLISIISYLNKNNNITFLLTGHNYELASRIATKYGVLKNGRFL
                     IELTPEKLDSECKRCIKIRTPQLLSAIPLLEKEFPQYEVLEDDLIRVFCPLSHSAKIN
                     TLLVSAGIEVSEIWIAGTMPQEYLLKLTEGGI"
     gene            complement(53999..54688)
                     /locus_tag="KPB88_000061"
     CDS             complement(53999..54688)
                     /locus_tag="KPB88_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0052378.1"
                     /translation="MQQILQILIVEDDILLSDMLKSLLSQNGYRVNTAYSGSEALLIL
                     EKETFDLILLDLMLPGISGEEVLTTIRKNQTTPIIGVSAKTDIDSRIDLIKNGADDYI
                     TKPFDNKELLVRMEAILRRCHRSSNNSLLKFKDLMLNPATMEVTIKDIPLVLTRYEYL
                     ILQLLMTAPNKVFTKNNIFESVWNETFCGDDNAINVHISNLRKKFAKINPNEQYIQTV
                     WGLGFKMQSNP"
     gene            complement(54657..54851)
                     /locus_tag="KPB88_000062"
     CDS             complement(54657..54851)
                     /locus_tag="KPB88_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0052379.1"
                     /translation="MEYISCMEAAENWGISERRVQKLCEENRIPGATKFSRIWLIPKK
                     AEKPIDRRKQHAADTTNSNR"
     gene            55027..55386
                     /locus_tag="KPB88_000063"
     CDS             55027..55386
                     /locus_tag="KPB88_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052380.1"
                     /translation="MKQSKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYIAS
                     IENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLKIM
                     SATAKGIEEANNDRTGE"
     gene            55630..55959
                     /gene="mobC"
                     /locus_tag="KPB88_000064"
     CDS             55630..55959
                     /gene="mobC"
                     /locus_tag="KPB88_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG0052381.1"
                     /translation="MNGRKRKIQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIARRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            55920..57263
                     /locus_tag="KPB88_000065"
     CDS             55920..57263
                     /locus_tag="KPB88_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG0052382.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YVEYQAEKVGTSWKGKLKIAIDTLIPQVASFEELLQRLQAAGYEIKTGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIEGKRTKAAKTPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIEEINAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAAKALKASGITKLPNLA
                     AMQEEYGKLQAQKEALYADYGKLKKQVKEYDVIKQNIDSILRQGKEQERGKETER"
     gene            57335..57958
                     /locus_tag="KPB88_000066"
     CDS             57335..57958
                     /locus_tag="KPB88_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG0052383.1"
                     /translation="MKSILLHGLGQTSSDWDKTIEVMENRMDIICPNLSEWLVDKEPC
                     YNTLYQMLENYCNQFNEPLNLCGLSLGGILAMQYGIEHTDRVNSLVLIGTQYTMPKRL
                     LRFQNMLFHIMPKSVFSQIGFPKKDFISICSSMIELDFQQSLRDIQCPVLVLCGSKDN
                     ANKTASVDMEKMIPCAKLSIIQNAGHEVNKDNPVELGKQLNTFFNLK"
     gene            complement(58129..58494)
                     /locus_tag="KPB88_000067"
     CDS             complement(58129..58494)
                     /locus_tag="KPB88_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG0052384.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGAAFASTSNSVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(58491..58697)
                     /locus_tag="KPB88_000068"
     CDS             complement(58491..58697)
                     /locus_tag="KPB88_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG0052385.1"
                     /translation="MIRLTVEETNLLSIYNEGGKRGLTENINAALPFMDEDMRELAKR
                     TLAKIAPLTESEYVELAIFAADEV"
     gene            complement(58700..62785)
                     /locus_tag="KPB88_000069"
     CDS             complement(58700..62785)
                     /locus_tag="KPB88_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0052386.1"
                     /translation="MASLRDTVKEYQEELRDGIAWVAFWKTGRSWNAEDFHLELGEYL
                     YPEDRSRMEEIKQADPAAIVVNGYYSGYLGEDRNLDELTAGVRRHYENGYSNIREFIE
                     AHDDRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIE
                     KERSERMEETILSGYLSNLGKYTEGRPMGEWVSFPTTAEHLKEVFDRIGIDGKHYGEL
                     HITEYQSSIPGLAEKLTELESLDELNYLGELLKMQFDDDREKFAAAITYGDHTRDLQD
                     IINLAQNLDCYWIYPSVKTEEDYGHYLIEELDELELPEEAKNYFMYEEYGRDAAINDG
                     GRFTEQGYIYNNRNTFTQWYNGRDVPEEYRVIPQLPVQEKEQADLDASAAIQTAATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILELYESDRYADYLRTMSKFHDYSLNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEKEIKIPAFRVVSVFDVSQTEGKELPALTYELTGNVEQYKDFFAALEKTSPF
                     AIGFEALNDGIKGRCNYEEKRIFINEGMDELQNIKTAIHEIAHATLHDIDKDAPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAASLI
                     DTIDGHFAEIQKAQDKEQTTEQTQPTQETAKQPEAEVAAPELPEETAPVQEKAAQTEP
                     EADTGASSETPQTTQPEQAAPVAPYYTINEAAARRAKNANSFSDYKQGSATAEYRHYV
                     DEAVQIAERQKQRVDPMYHEKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNF
                     PTRKKEKQNAARDSNYREWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKS
                     QETMKAVNAYYRKHKTLDGCPHLPPEELEKLKADMASSWHLEDKPFATWALSNNSAEI
                     RRVKDRIKSLSQQKEIGFVGWEFDGGKVEANTEANRLQIFFEDKPDEATREALKSNGF
                     RWSPKAGAWQRQLTSNAYYAADYVKSIQPLTGEKPTELQRAHIRQQKVAAQEQPVQEQ
                     PEQKAPQDKDTFSIYQIKGGDETRDLRFEPYDRLTATGHRVDPKNYALVYSAELTPGT
                     SLEDIYTRFNIDHPKDFKGHSLSVSDVVVLHQNGQDTAHYVDSFGYKEVPEFFKEQEK
                     ALIPDDLETGETIKTPRGTFYVTAMSREQMEAAGYGLHHQSDDGKYLIMGNGTRAFAI
                     FAEQPENYLKAAEQTTEQNYNMIDGQINNTPTTAELEAKVKAGETISLVDLAEAVKAD
                     KERGKAAKTEKKPSIRAQLRADKEKTAQKKAAKSKNHELEV"
     gene            complement(63002..65089)
                     /locus_tag="KPB88_000070"
     CDS             complement(63002..65089)
                     /locus_tag="KPB88_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG0052387.1"
                     /translation="MLVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLVG
                     LAEAAAYGEQYKKWSYDSLPILPQEWKYTVASDKEKQFKTLKELMHRADVSEVVNACD
                     AGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALCR
                     AKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLTL
                     SGAEAASERISDKAEADALKTTCEASRAVCTSLVKEKKTTAPPKPFDLTSLQREANRL
                     FGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFSFMAGKDF
                     TPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGARLIMATAAPHTF
                     EAVTAVFECAGQSFTARGKTILCEGWKEIDRRYRAALKNKPETDDANSDTENTLPPFA
                     DGQTFENPAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAAV
                     IEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADPA
                     AFMEGIENMARELVKTYPFLSDDKAQMFKPEREVLGNCPRCGSPVYEGKKNYYCSNKD
                     CIFTMWKNDRFFEERKVTFTPKIAAALLQSGKVNVKKLYSPKTGKTYDGTIVLADTGG
                     KYVNYRVELPKKK"
     gene            complement(65117..65305)
                     /locus_tag="KPB88_000071"
     CDS             complement(65117..65305)
                     /locus_tag="KPB88_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052388.1"
                     /translation="MTLFTDNPFEKMMIQKPDGRRDNAPPVPYPPACASCPYKGQSPC
                     VGYCLKQVQKKKETEPER"
     gene            complement(65302..66030)
                     /locus_tag="KPB88_000072"
     CDS             complement(65302..66030)
                     /locus_tag="KPB88_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG0052389.1"
                     /translation="MKNKLKKTMTALCAALILMGGFSVPAYAQGTAAEPPADNATNDS
                     NVVVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPEICNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFLKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETSEDEETEEETL"
     gene            complement(66020..66289)
                     /locus_tag="KPB88_000073"
     CDS             complement(66020..66289)
                     /locus_tag="KPB88_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG0052390.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATETADYEEQEENTDEE"
     gene            complement(66321..68237)
                     /locus_tag="KPB88_000074"
     CDS             complement(66321..68237)
                     /locus_tag="KPB88_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG0052391.1"
                     /translation="MTRDGAVEINETQQTAERISSREADSDFSQPDNAAAERVMEHLD
                     AAHTRKANKKAVKKEQEATALRTSTSRLQFTDEERTTPELEPYIKKSEKAADKLDEAK
                     AAIPKQKKLVKERTFEEATGKAKTRLYFEEQEKPIPGGKAHRNPLSRPAQEAGIFVHN
                     KIHSVEKDNSGLEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKA
                     NVNFQYHKTLHDNPQLTSNPLSRFMQKQQIKRQYAKSAKKGGAKAAAEATRKTAKKTA
                     EETRKAAAFAARHPAGILIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDS
                     DLVATEQSYAAMENELQSEIDAIESTHSGYDEYRYDLDTIGHNPHELASYLTALLQSY
                     IPQSAQAELKRLFGLQYTLTLTEEIEIRTTTDEEGNEEEYEYRILNVKLTNKPIASLA
                     EELLNPQQFEMFKVYLQTQGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRPGNQELMEL
                     AKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQGVPWFQSR
                     GQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDESRVYTVEGNSGDACKIKSYD
                     LNYQCIKGYGLMNW"
     gene            complement(68273..68569)
                     /locus_tag="KPB88_000075"
                     /pseudo
     CDS             complement(68273..68569)
                     /locus_tag="KPB88_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
     gene            complement(68872..71058)
                     /locus_tag="KPB88_000076"
     CDS             complement(68872..71058)
                     /locus_tag="KPB88_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBG0052392.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKVAQRKYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKTQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDVLNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEILAPELNDRILADMLD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSVPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFSLVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRDYLADPDPAKMPILGDLYDELLKQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGKQAQSLQSCKWGLCMPS"
     gene            complement(71006..71410)
                     /locus_tag="KPB88_000077"
     CDS             complement(71006..71410)
                     /locus_tag="KPB88_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG0052393.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSVALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGSRRKQA"
     gene            complement(71432..72301)
                     /locus_tag="KPB88_000078"
     CDS             complement(71432..72301)
                     /locus_tag="KPB88_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052394.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNTNIFNMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            complement(72320..72535)
                     /locus_tag="KPB88_000079"
     CDS             complement(72320..72535)
                     /locus_tag="KPB88_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG0052395.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(72735..72902)
                     /locus_tag="KPB88_000080"
     CDS             complement(72735..72902)
                     /locus_tag="KPB88_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052396.1"
                     /translation="MNKRIRKKKDKKITKAINGLVLIAERREQQRQAAIRRFVKRCEA
                     LYEQQRTERRN"
     gene            complement(72895..74685)
                     /locus_tag="KPB88_000081"
     CDS             complement(72895..74685)
                     /locus_tag="KPB88_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG0052397.1"
                     /translation="MNAINWKKLLLPNIPYLLFVYLFDKVGQAVRLAPGADFSGKVLS
                     LADGFSAAFANPFPSLAPMDLLIGIAGAVLIRLIVYVKGKNAKKYRKGIEYGSARWGN
                     AEDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMRYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            complement(74682..75164)
                     /locus_tag="KPB88_000082"
     CDS             complement(74682..75164)
                     /locus_tag="KPB88_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG0052398.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDRQKI
                     PHGKQTLKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTETPPRYLVFF
                     KGRDADALTAAFKEFSAKKLTQEQKPSIRKLLSTLKDKAAALNAQRDKVKNKDRGIAR
                     "
     gene            complement(75258..75476)
                     /locus_tag="KPB88_000083"
     CDS             complement(75258..75476)
                     /locus_tag="KPB88_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG0052399.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            complement(75501..76454)
                     /locus_tag="KPB88_000084"
     CDS             complement(75501..76454)
                     /locus_tag="KPB88_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052400.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINREKIDAIREAVRELENAGYIQRSRERDEKGRLRGTTYVIYEQPPKLDL
                     PTLEKPTLDNPTLEKPMLENPTQLNKEIQKTNLPTKEKLNTDISSIHSIPFHSLNPSP
                     LEDAAQPPERKRKEATDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILALILETVC
                     TKRRTIRIAGDDHPAELVKAKFMKLNSEHIRFVLDCMQENTTKIRNIKQYMKAALFNA
                     PSTIGSYYTSLVSHDMYDGRTIPSARSKGIPDYTCNEGESL"
     gene            77068..77550
                     /locus_tag="KPB88_000085"
     CDS             77068..77550
                     /locus_tag="KPB88_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0052401.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT        26457 a        12138 c        15651 g        23701 t
ORIGIN      
        1 aaccctatta ctaaagtgct acaaactatg gagtatataa taataatttc cttatttttt
       61 gcacatttga taaataaatc tctatgtgtt ttttgtgata gagaaaagaa aaatttatat
      121 aatacgtttt ttatttacat aataactgta gttttatctg tattaatata tctaaaattt
      181 gggattagtg tagaatgtgt acaatatatg gttttaattc catttttaat aattatatcc
      241 attgtagatt actacacaat atatgtatat gatataacaa tagttagtgg tataatagtt
      301 caaggtataa tttttttggc ctttttcaat atagctcaat ttgattatat aaatcatata
      361 tttggactat tgataggctt tattatacct tatattattg ctaaactaac aaaaggatta
      421 ggctatggag atgtaggtct ctatagctta tgttgctttg cattgggaaa taattatggg
      481 ttttatgtga tagttttatc atttatttta gcgtttttgt tttttattat tttatacata
      541 actaaaatat ttaaagatgt agatataaga aagcaaattc cttttacacc ttttatttcc
      601 ttgtcaacaa ttttaataat atttacagag tataatatat ttaattttta ttataatttg
      661 ttatacaaat ttatataaca ggaggtaaga aatgtacgta gtagtagggt taggtaatcc
      721 aggaaagaag tatgagaaga ctagacataa cgttggattt gatgttatag atattcttgc
      781 taaagaatac aatattagtg ttactaaaat aaagcataaa gctttgattg gtgaagggag
      841 agtaggtact gaaaaagtat tattagttaa accacaaact tatatgaatt taagtggaga
      901 aacattaata gatatatata aatattataa ggttgattta agtaatatag tagtagtata
      961 tgatgatata gatttagaag ttggaaagat acgtataaga aaaaaaggaa gtggaggaac
     1021 tcataatgga atgaaatcta taactaagtg cctaggttca aatgactttc caagagtgag
     1081 agtaggtgta tctaaaccag aagctggtca agacttagca gactttgtac tttctagatt
     1141 tagaaaagaa gaatctgata acataaatga agcattagaa aaagcggcag atgctattga
     1201 ctcaataata agagaaaata tagatatgtc tatgaacaaa tataatggat agtgagaggg
     1261 agatttaaat gaatgatgtt tttttatatc ctttgcaaaa ttcaaaagag tataaagata
     1321 taataaactg tataaaaaat acaaaaggct cattattggt taatggactt ttaccagtac
     1381 aaaaacctca tatatcgtat tctatattta atgatttatc acgacaaatg atttttataa
     1441 cgagtagtga tttagaagca aagaaagtat acgaagattt aagtttttat atggaagata
     1501 aagttgaata tttaggtttt caagatattt atttttatca cttggatgca aaggacagaa
     1561 acgaagaagc aaagaaatta aaagtattgt taaaactagc aaataaagaa aaaatcattt
     1621 tagtaacttc tattgaggca gttttaagaa aatacatacc aaaacaagtt ttgctcgata
     1681 gtgtatctca ttataaggtg ggagatagct tagacttaga aaagttaact gagaaattag
     1741 tttcattggg atatgaaaga gtttcaaaaa tagaggggtt tggtcaattt agtataagag
     1801 gtggaattat tgatgtattt tcacttgaat atacaaatcc aattcgtatg gaattatttg
     1861 atgatgagat agattccata cgaacttttg atgtatattc acaaaaatca attgataaat
     1921 tgcaacaatt ctctataact ccatctagag aatttattta tcccgaaaaa actacagatg
     1981 cattagttaa attaaaaaaa gaaacaacta aaaatactga tgaaggtaca tttcaaaata
     2041 tagattatat atcaagtaag acttattttg aaggtgcaga gaactatata gattatattt
     2101 atccagaaga aaacaaaagt atatttactt atttagcaga tgatgcaata gtatttatca
     2161 atgatataac gagacttaaa gaaagatgcg aaaattatat aaatgagttt agagaaaatt
     2221 ataaattaaa tcttgaaaga ggtctggcta ttaaaaacca aggtaaattg ttatatcact
     2281 atacagattt agagtactta gtaaaagaca agtctgtggt attaaactcg ctattgccaa
     2341 agtctataaa taacttttct ataaaatcta taataaattt tgaaagtaga gaagttccta
     2401 catttaatgg aaaggtagat ttgcttgtag aagaattaaa tcggctgaaa tataatggat
     2461 acaaaataat tttagcaaca aataccttag aaagagcaaa taaattagga aaagatttat
     2521 tggacaaagg cttagaaacg accatttcta aagatagaga tattgaaatt aaatcttcac
     2581 aagttataat tgtacctgca cacataaaca gtggttttca gtataaatct ataaagtttg
     2641 ttgttataac agataacgaa atgataggtg tttataaaag agcgtctaag actagcaata
     2701 aaaaagttaa aaagggaaag aaaatagaat cctttttaga tttaagtgtt ggagactatg
     2761 tagtccatga aaatagtggt gtaggtagat acacaggaat tgaacaaatt acagtcaatg
     2821 ctattaaaaa agattatatg aaaatagtat atcaaggtgg agataatcta tatgtgccaa
     2881 tagaccagat ggacaaagta caaaaatata ttggtgcaga agtagaaaaa gttaagttaa
     2941 ataagcttgg aacaaatgag tggacgaaag caaaagcaaa agttaaaaaa gaaattgaag
     3001 atatgacaaa agacttgatt gagctttatg caaaacgtga aaaaatacaa gggtataagt
     3061 tttcaaaaga tacaccttgg caggcagagt ttgaatcgtt gtttccatat caagaaacag
     3121 aagaccaatt aaaggctata gaagaaacta aaaaagatat ggaatcaagt aaggtaatgg
     3181 ataggctagt atgtggagat gttggttatg gaaaaacaga ggtagctatt agaagtattt
     3241 ttaaagcttg tatggaccaa aaacaagttg ctgtattggt tccaacaaca atattggccc
     3301 aacaacatta taatacattc aaagagaggt ttgaaaatta tcctttgaga gtagaagttt
     3361 taagtaggtt taaaacacca aaacaacaaa aacagattat tgaagatgct aaaaaaggat
     3421 tagtagatat tttaattgga acacatagaa taatatccaa agatataaat ttaccaaact
     3481 taggtcttgt agtcattgat gaagaacaaa gatttggagt aaaacataaa gaagctttaa
     3541 agaaaattaa atctacagta gatgtactta cactttcagc aactcctata ccaagaacat
     3601 tacatatgtc tttaagtgga ataagagaca tgagtgttat agaagaacct ccacaagaaa
     3661 gacatcctgt aataacatat gttacagaga gtaaggaaag cgtaattcaa gatgagatag
     3721 agagagaatt aagtagaggt ggacaggtat tttttgtata caatagagtt gaacacattg
     3781 aagaaatggc aagtatgatt caaaaactcg taccagatgc tagagtggct gttgcacatg
     3841 gaagaatgac ttctaaatca ttagaaaata taatcctagg atttttaaat aaagattatg
     3901 atgtactagt ctgtactaca ataattgaaa ctggtatgga catatcaaat gcaaatacaa
     3961 tgattatata tgatgcagat aaaatgggat tggctcaact ttaccaattg agaggtaggg
     4021 ttggaagaag ttcaagacaa ggatatgcat atcttttgta tgaaaaagat aaaactttaa
     4081 gtgagatagc ggagaagaga ttaaaagcta tcagagaatt tacagagttt ggttctggtt
     4141 ttaaaatagc aatgagagac cttgaaataa gaggagcagg aaatatttta ggttctcaac
     4201 aacatggtca tatggcagtt ataggttatg atttgtacgt taaaatgctt aatgatgcaa
     4261 ttaaaaaagt caaaggtgaa cctattgtgg aggaaattga tgttgaaata gatttaagtg
     4321 taaatgcata cattccagat aattatatca aagatgagtt gattaaaatt gaaatgtata
     4381 agaaaatagc ttctattgaa aacaaagaag acatgttgga tatacaagaa gaacttgagg
     4441 atagattctc tgatatacct aaaccagtcc agacacttct tacaatagct tatataaaat
     4501 cgttatgtaa gatattaaaa atagaaaaga taagacaatt aaaagatgaa atattattag
     4561 ttccaataac aaagtataga actaaacaga aaataggtta taatatagtt acagaattag
     4621 aagaattatt agaaaagatg tgcaaggtaa aataacttgt aaatttaaga agggatgttt
     4681 taaatttgaa aaaagtaata acattggtaa tagctatgat tttggtagta tctgtgactg
     4741 cttgcagttc tagtaaagga gaaactgtgg caacagttga aggtacaaag atatcatcag
     4801 atgaatttaa aaaaactata gctctttata aagattctat ggaacaaact tatggaaaag
     4861 atatatggga taaggaagtt gaaaagggtg taaaatacaa ggacaagttt aaagatttaa
     4921 ttcttgacca acttataact acagaagtaa tatattctca agctaaaaaa gataacttac
     4981 ttcctaaaaa agaggatgtt gaaaaaagtt ttaaagaatt aaaagacgct atgggtaaag
     5041 atgaaaaata taaagaacaa cttaagaaat taggtataga tgatgagttt ttaagagacc
     5101 aacaagagaa agatttagca atgcaaaact atcaaagtaa ctttgctaaa aagactaaaa
     5161 tatcagatga agaaatgaaa aaatactatg atacacataa agatgaattt aaaaaagatg
     5221 aggtagaagc ttctcatatt ttattgaaaa cagtagatga taataacaag ccactttcag
     5281 ataaggaaaa agctgaagct aagaaaaagg ctgaagaggc tcttaaagaa gttaaatcag
     5341 gtgaggattt tgcaaaagta gcaaaaaaat attcacaaga tacttcagca agtgatggtg
     5401 gtaaattagg attcttttca aggggtcaaa tggttgctga atttgaagat gctgcttttt
     5461 ctatgaaaaa gggtgaagta tctgatttag ttgaaactca atatggatac cacataatta
     5521 aagtgacaga cagaataaat gagcaaacat catttgaaga tgctaaagaa actataaaag
     5581 accaactgtt gaaaaataaa tatcaagaac aaatagaaaa attaactaaa gaagctaagg
     5641 tagaaaaaga tgaaaaagta ataaataaaa tcacaatcta agacttaaaa aaagagtaat
     5701 atgcttaaaa aagctatatc tggataagat atagcttttt ttgtatattt ttcttaacaa
     5761 tggtaaaaat aaacttaaag taatttatgg agggattaaa aaatatgaaa gcaacaggta
     5821 tagttagaag aatagatgat cttggaaggg tagttatccc aaaagagata agaaagacat
     5881 taagaattag agaaggagac cctttagaga tattcacagc aaaagatgga gaagtcatac
     5941 ttaaaaaata ctctccaata ggtgaattaa atgaattttc gcaagaatat gctgaaacat
     6001 tagctgaagt actaggatat ggagtgttga taacagactt agattctata atagctgtat
     6061 ctaagcttcc taaaaaagat tacaaagaaa aaagtataag tgatgagtta gaagcaataa
     6121 taagtgaaag aacagtaaaa tattcaaaag ataaaaacat tttaccatta ttcaaggaag
     6181 accaaaaaga atatactagt caagttatca tgcctataat tagtgcatct ggtgactgta
     6241 taggttcaat tgtagttgtt tcaaaggata aagatacaat tgcagaaagt gaagaaaaat
     6301 ctttaaaaat tgctgctaac tttttaggaa aacaagttca ataattttat cataaagacc
     6361 ttttacatga aggtcttttg aagtttttgt gtaaaatatt gaatatagca ttaaaaatat
     6421 ggtatattaa agtttgttat ataaacagta tggaggttat gttatgaaca ataacaaaaa
     6481 taaagactcc tttctaaaag gagcccttat acttgggcta gcaggtgtaa tcgtaaaaat
     6541 aatgggtgga ttttttagga ttccccttgg aaacatgatt ggttcagaag gaatgggata
     6601 ctatcaggct gcttatcctg tgtatactct atttttaaca ttagctactg ctggatttcc
     6661 aacagctgtg gcaaaattgg tttcggaaaa agttgcaatt ggaaatttta aaggtgcaaa
     6721 taagatattt aaggtatcac atacagtttt attcattacc ggtataatat cattttgtat
     6781 attatttttt ggagccgact acatagtaac aaacgtaatg aaaaatccag gtgcgttata
     6841 ctctatgaaa gccattgctc cagcattact atttgttcct gcaatgtctg catatagagg
     6901 atattttcaa gggagacaag atatgactaa gattgctgtg tctcaagttg cagagcaatt
     6961 ttttagagtt gtattaggtc ttactttggc ctacttgctt atgaagagtc taggtcagac
     7021 ttttggtgca gcaggtgcta tatcaggggc tacaataggt tcaatagcat ctatgcttta
     7081 tttggtgttt gcttatatgc tagggagaaa agagcgtaga gcagagatag atgcaagcca
     7141 aagatttaaa gatgaaagag tgtcttatat ttttaaaaag cttttaactg ttgccattcc
     7201 tataacaata ggagcatcag taatgccact tgtaaatatg atagataatg ttattgtaat
     7261 aagaagactt atggaagcag gatttacata caaagtggca aattcaatgt ttggtcaatt
     7321 aacaggtatg gcaatggcca caataaattt accagcagtc ataacaactg caatgagtat
     7381 gagcttagtt ccagcaattt ctaaagcata tgcattagga aataagtcaa aagctagaaa
     7441 agatactaag agtgcagtta aggtaacttt acttatagta ttgccttgtg cgtttggtat
     7501 ggcttcttta gcaataccaa taatgggtct tttatttcca catgaaccat cttctgtagg
     7561 aactattttg tttactctta ctccatgtgt attattttta ggattaatac agacattgac
     7621 tggtataatt cagggaatgg gaaaaccaat agtacctgta attgctttat gtgttggtat
     7681 gctatgtaaa attgtaataa gttatacttt aacaggtata ccagatatta atgtactggg
     7741 ttcagcattt ggtacagtta cagcatactt tgtggcagcc atcattaact tactatatgt
     7801 taaaaaacac atgaatgtaa acttttctaa aaaagaattt ataataaagc catttataac
     7861 agttatgaca atgtttataa tggttaagtt gagttatggt gctttagttg gattcttagg
     7921 aaacagcata tctacaataa ttgcaatatg tgttggtgga attgtatatg tagtagtaat
     7981 acttggaata ggtggaatta aaaaagaaga gattctaact atgccaaaag gtgataaatt
     8041 atataaattg ttaaaaaaag ttaaacttat aagataatat aggaatatat atgcaaataa
     8101 aggaaatact atacaaatag taaatctcct tatttgtata ataatataaa aataaagtga
     8161 aatttgctaa taggagtgat gttatgggta agataagtat tgtaggtctt ggaccaggag
     8221 actattctct tataagccaa ggtgtgttag aatctttaat aaatagccct aaagtttttt
     8281 ttagaacaga gaaacatcca ataattgata aacttaaaga aactatacaa tacacttctt
     8341 tagactactt ttatgaaaaa gaggaaaatt ttgataatgt ttatttgaaa atatctgaat
     8401 ttatcataga agaatcagca aagggtgacc tagtgtatgc tgttcctggt catccaagag
     8461 ttgcagagaa gactgtaagt ataatagaga gattagccaa tgaaaagcac atagcagtag
     8521 aaatattgcc ttctatgagc tttgtagacg ctatgtttaa ctatttggct atagacccat
     8581 ctgagggatt taaactggta gatgcttttg aaatagaaaa ttcatatata gacttggata
     8641 caagcatgat aataactcag gtatatgata aatttatagc atctaatgta aagttaaaac
     8701 ttatggaaca ttatgactat aatcaagaag tatgtatagt aaatggagca ggtgtcaaag
     8761 atttagaaag taaaaagttt ataaagcttt gtgaattaga cagaaatgaa aacttttttg
     8821 actatttaac tagcttatat atacccaaaa gttctaaaaa gatgtataat acagtacatg
     8881 acttagaaat aatagttaat acacttagaa gtccatctgg atgtgaatgg gataaaaaac
     8941 aaacacatca atcattaaaa aattctgtta ttgaggaagc atatgaactt tgtaatgcaa
     9001 ttgataataa tgatatagat gaaatggtag aagagttagg tgatgttctt ttacaggtta
     9061 tattccattg tcagatagga aatgaggagg gctactttga tttaaaagaa gtagtaaatg
     9121 gcatatgtaa aaaattaatt catagacatc ctcatatatt tagtggtgca aaattagata
     9181 tgtctaagtt tgagaaaact tgggaagaat taaagaaaga agagaaaggc gaaagtacta
     9241 taacagatgg ccttagaagg ataccaaaac atttaccagc tcttatgaag gctagtaaag
     9301 ttcaacataa agcagcactt gtcggctttg attgggacaa tgtagaagat gtatttaaaa
     9361 aaattgaaga agaatatcag gaattacttg acgaacacag ggtcgggaat ataaaataca
     9421 taaaggaaga gctaggcgat ttattatttt cgatagtaaa tctagctaga tttttagaca
     9481 tagactcagg agaagccttg aactgtacta cagaaaaatt cataaataga tttgacttta
     9541 tagaaaaaag tgcaatgaac ttgaataaaa aattagaaga catgacactt gaagagatgg
     9601 atgagttttg gaatcaggct aaaaaacaat aaaattgtgg aaggaaattt gtcaaaaatc
     9661 tagaactttt atcacaaaga tagaagtcta tattataaac atttttagga ggtaaatatc
     9721 gtgaataaag ctgaattagt atcaaagatg gcagaaaaaa gtggattaac aaagaaggaa
     9781 gcagaagctg cgttaaacgc atttatgagt tctgttcaag atgcactagt aaataatgaa
     9841 aaagttcaat tagttggatt tggaacattt gagacaagag aaagagctgc tagacaagga
     9901 agaaatccaa gagatccaga gcaagttata gatataccag cttctaaagc accagttttc
     9961 aaagctggaa aaggattaaa ggatataata aatggataat tagaaatctt gtgaaaaagt
    10021 gtggataaat ttgccacact ttttctatag aaccaaggaa ggtgataatt atgagattag
    10081 ataaattttt aaaagtatct aggattataa aaagaagaac tgttgctaaa gatgcatgtg
    10141 ataaagggat tgtcactata aatggaaaag ttgctaaatc ttcttcagag gtgaatattg
    10201 gagatacatt agaaatacaa tttggagaga agaagatgaa gtttaagata aatgaagtaa
    10261 aagagcatgt attaaaaaat gatgcaaaag aaatgtacga aattttagaa taaaattaat
    10321 agtcctctag tatattaata gtagtattat tgaaatattt actgggggga tattatggaa
    10381 cagaacataa ctttgaaaga tagatcaaag ctggttattt cgggagtcga gcacatatac
    10441 tcttttaatg acaaaagagt agagctcaaa acttctgtag gcgagatggt aattgaaggc
    10501 gaaaacctag atatgagcaa gctaagtata gacgaaaata taattagcat agatggaact
    10561 ataaattcta tggtatatgc aaagcctaaa aagccacaag agagtttctt gaaaaaggta
    10621 tttagataga tgataccttt tactcaagat gtaagcattt tttatgctac tatttatggt
    10681 ggaatactca taggggtgtt gtttgatttt tatagagggc taagagggaa ttttaaattc
    10741 atcaattatt ttgctataat ctttgatgta ttgttttggt ttctagctac ggttataata
    10801 ttcgtaacaa taaaccttac agaatttttt gatttaagat attatcattt tgtggctttg
    10861 tttattggtt ttattttata ctataacacc ataagtaaaa ttgttttatc aattatcaat
    10921 aaaataattc gttttgtgag aaattctttt aaaaaagtta cacactacat agtcagtttt
    10981 ttaaataatt tgtattacgt tataatctat tcactacacc tactatttga tattatattt
    11041 tatataccaa acatatttat agcaacaaga aaaagtataa aaagaaaatc aaataaaaaa
    11101 ttaaagaaca agaaaaaatc aaaacctaag aagaaaaaaa ataaaactaa aaaaagggtg
    11161 taggaaaaat gaacttaaga aaaaaatttt caggacagat tattgttatc agtttatttt
    11221 taggaatatc aatttttagt atgatgacag gatttgtatt tgagtataca aaagctaagg
    11281 aatataaaaa agaaatagca agtctaaata aacaattaaa aaagactgaa atacaaataa
    11341 acagcttaaa gaaagatgaa aaatcatatg aaggagactt agaggatata gcaagaaaaa
    11401 gattgaatat ggtaaaacca aatgaaacag tttatgtaga cataaatcgt tgattaaaac
    11461 ttgctaataa gtttacagat gtattgattt taaaatttaa gatttaataa aaaagtcatt
    11521 cagttaagga tggctttttg tttttattga ggagggaatt tcataatgaa agtaggagct
    11581 atagatattg ggacaaactc aatgagatta ttgatagcag attatagaga tgggaatttt
    11641 ataaacagaa aaaaatttgt taatacaact agaattggtt atggtgtgga tagtaaagga
    11701 tatataaaag aagatgctat caaaagaaat ataaaagcat taaaagaatt ttctgataag
    11761 tgtaatgatg aaaagtgtga aaaaatttat tgtatgggga catctgctct tagagacagt
    11821 aaaaatggag attactttgt taaattagct aaagaagaaa ctggaataga tgttgatata
    11881 gttacgggaa tagaagaatc aaatttgggc tttaagggtg ttttagaagg aatacaaggt
    11941 gatgatacta ttattgttat agatattgga ggaggttcaa cagaatttat tgtaggtgat
    12001 tcagaaggaa ttatatttaa taaaagtgag aatgtaggtg ctcttaggat gacagagagg
    12061 tttttgaagc acgatattat tgaagatgaa gagtttgaag ctatgagttc atttatatat
    12121 ggtgagattg ataaaacgtt aaatcatata aaagacagag gattaaaaac tcttataggt
    12181 ataggaggta cgattacatc attatcagca atgaatcaag aattagagat ttactctatg
    12241 gaaaaaatac ataatagtaa aatacacaaa aaagaaatag aagaaattct acaaaaatta
    12301 ataaagatga cattaagtga taaaaagaac ttaaaagggc tacaagaaaa acgtgcggat
    12361 attataactg ctggtgtaaa aatattgaat ataataatgg aaaaattaga aatagaagaa
    12421 ataaccgtaa gtgaatacga taatttggaa ggcttaatat gtcaaaagtc aaaaaaaatg
    12481 tattaatatt ttttgatttt taaaaccgat tatgacaaaa aaaattactc ctctttgtta
    12541 caattcttaa tataaacaaa agaggaggtt tttttatgca aagaagtgta gcagttgtga
    12601 agaataagac tgtaaaacca aatgtcataa aattaataag cggatatatt gattttacta
    12661 cggcggcatt cgctgtgatg ggatttttac ttagtaggtc tattatagta gactctgtag
    12721 ctcctttagg aattgccttt tttatatgtg catcaaagat tgataagtac aagatacctg
    12781 tattcatatc aacattatta ggaatattgt tatcatctaa tgattttgga aatgtagcaa
    12841 aatatgctgt gtgccttaca atatttatgg ctatgaataa aaaattaaaa ttatcagatt
    12901 caattgctaa ggttgcattt ataggtgcaa taatactact tccaatctcc atgggacaag
    12961 ctttcttttc aaataggtat gtatatggtc ttcttatagc aggagtagaa gcaataataa
    13021 tgtttatatc tatatatgtg ttttcttatg ggataggttt aatggccaat gtcaacaata
    13081 ggatgtctgt acggacagaa gaagccatat caataagttt actgctaacc tttagcataa
    13141 tgggaatagg agatatatct ctgtttggaa tatcaataag gaccatttta tcgaccgtat
    13201 tgatattgct gacctcaata gtaggtggag ctaccatggg agcatctagt ggtgtaatta
    13261 ttggtgtagc atccatttta aataatatga catcagctgt ttatatgggg atatattctt
    13321 tttcaggatt aataagtgga gcatttaata aaataaataa atatttctgt atactaggat
    13381 atattctaag ttggacaata atatacctat atacttctgg tattacttct aatatgatgc
    13441 aattaagaga tatactatta ggttgtttga tagtactagt attgccagag cgattattta
    13501 ataagattga aaaattaata aaaagtaatg tggcttcaaa tgaaatcgta tacgattata
    13561 ttatgagaag caaaaaccta actaatagca gattgaacag tatttataaa acttatgatg
    13621 accttgcaga tacatttgat aagataagag aaaaagacaa agtgctagac caaagagaca
    13681 tagctaatgt aatagatatg attcataatg atgaatgtaa aagttgtagt atgagaagaa
    13741 tgtgctggga gtcaagattt aatcatacat acactatgat atataacatt cttgaaaaaa
    13801 ttgaggagaa aggggagtta agtctaaacg atatacctaa aaattttaga aaggaatgta
    13861 tgaaaccaga gtcaatagtg aaaatatcaa atcactatta caaaatgttt gtattagatt
    13921 atgattggag tgtgaagttt tcagaaagta gaaagttaat agcaaatcag ataagaagta
    13981 tttcaaaatc cattaaatcc ttatctcaag atttagaagg cgacataatg ctagatatag
    14041 aaaaggaaaa aaatatatat gaacagttag aaagatatga tataacagtt gataaagtaa
    14101 gttacttaac aaaaagtaac agtgagtttg aaatatcaat agaaaagaaa acttgccatg
    14161 atggatgtat gtgtgaagat aaaattgtaa atataatttc agacctagta ggggaaaata
    14221 tgtctgttag aaaaatagga tgtcactgtt taggaggtaa atgcaaggca acattcgtta
    14281 aatctcaaaa atataaggct gttacagaag tttcagcaat gtcaagggat gggcatatcc
    14341 tttgtggaga caattacaca tacatggaga taaatgatgg taaatacatg atggctataa
    14401 gtgatggtat gggaaagggt aaaaaagcat atgaagaatc ttctgccact attgatatac
    14461 tggaaaaaat gatagatgca aaaattaaag atgagatagt cattgatact ataaacaata
    14521 tgttgttgtt aaaatcttca gaagagatgt tttcaactct ggatttggga attttagatt
    14581 taaaacgagg ttgtttagaa actataaaga tgggagcgtg ttctacttat ataaaaagag
    14641 aggatggaga agttgactta atatcatcat catcattacc tgtgggtata ttatcagatg
    14701 taaaaattga tagaaaaaat gtaaaagtta aggaaggaga ctatgttata atggtttctg
    14761 atggaattgt agatgcagga agaaataaca atttaggaga caattggtta atatacttct
    14821 taaagaatat agagactaca aatccaaagg aaatttcaaa tttaatattg gatagagcat
    14881 tagagttaca agcgttacaa atcgaagatg acatgactgt tttagttacg aaaatatgta
    14941 caaaataagg tttacaagat tatgtgtata agatgtggat aacttgtgta taatatgtta
    15001 atagactttt gaaaaaagta tagttattaa caaaaataac atataaatca acaaaatttg
    15061 ttgataagta tggcagaaat gttaataaaa tgtaaaaaaa gaaaaaacaa aagctattat
    15121 caaaagagat tcatgttgaa ataactaaat ttctaagtaa agaaaacctg tgaatatgtt
    15181 gattattatg tggataacta ttaattcgat atttatttat atagtatgaa taattgaata
    15241 atatatctaa aaaaagccat cctattatta agtaaactta ttgaaaggat ggctttttat
    15301 gaatagaaaa ttgtgcaaag caataatacc tttatttatt tctatttttt gcatgttaat
    15361 aataagtata aaagctgacc aaggatatca aaagaataat gacagagcaa ttactgtcat
    15421 gaataataat gatactgaag atgatgctga aaaaaaacaa gaaatagact atgaaaaaac
    15481 atgttggaat ttgcaatctt tgtttaaaag tgatgaccaa tggaaaaaag aacttaagag
    15541 ttttgataaa gatactaaag agttgaaaaa ttacataggt aaagtaacaa aatcgccaac
    15601 acatttgtct tttgccttaa acattaaaga aaaacttgat ataagactaa ataaactttc
    15661 tgcctatgta aaattaaaac aagatactaa taaaaattcg tataagtact tagatatgaa
    15721 tgatagtatg agtaaatcct atgggaatta tatggggatt tgttcagatt tagagttaga
    15781 aatactaaaa ttatcaaaca aagattacaa gaaaattatt tcaaataaaa atataaatag
    15841 gaaatatggt gcatatttaa gagatataag aaggaataaa gtacattatt tggatgataa
    15901 atcagaagat attctaagca aagtatcttc aataagtagt cttccaagcc aagtgtatga
    15961 attatttaga aatatggata gaaaaactaa tttgacacca gctcaatatg cgagtgaatt
    16021 agagtcatca gatagagaaa atagaaaaaa agcttatcaa gatgaactta taacttacaa
    16081 tgataatata aatacaattg caggtttaat tactggtcaa gttaaaaaga atatttttta
    16141 ttctcaggaa agaggttatg agtcttcttt agaaatgtac ctggaatctg ataatgtaga
    16201 tgaaaaagta tacaataatt tgataaggac tgtaaatagt aatatggaca gtctacacaa
    16261 atatatagat ttaagaaaaa aagtattaaa attagataag gttcattcat atgatatgtt
    16321 tgtgcctatg gttaaggctg ttgataataa tattaattat gaaaaggcac aaagtatagt
    16381 atattcagcg ttgtcacctt taggaaaaga atacaatgat gtagtatata aagctttcaa
    16441 tgaaaaatgg atagatgttt attcacatga aaataaagta agtggtggat actgcttatc
    16501 tgtttatgaa aatcatccat atatactttt aaattataat aattcattag gttcagtttc
    16561 gactttatca catgagcttg gacatgctgt atatgaatac ttaagttcta agaatcagaa
    16621 ttattttaat tcaaatccgt ctatatttac tcacgaagtt gcatctacaa cgaatgaagc
    16681 attactttac gagatgctta taaaagaagc taaaggcaat gatgaaaaag cttattatat
    16741 aacacagtat ttagacttga taaaagatac tctttataca caaactatgt atgctgaatt
    16801 tgaaaaaact atacacgagt tagtagaaaa aggaaaaagt gtaaacgcat tagtattaaa
    16861 tgatgtttgg ggacaacttc ttaaaaaata ttatggacaa agctatgaat tagaccaatt
    16921 atctaaggtc ggatgggcta gaatcccaca tttttataat agtttctatg tctataaata
    16981 tgctacaggt tgtagtgcag gagtaagttt tgcccaagac atcctaaaga atggttcaga
    17041 taactatatg agctttctta aaaagggaag ttcagattat ccaataaact tgttaaaatc
    17101 aagtggcata aatttaacta atacaaaacc catagaaaat acaatcaaaa aatttgatag
    17161 tttagtagca gaacttgaaa agctattata aacattattg taatccacat ggtaaatata
    17221 tcgaattaat gataaaatat actaaaagtt taaagtccta tcaagatagg actttttatt
    17281 tattaattag tgggagatga ttctatgcaa gttaaagtta aaaaggctgt aataccagca
    17341 gcaggtttag gaactagatt tttgccagct acaaaggcac aaccaaaaga gatgttacca
    17401 atagttgata agccaactct acaatacata atagaagaag ctgtggcctc aggaatagag
    17461 gaaatactaa taataacagg tagaaataaa aaatctattg aagaccactt tgataaatct
    17521 gtagaattgg aacttgattt agagaaaaaa ggtaagaaag aattgttgga ggtagtacaa
    17581 aacatatcta acatgattaa catacattat ataagacaaa aagagccaaa agggttggga
    17641 gatgcaattt attgtgcgag gcattttata ggtgatgagc cgtttgcagt aatgttaggg
    17701 gatgatattg tagataatga tgtaccttgt ctaaaacaac taacagatgc atatgaggaa
    17761 tatagaacta caatattggg tgttcaaaag gtaaatcaag aagatactaa taaatatggg
    17821 ataatagaag caaaaaatat agaaggtaga gtttacaagg taaaagacat ggtagaaaag
    17881 ccagagtcag gaaaagctcc ttcaaatatt gctattttag gtagatatat aataactcct
    17941 gaaatctttg atattcttaa ggatttacca ccaggtaaag gtggagaggt acaacttaca
    18001 gatgctttaa aaatattatc taagaaagaa gctatgtatg catacaattt tgaaggcaag
    18061 agatatgatg ttggggacaa gctaggattt ttagaagcta ctgttgactt tgctctaaag
    18121 aaagaagacc ttaaggaaga ttttataaaa tatttgaaac atgtatgtag tgaatttgat
    18181 aaaaataaca atgtgttata caataataca gatgaaaaaa tagatttaat tgaagtcaat
    18241 gaagctagag aacttgaaat tcaaaaataa ttaatgaaag gtgatgtatg gtggcaaaat
    18301 caagtcagaa aagaattaat agaataatag ctgttgggat tgtgggaatc ctcgtaataa
    18361 cgagtataac agctgttttg agtatgattg caactatgta ataagataaa tgacagcagt
    18421 tgatatacat aaatataaaa accatatata acttgatgta gtaaaaaact tacaatattt
    18481 tagttatata tggtttttag aatttataaa tgtaaaaaaa aatttttata atagttgcat
    18541 attttaaagg aaaatattat tataagatat aaatatagtg ataaatttat aaaaacgtgc
    18601 taagattgga cttgaaatac agtaatatag taggtaataa tgttttaaat tttatttatt
    18661 gaccataata tataaataat gatattatta gacttaactg tttaaataaa acttttcttt
    18721 ttcatgcctt caatttccaa aataagtatg tatataaatg aatatataga tgaggaggat
    18781 ttttttgatt ttagatgata aagtattaga aggattaggt agtaagacct tagttgagct
    18841 acgagagata gctaaagaat taaaaataaa atcaattact acatataaga aaaatgagtt
    18901 aattgagatt ataaattcca aaagtaaaaa tgaggaaaaa gtaaacgaag agactaaaga
    18961 aaaaaatgaa gatattaaag aagacataaa gaatagaaaa gaaaatatag agcgtgaaag
    19021 tatagaaaaa gaagttgaat ataagagccc taccaaatca tacaacaaga aagaaataga
    19081 tacccaaaat atttcccaaa accaagaaca tagaaatcaa agaaatgact ataataagac
    19141 aacgagtgta aatgatagca ataagtcatc aaatatatct aatagaatgg tcagaaataa
    19201 taataaaaac tattatatgc ctaaacaagt tgatgaatct aagatagtag atgaatttaa
    19261 cacatcaaaa gaggatgaag tagtaggtgt acttgaaatt ctgccagatg gatttggttt
    19321 tttaagaggt tcaaattatt tgtctactga aggagatgtt tacgtttcac catctcaaat
    19381 aagaagattt aatatgaaaa caggagacaa gattaaggga ataactagac atccaaaaag
    19441 tggagagaaa tttagagcac ttttatatgt tcaaaaaata aatgatgaaa accctgacac
    19501 agctatacaa agaaatgcgt ttgagacatt aacaccaata tttccagaag aacgacttac
    19561 actggaaact aatagaaatg aaatagctac aagaattata gatttaattt cacctatagg
    19621 aaaaggacaa agaggattga tagtagcacc tccaaaagct gggaaaacag ttcttttaaa
    19681 aagcgttgca aatagcatag ctaaaaatca tccaaatgtt gaacttatag tactccttat
    19741 agatgaaaga ccagaggaag ttactgatat gaaagaatct attgaaggag acgttatata
    19801 ttccactttt gaccaagtat caagtcatca tgtcaaggtt gcagagatgg tgttaaatag
    19861 agctcaaaga ttagttgaac atggtaaaga tgtagtaatt ttactagata gtataactag
    19921 attggcaagg gcatataacc ttacaatatc tcctacaggt agaactttat ctggaggtat
    19981 tgaccctggt gcacttcatg gacctaaaaa gttctttggt gcagcaagga atattagaca
    20041 gggtggttct ttgacaatat taggaacagc attggttgaa actggttcta ggatggatga
    20101 tgtaatattt gaggaattta aaggtacagg aaatatggag ctacatttag atagaaaatt
    20161 agcagaaaaa agaatattcc ctgcaataga tatttacaag tctggtacaa gaagagatga
    20221 tttattgctt gatgatgaag aaaagactgc tctttggaga ttaagaagag aaatgagtaa
    20281 taattcagta atggaaatta cagataaagt cattgaactt ataaagagaa ctaaggacaa
    20341 taaagagttt gtaaaatcaa taaagaattt gtaaaatcaa taatacaaat gcaaatatgg
    20401 attatataaa ctaatagaaa aatcacttga atggcataat aagttgtgct ataataatgt
    20461 agttgataat gtaaaaagaa agttaatata attaattttt ttaaataaag agaagaggtg
    20521 actataatgc aaaaggaaat acaaccaaaa tacaatccag ttgaagtgcg ttgtgcttgt
    20581 gggaacacat ttgttgctgg ttcaactaag gacgaaataa aagttgaaat atgttcagag
    20641 tgccatccat tctatacagg aaaacaaaag aacatagaaa aaggtggaag aatcgacaaa
    20701 ttcaagaaaa gattcaaaat ggactaattt ttataaaagt ggagttggag gaagccaact
    20761 cctattttat tacaaaaaaa cattcatatt ctactttaaa ttgtcttttt gtattataat
    20821 aataaatatg taaaattgag tattctaaat acaataaaat gaggtgagtg gatggcaaaa
    20881 caagctgtag gtgggcaagc tgttattgaa ggtgtaatga tgcagtcaaa agacaaaaga
    20941 gcggtagctg ttagaaaaag tgacggagaa atagcactaa aagaagatag aataaaaagt
    21001 tgggtaagag ataagaatat agataagata ccttttgtta gaggttcttt tgttatgata
    21061 gatacaatga ttcaaggcat aaaaagctta aacttttctt cagagttttt tatggaagaa
    21121 gctgaagagg ataaatttga tttatttata aagaaaattt ttaaagataa ggcaaatgat
    21181 ataattataa tattttcatt ggtcatagca atgttacttt ctgctggatt attcattttt
    21241 atacctacat tggtaggagg agctttttct aaagtaatgc ctaatgattt tatgcttaat
    21301 ttaatagaag gaatcataag gatagctata ttatttgcat atatagtatt gatttcacga
    21361 agtaaggata tagaaagagt ctttcaatat catggagcag aacataaatc tatatattgc
    21421 tatgaaaatg atttagaact tacagtagag aatgctagaa aatttaaaag attacatcct
    21481 agatgtggta caaatttcct atttatagtt atggctgtat caataatatt gtttgcattt
    21541 tttggttggc caaatccaat tcttagaata tttatgagaa ttatatgtgt gcctatagta
    21601 gcaggtttat cttatgaggt aattagagtt cttgggaaat atgataatgg atttactaaa
    21661 attattgcat atccaggaat gatgttgcaa tattttacaa caaaagagcc tgatgatgag
    21721 caattagagg ttgctttgga agcattgaaa gcagtagtag attagagaaa aggatatttg
    21781 ttatgacgat aaaagatata attattaaat attcagataa actaaaagat ataagtgata
    21841 ctccaagatt agatacagag ttattgttac aaaaaacatt gggtgttgat agattatata
    21901 ttcacttaaa tctgaataaa gagctaactg aagaacaaaa aacaaaattt atgggttttg
    21961 cagaagaaag attaaatgga agaccaatag catatatagt tgaaaatagg gagtttatgg
    22021 gattagattt ttttgttaag gaaggcgtat tgattccaag accagataca gaaactttag
    22081 tagaagaaat aattgaaata tgtagagaaa aaaaagatgt aagtatatta gatataggaa
    22141 ctggttctgg agctattaca ataagtttag caaagtacat tgaaaattct aaaatcatgt
    22201 cttttgatat atcagagaca gcattagaaa tagctaaaaa aaatgctatt ataaatgaag
    22261 ttggtgaaaa aataaagtat attaattctg atttatttac agcaataagt gatagtaaca
    22321 taaaattcga tatcatagtt tcaaatccac catatattaa aaaacaagat atagaaactc
    22381 tgcacaaaca agttaaagat tatgaaccat ataatgctct tgaaggtgga gaagatggat
    22441 tagactttta tagaagaata acagaacaag gtaagaaata tttaaataag tgtggaatat
    22501 tagcatatga agttggtcat aatcaagctg aagatgtaat aaatattatg aaaagtaatg
    22561 gatataaaaa aatatacaca aagaaggata ttcaaggcat tgatagagtt gttataggtt
    22621 acaatatatg atataatcaa caattgatat gttacattta atcgaggtga aagtatgtta
    22681 aaaaaattag aggtattgga agatacatat aaagacttaa gtgaaaaaat aggtgatccg
    22741 gatgtaataa atgatcaaaa agtttggcag aaatatataa aagaacatgc tgacttggaa
    22801 cctattgtta tgaaatacag agagtataaa agcgttttag atagtataaa agaatcaaaa
    22861 gaaattttac aagaagagtc agatgaagaa ttaagagaat tagctaaaat ggagttagct
    22921 gagatggaag aaaaagtagc acctctggag gaagaaataa aaatactatt attgcctaaa
    22981 gaccctaatg atgataagaa cgttatagtt gaaattagag gtggagctgg aggagatgaa
    23041 gcagctctat ttgctggtga tttatttaga atgtactcaa ggtatgctga aagaagaaga
    23101 tggaaaatag aattattgag tgcaagtgat actggagttg gtggatataa agaagtatct
    23161 tttatgataa aaggtaaagg tgcttattca agattaaagt atgaatctgg agttcataga
    23221 gttcaaagaa taccatcaac tgaatctggt ggaagaatac acacatcaac ttctactgtt
    23281 gcagtattac cagaagttga agatgtagag gttgaaataa atccaaatga tttaagaata
    23341 gatgttttcc gttcttcggg taatggagga caaagtgtca atactactga ctctgctgtt
    23401 agggtaacac atatacctac aggtgaagta gtatcttgtc aagatggaaa atcacaatta
    23461 aaaaataaag agcaagcttt aaaaatatta aaagctagac tttacgataa agcattggca
    23521 gaacaacaca aagatatagc agcagaaaga aaaagccaag tcggaacagg agacaggtca
    23581 gaaagaataa gaacatataa tttccctcaa ggtagaataa gtgaccatag aataaattta
    23641 actttatata agttagatgc attcttagat ggagatatag atgaaatgat agatgcttta
    23701 attactgttg accaaacaga aaaaatgact gcaatataag taatgtttta aagaagctgt
    23761 ttcttcctag aaattatttt ctattttgaa acagctattt tgcattttag gtcatgttat
    23821 catattctat tgtatataat tgttatatac aagtatagga gggttatatg atactaaaag
    23881 taacaataat tgggcttttg gcaggtgtta tagggacagg attaggagga gtaatatcag
    23941 ccatttttaa aagagaggtc gataaatatc taagtttttt tatgggatta tctggtggaa
    24001 taatgttggc tgtagtggtg tttgatttaa tgaaagaatc catggacaaa atggggataa
    24061 tcaatacagt tatatttacg tttgttggag ctctcataac tatgtacata aaaacaaaat
    24121 tagatgtgtc tggaaatatg gcatctggat atcttatatt cataagtata ttactacata
    24181 atttaccaga aggactagcc attggttctt cttttatgtc aactgaaagt ttaggtatta
    24241 cacttgctat agttataggt ctacataata taccagaagg gttagctatg gcattaggct
    24301 tagtttgcaa taaaatgaaa ctaagtaaag ttattttgtt tactgtaata gctggattac
    24361 ctatgggtct aggaagtttt ttaggtgttt attttggagg agtatttact tctcttatag
    24421 gagtatttct tgctacagca ggaggtacta tgatgtatgt tgtattggaa gaaatttttc
    24481 cacattcaaa aagtgtgtat tgtataattg gtttcttgtt aggtacaatg attgtaaatt
    24541 atatataaaa ttaaaatgaa agcaggcata caaaagatga aaactattat aagtaatatt
    24601 gatataaata atattgatta tgaggaaata aaaatacaag caaaacttct tagagaaggt
    24661 aaaacagtta tttttccaac tgagacagta tatgggttgg gtgcaaatgc attagatgaa
    24721 aatgcagtaa agaagattta tgaagctaaa ggaagaccta gtgacaatcc attgatagtc
    24781 catatatatg aaaaagaaga agtatatgac ttagccaaag atataagtga taaagctaaa
    24841 cttgttatag aaaagctttg gccaggacct ataacaatta tattaaataa aaaagatata
    24901 attccataca aaactagtgg agggcttgat acagttgcaa taaggatgcc ttctaatgtt
    24961 attgccaggg caataataaa agaagctggt atacctattg cagctccttc tgcaaatatc
    25021 tcaggtcgcc catctccaac aaaagcaaaa catgtatatg aagagatgaa tggaagagtg
    25081 gatggaatag ttttaggagg agacagtaat tttggattgg agtcaactgt attggattta
    25141 acagaagaga ctcctatgat actgagacca ggaagtataa ctaaagaagt tttagaggaa
    25201 cttcttggag aagtaaagct tgacccatca ttaagtaaaa aagaagataa tcaaaaagct
    25261 aaagctcctg gaatgaaata taaacattat tctccaaatg cagatgtata tataatatct
    25321 ggaaaaagag aaaatgtagc atcaaagatt aatgatatga taaaatctaa tagagacaaa
    25381 ggtctaaaga caggtgtaat gtgtattagt agaaaccgag agttttatga tggagaagta
    25441 atcgatttag gaaactctct tgaagaagtt gcaagtaatc tgtttgatgc tcttatagaa
    25501 atggacaaaa agggtgttga tgtcatatat tcagaagagt tccccaagac tggagttgga
    25561 caagcaatta tgaatagact actgaagtca gcaggataca aagtgataaa ggcttaagag
    25621 ggagttgtaa aatatgaata tacttattgt gtgtacaggt aatacttgta gaagtcctat
    25681 ggctgaggct atactaagga aagcaattaa agaatctgga agaagtattg aagaatattc
    25741 tatatcatca gcaggtatat ctacagcaaa tggaatgggt gcttctgaaa attctataga
    25801 agtactaaaa gaaatcggta tagacttatc aaatcataga agtaaagtaa ttactaaaaa
    25861 attaattgat gaatcagata taatactaac aatgactaaa tctcataaag aaattttagt
    25921 acaagcagtt ccaaaatgca aagaaaaagt gtatactttt aaagggtttg caaataaaaa
    25981 tgaggaagat atatctgacc catttggtgg aaatttggat atttatagaa gcactatgag
    26041 agaaataatg tattctgtca acgaaatagt aaaaaaaata tgaaacggag gataaacaaa
    26101 tgaagatagg attaggctgt gaccatggag gatataattt aaaaaaagaa ataataagct
    26161 atttagaaag taaaggtata gagtgtgtag attatggaac taataatgct acagattctg
    26221 ttgattatcc tgtttatgga gaaatagttg caaattcagt tataaataaa gaagtagatt
    26281 atggaatatt atgttgtggt acagggatag gaatatcttt agctgctaat aaggttcctg
    26341 ggataagatg tgcagttgta tcagatgtat tttctgcaaa aatgtcaaaa gcacataatg
    26401 atgcaaatat gttatcatta ggcgaaagag ttttaggaaa aggtctagca cttgagatag
    26461 ttgaagcatg gattaatacg gattttgaag gtgatagaca tgctagaaga gtaaacatga
    26521 ttaaatcaat agaagaaaaa cataataaat aataaaaatt acagttttat caggaggaat
    26581 tatcaaaatg agcaaagtgg tagagacaaa ccatccatta atacaacata aattaacttt
    26641 aatgagagat aaaaatactg gttctaaaga ctttagagag cttttaactg aaattgcaat
    26701 gttaatgggg tatgaaataa caaaagatat accattaaaa gatgtagaaa tagaaactcc
    26761 tattcaaaaa acatcatcta aagtagttgc aggtaaaaaa ttagcaataa ttccaatttt
    26821 aagagcaggt cttggaatgg tagatggatt agtgagctta atgccagcag caaaagttgg
    26881 acatgtagga ctatatagag acccagaaac attaaaacca gttgaatatt attgcaagct
    26941 tcctcaagac ataggagaaa gagatataat agtagttgac cctatgcttg caacaggggg
    27001 ttctgctgta gcagctatag atttacttaa gtctaaagga gctaaaagta taaaattagc
    27061 taatttagta gctgcaccag aaggtatagc agaagtgcaa aaatatcatg atgatgttga
    27121 tatatatgtt gcatcagttg acgaaagatt aaatgaacat ggatatataa ttcctggtct
    27181 tggtgatgct ggagatagat tgtttggaac taaataaaaa taaataacta taaatattta
    27241 ttagggactt tcttatatag tgtatataat tttatgtaag aaggttcttt tttatatatt
    27301 ttttctcaaa ataggaatta ttgaattgat aaatttctcg aattgagaat aaaaagtttt
    27361 taccaattaa aaaagattgc ttattttaca agattttata gaatagtagt ctattttaat
    27421 gtggcatgtt agttgcttac gattaagatt ataggtatta agtgtattag ttaaattgag
    27481 aattgaatat taataatatt attgggagtg gtaatacatg aagaattgta tagtagtaag
    27541 aggggctgga gatttagcaa cagcagttat tcataaatta cacaacagtg gttttaaagt
    27601 agttgcgtta gaaatagaag agccattagc tataagaagg caaatttctt tttgtgaggc
    27661 tgtttatgaa aaaaaagtaa ctgttgaagg tgtagaatgt atattttgca atactgaaaa
    27721 agaaatagaa gaagttttat ctcaacataa agtagccttg attatagacc caaaaggaga
    27781 aaaattatct aaattaaatc caatcgtttt ggttgatgga atactcgcta agaaaaactt
    27841 aggtacaact aaagatatgg caccacttac aatagcttta ggtcctggat tttgtgcagg
    27901 agaagatgta gatgttgtta tagagacaat gagaggtcat aatctaggaa gaataataac
    27961 taatggtaga gcagaaaaaa atacaggagt accaggagca ataaaaggtt tttctaaaga
    28021 gagagttatg cattcacagt atgatggcat aatggaaaat gtatgtaaga taggtgatat
    28081 agttgaaaag ggagacctac tttgctatat taataattat gaaaaaaaac atgaagttag
    28141 agcaacaata tctggagtcc ttagaggttt attaaaggat ggagcaaagg taaacaaaaa
    28201 tttaaaaata ctagacatag accctagaaa agaagaagtt aagaatgctt ttacaatatc
    28261 agataaagct agatgtatag ctggaggggt tttagaagcc atactaaaca atgggattta
    28321 tccccaaaat taatttatat tagataaagc tattaatatt ttattaagat gatataataa
    28381 atttaataga ttaaagagag catatgacgg gtatttaaaa tttaaatggt aaaaatgctc
    28441 tttttttatt ggacattcaa ttaacatgtg tatataatat aaaaataagt atttatttag
    28501 gttgaaatct aataaatata taataccagt acataagcaa agaggagaaa agtaaattat
    28561 gagaccatca tgggatgaat acttcatgga aattgcagaa gtagtgaaaa agcgttcaac
    28621 ttgtattaga agacaagtag gagctgtaat tgtaagagac aaacaaatat taacaactgg
    28681 atataatggt tctccgagaa atcttgaaca ctgtgaaaat attggatgta agaggcagga
    28741 attaaatatt ccctcaggag aaaggcatga attatgtaga gcattacatg ctgaacaaaa
    28801 tgcgataata caagctgctc ataatggtat tagtgtagat ggggcaactt tgtatgttac
    28861 tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat gcaggtatat tgaaaattgt
    28921 ttatgaaggt gattatccag atgatatgtc tacagaatta ttaaaagaag caggaataga
    28981 actcataaaa ttttagtgga taaaatttat aagacaataa atgtcaaatt atgacactga
    29041 aatacaatgg tatcaaatga ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa
    29101 gatatatttt ataattaaat aaatttatga gatagattaa ttacatgtta tttattttat
    29161 aataatgagg gatactattt atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt
    29221 ttagtttaat aagtcaaata ggattgatga ttgtaatatc aatattagga tgtactttct
    29281 taggaaaatt tttagatagt aaattgaata ctactccagt tctgacaata atatttttat
    29341 tgttgggtgt tggaggtgct tttagtggtg tttataaaac tctaatagta tatacacgaa
    29401 ggaagtgata tattggatat aaaattactt aaccaggtaa ggtcagtaac aaaaggtata
    29461 gtaatatttg atgtagtttt aattgtgcta atgttgataa catcaacatt aagtaaacca
    29521 gtattattag gacttatatt tggctctata atagctatgt taaattttag attattggcg
    29581 atatcactgg aaaaatcagt gacatttcct gctgggaagg cacaagctta tgcatcagca
    29641 caatatgctg ttagattatt tataatggca gttgttctaa ttgcatcagc tacagtacca
    29701 catttaaata taatcggaac agcattggga ttattgagca caaagtttgt catattatca
    29761 aaaaacttta taaataaatt aaagaggaag gaggcataag tatgaatcaa gcgcattatt
    29821 atctcatagg aaactttaag attagtgaaa caatactagt aagttgggga ataatggcct
    29881 ttttagcgat agcatcttat tttttgacta gaaacttaaa aaaagttcca actagtaaag
    29941 ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa aatggttaca gatgttatgg
    30001 gtaaaaatac agtaaaaaga atgccatata tgataccata cataggaagc ttgtttttat
    30061 ttttcgcatg ttcaaattta agcccattac ttggattaag gtcaccaaca accgacttag
    30121 acacaactgc tgcatgggca atgattacat tttttatgat ttattttgca ggagttaaat
    30181 ttaaggggtt atcatacttt aaagaattgg ctcaaccact tgccataatg ttaccaatca
    30241 atattatagg tgagttagct agaccaatat cattaagttt ccgtccattt gggaatattt
    30301 taggtggaac aataataatg ggtctgtttt atcaactttt aggttttata tctagtttaa
    30361 taccaggatt atcaatacca attggagaaa tattaatacc agtcccatta cacttgtact
    30421 ttgacttatt tgcaggattg ttacaagcat atatatttat aatgttaaca atggtattta
    30481 tatcaagtgc aggagagtta gattaagaaa tcaacttata ttaagataaa aataaaataa
    30541 tttaaaaaat taaagtttta aggaggaaat aattatggaa acagcaatag tagcggcagc
    30601 atcagcaata ggagcaggga tagcagttgc aacaggtata ggtgcaggta taggtcaagg
    30661 tatagcagca gctaaagctg cagaagcagt aggaaaccag ccagaagcta aaggtgatat
    30721 aacatcaact ttattactag gagttgctat agcagagtct tcagcaatat atggtttggt
    30781 tatatcaata atacttctat tcgtaaatcc attctttaaa tacttaggaa tgtagattta
    30841 gtccttaaat attttgagat aggtttatag gtaaatcatc tcaaacttat aatcatgaga
    30901 tgaactttat catctcgtga ttataagtag ttatgattta tggaaaggag gataattatg
    30961 gaaaaagcac tagtaggtat aacgtgggaa tttgtgttcc aaattgttaa tacttttata
    31021 atatttttac ttctgagaaa gcttttattc aagccagtat taaatataat tgaatcaaga
    31081 gaaaatgata ttaaaagtga tttggcagaa ggcgaaaaag caaaaaatga aggtttagct
    31141 ctgaaaaaag aatatgagtc aaaaatcaat tttgccaaag atgaaggtca agaaatcata
    31201 aaacaagcaa ctataagagc agagcagaaa tcagatgata tagtaaatac tgcaaagaaa
    31261 gatgctcttg atataaaaga aaaagcaaac aaagatatag aacaagaaag acaaaaggtt
    31321 ataaatgaaa tcaaaaatga tatatctaac atagcactac ttgcagcatc taaagttatt
    31381 gaaaaagatt tagataaatc taaacatgaa gaattgatag aaaactttat aaaagaggta
    31441 ggggaagcta aatgataaat gtaatagcaa atagatatgc cgaagcccta tttcaattag
    31501 gagaagaaga aaattcaact gatgtcttat tcaaagaact agaaaaagta gtagatatga
    31561 tgactaaagt tagtaaagat ttctacaaag ttttaaaatc accattagtt tcaaaatcag
    31621 aaaagaaaaa cttggtagaa attattttta gtaaagaagt aagttctaac ataaaaaact
    31681 ttttaaaagt tttagtagat aaagatagaa tatcatattt agaagatatc gaattagctt
    31741 ataaagaatt attaaataag aaaaacaatg taattgatgg agtagcaata agtgctattc
    31801 caatgagtga aacagatatt aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa
    31861 atgttactat agaaaatgtt gttgacaaga ctatactagg gggagttcta gttagaattg
    31921 gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat
    31981 ctgaagtaat atcttaagaa tggcggtgaa cccatgaact taaaacctga agaaataagt
    32041 tctataatta aacagcaaat aaaaaattat gagaataaag ttgagttgac agatacaggt
    32101 agtgttttaa cagttgggga tggtatagcg agtgtatatg gattagaaaa agctatgtct
    32161 ggtgagttgc tagagttccc aggtgaaata tatggtatgg cacttaactt ggaagaagaa
    32221 gtagttggag ctgttatcct tggagatgac agtgaaataa aagaaggcga tatagttaag
    32281 agaactggaa gaatagttga agttccagtt ggagaagctt taataggtag agttgtaaac
    32341 tcactaggtc agccaataga tggaaaagga cctatagcgt acacaaaaac tagaccagtt
    32401 gaatctgagg cacctggtat aatagataga aggtctgttt atgaaccatt acaaacagga
    32461 atcaaatcaa tagattcaat gataccaatt ggtagaggtc aaagagagtt aataataggt
    32521 gacagacaaa ctggtaaaac atctatagtt attgacacta tactaaatca aaaaggaaaa
    32581 gatgttatct gtatatatgt agcaattgga caaaaacgtt ctactatagc tcaattagtt
    32641 agctctttag aaaaaggtgg agctttggat tatacaatag tagtttcagc aacagcatct
    32701 gaatcagcac cacttcaata tatagcacca tatgctggag cagctatggg tgaagaattt
    32761 atgtacaatg ggaaacatgt acttatagtg tatgatgatt taagtaaaca agctgttgca
    32821 tatagagaaa tgtcattgtt acttagaaga ccaccaggac gtgaggcata tcctggagat
    32881 gtattctatt tacattcaag attacttgaa agagcagcta agttatctga tgaattgggt
    32941 ggaggttcaa tgactgcctt gcctataata gaaactcaag ctggtgacgt ttctgcatat
    33001 ataccaacaa atgttatatc tataacagat ggtcaaatat acttacaacc agagttattc
    33061 tattcaggtg taagaccagc agttgaccct ggtatatcag tatcaagggt tggtggttct
    33121 gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa aacttgcata ttcacaatat
    33181 agagaacttg cagcattctc acaatttggt tctgacttag atgaagatac taaaaagaga
    33241 cttgctcaag gtgaaagaat cgttgagata ttaaaacaag gtgagcatca accaataaaa
    33301 gttgaaaatc aagttatgat aatatatgct gttataaata atcatttaga ggatattccg
    33361 atagataata tagctagatt tgaatcagaa ttgtatgcat ttgtagataa taattatccg
    33421 gaaatatcaa gaaaaatatt aggtggagaa gacttcactc atgatttaac agatgcaata
    33481 aatgaattta aagaaaagtt tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat
    33541 taattggcag gagctggaat caaagcaatc aaaacacgta tgggaactgt tgaaagtaca
    33601 aagcaaataa ctaaagcaat ggaattagtt gcatcttcta agcttagaaa agcaaaagaa
    33661 aaggctgaag attcaagaga atattttaat actttataca atacagtaaa agatattgcc
    33721 caaacaacac gtggaattag aaatgaattt ttaaaagaaa gagaagtaaa aaataaatgc
    33781 tatgtagtag tagctggaga cagaggactt gcaggtggat acaattctaa tgtattaaag
    33841 gaatctgtct ctcatatgaa aggtaaaaag gaaacagtga ttactgttgg aaagaaagca
    33901 catgaatttt tttctaaaag aaactacaat atagcaaaag atataaatag tgttgaagct
    33961 tgtggatatg aagatgcgac agaaatttca caaactatta ttgacctgta taaaaaaggt
    34021 gaagtagatg aagtttatat tgcttataca aaatttaaat ctgctttatc acaagaagtt
    34081 gaaattttga aattgcttcc attagtcttt gaaaaagata cagacgaaca aggaatggca
    34141 gtaaaagaag aaaaacaaaa tagatcaaaa gtacaatata taccatcagc agaagcagtt
    34201 ttaagttata ttgtacctca gtttatatca ggtacagtat atggtggaat tttggaatct
    34261 tttgcatcag agcaaggtgc tagaagaact gcaatggaat cagccacaga caatgcgaat
    34321 gaaatgctat ctaatttaga gttaagttat aacagagcaa gacagtcagc tgtaactcaa
    34381 gaaataacag agatagttgg tggagttgaa gctctaaaat agggaggtta ggaaatggca
    34441 aatgtaggta aagtagtaca gattgtagga gctgtactag atgttaagtt tgatagtgaa
    34501 caaagcctac ctaacttatt aaatgcgtta gtaataaagt taggagataa agaaatagtt
    34561 gcagaagttg ctcaacatat aggtgatgat actgtaagat gtatagctat gagtgcaaca
    34621 gacggacttg taagaggtat ggaagttgta gatacaggag ggccaataag tgttcctgtt
    34681 ggagatgaaa ctttaggtag aatattcaat gttcttggaa aaccagttga tgggaaacca
    34741 gctccaaaat ctgcacctaa attacctata catagaccag caccggcgta tgatgaacta
    34801 gaaactacag cagagatact agaaactggt attaaggtag ttgacttact agctccatat
    34861 ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag taggaaagac agttctaata
    34921 caagagctta taaacaatat tgctaagcaa catggtggta tttctgtatt ttcaggagta
    34981 ggagaaagaa caagagaagg taacgacctt tatggcgaaa tgagtgagtc tggagttata
    35041 aataaaacag ctctagtatt tggtcaaatg aatgaaccac ctggagcgag aatgagagtt
    35101 gctttaactg gacttacaat ggcagaacat tttagagatg agcaaggaca agacgtttta
    35161 cttttcgttg ataatatatt ccgtttcaca caagctggtt cagaagtttc agcacttcta
    35221 ggacgtatgc catcagctgt tggttatcag ccaacattag ctactgaaat gggtgcactt
    35281 caagagagaa taacatcaac taagaaaggt tcaataacat ctgttcaagc agtatatgta
    35341 ccagcagatg acttaactga cccagcacca gctacaacat tctctcactt agatgcaaaa
    35401 acagttttat caagacaaat atcatcactg ggtatatatc cagcagttga ccctcttgaa
    35461 tctacatcaa ggattcttga ccctagtata gtaggaaaag aacattatga agtagccaga
    35521 ggtgtacaat caatacttca aagatataaa gaacttcaag atataattgc tatacttggt
    35581 atggatgaat tatctgatga agataagtta atagttgcac gtgctagaaa gatacaaaga
    35641 ttcttatctc aatcattcac agttgctgag caatttacag gaaatccagg tcaatatgtt
    35701 cctgttaaag agactgtaag aggatttaaa gaaattcttg aagggaaaca tgatgactta
    35761 ccagagtcag cattcttatt tgtaggaaca atagaagacg ctgttagaaa agcgaaggga
    35821 agtatgtagt catggccagt gaattttcat tgaaagttgt aactccaagt aatacttttt
    35881 atgatggaga tgttgaaatg atagtagtta gaactacaaa aggagacaaa ggggtgttga
    35941 aaggtcatat accatttgtt tcaggtatac ctgtaggaac tttaaagata aaaaaagagg
    36001 gaagttttaa agaggcgaaa atagcagaag gattcattaa tgtaactcaa gataaaactg
    36061 tcatattgac agaatctgct gagtggattt aataaattaa tttagagtat aaaaagtcat
    36121 tttgaccaaa aaaacttttg ggtaaaatgg cttttttgcg ttttggtgtt aataaaataa
    36181 cgatgaaatt gtaagttttt agtgataata aattcacaaa atgtttaaat ttactgccaa
    36241 attatgttgt aattaataag ataaagaaga tttagatagc ttttatgtaa gtgaaaaagt
    36301 cttcatataa gaaatacata taaaattttt ataaaattta aaaaatttgc ttagttgtgt
    36361 agtataataa ataatataat agatttgtat ataatattca ttacaaataa ttttaaaagt
    36421 tacagtttta ttataatgat tattaaatta agataagtaa cataaactaa ataataatgt
    36481 gtattatcga tagtaaattt ataaattcat tattaattat tgaaagaagt attataaatt
    36541 ttctggagga acaatgaata tatttgatat tggcgtagat ataattgaaa ttgatagaat
    36601 aagaaaggct gtagacaaaa ataataggtt tttggagaag atatttacag atagagaaat
    36661 agagtatttt aattctaaga attttaaagc agaaagtata gcaggtaatt tcgcagccaa
    36721 ggaagctata agtaaatcta taggtacagg aattagagtg tttaatttta aagatataga
    36781 agttctcaga aatgaaatgg gaaaaccaat agtaaaaact tataataatc tagcaaaaat
    36841 gtgcatagat tataatgttt tagaaataaa agtttcaata tcacattcaa aagattatgc
    36901 tatagcaaat gcgataacca taattaagga ctaaggagtg attacattat gatggataaa
    36961 acagcaaaag aaataatgac aacagatgta atagtagcaa aacaagatga tagtatagca
    37021 gatgtagcta atatgctaat tgcagaaaaa ataggaggtc tgccagttgt agattcagag
    37081 aatagagttg ttggaataat ttctgaaaca gatattctta aaaaagaaaa atatatagaa
    37141 gcacctctct atataaatct attacaagga ttgattttct tagatgactt aaaaaaagtt
    37201 gaaaaagata taaaacaagt agctgcttat aaggttggag aacttatgtc taaagatata
    37261 ataaaggttc atgaagatga taagtttgat gatgtagcta atgtaatgat taaaaaatca
    37321 ataaacagag ttcctgtagt agatgatgac aacaaactta aaggtataat ttgtagatat
    37381 gatataataa aagctttata caatgaataa actgaagtag gaggtgtgat gtgagaaaaa
    37441 agtggaccat agtatgtatt atgttcttgg cactggttat aattgtaata ggatgccaaa
    37501 aacgacagtc cacaaaagaa gaagtataca aagattttca aaagcagatt tctgatatga
    37561 attattacag ttgtaaagct gaagtagaag tagttggaaa taaaagccca cacaattatg
    37621 ttttaattca tacttataaa aaaaccgata actataaatt agaagtaatt tccccaaaac
    37681 atttaaaagg gaaaagtata gaatatcaag gtgataaaat attagttaaa aatccaaaga
    37741 ttagtgatgt agtggagtta cctaatacag gaaaaaataa tcaatatctt tttgtaggag
    37801 actttataaa aaattatctt caaaatgagg agatgaaagt aaagttatcc aaaggacatt
    37861 tggttttaga gacttttatt cctggggata ataaatattt taacaagcaa gtattatatg
    37921 taaatgctga cacaaaaaat cctgaaaaaa tggaagtgtt agataaagag ggagtgccaa
    37981 gatttacagt aaaatacaaa gattttgaat acagaaacta agggggacta aagacatgca
    38041 aaaaataaca gtgcctacat gggcagagat aaatctagat aacttaagat ttaacttaaa
    38101 taatattaaa aatttattag aagaagatat taagatttgt ggagtaataa aagctgatgc
    38161 atatggacat ggtgcagtag aagttgcaaa attgctagaa aaagaaaaag tagattactt
    38221 agcagtagca agaactgctg aaggaattga acttagacaa aatggcataa cacttcctat
    38281 tttgaacttg ggatatactc cagacgaagc ttttgaagat tctataaaaa ataaaataac
    38341 tatgacagtt tattctttag aaacagcaca aaagataaat gaaattgcaa aatctttagg
    38401 agaaaaagcc tgtgttcatg ttaaaataga ctcagggatg actagaatag gtttccaacc
    38461 taatgaggag tcagtacagg aaataataga attaaataaa ttagaatata tagatttaga
    38521 aggtatgttt actcattttg ctacagctga tgaagtaagt aaagagtaca cttataaaca
    38581 agctaataat tataaattta tgtctgataa attagatgag gctggtgtaa aaatagctgt
    38641 aaaacatgta tcaaacagtg cagctattat ggattgccct gatttaagat taaatatggt
    38701 aagagcagga ataatattat atggtcatta tccatctgat gatgtattta aagatagatt
    38761 agaattaaga ccagccatga aattaaaatc aaaaatcgga catataaaac aagttgaacc
    38821 aggtgtagga ataagttatg gactaaaata cacaactaca ggtaaagaaa caatagctac
    38881 agttccaata ggatacgcag atggatttac tagaatccaa aaaaatccaa aggttcttat
    38941 taagggagaa gtgtttgatg tagttggtag aatatgtatg gatcaaataa tggttagaat
    39001 tgacaaagat atagacataa aagttggaga tgaggttata ctatttggag aaggcgaagt
    39061 tacagctgag cgtatagcta aagacttagg aactataaac tatgaagtgt tatgtatgat
    39121 atcaagaaga gttgaccgtg tttatatgga aaataatgag cttgtacaaa taaacagtta
    39181 tttgctaaaa taaaaccatg atattataat ctgggaggcg attttgtgta cgataagagt
    39241 aaagaaaaaa tagaagttac tttacctgac tctctgattt ctgaagttga tagtatagtt
    39301 cagatggaga acagtaacag agaagatttt gcaaaagctg cttttcagtt ctatataact
    39361 cagaaaaaga aaattcatat taaagaatct atgaaaacgg gttatagaga aatgggtcaa
    39421 attaatctgt cactagctga attaggtatg acagttgaag aagtatcttc tgatgataaa
    39481 aatgaaggga agattgccca aggtgagtac taattgtgaa ataaagcgtg gtgatttata
    39541 ttacgctgat ttaagtccag ttgttggttc agagcaggga ggcgttagac ctgttctgat
    39601 aatacaaaat gatgtaggta ataaatatag tcctacagtt atagtatcag ctataacatc
    39661 tcagattaat aaagccaaat tacctactca tgtagagata agttccaatg aatatgggct
    39721 taataaagac tcagtaattc ttttagagca aattagaact atcgataaaa agagactgag
    39781 ggaaaaaatt ggttgtctag atgaaaatat gatgataaaa gtagatgatg gacttcaaat
    39841 aagtttagga ttgtttactt tttaaagagg gatttgtctt ttattagaca atcccttttt
    39901 tcttttaaat atctcttaag gtttaatttt aatgtactat gttaataaaa ttaaaaataa
    39961 cgcaaaaaaa tagcataaaa gtgatataat aatatggaat tgatataaaa ttacgaactc
    40021 aagggaggta attgcatgag agaccataaa ggattaaatg agattgcgcg tatcataaga
    40081 agagatatag tttcaatgat acatagagcc aaatctggac atccaggagg gtcattatca
    40141 gtagttgaaa tacttactgc gttatacttt gatgaaatga atgtggattc ttcaaatcct
    40201 aaaatggagg atagagatag atttgtatta tcaaaaggac atgctgcacc agcattatat
    40261 gctacattgg ctgaaaaagg ctattttgat aaggaagaaa tgaatggatt aaggaagata
    40321 ggaaggatgt tacaaggtca tccagacatg aaaggcacgc caggtgtgga aatatctact
    40381 ggttctttag gtcaaggttt ttcagttgca tgtggtatgg ctatggcatc aaagttggat
    40441 aatgctccgt ggaatgtgta tactttgctt ggtgatggtg aggttcaaga aggaattgtt
    40501 tgggaagctg ctatgagtgc tgcacattat aaacttgata atttaattgc ttttttagat
    40561 aataatggac ttcaaataga tggagatata gaaagcgtaa tgagtttagg ttcaatagtt
    40621 gataaattta aagcttttgg ttggaatgta attgagatag atggacatga ttttgaccaa
    40681 atatttgcgg ctcttgatat agctaaatca acagttaaaa aacctacaat gatagttgca
    40741 aaaactataa aaggcaaagg aatatctttt atggaaaatc aagcaggatg gcatgggaca
    40801 gctccaagtg atgaggagct agaaaaagcc ttgcttgaat taggaggtgt tgacaatgag
    40861 taaaatggcg acgagagaag catatggtaa ggcattagta aaattaggtc aaataaatga
    40921 caatgttgta gtattagatg cagatttatc aaagtctaca aaaacacatg atttttacaa
    40981 gacatttcca gatagatttt ttaatatggg tatagctgaa caaaatttaa taggcgctgc
    41041 ttgtggatta tcaactgctg gtaaaatacc ttttgcaagt actttcgcta tgtttgctac
    41101 gggaagggcc tttgagatta taagaaattc agtttgttat ccaaagctaa atgtaaaaat
    41161 atgtgctact catgcaggtc ttactgttgg agaggatgga gcatctcatg aaagtgtaga
    41221 agatattgct ataatgagag ctataccaaa tatgacagtt cttgttccag ctgatggagt
    41281 tgaaacagaa aaaattattt ttgaaatagc taagtataat ggacctgttt atgttagatt
    41341 agggagaagt tcagtaccag ttttatttga tgaagattat aaatttgaaa taggaaaagg
    41401 tacagtctta agagaaggaa aggatgtatc aattatagct tgtggaatta tggtaaatga
    41461 agctctatta gcacaagaaa agttgcaaga agaaggtata agtgctagag ttataaacat
    41521 gtcctcgata aaacctatag acaaagattt aatattagaa tcagcaaaag aaacgaatgc
    41581 tattgtaact gtagaagaac atagcataat tggtggatta ggttctgctg taagtgaggt
    41641 agtgggagag tcttgtccaa ccattgtaaa gaaagttgga attaaagata catttggaga
    41701 atctggtacc ccaaatgaat tgttaaaaaa atatgaatta acttgtgatg acatagtaaa
    41761 gactgttaaa gaagctatta ttgcaaaaag aatgtaagag atataaatta ttcatgtttg
    41821 ttaataatat aatatattta aaagagaggt ataaagtgtt atattaattt atatctctct
    41881 tttttttata tgatatcaat atatcaaaaa tataataaaa tattcaaatt tttacaaaaa
    41941 tagagaaaat attcaaatat atattgacta ttcagaaaaa tatgactaat atataattta
    42001 tagagaaatt aattttatat ataatgacta cttagataaa ataatctaaa aaaattatga
    42061 aaggaggact agggtataaa attagattat aagttttaag ttattatact aaaaagctat
    42121 aaaacagtta tcatgtattt tatgttaaag gaaattgtag gtagtttatc gaattgttta
    42181 agcagaagaa aaaatttcac atggggaggt aactgagata tgtcagaaaa agtaaatgtt
    42241 aaaacagtca tcagtttcgc aggtgcgtat gtagcaacag taatcggttc tggatttgca
    42301 acaggacagg aaataatgca attcttctct ttttatggtt ttgcaggtat tattggtggg
    42361 ataatttcaa tggtattatt ctcatggatg ggtgcttcag ttatgtcaaa aggtaaagag
    42421 cttcaactta aagaacctat caaaatttat cgtgtttatt gtggtaagta tttaggaata
    42481 ttctttgaat ggtttggacc tttattctta tttggtgttt tcgtagtaat gatttcagga
    42541 gcaggagcaa ctttaactga atactatgga ttaaatccat ttgtaggtag agtaggtatg
    42601 gcagtagtag cattattaac tgtttcatta ggtcttgata gattatcaaa gatactaggt
    42661 ggaataggac ctataataat aatatttaca cttttagttg gtggtataag tttagctaaa
    42721 aatataggta atataggaga agctgcacaa gttcttagca ctgtaaagat agctaaacca
    42781 gttccaaatg cctatctatc tggtgtaata tatactactt ataacacaat tgttgttatg
    42841 gcattcttaa ctggattagg agcaagtgct gctaacaaaa aagaagctat ttgtggtggt
    42901 atattaggtg gagtagcact tatggctgca gctataatga tgcacttagc tattctttct
    42961 gatataggaa acttatattc aaaagcaata ccatcattat tcttagcaga taagatttca
    43021 ccaataattg gagttttatt ctcaataatt cttatattag gtatatatac aacagctgta
    43081 cctttattat ggtcagtaac taatagattt gtagatgata atcatcctaa gtttaaatta
    43141 ataactttaa tagcagctat attaggtctt ataggtggat tcctaccatt tgataagctg
    43201 gttggtattt tatatccata tactggatat atgggcgtca taatactggt ttgtgttctt
    43261 tatagacaga ttacaaaaac atctggatat caagaaggaa tcagtgacaa ataaatattt
    43321 tttaatattg taaactcaag agctatatga aaacaatgaa aatgttttta tatagctctt
    43381 tttattttta gcttgtttat tatgaaatag aaaaaacatg ttttttagtt acattacata
    43441 tatttataat tatgtataaa tatatactaa tcaataattt agagtctaag ataagtttat
    43501 aaatcatata tttagaatat atgggaggag tgataagatt gaatgtaaag tttaatatta
    43561 aagggataat ttatggagtt atagctcttg tattcgttat agggggattt atattcatac
    43621 caagtatgtt tgtagagaaa tctaaaccca tagattatac agtattacaa agaaattcaa
    43681 taccagaaaa gatactagat gtgatggata agtatacaaa tgaagaaaga gcattagcgg
    43741 ctaaaatcga taacaaaata tacattatag tgactagagg aaataataaa tatggtatag
    43801 aaatggacaa aatagaaagt gtggttgaag aaggaaagga tgttttgagg gtcaagataa
    43861 agtataaaga taaggaagat tcttttccat acatagttgt tgaaacaaat atgagtgaac
    43921 ttccagatag aatagaatta aatgcaacga aataagaagg tattttataa ttaaataact
    43981 gcatattgta caataaagtc atggttttta ttaatgtaag taaattgtat atggttctta
    44041 ttgttgtaaa agccatgact taaaatgctt ataaagtaag atattaacag tagtatttat
    44101 tactacatta aatggggtct ggaaatactt aatattctaa ctatttggag tgataattct
    44161 agtttcagta actatgtagt ctaattgtgc atcatgagtt tcttttggaa tagaatcgaa
    44221 tatctggaag tcaaatgcta tgccgatagt aatagcatct tttcttaagt tttctaaaaa
    44281 tctatcataa aatcctccac catatcctaa tctatagcaa tgtatatcat atgctacagc
    44341 tggtacaatt acaacatcta aggccttaat atcaactgta ggtgattttt cgttaggttc
    44401 tcttattcca tattctccaa ttctcaatcc ctcttttaaa tctgttattt gagtagggat
    44461 taatgtatga ttttctttta ttgtgatggg acttgatact attttcttta gagacaaaag
    44521 ttttttgatt aaattatctg tttgaacttc attgttaaaa tttagataaa gcattatatt
    44581 agttgcattt cttatatctt ccatttgaag taatttttca gtgattaaat ttgaattact
    44641 aagtacgaag tttgcactct tattttttct tgattcaata actttttttc taaattcttt
    44701 tttcataaaa taattcctcc cttaatatgt tataatgtgt acagcaattg tatttaatat
    44761 aaattttagt ataatttatt gtaataaagc aatatatttg ggtaaatgta ataaaaataa
    44821 cagctagtca tataataata atagtttatg taaaaggaga tgtatcttgt gatttcaaag
    44881 aagaaagcta tttttttagg ggtgatttta gtaattataa cagctatggt tacatcagca
    44941 tttcagttga cattggggaa taaagttgta atatcgaaag aactctatga agactataaa
    45001 aaatatgata agttgttagg actagaatct atcataaaac aagattttta taaaaaagta
    45061 tcagatactg atttggtaaa tggagcatca aaaggtctat ttctaggaac aaatgataag
    45121 tattctggtt attatactaa agatgaaatg gagaatctaa ttaatgatag tgaaggttct
    45181 tatgttggtg taggtatgta tataggggca tcaaaagatg gtggcttagt agtagttccc
    45241 atgaaagatt ctccagcaga gaaagcaggt gtaaaatcag gggataaatt agtaaaagtc
    45301 aatggaaaat ctgtctcata taaaaattca gatgaagctg ttcgtatgat gaagggtaaa
    45361 aaaggtaaaa cagttgaact gactatatta agagaagata aacaattgaa ctttaaagta
    45421 aaaacagacc agataattga gaagagtata gaaagtaaag taattgataa tgatttagga
    45481 tatatagaaa ttacccaatt tatatcaagt acatatactg actttgataa agctttaaaa
    45541 gaattaaaag caaaaaatat caagggatta gtaatagacc ttagaaataa tcctggtgga
    45601 atgctagata tatgtaaaga agttgctgat gagttaattg gtgaaggaac tatagtatat
    45661 acaaaagata ataagggaaa tactgagtac ttgaaatctg ataaagaaaa attgggactt
    45721 ccaatagtag tgttaactaa tggagaaagt gcttctgctg ctgaaatatt aacagctgca
    45781 atcgttgata ataaagaagg tatttctgta gggactacaa cttttggtaa agggttggta
    45841 caatcagttg taagattgaa ggatggaaca ggatacaagc taactactgc tcaatatttt
    45901 acacctaatg gagattatat caatgaaaaa ggtataaagc ctactattga agagaaagat
    45961 gaaaataaac agttagatgt agcaactaag tggcttagag aacagattga taaataggat
    46021 ttaattttta taaacttata ctataaaata aagaacatta taagagagat gtctcaaaat
    46081 gaacttttta gttcatgagg gagtctggaa atatttatgt gtattcttta aataaaaatt
    46141 tattttttaa gtgataatta aatttttaca gacagggcta tggttttaaa aatcatagcc
    46201 ttattttgat attttttaaa aacacattgc aacccagttg ttccaaatat tatatcattt
    46261 acttttaaaa tatcgcaaac tgcatattat atattcatta taatataatt tatctaacta
    46321 gaaccttagt ttactaatta aattagatgc atagaataac ttttttaaac attgaaaata
    46381 aattaaactc taaacacatt taaatataaa tcacaaagta atttttgatt atcttttcaa
    46441 aagttcttat atgccggtat agcctattgt tacaggcttt tccggcattt ttcgttatgg
    46501 aagatattct caaatcttct tatatccccg tatgttttgg tgttcaaaag tagtatgtgg
    46561 agtagtaaat tctccattta ctactatgca atcagccgct tcatttctgt tattgacgtt
    46621 tcggaagttg tgtgtgcgta atagttcagc gtcatattga tgttggaatg tcccatgatg
    46681 tactgcaatg cttttgggtt cattcccgca tttgctaacg tggtgcagaa tgtgtggcgt
    46741 aaggtgtgcg gtgtcattac ctttggcaat gctacctttt gcgttttgtt gtatttcctt
    46801 acaagccccc ggaacatact ttcatagttg actgccactt ttggcaaacc gttccggtta
    46861 aggaacagaa actttgtgta gccctctaaa ataacaggct gtgcatattt ccgcttgttc
    46921 agcacccgct gaaatgcttc taacactttt tcactcatag gaataacccg gttgccgctt
    46981 ttcgtcttag gcgtttccac atagtagccc acgtctgcaa tcttcaaaag ttggtggtct
    47041 acattgataa gctgtttgtc taagtcaatg tcagcttccg tcagtccgca gagttccgaa
    47101 atgcgaagcc ctgtccctaa cagtatgata atctcgtcgt agtatttctg atagacttta
    47161 tcatgctgca caaaggacag aaaagccgct tcctgcgtag gggatagagg ttcctttggt
    47221 tccgtatcat cttcaaggac tgtgttcaac tggaagtcaa atggattttt acgaatacaa
    47281 tcgtcctgta tggctgtgta aaaggcagcc tttaaggaac gcttgtggtt gttgatagtc
    47341 ttgaagccat aacctttttc tttcatgcgt aacgcccatt cctttgcgtc ggagagcttc
    47401 acattttcaa tccggcaggc tccaagttta tcctcctgca aaatccgcat gagctgtttt
    47461 cgcccctgct ttgtaccatg ccgcacattt gcccggtggc gtatctgctt tgcgtagagt
    47521 tggcagaccg tcattttctt tccgatatgg tcgataccgt cgtcaaggtc tttttggatt
    47581 tctttctctt tttccctcaa cgctatatcg tcacgctttc ctgccggagt tttgtccgta
    47641 ggtactaact tccaagaata aacaaactgc ggttttccaa aggtatctat atatttgtag
    47701 gcatatcttc cgtcttttct ctggctctct ccggtgcgga gaatacgatt tttattatcc
    47761 cgtctttttt ccgacatcgt tatgctcctt tccataatgg aaagagcctt gatatgcttg
    47821 attacatttt accacattca aggctcaatt tcactatcag attgcgtcca ccgtatcaat
    47881 cattttttca aactgcttac gcttaatctg aatacggttt ccgttcatca ttacccagtc
    47941 cgggacggga ttttcttctg ccagtttccg cagcttgctt tctccgatac ggaaatattt
    48001 tgcagcttct tctatggtaa gggtgtattt ttcccaaatc ggcacgtcgt tattgttcaa
    48061 tttgtcgtcc ccctttctaa atgggtcaaa agtcaatctg taacataggc tttaatcgga
    48121 cggctattag cgtaacctca tgggacttgc acccctccgg cggtctgccg aagccctacc
    48181 cattgcctgc gacgcttcta acgctcggac tatggctgta aggaagtatc attatgtatt
    48241 ctgtgcgtta tcgcccgcag gctgctatct ctgctttacg gtgtggtatt tctcgctcac
    48301 ttttctatta agagaaaggt catggcgtac ccgccgcatg gctcggcaca aaaggaacgt
    48361 atccgatttg ccttatactg cgtcgccgtt ctattgcgcc gctttcttta tcaaagagca
    48421 gagggctgtt ctgcgatatg gaacggaaaa gggggaagcg ttccaatacc gcattggttt
    48481 attcagccct aaatgtgagg attgccctca ttagttttgc ttgcaacctt tcgcgtattt
    48541 cttcgtctac tccaaaataa acatttcctc gctcatcata gagcttccgc atggaaagac
    48601 ttgctatgta gccgctgaaa tgctgcaata caatcttcat tgcgtccggg tcgcctttgc
    48661 tcgctgccaa aataacggga tagggaacaa gggcattttc cgggtagtcg ttttgattac
    48721 cattcaatcc attcatcagc gttttcctcc aaatattttt ttaactgctc aaaggagctt
    48781 gtccggcgat actgtaccgt acttcttgag agattgagct gctttccgat ttctatatca
    48841 gttaatccct caaagtaata cagcagtaca attttgcgcc gttcttcggg taaagcccgg
    48901 atagcttcaa gcagcagttt tggggttatt tcttttcctg ccatttgata agacggctct
    48961 acttcttcgt cttgaaaata cttatcgacg gtataaagct gccgctcctc atgcaaggca
    49021 aggtcagaaa aagcaacttc ccgaactctg cgccgacgaa tttcttcatg ggcgttgcac
    49081 gcttcattgt gcagcactga tttacagaac ttttgaaacg cacattgctt ttcaaattcc
    49141 ctgcgattag gtgtcatggt gttcacctcc tttctgctga aaggcggtag tacctcccct
    49201 tttcgcggga taatacagac gactttttgc tttgccaaaa agagagcaaa gtttttgaaa
    49261 aatcttctgt aaaatgcaaa atgcgcctgc ctgcaaagtg cagacagacg cataggaaat
    49321 aacagcagta tttaattgat ttgacagttc ataccactgc ccggaatatc ccgttgtaag
    49381 gtacaaactt tttttgacaa aagactttct tttgccatat aagcaaaagc tattttactc
    49441 agcataacgg caacctccta tatgccgcca tgctgattgc aaggtcaaat ggggtcgtcg
    49501 ttgctaaatt tatgcaaaaa gaaacggcgg ctctaaaatc aaagccaccg ctataaaggc
    49561 gcgtgaaaat atatctgcat tacgacggct ttttttcttt tgccgtcgtc cggcaatcat
    49621 tagctttcga tttttttctt tcgggtaaaa ataaatattc ccataaaaag cattagtcca
    49681 aatgaaatta tactgctaat gcagccaccg attgttgcct gttttgccaa ttcataatat
    49741 gtagaactat ccgaaaaata catagataaa cgataggcaa aactccccgg aataacatat
    49801 ccaaattcac ttccaataaa gaagcaggac acaaatccat aaattacgcc tataacactc
    49861 gttacaatga tatttctgct tatattgatg ataattgctg ctatgcaagt tgtaatcaga
    49921 ataacaatta tagcttctaa tgcaaatcct acggttaagg ataaaatttc ttgaatatca
    49981 acagaaactt ttcctgcgat tttcagcatt aaaatcaaaa tatgaaacag gcatattagc
    50041 gaggcggcaa atacaatctc aataacaaat ccgacgataa tttttgcact gaataactgt
    50101 tgttttgaat atccgtaagt aatccagttt atataggttt tatttttata ttcttgataa
    50161 aaaagcgaac ctgtcaaaat aacaattacc atagggaaaa acatggaaaa ttgattattc
    50221 ataaagaaaa ataaatcact tacggttttt gtactattgt taaagacagc ccccactccg
    50281 ccagttaaaa caggtagcca tgaaaggaga agtaaaaata atacaaacca ttcacgtttc
    50341 aatttagtaa attcgttctt tattaaaact ttcattgtac ttcctccgta gttatggaag
    50401 tcataagatt ttcataaaga acttccaatt ctttctcatc atatttgttt tcaatcattt
    50461 ctataatctg cccattacgg ataaagataa gtatatctgc gattgccgca atttctgtga
    50521 gattatggct tgatacaata atacagtgct ttggggtttt taatttatca cgtaataata
    50581 tccgaatgtc ctttatcccc atagggtcaa gcccatttgt tggctcatct aatagtaagt
    50641 atttcgcatt tcctaaaaaa gtgcgggcaa tcccaagtcg ctgtctcatg ccaagagaaa
    50701 gctgtgaaaa gagcttattt cttgcttccc ataaattgac ttgtttcaaa gaattttcta
    50761 agtcgggatt tgtaagccca atataacttg catgaagttt cagattttcc atgacggaaa
    50821 gttttgggta aaatgcagga tattcaatta aacttccaaa ctcactactt ttagcgggat
    50881 ttttatctga aagaatttcg cctgtataag cagtcagtcc taacatagac ttaaacaatg
    50941 tggttttgcc tgcgccattt gcaccaagta gtccatatat ctttccttgt tctaaagaaa
    51001 gattgatatt gtgaagtagc ttgttgcttc cgacacaaag atttacattt ttaatttgta
    51061 acattgtaga cctccattat tgtgagatta taaaagtcaa aaaagttatt gcataaatac
    51121 aaaatgtaac accaagtagt tttccttttt tttgcaagcc tttcataccg tcgcttaaaa
    51181 taccactgat tgctgcaata aaacatacag caagtgcaac aatcgaaatg attaaggaaa
    51241 ttttagataa catttgacca cctcccttgt aatgtatagt aacaaagaga gttatgattt
    51301 ttttaggaaa agtctaagaa aagtctaaag ataaaaacag ggtagccata gccaccctgt
    51361 ttttataaaa ttgtaaagga aatacatatt tcaaatattt cattgtgtaa atgtgcagat
    51421 atttctccac ccatttgtgt aatcagttta cgcgcgattg ctaaccccag tcccgttgtt
    51481 ttttttgtac gagaactgtc tgttgtatag aaacggtcaa acagatgagg tatatcctct
    51541 ttacagattg tatttgaatg gttggaaata ctgatataat aacactttct ttcaactgtt
    51601 gcatggattg tataaacact atcgccatgt attaaagcgt tatatatgat atttgaaaaa
    51661 accctaaata atgcgtcttt gtctgcccaa atcatagccg gactatcggg aatatcaata
    51721 gtaggggtaa tagcttttat ttcaaagtca ttatagtatg ttgctaaggc atctcttaaa
    51781 acactgttgg catctactaa ctgtttattt agttttagtt cattcgcttc aattctcgca
    51841 aattcaaaaa gctggtcaag caatatcttt actgccgaaa ctcgctcatt tgctattttt
    51901 acatattctt tctgttcagc ggatagaggt tgttctaaaa gtagttgtat atagccattc
    51961 gcagttgcga gcggggtacg caaatcgtgg gaaaggcttg tgattgtatc acgaaataaa
    52021 ctgtcatttc gttcaatttt tatcccggtc aatctatatt ttttcaaaag atgattgata
    52081 ctaatagcaa gttcttctgt ctgtttatcc agtttttcta aggttatttc tgcttgtgta
    52141 tctctggaac ataaaaagtc tatttgattt ttgattttag ctatttgttg cttttgaata
    52201 attactttta atagtaatat caaagatgta actacaagaa aaatacaaaa tatgctcata
    52261 ttatttatcc ctttcgtgct tgcgcaaatc tatttttttg taaataacga atgaacataa
    52321 tgtagggaaa aacacatatc cggtaatggt aagcaatatg agcggtaatt gtggcagtac
    52381 taatctgatt tccattgcat gagataaccc tgctatccaa taacgatata gtgaatagga
    52441 taagtctaag gctgaactta tttttgtaag atagaagtat aatgtcccca ataaaaaaat
    52501 agaaattgtg ataacaaggc tacgataacg aaataagata cttagtagcc aaataaaaga
    52561 agtatagcct ataaagcata aaatctgtaa aaacaggtag ataccgatta gcccaaacga
    52621 gcaatcaatg taggattgtt ttacccacat aactaagata aaaatatatg ccataagaaa
    52681 attgaacagc atatacagga cagtggcaat ccatgaagaa attagtttac caaaaaaaat
    52741 tgatgttctt gtaattcctt ttgacaagaa aatcgaacaa gtaccagtcc ggctgttttc
    52801 tgtgaagtag cagcccaaga ataccgtaat aggaagtatt aaactataat ctgaaaacaa
    52861 aaattgcgta taatcgttta aggacgcttt cggaacagcc gcaacggagt gctttattgt
    52921 attgtaatga aaagtcaatg caagtgcaaa aacaaaaaat gtggatacta tcaaacatat
    52981 cttaaataaa cgatttttgc gaatacgaaa aagctctcct tgtatcattt taatcatagg
    53041 ctatattcct ccttccgtta attttagaag atactcttgt ggcatagttc cagcaatcca
    53101 aatttctgat acctctattc cggcggatac taataatgta tttatcttag cagaatgaga
    53161 taaggggcaa aatactctga ttaaatcatc ttctaaaacc tcatattgcg ggaactcttt
    53221 ttctaataaa ggaattgcag aaagtaattg tggcgttctt attttgatac agcgtttgca
    53281 ctcactgtct aatttttctg gagtgagttc tattaagaaa cgcccatttt taagaactcc
    53341 atattttgtt gcaattcggg aagcaagctc ataattatgt cctgtcaaca aaaaagtgat
    53401 attattattc ttattcagat aagatatgat tgaaataagt tgttcacttt cttgagtatc
    53461 gagtcctcca aatgggtcgt ctataaaaac caaatcagga tttcccaaaa gagcaatcca
    53521 caaatttagt tttcgttgga taaaggaaga taaatgacga acaggtttct tttctttcag
    53581 attaaggttt aatatatctg caaagttttt ttctaatacc ccaaatgata aagaaaaata
    53641 attcatcata tctgttatag tcattctgtc aatgtaatat ggactttgtg gtacatatcc
    53701 aatacgcctt ttctcttgta aaaaatcggg attttcaaaa attttaatgc ttcccttttg
    53761 tggagaaatt ataccaagta ttgattgtaa tattaaactt ttgccggagt tatgacttcc
    53821 atataaataa taaatatcgt tataatctat ttgaaagtct atgccaataa taccaacgcc
    53881 atttgggaaa acataggata aatttttcac ttctaaaaca agccccacac ttccacctct
    53941 ttctattatc gtatatgtat tgtgaattaa agattatagc tttatttata agcattttct
    54001 aaggattact ctgcatttta aagcccaatc cccatactgt ctgtatatac tgctcattcg
    54061 gatttatctt agcaaatttt ttccgaagat tgcttatgtg gacattgatt gcattatcat
    54121 ctccgcaaaa agtttcattc catacacttt caaagatatt atttttggta aagaccttat
    54181 taggggcagt catcaataac tgtaaaatta aatattcata tcttgtgaga acaaggggaa
    54241 tatcttttat tgtcacttcc attgttgccg gattaagcat taagtcctta aattttaata
    54301 gggagttatt gctgcttcta tgacagcgac gtaaaatagc ttccattcgc actaacagtt
    54361 ctttattatc aaaaggcttc gtaatataat catcagcacc gttttttatc aaatcaattc
    54421 tactgtcaat atccgttttt gcagacacac caataatcgg cgtagtttgg ttttttctta
    54481 ttgttgtcaa tacttcttcg ccagatattc ccggcagcat taaatcaagt aaaattaaat
    54541 caaaagtttc tttttcaagg attaaaagtg cttcactccc cgaataagct gtattaactc
    54601 tatatccgtt ttgagataat agacttttta gcatatcact tagtaaaata tcatcttcaa
    54661 cgattagaat ttgtagtatc tgctgcatgc tgtttcctcc tgtcaatcgg tttttctgct
    54721 ttttttggta tcagccatat acggctgaat ttagtcgctc cgggtattct gttttcttca
    54781 cacaactttt gtactcgtct ttcagaaatt ccccaatttt ctgcggcttc catgcaagat
    54841 atatactcca tattttgaca ccctttcatc agaaattaac ttcgccaata ttattatata
    54901 cgttcaaccg aacaaataca agaagtggcg cataaataat atgctctatt ttagtcaata
    54961 ctacgatatg tgtaatcata tctcaaagaa aagaggaaca gtaaaatgta actgggtgaa
    55021 ataattatga agcagagtaa agagaaattt gattttaagg ctttcggaca agccattaaa
    55081 gcagcaagaa aagcaaaggg aatatcacgc aatcagttag ccgacacgct gaatattgcg
    55141 ccccgatata tcgcgtccat tgagaacagc gggcagcacc caagtttaca gattttgtat
    55201 gagcttgtga ctttgcttga cgtatcagta gaccagttct ttttcccgga acgagaacag
    55261 gagaagtcca cacgccgccg tcagctcgat accatgcttg acacaatgag cgaaacagac
    55321 ttaaaaatca tgtctgctac tgcaaaaggc atagaggaag ccaacaacga caggacgggg
    55381 gaataaagtt ccctcgtttt tgtctgcata aagtgattga aacgtcgcag gattgcggcg
    55441 tttttctttt gctctttttt ggcatactgg gagtttcgtt gtagtgataa gtgaggaaaa
    55501 aactttctta gcaagcatag gaaaagagta cccttttccg tattttcgcc ctcccgtcct
    55561 gcggtgcgtc ggttgaaaat tgcttgttgg gaagtgtccc aaaccctcgg aatggaaagg
    55621 agcgattgca tgaacggacg caaaagaaaa atacaaatca aattctatgt aacagaggaa
    55681 gaacgggcat tgattgagca gaaaatgaag ctcgttccca cccggaacat ggcggcgtat
    55741 cttcgcaaga ttgccattga cgggtatatc attcagacag accatagcga cataaaagcc
    55801 atgacagcgg agatacagaa aatcggggtc aacatcaatc agattgcaag gcgtgtcaat
    55861 gcgacaggca gcgtctacca agaggacata gaggaaataa agggggtgct tgcagagata
    55921 tggcggttac aaagattaag cctattaaaa gcacgttgag caaagccctt gactatatcc
    55981 aaaacccgga taaaacagac gggaaaatgc ttgtgtcctc ctttgggtgc agctatgaaa
    56041 cggcagacat tgagttcgga tttacgcttg cacaagccat agagaaggga aacaatctcg
    56101 cccaccattt gatacagtcc tttgagccgg gggaagtgga ttatgagaaa gcccacgaaa
    56161 tcggaaaaca gcttgccgac gcggtaacaa agggacagca tgaatacgtc ttgactaccc
    56221 acatagacaa agggcatatc cataaccata tcattttttg tgcggtcaat tttgttgact
    56281 accataagta caattccaac aaacgcagtt actacggcat acggaacatg agcgataagc
    56341 tgtgccggga aaacgggctg tctgttgtcg ttccgggcaa aggcagcaag ggaaaaagct
    56401 atgtagaata tcaagcggaa aaggtcggta caagttggaa aggcaagctg aaaattgcca
    56461 ttgatacgct tatcccccaa gtagcaagtt ttgaagaatt gttgcagcgg ttacaggcag
    56521 cgggttatga gataaagacg ggaaaatata tctcctgccg cgctccagga caggaacgct
    56581 ttacccgtct gaaaaccctc ggtgcagatt atacagagga agcaatcaaa gaacggatag
    56641 agggcaaacg cacaaaggcg gcgaaaactc ccaaagagca gcgcggcgta tcgctgctta
    56701 ttgacattga aaacagtatc aaggcacaag aaagccgggg ctatgaacag tgggcgaaaa
    56761 tccataatct gaaactggct gcaaagacta tgaatttctt gacggaacat aagattgaac
    56821 agtacgcaga tttagtcagc cggattgaag aaataaatgc ggaaaatgaa aagacagcag
    56881 acgcattaaa gagcgtggaa aaacgtcttg ctgatatggc ggtgctgatg aagcacgtta
    56941 ccacctacca aaagacaaaa cccgtctatg aagcataccg cagggcaaag gataaggacg
    57001 cataccgggc aaagcaggaa agcagtctga tactccatga agcggcggca aaggcactga
    57061 aagcgtcggg cattaccaag ctcccgaacc ttgccgccat gcaggaagaa tacgggaagc
    57121 tccaagcgca gaaagaagcc ctttacgctg attatggaaa gctgaaaaaa caggtcaagg
    57181 aatacgacgt tatcaagcag aacatagaca gcattttgcg gcagggcaaa gaacaggaac
    57241 ggggaaagga aacagaacga tagcataagc cgacatggta tgctataata actttacctt
    57301 atgttcgctt acaaaaaaga aagaggatag tttaatgaaa tcaatattac ttcatggttt
    57361 agggcaaact tcttcagatt gggataaaac gattgaagtt atggaaaata gaatggatat
    57421 tatttgcccg aatttgtcag agtggcttgt agataaagaa ccctgttata ataccctata
    57481 tcaaatgtta gaaaattatt gtaaccagtt caatgaacct ttgaatttgt gcggtctttc
    57541 tttgggtggt attctcgcta tgcaatatgg aattgaacac actgataggg taaattcgtt
    57601 ggtgctgata ggaacgcaat atactatgcc taaacggtta ttaagatttc aaaatatgtt
    57661 gtttcacata atgcccaagt ctgtattttc acaaattggt ttcccaaaaa aggattttat
    57721 cagtatatgt agttctatga tagaattaga ttttcaacaa agtttgagag atattcaatg
    57781 tccagtgttg gttttgtgcg ggagtaaaga taatgcaaat aaaactgctt ctgtggatat
    57841 ggagaaaatg ataccttgtg caaaattatc tattattcaa aatgcaggac atgaagtaaa
    57901 caaagacaat cctgttgaat taggaaaaca attaaataca tttttcaatc tcaaataaaa
    57961 aagtggacgc agacagccaa caaaagactg tctgcgccct gttttatgta ggtgcgtttt
    58021 gggtatctgt tacgcaatag aacgtcattt ttagggaata aagtataaaa cccttgccac
    58081 gtcacttcca agcactgaaa gccccgtaaa tcaaggcttt ttttgcccct actgcgtaac
    58141 aagggcgcgc cgtttcctca tgcgctccgc tgcctgtctg cgggtaatcc gcttccggca
    58201 gtccgggcag tatttgacgc tgttagaggt ggacgcaaaa gcagctccgc actcggtaca
    58261 acgcttttta tcctcggtct tgaaaagctc tgcatacagc agcttatcaa ggggaagtac
    58321 ggcggtctga aaccatttgc aaagaagcga ataggaaata agctgcgggc agacacattc
    58381 ctccccgtcg tccagtaaaa tacagtgtcc gttatcgcag ttgcagcact ccttttttac
    58441 aaggctgttg actttccggc tttggggcgg cgtaagccgc ttaaccccgg tcatacttca
    58501 tcagcggcga aaatggcaag ctccacatac tcactttctg tcagaggtgc gattttcgca
    58561 agggtgcgct tcgccagttc ccgcatatcc tcgtccatga atggcagcgc agcgttgatg
    58621 ttttccgtaa gcccacgctt gccgccctca ttgtaaatgc tcaacaggtt tgtttcttca
    58681 acggtcagtc taatcatgct catacctcca attcgtgatt ttttgatttt gccgcttttt
    58741 tctgcgcggt cttttctttg tctgctctaa gctgcgcccg gatagagggc ttcttttccg
    58801 ttttcgccgc cttgccgcgt tccttgtcag ccttgacagc ttccgcaagg tcaacaaggg
    58861 aaatggtttc tcctgccttt acctttgctt ccagttctgc ggtggtcggg gtgttgttta
    58921 tctgcccgtc aatcatattg tagttttgct cggtggtctg ctcggcagct ttcagatagt
    58981 tttccggctg ctctgcgaaa atagcaaaag cccttgttcc gttccccata atcaaatatt
    59041 ttccgtcgtc ggactggtgg tgtaagccat atccggcagc ttccatttgc tcgcggctca
    59101 tggcggttac atagaaagtc cccctcggtg ttttgattgt ttcgcccgtt tccaagtcgt
    59161 cgggaatgag ggctttttcc tgttccttga aaaattccgg cacttcctta taaccgaagc
    59221 tgtctacata gtgggcggtg tcctgcccgt tctgatgaag taccaccacg tcggaaacgg
    59281 aaaggctatg tcccttaaaa tctttcgggt ggtcgatatt gaagcgggtg taaatatctt
    59341 caagggaagt ccccggcgta agctctgcgg aatagacaag ggcatagttt tttgggtcta
    59401 cccggtgtcc tgtggcagtc agacggtcgt aaggttcaaa acgcaagtcc cttgtttcgt
    59461 ccccgccttt tatctgataa atggaaaagg tgtctttgtc ctgtggggct ttctgttccg
    59521 gctgctcctg tacgggttgt tcctgtgcag ctaccttttg ctgccggata tgcgcccgct
    59581 gtaactcggt aggcttttct cctgtaaggg gctgaataga tttcacataa tcagccgcat
    59641 aataggcgtt actggtaagc tgccgctgcc atgcccctgc ttttggcgac caacggaagc
    59701 cattactttt taaggcttcc cgcgtggctt cgtccggctt atcctcaaag aagatttgca
    59761 gtcggttcgc ttcggtgttt gcttccacct tgccgccgtc aaattcccaa ccgacaaaac
    59821 cgatttcttt ttgctgtgaa agggatttga tacggtcttt cacgcgccgg atttctgcgc
    59881 tgttgttgga taacgcccaa gtcgcaaagg gcttgtcctc caaatgccaa ctgctcgcca
    59941 tatctgcttt cagtttttcc agttcctccg gcggtaaatg cgggcagccg tcaagggtct
    60001 tatgcttacg gtaataggcg tttacggctt tcatggtttc ctgtgatttt tcaaggcttt
    60061 ccagtttctt ttctaacttc tgtactgcct gtgggtcgtc tgcgctgata ccgcccatgc
    60121 cggtactgcg gattttatca agaagccctt gtatgtcctg ccactcccga taattgctgt
    60181 cgcgggctgc gttctgcttt tctttcttcc ttgtgggaaa gttagagccg cccgcaatca
    60241 ggatagaggg aacacgcgcg tcaatctcat agcctttgtt catgttcgct gccagtttcc
    60301 gggcgtaggt gtcaagcagg ctgtcgattt tctcatggta catcgggtct acccgctgtt
    60361 tctgcctttc tgcgatttgt acggcttcat caacataatg cctgtattca gccgtcgcgc
    60421 tgccttgctt ataatcggaa aagctgtttg cgttctttgc gcggcgggct gcggcttcat
    60481 tgatagtgta ataaggggcg acgggggctg cctgttccgg ctgtgtggtc tgcggtgttt
    60541 cgctacttgc gcctgtatca gcttccggct cggtttgtgc tgccttttcc tgtaccgggg
    60601 ctgtttcttc gggaagctcc ggcgcggcta cttctgcttc gggctgtttg gcggtttcct
    60661 gtgtgggctg tgtctgttcg gtggtctgtt ccttatcctg tgctttttgg atttctgcaa
    60721 aatgtccgtc tatggtatca atcagacttg cggcggtact gcggattgtt tcaagagagc
    60781 cttttaattc tgcaagttcc tttccgctgc tccacccggc gatataccca aaagaatagt
    60841 cggacgtatc aagcccgtag tgttggcaga ctgcataggc gacgctttct gcctgtacct
    60901 cgcgggtgcg gcggtcgggg cgttccggcg cgtctttgtc tatgtcatgg agtgtcgcat
    60961 gggcgatttc atggattgcg gtcttgatgt tctgcaattc gtccatgccc tcgttgatga
    61021 agatacgctt ttcctcataa ttgcagcgtc ctttgatacc gtcattcaat gcttcaaatc
    61081 ctatggcaaa gggggaagtc ttttcaaggg cagcgaaaaa atccttgtat tgttccacgt
    61141 tgcccgtcag ctcataggta agggcaggaa gttcttttcc ctcggtctgt gaaacatcaa
    61201 agacagatac cacacgaaag gcgggtatct tgatttcctt ttcctcggta acaggtttcc
    61261 cgtccttatc gaaaacaggc ttttgggtgt ccgggtcgat tttttccacc tgcttcttaa
    61321 cagtaaacgg ggacggcgca agtatcttta tagccttttc tcccggcttt acatggcggt
    61381 caaattcctt ttcccattgt ttatagcctt tcacaagatt gccgccctgc atggcgataa
    61441 ggattgtatt gttaaggctg taatcatgga attttgacat agtacgtaga tagtcggcgt
    61501 aacggtcgct ttcataaagt tctaaaatcc cctgttccag tcggtcagta atctctttca
    61561 ttttgtcggc gggcttttcg gaagatagga taatcgggag aaccgggggc tgctctgtgg
    61621 cggcggtttg tatggctgcg gaagcgtcaa ggtctgcctg ttccttttcc tgtactggca
    61681 gctgcggtat tacccggtat tcctccggca cgtcccgccc gttataccac tgtgtaaagg
    61741 tgttgcggtt gttgtaaata tagccctgct cggtaaatct gcccccgtcg ttgatagcag
    61801 cgtcccgccc atattcctca tacatgaaat aattttttgc ttcttccggc agctccaact
    61861 cgtccagttc ttcaatcaga taatgcccgt aatcttcctc ggtttttaca gacggataaa
    61921 tccagtaaca gtcaaggttt tgtgcaaggt tgataatgtc ctgcaagtcc cttgtatggt
    61981 cgccgtatgt gatagccgca gcaaattttt cccggtcgtc gtcaaactgc attttcaaaa
    62041 gctctcccaa atagttcagc tcgtcaaggc tttcaagctc ggtcaatttt tctgccagtc
    62101 ccggaataga ggactgatat tctgtgatgt gcagttcccc gtagtgtttg ccgtctatcc
    62161 cgatacggtc aaagacttct ttcaaatgct cggcagtcgt ggggaatgat acccattcgc
    62221 ccataggtct gccctcggtg tacttcccaa gattagaaag atacccgctt aaaatcgttt
    62281 cttccatgcg ctcactcctt tctttctcaa tcatgcggtg cataagttcg tagtcctcca
    62341 tgctcatgct cccgtcaaaa atataggggt cgggttcttc gccgtcccgg taggcttttt
    62401 cagagaaagg cagccccgca gcgtgggcgg cggctcttgc ttcctcgata agctccggcg
    62461 gcagcctgtc gtcgtgggct tcgataaatt ccctgatatt gctatatccg ttttcatagt
    62521 ggcggcgcac ccctgcggtc agctcatcaa ggttcctatc ctcgccaaga tagccggaat
    62581 aataaccgtt taccacaatg gcggcggggt cagcctgttt gatttcttcc atgcggcttc
    62641 tgtcctccgg gtagaggtat tcgcccaatt caaggtggaa atcttcggcg ttccaagagc
    62701 gtcccgtttt ccaaaacgcc acccaagcga taccgtcccg caattcttct tgatactcct
    62761 ttacggtgtc ccgtaaactt gccatgtgta acctcctttc caaagggcag cgcggacgct
    62821 gcatttccca gtttcatcat tcagtacccc gcgcccgcgt ttttgccaaa gagtttagat
    62881 attttttaat atttcagagg gtttgggaca cttcccaaca agcaaaaatc cccgccgttc
    62941 tcggtagaga aagcagggat tttacggaaa agggtactct tttcctatgc ttgctattca
    63001 gttatttttt cttcggaagc tccacccggt agttgacata tttcccgccc gtatcagcca
    63061 atacgatagt tccgtcgtag gttttgcccg tctttgggga atagagcttt ttcacattta
    63121 ccttgccgga ttgtaagagt gcagcggcaa tcttcggggt aaaggttact tttcgttctt
    63181 caaagaaacg gtcatttttc cacatggtaa agatacagtc cttgttgctg caatagtagt
    63241 ttttctttcc ctcatagaca ggagaaccgc agcgggggca gtttcccaac acctcccgtt
    63301 ccggcttgaa catctgcgct ttatcatcag aaagaaacgg ataggtcttt accagttccc
    63361 gcgccatatt ttcgataccc tccataaatg cagcggggtc tgccttgcct tttgcaatct
    63421 gcgtcagatt gttttcccat tctgcggtaa gctgcgggct tgtcaatgtg tccggcagga
    63481 cgcacacaag gttgataccg tcttttgtgg gaagtagctg cttgcctttt cgctccacaa
    63541 agccgccctt taccagtttt tcaatgacgg cggcgcgggt ggcgggagtt ccaagccctt
    63601 tgcgttctgc gtccgggtcg gtgtcctcac ttccggcacg ctccatagca gagagcagcg
    63661 acgcttcgtt gtgaggtttt ggcggtgttg tgtcatgctc cgttaccgtt gccgccggat
    63721 tttcaaaggt ctgtccatcg gcaaacggcg gcagggtatt ttctgtgtcg ctgtttgcgt
    63781 cgtccgtttc gggcttgttc tttaaggctg ccctgtatct tcggtcaatc tctttccacc
    63841 cctcacaaag aatggtcttg ccccttgcgg taaaggactg tccggcacat tcaaaaactg
    63901 ctgtaaccgc ttcaaaggta tgcggtgcag cggtcgccat gataagacgc gccccggcaa
    63961 gggtaaggat attcctttca ctttccggca gcgcggcaag gtcagtcttt gcaagctcca
    64021 tagtcgggat aatggcatgg tggtctgata cctttttact gttcagaacc tttccaagct
    64081 ccggcgtgaa gtcttttccc gccataaaag aaaatttccc acaaagcagc ttgataatgc
    64141 ccgccgctgt gtcgcccatg tcgtcagtca gatagctgct gtctgttctc ggataagtaa
    64201 gcaaacgctt ttcatacagg gcttgtgcaa ggtcaagggt ctgctttgcg gtataaccga
    64261 acaggcggtt tgcttccctc tgtaaagaag taaggtcaaa gggcttcggc ggggctgttg
    64321 tctttttctc tttcacaagg gaagtacaga ctgcccgcga cgcttcgcaa gtcgttttta
    64381 atgcgtcggc ttctgcctta tctgaaattc tttcacttgc agcttccgcg ccggataagg
    64441 taaggcgcac atggtagtat ttttctttct tgaaagtggt aatcgctgcg tcccggtcaa
    64501 caagcatttt taaggtcggg gtctgtacgc gccccacgtt caaggtatga ttgtaaagga
    64561 cagagaaaag acgggtggcg ttgataccga taagccagtc agcctttgcc ctgcataatg
    64621 cggaagcaaa gagcgcgtcg tattcctcgc cgttcttcag acgggaaaag ccctctttga
    64681 ttgcactgtc ctccatagag gaaatccaaa ggcggcgcat aggtttcttg cagcccgcca
    64741 tttcatagac aaagcggaaa atgagttctc cctcacgccc tgcgtcgcag gcattgacga
    64801 cttcggaaac gtctgcccgg tgcataagct cttttagggt tttgaattgc ttttccttgt
    64861 cagaagcaac ggtgtacttc cattcctgcg gcaaaatcgg taagctgtca tagctccact
    64921 ttttatactg ttccccatag gcggcagctt ccgcaagccc taccaaatgt cccacgcacc
    64981 aagaaacgat atagccgctt cctgtgagaa atccgtcttt cttttccttt gctccaagta
    65041 cggcagcgat tgtctgtgcc acactgggct tttctgcgat aactaagata tgtgaagtca
    65101 ataaaaaatc ctcctttcag cgttccggct cggtttcttt tttcttctgt acctgtttca
    65161 gacagtaccc cacacaaggg gactgcccct tgtaagggca ggaagcgcaa gccgggggat
    65221 aaggaacagg cggcgcatta tcacgccgcc cgtccggttt ctgtatcatc atcttttcaa
    65281 agggattgtc ggtaaacagg gtcatagggt ttcttcctcc gtttcttcat cttcggaagt
    65341 ttccccggta tctgcttccg gctcgtcctc gtcgtagtcc tcaaattcaa aatcttcaag
    65401 gtcggtatcg ccctttacat tctgcttcgg ctttagaaac ttaaagtagt agaacgctgc
    65461 gccgccacca agcagggcaa agaggactaa ggcaagcacc acaccgggat tgccgccttt
    65521 ttctttcggt tcttccggct gctcggttgg tgttggggtg ggttctttgc ctgtgcagcc
    65581 tgtcatactg gtaacacata ccgggcagga cgtgtttacc tttccggctt cgcatttttc
    65641 cgtacagtta cagatttccg gttttttggc agcttcgccg ttttcggtca gtgccataag
    65701 gtctgcttcg tccacttgat taaggaaatg tacggtctgt tcgccctcgg cggcgcggtc
    65761 aatgaggata tagaagtagt tgccgctttt ggtggtaaca gtgataagct gcttattgcc
    65821 gccgtaatcg tcaaccagtg ttgcgtttcc ctttggggta aggggtggcg tttcttcttt
    65881 ttcctccaca actacgttgc tgtcgttggt cgcattatct gcgggcggct ctgcggctgt
    65941 tccctgcgca taggcaggga cagaaaaacc gcccataagg ataagggcgg cacaaagggc
    66001 agtcatggtc tttttcagtt tattcttcat ctgtattttc ctcctgttct tcgtagtctg
    66061 ccgtttcggt agcgggtgcg gcgttcatgc cgggaatacc gccgccggac agcatagcgt
    66121 taagttcctg cggggtcaga cgcatagctc gtacaagctg cacgatttca aggttttccg
    66181 cttcggtttt ctgcgcttcc agtcctttta acttgttctg atactctgtg attttctcgc
    66241 gggtctttgc gatttcctta ttgatacggt cgattttatt gtttgccata ggtcatttct
    66301 cctttctttt tggggctgct ttaccagttc atcagcccgt agcctttgat acattgatag
    66361 ttaaggtcat agctctttat cttgcaggcg tcgccggaat tgccctcaac ggtatagacg
    66421 cggctttcgt ccctgccaag cacaagcccc acatggtcgg caacgccgtc taaatcccaa
    66481 tcgaaaaaga tagcgtcgcc cggtgcgata ttctcatagc cccgcgcgcc ccattgtccc
    66541 cttgactgaa accaagggac accctgccat tcgcagcccg caaagcgcgg ttcgcttttt
    66601 ccggcttgat tgtagcacca tgatacaaaa caggcgcacc attccacgcg gctgttaaag
    66661 ccgtaccaag accagtaagg gtagccgccc acatttccga cttgctgctt tgcaagctcc
    66721 ataagctctt gatttccggg gcgcgtaccg tttacaaact ccacgccgct taaatcctcg
    66781 gacgggcttc cgtcgggaga gccgccgcca aaaatcaatg gtttgttgcc ctgtgtctgt
    66841 aagtacactt tgaacatttc aaactgctgt gggttcagca gttcttccgc aagggaagca
    66901 atcggcttgt ttgtcagctt cacgttcaag atacggtatt cgtattcttc ctcgttgccc
    66961 tcctcgtcgg tggtggtgcg tatctcgatt tcttccgtca gcgtcaaggt gtactgtaag
    67021 ccaaacagcc gctttagctc tgcctgtgcg ctctgcggga tgtaactctg caaaagggcg
    67081 gtcagataag acgcaagctc atgtgggtta tgcccgatag tatcaaggtc atagcggtat
    67141 tcatcatagc cactgtgggt actttcgata gcgtcaatct cactttgcag ctcgttttcc
    67201 attgcagcat agctctgttc cgttgccaca aggtcgctgt cctccgacgt ataggacgtg
    67261 ccaagcacgc cgtttgtcaa gcccgaaaac atagccccgc aagaggacag ccctgcggaa
    67321 atcataataa agagaagcag tgcggcaact gcaatcaaaa tgcccgccgg gtgtcttgcc
    67381 gcaaatgcgg cggctttcct cgtttcctcg gcggttttct tcgccgtctt tcttgtggct
    67441 tccgctgcgg cttttgcccc tcctttcttt gccgactttg catactggcg ttttatctgc
    67501 tgtttctgca tgaaacggga aagcgggtta gaagtaagct gcgggttgtc gtgcagggtc
    67561 ttgtgatact ggaagttgac gtttgcctta aatgctgcct tttctgcctt tgccgcttcc
    67621 ctgtaaggtt tcagcttatg gctgcggtag ccctgtttta acttcctcgc cccgtatttt
    67681 gcgccgcgct cggcaagttc ctccgatttg tgcgcgccct ccaagccgga attatctttt
    67741 tcgacgctgt ggattttatt gtgaacaaaa atacccgctt cctgcgcagg gcgggataac
    67801 gggttcctgt gggctttgcc gccgggtatg ggcttttcct gttcctcaaa atacagacgg
    67861 gtctttgcct tacctgtggc ttcctcaaag gtgcgctctt ttacaagttt cttctgcttc
    67921 gggatagctg cctttgcttc gtccagtttg tcggctgctt tttctgattt tttgatatac
    67981 ggttccagtt ccggcgttgt ccgttcctcg tcggtaaact gtaagcggga agtggaagta
    68041 cgcaaagcag tagcttcctg ctctttcttg actgcctttt tgtttgcctt tcgggtatgt
    68101 gctgcgtcaa ggtgttccat gacgcgctcg gctgcggcat tatccggctg tgaaaagtcg
    68161 ctgtctgctt cccggctgct gatacgctcg gcggtctgct gtgtttcgtt gatttccacc
    68221 gcaccgtcgc gggtcatttt ctgtgtgatt ttatcgcgtg gttttaactg tttcataggc
    68281 tgctcacttc ctccggcttc gtcgtcataa tgcggtaaag ctcggtatct ttcgggaaat
    68341 ggtctacaaa gggcagcact acatttccat aaaagataag tccctcgcct gcttcggtgt
    68401 gagttacata cttcatctgc tgcgggctga tattgagctg ctttgcaagg atagcccggt
    68461 cgccctgtgc ctgattgagc atgaggacaa aatcagagtt ttcaaagata ttttccacct
    68521 cgcgggaagc aagcaagtcc ttaatgttct gcgtgatagc tgtgggtatc tgttgtgtga
    68581 ttaacggaca gttttttatc tgtccctccg gggctttcgc ccattttcag cggtatgtct
    68641 gttcataccc ggcatagcat atctttttat cctgcggttt atgccgcaga ccgaaacgat
    68701 agcggagcct cgtggggctg ttatattccc ggctgttgca aaccgggggt caagccctat
    68761 gcgttgcgtg tgtatcaagt attaccttga cacttccact cggattagga ttgcaaaacc
    68821 tttcccgttt tcttctccgc tgatattctt aaccgttctt ttcggctccg gttaagacgg
    68881 catacacaat ccccatttgc aagattgtag ggactgtgcc tgtttacctc cccattttct
    68941 aaaacgcttc caaatctcca cactgtaagc ggcggtctgt tcctctttta agagcagatg
    69001 aaattcgtcc atatagtagc gggtagattt cttttctgcc cggttaatgg taacgcggtt
    69061 ccatatctgg tcttgcacaa tgagcatacc taacttttta agctgttttc caagctgctt
    69121 aatgtcaaag cagacaagac ggttattcag ttccacattt gttctgtggt tgaacacgtt
    69181 aaggctgccg gaaacataca gctccaatgc cgccgcaatg cgggcagctt ccggctccgg
    69241 ctgttttaac agctcgtcgt aaaggtcgcc caagataggc atttttgccg ggtcggggtc
    69301 agcaaggtag tcccggtaca cattccttac agcgcggtca atgacggtct tatcaacggg
    69361 ctgcaagccc tccttgccgc caatgacaag ctcgcagagg gaaaggataa aatcactttt
    69421 cagtgcaagg gggctgtcgt cctcgctgta attaaggttc atatccatag ggttcacata
    69481 ctggggcgta ccgtccatgc ctttcccggt cggggataag cgtatcactt gcccgccaag
    69541 ccgcttgaca agggaaaaat actcggcttc cgggtcgcag ataaaaatat cgtcgtcggt
    69601 aatgagaaag gcgtttgtga tttcccgctt tgccgcaaag gattttccgc tgcccggtgt
    69661 tcccaagata agcccattcg ggtttttaag ctgcttgcgg tcgcagagta tcatgttgtt
    69721 gcttaaagcg ttcagcccat aatacagggc agccccggtc tgaaatagct cctgcgtgat
    69781 gaacgggata aaaatagcgg tgctgctcgt cgtcaaacct ctctgaatag ggataaggtt
    69841 ctcccccaaa gggacagagg acatcagccc cgcttcctgc tgatagtcaa gacgggtcaa
    69901 ggcgcagttg tatttctgtg caatacccgc cgccgcgaaa atgtcatttt ccagtttccg
    69961 tttcgtgtct gccatgttta ccacaaggaa cgtaaggaga aacattcgct cgttgcggct
    70021 ctgtaaatcc tgcaataaat tctttgcttc atttccaaaa gtggcaaggt cggacgggat
    70081 tatatccatg tcataaccgc ttctaactgc ttttttctgt tcctcaatct tcatcttgtc
    70141 aaggtcagtg attttgcgct taatggtctt gattgcttcc gactggtcga tactgcggat
    70201 atgtaagttg acgattacgc ctgtttccaa atctaacata tccgctaaga tacggtcgtt
    70261 tagctccggg gcaaggattt caaggaacga taccgccccc gtctttttcc ccatagcaaa
    70321 gtagcgtccc tccccgaact taaaagagga cggggcgata aagtcctttg tggtaagccc
    70381 tgtcggggtc agccagtcat aagagaatga gaacggctcg cctgccgggt ggaatacccc
    70441 atgcagcacc tttaggcgtt catagccgga taaggggtgc gccgatacgc caagcacctt
    70501 gaagttattg agtacgtcgg tttcgatacg ggaaagccgc gcccttgccg ccgccggatt
    70561 gtccgcttca atggaaaatg tgatgtactt atgctttaca agcccgttgt tccctttggc
    70621 aagctgcgtt ttcagcatat cggaatactc agtgcggata gagttaaaat cgtcattttg
    70681 gggcgggata tggattgccc gcgctgcctg ttcctgctgt gagccttgat tgatgaaaga
    70741 aagctgcacg ttcacgcttg cgtcaaagta gttgagaaaa tcgcaccagt tttcaaaaat
    70801 ggcggtctta tcatctgcct gtgcaagctg atagttgatg tcctcaaagg caatgctctt
    70861 tgaatacttc cgctgtgcca ctttgcagat accgtccggg tacatggcaa ggtagggaat
    70921 ggtctgctgt gcggtgtggg ctttgccgtc cccctttgcc tgccggataa ccgccgctat
    70981 ctgtttcttt tctgcgcggg tcagcttacg cctgtttcct gcggcttccc gcgtcttttt
    71041 tgtctgtcct tttgacaatc gctgatacct ccttttctaa tgcccgctgt ttctccaaga
    71101 gtgcatatag gttttccgtc tgatacggtc gcactttggg acgggtaaat tttgtctgaa
    71161 tgatgttctt tacaaccact tcaaggggct gtccatgctt ttcatacatg gcaaccaaga
    71221 agcagggaag cataacaaca atcattgcca ttgccgcaag gctcgtccct gcgctgtctt
    71281 tgagcaaaaa gaaaagcggc agtcccataa gaagtgccac tgaaaaacaa aggatttgcc
    71341 gctttgtcag attgaaagcg acttttgttt tgactttgga taagtctttg ggtacaggta
    71401 catacgccaa gaaaaaacct cctttctgcc cttagtgggc gttgaatata gattttgcca
    71461 gtgcgccgga tttgaacagg gaaaaacaga ggataacggt ataggctgca agggaaaaaa
    71521 tcgcgctgtg caggttatcc gctaagacca tttcgcctac caaaactgcg taaatgccga
    71581 cgcatatcat aatgaggaag ccttgaaagc ccagtgcaaa cagtcccttt aggtagttgt
    71641 tcccaatctg tccccattcc cggtttgtca tggtggcaaa ggggataggg gaaacggaac
    71701 agtaggcgta tatctcaatc attctgccgt agaggataac ggttatcagt acggacatga
    71761 ttttcataca aaggcttaca aggctcgttt ccatgacaag taacagtagt tcggggattt
    71821 ccatatcttt tagtccctcc tgcatggcgg caagggcttc ggttacgtcg atattcgtat
    71881 cgccgccgat tacccccgcc gcaccggaca caatatgctg cgccatatca aagactgcca
    71941 ttgttatatc aaaggtatgc gttaccaaga aaacggcaac aaaggctttg aaaaaccact
    72001 taaagaacat gaatgtttct atgtcgtgca tattgttttt ttccgttacc atgctgataa
    72061 gctcataaca gaggacatag gtaatgacaa gccccgcaat aggtacaatc acattttcgg
    72121 acagattttg tatcatgttg aaaatgtttg tgttccaccc ctgcggggtc ttgcctacct
    72181 ccgctgcaat ggtgcctact ttttcgttta cgtccccgaa catagtggaa aggttaccgt
    72241 taatggctcc gataaggatt tcttttatcc attcgttaat cgcttccagt atgctctcca
    72301 taggctaccc tccgtaatct tatccgaaca gaccggaaag aagcggtaca aggatagtgc
    72361 cgataagggc aacgccgccg cccgccatga gctgcttaat gccttgactt ttcgcacccg
    72421 gattgtcgtt tccatatccc tccaagagat tgataacgcc ccaaatgcct aagcctgcgc
    72481 ccagtgcgat aacaagggtc tgtaatacgt cgatagcaga gttaaaaaat tccataaagc
    72541 tcctttctgc cgcaaatgcg gcttgccccg taaaagggca taaaaaacgg cggtcagttt
    72601 tcaaactgcc gcggtcataa gtccccgcgc tgcgtaaagt tctgccgcgc ggcggtattc
    72661 agttgtcagt gtgggcgata ggaatttttg taggggcgcg tatcatgcaa gttgtctgtt
    72721 tctttgttac tgcgtcaatt cctcctttct gttcgctgct gctcatacag ggcttcacac
    72781 cgttttacaa atcgccggat agcagcctgc cgctgctgtt cccgtcgttc agcaatcaaa
    72841 acaagcccgt ttatggcttt tgtgattttc ttgtcctttt tctttctgat acgtttattc
    72901 atggtcggtg tcctcctgca aatcgctttc gctgatttcg tagtagtcaa atacttcgtc
    72961 cggcttcaca agggcgggtc tgcgccttaa atgcttttcc atatcaaaag tatttttctt
    73021 gtcatagtcg gaaagatact tatagttggg gtgctttgta atgtcgtact tatcagagaa
    73081 gaacgggcga acccctcgta gctgcaaaat acatttcccg ccgtccatga ccgctatttc
    73141 atcttccgtc ataagctgct tgcctaactt ctgatagttc agcccatgtg atacctcccg
    73201 cccccggttc tcggaagtat tgaagctgtc aatggtttct ttccccaaga tttccgacat
    73261 ttctttgagg gtggttttct ccttgccgcc caagaacagg gtggtgtcgc agttgccgac
    73321 tatggtatcg gcgttgtcct tgtaaatggc ttttagctgc gactggctct gcaaaatgat
    73381 tgaagcggag atttcccggc ttcgtatggt ggctatgagc ttttcaaact tcggtatctg
    73441 cccgatattt gcaaactcgt ctaacagaca gcgcacatgg acgggcagcc tgccgccgta
    73501 ttcatcatct gccttgtcgc aaagcagatt aaaaagttgt gtgtaaagga ttgataccac
    73561 aaagttaaaa gtgtcgtcgg tgtcgctgat aatgacaaac agggcggtct tgcggtcgcc
    73621 tatggtgtca agctccattt cgtcggtttc cataaggtcg cgcagctctt taatgtcaaa
    73681 aggggcaagc cgcgcaccgc aggaaatgag gatagagctt cttgtctttc ccgcagataa
    73741 caggaatttc ttatactgcc gaacagcaaa gtgttccggg tctttttcct ccaaccgttc
    73801 aaacatgagg tcaacggggg actgaaattc cgggtcgtcc tcgcgggctt cactggcatt
    73861 tatcatttca agcagcgtcg tgaagttctt ttcttcctcc ggggcttcgt accaaatgta
    73921 gccgataagg gcgcagtaaa agagccgttc cgattttacc caaaaatcct ccccggcttt
    73981 ttccccgtcc cctttggtgt tggcaatcaa agtgtttacc agtttcaaaa tgtctttctc
    74041 gctgcggaga taggcaaagg gattgtagcg catggatttc ttgaaattga ttgtgttcag
    74101 cacctttatc cgatacccgc cgcgctgtaa gagcttcccg cactcgacta aaaccgtacc
    74161 ttttgggtcg gttacaacat agcttgagtg catttgcatt aaattcggtt tcacaaaaaa
    74221 tcgcgtcttg ccgctgccgc ttcccccgat aacaaggata tttttattcc ttgcatactt
    74281 cggctgcttt gggcggctgt tcatggtaag ccgttccgtc tgtgtcagca gcacgttatt
    74341 ttgaaatacc gggtcggtgt agggttttat atcttcggcg ttgccccaac gggcagagcc
    74401 gtattcgata cctttgcggt atttcttcgc gttcttgcct tttacataga caatcagccg
    74461 gataaggaca gcccccgcaa taccgataag caaatccata ggggcaaggc tcggaaacgg
    74521 gtttgcaaag gcagcggaaa agccgtctgc aagggaaagc accttgccgg agaagtcagc
    74581 ccccggcgca aggcgtaccg cctgcccgac tttatcaaag agatacacaa agagaagata
    74641 gggaatgttg ggaagcagca gctttttcca gttgatagcg ttcatcttgc aatccccctg
    74701 tctttgtttt ttactttgtc ccgctgtgcg ttcagagccg ccgccttatc tttcagagtg
    74761 gaaagcagct tgcggataga gggcttttgt tcctgcgtca gcttctttgc ggaaaactcc
    74821 ttaaaggctg cggtcagtgc gtccgcgtcc cgtcccttga aaaacacaag ataacggggc
    74881 ggggtttcgg tgctgtcttt ctttagggca aagtcaatcc cgtatttttt cgccgtactc
    74941 tcaaaggctt tgatattgtc ctttgtgatt tcaatgttgg aaacgcccgc gttctgcttc
    75001 ataagctgct ttagggtctg ctttccatgc gggattttct gtctgtcatg ctgctttttc
    75061 atctgtgcaa gcagggcttt catagccttt tggagctgct gcgccgtcag cttcccggtc
    75121 ttgatgtaaa gggctatggt cttttcgttg gtttcctcct gcaatgggtg tcctcctttc
    75181 gtgttctatg gtggtatgcc cgccgccgga aaatggcgcg ggcatgaaaa aagagcgtcc
    75241 gttttcacgc cgccctgtta aatccgtacc cgcagcccgg aaaggtctgc tgcccgaata
    75301 cccacaagat accagttgtc cccgtactcc atacgggtcg gcttccattt gccccgcaca
    75361 aagacctcca tgcagtcgcc gcaatgcaag cccccgtaat agtcgtttac gtcaaagcga
    75421 atgtcgtagc ggtcagcggt ttcatcaaaa atcaaagctc ctgttttctg tgtcatcata
    75481 aaatcctcct ttgggttggt ttacaggctt tcgccctcgt tgcaggtgta atcgggtatg
    75541 cccttgcttc ttgccgacgg gatagtgcgc ccgtcgtaca tatcgtgaga tacaagggac
    75601 gtgtaatagc tgccaatcgt agacggggca ttgaaaaggg cagctttcat gtactgcttg
    75661 atgttgcgga ttttggtggt gttttcctgc atacagtcca gtacaaagcg gatatgttcg
    75721 ctgttcagtt tcataaactt tgcttttaca agctctgccg ggtggtcgtc cccggcgata
    75781 cggattgttc ttcgtttggt gcagacggtt tcaagaataa gggcaaggat ttcctctatc
    75841 ctgtcccggt caaggtatct gtcctgtatc agatagtcgt actcgatatt gtccttgatg
    75901 atttcctcat acacgctata tgcgtctgtc gcttcctttc gcttccgttc cggcggctgt
    75961 gccgcgtcct ctaagggaga gggatttagg gaatggaaag gaatggaatg gatacttgat
    76021 atatctgtat ttaatttttc ttttgttggt aagttagttt tttgtatctc tttatttaat
    76081 tgcgttggat tttccaacat aggcttttcc aatgttggat tatccaatgt tggtttttcc
    76141 aatgtaggta aatccaactt aggcggctgc tcatagataa catacgttgt accgcgtaag
    76201 cgtcctttct catcacgttc ccttgaacgc tgtatatatc cggcattttc cagttcccgt
    76261 actgcttccc ggatagcgtc aatcttttcc cggttgataa gggacagtcc tgcaagggtg
    76321 tagtcccaat cttcgggaag tgagagcatt tgcgacaaca agccctttgc ctttagggaa
    76381 agctccttgt tgcgtaaatg gtggttgctc ataaccgtat aacctttgtt cttttccaca
    76441 cggaaaactg ccatagcttc atcactcctt tggttggatt tgttacgcag tagaaatgcg
    76501 gttttgaggg aaaaggtatc aacccttgtc tttcttattt ccgcgctcta agagccgcat
    76561 aaatcaaggc tttgtttgcc cttactgcgt aacatgaggg cataaaaaaa gcagcgttcc
    76621 tgttctcctg tgtaggagtg agaaacgctg ccttataggt ggtgcttatt ttattttttc
    76681 ttttcgacgt gccgcgtaag gtggtgcagc ggtctatccg gcagcttcgc aggtgctttt
    76741 gcatatcaag gctcaatccg ttaaaagtag taaatgagta gtaaaatcaa tctgcaaacc
    76801 tgcgaatatg cctgtaaatc aagcattttt caagataatc aactaatttt tcttaaattt
    76861 atatatcatt ttgacttggt taaacaattt catacttatc gatataaaaa taggttataa
    76921 tataaatgtt cattatctta taacctattt tatataattt attcctaaaa catgtgaatg
    76981 ttttttatga tagtttgaag aaattataaa atcactttat aattgaatat tatatataaa
    77041 ttaatttaaa tatagaaagg aaaatatatg tacaacaata tcaaaaatat tttaaaagaa
    77101 aatcatataa cgtttgaaga atatgaccat gaacctattt tatcatttga aaaagctaaa
    77161 gaaataagag aaaaattaca atatactgga gtagaaagta aaagtttatt tataaaagat
    77221 aggtctggaa attactatat atttgtaacc acagaaggaa aaaaattaga tccaaaaact
    77281 ataaaaaatg ttttaggtgg aaaagtatct atatgttcaa aagaagaatt aacacaaaaa
    77341 acacaatgtt tgcctggatg tgtatctcct tttggatatg agaaaaatat ctctcttgta
    77401 atagatcaag atatatttaa ccatgataaa ataatctttt caccagggat accagaaaag
    77461 acatttatta ttacagctag tggatttaaa aaagtaataa acaatataaa caatacaact
    77521 tataaatata tatatattaa tgaattttaa tcataattgt aaattagaga aaataacatt
    77581 agaaatcatg cttaactata gtgtaaataa attatattca acacaaaaga ttgatactat
    77641 tattacaata gttactgtta agctcaaatt caatgtatta tgagttatta tttttaaatt
    77701 ttaattacaa aagcagggta gttttaacta ctctgcttta tatttaacat aaaatatata
    77761 aaatcaacaa aatgatttta gtctatctat tagatattct tcgctctcaa tgcttagaac
    77821 ttttagatat atacaataag tgctttcatt tgtaaaaatt tctatagtat tatcttgttt
    77881 atttacacct acaatatctt taaaattgat atgattttta gtatttttac atttgaaaat
    77941 atatctg
//
LOCUS       DAELJO010000002        69829 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELJO010000002 DAELJO010000000
VERSION     DAELJO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.002"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(438..2198)
                     /locus_tag="KPB88_000086"
     CDS             complement(438..2198)
                     /locus_tag="KPB88_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0052402.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(2261..2404)
                     /locus_tag="KPB88_000087"
     CDS             complement(2261..2404)
                     /locus_tag="KPB88_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0052403.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(2723..2962)
                     /locus_tag="KPB88_000088"
     CDS             complement(2723..2962)
                     /locus_tag="KPB88_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052404.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(2967..3383)
                     /locus_tag="KPB88_000089"
     CDS             complement(2967..3383)
                     /locus_tag="KPB88_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0052405.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(3380..3679)
                     /locus_tag="KPB88_000090"
     CDS             complement(3380..3679)
                     /locus_tag="KPB88_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0052406.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(4730..4897)
                     /locus_tag="KPB88_000091"
     CDS             complement(4730..4897)
                     /locus_tag="KPB88_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052407.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(4910..7675)
                     /gene="mgtA"
                     /locus_tag="KPB88_000092"
     CDS             complement(4910..7675)
                     /gene="mgtA"
                     /locus_tag="KPB88_000092"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG0052408.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(7841..8152)
                     /locus_tag="KPB88_000093"
     CDS             complement(7841..8152)
                     /locus_tag="KPB88_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0052409.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(8399..9076)
                     /locus_tag="KPB88_000094"
     CDS             complement(8399..9076)
                     /locus_tag="KPB88_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0052410.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(9214..9717)
                     /locus_tag="KPB88_000095"
     CDS             complement(9214..9717)
                     /locus_tag="KPB88_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0052411.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(9733..12396)
                     /gene="mgtA"
                     /locus_tag="KPB88_000096"
     CDS             complement(9733..12396)
                     /gene="mgtA"
                     /locus_tag="KPB88_000096"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG0052412.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(12489..12698)
                     /locus_tag="KPB88_000097"
     CDS             complement(12489..12698)
                     /locus_tag="KPB88_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052413.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            12648..12983
                     /locus_tag="KPB88_000098"
     CDS             12648..12983
                     /locus_tag="KPB88_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052414.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(13182..14084)
                     /locus_tag="KPB88_000099"
     CDS             complement(13182..14084)
                     /locus_tag="KPB88_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0052415.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(14101..14547)
                     /locus_tag="KPB88_000100"
     CDS             complement(14101..14547)
                     /locus_tag="KPB88_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0052416.1"
                     /translation="MTSKNYLSLLELVNQYLTVPQVSGELAQKVMNEALKYQGWKYVF
                     GGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQAKAGDLVFFHSTYN
                     AGSYVTHVGIYVGNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVKQ"
     gene            complement(14642..16507)
                     /gene="ltrA"
                     /locus_tag="KPB88_000101"
     CDS             complement(14642..16507)
                     /gene="ltrA"
                     /locus_tag="KPB88_000101"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG0052417.1"
                     /translation="MNRVKTSKTLRHSEYYDMTETFDRLYEASQNQQIFVDIMSVIMS
                     DENIMLAYRNIKKNKGSNTKGTDKQTIQNIELMSEKEFVQKIKDMFCCYTPKPVKRVN
                     IPKPNGKLRPLGIPTIWDRIIQQCVLQVLEPICEAKFHNNSYGFRPNRSAHHAIAQCY
                     RLMNQSKLQYVVDIDIKSFFDNVNHAKLIRQMWTMGIRDKHLLGIIRAMLHAPIILTD
                     GKIEHPKKGTPQGGTLSPLLSNIVLNELDWWIASQWEEIPTKSKSARVLDRRDKGKGI
                     DKGNKYKELRKSTLKEIYIVRYADDFKIFCRKRKDADKIFHAVIQWLKQRLSLDISEE
                     KSKIVNLRKQKSEFLGFEMKLVRKADKYVVQSHMSKKAMNKAENNLKEQIKRIQHPAN
                     DREQAKEISIYNSMVVGIHNYYNKATLIAFDCSKVNYIISNWIDNRLNVTKQGAIQNK
                     YLSKKYGKSQQIRWLNNCPIIPIGYVKHSKAISIKNEICKFTPKGRELIHKSLDIDVG
                     VLVQLMKNPILDMSVEYNDNRISKYTAQLGRCAVLGVSLSFQEIHCHHIKPISDGGTD
                     KFQNLIIVHESIHKLIYATSQEIISKITSKFKLNKKQISKINALRIKVNNGVITV"
     gene            complement(17221..17808)
                     /locus_tag="KPB88_000102"
                     /pseudo
     CDS             complement(17221..17808)
                     /locus_tag="KPB88_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            complement(17805..20009)
                     /locus_tag="KPB88_000103"
     CDS             complement(17805..20009)
                     /locus_tag="KPB88_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0052418.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(20009..22459)
                     /locus_tag="KPB88_000104"
     CDS             complement(20009..22459)
                     /locus_tag="KPB88_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0052419.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(22407..22835)
                     /locus_tag="KPB88_000105"
     CDS             complement(22407..22835)
                     /locus_tag="KPB88_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0052420.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSIIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIYRQQPCLEQGQ"
     gene            complement(22953..23456)
                     /locus_tag="KPB88_000106"
     CDS             complement(22953..23456)
                     /locus_tag="KPB88_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0052421.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(23474..23977)
                     /locus_tag="KPB88_000107"
     CDS             complement(23474..23977)
                     /locus_tag="KPB88_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0052422.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(23896..24192)
                     /locus_tag="KPB88_000108"
     CDS             complement(23896..24192)
                     /locus_tag="KPB88_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0052423.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(24291..25064)
                     /locus_tag="KPB88_000109"
     CDS             complement(24291..25064)
                     /locus_tag="KPB88_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052424.1"
                     /translation="MKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(25124..25345)
                     /locus_tag="KPB88_000110"
     CDS             complement(25124..25345)
                     /locus_tag="KPB88_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052425.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(25346..25480)
                     /locus_tag="KPB88_000111"
     CDS             complement(25346..25480)
                     /locus_tag="KPB88_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0052426.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            complement(25493..26689)
                     /locus_tag="KPB88_000112"
     CDS             complement(25493..26689)
                     /locus_tag="KPB88_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0052427.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(26874..28268)
                     /locus_tag="KPB88_000113"
     CDS             complement(26874..28268)
                     /locus_tag="KPB88_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0052428.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(28362..28799)
                     /locus_tag="KPB88_000114"
     CDS             complement(28362..28799)
                     /locus_tag="KPB88_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052429.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(28901..29284)
                     /locus_tag="KPB88_000115"
     CDS             complement(28901..29284)
                     /locus_tag="KPB88_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0052430.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(29300..29626)
                     /locus_tag="KPB88_000116"
     CDS             complement(29300..29626)
                     /locus_tag="KPB88_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0052431.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(29752..32796)
                     /locus_tag="KPB88_000117"
     CDS             complement(29752..32796)
                     /locus_tag="KPB88_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0052432.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(33039..34403)
                     /gene="rlmD"
                     /locus_tag="KPB88_000118"
     CDS             complement(33039..34403)
                     /gene="rlmD"
                     /locus_tag="KPB88_000118"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0052433.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(34578..36338)
                     /gene="pyk"
                     /locus_tag="KPB88_000119"
     CDS             complement(34578..36338)
                     /gene="pyk"
                     /locus_tag="KPB88_000119"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG0052434.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(36390..37349)
                     /gene="pfkA"
                     /locus_tag="KPB88_000120"
     CDS             complement(36390..37349)
                     /gene="pfkA"
                     /locus_tag="KPB88_000120"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0052435.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(37558..41127)
                     /locus_tag="KPB88_000121"
     CDS             complement(37558..41127)
                     /locus_tag="KPB88_000121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG0052436.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(41284..42231)
                     /gene="whiA"
                     /locus_tag="KPB88_000122"
     CDS             complement(41284..42231)
                     /gene="whiA"
                     /locus_tag="KPB88_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG0052437.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(42248..42673)
                     /locus_tag="KPB88_000123"
     CDS             complement(42248..42673)
                     /locus_tag="KPB88_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0052438.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(42742..43848)
                     /locus_tag="KPB88_000124"
     CDS             complement(42742..43848)
                     /locus_tag="KPB88_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG0052439.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(43860..44717)
                     /gene="rapZ"
                     /locus_tag="KPB88_000125"
     CDS             complement(43860..44717)
                     /gene="rapZ"
                     /locus_tag="KPB88_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG0052440.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(44748..45461)
                     /locus_tag="KPB88_000126"
     CDS             complement(44748..45461)
                     /locus_tag="KPB88_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0052441.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(45465..46379)
                     /gene="murB"
                     /locus_tag="KPB88_000127"
     CDS             complement(45465..46379)
                     /gene="murB"
                     /locus_tag="KPB88_000127"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG0052442.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(46652..47422)
                     /locus_tag="KPB88_000128"
     CDS             complement(46652..47422)
                     /locus_tag="KPB88_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0052443.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            47740..49221
                     /gene="cls"
                     /locus_tag="KPB88_000129"
     CDS             47740..49221
                     /gene="cls"
                     /locus_tag="KPB88_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0052444.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILDSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            49539..50036
                     /locus_tag="KPB88_000130"
     CDS             49539..50036
                     /locus_tag="KPB88_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG0052445.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            50053..51939
                     /locus_tag="KPB88_000131"
     CDS             50053..51939
                     /locus_tag="KPB88_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG0052446.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            51962..53743
                     /locus_tag="KPB88_000132"
     CDS             51962..53743
                     /locus_tag="KPB88_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0052447.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(53883..55679)
                     /locus_tag="KPB88_000133"
     CDS             complement(53883..55679)
                     /locus_tag="KPB88_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG0052448.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKDNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDIEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(55893..56822)
                     /locus_tag="KPB88_000134"
     CDS             complement(55893..56822)
                     /locus_tag="KPB88_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG0052449.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(56822..58639)
                     /gene="uvrC"
                     /locus_tag="KPB88_000135"
     CDS             complement(56822..58639)
                     /gene="uvrC"
                     /locus_tag="KPB88_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG0052450.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(58898..61723)
                     /gene="uvrA"
                     /locus_tag="KPB88_000136"
     CDS             complement(58898..61723)
                     /gene="uvrA"
                     /locus_tag="KPB88_000136"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0052451.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(61742..63712)
                     /gene="uvrB"
                     /locus_tag="KPB88_000137"
     CDS             complement(61742..63712)
                     /gene="uvrB"
                     /locus_tag="KPB88_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG0052452.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNKNGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(63899..65161)
                     /locus_tag="KPB88_000138"
     CDS             complement(63899..65161)
                     /locus_tag="KPB88_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG0052453.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(65224..66528)
                     /locus_tag="KPB88_000139"
     CDS             complement(65224..66528)
                     /locus_tag="KPB88_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0052454.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(66559..67419)
                     /locus_tag="KPB88_000140"
     CDS             complement(66559..67419)
                     /locus_tag="KPB88_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0052455.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(67463..68332)
                     /locus_tag="KPB88_000141"
     CDS             complement(67463..68332)
                     /locus_tag="KPB88_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0052456.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(68316..69386)
                     /locus_tag="KPB88_000142"
     CDS             complement(68316..69386)
                     /locus_tag="KPB88_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052457.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        20560 a        14036 c        10299 g        24934 t
ORIGIN      
        1 caaaaacctt ttaaattgtc tggaaatcac ctcttcattt gttataaaaa catacaacaa
       61 atgaagaggt gattttatgg ttttaaaaaa ataattataa gtggatatgt gctttaacat
      121 gtttattaac atttgtaacg cctatatttg ttaatgaatt ataatataaa aatattaaat
      181 aatattcaac tatagattat gtaaattact ctatgaaaaa aataagttgt ataaaatctg
      241 gctactgtag attgactatt tcttttaaaa ttacaattat aataagtttt atagaattaa
      301 aaataaaact ttagagattt ttgataacat tgtagactaa atttgccacc gaaatcgctt
      361 tacagtggca aatcggtggc aaatacaata tattattagt atataatcta tataaacatt
      421 actattgttt gattatctta gctacagttt ccacatgtgt cgttactact atcggaacac
      481 attttatcaa cctcgttgct gtcatgtgaa cacaattcat tagtcctatg ccgtcctctt
      541 gcatttttcc tttgtgtttt aaatttagca ccatctacgc tattcttcaa gcctgtttta
      601 taaacaaagg tcaactgtgc agggtcaaca tttccgtttt catcaagtcc acctacaatt
      661 actttttcaa caatgctttc aaacacatag cggtcaaatt tatcaagaac ctcattctgt
      721 tccagagttt tcttaaactc tttcaaacgt ttttttatgt ctttctcatt atcagaagtt
      781 tcttgtaact tttgtctttc ttcaacaagc tgttcttgtt tacttactaa atcagcatat
      841 ttactttcgt aggtttcctt atcaataatt tcttccagac gcatatccac aagtttactt
      901 tttttcttct ctaatgcgtc tatctccttt tccgcttttg caagttgttt tgacaccaca
      961 ctactactaa gagtatcgtc cattctctgc aaaaattctt ccagaacatc tttattatca
     1021 tcacataata aacgataact ttctacaaat gcttcttcaa tagctgtttc tggaatacct
     1081 ttgctatgtt tacaaaattt ctttcctttc ttcgtagccg ttacacattg ccatataacc
     1141 ttgctgtatt ttgagccact gtgccaatta cgtcttgtta aagtaccacc acaaaagcca
     1201 cattctagca tacagctaaa tgcgtatttc ctgctatact tctcacgctt accgctgcct
     1261 accgttgttc ggttcttatt tctccgatta agaatttcct gtgctttttc aaaaacttct
     1321 tcactaataa tcggctcatg gtgatctttc atataaaatt tatcttcttc acccatgttt
     1381 tcaaggcgac gtttggaaat cggatcaaca gtaaaagttt tgcctaagag tatatcaccc
     1441 ttgtatttct cattcttaat aattccaata accgtagatg gtgcccatgt ggaactccca
     1501 tacttcgttt tataacctag actttcaagc tcctgtccaa taacagaacc accagcacca
     1561 tcaacataac ggttgaaaat atatctgaca atctctgctt cttcttgatt tacagttatt
     1621 gtcttatcct ctgggtgata atcatatcca atacaacctt gaaaacctac cagctcgccg
     1681 cgcttcattt tcattttcaa cccttttttt acattggctg aaatgttctc gacttcctgc
     1741 tgtgcaacag aactaagaac aacaagcaat aactctccat ccattgtaag tgtattgatt
     1801 ttttcatctt caaaataaac ggcgatattt ctctctttta gcatacgaac atatttcaat
     1861 gtgtctaaag tatttctggc aaatcttgat atggattttg taaataccat gtcaatttca
     1921 ccattcatac agtcatttat catacgctga aaatcttctc gttttgttac ttgtgtcccc
     1981 gtaattgctt catcagcata aatgtcagct aacacccatt ccttgttttt cttaatcatg
     2041 tcagtataat actgaacttg tgatttataa ctgtttaact gatcttcgct atctgtactt
     2101 acacggcaat atgcagctac acgcaaacgc tcaatggctt ttccacgggc ttgtcttgcc
     2161 aaaacatttt ttgcttttat tacttcaact tcctgcatag tacacctcct tattttgtgt
     2221 tcctttcata ttgttattgt actactttaa aaaacacaat tcaagcagtt atgtcagaaa
     2281 gcaccttata gtctttcatc agcctttgtt ttacaagagt gtattctttt tcagaaatca
     2341 gtttacgggc aagcagttgt tttaacatag caatttgcat actataccta attatattat
     2401 tcattaaaac cctccttgct tttttattta tggaagtaat cagacggctt cggcagctcc
     2461 actggacttt caccattccc attctgatgg gtgaaccgtg tcatacgggt gtatcattat
     2521 tcagatatac gagtcatggc agcaaccttt ccaaagatcg cttgcggttc actggcataa
     2581 tcgctcgctg ctttccccat ttcagtggtc gtggcgttcc agtccgctgg ctcgtcgtat
     2641 atcaaacgta tctgattact gtattcactt gtcaaagaac ggaaaagagt taaaatgagg
     2701 tggtagatac tctctgttat atttacttca ctttgacttc aaaccctaaa attactttaa
     2761 tcaacgctgc ccgtatcctg cctttcagtt ccatatcaag cacgatatac atattcccgt
     2821 gttcgtcata gaacgggcgt aggctggctt ttgatatgta agcgtcataa tggttcaaaa
     2881 tcttttcaat tgccacctcg ttaccgtcag ctgcagaact gatggtcgag aatgatggac
     2941 acttttttgt agacttcatc atgtttttat tcctcctcgt acatatcctt aataagcttt
     3001 aatgtgtgca gcctgtttct acatacggaa aatcgttcca tttccataac ctctgcaatt
     3061 tcagcgtcgg tcatttcaag aaagtaggac ataagcagaa tgttacgtcg gctttctgtt
     3121 aattctttga tagcttcaca taagcgttca tcatagatat ggacctctga accaaataca
     3181 tcaaatgacg taaactcaac gcaatattcg tccatggcac cgatctggtt taactccatt
     3241 tcagataaat cacagaacgg cgtttcacgt cttgcacgtc tgccaagctc tcggtcatag
     3301 ttctttacag ttcgtcctac aaccttgcga gctagacaat caaattgaag tctgatagcg
     3361 ccctcgaaag aagatggttt cataatctca ccccctttcc agcgaaagtt gctaaagcga
     3421 aaagacttct acctcttttc gcactaacac tcaccatgaa aggtgggatt tgatacccaa
     3481 ccgcaaaaat tttttgaaaa agttttatgc agtaaaaaag cacaagggca atacagacgt
     3541 actgatcttg tgctaaacaa attattcctg ttatatgctg taaaaaacca catataggag
     3601 cattatgttc cgtagaattt aaatgttttt ttaatgaact accgatggtc gaaagttgtt
     3661 gaatatgtaa gtgtttcata cggctacccc tagagccgat aaaacaagac catccagtaa
     3721 tttgtccaac gttaggcaaa aataaaacgg cggctatata accgtcgtct tattcaaaag
     3781 ttcaaaaaga actaaactaa accctctaaa gcagtatgta tttataatca gtcttgcaaa
     3841 ataacaaagt agaattgaaa tataaggtag caatccttgt gcatctaata aacttatagg
     3901 ggttacagca gaccacggaa taatattttg tatactttca gatcaatata gtccaacaaa
     3961 accaattccc gatatttagt aactaaaaag taaactagag tagaaaacat ttctttgcaa
     4021 aacacaaata ataagtaatg gactcattga caatagattg agaaatccag ttttttagac
     4081 ttagatagat tatctcactt gtaaatgatt taagttcttt gacttcatat atagcggtat
     4141 aatctgtcaa cgtgatcatt acaaaccata atgcctatat aacatacttt ccaagcaaaa
     4201 actttattct attagttggg gaatacaaaa tatttatcat tgtgcgttca acatattctt
     4261 tttgtataaa aatgtaatta caaaacacca ctcaatataa gatagataat aatttgaata
     4321 ggaattatca atctacgtaa ccattcatta cctgtcattg ttgttacaat agatttggga
     4381 ttctgtttcc attacataat ttttttatcc ttctttcaga tatctcctca ttttatcgaa
     4441 gcttcttcta cagacataca ttccataata aaatctacct ttgaatatta tttcacgatt
     4501 tcgttataga cgcgtaaaca aacaataaaa aacgaaattt cttttaagta ccataatttt
     4561 tatatcgaaa ttacaattat ccacttatat acagcattta taggtaattc aatgataaaa
     4621 aaacacatac tttactacta aattgccaca attgaatgcg ccttgatatg atagggagat
     4681 tccacaataa aaaactgtgc atctcccttg attcattcat actgatatct tatttataag
     4741 actcttttgt gtcttttttt ggtttcatgg accaaaatac tacattcatt acaataacaa
     4801 tcagcaacac aactgccata gccacccaaa agccgatatt aagaccaagc cattcggtag
     4861 ttccaaaaag ggtcatccaa agttctttcc agttcatcat tccacctcct taaagcagtt
     4921 ctccgaaatg acggacatag gcttttttta aacttgttgc caaagtcata tacagcagaa
     4981 tacatggaat cagataagca aagtatgctg caggcaatgc aacaaatcca agcatagttc
     5041 caaacacagt aaacggaata atggtcagaa ctataattcc agtcattgtt agcaaagtca
     5101 ccggagctga tgcacgactc tgaacaaaag gtagctttgg agtacgaatc atgtgaatga
     5161 ctaatgtctg gctccacata gattccacga accaccctgc ttggaacatc ccgatatact
     5221 gtgcctgaag cattgccagt tctgcaccgc tgaaatgagc aggcaaatca ttaaataata
     5281 caccattcga tacgaacaat ggacaaaata caaagtacat aaaaatatag gttgtaaaat
     5341 caaagataga actggttggt ccaatccaga tcataaaact tccaacactg gaagcgtccc
     5401 atttacgagg tttggtaata aactcctcat ccacattatc ccaaggaatt gcagtacagg
     5461 ataaatcata aattaagttt agaaatataa gatgaacact catcattggt aagaaaggta
     5521 gcaaagcaga tgcagcaagc actgagaaca tattgccgaa gttggatgaa gcagtcattt
     5581 taatgtactt gatcatgttg gcatatgtct ttcgtccctc aataataccc tgttccaaaa
     5641 ccatcagatc tttttccaat agaataatgt cagcagattc ttttgccacg tccacggcgg
     5701 tatccaccga aataccaata tcggcggctt tcatagctgc agcatcatta ataccgtcac
     5761 ccatgaaacc gaccgtatga ccattttcac ggagaacaga aaccacacgg gctttctgat
     5821 caggggttag ctttgcaaat acatctgtag actctgccac tcttgcaagc tctgcatcgc
     5881 ccatttgttc caaatcagaa ccgagaagca tattgcgaac ctctaatccc acctgcttgc
     5941 agatagttcg agtcactttg tcgttgtcac ctgtcaaaat tttggttata acaccatggt
     6001 ctttcagagc tttaatagca tccgcagtag attcttttgg aggatcaagg aatgccagat
     6061 atccaatcag taccatatca cactcatctt taacaccaaa tgcgccgatt ggggaaggat
     6121 tactcttctg tgcaatagct agtacgcgga atcccttatc gttaagattg tccactgtct
     6181 ccagaattct gtaacgaacg tcatcagtca gaggctgcac tcctccatta cactctgcaa
     6241 acgcacaaat agagagcatt tcttccacag caccttttgt taccatctgg gtcttaccct
     6301 tattatcctg taccactgta gtcagacgac gacgtgtgaa gtcaaaagga atctcatcta
     6361 ctttcacata gttttcagac aggtcaatga gacgcgaatc tgcggcttcc tcttcctccg
     6421 tcttatggat aattgccaaa tccatcaggt ttttatagcc agtctggaag taactattga
     6481 ggtaagcgtg acgcagtact cgcgtatcgt cctcgccatt tacattcaga tggtattcca
     6541 gcacaacctt atcctgggtc agcgtgccag tcttgtcagt acagagaata tcaatagcac
     6601 cgaagttctg aatggaattg aggtttttaa cgattgtctg cttcttgctc atggaaacag
     6661 cgcctttcgc aagacaggtt gttacaatca tcggcaacat ttctggtgtc aaaccaacag
     6721 caatagaaac gccgaataag aaggccgcca accaatcgcc cttggtgata ccattgacaa
     6781 agaacaccag cggaaccata atgagcataa aacgaatcaa tacccaagaa acggcattga
     6841 cacccttggt aaagcttgtt tcaaccgctt ctccagcaac tgcggacgcc atagagccaa
     6901 acagagtatg atcgccaaca caaacaacaa cagctgttgc gctaccagaa attacattac
     6961 tccccataaa agcaatgttt gtgtaatcag tcacgctttc tttctgagca cttacattgg
     7021 gtgttttttc aataggttca ctctcaccag taagacttgc ctgactgaca aacaaatcct
     7081 tcgcatccaa aatacgaaca tccgccggaa tcatatctcc agcagaaaga tgcacaatat
     7141 ctcccaccac caaatcatcc ataggaagtt cttttttttc atagtttcta cgagtaactg
     7201 tacaagtggt cgtaatcatg gctaacaatt tctctgctgc atttccgctt ctggattcct
     7261 gtacaaaacg aagcgtaccg gaaatgaata ccattgttaa aatgattaca acagtcaggg
     7321 aatcaaagtc ctctggcaca ctgccaaaca gagaaaaata cggaaaaatc atatctgtaa
     7381 ttgtggatac taaagccaga caaaataaaa tggcggtaaa tggattgata aatgcgccaa
     7441 ccagtcgttt tgccagagat ttcttttttt catgagtgac tttattggta ccatatttag
     7501 cacgacttac ggatacttca tctacttcta agccacgaag tgtggtatga agttttttta
     7561 aaacttcttt aataggattg gttgccgcaa attgaatgcg gcggttctgt tcgtcacgga
     7621 tgactgcttt ttctacagtc tgacgaatag ccatacgatt ttcttttttg ttcattggtc
     7681 ttacctcctt gaattttcat aggggcaagg aatattatat gagcgacacg gaatctccaa
     7741 attctcatat aatattctct gccttcgtgg actgacatca gagtccatgt ctttcacctc
     7801 acaatcttaa aaaaatatct atgtgaaacc tcaatgtgga ttattggttg cgatagcgga
     7861 cacttttttc gatataccag acatcaaagc cggcaacaat cagccatgcg attattgcaa
     7921 atacaaacca gtggtttgaa aatcccggca aaaatccttt cattaagaat ataatcccat
     7981 ttaacgcaat catttctcca acattacgac aaagcgggac aatattaatt ttatctttct
     8041 cctcctgcgg catatttttc cacagggaaa gatgacgata tccttttcca aaggccaacc
     8101 aaaagcctgc aagagtaaat aaaattccaa aaaagataca tgtaaaatcc atcgtttgtt
     8161 cctcctttca atgcttaaac ctgtttctta gttttgtcag cttcagaagt ttaatgaaga
     8221 gcacaataat agtaacagca agaataaacc agatcacaat actcacctga taccatatct
     8281 taaagattac cattctttgt tcaaagaaat tccaacccaa atagctacat aagattgtaa
     8341 atacagggta gaaagcaaaa agaattgctc gtttcaaatc ttcgatttct ctcctcactc
     8401 acaacacctc ccaaccagca ctaatcactt ccggaattac caatacctgc ccaacgattc
     8461 cttctaaaat attgtttttg gattttccga cagaacagta ttctgcgaca atctcaacct
     8521 tgtcaccaac cacatcaccg ctttccagat tgttcaaaaa gagcgtctta caggaattgc
     8581 tgttaatcag caataagcgt atatcctgct ctgccttttc ctgacatgta accgaaatac
     8641 gatactgctt ttcagattca tcactggaag taattgggtg aaactttctt gcaaagggac
     8701 gtagaatcag gttggaacca attaaaatag tagcggcggt gatcgccatt gcatacattc
     8761 ccgtacttgc aagaattcca atcgctgcag tacaccataa ggtggcagcg gtattcattc
     8821 cacgtactgt ccctctgtcc ttgaaaataa caccactgca taaaaaacca acaccggata
     8881 taatactgct tccgacacgg aatacctgat ccgaaccgta gagcatagga aacaaggtaa
     8941 agaaacttgt acccatgcag attaacacat tgatacgaat acctgcagga tgaccagtca
     9001 attgccgttc taatccaata agaaatccaa gtgttaagga gagggcaatt ctcaaaataa
     9061 aatctgtgta aaacatatga aacacctccc tttaactact aacagtatat aaaaaatcca
     9121 aatacaatcc tatggctaaa gtcttgcgat tttcttgcga tttggtaaga aaatagacgg
     9181 actgaaaccg aaaatggtct tagtccgtct ttgtcatatc tgatcattaa atttgtatcc
     9241 aataccccag atggtcaaaa tatatgtggg acaatccgga gcaggttcaa tcttttttcg
     9301 caacttccgg ataaaagcca taatgttgct gttatccaga atataatctt cctcccatac
     9361 ggcacgataa atctgttcct ttgtaaaaac ttcacctttg ttctggacaa gaaagtacaa
     9421 aatatcaaat tcctttggag tcaggttgac ttcagaacct tttatcagta cttttctgcc
     9481 tcttggataa atttccagat cccctgccag gattctatct gacagagtac ccactggcca
     9541 atcgctttct tctgatgctc ctaacaataa cgaagatacc tctccacgaa tcatctcctg
     9601 aataccagaa agtaaatcct tcgcacaatc actgtccggt ggatctctta gtagtttttc
     9661 taatagccag atttcaagag ccgaatccat cggaatataa ctaatattct ttttcatcat
     9721 gaatgcctcc tcttaaatca actcttgata ttttttctta taaaaatatt tggctactga
     9781 attcagcagc atatagagag ataccaccag cagtaaaaat acgaaatacc aaagaggtaa
     9841 tttcgtcagt ccaaacaacg acgcaccgcc gacaaatgta agggaagtaa aagcaataat
     9901 ccccactaga gttatagaaa tgaccggtgc agatgccctg ctctggaaaa aaggaatctt
     9961 ggaagttcgc agaaaatgta aaatcaaaac ctgtgtccac atagattcca aaaaccatcc
    10021 agtctgaaac agtgctacat actgaagttg cagggaagga tctgtaagct gcagataggt
    10081 tgcccctcca catagcatcg gacataggaa ataatacaaa aataggaagg tgacaatatc
    10141 aaacagagaa ctgatcggtc cgaaagacag cataaactgt ctcagtgttt tgccggacca
    10201 gtctcttggg gacagcatct cttcctcatc tacattgtcc caaggaagca caatacacaa
    10261 cgtatcatac agcagattca aaagcagaat ctgaattgaa gtcataggca ggaacggtaa
    10321 aaaggcactg gcacaaatga tggaaaaaat atttccaaag ttagagctag cggtgatttt
    10381 aatatatttc agcatattgg taaaagtttt tcgcccctcc attacacctt gctccaaaac
    10441 attcaagtcc ttttgcagca aaaccacatc ggcagcatct tttgctgcgt ccactgccgt
    10501 atctacagag attcccacat ttgcttcatt cagtgccgga atgtcgttga ctccatcacc
    10561 aagaaatccc acggaatgac cattatcctg catagcagag acaagacgca ccttatgtcc
    10621 cggagtaagc tctgcaaaaa catgagtatg ttccacagcc tttttaagtt cacagtctgt
    10681 catttcatcc agttgagtac cagttaagat acgctctgta taaataccca cccggcggca
    10741 aacagacaca gcaatcgctg cctggtcccc tgtcagaatc ttagggataa cttttaatct
    10801 tttgagtgca gttacagact cctttgctgt ctgctttggt gcatcaaaga aggatagata
    10861 gccaactaga gtcatgcccc attcgtcatc aggagttatt ttcttttgcg ctcccacatt
    10921 ttttcgtgcc accgcaatga ccttcattcc gtcctgtaac atttcaccta ctacagaaga
    10981 tacactctgt ctcgcatcct gccccatggg gagaagtttt ccacgatatt ctacatggtt
    11041 gcagcgtgag aatatatgtt caatatcacc tttcataata agctgactat tcccattgct
    11101 atcctgtaca agtgtactga caaacttacg agcatagtca aagggaattt catcagcttt
    11161 ttggaatttt gtcagaagat ttgcgtaatg ggcttcccgc cctggcatgg tattgcaggc
    11221 aagaatggca ttatcaatgg tattgcagac accagaatga tagcagctat tcagataagc
    11281 aagatcaaga acctctgcat tttcattccc aaaaatatcc atataatatt ccagcaggat
    11341 gctttcattg gtaagtgttc ctgttttatc catacaaagc acatccatgc taccaaagct
    11401 ctgcatggca ttaatatcct tgataatggt ctgctttttg ctcatagcaa gacttccttt
    11461 ggcaaggcag gctgtaataa ccataggaag catttccggc atcagaccta cagcaactga
    11521 cagggcgaaa gcaaaagatt ccatccattt tccatcagta atccccagta atataaatac
    11581 aatgggaact aatatcgcca taaaacgtat catcacccag gcgatggaat cggctccttt
    11641 tttaaaggaa tttttatcat cagaatccgg tttggtaaat cctccataca gggtatcttt
    11701 gcctactgcc agtacaatgc cttccccttt tccactgatg actgtggttg ccataaaagc
    11761 gaggttttca agctgagtca gtgtatcctg ctctccgtaa ctgagttttc ggcagctctt
    11821 ttcaatgatg gcgctctcgc cagtaatcgc agcctgagag ataaagagat ccgtaatctt
    11881 cgtcaatctg atatccgccg gaacacgatc acctgcagag aatagtacaa tatcccctac
    11941 cactaattct tcgccaggaa tctctattaa ctttccatca cgcctcactg tgacgctttc
    12001 atgaactagg cgatcaagct gagcagcagc gctctttgct cgcagctcct gaatcagacg
    12061 aattacgcca ctaattacga tcatggaaaa gatgataatg gcggtagtag catttctcgc
    12121 aaaattggat gctacgaaaa catccgtaac aagagaaaca atgcccagaa caaaaagaat
    12181 cacatgaaac gggttaataa atgcccgtct caaccgttgc atcgtcgtat catttttccg
    12241 cccattgaag ctgttcgctc cgtatttctc ccgcattgaa ccgatttgct caatggataa
    12301 accatccgga gatgagccaa tgtctcgata aatttctgat gttctacagt atgcgtattt
    12361 tttgatacga ctatcaaata gtgttgcttt tgacacgaaa catcccctcc tttataagta
    12421 taagtaataa cattttaaca cagtgttctg aaaatgctat atttatattc ttgctttttt
    12481 cttgcaattt actttgatat ggcatttttc agctcgatca aatccgtctc gttagggcga
    12541 gataaatcct tatcaaagtt ttcaaccatt ccttctaaat ttggagcacg gatggatacg
    12601 gcaagcattt tctcatagcg ctctcgcaca taaataggca tttccccttg acacatatga
    12661 gtgctgacaa acttcttctt ggttttcacc ttgaccatgt atcaaattta aatgaactac
    12721 ttagaaaagt tgctcataat aatatttgta ttatgagtga tagaatgaca aaagtggcag
    12781 tatggaaacc gagtgtaaaa acaatacaat tagtttgtat ggaatatctg aactacataa
    12841 ttgaccactc tgtaatttat gaaaaaatac aatgcgcaaa ttggaacggg acagaagaaa
    12901 ccaatagtat ttttaataat tccccttcac cattgattac tcttatctat tcttgcgatt
    12961 tacaacaaaa taaaacaaca taaattttaa atacgttgac tggcataatt catatgtata
    13021 gtaagtctac aattaagatg cgctttttct aatccaaata tattaagaaa aaagttttgc
    13081 gaagccatta atactttgtt taagatgtct ttcataaata taaagaataa tgctcttaac
    13141 aaaagcgtgc aaattcctaa agaaatgcac gctttcaata attatcctat aatcttccaa
    13201 ttgctctcct tatgtagcac taattcaaac tgcgatacct gtgttgcctt tgtctgatta
    13261 tcaatgaatt tcacggcaac cttgactttc acattgtccc catcaaccgt aaagataggg
    13321 tttaccaatt cagaatagag gtagtccctg ccgatagact caagcacatt tccagacaca
    13381 tagtaagcaa gctccttttc tgtggctgtt gggtatagct taaagaaagt ttccagaaat
    13441 gcagtagcgt catttatggt atccgcgtct acacttgcgt ctgcttctgg tgtctttggt
    13501 tcatagtctg atttctcgat tgctggtgca agggtagggt tctgaataat taccatattc
    13561 ccgccagcgt ccacatgaac ttttacggta taggttgcct taaccgctgt tgtctgttcc
    13621 ccctctttta tctgctgatc tacttcgtag gtagcggaaa aagtgtccgt tccagactgt
    13681 tcaatgctcc atacaatcac atctgtaatg gcagagctgg tcggtatatc cgttctaatg
    13741 gtatccatat tcaagtcctg caattccttt gtcagataac cgctgattgc ctgcgtcctt
    13801 gcgtcaatcg cttctttgct gttactccat gtgtaatagg actttgcaaa attcttcacg
    13861 aaattttcta tcccgttggt gtcctgcaag cgaagctcaa tcacttcttt ttcatgggtc
    13921 gtgtgctggt caatagcggt aaaattctta tatactccaa agctcacgct tgcaataagc
    13981 accacccaca atgcaatcac ggttttctta tgtgtgccta ccttgacagt acgtaccttt
    14041 ttttctttga cattttctgt ctgtttctta ttcttcttaa acatattttt caagtccttt
    14101 ctattgtttt actcgtcctg caccgattaa gtgctgttgc cagtaactac tacttaggtc
    14161 tgtatatcct atcgggtcgc ctgcatggta catctgatta tttcccacat agataccgac
    14221 gtgggttaca tacgaaccag cgttataggt ggaatggaaa aataccaaat cgccagcctt
    14281 tgcctgcgag agtggaagat gttgggtagc gtcatactgg gcttgtgccg ttctcggtaa
    14341 ggagatacca gcttttccat agcaccattg cgtcagtccc gaacagtcaa aggaagtggt
    14401 tggagaagaa ccaccaaaca catatttcca cccttgatat ttcaacgctt cattcattac
    14461 cttttgtgcc agctctcccg atacctgcgg aacagttaaa tactgattga ctaattccaa
    14521 tagcgatagg taattctttg aggtaatctc cgagttttcc cccgctccac accgtgcgtg
    14581 cgactttcac cgcacacggc gttccatcaa tctttttctt acttgctata tatttttcaa
    14641 gttaaacagt aataacacca ttgttgacct ttattctcaa cgcattgatt tttgatattt
    14701 gtttcttatt gagtttaaat ttgcttgtta ttttgctgat tatttcttgt gatgttgcgt
    14761 atatcagctt gtgtatagac tcatggacga taattagatt ttgaaactta tccgtgccac
    14821 cgtctgaaat aggttttata tgatgacaat ggatttcttg aaaggatagt gaaactccta
    14881 gtacagcaca tcttccaagc tgggctgtat atttagaaat tcggttatca ttatattcaa
    14941 cactcatatc taatatggga tttttcataa gttgaacaag tacccctaca tctatatcaa
    15001 gcgatttatg aattagctct cgtcctttcg gcgtaaactt acatatttca tttttaatag
    15061 atatagcttt gctgtgtttt acatatccaa tcggaataat aggacagttg tttaaccagc
    15121 gtatttgctg actttttcca tatttttttg atagatattt gttttgaata gctccttgtt
    15181 ttgttacatt taatctgtta tcaatccagt tgcttataat gtaattgact tttgaacagt
    15241 caaaggctat gagcgttgcc ttattatagt agttatgtat gcctactacc atagagttat
    15301 agatactgat ttcttttgct tgttctctgt cattagcagg gtgctgtatt cgtttaatct
    15361 gctcctttag attgttttcc gctttgttca tagctttttt actcatatgg gattgcacca
    15421 catatttatc agctttgcga accaatttca tctcaaatcc aagaaactct gatttttgtt
    15481 tcctaagatt tacgattttt gatttttcct cacttatatc taaagaaagc ctttgtttta
    15541 accattgtat aacagcatga aagattttat cagcgtcttt cctctttcga cagaatatct
    15601 taaaatcgtc tgcataacgt actatataaa tttcttttag ggttgattta cgcaattctt
    15661 tatacttatt gcctttatca attccttttc ctttatctcg cctatctaat actcgtgctg
    15721 atttactttt ggttggaatt tcttcccatt gcgaagctat ccaccagtcc aattcattaa
    15781 gtacaatatt tgatagtaaa ggcgagagtg ttcctccttg cggagttcct ttctttgggt
    15841 gttctatttt tccgtctgtt agaataatag gagcatgaag catggcacgg ataattccta
    15901 gtaaatgctt atctcgtatc cccattgtcc acatttgcct aataagtttt gcatgattta
    15961 cattatcaaa aaatgattta atgtctatat ccacaacgta ttgcaatttg ctttggttca
    16021 tcaatctgta acattgagct atggcgtgat gagccgagcg attgggtctg aacccatagc
    16081 tattgttatg gaattttgct tcacaaatag gttctaatac ttgtaggaca cactgctgaa
    16141 taattctatc ccaaatggtc ggtattccta acggacgtaa ctttccgttt ggtttaggta
    16201 tattcactcg cttaacaggt tttggagtat aacagcagaa catatcttta attttttgaa
    16261 caaactcttt ttcagacatc agttctatgt tttgtatggt ctgtttatct gtccctttag
    16321 tattactccc tttattcttt ttaatgtttc tgtatgccag catgatattt tcatctgaca
    16381 tgataacgct cataatatca acgaaaattt gttgattttg acttgcttca tatagtcggt
    16441 caaaggtttc cgtcatatcg taatattcac tatgacgaag tgttttgctt gtttttaccc
    16501 tattcatagc accagtcctt tctattagga tttggtgttt attttgtctt actcgattct
    16561 atgaagtata tagcatagtt aagtttaaag actgacttgt ggctatccct ccaccatatt
    16621 tctatggctt cttaggtact gtgccactac tttcactgac ataaatgcta ttatgtattc
    16681 ctagcaaccg tttcatagat ttatagcaat ctccccgttg tcagctttcc acgttccgat
    16741 aactctatct ttacatctgt ttttaggtgc ttgctttaag cctgcatatc tccaacgcct
    16801 gtaacactgc atggtttttc atatctacga tttttactca accacgatat gcgtccgttt
    16861 tatagaaccc gatacatttc tataccgtca tgttatagac tctttcattc gcaagttcgt
    16921 cagtaatttc attctcaccg tgaacagttt atagactccc gacctatcag ttacaccgtc
    16981 cacctctgaa caggtttcgt atatccattt tgtatattta cggtaacttt cagccgactt
    17041 caccgagctt ttaacactta tctgctataa aataaatgct aatcggagta tcagagaggg
    17101 cgtttcaagg cgttacccaa tcattccacc ctttgagtta tcagttctcc ttaaaggaaa
    17161 acgattatta cgctttcaga tttaagtgcc tgaccttttc agtcagaaac gtgtcgcacc
    17221 atagaacata tttccatagc cataccgcca gcctccgttc ttcgcaacgg ctatcgggtt
    17281 ggtataggtt actttctttc cacccgattt ctcacgggcg aagctctctg caagattaaa
    17341 agtgtgtttc tttccgtttc ctgccacata gcccacatag ccaccgccat agttatagga
    17401 ctgtatcgct acattcaaat cgtcgatatc ttgatttttg caggaagaaa gcagggacgc
    17461 aaaatactta cacccctgct tgattgagct ttccgtatct aaggaactag gcggtaaacc
    17521 aagactttct gaactctgca taacatcttc tgccgtaccg ccactttcta cttgaatgat
    17581 agccagtagt acattgacgt actcggagat accgttttct ctggcgtatt tttctaccat
    17641 agtctgatgt ttcaaaactt ctgcggataa gttcataccc gtaatgccag aagaaaagtt
    17701 gctgttttcg tcgtcgctgt ccgcactaat cagcacccca aagaaaagca ccagagaaaa
    17761 gagaatagga aacagacttc caatgatagc gatatgtttc agtttcattt tttcttctgt
    17821 cctttcttcg ttacaagatt acggattttc tctgtggtct gctggttctt ctggacagtc
    17881 tgggtctgct gaacctttgt cctacggtct ttggtgtaat tctggcgtgt ttcctttgac
    17941 acggtaactt tttctacatt ctgcctatat gtcgattggg tcgctttccc gtcaggagta
    18001 ctagaagata gcggacgttc tttgacaccg cttgtcttaa ctggttcgct tgctttcgta
    18061 gtcggagttg tggcagggcg tttgacttcc tgcatttttt ccgcacccgt ctttgaaacg
    18121 gttgaagctg tggctggtcg ctcatgggga cgggtagctc ccgttgtcgc tgatccgtca
    18181 gcctttcgct gtgcctgcct tgcttcttgt gccttttgaa gctccatacg cttgacagcg
    18241 atacttttct tatgctgttc ctgcttttcc aagcgtcccg tctgtctgga ctgctgttcc
    18301 tgcaccatac cacgcttgaa atcagacacg ctggactttg ccttttcctt tgcggaatac
    18361 accgcataag cggtctgtgt gggcatatcc ttgatattct ctttgacagc gtttgccttg
    18421 tctttcactt tatttttcgt atctaagaca gcacccacct ttgagcctgc acgctgtccc
    18481 atactggaag tggtattgcc ccgattttct ttagaagccg tattttttct ttcggctctc
    18541 ttgccagcaa cggcacttcc agctaccgca ccagccacac cgcccgaaat accgccagca
    18601 cttacagccc ttgcaatacg gtgttccata cgcctagccc gatgtcgcat gaacagatac
    18661 ggtctgcgga atatcctgcg tcccatgctt tgactgtcgc cagcgttgag agaaaacata
    18721 ctcattaagt cgcccagctt catatagata ccagcgaaac atactatctg caagaacgcc
    18781 accatgaaaa acggatagtc cgtggatatg ttataaaaca tactggaaat gctaaacgca
    18841 accgttacaa tgagcgttat tcctgcccgt gtcattatgg tattaaatac cctcacgatt
    18901 gcctgctttg ccatgctttc atagctcggt atcatggaaa gtagaaagct gataggtaaa
    18961 aacatagcga agataataaa aagtatctgg ctgaacaaca tcatacccgt aagcaagaat
    19021 acaaatatcg taataccaag attaaagaac aagaggaaga acaccatacc taaacggttt
    19081 accacctgcg gtatcgtcag attgttgttg tcgttgtcct cgatttctgt tttcacgact
    19141 tcttctctgg tctttccgtc ctcgtcctct ggacttgccg atacgagagc ttcgacacgg
    19201 tctgtcccga tttcctctgc gttgctgttt ccaaactgta agagtagcca cggctgttgt
    19261 acttgaatag aaaataggct gtcccgtatc aagtctacgc tgtccttgcc ctcgctgtca
    19321 gagttgggga gcatgatttt tgttcccaaa tcaagcgaag cggtactgat gtctgatgaa
    19381 aattcattta tcttcttgat gtagtcggga gcgtaggcaa taaacgaagc ggacagcacg
    19441 aacaccacca caaagttgat aacagcgtga agtgccttgc tggtttcccg ttttatcagt
    19501 cccgtatagg caacatacat tcccaccact aagataataa gaagcaggaa accaacatag
    19561 aagcccgtag aagaaaagcc gttctcggta acgcctgcaa gggtctgtat gctttttccg
    19621 atactgtctg ccatatcgtt aataaaatca agtttatagg cttcctgcac cacatagcca
    19681 gttgcattgc ttaaataaag gcttatcgtc cagacaaagt tggtaatgca gtaaagcccg
    19741 tactgtaccg attttccgat accgtccagc cagttccacg gaagccacga ccagctatta
    19801 tcgacgaaaa aatcaagctg gtagttggaa agcggatatt ttgaatatag attttctgcg
    19861 tttatggtat cgtccacaag ccccgtcgca tgagccaccg tccccagaag tgaaagcagg
    19921 ataagggaaa gtgccaccac gaacagagcc attttgagaa agtgaagtat cttccttttt
    19981 gtgaacgcac tctgtatcct ttctttcatc actccacctc atttctctgt acgggcggtc
    20041 tagtatcaaa ggcgtgtagt aattcttcaa agactgggtg tatctgcacc acaccgacac
    20101 gcccgtataa gtcctgcaat aaacattgtc cgttctccaa atcacgcaag cgtttctggt
    20161 tgttttcgtc gtccttgtcg ataccgaaaa attctaaggt ctgctttatc tcgttgatgt
    20221 cagtagaacg aaatgcaaac ttcaaaccga tattgttttt cagactttcc tttgacacgt
    20281 cataggcaga ctgggtaacg aaataaacgc ctgcctgcat agctcgtcca gcacgaacca
    20341 gcttatttga tagggtttcg ccttgtgcca catttaagaa cgcccacgct tcgtccaaat
    20401 ctacaatctt aaaaatgctt ctgtccgaat ggataaaatc aagggcaaag gtactaatca
    20461 caatcagcat agacaccgac aataattcaa tggtcgtgta ttcctcaaaa gtggtatctt
    20521 tatctggcag tacaaggtct gctacttgaa tgatattgag ctggttatca aggcttatag
    20581 cattttctac cgtcccgtct gaaaacagca ggtgtgcaaa gtcgtagtcc gtgaagctgt
    20641 cgatatggtc tgcgatattg cgtgagatag cggtatcttc ttttctcaat tcctcaataa
    20701 cgtgtaacag tcctctattt tcactctggg taacagaacg caccgctttt cgtaacacgg
    20761 ggaatttttc gccgtcccta gaggaaatac ccgtaaggaa tgttaagatg tcgattgcca
    20821 gactttcagc gtctttcaca ttcttcataa tcacaaatgg gtcaagaagt cctgcattgt
    20881 ccttgtcgct ggtaaggttt acgatattga tttcatgggc tatctctggg agcgtttctt
    20941 tccagttgcc acgctcactt ttagggtcta acagaagtgc ttgaccgcca aacagcaccg
    21001 cataatatac aagtagattg ttgcagaatg actttccacc gccaagactt cccacaaaag
    21061 cagaagcaag ggcattagta actgtgcctt ttactccctg cgaagctaaa gacggttgca
    21121 ggtacacatt tcttcccgta tcaacggaat agcccatata gatacccgtg ttttccccta
    21181 actgctgggt cgcaccaaaa ccaagcccag ccaagaagtc tgactttaca tactgcacat
    21241 agtcattgat atatcgcttg ctggcaggta aaaattcaga atgaagtccc aacatatcgc
    21301 cagcaggacg caccagtttt acattgaggt cgtcgtaaaa gtccttgact tcatcacaac
    21361 ggcgtttcag ctcgtcaaga tcgggtgcag acacccgtat gacgtagctt aacttataca
    21421 tactttcttt gctctggtct aaatcggttt ccagctcgtc cacgctgtct aaagcgtcca
    21481 ccacatttga gctggtttca cttcctgctt gataggcgtg attatccaaa tctttcaatt
    21541 cctttttctt gttgcggact gtcgttaatg ccttacggtt ctccacgatt tctacattca
    21601 tagaagtatc aacggggaat gtgaattgct gttgctggaa atagaagatt tcagaggacg
    21661 gaaaatccaa ctccccgaca attacattga cggtaaaata ggacacatag ctttccttgt
    21721 cctcatgttc caatcgtaaa taccgctggc tttcctcaat cacacacctt gtcggacgga
    21781 taaggtcgta gtattttatc agcgtttctt tcttcaattt cttctttggt agctggtact
    21841 cataatcttc ataggcgata ccgtccctgc cgtaaagatg ttccatgaga tacccaaaat
    21901 catggatttc caagcgacgc accttaaagc gacgggagat tttattttcc agtaactttt
    21961 ccattttcat gtaacggttg atttcatcat tcggcatgga aacaaaatca ttcatcagcg
    22021 tgtggttcac ttcatgaaga aattccttga acgtcagcca tgccgatttt ttgatgttct
    22081 tcagattgag ctgttcttcc gtaaccatga gcttaaagcc aagaaaaaag cggtagtcca
    22141 cttgattgtc cccaatcatt gatactaacg cttcggtctg ttcgtctatt ttttggactg
    22201 ccacctcacg aagtttgcct gttaccagtt tctttgactg ctcctgcata cttctaatgg
    22261 agctttctgt ggcaatctgc aacgcatgaa tttttccctc acgggactgt gcgattagct
    22321 gtcgaaagct gtcatgcacg ataaatttct gctctgcgga taagaaagaa taattgtacg
    22381 gtatcagctc ataataggcg aatacctcat tgtccttgtt ccagacaagg ttgttgtcga
    22441 tatattttat cgggaacata gttcacactc cttactgccg tgattgtttc attcaatatc
    22501 tgcttatgca gttttacggc ttttcctgca taagtgattt taggtcgcaa ggcataggtt
    22561 atctgtgatt tcaaaaagct gtacggcttc tttccgtcaa aggttttctg cgacataaac
    22621 caagtgagag caacgggaat accgaagtat ttgagaaatg ctccctcaat aatggaaagt
    22681 gggggcatat ccccaaacag aatgatgata aattccgtaa tcacaaacca tgtaatctgt
    22741 gtaaaggtaa cgggaaaggg taagttaaag tcattgattg catataagac tttttccacg
    22801 ttccagatac ccgtgtagct tttaatcttt ttcaagttct ttcagcccct ttcgtaagtt
    22861 cttcaaagag aaaaggacag caattttcag actgtccctt aaagagaaat acgctgtcag
    22921 tagcaacaat gcttgttgct gatcttccag cgttaatatg gtatctcaca gatacctcgg
    22981 cttgtttcaa tgaatcgacc acccatatcc aaatctcgcc cgtaggcttc atagtcaata
    23041 tagttctgta agttagctgg aatgtcccca agtgcgttca attcgtctat gtagtagtag
    23101 gcaacatctg tcatggtttc acaatcggga taaaaataaa tatcgtcctt atgttccacg
    23161 acttcttcca gcgtcccata gtggctgata aattcgtcca gacactctac gatatagtcg
    23221 ggaagttcct ctatcatttc atacatttca ttgagttctt caatggaaac atattcccct
    23281 atgtcgatag gaaagttgtc ggtatcatgg acagcgtatt cttcgtactg ttcattcaag
    23341 ccgatttttt cttttacatc ttcctcgtca atagggaaag tgaaccacgc accgactaaa
    23401 tatccctcat tgtatctgcc aagattagca atataaaccg ccatatcatc aatcataagc
    23461 accacctcat ttcctattct ggcagttcaa agatactgtt ttccgttaca agatagcgtt
    23521 tgttttcaag taagtgagaa gcgtaggctt caaaatcgaa atagtatgct tgacagtcct
    23581 cggataaatg tttgaatgtc gggtcgttca tcagcttttg ccttgcaaca tctatcatgc
    23641 ttttacagtc gtgataaacg gtaatcacgt tcctgcaaat ataaagtgct tctaagttct
    23701 caaacacggt aagaagttct acatattctt cctgcatatc ggctgatagc tggcggtaca
    23761 taaaatctaa ttcgttaagc tggtacacgc tggtgtgttc gtggacttca tcagcataag
    23821 gcaacacctt ttcgataatg cggtaatctt gactttctgc accgacacct aacttttctt
    23881 caaactctgc aacatctatc ggtaagtcaa accagtatga tgttgtttct tcgccatttg
    23941 ttcttcccgt ttccaccagt acccttgttt cctgcattgt tcctcacgtc ctttctgttg
    24001 tttcatcaca tcttttaagg tctggtatga cataccaaac acatatttag aaaaatcttt
    24061 caactgtttt tcttcatagt cggcagggtt cagccgtttc atttcatgcc acaccttacg
    24121 cttgtaagag ggcaggtcgg aaatgtattc acagccgact tttgcctgca tatctttaaa
    24181 aatatcgttc atttcatcac tccaatctga aatttaggta agaaaaaagc agtcaatcct
    24241 aagactaact gctttgtgtg taagtatgaa tttgtttaag tgtaactgat ttaccatgtg
    24301 tactcgtaaa gtttactgta tgtttgactt ttaaataaat atatacaatc aatcatttgt
    24361 aacttgccgt cttgttcaaa tttacttatt atcagattat cttcatagat atatgctaag
    24421 aaagtatcat actctgcata gtatttttct ctaaaaagat ttgactctct aaaatctttt
    24481 tctaaagcaa ttcttatact gtctaaagca tatttaaagt tataacaagt attaaaacta
    24541 aacttgtatg gaaaaaagaa aaataagttt ttcttgaaag taagcaattt cacataggtt
    24601 tgtatatcaa attcaatcgt tccatactca tatttctctg taagacaaga atgtaaactt
    24661 tcacaatcat aatcccgcaa cgaagcaaat aacctagtta cagtcatttg tgtatttggt
    24721 cttctcggtg gacacttacc aattacacca tcacagaatt tctgtatttg accagtaagt
    24781 tcttttgaag catgaagtct tgttgtgctt tcaagtaatt taaagtccat ttgataatta
    24841 ggtgagttac aatcacattc tccatgattt tcagatttag gtggtacata ttctgcaaaa
    24901 tttgattttt ctctaaaata tattgattta ttaactaatt ccaacaagta tttttcataa
    24961 ttacaatttt cttcatcttt aataaaattt ttgtttatga atactgcatg taaaatatcc
    25021 actctcattt catctgaaat cataagcact tttattcctt tcaaaaaata atttatcgta
    25081 cccacattat aacattctca tattacagca acaataaaat tcgttatgct ccaataatct
    25141 tattgaacag ctctaacagt acatctttca caccgccagc gttgaatact aagccgacag
    25201 caataagggc aactaccaag aagccaataa gttttgaaaa ctcacgcttg aaccctaagt
    25261 agataccgat aaccacaatc gccataagca ctaagctctg tgcatttgat aaaaaccatt
    25321 gatacaaatt ttgtccgaaa ttcatttaat tgtcctcgct ttcttctaat tgtttcattt
    25381 catagtcagc ttctttgccg atactcaaaa tacacatcaa gactacgccg atacccattc
    25441 ccatagatac cagcaggaag tcttttacta attcccacat tttttatcac tcctaacttt
    25501 ccaccaaatc ttttgcaggg gtcgtctgct gtttgattat catttcatgc ttttcactca
    25561 agattgcctg cttttcaatc gtttccatgt agtctgtacc gtttccctta tcaatctttt
    25621 tcagcatttt caaggtcggt gctacctgtc tttgtaccca acgcaatgtg cggtctaagg
    25681 tgtaaggttc tggttttgtc gtcagtttca gactttgccg attatcgcca ataaaccaag
    25741 cccagcggtc attgagtttc cagtcatttt tccgcttgtc tggttcttca tcaataaacc
    25801 gcacatactg gttgatgata gaaaaagcgg tctgctctgc gtcatagtag gtcagcaaat
    25861 ctcgtactgc atagtaagca cgttcatttc taagccgtat ctcaaaacgg ttgataatgt
    25921 cggcttcttc cagaggtgta cctaatttga catactgctc atagtccttt tcatagatac
    25981 agaaatacac atctgatttc agcgaaccaa gataaagggt actccccata tattctctgt
    26041 cgtcctctct gtgcttgata agctcgcccg actggtaata cttatagctt ctggactttc
    26101 cgatatattc ccgtttcctg catttctccg caagctctgg aatatccaaa atgcccgtat
    26161 ggtcgttgat agcaaggtcg atacgcttca taatgcctcc gtctatgagt gcgtccataa
    26221 gaaagtcata ccagcttctt tgctgtgcca gcaggtaact ttcaaactgc ctgcaacccc
    26281 gaccctttaa ctctaaaagg acaccttttt cttcgtcagc cgacgtatag ataaagatgt
    26341 cccctaatga ataatgctcc gtataactgt aatgtccgta atcttcatgg agcatataat
    26401 tgatattcag ttttaatatg tctttgatga tgtgctgtat atccagcgtg ggaaaacgaa
    26461 ttttcacata atcaaacagc atggtaagcg gtgcttctgg attgaacctt gccagttctt
    26521 tatgcaaagt ttccagaagt tcttttgacg gcttcatttt tccgctttct atcttgttta
    26581 gatattccct tgtgatacca gaagctaccg ccagcctgcc ttgtgagata ccgtaagcaa
    26641 tccgcttctc ccgtaattct tttatccatt gttcttcatt cagaaccata cccccaatct
    26701 gtcaagtgtg aagtttttta aggcgacttc acagccgatt tttgctagga aaccatgcca
    26761 gaagccttat gtttcctatg tttttttgtc tattgtctat ttgcaaacgt acccctctgt
    26821 tagataccga ggggttttac ctgctggcgt ggcttacgcc acaccagcaa cggctagtcc
    26881 gagccgtcgc ctttcgcttc gcacgtcgcc gtcccgtcct gccttgcttg tgcaagaaaa
    26941 ccgattgtct gcaaaaaatc atgtcctttc gggactaaag gcgtgtaaaa ttcacttatg
    27001 acgcttgttc ccacatcaca atagccacgc cctttgatac gcttttggaa aaactgcttt
    27061 ttcacatctg aaccaaacag cataccgtaa cctaactcgc tgatacgccc caatcccaca
    27121 cgtaaattga agttatctct gataccgtcc gagaaatatt ttgcgtctgg acgctggcag
    27181 gcaacgataa ggaaataacc tgcttgtcgt cctaacatga cgattttctt taactggcta
    27241 agtaagccca cactttcttt tgtccccagc atttcaaaaa acgccacata ttcatcaaag
    27301 ataagaaagc agggtggcag tcccagatag gcgtaatttt cgcccgtctt atagttcggg
    27361 tgtcgtttca tttgctcgct tcgctgtacc atgccctcat aaaaagcatt gacacagtct
    27421 atcatttctt ctttggtgtg atacacgttt cccatgactg ttcccaagtc tgctaagtcc
    27481 gcatttttcg ggtctaagat gtaaaggaca gcgttggtat gcagtagggc ttcaatgagc
    27541 gtcagaagaa aatatgtttt accgccacca gtcccaccag caatcagagc gtgagggagt
    27601 gcgtcatatt cccagacaag atttttcatc agtctaagac agccgttctc tgcccgtact
    27661 tcatcaatgg taatgcggtt cgctatcata tcataaagca gggtatattc gatatagccg
    27721 tcatgcaggg tcttgtcggt cagctcacaa tacaagccac tttccaattt atcctctaac
    27781 cgtaaaagct ggtcttgata tttccccagc gtaatttcac agcggatatg aagcagtccc
    27841 ttttccattt gataataaat ctttggaaac cagacgattt tttcccttga tctgctttgc
    27901 aggtcagtaa aaaaaacgct gtcttgtacg gtatcagctt cataccactt gttttccagt
    27961 atcattcgtg ccagcttttg acggtgcagt agctttttga aactgtcgta acagaagcgg
    28021 taatacagaa aagcgaccaa tgcacaaaca ccagtcgcta tcagtatggt tatgaagttg
    28081 taaagggaaa gcgtaaagcc attgtctaat aagctgaaat gttcccagtc ggtacgcatg
    28141 agctgttttg tatttaatag caggataacc gctacgaaca caaacaggag tgtccccatt
    28201 gaaaagcgat agacaaggtt cttatcgctg gctctgatac ggtgtccttt gtttaaaatc
    28261 attctcataa atcaatcact cctttctggg tatgaaaaaa gcagtcaatc gtatagacta
    28321 actgctttgt gtgcatggat atgaagttgt caaactggaa tttaacgttc actcatatat
    28381 tgttttattt catcagaagt caattttcgt acttctgtat gtggatattc tttatactct
    28441 ccaacattaa acacaggtgt catttgtttg catttcttgt ctttattctt tttcaagaat
    28501 agatgtaaat cctcattccc ggcgaatatt ttataagata ctctgccttt actgttctct
    28561 atctcataaa taccacatga acttttcgtt gtataatcgg ctgttcgtaa ataaagtttt
    28621 gcaatttcca atgacttaaa aggaaaattc catctttgta atcctctgtt tgggtcatgt
    28681 tcaatacaaa tgcaacgtcc acaagttcca caaatatatt gagtgtggtt ttctaagcag
    28741 tccaagggat ttagtaatgg tgttattcta ttttcatcag agtaacattc ttcacacatt
    28801 tttcttttcg ctccttatca aattctaatt tgtcgctttc ttcataccca tattataaca
    28861 gttccgtata tcctctacaa cgaaaatcta ttcagcaagt ctattttttc ggttgtccct
    28921 gcggtgtatg gttctgtgaa gtgcctgtgt tctgattgcc tttattcttc agtacaatat
    28981 cttctgcctt tacataccag tccacatctg cacccgtata ggtttttcgt gataccgtat
    29041 cggctacggg attgacaagc tccacgactg cattgtaagg aaattccctt aacggtactt
    29101 ctggcggtac ggacacgggg ataattccac catgcagact gcacttcaaa tcatagatac
    29161 gctttttaag ctgggtactc ggtgtcccgt cctcattttg caagaacaca tcacgcaccg
    29221 ctgtaaattt taattctcca aatgttttct ctttgtcaat gacaaatcca tttgataatc
    29281 tcataaattc ttcactcctt tacttttctt caactgctac aatatcatca gctactaata
    29341 cataatcggt atgtcccaca tccccgattg cgtaccctct gccgtataac ttcggattga
    29401 caagttttac tttctgttca tacttgaaat gcttttcgcc agcctgcacg ggaatttcca
    29461 ccacaacatt ttcgcctttc tgtacgtcag aataaaggtt atagcttcgt ctggctatga
    29521 ccttgcggtt gtttttatcc ctttcaaaga taggctcgct ttcgcctgca aattcaagag
    29581 ttccaaaaga ctgtgccata tctggcacga catatttcat ttccatattg ttttacctcg
    29641 tttctttcta tatttgataa gtttatgatt gtaatttttt attctggctt gtctgccgtt
    29701 tgacagtttc ggcggagaac gtgatttttc tttcatcata ccgttaccgc cttatgattt
    29761 tttcattgta cggcgtttgt aaagatatgt tccagccagt ccaccagcag atgtaagcaa
    29821 catcacaaga aatgccatga catttgtact gtcgcccgtt ttaggactgt cgctaggtct
    29881 gtttggcttt tctggtgtct gcggtgtttc gagtttctct gggacttctg gtttttcatt
    29941 aaaagtaatc gtctgtccct cgtcctcaat atccttatgc tcggtaactt tcttcggctc
    30001 gtctggattg cttaaatcat acaattcttc aaaagttaca agctgtttgc cgtcaaggga
    30061 agtagcgtca aaggtaaagg caacttccgc tttcatagtt tcgctgtcag cggtaaaggt
    30121 gtaatcactt tccacacgtt tcccattgat aagaagttct gcattttctt ctttcaacat
    30181 ctgccagcct acaagtttgt actgtgtacc gacttccaag ccctctaagg ttacggtatc
    30241 aatgattgta acgtcttttc cagcttcaat ctctttgttg ccgtctttgt cggtagcagt
    30301 cgtatgtatc ttgatgatac gttctgtaat caatacagtt tgtccgtcgt cctcaatgtc
    30361 tttgtgttcc gcaactttca cgggttcgtc tggattgctt aaatcgtata attcctcgaa
    30421 tgttaccaga tttttaccgc caagctcgga cgcattgaat gtataagaaa tttccacttt
    30481 catttcttca tcatcagcga taaaagtata gtcattttcc acacgttttc cgtcaatgat
    30541 aagctctgca ttttcttcct ttaacatctg ccagcctttg agctgatatt ttgtaccttt
    30601 tgtaagcccg tctaatttga cagtatcgac gattgttacc tcttttcctg caaggatagt
    30661 cttttcgctg tctttgctgg tcgctgtggt atggatagag atttcttttt cgtattcatc
    30721 agtcaaagtc cctaaatcaa tcacaaggtt atttcttgat accacgattt caaaaggtgg
    30781 gataagttca aagcctttgt tactatcgca acgcatttct tcaatgatgt aagtatcata
    30841 aggcaatgca cctttgctgt cgtctgcttc agaagttcca aaccacacac cgtcctcgct
    30901 ggtcttgcct gcgttagtat tgtgcttatg agaagtccat tcagcagaag tggagaattg
    30961 cccgttatca tcagtaacta caacatgact ttctcccgtc gtcttgcttg tgatcctaaa
    31021 gggaacatca gcaagacgct tgtgtgtgcc tgcaccgatt ttcactccct caatatcccc
    31081 acgcttaatc tgattgtaga tagagtgggc ttcgtcggtt aagtccacga tttttccatt
    31141 ctctgtgatt gcaaagtcaa tcggcttcgc accgtctgtc aagtaaccgt cgggagcttc
    31201 actctcaacg atacggaatt tcccataagg taaaaggtca gcagaagtag aagccacacc
    31261 ctcaatatct gtacgaattg tctttaccac ttcatttttc ttatatagct tgccctcaac
    31321 caaaacagaa ttgtcattta aggaaatgac gtcaaaagca gtatctttca aagtggcact
    31381 tccttgtggt ttggtatcgc tcgtttctaa atctcttttc tgaattttca caccgccacg
    31441 gataaccttg tctgatacgg aaaactggtt acttcccgtc aatacagcaa ggtcgccgtc
    31501 ctctgtaatt tgtgtaaggt ataagccttt tatctgttca gacttatcgc cagcctgcat
    31561 atatgcaccg tccagcaagt agccgtttgg agcttttgtt tcctcaacag atagtgttcc
    31621 taaaggaaga accgctttgc cgtcctgcat atagaagctg tcgccagata ccttgtaagc
    31681 gtccgctaat tttgtgatgt agtgagttgt cccgtcactg tctgtttcag cgattgtctt
    31741 tgtaacccat gtacgagtag cttcggcagg gagattgtct ttgttataga agccagcata
    31801 atacttccat gtaaactctg caccctctaa agaagcgttt ccctgcggat tgtctttctg
    31861 cgtttccatg tcaatcttga aaagctcaat caaggtgtcc gttacttttg gtgtatctga
    31921 tacgttcaag gtcgctgtct ttccagcttc aacttttaag gaatacacgg ttgtatcaac
    31981 tttatatcct gctggtgcgg ataattccct aatatagact gtacctgctt ttacctctac
    32041 aacatctgta tttccgtttt tatcagtcgt aagggtggca agctgtttcg tgcagtcctt
    32101 atcagaaaag acaccatagg tcgcaccagc gattgagtaa ttcccgttac cgtctgtaat
    32161 gctggtatta ctggaagtct tttgcagttt tgcatttccg acatttaact tcgcccagaa
    32221 ttgtcccaat tcctgcccct cgccagagta gatataaccg ccacattcat agcgtccttt
    32281 attttctttc acaaaggctt tagcaccagc gaaaacttcg tcctgagttg cttttggtat
    32341 ttcatcataa gaagctcgca cgttgtcgca ttgccagcca agatgtacgc tcaatctctg
    32401 ccagacaaca cattgttcca acagatagac ttgcttgtag ttcaattcct tgtgtgcttc
    32461 gccatactgt ttgacatact ctaaggataa cgccacatct gaaatctggt cggcactcat
    32521 acgggtgctt gcgtcagctc tggtcttgta gccattctta aaatctgtat tgatgtctat
    32581 acagtaagcg tcctcgccct caacagtcaa atgtccctcg ttaaaggtag aaccaatcga
    32641 accgtcattc attacttttt ctacgatacc gacacgttcc tctgattccg tccagtattg
    32701 cttgctttct gcatggacgg cggtactcgg taaagcagta acgacagtcg caaaggcaag
    32761 tactcccgta cacaatcgtt tcaatatctt tttcataatt ctaaatgctc ctttcatttt
    32821 gggtaaaaaa atagacgctc aatattgaac gtcttacatt gaaaagggat actttttaca
    32881 agtacccctc aaatctaata tttaattgtt tgtgttccca cactctatat caacaactac
    32941 tgaaacattg atatagtttt ttatctcttt atttctgatt ttatcgccct cgctcgtaaa
    33001 gactatatgc ttaaaaaagt gcctaaaatt aaggatttct acatagttcg tctttgtagc
    33061 aacacacaag tctctatatg tgtagaatga gggaacatat ctacaggttg tgcctcaaca
    33121 catttaaatc cgtgaacatt tagatagtct aaatcccttg ctaaagtaga aggattacaa
    33181 gaaacataga caattctttc cggattcatt gaaacaatag tctctaatac cttttcgtca
    33241 catccctttc taggtgggtc tacaacgact acattggcag tcttaccctc tctatacatc
    33301 ttaggtacaa tttcctctgc ctttccaaca taaaattcag cattatgtat attgtttaat
    33361 ttagcattta ttttagcatc ttttatggca tcatctataa tttctattcc ataaaccttt
    33421 ttagcctttt gtgccaaaaa taaagatatt gtacctatcc cacagtaaat atcaaaaaca
    33481 gtatcagtat catttaaatt tgcatattca agagccttat catacaatac tttagtttgg
    33541 acagggttta cttggaaaaa tgataatggt gaaatctcaa acactaagtc acctatataa
    33601 tcgtttattt ttccatctcc atatataact atattttctc ttcctagaat agcattcgta
    33661 tctttttgat ttacatttac aaccaaagtc ttaaatccag gtatattttc cttaagcact
    33721 gaagctaatt ctttaagata tggaagtttg tttccatttg ctactaatac aaccattact
    33781 tctttagttg taaaacctat ttttgttact aaatgcctaa tcaaccccgt gtgagtcttt
    33841 tcatcatata tacttacctt ataggccctt atatatgttt ttattatttt aacaatttta
    33901 tcatttatct catgttgtat tacgcatttt tctgtcgata ttacatcatg agtcttcttt
    33961 ttatagaagc caataattgg aataccatta ttcttttgta ttggaaattg tgctttattt
    34021 ctatatctat atggattatc cattcctata gtgtcatgaa cttctacatc ttctaactta
    34081 cctattctcg aaagtacttg tttaacttca tttgttttca tttcaagttg tttattataa
    34141 tctaagtctt gtatctgaca ccctccacaa tccctaagat tgtcactaca cactcttttc
    34201 accctgaatg gagacttttc tattatctca acaatatctc ccacagcata atttttcttt
    34261 actttattaa tcctaacttt aactttatca tgtattaatc cgccctctac aaaaactgta
    34321 ataccattgt actttcctac tcctactccg ccttgaccaa tgtctactat atctacaata
    34381 tacatctcgt ttttcgaaag cataaatcac cctccaatca atctattata tttaaatctt
    34441 tacaaacttt tttctttaaa tttatattaa aatattgcta ttaattaaac tatatttttt
    34501 actaagctac aatattttat tttaccaaaa aaaaaggagc ttataaagcc ccttagtaaa
    34561 taaatttatt atttttctta taaaactcta gttgaacctc tatataccac acctcttgat
    34621 gcatccacag ttacaacttc tccatctttt actagatttg ttatatcagt tgcagacaca
    34681 ataactggta tatctaaatt tatacctaca atagctgtat gtgatgtcat tccaccattc
    34741 tcagttacta cagctcctgc tttttctata taattgttca tttcagcatc agtagcagta
    34801 gaaacaagta tgtcgccttc attaaagtct acacaagcat ttccattctt tattatacga
    34861 actttacctt ctactgtttg agtacctaca cctatacctt gcacgatttc ttcgcttatt
    34921 acatgaacct ttattaaatt agtagttcca cttactccta ctggtactcc agcagttata
    34981 actactaatt ccccattatc tatataatca gcttgtctag ctgcctcaat tgccttctct
    35041 ataacttcat ctgtatttcc tgcaacagaa gacttaactg gatatactcc ccatgtaaga
    35101 gctaatttat tcatagtctt ttcattattt gtagtagcta ttattggaga ttttggtcta
    35161 aatttagata ccattctagc agtatatcct gaagaagtag atgttattat agctgatgca
    35221 tttaaatcta ctgctgttgt acaagttgca tggcttattg catctgttac agtaacattg
    35281 ttagtaccat tttcctttag taatctatca taatctaaag tctcttctgt tctttttgct
    35341 attgtagcca tcatttttac tgcctctaca ggatattttc cagcagcagt ttctccagat
    35401 aacattatcg catctgttcc atcatatata gcattggcaa cgtctgtaac ttctgctctt
    35461 gttggtcttg gatttcttat catagaatca agcatttgag tggcagtaac aactggcttt
    35521 gctaattcat tacatttttt tatcatcatt ttttgaacta ttggcatctc ttcagttggt
    35581 atctcaacac ctaagtcacc tctagccacc attataccat cagaaacttt taatatttca
    35641 tctaggttct ctacaccttc ttggttttct attttagata ttatttgtat atcagttgca
    35701 ttattgttct ctaatacttc tcttattgct agtacatcag atgcctttct tacaaaagaa
    35761 gcagctatat aatctattcc ttgacttatt ccaaactcta tatcacttat atctttagga
    35821 gttattgctg gtaaatttat cttaacacca ggtacattta ctcctttatg atttttaact
    35881 attcctgaat tttcaacaac acaaactata tcttctccat ttatttcttt tactcttaag
    35941 ccaactaaac catcatctat tagtatagaa tctcctgatt taacgtcttc agcaagacct
    36001 ttatagctta ctgtacacat ttctttagtc cccataatat ctttcatagt tatagtgaac
    36061 ttttgccctt cttctaaaaa tacctctggg tcttcaaagt tacctgttct aatttctgga
    36121 cctttagtat ctaataatat agcaactggc ttatttaatt tttctctaac tttctttgcc
    36181 atatcaattc tttctttatg ttcttcatgc gaaccgtgag aaaagttaaa tctacacaca
    36241 tttagaccat tttgcataag ttgagttaaa acttcctcac tttgtgatgc tggacctagt
    36301 gtacaaacaa tttttgtctt ctttacatta tttaacatat ttgctcctcc tgagatatta
    36361 ttccacagat ttatttttaa tactaaaact tatatagata atatttttgc catctcataa
    36421 gcttctttat caaatacttt cttttgagct aaagcttcac ttatttctaa atcaattatt
    36481 ttgttttctt ttattcctac aactctagct gattttccat ctaataataa ttctactgct
    36541 cttactccaa gtctacttgc tagtattcta tcagatacag ttgggcttcc tcctctttgg
    36601 acatgcccca atacagtgac tcttaaatct gctcctgact cttttttaag ttccttttct
    36661 aaatcactag cacttccgac tccttctgct aaaacgatta tactatgtct cttacctctt
    36721 ttttgagtag tctttaatct tagagcaact tcatctactg taatttctac ttctggtact
    36781 attatagttt ccgctcctcc tgcaagacct gcatataagg ctaaatctcc acagtgtctt
    36841 cccataactt ctactatgtt aactctttca tgagatgatg atgtatctct tatcttacct
    36901 atagcatcta taatagtatt cattgcagta tcaaatccta tagtataatc tgtatatgct
    36961 aaatcgttat ctatagttcc tggtatgcct atagctggga atcctaagtc actaagtttt
    37021 tgggctcctg caaatgaacc atctccacca attacaacta aacaatcaat tttatacttt
    37081 ttaagaattt ttactgcttt taatctacct tcttctgttt taaattcttc acatctggaa
    37141 gattttaata tagttcctcc cctgtgtata atatctccaa cagaagataa gttcatttca
    37201 gttaaatctt cttctagtaa tcccttatat cctctattga ttccatatac tttgcatcca
    37261 taatatattg ctgatcttac aaccgctcta atcgcagcat tcattcctgg agcatctcct
    37321 ccgcttgtta gtaatcctat agttttcatt ttttacctcc ttggatttta tgtcaatttt
    37381 ttattataat cattgttatt gtattctaat ggactttttt tgtccctata tcacttttta
    37441 atcccatcaa gactaaaaaa attaaatgtc cttaaatgtc cattttgtga tattatatca
    37501 caaaacggac aatatttttc atattattat aatatattta gatttttata taaaaaatca
    37561 agcttttact tttatgttat ctttaggaag taactcttct aagttattta taaattcatc
    37621 acatatacaa gtcaatgccc cggtcatttt atatgcttta cgtttaccat ttatattttc
    37681 atccataggg tataagtata tactgtcatt acctggatat tgtttggcta tttctacaat
    37741 attattagtt gtatcagtat cagacatact atctattttc aaatacaatt ttttaccact
    37801 ttctggctta atattttcta catttctagt tcttcctgaa ttgtagtttc tatctgcaaa
    37861 gtcagtatca tcatttatat cttttatctc tctagctata agttttgcat tttcgccttc
    37921 ttttatactt aatgttcctt ttacatatat tatattatct tcattcaaaa tgacattata
    37981 cttttgtaat aattgaggaa aaactattat ttctatagca ccatacaaat cttctaattc
    38041 aataaatgcc ataatttcat ttcgttttgt tgttttaata gttttagtta caatcattcc
    38101 acccattata acatctgttt cattcatttg taggtatgac tcttcttcct cttttaatga
    38161 atttaatttt ccattatcca tagatgtttt ttcttgtagt tctttttcaa attgtgataa
    38221 aggatgtcca cttacataca ttcccaagac ttctttttca aggttaagtc tctctctttc
    38281 ttcatactct tgccttttag gtaaactgta tatttgttga aaatcactat cttcctcact
    38341 atctccaaaa gcatcaaata aagaaatttg accttgaaca tttttctttc tatccataga
    38401 tacactttct aataaacttt cataaccagc catcaaacta gctctatttt cacttatatt
    38461 atcaaaagct ccacatttta ttagactttc tattactctc ttatttgtat ctttttggtc
    38521 taatcttttg actaaatcta ctaaatctac aaataatcca ttagattctc tttcagcgat
    38581 tatattgttt attatattta cgccaacatt ttttacggct gctagaccaa atcttatatt
    38641 gtcgccttca actgaaaact ttgaaaaact tttgtttata tctggaggta atacatctat
    38701 ttctagtgct ttacattctc ttatatactc aactacctta tcagtatttc ccattacact
    38761 ggttatcaat gctgccataa attcaactgg ataatatgtt tttagataag ctgtctcata
    38821 tgctaaaacc ccatatgcag ctgcatgtga tttattaaat gcatatctag cgaagtctat
    38881 catatcatca aatattttat tagctatttc ctcttctaca ccatttctga tacaccctgc
    38941 aatttctata ttaccatctt catcaaattt accatgaata aaatattgtc tttcttcttc
    39001 catcacatcc atctttttct tactcatggc tcttctaact aggtcacttc gtccgtaact
    39061 ataaccacct aaatctctaa cgacctgcat aacctgctct tgatatacca aacaaccata
    39121 tgtaacttcc attataggct ttaatttttc atgtagatat gttacatctt ttgggttgct
    39181 tttatttttt atatatgcag gtatagaatc cattggacct ggtctaaata gggatattcc
    39241 cgctacaata tcttcaaaat tgtctggttt tagctgtttc ataaaacttc tcatgccagc
    39301 actctccaat tgaaacaccc ctagtgtatt tcctgatgaa agcatttcat atactttcgg
    39361 gtcgtcataa tccatctttg aaaagtctat tttttctgtg tagtttttta taactctatt
    39421 tttttctatt aaatcaagag tatctcttat aactgtaaga gttcttagcc caagaaaatc
    39481 catctttaaa agcccaagtt cttcaagtgt tgtcatagta aactgtgttg tgattgcatc
    39541 ttgatgtttg tacaaaggta catattcatc tacgggattt tttgatataa caactcctgc
    39601 tgcatgagta gaagcatgtc ttagcattcc ctctatctgt tttgatatat ttattatttc
    39661 ttttgtttcc gggtcttggt catacaattc tttaagatta ggatttgtat ctagggcctt
    39721 atctattgtc attcctaaag caaatggtat ttccttggct accttgtcaa ctttattgta
    39781 gcctatatcc aatacccttc caacatctct tattgcagct tttgcaccca ttgttccaaa
    39841 agttattatc tgggctacat ggtcatcacc atattttctc tttacataat ctataacttc
    39901 ttctcttctc tcataacaaa aatctatatc tatatctggc atggatatac gttctggatt
    39961 taaaaaacgc tcaaataata gagagtactt tattgggtct atatcagtta ttttaagtgt
    40021 atatgctact atcgaaccag ccgcactacc tctacctggt cctactataa tattattttc
    40081 tttagaaaaa tttataaaat cccaaacaat taaaaaatac tctacataac ccattttttc
    40141 aattacatct aattcataat ttaatctctc tagtatctca ttgctaggat tatcatatct
    40201 ctcttttaat ccattaaaac acaactccct taaataagtt tctggtgtat atccttctgg
    40261 aacatcatac ttaggaagat gtatagtatt aaaatcaaac tccacattac acatatcagc
    40321 tattttcaca gtattgtcaa tagcctctaa tgcatatgga aataattctt ccatttcttc
    40381 tcttgatttt aaataaaact catcactacc aaatctcatt ctatttgggt catttaatgt
    40441 ttttcccatt tgaatacaca taagtacatc atgtattttc gaatcttctt tatttacata
    40501 gtgaacatca ttagtagcaa ctagtggtat accagtctct tttgataatt ttactaaacc
    40561 agagtttact tctttttgct caggtaaatt gtgatcttga atctccaaaa agaaattttc
    40621 ttctccaaat atgtctctgt agtcaagagc aaattgtttt gctttttcat aatttctatt
    40681 cattaaagca cgtgctacat ctcctgcaag gcatgcagaa agtgctataa tccctttgtt
    40741 atgtttttta agttcttcca tgtcaactct aggcttatag taaaaacctt ctacgtatga
    40801 agttgaaact atttttatta aatttttata tccttccata ttcttagcta acagaactaa
    40861 atgtccttga tatttgtcat agtttgggtc tttatctctc aatccacgtg cagctgtata
    40921 gacttcgcac cctataattg gctttattcc ttcttttata gctgttttgt agaaatctat
    40981 aacaccaaac atacaaccat ggtcagttat agctattgag ctcatgttta gttccttagc
    41041 cctttttata agttttttca ccctcgaaaa cccatcaagt aaactatact cagtatgtac
    41101 atgaagatga gcaaaatctt tgttcattaa atcacctcct gctttataat tttaatttta
    41161 ccacaaatat atgtactaaa acaaaaacag agacttttat ctcaaaatag gaattaactt
    41221 caacgtatac cattatatgt tataaattaa tgcttattct gagattgtcc cctcaattta
    41281 ttgctatgat ttttttttca aatctgttgc aatttcttct atttttctaa gtctatgatt
    41341 gactccagat tttcctattg gtggttctaa catctctcca agttctttga gagtcatatc
    41401 ctcatactcg attctaatct tagctatctc ttgtaaattt tctggcaaac tgctaattcc
    41461 tatagtctct tgtatgagct taatattttc tacttgtctt actgctgcat ttactgtttt
    41521 agaaaggtta gctgtctcac aatttactat cctgtttaca ttatttctca tttcttttac
    41581 tattttagta ttttgaagac ttaaaagagc gtggtgtcct cctataatac tcaacaaatc
    41641 tgatatttgt tcactctctt tgatataaac aacgtgatta ttttttctag ccacaatttt
    41701 actattaaaa cctaaagagt ttataagttc ttttagagaa tctgcaaact cctcattatt
    41761 agttacaaat tccatatgat agttcttttc tgggtcgctt atagaacctc caccaagaaa
    41821 agctcctctt atgaaagctc tcttacatcc ttcatgctta attaagttct ctgaaatttt
    41881 gtttatagta aaaaatcctt ctttatcttc aagtatacct aactttttta ataacatttc
    41941 tgaacccatt tcgcttgtta ccattaatac ataactgtta tttctcttta gcatttgatt
    42001 cttatttact gagattgtag tattgatatt aaaattcttt tttaataatt taaatacttt
    42061 acgggctatt gaatttaact ctgttgttat ctttagattt agcttcttat atccaacaat
    42121 ttgtatactt ccccctgatt taactagagc tgacaattca gctatattac aacattcatt
    42181 cccagatata actcttgcta gttcattttt ggtttcagtt gaaaaagaca tatctatcac
    42241 ctattaacta atttttcttt aacctttttt ttatttcttg taaggcaacc tttattatct
    42301 ccttttcatc atcataccta gctaagtcta ctactctatc taggtggata aatttagcat
    42361 caataaagcc ttcctctttt tgtggaatag tatccatatt ctttgcttgc atcaaatacc
    42421 aaaaaactgt tttatgaact gctctatttt catcccagtt ttctttgaat gtatagtgta
    42481 tttcacctaa atatttcaat atatctgctt ttactccagt ttcttcactt acttcacgta
    42541 atgctgcctc ttggttattt tccccttctt ctacttttcc cttgggtaac acccaatctc
    42601 cattgaactt ccttagtaaa agaatcgtat tgccaaacag gactacacca ccagcactaa
    42661 cctcttctct catcgatgtt cactccttaa aactaacata atgataagga tttctacaca
    42721 attttttaaa atcctttttt actaactttt gttgtaatta tgatttaggg ctaactcaat
    42781 aactatgtta gatatatact ttgcatcatg tcttatataa ttatttttta tttcaattaa
    42841 attcttctct acagttttta tacccatctc ttttaattta tctttttgct ttttatctaa
    42901 caagacttgc tctgctccat cttgtttata taagtcaagc atttcttctg gcaatatctc
    42961 attatttgcg attacataat ctattaaatt ctcatcagtg tgttttacta tagcattaac
    43021 atgctctaat acattgtacc cttctgtctc acctggttga gtcattatat ttgctatata
    43081 tacttttgga gctacagacg atttaatagc gtctacaatc ccctctacaa gtaaattggg
    43141 tattatactt gtatataaac ttccaggacc cataattata atgtcagcat cataaataga
    43201 tgcaataaca tcctttaatg gttttacatc ttttggattt aaaaaaattt tatcaataga
    43261 gcttttttga atttttgact cttcaggtat actagattct ccatttatta tattgccatt
    43321 ttctaatttg gcaactaaat ttacatcttc caaagttact ggtaaaactc ttcctgttat
    43381 agcaaaaatt tcacttaatt tatatacagc tttttcaaag tttccataca acccattcat
    43441 agcagccaaa aacaaatttc caaaactctg accttttaaa agcccttctg taaacctata
    43501 ttgcataact tcattcattg ttggctctat atttgcaagt gctaataaac agtttcttat
    43561 gtctcctggt gggagcattc ctaagtcttc tctcaataca cctgaacctc caccatcatc
    43621 agcaacagtt acaattgcag ttatattttg cgtagtgtgt ttaaggcctc ttaagaatac
    43681 cgattgacca gtacctccac ctatcacaac cacatttggt tctctatttt caacaataga
    43741 cctttgaaat agttttactt tttcattttt tgctttttta tatataaata acgatacaat
    43801 taatgcaatc caacctaaaa ttcctacaat aataagtaat ttagccatgt ttgcctccat
    43861 tatcgcaaca tagagtctct atgtttcttt acaactctat atcccttatt agataaatcc
    43921 tccgctataa gatttgttat agtaactgac ctatgtcttc cacctgtgca tccaacacca
    43981 attactagat gttgttttcc ttcctctata tattgaggta ctaaaaaatg aatcatatct
    44041 aaaagtttca cataaaattc ttcactgatt ttagaattca taacataatc tctaacttct
    44101 ttatcatcac cagttttagc tcttaactct tcaacataat agggatttgg aagaaatctt
    44161 acatcaaata ctaaatctgc atcagcaagt ataccatgtt taaatccaaa agatactaca
    44221 gaaatagtta ggtttggatt atcttcacct gaagaatata ttttctttat ttcttcctta
    44281 aggtctttag gtttcatgtt agttgtatca attatacatg tagataactc ctttaaaggt
    44341 tccattatct ttctttccat ttttatacct tctggaatct gcatatcttt tgccaatgga
    44401 tgatttcttc ttgtcatttt atatctcttt aacaatacat catcattaca atccaaatat
    44461 accatttcat attcataatt ttctttttct aaataattta agctttcatg aagcgcttca
    44521 aaaaactttc tccctcttat atctatccct agagcaactt tatcaatact tgaatttggt
    44581 tgataacata attctgcaaa tttagttatc aaagcaggag gtagattatc tacacaataa
    44641 aatcccatat cctctaaagc tctcattgtc tcactttttc cagatcctga aagacctgta
    44701 actataacaa atttcattgc taactcctcc cttttatctt atgtttatta tattataatg
    44761 ttttctacta acgatatatc cagattagag tcagatatag tcttcatagc tagttcaaaa
    44821 tcaccaactc tttctggaca atgtgcatct gagcctatta taaatttatt ttctagccct
    44881 tttattttat ttaattgatt tacattcaaa aaagaatggc tactatttat ctcaagtcta
    44941 gtatttgtct tttttgctgt ctttgctact tcttctatat acacatcgcc tttatctcct
    45001 ggatgagtaa ttataaaaat atcattatta cttatcgcat ttattacagc ttttgtatta
    45061 tattcttttg ctttttcagt tttaaaaata tagttattac aatgatttaa taagcttctt
    45121 aatgatgttg gagtagaacc aaaatgatat ccagccataa tataatcaaa atctctaata
    45181 aatttatcat ccatatctat atttccttta tcatctaata tgttacactc catacctaaa
    45241 agtatattta tatcagtgta cttatcattt aaataatcta cttcttctct cattttgaga
    45301 atatcttttt tctttacacc atatgcaata tgtttataac tatggtcaga tattcctatt
    45361 gtttttattc ctttagaaat ggctaccttt acattatcct caatagtccc tttgccatga
    45421 ctatataatg tatgtgtatg ataatcagcc agtattttca tttgttaatc ctctccaagt
    45481 attttgactt cttcttccaa cataacccca aacttggcat atacagcact ttttataaca
    45541 tatattaaat ccaaaatatc tttagcactt gcttctcctt gattgactac aaatccacaa
    45601 tgcttctcag atacttgtgc tcctcttaaa gtaagacctc tcaatccagt ttcttctatt
    45661 aatttacctg caaaatatcc tgttggtctc ttaaatgtac ttcctgcact agcaaaattt
    45721 aatggctgtt tagttattct tctcgtcgct aaatcatcca ttaaactttt tatttcatca
    45781 taatttcctt cttgtaattc taattctgct gataaagcta tatatccgtt tttcgatatt
    45841 atactttttc tatacccaaa ttccatctgt tcatttgata attcaaatat attgccttcc
    45901 atatccatca gtcttactga cttcactatg tccttcattt caccaccata tgctccagca
    45961 ttcatggcta atgctcctcc caatgtgcca ggtattcctg atgcaaactc aaaccctttt
    46021 aattcttgct tttggagagc tttccctaaa actgataata aagctcctga ctgagcagtc
    46081 atcctagtat catttatctc ataagaattg aaattatcag ctaattctat taccactcct
    46141 cttattccac catctttaat taaaatgttt gaaccatttc caattattaa atacggaata
    46201 gattcttttt ttaccaactt aagtacattt tttaattgtt cttcagttct aggtctaact
    46261 aatatatctg ctggtcctcc taccctaaat gaaatatgtt ttttcatagg ttcatcgact
    46321 tttatatcct ctttacttaa tatatttaat aaatttatat atatatcttg attattcatt
    46381 gtatttcctc ccaaattata ctgaacttta gctgtcatta ttatttaaat tacattagta
    46441 tatagtatta actatattca ataatatatg attttgctta tcatatagat tttatatttt
    46501 aagcttctta attacaaact atattcaata aatatataat aaagtaaaaa aaaaaaaata
    46561 acaacatata aaagaatata aataatttaa aaaaagcatc aatatcactg atatcaatgc
    46621 tttaaaaggc aatttaattc gtattattta tctaactatt tttgacatag caaagataat
    46681 aaatccctcc aactactatg gaacctccaa cgatatttcc aagtgttaca aataccaaat
    46741 tagttataaa ttgaaccata gttatatttg ctcctaatgc cattcccatt ggtataaaaa
    46801 acatgtttgc aacactatgt tggaatccac ataatacaaa tagcattatt actgtccaac
    46861 atgaagccaa ttttgacatt atatcttgag atgctgtagc catccataca gctaaaacga
    46921 ctagaatatt acaaagtatt gcttttaaaa acattgatgc tatagttgaa ttaaccttag
    46981 cttctgccac agctatagct tttgtagctg catctcctgt caatgtagaa gaataaaaca
    47041 caagtgcaac tagcaaaatt gaacctacta aatttgcaaa ataaaccagt ccccagtttc
    47101 ttaacatttt tcctaatgtt atttttttgt ccataagtgc aatagccatt aatgtgtttc
    47161 cagtgaataa ttctgctcct gccactacta caagcataag acctactgga aagaccatag
    47221 cacttaaaaa gctagctacc cctgggtcta tcttgccaaa agtttgacta actatcatac
    47281 tccccaatgc cccaaaacct ataaatgctc ctccaaataa acctaataga attgcgtttt
    47341 gtgtcgaaat atttgctttt ttaatgcctg cacttatagt tgcttgtgca gtttccccag
    47401 gtgtcaagaa tcctttactc ataaatcctt cccccttaaa aattatactt tcccttgtct
    47461 ttttatggca ataattatat aataacaata tgatatataa taaattttat taacataaaa
    47521 aatatactat atacttatat atacttgtct catataatta tactatttat tatataacaa
    47581 ctttgttaat ttcgaaataa tttatattga atttgaaaaa tttacctttt gattatttat
    47641 tttacttttt aattattgag gtattatata taatagatat atgtttgtgt tattatatat
    47701 aattacttat tttaataaat atttgaagga gggaatttta tgtttgatat atcatttttc
    47761 gaaatgtcaa tatatgaaat agcagttact acaatatata taattaattt catggttata
    47821 ctaaacctta tctttagaga aaaaagaaac ataaatacaa ctttaacatg gcttcttata
    47881 ttagtattga ttcctgctct tggattcata ctttatatag cttttggcag aaatatttca
    47941 aaaaacaata tgtttagatt gaaagaaaag gatgataaga taatcaaaag taatatacta
    48001 gatactcagg ttaaactcca atctacatct gaaattgatt cagatataca tcaacataag
    48061 gatatgattt atgcattggc taattcaaat aatgctcatt ataccaataa taacgatgtg
    48121 tggatttatg ctgaatctag tcaatttttt aacagtcttc ttgaagaact taaaaaagct
    48181 aaaaaatata taaacataca attttacatt ttcaaagatg acaaaatcgg aactgaaata
    48241 atagacattc ttatagacaa agctaaagaa ggtgttgaag taagactttt atttgatgca
    48301 gttggtggta gaacactaaa aaatagtaca ctttccagac ttaaagaaag tggtgttaaa
    48361 gttggaagct tcttcccatc attcttgaaa attgtaaact ttaacttaaa ctatagaaat
    48421 caccgaaaaa ttgttgtcat tgatggtaag gtaggatttg taggtggata taatgttggt
    48481 gatgaatatt taggtagaaa tccaaaattt ggtctatgga gagatacaca tactaagctt
    48541 actggtgatt gcgtaattga cctaaatatg agattcatcc ttgattggag atatacaact
    48601 aaagaagatt tagatttaga aaagtacttt atagaagaag atacaaattc taatcatcct
    48661 tctgaaaata ttgggattca gatagtatca agtggtccag atatatcaga acttgatgag
    48721 attaaatatg gttaccttaa aatgattcag aaagctagaa agtatatata catacagagt
    48781 ccttatttaa tacttgactc aacttttata gatactttaa aaatagcttg tttatctggt
    48841 gttgatgtga gagtgatgat tccttctaaa cctgaccatc cctttgtata ctgggcatca
    48901 tattcatatg ctggagaact tttaaagttt ggagctaaaa tctacactta tggtcaaaat
    48961 gcatttttac atgctaagac aatagttata gatgactcta tatgctctat aggaacagct
    49021 aatatggata tcagaagttt tgagcttaat ttcgaagtta atgcatttat gtactcttca
    49081 aaaaaagcat tggaacaaag aattatattt gaaaatgata ttttagattc taaggaaata
    49141 acattagata tatataattc taggtctact tatataaaaa tcaaggaatc aatttcaaga
    49201 ctattatcag cagttttata aatcttttat tttataaaaa ttttacattt tggtttattt
    49261 tttaaagttt tttatatgta ttattttttt tttattgaat ttagctcatt ttttttattt
    49321 tttttaacta ttctgaatta ttttatatta taatagacta gtatagtttt tatttttaat
    49381 aggtaacttt tttgcattgt ttttataaat aaagaaattg ttgtatatta ttctttcttt
    49441 attgttttgg ttttgttcac ccacataatg ttggggtcaa ctcatggtta attattttat
    49501 gaaaaataat aactttaaaa agaaaagggg gatttgttat gtgcgatttt atttcacaca
    49561 acaagcaatt atttgacgaa ctagatatat ttattgactc tcttgcaaca aaggaaggtg
    49621 cgttaataca agttctacat gaagcccaag gtatatttgg atatcttcca aaagaagttc
    49681 aacttcatgt tgctagaaaa cttggagtag ctcctgcaaa ggtatatggg gttgttacat
    49741 tctattctta ttttactaca gaacctgtag gtaagtataa aatcagtgtc tgtctaggaa
    49801 ctgtatgttt tgtaaaaggt gctgacaaaa tcttaagtgc ttttgaaaaa cagcttggca
    49861 taaaagttgg agaaactact agtgatttta aattttcttt agaaggttta agatgtttgg
    49921 gggcatgtgg tttagctcca gttgttactg taaatggtaa agtttatggt aaagtgaaac
    49981 ctgaccaagt aagcgaaata ttagatactt atagagaatt agaattgaat tgttaagagg
    50041 ggtgaataaa taatgcgtaa agtaaattca tttgatgaat taaaagtttt agctgatgag
    50101 ctgaaaccta atcttagctt aagaaaaaat tattctaagg aaaatgtatt aagaagagaa
    50161 ttacttgttt gttgtgatac tggatgtaca tcttcaaaca gtctagaaat agttagtgaa
    50221 ttagaaaatg aaataaagaa atcaggaatc caagataaag ttagtgttcg tttgacaggt
    50281 tgttttggtt tctgtgctca aggtcctatt gtaaaagttt atcctgataa cgttttctac
    50341 gtaaaagttg agccatctga tgctgaaaag atagttcaaa gccacttaat tagaaataca
    50401 gttgtcgagg aattattata tgaggaagaa tctttaagta aaaaagttga agaacaagaa
    50461 gaaatgtctt tctacaaaaa acagttacgt atagctttaa aaaattgtgg tcttatagac
    50521 ccagatagta tagaagaata tattgccaat gatggttatt tagctctagg caaatgttta
    50581 acttctctta ctcctcaaga agttattgca gaggtaaaaa cttctggcct tcgtggtaga
    50641 ggtggtgcag gtttccctac tggctctaaa tgggaagctg cttcaaaaaa tccagctggt
    50701 ccaacagata aaaagtttgt tgtatgtaat gctgatgaag gtgacccagg tgcatttatg
    50761 gatagatctg tacttgaagg tgaccctcat agtgtactag aagctatggc aatatgtgga
    50821 tacgctatag gttctgatac aggatacata tacataagag cagaatatcc aaaatctata
    50881 gaaagattaa aagtagccat agctcaagct gaaaaatacg gtttacttgg cgaaaatatc
    50941 ttaggcactg gatttaactt taaattagaa ttaaaatatg gtgcaggtgc tttcgtttgt
    51001 ggtgaaggta cagcgctaat gcattcaatt gaaggtagac gtggtgaacc tagaatgaaa
    51061 acttatagtt cttctaagag tggtctatgg aaatctccta cttgcttaaa taacgttgag
    51121 acttttgcta acatacctgc aattatactt aagggtggag attggtttgc caacttaggt
    51181 actgaagatt ctagtggtac taaagtcttt gctcttggtg gaaaggtaga aaatgtagga
    51241 cttgttgaag ttccaatggg tactacttta agagatattg tatttgaaat tggtggggga
    51301 atccctgaag gtaagaaatt taaggctgtt caaacaggtg gaccatctgg tggatgtata
    51361 ccaactgaac atttagacac tcctatagac tttggttcat taagttctat cggttcaatg
    51421 atgggctctg gtggtatgct tgttcttgat gaatctgact gtatggtaga tatagccaag
    51481 ttcttccttg agtttactgt tgaagaatct tgtggtaaat gtacaccttg tcgtataggt
    51541 actacaagat tattagaaat attaactaaa attacagaag gtaatggtac tctacaagat
    51601 ttagatgacc tagaaaacct tgctgaaact atacaaactg cttcattatg tggtcttggt
    51661 aaagctgctc caaaccctgt tttaagtact cttcaatatt ttaaagatga gtatatagct
    51721 catgttgtag ataagaaatg tcctgctggt aaatgtcaga acttactttc ttactttata
    51781 acagatgact gtaagggttg tacaaaatgt tctagagtgt gtcctgctgg ttgtataaca
    51841 ggttctgtta aggaacaaca tacaatagat acttctaagt gtctaaaatg tggtgcttgt
    51901 atagataatt gtacatttaa cgctataatc aagaaataac ttatttggag gaggaataac
    51961 aatgagttta gttaatttaa ctattaatgg caaacacgtt tcagcaccta gtggaacttc
    52021 tatcttagat gctgcgaaat taataaatat aaaaatacac aacctttgtc atttacatat
    52081 gaatgaaata gataagcttg acacttgtgc ttcttgtcgt gtttgtatgg ttgagacaga
    52141 gagaggatta gttccagctt gtggaacagt tataaaggaa ggtatgaaag ttcagactaa
    52201 tagtgctaaa gcattaaatg ctcgtagaac tattgttgaa ttattgttat cagaccatcc
    52261 acaagattgt tttgtctgtg aaaaaaatgg agattgtgaa ttacagacta tagctgctga
    52321 tttaggagta agaaaaataa gatatcaagg ttctaaatct tttgcaggta aagatacttc
    52381 tactaaatct ttagttaaag accattctaa gtgtatatta tgtagaagat gcgagactgt
    52441 atgtaatgac atacaaacag taggagcatt atctggagtt aatcgtggat ttaatacttt
    52501 agttagtaca ttctttaact ctgatatggt tgaaacagag tgtacattct gtggtcaatg
    52561 tatatctgtc tgtccaactg gagcattaac agaagtagat aatgtaccta aactttggga
    52621 tgttttaaat aaaaaagaaa agactatagt agtgcaagtt gctcctgctg taagagttgc
    52681 tattggtgaa gagtttggat tagaaccagg gtcaatctct acaggaaaaa tggtagctgc
    52741 tttaaaagca cttggatttg aacatgtatt tgatacaaac tttggtgcag attttactat
    52801 aatggaagaa gctactgaat ttatagaaag aatccaaaaa ggtgaaaatc ttcctatatt
    52861 aacaagttgt tgtcctgcat gggtaaactt cttagagcac aattatccag acaagttaaa
    52921 cttagcatct agctgtaagt ctcctcaagg tatgtttggt tctatagcta aaaactacta
    52981 tgcacctaaa atattaggaa tcaatcctga agaactttat gttgtatctg taatgccttg
    53041 tgtagctaaa aaatatgaag cttcaagaga agaattaagt gaatctggaa tactagatgt
    53101 tgacctttct ataactacta gagagttagc taagatgata aaagaagctg ctatcgattt
    53161 acctaatctt caagaccaag attttgataa tcctctagga aaatctacag gtgctgcatc
    53221 tatatttgga gcaagtggtg gagttttaga agctgcccta cgtacttcat atgagaagat
    53281 aactaataag actttagata atgtaaactt cactaatgtt agaggtctta aaggaattag
    53341 ggaagcaagt atagatgttg atggaactac agttaatgtg tgtattgtaa atacacttaa
    53401 aaacgctaga aagattatgg ataaggtcag aagtggagaa tgtaaatacc atatcataga
    53461 agttatggca tgtcctggtg gatgtgttgg aggtgctggt caaccttatc accatggtaa
    53521 tactgaaata gttgatagac gtgcaaatgc gttatatgaa atagatagaa ataaggctat
    53581 tcgtaaatct catgagaatc ctgatttaca agctatttat aaagacttct ttggagaacc
    53641 aaatagtgat gtagctcata aataccttca tactcattac tttgataaga gttgtgtcta
    53701 tggagagtgt ccacaagaat gtgcttgtga agaagctaag tagtcataac aaagtataaa
    53761 atttattctt aaaaaaggat tgtcttaaaa tacttttacg taatttaaga caatccttat
    53821 taatttcaca aattaaatta tatttctaaa tagacttatt ctttaattaa cctatctatt
    53881 aattaaagaa tacctgccat tctcataagt tgttttgcat ttgtagtttt acatcttcca
    53941 tcttttatct tatagtcaaa atgaatttta tcgtctacat agtactctga gaaatgatag
    54001 tttacaactt caatatctcc actacctgaa aggtcacata gctcaaagtc atgtgttgtc
    54061 accatactta taacccaggg ttttgataat ttctttactg cctcacttgc tccaataatt
    54121 ctatcagcag agttagttcc cttaaaaatt tcatctacta gaaccaacat tggcagttct
    54181 tttaaactat attgaatcat tgattttatt ctaagaatct cagcatagaa agttgatatt
    54241 ccttgactta catcatcttg tattctcata gaagtaaata tagccataca ggtcgcatca
    54301 aaactcttag cgcatactgg agcacctgca tatgccagca ctaagtttac accgatactt
    54361 cttaaaaatg ttgtctttcc agacatattt gaacctgtga taatacaagt ctgagaattt
    54421 aattgtatcg aatttgctat gacacttttt tctgctatta atgggtggta tacctcttca
    54481 actttcaatc ttggagtatc ttcatatttt attactggaa aacatgtatt ttcctttaca
    54541 tgagaaacaa ctgttaaact tattagagct tcaaattctc caatagattc aaaccaatct
    54601 ctaatttgat ttccatatat acctttccat cgctccaaag cttctgcaca atgatagttc
    54661 cacatcaaaa caccacaggc aattatatat agtatctgat tataccttag gtttacagca
    54721 tttcctattg aatttaattg tttaacacct cttgaaactc cacttccctt agaaagttta
    54781 tcttgtaatt ctttcaaata tgcactctca aacgtctctt tttctaaaac ctcaaacatt
    54841 ttttcatata cttgtatact tctactaaat gaaaataatg gagcaagagt ttgcataagt
    54901 cttggatatc caaaaccact aaaccctaac tggattataa aaagtaaaaa aacaggtaaa
    54961 acaggtaaaa atcctaacat acaaaatata aaagataaga ttgttgcagt tggtaaaccc
    55021 caagtaaaaa tatgcatcca ttttggtata tacacattct tattttctcc atactctata
    55081 aacgattcta tactactatc tgaattatct ttttgctctt taccaattat attactgagt
    55141 gtttgaatat gaatagaaaa atcatctttt gataaaagtt cttttatagc tctctgtctt
    55201 tctaagatta cattctcatc tggattttca ttccacaaat atttggctaa aaaatttttc
    55261 ccataagatg tattagctac acagatatat tgatacaatg atgctcttcc aaataagtct
    55321 aaatcttttg cttgcgaatt ttcatctttt aagtattctt taccatctat tttaaattcc
    55381 ttccaaccat ctccaaatct cttaatatac ttatttaata cttcgctctt acttttaaaa
    55441 tatgtttctt cttcttttac tttactatga ataactaaga gtgctataaa tattattatt
    55501 gataaaacgc caacaaaaat acctactata tttttattgt aaaatccaat agcaagccct
    55561 gctatcacta tcaaaaaaga tattaatctt gctgtactta tgttatttga ctttttactt
    55621 aagagcaatt ctttttcagt actactttct aaaagtttac taaaatattc tattcccata
    55681 aaaaattcca actttcttgt tatataatga aactcaaata taaaattaat ctagtattat
    55741 tataatctaa cactgtactt taaattatcc ttatataata tatcttatta ataaacatta
    55801 tatatataca acttgatttt aatttataag gtgtaggaca taatcctaat caatatttaa
    55861 ttaaatgatg tgtagttcac tattattcag tactacttag tatcgattaa ttttgataat
    55921 tttttagtaa attcttgagc tgcatcgtat cccattcctc tttgtctaaa attcattgct
    55981 gctacttcta atatcatagc agtatttctt ccaggtttaa caggtacaac taatttctct
    56041 acattaacac caagtatttc ttcatattct ttatccagtc ctagtctatc ataatactta
    56101 gtttcatccc aagcttctaa atgaactact aaatctatca ttttagaatt tttgattgcc
    56161 cctactccat ataaacttct aacatctatt attcctattc ctcttatctc aagaaaatgt
    56221 cttattagtt caggtgactg acccacaagc atactttcat ctaattttct tatttctaca
    56281 gcatcatctg caactaatct attacccctt tgaatcaatt ctaatgctgt ttccgacttt
    56341 cctatactac tttcaccttt tatcagtaca cctataccat aaatatctac tagcaccccg
    56401 tgaattgttg tatgtggtgc taacttatta tccaaatagt ttgacagtct atttataagt
    56461 ctagtggtat ttctatttgt gctcaaaagt attcttttat attttttagc cttttcaatt
    56521 acatctggct tgatttccaa gtctctagaa attattaacg ctggtatttc atatgagaaa
    56581 aatctatcta gtacttcttc tcgaatatga ctatccatta atccaaaaaa attatattct
    56641 gtctttccaa ctatttgtat tctttcatat gcaaaataat caaaatatcc tgcaaactgt
    56701 aatcctggtc tatttatatc ttgagagtag acataataat ctacatcatc aggcatgtat
    56761 actactttta acttcaactc ttttattatc tctcttattg atactttatt gctagtttcc
    56821 attaattttc ttcctttcta aaaaaattat aaacattatc tgcaatgctt ttattcattc
    56881 catccacttc taaaagttct tccatagtag ctttttttat agcatctaca tctttaaagt
    56941 gatttaataa tgatttcttt cttttttcac ctattccttg tatatcatct aagatagatt
    57001 ttgtcaaagc tttatttctt agacttctat gatatgttat tgcataatta tgaacttctt
    57061 cttgtatact agcaataaat ctatataaat tagttgtttt atctagttct atttcttttt
    57121 ctttgcatat caatccttta gttctatgct tatcatcttt gtacattccc caaagaggta
    57181 tatctacatc attgagttct agtacttttt ttactgcact aacttgtcct tttccaccat
    57241 ccaacaatat caagtcaggt aaatttccat gttttaatct tctatctaca atttcagcca
    57301 tagaatcata atcattaggt ccaataactg tctttatttt atatctccta tattccttct
    57361 tatctttttt agcattcgta tacactacca tagaaccaat cgaatctaca ccttgtatat
    57421 ttgatatatc ataagcctct attcttattg gcaacttttc tagatttaat atttgcttta
    57481 attcttccaa tgctccaata cttttttcat attttctttt attcatatca gaaaattttt
    57541 caagatattc tacagcattt ttcctaacca tttcaactaa gcttttcttc tctccctttt
    57601 gaggaactct tattgttact ttttgcccct ttttagatga cagccattct tctaatatag
    57661 aactatcttc tatctcatct tctataatta attctttagg tatatactct tgttcattat
    57721 aaaattgttt tacaaatgaa cttaatatag aagctcttgg actatccatt accccttcta
    57781 atataaaatg ttccctacct acaatcttac catttcttat aaaaaatacc tggacacaag
    57841 cttcattatg agctcttgcc attgcaacca catcttggtt taagtcactt acagtagcat
    57901 ctattttttg tttctgtatc atctcttcaa ggctctttat cttatcccta tatacagcag
    57961 cctcttcaaa tctaaagttc atagaactct cattcatttt ttcctttaaa agttccatca
    58021 atttttcttc tttaccagat aaacacatta taatctcttc tatcattttt ccatactctt
    58081 ctttagaaac atttcctgta caaggtccta aacatttatt tatgtgtaga tttaaacaag
    58141 gtcttgtctt attctttatt gctttatcta tatctatttt acagcttctt attggatacg
    58201 tactacttat aagctccaag gtatcattca cagcagttat attggtatag ggcccaaaat
    58261 actttgcctt atctttaaga actcttctaa cttttaagat tcttggataa tcttcattcg
    58321 tagttacttt tatatatgga taagttttat catctctaag cactacatta tactttggtc
    58381 tgtatctttt tattaaatta cattcaagta ttaatgcctc taactctgaa tccgttataa
    58441 tatactcaaa cttatatata ttcttaacca tagattttac tttagatgta tggttttttg
    58501 aagattgaaa atactgcctt accctatttt ttagagatat ggcttttccc acatatatta
    58561 tatggtcgta tttgtctttc attaaatata ctccgggctc agaaggtaat ttttttaaat
    58621 gctcttgtat atcaaacatc tcgccctcca aaacattaaa ttttatacta aaattatacc
    58681 ctaaaatatg aattttaaaa agttatacaa tttccagtgc tttaaacaag ttaaagccca
    58741 tattcccttc tataattgat ttgaaaacaa ttttaaccat gaagagtaat atggacttta
    58801 ctttgtatta ctatttacaa cttatatttg actaatttac ttataacaaa acagtttatc
    58861 gtatatctgc tctaaaattt ttcacaattt atataactta agaaaaatat ttctttaaga
    58921 attgacctgt ataagaacca tcaactttag atacttcttt tggtgttcca gttgcaacta
    58981 tagttccacc cttatctccg ccttctggtc ctaagtctat tacataatca caagtcttta
    59041 taacatctaa attatgttct atgactataa ttgtatttcc tgtatctgct agtctttgta
    59101 atacttggat tagcttgtct acatctgcca tatgaagtcc agtagtaggt tcatctaata
    59161 tatacaaagt ctttcctgtt ggtcttttac taagttcagc agctaatttt atacgttgtg
    59221 cttctcctcc tgataattga gttgaaggct gtcctaattt aatatatgaa agtcctacat
    59281 ccattaaagt ttctaatttt ctttttatat ttggtatatt ttcgaaaaac tcaacagctt
    59341 cttccacatt catatctaat atatcagata tagttttatc tttgtatttt acttgcaatg
    59401 tttctctatt gtatctttca cctttacaaa tttcacaagg aacatataca tctggtaaga
    59461 aatgcatttc tatttttatt attccatcac ccttacaagc ttcacatcta ccacctttta
    59521 cattaaaact aaatctacct tttttataac ctcttgcttt agcttcatta gtagatgcaa
    59581 aaatatctct tataaaatca aatacacctg tataagtagc aggatttgaa cgaggtgttc
    59641 ttccaatagg actttggtct atatttataa ctttatctat actttcaatt ccttttatct
    59701 ccttatgctt accagctctt tgcttaagtc tattcacttt acttgcaaca cctttgtaaa
    59761 gtatatcgtt tattaatgta ctttttccag aacctgaaac acctgttatg catgtaaact
    59821 tacctaatgg gaactttaca tcaatatttt tcagattatt ttctgatgct ttaactattt
    59881 ctataaaact tccatttcct tctctagtag tctcaggaag caatatttgt tttttaccac
    59941 ttaaatattg acccgttatt gagttctcat tttcaattat ttcatctaaa gtaccctgtg
    60001 caactatttc accaccatgt atacctgcac ctggaccaat gtctataaca taatcagctt
    60061 ctctcattgt gtcttcatca tgctctacaa cgattaaggt atttcctaaa tctgttagat
    60121 gtctaagagt agttataagc ctatcattat ctctttggtg aagccctata cttggctcat
    60181 caagtacata taaaactcca accaatgcag aacctatttg agtagctaat cttatacgtt
    60241 gcgcttctcc tcctgataaa gttccagctt ttcttgataa atttaaataa tcaagaccta
    60301 catctcttaa gaacatagct cttcctttta tctcttttat aatctcatga gctataaatc
    60361 tctgtttttc tgtaagattt atattttcca taaagtcaat caattcattt acagataagt
    60421 ttgtaacttc cattatattt tttccatcaa ctaaaacaga taaaacttcc tttttaagtc
    60481 tcatcccttt acactttgga catggtctct ctgccatgta ttcttcaatc ttatctctca
    60541 tgtaatctga attagtttct ctatatcttc tctctaagtt tactattaca ccttcaaagt
    60601 atgatttata atttctagtt cctccaaatt ttgactcata agtaaactct acaattatat
    60661 tatctttacc ataaagtaat tcctccacaa aatcatctgg taaatcttta aacggtgttg
    60721 tcaaagatac attaaaatgc tctgctaaac tttttatcat tttactgtaa taagtgtcat
    60781 cattaacacc tgttgaaccc catgctgcca ctgcaccttg ttttattgat aaatctttgt
    60841 ttggtactac taattctggg tcaacttctt tactttcccc aagaccatta caaacatcac
    60901 aagcaccaaa gggagcatta aatgaaaaca ttcttggaga taattcttct atgccaattc
    60961 catgttctgg gcatgcaaat ttagttgaaa aaagaatttc ttctccatct attatttggg
    61021 ctattacaag accatcagat agcttaactg cagtttcaat tgaatctgct agtcttgatt
    61081 ctattccatc tttaattact attctgtcca ctacaacttc tatattatgt ttttgatttt
    61141 tatttaagat tatatcatca ctaacttcat agttttcacc attaactcta actcttacaa
    61201 atccttcttt tttgatatta tcaaagactt ttttatgtgt acctttttgt cctctaacaa
    61261 ttggtgaaag aatttgtaat ttcgttctat ctggaaaatc tagcatttta tcaactattt
    61321 cttgaatagt catctgactt attttttcac cacatgtagg gcaatacaca tctcctactc
    61381 ttgcaaataa tagtctcaaa taatcatata tttctgttac tgttccaacc gtagaacgtg
    61441 ggttttttga ggtagtcttc tggtcaattg agatagctgg tgataaaccc tctatatatt
    61501 ctacatttgg cttttccatc tgacctaaaa attgtcttgc ataagcagat agactttcta
    61561 catatcttct ctgtccttct gcatatattg tatcaaatgc caaagaagac ttcccagacc
    61621 ctgacaaacc tgtaaaaaca ataaatttat ctcttggtaa ttctaaatct atatttttta
    61681 aattatgttc ttttgctcct cttattataa ttttatcttc cataatgttg tttttacctt
    61741 tctatttttc caatttttct tctaattgtt tcactttatc tctaagttct gctgctcttt
    61801 caaattgtaa gttttgagca gcttccatca tctctgattt taatttatct atattagctc
    61861 ttatttcgtc tttatcatct gtatcagata ttccatatac aacttcttcc tctgcaacct
    61921 tagtagcttc tatactatct cttatatttt tctgtatagt tttaggaatg atattgtgct
    61981 cttcattata taaattttgt atatctcttc ttctctttgt ttcatcaata gcattttgca
    62041 ttgagtcagt tatcctgtct gcatacataa ttactcttcc atttttattt ctagctgcac
    62101 gtccaatagt ctgtattaaa gctgtttcgg aacgtaagaa accttctttg tctgcatcta
    62161 aaattgctat aagtgaaact tctggtatat ccaatccttc tctaagtaag tttattccta
    62221 caaggacatc aaacttccct aatcttaaat ctcttattat ttctgttctt tctaatgtaa
    62281 ctatatctga gtgtaaatat ttaactttta tccctatttc tttaagatag ttagtcaagt
    62341 cctcactcat ttttttagtt aaagtggtta taagcactct ttcatttttt tctatagttt
    62401 tagttatttc tcctaccaaa tcatctattt gattatttat aggtcttact tctacaattg
    62461 ggtctaaaag gccagttggt cttattatct gttctgctat tgtctctgag tgttgaattt
    62521 cataaggccc tggtgtagca cttacaaaca acatttgatt tatatttcct tcaaattctt
    62581 gaaaatttaa tggtctattg tcaagtgcag aaggtaatct aaaaccattc tctataagtg
    62641 atgtttttct agacctgtct cctgcataca tacctcttac ttgtggtata gtgacatgtg
    62701 cctcatctac aattatcaaa taatcatctg ggaagaaatc cattaaagta tatggtcttt
    62761 ctccttctga ccttcctgta atatgtctag aatagttctc tatcccctga caaaatccta
    62821 tttcctttaa catttctata tcatacttag ttctttgttc aattctttgt gcttctaata
    62881 gcctatcatt ttccttgaaa aatttaattt gttcttgaag ctcgttttct atttctacaa
    62941 ttgccttttc aattctttct ggcgttgtta catagtgaga tgctgggaat ataacaacat
    63001 gatttctagt accaacaatc ttccctgtta cataatcaat ttctgttatt ctatctacct
    63061 catcaccaaa aaattctatt cttattgctt tttcatcatt actagctggg aatatttcta
    63121 gtatatctcc tctaactcta aaggtacctc gtgtaaagtt tatgtcattt ctttcgtatt
    63181 gtatctctat taaccttttt attacatcat ctctgtctct ttgcatacct ggtcttatag
    63241 ataacataag ttctttatag tcttttgggt caccaagtcc atatatacaa gatacagatg
    63301 atattattat agtatctctt ctctctaaaa tagatgctgt agcagaatgt cgaagtttat
    63361 ctatttcatc atttatgctt gcatcttttt ctatataagt gtcactgtga gctacatatg
    63421 cttctggttg ataataatca tagtaactta caaagtattc aactgcattg tctgggaaaa
    63481 attctttaaa ctcactgtaa agttgtgctg ctagagtttt attatgagcc attattaggg
    63541 taggtttctt aacttgttga atgatatttg ccattgtgaa tgtttttcct gaacccgtta
    63601 cacctaataa tgtcgaaaat ttctcatttc tatttataga atccactatt gatttaatag
    63661 cttctggttg gtcacctgta ggtttgaagt ctgattttat tttaaaatcc atagtatcac
    63721 tccttttttc gaacattagt tcctgacatg tattattata tcataccttt ataaaattgt
    63781 ttaagttatt ttaaatattt tacccattta aattttactt aaatctaaaa cataaaaaaa
    63841 gacttctatc tttgcaatag aagtctataa ttcatggaaa ataaatactt tattcaactt
    63901 acataataaa atcaaatgct tctttttcaa ctccatctaa ttttaaatta aacattttta
    63961 atatagttgc tgcatgtgct ctcaggtctc cttcttctat aaccacacca cctttaatat
    64021 ttggtccaaa agctataaat ggaggtctat tacctttagt aggtaaatga ccatgtgtcg
    64081 caacagcaaa cttgtaatct tttatatctg aatcttttat aatacttcct tcgaaatcat
    64141 ttccaaatga aattccttca agaccttcaa ttaccatttc aaaatcacct tcaagaccaa
    64201 ggttagatgc ttcctctttg gtaaaaatat tctctatgaa taattcttct ttaaattctt
    64261 caagcagttc tcttaattct gttaacctat ctaaattatt tactattatt tgagctgata
    64321 ttccagaaga atttacatag gcatcaaaat ccttaacttc tccattttca acttttataa
    64381 atccatttga tttaagcaag acatttggat ttataacttt atcaactcta agatgaccat
    64441 ggtccccaag tattataaaa tttgtatctt tgtatgtacc tgcatccttt gtagcctgaa
    64501 taatatctcc taaccattta tcattcattt ttaatgcttt atctacttcg tcattaaata
    64561 agccataatt gtgtcgtgta tggtccaatg tagctaaatg tattagcatt aaatctggtt
    64621 tatattcctt tattatatct actgaacagt ttactccaaa taaatccatg ttgggttcta
    64681 atttccaatc gatataatga caatgtctat tgtaaatacc gtccattata accttactgg
    64741 aacttcgttc aaaagtttct cttgggtcat cttctctact aggagcccat atttctgcaa
    64801 tattataatc tatatttgga tttgctccca ttacaggcca taatacacta gaggtagtta
    64861 gattattttc ttttgctaca tctataatag tttttgcttt tatgtccttt gaataccagt
    64921 accagtcttt attttcttta tttgggtcaa atttttcatt atgatatata ccatgttttt
    64981 taggatatac tcctgtaacc atactagtat gacacggata tgtcagagtg ggataaacac
    65041 aatttatatt ttttactatg gagcaatttt ctagtataga tttaaagttt ggtagatttt
    65101 ttacaaattc caagtctttt tcaaataaag aatctactga taatactata acctttgtca
    65161 tttacattac ctcttcattt atatattatt gaatactttt aatcaactta caagtctata
    65221 taattattta tttgttttat ctattactgc ttgcattgca gaagttcctt tgcttactac
    65281 atcttttgga tttgcattta aatcagtaag ccccttttta atctcatcca ttagcttatc
    65341 agttgcttcc ttgtatccat ttaacattgg aagtgcaaca gcatattgta attggtcagt
    65401 tgcaacctta tattgaggtt tttctttata taatgtttgt aacttttctg agtttgttgc
    65461 atcttcagtt gttggtaagt atcctgttga tgaactaaac tgaacgattt tatctgtttg
    65521 tgtcatccat tctataaatt ctgcacttgc ttctttttct ttatctgttc caccttcaag
    65581 cataactaga ttagctccac ctgtaggagt tgcgtatttt tgattttttg gtaagaatgc
    65641 tgttgatatt tcaaacttac cttctgtttg ttgcataagt cctgctaaat ctgctgttga
    65701 tgtaaatatc atacctactt taccatttac aaaatcttgt tttgctacat cccaggcatt
    65761 ataatcttct ccaggaggca ttttcattat tccagctttc atcatatctt gccagaactt
    65821 aactggtgct tgtcctgcct cattattaaa tgctactttt ttaccattag ttatttctcc
    65881 tccactttgc attaccatag cttcatagaa ccaaatatct attggcgttt caaaaccata
    65941 agtatcttct gatttatttg tcattttact agcataatcc ttaagctctt cccatgattt
    66001 tggacctgaa gggtcaagac ctgctttagt tgccatatct ttatttatgt ataatattgg
    66061 tgtacttcta ttaaatggca taccaacaaa cttatcctca taatatgaat ttgttaaaag
    66121 accagaaaag aatctttctt gatatgtttt atcaaaatat ttactcatat ctactaaagc
    66181 acctgctgat gcgaatgtac ctgtttgagt tatttctaat aatgttacat ctggttggtc
    66241 tttagatgtt aaggctgcct gtagttttgt tgcattttca tagtaatctt tctgttttaa
    66301 catatttact tgtacatcct tatgttcact attaaattgg tcaaccattt tttgagcagt
    66361 ttctccatta gttccaccta aaggagccca gaaatctatc ttcgtaatat tgctatcagt
    66421 cttttctcct ttagatgcat ttgcatcatt agaatttgaa cacccaacta aagaacctaa
    66481 taataatgtt attgctaatc ctgttgctaa ttttctttta agcttcattt tcatcctcca
    66541 aatttagtct ttaatttttt attttattcc tgaatacata aaagcttctt ttactttttt
    66601 attcgcaaat atataaacaa gaattattgg taaaattaga attatatttc ctgccattat
    66661 tacattccat gcagtaattc cttctgaaga tttaaggagc gcaactccta ttggtaaagt
    66721 tcttattgca tctgtatttg tcattactaa gggccagaaa taattattcc aatggtatat
    66781 aaaattaaat aatataaatg ttattactgc tggttttgcc ataggaacca ttattctaaa
    66841 cattattttt aagtcagaag cattatctag tttagctgct tcaataattt catctgaaat
    66901 ctgcataaaa ttctgtctaa gcagaaatat cccaaatgaa gatgtcataa atggaattat
    66961 aagtggaaca taagtattta taaatccaaa cttactcatc atcatataga ctggtagaaa
    67021 agttacttgt ggtggaatca taagtcctac aagcaaaagc ccaaatagca ttttttcacc
    67081 tttaaattta atcttagaaa atgcatatgc tgctggaacc ataactaaaa attgtacaat
    67141 taaaacactt cctgttacaa atacactgtt tagcaaatat ctaaaaaatg gacctgaatg
    67201 ccatgctgta ctataatttt ctaacactat atcatttggt ataatagtgg gcggtaattg
    67261 catcatttct tgtggatttt taaaggatgt agatagcatc catataaaag ggaatataaa
    67321 tattgcagat aaaagtaata gaattaatat ttctattgat tttgatgcta ttttttttat
    67381 atcaactttg tcttttttga taggttgtat aatatccatt gtctcctcct tttggataaa
    67441 ccttataata tctacttttt ctttattgat aatgaacttt ctttgataag agtctaaagt
    67501 tagcatatgt agcaatcata actatagcta acaaaaacat actcgctgca ctcgcttgtc
    67561 ctatgtcata gtatataaat ccagactcat atatataata agataacatg ttagttgaac
    67621 ccactggccc accttgtgtc attatactta tctcatcaaa tacttgaaaa gatgcagttg
    67681 ttgttgttat cataagaaaa aatattgttg gtgataatag tggcaatgtc actttaaaga
    67741 aattcgttat tttgtttgca ttatctaatt tagatgcttc atatatatag tttggtatgg
    67801 atttaagacc tgataacact attaagatat tatatccaag agtcttccat attcctatta
    67861 gaattagtga cattaaagct gaactttcac ttgaaagcca ctttgatgtt ggtaaatgta
    67921 atgtcgatag tatccagttt aaaaagccat attgtggctc cataatccaa agccacaata
    67981 cagctacaga tactagtgaa attacatgtg gtgtaaagat tatactttgc gttaaattgt
    68041 gtattataga atttttattt agccaaacag caagtactat ggctagtgat atagatatag
    68101 tcaccataaa aaaagtataa attgcagtgt tcgttatagt ttgtataaac tctggattct
    68161 ttaaaagttc tgtataattg tttaagccta cattttcaaa ggttgaacta agaagactac
    68221 cagatttgaa gcttagagat atcgtcttaa ttattggata gactataaac aataaagttc
    68281 ctataatagc aggtgttata tataaatatg ctttattaaa ttgctttttc ataaatatct
    68341 cctttaattt tagaatacat acttttacat acttttacat cttctattct ttctccattt
    68401 ttatcaaaat aatataaatc tttttctaat acacttattc ttaaagttct tccaatatca
    68461 aatatatcat tttccgtttt gactcttatc tctctattaa gaacagataa acaatatata
    68521 gtttcatttc ccaacacttc tttagttaca acttttccaa atagactaaa tacatcatca
    68581 tttttcccat ctatgtttat actctctagt gttgcttttt ctggtctgaa tccaaattta
    68641 atatcatttt ttatatctag tatgttcata caaggactcc ctataaattg agctacaaat
    68701 aaatttttag gattttgata tatcttcttt ggagaaccta tttgcattat ctttcctttg
    68761 ttcattataa ctatcttatc tgccatcgac attgcctcaa cctggtcatg agttacataa
    68821 acaaatgtag actttagttt ttgatgtaat tgtattaatt ctgttctcat ttgatttctt
    68881 aatttagcat ctaaatttga caatggttca tccattaaaa atacctttgg atttttaacc
    68941 atagctctag caagtgccac tctttgtctt tgacctccag ataagttgcc tggtttcaca
    69001 tttaaatatt ctctcaatcc tactacatca ataacttctg ttataagttt ttctcttttt
    69061 tctttttcta cttttttgtt tttaagacca aattctatgt tttctctaac agtcatatgt
    69121 ggatataatg cataattttg aaataccata gctatatccc ttttactagc atccgttttt
    69181 gtaacatcat tttcttcaat cttcaaccta cctgaagtta tatcttcaag acctgcaatc
    69241 attcttaacg tagtagattt accacatcca cttgggccta ccaatactgt aaaagaacca
    69301 tcttctatag taagatttaa attttctata actgtatttt ttttattata agattttgtt
    69361 acattttcta gtcttatatc tgccattttt atcctcccaa aatatttaat ttaattctac
    69421 gatttactta tagattaata atactcttaa tttgtaaagg atatattaag acaaagtgaa
    69481 tatatggtaa acttattaaa ataaaggtta aattataaac attttttgtt aaatatactg
    69541 aggttaagat gatatacagc ttatgttagc tataattttc tatatttttc atattcatgg
    69601 tttgctatca caatgtaatt ttaatttatg aagagtcata taaaactatt tattttttat
    69661 ataatttgat ttgaaagtaa aaaagacctc tatgggggat agaggtcttt aagtggggga
    69721 gattgagtaa tttatttttg ctatattaag ttttacaatt atattatgtc ccaaatttac
    69781 aaggtttaaa cataaaaaat aaaacttttt taatttttat aaatttacc
//
LOCUS       DAELJO010000003        67129 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELJO010000003 DAELJO010000000
VERSION     DAELJO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.003"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(364..1086)
                     /locus_tag="KPB88_000143"
     CDS             complement(364..1086)
                     /locus_tag="KPB88_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0052458.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1145..1822)
                     /locus_tag="KPB88_000144"
     CDS             complement(1145..1822)
                     /locus_tag="KPB88_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0052459.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1850..2665)
                     /locus_tag="KPB88_000145"
     CDS             complement(1850..2665)
                     /locus_tag="KPB88_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0052460.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2891..3101)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3484..5550)
                     /locus_tag="KPB88_000146"
     CDS             complement(3484..5550)
                     /locus_tag="KPB88_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0052461.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDENDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5627..8656)
                     /locus_tag="KPB88_000147"
     CDS             complement(5627..8656)
                     /locus_tag="KPB88_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0052462.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8709..10169)
                     /locus_tag="KPB88_000148"
     CDS             complement(8709..10169)
                     /locus_tag="KPB88_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0052463.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10261..10635)
                     /locus_tag="KPB88_000149"
     CDS             complement(10261..10635)
                     /locus_tag="KPB88_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052464.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(10811..11551)
                     /locus_tag="KPB88_000150"
     CDS             complement(10811..11551)
                     /locus_tag="KPB88_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0052465.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEAGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11572..12360)
                     /locus_tag="KPB88_000151"
     CDS             complement(11572..12360)
                     /locus_tag="KPB88_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG0052466.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12448..12538)
                     /locus_tag="KPB88_000152"
     tRNA            complement(12448..12538)
                     /locus_tag="KPB88_000152"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12502..12504),aa:Ser,seq:gct)
     gene            12797..13561
                     /locus_tag="KPB88_000153"
     CDS             12797..13561
                     /locus_tag="KPB88_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0052467.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(13842..14957)
                     /locus_tag="KPB88_000154"
     CDS             complement(13842..14957)
                     /locus_tag="KPB88_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0052468.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(14998..16524)
                     /gene="glpK"
                     /locus_tag="KPB88_000155"
     CDS             complement(14998..16524)
                     /gene="glpK"
                     /locus_tag="KPB88_000155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0052469.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            16817..17914
                     /locus_tag="KPB88_000156"
     CDS             16817..17914
                     /locus_tag="KPB88_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0052470.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(18330..18842)
                     /locus_tag="KPB88_000157"
     CDS             complement(18330..18842)
                     /locus_tag="KPB88_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052471.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEINGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19395..20276
                     /locus_tag="KPB88_000158"
     CDS             19395..20276
                     /locus_tag="KPB88_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG0052472.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDIFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(20589..21368)
                     /gene="hisJ"
                     /locus_tag="KPB88_000159"
     CDS             complement(20589..21368)
                     /gene="hisJ"
                     /locus_tag="KPB88_000159"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG0052473.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(21914..22264)
                     /locus_tag="KPB88_000160"
     CDS             complement(21914..22264)
                     /locus_tag="KPB88_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG0052474.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(22280..22696)
                     /locus_tag="KPB88_000161"
     CDS             complement(22280..22696)
                     /locus_tag="KPB88_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG0052475.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(22715..22906)
                     /locus_tag="KPB88_000162"
     CDS             complement(22715..22906)
                     /locus_tag="KPB88_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG0052476.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23057..23872)
                     /locus_tag="KPB88_000163"
     CDS             complement(23057..23872)
                     /locus_tag="KPB88_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG0052477.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(23919..24857)
                     /locus_tag="KPB88_000164"
     CDS             complement(23919..24857)
                     /locus_tag="KPB88_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0052478.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(24854..25726)
                     /locus_tag="KPB88_000165"
     CDS             complement(24854..25726)
                     /locus_tag="KPB88_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0052479.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(25751..27679)
                     /locus_tag="KPB88_000166"
     CDS             complement(25751..27679)
                     /locus_tag="KPB88_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG0052480.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(27791..28168)
                     /gene="gcvH"
                     /locus_tag="KPB88_000167"
     CDS             complement(27791..28168)
                     /gene="gcvH"
                     /locus_tag="KPB88_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG0052481.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28255..30381)
                     /gene="cdhC"
                     /locus_tag="KPB88_000168"
     CDS             complement(28255..30381)
                     /gene="cdhC"
                     /locus_tag="KPB88_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG0052482.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(30438..31244)
                     /locus_tag="KPB88_000169"
     CDS             complement(30438..31244)
                     /locus_tag="KPB88_000169"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0052483.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(31294..32661)
                     /locus_tag="KPB88_000170"
     CDS             complement(31294..32661)
                     /locus_tag="KPB88_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG0052484.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(32692..33636)
                     /locus_tag="KPB88_000171"
     CDS             complement(32692..33636)
                     /locus_tag="KPB88_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG0052485.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(33656..34426)
                     /locus_tag="KPB88_000172"
     CDS             complement(33656..34426)
                     /locus_tag="KPB88_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0052486.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(34482..35867)
                     /gene="lpdA"
                     /locus_tag="KPB88_000173"
     CDS             complement(34482..35867)
                     /gene="lpdA"
                     /locus_tag="KPB88_000173"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0052487.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(35911..36792)
                     /locus_tag="KPB88_000174"
     CDS             complement(35911..36792)
                     /locus_tag="KPB88_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG0052488.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(36828..37502)
                     /locus_tag="KPB88_000175"
     CDS             complement(36828..37502)
                     /locus_tag="KPB88_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG0052489.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(37539..38411)
                     /locus_tag="KPB88_000176"
     CDS             complement(37539..38411)
                     /locus_tag="KPB88_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG0052490.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(38503..39135)
                     /locus_tag="KPB88_000177"
     CDS             complement(38503..39135)
                     /locus_tag="KPB88_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG0052491.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39240..40916)
                     /locus_tag="KPB88_000178"
     CDS             complement(39240..40916)
                     /locus_tag="KPB88_000178"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG0052492.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41165..41944)
                     /locus_tag="KPB88_000179"
     CDS             complement(41165..41944)
                     /locus_tag="KPB88_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0052493.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42254..44173)
                     /gene="cooS"
                     /locus_tag="KPB88_000180"
     CDS             complement(42254..44173)
                     /gene="cooS"
                     /locus_tag="KPB88_000180"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0052494.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(44780..45691)
                     /locus_tag="KPB88_000181"
     CDS             complement(44780..45691)
                     /locus_tag="KPB88_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0052495.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(45807..46385)
                     /locus_tag="KPB88_000182"
     CDS             complement(45807..46385)
                     /locus_tag="KPB88_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0052496.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTIYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            46611..47534
                     /locus_tag="KPB88_000183"
     CDS             46611..47534
                     /locus_tag="KPB88_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0052497.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(47706..48848)
                     /locus_tag="KPB88_000184"
     CDS             complement(47706..48848)
                     /locus_tag="KPB88_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0052498.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(48866..50620)
                     /locus_tag="KPB88_000185"
     CDS             complement(48866..50620)
                     /locus_tag="KPB88_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG0052499.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            50756..50962
                     /locus_tag="KPB88_000186"
     CDS             50756..50962
                     /locus_tag="KPB88_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052500.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(50967..51890)
                     /locus_tag="KPB88_000187"
     CDS             complement(50967..51890)
                     /locus_tag="KPB88_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052501.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52144..53844)
                     /locus_tag="KPB88_000188"
     CDS             complement(52144..53844)
                     /locus_tag="KPB88_000188"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG0052502.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEEEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(54153..54566)
                     /locus_tag="KPB88_000189"
     CDS             complement(54153..54566)
                     /locus_tag="KPB88_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0052503.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(54634..57012)
                     /locus_tag="KPB88_000190"
     CDS             complement(54634..57012)
                     /locus_tag="KPB88_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0052504.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSIDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            57331..58782
                     /locus_tag="KPB88_000191"
     CDS             57331..58782
                     /locus_tag="KPB88_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG0052505.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(58986..61499)
                     /locus_tag="KPB88_000192"
     CDS             complement(58986..61499)
                     /locus_tag="KPB88_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0052506.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(61722..62543)
                     /locus_tag="KPB88_000193"
     CDS             complement(61722..62543)
                     /locus_tag="KPB88_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0052507.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63061..63537)
                     /locus_tag="KPB88_000194"
     CDS             complement(63061..63537)
                     /locus_tag="KPB88_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG0052508.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(63571..64149)
                     /locus_tag="KPB88_000195"
     CDS             complement(63571..64149)
                     /locus_tag="KPB88_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0052509.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(64253..66853)
                     /locus_tag="KPB88_000196"
     CDS             complement(64253..66853)
                     /locus_tag="KPB88_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0052510.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFRGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        20878 a        12567 c         7407 g        26277 t
ORIGIN      
        1 agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt tcaaaaaaaa
       61 tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct ggtaacccta
      121 gttagttaat atatctattc ccaataccct ttcataagga tatattttat atttattaaa
      181 aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc gagctctttt
      241 tttctgcaaa aaatcgtaaa aataaagtta atattaatat aactttaatt ttaaaattaa
      301 tattaaccat cactaaaaaa ggatgtattg aatttctaat atcaataaca tcctttttat
      361 atattatagc acttttgcta aaaatgcttt tgttctttca tgttttggat ttccgaaaac
      421 ttcttctgga gtattatctt caagtatact tcctccatct ataaaaataa ctctatctgc
      481 aacttcctta gcaaatccca tctcatgagt tacaattgcc attgtcatac cttctttagc
      541 aagttctttt ataacatcca aaacttcgtt taccatttct gggtcaagag cagatgttgg
      601 ctcatcaaac aacatcatat ctggttccat agcaagtgct ctagctattg ctattctttg
      661 cttttgtcct ccagataatt gtgatggata tgaatctttt ttatctaaaa gaccaactct
      721 actcaacaaa atttctgctt ttttttctgc ttcttcctta gtcatctttt ttaatttaat
      781 tggtgctaat gttatattat ctaaaatagt cttatgtggg aataaattaa agttctgaaa
      841 caccatacct atattttgtc taacctcatc aatattagtt tttttatcga ccaaattttt
      901 tccttcaaat attatttctc cacctgtagg tatttctagt aaattcatac atctaagaag
      961 tgtactttta ccagaaccac ttggacctac tataaccatt atttcgcctt tagcgatttc
     1021 taagtctata ttttttaata catttaaatc tccaaaagat ttacttaaat tttttattgt
     1081 aatcatatta tcactttcct ttttactatt ctatgttttc cctaaatgct aaacatgctc
     1141 tcatctagtt agacacagat agtttctttt ctaataatcc aactaatctt gatagagtaa
     1201 atgttataac aaagtaaatt gctgctgcta ctattaatgg ctctaatgct ctaaatgctg
     1261 cattttttac tatatcagca gtacgcatta tctcaacaac tcctattaca gatattattg
     1321 aagattcttt tacaagtgta ataaactcat ttgctaatga aggtaatata ttttttatag
     1381 cttgaggtat tattatatat ctcatagttg accaataatt taatcctaat gatctacttg
     1441 cttccatttg tccattatca actgattgta tacctgatct aagtatctct gccacgtatg
     1501 caccagagtt tactgataat gcaaatgcac tagctataaa ttcacttggt ataccaaaca
     1561 acatagggaa tcttattcct aattgaggta aaccaaacca tactatatat atctgtacta
     1621 ataatggtgt ccctcttaaa acctcaatat agattgatga tattgctctt aatactttgc
     1681 tctttgatat ttttgccata cagataatta tacctattat aaatccaaag cataatgcca
     1741 caagagaaat tgatactgtc acacctgttc cttccaaaaa agaagtccca aatctactta
     1801 aaaaactaaa atctaaactc ataatttcat ctcccacata actttcttac taatttgaag
     1861 cagcttcagt agatgcttca tttaaaaatt cttctatctt cttgccatct tgtaattctt
     1921 ttatcttttt gtttaataat tctataaaat ctttgttatt gtcagatttt ttgattgctg
     1981 ctgccatacc ttctgttaca tctttcccaa cttcagtatt tgccattttt attccatcat
     2041 attttttaac atttattaaa gaaactgctt cattagtaac aactgcatct atgtttccat
     2101 tttttaattc tgtaattaaa tcaggtattg ctttcaaaga ttttgtagtt gtcattttta
     2161 aagtgttaac tacatattct tcttggatac ttcctgcttg aactccaact ctaagtttct
     2221 ttaaatcatc ttcagttttt actttgtcta tatctgcatc tttaactata actgcatttc
     2281 tacttaaata atataattct gaaaaatcaa cactcttttc tctctctggt gttggagaca
     2341 tacctgcaag aacaatatca acttggtctg catttaaagc acctattaat ccatcaaatg
     2401 acatatcctt aatttcaacc tttacaccaa gctcttttgc aaattcttca gccaacatta
     2461 tatcgaatcc ttttattgaa tcttttccat ctactacctt atgaaattca aatggaggaa
     2521 attctgcact agtaccaaca actaattttc cattgtcttt tatttgttct aactttgttt
     2581 tttctcctcc accactacat cctactagcg ataatgtcaa tcctaatact aatcctaaac
     2641 ttaataattt tttaaatact ttcattttaa ctacccctct cttttaatta taatttccct
     2701 cagattttaa tacttaaatg tttcgagtta caaaaaaagt ctctcagtct ttaatgacta
     2761 agagacgact ttaccgcgtt accactctta ttgataaata cttatattac tttcaaaatt
     2821 tgtgactgtt agcttttaag ctgtatggat atttttattt taaaatcaaa aaagtccctt
     2881 agctttacag ctaagagacg aattatatcc gcgttaccac tctttttgat aagtaagtac
     2941 ttatcctctc aatcccttaa ggcgggtaaa ccaattatca tactaaagtt tctgataatc
     3001 tcctccagag ctctcttcac atagacttca ttgctaggct tacaccaacc cctaactcgc
     3061 tgtaaatcca tatatgctac tttctctatc attggatttg tttccttaaa ttgttaatac
     3121 aattgtactt ccttataata tttttgtcaa taagtttttt atttttttaa taatttttac
     3181 ttgtaaatta tctaaatact actgtttata tttattgata ttagttttgt aattataaat
     3241 agtatatttc atatttttag ctatttctta aataatacta aataatatct ttcacattgt
     3301 tgcttttctt taataatact aaataatatt ttccacattg ttgctttttc taaacaacta
     3361 taaataatat attaaaccct tatttctttt tctaataagt taaataatac attttacaca
     3421 tatctatttg tcttgcacat aataaataat acattatatt tctatctaaa tttagattct
     3481 aaattaaata gtataaatta tattcttcaa taaacaagtt ttcaaatcca ggattcgctc
     3541 tctatcttta aactttattg taaacataat tccatctttt ttaattattt ctccttcatg
     3601 aaatttttta tggtatacct tatctcctac accaatactg tcaatttctt ttttcgttgg
     3661 agattttata tcattaataa ataatgattt atcaactttc ttaccatact tatttaaagt
     3721 agagcttata cataactttt cttcagctct tgtaatagca acatacataa gtcttctttc
     3781 ttcttctaat tgttcttctt ttttctcttc cttacaaata tcatatgatt tttcatgagg
     3841 tattgttcct tcatttacac ctataatata aacatttcta aattctaatc cttttgcact
     3901 atgcatagtt gtaaatatga caccctcagt ctgcttattc ttgttatttt ccgttaactc
     3961 agtctttact ctatcaatat gctctaaaaa ctctgtaaca gtattaaaat tagttgctga
     4021 actctcaagt tcatttaata tctctacaag tccattagtc tttatctttc tgttgctaca
     4081 ataatctaaa acatatctat cataatcaag actagttcta atataggata ttgcattttt
     4141 aggatttaaa gtatttaaat aacttaaatc tatttccaaa tcatttattg ttttaacttg
     4201 tttaggatga agattacact tatttattaa tgctgttata aagtcatttt catccttaac
     4261 catatttata ctgtctttgg atatatatct aaaaggcttg tttataattc tcaaccaatc
     4321 tttatttgat tttggattaa tacctattct taaataggct agtatgtctt gacttgccca
     4381 atggtcatat attgtaatta ctgagtcttt tactacaaat ggaatcctca tatccataaa
     4441 tacatctact aatgctcttg actgtatatt agttctataa attactgcaa aatctgaata
     4501 ctctacataa tctttttgta tttcgtcaat tatttccctg gcaatatata aggcttcttc
     4561 ctctgaatca tgcggagaaa tataggtaac acttcctcca tccccctgac tacacttaat
     4621 gattttttca tatctatttt tatttttgtc gataagtcta tttgcagtat gcactatctc
     4681 acttttagac ctgtaattta tatctaatat tattttttta gtattattaa agtattgttc
     4741 aaattcaagc aaaaaatctg gtctagcacc tctaaatcca tatatacttt ggtcttcatc
     4801 tccaactgca aatatattgt ttaatgggct acaaattaac tttaaaactt caaactgtac
     4861 tttatttata tcttgaaatt catctatcaa tatatattta tacacatttc taaccatttc
     4921 tagtgcagac ttattgttca aaagtaaata ataagttctt ataagcatat catcaaaatc
     4981 tattttatta actttagatt tttgttcttc gtataagttg tatactttta aaaactcatc
     5041 ttttgttaat acttctgaat taaattcatt tttatccata agttcatttt ttacatagga
     5101 aatttcattt ataacttgtc ctatattctc atcatcatca gcattctcta tatttaaact
     5161 ttttaggatt gccttgattg tcattcgctt tgacttttca tcaaatatac tgtctaagtt
     5221 gtatctttca aagtatctta gaattttaaa aaatacagag tgaaaagtac cataggttac
     5281 cttattaagt cttatatcat cacttaaatt caaagctcta tttttcattt ctattgaaga
     5341 tgccttggta aaacttattg ccaagattct tgttggtgct atgtttttgt ttacaaccat
     5401 attggctatt ctatatgtaa ttactctggt cttacctgaa ccaggccctg caagtatcat
     5461 gcaaggccca tctatatgct caactgcttc taattgattt tcatttaact ttcttatatc
     5521 tatatttgtt gtgattatta gctccaccac cttgatttat aattatatta ttttcttttt
     5581 aattataaac taaaagttca taaaaataaa tttaattttt cctctattaa accacatcat
     5641 tcctattttc atgacatatc ttgagttctt ttttaatttt gtcgcttata tcttcaggta
     5701 taaatgatgg tttactaata tagtagcctt gcatataatc tactccaaga ttaattagtg
     5761 tttccatttc accttttgtt tctacacctt ctgctatcac tttaatattt ctttcgttag
     5821 aatatgagat aagatttttt aatattttct gtctgttttc atctttatca atacctctta
     5881 caatagacat atcaatcttt acatagtctg gcattatata taatagtaca gcatctccgt
     5941 tgtaacctgt cccaaagtca tctaaagcaa tttctatatt ccaatggtct atatatgctt
     6001 tctttttctc aataaaatct tcattaatct tatcattctc tgttatttct agtactactc
     6061 tatttaaata ttcaccatat aaatcttcaa tttcctcaaa atctactttt gacaaattat
     6121 tattaggaat agaatttaag aacagtttgc aatcgccaaa tttttctttg tattttttaa
     6181 atgatttaag tgctttaaac catgtaatac gctcaatttg atataattta gactgtgaac
     6241 gagctaatct aatgacatct attggtgaac taaaagtttt tattttaggt ctcataagtg
     6301 cttcatatgc aaatacttta cctttacaat ctacaatagg ctgaaatgca tactcaacta
     6361 attgaccatc aattaacttg tttaattctt ctctattact caataagaat gaatccttat
     6421 tatatatatt aatatcaaat tcactaactt cacccttgat tgtattttta atttgataca
     6481 tagcaaaatc tgcgtatctc attaattcat catagttggt tgagtcatca gggtaccagg
     6541 aaattcctgc tgatgccctt attttaaatt ctgttccatc atgaagtttt aataaggtat
     6601 tattcatctg tttcttaaca gaattaataa tatctcttat actatctttg ctttcatatc
     6661 catatataaa tacataaaac tcatcacctg aaagtctaga tactatacca ttccatgata
     6721 taaacttctt aagtatgtct gcagcacacc taatatattc atcaccagca tcatgtccat
     6781 atgtatcatt aatatatttt agattatcta aatcccacat aataaatgct gctatcttaa
     6841 gttcatcttt attcttaaat ttttcttgta tctgtgaatg aaaagctctc ctatttaaaa
     6901 gattcgttaa aatatcaaaa tctctttcat attctatctt ccttttctca ataaattctt
     6961 ttgtgacatc aacaacaaca ccaagaacct ttagctcatc ttcaaagata ttcattctta
     7021 tccacttaag ttttttatct ttagattcaa acttatatat atatgtattt tctgtttcat
     7081 aactttcttg caaatattta tctagtcccc ttaatttttt caggaactta tttttttcca
     7141 tatatccata ttcttcaaat ttgatttcag tagtaaacac gctaaaaaat tcttttgtac
     7201 aaaaaacatt ctctgcgttt ttgtataact caaaagcccc aattggcata tctaccattt
     7261 caataatctg tgataatttt gacgaagaat ctgcaacatt tgcactgagc aactctatag
     7321 ctgaagataa ttcatctatt tcagatatat ttatcttatc aagatttact ggttttgatg
     7381 gattactgtt tctaactttg ttggctaaaa gaacaattgg ttttgtaaat aatcttgatg
     7441 caatgtaaat acctattaga cctattaata aagaaattgc tatagatatt ataactaatt
     7501 tctctaccct ttttgaaaat gacaaaagac ttttttcttc aataataccc aaaagagccc
     7561 aacgttcatt ttcaaatgga gtattggagt tatataaatc taagtattgt acactagcat
     7621 aaatgttgcc attaccacat tcattatctt tatttttaat cttatatata tttttatacg
     7681 attccttctg ctctaaatta gaatgtaaac catcccaaaa tttttctttt aatgagtatc
     7741 catttgaaat tacattttca aaggccatat cattatttct atctacacct aaaatataat
     7801 tacccttttt attcccattt atctctgaat atggaagtaa ttctcttaaa taatttacac
     7861 ttaaatctat tccaagtact ccatatacag ttccatcttc ataaataagg ggaactgtat
     7921 atgtcataag cctatcacct gcatttgtca ataaaaactt acttccccag tatcctaaat
     7981 tagaacttga tacatttgga ttatcaattg cagctctaaa tggtttataa aaaaaatcat
     8041 ctccatcttg ctcattttca cttttaaatg aaaacttagg ttcccaataa ctgtccatag
     8101 atattccaaa acttcttgct atatctgatg aacctctctc aattaacaaa tcagaattat
     8161 catttggatt aaacttaggg tctaaatccc ttatatacaa tcctgtccta cttatttcac
     8221 ctttactatc tattgactta tcgttaatat ttttgtcact taggattata aaagctcctg
     8281 taacagaatt ttttctcaaa agatatatta aatcatttga tatgcttttt ataatatctt
     8341 tgttaatttc ttcattgttg cctacatcct caattaaggc atttttttca tttaatactt
     8401 tttttacaga ggaattaatt gtagcttccg tttcacttgt atttgaccat ctttgtaaca
     8461 tttcattttg tatataattt ttacgattaa ttactctttc atttaagatg tcaaacgaat
     8521 tatcatttaa tttcttaata gttcctcccc ataaaattgt ggcacaaaat aaacttgttt
     8581 gaattagcat aacaataatt aggggaaata tcatcttttt cattattgaa ttgttttttc
     8641 ttagattgtt ttccataccc attccaccta tacctttgtg ctcgtcaaca cactaactgc
     8701 caattcatct atttttttat agcattttca agctcagcct taaaactttc aaaccaagtt
     8761 ttaaaatttt tatcattatt aaattgtgct actgcatcct tcttagaagt tccacttttt
     8821 actaattcat ttattttatt tgaatcttct ttagctttat caagcattga atattcaagt
     8881 atttgtctag ctttagttcc accatcaaat gctttattag tatataaatt acaactgtta
     8941 atctgttcaa tagatgaggc tattgaacct tcaactttat cagataaact caaattatct
     9001 ttcttcataa cttcttttat aacatccaaa tcattcgttt ttttctttac tggtaagtat
     9061 ccagaagcaa tagagaattt tatattaggt tctttatctg taaaccattt taaaaattct
     9121 actgatgcat attcttcttc tttagtagat tttgttacag ccattcctgc accttgttgt
     9181 acagcatatt ttccttctcc tttaaaacgt ggtgcttgaa aaacagatgt ttcaatatta
     9241 taactctcat tatcatttag cattactcta tctggaaagt atgctgctcc tgaactagaa
     9301 cctacaagag ctaatatatc acctgttttt gcatcatcag aacgaaactt accataagaa
     9361 gcaaaatatc catttatata tggtacatag aaattatccc atagtttttt cataactttt
     9421 tcatcaatat ttagtgtaac tttaccatct ttaacagaaa aaatttctac tcctagttgt
     9481 ttacttccta taataatata atttgctaaa gcatctcttc caaaaaatgc tttaccatca
     9541 ttaggctctt tcgttaaact atctgtccat tcataatatt tttttgatgt ctctactaat
     9601 ccttcaattg tttttatatc atttatattt gaatttgtag cttttgcaaa tttatcccaa
     9661 tcagttttat ttaacatcaa aatttcagtt gattttgcaa ttgggaaaag tttaaactca
     9721 tccttagaat ttattctgcc ttcttctaca tattcgtcta tgtattcatt taattcttct
     9781 ttacttatgt acttatccat atctactagt aaccctaatt tatccatttc atatgcagta
     9841 tctgagtatg ctgcaaatat atttggtaca tcttcagaac caactttttt atttgcagaa
     9901 tctaaaactt tctctgataa atcaactaca ttaccttgac tgaatgcttc aacaataatt
     9961 cctttttcag cacctacagt gtcattaaat tccttaacaa gcttatcaaa agccattttc
    10021 tgctgtccat tataataatg ccaaatttgt attgatgtcg gattttctgg gtctaactta
    10081 taattatctt ccttctttaa actacatcct gtaagtaata aaacaatcat tgatgtgata
    10141 attaattttg caaacttaca cctattcata aaatctactc cttcaaatta taaaatttat
    10201 aatagcatta aaactgtaac ttcactattt atattaatta tagaacgttt attgatatat
    10261 ttaaacagga tttacaacat atttaaaagc aattaagaaa catactatta caaatataga
    10321 aaataaagct tttacataat ttttatttct aaatgcacca aatgacacca atccttgttg
    10381 aatagctact gtaattacta aaagtatcat tattatatta agttgaacca gtttaaataa
    10441 gtaaagtaat gaaaacacta ctaaaagtac acataataca atactagatt tattaataat
    10501 atcatcaatc ttatctacct tcttagattg cttattaact ttatttaatt tttctttatt
    10561 taaattctca acttttttat tatacctttc tcttgtttta tctttatatt tagtattttt
    10621 atttttattt cccatttatt ttccatcctc tataaatttt ttaattgagc ttatacatta
    10681 tttaaatatt atatatttaa gataccatat attcctacaa aaaggtaact tactatgtat
    10741 ctatttttga ttttcgcata aaaaagccat ctcctttaga attaattctg tattgagaca
    10801 gctaatttaa ctatttatcc tttgaaacat tttctacatt atcatattta ctatcaacta
    10861 aaactatatc aactcttcta ttttttgacc taccttcttc agatgaattt tctgctattg
    10921 gtctaaactc accatatcct acagcagata atttatctgg tgctatacct gcattgtcaa
    10981 taagaagttg aacaacattg gttgctctca taactgataa atcccaattt gacttaaact
    11041 ctgaattttt tatagacata ttgtctgtat ggccttctac tctcatgtaa ctattcattt
    11101 catttaacat cttacctatt tgaataatct tatctctaga attatccttc acaatcgctt
    11161 tccctgtatc aaatagtatt gtatctttaa ggcttattac taatcctcta tcttgaactc
    11221 ttaatgatac tgaattagcc ataccatttt cgtttaaata cttctggatt tcttcaccaa
    11281 tttttttaaa cttagcattt tgtaaagtat ctaaatcatt acttcctgct tcaactactg
    11341 gttctatctt gctattacca ccttcaataa ctccagaaga accaccaaaa gcagaattta
    11401 gagattgaga taattgttta tatttctccg catcaacatt actcatagaa tacattatta
    11461 caaagaaaat cataagtaat gttatgaggt ctgaataagt taaaagccat cgctcattgt
    11521 tttcttcttt ttcatcttca tcatacaaca taaacattcc atccttttat attattcagc
    11581 tctaccacca atttcttcaa gttcaattag ttcttttgaa tttaaaaata tttttaattt
    11641 ttctgtcatt aaattagaat tatcaccctc aagtatggat aatattgctt ctatcaccaa
    11701 aagtctttca cttacttctt gttgatttat agccttaagt tttgatgcta ttggtaacca
    11761 tattaaattg gcagaaccaa caccatacaa agtagccaaa aatgcaacag aaatttgagg
    11821 tcctaatgca tctggattac ttgaaagatt acctaaaata tgtactaatc ccataactgt
    11881 acctattatc cccagtgtag gtgcatatcc acctgctgaa tcaaaaattg ctattccttc
    11941 tttatgtcgt tttgatgtta tctgtatctg tgattccaat atatttctta tagtaactgc
    12001 atccactcca tctaccataa gttcaagccc ttttctaata aaagggtcta attcatcatt
    12061 ctcacttatt tctttgtcaa tactaagaag cccctctttc ctagcctttg ttgcaatttc
    12121 tttgaaatag attatatttt ctatgttatt aggcttttta tttttaaaag ctaataacat
    12181 tatctttata gactttttaa acacagggaa tggagtcgaa gctccaacag ctcctatagt
    12241 tccaccaaac acaacaagtg ctgatgaaaa tacaaataat gctccaacat gtcccccatc
    12301 aataacaaaa gcaactacca aagatgctat ccccactatg aaaaaaatta aagttgtcat
    12361 ctattccctc ctttctttag ttatacatat ctaatcattt atgacattat aaaaagacta
    12421 tctttaataa aaatagcctt taataattgg cggagaagga gggattcgaa ccctcgcacc
    12481 ggttacccag cctaatccct tagcagggga tcctcttgag ccacttgagt acttctccat
    12541 atctctatac tagtaaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
    12601 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
    12661 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
    12721 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
    12781 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
    12841 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
    12901 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
    12961 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
    13021 ctcaaacgac aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
    13081 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
    13141 gaatctaact gtaataacca actcaatttt agttgcaaac gaacttatga atactaatat
    13201 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
    13261 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggag ctggaggtat
    13321 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
    13381 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
    13441 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
    13501 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
    13561 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
    13621 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
    13681 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
    13741 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
    13801 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
    13861 attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca
    13921 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
    13981 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
    14041 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
    14101 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
    14161 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
    14221 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
    14281 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc
    14341 aatatagata taaattttcc aaaaactaat gacccattta tcataagcgt agaattaaat
    14401 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
    14461 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaagg tattgttatc
    14521 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
    14581 cctactggaa ttgaaaatac caatgtacat ccaagcatgg atgacacaac caatcctgca
    14641 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
    14701 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
    14761 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
    14821 aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag tccaaattta
    14881 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
    14941 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
    15001 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttggtt
    15061 ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat attttttgct
    15121 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
    15181 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
    15241 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
    15301 caacattttt atttttttcc ctgaggctaa attcatagaa tcaacaacat ctttgacttg
    15361 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
    15421 cccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
    15481 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
    15541 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
    15601 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
    15661 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
    15721 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
    15781 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
    15841 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtactcctat
    15901 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
    15961 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
    16021 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
    16081 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
    16141 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
    16201 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
    16261 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
    16321 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
    16381 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
    16441 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
    16501 gtcaattgct aatacatatt tcactttaaa tgtccccctt tattttcaga attaataaat
    16561 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
    16621 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattaaaaaa
    16681 gtatttttat tgaaatatta ttgcaatatt tattatttta ttctataatc tagttaaaat
    16741 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
    16801 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
    16861 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
    16921 gaatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
    16981 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
    17041 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
    17101 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
    17161 cccggaccta gacattttag aaaaatacat aattgaaaat tctgatataa aagtaattgc
    17221 tatggtgttc catgaaacta gttcaggaat gataaatccc gtccctgaac taggaaaact
    17281 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
    17341 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggta aagctcttgg
    17401 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
    17461 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
    17521 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
    17581 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
    17641 gaatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
    17701 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
    17761 aagggaaagt ggctatgttg tctatccagg aaaaggcaaa ttcctagatg ctaacatatt
    17821 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
    17881 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa ccctacaata
    17941 tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta
    18001 tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc
    18061 cttcagtata atctttttct tttaatttat tttatatttt tgacgcaatc gttttttatt
    18121 caatcatttt atattcttga tacaatcatt cttacactta attatttttt gtttttgaca
    18181 caatcgtttt ttattcagtc atttaatatt ttcaacacaa tcattcttac attctattat
    18241 tgtttgtttt tgatacaact gtttttattc agtcatttaa tattctcaac acaattacct
    18301 tttcattcaa tcaaagtaat ctttaagcat tatttttcaa gctcatcaaa gtaattatat
    18361 acgataacct cacctgtctc tttatctgta gtctttataa tgcccttagc tccatttatc
    18421 tcttcttttg taaattcatc atctagcaca actttgaatt tatcttcctt tccataacct
    18481 tgttgaaatt catctctcaa aattgcattt ttttctgctt ttggcttcaa ctcaaattca
    18541 attccagatt tcatttcatc aaaataattt aatactacaa cctcattatt cattttatta
    18601 gttgtcttta taatcccttt agctccattt acttcttctt ttgtaaattc atcatccaat
    18661 gttatattaa atttgtcttc ttttccatat tcctcttgaa aatccctctt catttcattg
    18721 tatttagttt caaactcttt actcttgttt aattccatag ctgatacatt tgtcaatgat
    18781 gctccaattg ccattactgt taatgccatt actataccac ttttaaattt ttttactttc
    18841 atatcttacc tcctaaatta aatgttctca atgaaattat ataaagaaaa tcctaagttt
    18901 tacagtttca ttcttgtaac taaaattact attatgtaac caaacttttt gcttcaacta
    18961 ttccttaaaa gttgcttttt tgaggaatag ttgaagttaa ataattagat tttatctata
    19021 tttatagatt taaaatcaaa tttatgcata taaataaata cttatttttg caaaataaaa
    19081 ttttttaatt actccaatcc ttcttattta cttaaaatcc tatgataaga aatttaaaag
    19141 tctttacctt ctttaacaat aatttattta cttttaacat acaaaatata gagagtatat
    19201 caaaactatt ttactgaata ttctaaaaat aagtaccccc tattagtata tatatttaca
    19261 tattaaaaaa aagttttaaa taaagttata ttttttacaa cagttttgta attaaaaaca
    19321 tattgaaggt aaatttaaag gtactatata ctataaatat gtcgacacaa taaatttaaa
    19381 aaggagaact aaatatgaaa aaaagattaa taataatgat attgtctgtt gtcttagtac
    19441 tatcaagtat acttacgata tttgcatatt caaatattaa atataataat aacaaattga
    19501 tatattctaa tatgatagat aaaaaaactc aaaatagtgt aaaggaaatc ctaaaagaaa
    19561 ataaaattaa tgaaaaagac atagatatat ttattaaagc agtcaacaat tataataaat
    19621 tgcaagttaa aatacttcaa aataatataa atatatctaa atctggatat tcttcaatca
    19681 atgcaaaaca agttccatat aacctagaaa aattgcaaga taattgggtg aaaaaattcc
    19741 ctgattatat ggatgtaaat tgtagaataa ctgcttttag actttttaaa gattttataa
    19801 attcaaataa gaaatttact ggagactcta ttgacttgaa tgtcgacttg gacactataa
    19861 tgaataataa agatgctaaa tttagcacca aagatgtaga aaaatttatc aacttctttt
    19921 ctgcaatacc agctaaagat acagatgata caatcaaaat tgcagaacaa ataaaaaatg
    19981 aatggaaaaa aagaaaaata tcttttaaaa ataataaaaa tatatctatt ataaatggat
    20041 ttcttcgtta tccagaaact aaaaatgtat ttataggtca tacaggcata tgtattaaaa
    20101 ctaaaaatgg cattctattt ttagaaaaat acggtgttac atcaccttat caagttacta
    20161 aatttaaaaa taaagaagat gtaaaaaatt atatgtttaa tagattgaag atgtcagaag
    20221 gtgaaattga attacctgac cctataataa tggaaaatga taaacttatg aaataataac
    20281 tttaaacgca aaatagtatt tataattact aagtcaatat aatagagaag gtctatagat
    20341 ttgtgtaaat acaagcttat ctacaatctt atataagaat gtttatataa ataagataga
    20401 ttaattctaa gtcctattat tctattgaat tttattatta ttttatttag attctattgt
    20461 tattttactt agaacttatt attattttat ttaagttcta ttactatttt caatcatata
    20521 aatttacaaa agactgtaca taaatttatt ttatacaaac ttatctacag tcttttgtgt
    20581 gaatatatct aaataatatc gtatttattt atttcctctg cacagtgaga atcagaacct
    20641 aaaactaatg gaatatcgta tttttttacc aactctaata agtatccatc tatataaggc
    20701 tctttgcagt actcttttct atttccagaa acattataat caagttcata acctttttct
    20761 ttaattaact ttattaactc ttcaagaatt gtatttcctt tataatcgta tggaaatacc
    20821 tgattaaact ttctaactaa atttaaatga cctattcttt ttggtttatg tatacctaaa
    20881 tctgaattta cagcttttat caatgtttca taatacttat tataaacagc ttcaatactt
    20941 cctaatttgt cgattaaatt ttgaaactct tctacactgt aatctacaca ataataatca
    21001 tcatcaattt taattatatg tactgataaa agagagtctt ctaattcctt tccatattta
    21061 tttaaattct cctttatccc ttcttcgtat ccttctataa aatccacttc cgacccaaca
    21121 tttatcttga ttttaccctc atacttttct ttcaacttct tgacatcatt aaaatacttt
    21181 ggtaaatctt caatactcat acaactatca ttttcaggag cagggtctct aaatccattt
    21241 gcatacggaa aatgttctgt aaatgtcatt tctgttattc caacatccat tgctctttgt
    21301 acatattttt caaaatcatc cttacttcca tgaggacaat aaggtgaatg tatatgacca
    21361 tctttcataa ataattcact ccttttttaa agaaaaaatc taatataaat gcataaattt
    21421 tttaacttca aaataatatt tttaatcatg ctaattttta ttatatctaa tattagagta
    21481 attttaaact gccttttaag tatcttatca ttagtactat tttcaactta acattaatat
    21541 tattttttaa cttaacatta atactatctt taaatagtaa tactcaatat ataattactt
    21601 aacataaaat aactcaaatt gcttgatgag gcttttgcaa tatttaaaga atatataatt
    21661 atttaacatt aaattatcca atcttgtaat aaaatttact cattaaagta tattacagaa
    21721 aatacctcaa aataaacttt ttagttcatg atatactctt ttttgcatga aattagatat
    21781 atttttatca tttcaacaat gaaagggagg cttatatcca tctttagaca accccttaga
    21841 taaaattttt aataattaaa catcacaaaa tataaaaatc gcttatatca aacgtataag
    21901 cgattaatta tatttacata tgttttttag cctctaacaa cattttatta gcttcttcca
    21961 tatactctat agccttttca atactctttg ctgtttcttc ttttccaata gctctagcct
    22021 tttcaaccca ctctctaaat ccttcttcat gagtttcatt gtgatttatc caatgtacta
    22081 aaagtatttt taaagttttt tcatccttag actctgatga ttcctcatga ctatgttcgt
    22141 ggtcgtggtc gtgagcatgg tcatgtggat gttcatgaca tatatctcca tgagtatgtt
    22201 cgtggcagtg gtcatggtca tgatgtccat gatgatatct tacaatctct ttatcaaaaa
    22261 tcattagtta ttccccctgt tattttatat taaactcttt ttttataagg tttacagaat
    22321 cttttacaag tagtgctaat ggtttacctt ctaatccaac taactccaca ttttctggca
    22381 taataggtaa aagtatctta agtgcctcac tgtctgcaat agcttcactt atattacaag
    22441 taacttctcc catcatagaa ttaggtatta ttacagaaat aggtgcaact attatatttg
    22501 cttttttagc tgatactact atagcatttt cccctgtagc tcctttatta gcatgagctt
    22561 tcatcataga actagttgct atagaattag tccctagtgc ataaatttct acatatgtag
    22621 gtaactcttc tctcaaagaa gaaactatct gggctccaat acctccaccc attccatcta
    22681 tcactgctat tatcatatga aattccccta ttaattaatt ttcttgtatt attatcttat
    22741 ggtccatcaa tcttatttct ttaattatcc catctattat cttttgctct cctaataaat
    22801 ccgttaaata aatctttcca tcacaagggt ctatattaac cacattttcc ataacctttt
    22861 ctaactcatt attactttta caaatgaaag ctgatgattc acacatataa atctcctcct
    22921 aatatgattt ttattaaaaa tcaaaaaaag attcccttag gtatttcaat acaccaaaga
    22981 aaccttcatg atttcagttt ttgtatatta ttatacctta agtatatcac tttaatttat
    23041 gtaaagtcaa ttaaagttaa cgctttttat tatactcttc attcttgtat ttttcatata
    23101 gttctttaac taattgcttc tcataatcag tcaaatcgcc ctccacaaac tcaacattaa
    23161 attgttcagc taatcccttt actatgtttt tctgtagaat atctatgtct atttttctgc
    23221 caatttcatt ttctattcca cttgcttttt tagctgtaaa tttcatagct ctttctttca
    23281 attctggtgt cttagttttt attaatttaa acaatttttc tacatcaaaa tccagtatta
    23341 ttgaaccatg ttgtaataaa acaccatctt ttctgctttg agcacttcca attaccttct
    23401 tattattaat agtaacttca tatgatgcat gtgcattaaa acatgcagct gataaatttt
    23461 ccctacttat tctctcgcct ctatttaaat tatctgtttc tataccactt agattcaatc
    23521 ctttaactag accttcactt atatatctat aagataggtt gatgctctta tcgattaaag
    23581 gattatcttc tcctataatt atactatagg ttagctcatt atcatgtaat acagctcttc
    23641 cacctgtaat tcttctagta tagtctatat tcatacatct acacttatct aaatctatct
    23701 catcttccaa tttctgaaaa tatcctatac tcaaacaagc tggttcccat gtataaaatc
    23761 taagagttgg cttattatgt cctttcttat atgcagtaaa tatagcttcg tcaattgcca
    23821 tattcattgc tccttcatat gatttattat gtataactct ccactgattc atgattatta
    23881 cgcccccatt tttagtataa atatatccta gaaatcctct atagatttat ttctcttatt
    23941 gctaaatcta tctcactttg atttaaaggt cttgaatagt tgtatatagt acttcctggt
    24001 ctttcattat agtatggtct attacaatcc ttacaacctc ttatttcaaa aggatagcct
    24061 ttgtttatgt cttttaaaat atcattgtca agctttatac tatttaagta tccattttta
    24121 aacttaaaat actcttttgg ataaccttta tttatcatat aagacattaa ctgtaccctt
    24181 ctataacttt ctatgcttgg ttgatttatc ttttccattt tagttcctct aactggagta
    24241 aatgcaaata aactaattgt tacatcattt tcctttaaat aagtatataa attgtatata
    24301 tcttcatgac tttcacccat accaactatt atatgtgtac ttattttatt gggatatgat
    24361 tttgacattt cagttataaa ttttaacttt tcatcatagt tatttccttt tattttttca
    24421 tatagttctt tatttgctgc atctattgct attcctattt tatcaactcc tgctgaaaaa
    24481 aacttcttta tctgttcatc attttctaat tttgcagata gtgaaattgg catgtctatc
    24541 ttaccactta tatagtttat aaagtctaaa acagagttat gtgcttcttc acttgccatt
    24601 gattgaatac atattctctt tatatttttt cctttataag cttttaaagc atctaatatt
    24661 tcttcttttg aaaactctgg ccaaattact cttgataatt tatcttttct agtactactt
    24721 tctattgatt gagaacaaaa actgcattta tttatgcatt tttctccaat cattatatat
    24781 gctgtggtag gaggtatatc gctttttttg tttaatatcc caagttctat tgctgtccca
    24841 actgatagtc taatcataaa acgcaacact ccttacactc atttattgtc atattcattt
    24901 ctatagcctt attctttgca gaccttgatg gtaatactat tctattgact ccacacatta
    24961 tagataattg gtctagctct tttctataca ctcctcttgg tctcatacaa cctagattaa
    25021 tttcagtatc tggtaaatta attctagctt cacataaaat atctgctacc ccttctaact
    25081 caggaggttc tacattttca tactcagtcc cttttgttgg aataaggact ataaatgtaa
    25141 gcttattagg aggatttttt tgtaggtatt ctataatttc atattcaccc tttatttgtc
    25201 ctccttttaa accaatacat atatgtggtg ctacttctgt atgttcttgt attgtattat
    25261 agacttcaat ataatcttct ttactctttt tcatcttata aacttctctt attgtctcat
    25321 catcaaacac taaatcaaaa gaaactatat ctaaatactt acacaactct acaatgctat
    25381 ctttatccat aagacctaaa tgagcattca atctatatcc ttgctctttt aagtttttaa
    25441 tggtattaat atgagtattt atagggacat ctccctcata actacaaccg ccactcaata
    25501 gaaagctagt tatattcctt gattgtactt tttcctcata ttcttctatt ggcaccatat
    25561 tgtttaagta gtgaccgttg caatgggcac atttcaaact acattgattg cttgtagtac
    25621 taagagctag tgtctggtta ggataagcaa actctatttt atttccaaaa ttctttaatt
    25681 taatatcaaa tgctattttc attttttcag atagattctt attcatagtt tattccttct
    25741 taagtacatt ttactcttct agtaatttct ttgctgttgg gaactgctct atattcgtat
    25801 gtggtaagaa acatgcagaa ataaattcat ccatataact tggatataca ctaagttcaa
    25861 cataagttat ttgattacct atttcttcaa gtttattttt tgcatcttta cttatgagag
    25921 ctaagtaaga accaacaagt gaactattcc ctatataagt aaatttttct ctatcgatat
    25981 caggaagtaa ccctataatt attgagtttt ctatgtctag gttgtttcct ataccacctg
    26041 ctatataaac cctatctaat atactaaaat ccatacctaa actatctaca agagtgtatg
    26101 cgcctgaata tatagctcct ttagctctga tgaaattatc tatatcaact tcattgacta
    26161 ctatatcatt ttctaaatca aattcttctt taaatgctaa tacatactct cctatctcat
    26221 gctcattaaa tctaactctc ttattatcta aatccctgta tattttacct cttctatcta
    26281 ttacaccttt agtaatcatt tgacagatta aatcaattat accagaacca catataccaa
    26341 ctggtgcaca ttcatcaata atctttaatg ttggctctaa agtatcttta tcaataatta
    26401 ctttctctat tgctcctgct gatgctctca ttccacagct tatccctcca ccttcaaaag
    26461 caggtcctgc tgaacatgca caactcatca tataatcttt attaccaaat acaatctctc
    26521 cattagttcc taaatctata aataaaacat tttcttcact tgaccatatc cctgctgata
    26581 atactcctgc tgttatatct cctcctacat aacttgcaac agatggagct aagtacacat
    26641 atgcactgtc atttacaaaa agaccaacat tttcacccat aagttttgga gattttaaaa
    26701 atggtggtat gtaaggttct tgtcttaaga agtctgtata cacacccaag aataaacttg
    26761 tcatagttgt attcccagca gccactaatg taactacatc ttccttatct attccatttc
    26821 tttcatatat tgtttttaat agtggattta tagtttcctc aactatagct ttatgaagtg
    26881 tctctaatcc atttttctta gttgaatata taattctatt tataacatct gctccatatt
    26941 ttatctgtgc atttccagaa gatgctttat ctactacctc ttttgagtat aggtctacta
    27001 aacaaacaac tactgaagtt gtaccaatat ctattgcaac tccgtataag gaattttctt
    27061 tatttccttg ttctatattt atgattgtaa gtttcttttg tttttgaaca tatgttattg
    27121 taaccttaaa atcactcttt ctaaatacag ttggcatttt tctaagtata tccaatctaa
    27181 aatctatttc attatatcct aggttatttc taacatatct ttccaatcta tctacatcac
    27241 ttatattatc atctaaattt ggttcttcca tttctatgta tttctttttt atatttgttt
    27301 taaattgaag gttatgttct tcaattattt ttttagctct ttcaaaaatt tccctatcct
    27361 cttttttatt actaccttct atcttcatac catgcataga agatgatact ttagaaggaa
    27421 cttcaatttc tatatctgat attacctttg tacaacaagc tagtatataa ccttgctccc
    27481 actcatcatc tgttatatgt cttgtttttt ctgtatcaac ttttccattc aataatttta
    27541 ctttacactt cccacaagac acgtttccat tacaaggagc atctatgaat atatctgcat
    27601 ttcttgcaac ctctagtaat atatccccct cattacaata aacctcttta ttgtttggtg
    27661 taaaacttac ttttatcatg ttatccctcc caatttgttc tatatatgat aaaaggaaag
    27721 tatagctaaa tttgcgtgca ctcaaatttt actatacttt ccccaattat aataatatct
    27781 taatctttaa ttattctaat ccagcctcgt attctttatc acttaataac gtatcaagtt
    27841 cagccacatc agctagtttt acttttaata tccagttttc atatgggtct tcatttatac
    27901 attctggctc atcttctaat ttttcattaa cttccaatac ctcaccatta acaggagata
    27961 ttaaatcaga agctacttta gaagactcaa ctactccaaa atctactccc ttagttacag
    28021 tatcttctac ttctggtgtt tcaacaaaca atatttctcc taattgatct tgagcatagt
    28081 ctgttatacc tatatataca acatctccat caattacttt tacccactca tgatctttag
    28141 agtattttaa ttctggtaat aatttcattt cgatagaccc ccaatattat attattgatt
    28201 aaattactta aatcatatta atattatatc atattatttt aaattttcca tcaattacat
    28261 tacactttcc atagctaatg ctggatgacc tttttcttct aagaaagcta atactccttc
    28321 agaatcgtca gctatagttt catcacaaac catatcacag aagttttcta ttccagtcat
    28381 ttctttaact gtagcattta atttatcagc tacataatct tttaattctt ttggcatcca
    28441 aactattctt tctggtccac cttctgcata agcgaatttt ttagaagata tgaaatgtct
    28501 tccgtgtccc ataaatcctg gagtttgaac tcctccacct gtcatagaag caagttctcc
    28561 gaaagtcata ccaactggag taacacttgc aaattctctg ttaactacaa caaatccatt
    28621 tgcttctggc attataccac agatacattc gaagcatcca caagaagtca ttgggtcttc
    28681 tagtatagag tataatgtaa cagattcaac tgcaccttga gatatttggt ttacagtttc
    28741 atttactgaa ttccatacac ctgttctatc atctaagcac tcgccttttt ctattggttg
    28801 acaaggacct gttgggtcta gttctttagt agccttagca tctaaccagc taactgctcc
    28861 acaaagacca agtctttcag gagtaacaac acaaacgtga gctggtgcaa atgattggca
    28921 taagttacaa gaatagaaag tatcaacact ttcatcaact aatgaagcta atctttcatc
    28981 tctagcattg tacttagcta ttgcttctcc ttttaattca gaaacttttt cagcatctgt
    29041 aattatagtt atttcacact tatcaactac tgattcaaat tcatctaaca tcttagcata
    29101 taaaacttct cctatatgct caagtctaaa tcctttatca aatgcatctt tagagattct
    29161 tacccaagcc atatctcttt gtccaacgtg cattacacct tctatatagt ttaagaagta
    29221 gtggaaacgt ctttcaagaa ctggctcgaa atcttcttgc atatttttac cagctatttt
    29281 aactattaca gcaagtggta atcttaatac accatctgca tcaacttcat ctatatttgg
    29341 tcctattatt tctatcttat ggtcttcaac ttctgttgct tctttcttaa caacaagctc
    29401 ccaagcttca gttctgtttc caccaaactc agcaaacata tcgctctttc taattctctc
    29461 accttcaaat gctgctgcaa atgaaactgg gataggtatt tcagtaactt ttatttttat
    29521 tcctctagct tctaatgaag tagctactat cttatcgtaa tctttttgag ttaataggtt
    29581 cattggaact tcaagaactg tttggtcagt tataactggg aatcctaaag ctattgctcc
    29641 agcacctgca gatacaacta attcacttaa tggtccaaat gcattaacaa atgcaggaac
    29701 tctattagct gtgtactcta ataatccgtt taaatcacca ggagttaatc caccgaatat
    29761 taatgctgct cttactgcaa cagatacaac gtgtataaca gaagttacat catatcctag
    29821 aggtataact cttagctcaa gacccatttt tactccagct tctatagctt ggtctataac
    29881 tttaccaact aagaaagtta gtaaaccttt agattggtaa tcttttataa cttttgctgc
    29941 tgattctgca tctggacact caccaagaac aactgcaaca ccaggtatat ctcctgtaac
    30001 taatggtaca ccaagtgatc tgattatagg gtcagttata tgacctgcac atggctcact
    30061 atatggagca tccatagttg aatattttaa tgcttcaatt acttctgctg ctaaagctgt
    30121 agctaaacca gcattaaaag catgttctag taaatgagtt ctatttatta aagattttac
    30181 tacttctaaa gcaccttcta agtcccctaa agtgttcatc ttttgtcctg ttgcagcata
    30241 tatacaaggt aatgaatatg ctgtatcagg gaaagcaact ttatgctctt ttccattctt
    30301 ttctatagct tcagctaaca tagcttgagc cgcacctaaa gcttgttctg accctgtaaa
    30361 gattatatta tatagattca ttctatttcc ccctttaata tttacaccta ttttttatta
    30421 actataccct aaaaacccta gaaagcaaat ccaccttcat ttaactctaa aaaagagtct
    30481 gcatataaag atgccccgtt gattatgtca caagatttta cagttttcat taattctggg
    30541 tcacatggat tcattatagc tgaactaaat ccattagcca ttgccattgc taagaatgca
    30601 ctatctaaaa caggtctaac atgcttagga catccattag atacgttaga taatcctcct
    30661 gtagttaaaa gtcccatctc agtcatcatt tttatagctt ctaaaacttc tacttgtttt
    30721 tcttgcatac cttttataac taagcataat gggtcaaata gtatatcagt tggctccatt
    30781 cctaacatta agcctttttc taatatttct tggcagtaag ccatacgctc atcattatct
    30841 cttgggatac cttcttttgc acataaacct ataaccattg cttcgtattc agctgctata
    30901 tctattaaat caaatcttga gccagcatca gcagagttta ttattggttt tgcattagtt
    30961 ctatcataaa ctttaagtcc agcttcgata gcttttttat tagctgtatc taatgctata
    31021 ggaacattgt tgaactcaga ttgaagtaat ttgattcccc atgtcattat ttcttcgcca
    31081 tctctttcgg caggtcctat attaaaatct atatagtgtg ctcctgcttc taattgctct
    31141 tttgctcttt ttaagattgg agctgggtct ctttctgcta atgcttttct tattgaaggt
    31201 gatatacaat gtattctctc acctataatc ataaattttt ccatttataa tgtccccctt
    31261 taaatttaat attaatagtt agttaatatt ttattagttt gtttggcatg ctttttcttg
    31321 gtatcctttt aagaacttag gtaattccat agattcctct ggtcctataa ctacattcca
    31381 tcctggtaag ttatcttcta tatcaccttt aagtactgca acttttcctg gaatgataag
    31441 gtctttacaa ttagttactt cttctacctt actttcttta atgaaagcag agattgagtt
    31501 tccaccaaat ttaccagcag cccaagatgt aagaactgaa tatccacctg catctggtat
    31561 tactaaccat actggaactt ttgatctttc tatatcacca gcaactatga agtaagttaa
    31621 tgcaaagtca acagtaacta atactggaga attttcatct gggttgttga ttggataaat
    31681 ctttggttca actctcattg gtctttgtgg gtcagtgtat atgttttgtc ttagtgcaaa
    31741 taatggtaat gccttagcat aactaatatc atctattact attatagaac cgtattttat
    31801 agtgaatatt gatgataaag caacttccat catagggttt gaattagata atctatttgc
    31861 aaatactatt gaaggatatc caaatgttct atcttgctct tttaaagctg ttcttctaac
    31921 ttgtatagca ttagtgtaag tatcttttat gttttctcct gttacatcta atactaattc
    31981 tttatatcca gcagcttgaa ttaattcaac tgtttcatat aattcttcta agctaccagc
    32041 ttttacacct aatggtaaac ttgctccttt aactacttct atcatatctt tgtagttctc
    32101 tttagttgct ccatatacca ttggtctagc atcttttaat acttcaacag cttctttaac
    32161 tacttgtgca tcatcacaag ctaaaatata agctacttct aatccactgc cttttatttt
    32221 atctattaat tttaaatacg catctttatc tccacaatac tctaaaacag ccatttcaac
    32281 tttcatttgt tcaccaattc ttacatagtc aacttttttc atgttagcta ttttagcatc
    32341 tactgcttca tcactcatac aagtacagaa tgaaactgca tatctatttc tacttactaa
    32401 tgttttttca tgtctaaata atactgtttc tccacctagt tcatactcag atgcaccagc
    32461 accaactttt aaagctttca ttaaaggtgc agtagcctct gataattttg ctatagcgtc
    32521 atcagacata tgtgggcatt tacctacttc tacagctcct gaagcaacct tcatagagaa
    32581 agccatacaa gttgggaatc cacaatcctt acagtttttc tttggtgtta atttaaatat
    32641 atctaaagct tttaatgcca ttattttttc cccctaacct taacaattta attaagctaa
    32701 tgcgttaacc aattctttta tagtttctat tgattttgga tgacgaagta taactgcgtt
    32761 tgaaccacct actaaaacac ttgctgcagt tgaaacttcc attgctatag ttctttcttc
    32821 tggacaaccc caatctggct catcttctat tggagctata gcttctttaa catgacctac
    32881 ttcaaaagct actggtgtta tgataggcat ttgtaatgtc ttatcatttt gaccaaatgc
    32941 tgcaagtcta attctatcca tagtagatgc aacgtactca tatccataac caactgctga
    33001 acatccaaca ttcataacta tattttcacc tttaacgcct agttgagtta acaatacgtt
    33061 taattgttta gctaggttta tatcaacaga agattcagct cctactttat gtgcataagc
    33121 cataccagct gatgctccta ctcctttgta gttgtcttct actgctgaca taaataagca
    33181 gttatgtcca tccaatgttt gagctaattt ttcaaataat ttaccatctt tttcatgatt
    33241 tcctgaacca gctacaacta aaggtaattt tattgcttca attactgcct tagctacatc
    33301 agcacattca tcaactgact tatctaatcc atttgggtca gaaccatcaa attttaaaca
    33361 aataaaatct gcttgtgtat tagcttcaac atatttagcc cactctactg gacaattagc
    33421 tacatctttg tataattcct tatatgaatc agtccagctt tcaggataaa cgtctgatat
    33481 ttgtatccct atttttggag aattacctac ttctccatca aaactataaa aaggtaatac
    33541 attttcccca cctaatttaa ttgctttttc ccctattcct acttcaactt ctgatatttt
    33601 tccagaatat ttttgagtag acattttaaa tgccatattt ttttccccct taagcctatt
    33661 ttaattctaa tttagcaaaa atttctttca tagccacttt agattttgaa tcttcaggta
    33721 aattaactaa aggaatacct gtagaatcat attcatatat caattcatcc ataggtacaa
    33781 caccgattag ctctaggtta tgcttttcaa tttcttcttt aacaccatca ttcataaccc
    33841 catttggaac tttatttaca atcaaaagta ttcttccaac acttaacttt agttcctcag
    33901 ctaaatctct tattctagca acagcttgta tgctacgtct tgagcaatca ctaaccaata
    33961 aaagtgtatc aacatgtcta gtgacttttc tacttagatg ttccatacca gcttcgttgt
    34021 ccatgactaa gtatttgtaa tgaccagata ttttgtttac ttgttctcta agtatcccat
    34081 ttacaaaaca gtaacatcct tcaccttctg acctacccat tactaataag tcatatcctt
    34141 cgccttcttc gattatagaa tttaatctaa attgaagata ttgtgccttt gtcataccac
    34201 cagggaatgc attgcctaac ttttctcttt gatttacttc ttctcttatt tctcctattg
    34261 ttgcttcaac ttcgatacct aacacttcat ttatgttagc attggcatca gcatctacaa
    34321 ctaatactgg tccctttttg tccttaacca aataatcaat caaaagccct gtgagacttg
    34381 ttttaccagt tccacctttt cctgcaacag ctatattgta tcccatttta agacttcccc
    34441 cttaaatcta gccacagagc cttatgtact ctgtctgtct attataattt aggtacagaa
    34501 tgaacacatt ctccatgtac atcatgaagt gcttccatta gcccttcaga taaacttgga
    34561 tgtggatgta ttgcatcccc tacttgttct actgttaatc ctagatgaac agcaagtgac
    34621 aactctgtca ataaatctgt agcatgaggt cctactactg ctgcacctat tatcttatca
    34681 gtttctttgt cagctataac ttttacaaat ccttggaaat gtcctatagc ttgtgctttt
    34741 ccaagacctc taaaatcaaa ttgacctaca ttgtattcta caccttcagc ttcaagttgc
    34801 ttttcagtct taccaacacc agcaacttca ggttcagtgt aaacacatct tggaattgct
    34861 ctataatcta ctaccttagt cttacctaaa gcattttcaa ctgctacgat accttcttta
    34921 gaagcaacat gtgctaggaa aggagtatct attatatcgc ctattgcgta tataccttct
    34981 acattagttt caagatgttc attaactact acttttcctc tttccatttc aattcctata
    35041 tcttctactc cagaattgtc aaggttaggt cttctaccaa cacatactaa tgcatattgt
    35101 gcttctataa cctttccatt agaaagagtt gcaacagcct taccatcaac tacttcacaa
    35161 gtttgtactc caatacctgt aataacctta attttatctt tcttaaattg tctaagtagt
    35221 tgtttagcta catctttgtc ttcatttaat agtatttggt caaccatctc aactatagtt
    35281 acttctgttc ctaaggctct aaagaactgc cctatctcac atcctataac tccaccacct
    35341 actattaaca tagactcagg tatctcttca agtccaagaa cttcatcact agttattaca
    35401 acttttccat catatgggaa cattcttggt acaactggaa cagaaccatt tgctagtatt
    35461 atcttatctg cttttatagt ttctactgtt ccatcatctt tagttacttc tatagtgttt
    35521 ttatcaatta attttccaaa accattaact aagtttactc ctcttttttc aaataagaac
    35581 tctataccac ttattaattg gtttacaact ttattttttc tttccattat ggcagtgaag
    35641 tttggcttaa ctgtaccatc tatttctata ccaaaatcct ttgcttcttt tacagtattc
    35701 aatactcctg aagaagcaag aagtgcttta gttggtatac atcctgcatt taagcaagtt
    35761 cctccaactc ttcttttttc aacaactgtt acatctgcac caagcataga agcttttata
    35821 gctgctacat atcctcctgg tccaccacct actactacta tcttcatctt taattccccc
    35881 aaacctaata taatctacaa tcttgcaata ctataaacct gcggcatcca gtatagcagg
    35941 tatattctct tctgccccaa ttgccattat atgaacacca tcacataaat cttcttcttt
    36001 taattgtttg ataagttccc ctgccatttt tattccttca cttacccagt tttcttttcc
    36061 agctgctctt aatctttcga tttgttcatc tggaacaaat attcctggta catttgcagt
    36121 cataaattta gccattcctg gtgattttaa aggtactatt cctgctaata ccttgcaatc
    36181 catatccctt gttaattctc taaactttct cattgtattt atgtcataaa gtgcttgtgt
    36241 ttggaaaaat ttagcccctg cttttatttt ctttttcatt tttaataatt gaacttctat
    36301 tggagaatat tctggagtta ctgatgctcc tagataaaaa tctggagaac cttttaactt
    36361 attaccagcc atgtccgtac ctgccattaa agtctcagca gtttgaagta tccccacaga
    36421 atctaaatca aacactccct tagcttgtgg atggtctcct acgctagtat ggtctcctgt
    36481 tagtgccaac agattgttta tcccaaatac acctgctgac aacatttctc cttgtatagc
    36541 tattctattt ctatctctcc ctgttatttg aataactggc tctaatccag catcttttaa
    36601 taatttacat gtagcaagag aagttgctct cataactgcc gattgaaagt ctgttacatt
    36661 tgctgcatga actcttccaa ctaacggctt tgcacattct attaaatgtg aaaggtcagt
    36721 cccttttgga ggagccattt cagtagtaac tgcaaatttt ccactttcta atgtttctct
    36781 taataagctc atctctaatc ccccttttaa ccttctaaaa aaaactttta tgaatttgct
    36841 gctgcttctt tctttttctt tgcacttaaa cttcttggat tattttgctt agaatagtct
    36901 tttggtggtc ttatttctgc taagttatct aattgtccta tagcttctag tctttcatat
    36961 atctttatcc aagcacaatc attctctgag tttacttcac acttaccatt tttagctcca
    37021 ccacaagccc cattcataag acctttagca cacatagtta ctggacatat cccacctgtc
    37081 caaccaagtt cacagtctcc acaagcctta caggcttctt catattctcc aactctttgt
    37141 acttccccta taaacaaagt attgttggct ggataaactg gcttatcttt taaattctta
    37201 actattgtct gtgtaccatc tccacatgct aaagataaaa ctgcatcagc ttccttagtc
    37261 tcttctttta gagcctttaa atccttttta gactttaaaa ggttacaagc agggtcaagc
    37321 attacatatc ctaagatttt tttgccttca ccttcaagag tttccttcat ttttaaaact
    37381 tcttcttccc caccactttt acaagttgct gcacattgat tacaaccaac taaaaccagc
    37441 ttgtcaaaat cttttaagta ccctagcact tcttctaatg gtttattttc agaaattatc
    37501 atatctactt cccccctaaa ccttccacat atatatatct atttattgtt ttgtaattta
    37561 caagccttaa ctacatgttt tgcaagtata gatgttgtaa ctgaaccaac ccccgctgga
    37621 actggagtta tcattgatac tttgtctaaa actgcatttg tatcaacatc tccacataaa
    37681 tttccttctt catcaacatt tataccaacg tctataacta ccgctccatc tttaacaaaa
    37741 ctttcatcaa ccatcttagc tctaccaata gctgctatta gcacgtctgc ctctgctgct
    37801 acacctgata gatttttagt ttttgaatga catattgtaa cagttgcatg ttcacttaat
    37861 aaaagcattg atactggctt tccaactacc attgatcttc caagaactgt taccttactt
    37921 ccttttaaat caacattata gtgttttaat atctcaacta ctgctgtagg tgtacatggt
    37981 ggaaatccac ttttatcccc ttccattacc ttagcagagt ttattggact aaaacaatct
    38041 acgtcttttt ccggtgcaat aacatatttt ataacttctt catttaagtg ttttggaaga
    38101 ggtctaaaac ataatatacc attaacattt ttgtcatcat taactctctt taatacatca
    38161 atatattcct cttgtgttat atcctcagct aactctaaca cttctgttgt tatacctatg
    38221 ttttgacatc tctttagtgc tcctctttca taagataaat catcacttct tgccccaact
    38281 ctaactattg ttagtttagg atttatacct tctttaatta gtaaatcgac ttcttttatt
    38341 aattcttcac taatcttatc agctacaggt tttcctttta taatttgtcc tttagttgac
    38401 attccttcca tacattcaca actcccctac aagcaaactt aaattaattt attctatata
    38461 ccacatatta ataacatagt atctactttc gtcatttaaa aattattttc caagagcttt
    38521 ttcaactttg gcataaactt catcacaaat ttttacacca tcttcaacta acttatctac
    38581 ttctgttttt accttattaa catattcttt atcttgtata gaatttatat ttataactac
    38641 atttaattgt ccaccaagta tagcagctct taaacactga actcctacac caacatcact
    38701 tatagcaagt cttgaacctt tatcaactaa atcttcatgt aacttaatag cttcatagca
    38761 aactcttact atgtttaatg gtacttcaca agcaactttt aatgcctttt ccattgtttc
    38821 tgcttttatt cttttttctt cttctgtttc tttaggaagc ccgtaagctt ttgataatgg
    38881 taagaaacat tctgcatcat catctatcat ttttaataaa tctttttcta actttcctgc
    38941 cttacttaat atctctttta cactttcttc atactcagca tacttcttct tccctattgt
    39001 aaggttacat accatactac ctaaagccat acctattgca cctactagtg cagcagctcc
    39061 cccaccacct ggtactgctt ctttggaaga tagtacttct acaaaatcaa cacaagtttt
    39121 atctgctatt ttcatgataa tcctccttga aaggatgtag gaaaatatct caaattgttc
    39181 gagatatttt acgaactatt ttaagatatc tcctactctt tatttataaa ttaatttcct
    39241 tagaataatc cagctattac tccattttca tcaacatcta ttttttctgc tgctggaact
    39301 tttggtaatc ctggcatctt cattatttct ccagtaagtg caactataaa tccagctcct
    39361 gctgaaattg ttgcttgtct tacagttatt ctaaatcctg ttggtctacc taatttagtt
    39421 tggtcatcag ttaaagaata ttgagtctta gccatacata ctggaacttt agtaaatcca
    39481 agtttttcta agttagctat ttccttatca gcttcaggag tataatctac accatctgct
    39541 ccataaatct tagtagctat agcatttatc ttgtctttta tagataaatc atcttcatag
    39601 cagaatctaa agttattttc ttcttcatcc aataatctta aaacttcttt agctactgca
    39661 attccacctt ctccaccttt tgcccatact tcagaaagag caacattaac acctaattcc
    39721 ttacacttgt cttcaacaag tttaagttca gcttcagtat caagtgggaa tctgttgatt
    39781 gcaacaactg ctggtaatcc atatacttga gttatgtttt caacatgttt taataagttt
    39841 ggaagtcctt tttcaagagc ttctaggttt tcgttattta agtctgcttt agcaactcca
    39901 ccattgtatt ttaatgctct tacagtagca actattataa cagcatctgg ttttaagttt
    39961 gccattctac attttatatc taagaatttt tctgctccaa ggtcagcacc gaaaccagcc
    40021 tcagttatta cataatctgc aaagtgcata gccattcttg tagctataac agagttacat
    40081 ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg atggtgttcc ttcaagagtt
    40141 tgaactaggt ttggttttaa agcatctttt aataatgcag ccatagcacc attagctttt
    40201 aattgtctag ctgttacagg ttcaccttca tagctgtatc caactattat gtttcctaat
    40261 ctttctttta aatcagatat atcacttgcc aaacagaatg ctgccattat ttcagaagca
    40321 acagttatat cgaatccatc ttgtctaact gctccatcac cttttttacc cataccatca
    40381 acgatatttc taagttgtct atcgttcatg tctacacatc ttctccaagt tattttctta
    40441 gggtctattc taagtgcatt tccttggtga atatggttat caagcatagc agctaaaaga
    40501 ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt gtaagtttat atcttccata
    40561 ggaacaactt gtgcatatcc tccaccagct gctccacctt taacaccaaa tactggtcct
    40621 agagatggtt ctcttaaagc aactaataca tttttgtcta gtttagcaaa tgcatcagca
    40681 acacctatag tagtagtagt ttttccttca cctgctggag ttgggtttat agctgtagtt
    40741 agtattaact tagctttttt accagtctct ctttttagta gattgtagtc aacttttgct
    40801 ttgtatttac cataaagttc tacatcatct tcacctagtc ctaatttttt tgcaacctct
    40861 ctaatatctt gaggcttagc ttcttgagct atttcaatgt cagatttaaa tcccataact
    40921 gaatctcctc ccacaaccta aatagattta aaatataatt tttgtaaaaa ttgtattaat
    40981 atgtaaaaat tatattttat aagataatta tacatgtaaa attacttaaa aactagctac
    41041 taagctatta aaatattcaa acttaatagc tagttattta tctaattact tacttattac
    41101 ctatctattg cctatgtcta acctttaacc aacttaaaat cccaaatcaa aatttctatc
    41161 tattttattt atccttaagt gacattaatt tatcttttat tgctttaatc tgttctttcg
    41221 ttgtttcact agaatcatat ggagattgat tagctctatc agaatcaata acatcttggt
    41281 tataataaat aaatcctaaa gattctccat cttctaagtt ttggattata aattcttcat
    41341 cttccttatt tctaatttta tttcctacta caaatacttt atttacacct atatcatgtc
    41401 caagtttctt aacttttcta taagtctgta gacttctctc tcctggttct acaactacta
    41461 taaaagcatc tacaccttgg gctgttcctc ttcctaagtg ttcaattccc gcttccatat
    41521 ccataaccac tacatcttta ttttgtagta ttaaatgtga gcagagtctc ttcaaaagaa
    41581 catgctctgg acatacacat ccagtaccac cagaatctac ggttcccaat gtaagtaatc
    41641 ttactccatt gtattcttta cagaaatttt cagggatatc gtctacttta ggattcatct
    41701 taaacatttt tccaaatgaa cctacagaag cagctgttct atctgaaact aattttttca
    41761 tttctgatat aggtactatt gattcatata cttcttttgg aaaacctagt gctaaagcta
    41821 aattagcatc agggtcggca tcaacagcaa caactctgta tccgtcttca gcaaacatcc
    41881 tagagagcat tgaagaaaaa gtcgttttac ctactccacc tttacctgtt attgctatct
    41941 tcataatccc taccaccttt tattactatt tgctttttag tagttaattt tacagttaaa
    42001 tattttttat actctatatt tttatacact aaaaataaac tacagctagt ccttacagga
    42061 accaattaaa tttaccctac tagcttttca aagcagcatg tatagtttta ttattcttta
    42121 atattgttta tattttattt attcagtata ttcattatat tttgatgttc tttactttaa
    42181 tatttgactt tattcaactt tattcaacca gaaacttctt ataaaaacgt ttattctata
    42241 acttcactat tttttaagct tcagcctttt ctgccatttt ttcttcaact aatgcttcaa
    42301 agtgaacacg ttttttctct atatcttcta ttattgtagc tgctaactct tttggatttg
    42361 tatttacagt aaatgtagct ccaactttat cttttaattc actagttaat atatccactg
    42421 ctcttgaaga accagctatt ggaggcatta ttcctaaata agtgtctata ccagaagcta
    42481 caacatagca tcctatagca actgcttttt ctgacatcca ctctggagct atacctacaa
    42541 ctggaaggtc acacatatcc atatctaagt aattagctgc tgctccaact aaatctagta
    42601 tacgacttat atcaacacaa gaacccatat gtagtactgg tgggattcct actaatttac
    42661 agacagttgc aagaccttta cctgcatatt tttcagccgc atcagattcc attaaaccaa
    42721 acttagctgc tgctgaagct ccacaacctg tagtaactac tattatgtcg tttttgatta
    42781 attctttcat tattgttacg tgagcctcat ttgaaactcc tttagcattg ttacatccaa
    42841 ctacaccagc tgctcctctt aatactccag attttagaca gtctgttaat ggcttaactg
    42901 ttcctgctgg gtctatatga gagtttacaa ctctgtctaa ttgatttact actgcttcta
    42961 cactataccc aactgttgcg cctgatttta attcaggtat aagtactttt gatttatctc
    43021 tatttttaaa gtttaaaata gcttctttaa ctattgcttt agcatcatca agtgcttgtt
    43081 cttctctaaa ttcaatataa gttgaacctg ttatcttagc ttttggagaa gttgttacaa
    43141 atttagtatg gtaacaatct gcaactctag ctaatgcagg gaatatacat tgaacgtcaa
    43201 ctattacagc ttcaactgct ccagttatta ttgcaagttc ttgttgatgg aagtctcctg
    43261 ctatttttac accatgtctc attgtaatct cattacctgt acaacacata cctgctaagt
    43321 ttattccatc agcaccaact tctttagcta aagctactaa gtctggctct tctgatgcta
    43381 aaactatcat ttctgataat gctggctcat gtccatgtaa tattatgtta actttatttt
    43441 cttctaatac tgctaaattc gcctctgttc ttcttggcac tggagttcca aataagatat
    43501 cgcttagacg agtacctatc atagaaccac cccaaccatc agccatagaa gttctaagac
    43561 tcatgtgtat taaactatct atatcaccag tacatcctat atgagtagag tgcattattg
    43621 ttgcaatttc tctatctata gctcttggct ctatagcgta ttctttccat attttttgtc
    43681 ttggttctgg tgcatttttt ataaatctta atgtcccaaa aggttttcca aattccatta
    43741 atgcaatttc tgccacttca tgtgctacat catagatatc tctaccttct gtctctacat
    43801 cccattcttt agctaaagta attaacttag cttcgtctct tattttatag tttccatttg
    43861 gatcagctaa tgctaatgta tgtgctatat ctctagcatg gtctgaatgg gcagctgtcc
    43921 caccagcaac cattcttgca aagtttctac ctactatagt atgctcatct gctccacata
    43981 acccccttgg agtcttaggg cttattctac atggtcccat tccacatatt ctacagcaaa
    44041 caccttgaag accaaaacca cattgtgcct taaagccttc ttttctatca tacattgttt
    44101 ctacgccttc ttctctagct ttagctatca tcttctggct gtttaaatca attgttaaca
    44161 ttttttcatc catattccta tccccttttt ttgattttta aaatccccca ggtgactgac
    44221 ttcatgtcat aaaataataa aataaatgct ttctaagttg attatatact aattttcagt
    44281 gctttgcttt agatttaaat atttttttaa caaaattaga taatacataa aaattttatt
    44341 ttctacactt aatttaagtt gattttatcg cttgtttttg gctatttgtt aatatctaaa
    44401 cctacttttt tgatacttca gtttaattaa attatgtttt gattttttta aatcaaattt
    44461 gaaatttttc tagtcatttt tatattattg cagattgttt gtgtatttat tatctttcac
    44521 tactgtttgg acttattaca gatttttact ccctaaactt aattagtaaa aacttcttag
    44581 aatagcattt tttggactaa tttttttgaa tttttttatt tttcctactt taatattaat
    44641 tatagcttca aattgaacat ttgtatatat tcatttgaag ctataattat tttttatata
    44701 aaactttttt aatacagcat taatttagac attttgacta ttttttctac tttaattcat
    44761 ttatttcaag cgatttatcc tatttcatat ttttagctac aatagcccca tatgcaatag
    44821 aatataattt agattcatgt gaatcggctc ttgacactaa aactatagga gcttttgcac
    44881 ccataattat acctgctgat ttagagtctg ccatatatgt aaaggctttc cctattccat
    44941 taccaacctc tattgttgga accaatatga tatctacatc tccagatact tcacttttaa
    45001 acccttttat ctcactagct tcttttgaaa cagctaaatc aaaagccaga ggaccttcta
    45061 caattacatt ttcaccaaat tctccttctt tacaagcttt tgctaacata tctgcatcta
    45121 cagttgcttg catcttagga tttactttct ctttagctgc caaacatgca acttttacag
    45181 tttccattcc taaagctttt gcagctttta tagaattttt taatatcttt gccttttgtt
    45241 cataatcagg agctatattc atacctccat ctgtagttat aagtaattta tggtattttt
    45301 ctaactcata taccataaca tgacttaata aactatcagt tctaagacca tagtctttat
    45361 ttaacacttc ttttaatatg acagatgtat ctaataatcc tttcattaca aagtcggctt
    45421 ctttacttga tactaattta actgctattt ctgcacactc tttgatatca tccttatcca
    45481 ctattttgat accacttaaa tcaaatccta tttcttttga tatttctctt attttgcctt
    45541 catttccaac caatatagga gtaattatat ttctttctac agcatccttt attgccatta
    45601 aaacttcttt atcatgagct gctgctacag agagtacaat tttctctcct cccttaagtt
    45661 gttctaaaat atcatctaat ttttttatca aaagtaaccc ctcccttata tatatttaac
    45721 taaataatac catatttgct ataccttttc tcaatttatt ttttatgtta taaagaattt
    45781 atataactaa aagacagttt aatattttat tctgggattt catcaataac tcttaaaagc
    45841 tttgcaggaa caccacctac aatagtattt tctgcaacat tttttgttac aactgctcca
    45901 gcagcgataa ttgcattatc tccaatagta actcctggaa ctacagttac attagcacca
    45961 atccatacat tttttcctat tgtgattgga gaaggataaa ttgtaccacg tttctctggt
    46021 gcaataccat ggttaagtgt agccaatacc acattatgcc caatttgcgt attatctcca
    46081 atctcaattc ccccctggtc ttgaaaatgg caactgctat ttataaaaac attttttcct
    46141 attgtgatgt tttttccaca atctgtataa aatggtggaa acataaaaaa tgattcatct
    46201 accttttttc caattaactc agaaaataaa ctacggactt cttctggttc atgataagaa
    46261 gaatttaact tacttgtgat tttcattgct tcattactca acttaaccat aaattcatgt
    46321 atttctgatt tactagaaac aggttttctt tgattgatat gtgttaaaaa ttcattgata
    46381 ttcattttat acatcctttc aatatgtgct ttaatttatt tgtacattct aaataatatt
    46441 ttaataaaaa ttaatttaat ttatttttat taaaatatca ttgcttcttt tagtgtaata
    46501 tacaattata tctatatcaa atatctatat taaatactta actatactta aaaagcatat
    46561 aaattgatat aattatataa tagataagtt aatattgagg agggataatt atggaaataa
    46621 gagtattaag atatttttta gcaattgcaa gagaagaaag tatatctggt gcggcaacct
    46681 ttttacatat aacacaacca actctttcta gacaaattat ggaacttgaa gaagagttgg
    46741 gagttaaatt attggttcga ggaaatagaa aaatcacctt aacaaatgaa ggtaggcttt
    46801 tacataaaag agcagaagaa attatatcat tagttgataa ggttgaatta gaatttgaca
    46861 atcccaatga gattattagt ggtgatatat atataggaag tggtgaaact gaagctatgc
    46921 gacttatagc tagaactgcc catcattttc atcaaaaata ccccaatgta agatatcacc
    46981 tttttagtgg gaatgctgat gatgtgactg aaaaattaga caagggactg cttgattttg
    47041 gtattttaat tgaacctgca aatatacaga agtatgatta tgcacaaatt cctgctactg
    47101 atgtatgggg tttactaatg cgtaaagata gtgcccttgc atcacaaagc acaataaatc
    47161 caaaagattt atgcaatata cctcttatta tttcaagaca gacacttgtt gataaagcaa
    47221 tttctaaatg gcttcaagac agtaatgaaa aactaaacat tattgcaaca tacaatttag
    47281 tttataacgc ttctcttttg gtagaagaag gtctgggcta cgctctgtgt ttagatagac
    47341 tcgtcaatac tacagaaaac agtaatcttt gttttagacc tctatctcct cctttagaag
    47401 ctaaattaaa tattatatgg aaaaaacatc aaatattttc aaaggcagca gaaaaatttt
    47461 tacttcaaat acaaaaagat ttttcttcta tcatgttgga tacatctaag ttggatacac
    47521 ctaaagaaaa ataaatcata ataaaaagtg agagtgtctc aaaatgaact ttgcactgta
    47581 taaggcactc ttttttatac aaagtaaaat atatgttcat catttcagca ctaaaaaaga
    47641 ggcttatctc tattttgaga tagccccact taaccaataa aattaaatac aacttaacaa
    47701 tatccttact tatttattcc aagttctctt agacaagcaa tagtaatttc atcttgtgga
    47761 tttaattcat aagctctttc tatatagtaa gttgcatcat caatatcacc attatttaaa
    47821 taagccattc ctaaattgca aagtatttct gggtcttcct ttatcttagc cgcttgttca
    47881 aaatactcta ttgctttatc taaattattc ttataagcct cacaaagccc caattccact
    47941 atagtatcca cctgattagg ctttattatc aatatcttct caagatacat cttagcctgc
    48001 tctatttcgc ccataccctt atatccaagt gctatcataa acagcaagtt ccaccaatcc
    48061 atatgttctt cttctaatgg aagtaacttc tctaatcctt ccttaaactt accttggaat
    48121 acaagtgtat atccttcttc atattgaact ttaaagtcca tcttgcccag attctcttgt
    48181 acctcagcta ctaaatcagc atctagccct agccttaacg cttcttccca agtcagctta
    48241 gtctttaaat attgaccttg attataataa tggtagccta agtgataata acctaatgca
    48301 aaattctcat caacatcaat taccttttca agcttagcca tggcttcaag taagaatgca
    48361 ttcattgcct tatcatccat atctttttga tactgtttag ctatctcttg gcaaacaatc
    48421 gcatagttat acattgcatc taaatcatct ggatacattg ttataaatgc ttttatatat
    48481 attaaagaat ctgtatgttc accaatctca aagtattttc tcgacatata tcccatatac
    48541 gatttcaaat ccaaatcaat atcttttgat aaagcattta aaaacttctc atactctgca
    48601 ttgtttttaa acttactatc tattccaatt atatatatca ttgcatctgc tatagacatt
    48661 gaatttaact tatcttgagc agtcttttct tttatccctt ttactaatac ctcatttttt
    48721 ataggtacat ctaatccacc ttttggcact tcatagcttt ttaatttaaa ctctccacca
    48781 tgttttatag ttataaaagc taattcttct gcttttttta gcaaatattt ttctatctta
    48841 aaatccattt taccctccta aatacttaaa tctctacttt atgagtttta gatttctcaa
    48901 atattttttt atcatcatac tcaaataaga ctacattttc tacttctctt atttctgtat
    48961 ttatatcatt ttttaaatcc acaacattaa atctaaatgg ctcaaaatga acatatttta
    49021 ttatatgttt agctggttta tcaacaaaac taggtaaata tgttgataag ttatcattat
    49081 cttgtaaaaa tacatggcat ctattcttaa aatcatccac atctagctta ttaaaaaact
    49141 gtggtatatt tgatgtttta cccagtgata tatagtcata ttttaatata tcgttgaata
    49201 catcattatc tatgttgatt ttttcattat agaaatccat caatatttta tataactgat
    49261 tctttccttg tgccaaatca aagtagccat tttcatcaaa atacttggca aaaacctcaa
    49321 agaatttaaa agggctttgt ttataaaatc tgcctataat atatctcata gataacacaa
    49381 agtttttaga attgtagtat ctctccagta tatcttctat atcctttagc ttcaaagttt
    49441 cttcatagct tatataatta ttgtataaaa actcatatgg agcataatcc ttatatttaa
    49501 aaccatgctc atctgcaact tttctcatac ctgtcccttt aatcatcttt aaaaagccta
    49561 attgcagatg ctctatacct agattaaata catcattaaa agaattttca aagctcttat
    49621 aatcttcata aggcagtcct gcaattaagt ctaagtgctg atgtatattt ctataagatg
    49681 ctacagtttg tacaacatga gacaactttt taaaatcatc ccttctacct actgcatcaa
    49741 gtgttttttc attcgttgac tgaaccccta tttcaaattg aaaaagacct tctttacaat
    49801 cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc tgtaacttca aaatgataag
    49861 ttgtatatcc attatcattt ttcataagga aatccatgat ttccatagcc acttttctat
    49921 ttgcattgaa tgtcctatct ataaacttta tctgtgatac tcttgcatct attaaagctt
    49981 ttaaatcact ctttatcctg tctaaactaa aataccttag acctggaata gtagatgata
    50041 aacagtactg gcagttaaaa gggcaacctc ttgatgattc ataatacact attctatttt
    50101 catattcttt tggattcaag ttttcataag gactaggaat ctcatccaaa tcttgaagta
    50161 attccatagg cttattcact attatctcat tgccttgtct atatacaagt ccatttacat
    50221 catttatttc catctttcct tgcaagtgaa gtactaagtc tctaaaaatt aattctccct
    50281 ctccataaag tatataatcc acaaaatcat actttttcat tgcctcataa ctatcataac
    50341 tcacttccgg tccgcccaaa gctattttag tattaggtct aatttttttt agattatcac
    50401 aaagtttaac tatatctccc acattccaga tatatgttga gaataatatt atatcatatt
    50461 catttttata tatatccttt aaaatatagt ccaaatcgtt gtttatcgtg tattctttca
    50521 tattaacttc tatcaaatct cttacaaatt cttttagata tcttatagcc aaatttgtat
    50581 gaataaactt ggaatttaat gttgttaata aaattttcaa atatttctca cctcttttta
    50641 agattatact gtctttttgt accccttata ggttaaaata gtatatgtaa agtctaaata
    50701 atatctatat aattaattta ttaatttcaa gtaatgtgag gaaaggaagt tttttatgga
    50761 attaattgaa aaagtatcta tcaactcttt atcaaaaaga gatttactac ttattataaa
    50821 gtctcttgaa tacactaatg aaaatactca aattagtgat ttcattgacc ttaggaataa
    50881 tatagtaaag gaattatgtt ttttaactga cactaaggaa aaagattttt tagattatct
    50941 agaaaaaaat agcacactct aataacctaa acctttttaa actttaatat ataaatacct
    51001 gatttagaat ttatttttat gcttaatata ttatcattaa aggttttcga cataactgtt
    51061 atttgattaa atatagggtc tattatagta ttaccttctt caagtcttat aagacttctt
    51121 gggtctatgc ttttattata tgccttaact ccaattttat attttctcaa tttaacaaca
    51181 tattcggaca tagaaaaaat aaaatctata ttatcttcat ttaatttact atcttcaccc
    51241 aaaatggata atatagattt ttttatgcta ttaattcctt cattttctac agttctatct
    51301 tccatatata catcatatag aacatttaaa acatttaata aaatactttc ttcatcataa
    51361 tcttcagatt tcaaatcaat gtacttatca attaattgta ttgctttaat ccttgccatc
    51421 tgaaacttta caacatctga tttgtctaat tctatagaaa atcctattat cttgtcttta
    51481 atactctgta gtaattcgac atactctatt ttactattat ttataagatt gtgcataatg
    51541 gaattataca ccacagaacc taataaataa tcaaataaaa gttcttcttt tttaaaataa
    51601 ataacatact tttttattag tttaataacc tcatcttcac ttttttgaat attagtattt
    51661 gataaaatta gtggtaataa aatatttaaa aaattgatat ttcctatttt ttctccataa
    51721 aatctatagt aaataagttc atctctcagt ctattttcgt ctatatacac acatctttgc
    51781 gttttatcat ctattaagga ttttgtattt attatagtta aatctttaag agccctactt
    51841 gcactactaa aagaagatgt gtagactttt tttagtaatt cgtcattcat aaatatatcc
    51901 tttctgaaaa attgaatgta aataaattgt tattaaaaaa aaagagacag cccgtttaag
    51961 aagctgtcat atataggggg atagtagtgt acaaaaatca ttgtaatcta ctatctatat
    52021 attattatac gatactactt ttttatatgt aaagcatcag ttttcgacat ccctatattt
    52081 tacagttata caaattgcgt actaagttca attttattct ttttatttca acattttatc
    52141 atgctacatt ctttcaggag cgtgcattcc caacaatttc agagaatttt ctaaagtttg
    52201 ttttgttgct aaaactaatg caagtcttgc ttttcttatt tccaaatttt ctactaaaat
    52261 tggattgtca tgataaaatt tattaaacgc ttgagctaaa tctaacataa atctagtcac
    52321 tatatgtggc tcatttttct ttagagcaag taaaatattc ttattgaatg attctataac
    52381 tctaagaact tctgcactat cttcatcagc taatagacta taatctatat cagaagtcac
    52441 ttcttcttct gcttttctta aaactgcaca gcatctagca tgtgtatact gaacatatgg
    52501 tccagtctca ccatcaaaac taagcgttct ttcccaagag aaggtatagt ctttaattct
    52561 actattagat aactcttgga atactactgc cccaacacct acttgcttag atatttcatc
    52621 cacattttta gcatttgggt ttttagctag tataatttcc ttagttttat ctatagcttg
    52681 atttaaagca tcttctaaaa atactactct tccttttcta gtcgacatag taccttcttc
    52741 aagtgcaacc ataccaaatc caacatgaat taaatctttt gcccaatcat atcccataag
    52801 ctcaattacc ttaaaccatt gttggaaatg taagttttgt tgagaaccaa ctacatatat
    52861 acacttgtca aaatcataag tttcttttct atatattgca gcagctaaat ctcttgtcat
    52921 atataacgtt gaaccatcat tcttagtaat aagtgctggt ggcattttat actcttctaa
    52981 atcaacaatt cttgctcctt tagactcttt taaaagattt ttctcttcta atagttcgat
    53041 aaccctgttc attttatcag aataaaagct ctctcctgct aaagaatcaa attctatatc
    53101 taaaagttta taaactctgt taaactcttt taaactctca tttctaaacc attgccataa
    53161 ttcttgtgct tcttcgtcac cattttctaa tttattaaac catgctcttg cttcatcttc
    53221 catttctggg tgttgttcag cttcatcatg gaatcttacg tataatttta gcaattctgg
    53281 aattggatta gactctacta cttctttttc tccccacttt ttaaaggcca ctattaactt
    53341 accaaactga gttccatagt cacctaaatg atttattctt atagtcttgt atccttgcga
    53401 atcatatatc ttatataaag cattacctat aactgtagtt cttatgtgcc ctatatggaa
    53461 aggttttgct atatttggag aagaatattc aactattaca gttttatctt ttccaaaatc
    53521 actatgtccg taattttctt tttcatctaa aactttttta attacagttt ctgctaattg
    53581 agatttattt acaaagaaat ttacatatcc gcctaattgt attacttttg ttatctctcc
    53641 acttggttct atactttccg ccaattcact tgctatcatg tttggagctt ttctaaatac
    53701 tttggctaat ttaaagcatg gaaaagcata gtcccccata tccttatttg gtggaacttc
    53761 tataagtgcc tctatctctt ctttcgataa atcttctatt ttttctttaa ggcaatttga
    53821 tattgccacc ttaaaatctt gcattctgtt gctcctctct acattgttcc tggtttatat
    53881 tgtgcttatc tctttatgtt aaatcaaatt tcgtgttttt tcaagtattt cattacagtg
    53941 taaaacactt taataattat gttttctgta tatattaaaa atactataat tgcagttttt
    54001 aatcacacta ttattgattt tttataagac ttttttctat tttattgttt cttgtactat
    54061 ccagttgact ttatatactt tttttaatct tttatctatt ttcatacatc aatgtcagct
    54121 ttatatactt tttttactct tttatctatt ttctataaat ctttttcact cttaactttt
    54181 attccttctt cttctaatag ccttgctgtt atacctttcc ctggtatctt tttacctgaa
    54241 aaactaccat catatattag attacatcca caagaggggc taccctcttt tagtattgct
    54301 tcgctacata catataaatt tgctattttt agagtttcat tagcccctct tacaaagttt
    54361 tctgttacat ccttattatc tttagtaatt acttttccat caactatctc agcaggtggt
    54421 cttggtgtag atagaccacc taactgctct gggcatacaa gtgtaaattc tttgcctttt
    54481 aaatattcct tgactttttc attatcatta ttatcgccac tatatttaca atttattcct
    54541 attaaacatg cagataccaa tatcataaaa atgtgctcct atacaaattc ttaatatatt
    54601 ctcatacaaa ttactaatgt attcttatac aaattatttt aatttaacaa ttgttactcc
    54661 tgctccacct tctccatatt gaccacctct ttgtgatttt acatgtctat ttcttcttaa
    54721 aatgtcttgt agccctgctt ttaatactcc tgtacctatt ccatgtatta ctgttacaga
    54781 ctctaaacct gccacatatg catcatcaag atacttttct acttccatta tagcttcttc
    54841 taggtttaac cctctaaggt caacttcatt ttttacacta ccagattttg accttataat
    54901 gtttctcgtt gatttagtta catttgttga aacatctttg cgtgtttttt gtaaagattt
    54961 aaaaggtaga gtcatcttca ttataccaat ttgcactact gcttcttttc tctttttatc
    55021 tattgaaact acactaccat tttgatttaa agttattacc ttaacttctt cacctggctt
    55081 taagtctttt atctccttat ttgatacctt tggaactatc atacttttta ctgttggctg
    55141 aagacttccc atagagctag tcaattcttt tctaagttct tctatcttac gattcttttc
    55201 ttttgaagct ctttcttgtt caagacttct aagttctttt attataatat ctacttcttc
    55261 tttagcttgt cttataattg aaaatgcttc tgatttagct ttttcaatca tcttatctct
    55321 ttgactgacc aatttttcaa gtttttcgtc atactctact ttcagttttt caatctcttc
    55381 ttttaatctt tcagcttctt ctctatcttc tactgctttg attctattct tttcaacatt
    55441 ctgtaacaca tcttctaaag ctatgttttc tgtatttata tattctttag cacgtgaaat
    55501 cacataatca cttagcccaa gttttctaga aatctcaaac gcatttgatt ttccgggaac
    55561 acctataagt aacttatatg ttgggcttaa agtttctata tcaaattcaa ctgctgcatt
    55621 ttctacacct gatttagtaa gagcataatt tttaagctca ctatagtgag tggtagctat
    55681 acatttagct cctacagaat ttatatcttc taatactgct attgcaaggg cagctccttc
    55741 aacagggtct gttcccgctc ctaactcgtc aaatataact aaagagtctg ctgttacgtt
    55801 ttgtaatata gatactatat tcgtcatatg agatgaaaat gttgatagac tctgttcaat
    55861 actctgctca tctcctatat ctgcaaatac attatcatat acacacatac tacttccata
    55921 atcagcaggt atatgaagtc cactttgtgt cataagtgca aatagaccta cagtttttat
    55981 tgtaacagtc ttaccacctg tgtttggacc tgttattacc aatgtgtgga agtctcttcc
    56041 taaataaatt gtatttgcta ctaccttctt tttatccaac agtggatgtc ttccattttt
    56101 tatgtttaaa tatttatcct catttaaagt tggttctatt cctctcatct gtatagaaag
    56161 ttttcccttt gaaaatgcaa agtccaatct tccaagtatc tcttgattag agattaaatc
    56221 ttcgctcact tctccaacca tagcagaaag ctctgataaa attctttcta tttcctcttg
    56281 ctctcctagt ttcaactgcc tcaattcatt gttcatctct actatagtca ttggctctat
    56341 aaaaagtgtc gcaccagaag atgactggtc atgcactata ccagctactt gcgacctata
    56401 ttcagctttt acaggtacta caaatctatc acctcttagg gaaattattg catcttgtaa
    56461 gtacttttga taagtagttg aacttattat tgaatttaat tttgacctaa tggattgatt
    56521 tttttgggct attcttcttc tgatatctct aagtgtactt gaagcattgt cagatatttc
    56581 tacttcacta attatagcat tgtatatctg gtcttctatg tccttgtata catacagaga
    56641 atttgataga gattgtatta ttggataatt aaaatcttct tcatcactac tagataagct
    56701 atttcttaat cttctagcca ctcttaaggt atctgctatc ataatcagac ttcctgggtc
    56761 aagagaagca cctatataag ctctctttac cttatcttct atatcatgaa taccttccaa
    56821 ccctactgaa cctctcttta taataattga ttgagcttca ctagtttctt ctagcataga
    56881 tttcacttct acaaaatcag tatttggtac taaattttct atatatttta gacctaaaga
    56941 agatgaggct ttcttcttta acaaatctat tattttattg tattctagaa ctcttaacga
    57001 tttctcattc attttctata ttcaccatcc tcgatatttt aactttttta tttaatttta
    57061 aaaatgtatg ttttcaagca tttccaataa aaactgcaag ttttatttct ttttcatttc
    57121 taaaattatt atattttttc tcttttagat atattttaca catattttgt attatactat
    57181 gtatgtagct atctatatat tatacataaa catgctttat ttgtaatata atctgttaaa
    57241 aaatcttctt taatattttt aaaggagttt attttagctt ataaaaaaaa atagctactt
    57301 atatagataa attaaggagg aacatgagtc atgggaaacg tattagaagg attaaaacca
    57361 gaaagtgttt ttaaaaactt tgagaaaata tctcaaattc ctagaggttc tggaaatgaa
    57421 aaaggcataa gtgatttttt acttagcttt ggtaaaaatt taggtcttga aactattcag
    57481 gatgaaagct taaacataat aataagaaag cctgctacaa aaggatatga aaattgtcca
    57541 ggtgtagttc ttcaaggaca tatggatatg gtttgcgaaa aagaaaagaa tgttgaacat
    57601 gattttctta aagaccctat aaaattaaga attgatggtg acatgatata tgctactgga
    57661 actactctag gagctgacaa tggtatagct gttgctatgg gtatggctat attagaagat
    57721 aatactttag agcatcctgc actagaagtt ttagttactg ttaatgaaga agatggtatg
    57781 aatggtgcag atgcactaga ccctagctta attaaaggtc aatatatcct aaatatggac
    57841 tccgaggagg aaggatactt attagttagc tgtgctggtg gtaaaacttg tgttgttagt
    57901 ttacctgttg aatacaagga agttaaaggt gacaaacaag gattattagt tgaggttact
    57961 ggactattag gtggacactc tggtatggaa attgttttac aaagagcaaa tgctaacaaa
    58021 gcaatagcta gagtattaag tgtattgaat gttgattatg aattagcttc tgttgatggt
    58081 ggtactaagc acaatgctat acctcgtgaa gctaaatgtg taatagctgt taataaagct
    58141 gatgttgaat ctgctaaaaa acaaataaat gacattttaa ctgcatttaa acatgagttt
    58201 actacttctg accctggcat gacttactca gtagctgaaa cttctgtaga caaagttctt
    58261 actaaagatt gtaaagaaaa agttgttcaa atgtcttgct taactcctca tggagttcaa
    58321 tcagtaagtc ttgatataga aggattagtt gaaagttcta ctaacttcgc tataattgaa
    58381 actaaagaat ctactattga atttttaact tctgttagaa gttctgttat gtctattaga
    58441 gatgaaatcg ctgatagaat aagacttttg gctcaagctt taggtgctaa ttacgacctt
    58501 atagctcaat acccagcttg ggaatttaaa aaaggctcaa aattagaaaa aatatgcagt
    58561 gaaacttatg agaaattaac tggtaaagtt cctactgtta tggctctaca tgctggtctt
    58621 gagtgtggat tattacttga taaacttcct catgctgaag ctatctctat aggaccagat
    58681 atgtttgatg ttcatactcc aaatgaacat gttagtatcc cttctgtagc taatgtttgg
    58741 gattatgtta ttgaaatatt aaaatctatg aaccaatatt aaatttaagt tttgattcta
    58801 agtagatttt aattttaagt aatctatata aaattaactc tgtaaattaa tactactgtt
    58861 agtataatat ttactttcaa tattaactta tagaaataaa gcctaatgat agataataac
    58921 atgtatcatt aggctttatt gtaatttaaa tattattaag ctttattctg ttttaaaatt
    58981 aattcttata ctattttaaa tctatttctt ccttgtctct tagaatcata aagagcatta
    59041 tctgctattt gatacagttc atcatactct tgaccaggct ttgctataac tacccccata
    59101 ctaatagata aactttctcc atttggcaac ttaatgagat gaatatgatt gtatacatca
    59161 ttacatttat taataagttc ttccttattt attatatcat tgatataaac actaaattca
    59221 tctccaccca aacgacctac tatatcattt tttgcaaaac tttcatcaag tacctttgca
    59281 acatcaataa gtactttatc tcctaacaag tgaccataag tatcattaat cgacttaaaa
    59341 taatcaatat ctataatcaa taaagcacca tttttttcag ttgataaatt caacaaattt
    59401 tgtgcagtta attttctaat atatgctgca ttataaatat tggttaaact atccctttga
    59461 gccttatcta acaatctatt cttttcctct ttttcagaat caatattaca tattttcccg
    59521 attacataca ttggtatttt tctatcatta taaattattt ttgaagtaac tctaatccaa
    59581 tgaaaagtat catcaagcat aatagaatac atttcagttg ttccattttc ttgtgatttt
    59641 attacatcaa aaaacttttt attatattct ttccttattt tatctctttc attttctata
    59701 ttgccatttt cactttcctt atcattttta tccaaaccat aataagtctc aatacccttt
    59761 tctggcatag aaattatcaa tttatcttgc ttataatcat actcaaaaaa gtattcactt
    59821 gtcatttcat acaactgagt atgttttttg agttccaaag atatcttctc gttcatactt
    59881 gtacgagtat gtaaaccaaa tgccataaat ccaataatac aaattaataa actggctaca
    59941 ataaaaatag tctcaattgg attctgttgc ataatataaa gcaacgagaa gtcttgtcta
    60001 tatgtagtat tttggtagac aattgattgc attttttctg atgaaatact tataatagct
    60061 ttattaaata tattcaataa ttcttgttta tcttttttaa ttataccaaa acaaatactc
    60121 tttgctttat aagtttgagg aactagacgc acatttttat acttaggttg attcatatag
    60181 tactgcatag aataaccttc gccataagtg taatctgcat agcctttatt aacagcttct
    60241 atacactctt ctaaattatc ataccaaact atattctctt caaactgacc ataataatta
    60301 atacctttgg ctagagctac acgttttcca tctaaactat tctcatttaa tttattatta
    60361 atcatcatag tatattgagt agaaagaaaa ggccgagaca taactatatt atgttcacga
    60421 gagtattcat aatcataaat cattcctgct acaatatcaa tttcacgctt tttagtcatg
    60481 ttttctaaat cttcttgaga ttttacaggc acaagttcaa attctaagcc tgttttatca
    60541 catatatgat ttaacaaatc aattgatata cctcttaatt cacctgtatc tttatctata
    60601 ttttgaaatg gtggtttgtt taaaagtact cctactttta atttttctgt ctctttaatg
    60661 taatcagttt cttcatcaga taaaaacatt tgattgttct ctggtgtaaa gtatttttca
    60721 tataaagtag ttgaaaaata tgggtctttc tgttcaatat tattaattgc tgagttaagc
    60781 ttttgagtta aatttgtact acctttagga gtaatgaaat aaaatggttt tgatgaaaat
    60841 tttgctacag tacgtacacc ttctagtggg ttcatactca cattaagaag tacatctgct
    60901 ttaccactct ttagtgcttc tagttgctca tcatttgttt tacaatatac caattcaggt
    60961 gacataagat tcattttaca atactcatct aactctttaa gtctagtatc agaattttct
    61021 attacagcaa tacggatttt tttcacatct tgtgaactca ttatgttatc aatatctgta
    61081 ttttcatata atgcttgtag tactgtattt gctactccat aattattccc tgcataatta
    61141 tatatttttt gcattggctc attgtataac atccctccca ttatatcaat ttcaccattt
    61201 tctaacatct tcattaattt tgtaatagat tcatttatct ctccaggaac ttgaacaaat
    61261 tcataattcc atccagtata ctgtgcaatt tctagtaaat attcatatgt atacccaaaa
    61321 tagttacctt gctcatctat ttcagttaaa cctttttgaa taggataagc aacacgaatg
    61381 gtacgattat cattacctat agcgttgata ccaaaagaaa atatgcaaga agtgaatata
    61441 gatgtaatta caataattaa taaaagtaat ttttttaaat tcactacaca atcctccata
    61501 tagttaagat ttacagtata aattactgca taaacttgta gacatgaaaa aatagattag
    61561 tatataaaag gatatataaa tctacttaga gatttaaata tccttaatat atacaatata
    61621 tttaaattat aactcattta tatgtctatt acaacaaatt taccctgcta aatacctact
    61681 ctatatccca taattttaca agttgtagtg aagtttaagt tctatctcac ctcaaattta
    61741 gcagtgtttc tatttacagt tcttttatat ttcacatctg gataacccaa tataaatgaa
    61801 gtgataacag cttcgttctc gctcattcct aacagttctt ttatctttgg ctctacattt
    61861 gatgccataa caaaaaatcc attataacaa attcctaatc cttgagaata ggccataagt
    61921 tccatattag atgcagccag tcctccatct acataagcac ttccacctag agacatatct
    61981 cctacaacta ccataagtag tggggcatca taaaataaag aatcatttcc gttctcttta
    62041 tatcttttgt acattttctt aaatgtattt ttatagatag aacgtacagg gtcattgtca
    62101 tcagtatcta atgccaaatt ataaagacct tgtattgcca gttctttcac ttcttttagc
    62161 ttttctttta ctaatatgta tcttataggc tgtctattac cacctgttgg tgtgtatcta
    62221 ccagcttcca ctatgttttt tattttttct tcttctacct ctatatcttt atattgtctt
    62281 atacttcttc taaatttaat aaaattcaat aacttttcgg gttctaattc aaaactttca
    62341 cgattgtatt ctattacttc atttttatca aatccttgca tttctattgc atctgttggg
    62401 caaattgcca tacagtgacc acagttaata cagagaatat ttttaacctt tgctttatca
    62461 tccttaactt ttattgcatt tacaagacag tctgattcac acatcccaca gcctacacat
    62521 ttttctaaat ttatgtctat cattccaaat tattcctcct tttaaaaaat ttactttagt
    62581 ataaaacaaa tagttgtcta agtcaatttt tattttttta acaatatact agccttaact
    62641 acactttaat ggagaaaaat atacataaat tggaacttaa attagaactt attcaaagta
    62701 aaagataaaa gaagtagatg tctaaaagtg aactttttag ttaattagat attctttatt
    62761 gtatgaaatc aagtggatat ttatcatttc aacaataaaa aaggcttacc tctattttgg
    62821 atatgtccct gtcaagtaga catactaaaa aaaggctatc tttatgcact gagatagccc
    62881 cttttcccaa ctactattgt ttatcttaat attagtcaat aaatttaatt tgctataacc
    62941 tgtaagttac attttaattt gatgtaactt taaattacat ttttaagtaa tatctttaaa
    63001 ttatattctt aaaatatatc attaaactac attttaatta atctaaaaac tatatcttaa
    63061 ttaatctaag aaacttacat tataatcttt tagtttccca ttatcatata caatctttgc
    63121 actaaagata tttccagtct tagcccaatt taagaacagc ctgtcacctt cccacatatt
    63181 taaatcgaat attttatcat tatcaaccca aattaaattt ccttctttac aatcatttaa
    63241 atctttttca gataattcac cttcaaactc atctgagaaa tacaaaaaca tataccaatc
    63301 ttcttcatct ttaaattttg gaaaagttat caacccttta tatgacaatg atttaagagt
    63361 aagaccagtc tcttctttta cttctctaat tacacattct tctggagtct ctccttgctc
    63421 aaactttcct ccaactccta catacttacc ttcatgtata tcatctttct ttttatttct
    63481 gtacaacatc aaagtcttgt catctttttc tatataacaa atagttgtaa gtttcatgta
    63541 tctcgccttg ctttctcttt atattctcaa ctaattattt tcagcacttt caagcacatc
    63601 acttatattc ttcattgcat taagagaaag ctctgcaaat tcatcaaatg gaatccctat
    63661 atcttctata cttctcattg catctctatt tacatttgaa gcaaatctcg tttctttcat
    63721 tcttttaata acagacttag tctttacact ggtaagtttt ttatctggat aaactaaagc
    63781 tatagcagtt ataaacccag ttatagggtc tgctgcatat attgcttttt ccattaggct
    63841 agttcttgta actcccattc catcatgatg accaagtatt gctctataca tttcttcgtc
    63901 accgaagttt tcttttttaa gcagttctac tgttgttgta gcatgtcctt cacatatttc
    63961 tcctggcttt cttccaacta tatctgaatc caaatcatgt aaaagacctg ccactgccca
    64021 ctcttcttct ttttctggct ctagtctttt ggctaattct ctcataacag cttcaacagc
    64081 ataacaatgt tttctcattc tatcagtttc taaatactta aataaaattt ctaaagcttc
    64141 ttctctcatt ttcaccaatc cccctgtttt ttcacatata tgatattttt atatattaat
    64201 acacataaat tcaagttttt atatatattc atgtatattt ttcatgttat atctattcta
    64261 cacctttata gtagtgtcct gcaactgttc caatttcatc taatttctta tacaagtctg
    64321 tagccttttt tccaagttta taattattac tcaatacctc aatgtgagct tcaataacct
    64381 ttttaaccat ctcactatct tcaactgtaa aatctatcct aaatacatct attccaattt
    64441 cctctaactc atatagattg tccaacatac aagtaacctt cgaattgtaa attgtacttc
    64501 tacagaatat atcttgtgtt atcctataac ttgcatccgt aaagtctctt aaagcataac
    64561 tactttcctt acatttagca actctcttgt cctttttaca atctctaaca actactccca
    64621 taggacagta ttctgtaacc atcataggag catatccata tattactgct tcaatttcta
    64681 catctgtata ttttaaagtt tgtttaattt catttaagtt tagttcttgt gacaaacaaa
    64741 ctgtacttgc tccctcttct ttgaagtgat ttatactttc actattgaat gcatttaagt
    64801 agcaatctat atttaagtct tttttcttaa agtattccat agagccccag ttaccaattt
    64861 gaaccttatt aattcctttt atattatcca atatctcaaa ttgcttgtac tctttatttc
    64921 taacaatcct tggtgctgaa tagattaatt ttttgtcagt gactgctgta ttatagtggt
    64981 tcttattcaa agtatctatt ttacttgtat ttgacaaagt atctatgtta tttgtatttg
    65041 aacccattag ttctttagga gtacctaaag acgaagaagt atctaaatcc atagaaatac
    65101 ttgaatctat agaaatattt gaatctatag aaatacttga gaccatttgt aaagcttttg
    65161 ataatgtatt tatatcttca taatagatta aatctacatt ataatttaat actgcttcta
    65221 attgctccaa gttcttaact ttaactctaa tcttaggagc tgtatcaagt tttttctgtt
    65281 tatttactct atttggctgg tattttaatc tattcttctt aaattttcta tcttttatct
    65341 ttattctttc ttcatctaaa agctcaatac aatcccttct catttgattt aacacactta
    65401 taggcatact tacaccttca tccaggtcaa tttctatctc ttttaactta tatggtgtat
    65461 ttccaagctt ttgaatttgt gtttctattt tttctttact taaagccacc tttatagctt
    65521 tttcaactat tttgtctcct tctaccaccg atttatttcc acgaaaatca cttataaaaa
    65581 gctctggctt tttacctagt ttaatactca cttttgcacg tatattagtt tttatgaact
    65641 ctttatcttg ctgaaaagta gcttgtactc tatccatcaa ttcgctgtca gaagtcttat
    65701 atataagctg atttctcttt gcttctccaa caaaatctaa ttctatcaaa tcccctttat
    65761 gtgctattgt agttatctct ccatccttta ttattcttcc aatagtacct ccacctaagt
    65821 taattccatc acctttctta agtgtatttt ctagcattac tctaagtctt ttgttctttc
    65881 tattataatc taaaactttt ccaatatata gcccttgatt attaggtcta ttagagttca
    65941 ttatatcctt accaacttca cctaatatat atccttttgt aaactttcta ttaaatatgg
    66001 tatacaaatt atttattatt tcctcagaaa tacttatttt tttcttatct acaaattcat
    66061 taatcgcttc tctatatcct gaaaccacag tagctacata ctctggcttt ttcattctac
    66121 cttcaatttt taaagataat acacctgctt caattatttt atccaaatct tctatagtac
    66181 ttaaatctct aggactcaac aaataatctc cattactttt tactacttct cctgtattta
    66241 tatctatcaa ctcatacttt tgtctacatg gctgtgcaca cctacctcta tttccagacc
    66301 tattgcctat catactactc ataaggcatt gacccgagta acatacacat aaagctccat
    66361 gtacaaaaac ttctatatct acagttgtat tttcacaaat ccattttatc tcatcaacat
    66421 ttaattctct tgctaaaaca actctatcaa accctacact ctgaaggtat tttacatctt
    66481 ctaaggaatg tgctaccatt tgagtgcttg catgtagttc aaagtcaggt aatagttttt
    66541 ttataagtct tgccattcct atatcctgta ctattaaagc atcaatatct atatcgtata
    66601 aaaattttac atactctaca aaatcttcta tttcatactg ttttatcaat gtatttacag
    66661 taacaaagac ttgcactccc cttatatgag agtatttaac agcctcaatc agctcatttc
    66721 tatcaaagtt atttgcagat gctctagcac taaagtcttt accacctaaa tacacagcat
    66781 ttgctccgtt ttgaactgct gcttttaatg aatcaaaaga gcctactggt gcaagtaatt
    66841 ctattttttt catatttttc tcctctattt atacttgtta ggttaatata attatatcta
    66901 taacttaaat atttgtatta aatttatata tttcttacta tattttaaat tcttcaatca
    66961 atttatatat tagttattta tatttggttc atttgagtta acatatacat tatttactat
    67021 atttagtata tttctattaa cttatatatt gattactgta ttttaaattc ttcaattaat
    67081 ttatatactg cttactaaat ttaaaacatt ttaattaatt tgtacactg
//
LOCUS       DAELJO010000004        60098 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELJO010000004 DAELJO010000000
VERSION     DAELJO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.004"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(83..1684)
                     /locus_tag="KPB88_000197"
     CDS             complement(83..1684)
                     /locus_tag="KPB88_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0052511.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIEMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1795..2472)
                     /locus_tag="KPB88_000198"
     CDS             complement(1795..2472)
                     /locus_tag="KPB88_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052512.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2485..3180)
                     /locus_tag="KPB88_000199"
     CDS             complement(2485..3180)
                     /locus_tag="KPB88_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0052513.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3232..4431)
                     /locus_tag="KPB88_000200"
     CDS             complement(3232..4431)
                     /locus_tag="KPB88_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0052514.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4692..6737)
                     /locus_tag="KPB88_000201"
     CDS             complement(4692..6737)
                     /locus_tag="KPB88_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0052515.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNDTLVNENFDSIILDFKQSNFFEESICILASIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7148..7957)
                     /locus_tag="KPB88_000202"
     CDS             complement(7148..7957)
                     /locus_tag="KPB88_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0052516.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8041..8574)
                     /locus_tag="KPB88_000203"
     CDS             complement(8041..8574)
                     /locus_tag="KPB88_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0052517.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8724..10073)
                     /locus_tag="KPB88_000204"
     CDS             complement(8724..10073)
                     /locus_tag="KPB88_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0052518.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKYIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10085..11467)
                     /locus_tag="KPB88_000205"
     CDS             complement(10085..11467)
                     /locus_tag="KPB88_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0052519.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     TYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11711..12925
                     /locus_tag="KPB88_000206"
     CDS             11711..12925
                     /locus_tag="KPB88_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0052520.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDSKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13102..15015
                     /locus_tag="KPB88_000207"
     CDS             13102..15015
                     /locus_tag="KPB88_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0052521.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYKNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYGKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15286..16260
                     /locus_tag="KPB88_000208"
     CDS             15286..16260
                     /locus_tag="KPB88_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0052522.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16281..16556
                     /locus_tag="KPB88_000209"
     CDS             16281..16556
                     /locus_tag="KPB88_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0052523.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16569..17732
                     /locus_tag="KPB88_000210"
     CDS             16569..17732
                     /locus_tag="KPB88_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0052524.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18069..20348
                     /locus_tag="KPB88_000211"
     CDS             18069..20348
                     /locus_tag="KPB88_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0052525.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKITHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20443..21783)
                     /locus_tag="KPB88_000212"
     CDS             complement(20443..21783)
                     /locus_tag="KPB88_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0052526.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21795..22577)
                     /locus_tag="KPB88_000213"
     CDS             complement(21795..22577)
                     /locus_tag="KPB88_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052527.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22592..23614)
                     /locus_tag="KPB88_000214"
     CDS             complement(22592..23614)
                     /locus_tag="KPB88_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0052528.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23620..24633)
                     /locus_tag="KPB88_000215"
     CDS             complement(23620..24633)
                     /locus_tag="KPB88_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0052529.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24761..25699)
                     /locus_tag="KPB88_000216"
     CDS             complement(24761..25699)
                     /locus_tag="KPB88_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0052530.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            25969..26433
                     /locus_tag="KPB88_000217"
     CDS             25969..26433
                     /locus_tag="KPB88_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052531.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26545..26862)
                     /locus_tag="KPB88_000218"
     CDS             complement(26545..26862)
                     /locus_tag="KPB88_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0052532.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKAREYIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26875..27606)
                     /gene="chbG"
                     /locus_tag="KPB88_000219"
     CDS             complement(26875..27606)
                     /gene="chbG"
                     /locus_tag="KPB88_000219"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG0052533.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27632..28942)
                     /locus_tag="KPB88_000220"
     CDS             complement(27632..28942)
                     /locus_tag="KPB88_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0052534.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWVVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29019..30332)
                     /locus_tag="KPB88_000221"
     CDS             complement(29019..30332)
                     /locus_tag="KPB88_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0052535.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEVYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEVGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30354..30665)
                     /locus_tag="KPB88_000222"
     CDS             complement(30354..30665)
                     /locus_tag="KPB88_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052536.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30865..31569)
                     /locus_tag="KPB88_000223"
     CDS             complement(30865..31569)
                     /locus_tag="KPB88_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0052537.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31817..33475)
                     /gene="treC"
                     /locus_tag="KPB88_000224"
     CDS             complement(31817..33475)
                     /gene="treC"
                     /locus_tag="KPB88_000224"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0052538.1"
                     /translation="MLDFKKSVVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKKAKKRNMGIMLDIVFNHT
                     STEHEWFKKALKGDKYYKDFYIFKNPKESKSAPTNWESKFGGNAWEFVEKFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQGDGRH
                     FYTDGRKVHQYLKEMCEESGVLENDMLTVGEMSSTTIENCIKYSNPNEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKLLNEWQIGMEKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYVYQGEEIGMTNAYFTNIKQYVDVESLNYFDILKS
                     QGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKPWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIELRKKYDVVAYGDFKPLLEENDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVILDNEELEKYKCIISNYGDRKVTSTITLRPYECMALFKNKK"
     gene            complement(33538..34971)
                     /gene="treB"
                     /locus_tag="KPB88_000225"
     CDS             complement(33538..34971)
                     /gene="treB"
                     /locus_tag="KPB88_000225"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBG0052539.1"
                     /translation="MGKNKILNSKESISQIIEYVGGKENIQSATHCVTRLRLILKDTN
                     KANINKLEGIDIVKGCFNAGGQFQIVIGVGVDKVYKDLIEQTGAEEVSKEEIKQQVNE
                     KSNKLQKFIRVLADIFIPILPAIVASGLLMGVNNMIANPGIFYEQSVIEVNTGIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILTKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVYLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            35219..36958
                     /locus_tag="KPB88_000226"
     CDS             35219..36958
                     /locus_tag="KPB88_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052540.1"
                     /translation="MNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKQNMDVLRII
                     PTVNNIPVDYEFEADYFSILIKFNNGSISICFDGDSKILFYGKGKNVGLKLDTLPVYN
                     FEYNYLMGKKDSEYCVINSYKNLTKFLVFSVDGKVNLSQNIFIDDCASTNKANNTSII
                     DVNNNTDNEFTCIIEDIPTHMKVPDYKEYNFETSLNNTKKLFNEYYLAQPKVDEKYKN
                     SLLRASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVDDKLAFDQ
                     MAVLFDYQDEYGQIPGSLSDSTIRWNFTKPPIQGLFFNKMMKKINFTKETLEEIYNYI
                     EKQVLFYIKFKDSNMDGIFEYHHGNDSGQDNSTIFKGVMVVDTPDLTAFIINAMDMLA
                     KVAEKLNKYSEKEYWANKADELTKLFLSYFIVDDLPVARETLTGKILPNNALLPLISL
                     VLGNRLPKYTRDKMIDLIISERYLTKWGIATEAIDSDEYQDDAYWRGPIWAPTTLLFV
                     EALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTFIYLAAK
                     VYEYNTQRINNIK"
     gene            complement(36974..37705)
                     /locus_tag="KPB88_000227"
     CDS             complement(36974..37705)
                     /locus_tag="KPB88_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBG0052541.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYKLIEEYSCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYRANTQSEFLLGGTESFKESAIRNNKEYKTKVV
                     HFEEIIIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMENELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFQQSQRINNIK"
     gene            complement(38147..39865)
                     /locus_tag="KPB88_000228"
     CDS             complement(38147..39865)
                     /locus_tag="KPB88_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0052542.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(39870..40859)
                     /locus_tag="KPB88_000229"
     CDS             complement(39870..40859)
                     /locus_tag="KPB88_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0052543.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(40955..42004)
                     /locus_tag="KPB88_000230"
     CDS             complement(40955..42004)
                     /locus_tag="KPB88_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0052544.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(42282..42950)
                     /locus_tag="KPB88_000231"
     CDS             complement(42282..42950)
                     /locus_tag="KPB88_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG0052545.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(42956..44329)
                     /locus_tag="KPB88_000232"
     CDS             complement(42956..44329)
                     /locus_tag="KPB88_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG0052546.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(44326..46104)
                     /locus_tag="KPB88_000233"
     CDS             complement(44326..46104)
                     /locus_tag="KPB88_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG0052547.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(46221..46544)
                     /locus_tag="KPB88_000234"
     CDS             complement(46221..46544)
                     /locus_tag="KPB88_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG0052548.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(46531..47508)
                     /locus_tag="KPB88_000235"
     CDS             complement(46531..47508)
                     /locus_tag="KPB88_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG0052549.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(47523..48086)
                     /locus_tag="KPB88_000236"
     CDS             complement(47523..48086)
                     /locus_tag="KPB88_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG0052550.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(48138..48629)
                     /locus_tag="KPB88_000237"
     CDS             complement(48138..48629)
                     /locus_tag="KPB88_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG0052551.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(48632..50557)
                     /locus_tag="KPB88_000238"
     CDS             complement(48632..50557)
                     /locus_tag="KPB88_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG0052552.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(50559..50870)
                     /locus_tag="KPB88_000239"
     CDS             complement(50559..50870)
                     /locus_tag="KPB88_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052553.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(51500..51775)
                     /locus_tag="KPB88_000240"
     CDS             complement(51500..51775)
                     /locus_tag="KPB88_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG0052554.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(51890..53299)
                     /locus_tag="KPB88_000241"
     CDS             complement(51890..53299)
                     /locus_tag="KPB88_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG0052555.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKVSGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(53670..54332)
                     /locus_tag="KPB88_000242"
     CDS             complement(53670..54332)
                     /locus_tag="KPB88_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG0052556.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(54586..56805)
                     /locus_tag="KPB88_000243"
     CDS             complement(54586..56805)
                     /locus_tag="KPB88_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0052557.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(57081..59735)
                     /gene="adhE"
                     /locus_tag="KPB88_000244"
                     /gene_synonym="adhC"
     CDS             complement(57081..59735)
                     /gene="adhE"
                     /locus_tag="KPB88_000244"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0052558.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALERYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        19891 a        10462 c         7215 g        22530 t
ORIGIN      
        1 tttttattta aataaatctc aaacttcgtc tttacttact tagatattta tttactttct
       61 aaatcctatt atttttaaaa cattatcttt ttaatacttc accacattta ttctcagtaa
      121 tctctccatt ttccaacgca atttttccat ttacaattac atattcaaca ccattggcta
      181 gtaattgtgg ctttgaaaaa tcaatatctt ctgacctaga ataactatag ttatccattt
      241 caataactgc tatatcagct atcaaccctt ctttaagaat acctctattt tttattttaa
      301 attgcctagc tggtaatgaa gtaactcttc ttacaatctc ttccaatcta acaccagact
      361 ttctgtacat attgaagaaa attggaaaag ctcctcttct ttgtagttca aaccatgaca
      421 tatccccacc cacactaaac aaacaatctg aaccaaccat aacaaatgga ttatcaatta
      481 ttttatcatc atgtagttta tctgggaaat cagctctata cattcctcct aaacaaaaga
      541 taagttcttc gtctccatct aaaagtaaat cgagtaataa cttgtcttca tctattcctt
      601 tttgtgtagc tatttcacgt attgatttct tttccatatt aatatcttta gtgtttagaa
      661 gtattatact ccccctatct ccaagtataa atgcttcttt taatataagt gttcttccct
      721 cttcatttct aagagcatcc attaccccat ttatacctaa ttcaaataga ctagatgata
      781 ttgccataat aaattgaatt actctttttt tcttcataca atgcccagaa cttcttggta
      841 ctgtgtcaac cattatttca acaccattat acctagcctt taatatctca tcaaatgctt
      901 ctatttcaac tggacttaag tgagatactt gaaccctagc accacttttt tgcccaacct
      961 ctatggcttc ttttactgca ttatatcttc ctatgtaatc tctaatatga gatgtatata
     1021 taccatcatg ctctttaatc tgccttgcaa tatcaacaag ttcatcaata tcagcatact
     1081 tacttggtat atatgccaat ccagtagata ttccaaaagc accacttttc attccatcat
     1141 ttattatatc tgtaatttca tttttttcct tttctgtagg aggtctatcc ttagaacctc
     1201 ccataacact ccatcttata gtcccatgcc ccaacaagaa tcccatattg acagaaatac
     1261 cacttttaga tataaggtca caatattctg taaaattatt ccaacattta tttttatcta
     1321 taagatactt tttttcttct tcaagtaata gaccattttt atacatatat tcatatacat
     1381 tttcagaaga atatggtgtt atagaatgtc cacaattacc atttatagtt gttgtaactc
     1441 cttgttttag aaacttttca aatttaccat ctagtattgc tactatttct tcatgaacat
     1501 gagggtcaat aaatccaggt gttataactt ttccacaaca atcaatctct ttatttgtat
     1561 tgctatttat cttagaatct atttttgcta ttaagttgtc tttaattgct atatctccat
     1621 aaaaaccagg attacctgta ccacctatta taaatccatt ttttaagatt aaatcatata
     1681 tcaaaatatt ccctccttaa aaaccaattt ttaaataatc ttaatgtaat ttttatattt
     1741 gaaaatcaaa ttattaaatt tcatatttga aaattcacta ttatttcatt gtaattagtc
     1801 actaagctta tctttataca tagctcttgc aattaatata gtaccaaata cacatataag
     1861 aactactaga gactgtggta ttataccttg ctttgataat ataatagtca atacagatat
     1921 tacaattgct actagaccta attttttatc ttgtaaaaat acttgtccaa aaactgaccc
     1981 aaatagagct ggtaaaatta agtttaattt ttctactact tcagctggaa ttttagatag
     2041 tacgcttcct cctaaaatta cccctatagt tagtatactt atattcacta aaatagatac
     2101 agctattcct attgtagata ttatcgagcc ttcttctgta cctgattcaa cttctgctgc
     2161 cttttgagct gctattgaac atggtagcct catattagat atattaccag aaagaaaact
     2221 catataagtt cctggtatac ctaagattgg tgaataagaa actggttcta ttatgtaatt
     2281 tggagcactc atactgacaa ttgccattgt acttgctaat aatgctgata ttggaggcat
     2341 aataccaaat ccaaagctca aaattattcc aggaactaat gctgctataa tacccaaaga
     2401 tagtgttagt cttccatacc ttatcataaa aggtaaatat tcttcattgt atatagattt
     2461 tactttactc ataaaactcc ccccttatat tactgaagct attatcattc cgctaaacat
     2521 tgatattgta agcccccact ctcttaacca ttttatattt ttcttctttt caagcataca
     2581 caatattacc attatagcca ttccacttat acatgcaatt gttccattat caaaacgcaa
     2641 caccctatca gcatttagat atgcaaatga acccaacatt gctcctagag atattattgg
     2701 tataaatgat ttcttgccac tagatataac attggttatc ttatctagtt tatgtccaaa
     2761 tagtaatgtg aatattatcc atcctaaaga acctaatatc atagtccaaa ctgcatttgt
     2821 aaatgcaagt tttgtcattt caggagaacc taaagttata cctaatgcat ctgttccaaa
     2881 tccggctgac attgattcaa aaattacaga acctataaat gataatctca tccatgctat
     2941 aggcccaccc atagttacta taagcgatac cattccagca aatatagtca ttgaaggccc
     3001 aatagctgaa atagcactac tcttaacagc tgactttatt tgtttatctg ttattcccat
     3061 tgttttacca actttgcaag attttatagc aaatataagg gattgtacca ctactattga
     3121 tactgctact gtactcgcta tccacaaaag tggatgatta gctaatttta aataatccat
     3181 ttataaaacc tccctataaa tcctcaaatt ttattaatat ttctaatata tctatttcca
     3241 aacctgtttt agtactctaa gtatatttcc acctagtacc ttctctattt cctcatcact
     3301 atatccatgc tttactaaat atctgacaat gttttttgaa gtttctgttg ggttctcaag
     3361 accttttaca tattctactt tgggatgttc ttttgtacct gttatttttt ttatatcaaa
     3421 tttagcagct agtactgagt gtaattttac atggtcgcca tatactgtat ctggtccaaa
     3481 agctacatgg tctattccta ctaaattttt aatatactca aaatgttcca taaatgactc
     3541 tattgaatgt tctttattct tctctgttat tgtagtatga ggtgctgctt ctacacctat
     3601 aacccctcct tttttagcac atgctattaa tacttcatct ggagttagtc tctttgtgtt
     3661 ccataaactt cttgccccaa catggcttaa tattattggg tccttgctat attctattac
     3721 atcaagtgta gtttgaactc caacgtgaga gcaatctatt gccattccaa ttttattcat
     3781 tctttcaaca gcttttttcc cgaaatttgt aagacctcca tctttctctt cttttagtcc
     3841 tgaacctaat gcattggatt cgctgtatgt tatacccatt aaccttatac caaatccata
     3901 cagtatatct attctatcaa gttcattttc tattggtgct gcaccttcta atgttggtat
     3961 caatgctact ttaccttcac ttttagctct gtatatatct tctacttttt cacactttat
     4021 gacaaaatct tgatgagcta aatcacataa tctcatgcct aaatctatta atatatcatc
     4081 ccatttccac cctgaatttg atgttatcgt acatacacca tccattaaat tatcaaatat
     4141 agcatcataa tatcctcttg ataatgcttc ataagcacat ctttgtttac cttccctatt
     4201 atattcaaat atatctcttt ctaggtgttc tggaaataat actggatgtt catgtaatga
     4261 tataaatatt ttttcatcca ctatactttt aaccttagct tcttgaatat catctaattc
     4321 aataaggtat tctttaactc ttttatctcc tttacacatc tcaatagatg aaatatcttt
     4381 tcctttttct aaataactat aggcttcata tcctttgtaa gctgacttca taacaacacc
     4441 ctccaagttc tacaattttt tatatttttc ctatgtagca attgatgtgc caaaatataa
     4501 attttaattg tataataaat cttgcaaaaa ataaaaacag tagtaaggtt gatatttact
     4561 actgttttat taaaaataat cttcttagat tatttattca atatttttga attatcttaa
     4621 tcttaaagat aattttattt atttctatcc attataggga tttttttagg gtgttttgcc
     4681 tttttagtcc cctatatcac tcccttaatt tttacatatg atataaagtt ctttccttca
     4741 tcagttataa aactaccttg tttttttact ccagataaaa gtaaaccatg gctctttaac
     4801 ttatctattc tttttctaat ttggtctaca cttaaaacta tatctttttc ttttaaaatc
     4861 tcttgcagtt tatttcttcc taataatata tttttattat tccaagtttc tatactggcc
     4921 aatatacata tactttcctc aaagaaatta gattgtttga aatctaagat aattgaatcg
     4981 aaattttcat taactaaagt atcgtttttt tcaaatctaa attgttctgg caaatgctcg
     5041 taatctactc tatcttcctc aactatagta tcaatataat aaattaaatt tttaagctct
     5101 cttacatttc ctggccattc atacaatgtt aaagcttgca ttgattcctc tgtaaaacat
     5161 ttgttgctat ttatttcatc taaaaaatac ttacttataa gaggtatatc ttccttacgt
     5221 tctctaagtc taggtatctc tatatgtaat acattaatcc tataatacaa atcttctcta
     5281 aaagaacctt ctttaatttt tttctttaaa tctttatttg ttgcagctat aattctaaca
     5341 tctattggta taattttact tccgcccatt ctcataactt ctttttcctg taatactctt
     5401 agcaatctct gctgtacatc taaagatata tcacctatct catctaaaaa aattgttcct
     5461 gtatgtgctc tttcaaatat acccattttc ccacctttta ttgctcctgt aaaacttcct
     5521 tcatcataac caaaaagttc actttcaata agtgtatctg acaaagaaga tagattaact
     5581 gcaacaaatg gcttgttttt tcttagagat tcattatgta ttgcctgggc aaatatttct
     5641 tttcctgttc cattttctcc aagtatcaac acagaaaagt ctgtagttgc tatttttctt
     5701 gcaatattaa ttttttcttt tattatttta ctctcaccta caacactctc aaatgtatac
     5761 tttgatacaa aaccttttga ctgaagttta ttttgaatct tgtcctcaag cacttgaatt
     5821 gcacttatat ttttaatgct tataatactc tttattcgtt cattattttc ataaacattt
     5881 actttattaa ttatcagttt tttgttattt aaatttacta cttcatcatt aacttcatct
     5941 tcttgaaaaa atattttttt gacaacctta tcactaaata agtccataaa gttattagat
     6001 ataatttcat tttggtctat tccaactaga ttagaaaata ctttgttgca atatataaat
     6061 tttcctaatt tatcaacaga agcaatacct tcatctataa tctccaaaaa actcttcatg
     6121 gttttattca tagttgtata atacctatat ccacttactg tatcttcatt gtatttttcc
     6181 ttaataatat gtaatttatc tatagatata tttagttttg taaagatttc tataactgta
     6241 tttatatcaa ttaccttatc acctatgtca attatttgct ttatgttttg tggtatgctg
     6301 tttctgcttc ccgtaataat tccaatctca catttagact tatcacaacc tggataatat
     6361 ggtattagat taatatgaat taatcctagt tttctaatta tcttggcagt ttcatctgct
     6421 gactctttat aagcatctat aaccataaca tcagaatcat tttctatact tattatctga
     6481 tttatatttt caatatttat agtacgatgt actattacaa taggtatatt tttatcaata
     6541 ttattatgta tatattcctt tatatcattg tctgaacata ctacaagctc atagcttgat
     6601 agttcaatat ttatcctatt tacatatacc aagttatcta tattacaaaa ttctccaaat
     6661 atattttcca attgttttga tatatctata tttattttgt catcagttga tacaaataaa
     6721 atattatgtt ttttcatagt cccacctcct gtttaacgag tatagaaagt aataatttgg
     6781 tctattgtag cactttttaa aaaagtttta aatcttaaaa atcaaatttt gtaccattta
     6841 ctattatttt ccataataaa ataatgtaag ctatcttttt tcacatataa tttaatttct
     6901 aaaacggtta gtttgacatt attggaatat aaataaaaat ttaatataaa ttcgttaata
     6961 tattaataca tttttaagaa tgacaaggaa taaattataa caaattttag gaatatataa
     7021 taatagggtc tcagaactta gttacaagtt ctaaaaccct atttgtttag tgtaaaaaac
     7081 atcataaaat ttagcttaaa tcttttcaca taatattact ctttttctaa gtgataataa
     7141 aatatatcta ttcctctaaa aactgtaaaa attcctgatt aaatagttta agctcatcat
     7201 agaatatagc atgaccacta tactcaaaag tatataaaat tgaatcttct attttttcat
     7261 tcataaaaac tgcaaattca tacggacata tttcatctaa tttcccatga aatataccag
     7321 taggcactct tacacatttt aaatcattaa ataattcttc atctcttaaa gatacagcag
     7381 ttccaatagt gccaatacct gatgcactaa aaccaatatc attaaaccat cttctaaagc
     7441 tttctggtgg atttgatgca aatacttttt cgccaaaatc ggttaccatt tctggtctat
     7501 cagtacatgc ttgtgctatc aatttattta catcttcacg tgtcatacca tatggaaatt
     7561 ctggaggtct ttgtacaaat gatggtgcag cagctgcagc taataccaat tttgacactc
     7621 catatccatt aaataaactc atatacctaa gtacgattgc tcctcccatt gaaaaaccaa
     7681 caagggtaaa gtcttttaat ccaatagcat gtaccacctt ataaatatcg tctgctaatt
     7741 ggctataagt atatcctcct gatgttgcat cagatttacc aaaacctctt aaatcaattg
     7801 aaactgttct atatccgagt tttggcaata tatttgtttg atattcaaat atcttatgtc
     7861 ctaagggcca accatgtata aaaagtacag tttttttagc actcggattc aaatcatata
     7921 ctgctatgtt tatatcattc acatatacat aatacatatc aacagcaact cccttcaaga
     7981 aaatgtatga tggttatttt atatatatga ataaaatgat gaataactaa attttcaaat
     8041 ttatttacaa tacttctcta ttgccttttc aaataaactt acatgaagtt tttcatctag
     8101 gattattcta tttaatatag cttttatata agggtcttca ataagactta tatctttata
     8161 ataattatta atagccacat gctccaaatg caaatctagt tttaatctgt tacaaataga
     8221 atttccataa catacaaaac caccattcca atatgcacca caagaacttg tagaacctct
     8281 atatacagga tttccaccca ataatcttat agtctttgct aatatctcca tatgtaacat
     8341 ttctgttata gatatattta tccacatttc acttaactct ctatgcgttt catctaaatc
     8401 aaaattatgg tatagatatt gatttacact cgtaaattca cttgatactc cggcatagtc
     8461 atccatcaat aattctgcat agtacttatt tggacctaaa acttttatct ctggatatgg
     8521 ctcatcactt gaatatccat ttcgcttgtg catgtcataa tgtgcatcac tcataatatc
     8581 tcctccttta aatttaatgt atgatattaa ttgtatgcat cacaactttt tcatataact
     8641 tagataataa aatctactta aataataaaa agccatatta attaaaatta acatgacctt
     8701 ttatccacaa aattactttt taattattca tcttttccag ctactgatag ctctttaata
     8761 acaactgaag gtgaacctat actactcatt ggaaattcta aatcttcccc tatagcttca
     8821 atatctttca acaggtcaaa atagtttcct gctactgtaa tttgctccac tggaaatact
     8881 ttttttcctt ctttgatata aaagcccttg gcagctaaag aaaagtctcc tgttactgaa
     8941 tttgcacctg aatgaagtcc tgcaaagcta gtaaccatca atccttcatc tatatacttt
     9001 ataatttcat ctaaagattt atctcctttc tctatataaa aattagttgg atatatgctt
     9061 ataggtgatg aatatgaaga ttttattcca tttcctgtag tctttacacc tgccttatta
     9121 gccgttttta agttatgaag taaagttata agctttccat ttaaaacaac ttctttttta
     9181 aatgtagcta ctccttcatc atcaaaagga gtagatgcca taccattttc taaaagtgga
     9241 tcatcaacta ttgtaactat aggagatgct atcatgtctc cttctctatt tttcaataaa
     9301 gataatcctt tttgagctga atctgcatta aatatcccag aaaatgtgct aagcaaagat
     9361 accatagctt cattaagcaa tatagtttta tatttacctg atggaatact cttaccacct
     9421 actttagata aagcattatc aacaccaagt tttgctatct cacaaggttt tatttcttca
     9481 atagaatttg ctatattata tcctattcca tcatattttt gtccattgtc ttctatgata
     9541 ggaactacaa accctataag catattagtt ttattgctta aattaagacc tttagtatta
     9601 gatataccat tacttgatac agaataagat atagtacatt tagataaatt taccacctta
     9661 tctgaataag ctttagtctc tctttccatt tctaaagcta aatcaatcaa cttatctgcc
     9721 tctaaatttt ctagctcttt agaataagtc tttacatcat tatagtgctt atctccttca
     9781 tatataaatt gtacatcttc attttcaata gtgtttactc catcttttac attttttatc
     9841 aacatatcaa cagctttatc atctaaaatt tctgtataag aataacctat ctttccattt
     9901 accttacctc taaaagataa accaaaagat ttatcaagat tgtatttctc aacttctcct
     9961 tcatatatat ttatacttaa attttctcct gtataataat atatttcaca ctcttcaaaa
    10021 ccttcactta gagccttttt aaataatata tctttaaaat ttttaagttc cacaattatc
    10081 cctcctatct tccacctaca gtaatttctt ttacccttat cataggttgg cctacattag
    10141 ttggtatact tcctgatgat gagccacaca tcccctgagc ttgttttacg ttattcccaa
    10201 ccatatctat attcataagt atatcgctac cttttccaat taaactagca cctctaactg
    10261 gctcttgtat ttctccattt tttattaagt aaccttctga aactgcaaaa ttaaactctc
    10321 ctgttacagg atttacagaa ccaccaccca tttttttagc ataaagtcca tctggtattg
    10381 actttataat atcttctggt ctatcttctc cagcagcaat ataagtgtta gtcatccttg
    10441 aagttggtgc aaatttgtag ttctgtctac gagaacttcc tgtaggattc attcccattc
    10501 gtctaccatt aagtttatct atcatataag acttcaaaat tccattttca atcagtatat
    10561 tcttttgagt tggatttcct tcatcatcta tattcataga accccagtag tttggtattg
    10621 taccatcatc tatagctgta atctttgtgg aagcaatctg ttgacctaat ttatctgcaa
    10681 atacagagtt cccctttgca actgatgtag cttccaaaga atgtccacaa gcttcatgaa
    10741 atattactcc accaaatcca ttatctattg ctaccatcat attacctgca ggacaatttt
    10801 tagcatgcaa catagtatgc gctactctag ctgattcttt tgcataatat tctgggtcta
    10861 tttcttcaaa tatttcaaac cctttacaac ccccaggacc ttcaaatcca gtttgatttt
    10921 cattgccctt agatgcaatt gaacttatcc ccagtctggt tcttattctt ctatcctcaa
    10981 catatgtacc ttcagtgttt gcaattaata ttttttggtc tttatccaaa tagctaacac
    11041 ttacttgaga tatatcatta ctaaattctt ttgcactctt atatgcaacc ttcataacac
    11101 ttactttatc tctatttcct attgaatttg gataaatttt tatattatgt atgttattta
    11161 ttttaatact gtcatttaat attacacttt ttccatcttc caattttcct aaagcaacag
    11221 cagctttgta tgctacatcc aacagactat ttaatgaatt gtcacttgta taagcatata
    11281 tacttttcaa tcctttaaat attcttattc ctatgccata atttcttcca cctattgcat
    11341 tttctacttt attatctatt aaactaatag aattatttat agtgtcttct tcaaaaatct
    11401 cagcaaaatc tcctccagta attagacact tttctaatac cttagaagct atattttttg
    11461 aaagcataaa tttacctcct actttaaatt tttaattaac ttttattcaa tctttttact
    11521 tgttaacaaa cattatatac tatattactt aaaataaggt aattatgaat atattataca
    11581 tatgttttat actaaaatgt tatcacattt tatctataca tacccatata actagataat
    11641 taaaatgtat aatattattt gctatagaaa ataatacact catacataat aaattttagg
    11701 agtgaagtat atgaatcaag aatatgtaaa aggaaatcct atttttacta atattaataa
    11761 agttccaaga caatttccat accttactga tgatattgat actgatgtta taattgttgg
    11821 tggtggcgta actggatgta tttgtgctta ctatctagct aaaaacaata taaagtctgt
    11881 cattcttgaa aaaggaagga ttgcacatgg aagcactagc gtgacaactt ctctattaca
    11941 gtatgaatta gatgacaatt tgatagattt aacagaggtt atgactctaa aagatgctct
    12001 aaaagcatat aacctttgta cttcagcact tgaagaatta gatacattta tagagctata
    12061 tggaaataag tgtgattatg ccaaaagaga tactctactt tatactgcca acaaacttga
    12121 agttaaggct atcaaagaag aatataactt aagaaaagaa aatggatttg atgttgaata
    12181 tattgatgaa tccacaaacc cttttagctt tgatttaaaa tcaggattga tagcaaagaa
    12241 tggaggacga gaattagacc catataaata tagtcaccat ttgattgatg taagtttaaa
    12301 aaatggtctt caagtatatg aaaatacaga agtaaaaaaa gtagatttat caaatgataa
    12361 ggttactgct gaagtatcgt atggatacaa agttcatggt aaaaaattaa ttgtagctac
    12421 agggtacaat acaagtttat ttacaaaaag aaattttgca actaagtcaa atacttttaa
    12481 tatagcaact aaaccactta aaaacatagc ttcttggaaa aacaacattt taattagaga
    12541 taactgtgac ccatataatt atctaagaac tactaaagac aatcgattaa ttataggtgg
    12601 tgaagatgta agttttgatg atatagaaaa tgaaacttta gccaatgaaa agtacgatat
    12661 tttagaacaa agattgaaaa gcatgtttaa ggatataaaa gacattgaag ttgaatataa
    12721 atattgtggt tgttttgctt caactttaga taatttaggt tttattggtc cagatagtaa
    12781 aaataacaat ctatggtatt gcttaggtta tggagcaaat ggtatactct ttgcaatatt
    12841 aggaggtata atgttaagtg aattatatct tggcaaacaa aataaaaata tgaaattatt
    12901 taaagtagat agatttgata aatagatatt atatattaaa agactgtaaa aaggtgaaac
    12961 tttataattc taaaagttaa ctttgataca gtcttttata atgttccttt taaaaatact
    13021 ttatttataa ttaaatataa agtaaaatgt taaaatataa ctgataaaat ttagtcataa
    13081 gataatattt aagtttattt gatgaaatat ggaggaatta ttatgaagga aatactcgtt
    13141 attgctccca ctaaaggaac ctatgaaaaa tcaattcaca tagttaaaaa aaacaaatat
    13201 aaaaatattg atgttgtatt tggcaatcta aaagaaggta tacctcttgc tgaaaaaagt
    13261 attaatcatg gtactagaat tatcataagc cgaggtggaa cttacaatat gttaaaagct
    13321 acttacaata tacctatagt tgaaattaag gtagatgctt atgatattat aaaaagctac
    13381 aaggaggtaa aaaattccaa cgaaccattt ggaataattg gatttaataa tgtcatctac
    13441 ggtttcgata ttatagaaga aatattgaat aagaaaataa ctatgattga aattgaaaaa
    13501 gaagaagaaa tttatgatgc tatagaaaag tacagaaaga aaggtataaa cacatatata
    13561 ggagatacca ctgttgcaca tattgttaag agacttaatt gtaagggaat attaatcgaa
    13621 tctagagaag aaaacatact tagagccatt caacaagcag aacaaatact cgaagcaacc
    13681 aaggatgaac aaaaaagaag attacagata gaagctatga cagattttgt acatgatgga
    13741 ataataacag ttgataaaaa ttttaaaata acattattta ataagagtgc tgaaaaaata
    13801 tttggtataa aaaaagaaaa tgcactttat cataatgttg tagatgttat cccaaacaca
    13861 gttcttccac aagtagttga gactatggaa cctcaaattg gtgaaataca aaatataggg
    13921 agtacaaaaa ttattacaaa tagagttcca ataattgttg atggtcagat tcaaggggct
    13981 gtttcaacat ttcaagatag caaggagata agttcttttg aacatacaat aagaaggagc
    14041 ttattaagca aaggtctatc tgctaaatac acttttgatg atataattca tgctagcgaa
    14101 aagacaaaag attgtataga acttgcaaaa aaatatgctt gttatgatac tcctatgatt
    14161 attactggag aatctggagt tggtaaagaa ctattttgtc aaagtataca taactatggt
    14221 aagcgtaaaa atgcaccttt tgttgctgta aattgtgctg ccatacctcc atctctaata
    14281 gagagtgagt tttttggtta tgaggaaggt tcatttacag gtgctagaaa aaaaggtaaa
    14341 gcaggtgtat tcgaactagc acatgaaggc acaatcttcc tagatgaaat aagtgaaatc
    14401 cctcttgaac ttcaaggtcg attattgagg gtattgcagg aaaaacaagt tatgagaatt
    14461 ggtggtgata aagttattcc tatagatgta aaaattatct gtgcttcaaa taaagactta
    14521 aaaaatatga tgcgcaatgg tttattcaga caagaccttt attttagaat aaatatacta
    14581 acattatatc ttccttcatt aaaagaaaga aaagaagata ttataaaatt atcagaattt
    14641 tttatacata aatattcaag caaatacaat aaaacgcctt taattatcac tccaaatatt
    14701 aaaaaggcac ttcttgaacg agaatatgaa ggtaatataa gggaattgga aggtatgatg
    14761 gaaagggctg ttattgtgga atcatttgac tcgattttat tagaaaagct tagcaatgaa
    14821 tatacaaata atatagttga taaagataat agtgattttg aatgcagtaa tttagattta
    14881 aaaaccttag aaaaaaaata tatctataaa atttataacg aaaataatag aaatactaca
    14941 aaaacctgtg aaattctaaa gataactcgc tccactctat ggcgtaagtt aaaagaaata
    15001 gaagatgatg tttaaaaata atacatatct atatatatca aaacgtttta tgtttaattt
    15061 taatacataa gacgtttatt tgttttcaat aaaaaataca attatatgat aaaaagaatc
    15121 tttgtaagtg aaattctcgt caatcatact tttaattata agtaatctac agcatttcaa
    15181 tcgattatta aaaataatca taataactaa agatacaaac tctatatatt ggcatagtaa
    15241 ttgcttttat aatacctata taaaaatgaa agggagtgat actagatgca catcttaaaa
    15301 tcagtgaaaa agataccagg aggacttatg gtctgtccct tgttatttgg agcactattt
    15361 aatacagtat gtccacaagc tcttgaaatt ggaggattca caacatcctt atttaaaact
    15421 ggtgcaatga gtatacttgc tctattttgt ctatgcaatg gagctcaaat caatatcaga
    15481 caagctggta aaccattgac taaaggtata gtacttacag caacaaaatt tataattggt
    15541 gcagtattag gtattattgt tagtaaattt attggaccta aaggtataat tggtattaca
    15601 cctcttgcca tagttgctac catgactaat tcaaatggag gtctatttgc tgctcttgca
    15661 ggtgaatatg gagactccac agatgttgga gcaatatcta tcttatctct aaatgatggt
    15721 ccttttttta caatgcttgc ttttggagta actggacttg caaatgtacc tatagtagca
    15781 ctatttgcag cacttatacc aatactactt ggatttatac ttggtaacct tgatgaagat
    15841 atacgagaat ttcttgcacc tggtacaaca ttattaattc ctttcttcgc tttcccttta
    15901 ggagcagcat taaactttaa tcaaattata actgctggac tacctggagt tgtacttggt
    15961 ttaggagtta ctataataac aggtatgggt gggtactttg taatgaaact tatacgtgca
    16021 gaacatccag cagtaggtgc agctacaggt acaacagccg gaaatgcagc tggtactcct
    16081 gaagctttag cagctataga ccctactcta gctacaatag cagctgtttc aacagtacag
    16141 gttgctgctg ctattattgt tacagcaata tgttgtccaa tgttagtttc ctatctagat
    16201 aaaagagaaa aaaagaaaaa atctattttt aaaacaaata aaaatattga agtaaattaa
    16261 tacttaagga ggtaaaatta atgaatgcaa ttattcatgc aaatgaaaaa gataatctag
    16321 ttacatgtgt cagaccttta gttaaaggtg aaaaagtttc aattgatgga aagtcttaca
    16381 tagtaaatga taatatacct gtctttcata agatggctac agaagatgta aaaaaaggtg
    16441 atgttgtata caaatacggt gaaatcatag gaattgcaac agttgacata aaaactggtg
    16501 actatgttca tgtacataac attgaaagta cacgtggtcg tggagataaa aaataggagg
    16561 aagttaaaat gaaattaatg ggttatttaa gagaaaatgg tcagtttggt attcgtaatc
    16621 atgttttaat tattccaaca tctgtatgtt caagtgagac agctacaaga atagcatcac
    16681 ttgtgcctgg agcaatagca atccctcatc aacatggatg ttgtcaaatt ggttctgata
    16741 tagagcttac agcaaagaca ttaataggat ttggaaaaaa tcctaatgtt gccgcagttt
    16801 tagtagttgg tcttggatgt gatggtattc aagcaaaaga attagcatct gaaatagcta
    16861 caacaggaaa aaaggtagat tacgttgtta tacaagaatg tggtggaaca ttaaaaacag
    16921 tttctaaggg agctgaaata gtaagtaaaa tggctcgtga agtgtcaaaa gaagtaagag
    16981 ttgaatttgg tatgagtgaa attacattag cacttgaatg tggtggttct gacccaacaa
    17041 gtggaatagc ttcaaaccct tcaataggcg ttgcatcaaa ccttcttgta gatgaaggtg
    17101 gaagtagcat actaagtgaa actacagaag taattggtgc agaacatttg ttggctaccc
    17161 gttttgaaga tgaagaaatg aaagataagt ttcttaaatt tgtaagtgat gtagaaaaaa
    17221 gagcaattgc tatgggagaa gatttacgta gtggtcaacc tactccaggc aataaagctg
    17281 gtggactttc aacaattgaa gaaaaatcac ttgggtgtat gtataaggca ggcaataagc
    17341 cattcaaagg tgctcttgaa tatgctgata ttgtacctcc tgataaaaaa ggattatatt
    17401 ttatggatac tccaggtcaa gatatagatt ctatcactgg tatggtagca ggtggtgcac
    17461 aaattgtaat cttttcaact ggtagaggta ctccaacagg aagccctatt tcaccagtta
    17521 ttaaaatcac tgggaatagt gatacataca acaaaatgcc agacaatatc gatataaatg
    17581 caggtagaat tataacagat ggtgctaaaa tagcagatat aggtcaagaa atatttaatg
    17641 aaataataga agtctgtaat ggtaaacata ccaaagctga aagtcttggt catagagaat
    17701 ttggcattta cagaatttca tctacatttt agattgtaac aaaatatctt ttataaatat
    17761 aatacttatg tagatacata agctaaaagt tctttctaat actataaaaa ttttctatac
    17821 tgaaaaggaa ctgtatcaaa attattttga aaacagttcc ttttttaacc tcttttactc
    17881 aaaatattat aaaataagat ttaaacaata aacgtatctt gattaatctc taagaagaca
    17941 taaataaagc taaatgtaaa gtgggataaa taaaatttaa tatctttgtg tgaaaagata
    18001 tttggtataa tatgtctgag agaattctta tttgtaaaca aataaaggat ggaggatgaa
    18061 acaaaagaat gaactttgaa aacacaaaaa ataaaaaaat aatagctact ttactattga
    18121 cactcctttt aatcgtgtca ttaacatgtg tcacttttat tatgaatcta aataaaataa
    18181 cacataatga aacagaacga tatcttgtag aagtttcaca aagtatttcc actacaatta
    18241 atgtgaaatg taaaagtata tttaggacac tatattctac gggagatact tattttgaga
    18301 ttaatgatag tgaaaaagaa gtcaatagat acttatcaaa aaaattaaaa ttatttgaat
    18361 ttgattggtt aggtattata gattctaatg gaaattttgt aagtgctctt aaagatacag
    18421 gtaatgttaa aggacaagac ttttatagaa aagcccttca aggcaaagaa acaattactc
    18481 ctgttaaatc acaatctggt acatatggaa ttttatattc tgtaccacta tacaatggcg
    18541 ataaaattgt tggagctgtt gctgcttgga atactattga tactatgaga aatttattaa
    18601 gtgtagaaag ctttcatggt gaaggatttt cccaaattat cgaccaagat ggaaatttta
    18661 ttgtaagttc agcaaacaaa aatgctccaa aaaatgtggt aaattttttt gacatgatta
    18721 aacaccaagg aaaacaggtt gataaaaata aacttgaaga aatgaaaata aacttaaaaa
    18781 agaacaaaac tggttctatg tattatacat taaatgatgg tatttctaag gcaatgcatt
    18841 atcttccatt acaagaagaa aacttatacc tcctttcagt tgttccaaca aaaacagcaa
    18901 gtgcacagtt tgatttactt ctaaaaaaag caatcttcat caatatttta attgttatac
    18961 tatttttatt gcttattata ttgattatgt ttataaatca aaagaacagg gcacaactta
    19021 taaaaatcgc ctatgtagac tctgtaacta aaggttacag taaagcacgt tttgatattg
    19081 aagctaaaaa aattatacag tcatcttcat cagaaagcta ttcattggta tctataaata
    19141 ttcagaaatt caaacttatt aatgattctt ttggtagtga agcaggagat aaaacattaa
    19201 agtacataca tgatgtcatt agtaattatc tagaaccagg agaattgctt tgtcgagttt
    19261 ctaatgacca gtttaatgta cttattaaaa ctattcctca aaaagaaatt cttaagtaca
    19321 tagaaaatat caccaatgat attaatagtt tcaataaaaa tgaagaatgt aaatatttta
    19381 tagctctatc tataggtgta tataaaattg atgacccaaa tctaccttta gtttcaattc
    19441 aagaccgtgc aaatgttgca cgaaaaaata ataaaggaaa cattggaaat catccacttt
    19501 atacatgtgt tttttatagt gatttagagc gtttaaaaat gattaaagaa aaagaaatgg
    19561 aaaatagaat ggaggaagca ttaaataata acgaatttat agtatatctt cagcctaaaa
    19621 taagcctcaa agataatact attgtaggtg cagaagcatt agtaagatgg caagacccaa
    19681 ataaaggtct tataccacca gacgagttta ttcctttctt tgaaaaaaat ggttttatta
    19741 caaaactcga ccactatgtt tttgaaaaag tatgtagtat gataagaaaa tggatagata
    19801 atggagaaac tccaatacct gtttcagtta atttttcacg tgctaattta aataatatag
    19861 attttttaga aaaatacaga atgcttcgta ataaatacaa cattcctgct aagctcttag
    19921 aaattgaatt aactgaaaca cttgtatttg aaaatctcca aaaattaatt aatataatag
    19981 accaaatcca tgaagaaggt tttcaatgtt cactagatga ctttggaagt ggatactctt
    20041 cactcaatat gttaaaagaa ataaaagttg acacattaaa acttgaccgt gcttttttta
    20101 gctcacctaa tgcagataat attgctgaaa atcatgtaat agaatctata gttaatttgg
    20161 caaaaaaatt aaatatgaat agtatatcag aaggtgttga aacaatcttg caaatggatt
    20221 atcttaaaaa aattaattgt gatatgatac aaggatatgt tttttcaaaa cctctacctc
    20281 aagaaatatt tgagaaaatg acttttggaa aaaaactaga agaaaccagt atggatagtt
    20341 ccgtttaaat aaaataaaaa acttgtctct ataaaataat tttaatcatt ttataaagac
    20401 aagtcttttt attttccaat ttaattttat tttctaaatt tcttacactc ggcttgtttt
    20461 aaattccctt tttaagaata ataatacgat tatcatagca ataaaatctg caactggctg
    20521 tgccaaccaa acaccatcta aattaaattg aattggaagt acaatgatta atggtataaa
    20581 taaaataaat tgtcttaaaa gagttaaaaa cattgaatgt ttaactttac ttatacactg
    20641 aaaatacaca ggtcctacaa tagaaatccc cattataggc aatgtaatta gatttaggct
    20701 tatacctctt actgctatat ccatcaaatc tgaatcttta ttaaatatac ctacaaatac
    20761 ttctgggaaa acttgtacta ctaaaaatcc aaatgacaat atgacaactg acactatgat
    20821 agataatatt aaagttttct ttgcacgctc atattgtttt gcaccataat tgtatgatat
    20881 tatagtttgc ataccctgat tcaatccaaa cacaggcatc ataaacatca ataaaataga
    20941 tgtaagtgct gtcatagctc caatagctaa atctccacca taaaccttta gagaattatt
    21001 tgtaaacatg tgagttatac ttatagctac ttccatagca aaaggtgtca ttcctattgc
    21061 aaataccttc tttactatat ctttattcaa ttttaagtta cattttttta gttttaaatt
    21121 ggacttccct cttataaaat aatgtattac ccaaaagaat actactattt gacagagtac
    21181 tgttgctata gctgctcctt ttatacctaa attaaataca aatataaata tagggtccaa
    21241 tatcaggttt aatacacatc ctacaatcat tgtccttgct gctaattttg gattaccttc
    21301 tgccctaatt gcattattaa gagaaaatgc aactaaatta aatatacttc caaccaatat
    21361 gacactcata tagtcttttg cataaaatat tgaatcttta cttgcaccca aaataaaaag
    21421 tatatcttct aagaaaacta atcctaatat agttataatt aaagagataa ttatagataa
    21481 tgtcaaagca ttacctaata tcttctctgc ttcttctcta tttctttcac ctaactttat
    21541 agacaatctt gtgctagcac ctactgatac caacattcca aaagctatta ttagagtaaa
    21601 tactggcata gttacaccaa gacctgcaat agctatagag ccaatacctt ccatagaacc
    21661 aataaaagct ctatctacta cattataaag tgaagttacc atcattgcta ttattgcagg
    21721 tatcgaatac ctaaataata aaatccatat tttttcatgt cttaatgctt cttgattctc
    21781 catgttgtca ccatttacac catgtcataa gttatacaaa ttggtttatt tgttcttggg
    21841 tctgttgcta tttctgcatc aatattaaat aattctttta gattatcctt cgtcataaca
    21901 tcatatgcat caccttcaca aactatttta cctttcttaa tacctatcat gtggtcagca
    21961 aatcttgctg cattattaag ttcatgtatt accatgacaa tagttcttcc ttgtttttta
    22021 tttagttcat ctaataattt tagtatctcc aattgatgtg ccaaatctaa atatgttgta
    22081 ggctcatcca gtaataatat atcagtttct tgtgccaaag ccatagctat ccatgccctt
    22141 tgtctttgtc ctcctgataa atcttccata tttctatcct taaattctgc aattccagta
    22201 acttctaaac tccaatttac aatctctctg tcttctttac tagaattacc aaatccactt
    22261 ttatgaggaa atcttccata agaaattaat tctgaaacgg taagaccact aggagcttga
    22321 gggctttgtg gaagtacagc catatctttt gctatttctt tagacttctt tttgtagatg
    22381 tcctctccat ttatatatat tttcccacta ttagggctaa taattctacc tatagtttta
    22441 agtattgtcg attttccaca accattagcc cctatgatgg tagttatttt tccttttggt
    22501 atatttaaat ttaaattttt tataatatct gtgtttccat atgatacatt tagattctta
    22561 gtatttatac agttcataac aacacccttt cctaatcatt taccaacatt aaataaataa
    22621 agtacggcac tcctattata gaaactacta tacctactgg tatttctata ggtgctagaa
    22681 gatttcttga tatagtatct gctattaaaa gtaataatgt tcctatcaat gctgatactg
    22741 gtattaatct tttatgcttt ggtcctatta tttttctacc tatatgtgga gctattaaac
    22801 ctaaaaatga aatactacct gctactgctg tcgatacacc agataaaatt actgcatata
    22861 ttataagtct tcttctttgt ttttctacat ctacaccaag acttatagat acttcatcac
    22921 ccaaatttaa aacatctata tatttagatt tataaatagt tatacccata aaaatcacta
    22981 taaatgggag cacagctatt acatatttcc aactggttcc ccaaatgctt cctgatgtcc
    23041 atgccataac cctattaaat tcttgtgttg taaatttcaa ttggaatatc gtcaaaattg
    23101 aagtaaaagc tatgtttaca cctataccaa taagtagtaa tcttgacgaa tttataccac
    23161 ctttccatgc aagagaatat ataagaaatg ctccaaataa agctcctata agtgcaacaa
    23221 taggcattgt aaatacagtc aagttactca taccttcata tacattacca ttcataagat
    23281 atatataaac tactacaaac aatgcagcac ctgaatttat tcccaaaatt ccagaatcag
    23341 ctaaatcatt ctttgtaact ccttgaagta ttacacctgc tgttgataat gcagaaccaa
    23401 ccaaaagtgc tattactatt cttggtaatc ttaatttaaa tattgcaatt tcatgattac
    23461 gctgaccctg ccctaaaaaa gtctttataa catccattgg ttctatagaa aatgagccca
    23521 tatttaaact caataaaaaa gtgataaaaa taagcaccat taaaattgat ataacaagta
    23581 atgttttaga atttattttt ataatatttt tcatagtacc tatttctccc cctttctaac
    23641 aaggtatata aatattggca ctcctataag tgctgttata gaacctactg gtgtctcata
    23701 tggaggattt atcatcctac ttaatatatc tgtatataca agcaatacag ctcctaatac
    23761 taaagagctc ggtattatat acttataatc tgaacctact attcctttag ctatttgagg
    23821 aactataaga cctataaaaa taatattacc agacactgac acagatgctc cagtcattaa
    23881 tattacaagt actatacata taaatctaac aaagttagtt ttttgtccta atcctattgc
    23941 cacttcttct ccaagactta atatagttat tcttggagcc ataataatag ctaaaataaa
    24001 tccaatacca ccagctacaa atagcaattt tataccttcc cacttcactc ctacaagccc
    24061 tcctgatatc cagaaactta attgttgaga taggttaaaa tacatagata ttgctgatgc
    24121 caaagaaatt agcaatgtac caagagcgac tcctgcaaga gctaacttta cattattaac
    24181 tcttcctctt gatttagaac ttataaaata tataaataat ccacttatac ttgctccaat
    24241 aaatgcaaac atcattacac taaaacttcc atgtataagt tgaggaagtt ttttttgaag
    24301 taccagtgat atcgaaattg cgaatgtagc tccttgtgta attcctataa cagatggttc
    24361 agctatagga tttctcgtta ttccttgcat tattgcccca gaaactgcta aaaatcctcc
    24421 aacaagtgca gctgctattg cccttggaag tctaacatcc tttactattc tcatatttat
    24481 tccatcttcc atttttatca aactatctat aacagttgat agattcatat cttttgcccc
    24541 aatagttatg gataatacaa ttccaaatac aagtacaact atacttacta atatcaacaa
    24601 ataagttata ttttttttac tcagcttagt caattatcct acctcctttt cccttttaat
    24661 cgtcagtata atatcatcta ttaagtattt taattttaag atttattaaa aataggtgtg
    24721 aaaaatgaat atcattatct cacacctact aagttaagaa ttactttgtt aattcatttt
    24781 taactgattc taataataac tcttttccaa ttggattgta tgattgactg aaaaatggag
    24841 cagcatcaag tattgttaca ttaccatctt ttactgctct tatttgagcc cagatagcac
    24901 tattttctaa atctttttta tctgcttctg tagcaattac aactatatgg tctgcatcta
    24961 tatctgttaa accttccata cttaccattg gtagtgttat tccatcctgt tttggcatat
    25021 tcttaggtct agctaatttc atatcatcat tgattagagt acctattcca ccatcactaa
    25081 ataccataaa ttgacctgaa cttgccaaca ctggtaaata agttttttct ccattctttt
    25141 ctataacttc cttacctaac tttgtagctt tttcatcata cttctgtagc caacttttag
    25201 cttcttcttc tttgtcaaat aactttgaaa catcagttaa cttgcttctc cagtcatttg
    25261 cataatcttt catcataact acaggagcta tttcttttaa ttggtcatat attttttctt
    25321 gtctttgact cattattatt aaatcaggat ttacttctaa tatagcttcc atatccatag
    25381 tatccatcat tgagtgccca actatcttta cacctttaag ctcttctctt atgtaacttg
    25441 gtaatttatc agtttcatat gagtctacat ttgctgttgc aacaggctta taacctgcta
    25501 ataatagttc ttcactactt cctgatatat caactatttt tttaggattt gatggtatct
    25561 taacctctcc tttaacagat tgtacaactc ttgtctctga tttatcttta tcctctgtat
    25621 tcttatctga acatgctgta acaagtacta acgtagttat aatagatata aatattgcaa
    25681 gtgattttat ttttttcatg attacttctc ctacttgttt attatactta tatgatattg
    25741 attatcataa gcacatattt attatagcat ttttctattt ttagtcaata ttattttgta
    25801 aaaggataaa aattcctaat taatcttaat tatatatagc ttagataagg ttttcttata
    25861 ttaaacagta tttttttaaa gaattggaca caaatacaat gacttttcag ttaaacaatg
    25921 ataaaatgca atttatagat aatgaattta gtaaggagga ttcaaaacat ggtacattta
    25981 gtatattgtg ataatgcagg aaaaaaagga gaaaaggtct tagataaaat cttggctgga
    26041 acaaaaacaa tggttattag aggtgctgct ggtagaaaaa taccacatag tagagtattt
    26101 gaagatgagt tactttattt tatggaaaaa ggaactgcaa aaatatctgc aaaagccact
    26161 gttaaaaagg tagaaaacta tgttaaatta acagaagatg aaatttctaa aattttaaat
    26221 gaaaatcaac caaaattaaa tttatctgaa aagcaaaaag ttcgttggca taaaaaatgt
    26281 ttatgcttag ttgaatttga aaatgttgaa gaaataacac ctcttgattt tgaccatcaa
    26341 ggaaatatgg atgattggct tattgttgaa aaaattgaag atgtagttgt tggtactagt
    26401 ataccttaca attatgagaa gtctaaattt taaagtatca ataacataat actatattgc
    26461 taaactctac ttaaataaaa aaagagtgca tttaataatt ataaaaatta tttttataca
    26521 ctctttttta ataataaatt taaattactt actttctaat ctttcatata catctataaa
    26581 ttcttctgct aattgttgaa aattcatagc tgtcattaaa tggtcttgtg catgaataag
    26641 tataacactt atttcagttt tattaccatt agcttcattt tgtatcaatt ctgtttggaa
    26701 tttatgagct ttatgaattg attctttcga cttttcaata tattctctag ctttatctat
    26761 attcccgctc tttgcttctt ttattgcttc aaatgctagc cctttcgcat cacctgcaaa
    26821 tccaattatt ccaaaagata tctctatcat cttctcatcc atttattcga cctcctaaat
    26881 atctctatag cttgatatga ctaatccatt cttttctaaa aattctttga cttttttaga
    26941 tgtaagtatc ttgtgctctt tcgttctatc aatagcataa gatgtagaat ttaaaatata
    27001 atcatctaaa aaagctgggt gagacataat atccactaca tcatacttca ttatttcgtc
    27061 tatatttttt ataaaatatt cttcacttac attttcctta taaaaactat ctattaaagg
    27121 aactacttta gaatactcta aattatattc aaatccacct cttattggaa gattatactt
    27181 ggttacaatc ttctctatta ctggctttaa acttactaaa gtatggatat gatggtgact
    27241 atctaaatga gatatatcta ttccaagccc ttttacccta tctatttgag cacaaagttc
    27301 tctataaatc tcatcacaat ccattttttc aataatttcg tctgtaatcc tcctaataaa
    27361 aaaaccgtct ttatcaacaa tagtttttaa gttagataat aatggtctac cacaacttaa
    27421 agtcatatga actccacaat ttaaagtttt attttcttta agtaagccta ctccatgttc
    27481 taccccaggc atatttgcca tcatagaagt agacctaact ataccattat tatgagctga
    27541 aattattcca tagttcacag cttcacaata tccaaaatca tctgcattta ttataatttt
    27601 agtcatacca taacctactt tctatagaca actatctatt aaatctagga agataatctt
    27661 tatgagcttc caacatttcg tctaatatag tttttgctaa gtcatcagat ggaatcaatg
    27721 ggtttataca aagtgccaaa agagcagatt cataatcgcc atatacagct gctttagctg
    27781 ccaatacttc aaatgatttg atttgactta ctaatccatg cacagaatct ggtaaatatc
    27841 ctatacttat tggttttgga ccattttttg ttattacact acttacttct actgcttgtt
    27901 catctttaaa atctcttata gaacccttat ttaatgtatt aactacttgg atatcttttt
    27961 tgtcattgta tatagaactt attaaattac aagcagcatc actatagtaa gcccctcctc
    28021 ttttttcaag ttgaggtggt tttacatcta atttttcatc cttatataat tcaaataatt
    28081 gttcttccaa ttctttaact acttgacctc tagcttttcc ctctttaaac tctctcaact
    28141 catcctcaag catttcttta gttttatagt agtatctatg atatggacaa ggtattgcac
    28201 ccaaagattt tacaaattca gcattaaaat caattgcctt tatattttgc atacttatat
    28261 ctgctttgac aaatttatca attgcttctt ttgtaacatc ttctccatct aaagatacat
    28321 ttagaccata aaccatatga ttaagacctg caaaatccat acttattctg tcactttcta
    28381 cattaaatag tttggcaaca tcatttttta aatgaattgg cacgttgcat agacctatat
    28441 atcttctaaa attagtatat ttgaatacag cctctgttat tattcctgtt ggatttgtaa
    28501 aattcaccac ccatgcattt ggacataatt cttccacatc ttttattata tcaaatataa
    28561 ctggtattgt gcgaagggct ttaaataaac ctccagcacc attagtttcc tgccccataa
    28621 ctccatgaga taaaggaata gtttcatctt ttattctcgc atctaaaaga cctactctaa
    28681 gttgtgttgt cacaaagtct gcatctctta gagcttctct tctatctaaa gtaagattta
    28741 ttttcatatc tactccagct ttttttacca tcctcttagc tagatttcca actatctcta
    28801 atttttcttt tccagcttct atatctacaa gccataattc ctttacaggt aactcttcat
    28861 atctatttat aaaaccctct acaagctcag gagtataact tgaacctcct ccaattgtaa
    28921 ctatttttag tccctttttc atctcattat accatcctta cctataattt aatatactct
    28981 acaaaactct atacatttat ttaccgaaat aaaactacct agttgttaat tttcaattta
    29041 ttagcttcca ttttttcaga cgctatgaca aatggaatat atattaatat tgataatatt
    29101 aaattaaata gtgctaaagc tccgccagtc cagtcacctg tagccattgc tccacctaat
    29161 ataggaggtg ttacccaagg tattttaggt aaaattactg gtgctactaa tccaaaatct
    29221 attgctaagt atgatacagc actacatatt actggtgcaa gtacaaatgg aatcataaat
    29281 ataggattta aaactattgg aagtccaaat atcaatggtt ctcctatgtt aaatattgca
    29341 ggtggaccac ctaatactat catctcttta cgtcttcttc cagctattaa taaagctact
    29401 attaatccca ataccattcc agaaccacct aaatatacaa atgcatcaaa gaatgcacct
    29461 gcaagtacat ggtatccttc cgttgctcct gcttgagcca aggcagcatt ttctccacct
    29521 aatttcataa gtatagtttc tgtaaaagga agtgctatat ttgcaccatg aagtccaaca
    29581 gtccaaagta tatgaattat aaatgcaata agcattgtag aacctagact atcagctaca
    29641 tttgatagcg gtgctcctaa ataagtattt aaaatatctg ttaagaaact tccattagaa
    29701 agcattttta taaatattcc tattacagta aatatcatta tagttaacat tcctggtatt
    29761 aactttgcaa atgatcttga aactgctggt ggaactccat ctggcatttt tatagttaat
    29821 ttctttacct tagataatct tacaaatatc tcagttgata ataatcctat taatatagaa
    29881 gagaataaag ctgctgtacc taagtaggtt tgttgtatca ttccccatcc ttcaactact
    29941 gtagttccta cctcaggaac tattttacca atttgtggag ccattataaa aaagatagat
    30001 aaagctataa gcccagattg taacccatca ctctcataag attttgctag aaaataagca
    30061 acacctatta ctaaaaatac tgccattgta cctattgctc cccaccaaag gtcgcctcca
    30121 aatgtggtcc agttttctcc aaatatactt gccatcaaat ttttgtatac ttccaatggt
    30181 aagttattta tcaatgttcc caatgcacct accatagtta taggtatcat tactataaat
    30241 gcatctctta ctgctactaa gtgcctttga gctcctattt tagcagctac aggcactata
    30301 tatttatcca taaatgacat gaatttttcc attttacatc ctccagtaca actttatttt
    30361 gaactattta actctacagc tctatctaac acagcttttc cattacatct tccataatcc
    30421 atcatattta taacttctac tggaatatta tatggttttg ccatttcagt tgcttttcct
    30481 aatacatatc taacttgagg ccctaaaagt aagacatcac aattttctat ttcatttttt
    30541 aagttcactt ctgatatagc ccatattttt gcttctatac cgttttcctt tgctgcatcc
    30601 tccatttttg taactaataa actagttgac attcctgctg aacaagctaa cattattttt
    30661 atcatttttt cccctccaaa ttagattcat taaatatttt ttaagtcata aattttttaa
    30721 actatacatt taaaatatgc ttttctatta attttttctt aatgtatata tcttattttt
    30781 aaatgtatat acatttaatt cttgatatta ttatatacca aatgtatata catttcaaga
    30841 aaactttttc attttattta aattttattc gacaacagca ttcaaagtta atgtatatcc
    30901 atagtgatat gtgtgtgagt actcaaaaat ctcactattt tgtaacttac aaatttggct
    30961 tatatataaa agtggctctg gttcttctaa ttttagatta tccatacact cttcattcga
    31021 tattacagct tgtacttctc tctctgacat ggctatcttg tatccacaca cttcttcaac
    31081 aaaattataa agagaatttt cacaatgagt cttatttaaa tttggaaata acttaactgg
    31141 catataagta atcatatatg aaactcttgc atcattggct aatcttactc tttttatttc
    31201 ccaaacattt tcttctaaac ttatattcaa cttacttgat aaattttcat ctgcctgaac
    31261 tatttcaaga gataccaata tattagatac tttatagcct ttactttcca tctctttagt
    31321 aaatccactt acagacgata tattatcata tatattattt gcaataacta tactacctac
    31381 acctctttgt ctagttatat agccttcttt aactagatta ttaagtgcag accttacagt
    31441 cattctcgtt acattaaatt cttcactcag ctgtttttca gaagggatta aatccccttc
    31501 tttcaaactg tctgaattta ttaactctct aacatgattt tctataactt tataaattgg
    31561 ctctttcatt taattcactc cttattaaat cttttatata ataagataat tttatataca
    31621 aacaaagaaa atgtctatac atttaagata atttaaattt tcttaatata gattataatt
    31681 tacatatgtg gaaaaatatt aaaataaggc tatgattttt acaatcatag ccttattaat
    31741 aaatattaaa ttataacttc aaaaataaaa tttatttaaa gagtacacat aaatttttct
    31801 taattttaat tttaaactat tttttatttt taaataaagc catgcactca taaggtctta
    31861 aggtaattgt tgaagtaact tttctgtctc cataattaga tattatacac ttgtattttt
    31921 caagctcttc attatctaaa ataacatcaa tttctttatc ataaaagtta tttaacacta
    31981 ctaaagattc tttttcgtaa catctcttat atgcaaatat agaatcattc tcttctaata
    32041 atggcttaaa atctccatat gctactacat catacttttt tcttaattct attaacttct
    32101 tataataata aaaaatagaa tccttatctt ttaaactatt ttctacattt atatttctat
    32161 aattatttat aacttctatc catggttttg attcgctaaa acctgcattc ttactattat
    32221 tccattgcat aggtgttctt gagttatcac gagatctttc ctgtaatata ttgtatatct
    32281 cgccatcttt cattccttga gattttaata tatcaaaata atttaaactc tcaacatcta
    32341 catattgttt tatattagta aagtatgcat tagtcatgcc tatttcttca ccttgatata
    32401 catatggtgt tcctcttaat aaataaatca tagtagctaa cattttagcc gattctttat
    32461 aatacttttt atcatctcca aacctagata ttatacgtgg ttggtcatgg ttacaccaaa
    32521 ataaagcgtt ccatccatta cctttttcca ttcctatttg ccactcatta agtagttttt
    32581 ttaacttaat aaaatcacaa tcttgcaaca tccatttatt ttggttttta taatcaactt
    32641 ttaaatgatg aaaattaaaa cacatagaca attctttctc atttggattt gaatatttta
    32701 tacagttttc tatagtcgta gaagacattt ctcctacggt taacatatca ttttccaata
    32761 ctccactttc ttcacacatt tcttttaagt attgatgtac ttttctacca tctgtataaa
    32821 aatgtctacc atcaccttgt aaatcatctt catatttctc tggtttagat attaaattta
    32881 ctacatcaaa tctaaatcct tttatacctt tatctatcca aaactttagt atatttttta
    32941 attcttttct tacattagga ttttcccagt ttaagtctgc ttgagtttta tcaaataaat
    33001 gtaaataata ttgattaaat ttttctacaa actcccaagc attgcctcca aattttgatt
    33061 cccaatttgt tggggcactc ttactttctt taggattttt aaatatataa aaatctttat
    33121 aatacttatc tcctttaagt gcttttttga accactcatg ctctgtagac gtatgattaa
    33181 atacgatatc aagcatgata cccatatttc tttttttagc tttttttatt aaattttcca
    33241 aatcatccat agttccatat atagggtcta tattataata atcagctaca tcatatccat
    33301 tatcattttg tggtgataca aaaaatgggg taatccacac ataatcaaca cctaaaaatt
    33361 ctaagtaatc taatttttct gttattccat ttaaatctcc taaaccatta tttgttgtat
    33421 cattaaacga ctttaaatat atttgataca ctacactctt tttaaaatct aacatattgt
    33481 ctcctatcta tccaatacat attaataccc cctgatataa aatcaaggga attatattta
    33541 tatgttgtca tttgttttaa atttaccact tttataaaat aagtatgtta atccaattgt
    33601 tattacaaat gttattgcca ttcctgtaaa atatactccc caatattgag gcattactga
    33661 aaggaatgaa aatactcctc caactccaac agctgtagct ttaacattgt gatatgatat
    33721 aaatgctcct cctattgcac ttgctaccat aacggctata aatggatatc taaatcttaa
    33781 gtttataccg tatatagctg gttctgttat tcctaaccat gctgatatag tagatgtaat
    33841 agatacacct cttaagtttt tattattttt aagtatgaaa aacattgcta atgtagcagc
    33901 tccttgagct aaagtaacag tctctgatat tggccacaat gttacatatc ctaaagtccc
    33961 tgccatttgt aagtttactc ctaagaataa atgatgcatt cctgttatta ctaatggtgg
    34021 tgaaataaat gaatataatg ctcctgcaaa aactggtgct acttcaaata aatacactac
    34081 tccattagtt atcatatttg cacctgtcat agttatagga cctataacta caaaagttaa
    34141 catacctgta attaataaag ctattggtga aactaatact aattgaactg catctgggac
    34201 agtctttttt aagaataatt caagtttagt taaaatgtat gtagctatta aaactggtat
    34261 tacttgacct tgatatccaa cttgagcaac cttcatacca aatatatccc atgttggtgc
    34321 tgcggatgga tttgctgcaa aatcatatgc agacatcaaa gctggatgaa ctaatacaag
    34381 acctaaaact acacctaaca aaggatttcc tccaaacttc ttggcagctg accaacctat
    34441 taatgctggt aaaaatgtaa atgatgtgtt tgctattaaa tttataatac ctgaaattcc
    34501 agctatacca gtatttacct ctatgacaga ctgctcataa aaaattcctg gattagctat
    34561 catgttatta acacccataa gtaaccctga tgcaactatt gctggtaata taggaatgaa
    34621 tatatcagca agaactctta taaacttttg taatttattt gacttttcat tgacttgttg
    34681 tttaatttct tctttagata cttcttctgc tcctgtttgt tctattaaat ctttatatac
    34741 tttatctact ccgacaccta ttactatttg aaactgtcca cctgcattga aacatccttt
    34801 tactatatca atgccttcta gtttattaat gtttgccttg ttagtatctt ttaatattaa
    34861 acgtaatctt gttacacagt gtgttgcact ttgtatattt tcttttcccc caacatactc
    34921 aataatttga gaaatgcttt cttttgaatt taatatctta ttcttcccca tattcattta
    34981 tcctttcttt ttttcatcat taccaacaag ttgtttagac aagtacttgt ttgaacaact
    35041 ttattgtaat attttgcatt aatttagtca atagtttttt gaaaacagtt tcctatttat
    35101 ttttttgata aattgactct tgataattat tttattattt ggtaagttaa atatatactt
    35161 acttgtataa acaagttaga ttctatataa aataaattat gaaaggaata tactacaaat
    35221 gaatataaaa gaaattccat ttagttactt tgggtcttat atgtctattt cttattcaga
    35281 tgaacttgga tttacattaa aatcactaag gggaaaatca aaacaaaata tggatgtact
    35341 aaggattata cctactgtaa ataacatacc tgtagattat gaatttgagg ctgattattt
    35401 cagtatatta attaaattca acaatggtag catatctata tgttttgatg gagattctaa
    35461 aatcttattc tacggtaagg gaaaaaatgt ggggcttaaa ttagatacac ttcctgtata
    35521 caattttgaa tataactatc ttatggggaa aaaagattca gaatattgtg tgataaatag
    35581 ctacaaaaat ttaactaagt ttttagtatt ctctgttgat ggtaaggtta atttatcaca
    35641 aaatatattt atagatgact gtgcttctac caataaagct aacaacactt caataataga
    35701 tgttaacaat aatactgata atgaatttac atgtattata gaggatattc ccactcatat
    35761 gaaggttcca gattataagg aatataattt tgaaacttca ttaaataaca caaaaaaatt
    35821 atttaatgaa tattatctag ctcaacctaa ggtagatgaa aagtataaaa attcactttt
    35881 aagagctagt tatataaatt ggtcttcttt cgttaaacct caaggatacc taaagagata
    35941 tactatactt gcttcaaact caacattctt aggggcgtgg agttgggatc actgttttaa
    36001 cgcattggct ttagctggtg tagatgataa attagctttt gaccaaatgg cagtactttt
    36061 cgactaccaa gatgaatatg gtcaaattcc agggtcttta agtgattcta ctattagatg
    36121 gaattttaca aaaccaccta ttcagggatt attctttaat aaaatgatga aaaaaattaa
    36181 ctttactaaa gaaacattag aggaaattta taattacata gaaaagcaag tattgtttta
    36241 tataaagttt aaagattcaa acatggatgg tatttttgaa tatcatcatg gaaatgattc
    36301 tggtcaagat aatagtacaa tatttaaagg agtcatggtt gttgatacgc ctgacttaac
    36361 agcatttata ataaatgcta tggacatgtt agcaaaagta gctgaaaaac taaataaata
    36421 tagtgaaaaa gagtattggg ctaataaagc agatgagctt acaaaattat tcttaagtta
    36481 ttttatagtt gatgatttac ctgtagctcg tgagacttta actggtaaaa tacttcctaa
    36541 taatgcttta ctccctctta tttctttagt gctaggaaat agacttccta aatatactag
    36601 agacaaaatg atagatttaa ttattagtga aagatattta actaaatggg gaatcgcaac
    36661 ggaagctata gatagtgatg aatatcaaga tgatgcttat tggagaggtc ctatttgggc
    36721 tcctactacg ctattatttg ttgaggcttt agaagaatgc ggtgaaaatg aacttgcaat
    36781 agatattgcc aaaagatact gtgaactagt tgataaaaat ggatttgctg aaaatttcaa
    36841 tgctattaca ggagaaggtc ttagagataa atcttttacc tggacagcta gtacattcat
    36901 atacttagct gctaaagttt atgagtataa cacccaaaga attaataata taaaatagag
    36961 tacaaattgt actctatttt atattattaa ttctttggga ttgttggaaa aatatgaatc
    37021 catcaggtat atgtctagac tgcgtatatt caaacattat tccttgagca ttataagtat
    37081 tattgctaac aactgcaaca caattataat ctcctaattc caaatatttt ttatctagtt
    37141 tagttgcttt ttcaactgtc atggttcttt ttgttgttac tatagtttca ttcaattcat
    37201 tttccatata ttcatatata gaattagatg ctatttcttc attaatatct tttactatat
    37261 ccttcataaa ataattatga tctataatag atggtttatt ttctaatatt cttactcttt
    37321 gtatataata cacttctgtt ccaacttcaa agttagttat ttgacttttt ttatcatcta
    37381 taataatttc ctcaaaatga actactttcg ttttatattc tttattattt ctaatagcgg
    37441 attctttaaa tgattctgtt cctcctaata aaaattctga ttgtgtatta gccctataaa
    37501 ttataaatac tccttttcca tgcttacttt gaacatatcc ttggtttgct aattcctgta
    37561 tagcccttct aattgtattt ctggaacaac tatattcttc tattaatttg tattctgatg
    37621 gtaataaatc ttgatataaa tattccttat tttctattct attttttaaa tctatatata
    37681 tatcataata tttggctctt cccattttaa cctcccaatt ttatatataa atatttaatt
    37741 gtgtcttatt tctaaaaatc atgtattaat ataatatatt ataagttaag caaaaaccaa
    37801 aataaatata aatttaaaac tggaactaaa ctttttataa aaaacttcaa ctgtaaaaac
    37861 tattaaaaca ttatttttat gcgaaactta ataatttgtc aaacttagct tgataatatt
    37921 ctaaatccaa ccaatcttcc agtaacacca aaaactttag atttagagaa agtttccata
    37981 gacatctctt tatttgataa atataattat gcatattttc aaatgtataa taaaaaagga
    38041 aaattaacac tattaatatt aattaaaatt taaaaaagtc tattctttaa ataataaatc
    38101 acaggaatag acttttttaa atttgtgttt ttataagata aattatttaa ttaaagtttg
    38161 ttcttttttc taaacgattc tttataaatt catatattac tttcactaat atatttgtga
    38221 ctaatattaa cacagctagt gctgctgcct gtgcagtact ccccttatca tctagttgta
    38281 caattgatat tgcagctact tttgaacttg cagtatatat aaaaactagt gctgatattg
    38341 tcaccatgga gtttaggaaa aagtacataa agttctctaa aactgctggc aaacaaagtg
    38401 gtatagttac gttcttaaga attgatgcgc tgtttattcc cattgattta gctaccatat
    38461 ccaattcttt atctactttc ttaatagcag tagtagctgt tataaatgct actgaaaaga
    38521 aatgaactat attacatata accattataa gtaatgttcc atataatcca ttaaacaaat
    38581 ttttaatgta tattgtatcc ataaaattaa actctaattt attaaaaaat aatacataag
    38641 ctatacctaa aactaatcct ggtaatgcca taggcaatgt acttaaaaaa tacccaaatg
    38701 attttaaata tggagctttt tctgtttttt cattcaaata tgcaaataag aaaacaaagc
    38761 tcgtccctag aatagctgtc aatagtgaca tttttattga atttataaat ggtttaatcc
    38821 catcaatact gtttttcata gaaaaatgtt ccagtgtcag tcttaaatca tatggccaaa
    38881 ctgatattaa tgaagataag attacagtta acattacagc tattatacca aatgcaataa
    38941 ttcctacaac ccctcctaaa atactatctc ttaacttatt agttttaacc ttatatggaa
    39001 ttgactttga agttatatag gaattttgat ttttttcaat ttttttagtt actataaacg
    39061 atattattgc aggtattaaa agtaatattg atacagttgc tcccattggc atattctgtt
    39121 gccctataat ttgcttatat acatctattg ataatacatt aaactctcct cctatcacct
    39181 ttggagctcc aaaatctgta aaacataatg taaacgatac gaatgcagaa cttattaaag
    39241 agtattttat acttggtatt gttatgctta taaattctct tatcttgctt atccccatag
    39301 tattactagc ttcatatagt ctataatcgg ttgaacttag agatatataa agtatttgaa
    39361 atgattgagg aaatacatat ataatttgtg ccaatattat tcccactgct ccatatattg
    39421 gaacatgcac tccaagtaaa ttagttacca aaccttgatt tccaaataaa tatactagac
    39481 ctattgcatg catcattgtt ggagcaaaca taggaaacat agctaatgat ttaaaaaaac
    39541 ctttaaattt aatctttgtt ctagttaatg catatgcata tactaatgca agtgttagtg
    39601 atattacagt tgatataact gatacaaata ctgtattttt tagagaattt aatagagatt
    39661 cagaagccaa gtattcttga aagttttgta atcctacaaa gactccatct ttatctaaaa
    39721 aagcctgtac aaatagatat attataggtg ctaataaaaa tattactaaa gacataacta
    39781 atactgtaag catacatttt tttatatcta aattcatttt tgatttaaga cttatacttt
    39841 tagaacttat agcattatta ttcatacact tatgctcctt taacttttgg atactttaaa
    39901 tagtattttt cgtctatact tatttttaca aggtcacctt ctactaaatt taaattaacc
    39961 cactctttta tagaaacatc acttattatg aaattttcat ttaatttatc ttttatttca
    40021 actctcaata aattccctct aaactctata tttgatatta tcccttggta gttttctttt
    40081 gtcgatttct ctatttgaac atattctggt cttacagtta atatagaatc tccattatca
    40141 aaacagtttg tatctcctat aaattgagct gtaaataggt tttgaggatt ttggtatatt
    40201 tcttctggag tccctatttg cattatatct cctccattta gaatagctat tttatctgcc
    40261 atagttatag cttcttcttg gtcatgtgtt accattattg tcgttatatt aagttctttt
    40321 tgtaatcttt taatgtccat tctaagcttg tgtctaacct ttgcatctaa tgcagacatt
    40381 ggttcatcta aaagtaaaaa ctttggttca agagctaatg ctcttgctat tgcaatcctc
    40441 tgttgttgtc cacctgataa tgctttaggg tatttatgag cttcattagt caatccaact
    40501 gtttcaagta cttccttaac tttattatct attttttctt ttgaaacttt tctctctttt
    40561 agtggaaaag ctatattgtt ataagcagtc atattcggaa ataatgcata tgattgaaat
    40621 actataccaa aacctctctt tgatggctct aaatttgtaa tatctttatc ctggagaatt
    40681 atagttccac tatttacatc ttcaagacct gctattattc taagtaatgt agttttacca
    40741 catccactag gtccgagtaa acatagaaat tccccttctt caatatccaa actaatatta
    40801 tttaaaacct ttttttggtc ataactttta aacacattat ttatttttaa gtaactcata
    40861 tcttatctct ccttatatag ttattttttt attttaaatt ttatatccta ctttttcttc
    40921 taaaattctt tcatctaaat tattaattta aactttactt actttcagat tttgaaccgt
    40981 attttgattc ccaagtttta agtactgtac ttctgttttt ggcagcagag tttaaatcaa
    41041 cttcttttat aagctgttca aatggttgtt tagaataacc ttttggaact ggattatctg
    41101 tctttatgga agtaactgct acttcatgtg catatctatc catagctgaa tcacttattg
    41161 cccaatctaa aaatagcttt gcttcttttt ttatattatc ttttttaact aaggcatttg
    41221 cttctaagtc ccaacctgaa ccttcctttg gaaatacaac ctctataggt tctccactag
    41281 ataattgttg tgtagctcta tatccaagtg taatacctat tggatactct ccagagccag
    41341 ctaacttcgc aggttttgaa cccgagtgtg tatatgtggc tatattttta tgtaatttat
    41401 ccatgtaatt gtaagcatct ttttcaccca tcatttgaat aagccctgat actgtaagat
    41461 accccgttcc agatgatgct ggatttggca ttgttataag tcctttatat tctggtttta
    41521 ttaaatcttc ataacttttt ggaatatcta tacctagttt cttaagttcc ttataattaa
    41581 ctactattgc tgtctctggg acactcattc ctacccaaga tacatcatct tcagtatctt
    41641 taaatttttt atcaacttta tcataatttt taggcttata tgcttcaagt gcaccatctt
    41701 tttttagctc tatcatgttc attgctgata atccccatat aatatctgct tgtggattat
    41761 ctttttctgc taatagtttt gaagctatta caccatgaga atccataact atctctacat
    41821 ctatattggg atactcttct ctaaaacctt ccatataata agctatctgc tcttcctcta
    41881 gtgctgcata tattgtaagt ttattatttc cttttccttg agtagtttgt ggtgttgttg
    41941 atatgcaact tgtcataaat gtaatcacta caatgcacag acaagtaaat ataaattctt
    42001 tcatatcttc ccccctttgt gttttttcct ttttttattt gttttttatt gtttttgctt
    42061 atgtttatat aatacaccta atttgttaat ataaagttaa gtgtaaatta acagttcgtt
    42121 aatttttaga aatttttgtt aaatatatta tttttagtta aatacagtct gtttttaaac
    42181 aatatattaa tttttatagt atctacattt taatttaaac ataaaaaata agtctaacct
    42241 ctataaagta gaatgttaga cttattttta taagtattta attatacaac caaatcattt
    42301 tttttctttt cttcaagtgc ttttttaagc accatatcct taaccttcat aagtctagta
    42361 gtacttgctc tctcattttc ttcaagcttc atagctatat acttaatagt ctcaatgtaa
    42421 tttggtatca ttacattttc aagagcatta acacgtctac gtgtcttttc tatctcttgt
    42481 gcaagaagct gtgcactttt ttcactttct gcaagtctta aaagtggttg catagcatct
    42541 gagaaagctt ccatagcaga atctaattca cctgatgtaa aagcaagacc ataaggataa
    42601 atatcacttt gattgttttc agttttaaag tcaaacacag gaacatcaac actcattata
    42661 tttctcgtag aaacatctac tgaaactgat tgctttggca tcataagtgc tgaacccaga
    42721 tactcctgac tcataactgc tcttgcgatt ataaagtttt tataagcagt atcaagagca
    42781 ttttctgctt cttcacgcaa tcgcttattt tctcttacaa tctcaagaaa ttgtttcata
    42841 agttcatcaa gtttatcttt aagaagctta tgacctcttg tagcagtttt aaggagtttc
    42901 ttaagtctag tcatttccat acgtgttgga tttatattta atctagccac agatatcact
    42961 ccttaggcat atatttttca aaatattcat cacggatacg tttaagttca ctttttggta
    43021 acatactaag aagcttccat ccgatttcta atgtctgctc aatagttcta tcagttctaa
    43081 atccttgtga aacatattct ttctcaaatt catctgcaaa cttagcatac attaaatcaa
    43141 tctcagaaag tgcagattca ccaagtatag ccataagttc ttttgcatcc ttacctcttg
    43201 aataagctgc aaaaagctgg ttcattgtat ctgcatggtc ttcacgagtc ttacctttac
    43261 caataccttt atcttttaga cgagaaagtg atggtaaaac atctatagga ggctgtatat
    43321 cttttttata aagttcacgg ctaagtataa tttgcccttc tgtaatgtat cctgtaaggt
    43381 caggaattgg atgtgtctta tcatcctcag gcattgtaag tattggaatc atagttatag
    43441 acccttcatg ccctttcatt tttcctgctc tttcatacat agtagcaagg tcagtataaa
    43501 gatatcctgg atatccacga cgtcctggaa cttctttttt agctgctgaa acttcacgca
    43561 atgcctctgc atagtttgta atatccgtta ttataacaag tacatgcata tcttgctcga
    43621 aagcaaggta ttctgctgct gtaagtgcca ttctaggtgt agatacacgc tctacagctg
    43681 ggtcatttgc aaggtttaca aacactacag aacggtcgat tgcaccagtt gctttaaaat
    43741 cgcttataaa aaattcagca tcttcaaaag ttataccaac tgcagcaaat acaacggcga
    43801 acttagaatc agttccacgt acttttgcct gtcttgcaat ctgaactgca agattggcat
    43861 gtggcaaacc agaccctgag aatataggaa gtttttgccc tctaaccaat gtattaagtc
    43921 catctatagc agagactcca gtttgtataa actcggccgg ataatcacgt gctgctggat
    43981 ttattggagc tccatttata tcaagtcttt tatctggtat aatttctgga cctccatcaa
    44041 taggtctacc cattccacta aaaacacgac ctaatatatc tggtgaaact ccaaagtcaa
    44101 gactctttcc taaaaaacga actttacttt cagcaaggtt tatacctgtt gagctttcga
    44161 aaagctgaac aagtgcagta tctccattta cctctagaac tttgcaacgg cgaatttctc
    44221 catttgaaag ttcaatttct ccaagttcat caaattttac accttctacg ccgtttatta
    44281 acataagagg accggctacc tcttgtattg acttatattc ctttatcata gttcttcagc
    44341 ccccctcttt ttaataaggt ctgcaagctc gttgtctatt tcatcttcaa ttgaatcgta
    44401 agctgcatct atatcgcctt cttctacata cttagcacga ccaatctttt caagaactga
    44461 aagcttgata atatcgttta tagtaacatt ttgcttgata gcatcttgtg ctgacttata
    44521 gaattttaat ataagtccca tcattctata ttgtttatga agtgatgtat aagtatcaac
    44581 ttcatggaaa gagttttgat gaaggaagtc ttctcttata gaacgagtaa cctctagtat
    44641 caatctatct ccatctgaaa gtgaatcaac accaaccaat tgaactattt cttgaagttc
    44701 agattcaact tgtaaaagtt tcatagcttg agctctttga gctgagaaat tatcatttac
    44761 atttttatct gcccatacat caactttttc ctgatagagt gaataactag tcaaccagtt
    44821 aattgctggg aaatgacgtt tatatgctaa agatgcatca agaccccaga atacttttac
    44881 tatacgaaga gttgcttggc tgacaggttc tgatgtatca ccaccaggag gagaaactgc
    44941 accaattgct gtaagtgttc cttctctttc atccatacct agacaattta cctttcctgc
    45001 tctctcatag aattgagcaa gacgtgaacc taagtatgca ggataacctt cttcaccagg
    45061 catctcctct aaacgaccac tcatctctct aagagcctca gcccatcttg atgtagagtc
    45121 tgccataagt gcaacactat atcccatatc tctaaaatat tcagctattg taataccagt
    45181 gtatatagaa gcttcacgtg cagcaaccgg catatctgat gtatttgcta taagcacagt
    45241 tctttgcata agtgactcgc ctgttcttgg gtcttttaat tcaggaaatt caagaagaac
    45301 atctgtcatt tcatttccac gctcgccaca gcctatatat acaactatat ctgcatctgc
    45361 ccatttagca agctgatgct gtgtaacagt ttttccactt ccaaaaggtc ccggtatagc
    45421 tgctacccca ccttttgtga ttgggaaaaa tgtatctata actctttgac ctgtaacaag
    45481 aagtgaatct ggtgttttct tttctctata tggtctttct tttctaactg gccatttttg
    45541 catcatagtt acaggtatat cattaccttt ttcatctgta ataacacaaa ctgtatcttc
    45601 aacagtaaat tcaccagaat atatttcttt tatagtaccc ttaactccat aaggaaccat
    45661 aattttacat tctactactg ctgtttcttg aacgccacca attatatctc ctgctacaac
    45721 cttatcacca acagattttt taggtttaaa ttcccactta gttgttctat caagtgaaga
    45781 tacttcaaca cctttagtta tattagtacc tgaaatatca tacatttttt caagtggacg
    45841 ttgaatacca tcataaatag ttgaaataag acctggtcca agttcaacac tcataggcat
    45901 tccaagagaa actacttctt cccctggtcc tagaccggaa gtttcctcat aaacctgtat
    45961 agaagcttta tctccatgca tttctataat ctcgcctata agatgtttat ctgaaactct
    46021 tacgacgtca aacatattcg catctctcat gccctccgct acaacaagcg gtcctgacac
    46081 ctttacaatt gtgcctgttt tcatattctc tcacctgcaa tcatatactt tatcaaaatt
    46141 taaatattcc ctcctatatt ttattagcac atatatttag caccaataaa atttgagatt
    46201 gtcttttatt caactttcat ctattcttta aataatatat ctgcacctat agcacgttta
    46261 ctagattctc ttacttcatt catacctaat cccatacttc ctccaatacc tggaataact
    46321 ataatggcag gaagttggta atctttgtat tttgctattg attcttttgc aagcaatgat
    46381 gcctgttctg taatataaat tatcgcatat tcatcttcaa ccaacttatg tatacttttt
    46441 ttaatttcgt cactatcata ggctgggaag atatctattc caagagctaa aaatcccatg
    46501 atgctatctt tatctccaac tactcctacc ttatacataa gcatccctta gcctttcttt
    46561 tatagtatct gcgtctatat tattaagctt actagtcaaa ataattctaa ttgtttttat
    46621 ctctgattct ttagcatata aatatgcaat tagtggttct aatgtaagag gcttaaattt
    46681 tgctcctcta acatattcca taagatagtc atcacaaagt ttttcaaaga cagtaaaacc
    46741 actatccatg ccatttttac agacatcaga gtaagaagta gatttaaaac atgatgctgg
    46801 agtatctgag gaaaaagctt ctaagaattt ttccttatca agtgagccac ctgaaacaaa
    46861 cacattcata aacatatcaa aatctttctt catatttttg actcttataa aactctttag
    46921 atttgttaaa tcacaaactt tcataacata atctattaca aatttgtttt ggctttcttt
    46981 agcagtttct ttcatactgg taaaggttgc tttatctaga acaatatcaa ccatctggcc
    47041 attttgtgtc ttattataaa catcaaatgc ctctaatata gctgatgaca ttatcttagg
    47101 taaatcacta aaactacgat ttgctaaaga ttctctaagc ttaataagag gaattgtacc
    47161 tccatcaatt aaatatttct caccatctac accagaaatt tcttctttaa tcagcacttt
    47221 taaattgtga tagtcatttt tataaagaaa tacatcaaca aatttttctt caggagctag
    47281 acttttaaga atttcatatg tctttgataa ttcttgagtc aaaatattct caaaattatg
    47341 tatattatta tttgatgtat cagcataacc tgcctcagct ataattctta aagagtcttc
    47401 agggcttttg gcttctgcca tttgaataaa catttgtctg ttaagaagct gtttttcaag
    47461 cactcttatt cgtgcaacgg catagggata agttttttct tctgccattt tttaacccct
    47521 ccttaattaa acaaaatttc tgctgcaatt tgttcaatat attcatgctc tactgcaatt
    47581 atggcttcaa atgaataatt gtactcaatg tcaccttttt taactatgaa acctccagta
    47641 atatctctag tttcatcaga cactttaatg cctttttttg atagcacatc ttttaatgtt
    47701 tttttatctt ttttagataa tactatctct gaatcatctg tacaatttga tttttcaatc
    47761 atgtttagta taatctcttc atattcatta tctttaagat ttaaaagttt gtttttagct
    47821 tctaaaatta cctcttctaa aatctcttgt ttttttgata atatttgctt tttagcctcc
    47881 atataagctc ctgatatttc cttagaagcc gctttttctg cttctgcctc tgcaagcttt
    47941 gtataggaag ccatttcctt ttcagctttt tcattagctg aatttacttt taaatctgct
    48001 tcacttttag ctttatccaa tatttcttgt gcttcttgtt tggcatcagc tataattctg
    48061 tcaatcattt tttgttcatt acccatagga gtgcttcctc cttttcattt tttttactta
    48121 tgaattataa tttataatta accaattgca attccattgt aagctaagaa agaaacaagt
    48181 agtcctaaaa gagcatatgt ttcaactatt gctgcaaatg ttatagcttt accaagttct
    48241 tcaggtcttt ttgcaactat tccaactcct gctgctgctg ctttaccttg tgctatacct
    48301 gatactaaac caactaaacc aattggaagt cctgccataa gaagttgtaa accttgagct
    48361 gatgttggta ttgcatctcc accaataatc cctgcttttg ataaaataag gaaagctatg
    48421 ataaatccat acagaccttg tgtaccagga agaagttgta aaactagaag ttgaccgaat
    48481 ttactagggt cttctgttac aacacctgct gcggcttgac ctgctattga agtaccttta
    48541 gcagaaccca tacctgcaaa taatgctgct atagcagctc ctgatattgc caaaaattga
    48601 ccatttccta acgcgtttaa aaattccata tttacatctc ctttttatta atgcttgtat
    48661 attttgtttt atacttaaac ggaacaaaag gaactcctcc gccttcataa aacttattga
    48721 aaaattctac atactgtaac ctacttgtat gaacatatgc acctagtgca tttataagaa
    48781 ggtttattgt gtgtcctaca acaccgatta caactgcaag tatcggacca gctatagctc
    48841 ctaaaaggtt gataacttgt gctataactc ctgttgttaa acaaagcgcc ataattcttg
    48901 tataagataa tatatcagca aaataacttg ttattccata gagacttgaa aatcctttaa
    48961 aaagcttaac aggaatacct ttaaagcttc ttccttgagt aagtacaaga cctatagcac
    49021 ctataattgc taaatatttt cctacttctg aaaatacacc tatgtcccct gcaacaaaag
    49081 gtattataag tagacataca cctgttatac acaaatacca aaaacctatg tcaaagattg
    49141 catctattac ctttccactt cttattaatg tatatgcttt tattcccaaa cctacatata
    49201 tatggataat accgaacagt aacgacattc ccataagtgt cataacatcc tctaaaggat
    49261 taattaatgc ttttatagga attaaatccc ccaaaatacc cccgaatata agaccccata
    49321 cagtagttga aactccacag ataccaataa gtttaattaa atttccttca cctttttgta
    49381 tctttaattt atatacaaca aatccacaaa ctactgcaat tattaaccca tatgcggcgt
    49441 cacttagcat cattccaaag aatacaacaa agaagaaagt taaaattgta tttgggtcaa
    49501 tatcttttgt acttggatag ctgtacatgt tagttacact ttcaaaaggt gttactaatt
    49561 tattgttttc aagcagaatt gggaagcctt cttctttact tccttcttca gtctgaacac
    49621 aacaatcaaa cttttctgta atttctttaa ttaattcatc tgcccttttg cttggtaacc
    49681 aaccattaag acagaatgtt gtttttgttt tgacaagtct ctctataatc tttttttggt
    49741 ctctttctat agtgtaaaaa tcatataggt tttcaagcat gtgtatacta tctgcatgtc
    49801 ttttaatttc ctctgttttg tccttagaaa cgttttcctg tctaattatc atttcattgt
    49861 atttcttgat agcttgtaac ggtgtacctt cttcatttgg taatgaaacc actgaaaaat
    49921 taaattcttt taaaacttct aaaacatcat caaaagtttc ttttaaagta attaaataaa
    49981 catagttcat taacttgtca gaattgacaa tcccaataac actttctggt atttcatctt
    50041 ctaattttac tcttaaagca tcaatgtttg cgcttatggg cacagttcca agtatagttt
    50101 ttgtatactt agtttccata ttttcaatag gaatatcaaa gttcacccat ggttctaaca
    50161 tacttttatt gttgttaagt aagtttatgt tagaccttat tgtacctatc tccttgtgca
    50221 aattgtttaa ctccagagct tcagcataaa cttgcccttc tttttcacta tcaattttgt
    50281 taaactctct ttttggtgca agtagggatt ttttttgttt cactagtgaa gaaacaatat
    50341 taattgcttg gtttacatga gacattttct gctctagcat tataacttca gattcttcac
    50401 tatcttttac aagataattt tttaattcct cctcctcagt aagaaaggca ctatcatcaa
    50461 tctgaacaaa cccacggttc ataagtagct ttaaaatctg gcttttttga gcttctaagc
    50521 caacaataga tattttgttc atattaacta ttgccatatt atctcactac ctttccaata
    50581 accgcttcaa tagccttact ttttctttct tcagcagttt tttcaatttt cagacattca
    50641 cttttagcat tagctaagat tttatcaacc tctgctctac tgtcggtgac tgccttctca
    50701 ataagagttt cttccataag ctttgcttgt ttttttacat cagcaaatat gctttcacat
    50761 ttgtttacag catcagctct gatagactct gcatcagtaa cagcttttac tttgatactg
    50821 tctgctgtct gctctgcctc aacaatgctt tttattattt caaaagccat ccaaatcacc
    50881 tctcttacct ttttactatg tatataacca attagatttt tttattctaa ttagattact
    50941 acctcattgt taaagtataa acagcatttt tatataataa ttaatatttt tcaacgttta
    51001 tttcttaata aaacatattt tagaatagta cttgcctaga tttacatcct tttttgactt
    51061 acttttagac tatgacatga gtatggtttt taaataccat tattttcaat aattataatt
    51121 taaagcgata tagtttgtca atagcgttat tttgttttaa aattaacgtt atgtcgacat
    51181 tttaaatttt tattttattt tttttataat tttaatttaa tttattacat ataaaagttt
    51241 tttatattct ttttcaacct ctaaccattg aagtttattg ttttttttga atttttagaa
    51301 atatctcgca ctattttatc taacttttat aaatcatcaa gctatatcga tttttgtcac
    51361 cttattttta atctaatttt tttatatcta gttagaaata aatcaatatt tttaaagatt
    51421 gataaaactt acacctagtt ctaatagttt tattttaagg caatgtatta atcttaagta
    51481 gataatatca ttagttactc tatatattcg acaacattat aaaaaaatcc tcgtaccaat
    51541 cactccaatc ttcaatagga tatgctgctc ctccatttac acttttcagt agattggatg
    51601 tatattcttc tcccatagaa aaaagttccc ctactgtcat atcataacct ctagatgtaa
    51661 caaccttaca aaatgaatcc ataacatcac gttgttctct tgtttttata agttcctttc
    51721 taaattcatc atcattctta gcttcttcta aaattttagt taatttttca ttcattatgt
    51781 atttctcctt ttaagtagtc tttctgtaaa cttcaataag ctttatataa aaaggaagcc
    51841 atcctaaata aactaagatg acttcgctta actgttagca acacatttac tagtataaaa
    51901 taattggtgt acctggttct atattttcat atattttctt tgccttagaa aaaggtgtat
    51961 ttacacatcc atgtgaacct gaagacttat atatacttcc accaaaagaa tttctccaag
    52021 tagcatcatg aatgcctatg ttcccattaa atggaagcca ataacttaca ggtgaactgt
    52081 aaccttgacc agttaatgaa acatttctag ccttataatt aagaggatat atacctgatg
    52141 gtgtagcaaa tcctctagct aaatcacctg ttacaacgtc tgttgataca agcatttctc
    52201 catttttaaa gaaccacata tgttggccag aaagactaat ctctacatat gtatttccaa
    52261 tatcatcttt attacgagat acagcttttt gtatgtaaat aggttctctt gtaacatttt
    52321 taccagcttt tacaacatca actaatgctt cagcttcttt ctttttatct atcaaccaac
    52381 cataaattcc acctgaaatt gtaatatctt tacctgtggc agttttaaat ggtcttgagt
    52441 caccaaaagt actgtatttt ttacttaaac ctcttgtata ttctaccata gcatcattat
    52501 caaaagttat gtcataattc ttttctgagt ctacttttaa ccatttatta atttcagaac
    52561 catctaaagt ttccttacgg tcactaaaat catatgtaac gactacacca acatacttat
    52621 ctaatgtctt aatagcttgt ttcaactttt ctgaatcctt tgtgttgttt ggtgcttcat
    52681 aaactttact ttcatccaga gaaatagaag tattcccaga attaattgag ttttgtacta
    52741 acttataaaa atcatctttt ttcactttgt ttccaagtac ctctggtact ataacataag
    52801 cgtttttagt gctatcatac ttaggataag cactcttagg ttctacaaca tttttactat
    52861 caaagcaaga tagagcgtta aatctttttt ctaataaatc tttattatat gaaagattta
    52921 ccatattatt atactcttta gacttaaata ttccaccaat ccatccaata gcgctctgac
    52981 tatcttttat cttttgaact ttatcatctg gtgtaaattt taaatcaata tcttttgctt
    53041 ttatttgttc tttcttattg tttctttcct ctaagtttaa agtataggaa tttgtactag
    53101 atgttaacaa atcatttact tgctcaactg tttttcctga aacctttacg ccattaatag
    53161 tcgttccaaa gtaaaaatga tttctaaaaa agattgccat tcctatataa gctacaaaca
    53221 aaacacctac tactatagct atcttaaatt ttagattttt ttgtccactt tcttgtttta
    53281 cctcttttcc atcaatcata attaaatcct tccttacatt gtaattacct atgtacctta
    53341 ttggttttta aacaaaacca tgtatgcctt attttatata cccaaattac tgtatcatta
    53401 tttatatttt taaatatata gttatttcca taaaaccatt ttatctttta gataaaatga
    53461 ttattataaa tccccccttt caaaatccct taaacacaat ctctgctata ttaatatatt
    53521 accatacttt taataatcgt gggactatct atctattttt gtaacactaa actactcttt
    53581 taaaaaagaa actaattttg tgttagtttc tttatcttaa cttattttta tattgaattt
    53641 tagcttaaca taacgttgtt atatagattc tatttaatta ttttagtttc tatatctttt
    53701 ccatctgcat attctttaag taattcctta gcatatccaa aatattcttc aatatcagat
    53761 tcttgactaa aagaaaatat acaaattaat atttcatttg catcttctgt tatcattgct
    53821 ctttcagaat cactagttgg actcccaaat ttacccactg aatcagacaa tataggaaga
    53881 ttttctatat ttattaattc tttcccaatg cccttatact gttctccttc atctcctaca
    53941 gtaaaacata ctggtgaatg taatttgctt acattatatg tacctactgg atataatgat
    54001 tttattgata ttaaattgtt tatttctact atattattta ttgtataaag acccttccct
    54061 tgaataattc ttctaaccaa tgcttccgaa gatgttctat atctacttgg attttttcca
    54121 agctctttat aagcatttct tgcatcttta attttatcta gtttcgtaat gtcttcaact
    54181 tttagacttt gatttatagt atcacaaaac tcatttattt cattaattaa tttttctgag
    54241 cttttattta ctgctacttt tgcttgaatg caacccagct tagcattagg ccatctttcc
    54301 ttaagtttgt catcaatact tatatctatc atacttaaac aactcctata cactaaattt
    54361 ttattaaagt ttttatcatc tatacattat agtactattc ttttgttaat aacatgttta
    54421 ttagcaaaat aaatttattt ttaatataat ttttataaaa tacaattatt ttttgcttta
    54481 tattagacaa tacattgata aaataaaaaa acactaatta tttagcaatg atttattaaa
    54541 atcaagcatt cataatagtg ctttatataa aaatcaaatt ttgttttaaa gtaaatctaa
    54601 aaaattctta gctggcattg gtcgtgcaaa ataaaatcct tgaactaaat cacatccaaa
    54661 ttgtttcatt aactcagctt gttctttact ctcaacacct tctataatta caactaaatc
    54721 tagtgatttt gctagagaca ctattgattt tgctatattg cgacttctct catcatgttc
    54781 aatgccacca agaaaagact tatctagttt caatatatca attggcatat ctcttaaaca
    54841 actaatagaa gaatatcctg tgccaaagtc atccatagaa atagaaaatc catgcttcct
    54901 caattcattt aaaacattct ttaaaatatt taactcatta atagccacag tttctgttat
    54961 ctctatttca atgaaatctg aacttagatt atacatttgt attgttttat tgataaattt
    55021 tacaatatct ggttgataca actcgactct agataaattt actgaaactg gaactatctt
    55081 tttaccttgc tttttccatt cagataaatc attgcaaacc ctttcaaata caaatctccc
    55141 aatttttaaa atcaacttac ttttctctgc tattggaata aatactgcgg gacttataaa
    55201 accatgttct ggatgattcc atcttattag tgcttcactc cctatcattt ctccagttac
    55261 aagtgaaaac ttaggttgat aatacacttc aaattgattt tttaccaatg ctatttttat
    55321 atcatctaaa atcattgatt cttcactaag cttattccta acatcttcat tataaatagc
    55381 ataatttata tttttacctt tagcaacaga cctagccata tttgctttat ttacagcctt
    55441 ttgaatatct acttcacctt cttcaacaaa ataaataccc acacacagag agatttcaaa
    55501 gactatactc aaattacttt caatattact tcgtatatta tcaagttttc ttataatttt
    55561 atttcttcca tttttataat cacagaaaat tgcaaaataa tcacttgaaa gtcgtgcata
    55621 tacctcacct tctgttaatt cttcttttaa agcttttgct attgtaatta ataaattgtc
    55681 accaaattca tatccaaaag tatcattaat catcttaaag ttattaatat caaaatatac
    55741 tataatataa tttaaagatg gtttatctaa aagatactta ctacactctc tataaaattt
    55801 attttgattt cctatatatg taactttatc ttcataagcc aaacttaata tttctttttc
    55861 attagatttt tttatataaa ttatataaaa aataattgca ataaatatta atacaataga
    55921 taacactgca tatacagctc tgttgattat ttgttttgaa ttactaaata caacagtttc
    55981 tggaattgaa aaaattaaat tccagtcttt tatataatta ttttcactat tcccattatc
    56041 catagtggca tatcctaaat atcttctttc ttccaacata tttaatacag ttgcacctgt
    56101 tttattttct tgcaattctt tttttaatct atttacttct tgaatatcat tatcctgttc
    56161 taacaattta taaatatttt ttgataggct atccccttgt ggatgaagga taattgtacc
    56221 atcatttttt acaacataag atatactatt attttcataa aaagatatat taataagttt
    56281 catcagagta ttgatattgt aagtacaata taaaactcca actactttat cattatttaa
    56341 taatggtact gaaatagcaa tcttctttat agacttattt acttcatcaa catatggact
    56401 tgaaacatac ttcttacctt taattgagtt tttgaaaaat tctctatctt tcactgaaaa
    56461 cttatcacct ttggtatttt cagcattacc atttatatca actattgcca tagtgtaaaa
    56521 tttttgattt tccatttcat ctaaaagatt tttcattttt tcctcattag gtatatctgg
    56581 atttgaagca taatagttag aaaaaatatt taaaacatct aaatcttttt caatttgtct
    56641 ttgtgctagt tgagagcctt gtactgcaac ttcttttata tgtatttttg cctctgacat
    56701 aagaagatgt tttgtatctt ctatatagat ataccccatt gtcaaaaata atgatgttaa
    56761 aaaaagaatt gtaattaatg taaaactttt atttctccct gtcattcgac tcctccccta
    56821 attagtcaat acttcatata gataatatat cctttttata ccaattcatc atttatattg
    56881 tatataacta ttcttaaatg ttacacataa aatcaacata ataaccataa aaacattctt
    56941 ttattattat aataacacta tttaattgaa ttttaaatta aagaataaaa actatccatt
    57001 ttctcaaaca aaaaaactct tatacaataa aactttgttt tatgtataag agtttttatt
    57061 ttatctattt tatagcaaac ttatcttcca taatatgaat ttaaatacat atcttttatt
    57121 tcattcataa gtggatatct tggatttgct cctgtacatt ggtcatcaaa tgcttgaatt
    57181 accatttcat ctattgaatc cataaactta gattcttcaa ctccaaactc ttttatagat
    57241 ttaggaatac caactttaag ttttaaatct tcaatagcta ccaataaact ttggaatttc
    57301 tcttcatcag aatttccttt taatcctaaa aatgaagcta tttcagcata tctctctaat
    57361 gcttctggat atttatattg agagaatgct cccattttct ttggtgcatc tgaagaatta
    57421 aatttcataa cttctgttat caaaagagca tttgcaacac catgtggtac atggtggaaa
    57481 gcacctaatt tatgagccat agagtgacaa acacccaaaa atgcatttgc aaaagccata
    57541 cctgccattg tagaagcatt tgccattttt tctctagctt ctgggtcttt tgcaccatta
    57601 tcatatgcac gtggaagata ttcaaatata ctcttaatag cttgtaaagc taatccattt
    57661 gtataatcgg ttgcaagcat agatacatat gcttctaatg catgagttaa agcatctata
    57721 ccagaagcag atgttaagct ttttggcata ttcatcatca tatcagaatc aactatagcc
    57781 atatttggca tcagttcata atctgctaat ggatatttta ctcctgtatc ttggtcagtt
    57841 ataacagcaa atggtgttac ttctgaacca gtacctgctg atgttggtat agctacaaaa
    57901 ttagcttttt cacccatttt agggaatgta tatacccttt ttcttatatc cataaatctc
    57961 atagctaggt cttggaaatc tacatctgga tgctcataca atgtccacat tatctttcct
    58021 gcatccattg cacttcctcc acctatagat attattacat ctggattaaa ttctctcata
    58081 gctttagcgc ctatcttagc acattccaaa gttgggtctg gttctacttc aaagaatgta
    58141 gtgtgcttaa tattcatttc atctaataaa tcagtaactg ctttagtata tccattatta
    58201 taaaggaatg tatctgttac aataaaagct ctcttcttat tcattacatc tttaagttct
    58261 tttaatgcta ctcctaaaca accttttttg aaataaactt tttctggtgc tctaaaccaa
    58321 agcatatttt ctctcctctc agctactgtt ttaatattaa gtaaatgctt aactcctaca
    58381 ttttcagata ctgaatttcc accccaagac ccacatccta aagtaagaga aggagctagt
    58441 ttaaaattat ataagtcccc tataccacct tgagaagaag gagtattaat taaaattcta
    58501 caagttttca tggctgaagt aaacttatca agtttttctc tttgatttac atcatcaata
    58561 tataatgaag atgtatgccc aaatccacca tcttctacta atttttctgc ttttctaact
    58621 gcatcatcaa aatcacttgc cttgtacatt gcaagtactg gtgaaagttt ctcatgagca
    58681 aaagcttctt ctatttcaac tgattctact tcacctatta aaaccttagt atctactggg
    58741 acttcaaagc ctgcaagttt tgctatagta caagctgttt gcccaactat tttagaattt
    58801 aatcctccat ttactaaaat tatatttctt attttttctg tttcatcttt atctaataaa
    58861 taagcaccac gctctttaaa ttcttttttg acttcattgt atatattttc taatactata
    58921 accgattgtt cagaagcaca tatcatacca ttgtcaaaag tcttagatac aagaattgag
    58981 tttacagcag tttttatatc tgcactatca tcaattattg ctggagtatt acctgcacca
    59041 actccaatag ctggttttcc agatgaatac gctgatttaa ccattccagg acctcctgta
    59101 gcaagggtca aatccacttc tttcataaca gttgtagtta attctaaaga tggctcatct
    59161 atccatccta ttataccttc aggagctcca gctaaaacag cagcctcaag aacaactttt
    59221 gcagcctcta tagtagaatt ttttgctctt ggatgtggag agaaaattat tccatttcta
    59281 gtttttaaag ctaaaagtgc tttaaagata gcagtagaag ttggattagt tgtaggtaca
    59341 actgctgcaa taacacctat tggctcagca attttagtca taccaaatgc ttcgtctttc
    59401 tctataacac cacaagtttt agtttcttta tatgcattat atatatactc tgaggcataa
    59461 tgattcttta taaccttatc ttcagcaatt cccataccag tttctttttg tgccattata
    59521 gctagaggaa ctctttgctt attagctgcc aaagatgctg ctaaaaaaat tttatctacc
    59581 tgctcttgac tatatgtagc aaatattttt tgagcttctc tgatttttgc tagtttttta
    59641 actaagcttt caacgttatt tacagtatct aaaccaactg acataacttc attttctttt
    59701 ttattttcta ttattacttg ttctttatga gccattttaa caacctcctg agatagtttt
    59761 ttatttatta tgttaattta ttaacattat agcatttact ttttctattt tcaagttatt
    59821 tttttaacat tttatttttt atctaatata acaccatttc tactaataat atctagcttt
    59881 acaaatactt aatttaacta gatatcactt tattttttac gcaaatcact aatgaaatca
    59941 tctaaaaact taaggataac gttatttttt taacgtttat atttttgtat tttttacaaa
    60001 taaaacttta taaaaagaat ttttaatatg atactctatc aagtttaaaa ataaaaatac
    60061 ttgaaaatga aaataaaaac ttaattaaca agttaaag
//
LOCUS       DAELJO010000005        59798 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELJO010000005 DAELJO010000000
VERSION     DAELJO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.005"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(6..773)
                     /locus_tag="KPB88_000245"
     CDS             complement(6..773)
                     /locus_tag="KPB88_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052559.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNSENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(803..1912)
                     /gene="ugpC"
                     /locus_tag="KPB88_000246"
     CDS             complement(803..1912)
                     /gene="ugpC"
                     /locus_tag="KPB88_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG0052560.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            2170..2907
                     /gene="cas6"
                     /locus_tag="KPB88_000247"
     CDS             2170..2907
                     /gene="cas6"
                     /locus_tag="KPB88_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0052561.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2912..4387
                     /locus_tag="KPB88_000248"
     CDS             2912..4387
                     /locus_tag="KPB88_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052562.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLMYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLKNMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            4407..5420
                     /locus_tag="KPB88_000249"
     CDS             4407..5420
                     /locus_tag="KPB88_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG0052563.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            5436..6233
                     /gene="cas5"
                     /locus_tag="KPB88_000250"
     CDS             5436..6233
                     /gene="cas5"
                     /locus_tag="KPB88_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0052564.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            6260..8668
                     /locus_tag="KPB88_000251"
     CDS             6260..8668
                     /locus_tag="KPB88_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0052565.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            9110..10057
                     /gene="prmA"
                     /locus_tag="KPB88_000252"
     CDS             9110..10057
                     /gene="prmA"
                     /locus_tag="KPB88_000252"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG0052566.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            10076..10834
                     /locus_tag="KPB88_000253"
     CDS             10076..10834
                     /locus_tag="KPB88_000253"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG0052567.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            10836..12134
                     /gene="mtaB"
                     /locus_tag="KPB88_000254"
     CDS             10836..12134
                     /gene="mtaB"
                     /locus_tag="KPB88_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG0052568.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            12177..12527
                     /locus_tag="KPB88_000255"
     CDS             12177..12527
                     /locus_tag="KPB88_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG0052569.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            12690..12869
                     /locus_tag="KPB88_000256"
     CDS             12690..12869
                     /locus_tag="KPB88_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG0052570.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            12899..13342
                     /locus_tag="KPB88_000257"
     CDS             12899..13342
                     /locus_tag="KPB88_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG0052571.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            13453..13950
                     /locus_tag="KPB88_000258"
     CDS             13453..13950
                     /locus_tag="KPB88_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG0052572.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            14143..14706
                     /gene="raiA"
                     /locus_tag="KPB88_000259"
     CDS             14143..14706
                     /gene="raiA"
                     /locus_tag="KPB88_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0052573.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            14919..15155
                     /locus_tag="KPB88_000260"
     CDS             14919..15155
                     /locus_tag="KPB88_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG0052574.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            15168..16511
                     /gene="yqfD"
                     /locus_tag="KPB88_000261"
     CDS             15168..16511
                     /gene="yqfD"
                     /locus_tag="KPB88_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG0052575.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            16450..17565
                     /locus_tag="KPB88_000262"
     CDS             16450..17565
                     /locus_tag="KPB88_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG0052576.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            17622..18083
                     /gene="ybeY"
                     /locus_tag="KPB88_000263"
     CDS             17622..18083
                     /gene="ybeY"
                     /locus_tag="KPB88_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG0052577.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            18089..18808
                     /locus_tag="KPB88_000264"
     CDS             18089..18808
                     /locus_tag="KPB88_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG0052578.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            18927..19328
                     /locus_tag="KPB88_000265"
     CDS             18927..19328
                     /locus_tag="KPB88_000265"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0052579.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            19410..20303
                     /gene="era"
                     /locus_tag="KPB88_000266"
     CDS             19410..20303
                     /gene="era"
                     /locus_tag="KPB88_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG0052580.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            20315..21694
                     /gene="mgtE"
                     /locus_tag="KPB88_000267"
     CDS             20315..21694
                     /gene="mgtE"
                     /locus_tag="KPB88_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG0052581.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            21767..21922
                     /locus_tag="KPB88_000268"
     CDS             21767..21922
                     /locus_tag="KPB88_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG0052582.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            21888..22661
                     /gene="recO"
                     /locus_tag="KPB88_000269"
     CDS             21888..22661
                     /gene="recO"
                     /locus_tag="KPB88_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG0052583.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNFVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            22654..23253
                     /locus_tag="KPB88_000270"
     CDS             22654..23253
                     /locus_tag="KPB88_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG0052584.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            23413..24291
                     /gene="glyQ"
                     /locus_tag="KPB88_000271"
     CDS             23413..24291
                     /gene="glyQ"
                     /locus_tag="KPB88_000271"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG0052585.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            24291..26357
                     /locus_tag="KPB88_000272"
     CDS             24291..26357
                     /locus_tag="KPB88_000272"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG0052586.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMSFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(26523..28070)
                     /locus_tag="KPB88_000273"
     CDS             complement(26523..28070)
                     /locus_tag="KPB88_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG0052587.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            28350..29117
                     /locus_tag="KPB88_000274"
     CDS             28350..29117
                     /locus_tag="KPB88_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0052588.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            29400..29597
                     /locus_tag="KPB88_000275"
     CDS             29400..29597
                     /locus_tag="KPB88_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0052589.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            29626..30702
                     /gene="vorB"
                     /locus_tag="KPB88_000276"
     CDS             29626..30702
                     /gene="vorB"
                     /locus_tag="KPB88_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0052590.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            30705..31424
                     /locus_tag="KPB88_000277"
     CDS             30705..31424
                     /locus_tag="KPB88_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0052591.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            31437..31976
                     /locus_tag="KPB88_000278"
     CDS             31437..31976
                     /locus_tag="KPB88_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0052592.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            32133..33191
                     /gene="buk"
                     /locus_tag="KPB88_000279"
     CDS             32133..33191
                     /gene="buk"
                     /locus_tag="KPB88_000279"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0052593.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            33209..34102
                     /locus_tag="KPB88_000280"
     CDS             33209..34102
                     /locus_tag="KPB88_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0052594.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            34132..35340
                     /locus_tag="KPB88_000281"
     CDS             34132..35340
                     /locus_tag="KPB88_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0052595.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            35364..36623
                     /locus_tag="KPB88_000282"
     CDS             35364..36623
                     /locus_tag="KPB88_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0052596.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            37268..38731
                     /locus_tag="KPB88_000283"
     CDS             37268..38731
                     /locus_tag="KPB88_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0052597.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHYGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            38787..39923
                     /locus_tag="KPB88_000284"
     CDS             38787..39923
                     /locus_tag="KPB88_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0052598.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            40159..41274
                     /locus_tag="KPB88_000285"
     CDS             40159..41274
                     /locus_tag="KPB88_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG0052599.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMSLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            41426..42295
                     /locus_tag="KPB88_000286"
     CDS             41426..42295
                     /locus_tag="KPB88_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0052600.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            42671..43216
                     /locus_tag="KPB88_000287"
     CDS             42671..43216
                     /locus_tag="KPB88_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0052601.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            43229..44242
                     /locus_tag="KPB88_000288"
     CDS             43229..44242
                     /locus_tag="KPB88_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0052602.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            44464..46437
                     /locus_tag="KPB88_000289"
     CDS             44464..46437
                     /locus_tag="KPB88_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0052603.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLKEGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            46506..46865
                     /locus_tag="KPB88_000290"
     CDS             46506..46865
                     /locus_tag="KPB88_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052604.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            46900..47256
                     /locus_tag="KPB88_000291"
     CDS             46900..47256
                     /locus_tag="KPB88_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0052605.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            47293..48585
                     /locus_tag="KPB88_000292"
     CDS             47293..48585
                     /locus_tag="KPB88_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0052606.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            49021..49659
                     /locus_tag="KPB88_000293"
     CDS             49021..49659
                     /locus_tag="KPB88_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052607.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            49666..50508
                     /locus_tag="KPB88_000294"
     CDS             49666..50508
                     /locus_tag="KPB88_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG0052608.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            50569..53196
                     /locus_tag="KPB88_000295"
     CDS             50569..53196
                     /locus_tag="KPB88_000295"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0052609.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            53410..53655
                     /locus_tag="KPB88_000296"
     CDS             53410..53655
                     /locus_tag="KPB88_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0052610.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(53944..55242)
                     /locus_tag="KPB88_000297"
     CDS             complement(53944..55242)
                     /locus_tag="KPB88_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG0052611.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(55276..58410)
                     /locus_tag="KPB88_000298"
     CDS             complement(55276..58410)
                     /locus_tag="KPB88_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG0052612.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(58414..59637)
                     /locus_tag="KPB88_000299"
     CDS             complement(58414..59637)
                     /locus_tag="KPB88_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0052613.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISNPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
BASE COUNT        23209 a         6584 c        10657 g        19348 t
ORIGIN      
        1 agtagttaaa tttttttata gagaaaataa gatttgacac ttagataaaa aatcattgca
       61 ttttcaaaat tctttaaatc aaacccaagt atctcattaa tcttttccaa tctatatact
      121 agcgtgtttc tatgtaaaaa catactagta gatgtcttag aaatatttaa atcattattt
      181 ataaatttta ttcctgtata tagaagttct tcgtcaatag atttaatctt attaaaatct
      241 attaatttat tgagtatatt atcctcatta attaaatcta aagttttata tctaataagg
      301 tcaaacaaat ttaaaaactt attttcagag ttgtctttta taatatgagg tagtaaatca
      361 gaacataact gtaatctaaa atcaaaatca gctttactat ctaatttacc aagataaaaa
      421 atataagtac tttgataagt ttcagtttca attatatttt gtgtagttac attaatatct
      481 aagttatatt tagaatttac tataaaaaaa ccattgtgtt gtattatatt tatatcttca
      541 aataaattat aaaaaatatt ttttagatat tcaaagtcta tatgagattg gtaaattaaa
      601 taaaagttgc cttttaaatt atcaagatta tttttatcta gaaaatcaat aacataaata
      661 gcattattga aagtaactaa atggctacta gcatctaact tttggttttc atatggatag
      721 atatttattt ttttattaag ctgtctttct aaaaaatctt ttaactcttc cataaagtct
      781 ccttaaaaat aagattatat tcttatctta tagctaaagt agtttctttg tcaaatatgt
      841 gtactctact attgtcaact gaaagtgtta agtcatcacc aacattaatt ctgtttttac
      901 tgtcaaatct aattgttatc gtattttcgc ctagttttaa gtgagcataa gtttctgctc
      961 ccataagttc ttttatttca attcttgatt taaaacaagt atctggatta gatttaataa
     1021 aagtatcttc tacatgaata tcttctggtc tgatacctat aacaacttca ttattaatat
     1081 aattgtcttt tattaaatca tagtgttttt ctttattcaa tttaagctta atatgttcat
     1141 tttgtgcata aatttcatca ttttcttcta ttaatattac atcaataaaa ttcatctgtg
     1201 gtgctccaat aaatcctgca acaaatatat tagctggata atcgtataca tcttgtggag
     1261 ttgcaatctg ctgaactact ccatccttca ttactacgat tctatcagcc atagtcatag
     1321 cttcaacttg gtcgtgtgtt acatagataa atgtagttcc aagctgttgg tgtagcttag
     1381 ttatctctgt tctcatagca gttctaagtt ttgcatctaa gttggataga ggttcatcca
     1441 ttaagaatac cttaggattt ctaactattg cacgtcctaa ggcaaccctt tgtctttgtc
     1501 ctccagataa tgttttaggt tttttattta aaagaggtag taagtctaat attttagcag
     1561 cttctttaac ttttttgtct atttcatctt taggcaattt tctaagttta agtgcaaatg
     1621 ccatattttc gtaaacagat aagtggggat ataaagcata gttttgaaaa accatagcta
     1681 tatctctatc ttttggttct atattattga caagtttatc tccaatgtat agttctcctt
     1741 cagagatttc ttcaagacca gcaatcattc taagtgtagt cgatttacca caaccagatg
     1801 gtcctactaa aacgataaat tctttatctt taatgtctat atttatattt tttactgcgt
     1861 taaatccatt tgagtaaagt tttgatatat tttttaaaat tacttctgac ataatatcct
     1921 ccttgatatt taaattttaa ttatatagta tcaaattttt ttattatatt aaatgtgtaa
     1981 aatgactaaa tgtatttgtt taatatgtat atttagtttt tagcaaagaa taaaactaac
     2041 agtagaatat atttaaaaaa ttaaaaaaca aaattaaatt tattatcaaa aaatcatttt
     2101 atgatatgtt ttatttttat attacaaata gagaataatg aattatacta gaaagtagga
     2161 ggtgaaaata tgagcagaat ttcaattttt atggaatcat gtaatggcaa atttccaaat
     2221 gaaaattcaa tgttgtcagt ttcctttata aaaaatattt taaatgcaga aaacaaagat
     2281 tttgctaaaa acatatttaa ctatggggaa gaaagaaaaa acaataagca aataaaggat
     2341 ataaatacag ccatttacat accaaatctt ataagaagca aaaatgagtt acttgttgat
     2401 ggagatataa tattcaatat aagtttttat aattattcta tgttcagtaa attatacaat
     2461 ggaattttaa aagtaaagga gctagaatat aaaggttata gatttaaaat aaaaaatata
     2521 aaaatacata aagaacacga gataaaagaa aatggtgtta tatttaaaac tatgtctcct
     2581 ataatagtaa agaataaaga aggtaaatat ttagatgtag aagattcaaa ttatatagaa
     2641 tgtttaaact atatagctaa tctaactctg gagagtataa gaggtagtgg gcttagaaag
     2701 ccattggaat ttatacctat taacttcaaa aaaagagtgt taaaagaaaa gattcgtgga
     2761 tttaaagtga gagaatatta ctatataaat gcttatgcag gaacattttt tttaaaagga
     2821 gatatggaag atttaaatgc actgtataaa atgggaattg gatatagaag gacagaaaat
     2881 gcaggaatgg ttgatatatt aaagtagcga ggtgagagaa ataaaagcta aattttatag
     2941 atcaggtgat attgtaagag atattggcat tgtttacttt tatgaattac taatggattt
     3001 aaaaaacgaa ctagaaaaaa atgattataa gctaaaattt acttgtgagt taaatagaaa
     3061 ttatttatct ttagaatctg cagaatatat agaccctaag tatatatctg attatatatt
     3121 agaaaatcaa ctttttaagg tatttaaaaa tgggaaaaag gaaaccttag aaaaaatatt
     3181 tagtaatata gataatttaa tgtatgtaaa ttttattgaa gaattggata aatcttctgc
     3241 aactgctaaa caaaaggaag atattaaaaa agattttaaa aacaggaggt ttccatatgt
     3301 tagaaatagt ggtaagtatg gattaaacac gagtttaaaa aatatggaaa caaatgtaaa
     3361 aagaatagta gatactgtaa ttaaatcaaa tatagagata gatgaaatag ataaacttga
     3421 tttgacacaa tatgaagaaa aagatagtgt atgtaatgtt tgtaatataa ataaaacaac
     3481 aaaactagat attgatttaa gagagcgaaa ggactctaag tataactttt tatttagagg
     3541 tgcagaaaaa tcaggcttta aaagaagtgg acaagttgaa agtaatattt gttttgagtg
     3601 tgaatttttc aacttaatgt gtcttttata tataaattta aaaagaccaa tggtttttgc
     3661 atatacgaat gatttaaggg aattagcatt tttaaatcat aagataatgc ttaaaagaaa
     3721 gatgtattca gataaatcat tttataaaaa acttttgcat gaaaaaataa gttcaattag
     3781 actatatagg tttgatattg atacgaataa aggtataatt ctaaagtttg attctattat
     3841 agagtataaa gaattattaa aaaaaattga acttatagat attatagata attataattt
     3901 ttcaagagag ccaggtaata ctagaaattt gggtaaaaga atgattgata atgggaattt
     3961 atcaaattta aaagaattat tacttgataa tataagtgtt ttaaagcaat caactggaac
     4021 ttctcgagaa atggattttg tatctagctc atataatatc ggtttatata taaaattgtg
     4081 ttggattgta gatggagggg aggagtataa aatgaaaaac tatatggaaa gtaatttaat
     4141 atattcaaga gtaggtaaag acttatttaa taaaatgaca gatgaatcca gaagaaaaaa
     4201 tttttctatg aaagtgattc aactacttaa gtctaatgat agaactcagc tatttcaaac
     4261 tataatgcat gtgttagttt ctaatggaat cttaatagga gaagggtttg tagaaggtct
     4321 aatgcagagt tctgaattag aattaaatac gaatgtaggt ctatttattc aagaaatcat
     4381 gaaataaaaa agggggaaaa tttaaaatga atgaaaaaag agtaaaagga gtaacattaa
     4441 ctatactgac agaaagccca gttccacttt catatgacca agggtatgga aattatacac
     4501 caataaaaaa agaacaatat agagaaaaaa tacatgcaaa aactagtata gccacaataa
     4561 catatgattt aagaagaatg ctccaccaag aatatggttg gaatttaagt aatattgttt
     4621 ttggaaaaaa aggaaatata tatccatcta ttaagaaaaa tgtaggatgt gtagatgaaa
     4681 atggattaga gactgatgta tttggttatt taataccact taaagataaa ggttctataa
     4741 gtaaagcttc tccacttaga ataatacctt ttaggtcatt aaatccatat aaaggttcaa
     4801 cacagctaat aacaaataga ggctttttaa gtagtgaatt tggtagaaaa tattatgatg
     4861 agaaggaaga aaatgaagta cctagagatg aaaactttcc aacatcacaa gcattagcaa
     4921 tagaagagac tttatcagat tactacgttt atacaataac attggagctt gatagaatag
     4981 gagtagtaga agtagaagat ggaaagttac ttttaccaga agaaagaaaa tttatgagta
     5041 aggagttaag agaaaaagca gtaaaagata tattagatgc aattactgtg tttacaagaa
     5101 acataaaaca ttcatcagta ctattaaaac cacttgcagt aataggtgga gcttttgata
     5161 aagttgtacc atttttttgg gatgatgtag attataatgc tgatagtggt gagataaatt
     5221 tagagggagt catagaaact atagaaagtt atagccttaa agaaagtaac acaatacttg
     5281 ctatcaatga tagattaaaa atttctaata aaaaggaatt aaataagttt aatttaagta
     5341 aataccctgt aaaagagatt aaaaatctag cagatagatt ggaaattgga gaagataata
     5401 tgtggtattt aaaagagtag gaggtttttt gaattatgaa agtattacaa tgtaaactta
     5461 agcaaccaac tgctcactat agagacccaa aagtatttca aaatgaatat ataagtactt
     5521 taaatttacc atctaagacc acaataatgg gaatgataac gtatttatgt gatagaagat
     5581 taaactctga tatagatata ggaataattg gaacacatca tcatagagaa ctagaatttt
     5641 caagaggaga aaacattgat ttttggaatg aatatataaa aatgggaaaa ggtaaagata
     5701 aagaaaagtt tttgcttaaa ggaaactact atgattacta taaagagcat aaagctcaaa
     5761 atagcatatt aaactatgaa gttcttaaag aagtagaact gactatattt atttcttgca
     5821 aggatgatga agaacttgaa tttattagaa aaaagcttga aagtccatgt aaatatgcaa
     5881 atttaggaag aaaagaagat tttgtaatac caagtgaaaa agggtgtttt gtgaaagaag
     5941 ttaagttaaa agaggttata cctatgaata ctcgtgatgc tataaaagaa aatataaaat
     6001 taaagaatac atatgtaaga gttgacttaa gagataaaga gaatgtcgag actataataa
     6061 atcaaggtgt attgattgca ttgcctcata aatataaaga tttagaagca aatagagatg
     6121 atagagttta tgagttttgc cattatatat atgttgataa tgatggtata tatcctaaaa
     6181 atattaaggt aaatgtatgt attgatacaa aagaagtttt tacatggctt taaattattt
     6241 tgaaagatag gtgtttatca tgaaaaatat catgcaaaaa gaaatattag ctaagtcata
     6301 caatttaaga actaatcaac aagagcaaac attagttgaa catattaagg atttatttga
     6361 agtattagaa tctatattag aattaaatct atattcagat aaagatgtag agatactaaa
     6421 aatatgttgt gctttgcacg atttaggaaa aattaattca attatgcaac aaaaaatgga
     6481 gattaataat aaaatatcgt atagttgctc agaagaagag aggaaaaagt tagagtctga
     6541 caaaaaatct ttaaaaaaga tagcaagaca taatatattt tctggagcat ttttaaaaga
     6601 tatattagaa aaaatgaact tgtcagaaga agataagtta tatatatata aaagtataat
     6661 gttacatcat ggaaactatg aagattatat gaggttaagt attagtaaag ttcaagaaga
     6721 aatttatgag tatattgaaa aaggcatttt agaaaaagaa gagtttaatc tgaaagatat
     6781 agaaagttat ataaataaca ttttaaatgt agactttaaa tttggtgaag atgtattgga
     6841 ttatgattac atagataaat taagtgaaag tatgtttata gacagtgatt ataacaatgg
     6901 tcaaatagat gattcagttt taaattatag aaagtttaag tatattttat ataaagggat
     6961 gctgaactta atagaccata gtgcatcaag tagacagaaa ggtataaaat tttataacga
     7021 ttttactgat gaagaaattg ataatatgat tttaaatgag atatataaga gtcagggtaa
     7081 tttagaaaaa aataatatag agttcaatac tatacaaaaa aggttgagag aactttctgg
     7141 aagaaatgtt ctgactgttg catttacagg ttcaggaaaa acagctgcag attatagaaa
     7201 gacttttaaa agaaaattct ttttagtgcc aaataaaata tcagcagaaa gtttttatag
     7261 aaaaaatatt tttcaaaata aaaatgattt agatacaaga agtagtaatg attacattgg
     7321 attacttcat ggagatataa acttatattc agagaatgaa gataatggag aacatgattt
     7381 tgtattgaca ttaagggata tagatttgag tataaacttt tgtaaacctt gtgtaattgc
     7441 aacagttgat cagttattac ttagtatgtt taaatttccg ggatatgaaa aaatattcgc
     7501 agccgtaaaa aatgcatcta taactgttga tgaagtacat ttattggaac caaagatgtt
     7561 tttgattttg atatatttta tgcagtttgc atgcaagtac ttagatgttg attttcactt
     7621 aatgacagct acacttccaa aatcatataa agagcaaatg ataaacaagg gaattatttt
     7681 tcaagaagaa agtaatgaaa atgttactga taaaggagaa atagttttta ttgagagcaa
     7741 caaggaagaa gagatttgtg aaggtaaaga tgtaaaggtt tcttttataa aggaaaaaga
     7801 aatcaagtca atagttgaag atgctttaga aaataaacaa aaaattctta taataaagaa
     7861 tactattgat tcagtcaata gaacatatga gtttttaaaa gaaaatttat ctgataagta
     7921 tggtggtgta gatatagatg ttttacatag tagatttaag ttcaaagata aaaaagagaa
     7981 atatagtaaa atattaaatg gagagggtga tatttggata tcaactcaat cagtagaaat
     8041 atccttggat ttggatttca atataataat aagtgattta gcaactatgg atagcttaat
     8101 acaaagaatg ggtagatgca atagaaataa taagtatgaa tatggaaact tttatattct
     8161 acctagtgaa gataaaattt atgacaacaa acttaagagc acaactaaaa acatcttaaa
     8221 ggacatatta aaaactaact ctatttttac aatgggaatt agaaaaacta ttttggatga
     8281 ttactatgat aatagtgtag tcaaaaaata ttttgaagag aattttataa gttgtgataa
     8341 agaaattaag aatatttatg gtgtaaataa agaaattttt gatggattgg atttaatttt
     8401 taattttgaa ccttacaaaa atattgttga tagtaagaag gaagcagcta aaatttttag
     8461 agatgttgat gttagttaca aaattattct ggaagaagat ttttataagg aagatagaga
     8521 gttacaacaa gattcgattc aagtatcagg gtttatattt aatagattat attattatgg
     8581 gttaataagc aaagtagaag gatacatggt actaaaatct agtagtaagt ttgagtataa
     8641 tagtactgta ggcttaattt tgaagtgagc atatgtttaa atataatata cctttagaca
     8701 ttgaaaatac aaaaagtact actgtaagct cactgtaaaa actatttttt taaaaggaaa
     8761 aataagttgt gagatagagt gtttacaagt gtatatagat aataaatttg aggtttaaga
     8821 ttaactaagt gggtttcagg tacatcgcga aaataattat aagttggaga ttttgtatgt
     8881 ttaagattaa ctaagtggat ttcaaatatt gatatagtag atataataga agttttatta
     8941 ttaggtttaa aattaactaa tcggattttt aaatattctg actattatat aaggatatta
     9001 ttagcttgaa tttaaaacca ataatataaa acaacaaaat attatattta tgatataata
     9061 aaagttatgg aaaaacagga atattactat agagaagaga ggtaaactta tgaataattg
     9121 gatagaagta actataaaaa caacaacaga agcagttgaa cctataacga atatactata
     9181 tgaacaagga gctggaggag ctgtaataga agacccaaaa gactttttat tccaaaagaa
     9241 aaatgaacta gactgggatt atgttgaaga agaagtattt aaaaagaatg aagaagatga
     9301 tgttcttata aaaacttatg tttctgaaga aaaaaatgtg atggaatttg tcgaaataat
     9361 aaagcaaaaa gttttagggt taaaagattt tggtatagat ataggtgaag gaagtgtatc
     9421 attggaccaa gtaaatgaag ctgattgggc aaatgcatgg aaagcatact acaaaccaac
     9481 aaaagtagga caaagggtag tagtaaagcc tacatgggaa gattatgcta tgcaagaggg
     9541 ggatttaatc atagagcttg accctggaat ggcatttggt acaggtacac atgagactac
     9601 tagtatgtgc attagagaat tagaaaagta cgtgaataaa gattctaaag tatttgatat
     9661 tggctgtggt agtggaatcc ttgcaatagc agcagctaaa ttaggagcaa aagaagttgt
     9721 agctgtagat ttagatgaag tggctgtaaa agttgcaaaa gaaaatgttc ttgaaaataa
     9781 agttgaaaaa agtgtatctg taatgcatgg taatttaact gatgtaataa aagataaagc
     9841 ggatgtaata gttgcaaata taatagctga tataataaag atacttgcaa aagatgttca
     9901 aaactttatg aaagaagatg ctatattcat atcttcaggt ataattttgg ataaagtaga
     9961 agaagttaaa gaaagcttaa ttgaaaatgg atttgaaata gttgaagttc aaaaattagg
    10021 tgaatggtca gcaatagttt ctaagcttaa aaaataaggg atatggtgac gtataatgga
    10081 tagatttttt gttgaaaaaa ataatataaa tctacaagat aaaacttgca ctatagaagg
    10141 tgaagatgta aaacatattt caaaagtttt aagatgcaaa ttaggtgaga aacttgaaat
    10201 atgtgacaag aataataatg agtatatttg tgagataatg aatatagata agagtatagt
    10261 taatttagaa attttagaaa aagttgatat aaatagagaa tcagaactta aagtaaggct
    10321 ataccaggga cttccaaaag ctccaaaaat ggaaatgata cttcaaaaat taactgaagt
    10381 gggtgtagaa gaaataatat tagttcaaac taagagaagt gttgttaaag ttgatgacaa
    10441 aaaagaagat aaaaaatttg aacgttggga aagaataatt tatgaagcag caaagcaaag
    10501 caaaagaggt aagataccaa aattaagagg tgttttaagt tttaaagaag ctttagaaga
    10561 tatgaaaaaa aataatgtaa atatttgccc ttatgaaaat gaaagaacag tttcaataaa
    10621 acatgcacta aaaaaatgtg attctaatat agatagtgtt ggaattttta taggtccaga
    10681 aggtggattt tctgaagaag aaatagaaca aatacaaaaa aataattgta atgttgtatc
    10741 attggggcct agaatattaa gaactgagac agcttctgtt gtagcatcaa caatagcttt
    10801 gtatgaacta agtgatttag gaggagaaaa ataaattgaa aaaagtagct ttttatacac
    10861 ttgggtgtaa agttaatcaa tatgaaacag aagctatgct agaactattt gagaaagatg
    10921 gttatgaaca agtcaatagt gaagagtatg ctgatgttta tgttataaat acatgtacag
    10981 ttactcatat gagtgataga aaatctcgtc aatatataag acgagttaaa aagaagaatc
    11041 cagatgcaat aatagccgtt gtaggatgtt attctcaggt atctccggaa gaaattttgg
    11101 atatagaaga agtaaattta gtcatgggta caaatgatag aagaaagatt gttgaagaaa
    11161 ttaaaaagat aaattcaagt aagaaagtta gtacagttga tgacataatg aaagttaaag
    11221 cttttgaaga aatagaaatc agccaaacta atggaaagac tagagcattt atgaaaatac
    11281 aagatggatg tgacagattt tgtacttatt gtatcattcc ttatgcccga ggacgcgtta
    11341 gaagtagaga tattgatagt atagtcgatg aagttaaaaa attggctaat aatggctata
    11401 aagaggttgt attaacagga atacatgttg cctcttatgg aaaagattta aaggataggg
    11461 atattaaact tcttgatgta ataaaacaaa taaaccagat agaaaaaata gaaagaatta
    11521 ggcttagttc tgttgaacca attttattta cagatgaatt tgtaaatgaa gttttaaaga
    11581 tggataaagt ttgtcctcat taccatttat cacttcaaag tggatgtgat gaaactctaa
    11641 aaagaatgaa tagacgatac acaactttag agtacaaaac tattgtagat agattgagaa
    11701 gtaaaatgcc agatgtggca ataactacag atgtaatagt aggttttcct ggagagacaa
    11761 atgaagagtt taaaaaaacg tacgagtttt taaaagaaat agagctttca caaatgcata
    11821 tttttaaata ttcaccaaga aaaggaactc cagctgcaac tatggaaaat caagttgacc
    11881 ctcaaatgaa acactttaga agtgaacaac ttttaaattt aagtaaagta aattttaata
    11941 aatttgcaac taaatttatc ggccgtgagt tggatgtttt atttgaacaa aatatagaag
    12001 gcaataaata tgagggatta acttcaaatt atataagagt tgttgttgaa agtgacaaaa
    12061 atatacaagg tcagatatta aaagtgaaaa taaatgatgt aaaagatgaa tatgtagaag
    12121 gaattttgct ataacatgta gaaattataa tatttataag ataggaggtg gaaagaatgg
    12181 attgtatttt ttgtaaaata gcgaacggag aaataccttc aactaaagtt tatgaagatg
    12241 atagagtctt agcttttaat gacttgaacc cagttgctcc atatcatata ttagttgtac
    12301 ctaaaaaaca ttatgatagt ttaattgata ttcctgataa agagatggac attgtgtcac
    12361 atattcatgt tgttattaat aaaatagcta aagagaaagg ttttgaccaa actggattca
    12421 gagtcataaa taattgtgga agtgatggag ggcaagaagt taaacatttg cactatcata
    12481 ttttagctgg taaaaagctt cctaattacg aagcgggaca aaactaaact aaattattga
    12541 aaaattgagt tccttcttga taaaaatact tgataaaagt aaaaatttag tttataataa
    12601 acaaagtgtt aggttgtaat gcatattatg caaaaagaac atagttacag tcccagcata
    12661 attgcgctat tggagggagg gaaaaagaaa tgtcagaagt aagagttaga gagaatgaaa
    12721 cattagacag cgctttaaga agattcaagc gtcaatgtgc aatgtctggc atcatgtcag
    12781 aagtgagaaa aagagagcac tatgataagc caagcgttaa gcgtaagaaa aaagctgaag
    12841 cagcaagaag aaaaaacgct aagaaatagt agtaaataag aataaagagg tgaaggggat
    12901 gtcccttaag caaaagttac aagaggattt gaaatcttcg atgaaaaaca aagatacagt
    12961 gagaaaatct gtagtaactt taataagagc ttctataaag cagtatgagg ttgataatag
    13021 ggtcgagctt gatgaagatg gaattatcga tgtaatagca aaacaattaa agcaacgtag
    13081 agatgcatta gttgaatttg aaaaagctgg aagagaagac ttaataaaag aaacagaagg
    13141 tgaaattgaa gttttaaaag agtacctacc tcaacaatta agcgaagaag agttagaaga
    13201 aattgtaaaa tctactatat ctgaagtagg ggcaacttct atgaaggata tgggaaaaat
    13261 aatgtcagtt atccaaccaa aagttaaagg aagagctgac ggaaaactta taaataagtt
    13321 ggtaaaacaa aacttacaat agattgatat agcctagaga gaaatctcta ggctttttat
    13381 agtttttggg aataacgagt catatttgag catatctata atcaaagaaa attttgaaag
    13441 aaggggaaaa atttgattat atcagtaaaa gagattagga attttttgat aaacatattt
    13501 ataccactaa taataggtta cttaagtagc atcttaacaa tgattatcag tggaacagat
    13561 atttcaacct attatttaca actcacaaag ccaagctttg caccaccagc ttttatcttt
    13621 cctatagttt ggactatatt gtacatattg atgggtatat cttcatacct aatattaaga
    13681 aagggttaca acttacctaa agtaagagat gcaatttttt attatggatt acaacttgta
    13741 ttaaacttta tttggagtat actatttttt ggatttggac ttagatttac agctttgata
    13801 gatataataa taatgatttt ggtttctata ataatgattt cgaagttttc taaaatagat
    13861 aagagatctg gatacataaa tttgatttat ttaatgtggc ttgtttatgc aggatttttg
    13921 aactatttta tatggtttat taataaatag aatgtaaaga ttttgtaaat taccctgtac
    13981 ttttaataat atcataacaa acgaaaaata aatagactat taaaaataat cattttcata
    14041 tacaataata ttaatttaaa tacaataaat ttataaaaat tgtatttaaa gattaacata
    14101 ttaaagtatt agaattggaa aggcttgaag ggagaggttt ttatgcaaat aatagtatct
    14161 gggagacaaa tgaaattaac tgacggaata aaaggatacg tagatggaaa attaagtagg
    14221 ttagaaaaat accttgatcc tgaatcagaa gtgaaagtaa cagtaagtgc gaaaaaggat
    14281 agacaaaaag tggaggttac aataattcct attaacggtc aaataataag agcagaagac
    14341 gtagaagatg acttatatgc agcaattgat attgtttgtg acaaattaag tagacaagtt
    14401 gtcaaatata aaactaaagt taaagacaaa gttcaaaata ataagagcat aagatttgaa
    14461 aatcttgact ttatagataa tagcagtgaa tttgatgatt atgattatga tgatgaagaa
    14521 gatgaaaata tagtaataga aagaagaaag aaatttaatg taaaacctat gagttctgag
    14581 gaggcgatac ttcaaatgga actagtagga cacaatttct atatgtttag aaaccaagat
    14641 aattttgaaa taaacatagt gtataagcgt aaagctggtg gatatggttt aatagaacaa
    14701 gattaataaa aattgtatac gtttagattt tatataagaa aaaataaaaa ggtaatcatg
    14761 ttgatttttt atcaatatga ttaccttttt cttgtttaaa taatatatta gatattaggt
    14821 atttattata taaaaatttt aaaactaatc tgttacaaca tcaataaagt gcataaatac
    14881 ctatttcaag cataaaatta attaaggggg taatgcttat gttagaaata tccactgatt
    14941 tacaagtaaa tcaaccagtt attactgtta caagtaatac gtttattagt atagaaaatt
    15001 atctatcaat attagaatat gaagttgatt taataagaat aaagactaaa gttaaaacaa
    15061 ttaaaatatc tggggataaa ctttcactta agtatattac tgatagtgag ataggtataa
    15121 aagggattat ctacaatgtt gagtatgttg attaggagga gatacacttg ataagtttta
    15181 taaggggata ctatgttata gttgtggaag gagtaggact cgaacaattt ttaaatcatc
    15241 ttataagaaa tgggataagt gtttacaatg taactagaat aaaaaatact aaaatggagt
    15301 ttcatataga tagacaagat ataaaagaat ttaagaatgt ttatagagga agtaagtttg
    15361 atataaaggt aaagcaaaaa actggggtcc cttttattat caaaagagtt tataagcaca
    15421 aaggtatgtg gatttgtgct cttgtttctt tatttttatt gatgtctaca tcacagtttg
    15481 tgacagatgt atatatacaa tctcctgaag gtataaaaaa agaagctctt agaaatgagc
    15541 tttataaggt tggagtaaga ccaggagtct ataagaagag tattgataga aaagaagtta
    15601 gagaccatat gatgtcaaaa tttaatgatg tggcatattt atctatcaat gtaaaaggaa
    15661 caaatatatt tgttactgtt acaaaaaagg cagaatccct aaaatctaca gaccaatcaa
    15721 attattgtaa tgtaattgct ttaaaaaatg gtataataga aaaggtaatc cctagaagtg
    15781 gtaaatcagt tattaaatct ggagacatag tccaaaaggg agatgtctta ttaaatggag
    15841 ctaataccaa gtcaatacca gaagtatggg caagtacttt ttatgagtcc acaaaaaagg
    15901 caagttatgt agatactgta aataaaaaaa ctggagaaaa gaaaaatata tatacactaa
    15961 gtttttatga taaagagttt acaatgagaa aaaatataaa atataaagat tatgttgttg
    16021 aaaataaaga aaaaaaacta tcaataggaa actatacttt tccaataaag ataaaaacga
    16081 gtacttttta tgaaacaaaa aaggttgaag taaaaaggaa taaagaagag ttaaaaaaag
    16141 aattatctga aaaagcttta aaagagttag aatacataat cccagcttcg gctagaataa
    16201 tagatgtaaa acataacttt aaggttaata aaaatatgtt agaatattta ataacagttc
    16261 aaactagtga aaatattgct aaaatttatc ctcttagtaa gtcagaagca gaaaggttta
    16321 taaaggaaga aagtaaacca gatgaaggtg aagaggaagt tccttccaat ccagaaaaaa
    16381 gaccattaaa tgatataaga aatgagtttg atgaggataa taaggataat aaagatcaaa
    16441 acaattctga tgaaaataat agtaatcaaa atagtaataa tagtcaaaat aataatagca
    16501 ataacaacta ataaaattaa acttaaaatc ttaggaggac taattcaatt gataatacaa
    16561 aagaaattta caatagcaga tggtaacttt gaaagagagt tatttggcaa ctttgatgaa
    16621 aatgtaaaat taatagagaa gactttaaat atagatgtaa tacttagaga aggcaatata
    16681 attttaattg gagaagaaaa aaatgtagat tctgcattaa aacttatgaa tgaattacac
    16741 caaactgtat caaatggtaa gcatttagat aagcagagta tttcttattc attatctctt
    16801 ttgcttgaag gaagtgaaca aaaaataaaa gaacttgagg ggacaatcgt cataactcaa
    16861 aggggaaaag ctgttcaacc taaaacttta ggtcaaaaag aatatataaa acttatagaa
    16921 aataatgata taacttttgg tgttggacct gctggaacag gaaagactta tcttgcggtt
    16981 gctatggcag taaaagcttt taaaagagat gaagttagta gaattatact tacaagacca
    17041 gctgtggaag caggagagag tctaggtttt ttaccaggag atttaaagga caaagttgac
    17101 ccatatttaa gaccactata tgatgcctta tttgaaatgt tgggtgctga taaatttaat
    17161 aagtatttag aaagaggtac tattgaggtt gcaccacttg catttatgag aggtagaaca
    17221 cttgataatt catttataat attggatgaa gctcaaaata ctacttctga acaaatgaaa
    17281 atgtttttaa ctagacttgg atttggctct aaagctgtag ttacaggaga tgtaactcaa
    17341 acagatttac cacaaaataa gaagagtggt ctaatacaag ctacagagat tcttaaaggt
    17401 gttccaggta ttgggtctat aatgctaaca gatagagatg ttgtaagaca tgagttggta
    17461 cagagaataa taagagctta tgagaagcac gacaaaagag aagaatttaa aaaagaagaa
    17521 cgtaaaaaag cgaaaatgca ggaaaagact ttcaaaagaa agtaaaagtg tagtaatata
    17581 gatatatagc attattaatg aataaaaagt aggtgtagtg aatggatttg atactagatg
    17641 atagacaaga taaattagaa gtaagtgagg agttaattga aaagataaag gatataatca
    17701 tagaatgttt agattatgaa ggatatgatg ataattatga agtgagtctt tcttttgtag
    17761 ataataaaga gattcatgaa ttaaatagag aatatagagg agttgataga gtgactgatg
    17821 ttttatcatt tcctctttta agtgatgatt ttgaggatgt tgagttagaa gaagaatccc
    17881 taggtgacat agtagtatct cttgaaagag ccttagaaca gagtatagag tataaccata
    17941 gttttgaaag agaagtatgt tttttaatat gtcatagtat gtttcatctt ttaggttatg
    18001 accacgatac agatgaaaat acaaaagaaa tgagagaaaa agaagaacac atattaaata
    18061 agttaaacat aacaagggag tagtttttat gaaaccagaa aaaactcgtc aaggtataat
    18121 taaagcattt aatgcagcta tagaagggat attgtataca tttaaatttg aaagaaatat
    18181 gaaaatacat tatttaggtt cagttgctgt tcttataata agtttgtttt ttaacttttc
    18241 aaaattagaa atgataatgt tacttatgtc catatgctta gttgtagttg ctgaaatgtt
    18301 taatacagct atagaaaaag ctgttgattt agtaactgat gaatatcatg ttcttgctaa
    18361 gatagcaaaa gatgttgcag ctggaggcgt tctagttgca gcactaaatt cagtagtagt
    18421 tggatatatt ttgttttatg ataaattgac agatatatct ggaatattaa tttataaaat
    18481 aagagaatca gaattacata taaccttgat atgtatatta ttggttctta tagctgttgt
    18541 agttgtaaaa gcattgactt ctacaggtac tcctctaaaa ggaggaatgc caagtggtca
    18601 tgctgcctta gcatttgcta tagcaactgc aattacactt atgactgaaa gagttgttgc
    18661 ctctactctt gcatatataa tggctgtatt agttgcccaa agtagaattg aaggaaaaat
    18721 acataccttt tgggagacta ttgctggagc tttgttaggt gtgttgatag ctatattagt
    18781 atttcagctt ggaatgttct ataattaaaa taaatgtaca taaaatattt aaaataaaac
    18841 aaatctgtgc taattaaatt tttatgatat aatgtagagt attaaataaa aataactttt
    18901 aattattgta tacaggagga gttgttgtgg ataacataga attgttaagg ttggcagaag
    18961 atgccagaca acattcttat gctccttact caggttttag agtaggtgca gctcttttaa
    19021 ctaagagtgg taaagtttat actggatgta atatagaatg tgcatcatta ggtggaacaa
    19081 attgtgcaga aagaacagct atttttaaag caatatcaga gggtgataag gatatctata
    19141 aaatagctat agcaagtgat aactcagaaa ataatgagca aacatatcca tgtggaatat
    19201 gcaggcaggt aattatagag tttggttctg atataaaaat aataacggga tatactaaag
    19261 gagaggtgtt tgagcatagt ataaaggatt tgttgccaaa ctatttctct ggaaatgatt
    19321 taaaataaat ttaaaagtat aaataaattt aaaagtataa ataaatttaa gtaattaaaa
    19381 caagaagata aaaagttagg agacagttaa tgttcaaatc aggttttgtc agtatagtgg
    19441 gtagaccaaa tgtaggaaaa tctactttaa tgaataatgt agttggagaa aaaatagcta
    19501 taatgagtga taagcctcaa actacaagaa atacaataca agcagtatat acagatgaag
    19561 aaatgcagat agtattctta gatacaccag gtattcacaa accaaaaaat aaattaggtg
    19621 aatttatggt aaaggcagca acagaagctt ttaaaaatgt agatttaata ttatttgtag
    19681 ttgatgattc taaaaaaata ggtcctggag atagaaagat aatagaggat ttaagaagtg
    19741 ttaagacacc aataatttta gttgtaaaca aaatagacca actagaccaa aaagatgagc
    19801 tatttgatat aatcaaaatg tatgatagag aaggtatttt taaagaaata gtaccaatat
    19861 cagcattaaa aggaaagaat acggatacct taataaaagt tatacaaaat tatttagaag
    19921 aagggccaaa gtattttcca gattatatga ttacagatca gccagaaaga gtattaatag
    19981 cagaacttat aagagaaaaa gttcttcact atcttaatga tgaaatacct catggtgtag
    20041 ctgttgaaat tgaaaaaatg aaagcgagaa atgataagga aatagtagat gtatctgctg
    20101 taatatactg tgaaagagat tcacataagg gaataataat aggaaaaaat ggtaggaaac
    20161 taaaaggaat aggtaagtca gcaagacaag atatagaatt gcttttaggt tcacaaataa
    20221 atcttcaatt atgggttaaa gtcaaagaaa attggagaaa tttacaaaat tatataaata
    20281 attttggata taatgataaa taaaggtggg ttatatggat aggaaactgg atgcttccca
    20341 agatttatta tatgaagtaa aatcattaat tgataataat aaagtattgg agttaagaga
    20401 attaatagag gaatatcata taattgatat attcgatata atggaaaatt tagaggaaga
    20461 catgaaaatt caattatttg aagtacttcc tttagatatg gcatcttcta tattagaaga
    20521 aggtagtgta gagtttttta ttagtatttt atctaaactg gatgtagaac actcgaaaaa
    20581 tatacttgaa cttatgtctc ttggagatat ggcagacaag ttgagtgaac ttgaagaaga
    20641 agaaagagaa cacataataa atcttttgaa tcaagagaat gccgattatg taaaagagtt
    20701 actattctat gatgaagatt ctgctggtgg aacaatgacc actggatata tttctataaa
    20761 taaagatatg acagctcttg aggctataga ccatatgaga gaagaagctg aagaagctga
    20821 aactatttat tatatatatg tggttgatga tgaagaaaaa ctagttggag tattgtcatt
    20881 aagagaatta ataatagcta gagacgcaaa tatagtagaa gatttgatga gtgaaaatat
    20941 aatctctgtt tatgtagatg aagatagaga agaagcagta agattagtat ctaaatacaa
    21001 tttaatagca attccagtag tagacagaca agaaaaatta aaaggaataa taacagtaga
    21061 tgatataatt gatgtaatgg aagaagaagc aactgaagat atgtataaat ttgcaggaag
    21121 ttcagaacat gaaagagaag ttgctgaaaa agaaaaccct acattgagag agcaaataat
    21181 atcagcactt agaggacgat taccttggtt aattattacc ttagttggag ggttgttggc
    21241 ttcgttaata ctttctaact tagactatat aatgaatcct gtatatgctc cattagtatt
    21301 ttttatacct gtagtaattg gtatgggtgg aaatattgga actcaatcat catctgttac
    21361 agtaataaca ttatctaata aggatttaaa ttttagcaat gtggttagag aaggtattgt
    21421 aggtattatt acaggtcttt tgtgtagtat aataacaggt attgtaattt actttgttat
    21481 gagaaagtta gacattgtat taattgtttc aatatcatta tttataaata tggttttagg
    21541 tgcaacaata ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag acccatctac
    21601 agtttcatca ccaattatat ctacagctct tgatataact ggtatagcag tttactttat
    21661 aataacaacg gcattattgt caaaaattgt ttaataactt tatgtatgat ttatcctctt
    21721 cttactaaaa atataagtat aaatgtaaga agggggtttt ttaaaaatga acaatatcag
    21781 ttgggaagtt tttaaaaaaa caggtagtat agatgcatat ttatatttct gtgattgcaa
    21841 aaacatacat gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg ataatcctga
    21901 acacccaggg gatagtactt aaagctataa ggtataagga aagtgacata atacttacac
    21961 tatttacaag aaagcttgga aaagtatctg caatagcaaa aggagctaaa aaaaataaga
    22021 gctctttgct ttcatcatca cagttatttt cttatagcaa ttttacctta aaaaaacaag
    22081 gtaatatgta caaggttact caaagtgaaa taataaaaag tttttataat atatcctatg
    22141 atatagaggc attttcttat gctacatata ttacaaaatt agttgaaaat tctatacttg
    22201 aaaatcagac taataatagg ctatttatat tacttgcgca gactctgtat ttgtatacac
    22261 aggataatac agacaatagg tttataacag cagcattcga gttaaaattt ttagactata
    22321 taggatttaa acctatagta aataaatgca ttaattgtga atgtaaaatt ttacaaaatt
    22381 ttgtgtttaa tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata
    22441 ataatataaa attagactta acaactataa gtcttatgga atatgtatta agaaatgata
    22501 ttttaacatg tagtaaagct aaagtatcaa aatatataac acatgaactt gagaatattc
    22561 tagacaagta tttgaaagtt tatgttgata atattaattt taaatcatta cacgtattgc
    22621 aaagtgtaaa aaataataag ggagtggata atgatgagta gtaatataac tattgaaatg
    22681 gttgattcag tctttgaaag agttccagga gcaacttatg cagaagcaaa agaagcatta
    22741 gtaatgtgca atggagatgt aattgaggca gtaatatatt tagaaaataa aaaaaatact
    22801 tgtaattgta aagcaaaatc tgctaaagaa actatggaag aagtatttgg caaagatgga
    22861 gaaaatataa agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt aagaataata
    22921 gttgaaaaag atggaaaaac tatgatgaac attcctttaa cagttggagt tgttggatta
    22981 gcttttgtac cattagctac tttagttgga ttatcagctg ctgttataag taaatatcgt
    23041 ataaaagttc aaaatgaaga agatggagaa attgtagatt taggtgaatt gaatgaagaa
    23101 aaacttcacg ttttgaagaa tatgataact aatgcagcaa aagatgtgaa ggatgtagta
    23161 gttgacaaaa aagaagatga caaagatgta actgctgacc ttatgaaaga agaatctgaa
    23221 aagaataaag aagacgaaga cgaaaataaa tagatataaa gtgactttta aaaaatgaag
    23281 agaagataat tataagaatt atcactaaca ttgacaaatt ttatagaatt tgatattcta
    23341 aaagttgtag aagaactatt aaaatagcct ttcgtgtaga cgagaggctt tattgttata
    23401 ggagggattt ttatgaattt tcaagagatg atactggcgt tgcaaaaata ctggtcaaag
    23461 caaggttgta tcatgatgca accttatgat attgaaaaag gtgcaggaac tatgaatcct
    23521 aatacttttt taagatcatt aggacctgag ccttggcaag tatgctatgt agagccttca
    23581 agaagaccag ccgatggaag atatggtgaa aacccaaaca gactatatca acatcatcaa
    23641 ttccaagtta tactaaaacc ttctccagat aatatacaag aattatattt ggaaagttta
    23701 aaagagattg gaattgaccc aagtgagcat gacataagat ttgttgaaga taactgggaa
    23761 gctgctacag ttggtgcttg gggacttgga tgggaagttt ggctagatgg tatggaaata
    23821 actcaattta cgtattttca acaagtaggt aatattgaat gtgagcttga gacaggagaa
    23881 attacatatg gattagaaag attagctatg tacattcaag aagttgatag tgtttatgat
    23941 ttaaaatgga atgataaaat aacttatggt gaagtattta ataaagctga gtatgaaaac
    24001 tcaatgtacg catttgaatt atgtgatgca gatatgttgt ttaatctgtt tgatatatat
    24061 gaaaaagaag cattaagact tatggaaaat ggacttgtaa taccttcata tgactatgtg
    24121 ttaaaatgct ctcatgcatt taatacattg gatgcaagag gtgcaatagg agttagtcaa
    24181 agagcttcat ttataggtag agttagaaat atggcaaaga ctgttgcaga aacttttgta
    24241 aaacaaagag aagaaatggg attccctctt ttaaaggacg gtgacaaata atgaaaaatt
    24301 atcttttatt tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc agtacactag
    24361 aacaattaaa aaacaatctt agtaaattat ttgatgaaaa tagaatagct tttgataaaa
    24421 taaatgcgta tgggacacca agaagactta ctttagtagt cgaaggattg agtgatagac
    24481 aaaatgattt agaagaggaa ataaagggtc ctgctaagaa aattgctgtt gatgcagaaa
    24541 ataatttaac aaaaccagca ttaggattta taaaaagcaa aggattaagc gaatctgaca
    24601 tatattttaa acaagttgga aaagaagaat atatatttgc gactataaag caagatggaa
    24661 aagaaacttc agaagtacta aaagacatat taccagagac tataaaatct gtagtattcc
    24721 caaaagctat gcgttgggga ggaaaaaatc ttaaatttgc aagacctata agatggatag
    24781 ttacattgtt aaatgataaa gtattagagt ttgatttaga aggaataata tcttctaatg
    24841 taacaaaagg tcatagattt ttaggtgaaa gtacaataga agttgattct atagaagatt
    24901 attttgaaaa attagaaaaa aattatatag ttttagacca aaacaagaga aaagaaatga
    24961 taagaaaaca atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag
    25021 atttacttga agaggtaact tatttagttg aatatccaac tgctttttat ggagaatttg
    25081 atgtggatta tataaaacta ccaaaagaag ttgttataac tcctatgaaa caacatcaaa
    25141 gatattttcc agtacttaaa gaaggaaaac tgttacctaa ctttatagct gttagaaatg
    25201 gtgattctta cagaatagac aatgttaaag ctggaaatga aaaagtttta gaagcaagac
    25261 ttgcagatgc tttatttttc tatagagaag atactaagag aaacttagaa agctacatag
    25321 aaaaattaaa aactgttgta ttccaagtaa aactaggaac tatttacgac aaaactttaa
    25381 gacttgaaaa attaagtgct gatatacttg atagtttagg tttagtagat gaaaaggcag
    25441 atactataag ggctgctaaa ttatctaagg cagatttagt tactggaatg gtaggagaat
    25501 ttgatgaatt acaaggtttt atgggaaaag aatacgcaaa agttggagga gagaatgatg
    25561 ttgtaagtga agctattttt gaacattatc ttccaagatt tgcaggagat atacttccta
    25621 agactaatgc aggggttgcc ttatcaatag ctgataagtt ggattcaata gcaggtttct
    25681 ttgctatagg aatacaacca acaggttctc aagacccata tgctttaaga agacaagctt
    25741 taggtatact tagtatactt atggacagaa aagttgctgt ggatataaaa gtacttattg
    25801 aacatgcttt agataattat tctgaattag attttaataa agaagaagtt gttgagcaaa
    25861 taatgagttt cttcaatgag agagtaaaaa atctatttaa agatttagga ataagatatg
    25921 atgttgttga tgctgtttta agttctgata taaatgatgt ttctgatatg catatgagag
    25981 ctttagagct taataattgg ctagaaaaag atgaattagt ggagatgctt actgcattta
    26041 atagagtttc tactctagct caaaaagcag aaagtgataa aattgatgaa tctttattaa
    26101 aagaagatgc tgaaattaaa ctttacaatg aatttaaaca agtaaaggta aaagttaatg
    26161 aattattaaa ggataagaaa tatggtattg ctttagatga atttgcatct ttaagaccag
    26221 tagttgataa tttatttgat acagttatgg ttatggataa tgatgaagct attaagaaca
    26281 atagacttgc tttacttaaa caagtatatg atactatgct agaaatttgt gatttatcta
    26341 aaattgttta taaataaaat ttaaactatt tataagaaat aaggctgtct caaaatagag
    26401 atagtcttat ttttcattgt tgagatgatg aaaatatatt tgatttcata gaaaaagagt
    26461 gtctcataaa ctaaaaagtt cattttgaga cacccatgtt ttttgtaagt aaatataata
    26521 aattatattt tatacttatc agttattttg tttaaaatat tatcatttga atttacccat
    26581 tcataaaaat tgcctgatga tgcgtccgat attttataaa gcatttcagg aatatcacta
    26641 gctttaaaat ctccaagtga ttttcccaat ctagtcttac ctatttcaaa actgcctcca
    26701 acaaataatt caaatgcatc tgtagatata ccatcaacat tcttcttttt accacataaa
    26761 cctatgctac caacttgatg aacaccacat gaatttaaac atcctgagat atataactta
    26821 ggtgctttat ttaaaatttc ttcattattt tgtaatctaa aataatctat gatttcatgt
    26881 aacattttct gactattttg tatgcccatt tgacaaattg gaacacctat gcaagatata
    26941 ctttgttcta attgagaatt acaactaata gttttactta tttctaatat tctttttgct
    27001 tcatttccat ttaaatttaa aatatataat ccctctgtca ttcctagtct aatcatagga
    27061 ttttcaacat tatctaattc tttaagtaaa gtacttaaat cctttgttaa caaaatacct
    27121 ccaactggat gtatatatac agtgtataaa ccatcctgtt tttgaggtat tagtctagaa
    27181 tcctgtatat ctattttaac tccaggttta gagtaatcta tttcttcaat attgagttca
    27241 agactaccat tttctttttc taatttatag tattctttaa atttttctaa gaatagttct
    27301 tcaccaagtg attcgaccat atatctaacc ctagctctgt ttttattttc ataatttcca
    27361 taatccataa agaatttaat cataccttca acacaatata aggcatcttt cggctttata
    27421 agttcatcaa gttctaaagc aactttgggt tgtttaccta gacctccacc taagaaaact
    27481 ctgaaatatg gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg tatactacaa
    27541 tgagcagtat ctgtgtaaca attagaataa gaaactttta attttctagg taaatggtat
    27601 gtagtagcat ttttgataaa atatttatca gtagcaactg catatggagt aacatcaaag
    27661 acttcactag ggtcaactcc agataatgga gataatccga catttctagg ataattacca
    27721 ccaccacctc tagtgaatat atcatttttt attccttctt ccatgatatc aacaattgaa
    27781 tttatagata aatcgtgtaa ttgtatagct tgtctagttg ttaagtgtat cttatctaag
    27841 ttgtgtttaa tagctatttc atagatttta ttaagttgat actgagataa aactccagca
    27901 gatactctaa gtcttatcat aaaagatttt ttgtctcttt gagcataaac accatatcca
    27961 ccagaaaatc ccttatattg cattttattt atttctccat ttaaaaattt tgaagaatat
    28021 tctttaaaat taggtagttc atttaacaat atctttttaa aattatccat tatttcacct
    28081 cattgttagt cttgtataaa aatatatcta ttgtataata gatatatata ttattatcta
    28141 gtcaggataa aaatataaca gaataattct attaaaagta agaataataa ggacatagtg
    28201 agtattttgt ttttatttta caaattaagt ataatatttt tgttttataa aatataatta
    28261 agataaatag ataattttgt aattttaata tataatattt agtagagtgt gggagtagca
    28321 cttaatattt gcaggaggag tttttgtata tgggagaaat aataaatgca ttagatagag
    28381 ccctagacat aattttgctg ttatatcatg aaaagagaga aatgggaata actgaaataa
    28441 gcaaggctat gggagtatat aaaagtactg tacatagaac tttggttact cttgaaaaca
    28501 aaggttttgt tatacaaaat gcagagaatg gtaagtattg gctaggaata aatttatatg
    28561 caataggtat ggttgtggga gaaaaaatgt cattgactga aatagtaaaa ccatatacga
    28621 aaaaattaaa tcaagaattt aacgaagtag ttaatgtatc tatattagaa gaaagagctc
    28681 aagattctcc aagaagtatc ataatacata aagagtatgg ttcaaatcaa ttactttcag
    28741 taaatccatc cgttggttca agtagtgaat gttattgttc agcagttggt aaatgtctta
    28801 tggcatttaa tgattctata gattttgaaa aatatagaaa aacaccaata cacaagtata
    28861 cagaacatac aattgataat tgggatgata tgatgctttt tttagcaaaa ataaaagaac
    28921 aaggatatgc tatagatgat gaagaactag aacatggact aacttgtatt ggagcaccta
    28981 tcttagataa aaataataaa gctattgcag ccataagttt gtcaggtcca actataagaa
    29041 tgagagaagg ggactttgag tataagatta aaagagttat agaaactgca aaaagtatat
    29101 ctgaactttt tagatgattt ttaatctcga taataaaaat aggctatatt taaattgtta
    29161 tttgtaaata tcagtttaaa tgtagcctat ttttctttaa tctaaaatta aatattctta
    29221 aaatttaaaa tgttattgaa atatataagc gaatgtgtta tcttttaatt aagcaataaa
    29281 aatagaacaa ggttctatac tatagaacaa aattgatgca tatatagtat ttatacaaaa
    29341 tataaatatt atatatttaa aattaagtaa aaatattaca taaaggggag atttgagaaa
    29401 tggaaaaagt caagttgagt gttgaaagat gtaagggatg ctatctttgt attgaagctt
    29461 gcaaaaaaga tgcaataagt ctttctgact acacaaataa aaaaggcttt gtaactatta
    29521 aagtcgatga agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc cctgatttag
    29581 tatttgagat attataaatt atttttatta gaggggagaa aaggtatgca aagtttatta
    29641 attaaaggaa atgaagcttt agcagaaggt gctatacgtg gtggctgcag attttatgca
    29701 ggttatccaa taacaccaca atcagaaata ttagaaatga tggcatggag acaaaaagaa
    29761 gttggaggag tatttatcca aggtgaatca gaaatagcca gtgtaaatat ggttatggga
    29821 gcaagtgcac ttggagcaag agctatgact tcttcatctg gtccaggatt ttcattaaaa
    29881 caagaaggta ttgcatattg ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa
    29941 agatatggta taggtgatgg atttatagga gctggtcaag ataattattg gcaagcagta
    30001 aaaggtggtg gaaatggaga ttatcattta atcgtgttag cacctaatag tgttcaagaa
    30061 aatatggata tggcatatga atcttttgaa ttggctgaaa aatatatgca tccagtactt
    30121 atactttctg atgctacaat aggtcaaatg gtagaacctt gtatagcacc accaatgaaa
    30181 gaatacgata tggataaagc tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat
    30241 aaagtaataa ctgatataac atattttgaa gaccaagata catggcaaaa agggtatata
    30301 gaaaaaatga gaaaagttca agaagatgaa caaagatggg aagagataga aatagaagat
    30361 gctgaagtgg tatttgtggc atacggtatt tcatcaagaa tagcagaagg tgcagttaga
    30421 gatgctagag caaaaggaat gaaattaggt ctaataagac caaaaacatt gtggccttac
    30481 ccaagaaaag catttaagaa attaagtgat tcattgaagg gacttgtaac agttgaaatg
    30541 aacatgatgg cacagatgaa ggaagatgta attatagcat gtgataatag atttccagta
    30601 tattcagtag caacagctca atatatggca gaagtaaatc aattaataga ttttgctcaa
    30661 cagattatag atggaaaagc taagcaagag gaggtatttt aattatggct aaaaaagcac
    30721 cagcaataat gatgtgtgaa aatatgttct gtggaggctg tggacactct atagtcgata
    30781 gaattattgg agaagtatta acagaaatgg atatatgtca agatacaatt atatgttcag
    30841 atataggttg taatcatatg tttcaattta gtatgaatgt agatgttgtc gttccacctc
    30901 atggtaaaat gggagcagca atggcagcta tgaaaagagt tagaccagat aaatatatat
    30961 ttacaatagc tggagatggt ggggcttatg caatagggct tggagaaact atgtcagctg
    31021 ctactagaaa tgataatgta atatttcttg ttatgaacaa taatatgttt gcaatgacag
    31081 gagggcaaat ggctccaact actatagaag gtcaatatac agcttctact ccagcgggta
    31141 gaaattacgc agatcatggt tcaacatttg atgttgtaaa agttatggga aatttagata
    31201 tagcatatct agcaagagga acaataacta accctaaaga aataaataca acaaaaagat
    31261 acatcaaaaa agctctagaa aaacaaaaag aaaagaaagg tttttgctta atagaagtgc
    31321 ttgtaccatg tccaacaaac tggggattaa agccagtaga tgctatgaag agaattgaaa
    31381 atgaacttgt tccaaagtat ccacttggag aatttattga ttagggagga aataatatgt
    31441 tgaatgaaat tatatgtgct ggttttggag gacagggagt tcttacagca ggaaaaatta
    31501 taatgtatgt tgcatataag gcagaaaaga aaattacatg gttcccatct tatggaaatg
    31561 agatgagggg aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc
    31621 cttgtgtaaa aaatccagat atactattgg gaatgaacag cgtatcagta gataagtatg
    31681 aaagtttaat gaaaaaaaat ggttatatgt ttgtcaatga atcactagta agtaaggata
    31741 gaaagtacag agaagacatt aatgttataa aagtacctgt tactgaaata gcacaaaatt
    31801 taggcaatga aaaagctgct aacatttgca tgttaggtgc tatggtaaag agtttggatt
    31861 tatttacaaa agaagaattt gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag
    31921 gtaaattcaa ttctaaaaat gtagaagcat ttgaagctgg atacgactac gaatgaatat
    31981 tttaaatatt taaaatgatt attgaaatac ttatttttat atgttagtat ttaattaata
    32041 aaatatagaa cgtcgttctg gacaatagga cgaagttttt aggtcggaat acaagaacgg
    32101 cgttctgtat agtagaacta gggggtgtta aattgaaagt tttagtaata aatcctggtg
    32161 gtacatccac aaaaatttct gttttccaag atgaaaatga aattttgaaa aaaaatataa
    32221 cccatacaag agaagattta aaaaattttt ctaaagtatt tgatgaatat gattatagaa
    32281 aacaactgat agtagatatt ttaagtagtg agaatcatag tataaattct tttgacgcag
    32341 tagttggtcg tggtggactt atgaaggcta taaaaggtgg tacctatact gtgagtgaag
    32401 aaatgattga ggatatgaga aatgaaataa atggggaaca tgcatcaaat ttaggtgcat
    32461 tattagctaa aactatagct gatgaaatag gagtacaatc atttgtagta gacccagttt
    32521 ctgtagatga atttgatgat gtatcgagga ttacaggaat atcagacata gaaaaagcaa
    32581 gttggttaca tgcattaaac cataaagctg tttcaagaaa aattgcacaa aaactagggg
    32641 gaaaatacga ggattataac tttattgtag ctcatttagg ttcaggaata tcgattgtag
    32701 cacataaaca aggcaaaatg attgatggaa gtggtggtag gtctgatggt ccattttctc
    32761 cagaaaggag tggtggatta cttgcatatc ctcttataga attgtgttac tccggaaaat
    32821 atactcatga tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac gattatttag
    32881 gaacaaagga catgatagaa attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac
    32941 ttataatgga tgcatttatt tatcaagtag caaaagaaat ttctatgtat ggagcttcac
    33001 tttcagggaa tgtagataga gttatcttga caggagggat atctcactca gaaatggtag
    33061 tcaaaggagt agcagaaaaa gtttcatatc tagctccaat agagatagta ccaggagaga
    33121 tggaaatgga ggcacttgct ctaggggcac ttagagtact taatggagaa gaaatagcaa
    33181 aaacttatta atatttagga gaaagagtat gttaaaagat tttaataatt tattagaaga
    33241 ggctaaaaag cttgaacctg ttattgtaag tgttgctgtt gcagaagaca aagaagtatt
    33301 aaaggctgtg aaatcagcaa cagatttaaa cattataaag ccaattcttg taggagacaa
    33361 ggataaaata gaaagtatat caagagaaat tggattaaaa ggtcatgaaa taataggttg
    33421 tcaaaatccc gatgaatcag ttaaaaaagc tgtagagata gttaaaaata aggaagctaa
    33481 agttcttatg aagggattag taaatacaag tgtctatatg agagctatat taaataaaga
    33541 aacgggtctt agaacaggaa gattattaag tttgcttgca gtatatgaat tacctcaata
    33601 tcataaactt ctttattgta ctgatagtgg aataaatgta tcgccaaact taaatcaaaa
    33661 aaaagatata atgacaaatg cacttcttac tatgaaaagt ataggcatgg aaaatcctaa
    33721 aactgcgata cttactgcaa atgaaatggt agaccctaag atacaatcta cagtggatgc
    33781 caacctactt gtagagatgg aaaaatcggg agaaatacca aagtgtatag tggaaggtcc
    33841 aatagcattt gatgtagcat ttgatgcaca tgcagcagaa cataaaggta ttaaaagtaa
    33901 tattgctggt gatgttgatt tattagtatt tccaaatata gaagcaggga atatattagg
    33961 aaagtcatgg cttagattta ataaagctaa gtgggcaggt atagttttag gagcaacaaa
    34021 tccgataatt ttaggttcac gttctgacac agctgaaata aaaataaatt caatagcatt
    34081 agcgtgccta gcatcacaat aagaaattat tattaaggag gaaaaagtat tatgaaatat
    34141 gcaaaaagaa tggatatggt aaaagcttca gctataagag attctcaaaa gaaaatagct
    34201 cagaaggtag caagtggtgg aactgttata tcttttgctg cagggcttcc tgacccaaat
    34261 ttatttccaa tagaagagat aagtgaaata actcaaaaag tacttaatga aaggggaaaa
    34321 tttgccctag catatggacc tactaaaggt gaagatgagt tgttagatct attagtaaaa
    34381 agaatgaaag aagaagaaaa tattgataca aaagcagaaa atattataat aactactggg
    34441 tcacaacaag ggatagcatt aagtgctatg attttattag aaaagggaga tgtagtagtt
    34501 acagaaaatc caagttactt aggtgcaata aatgcattta gaccttatga atgtgatttt
    34561 ttaggagtag atacagatga agaaggaatg gtaacagatg atttagacaa attattatct
    34621 gaaaattcta atataaaggt gatatatgta ataccaacat ttcaaaaccc tacaggaaaa
    34681 acatggtctt tacaaagaag aaaagatttt atggaagtcg tgaataaata tgatgtcata
    34741 gtaatagagg ataatcctta tggagaaata agatttacac cagaacctct acctacactt
    34801 aaatctcttg atactaaaga taaagtattg taccttgggt cattttcaaa ggtattatgt
    34861 ccaggattta gagttgcttg gatgtgtggt aatgtagaca tcatagataa ggcagaactt
    34921 ttaaaacaag gtatagattt acagtcaaat caattttcgc aagtacaagt aatagaattt
    34981 ttaaagaaat acaacttaaa tgaacatata gaaaaaataa gagcagagta taagaaaaga
    35041 tgtctgctaa tgttagatgc tatgaagaaa catttcccag aggaagctaa atatacagaa
    35101 ccagatggag gaatgtttat atgggttgaa cttcctgaaa gtataaatgt agatgattta
    35161 ttagataaag ccattgatgc tggagttgct tatgtttctg gagagtcctt ctttgcaaat
    35221 aatggtccaa aaaatactat gagacttaac tatacaacaa tgtcagaaga acaaatcgtt
    35281 aaaggtgttg agatacttgc agaagttata aaaaaagaac tcaattatgc ttgtgaataa
    35341 atataaaaag tgagggttta aatatgggag aaaaagaaaa cggtataaag tttggaaagt
    35401 taatgtttca acttgttatt atagcaatat cttgggaatt agtttatatt ataccattta
    35461 tccaatatac attatatgat ccaatcctaa aagcattaga atgtacaaac actcaactag
    35521 gttttctact tacaatttat gggttaggta atatatttgg agctcctata ggaggctggt
    35581 tagcagatag atttgattat agaaagataa ttttaggttc tgtatttctt aatggagtag
    35641 tatcattttt atttgcattt aatatgaact atccatttgc agttgctact tgggtaggtt
    35701 gtgcaatcac atcattagtt atgaactatc cttctatggt aaaaatactt agagttatag
    35761 gaaaagataa tcaaggtaag gtttatggat ttaatgaagc tatggttgga gtttctggag
    35821 tagtaatggg agcaatattc ttatatattt atacatgctt tgcaactcct acattgggta
    35881 tgagatgggt aatgatatct ttaggaatat tatcaatagc aatgtgtcca gtattatggt
    35941 ttgtaattaa agatgtagat attaaagaag aaagagaaga agcaggtgaa aaaatgtctg
    36001 ctggtgattt tataacagta ctaaaatcac caaatacttg gctagtagga ataagtatat
    36061 tctgtgtata ttcattcaca gttacaatgt cttattttac accatatata acatctgtac
    36121 ttggaggttc tgtagcatta tctggtgcat tagcaataat aagacagcat ggtctaaaat
    36181 tatttggagc accatttgga ggatattgtg cagacaaatt aaaatctcct acaaaagtac
    36241 ttttaccaat atatgtattt ggtatagcag taatagtttt attcctagta ttacctgcat
    36301 caactccaat gactatattc atagctttaa catttgtagt aggtatatta ggttacatgg
    36361 gaaaaggtat atattatgca gtacaagatg aagtaaaagt tccagttaaa tattcagcaa
    36421 ctactatagg tatagctgca gctttaggat tttcaccaga tgtattccaa tttgcattaa
    36481 taggacactg gatagatact tatggcaata aaggttacac atatacattt atattccaaa
    36541 tagcaattct agttattgga atactatctt gtttatatat attaaaggtt aaaaagagaa
    36601 gagcaagtaa attagaagct taaatattca atttggtaat taagtatata taaatacaat
    36661 acttattttg atgaatgtga ttgtataaat aacataaaaa atatcaatag attaagtttt
    36721 ataataatgt ttagagaata aagtataaaa atcaaaatgt tatcaagttt tctaggtttt
    36781 aacataaagt tacccctgaa ggtgtaaaat tgagtggcaa ttattatcta ttttcttacc
    36841 tacataaacc tatttaattt aaattctaag agattttcaa aatagatggt agttgtactc
    36901 aattttaaac ctcataataa atacgaaaat ttaaaatcta actagatact atttaattat
    36961 atattaataa atatatattg tactatttat ataataataa tttcaggctt ttcattatat
    37021 gtattccaat ttatattgtt tgattattat tcaaacacac gtcgttaaaa attttataaa
    37081 tttatgttta gatatgatta atactcttga ttttagaaat aaatatttgt agattgtaga
    37141 ttaaagataa tataaataaa tagaaggtag attttttaga tatattaata taaagattat
    37201 ataaaatatt gaaatatagc tgtttaaatt attgtctata aatataaaat gaatggtggt
    37261 gagttaagtg accgatttag ttaacaccat taatggaatt gtttggtcac ccgtattagt
    37321 tgcaatgtgt ttgtgtatag gattatattt ttcattgaaa ttaaaatttt ttcaaataag
    37381 agatgtaaaa gaaatgttta ctctcttgct tgaaggagat agttctcatg ttggtatatc
    37441 atcatttcaa ggttttgcta catctatggc tggaagaata gggactggaa atatagctgg
    37501 tgtagctgta gcaatagcaa tgggaggtcc aggagcatta gtatggatgt gtataatggc
    37561 tacattaggt tcagcaactg catttgtaga atctacttta gctcaaatat ataaagatga
    37621 gcattatggg cagtatagag gaggacctcc atattatttt gaaaaaggtc ttgggtggaa
    37681 aaagtgttca gttttatttt caatagttgc aataataagt tatacatttt ttatgcctgg
    37741 tacacaatca aacactttat atctagcaat gaatcaagca tttggatttt caaaaatgac
    37801 aatagcagta gcatcttcta ttgcattagc gataataatt tttggaggaa ttaaaaggat
    37861 tggtttattt gctgaaaaag tagtacctat aatgggtgga gcatacctta ttatgacatt
    37921 agtaataatt ataacaaata ttagtttagt tccagaggct attgtaacaa tgtttaaatc
    37981 tgcatttgga tttggttcag tttttggagg aatgctaggt tcagcaatat cttggggagt
    38041 aaaaagaggt atatactcaa atgaagcagg agaaggttct gggacttatg gtgcggcagc
    38101 agcagaagta agtcatccag ccaaacaagg attagttcaa gcattttctg tttatgtaga
    38161 cacattatta atatgtatgg ctacaggtat tatgattgta ataacaggaa tgtataatgt
    38221 tacaggtgtt tgcacagcac taccaggtgt agaaccggga ccacaatatg tacagagtgc
    38281 aataaataca ttacatccac aattaggtga tatttttata gcaatagttt tattattatt
    38341 tacatttaca actttattag ccttctcata tatgatggag actaatgtat cttacttagt
    38401 aactagaatt aaagcagaaa aacatgaaaa gtacataatt aatgtctgta gatttatgct
    38461 tatagcatgt gcagcaatgt gttgttttat agaaccactt acaagctggg cattaggtga
    38521 tatatgtata ggtgtattaa catatataaa tttaattgcc atctttacac taaaaaaacc
    38581 agctataaag gcatattatg attatataag acagaagaag atgggaataa aaaggagtga
    38641 tagaactttt aatcctatag agttagatat aagaaatgct gagttttggg aaaatagata
    38701 cttaaaagag gataagcatg agttaaatta aagttataat aaaatataaa aattactaaa
    38761 taaatttagt gggaggtaag attggtatga aagaaagaat aatacaagtg gctgattcaa
    38821 agaaagaaaa aattctaggg ctatgtcaat ctttatatga tgaaccagaa attgctttgc
    38881 aagaatataa atctgctaaa aaaatatcag aatttctaag agaagagggt tttgatgttg
    38941 aggaaaactt ggcaggtatg gctacagctt ttaaagcaac caagaaaaat ggagatggtc
    39001 ctaaaatagc atttatagca gaatatgatg ctcttccagg caatggacat gcatgtggac
    39061 atcatttaat tgcatctatg ggagttggag ctggaatagc tctttcaagt atactggata
    39121 cttacaaggg agaagtaagt ataattggaa cacctgctga agaaactgga gatggaaagc
    39181 cttacttgat agaacatggt gtatttgatg gctatgatgc agcaatgatg atacatccaa
    39241 attcaaaaac ttgtgtaaca cctgaaatta tagcaatagg aggtctagat ttcatattta
    39301 ctggaaaagc atctcatgca ggagctaagc catataacgg aataaatgct ttagatgcag
    39361 tagtactact atataataat ataaatgcac taagacaaca attagtagat ggaacgagaa
    39421 tacatggtat tatactagaa gcaggaactg ctgcaaatgt aatacctgat atgggaaaag
    39481 taagattaga gataagggca aaagaacaaa attactttga tgaagtagta gaaaaagtga
    39541 aaaattgtgc tagaggtgct gcaatagcca ctggatgtga acttgaattt tatcactttg
    39601 aaccaacttg ccaaggatta aatgaaaata aagttttggt agacatattt acaaagatta
    39661 tggaggaatt tggaatatat gaagatgaac aagtaatgtt aggttcaaca gatatgggaa
    39721 atttaagtca aataatgcca tgtatacatc ctttgatgaa gttttctgaa aatggagaag
    39781 aactacatac aaaagaattt ttggaggctt ctataaattc atatgcaaaa gatagagtaa
    39841 ttgatggtat aaaaatatta gctttaacag gatttaattt atttgaaaat ccagaattat
    39901 tgaaaaaaat gaaggaagag tagaacttag actattttta attatactct ttaaaataag
    39961 aagagtgtct caaaatgaac tttttagttt atgagacact cttttttgta tgaaattaaa
    40021 tatattttta ttattttaaa catgaaaaag aggcttatct gtttttatat aacctttttt
    40081 aacttgtagc acaaaatatc atgaatgatt acaattcgaa tgataaaatc gattataaga
    40141 tatataggag gggtacatat ggaaacttta gaagtaagaa atattgaatt gagtggtaca
    40201 aattatgaga taggatacag actaggagaa ttggttgcta atatgccaga gataatagaa
    40261 ggtcagatta ataaatctaa tctggtaagt aaaaaagaag aaaaagagat gatagagttg
    40321 tttgataaat attgtccagg tcttaatgaa gaattgcaag gttttgctga tgcaattcaa
    40381 gtgaattgta atcaaatttt atattatact atgacttatc ttaaaccagg ttgtagtcaa
    40441 gttgcattag caccagaatt aactgaaaat gggcatgtac tatttgcacg taatttcgat
    40501 ttctcacaca atatggagga ttttgtttta tgtaaaacta aggtaaatgg aaaatatgca
    40561 catataggta ctaccgttat gcaatttgga agaggtgaag gtatgaatga atgtggctta
    40621 ggtgtaagtc agtcatcatg tggtattcct gtaggcaatt cagatggttt aagaaaacca
    40681 gcaattgtag gtttacagtt ttgggctgtt attagatatt tgttagaaaa ttgcaaagat
    40741 gtagatgaag cattagaata tttaaaagat atgccaattg catacaatat taatttattg
    40801 cttgcagata agtctggaaa cattgcactt gtggagactt tagatgggaa aaaagaagta
    40861 aatataatca atagtttaga aagtaaaaga gaatattttt tacactctac taatcataca
    40921 catattgata aacttcataa attagagcca caatcaatga aaaattctat tcatagatat
    40981 aaattaatta aagaatatat aaataagtct aaaaaaattg gagagaaaga gttaatgagt
    41041 ttattgtctt caaaatatcc aaatggctta tcttgtaatt actataatga tttttttgga
    41101 acattaaaaa gtatagttat ggatttaaat ataggaaagt ttaatatttt atggggtgga
    41161 ctagagaata aatgggagag ctactattta aaaaatgaca ttaaatctat aacacagagg
    41221 ataaacataa atattgaaaa agcaccatct gacttttttg atttcattga ataaaaaatg
    41281 tattcaatta ttctaaataa ttaggatata taaatcaact aaaagttttt atttatcgta
    41341 ttgaattagt tataatattt tagtatagta aaaaatagtg ctattttaca tatttaaaga
    41401 gtttaagtag aagagaggtg ccatattgga ttacttaaag tattttgaaa aagctgtaat
    41461 gtatattgag aataatttac atgaaaatat aactgtaaat gatgtggcaa aagaaactgg
    41521 atattcttat tatcatttaa cacgcttatt taagtctatg tttggtgaga gtgtaggaag
    41581 ttatataaaa aaaagacgat tagtaagtag tacaaaagaa cttttatata gtgataaaaa
    41641 agtgatagat atagctattg gtagtggatt tgaatcttcg gaagcattta gcagagcatt
    41701 taaatctata tacaaggtta gccctataga atatagaaaa aatagaattg atgtatttgt
    41761 tggtaaaaag aaaaaattag agttagattt tatgagacat cttgttggaa atataacgat
    41821 taaaccagtt ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg ataaagtaat
    41881 tttagaaaat aattcattac ctgacttgtg ggagaaattc agaaaagtac atcatattgt
    41941 cccaaatact ttgccatcaa agagagtttt tggaatctgt gaagcaatct ctgaaataca
    42001 cctagttagt gaaagtatgg aatttaatga gataatagga cttgaagtta atagttatga
    42061 tgtgattcca aattcatttg tttcaaaaac tattaaggaa ggaaagtatg ctatatttac
    42121 tcatactggt agcttagata atttagacaa aacttatgaa tatatatggg gtacttggtt
    42181 tttaagttca aaagaagagc ttgatataag agatgatttt gaagtatatg atgaacgatt
    42241 tttaggtcct agtaatgtga actcacaaat tgatatttat atacctataa aataatctat
    42301 aagaaaacta aaaaataatt tgattaagaa atagatgttc gtaaaagagc atctattttt
    42361 tatttttatc agatttagat aattttaata ataaaagaaa taaaaataag ttcttaaatt
    42421 tctaagaaga atttagaata tagtcgaaat aagaattgta tatttctgaa aattctaatt
    42481 agtacaattt tttcctttat aaaattgaaa aaagatgtat ttatttatca aaaatattat
    42541 attaaaatga tattaataaa aaaacattta caatattagc atatagtaca gaaaaaagat
    42601 gtaatttgta aaatttatgg taagcagtaa aattaaaaat ttaaagatat tatagggagg
    42661 ttataatttt atggaaaacg ttattacagg tctaagtaaa ggtgcagagt ggtttatcgg
    42721 tttgtttcag aaagggggag aacaatttat aggtcttgtt tcaggtactc ttccaacgct
    42781 aattgtatta atggtagcta ttaactcttt gattaagata attggtgagg aaagagttaa
    42841 taattgggct tctaagctag gaaagaactc ttttacaaga tatattctac ttccattagt
    42901 atcagtattt ttcttgggaa atcctatgtg ctatacattt ggtagatttt taaaagaaga
    42961 gcacaaagca ggattttatg atgcagcagt ttcatttgta catccaatta caggtctttt
    43021 tccacatgca aatgcaggag aactttttgt atggttggga atatctgcag gtttgactac
    43081 tttaggtaaa gaaactacta cattggcatt gtggtatttt atagttggtc taattgtaat
    43141 ttttataaga ggtatagtaa cagaaaaaat gtatgctttt ctaacaagaa aaaatagagc
    43201 aacaaataag gcttaggagg tagataaaat ggaggagaat aaaatattaa aaatagaaaa
    43261 gggaacttca ggctggggag gtcctttgta tataaaaaaa gaagggaata gaaataagat
    43321 actgtcaatg actgctggtg gtatccatga agtaacatta aaaataaaag aactactggg
    43381 ttgtgaaatt gtagatggat ttaaaactgg agtttctgat gaagaagtag cagtagttat
    43441 tattgattgt ggtggaactg ctagatgtgg agtttatcca aagaaaaaaa tacctacaat
    43501 aaatgtaaat ccagttggaa aaacaggtcc tttagcaaaa tttataacag aagaatatta
    43561 tgtatcagat gttaatccta actgtataag tgttgtagat ggggaagata tgccacaaaa
    43621 atctcaagaa aataaaagcg aaaataagag ttctattaga aagcctgata attatgatga
    43681 ggttaagtca aaagcacaag gtgaatatgc taaaaagaac ataatacttt ctattggtca
    43741 aggggcaggg caagtagtat caaaatttta tgatgcaggt cgagatacta tccaaatggt
    43801 aatgaataac gtaataccat ttatggcatt tgtaagtatg cttatgggaa ttattttagc
    43861 atctggactg ggagactgga ttgcaagagt tatatctcca cttgcaggta atattggtgg
    43921 attattgatt atatcagtaa tttgcacact tccattctta tcaccaatac taggacctgg
    43981 tgcagttata gctcaagtag tagggactct tgtaggtaca caaattggtc ttggtgcaat
    44041 accagcatat ttagcacttc cagctttatt tgcaataaat gggcaagctg gatgtgattt
    44101 tgtaccagta ggattaagtt tgggagaagc agaaccagaa actgtagagt atggtgttcc
    44161 agctttattt tactctcgtt taataactgg tccaatagct gttattattg catatggagt
    44221 tgcagtattt gcattgagat aatatttaat gtcaatataa ggcttggaat taattaataa
    44281 aattgtaaat ataaatttaa gctagtcaag attttttgtt aatgaaagac tagcttttat
    44341 actattaatt agctgtaatt atcgagttag attaatacta aattttcctt tattaaagtg
    44401 aaattttatg tattaatttg gtttcaaatt tgtactagaa tgaaatcaag gaggagatac
    44461 aaaatgagtg taactgagaa aattccttct attgaccgca agatatttaa gatattgatt
    44521 atgtgtagta aaaaagagtt tgtgagtatt aactcaatag caaatgaatt gaatgtcaca
    44581 actagaagtg taaggacgta tataaaacaa ttgaataaag atttaggtaa tgatatagca
    44641 gtaatcaaat atataaaggg tcaaggctat aaactagaaa taaaagatga gcagatactc
    44701 aataagatta ttgatatgaa tagaaaaaat gtattttcat taaattcaaa agaagataga
    44761 gtagagttta tactaaatta cttgatagaa ctagatggat ttattacact tgatagttta
    44821 gctgatgaga tgtgtgtagg aagaactact ttagtaaatg atttccagta tgtagaaaaa
    44881 gttttggctt catataattt aaacttgatt aaaaaacaga atacaggtat gaaattaaat
    44941 ggaaatgaac ttgatattag actgtttata ttaaatcagt tgtataaaaa ctcaaggaaa
    45001 gatttcagta attcaaagta ttttaaagga atcaaaaaag aagaaatcat aaatttagaa
    45061 gaaaaactat tgactttatt taaaaaaaat aatttttatg ttacagatga aatgttaaga
    45121 gaagttatta actacatagt agttttagtc tatagagtaa aagaagaaaa gaaagtaaaa
    45181 gattatgatg taaagtttga tttacttaaa tcatatgatg aatactttat tgctagggaa
    45241 ataaaacata taatatcagg aatgtttgaa tgtactttaa atgatgaaga aattatttat
    45301 ttaactatac cgttagttag tggaaatgca ccagcatcag aatgtgcttt aaatagtagt
    45361 agaattagta aaaacattga tgagttgatg gaaaatatat ttaatcaaat atatgtcgat
    45421 atgggtattt ttataaatga ggatgaatta agagtaggtc taggatatca tcttagtttt
    45481 actttaaaca ggctattatt taatataaag ttaaaaaatg ttttgttaga agagattaag
    45541 caaaattata tacttccatt taaattagca caaatagcag gaaaagtaat agagaaaaag
    45601 tataacttag aagtttcaga agatgagatt ggatatatag caatccattt cagtggatat
    45661 ttagaaagaa atagcagtag attctattca ataaaaaaaa tagcaattat atgtagtaca
    45721 ggtctaggaa cagcaaaact attgaaaata agagtagaaa aactgatagg aaataatcct
    45781 aaaattgata ctctttcaag ttttaatttg agaaatatta acttagatga atacgatata
    45841 gtttttacaa ctattgattt agacacatca aatataaata caatagtatt aaaaataaat
    45901 acaatatttg atgaaaacaa actaagagaa caattaaaaa cagtattgtg cctaaaagaa
    45961 gggaatattg acactactaa ttctacaaat ttactaataa ataatttgtt agatgaagat
    46021 aaatttatga tattaaatga aaagactata cttgcctcat tagaaaaaat gatggaccat
    46081 cttatggatt tgggatgtat tgatgagaag tttagaaaaa atatagtcaa tagagaagaa
    46141 aaatctccca cagtatttga taaaggttta ttatttcctc attctataaa tgaaaaatca
    46201 gataagtttt taatggcagt tggaatttta gaagaaccaa tagaatatgc aaatagaagt
    46261 ataaaaataa ttcttatgac aatgtttcca tgtgagaata agatagactc agacttatta
    46321 gtaaaaattt atgaagaagt tttaaagata ggccaagatg taaaattaac gaataagata
    46381 agtaaatgta gaactttttt agaatttaaa aaggtgttac ttaaaaacct tatataacta
    46441 ttgtacagca tttatagttg ttatacagat ttaaaaataa tgtgattttt ggaaggtgta
    46501 taaatatgaa attattagta attttatttg ttgcttatat tttaaatact atatttataa
    46561 tgaagcagaa taaattgtat tttaaaactt taaatagagc taaaaaaaag ggtgatatag
    46621 tgtctacagg caaaaagaaa agctattttt ctaaaggaag tatagccgtt atatcttcag
    46681 attcagatgg gtttataaag tttggagaaa ttttaaaagg gcgtacagta atggctaaat
    46741 ttaaagagat tgaagaaata gaaggattag atatatatag tgcagagcaa aaatttaaag
    46801 atgaagagag tattgtacaa gctataaaat ttataaaaga aaaaataaat gtttctttta
    46861 attaactcaa tataaatata aattatcagg aggaatgtca tgaaatatga agttaagata
    46921 agtgggatag gcagtttagt taaagagtta atggatgaaa gcaattgtct tattatatat
    46981 gatgaaacca ttaatgatga cgacttaaag gatatatctg taattcattc aatagcaacg
    47041 ttaaaaagtg atgttgaaat aggagataca cttactatag ggaatagaga ttattgtata
    47101 gtttcagttg gtgatattgc tcagaagact cttagagaaa ttgggcattg tacaatcaaa
    47161 tttgatggaa aatgtgaagt aaatttacca ggagaaatac atgtggaaat aggaaatcca
    47221 gatataacta ttggagattt aataactatt tcttaatttg aaaataaaat tattataaca
    47281 ggaggaattg gtatgttaga tttaaacaga aaacttgcaa aattggaaga ggaaggtaaa
    47341 aaaataaaag tagctcttat aggagctggg catatgggaa atggaatggt aagccaaatg
    47401 gcaaatatga aaggtattga agcttctatt gttgtagata tagatttgga gttggctcat
    47461 aaagctttta ctgatgcagg aatagatgaa gaaattatag ataatgtaac aaatgaaaag
    47521 gatgcagaaa taaaattaca agaaggaaaa gtattgacat gtaatgactt tcttgtggct
    47581 tgtaaaacta agtcaataga tgtagttata gatgcaactg ggggtattgc aattggtgca
    47641 gagatagcat taaactctat tttaaataaa aaacatatag ttatgttaaa tgctgaaact
    47701 gattgtgttg ttggaccaat acttaaaaaa ttagcagatg atgctggagt aatatttaca
    47761 ggttctgcag gagatgagcc aggtgcagta atggaattat ttgattttgc agatgcaatg
    47821 gggtttgaag ttagagtgat aggtaaaggt aaaaataata aattagattt agattgtaat
    47881 cctgatacag ttagagaaga agcagaaaga aaaggagctt cacctcatat gatagcttct
    47941 tttaaagaag gaactaaaac aatggtagaa atggctttaa tgtgtaatgc aactggattt
    48001 gtgcctgatg taagaggtgg tcatggtatt gaagctacag ttaatgaagt acctaagaaa
    48061 tatgcattaa aaagcgaagg tggagtatta gataattatg gtgtagttga ttttgtaaat
    48121 ggtatagcac ctggagtttt cgtagtagtt gcccataaat taaaagcagt taatgatgaa
    48181 ctaaaatatt taagtatggg tgatggacct aattacatat tatatagacc atatcattta
    48241 tgtagtttag agactccttt atcagcagct atggcagttt tagaccataa ggctacaata
    48301 gtacctaaag ctggactagt agcagaagtt atgactatag ctaagaaaga tttgaaaaaa
    48361 ggtgagttta tggatggcta tggagcatat acatgctatg gtactataga aaaatacgat
    48421 gtagcaaagg ctatgaatgc tgtacctatt ggtttgataa gtaaaaagac taaagttgta
    48481 aaagatatta aaaaaggtga agttataact tacgatatgg tagagataga aaaagatact
    48541 actttatatc atttaagaca attgcaagaa aaaatatttg ggtaagattt gatggtaata
    48601 taaatagata ttagtaaaaa gggttgatgg ttatttttaa atcaaccctt ttaaattttt
    48661 atatattctt gttaggatac tttctttaaa agtatattat aggttatttg tagtcgtaaa
    48721 aattattttg aattagttct gcaattttat attcacattg aaataatatt attaaaaatt
    48781 ttaatataaa atgaattaaa atgaccaatt atgtattata atattaactg taatactaat
    48841 atagttctat taattaaaga aatatccccc attaatatat aataaatatc tttaagcgag
    48901 agaacttagt aaaaaatata tcgtattcaa taaaaaatct gtttacagaa tggtatatat
    48961 agttatataa tatactatat aagatgcgaa aagataaaaa gtatagcaca atgaaagaag
    49021 gtgattatta ttcagcttaa tgaaagacaa ctaaaaatta tagatatagt taaggaaaat
    49081 gaaccaataa cgagtgaaag tattgcctcc agcttgaatg tgactcgtgc tacacttaga
    49141 tctgatttag ctatattaac aatgacagga atattagacg caagaccaaa agttggatat
    49201 ttttattcag gagttagtga aataaattta attggtaaaa gtgtaaaaga aaaaacagta
    49261 gaggatataa tgagcatgcc tgtactagct aagaaggatg aaagtatata tgatgtaata
    49321 gtcactatgt ttttatctga tgtagggagt atagttatta tagatgagaa tgaagagtta
    49381 tgtggagttg tttccagaaa agacttgcta aaagcaacta taggaggttc tgatataaat
    49441 aaaatgccaa taggtatgat aatgacaaga actccaaatg tagtcacttt aactaaagga
    49501 gcaagtgttt tattagcttc aagaaaaata atagaacatg aagtagattc gattccgatt
    49561 gttgaatata agggagaaga caaaaatcat atgagagttg ttgggagaat atcaaaaacc
    49621 aacataacta agctattctt agaaatagtt gacaattaag gaggtatgct ggtgaaaaac
    49681 ctaattatat atgcagtatc agattcagta ggggagactg cacaacaagt tgcaaaggca
    49741 tgtatgtctc agttttatgt gaatgaaact tatgaaataa aaagatttcc atacatgatt
    49801 aataaaggag tactacttga aactttggag aatgcaaaag ctgaaaacgc tttaattgta
    49861 tatacattag ttgatgaaga attatgcagt attgttgaaa gatattgcga aagagaaggt
    49921 cttagttgta tagatttaat gacagatata ctaagagaaa taagcaagag aacaggaaga
    49981 aaacctaaaa gagaagctgg tataattaga aaattagatg aatcttactt taagagagtt
    50041 gaagctatag aatttgcagt gaaatatgat gatgggaaag accctagagg tgttcttcaa
    50101 gcagacataa ttttagtggg aatatcaaga acatctaaaa ctcctttgag tatgtattta
    50161 gctaataaaa atataaaagt agctaatgtt ccattagttc cagaaatacc aattcctaaa
    50221 gaagtgtttg agatagatac taaaaagata ataggtctta ctaattcacc tgaaaagctg
    50281 aatgagataa gaacccaaag attaaaagct ttaggattgt ccagtaaagc aaactacgct
    50341 aatttagaaa gaatacttca ggagttagat tattcagaag agataatgaa aagaattgga
    50401 tgcccagtaa taaacgtttc aaataaggca atagaagaaa cagcaggtat aattttagac
    50461 ataatgaaag aaaatggtct aaagatatac aaagaaatag aaatttaata atgtctatta
    50521 ataaaaatga tataaattaa aataaaaaga ttaatggggg atttaacaat ggaaactaag
    50581 tatgtttata gttttggtga aggaagcaaa gacatgaaat ctttactagg aggtaaaggt
    50641 gccaatttag cagaaatgac taagataggc ttacctgtcc ctcctggatt cactataaca
    50701 acagaagcgt gtaatgatta ttatgttaac aatgaaagta taagagcaga gataataaaa
    50761 gaaatagaag agcatttagc tactttagaa aaagatttaa ataaaacatt aggatgcaat
    50821 aagaatccat tattagtatc agttcgttct ggtgcagtat tctctatgcc aggaatgatg
    50881 gatacaattc ttaacctagg tcttaatgac aatagtgttg ttggcttagc agaggctaca
    50941 caaaatgaaa gatttgcata tgatagttac agaagattca tacaaatgtt ttctgatgtt
    51001 gctatggaag ttccaaaata taaatttgaa aacgtattag acagagtaaa agaagcaaag
    51061 ggatacacag ttgatacaga acttacaact gatgatttaa aagaaatagt aaaagaattt
    51121 aaagcaattt ataaaaaaga aataaaaaat gatttcccac aagacccaaa agagcaatta
    51181 atgcttgcaa tagaggctgt atttagatca tggaataatc ctcgtgcaat cgtataccgt
    51241 aaattgaatg atatagctca taacttagga acagcagtaa atattcaatc aatggtattt
    51301 ggtaatatgg gagaaactag tggtacaggt gttgcattta ctagaaatcc agctacagga
    51361 gaaaataaat tatttggtga atttttaatg aatgcacaag gtgaagacgt tgttgcaggt
    51421 ataagaactc ctcaaaatat atcaactctt gcagatgtaa tgccagctgt attcgatgaa
    51481 tttgtaaaaa ttactcatat acttgaaggt cattataaag atatgcaaga tatagagttt
    51541 actatagaaa atgaaagatt atatatacta caaactagaa atggtaagag aacagcagca
    51601 gcagcaataa atgtagcggt tgatttagtt gaagctggaa taattgatga aaaagaagct
    51661 atcatgagaa ttgagcctaa tcaattagac caattattac atccaaaatt tgaagataag
    51721 gcattaaaag aagccaaggt tatagcaaaa ggtttaccag catcaccagg tgcagcaagt
    51781 ggtaaagttt atttcaatgc tgatgatgta gtaaaagcaa atgaaaaagg agaaaaagta
    51841 gttttagtta gactagagac atctccagaa gatatagaag gaatggttaa agcagaagga
    51901 atccttacag ctagaggtgg aatgacatca catgcagcag ttgttgctag aggtatgggt
    51961 aaatgttgtg ttgctggatg tggagaaata aaagttgatg agtttaataa agaaataaga
    52021 gcacttgatg atgtagttat aaaagaaggt gaatacattt ctatagatgg ttctacagga
    52081 aatgtgtacc taggagatgt taagaaaaca gaagtatctt taactggaaa ttttgaaaaa
    52141 ttaatgaact gggtagacaa acacaaatgt atgatggtta gaactaatgc agataatcct
    52201 agagatgctc gtgcagctat agagtttggt gcagaaggta taggtctatg tagaactgag
    52261 catatgttct ttgatgaagc tagattgcca gcagttagag aaatgatatt atctaataca
    52321 gtagaacaaa gagaaaaagc tcttgaaaaa atattaccaa tgcaaagaga ggattttgta
    52381 gagttattta aagttatgga tggaaaacca gttaatataa gattactaga tccaccacta
    52441 catgagtttt tgcctcatga tgatgagact atagaagaac tttcaaaatc tatgggaata
    52501 aaagtaagtg atattaagaa gagaatagtt gatttagatg agtttaaccc aatgcttgga
    52561 cacagaggtt gtagacttgc aataacttat cctgaaatat gtgtaatgca atctaaagct
    52621 ataatacaag gtgctataga agcaatcaaa gcaggtgtta aagtttctcc agagataatg
    52681 gttccattag ttggagaagt aaatgagtta aaaataataa gaaaaatgat agtagaaact
    52741 gttgacgcta taataaaaga agaaggtata gaagttcctt atactgtagg tactatgata
    52801 gaaataccta gagcttgttt aactgctgat gaaatagcac aagaagcaga cttcttcagt
    52861 tttggtacta atgacttaac tcaaatggca tttggatatt caagagatga tgctggtaaa
    52921 ttcttaggtc aatatgtaga tgaagaaata ttagaaaaag acccattcca agtactagac
    52981 caaaatggtg ttggtaagtt agttaagatg ggagctaaat taggtagaga agttaaacca
    53041 gaattaaaac taggaatatg tggagagcat ggaggagaac catcttcagt agagttctgt
    53101 tatagtgtag gacttaacta tgtatcttgt tcaccattta gagtgccaat tgctagactt
    53161 gcagcagctc aagcatctat aaaaaatcct agataataat agactgcaaa atatgtaact
    53221 aaataaaaat aaaatcccct attgttcaat gtatcaaatt aagtatttgt aaattgaata
    53281 taggggattt ttattataaa aatttaactt atatagttgt aagtgaaacg tatttaatta
    53341 tattgcatat attattgtag agagaaattt caactatcaa ttaaattgag tttactggag
    53401 gggataaagt tgacaaagtg tcctaaatgt ggaaaggaag tatcatcacg tccaggtagt
    53461 atatgcccac gatgtggatt taaagtagca gatgagagtc taaaaataag atgtccagaa
    53521 cctagttgta gggctttggt atctaggaaa ctggattatt gtcctaaatg tggatgcaaa
    53581 cttagaggat ataatatttc tgaatttgtt aacatggtga gatgtaaaat agatgagaca
    53641 tttgataaaa aataattaaa tttagagtta agcttttgag ggtgtctcaa aatgaacttt
    53701 ttagttcata aggcactctt tttgtatgga atcaaatata tttttataat ttaaacaata
    53761 aaaaagaggc ttatctctat tttgagatag cctctataaa agtatacagt tttataagta
    53821 aatatatttt tactaatcta tttctattat ttgccttctg aatcttccat gactaaagcc
    53881 acgagtgtgc ataactattt tttaatcaaa cgtatgttaa agtaatcttt ctatttacca
    53941 gaattatttt ccagtattaa gtatccaagg tttttctata ccatttttta gtgagttata
    54001 attcacaata agagtgtcat agctattttt agcatcttca ttacttacca cttgtttttt
    54061 ataatcagtt agagtaatta atcctaaatc atatcttaat ttagtatttt ttaattgttt
    54121 ttctgataaa tcaagttgtt ttttagtaat ctgaattgta tcttctaaag caagtaattg
    54181 agcatagcta tcaattaacc cttttttaag agctttttta ctgtcttcta atgttactag
    54241 accattttcg gtattaaatt tttgttgaag ataatttgca taagtagtaa ggccagcagc
    54301 ataagtctgt ctttcaagat tgtatgcttc aacttctttt tcatatctct tgtagtcttt
    54361 ttcatactta tccgtaagaa ctttatattc atcatcagtt aggttacctt gctctggttt
    54421 tgtaggagca actggtcttg taagattagg tctatctggt aaatctgctt tgttctcatc
    54481 tttatcatta aagctttctt ctgtacgttc taaaatcaat tggtcgtatt ttaaatattc
    54541 attaacctta ccttccatat atgaatcaac tgagccacct actctaaaag attcaaatct
    54601 atattcttta tccaattgat aattatttaa atctatatta gtcaacaact taaagtagtc
    54661 ttgattgttt tttagttctt gaagtttagc tttttgagta ttttgtagtg tttgaagttc
    54721 aagttcagct gattcagttt ctaaagatgt tactaaccct aaatcttttt ttaatttcat
    54781 atctttaatt tctttagttt ttatttcaag attgtttttt atcttgtcta gctcattttc
    54841 tcttgaaact aaatcgttaa atttattagt aatatcattt gctatttggt cctccataaa
    54901 gcctttagcc tgttcacttt gatttttaag aagtccaatt tggtcgtatg gaaacgagtt
    54961 gtcacttcca gtaaaactat caaactcatc ttgtacttct aacttttctt ttaacatttt
    55021 tatttcttct gatttcattt ttaatttttc actattttta agtgcagcac taacagcttc
    55081 tctagcagtc aaaatttcct tttcagatgt tcttttattt gagtcactta aaatagaatt
    55141 atcaactttg tttatagaag aagtattatt atctgcgtaa actgggatta aattggcagt
    55201 tattatacca acaccacagc caattgcaac tattttgctc atttttttat tcaatacaat
    55261 caccctccat tctatttaaa ctttagcttt caattttggt ttctttatta ttttacgttt
    55321 tatacttcct attttatctg ttatcttacc aaatatttta ggcattttgt ctgaaaactt
    55381 attttcccat ttatcaaata ttaagtatat agtaggtata agcaccaaag ttataagtgt
    55441 agatacagtt agaccaccca ttataactat ggctagtggt tgcatagttt ctccaccttc
    55501 accaagagat agtgctgttg gcaacatacc taaaattgtt gttgctgtag tcattaagac
    55561 tggtcttaat cttgctgcac aaccaattga aaccaaacca attaaatcat tgttacgatt
    55621 tgtttttcta agttgctcta tgtagtcaat aagaactata ccattattaa caacaatacc
    55681 aataagtaag attataccta gcataccgac agtacttaaa ctttgaccac taactagaag
    55741 cgaaagcaca actccaacga atgcaaatgg aacacagaac atgattataa atggtttact
    55801 aaatgattca aactgagcta ccattaccat ataaactaat gctatagcta tgaccattga
    55861 aaatccaaga cttgccatag attcattcat catttctgtt tcaccaccaa cacctataga
    55921 gtaatcttta ggaattacca ttttattcgt tttttctagt gctagttttg tagcagtacc
    55981 agcatctaaa ttgtcaagtg tagcagttat atttacagaa aaatctccat cagtcctaga
    56041 tatagattgt agaccatctg ccatttttat atctgaaaaa gcaccaagag gaacttcttt
    56101 tccagttgca gagtttactt ttagttgctt tatatcttct aagctatcaa tagtatctgc
    56161 tttaaatttt aggtttacat ccactttgta atcatcaata gtagcagtgg ttacggagtc
    56221 accatttatt gctgtacgta acatgcctgc aatttcagca gtatttacac catattcttg
    56281 agccttatgt ttatctatag taaattgagc ttcttgtgtt gtatctgtaa gagaagtttc
    56341 aacatttcta aatcctttta tagagctaaa ttctttttca gcttcttttc ccagtatttt
    56401 taatacatct aaatcaggac ctttcatttc taatgtgaag tctgctcctc cagctccacc
    56461 cataacacta ctgttttgac ttacatttat tttacaatct ggtacagtag ccactctatc
    56521 aattatctct ttttctattt catcagtagt tctttttcta tttttttgag attctaattc
    56581 aattgctata cttgctttac tattatttga atctgatgtc aaagttgtta ttaaagtttt
    56641 aatttctgga atatctgaga cttttttctc agtcatagat acatagtaat cattggattc
    56701 taggctaaga ccttttggaa gttctataga aatagataat tcacccttgt ctgctgcagg
    56761 cataaagtcc atacctataa atcccattcc aaataaggaa gctacaaaaa taagtaaact
    56821 tgatattaca actaagcgtt gatgtctcaa acatacactt aataattttt tatatgcttc
    56881 acttacctta tcaaatatag gactatcttt ttcttgcatc ttttgactat tttttcctct
    56941 gcttaatttt gcaaatacac taggaacgag tgtaattgca cctataaagg agaatgctag
    57001 tgaagctatt agtgttttac ctaatgcccc aaaagttatt ttagctatac cttctgtaaa
    57061 catgataggt aagaatattg ctatcgtggt caatgttgat gcaagtattg catttgttac
    57121 agttcttgtt ccctcaatag cacaatcatc aagactcaat ctgccacttt tcctatattt
    57181 aaatatattt tcaattacaa ctgtagcatt atcaacaacc ataccaactg aaattaccaa
    57241 tgaacttgca gtaataagat ttaaactttc accagtaaag tagagaattg ctatagcacc
    57301 tattatagaa attggtattg caactgcaac aactagtgaa gctcttacac tttttaggaa
    57361 agcaaatatt acaataaatg cgataacagc acttataagc aagttgctca ttacactgtt
    57421 tattgattca cttatgtatg aacttgtatc attagcaatt ttaattttaa attgaggatt
    57481 ttccttattc aattcatcta cgtatttgta tgcactctgc ataactttta cagtgttggc
    57541 atcttgttgt ttagaaatat ctattaccaa actgtctttt ccattgtatc tataaatact
    57601 atctttgtct gcatatccat aatctacact acaaagatta tctaatctaa ctgtatttcc
    57661 tccttttaca ggaatttgaa tctgttttat atcatctagg gattctaatt tatcaattgc
    57721 tcttaaaact attttatcct caccttgagt aattgaacca taagggaatg ttttattact
    57781 agaggataag acacctttta tagtggataa actaactcca taatttgaaa gaacagctgg
    57841 gtcagctatt acattaacct gtgctttttt tccaccttta aggtctgctg aagtaacacc
    57901 atctatggat tctattttag gctgaattac atcttcagcg tattttctta aggcttgtga
    57961 actattttca tttcctccag atatgacaag ttgacctata gcctgtgcat tcatatccag
    58021 ctttaaaact gttggtttag ctgcatcatc tgggaggctg gtctgtaatg catcaacttt
    58081 agacctaaca tcattcatta tttcatccaa attagttcca aattcaaact gagcaactac
    58141 catagaaaca ctttcttgtg aacttgatat agtagatttt acatttgaaa cggcagataa
    58201 actttcatca actttttgac taacttgttc atcaacgtct tgaggaccag cacctgtcca
    58261 agtagtcatg accattacaa ctggtatttc tatatcaggc attagattta ttgacatttt
    58321 tttatatcca ataccaccaa aaactattaa tactaaaaag accattatga ttgtgagagg
    58381 acgtttaacg gaagtctgtg ttaaattcat agtttacttc tccttttcta caggaaatat
    58441 cttagttccg tcagaaataa gacttatccc tccaataatc actgtatctt tatcacttac
    58501 accactttta atttctattt tatcctctgt tacaattcca gtttgaacca agtgtttctt
    58561 tgctatattg tcttttgttg ctatgtatac atatttttta ccattttctt caaatattgc
    58621 ttttttaggt actgtaacag caccattttc tttttcaaca cttaagttta cttgagcaga
    58681 catacctgcc ttaaatttgt tgtttgtatt atttacaagt actttaacag gatataaaga
    58741 tgtctgaggg tcaactactg aaggtacata atctattttt cctaaaatcc tttggtcttc
    58801 aattataaca tctacatttc catctttctt aaatttacct atatctgatt ctgctacatt
    58861 taaatcaact tctagtatat taggattaga aataataaaa gctggttgtg attgtgttat
    58921 catttccttt gaatcaaagt ttttagctgt tattgtacca tctactggag atgttaatgt
    58981 taatttgtct aaattactca ttgctaaatt atatgatact tgagcttggt caagctgttt
    59041 tttagcaagt gctttagctt gaggtatact ttttgaagtt gctgtatcgt atgctctttg
    59101 agcagaatct aactgttgtt ttgtttgctc taaagaaaat tctatttttt taaattggtc
    59161 taaactaatg acttcctgtt cataaagaat cttattattg tcataatttc tttgagactc
    59221 ctcatattga atctttgcat tgtcaagagc tgtttttgcc tgatttactt gtgcctctaa
    59281 tgaaccacca gtggcacttg aataacttgc ttttgctaaa tctagtgagg ctttactttg
    59341 ctttacacta ttttctagct ctggattgct tatactcaat aattcatctc cttttcttac
    59401 attttgccca agttttacat acacttcttg aactgtgcca acagtctgtg cagtaacagc
    59461 tgtttcatct ttaacctttg tagttcctga gaaagaattt gtgttttcaa tttctccacc
    59521 tattgctttt tgaacagaaa ctgctattgg tttctcaggt tcagattctt ctgtttttcc
    59581 acatccaact aaaaacagtg aactacacat taagaaaact attaaatatc ttttcatatc
    59641 tcctcctatt atatattgca cactagcttg ttgtagaatt taaagtgtct tataatttaa
    59701 gtttagtttt agactaatta aaaaacttcc ctttattggc aatttataat tctctaattt
    59761 gattataaat ttataattat tatgagtcaa taactttt
//
LOCUS       DAELJO010000006        59147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELJO010000006 DAELJO010000000
VERSION     DAELJO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.006"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            200..337
                     /gene="rpmH"
                     /locus_tag="KPB88_000300"
     CDS             200..337
                     /gene="rpmH"
                     /locus_tag="KPB88_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG0052614.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            415..759
                     /gene="rnpA"
                     /locus_tag="KPB88_000301"
     CDS             415..759
                     /gene="rnpA"
                     /locus_tag="KPB88_000301"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG0052615.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            791..1042
                     /gene="yidD"
                     /locus_tag="KPB88_000302"
     CDS             791..1042
                     /gene="yidD"
                     /locus_tag="KPB88_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG0052616.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            1074..1781
                     /locus_tag="KPB88_000303"
     CDS             1074..1781
                     /locus_tag="KPB88_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0052617.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1750..2379
                     /locus_tag="KPB88_000304"
     CDS             1750..2379
                     /locus_tag="KPB88_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG0052618.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2491..3870
                     /gene="mnmE"
                     /locus_tag="KPB88_000305"
     CDS             2491..3870
                     /gene="mnmE"
                     /locus_tag="KPB88_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG0052619.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            3968..5860
                     /gene="mnmG"
                     /locus_tag="KPB88_000306"
     CDS             3968..5860
                     /gene="mnmG"
                     /locus_tag="KPB88_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG0052620.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            5853..6572
                     /gene="rsmG"
                     /locus_tag="KPB88_000307"
     CDS             5853..6572
                     /gene="rsmG"
                     /locus_tag="KPB88_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG0052621.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6735..7520
                     /gene="noc"
                     /locus_tag="KPB88_000308"
     CDS             6735..7520
                     /gene="noc"
                     /locus_tag="KPB88_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG0052622.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7655..8428
                     /locus_tag="KPB88_000309"
     CDS             7655..8428
                     /locus_tag="KPB88_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0052623.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8430..9293
                     /locus_tag="KPB88_000310"
     CDS             8430..9293
                     /locus_tag="KPB88_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG0052624.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9326..10468
                     /locus_tag="KPB88_000311"
     CDS             9326..10468
                     /locus_tag="KPB88_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0052625.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10601..11176)
                     /locus_tag="KPB88_000312"
     CDS             complement(10601..11176)
                     /locus_tag="KPB88_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG0052626.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11436..12728
                     /locus_tag="KPB88_000313"
     CDS             11436..12728
                     /locus_tag="KPB88_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0052627.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            12833..13435
                     /gene="yedF"
                     /locus_tag="KPB88_000314"
     CDS             12833..13435
                     /gene="yedF"
                     /locus_tag="KPB88_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG0052628.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELNNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13508..13771
                     /locus_tag="KPB88_000315"
     CDS             13508..13771
                     /locus_tag="KPB88_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG0052629.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            13786..13971
                     /locus_tag="KPB88_000316"
     CDS             13786..13971
                     /locus_tag="KPB88_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG0052630.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14074..15270
                     /locus_tag="KPB88_000317"
     CDS             14074..15270
                     /locus_tag="KPB88_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0052631.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15484..15762
                     /locus_tag="KPB88_000318"
     CDS             15484..15762
                     /locus_tag="KPB88_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG0052632.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            15799..16233
                     /gene="ssb"
                     /locus_tag="KPB88_000319"
     CDS             15799..16233
                     /gene="ssb"
                     /locus_tag="KPB88_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0052633.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16252..16479
                     /locus_tag="KPB88_000320"
     CDS             16252..16479
                     /locus_tag="KPB88_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG0052634.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16622..16942
                     /locus_tag="KPB88_000321"
     CDS             16622..16942
                     /locus_tag="KPB88_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0052635.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            16950..17906
                     /locus_tag="KPB88_000322"
     CDS             16950..17906
                     /locus_tag="KPB88_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG0052636.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            17917..19911
                     /locus_tag="KPB88_000323"
     CDS             17917..19911
                     /locus_tag="KPB88_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG0052637.1"
                     /translation="MSNKQAFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            19912..20361
                     /locus_tag="KPB88_000324"
     CDS             19912..20361
                     /locus_tag="KPB88_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG0052638.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20380..21708
                     /gene="dnaB"
                     /locus_tag="KPB88_000325"
     CDS             20380..21708
                     /gene="dnaB"
                     /locus_tag="KPB88_000325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG0052639.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            21913..22221
                     /locus_tag="KPB88_000326"
     CDS             21913..22221
                     /locus_tag="KPB88_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052640.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22303..23592
                     /locus_tag="KPB88_000327"
     CDS             22303..23592
                     /locus_tag="KPB88_000327"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG0052641.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            23918..23993
                     /locus_tag="KPB88_000328"
     tRNA            23918..23993
                     /locus_tag="KPB88_000328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23951..23953,aa:Lys,seq:ttt)
     gene            24000..24074
                     /locus_tag="KPB88_000329"
     tRNA            24000..24074
                     /locus_tag="KPB88_000329"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24033..24035,aa:Glu,seq:ttc)
     gene            24079..24154
                     /locus_tag="KPB88_000330"
     tRNA            24079..24154
                     /locus_tag="KPB88_000330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24112..24114,aa:Val,seq:tac)
     gene            24188..24262
                     /locus_tag="KPB88_000331"
     tRNA            24188..24262
                     /locus_tag="KPB88_000331"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24220..24222,aa:Thr,seq:tgt)
     gene            complement(24645..25637)
                     /locus_tag="KPB88_000332"
     CDS             complement(24645..25637)
                     /locus_tag="KPB88_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0052642.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25646..26704)
                     /locus_tag="KPB88_000333"
     CDS             complement(25646..26704)
                     /locus_tag="KPB88_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0052643.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            26829..28292
                     /locus_tag="KPB88_000334"
     CDS             26829..28292
                     /locus_tag="KPB88_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG0052644.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28394..29191
                     /locus_tag="KPB88_000335"
     CDS             28394..29191
                     /locus_tag="KPB88_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0052645.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDISNVYEMK"
     gene            29204..31306
                     /locus_tag="KPB88_000336"
     CDS             29204..31306
                     /locus_tag="KPB88_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG0052646.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSILFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31374..31853
                     /gene="rlmH"
                     /locus_tag="KPB88_000337"
     CDS             31374..31853
                     /gene="rlmH"
                     /locus_tag="KPB88_000337"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG0052647.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKYKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32002..32313
                     /locus_tag="KPB88_000338"
     CDS             32002..32313
                     /locus_tag="KPB88_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052648.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32361..32678
                     /locus_tag="KPB88_000339"
     CDS             32361..32678
                     /locus_tag="KPB88_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0052649.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            32891..33592
                     /locus_tag="KPB88_000340"
     CDS             32891..33592
                     /locus_tag="KPB88_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0052650.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            33816..35117
                     /locus_tag="KPB88_000341"
     CDS             33816..35117
                     /locus_tag="KPB88_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0052651.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35254..36348
                     /locus_tag="KPB88_000342"
     CDS             35254..36348
                     /locus_tag="KPB88_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0052652.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKTIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            36350..37483
                     /locus_tag="KPB88_000343"
     CDS             36350..37483
                     /locus_tag="KPB88_000343"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG0052653.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYEVKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            37501..38610
                     /locus_tag="KPB88_000344"
     CDS             37501..38610
                     /locus_tag="KPB88_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG0052654.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            38675..39415
                     /locus_tag="KPB88_000345"
     CDS             38675..39415
                     /locus_tag="KPB88_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG0052655.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNYSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            39420..40412
                     /locus_tag="KPB88_000346"
     CDS             39420..40412
                     /locus_tag="KPB88_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG0052656.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40600..41481)
                     /locus_tag="KPB88_000347"
     CDS             complement(40600..41481)
                     /locus_tag="KPB88_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0052657.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            41754..43142
                     /locus_tag="KPB88_000348"
     CDS             41754..43142
                     /locus_tag="KPB88_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052658.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            43170..44123
                     /locus_tag="KPB88_000349"
     CDS             43170..44123
                     /locus_tag="KPB88_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0052659.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            44183..44407
                     /locus_tag="KPB88_000350"
     CDS             44183..44407
                     /locus_tag="KPB88_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052660.1"
                     /translation="MNEKSSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIVIKLLEIRQAREATNYNIGNG"
     gene            44400..44912
                     /locus_tag="KPB88_000351"
     CDS             44400..44912
                     /locus_tag="KPB88_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG0052661.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLVILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     IFSKDRKKGN"
     gene            44944..45798
                     /locus_tag="KPB88_000352"
     CDS             44944..45798
                     /locus_tag="KPB88_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG0052662.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            46330..47124
                     /locus_tag="KPB88_000353"
     CDS             46330..47124
                     /locus_tag="KPB88_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0052663.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            47228..48034
                     /locus_tag="KPB88_000354"
     CDS             47228..48034
                     /locus_tag="KPB88_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0052664.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            48096..48617
                     /locus_tag="KPB88_000355"
     CDS             48096..48617
                     /locus_tag="KPB88_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0052665.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            49032..51119
                     /locus_tag="KPB88_000356"
     CDS             49032..51119
                     /locus_tag="KPB88_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0052666.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            51134..51421
                     /locus_tag="KPB88_000357"
     CDS             51134..51421
                     /locus_tag="KPB88_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052667.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            51448..52719
                     /locus_tag="KPB88_000358"
     CDS             51448..52719
                     /locus_tag="KPB88_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG0052668.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            52777..53376
                     /locus_tag="KPB88_000359"
     CDS             52777..53376
                     /locus_tag="KPB88_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0052669.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            53381..54286
                     /gene="dapA"
                     /locus_tag="KPB88_000360"
     CDS             53381..54286
                     /gene="dapA"
                     /locus_tag="KPB88_000360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0052670.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            54392..55024
                     /locus_tag="KPB88_000361"
     CDS             54392..55024
                     /locus_tag="KPB88_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG0052671.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            55502..58009
                     /locus_tag="KPB88_000362"
     CDS             55502..58009
                     /locus_tag="KPB88_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0052672.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            58351..>59147
                     /locus_tag="KPB88_000363"
     CDS             58351..>59147
                     /locus_tag="KPB88_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052673.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGHSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGV"
BASE COUNT        23301 a         6386 c        10630 g        18830 t
ORIGIN      
        1 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
       61 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
      121 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
      181 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
      241 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
      301 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
      361 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
      421 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
      481 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
      541 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
      601 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
      661 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
      721 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
      781 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
      841 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
      901 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
      961 tgtaaaaggt atgtatttaa cgataagaag acttttaaaa tgtcatcctt ttcatccagg
     1021 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
     1081 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
     1141 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
     1201 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
     1261 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
     1321 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
     1381 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
     1441 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
     1501 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
     1561 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
     1621 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
     1681 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
     1741 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
     1801 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
     1861 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
     1921 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
     1981 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
     2041 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
     2101 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
     2161 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
     2221 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
     2281 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
     2341 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
     2401 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
     2461 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
     2521 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
     2581 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
     2641 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
     2701 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
     2761 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
     2821 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
     2881 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
     2941 aatcaattag agggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
     3001 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
     3061 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
     3121 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
     3181 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagtcact
     3241 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
     3301 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
     3361 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
     3421 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
     3481 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
     3541 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
     3601 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
     3661 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
     3721 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
     3781 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
     3841 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
     3901 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
     3961 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
     4021 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
     4081 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
     4141 tcaattagtt aaagaaatag atgccttagg cggtcagatg ggattaaata tagacaagac
     4201 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
     4261 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
     4321 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
     4381 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
     4441 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
     4501 ggggtatgca aaatatctta cagatagctt agttgagctt ggtcttagaa tgagaagatt
     4561 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
     4621 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
     4681 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
     4741 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
     4801 ttctatagaa gataaagtag tacgatttag tgataaaaca tcacatcaat tatttataga
     4861 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
     4921 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
     4981 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
     5041 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
     5101 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
     5161 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
     5221 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
     5281 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
     5341 taaaaaagat agatatgaaa ggttcctaaa taaaaaggct gctgtagaga aagaatttga
     5401 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
     5461 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
     5521 ttacgaattg cttgaagaat tagggaaagg cgctggagaa gatgtttcaa gagaagttaa
     5581 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
     5641 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
     5701 agaaggtctt agattagaag ctagacagaa attagatgat ataaaaccta tttcaatagg
     5761 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
     5821 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
     5881 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
     5941 gagagatatt agttgaatgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
     6001 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaaaaatgga tatataaaaa
     6061 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
     6121 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
     6181 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
     6241 aagattttgg taaaaatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
     6301 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
     6361 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
     6421 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
     6481 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
     6541 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
     6601 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
     6661 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
     6721 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
     6781 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
     6841 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
     6901 aactaattgc aggagaaaga agattaagag cagctaaatt agcagagctt aaaaccatac
     6961 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
     7021 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
     7081 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
     7141 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
     7201 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
     7261 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
     7321 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
     7381 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
     7441 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
     7501 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
     7561 cttgtaatat aaattgattt atagacatta tttatactta aaaaacacct attcaacatt
     7621 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
     7681 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
     7741 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
     7801 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
     7861 tattaaagaa gcaataataa gtactgaatt tgagaatgta gatgtagttc catcagctac
     7921 tgaactatca ggtgcagaaa tagagcttac atctaaaact aatagagagt atattttaaa
     7981 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
     8041 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
     8101 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactataa aattagtaaa
     8161 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
     8221 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
     8281 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
     8341 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
     8401 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
     8461 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
     8521 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
     8581 atgaagaaaa gataaaagta ttgtcggatt ctataaaaaa ttatggtgtt ttacaaccaa
     8641 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
     8701 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
     8761 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
     8821 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
     8881 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
     8941 gacaagatgt tatagaaatg atagacagtg gtagaattac agcaggccat ggtaaggctt
     9001 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
     9061 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
     9121 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
     9181 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
     9241 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
     9301 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
     9361 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
     9421 aggtagagca ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
     9481 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
     9541 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
     9601 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
     9661 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
     9721 atcaataaag aataatacta aattgattgt aacaacacat gcgtctaatg tatgtggaac
     9781 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
     9841 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
     9901 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
     9961 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    10021 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    10081 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    10141 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    10201 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    10261 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    10321 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    10381 agtaaggttc agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    10441 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaagg
    10501 ggatatttca aaataaaaat ttgaatcatc tccttttttg tataaaaaag aaaaggggtc
    10561 cctaagaacc tctaatctat ttgataattg ttttaagaag ttactttaag taatctcctt
    10621 cttcaagaac tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt
    10681 caacattcat attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat
    10741 cactacctaa gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac
    10801 catcaatgtc ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt
    10861 tttgaagttc ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa
    10921 ttaaatctgt cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt
    10981 cttttggagt aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt
    11041 ttggttgctc aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt
    11101 gagcattagt acaaccaact gcgaatatag atatagataa tatacttaac actaacatta
    11161 ttttcttatt tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata
    11221 gtcgttatga gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta
    11281 aattaataaa actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt
    11341 acagtgaaaa tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat
    11401 attataaaaa aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa
    11461 agattaaaaa attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga
    11521 taacagcagc acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt
    11581 tggaatatct aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa
    11641 tgcaatcaaa aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa
    11701 atgaattaaa caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg
    11761 atttaatgga gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat
    11821 ctaattataa tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata
    11881 gtgatagtga aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag
    11941 acttttataa aggtgctata agccacttta attttgcaga ggaaattttg tcagagagta
    12001 aatttgagga tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa
    12061 aactagataa tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata
    12121 gaaatgatag aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga
    12181 ttggaaaata tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg
    12241 aggataataa aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa
    12301 taggtgaaac agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg
    12361 atgaagatga atacatgatg tcaggattat ttaagataat agaagcatat atagataaag
    12421 aagattatga tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata
    12481 aaaataaatt caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga
    12541 atgaagtaac tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag
    12601 gcaacaatag aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat
    12661 caaaagaaaa agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aacttagaaa
    12721 taatgtaggg taaataaacc tataagggta tagaaacaat ctataacaag caagataata
    12781 tctatgataa aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat
    12841 aaaaatagat gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt
    12901 ggataaccta gaagaaggtg ttgtagttac aatagttgat aatgaaacag ctaaggagaa
    12961 tatattaaaa cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa
    13021 ttttatatct atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga
    13081 taataaagaa agtgaattaa acaatacatg tatatttata tcttcagata agatgggact
    13141 tggaaatgac gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc
    13201 taaaccttat cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa
    13261 tgaagaaacc atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg
    13321 tggtacttgt ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa
    13381 tatgtatact atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat
    13441 gtatgtttaa taatataaaa ctaacaataa agattaactc aaatagatag tcagaaagga
    13501 ttaagtaatg aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc
    13561 agaaaagtta tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat
    13621 aacagcaagt tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac
    13681 tattttggtt gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa
    13741 tggttcaaaa gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta
    13801 aaaataggtg atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa
    13861 atcataagaa cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg
    13921 cttgatagag agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt
    13981 aacacttaaa aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat
    14041 tgaagaaaac attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg
    14101 caaggattac aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata
    14161 atatcttttg ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa
    14221 gtatcagttg cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa
    14281 ggatatgaac cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat
    14341 gtaaacaaag atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga
    14401 aaagtattca ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct
    14461 ataaacgcat ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga
    14521 atgataatgg aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttac
    14581 gtaatacctg acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa
    14641 ttcatggaaa tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat
    14701 ttaagatttg aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta
    14761 atattcttag gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt
    14821 gcttctccag aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca
    14881 tcaactatat ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat
    14941 gttgacaaaa taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa
    15001 gaagagttcc cagaaggatt agtatttact catccagaag gtggattatt tacatgggta
    15061 gagcttccaa gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt
    15121 gcttatgttg ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt
    15181 aactattcaa atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta
    15241 ttaaaagaag ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag
    15301 ggatggttgt tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact
    15361 ttgtccaaaa tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat
    15421 tttagtctca tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt
    15481 atagtgagaa attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga
    15541 gaggctatac taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt
    15601 gatacttggg gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat
    15661 gtattagtta acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag
    15721 ataaatgaaa acgtaataag acacatgata gttgttgctt aataactggt tttattataa
    15781 aaaataggtg gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct
    15841 gaattaagat acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat
    15901 agagactatg taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg
    15961 ggtaaatctg ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa
    16021 ggatctataa gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa
    16081 gtaagtacta gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga
    16141 gagagtgctc cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc
    16201 caagctatag atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat
    16261 aaaaaaagac gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata
    16321 gattacaaaa gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca
    16381 agaagaatat ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga
    16441 gcgagaaata tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat
    16501 tttttattat atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt
    16561 tatatactta aatatattat tatttatgat ataatattat atattaagga gggttgtaaa
    16621 cgtgaaaata gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa
    16681 aacagaaata ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc
    16741 attagatgaa gcaattcaag atactttagc aaatataata atgataacat atctcttagc
    16801 aaagagatta ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat
    16861 tggtatagat gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa
    16921 acatatggat aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa
    16981 tgtctataat agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag
    17041 gaatatttag tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa
    17101 agtattctct tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt
    17161 cttatgcatt aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc
    17221 taaagagaag ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca
    17281 taatatttgt actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga
    17341 atctattaga tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt
    17401 tttctgcaac tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt
    17461 ttacaaatat gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat
    17521 attatttgag tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt
    17581 ttgctgattt ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag
    17641 tcttatttat tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac
    17701 agctagtttt taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa
    17761 agaaatggat aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt
    17821 tatttggatt tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa
    17881 aggtaagaag ctataaatcc acttaggagg atagaaatga gtaataaaca agcctttaaa
    17941 ttaaatatgc cagaaataaa tttatatata attgttattg gtatttctag tataattttg
    18001 ctttattaca acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt
    18061 cacaattgga gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta
    18121 tctttagata tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt
    18181 attttagaat ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt
    18241 caaaaagatt tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa
    18301 gtattaaatg aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca
    18361 ataatttata atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta
    18421 tactggatag acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat
    18481 gcaatgatgc taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat
    18541 aagagagctt taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca
    18601 aatggtgcat tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag
    18661 ttaaaaaaat tggaagctga gaagttttct attttagata caataagaca tattgattat
    18721 ggaaataatc ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat
    18781 gagaacctaa aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag
    18841 gctgttgtaa aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa
    18901 aagaaaacca aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa
    18961 agtgaccagg tttatataat gggacataaa tatcctgata tggatgctat gggagcagca
    19021 gttggtgttt atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct
    19081 gtaaatgaat ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag
    19141 ttgtttattg gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta
    19201 gttgatactc atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag
    19261 gtagtagtta tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta
    19321 ttccatgaga tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg
    19381 gatgaagatg taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta
    19441 gatactaaaa actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt
    19501 agaaaagttg gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat
    19561 ttcataataa aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta
    19621 gcatattcaa gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag
    19681 ttattaaata tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata
    19741 tttataagtg cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga
    19801 ggcgggggac acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct
    19861 tataagatgg taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt
    19921 atattattaa aagacgtaaa gggaactggt aaaaagggag aaatgaaaga agtaagtgat
    19981 ggatatgcaa gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca
    20041 ataaaagaat taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa
    20101 gaagcagttt tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca
    20161 ggagaaggtg gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag
    20221 aaacaaaaag acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct
    20281 ttaggttcaa cttttgtaga aataaaaata catcaaaaag taactactaa aataagagtg
    20341 gatgtaaaag aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt
    20401 cctcctcata gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat
    20461 gctataataa cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa
    20521 ataatatatg aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca
    20581 ttaacagaag aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt
    20641 acaagtcttt ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta
    20701 aaagaaaagt ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga
    20761 tatagtggag caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat
    20821 atatcacagg agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc
    20881 tatgatatga tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt
    20941 tttaaagatt taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca
    21001 gctagaccag caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta
    21061 aaaggtgatg cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa
    21121 cgtatgttat cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat
    21181 gataatgatt ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac
    21241 atagatgata ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa
    21301 atagaaaaag gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt
    21361 aataatgaaa gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca
    21421 aaagaattaa actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga
    21481 gcagaccata gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca
    21541 gatatagtta tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat
    21601 ataggagaag taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt
    21661 tggcttggtg aagttcaaag atttggggat aagttgagag acctataatc taacgaatat
    21721 ccacagaacg tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca
    21781 aacattgaaa tttagctaaa aaactatcca cactatccac agagagcctg ttgataagtg
    21841 tgaataagta gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt
    21901 aggaggaata atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta
    21961 cttgatgaaa taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt
    22021 tatgctaata atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaac
    22081 ataattaaca tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt
    22141 ataaaatatg aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg
    22201 ttaaatattg gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta
    22261 agtaaagtgt tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt
    22321 gttggttctc aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa
    22381 gcagatgtag tagtaagagg gcaaggtgga aataatgcag gacatacatt agttgtagaa
    22441 ggtaaaaaat acgcattaca tctaattcca tctggagttt taaatccaaa tacagtgaat
    22501 ataatcggaa atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt
    22561 aaaacggata atataagtac agaaaatata aaaataagtg atagagcaca tgtcatattc
    22621 ccatatcata aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt
    22681 acaacaaaaa aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga
    22741 atatgtgact taatggataa agataaattt gctataaaat taaaagctca aatagatgct
    22801 aaaaatgaaa tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata
    22861 tataacgaat atctaggata tgcagaacaa ataagaaagt atgtggcaga tacgtctgtt
    22921 attgtatatg atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact
    22981 ttattagatt tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt
    23041 ggatttgcaa ttggtgcagg tataggacca aatatgataa aagacgttgt tggtatagta
    23101 aaagcatata ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact
    23161 ggagataaga taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga
    23221 tgtgggtggt ttgatgcagt tatagttaag tatgcagcta gagttaatgg attgactagt
    23281 atttcattta tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct
    23341 tataaaatgg gcgataagat aataacggat ttcccagctt cattagatga tttagcaaaa
    23401 tgtgaacctg tatatgaaga attagatgga tggaatgaag atataactca aatagataac
    23461 tttgatgacc ttccagaaaa tgcaaagaaa tatgtagcaa aaatagaaga actagttgga
    23521 gtaagtgtag atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat
    23581 atatttgctt aatattttat caattaaata atttaaaata gttgaagaag agtatctcaa
    23641 gataatgttt atagttatta gagatgctct tttatatttg acttacattg attttttatt
    23701 aagttaaagc tacttataca tattctaact gaaattaaag ttgaatttat gtagatacat
    23761 ataggttttt taaaaagaat tttgttgaaa ataaatggct aaagatgatt ttaattatat
    23821 tctaaaaaaa gtaaaaaaaa ctattgacta aaaattatat agacgctata atataacttg
    23881 tgagtgaaag aaacgacgaa aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga
    23941 gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg
    24001 gctcgttggt caagaggtta agacaccgcc ctttcacggc ggtaacaggg gttcaattcc
    24061 cctacgagtc accagtatgg gactatagct cagctgggag agcacctgcc ttacaagcag
    24121 ggggtcacag gttcgagccc tgttagtccc accaaatttt gaattagaga taaatactag
    24181 attatatgct ggtgtggctc aacggtagag cagctgactt gtaatcagca ggttgtaggt
    24241 tcgattccta tcaccagctc cacaaagtac ataaattcaa attaatataa ttttctatgt
    24301 tacaggatac ttttaagtat ctttttttaa tttcattttt attgtattaa aaatgaaatt
    24361 aaacatacaa caaaattttg aattacttgc aatgtgatag ttttaaatat aaaatttaga
    24421 atcattctag catagtatta tgtaatatag caaatttaat ttatagtatc aataaatata
    24481 gactactaag aatgtaaatg atttgttaga ttaagataca ttagatatgt aaatagaaaa
    24541 ataaaaagta tgttttaatt tatgtgatat aagttttaaa cagagaagca agtttgctct
    24601 tacctctcta tttaaaaata aaaaaatgtt ttttaagggc tattttattt aaatctttcc
    24661 catctcaaat catctccagt aaattctaaa atcctaaact tatctaatat tctagagaga
    24721 gttctttgag tgtaagtatt tcctatttga gatggtgtta agttggttga aataatgatt
    24781 tttttaccag caacaagtct tgtatttaca atattgaaga tttctccact agtaaaagaa
    24841 ttattaagtt cagttccaag gtcatctatt attaataaat cacaatcaaa caagttttta
    24901 taattatctt cacttatttg attatttgtg tctcttctaa atttataatc ttcaagaata
    24961 tctaaaattc taaaagaagt ttgatagatt acaacattgc ctttatctaa caattccttt
    25021 gcaatacaat tgcacatata ggtttttcct agtccagtag aaccataaaa cagtagattt
    25081 tcactattat ccttcttaaa atcatgaaca aaattttcgc agatacttaa attatttagc
    25141 atattttctc taggagatat ttcaccatca gagcctttct taggagaaaa tatattcaag
    25201 ttaaaattcg aaaaattttc ctgacttaaa atcctatcta aattagacat tttataagct
    25261 tcatttacaa tttcttgttt gaggcaagaa catttttctc cattaggtaa aaatccttta
    25321 tctttacaag aaatacattg aaactttatt tccaagtaat caagaggtat gttactttca
    25381 gctaaaagtc tttctttctt tacttttaaa gattctatat tctctttagt cttctttaca
    25441 atttcatctt tagacttagg atttaaaagt actatcttag ctaaactgag acctatttta
    25501 gaaatttcat catctataga ttttatctca gggattctat tatatacctc attttttcta
    25561 tgttctaata agagttcatt atcatctctt ctcttagcat atttagcaag tatttttctt
    25621 attttatcct cattcattta atcacctact taaacttttc tttttgactt tttttaataa
    25681 tctcatcgag ctcatcagag gtatactgag tgaatgtttc gttgaaatta tgaaacttag
    25741 tttttttgta agtattgttg ttttgagtag tattaatctg cttcttatta atattctcct
    25801 taactatacg ttcttcctct ttttgcttta aatcatttaa attttttata tttttttcat
    25861 tccaattttt aattatacca tttatataag atatacttgg gtttgaaata ttcttagact
    25921 tagaacatgc atttattatc aaatctatat ccatattaaa tttatcaaac caagtgtcca
    25981 taagttcttt ttctgatttt gatggttgtc ttgaaaatcc caattcattg aaaatcgttt
    26041 tatataaaat atatcgttca cttcttacca aaaagctttc ttcaacatct tttggcgtat
    26101 ataaatttga atcataccaa tttctaataa tgccttcaat gtatttaact ggctttgatg
    26161 taccagtttt atctttgacg tattcatatg cgcaaagtat catatcgggg ctcatattgt
    26221 atttattcat tatatccata atactcatct tttcgcttgg gtcgagatat cgcccaacta
    26281 ttttatttat agaattaaac atttttctaa tacttggatt ttcagaagta gatacaacct
    26341 tatctgtatt agattttata gatgaattat tgcttaatat attctcaata tagaaatttt
    26401 ttaaatctaa aaattctatt gaataattaa aattatcata ttctccattg tcatgtattt
    26461 ttataatctt cttttcttcc cagaatttcc aagcagataa cacatcagac aatggtatat
    26521 ttaggttttt ggctattgaa ttattgtcga acttaggatt agaagtaata tcacatgctt
    26581 gtctatatcc taaaagataa acttttacgt ataacccgtc cgccattggc ataaatatat
    26641 ctataaagat attgggaata gtgatttcac ctaaatctat ttcattgctt tctttaaaaa
    26701 acattttatc acctcagatt aaattatatc attttttata ttatatttgt ctgtaaacaa
    26761 ataaaaggtc acaagattag catgtaaata ttttctaaaa gtattcattt tgaaaaggag
    26821 gagatatttt ggttataaaa ttcaaccaaa aagtaagaag aacattatca gtagcgattc
    26881 tatttatggt attaataata ggttgcttta tagaaaagga tgacagttta gagacataca
    26941 aagaaaaaac taattcaaaa ataaatcagt ataatatgga tgttatattt gatgatgaga
    27001 ctaagaggtt aatgtgcaat cagaatgttg actacataaa taatacaaaa tcaaacttag
    27061 ataaaatata tttccatata tatccgaatg ctttttccaa gaaagatttt gctccttttg
    27121 aaaaaagtga aatgacaagg gcttatccta atggatttaa cgaaggatat atagatataa
    27181 agaatatatt aaataataat agtaaaatgg agtataaaat taaaggagac aaaaatgaca
    27241 tattagaaat agaattaggg aaagagttaa aaccaaatga aagaatgtca ttagatataa
    27301 aatataacgt aaaaatacca aattcaattg gaagatttgg atatggagaa aatactataa
    27361 atgttactaa ttggtttcct atagcttgtg taaatgatga aaggggatgg aatttaaaga
    27421 gttatgaaac tattggcgac ccattttata gtgagactag cgattttcat gtgaaactat
    27481 tgattccaaa taaatataaa attgctcata caggaaaact tattgatgat aaacatgata
    27541 ataagaaaat gctatatgaa atagaagcgg aaaacgttag agattttgca tttatactaa
    27601 gtagcaaatt taatgtaaac aaagtagatt caaagggagt agctatcaat acatataatt
    27661 taaataaaaa gctttctaaa aaagcaactg aagtggcaaa agattcaata aatatattta
    27721 gtgaattatt tggaaagtat ccttacaaag aattttctgt tgtagcaagt gactttttta
    27781 taggggggat ggaataccct atgcttgtta tgatagacca aagtttatat aatgaaaaaa
    27841 atgaattttt actagaatat gttattgccc atgagacagc acatcaatgg tggtattctg
    27901 ctgttggaaa tgatgaaata agtgaaccct ggttagatga agcacttact gaatattcta
    27961 caatagttta ttttgaacaa aagtatggta aagatatggc aaacaagctt ataaaaacta
    28021 tggagataca gacaaaaagt tatttgagcg aaaatatatt taagccagca aatgagtata
    28081 aaaattctac tgaatacagc ttgaatgttt atacaaaagg cgcaatagca tttaatgaaa
    28141 taagaaaaga agttggagat aaagtattct ttgagacttt aaatgagtac tataataagt
    28201 ataaacataa aaatgctaat ggaagagctt ttgtggaact atggaatagt aaaggtgtag
    28261 atataaataa gattataagt gaatgtaagt aaacagtgaa tgtaagtaaa ttttgtgcag
    28321 agtattaaat tttatttaat actctgtttt tttggtataa tataatttat aaggcagaaa
    28381 aagaggtgaa ggaatgctaa agtattgttc agtagggagt ggtagtagtg gtaattgcca
    28441 ctatataggg tataaaaata ctaatatact agtagatgca ggattaagtg gcaagagaat
    28501 aactacaggt cttaaagaca ttggtgtaga tgcagataaa ttaaaaggaa tatttataac
    28561 acatgagcat gttgaccata taaaaggagc aggtatatta tcaagaaagt ttgatatacc
    28621 agtatttgct aatataaaaa catggtgttc tatgaaggac aaattaggtg acatcaaaga
    28681 taaaaatatg aaagtatttg aaaatgataa aacctactca ttaggtgata tcatagtaag
    28741 acctttttca atagaacatg atgcgtctga cccagttgga tataattttt atacagagag
    28801 tgatgaaaaa atgtctatag ctactgatat aggatgtata actcaaaata taaaaaaaca
    28861 tctatataaa tctaaattag tagtattgga atcaaattat gaccctaata tgcttatgat
    28921 gggttcatat acatatgctt taaagaaaag agtaatgtca agtactggtc atttatccaa
    28981 tgaagatgca gctaatttct gcgttgaact tattaatgaa ggtacagaat ctatattatt
    29041 agcacatttg agtaaagaaa ataattttcc tgagttagcg tatgagactt caaaaggagt
    29101 attggcatca aatgatatag tagttggaca agatgtaaaa ctagatgtac tatcacgaaa
    29161 tgatatttct aatgtttatg agatgaagta gaggtgaata actttgctaa agagaaattt
    29221 tgaaaatgtt gaggataata aaattttata ctctataaag cagggcatga ttttagcgat
    29281 acctgctatt atgactggtt caacggctct tgtaattttg aatcttccaa taaaggctta
    29341 tcaagaatat ttatcaggct tgtttaatgg agaggttaca aatattctta actttataaa
    29401 tgattctact ttaggaatta tttcattaat aatattatta acaataagct atagttatgg
    29461 aaaaatatat gggagcaagt atacagtact tgttcctata gttgcaatgt gctctttttt
    29521 agtattttcg catggtaatg agcttaattc ttatatagaa atatttaaga caaagtggtt
    29581 atttacatct ataattgttt ctatgacatc ttctatattg tttgtaaaac ttacagaatc
    29641 ttttgttaca agtgtaaaat ttcatacaga aggggctgat gcagacttta atatggtagt
    29701 ttcggctata gttcctttca taatagtagt atttttgttt tctattttta gagtgataat
    29761 gataagtcta attggcagtt caaactttca agatatattt tataatttat tttcaaatgt
    29821 ttttaataaa atgggaacaa accttatgag tgctctgttg tttatttttt taatgcactt
    29881 tatgtggttt tttggaattc atggtagtaa tgtattagat accgtcgcaa aaaatttatt
    29941 tgaaaatagt atggcaatta acataaattt agttaataat aatcaattac caacagaaat
    30001 ttttactaag accttttttg atacaatggt tttattggga ggatgtggct ctttactatg
    30061 tttagtaatt gcaatttttc ttagtgaaaa acgtataaat gtaagaaagt tagctaaaat
    30121 tgcttcaata ccagctcttt ttaatatcaa tgagatgcta gttttcggta taccaattgt
    30181 ttttaactat attatgtttg taccttttgt aattactcct ataatcttaa ccattacaag
    30241 ttatgtggct atgtctacag gtattgtacc atgtacagtt tcagctgtag aatggacctc
    30301 acctatattt ttaagtggat atatgtctac aggttcaata agaggtagtt tgttgcaagt
    30361 atttaattta tgtgttggtg ttatgattta tattccgttt ataaaaatgt cgcaaaatag
    30421 atatacatat atgtttaaaa ataatataga aaatttaaca aacttattaa aaaggtatga
    30481 attaacaggt gaacagccta atcttctaaa ccataatggt aaaataggtt caatttcaaa
    30541 aatgttggca tcagacttga agtttgctat gaaaagagga gaaatagagc ttttttatca
    30601 accacaagtc aattataatg gaaatgttgt gggagcagag ggattactta gatggaaaca
    30661 ttcaatagga gggttcattt atccaccact agttataatt ttggctaaag aagaagggtt
    30721 cttagatgag cttggagagt atattattaa taaagcatgt attgatttaa agaagagtga
    30781 aaagctattg agaaatcctg ttaagttctc tgtaaatata tctccagacc aacttgatga
    30841 ccctaagtta ccagagcaaa taaaaaatat cattagtagt aatgatataa atcctaatat
    30901 gttaggtata gaaataacag agcaaattgc attatcaggt tcacaaataa ttattgaacg
    30961 tatcaatgcg atacataact tgggaataaa gttaattatg gatgattttg ggatggggca
    31021 tagctctctt atatatcttc aaaataataa ctttgatata gttaaattgg atggttcatt
    31081 agttaaagag atacttacaa ataaaagaag tagtgaaatt ataatgacta ttgtaaatct
    31141 ttcaaaaaat ttagattttg atatagttgt tgagtatgtt gaaaatatag accaaaggga
    31201 taagttatat ggtttaggat gtgaaatata tcaaggatat tattatagca aagcaatacc
    31261 ttttgaagag tttgtagaat atgcaaatca agaaagagag atataatttt ataagagctt
    31321 gtaaattcta gtaatgcata gatttgtaat aaaattaagt ttaggagaaa tatatgaata
    31381 taagtatagt agtagtaggt aaaattaaag aaaaatattt aaaattaggt attgatgagt
    31441 ttaagaaaag gctttctaag tattgtaagt tagaaattat agagttggat gatgaaaaag
    31501 ctccagagaa tttgagtgtt aaggaaatgg aaattataaa atacaaagaa ggaaagaaaa
    31561 ttttagggaa gataaagcat aatagttatg ttatagcctt ggctattgat ggtaagggtt
    31621 tatcctctga agagcttgct aagactatga gtgatttagc agttagaggt aatagtagtc
    31681 tctgttttat aataggaggt tctttagggc tttctgatga ggttttagga agggctgatt
    31741 ataggttatc cttttctaag atgacttttc ctcatcagat gatgagattg atattgttag
    31801 aacagattta tagggcttat aggataaata atggagaacc gtatcataaa taaatgatac
    31861 ggtttttttt agtttgcaac ttaataatat taaaataaca aaaaagtatt gaaagtatat
    31921 tgcatttaag gtagaattaa ataaaatatt ataacattat aatgtttata tagttgttaa
    31981 taaaaatttg gaggaaaata tatgataaaa ataatgttgg cctgttcagc agggatgtct
    32041 acaagtttat tagttactaa aatggaaagt gcagctaaag aaaatggtat agagtcgcaa
    32101 atttgggcaa taccagaatc aactatacaa aatgaaatag aaaaatgtga tgtattatta
    32161 ctaggaccac aagttagata tgtattacct aaggctcaag aaattgctaa gccatataat
    32221 ataccagtag aagttataaa tatgatgcac tatggaacag ttaatggaga agctgtttta
    32281 aatagagcga tagaacttaa caacactaag taatataaat tataatgtat ttttaatata
    32341 taaattggga gaatcaaagg atggatgaaa agattataga aatatctttt aatataatag
    32401 gttatgcagg tgaaggtaaa ggattagctt ttgaagcaat aaaagaggct aagaatggaa
    32461 atatagaaaa agcaaaagaa ttattaaaag agtctaggga agttataaat aaagcacata
    32521 gatatcaaac agaattaata caaaacgaag ctagtggaaa caggactgaa ataagtgtca
    32581 tacttataca tgcacaggac cacctaatga atggtatgaa ttttcaacag ttagctgaag
    32641 aaatcgttga tttacatttg aaattacaag ataaataaaa attaaagata ataaattaag
    32701 agagttctta aaagatacat tatgtgtagc acactaattg ttagattatt gattcaatat
    32761 ttagtgagta atacattagg attattttga ggactctttt tatatttagt ttaaagttaa
    32821 taaatattta ttattaataa tataagatta tatgttataa tattataaca tataatttaa
    32881 gaggtgaatc atgttaagaa atcaaccatt atatatacaa gttgtagata tattaaagca
    32941 aaaaatatta aataacgaat ataaaattga tgaaaatatt ccaacagaaa gggagttaga
    33001 gcaagagttt ggagtcagta aaattactat tagaaaagct attgagatat tagaatcaga
    33061 agggtatgta gagaaaaaaa gtggaaaagg gacaaaagta ataagtaata gcctttttaa
    33121 taagctatca aaagcagagt ctttttcaag tatactttta tctaaaggtc ataagttatc
    33181 taaagagact atttcaattg aaaaaataat aaatgataca aattcacaag tatttagtat
    33241 atttggagaa aaatgtagca agattactag aatgtattat ttagatagta agccatatat
    33301 attttttact cattatattc cttttgatga aaatctaact gttgaaagtt ttaataatga
    33361 aagttcttta tatatgtact tatataggag aaatataaat ataactagat ttgaagatga
    33421 attttttata gaagaatcag aactagaggt atcaaaggct ttaaatctag ctaaagacat
    33481 attattagga agaaaaagaa agacctatga tgagaatgat aatatagttg agctatcatt
    33541 tgcaagatat aatacagaag tacaaaacta tgtaattaaa tacgaaatat aataagaatc
    33601 ctgagtttat tgaaatgata atatgctcag gatttttgtt tagttgtaaa aataaaaaat
    33661 attataacat tataatgttt acaaccaaac attataatgt tataatattt ttaataaaca
    33721 agtgatatta tcaattacat aaaaattagt ttatgttatg aaatcgttat tgcattataa
    33781 ataaaggata atatcaataa tacaggggga taactatgga aaaattcgca agttttttag
    33841 agcgcaagat tatgcctgta gcagcgaaga taagtaatca gagacatatg agagctgtaa
    33901 gagcaggtat aatagcaaca ttaccactaa caatagtggg gtcatttttc acaatattat
    33961 taaacatacc tattgatgct tatatggatt ttatagctcc atttaaagca atattagata
    34021 taccatttag atatactgta ggatttttat ctctatatgc tacttttggg atagcatcat
    34081 cacttgctaa aagctacgat ttagatacaa caggtgttgg tatgcttgca gttatggcat
    34141 ttttagtttc tacaatagtt ccaactcaag tagttgaacc agtaggtgga gttatagaag
    34201 ctggtagatg gatggatatg gcaaaactta attcgccatc attatttgga gctatcgtaa
    34261 catcgttaat tacagttgaa atatatcgtt ttatgaagga aaagaatatt acaattaaga
    34321 tgccagcagg agttccagta gaggtttcaa attcatttac tgcattatta ccgacagcag
    34381 taattttaat gttattctgg gtaataagac atattttagg atttgatata agtgttgcac
    34441 taagcgctat gttatcacct ttaaaaggat tcttatcagg aaatagttta ggtggtgggt
    34501 tactgacagt attcctaata actttattct gggtacttgg aatacatggt ccagctataa
    34561 tggggccagt aataagacct atatgggaca tgtctatagc agaaaatata actgcattca
    34621 cagaaggagt atcagctcat gcaatgccaa acatatttac agaacaattt ttacagtggt
    34681 atgtatggat agggggagca ggggctactc ttgcattagt tgtaatgttt atgttttcta
    34741 agtcacaata tctaaaaagt ttaggtagat taggattttt accagggtta ttcaacataa
    34801 atgaacctat aatatttgga gcaccaatag ttatgaatcc aatactaggg ataccattta
    34861 taattggacc tataataatg acaattctat catatgttct tactattaca ggggttatac
    34921 ctatgatggt agcaagatta ccatttgcaa tgccagcacc aatagcagca gtaatgagta
    34981 ctaactggag tatactagca ggtatcttag tattaatcaa ctttgtgata tcatttgctg
    35041 tttattatcc attcttcaaa gtttttgaaa aacaacaatt acaaagagaa caagaagaaa
    35101 tgagtgaaaa tttataaaaa tacatagata tagaggtttt gtactatata aaatatagaa
    35161 aaagcttata agttattaag ggtgaaggta ggaggaatga cctttgcctt taattttata
    35221 tattttaaat aatgtaattt gaggaggaga aagatggaaa gaagattagg aatatcagta
    35281 tatccagagc atagtaacat ggaagatgat aaggcatata taaaaatggc ttcaaaatac
    35341 ggatttaaaa gaatatttat gtgcatgctt tcaattacaa gacctaagga agaagttaaa
    35401 aaacatttta aagaaattat aacctatgca agagaattag gatttgaagt aatccttgat
    35461 atagctccaa acatatttga tatattacaa atatcttatg atgatttaac attttttagt
    35521 gagttaaatg ctagtggaat aaggcttgac tatggatatg atggaagtaa agaagctatg
    35581 atttctttca atccatatga tttaaaagtg gaactaaata tgagtaatga tgtagcatac
    35641 ttggataata taattactta caagccaaat gtaaataata tatatggttg tcacaatttt
    35701 tatcctcaaa ggggtacagg cttgccatat gatttttttg taagatgttc aaatagattt
    35761 aaaaagtatg gaataagaac tgctgctttt gttaattcaa aatacgcaac aatagggcct
    35821 tggaatataa atgatgggct gtgtacgctt gaaatgcata gaaacttaga tattgctaca
    35881 gcaactaagc acttattcgc aacaggattg attgatgatg taattgtagg gaatgcatat
    35941 gcaagtgaag atgaattaaa aagaatgtct gaagtaaatg aataccaagt tgtatttaat
    36001 gtagtagctg aagaaaaaac ttctgaagta gaaaagacaa taatggtaaa tgagcaacac
    36061 tttagaagag gagatataac taatcaggta gcaagatcta cagaagtaag aaaaaaatat
    36121 gctaaagaaa atttccctaa acatgataat aagaacctct ttaaaagagg agatgtagtg
    36181 attggtaata atgactttgg aaagtataaa ggggaattac agttgatact tcaagattgt
    36241 acagatgaaa gaaaaaataa ggtgggtaga ataaaggaag acgaaataat acttttagac
    36301 tttattgaac cttggagtaa atttaagatt attttagagg gagagtaaag tgaagataga
    36361 catattaata aaatgtggaa aaacagtaga taaaagtata attgatatag ctatattaga
    36421 cgataaaata atagaagtaa aaaatcagat agatgattct aagtatcagg caaaacaaat
    36481 tataaactta gatggagaga aatttattag tgctggttgg atagatatac atacgcattg
    36541 ctacgaaaga ctagaccttt ataaggatta tccagatgag gttggaataa aaagtggagt
    36601 tactactgta gtagatgcag gaacaacagg agctttagat ataggtaaat tttatgaaga
    36661 tactaaaaaa tataaaacaa atgtatatgc tttaataaat attgctaagc agggaataac
    36721 atctcaagat gaactttcaa gtatgatgaa tattgatgaa tatgaagtta aaagagcagt
    36781 taaaaaatac aaagattttg ttgttggaat caaagctaga atgagtaaaa gtgttgtaat
    36841 ttcaaatgat gttgaacctt taaaagtagc taaaagaata aaaaatgaac ttaatctacc
    36901 tatgatggtt cactttggga gttctcctcc aacgatagag gatatatttg actatatgga
    36961 aaaaggagat atattaaccc atatatataa tggaaagcca aatggaatac ttagaggcaa
    37021 tgaggttaaa aaagaaatca tagaagctag agaaagaggt ataatccttg atgtaggtca
    37081 tggaaccgaa agttttagta tggatattgc aatgaaatct aaggatgcag gaatttttcc
    37141 agacactata agtacagata tatatattaa aaatagaata aatggtccag tgtataatct
    37201 atctacaaca atggaaaagt ttatatatat gggctattca cttgaagata taatagataa
    37261 agttactaaa aatgcagcag atgcaataag tttaaaaaat aaaggtttaa taaaagaagg
    37321 atatgatgct gatttaacta tatttgatgt ggttaatgaa gaaaaagaac ttcaagattc
    37381 tttgaataag tcagtaatca caagtacgtc tataaagcca agagcagtag tagtcaatgg
    37441 agaatattta aatataggta agtctatagg agagaatgag tagaagggag aaactattgt
    37501 atgagtagta tatttgaaaa atactctgta agacgtgtta taaatgcttc aggcaaaatg
    37561 actatactag gagtatcaac agtagataag gaagttattg aagcaatagg tgaagggtgt
    37621 aaaaatttct ttataataga agaacttata aataaaacag gtgaatatat agcaaattta
    37681 ttagggtgtg aaagtgcact aatagtttct agtgcatctg ctgggattgc acagagtgtg
    37741 ggagcaatga tagcaaaaga tgatatgagt ctagtttaca atgtaaataa tcctaagaaa
    37801 caagttaaaa gggaaataat aattccaaag ggacataatg ttgattatgg agttccagta
    37861 gaagtaatga taagacttgg aggaggagaa gttgttgagg ccggttatgc taatatgtgt
    37921 aacaaggaac atataataag agagataaat gaaaatacag cagcaattat gtatattaaa
    37981 tctcatcatt gtgttcaaaa gagtatgcta agtgtattag atgcagtaca agttgctaaa
    38041 gaatataatt tacctttgat agtagatgca gcagcagaag aagatttaaa tgtatattat
    38101 gaattgggag cagacctagt gatatacagt ggtgctaagg ctatagaggg tcctagctca
    38161 gggttagtaa taggtaagaa aaaatacata gataatgtaa agcttcaatc aaagggtata
    38221 ggaagagcta tgaaaatagg aaaagaaaat attgtaggtc taacatgtgc aattgagaga
    38281 tatataagta atgataaagt aactttaaaa gaaatggagg acaagctaaa tccatttata
    38341 gacaagataa atactataaa gggagtgagt gctagtataa ctagggattc agcaggtaga
    38401 gaaatacttc gtggagaaat tgactttaat gaagatataa taaataagtc aacacaacaa
    38461 attataaatg aacttagaag tggagagata gctatataca ctagagacta taaggctaat
    38521 gaaggcaaga ttgagattga tataagaagt gtaactggaa gtgatttaga tactatttat
    38581 aataggataa aaaatatagt agaaagttag agtgtagagt aagtgacatt ataatgattt
    38641 aataaattaa attttaccaa tggaggggat aaatatgaat tgtataccac aatattataa
    38701 aaatagagtt tgtttaaatg ttcttgcagg aagcataaaa aatgctaaag aagtatatga
    38761 tgcttgtgac gggcatgtat tgataggagt actatcaaag aattacagta ctgtagaagc
    38821 agctattgaa gatatgaaaa aatactcaaa ggaaatagat aatgcaatat ctgtggggct
    38881 tggagctgga gacccaaatc agtcaaatat ggtatctcag atttctaaag aacttcagcc
    38941 acaacatata aatcaagtgt ttacaggtgt ggctacgtct agagcattgc ttgggcaaaa
    39001 ttatagtata ataaatggat tagtttctcc aactggtaaa ccaggatttg taaaaatatc
    39061 cacaggtcca ttaagttcaa aagaaaaaga tggtatagtt cctatagaaa cagcaatagc
    39121 tatgatgaaa gatatgggag gaagctcaat taagtttttt ccaatgggag ggcttaaaac
    39181 aaaggatgaa tatatagaag ttgcaaaagc ttgtgcaaag cataattttt atttagaacc
    39241 aacaggaggt atagacttag ataacttcaa agagatagtt caaattgcac ttgatgctgg
    39301 tgtagaaaaa gttatacctc atgtttatac atcaataatt gataaagaaa caggtgaaac
    39361 gaaagtggaa gatataggag tactttataa aataatgaaa gaattattgg gatagattta
    39421 tgaatatatt agcttttggt gaaataatga tgagactttc agtacctgac tacaagttct
    39481 taacacaaac caatgaacta aattatataa ttacaggcac gggattaaat atattatctg
    39541 gacttaaaaa ctttggatac aatacctata tgttgacaaa gttgcctaat aataatgtag
    39601 gtaaagcatc cagtgcaaat ataagaaagc ttggagtaaa agatgacttt atcacatatg
    39661 gagggaatca tataggagtg tattttcttg aaaatgggta tggagaaaga ccttctgaag
    39721 tgacttattt gaataggtta aatagttcat tttgtgagag taaattacaa gattatgata
    39781 ttgataaatg tttagaagaa atggatatag tccatatttg tggtattgct atgcaactta
    39841 cagattcagt taaagagatt gctttagaac tagctagaaa atcttatgaa aaggggataa
    39901 aagtattctt tgattttaat tttagacaat cattaaatcc agaaaggact tatgatgatt
    39961 tagtaaatga ttataagaaa atactaccat attgtcatgg gctgtttgct agttatagag
    40021 atataaataa gatacttaat atagatagtc taaatataaa atctagttca gaattgatta
    40081 aaaaagagta tgatataggt attgtagctg gaactatgag aagatttgat gatatgaata
    40141 atagatttat aaagggatat atatcaaatg atgatggtta ttttgaaagt agggagtata
    40201 agttagaaat atatgacaga gttggtgctg gggatggata tgtggcagga attatatatt
    40261 catatttgga aaatatgaag agtgaagata tagttgagtt tggggttgct agttcggtgt
    40321 tagctcatac aacttatggg gataattctt tagtaagcac aggtcatgta cttagactga
    40381 tgaacaatga aaaaatagat atattgagat aatacatttg tatataataa aatttttatc
    40441 ttttttagac acatttttga cttgcttata tttgttaaac agagttattt ttaacgatta
    40501 taaatattaa attaaaagtt aaataataaa atagtagaaa atagcattaa ctatagaaaa
    40561 aatggttaat gctattttta tcgttaatat tgattaattt tatattatag aaatgaactt
    40621 ttctaatgct ggatttgtat tttttttatt ccatacaact acaacttcaa ttctattttc
    40681 tacttctttt ataggtacta ttgcaatatc acgttctatc attgtatctg taacacaaaa
    40741 tgaaggtaaa atggatattc ccatgttaga agataccaat gattcaatag tattaagatt
    40801 atttgaacgt tgtaccacat taggtacaaa ccctgcttca cgatatctat tctgtatcat
    40861 tgacttttgt ccatactcat cccctgtttc ttttacaaaa ataaatttat catgttgtaa
    40921 ttcaattaaa tcgatagaat cctcattgta aagtggatga ccaggaggta aaagagctac
    40981 caaagaatat ctttctacag ttttatattc aaaatctttt aatggttgat tttctggatt
    41041 aataacaaaa gcgaggtcca tagaattatt ttttactaag tttaaaagtt cggttatctc
    41101 attagtacaa atatccatag atatattagg gtatgtgtta gaaaattctt ttaaatattg
    41161 ttgaagtttg tttttttcat atcctattaa ggttccaatg ctcaaagttc caaaaaaacc
    41221 atgtgcaaat tgttgtgttt ttgcaatagc atcattgata ttttttatta tagtacgtgc
    41281 ctcagataaa aaaacttttc cagctggtgt tagttcaaca tgacgtttac ttcttgtaaa
    41341 tagagttaca tttatttgtt cttctaaagc ttttatttgc tgtgtaacag cagtttgaga
    41401 aatataaaat ttttctgctg ttttggtaaa acttagattt tctgctacag atataaaata
    41461 ttctaattgt ttagtattca aataatcacc cctatttaaa cttgattgtt tgtaaacctt
    41521 atttatctat aattaaagct tattatagta taagaaaatt tacttttaaa gaatattaga
    41581 aaatatttgt ataaaaatat atgttttaag tatatcttta cattagattt tataaattta
    41641 ttaataagaa taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct
    41701 cataaaatga aagtatatca attgataata ctttaaataa gaagggagaa aaaatggaag
    41761 ataaatttta tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt
    41821 cttttaataa cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg
    41881 aaaaatacta tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta
    41941 gcgatattac agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta
    42001 aagagtatcc tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag
    42061 caatgagttg tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta
    42121 agtgtgaagc aggaattaga atatacagtt taaaagaaga ccctttaaat cctaagtttt
    42181 taggatattg ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg
    42241 gtggaagata tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg
    42301 tcatagatat aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc
    42361 aatatgcaga tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat
    42421 ttatggataa aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg
    42481 gttatggagg agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct
    42541 taggtgaatt gccatttacg cctgcatttt ctagtagact tgcaggtgca agaactcata
    42601 cagcattacc attgccagga agagatttag tcgttgttca aaatgaggga gaaagattcc
    42661 agttctttaa accagataac attacagatg ttcaagctat gaataatata catatggttg
    42721 atgttagtga cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa
    42781 aagatttccc ttatcctaac tttaatgttg cgggattagg aaaaccaggg ccatttggtc
    42841 cacataatct tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag
    42901 tatattgctg ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata
    42961 tcaaagagct agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt
    43021 tcccaggatt cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag
    43081 gctacatcat catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt
    43141 aaataaatac taaagaggga gagttgaaaa tgaaaatatt aggtatttct tttggaacaa
    43201 aaaatggaaa taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg
    43261 ctgaagtaga atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg
    43321 cgtgtgttaa aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg
    43381 aatggttgct tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg
    43441 aagaaggggc atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata
    43501 gaggaaataa cattatagga acaaaagttg cagaagctat aggaggaaaa attccagatt
    43561 caagaatgct taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta
    43621 caagagttca atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata
    43681 atgcttggtt cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc
    43741 atcaaattgg tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag
    43801 gagaagaagg tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta
    43861 aaaaggcaat ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa
    43921 ctatatttag ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt
    43981 ttatacatgg tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac
    44041 aagaatttaa agaaagaaaa acatcttata attttataga gacattaact aaagaaaaac
    44101 aagctggagt ttcattagga taataattaa aatactaaga ctctttttta agggatgtta
    44161 gatgttatag gaggcttatt gtatgaatga aaagtcaagt tttaaacaaa ttgaagttat
    44221 tttagcagtt atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat
    44281 tgttatattt ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat
    44341 agtaattaag ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa
    44401 tggatagaaa gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt
    44461 tatgggcagt attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg
    44521 caggattata tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt
    44581 tttggtgtgg agatttatgg gcagtgttag tcattttaat tatgggatat atgaatttca
    44641 atcaaaatct agaattattt ttaacactta gtatattagg tttttttgca gttgtaattg
    44701 catccttatt tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc
    44761 taactattat gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg
    44821 tagctatggc agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata
    44881 tttttagtaa agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg
    44941 agaatggcta tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc
    45001 aaggaagaat atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt
    45061 attagcactt ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca
    45121 aatgaaggtg agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta
    45181 gatgaacata tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt
    45241 ctacgttggc aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgcagaa
    45301 agtggagatg caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc
    45361 tttgttccaa taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga
    45421 ccaaactatc gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga
    45481 gaaaaatggt tctatgatga ggtagtgcca tactttacaa actgttgcta tgttaatcgt
    45541 tttgtcagta gtaaaataat gattaattat ggagcaactg cttttgaccg tgtgtcagaa
    45601 ctatggtttg aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaaatcgttt
    45661 attaaaaaac cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat
    45721 atcgcatcag tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga
    45781 tatataccaa tgagataaaa attatagttt gtgataagta gaggaggcat ttactcttac
    45841 tggtagaaga agacttatta tccttaaaag taggtgaata tatagtttta tatttaaatt
    45901 tatataaaac taagactaat aaatatgaaa caatagaatt agaataattt gataatattt
    45961 gttaaaagag catttagaat gtgtttaaag attaagatat tccttttatg gtagcagata
    46021 gttgaaataa taaaactgtt atcatgaaag gaatatcttt atgtatagga aatttttaaa
    46081 aataaaagtt aaatatgttt aagaacatct taaagtgaaa gaatcattgt tagaaatatg
    46141 tagacaatat agtatttata acgtatcata ttaacaagtt tacacttgga ttatgaaata
    46201 aaaagagtaa ttccaaaggt tgtaaatact atgctgactt tgatacttca aagtttaggt
    46261 gattttgtaa ttgttaatag aaatacgttt aatgagcata aagaatttat ctaataatag
    46321 gagaatataa tgtatacgat tggacaagta tctaaatttt taggaatatc tagagataca
    46381 ctaaaatttt atgaggacaa aggattagta aatcctaaga aaaatgatga aaatggatat
    46441 agaatatata atcaggttga tatctatgat attgcaacaa taaactttta tagagaaatt
    46501 gatatagaaa ttaaaaagat tcaagaaata agaaaaagta agagtataaa caatttagaa
    46561 ctcttactag aagagaagga acaaatcata ctaaaagaga tagaatataa aaagcttctc
    46621 ttaaaaaagg ttcagttagt caaagaagat tgtgacaaaa tcaaaacata ttttggaaaa
    46681 tacacaataa aagaaatgaa gcctttggaa gtaaaaggta aaataaaaca tgttactaac
    46741 tatgaggaat atgaagccct aaaagagaat acaaacaact taaaaaaagc agttacatta
    46801 actgatttaa gaagagtaat aaattttaat gaagaaggta taatagaaga aaaatttata
    46861 attgtaaggg aagtagatga ctacaacaaa atcatagaag gtgaagttct atctcatccc
    46921 aattgtattt atactattat tgaagatggc agatggtcaa ctggaggaga aaatattgat
    46981 aataaagtgg aagacagatt aagagaaata gcaatagaaa atgaatgtga attacttgga
    47041 atagtctatg ttaatatatt gcttactact tatgaagaag ggattgagcg tatattttta
    47101 gagatttatg cacctataaa ataatataaa agcattgacc tgggacttac tcataggttt
    47161 aaactcatga tataagcagt tataacttat atagcaatta aatattaatt tgaaatgagg
    47221 attacttatg agaacagaga aatcattaaa aaggcctata gccagtttta ttattctaac
    47281 aaatataatt tttttaccac tttttttatt ggtagtagtg actataatgt taaaacttcc
    47341 aacagtgata tctgacattg cgttgtgtat atcagcgtgg tcatccactt ttgcatttat
    47401 gatattattt aaaaaaattt atccaggtaa gaaattttta gtacacgtaa aagatagatt
    47461 tagaaataaa cttaaatttt caacagtatc tataataatt agtatacaag tcctaatagc
    47521 tgtagtagtt atatttatat tagcatctaa aaatcatgga ataatgccta actttacagt
    47581 ttcatcatgg ggagcattta tatatttatt tttaaaaaat ttatttgcag gatcattagg
    47641 tgaggaatta ggttggagag gatttgttca aaatcatctt cagaaaagac attctccttt
    47701 aaaagcatcc ataatagtag gtttttggtg ggggatgtgg catttaccaa tatggtttac
    47761 aacaggattc aaagggcttg atttaataaa atatattata ttttttatga ttgcaattat
    47821 atcaatcagt attataatga caacttttta taatctaaat aaaactttga taatacctat
    47881 tgtaatacat caactattta attttttaat agggataatt aatggagatt taatagagct
    47941 tataaattac tatgcaatgt tatatttaat atttgcaatt gcattaatag ttataaatcc
    48001 aggaaatgta ttatataaaa atattagaga gtaatatata tctttatggt aagatataaa
    48061 gaaattatta gaagaacatc caaggaggaa taaacatggt tttactgatt gttgatgcac
    48121 aaaagttaat aacaaacgag aggttatata aatttaatga atttgtagta aatgtagaaa
    48181 atttaattga tacagctaga aaaaataata tagaagtaat atatgtacgt catgatgatg
    48241 gagttgaaaa tgaattgact aaaggtaaaa atggctttga aatatacgaa aaatttaaac
    48301 catgtaatgg agaaaaaata tttgataaaa aagtaaatag tgcttttaaa gaaactggtc
    48361 tattagaata cctaacaaat aatggtgaaa aagatattat tattgctggt ctccaaacag
    48421 attattgtat tgatgccact ataaaatgtg gatttgaaca tggatttaat ataatagttc
    48481 cagcttattc aaatacaact gttgacaatt tttttatgtc agcagaacaa tcatataaat
    48541 actacaatga atttatatgg aatgaaagat atgcgaagtg tatttcatta gaggaaacac
    48601 ttagaaaaat gaaataaaaa tagagtatga attgtatatg tacataagtt gatttcataa
    48661 aaaaggatgt taaattccaa gttaaaaatt agattaaata tattatatta tagataaggc
    48721 acatcaattt tattttaaat taatgtgctt tttaattgta gtaatttatt tataatcaat
    48781 atgattttta ttatttaaat caatttttct tgagtttctt tgtctaaaga aacttttttt
    48841 atttataaaa tctacagtta tattatagta tctattttag agaagtttaa tttttgtatc
    48901 aatctaaaac aaaagttata cttaaatgaa attgaaaacg ataaaattct caaataaaat
    48961 aagtattgtg gaggtgatga aagaatccaa tgataatatg aagactaatc ttaatgagaa
    49021 agtaggggtt aatggtagtg gacaaagaaa ttgtatcaat tctccagatt ttattaaata
    49081 gcacattgat tacaactaat ggattacaag aggagtctaa ttcatcaaag agacaaatta
    49141 cttatagaat aaataaaatc aatgacatgc ttaaagtaaa aaatgtacca cttatatgtt
    49201 taagagctga taaagatatt atcgttagaa aagaaacaaa aaaagcaatt aaagaaattt
    49261 tagaaaaaaa ctattctaag aatacttatt attttagtaa gacagaaaga cttttatata
    49321 tgtatttaat gctttttatt aacatggatg atatttcaat taatcatttt ataaattcta
    49381 taaaagtaag taggtctact gtaaatcttg atttcaagga tttgatacca gagcttgaaa
    49441 agaagaacat taaagttaag aacaatcgta taaatggata ttacatagtt ggcaatgaga
    49501 tggaaattag gagagtgctt attaaaaaca ttatagaaac tttgtctaat gagaaaagtt
    49561 caagagtatt tgatattttt attaaagaat ataatttaga tagttttgaa gaggctaaaa
    49621 aaattatatt aaagctaata aaaaaatatg aaattacttt tgttgaagat agattaatag
    49681 aatttatata tatatttatc tttctaaagg caaggatgta ttcaactaga atagttactc
    49741 aggaaaatgt agatattcct aatatttcag ttatgagttc aatgaaggag tataaatttg
    49801 ctaaggaatt attagaagta tatgggagtg aagataaaat aaagcctcac aatataatgt
    49861 atattagtgc ttggatttta ggtatatcag taggaaatta tgaagaagat acagaagata
    49921 aggatgtaat ttcaaaaatt gtaaataaaa tgatgacaaa gtttggatat ttaagcggag
    49981 tttactatat aagccaagaa aaaatattta aacaattata ttctcatttt agacctgcct
    50041 actatagact tttatttaaa ttacctattt ataatccgat ttgtgaaaaa gtaaaagaag
    50101 aatataaatt agtttataga attgtaagta atgcaatgaa agagttttgc ggattatttg
    50161 gagaagaaat tcaggaagaa gagttagcat atcttacaat gcactttgca acactttttt
    50221 caaataaaaa agaatttgat ggttttagaa aaagaatagc attaatagtt tgttcaaatg
    50281 gcgttggtag ttcagctatt ttacatgcag aacttacaaa cttatttcct gacttacact
    50341 ttataccaat gtcggaatca gttggattag aaaatataag tgaacctgta gatattgtat
    50401 ttactactaa ttataacgca gagggtttaa aaacagatgt accagtaatt aaagtaagcc
    50461 ctgttatgac cacaaaagaa aaatataaga ttacacgtga agtgtatatt caattaggtg
    50521 atattttttt aagacaacct aaaatagatg aggttatgga tataataaaa aaatatgcaa
    50581 atgtaacctc tgaaagtatt ctttcaagtg agctattagc ttattttaca cagatagaga
    50641 attttacatc taaggaagga gaggggccta tgctaagtga gattactaat gaaacactag
    50701 tgaaaataaa ggtaaaagct aagaattggg aggaagctat tagacagtca gcttctgtat
    50761 tagttgaaaa taacaaagta actgaagggt atgtagatgc tatgattaac tcagcaaaag
    50821 catctggacc atacatagta attacaaaac atgttgcact tccacatgct agaccagaag
    50881 ctgggtcaaa agagattgca ataggcattg ctactttaga tagtcctatt gagtttggga
    50941 ataacgaaaa cgaccctgta aaatatgtat tttgtctaag tgcaatagac aataatagcc
    51001 atttacgtgc tatgtctgag ctagttgaac tattggaaga agatgagttt tttaaggttt
    51061 tagatagtgc taaagaagct aaagaaatta ttgattttat aaaaacacac gaattataaa
    51121 ggaggattta attatgtata aaattttaat cgcatgtaga gcaggagttg gctcaagttt
    51181 aatgttaaaa ataaagactc aacaaattat taaggaaaat aactttccaa ttgaagtaga
    51241 gcatggttca ttggattcac taaatggatt caatggagat gctgtagaaa cattaattga
    51301 tgttgctgaa gaattaaaaa ataaaaattt gaagtttgat ataattggga ttcacaatat
    51361 tgttgaccgt aatgaaataa agacagcact agaaaaattc cttgcttcaa aagagggata
    51421 aatttaagat gataaggagg aatatttatg gagtttatta ttagtttgct gagtacacct
    51481 gctgttttgc ttggcgtagt tgcatgtata ggattgattc ttcaaaagaa atcagctgtt
    51541 gaagtattta caggctcttc aaaaacactt atcggttttt tgatatttgg tattggtgcc
    51601 tctgctatga caggagcact tcaaaatttc aataaactgt tccaacatgg ctttggaatt
    51661 actggtgtta tagcttcacc agaagcagct acagcacttg cacaaggtac atatggtttt
    51721 gcagtttcat gtacattaat tcttggattc atattaaatt tagtatttgc acgtattaca
    51781 aagtttaaga atattttctt tacaacaggt catagtttgt tcttttcttg tgtattagtt
    51841 ttaattatga aagcacatgg attcgataat acaatcacta ttttaatagg tggaactatc
    51901 ttagggttta tgtctgctgc actacctcaa ttctgtcaac catttatgcg tgaattaaca
    51961 ggtggcgacg aacaagctat tggtcacttt aacatgattg gttatggttt atcaggttat
    52021 attgggcgtt tattttcaaa acataaagat gatacaacag agactataga atttcctaag
    52081 tggttatcaa tattcagaga cttcttaatg ggtctatcca tagttatgtt aatcttattt
    52141 tatattgcaa cattaaaagc tggtcaagca tttactcaag aaattgctgg ttcaacacat
    52201 tggttggttt ttccgcttat agaggcattt acatttgtgg caggtatgtc aatattgatg
    52261 tcaggtgttc gtatgttctt agctgaaata actgcagcat ttgtaatcat ttcagaaaag
    52321 tatattccag gttcacgtcc tgcgctagat gttccaaccg tatttccata tgcaccaaat
    52381 gcagttatta ttgggtttat atcagcatat gctgctggtc tattagcaat tgctattatg
    52441 gcatcatttc ctagtatatt tcctgtagta attattccag ctgctcacat atgttttttc
    52501 tcaggaggta cagctgcaat atttggcaat acttcaggag gatggagagg ggctattgct
    52561 gggtcatttg ttgtggggtt attacttgca tttttgccag ttatactata tccagtttat
    52621 gggactcttg gtatagaagg agcaactttc cctaatattg attacaacgt agttggttcg
    52681 atattacata atattttaca atttataaaa cctatttaga ctaaattata taaaagtata
    52741 gtcatagtta ctatgatttc aaggaggata catcttatgg aaaatacatt atctgaattt
    52801 ttagataata tatctaatga attgtcagaa tttattgcct caatagatga gacatctata
    52861 aataaagcat gcgaattaat tcttgaagct gaaaaaaatc acggacgtgt acatgtgact
    52921 ggtattggca aaccaggaca tgtatcggga tatatttcat cattgctttc atctactgga
    52981 acgtcagcat atatattgca tggtacagaa gcagtacatg ggtcaagtgg tcaagtagtt
    53041 gaaggagatg ttgttattgc aatatcaaat agtggtgaaa ctcaagagtt aaaagcaact
    53101 cttaagactc taaaagtaaa tggagcaaag ataattggtg taagtggtaa tgagagttca
    53161 tttttaaaaa atatttctga tatattcctg tttgcaggtg ttaaacagga aggagattgt
    53221 cttaataaag ctcctcgtgc ttcaatttta gctgaaacaa taatattaca atcattaagt
    53281 gttgtattac aatatgctaa aggtcttaat acacagcagt atttgaagtg gcatccagca
    53341 ggaagtcttg gaaagtctat tagagaggga atataatttt atgaaatata aagggattat
    53401 atgtgcaatg ataacaccat ttgatgaaaa tcaaaatatt aattcacaag caacatgtca
    53461 attaattgat tatttgattg aaaagggaat ttatggatta tttattttgg gcactaatgg
    53521 tgaatgtcat gtattaacag atgatgaaaa agttgaattt gcaaagattg ttattaatca
    53581 tacaaataat cgtgtacctg tttttgttgg aacagggggt aattcaactc gtgaagttgt
    53641 agatttatct aagagaatgg aagcgattgg agcaagtgca ttatctatta ttacaccata
    53701 ctttgttaca ccaacacaac aggaattaat cttacattac aaaactattg ctaattcagt
    53761 taatttacct attataatgt ataatatgcc aggtaagact ggaattaata ttgaaccaga
    53821 gtcagtacgt gaattatcaa agataaagaa tattgttgga attaaggata gtagtggtaa
    53881 acttgacaac atgaaagctt atattgaagc aacgaagaat gagaatttta gtgtgttttc
    53941 aggctcagat tcacttattc tggatagtct aaaagctggt agtcaaggag ctgttgcagc
    54001 aacaacaaac ttcctcacag agattggtat ctctatctat gaaaacttta tgaaagggga
    54061 tttggaagaa gctcaaaaag ctcaaagtag tatagaagaa cttcgacgta tactgaaact
    54121 tggaacaatt ccatctgtta tgaagaaaac tattgtcttg aatggaatta atgttggaac
    54181 tgcacgactt cctgtaactg aaccaactgg ggaagtactt gaagaaatta aacgagtagt
    54241 tgaaaattat aggacaatac ttaataaaag tagtgagaat agataatata aatagggaaa
    54301 ttacactggt atataattaa tatatatatt ataaaacatg actaaaatta atgaataagt
    54361 aaagtggttt gatgagtata gaggtgataa tatgaaaaag atgaatataa ccaaaagaat
    54421 gtcagaatgt ggggctcttg caattgtaag agaagaaaat cttaaccgtg catgtgaaat
    54481 tgctgaagga tgtataaaag gtggtataac agttatagaa atgagttata ccctaaataa
    54541 tgctggagaa ataattcaag ggcttaataa aaagtatggt gaaacacttt gtgtgggagc
    54601 aggaacagtt ttggatagtg aaacagctcg tcatgctata ttacatggag ctcaatttat
    54661 tattgcacca aattacaatg aaggtgtagc gaagatttgc aatagatatc agattcctta
    54721 tgctccagga tgtacatcat tgactgaggc agtagacgca ctaagtttag gagcaacttt
    54781 tataaaagct ttcccaatat cagatttcta tggacctaaa ctagtaaagg tatttaagac
    54841 accaatacct gatatgccag ttttagcaag tggagggatt aatattgaaa acctacatat
    54901 atggttagaa aatggagttg atgtatgtgg atttggtggt ttattaacta aaggttctgt
    54961 tgaagatatt gctaaaaatg ctaaaaagat tcgtgaaatc attaaaaata taagaaaaat
    55021 ttaacatagg gctgtatcat agtatcttat aattactatg atacagcctt tttaatatta
    55081 ttcaaattta aaacagttta catttctatt atattacata taatttcagt aattttatct
    55141 taatttttct atctggaaaa aaatgattta aaacttaagt tatgatataa tttcattaaa
    55201 agaaggcgat ttttagtaaa tatgagaaaa taatgtgttt ttaggggaaa agttaaatac
    55261 agatttaagt gaacaagaat cgataattaa gatattaaat ggtgaatagg gcatcaaaaa
    55321 aattactaaa ttaaaagttt tagagggaga atcttagcaa gtgtgtgtga aaattcaatt
    55381 tcaaacaaaa aataattgta aatgaaatca atttatacac gttgaagatt cacttttatc
    55441 attatattaa aaagcagagt gaattaaaag tgagtctaaa gataggagga taattaaaac
    55501 aatgaaagaa gacattaggt tacatcgaat gtgtaaaaaa gaacaagcaa caaagaatga
    55561 agttatatca gaggatattc taaaatctat gagtattggg gtcagtaaac attttttaga
    55621 tgattcattt acaataatat ggggaaatac atcattctat caattattag gatatacaga
    55681 agaagaattt ttagttaaat tttctagttt aaaagattat tatgaacaga atctttatca
    55741 ttttgaatct atgaaaaatc attttttaga ggcttatcat catggcgaaa agagtataga
    55801 atatcaaatt tgcatttcag caaataatgg agaccctgtt tgggtaataa tgactggtat
    55861 attaattgaa tcacaagata atgagaaatc aatggtctat atattgtatt caaaaattaa
    55921 tgagttaaaa ataaagcaag aagaactatc tgatatggaa aaagagagta cagagaattt
    55981 taaatggatg atgtcagagt atgctgggaa tgtttatatt agcgatatgg atacatatga
    56041 attactatac ttaaataagc actcatgcaa tactttacag gcatcagcaa atgaacttat
    56101 tggaagaaaa tgttatgagg taattcaagg tagaacttcg ccatgtccgt tttgtacaaa
    56161 ttcgtattta aaaaaagatg acatatatga atgggaattt tataataaaa aactgaaacg
    56221 aacatttata attaaagacc gtatgttgaa ttggcaaggg cgaagagttc gtattgaatt
    56281 atcttatgat atgtatagtg ctgaatataa attagcaaaa aaagatcagg aaagagaatc
    56341 aattttgaaa actattccag caggaatggt tagattagat gcaagagatt atcttactat
    56401 actttggtgt aatgatatat ttttgaatat gattgaatat acaaaggaac agtttgcaga
    56461 ggaacttaat aatcaatgtg gatatttatt ttctgaagat tataaacgta gtaagaaact
    56521 ggcacaagat ttgaaggagt ctggggataa tgttgtattt gaagcaaaaa tatatacacg
    56581 ttcaagggaa gaacgtatat ggactgttac attgtgttat atcagtgggg aagacagttg
    56641 ggatggaata ccatcttttt atagtatagg tattgacata acaaaggaaa gaaaacagat
    56701 tgagaaatta cagcatatat cagagaaaga tgcattaaca ggaatttata atcgtgctga
    56761 gactgaaagg cagattaaga aatattttga aaaaaattta aatgttatgg gtgcactgtt
    56821 tatgattgat acagataatt ttaaacagat taatgataca gaagggcata tgattggaga
    56881 tatagtactt actgagatgg catcaggtat gaaaaaaata atgcgtgaca gtgatgttgt
    56941 tggaagaata ggtggagatg agtttacaat ttttatgaag aatatttcct cagttaaaga
    57001 tgcagaaaag aaagcagaag aactattgta tatgttccgt catctatttc agaaagaaaa
    57061 aagttttcta aaagtaactt gtagcatagg aatagctata tatccaaagg atggtactac
    57121 attcaaggaa atatatgcaa gagctgataa agccctgtat caagcaaaaa atatgggaaa
    57181 aaataattat gtaatctaca accaagatta ttttaaagaa ttagaagaat ttgattgttc
    57241 ttctcttggt acagttattg attcagaaaa acaatacgct gaatatcctg ataatcttac
    57301 acgttatata ttccgtatgc tttatcagac tgatgatata gaccaggcga ttaatatgat
    57361 acttgaggtt gttgggaaac agtttgatgt aagtcgtgtt tatatttttg aaaatacaga
    57421 agatggtaga tatacttcta atacttatga atggtgtaat gaaggaatct cttctcaaat
    57481 ggcttatttg caaaacagca attatcaaga ctatgaggat tatgaaaagc tttttgagga
    57541 tgaattagtt ttttattgtc gagatatcca tacattaagt cctaaactag aagaattgtt
    57601 ttctagtcaa ggtatccact ctacacttca atgtgcatat aaagaagata gagttttcag
    57661 tggatttgtt ggatttgatg aatgcacagg actacgtcta tggtcacaag aggaaataag
    57721 tacattatca ttgatttctc aaattatatc aatgttttta caacgtaaaa aaaataataa
    57781 acttaatcaa cagattcaac aataccagaa tattttagat aatattgacc agtgtatttg
    57841 tgtgattaat aaagaaaata attttttatt atatgtaaat aaaaaattta aaaatacata
    57901 ttctaaattc caaattggac aaccttatta tcttgattct atagatattc ataagatatc
    57961 cattatatgg aataaaaaag aagcatacct atgtactagc acgatttaat ggtaaagtaa
    58021 agtttatatt ggacttaaat taaatttcgt tttaaataag atattatttt agttgcttac
    58081 tgaaaagact agaatctata ttaatgaatt atactaattt tataccacta ttaaaatatt
    58141 gtttgctaaa aataaaaaat agccttaaaa cagttgatta taaaaaatta actgctttaa
    58201 ggctattttt atctttgtga cgaaatagac acttgccatt tagaatgtga caatatagta
    58261 gttgtttaga aaaggcacaa agcattatac tttaactaaa gtaaaatgta ttttgctttt
    58321 agaataaaaa caattaggag ggagctataa ttgaaaaaga taatatatgt attatttaca
    58381 attgaactat taatagtatc tttattaggt ctaaatatag ttaaagataa tgaaattaat
    58441 aatattttat ataacgatac aacaagtata tctgtaatgt ttaaagatta taaaaagtta
    58501 aataaaaatt atagtaagtg gattaaagat attgcagatg aaaataatgt ggctatatca
    58561 aagtatgttt ttaataataa tgaaaaatta tctatatata cgactgatac aagtttgaat
    58621 aacaatataa agttaaaatc aggaaaattt cctaatacta attcaaatga atttattagt
    58681 aatataaaca ctaataatag taatcaagta ggtaaattct caactatgag taaagacata
    58741 tcaattttaa ttagggattt tggtaaaata agtgaagttg gagaatcagg aatcttatat
    58801 atttcaactc aaaatgaaga tacaataaat aaaatattaa gagaactaaa tgtagacaga
    58861 agtatttttg tagcaagatt acatgataga tatgttaact caaatttata cttaaatcct
    58921 tcaataatta gagatttaat actaattatc ctttgtttct tggctacaat aatacattat
    58981 tcaattagtg tgagtagaga ggcttctatt ttaagattaa atggacactc taagctagat
    59041 ataattgcta gagttataaa aaatatactt agaattatga ttttatcatc tgtcacagca
    59101 ttattaatat atataatcta tattttaaaa ttaaatatag gtgttag
//
LOCUS       DAELJO010000007        50971 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELJO010000007 DAELJO010000000
VERSION     DAELJO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.007"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(104..784)
                     /locus_tag="KPB88_000364"
     CDS             complement(104..784)
                     /locus_tag="KPB88_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0052674.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(936..2843)
                     /locus_tag="KPB88_000365"
     CDS             complement(936..2843)
                     /locus_tag="KPB88_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0052675.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2844..3596)
                     /locus_tag="KPB88_000366"
     CDS             complement(2844..3596)
                     /locus_tag="KPB88_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052676.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3912..5066)
                     /locus_tag="KPB88_000367"
     CDS             complement(3912..5066)
                     /locus_tag="KPB88_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0052677.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(5069..5536)
                     /locus_tag="KPB88_000368"
     CDS             complement(5069..5536)
                     /locus_tag="KPB88_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0052678.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5550..6038)
                     /locus_tag="KPB88_000369"
     CDS             complement(5550..6038)
                     /locus_tag="KPB88_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052679.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6052..7383)
                     /locus_tag="KPB88_000370"
     CDS             complement(6052..7383)
                     /locus_tag="KPB88_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0052680.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     DRQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7397..8962)
                     /locus_tag="KPB88_000371"
     CDS             complement(7397..8962)
                     /locus_tag="KPB88_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0052681.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9131..9916)
                     /locus_tag="KPB88_000372"
     CDS             complement(9131..9916)
                     /locus_tag="KPB88_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0052682.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10083..11282)
                     /locus_tag="KPB88_000373"
     CDS             complement(10083..11282)
                     /locus_tag="KPB88_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG0052683.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVNDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDDEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(11804..13141)
                     /gene="xylA"
                     /locus_tag="KPB88_000374"
     CDS             complement(11804..13141)
                     /gene="xylA"
                     /locus_tag="KPB88_000374"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG0052684.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13237..14787)
                     /gene="xylB"
                     /locus_tag="KPB88_000375"
     CDS             complement(13237..14787)
                     /gene="xylB"
                     /locus_tag="KPB88_000375"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG0052685.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKIND"
     gene            14995..16155
                     /locus_tag="KPB88_000376"
     CDS             14995..16155
                     /locus_tag="KPB88_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0052686.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16311..16715)
                     /locus_tag="KPB88_000377"
     CDS             complement(16311..16715)
                     /locus_tag="KPB88_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0052687.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16736..17218)
                     /locus_tag="KPB88_000378"
     CDS             complement(16736..17218)
                     /locus_tag="KPB88_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052688.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17238..18047)
                     /locus_tag="KPB88_000379"
     CDS             complement(17238..18047)
                     /locus_tag="KPB88_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0052689.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18040..18819)
                     /locus_tag="KPB88_000380"
     CDS             complement(18040..18819)
                     /locus_tag="KPB88_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0052690.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18819..20897)
                     /locus_tag="KPB88_000381"
     CDS             complement(18819..20897)
                     /locus_tag="KPB88_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG0052691.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(21693..22214)
                     /locus_tag="KPB88_000382"
     CDS             complement(21693..22214)
                     /locus_tag="KPB88_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0052692.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            22380..22946
                     /locus_tag="KPB88_000383"
     CDS             22380..22946
                     /locus_tag="KPB88_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0052693.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(23163..25226)
                     /locus_tag="KPB88_000384"
     CDS             complement(23163..25226)
                     /locus_tag="KPB88_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0052694.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKDTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(25607..28942)
                     /locus_tag="KPB88_000385"
     CDS             complement(25607..28942)
                     /locus_tag="KPB88_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0052695.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYTNDSDLLLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(28992..29420)
                     /locus_tag="KPB88_000386"
     CDS             complement(28992..29420)
                     /locus_tag="KPB88_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052696.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLTCEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(29410..29790)
                     /locus_tag="KPB88_000387"
     CDS             complement(29410..29790)
                     /locus_tag="KPB88_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG0052697.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTKALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(29823..30131)
                     /locus_tag="KPB88_000388"
     CDS             complement(29823..30131)
                     /locus_tag="KPB88_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052698.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(30164..31264)
                     /locus_tag="KPB88_000389"
     CDS             complement(30164..31264)
                     /locus_tag="KPB88_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG0052699.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIL
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITYEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            complement(31268..32731)
                     /locus_tag="KPB88_000390"
     CDS             complement(31268..32731)
                     /locus_tag="KPB88_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG0052700.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDGHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVIYDAPNVILGRVKELEKEIAVGLEELERMLEV"
     gene            complement(33241..34110)
                     /gene="gatY"
                     /locus_tag="KPB88_000391"
     CDS             complement(33241..34110)
                     /gene="gatY"
                     /locus_tag="KPB88_000391"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0052701.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTNPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSIPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(34130..36046)
                     /locus_tag="KPB88_000392"
     CDS             complement(34130..36046)
                     /locus_tag="KPB88_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG0052702.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSETSR
                     EIVSKLNNEEIKSV"
     gene            complement(36049..36969)
                     /locus_tag="KPB88_000393"
     CDS             complement(36049..36969)
                     /locus_tag="KPB88_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG0052703.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(37071..37823)
                     /locus_tag="KPB88_000394"
     CDS             complement(37071..37823)
                     /locus_tag="KPB88_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0052704.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(38215..39660)
                     /locus_tag="KPB88_000395"
     CDS             complement(38215..39660)
                     /locus_tag="KPB88_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0052705.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(39682..40035)
                     /locus_tag="KPB88_000396"
     CDS             complement(39682..40035)
                     /locus_tag="KPB88_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0052706.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(40064..41311)
                     /locus_tag="KPB88_000397"
     CDS             complement(40064..41311)
                     /locus_tag="KPB88_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0052707.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(41319..41636)
                     /locus_tag="KPB88_000398"
     CDS             complement(41319..41636)
                     /locus_tag="KPB88_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052708.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            41878..43791
                     /locus_tag="KPB88_000399"
     CDS             41878..43791
                     /locus_tag="KPB88_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0052709.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDKKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(43914..45068)
                     /locus_tag="KPB88_000400"
     CDS             complement(43914..45068)
                     /locus_tag="KPB88_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG0052710.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYEVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(45347..47962)
                     /gene="mngB"
                     /locus_tag="KPB88_000401"
     CDS             complement(45347..47962)
                     /gene="mngB"
                     /locus_tag="KPB88_000401"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0052711.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDESSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEIGHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(48106..50031)
                     /gene="mngA"
                     /locus_tag="KPB88_000402"
     CDS             complement(48106..50031)
                     /gene="mngA"
                     /locus_tag="KPB88_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG0052712.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(50161..50886)
                     /locus_tag="KPB88_000403"
     CDS             complement(50161..50886)
                     /locus_tag="KPB88_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0052713.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
BASE COUNT        16519 a         9128 c         5308 g        20016 t
ORIGIN      
        1 tatttatttt taaaaatatc tatagcaaat aagtagatat ttattatact aactacaact
       61 aaaggtagag caatatactt agacctacct ttcaaatatt ttactagcat tgtattatat
      121 atcctatccc cttcttagtt tttataaaat catcaaaacc tagttcttta actttacttc
      181 tcaatctatt catatttaca gtaagtgcat tgtctgtcac aaactcatca ttatcccata
      241 gagtcatcat aagactttcc cttgatacaa ccctattctt attttttaat aataatgtta
      301 aaatttttat ttcgttttta gtcaactcta tacttttatc cttatactta tgttttataa
      361 tccctttccc cacatcaaac atcaaatctt cacaatacaa aatactatta gaatcactat
      421 aattatagac tcttcttatt ataccattta ttttggtaac caatatttct atagaaaatg
      481 gtttggtaat gtaatcatct gctccattat ttatacccat tattaaatcc atatcagaat
      541 ctcttgatga taaaaatatt aatggtacat ttgaaatagc tcttattttt tcgcaccagt
      601 aaaatccatt atataatggt aaatttacat ctaaaagtac tacatctggt tttttatcta
      661 taaattcatc taatactacc tcaaaatcat caattaattc tactttaaat ccccattttt
      721 caagtgctaa tgcaatctct tttgataaat caatatcgtc ctctactaat aaaattctat
      781 acatttgtat attctccatt atcctaatat ttcactatgc tatgaattat taatatcata
      841 acatatttta tatgaattta ttacccaaat tatttagtta atcaacaagg agactcttat
      901 tacaagaatc tcctttctct ttattaatat atcaactaat ttttatacac tacactctta
      961 aacttattaa cagatactgc tccatatatt atgaatatta tcaaacatat cactaaactt
     1021 agtatatata taccaaataa atcggtcatc aataaatctc ctgctaatct caatgcaaaa
     1081 taactagata tagttgcact taacattggc aaagtataaa aaatagctac ttctttaagt
     1141 atagctctat ttatatcttt ttctgtagta cctattttta acaaaatctt atatttctct
     1201 ttatcttcat atgcatcact atatattttc atatatatta tacttccagc tacaattgca
     1261 aagactaaga ataaaaacac aagcactaca tatgcaaact tcatccattg actctcacct
     1321 tgagctttat atccattttg aatatacaca ttttttaaac ttaaattctt ttcaatattg
     1381 ttgaacatta cttgactatt ttcttcttga aatacctttg caccataaaa atacacttgt
     1441 tgacccttat ctcttagttt aaaatatgta tcatcattta ccactataat actttgatta
     1501 agtcctgtcc ctagtactgg aacttttact tctccccttg aaatattata taactgacca
     1561 tcaagtttta tcttggcttc ttttgtagct attgatgcta tcgtttgagg gctttttaac
     1621 tctattacat tatttccact aactaatctt tcaccatact cttttaattc tttatcattg
     1681 ccattcactt taagagtatt acaaaaatca ctatatttca ttatagtatc tgttgtatca
     1741 taagcttctt ttatatctgt tttctttaaa attgttagtt ctggatttac tgagtacttt
     1801 atcttattag tatcttttat tatattctct atttgttttt tattcacttc atctttatcc
     1861 attgaaacta tgctaatagt gtacacattt ctttgttctt gggcattctt atgtatacgc
     1921 ttcatcgcaa ctgaagcacc aagagttgat attgttgtag ttataattat agctattata
     1981 gcataagttt tatagttttt atttaatcgg tagtttatat tgtttattgc aattatatta
     2041 tttccattaa ataatatttt tttatttttg attataatat caaatataac aggcataact
     2101 gatttgaata atccataagt acctactatt attataccta taacaggtag tacactagtt
     2161 atatctccag ttcttatatt ggtagaaaga tagtagccat atcctattac tataattgat
     2221 actatagcta atatatatat acctattcct acctttggta ttttttcttg ttttttagat
     2281 atattgatta gatttattac actacttcta catattgtta taaaaccttt tacagccatt
     2341 attataaata tagaaataaa gattaaagta gcatatagta tagcttctat actaagtcca
     2401 ttagatacct ttatattaaa tccagataac tttattatca ctacttgaaa aaatctagat
     2461 gttattatcc ctataattat tcctgttata caagaactta ttcctataag catattttct
     2521 acaaaatata tttttcctat agtatataaa tctaatccca taaaagacaa tattccaatt
     2581 tctttagacc tattcttaaa aaatatattt gttgcatacc atataaagaa aaataaaaat
     2641 actgatagta tcattattac agctactaac actgatagtg taaatttttt attttcatct
     2701 tgtaacacat ttatagcatc accatatctt aataattcaa aattagttag cacaactact
     2761 gaaaaaacca tagatataaa aaatgctaga taagttctta tgttactctt aaaattatta
     2821 taagcaattc caagtatact cacttacatc tcacctccca tagaggctag catatccata
     2881 atttttccat aaaattcttt tctactattt cctttgttta atttacactt tatatttcca
     2941 tcatttatca tatatacttc atttgaataa cttgctgtta gagggtcatg tgtaatcatt
     3001 ataatagtag cgttattttc tctgtttatt ttttctaatg attctaaaag ttcatatgct
     3061 gatttagagt ctaaagcacc agttggctca tcagcaaata tgactgttgg atttgtaatt
     3121 aatgacctag ctgctgcgcc tctttgtttt tgtcctcctg ataactgata tggatattta
     3181 tctaaatgat tttctaatcc aaatttttta gctatagaaa ataccttagc ttctatctcc
     3241 ttacttttct tttttcctaa tgctagagga agtgctatat tgtccattag tttcatattg
     3301 tctaatagat tatagtcttg aaatataaac cctatttttt ctcttcttaa ttttgatagt
     3361 tcattatttt ttacttttga tatattaatt ccatctagta ttatttctcc tgaagtttgt
     3421 ttgtcaagtg tagacattaa atttagcaat gttgtctttc ctgcaccaga tggacccatt
     3481 attgatataa aatcaccttc attgactgta agagatatat tttttaatac atttgtttta
     3541 aatcctttta ttccataatc ttttgatata ttttttattt ttaatatttc tttcataata
     3601 atagcctcca gttgctaatt ttttaatata tatttatttt aaaggtttta tataaataaa
     3661 tccttaagta aatattacat tttaactgtt agcattacac ttttgtaaca tatggaagac
     3721 tattataatt ataatggaaa tatcttaaaa taaaagcatg atttacttct ttgtataaac
     3781 aaagaaaaga agctcctaag aagcctctaa tctatacaat tattcttctc tataaaaaac
     3841 caaaatacac attgattgtg gcaaatacag atactataaa caagtgatat tactactata
     3901 tattaaaaca attattcctg aagataattc aataagacta tcaagcaata agctaagcat
     3961 ccaaaaagac ttaataccat taatgtataa ttatacccaa tattaatgct caaaaaacca
     4021 gcaacagttg gaataatcag agatgaacct cccataaata tacttaaagc tattaattgg
     4081 ctactacttc taagtttttt aggagaaact tcaaatatta atctttttga agtaattaaa
     4141 attaaaggag tagaaaacac ttgaaaaaca cacagtataa taatgatatt tgaatcattt
     4201 gttaaagcaa ataatagaaa ctgaactgtt atcataatag cacttaattt taataagttt
     4261 aaactgctat atttttttaa taaataagaa ccagccagat atgttggtat ctctaacaat
     4321 gactgtaaag accactttaa agatatctga gaatttgtag catttaaagc tatcatttta
     4381 tctattacag tacagttatt tgctaccacc attgagtata ataagaataa aatacatact
     4441 agtaagatat attttttatc ttttaataat tcaataatat ctttattagt tacatttatt
     4501 ttcttattaa tcttttcaat atcaggtaat aatagtatat ttataataag taatactaca
     4561 attaaaccaa tcgctacaga taatccaaaa tatgagaatc taagaatcaa gtaagatgct
     4621 accatacttc caatagccca tccaatcgac ccaaaagtct taataaatga caaataatta
     4681 ttaatattat ctccacaacc tattatccat gtatcatata aactacagca agtatttaat
     4741 aatcctcctg ataaagcaac aagtaataag tatattataa aagttgcaga ttcagtagaa
     4801 aacataataa ctgatgcaat tccataaact gataaactaa ttaaaacaat ttttttcatt
     4861 gtttgatact tatcagctaa tactccaaat aataattgaa acaatatatt ggttattgca
     4921 taactcgata atataattcc tctttcaaac gctgagtaac ccaattttga taagaaaggt
     4981 gtgaattttg ttgaagcaaa ggcaaaaata atatatgaga aaaaatagaa tatgataaat
     5041 ttaaatttaa tcgctttatt aatcataatc aatcaaaatt gataacttta ataccactat
     5101 ttccttcctg atttaagttt gaaagatgat attttaccgt attaggattc tctacaataa
     5161 ctaaaggaat atataaagta ttttcaagta acttattatc aatttctact aacttttccc
     5221 ccatattaac ttcttgacct tgtttaactt ctaatttaaa gttttctttt tttgttgttg
     5281 cactatccaa tccaatatga ataagaattt gaacatcatt ttgcaattta attcctatag
     5341 catggttagt ttgagcaata aaacttatta ctcctttagt cggtgattta agaatattgt
     5401 cctttgctat catagcaaat ccatctccca ttaccttagt tgaaaatact ttatcaggaa
     5461 tttctccaag attaattatt tttccatcaa tgtatgaatt aattgtaatt gcttcttttt
     5521 ttcttttaaa gaacattata gacactcctc tacataatta actttattct aattatcatt
     5581 acaaataacg atattaagca tactgcaatt gaggaaaaaa gagccttata gctctttccc
     5641 cattgtagtt ctttaatact gcttatgatt tgtttaattc caacaaattt ataaatatag
     5701 gtaaacatga taacaatagt tattgtgtaa atttggaata tctgccctaa taagactaaa
     5761 tagaaagcaa tcatgttttc ttttatctct ttattctttt ttatattgta aataacatat
     5821 gcactgtata ctgcactaaa gttagagatt aaatttaaac ttgcattaac atcattgttt
     5881 aaaagttgtg aaaatgaaat atttgtaaat aatgatgtca attctaaata cactatatat
     5941 aaaacaggta atattagaaa acaaaccata accactgaaa gaaatatatt ttcatctttt
     6001 aatactgctg tgatattttt tattttattt gttatcatgt ttttgttctc cttatttaaa
     6061 agttggccag aaatccttat tagccacaat catttcatct aagatttctt ttgctacttt
     6121 tgcacttgga acagttttac tcatagtaaa cgactgccat aatttttgat aacttttttc
     6181 tatccatgct tctacagcta atttttcaga agcaacttgt tgttccatta aacctttttg
     6241 gaaagttgga attttaccta tagacatagg ttcaaccata tcttttccaa caaggcaagg
     6301 aatttcaacc attgcatcat ctgcaaaatt ttcaatagct ccattattag gaacaatcag
     6361 taaaaatctt tcctgagtat taaatgctaa tgcacaagct aattcaacaa taaattgaga
     6421 atgctcactt gcatcaaacc atgtatccct tgctgtgcca ttttttataa ttctttcaca
     6481 ttcttcgaat gtatcctttt ctcttctatc cataatttca tttgctcttg tataatttgg
     6541 attagcatgt tcaacagttt catcagccat taaataatat tttaaataag tattaggtaa
     6601 tgtagttggt tctatttcca ataaatcctt tgctgctctc atagtttcta accaactaga
     6661 ttctttgtgt tgaccttgag gtgtaaattc ctcatatcct tttttagcac aatattcctg
     6721 taatttaggc attaaatcat ttccttcttt gtccttaata cttgtccacc acccgaaatg
     6781 attcaatcca taatatctaa catcaaagtc tttacgagaa tttaaaccta aaactctagc
     6841 aaatatttcc tctaaacata ctggcatatc acaaatatta ataattcttg aatttggtct
     6901 taatacacga catgcttctg caactatagc tgctggattt gaataattaa gcatccaagc
     6961 atttggagaa tatttttcca tataatcaat catttcgatt attggaccta ttgttcttaa
     7021 tccataggca attccacctg ccccacaagt ttcttgacca acaaccccat attttagtgg
     7081 aattttttca tcttgttcac gtaaagcata taaaccaaca cgaatttgtg ccattacaaa
     7141 atcaatatca gtaaatgctt cttctggatt tgttgtataa ctaaatttaa tttctggatt
     7201 tttctctcta acaattattg cacatgcttc agcaactttt ctttgtctat catcattatt
     7261 atcatataat ttaattgatt caataggaaa tttagcaaca ctttcaatta acattgttac
     7321 aattccagca gtataagtac tacccccacc agcaattagt atcctctgtc tttttctttc
     7381 catctttcct tctcctttat aataaatttt caaattcttc tcttacactt ggtactgaaa
     7441 gcccaataat aatctgaata gcttttccat tatgcactaa accatgtgca cctactgcct
     7501 taaaatcttc ttctttagaa ataattgagt catctttcaa ggttaatctc aacctcgtag
     7561 cacagtttgt aacatctaca acattttctg ctccaccaac taattttatg tattcagatg
     7621 cttgttgaga ggctttactc aatttatttc ctttttcatc tttagtttct aagtattcat
     7681 ttttagtata tagttttgtt tcttcactat cttcctctct accaggagtt ttcatatcaa
     7741 acttcttaat taagaaagta aatacaaaga accagataaa tgaaaatata atccctacaa
     7801 caacttgaac aacatatacc ttccagtgat acattccaag aggaagccaa tttaatacaa
     7861 cccagttaat aattcctcca ccaaaatcac caacaactcc tagggcaaac gaaatcgttt
     7921 gcaagcatgc atctaaaaat gcatgtacta ggaataaaag tggtgctaca aataaaaatg
     7981 taaattctaa cggctcagta attccagtta atactgcagt aagagtagca ggaatcatta
     8041 aacctaatac ttttttcttt ttttctggct ttgcagttac atagaaagca ccaaatacac
     8101 ctaatgaacc aaacaccttc gataatccgg ataatgaaaa tccgattgga tacatttcct
     8161 ttagagtctt agctgaagtt tgaaagtcct gaatatgagt agcccaatat actgatgtac
     8221 cacctggaac aacaacacta tcataacaaa ttggtgcata tacaaaatga tgtaatccag
     8281 ttggtattaa aatcttttgt aagaatgcat aacaccaaac tcctatattt ccactattta
     8341 taaaaaacgt ttgcaaatgt ctaataacat tttgaatatg aggccaaata aaacaagtta
     8401 ataatgcaac tggtatcata acaaagaaac aaattgcaac tacaagtgat gagcctctaa
     8461 ataatccaat aaaatctggc agttctttat cataaaatct attatgaata taaatgacaa
     8521 tacctgatat taacaaagca ccaaccatac cagtatctaa tgttttaata ccagcaatgc
     8581 ttacaagtcc acttccagca cttatttcag ctgcaaaatc aactccaaag ctttttcccc
     8641 aaaaacttaa catagaacct acaaaataat taaaagtcaa atacgttgct aaagcctcca
     8701 tacaagctct tgcttgttgc ttttttgcaa gtgctattgg taaagaaata gcaaataata
     8761 atggtaattg attaaatacc atccaagctc cactagatat tacaccccaa aattggtacc
     8821 aaatattttc tggtgtagca agtgaaccca taattgactg attttgaaat acaattgaga
     8881 atccaaccat tatcccagca aaagcaaata atagtgttgg tgtaaacatt gcccctccaa
     8941 aacgctgtat tttcttcatc atataaatta ctcccctcct catattttta gtttatcttt
     9001 ggccttagat atctattatt ttaacaggtt aataaatata tacaatcttt agggtacatt
     9061 cggagcttat tcacagtcaa ataagttttt gcacattttt atgttcaaat tctcataaaa
     9121 aaaagaatga ctacaatttg caatcaatcc cttctgtaat cattctatgc cttttataaa
     9181 tactatattt aataaataat aactcaatca atataaaata taacattgtc aatttataac
     9241 tagggtgcat ttgtaaaaat gagatattag tcgctgaaat ataaattgat tcatcactta
     9301 aatcagtcaa tgtattattt ttaaactctg taattgaaat tattttagtt cctattaatt
     9361 ttaatcttct agcaatatta agtgtattct cattctcacc acttagggat ataataatca
     9421 taacatcatt tttactgaca atattgtaaa aagaatcatc aaatgttctt ccctcaaaat
     9481 catatattat ttcttgacat gataagaaca ttctctttat ctgttttgca actacatttt
     9541 gaatatcacc tgaaccataa gcgtatattg ttcttgattc ataaagaagt ttacacaatg
     9601 catcataatc tttatttttt tgatcttcaa catacttaat tattgaatta catgcaatat
     9661 caaatgactg attttctatg acatgaacct tttctgattc ccagcttaat aatactttaa
     9721 actcactgaa accttttaaa tctaattttt taacaaatct tgtaatagtt gcacgagaca
     9781 cattacattt catagcaagt tcatttattg acatcttact acaatgtttt ttatttgcag
     9841 ttatatattt ccatattaat aaatctgtat cattcaattt attataattt aaaaaaatca
     9901 tttcttctaa tctcataaac tattaccccc tttaattaaa aatcgtcata aatattatac
     9961 caatattcaa cattaaatta cagcgtattc ttatatttac tatttagccc ataaaattga
    10021 ctataaaaaa tactgctata attgatgttt aaaaaaatgt agttttaatt ttattctttt
    10081 atctaataat taatattttc aagttctagt gcttttctta tacaattatc atctttaaaa
    10141 tcagcagtcc ttttacaatc ttttacttta aattctggaa tagttttaaa ttcctcatca
    10201 catattccat tcttatttaa atacatacaa cttgccattc taactattag accactattt
    10261 tttaaattaa acaatacagg gtctatacac cctccatctc cgccagtagt ttcaccaact
    10321 aaagtcgcaa atttagtttc tttacaaaac attgcaaatg actctgatga tgaatatatt
    10381 tttctatcaa caagtaagta tatattaccc ttaaattttg aatcatctgt agattttata
    10441 gtataggaag tatcttcaaa atcattaaaa tcttttataa tttctttagg tgcattttcc
    10501 tttacacttg ttggtaaatt ttctacagga tttaatttta tatttcttgc atttgtatat
    10561 tgttttacta tttcattatt atttctataa attctataac catttatttt gacatcattt
    10621 tttatgagtt tagatacgat tccctgccaa taactatcac ttccacctaa attacctctt
    10681 atatcaataa ttaaagcttt atgtttttct aaagtgtcaa tataatctcc aattattttt
    10741 aaatcattat ttatagagcc attctttgat gacattgatg gtaaatacat atatccaatc
    10801 tgacccttaa taacatcctt taaaattaat tcttttttta caaaaatatc atctggcatt
    10861 tttattttaa gattcataga gtcatatcta tctacaactt ttttatcatt aagaaaatca
    10921 taccaatcat tattagagta tgatttctta aaaagttcat atcttttctt attatctatt
    10981 acttctgtgt gtttattatt taaatcagat aatatagatg acagttcttc tataaattcg
    11041 tcatcatttt cagtgttttt tattcgtttt gtatattcat ccttatttgc taaccaatca
    11101 atattattaa gcctcttatt tacacctaga tatggatatc ctgtctttac aacattatac
    11161 acataattaa agtcattcac cttttcctct tcagacaact gattcttttg agaataagta
    11221 gaattaccta ataagattat aaatgctaaa ataattacta aaaataatac tttctttttc
    11281 atgtaattct catccacttt cagattttat attttaatac attgtatcat tattttatta
    11341 tcacctaaac agttaaaaga aattcttgaa gatatattcc actattattc tctttaaaaa
    11401 tttttatttg tattttcaaa attatttcat gaaagttata aaataaaaat tatattcaaa
    11461 tatctatttt actgctatat cctgaaagta aattcttatc agtgatatac taaaaaagat
    11521 atgctaaaaa atttatccat tataatgact taaatttaga tactttactg atttttattt
    11581 attagttgag aaagatattg ataattaaaa atattgataa ctatagaaat gattattttt
    11641 tatagaaatt ttaaggatat aaactaatta tacttaagtc tatatcctta atttatcatg
    11701 attatgagaa tttttacaaa tacttatctt tgtccgtttt agaataaaaa caagaacata
    11761 tcctgttatt ttattcaaac atccaagttt aattactaca caattactta ttagaaattg
    11821 tatctacttc atatatatat ctgttaagta tagcttctag catttcttgt cttccagact
    11881 tattctttat aactggcatt tgcagtgcat aagattctaa ctctttaaat ccaaccttac
    11941 cttctactat atccttacct ataccttcac taaaacttgc atatctttct ttagtaaagt
    12001 tttcaaatac ctcatcttct aataatttat gagctattaa taatcccttt gcaaatgtat
    12061 ccattcctgc aatataagct aggaataagt cttctacctc aaaagatgct cttcttactt
    12121 tagagtcaaa gttaaatcct cctggtgcta ttccaccttg ctttaacact tcatacatag
    12181 caagtgttgc atcatatata tttgttggaa attggtctgt atcccaacct aataataggt
    12241 ctccttgatt tgcatctata ctacctaaaa cattgtttat tcttgctata tttaattcat
    12301 gttggaaagt atgtcctgca agtgttgcat ggtttgcttc tatattcatt ttgaagtatt
    12361 tatccagatt atactttctt aaaaatccta aaacagtagc agtatcaaaa tcgtattgat
    12421 gtttagtagg ctcttttggc ttaggctcta ttaagaattg tccagtgaat cctatttcct
    12481 tagcataatc tacagccatt tgtaagaatc ttgcaaagtt atccatttca agttctgtat
    12541 tagtgttaag taaagtttca taaccttctc ttccacccca gaatacaaaa ttttctcctc
    12601 ctaagtactt agttatttcc atagcttttt taacctgagc tgctgcatat gcatatacat
    12661 ctgcattaca tgttgtagat gcaccatgaa caaatcttgg atttccaaac atattggcag
    12721 ttccccataa taactttata cctgtctttt ccatattaac ttttatatac tcaactattt
    12781 catctaatat cttatttgtc tcttctaaat ctcttccttc tggtgctata tctctgtcat
    12841 gaaaacagaa atactcaatt cccatcttag acataaattc aaatcctgca tcaactttaa
    12901 tctttgctat ctcaattggg tctgttatat tattccaagg tctctctact gtgcctactc
    12961 caaatgggtc attaccctct ccacataaag tatgccaata gctcattgca aatcttaagt
    13021 gttctttcat agtcttatta cctaccactt gttcaggatt gtagtatcta aatgctaatt
    13081 cattgtcaga ttttactcct tcaaatttta tctgtcctat tcctttaaat atttcactca
    13141 tttcatgtcc tcctaatatt ttacacatat aatatatgta gtatgttttc ttaatttaaa
    13201 actattttct atctttattt ttctttttaa gctaatttaa tcatttattt ttgcattaac
    13261 tacttcatct aataacttaa agtcatcttt caaagatttg tatattcttt tatataatct
    13321 atgatatata ttgtatgtat ctacattttc acttattggt tctaatttag ttgaagtttt
    13381 tattatagca tcacatgctt tttgtatatt ttcatatatt ccagctccta ctaatgcaag
    13441 aattgcaact ccaagtgctg gtccttcact tgaatttata gtatatacat cttgattaaa
    13501 catatctgct tgcatctgtc tccatacttt actttttcca cctccacctg aggctcttac
    13561 ttcattaacg tctatattaa gattctctat tatatccata cagtctttaa gactatatga
    13621 aacaccttcc attattgacc ttgcaatttc ttttttagaa tggattggag ataatccaaa
    13681 gaacactcct cttgcataag ggtcaagatg aggagttctt tctcccatca tatatggtaa
    13741 gtaaagcaat ccattacatc ctgttggtac tttacttact tgattattta taatctcata
    13801 tacatcttca cctaaagaat ttgaaacttc cacttcactt tgacagaaat tatctttaaa
    13861 ccattttaat gacaagccag ctccttgagt aactcccata acatgccatg tatttggtat
    13921 tgcatgacag aaagtatgaa ctctaccttc tttatcaatt tttggctctt ttgtataagc
    13981 aaatactaca cctgatgtac ctatagtaga tgaaactact ccatctttaa ctattccatt
    14041 tcctatagct cctgctgctt ggtctccagc tcctcctacc actattgttc cttctttaag
    14101 ccctgtcaaa cttgctacat ctctagtcac ataacctgta atctcttgtg attcataaac
    14161 tttacctaaa agatttttat ctatatttaa cttttctagt acttcatcgc tccaatctct
    14221 ttttcttata tcgagcaact gcataccact tgcatctgat acctctgtag catattcacc
    14281 tgtcaatttg tatcttatat aatcttttgg aagaagtatt ttattagtat ttttatatat
    14341 atctggttca ttgtttctaa cccataatat ctttgatgct gtaaatcctg taagtgctgg
    14401 attagcagtt atttcaatca gtctttcttc acctactaaa tcagttatct cctcacactc
    14461 ttttactgtt cgttggtcac accatattat agagtttctc aatgttttac cttcattgtc
    14521 cagcataact agaccatgca tctgccctga taatccaatt ccttttatac attcactgct
    14581 tatatttgaa ctttgaacta cttctcttat agattcgact acagctttcc accaatcatt
    14641 tgcatcctgc tctgcccaac ctgatttctc ttgtataaga ggatattcat atgtacaact
    14701 ttttatagta tttcctagtt tatcaaataa aactgttttc gtacctgatg taccaatatc
    14761 cacacctaat acatactcca acttcatccc tccttgaatg ccacttcata actatcgttt
    14821 tcctatccta atttttttat attttaggta tattttactt gctttatttt tgttagtata
    14881 ttaaactaac ttatgaacta ataataaaat atttaattat ctttgtcaat gcaaaataac
    14941 cttttatttt tgtgtaaaaa gtattacaat attattttaa ggaggaatat caaattggtt
    15001 actgataaat acactattcg agaaatgaat gaaagattgg tacttgaaca aattataaaa
    15061 aatggtccta tttctagggc aagtatagct tctactatag gattgaataa agcaactata
    15121 tccgctatta ccaaaaaact tattgatgaa tctctagttc atgaaatagg aattggaaat
    15181 agtactcata gtggcggaag aaaacccata ttattagtat ttaataaatg tgcagggatt
    15241 tctttaagta tggacattgg atacgactat atattttctt ctctatctta cttagatgga
    15301 actattataa attctaagaa attaacagat atacaagtaa gtaaagacaa tgttatacaa
    15361 ttgatagatg aaattataaa tagttataac atatccaaaa tagacactcc ttataaggta
    15421 attggtctta ctttggctat acatgggatt acttgtgaaa ataaagtatt gtttactcca
    15481 tattacaatc ttaatgagat tgacttatat agcatcctta gcaaaaagta tgactttcct
    15541 attcacatag aaaatgaagc aaacttaaca gctttagctg agaatacttt ttcaactgta
    15601 cataactcac tacttagtct cagtattcac agtggatttg gttcaggcat tataataaat
    15661 aataaattat atagtggcag aaatggtatg agtggagaaa ttggtcacac tatcatcatg
    15721 cctaatggta aactatgtcc ttgtggaaat cgtggctgtt tagaacagta ttgttcagaa
    15781 aaaaaggtat ttgaacagct ttcttcttta gaaaatattc ctaaaataga ttctgatata
    15841 gtaaagcaac tatattatga agataaccag aatgccaaaa aagttattca tgagttttgt
    15901 agttacttaa caatagctat aaataatgct attacaactt atgctcctga aataatatat
    15961 ttaaatagcc aaataattag tgatatccct gagatattac agattacaaa agatatgtta
    16021 gttagctctt ttaacaaagg aataaatata gaaatttcat cattagggtc tgaggcatct
    16081 ctatatggtg gctctgccgt aaatatcaag agctttttga atatacaaaa tttaacttta
    16141 attaatgaaa tataagggtt atgactatat acagccataa ccctttaaat tatacattct
    16201 aaaattagat ttattatata gtttatgcaa gattagctat attttttaaa aattgtatat
    16261 caattaaata ttaacataag tatttcgttt taaatattaa tataatcttc ctactcatca
    16321 tcagataata aacttgcatt ttgttcaacc atagaaatat tatctttacc tattttctct
    16381 aattttgctg ccaattcctt tgtatctaaa aaagctgatt caataaccat acctaaattt
    16441 agccctgaaa taattttaat tcgactgtca ttattggcta ctaataaagc agcattgcat
    16501 ggagttccac ctagtaaatc tgccattata attatttctg aactttcctt gtacacttct
    16561 aaagcttttt ttaatttttc tattgttcct tctattccat catcttgttt catatttaca
    16621 gtaggataat ctatagaatc ccctattatc atttttgcag aatttaagac ttcttcagcc
    16681 atattgccat gactcatcaa aataatacta ttcaatttaa tacacctctt taaatctata
    16741 agatgtctac aagataagtt ttcttatcat caggcgtcat tatagttgta atttcaattc
    16801 cttcatctaa aagtctttta aatgcttctt tctcttcttc agtaacactg atattaggtc
    16861 ttagaactgt tgtatctttt ctacttgaca tatttcctac atttatatta tgtattttaa
    16921 atcctgcctc tacaaatgga ataaggtcca caggagactt aacaattaat aatacatttt
    16981 gtccttcata ctttcctgca gtaatatttt ttatagcagt ctcttctgta attatagatg
    17041 acgctatacc tgcaggtgta gccattctta aaacttgttt ttgaacagaa ttgttagcta
    17101 ctgcatcatt tactaccatt aatcttgtaa cacccaattt attactccac atagttgcaa
    17161 cttgaccatg gattaaacgg tcatctatac gaatattttt aatacctttc atttccatat
    17221 ctaatatcat cctttcctta ttttaaaata cctaatgcat ataaaattat tgaaacaacc
    17281 attactccta aaattaagta tataggtttt atattctttt taagagccca ataaacaatc
    17341 acaactagaa ttgcaggaac taacccaggc ataatttggt ctaatatttc ttgacctttc
    17401 attgtaaaat caccttgttg aaagttcaaa gcaacattag cttttacaac tgaaggtatt
    17461 aatgcaccta caaccattaa acctaaaata gatgtactct cagttaaaat ctttaaactt
    17521 ttttcaattg aagatattag cttagtacct gatgtataac cagcattaaa tagtggtata
    17581 cttaataatt tcaatgctac actcactaca atccacagta agattccaaa tggacttcca
    17641 tctatcgcca tattagctgc tatagctcca aaaattgtcc acggaataac gacaaataaa
    17701 ctatctccta aacctgctac tggtcccatc aagcctgttt taattgaagc aacagcctct
    17761 tcagtctctt cacttgataa ctcctccatt gctacattaa tacccaaaat ataaggggca
    17821 acccaaggat tagtgttaaa aaattgtaaa tgatttaata cagcactttg taacttttct
    17881 ggattatctt tgtatactac ttttaaaaaa ggtagtactg cataacagta tgccaaggcc
    17941 tgcatcttct cataatttga agaaacagaa ctagtaaaaa accagcgcca taagacactt
    18001 tttttcacct tttcatttga tttatttaat gataccttat cactcatctt cgtccatacc
    18061 tcctactaca gtatattgtt gttcttgatt ttccaaattc tttttgtaaa ttataagtgc
    18121 aattgctcca ccaattaaag ctacacctaa taatggtact ttcatatata ctgctaaaac
    18181 aaaaccaatt actaagtaac taaaatactc cttagcagga agatatctaa gtaataatcc
    18241 tatccctact gctggaagta gtcctccagc tactgttaaa ccacctgtta accattcagg
    18301 tgtatatttc aaaatggtat taattaaaga aggtccaaat attacagata gagcaactgg
    18361 aatacctgtt gtagctgctg ttaatagtac tcctaacatt tgcatatagg ttatattttt
    18421 ataatttcct tttttagctc catcttctgc tttatgaact agccatatac caatagtatt
    18481 tctaagaaca tctaattgta ccatcaataa agcaactgga atacctatag agattcctac
    18541 agatgcttct tgcttagtcg taatagttat aaatgtggct actatacttg ctgtttgata
    18601 atcaggtatt gatgctccac caaagttact aatacctaat gataatagct gtaatgtacc
    18661 tccaatatat aaccctgtat ttatatctcc taagataaga ccagtaataa aacctgctgt
    18721 tactggttga tatgttccaa acattgttga atttttttca tagtttataa aaaatccata
    18781 tagtacgatt aataataatt gccataattg tggctccatt atgatttcac ctctcccaaa
    18841 atatttttta gttctttaaa attataactg gaatcttgct ttaggaaaat aggaatttct
    18901 tccagtgggc attcaacttc ataagttttc ccaccttcat aactaactcc actaaatata
    18961 ttttcccatg tatgtccttt tggtaaatat acttttctac ttctatcttt ataattcatt
    19021 attggagcta ccaaaatttc atctccaagc atatatgtat tatccacctc ccaagtttta
    19081 tcatcttcag gaaattcata aaataaagtt ctcataactg gtgttccatc ttcatgcgct
    19141 tctttcatca gtttttttaa ataagttttt aagctctctc taatttttat gaattttgta
    19201 agaattactt caacttcttc accataagac catatttcat taggagctcc agttaccatt
    19261 gagcctccac ctttatttga taatggttta ctatgaggca atctatctcc atgcattctc
    19321 aaaattggtg aaaaagtagc atattggaac catcttacca taagttctct aaattctggg
    19381 tctttaggat ttcctccatg gaaacctcca atatcagtag tccaccatgg tatacctgcc
    19441 agtcccatat ttaaccctgt atttacttga ttattaaaag cttcaaaact agaatcaatg
    19501 tcaccagacc atactaaagc tccatacttt tgagctcctg cccaagctcc tcttacaaga
    19561 gttacaacag actctatgtc attatgtaaa ccatcataaa tcatttttaa ataatcaata
    19621 ggataaatat taccacaact taaaacattt ccttttttat atctatagtt atcaaaatca
    19681 tacacagaat agccaggttc agctacatct aaccaataat agtcaatccc aagttgttta
    19741 taatttttat ctattctatc ccaaacatat tctcttgcat tttcatttgt catatctaca
    19801 aagactgtat ttccttgaat ttgcattgtc attctaacac cacgattaac attgactaaa
    19861 tagccatttt ctaaataatc attgtagttt tctgcatcac tttgaactgt aggccataca
    19921 gatactattg gttctactga atactcttct ttcaattttt taactaattc tttaggattt
    19981 ttccaataat ctaagtcaaa tttatattca ccttgcttag gccaatgaaa atagtcaatt
    20041 gcaattgatg aaagcttaat tcctctttta gaatattcct tcacaacatc taaaacttct
    20101 tctgaagttc tatatcttag tttactttgc catagtccta aaaggttttc aggcatcata
    20161 ggaacagttc cagtaacttg agaataattt ttatttaact cttttgggct ttctccacaa
    20221 gtaatccaat aatctataaa atttgtagaa aacatcttcc attctgtcat atttttagca
    20281 aaagatactt ttccaattcc cggattattc cataaaagtc cataacctag actagaaata
    20341 taaaaaggaa ctgatatttg agaatttctt tgggctaact ccaaaacagt attctttaaa
    20401 tctaaaaatt tatgttgata ttgtcccatt ccaaaaatct tttcagaagg ctcagattca
    20461 aatctagtag ttacttcaaa ttctccacta tagcttggtc gatattctct tgattttaac
    20521 tttagagtag agttaaagtc tttggtaatc tcaattgtcc ctacatcttc tccaccatca
    20581 tcatgtttta cagctcttaa tcgaatatat tctttcaata atatttcatt tttatcatta
    20641 taaaaagtaa ttctatctct atggtctaag acagctttta tctttccatt tttaatgatt
    20701 gcacttccat cttcattttt attaattgta atatcatctt tatttaactt aggtttttcg
    20761 ttaagagcat agttttcatc aacaaaattt tggtctacaa acgacctaat acgtaaagag
    20821 ttttttcccc atgcttctat tcgcaatagt tcattgtcaa atcttttttc aatatagtct
    20881 tcagatattt ttatcacggc tacctcctaa atcaatggta taatattcta tctttgtgta
    20941 gcttaagtta aataatgtcg tttagtatcg ttatttataa attaggtttt atatataatt
    21001 catttgtaca ttaaactaac ttaccaataa tatactttat ttgactttgt ttgtcaatcg
    21061 ctttccggat tttagtaaaa ttttctaata tgttgcttga aagtcgttct taatatcttt
    21121 tatttatact tttaatatct gtgatgatac ctatatacat ctagtttttt taaaacacat
    21181 acaggaataa agtttgccta ttatgtagat aatatcggac ttgaaaatat gtatagtgac
    21241 cttataaata cagataatat attagatatt gttcaaaaca atggatttta taattataaa
    21301 taatttatga aattatttaa taaaatatat aattgtatac caagtcaaaa acgacaacaa
    21361 ataaataaaa ctaaaaaaga tgttgtctca aaatatagat aaatttattt ttcattgcta
    21421 aaatgattaa aatatatttg atttaatata cttgatttaa tataaaaaaa gagtgtctca
    21481 taaactaaaa agttcacttt tagacactcc tttatatata ttatctactt cttaattcat
    21541 ttattacctt tctacatttt ctttcagcta gtggatacat ctttataagt actaattact
    21601 ccacttaaaa atggttcctg tactgtccat cagtcatatt tttctgccaa atataatggt
    21661 gtttcataat gagacaataa atatttatat ctctacttat cttctctaag ctcgtcaaat
    21721 ccaactatag gtatctctaa attatattct ttaagtagct tttctagctc ttctataaaa
    21781 atacctttcc catccaccat ttgtatatca tttattttat ccatcaattc attgcaacca
    21841 cttaactcac caaaccaacg tttagaacta catgtcttat tataaccaca acttggactg
    21901 ccatctattg caatgatact ttttatatca taatcatttt ttaaatattc atcaaactgc
    21961 cttacatgag gtattaacat ttgcctacat tgttctctaa aaaacaaatt gtcaaactgt
    22021 tcttttacat gtccccacct atttattcca tacataatca tttctgggca tggtagttga
    22081 attattccat accctttatt tattaaattt atcgctatat tttgtgctcc cttaaattct
    22141 gacaatccct ctactttaga attacaattt aatatacaat gacatactac tacaatctct
    22201 ctatttctct tcataataat ctccttagtt attcttaatt ttaatgatac tacatatttt
    22261 gataattata ttatttattt gttctatatt tccaatattc tatatagaaa tatacaatta
    22321 gtaatctttt ataatctata ctatgataaa tgtatatatt tttttaggag gtattataat
    22381 tggatactaa aaaagtcaca ttttcttcaa tgtgcatagt tattaatatt gtcttaggta
    22441 tggttatatc tatgataaat ataccattat tatttttaga tacagttggt actgtacttg
    22501 gggctgtcgt attaggtcct ttttggggtg ctcttattgg tggatgtaca aacttagttt
    22561 taggtgttat atcaggtcct acaaatatac cttttgccct tgtaaatatt gttcttggtc
    22621 ttattgttgg atatatttct aagaaaaaag gttttggtta taaagaagct attattacag
    22681 gagtaatttt atctattgtt tgtcctttaa ttggtactcc tatttcagtt ttattgtttg
    22741 gtggtttaag tggaagtggt gctgatttat tggttggttt tttagttcag tctggtcaga
    22801 agatatttac tgctgcgttc tttccaagga ttcttagtaa tatagtagac aaacctttgt
    22861 cttgtattat ggtggtatat tttataagta aaatgcctaa aggttttata aaacaatata
    22921 gtaaaaatac ttctagttca tattaataaa tcatttatta tgtaaggttt attactcttt
    22981 aaaatgctat atactcattt ttatataaca tttttattaa taatttaatt agatagtatt
    23041 tgtattactt taattaaacg taacctcttg atataaattt gagctataaa tctatatcaa
    23101 gaggtgctat tttattttta atataattaa tattctatta cgcaaaaaat gcatatattt
    23161 cattatttta tggaaaatat tacttcattt aactctatat caaactcatt aagtaaaaat
    23221 ctaattatat ttaatttagc ctctgtattt aaatttgttt ctaaatatat attttctgat
    23281 attttaatac cttttcttaa tttatatgca tctctagact taatattatc tataaaatct
    23341 cttttataag tcttgtctac aatactgtta aaccttacag aatccaaatc atataaagta
    23401 agagcaactt gttcataaaa ttctgtccaa tttttagcct tatatgactc tcctaatata
    23461 ctaaatgata ctatactttc atttgtaaaa ttaacatcat catcctctaa cgtaaatata
    23521 ttttcactgt tacttggagg ttcatatgta acacttggat atttccaaat ttctacagct
    23581 ctatctagta atatcttagt tctatttttt atttcttctt cattccattt atctatagat
    23641 gaaatatatt tatttagata aagcctacta tctttaaatc catcttccat ttctttcttt
    23701 tcttcaaatg ttttattact tagttttgaa ttatatcctg tcaaagttaa atttccaatt
    23761 gtatgtaaat atttatcatg tatctcctta tagttatctc ctagtgattc cttccattta
    23821 tttgttaatg tctgtggcat tatatgctct atatttagca tattattatt gattaaattt
    23881 tctaaatcaa caatttcttt attattataa ttctctaacc tctcaagtat atataactta
    23941 ttctttgact tagtattata tatatcttta gtttcaaaag catttgaaaa ttcactgtca
    24001 gtaggaaatc tttgagatga tttcttattt ataaaaatat atcttaatat attagaatag
    24061 ttttctttat aatcactaaa ttttttaatt tcccttccta agttcataaa tactttattc
    24121 agtgcattcg gaggtacatc acatactatt cttcttaata taaaacattc tactaaatta
    24181 agggattcta caagtacttt tttatttatt ataccttcat tataatcatc aaataattct
    24241 aatataaacg gatatgatac aaatatttca agtttgttta tccttttcaa aactttgttt
    24301 acatcattat catcagtaca tgaataagat atctctctat aatagttaga aaatttaagt
    24361 aaatctttta ataaaatttc tatatctata ccattatctg atacatacct tttaaaacta
    24421 aaatatactt tatttttatt cggtatactt ctctccttta tagtcaaata gtctcttata
    24481 aaactactta cgttgctact tgtatctttt tcaattttat accaataatt attataaaat
    24541 tcctcttgta acttagagct tctacccatt aaaacaaagt ttcttactct atcagcttca
    24601 cttaaatcta agccagttga atttagactt tcaaagatta attgagggtc atcttcacca
    24661 ttttttaact ctatttctac tatagttaat ttctgtatag catcaaacaa atcatctatt
    24721 gatatattat cttcttttat tctattataa aaatataaat aatttgaaac tatattagaa
    24781 tttctatctg tattcatttc ttcttcaaat agttcatagt acgcttttct atcttctgct
    24841 attggtttta actttatttt tccatcttta tctcttgagt gcttgtttat aagataacac
    24901 tccttaatct gttcttctgt tgtaataatt ccttttgatt ccccaaattc ttttattatt
    24961 tgacacatgg caatcaataa tattgacaca gtagtcactc tttgttgtcc atctattatt
    25021 aaatattctt ttcccatact atatatagat acaactgacc ctaagaaatg agttctaaat
    25081 ccagaatcta ttatatcctc taaatcatca aacaatcttt tacattgttc tttcttccag
    25141 ttataatttc tttgatatac aggtactaca aaacttttgt ttgacccctc taaataacta
    25201 agaaattcaa tttctcctgc tttcatatta actcccccta taagttaaat aaattccatt
    25261 cataactcca tgtatctata tattatatca aaattttgac tcataaatgt atatgaaagc
    25321 cttattatat acctttttat acctgttttt attttattaa agtaaataca tgattattct
    25381 ataaaatata caaatactta attttaaaaa ttattattta atcttcttca tgagtaattc
    25441 atagttattt atcaattaaa aagagatatt gcataattat tcaatacctc tctttgtata
    25501 tttacattat ccatatctag ttatctattt caacccaata ttagactacc tactaataaa
    25561 tttattctct acatcattaa caataatttt tcactaaata acatcattag cctatctctg
    25621 atgcattttc tttaatttta tttatagttt taatcaattt atttctctct tcaatatgat
    25681 tttcaaataa atcaattata cttccaacag ttctaaatgg gtcttccatc aacactttgt
    25741 tatcctctat aaatccattc ttgactacat aatcgactat aagtttaaca aaatgtattt
    25801 gcttagtatt aaagctctta ttatttaaaa actctgaaaa agcttcattt gcagcttctc
    25861 tatccaatcc cactagtttt ctcactaatt gacttactgg agtatctcca aactccttct
    25921 tataatcatc acaattacct aactgtttta agagtatttc ctccaacgtt tcaacatctt
    25981 gctttgttat ctttttatta tttcttaatt taaatattgc taattcatct ttatggctat
    26041 ttaaataata ctctaccttc tttttataat tcttaagatt gtttgcatta tatacagaat
    26101 catttctatc ttctgctaca atcatatctt caaaacttgt ataatatatc ttttgactag
    26161 acttttcaag atacttaata agctctctta atgatatacg tacttcttct acctcaaata
    26221 tatctgaatc ttcccaaaaa tcctctgtca ttgctttttc aatagtatat ttttgttgct
    26281 gaatctgtgg tatagtccca agttttgaaa gtttttctgc tgtagccatt acatgcctta
    26341 tacctttact tgcattatta ttttgtaagt atgcaagttg tatattatac ataactacat
    26401 caaatctttt agccaattca tcatcactat atgaagttat gagtggtgat atatattctc
    26461 ttatctcatt ttgtgctact gctccaatat tgctccaaac attttcattt ttatacttat
    26521 gtacataact taaattcatc ctaactatat aactatcctc atttaaagct tttacagaag
    26581 ttataagttc ttctaacaat tcttttctaa gttctgcata ctcaccttct atatatttaa
    26641 tatcttgaag ttctttaacc atacttacct ttatattaaa taatttttca gtaagtgttt
    26701 ctgctttatt tccctcaaat cctttaggat taaatttaaa aaattcaaaa ttattacaaa
    26761 aatcaaatat taagaatctc tccttatcca tatcaacacc taataaatct ggacataatc
    26821 tagttcctct acctatcatt tgccaaaact tagtcttact tctcactttc ttgaaaaaaa
    26881 caagatttag tatttcaggt atgtcaattc ctgtatcaag catgtctact gaaactgcta
    26941 tttgaggtaa tttattctta tcagaaaaat catctattat agaatctaca taatttatac
    27001 tatagtcaac tgcttttaca aagttcccac catactttgg atacagttta ttaaatcttt
    27061 caactattgc cctagcatgc tttgtattct ttgcaaaaat tatagtctta ccaatctttt
    27121 cttctccttc tattttcaac ccctctgtca ttagcttatt taaaactaaa tctattgtgt
    27181 cagaattaaa tagccactca tttactgcat tattccctat ttcgtctcca atactctcat
    27241 catcattaaa tgtattttca tattcttcct tctcttcatc agaaagctca tcatacttaa
    27301 ttccatcttc cataatctta gtcttaaact caatactagt ataatctact aaatatccat
    27361 cctcgactgc cttattatac tcataagcat atgtaggtac tccattttcc atgtcaaaaa
    27421 ctgaataagt atttttatct atctcatcct ttggtgtagc agtaagaccc attaagtaag
    27481 aatcaaaata atcaaagatt gacttatact ttttataaat acttctatgc gattcatcta
    27541 ttataattaa atcaaaatga ccaggtgtaa acaatctctt tccatcctta gacttcgtat
    27601 catcaattgc attcatcatt gttggatatg tagaaaatat catcctagca tcttctgggc
    27661 tatctttgct atccaataaa ttacataacg ccaagttagg taatagctta gtaaaacttt
    27721 ttttagcttg ctttaccaat gcttttctat ctgctagaaa taatatattt ttaatccaat
    27781 tatgtcttga aagtacatca actatagata tagcaattct tgtttttcct gtaccagtag
    27841 cacataccaa aagagttttg cgttgcttat tttcaagagc ctcacataca gccctaatag
    27901 cttctttttg ataatatcta ttggatattt catcatttat tgttacagaa gttaaagact
    27961 tcttactctt tcttctatct atcataagtt gtaattctga ttttttataa aatccataaa
    28021 ctttcctatc tgaatagtca tcccaaatat acatatcaaa accattagtc aaaaatatta
    28081 ccggtctttg actatatctc ttttccaaac aatctgcata taatttagct tgttgttgac
    28141 caattttcgg gtctttactc gttcttttcg cctcaactac tgctaatggc ttaccatttt
    28201 caccataaag aacataatct acatatccta caccactttc attaggcata ccttgtactt
    28261 ctatttcttc ccatatatct tttccaaatt cccaacctgc aagctttaat tctatattta
    28321 tatatctttt tcttgtctca aattcgctta cttcgtctat cttgaaatca tagtttgcag
    28381 tattaacttt tctcttttca gttagtgact ttcttaattc ctcattttct tttattactt
    28441 cttcaagctt tttatctttt gaacttaatc tctcataaag atttttaagt tcgtctggtc
    28501 tcgtcctttt ctcttcccca tgaagtaata aatcttcatt aaagtcttct gctgtatatt
    28561 catctgaata acaatagtct atccaactaa caaattgatg caaattgtta agtgaaagta
    28621 ttgcttctcc tctctctatg ttcgcactag tatgtacaga taaattacct aatttaacta
    28681 tatattttaa taaaggtaac atatcatagt ctataagttc tataaaacta ttattatgaa
    28741 tcaacgtact aagattatct tgataaggca ataataaatc actatcatta gtatatagcc
    28801 attttactgc aagctctaaa gctcttcttg ttatagttgc acaagtagat gggctaacta
    28861 gtatactttt ttctgcttct aagcaagcag tattaaaact attgaaattt ttcttctttt
    28921 gtaaaaaatc aaaattaccc aaggcttttc tccccctttt gtcccttagt taaaataaat
    28981 ctctctaaaa actatttctt acagctgttt cttatactaa caaatacttc ttcattttct
    29041 gtaaacaatt cgtacatatc ttcaaactgt ggtatatcat atccatgaga atcatcattt
    29101 ctcaattttc taagtgtttc tataactata gaagaatttt taggtaataa attgtgatac
    29161 tcacaagtta aaaggtggtc ataaaagttt ttagatttta tatttaaatc aattgattta
    29221 taatacaaca ttgcttttgt taattctaga aatgatacca tatagctttt aaagccttct
    29281 tttatgtatc tagcccataa ctcaggcatt tcttccttat catataagat tttgaagttt
    29341 tgcaggtcat acaagcaaga accaaaatcc ctatttactt cttctattga gttccaagat
    29401 aatttgtttc taattaccat acatgtccct ccaatctaag atatctatta catcactgtt
    29461 ttcaaaatag aatttattta tataatcctt aaaatcttta gattcataaa taacttcacc
    29521 ttctaaaata gcttttttaa gtgctattgt ataattatct gtaacaattt ttaggtctac
    29581 atcaatgtct aatatatttc ttagagcttt tgtcttagtt gcaagttctg taattggtac
    29641 ttttttgata ggaatccaag ctatgtctat atcactttct gatgtatgtt tatctgttcc
    29701 ataactacca aatattatga ttgctagagt attttctttt ggaaatacat ttattattga
    29761 gtttttaatc tcatttggta tatttctcat ttaatcacct cttgtttatt aggtaatatt
    29821 gcttaattga atttatatcc ataatctttt aatctattta tacatatttc tctttcttca
    29881 tcagtaaaca actctttata cttttctttt aatactaata cttctaatga taaatctaat
    29941 ctcttacact cccatagcct tgtaaatcct tcagtagctc cttctttatt gattaattct
    30001 ttagcagcta ataaggctcc tttgttattt atcagttgta aaaatcttgt tggcttatag
    30061 ttacattgta aatcagcttc tttatagata tttaccatgt catgatataa atctagctct
    30121 atttgattca tagattttat tcccccattt taatacttgt attttagttg aataactctc
    30181 ctttaaaagc tttttgcatt aatgagttaa agttgttttc tagttctttt aagctatctt
    30241 ccattcttga tttcaatttg tctatttgtc tgacaaagtc cacaaactta ttttgaagat
    30301 atacaggtac tttaggtatt tcaacttcat ttaatttgcc tactgtaaga cctggttgtg
    30361 ctgctccagt tgataatcga tttaaatcta actctttaag tgtaaaatat agccagtaac
    30421 tatttatatc aactttaggt tgtactacaa ctgcatgttc tgtagcataa aactttccat
    30481 ttacatattt tacattacca cataatgctc cttgcctacc tatgagatta aatgttccct
    30541 tatgtgaata catttttaca tatcccctta aaccatttcc tccataacaa ggatataatt
    30601 catgtgaatt tttttcataa atatcttttg cttttatatt ttttccagct tttaagtttg
    30661 atatctctcc taataactct gtatcccaat ctttactatt agtaatcaca tttccaaaca
    30721 tctcaataaa tcttgattta actaattcat ccaatgcttc tatttgctct tttcttttat
    30781 ttattaactc ttgagcttta tttaaaactt ctaccatttt attttgtata caaattgtga
    30841 aatatggtat ttttatatca tttaacttgc ttttatttaa agttgcaccc ataactgctc
    30901 tatcagtatt attaaaattt aaagttctca aagcataata taaatattca tatgttataa
    30961 gttcattatt ttttatttga aagttagcaa ttgcttcatt tgtaaacatg tcttctttta
    31021 atatggcaac tctacctata gacaacttaa aactcattac aacggtattt ttaggtacta
    31081 atttcatatt agctttttca acagctaaat ctgttatctc ttcttttgat tttaatatat
    31141 atttttcttt caaatcagaa attgataacc atctattacc acttccccaa taatcagaag
    31201 tatttctaga tggtgttttg ccaatattta tatagcacaa ttcatttaat tttatatact
    31261 ccatacccta tacctctaac attctttcta attcttctaa cccaacagct atttccttct
    31321 ctaactcctt aaccctacct aaaataacat taggagcatc ataaattact tcttcataca
    31381 ctacctcttt atacttatta atagataaat catacccatt ctctcttatc tcttccacat
    31441 caacaaaaaa gctttgttct gttctttttc tatctacttc cccatcaaga ttatcaaatc
    31501 tcttaataat atcctctata tcactatcct ttatctcatt tctcttatca tccaaagaat
    31561 aaccatcagc tttcatatca tagaaccata ccttatcagt tccacctgtt cctgttttag
    31621 taaatatcat tatagctgta gatactcctg catatggctt aaatacacca cttggcattg
    31681 atattatagc ttccaattta tgattatcaa ctatttcttt tcttatcgat ttatgtccac
    31741 ctgttgaacc aaacaatact ccatctggca ctatacatgc gcatcttcca ccaattttta
    31801 aaactcttaa aaaaagtgcc aagaataata actctgtttt ctttgtctta gtcactttta
    31861 ataaatctgc tgacactgct tcataatcta aacttccctt aaaaggagga tttgctaaca
    31921 ccaatgtaaa cttatcttta tctgtattaa cctccgataa agaatcctta tactctatat
    31981 taggattgtc aacgccatgc aacatcatat tcatagcacc tattctaagc atagttctat
    32041 ccatatcaaa tccatagaac ataccattat taaaatgctc cttcagccct tgcactaaga
    32101 aaagacttga atgattatcc cttaagtact gttgtgatga tactaaaaaa cctgccgaac
    32161 ctgctgccgg gtctactata atatcttctg gtgttggttt aataagtctt actatcatat
    32221 ctattatgtg tcttggagtc ctaaattgac cattagtacc tgctgtagcc actttggata
    32281 ataaatactc atacaaatct ccctttgtat ctctatcctt acttaattct aaattactaa
    32341 taccatcaac tattttagat aacataagag gcgtaggaat tttaaatatt gcatctccca
    32401 tgtactttgc ataagctgag tctttatttc catgtaattt ttttatgaaa ggaaacactt
    32461 cattttgtac tatttcatac attttttctg aatttccttc attagaaaac ttactccatc
    32521 ttaaatgttg atgtccatca tcaaatatcc tttcaaaaga aactcccaaa agagttgctt
    32581 ctgcttcatt ttttgtttct acttcatcca atcctttaat aaataaaaga tatgtaaatt
    32641 gttctataac ttctaatgga ttagttaatc caccagtcca aaatgtctcc caaatcttgt
    32701 ctactttgct ccttaattct cctgtaatca tatatgactt tccctccagt gttcataaaa
    32761 taatttaaat atatatttta gtcgtacgcc ttaataatta gtgtcttata ttaaattcaa
    32821 aattttacta cacagttatt aaattatttt tcctatttac atgttttagt taaaaaacta
    32881 atgcaatact cttattttat gattattata catcccatac aaaattattt tatcatatat
    32941 ccagcataga agtcatctac ctggataact cttaccatat tataactttt taatattaat
    33001 ttaaacttag aaagaaattt tttatttaat tatatcatct tccttatttc aaatttctca
    33061 attataatct taatcaaaaa ataaaataag atgctgtctc aaaatagata taatcttctt
    33121 ttttattttc ggaatgatga aaatatattt aatttcatac aaaaaagagt gcctcctgaa
    33181 ctaaaaagtt cattttgaga caccccttaa tttaatctta cttattcttt ccctttatta
    33241 ttaacacttc actatctcta ttgcttttcc tacacatcca caaacttcca tcctactctt
    33301 aacaaaatct ttaactgact ctaaaccttt tttaccatat ttcttagggt caattacctc
    33361 tgggtcagaa ttaagcacat tcttaactcc attgctataa gcaatcctta attcagttgc
    33421 ataattaacc ttgcaaatcc ctctttttat agattcttta ataacttcat ctggtattcc
    33481 agacccacca tgtaatacta atggaattga tacaacatct ctaatttcag ataatctatc
    33541 taaatcaagc tttggctctc ccttatataa tccatgtgcc gtacctattg ctactgctaa
    33601 agatgatacc cctgttctct ctacaaactc ttttgcttct aatgggtttg tatatgctcc
    33661 cccatcacca ccatctaaat cgtcttcctt acctccaact ttacctaatt cagcttcaac
    33721 aggtatatct gaaggcatac atgcatctac aacagattta gatattgaaa tattatcttc
    33781 ataactttca tgagaaccat caatcataat agaagtatat ccaactctaa gtgcctgcat
    33841 agctagtcca aagcttgaac catggtctaa gtgcattgcc actggaacac ttgccttctc
    33901 tgatgctacc tttacattcg ctaaataata atctagtcct gcatacttta ttgttgaagg
    33961 tgttgtttgc aatattactg gtgatttaag ttcttcagca gcctctatta ctgccataac
    34021 catctccata ttttcaacat taaatgcacc tactgcataa tgcccttctt gtgcatctaa
    34081 taatatttgt ttagttgtca ctaatgccat aatttctcct cctatatgtt tatactgatt
    34141 taatttcttc gttatttaat tttgatacta tttctctact tgtttcgcta tttaataact
    34201 catttaaaaa ttcttcattc attaattttg aagatagttg agataatgtt tttaagtgta
    34261 aattatcacc attatctgta gctgctatca taaatataat atgtgccaag cttccatctt
    34321 ctgattcaaa gtcaaaccct tcttttgata cacctactgc tacacgtggt acctttactg
    34381 catctgtttt tgcatgtggt atcgctatcc ccataccaaa acctgtagat gatatttctt
    34441 ctcttttaag gatttcttct ttaaacttct ttttatctaa aagaacacca gatttatcaa
    34501 gtattccaat catttcatca attacatctg ttttattttt tcccttaaca tctaaaatta
    34561 ctgtttcttc gctatataaa gagatattat tagatacgtt ttccgattct gcactttcat
    34621 ttttatgttc atcttttgtt gcatttgtat tttctatagt atttttctca tttactactt
    34681 taggtgtaac catcatataa agtacagcac ctactaatga acctacaaat atacacccta
    34741 cccataaaag tggcttattg actaatccta gaaccaaaaa ccctccatgt ggagctggta
    34801 cttctatttt cattatataa gttaaaattg atgatattga tgatgcaatc ataagtactg
    34861 gcaacacttt taatggattt tttgctgcaa aaggtattgc cccctctgtt atatgagtac
    34921 tacctagtat ataattgatt aatcctgctg ctctatcttc ttctgtaaat ctatttttaa
    34981 ataaagtaga tgctattgct attaccaatg gtggtgttat acaagctgca gatacacctg
    35041 ccataaataa ataatttccc tgtcctagaa gcatagttcc agttacatat gctgctttat
    35101 ttactggacc tcccatatca aatgctgaca tacatcctat tactattccc aaaattatag
    35161 ggcttgaatt ttgtaacccc gatagaaagt tcatcatagc attgttaagt gctgaacatg
    35221 gcccaccaag taacaacatt gctacaccca caagacctac gctgacaata ggaattataa
    35281 aaatcgattt aagaccttct aattgtcgtg gtaattttct taacaaatgt acaaatagtt
    35341 taactaagta acctgctaaa aatcctgata tgattccacc taaaaacccc gaacctgtat
    35401 caaatgatat cattcctcca acaagtcctg ccaacatacc agctcttccc gatatagatt
    35461 gagctataaa tgctgccaat acaggaacca tcaagttcat agaatatcca ccaacagatt
    35521 ttaatgtagc tgctatttga ttatattgtt ctgaatttgg gtcaaatgaa taaatccccc
    35581 ataaaaatga tatagcagta agtactccac ctgctactac taaaggtaac atgtgagata
    35641 caccattcat taagtcttta tacaactttt gaccaaacga taaatttcct tcttctgcaa
    35701 ttttactgct tgataagttt ccttttctaa tagctacttt tccatcaata cacctttgta
    35761 taagttcatt agccttatgt ataccttctt tgacaggtac ttctattacc ggctttccat
    35821 tgaatctgtc catatcaaca tttttatcac aagcaactat tattgcatct gccatttcga
    35881 tatcttttgc tgttaactta ttttctacac ctatagctcc attcgtttca acttttatgt
    35941 cacagcctaa ttccctggct gaatttttta atgcttcttc tgccataaaa gtatgagcta
    36001 ttccagttgg acaacctgtt acccctagaa tcattttctt tcccattttt ataccacctt
    36061 tctgactctt atatttttta tataatcttc cacattctcc aaattaccta taccattact
    36121 ttcagccaca cttgctcctg ttgctgctga cctttttaat gcttcttcaa tatgttctgg
    36181 attttccaac catatactta aaaaagcagc taatgcagaa tcaccagcac atgcagagct
    36241 gactactgta actggttgag cactagcata ataaattgat tttccattat aaaaataaga
    36301 acctttttct cctaaagtta gcaatatatt ttgagcacct tttaaatgta gtaatttaag
    36361 tacatcctta atatcttctt catctcttac aataattcca aatatatctt tgatttcttc
    36421 atcatttggt tttatcaaat atggattata ctgtagtaac tcctttaatt ttggtgaact
    36481 tatatcgagt attgtcttta tacttttatt tttacaaatt ttaaatatat cctcatagta
    36541 attatcatca ataccaggag gtagacttcc acttattgat aagtaactta tatctgctaa
    36601 tgactgtatc ttatctagca tatctagttt ttgtttttca tttacatatg acccagagtt
    36661 tacaaattta aattcatcac ttccatcatt taaaaatata tttatacgag ttgtatcttc
    36721 tacccaagta ggtaatacgt caaatcctct cttttcgctt tcttcaacta tatattttcc
    36781 tgaaaatcct ccaaaaaatc ctaatatctt agattctata ccataatgac ctagtacaaa
    36841 agttacattt aatccctttc cattaggaga atatacagca tttgatgtac gatttactaa
    36901 ttttctcttt atcccattag tagatatatt catatctatt gcaggattcg tagttaatgt
    36961 atatatcatt gtcttccccc ttgctcatga tgtgtatgtt ttcattataa taaaaaagtc
    37021 tatgaaaatc ttttcacaaa tagaaaatcg attttcatag acttttcaat ttaattttca
    37081 gaattattat atctgaccat ataatctata attatcctag atataatata caagggcaat
    37141 ctggaagata cttcatattc agaaattgat atatgttcat gtttgtatcc tacaatatat
    37201 aaatccgaat actttataag ttttgcactt tcactacaac agcaagttat aaccttagct
    37261 ccaactaaat tggcattctg tgctgattct acaagctcta tggtatttcc attcaatgaa
    37321 aatataatta taagttcgtc tttttttatt gatttagatt ttattttcat tatgttgggg
    37381 tcatttataa aaacaacatt aaaatcaagt aattgcaatt taagactaaa ctcttttgct
    37441 acatattcag taagccctct tgccaaaata tatatcttac tagatttatt tatagttttt
    37501 attatattga ttagtgtagt taaagatatt tgttctataa ctctctgtgc ttctataaag
    37561 gatttattta ttatatcccc catattttgt atatcatcat tttcatcttt tactgttaat
    37621 ctatacctaa gttcattgaa tccatttaaa ccacattttc taattgctct tgaaacggtt
    37681 gctggagatg aatatgtttc atgagcaatc tctactatag ataattctgg taatattgat
    37741 tcattttcat ttataaattt aattatttca agttccgtct tcgttagatt ttcagaatta
    37801 cttttgttta gctcaattat catatgttat ctccccttat ataagtttta tttgtctaaa
    37861 tttatcgagt aatataatat attacctctt aactacttat ttttatatac aattaagatt
    37921 tagtcaatgt ctatatatgt aaatttataa tatcataatt ttttgcttaa agtttattat
    37981 gttatcttat agcattttaa aataaaaaag aattgtacct ctatcaaaat ttggatgaag
    38041 tacaatagat attcctcata ctgatatgtt tatgattaaa agtataaata taaacttcgc
    38101 tatgaatcat ataattaata aatcatttta tttacaatta taacccccct tataaaacta
    38161 tattaataat atatatagta tttataaggg gggttgttta tatttaaaaa ctatttactt
    38221 ttctcttaat tcttctccat tagttcttat tacattttga taccaataaa aactatcttt
    38281 tttataacgt tctttacttc catttccatt gttatctata tctacatata taaatccata
    38341 tcttttttcc atttcttgag atgaacaact tactatatct attggcgacc acatacaata
    38401 agctaatatt tcggttccat cagctatagc ttcacctatt tgttctatat gttttcctaa
    38461 ataatctatt ctataactat catgaacttt tccatcttca aatttatcat gcattccaaa
    38521 tccattttca gctatcatta ttggcttttg gtatctatca taatattgag ataaagtatt
    38581 gtataatcct ttagggtcaa ttgcccaccc ccaaggattt gcttttaaat atggattctt
    38641 tatattatca gttggattca tggtgttttt acttgctgat actgttgtag aataatagta
    38701 tgataaagct agaaaatcca tagtattttc ttttaagatt ttatcatctc cttcctccat
    38761 aactatattt atgttatttt ctttgaaaaa tctcttaata tagactggat actctcccct
    38821 aaaacttaca tcagtgaaaa aatattgtag cctatttctt tttaaagcaa atactacatt
    38881 ttcagggtca caatcttctg gataagatgt acaatctgcc atcatcatcc ctatcatcat
    38941 atctgaattt atttcttttc caagcttctt agtcaaagct gtagctatga attgatgatg
    39001 aatagcttga tatctagcct cttctacatt atctacttgg tctacacaaa atgcaactga
    39061 attaaatgat tcataatgta ttaaatttat ttgatttata actatccaat atttaacttt
    39121 atttttatat ctatttagta ttaattctgc atatctaaca aagaaatcta ccacttttct
    39181 attagaccat cctttatatt ttaatactaa attcaaagga gtctcatagt gaagtagtgt
    39241 tacaataggt tccataccat tatctattac ttcgtttata agattatcat aaaactttaa
    39301 tccctcttca ttaggctcta aatcatctcc atttggaaat attctagccc aatttattga
    39361 aaatctaaaa cactttaacc ctagctcttt caaaagcttt aaatcttctt tgtatgtatg
    39421 ataaaagtct attccactac gcttaggata atatccatta ttatctttca tagcaaactt
    39481 tattctttct atagtcatat cagactcata aggtttagat atatctctat ctttatcata
    39541 catatgtaca tcagctaaac accaaccttt gccaccttca ttccatgcac cttctaattg
    39601 attagctgct acagctcctc cccataaaaa acctttagga aacttaccca atttaatcac
    39661 tccttatttt atttatttaa atcatttact attatttacc ttatattcta tagactttac
    39721 tatacctact aaagacatag ttatatttaa ctcgctgaat atggtcataa gagtatcttg
    39781 agcatgtgca aataacagcg aatgtgctct tttctctcca ccagcttccc cttgtataat
    39841 cttggtttga gctctatgtg cttctgtcat aaaccctttt gctttagcta gttttaaatc
    39901 ggcatttcta taatcaaact tttccaaatc attaagagca tgtttaattt ctgttctagc
    39961 ctcacctgca cgaagtatta tttgcattgc cacctctact aattcttcat ctattatatg
    40021 ttcttcaatt gtcatattta taatttcctt tcaatttcta aatttaaacc tcttgactct
    40081 ctaattttat ctcttctttc tcataagctt taaaaaatgg ataccatatc ataaatagca
    40141 atactatatt tattgctaaa acaataagac ctctaacatc ttgatttacc ataaaagtag
    40201 atataccaaa aggaacatac cataaattaa acaccttact tggaattgta acaaatccca
    40261 tactcattga taaataagta gttattgata ttactactgt tcctatccac ataggtaaca
    40321 ttaaaaatgg gttccatgcc actggagctc caaatattac tggttcattt atattcatta
    40381 tagaagggac aatacatgct cttcctattg cttttattct tccaactctg gataaacaca
    40441 tcattataac taatggtaat gtagcaccca accctcctaa agttatccaa cctgtttgaa
    40501 taacttcgta aacatatata ttagttgcta ctcctcctga tgctactgct gctgcattag
    40561 ctgcaattgc accaagttga attgaataag ttataggaga tagtacccac ccacttattc
    40621 ctaaagaata tataaatact actaagaagt ttaagagtac aaatccaggt aatgtctgtg
    40681 ctatatctga tactggttta aatactctaa ctattatttc aaacatgtca aagttgagtc
    40741 cataaattaa tccccagcct acaaacatta tcactgttat aggtaacata ttatcaaacc
    40801 acttgcttac aaaatctgga agtgctgaat cttctccgaa aaatgaaaaa gaaccaaata
    40861 aagacattat taatccagag aataatcctg caactagcga tacaagcatt ccttctcctc
    40921 caaacctgtt gaagttaaat atagctcctt cattcgtaat ctctggacga atcatcatta
    40981 acattaatcc agcacctgca agaccagcga ctacactcat actttcaaat ttcttattct
    41041 tcattatttc cataggcaat aaaaatgcta taaataatcc taaaagtcca aatgtatatt
    41101 gatttattgg acttaaatca ggtaagaaac ttatgtaatt cttcaagaca gaaactatag
    41161 ttactaaaga acctacaaat accataggca aaactttcat tatagttgat tgaactgatt
    41221 gtacccatat attcttagtc atattattta caacaggagt aaatttttca cttaaaaaat
    41281 ctataaattt attcataaac actctcccca taaatatttt atttgtttga tattagttct
    41341 agtgcttctt ccaaagcctt tgccccatct agtcttccat atgcagtttt agatatacat
    41401 gctactggaa cgttatattc attagccttt tgttgaattt cttccttcat ataatctaaa
    41461 tgtggtccta gtaataaaca atctatttct gataaatact tatctatctc tgactcactt
    41521 ctcgctttta tatctgcttc tatacccttt ttctttgcac tctttctcat actagttgcc
    41581 ataaaacctg tacttgctcc tgaaccacac gccaaaagca ctcttatatt tttcataatg
    41641 atacctccaa tatttttatc cattaattta tttcttaatg aactttttat tttattatgt
    41701 ttatattgta cttaccaaat aagttcattt cacataaata tatttcctaa taataggaaa
    41761 tcagttactc ttaattaaac atattaatat agtataatgt atcttataat acgtacattc
    41821 aattatataa ttatcatatc atccagattt tattttaaaa ctatgcgagg gaatctaatg
    41881 aaaacatcta aaaaagaact atttttaaat ttcataaaaa cacaacatga ttggatagat
    41941 tcatctacat tggctaatta tcttaatgtc tcaacgcgtt caattagaaa atatgtaaac
    42001 gaaattaatt ccaatggtga atttatatta tcctctaaaa aaggatataa agtaaatctc
    42061 aataataatt gtcaaaccaa ggttgatagt tcagaaaata tatcacctga taacaggtta
    42121 aatttaattt taaaagaact tatagttaat agtaatggaa ttaatatatt tgatttaagt
    42181 gaagaattgt ttgttagtcc agctactatt gaaggagata tagtcaaagc taataaattt
    42241 ataggaagtt ataatttaaa gataaagcaa tcgaaatttt tattgaaact aattggaaat
    42301 gaatctgcaa agcgcaagtt aatgagctct ataatattta aagaaacggg aagtgatttt
    42361 ttaagcttat ttgatgtaca aaaaatatat caagagtata atttgactaa actaaaagaa
    42421 aatataatat atatattaaa aaaatacaat ttatttataa atgagtatgc tataaacaac
    42481 atacttcttc atttaatgat aactatagac agaataaaaa agaataatta catagactct
    42541 gttgaagtag taaactacat agacaataac gtagatataa atattgcaaa agacatatca
    42601 aattttttag aatctgaata caatataact ttaactagtg cggaattata ctatttagta
    42661 tttcagttaa caaacaaaac tacagtctta aactacaatc aaatggatac taaatcattg
    42721 tcaaattata tagatgaaca ctttgttaaa ttaactaaaa aaataattaa aaatgtatat
    42781 gacttatatc ttatagattt atctgatgag gaatttgttg taaaatttac tcttcatgta
    42841 aaaaatttaa tcagtagagc caaaaataat caagttttaa gaaatcaaat acctcagaag
    42901 ttaaaagatt cctaccctct gatatatgat atatctgttt atatttgtaa tcagattcaa
    42961 attttagaaa acatagaaat agatgaagat gaaatatcgt atatatctct tcatgtaggt
    43021 tcattttttg atagacaaaa attactagaa gataaaattc tctgtgcatt aattactcca
    43081 aattactatg atttacaatt caaaatagta agagaccttg agaagagatt taatgaatcc
    43141 atagaaatta ttcagatttt ttcagatact cataatcttg attttgacaa taaggtagac
    43201 atggttatta ctactttacc aattaacaat agatgcccta taccttttgt ttatgtaaat
    43261 ccttacctaa atagaaagga ttatgataat attcaaagta aatttattca gattaaagat
    43321 aaaaaaaata tacttactgt acaaaatcat ttagaaatgt actttagtga atctttgttt
    43381 atgaaaaata tatatttaga tagtgctaaa gattatataa aatttatggg aaatattctg
    43441 tatgaaaata aatatgtcaa acccaattat atagatgatg ttcttataag agaaaaaatg
    43501 tcttcaactg catttaataa caatgtagca attcctcata gcatgaaaat ggatgcttta
    43561 aaaactggag tatgtttaat tgttaacgat aaaccagtta aatggggtga ggaaaaagtt
    43621 caaattatag ctatgatacc tataaatgaa aaagaaaaag aaaagttcaa ttacatattt
    43681 gaaagcttta ttgaaatatt atctgagtgg aataatgtta aagaacttac taaggcagat
    43741 aactatagta gttttatgaa tagaatagct tatcttattc aaaatatcta aatccgttaa
    43801 tttatttcca atatatgttt cttaagttat taacataaaa tagataagct gtcacactaa
    43861 aaagtctaaa ttaatatgtg acagcttttt acctaatata ccttgtttta atactacata
    43921 ttttttgact gttgtatact agttataaga ttgaatattt gtgttgttgt agctgtcata
    43981 ttctcactag cagttgcttt atccattgct tcttctattg tcatagctct atttactata
    44041 gaaaaataag catctatacc ctcttcatta catttaactg catcttctgt tgtggctcca
    44101 gccaaaccta ttacttttac ctcatatttt tttgccagtt ttgcaactcc tattggagcc
    44161 ttgcccatag cagtttgatt gtccaatcta ccttcaccag ttatgacaaa atctgcatct
    44221 tttaactctt cctctaattt aatttcttct aatattattt ttattccaga ttctaatttt
    44281 gaatttaaaa atgctaaaaa agcaaaacct aatcctcctg ctgctcctgc tccttctaca
    44341 ttagctacat ctttacttaa gtaattttta actactttgg aaaaatttct aagcccatta
    44401 tctaactcct caattatttc tgatgttgca cctttttgag acccgtatat gtatgcagct
    44461 ccatttttcc caaacagcgg attatttaca tcacaggcta ttttaaactt acattcatct
    44521 aattccttta atctattttc tatttttata tgtcttattt catttaaaac ttttccacct
    44581 aatcctacta acttgttatt tttatcataa aactcaaacc ctaaggcttg aagcatacct
    44641 atcccagcat cattagtagc acttccacct atcccaacta taaaatttct atagccttta
    44701 tttattgctt cctttattat ttccccaaca ccataagttg tagtgtataa aggatttcta
    44761 agttcagtcg gaaccaatgt taatcctgat gcttgagcta tttctattat tgctgtactt
    44821 gtatttttta ataatccata tgttgcatcc acttttttag ctattggtcc agtaacttct
    44881 atagtctctt ttttaccatt catcccttga actaaagcat ctactgtccc ttcaccacca
    44941 tcagcaagtg gcaatatctt tacttgcgca tcttctttaa ccttcaatat cccctttttt
    45001 attgcatttc ctgcctctat tgaagataag cttcctttca aagaatctat agaaattaat
    45061 attttcatac ttatctactc ctttttatta ctccttattt taaacatata attttataag
    45121 cttaatttat cttttatttt atactctaca tctctctttg tatacaactt ataccattaa
    45181 tattctttta tcttataaaa agggagtgtc tcaaaataaa tttcttagtt catgagacac
    45241 tcttttgtat aaaatcaaat atatgtttat tatttcaaca atgataagga ggattatctc
    45301 tattttaaga taattccttt ttagcactat ctaatcattt ttagcactat ttaaccagta
    45361 tagttttaac ttggttcatt aagcactcta catttacaga gttattatct attgatatag
    45421 attctaattc tttttcattt aggttaatcc atttagctgt tttaatattt ttgttaaata
    45481 ctatgttagc tgattttgtt tcttctttag aattataaaa tctaactact aatgcctctt
    45541 ctttttcagc cttttttaca acacttaaca caagatcctt attagtttcc ttaagtaaac
    45601 tatatgaata atctacttct atttctgatt gattcaattt catagcatta tatggcattt
    45661 tattataagt ttgtattttt gttaaatact ctttagccat atttgcaaca tttgcttcta
    45721 gtgtagattt ttcatgagta actattgcta agtctatttt tatatctcct atcatttgag
    45781 aatctggagt aggcaacttt attcctgatg gacgacctgg gcgtcttgcc atttcttctt
    45841 tacctaggaa tcctacactt ctaaataatg ttatagctat tgtgtcaaat tttttcccta
    45901 ctatttcaaa ctctcttgta ctgtttgtaa gaacagctac tccatgctct ttgtcagaca
    45961 atccaacgaa tgttagcatt ggatatatag aatctggtct ttcactccag tcttcctgtt
    46021 gccaaacttc catagcatca tcatacacac ttctctttat atgtccaaac tgattatcag
    46081 acacagaaaa ctttgaagct atatttgttg gtatataagt tctaacccta caatctcttg
    46141 cttggttatt tatgttgaat actacatcta ttatagcttt atcatttgga acatttataa
    46201 ctatatcaaa gtcaataaat gaatctgctc tattattttt tctagcttgt atatcttttg
    46261 gtacttttaa tctataattt atctttatct cattattaaa tctatttttc tttatagtgg
    46321 aatttgctac tatattatcg ttaaatatta atttctcatt tggaagtggt gaaaaatcat
    46381 actcatctcc ttcatctcct ccattttcca tcaataatac attttcaaaa gttttattta
    46441 attttttatc aaatatcttt aatgtcccgt tttcattaac ttgaatagta taaaagtcat
    46501 tatctaccat gtcacatact tcataagctt tttctaacat atgtccaatt tcttttatca
    46561 tatacgcctt ataccccatt gcaggtattt tatctttaaa gtttattgta tataccataa
    46621 atgaatcata gtttccataa tgaacaattt gacggtctat taatcctgca tctattactt
    46681 ctttatgtac cacttcaaaa tccaacttat tattatcttt atcaaatatt tcaaatgctt
    46741 tcattttagt tataacttgg gcgcttacta tttcattcct atcgtaaggc attaagttaa
    46801 atgctactaa cttatctaaa gccatttcac aactcattgc atcaactatt cttcttttgt
    46861 aaaatgttat taattggtct actttttcct cagctaagaa aaatctattc attatttcat
    46921 ggtgaacttt atctgaacaa caacatccaa tgctgtcatg agcatgattt ttcattattt
    46981 ctttccaaat taactctatt aatccatgat gatattcaaa tcctagagaa tatgcaatag
    47041 aagctagtgg ctccaatatg ttggttatct ttgattctat tctagcattt gctgatttta
    47101 tatccattct tgttgagaat atacttctat gaaccctcat atatttccca tctaaaaatt
    47161 ccccttttat tgtatctata tcctcatttt tttctagctc tttaaatatg ttttcatatc
    47221 tacttaagaa aaattttctc tctggataac attcttttaa tttatatata acttcaaata
    47281 tattcttttg aattggcatt tggtcatgtc catttggaag tattatatgg tcagtagttg
    47341 cacccttatc aagaaccggt aaatatttat ccattcttgt tcttagctca tcttcattca
    47401 ttggtagata ttttcctatt gcataaccta atggaagtat ttgaaccaaa acagaagact
    47461 catcatcact cttccaatta aactctgttt tattagttcc ttttctttca gaacatcctc
    47521 tccaaaacat tgagtatttt atatcaaacc catttagtat ttgtggcatt tgagcacttt
    47581 gaccaaatga atctggtaaa tatcctattt tcatatattc tccaaattca tcacaatctt
    47641 ttattccata tagtaaattt ctaagtatgg attctccacc aactaccatt tcatcagttt
    47701 gagtatacca gggacctatt attagccttc cttcttgaac taattttttt attctctcct
    47761 tacattcagg cttaactgct aaataatctt ctaatatagc agtttgacca tccatcacat
    47821 aatatggata gttatgatta ttttctaaca tatccattat ttcttccata ttatttacaa
    47881 gtaatattct agattcctca gttgagaagt accattctct atcccaatgc atatgaggaa
    47941 ctatgtgtac attttttttc attgcttatc ctccaaaact tatctgataa tattttctgt
    48001 ctaattaaaa atccaataaa aatactactg agtttacaaa attttttaat atttatttta
    48061 tcactataat taaacctttg atttaaatat ctaataacgt atcttttaat gtatctttct
    48121 ttgatttttt tgtttcctag ttattattaa taatattgct gatattatag ctccaagtac
    48181 tgccgcaaag aaccatattg atgctataat aaatcctgac atacttccct ctaaaagcgc
    48241 caatgagaat atccccgctc caggcacttg aagacctata ttgaagaatg ctactataga
    48301 cccagttatg gctgaaccag ctaccaatga tggtataaca cggattgggt cctctatcat
    48361 aaatggtatt gcaccttcag ttattccagc caatcctaat atccaagttt gctttcctat
    48421 ttccttttct gattctgaaa ataaatctct gcctaaaaca gtagctattg taactccaaa
    48481 agctgaaacc atcttaactg atgcaaatat tgcataaggt ataaaatttc cacttgctat
    48541 tgctcctatt gcaaatgtat aggctgcttt atttattggt ccacctaagt caaatgaaac
    48601 catagctcct attattgcac ctagtagtat tgcatttact ccagtcatcc catttaacca
    48661 atttataaga ccattgttaa gtgctgctac aggctttcct actacaaata acatcaataa
    48721 acctactacc aaagaaccaa ctactggata tacccaaaaa cttacgaatc cagctaatgt
    48781 gcctttggct ggtatattct tctttaaaaa ttttactata taaccagcaa gtaaacctcc
    48841 taacatacct ccaagaaatc cactacctat taaatcagct gctagacctg ctgcaaatcc
    48901 tggtccaaga cctggttttt cagctagtga gtaagacata tatgctgata atactggaac
    48961 catcaatgta cctaacatac ctccagaaag ctttcttaat tgccataacc aagaaccttc
    49021 ttggttgtat agttcttgta gaccaaatgc ttgagatgct aatactgcaa atgcaagtat
    49081 catacctcct gccacaataa gtggtattat atgagatact cctgtaagta ttgctgattt
    49141 tacatctttt ttccatgatg atttttcaca atatgatata tcagatgatt tttctatata
    49201 atcaccttta ccttgttttt gtgctacttc taaagcttcg tatattattt cttttgctct
    49261 ttttagtgga gctgctactg acgtttttat gtttggcaaa tgttcaaatc tttctgtatt
    49321 tttaggtgct acatctgttg catatattat tgcatctgcc ctttttatta tatctggtgt
    49381 atgagggtcc accataccat tagctccttg cttttctaca tacacatcca cacctatttc
    49441 tttaccagct ttaattaatg cttctgctga catatatgta tgtgctatac cggcagggca
    49501 tgctgttatt gctaatataa ttttttttga ttcattttga atcctaatct cttcttttat
    49561 ttgttctttc ttttctttta tatctaagtt attaaataac tctttcttag tttttgaatt
    49621 taacaacata ttcatataat cttcatttga cagtctagta actaactcag aaagtatttg
    49681 tatgtgtgta tttccacctt cactttctgg tactgctatt aaaaatatta gctctacttt
    49741 attatttggg tctacgcttt cccaattttc tattggtgtc tctaaggtca tagctgcaaa
    49801 tgcagcttct ttaactgact tagattttcc atgaggaatt gcaagccctc tttcaaaacc
    49861 tgttggagat aaagtttctc tgtacattac agcttctaaa tattcctcat aagatgttaa
    49921 cttgccttca tcatatagct ttcttgctaa gaactttatt gcgtcttcct ttgtttttat
    49981 tttttgtttt aaaaaaacaa ggttttcatt tgtgattttc ttaaattcca aaattacgcc
    50041 ctccttaaat catttaatgc tatgaatata gcataaaata aattatgaaa atgttttttt
    50101 gaaattatac acacaatttc ttcttaaaaa ataaagattt gtgtatttat acacaaatct
    50161 ctatcttaac caatattgct ctattattgc tctcataatc atcataattg gaaccttatc
    50221 tgatatattg tacccattta cttgtttaat tgttgaatag caatataaac ttatatctgc
    50281 aatttgttgc aattgatttt cacatatatg tgttaaagct actaccttac aacccttatt
    50341 aactgctgtc tttgctgctt ctataacttc tttcgtttct ccacttaaac taagtgcaaa
    50401 aaacatatct tgttcaccta attttcctat atcatgaagt acttcatgtc tttgactata
    50461 gtaagttata ttatcatgcc cacctctaat ttcttttact aatatctcac ataaaggaat
    50521 gttttgacct attccataaa ctataatact cctagatttt tgaattatat ctgctacttt
    50581 atttattttt tcataatcta ttagctctaa tgtctttttt atatctgatt caaaaccatt
    50641 ttttggctct ttatcctcat tttttaactc ttcttttaaa ttatttttta gttgagagaa
    50701 accatcatac tccatttttt tacatagcct aactatggta tttgggacag taaaaaattt
    50761 atctgctaaa gattgtattg ataattttac tatttcttct ttgttttttc tcatatattc
    50821 aattatcatg tcttctgtat ctgtaaattt atactcatat ttttttgctc tttcataaaa
    50881 ttccatagtt tctcctccat ctattttatt attcataagt aaaattcaaa aatattagct
    50941 aaaattttcg ttaatataat tataatttaa t
//
LOCUS       DAELJO010000008        44033 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELJO010000008 DAELJO010000000
VERSION     DAELJO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.008"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            132..482
                     /locus_tag="KPB88_000404"
     CDS             132..482
                     /locus_tag="KPB88_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0052714.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            501..2144
                     /locus_tag="KPB88_000405"
     CDS             501..2144
                     /locus_tag="KPB88_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG0052715.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLNML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2225..4282
                     /gene="recG"
                     /locus_tag="KPB88_000406"
     CDS             2225..4282
                     /gene="recG"
                     /locus_tag="KPB88_000406"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG0052716.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4341..4895
                     /gene="rsmD"
                     /locus_tag="KPB88_000407"
     CDS             4341..4895
                     /gene="rsmD"
                     /locus_tag="KPB88_000407"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG0052717.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4897..5394
                     /gene="coaD"
                     /locus_tag="KPB88_000408"
     CDS             4897..5394
                     /gene="coaD"
                     /locus_tag="KPB88_000408"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG0052718.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5398..5922
                     /locus_tag="KPB88_000409"
     CDS             5398..5922
                     /locus_tag="KPB88_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052719.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6266..8185
                     /locus_tag="KPB88_000410"
     CDS             6266..8185
                     /locus_tag="KPB88_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0052720.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8198..8485
                     /locus_tag="KPB88_000411"
     CDS             8198..8485
                     /locus_tag="KPB88_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0052721.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVEVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8618..9877
                     /locus_tag="KPB88_000412"
     CDS             8618..9877
                     /locus_tag="KPB88_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG0052722.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9880..10620
                     /locus_tag="KPB88_000413"
     CDS             9880..10620
                     /locus_tag="KPB88_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0052723.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVGPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10961..12253
                     /locus_tag="KPB88_000414"
     CDS             10961..12253
                     /locus_tag="KPB88_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0052724.1"
                     /translation="MEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSKKDGEKE
                     KLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDV
                     GYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLY
                     YNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNW
                     YPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFG
                     PGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKEL
                     AYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPLVVGPHG
                     VEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12351..13268
                     /locus_tag="KPB88_000415"
     CDS             12351..13268
                     /locus_tag="KPB88_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0052725.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13284..14093
                     /locus_tag="KPB88_000416"
     CDS             13284..14093
                     /locus_tag="KPB88_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0052726.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14151..15899
                     /locus_tag="KPB88_000417"
     CDS             14151..15899
                     /locus_tag="KPB88_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG0052727.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16161..17183
                     /locus_tag="KPB88_000418"
     CDS             16161..17183
                     /locus_tag="KPB88_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG0052728.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17188..19494
                     /locus_tag="KPB88_000419"
     CDS             17188..19494
                     /locus_tag="KPB88_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0052729.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19466..20581
                     /locus_tag="KPB88_000420"
     CDS             19466..20581
                     /locus_tag="KPB88_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052730.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20599..22017
                     /locus_tag="KPB88_000421"
     CDS             20599..22017
                     /locus_tag="KPB88_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0052731.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYLFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22032..23240
                     /locus_tag="KPB88_000422"
     CDS             22032..23240
                     /locus_tag="KPB88_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052732.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMNGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23321..24709)
                     /locus_tag="KPB88_000423"
     CDS             complement(23321..24709)
                     /locus_tag="KPB88_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0052733.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25050..26384
                     /locus_tag="KPB88_000424"
     CDS             25050..26384
                     /locus_tag="KPB88_000424"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG0052734.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26513..27430)
                     /locus_tag="KPB88_000425"
     CDS             complement(26513..27430)
                     /locus_tag="KPB88_000425"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG0052735.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27638..28522
                     /locus_tag="KPB88_000426"
     CDS             27638..28522
                     /locus_tag="KPB88_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG0052736.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28735..30576
                     /locus_tag="KPB88_000427"
     CDS             28735..30576
                     /locus_tag="KPB88_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0052737.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGEHTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30881..31588
                     /locus_tag="KPB88_000428"
     CDS             30881..31588
                     /locus_tag="KPB88_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0052738.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31582..32892
                     /locus_tag="KPB88_000429"
     CDS             31582..32892
                     /locus_tag="KPB88_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0052739.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33009..33692
                     /locus_tag="KPB88_000430"
     CDS             33009..33692
                     /locus_tag="KPB88_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052740.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33685..36072
                     /locus_tag="KPB88_000431"
     CDS             33685..36072
                     /locus_tag="KPB88_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0052741.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYALSNIIIK
                     SVMDIDVKSSQLPVILLVIIISFMSVVLSLLKPMKVASKVSIVDAVRYTGNKISNKNK
                     RKGYKNINLKRLSQANLERNKKRTYMTLVSLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFIITYLIGRSFNKESLIDRVRYSE"
     gene            36352..37494
                     /locus_tag="KPB88_000432"
     CDS             36352..37494
                     /locus_tag="KPB88_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0052742.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDVDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37513..38037
                     /locus_tag="KPB88_000433"
     CDS             37513..38037
                     /locus_tag="KPB88_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0052743.1"
                     /translation="MEVTAKQKLTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLIGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38137..38955
                     /locus_tag="KPB88_000434"
     CDS             38137..38955
                     /locus_tag="KPB88_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0052744.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPIQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39033..39344
                     /locus_tag="KPB88_000435"
     CDS             39033..39344
                     /locus_tag="KPB88_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG0052745.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39521..40204)
                     /locus_tag="KPB88_000436"
     CDS             complement(39521..40204)
                     /locus_tag="KPB88_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052746.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40297..40938)
                     /locus_tag="KPB88_000437"
     CDS             complement(40297..40938)
                     /locus_tag="KPB88_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0052747.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41112..43115)
                     /locus_tag="KPB88_000438"
     CDS             complement(41112..43115)
                     /locus_tag="KPB88_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0052748.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43268..43906)
                     /gene="recX"
                     /locus_tag="KPB88_000439"
     CDS             complement(43268..43906)
                     /gene="recX"
                     /locus_tag="KPB88_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG0052749.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17139 a         4824 c         7564 g        14506 t
ORIGIN      
        1 acttttctag gtttaaatta taccatattt atagtatgat atgattataa ttttatataa
       61 taaataagta aaaatagtaa aaaataaatt aagaaatgtt tttaataaaa ataagcttaa
      121 gggggtcaac catgagtgca aaggtaacga atcaatatgg aaatatagaa atagataatc
      181 aagtaatagc tcaagtcgta tatagagctg caatggaaag ttatggctta gtaggtttag
      241 catataagtc taaaggaata gtagagctat taaaaggcga gaatgcaact aaaggtgtaa
      301 gagttgagga agtagaagaa gatgccatag caatcgaact ttatgttata atacaatatg
      361 gtacaaatat atctactgtt gccaataata taatagatag agtaaaatat gtagttgaaa
      421 aaatgactgg cgtaagagtt actagaattg atattaatgt acaaggaata agagttaagt
      481 aatttcatag gaggaaacaa atgattcaat atatagatgg taaaaggtta agggagatgt
      541 ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt agttgacaag ttaaatgtat
      601 tccctgtacc agatggagat acaggaacta atatgtcatt aacaatatct tatgccttaa
      661 aagaattagc aaaagttgaa aatgataata tatcagatat cggaaaagca ttatctaaag
      721 gttctttaat gggagctaga ggaaactcag gagttatact ttcacaaata atcagaggta
      781 tagctaaatc tatagaagga aaaagtaaat tatcaacaga agatttagca aaagctttta
      841 aaaatggttc agacacagca tataaagctg taataaaacc aattgaagga actatactta
      901 ctgtagttag agaaagtgga gagtttgcaa taaaaactgc gaaaaaagag aaagatgtag
      961 taaagttttt aaatatgctt gtgaaggaat caaattcttc attagaaaga actccagacc
     1021 ttctaaagaa tctaaaagaa gctggtgtgg ttgactctgg tggaaagggt cttgtactta
     1081 tatatgaagg aatgttagct tctataaaag gcgataacat agaaataaaa aatgttgacc
     1141 tagatactaa tatttcaaca agtatggatt ttacaaagag tactactagc acagatgata
     1201 taaaatattg ctattgtaca gaatttattt tagaaagttc taaggtagaa gatactaaaa
     1261 taagagacat aatgatggct tatggtgata gcctagcagt agttggagat gatggagtca
     1321 taaaggtaca tgtacacact aatgacccag gaaatgtact tcaagaagct ctaaagtacg
     1381 gtcaattatt aactataaaa atagaaaata tgaagttaca acatgaaaat acattactgg
     1441 atgtagaaga aaagaaagaa aatgatagtg aacctttaga agaagaaaaa gagtttgggt
     1501 ttatagctac ttctatgggt gaaggtttgg caaatatatt taaagatttc ggagttgacc
     1561 atataataga aggtggtcaa actatgaatc caagtactga agattttatg aatgctataa
     1621 agcatataaa tgctaaaaat atatttattt tcccaaataa cagcaatata ataatggctg
     1681 ctaatcaggc aaaagaattg agtgataaaa atataatagt tatacctaca aaaaatacgc
     1741 ctcaaggatt tgctgcatta gtaactttta atggagaatt aagtgaagat gaaaataaag
     1801 aagctatgat gaatgcttta aactcagtta aatcaggtca agtaactttt gctgttaggg
     1861 atacagtgat gaacgaaatt gatgttaaag aaggtaatat aattggaatt gcagaaggga
     1921 atttattatc agcaggagat tatgtggatg aagtaacatc aaaccttatt gaaaagttgg
     1981 ttgatgaaga tactgctata ataacattat tctttggtga agatgttaca gaatctcaag
     2041 caaatgaact acgtacttct ttagaagaaa agtttgaaga tgtagatgta gagttatatt
     2101 atggaggaca acctctttat tattacttaa tttcagttga ataatgatta taagaacctg
     2161 tatgaccaag tagcttttat aaaattggtt gtacaggatt tttgtgtagt aggtgatata
     2221 tttgttggat ttatataaag atgttcaata tgttaaggga ataggaccta aaaaagcaga
     2281 taaattaaat aaactgggta tatttacact aaaagaccta ttgtattatt ttccaagaca
     2341 atttgaagat agaaataatc taaagaaaat agcacaatta gaagatggtg agaaagttac
     2401 gataaaagca gtaatatcaa gtataaatac atttagccca aaagaaggta tgactttaac
     2461 aaagattgat gtaaaagatg agacaggctc ggctaaatta gtgtttttta ataagagtta
     2521 tataaaaaat acttttcgtc caggagattc aatattagtt tttgggaagg ttaagaaaaa
     2581 atttaataac ttagaactta catcatgtga attagaatat cttactaatt cacctaagaa
     2641 tacatgtaga tttatgcctg tgtaccagct tacatacggt gttacaaata aagaaattat
     2701 gagtataatt agaacagtac ttgaagataa agaattaatt atacaagaat atatgccaca
     2761 aagaattata gaaaaatata ggctatgtag tatagatttt gcagttagaa acatacattc
     2821 accaagtagt aaggaatcat taaaaatagc tttatataga attgtatttg aagaattact
     2881 tatactacaa ctagggttgt ttgtatttaa aagtggcaga aataaagaag atggaataaa
     2941 gtttgaaaca agtaaagact taaaaaaaat aataagtgct ttaccattta aactgactaa
     3001 agctcaaaac agggcattag atgaaattat acaagatatg aatttagaga aaataatgaa
     3061 tagattggta caaggtgatg tagggagtgg taaaacagta gtagctctct tagcacttgc
     3121 aaattgtgtt ttaaatggtt atcagggcgc tttgatggca cctacagaga tattagcagg
     3181 gcaacattat atttcactta ctgagtctct aaaagatttt ggaataaatg taggtctatt
     3241 aataggtagt cttacaaaaa aacaaaagga tacagtattg gaacaaataa agaataatga
     3301 gattgatata ttaataggta cacatgcact aattgaagat aaggttgaat ttaataatat
     3361 tggtttagtt ataacagatg aacaacatag atttggtgtt atgcaaagga gcaaactttc
     3421 attgaaagga gcaaatcctg atatattagt aatgacagca acaccaatac caagaacttt
     3481 agcacttata ctctatggag atttagatat ttctattata gatgaacttc ctccaggaag
     3541 acagccaata gaaacaattg ctattgaaaa aagtaagcga gatagagcat ataataatct
     3601 tgtaagaaga gaggttgaat caggaagaca agtgtacatt gtatgtccct tggtagaaga
     3661 aagtgaagcc atagaagcca aatcagcagt tgaacttgtt gaggagttaa gagcggaata
     3721 ttttcacgac ttaaggcttg ggttgcttca tggtaaaatg aagtcaagtg aaaaagatga
     3781 agtaatgaga ctttttaaag ataaagaaat agatatttta gtttcaacaa cagtaataga
     3841 agttggtgtc aatgtgccaa atgcaacatt aatgataata gaaaatgcag aaagatttgg
     3901 acttgcacaa ttacatcagc ttagagggcg agttggaaga ggtagtcata aatcgtattg
     3961 tgtactaatt tatgattcta aaacagatgt atgtagacaa agaatggcta taatggaaga
     4021 gaccaatgat ggttttaaga tttcagagaa ggatttagaa ataagaggac ctggggaatt
     4081 ttttgggaca aggcagcatg gtcttccaga acttaaggta gcaaacttat ttaagcatat
     4141 aaaaatactg aagttagcac agcaagaagc tcgatatata ttgggtgaag ataataatct
     4201 acaattaaaa gaaaacatgg cactcaaaaa agaaattata gataaattta aagatacatt
     4261 gaaagaaatt tcattaaatt aattttaaat tgtgttaaaa tattactaat gtaataatgt
     4321 gctgaaggga gattgtcata ttgagagtaa tttcaggaaa agcgagagga ttaaaactaa
     4381 atactccaaa aaatgaagat gtaaggccaa ctacagatag agtaaaagag tctctattta
     4441 atatgataaa ttcatatata atggaaagtg aagttctgga tttatttgct gggactggtt
     4501 ctttaggaat agagtgctta tctaggggtg ccaagtcatg tacttttgta gatatcagta
     4561 aggaaagcat agatattgtt aagtccaata taaaaaaggc aagagttgaa agtgaaagta
     4621 ttattttaaa ccttgatttt aagactgcaa tagataaact taaattacaa aatagtaaat
     4681 ttgacataat ttttatggac cctccgtatt ataaaaatat gtttatagaa gctattgaaa
     4741 aaattgataa ttctaatcta ttgaacgaag atggaataat agttgtagaa catgatacga
     4801 atgatttatt tccagataag atatgtaaat tggagaaaac tagagataag aaatatggaa
     4861 atactacatt aactttttat aagatggagg cataatatgg aaaataaacc tagaaaagct
     4921 atatttgcag gaagttttga cccaattact aatggacatt tagatattat ttgcagggct
     4981 tcgaagttat ttgacgaatt acaaatagga gtattaaata atcctaataa aaagggtcta
     5041 tttagctttg atgaaagagt aaaacttata gaaaaaagta caagccattt aaataatata
     5101 aaagttgtga cttttgatgg tttattaata aattactgtc aggaaaatgg gattggagca
     5161 ttagttagag gtgtgagaag tggtgctgat gttgattatg aacttcagat ggcgcatatg
     5221 aatagagagc ttaatccaga tatagagact ataattttac caagttgtac aaaatattca
     5281 tttataagtt catctttaat aaaagaggtt ttgctctttg atgctgatat aaaaaatttg
     5341 gttccaaaga ttgttttgga agaattaaag aaaaaaacta gtgggggtaa ttgatatatg
     5401 aagatagatt tagaaatgat tgaactattt gagcagttag aggagatatt tagaagtgct
     5461 tctacaatcc ctttctcaca taaatgcaca gtggataaag atgaggtttt agcaataatt
     5521 aatgatataa gaactttaat accagaagaa gtaacacagg cagtatggat taataaagaa
     5581 agaaataaaa taataagtca agctaagcaa gatgctacaa atatagttga acaagcgaaa
     5641 aaagaagctg agagaataca acaagaatat caagataata tagaggaatt aaagaaaaat
     5701 tctgaagatg tagtaaaggc atatgtagaa tccagtgaac ctgttgttca agcagaccaa
     5761 agagcaaaag atatagttga tagagctgag agaatagcca atgagataag aattgggtca
     5821 attgaatatg cagaggatgt gctttctagt gttgagtata atttaagaga aatattagaa
     5881 gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat aaattttaaa taaataaaaa
     5941 tggagctatt acacaaaaaa ttccaattta tgtgtgatag ctctctttta atatagcaat
     6001 tttttgttat ataattgtgc tttttagatt acttatatct tattttgata ttataatgta
     6061 gtttaatctt ttatttgttg aaatcaactt atttatttgt ataaataata taggttattt
     6121 tcaaattttt ttaataattt taccactgtc ttcttttaga ttagagttag actgtgcaaa
     6181 attataaaat aatactaaat catgtgcaat tatttggtat ggcactacat gtagaaaata
     6241 tagataataa aattaaggag gtagtatgaa ggaaagggct aaaaaaatat tagattttat
     6301 tattaaaaat aacaatgtaa catcacaaga actacaagaa aagtttaatg tttcaaagag
     6361 aactatttat tacgatattc tagctataaa tgaacaacta ggaaaaagtg gtaatataaa
     6421 aaatgtaaaa cacaaattta tatttgaagg taatttatgt gatgcaagga aaataatttc
     6481 aactgaagaa gataaatttt tagatagtga ctacagaaaa acttttatat taaacaagat
     6541 attacttggc gaaaaaatat caatagaaaa actaacaaat gaaatgttac tttcaaaaaa
     6601 cacagtagta caaacaataa ctgatgtaaa aaaatacctt caaacaatgg ggttaagatt
     6661 agagtataag ggaaagtaca aaattatagg tgatgaatac gttattagag agctgtttct
     6721 gataattgtt caagaaaatg tactggaaat taattctata agtgaggaag taagtagttt
     6781 cgatacaaaa ggacatataa aacttactga ttattcttta ttaaatctaa ctaagtttgt
     6841 ggaatttcta aataaaagga taagagatgg gaaaacatta tactcatata aatatctaaa
     6901 tgaagctaaa aaaattagtt actttagtaa ttgtaaagaa cttttatgtg aagaagctaa
     6961 tgaaaatgaa caagcctata tatgcactta cataagctca cttcccagct taaatagtga
     7021 agttaaggaa gatgttgttg aagaatatgt agataaactt atagataagt ttgaagtaaa
     7081 tacagcaata aaattagaaa gtaagcatga gtttaagaaa aatattttaa ggcacctcca
     7141 ctcttcttac aacagaatac gatttaagtt tcctattaga aatccaatgt tagatgagac
     7201 taagtataaa catgagtcac tttataaaat tataaaatca attatagaga atgaagaaga
     7261 gtttcctgta tttgagggga taagagaaga agaaatagga tttatagcag cgtattttgg
     7321 aggatattta agagggtcta gggataatgg tctaagaaga aataaagtct tactagtttg
     7381 tcctaatggt cttatggtat caaagagttt ggaaatacaa ctatataaat acataccaac
     7441 catagaaata gtaggtatag tatccattaa gcaactaaaa gaagtaaatg tatactacga
     7501 ttacataatt acaactatag atatacaaaa tgtaaacaac gtaattgttg taaatcctct
     7561 tttaacatca tcagatgttc aattacttat gaacaaacta ataagtgtaa aagaaaacga
     7621 gaagtatttc aatcttgaat taataataca agctataaga aaaaatggag ttatcaataa
     7681 tgaagaagca ctaaaagctg atttattaaa cataatacac aagatagatg aaggagagat
     7741 gtatcaacca atgttaaagg aattaattaa tgcagaaagg gtcaatataa taaaaaatgt
     7801 tagagattgg aaagaagcaa taaaaatagc ctcaaagcca ttgttagaag ataactctat
     7861 agaagagtta tatatagaaa atatgataaa gtcagttgag aaatatggac catatattgt
     7921 tttagctgat agatttgctc taccacatgc aagttctaaa gaaggagtaa acaagcttgc
     7981 tatgtcttta ctaatagtag aagatgaagt tgatttatta ggaaaaccag taaatatatt
     8041 tatggtatta gctgctgtag ataacactac tcacattaga gcattagcat cactatcaga
     8101 gatgatgtac gaggaagaaa atgtaaaact aataattaat ggtgataaaa gttcaatact
     8161 agaacttata aataagcaaa attaggagga atgaaaaatg aagatattag cagtatgtgg
     8221 ttttggtgta ggttcatcaa tggtattaaa aatgacaata caaaaggctc taaagcagtt
     8281 aggtatagaa tgtgaagtag aaaatacaga tataaattca gctagaggtg ctgattgtga
     8341 ctgtatattc acaagtcaag aattgttaaa tgaattaaaa ggaacttgta atgtagaggt
     8401 atatccagtt aaaaagtaca tggatttagc agaagtcaca gaagttgttc aaactttttt
     8461 aagtgataaa aatgataaga aatagtataa gttttaaaaa gtaaatttaa gttaatcaga
     8521 ataaaataaa aacttactaa attcaaaaat agtaagaaag agaggaggga tttagtggta
     8581 gaaaacttaa acagttgacc tatcactaaa aaatataatg cttgatttta tttcaaataa
     8641 tattttacgt aatccatcta tgttaattgg attaatagca gcatttggtc ttgtactaca
     8701 gaagaagaaa gcatctgatg ttataaaggg gtcactactt gcagctttcg gaattataat
     8761 tatggagact ggaacaggta tgttagttag ttcaatagca ccaattaatg gagctttcca
     8821 aagtataagt ggtggtgcag taacaaaagg tttaagtgat gttactttta ctgcaagtta
     8881 tggtggaatt ataggtttag caatgtttgc gggtttagct attcacctaa tgatagcaag
     8941 atttactcct attaagacaa tcttcttaac aggtcacatg ctttattggt tcccattcat
     9001 atttgtagct tcaggagttg aagctggatt aaatggtgga gtattaatag cttttgcagc
     9061 agtattatca gcactctatt ggtcaataat gccttggtta ttaagaaaat atgtgtttga
     9121 tgttatagga gatgattctt ttacattagg tcatccatct ggttgtttag cacttatatc
     9181 aggatttgtt gctaaattag taggaaataa agaaaaatct acagaagata taaacatacc
     9241 tcaaagttta tcatttttta gagaaatatc tataagtggt ggaatggcag tgtttttaat
     9301 gtttatagtt gttgggatag taataccagg tgcgtttaaa gatagtaatg agtttattgg
     9361 aacgtatgca ataaagcaag gtctttcttt tggagcaggt cttataataa tgcttcatgg
     9421 ggtaagaatg ctaataaatc aaataatgcc agcatttcaa ggtatatctg aaaaagttgt
     9481 gccaaatgca ctacctgctt ttgactgtcc aatattattt aattataaac caaatgctgt
     9541 attaatagga tttttagttg ctatgctaac atctacagct atgattttaa tatgtaacta
     9601 ctttaatgta tttaatttgc tacttatacc acttgttata acttcattct ttgagtgtgg
     9661 agcagcagca gtaataggag aatcacaagg tggtatacgt ggagctataa taggtacagc
     9721 tatagctgca atatttatgg tagttttagt aggaatatct gctgtaattt actcaggaac
     9781 tattcaaaat tggatactaa tatttggagg taatgacctt tcattatttg gtataatcag
     9841 taggtttata ggttcactat ttggaggaat attatagata tgaaatttaa atacatagat
     9901 gagataagaa gattaattga tataatagaa aacgaagaaa aagtcaatat ggaaaaagca
     9961 gtacaaatct ttgttgaagc aatagaaagt aaaaatgcta tattttcatt tggagcttca
    10021 catgcaggaa tattaacaga agaattattc tatagagcag gtggctgtgt tcttataaat
    10081 ccagtatttg caaaatctct aatgttagat acagcgccta taactcacac aagtaagatg
    10141 gaaagattag aaggatatgg tacagctcta gctgataaaa ctccaattaa aaaaggagat
    10201 gtagttatag ttcattcagt atctggtaga aatccagtaa gtatagagtt tgctatgaaa
    10261 gctaaggaaa ataaggcaat agtaatatgt ataactaatt taagttactc aaaaagtgta
    10321 tcttcaagac attcttcagg aaaaaaactt tatgaagtat gcgatttagt gatagataat
    10381 catggtcaaa agggagatgc ctgtgtagag atagatggat taaaccaaaa agttggtcca
    10441 acttctactg taatagcaac tactgtgatg aactctatta tagctcaagc tactcaggaa
    10501 ttagttaata aagggctaaa gaatcctccg attttctata gtgccaatat tgatggtgga
    10561 gatgagttaa ataagaagat ttttgatgaa tataaaagtg taattcatta tgaatattaa
    10621 ggttaaaaaa atatgttaat gaactttgaa gtttatcaac aaagaatatt aataatatat
    10681 aataaatgct atcatttaaa agctgtaggt aaaaatctac agctttttat tattgtagat
    10741 taaaaaggtg cgtaattaaa ttttaaattg gagaaaataa attggaaggt atcaattata
    10801 aatattagaa acgttgtcat agaaaaaaac tatattaaaa aaaagtacat agaaaaaact
    10861 gattagacaa taaatacatg ctttgataat attttaaatg gagtgaaaat gtgtagaatt
    10921 ataatgaaaa aagaaaataa aaataaaaaa agaaaataaa atggagggaa taaaagtggg
    10981 tatgttaaaa aaattatgtt cactagctat ggtagcaatt atgactacaa cacttataac
    11041 agggtgttca agtggaaatg gaaaagattc aaaaaaggat ggcgaaaaag aaaaactagt
    11101 tctttggatg ccaccagtag aaaataatat gaaggaagtt tgggacccta ttttagataa
    11161 atttgaggaa aaaaataact gtgaagtaga tttacagata attccttggg ataattattc
    11221 tgaaaaattt gcaacagcta taagtgctgg cgaaggtcca gatgtaggat atatgtatgc
    11281 agaaatgtat ccacagttta tagaaagtgg agcagtagaa gatttgacta attatgcaac
    11341 taaagaggat aaagagcaat ctatttatat aaaaacatct gaaatgatgg gtggaatgta
    11401 tggtatgcca ttccaagcag caaatccagg ggtattatac tataacaagg atattttaga
    11461 gaaattagga gaaaaacctc ctaaaacatg ggaagatttt aaaagaattt gtaaaaaagc
    11521 aacacaagat actgatggtg atggaaagat agaccaatgg ggattagctc agggatgggg
    11581 agctaaaact tttggtaact taaactggaa ctggtatcct tatttatggc aagctggtgg
    11641 agatatcttt aatgatgatt taaaatcggt taaatttaat gataagagtg gtttagaagc
    11701 agctaatttc ttaaaagaat tacaagcata tgtgcctgaa gattctttat ctaaagatag
    11761 taatgaaatg atagaaagtg tatttggacc agggaaagca gcatttacaa taatgttatc
    11821 ttctgctgca acaagtgtat ttgatgaatc attcccggat ttaaattggg gatttgtaac
    11881 tggactagaa gataagaaag cagcaacttt tggagcagta gaccatttat ctttaatgtc
    11941 atctgctaaa aataaagaac ttgcatacaa gttaatacaa catatgttaa gtgttgaatc
    12001 aatgacagaa tttcataaag caataccaag agcaccaata acaaaaggtg aaccatatca
    12061 aggtgatgaa agattcaaag aaatggtgga aaatgacaaa aatgtatata gaccattagt
    12121 agtaggacct catggagttg aaatatatga atatttatgg aaagaacttc aaactatgat
    12181 ttcaggagat aaaactccaa aacaagcgtt agatgatgct gcaaaatatt caaatgattt
    12241 attagcacaa taaaagttta aagctgtaat atgttgtaaa ggactttaca gattaaaatt
    12301 agcattgtat ttataaatac aatgctaata tttatattgg agaggataaa atggaaaact
    12361 tagttagaga gtttgaagaa aattcaaaaa gaaaaaaaac aaaattaaat attaaaccat
    12421 atatgtatat attgccattg ggaataattt tagtggcgtt ttatgtgata ccaataataa
    12481 tgtcaatata ctttagtttt acaaagtata atattattag tccagctact tttataggtt
    12541 tagaaaatta taaaaaatta tttacagatg aaacattaaa agtatctatt ataaatacta
    12601 ttaaatttac tgttgttgta gtaccttgcc aaactatatt atcattgatt ttggctgttt
    12661 ggattacagg aaaaggaaat agtaaaatag cttcatttgc taaaggtgct atatttatac
    12721 cagtattatc atctatggtt ttgataggta tggtatggag ggctttattg aatggagaag
    12781 gttctattat atatcaggta ttaggtgcct ttggaataga atcatctaaa cttttaggag
    12841 atagtaagac agcattacct acacttatgt ttatatctat gtggaaaaat atagggtatt
    12901 tcatggtaat ttatatttca gctataatga atttaccaaa acactgctat gaagtagcga
    12961 aggttgatgg ggctacaaaa cttcaagaat ttataaaaat aactgtacct ttattgaaac
    13021 caactacaat aatggttgta tttttagggt cgatatggtc attacaagta tttgacttaa
    13081 tttatactgt gacaggtgga ggtccaggta tatctactat gagtatagtt atgcatgcat
    13141 ttaacttaaa ctttaaaaac tttaattctg gatatgcaat gactgtagca aacgtattat
    13201 ttttgttaat agcagtagtt tcgattttac aaaataagct tgtaagacga gacaattcag
    13261 atttttagaa aggtaggtat aatatgaaaa cagcaagaag attattaagt gatggtttac
    13321 ttttatttat agcgtgtgca agtttggtac cttttatata catgttgata atatcattga
    13381 agataacata taactcatat agtttggata tatctttttc aacagtaacc cttcaaaact
    13441 atatagatat ttttactaaa aaaggatttg cacaatattt ttttaataca gcaatagtat
    13501 ctttttcagg tgtattatta aatttagtat ttagtacatt agcaggatat tcttttgcga
    13561 agatggattt taaaggtagt gataagttat ttttatttat gataatgact ttaattatac
    13621 cttcccaagt tacaatgata ccactatata taataatgaa acacttagga tggataaata
    13681 gttatcttgc acttattatg ccaataccaa ctgcatttgg cgtatttata atgagacaag
    13741 ctattttagg agtaccaaaa gagttattag aatcagcaaa gatagatggt tgctcggatt
    13801 ttagaatttt aatacaaata gtgttgcctt taattaaacc agcattaatt acactagcaa
    13861 tatttacttt tatgggagca tggaacgagt ttatgtggcc tcttatagct acaactaaag
    13921 atgcaatgag aacattaaca gttggattat ctactcttaa gacattccag ataactaatt
    13981 atggacagat gatggcaggg gctacaataa catttttacc tccatttatt ttctatttga
    14041 ttttacagag taaatttgtt gaaggtgtgt ctttgtctgg tataaaaggt taaatagtct
    14101 aatttaataa tatataaaga aacttatata aatatggagg aatttaaact atggaaattt
    14161 catatgtact gaaagattgc acattcaaac acaataaata ctcagttgta gaagctgaaa
    14221 cttggaaaga taaggatttg gatattgtag tagctaaagg tgaaaaattt gcatttcaaa
    14281 ttatgttaaa agcaacagaa gaatttaatt gtacaattga taaaagtagt gctatatcat
    14341 ggaaaggcct gggaaataga gtaaggttag cattaaatat gcctaatagt ttagaaaata
    14401 attttagtat aaatatatta ggatatgttc aagatgatac aaaggcattt gtgaatgatg
    14461 ctattttaag ggataaagat gtgttagtag agcaatattt gcctcaaaca ttttggattg
    14521 aaggtcaagt accagaagat tttgaagaaa ataaccttga tataggtatt gatatattta
    14581 aaagctttgg atatgaagac gaagaaaaag tttgtacaat tgatgttcct gtaaaagtaa
    14641 gaaatgttgt gctaaagcct ttagatgaaa gcaagttctt tttagattta tggcaacatc
    14701 caagctgttt agctagaatg tataaagttg agttatggtc tgatatacat tttgaaatag
    14761 tcgataacta tttaaaagaa ttagcatctt taggagaaaa agttgcaaca gttatagttt
    14821 cagattatcc atgggcaggg caaagttgtt ataaagttta taaaaatcca tcaaacttat
    14881 atgaatataa catggtaagt gtatcaaaag gattggatgg aaagattaaa tgtaactttg
    14941 agagtatgga taggtatata agcatagctg ataagtataa aatggctaag gagatagatt
    15001 tatttggtct attaggtaat tggtgtgctg gtgagtttgg aaatcctgta gaaggttata
    15061 aggaccctat aagagtaaga tattttgatg aaaaggataa agtatttaaa tttataaaca
    15121 atacaaatga tttaaaagaa tatataggat tagtattaaa tcatttaata gagtgtggat
    15181 tatgggacag agtaaggatt atagctgatg aaccaaataa tccagaagta gtcaaagaat
    15241 gtatagaatt tataaattct actgtaggta ctcatcaagt taaatataaa tcagcaactc
    15301 acgaccaaaa ctttttagac agagctaaag atgaaattga tgatatgtca ataaatttaa
    15361 aacttactat acaaaattat aaagatatag aaagtcttaa gaaaaaaatc aatgacaagg
    15421 gtgggatatt gacttggttt gtatgctgtt ttccagagaa accaaatagt tttttaagtt
    15481 ctccttttgt agaaaataga ataataggat ggtatactta ttactttggt cttgatggat
    15541 ttttaagatg ggactataat ttatggacag aagacccttg gaaagattca agttacaaat
    15601 tccctatttg gaaagctgga gatatgttct ttgtttatcc tggaaaagat ttaaagccag
    15661 ttagaagtgt cagaatggag aatttaagat ttggaataca ggactttgag ttatttacaa
    15721 tgttagaaaa agaaaaggga agagagtata tagaagttga gcttatgcaa gaattattaa
    15781 ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat agaactagga tattcacttg
    15841 ataattgggg atatgataaa gttaaaaaat atgtattaga gttgttagac agagtataaa
    15901 aaatataaga gtgtctcaaa atgaactttt tagttcatga ggcactcttt ttgtatgaaa
    15961 tcaatatagt ttcattgttt caacaatgaa aaataggatt atttctattc taagacagcc
    16021 ctttagtttt ttagttgtat tcaatgtttt aaaactaact ataaacgcta taaagtttat
    16081 aaatgacaat aattatatat aacatgatgt actttaattt gctataatag ggattgaata
    16141 atattatatg gaagataaca ttgtttgggg ggattaatat aaaaagaata ggtattatta
    16201 taatgttttt tttaagtata tcttttattg gattttatct tgtaataaga gataacagtg
    16261 atacagttaa gatgcaacga gggataaata taggtaatgc actagaatca cctaaggatt
    16321 ttccgtggga tgtaaaaatg tcaaataagt tttttgatga tataaaagat gcaggttttg
    16381 acacagtacg tataccagta aggttttctg attatacgtc agattctgat aattttaaaa
    16441 ttgatgaaga attttttaaa aagattgata aatatgtaga ttatgcattg gataaagact
    16501 taatagttgt gttggattta caccattttg aagaaattat gaaagaacca agagttcata
    16561 aagaaaagtt tttaaaaata tggcaacaga tagctaatag gtatcaaaaa tatgataaaa
    16621 aattggtatt tgaattatta aatgaaccta aagaaaattt atattcacag ttactaaacg
    16681 aatacattga agaagcaata aagataatta gaaaaactaa ccctaaaaga actattatag
    16741 taggaccata taatttctat caaattgatt atttgaatga attaaatatt cctaaagatt
    16801 ctaatatagt agtttccttt cactattacg agccaaatga ttttgcattt caaggaaata
    16861 tctatcataa gggatttgag cacttaagca atattacttg ggaaggaaca aatgaacaaa
    16921 tggattattt aaaaaaaagg tttgatactg tggaaaattg ggcaaataaa aataaggtaa
    16981 aaatcttctt gggtgaattt ggagtaacaa aagaagcacc agaaacttca aggagagctt
    17041 gggtaaaagc tgtaagagaa gaagctgaaa aacgaaactt ttcatgggct tattgggagc
    17101 ttgcttctgg atttggtata tacaatcaaa ttgaagggac ttgggataga gaaatattaa
    17161 gtgcattaat agaaaagagg tgattttatg aagaaaaatt caaaaaagat acttataact
    17221 ttaactatta ttactaatat agtatatata ttgtggagaa ttttttatac agtacctaaa
    17281 gaagaaggga tgtttgccct aatctgtgcc ataatacttc tttttgtaga gataatgggt
    17341 atgatggaaa tgtttgtgca ttattatggt atgtcaaata ttgagtaccc tgaaaaacca
    17401 atcataagtg aagaacttta tccacatgta gatgtattca ttgcaacata taatgaatct
    17461 gttgatttgg tgagaaaaac tgtaaatggg tgtattcata tgcagtatcc agataagaaa
    17521 aaggtacata tatacatatg tgatgatgga aatcgtgaag aaatgtgtat tctagcagaa
    17581 aaaatgggtg taaattacat aactagaact gaaagagaag gagcaaaagc tggaaattta
    17641 aataatgcaa tgcaacatac aaattcccct ttgattgcta catttgatgc agatatgata
    17701 ccaatgcatg actttttaat tgctacagta ccatactttt tgaaaaatga gcaagctaaa
    17761 aaagatggag aaaaagagga gtatgaaaag gttggttttg tgcaaactcc acagagtttt
    17821 tataatccag acttgtttca atttaatctg cactctgaag gaaggattcc aaatgagcaa
    17881 gattattttt acagagacat tcaattggct agaaatagga caaattctgt aatttatggt
    17941 ggtagtaata cagttatttc aagggaagca cttgaagaag ttgatggatt ttatacttat
    18001 tctattacag aagattttgc aacaggtatc ttaattcaaa gtaaggggta tagatgttat
    18061 gctattccag aagtacatgc atcaggttta tctccgacag atttaaaaag tcttattaag
    18121 caacgtgaaa gatgggcacg tggatgtata caaactggta gacgtctaaa tattttgttt
    18181 aggagaggtt taggtttttg gcagaaaata agttatattt cttcaattac ttattggtat
    18241 gcaagtataa aacgctttgt atatataatg gcacctattt tattttctgt gtttaatgtg
    18301 attgtagtaa aatgtacgtt acttcaagta ttggtatttt ggttgccaat gtatatttta
    18361 agtagtttat ctcttaaaat attttctcaa aatataagaa atactagatg gaccaatata
    18421 tatgaaacta taatgtttca gtctttaatg ccagcagtaa tacttgaaac gtttgcaata
    18481 tctaaaaata aattttcagt aacaaataag agtaaattag aagaaaatag aatgtataag
    18541 tttttacagg gaatacctta ttttatttac atggtcttat ctatcatagg aattttaaaa
    18601 atgtttgtag caatatttaa aatgagttca atgacatatt cagttgtatt attttggttg
    18661 attggaaacc tttttaacct tgttatggct acattattta tatcagggag acagcagtta
    18721 agaaagtctg agcgttatat tgctgaaatt gattttaagt taaagcaaaa ttcttatgtt
    18781 ctttcttcaa aaacaattga tatatcagaa aatgggtttg catttttgct agaaaatcca
    18841 gaatatattt ccccagaaga agagtttgag gtagagttta gagaaaaatc tggaaatgaa
    18901 atgtatatag ctaatatgaa agcaaagatt gttaatgttg tggaagtaaa ttcaaagtgg
    18961 aaatatgcag cttatattac acatattgag gatagtgaaa tagataattg gatgtgtatt
    19021 gttcacgata gaatacctac tttaccaatg acaatatcta atcaactagg tttctttgat
    19081 gatttgcaaa ttaatgtaaa aaaacgtatt gaaaaaacta gaacattatc acgaagaagt
    19141 ccaagaatca atatgaattt tcagatggat ataaaaaata caggtaaatt aagaatagtg
    19201 aattttaatt atcaatatgt attattaaat tttgagaata aaagtattta tcctaaagaa
    19261 atagcattgg aaattaatga aaatattgtg ttagaatgtg atttgtgtga gggtaaaatt
    19321 gatgaaagag gtatcttata taaagtaaat aacattgaca gcataatgca aaatttcttt
    19381 ttaagagatg aaatgatgga ttggatatta caaaataaga caattcttgt ttcaaagcct
    19441 agtgaaaaga aggaaaaaag tatagatgag tttgaaccca tggaatatat ttaggagaga
    19501 tgaaatgaag actttaaaag tgtttttttg ttctacaaaa tatcttattt tggtgtttat
    19561 tttttttata aatactactt ttataagttc agcaacaagt gataaaattt tagatttatc
    19621 ctttaaaaag atagagactg acttaagttc taagattaca tatgaggata caggtgtaag
    19681 aatagaaacg gatagttcta aaagcgataa ggaacgttat ttatatattt accaaaatat
    19741 aaaagaaaat tggagcatgt ataataattt ttatattgaa attcaaaata aaaataaaag
    19801 ttctcagaga ataaatttaa gcatacaaag taaaaatatg cttgaattta gattaaaaga
    19861 aggtagtgaa gtatttttag aaggtaaaaa tataatatat tcagataaaa taaaagaagg
    19921 atgcatagaa gtaccaggtg aatttgaagg caaaatttat gtgaacttta atagccttat
    19981 aaatgaggaa agtaatgttg tgctggactc aaatatgctt tcaaacattg ttagttgggg
    20041 aattacgttt attccatctg atgaagaaca taatattgtt attataaaaa agatatcttt
    20101 attgtctgaa gagaaattaa gatttttaaa taatattaaa ataattggcg atgaagaagt
    20161 acaaatacca gtattgggtc aaagtataag tcagtatgaa gtattaggtc ttaaaagtga
    20221 ttctaaaatt aagtactctc ttatgggaaa acaagataat gtgagcatat ctcaaaaagg
    20281 taaattaaca ttaagtaata aagccaaacc tggacaaatt attttacaag ttaatgttga
    20341 tgataagttt aaaattggaa agaaaataac attaactgag tcttggagtg ttaataaaaa
    20401 agataaggat ggtgttccat atacattagt atatcctgag caatcaccaa cagtacaaga
    20461 tatgaaaaaa ataaacttta tgaataacat cataacgttt gttcgaattt tatttgtatc
    20521 tctagtaata atatgctttg gaatatattt atattggaaa aaatgttcaa aaactaagta
    20581 aagagaggag gttagattgt gttattttca agtttaattt ttttatttta ttttttacct
    20641 ataactttgg ttttatatta tttatttagg tttaaccgaa cgatacagaa catgatattg
    20701 cttgctgtta gtttattttt ttatgcttgg ggagagccaa aatttgttgt tatcatgata
    20761 gtttccataa taatgaacta tatatttggt ttactggtag atagatatag ggaaagtaaa
    20821 ataaaagtaa aagttttttt agttttgatg tgtgcataca atataggtgt tttatttata
    20881 tttaaatatc tagcttttgc gctaagaaat attagtgaac ttattagtac tgaattgact
    20941 atacctagta ttgttttacc cataggaatt tcatttttta catttcaggg aatgtcttat
    21001 gttatagatg tatatcgtag acatggagaa gtccaaaaga acccttttta tgtgggattg
    21061 tatatagcat ttttcccaca attaatagca ggtcctatag ttagatatga gagtgtggca
    21121 gaacaaattc tcaatagaaa agaaacttgg aataaatttt ctattggtac ttgtaggttt
    21181 ataacaggtc taggtaaaaa agtattaatt tcaaataaca tggctatagt agcagactat
    21241 atttacacta tgaatagtca aggagaaata gctgcatcac ttgcatggct tggttcaata
    21301 gcctatactc tgcaaatatt ctttgatttt tctgcttatt cagatatggc tataggctta
    21361 ggtcttatgt ttggttttaa atttgaagag aactttaatt atccatatat atcaaaatca
    21421 attagtgagt tttggagaag atggcacatt tctttgggaa tgtggtttaa gagctatata
    21481 tactttccgc ttggaggttc tagagtagca aataaagata ttatgattag aaatatgttt
    21541 atagtgtggt tattcactgg aatatggcat ggagcagaat ggacatttgt aatttggggg
    21601 attttaaact ttgttttcct aattatagag cgatttataa tgtttgaaaa gattgaaaat
    21661 cacaatttta taaaacatat ttatgcttta ttagtagtta attttggatg ggtattgttt
    21721 aggtcaccta atttaaaaga agcatataat tattttaaag ctatgtttgg aggcaatgga
    21781 gctatatgga gtgattatac atatatgttt ttaaaagaat actgggtttt ctttatattt
    21841 gcatttattt ttagtgtacc tattgcaaag aaaatcaata agtttgttgt tgaacaagct
    21901 aaatatagta tgatatttaa cgtattttat ccaatctcag ctattttatt gttttttatt
    21961 tctgtgacat atttagtaac tggaagctat aatccattta tttattttaa tttttagttg
    22021 ggaggaaaag tatgaataga ttttttaaag taagattaat aactgcaatt atgttcttaa
    22081 ttattttatt cgctttctct atctttaatg tttgttattc tggaaaagat acgatacagg
    22141 aaataaaaaa ttcaatagct gaaaaaagta gtttaaaaga tacaatatca tctattgata
    22201 atacagtgaa tgacaaagta atatacaaaa atacgttcat tgaaacttat ggctatttac
    22261 aaaaattaat gggtaaaaat gagttttcaa aatttagtgt tgtaaaagat acttctggaa
    22321 agatgcatta tacatatttt gctaaagctc caaatccagt tggtgtgata gcagataggg
    22381 taagaaaatt ttctgatgaa atggaaaagc aaaacactaa attggtttac ctaatgactc
    22441 cagataagta tataaaagga gtgacaaaat ttccaaaagg aataccttat tcttatagta
    22501 atgagacagc agataatttt ttaaatcaac taaaaaagta taatgtggac tatattgatt
    22561 ttagagaaaa tattttaaag agtggtattc caaaagatga tttatttttt aaaacagacc
    22621 atcactggaa agtagaaact tctttttggg catttggaga attagttgag caacttaata
    22681 aaaaatacaa tatgaattta gatgaaaatc attattatag agataaggac aattataatt
    22741 caattgtata tcctaagtca tttcttggtt ctatgggaag aaagactggt atcttatatg
    22801 gtggtataga tgattttaca ttaatttatc ctaaatttaa gactaattat acttattata
    22861 cagattctaa atctcaaaaa tttgaattaa aaggaaggtt tgaagagagt ttaatattat
    22921 catatccttt taatgcagac cttgatttaa tgaatggtca atcagataag tattttacat
    22981 atttattggg gaacagacca ttagttaaaa taaaaaatat agaaaatcca gatggattaa
    23041 aagtgctgtt tgttaaagat tctctaattg taccaacagc agcatttttt tcctctgttt
    23101 gttcatctat agatatgata gaccctagat attatgatgg agatatacta gaatatgcaa
    23161 aatcccatga ctatgacttt gtttttatat ctgtatatcc ccaaaatctt acaaaagaat
    23221 tttttccgtt ttgtgagtaa atttctaaag aaattttctt ataaaataaa aaataaatgg
    23281 ctaactggtt aaaagttagc catttatttt aagtgttact ctaagaaata ttttttttac
    23341 taaatacttc tttatctatt tttccaagtg attttcctac agtaattgat gataccatag
    23401 caccattaac atttatcatg gtacgtccca tatctagtat agggtctatt gcaagtatac
    23461 caccaactaa tggaaaataa gaaccaagac caactccaga gataacaact gaaacggcca
    23521 ttgtagcagt tcctggaagc ccagctattc caaacgaact aattgttata acaattaaca
    23581 acataacata aaagctcata tccatgttta ctccagccat attggcaagt gttacagcca
    23641 ttaaagcagg atatatacct gcacaaccat tcatacccat atttgcacct aaacttgctg
    23701 taaagcttga tacaccctca tcaactccgt gattttgtac tagactttct atcgttacag
    23761 gcaatgttcc taaactagaa cgtgaagtaa atgccagaag taaagcttca gttgaatttt
    23821 ttatataagt aatgggattt agaccattta aagttataaa aattaaatgt ataataaata
    23881 atattgcaac tgatatataa agagctaata taaactgtaa aacagatact attgaactaa
    23941 taccttgtga agttattgta tttgcaagca gagcaaccac agcataaggc ataaatttaa
    24001 taacagtcat agacacactt acaattattt tgtaagatgc ttctataaaa tctataaatg
    24061 gttttattgt ctcaaaatgt tttttactta atctctttat tgaggtagct ataaatgtag
    24121 caaatattat tgtagctaca atatttccat ttgccatagc cattacagga ttagatggta
    24181 gtaatcctct taaagtatcc acaagagagt ttatttcttt aagttcttga ttttcaacta
    24241 aagtaggcat attagctcct acacctaact tcattatatt tgcaacgata attccaacaa
    24301 ttgcagctat agcagtagta gcaagaagca tacctaaaga tttaaatgtc agagttccaa
    24361 gattgttatc tttcatattt ataattactt ttataatgga taagaaaact aaaggtacta
    24421 ctaacatttt taataagtcc ataaatccag aaccaaatag gctataccac ttacttactt
    24481 cttgtagcca agtgacttga gttgtatcat taggaaaacc agcaaaagct tgtattatga
    24541 ttcccaatat gagtcctagt actgtagcat atattgttct caatgaaaat tttatttttt
    24601 tcttttcaaa ttgtctaaca ataaagaacg tacctagaag taatattatg aaaaaaattg
    24661 ttttaaaatc tgtaatcata agaaattgtg ataaaaatga actttccaat atttttctcc
    24721 ttttatttaa aaataaattt ttctaagttt aaatgatatc aatatattaa aaaaatgtca
    24781 atattatatc acaatttact taaatagagt aaaacaagtt gattttgaaa aatgtgatta
    24841 agtacagaaa aatagggtat aataaatgtt taaagaattt gaattgtggt aagaaaataa
    24901 aaaatagctt ttataagatt tgatttcaaa ttaaaatatg ttaaataaat aactaaaaat
    24961 tattataaaa aatgttttca tagaatatat atcaaatgaa aagcttaaaa taagtgaaga
    25021 aattaactag acatcaaagg agagataaaa tgagagtaat aataattggt ggtgttgcag
    25081 ctggtatgag tgctgcagca aaattaaaaa gaataaaacc tgagtatgaa gttgttgttt
    25141 atgaaaaaac tgaaatagta tcttttggtg cttgtggatt accatacttt gttggtggat
    25201 tttttaatga tgctgatgaa ttattagcaa gaacaccaga gaaacttaga gaagctggaa
    25261 ttgatttaaa tatatttaga gaagtcgttg aggttgatag tgaatctaag aaaataaaag
    25321 taaaaaacat aaaaacagat gaaatatatg aagattacta tgataaattg atgatagcaa
    25381 ctggagcaag aagtattatg cctcctataa aaaacataaa attgaagaat gtttctacat
    25441 taaaaagttt atatgatgga gaatatctaa aaaaactttt aagtaatgaa gataataaaa
    25501 gagtgacaat aataggagct ggttttatag ggttagaagc agttgaggca tgtaagaaat
    25561 taggtaaaga tgtagatgta attcagttag aagatagaat attgccacaa gtatttgata
    25621 aggaaatgac ggatgtatta gaagaagaaa taaatagaca taatgttaat ttgcacttag
    25681 atgaaatggt tgtagaactt tgtggagaag ataaagtaga aaaagttata acaaacaaag
    25741 gggaaataga tacagatgta gtaataattg ctactggcgt gagacctaat accgagtttt
    25801 tgtcaaatac taatataaaa atgctaaaaa atggagctat agtagttgat gagtatggaa
    25861 gaacttctgt agaagatata tactctgctg gagattgtgc aactataaag aatatagtaa
    25921 gtaatgaaaa tgtatatgtt cctttagcta ctggagctaa taaattgggt aggatagtag
    25981 gagaaaacct agcaggaaga gaagtatctt atcaaggttc actatcttca agctgtataa
    26041 aaattatgga tatggaggca gcatcaactg gaattacaga aaagcaagct aaagatttag
    26101 gcataaatgt caaatctaaa tttatttctg attataatca aactcattat tatccaggaa
    26161 gaaataaaat atatgtaaag cttatttatg atgcagatac aaaagttatt ttaggcggac
    26221 aagtggctgg gtttaaggat gctgtccaaa gagcaaatgt attggcagct gctatctttg
    26281 gaaaaatgac cacaaatcaa cttggaatgt tagatttatg ttatgctcca ccatttgcaa
    26341 gaacatggga tgttttaaat gtagctggaa atgtatctaa ataatattta gattatagct
    26401 aatatagttg acaagcaatt aagggaatga atttacctaa actattttta tcatggtaaa
    26461 attcattcct aaatttttat tccaaatttt tagaaataag gattccatta aattattcat
    26521 caaaatttaa acgcaagctt aatctatctt ctccaagaat agtattttca aaaacattag
    26581 tagcattttc taaatacata ctaggtatgt ctaaagaagg actaaaatga gtcagtaaaa
    26641 gttgtttgac atttccaagt tttgcaagat tagcagcttc tctaaaagtc atatgtttgt
    26701 ttctaacagc ttttgatata tctaaatcat caccatacat ggcttcacat ataaataaat
    26761 cactatcttt tatgaaatct ggtattgatt ctataggtct tgtatcggtt gtaagactta
    26821 tcttaactcc ctttctcaat tctcctaaga ccatatctgg agtgtattgt tttgaatcta
    26881 aaactatatt ttgaccttct tggagtttgt tccataatat tttaggaact tcgtttttag
    26941 ttgccttatc tatatcaaat ttaggatttc ttctaaaatt aaaactgtat cctaaacact
    27001 ctatagagtg ttctaaactg attgttgaaa tttctaaatt tcttaaaact ttattatcta
    27061 aagaaaaatt gccttgaggg ttttctataa tttttaaagt gtatggtaca tattctacta
    27121 aatttctcat agaacgtata caatcgacta ttccaactgg accaactata gttaaatcac
    27181 ttgtacgacc actattccca attgttgata aaagtcctaa aagtccaaat atatggtcac
    27241 catgtaaatg ggttatacaa atcaagtcta tatctttaaa gccacatttt tttagcttca
    27301 tagatacctg agtaccttca ccacagtcaa ttagtatttt tctaccttta tagtttataa
    27361 atacagagga tagaaatctg tttggcatag gtacatttcc accacatcca agtaaagtta
    27421 aatctatcat tttttcccct ttctaaaatt aacacaaatc tgttgtttgt ttttattatg
    27481 atatcctatt ataattattt taacattaaa tataacaata aatgtgtatt tgagtcaaga
    27541 aatattgtca aataaagttt ttatatagca atgtataaaa atttggaaaa cgaaaagtga
    27601 aaattatatt taggagtatt taaaggagga aaatttaatg aaagtattaa agaaaattac
    27661 tattttgtca ttaattacat taaccttagt tggttgtagt aacggagaag ataaaaaagt
    27721 ggttgaaggt tctaagaaaa ttcaaaattc taaaaaagat gaacagccta taatagtaca
    27781 taaagatgca aatatattga aagataggta tgagccacca aaaggattta aaagagttga
    27841 tgtagaaaaa ggtagttttg aatcatttct taggaatact aagctaaaaa attatggaga
    27901 gaaagtaaag tattatgatg gaagaactaa ggaatcaaaa ggtatttatg atagtgtatt
    27961 tgatgttgat attggcaata gagatttaca tcaatgtgca gatgcaatta tgcttatgag
    28021 agcagaatac ttgtatcaga ataaactata tgatgaaata agttttgatt ttgttgatgg
    28081 ttttaaggcg aagtattcaa aatgggcaca aggatataga atttcagtaa aagatagtgg
    28141 ttcaagctgg tataaagcaa ctgaatattc aacatcttat gagagtttta gaaagtttat
    28201 ggatatagtg tttgcatatt caggaacgtt atctcttgaa aaagaattgg agcctgtaaa
    28261 agttgaggat atgaaaatag gagatgtgtt tatagttggt ggaagtccag gacatgcaag
    28321 tataattatg gatatggcag aaaatgaaaa gactggtgaa aaggtattta tgatagccca
    28381 aagctatatg cctgcacaac aaacgcaact tcttattaat cgtaaaaatc caaatttgag
    28441 tccttggtat agtttagatt ttgaaggtga tttgacaata cctcaatgga cttttaaaag
    28501 agaccagttg aaaagatttt aaaataataa ttttatataa taaaagaaaa atatagatgt
    28561 gaaaaaatgt aattagatta aaactaattg gatatggaca aaattaaatt gatagataaa
    28621 tattaaaaat tacactaatt aatcttacta tagtttaata tttatctaaa aagttttatg
    28681 taaaatataa attttatatt aaagctaaaa ctaatttgag gaggctaatt tatattgaag
    28741 attagaaggt atttaatttt ggtaattata attctaatta caacaagtca gagttttatt
    28801 tcttatgggc tagagcgatt tgaagaaata accatttttc acactaatga tatacatgga
    28861 agatatgctg aaggtgatga ccatatacaa ataggaaatc tagccacatt gaaaaaagaa
    28921 actccaaact caattttagt agatgcagga gattgtttgc atggacttcc aatagtaaat
    28981 atggatagag ggaaaagtgc aattgaatta attaaatgtg ctggatatga ctatattact
    29041 cctggaaatc atgactttaa ttatggaaaa gatagactgt ttgagttatc taaaagtgct
    29101 aatttaggag agcatacttt aaaattttta tcatcaaata tttttgaaaa tggaaaaaaa
    29161 gtttttgata gtaatgatat aaaagaaata gatggaataa aaataggatt ttttggactc
    29221 tcaacccagg aaacaaagag caaaactgga tttaaaaatg tggaaggatt agagtttaaa
    29281 aatcctatag aaagtgcaac agaacaagta aatgaattaa aaggaaaagg tgccaatgta
    29341 atagttgcaa tatgtcatat tggtacaaat agtttaagta agccaaatag tatagatata
    29401 gcaaatgagg ttcaaggaat tgatttgatt attgatggac acagccacac taagtttgaa
    29461 aatggtaaac aagtgggtga tacattgatt gtaagtgcag gacaatattt agaaaatatt
    29521 ggcgagataa aactaactct ggatactaat gatgtagaaa atataaaaat agaaaataaa
    29581 tcagttagat taattggaaa agaagaagca ttaaagtata agcttgactc cgctgtaagt
    29641 aaaaaggttg atgaaataaa agaacaacaa gaaaaaatat tagataaagt tataggggta
    29701 actacaaata ctctagatgg ttcatatgaa aatgtaagga caaaagaaac taatttgggt
    29761 aatttaataa gtgacatttt actagataaa actaaggctg atatttcttt atttaatgga
    29821 gggaatattc gagatacgat agaaaaaggc aatataacaa gaagagatat tgtagatgta
    29881 tttcctttta gcaatactat agttaccaaa gaacttacag gagcacagat taaagatgta
    29941 ttggagcatg gagtaaaact ttatccagaa aaaagttcag cctttttgca agtgggagga
    30001 atttcatatt actttaatcc taaacaaaaa gaaggcgaaa aaataacaaa tattcaaaaa
    30061 gaaggaaagc cattagattt aaataaaaag tatgtagttg caacgaatga ttatatagca
    30121 tcaggtggag atgaatttcc ttgtttttca aaagagccag tactaaagga atttggaaat
    30181 ttagagagtg cagtcattga atatatagag tacaaaaaag aaatatcaaa aaatgtagat
    30241 ggaagaattg gtattaaatc taaagatgag ttaataagca agaatcatga gagtgatttg
    30301 atattaagtg aaaatgaaaa aaatatcaat gataatgaag agaatctatc taataatata
    30361 aaaagctctg atagtgataa aaataaaagc ttagataaaa caagtgctag agaaagttct
    30421 aatctaggaa tatctaaaag agaagctaaa gaaaaatctc ctaaaacagg agatttaggt
    30481 ttttcaaata gtataattat atttatagtt tcaagtacat tgatatgttt attgaatttt
    30541 aatcaaaaag agttaaagga taaaaagtca aaataaaaat taatgagcaa aaactagtag
    30601 atagtaattt aattttagat aatagtaaac agcaaaggaa attagttata taaatactaa
    30661 tttcctttgt tttatctttt aacatgatta cttttaaata aaattgtatt ttatgaaaaa
    30721 taattacact aaatggcata gtattatggg gatttttagt ttttattcaa gtaaatgaaa
    30781 tcatattaac aacttctgat atatttacaa attgataaaa aaatgatttt attagtatga
    30841 tataatttag ctagtatata tgatgatgga ggatataggg atgaataata tattactttt
    30901 ggaagatgac aaatctttaa atagaggaat tagttttaaa ttaaaaaaag aaggatacaa
    30961 tgtttttagt gcatttggta tagaagaagc aaaaaatatt ttcgcaaaag aagagatatg
    31021 cttaattatt tccgatatag gtctacctga tggtagtggt tttgactttt gtgaggagat
    31081 tagaaagaaa agtgatgttt acataataat gttgacagct cttgatgaag aggtggatat
    31141 tgtcacagga tatgatttag gtgcagatga ttatatcaca aagcctttta gcttgatggt
    31201 attaatatca aaagtgaatg cgcttatgaa aagagtaaat acagtaaaaa attatactct
    31261 attagtttgt gatgatttat tcttttatta tatagaaaat aaattaatta taagaacaga
    31321 tgataaggaa gaagagataa ttttaagtaa gacagagaca aaactattaa aatatttgat
    31381 ggaaaattct atgcagacat tgactaaaga gcaactgtta gaatctttat gggatagtag
    31441 tggtaatttt gtagatgata atacgattgc agtaaatata agaagactta gacaaaaggt
    31501 tgaaaaaaat ccatctgaac ctaaatatat aaaaacagta agaggggttg gatatatgtg
    31561 gggagaaagg agtataaaaa aatgctaaaa aatatgaacc tcaaagatgg tttaagtgtg
    31621 aaagatgatt atataatgag aagagttatt gtatattctg ttttatctgt actaatagta
    31681 acccttgtat ttatactttt tgaatatagc agattacata aattgtatga acatcaaata
    31741 aaaaatactc aaaatattgc tggaatattg attgaaaaat atcctaatga tgaaatagat
    31801 atagtaaatt ctatttataa ttctgattat agtaaggtgt ctactggaga aaaggcattt
    31861 aagaaatttg gctatggatt ggaaaataaa atgtcaaatg ataaaaattt tagcttatac
    31921 ttaaaatctt tttttaagga atcatttttt atttttttaa tgatgtttat agtagttata
    31981 ggtataatat attattttat aagatacata aataaaagat tgagtaaaat ttattttata
    32041 gtagataaga tgtcacaaga aaactacttg aaggacacta ctgagtcaga agaaactggt
    32101 tatctagtag aatataatga atactttaaa gaaggcatat ttagtaagat aaataactgt
    32161 ctatatgagc ttaatagaag tttaaagata aagtttatta aattagaaaa agaaaaagaa
    32221 agtgtaaaat cattagtaac agatatttcc catcaattaa aaactccttt agcatcctta
    32281 aaattataca atacattact tatagaagaa gaattagaag aagatgaagc caacgagttc
    32341 ttattaacca ataaggcttc tataaataaa ttagaaaatt taataaattc tttagtgaac
    32401 atatcaaggt tagaaattag catgataaac ataaaaaaag aagataatga tataaaaaat
    32461 actattctaa atgcagtaaa aagtgttacc cctaaggcta agagcaaaag tataacatta
    32521 aatttaaatg aatttaacag tagaattata ccacatgata aaaagtggac tgaggagagt
    32581 atttttaatg tattagacaa tgctgtaaag tatacacagg taaatggaga aataaatata
    32641 tctgtagaag aaactgctaa tttctttaaa attataatag aggataatgg catagggatt
    32701 gaaaaaaatg agtttaataa tatatttaaa cgtttttata gaggttctgc tgatgaaatt
    32761 gaatctgtag aaggtagtgg ggtaggattg tatttaagta gaaagatttt agaagaacaa
    32821 ggtggaaata ttatagtttc ttcaaaagta ggaataggga gcaagttttc tttgtttctt
    32881 acaactatgt aatattaaat gaaaggaaaa tgtaagcctc tacatttatt atataagtat
    32941 aaacagctcc agaaataaaa aattaataaa atatggggta atataactta gaatgagagg
    33001 ataattttat gaaaatacta tatactgaaa atttatcaaa gcattatgga aaaggagaaa
    33061 gcttagtaag agctttggac aatgttgact tagaaataaa tgaaggtgaa tttgttgcaa
    33121 taataggaaa atcaggttct ggaaaaagta cacttttgca tatgattgga ggtcttgata
    33181 tacctacatc tgggaaggtg tatatagata ataaaaatat atttacatta aaagaagaag
    33241 aacttgctgt atttagaaga agaaaaatag ggtttatatt tcaaagctat aatttaattc
    33301 catctcttaa tgtatgggaa aatgtagtgt tgccaatcgg tttagatggt agagaggtag
    33361 atgaaccttt tataaaagag cttttaaaat cattaggact tgaaaataaa cacgacgttt
    33421 taccaaatac attatcaggt ggtcaacaac aaagggtggc aatagcaaga gcattagcta
    33481 caagaccagc tataatactt gctgatgaac caactggaaa cctagattct aaaacatctg
    33541 atgaggttat gagtatatta aaatctatgt ctaagaagta cagtcaaact ctagttatga
    33601 taactcatga tgacagtata gcccaaatgg ctgatagagt tatattcata gaagatggca
    33661 gggtgtcaaa ggttggtgat aaaaatgatt aatactacta aaatagcaaa aagtaattta
    33721 aagcaaaata aatcaaaaag tattctaata ataattacta taatactttc aaccttaatg
    33781 ctttcttcta ttggaattta tattgttgat gcaggagcat atcaaaaaga aaatactata
    33841 aagtattcag gtaattatca aggtatatta gcaaatgtag acgaaaaaca agctgatata
    33901 ttgagtaatc atgcagatgt tgaattaact ggtaaaatga atggagtagg tgttgaaaaa
    33961 ctggaagatg attcaaatat ttctctagca tacatgaatg aagatgcttt gaaattaaat
    34021 agttttgaat ttatcaaagg aaaattgcca tcaaaagaga atgaaattgt gttagatagt
    34081 ggagctttaa aggcacttgg ttataaaaat aaagatttag gtgaaaagat aaaaatatct
    34141 tataatgact ataaaagtga taaaaaaatt gaaaaagagt tcattataag tggaatctta
    34201 aaaactagcg aaatatctga agcaggtaaa tattattatg ctattatatc agaatcatat
    34261 atgagaaata ctagagatat gtcacaagaa gattttaata tatatgtgaa attaaatgat
    34321 aaaagtaatt tatcgataga acaagcaaaa tcaaaattaa ataagattgc taatgatgta
    34381 ggattggaca ctattaatgt agctgtaaat gaaaactata taaatgcttt aaaaccagat
    34441 atggaaacaa ttatgggtgg agtatttgta gggttagtaa ttgtattgtc tagtatttta
    34501 gtaatataca atatatttta tatttcaata gttacaaagg ttcaagaatt tggaaagctt
    34561 agagcaattg gtgcaactaa aaaacaagta aaaaatatag tatttaagga aggcttcatc
    34621 ttagcaggaa tagcaatacc aataggcata attttaggat atgcactttc aaatattata
    34681 ataaaatcag ttatggatat agatgtaaaa tcatcacaat taccagttat attattggtt
    34741 ataataataa gctttatgtc tgttgtgtta tcacttttaa agccaatgaa agtagcatca
    34801 aaagtatcta tagttgatgc agtgagatat acaggaaata aaataagtaa taaaaataag
    34861 agaaaaggtt ataaaaatat aaatttaaag agattatcac aagctaattt agaaagaaat
    34921 aaaaaacgta cttatatgac attggtatct cttatattga gtggaactat atttataaca
    34981 gtatcaactg ccttagaaag ttttgatgct gagaaaatgg caagagaaca ctttccttat
    35041 gatatagaag ttagactatc tggttatgaa atgaacagtg ataaaaatcc taaagataat
    35101 ttgaatattt tacaaatgga taatcctcta agtaaagatt tttttaatca aataaagaac
    35161 atggaaggaa ttaaaagaat agaatctgca agaagtgtaa aaattggtat ggaggactat
    35221 gatgttgaat ttaagtatga ccttttacaa agtataaatg aaaatgatgt aaaatcatta
    35281 aacaagaata taatagatgg aaaaataaat cttgaaagac ttcaaactgg agatgaaata
    35341 gtaattactc atgttgatac agcaaaagaa atgggagtaa aagcaggaga taaaataaga
    35401 ttaactttat atgatggaga taaaaagata aaaaaagaat ttaaagttca agcaatagct
    35461 atgggggttc caagttttgg tatagggaag gattttattg atagaacttt gaaatacgat
    35521 tcaactagta gcttgggaat atatacagaa gaaggaaagt atcaagaaat caaagatagt
    35581 atcaagaaga ttgctaaaaa taatggattt ttagagactg attttataga tagtagaatt
    35641 gagtcaaata aggctactat ttcatttata aagataatgg gatacacgct aacaggcata
    35701 ataggtgtga ttggatttat gaatcttgtc aacacaatga taactagcat agttactaga
    35761 aaaaaagaac ttggtatgct tcaagctatt ggtcttacta ataaacaatt agtgaaaatg
    35821 cttaattcag aggcaatgta ttacacttct ggaatgatga taggaagtat tctatttgga
    35881 ggtatattag ggtatattgc tgttatattt ctaaaaaaga ctggattatc ttatgcaaca
    35941 tattctttgc caatagtacc aatcttgttg atgattgtgt gtattcttat agctcaattt
    36001 ataataactt atttgatagg acgaagtttt aataaagaat ctcttattga tagagtaaga
    36061 tacagtgaat aaaatacaaa tatttatggt tagtttaact agcaaaaaac atataaaaaa
    36121 atatagttaa agagattcat aattaaatag gttaaagcat agtacagagc aaacaattta
    36181 agcagatgtt tgctctgtat tttattatgt aatcaataat atatctaata tactgatatt
    36241 attcatgtta gaaaatgtac cggtacaatt ctaaaaaagt ataaaattgt accatataaa
    36301 atgaaaattt tgtataataa tcgtatagaa aataaatagg agctgatata tatgaataag
    36361 aagattaatt atgcaccagg accaacagaa actagggaaa atgtaagatt agcaagagca
    36421 gaaaaaacaa caaacccaga tatagatgtt gattttgttg agttttataa aaagacatgc
    36481 gagaagtttg gtaatatagt tggtacaaaa aacgatgttt acatattgag tggtgaagga
    36541 atactaggac ttgaagcagc ctgtgcatca cttacagaaa aaggagatag agtacttgta
    36601 attgacaatg gtatatatgg agaaggtttt aaagattttg taacaatgta tggtggggag
    36661 tatgtgcttt tttcaagtga atatactaaa agtatagata ttgatgaatt gaaagcattt
    36721 ttagatgagg atagtaattt taaatatgca acagtagtgc attgtgatac tccaactggc
    36781 gtattaaatg atgtaagcaa aatatgtcca ttacttaaag aatatggaat attaactgta
    36841 gttgattctg tagcaggtat ggtaggagaa agattaagtg tagatgaatc taaaatagat
    36901 ataatattag gtggttcaca aaaagctatt tctgcaccag caggacttac tatagtcgga
    36961 ataagtcaag atgcaaaaaa ttgtataaaa aatagaaaaa ctgatgtaat aggtttttat
    37021 tgtaatttaa atatatggga aggctattat gaaaaaaaat acttccctta tacaatgcca
    37081 ataagtgaca taatggggct tgataaagca cttgataata tattagaaga aggaatagaa
    37141 aatgtattaa atagacatga aaagatagct tatagtgtta gaaaatctat tgaggaatat
    37201 ggtctagaac tatttttaga agaaggatat tctaatacag tcacagctat aaaaattcct
    37261 gaaagtatag gagctttaga acttactgat tatatgctta aaaattataa tacacttgtt
    37321 gcaacatcac taaatcaata tatggatact attttaagaa taggtcatat gggagagaat
    37381 gcaaatctaa ataaaataga acatgtacta aatgttttgg ataaaagttt aagtgcatta
    37441 ggattcaaag gaaatggaaa tttgttaaat ttatttaata agtattattt ttaatagata
    37501 gggggatata gtatggaagt tacagctaag caaaaactta caacgaaaga catggtggaa
    37561 acctcactat taatagcatt agtatttatt gcaactaaat ttataaacat aagattacca
    37621 atatctataa atggtgggct tgtgcattta ggaactgcaa tgttgttcat atcagcaata
    37681 gtatttggag gtaaaaaagg tgctttatca ggggctattg gtatgtcact ttttgatttg
    37741 atatctgggt ggactttatg ggcaccgttt acctttatag ttagaggagt tatgggttat
    37801 ttacttggaa agattgcatg ggcaaatggt aataatggga ataatttttt aatcaatgta
    37861 ataggaatat gtgtctcatc aatttggatg ctaattggat attatgtaac agaagttata
    37921 ctttatggta attttatagt acctcttact tcaataccag gtaatttaat gcaagtattg
    37981 ataggactta taatagcgct tcctatttct aaggtattga aaaaatgtat aaaataaata
    38041 taaagtgaac taaaatttat gatagcataa attatactgt ataatattag gatggagtac
    38101 ataattaaaa aattttagta tagaggttag ttcacaatgt tatcagttac aagatattca
    38161 atcaaaaatg tccgtttaaa gccgtgggta aagtttattt ggtattttga gacaaaatca
    38221 aatatactgt taaataataa gttacttccg acagacagta tagatattat tctcaatctt
    38281 tctgatgtta tggaatataa aattgaaaat caagactata ctgctagtaa tatgcatttt
    38341 aatggaataa gggataaaca tggatttatt attcaacatg gaaatatacg tgttataggg
    38401 atttcttttt atccatttgg tctctatcca tttttaaaga taccaatttc agagtttaac
    38461 agacaaattg ttgacctaga agcagtatca cagctatttg caaaaaaatt ggaagagtca
    38521 ttaaatccaa tacaatcagt agaaaaaatt gttttatatt tagaagaagt attattatcc
    38581 atattagaag aagatttaat ttcaaataaa tatgtaaagc ttctaaattc atttatatat
    38641 gttaatagat atagcaatat aaaagcattt tgtgatgata caaatataaa tattaaaaca
    38701 ttggaaagaa tctgtttaaa atatacagga tatacaccta agattttaaa gagaatttac
    38761 agatttaaaa tggcaagcaa tcaacttatt tataattata aagatgatga actttttgat
    38821 tttatttatg aaaatgagta ctatgaccaa gcacacttta taaaagaatt taaaaaattt
    38881 tcagggacgt caccaattaa atttagtggt gagaataaga cgattaaaga aaatataaag
    38941 tacagctatt tataattata ctgtcgattt tttacaatac ctccaattta ttttttgata
    39001 tgatatagga aaataaattg gaggttttac atatgaaaat tattacagaa atagttgagt
    39061 ttaatgtgga agaaaattta tcaaaagaag agtttataga aattgtaaat gacttagaat
    39121 taaaatttca ttctattcaa tctggtttta ttgatactga gcttttatat gatgaaaagt
    39181 ctcaaaagtg gattatgata caacattggg aatctataga agacctcaag agtgcttcta
    39241 gtaaaatgtt taaagattct tcaactgaga aatttagaaa agctttaaag aatcaaactg
    39301 ttaagatgaa tataatacca caaattaaaa gttggaaatt atagaactga atgtaaaaga
    39361 tagttaaata gtatcaaact aaattagcca aataacattt tgtatttaaa ttgttatttg
    39421 gcttttagtg tttattttaa tatgagtgtt atatactaga aaaaccaaca aactgttaga
    39481 gtgtggatta tcataatgtc atattcggga gctaaaaggc ttaatactga tttgccaatt
    39541 ggtcaatcca tgcatgtcca tctggatatg ctgttattaa agcatttaca agccaatccc
    39601 tttgctcttg tgataatagt gggactgtta catcgaagtc tttttgataa ccaagcctat
    39661 ctatgtatat tgatttgcaa aacaaaacca cttcgggagc catatatggg atattatctg
    39721 atgttatcag tctggcctta tccatatctc ttataattct ttgattttgg gaaaaaagaa
    39781 attttccatc tttctgtgga ttatatatca attcaataaa atcgaactgt aactgttcgt
    39841 cacactgtat attcatttta tagttatcat cctcgacttt ttttaattca ataaatgagc
    39901 aatcaggctt aatcgcaaaa acacaaattt gctctatatg ttgcttgtta aggtcgataa
    39961 taggtcgtaa taaataatta cccaagggct catatatatc ccatccttga ttaagcatat
    40021 actgaatgct cttttcttta tctgtgtcag aaatccatat gtcaatatca ctgtgtgggc
    40081 gaatatgcat attcgcaaat aattctaggg catatccacc acatattgaa tatgaaaagc
    40141 cacaatcatt aaaaaagttt acacaatctt taattagttt gttcatagca acctcctttt
    40201 gcatgtactg aaataaatta aatttttaaa aataccaact aaatgtttgt tttgtgtaaa
    40261 tataaaactg ccgatattaa ggcagtttta tatttactat ttagaaaatt tattaacaaa
    40321 tttttgatat tcacacatta tcttatcatt cataaaagaa atacccataa aagcagcttg
    40381 ctcaccaaac aaagtaaaac gttctatcat ctgttgtgga ttacctggat aaactgttgg
    40441 aatacaccat gtgtttagaa gaaaaccaaa catttgagca gcaatctctg gatttttaac
    40501 ttccatagaa ttatcttgat ttccttcttc aatcaatgct tttatccaag gcacaatatc
    40561 atttataatc tcatcaaact gcgtagctaa atacttagga ttacgcaata gtgatgatga
    40621 cattaaaaaa atgtcttgtt tattgtgctg ttcataagca tgaagcaacc tatattgtaa
    40681 tttttctaat gcggtctttc cttcatcatt ttttacctca tacccataaa atccatcatt
    40741 atacaaccga cttgtgattg ccttaattat ttcgtctttt gatttaaagt gatgataaat
    40801 agcacctctt gaaaggccat ctaaattatc tacaatgtct tgaatcgttg tctgctcata
    40861 ccctttttct aagaataact tatgtgaaat atcaagtatt ttttgtatag tattatcagg
    40921 ttcttttttt cttcccaaaa agctcactcc tcgaaacaat catttggtat gtttatctta
    40981 gcaaatacat aatgatttgt caatgtgtct ttttagcatg ttgaacaggt atattgccat
    41041 gctttaatta ttttattttt ttgccttata ctggactata tttgcagttt cctaatatat
    41101 ttggctcata tttaaatacc attatctaaa agcactattg ctgtacgaga ggcaataggc
    41161 attttagaag aaggaggagt tgagttaaag tatacatcat aatctcttaa gttttctttt
    41221 tttgtgtttc ctccaaactc taccatatca gtatttaata tcgttttgta gtttgtattt
    41281 tgaaactttg aaatatcaac tgattgtatt agattagggt ggaaattaaa tataaataaa
    41341 tatttgccct tactgtacac caaaatcttt ttatcattat ccatatgtat aagttcatga
    41401 ctattttgga taaaaatatc actgtatttt gtaagttcga gcatagattt atcaaaattt
    41461 aataattgtt tatactttaa gttgttatct tctgaaaggc tccattgtct tctggcatac
    41521 ttatagctcc aattgttgcc ttctcgtgga aaatcaatcc actcaggatg tccaaattca
    41581 tttcccataa aatttagata tccttcacct gctagagaaa aagttattaa tttaattaat
    41641 ttaataagag atattgctct attgataaca tggttatttg agtttatttc catattccag
    41701 tacatttcct tgtcagctag ccaaaaaata attgttttat cgccaactaa tgcttggtca
    41761 tgggattcac aatatcctat atttttttct ccaggtcgtc tagtagtaag ttcataccac
    41821 atttttccca aatcccagtc ttcatcagat aagttagaga tagtttttat ccaaaaatca
    41881 ggtactccca ttgcgagtct atagtcaaat ccaattccgc cgtcttttat agggatacac
    41941 ataccaggca ttccactcat atcttctgca atacttatcg aattaggttt aatttctttt
    42001 attaattcat ttgcaaattg gagatatgtt atagcttcaa tatctgtatt catactaaaa
    42061 tatttttcat aactatcaaa tgaaactcca agaccatggt tatgatataa cattgaagtc
    42121 acaccatcaa acctaaagcc atcaaaatgg tactcattta accaaaattt tatatttgaa
    42181 agcaaaaaat gtattacttc aggtttacca tagttaaaaa gttttgtacc ccaagcagga
    42241 tgatttccct ttgaacctga gtggaaaaat tgatgttcac ttccatcaaa ctcatttatt
    42301 ccttctaaag tattctttac agcatgagaa tgaactaaat ctaataaaac agcaatcccc
    42361 attgaatgag cagtgtttat gaggtttttt aagtcttcag gagtaccaaa cctagatgag
    42421 attgcataaa agtttgaaac ttggtatcca aaagatgcat aatatggatg ttccataata
    42481 gccataagct gaatggtatt gtatccagct ttttttatct taggaagtat tttttcagta
    42541 aattcattat aagttccaat agattcgctt tcagtagaca tacctatatg acattcatag
    42601 attagtggag aagttatgtt ttttaagtca aaattattgt cagtccagat aaatggagtt
    42661 tttggttgcc atatttgtcc attaaatcca ccaaaatctt tttgaattac tctttttata
    42721 tataatggta tacggtcaaa tgtttttcca tttgcagtaa cttgtacctt tacctctgat
    42781 ttgtgaggta agctattttg accaggaata aatatttccc aattaccaga cccaattttt
    42841 cttagaggat gagattttct attccaatta ttaaagtcac caattagtga caaactatct
    42901 gcatttggag cccattccct atagaagaat ccatcatttg tctgatgaaa tccatagtaa
    42961 agatgagcat ttgcaaaaga actaaaatct ttatagtttt tcaatgtttt tgatttcata
    43021 tcctcataat gtttcattcg cttttttata tcagattcaa atggttgtag gtatacatca
    43081 atatctaaaa ttttaaatct tttttgactt ttcattgtat ttctcctcca taataaatat
    43141 tacatctaaa aaataatatt tatctcatac tatcttctct gtataattga tatatattat
    43201 ataatcctaa taaaaagctt aagccaccaa tgattaattt cataatttac ctccctagta
    43261 tttatactta tatatcgtat tcatcaaaat ttaaaagttt gtttaaaact cttttaataa
    43321 tatcataatc aaagccttta taggaaagat gttgagaaat tttttgatat attttctttt
    43381 tatcttcttt ctttactctt tcataccttt tttttgctag atacatagca ttttcaaatt
    43441 cagtgtcctt gtctaattca gatactgctt cattgattgt acttctattg atacctttat
    43501 tatataaatt ctgcttaatt ctgtttttgc cacatttatt taggttaaca tttgtgttaa
    43561 caattttttg agctaataca ctgtcattta cgagattata acttttaaga aaatctatga
    43621 ccctctcaat tatattttca tcaaattctg atgatagttt ttcttttaat tttttttcag
    43681 attggtcagc atgagataaa atatttaatg ctttattttt agcttttata tacatttcat
    43741 catctaaaat aggttttaat tcttcttcat ttatttcatt cccttttttt aaattaaatg
    43801 tataaacaag ttctttaaat atagcaataa aaaatttatc atctacataa atattaactc
    43861 tatcatcatt tcttttttgt tgttctattt ttgtaattat acccataagt cctccttttt
    43921 tatatgtaat tatattaaca aaagcaacta ataaaattta acaaaaaact tatatttgta
    43981 tgttatattt tatcaaaatt aagacaaaat agtaaaaagt tatttttatt aaa
//
LOCUS       DAELJO010000009        43580 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELJO010000009 DAELJO010000000
VERSION     DAELJO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.009"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..597)
                     /locus_tag="KPB88_000440"
     CDS             complement(<1..597)
                     /locus_tag="KPB88_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052750.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYHPYTGGAGGGLAGGTGYSANDRHR
                     PGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWF"
     gene            complement(614..1615)
                     /locus_tag="KPB88_000441"
     CDS             complement(614..1615)
                     /locus_tag="KPB88_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0052751.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSAYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREIGVFDSKDQLL
                     AVSSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVVLATLRDIQDLDAKI
                     DTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTITIDDWVEDA
                     ESGFKSTVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSETRSELNVYVIN
                     GNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNLEEKVNGGLSNIATSVNELITYC"
     gene            complement(1616..2230)
                     /locus_tag="KPB88_000442"
     CDS             complement(1616..2230)
                     /locus_tag="KPB88_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0052752.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPAHLEHKFEMILFNENGLYAGAM
                     SSTGETVTIYPYTPKNIECFGEIILASGNNKSAERVTLYPQEVI"
     gene            complement(2232..3284)
                     /locus_tag="KPB88_000443"
     CDS             complement(2232..3284)
                     /locus_tag="KPB88_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0052753.1"
                     /translation="MYSGQSFSTLNNRTLNNINLPLYKNQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTIV
                     SHSDLLFVVIKDVTIDEDSKLNVSLIQALEVGIKYNLSANTEFKLIEEINGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCIEHIEEEKPIGPTITIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKAPAVSSAIFDIEVS"
     gene            complement(3277..3711)
                     /locus_tag="KPB88_000444"
     CDS             complement(3277..3711)
                     /locus_tag="KPB88_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0052754.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFDKDEKVIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVYLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNVTNAEFKDDNLSISLNVKTIYGEEEITFV"
     gene            complement(3704..4036)
                     /locus_tag="KPB88_000445"
     CDS             complement(3704..4036)
                     /locus_tag="KPB88_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0052755.1"
                     /translation="MADPINEFIGIMRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILVDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            complement(4050..5567)
                     /locus_tag="KPB88_000446"
     CDS             complement(4050..5567)
                     /locus_tag="KPB88_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0052756.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGTYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNVTEKA
                     KTVISEARKHLGKPYKWGGNGASSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            complement(5807..6442)
                     /locus_tag="KPB88_000447"
     CDS             complement(5807..6442)
                     /locus_tag="KPB88_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0052757.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKDVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDKIKDPSVIKNGWILIIP"
     gene            complement(6500..6976)
                     /locus_tag="KPB88_000448"
     CDS             complement(6500..6976)
                     /locus_tag="KPB88_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0052758.1"
                     /translation="MNKKIPALAILIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTDLESDFLQNYILKTDDNTV
                     GNAINNQELNGELLFDTIHPGDTLKGEIAFELTKDTKPKTIEIAYENVGKYTVFDL"
     gene            complement(7023..10412)
                     /locus_tag="KPB88_000449"
     CDS             complement(7023..10412)
                     /locus_tag="KPB88_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0052759.1"
                     /translation="MSSNAKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGVFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALAVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFNLIIGIVTGDSKKMMNG
                     VKQIFDGGVKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWKDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTIANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSFFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            complement(10416..10565)
                     /locus_tag="KPB88_000450"
     CDS             complement(10416..10565)
                     /locus_tag="KPB88_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052760.1"
                     /translation="MYLLFKYKGIMPSVSYRILRGEKEIVSVFMDQELDERKEEIKSG
                     AGRLF"
     gene            complement(10592..11008)
                     /locus_tag="KPB88_000451"
     CDS             complement(10592..11008)
                     /locus_tag="KPB88_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0052761.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSIFELYCKKLDDILEIECNAIDPER
                     FDEIRMNSMDVDSGNLENINVYELKVNTILESCPMFRNMEVVENLGLATPKDLLKKLL
                     LAGEIDNLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            complement(11024..11464)
                     /locus_tag="KPB88_000452"
     CDS             complement(11024..11464)
                     /locus_tag="KPB88_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0052762.1"
                     /translation="MKAKIDPSRIISGTFGECWIDGRQMMAVKGLEAKVELEKEEVKV
                     CGQLMVGQKLIGASGKGSVNLFKVSSLFGEYVESMLNEGKAFKFTIISKLQDPDSYGA
                     ERVALYGCMLDDLTLADWEAGKMGEIEVPFTFESFKYLDKITEK"
     gene            complement(11480..12535)
                     /locus_tag="KPB88_000453"
     CDS             complement(11480..12535)
                     /locus_tag="KPB88_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0052763.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEME
                     DIPENYSATNLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     EEEGDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDPGSTIEINLDAQRAWLKENHIDVSNMEEQQIKEYNTDTIVFLKA
                     KIKLIDAMEDVYLDISM"
     gene            complement(12536..12985)
                     /locus_tag="KPB88_000454"
     CDS             complement(12536..12985)
                     /locus_tag="KPB88_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052764.1"
                     /translation="MLTNRDILIAVNEILKNEFIDIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKILNTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLTIE
                     NISPSIVKDSVGHKLTFLMTISYFEEVYFSKETYVTMENVDLKIGSD"
     gene            complement(12994..13350)
                     /locus_tag="KPB88_000455"
     CDS             complement(12994..13350)
                     /locus_tag="KPB88_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0052765.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTNKTGQELAENIRD
                     ETPVRTEKLKNGWEESQPEYDTYIVEDKVEYATLVNDGHATKNGGIIPGSFMVEKGIE
                     KTKENMTSAKANLFDW"
     gene            complement(13350..13697)
                     /locus_tag="KPB88_000456"
     CDS             complement(13350..13697)
                     /locus_tag="KPB88_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052766.1"
                     /translation="MTEADILEMTYLDKMTIIRKSKGWNQETCSNDFSNIIIAENIPC
                     AISRKEEAIISGDIGAIVINRKLFCRPEVDIKTGDTINATYSHGEEAVFKVSKANIYP
                     SHMEIPITEAERI"
     gene            complement(13709..14089)
                     /locus_tag="KPB88_000457"
     CDS             complement(13709..14089)
                     /locus_tag="KPB88_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG0052767.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVNMY"
     gene            complement(14073..14345)
                     /locus_tag="KPB88_000458"
     CDS             complement(14073..14345)
                     /locus_tag="KPB88_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052768.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            complement(14359..15282)
                     /locus_tag="KPB88_000459"
     CDS             complement(14359..15282)
                     /locus_tag="KPB88_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052769.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            complement(15311..15913)
                     /locus_tag="KPB88_000460"
     CDS             complement(15311..15913)
                     /locus_tag="KPB88_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBG0052770.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            complement(15939..16265)
                     /locus_tag="KPB88_000461"
     CDS             complement(15939..16265)
                     /locus_tag="KPB88_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052771.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            complement(16324..17085)
                     /locus_tag="KPB88_000462"
     CDS             complement(16324..17085)
                     /locus_tag="KPB88_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0052772.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            complement(17063..18340)
                     /locus_tag="KPB88_000463"
     CDS             complement(17063..18340)
                     /locus_tag="KPB88_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0052773.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            complement(18353..18556)
                     /locus_tag="KPB88_000464"
     CDS             complement(18353..18556)
                     /locus_tag="KPB88_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052774.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            complement(18557..20296)
                     /locus_tag="KPB88_000465"
     CDS             complement(18557..20296)
                     /locus_tag="KPB88_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052775.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            complement(20852..21310)
                     /locus_tag="KPB88_000466"
     CDS             complement(20852..21310)
                     /locus_tag="KPB88_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052776.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            complement(21525..22169)
                     /locus_tag="KPB88_000467"
     CDS             complement(21525..22169)
                     /locus_tag="KPB88_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0052777.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDIKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAEKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            complement(22173..22718)
                     /locus_tag="KPB88_000468"
     CDS             complement(22173..22718)
                     /locus_tag="KPB88_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052778.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDSVM
                     RETHSMYGRECSLDFIKKKQEKLLKLKKKIKKNYDSNINKIDSYLKILEESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            complement(22862..23305)
                     /locus_tag="KPB88_000469"
     CDS             complement(22862..23305)
                     /locus_tag="KPB88_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBG0052779.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            complement(23413..23670)
                     /locus_tag="KPB88_000470"
     CDS             complement(23413..23670)
                     /locus_tag="KPB88_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052780.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPKE"
     gene            complement(23690..24175)
                     /locus_tag="KPB88_000471"
     CDS             complement(23690..24175)
                     /locus_tag="KPB88_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052781.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKEAIDTGMTKDIIAEG
                     LDVCQTILTMFQILELEDYIAEGIEIHNKKLNGRGWDLKEIGDGILNLIKNSMINELI
                     NMNNKLKCVDEKEFNSKIQFLDVNKLWDLCVPLVNLSAMKGINENIDEMKMGEFLKNI
                     N"
     gene            complement(24248..24577)
                     /locus_tag="KPB88_000472"
     CDS             complement(24248..24577)
                     /locus_tag="KPB88_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052782.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSVGKDVCDKCYD
                     RLFGSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQDCLGGCLEGRKKWL
                     ESEEGQI"
     gene            complement(24580..24738)
                     /locus_tag="KPB88_000473"
     CDS             complement(24580..24738)
                     /locus_tag="KPB88_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052783.1"
                     /translation="MKTKIIGGRENECPICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            complement(24767..25351)
                     /locus_tag="KPB88_000474"
     CDS             complement(24767..25351)
                     /locus_tag="KPB88_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0052784.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            complement(25329..25700)
                     /locus_tag="KPB88_000475"
     CDS             complement(25329..25700)
                     /locus_tag="KPB88_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0052785.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEIGFQVLDDEDIPF"
     gene            complement(25697..25879)
                     /locus_tag="KPB88_000476"
     CDS             complement(25697..25879)
                     /locus_tag="KPB88_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052786.1"
                     /translation="MKSISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            complement(25937..26641)
                     /locus_tag="KPB88_000477"
     CDS             complement(25937..26641)
                     /locus_tag="KPB88_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052787.1"
                     /translation="MIRNAIEYLVKLGKRETVCVDGFKYTTDALEIIEEPKANELEIT
                     TLSGLVDYIKSGIDHEEDELLLIQVLTPRKVLLKSALRKNRDRETYIECVALLPDKRF
                     DRPLDLDTFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSTGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            complement(26693..27079)
                     /locus_tag="KPB88_000478"
     CDS             complement(26693..27079)
                     /locus_tag="KPB88_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052788.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSASVIDFRNASSK"
     gene            complement(27453..28784)
                     /locus_tag="KPB88_000479"
     CDS             complement(27453..28784)
                     /locus_tag="KPB88_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0052789.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDVGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIVDINDLKALVTTSSSINGNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAEAIANKEKAFLYSG
                     ELPSFMAMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRLFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVIDEEEEADDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQAEIPEDVF"
     gene            complement(28806..29123)
                     /locus_tag="KPB88_000480"
     CDS             complement(28806..29123)
                     /locus_tag="KPB88_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052790.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            29261..29449
                     /locus_tag="KPB88_000481"
     CDS             29261..29449
                     /locus_tag="KPB88_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052791.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            complement(29442..29702)
                     /locus_tag="KPB88_000482"
     CDS             complement(29442..29702)
                     /locus_tag="KPB88_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052792.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            29865..30077
                     /locus_tag="KPB88_000483"
     CDS             29865..30077
                     /locus_tag="KPB88_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052793.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            complement(30096..30314)
                     /locus_tag="KPB88_000484"
     CDS             complement(30096..30314)
                     /locus_tag="KPB88_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052794.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(30340..30525)
                     /locus_tag="KPB88_000485"
     CDS             complement(30340..30525)
                     /locus_tag="KPB88_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052795.1"
                     /translation="MNEKEEAVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            complement(30554..31285)
                     /locus_tag="KPB88_000486"
     CDS             complement(30554..31285)
                     /locus_tag="KPB88_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052796.1"
                     /translation="MDLLKDARKREWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSERQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHALPVVHEVHSKKTYNKKTYIKSNTTPQNEPKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIVNGKGSRYKSHYRALLTWG
                     NADTSKGILQPVTKAKNPLSGFKEL"
     gene            complement(31315..31533)
                     /locus_tag="KPB88_000487"
     CDS             complement(31315..31533)
                     /locus_tag="KPB88_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052797.1"
                     /translation="MNDEMITKMFSLNKTYKMTSIRLNLEVKGVITHIDKNEIVLEYY
                     DNELECITTKILTIDDIKYNDYNLKPLH"
     gene            complement(31550..31726)
                     /locus_tag="KPB88_000488"
     CDS             complement(31550..31726)
                     /locus_tag="KPB88_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052798.1"
                     /translation="MKKVDTKKISKSIKESTEFGILYSSLKDKQRDRVLGIMEGMKIA
                     NELSENNFMDIKHS"
     gene            31885..32256
                     /locus_tag="KPB88_000489"
     CDS             31885..32256
                     /locus_tag="KPB88_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052799.1"
                     /translation="MENIKDESVLKTLREALGLNQSELANILKMTPSAISKIEKNGKP
                     LSDRISSQLSKEFGVREEWLKNKEGKMFADSSDDDLVAEIAANIINSDDKFMKNVIIA
                     FNKLTEDQRNFLINFMKELDK"
     gene            complement(32840..33355)
                     /locus_tag="KPB88_000490"
     CDS             complement(32840..33355)
                     /locus_tag="KPB88_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0052800.1"
                     /translation="MSKMIQCKSCSKEIASNAKSCPNCGAKNKKPFYKRGWFIVVVVL
                     VLLYAIGTSGSGTDDTAESNDDGTSKTASVDKPEEPKKEKYEIVGDITSETDSMATYI
                     KGVIKNNSGHDISYAQVTFTLYDKDGNQLGTAMDNINDLKKDGTWKFKAMGMNTDGEI
                     ASYELSEITGF"
     gene            33729..34112
                     /locus_tag="KPB88_000491"
     CDS             33729..34112
                     /locus_tag="KPB88_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0052801.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALVEKEDYLKVETKSFFSFFHKNSFESLLSAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            complement(34261..34950)
                     /locus_tag="KPB88_000492"
     CDS             complement(34261..34950)
                     /locus_tag="KPB88_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0052802.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            35370..36557
                     /locus_tag="KPB88_000493"
     CDS             35370..36557
                     /locus_tag="KPB88_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBG0052803.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            36577..37377
                     /locus_tag="KPB88_000494"
     CDS             36577..37377
                     /locus_tag="KPB88_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0052804.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            37397..38131
                     /locus_tag="KPB88_000495"
     CDS             37397..38131
                     /locus_tag="KPB88_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0052805.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            38251..38403
                     /locus_tag="KPB88_000496"
     CDS             38251..38403
                     /locus_tag="KPB88_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052806.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            38791..39093
                     /locus_tag="KPB88_000497"
     CDS             38791..39093
                     /locus_tag="KPB88_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052807.1"
                     /translation="MEVNLHTGKDEFFKNHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            39128..39586
                     /locus_tag="KPB88_000498"
     CDS             39128..39586
                     /locus_tag="KPB88_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052808.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSIVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGEINVYKAGKIVSSQLVG
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            39816..40019
                     /locus_tag="KPB88_000499"
     CDS             39816..40019
                     /locus_tag="KPB88_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052809.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            40038..40943
                     /locus_tag="KPB88_000500"
     CDS             40038..40943
                     /locus_tag="KPB88_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBG0052810.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFEKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSVNVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYIDK
                     GFYLNGEKQDIKKNIDASVGLFKQIYNELKLNYPISTSAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            41025..41540
                     /locus_tag="KPB88_000501"
     CDS             41025..41540
                     /locus_tag="KPB88_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052811.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRVGNRFLEDNKSYDDKYQIDSINSKIDDDIKYLSNEIDNIKSVIEWFKTK
                     DDKCHIDSIKENDISIDLPDQSIKRTTIRVNNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(41551..41886)
                     /locus_tag="KPB88_000502"
     CDS             complement(41551..41886)
                     /locus_tag="KPB88_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0052812.1"
                     /translation="MLIQSLKRGELIVMKIFWKRNSRISRKEIIRICNEKHKWQKSTT
                     KILLKRLVKKRVLLKRIKIFHIYYIPIVTEKEYFKYKINDLEPDYLDGYFIRLMSTVH
                     KFKNLDEED"
     gene            complement(42220..42989)
                     /locus_tag="KPB88_000503"
                     /pseudo
     CDS             complement(42220..42989)
                     /locus_tag="KPB88_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(43048..>43580)
                     /locus_tag="KPB88_000504"
     CDS             complement(43048..>43580)
                     /locus_tag="KPB88_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0052813.1"
                     /translation="GSEVLYYSNKGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSK
                     PTAVLIESFFCDNKEDYDKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDG
                     EVDKIPATVVGWGYNDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEHYTMIK
                     GNDRFDTLYKALDFIK"
BASE COUNT        13983 a         7169 c         5156 g        17272 t
ORIGIN      
        1 aaaccagcca cctccaccgc cttcgccagt tgaatcttta gcagaacacc cttttccaaa
       61 acttccgttt tctgtgctta cacgaccaat accaccttga tattgagtac cgccgggacg
      121 atgtctgtcg ttagcgctat acccagtacc tcctgctaat cctcctcctg ccccaccagt
      181 ataaggatgg tatgaaccac cgccaccacc tgcgacaatt atacgagata gcaaaccttg
      241 ctcattatcc caagcaccac caacgagcct tatatcagta gcaccaccac cgtacataga
      301 ataataagta cccataacct gttgatttaa gtaaccttta ccgccaccat taaaaccact
      361 tttagtgtta ttactcgtag atgaagaagc aaaaccactt tcgccgacgt aaacatataa
      421 tgtagtttgt ttttttaatg taatttcacc tttagaatat ccgcctttag catcagtata
      481 ccaggaagaa ttattgatac ctccagaaga accccaacat tcaaatttat atttaccagg
      541 tttcaatata acactttgtg gcgaaccatt ataaccaaaa ttccattcag tctgcatttt
      601 ctcactctcc tctctaacaa taagttatta actcatttac actagttgca atattagata
      661 aaccaccatt taccttttct tctagattaa caaatctatc ttcgattttc ttagacgaat
      721 aagtagtcat ttcagatact ctgttatcat ctacagttgc attaataaaa tgagtttctg
      781 catttccatt tatcacataa acgtttaatt ctgaccttgt ttcacttcta atttctatag
      841 aattatcatc gataatttta aagtttgtaa ctacattttc ttttgtagta gcatctataa
      901 tatttacaac tattctctgt gttaataaac tatgtgttac agttgatttg aatccacttt
      961 ctgcatcctc aacccaatcg tcaattgtta ttgtttgagt agatgccaca ttagaaccac
     1021 ctgcgattaa ttggtcaatt ttaatatttt gtttctcatt ttctgtgtca attctagtgt
     1081 ttaactctgt tttagtagtt tctatgttgc ttgttaattc tgttttagtt gtatcaattt
     1141 tagcgtctaa atcttgtata tctcttaatg ttgctagcac aacagttggg tctattttta
     1201 aatttatact tgctgtatta gacacagcta atataatttt aattaatatt tcttttactg
     1261 ttcccgaatc tggagcaggt ttataagttt cgggatagct agatactgcg agaagttggt
     1321 ctttagagtc gaatactcca atctccctaa ttgtaaagct tccagtatct ccagttatag
     1381 ttttttggat aactacccaa ttaggatttt tttcgtctgc ttgaacatgc tctaatgtgc
     1441 tttcccacac tacatttttg agttctgttt gagtttctgt tggaatatat gcacttccac
     1501 caccatcccc aacttttatt tttgcaaaat ccactctagt tccaagtgct gtagcgtttg
     1561 ctatagccgc cttgcctatt tctgtaagta atgtataata ttgtgcttgt gccaattata
     1621 tcacctcctg tgggtataat gttacccttt cagcactttt attgtttcca ctagctagta
     1681 ttatttctcc aaaacattct atatttttag gtgtataagg gtatatagta actgtttctc
     1741 ctgtactgct cattgctcct gcataaagtc cattttcatt aaataaaatc atttcaaatt
     1801 tatgttcaag gtgtgcaggt tttatttctt ctattttctt atctaactct aaaatagtgt
     1861 tataactaca atcatttgtt ataaaactaa gtgtaaaact aaatagatta ctaaatactt
     1921 ctacatcaac attggtcttt gtgtaagctt ccgagatagc ttttataacc tctattgttg
     1981 ttgtaccctt acctctcatc tttgctttta tattgcttct tctagtatcg aagtctaact
     2041 tataatttac ttttatgctt aaaaggttct cccaatcatc aagtccccaa gttgctgtat
     2101 ctataaaaaa ctgttctaat aaatcatctt tttcatcaat tagcgttaaa agttcatttt
     2161 caagtgcttc ttggatttgt atgtcaattt cattgtttgc aaaacttggc aggtaatcaa
     2221 ttaacttcaa tttaactcac ctcgatatca aatatagcac ttgaaacagc aggagctttt
     2281 tcttcatcaa ctattatgtt tttaacatca tcatttacaa ggagattttt tatatcatga
     2341 acaccttcta tgcttgctaa caaactcatt atttttatat aaataatctc tcttgaattt
     2401 tctattaaat aagaatttat aatatctaaa aacacaactt ttatagattc aatatcatat
     2461 ccattttcaa gtgttaaagt tgcactaata ttaatatcaa aagtacttgg tgtaacaatt
     2521 gtaatagtag gtccaatagg tttttcttct tctatatgtt caatacatct ttgcaatact
     2581 tcattatcaa ctgattgatt attttgacca taaattagaa tcttaattgt acctggtcca
     2641 tcccaacgag gaattacttt tgcgttataa acaccttcaa cctctaaagc ccaagactgg
     2701 taatgtgctt tatttccact tgtcgcttga tttttttgta ttttatagaa cctttctttt
     2761 aattcttcat cagtttctat ttctgttcca ccttttaggt caagttcatt ataaatttta
     2821 gttactccat ttatttcttc tataagttta aattctgtgt tagctgataa attatactta
     2881 atgccaacct ctaaagcctg tataaggctt acatttaact tactatcttc atctattgta
     2941 acatctttaa taactacaaa tagtaaatca ctatgagata ctattgttcc atttggtatt
     3001 ttagttccta ttttgccctc aaatattacc tcacctattg cttctgtgcc taactttcta
     3061 tatacaccaa attcattgac tcttctatca agaaaatcgt caaagttatc ttgaataaaa
     3121 actctcttat gtatataaga aagttctata tataattgtg caagttctga atttattgga
     3181 gaaactatat tataaagaga agatccttgg tttttataaa gagggagatt tatgttattt
     3241 aaagttctat tatttaaggt actaaatgat tggccactat acaaaagtaa tctcctcctc
     3301 tccataaatt gtttttacat ttaaacttat agataaatta tcatctttaa attctgcatt
     3361 tgttacattg acttctaata tatatggatt agttaataga gcttcttgta tatacctttt
     3421 tgcctcactt tcagtaagac ctttagtata cttttgccct attagatttt ttatatctgt
     3481 tccataatcc caatcatata ttaaataaac atacttatct gtttttattg ttttgtaaat
     3541 ccatacttta attgcttcat ttctttcaac tattttaaag tctccatttt caattacctt
     3601 ttcatcttta tcaaaatccc aagcaaattc tttaaaaata ggtaattctt cattgtctgg
     3661 agaaatataa tcttctggaa cacccatgaa agggaatata gtattattca tctagactca
     3721 ccaatttact tacaacagca aatttttcac ctattttaaa cattattact gtgtttccag
     3781 attcaaaagt atctataaag ggatttttta tttcatgttt atgttcttga cttgtttctg
     3841 tatcaaataa ctctatctgt ctatcaagca tccaactatc taccaagata tcttcttttt
     3901 ctaatatgat gttatttatc tctattttta aatctggtaa tttactttta atttttccaa
     3961 taaaaaaaga aggttcatta tgaaatttac cttcttgtct cattattcct ataaattcat
     4021 tgattggatc tgccacgata tcacctcttt tacaaaactc ttctagctgt attaaaatct
     4081 tttctattac ttaatttact tattttgact acatctcctg tttgtggcgc atgtaaaaat
     4141 tctccattac ctacatatag tccaacatgg ctgactggat tatggaaaaa taccaaatct
     4201 cctgcctgta aattattttt ctctactttc ttacctacct tagattgctg acttgaggtt
     4261 cttggtaaat taacattaac cttcttaaag caatatacca ttagtccaga acaatcaaag
     4321 ctacttgctc cattcccacc ccatttgtat ggctttccaa gatgttttct tgcttctgaa
     4381 attactgttt ttgctttttc tgtcacgttt ccagaataac caccaattat aatcttccct
     4441 tttcttcttc caaagttatt ggcttcttca acatttccaa ataaaatatc tatatgatat
     4501 gttccatcct tttctataat tattgcaggc ccattatcat ttaccttata cgttccatct
     4561 cgacttgaaa cacctgtaac caattgtatt tcatctccat atctcataag aggatgtttg
     4621 tttaagaaat cttttgtata gtaagatttt tcatatgttc caaccatagg agcagcgcat
     4681 gtttttttag aaggatctag ttttttacct ctacaatctt tatctccacc ctcagatttt
     4741 ctaggacaat aagcagtaaa ttcagcagag tattctgttc ctccagtata ctctgaacca
     4801 tcttcttttt gttcatcttg accagcagat ttttcatcca ttatattctc aaaattaagc
     4861 tctagttcaa tttgataatc tccattttgc catgtatgct tatctgtatc tatataaaaa
     4921 agacctatta attttgtata tgagtctttt acttttacac ctctaccagt tacacaagtt
     4981 atatcaccat atccttttaa agagcaagtt ttttcgattc ctttaaactc tccatctatg
     5041 tctatagtac tgttttcttg ttgttgtatt accttttgca taattacccc aacgtcttta
     5101 aatatagagt catttatttt ctcactaatt ttattaccat actgatcaac aactagtact
     5161 ttatttttta cattctccat gctttcagaa aaatttgtat tgataagatt aaagccttct
     5221 tcaaatgtta tatttaaagt aactacaccc ttctcaataa cattaaattt atcaagatta
     5281 gactctatca tatacttttt tttagttgtc ttgcttgctt ctgtataaac gctcattata
     5341 gtatcatagc cagttacacc aataaacatt tttgtatatt taacgttagt tttaggtaaa
     5401 ttacctagtg ggaatttatt gtctacaaaa acctgctttg caatatcttc aactaattta
     5461 tctttaaaat tgtatgacac ttcgctttgt aaaagtaaaa agcccatatc tttagctgta
     5521 aaatctatat tattattgct agagtcttta gatctatcta ttatcattcc tctaaaaagt
     5581 tctttatcat caacatagaa acaaactgta cttgctactg gaatatctat ctgcctaaaa
     5641 ttaatatcac ttgcagattg aattatagaa aattctaaag ttcttgatgg tgatctataa
     5701 tcaccagacc aagtacactt atccactaaa tcagttacat tatatatatt tccgtttttt
     5761 atatgaactt gtattttgat tttattaatt ataaatcacc accattttaa ggaattatta
     5821 atatccaacc attttttatt acagatggat ctttaatctt atcttcattt gctttataaa
     5881 tcttctccca caaatctcca ttgccataat atttttttgc tatcttaaaa agggtatctc
     5941 cttcaacaac cttatgtgtt ttttgcttag tatcaaatcc ttttgttagt ggaacatcct
     6001 tttcagaaga taatttttca tcattattaa tgtttacttt ggatatttgt atccttctat
     6061 attcttttag acttaaggta aaataaacat ctcttgaata atcttgttct ctataattaa
     6121 aatctgcaat tatacattca aagtttatat tagtttctgt aatgataaat cttaatatat
     6181 atccttcttt catccaactt tctattaaat ttacacaatc atatggtttt ggaaatccat
     6241 tataattaca gaaactatat tcttgattag ggaaaaaaga ggatagttct atagttttaa
     6301 gccctaaccc tccaaatact gcaatatctc caacacttaa aatattagat gtatttattg
     6361 ttgcacttcc atttatctca aaagagggtg gaataacagg aaacctaaat gtattatttg
     6421 cttgtcttaa ccatatttcc attaaactcc tcctaaaata aaagacactt atttcaataa
     6481 gtgtctttta agataattac tataaatcaa atactgtata ttttcctaca ttttcataag
     6541 caatttcaat tgtttttggc tttgtatctt ttgtaagttc aaatgctatc tcacctttta
     6601 atgtatctcc tggatgtatt gtgtcaaata gtaattctcc atttaattct tgattattta
     6661 ttgcatttcc aactgtatta tcatccgttt ttagtatgta gttttgaagg aaatcacttt
     6721 caagatctgt atctcctgta ttggtgattt caatctcaac tgcatataca ttactatctg
     6781 attctagttt tctaacattt ttaacagata cctttgctcc gtccaaatca acaacttcgc
     6841 ctattttatg atttttaatt tcctctgtat tttgcttagg tttactatct ccattttttg
     6901 aatcatttgt gctgcatcca actaaactta aagataatac aattaatatt gctaatgctg
     6961 gtattttttt attcataaca taaccccctg taaattatat actacaatta tagcacgata
     7021 atttacaaaa cgcctccaac ttttgcaaga gatatttttt tgtttatttt tcttacaatt
     7081 ttatctatgt cagcctcttc tcttacaatt attgtatcag ccaatttatc aagaaataaa
     7141 cttccatttg aactatttct tttgtattga ttggcttctt gtttggttaa aagtttctct
     7201 ccttcatgtg ccctaattaa ataatcattt ctaggaactc tatttatacc aaatgctttt
     7261 cttggacttt tgccagcagc atctgcacct tttccaattt gtaaactagg acctttttta
     7321 aatatttcta ttgttccctt aattggatgt ttaaagaaat ctttaagttt attccaagct
     7381 tcttttaccc ctttaacttt atctttaaaa aatgagtcag caagatctat tacaggttcc
     7441 agtacaccac taactagatc aacaaggcca ttccaaattg attttattac acctactcca
     7501 ctatcaaata tttgtttaag tccatccata gtttgatcag catttcctgt tataagcccc
     7561 ataataacat taataattcc agatataaaa gacataattc catttatgat tccagatact
     7621 gttgttatta ttgcggttaa tgtatttaaa acccatgtaa atgctaaaat tattccgcca
     7681 acaacaccag ctattgcaat tcctatagat ggtaaaagtt gttgtcctat ttgtgatagg
     7741 aaaggcataa atccttgaaa ccaagcttta acaggagcaa gtgcttgagt taaactttgg
     7801 aaagaaagtt taattccatc agtagacttt ttaacactat ccattggttg gcttactttg
     7861 tttactgtat ctccaacctg tttggctgga gtaaaaaaat cagtaatcgc ttttttaacc
     7921 ttatcaaaac actctttcaa attatctaaa tgaggtttta aaggttcaaa tgcttttact
     7981 aaattatcta tatttgtttt aatatttcca gctaatatgt tctttaattc attaaattta
     8041 tcctttattg aagttattga ctccttaaac ttatccttaa aatttactcc agaatcctct
     8101 aggaccttta atgcaggttt aacatcattt aatattttgt tttgcagttc tgtaaattta
     8161 gtttttccag attcaactcc ttgacttata gaatctgtca taggtttaat aaaagtttta
     8221 aaatcactaa atgaaccttt tagatttgct attgtttgtt ttaatccacc tgtatttggt
     8281 gtagatttcc cagctgcatc agctgcttta cttcctggaa tgtttacatt tgttttagta
     8341 gatattttgg cagttgcttc aattggattt tctaagaatg ttcttaaatc tttccactta
     8401 tctgtaagcc atttagcttt atctttaaat aagctgtcag ctatatcagc tatagcttgt
     8461 ataggagcag ttacaaaatc tatgaaacct ttccaaattg attttataat ttttactcca
     8521 ccatcaaata tttgtttaac tccattcatc atctttttac tatcgcctgt gacaatacct
     8581 ataattaaat taaatactcc atttaaaata gatgctagac catttaatac attagttata
     8641 gtatcaacta cagctttaaa ggcagttgcc agagtagata aaatcaaaac taattttgct
     8701 atatgaaaac ctattacgcc aattaaaaca ggtcctagta ccttcattat tacacttcct
     8761 aagtttaaaa tagacgcaaa tagtggacca aatgctgata acaactcttt aaatttttcc
     8821 cctaattgtt taagtgcagg catacaaaaa tttactatag gttttgcaaa ttctgtaaat
     8881 gattttacta atgaattaac cttatttcta aatacttcag atttagcata agcaatagta
     8941 aatgctactg ccaatgcaat tacagctgtt atagtccatc ctacaggacc cattacagct
     9001 aaaaatactg cacttaatgg tttcatgcct attacaaaag ctctgaaagc aacttttgct
     9061 cttaatatta ttggaacaaa aaagccaaat acacctatag cttttgaaac aattaaagac
     9121 acagccccta tagttaataa aagtactcca aatgctccaa ctgaaagcat tacattagtt
     9181 atgactcttt ttattggttc acttagatta ttaaaccaaa cagtcatttt agttaaatta
     9241 tcaactacag atgctattgc tggctttagt tgatagtaaa tagtaattcc agtttcttca
     9301 agggctgatt ttaaggttgc aagtccaccc ttagtattat ccttcattac atcagacatt
     9361 gtttttaaag caccttttga attatctatt tttttcttta aactatcata ttcatttccc
     9421 acaccatcaa gtaacttttg taacgttttt aattgagtct ttccaccaat agcagataag
     9481 taataatttt tctgctcatc agtcatacct ttagttttat ttgcaacctc tttaagtaca
     9541 tttgacatac ctttaaattt accttgatta tcaaatgcac ttacacctaa ctttttcatt
     9601 gccttacctg cttgacctgc acctgttgta agatttacca tgatagagtt taaagcatga
     9661 cctgcatctg tacccttaac tcctctattt gcaagtattc ccatgatagc agttgcttct
     9721 gaaagtggaa cttttaaatt gctaaatgta ccacctgcaa cattaaacgc ttccatcaac
     9781 tgttgtatac ttgtatttga tttagtagat gcatttgcaa ctttatctaa atactcactt
     9841 aaacctgcac cagctagtac ttttccactt ttactgacct tatcaacttc taagcacatg
     9901 gaactcatgc tatcagttac taaatctgat gctagaccga gatccataga ggctgcttca
     9961 gataaattaa gcactggcat taaggcatgc attgactttt gaacatccca tccagcaagc
    10021 cccatatact gaagtgcatt agcagaatca gcagcagttt tagttgtagc ttttccagca
    10081 tctctagcag ccttagacag agcttcaaaa tctcctggtt ttaaggaatt tccaaatgta
    10141 gccttaactt gagacatagc gctttcaaat tctgttccaa ctttagttgc agctgcaaat
    10201 gcgccaccaa ctccagcagt gataagacct cctgctttta aggcgctagt tccaacactt
    10261 tttaaattac tttgaacatt atttaatgct cttgatgctt gggaagatga atcatgtaga
    10321 gccctagtat ttgctgctac tctttttaat gttgaacttg cttcatctct catttttatt
    10381 attgcttgta ggacctttgc attagaactc atatactaaa acaacctccc agcaccagat
    10441 tttatttctt cttttctttc atccaattct tgatccataa atacactaac aatttctttt
    10501 tcgcctctta gtatacgata agaaacggat ggcattatac ctttatactt aaataataag
    10561 tacatcaagt taacttctcc atccgtttta attagttttt tatatcttta gctctttctt
    10621 tttgtctttt tttatctcct tcagcatcta tgccatttac tttattaact tcctcatata
    10681 aattatcaat ttctcccgct aagagtaatt tttttagaag gtctttgggg gttgccaagc
    10741 ccagattttc tacaacttcc atatttctaa acattggaca tgactcaagt attgtattta
    10801 ccttaagttc ataaacatta atattttcta aattaccact atctacatcc attgaattca
    10861 ttcttatctc atcaaaacgc tctggatcaa tagcattaca ttcaatttct aagatatcat
    10921 ctaatttttt gcaatataat tcaaatatac tagacggcat tttcaattta cctgcatcca
    10981 tatttaataa tttttctact gtattcatat aataaaaatc ctcctatttt tctgtaattt
    11041 tatctaaata tttaaagctt tcaaaagtaa atggaacttc tatttctccc atttttcctg
    11101 cttcccaatc tgctaaagtt aagtcatcaa gcatacaacc ataaagagca actctttcag
    11161 ctccatatga atctgggtct tgtaatttac ttattatagt aaatttgaat gcttttcctt
    11221 cgtttaacat actttcaaca tattctccaa atagggaact cactttaaat aaatttacag
    11281 agccctttcc agatgctcca atgagttttt gtccaaccat caattgtcca caaactttaa
    11341 cctcttcttt ttctaattca acttttgcct ctaagccttt tacagccatc atttgcctac
    11401 catctatcca acattctcca aatgtaccag atattattct acttgggtct attttagctt
    11461 tcaaatttta acctccaaac tacatagaga tgtctaaata cacatcttcc atggcatcta
    11521 ttaatttaat ttttgctttt aaaaatacaa ttgtatctgt gttatattct tttatttgtt
    11581 gttcttccat attagataca tcaatatgat tttcttttaa ccatgctctt tgagcatcta
    11641 aattaatttc tattgttgaa cctgggtcaa ttaaaccatc cttttctaat tcttgaagat
    11701 ataattgtat tgcaacaata agtaaacatt tattatcata agtgttttgg gtttttccta
    11761 tataattttt tacaattatt tttttaatat cgttatgaat ttgatctagt gtatcaacca
    11821 acttaatctt tttgaatata tcaccttttg aatcagtaat tgttgtaaaa gatgtaactc
    11881 ctctagcgat cactatagct ccgctttctt ttattaaaat tagctctcca ttatttatcc
    11941 ttgtatttgc ttcagctctt gttaatttag gtatattagt gacttctggt atttctgcat
    12001 atgttacaga ttgagttgat ggagttcctg caataaaacc tgctattctt ggtagaaatt
    12061 catttgaagt gtatttttta ccttcaactt caatgtcagt tgctgtaaag tttattatac
    12121 cttcataatc tgctgaatta cttgcagtaa tagctttaac ctttatttta ttatcttctc
    12181 tcattttctt aatccacgtt ttgattttag gtaaatctcc ttcttcttca tctggcatac
    12241 aaagataatt aaattcacaa gtttctagaa aatctagtgc atcatctatt gattcatctg
    12301 tatctaatgt ataaactaat actttatttg gaggatatac atttcccttc atagccattt
    12361 ttatatagtc taaattagtg gcagaataat tttctggaat gtcctccatt tcttttatct
    12421 caactagccc tatatttttt gtatctttta aaataagtgc tacaatacct ccagaacgtt
    12481 taacaaaagt ttttcctgct tggataaaag aaatattaat ttctggtaaa cccatttaat
    12541 cacttcctat ctttaaatct acattttcca tagttacata tgtttcttta ctaaagtaaa
    12601 cttcttcaaa ataagatata gtcattagaa aagttagttt atgccccaca gaatctttta
    12661 caattgatgg agaaatattt tctatagtta aactcctatc tttaactcta atgtttctat
    12721 taaatatttt ttctaatcta ctttgtatgt catatagatt taatttattt tttctagctt
    12781 tttggtaata ttcaatatct atgaatatct ttttagtatt caaaatttta tttgcaatat
    12841 tgttagaaac aggtaaaagt tgcacaaaaa aacaagcctc ctcaaagcct tgtatattat
    12901 cctcattaat aaaaatatct atatctatga actcattttt taatatttca ttaactgcta
    12961 ttagaatgtc tctgtttgta agcaactcac cacctaccaa tcaaataaat ttgcttttgc
    13021 acttgtcata ttttcttttg ttttttcaat tcccttttca accataaaag aaccaggaat
    13081 aatgccacca tttttagttg catggccatc atttactaaa gttgcatatt caaccttgtc
    13141 ttcaacaata taagtatcat attctggctg actttcttcc catccatttt ttaacttttc
    13201 agttctcaca ggcgtttcat ctcttatatt ttctgctagt tcttgcccag ttttatttgt
    13261 aaacttcttt atttttctat taaagttaga agcatttaga cctaaatcta aggctaatgc
    13321 ttcaaagact cctaaattat ctattcccat tatattctct ccgcttctgt aataggtatt
    13381 tccatatggc ttggatatat attcgcttta gaaaccttaa atacagcttc ttctccatga
    13441 ctataagttg catttatagt gtcgcctgtt tttatatcta cttctggtct acaaaataac
    13501 tttctattta tgactatagc tccaatatcg ccgcttataa tggcctcttc ttttcttgaa
    13561 attgcacatg ggatattctc agctataata atattactaa aatcattaga acatgtttct
    13621 tgattccaac cttttgactt tctgatgata gtcattttat caagataggt catttctaat
    13681 atatctgctt ctgtcatttg atcacccctt agtacatatt aacaatacaa aatggactta
    13741 gaaatttttt atcagtatca gttaaaaaag atgatgaact gatgctattt tcagcagttt
    13801 gtaatgttaa agcattatat tctatcttag tgtcacctct agttatagct tttaagcttt
    13861 tttctagatt atcaagtcca ctatttttct taagttcttc aaatcttgaa gataatattg
    13921 atattacttt ttcttctaca aacccttcta gtgctatact tagttcctgc ctagaacaat
    13981 attctaaaac catctcactt attttattta tataaaggat tattaatttg tcatatctat
    14041 cattttctat tcctaaaact aatttaacat tatcaagcat tgtactcatc tagtagttga
    14101 attaattcct ctttagtttt agttgaatat ccctttatat tcttgccttt agcaatttcc
    14161 ataagctctg gtttagatag attaaataaa tcattagagg ttgatttaac tttttcttct
    14221 tttttctcaa tattctcttt agtcacttct tctgaatata ttttaaatcc ttttgaaatc
    14281 aatttatctc tcgaattttc atcttcaaca actttaatta tgttattttt tatcaatgca
    14341 aacatttaaa acaactcctt attcttgtgc agctttagct tgtctcacag atgctctaca
    14401 aagttttaaa tattcatcca atatccacaa atcgtgatat tttctatagt ctatttgata
    14461 agcatctgct gtttggtttt gatctggagt aaatattctt ggcttatcag tcttagatac
    14521 tgcaattgga gcagatgctg gagttattaa ccagtttata tctaaagcct ttgctccttc
    14581 agtaaaccca ccctctgttt gtccagatgt tttaccatct ctaaatacgt attctgtttt
    14641 cattcttttt gatggaactt ttattatagg tacattgtct atagacttta caattctttt
    14701 tatatctcca tcgccaaatt caatatcatc aagttgagat gattgttcta atatgcttgc
    14761 aattatgcta gacatactaa taataagagg tatatctcca gataaatctt gtattgatgc
    14821 aagttgattt ttcaattcac ttaatattgt agttactgta gcttcgtatg aatattttac
    14881 acgctcatct actttagctg tttcagaaaa tatctttgag tatctatatg catctatttc
    14941 tggtataaca tgttgctttt gaaataatcc cattacagaa gatgcattta taacgaagtt
    15001 actttcattt acatccattg aatctaacat aaaagtccta cctctatcca tctccatttt
    15061 cattgtttcg tactcaagtg taactgcgcc ttgcgtatat cctttagatc tgtcataatc
    15121 acccaagcca tccattgtta cttttggtac ttttacctct cttcctccat tatatataac
    15181 ttgaccagca tttttctcca tccatccagt agttgagcca actgttacct gcatatctag
    15241 ttcttgttga aaaagtgtta catattgtaa tacattagcc atttataaaa tcatcctttc
    15301 tttttttata ctaaagacta tttagccctt gctttatttg ttttctaatt tctgatagcg
    15361 aacctgcact atctttgcca ggtggaacgt atgaaccatc tttaattctt tcatctacct
    15421 ttgactgcac atattgcttc attgaatttt caaatatctc tatatttgca tttgttttat
    15481 catcatcatc agataataga tattcaatta agtttgtagg tattttcttt tcaactaaaa
    15541 tatctttata tttagaaact ttttctgcgt actgcttttc tttttttagt ttgtctaggc
    15601 tttctcttaa ttcccttatt gctttttgct cttctgtttc atttgctcca gttcttttta
    15661 gaacttcagc acttataagt gtctgcatat cttttttctt aaagttttca agtgcttcgc
    15721 tgtgatattt atttttttca ttatctataa tagctttaaa gtttgcatca ttattaatta
    15781 attctctaaa ctcttctatt gtaagctttt tatcagatgc agatttgaat aaatctgttg
    15841 attgcagcaa tgaatctatt tctgcatcat cttctatttc ttttagtaat tccaacactt
    15901 ctttttttaa catgtataaa gttctccttt ttgaaaattt aattcaaaca ttctatttta
    15961 tccataacct ttactatttt aggaaattga attgcaaaga agtcaatcat ttcttcgttt
    16021 gtagcccaag aactacaact cttcaatcca ctttcactaa gaaatgcatg aattatttca
    16081 tgcctagcaa cttcattttt atatacactt aaatctttca atgaaccttc ttcagaattt
    16141 attttctcta taacaatttc cttaacagag tgatcacaat atccagctct ttcctttaat
    16201 agcacatctt cgctttcaag tttctctatc actagatatt ctgttcctaa tatttcaact
    16261 ttcatatttc acctcatatt ttttagacat aataaaaaca cctattaaat taattaatac
    16321 atgttaaaca ggtatatatt gacatctaca gtgacagtgt cttggcaaag agggcctatc
    16381 ttcattgaca taaaatatca ctccattatg catagaacaa tcactacatg tgcgagaatc
    16441 taaatctgca caatacatta cttttataat cccttgaacc ttgaaaaaat actcatttac
    16501 ttcactttga catctggcaa cttcattttc tattagtctg ctggtttgtc ctgcacctgt
    16561 tgaatattta tctcttattg ctttttctat cttatttaca gaagtttttc catttagaaa
    16621 actctttatt tcttttttta aggactcttc tatacctttt ttattcttcc agagtctatc
    16681 gctccaatgt tttccatcaa ccttattatt cactatctta tttataactt tattgtttat
    16741 attactaaca tcttttatct tcagtattac tcctaataaa attatcaaat ttgaatattt
    16801 attcttagat gaatcagata aaatagtttc tatatccttg acttcttcat cataactatt
    16861 agaatactca tttataattt tattaagttt tgaccttaga tttttcttat ctttattatt
    16921 aatatttaag tattcattaa taatatcgtg tgttagcaat atctttgcta tttctaataa
    16981 aagtttatcc ctattttctc tttgatactt ataaaatggt tttgtcctat tttcagcaga
    17041 tttatatata tcttcagtca atttagttat aatcttttga ttcatctaat aaaatctctc
    17101 cctctgaaac tatttcctgt tcttttttag ctttttctat ttcagcatga ggattatcaa
    17161 tgaaacttag ttgagataag ccagtttcag tagataattt atctccaagt tggcttaata
    17221 tatttgctac aactaaatca tcacgtggta tcttaggggt aaatttagcc ttaatatctt
    17281 tataatcata gtcaaaacca tattttttat taagccaaat gcacaataat ttgtacctat
    17341 taaataaagt atctgcaata ctatcattat tttggctaca aatccattct gtactgatta
    17401 atcttgctat aactgctaga cttgatgtat tactttgcat tttttcattg tgatttatat
    17461 gagatgtaat ttcatacata ttttctttta ttgaagatag tgtattttga acaaatgtat
    17521 cattcatatc tttaataagc cactctattt ttccatctcc attaacttga agtattccaa
    17581 gttctttcat ccttggcaaa tcatcttctt taatattaca tccagaaaag gttaaataag
    17641 catttctaaa atcactaatt tcattactta tatcacttaa attagtttca taagcatctt
    17701 gtaacccttt tatatcatta aatattgtat tttttcctag ttcttcactt cttttgcaaa
    17761 ttgatattgg aacatttcca tcaaatatat tgtttactgt aggactttca acttctttga
    17821 aatttgaatc aaaatgatat atatattctt tgtcatatac atctacatac gtgtcatctt
    17881 cgaaaccttt ggtaaactct ctaataaagc atactatttc tccattttca ttttcataat
    17941 gacagccatc tgttggcttg attatcttta cttgtatttc atcatctttt acatagtaaa
    18001 gttcatagca aagcccaaat aatagtgcat acctcaataa atctgagtca tgttttttac
    18061 tccaatggca agtatagtat tctaaatcat ttataatctt ctcatcacca gatctacttg
    18121 tataggtaat cttatttgca agagaataag cgacttcttc attaataaac ttttttaaga
    18181 aattagtgtt tatcttatta ttagatctct tggtaaccat tttatagttg ctaatagcat
    18241 ccgtttcacc tttataatac ttatacatag tatcatattc ggatttatta gagttccaat
    18301 cgtcatatat gtcttttatt aagtctaaat ctaattccat taacaacaca tcctatcctg
    18361 tagactctat aaattttaaa acttttattg gaatatcttg ctttgaaata taacttttac
    18421 tccttgctct atgagtatat gcaaaatatc ctttttcatc ttttcctatt ccaacattga
    18481 acttctttcc ttttttagtt aaagtttttt gaacttcttc aaactttgat ctattagcca
    18541 ataaattacc tcccaactaa aaacctaata attttctatc aaagatattt actgtctcag
    18601 aaacttttat gttgttaatt cgattaataa attccgatga tatatccgga gcatcatcat
    18661 gtagagagta tttttgtcct gtaaaatcaa gtatttgctg tataaattcc ttatcttctt
    18721 ctgcaaaaat catttggcct ttgcttaaaa caggtattaa agtagagatt ttatcatctt
    18781 tatttttctt ttgttgctca ttaatgattt ctatatctct ataactaaga acattatcat
    18841 tttttatttt aaactctaat tggttggcat ctgcgccatt aaatgtattt ttttctatat
    18901 aaatatgtgt tatatctgaa tacactttta ataaataaat catatggtct atatatttat
    18961 caaaatcagt tctagcattt attttagcta gttctgccaa tcttccatat agtaaaccat
    19021 tttctgattc acttccaaca agaaaagcgc tatagtcatg tttcgaccca cccgcagatg
    19081 ctggatcaca taacagcatt gtctttttaa aagtatgcga ttcaatttct tctctagttt
    19141 ctgttctaac agttttaaac catttttctc ctatagaatt gacatcattt tgcacttcct
    19201 gcttaaaaga tgttggattt tcataataac ttagagccat atctaaacaa tcccaaaaac
    19261 ttggccataa tatagggaat ttcatttctt tttcattttc ccagtaaaat tctttagcat
    19321 cttcaagatg atttgtattt ttaaaatcaa ataagatctc tttgaactta agccataaac
    19381 cagaattaaa taattcatct acattatcaa ctaaaactcc tttttcgcat ttaaacttcc
    19441 aagttggtaa attttttagt ctactataaa aacattcttt gtgttgtaag gttcctaatg
    19501 ctataaaagt tgttcctctt tttatagttt taccatttct tatgactggt ttttgagcag
    19561 catattttac atcatcagaa aatcttttaa attttttctc tctagcatct tctgttctaa
    19621 cattttcttc tgactgataa tcatcaagta tgataagatc tggacggtta ttattgtatt
    19681 ttcttcctct catgggtgat gaagatgata tagcttcaat aaaagtttta tttgttagct
    19741 ctagttgagt actattacaa atgtattttc tatcattatc atttaaaagg acaccaaatg
    19801 cttttttaat atactcattt tcaagtaatg cattttttat atcttttaca aatttctctg
    19861 ctgtagatcc tatgtcagag caaatcaaag tatatgtctt atgtttatag caatgcgacc
    19921 atacagttgt cgctaaatct ccaaatgcac ttttacctgt ccctcttgga taaattcttc
    19981 ctaattgctg tgaaccatct ccaattatag attcttgtat atcttcccat agctcatgat
    20041 gaactttagc tataggagca gcagcattat cttcttttgg taaatatgtg tcttgcataa
    20101 aatacataca gaaaaattct aggtttattt ttccaagttg ataagcgaga ccattaactc
    20161 cccaaagatt gtttgaattg gcttttataa tctcatttgc ttctttttca gcatctttta
    20221 tagatgctcc atttcttata aaagtctttg ctagataact aaatattaat tgtatatttc
    20281 tttctagatt atccactcgt atttacttcc tcttacttct tctatttttt taggctcagt
    20341 gagtaatgca attaataaaa taaacaatgt aaatttttct ctaataaaat caccctcttt
    20401 gaaatacatc tctggttttt aaattttgct agaaaatttc tagaactcat tttccataaa
    20461 aaataatttt ttcaaaatag aaggacaccc cctattaata aaaaaaaata aatatattaa
    20521 agtattttga attattttct tgcaagaaat atttttttga ttagaattat taattatctt
    20581 tcaactattc cttaaatatg tattttcggc atagttaaaa agaacctagc atttccaata
    20641 ggttcacata tcctataata atccacatca cttcactgta tgaaacttta attattaagt
    20701 ctcactaaat tttattaaat tagctataac taaagttaaa tacaatatat gatttaatta
    20761 ttaaagtaaa tatatatata atatatgttt tgctttaatt gatttcaaac ataattaaaa
    20821 catattcttg caggatttat ttcatatcta attacttctt gcttgttctt attcttatct
    20881 tagctatagc tgcttctatc tctgttgcat ccatacccat gccaacacta ttatcctctg
    20941 cttgtataac tgtacttgta ggagttccat atatacgatt tataagatac tgattggctg
    21001 ccaacataac cctcttatcg ctcttgtcac tagctaattc cttgatattg ttgatataac
    21061 tatctaaatc ctttaatatc attaaatttc cttggtttgt tagctcctgt ttccgtctgt
    21121 ccatttcttt cctaacatgc tctttattca tccaattata gattgttttt ctatttactc
    21181 caataattct ggcagtttct gttactgtag cacctgtaat caatagagtt accatattgc
    21241 actgatcttc tgtcaactct ttaccttttt catagctact atcgattatt tcaacttcat
    21301 tcacatccaa ttaatcacct ctttactcag cttctaaata aacatttaaa tatctacatc
    21361 cctataaatc ccttgaataa atgaccttta atatatattt tcagacattt taataaatct
    21421 atcctattct ttggaaattc tattattgag gctctttaca ttctaataat gtcctgttgt
    21481 tgaaaaacgt aacatattaa attttagata aaatacaaga tattttaatt tctttttctt
    21541 ttctttaact tattagtaaa tttagttatt gcttttactt cttcatcctt atctattcct
    21601 atataccttc tagtcatttc tattgtctta tgtcctagaa tctttcttat aagttctaat
    21661 gcataagtct tattttcttc ttcatcataa atatgccttg caaatgtctt tcttaaagag
    21721 tgtgttgcaa cattttcttt tattcctacc ttttctgctg ctccttttat tattcttcct
    21781 gcttgccttg tttggatagg cctattaaat ttcttcctct tgccactttt aaataaaaac
    21841 tcttctcctg gtttatcttg agtatagttt tctatagcac tctttattac atcagataag
    21901 aaaacttctc tatcatagtc tcttttttct tctataactc taatccagtc cttagacact
    21961 cttatctttt ttcctttttt atctaacttt atatctgtta catctgcaac tttaagtgct
    22021 aatatatctc ctattctaag tccagtacac attccaaaga gggctaataa gtaatttctt
    22081 tcgttccatt cctctagaaa attcaagagt ctaaatacat cctctatatc ttttattgga
    22141 ttaacttttt gtccactatg tgcttccatt cattaaaaac actccttatt accatgataa
    22201 ttttttcttt gcctatcttg caccaatatt tttaagtctt caactacatt agcaaaagga
    22261 tctgtgatta tagaggttat atcatttgta ctatattttc tttctcttgc ataagtattg
    22321 gtctttattc taaatcctaa gtcatttaca gtttcagtta cttttacaac attttctaac
    22381 tgcaaatata atttaaagac aaccaactca acgtgatcag actcatcaat tatactttcc
    22441 tctaaaattt tcagatatga gtctatctta ttaatattag aatcataatt tttcttaatc
    22501 ttctttttta gtttcaagag tttttcttgc tttttcttta taaaatctaa agaacattct
    22561 cttccataca tgctatgagt ttctctcatc acactgtcat aatactttaa attatctggt
    22621 acatatgcag cacttgaaat ttcttctgtt atatcacgtt taatcttcaa cacctctcta
    22681 gacaataaaa aagacttaga acgtaaattc caagccattt tttattaaat atatttttct
    22741 cacaatacaa tcttatcatg attaaaagac acaaaacaga cataaattgg tcacaaatcg
    22801 gacatttttt agacacaaaa cagacataaa tcggtttaaa atcgtcacaa atcggacatt
    22861 tttatagcaa tccttttatc ttatctataa tatcaaatct catttgctta cacttactcg
    22921 ctgaaaaacc aatcattctg ccaacatatt cccaagatgt cctgctactt gaaaagtatt
    22981 tatactgtac tatctttttt tcttcttcac ttaatatttt cattgcatta tctatttttt
    23041 ctattatcaa ctctttatgt ctcttctctt tttcttttaa ggcaattttc ttttcttttt
    23101 cttcagtctc tatttcaatt acacttttaa tatcatatgt cttgcttgtc ttttctttaa
    23161 tttcaactcc actacatcct ctgtagtcgt tttttatcat ctgcatatca atgtctattt
    23221 tttctatttc cgcttttatt tttttataat tatataactt accttctgca ctactaaaca
    23281 aattgtcttt tttgctagat tgcattacct cacactccta attatgttat aatgatgttg
    23341 tgatgacggt tttagaattt tgacaagcag gagcgtgaag taatgctcct tttttctttt
    23401 ttgaaatata tcttattctt taggcactaa ttgcaacttg taatcatcag aactatataa
    23461 tttataacct cttaccttca agtctaattc tagttgactt aaacttttat aagcaccaaa
    23521 tgaaccatat ccattctgca tatttataat aacataccca cctattgttt cctttaaaga
    23581 aagaatcata tataacttat taccaccatt aatttcaaca atatctccta cttttaattc
    23641 tctattattt tcagttactt ctatcaacat attatattat ctccttactt taattaatat
    23701 tttttagaaa ctctcccatt ttcatttcat caatgttttc attaattcct ttcatagcac
    23761 ttaagttaac caaaggcacg cacaaatccc atagcttatt tacatctaaa aattgtattt
    23821 ttgaattaaa ttctttttca tcaacgcatt ttaatttatt attcatatta attaattcat
    23881 taatcattga atttttaatt aaattaagta taccatctcc tatttctttg agatcccatc
    23941 ctcttccatt taatttctta ttatgtatct ctataccttc tgcaatataa tcttctaatt
    24001 ctaaaatttg aaacattgtt aaaatagttt ggcaaacatc taatccttct gcaattatat
    24061 ctttggtcat tcctgtatct atagcctctt taaactcagt tacttcttca tcaatttttg
    24121 ccaactgatc ctctaccttc caattaggta tactgctaat taatttcaaa tccatcttta
    24181 atatctccct tcattcattt attgaagtcc caaccttttt tgagtatgct tattcatgaa
    24241 tattatttca tatctggcct tcttcacttt caagccactt ttttcgaccc tctaaacatc
    24301 ctcctaagca atcttgaaaa tcataagcac aactagtaca tccacttccg ccatttgcaa
    24361 gaaattcagc catttctatt ttgctcatat tttttatctt ttcataatta gtcatcttct
    24421 tattcaagtt acttccaaat aatctgtcat aacatttgtc acatacatct tttccgacac
    24481 tcattatatt accttcatca tcaattctga atacctttat gtttagaatt tctgctattg
    24541 tttcaccaca aaaatcacac tttataacac aactcatctt taataccccc ttttactttc
    24601 tttagaactc atatcttgat acatacatcc acaatcctta cattgccaca ctattccaat
    24661 taatgtttca atcttaaata tattaccatt acaaattgga cactcatttt ccctaccacc
    24721 aattatttta gttttcattt tacctccaat aaacttaaat catttattat ttacctagta
    24781 tattaatttc agtttttaat tttttaagtc tcctatatat tgacgtttgg ctagttccaa
    24841 gcactttact tatttcttgt tgagtataac ctttttctct tagacaaact attttctgta
    24901 tatcttttat ctcacttctt tttatacaag atctaataac acgaacattt tctatatcta
    24961 cattttcatt gccagccaat ctatcttcta ttcttatctc ttctccataa ttatctctat
    25021 aaatacactt ttcaaaacta tctatatcat ttctataatg tttcttaaca tatccagtcg
    25081 taaatctttt caaatgtcct gatatatacg aaaatgctat tgtagaaaat tttcctttac
    25141 tttcatcaaa gcaacatgtt gttttatata atcctatata tccttcttga aataaatctt
    25201 cttttaaata tggatacctt cttaaaaagg atttgaaata tctatttata ataaaatgta
    25261 caagatttat attatcttca aataatttat ttttctcttc taaacttctc atataatcac
    25321 catccaattt aaaatggaat atcttcatca tctaaaactt gaaagcctat ttcttcatcc
    25381 tttggcttgt gatcaagcgc attaacaatt tttgttctta cttttgtgaa agtttttttc
    25441 tctccatctt tttcatattt ttctatcctt atttggcctt gaattgaaac cattcttcct
    25501 tttgcgagat aattagcaca aaattctgct gatcctccca taacttcaat tggtataaaa
    25561 tcaacttctc tagttccatc cttttttgca taacccctat caactgccaa tgcaaaagta
    25621 gcaactggag taccatactc tggtatatat cttaattctg gatctcttgc taatcttcca
    25681 actaaaacta cattattcat attgcaatat ctccttcatt gtatttattt tctctatatt
    25741 ccatttgttt tacaatctca actgcttcat taaaggcaca acctctttca atttctttat
    25801 ttgcagacat aattgcatta gcaattttgt ctgtaaattc tgctctttct aaaatgttca
    25861 tatgagaaat acttttcatt taactccctc cttgttttac taagaggact tataataagt
    25921 cctcttagat tttcaattaa gcaattatat ttacattttt aaaatctttt aattcttctt
    25981 gcaagtacgc tttaatattt aacattgctt gatttttcca tgctccacca tctgcttcat
    26041 ataatcctgc tgctggtcca gtactcattc tgaaaataaa ttcactttct ggttgctcta
    26101 cttcttggaa agtacgataa ggaattaatt ttactctgtt tggaactatt gcttctgata
    26161 cactagcgac acctgttttt attgttacag attgagaaac cccatcatct ccaacatttt
    26221 ttactgtaga ttcttggata tttcctgcaa tttttaaaag tgtatctcta tcattatttt
    26281 ttataaatgc tgattgtagc attatattaa aagtgtctaa atctaaaggt ctgtcaaatc
    26341 ttttatctgg aagtagtgct acacattcaa tataagtttc tctatctcta ttttttctca
    26401 aagcagattt taaaagcact tttcttggag ttaagacttg tattaataac aactcatctt
    26461 cttcatgatc tatgccactt tttatataat ctactaaacc actcaatgta gttatttcta
    26521 attcattagc ttttggttct tctattattt ctaatgcatc tgttgtgtac ttaaacccat
    26581 ctacacatac tgtttctctt tttcctaact taactaaata ttctattgca tttcttatca
    26641 tttttttatt ctcctttttt atttaaattt atattttact tactttctta aactactttg
    26701 aacttgcatt tctgaaatca attacactag cactattttc ttctgtttca ttagtttctt
    26761 cactatctgg aatatccatg accaactgcc ctgctacttg atttttaaat tcagacgctt
    26821 caactcttcc tgtctctaaa tcttttccta tcagaacttt agttgttgta gcctttgctt
    26881 ctactatagt tgctttagat tttatatcta cttcagcaag atctctatct tcatttgctt
    26941 taaacactat ctctaaacta atttttcttg tttttttaaa atctgtattt ggatctgcaa
    27001 tattatctaa aactttttgt aactctgtat taaacttttc ttttagaact ccacctgcaa
    27061 atttttctaa tgctatcatg taaaatcact cctttattta tttaaaataa tatttttcta
    27121 cgaggaaatt aattaataaa ctttgactat aatttaataa catttttatc aaaatttttg
    27181 attttatttt tttctctcta tcattaccat cttttaatag tttttgacaa attctttaaa
    27241 cctgtgtttt ttgtgtaatg taatcgtatt ctgcaatcat tgcaattact atatttatta
    27301 atttttttca atattgattt tcaatacctt atgaaatttt aatttcaata ttgaaaaacg
    27361 gtattttttc aatattgatt ttttaaatta ttcaatattg atttttattg taaaatcatt
    27421 acttaataaa atttcctatt ttttatcctt ttttaaaata catcttctgg aatttctgct
    27481 tgcacatatt gtttatttac atcatatttg taatctctat ttaactcttc actagttgtg
    27541 taaagtcttt ttcttctttc tgaaaaattc attttgaaac ttatattctg ttttcctgtt
    27601 attctatttt ttaaaattaa taaataagtg tcgtctgcct cttcttcttc atcaataact
    27661 ctgtaagttg ataaaatata atctgcataa ttaacaatct cacttgctcc agacacatca
    27721 tacatagttg gtttcatgtc acccatattt tttctaggat gtgcaactaa aactattact
    27781 aagttatatt ttttagcgag attttttaag ttgcttacta tctgctcttg tctctcatat
    27841 ttatctgcct tattgccagt ttttattgtc attagattgt caagaacaaa gagcctaacc
    27901 ccttttttta aatataaatg ttctatggta tttagtagat ttacttcatc acttattgca
    27961 tcttcatcat agagaaaaaa tttatcttct atccagtctg atataagctc aacggcgtaa
    28021 tctggaatat ctgtgtatgt tcctccatag acacttttat attcttttat gtgataatca
    28081 ttagctacag tttttctaaa ccaatccata gccatgaaag agggtaattc tccagaatac
    28141 aaaaatgctt tttctttatt tgcaattgct tctgctataa actggttaac tatagttgat
    28201 ttaccagatg caggtttacc agtcaaaatt gtaagagttc catatctaaa tccatctagc
    28261 atattgttta gtttattaaa acccgtgtct atggacttgt gtacgccttt ttcacgtttt
    28321 atttttgatg cgtcaatata attaccattt atactactgc tcgtcgtcac aagtgctttt
    28381 aaatcgttta tatctacaat caattcatct atacttgtat ctgaatttga attattaatt
    28441 aattcatttg ctagctctga aatttgtctt ttaagtaact tctcttttat gatttttata
    28501 tagtgatcta tgtttgaagt agttatgact atagttgtta aactggtaat gtaactaact
    28561 cctccaacgt ctactaatct atctattttt tctaattcat tttttaaaag caaaagatct
    28621 agtggtttat ttgagtcaat tattttcttc atgcaagata aaataatttt atgtgactca
    28681 aagtagaaat cattttcttt taattccctt attttgtaac ctatagagtc atttataaga
    28741 aaacttccta gaatgttttg ctctgcttct aaagagtaat tcatttaatc acccccagct
    28801 tattattaaa atgtcacaac taaaggaatc accctttcga cgtcatacaa taccccacaa
    28861 atacgatctc ttggaacatt tttgtaaact aaaatatcaa ttatgtcggc aataagtttt
    28921 gcatagtatt ttggagtttc aaatccataa cttctataag caagtttttc ttcaacaaat
    28981 tgttcgcatt tacttcttgt acttgttcta gtttcacaac ctttactttc aaatatcatt
    29041 tctcttattt ttggatcagt tcttaatcct tcaataagtc tttgtatatc ttgatctgta
    29101 agttcatgtt tttttctttc cacttagcat acctcctgtc tttaactatt tagttgcttc
    29161 ttttgatttg aaactattct acaccatgat aatttacatg ctataattaa tttaaacata
    29221 cttccaatat gtttaaatta attagaaagg tggtgagagt atgagccaat atcgagttat
    29281 aagcaaagaa tgtcctgtaa taaacgaaac catttctctt acttgcgaat attcagctcc
    29341 tccaggtact tttggatcta agttaatgtc aactagatgt gatggaagtc atatttgcga
    29401 ctcttcttct aatgaagaat gtcctattct aaaaaaactt cttagatagt agctttggga
    29461 ttttcatctg gaaaacaata cttaaatgca gtcactttta cacctgttat tttgcttaat
    29521 ttagtaaaag aatgatacat acaggtaaaa tctccctcac attcttgcag gtaatcgcaa
    29581 acttcacatg atgtgggggc ttctctattc cctctaaaca ccatctccat aacttcattt
    29641 tgcaaatgtc ttgcaataca atgcaattct ttttcagtaa gttctaactt ttcctctttc
    29701 atgaacaacc ccctaattta tttaacattt aattctttat ctgtttcatt gattttataa
    29761 ctaaatttaa ttgacctatg ctttaattct acaccatgat aatttgcatg ttataattaa
    29821 ttggagcata ttgacagtat gttcaaatca attatagggt ggtgatgaat atgtgcaaaa
    29881 ttaaaacctt aagagaaaaa tgctctctta ttgacgataa agttgaattt tctggtgaat
    29941 ataaaacttt tcctggattc actggatcaa aacttttaga atgcgaatgt aataaaaatt
    30001 acatttgcaa aattcaaata atgactgcta atagcaactg tcctcttttt aaaaaagcac
    30061 gtcaattaca atactaaaat tttaatttac taaatttatt tagtaaaaat atctacacct
    30121 gtcatttcac ttaattttat aaaagtgtct atatgggtga aatagtcctt gcattcttgc
    30181 aagtaatcgc aaacttcgca tgatgtaggg gcttctatat tccctctaaa caccatctcc
    30241 ataacttcat tttgtaaatg tcttgcaata cagtgtaaat ctttttcagt aagttctaac
    30301 ttttcatctt tcataggcaa cctcctaatt tatacttaac taaatatctg acttaatatc
    30361 gaactcaata ttaaattggg caagcttatt aatcgattct tggagttgac aaattcgctc
    30421 attgacattt tctatcaatt cctcaaattc cttaatatcc ctaacaccta agaataactt
    30481 cacattagaa ctattcaaat aaacagcctc ttctttttca ttcatcctat ctacctccta
    30541 tactatttag tttttaaagt tctttaaaac cactgagtgg attcttagcc tttgtaactg
    30601 gttgcaatat acctttgcta gtatcagcat tcccccaagt caacaaagct ctgtaatgac
    30661 tcttatatct actcccttta ccatttacga tatagttttc taaactaaca attttatcat
    30721 gtatgtattt cttcccaaac ttgcttataa gtttgtcata ttcatcttta gttagtttta
    30781 ctttttctat atctaaatct aaaaatgata ggtctaaata atcagtttta ggttcatttt
    30841 gtggagtggt attacttttt atataagtct ttttattata agtcttttta ctgtgcacct
    30901 catgcacaac aggtagtgca tgaggtgcac tagggttgtg catcatattc actacctgtt
    30961 gtgcatcata ttcaccacct aatttagctg ttaaaacaaa atatatgttg ctttcttttt
    31021 catttgagtt actttttatt ctattttctt ttttaattaa ccctttattt tctaaaattt
    31081 taactacgct tttggcttgc ctttcactac atccgcattt caaagcgatc gttttatagc
    31141 ttggaaagca acaactttca ttatcagaat atcttgcaag aactatataa atcatttttt
    31201 catagattcc taaatcttct ctatctacta gatcattctc tagccaaaac cattcccttt
    31261 ttctagcatc tttcaagaga tccatttcat cacctccttt ctaaaagata tttcttagtg
    31321 taaaggtttt agattataat cattatattt tatatcatct attgtcaata ttttagttgt
    31381 aatgcattct aattcattgt catagtactc caatactatt tcgtttttat ctatatgagt
    31441 aataacgcct tttacttcta aatttaatct tatagaggtc attttatatg ttttatttaa
    31501 gctaaacatt tttgtaatca tttcatcatt cactttaata cctcccaact tatgaatgtt
    31561 ttatatccat aaaattattt tctgacaatt catttgctat cttcatacct tccataattc
    31621 caagaactcg atctctctgc ttgtctttta atgaagaata caatattcca aattctgttg
    31681 attcttttat gctctttgat attttctttg tatctacctt tttcattatt attcacctct
    31741 cttgtaatat gattttatta taaatcattc atgtgaaagt ttcaataaaa atatgaaaat
    31801 ttcacaatta ttttttattt tttcattttt gttttgattt tttcacattt atattgtata
    31861 attatactta agaggtgatt aaaattggaa aatataaagg atgaaagtgt tttaaaaaca
    31921 ttaagagagg cactggggtt aaatcaaagc gaattagcaa atattttaaa aatgacacct
    31981 tctgctatat ctaaaataga aaaaaatggc aagcctttaa gtgatagaat ttcgtctcaa
    32041 ttgagcaaag aatttggcgt tagagaagaa tggcttaaga acaaagaagg caagatgttt
    32101 gcagattcat cagatgatga tttagttgca gaaatagcag caaatataat taattcagat
    32161 gacaagttca tgaaaaatgt cattatagct tttaacaagc ttacagaaga tcagagaaac
    32221 tttttaatta attttatgaa agagttagat aaataaagcc gttaaaataa cggctttatt
    32281 tattgagaat tgtttttata aatattaaga ttgattttaa tttttttaca tcatttgtat
    32341 tttcaattga atttataatt tcctttttta aatctcccat agaatccccc atatttttaa
    32401 aatattctga tttatatttt tacaattaca ttgcttctga aaaataacag acttccccct
    32461 tttacaattt ttaaattttt atcagctact aaattaatgt tttttgcttt tgtatgtcat
    32521 gccttagatg agtttgaaaa tttgattgta ttcaatattg aaaattaaga tttttgatta
    32581 tttttaaaaa tattttacgt acgtagtttt atatgcattt tatatgtaat ttcgatatat
    32641 atcttcttta tgttacaatt gtaacacctc atttaacatt aatcaaatgt aaaattttac
    32701 caatttaaat tattttttaa aatttaattt tacattaata gaatttattt atataataaa
    32761 ttaaaaaagg actatggact atagaatttt acttctatag tccatagtct tcctataatt
    32821 taggattgat ataaatcttt tagaaaccag ttatttctga taactcataa gatgcaattt
    32881 ctccatctgt attcattccc attgctttga acttccaagt accatctttt ttaagatcgt
    32941 ttatattatc cattgcagta cctagttgat ttccatcctt atcatataaa gtaaatgtta
    33001 cttgtgcata tgatatatca tgaccactgt tgtttttaat aacacctttt atatacgttg
    33061 ccatactatc agtttctgat gttatatctc caactatttc atatttttcc tttttaggct
    33121 cttctggttt atctacacta gctgttttac ttgttccatc atcattactt tcagcagtat
    33181 catcagtacc acttccacta gtaccaatag cgtataataa aactaaaact actacaacta
    33241 taaaccaacc tcttttataa aatggttttt tgtttttagc cccacagtta ggacatgatt
    33301 tagcatttga tgctatttcc tttgaacatg atttacattg aatcattttt gacattttaa
    33361 aatccccctc taattaattt atgatgacct caatcatatt atagtataca actaatgtct
    33421 aaaactgtaa tatcatgaca tcaaaagaat tttattaggt atatagcaaa tattttgttg
    33481 tactaaatgg cattcaataa agactttcac ttatattgta tacatttttt cgaactgctt
    33541 ttaagttata atattaaata aataatttat ttaatattat aatacgtctt tataattagc
    33601 atattaaagt attttttata atcagaatta attggaaaaa attggaatgc ataattagac
    33661 atttttcatt gtaaattaaa ttctattgta atagtattat agtatatcaa aaaacggggg
    33721 tgtagtactt gaaaataaaa aagattccag agtcagaact tttggtaatg atgtacattt
    33781 gggatagtga agatgaagaa gttgcatcta ctgaaatttt gaaaacatta ggcgaaaaat
    33841 atgaatggaa aaaatcaact atgctaactt ttttaaggag attagttggt agaggatttt
    33901 tagaggttgt taagaaagat agatttacat actacaaagc attggtagaa aaagaagatt
    33961 atttgaaagt tgagactaaa agtttcttta gtttctttca taaaaattca tttgaaagtt
    34021 tattaagtgc tttgcatgat gatgaagaag tatctgaaaa taaattaaaa agtttagaaa
    34081 agtttatcga ggattgggaa gaagataaat agagagccaa tataatttta tttggttctc
    34141 tatctatata tttgaccttc tatttccatt tttaagcaaa tttgtatctt tttaggtaaa
    34201 taacatcaag tttgtaatta aaacacgtca gacaagcctt taaatgcttt taagctatat
    34261 ttaataaact ttatttttat caatttcctt tttccactca tcaaaattac aaatccgata
    34321 aacttttctt atttttggaa cttctctaag tttttctttg tcatattcat atacatttac
    34381 tttttttgtt tcaatgtatt tattttcttc taaagtttta acagaagaag ttatgatatc
    34441 taaattattt ttacttttag aacttagtcc tatattttct gctataaaac tattagttgc
    34501 aggtttaaaa ttagtttctg tagtgattgt tttaagtaaa aaatatactt ttattgtatt
    34561 tgatttaaat ttattgacca tttttttaag catttcataa tttatcaatg caaatttgtt
    34621 taaatcaata ttattttctg acggtaaaca atatacaatt ccatttaggg tattttctat
    34681 ttttagaatt ttaaaatcta acttctctag tttttttata tttctttgga ttgttgcttt
    34741 atttacattc agttcatttg ctatttcttg aatatcaagt ttattcttat acaaaattct
    34801 atttttatgg gtattaaaaa tgtcactatt ttcagcactc tttattaata gtgctccata
    34861 ttcttttata taatattgct tatcttttaa acaatatgtt gctattggta agtaaatttc
    34921 tttctgaatt tgttttctgt tttcaagcat aaaaaattct tcatctccaa tcttttataa
    34981 gaaattattt ttagtaagtg tttaaagtat actagaaaaa taaatactaa agttttttaa
    35041 tataaaaaaa gaatagttca gaacatcttt tctagtcttt aatagttttt aaaacagttt
    35101 ttaaagtctt taatagtttt tagacaagct ataataattg gtatatctag gtttcagaga
    35161 tgaaaggtca tcattttaag ggtcaaaggt catcatttta agggtcaaag gtcatcattt
    35221 taagggtcga aaggtcatca ttttaagggt caaaggtcat cattttaagg gtcaaaggtc
    35281 atcattttaa gggtcaaagg tgagcgtctt tcaaaaatag ttgacctttt tataaattag
    35341 gtattataat ttatatgagg tgatagattt tggaaaaaaa agaaatttta atgcaaccaa
    35401 ataatttaat aaaaagtaaa tatgatttta caaatgtgga aaataaatta ttttacaaaa
    35461 tactttttaa tgctcagaaa cagcaaaatt catcttatgt tacaactata tcaaaagaag
    35521 aactaaaagt atttatgaaa aacaataatg attatgagca taaaaatata aaagaaattt
    35581 tgaacatgtt tcaacaaagt gttttggaat ttgactatat agaagagaca actggcaagt
    35641 taaaaacttt tggaagtggt ttaattaata catatgaatt ggatcataca gaccaaattt
    35701 atacaattat gatgcatgaa gttctataca atcatataac tgattttgta aaaatgcaga
    35761 aaaagaaaaa tggatataca gctattaatt tatcagtgtt atttaatttt agaggtgcat
    35821 atactcagag gttatacact ttatttagac tatggagcag agaaaataag gaagtggaga
    35881 taaaatataa gttagatgaa ttaagatttt atttgaaatt aaaagataat gtatatccag
    35941 agtataaata ttttaaacaa aatgttctaa aaagagcaat gaatgaaata aataaaaaag
    36001 gcaacatggt tgtttcaata aaagaagaaa aaagaaaaaa tagaaaaata gatgaaataa
    36061 ttttttctgt gatagattat gaacctagaa aatattttga taaagatata ttggttgaag
    36121 attatatacc taaaaagaag caggacaatg aagtgtcttt tagtttttat ataccaaatg
    36181 aaaatatttt tacaacaggg acgattaaac tatttaaaga agattttaaa gagtttgatt
    36241 ttaagaaaga tgtttatctg aatgctttta ataagtctat tgctatagca tttgaaagag
    36301 ataatactga tattataaat acaaagaatt atgatttttt taaatcaaca ctttcaaata
    36361 aaataagtga agttataatt catcaaaaag atgatttaaa gtttaaagaa gaattagata
    36421 aatattggga tgatgaagat gttaaaaaag aagaaagaga agttgattat agcaaagatc
    36481 ctgtcagact tgaaactgtt aggagaaatt tatttgatca aggtctagta aatgaagaag
    36541 gatattacta taaataaaaa ttggagaaaa taaaaaatgt attatgttta taaatatatt
    36601 gatccagcta cagaagaatg tttatacgtt ggaaaaacag aagatatata tgcaaggcat
    36661 tcatctcatt tgagtaataa agaagaagat tggtgtacta aagaattaat attaaaatat
    36721 attgaacttc cacgtagata taatatggat ttttttgaag tttatttaat aaatgaacta
    36781 aatccaatat ttaataaact aagtaaaggt gatatggata ctgaacacat cttttttgat
    36841 tacaaggaag aagattggag tacatattca gaagaagatt ttaaggaaaa gacaaaggga
    36901 aaaagaagat tcagagaaag aaagatagat atgaaatatg aagtaagtaa aaaatctatt
    36961 aattttttaa agagaattga aaagttagat gccaaggttg aaatgttata tgaaaataat
    37021 gttttaagtg tgctatataa ctttgaaaat atggatatag aattttgtga gaatgaatta
    37081 gatattaatt tgatatcata tgattttgaa acattagaaa gatttggaag ttgttctttg
    37141 atttcagttt atcccagatg gaaagaatat acagcaacaa ataaaaaaat acttataaat
    37201 ataacattag agattaggat tggatctttg gctaatttaa tggaattaat gccaacattt
    37261 gaatataaaa ttactgagat tttccaacaa atagaggata tttttcacat actagaagtt
    37321 ggatatacat ggcaagaatt atttgaaata tataagggct gtaaatatga caattaagaa
    37381 ttggagaaaa taaaaaatgt attatgttta taaatatatt aatcaagata cggaagaatg
    37441 tttatacgtt gggaaaacag agaatatctg tgatagacac gctagtcatt taagtaataa
    37501 aaaagaaaat tggtgtaata agaatttaag acttgagtat atggaattag ataataaata
    37561 tactatggat ttttatgaga tttacttaat aaataaacta gagcctaaat ttaatattac
    37621 tggaaaagga gaaatggata tcgctaaaac atcattttcc tataatggtc agtgggtaat
    37681 ttatttagaa agagacttaa tgtcgagttt ggcatcaaag agatatggaa taaattatga
    37741 agtgaatgct aagatgttag gcataatgag aaaattaaag aagataagca gcaatgaaga
    37801 aatatttatt ggaaatgaaa atataagaat aaaatattac tttgaaacag tactagaagg
    37861 aattaattta gaaccatgca tattgagtgt agcatataag acactaaaag aaagtacagt
    37921 tggaacagta atctcagtaa aacgtgaata tttatcagaa aatgtatgtt taatcattga
    37981 tagtattttt ctaaatgaaa taatgaaaga tcctttaatg tctaaatcag atattaaaga
    38041 tttaaatagc caagtcaatg atattcttaa aataataggg gttgattgtg agtggaacaa
    38101 gctttctgat tattgtagtg caaactctta actgatatta caaaatatgg tataattaaa
    38161 tttagcaaga agagcgtaat atacaatcta tagagtggag ttcatgtaca aaagattatc
    38221 ctcccaacga ttcgagggga ggtgagttac atggataact ttttgtttaa tgttttagct
    38281 agtttaacag ctagtgtggt agtttactta atcagtgaac tattcaaaaa agcaaaaagc
    38341 cactctcgtg caaagagtga cttacaggtt gaatttaaat ttatatttaa attcaaaaaa
    38401 taaactacgt ttattatgaa ctccactcta cgcctaaata gattgtagtt cttcttgctt
    38461 ttattatatc acaaaatctt taaaatgata caattttata tcatttctta ttattaagaa
    38521 atagaaaaac actttaattg attaaagaat aattttatat aatcttgtaa aaatataaaa
    38581 agaacactta atatattagc ttgaaaaata ttaagtgttc tttttttcta ttctatttaa
    38641 ataaaataga atagaaaaaa tgattgaaaa tattattcta tcgtgataga atgaaaaaaa
    38701 cagaatattc taccgtgata gaatattctg ttttgaagaa gaagtaaata ttatttgaag
    38761 gttaaaaata taagaacaat ggagggaatt atggaagtaa acctacatac aggaaaagat
    38821 gaatttttta agaaccatga agtagcaaaa aactatatag gtggattata taaacttatt
    38881 gatgtttaca aattctcaat ggaatcagtt tccgttttaa ctagaataga tttagataag
    38941 ttaaataatt tctatgctgg agaagctagt ttaaactatg atgaattgtg cttaatagaa
    39001 agtgtaatta taactccttt ggtaggagca tttgaaactg ctgagcataa ttacaatatg
    39061 attattaagg cacgtaaaga aaggaaactg taattgagaa taatataaaa aaataggggg
    39121 aaaacaaatg aaaaaaacaa ttgctttatt actaacgctt tgcgcagtaa tgattccatt
    39181 tacagctttt gctgatggaa atgattatga aggtactgga aactcacaaa cagttgaatt
    39241 aattggacaa ccaaactcag attcgccttc aactaaatct attgtcgttc caggtggtac
    39301 tgctaattta acagcaatga gtggagggca gttatcttgg agagtaacat ctacgcttgg
    39361 aaatatttta ggatttgaag gagaaataaa tgtatataag gctggcaaaa ttgtatcatc
    39421 tcaacttgta ggctttagat tagctggaac tactaaaact ggtgtagtaa gtttcaaagg
    39481 gctaaaaaag ggttcatata ttgctaaatt taaaggaaaa ggtgtgtgta caggaggtat
    39541 tatattattc tctagtccag ctgaagcttc ttttacaatt agataattat aaaaagtaga
    39601 aaagagtgtt tagaaatata tgaaacactc ttttttttat gcaaaattat aaaaagtgtg
    39661 tttttaatta tatacaaaat gataaatttt ataaaaaatt ataaaatatt ataaaaaata
    39721 tcttataaat ctattgaatt actatgtgaa atttggtaaa atatatgtaa atattaataa
    39781 attttataat attttacaaa aaaggaagtg gttgaatgag taggatagtt ctaacattta
    39841 aaaacaatga taaagaaaaa gcaattgaaa agtttttaga tgagaaatta tcagctacag
    39901 cttatttaaa agaactcgtt tgggaaaaaa tgaatgaaaa aaaggataat gctgtagttg
    39961 aacagaaaaa agaaatagaa gatccagcta acaattttga ttttggaagt ttagaataaa
    40021 agggggaaat aacataaatg agtaaattag gtatagatat tggaaattat gcagttaaaa
    40081 caagtacaga tgatattttt gaaagtaaag ttacagaggt aaaaaacttt ggttcagatt
    40141 cagatagcat taaaataggt aataaaacat attatttagg cgaaggtgac gaggaaataa
    40201 atatagttaa atatgagaaa gaaaacttct taccgctttt acttggggct atatgcagaa
    40261 atacagatga tgaagttatt gatctagcat taggtctacc agtgaagcaa tttggaggat
    40321 taagaaaaga tttgattgaa aaattacaag gaaaagaata tcatgttgaa tttgaaaaag
    40381 gaaatgaaac tactaaaaga gatataacaa taagatctgt tcaagtgttt ccagagggag
    40441 ttacaggata cttatattat gcaaaagaca tagttgacca aattgcagga agagatgttg
    40501 ttttagttga tattggtgga aagacaacag atattgcgct tgtacaaggg aataaagcga
    40561 ctgatccata ttctgtaaat gttggaacga taaatatata tgatgcaata aaaaaatctc
    40621 ttgaaatgga tgaaagattt ctgggtaaag ttgagataaa aagagaaaaa atacaagact
    40681 acatagacaa agggttttac ctaaatggtg aaaaacaaga tataaagaaa aatatagatg
    40741 cgtctgttgg attatttaaa caaatatata atgaattgaa attaaattac ccaatatcaa
    40801 cttctgctgt tgtcgttatg ggtggaggtg ctaaattgtt aggtgaagca tttaaaaaga
    40861 atatacctgg cataatagtc atgagtgatg tagataaaca tgtttttgca aatgcaaaag
    40921 gatacaaaaa aatgatgaaa taaaaaagga tttagtttat agtatgtata atagattaca
    40981 aatatatgat atttgtaatc tattattttt tgaggaggtt ttatatgact tggagtgatt
    41041 tgaaagaaaa aaatattaat gaagttgttg attgtattaa taataaatta agtgaatttg
    41101 aaagtcttaa aaaagttgga gatgaattag gggcaaatga aagtactata aggaagtggc
    41161 ttaataagaa aggatataaa agagttggaa acaggttttt agaagataat aaaagttatg
    41221 atgacaaata tcagatagat agcattaaca gtaagattga tgatgatatc aaatatttat
    41281 ctaatgaaat tgataatata aaaagtgtga tagaatggtt taaaactaaa gatgataaat
    41341 gtcatataga tagcattaaa gaaaatgata tatctataga tcttccagat cagtctataa
    41401 aaagaactac aattagggta aataatacag tgtgggaaat gtttaataaa tttgcagatg
    41461 agaataaaca ttatgataaa catgatttgc taagtcaatt attgcttgaa agtctattaa
    41521 aatatgtgaa gagcagatga agtgagatat ttaatcttct tcatctagat ttttaaattt
    41581 atgcacagtg gacattaatc gtataaaata tccatctaaa taatctggct ctaaatcatt
    41641 tattttatat ttgaaatatt ctttttctgt aactataggt atgtaatata tgtggaaaat
    41701 tttaattctc ttaagtagaa ctcttttttt aacaagtctt ttaagtagaa tttttgtcgt
    41761 agatttctgc cacttgtgtt tttcattgca aatgcgaata atttctttcc ttgatattct
    41821 tgaatttctt ttccaaaaga ttttcattac aattaattcg ccacgtttta agctttgtat
    41881 taacatgatg cactccttgt aaatgtttat aatatagtag cattttttta tttaagatag
    41941 tacaggtaag ttattccaga aaaaaagcaa cgctagaatg tttcaaaact aaatatgata
    42001 aatatcatat aaatgtcatt aaaaaatgat ttatttttat tgtgataagt taaataaaaa
    42061 gaattagaag ataataaaat tttctagtct ttttatttat ttaatcttag atttaacaga
    42121 tgcgtaattg caacgtataa gattattaaa agaaatagaa aaataaagaa agcaaaagat
    42181 gatagttttt ttatctatta tcctttgctt tctctaatat taggatttta ctatgttttt
    42241 gtcaattgca ttgtttctgt atgctctttc aattctattt ttaccttgaa gaaaataatc
    42301 gctatctaat tctattccaa caaactttct atttatattt gcacaagcta ttcctacact
    42361 gccagaacca aaacaattat ccaaaacaag ttcattttcg tttgtataag tttttataag
    42421 atattctaaa agatctactg gcttttgagt tggatggatg caatttgttt ctttattaaa
    42481 atacaatgta ttattaggat aatttgtata ttcttgaaca tattcttttg ataaagtatg
    42541 atgtctatat attccatctt tatttttatt tttcttttta catgtaatag gtttatctaa
    42601 tttttctaag ttttgaggat tgtataaagg agctttttta tagaatacat taatatcttc
    42661 tacttttcga agtggctggt atttagaaaa tgcgaatcct gtagatttat tttaatccag
    42721 taccaactgt atttatagtt tttaatattt gaatttatca aactagtagt aaatggttgt
    42781 gctgaaaata gtactatagc tccaccatct tttataattc tattatattg attccagatt
    42841 gatttgaaag gtattattgt gtcccattta caattagttg tgccataagg aagatcacat
    42901 agaattaaat ctattgattt atcttctatt agatccatta tttctaaaca gtttccattg
    42961 tatagtttat atttttctat gttaagcatg ttgcaagtcc tcctaagttg aataaatttt
    43021 atattgattg gatattacaa caagttatta tttgataaaa tctaatgctt tgtaaagtgt
    43081 atcaaatcta tcattacctt ttatcattgt gtaatgctct ttagtaatag aacctatctt
    43141 ctcacatgct ccaccgccta caacataaag attttgcgtc tgacctggta cgtaatcttt
    43201 tatatcacat atcagtattt tcccatcatt ataaccccaa ccaactacag ttgcagggat
    43261 tttgtcaact tctccatcat aaacaattgt atgtttgtac atctgtttaa ctccctcatt
    43321 atttatattt ttatttaata caccttctac aattaactta gcaatacctt catgacctag
    43381 tttcttagct ttatcataat cttctttatt atcacagaag aaactttcaa ttaatactgc
    43441 tgtaggcttt gaactattta agatatataa tcttttatct aatttagcac ctctattttt
    43501 aaatactgta cctagtttat cacatattct agttgcatac tctaagcctt tattactata
    43561 atataggact tctgaacctt
//
LOCUS       DAELJO010000010        42579 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELJO010000010 DAELJO010000000
VERSION     DAELJO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.010"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..234)
                     /locus_tag="KPB88_000505"
     CDS             complement(<1..234)
                     /locus_tag="KPB88_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG0052814.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQD"
     gene            451..1734
                     /gene="hflX"
                     /locus_tag="KPB88_000506"
     CDS             451..1734
                     /gene="hflX"
                     /locus_tag="KPB88_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0052815.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            1742..2419
                     /locus_tag="KPB88_000507"
     CDS             1742..2419
                     /locus_tag="KPB88_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG0052816.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2505..2948
                     /locus_tag="KPB88_000508"
     CDS             2505..2948
                     /locus_tag="KPB88_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0052817.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3253..3897
                     /locus_tag="KPB88_000509"
     CDS             3253..3897
                     /locus_tag="KPB88_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG0052818.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(3983..4360)
                     /locus_tag="KPB88_000510"
     CDS             complement(3983..4360)
                     /locus_tag="KPB88_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0052819.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4360..4746)
                     /locus_tag="KPB88_000511"
     CDS             complement(4360..4746)
                     /locus_tag="KPB88_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0052820.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            4925..5680
                     /gene="nadE"
                     /locus_tag="KPB88_000512"
     CDS             4925..5680
                     /gene="nadE"
                     /locus_tag="KPB88_000512"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG0052821.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5763..6482
                     /locus_tag="KPB88_000513"
     CDS             5763..6482
                     /locus_tag="KPB88_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0052822.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6649..7653)
                     /locus_tag="KPB88_000514"
     CDS             complement(6649..7653)
                     /locus_tag="KPB88_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0052823.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            7919..8815
                     /locus_tag="KPB88_000515"
     CDS             7919..8815
                     /locus_tag="KPB88_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG0052824.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRKTISALVETLPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            8970..10232
                     /locus_tag="KPB88_000516"
     CDS             8970..10232
                     /locus_tag="KPB88_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052825.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10308..11501
                     /locus_tag="KPB88_000517"
     CDS             10308..11501
                     /locus_tag="KPB88_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0052826.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12129..12923
                     /locus_tag="KPB88_000518"
     CDS             12129..12923
                     /locus_tag="KPB88_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0052827.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13018..14358
                     /locus_tag="KPB88_000519"
     CDS             13018..14358
                     /locus_tag="KPB88_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0052828.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNAKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14495..15727
                     /locus_tag="KPB88_000520"
     CDS             14495..15727
                     /locus_tag="KPB88_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0052829.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15767..16909
                     /locus_tag="KPB88_000521"
     CDS             15767..16909
                     /locus_tag="KPB88_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0052830.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            16920..17714
                     /locus_tag="KPB88_000522"
     CDS             16920..17714
                     /locus_tag="KPB88_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0052831.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17725..18747
                     /locus_tag="KPB88_000523"
     CDS             17725..18747
                     /locus_tag="KPB88_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0052832.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            18846..20222
                     /locus_tag="KPB88_000524"
     CDS             18846..20222
                     /locus_tag="KPB88_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0052833.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20554..20898)
                     /locus_tag="KPB88_000525"
     CDS             complement(20554..20898)
                     /locus_tag="KPB88_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052834.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21386..22396
                     /locus_tag="KPB88_000526"
     CDS             21386..22396
                     /locus_tag="KPB88_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0052835.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22532..23020
                     /locus_tag="KPB88_000527"
     CDS             22532..23020
                     /locus_tag="KPB88_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0052836.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23196..25124
                     /locus_tag="KPB88_000528"
                     /pseudo
     CDS             23196..25124
                     /locus_tag="KPB88_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            25301..25711
                     /locus_tag="KPB88_000529"
     CDS             25301..25711
                     /locus_tag="KPB88_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG0052837.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            25733..26035
                     /locus_tag="KPB88_000530"
     CDS             25733..26035
                     /locus_tag="KPB88_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG0052838.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26252..26944)
                     /locus_tag="KPB88_000531"
     CDS             complement(26252..26944)
                     /locus_tag="KPB88_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0052839.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(26970..28157)
                     /locus_tag="KPB88_000532"
     CDS             complement(26970..28157)
                     /locus_tag="KPB88_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0052840.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28493..30370
                     /locus_tag="KPB88_000533"
     CDS             28493..30370
                     /locus_tag="KPB88_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0052841.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            30682..32088
                     /locus_tag="KPB88_000534"
     CDS             30682..32088
                     /locus_tag="KPB88_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0052842.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            32116..32964
                     /locus_tag="KPB88_000535"
     CDS             32116..32964
                     /locus_tag="KPB88_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0052843.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            33625..34317
                     /locus_tag="KPB88_000536"
     CDS             33625..34317
                     /locus_tag="KPB88_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0052844.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            34314..35249
                     /locus_tag="KPB88_000537"
     CDS             34314..35249
                     /locus_tag="KPB88_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0052845.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            35397..36320
                     /locus_tag="KPB88_000538"
     CDS             35397..36320
                     /locus_tag="KPB88_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0052846.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            36304..37074
                     /locus_tag="KPB88_000539"
     CDS             36304..37074
                     /locus_tag="KPB88_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052847.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            37084..37866
                     /locus_tag="KPB88_000540"
     CDS             37084..37866
                     /locus_tag="KPB88_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052848.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            38243..38779
                     /locus_tag="KPB88_000541"
     CDS             38243..38779
                     /locus_tag="KPB88_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0052849.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            38811..39200
                     /locus_tag="KPB88_000542"
     CDS             38811..39200
                     /locus_tag="KPB88_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0052850.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            39237..39623
                     /locus_tag="KPB88_000543"
     CDS             39237..39623
                     /locus_tag="KPB88_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG0052851.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            39750..41192
                     /locus_tag="KPB88_000544"
     CDS             39750..41192
                     /locus_tag="KPB88_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0052852.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            41489..42304
                     /locus_tag="KPB88_000545"
     CDS             41489..42304
                     /locus_tag="KPB88_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0052853.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        15857 a         5005 c         7983 g        13734 t
ORIGIN      
        1 atcttgaaat ttactcataa tgtaattgta attatctttt ttatgaggta atatacaact
       61 actgcaatct ttgatacctt tatctgtata cttaaagttt ccaccacagt tttctcctaa
      121 agcatacaaa ggacaatagc aaaatagaca gttaaactct tctggtttat tagttttatg
      181 acaagggaaa aattcgcaat ctttgtgatt aaaaaactta taattttcgc ccatattttc
      241 ctcctaatta aataaaattt aaatttttta tactatataa gattttggtg ataatataat
      301 tatactaaat aattttatga atagtcgata atatagtatt attaatcata gtattaatgg
      361 tataattagt caacagaaaa ataatgtaaa aataatgtaa gtgtattttg taattagaat
      421 atgttttata acaaaaaatg aggtgattaa gtgaaggaaa ttcaagaaaa agcacttctt
      481 gtaggtctga atttgactac aatagtgaaa aaaaatgatg atatagacac taatgaatct
      541 atggaagaat taaaagaact aacaaaagct gctggggcag aagttgtagg tagcttaatt
      601 caaaataaac actctgtaga tgcagcttat tatataggaa aaggtaaagt agaagaaata
      661 agagcctata gtgactcttt agatgctact ttagtaattt ttaatgatga attatctggt
      721 gctcaaatca gaaatataga gaatgttgtt ggaagaaaag taatagatag aactacttta
      781 attttagata tatttgctca aagggcactt agcaaagaag gtaaattaca agttgaatta
      841 gcacaattaa aatatagatt gccacgactt tatggaatgg gtggtgagat gagtagaaca
      901 ggtgcaggga ttggtactag aggtcctgga gaacaaaaac tagaaataga taagagacat
      961 atacttaata aagcagcaga tataagacgt gaacttaaag aagtaaaaaa gaatagagag
     1021 acacagagag ttaagcgttt aaagtcaaat ataccgattg tagcacttgt tgggtataca
     1081 aatgcaggaa aatctaccct gttgaacgaa ttaataaaga cacacaagga ttatgaacaa
     1141 gaaaaagaag tttttgtaaa agatatgctt tttgcaactt tagatgtaac tttgagaaag
     1201 gctcttttgc caaataaaaa agaattttta gtagtagata ctgttggatt tgtaagtaaa
     1261 cttccccatg atttagtaga agcgtttaag gcaacattgg aagaagtaca gtatgcagat
     1321 ttaatcttac atgtaattga tgcaacaaat actagttatg aattgcaaaa aagtacgact
     1381 gaaggtgtat taaaagaact tggtgtaaat gataaaaaac atatacttgt atataataaa
     1441 gtggacaagc tagaattgga tatataccct aaaagtcaag aagatattgt gtatatttct
     1501 gcaaaacaag gcataaatat ggataagtta ttaaatatga tagaaattgc tttaatggaa
     1561 aatacttatt cagtaagttt gatgttacct tatgagagag gtgatatatt tagtagaata
     1621 aaagataaat ataatgtgga gaattttgag tatggggaaa gtggtataac gctagatgta
     1681 gatttagatg aagaagattt taatatctat agagagtaca tactagaaaa ataggttgaa
     1741 tatggatata aactgggaaa atatagataa tcttgaagat tattttataa cctatcttct
     1801 ttataaagaa tctaaaacag tatctcaaat tagtaaaatt agaaatattt caagtactga
     1861 agcaagtgag caacttatac aagcaaagtt aaaaataaag gaaatgcaaa aagatgattt
     1921 tgaggcatct aaggatattt tggataaatt tctagaacta gataaaatta agagacttga
     1981 ttttatggat agcttagatg acgaaaaaat ggtatacttt aagagaaagg ttttcaaaag
     2041 aatccttgta gaaaaaaatg ctgaagattt aattgtttta atttgggcga caggtgagct
     2101 aaaagatgat aggtttttga aattgcttca tcagctcaca aaccacagac actcagatat
     2161 tagaagaata acttattcag caatcagaaa gattgaatca ccaagtagta gagaagtact
     2221 gcaaaaaggt ctctacgaca aaaatgcaca gactaggcag tactgtgcaa aagctctttc
     2281 aaaattggga gatgaaaaca gtttaaagat tttacagcag ttgaaagaca agaataagaa
     2341 ttttgaaaaa gaatatgtgc ttagagctta tgacgaagcc attaagtcat tggaaaatgc
     2401 aaaaaatttg aaaaagtaat tttttattaa agcaatttgt ttacttaatg atgtatttct
     2461 aaagtttaac aaggtaataa ataaattaat gggaggggtt tcaaatgata gtaagacgtt
     2521 gtgcaggtgg agtagtgttt tatgctaata aagtacttat tgtaaaaaat gataggggtg
     2581 aatggacact tccaaaagga aagatactag gagggggact tccatatgag agtgctgttc
     2641 aaagagttaa agtagaaact ggcatagatg ctaaaatgat agatgtggct ggcgatacta
     2701 tgtatgaatt tttctcaaga agtagacaac aagaagtttg taatgcaata atgtggtatg
     2761 ttatggaagc ttgtaacact gattgtgtac ttgctcctga atttcaagaa ggtggatttt
     2821 ataaagtaaa ggatgcttta gaaatgttat cacatcataa agagcaagct ttagtagaag
     2881 tatcttataa aaagtttaag gaattaaaga aattagtttc agagggcgat gatgttccag
     2941 caaattaatt gtgaatttag aggattctga ggaagggata gccccttcct tttttaattg
     3001 aagcagtttt aagcgtaaat tatatagtct ctttaaacta tgatttatca atttagagat
     3061 ttagtagttt atatttaaaa tcagtaagat ctgtttaaaa taaaaattaa aaagtataca
     3121 attttattat ataattgtct tatgatgttg attaaagaat tattaaaagt ccatttattt
     3181 ttaaatagaa taattttata aatatatatt tgatgtgtat attagaaaat gattaaagta
     3241 ggaggaaata caatgagcaa ttacaggaca ttacatgaat ttggaacgga tgaaataacg
     3301 attgaaaagt ctgtttttat tggatatgct aagcctataa agagtgaaga agaagcatta
     3361 gagtttataa atgaaataaa gaaaaagcat aaagatgcaa ctcacaatgt gtgggcttat
     3421 actgtaggta aaaatatgaa tatacaaaga tatagtgatg atggagaacc acaaggaaca
     3481 gctgggatac ctacattaga ggttataaaa aaagaagatt tgagagatgt agcggttgtt
     3541 gttactaggt attttggagg aacaaaatta ggtgcaggtg gtctagttag agcttataca
     3601 aaaggtgcta aactgggact tgaagcaggt aagataattt ataaggtaat gtatcaagaa
     3661 gttaaggtta agatagatta tactcaactt ggaaaagtgc aaaatgagtt aatgaatttg
     3721 gggtatttta taaaagatac tgtatatgag gataatgttg aaatagtagt atattcaaga
     3781 ttagaagatg tagagaaatt atcagaaaaa atgattgata taacaagtgg aacaggaaaa
     3841 atagttttag gagaggaatt ttatttgtct gaacaaaatg gagaaatttt aatataatct
     3901 aaaaggttat tagaatagtt aataccttaa atagccaatt agtattttta agtgaaaagc
     3961 cattaaattg atttaatcta aatcaattta atggcttttt atcggatttt gcacagaatc
     4021 ctttttttac ttcaaataaa ccgactaaaa gtaaaaatat cccaaatatt ttttttaata
     4081 tatctgaaga cagataatta gctaatagtg aaccaaatac agctcctaaa accccaaaca
     4141 cagctatagg tataaccaat ttaaaaatta catttttatt ttttatatgt atagcaagtg
     4201 ctataagtgt catagggata gatgaaagta aatttacgct ttgtgctatt ttagtgtcta
     4261 tgccagcaag taaaacgagt gcaggtataa ggattgttcc tccacccatt cccattcctc
     4321 caataattcc tgcaaaaaat ccaattagag caaataacat taaaacacca tccttaatgc
     4381 tgcaattatc ataattattc caaaagaaat tctaagaaac tttccagtaa ccttatttag
     4441 taatttagct cctataatac caccaagaat acttccaact gcaactttaa ttgttaagtt
     4501 aacatcataa gtacctttag atacatataa aacagaactt gctgtcgtta agaatattat
     4561 tatagaaaga gcagttgcat gagatttatg ttcttctacc ttaacgatgt catttaatat
     4621 tggaactaaa agagtacctc ctccagaacc aaaaactcca tttataaatc ctgtaaaaac
     4681 tccaattatc gtatttttta agttaaaatc aaagtatttt ttctttaaat catcagtttt
     4741 tttcataaaa atcaccttct gaaatagttt taccaaaata ggcaaattat atttatataa
     4801 aataagatta aatataatct ttcatgtttg tttaattttg cataaaaaaa aacatattaa
     4861 aacaacatat taatatatgg tataatatat taaaacaaag tagcaaattt gagaggagat
     4921 aacaatgtca aatataaaaa tacaaataga caaaactgta gaatggctta taaataaggt
     4981 taatgaggca aatgcaaaag gattaattgt tggtgtatct ggaggaattg attcggcagt
     5041 agttgctaat ttaattaaaa aggcatttcc agaaaactct atgggagtta taatgagcat
     5101 aaaaagtaat cctcaagata gagaagatgc acttaaggtt atagagggat gtgatataga
     5161 ataccttgat ttagacctta tagaaccaca aagtgctata ttagacatgg tagtaggaaa
     5221 tttaaaagat aaacatttat atagagaaga atatttaaag atgacagatg caaacttaag
     5281 agcaagagta agaatgagta caatttatac aattgctaac aatctaggat atctggttgt
     5341 tggtacagac aatgctgcag aaatacacac aggatatttt actaaatttg gtgatggtgg
     5401 agttgatatt ttacctattg caaatttaac taaaggagag gtgtatgaat gggcaaaaga
     5461 gcttggtgtt catgaagatt taataaacaa agctccttca gcaggtcttt gggaaggtca
     5521 aacagatgaa gatgaaatgg gaactactta caatatgata gatgctgtgt tagaaggtag
     5581 attggatgaa gttcctaaaa gagaccaaga gataattgaa agacttcata gattaagtga
     5641 acacaagaga aaaacgcctg cacaacctcc taagttctaa tttactattt ataagatttg
     5701 tgataaaatt tacaagtgat gaaaattaga ataattaatt gtacgtatta aggagtggaa
     5761 atatgggacg tataggtaat ataattaata gaaaaggtaa acaagatgct caaagagcaa
     5821 agatatttac taaacatgct agagcaatag cagttgcagc taaggaaggt ggagcagacc
     5881 cagagtacaa tgcagcgtta aaaacagcta tagaaaaagc taaggcagat aatatgccaa
     5941 atgataatat agatagagct atcgctaaag gtgctggagc aggtgctgga gaagactatg
     6001 aaactatagt ttatgaagga tatggacctg gtggagttgc tgttatagta gaaactttaa
     6061 ctgacaacaa aaacagaact gctggtaacg taagatacta ttttgacaaa aacggaggaa
     6121 acttaggaac tagtggatgt gtgtctttca tgtttgataa aaaaggacaa atattagttg
     6181 gtttaggaga tggagtttca gaagaagaat tgatggatgt tgcattagaa gcaggagctg
     6241 aagatttcat aacagaagaa gatggttatg aaataataac tacaccagaa gatttttcta
     6301 gtgttcgtga tgagttaaaa gctaaaggat atgagtttat atctgctgat gtgaagatga
     6361 tacctcaaac tacaactgta ttgactgaag aatctcattt aaaaatgatg aataaattag
     6421 ttgatatgct tgaagaagat gatgatgttc aagatatata tcataactgg gaagtagaat
     6481 aaatagtata agcatattga aatttataag tattgaatca attttaacat gtgataaatt
     6541 ttatagaata agaaacacta aaatagacat acgcacctct aacctatatt tgcataataa
     6601 tatgtaagca taggtaaggg gtgtttttat atttataggt taagaaattt aaagtaaatt
     6661 ttgagctgga ctcgattgtc tataaataat tgatgggtat ataacttgtt gctgaatacc
     6721 atttatacgc ccagttactg tatcaataat cattctaaga ctacgttctg ctgtctcttc
     6781 catttttaaa tctataactg tcaaaggagg aatacttctt gccattagtc gtgaaggtcc
     6841 attacttgct gcaaatattt gaatatcctc tccaattaca attcccgatt catgaaatgc
     6901 attcatcata ccaagagcta ccacatcatg attacaaaaa attacttttt ttagtgaatt
     6961 ttttgcaagc attttttgag caatatcata tccactatct attttattag aacaataata
     7021 tatagaatct gatagatgta ttccatgttc tttacatgtt tttaaaaaag atttacccct
     7081 taaagtcata catgtaaagg aagttgggtt gaatacaaca gcaatatcat ttttaccatt
     7141 ttgaatagca tgtaaagctg ctatacgacc agtttcctca ttatcaatag atacacttga
     7201 aaaaccaggt atttcacgat ttacaacaac tacaggaatt ttaggtttat tttttactag
     7261 atattctata tcatcagcat tgatacctgt taaaacaatt ccatcataat aattatttgc
     7321 aaaaaagtat tcatcatcac atagatgacc aactttgtaa ggtcgtatga tgatatttat
     7381 gtttaaatgt tctaaagaaa ttatgtggtt tattgcttga agtatattgg ttgatgccaa
     7441 ctcaaatccc tcatcctgcc aataaaaagc tattttaata ttattatgtt taataggagt
     7501 agagcgttta tatccaagct tatcagctga atttaatata agctcttgtg tatgtagcgg
     7561 aatcctgcgt tcatttcctt taccacttaa gacgtacgag acagttgctg aagaaagacc
     7621 acagtctttt gcaatatctt tgattgttgc catgataaaa tcctccttaa atagttgctt
     7681 aaatttgctt ataccaataa tcttaacata tattcaacta aagaacaata ttctacaagg
     7741 tgcatatttt acaggattag aataaataaa tttaaaaaaa cttcttactt atgttgacat
     7801 aatttagttt ataggattat aatatgctta aataagctta ttattaactt gaattaagca
     7861 aagctaagca ggaacatgca atgctagctt gcaatgtgac tgtaaagggg gaaaaggtat
     7921 gagtaaggta aatatatttg aagttggtcc tagagatgga tttcaaaacc tgaaagagtt
     7981 tttagaaact gataaaaagc ttaagattat tgatggtctt attgcttcag ggattaaaca
     8041 acttgaaatt agttcatttg taagcccaaa agcaatacct cagatgaaag atgcaaaaga
     8101 agtagcaact tattgtgtag aaaaatatcc tgatgtgaaa ctttatgcat tagttccaaa
     8161 tttatacggt gctaaagttg catgggagtg tggtataaga gatatatcct atgttatatc
     8221 tttgagtgaa actcataata aaaaaaatat aaatcgtacc catgaagagt ctattgatga
     8281 gcttaaaaaa ataatgaata cttatccaga catgaatata tgtattggaa tggctactgt
     8341 atttggatgt ccttttgaag gtttaccaaa aattgaagat gccataagat ttgctaaacg
     8401 cctttgggaa aacggtatca gggagataaa tctagcagat acaataggga ttgctgaccc
     8461 taagcaagta agaaaaacta ttagtgcatt agttgaaact ttaccagagt gcagatggca
     8521 ggtacatatt catgatacta gaaatatggg aatggttaat actcttgtgg ctattgaatc
     8581 tggaattaca actgttcagt ctacacttgg aggattagga ggatgcccat ttgctcctgg
     8641 agcatctggc aatactgcaa ctgaagattt agtatatatg cttgaacgta tggggtatga
     8701 tacaggagtt gattttgtta aacttcttga attatcaaag tatcagaaga gttttgtaaa
     8761 tggagtatat agtggtcatc atgtaaatat aaagtctgat agttgtaatt gttagttcta
     8821 ttgattgatc gattaaaaaa atatgttgat taaaaaaata tgttgattaa aaaaatatgc
     8881 tgattggtaa aatagattta ttgataaaaa atataaatta taaaatacat tatctaataa
     8941 aacaaataaa aaaattgagg gggattagta tggatattat agctttactt ttatttttag
     9001 gagttattat tattgcattt gtacgtaaga ataatgcagg tattttggct ctagctattg
     9061 gtgtcatagc tgttagaatt tttggaatgg atgataaaga actttatgga gctataagca
     9121 actcaatgtt tactacactt gttggtataa cactactctt tgcaatagtg aactcaacag
     9181 gagcattaaa gcttgttgca gaaaaaattg tagctgcttc tggtaaacgt gtttggctta
     9241 tacctatagc agtttttata gcaggatttg ttgttgctgg tgttggacct ggggcaattc
     9301 cagcacttgc aattatccca gcaatggctg ttcctgtagc cataacagtt ggttataatc
     9361 ctgtaatgct tgcacttata ggtgaatgtg gattaatggc aggtcgtatg actcctatta
     9421 ctccagaagg tcaaattata acaactgttg cagaaggggt tggaattgaa aatgttatgc
     9481 ctactatctt agcctgtcaa actctttcta caattgtatt ttcaatagta ttgtttataa
     9541 ttttcaaagg atataaactt aaaaaaccta taaatgttat agatagtaag caaatagaaa
     9601 aattcaatat aaagcagata atttctctaa taagcatagt agttatgatt gcttttatca
     9661 taatttttga tatgaatgtt gggcttgcag cttttgcagt ttcagctgta cttatttttt
     9721 tcaatattgc tgatgaagga gaatgtatca aatcaattcc atggagcacg atagtaatgg
     9781 ttcttggagt aggtgcaatg atgacaatcg tagatgcagc aggaggtatt gacttgatga
     9841 gtaatgcatt atcatcactg atgaatagta aaacagctac tccaattatg agtatttcag
     9901 caggtcttat gtctatggtt agttctgctt tagcagtagt ttatccaact atgatgccta
     9961 tgtgtgttga tatagctaag gcagttggag gagtagaccc actagcactt attgctgcag
    10021 ttggtgttgg aggttctctt gctggtgtaa gcccactttc tactggtggg gcattgattt
    10081 tagcagctat gggttcaagt aagaaagact ttaataagga agaacaaaat aaggtttttg
    10141 tacagctttt cattatgagt gcagttggct tattggttat tgcagtagta tcaatattat
    10201 catttaatgc aataacacat ttaatgaact aattagaata actgaaaata aatcattaaa
    10261 tttaactttt aatattaatc aatataatat aatttggagg ggaagttatg aaaaaaggag
    10321 cattaagcaa tataaaggta cttgacctta caagagttct tgcaggtcca tattgtacaa
    10381 tgatgttggc ggattttgga gctgatgtta taaaaataga gatgccaaga aaaggtgatg
    10441 atacaagagg ttttggacct tttgtaaatg gagagagttt atactatgca aatataaata
    10501 gaaataaaaa aggtatcact ctaaacctta aatcagaaga aggaaaaaat atatttttag
    10561 aaatggtaaa acatgctgat gttgtagtag aaaattacag accaggtgta atggaaaagt
    10621 taggtctggg ttatgatgag ttggctaaag ttaacaatca aataatttat ggagctgtat
    10681 caggatttgg atgttatgga ccatattcag aacgtccagg ttatgatatt attgcacagg
    10741 caatgggtgg tcttatgagt ataacagggc aagaaggagg agaaccaacg agagttggta
    10801 atgctatggg tgatgtacta ggtggaatga atttaactat tggtatactc acagcactca
    10861 acgctagaaa aataatagga aaaggacaaa gagtagatgt ttccctcgtt gattctgttg
    10921 tatcgtctct cgaaactggt atacagagat actttgattc aggacaacct gaacttatgg
    10981 gtaatagata tgcttcagcc tatccttatg actcttttaa ggcaagtgat ggaagttttg
    11041 taataggctg tggtaatgac aaactttttg aaaagttatg taaaaaagtt ctttttagag
    11101 aagacctttt aatagatgag agatttaaaa caaatcttaa tcgttgtgaa cattacaagg
    11161 ctcttaaacc agaaatagaa aaatggtctg ttaagcatac aataaaagaa gcagttgact
    11221 taatatcaga ggctggagtt cctgctgctc ctatctataa tcttgattgt gttacaacag
    11281 atgaacatat agcaaaagca agagaaatga ttgtagatat tagtcatcct attataggaa
    11341 atatgaaagt caatggaaat ccagttaagt tgatgacaac aaaagctaaa attgatatgc
    11401 cagcaccatc tctaggggaa agtaacgagg agatttataa tggttggctt ggttttgata
    11461 gtgataaaat aaaagattta aaagaaaatg gagttatttg attaaaaaat agctaaacac
    11521 acttgtgact ttagtcatgg gaggttcagt tagaataata ccaaaaaata ttaaatcaat
    11581 actgtaaata aaatatagaa ttgtcagaag catttaagat gggaaaagct acttagacat
    11641 tgagtctttg tgcttttcct attttcatta agaattttat attatcagaa tagatatgta
    11701 ctaaactaaa gtattaacaa tttaaattaa tagaaaataa actacctaat tatgttgaat
    11761 aaagactact ttagtgaaaa taaacttata gagatgctat ttaaattatt ttaatggaaa
    11821 tatcaaaaat gagactgcat tctcaaaaat gagactgcgt tttttaagta ttttctcgaa
    11881 aatgagacaa aaatattgtt ttatgggttt tattgaagaa aaattcttct gtgaattaca
    11941 atttaatcaa ttttaaagtt gaaaattatt aaaaatttag tgaaatttat gtatatttcg
    12001 tttattttat taaaaataac aattttttac taaaaataaa gaacttcaat ggtgattggc
    12061 atagtacttg ctattttata ttaacaagaa atgtagaaaa aataacaatg tataatgggg
    12121 ggaatatgat ggaatctgta atggaaaatt tgaacaatct taaggtggag gttaaggaca
    12181 aagtctgtgt tattactata aatagaccta aagctttaaa tgctcttaat agtgacactc
    12241 ttagggaact atcacaagtt attgatgtag tatcagaaaa tgaggcaata ttaggagtta
    12301 ttattactgg agaaggtaaa gtttttgtag ccggtgctga tatacgccag atgcaaaact
    12361 ataaaagtga ggaaggcaga aaatatgcag gttatgctca gggaattttt gataaaattg
    12421 aagctcttga gaaaacagta atagcagcag ttaatggata tgcattaggt ggtggatgtg
    12481 aacttgccat gagttgtgat attagaattg catcagaaaa ggctatattt ggacaacctg
    12541 aagttaattt aggagttatt ccatgttttg gtggaactca aagattatca agacttgttg
    12601 gaactggaat agctaaagag ttaattttta cagggagaca agtaaatgca gaagaagcta
    12661 aatctattgg gcttattaat aaagtggttc caagtgattt acttttagaa gagtctatga
    12721 agatgatgaa ccaaatagtt gaaaaagcgc caatagcaat aagatatgca aaagttgtta
    12781 ttaacaaagg gatagatatg gatttaaaga atgctcttga actagagaaa gatattgcag
    12841 gtttaacttt tgctacaaga gataagcaag aaggaatgaa tgcatttata gaaaagagaa
    12901 agccagtttt tgaaaataag tagctattat aactaaaatt ggaacaatgt agaatatatt
    12961 caaaatctaa atttcgtgaa aaaatacata tagtttaatt ttaaaaggag ttaatttatg
    13021 aatacgggag taattataag tttagtaggg attatcatag ctctatctct tctagttgta
    13081 atggttatga agggaataaa tatttttatt atagcaattg tatgttcatc attagttgct
    13141 ataacaggaa gcctaaatgt gtatgatgca cttaaagtgg attatatgac agggtttgtt
    13201 ggatttcttc aggcaaattt ctttattttc ctaacaggga cattaatggg aaaaataatg
    13261 gagattactg gtggagcaaa atctattgct aagatgattg taagatggat tgggaaggat
    13321 aaagcactac tttctattcc aatagcttgt ggaatattag cctatggtgg agtaagtgtt
    13381 ttcgttgtaa gttttgcagt gtttccgata gctttagaaa tttttaaaga agctgatttg
    13441 ccacgaagat ttataccagc agctttgact tttggatgct caacttttgc tatggttgca
    13501 ccaggggcac cacaaattca gaatattgtt ccagcatcaa cattagggac agatattatg
    13561 gctggagcag taaatggttt tatatcatgt gcagtaatgt ttatagttgg aagtacaatt
    13621 ttgtacaaaa tggtttcaaa agaaaaagcc aatggtggtc attttatagc acatgaatca
    13681 gatatatttg atgaagcaga agcaactaat tctaagagag aaaatggtcc caatggtgta
    13741 attgccttaa ttccattgat agtgtcaatt ttaataatta atgtaaaagt taatggaaaa
    13801 gcgatagtac caattgaggt gggagtattt attggagctt tattagtata tttattgtta
    13861 aataaatatc aagataatgc taaaatagtt ggacatgttg gtgatgcatg taaaactaca
    13921 gtagtagcaa tatgtaatac ttgtgctgta gttgcatttg gttcagtagt aaaatcagca
    13981 gttggttttg acttcatagt taatgctatg actaatattc ctggtcctcc tattgcaggg
    14041 gctgcagttg gaactacagt tattgcaggt atttgtgggt cagcttcagg tggactagga
    14101 attgcagctc cacttcttgg accagtattt ttagccaagg gtgtttccgc tgcagcatta
    14161 actaggacta tggcaatttc atcagcagca ttagattcac ttccacacaa tggatatatt
    14221 gttactgtaa caaatggtct ttgtaaagaa acacataagg atgcctatat gccaatattc
    14281 tgggtaactg ttttaactcc gtttattgga accatagtag cagtcctact gtttacaatg
    14341 ttcccaggat tgccatagga gaaaagataa aaggtataaa ttatattttg atattataaa
    14401 atcaattttc aaaatatata caataaattt catgttttaa tactgtaaat atgtatttaa
    14461 aataacaaat tgaccatata aggaggaatt gaaagtgtta aataaaccat tagagggagt
    14521 taaggttatt gacctaacgt attttgtagc aggtccaggg acttctaaaa tattagctga
    14581 ctggggtgct gacgttatta aggtagagcc aagctttgga gacccaggaa gaaagacagg
    14641 ggcaacaatg acaatgccaa tagatgatta taataatcct ttttacagca catataattc
    14701 aaataaaaga ggtttgtcta taaatcttaa gagtgaaact ggaattgaaa ttatggataa
    14761 gcttttaagc gaagcaaacg tatttgtttc aagttataga acaggagcat taaaaagact
    14821 aggtcttgat tatgaatcat taagtaaaaa gcatccacac cttatctggg gtcagataaa
    14881 tggttttggc gattttggac ctgctaagga taatgcagga tttgatacag ttgccttttg
    14941 ggcaagaagt ggggctatgc ttgatatagc agaaaaagat acaagtccaa taaatccacc
    15001 tatagccttt ggagatgcaa caacttcttg tagtttatct ggaggaatat gtgcagcttt
    15061 gtaccagcaa gctaagactg gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc
    15121 aatatggaat gcatcatctt tattagcatc tacacagttt agtgatgagt atccaaaaac
    15181 tagaaaaaat gctatttcac cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat
    15241 attcttaagt attttagagc atgaaagata ttttgaggca ctttgtagat tatttggaag
    15301 agaagactta ataaaagaag agaaatttgc atcttcagta gaaagtaaga aaaatgctac
    15361 agaacttatt aatattttgg atggtgaatt tgcaaagttt actcaaaatg agatggttga
    15421 aaaacttact gctgctgata tagctcatga aaaaattcaa catgttaatg atgtagtgac
    15481 tgatgaacag gctattgcta ataactatat atatgaacat acaagtaaaa atggaaataa
    15541 gacaatgctt gcatctactc cagttaaatt tggaaatatt gaagttaaca tgacttgtga
    15601 tgctccactt ataggagaac attcagatga gatattgaga gaacttggat acagtgattc
    15661 tgatatagaa gctctaattg aatctggaat tgtaacaatg aataaagaag catgtgaaat
    15721 atgttaataa acatgtgaag tatgttaata aataggaggg aattttatgg attttagatt
    15781 aacagaagca caattaatgc ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc
    15841 accaatagct gctgaaacag acaaaacagg tatttttcca agggagcttt ttagcaaaat
    15901 ggctaaaata ggatttaatg gaattggaac acctgtagag tatagtggtt ctggtggggc
    15961 agatatagaa aaggtaattg ttgttactga gattgccaaa aaatgtgcag catctgcagc
    16021 aatattatca atacacacaa tatatgctca agctattttg aaatttggta ctgaggaaca
    16081 aaagaaaaaa taccttccaa tgatggctga aggtgggtgt gtaggtgctt ttgccttaac
    16141 cgaacctaat gctggttcag atgctgcaag agctgcaaca actgcaatta ttgatgaaga
    16201 aactgatgaa tatgttttaa atggaactaa gtgttttatt tctggtggag gtcaagcaga
    16261 atcattaata atatttgcac tgacagaccc atcaaaggga ataaagggaa tgtctgctat
    16321 cattgtcgac aaaggtactc caggtttttc aataggaaaa attgaagaaa aaatgggtat
    16381 tcatggttca gaaactgcag agttaatatt tgataactgt agagttccaa agtctaactt
    16441 attaggaaaa gaaggcaaag gatttaatat agctatgact tgtttagatg gagcaagaat
    16501 tggagttgga gctcaagctg taggaattgc tgaaggggct ttagaagaaa gtatcaaata
    16561 ttctaaagaa agagtccagt ttggcaagcc aatttcagct ttgcaaggta ttcaatggta
    16621 tatagcagat atggcgacaa tggtggaatc agcaaaatta ttagtatatt atgctgcaga
    16681 tttgaaagct agaggagaaa aacacactaa agaagctgct atggcaaaat acaatgcgtc
    16741 acgtactgca agagaggtta caaatttagc ccttcaaatc catggtggat atggatatat
    16801 gaaggattat ccattagaga gaatgtacag agatgccaaa ataacagaaa tttacgaagg
    16861 tacttcagaa atacacaaag ttgttatttc aagagcagta ctaggatagg aggaaaagaa
    16921 tgaatatagt tgtttgctta aagcaagttc cagatactaa tgaagttaaa ataaataaag
    16981 agacaggtac tctaataaga gatggtgtac caagtataat caatccagat gatagaaatg
    17041 ctttagaaga agctctaaaa atgaaagatg aactaggagc agtgattaag gttataagca
    17101 tgggacctcc tcaagctaaa agtgctttaa aagaagcttt ggcaatggga gctgatgaag
    17161 cttatttaat ctcagataga gcttttggtg gttcagatac ttgggctaca tctacaatta
    17221 ttgctgctgc tatagagaag gttggaaagt atgatgtaat attctgcgga agacaagcta
    17281 tagatggaga tacagctcag gttggaccag aggttgcaga atttttaggg ataccacaag
    17341 ttacttatgc gaaagaagta aaggttcaag atgacaaatt actagtaact cgttatacag
    17401 agacagggga ttatttaata gaagcaaaga tgccagtttt attaactgct ataaaagagt
    17461 taaacaatcc aagatatcca agtgtcaaag gaatcttaga agcttataat aatggagatg
    17521 caaagattac tgtattaaca cttgcagatt tagatgttga tacaactcaa attggattaa
    17581 agggttcacc tacaaacgtt tataagtctt ttgtaccagt taaagacaag cataatgaaa
    17641 taatagaagg tataaataaa aaagaaaaag ctgagaaact aatagaaata ctattcgatt
    17701 tgaaactagt ataggagtgt taagatgatg agagcaaaag ttaatcaagg tataaattta
    17761 aatgattata atggtgtttg ggtaatagga gagcaaaggg aaggtaaaat taatcctgtt
    17821 acgatagaac ttattggtga aggaagaaag cttgctgacc aattaggaaa agagcttgca
    17881 gttgtgatag ctggatatga agtagagaaa gaagtgaagg aattacttca ctatagtgtt
    17941 gataaagtat attacatcaa tgaccctctt cttaaagatt tcactactga tggatatgca
    18001 atttcaattg caaatttaat cgaaagaaaa aagccagagg ttgtattggt tggtgctact
    18061 tctataggaa gagatatagc gcctagaatt gcaggtaagg ttggaacagg tctaactgct
    18121 gactgtacaa aacttgaaat agattcaaca gataataagt tattacaaac aagaccagca
    18181 tttggtggaa atttaatggc cactattgtt tgtccaaaaa atagaccaca gatgtcaaca
    18241 gttcgtccag gagttatggc aaaagctgta agaaacgaat cagagactgg aattttagaa
    18301 gttgttactc cagaacttac tgaaaaaatg attagaacta gattagtaga gatacttcca
    18361 caagaaaaga aatctgtaaa tctaactgat gctagaataa tagtatcagg gggaagagga
    18421 ttaaaaagag cagaaggatt tgagcttata aaagaattgg ctgataaatt gggggcagag
    18481 attggtgctt caagagcagc agtagattca gggtggatag aacattctca tcaagtggga
    18541 caaacaggaa ctactgttag accagaacta tacattgcat gtggaatatc tggagcaata
    18601 caacatttag caggtatgag tgattctaaa tatatagttg ctataaataa agatgcaaaa
    18661 gcccctattt ttagtatatg tgactatgga atagtaggag atttatatga aattatacct
    18721 gaaatgatag aatcattaaa taggtaactt ttagaaaaca ttgaatatgt agaatgataa
    18781 gtcttataat attgtattgt aagacttatt atttttgctg aaggttatag tggggagatg
    18841 aaacaatgaa agagcagtgg tataaggata tatttgcaag agtattatct atgactgatg
    18901 atggttttat tgttgtaaat acaagtggag taataataga tattaatgat aaatattgtg
    18961 actttttagg aaaagaaaga aaagatataa taggacaaaa tatacaaagt attataccaa
    19021 atacaaaaat gctagatgtt atgaaaaata agtattgtga agaaggagct atacatcatt
    19081 attcaggtgg aaatacaaaa gaaaaaagtg taatagtaag taggtcttat gtggaaaatg
    19141 acaatggaga agtagttgca ggagtagctc aagttaaatt tagattgcaa tcatttgatg
    19201 tagctaaaaa gttaatgtca gaatatatgg agctccaata ttataaagag cagtttaagg
    19261 ataactgtgg ttttgacaag ctcataggtg aaaatagaga cttcatagaa cttaagaaaa
    19321 caggtgtgaa agcttctaaa acaaactttc cagtactttt aacgggtgaa acaggcacag
    19381 gtaaagaagt ttttgctaga gcaattcaca ataatagcag taggtcagat aaacctatgg
    19441 ttagtataaa ctgcgctgca ataccagaag aattattaga gtcagagcta tttggctatg
    19501 acgaaggagc ttttacagga gcaaaaaagg gtggtaaaaa aggtaaattt ttagttgcga
    19561 ataatggtac aatcttttta gacgagatag gtgacatgcc tttgaccatg caagcaaaac
    19621 ttttaagagt tcttcaggaa agtgagatag aaccagtggg aggtcttaaa actataaaga
    19681 tagatgtaag agtaatctct gccacaagaa agaatttaag taaaatggtt gaagagggat
    19741 tatttagaga agacctatac tatagattaa atgtaataaa cattcatatg atggaattga
    19801 aggacaggca agatgatatt ttattgctag caaattatat attaaacaaa ttaaatgttg
    19861 aatacaagga attgaaggtt ttaagtgata aggttaaaaa ttgttttatt aactatactt
    19921 ggccaggaaa tataagagaa ctacaaaatg taataaaaag tgcctatgct gtaagtgatg
    19981 atatggtcat tatgatgtgt gaccttccat ctaagatgga taatatcagc agagtagctc
    20041 aatgtaatgt ggatagcaat tgttcaattc atgaaatggt tgagaattat gaaaagagct
    20101 taattattga tgtgttaagg aagtataatt ggaaatgttc taaggcagca gaggttatgg
    20161 gaatacacaa gagtctttta tataagaaaa taaaaaagta tgaaattgaa ttaaataatt
    20221 aagataaatt gaattaaggc aatgctaaaa aagcgccctt attcaaagcg aactgtataa
    20281 actgttttat gcagttaaaa cgtgtaaaag tagatgtgaa gtactaatgt caatcattag
    20341 tactttttta ttatatttat aattactatc tatatttcag tatcttttga tatctaagat
    20401 tattatgttt aatttagtat ataacaaaac cctcttagtt atggacagtt ttttatattt
    20461 taactgtcta ctaagagggg atagtttagt ttaaatttag agtaaatttt atctagcatt
    20521 tataaaaaat attttgaata gcttttttgt tatttagtca taattttcta cttctttatc
    20581 aagttctttg ccacaagaat cacacttata tatagcattg tatccgtaat catattcatc
    20641 aactattttc ttttgtctaa atacaccacc acactcacat aggaaaccaa tttttataag
    20701 tgtaacgata aattgtgatt ttataaattt tatatcatta catttatcgt tagtgtaaaa
    20761 tgtataaata taagagttat cgcactcatc tcttaattca aatctttcaa tatatgtgtt
    20821 attttttaat tcctcaaatt cttctttagt taaatatgat ttttgttgca attcatgtaa
    20881 acgcttaatt tggtccatta tttatatcct cccaataatt ttagtatttt aagcttagat
    20941 aaggaatcta agttttatta aatttatacc aaggaattat ttaaatcata tttagtttta
    21001 ttaattacaa catatataaa ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt
    21061 gaaaacctaa actactaacc ccttactaac ccaaaaacta aaaaaagaaa atatatccaa
    21121 atataagtga tatataaata aagatgtttc atataaattt aactttaaat attctgacaa
    21181 taatatatat aaatatatta tagttaaaat ataccacata ttttgggtat tgtatactgg
    21241 aattagagta attaatttat tttttaacat acaaatgatt ttatgaaaaa aaacaagact
    21301 caaaaatttt tatattcttt gatagaataa aattgagagt aaatttattg aattttataa
    21361 aatttataag gggtgagtct aagatatgca tgaagataaa aatgatatat ttgaatcaat
    21421 agatactgag gattttgata ataaaacaga agaagaatgt ttagagctag taagaaaaaa
    21481 tggattgaat ttaatataca taaaaaatca aacagaagag atatgtctag aggctgttag
    21541 acaaaattgt aatgcaataa aatgtgttca gaatcagaca gaaaaaatat gtatagaagc
    21601 agttaaacaa gattggagaa tgttagaatt tgtggaagag ccaacagaag aaatttgcat
    21661 gttggctatt aaccaagatg gaacagcatt aaaatatata gaaaatcaga cagaggagtt
    21721 atgcttaaga gccgttgaaa aaaatggtgc agctttggag tatgtaaaag aacaaacaga
    21781 agaaatatgt atagaagcag ttagaaacag cgaatttgga ttggcaagag tgaaaaatca
    21841 aacagaaaaa atatgtatgg aagcagttaa gcattgtagc tataatctaa aatgggtaga
    21901 aaatcaaaca gaagaaatat gtatggaagc agtgagacaa aatgggcttg atttgaaatt
    21961 tgtaaaaaat caaacagaaa caatctgttt aagagcagta agacaaaatg gaatggcatt
    22021 agaatttgta aaggaacaga ctgttggtat atgcttaaag gcagtaagac aatatggaat
    22081 ggcattaaaa tttgtaaaag aacaaacaga agaaatttgt acagaagcta taaaacaaga
    22141 taaaagagca cttagttttg taaaaggaga taaggaaaaa tacaaggctc tatatgataa
    22201 taatgaacca tttgcaaaga gatatgttag aaatgtaata gaaaaagaaa acagagcatt
    22261 gattaaaaaa ggagaagaaa atgcaaaaat caaaattgca ggaaaacttt atgatagagg
    22321 catgtctttt gaagatatat cagatatagt tgagattgaa ataagcaagt taaaagtgag
    22381 tttaggtgtt ttttaaagta ataaatgtag gtgtttttta tgggaaatag ttaataaaaa
    22441 aataatcgac tagctctttg taggacagca aaattagtga tataatttga tgtataataa
    22501 attattttta ttagataagg gggaattaaa gttgaagatt acagtattat accaaacaag
    22561 aagtggaaac acagaaaggg tcgctaagct gatagaagat ggagctaaaa aggtggatgg
    22621 agtagatgta aagcttatga gacttgataa tattgattta gattatttaa atgaaagtaa
    22681 agcagtaata tttggaacac ctacttattt agcaaataca acttgggaag taaaaaaatg
    22741 gtttgatgaa gattcaaaaa aagttaattt agctggaaaa ttaggagctg tttttgctac
    22801 atgtgattat atatgtggag gtccagatgt tgcgatactt actatagtag gacatttgat
    22861 ggttaaaggg atgcttgtgt attctggagg tggttcttta ggaaagccat ttatacattt
    22921 aggacatgta cattcaattg aaggtccaga gttacaagat gagaaggcta taatatttgg
    22981 tgaaagaatt gctactaagg caaaagaact atttgcttaa tttctatatg catagtaatg
    23041 attagagtaa atttagggtt ttaagggaga tttgaagtaa aagttataaa ataaaaaatt
    23101 ttccataaaa ttactcaaga tgaattataa tataattata aataaattat attattaaaa
    23161 tatttaaaat caaacgtcaa tagcgaggga agtttatggt atggcatata gaatatgaga
    23221 ttttttctgc aattatagtt atttttttaa tggtatactt ttttagaagt aaatttatac
    23281 ctactttaca aaacaaaata tattgtgcac ttttaatatt ctcattttta tttataattt
    23341 caaatatttt aggttcattt tgtctaaata atatagacaa aattccaatt tttattactt
    23401 ttttattgaa tcagatatac ttattgttat tgccaatacc agcagcactt gtatcatttt
    23461 atgttatggc tattatttat caagatataa ggtatatgaa aaaaagatta ttatttttat
    23521 ctataccact tataatttgt ataatcttat ctattacaaa tccttttacg catattctat
    23581 ttggattgtc aaaagaaaca ggttatatac gaggttgtgg atatgttgca acttttgcat
    23641 cttttttctt ttatgcaata tattcagcat ttttatcttt taaatataga aaagcaatgc
    23701 atgaatcaaa aatatgggct attagaggtt ttcttatagt ttcaattgtg gcaattatct
    23761 tacaggcaat ttttttacag tatcttctta caggaactgc atgtgcatgt agtttacttt
    23821 tgacatatct ttctatgcag aatcgaggtc ttattattga tgaccttact ggtgtactca
    23881 atagacaatc ttttattcaa gagctagatt tgaatatcaa tactgaacaa tcaggattta
    23941 ttattacaat tgcgcttgat gattttaagt ttatgaatga aacttttgga acaaaaaatg
    24001 gagatattgc actaaaagaa gttggaaaat atttgattca gatacatgat gtaaaccatg
    24061 tatatagaat agggggagat atattttcta ttgttgtcaa tagtcaatta ggtatagaac
    24121 cagatgatat tatcagaaag atagaagaaa gatttaaaaa gccttggcta gtagaagaga
    24181 taagttttaa tttagcaact agtattgctg ttgtttatta cccagaaaat gcagaaacga
    24241 cagaagatgt aattgttgct atagatttct ctatacatga agcaaaacgt tctgagtcaa
    24301 ggcgagttgt ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa catgttatta
    24361 aaaattgttt aaaagaagca ttaaaaaatg atgggtttga agtgtactac caacctatat
    24421 tttctaatag caggggcagg tttacttcag cagaggcatt acttaggcta gaacacaaag
    24481 agttaggttt tatacctcct gatgaattta ttccaattgc agaacaaaca ggattgatta
    24541 actcaattgg attagtagtc tttgagaagg tatgtagatt tatagctagt gatgagtttg
    24601 aaaatttaaa attggataat atagctgtga atctttctgt agtacaatgt atgcaaaaaa
    24661 acttagctga tgatttactt tatcttatga ataaatataa tgtatcacca actaaattta
    24721 aacttgaaat tactgagact gtggctatgg gctcttttaa tataattaaa gaaacaatgg
    24781 aaagattaat tgatttaggt gttaagtttg cacttgatga ttttggaatt gcttattctg
    24841 gagttactaa tatgctttct ttaccatttt cagtaattaa gttagataag agtttgattt
    24901 gatctatgaa tgaagatagt aggcataaac ttacagttga gacgattata gcacttataa
    24961 ataaattgaa tatgaaggct gtagcagaag gtgttgaaac cattaagtgt gctgaagatt
    25021 taatagctat ggagtgtgaa tatttgcaag ggtattattt ttctaaacca gttccagaag
    25081 atgtttttaa aaatttgttg aaagaaaata cttttacaag ttaacaccct ttattttttt
    25141 aattacaact ttgttcattg acactgccag cgatatatta taaaattaac acaataataa
    25201 atattaaatt tattatacat attattaata ttaattaatg gagtaaagat ttattatata
    25261 ttttatttaa ttatcctaaa gtgagaaagg aagatgtgac atgaataaaa agaaaatatt
    25321 agtgccaatt gatgggacag aaagaagtat gcattctttg gaatttataa aaggaatatt
    25381 taaaaaagat gaagttgaag tagaaataat gaatgtaaaa gaactagtat ttattgatgg
    25441 catatccctg gctgaagaga taaaaaattc tgaaaattta ggaagaagaa tcttggataa
    25501 agcagcagag ataatgggag agtatgatgt taaagtacat tttacttttg gatatccagg
    25561 agatgagata attagaaaag caaaagaaga taatgtagat tttatagtta tgacaaaatc
    25621 tactaaaaaa ggtcttacaa gaatgatagg ttcagtaaca gcaagtgtag ttaaacaagc
    25681 aaaatgtata gtgatgattg taccggagta gaattagaaa ggtatttgaa atatgaataa
    25741 aaagatagta gcagtttgtg aaagtgaaag aagtttaaat tctcttaaga cagctgcaaa
    25801 tgatttagga tttgatttag tttatgaaat tcaagaaaat gataatataa aaaatgaact
    25861 ttctatttat gatatagaag atgcatccat agtgttattt gttacaagtg attctataga
    25921 aagcatagag aagattgata gatttataga tagagaattt tatgaagttg atccaaagta
    25981 tataatagag gatgcaaaat ctatagtaag tgaaatttta atagacttaa attaatcgtg
    26041 ggtgtcgcac taagaataga gcgacgcctt tttatatatt ttacacaaga aataaagagg
    26101 ttgtatcaaa atatagataa gactcttttt cattgttgaa atgatgaaaa tatatttgat
    26161 ttcatacaaa aaagagtacc tcataactaa aaagttcact ttgagacact ccatttttat
    26221 atgttagttg catttaaggt aaaatataag tttaatcatg ttgaaataac tgaattacat
    26281 cttctaaatg attgtacatt gatgtataac ctaagtaaga gtctttacta tcaaatgcca
    26341 tcattatctt gttatggtgg ctaatcatat ccttgagaga atttctatat ttaaaaccga
    26401 agtattggtt agaagaaact gttttaggta ttaactcata atattttata aaaattggat
    26461 tatgagaagc taaaattatc tctttgtgaa attgcaagtc agcatcataa cattcttcta
    26521 tcaaaccatg agaaacctta tccaattcct ctattgactt taggtaagta tattttattt
    26581 tttgaagttc tttagatgta gcatttaaaa ttgctagttt agtagcttca ctttcgataa
    26641 gttgtcttgc tcttaaagat tctacataaa attcttgtat gttatgattt ttatagattt
    26701 caccatcatc aaataggtca acttttttaa atccttcaac tataggactt gagtaaggca
    26761 cagctctaag tattccttcc ttacagagta tatttatggc atttcttacg ggtattctac
    26821 ttatgttaaa taggtcagca atctttctct cacttggtaa tttactatta attttaaaag
    26881 ttccatttag aatgtttaat ttaatataat caataatttt ttcagtggca tttaagcttt
    26941 tcataaaaac tcctaaacat ataatttttt tatgattgta ttatatcata gttaagagtt
    27001 aaatctactt ttttataata tgtataagac aggatagagg ccaaaatagc tataatagcc
    27061 aagataaagt atccatattg tattgaagta tattccgata ttttaccaac tatcattgga
    27121 aatacagaca ttcctatagc ataaattgct tgaaaaaaac ccattgcagt agatttctga
    27181 tgttttggta catttttcat agactcgcta gtaaggtaag aaaggagtat acctgtagat
    27241 atacctggta gtacttgtag tacaaatatt atattaatat tttttattgt tggaactaaa
    27301 aagcaatagg ctgaaactaa aataaaaact aaaggaatcc aaaattttgg cccatactta
    27361 gtacaaaatt tagtagaagc aaagccagaa gaagcaacag cacacaccat gtaaaatata
    27421 gaagatagac ctatgagggt agaactagaa cctaagtttt taagaatttg agtagtaaat
    27481 gacattgttg tagacatttg aataccctgt tgaattaatg ccataaagga aaaaaataca
    27541 agtcttttat ccttgcagat agataaatat tttttaattg atattttttc aggtattttt
    27601 tttgaattat gattttttat atttaaggct agaatcgttc caataactga agacaataca
    27661 ctcatgatac ataaggctgt cattcctaat ttttcataaa ataaagtact cataacaaat
    27721 ccaatcaaca ttcctagatt gtttaccaat attatcgtac ttgtagcttt ttgttgatgt
    27781 tctgtagaaa aaaagttggt atagtgaacc atgtaagaaa tccaagttga tgatgatatt
    27841 ccagataaaa tgttagctat aagaaaacca ttgcctccag gagaaaagac tcttataaga
    27901 gatgctaaac cagcaaagaa aattccagac agtataaata ttttatgttt atttatagaa
    27961 tcagcaaata ctccaacagg aagccttaat aaacattgtg atattccata tgcacctatt
    28021 attataccaa tcatatttgt agatacatta catgcactta aatatggatt ttggaaaggt
    28081 atatatacat attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag taatttttga
    28141 ttagatattt ttttcatatt atcctccgta tggtattttt ataatgcaaa taatatacca
    28201 tttagatttt ttaatcaagt actttctcta ttaaaaaaaa tttaaatata taattattat
    28261 ttacaaaaaa attatacttt atagttgaat taagaaaacg cttatgatac tataaagtta
    28321 acaaatttga atatataaca gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat
    28381 ttttatagat tatctataga gtactatggg tataatctat ttatttggtt cgattttttt
    28441 gtttaattgg ccataaaaca aaaagcctat agttgaaaat ggaggatttt ttatggatta
    28501 taaaaatata gctcaagaga ttctactaaa tgtaggagga aaagagaatg taaatgaagt
    28561 gactcattgt atgacaagac ttagatttaa agtcaaaagc gcttctaaag tgaataagga
    28621 taaattatct aaaactgaag gagtaataac tgttgttgag agtatgggtc aaatacaagt
    28681 tgtaattgga aataaagtaa aaaaagtata tgatgaggtt ataaaaattg tacctcaatc
    28741 aaataacata ggtaaaaaga atgaaagtca agaaaagcaa ggtataatta attctatatt
    28801 atcagcagta gctggtatat ttacacctac aatacctgct atagcagggg ttggtatgat
    28861 taaaggtatt ttatcagttc ttgctatgta ttatatgaat aaaaatggag tagatataaa
    28921 agaaacgcaa agctatatta tcttaaatgc tatggctgat tctatattct attttatgcc
    28981 tattatacta ggatatactg cggctaaagt atttaatgca aataaaataa tatctatggt
    29041 attaggagct acactttgtt atccaacttt tactgctttg atggcaggtg aagaatctgt
    29101 tagattttta ggattggcag taacaaaagc tacgtataca tcatctgtta ttccaataat
    29161 tattgctata tgggctcttt catacgttga aaaagtatta gaaaagtata tacctgaggt
    29221 aattaaaata ataatggttc caactttatc attggtagta atgttacctg caacattatt
    29281 tttatttgga ccaatcggaa tttatatagg aaatgtcata aacttttctt ataagtatat
    29341 ttatgaatta agcccagcat tgtgtggagc atttgtaggt gggttatggt gtgtgttggt
    29401 tatatttggt gctcatagag cattattacc aataggtata agtgatgttg ctcaaacagg
    29461 aagacagaat ctattggcat ttgcaggagc tgcaaacttt tcacaagcag gggctgctct
    29521 aggagtattt tttaaaacta aaaatcaagg actaaaaaca atttctatgt ctgcaactat
    29581 aacagcttta tttggtataa ctgaacctgc aatatacggt gccaatctta gattaaaaaa
    29641 acctatggta tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg gaatgggtgg
    29701 agcttatggt aatgcatttg caaaccaagg tgttttaact atacctgtat atgctgaagc
    29761 aggtgcatta ggtttcttaa gttatctagg tggttgtgct atagcatttt ttggttcagc
    29821 tatttcaact tatctagttg gatttgaaga tttggaagag tcttctaata aggaaaattc
    29881 atctataaaa gtagaaacga aggatggtgt tattgatata acttcacctg tagagggcga
    29941 atgtatagaa ttgtcagaag taaaggatga tgtatttgct tcaaaagcta tgggagaagg
    30001 tatagctgtg ttacctacaa aaggagtaat aacagcccct actgattgtg aagtagcttc
    30061 tttatttcca acattacatg caataggatt aaaattagat aatggagcag aaatgttaat
    30121 acatgttgga atcaatacag tagagttaaa tggtaaatac tttacaaaac atgttaatca
    30181 aggagattta gtgaaaaaag gtgataaact tatatccttt gatatagata aaataaaaaa
    30241 ggctggttat gatgtaacta cgccagttat agtaaacaat acatttgatt ttgggcaagt
    30301 agtttcttgt aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc
    30361 tggtaattaa gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat
    30421 caacaattaa atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta
    30481 atatggagta aagctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt
    30541 attgtaatga attgttaaag aaaataatta atatagggta aatctatttc aatgaaataa
    30601 caaagaaaag agttaatata gagtgaactt attgcaatga atcaacaaag aaaataatca
    30661 atataaaaag gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca
    30721 tcatcttctg catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta
    30781 gccgactata attcttttaa aaaatctcat ttacaggcag atactaaagt cgcatcagac
    30841 ttttaccata attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat
    30901 cgtttttcaa tttcatgggc aagaataata ccagatggtg aaggagagat aaaccagaaa
    30961 ggtttagact tttataataa gataatagac aaactaatag agtgtgatat tgagccattc
    31021 gtaactttat accattttga ccttccattt aaattagtag agaaatataa tggttgggaa
    31081 agtagagaaa ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga
    31141 gatagggtaa agtattggca acctcataat gaacagaatt taattgttag agtggaggag
    31201 agaattaata tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg
    31261 gattataatc tttgcttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa
    31321 gaaagtaaaa ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca
    31381 gaagatgtat atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg
    31441 catcattatg gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatatagtt
    31501 cctcacatgg aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct
    31561 gtaaattatt ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt
    31621 ggattaagag aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat
    31681 cctaacttag aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc
    31741 ttaaatgaat attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga
    31801 gctaaagata tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga
    31861 agtcatataa atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt
    31921 ccatggtcat ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt
    31981 gtttatataa atagaactga ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt
    32041 ttttactggt ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat
    32101 attgagggtt ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag
    32161 gatgataatg atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc
    32221 gggtctgagt taaataaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata
    32281 aaaaagagta ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa
    32341 atgataattg catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg
    32401 gccttaactg accacattgc ctttgcagtg agacgtttta aagaaggtat aatgattgaa
    32461 aatcactatt tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat
    32521 gctattactg tttttaagga ggttcttggt ttagacctac cagaagaaga gattggtaat
    32581 atagcaggcc attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga
    32641 agtgctaaaa ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat
    32701 aataaagagt cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga
    32761 tttctggcta atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa
    32821 aattgtaagg aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac
    32881 tccagagaat taccaagaca agaggagtta tatttgatga ttcatattca taagatttta
    32941 ggagaattat gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta
    33001 aaaattaact gcactttaaa tatgaataat tgcattactt aagggggttg tcttaaaatt
    33061 gattttttta attaaatttg aagctcaact ccttttttaa acctttatat cattacaact
    33121 ttaatgttta tacgactgta ctttagatgt atatctactt tttacatttc tcaacatgca
    33181 aacctaccat tttttgcagt atgttaattg aaataaacca ttgagttaac cctaaattta
    33241 ttctggactt ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt
    33301 atttcacata tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag
    33361 taaatgtatt atcatgtttg ttttattcaa gcacacttat aatacgcttg attttttcac
    33421 aatgatgaaa tatactaatg ggtgttatag gtataagaat cgcattacac ttgaagaagt
    33481 aagtgataaa tgagatataa atcctcatat gataaactac tatttcattg aggaacatat
    33541 tctaagtgat gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga
    33601 atgagtgtag aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat
    33661 ctttgtattt tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa ctattgccat
    33721 tctgggagag atggactggt tgaactttca aaacaagatt atcagttagt agttctagat
    33781 gtgatgatgc caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt
    33841 attcccatct taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga
    33901 gctggtgcag atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta
    33961 ctgtctttaa tccgacgtta cacccgtttt aatccaaaaa atgaggagcc acagcaactt
    34021 tctttcaaag gtttgttgat tgaccttgac cagcatacag ttactaccaa tggcgaacct
    34081 atgttgcttc cacccaagga atttggtgta cttctttact gtgcaaggca tcagggtaag
    34141 attttgacaa aacagcagat ttatgaagcc gtgtggggtg agccatatgt atatgatgac
    34201 agcaatatca tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct
    34261 cccatctatt tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa
    34321 tggatgaaca aacgataatt cttttcttac tcttgctgtc gattggtcta ctttttcttt
    34381 ccatatttgc tttttccaag cttgggcata tttacaagcg cctaaaagat atagaggaga
    34441 tactggctga tgtacaaatt ggagacgaaa atcgtaaaat actaattaag ccatgtgatg
    34501 ttatggctcc attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac
    34561 tttctctgaa aaagtcggat aaagctagca aacaacttat gacaagttta tctcatgatg
    34621 ttcgtacacc attaacaact ctgattggtt atctggatgc tgtacacagt ggtatagtta
    34681 tcgaacaaga gcgggaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg
    34741 attacattga tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa
    34801 tagaatctgt ggaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat
    34861 tccatgaaaa gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaacc
    34921 tcgacccaga tggatattcc cgtgtggtca ataatctggt tcaaaatgtt cttgctcaca
    34981 gtaaggctcg gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg
    35041 ttgaggatga tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt
    35101 ataagtgtga taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc
    35161 aaatggtgga gcgaatgggt ggtcaggttt ccgtagaaag tgagattgga aagtataccg
    35221 tatttacagt gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca
    35281 catggggatt ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta
    35341 atgtaagatt gaggtggtat gataacttta ataaacaaag gaggctttta ttatttatga
    35401 ataatttaat tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt
    35461 ctaatttgaa tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg
    35521 cgggtaagac gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa
    35581 ttaaaatatt tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat
    35641 gtttgattga atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct
    35701 ttgcaaggct tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg
    35761 gtttgccaaa caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt
    35821 tggcgattgc tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca
    35881 atggacttga tcctattggt attgcagaag taagaacgtt tattcgtgat ctctgcacag
    35941 aacgaggtaa aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag
    36001 atgacattgg cattattgac catggtattt tgttggagga agaaagtctt gcccaattag
    36061 aagaaaaaaa cagtaaatac atacattttg ctgtatctga tgcagcacag gcagcaaggc
    36121 ttctggaaac ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc
    36181 tatatgatac agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg
    36241 aggtatcaga atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag
    36301 ggggtgaagg aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa
    36361 tttatactga tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt
    36421 gcaaaggaca acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt
    36481 ttgctattac caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat
    36541 aatgatgtat taaaaaatct tgcaacagtg ccagtgcaaa aaggaaaact ggctctggca
    36601 aaattggctg tgttgctgtt tatatcattg ttctactctg ttgcagggtt aggagctact
    36661 atcattggag gtttcattgt tggaatagta gagggggttg ctgtaaaatt aggaatgagc
    36721 attgcttttg gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat
    36781 ttcaatcgca gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt
    36841 agtatatcct taaatattat caattttgaa ccaaataacc caatattaag tgtattacct
    36901 gctcctgtta taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg
    36961 gcacttagac agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata
    37021 accattatag cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta
    37081 tctatgcttg atataattac tacagaattt caaaaaataa aaagatataa tattctatgg
    37141 attggtgttg ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca
    37201 cacggcagtg accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc
    37261 agtcttagtt ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat
    37321 acagaccaaa ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg
    37381 ggaaaattaa ttgcggttgg aggaattact gtattgttaa gtcttttcag cttcttatgc
    37441 acatttattt tggggttgtt gttttgcaat atcaatatat ctgttacact cgttgtaaaa
    37501 tccttagttc agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt
    37561 gcttattttg gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac
    37621 ggtttttgtg gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca
    37681 ggtcttggtc tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt
    37741 gctatgactt tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat
    37801 tatgataaag ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat
    37861 aaataacata aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg
    37921 tcgtacagtg tctatatgct catgtattca agaagctaca gttttgattt ttcataactg
    37981 tagcttcttt ttgtatttat taaatcacga ctatcataat acttgcaata ggtatagcga
    38041 caagagagag tactgtcata tgtgttttat atattacatt aagtatattc taaaatatat
    38101 gttttaaata atatatttga aaataggaat atttaaataa aaaaaataat attatatata
    38161 gttgacaaaa attggcaaat gagataatat ataaatatag aaatagtaat cattactatt
    38221 attaaaaagg gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatttaat
    38281 ggcaggattt gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc
    38341 aagaaaagaa ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa
    38401 ggaacatgct aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa
    38461 tttaaaggat gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc
    38521 taaagaagct agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa
    38581 aatagagaaa gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa
    38641 aatatttaaa agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt
    38701 aggtactgaa gcaccagaaa aatgcccagt atgtgaccat ccaaaagcat atttcaatat
    38761 aaaagctgaa aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt
    38821 ctaaacaacg agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg
    38881 attacctata tgaaaactta aaaaaagata atccaaattt aagcttagga actgtgtata
    38941 gaaatttagc tcagctaacg gaacatggct ttataagaaa ggttagtatt ccagggtatc
    39001 cagatagatt tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag
    39061 aggtatacga tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga
    39121 cagacataaa aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa
    39181 ggtgtagcca agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt
    39241 gtagtgaaca aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa
    39301 gtggtggtgt acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa
    39361 cagatgcagc agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg
    39421 ttaaagtaag tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc
    39481 ttatgactga acaaggtggt caacgcaaat gtctagcagt agatggagaa cctgtagtta
    39541 aatttgcttt gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg
    39601 gattatggaa ggctgaactg taatatttaa ataaattgag tcaaaaagga ggctataaat
    39661 gtattttcat acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta
    39721 atgtcaatat aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat
    39781 atatttatga tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat
    39841 gccctgtatg tacaaaaggt cgtagctatt ttggaaagat aagtactgtt tactatgaag
    39901 aagatgaaaa gatagcagaa gacatagttg aagatgaatc taaattaaat actgaaaaag
    39961 agggagattt aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata
    40021 tggatataat tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacga
    40081 aactaccagt aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac
    40141 ctttaaatga gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac
    40201 ctatgattat agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag
    40261 aactaaaaat agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg
    40321 aaggaggtat attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac
    40381 caaataaata tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa
    40441 ttggtcaagg aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag
    40501 aaatagcaaa agtaagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg
    40561 aggaaataca atccaaagag gatttaaaga aacttgtaga tgaacttaga gaagtttcag
    40621 aagggcgtcc aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta
    40681 tagcttatgc taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa
    40741 gtccaaagtt gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta
    40801 gaaaatacat agatacacat ggattagata tagatttggt tataacaggt gggcttcgta
    40861 tatctactga ttttgctaag gcaattgcta tgggtgctga tgcagttgct attgcaagtt
    40921 ctgctcttat ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg
    40981 taggagtagc tactcaggat gaggaattga gaaaaagatt acatatagaa aacagtgcta
    41041 atagagtggc aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt
    41101 ctggtcataa agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa
    41161 tatcaaatta tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag
    41221 atagctataa aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt
    41281 tttagttcat aagtcactct tttttttatg aagccaaata tatttatcat tttagcaatg
    41341 aaaaagaatc ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat
    41401 attaaattgt aaaatattct gtgatgtcgt actattaaac taaagattct gtactttaaa
    41461 gtaaataaaa atttaaggag tgattattat ggaaagattg aagatacaag ttttagttga
    41521 caataatact tatattgata gatactttgt tggagaacca gcagtaagct attatattga
    41581 gattgatgga aataggattt tgtttgactc agggtattca gatgtattca tatcaaacgc
    41641 tgaaaaatta aatatagatt taggaaattt aactcatgtt gttttttctc atgggcataa
    41701 tgaccataca agaggtatcc agtttctaga aaataggtat gatttatcta cagttgaact
    41761 tatttcacat cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc
    41821 attttctgct gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa
    41881 tctaagtaag aattgtgtgt ttttaggtga aataccttct ataaatgatt ttgaaaaaag
    41941 agctaaaatt ggtaaatgta aaataggaga tttgtgggaa gatgattatg ttttagatga
    42001 ttctgctata gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag
    42061 tggaatttgt aatatagtag agtatgctaa aaaggtatgt ggagatgata gagttattgg
    42121 tatactaggt ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata
    42181 tttggataaa gaagaaatga gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa
    42241 agcgaagatg aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat
    42301 atagtatttg aaagtatata agatttaagt tgaaggttaa aaagatattt tctatagaaa
    42361 atatcttttt tgttacgttt attttaagag aaatcaatat acttttatat gaaatattgt
    42421 aaaaaaattt atttttaatg agaatatatt ttcattaata aacaatttta ttatgtatta
    42481 ataatgttag aataaatata aataaatcaa agttacttac attacagtat attttttcac
    42541 aaaatatatt gatttattct tttaaagttt gataattcc
//
LOCUS       DAELJO010000011        38512 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELJO010000011 DAELJO010000000
VERSION     DAELJO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.011"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..306)
                     /locus_tag="KPB88_000546"
     CDS             complement(<1..306)
                     /locus_tag="KPB88_000546"
                     /inference="COORDINATES: protein motif:HMM:NF024671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HBG0052854.1"
                     /translation="MSYKVMEIAQHVINYSIEQNAPVTNLKLQKLLYYIQAAFLVEFK
                     EKCFDEEIVHWRHGPVVYKVYSEYKIYTDEEIKDRQLESSDIIMNTNFNFSVEKIEYD
                     "
     gene            807..1484
                     /locus_tag="KPB88_000547"
     CDS             807..1484
                     /locus_tag="KPB88_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0052855.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            1653..3689
                     /locus_tag="KPB88_000548"
     CDS             1653..3689
                     /locus_tag="KPB88_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG0052856.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3880..4446)
                     /locus_tag="KPB88_000549"
     CDS             complement(3880..4446)
                     /locus_tag="KPB88_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0052857.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            4734..5669
                     /locus_tag="KPB88_000550"
     CDS             4734..5669
                     /locus_tag="KPB88_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0052858.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            6142..7356
                     /locus_tag="KPB88_000551"
     CDS             6142..7356
                     /locus_tag="KPB88_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052859.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREELIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(7705..8226)
                     /locus_tag="KPB88_000552"
     CDS             complement(7705..8226)
                     /locus_tag="KPB88_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0052860.1"
                     /translation="MKFKRLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            8582..9379
                     /locus_tag="KPB88_000553"
     CDS             8582..9379
                     /locus_tag="KPB88_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0052861.1"
                     /translation="MEKREKRIIISSLLSVSILMGLTSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            9489..10079
                     /locus_tag="KPB88_000554"
     CDS             9489..10079
                     /locus_tag="KPB88_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG0052862.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            10145..11842
                     /locus_tag="KPB88_000555"
     CDS             10145..11842
                     /locus_tag="KPB88_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0052863.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            12056..12895
                     /locus_tag="KPB88_000556"
     CDS             12056..12895
                     /locus_tag="KPB88_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0052864.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            13412..15268
                     /locus_tag="KPB88_000557"
     CDS             13412..15268
                     /locus_tag="KPB88_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0052865.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    15351..15591
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    15657..15892
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15977..17194
                     /locus_tag="KPB88_000558"
     CDS             15977..17194
                     /locus_tag="KPB88_000558"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0052866.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            17311..17793
                     /gene="cdeM"
                     /locus_tag="KPB88_000559"
     CDS             17311..17793
                     /gene="cdeM"
                     /locus_tag="KPB88_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG0052867.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            18057..19322
                     /gene="hisD"
                     /locus_tag="KPB88_000560"
     CDS             18057..19322
                     /gene="hisD"
                     /locus_tag="KPB88_000560"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG0052868.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRYEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(19525..20277)
                     /locus_tag="KPB88_000561"
     CDS             complement(19525..20277)
                     /locus_tag="KPB88_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0052869.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(20395..20664)
                     /locus_tag="KPB88_000562"
     CDS             complement(20395..20664)
                     /locus_tag="KPB88_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052870.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            21143..22237
                     /locus_tag="KPB88_000563"
     CDS             21143..22237
                     /locus_tag="KPB88_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0052871.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            22239..23189
                     /locus_tag="KPB88_000564"
     CDS             22239..23189
                     /locus_tag="KPB88_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG0052872.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            23182..24366
                     /locus_tag="KPB88_000565"
     CDS             23182..24366
                     /locus_tag="KPB88_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052873.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYIKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            24379..25875
                     /locus_tag="KPB88_000566"
     CDS             24379..25875
                     /locus_tag="KPB88_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0052874.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            25898..26848
                     /locus_tag="KPB88_000567"
     CDS             25898..26848
                     /locus_tag="KPB88_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0052875.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            26869..27831
                     /locus_tag="KPB88_000568"
     CDS             26869..27831
                     /locus_tag="KPB88_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0052876.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVGVNKDNISEFKGEW"
     gene            28203..28835
                     /locus_tag="KPB88_000569"
     CDS             28203..28835
                     /locus_tag="KPB88_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0052877.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            29246..29338
                     /gene="kdpF"
                     /locus_tag="KPB88_000570"
     CDS             29246..29338
                     /gene="kdpF"
                     /locus_tag="KPB88_000570"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG0052878.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            29379..31061
                     /gene="kdpA"
                     /locus_tag="KPB88_000571"
     CDS             29379..31061
                     /gene="kdpA"
                     /locus_tag="KPB88_000571"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG0052879.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            31086..33143
                     /gene="kdpB"
                     /locus_tag="KPB88_000572"
     CDS             31086..33143
                     /gene="kdpB"
                     /locus_tag="KPB88_000572"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG0052880.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            33162..33788
                     /locus_tag="KPB88_000573"
     CDS             33162..33788
                     /locus_tag="KPB88_000573"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG0052881.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            34025..34933
                     /gene="cysK"
                     /locus_tag="KPB88_000574"
     CDS             34025..34933
                     /gene="cysK"
                     /locus_tag="KPB88_000574"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG0052882.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            34960..35550
                     /gene="cysE"
                     /locus_tag="KPB88_000575"
     CDS             34960..35550
                     /gene="cysE"
                     /locus_tag="KPB88_000575"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG0052883.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            35710..35898
                     /locus_tag="KPB88_000576"
     CDS             35710..35898
                     /locus_tag="KPB88_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0052884.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(35952..36821)
                     /locus_tag="KPB88_000577"
     CDS             complement(35952..36821)
                     /locus_tag="KPB88_000577"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG0052885.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(37018..38262)
                     /locus_tag="KPB88_000578"
     CDS             complement(37018..38262)
                     /locus_tag="KPB88_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052886.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT        14957 a         4048 c         6681 g        12826 t
ORIGIN      
        1 atcgtattct attttttcaa cagaaaaatt gaagttagta ttcataatga tatctgaaga
       61 ttctaattgt ctgtctttta tttcttcatc tgtataaatt ttatattctg aataaacttt
      121 atagactaca gggccatgtc tccaatgcac tatttcttca tcaaaacatt tttctttgaa
      181 ctcaactaaa aaagcagctt gaatataata caatagtttt tgtagtttta aatttgtgac
      241 aggagcattt tgctctattg aataattaat aacatgttgt gctatttcca taactttata
      301 gctcataact acacctcctt agcagtaaac aaaacatatg ttttatttat tagtatactc
      361 atattgtata cttttaagtc aaaaatttca acaaatttga agaaaaatta tccaaaatta
      421 agatgaaaca taagaaattt aatttgttaa aatattatta acatttaaat gagtttattc
      481 ttaaaatatt aattatttaa atcacatata taaaaactaa ttatgaatat ttagtaaaaa
      541 tgaagtttat aaagttagtt ttctatattt tttacaaata aaaataaatg gtgacaaaac
      601 caaacttgcc accattttgc caccgaaaat aaataataga ataaatttaa attaaacata
      661 ataaaacata aacaacaaaa ctgaccttaa taaaacattg aaataccaac acttgtaaaa
      721 aataacaaca ataaacaaat aacctagaat aatattacaa cttaagtaaa taataatttt
      781 gaataataat ttacggaggt attcatatgt ttaaacacga taaagcatta ttaaaagaag
      841 taaaagtaga aagaccaaat cctcaatatg ctgttttaat gcaagaacag ttgggtggtg
      901 caaatggaga gttaaaagca gctatgcaat acttgtccca aagttttaga ataaaagacc
      961 ctcaaataaa agatttattt cttgatatag cagcagaaga gcttagccat atggaaatgg
     1021 tagcacaaac tataaattta ttaaatggac atgatgttga ttataatgca gttaatacag
     1081 gcgaaataga aacacatgta ttaactggtc tatcaccagt cttaataaac tcatcaggag
     1141 ctccttggac tgctaattac gttacagtta caggggattt agtagctgac ttactttcaa
     1201 atatagcttc tgaacaaaga gctaaggtag tttatgagta cttgtataga caaatagatg
     1261 ataaatatgt aaaagaaaca atagatttct tacttaatag agaagaagca cataatgctc
     1321 tatttagaga tgctttaaat aaagttaaag atacaggttc aaatagagat tttggagtta
     1381 cagaagattc taaattatat ttcgatttgt ctactccagg gccaaatcac gatactaaga
     1441 tagatattaa tcctccttct tttgaaaaac ctttaaaaaa ataatttgat attttttatt
     1501 ttgttttagt agttataaaa attatttact agaagaatta attatatgaa aaattaaata
     1561 tgtatagtta aaaatgggta tacacttatt ataagtgtat atctatttgt ttattgcaaa
     1621 acatataata aaataacaaa tgaggtggta ttttggacag taatagaaaa cctaataatc
     1681 taataaatga aaaatctcca tatcttttac agcatgctta taatcccata aactggtata
     1741 gttggaatga tgaggctttt aaaaaagcaa aagaagagga taaaccaata tttttaagtg
     1801 taggatattc aacatgtcat tggtgccatg ttatggaaaa agaatctttt gaagatgaag
     1861 aagttgctga aataatgaat agaaactttg tggcaataaa agtagataaa gaggaaagac
     1921 ctgatgtaga tagtgtgtat atgacagttt gccaagctat gacaggtagt ggtggatggc
     1981 caatgaccat aataatgaca ccagataaaa aacctttttt tgcgggaaca tatttcccaa
     2041 aatattcaag gtataataga cctggagtta tagatttatt agagaatgta tctgaaaagt
     2101 ggaatacaag cagagatata ttaataaaat ctggagatga aataatagaa gctcttaagg
     2161 atgattttgg tgtaaaaaat actgagggtg atttatcaaa agagatgttg agttcatcag
     2221 ttagagtatt taaggctatt tatgatgaaa agtatggtgg ttttggtaat gctcctaaat
     2281 ttccaagtcc tcagaatctt atgtttttaa tgaaatatta tagtatagaa aaagacaaag
     2341 atgtgttaaa aatggtggaa aaaactttag atggaatgta tagaggaggc ttgtttgatc
     2401 atattggatt tgggttttca agatactcta ctgataaaaa gtggttggct cctcattttg
     2461 aaaaaatgct atatgataat gctatgctta ctatagcttt tttagatgcg tataaaataa
     2521 caaagaaaga gttgtataaa gaaattgcta taaaaactat agattatgta gttagagaga
     2581 tgaaggataa agaaggtggg ttttattcag cacaggatgc tgatagtgaa ggtgaagaag
     2641 gtaaatttta tacatttaat cctcttgaga taattgaagt tttgggtgaa gaagatggaa
     2701 ctttctttaa taattatttt gatataacta gtagtggaaa ttttgaaggc aaaagtattc
     2761 ctaatcttat aaaaaataaa gaatatgaga gacataatga aaagatagct gaccttagta
     2821 aaaaggtatt tgaatataga aaagaaagaa catctttgca taaagatgac aagatactta
     2881 catcatggaa tgcattgatg atagttgctt taacaaaggc atatagtact ttaaaaaatg
     2941 acatatattt agagtattca aataagtgtc ttaactttat aaataataat cttgtaaatg
     3001 agtcaggtag attattagct agatatagag atggaagttc cgactattta gcatatttag
     3061 atgattatgc ttttttaatc tgggcgtata tagagcttta tgagtcaaca tttaatatga
     3121 aatatttaga aaaggcttta aatttgaatg aaagctgtat caaccttttt tgggattatg
     3181 aaaaaagtgg attttatata tatggtaaag atagtgaaaa tttaatagca agaccaaaag
     3241 atttgtatga tggagctatt ccatcaggaa attctgttca attatacaat cttataagac
     3301 ttgcgaaaat tactggagat aatagacttg aagaaatgtc atataaacaa ttaaaactgt
     3361 atgtggataa tgtaaagagt tctccaactg gctacagctt ttatatgctt tcattgatgt
     3421 ttgaacttta ttctactaag gaaattatat gtatttttaa ggaagattct gatttaattg
     3481 catttaaaga attgataagt gaaaatttta taccaaatgc aactttttta gcaaagaaat
     3541 acaatgaaga aaacactatt ataggttttt taaataacta tatattgaag gatgataaga
     3601 ctagttatta cgtgtgtcaa agtaatagtt gtagtcaacc aattaatgat ttacaaaaat
     3661 taaaagatat gattttagga gtagaataat cataatagcg aaaaaggaga tgctttaaaa
     3721 taaaagtcta atcatctcct ttttttgtat aaaaaacaga aaaagagttc ctaagaacac
     3781 ctaatctatg taatgattgt tttgttaaaa aataaaaatg acattatagt tatataaaat
     3841 attgtactat aaaaaagtgg tattatagat gcgatagtat taatcttttt tatttactct
     3901 atttcttctt tttgcaaatt cttcaagtgc ttcagattta attttatcta tttcttcata
     3961 ttccataaaa ttagcatctt cttcaaagcc atcttgttct tcaccattaa aacgatacat
     4021 tttttttagt aaactccata aggtctctaa ttcatctttg gtaaattcgt gaaatacatc
     4081 tgccataaag tttattccag tcctactaca tgtcaccata accttttttc ctaagtctgt
     4141 aactttaaca ttaattgctc tcttatcatg aggacttgga attacatcaa catatccatt
     4201 tttttcaaga ttagcaacta gtctatttat attttgttta cttgtaccca tttttcttgc
     4261 aatattattt aatgttgttt cttcctctgg taaatgaaga attgataaaa tagtcatata
     4321 ctgtcttgaa gtaagtatac caaaatattt atctccttct atttgtattt tgtttgtaag
     4381 ggtaaaaaga gttgcatatg tttgattcat taaaaagagc tcttttagtt catttgaata
     4441 atccatgaaa tatatctcct ttatttttag atgttgtttt gtcgaaaaca gaatatcatg
     4501 aattaattct aatgtcaata tggtgtcaaa atatattaaa gtattacttt tttatgtaac
     4561 tattaattaa ttaaattatt gtataatttt atgtaaaaac ttctatgtac caatgataac
     4621 ttctatgagg aattaataat tcttaaattt tttagaaaga ttttgacttg aaaatacttg
     4681 taatataata tataaattaa tatactgaat aaaatcaatc tggagggata attatgaagt
     4741 gtacaaactt tactttcaaa ggagaggaag gtttagatat atatacatat aaatgggaag
     4801 atgaaaatat aaaaaaacca aaggcagtta tccaaattgc acatggaatg gctgaaactg
     4861 cacaaaggta tgagactttt gccaaggtac ttactaaaaa tggttatata gtatatatca
     4921 atgaccatag aggtcatgga aaaactgcaa agataataga aaatgttgga catttagctg
     4981 aaaaagaagg atttagatgt cttgtagagg acatgtatac tttaacaaat attataaaga
     5041 aagaaaatga agatttgcca atttatctat ttggacacag catgggttca tttgcaagtc
     5101 aaagatatat aatggattac agtaataatt tatctggtct tatactatgt ggttcaaatg
     5161 gaaagcaagg aatcattcta aatcttgctc atctgataat caatcgtgag attaaaaagt
     5221 atggaagacg ttctaaaagt aataaaataa ataatttgat atttggcggc gaaataataa
     5281 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
     5341 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
     5401 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
     5461 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
     5521 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
     5581 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
     5641 ggttaaataa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa
     5701 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
     5761 cccagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
     5821 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
     5881 tgaaagttta aaagtttaaa tatagtaagt tgtaataaag agaaaatcta aatatttaaa
     5941 tgctatttaa tgtgaatcta ttaattaggt tatacttaaa cttaattaat agattttata
     6001 tttcaagaca atgagatagg gttttagatt aaaatataac caatggttaa attacagaca
     6061 ttttgttaac tttccctcga tttactagta aatatttttt ttatatactt aaattacaac
     6121 agatgtacta gggaggtata tatgcaaatt aaaataggta aagtcataca gcgtcttaga
     6181 aaagaacgaa atttaactca agaacaatta gcaaagttta taggtgtgtc aacaccagca
     6241 gtatctaagt gggagagtgg aaattcttac ccagatatag aattattgcc tttattagca
     6301 gactttttta atgtgtctat tgataagctt ttaaattaca agattgattt aagtgaagaa
     6361 gaagtaatga aaatttataa agaattagaa tcaggctttg ctagaattga aattgattta
     6421 tcaacagaag aaccaaaaga agaatttaga caagacttag aatctgtaaa aaagctttcc
     6481 aatatgtata tagagaagta tcctaaaagc tatttattga agctaaggat atgttcgctg
     6541 tatcagatgt attcatataa atttggtaag agtgaactta atgacagagt aaaagaaaca
     6601 acgaatattc ttgaagatat agtgagaaat acagatgata tccaaataaa agaaacagct
     6661 cttattatat tatcaaacgc atattgtatg ttggaggatt atgaaaaggc agagttgtat
     6721 ttaaatatga tacataagtc aataggggat acaagtgtta atttagctat gatatattta
     6781 aagcaaaata gacttgaaga agcagaaata ttgcttcaaa ataaattgtt tagtaatgta
     6841 tttaatataa gcatggattg taagggaata attaatgtat acaaaaatca atacaaggaa
     6901 ttaaagaaaa aattggagaa tagaaatttt aataaaaatg caattgagag agaaatggag
     6961 tatataaaaa ataagttgct ggggtatgca aatctttctt tagaaataaa aaaaatgctc
     7021 agtgaagata aaggtgcatt ttttagtatg tatatggatt atatggaatt atctctaatt
     7081 tttttatttt ttaacatgaa agaagaagca aaaaaagcct tatatagtct aaaagaaata
     7141 ttagaaaaat atcctataca tgaaagtttg gatgtaagtc aaatgagatt ttttgataag
     7201 gtagagtcta aaaatttata tacttttaat atatatacaa acttgttaat tgtacttaat
     7261 gatgatagtt ataatgagct tagagaagaa ctaattttta aggatgtaat tgaaaagatt
     7321 ttagatatgg aaaaaatgtt gaaaaataaa gaataagtaa aaatttaata taggatttaa
     7381 agattaatca aattaaaaga aagtgtttca aaataaaaat ttgaattacc ttttttgtcg
     7441 gtcttaaaca gttatttaga aaaatagaat aaagtttgtt atataaaatg taattttgtc
     7501 tataaaatct cacctgtttt cttaacttta agcgtgaaaa agaggatgta tcatgaattt
     7561 tttagttcat gatgcatcct tctttgtatg aaatccaata tatctttatt atttaaacaa
     7621 tggaaaagag tcttatttct attttgagac atctctttta tttaattatg aagttttata
     7681 cagttcaata aataggaatt atatttagaa actcatgaca ttatttctta gatagtgatt
     7741 aaatttatca tattccattt tagataactt ttctgggtaa gacattacaa tcaggttatg
     7801 cccattaaat ttttcatggt atggtggatg aatgtgttca taattatttg tcttatattt
     7861 tactctttca tagaacgcct tgcgataaat tgaaatctca tcaattggaa ggtcaatttc
     7921 taaaataata gtttttcctt tcttatttaa aagctctaac gctctttgtc catatctatt
     7981 attgcgcatt tctggaaaaa tgcagaaatg ttctacatag ataaagtcat ctgtttccca
     8041 acataaaatt atgccgacaa actggttttt atcataaatt aaattgaatt gatattcttt
     8101 atttttcaaa atttcttctt gcaaagaaga ttttctttgt tcatgaaatg ggaaactaat
     8161 tttatataat tccatagcct tgtaatataa tttattttca ctggttctta accgtttaaa
     8221 tttcacttta ctcctcctaa tcactaaaat tccaatttat ttatgagttc attttaccat
     8281 agtcagtatt tgcatacaat gtgttttaag ttgagtgtca tttctttctt ttatttatgt
     8341 ttatatctca tagactaaga gataattgct taagacataa ctaaaaacta ttatattaag
     8401 ctttgataat ctaaaaacag tatatttatg gacttttcta ggtctaaaag taatcttaat
     8461 aattttagaa gttacaaaaa agttacataa atttgggaag ctataatata catatataat
     8521 ataaggttaa aactgtaatg aataatatta atttaaaatt tgaacatgga aggagattat
     8581 tatggagaaa agagaaaaga ggataataat atcatcttta ttaagtgtat caattttaat
     8641 gggtttgaca agtatatatt ctatattaaa taaagaagac ataattttaa ctgttaaggg
     8701 tcaagagcaa aaagtatctt cctttaagaa aacagttgag gaacttttgg atgaacaggg
     8761 tgtaaagtat aattctgaag ataagattaa tccaagttta gatacagaac taaaagatga
     8821 tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa gaagagattg aaaaaattcc
     8881 atttgataca aagcatgtaa atgatagtaa tttgttaaaa ggaaaatcta aagtttatca
     8941 agaaggtcaa gaaggagaaa aaaaactagt ctataattta acttaccatg atggaaagtt
     9001 agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag ccaactacaa aaattataaa
     9061 atatggaact aaagaaaaag tactaatagc atcaagagga gcaaatataa gaggaggcaa
     9121 acatatgaaa gtggttgcca ctgcatatgc aggagataca ataacatcta ctggtacaac
     9181 tccaagatgg ggtgttattg cagttgaccc acgtgtaata ccatatggaa caaaagtata
     9241 tatacctaaa ctgggtatga cttttgtagc agaagattgt ggaggtgcaa ttaaaggtaa
     9301 tagaatagac atttttatga acagtgaagg aaaagcttca aattggggaa ggaaaagtat
     9361 agatatatat ctacattaaa ttagtgaata gaataacttt acttgaagga caaaaaagta
     9421 taaaatatga attaactaaa ctgttagatt attcttaaaa cataaaaata tatctaaggg
     9481 gggataagat gaaaatagtc tgcttaggag acagtttaac gtatggattt ggagtatcac
     9541 gaagtaattc atggacaaac attgtaaata aagagactcg attagaaata gtaaataagg
     9601 gtataaatgg tgatacaaca agtggtatgt tagctaggtt taatgaagat gttgtaaaaa
     9661 attctcctga tatagttttt ataatgggtg gcaccaatga ttttattgct ggtgcgggaa
     9721 atgaaattat taattctaat ataatggcta tggtgcatca gtcatattca aaaaatatta
     9781 tacctataat aggtatacca ttaaaaccag atattccaaa tgttagggaa gattggagtt
     9841 gttttactga ttttaatata gtatctcaaa acttagaatc atatagttat tggataaaaa
     9901 aattttgtat gacatttaat actaatttcg tagattttta ttcagaatat aataaaaata
     9961 tggaactaga aggatataaa aaattatatt ttgatgggtt acatccaacc aaagagggtc
    10021 atagaataat ggcagacatt tttattaatt caattaataa gtatatagaa gagttataga
    10081 ttacattaaa atgcttgttt atgcgtcagt tgcaataaat acatacagta gagaggggtt
    10141 tattatggat tatactaaaa cttatgaaga gtggataaag ggttcatatt ttgatgaaga
    10201 tacaaagcta gagttggaga atataaaaaa taatgaaaaa gaaatagagg atagattcta
    10261 taaggattta gaatttggga ctgcgggtct tagaggaata attgaagcag gaacgaatag
    10321 aataaataag tacacagtta gaagagctac ttttggattg gcaaactata tattagaaaa
    10381 cacaacaaaa gaagaaacaa gtagaggagt agtaatagct catgataata gacacaagtc
    10441 tagacaattc tgtatagaat ctgcaaatac tttggcagca tgtggtataa aagcatatat
    10501 atttgatagt ttgagaacta ctccagaatt atcttttgct gttagaagct taaatgcaat
    10561 cgctggtata gtcataacag caagccataa tcctccagaa tataatggat ataaggtata
    10621 ctgggaagat ggagcacaag ttatgccaga gattgcaaat gcaataacag agaaagtaaa
    10681 tagtatacat gattatagta caataccaac cttaacggaa gctaataaaa atttggtagt
    10741 tttgttggac gaatcacaag atactaaatt tatagaagca gtaaaaagcc aaattataag
    10801 aaaagattta gttaaaaatg taggtaaaag ctttaagata gtttatacac ctctttgtgg
    10861 aacagggaat gttccaataa gaagagcatt gaaagaagtt ggttttgaaa atataattgt
    10921 agttccagaa gaagaaaatc cagacccaaa ttttgcaggg cttgattatc caaatccaga
    10981 agagaaaaaa gctttaaata gaggtatatt actcgcaaaa gaaaaaggag cagaccttgt
    11041 aatagcaact gacccagatt gtgatagagt aggtgtagca gtaaaaacta ctacaggtga
    11101 gtatgcttta cttacaggaa atcaaattgg tggtatgctg acacattata taatagaagg
    11161 attaaaagaa aataacaaat taaaagaaaa tcctacaata ataaaaacca tagttacatc
    11221 agaatttgga gcagatatag ctaaagcaaa taatgttgat gtattaaatg ttcttacagg
    11281 ttttaaattt attggtgaaa aaataaaatt atttgaacaa aataaaaata gaagttatgt
    11341 atttggatat gaagaaagtt atggttactt agttgggact catgctagag ataaagatgg
    11401 tgtagtttct tcattgttaa tatctgaaat ggcagcattt tactattcaa aaggtatgag
    11461 tttatatgat ggtttaatag aactttataa aaagtatgga tttttcaaag aacagactat
    11521 atctttgact ttaaaaggta tagaaggtgt agaaaagata aaagaaataa tatcttattt
    11581 tagagaaaat caaattgatt atataaatag tattaaagta gttgataaaa aagattataa
    11641 aaatggtata gataatcttc ctaaatctga tgttttaaaa tactttttgg aagatgaatc
    11701 ttgggtagct ataagaccat ctggaacaga accaaaactt aaattttaca tagctgtaaa
    11761 aggagctagt gatattgaag cagataaaaa gattcaaggt ttgaaaaagt atatagatga
    11821 catggtcgaa aaattaaaat aaaatattat tattttataa gataattttg taataatatt
    11881 tatttagctg aaaattataa tttactttca ttgagtagaa tatggaaatg ttagtagaat
    11941 tgttattttc agcttttatt tgtgttattt ttacttaaat aattatatta tagaatgaat
    12001 ctgagtataa ttaatattgt ataatgaaat taatttgata gaaaagggga tatttatgtt
    12061 tggtatagat aagaaattga ttgaatatat aattgcaaaa tccaattaca gtgaattaag
    12121 ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc atatttcctt ttttaatatt
    12181 tacaataagt tctatagctt atataccgat tcttcattta aataagtata tagctttgtt
    12241 tagaaatatg atgccagaag gtgcttttgc tgtgttatct tcaatcatag tttctgcaat
    12301 agacaataga aacctgaaat ttttagcagt cagttttgta cttactatgt ggacattttc
    12361 aagagcagta aaagctctta ttaaaggaat gaatagagcc tataaagtta aagaaactag
    12421 gtcttttttt aaaattttat ctatatcatt tttatttaca ataatgcttt tggtccttat
    12481 ttttttatct atgatatttt tagtttacgg ggaaaaaata ggttatttta tttttaatct
    12541 tgtaggattg gatgagatat ttataaaaat atgggatatt ctaagataca ctgtaggtat
    12601 aataacaata atagtgatat ttacactttt atataaatac actccaaata aaaagttaac
    12661 tattaaagaa tctgcgccag gtgctatttt tgctactttt gcttggtttt tagtatcatt
    12721 tttatattct tattatacaa attactatgc taactatgaa gtaatctatg gaagtatagc
    12781 tgaaataatt gtacttatga cctggatgta ttttagtagt tggtctatcg ttattggcta
    12841 tgaggtaaat tcaagattgt attttagaaa gataagacat gaaatgttaa agtaaagtat
    12901 ttaaaataaa agagagtatt tagtattata aatgtaattg aattaataaa gaaatattga
    12961 catttaagtt aaaaagtcta gaatttttct gggctttttt tatttttagt ataacttgac
    13021 aaattttatt taatgggata gttttaattt ataatcatct ttaaaaagat gactattgat
    13081 aagtaagaga taaaattggt aagtatcaaa aaatatacta aataaaaatt atagtcctaa
    13141 tgagttaatt tctgtatttt caaataatgc agtatttagt catgtgtttt tatatgtgcc
    13201 tgtatttcta attaccaata tattagaata agctactgta attagattta gaaaaaagcg
    13261 acataaataa ttataaacaa ataataaatt acataaaatt aaatttattc acaagtatat
    13321 cttataaaat atgttataat taatagaaaa ttgtcatata attaattaag tagtattatt
    13381 ataaataaca ttgaaaggta gtggttagaa tatggatacc cataataaat atgtaaattt
    13441 tataaaaaat atacctgtac cttttttata ttgtagaatt gtaaagagac aagaagatat
    13501 agaatatcga gtagaatata taagtaaggg tatgggcaaa gtattacaat tagaagaagg
    13561 tatttgtgat aaaaatatac tagatgtatt gcctgtattt aagtctaaaa aatatttcaa
    13621 agagttgttc tcaaatgaag tggattgtat aaaaaggtac attccgacac ttaaaaactg
    13681 gataaatata aaaaaacaga taatagggga ttcatacata attttgtatt ttggtaaaat
    13741 tgtatttgat tatagacaga taatcgattc ctttgataaa aaggaaaaag tagcttatat
    13801 taaagacgaa gaaggcatat atatagattg cagtgaaaat ttaataccaa tattaaataa
    13861 caacattaaa acaacgaaag atatctttgg taaaaatgat atagaagtat ggggagagaa
    13921 tacaggaaaa ttatttagag atgattatag agaaggagta tctagtaaaa aaagattttt
    13981 acaaaatctt tttgaatatg aagaaacatt ttttatggtt gaaaaatatt ttttgtatga
    14041 tgaagacgaa cttttaggta ctatagggat agtagacaat ataatatata gtggttacag
    14101 taatagaaat tataactcaa aagatttaat gaagatgata gaacactcaa ttccagagaa
    14161 tatgttttat aaagatgtgt atggaaatta tattggattt aattcaggtt ttttgaattt
    14221 agcttgtatg aataaagaag aattattagg gaagaatagt tataaaatat cagaagaaga
    14281 agctttaata gataagattt ttgaaagtga taagggtgta gttgaaaata aaaaagtagt
    14341 tacgtttgag ttaaatataa gtatgaatga tgaaaacaag tgtattgaga ttacaaaaag
    14401 gccgtttttt gatagctatg gaagtgttat tggaataatt ggaacagcaa gggatataag
    14461 tagaagaaaa agattagaag aggaaatgga taaaactagg atggaatttt ttgcaaattt
    14521 atcccatgaa cttagaacgc ctataaattt aatatcatct tcattacaag tgatagaaaa
    14581 aaaagaagca gatttaatag aatcaaatga tactttaaaa agaaatctag gtataataaa
    14641 acaaaatgga aatagaatat tgaggttagt aaataatgtt atagacttta caaaaatgca
    14701 atcaggatat ttagatttta aaccagaaga atctgatata attgcattta tagaggagat
    14761 atgtatgtct gtagctgatt ttgcaagcca aaataatata caacttacat ttgatacaga
    14821 aatagaagaa ttttcaatgt tatttgattc agaaaaattg gaaagaataa tattaaattt
    14881 actttctaat ggaattaagt acaataagaa agatggaaaa ataaatatat ttttatatgt
    14941 gaaagataat gtatttaata tgaaaatatc tgatagtggt ataggtatac caaaagaaaa
    15001 aatagataaa atatttaata ggtttgaaca gattgacaat gaactttctt acagagttaa
    15061 aggaagtggt attggacttt cattagtgaa atctttggtt gagttgcatg agggaagtat
    15121 atctttaaaa agtcaacttg gtattggaag tgaatttata gtttcattgc ctgttagaag
    15181 taaaaataat attgaaaaat ataatcataa aagagaaatc agcaatgaac taagtaaaaa
    15241 attagaaata gagttttcgg atttataagt ttaaatataa aaaataaaat tttaaaaaag
    15301 tattgacaat tgatttagaa gggtttaaaa taaattacaa cataataaat aaaaaggcga
    15361 tgaataggaa gagtaaatct aattcgatta ccagagagaa gagtccgttg gctggaagac
    15421 tttttaaatt agagggattg aaaactacct aggagctact ttctgaagtc atctgcatta
    15481 gtagatttta gtaagaatag tcgttagctg cgttaaagtg taaagtgagt tttcatgaca
    15541 tatgaaaact aaattaggtg gaaccgcggg aataatactc tcgtccttat gttataagga
    15601 tgagagtttt ttgttattta aaaatataaa cagtaatcat aataattata gaaaatataa
    15661 aagcgatgaa taggaagagt aaatttaatt cgactatcag agaggaaagt tcacaggctg
    15721 aaagactttc taagtttgag aaattgaaaa ctacctagga gctgtggtct gaagttactt
    15781 taaaaagttt tagtaagact aaccgttaac tgcgttaaag tgtaaagtgg attttatcaa
    15841 taaatgataa aatcaaattg ggtggaaccg cgagtataga ctcgtccctt tatataggga
    15901 tgagtctatt tttattgcaa taaaagattg aaaataaaaa aaaatcggaa aattataaat
    15961 ttaggaggaa aataaaatgg agaataaagt gaaaattgga gtactaggat atggagttgt
    16021 aggaagtgga cttatagata taatagataa taataaagaa aaaagaaata tagaaatagt
    16081 tggaatttta gttaataatt tggaaaaaca taaggacaaa aaatattcta atataataac
    16141 taataatata gatgatatat ttaataaaga tatagatata cttgttgaag tcatgggtgg
    16201 tttagaacca tcattatctt atataaaaaa agcattaaac aataaaattc atgttgttac
    16261 agcaaataaa gatttgttag ctgaatgtgg agatgagctt gcaaaattag caagtgaaaa
    16321 taaagtaagt attaaatttg aagcttcagt tgcaggaggt ataccagtat taaaaccaat
    16381 aatagaatca ctagaaggaa ataatataga tagtataaat gcaattttaa atggaactac
    16441 taattttata ttatctaaaa tgtatgatga gaatctatct tatgatatgg cattaagaca
    16501 agcacaagaa ttaggttttg ctgaagcaaa tccagagtca gatgtattag ggtatgatgc
    16561 tgctagaaaa ctatcaatat tatcaactct agcttatgac aatagagtat attggaaaga
    16621 tgtgtattta gaaggaatta cagatataga tgaaaaagat attgaatatg caaaaaaatt
    16681 aaattgtaaa ataaaattga ttggtcaaag taagtatgaa aatgataaag taagtgcttt
    16741 tgttagacct gttttagtag aaaaagataa tatccttgct agaatagaca atgagttcaa
    16801 tgctgtcata gtgaatggag attcagtagg agaagtttct tttgtaggta aaggagctgg
    16861 aagtttagca acaggaagtg cggtttattc agatgttata gatataatag ataatagagt
    16921 ttcaagtata gattctttta ctaaagataa aatacaagtt aataaaatag ttagagaaaa
    16981 gtgtggagca cttcttagat ttaaaaaatg taataaagat gaaattctaa atatagttga
    17041 gaattgttta gtaaaattcg atattttaaa tgatgatgat gaacttgcta ttatggttta
    17101 tgctgattct gaatatgaaa taaacaacag tttatgtcta ataaaagata aaggatactg
    17161 cgaaaaaatg aataaaatgt tgaaaataag ttaaaaaaga aaaaatataa gtttatatgt
    17221 aacatatttt gacgatttta atactatata gtatgagatg aaacaaaaca tctcataaaa
    17281 aaattaaaat taaagggaga gatatgttat atggaaaata aaaaatgtta ttcagaagat
    17341 tggtatgaaa gaggagaatc tacagctaaa tggttccaaa atgatagaga agaatatgaa
    17401 agagaagcat atgatgaaga tagagaaaga agaggttcaa actgtggatg ttcagattca
    17461 ggagaaaata gacctagaaa ctgtgaaaga tttagaagag aagctgagat aagagaaaga
    17521 gaagcaagag aagcattttg tgaatcttca gagaaaaaga aagaggcatt agcatatgaa
    17581 tgtgaagcta gaaaattatg ggaagaagca gaaaaatact gggatgaata ttcaaaatac
    17641 aactataaag gaatcgaata tttagcagaa gctgctagat tatttgatga aggtatggaa
    17701 tgtgaagcta gaagaaatgg aaataatgga ggaaacaata ataattgttg ccataaatgc
    17761 cataaatgta attgtaactg ctgtagaaaa taaaaaaatt aattgacaaa tcaatagtta
    17821 aaatatatca tattatgtat aataaagaga aaattcagaa aaaaataatg attagaagaa
    17881 atataagtat atttattaga caaatagtgc tcagttagtg aggtgagtac aatatgatta
    17941 aactcccatc ctctagcttt tgatagaggg tgggagtttt tatttattta tatatcttaa
    18001 atgaggataa tattgtaaaa taaatgagta tgagattgta aaattaggag gtaaaaatga
    18061 aaagtattaa aacatcaagt atgaaagaaa aggtagatga aaagatatta agagagcaag
    18121 taagttctat aatagaagat ataagaaata ataaggatgt tgcacttaaa aaatataatg
    18181 agaagtttga tagaaataca agatatgaat ttagaattac taaggaagaa ataaaggaag
    18241 cttataagca tgtagatgac gaatttataa ataacttaaa gattgctgcc aaaaatataa
    18301 gagaatttgc taaagctcaa aaactaagtt ttgaaaaccc ttttgaaaaa gaaatttatc
    18361 caggtgtaat cttaggtcaa actaatattc caatagagtc atgtcttgca tatgttccag
    18421 gaggtcagta tccattgttt tcaacagctc ttatgcttat aatacctgca aaagtagcag
    18481 gagtaaaaag aatagtggca tgttcaccaa ctatgaaaaa tactgaaaag ataaatccta
    18541 agacattggt agctatggat atagcaggtg ctgatgaaat atatgcaact ggaggagttc
    18601 aagctatagc agcatttaca tatggaacag aaaagattaa tccagttgac ataattgtag
    18661 gaccaggaaa taagtttgta actgaagcta aaagacaatg ttatggacaa gtagggattg
    18721 attttgtcgc tgggccaagt gaagttctta taatagcgga tgaaacttca aatccagttt
    18781 atattgcagc tgatttatta gcacaatgtg agcatgattt aaatgcaaga ggaatattat
    18841 tgacaaatag cttggaagta gcacaaaaag ttgaaaaaaa tatagaaagt atgctaaaag
    18901 atttgccaac aaaggacata gcatatagtt catgggaaaa taatggagag ataatattgg
    18961 ttgatgatat ggaagaagcc attaaaataa gtaattttta tgcaccagaa catttagaag
    19021 ttgcagtaaa tgagtataat gatatttgtg atagattaac taactatggt tctttattta
    19081 ttggaaatct ttctgctgag gtgtttggtg actatgtttc aggtaccaat catacattac
    19141 caacacttaa agcatcaaga tatactggtg gtgtttgggt tggaactttt atcaaaactt
    19201 gtacaaagca gatatttaat gaaaaagcta tacaatcatt agcacctgta gctgaaaagc
    19261 tggcaaaaga agagggatta tatgcacatg caaaggctgc tgaggttagg tttaaaaagt
    19321 agtaaaaaat tttattttgg cagtcgagac ttagtattag actgagataa ggaaaacgct
    19381 gagaacctga ataaatacta tattttgtgg gtctcggcgc ttcttttata tctgtatata
    19441 atagaataat actatctgtg tgtttaaata ctaacttaat atttcttttc taaataatgt
    19501 ttatgaaata gttaatattt ttagttaata cttgaaaaaa acttttgcta actgtgcaaa
    19561 ttcatctgca tttgtcaata tagcagggtg tcctccctga ctaaaaatgt aaatacttgc
    19621 ttttgaaata aaagttgcca tttgcttgta ctcttcttca agattatgac gacacatttc
    19681 gtcttcctta cttcctgtaa ataaaattgg catttctaat ttacacaatt ctttatgaaa
    19741 caaagggcgt ttctcgtttg cacattgcaa tagagctttt gtatcaagat taacaacatt
    19801 ctcccaatct tttccatgac accattcata gaattttcgt gcttgaatat cttgctttga
    19861 tttttccctt ccagtgatta gattatctat gaagttatca ttaagagtac gtccatcaaa
    19921 gctatctgct ataattgctt caactaaatc gggacgttct aatccagtat taacagctgc
    19981 ccatgctcct ccacttgttc caattaagcc aactttacca caattcaaat gttcaatcag
    20041 tgctattgtc tgtaaggctt catcatgcca tatgtcagga gaaaattgag aaactctatc
    20101 cgactgtcca ttacctaaaa aatcaattaa gatacatcta aaattttctg catataatgg
    20161 cacaagtgct ttaaacatat tagaagaacc agtattacca tgcaggaaaa ttatcggttt
    20221 accttgtcca tattctttat aaaaacaaga attaccttga tactgaaaat atgacatata
    20281 ttttcctcct ttttatttgt taagttaatt ataacaatat aggtatgaat gaaaaacctc
    20341 atgttctttc ttagaataaa aatgtgccac gcataaaacg tgacacattt ctaattataa
    20401 attttgctgt ttaagtattc tttgtatgct tttctctgtc aaaaagtatt tctctgataa
    20461 ctgcttgata ctcattccat tttggaaatt tatacagatt tgagaattac gagaagctaa
    20521 gacttgtttg gtgtttgtgt tttcacccca atgctttttg tgctcttgct ttttgggaat
    20581 ataaatcact ttaccatcta tatattcttg cacttgctca atcagttctt taggcagtaa
    20641 gtcaatcgaa ttttgatagc tcattttgca cctcctaaat aattattctt taggattagc
    20701 aaaggctact gccactcaca attaaaaata aaatttttta ttatgtacaa tagcctttgc
    20761 tatgcacaag ttccaattcc cataaactaa atactccttt ctggtttgaa aaattcatct
    20821 gaacattgat ttcgctttta agatagagtt caatgacaaa tttacatttt tattatatat
    20881 attcagatag ataattgcaa ctgtctttgt atgaggatat tttattatag tcaatactat
    20941 atataatatg tatttatatc atcaaactaa tatgaacagt tgaaaggaat atgaacaata
    21001 aaacttagtc aggaagaata attatgaagc agacaataga gaaatttgat tttaaagttt
    21061 tattgaaaat ttattgacaa atttaacttg taaatattat actaaactta ataaataaag
    21121 tttataaagt aaggtgttgt ctatgaaaaa tatagttgga aaaccgcaaa cgataaaaaa
    21181 agtaaatgaa gatttaataa aaaacattat aaaaaatgaa ggtcctataa caaaaccaga
    21241 aatttcaagc attactgaat tgagtttagc tacagttaat aagattgtag agcaattagc
    21301 tttgaaaaat gaagttaaag taagtggact aagtgaatct actggaggaa gaagagctca
    21361 attatttgaa ataaatgcaa acttagaaca cataatagct ttatattatt atagaaattg
    21421 ttgtataggg gtagttgcca atttattagg tgaaataatt tatcaagaag aatttaatat
    21481 aagaatggat acgtatgaaa acgttaacca agatactttt tttgttatag atactctaat
    21541 aaaaaaggaa aaaggaaaca atataagggc tattggaatt ggtgtcccag gagttgtaaa
    21601 aaaaggtgta gttaaaaata taccaactat aaaaagttgg gaaggtataa atttagaaaa
    21661 tttaataaag agtaaataca atactaaagt gtttatagaa aatgatacaa atattactac
    21721 tattgggatt tatcaaaaac agtataaaga caaatataaa aatatggctc ttatgtattt
    21781 agaagatggt attggttcag gtatagttat aaatggtgaa ttgtatttag gttctacaaa
    21841 ttttgctgga gaattaagtt atctaaatat agaatttggt aagtataact ctaacaactt
    21901 aaatcttgaa gattatatta tgagtttaag aaaaagatat ttagaaagta aagataataa
    21961 atttaagaaa gaaattttat ctataatatc aaactttata aggactcttg tatgtgtcct
    22021 aaatccacaa gctatgatta tacagtgtag tatattaacc aagagtgaca tggagtttat
    22081 agaaaaagat gtaagcaata atgttggaga agaaaatttt cctaaattaa taaaagtaga
    22141 ctcattgaga gaaggaagta taaatggagt aattagtatg tgtttaaaag aaactgacta
    22201 tcaatattcc ttatctaata aaaaaggagg atattaatgt gaaatatttg gtttcaatag
    22261 atggaggggg aacaaaaact aaattttgtg taagtgactt agatggaaac atacttaaag
    22321 aacataccac tggttcaact aattataagt cagttggaat taaaaaaaca tatgaaaata
    22381 ttaataatgg atttaaaaaa atattaaaag atttatatat cgattatgac gatattgagt
    22441 acactgtatt tggtatatct ggttgtgatt ctcctaatga ttataagatt ataatggatg
    22501 aaatattaaa aattgggatt aacaaagaaa aaatatatct ggcaaatgat gcagttttag
    22561 ctttttatgc acaagctgat tcccctggct tagtaattgt ggctgggaca ggttcaatta
    22621 tactgggaat taaagaagat ggagaaatat atagagttgg tggatggggg tataatttta
    22681 gtgatttggg ttctggatat gatataggga gaaaattatt aaagaaagta ttgctttatt
    22741 gtgatgaatg tcatgagtat agtgatttat ttagttgtgt cttggacttc tttggagcaa
    22801 atagttttga acaacttgca tatatgatta ctgatataaa tgataatgtt gaaatagcaa
    22861 acttagcttc attagtgatt gattgtgccg aacaaggtga taaattggct attgaaatat
    22921 taagagaatc atcaactgaa ctatctaaat tagctcaagt aatattgaga aaaatatcta
    22981 atccaatgaa aatggataaa agcgaaataa atatagtttt gtctggtggt acattaaata
    23041 aaactgtata tgctcaaatg ttaatgaata atttaaaaac agaaaataca gataaaaatt
    23101 taaaatttat atctcaagaa aatcagccag tatatggggg gataagactt gctatggcat
    23161 tagctaatag gagggtcaag catggataga aaaaaggtaa tagtacaaat ttttactgga
    23221 ggttttaagg ctaaagaaat aaagtttaat gatataaaat gtaaactact agaattattg
    23281 gactgtattg atatagaaaa ggttatcatg ggatggagtg tagatgaaga attatatgta
    23341 gaaactaaaa aactattaaa taaatataat gttgaactat atctttggtt gccatgtttt
    23401 tctgaaatag gattacttga aaaaagtaat ttactaattg attatatggg gaaggaagtc
    23461 aagggctatg ctttacaaga ggatgaaaat tttgagtttt attgcccaaa taataaggaa
    23521 aatataaaaa atataaagaa aatatatagt acttatttta gtgatattaa atttgatgga
    23581 atttttttag ataaaataag gtatggttct ttttcgaata aactaaatgg tgtttttaat
    23641 tgtttttgta aagattgttt aaatatatat ataaaaaata atattgataa agagtcgtta
    23701 atgagagaaa tgaataatgt gtctaaggga gttatagata atgaagatac tccatttggt
    23761 ataacagaat acattaatgg aaaatacaaa tttaaaaata aaatatggga agattttttt
    23821 gaaacaaaaa atcaaaatat atacaaggca ttattagaga taagtagtta ttttaaaagt
    23881 aagggattga aaataggtat agatgctttt tctcctttta tttcttactt tacaggtcaa
    23941 gatttagaaa aattacaaga agtagcagat tttataaaac caatgatgta tagaactaca
    24001 aaagctccag ctggtttacc atttgaattt gaaaggttta ttgatgaatc catacataag
    24061 aataaagaga ttggaaaaag aaatttctta gatattttag gaattaagga gtatgaagga
    24121 gatgtatatc ctatagaatt tgtactagag gaaatcgatt ttatgacaag aaatcttaat
    24181 aaagatatat atgtaggagt tgaaataaat aaaaaggaaa ttatatctcc tgttacacct
    24241 aagtacataa gtgaaaatat ttcaaatata aataagacaa cagtaaaagg atatgtttta
    24301 tcttgggatt tgttgtcagc accaaaagaa aatattgatg aaattataaa atggtttcgg
    24361 aattagtgag gtgataaagt gagtaagata ttggaaatgc aacaaatata taaaagtttt
    24421 ggaaaggtag atgttttaaa agggataaat tttgagttag aaaagggaga agtaagagct
    24481 cttttgggag ctaatggagc aggaaaatct acacttatta aaattttagg aggagttcat
    24541 tctcaaacta aaggtaaagt aattttgact ggtaatgaaa taatgtttaa aaatccacat
    24601 cattctaaag aaaatggtat tagtataatt tatcaagaat taagcttgat accaacgctt
    24661 acagtacttg aaaatatttt tcttggtaga gaaattttaa aaggaagtgt gtttttaaac
    24721 aagaaagaca tgcttgagga atatatgaaa atttgtgagg attttgattt taatatagac
    24781 ccacatatag tagtttctaa tttaagtgta gcaaaccaac aaatggttga gataatgaaa
    24841 gctgtttcat gtaattctga aattatcata atggatgaac ctacaacatc acttactaat
    24901 aatgaaaaag atgggctatt taaaataata agtagactaa agtctaaagg taaaacaata
    24961 gtctatattt ctcacatatt agaggaagtt tttaaagtat gtgatagtgc aagtataatg
    25021 aaaaatggaa ttatgattgg aacttatgat gtagaggatt taactaaatt aaagataact
    25081 caacttatga caggaaatga taatcacaaa cttattgaga agaaggatta tacatttgca
    25141 aaatatgaag agccaccagt actagaagta aagaatataa gctggtctaa caaaataaaa
    25201 aatgtttcat ttaaagttta tagaggcgaa gttgtaggtc ttgcaggtct tgtaggttca
    25261 agaagaactg aaattataaa tttgatattt ggtatagata ataaagatag tggagatatt
    25321 tttatcaatg gtaaaaaagt agacataact tctcctaagg ttgctataaa aaacaaaata
    25381 ggattgattc ctgaagatag aaaaaatcta ggattaattt tagaacaagc tatatacaca
    25441 aactcatcat cagtacaaat tgataaattc aagaaatttt tgtttataga tagaaataaa
    25501 gaaattgagt tttctaaaaa aggtgttaag gaattaggaa taaaagttag tgatgtaaag
    25561 caagaagtaa aagagcttag tggtggaaat caacaaaaag tagtagtttc taagtggtta
    25621 gagaaagatt tagatttaat aatatatgat gagccgacaa agggaataga tattgctgca
    25681 aaagaagata ttttcaatac aatcaaagaa ttttcaagta aaggaatagg agtaatattt
    25741 atatcttctg atttagaaga agtaataaga gtaagtgata gaattttagt aactagagaa
    25801 ggttctattg tagatgattt aaaaaatgag aatatacagg ttcaagatat aatgaacagt
    25861 atatttaatg tataaaaaat gaaaatggag gactgagatg aagaaagctt ttgagaagtt
    25921 aaatttaaga gattatggag tattattagg ttttttagct ttatgtatag ctataagtat
    25981 tgcaactcca tcatttttag gtaaacaaaa tatacttaat cttttacgac aatcttctat
    26041 aattggaata atttcagcag gtatgacttt tgttataata tctggaaact ttgatatatc
    26101 agttggagca gtagcagctc ttgcaggtgc tataacaatg aaatttgcga ctggtggaac
    26161 taatttgttt gcatccatgt tcttaggtgt agcgatttgt gcagtaatag gattaatcaa
    26221 tggtatagta gttgcaaaga taaatgtacc atcattaata gccactatgg ctatggtcac
    26281 tattgtaaga ggattgctgt tgatgcttac aggaggatat ccaattactg aaaatatacc
    26341 aatacttgat tacattggaa atggatattt gtttaatata ccaataccag tagtaatttt
    26401 ctttatagtg gtgttagctt cgtttattgt acttaataaa actaagtttg gtagatatgt
    26461 ttattctgta ggagggaatc aagaagcaag taaacttaat ggtataaatg tagattctca
    26521 caaagtgaaa gtgtttatta ttaatgctgt acttgcagga atagctggaa tcgtactaac
    26581 aggaagactt gggactgcaa ctgcgattgc aggtgaagga tatgatatgg atgcaattgc
    26641 atctgtagta ataggaggta catcagtggc aggaggttct ggttctgtac ttaaaactgt
    26701 tataggagta ttacttatga gtgttataaa caacagtttt aatcttcttg gaatagatgt
    26761 atatttccag tacatattta aaggattaat aattttagca gcagttggat ttgattctta
    26821 tagcaagaaa aaacttgctt caagataaaa aataaggggg atttttttat gaaaaaatta
    26881 atgaaacatt tagcattgtt gctatcactt gttatgatat ttggtttagt tggttgttca
    26941 aatggtggtg acaaagaaaa aaatggagaa aagaaaatag cagtattact accgggttca
    27001 acaggttact ttgtagcaac taaacaaggt atagatgcaa aatctaaaga attaggtgta
    27061 agcgtggaat atgcagatgc tcaatgggat gctagtaaac aattatcaca agctgaagat
    27121 tttatggcta aaggggtaga catgattgca ttatgtggag ttgattctgc tgtaagtgag
    27181 agaatagtta aagctgctaa tgattcagac gttcctatag tagcatttac aaattctata
    27241 ggaagtaacc caactggaga atttaaaggt ttagttacat atataggaca aaatgaggaa
    27301 gaaacaggag cattaactgg aaaaattgct aaaaacttat taggagaaac tggtgggaaa
    27361 gctgtattaa tagaaggagt accaggaact actccacaag tattaagaaa aaaaggtctt
    27421 gaaaaagaat taaaagatag taatatagaa atagtttata atcaaacttc aaattgggag
    27481 aaagagcaag ctttaaaggt aacagaagat ttaattcaaa agaaaactga ttttaatgtt
    27541 ataatatgtc aagatgataa ttctgctaca ggtgcaggtc aagctttaaa agatgctggt
    27601 cttaaggata aagttaaagt cataggtttg ggtggaagta aagatggatt aaaagctata
    27661 actgatgggt taatagatgg tacaacttat atgtctgctg tagaagaagg tggattagtc
    27721 atagaaaagg catctaaatt ccttaaaggt gaaaaaatag aacctgttac acaaattaaa
    27781 caagttggag ttaacaaaga taatatttca gagttcaagg gtgaatggta aattgtcata
    27841 gataataaat aagattttta gagttacttt agaaactata taaagtaact ctttttttaa
    27901 tttaatttag tgataaatta ttgaatagta tttctaaaca aataaaaaat taatataaac
    27961 aaagacttaa ttatactaaa ttataagtaa aactaatata aacttatgga aaaaaggaaa
    28021 acgtctaaag tattggaatt actatagtgt aggtgtggta ttaaaaaaac ataaaaaaac
    28081 ataaaaaaat tgttgaaaaa aatatagaaa ttttgtataa ttaaagattt gaaaagtgcg
    28141 gtatacaaag ccaattcttt tgtatataat atatataaat tcagaaaaag gaagggatat
    28201 gtatggccag gcggttattt ttagaaagga ttggacttat atttttaggt tcagcaatat
    28261 tagcatttgg agtttataat ttttattatt taaacaacat aacagaaggt ggagtgttag
    28321 gtattttact actgttaaaa aatttattca atatacaacc agcaatagca aatgttgtaa
    28381 tagatagttt actactttta gtaggatata aatttttcgg taaaaaattc cttatatatt
    28441 caatagtagc ttctataact ttttcagtgt tatatgactt atttgaagct ataggacctc
    28501 tagtaccaca atcacaaaat atgcttttat caactatttt ggcaggtgta actgttggaa
    28561 ctggtgtagg tatagtagta aaagcaggtt gtgcatcagg aggagacgat gctttagcat
    28621 tagtaatttc taagactaca tctttgaaca tagggcaagt ttatttagca acagatgtta
    28681 ttgtattgtt gttatcatta ttttatctat cagcgtttga tatcttttat tctttgattg
    28741 ctgttacaat tagtggcaag gtaattgatt ttatttatta tcatggtaaa agtttggata
    28801 tggatataag taatgatata gttccagaat gttaatataa attaaataac tttagatttt
    28861 attaggggtg gcagatttgc cacctttttt ttgtctaaat ctttatagaa agtataatcc
    28921 catctaggtt tttgcaaatc tcaaatatag aattatgttg gaatgactgg gaatacgatt
    28981 gctagatgtg cattttaaaa acccaatttt atatttatga aatactaatc attaatggat
    29041 gtttcttaat cctatcttta tacctaaaat gataagataa atgaagtagc aatacaaagc
    29101 taaaatttgt aaaagagatt aaattaaatg gaggagctaa aatgttggat atagttatga
    29161 tagcattgtt attcatagga tttgtttcca tgaagttact tgctgattgg tgtgggaaac
    29221 aaactgaaaa aaaataatgt aatgtatgga gggattacct atgtggtttc tggcagttgt
    29281 aattataatg ttaataatat atttggtata tgcgcttatt aatcctgaaa aattttaaac
    29341 agtataaggc taataagtaa gttgaattgg ggggaacaat ttttatggtg cagatagtaa
    29401 tagtgttagc gatttttatg attttagtaa tacctatggg caagtattta tatcatatag
    29461 ctactaatca aaaaacattt ggagatagac tatttgataa agtggacaat tttatatata
    29521 aggtatgtag tatagataag aaaaaagaaa tgaactggaa acagtatgca ttagctttat
    29581 tatttacaaa tgcagttatg gtatttatag gatatatcat acttaggact caaagtatgc
    29641 atatttttaa tccaagtgga attaaaagta tggaacaagg actatcattt aatacaataa
    29701 taagttttat gacaaataca aatcttcaac attattcagg tgaatcagga ctttcatact
    29761 ttagtcagat gacagtgata atatatatga tgtttacatc agctgcaaca ggatatgcgg
    29821 ctgctatggc atttgtaaga ggtctagtcg gtaaaaagaa aacattggga aatttttatg
    29881 tagatttaat taggataaca acaagagtac ttttaccagg agctttaatt attgggctta
    29941 tattagttac acaaggagta ccacaaactt ttgctgggac agaaacagtg actactatag
    30001 aaggaaagct tcaagatata gctagaggtc ctgtggcagc tcttgaatca ataaaacatt
    30061 taggaacaaa tggtggtgga ttctttggtt caaattcatc acacccattt gaaaatccaa
    30121 caataatatc aaatatagta gaaatattat ctatgatgat attaccagga gcttgtgttg
    30181 ttgcattcgg tcacatgata aagaataaaa aacaaggttg ggttgtgttt ggcgcaatgt
    30241 caatcatatt tttaataggt ctagtagtgt gctttaaagc agaaagtgca ggaaatccta
    30301 ttttatcaca attagggctt aatcaaagta tgggaagtat ggaaggtaag gaagttagat
    30361 ttggtatagc acaatcatca ttattcacaa cagtgactac ttcctttacg acaggtacag
    30421 ttaataatat gcatgataca ctgactcctt taggaggatt agtaccactt ttaaatatga
    30481 tgctaaatgt agtatttggt ggtaaaggcg taggtctgat gaatatgttg atgtacgcta
    30541 taatagcagt attcttatgt ggattaatgg taggaagaac accagagttt ttaactaaga
    30601 aaatcgaagg aaaagaaatg aagcttattg ctttacttat tatattacat ccacttttaa
    30661 tactaatgtt ctctggtctt tcagtagcaa taccagcagg acttgaagga atttcaaatc
    30721 ctggattcca tggattatca caggtattgt atgagtttgc atcttcagca gccaataatg
    30781 gttcaggatt tgaaggtcta ggagataaca ctatgttttg gaatataaca acaggtatag
    30841 ttatgttctt tggaagatat gtgtctatta tagtactact agcaatatca agtttgttag
    30901 catctaaaaa agctgtaaat gaaagtatag gaacacttag aacggataat tttacattta
    30961 caatagtttt agtacttgtt gttttaattg ttggtgcttt aacatttttc ccagccttag
    31021 cgcttggacc tatttcggag catctagtgt tgtggcacta aaaaattggg agggagtaaa
    31081 gttttatgga aactaaaaaa acaaagttta ttacaaaaga tattttcaaa acttccatta
    31141 tagaagcttt taaaaaatta aatcctaaat atatgatgaa aaatccagtt atgtttgttg
    31201 tagaaattgg atttatagta acattgatac ttacaatagt acctagtttg tttggtgatg
    31261 aaggcaataa cttaagagct tataatggga ttgtaacaat aatcttattt ataacagtcc
    31321 tttttgcaaa ttttgctgaa gctgtagctg aaggaagagg taaagctcaa gcagatacac
    31381 ttaagaaaac tagaaaagat acagtagcaa gggttataga tacgttagga aatgaaaaaa
    31441 tgataagtgc aagtgagcta aaacttggtg atattgtttt agtaaatgct ggagaagtta
    31501 tcccaaatga tggagaagtt atagagggta tagcttctgt ggatgagtca gcaataactg
    31561 gagaatctgc acctgtcatg agagaatcag gtggagattt cgcatctgtt acaggtggta
    31621 ctacagttgt aagtgactgg ttaaagataa gaataacaac aaaaccagga gaatcgttct
    31681 tagataaaat gatagctctt gtagaaggtg cttcaagaca aaaaacgcca aatgagattg
    31741 ctttaaatac tttgttagta gggttaacac ttatatttat ggttgtttta atatctcttt
    31801 atccaatggc aaactatgca ggtgtaaaga ttccaatttc tactatgata gctcttttgg
    31861 tatgtttaat accaactact ataggtggat tgttgtcagc tattggtatt gcaggtatgg
    31921 atagagtaac tagatttaat gtaattgcaa tgtcaggaaa agcagtagaa gcttgtggag
    31981 acgttgatac tatgattttg gataagacag gtacaattac ttatgggaat agacttgcat
    32041 cagaatttat agcagttgga aatgcagatt caaaagattt aataaattat tctgttatgt
    32101 gttcattaaa agatgataca ccagaaggaa aatctatagt tgatttaggt atgaaattag
    32161 gttcaacagg taagacaaaa gaggcagagg aagctgagtt tgtagaattt tctgcacaaa
    32221 ctagaatgag tggtattaac ttagctgatg gtagagctat aagaaaaggt gcttatgata
    32281 gcataataaa aagagtaaaa gaatctggtg gtacagtgcc agaagattta gaagaaaatg
    32341 taaatcgagt agctaaacta ggagggacac ctttagtagt gtgtgtagat aatgagatat
    32401 atggaattat ctacttaaaa gatacagtaa aatcagggct tgtagaaaga tttgctagac
    32461 ttcgagagat aggaataaag acaataatgt gtacagggga taatcccttg acagcagcta
    32521 caattgcaag agaagctgga gtagatggat ttattgcaga atgtaaacca gaagataaaa
    32581 tagatgcaat aaaaaaagaa caattagagg gtaaaatagt tgcaatgaca ggtgatggaa
    32641 caaatgatgc tcctgcactt gctcaggcag atgtaggtct tgcaatgaat agtggtacaa
    32701 catctgcaaa agaagcagca aatatggtag atttagattc agacccaact aagatattgg
    32761 aagttgttga aataggaaaa cagcttttaa taactcgtgg ggctttgaca acatttagta
    32821 tagcaaatga tgttgcaaaa tattttgcga ttataccagc tatatttact cttgcaatac
    32881 ctcaaatgga gatattaaat gtaatgaaac tttctacatc atttagtgcg atattatcag
    32941 ctttaatatt taatgctata attatcccat gtttaatacc tcttgcaatg aagggagtaa
    33001 aatatagacc tatgaaatca caagctatgt taatgagaaa tatgcttata tttggtcttg
    33061 gtggagttat agtaccattt ttaggaataa aattaattga tttattgatt acacctttag
    33121 taatggttct taatttaggt taataaatgg aggatataaa aatgaaaaca ttaaagccag
    33181 cattacttgt aagtattgta ttgttagttg tttgtggact tgtatatcca cttgttttga
    33241 caggtgtaag ccaagttgca tttaaagata aagctaatgg aagtatgata gaagtaaatg
    33301 gtgtaaaagt aggttcagaa cttattggtc aaagctttac agatgcgaga ttctttaagg
    33361 gaagagtatc atcagtaaac tataatactt atacaaaaga agatttagta ccagataaag
    33421 atggaaatac atcttatgga ggtgtatcat cagggtcttt taattatgga gctacaaatc
    33481 cagaacttca tgatagagtg caaaaagata tagaaaagtt tttaaaggat aatccaactg
    33541 tcaaaagaga ggatatacca acagatttac ttacagcatc aggttcagga cttgacccta
    33601 atataagtcc agcctctgca aaaattcaga taccagctat agcaaaagcg tctggaatat
    33661 cagaaagtaa attacaaaaa attgtagatg ataatacttc caaaaaactt tttggagttt
    33721 taggtgaaga tagagttaat gtactaaaag ttaatgtaga ggtcgcaaaa atattaggtc
    33781 ttatatagta ttttgtggaa ttttagcaac aaaatattac gcaagattta atttaaaggt
    33841 taaattttaa agagtgaaaa tcgataatga ttttcacttt tttatttgaa tattactaat
    33901 atttggaata aagtgattgt aagtataaaa tccaaaaaaa ggttgcatta tcaaaatgat
    33961 aatactataa tatatttatc gttatcaatt tgataataga tttggacata taggaggaaa
    34021 tagtatgtta tataataacg cattagagtt gataggaaac acacctgtag taaggctgaa
    34081 taatttaggc tgtactaatg ggtgttctaa catatatgta aaattagaaa aagtaaatcc
    34141 agcaggaagt ataaaagata gagctgttta tgaaatgtta gagggcttag agcaaagagg
    34201 agaattaaaa aagggagatg tactagttga agcaactagt ggaaatactg gtattgcatt
    34261 atctatgata ggaaaattaa aaggatatga agtagtaatc gtaatgcccg aaacaatgag
    34321 cgttgagaga agagatttaa tgaaggctta tggagcaaat ttggtgctta cagatggtaa
    34381 agctggaatg gcaggttcca tagaaaaagc aaatgaactt ttaaaagaaa atccaaacta
    34441 taaaagctta aaacaatttg aaaataaaga taatccaaat gcacattata aaacaacagc
    34501 agttgaaatt atgaatgatg tcaaagattt agatatattc gtatgtggag ttggaacagg
    34561 tggtacttta ataggaactg caagatattt aaaagaacaa aatccaaata taagagtaat
    34621 tgcacttgaa ccaaaaaaat ctccagctat atcagaaaac aagactggac ctcataaaat
    34681 acaaggaata ggtgctaact ttgttcctga aaattatgat tctagtgtag ttgatgaagt
    34741 aatattagta gatgatgaag atgcatttga tacggttaaa ttacttgcag caaaagaggg
    34801 aattttagta gggatatctt ctggagcaaa tatatttggt gctatagaga tggcaaaaaa
    34861 gtatccagat aaaaagatag ttacagtagc tccagatgga gttgataagt atatgtctat
    34921 gggaatattt taataatttt atttgaggga gtgaatactt tgtttaagaa aataaataaa
    34981 gatattgaat atataatgaa gaatgatcca gcagctaggt ctaagataga agttttttta
    35041 ctttatccat cagttcatgc aatgataatg cataggatgg ctcatgctct ttataagaag
    35101 aagaagcttt ttacagcaag attaatatcc cagatatcca gatttatgac tggtatagaa
    35161 atacatcctg gtgctaaaat gggggaaggt atattgatag accatggaat gggtgttgta
    35221 attggagaga cagctgaagt aggtaatagg gtgacaatat atcaaggagc tactttagga
    35281 gctacaggaa aagatactgg aaagagacat ccaactgttg gtgatgatgt attaattggt
    35341 gctggaacaa aaatactagg acctttaaat ataggttcaa attcaaaaat tggtgcaaat
    35401 tctgttgtcg ttaaggatgt acctaatggt gcaacagttg ttggtatacc agcaaaaata
    35461 gtaaaaataa gaaacctaga acctgtaaaa aagaataaaa aagaagtgtc ctatgagtat
    35521 gatgaattag ataatgtata ttatatatag gatttatata actataaaat aaactttata
    35581 aaaattattt taaaaggtat ttttttcaaa tttgaggaca taataaaaaa gaactgaatg
    35641 ttacagtttt ggttcatcaa tctttgataa acaagatttt aaatttgtag cgtctaggag
    35701 gattttatta tgaaagcaaa tgttaatcaa gatacttgta taggttgtgg attatgtcca
    35761 tcaatatgtc cagaagtatt tgatatgaag gatgatggga aatcacatgt tatagttgat
    35821 gaagtaccaa gtgatgctga agaatcagca gcagaagcta gagaaagctg tccagttgat
    35881 gcaatagatg tgcattaata aaataaatgc ctataaaaat tgaattttca atttttatag
    35941 gcatttgtct tttattcagt tagtatttca acacctgttt ctgtaactaa aaccatacat
    36001 tcccattgag cagataattt accatctttt gtataaatag tccaattatt ttcttcatca
    36061 acaaaaattt cgtgagttcc ttcatttacc ataggttcta ttgtgaatat catcccagga
    36121 accaatagca tctcagtccc ttttttagat acataactta caaaagggtc ttcatgaaag
    36181 cttaatccaa ctccatgacc acctatttct ctaacaacag agtatccatt tttctttgca
    36241 taagagttga tagcatcacc tatatctcct aaaaatcccc atggtttggc aacttcaaga
    36301 cctttttgga cacattcctt tgtaacttga actaattttt ttgcattttc attaacatta
    36361 cctatcataa acattcttga agcatctgaa tagtatccat taaaaatagt agaaacatct
    36421 acatttataa tatctccatc ttttaagata atgtccttac ttggaatacc atgacaaact
    36481 tcattattta ctgatgtaca tacactttta ggaaatccat tatagtctag tggagcaggg
    36541 atagctccat tttgtagtgt aaagtcatga accaacttat ttatatcttc tgtagacatt
    36601 ccaatttcga ttttatctga aatataatcc aaaatttttg tgtttattac agcacttttt
    36661 tttatttcct caatttgact ttcatttttt ataatatcat gagttggtac aatatgacct
    36721 aacttagcaa atgaatctat tttattatcc atatccatat gacatttttt atatttttta
    36781 gtacttccac accaacagct atcatttcta tttaacttca taacttttca tccttctttt
    36841 attatttatt taaattatgg gttacataaa ctattttaaa acataaccat tataaatatt
    36901 ataacataaa tactaagaaa tcctttatag atagtgattt attcaaagta taaattatta
    36961 tatggttatt gttgatgaaa agaggatagt taaatgtatt attggatatt agcgcactta
    37021 atagtttagt tcaagttttt caagaaaatc atatgttaga ggacaatttt cctttaaagt
    37081 attttttgaa tcttcatcaa gataataatg tacaaatgat tctgcaaaaa attcttctat
    37141 aaacttgctt atatatgcat gtgcaacttg cttagggtca taaagcttat atctttcctg
    37201 agcaaatact tccataaatt cttcagaatt tgatttttta tttaaaacat ttttatctat
    37261 agcatgtgca gtttcatgta attctaaatt ttttgatgaa tgtacatttg catagctagg
    37321 attactataa cctattttag ccactataga attatttcta tatataccag gtacatcttt
    37381 ccaagtcttg cctgatctta cccaacatgg aggtaattga tattttaaat cactaaattc
    37441 tggttcatca gttagatttg aatttattaa ttttattttt atatcctttt ctttaagtgt
    37501 ctgtaaatat ttaatattta tttttgcaag tctgtttttc atattttcaa cttcatcttt
    37561 atcataattc aagtcatcag gaagtataag catttgttca attatatcta tactactgtc
    37621 ttttatattt tcagattctt catcaaattt atattgaaga gtattatttt catagatagt
    37681 tatatttgta ttattttcta tagaataaga gtgaatatcc tctaaatcta aaatattcat
    37741 ttgttgtaaa atatgttttg tttcgccact ccacactact aaagaaagtg aattaaaatt
    37801 aattagtgga ttttcttttg cttttatatc attgcttata tctacatcaa atacatattc
    37861 aattgagtct tctatgttaa gactatctgt attcttttta atatataaat acgcttgttt
    37921 taagtctata taaatctcat ctaaagattc gtcataaaat tctgataaat tattagtaaa
    37981 gagttcttca cttaaaagtt catttttatc agttatactt tcaattaaaa tattaatttc
    38041 ttttatcata tcgtaaatac ttatttttct atttgctttt tcactagata cattgcatgg
    38101 cgcaagagca atgcaataaa aatctttttc ttttttataa aaggctttaa gtgattttga
    38161 taaaagtgta aagtctacac ctatctgtaa gttgacctta agtgagtcat tttgtaataa
    38221 ttcagtatta tttcttttag ctgtatttaa attgttttcc ataatcattg cccccttttt
    38281 attagggtat cataattttt ttatatgtgt caatttgttt ttttgaaaat tataattttt
    38341 ttgaaaattt tattttttat aatttgtgaa ttttttatta tagaaataaa tatgttatta
    38401 ttatgtcaaa atttagcatt agattcatgt agagtatgtt ttatacatat attataatta
    38461 attctttgaa gattgcatat tcaaaataca caccattttt tttaattcta ta
//
LOCUS       DAELJO010000012        38187 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELJO010000012 DAELJO010000000
VERSION     DAELJO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.012"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            31..1143
                     /locus_tag="KPB88_000579"
     CDS             31..1143
                     /locus_tag="KPB88_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG0052887.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="KPB88_000580"
     CDS             1397..3421
                     /locus_tag="KPB88_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0052888.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="KPB88_000581"
     CDS             3641..4327
                     /locus_tag="KPB88_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG0052889.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="KPB88_000582"
     CDS             4422..5477
                     /locus_tag="KPB88_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG0052890.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="KPB88_000583"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="KPB88_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0052891.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="KPB88_000584"
     CDS             7194..8900
                     /locus_tag="KPB88_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0052892.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYVFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KPB88_000585"
     CDS             8922..9995
                     /locus_tag="KPB88_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG0052893.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KPB88_000586"
     CDS             10007..10729
                     /locus_tag="KPB88_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0052894.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KPB88_000587"
     CDS             10745..11902
                     /locus_tag="KPB88_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG0052895.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KPB88_000588"
     CDS             11905..12744
                     /locus_tag="KPB88_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0052896.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KPB88_000589"
     CDS             12759..13934
                     /locus_tag="KPB88_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG0052897.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KPB88_000590"
     CDS             13963..14712
                     /locus_tag="KPB88_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0052898.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KPB88_000591"
     CDS             14734..15642
                     /locus_tag="KPB88_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0052899.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KPB88_000592"
     CDS             15690..16442
                     /locus_tag="KPB88_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0052900.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="KPB88_000593"
     CDS             16396..17763
                     /locus_tag="KPB88_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG0052901.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="KPB88_000594"
     CDS             17769..18860
                     /locus_tag="KPB88_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0052902.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KPB88_000595"
     CDS             18944..20857
                     /locus_tag="KPB88_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0052903.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KPB88_000596"
     CDS             21056..21763
                     /locus_tag="KPB88_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0052904.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KPB88_000597"
     CDS             22065..24176
                     /locus_tag="KPB88_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG0052905.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KPB88_000598"
     CDS             complement(24374..25339)
                     /locus_tag="KPB88_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0052906.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KPB88_000599"
     CDS             complement(25624..26580)
                     /locus_tag="KPB88_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0052907.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KPB88_000600"
     CDS             26948..27736
                     /locus_tag="KPB88_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0052908.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="KPB88_000601"
     CDS             complement(27859..28329)
                     /locus_tag="KPB88_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG0052909.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="KPB88_000602"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="KPB88_000602"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG0052910.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="KPB88_000603"
     CDS             29155..30060
                     /locus_tag="KPB88_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0052911.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30375..31922
                     /locus_tag="KPB88_000604"
     CDS             30375..31922
                     /locus_tag="KPB88_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0052912.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31925..32323
                     /locus_tag="KPB88_000605"
     CDS             31925..32323
                     /locus_tag="KPB88_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0052913.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32363..34096
                     /locus_tag="KPB88_000606"
     CDS             32363..34096
                     /locus_tag="KPB88_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0052914.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34328..35539
                     /locus_tag="KPB88_000607"
     CDS             34328..35539
                     /locus_tag="KPB88_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0052915.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35732..35992
                     /locus_tag="KPB88_000608"
     CDS             35732..35992
                     /locus_tag="KPB88_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG0052916.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36346..38058
                     /gene="ptsP"
                     /locus_tag="KPB88_000609"
     CDS             36346..38058
                     /gene="ptsP"
                     /locus_tag="KPB88_000609"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG0052917.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT        15280 a         3991 c         6747 g        12169 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatgtt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttggg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggagggca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgtagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagatttg aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 acctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattaaaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatattgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ttttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagagg atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagatc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatcgcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaagaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttactcac aagacatatt gaggtagata
    30481 ttaaagtttt aattttaatt ttattaacct tgtttagtat aaatgtattt atttcatatc
    30541 ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat tcagataagt
    30601 ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat attatcagta
    30661 atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa cttataaatg
    30721 atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt aacttaaaaa
    30781 attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt gtattaataa
    30841 taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatt aagaataaaa
    30901 ttgtgatact aagtcagatt gacagtttta aaatggatat aaatattgag gcttctattg
    30961 gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat aatgctgata
    31021 tggcaaggct taaatcaaaa ggactaaagc acatagagta tgtagaattt gataatgcta
    31081 tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg gctattaaaa
    31141 ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt aacattatag
    31201 gttcagaggc attgataaga tggttgcacc catcattagg catggttgga ccaaataagt
    31261 ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg ctaattcaag
    31321 aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta ccagtctcaa
    31381 taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt aaactgatgt
    31441 ttaatacata taatattcca aaagaactta tagaattaga aattacagaa acgacagcac
    31501 taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta ggtatgaata
    31561 tatcattaga tgattttgga gtaggtaatt caaattttat caatcttaag gggataccac
    31621 tagatattat aaaaattgac aggtcactta tattggatat agttacaaat acaaaaacaa
    31681 agtttatggt gaaagcaatt gttaaccttt cacatgattt aaacgtaact gttatatgtg
    31741 aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt aatttcatac
    31801 aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt cttactgacg
    31861 gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt gaggaggagt
    31921 aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta tatttaataa
    31981 tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata taccaatact
    32041 aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt ttaacaaaga
    32101 agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa gaactattgt
    32161 agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat ctaattgcag
    32221 taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa aagaaattta
    32281 taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt tcagctatga
    32341 taataattgg aggttttcgg ttatggatga tatatctcaa gataattttc tactgtctaa
    32401 agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat attatactcc
    32461 taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata taattaaaaa
    32521 tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt tagaagtata
    32581 tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga
    32641 aaattattgg actgttgata acatacaccg tcatatatta aactattgta tatatggtgc
    32701 tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata aaaaggtagt
    32761 aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata gtcttaaaaa
    32821 gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg gtcataaaaa
    32881 acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt ataaggataa
    32941 agctgactta tatttttgtt tttataaaaa gattatagat attttaaagc aaggagggat
    33001 tggcagtgtt ataactccaa gatatttttt agaaagtcta tcaggtaaag atttaagaga
    33061 atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg caaatatatt
    33121 taaaaatata ggggtatcta gttgtattct aacttttgat aagaaaaaaa caaaagagac
    33181 atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat ttgaaacatt
    33241 ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga gattgctatc
    33301 tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga tacaagaaaa
    33361 atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta cagggtgtga
    33421 taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg ataaattttt
    33481 aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa gtgaatatag
    33541 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac tagatgaaat
    33601 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
    33661 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
    33721 tccttataaa tcaaatgaaa atagatttgc tatagattat gataacaatt tttcaagtgc
    33781 agatgtatat tcttttttta taaaagaaga gtatttagat aaattttcat atgaatatct
    33841 agttggaatt ttaaattcca gtgtttatga taaatacttt aaaataactg ctaaaaaaat
    33901 gagtaaaaat atatatgatt actatcctaa taaggttatg aagataagga tttttaggga
    33961 taataattat gaagagatag agaatttatc aaagcaaata atatcaattt tattaaataa
    34021 gtctatagat aaaggaaaag tagaaaaatt gcagataaaa atggataact tgataatgga
    34081 ttctttaggt atataagcag ttgtaagtat taaaaaatta taatctttta attgtttatt
    34141 taaatttaga aataaaaagt tagccatata aaaaatatta aaaatatata cttttcaata
    34201 tttttttgat aaaataagaa tgaatttgtg caattttatt tttctccaat aactaaaaat
    34261 agttttttga attaatattt gcttaagaat aaaaatcaga aacgtttttg atatagtagg
    34321 tgataatatg aaaggattag ttttagaagg tggaggaaca aaaggagcct accaaatagg
    34381 tgcatataaa gctttaagag agcttggtat agagtttcaa ggagtaactg gcacatcaat
    34441 tggtgcttta aatggagcat atataattca aaatgatatt gaagtaatgg aagatatttg
    34501 gttaaattat gactatactc attttatgaa tattgataga gaaacctatg agaggtttaa
    34561 aaatgtagat ttcactacta aaaatctcaa tgcagtgttt gagcttataa ataaagctag
    34621 aaaaaatgaa ggtatagata taagcccatt aagacaatta atggagacaa cattaaatga
    34681 agacataata aggaaatcaa aaaaagactt tggacttgtt acggtctggt gggataaaaa
    34741 aattaatcca tatccattgt atatcgaaga tatacctaaa ggaagattga ttgattattt
    34801 aattgctagt tccagtttgc ctatttttca aacgaacatg atggatgata agttgtatct
    34861 tgatggaatg ttttgtgata acatgccaat aggactcttg gaagacaaag gttatgaaga
    34921 tgttgttgta attaggttga ttgatgattt tattgggaaa atgaatctaa atcgacatca
    34981 aaatataaat ataaaaacta taataccatc agaatatcta ggaggttctc ttaataaaga
    35041 tagagataat gtagaaaaaa acatccgact aggttactta gatactatga aagcatatga
    35101 aagatgcact ggaattaagt attattttga tgtagagtat aaatataatg aagaatattg
    35161 ttttgataaa tttagaagta tgagcaatga aacaatacaa tatatctgta atttattgaa
    35221 tataaaaaga gatgcttcac ttagaactgt tatggaaagt gtaataccaa agcttggtga
    35281 ggtgcttgga ttgccaaagg atttttcata taaagatttg ttttactgta tatatgaaaa
    35341 gaaacttgaa gaaaataata taaacaggat aaaattatat gattttaaca aggttataga
    35401 tgttgtaaat acaaatattg attctaatag aaatttagct tctaattttg aagttagacc
    35461 taaaattagt attaaaaaga acaaaaacac aaaattattg acaaattgta ttataaaaga
    35521 cataaaaaat caaagttaaa taaaaaacaa aaaaaaattt gtaattatac tcgaaaaaag
    35581 tgtatattag tagtataata tttttgtagc actagtggga acaaaaaatg accataggac
    35641 aaaaaagtac cacttagtac cattaaagta aaaaacagga aaacaatttt aaaaaatata
    35701 aaatgtaaag tttataggag gatttaaaaa aatggaaaaa gtagttagta tcaaaaatgc
    35761 aagtggatta catgctagac cagcaggaat gtttgttaag aaggcagctg aatttaaatc
    35821 aacagtagag gttgtagcaa aagacaaaac tgtaaatgca aaatcaataa tgggaataat
    35881 gagtttaggt ttagctcaag gagaagaatt aaagttagtt gtaaatggag aagatgaaga
    35941 agtagcttta gctgcaatgg ttgaattagt tgaaagtgga tttggagaat agatataata
    36001 attttttaaa aatttgatat ttagaatcta atattactta aagtaaagta acagagaagt
    36061 attaaacata tacagaccaa catttgtaga tatttaattt ctttgttact ttcatgttta
    36121 tttaatcaga agattaagac tattgttaat tgggtataaa aatataaata ttttagaaaa
    36181 tgaaataaga tatttttaat cataaattca gagtaaaaat acagtaacaa atagtttata
    36241 aaattttata aaaaattata agtaaataaa actataaaaa tgtaaattat ttatataaat
    36301 ttataaagtt tatgtgctag atgatatttt ttaggaggta gggttatggc ttacaaagga
    36361 ataggtgctt ctcctggtgt tgctttaggg aaagcgttag tagttgaaca tagtgaatta
    36421 gtaattgaaa aaaaatctat agataatgtt gaagctgaaa ttgctaaatt agaagatgca
    36481 gttgcagttt ctaaagaaga attagtaaaa gttaaggaaa aggcttctga agaattagga
    36541 gcagaggaag cagaaatatt tgaagcacat ttattagttt tagaagaccc agagttaatt
    36601 ggttctgcaa tagataaaat taaaactgaa agtgtaaatg cagagtatgc tttaaatgaa
    36661 ataaaagaga tgtttgttag catgtttgag tctatggaca atgagtacat gaaagaaaga
    36721 gcggcagata taaaagatgt tacaaataga atacttagac atatattagg aattaaagtt
    36781 gttgatttat ctgctttaag cgaagaagtt gtattaatag cacatgattt aactccatca
    36841 gacacagcaa ctatgaataa aaaaatggtt ttaggatttt taactgatat aggtggaaga
    36901 acttctcata ctgctatcat gtctagaact ttagaaatag ctgcaatcgt tggattaaat
    36961 gatataactt caaaagttaa ggatggagat tttgtggttt tcaatggaga cacaggtgaa
    37021 gtaatcgtga atcctgatga agagactata aataaatata ctgaattaaa agctaagtat
    37081 gaagatgaga gaaaagcatt acagctatta aaaggaaaac catctgtaac tttagatgga
    37141 aaacatgtgg aattagctgg taacatagga acacctaacg atatagaagg tctaataaag
    37201 aatgatgctg aaggtgttgg tctttataga acagaatttt tatacatgga tagagattct
    37261 ttcccaacag aagaaataca atatgaagca tacaaagctg tattagaagg aatggatgga
    37321 aagcctatag ttataagaac attggatata ggtggagata aagaattaag ttatttaagt
    37381 atggaacctg aaatgaatcc attcttaggt tacagagcaa taagactttg cctagataga
    37441 aaagatatat ttaagactca attgagagct ttatatagag ctagtgttca tggaagatta
    37501 agaatcatgt tcccaatgat ttcatcttta gaagagttgt tacaagctaa agaagttgta
    37561 aaagaagttt tagcagaact tgattctgaa ggtgtggctt atgctaaaga tgtagaaatt
    37621 ggtatgatga tagaagttcc atctgctgct gtgatatctg atgtattggc taaacacgta
    37681 gatttcttct ctataggaac taatgattta attcaataca cttgtgcagt tgatagaatg
    37741 aatcaaaaga taagttatct ttacaatcaa tttaatccag ctgttttaag acttataaag
    37801 actgttatag acaatgcaca taaagaagga aaatgggcag gtatgtgtgg agaatctgct
    37861 ggagaccaaa aaatgatacc aatactttta ggtatgggat tagacgagtt ctctatgtct
    37921 ccaatatcaa tacttcctgc tagaaagtta ataacttcag ttaaagagtc agatatgaaa
    37981 aaattagctg atgatgtact taatatgggt actgctgaag aaataaaaag ctatatagaa
    38041 aagactttta atatataatt aatgttatat agttactaaa attcattttt ctaaataaca
    38101 aaattatatt ttacgaataa aaaaactata gtatatcata tatactatag tttttttgtt
    38161 atataaatta cgtatcaaaa tttgtaa
//
LOCUS       DAELJO010000013        38064 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELJO010000013 DAELJO010000000
VERSION     DAELJO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.013"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            398..580
                     /locus_tag="KPB88_000610"
     CDS             398..580
                     /locus_tag="KPB88_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0052918.1"
                     /translation="MEKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            869..1678
                     /locus_tag="KPB88_000611"
     CDS             869..1678
                     /locus_tag="KPB88_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0052919.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNEKYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTVLVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFANMLEELKVKGVL"
     gene            1679..2014
                     /locus_tag="KPB88_000612"
     CDS             1679..2014
                     /locus_tag="KPB88_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052920.1"
                     /translation="MPKFDEELLSNKEKPVFKKNGTIIQNNSTTTDNKTLNIYNLVNK
                     KSKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDVLTQILSDVTIKEENLNIYNER
                     NRRNKTKKK"
     gene            2157..2438
                     /locus_tag="KPB88_000613"
     CDS             2157..2438
                     /locus_tag="KPB88_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052921.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVANYLQYAGLDKNMKIKL
                     FQTILEMVARKKITLSSDSEFTEGILQSDEAEMTMNVGFIIEGLSYHNN"
     gene            complement(2741..3212)
                     /locus_tag="KPB88_000614"
                     /pseudo
     CDS             complement(2741..3212)
                     /locus_tag="KPB88_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3244..3747)
                     /locus_tag="KPB88_000615"
     CDS             complement(3244..3747)
                     /locus_tag="KPB88_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052922.1"
                     /translation="MNIDFSTILDLKVNKENVKMIEKEINKINNDIFDISLKDCANGH
                     IGSVKETVGKSLNIETWGHGEINSKIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIANVRPKFYKLEEKYSTRIEKCEIRHEFIGINKQEIKEFLEYLTEIEKSE
                     RTVIYKK"
     gene            complement(3824..4657)
                     /locus_tag="KPB88_000616"
     CDS             complement(3824..4657)
                     /locus_tag="KPB88_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052923.1"
                     /translation="MKILFKEAHKMAREIKRKYSNVDYKVQFSLCLSFLNKKGDIKLK
                     ELKGSEKQIIWANDIRTDILNLTNELEKSKIERIKNEDYKIKDMSIEEMTERCKRKFE
                     RIREAISNIENAKFFIDNFRNVLKYNSLNQKAFHINQILKDSQFAKENRNWSFLQGET
                     QASYRLKKVKDYSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTE
                     YIEKAKKYIIETLIQKNAQFYRNGFADIKKKQIENYRKIESDERKIEIAYQVFRDGVE
                     C"
     gene            4836..5048
                     /locus_tag="KPB88_000617"
     CDS             4836..5048
                     /locus_tag="KPB88_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052924.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            complement(5094..5933)
                     /locus_tag="KPB88_000618"
     CDS             complement(5094..5933)
                     /locus_tag="KPB88_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0052925.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKSEEKSYKIPKVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIIYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKISSMTKENYTMIKGNDRFETLYKALDF
                     IDR"
     gene            complement(5933..6364)
                     /locus_tag="KPB88_000619"
     CDS             complement(5933..6364)
                     /locus_tag="KPB88_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0052926.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPRKLKDRIGSLLDTMTDELNVKGGSK"
     gene            complement(6433..6615)
                     /locus_tag="KPB88_000620"
     CDS             complement(6433..6615)
                     /locus_tag="KPB88_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052927.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSGVVEGAEPTV"
     gene            complement(6615..6908)
                     /locus_tag="KPB88_000621"
     CDS             complement(6615..6908)
                     /locus_tag="KPB88_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052928.1"
                     /translation="MDRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(6925..8607)
                     /locus_tag="KPB88_000622"
     CDS             complement(6925..8607)
                     /locus_tag="KPB88_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052929.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQIMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYYPSTGGAGGGLEGGTGYSSNDRYR
                     PGGTQYQGGIGRVNTENGSFGKGCSVKDSTGEGGGGGWFGGAGMNGVGAGGGGSSYVL
                     TKDSYKPTGYTPTSEYYFDNIVMTPGGNTAGAYGYAQITLLQSLPFLNISSYNSTQAT
                     FKADHTDPTLLTKIEYFIDDVLKETITTDLTAEKTINYTVEDNALHTLKIVVTDSANA
                     TAEKVVSISKGIAPLPAGSTTDEVTSKWMEIKDTFKSGKTSIINTLALKNIEASLNNT
                     LVELSEKIKTSFDSSDASLQDLMNQLTQANNTISQLNSKYKVASGNSVVNYCTNTKGF
                     YFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDGYFHKYFVFACRGIFTQDFVITA
                     HYYRLTSYLQDFKVGGEVLKLNERDVYMDNRGIQLPCSRQGSSFKWQAIKFI"
     gene            complement(8625..9506)
                     /locus_tag="KPB88_000623"
     CDS             complement(8625..9506)
                     /locus_tag="KPB88_000623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052930.1"
                     /translation="MEKSFVFNSVNGDRRYKAEDFREYFASFIGNGVFPNPSSNLQVV
                     DNNNMTITVKKGKGWINGAIYINTDDLVINIDPADGILNRIDRVVLRFDTLNRNIKLA
                     VKKCTFNSSPVATELQRDADAYELGLADIYIRAGAISITQSSITDLRLDKNLCGIVKG
                     TIEEIDTTTLLAQLNTWKDEEISNFNVWRENQENKQEDWYNTTTTNFVSEFNIWFESI
                     KDILDEDAAGNLLNEINKVKEELAKIETTAEKTSYNNATSNLTATNVQGAIDEVVRKI
                     EKFNEVNISIQNDMLPI"
     gene            complement(9506..10585)
                     /locus_tag="KPB88_000624"
     CDS             complement(9506..10585)
                     /locus_tag="KPB88_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HBG0052931.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKKFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLILHREFMYTETGEKSDILSVKGVDLKGLLNRRLII
                     PDTGEAFESHEGTQEEIIKAFVNNNCVDPSNPKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEISEFCGLGWEVVLDANKKKFIFDVITGKDLTINQTENPPVIFRSDFNNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISTGEVEGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFMYEKDYKLGDIVTVQDRKLKVTMDTRIVEVQESYSK
                     NGMKLKITFGSSIPTLFSKIKRMVK"
     gene            complement(10587..11453)
                     /locus_tag="KPB88_000625"
     CDS             complement(10587..11453)
                     /locus_tag="KPB88_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBG0052932.1"
                     /translation="MQKLICRNSKGQEVKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPISAAIYAKNKEDLELKRAYLTSLFNPKLGEMEFIYTNNALTK
                     RVKGVVQDITFQTPVRLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVVNNGDVECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            complement(11486..16687)
                     /locus_tag="KPB88_000626"
     CDS             complement(11486..16687)
                     /locus_tag="KPB88_000626"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0052933.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGVNNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSAGKQMQDVGKKMTMSITA
                     PILAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMEKLGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALMSA
                     IEELGLKIYDALKPSIAQAVSWFQNLTNKLNEMKPSTVETIVKIAGLAAALGPVLLIG
                     GKIIGMIGGVVGAFGTFSAAMAVVTTGAAAATPAVGALAAILGALSSPAAVAIAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSVSESTKEAVGAFMDLEEKTTISLNQLMWSGET
                     VSEKMKQSIVSNFTEMSNQIIAKLQESKEQGIQSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQEHRTLTKEESAEINQIKTDMLNTAVDTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRTEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQANKVDWTTGEIKSK
                     WQVLNDTVKGKMQEWDDIAGNWVEGLKEKISIGWANLKENTVLLWDNIKLTVTNKWTE
                     IKESISLKIEEIKQGISEKWESIKLFFQTTWESIKLIFSNAWDAIKQQLEEKFAPFIE
                     KLKLIFDSIKELFNNVWEVIKNVVLGAVLLILDVVTGNFDKLKTDALQIWENIKQGLS
                     NTWESIKEVFNNALAAIGDLLSGMWESMKSTAITIWESITQTLSNIWENIKSTCSTAW
                     EGLKTICSNTWENIKSTLTNAWESMKTAVTTKLGEIKNNIVTTWNNVITFFKTLPAKL
                     KQAAIDMFTRLREGIDSKKQDAVNASQKVGTDVVNKIKEFPSKFLQVGKDFVSGLANG
                     ISGFAYKVVDEAKKLGQKAADAVRKVLGIHSPSRVMAEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEEDIKLFNKQIDELSKQEKAALKKVKGDAKDALKEE
                     YAAKKEKIKEAIALRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAQQASIKKELDALDKWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKVLAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKLIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            complement(16756..16974)
                     /locus_tag="KPB88_000627"
     CDS             complement(16756..16974)
                     /locus_tag="KPB88_000627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052934.1"
                     /translation="MCKYCEVKINTEPFSMYDTVVRGELLTNDSDIDMYIELKNGKHF
                     LTGASYDYLGISSKETIKYCPFCGRKLI"
     gene            complement(16987..17142)
                     /locus_tag="KPB88_000628"
     CDS             complement(16987..17142)
                     /locus_tag="KPB88_000628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052935.1"
                     /translation="MFLFEKNWSMTDIDNMDIFYYLDILVYMKENKKDNNNPNENDVY
                     IDQISWL"
     gene            complement(17226..17513)
                     /locus_tag="KPB88_000629"
     CDS             complement(17226..17513)
                     /locus_tag="KPB88_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052936.1"
                     /translation="MEIKLTINEEEKIFKAPPIALRKLDKVFTLTDKIEDGLNSTELF
                     RELLDFTVDIYGEQFSKDELLDGFYPAGDFINKALEDLSKVSGGFEEKVKN"
     gene            complement(17528..18124)
                     /locus_tag="KPB88_000630"
     CDS             complement(17528..18124)
                     /locus_tag="KPB88_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0052937.1"
                     /translation="MAIIGLRNYRIVKLLTDTEETLEYDTKITRLTGAKSVKISPKVD
                     SAENYGDDQLLETASAMGAIEVEIEVADLTLEERALILGYQYKDGVLIEDKNFAPPNL
                     AFGFESPKSQNGSRMVWLTKGVCEPFEEEAKTKEDKIEFQSQKIKLKFMPRIHDGRHK
                     ITADTDVENAPTNAQFFTTDFLKTGVKPTAPAKANLNK"
     gene            complement(18128..18463)
                     /locus_tag="KPB88_000631"
     CDS             complement(18128..18463)
                     /locus_tag="KPB88_000631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052938.1"
                     /translation="MININDLVIETLKPLKIPVSFQRFDGEEKTYITFFSYLEQNRYA
                     DDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTIFRF
                     LFFAEKKED"
     gene            complement(18463..18906)
                     /locus_tag="KPB88_000632"
     CDS             complement(18463..18906)
                     /locus_tag="KPB88_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0052939.1"
                     /translation="MSVEITTEGFDAILSKIESMGKTGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSEIFYGKFLEFGASAHKIPIK
                     KGKKKGRVVNHPGTSSKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            complement(18907..19233)
                     /locus_tag="KPB88_000633"
     CDS             complement(18907..19233)
                     /locus_tag="KPB88_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052940.1"
                     /translation="MHSKIVTILKKLNLGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVV"
     gene            complement(19253..19903)
                     /locus_tag="KPB88_000634"
     CDS             complement(19253..19903)
                     /locus_tag="KPB88_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HBG0052941.1"
                     /translation="MKKIKFNNFNDGIVEFGEYKESYDIEGNALDEKEFIVSGKLFYS
                     NEYIREQDKLKFDDTGLKVSIKLKVPYMRLIKTSDVIKLNNEFYSIVKLDISNDKTSV
                     YLYLTELVDVLDKHISIFIKERKNVLEDVTIKFYKKIWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            complement(19906..20193)
                     /locus_tag="KPB88_000635"
     CDS             complement(19906..20193)
                     /locus_tag="KPB88_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052942.1"
                     /translation="MENENIKSVVEEVKEYLNITWIEEDAKIEKMVVRGIDYFENEIA
                     GIALDFKDNFNRELLFNYCRYVRNNAIEYFEENFSKELLRLQIKSAANEVI"
     gene            complement(20203..21369)
                     /locus_tag="KPB88_000636"
     CDS             complement(20203..21369)
                     /locus_tag="KPB88_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBG0052943.1"
                     /translation="MAIKNLDEATNVELRADLMEAIKGNDEEKITTAFLRMAEDIQEN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSKVIEKRGFTNLEITMPRTVFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWIIRKTNAEAAWWGRLDDTIKKELEHGFDKINT
                     EAYKLSAYLPVAKAMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPKGMIKD
                     LKGAVVEGVFPDKTAKPLADFKPITLGKEIMAPLTNNGKRAVPSVIMLVNPVDYWAKI
                     FAATTMLTSNGTYVYGVLPIPGNIIQSVAVPKGKLIAGMAKDYFMGVASSGKIEYSDE
                     YKFLEDERTYLVKQYATGAPKDNDSFLVFDIENLDTTLATEINSNTKTSAARAK"
     gene            complement(21372..22007)
                     /locus_tag="KPB88_000637"
     CDS             complement(21372..22007)
                     /locus_tag="KPB88_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBG0052944.1"
                     /translation="MEMEKRTVSLEIRDSNLESRKISGYAAVFSDDYTKLQDRWGDTF
                     YEKISRGAFLKTLADNTRDKFMLINHDWNKVVGRTNSNLILEEDEHGLRFEVDIPATT
                     DGNDLLENVRLGLIKGCSFGFNIVNSKTRWDDDWTFYRDITEVELFEITATPIPAYND
                     TEINCRSDISIKELRESQKEETKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            complement(21964..23151)
                     /locus_tag="KPB88_000638"
     CDS             complement(21964..23151)
                     /locus_tag="KPB88_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0052945.1"
                     /translation="MNFKNFISNFLFKDKMSGADGEYFKKLEATIFYKEFALKSCISI
                     IANALVLSEFQTFKNGKSIKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQ
                     ENEQLFVAESFSVDKYVFYEDIYKDVTIRNYKLDRSFRESDVLYFKLNVESIKTVIDY
                     LYNDYKDLLSSAMKGYKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDN
                     AVLPLSNGLKYTENTKASSIKDSRDIRAIIDDVMDFVCAAFHVPAGLVKGNVAGVEGI
                     TDNFLNFSVNPVAKLITNELTRKMYGRDNFFEKSYVKVDTQKIANMGLEKISKASDLL
                     FRIGVNSINDNLEMLGREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKNGVAG
                     NKG"
     gene            complement(23167..24795)
                     /locus_tag="KPB88_000639"
     CDS             complement(23167..24795)
                     /locus_tag="KPB88_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBG0052946.1"
                     /translation="MILNYKYNKYIDEYFSLADKGEIVLCKEMKLAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     IFYITTDGNVRGGVLDDFKEEAKQILNEEIKNSKMLPLIFKLDSIEEMEDVNLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAEDLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFTNKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDDELMQESTIKFYDCYTY"
     gene            complement(24779..25285)
                     /locus_tag="KPB88_000640"
     CDS             complement(24779..25285)
                     /locus_tag="KPB88_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HBG0052947.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            complement(25457..25789)
                     /locus_tag="KPB88_000641"
     CDS             complement(25457..25789)
                     /locus_tag="KPB88_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG0052948.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEIKEYPSEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            complement(25779..26051)
                     /locus_tag="KPB88_000642"
     CDS             complement(25779..26051)
                     /locus_tag="KPB88_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052949.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSYEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            complement(26224..26772)
                     /locus_tag="KPB88_000643"
     CDS             complement(26224..26772)
                     /locus_tag="KPB88_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0052950.1"
                     /translation="MNFVEPIRDLEKLESICAYLEETNMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITLKESKTGKTKPIEVNPILRRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            complement(26953..27414)
                     /locus_tag="KPB88_000644"
     CDS             complement(26953..27414)
                     /locus_tag="KPB88_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052951.1"
                     /translation="MKIKKEYFDKVENALKMLGDKYRKLRVLKKEIAILKHKENYREV
                     NYEELGFKVQKSVKGIDDMVVTIEDAIYNKETEIEIIERKLEFYNIYLKELSEIEQKI
                     IELVYFYSWDGKMPITKMARELNYDRTSLYNKKAIAINKIALMIYGDEALE"
     gene            complement(27657..27866)
                     /locus_tag="KPB88_000645"
     CDS             complement(27657..27866)
                     /locus_tag="KPB88_000645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052952.1"
                     /translation="MSMCRKCLCGDYLDELVVGECILCGQEFFSIKTPVGKMCPSCSE
                     MFKGNCSQCAKPIGNEKKLFKNKLK"
     gene            complement(27884..28144)
                     /locus_tag="KPB88_000646"
     CDS             complement(27884..28144)
                     /locus_tag="KPB88_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052953.1"
                     /translation="MNFKMIANIPELTAKEQFEKVLEEVLELKEAIELKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKEIREGLKLHNKKLRKRKWKLEKIN"
     gene            complement(28162..28437)
                     /locus_tag="KPB88_000647"
     CDS             complement(28162..28437)
                     /locus_tag="KPB88_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052954.1"
                     /translation="MGKTTIEEFLTKIKEEIQCTTKESIKDLENILKEIEIGETEVRI
                     LFIDKKNKIGEIVKFTHDEYMKEIIRKMILEGLDAIKKQHEEILEEI"
     gene            complement(28439..28687)
                     /locus_tag="KPB88_000648"
     CDS             complement(28439..28687)
                     /locus_tag="KPB88_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052955.1"
                     /translation="MKEYRIEIEETIFLKHKVIIEVPEEMCILDILEYVENECQDKSE
                     ISEYIEKNGGKQLNFIEGKRRDSDFTVESCRRIKKGEK"
     gene            complement(28674..29413)
                     /locus_tag="KPB88_000649"
                     /pseudo
     CDS             complement(28674..29413)
                     /locus_tag="KPB88_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
     gene            complement(29410..29559)
                     /locus_tag="KPB88_000650"
     CDS             complement(29410..29559)
                     /locus_tag="KPB88_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052956.1"
                     /translation="MSNINKKTAKICGWCGTTIYSYTDGKIAYCSKKCKKKREKNLET
                     EGAKK"
     gene            complement(29576..29983)
                     /gene="ssb"
                     /locus_tag="KPB88_000651"
     CDS             complement(29576..29983)
                     /gene="ssb"
                     /locus_tag="KPB88_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0052957.1"
                     /translation="MNNVAMIGRLTKDPELKYIPGSGTAVSTFTIAIDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLTKGRLIGVEGSIRVNSYEKEGEKRTYTKVHANKIKFL
                     DYKKEDNEKEYMFEPKGLDQQGFQAIDDPDIPF"
     gene            complement(30015..30230)
                     /locus_tag="KPB88_000652"
     CDS             complement(30015..30230)
                     /locus_tag="KPB88_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052958.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYKKEYRGKFYDWIKSLE
                     RKDSLQNYKIVEVLNDERNGEEAPFWN"
     gene            complement(30223..30390)
                     /locus_tag="KPB88_000653"
     CDS             complement(30223..30390)
                     /locus_tag="KPB88_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052959.1"
                     /translation="MDKFVTCARCERTINVEENNYVKYEEETLNLSFALYFCLGCVDK
                     IIEEQVEGENE"
     gene            complement(30405..30710)
                     /locus_tag="KPB88_000654"
     CDS             complement(30405..30710)
                     /locus_tag="KPB88_000654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052960.1"
                     /translation="MNINKYEKLCEQQELLYEQYKETKEKIKVYEVLEDRRKGIDTMI
                     RNIDSYFGIGELSMEFNIRGSCDVSCVHFSVGEEEKILLKNFFIELKEKNVRKMEEI"
     gene            complement(30740..31105)
                     /locus_tag="KPB88_000655"
     CDS             complement(30740..31105)
                     /locus_tag="KPB88_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052961.1"
                     /translation="MNIGSYTLVKKQLIEELEEGNKQKEEYLKECEKHIKGLEKSLEN
                     FARDALESEECIIDIENILIEQALELETYNLFIDCILNKNIRKAIAIYNRTKSIRIKN
                     KNINSISNKIEKIKQIISL"
     gene            complement(31102..31266)
                     /locus_tag="KPB88_000656"
     CDS             complement(31102..31266)
                     /locus_tag="KPB88_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052962.1"
                     /translation="MQKKISEMSILEKADLISKKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKF"
     gene            31355..31699
                     /locus_tag="KPB88_000657"
     CDS             31355..31699
                     /locus_tag="KPB88_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HBG0052963.1"
                     /translation="MVTNQDCIRDILLFIESNTTPISPVLQFDTILDNLKYDESTIIH
                     HINLIYQNSLVDDVNYGDDTVLEVTQLSLKGYQYLDEIRDSTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            complement(31742..32023)
                     /locus_tag="KPB88_000658"
     CDS             complement(31742..32023)
                     /locus_tag="KPB88_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052964.1"
                     /translation="MIKNNVLSYEEKIKVLKEHGYNDSTKEYILKEFGKEHIFYESFL
                     RAIERDDSKKSVLIKKFEKGQHQFSEGFIKTHTISELLKEEERIRKILG"
     gene            complement(32069..32671)
                     /locus_tag="KPB88_000659"
     CDS             complement(32069..32671)
                     /locus_tag="KPB88_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052965.1"
                     /translation="MEINSVRELKELVELFGVIKAESNNVVSCNETVNELSNEELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            complement(32694..33179)
                     /locus_tag="KPB88_000660"
     CDS             complement(32694..33179)
                     /locus_tag="KPB88_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0052966.1"
                     /translation="MLSNKTNINKTNLTNNIYSSAEQKEEKTKDKEIYKRIVDYLNKT
                     ADKSFKSTTKKTISLIDARLKEGFTEEEFYKVIDNKVLNWLDDDKMNAYLRPETLFGN
                     KFESYLNETPKKALKGNESVKGKTKTNNNKFANFDQTFNKYSEKELDAIIKKSQSVKF
                     K"
     gene            complement(33600..33815)
                     /locus_tag="KPB88_000661"
     CDS             complement(33600..33815)
                     /locus_tag="KPB88_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052967.1"
                     /translation="MKLLKSKNIKIGEIIRLHDVKFNHDRKCIVTYIDKDEIVLTYYD
                     EEIKEMTCKVLTKEDLIFNDYELKLLS"
     gene            complement(33852..34016)
                     /locus_tag="KPB88_000662"
                     /pseudo
     CDS             complement(33852..34016)
                     /locus_tag="KPB88_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            34245..34589
                     /locus_tag="KPB88_000663"
     CDS             34245..34589
                     /locus_tag="KPB88_000663"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052968.1"
                     /translation="MNIEERLKQLILSKYKSIRAFTKEVNIPYSTVDTMLKRGISGTS
                     ITTVLKICNALNIDADALIDNELKEKELSSKIQTNQFEQKILKMYNALDEHGKDIVFT
                     ILEKEYVRINKL"
     gene            34772..35071
                     /locus_tag="KPB88_000664"
     CDS             34772..35071
                     /locus_tag="KPB88_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052969.1"
                     /translation="MESLLKYHRKKKKLTQRRLSELTGISQSYISRMENKKFKHSPTL
                     TQIILLADILEIDQLILSEFFLEKEREHLKNTKKYNKKAVKTLSRDFLKEYKNKD"
     gene            complement(35099..35773)
                     /locus_tag="KPB88_000665"
     CDS             complement(35099..35773)
                     /locus_tag="KPB88_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052970.1"
                     /translation="MMLKYITKHKNMEESRMKKVLLLITVCLLSVGLVACSSTNNKDT
                     GNTTKKEKKANENEVDGIKITVKDVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAAD
                     YVYEVVNVEVENKTDKAVKLFQTGWNAVGTDGYEFKDIKVTDKLDNQQVPANYKFDAQ
                     VKILVEKNMNVKEIVLKYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELG
                     EIKVEQ"
     gene            complement(36389..37081)
                     /locus_tag="KPB88_000666"
     CDS             complement(36389..37081)
                     /locus_tag="KPB88_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052971.1"
                     /translation="MSLEEAKELEKNYKLPMPTYCLNEKNYCAKEFGALMLISKGSMR
                     IMHIEKNRYLYQNKFKMEELAKQIGVARTTLERNIKKLNSLDCKVLEIENSRNGIIYR
                     LNYGTSTGYNDNVHKFVTIHHDMLQELISAFNTNAIKVYCLLCYMTTENSFKCMTEKF
                     ICEKIGLCGDSKNNRSKIRKIITVFEVSKYIEVKKENKFEWDEEKNKKVPRIQKLYRL
                     CSFSEWKNARKK"
BASE COUNT        11312 a         6841 c         4235 g        15676 t
ORIGIN      
        1 aaaaaaaaat aaaagaaagg aggaacggga atggggagat tagaaagaag taaaaagaaa
       61 agagaaaaca aatttaacat agttaaaaaa gttttctctt tcatcctatt attacttaac
      121 ataatcctcg cagttctaag aattttaaaa gaactgtaag gataagccta gagggaatag
      181 cccttctctc taggtgactt ctttctaata ttataacaca tttctaaaaa acatgagtga
      241 taaattttat aagatttgca ttgttttagt tttgataact atagtcttaa atatagtatc
      301 tatagtatta aactctagta caagcaacat gctcggttta gtttttagtg ttgtattatt
      361 gcttttcttt gtaatacagc aaaaaaggag ctaatgaatg gaaaaagaaa aaagagattt
      421 aaagtttaac tttcacaaag gtgggagtgg gtcgtttact cctagaatga gtgttcctaa
      481 aaaatgggct gatgaaatgg gcataacaag agaaaaccct aatgttacgg caacattcga
      541 gggcgacagg ataataattg aaaaggctaa gaatgaatga ctttttctat aaaagaaggt
      601 tgcttttttt cttagccttc ttttttttaa attttactat aatataaaaa taatactgta
      661 atattataaa aatattattt tagtattgta tatacaatat tttagtattt ttaatataat
      721 atattggtat tgtaaaagca atatgtaaat attgtattta tattatttga atattgtaat
      781 taacaatatt aatatattgt tgcaatatta taaaaacaat actacaatat tgttataata
      841 ttataaatat aataatgagg tggagaaaat ggctaataca aagctactta cgtactttaa
      901 cataaaagga ggtatctata agacaacaac gtctataatg acagcgtatg aacttgcgaa
      961 agataaagat aaaaaaatac tcttatggga cttagatgta caagcaaatc taacacaata
     1021 tgtttatgaa ataaatcata atgataatac tacattagat attttaaaag ggattagtgc
     1081 taatgatgca atagtcaagt cacctaatga aaagtacatt aatgtagact taattccttc
     1141 tgacatacaa atggctagat ttgaacaaga gctatcacct ttaccagcca gagaaaaatt
     1201 tttagctcgc tggtacatgc aaaactttaa tactttgagt gagtatgatt atattatctg
     1261 tgatttatct ccacgctacg atttgacagc taaaaatgtg cttttcttag cagatagtat
     1321 aattattcca attcaagata aaaacatttc ttctttaaga ggagcagaat tatttaaaca
     1381 actctgggat gttgatagaa cctattttga taaggaggat aatataaaaa gtactgtttt
     1441 ggttggattt gagaagaaga aaactcaaat tagtgatact ttcgattctt atctggaagg
     1501 gtttaacgat atgagagata ttatgttaga tacatatatt agaaaaaacg agtttataga
     1561 gaaggcacta ttgaaaaaac tatctctaac agattataca aaaataacta aagagcattt
     1621 cagcagacaa gagtttgcta atatgttaga agaactaaaa gtgaaaggag tgttgtagat
     1681 gccaaaattc gacgaagaac ttttatctaa taaagaaaaa ccagttttca aaaaaaatgg
     1741 aacaatcata caaaataatt ctacaactac agacaataag acattaaaca tatataatct
     1801 agttaataag aaaagtaaaa aggtaactat gagtgcaacg cttgatgaag aacttgtaaa
     1861 caaattaaaa tctttttcta ttgatatgaa cagcgacgtc agcaaattat taagtgatgt
     1921 actaactcaa attttatctg atgtcactat aaaagaagag aatttaaata tatacaatga
     1981 gagaaataga agaaataaga ctaagaaaaa gtaaataata ttatatttat attgtttaga
     2041 tattacttaa atattgtata tataatattt aagtaatatt ttaatattat tattacaata
     2101 tatcgatatt gtttaaataa tatgttttaa ctataatcta aaaggggttg gaaaagatga
     2161 atgtttattt gcaaattgga aaagagttat ctgaaaaaat gactaaatct gaattaaata
     2221 agttttcgca ggaggtggca aactatctgc aatacgctgg acttgataag aatatgaaaa
     2281 ttaaactatt tcaaacaatt ttggaaatgg tggcaaggaa aaaaataaca ttatcaagcg
     2341 atagcgaatt tacggaagga atattgcaaa gcgacgaagc agaaatgaca atgaacgtag
     2401 gatttataat cgaaggatta agttatcata ataactaaac atttttaaaa aaacagtgta
     2461 tttattgaat acactgtttt tatttccgca tagttaatct aaaattggat attgtatcat
     2521 atggatatag attaaattga tatttaaatc ccttatagtt aatcttaaaa attgttatta
     2581 tattatttct ctataaatta aattgacatt taaatccctt gtagttaata taaaaattct
     2641 tattacaata tcatttctaa ataaattaaa aaatatttta aatccctcgt agttaatctt
     2701 aaaagcgata ctatatacat taataataat actcttctac ttaattttca actatatttt
     2761 gtaattcttg tttacaactt tcaatgcctt ctcgtaattc ttctaaaaaa aactgatgtg
     2821 ctatcgtcta tatttctttc agcaatttct tcaaaatttt ctttattgaa ttttttataa
     2881 ctatcccaat tcaaactttc gcatttattt tcgcaccaat tttcaaattc ttcgtaatca
     2941 tttacgtagt aaatttctcc aaacatatcg tctaagttat ttcctgtttg tcttgatgtg
     3001 aaaatattaa tttctttatc atcatctaaa agtttagcat aagcctggat tgctatcgct
     3061 tcaacttttc cttgtatatc gatataaatt tttatatcat tagtctcttt gattaaacaa
     3121 ctttctttaa aagcttttat agcttcttcc tctattttat ctatattcat atctataacc
     3181 tttaaaaatt ttttagcttc ttttttatcc attttatcca ttttaatttc ctccattttt
     3241 tagttatttt ttatatataa cagttctttc gctcttttca atttctgtaa gatactctaa
     3301 aaattcttta atttcttgct tgtttatgcc tataaattcg tgtctaattt cacatttttc
     3361 aatacgtgta gaatatttct cttctaattt ataaaattta ggtcttacat tggctataag
     3421 gtcactttct acatttttgc tacacaagct atttacatat attaaaatgt tttctatatt
     3481 ttctatgtct actttgtcgc cttttgaata ataaaaatta aatctcaatc ttattcctat
     3541 tttactgtta atttcaccat gaccccacgt ttcaatgttt aaactttttc caactgtttc
     3601 tttaacgctt ccaatgtgac cgttcgcaca atctttaaga cttatatcaa atatatcatt
     3661 gttgatttta ttaatctctt tttctatcat tttaacattc tctttattaa cttttaaatc
     3721 taatattgta ctaaaatcta tattcatgtt ttaaacctcc aattattttt tattttaatt
     3781 atagcattca atggtgaact atatttgtat caagttgaaa cttttaacac tctacgccat
     3841 ctctgaatac ttggtatgca atttctattt ttctttcatc actttctatt tttctatagt
     3901 tttcaatctg cttcttttta atgtctgcaa atccatttct ataaaattgt gcattttttt
     3961 gtattaatgt ttctattatg tattttttag ctttctctat atattctgtg ctagtagctt
     4021 gttttactgc gtcgtcaaat atttcaacta tttcaagtac attttctttt atttcattag
     4081 accaattatc attaaaattt atttctttag ctattttctt agcttcttca tagcttatac
     4141 tataatcttt aactttttta agtctataag atgcttgtgt ctccccttgt aaaaaactcc
     4201 aatttctgtt ctcttttgca aattgagaat cttttaatat ctgatttata tgaaatgctt
     4261 tttgatttaa agaattatac tttaaaacat ttctaaaatt atctataaag aacttagcat
     4321 tctctatatt gcttattgct tctcttattc tttcaaattt tcttttacat ctttctgtca
     4381 tttcttctat actcatgtct ttaattttgt agtcttcatt ttttattctt tctattttac
     4441 ttttttctag ttcgttagtt aaatttaaaa tgtctgttct tatatcatta gcccaaataa
     4501 tttgtttttc tgaccctttt aattctttca attttatatc ccccttttta tttaagaatg
     4561 ataagcaaag actaaattgt actttataat ctacattact atattttctt tttatttctc
     4621 ttgccatttt atgcgcttct ttaaataaaa ttttcattct ttgcttaccc ccttgtttta
     4681 tttgttaatt atattataat ctatatgaat atctaagtca acactttttc aaagtttttt
     4741 ctttgatttc caaaaatata actttgatta gcgtagatta ttcttttact tttaattttt
     4801 ttatgttatt atataaataa gagaggtgcg aaaaaatgac atggaagaat gaaattaaaa
     4861 gtattcttgt aaaagaaaat atcagcatga atgagttaaa taatttaatg aatgaacata
     4921 ataataagaa taatacagtt gaaaatttaa gacaaaaaat aaataatgag actatgaagt
     4981 atagtgaaat cttgactatt gctaatttga taggatatga agttgtttgg aaaaagaaag
     5041 aaaagtagac ataaaaaaag aaggtaacaa ctaactagtt gctaccttct aatctatcta
     5101 tcaataaaat ctaatgcttt gtaaagtgtt tcaaacctat cattaccttt tatcatagta
     5161 taattttctt tagtcataga acttatcttt tcacatgctc caccaccaac aacataaaga
     5221 ttttgtgtct ggccaggtac ataatctttt atatcacata ttaatatttt cccatcatta
     5281 taaccccatc caacaacagt tgcagggatt ttgtcaactt ctccatcata aatgattgta
     5341 tgtttgtaca tctgtttaac tccctcatta tttatatttt tatttaatac accttctaca
     5401 atcaacttag caataccttc atgacctagt ttcttagctt tctcataatc ttctttgtta
     5461 tcgcaaaaga aactttcaat tagtattgca gtaggtttag aactatttaa aatatataaa
     5521 cttttatcta atttaacacc tctattacct acttctttat ctcctttttt tcttatgaaa
     5581 ggtttagata atttatctac taccctttgc gcatattcct tccctttttc actatagtaa
     5641 aatacttctg ttccaaaggc tcctacacca cttgaattta aatgcaactc tataagtaag
     5701 tcatatcctc cactattaac tttaggtatt ttgtaagatt tttcctcact cttagtttta
     5761 aactgctttt cggggcatat tattacatct gccttatgac cttcttttct aaaagtatct
     5821 gctaatactg gtgcaagaga tttattgtat tgatactcgt taactactcc attagcagaa
     5881 gtacatgccc cgctttttaa aatgctgtgt cctactgtta tacatatttt cattatttac
     5941 tacctccttt aacatttaat tcatctgtca tagtatctaa taaactacct attctatctt
     6001 ttaattttct aggtactggt aacccacaca aatacatgtt ttttaatata cttacacttt
     6061 catatagaat aaataaaata gagaaaaatt cagatattcc aagatgattt aatcgcaaaa
     6121 aatcaaccca atcttgtggt aacataaata aaaagttaaa ctttgtaaga atgtcaacta
     6181 ctgctagaaa aaatatacat gctatcattg caacttttct tattcctcca tttattccaa
     6241 aacttgaatt aaactgatgt gtttttattg ctcttaaaca acctagcaat gtatcaaatg
     6301 ctattgctaa tattactaat tttataaata tatttgtcgc taaaaaaact attgttacgt
     6361 tcatatttcc tccttatttt gcattaaaat aagacttaga attatctaag cctatagaaa
     6421 aaggaacatt acctatactg taggttctgc tccttctact actccactat gttctataat
     6481 gtagttttca actgccatcc tgtactctgt gttagtaaca tcatcaagtt caaatactct
     6541 attttttaga ggatttaatc ccttatttaa tattctctct gctaatattc ttactacaac
     6601 attatttata ttcattataa aattcctcct accttttcat tttctgcaat tagtaattga
     6661 ttttctaact cttgtattct cttttcttca tctgaaataa atgttggtat ttcctctaaa
     6721 attggttctt ttgtttctat atttatacct ataattctat ttcttgtata gtctatactt
     6781 ccatactcaa catcaatata atgtaattct gttattgtat catgttctag tatatctcct
     6841 gttgcttccc ctgtctgtag cagtattttg cctgtctggt cgtaaattat tctatttgct
     6901 ctatccattt tatcacctca tttattaaat aaattttata gcttgccatt taaaagaaga
     6961 accttgtctt gaacaaggaa gttgtatacc tctattatcc atataaacat cacgttcatt
     7021 taatttaaga acctcacctc caactttaaa atcttgtaaa tatgatgtta gacgataata
     7081 atgagcagta attacaaaat cttgagtgaa aataccacga caagcaaaaa caaaatattt
     7141 atgaaaataa ccatcatcaa ttaattcaaa agtagtaaaa aatatattag gaacaaaatt
     7201 aagtccgtta atttgaatgg ctccaggaaa taggaacaga tagtcactat taaaataaaa
     7261 tccttttgta ttagtacaat aattaacaac actattgcca ctagcaactt tatatttaga
     7321 atttaactga cttatagtgt tattagcttg tgttaactga ttcatcaaat cctgcaaact
     7381 agcgtccgaa ctatcaaaac ttgtttttat tttctctgac aactctacta gcgtattatt
     7441 taaacttgct tctatattct ttaatgctaa agtgtttata atacttgttt tgccactttt
     7501 gaatgtatct ttaatttcca tccatttgct tgtaacttca tctgttgtag aaccagcagg
     7561 aagaggtgca attcctttgc ttatacttac aactttttct gctgtagcat tagcactgtc
     7621 tgtaacaact attttaagtg tgtgtagtgc attatcttct actgtatagt taattgtttt
     7681 ttctgctgtt aaatctgttg ttatagtttc ttttagtaca tcatctataa aatattctat
     7741 cttagtcaat aaagtagggt cagtgtggtc agctttgaat gttgcttgtg tagaattata
     7801 agatgatata tttaaaaatg gtaatgattg aagtaaagtt atttgtgcgt aaccataagc
     7861 accagcagta tttccacccg gtgtcataac aatattatca aaataatatt cagatgttgg
     7921 tgtgtagcca gtaggcttat aactgtcttt agtcaataca taactacttc cacctccacc
     7981 tgctcctaca ccattcatgc ctgcaccacc aaaccagcca cctccgccgc cttcgccagt
     8041 tgaatcttta acagaacacc cttttccaaa acttccgttt tctgtattta cacgaccaat
     8101 accaccttga tattgagtac caccgggacg gtatctgtca ttagaactat acccagtacc
     8161 tccttctaat cctcctcctg caccaccagt ggaaggataa tatgaacctc cgccaccacc
     8221 tgcgacaatt atacgagata gcaaaccttg ctcattatcc caagcaccac caacaagtct
     8281 tatatcggta gcaccaccac cgtacataga ataataagta cccataatct gttgatttaa
     8341 ataaccttta ccaccgccat taaaaccact tttagtgtta ttactcgtag atgaagaagc
     8401 aaaaccactt tcgccgacgt aaacatataa tgtagtttgt ttttttaatg taatttcacc
     8461 tttagaatat ccgcctttag catcagtata ccaagaagaa ttgttgatac ctccagaaga
     8521 accccagcat tcaaatttat atttgccagg tttcaatata acactttgtg gcgaaccatt
     8581 gtacccaaag ttccattctg tctgcatttt ctcactctcc tctcttatat aggtaacata
     8641 tcattttgta tagatatatt aacctcatta aatttttcta tttttctaac aacttcatct
     8701 attgcccctt gcacattcgt agcagtaaga ttacttgttg cattattata acttgttttc
     8761 tctgctgttg tttctatttt tgctaactct tcttttactt tgtttatttc atttaataag
     8821 tttccagctg catcctcatc caaaatatct tttatacttt caaaccaaat gttaaactct
     8881 gatacaaaat ttgttgttgt agtgttatac caatcttctt gtttattctc ttgattttcc
     8941 cgccaaacat tgaaattact tatttcttca tctttccaag tatttagttg tgcaagtaat
     9001 gttgttgtgt ctatttcttc tatcgtgcct tttacaattc cacacaaatt tttatctaat
     9061 cttaaatctg ttatagaact ttgtgtaata cttattgctc cagctctgat ataaatatca
     9121 gctaatccaa gttcgtacgc atctgcatca cgttgtaatt ctgttgcaac tggggaacta
     9181 ttaaaagtac attttttaac agctaattta atatttctat ttaaagtatc aaatcttaaa
     9241 actactctgt ctattctatt taaaattcca tctgcggggt ctatattaat tactaaatcg
     9301 tctgtgttta tatagatagc tccgttaatc cagcccttgc ctttttttac tgttatagtc
     9361 atattattat tatctacaac ttgcaaattg ctacttggat tagggaatac accattacct
     9421 ataaaactag caaaatattc tctaaagtcc tctgccttgt atctcctatc gccatttaca
     9481 ctattaaata caaaactttt ttccattatt tcaccatcct ttttattttt gaaaataagg
     9541 ttggtatgct gctcccaaac gtaattttta atttcatccc atttttacta tagctttctt
     9601 gtacttctac aattctagta tccattgtta ctttcaattt tctatcttga actgtaacaa
     9661 tatcccctag tttgtagtct ttttcataca taaaagtatt tttagggtct atttctaatt
     9721 caaaactttt taactcttca tattctttta atttaatttc tccttctttt tgtagtatgc
     9781 ttatagtatt ttcacttaca tctgcaaaga cttcgtttct ctcaaaacct tcaacctcac
     9841 cagttgaaat taccagtttt gtcgcgtctt ctcttgctcc agcatacaca acatttttag
     9901 agttaattat actttctgta aagtgtctag ctcttatgtt attaaaatca cttctaaaaa
     9961 taaccggcgg gttttctgtt tgattaatag tcaaatcttt accagtaatt acgtcaaaaa
    10021 taaatttctt tttattagca tccaacacta cctcccaacc aagcccacaa aattctgata
    10081 tttcttgaat tttatcactt aaattttcgt aagaacttcg ccacatatca gcacttccaa
    10141 gatttttgtt ttctgaaatg ataagattat caattacacg ttttggattt gaagggtcta
    10201 cgcagttatt attaacaaaa gcttttataa tttcttcttg tgtcccttcg tgactttcga
    10261 aagcttctcc agtgtcgggt atgatgagcc ttctatttaa caagcctttt aaatccacgc
    10321 ctttaacaga taaaatgtcg cttttttctc ctgtttcggt gtacatgaac tctcgatgaa
    10381 gtatcaaaca aactttatta taatttttac caagtagaat tatattattt ttaactaatt
    10441 tatctacatg atttttatta gcatttattt ttaaactaaa ctctcctact ttgtggaatt
    10501 ttttaataat ctgtaattct tcgtaattat ctatttcagc tattaaattt atatttttat
    10561 ctagtattct tatgctctgc tgcatattac acccccaaat aaagtggatt ataatatata
    10621 gacatttcta aattatcaat tcctacgtct gcgtcatatc taaataaatt gtctccaact
    10681 tcaagttgta aaaattcgct atccaagtca atccaattaa atacatttat tctttcatag
    10741 tttgatttta ctaactctat tcttttattt gcaaattctg ttgttatttc taataagtca
    10801 ccagcttcaa gggttctgtt tatcttaata tattctcttg tgtttacatt aaataggcta
    10861 ggattatcaa ctgttgcaag tgctttaaat tgtattttca tgccacattc aacgtctccg
    10921 ttattcacta cattacaaat taaattactt accctatgac ccatttctat tccttcatta
    10981 cttatttcta aagtaaattc gaagtcacca acccataagg cgacttcttc ttttaaaaca
    11041 aactcatcca tccaaaatgg gcttggagtt aagagctgta taagaaactt ttgcatcaat
    11101 ctaactggtg tttgaaatgt tatgtcttgc acaactcctt tgacacgttt agttaatgcg
    11161 ttatttgtat atataaattc catttctcct aatttaggat taaacaaaga ggttaaatat
    11221 gcacgtttta attctaaatc ttctttattt tttgcataaa tagctgctga aataggaagt
    11281 aatctctctt ttatgcttat atcatctata cttacaccat cacctagatt gttcgagact
    11341 gaaatattag cactagtatt tgctccgttt tctatttttt ctaacaaaaa agggcgtgag
    11401 tttcctaatt taacttcttg ccctttcgag tttctacata ttaatttttg caagtttcac
    11461 accccttttt ttataaacta tttttttata aactaaatgc tagacttctt attgttcttt
    11521 cgatttctct tgctgtgttt gagtctgtac tattaacgtt tacattataa ttcgctgttc
    11581 ttgaattatt attataagtt gcttctcttc ttcccgaatt tgaattagct gctgaatttc
    11641 cttttctaat atcgctattt aaactcaatg cttcatttct agcattattt atttctcttg
    11701 taatactatc aattagattt ttaatttcgt ttatggcagg cttaaatcct tcaagtaatt
    11761 tttcacctag agtttgacct gcatttttat atgcatctga ataactattt aataacgcaa
    11821 ctatttcttt ttgattttta tccattatca atttttcagc ttctgcttga agttttgatt
    11881 cttttattct ttcatcatag aactttttag cgtcttcaag tcttttatta taatattcct
    11941 tttcgctttc atagatttga tttatatttt ctatttcgtc ttttttcttt tcttctaatt
    12001 cttcttttct cttttttaaa gcttcttttt tatcttctat ttcttgttgc tctattcttt
    12061 tttttctttc agctaaaacc ttctctagtt ctttttgcaa ctgttctttg ttagtatcat
    12121 catgttcaaa ctttatagct gttttaattt cttctatttt gtttaattct tctttgtctt
    12181 gttcaagtct atctttttcc ttttcagctt tttcaaaagc ttctaattcg ttgtcaatag
    12241 ctttaatttt cgcatcatag acactattaa ttctatctat actttcttcc ttccatttat
    12301 ctagtgcgtc taactctttt tttatactcg cttgctgtgc ttcgtattct tgtttgtatt
    12361 tttctttcag cgcatcaact attgcttttg ttaagtcatc aacttttttt acaaactctt
    12421 gtctgtcttg taactcttta ttcaactgtt ctttagctga atttgctatc tcttttagct
    12481 gttcaacttc tttttctttt tgttcttttc ttagtgctat cgcttctttt attttttctt
    12541 ttttagctgc atattcttct ttcaatgcgt ctttagcgtc accttttact tttttaagcg
    12601 ctgctttttc ttgtttagat aattcgtcta tttgtttatt aaatagtttt atatcttctt
    12661 cgcatgcttt tgtagctttc tttgtttctt cttctataat ctttgttatc ttttcagttg
    12721 cttttttgac cgagtcgcta ttattagcaa tcccttgtgc taaaccttca tcaacgaatt
    12781 taccaatctc agccataaca cgagatggag aatgtattcc taacactttt ctaaccgcat
    12841 cagcagcctt ttgccctagt tttttggctt cgtctactac tttataagca aagccggata
    12901 taccattcgc taagccgctt acaaaatctt taccaacttg caggaacttg cttgggaact
    12961 cttttatttt gtttacaaca tcagtcccga ctttttgaga tgcattaaca gcatcttgtt
    13021 tttttgagtc tataccttct cttagtcttg taaacatgtc gatagctgct tgttttaact
    13081 tggctggaag tgttttaaaa aatgtaatga cattattcca agtcgtcaca atgttatttt
    13141 taatttctcc tagttttgtt gttacagctg ttttcatgct ttcccacgca tttgtaagag
    13201 ttgactttat attttcccaa gtgttggagc aaattgtttt tagcccttcc caagcagtcg
    13261 aacatgtcga ctttatattt tcccatatat tgcttagagt ttgcgttata gactcccaaa
    13321 tggttattgc agtacttttc atactttccc acataccaga aagtaaatca cctattgcgg
    13381 ctaatgcatt gttaaatact tcttttatag attcccacgt attcgaaagt ccttgtttta
    13441 tattttccca aatttgcaaa gcgtctgttt ttaacttatc aaaatttcct gttacaacat
    13501 ctaaaattaa taaaaccgct ccaagcacca cattttttat aacttcccaa acattattaa
    13561 aaagctcttt tatactatca aaaataagtt ttaatttttc gataaagggt gcaaattttt
    13621 cttctaattg ttgttttata gcatcccaag cattactaaa aataagtttt atactttccc
    13681 aagttgtttg aaaaaataat tttatagact cccatttctc gcttatgcct tgttttattt
    13741 cttctatttt taagctgata ctttctttta tttctgtcca cttatttgta acagtaagct
    13801 ttatattatc ccataaaagg actgtatttt cttttaaatt agcccatcca atagatattt
    13861 tttcttttaa gccttctacc cagttccccg ctatgtcgtc ccattcttgc atcttacctt
    13921 ttaccgtatc atttaaaact tgccatttcg acttaatttc tcctgttgtc caatcaactt
    13981 tattcgcttg attttctgct tgtttctgtg cttctgcaac gactccttga tgcatttctt
    14041 ttgctttatt tattgattcg tctttttgtc ttgttgcttc ttctactact ttatccgcta
    14101 acttttcatt ttctgcgcca ccctctgttc taagctgtgc ggctattttt aatctttcgt
    14161 tatattcttc attagctgct tttatacttt cgtctttttt ctttattgag tttttaacta
    14221 cttccgcagc ttctttagct gacaaatcaa catgatttgc tttcattctt tccattattg
    14281 cagcttgctc tgcttcgctt ttactcattg tgtcaactgc ggtatttagc atatctgttt
    14341 ttatttgatt tatctctgct gattcttctt ttgttaatgt tctgtgttct tgactagctt
    14401 tagtcattat ctgatttact ttttcattgc tcttttttat cttttcttct ttgcttgaat
    14461 acgcttcttc agtatttttt attaactctt ctttttcttt gtcacttaaa tttttagaag
    14521 ttgcaaacat ttcttgcaaa gactgaatac cttgctcttt actctcttga agtttagcaa
    14581 tgatttgatt tgacatttct gtaaaattag atactattga ttgtttcatt ttctctgaaa
    14641 cagtctcacc actccacatt aattgattta aagatattgt tgtcttttcc tctaaatcca
    14701 taaaagcgcc tacagcttct tttgtagatt ctgatacact tttaccaaaa cggctaattg
    14761 gctctatagc atcctttgac atgtctttag cgactgttgc aaccgatatg cctagcgcag
    14821 taattccagc tatagctaca gctgcggggc ttgataatgc acctaaaatt gcagctaaag
    14881 caccaacagc tggagttgct gcggctgctc ctgtcgttac gactgccatt gctgctgaaa
    14941 aagtcccaaa agctcctacg accccaccaa tcataccaat tatttttcca ccaatcaata
    15001 aaactggtcc gagtgccgct gctaatcctg ctattttaac tatagtttct accgtgctag
    15061 gcttcatttc gtttaattta tttgtaagat tttgaaacca gctaaccgct tgcgctatgc
    15121 taggttttaa ggcatcatat atcttaagtc ccaattcctc aatagctgac attaaagcag
    15181 ttatagaacc cttgttattg ttttgcatag tcttagccat tttatctaac gcacctgtac
    15241 tattgccaac ttttttttca agttctgaat attcttctcc aactccggac aaaagagcct
    15301 gtaaagtaga tatttgtgtt tttccaccta tcatagatag atacattgtt cgttgttctt
    15361 ctgtcatgtc ttttgttttt tctttaagtt ccatcaagac attagccatg cctttaaatt
    15421 ttccgttaga gtcaaaagcg cttagtccta acttctccat cgctacgccg gcttgaccag
    15481 ctccactcgt caaatttatc ataattgaat ttaaagagtt tcctgcctcg ctgcctttta
    15541 gtcctctgtt tgcaagtatt ccaagcagtg cattagcttc cgaaagaggt acgtttaaat
    15601 ttttaaaagt acctccagac acaatcatag cttccataag tgcgtcaatg ttagtgtttg
    15661 aacttgcggc agttttagca acttggtcta agtaagttgg taactctttt acactaagtc
    15721 caagtgatga catagagtcg gttactaaat ccgaggttct tgccaaatca aggtttccag
    15781 cttccgacaa tcgcaaaaca ggcattaaag cctccattga tgtttttgca tcccatccag
    15841 ccaaagccat atatccaagc gcatccgctg cgtctttagc tgtttttgat gtagaagccc
    15901 ctgcttctct agctgctttt tcaagttgaa ccatttcgtc agctgttgcc ccactcaatc
    15961 cttgtacatt tgacatagat gcttcaaaat ccattccaag tttactagcc gctgctccga
    16021 ctgctagaat aggagcagta atactcatag tcattttttt acctacatct tgcatttgtt
    16081 ttccagccga gctgaattta ttacctattt cagtcatttt agttgccaac tgattccatt
    16141 tattttcctg ctgatttatt ctttctgttg tgtcatctag ttctgaacgc atttcagaaa
    16201 gtctcgactc tgcattatta actcgaatag accaattatc aaccgctgcc gagttagacc
    16261 ttattttttc ttcattttta gcatattctt gttctaaact atcaagttca gattttaatc
    16321 tttttgtttc ttctgcattt tcacctaacg tcgctttgct ctgttcgtaa gcactcttag
    16381 cttgattaac tttttcttta agttcaactt gcttttgtgc tgtgttgctt aaagttgttt
    16441 tactttcatt tagtttattt ttatatgctt caactacttt agtttgctgc tgtatagtac
    16501 tagataacat ttgttgctta gatttcaaac catcaagact tgttccaaaa tttttcactc
    16561 cagcgccaga atttttaaac tcgcttctta ttaacttcat ttgcctattt agattattaa
    16621 caccttgctg aaatgaacta ttttccatag cgacccgaac aactaattca cctacatctt
    16681 ccgccattct ctcacctcac ttgcaaaaaa ataaaccaag atttttatct tggtttattt
    16741 tatataaaac ttatattaaa ttaattttct tccgcaaaat ggacaatatt taatagtttc
    16801 cttactactg attcctaaat agtcgtaact tgctcctgtt agaaaatgct ttccgttttt
    16861 aagttctata tacatatcta tgtcggagtc gttagttaat aattcacctc ttacaactgt
    16921 atcatacatg ctaaacggtt ctgtattaat tttaacttca cagtacttac acatacactc
    16981 acatccttat aaccagctta tctggtcaat ataaacatca ttttcatttg gattattatt
    17041 atctttttta ttctctttca tatacacaag tatatctaaa taataaaata tatccatgtt
    17101 gtctatgtcc gtcatgctcc aatttttttc aaataaaaac atgctatata aatcttttat
    17161 aaaatcacgt ggagatagat acctattttc tttattagaa ttattttctg tatcttctcc
    17221 attacctagt tttttacttt ttcttcgaaa cctccactta cttttgataa atcttctaac
    17281 gctttattta taaaatcacc agctggataa aaaccatcta ataactcatc ttttgaaaat
    17341 tgttccccgt atatatcaac tgtaaaatca agcaattcgc ggaataattc tgtagagtta
    17401 agaccatctt cgattttatc ggttagtgta aaaactttat ctaattttct aagtgcaata
    17461 ggaggtgctt tgaatatttt ttcttcttca tttatagtta attttatttc catttttaat
    17521 tttctcctta tttatttaaa tttgctttcg ctggtgctgt tggttttact cctgttttta
    17581 aaaaatctgt tgtaaaaaat tgcgcattag taggtgcatt ttcgacgtcc gtatctgcgg
    17641 ttattttatg tctaccatca tgtattcttg gcataaattt aagtttaatc ttttgtgatt
    17701 gaaattcgat tttatcctct tttgtttttg cttcttcttc gaaaggttca cacacgcctt
    17761 tagttagcca aaccattcta ctcccgtttt gactttttgg agattcaaaa ccaaaagcaa
    17821 gattcggagg cgcgaaattt ttatcttcta tcagtacacc atctttatat tggtatccaa
    17881 ggataagcgc acgttcttct aatgttaagt cagcgacctc aatttccacc tctatagctc
    17941 ccatagccga agcagtctct aaaagctggt catccccata attttcagca gaatcaacct
    18001 taggtgatat ttttacactt ttagcacctg ttaatcttgt tattttagta tcgtattcaa
    18061 gtgtttcctc tgtatctgtt agtagcttta caattctata gtttcttaat cctataatag
    18121 ccatttctta atcctccttt ttttctgcaa aaaataagaa tctaaaaata gtatggtaac
    18181 tataatcaga ttcttttatt tcgtgtatac ttctttttat aaaattattt tttttaagaa
    18241 gtctttcaat ttctttattt atataactca ttttattctt actaaacaaa tctatttgta
    18301 tataatgttc tgtgcctact acttcatcat cagcatagcg gttttgttct aaataactaa
    18361 aaaaagttat atatgttttt tcttctccat caaacctttg aaatgaaaca ggaattttta
    18421 aaggttttaa tgtttctatt actaaatcat ttatattaat cattataacc ccaaccctct
    18481 ttttaatatt tctttcatta catttttagc ttcatctttt tttgattcgt atgcgggtgc
    18541 tagaaacggt ttagaactgg ttcctggatg atttactacc cttccttttt tcttaccttt
    18601 tttaattggt attttatgcg cagatgctcc aaactctaaa aactttccat aaaaaatttc
    18661 tgaattatcc tctttcgtaa taccaactaa aacatactta gtaccacctt ttttcttaac
    18721 tccacttact tttaaaccat cttttaactt tccgcttctt ttgcttaccc ttggtaatgc
    18781 atcttgcaaa ataacattac cgccagcttt tacagcttca tttaacagtt tatcgcctgt
    18841 tttacccatg ctttctattt tagataaaat agcgtcaaat ccttctgttg ttatctccac
    18901 actcatttaa acaacttcct catatttaaa ttttaattct ttacaataat attcattttc
    18961 tataaaatca ctagaattta aaaagataaa attttctttt ttaaatatat cttttatact
    19021 aatatatttg tttatattct ttaagctctt ataccaatat tctattttta ttgaatatac
    19081 aatactttta ttttcattat cagaaaaatt tgcatcatat tcattttcta taaaaaatat
    19141 tatatattct tcatcatttg aaaaattgtc gtattcttga aaaccaatac ctagatttaa
    19201 ttttttaagt atagtaacta tttttgagtg catactatct cctaactttt ttttacttag
    19261 attctgctgt tatttcaagt attttatttt cttcttctaa atttaagatt tgagttatat
    19321 tataaaattt atctttgtat ttaataacaa aattagttat agcattttgt tttatactta
    19381 aatctaattc ttcaatatat ctaattttaa actttttatt tgtagaagtt ttaatcgaat
    19441 tattttctat caattcttta ctatttaaat cttcaacaca agcccaaatt tttttataaa
    19501 attttattgt tacatcctca agaacatttt ttctctcttt gataaaaata gaaatatgtt
    19561 tatctaatac gtcaactaat tcagttaagt ataaatacac actagttttg tcattgctaa
    19621 tatcaagttt tactatgctg taaaattcat tatttaactt tataacatca cttgttttaa
    19681 ttaatctcat gtaaggaact tttaatttaa tagatacctt taaccctgta tcgtcaaatt
    19741 taagtttgtc ttgctctctt atatattcat tagaataaaa gagtttacca cttacaataa
    19801 actctttttc atctaacgca ttaccttcta tatcataact ttccttgtat tctccaaact
    19861 caacaatacc atcattaaaa ttattaaatt ttattttttt catatctata ttacctcatt
    19921 agctgcactt ttaatttgaa gtctaagaag ctctttacta aaattttcct cgaaatattc
    19981 aatagcattg tttcttacat atctgcaata attaaatagc aattctctat taaaattatc
    20041 tttaaaatcc agtgctattc ctgctatttc attttcaaag taatcaattc ctcttacaac
    20101 cattttttct atttttgcat cttcttctat ccaagttata tttagatatt cttttacctc
    20161 ttcaactaca cttttaatgt tttcattttc catatatttc acctatttcg ctctagcagc
    20221 tgacgttttt gtgtttgaat taatttctgt tgctaaagtt gtgtctaagt tttctatatc
    20281 aaatactaaa aatgaatcat tgtcttttgg cgctcctgtt gcatattgtt ttactagata
    20341 agttctttcg tcctctaaaa atttatattc gtctgaatac tctatttttc cgcttgaagc
    20401 aactcccata aaataatctt ttgccatacc agctattaat ttacctttag ggacagcaac
    20461 tgattgaatt atattcccag ggataggtaa aactccatac acataagtac cattagatgt
    20521 taacatagtt gtggcagcga aaattttagc ccagtaatca accggattta caagcattat
    20581 aacgcttggg actgcccttt taccattgtt tgtaagtggt gccataatct ctttgccaag
    20641 tgttattggt ttaaaatcag ccagtggttt tgcagttttg tctggaaata cgccttcaac
    20701 gactgcgcct tttaaatctt ttatcatacc tttcggctgg tcttttcctg ttcctgctac
    20761 tattcccatt tcaagcccca atgatataga ttcagtcaaa actactctta catatctgtc
    20821 taaccattca gcccctaaat caagcatggc ttttgcaact ggaagataag cagataattt
    20881 ataagcttct gtattaattt tatcaaatcc atgctctagc tcttttttga tagtatcatc
    20941 taatctaccc caccatgcag cttctgcgtt cgttttcctt attatccact ccgtaactcc
    21001 tgttgtattt tcaaaagtta tggcactaag caaagggtga ttttgttcta agtcgtcaaa
    21061 tactctatca aatacagtac gtggcatagt tatctctaag ttggtaaatc ctcttttttc
    21121 tataacttta ctatagtatg ctctttcctc gctagttaat tgcggcatcc ctcttttgtc
    21181 aagtattgct ctgtcagctg attctatatt agcaatagaa cgcgcttctt ttagtagatt
    21241 ttcttgtata tcttccgcca tcctcaaaaa cgcagttgtt attttttcct cgtcattacc
    21301 ctttattgct tccattaaat ctgctcttag ttcaacatta gttgcttcat ctaaattttt
    21361 aattgccatt tttattttcc tcctcgatta aatgcattaa aaaaagccga tactaaatcg
    21421 acatttcttt tttctatgtt atctttatta tcattttgct ttgtttcttc tttttgcgat
    21481 tctctaagct cctttataga tatatcactt ctacaattta tttctgtgtc attgtaagct
    21541 ggtatcgggg ttgcagtaat ttcaaacaat tcaacttccg taatatctct gtaaaatgtc
    21601 caatcatcat cccatcgagt tttagaattt acaatattaa aaccaaaaga acatccttta
    21661 atcaatccaa gccttacatt ttcaagtaag tcattaccat ctgttgtagc tggtatatct
    21721 acttcaaacc ttaacccatg ttcatcttct tctaaaatta gatttgagtt agttctacct
    21781 acaactttgt tccaatcatg atttatgagc ataaacttat ctctagtgtt atctgctaaa
    21841 gtttttaaaa atgcacctct tgaaattttt tcataaaagg tatcgcccca tctatcttgt
    21901 aacttagtgt aatcgtcgct aaaaactgct gcataaccac ttatcttacg gctttctaaa
    21961 ttactatccc ttatttccag cgacaccgtt cttttctcca tttccattgt tagcccctcc
    22021 tttcaaatct ggatttaaaa ttgactgata gtttttagtt acatagtgtt catcagccca
    22081 agactcgttt attttttctc tgccaagcat ttctaaattg tcatttatag aatttacacc
    22141 tattctaaat aataggtcgc ttgctttcga tattttttca agtcccatat tagcaatttt
    22201 ttgtgtatct acttttacat aactcttttc aaaaaaatta tctctaccat acatttttct
    22261 agttaattca tttgtaataa gttttgctac tggattgaca gaaaaattca agaaattatc
    22321 tgttatacct tcaactccag caacattacc ttttacaagt ccagcaggaa catgaaaagc
    22381 agcgcataca aaatccatga cgtcatcaat aatcgctctt atatccctgc tatcttttat
    22441 acttgaagct tttgtatttt ctgtatattt taatccattt gaaagcggta aaacagcatt
    22501 atcactttca aaataacgtt taaacttatt actcatcaaa tcgttaagtc gttcctgcgc
    22561 ttcttttgtc tgcgggtaat ttgtgtctat ttcaagtacg cccttttcgc tattattttt
    22621 tttataaccc ttcatggcac tacttaataa atctttataa tcattataaa gataatctat
    22681 tacagttttt atactttcaa catttaattt gaaatataaa acatctgact ctctaaaact
    22741 tctatctaat ttatagtttc taattgtaac atctttgtat atatcttcat aaaaaacata
    22801 cttatctaca ctaaaacttt cagctacgaa aagttgttcg ttttcttgta taactagaca
    22861 ttcattgtca tacacaagtc ttgtaattac ttcttgccaa aattctgttg cgttttggtt
    22921 ttggttaggt tctatattaa acaagtaata atttttttct ttaatagatt ttccattttt
    22981 aaatgtctga aattcactta aaactaaagc atttgcaata atagaaatac atgactttaa
    23041 cgcaaattct ttataaaata tcgtagcttc tagtttttta aaatattctc catctgctcc
    23101 tgacatttta tctttaaata aaaaattact tataaaattt ttaaaattca attcaccacc
    23161 tcattattaa tacgtataac aatcataaaa cttaattgtt gattcttgca tcagttcatc
    23221 atcttttgat aaagcatgta aaaacgcaaa aaagccatca gttttccgct tgattggctc
    23281 tatctttaaa aaagttttat ttcctttttt gtctgtatct acataaacat tattagtata
    23341 ccaacgcatc atcatgtcgt cgccccactt aattgtcctc tctgcgaaga tttgttctac
    23401 aagaggggct atttttgcgt gagttatcga cccatttcga acttcttcaa tcggtaaacc
    23461 tttgttagta aactcttctt ttacatgtgc agctctataa gagtcacaac agatttttaa
    23521 tatattataa tcttctgctt tttcaatgaa ccaatcagca atatattccg cttttatcgt
    23581 ttcatcataa attattgtgc agagaccttt ttgctttgct aattcaatat caaatttaaa
    23641 atttgttatt tctaaagact tataacatat aaaagtgtga tgcatgatat atcgtttttt
    23701 atctttttta aataacaata ccacgcttgt aaaatctctt atactagaaa aatctaatcc
    23761 tccaatgcac gaaaagcctt ttaagtctgg taattcttcg tttgttgcaa ctatgtcatc
    23821 ccattcagcg acaacagttg ccaaatcttc agctggcaaa ttaaatcttt tagttagtag
    23881 ctctatcatc atttgtggtc tatttttagc tttgtcaatg tcttttaaaa tttgtgtcct
    23941 taagtctgaa ttatacttta tacttgggtt tgccttttct agcaaattaa catcttccat
    24001 ttcttctata ctatcaagtt taaaaataag tggcagcatt ttagagtttt taatttcttc
    24061 atttaaaatt tgtttagctt cttctttgaa atcgtctaat actccacctc ttacattccc
    24121 atcagttgta atataaaaaa ttctaggatt ttttactttt ccaagcgcag aggtaaagac
    24181 ttttatatta tcataatttt cgtatgcatg aacctcatca aaaataatgc agccgggtct
    24241 taatccatcc tttgacttag cattatttgt gcggaacttt aaatagctgt tagttttttt
    24301 aaatgttatt acttctttag ttctgttaaa aaacttttta catatagcag gatgttcaga
    24361 taacatgtta taaacatcat caaaacttgt ttttgcttgt tcctctgaca ttgcaacaat
    24421 gtcaatacca taatttttta caccatgcaa gtgcgtttgt aaataattag ctaattggga
    24481 tataaaacca tttttcccat ttcccctacc catcatgata aaaatttcat catataaaag
    24541 ttcgtcgcct tcaaagcagc caaccactaa agcaacaaca aatttttccc aagcatacat
    24601 tttatagaaa tacttttcca tatattctat agctttttca actttttcat ggtcgatata
    24661 gatattaggt ttttttaaat ctttttttat tttcttaact gcaagtttca tttctttaca
    24721 tagaacaatt tcgcctttat cagccaagct gaaatactca tctatgtatt tattgtactt
    24781 ataattcaat atcatcatca cccaaaactt ttggcgtcgc ttttatacct agtttatcaa
    24841 gtaaacttaa catttgagcg ttatacttga taagctctga tacactatcg ttttttttat
    24901 agccccattg attttgacca tttttgtatt taacttgaac acctctagtt tttacatctt
    24961 ctattaattc attttttata tcccacattt ttaaatagtc ttcaactaag tctttgaagt
    25021 attcttctgt catgttattc tcttttattt gtcttaataa atcttttctt atagagtttt
    25081 ttaaattatt tcccgactct tgcaacccat ttttcacgca accctttttt ggggtgcagt
    25141 tgcgagtcca accccctctt tttctccaac ttttcaaagt gttttcgggt atgttatact
    25201 tggctgacat ttctttataa gtcataccta aaacgtagtc attctgtacg tctcgcctaa
    25261 tttctaattc ctcattatca tccattttca ccacctcatt tttatgtttt ttctgcaccc
    25321 gcaaccatga attttggttg caagaccccc accccccaca ccttttgtta aattggtgtc
    25381 tccttgcgga gccatgtacc tacctcggtt cacaaaattt cgagctgaaa gccagttttt
    25441 tcaatagggg ggctatctac cagcgttctt cactaaaaaa ttttttcttt ttctcttgat
    25501 taatttttag tattttttcg tgaatttcgt tgtgacaaag gttgcacaag cttgttaggt
    25561 tattaagctt caaagcttcg ctaggatact cttttatttc tttaatatga tgcacacatt
    25621 cggctttaga tactttcttt ttttctttac aaatctgaca ttcattatta tctctttcaa
    25681 gtgctttttt tcttaatttg cgccagtctg tacttctata gaatatatca agtctatcaa
    25741 ttaaaattaa ttcttttaat ctatctagtg tcatgtgctt ataactcatt aatatacaac
    25801 tctcctagac cgtcgctata ctctttcatg tcttgcaata gcttgacttc atcatcttta
    25861 agcttcttat attcttctct cttatgttta atataatcat cctcatatga tacaacaaac
    25921 ttctcaaagc atctgcgaca catgaagtat gtaacagtag tattatcaat aactttagtc
    25981 tttaaatctc tattatttac attaactttc tttccgcaat tattacattt aaacttacca
    26041 tacttattca tagtgatact cctttaattt cctagttgag tttaacataa ggtatattct
    26101 gttaaactca tttaagtatt atcaaacagc cttacacttt agtattaaca atacattcaa
    26161 gactttatta ttagactata ttttatccag tttaacgtga cctttttatg ttaaactctt
    26221 aagctaaaag tatttaatat tcctatatgc tttgtttaaa tcatcctgtt caattccaat
    26281 ataccgcaag gttatcgtag ctgaactatg attaaaaagc ttctgcaatg ttacaacatc
    26341 atgtgttctt ttatagtaat gataaccaaa agttttacgc attgtatgtg tgcctagatt
    26401 atctatacca aacatttcgc ctacttctcg tattatctta taagccatga ctctgcttat
    26461 agctttattg gtctgctctc tactcttaat taaaaattca ttttgatttt tatcttttgt
    26521 atattctttt agtattcttc tcaaaatagg attaacctcg ataggcttag tcttacccgt
    26581 cttactttct ttaagagtta taaaccgttt gtcatgaaca tcttttattc taagctttaa
    26641 tatgtcccct actctaagtc ctgtatatat gcccatactg aagagtacat tgtttctcat
    26701 gttagtttct tctaaataag cgcatatgct ttctagcttc tctaaatctc tgataggttc
    26761 tacaaaattc atttactcac ccccttcctt tttttagcaa taaaaaagcc taaactttcg
    26821 agcttcaagt ttagacttta ggtttcgggg gatattcagt tgtcaatgta cacttttttc
    26881 tatgatacaa ttctaacatg ttttctttag aagcgaagta taaaaatagt cttttttctg
    26941 taaaaataat gtttattcta aagcttcatc accatatatc attaaagcta ttttattaat
    27001 agcaatagct tttttgttat aaagactagt tctatcataa tttaattccc ttgccatttt
    27061 tgtaataggc attttcccat cccagctata aaagtatact agttctataa ttttttgttc
    27121 tatctctgat agctctttta aataaatatt ataaaactct agctttcgct ctataatttc
    27181 aatttcagtt tctttgttgt agatagcatc ctctattgtt acaaccatgt catctatacc
    27241 ttttacactt ttttgtactt taaaccctaa ttcttcataa ttaacttctc tatagttttc
    27301 tttgtgttta agaattgcta tttctttttt taatactctt aactttctat atttatctcc
    27361 tagcattttt aatgcatttt ccactttgtc aaaatactct ttttttattt tcatttttac
    27421 acctcttgca ctttttgtta taaataccta actaatcctt aaatttagct tttgggacta
    27481 gttaggtatt ttgtagcatt tccaacccgt ctatattatt caaaatattc tgtattttta
    27541 ttttgttttt ttgttataag acttcatttt tttaagttta aataaaatct atatttataa
    27601 agcattttat ctattttaat ttaaaaatta atctaattgc gttttatatt gcctatttac
    27661 tttaatttat ttttaaataa ctttttttca tttccaattg gttttgcaca ttgagaacaa
    27721 ttccccttga acatttctga acagctagga cacattttcc caactggagt ttttatagaa
    27781 aaaaattctt gaccacataa aatacattcg cctactacta gttcatctaa atagtctccg
    27841 cacaaacatt ttctacacat actcatttta actcccccct atgctaattt attttttcga
    27901 gtttccattt tctttttctt agttttttat tatgtagctt caaaccttcg cgtatttctt
    27961 tatcaatctt taatacttta aatagtgtaa aaattgcttg aataacatcc aaaccttctt
    28021 ccattatttt tttgttatct tttaattcaa tagcttcttt aagttctaaa acttcttcaa
    28081 gtactttttc aaattgttct tttgctgtca attctggtat atttgcaatc attttaaaat
    28141 tcatctttta ttttctcctt tttatatttc ttctaaaatt tcttcatgtt gtttttttat
    28201 tgcatcaaga ccttcaagta tcatttttct tataatttct ttcatgtatt catcatgtgt
    28261 aaatttaaca atttctccaa ttttattttt cttgtcaata aataaaattc taacttctgt
    28321 ttctccgatt tcaatttctt ttaaaatatt ctctaagtct tttatacttt cttttgttgt
    28381 gcattgtatc tcctctttaa tcttagttaa aaattcttcg attgttgttt ttcccatttt
    28441 atttttctcc tttttttatt cttctgcagc tttcaactgt aaagtcacta tctcttcttt
    28501 taccttctat aaaatttaat tgtttgccac catttttttc tatatattca gatatttcac
    28561 ttttgtcttg gcattcattt tctacatatt caagtatgtc aagtatgcac atttcttctg
    28621 gaacttcaat aataacttta tgttttaaaa atatcgtttc ttcaatttca attctatact
    28681 ctttcatttt ttctcccctt cttttctgta ttaaaaaaat ttatctgtct ttcattttct
    28741 atttcttcat cactaaatcg tttttccaag tcatgcactg tagttccatc ggctttgaaa
    28801 gaaacaggac tatccttatc caatttaagc atacccccca caaatctgga taatatcttc
    28861 ttagagttct aaggctctct aaactttgtt taggacataa gtaacaacca gttcgtttaa
    28921 atctatgatg tattttataa tagaaccctt tttcttctaa atacttcaag caatcttttt
    28981 cagtcatttt tgcttcgtaa agtggtgcta tacagttttc ttccagtctt ttatatctat
    29041 taggttcatc aaaggcaatt ccaatgtacc ttttatgttc tccaaccgaa ttgaaatatt
    29101 tattagcagg agcaagtttt aatcgactat tacaccatgc tcccaaggtg tatggaaaac
    29161 cccatatttg ccctttacgc ttaccttgtt tattaacagt ataaaagtat tcctcaaagg
    29221 ttttttctgc tttaattctt gttattttaa agtttattct tttttcaaaa tcatctatta
    29281 tgttataaat ttcttcaaat tctagtcctg tatctataaa tacgatttca tcaagagtta
    29341 atttcttttc taatattaga aggagcattg ctgctgaatc ttttcctcca ctaaaacttg
    29401 caatatactt cattttttcg ccccttctgt ttctaaattt ttttctcttt ttttcttaca
    29461 tttcttagaa caatatgcaa ttttaccatc cgtataagaa taaatcgttg ttccacacca
    29521 tccgcaaatt ttagctgttt ttttatttat attagacatt attatccctc caactttaaa
    29581 atggtatatc cgggtcatct atagcttgaa atccctgttg gtctaaacct ttcggttcaa
    29641 acatatattc tttttcatta tcctcttttt tatagtctaa aaatttaatc ttattagcat
    29701 gaacttttgt ataagttctc ttctcgcctt ctttttcata actattaact cttatagacc
    29761 cttcgacccc tattagccta cctttagtca gattattagc acatacttct gctaacttcc
    29821 ccatcacctc aataggtata aaatcagttt ccttagttcc atcttttttg atataatctc
    29881 tatctattgc tattgtaaaa gtagatacag ctgttccaga acctggtata tatttcaatt
    29941 cagggtcttt tgttagtctt ccaatcattg caacattatt cattttaaac cacctttcac
    30001 ttttttatat ttcttcaatt ccaaaatggc gcttcttctc catttctttc atcatttaaa
    30061 acttcaacta tcttataatt ttgtaaacta tcttttcttt caagtgactt tatccagtcg
    30121 taaaatttac ctctatattc ttttttataa acatgattcg gaaggtcgtt ccaattatct
    30181 agtaaactat caaatttctc taatgcttgt gtaacactaa tcttattcat tttctccctc
    30241 cacttgttct tcaattattt tatctacgca gcctaaacag aaatacaacg caaaacttaa
    30301 atttaaagtt tcttcttcat acttcacata attattttct tctacattta ttgttctttc
    30361 gcatctagcg catgttacga acttatccat tattaaacct ctccttatat ttcttccatt
    30421 tttcgaacat ttttttcttt tagttctata aaaaaatttt ttaatagtat tttttcttct
    30481 tctcctactg aaaaatgaac gcaagataca tcacaagaac ctcgtatgtt aaactccata
    30541 cttaattctc caattccaaa ataagaatca atatttctta tcattgtatc aatgcctttt
    30601 cttctatcct ctaaaacctc gtatacctta attttttctt ttgtttcttt gtattgttca
    30661 tacagtaatt cctgctgctc gcataatttt tcatatttat ttatgttcat ttttgcgctc
    30721 ctttttaaat cttttatttc tacaaggaaa ttatttgctt tattttttca attttattag
    30781 atatagaatt aatattttta tttttaattc taatactttt agtcctgtta taaatcgcaa
    30841 tagctttcct tatatttttg tttaaaatac aatctatgaa taaattataa gtttctaatt
    30901 ctaaagcctg ttctattaaa atattttcaa tatctattat gcattcttct gactctaacg
    30961 catcccttgc aaaattttct aaactctttt ctaaaccttt tatatgtttt tcacattctt
    31021 ttaaatattc ttctttttgt ttatttcctt cttccaactc ttcaattagt tgtttcttaa
    31081 ctaatgtata actacctata ttcaaaattt tccctcctgt tcttctattt ttttaacttc
    31141 attacaagca atagaaaata attctccttt acttagattt ttatattttt cttgtagttt
    31201 cttttgtttc ttactaatca aatcagcttt ttctaatata ctcatctctg aaattttctt
    31261 ttgcatttac tttgctcctt cttgttctgc tacagtaaaa tttattatcg tctcatttcc
    31321 cctgtgctat actaaagcaa aaggaggcga tactatggtt actaaccaag attgtataag
    31381 agatatttta ttatttattg aatctaatac aactcccatt tctcctgtat tacaatttga
    31441 tactatacta gataatctca aatatgatga atcaactatt attcatcata taaatttaat
    31501 ttatcaaaac agtctcgttg atgatgttaa ttatggtgat gatactgttt tagaggtcac
    31561 gcagttgtct ttaaaaggct atcaatattt agatgaaata cgagacagca ctaaatggaa
    31621 taaaataaaa agttccacct caagtttttt aaatatgtct ctacctatcg caatagagta
    31681 catattaggt aagttttaat agttttaaat ataaggttta gattatagat atttctaagc
    31741 cttatcctag tatttttcta atacgttctt cttcttttag tagttcactg atagtgtgtg
    31801 tttttataaa accttctgaa aattggtgct gacctttttc aaatttttta attagtacac
    31861 tttttttgct gtcatctctt tctattgctc ttaaaaatga ttcataaaat atatgctcct
    31921 tgccaaattc ttttaaaata tattccttgg tagaatcatt atagccatgc tcttttaata
    31981 cttttatttt ttcttcataa cttaaaacat tattttttat cattttttca ctctcctttt
    32041 tcacatgtaa agatactaat ttaaatattt atttcttcat tttcttaaac tgctttcttc
    32101 ttataatact ccacgaatta ccatccaaac atttctcaca aatttcaata ttttttattt
    32161 cttttgctga actcaaataa tctcgtgtta aaaaaggtat tttaaataca tcttcttgat
    32221 tttctatttt tttagaacaa ataaaacaag tacattctga atcactagta tctttacctt
    32281 ttgaaacact gtcatgattg caagatattg tatccgtttt tgctgaacta atttcttcgc
    32341 aacttgcaac agtttcagaa ttttctttat ctccataatg aattttataa atttcttcta
    32401 tatctgcatt ttctaatcct agataagttt tatatgcctt ttcttttcta tcactgtgct
    32461 tatgcttttt tattatatct aaagcattta tttcatataa tagagcatca ttccaaggtt
    32521 ttttaaattt gttttcattt gcttttatta gttcttcgtt acttaactcg tttacagttt
    32581 cattacaaga aacaacattg ttactctctg cttttataac tccaaaaagt tcaaccaatt
    32641 cttttaactc tcttacacta ttaatttcca tttcttacac catccctatt tttttattta
    32701 aatttaacac tttgactttt ctttataatt gcatcaagtt ctttttctga atatttatta
    32761 aatgtttggt caaaattagc aaatttatta ttatttgtct ttgtttttcc tttgacgctc
    32821 tcattgccct ttagagcctt tttaggtgtc tcgtttaagt aagactcgaa tttgtttccg
    32881 aaaagcgtct ccgggcgtaa ataagcgttc attttatcat catctagcca gtttaacact
    32941 ttgttatcta tcaccttgta aaattcttct tctgtaaagc cttctttgag tcttgcatca
    33001 atcaaagata tagttttctt tgttgtactc ttaaaagatt tatctgctgt tttatttaag
    33061 taatcaacga ttcttttata tatctctttg tcttttgtct tttcttcttt ttgctctgca
    33121 gaactatata tattattagt taaattagtc ttgttaatat tagtcttgtt actcagcacc
    33181 tcattcctaa catgtacagc accacatgcc gtagggttaa gcacctcatt cctaacgtta
    33241 ggcattgtgt gctgcacctc ttgtttagct gataataata tatatagatt gctttctttt
    33301 tcattactac cttttcgctt gcggtttttt cttataagta accctttttc ttctaaactt
    33361 ttcaaaacat ttgatacttg cctttcgctg catccggctt ttttagatat tgtcttatag
    33421 cttggaaaac aacaactttc ttcatcgcta tatctaatta aaactatata aatcatcttt
    33481 tcgtatatgc ttaaatcttc tctatcaact aaactatttt ctgcccaaaa ccaatctctt
    33541 ttccttgaat cttttaaaag ttccaatccc tcacccccct tccacttata aattatttat
    33601 taagatagta atttaagttc atagtcattg aatatcaaat cttctttagt taatactttg
    33661 catgtcattt cttttatttc ttcgtcataa tacgttaata ctatttcgtc tttatctata
    33721 tatgtaacaa tacattttct atcatgatta aatttcacat catgcaatct aataatttcg
    33781 cctattttta tatttttact ttttagtaat ttcaatctat ccacctctta atttcgaata
    33841 ttctgattct ttcatatata ttttttactt ttttcttctt caatttcaaa aagatattcg
    33901 gtacttaaat cagggaagaa tattgtttgt attttttttg cctcaatata ttttagtcta
    33961 ttaatactat ttaacttggc gcaaattgta cttacattta aatttaacgc ttttgctatt
    34021 ttttcatttg ttatattttt tctcgccatt tctgctttta aatttctaaa cattttaatg
    34081 ctcctttctg aatttattca cgaaattgcg tgtcttatat ttcatataat acactcaatt
    34141 tcgtgaatag tcaatatgtt tttgtatttt atttttgcaa tttcgtaaat ttttcttgaa
    34201 tttttacgat tttaccatta tattaaaaac aaggtggtga ttaaatgaat atagaagaac
    34261 gtttaaaaca attaatactt agcaaatata aaagcataag agcttttaca aaagaagtta
    34321 atattcctta ttctacagtt gatactatgc ttaagcgcgg aataagtggt actagtataa
    34381 caacagtatt aaaaatttgt aacgctttaa atattgacgc tgatgctctt attgacaacg
    34441 aactaaaaga aaaagaacta tcttcaaaga tacaaacaaa tcagttcgaa caaaaaattt
    34501 taaaaatgta taatgctctc gatgaacatg gaaaagatat tgtatttact atattagaaa
    34561 aagaatatgt tagaattaat aagctctagt actagagctt atattttgtt ctacactcta
    34621 atattaacac ggaaaaccat tccaaaaata ttcgtacgac gaatattccg actttaattt
    34681 acaaaattta ccaattaatt tgtctatttt ctaaattgta aaaaatttct attgatttta
    34741 atatttttta atactatagt ttaatttcga catggagtct ttattaaaat atcatagaaa
    34801 aaagaaaaaa ctcactcaac gtcgattaag tgaattgaca ggaataagtc aaagttacat
    34861 ttcaaggatg gaaaacaaaa aatttaaaca cagcccaacc ttaactcaaa taattttatt
    34921 agctgatata ttagaaattg accaattgat tttatctgaa ttttttttgg aaaaagaaag
    34981 ggagcattta aaaaatacta aaaaatataa taaaaaagca gttaaaacac ttagcaggga
    35041 ttttttaaaa gaatataaaa ataaggacta ggtaattatt tatacctagc cccttttttt
    35101 actgctctac tttaatttct cctagctcaa tccagttatc tttatataat tccggaaatt
    35161 tcttttctac atctgatttt gatgctcctt gatttgcgta ctccattgct tcaaaaagtc
    35221 ttgaataatc tttcaggtta tattttaaaa ctatttcttt tacattcata ttcttttcaa
    35281 ctaatatttt aacttgtgca tcaaatttgt agttagctgg tacttgttga ttgtctaatt
    35341 tatcagttac ttttatgtct ttaaattcgt aaccatctgt accgactgcg ttccatcctg
    35401 tttgaaataa tttaactgct ttatctgttt tattttctac ttctacattt acaacttcgt
    35461 acacataatc agctgccttt ttcttttcga ttccatcact aaaatattct ccattttcgt
    35521 taaaacttcc gtcttcgttt atatctcctt ttatatcttc ttttgtaaca tcttttacag
    35581 ttattttaat tccatcaacc tcattttcat ttgctttctt ttctttttta gttgtgtttc
    35641 cagtatcttt gttattagtt gatgaacaag caaccaaccc tacacttaat aaacatactg
    35701 taattaataa taatactttt ttcatcctcg attcctccat gtttttatgt tttgttatat
    35761 atttcaacat caaaacttat attcctttaa ttttctattc tagtagtatg aaaataattt
    35821 aaaagaataa tacctattta tttccattct aagcattttt ttatgctatt taacataaat
    35881 tatttataac aaacagaaaa atcgtttctg gtgtcttgta tcgcttctaa atgatattct
    35941 agtttcttaa tcaaataatc attttctttt tatccaaaga tacattctaa tagtttcttt
    36001 acttttgaaa aatgctaata atattttaaa tagcgaccac cactttttga gacaagttag
    36061 atttctaatt aagttaagta ctttaataaa taaatacaaa agatacatat tttgttctct
    36121 ataatacaag ttctctataa taatatattc tctatatatt tactgcacca ctttttgcac
    36181 taccaaagca ccactttttg cactaccaaa gcaccacttt ttgcactacc aaagcaccac
    36241 tttttgcacg tagtaagaaa tctaattaat ttttaaaaac ttattaacat gttatcaaca
    36301 taaaaataat ggttatcaac ataaaaatca aagttataaa cagcattgtt atatttagac
    36361 ataaaaatag acctttttta aagaaggtct attttttacg tgcatttttc cattctgaaa
    36421 aactacatag tctatataat ttttgtatcc taggaacttt tttattcttt tcttcgtccc
    36481 actcaaattt attttctttt tttacttcga tatatttgct tacttcaaat actgttataa
    36541 tttttcttat tttacttctg ttatttttgc tatcaccaca taacccgatt ttttcacaaa
    36601 taaatttttc agtcatacat ttaaaactat tttcagttgt catgtaacat aaaaggcaat
    36661 aaacttttat tgcattagta ttaaatgcag aaattaattc ttgtagcata tcatgatgaa
    36721 ttgtaacaaa tttatgaacg ttatcgttgt atcctgtact tgtaccatag tttaatctat
    36781 aaattattcc gttgcggctg ttttctattt caagtacttt acaatctaaa ctattgagct
    36841 tttttatatt tctctctaaa gtagtacgag caacccctat ttgtttagct aattcttcca
    36901 ttttaaattt attttgatac aaatatctat ttttttctat atgcataatt ctcatgctcc
    36961 ctttacttat aagcattaaa gcgccaaact ctttagcaca ataatttttt tcatttaaac
    37021 aatacgtagg cattggtaat ttataatttt tttctaattc ttttgcttct tctaaagaca
    37081 ttttatttcc cccttttgac ggggcttgaa gcacttttaa ctaattaagt taaaaatttc
    37141 aaattatttt taaccttaaa acattgattt ataagttgtt ttaatctata atataactta
    37201 taaattagtt aaaaaaggat ataattatat cctataagag cttcaagctc cgttggtaag
    37261 gaataagtcg agtcgccaaa caaacgctta ttccttttat ttgtttttgt aatctgtaat
    37321 aagttaatat taattatagc ataaatataa aataaaaaaa tatattgatt ttgaaaataa
    37381 attatatttt aaaagactta gtacacttac taagtctttt tttatgtaaa tatatgttat
    37441 aataatttta gcgaggaaaa aagtgattga aaaagcgtaa gagtggctat ttccatattt
    37501 aaacgccaaa ttccataacg gaaggaggtg gaacaatatg gtgataaatt ttttattgag
    37561 tatactagct ggtgttatat cagcttccat atatgataaa ataaaaaacc actcaaacgc
    37621 caataagagt ggttcaagaa aataagttat ttttcaaaac attggaaata gctactcttg
    37681 tgtaaagtaa atcattattt ccttgctttt attatatcac attttttaaa aaagatacaa
    37741 ttttacatca cttcatatat gttaattagt ttaaaaatac aaaaccactc tgcgcaaaag
    37801 agtggttggg aatttgattt aaaaatcaaa ttcaaaaagt ctagataatt ttttacttta
    37861 gacgaaaact ctactcttat gactgaaata cagttctctt gcttttatta tatcacattt
    37921 tctttaaaag atacgaattt atatcatttc taatatacta aaatctttac tttttaccaa
    37981 aatttgttta aaaataggag aaaactattg cttttgacgc cccgtcatga tataatatat
    38041 ttatagagaa ggaggtgaga aagc
//
LOCUS       DAELJO010000014        35969 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELJO010000014 DAELJO010000000
VERSION     DAELJO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.014"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(15..521)
                     /locus_tag="KPB88_000667"
     CDS             complement(15..521)
                     /locus_tag="KPB88_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG0052972.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(597..998)
                     /locus_tag="KPB88_000668"
     CDS             complement(597..998)
                     /locus_tag="KPB88_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052973.1"
                     /translation="MKLGKIKGNENQIESEKSNATNQLLNLVLSKNLKDLRIKKGLTQ
                     EQVAKDLMTTKVSISRYENGTREPKIEMLDSLSNYYNVSVDYLLGKTLIENYTTTDEI
                     SKIIKSYESLPKEAQEHINSYIEFLVDRYKK"
     gene            complement(1264..1809)
                     /locus_tag="KPB88_000669"
     CDS             complement(1264..1809)
                     /locus_tag="KPB88_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0052974.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            1990..2205
                     /locus_tag="KPB88_000670"
     CDS             1990..2205
                     /locus_tag="KPB88_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052975.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            2236..3051
                     /locus_tag="KPB88_000671"
     CDS             2236..3051
                     /locus_tag="KPB88_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HBG0052976.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQTKLPTTYKEALQHLIEQVEVNEQLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQYQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSMLG"
     gene            3094..3261
                     /locus_tag="KPB88_000672"
     CDS             3094..3261
                     /locus_tag="KPB88_000672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052977.1"
                     /translation="MENKKIEEIKNALIGVNQTEWSLVKSFIDMYFSKKAAKLEIDDL
                     DNFDLYIKRKF"
     gene            complement(3258..3515)
                     /locus_tag="KPB88_000673"
     CDS             complement(3258..3515)
                     /locus_tag="KPB88_000673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052978.1"
                     /translation="MKVGDIMTKIYACLAGNWVNLCDDPECKMGSNHSSPYIWWEEGA
                     NVWSPSNKEEEHTMYQQDYVHIYYKGVDYRIHPIFIQIASN"
     gene            3574..3771
                     /locus_tag="KPB88_000674"
     CDS             3574..3771
                     /locus_tag="KPB88_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0052979.1"
                     /translation="MLIGDNISQILRKRDIKPYKLAKELNIDISGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            3802..4194
                     /locus_tag="KPB88_000675"
     CDS             3802..4194
                     /locus_tag="KPB88_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0052980.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            4181..4324
                     /locus_tag="KPB88_000676"
     CDS             4181..4324
                     /locus_tag="KPB88_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052981.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            4491..4733
                     /locus_tag="KPB88_000677"
     CDS             4491..4733
                     /locus_tag="KPB88_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052982.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            4812..5093
                     /locus_tag="KPB88_000678"
     CDS             4812..5093
                     /locus_tag="KPB88_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052983.1"
                     /translation="MKTKNEIIKDLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLQQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            5080..5718
                     /locus_tag="KPB88_000679"
     CDS             5080..5718
                     /locus_tag="KPB88_000679"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG0052984.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            5737..6627
                     /locus_tag="KPB88_000680"
     CDS             5737..6627
                     /locus_tag="KPB88_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0052985.1"
                     /translation="MDKVIIEKGNILSDGYGLMPRLVARDRWLTVGARMLYSYLSSFA
                     GINGTCFPTRDLICYELNISKDTFTKYKNELELSGYIKIHKNKSKQGKMQNNIYEIVF
                     DRTYIDECISRRGVKVNKKKKKPCPKNIDMEPCPTFPDTTQPDTENVDTNINSININS
                     FNSMYIDKPVDNYLKEFKKLYEENIGVVYPVTAEWLLEVSNEVDIRVFKRAIEICAER
                     MNMNLSYLKGILKKWKDANITTYEQLEAYKLQHENKKSKKTNSVVSKNKFANFEQTFT
                     QYSNKELDEIIKKSQKAKFK"
     gene            6647..7066
                     /gene="ssb"
                     /locus_tag="KPB88_000681"
     CDS             6647..7066
                     /gene="ssb"
                     /locus_tag="KPB88_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0052986.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            7096..7458
                     /locus_tag="KPB88_000682"
     CDS             7096..7458
                     /locus_tag="KPB88_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052987.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKSKTRSGWKVEYIKEE"
     gene            7871..8068
                     /locus_tag="KPB88_000683"
     CDS             7871..8068
                     /locus_tag="KPB88_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052988.1"
                     /translation="MNKFQKAVSQMVKQEEKENLWQGYENCRVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            8095..8505
                     /locus_tag="KPB88_000684"
     CDS             8095..8505
                     /locus_tag="KPB88_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBG0052989.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTVDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKKIRKR"
     gene            8529..9029
                     /locus_tag="KPB88_000685"
     CDS             8529..9029
                     /locus_tag="KPB88_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052990.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            9438..9614
                     /locus_tag="KPB88_000686"
     CDS             9438..9614
                     /locus_tag="KPB88_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0052991.1"
                     /translation="MEKRILKVLLSKSGSGSLSPKISLPATWIKEMNITQEEREVEVY
                     FENNEIRIKKKDLD"
     gene            10204..11151
                     /locus_tag="KPB88_000687"
     CDS             10204..11151
                     /locus_tag="KPB88_000687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052992.1"
                     /translation="MGSKTIKVFYYYPIIKTVDTFRNERILLYDLNSVLEKLKNLNVE
                     DRVFKLTDEENIQLKLIEKENENKWKLGFLKNSKDSIFKSKLEEVVKEAESLDDDEFI
                     GQECCMIYDKETGVISLQNNRKSVSFKNVSSFINEFIEKSHNRENVELYPITLKPEYS
                     NISDLDTINYRSVSLSFLDMSEIKMLAQEESNKSVEAIAKISNNLEALSGKIELSVGW
                     SKNRFLEKINLREIVSFFKRHPKLTRGIKVKSYDYNRDEIRVIDLIENKLYDEFPITV
                     TKNKVKTFEKIFSPMSECFNDTVEKTLDDCNKVMEEVYV"
     gene            11191..11703
                     /locus_tag="KPB88_000688"
     CDS             11191..11703
                     /locus_tag="KPB88_000688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052993.1"
                     /translation="MKYKKIILFNIFIIIFIILSKYLSFEKNIILYISFNTVGYNFKD
                     LLSISATILAVFIGFIATIATVLVSMCDKRIMVIIKRFGKTNIVLRSIKRALLSGIIS
                     LALIGIIYVNLDFNIILLRCIIIWVTLNNLFIFIRESKFLVFLVKHIMEQTFLDNEEI
                     VFKSKTSKKN"
     gene            11777..12217
                     /locus_tag="KPB88_000689"
     CDS             11777..12217
                     /locus_tag="KPB88_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBG0052994.1"
                     /translation="MADLTEKQKRFCDYYIETGNATEAYKKAYKNNNQRASESNGSRL
                     LSNDKVKNYIDERLKQIESKRIADAKEVMEYLTKILRNQEQEEVVIVSENGPEIIKKD
                     VSIKDRNKAAELLGKRYALWTEKVDLDGNVGVTIIDDVGNLNDG"
     gene            12207..13472
                     /locus_tag="KPB88_000690"
     CDS             12207..13472
                     /locus_tag="KPB88_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0052995.1"
                     /translation="MMDRKISEIINKNFYEFWKVSNSNKYLYHVLKGGRASAKSTHIA
                     FWLTMAMVKYPVNTVCFRKVGNTIMDSVYEQLKETIEIFGLTHLFQFKKSPMEIIFIP
                     RGNKFIFRGLDDPQKIKSIKSAKYPIAFAWFEEVAEIKTEDELSMVINSVLRGELPDK
                     LNYKIFLSYNPPKRKQSWVNKKFETHVLPKNTYVHHSIYLDNPHISKAFIEEANEIKI
                     RNEFKYRWEYLGEPIGSGVVPFSNLEFKTITNEEIFQFDNIRQGNDFGYATDPMAFVR
                     LHYDKKKRVIYFIDEIFGVKMSIRELASKIKSKGYDDFSVICDSAEPRSIAELREYGI
                     KALKAKKGPGSIEFGENWLDDLQAIVIDPNRTPNIAREFENIDYQTDKDGNVRAKLED
                     KDNHSIDATRYALELDMKTHGRERKYNSR"
     gene            13483..14937
                     /locus_tag="KPB88_000691"
     CDS             13483..14937
                     /locus_tag="KPB88_000691"
                     /inference="COORDINATES: protein motif:HMM:NF016988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0052996.1"
                     /translation="MLDLIDIIQMELTGLYGQEVIREMGEIIRLYDKYEGTGQHWIEE
                     EQDYKQTRKKTNYIKKLIKEEARFLFGKTPIFTIKPENDLDKDKAEEINQCINKILKK
                     NLFSDKLIKAARDCFIGKRIAIKLHADKESKSLKIMFVPSLEFIYEPFDNQFDELKKI
                     VFFHQTNQEVEKDKQRIWKQKYEMVNGKCILSEGIYNGYGLCIEEIVSDTDLKLSGIP
                     CYVILNDGLLGDLKGESDIEEIFDNQMAYNKLASEDIDTLKKGMNRIIYGVDVEEESS
                     KHFKIKPGAYWDVETSQTADQKQAQINTIPTDFGYDTRIENSLNRIKSDMHEVLNIPL
                     INNQDLKGMMTSGKSMKALYWQLITRCEEKMKSWGPALEWLIQAMIEMIEVYNIVKIP
                     ILDKDSYEVIVENQYPLQEDEDSEKLLDIQQVNAQAMSRKTFIKKWNNSNDDIADEEL
                     QQISIERQILEESFNLEEETEVIDTEESEEVVDE"
     gene            14937..16355
                     /locus_tag="KPB88_000692"
     CDS             14937..16355
                     /locus_tag="KPB88_000692"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052997.1"
                     /translation="MANKFNKAMKNAERARDISSNRTTKKIRKLYKDIANEYAKKLNR
                     VNSNTLTEQYLRESIIYLNKEYDRLGKKLKKDIESEISKVIKTTTDEQLSFFNNICDN
                     YSVNLKPQFTDMFSKVHEDVLRQVISGSMYKDKLKLSDRIWSNIDKTKKDLDYIVSRG
                     LAEKRGSYDIAKDLEKYVNPKVKKDYDWSKVYPKSNKKIDFNAYRLASTYITHAYQKT
                     AKESCRKNPFVKGIKWMSSHHPRMCKVCADRNGKTYIPEELPLEHPLGKCTFEYDIPM
                     SMEDIGKELRSWIDGEENSKLDEWFDEYGLDFAGIEGKVNKNKEPTEDEMLALYKYMG
                     GDAYKINEKLRRNIKLTEEDEWFINNLDKVLDKMPNYEGDVTRSLYFYNKESLIEFLE
                     SHELRKVKEYKEFLSTTKGETYNPNAQVEIYILDSKKGKNISMYNDKEKEILYERNSK
                     FEVMEIEEINGKYFILLEERDE"
     gene            16348..16515
                     /locus_tag="KPB88_000693"
     CDS             16348..16515
                     /locus_tag="KPB88_000693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0052998.1"
                     /translation="MSKEDKYKEPFSHPRWKDIPWGETIREVERTEEEQAEIRKKFRK
                     KLKELGVLKED"
     gene            16650..17285
                     /locus_tag="KPB88_000694"
     CDS             16650..17285
                     /locus_tag="KPB88_000694"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0052999.1"
                     /translation="MEELLKKLGYQDTDIKNIIESMKKEKIYTSKEENIDERYNKLKE
                     QKIALEEQIKGANDTISDLKKNSKNSEDIEAKVKEWENKYNELDSTSRAKIRNMTIDY
                     AINSKLSGVNEKYRKLLCKEFDTSKMEVKDTGEIIGLDEQFKDISETYKEWFESSTPS
                     NTGSPGNFPRKSNVVNNPFIKETFNLTEQGRLLKENPDKAKEFAAQAGINL"
     gene            17303..18271
                     /locus_tag="KPB88_000695"
     CDS             17303..18271
                     /locus_tag="KPB88_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBG0053000.1"
                     /translation="MAVTKLSDVIVPELFNPYVINKSMELSALYQSGIITNDASLNAL
                     ASQAAPVVNMPFFEDLSGESEQIIEDADLTPNKITSSQDAAAILRRAKMWAATDLSAA
                     MAGKDPMAAIASLVGSFWARDMQKELIAVLNGVFLAANMTTNKLDISAGTGNAAKWSP
                     SAFIDAQQLLGDAQGQLKAIAMHSATKSALKKQNLIETIRPDVGPDFEVYQDKLVIVD
                     DGCPVSKEGVYTSYLFGSGAIALGNGNPVGFVATEIDRDKKKGSGVDYLINRKTYILH
                     PRGIKFTNASVAKTEGPSRLELAKGENWTRVYEPKQIRIVEFKHKL"
     gene            18297..18551
                     /locus_tag="KPB88_000696"
     CDS             18297..18551
                     /locus_tag="KPB88_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053001.1"
                     /translation="MDLEILKNIKTELREEQSPFFSDDEITYYYNKNNQDFNKTMYEL
                     CILKAENDSITLPGGLSMPENKLYWLTLAKKFKTNGSRCL"
     gene            18548..18910
                     /locus_tag="KPB88_000697"
     CDS             18548..18910
                     /locus_tag="KPB88_000697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053002.1"
                     /translation="MIAQKVKPKIIKAINKMPTKATVKRVGVNEFGEPSDEENIVCNV
                     IGLYNEGSSSISQITKDKGIVIKDKEQYLMVVCDEDTVKIKQGDFMYLDNNKFIIQDL
                     GNQNRMNIYFDLKLGKVR"
     gene            18912..19346
                     /locus_tag="KPB88_000698"
     CDS             18912..19346
                     /locus_tag="KPB88_000698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053003.1"
                     /translation="MSKGFKFDTSKLLNALVSREMKTKAALGAYADTSSQLLESTAKN
                     DRPWKDHTHDAKNRIHGSWEWQGDTIRIALSHGVDYGLYLEKGTGPHVIEARPGSYLF
                     WDGASHPVKKVNHPGSRPYPIIMPTIEKCGPSIIRGLDVILK"
     gene            19354..19698
                     /locus_tag="KPB88_000699"
     CDS             19354..19698
                     /locus_tag="KPB88_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053004.1"
                     /translation="MFKKIYKHLKTKGFNVYSIGQHQGLCIEPFLVIFEKGPLQTTEK
                     NIIKDLFEIYVFYPIGQYSKVSEYKISVESVMDEIVGIKQAYEALPILIDDEKQAYFT
                     RLSYYENKQIRR"
     gene            19704..20510
                     /locus_tag="KPB88_000700"
     CDS             19704..20510
                     /locus_tag="KPB88_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053005.1"
                     /translation="MAVQILNQYPLTDVVLVQIETVEQNPVTYTFDTSDEIGTEEIIS
                     EGEELTLKIKKKIIANRAAEDTSLGYDLTLKDNVFCPEILQIMQGGTIEKEEDGSFKR
                     YLAPEVGKTFSKKSFKTIIYSSVVGPGGDTGQFAKTTFPNCKGKSVPLNFKDGEYYSN
                     EYVINSRPNTGQSPYEVEIVEELPNGYEVTKVFLDSASVSGATAGDKKITGLTAGKIY
                     KVTVNSNIKYTLADGTLTDTESDKAALTGTEIIGLTNGETYKVEEVSVSI"
     gene            20569..21045
                     /locus_tag="KPB88_000701"
     CDS             20569..21045
                     /locus_tag="KPB88_000701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053006.1"
                     /translation="MNELKVTSLEELKKIKLTEVIEVGRFLDGTMLVAEVKQPDLMAL
                     AMAGKIPNSLMTASMSLVEEKESKDSTGEKVLKKMNDESNFSKEMFEMMDIVAKEVLV
                     NPTYAQIKKIGLELSIEQRLTLFNRIQGGTKSLENFHQESTDIEDSKSSDNVQQDA"
     gene            21038..21235
                     /locus_tag="KPB88_000702"
     CDS             21038..21235
                     /locus_tag="KPB88_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053007.1"
                     /translation="MPSEILRIKDEYTSFCFDEACMFLVDAIKNDKKLKFKENSNTSK
                     ENIKRTTFVDMALKKKEKDRK"
     gene            21323..21865
                     /locus_tag="KPB88_000703"
     CDS             21323..21865
                     /locus_tag="KPB88_000703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053008.1"
                     /translation="MKINKKILSLVLSLVIGIYAVGCSSPSSSDDSSKKDAKTEQKKD
                     EKKEYKFGDTIEMKDYDEGTYKLTFEGVRSTDERNEFAEIEAKKVVFVDFNYENVDCK
                     EAVFVTEGIEYQVADGDGNVLQTYPVDDSTRMQQETTAGTKSKGTTAFAITSDSNAVK
                     IIVKDKVNNKVMGEINATLE"
     repeat_region   22123..22346
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=22123..22150
                     /rpt_unit_seq="gtattatattaactatgtggtatgtaaa"
     gene            22387..22758
                     /locus_tag="KPB88_000704"
     CDS             22387..22758
                     /locus_tag="KPB88_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053009.1"
                     /translation="MSLFGSKITKEEKEKNREETAKYYERLKNMTKEEKKEYEIQEFM
                     KKYQLENLDEKDLIVLKRIASDLFGNGLIKAGMALSFTDAKEQAKIGYLSALTEQNWM
                     IIRQLAQLNTNIEKLIEDKKE"
     gene            22838..28558
                     /locus_tag="KPB88_000705"
     CDS             22838..28558
                     /locus_tag="KPB88_000705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0053010.1"
                     /translation="MSLELGTAVGYLDLDGGKFFKALADAAKQLSQFNSSTKTTGSAM
                     TAMGKQISDTANSLTQIGRQSALAASELNKIASTSSGPFSSLKNSIKQTENELKTARS
                     TIDAYAQGISKLTSEIDKSKQKYTEIGNKIERYEQQLERCNSMYGESSEQSQRYREAI
                     ERLKNSQTQLGNEIENGEDSLTDMRTAMNNAEAEANRLSDSLRSMPFDAIGGKMRDIG
                     QTLTSTVTTGLIAMGTAAVTAATNTEQAMSVVNSILQLNTEKVKGGKSEWDAYSSTLK
                     AGANEIGMAYDEYANAAYNAISASVKQADVTEFLAQADKLATAGLTDLAKATDVLTTV
                     QNAYGMSQKDMAHVSDVLIQTQNKGKITVDELASSMGKIIPTAKSLNVSVEQLGAGYA
                     ILTAKGIAGAEATTYMNAMFNELGKSGSKADKILKKLTDKGFADLQKEGKSTADVLLI
                     LNDYAKKNSLSLSDLFGSAEGAKAANVLLGDAVNETTGEIIEGTKSADSFNQMLGDMK
                     NSTGLCDKAFEQLDNTTKTKLEDALNSAKNMLSDLGEVMMPVVAKVAEMATAFMKSVS
                     EMAKSNPSFTRIVVSIGAVVSAIGPLLVVLGTMAITIPKAIAAIGTAGKAFTFLSTSF
                     AIAREIITPASVGASAGMIKMSKAMTALMGPMGLVVAGIVALGVAFVALNKHMSQDSI
                     QVSERFGKNISDGTQKAVGAFMDLEEQTTTTLNQMLWSGETVSTEMKDTLISNFDSMS
                     SQITSKLNETKESSKATLQEMFVSSTTLSDKEKEALIKNTDEAYSNKEKKIEESAARV
                     KEILTKASEENRSLTQSEADEINRIKTEMLNTAVNVMSESEAEQAAIMERMRVNATNL
                     SAKQAADVVKNSIKQRDETVKSANEEYNERLKLAAQLRAEGGKENEELANKVIQEATK
                     QKDEAVKKAQEMHKEIVSEAQKQAEGQVEKIDWATGEIKSRWEVLRDEIKKNIDTSQS
                     DFDTWCSNTDKGFADTWNKITQGVSNAWNGITESLSNAWNSIKEGVSSAWNSVLEWTT
                     NTWSNITSFLGELWESIKEMAINAWNGIVSSVTDAWNSITEFLSPVTSFISEMVKMAW
                     ESIKETAITIWQSISDIIQSVWDFIKSIVGLGVEIVKALIRAAWQGIKDITSSIWEGI
                     KNVIQSVWDAIKPYVMGAVDAVKTVVTNAWNSIKDISSTIWNGIKTVVTNAWNGIKSI
                     ASSVSNAVKSVVSSAWNGIKSITSSIWNGIKTAVSNAWNNIKSSATSSVSNVKNTTTA
                     GFNSLKSAVTTIWNNIKTAISNVWSNIKTAVSNGANAVKSVCSSVFSTISNILTAPFK
                     TAQKAISGILGGITSKINGIKDKVSGLFRSGPIIPQNSNDFIQKDMLPTPMMFRVSVA
                     GFASSRGSILDSINKFSNAMTKGMGSSSSGIASQFKKAGEKSAKAYLDGLSNIEQGLT
                     NTLRTVQAIIGNGNIKEAQEIKKFNKEVQKLQEEKAEEIAELDKEHKKKQKERNEKEN
                     EEIKEAEAKKYKNKKEKLAALKKIKDKYNKESVKDQEEYREKLEKINKKYNKKEKEDT
                     EKHQEELQKLREEKIKAERDFNKKYNNIKEDYAEKVANLDKKYIEDQKKLNEEYQKIY
                     DSRVKSLMDYTDLFSSVTFEEIDNDELMENLEEQVDVLRKWDTDMADLSTKVGKDLYE
                     ELLAKGPQAHNEIEAINKMSKEQLEEYERLYQEKKKIAERRAKEDTEDEKYRIEKEIE
                     ELKKTYDAEYRKLGQEMQRSIEEQIESFKDKFGVVPAMFMETGKDSMQGMIDGIKSMQ
                     GALGKVLSDIVNSINKQLSNIGFKDVNIPTGGRKEAARFRNEIENLQSIAYSDTLARG
                     IASNNLLKDATVNINNNSKVDSEKSSDKKVELTLHIEKFINNTKQDIEQLGSEIAFIT
                     NRKLKF"
     gene            28686..28835
                     /locus_tag="KPB88_000706"
     CDS             28686..28835
                     /locus_tag="KPB88_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053011.1"
                     /translation="MNNFLLNVIAGVIASLIFCLISKIFAKVKSHSVRKSGWEFDLKI
                     KFHKK"
     gene            28996..29751
                     /locus_tag="KPB88_000707"
     CDS             28996..29751
                     /locus_tag="KPB88_000707"
                     /inference="COORDINATES: protein motif:HMM:NF017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBG0053012.1"
                     /translation="MALQYCVSENELVSFQYGEINSRDFGIVITDINELASPERRFER
                     IEIPGRNGSLILDEGCYSNFNLEIECYIDVDGKDINILASEIKMWLQTDFSYKNLIIS
                     TDPNYYREAVCINKLDLEEIIKDLGYFKITFECKPLKKELVNNLITINESDSKLINTG
                     MASYPLIKVFGNGDITLEINNEVIELTEVQGHIYIDCELMNAYKIDIHTNGVVNENSR
                     MFGDFPTFKHGENIITYSGDVEKIEIEPRWVVL"
     gene            29753..32521
                     /locus_tag="KPB88_000708"
     CDS             29753..32521
                     /locus_tag="KPB88_000708"
                     /inference="COORDINATES: protein motif:HMM:TIGR01665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0053013.1"
                     /translation="MLRLYKANETNFKNNGLGILKDAFETKVTEELNGLFELEFTYYV
                     GSFLFDEIDYNKIVMADASPRLKNQLFRIYYISKELDGKILVKAEHISYDLLNNFIEN
                     LELKNVTCEEALNQIFRSCTEENRFVGYSDITGNKDFSISCVSPHNAISDIQELFNNK
                     SKLKRDNFNISLLNNIGESNNVLLAYRKNIIGLTATYDTQEVITKIYPYATKKSGKNK
                     KITLPEKYIESKYINNYPTQRIVAVDFSDDDVKNEESLRNKCKDYFIENNVDLPKVTY
                     KVEFVDLSTTEDYKSYKMLETVNMDDEIIVRDYNLGINATARVVKTEYNPVLKKYNSV
                     EIGDLVNHFKDERIDDLEEKIDKVQNNVDNIVIESDNFPDTLPEPSNVTALGLWSMIQ
                     LDWTFDNKLYYNYEVYASQIKGFEPDTIGYTNRIYVGQASSLLHEVKPMQTWYYRVRA
                     GNTHDNYNEFSNEVSATTRKLSDAAEYFEEAAIGHAVIRDLDADKINVGKVKGQYIEA
                     KNLVVVDGNSQTTLNIDSFGNVHIGATTFTLKGKSLESIIGGEIDDITQLEIFNKLTN
                     NGLAKGLYMVGNELYLNASYIKTGTLEGQFINGRNLTVRDNDGYTTLQVDSNGKVNIR
                     ANELSIGDKNNYESVLTSDQKAVFDALTGNRNCGIYLSGSRLYINADYIDTGTILCDR
                     IGASSSNPFILLFEGNGAKCALDATAQFGVGIGKAMRMKYNDYSYIYVSDDVISGYLD
                     GEEVFEFGYQDEKSYINTGLNTQHINPQIDGWYNCGSNRKAWDYLVCNNLNQLARTAA
                     TSTRMMKSTFNEEISNNCIDFVKSSLVQSDVFTPYNLKSMKNNNIEHRLQVDIDSSLD
                     NPISKYIFKDVSDEAGEGVYAQDITSHLAVLQLSLQKTISNFENYKDITSKKIEELTK
                     RIEVLESR"
     gene            32528..34747
                     /locus_tag="KPB88_000709"
     CDS             32528..34747
                     /locus_tag="KPB88_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053014.1"
                     /translation="MRDRIYIVDINTKMYQIARYKQNDNNVSYNMRIVQDSIDVDLTG
                     YTALAFFNLPSGRVTQKNCTIEDSTVYTELSHIELSEEGDVISEITLYKDDKVVTTFS
                     TIIKVEKSINRNAIEDEPSWDIIKDILNVLSYEEERQENENVRKSNEEIRISSENVRI
                     GNENVRIESENQRKDSEVERCESEEVRKTQEVTREANEETRKTSETTREANEEIRKTS
                     EITREANEEIRKTNEVDRESSEETRNTNEETREENETVRESNEEIRKTSEETRESNEN
                     TRKASEEIRNGSEETRVANEVARQESETTREENEDLRKSNEETREENEEIRKSNENTR
                     KASEEIRNTSEETRNTNEEARKASEEIRKTSETTRNTNEEARKVSETTRETNEETRKT
                     NEIIRQNSETKRKENEELRESNEGTRELNESTRQTSIINMQKKVDDKVDEANNKIVEV
                     NTAKTDMTTTVSNKITEFEKRFNELENLDTAGEILQSKETTDGQIKDTLKERLTYDFN
                     KLDLKIEQLIAGGINVAFNKSYDTSEWIEVDGGFELSVEHNLVTQKILVSSIDKLNRK
                     SLITSYKIIDDNNIVLFNEVAIDIEVTVVNGGSAIELIKFTINDDIESLESAYSSIET
                     KRLLKDGLDKKANLEHTHDIYVEKVEGKELSTEDYTTEEKEKLSQLDTYKNKETKFNT
                     NGSIIEILDNNIKYITKFNTNGSISKEKYINDILINTTITNFKNGMIEETVTNEGVI"
     gene            34747..35652
                     /locus_tag="KPB88_000710"
                     /pseudo
     CDS             34747..35652
                     /locus_tag="KPB88_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT        15159 a         3752 c         6602 g        10456 t
ORIGIN      
        1 tagaataaat gagtttaaaa aatatcctca tatatgtaga aatttggcaa attagttaat
       61 actaaaaatc tattatttcc taagtccaac atgcttttct tttttgatag aaattctaat
      121 ctttttagta agaagttgat gctaacttgt aattcttcag ctatttcgta tacacttgta
      181 gcgtgtgaat taataacatg tattatttct tcttctgtta taagaaattc acatgcccat
      241 tttagtgctt tgttttcagt tttgtctatc aagattttat ttttgtaact gttttttgaa
      301 gatacatagt ttccaacact ggtaaaatga tgtcctaatt cttctgctaa gatttctatt
      361 agtttagcat tgttttgttt taatgaatta agtaatgata taatttttag tccttgtctg
      421 tttatataca atccttttat gtcatctgct attttgtcag tgtagtaaat ttctatctct
      481 tcattatttg ctaagtctaa aagtgcgtct agtttgttca ttgaaatccc cctataaaaa
      541 gaatgtatgt gttgcttttt tatatataaa gagcaggagt gaactgctct aaatattcat
      601 tttttatatc tatcaactaa aaactctatg tagctattaa tatgttcttg agcttcttta
      661 ggtaaacttt cataagattt aattattttg gatatttcat cagtagttgt gtaattttca
      721 attaaggttt taccaagtag ataatcaact gatacattat agtaatttga cagagaatct
      781 aacatttcaa ttttaggttc tctagttcca ttttcatatc tactaatgga tacttttgtt
      841 gtcattaaat ctttagcaac ttgttcttga gtaagacctt tttttattct taaatctttc
      901 agatttttcg aaagtactaa atttaacagc tgatttgtag cattagattt ttcagattct
      961 atttgatttt catttccttt tatttttcct aatttaatta atagttcttc tagtgttaag
     1021 tttaatccaa aacttatttt ttctagggaa tccagtgtag gttcaattgg attcccattt
     1081 ctagggtctt tacctttttc tagcttatct atataagcat ggctaaggtc acataaatct
     1141 gaaaaatccc taagagataa tttatttaat cttctatatt cttttataat ttcttttagt
     1201 gtttgcattt aacttacctc ttttgaaata tctagtatta taaatttatt aaagtgtaaa
     1261 tacttacttc ttatatttat tcattaaaaa ttcaatataa tcattaagct gttcttgagc
     1321 ttcttcaggt aaatcctcat gtggatttgc tttgtgtgcg gctacagtgt ctatatgatt
     1381 tctaacaagt gttgtaccta aaagataatc tgttgtaaca tcaaaataat gagcaagctt
     1441 tataatatca tcacttttag gtgaaattat attattttca tatttagata ataaatcagt
     1501 acttatttct aattttttgc ctaatatatt taatgtgaca cctttttcct ttattaattc
     1561 tcttaatctt tttgagaaaa taggatttaa agattttact acttgattgg gattttttat
     1621 gtctgtttta ccaagtaagt aatccgttga tacatcaaaa taatttgaac agtcttcaat
     1681 aaaagattgt tttggctctc ttaacccatt ttcaatccta gatagagtgg atttatttac
     1741 atttaaatcc atacttaatc tatctaaaga tattcctttt tcttctctca gttcttttaa
     1801 tctaaacata taaaatcatc ctttattgta gttttcatta tagcaacttt tatttacatt
     1861 atagcaactt tttttgttat tacaattaaa attgccaaaa aagcaacaaa aatattgact
     1921 tttaacattt ttgttgctat tatataaaca agaacttgct tatatagcaa aaagttagag
     1981 ggtgaaaaaa tgtatgtcaa tagattgaaa gggttaatga aagaaaacca acatacgcaa
     2041 aaatttgttg cagatttgtt aggtcttagt ttatttggtt ttaggcttaa acttaatggt
     2101 aaaaatgagt tcaaagcaaa tgaaataaaa aagttgtctg aattatatgg agtatcaaca
     2161 gactattttt tttcagaatt agttgctaaa atggcaataa aatgatggac attttataga
     2221 gaggagtttg taaatatgaa agatttaaaa atagtaaaag ttaataataa actaacaact
     2281 gatagtaggg atatagctct aatggttgaa aaagaacaca agattttact aagagatata
     2341 agaaactata taaatcagat ggaagaagcc aataaaaaca tgagtacaga tttgtaccca
     2401 tctgattatt ttattgaaaa tacttattta gatgattata aaagagagaa gccatgctat
     2461 gcaataacaa agataggttg tgacttcata gcaaataaaa tgacaggcat aaaaggtacg
     2521 gcatttacag gaatatatac aaaaaaattt gaagagatgg agcaagtatt aaaaaatgaa
     2581 caaactaaac taccaactac atataaagaa gcgttacaac atcttataga gcaagtagaa
     2641 gtaaatgagc aattacaatt agaaggtaaa atgaaagacc aagtaataaa agaattaaaa
     2701 ccaaaggcag actatacaga tatgatactt aaaaacaaag gtctagtcac tataactcaa
     2761 atagcaaaag actatggaat gagtggaaaa gaaatgaata aaatacttca tgaaagaggg
     2821 attcaatata aacaaagtgg acaatggctt ttatataaac agtatcaagg caagggatac
     2881 actcattcag aaacaataga tataactaga agtgatggaa tgactgatgt aaaaatgaca
     2941 actaagtgga ctcaaaaggg aagattgttt ttatatgact tattgaaaac aaataacata
     3001 ttaccagata tagaaaaaga atatagttat caaacttcaa tgttaggtta gtgatttagt
     3061 aaaacacaga atattcaaaa aggagtgatt aaattggaaa ataagaaaat agaagaaata
     3121 aaaaatgcat taataggggt gaatcagaca gagtggtctt tagtaaaatc ttttattgac
     3181 atgtatttta gtaaaaaagc cgccaaactt gaaattgacg acctagataa ttttgatttg
     3241 tatataaaac gaaaatttta attactagca atttgtataa agattggatg gattctatag
     3301 tcaacaccct tatagtaaat atgaacataa tcttgctgat acatagtatg ttcttcttct
     3361 ttattagaag gactccatac attagcacct tcttcccacc atatgtaagg tgaagaatgg
     3421 ttactaccca ttttacattc tgggtcatca catagattaa cccaattgcc agctaaacaa
     3481 gcatatattt tagtcataat atcaccaact ttcatataaa tttggaatat attccatatt
     3541 tatattatac catgtagaac tgaggtgaat ccaatgttga taggcgacaa tataagccaa
     3601 atcctaagaa aaagagatat aaaaccttat aaattggcaa aagaattgaa tatagatata
     3661 agtggtctat ataaattgtt gaagaataaa aattctaatc caactataga taccctaata
     3721 aaattagctg attatttaga tattacatta gacgaattag ttggaagata aataattaaa
     3781 aagaggggag agaattttca catggatatt tctgaaagta tagcaaaaca atttagtgat
     3841 agtttaaaaa gtttaataga aatagagata aataaacaag aaacagatag agttaagagt
     3901 caaactgttg aacaaaaggt aaaagtactg gagcctaaag atatagttgt tttaataaaa
     3961 agaggttatc caaattattt gataacagta gaagaagcaa ggggaatttt aaaattagat
     4021 acagttttta tgcgtaggtt agtaagcaca ggtttgataa aatcactggc tagaggtgat
     4081 ggtagaaaaa tttcaagata tgaagttgat gattttattg aaagaaatca aggtaaaaat
     4141 ttggatgaac ttttaaaagt agcagagagg ggggatgaaa ttgttaagcc ttaatactaa
     4201 taagaataat atagtaactc ttaaaaaaga tggaaaagct atagcagaca tagtatttaa
     4261 agatattaaa actggtaaga aaatatcggt tggagtatta aataaaaaag tgctggtcaa
     4321 ataaccaaca cacaaaaaaa taaaaaataa aatataacac aaacaaatta tagcacaaaa
     4381 agaatggatt tagaataggt aatataacat tctaaaaggt ttaatgtgta ataattgtag
     4441 caatttagaa tgtagcttat atagatagta ataaattggg agggatttaa atggaagcag
     4501 ctagactaat agcaataggt caaattaaac aagcagaaaa agaaatatgt aaattacaag
     4561 gtacaaaaaa taatagtagt ttaatgtggt gggaagccgt aaaatttgct agtcaaaata
     4621 tattagaggg tcttgaacat gacatcgagt tagaagcttc tattgagttt agagaagcta
     4681 tgatgtatca agaagaactt gaaaaagata gaccaataga tgtccagata taaaaaaaga
     4741 gccttcgcac aggctctaag tgaaaataag ttacaaaaat tataggtata ttataacata
     4801 agggggaata aatgaaaaca aaaaatgaaa taattaagga tttagaagat agattatttt
     4861 tattaagatt tacaacagta gatgaagtag attgggatgt aaaatttgga caaatatcag
     4921 cattagaatt ttgtatagat aaacatagaa aaggatgcac tttgcaacaa ttcaaagaac
     4981 atttagacga atacaaatta caagggaact atggtgatta tatagatggt tttgtgtcag
     5041 ttttagaaag aaatattaga gaaatggagg gagaaattga tggaagtgaa taatatttac
     5101 ataaaattga tggatgtaag aattaagttt aacaaattag atataaaaaa gagtggtcaa
     5161 aacaagtttg ctaactttaa atattttgag ttagcagact tcttacctca agcaacagag
     5221 ctgttacaag aagctaaatt atgccctata gtgaccttta caaatgatta tgcaactcta
     5281 acattgatta atggagagaa accaacagaa gagataatat ttacttctcc aatgagagaa
     5341 ttacaattaa aaggctctaa tgagttacaa gcattaggag gaattgaaac atatcagaca
     5401 aggtatttgt acattcagtt attaaacatt acagaaaatg atacttttga tgctactagt
     5461 ggaaaagaag attataaaag aaatgactta acaaactcct ctattaaagc aagtgaaaat
     5521 ggtcaaataa aaataagtca aaatcaaata aaaagattgt tttcaatagg aaacacaata
     5581 ggcaaagatt cagatagagt aaaaagtgaa gtgtattata agtttaataa agaagttaaa
     5641 gatttaagta aacaggaata tgaccagata tgtgttggat atgagaaatt acaaagagaa
     5701 aaagggataa ttaagtaggt gaatctcttg aataatatgg acaaagttat tatagaaaaa
     5761 ggaaatatat taagtgatgg gtatggtctt atgccaaggt tagtagcaag agatagatgg
     5821 ttaactgttg gtgctagaat gctatattct tatttatcca gttttgcagg gataaatgga
     5881 acttgctttc caactagaga tttaatttgt tatgagttga atatatcaaa agatacattt
     5941 acaaaataca aaaatgaatt ggaacttagt ggatatataa agattcataa aaataaatct
     6001 aaacaaggta aaatgcaaaa taatatatat gaaatagtat ttgatagaac ttatatagat
     6061 gaatgtatat ctagaagagg tgtaaaagta aataaaaaga agaaaaaacc atgtcctaaa
     6121 aatatagaca tggaaccatg tcctactttt ccggacacga ctcagccgga cacggaaaat
     6181 gtggacacta atattaatag tattaatatt aatagtttta atagtatgta tatagataag
     6241 cctgtggata actatttaaa agaatttaag aagctatatg aagaaaatat aggagtagta
     6301 tatccagtca cagctgaatg gttattagaa gtatctaatg aagtagatat aagagtattt
     6361 aaaagagcta tagagatatg tgctgaaaga atgaatatga atttatcata cttaaaagga
     6421 atccttaaaa agtggaagga tgcaaatata actacatatg aacaattaga agcatataaa
     6481 ttacaacatg aaaataaaaa gtcaaaaaaa accaatagtg tagtaagcaa aaataagttt
     6541 gctaattttg aacaaacatt tactcaatac agtaacaaag aattagatga aattataaag
     6601 aaaagccaaa aggctaaatt taaataatat tgatggaggt attaaaatga atcaagttgt
     6661 attagttgga agattaacta gagacccaga actcaaatac ataccaggaa caggtacagc
     6721 agtagcatca tttacaatag ctgtagacag aaattatata aataaagaag gaaaaaggga
     6781 tactgatttt ataccaatag aagtaatagg taaatcagct gaatactgtg caaattacat
     6841 aacaaaaggg aagctagtag cattagaagg gaatataaga gttgacaatt atcaaactca
     6901 atcaggtgaa aaaagaatat ttacaaaagt cagtacaaaa tcagtacaat cattagaaag
     6961 caagaataaa tcgagtaatt catataaaga gagtgtacaa gatggaacca taggactaga
     7021 ccctcaagga tttgaaatta tagatgatga tgagttacca ttttaatctg aaaaattaaa
     7081 tatgaggtga aataaatgtt taaagtagaa aggtatttta gtggctcagt agtggacaac
     7141 cttattgaag atgaccttac atgtagaaac tacttagcat tatattgttg tttgttggga
     7201 attacaaaaa atggaaaaaa gatatatcct aagccagaaa aaatgttagc tgagtttgga
     7261 gtaaagaagg acagaaaaat aaaaaaagag ttaccagtaa gaattagaaa tgttaataca
     7321 ggggaagtaa aagaatttga gtctatagat ggtgcagcct gttttttaag attaaaatat
     7381 caagcagttt atcaagctat taaaaagaaa agtaaaacta gaagtggctg gaaagttgaa
     7441 tatattaagg aggaataatg gaagtttcaa ggacagaata tacaattaaa agagcaaaag
     7501 agctatatga caatggagag gatatattta ttgctataga taaggctaga gaagaatatg
     7561 aggagatgat taaaagtgaa tatcttagct agtgtaatat tagtaatagg aagttttata
     7621 gctggtagag tttatgagta tagattgaat ctaaaagagt gtgaaaattg taataacaaa
     7681 tatcctgaaa aatgagaaag aagtggtttt atgaataaaa gaataatttg caattggtgt
     7741 ggtaaattat tttacatctc aaaacagtct aaaaaaattt attgttgtaa aagatgtgag
     7801 agaaaggcta atagaagtaa tagagaacag caaaattaat tttaaacaat aacactatgg
     7861 gggaatagca atgaataagt ttcaaaaagc agtttctcaa atggtaaagc aagaggaaaa
     7921 agagaattta tggcaaggat atgaaaattg tagagtaggt agaagtatat caagttcaat
     7981 aagaaggtat gtaaaaggat ttgaaaagtt tggatatagt gtacatgagg tttatgaatt
     8041 tataaacgat attaataagt atgagtaact ttaagtgata aaggaagtat ttgattgagt
     8101 aaatacaata ataagaaaat tgtaatagat ggaattaaat ttgatagtaa agatgagtca
     8161 gagtattatt tatatttaaa agaaaaaaag gaaaatggag aaataaaaga ctttggactt
     8221 caacaaaagt ttgaactaca acctaaattt aaaaaagatg ggaaaagcta cagagctatt
     8281 acatatacag ttgattttgc catatacaaa tggaatggtg aagtcgttta tatagatgtg
     8341 aaagggtata gtacacagca gggtgaactt agaaaaaagc tttttgacta taaatatcag
     8401 gacaaaaaat tgatatggat tgctaaaagc aaaaaatatg gggtagatgg ttggatagaa
     8461 tatagtgaac ttaagaaaaa gagaaaaaaa ataagaaaaa ggtagcttaa ataaatagga
     8521 gtgatgttat ggcaagtaaa gttaaaaagg agttttttat ggcaactaaa aaacaccttg
     8581 agaactacaa acaactacat attaatattg aaagtctaaa acttcaaata aaaaatctca
     8641 aagagttcca tttaggtgat tttatgcaag gtttaagcta tgacagcatt cccataagta
     8701 agactaattc aataagtaat caagttgaaa atgagttaat taatcttgaa gaaaagataa
     8761 tagaaaagca gatagaatta tatgaaatgg aagcactaaa atatacaata gatgtatcca
     8821 taagcaattt aaaacctata cataaacaaa ttataaggta taggtatatt gaaggcttag
     8881 aatggagttt aatagttgat aaagtatact tagaagaaag acaattaaga gaaagagcta
     8941 atcaagccat tagttcaata tcaatagcct tgtttggcaa gaaagcacta atagagcaag
     9001 aaccattatt taagatgtta gatttatagg cagtgaatag ctgtctattt ttttactaaa
     9061 aaaaggaaaa aaggtattga ttatatcggt acgacatgat ataatataaa tatagaaagg
     9121 aggtgaaaga agtggtcaaa aaaataaaag agttcggcaa agttgtcaaa gcccttaccg
     9181 aacttgctct tgaaataggt acactaatag ccgttataaa aatggtatta gatagcctat
     9241 aagactttag aaggggagtc agcaccttcc cttctaatta aataataata catgaccact
     9301 caaaatacaa tgggaaaata taaggaatta attacagagt tagggaaatt aagttttggt
     9361 atagttaaat taattggtgc aatagcatta ttagtatttg ctataaaata tttattttcc
     9421 tagagggaga gaaagaaatg gagaaaagaa tattgaaagt actcttatca aaaagtggct
     9481 caggttcact aagtcctaaa ataagtctac ctgcaacatg gattaaagaa atgaatataa
     9541 cacaagaaga gagagaagtt gaagtttatt ttgaaaataa tgaaattagg attaaaaaga
     9601 aagacctaga ttaaattcta ggtcttttta ttgccgtttt tctgccgatt ttacaattta
     9661 aagtgtgaga taatagtatt gtggaaatga atatttctct ctcaaaacta aatatatgtg
     9721 ggctaggtaa agggattcac ctagcttata tgaacagact aggcagggca tgaggatgct
     9781 gtaagttcaa ttcttactat gttcaaactt attaatacac tatatgtaga tatgctggat
     9841 taaaacggaa tttaattcaa atgtctaaaa gagtggggct tggtaacctc actcaatttg
     9901 caaggactgg tgtgtaatct taggttcgat tcctaaaact tgctccctta aatataatat
     9961 gtatccccaa aaaaagactt agattaaatt ctaagtcttt ttcaatttaa taaattatat
    10021 gtttattatt ttgtatccaa agcttttgat tttaacttat gaaataggta catatttatt
    10081 aaagcaatat ctttatttaa tcaaattatg aggaattatg taattgtcga aagattgtta
    10141 gaggaatgaa ttgaaatatg tagaatataa atttgtagaa taaagatttt ggaggaacta
    10201 accatgggga gcaaaacaat aaaggttttt tactattatc ctattattaa aactgtagat
    10261 actttcagaa atgaaaggat tcttttatat gatttaaatt cagttttaga aaaattaaag
    10321 aatctaaatg tagaggatag agtatttaag ttaacagatg aagagaacat tcaattaaag
    10381 cttatagaaa aagagaatga aaataagtgg aaacttgggt ttttaaaaaa tagtaaagat
    10441 agtattttta agtctaagtt agaagaagtt gtaaaagaag cagagtcatt agatgatgat
    10501 gaatttatag gtcaagagtg ttgtatgata tatgataaag aaacaggtgt tatatcttta
    10561 caaaataata gaaaaagtgt atcatttaag aacgtaagtt cttttataaa tgaatttatt
    10621 gaaaagtctc ataatagaga gaatgtagag ctttatccaa taaccttaaa acctgagtat
    10681 agcaatattt cagacttaga tacaatcaat tatagaagtg tatcattaag ctttttagat
    10741 atgtcagaga ttaaaatgtt agcacaagaa gaatcgaata agtcagttga agcaattgct
    10801 aaaatatcaa ataacttaga ggcacttagt ggaaaaattg aattaagtgt agggtggagt
    10861 aagaatagat ttttagaaaa aataaattta agagaaatcg taagtttttt taaaagacat
    10921 cctaaattaa ctagagggat aaaagtaaaa tcttatgatt ataatagaga tgaaataagg
    10981 gtcatagatt taattgagaa taaactatat gatgaatttc ctataacagt aacaaaaaat
    11041 aaagtaaaaa catttgaaaa aattttttca cctatgtctg agtgttttaa tgatactgta
    11101 gaaaaaactt tggatgattg taataaagtt atggaagaag tatatgttta aatagtaaga
    11161 atatcagaag agagaggagg atgttaaatt atgaaataca aaaaaataat actttttaat
    11221 atttttatta ttatttttat tattttatca aagtatctct cttttgaaaa aaatataatt
    11281 ctctatataa gctttaatac agtaggatat aactttaagg atttattatc aatttctgca
    11341 actatattag ctgtatttat aggatttata gcaactatag caactgtatt agtgtctatg
    11401 tgtgataaaa gaataatggt tataataaaa aggtttggaa aaacaaatat agtgctaagg
    11461 agtataaaaa gagctttatt atcaggtatt atatcattag cattaatagg aattatttat
    11521 gtgaacttag actttaatat aattttatta agatgtatta tcatttgggt tacactaaac
    11581 aatttgttta tttttataag agagagtaaa ttcttggttt ttttagtaaa acatattatg
    11641 gaacaaactt ttttagataa tgaagaaatt gtgtttaaat ctaaaacatc aaaaaaaaat
    11701 taaaatatcc aatcgaaggt tagaactctg aaaagagttc tttttttatt tataaagcaa
    11761 ataaagaggt ggtggtatgg cagatttgac tgaaaaacaa aaaaggttct gtgattatta
    11821 tattgaaact ggtaatgcaa cagaagcata caagaaagca tataaaaata acaatcaaag
    11881 agcatcagaa agcaatggaa gtagactgct gagtaatgat aaggttaaaa attacattga
    11941 tgaaagatta aagcaaattg aatcaaagag gatagcagat gcaaaagaag ttatggagta
    12001 tttaacaaag atactaagaa atcaagaaca agaagaagtt gtaatagtat cagaaaatgg
    12061 tcctgaaata ataaaaaaag atgtaagtat aaaagataga aataaggcag ctgaattatt
    12121 gggtaaaagg tatgctttat ggactgaaaa agttgattta gatggaaatg ttggtgtaac
    12181 tataattgat gatgtaggta atttaaatga tggatagaaa aatatctgaa ataataaata
    12241 aaaattttta tgaattttgg aaagtaagta atagtaacaa atacctatat catgtattaa
    12301 aaggtggaag agcttcagct aagtcaacac atatagcctt ttggttaact atggctatgg
    12361 ttaaatatcc agtaaatact gtttgtttta ggaaagttgg taatacaata atggatagtg
    12421 tatatgagca attgaaagaa actatagaaa tatttggttt aacacattta tttcagttta
    12481 aaaaatcccc aatggaaatt atttttattc caagaggaaa taagtttata tttagagggc
    12541 ttgatgaccc acaaaagata aaatctataa aatcagctaa gtatccaata gcttttgcat
    12601 ggtttgaaga ggttgcagaa ataaaaacag aggatgagtt atctatggta attaattcag
    12661 tgttacgtgg agaattacca gacaaattga attataaaat attcttatca tacaatcctc
    12721 caaaaagaaa acaatcatgg gttaataaga aatttgaaac acatgtatta cctaaaaata
    12781 catatgttca tcatagtatt tatttagata atcctcatat atcaaaggct ttcattgaag
    12841 aagctaatga aattaaaata agaaatgagt ttaaatatag atgggagtac ttaggagagc
    12901 caattggttc tggagtagtc cctttttcta atttagagtt taaaacaatc acaaatgaag
    12961 aaatattcca gtttgacaac ataaggcaag gtaatgactt tggatatgca acagacccta
    13021 tggcatttgt aagactgcat tatgacaaaa agaaaagagt tatatatttt atagatgaaa
    13081 tatttggggt gaaaatgtct ataagagaat tagcttctaa gattaaatca aaaggatatg
    13141 atgactttag tgttatttgt gacagtgcag aaccaagaag tattgcagag cttagagaat
    13201 atggaataaa agcattgaag gctaaaaaag gacctggttc aatcgaattt ggagagaact
    13261 ggctggatga tttacaagca atagtaatag acccaaacag aactccaaat atagctagag
    13321 aatttgaaaa tatagattat caaacagata aggatggaaa tgtaagagct aagttagaag
    13381 ataaagacaa tcattcaatt gatgcaacaa gatatgcatt agagttagat atgaaaacac
    13441 atggaagaga aagaaaatat aacagtagat agggggtgta acatgttaga tttgatagat
    13501 ataattcaga tggaacttac aggattgtat ggtcaagaag taataagaga aatgggtgaa
    13561 attataaggc tatatgataa gtatgaaggt acaggacaac attggataga agaggaacaa
    13621 gattataaac aaacaaggaa gaaaacaaat tatattaaaa agctgattaa agaagaagct
    13681 agatttttat ttggtaagac accaatattt acaataaaac cagagaatga tttagataaa
    13741 gataaggcag aagaaataaa ccagtgtatt aataagattc taaagaaaaa tttgttttca
    13801 gataaactaa ttaaagcggc tagagattgt tttattggta aaagaatagc tataaagcta
    13861 catgcagata aagaaagtaa gagtttaaag ataatgtttg taccaagttt agagtttata
    13921 tatgaaccat ttgataacca atttgatgag ctcaagaaaa tcgtattttt ccatcaaaca
    13981 aatcaagaag ttgaaaaaga taaacagaga atttggaaac aaaaatatga aatggttaat
    14041 ggtaaatgta tattaagtga aggtatttat aatggttatg ggttatgtat agaagaaata
    14101 gtgagtgata cagatttaaa acttagtgga ataccttgtt atgtaattct taatgatggt
    14161 ttgcttggtg atttaaaggg tgagagtgac atagaagaga tatttgataa tcagatggct
    14221 tataataagc tagcttctga ggatatagat actcttaaaa aaggaatgaa tcgtatcata
    14281 tatggtgttg atgttgaaga agagtcaagt aagcatttta aaataaaacc aggagcttat
    14341 tgggatgtag aaacttctca aacggctgac caaaaacaag cacagattaa tacaattcct
    14401 actgactttg gatatgatac tagaatagaa aactctctta atagaattaa atcagatatg
    14461 catgaagtac taaatatacc tcttataaat aatcaggacc ttaaaggtat gatgacatca
    14521 ggtaaatcaa tgaaagcttt gtattggcaa ttaataacta gatgtgagga aaaaatgaaa
    14581 tcatggggtc cagctcttga atggttaata caagcaatga tagagatgat agaagtatat
    14641 aacatagtaa aaatacctat attggacaaa gattcttatg aagtaattgt tgaaaatcaa
    14701 tatccattac aagaagatga agactcagaa aaattacttg atattcaaca agtaaatgct
    14761 caagcaatgt ctaggaagac tttcattaag aagtggaata atagtaatga tgatatagca
    14821 gatgaagaat tgcaacaaat atcaattgaa agacaaatat tagaagaaag ttttaattta
    14881 gaagaggaaa cagaagttat agatacagaa gaatcagaag aagtagttga tgaataatgg
    14941 ctaataagtt taataaggct atgaagaatg ctgaaagagc tagagacata tcatcaaata
    15001 gaactactaa aaagataaga aagttatata aagatatagc taatgaatat gcaaaaaaat
    15061 taaatagagt taactctaac acattaactg aacaatattt aagagaaagt ataatttatt
    15121 taaataaaga gtatgatagg ttaggaaaga agctaaaaaa agatattgaa agtgaaatat
    15181 caaaagtaat aaaaactact acagatgaac aattaagctt tttcaataac atatgtgata
    15241 actattcagt taatttaaag ccacaattca ctgatatgtt tagtaaagtt catgaagatg
    15301 tactaaggca agttatatct ggtagcatgt acaaagataa acttaaatta agtgatagaa
    15361 tttggagtaa tatagataaa actaagaagg atttagacta cattgtaagt agaggattag
    15421 cagaaaaaag aggtagttat gatatagcaa aagatttaga aaagtatgtt aatccaaagg
    15481 ttaagaaaga ttatgattgg tcaaaggtat accctaaaag taataaaaaa atagatttta
    15541 atgcctatag actagcatct acatatataa ctcatgcata tcaaaagaca gctaaagaaa
    15601 gttgtaggaa gaatccattt gttaaaggga ttaaatggat gtcatcacat catcctagga
    15661 tgtgtaaggt ttgtgcagat agaaatggaa aaacatatat tccagaagaa ttaccattag
    15721 aacatccttt aggaaagtgt acttttgaat atgatattcc aatgagtatg gaggacatag
    15781 gtaaagagtt aagaagctgg atagatggag aagaaaactc taaacttgat gaatggtttg
    15841 atgaatatgg attagacttt gcaggaatag aaggaaaagt taataagaat aaagaaccaa
    15901 ctgaagatga gatgttagct ttatataaat atatgggtgg agatgcctat aaaattaatg
    15961 agaaattaag aagaaatatt aaattgacag aagaagatga atggtttata aataacttag
    16021 ataaagtttt agataaaatg ccaaattacg aaggggatgt tacaaggtca ttatattttt
    16081 ataataaaga aagtttaatt gaatttttag agagccatga acttagaaag gttaaagaat
    16141 acaaagaatt tttatcaact acaaagggag aaacttataa ccctaatgca caagtagaaa
    16201 tatatatact tgactctaaa aaaggtaaaa atataagtat gtataatgat aaagaaaaag
    16261 aaattttata tgaaagaaac tctaaatttg aagtaatgga aattgaagaa ataaatggaa
    16321 aatactttat actattggag gaacgagatg agtaaagagg ataaatataa agagcccttt
    16381 agtcatccaa gatggaaaga cattccgtgg ggtgagacta ttagggaagt agagcgaact
    16441 gaggaagaac aagcagaaat aagaaaaaaa tttagaaaga aactaaaaga actaggagta
    16501 ttaaaagaag attaaggcac ttactgaaat taaaaattag taggtgtttt ttattgcctt
    16561 ttttagctat atataggcgt aaaagaaata aatagcaaac tatactagag aagcgaaact
    16621 cgtataaaag cgtagtgtag gagggaataa tggaagaatt attaaaaaaa ttaggatacc
    16681 aagacacaga cataaaaaat ataattgaga gtatgaaaaa agagaaaatc tacacttcta
    16741 aggaagaaaa tatagatgaa agatacaata agttgaaaga acaaaaaata gccttagaag
    16801 agcaaataaa aggtgcaaat gacactatat cagatttgaa gaaaaatagt aaaaacagtg
    16861 aagatataga ggcaaaagtg aaagaatggg aaaataaata taatgaactt gatagtacga
    16921 gtagagctaa aataaggaat atgactatag actatgctat aaattctaaa ctatctggag
    16981 taaatgaaaa atataggaag ctactatgta aagaatttga taccagtaaa atggaagtaa
    17041 aagatactgg agaaattata ggattagatg aacaatttaa agacatatca gaaacatata
    17101 aagaatggtt tgaaagttct actccaagta atacaggttc tccagggaat ttcccaagaa
    17161 aatcaaatgt agtcaataat ccttttataa aagaaacatt caatttaaca gaacaaggaa
    17221 gattattaaa agaaaaccct gataaagcta aagaatttgc agctcaagca ggaataaatt
    17281 tataaggaag agagtgattt aaatggcagt aacaaaatta agtgatgtaa tagtaccaga
    17341 attgtttaac ccatatgtaa taaataaaag catggagtta tcagctctat atcaaagtgg
    17401 aataattaca aatgatgcaa gcttaaatgc tttagcttct caagctgcac cagttgtaaa
    17461 tatgcctttc tttgaggatt taagtggaga atcagaacaa ataatagaag atgctgattt
    17521 aacaccaaat aaaattactt caagtcaaga tgcagcagct atattaagaa gagcaaaaat
    17581 gtgggcagct actgatttat cagcagctat ggcaggaaaa gacccaatgg cagcaatagc
    17641 tagtttagtt ggaagcttct gggctagaga tatgcaaaaa gaacttatag cagttttaaa
    17701 tggtgtattt ttagctgcaa atatgacaac aaataagtta gatatatcag caggaacagg
    17761 aaatgcagca aaatggtcac catcagcttt tatagatgca caacagttat taggagatgc
    17821 acaaggtcag ctaaaggcta tagcaatgca ttcagctaca aaatcagcac ttaaaaaaca
    17881 aaatttaata gaaactatta gaccagatgt aggtccagat tttgaagttt atcaagataa
    17941 attagttata gttgatgatg gttgtcctgt atctaaagaa ggagtttata cttcttatct
    18001 atttggtagt ggggcaatag ctttaggaaa tggtaatcca gtagggtttg ttgctacaga
    18061 aatagataga gataagaaaa aaggttctgg agtagattat ttaatcaata gaaaaacata
    18121 tatactacat ccaagaggta taaaatttac taatgcaagt gtagctaaaa cagaaggtcc
    18181 ttcaagatta gagttagcaa aaggtgaaaa ctggacaaga gtttatgagc ctaagcaaat
    18241 aagaatagtt gaatttaagc ataagttata aaatatagtt tttagtaggt gatagtatgg
    18301 atttagaaat cttaaaaaat ataaaaacag aacttagaga agaacaatca cctttttttt
    18361 ctgatgatga aattacttat tactacaata aaaacaatca agattttaat aaaactatgt
    18421 atgaactatg tatattaaaa gctgaaaatg atagtatcac tttacctgga ggcttaagca
    18481 tgccagaaaa taaactatat tggttaactc tggcaaaaaa gtttaaaaca aatggaagta
    18541 gatgtctatg atagctcaaa aggtaaaacc aaagataatc aaagctatca ataagatgcc
    18601 aacaaaagct acagtaaaaa gagttggagt aaatgagttt ggagagcctt cagatgaaga
    18661 aaatatagtt tgtaatgtga taggtttata taatgaagga agtagctcta taagtcaaat
    18721 aacaaaggat aaaggtattg ttataaaaga taaggagcag tatttaatgg ttgtttgtga
    18781 tgaagataca gtaaagataa agcaaggtga ttttatgtat ttagataata ataagtttat
    18841 tatacaagac cttggaaatc aaaacagaat gaatatttat tttgatttaa agttaggaaa
    18901 ggtgagatag tatgagtaaa ggatttaaat ttgatactag caagctgctt aatgctttag
    18961 taagtagaga aatgaaaact aaggctgcac taggagctta tgcagacact tcatctcaac
    19021 tattagaaag cactgccaaa aacgatagac catggaaaga ccatactcat gatgctaaaa
    19081 ataggataca tggtagttgg gaatggcaag gagacactat aaggatagca cttagtcatg
    19141 gagttgacta tggattatac ttagaaaaag gtacaggtcc acatgttata gaagcaagac
    19201 ctggaagtta tttattttgg gatggagcat cacatcctgt taaaaaagtt aatcatccag
    19261 gaagtagacc atatccaatt ataatgccaa ctatagaaaa atgtggacca agtattataa
    19321 gaggtcttga tgtgatttta aagtaggtga agtatgttta aaaaaatata taaacattta
    19381 aaaactaagg gttttaatgt gtattctatt ggacaacatc aaggattatg catagaacct
    19441 tttttagtta tatttgaaaa aggtcctcta caaacaacag aaaaaaatat aataaaagat
    19501 ttatttgaaa tatatgtatt ttatccaatt ggacagtatt ctaaagtaag tgaatacaag
    19561 ataagtgttg aaagtgtcat ggatgaaata gtaggaataa aacaagctta tgaagcttta
    19621 cctattctta tagatgatga aaaacaagct tatttcacaa gattaagtta ttatgaaaac
    19681 aaacaaatta ggaggtaata aatatggcag tacaaatatt aaatcaatac ccacttacag
    19741 atgttgtatt agttcaaatt gaaacagttg aacaaaatcc ggttacatat acatttgata
    19801 catctgatga gatagggaca gaggaaatca tttctgaagg tgaagaatta actttaaaaa
    19861 taaagaaaaa aataatagca aatagagcag cagaagatac aagtttagga tatgatttaa
    19921 cattaaaaga caatgtattt tgtccagaaa tacttcaaat aatgcaaggt ggaacaatag
    19981 aaaaagaaga agatggtagc tttaagagat atttagctcc agaagtaggg aagacttttt
    20041 ctaagaaatc ttttaaaaca ataatttata gttcagttgt tggaccagga ggagatactg
    20101 ggcaatttgc taagacgact ttcccaaatt gtaaaggaaa atctgttcca ctaaacttta
    20161 aagatggaga atattattca aatgaatatg ttataaatag tagacctaac acagggcaat
    20221 ctccttatga ggttgaaata gtagaggaat tacctaatgg ttatgaagtt acaaaagtat
    20281 ttttagacag tgcatctgta tcaggagcaa cagcaggaga taaaaagata acaggattaa
    20341 cagcaggcaa aatatataag gttacagtta atagcaatat aaaatatact ttagcagatg
    20401 gaacattaac agatactgag tctgataaag cagcattaac aggaacagag ataataggat
    20461 taacaaatgg agaaacatat aaggttgaag aagttagtgt aagtatataa atagatagat
    20521 taaagctcta gtaattagct agagcttttt tagaaagggg acataaatat gaatgaactt
    20581 aaagtaacaa gtttagaaga attgaaaaaa ataaaattga ctgaggtaat agaggttgga
    20641 agatttttag atggaactat gttagttgca gaagtaaaac aacctgactt aatggctctt
    20701 gcaatggctg gtaagatacc aaacagccta atgacagcat caatgagttt agttgaagag
    20761 aaggaaagta aagatagtac aggagaaaaa gtactaaaga agatgaatga tgagtctaat
    20821 ttctcaaaag aaatgtttga aatgatggat atagtggcta aagaagtgct agttaaccct
    20881 acatatgctc aaattaaaaa aataggattg gaactttcta ttgaacaaag attaacatta
    20941 tttaatcgta ttcaaggagg tacaaagtca ctagaaaact ttcatcaaga gtccacagat
    21001 attgaggatt ctaaatcaag tgataatgta caacaagatg cctagtgaga tattaagaat
    21061 taaagatgaa tatacttctt tttgttttga tgaagcttgt atgtttttag tagatgctat
    21121 aaaaaatgat aaaaagctta aatttaaaga aaatagtaat acttctaaag aaaatataaa
    21181 aagaactact tttgttgata tggcattaaa aaagaaagaa aaagatagaa aatagtatta
    21241 atttattcta ttataataga ctaactctgt gatttagtgt aaaatttata atgagaaaat
    21301 aacttcactg ggggattata ttatgaaaat aaacaaaaaa atattatcat tagttctatc
    21361 gttagtaatt gggatttatg cagttggatg cagttctcca agttcgtcag atgattcatc
    21421 taaaaaagat gcaaagacag agcaaaagaa agatgaaaaa aaagagtata aatttggaga
    21481 tactatagaa atgaaagact atgatgaagg aacttacaag ctaacatttg aaggtgtgag
    21541 aagcacagat gaacgtaatg agttcgctga aattgaagct aaaaaagtag tttttgttga
    21601 ttttaactat gaaaatgtag attgtaaaga agctgtattt gtaactgagg gaattgaata
    21661 tcaagtggca gatggtgatg gaaatgtgct acaaacttat ccagtagacg attcaactag
    21721 aatgcaacaa gaaacaacag caggtactaa atcaaaggga acaacagcgt ttgctataac
    21781 atcagactca aatgctgtaa aaataattgt aaaagataaa gtaaataata aggtcatggg
    21841 agaaataaat gctactttag aataaaaata gtattagaga aaagaaaaaa gttaacaggt
    21901 aaaatattct aaatgaatag aataaattaa tgttaattta tgatataatg ataatataga
    21961 tattttgcag tgtacgattt tttatataaa ttggatgtaa tcattgaaag tgctatgata
    22021 taagcatgtt ttgtggtgtg tgaaaaacat tactggttac tcactgcaat tttaatatgg
    22081 ttttatatgt gcaagaactg gaagtgttca atttattttg gggtattata ttaactatgt
    22141 ggtatgtaaa gctgactaca ctctttattt tactagcagc agaagaaagt attatattaa
    22201 ctaagtggta tgtaaaggtc ggacttatta tcatagatat cccttttgcc aaaagtatta
    22261 tattaactat gtggtatgta aaaattgttt taaattgatt aagtacatct gttgatttat
    22321 attatattaa ctatgtatta tacaattaaa ttaaaaaata tatttgagtt agagagggag
    22381 tattttatgt cgttatttgg aagtaaaatt acaaaagaag aaaaagagaa aaatcgagaa
    22441 gaaactgcaa agtattatga aagacttaaa aatatgacaa aagaagaaaa aaaagagtat
    22501 gaaattcaag aatttatgaa gaaatatcag ttagaaaatt tagatgaaaa agatttaata
    22561 gtgttaaaga gaatagcaag tgatttgttt ggaaatggtc ttattaaggc aggtatggct
    22621 ttaagcttta ctgatgcaaa ggaacaagca aaaataggat accttagtgc tttaacagaa
    22681 caaaattgga tgataattag acagttagct cagttgaata ccaatataga aaaacttata
    22741 gaagataaaa aagaataaat tgaaaatagc aatacagaca cttacttaaa tagtaggtgt
    22801 ttttttatat ggaattttga ggaagggagg gaaaaatatg tccttagaat taggaacagc
    22861 agtaggttat ctagatttag atggagggaa gttttttaaa gctcttgcag atgcagctaa
    22921 acaacttagt caatttaatt caagtacaaa aaccacaggt agtgcaatga cagcaatggg
    22981 aaaacaaatt tctgatacag caaattcatt gacacaaata ggtaggcaat cagcattagc
    23041 agcatcagaa ttaaataaga tagcatcaac ttcaagtggt ccattttcat cactaaaaaa
    23101 ctctataaaa cagactgaga atgaactaaa aacagcaaga agtacaattg atgcatatgc
    23161 acaaggtata tctaaactta caagtgaaat agataagtca aagcaaaagt acactgaaat
    23221 aggaaataaa atagaaagat atgagcaaca attagaacgt tgcaatagta tgtatggaga
    23281 aagttcagaa caatcacaaa gatatagaga agctattgaa agacttaaaa attctcaaac
    23341 tcaattaggt aatgaaatcg aaaatggaga agattcttta actgatatga gaacagctat
    23401 gaataatgca gaggcagaag ctaatagact tagtgattca ttaagaagta tgccatttga
    23461 tgctattggt ggcaaaatga gagatattgg tcaaacacta acttctacag ttacaactgg
    23521 actaattgca atgggaacag cagcagtaac agcagctaca aacacagaac aagcaatgtc
    23581 agttgttaac tctatactac aactaaatac agaaaaagtt aagggtggaa aaagtgagtg
    23641 ggatgcctat tcaagtactc tgaaagctgg tgctaatgaa atagggatgg cttatgatga
    23701 atatgctaat gctgcatata atgctatatc tgcaagtgta aaacaagcag atgtaaccga
    23761 attcttagca caagcagata aactagcaac agcaggatta actgatttag caaaagctac
    23821 agatgtgttg acaactgttc aaaatgccta tggaatgtct cagaaggata tggctcatgt
    23881 aagtgatgtt cttatacaga cccaaaataa aggcaaaatt acggtggatg agttagcaag
    23941 ttctatgggt aaaataatac caacagcaaa aagtttgaat gtatcagttg agcaactagg
    24001 agctggatat gccatattaa ctgcaaaagg aattgctggc gcagaagcaa caacttacat
    24061 gaatgcaatg ttcaatgaac ttgggaaaag cgggagtaaa gcagataaaa tacttaaaaa
    24121 attaacagat aaaggatttg ctgatttgca aaaagaagga aaatcaacag cagatgtatt
    24181 attaatatta aatgattatg caaagaaaaa tagcttatct ctatcagacc tatttggctc
    24241 ggcagaaggt gcaaaggcag ctaatgtttt actaggtgat gcagttaatg aaactacagg
    24301 agaaataatt gaagggacaa agagtgctga ttcatttaat caaatgctgg gagatatgaa
    24361 aaattctact ggactttgtg ataaagcgtt tgagcaactg gataatacta caaagactaa
    24421 acttgaagat gcactcaatt cagcaaagaa tatgttatct gacttaggtg aagttatgat
    24481 gcctgtagtt gctaaagtgg ctgaaatggc aactgctttt atgaaaagtg tcagcgaaat
    24541 ggcaaagagt aatcctagtt ttactagaat cgttgtaagt attggagctg tagtctcagc
    24601 aataggacct ttattagtag tccttgggac aatggcaatc acaataccta aggccattgc
    24661 tgcaatcggt acagcaggaa aagcatttac atttctatcc acaagttttg ctattgcaag
    24721 agaaataata acacctgcaa gtgtaggagc aagtgcagga atgataaaaa tgtctaaggc
    24781 tatgacagca ttaatgggac ccatgggttt agtagtagct gggatagtag ctttaggagt
    24841 ggcttttgta gctttaaata aacacatgtc acaagactca attcaagttt cagaaaggtt
    24901 tggaaaaaac atatcagatg ggacacaaaa agcagttgga gcttttatgg atttagaaga
    24961 gcaaacaaca actactttaa atcagatgtt gtggtctgga gaaactgttt caactgaaat
    25021 gaaggatact ttaataagta attttgactc tatgtctagt caaataacat caaagttaaa
    25081 tgaaacaaaa gaaagcagta aagcaacatt acaagaaatg tttgttagct caaccacttt
    25141 atcagacaaa gaaaaagaag ctttaataaa aaataccgat gaagcttata gcaacaaaga
    25201 aaagaaaata gaagaaagtg cagctagagt aaaagaaatt ttaactaaag caagtgaaga
    25261 aaatagaagt ctgacacaat ctgaagcaga tgaaataaat cgtattaaaa cagaaatgtt
    25321 aaatacagct gtaaatgtca tgtctgaaag tgaagcagag caagcagcga taatggaaag
    25381 aatgagagtt aatgctacaa atctctcagc aaaacaagca gcagatgttg tgaaaaattc
    25441 tataaagcaa agagatgaaa ctgtaaaatc agcaaatgaa gagtacaatg agagactcaa
    25501 gttagcagct caacttagag ctgagggtgg aaaagaaaat gaagagctcg caaataaagt
    25561 aattcaagaa gctacaaaac aaaaagatga agcagtaaaa aaagcacaag aaatgcacaa
    25621 agaaattgtt tcagaagctc aaaaacaagc agaaggtcaa gtggaaaaaa ttgattgggc
    25681 gacgggagaa ataaaatcga gatgggaagt attaagagat gaaatcaaga agaatattga
    25741 tacatctcaa agcgatttcg acacttggtg tagtaataca gataaaggat ttgctgatac
    25801 gtggaacaaa ataactcaag gagtttcaaa tgcttggaat ggtataacag agagtttgtc
    25861 aaatgcgtgg aatagtataa aggaaggtgt tagtagtgct tggaatagtg ttttggaatg
    25921 gacaacaaat acttggagta atataacaag ttttttaggc gaattatggg aaagtattaa
    25981 agaaatggct ataaatgcat ggaatggtat tgttagttca gtaacagatg catggaatag
    26041 tataacagaa ttcttaagtc cagttacctc attcattagc gaaatggtaa aaatggcatg
    26101 ggaatcaatt aaagaaacag ctataactat ttggcagtct atttctgata taatacaaag
    26161 tgtttgggat tttataaagt cgattgtggg tcttggcgtg gaaatagtaa aagcattaat
    26221 aagagctgcg tggcaaggca taaaagatat aacttcatcg atatgggaag gcataaagaa
    26281 tgtaatacaa agcgtttggg atgcaataaa accatatgta atgggtgcag tagatgctgt
    26341 aaaaacagtt gtaacaaatg catggaatag tataaaggat atatcaagta caatttggaa
    26401 tggaattaag acagttgtaa caaacgcatg gaatggaata aaatcaattg ctagtagtgt
    26461 gtcgaatgca gtcaagtcgg tagtgtcaag tgcatggaat ggaataaaaa gtataacttc
    26521 atctatatgg aatggaatca aaacagcagt atcaaatgca tggaataaca ttaaatcaag
    26581 tgctacaagc tcagtatcaa atgtaaaaaa tacaacaaca gcagggttta attccttaaa
    26641 atcagcggtc acaactatat ggaataatat aaaaacagca atatcaaatg tatggagtaa
    26701 tataaaaaca gcagtaagta atggtgcaaa tgcagtaaaa tcagtatgtt caagtgtatt
    26761 tagtacaata tctaatattt tgacagctcc atttaaaact gcacaaaaag caataagtgg
    26821 tatacttgga ggaattacaa gtaaaataaa tggaataaaa gataaagtaa gtggactttt
    26881 tagaagtggt cctataattc ctcaaaatag taacgatttt attcaaaaag atatgttacc
    26941 aacaccaatg atgtttagag tatcagttgc aggttttgca tcaagtagag gaagtatact
    27001 agactctata aacaaatttt ctaatgctat gacaaaaggt atgggtagtt cttcatcagg
    27061 aatagcctca caatttaaaa aggcaggaga aaagtcagca aaagcttatt tagatggatt
    27121 gtcaaacata gaacaaggat taacaaatac acttagaaca gtccaagcaa taataggtaa
    27181 tggaaacata aaagaagcac aagaaattaa aaagtttaat aaggaagttc aaaagctaca
    27241 agaagaaaaa gctgaagaaa tagcagaatt agataaagaa cataagaaga aacaaaaaga
    27301 aagaaatgaa aaagaaaatg aagaaataaa agaagcagaa gcaaagaaat ataagaataa
    27361 aaaagaaaaa ctagcagcac taaagaagat taaagataaa tataataagg aatctgtaaa
    27421 agaccaagaa gaatacagag aaaagttgga aaaaattaat aaaaagtata ataaaaaaga
    27481 aaaagaggat actgaaaagc atcaagaaga actacaaaaa cttagagaag aaaaaataaa
    27541 agctgaaaga gatttcaata aaaaatataa taatattaaa gaagattatg ctgaaaaagt
    27601 agcaaactta gataagaaat atattgagga tcaaaaaaaa ctcaatgaag agtatcaaaa
    27661 gatatatgac tcaagagtaa aatctttaat ggattataca gacttatttt cttctgtaac
    27721 atttgaagaa attgacaatg atgagttaat ggaaaaccta gaagaacaag ttgatgtact
    27781 tagaaaatgg gacactgata tggcagattt atctacaaaa gtaggtaaag atttatatga
    27841 ggaactatta gctaaaggac cacaagccca taatgaaata gaagctatta acaagatgtc
    27901 aaaagagcaa ctagaagaat acgaaagatt atatcaagaa aagaagaaaa tagctgaaag
    27961 aagagccaaa gaagatacag aagatgaaaa atatagaata gaaaaagaaa ttgaagaatt
    28021 gaaaaaaact tatgatgcag agtatagaaa actaggtcaa gaaatgcaaa gaagtataga
    28081 ggaacaaatt gaaagtttta aggataaatt tggagtagta ccagctatgt ttatggaaac
    28141 aggcaaagat tctatgcagg gtatgataga tggtattaaa tctatgcaag gagcgttggg
    28201 caaggtttta agtgacatag ttaattctat aaataaacaa ctttctaata tagggtttaa
    28261 agatgtaaat atacctacag gaggaagaaa agaagcagca aggtttagaa atgaaataga
    28321 aaatttacaa agtatagcat attcagatac tctggcaaga ggaatagcat caaataattt
    28381 actgaaggat gcaactgtta atataaataa taatagtaaa gttgatagtg aaaaaagtag
    28441 tgataagaaa gttgagttaa ctttacatat agaaaagttt ataaataata ctaaacaaga
    28501 tatagagcaa ttaggttctg aaatagcatt tattacaaat agaaaactaa agttttaatg
    28561 tgataatatc ttttcaatat ccaaattgtg gtataatact tttagcaaga agatgtaacc
    28621 tataatctaa gagtggagtt catactgaga taaaacctac tttctaatga aaggaggtgg
    28681 taagtatgaa caacttttta cttaatgtaa tagctggtgt tattgctagc ttaatatttt
    28741 gcttaataag taagatattt gcaaaagtaa aaagccactc tgtgcgaaag agtggctggg
    28801 agtttgattt aaaaatcaaa ttccataaaa aataattagc ttttaattat gaactccgct
    28861 ctactgacaa tagattgtag ttcttcttgc ttttattata tcacatttta gcaaaaatat
    28921 gcaaagtaaa tagaaggaga tgtatcaaaa gaaaaatttg agtcctctct ttttttatgt
    28981 gtaggaggtg ataatttggc tttacaatac tgtgtcagtg aaaatgaatt agtttctttt
    29041 caatatggcg aaataaatag tagagatttt ggtattgtaa ttacagatat taatgaactt
    29101 gcatcgccag aacgtagatt tgaaagaata gagataccag gaaggaatgg gagccttatt
    29161 ttagatgaag gttgttatag taattttaat cttgaaatag aatgctatat agatgtagat
    29221 ggtaaagata ttaatatact agcatctgaa ataaagatgt ggttacaaac tgatttttca
    29281 tataaaaatc ttattataag tactgaccca aactattata gagaagcagt ttgtattaac
    29341 aaactagatt tagaagaaat tataaaagat ttaggatatt ttaagattac atttgaatgt
    29401 aaaccactta agaaagaatt agttaataat ttaattacaa taaatgaatc agattctaag
    29461 ctaataaaca ctggaatggc ttcgtatcct cttataaaag tatttggaaa tggagatata
    29521 acattagaaa taaataatga ggtgatagaa cttacagaag ttcaagggca tatatatatt
    29581 gattgtgagc ttatgaatgc atataaaata gatattcata caaatggtgt agttaatgaa
    29641 aatagtagga tgtttggaga ttttccaaca tttaaacatg gtgaaaatat aataacatac
    29701 tctggagatg tagaaaaaat agagatagaa cctagatggg tggtgttata gattgcttag
    29761 attatataaa gcaaatgaaa caaattttaa aaataatggt ttggggattc taaaagatgc
    29821 atttgaaaca aaagttacag aagaattaaa tggactattt gagttagagt ttacatatta
    29881 tgtgggctct tttttatttg atgaaattga ttataataaa atagttatgg ctgatgcttc
    29941 tccaaggctt aaaaatcaat tatttaggat ttactatatt tctaaggaac ttgatggaaa
    30001 aatattagtt aaagcagaac acattagtta tgatttatta aataatttta tagaaaattt
    30061 agaacttaaa aatgttacct gtgaagaagc tcttaatcaa atatttaggt catgtactga
    30121 agaaaataga tttgtaggat attcagatat aacaggaaat aaagactttt ccatctcttg
    30181 tgtaagccca cataatgcta tatctgacat acaagaatta tttaataata agtctaaact
    30241 aaaaagagat aatttcaata taagtttatt aaataatatt ggagaaagta acaatgtatt
    30301 actagcatat aggaagaata taataggtct aacagctact tatgatacac aagaagttat
    30361 aactaagata tatccatatg caactaaaaa aagtggtaaa aataaaaaaa ttacactacc
    30421 agaaaaatat atagaaagca agtatataaa caattatcca actcaaagaa ttgttgcagt
    30481 tgatttttca gatgatgatg ttaagaatga agaaagctta aggaataaat gtaaagacta
    30541 ttttattgaa aataatgttg atttacctaa agttacttac aaagtagaat ttgtagattt
    30601 atcaactaca gaagattata aaagctataa gatgcttgaa actgtaaata tggatgatga
    30661 gataatagtt agagactata acttaggtat aaatgctact gcaagggtag taaaaactga
    30721 atataatcca gtattaaaga aatacaattc ggttgaaatt ggtgacttag taaatcattt
    30781 taaagacgaa agaatagatg atttagaaga aaaaatagat aaggttcaaa ataatgtaga
    30841 taatattgta attgaaagtg ataattttcc ggatacactt ccagagcctt caaatgtcac
    30901 agcattaggg ttatggagta tgattcagtt agattggaca tttgataata agttatatta
    30961 taactatgaa gtatatgcat ctcagataaa aggatttgaa ccagatacta taggatatac
    31021 aaataggata tatgtaggtc aagcaagttc attactccat gaagttaagc ctatgcaaac
    31081 ttggtattat agagtacggg caggaaatac acatgataac tataatgaat tttcaaatga
    31141 agttagtgca acaactagaa agttaagtga tgcagctgaa tattttgaag aagcagctat
    31201 aggtcatgca gtgataagag atttagatgc agataagata aatgtaggaa aagtaaaagg
    31261 gcagtatatt gaggcaaaga atttagttgt agttgatgga aatagccaaa caactttaaa
    31321 tatagatagt tttggaaatg ttcatatagg agcaactacc tttacattaa aaggaaagtc
    31381 gttggaatct attattggcg gagaaataga cgatataact caacttgaga tatttaataa
    31441 actaactaac aatggtcttg caaagggact ctatatggtt gggaatgaat tatatctaaa
    31501 tgctagttat ataaaaactg gtactttaga aggtcaattt ataaatggta gaaatttaac
    31561 tgtcagagat aatgatggat atacaacgtt acaagtagat agtaatggaa aagtgaatat
    31621 aagagctaat gagttatcta taggtgataa aaataactat gaaagtgttt taactagtga
    31681 ccaaaaagca gtatttgatg cattaacagg gaatcgaaat tgtggaatat atttaagtgg
    31741 aagtagatta tatataaatg ctgattatat agacacaggt actattttat gtgacagaat
    31801 aggtgctagt tcatcaaacc cattcatatt actatttgag gggaatggtg caaaatgtgc
    31861 tttagatgct actgctcagt ttggagtagg tataggaaaa gcaatgcgta tgaaatataa
    31921 tgattattca tatatatatg tttctgatga tgtaattagt ggttatcttg atggagagga
    31981 agtatttgaa tttggttatc aagatgaaaa aagttacatt aatacaggtt tgaatacaca
    32041 acatataaat cctcaaatag atggttggta taattgtggt tcaaatagga aagcttggga
    32101 ttatttagta tgtaataatt taaatcaact agcaagaaca gcagctacat ctactcgaat
    32161 gatgaagagt acatttaatg aagaaatttc aaataactgt atagattttg ttaaaagtag
    32221 cttagtacaa tctgatgtat tcacaccata taatttaaaa tctatgaaaa acaacaatat
    32281 tgaacataga ctacaggttg atatagactc ctctttagat aatcctattt ctaagtacat
    32341 tttcaaagat gtatcagatg aagctggtga aggtgtctat gcacaagata taacatctca
    32401 tttagcagta ttacaattat ctttacaaaa aactatatca aattttgaaa attataaaga
    32461 tataacaagt aaaaaaatag aagaacttac aaaacgaata gaagtattag aaagcaggtg
    32521 atataacttg agagatagaa tttatattgt agatatcaac acaaagatgt atcaaatagc
    32581 aagatataag caaaatgata ataatgtatc ttataacatg agaatagtac aagactctat
    32641 agatgttgat ttaactggat atacagcatt agcatttttt aatttaccaa gtggaagagt
    32701 tactcagaaa aattgtacta tagaagattc aactgtttat actgaattaa gtcatataga
    32761 gctttcagaa gaaggagatg ttatttctga gataacttta tataaagacg ataaggtagt
    32821 tactacgttt tctacaataa taaaagttga aaaaagtata aatagaaatg caatagagga
    32881 tgagcctagt tgggatataa taaaggacat attaaatgta ttaagttatg aagaagaaag
    32941 acaagaaaat gaaaatgtaa gaaaatctaa tgaagaaatt agaataagtt ctgaaaatgt
    33001 tagaattgga aatgagaatg taagaattga aagcgaaaat cagagaaaag atagtgaagt
    33061 tgaaagatgt gagagtgaag aagtaagaaa aacacaggaa gttactagag aagctaatga
    33121 agaaacaaga aaaacaagtg aaacaacaag agaagctaat gaggaaataa gaaagacaag
    33181 tgaaataaca agagaagcta atgaagaaat aagaaaaaca aatgaggtag acagagagtc
    33241 aagtgaagaa actagaaata ctaatgaaga aacaagagaa gaaaatgaga ctgttagaga
    33301 atcaaatgag gaaataagaa aaactagtga agaaactaga gaatcaaatg aaaatacgag
    33361 aaaagcaagt gaagagataa gaaatggtag cgaagaaaca agagttgcta atgaagtagc
    33421 tagacaagaa tcagaaacta caagagaaga aaatgaagac cttagaaaaa gtaatgaaga
    33481 aacaagagaa gaaaatgaag aaataagaaa aagtaatgaa aatactagaa aagcaagtga
    33541 agaaataaga aacactagtg aagaaacaag aaatactaat gaagaagcta gaaaagcaag
    33601 tgaagaaata agaaaaacta gtgaaacaac tagaaatacc aatgaggaag ctagaaaagt
    33661 tagtgaaaca acaagggaaa caaatgaaga aacaagaaaa acaaatgaaa taataagaca
    33721 aaattcagag actaaacgaa aagagaatga agagcttaga gaaagtaatg agggaactag
    33781 agaactaaat gaatcaacta ggcagacatc tataattaat atgcagaaaa aagtggatga
    33841 taaagtagat gaggctaata acaaaatagt tgaagtaaat actgctaaga cagatatgac
    33901 aacaactgtt agcaataaaa taactgagtt tgaaaaaagg ttcaatgaat tagaaaattt
    33961 agatacagca ggagaaatat tacaatcaaa agaaacaaca gatggtcaga taaaagatac
    34021 tttaaaagag agattaactt atgattttaa taaactagat ttaaagatag agcaacttat
    34081 tgctggtgga ataaatgtag catttaataa atcatatgat acatctgaat ggattgaagt
    34141 tgatggagga tttgaacttt cagtagaaca taaccttgtt actcaaaaaa tattagtctc
    34201 ttcaatagat aaacttaata ggaaaagttt gattacatct tacaaaatta tagatgataa
    34261 taatatagta ctatttaatg aagttgctat agatattgaa gttacagtag ttaatggtgg
    34321 ttctgctatt gaacttataa aatttactat taatgatgat atagagtctc ttgaaagtgc
    34381 ttattctagt atagagacaa aaagattatt aaaagatgga ttagataaaa aagccaattt
    34441 agaacatact catgatatat atgttgagaa agttgaaggc aaagaactct ctacagaaga
    34501 ttatacaact gaagaaaaag aaaaactttc tcaattagat acttataaaa ataaagaaac
    34561 aaaatttaat acaaatggtt ctataataga aatattagat aataatatca agtatattac
    34621 aaagtttaat acaaatggtt ctatatcaaa agaaaagtat ataaatgata tattaataaa
    34681 tacaacaata actaacttta aaaatggaat gatagaagaa actgttacaa atgaaggggt
    34741 gatttagtga gttgggcaga ggtttataaa ataaatagtg atattcaagg agaaccttta
    34801 aattttttaa attatttaca agatttgaag ttaaatggtt ctgaaagtta tcttttatat
    34861 gaaggtaatc ataggatttg ggaagagatg tacttaaata gtttatattt attttctgat
    34921 agaggaatta gagaagttgt gtatacagct ttttcagaaa cagacattga taatctgttt
    34981 gataaaggta ctaagctggg agaacaatta aatgcctttt acagaacaga tatatttagt
    35041 ttgggaaatg ctgataatgt agtaaaagga atgactgtag agcattataa ttcattagag
    35101 gaaaaattta aagcagggta tgatagatat gttacaaggg aacaagaaaa gtcaactata
    35161 ggagcatggt ttaattctac ttttagttta aataatactg gtttagaaag tcttacaacc
    35221 atagaagaaa tattagcaaa tgtggaagtt actaatgcaa tacttaataa tagtaatgca
    35281 atagtagctt taactatgtg taaaacaagt atggacgcag ttgttgcaag cccaaatgca
    35341 atggatttgc taggacaata tattttaaaa gttacagcag agccagctgt tataaaagcg
    35401 attttagaaa ataatgtaat tagagaagct ataataaata gtactgaagc aatgacttat
    35461 atatcaacaa atcaggaatc tgtatcatat atatttaaaa atttagaagc gatacaatta
    35521 ttactagcta atcaagaaag tctaaattta gtattagcaa atcagaatgt agtaaaaaat
    35581 atattagcaa atattttaaa aacatataat gctttgtaca atattgagtc aaataaaaca
    35641 aatttaattt caaattcaaa tactgcttat aattcaacaa atcttatagt tgagggagaa
    35701 cttttctatc tgataaaaga tataaacatt gaatataaaa gtttgaagga agctttagta
    35761 gtaattggag aagatacagt aacagtactt aatatttgta aaagtataga aaataatcta
    35821 agtattataa cagctatagc aaataatcaa gaagctttca attctgtaaa agaagttgac
    35881 ttagcaatat caaaaatagt agcgaagctg gcaggtttag ataatgcaac tatctacact
    35941 tcggatgcgt tagctagaag tgataaata
//
LOCUS       DAELJO010000015        35439 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELJO010000015 DAELJO010000000
VERSION     DAELJO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.015"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(203..1639)
                     /locus_tag="KPB88_000711"
     CDS             complement(203..1639)
                     /locus_tag="KPB88_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG0053015.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(1710..3092)
                     /locus_tag="KPB88_000712"
     CDS             complement(1710..3092)
                     /locus_tag="KPB88_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0053016.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMCGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(3228..4133)
                     /gene="garR"
                     /locus_tag="KPB88_000713"
     CDS             complement(3228..4133)
                     /gene="garR"
                     /locus_tag="KPB88_000713"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG0053017.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            4529..5200
                     /locus_tag="KPB88_000714"
     CDS             4529..5200
                     /locus_tag="KPB88_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0053018.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            5303..5851
                     /locus_tag="KPB88_000715"
     CDS             5303..5851
                     /locus_tag="KPB88_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053019.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            6113..6421
                     /locus_tag="KPB88_000716"
     CDS             6113..6421
                     /locus_tag="KPB88_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053020.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(6583..8373)
                     /locus_tag="KPB88_000717"
     CDS             complement(6583..8373)
                     /locus_tag="KPB88_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053021.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(8373..10100)
                     /locus_tag="KPB88_000718"
     CDS             complement(8373..10100)
                     /locus_tag="KPB88_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053022.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(10345..10611)
                     /locus_tag="KPB88_000719"
                     /pseudo
     CDS             complement(10345..10611)
                     /locus_tag="KPB88_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            10908..12020
                     /locus_tag="KPB88_000720"
     CDS             10908..12020
                     /locus_tag="KPB88_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0053023.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(12384..12893)
                     /locus_tag="KPB88_000721"
     CDS             complement(12384..12893)
                     /locus_tag="KPB88_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0053024.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(12895..13542)
                     /locus_tag="KPB88_000722"
     CDS             complement(12895..13542)
                     /locus_tag="KPB88_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053025.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(13556..14728)
                     /locus_tag="KPB88_000723"
     CDS             complement(13556..14728)
                     /locus_tag="KPB88_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0053026.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            14996..16372
                     /locus_tag="KPB88_000724"
     CDS             14996..16372
                     /locus_tag="KPB88_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053027.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSDYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(16622..16981)
                     /locus_tag="KPB88_000725"
                     /pseudo
     CDS             complement(16622..16981)
                     /locus_tag="KPB88_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(17308..17838)
                     /locus_tag="KPB88_000726"
     CDS             complement(17308..17838)
                     /locus_tag="KPB88_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0053028.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIKEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(17926..19020)
                     /locus_tag="KPB88_000727"
     CDS             complement(17926..19020)
                     /locus_tag="KPB88_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG0053029.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(19022..19468)
                     /locus_tag="KPB88_000728"
     CDS             complement(19022..19468)
                     /locus_tag="KPB88_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0053030.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            19774..20964
                     /locus_tag="KPB88_000729"
     CDS             19774..20964
                     /locus_tag="KPB88_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0053031.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            21134..22096
                     /locus_tag="KPB88_000730"
     CDS             21134..22096
                     /locus_tag="KPB88_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0053032.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(22218..22880)
                     /gene="zmp1"
                     /locus_tag="KPB88_000731"
     CDS             complement(22218..22880)
                     /gene="zmp1"
                     /locus_tag="KPB88_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG0053033.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(22980..23065)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(23250..26168)
                     /locus_tag="KPB88_000732"
     CDS             complement(23250..26168)
                     /locus_tag="KPB88_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0053034.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKKPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPMVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(26286..26371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(26498..27676)
                     /locus_tag="KPB88_000733"
     CDS             complement(26498..27676)
                     /locus_tag="KPB88_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0053035.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            28051..28200
                     /locus_tag="KPB88_000734"
     CDS             28051..28200
                     /locus_tag="KPB88_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0053036.1"
                     /translation="MFFISYIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(28301..31075)
                     /locus_tag="KPB88_000735"
     CDS             complement(28301..31075)
                     /locus_tag="KPB88_000735"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0053037.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(31247..32503)
                     /locus_tag="KPB88_000736"
     CDS             complement(31247..32503)
                     /locus_tag="KPB88_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0053038.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(32575..34077)
                     /locus_tag="KPB88_000737"
     CDS             complement(32575..34077)
                     /locus_tag="KPB88_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0053039.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(34168..35358)
                     /locus_tag="KPB88_000738"
     CDS             complement(34168..35358)
                     /locus_tag="KPB88_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053040.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMHSIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT        11723 a         6020 c         4197 g        13499 t
ORIGIN      
        1 attaaaaaag ataaataata aaataaacac ataagttaaa taataaaaat actaatacat
       61 aagattaagg tttagaatat ttttgtaaaa tactctaaac cctaattaat atactattat
      121 ttttaatctt aatttataat tttagtttat tttaatttta actcagtaaa tttaagtgtt
      181 taaacttcaa tacactaata tactacccca tcaatatctc aactgattct ctaagtgtgc
      241 taatctctcc cacatttcca gggaaaatta tatatggcat attaggaaat ttgctttctt
      301 tgcctgtcat ccatactggt ataccaggtt ttatctgtcc cattactgta gcttttttta
      361 ctcttagtgc tttagtacca acatcactag aagtaattcc acccttagct ataataaaat
      421 ttggtcttac attaagcata ccaattatac tggtaactgc atctgatatt tctacagaaa
      481 tcataagctg tttatcttta tcattcgtat ccaagtcaaa acgctctcgt ctagtataaa
      541 cagcaacagt tttaccactg cttatttttt cctctacaac ttttataact ctttttacct
      601 cattttttag accattttct tgaagaacta aatgttgatt aaattctata aattctattg
      661 gatatttaca atttttaagt tcttctaact gttgggtagt cttattcaca tgtgaaccaa
      721 caagtataat tcctccattg gtattacctt ttgatactaa ttcatcctta gtaagtagtt
      781 ccttgtcact cactccgcca agtacttttg ttatggcagc agcgcttcta aacataaatt
      841 ccttaccact atttactgct cgtataaacg caattgtaaa tacctttaca tctacatagt
      901 cggttgcatt tacaataatt ttattgaatc cttcagcttt tttaagtttt tctgttatag
      961 cttcaatatt tagctctcta agttcttttg acgaaatata tatcatatca ctagatttgt
     1021 actttccatt tgtcttttct tcacaccact cacctatatc agatgattta tatccaaaag
     1081 atttatcttt tgcaaattca gtcattcctg ctggtacaag tatttctcct tctttaacat
     1141 aatgtacatt atctatagta aacctaccac cttctttaaa aaatggcatt attatttcac
     1201 cctgaaataa cttccccgat aatctctcta cttcgctttt taataaatta gtttcaagag
     1261 gataatgtcc tctcatagta gaatcactac gacttatgat tataaaatcc ttattaactt
     1321 tctttgatac atccacaata ttctttgaaa tttctttatg tgcttttgta gtttgagcaa
     1381 ctgtaaatcc ccttgaatta gtaagtataa aaaacatact atttttctca ttaaaacctt
     1441 gttccatact atccttatcc caatcagtat aaacagaaat atcatgaact gtttgtacac
     1501 ctgttgggtc atcatctaat acaacaattt ttctatctaa atgcttaagc tcatctgcaa
     1561 gaagttcatt taatctgtcc tcatctactt gtttaaaagt attaagtata tctatgctaa
     1621 ttggttttac ttctctcatt taaaacgccc ctttatgtaa tttgtatagt ctaaatgtaa
     1681 tttatatgat ataaacaata gtttgttatt tatagacctg gaagtggtat aattccattt
     1741 ataacactaa gcaataaaac cattataaat gctacaactc cagcaatggt tcctccaatt
     1801 gtccaagagt ttaaagtatc tttaactgat aatccactaa atcttgaaag tacccagaat
     1861 ccagaatcat ttggaaggga gaaaccaact cctccagcac atatagcaag ccctactaaa
     1921 actggtgaca ttccagttgc caaaattgta ggtcctaaaa ttgatgatgt tgttactaag
     1981 gctacagtag cagaaccttg agaaattcta agcaaggcac ttaataagaa tccaagcact
     2041 acaactgata tactaagacc agacatagtg tcaactaaga agttacctat accacttgca
     2101 tttataacac tgccaaacgc accacctgac cctgttatca ataatattag ccctgctgaa
     2161 tcagcagctt caataactac atctcccata ggtttattta tgtatttctt taaaaatcct
     2221 attgccacca atattccaag aagtattgct atgtttttat ctcctataaa tgcaagtact
     2281 ccacacattg tagaaccttt ttctaagaac atactaaata catttgatat aagtataaga
     2341 agtattggga acaataaaga aaacatagaa attccaaaac ctggtacttt agaagaatca
     2401 tcatcttcaa tttttatgct atcttttccc tgctctaaat ttttttctct atcattctca
     2461 tcaatagcat aagctggata tttttcaaaa cgttttccat aaatccatcc acctataaga
     2521 gctgctggaa aagcaacaat tatagaataa agtataaaac tacctacatt tgcatttaca
     2581 tttgctgcaa ctgccaaagg tcctggtgta ggtattacaa gtgcatgacc tactatagtc
     2641 ccaactccta aggcacatac aaaaaccatt agagatttct tagttactct cgaaacatac
     2701 ttaattatag gcatcattat aacaaaagct gcatccataa aaactggaat tgaaattaaa
     2761 aaacctgctg tagttactgc taaagcactg tttttttctc cagtcttagc taacaacccc
     2821 tttgcaatag attctgttgc tcttgattct gaaagaatat ttccaaatat tattccaagt
     2881 ccaattacaa tacctatact acctaatgta tttccaaatc cagtcgatat tgtagttgat
     2941 atttcattta taggcattct aaccataaag cctgtaatta tagatgttat cagtaatgcc
     3001 aaaaacggat ttactttaaa tttaataatc gatacaagta aaattcctat agatataaca
     3061 aatgctatca aaagtccata tcctgtaatc ataatcttta cccctttatc attctacttt
     3121 attatttatc aattttattt ttcttttact atgataactt aacctcgctg tatttatata
     3181 atctaatcct aataatatgt atttaattat attgatatat ttaaatatta attatcgtgt
     3241 ttttttattt caacacctgc caacttttca aaatactgtg ctattccact atggtcatct
     3301 tccaaatgtc catgtacttt taatgtctgc ataatttcta ataattgact tgtcataggt
     3361 agtggtacat ctaaattgtg tgctgtttgc attacattct taatatcttt caaattaata
     3421 gatatttttc cacctggttt aaaatcacga ttcataatca ttggtatctt cacatccaat
     3481 attgtactac cagctaggcc tcctcttatt gccttatata ctttttctgg gtctgcacct
     3541 gcttttgctg caagtacaaa tgcctcagaa actgctgcta tcgtcaaatt aactattact
     3601 tggtttgtaa gttttgtaac acttccacta ccattatctc caattaaaat agatgagctt
     3661 cccattatat taaaaaatgg ctctactttc ttataaattt cttccttacc accagccata
     3721 aaagcaagtg taccatcaat cgcttttggc tcccctccac ttactggtga gtctataaat
     3781 tctaatccca tatcttttaa tttatttgca cacaatatgg attctgtagg agtaactgaa
     3841 ctcatatcga ctactatact tccttctttt aaagtttttg ccaatccatc catcccaaac
     3901 aatatatcct gcacaatagt tccatttgga aggattgtaa atacaatatc acattcttga
     3961 cccatttcta aaacacttgc gtattttgct ccacatgata taagttcatc cacagcagat
     4021 ttattaatgt catacaccaa aagattacat ccatctttta ataaattttt tgccattggc
     4081 tttcccatta ttcctaatcc aataaatccc aatttcattt tataaccctc catatcttca
     4141 catttttgaa aacgtttttt atgttttaga gtatactaca taccagtata ccggtcaata
     4201 taaaatataa aagtatttat tttaacaata aatagtaatt tttaaagtca tttattatat
     4261 ttagtcatcc tatataagtt ttaatacata ctattgtttt caaatataat atactacttt
     4321 tacagttttg tattttaact aatttcaatt agttttaatt aatctgcctt taatgttaat
     4381 catgcttcac acttcaataa ttttttatat tttttaatct tatctgcaat ttaaattgca
     4441 aaagattgac cttattttca ctatgttgta tgcttaaaat tatataagtt tttgtatttt
     4501 tatattgttt aggggggtat tattaagtat ggctacattt aaaagattac aagaacaagc
     4561 atatgactac ttaaaagaat tgatactatc tggaaaaatg atagaaaatg aaatatattc
     4621 tgaaacaaaa cttgctagtg aaataggtat ctctagaaca cctataagag atgctcttca
     4681 aagacttagt caagatggat ttatagatat aattccaagc aaaggattta gaatccatca
     4741 aattacagca aatgaaattg tagagatatt ccaaatacgc tctgcaatag aagggttttg
     4801 tactttttta attacaagtc aatacaaaga acctagagca gtagaaacta taagtaaatt
     4861 aaaacatctt ttagataaac aaaaagatat tttattaggt gataaaaatt taaactcatt
     4921 tgcagaatac gatacattat ttcacacaac tatagtttca tatgctcaaa atactgaatt
     4981 tgacaaaatg tttaacaatt acatgtacag aataaaaaaa ttagctctcg attcattgtc
     5041 acatgaagga aggcttgaaa caacattaaa agagcacaca gatattttta ataatattgc
     5101 aaatggatgt attgaagata tttacagaac tactctcatt catatggaaa ctcctaaata
     5161 cattaattta gaagattttt gtaacaataa gttattttag tattatactt tcatttatta
     5221 aaaatatatc tatctcaaat taatttccta atttataaaa aatattctat aattatattg
     5281 taactaatac ttattctaag gagtgatttt tttgaacgaa gatgttgtaa ctaatgaaac
     5341 tattgacaaa gattatgcta ttgaagaagt tcgtatggca tgcaaacatt ttggtgattt
     5401 atatttttat ttttccaaag tgttatttga agagtttggt gaagataaaa ctagcgaaat
     5461 attaagaaaa gttttatttg aaaggtctga agaaagagct attgctatga gagagagagc
     5521 tcatgaaaat ggagatgaat taattgcaga caatattatt agcacaactg atgttccatt
     5581 tctaggatgg gttcctgaat ttgaagagtt acattgcccc tatggagctt cctggctttt
     5641 aagatttgag gaaaatcctt ggtttaaaaa atttgcttct ctttactgtg atgttacaga
     5701 tactactgta gctgaagtat ttacaggaga tacttcccat aaaataacaa aaaacattct
     5761 atggggagat aaaagttgtg aaagaatata tttccatgat gacaaggtat ctgaaggtaa
     5821 atatacttat ggaaataaaa actgtaaata aataatttaa attttctata ttcatatact
     5881 atattttgta ctttttcata taaatattat aactagtctt ttattttact tttgtttaac
     5941 ctaaagataa tttactttta atctatatga attatcatat aaataatcag aaagttatct
     6001 ttataaatta tagttttaga tttataattt ataaaatatt tatgttcttt gtaaaatttt
     6061 ttagaagctt ctaatgttat gctataaaac tatcttgaga agggctgatt gaatgtttaa
     6121 taaaatatat gaaaagaaag aatttatagt ttttcaagtt aaaaaaggtt atgtagttta
     6181 taatactaga aagagttttg aagaaggtca tacacattta aagcattttg aagcagcaaa
     6241 gacagctatt gatttagcaa taaataaaaa gattcctaga agcaaagatg gatattatct
     6301 gactagttta attcgcataa gtgatgatgg ttattacatt gataagttaa gcgaattgtt
     6361 atatgttaga gaacaaaaag gtaaaaaaga aaagtactgt aattctggtt atcagatgta
     6421 acaagactag ttgtgaatta taagtaccaa tcataagtac ctttagtctt aaaatcaaag
     6481 agtatcagta aatctataaa aaaacaactt actgatactc tttttaatta tatgattaat
     6541 atttgaattt gatacaaaat tttatacaaa agtttatgtt atctattctg attctttgtc
     6601 tgcaaattgg ctattataaa gcttctcata aaatccaccc ttttccatta attcatcatg
     6661 attcccctgc tctataatac ttccattatt cataactaaa atcaagtcag cattgcgaat
     6721 tgtagaaagt ctatgtgcta taacaaaact agttctacca ttcataagat ttctcattgc
     6781 tttttgaagc ataagttcca gcctagtatc aacagaactc gtagcttcat caagtatcaa
     6841 tattgaaggg tctgatataa aagctctagc tatggttaaa agctgttttt cccctagaga
     6901 tatattagat gcttcttcat ttaaaaacat attgtatcca tctggaagag tggttataaa
     6961 gtgatgaaca tttgctactt ttgctgcttg tataatttct tctttagtag ccccaaatct
     7021 accatattct atattttcaa aaatggttcc attaaataac catgtatctt gaagcaccat
     7081 accaaacatt gaacgcaaat ctttacgttt catatctctt atatctactc catcaatttt
     7141 aatagcacct ttctttacat cataaaacct catcaataag tttattaatg ttgttttacc
     7201 tgctccagta ggtccaacaa tagctaccat ctgaccactt tttacttctg catttaaatc
     7261 ttctattaaa gttgaatttt ctccatatcc aaaattaaca tgctcaaatg taacatttcc
     7321 ttttacattt tctatcttaa ctggattttc aacatcaggt atttcctctt cttcgtctaa
     7381 aatttcaaat acacgttcta tagctgcaaa agaagattgt attactgtag ataactgagt
     7441 catttgtgaa agtggctgat ttatctgcca tatataacgc acaaatgctt gtaaatttcc
     7501 tactgttatt cctccactta ctgcatatat agaaccgaca actgccacag ctgcaattcc
     7561 aaagtaagtt actaatgaaa ccataggatt catcatactt gatataaatt gtgccttaaa
     7621 tccattttca caaagttctt gatttacttt tttaaattct tttatagaat cttcttcttt
     7681 accatatagt tttatttcat taaatccagt atacatttct tgtactttcc catttaattt
     7741 tccaagagca ttttgttgca atgaaaatct actttgagat ttttttacta caaatttaga
     7801 tacaaaataa ctaattggaa ttataaaaag agctataaga gccatatata tattaattgt
     7861 aaacatcatt gaaagtgcca aaattatgac taagattgag tttacaattt gcataaaact
     7921 ctgctgcaat gcatttgata ttgtatctac atcatttgat atacgactta atacatctcc
     7981 aaaactatta ctatcaaaat aactaatagg caatcttctt attttatttt cgacttcatt
     8041 tcttaaatct ctcattgtgt tttgaatagc attcgtaagt agaaaacttg aagtatacgt
     8101 aaaaattgca ttggcaaaat atatacatgc taaaatcacc aatatttttg ttacataatc
     8161 aaaatttacg gaagcccctg gtactccttt tgcgatacta attacatctt tagtcaattg
     8221 tgttattatc aatccttcag ttttaggtgc aagtgcattc atagtagctg ctgaaattat
     8281 acatataata gctataataa aggaaaattt atatttcttt acaaatggaa gaagtctacc
     8341 aattgttttc ttaaaacctt tagacttttt cattattcta attcctcctt ttttaactga
     8401 gatgttgcaa tttcatagta tatttcacag ttttttaata attctttatg agttcccatt
     8461 cctacaactt gtccttcatt gagtactata atcttatcag catcaataat agaactaatt
     8521 ctctgggcaa caatcaatac aattgcatct tttgtttcct tcttcaattt agcacgaagt
     8581 attgcatcag ttttaaagtc aagtgcagaa aaactatcat caaaaatgta tatttcaggt
     8641 cttcttataa tagcccttgc aatagaaagt ctttgttttt gacctcctga tacatttgct
     8701 ccaccttcac taatcaattc atcaaacttt ctaggttttt ttgatataaa ctcatatgct
     8761 tgtgcaactt ttgcggaata ttctaattct gactctcctg ccttatgttt cccaaatcta
     8821 atattatttg ctatgctacc agtaaaaagc agtgattttt gaggtatgaa tcctatcttt
     8881 tttcttaaag cttttaaatc atattctctt atatctactc catttatctt aatgctacct
     8941 tctgttacat cataaaatct aggtatcaag ttaattagag tacttttccc actaccagta
     9001 cttcctataa aagcgactgt ttcacctgtt tttgcagtaa atgaaatatc cttaagtaca
     9061 gaagcttctc catctggata ggtaaaagtt acattatcaa attctatgat acccttattt
     9121 tcagtatcct taattccatt ttcaggattt tttatcaatg gttcttcatt taatagttct
     9181 tttattctat ttgcagatac ctctgctctt ggatacataa taaataccat tgaaaaaagc
     9241 ataattgaaa acatggcatg gaaaagatat tccaaaaatg caaccaactg tccaacttga
     9301 agctctccaa tatttatctt ctcacttgaa atccaaaata cagccaaaac agctatattt
     9361 aatagtaaaa agaatgctgg ttgagtaatt gacataagct taaatagttt cttagaaaca
     9421 ttagcataat atgtatttgt tttttcaaat cgctctgttt cataatcatc atttccaaaa
     9481 gctctaatta ctcttattcc agttaaattt tctctcgata taaggtttaa tttatctagt
     9541 cctttttgtt gttttgaaga tattgcctga gatacttttg ctattattgc tactccaata
     9601 attataaaag gtactgatat agccaatact atagaaagtt ccacacttgt tctaattgtc
     9661 attattatac ttataatgaa cataactgga gttaataagg cagtccttag tagtatatta
     9721 acaaattgtt gaatttgaaa tgcatcattc gttgttcttg taatcattga agaaattcca
     9781 aatttattgt attcagtatg tgaaaattct tgtgatttct caaatatgtc atttcttata
     9841 tctctggtta cacttgttga taccttagca gaacaataac ccaacaatat actacctaca
     9901 acccctataa tagaaataat aacaatcact attcccattt gttttatgta agctatattc
     9961 tgatttgcaa taccattgtc aattatttta gctattatag ttggaatccc caactcgact
    10021 aatgcaaaac taaatatagc cagtatgttt aatactatta ataacttata attctttaaa
    10081 taacttaaaa ttaatttcat atttttctcc tttcttaatt tttgagatag tgtaatcatg
    10141 taataatact aaatttataa atgcttaatt ttataaaaaa aggagaaaag agttaacgca
    10201 ctctcttctc cagcatattc tttttaatac accctccgac aagtcgctta tatgttaaca
    10261 tatttgacaa gtaatgtcaa ttcatatagc caaaagtctt ttaaatgctt catttcataa
    10321 cattgtaaca aattggcatg taatttattt ttgtttttta ctgtattcca attttacaag
    10381 ttctccaata ttactagctt tcttaaaatt caaattagtc ttatctatac cagacttaaa
    10441 tagtgaaatc cctctcccca atattattgg caatatatat gtataataat catcaattaa
    10501 gttatctttc ataaataatt ttactatatc acctccacca actatccaaa tatctttttt
    10561 atccttctct tttccaattt gctaaattaa ttttttaggt tctaaattaa tttataatgt
    10621 gattattcta tgtaaacatc actttttact cattcaataa taccctatta ataaatcctc
    10681 aaactaattt aaataaactt tgcataaaat taaaaatata ttatataaat aatttaaaac
    10741 tacactaact tattgaaaat tctaattatt acaaataaat attgctctct gttaacgcta
    10801 gttctataat ataattataa gctattaata ttataaagcc gatattacta atataataat
    10861 tataatactt atagttaatt tacttttgtt gaaaggtggt aaataacatg aaaatttcag
    10921 aagtcgctac tccaaatttt ttgctagatt tagaccagtt agaaaagaat atagataaaa
    10981 ttcaacaaat ttgtacaaat aataataaac aactttggcc tatgcttaag acacataaaa
    11041 gtacttacat agcgagactt caaaaaaatg caggtgctag tggttttcta gttggaacac
    11101 ttgatgaagg tgaagctctg ataaaagcag gttttgaaga tattatgttg gcttatcctt
    11161 acatgggaga agtaaatata aatagaatcg taactatggc atcaaaaata aatttaatct
    11221 gtgctacaga taatatagaa tgtgctaagg cttatagtga agctttttct aaatcaaaaa
    11281 tagagtgcaa gctacttatt attgtagatt gtggcttaca ccgttttggg gttgaaccag
    11341 agaaagtggt tgaacttgct actgaaattt ctaagctaga aaacatcaag attgttggta
    11401 taagttcgca tccaggtcaa gtgtatggtt gtagttctcc agaaggtgtt ccagatgttt
    11461 gtgttcaaga agattcttct atggagaaag catacaaagc tctaactgat aatggattta
    11521 atatagaaat tgtcgcttct ggtagtactc caacagttga atctgaagct gcttctaaaa
    11581 ctataactgc tgtaagacct ggaaactatg tatactatga tgctatacag ataggtttag
    11641 gttgtgctac tgaagatatg tgtgctctta ctgttgtgtg cactgtaatc tcaaaaaata
    11701 gcagtggata ctatttaatt gattgtggaa gtaagtgcct tggacttgat aaaggtgcac
    11761 atggaaatag tagtattgtt ggttatggta gggtatgtgg acatccagaa cttactattg
    11821 attcactttc agaagaagtt ggaaaaatta aagtaaatgg tgaaactgat ataaaaattg
    11881 gtgatagaat aagaattata cctaatcatt cttgttccac tgcaaacttg acaagtaatt
    11941 tattaggttt tagaggagat acaatagaaa aagtcataga agtagatatt cgtgaaaatt
    12001 caaaacaaat catcctttaa aaacacattt attctgatat taacatgaaa gtatattttt
    12061 cttaaaatta ttgctaataa ctaatgttaa tgttggataa ataaaaaaca taaattctag
    12121 aaatataaat ttaaaagaag aggctgtctc aaaatagaga taagcctctc tttcattgtt
    12181 gaaattatga aaatatactt gatttcatac aaaaagagta tctcatgaac taaaaagttc
    12241 actttgagat actctctttt tttaattaat atacacatta attactatct tagtttatta
    12301 ctatatatca attactactc attaatcctt gtatatatta attcttatat atcatttata
    12361 gttaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa aactcttact
    12421 atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat tctatactta
    12481 gtactggcat ttgctacaag cactattctg gcataatgac caactagagt acccattaac
    12541 atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg tgcaaataag
    12601 tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa aactacaaca
    12661 gattccccag gtagtccaaa aatacccatt actggtccaa aaatatttcc taaaatatca
    12721 actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat ggctggtaat
    12781 atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat ctcaacagca
    12841 gttttcttag gtttactatt tacaattaca tttccagtat tttgttgact catactacac
    12901 agcctcctta gccttattct ttttatttct tacacctatg attaatctaa ctagattagc
    12961 accaagtatt ttacagaata tctctataag gattattgga cctaatgcca ataatgctat
    13021 tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac cagcatattg
    13081 atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa ataattcttt
    13141 cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta ttcctgctat
    13201 tccaggtata ccaagcagag gtctaagcaa tggattaaat atctttgatg ccgctttcat
    13261 tcctcccata gattcaacta catctataag acctacagca aaacagacag tcggaatcaa
    13321 agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca taaagtctac
    13381 accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg ctttaaggaa
    13441 gccttggcta tctttaaata ctcctgaaaa taatactatc aatacaaata aagataaata
    13501 ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc cccctttata
    13561 cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc tcaaacatag
    13621 aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca ccaaatgaac
    13681 agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct ccaagtctct
    13741 tagcatttaa ttctccaaat ccattttctt ttgacacttc atttacaaat gtatgaaatc
    13801 tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta acttctgtag
    13861 aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta acattctcca
    13921 tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct ggaatagaat
    13981 tttccactct tccaccctta atcacactta cacttactgt agttccaaca tctaaattag
    14041 tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt cttccacctt
    14101 caaaatcatt acctgcgtgt gtttctacac cttttacatg tatattacat ccgattcttc
    14161 cttttctacc tacacacaaa ctattatcta ctaatccagt ttccatatta aaagcacaaa
    14221 gaccaccttt tgcttccctc aatataacat ctgcacctgt tgaatcctta tgtcctatct
    14281 cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt ttatttaaag
    14341 ctttaataac atataaggaa attattatac cacccttcat atctaaaaca cctgggccat
    14401 atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt tcaaagacag
    14461 tatccatgtg acctgaaaaa attattggct ttttatctat attagaacca agtgtaccta
    14521 tcaatgtact accattatct ccaacatcta ctaactcgca atctaaacct tctttttcaa
    14581 actcaaactt taatctctta gccaatttat ttacgctttc tttacaatgt gtatagcttt
    14641 ccatattaac aatctctttc cataatgaaa ctatttcttc tctattctca tcaacaaact
    14701 tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa taaaatgtat
    14761 ttctttccta aaacctaagt ttattttagg aaatcttaaa agactagtcc ttaattacaa
    14821 aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata ttttctgatt
    14881 tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta ttataaaatg
    14941 aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag gttttatgaa
    15001 atataaaatt ggttttttga caacatctta ttttatgtca gataagttta aacaagctct
    15061 taaagaatta aatgatactt gtgaaattac atttattgag ctcaaagggg agaatccttt
    15121 taataattta cctaatatat actatgagca agtagataat tttgatggat ttgtatgcag
    15181 tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag ttccattaaa
    15241 ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat taaatagcca
    15301 aaaagatatt gattttacaa aatcatttat ggatttttta agagaggaca acaattatta
    15361 tgatatatat tcattaatag actctaataa atgtccatat acaattaaag attttaatat
    15421 ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa tacatttaga
    15481 cttattgcga gataataaga ttaacctttc ttttactaga aattacatca ttagttgtga
    15541 acttaataag cttggatatg attgtattta catggttcca tctacagaat caatcctgaa
    15601 tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa ataaatctgc
    15661 aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga tatttaaaaa
    15721 tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa gacatggatt
    15781 ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta ctaaagaaat
    15841 gttaaacaat atgaccaata actatacaga tttttttatt tcagaagatt taaaagaaat
    15901 taaatatagc ttaaatatag gttggggtat tggaaataca aatattcatg cagagcaaaa
    15961 tgctcgtcaa gctaatggta aatctgcttc tttgaatgga aactgtactt ttatagtaaa
    16021 tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata atattgaaga
    16081 tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa atgtatctaa
    16141 aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa tattatcaga
    16201 ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat ataaagcagg
    16261 aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa aaaaaatata
    16321 taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt aatatttaat
    16381 tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt taaattttat
    16441 tagaatatac aactttttac tttaaaatac ctaaaccttt tataagtgaa ataaaccata
    16501 atatatcgta acttattaga aaaacaaaat aatttcatat acaaataata aaatagccac
    16561 tacaatccat aatttgcagt ggctactttt tacactatca cttattactt atttttaaaa
    16621 attaattgta ttttttaaat aatttataca cacctattat cagtaaagca ataaatacaa
    16681 gtatatctat aaaaataatt attgtaacat gtctggcaat attgtaaata aaaagtatag
    16741 agaatatttc tatagacaac gctacaaaac acataaaatt cgttaaataa tctgcaatag
    16801 tatcactata aaaagcatgt atttgtaacc atctccatat aaatggacgt aaaaggattc
    16861 tgaatacaac acaaatgaca gggtaggcat aaataaattt tttatctaca taattagcta
    16921 caccattatt ccattggata ggaatttgag gtggcaactt tggaaatact gttattgcaa
    16981 tttaaacact acctctctat gctattaatt tataactata atatagtctt aaatattttc
    17041 actctataaa attgtatatt tagtatatct taaaatcaaa ctaattatct tttataagtt
    17101 ttcatacaat aatatctcct ttcaataata tatctttttt tatatttaaa actatcttat
    17161 tttctataaa ataatgtata aacaaggttt acagtcaagt atttttatta tttttttgat
    17221 ttcataaaaa aagcatagca aattatttgc tatacttttt tactaaatta tttattctat
    17281 atcttatatg aacttattat ttaactacta cacaatacta gctttaatca tcatattttt
    17341 tgcaaacata ctggcttcgt atatttcttg ttcatctgga tgactttgag attcctcatg
    17401 gttatttaat tgttggcgaa tatgttcgtt gtcaggagaa agttttaaca tctctttata
    17461 ttttgctttc aatccttctg ctatcttacc ttgacatatg aatgaaccta gtatttcatt
    17521 atcttttggg ataattgatt ctatcttttc tattatacta gctccataac ttcctttttt
    17581 acttgctccc attgttccaa atagtgctat ctttttattg tgaatatttt caagcacttg
    17641 ttttgaattt ttatcacaaa ttcccttgtc cacccaatag cctaagaata ctatatcata
    17701 attttcatag tcaataccat aatcaactaa ttcatttaca tctttaatgt ctttttcttc
    17761 tttaatttca tcatatatag tataagcaat ctttttagta tttccagtta aacttgaata
    17821 taaaactaaa cttttcatga tgtctcctcc tctttatcta aatacttatc taaatcacca
    17881 aaaatatatt aaccttaaca aattatttat ttttataagg atattctaat ctagtataca
    17941 tccaaactga gagcgtgttt gtaaagcatc tgcacacttc ttattacaat aacagcactt
    18001 tttaatctca tctactcttc catcttttac tttctgtaac caattactat ctgctagtaa
    18061 ttgtcttgac atagaaacca tatctactct attatttgaa ataatacttt ctataaaatc
    18121 tgggctagac aattttccaa ccccacatac tggaagattt gtgtgtttct ttacctcatc
    18181 agctaaatat aaaaaacagc cttcatcttt aaagtaagga tgattattgg ttggtatagt
    18241 atcttctaat tttgaatgat ttgcaagtgt tacatgaaaa ctgtttacac ctagggattc
    18301 aaatttttta acaaaatact caacttccga aagtaaaact cctgcatttc cataatgagg
    18361 attttcttgt cttattgcaa atttatagtc tattggaatg tccttaactt catctcggat
    18421 actactaatt atttcgcttg caaatctact tctattatca cacgttccac catattcatc
    18481 atttctatta ttaaatatac ttgatgaaaa acttcctaca agcctatcac catgaatttg
    18541 aatcatatca aaacctgctt tttttgcatt caatgctgtt actttaaata aatttataat
    18601 atttttaatt tcttctttgg gcatatttgt tatgtaatct tttactccgt cattcattat
    18661 tttttttatc tcatcatgtg aagtaattcc catattcata gtatctttaa ttaaattaac
    18721 atcataatct gaacaaaata gttgtgctga tacctttgca ccagaattgt gtatttcatc
    18781 tacaattttc ttgtatttaa tgactccatc ttcatccgat aggcttatag ctaccttatg
    18841 aaaactaggt ctaatagata catctcctag agtaatcaga gctactcctg acttagcaat
    18901 atcagaaaac tttttaattt tttcttccaa tttaagaccc atgcttgtag gtgcaaaaga
    18961 tactctattt tttaattcca aatttccaat tttaattggt tttaataaat tacaatacat
    19021 cctattcttc ctccacagct gtttttatat cctttaccac atgtaaattc tttaccagtt
    19081 ctttatattc tgtatccgag aactcttttt ttatgtgatt atcccacttt gaaaatatgt
    19141 tttttatatc ccctaatact tcctcaccct tatctgttac tctcaactga aaagctctac
    19201 tatcattagg gttttggatt ttttctatgt atccatcttg ttctaaccta gatatagttc
    19261 ttgttgtatg agctttatca acttcaaatt ctgtagaaac atcattcaaa gaacaattcc
    19321 tatttttatt aatataaatt aaatagaaat atagaccatt cgtaagttta tatggtttta
    19381 aacatttttc acaataaagt ttaaagtttt tttgcaagtt acaaataata tatgacaaac
    19441 tttcatactg taaattttta aaatccatct taaatttccc ttctataatt ataatatgtt
    19501 tgactatatc aactatatta tattaaacaa ttaatatagt caagaatatt ttattctcta
    19561 aatctatgtt ttattaattc tttaaaaaac tattatataa aataatcatg ataaaattag
    19621 aaattggaat ttttaatatg cactgtacat aaatatattt tatataaaag tttatatttt
    19681 caaacaagta atttttcgaa aattgtctaa attcagtaaa aatatggtaa tatatacata
    19741 agcaatataa atctataaaa aggagtgtta catatgatat ctaataaaat gcaaacttta
    19801 gttgcaaata gttctgttat aagagctatg tttgaagaag gaaagaaact ttctgatatt
    19861 tatggtgagg aaaatgtttt tgattttagt ataggaaatc caagtgttga acctccagag
    19921 actattaaag cagttataaa tgatatttta aatgaagaat ctccaaattt agttcatgga
    19981 tatatgaaca actcaggata tgaggatgta agagatgcta ttgctgaaca cataaataaa
    20041 aaagatggat taaatcttac aagagaaaat ttaattatga cttgtggtgc tgctggtggt
    20101 ctaaatatta ttttaaaaac tcttttaaat cctggtgatg aagttattgc ttttgcaccg
    20161 tattttggtg agtataaaaa ttatacagaa aactatgatg gaaaattaat agaagttcct
    20221 acaaatatag aaacttttga accagattta gatgctctaa aaaatgctat tactccaaaa
    20281 acaagagctt taataataaa cactccaaat aatcctactg gtgtcatata ttctgaagaa
    20341 ttattaaaaa atcttggaga attattggat tcaaaacaaa aagaatttaa tacaagcata
    20401 tacctaattt ctgatgagcc atatagagaa ataatttatg atggtgctaa agttccttgt
    20461 gtactaaaat actatagaaa ttcatttata ggatactctt atagtaaatc attatctcta
    20521 cctggtgaac gtataggata tattgttgct aatgggcaaa tggatgattt tgatgatgtt
    20581 atgtcttcat taaatgttgc aaatagaata cttggatttg taaatgctcc ttctcttttt
    20641 caaagggtta ttgcaagaag tttagatgca gaagttgatg taaatattta taaaaagaat
    20701 tttgaccttc tatataatag tttaatcgat atgggatatt cttgtgttaa acctaatggt
    20761 acattctatt tgttcccaaa agctccaatt gaagatgata aaaaattctg caatgatgca
    20821 aaacaattta atttattatt agtaccaggt tcttcttttg gttgtccagg tcattttaga
    20881 gtatcatatt gtgtatctta tgataaagtt aaaagttctt taccagcatt tgaaaaatta
    20941 gccaaattat ataatttaaa ataactaaat caaagtaatt caaataatta actaccttag
    21001 tatagaatac ttacattcta tattaaggta gtttataatt ttatgtacta tattttatac
    21061 ataccatatt attccaatat gcttgaatat tcttatctat agtactaata taaattacaa
    21121 ggaggtgttt gacttgaaaa aaggttatgt ttatataata ctgactacct tattttttag
    21181 ttcaatggaa attagtctaa aaactgtcac taatgacttt aatcccttac aaataacttt
    21241 atcaagattt tttgttggcg gtttagtctt actacctttt gctgtaaaaa gattaagagc
    21301 tcttagtctt tctattacaa aatcagactt aaaatacttt gcatttttag gttttatgtg
    21361 tgtagtagta agtatgtcat tgtatcaact tgctgttcta aacacaaagg catctgtagt
    21421 tgctgttttg ttcagttgta atccagtctt tgtaatgctt ttagcttact taattttgaa
    21481 ggaaaaaata tataggcaca atgtaatttc acttatatta gaagttcttg gaattatagt
    21541 tataataaat ccattgcata caaaactaac tcttagtgga atattcttaa cattatcttc
    21601 tgctattatt ttttcaatgt atactgtatt tggtaaaaga aaaaccttaa aatttggtgg
    21661 aatagttgta acctgcttta gctttatatt tggtagtttg gaaatgttga tattggtttt
    21721 actcactaaa attaatttta tcgcagatat attaaataaa aataagcttc atgtatttgc
    21781 aaatatccct atttttagtg gatatacctt acacaatatg cccattatga tttatgtatt
    21841 cgtatttgtt acaggtgttg gttatgcgct ttactttatg gcaatggaag ctacatctac
    21901 tagtcttaca tctttagtat tcttctttaa accagtaata tctccaatac tagcattatt
    21961 aatacttaaa gaaattatcc ctataaacat ggttatagga attttattga tagtaattgg
    22021 ttcaataatt tctattatac ctacgataat tactcaaatg cataataaac acgttgaagc
    22081 cttgtataag gagtagtaat tgataatcta tcatagttat acataagaca aaatttatta
    22141 gatttaaacg caatataaaa gagatgctat tataatagca tctctttatt tatatttatt
    22201 ttaaatttat taaaactcta tttagctaaa ttttgcaaaa agctataagt ttgtggacaa
    22261 gctgatttta acttactatt tgtatcttga tttaaatagt aatatgcaaa tgattctgca
    22321 aaaaattctt ctggatatac acctaagtag tttacattac ctaaagaacg accttcttta
    22381 gcaaatattt gtttgaattg tgctgattta gatatatcat ttaatactat atggtctatt
    22441 gcatgtgctg tttcatgtaa ttctaaattt attgcatcat gccctttacc tttgttacta
    22501 aatcctattc taagagctac tgtacttcca cctaaacctg gaacatcatc ccaagttttt
    22561 ccagtacctt cccatccctt aggaactaca ccttttaaat aagcatattc tttttcatct
    22621 gttaactttc cacttaataa ttttattttt aagttatttt gttttaatgc atttaaatac
    22681 tttccatcta tattagctaa tctatttacc atagcattag cttcgttttt atcgtaattt
    22741 ccagttggaa aaactactat ttgactaagt gtatctttat tttgttgtat agtagtacta
    22801 tctgcatgtg cacttactgg aactgaactt attaagaata ttgatattat agctattaat
    22861 aattttttac ttggtctcat attattccct cctttgagtt aggccgggag taataaattt
    22921 caaaataaaa aagataccca agagtatctt tgtatataca aaaagaaatt tttactcttt
    22981 tcggcaactg gctggcatgg tttactaaac tttagcccct atagctttgc gtgactaggt
    23041 ttccctagtt ttgcctcatt taattttata tctatattat agcttatatc tttttaattg
    23101 tctatatccc tttggaactt ttttctaaaa agttattatt tttttacata aatcttcaaa
    23161 aaaaaatatc ttctaacaat attttcatta ttaaatattg ttttagaaga tattttttaa
    23221 tataacttat atttttataa tgttagggtc taatttgtat ttttatttct tcttaatacg
    23281 ataagtccta caatagctcc tataaccaaa agtatttctc caataattgt agtactatcc
    23341 cctgtttttg gtggtactgg tggatttacc aaagtatcat ctttaacttt attatttttt
    23401 tcttctgaca ctgatggctt tactacttct ggtattggaa taatctgttt aacaggagtt
    23461 tctatcatct cttctgatgg tggtactggc ggattcaata cctctggtat tggcggattt
    23521 attatatctt ctgatggtgg tactggtgga tttataacta tagtattctc tgtatcagat
    23581 gaacttgatg gtttccttgg tttatctgtg cttggtggta ctggtggatt taccattggt
    23641 tcatctgtat ctggaggtat tggtggattt actggtggat tttcttgttt tagattttct
    23701 atatttccaa tatctactat cttaccaata tcttcattac caatttcaac cttaacttta
    23761 tccttcagtt tttcatatcc acgtggagcc attatctcca ctaaataata tgttcctggt
    23821 ttttttacac tagattgaat tataccatct tcatcactaa ctaaatcctt aactactata
    23881 tttccaaatg catcttctaa attgaactta gcacctgaaa gtacattttt atctaaagca
    23941 tccacttttt tagcttcaaa agcataacct tgcttagtag tctctactcc tggaggactt
    24001 gaaccattaa tctgagccac atttcgtata gtctccccaa cttttacatt tgtgaagttt
    24061 gcatcaaatg taacccaact ttgcacaggt tccccttctt ttatctgttt aaattcgtat
    24121 ttatttacta atcttattgt attttttcca tctccagctt cttttttaag ttcaagatta
    24181 tcagaatatt ttatattaga tatttctaga actgagttta cttcatctaa aagatttact
    24241 tgtcctgcat cataaactcc ataagttgac atattgattt tgtatttaac aatctggtta
    24301 tcactactgt ttaaaacttt tttatcaact tctttttttg cgcttattaa tccagaattt
    24361 ttcttaaagt ttatctcatc atttttatca atttcactat tatttgcatc aagaacagct
    24421 ctatttatat agctatcttc aatactaatt acctttaatg tataagctat aaaaaacttt
    24481 gattccactt cttctttact gagattaatt gtaaatccag ttataacttt atctacttgc
    24541 ccactttcat ttcttttttc ttctacttta tatgaaatat tattggcaat tttttcttta
    24601 ctccaaggag tccagctccc attcattgga atttctacaa ctctaaaatc atgcccagct
    24661 tctcctgaag tagttaatct catgccttct ggtatattat ctgttataac aacattactt
    24721 attggtgatt tatcatcatt tattgaaatc tgatagttta ttacttgacc aacttgagta
    24781 atatttgttg ctcctgaatg tcctttagta agaagataat catctcctct atcaaatata
    24841 cttacagtag catcatcttc ttgtatcttc ttaccatcag attcaatcct agctacattc
    24901 ttatatttat taccatgttt tgcatttttg tcactgattc tagccttata cttaacagta
    24961 tacgatttat ctatatttcc aaaattaact gaaatagaat ttttactctg ttcaatatta
    25021 aaatcactaa cttcttttcc atcacttgtt actacactta cagaaccatt tatcaatgtc
    25081 aatccttcag gaagctcatc atacaaaata atatctttta actcttcctg cttttcattt
    25141 acttgtatac tgtaatttat atctttattt ttaactggca taaatacagt agttgatttt
    25201 tttccttcaa caagcttttt aagtgttcct ttagcatctg gaacatccgt tttatctcca
    25261 ggttttattt ctacatctat atctccactt ccactttcta tttttccagt agtttcaata
    25321 tgtatttttg cattagtatc ttcatcaacc tttttaacat atgcttccaa acctatttta
    25381 ccttgcatta cactttggcc attaggtaat tctctaaatg taagtgtata ctctccatta
    25441 ctttctgttt tatcaaagca ttcacttgta taatctaaat taactttatt gaatacatct
    25501 ggcaccttaa aagttattga atctcctgct tgtattcctt ttgatgttgg agtataagtt
    25561 aaattaaatg atattttttt cattgactcc gtttctattg tattgttatt aatttttaca
    25621 acaaatccat agtcttcatt ttcttcatta agttcttctt ctaaaacttt caataaattt
    25681 tcatcttcta atagattatc ttcctctgga gttttttctg tatttttatt gtctagtggt
    25741 ttttcatctt ctaaagcttg ttctgacttt ttattatctg ttgacttatc ttcttctggt
    25801 atctgctctg gctttttatt atctattggc ttttctcctt ctggagtttg ctctggcttt
    25861 ttattatctg ttgacttatc ttcttctggg gtctgttctg gttttttatt atctgttggt
    25921 ttttcttcct ctggggtctg ttctggtttt ttattatctg ttgccttatc tttctctgga
    25981 gtctgttctg gctttttatt gtcactttcc ttctcctctc cagttgatga attagttgaa
    26041 ccactttgaa tctgactatt ctctcctaat tctgaagcat aagatacagt tggatatatt
    26101 gtagacacca ccatacttaa aatcataatt aaactaatta ataatgcctt tctatttcct
    26161 ttcttcatgt aaatctcacc cctttaggtt acctataatt tataatctaa tcttccatta
    26221 atatcgtata taaagtaatt tttactaaaa aattatacaa aaaaagctat gaaaataaat
    26281 aaatttatat tcatagccgg ttgcaccact gactcagtat atccataagg tgcccaataa
    26341 tagaatatac ttcaacatct ctataatatt tcataaaaac aattaaaatt gctttattta
    26401 tattaaaaat acaattatat acttctatta tattatgcct ctatactatt gtctatcctt
    26461 tttacatttt ttaccttatg atgagattaa ttaataatta aataaaatcc ttatctacaa
    26521 caattacaca attatattca ggatttatct ccttaactaa ttttgttata tcttctttta
    26581 agtcatcttc tgacatgttt ttggaaaatt cggaaggatt aactactata tcaaatatta
    26641 aattcttctt gcttcctttt ccaactattc taaaatcatg catagatttt acaagactat
    26701 ttttcttaag tatttcttca agttcttttt tggctgacat aacttcttta tcttcaagac
    26761 atattgggtc catgtgaatt actagatata tatctaattt ttcagatatt tctctctctg
    26821 cagtatctat aatttcatgt atttccatta tatctatgtt ggatggaatt tctgcatgta
    26881 tagatgcaat acatctacca actccatagt tatgaataat taaatcatgt gtacctatta
    26941 tatatttata agataacaat agctctataa tagaatttac catttcttca tccggtgctt
    27001 cccctaaaag aggatttata gtatctttta caagagagaa accagcataa agtatcacta
    27061 atgatactac gataccaaca tatccatcta tgggaaaatt agtaaatcta gctacaataa
    27121 atgatataac tacacaactt gaagtaaata catctcctaa agcatcaagt gaaactgctt
    27181 ttaaggctga agaatcaatt ttatttccca taaatttatt aaaacgactc aaccagattt
    27241 taatcattat agatgcaatt aaaagtataa gtggtattac ttcaaatttt acaggaattg
    27301 gatttactat cctttctata gatgacttta caaattgaag tccaaccaac atcaccataa
    27361 atgctactat taatgctgat aaatactcta ttcttccatg accaaaaggg tgttctttat
    27421 cagctggctt acttgctaat ttaaaaccta tcatagttat tgctgaagat gccatatcag
    27481 aaagattatt aaatgcatca gccattatag ctatactaga tgtgagcatt cctatgaata
    27541 cctttatcac aaataaaagt aaattggata atatacctac tacaccagct acataaccat
    27601 acttatttct aacatctgta ttttgaacgt tttcgctatc tctgataaat gttttgacta
    27661 ggattttgga aaacatgaaa cttacctccc tgccctttat tttattaaca aattttataa
    27721 tctatatctt tatttatgat aacataattt atatataaaa ttcttaaaaa tcactctact
    27781 tcagtaaaat attattctat atatctattt agatacgaat tttatatttc gtaaagtttt
    27841 tatggcttag tgtctgttta atagattcta tataagtttt tacaaagatt atttattatg
    27901 ataatatcat ttttcccaag tattacttag aatatgattt ttttgttttg tctgtacata
    27961 ttttaaattt atactatatt tatctatatt ttataacctt atgatacact ataactataa
    28021 gtttttaaat ggaaaggaat gaaataaatc atgtttttta tcagttatat ttacacaaaa
    28081 tcacctatta aatttgatac tcctttgcaa aaagaggcat acaaaatatt acaaaaatta
    28141 gatattgact ttgaatgtgt tgacacagat gaagcaatta caatggaaga ttgtgtttag
    28201 attaataaaa aattaaatat gaaaatggta atataaaaca acattaaaca aacaaaaacc
    28261 gcaaagttac aatatatagt actttgcggt ttcataagaa ttaattttta ctattaaagt
    28321 tcatagttct actatttttc attgttctaa gaattagctt tacaaattca tttgacaata
    28381 ggatagtgga tgcaaatagt attatcttta tccacatatc aaagtccaaa gaaacagaat
    28441 taaagaaact ctggaacact tgtgtaaata ttatttgaat tatccctgta actactatta
    28501 tttgcaatgc cagtttattt ttaaagaaat ttggtattgt actatttaaa ccaaactctc
    28561 tacaatttaa tgcattaaaa agggcactga atgcaaataa agaaaatata acagtacctt
    28621 gttcttctga tgtagctccc aatatattaa aagctgattg agtaaataca attgtaatta
    28681 taagaattgc atttataata atatttacaa acatagacct agctattatt ccagaatgtc
    28741 tatttattgg ttttcttttc aatacataat ctctaacagg ttctaatcct aatgctaggg
    28801 ctggaggacc atccattatt atatttaccc ataataattg tattgtagta aatggcatat
    28861 ccttaccagt aagttgagat attatagcaa ttataaatgc tactatattt acagtaagct
    28921 gaaactgtat aaatctttga aagttctcat atatacctct tccccatttt attccctcta
    28981 ctatagtact aaagctatca tcagtcaata ttatatctgc tgcattttta gatacttctg
    29041 ttcctgaaat acccattgca attccaacat ctgcttgact aagtgctggg gcatcattta
    29101 taccatcacc tgttacagct acaacttcgc cacttttttg aagcgctgaa actattctca
    29161 tttttgtatc tggtttactt cttgcaacaa tagatattcc tttgatttct tctctcaatt
    29221 ctttatctgt caatacatct atatatgttg cttcaactgc ctttttccca tcatttaata
    29281 gtcctaattc atttccaata gctgttgctg tatttatatt atctcctgta agcattttta
    29341 catctactcc tgcattaaat gctttatcaa tggaatcttt gaccccttct ctcaatgggt
    29401 ccactattcc tacaaatcca ctaaaaacta aattatcttc cttcatataa cttcttgaat
    29461 ttcctattaa attcaattca ccttctgaag tcattgcaac ttctgtttta gaattactca
    29521 tttttttata agcaaatcct aatgttctca tagatttaat ttgtaacttc ttaatctcat
    29581 ctaaaatact tttttctact tttggagtta ttggaacaat attttttcct tgttgtacat
    29641 aagaacattt ttttaataat acttctggag caccttttga aagtaatata tctgaatcct
    29701 cttgacgaat taatgttgac atcttctttt cttctgaact aaatggtatt tgtgatatca
    29761 aatatgtttg ttttctcatt tcattatagt tcttatcatt atgatataaa agtaatgcac
    29821 actctgttgc gcttcctata tatttaaatg aattatcttc tttttctata tctgcagttg
    29881 aatttactat acagttttgt tcaaaatagc tattactttg atactctcct ccacttatat
    29941 atttaccatc aacatatgct acctctacca tcattttgtt ttgtgttaaa gtccctgttt
    30001 tgtcggaaca tattacagaa acagaaccta tagtttcaca ggcttctttc tttgttacta
    30061 atgcatttat ctttgccatc ttttgcattg ttatagctaa ggtaatattt atcatagttg
    30121 gcaaaccttc tggaacagtt gcaactatta atgcaataca aaccatatat gcatttttag
    30181 caggttctaa tgattgtaag aaagataata cactgcttgt atctaaaatt aacatacctt
    30241 ttctctgcat ctgtataatc ataaacatac acaataaagt agctattatg cctgatattc
    30301 ccgctatttt agcacccaac ttccctaatt taatttgaag tggagtttgc tggtcttcat
    30361 ttgataaatt ctgtgctatc ttacccatct ctgtcttatc accagtatga gtgacaacca
    30421 ttatgcccct accatatgct acaagagtcc ctccaaatac catattgact tgctttgcag
    30481 gtattgcatc ctgttcgatt atttctgttt tagaataaac tacttccata tctaatacag
    30541 catcagcatt cttagctaca tcatcagatt ctcctgttag catatcttct ctaagtttca
    30601 aatttataga ctgtataagt cttccatctg ctggtatcat atccccagtt tctatatata
    30661 caatatctcc tggtactaaa tcattttttg atattttaat tatcttccca tttctaagca
    30721 cctttacttc tatattttct gttaattttg acagggcatg agctgctttt ttagattttc
    30781 cttcagttat tatgcctata gaaattccaa taaaaatagc ccctaaaata cctaatgcgt
    30841 catgtaattc tcccacaaaa gaacttatta ctgctgctcc tatgagaatt aatatcatag
    30901 gttctgtcaa actttctccc aactcgtccc aaaatgttct accttctttt atagtaaatt
    30961 catttaatcc atatctaagc ttcctttctt ctacctcatt atcatcaagt ccaatttctg
    31021 gatttgtttt taagtatttt aatacttctt tggtaggttt attataatac ctcataactt
    31081 tatccccctt gttaattatc tataaggaat tatatgctta tgatagcttg tatattactt
    31141 ttcaaaaaaa ataaggtgtt gattaatcaa caccttatag tggttataca ttattaaatt
    31201 ttatgttaca ggctttataa attgtgagct atacaaatca aaatatttaa tcttcaaaat
    31261 acccttctgg atataatgga aacttagata taagttcttg tgcctctgct ttacattcat
    31321 ctaatacttc tttattatca atattttctg ctaccttatt cattatccct gctatctgta
    31381 taacttcttt ttctttcaga cctctttgag atattgatgt aagacctata cgaacaccac
    31441 tagttacaaa tggtgattgt gggtcgtcag gtataacatt tttattaact gttattccta
    31501 catattcaag agctttttca aattgttttc ctgtaagatt cttacttctt aagtcaacca
    31561 ttactatatg attatcagta cctccactta caatactaaa accatgtgaa atcagttcag
    31621 aagctagagt ctgagcattt actacaactt gctccattat ctttttaaat ttatctgtac
    31681 ttaaatataa tagtgaaaag gcttttgctg caactgtatt aagatgtata gaacctaatg
    31741 ttcctggaaa aacaccttta tcgagtttct ttgcatgttc tgctttacaa agtacaatac
    31801 cacttcttgg tccacagaaa gtctttgtcg tagatgaaga aacaaaatct gcataaggta
    31861 caggactagg tattactttc gctgcgacta atcctgcaac atgtgccata tctaccataa
    31921 agtaagctcc tacttctttt gcaacctttg atattctttc ataatctatt aatcttggat
    31981 atgaacttgc ccctgcaatt ataagttttg gtctacattc ttttgccttt gcttcaagag
    32041 catcataatc tatacgtcca gtatttgggt ctaccccata aaattcatat ttataaatgt
    32101 catgtaaaaa attaactgcg ctaccatgtg tcaagtgtcc accttggtca agacgcatac
    32161 ttaaaacagt gtcatttggt tttaatattg atgaatatac acaatagtta gctgtagaac
    32221 ctgaatacgg ttgtacgttt acatgttctg ctccaaatac ttcttttgca cgttttattg
    32281 ctagggtttc aagtttatct gcttcttcag aacctgcttg aaaacgagcc cctggatagc
    32341 cttcttctgt tttatttgta aatacacatc cacttaattc aagtacttct gttggtgctg
    32401 tactttcaga agctatcatc tcaatattgt gttcttgtct aaccaactca tcagctacaa
    32461 tcttgtataa ctctgggtct gaaatttttg ttgtttttaa cataataatc ctcctcttaa
    32521 ttaaagaata tattttatta gtttaatctt cattgataaa tttattataa cggtctataa
    32581 aacgtaagat attcctccac caggtcctaa tggtatgcca atacccatga atagtaacaa
    32641 tattattatt tctattaatg taaataccac tgagtatgga atcatattcg atatgacagt
    32701 acctatacct atatctttat catatcttct ggcaaaggcc aatataactg gaaaatacgg
    32761 aaaaagtgga cttattggat ttgttgaagc atctccaata cgataagcta tttgagttaa
    32821 tgctggatta aagtctagta gcataaacat gggaacaaat ataggtgcta aaatcgccca
    32881 ttttgcagaa gcacttccta caaataaatt gataaatcca gatagcaata taaatcctat
    32941 tatcaatcca tagctaggta ttcccgactc tgctagtaat tttgctcctg ttattgataa
    33001 aattgttcct aagttactaa gattaaataa attaataaac tgggatgcta taaatgctag
    33061 gactatatat ccacccatat cactcatact actacccatc atagatacta aatctttgtc
    33121 actttttatc ttcttactta cttttccata cactaatcct ggagttaaaa atataatagt
    33181 gattatagga actatacctt tcattaaagg tgcattacta gatagtatag aaccagtctc
    33241 tggatctttc ataaatgcat tgtcacctat acataaggca actatcacta ctactacaac
    33301 taacaatgat aataaagcgt attttatacc cttattttca agttcactca attcctgtat
    33361 atccttatca tttgttgaat cattatattt tgtaaaacga ggagctataa atttttcagt
    33421 tacaaatgct cctgcaaaag ttaaaactat cgctgatata aaagcaaaat aaagattcat
    33481 tgctggattg ccttggtaag atgggtccat catttgagct gctggtattg taaatccagc
    33541 taacaataca tctgtagtac taagcataac atttgctgca aatccccctg caacacctgc
    33601 aaaagctgca aacataccta caagaggatg tcttccaatt cctaaaaaca caagtgctgc
    33661 aagtggtggt aatattataa atcctacatc tgctgctgta tgtgataaca tacctgcaaa
    33721 tacgattgct acagttacaa gtttttgtgg aactttactt ataccctttt tcattaacac
    33781 ttccataaat ccagatttct cagcaacacc tgctccaagc attgtaacca gtactaatcc
    33841 taaaggagca aatgactgga agtttgtaac tatgctacct aaatatgatt gaatctgctc
    33901 tttctctagt agatttacta cctttacaac ttcttttgta gaaggatgta tttcttctac
    33961 tcctaaatta gatataactg ctgacaatat tacaacaatt atacatagta ataaaaatat
    34021 ggttactggg tctggaagtt tatttcctgc tctctctaca acatttagtg ccctcatcaa
    34081 taatccacta tctttgtttt tttcctgtga attaattttc ctcaaactaa taccccctca
    34141 taaactttat ctccagaatg ctttatctta aattttattt tttaatttat ccatataatt
    34201 tataacagac tgacatagta catttgtacc taccaatata gaatttttat cagctttaaa
    34261 tttaggatta tgccactgat atatacaatc ttgttctttg ttgcctaccc ctaaccagta
    34321 aaaaaatcct ggggcttttt ccatgaatat agaaaaatct tctcctcctg tagaaggtat
    34381 tggatcaact atagcatctt ctgaatacaa atcacacaca gacttacaag ctattgagtg
    34441 catctcttta ctatttataa cagctggtaa atcaaataaa taatctagct ttgcagttgc
    34501 ttgatatcca cgagctatct cacaaactat attttctaaa ttttctgata ttttctttcg
    34561 tacttcatta ctaaagcttc tacatgtgcc tgtcatatct accttttctg aaattacatt
    34621 atttgcagtc cctccattta tagtacctat tgatataaca gctgattcta atggacttat
    34681 gtttctgctt actatggttt gaatacccat tataattgca cttgatgcta ctatagggtc
    34741 tattgttctg tttggtatac ctccgtgacc tccaaatcca tttacttcta tcttaatagt
    34801 gtctactgct gccattaacc cacctaactt aacacctatt tttccacaag gtatatctgg
    34861 atgattatga agtccaaata tagcatcaac ttttggattt tccattactc ctcgttccac
    34921 tagcatcttc gcacctttat tgacttcttc ggctggttga aatataaact ttacatttcc
    34981 atgaagacta tctctaatat gagacaatac atatgcacat cctacaaggc aagacgtgtg
    35041 tatatcatgc ccacatgcat gcattatacc atcatatctg gatttatact taatatcaac
    35101 ctcttcatat attggcaatg catcaatatc tcctcttagt gctactgtgg gaccatcata
    35161 cttccctttt aataatccaa caactcctgt ttccattcct aaatctaata tttctatgtc
    35221 taaacttact aataaatctt ttatagcttt tgtagtccta aattctttaa aacttaattc
    35281 ggggtgcata tgaatatctg taaaaaaatc taaaatatct tctttatact tttctgagat
    35341 taaaataagt tcatgcatac taacacctct ccaaaatttt atttttttat tttttataat
    35401 ttaactacaa attatataag ttaataatct ttataaaga
//
LOCUS       DAELJO010000016        35004 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELJO010000016 DAELJO010000000
VERSION     DAELJO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.016"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(120..1523)
                     /locus_tag="KPB88_000739"
     CDS             complement(120..1523)
                     /locus_tag="KPB88_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0053041.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(1507..2034)
                     /locus_tag="KPB88_000740"
     CDS             complement(1507..2034)
                     /locus_tag="KPB88_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0053042.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            2314..2829
                     /locus_tag="KPB88_000741"
     CDS             2314..2829
                     /locus_tag="KPB88_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0053043.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            2878..3381
                     /locus_tag="KPB88_000742"
     CDS             2878..3381
                     /locus_tag="KPB88_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0053044.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSSFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            3378..4028
                     /locus_tag="KPB88_000743"
     CDS             3378..4028
                     /locus_tag="KPB88_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053045.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(4163..4942)
                     /locus_tag="KPB88_000744"
     CDS             complement(4163..4942)
                     /locus_tag="KPB88_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0053046.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(5054..5305)
                     /locus_tag="KPB88_000745"
     CDS             complement(5054..5305)
                     /locus_tag="KPB88_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0053047.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            complement(5634..6503)
                     /locus_tag="KPB88_000746"
     CDS             complement(5634..6503)
                     /locus_tag="KPB88_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0053048.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            complement(6841..7602)
                     /locus_tag="KPB88_000747"
     CDS             complement(6841..7602)
                     /locus_tag="KPB88_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053049.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            complement(7618..9153)
                     /locus_tag="KPB88_000748"
     CDS             complement(7618..9153)
                     /locus_tag="KPB88_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0053050.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            complement(9567..11807)
                     /locus_tag="KPB88_000749"
     CDS             complement(9567..11807)
                     /locus_tag="KPB88_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0053051.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            complement(12335..13135)
                     /locus_tag="KPB88_000750"
     CDS             complement(12335..13135)
                     /locus_tag="KPB88_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0053052.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            complement(13526..14401)
                     /gene="hslO"
                     /locus_tag="KPB88_000751"
     CDS             complement(13526..14401)
                     /gene="hslO"
                     /locus_tag="KPB88_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG0053053.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            complement(14686..15432)
                     /locus_tag="KPB88_000752"
     CDS             complement(14686..15432)
                     /locus_tag="KPB88_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0053054.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            complement(15419..15664)
                     /locus_tag="KPB88_000753"
     CDS             complement(15419..15664)
                     /locus_tag="KPB88_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG0053055.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            complement(15795..16913)
                     /locus_tag="KPB88_000754"
     CDS             complement(15795..16913)
                     /locus_tag="KPB88_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG0053056.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            complement(17119..18312)
                     /locus_tag="KPB88_000755"
     CDS             complement(17119..18312)
                     /locus_tag="KPB88_000755"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG0053057.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            18635..19516
                     /locus_tag="KPB88_000756"
     CDS             18635..19516
                     /locus_tag="KPB88_000756"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0053058.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            19941..20942
                     /locus_tag="KPB88_000757"
     CDS             19941..20942
                     /locus_tag="KPB88_000757"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG0053059.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            21059..21946
                     /locus_tag="KPB88_000758"
     CDS             21059..21946
                     /locus_tag="KPB88_000758"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0053060.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            22013..22762
                     /locus_tag="KPB88_000759"
     CDS             22013..22762
                     /locus_tag="KPB88_000759"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0053061.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            22986..23702
                     /gene="dapD"
                     /locus_tag="KPB88_000760"
     CDS             22986..23702
                     /gene="dapD"
                     /locus_tag="KPB88_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG0053062.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(24154..25143)
                     /locus_tag="KPB88_000761"
     CDS             complement(24154..25143)
                     /locus_tag="KPB88_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG0053063.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLIVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            25583..26338
                     /locus_tag="KPB88_000762"
     CDS             25583..26338
                     /locus_tag="KPB88_000762"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0053064.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTSVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPERENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(26845..28521)
                     /gene="bclA2"
                     /locus_tag="KPB88_000763"
     CDS             complement(26845..28521)
                     /gene="bclA2"
                     /locus_tag="KPB88_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG0053065.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(28899..29438)
                     /gene="hpt"
                     /locus_tag="KPB88_000764"
     CDS             complement(28899..29438)
                     /gene="hpt"
                     /locus_tag="KPB88_000764"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0053066.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(29766..31073)
                     /locus_tag="KPB88_000765"
     CDS             complement(29766..31073)
                     /locus_tag="KPB88_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG0053067.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(31498..33273)
                     /locus_tag="KPB88_000766"
     CDS             complement(31498..33273)
                     /locus_tag="KPB88_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053068.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(33270..33407)
                     /locus_tag="KPB88_000767"
     CDS             complement(33270..33407)
                     /locus_tag="KPB88_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053069.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(33485..34054)
                     /locus_tag="KPB88_000768"
     CDS             complement(33485..34054)
                     /locus_tag="KPB88_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0053070.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(34238..34873)
                     /locus_tag="KPB88_000769"
     CDS             complement(34238..34873)
                     /locus_tag="KPB88_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0053071.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT        11487 a         6060 c         4216 g        13241 t
ORIGIN      
        1 attaattttc tatactttct aaattagttt attatacttc tttagattaa ttttctacac
       61 ttcctaaatt aatttattat attttttaaa ttaattttct atacttctta aatattgtct
      121 tatacctctg caatcttatt atggttactc atcaaattat tttgctttat agtcaatgat
      181 ttcatttcat gaactgcaaa gtatatacat accattgccg ctgctgcatc tgctattggt
      241 cctgcaaaca ttactccatc aattcccata aacattggta aaattactat caatggaatt
      301 aaaaataaaa tttgacgagt caaagaagta aaaagtccca acactgattt cccaattgat
      361 gtaaagaaag ttgatgatac tatttgaaca ccatttataa atgttaagaa caagaaagtt
      421 ctaaaataat gttctgcaaa tataaaataa gtctcatttc cgttaccaaa tatagacgta
      481 atttgtcttg ggaataattg gaaacataag aatgcaataa cagatataat agttgcactt
      541 cccactgcaa acttatatgc ttcttttaca cgattatact tttcagcccc ataattatac
      601 cctataattg gttgcatacc ttgtgatata ccaataatta tagatataaa aatcatattt
      661 actttaataa tgataccgac acaagcaagt ggaatctcac ttccataaac tgacctagcc
      721 ccataatatg ccaatgtatt attcataact atctgtgtta cagtcattgc taattgatta
      781 aaacatgagc ttgaacctag cgcaaatata tgtttataaa ttatactatc taatttaaaa
      841 cttttcttat gtaatttaat atgttgaaaa ttctttatat aatgaacact tattccaaaa
      901 gatataagtt gcccaataac agttgctaaa gcagacccag caattcccat attaaaaaca
      961 aaaatgaata atggattcaa aattgtatta ataactgctc ctattaacat acaaaacata
     1021 gaagcttttg gtctcccatc tgcaagtatc aacttactca ttccagtgga cattataaga
     1081 aatggtaatc caattgatgt aatttttgta tatgtgtgtg cataaggtag tatttctgat
     1141 gtagagccaa agaattttaa tagtggtgtt aaaaatattg atactactat aaataaactt
     1201 attccaaaaa cagccatcaa gcatatagaa tttcctgctg cttttgtagc tttttctgaa
     1261 tttttagcac ctaattccaa tgaacactta gacgctcctc caattcctaa caataatgca
     1321 attgcagtac atattgttgt tagtggaaaa gtgacatttg tagcagcatt acccaataca
     1381 ccaacacctt gcccaataaa tatttggtca acaatgttat ataaagcact tactaacatt
     1441 gctataatac taggaattgc gtacttagtt aataactttg aaaccttttc atactccaat
     1501 ggctccttat atttttcttc cattttcctt cgcctccaat tttgcttctt cattcatatc
     1561 aatagccaaa aatgcaactt tctttaacat tgacataaac aactcttgct cttctttctc
     1621 aaatgaactc aacaactcct caacataccc atttgttatt tttagtattt catcataaca
     1681 tcctaaagat ttttctgtag gatataaatg ccatgttctt ttatccttta cacctggttc
     1741 tcttgttata aagccttctt tttctaaatt tgccaaagct cttgttatat tactaggatt
     1801 cacaagaatt ttctcaaata tagtgtcctg tgtaatacct ggattattac atatgtgtaa
     1861 tacataaaaa tattggctat tattgattcc aatatccgcc aatttcctgt caatataatt
     1921 tgtattgtat ctatttataa tggaaaccca ttttaatagc tctctcatat tctctccttt
     1981 aatatctatt gtccttattt ttttgcgttc gcaataatct atttgctcat gcaattattt
     2041 tactgctaat tttatacttt gtcaatagca cgcaagttta tatttaaacc ataaagttac
     2101 tataaatttt atatattaga ctcttacttt gatttaacta tttttaaatc ttcattgata
     2161 tttaattacc ttttatatta atttttttgc atatttatca ataactccta attcttttta
     2221 gcttatctta aaaagcatct tgacgtaaga tttattatct gtaataataa atatatcgaa
     2281 tcgatatata tctaattgat accaggaggt aatatgttag aatacattat tttaggattt
     2341 ttaatggaaa aagaattaag tggatacgac ttaaaacaaa taatgtcaga aagtacctcg
     2401 tacttttttg acgccagctt tggaagtatc tatcctgcac ttaaaagatt agaaacaaaa
     2461 ggatatatac actatcacga agttatagat ggtagcaaat taaaaaaact atattccata
     2521 actaatactg gtaaagaagt atttcttgaa tggttaaaga aacctataaa tttttctaaa
     2581 acaaagcaag accaccttgt taatatattc ttttacaagt atctacccaa agaaataatt
     2641 gaaacaaact taaaaatgtt aataactgaa gtagaattac ttctaaataa attaagtaaa
     2701 caaaaaaatg atgcagaaga aaatcattgt gttgaccaat atacatatat gtatgcaaca
     2761 atgatttatg gagttgacta ttataatttt gttattaatt ggtgtaaaga tttactgaaa
     2821 aatctataag atataatttt tagaagtaaa catacataat aaaaggagat ggactttatg
     2881 aggcttttaa tacatgattt aactgacaaa gaattagaaa agttattacc caaacttgat
     2941 gacaatataa aaatacttcc aaacacaaaa gaaatccaca aatgtattgg ttgttttggt
     3001 tgctggatta aaactccttc tgaatgtgtt gtaaaagatt attacagcaa catgggtaaa
     3061 cttataaatg aatctgatgg aattattata ataagcagat gctgttacgg agggtttagc
     3121 tcttttgtca aaaacgtact tgataaaagc attaattata ttcttccata ctttgtaata
     3181 aaagataata tgatgcgtca taaaagcaga tatgataaac agataaattt aaaagctata
     3241 ttctatggag attgtattac agaagctgaa aaagaaactg ctacgaaatt agtaaatgga
     3301 aatgctataa atttcaatat aaaagaacac tctgtttgtt tctatgaaaa tatacaagat
     3361 ataagaggtg aatccatatg aatatagtaa tgataaatgg tagtccaaaa gtaaataaaa
     3421 ataattcaca gtatttttta tctgaattaa aacaacttat tcaaaatgat aataaagttt
     3481 ttacatttaa agtagataac acatctaagt atcctgacgc tcttaatgcc atacttaatt
     3541 gtgatagatt aataatcgca tctcctcttt atgtagatgg tattccatct catttacttc
     3601 attttttaga gaaatgtgaa aaatttttaa aaacacaaaa taaaaaacct attccagtgt
     3661 atgcaatagt taactgtgga ttcaatgaag gtgaacataa tcatttggca attcaaatga
     3721 tagagcattg gtgtaaaaaa atcaacttta catgggttca aggtattgga attggagctg
     3781 gggagatgtt tggttcacta gaatccgtac ctttgggtaa aggacctaaa aaagatttag
     3841 gaattgcatt aaaagaactt tcaaaaaata tacttagtga taatatacat atagatgatt
     3901 ccaacaaaag tactattttt atttcaccta acttccctag attcctatat aaacttatgg
     3961 ggaatcatca atggaatact caagctaaat caaatggatt gactaaaagt gatatactaa
     4021 agaaataaaa ttttacttca acaaatacta aaaaccgtca agcacgaata aactattcat
     4081 cttgacggtt ttttatgtta tttatattca ctagacatgg gataattcac tataacattg
     4141 attatctagt ctaatattta tcttattctt tttgtaaaat ccaagttgct ccaacaaggt
     4201 cctgctgtaa atcagtatat tcattttttt ggctaataac atattgttca aacatatctt
     4261 tttgagaagg atatttttta attagctcat ttgcacgagt tataatccag ttcatttcct
     4321 cttcaacatc attttcaagt tctaaacatg gtatacattc aattagtttc agacctaatt
     4381 ctttgaacaa tatacaccac tcattgtaag tgggatacac cctctcaaaa ttaagctgtt
     4441 catttgtaga tttatctact acatatgcat catcaattaa aatgaacccc ttacttttta
     4501 ttgtaggtaa tagcttactt atagtttctt tataattacc taaaatatcc cctacagctc
     4561 caaaaatcac acaatcataa tcatgctcaa tttttacaga actattagca tcattcattt
     4621 caaaatcaca caaagcatca accttatatt caatagcctt tcttctcgca taatcaataa
     4681 attcaggtat caaatcaata cctttgatat gtatattcaa ctcttttgca agcttcacag
     4741 atacagcacc ttttccacaa gctaaatcca aaacttttgt atcttcatta aaaggtatat
     4801 gctttctaaa taattgtaac atttgttctg gtgaacttcc taattcccaa aaatcttgta
     4861 ataaataagg caaataaggt aaaatatcag tagtatcaga agttagtgac tctgccaatt
     4921 tttcttttac agtatcattc attttttcaa cctccaaaat ttaaattatt attcaaaagt
     4981 aatattattt tatatacttc ttcataggta ctacaaacag tggaaaatgc ttttgacctt
     5041 tgtattttgc aattcatttc ttttctttat gagctgtaat aattgtataa ctgtgagcgt
     5101 ttactgttat aatacaatca tatacactta tataccagtt ttttcccttt ctattaataa
     5161 tggcatttgg agattctatt ttagttttac accagttcac tacatcattt gtctccaaag
     5221 aaagattttt cttaattctt acaacgccta actctgttgt atgcaatcta tctaggtttt
     5281 taagtaattc gttttctaca ttcattttta ttaaaacacc ttatcctttg caaaattaaa
     5341 tctattattt agtactattt tatcatagta ttgtgtattt tatcataatc ttatagtgaa
     5401 actatttctt tcctacagat tataaattat ttatgaatac acaccattat agaacttttc
     5461 ctatgcaaaa ttaaatctat tatttaacac tattttgtca tagtattgtg tattttatca
     5521 taatcttata gtaaaattat ttctttccta cagactataa attatttata aatacatact
     5581 gctgtaaaac tttgcctata taaatccatc tactttatac ataatacctt cttttaaatg
     5641 attggaaccc aaaactgaat ttcactcaaa ccattctcat cagttttttc tccatatttt
     5701 acaaagtcat ataaattatt ttcattaaac tggcaacttg aattagggaa ccattcttga
     5761 tagatatatt ctacaacatt ttgcatacta tcctcatttt tcccaataaa agtaaaaaca
     5821 atataattac ttttcggtaa tacgaatttt tccattccat taggtaattt gccaaaagta
     5881 gctacttgag cacctgcaat gtatgtaaat gtaggagtat tctcaatctc tttaaaattg
     5941 ctataatcac taactccaat taaaaaacct aaatctacct tattttctat catctcttta
     6001 tttttatgca aatccctcca gcattttcca ataatatgaa atccttttct tactttacca
     6061 gaatagccaa caatatttat atctgcacaa ttaacttttt taacatctaa aaccatatct
     6121 ccacgtaatt tttttctatt atgaatatca aattttaatt gtagtggtaa aaaatcttga
     6181 tgtcttctaa aaaaattagg actacgttta aacatttttt taaaccctct tgaaaaagag
     6241 cgttgtgtat cataccccga gattaaagca atctcaataa ttggtttttc tgtaaaaatt
     6301 agcattctcg ctgcttcagt aagtctacgt ctttgtatat aattatgtac actaaatccg
     6361 acaactgatg taaacattct atgtaaatgg tattttgaat aacctatctc tttagaaatt
     6421 acatttaaat ctaacatctc atctaaaaga tgtttttcta tataatctaa agttttttca
     6481 atggtaatca aattgttatc catatagcac ccctcctatt ttaaagatag catattattt
     6541 tattttgttt ttcacattct ttgctaatat tttgatttat tttatggcac acaaaagaca
     6601 cttctatttg aagtgcctat ctactctgta tattgagctt cttatttcaa tttattataa
     6661 tcttcaacag atacaggttc tagccattta ttgaaagcac cttcagcagt tacctcgtct
     6721 acagccaact gctaaatgcc cttctatacc ttaatctaac ttttccactt caagtataca
     6781 acttgaagtt agctctaaat caatagtttt tatcacaaca atttgatttt aaatttaaat
     6841 ttattctttt aaattatcat catctattat agattttctt atattcaata actggtctac
     6901 aaattggtta gcaggagtat ttaaaatatt ctcaggagta tcatactgtt gaacaactcc
     6961 atgatttaat accaaagtct ttgttcccaa aatcaaagct tcataaatat catgcgtaat
     7021 aaagataatc gtaactcctg tttttttatg aatttctttt atctcttttt gtaattgctt
     7081 tcgtgtaatc tcatcaactg caccaaaagg ttcatccatt aataaaattt ctggagaaga
     7141 agctaaagct cttgcaatcc ctacacgttg ttgctgacca cctgacaatt ctgatgggta
     7201 acgcactttc atatcatttg gcaatcccac tatttccatc cacttgttta cagcactttt
     7261 tacttctaat ttattctttt tacttaggag atttggtaca tatgaaatat tttcttcaac
     7321 agtcatatga ggaaaaagaa cacttccctg tacacaatat cctattcttc ttcttaattt
     7381 aattaagtcc tcgtttttga tatctgtttg gtttatcaaa atatttcctc catctggcaa
     7441 aatcagacca ttaatcattt ttaagagtgt tgtcttacca gaaccagacg aaccaattac
     7501 agtcaaaaac tctccttttt ctatatctaa attaagatta tctattatag ttttatcatt
     7561 atattgtttt ttaatatttt taaattgtat aataggtgtc attatatttc ctccaattta
     7621 taaaagacct ttcttattca gaaactcacg agcaacttct ctaggttcta aattgttgtt
     7681 ttctacatca taattcattt tagccatttc tttatcagtc aaaatatctc caaccatctt
     7741 aaataccttt gatagttctg gattttcctc taatacttcc aaacgaacaa catttccaca
     7801 ttgataagat gggtagaaat ttttatcatc ttttagaact acaacatctg aaacactata
     7861 ctgaccatca gttgtaaata taggcattac gtcaatctta ccttcattga tagcttggta
     7921 ttttaatcca atatctaaat caactgtatc tttaaagttc agtccatatg tattacacaa
     7981 tggagtatat ccatcctctc tctcataaaa gtcatattca gctccaaatg tcaatttaga
     8041 agatacatct tttaagtccg ttatattttt taaattgtat ttatctgcaa cttcttttct
     8101 aactgctaat gcatagctat tagaaaaacc atacatacca gtccattgca ttttaagttt
     8161 agttttgtat tccgactgta gtttactaaa catactttca gtatataatt catttttctt
     8221 aagtaccata ttccagcctg tacctgtata ctctggataa atgtcaaatt ctccattctc
     8281 catagctggc tggatattag atgttccccc tcctacacct tgtgtaattt ccacgtttag
     8341 acctgtgttt ttttcaatta aaatatccaa catctcacca agaatatatt gttcagtcat
     8401 aggtttagta gcaatatgaa tagtttgagt ctttgaagga ttaaataaat aagacgtaaa
     8461 gatagttgct ataagaataa ggcttcctag aatggcaaaa acttttttcc tatttttctt
     8521 ttttcttttt cgttgaaaaa atttttctac cagccctaaa ataaaatcaa atattaaagc
     8581 taatagagca ataagtatac ttccagtaat tgtcattatt gtgttatttg tcgtgattcc
     8641 tctatatatg gctacaccta gacctccagc acctataaat gaagcgatac cagctaatgc
     8701 aattgtcata atcaccatat ttctaatacc agacataata acaggtaggg ctaacggaag
     8761 ttttatttta tacaatatct gaaaatctgt acttcccatt cccctagctg cttcaagaat
     8821 atttttatcc acattagtaa ttccagtata tgtatttcta accattggca ataatgcata
     8881 aatggtaagt gcaataacag cagtagtatt tccaattccg gaaaatggta tcagaaatcc
     8941 tagcatagaa atagatggaa tggtgtatag aaaattgata gttcccaata taggtttaga
     9001 agaattatga aattcactta caataatacc aattatacca cccaaaacaa tcgcaattat
     9061 tatagaaata atagaaatca tcaaatgttc tatggatagc tttataaaaa aatcacttct
     9121 ttcagataat aaggttagaa tatcatttat catccatctc tctcctttta taagttttac
     9181 ttaacaagat ttaaatttta atgtagttaa tctttattgt ttatacatca gcaaatcgtc
     9241 atcaaattat ttcacaaatt atatattaag attataatct gaacctccca tgactgaagt
     9301 cacaagtgtg tatagccatt ttttaatcaa acttgcaagt agacacaaaa aagtaactat
     9361 tagagactcc atcctcgata ttctccataa aaataagaaa aaaggtcaga accgttaaaa
     9421 tactaaaatt taaacaaatt tctgacactt tatttaatta aatttgaata tttctcacat
     9481 tatttattac tgttaagttt aattatcatt ttatataccc attatattta ttaaaatatc
     9541 attataattt tattatattt tatttattat actatatatt ttgctgttaa tgatatttta
     9601 ttgttacata attgttttgg aataccttca aatattactc tcccgccttt tgttcctcct
     9661 tcaggtccca gctcaataat ccaatcagct cttttaatta catctatatt gtgttcaatt
     9721 attatgaccg tatttccatt atctacaatc tcttcaatta tacttataaa attctcaata
     9781 tcagccatat gaagacctgt tgttggttca tctaatatat acactgaact ttccttattt
     9841 aattcactag caagctttaa tctttgacac tctcctccag acaaagtatc aagagtttgt
     9901 cctaaagtaa ggtatccaat acccattttt tcaatacatt gtaatttcgt tttaatctgt
     9961 tttaaattaa aaaactcaat tgcctctaaa acagacattt caagaacttc aattatattc
    10021 ttcccttgaa acctatattc taaaacttct tttttaaatt ttttaccttc acaaacattg
    10081 caggtactct ttatactctc cataaaagca agattcattt ctactattcc agaaccctta
    10141 cagttatcac aagctccttc tgaatttgaa ctgaataaag aagcatttac gccatttgtt
    10201 tttgaaaata tgcttcttat gttgttcata attccactat atgttgccaa actcgacctt
    10261 gaatttgctg aaacagggct ttggtctata agtacagcac taggattttg ctttaaaaat
    10321 tcatccttaa tcaacgtact tttacctgct cctgctactc cagtaactag agtcaataca
    10381 cctttaggta tcttaatcga tacatttttt aaattgtttt tattacaatt tttaacttct
    10441 aaatatccac tatgttctct tacactttct ttaataggaa gaaatttact taaagcattc
    10501 cctgtcaaag taccacttgt aagtagattt tcataactgc cttcatacac aacttctcca
    10561 ccatacttac ctgcctttgg tcctacatct acaatatggt ctgcaatctt aataacatct
    10621 gggtcatgtt caacaactat aattgtattc cctttatcac gcaatttttt taaaagatta
    10681 tttaatttat acacatcttt tggatgaagt cctacacttg gttcatcaaa aatatacatt
    10741 aaatcaacaa gattactatt taaatactta accatcttta tacgctgaga ttcccctcct
    10801 gatagagaag atgtctctct atccagtgtt aaatatccaa gacctatatc tatgatactg
    10861 tttaactttt ctattattcc tttaattaaa ggttgtgctt ctggctcatc tatactttta
    10921 ataacttcag ccaattcatc aatttgcata ctagttaaat ctgctatatt ataaccatta
    10981 attaaagact tatatactct ttcattaagt cttcttccat cacatatagg acatctatct
    11041 tctataagaa gtttactcaa ttttttctta gtagcttcag atatttctcc ctctctactt
    11101 aagtataggc gattaaattt aaccattagt ccctcatatg tcatgttagt ttgaccagca
    11161 ttttctatct taatcttttc tgctttacca tgcaaaaact tttccaattc tacttctgaa
    11221 taatcacata tcctcttatt aatatcaaaa aatcctgatt cagcgaatat attccaatgc
    11281 caagaaccca ctccaaaccc tgaaagcaat atagcacctt catttaatga tttattcata
    11341 tcaagaattt ggttcatatt aggaactagc ttttttccaa gcccatgaca gtgtgggcac
    11401 ataccatcaa tattattaaa agaaaacata ttggcattac ctatatactc actaccaaac
    11461 cttgaatata aagctcttaa aaaggaattt acatctgtaa tagtacctag ggttgacctt
    11521 gaatttccac caagtctact ttggtctatg ataattggtg ttgacaaatt ttctatacaa
    11581 tcagctttaa cctctccata cttaggtaaa aaacttctta caaatgtact aaacctttca
    11641 tttagttggc gttgtgattc ttttgcaatt gtctcaaata caatagatga ctttccagaa
    11701 cccgacaccc ctgtgaatat ggttattttt ctttttggta tttttaagga aatattgttc
    11761 aaattgttct ctttgcaacc ttttaaaatt ataaattcat tgttcatatt ttacctcctg
    11821 cattattttt ctttaaatta tttagagaaa atcgtaataa tagttgttag ttatacataa
    11881 gtgaaatact attttattag attactcctt tttttataaa cacaggtcaa attatagcat
    11941 gtagatttta tctggtagta tagtgtaggg tttggatttt tataaaaaat acagttattt
    12001 tatttaagcg taattacatc tattctacct aaatatcatc attatatatg agtgttaaat
    12061 tatataaaat tcttaagtta ataatctcaa aatggtatta gttacataaa aaaagtatct
    12121 tgcctatgat tttaaaacca taaataagat actatgtttt acactgtttc ttctttcaca
    12181 aataattcta tacaattatt caacttaact ttaaaatttt aaacactaaa aacctctatc
    12241 tttaaatctt taaataaaac taaatttcca ataccttcaa aactatagaa aattccaatc
    12301 aacttatcaa acataactta ctacttataa gaaattaatc ttcacttaat acagacgtaa
    12361 gttcagggtc atttagaaga tgttcaaaat aactatgtgc cgaaatatat cctataccat
    12421 acattttacg ctttgaattt acaaccttat attcataaca agcactctta accttattgt
    12481 agtcatcaaa atccattgct ttatagatag ctctttcatt tcttggtata tactttacat
    12541 attttggctt aacaaaatac aagtcatctt ttatcttacc ctcacgttta agtttcttta
    12601 aaacttcccc attctttcta tgcattatat ggtcatcata cttatagtga tgggctatat
    12661 gggtcacatt gcctttaaac ttatgttcaa aatccaatat agtcttttcc attaattcat
    12721 aatgtgtgcc ttccttttta tctatatctg caagtataat cacattttga ggtttcactc
    12781 caagctctct caaagctgat ttaaactcgt tatttctaag ctcttccttt tgtttcctag
    12841 ttaaatttct aaattttgtg ttagctttga aaacgttaac tccagaacca tctgatacta
    12901 ataccacata tacattatca acaccacact gttttatagc atctacaatg gcacttcctc
    12961 cccataaaac ctcatcatct tgatgttgag gataaaacac tgctgctgat ttaaattttc
    13021 tactattact ctcaggaata ggtatttcaa gttttccgtt aaaattcttt gcaattaaaa
    13081 atatcatcaa taatattaat atacttattc ctatgtactt cgttttactt tccatattta
    13141 aaattttctc cttcttctga acctcccatg attaaagtca cagggttact acttcataga
    13201 atttcgtata atcaactcaa atatcttata aacatttgtg tcaatcaggt ctaaatatac
    13261 aaattatttt acaatatttt attgtttcat acaatctttg acacgaacta taataaaata
    13321 aaaaagcctt ctaactgcaa cattgcaaac attacagcca gaaagcttct taattcttaa
    13381 aatttattaa gcttaaaaat ttattaaact tattttttaa tataaatatg tctattatta
    13441 ataaattata ctttaattac tatatcaata cgactttact atatcactat aacattactt
    13501 atatcaatac aactattttt atagtctaca tttcctttaa aatttcaagc aactcttctt
    13561 cactatacat atacttctta ttacagaact ggcaaccaac ttctgctttt ttatcttctt
    13621 ctattatttg agccaatgat tttttaccaa ttgcaaccaa agcagttttt actctttctt
    13681 ttgaacattc acacataaac ccaacttcac aggtatcaag tattttaggt tccaaaccat
    13741 ccaaaacaat atttaatata tcctctggtg attttccttc ttttaacatg ttagttatag
    13801 aaggtaattt agctacattt tcctctaact tagcaatagt ctcttcttct gcatctggca
    13861 ttaattgaac tataaatcct ccagcaaact ctacttcatc ttcttttgta agaacaccta
    13921 aagctactac tgatggagtc tgctctgata cagcaaaata ataagtaaag tcttctgcaa
    13981 tttctccact taccaatgga taagtcccat tatatggttc tctaagacct aagtctttta
    14041 taacttttac aacaccttct gtacctactg ctgctgctac atttaacttt ccatttggat
    14101 agtcttcaac ctctacttgt ggatttccta catatccttt taccattccc ttagagtctg
    14161 atgtggcgat aatagttcca attggacctc caccttttat tattacagtc aatttatcac
    14221 tatcattttt catcattaat cccattatag atgtggctgt taatgttctt ccaagtgctg
    14281 ctgttgctac ttttgtagtt tcatgtaatc ttctagcttc ttctactgta tttctagtag
    14341 ttgcgacaaa tgctctaact tgaccatttc ctgatgtcgc tcttagtaca taatctctca
    14401 ttaatattcc tccatgtccc gttaattgtc ttatttttat tatattacta ccttaatttt
    14461 aatctataaa tacttatcta tcttaacctt gggcaaaacc cattttattt taattttact
    14521 aagcaatagt tattatattt taatttttat ctataaaact ttataattat tagaaaataa
    14581 aaatcataaa atatcatcac tttactttta atctataaaa ctgcatttta tatattttta
    14641 tatcatggtt atttttaata attatcttat atagtcaaga atatcttatt ttttacatac
    14701 aaagaagatt ctttgacttt cttcactagg tttgtcaaat gtaaaatccc cataatattt
    14761 tatatcctta aatccagatt tttcaagcat atcaactatc tcatcttcag tatatgctct
    14821 ttgttggtgt atctcttcaa atctttcaaa ctttccacat tcatctttaa taaagaatgt
    14881 aagttccatt tcaattagat tctcttcttc atcaaagtaa ttttgccaca tatacgctat
    14941 atcttctcta ttttctccat acatgttatt tcccaatatg ttggctaatt tataaaaaga
    15001 acttatatca aatatgaata ttccatcttt ctttaataat tcatatgtct ttgaaaatac
    15061 attttccaaa tcatcatcat aagttatata attaaatcca tcacaagcac aaagcacaca
    15121 gtccaaatca gatatttcaa aatcaagctc tgatatatct tgttgaagta aaactaattc
    15181 tactccttct ttttctgcct tttctcttgc tacactaagc atctcatcag atatatctat
    15241 accagctata tcgtaatttt ttttagtcaa tggaattgta agattacccg taccacatgc
    15301 caattccaat atatttttta cttttacacc ttcactatct attatatctt ctatatattt
    15361 aacccaacca ttataatcaa cttcattcat aagttcatca tatacaaacg caaaatcact
    15421 atattgactc atccttttta ttctgctcct ccacaatctt tttaagcctc tttttgcgtg
    15481 atgtaagcat acctccaatt cttcctgctt ccttagctgt aagtcctgcc catccacgtt
    15541 caccaacttt atccataaga cctagttctg aggctatctc atatttcatt atatcatcat
    15601 ttgtcaatat tttagtattc ttgccattag ttatcttctt ttctctttgt actttttctt
    15661 tcaaaaaaat caccccttct tatttgtatt tttgtcaatt aaaataaatt tatacaaata
    15721 tgtaaggggt atttattaat acactttttt taaatttagg tatttatgct ttttaatctt
    15781 tataacttat cttcctaatc tattgcttct ttgtatttta ataattttga ataaacattc
    15841 aatgcattta cacatatctt ttcatcaaaa ttaaagttta tattatgaag accatttaca
    15901 aatccttttt cttcgtttct tgaacccaac ataaaaaata atcctggaac ttctttttga
    15961 taataagaaa aatcttctga tatcataagt ggctctaact ctacaacagt atcacttcca
    16021 atagcctcta caaactcttg atacaaatcc ttgtcatttt taacagcgat ataatcatct
    16081 ataatattta tattaacctt acagttataa gccaattcaa accctctagc aagttctcta
    16141 actctaagct tcattttctc atacacttct gggctgaaac atctcatagt accttccatc
    16201 tttatatttt cagctattat atttcttcta gctccccctt caattctacc aatagtcaac
    16261 actgcattgt ctattgggct tatatttcta gacactatag tttgaagtga gcttacaaag
    16321 tttgaagcta tcactatacc atcaatacca ttttgtggca tagctccatg tccactttta
    16381 gacactattt ctatgtcaat atctcctatt tgagccataa aatagccctc tcttgtacct
    16441 acataacctt ctggtaactc tggatatata tgaagaccat atatttcatc aatattgtat
    16501 tttctaagca ctccttcatc tattaaagct tttgctcccc caggaccttc ttctgccggt
    16561 tggaatataa atactatatt atcatttaag tcttctttat tatcatttaa atactcaatt
    16621 aatccaagta agatagtagc atgtccatca tgaccacata aatgttttac cccttcttca
    16681 ctatgaagac catctatgtc agctctaaaa gcaatatctt ttttaggtct tttaccttct
    16741 attacaccta ctatacctgt ttctaaataa acttcatact caattcctaa tttatcaaga
    16801 taatctctta tatattttga agtcttatac tcttttctcc caatctctgc aataggattt
    16861 aatgacttcc tatgtttata aagttgttct tgtaaatatg atgcctctct catgtaatct
    16921 cctccaattt ctaatttatt aaaaaatatt tctatatttc tttataatta aaatattata
    16981 tagcatgtta tactaattaa aaataaaaag ctaaattgaa tataaaaata ttttgcatta
    17041 ttaaataatt caccattaat aataattccc ctgtttcata aatcaaaaca gaggaattat
    17101 tattggaaaa ttttattttt agcaagagaa gaaatgttta gcaagtccac ccttagctgt
    17161 ttctttgtac ttatccatca tatctatacc agtttctttc attgtttcaa caacttggtc
    17221 taaggagaca gaatgtgagc catcagttaa taatgcataa ttagctgcat cataagctct
    17281 ttgagctgcc attgcatttc tttctataca tggaatttga acatatccat atacagggtc
    17341 acaagtcatt cctaaatgat gttcaagtgc tatttctgcc gcatattcaa tatggtctat
    17401 agttccacct tttaagtatg ctactgctgc tgctgccata gaacaagcgg ctccaacttc
    17461 accttgacac cctacctctg ctccagatat tgaagcgtta gtttttatta tattacctat
    17521 tattccagct acaagtagtg cttcaattat tttttcgtca tcataattat agaaatcctg
    17581 cattgcaaag aatattcctg gtattactcc agcagaacca caagttggtg ctgtaactat
    17641 tatattacca gaagcgtttt gctctgaagc tgctagtgca tatgcatata caagtgttga
    17701 gaagctctta tcttttttat atgcattata aaatgtactt gctcttcttt ttaataataa
    17761 cttgcctggt attatttcat ctgtacttag tccatcatca atagattttc tcatagcatc
    17821 ttttatagtt tttatataat cttttatgtc ttttggttca cattccaaca caaaatctac
    17881 caaagtctta ttattttctt tgcaccattt tactatctca tccatagtat ctaagtgata
    17941 tatactattg cttttactgt ttctttgacc ttcttctgct atagtccctc cacctacaga
    18001 atatacagtc cattcagctg ttacattttt atctttatct aaagcttcaa acttcatccc
    18061 atttgggtga aaatctttta taatatcttc ttcccaaaca atttcgactt ttttaggtgc
    18121 tatagttttt tcaattatat agtcagttaa atgacctttc cctgtggctg ccaaactacc
    18181 atataatatt gctctaaaac tttctgcatc tgggttttca ttcttaaatc tctctgctgc
    18241 tctttgtggt cccatagtat gagaacttga aggtccactt cctattttaa aaagttcctt
    18301 taaagtatcc atattattcc tcctaaattt acggtattat tgcttattgt tagattttac
    18361 gcacatattt attataacct aaatatatta attattagtt attaaattct cataaaattt
    18421 attttttatt tccattttgg tatattttat taaattaata tgcatatttt gagaataaaa
    18481 catttattta aaatttttct aatattaata taatataaat atcttattta aacagttaag
    18541 atattaacta tacctattaa aattaattaa tttgtactat aattattatt gtacttaaaa
    18601 aattaaattt agataaaaaa ggagatttga ttatatgtta tttaaaggtt ctgctgttgc
    18661 tttagttacc ccatttacag aggataataa tgtcaacttt gaaaaattag gtgaattaat
    18721 cgaatatcac attgagaatg gcacagatgc actagttgtt tgtggaacaa caggtgaggc
    18781 gactactatg agtgaatctg aaatttttgc tgtaataaaa tatacagttg aaaaagttaa
    18841 taagagaata cctgttatag caggtactgg ttcaaacaat actatgttgt ctgttcatat
    18901 gagtcaagaa gctgaaaaat taggggtgga tggtctttta ataataactc cttactataa
    18961 taagactaat gagaaaggtc ttaaacttca ctttgaaaca atagcaaata gtgttaagtt
    19021 gccaattatt ttatacaacg taccaggaag aactaaagtg aatataaagc catctgttgt
    19081 ggccgaactt gctaaaatag ataatatagt tgctgtgaag gaagcaagtg gagacttagc
    19141 tcaagttgca gaaatagcta aattagtacc taaagacttt gctatatact ccggtaatga
    19201 tgatactata ctaccactat tatcattagg tggaagtggt gttatctctg tacttgctaa
    19261 catatgtcct aaagaaacac atgatttagt aactaaattc tttgaaggtg atattgaagg
    19321 ctcaaaaaaa ttacaacttg atatggatgc tttaattgct gccttattta tagaagtaaa
    19381 tccagttcct gttaaaactg ccatgaacat tttaggtttt aatgttggtg atttaagact
    19441 tcctctagct gaaatggaag aaactaatct taatgtgtta aaacaagaat tgacaaattt
    19501 tggatttaaa ttttagcaat atttatataa ataagttgat tttttcttaa atatgtatta
    19561 ttattagaat aatcaaattt taatatccta ttaacctagg gtagaggtag ctgtaactaa
    19621 gagtatccaa tagtagctgg caggtgttaa tgattggaaa aaggatttac agccgaagaa
    19681 aatatttctt gcaataatat tttctgggat tgcacataac atgtgtaata ctgtcactta
    19741 tttttgtagg tgatgagcta cagggtttac tttgagtgat aaattagacg tacaaagtat
    19801 acctttgtac gttttttgta tataattaaa gttgttaata taatattaaa gtttttggat
    19861 atataaaaat attaacatat cttacttgta taactaaaat aaaataatgt tttaataatt
    19921 taaaattggg gtgaatttat atgaaaaaag taaatgtagc aatagttggt gctactggaa
    19981 tggtaggtag aacattttta aaagtattag aagaaagaaa ttttccaata gaaaatctat
    20041 ttttattctc ttcttctaaa tcagctggtt ctaaagtaat gttttgtggt aaagaatata
    20101 tagtagaaga attaaaggaa acttcattta ctgatagagg catacaaata gcattattct
    20161 ctgctggtgg aagtattagt gaaaagtatg ctccaatagc tgcttcaaat ggaatcttag
    20221 tagtagataa ctctagtcaa tggagaatga atccagatgt gcctttagta gttcctgagg
    20281 ttaatccaga agccataaaa aatcataagg gtataatagc taatcctaac tgctctacta
    20341 tacaagctat ggttcccctt aaagctcttg atgataaata caaaattaag agagttgttt
    20401 actctactta ccaagctgta tctggttctg gtgttaaagg tgttgaagat ttagagaaag
    20461 gaatgaacgg taatcctact ggattctacc ctcatcaaat ctcttacaac tgtcttcctc
    20521 atatagattc attcactgaa aatggataca ctaaagaaga aatgaaaatg gtaaatgaaa
    20581 ctatgaaaat acttgataac tacgatttaa aaataactgc aactactgta agagttccag
    20641 taaaaaatgg tcatagtgaa tctataaatg ttgagtttaa caatccattt gagttagatg
    20701 atttagtaaa aacacttgaa gaagctccag gtgttgtagt agttgataat cctgctaaaa
    20761 acgaatatcc aactgctgta gaatttgatg atagagatga agtatttgtt ggaagaataa
    20821 gaagagattt tagtgttgat aatggtgtaa atctttgggt tgttgctgat aatataagaa
    20881 aaggtgctgc aactaacgca attcaaatag ctgaaatggc tttatcatac gatttagtat
    20941 aatatacaaa cttgtataaa tatataattt tgtaaaatat taatagtaac tcaaaaacaa
    21001 gcattagaag taactgtaat ataaaacaca aaaataaaaa agaggggatt gattttatat
    21061 gatatttaaa ggttctgctg ttgctctagt tacaccattt acaaaggata acaaagttga
    21121 ttttgacaaa ttaggtgaat tagttgaata ccaaatagct aatggtactg atgctatagt
    21181 atcttgtggt actactggtg aagctaatac tatgactgat gaagaacaac ttgctactat
    21241 taagtatgtt gttgaaaaag ttaataaaag agttcctgtt atagctggaa gtggttctaa
    21301 tgatactatg cactcagtta acttaagtca agaagctgaa aaattaggag tagatgctct
    21361 attaataata acaccttatt acaataaagc taataaagct ggacttaagc gtcattttga
    21421 aacaatagct aacagtgtta aacttccaat tatactttac aatgtccctg gaagaacttg
    21481 tgtaaatata agtcctagcc ttatagttga acttgctaaa attgataaca tagttgctgt
    21541 aaaagaagct agtggagacc taggacaggt tgcagaaata gctagtttag ttccagatga
    21601 ctttgctatc tactctggta atgatgatac tatacttcct ttattatctt taggaggtca
    21661 aggagttata tcagtacttg ctaatgtttg ccctcaagaa actcacgatt tggtagctaa
    21721 attctttgaa ggcgatattg aaggttctag aaaattacaa cttggtatgg atgctctaat
    21781 cgcagcttta tttatagaag taaatcctat accagttaag actgcaatga atttattggg
    21841 attcaatgtt ggagatttaa gattacctct agcagaaatg gacccagcaa atttagaagt
    21901 gttaaagaaa gaattagtta actttggact aaaagttaat gcatagtaaa gattaatgca
    21961 tggtaaaaag taagtttggt caaaagttag cccttaatca taggaggaaa ttatgttaaa
    22021 agttatagca actggttatg atggaaaaat gggtaagatt ttagccgata ctataagaga
    22081 agataatgaa ttagaattgg tttgtgttgc agctagaggt ctggatagct atgaaggcga
    22141 ccttaaaatt tatgaagata tgagtactat agttgaggaa gctgatgtag ttatagattt
    22201 ttctcatcac tctaatttag ataatatctt atcttacgtt ttaaagacta aaacaccttt
    22261 agtaattgct actactggat ataatgatga cgaaatgaac aaaatacgtg aagcggctaa
    22321 agttattcct gtattccaat catataatat gtctcttggt gtaaatgtat tagtaaaatt
    22381 ggttaaagat gcagctaaat tactagaagg atttgatata gaaataatag aaaaacatca
    22441 caacagaaaa gtagatgccc caagtggcac agctgttatg attgctaacg ctataaaaga
    22501 ggttttgcca aacgttgaaa ataattatgg tagatatggt cgtagtgcaa aaagacaacc
    22561 aaatgaagtt ggtattcatg ctataagagg tggaaatatt gttggcgaac atgatgctct
    22621 atttgctgga gacaatgagg ttcttacaat aagccatcaa gctcaatctc gtggtatctt
    22681 tgcaagtggt tctatagctg ctgccaaata cttagttaag caaaaaccag gattttataa
    22741 tatggacagc atgttgggat aatagtataa atataataat acacaatata aattaaacat
    22801 atacaatata gatataataa tacgcaatac aaatcaaaca tatataataa atttatttaa
    22861 tactatgtct atacaataaa aactaactaa aataaaatat aaataataag aagtaagtat
    22921 atactaaaat tgacaaataa gtatataata aaagaaatat aatgaaatac aaggagaatt
    22981 aacaaatggt aaatttaaac gatgcttatg aaatagcaag gtatataaaa gaagttaaaa
    23041 agaaaacacc tgttaaggta tatgtaaata ctaactcttt acacttccaa tcaactgata
    23101 aatttaaggt atttggaaat aatggttctt atatatttgt aggtgactat gaggatataa
    23161 tggaagtatt agataaacat ggaaatgata taacagatac tcatgtagaa tatgatagaa
    23221 gaaattctgc tattcctcta aaaaatactt taagtgaaca tgcaagaatt gagccaggtg
    23281 caataataag agacatggtt actatagaaa aaaatgctgt tgtaatgatg ggagctgtta
    23341 taaatatagg agctgttatt ggtgaaggtt ctatggtaga tatgaatgct gttattggag
    23401 ctagaggaac tcttggtaaa aatgttcact taggtgctgg tgctgttgtt gctggtgttt
    23461 tagagccacc ttctgctact cctgttatag ttgaagatga tgttttaata ggagctaatg
    23521 cagttattct tgaaggtgtt agaatcggta aaggtgctgt tgttgcagct ggtgctgttg
    23581 taactactga tgtagaagct ggtgctgttg ttgctggttc tcctgctaaa gttataaaaa
    23641 tgaaggatga aaaaacagct gacaaaacta agcttatgga agaccttaga aatttagact
    23701 aattaaatag tactatacta ttaattaaac aataatatac taatttaaaa aattaattaa
    23761 aaaggtgtct ctgtgtgaat ttaattcacc agaggcaccc tcttttttac aatattttta
    23821 tcttttattt ataatcattt tattttttta caattctctt attcattctt atatcatttt
    23881 tatttcttgc tgacaagtat ttttatttcc ttcaattttt caatatatca atgtaataaa
    23941 ctattaaact ttttataatt taataaagtg aaaaccatta atctttctat aatttaataa
    24001 aatgaactac taaattttct atatatcaat agaataaatt attaaacttt ttataattca
    24061 ataaagcaaa ccattaatct ttctataatt taataaaatg aactactaaa ttttctatat
    24121 atcaataaaa taaactatta aactttttat aatttaataa atagaactgc tgtttaccac
    24181 tggttgtgca tctttattgc cacaaagcac aactaacaga ttacttacca tagctgcttt
    24241 acgttcttcg tccaaagtca caacattatt ttcacttaaa ttagtcaatg ccatctctac
    24301 catacttaca gccccctcaa caatcttttt acgtgctgct attatagcct ctgcttgttg
    24361 acgttgtaac attgctgctg caatctctgg tgcataagaa agatgagtaa ttctcacttc
    24421 acacacttca attcctgcaa tgtcaaccct tgattgtaat tcatctttta atctatctgc
    24481 tatttcctga ctactaccac gaagagattt ttcatctcca tcttcagata catcatatgg
    24541 ataaagtctt gaaacattac gaatagtaga atcacactga atagataaga atgtattata
    24601 attatccaca ttaaatactg cctttgttgc atctataacc ttccaaatta caaccactcc
    24661 tattataatt ggatttccca ataaatcatt gactttctgc ttttcattat taagagtcat
    24721 tgtttttaaa gatacttttt tgccccttga attagttgat atatctacct tttcatcaga
    24781 tgaagatttc gatacattaa ttcttgatac tgctgggttt attgctgaac aaaatggatt
    24841 cacccaatag tacccttctt tttttatagt tccataatag ttaccaaaca aaactagaac
    24901 tatagcttct ttaggattaa tcatttttag cccaaaaaat aggataaaat ctactactat
    24961 aaatacaagt cccaaaatta acataagtga acctattcca gtaaatccac tatcaagttt
    25021 tactatccca aaaaccatga tacctattga aactattaaa aataataaaa tcattatcaa
    25081 agctgccact ccattgaata cttttgcgtc tttctcttga taagtttttt cttcttttaa
    25141 catatctatc actcccatct cagtatatta agttaagata tttctaattt aataataaca
    25201 cttttaacaa ttaaattgtt gaatttaaaa ttaatactat atgtttatat attttttata
    25261 catctttatg catacatgta tttagttatt ttaatgtatt aatattaatt tccaaataaa
    25321 tcatatgata tatgaatatg gtatttcaaa attaactttt tagttcatgg ggcactcttt
    25381 ttggtatgaa gtcaaatata tttttatcat ttaaacaatg aaaaagaaat ttatctatat
    25441 tttgaaaaat ccacttaaat tttttaatgt aagataaatt gtaagcttat tatcactaat
    25501 attattttat ataaattata ttagtgataa aataaactta tagaaatata acaaattttt
    25561 attaatttca taggaggaat ttatgttgaa agttataatt agtggctata atggaacaat
    25621 gggaagagtt ttagcagaat gtgtaaaaaa agataatgaa ttagagttgg tttgtggtgt
    25681 agctaggtct gctgatgaaa aaggtcatca tagtgataca aagctttaca aaacaatgac
    25741 tgatgtaact gaaaaaactg atgtaattat agacttttca catcatgaga ctttagagcc
    25801 aatattgtca tatgctctaa aaaataatac atctgtagta ttagctacta ctggatatga
    25861 cgaaaatgac ttaaataaaa tagaagaagc atcaaaaaac attcctatat tattatcatc
    25921 aaatacatct cttggagtaa atatattaat aaaattagtt aaggatgctt caaagatgtt
    25981 agaaggtttt gatattgaaa tcatagaaaa acatcacaat agaaaagaag acgcccctag
    26041 tggaactgct actatgatag ctaatgcaat tagagaagtc ttgcctgaaa gagaaaatac
    26101 acatggtaga tatggtagag attgtaaaag aaaaccaaat gaaattggaa tacactctat
    26161 aagaggtggc aatataatca gcgaccatga tgttatattt gcaggtgata atgaagttct
    26221 tacattaagc catcattctc aatctgatga agtatttgca aacggttcta tagttgcagc
    26281 taaattttta attggtaagc aatctgggct ttataaaatg tcagatatat taggataagt
    26341 tttgtacata ataaatctca atctatttag gtattcatat tcatcaattt gaatatctaa
    26401 ttgattgaga tttctattct aaaagtttat aaattttatg cttacctttt aaatataatc
    26461 atttttctta aagattatct tatttattgg tatatggaat tatattgttt attattttat
    26521 aatctcttca tcatataaac ctattaaata ttaccctatt tattggtata tggaattata
    26581 ttgtttatta ttttataatc ttttcatcat ataaacctat taaatattat cctaatttat
    26641 tgctatatga aatcatattg tttattattt tatagtcttt tcatcatata aacctattaa
    26701 atattatcct atttattggt atatgaaatc atattgttta ttattttata gtcttttcat
    26761 catataaacc tattaaatat tattctattt attggtatat gaaatcatat tgtttattat
    26821 tttataatct tttcatcata taaactattg tattctataa actgatacat atccagctgc
    26881 agaagtacct gtcgtgcctg aaccaaatgt aaaacttaaa gtaacagctg ctgctctagc
    26941 atcaactaaa aagcttaaac ttacacttac atctgatgaa gtaagtgtat ttactgcttt
    27001 tgcaataaag ttattttgag ctactccatt aaagttagta gtaattgaca taaatcctgc
    27061 aagtgaaagt gtggcacgta taccgaaaga tacattgtat atccctccaa ctgcaagatt
    27121 tatagtacta cttgttggaa aagttatatt agttccattt ataaatgatg aattaaatgt
    27181 cactaatgta ttattcacta aacttgaaga ggacactgta aattgtgcat tgtttgctgc
    27241 taacactcct gttgctcctg tcgctccggt tggacctgtt gctccatttg ctccggttgc
    27301 tccggtcgcc cctgttgctc ctgtcgctcc atttgctccg gttgggcctg ttggtcctat
    27361 cgctcctgct actccatttg ctcccgttgc tcctactgct ccggttgggc ctgttgctcc
    27421 atttgctccg gttgctcctg tcgctcctgt tgctcctgct actccatttg ctcccgttgg
    27481 acctgttgga cctattggac ctgctactcc attcgctccc gttgctccta ctgctcccgt
    27541 tgggcctgtt gctccatttg cccctgttgc tcctgtcgct cctgttggac ctactgctcc
    27601 atctgctccc gttgggcctg ttgctcctac tgctcccgtt ggacctattg ccccagctac
    27661 tcctgttgct cctgtcgctc ctgttggacc taccaaacca tttgctccgg ttgctcccgt
    27721 tgggcctgtt gggcctacca aaccatttgc tccggttgct ccggttgggc ctgttgggcc
    27781 tgttggacct accaaaccat ctggacctgt tgctcctact gctcctgttg gacctacctc
    27841 tccatctgct cctgttgctc ctgttgctcc tattgggcct gttgccccag ttgttcctgt
    27901 tgctccagtc gctcctatac ttcctgttgg acctgttgct cctgtatttc ctgttgggcc
    27961 tgtagcacct gttactccat ttgctccggt tgctcctgta tttcctgttg gtcctgttat
    28021 tccatttgct ccggttgctc ctgttgctcc agttagtccg gttggacctg ttgctcctgt
    28081 tgctcctgta ttccctgttg gacctgttat tccatttgct ccagtcgctc ctgtatttcc
    28141 tgttggtcct gtagaacctg ttattccatt tgctccggtt gctcctttgt ttcctgttgg
    28201 tcctgttatt ccatttgctc cggttgctcc tgtattccct gtcggacctg ttattccatt
    28261 cgctccagtc gctcctgtat ttcctgttgg tcctgtagaa cctgttgttc catttggtcc
    28321 agtcgctccc atatttcctg ttggacccgt tattccattt gctccagttg ctcctgtatt
    28381 tcctgttggt cctgttattc catttgctcc ggtcgctcca gttgcacctc taggccccgt
    28441 cggtcctgtt ggaccagtag cacctgttgg ccctgttgga cctacatctt gatataaact
    28501 tggacctgaa atatcactca tataattaat cctccttttt taaagttaga gtaatactct
    28561 ataaatattg agtattactc tatataatta tatgaatcga caatttctta tgtgataatg
    28621 tatctatttg cttatatcaa catatatgct ttgattctta aatttcatta cttttttgta
    28681 aaatctttta caattattag tactctttta caattattaa tacaattaaa aaactcattt
    28741 cccatcaatt atttaaaatt tttttattat gttataaaaa atagataatt aaattgcata
    28801 aaattaattt aatactactt taattacttt ttaaataaaa aaacctcaac acgattaata
    28861 aaaatcatat taaggcatcc acaaaattat ataattgctt atctatcttc atcagtaaca
    28921 ttgaatatat aaggaacatt tctataataa tcaccaaaat caaacccata tccaaccaca
    28981 aacttatctt ctatttcaaa tccacaataa tctggcacta aatcgacttt tcttctgctt
    29041 ggtttatcca aaagaacaca gcattttaaa ctagcaggat ttttagcttt tagatgtttt
    29101 aacacaaaat ccatagtaag tgcagaatca gtaatatcgt ctacaaccaa aacatcatat
    29161 cctgctatat cagtagtcac atctgaaact actttaacct ttccagtact ttcttcattg
    29221 tttccataac tagatgtagt cataaaatta actctaagtg gtaagtttat atttctaact
    29281 aagtctgcac aaaatataaa gctacctttt aatagagaaa ctaccataag atttttatct
    29341 ttataatctt tttctataat tgctcctaat tcccttaatt tatctttaat tttttcttct
    29401 gaatacaata cttcccattt ttttgtttct atatccataa aaagacctcc aattgtaata
    29461 atccttctta attttataag agattgctca ctttttcaat actcttattc aatattagtc
    29521 aatattattg agtttttatg ttcattcaac aattattaac aatgtatctt atattttaag
    29581 tagttcatat atccacacta aattacatac tatatatttt agacaaaaaa agaaaaaact
    29641 tgcactaaca agttttttct attatacata aatttataca taatcatatt ttattagagg
    29701 gtactaatta aatttattta tgctattttt gttatacctc ataatacaaa acatattata
    29761 aagtactagt caactttatt catatcattc attacattta agattatctc atctgttata
    29821 gacatacctt tgtcacttac tctacctaag ttttgtatac tttgctcaac acttgaacca
    29881 actataccat ttagtggtgg tacaaaacaa tcttgcttag ctattatagc actttgtata
    29941 gctgcagatg ctgcagaaga taattttaat gcacaaccag ctttcgcacc gtcacatatc
    30001 attccactta agtcagctat catattttct atagctcctt ctatttgctt ttcagtacca
    30061 ttcataagcc aagagatacc agcagttgct ccagtagaag cagcaacacc acatccacat
    30121 acagcagata atcttcctgt atagttctta atataaccag ttactagatg agatatagct
    30181 aaagcttttg ctagtctttc atcagattgt gggaattttt tattataagc aacgattgga
    30241 agaattgctg ttaatccatg atttccactt ccatttgaac tcataacagg catttttacc
    30301 cctgccattc ttgcatccga agctccagca gttaacatca ttgcataatt tataacatca
    30361 ttacctaaaa gaccttcttc tatagatttt ttaattccat atccgacacc aataccagtt
    30421 ttttgtttta atccatattc agccatctct tcattcattt ttactccatc tagtaaaaat
    30481 tctatatctt taaaatcaat ttcttctaca ttttttatta attcttgtat agttactgta
    30541 tccattaaac tttcagcttt ttctgccgca tcatttgaag ctgatacttt tggttcattg
    30601 tctaataata cttctccatc tttttctaag aacgtaaaat tatcatgttt agttcttatt
    30661 ctcactttag caatatgatt ttttcctttt aacacaactt ctataaatac tttctctctt
    30721 gtatcagcag gtgttattga tagtggagta ttatccatat aatcctcagc tattttaact
    30781 tcctcttttg ttagagtttc taacacactt aatccatttt cagaatgtcc acctactata
    30841 cctagagctg ctgcaatttt taatcctaat ctttcagtac ctggtatacc tacgcacata
    30901 ccatttttat aaatactagg actaactaac atacgatttt caactatttc ttctcctaat
    30961 aattcctttg cctttgcaca tgctagagct aaggctactg gttcagtaca ccctactgct
    31021 ggtttaactt ctgcctttaa catttccact aattcttttc ttatatctct cattatatat
    31081 aaacctccca attaagttta gatttagtac tcaaataatt aattaaataa tttcttatct
    31141 atatttatag caaaatacat gccatatttt ttatatcttt ttatattttt agtcttatat
    31201 tctataacca ttggtatgac tgcttctatt tatgtatttt ttttatttac atagagtatt
    31261 tatattcttt aaaatggaga aacatatgac aatttcgcaa aaagacaaaa aaaagtctca
    31321 aaatgagatt ctatatcatt ttgagacggc tcaaatgtat tattttttaa cttataaaaa
    31381 ccatttttta attcataaaa aaagattctg caaaacactt atagtataag tttaaagctt
    31441 tctttaaagt tatgaattta gaaaaattta aattttatat agctttttat tatattacta
    31501 tgaaattata ttgtattttt caatttttct atataatgta gctcttgata ttccaagtgc
    31561 tttagctgtt aattctttat cttttttata atctttgtac ttatttaaag cttttattat
    31621 ctcaatcttc tctagttcat caagtggaat tatcctatcg cttttaactt ctaactcttc
    31681 tacagaggta ttcttaattt tttgaggaat tacattcatt gtgattattg aagaagaaga
    31741 catattcaca ctatattcaa taatattctg caactctctt atattccctg gccacttata
    31801 ttccatcata gcatcaacaa ccctgctatc tatgtcaata acatttttat ctaatttttt
    31861 tgaatactct ttaaccatat gcttgattat aagtgggata tcttcttttc tctctcttaa
    31921 acttggtaat tttataggta taacatttaa tctataatat aaatcttctc taaaagtccc
    31981 attttgaacc attttttcta aatctttatt tgtagcagca ataattctta catctattga
    32041 aacatttgat ttacttccaa ttttattgag ttccttttct tgtaatactc gcaataactt
    32101 tgactgtaaa tgcaaactca tatctcctat ttcatctaaa aatatacttc ccttatgagc
    32161 tacttcaaac attccaagct ttccaccttt tttagctcca gtaaaagctc cctcttcata
    32221 tccaaataat tcactctcta ataaattgtc tggtatagca gcacagttta cagctataaa
    32281 aggattatct actctattgc tatggttgtg tatggctctt gcaaatagct ccttacccgt
    32341 tccactttcc ccagtaataa gtactgttga tgttgattta gaagctatta gggcttcctt
    32401 tttagaattt cttatagcta cactttctcc aattatgttt tccaatttta ttctataatc
    32461 tttattcatc ttattgtagt tttttatagc atctctttta tctataaaat caataacata
    32521 tccctttaac tcatttttta atactatttt attgatgttg tatattccct tcgaatcata
    32581 tccgtatctt ttataaaaaa aactacagga tttgtccttc ttaaagttgt ttatagaagg
    32641 tttctttata aaatctaatt cgacaaatat gtttttgcca gttatactat cttttatatt
    32701 gaaaagctct ttaaattttg aattatattt atcaattttt ccatccacat caatagacac
    32761 aacagctttg tccatattgt ttaatagtgt ctctaatttt ttcttttcta attcaagctc
    32821 ataagtttta atttgtgctt ttaatttatt agatattagg tctgccattt tgcctaaaaa
    32881 gtttataaga ccacttttat tattttctat aatttgtgct tgttcttttg taaaggctat
    32941 aaggcctata acaccatgag aatcgccatc acatataata ggacaacaca cctctgcaaa
    33001 ctctacacaa gccccacttt tatagcattc cctacatata tcactactct ttgggtcatc
    33061 aattagtatt gtaatctgtt ctgtaagtga ttttttaaaa gccgtatatt tatctacttt
    33121 ctcacctatt ttgtctatat atactcctgt acctgcaatc ctaaataact ccttatttac
    33181 tatagttaca tctatattga gtactgatac tatagcttca gctatatttt gaatatcaga
    33241 ttttatatat tctaaaccta tcataatcat cattccttat ttttctttaa cataagtatt
    33301 tgtcttattt ctttaagaat caagtatatc ttatttaaag taaattggat tgacatcagg
    33361 attacaaaga gcataacaat tattattaac tcataattta tttccatcta aacatctcct
    33421 tataacaaaa gactaggctt aacctagtct tttatattat atccatattt atattttaac
    33481 tcatttatct tctttttccc acataaatat attttaattt atttgactct ttaatcatac
    33541 catcaaagtt taatcttgaa aatatattaa atacttcatg tggatagtga atctttgcaa
    33601 cagaaccaca aaatttatgt ttaagatagt ttaacttaac ttttaaattc tttaaattta
    33661 aatcctctaa tataacctct ccattgccct ttaacactct ttgtatttca gatgaagctt
    33721 tttcacaatc tgtaataaca ttaaattggt caactattat tatcttgttg aactgattat
    33781 catcaaatgg tagaaactct aacttacctt taactacatt gcaattttca ttatacacta
    33841 gagaataaca gacatcttca taattactat tctccagtat agttacagaa gttccaagac
    33901 ctctcaatct cttgcctagg tttcctatat gaccaacaag caatatttta tcacctacat
    33961 tatactcaat tgtatccaaa aaacattcta attcatcaga tgtatattta gctttaactg
    34021 gctgtttatt tatctgacat aatatgcttt tcacaatgct tcacctcttc atttcctcaa
    34081 ttataaatta tgtacagatt acattataat aaatggcgag caactattca agagatattt
    34141 cattagtatt tttttaaaca atctttatac attttaatat gatattatac caaatttatt
    34201 aggattttat tcataaaaat cagtctattt ttcatgttta ttcattgtta ttttcatcca
    34261 atttttttat tttcgatatg gatacttcat atgccatttt tttgaccaca ttattttcat
    34321 ctattttttt ctcatattct ctactttgaa ccctacccca aagctgtatt ctatcaccaa
    34381 cctttagatt ctttgcaaat ttagcatttc taccccaaac tattgatggt atataatcag
    34441 atttattata aggtctgttt atagcaacta ataaatcggt tatttccctt cctaaaggtg
    34501 tcttcctata cacaggttcc ttacatacat atccatctaa aaatatacta ttagggtctt
    34561 tattttcctc gtctatgctt tttatttctc tgacaaacac tgtaagcact aatctatttt
    34621 tattatttaa tgttttgtta tatgatctta attggcctac gacattgact aaattttgtt
    34681 tatcaaggtc aatatcttgg agaagtcttt ctgatacagt cattggaagt atatcaaaag
    34741 aatcgcttag tctgtttatt tttattttca tattataaaa tttttctcca aatatctcat
    34801 ggctaaattc tagcttattg tctaactccc ctcttaagtt tactacgtta ttctcatctt
    34861 taacagctat catattttat ccctccacaa ttgttaattc ttatatttct ggtatcagct
    34921 tatataataa atatatttat aagggatgta ttttattaca aatctttgat tgctgtttaa
    34981 aagtttttat ttagttataa attt
//
LOCUS       DAELJO010000017        34763 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELJO010000017 DAELJO010000000
VERSION     DAELJO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.017"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            139..615
                     /gene="purE"
                     /locus_tag="KPB88_000770"
     CDS             139..615
                     /gene="purE"
                     /locus_tag="KPB88_000770"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG0053072.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            621..1319
                     /locus_tag="KPB88_000771"
     CDS             621..1319
                     /locus_tag="KPB88_000771"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0053073.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            1331..2698
                     /gene="purF"
                     /locus_tag="KPB88_000772"
     CDS             1331..2698
                     /gene="purF"
                     /locus_tag="KPB88_000772"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG0053074.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            2692..3768
                     /locus_tag="KPB88_000773"
     CDS             2692..3768
                     /locus_tag="KPB88_000773"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG0053075.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVSGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            3762..4355
                     /locus_tag="KPB88_000774"
     CDS             3762..4355
                     /locus_tag="KPB88_000774"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG0053076.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            4348..5880
                     /gene="purH"
                     /locus_tag="KPB88_000775"
     CDS             4348..5880
                     /gene="purH"
                     /locus_tag="KPB88_000775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG0053077.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNKIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            5891..7141
                     /gene="purD"
                     /locus_tag="KPB88_000776"
     CDS             5891..7141
                     /gene="purD"
                     /locus_tag="KPB88_000776"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG0053078.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            7166..10972
                     /locus_tag="KPB88_000777"
     CDS             7166..10972
                     /locus_tag="KPB88_000777"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG0053079.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESALTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            11178..12050
                     /gene="rfbD"
                     /locus_tag="KPB88_000778"
     CDS             11178..12050
                     /gene="rfbD"
                     /locus_tag="KPB88_000778"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG0053080.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            12159..13028
                     /gene="rfbA"
                     /locus_tag="KPB88_000779"
     CDS             12159..13028
                     /gene="rfbA"
                     /locus_tag="KPB88_000779"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG0053081.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            13077..13634
                     /gene="rfbC"
                     /locus_tag="KPB88_000780"
     CDS             13077..13634
                     /gene="rfbC"
                     /locus_tag="KPB88_000780"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG0053082.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            13672..14655
                     /gene="rfbB"
                     /locus_tag="KPB88_000781"
     CDS             13672..14655
                     /gene="rfbB"
                     /locus_tag="KPB88_000781"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG0053083.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            14685..15353
                     /locus_tag="KPB88_000782"
     CDS             14685..15353
                     /locus_tag="KPB88_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0053084.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            15362..15790
                     /locus_tag="KPB88_000783"
     CDS             15362..15790
                     /locus_tag="KPB88_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG0053085.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            15814..16128
                     /locus_tag="KPB88_000784"
     CDS             15814..16128
                     /locus_tag="KPB88_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0053086.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            16280..16555
                     /gene="flgM"
                     /locus_tag="KPB88_000785"
     CDS             16280..16555
                     /gene="flgM"
                     /locus_tag="KPB88_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG0053087.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            16560..16976
                     /locus_tag="KPB88_000786"
     CDS             16560..16976
                     /locus_tag="KPB88_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG0053088.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            16994..18304
                     /gene="flgK"
                     /locus_tag="KPB88_000787"
     CDS             16994..18304
                     /gene="flgK"
                     /locus_tag="KPB88_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG0053089.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            18323..19255
                     /gene="flgL"
                     /locus_tag="KPB88_000788"
     CDS             18323..19255
                     /gene="flgL"
                     /locus_tag="KPB88_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG0053090.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            19271..19663
                     /locus_tag="KPB88_000789"
     CDS             19271..19663
                     /locus_tag="KPB88_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG0053091.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            19657..19869
                     /gene="csrA"
                     /locus_tag="KPB88_000790"
     CDS             19657..19869
                     /gene="csrA"
                     /locus_tag="KPB88_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG0053092.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            19891..20286
                     /gene="fliS"
                     /locus_tag="KPB88_000791"
     CDS             19891..20286
                     /gene="fliS"
                     /locus_tag="KPB88_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0053093.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            20286..20642
                     /gene="fliS"
                     /locus_tag="KPB88_000792"
     CDS             20286..20642
                     /gene="fliS"
                     /locus_tag="KPB88_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0053094.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            20663..22186
                     /gene="fliD"
                     /locus_tag="KPB88_000793"
     CDS             20663..22186
                     /gene="fliD"
                     /locus_tag="KPB88_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG0053095.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            22189..22524
                     /gene="fliT"
                     /locus_tag="KPB88_000794"
     CDS             22189..22524
                     /gene="fliT"
                     /locus_tag="KPB88_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG0053096.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            22626..23498
                     /locus_tag="KPB88_000795"
     CDS             22626..23498
                     /locus_tag="KPB88_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG0053097.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            23591..25732
                     /locus_tag="KPB88_000796"
     CDS             23591..25732
                     /locus_tag="KPB88_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0053098.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            25756..27633
                     /locus_tag="KPB88_000797"
     CDS             25756..27633
                     /locus_tag="KPB88_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0053099.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            27626..29740
                     /locus_tag="KPB88_000798"
     CDS             27626..29740
                     /locus_tag="KPB88_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0053100.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            29749..30744
                     /locus_tag="KPB88_000799"
     CDS             29749..30744
                     /locus_tag="KPB88_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG0053101.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            30836..32068
                     /locus_tag="KPB88_000800"
     CDS             30836..32068
                     /locus_tag="KPB88_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG0053102.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKKASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQVFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            32088..33032
                     /locus_tag="KPB88_000801"
     CDS             32088..33032
                     /locus_tag="KPB88_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG0053103.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCKTLNCEEIELKEPKEKYWI"
     gene            33126..33533
                     /locus_tag="KPB88_000802"
     CDS             33126..33533
                     /locus_tag="KPB88_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG0053104.1"
                     /translation="MDKYNLIKFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGMGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            33620..34711
                     /locus_tag="KPB88_000803"
     CDS             33620..34711
                     /locus_tag="KPB88_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG0053105.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEKKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YHNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIERSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLNKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMKDEEVSYIIKIINEY"
BASE COUNT        14307 a         3449 c         6391 g        10616 t
ORIGIN      
        1 gaaatatttt ttttaaaaaa tttcaaaaaa gagttgataa tatatgtata ttaatataaa
       61 atttaaggtg tataattcgt ataataatac gaatgatttg tagtgtaaaa taacttataa
      121 acggagaggg gaagtctaat gaaagtagca gtagttatgg gttcaaaatc agattatcca
      181 aaattagaag aagggataaa actgcttgaa aaatatggaa tagaagttgt agcaagagca
      241 ttatcagctc atagaacacc agaacaactt tcaatattcc taaaagaaat agaagatgat
      301 actgatgtaa taatagcagc agcaggaaaa gcagcacact taccaggtgt aatagcttca
      361 caaacattaa ttcctgtaat aggattacct attaaatcat caactatgga tggactagac
      421 tcacttttat caatagttca aatgccaaaa ggaatcccag ttgcaacagt tacaatagac
      481 ttaggtttaa acgcagcttt attggcatta caaataatga ctttaaaata cccaaaatta
      541 aaagaggatt tgaaatcata tagagaagag atggcacaaa aggtgttaga agacgacaag
      601 aatttaaggg ggtagcagaa atgttattat atgaaggaaa agcaaaacaa gtatattcta
      661 cagataatga aaatgaatat gttgtttatt ataaagatga tgcaacagca tttaatggag
      721 aaaaaaaagc agaaatatct tcaaaaggta tactaaataa taagatatca acaataatct
      781 ttgaaatgtt aaaagaaaat aatataaata ctcactttat aaaaagttta tcagatagag
      841 aaatgttagt taaaaaagta gagattttac cattagaagt aatagtaaga aatattgctg
      901 ctggttctat ctgtaaaaga gttggtcttg aagaaggtgt agttttcgat gagccaattt
      961 ttgaaataag ctacaaaaat gatgcttatg gtgacccaat gctaaatgat gattatgcag
     1021 tggcaatgaa gttagcaact agagaagagt taaaattctt aagagaagaa actttaaaaa
     1081 taaatgagtt actaaaagca tttttcttaa aattaaacct aaagttagtt gactttaaaa
     1141 tagaatttgg taaggattct gaaggaaata taatattggc agatgaagta tcaccagaca
     1201 cttgtagact ttgggatgta aatacaaatg aaaagttaga taaggacaga tttagaaaag
     1261 accttggaga ccttgtagaa ggttatacgg aagtactttc aagaatgaat aataaatagg
     1321 aggcaaatgt atgtgtggag ttttagggat ttactctaat aaagatgtaa ctaaagaact
     1381 atattattct ttatattcta tgcaacacag ggggcaagaa agctgtggtt tagctctttt
     1441 agatgatgga gaaattaagt acaaaaaaga tatgggatta gttggagatg tttttaagga
     1501 aaatgaatta tctaagttaa aaggaaatat agggattgga catgttagat attcaacagc
     1561 tggaggaagt catgtttcta actgtcaacc tttagttgga agttgtagaa aaagacaatt
     1621 agccatagct cataatggaa atctagttaa tgcaaattat ttaaaagata tgctagaaga
     1681 agatggatat atgttccaaa ctaattcaga tacagaagtg attttatata tacttgctag
     1741 atattacaaa ggggatatag tagaaagttt aaaagtgaca atggattata taaaaggtgc
     1801 atatgctctt gtaataatga gtcaagaaga attggttgca gttagagacc cacatggatt
     1861 cagacctctt gtacttggta aaaagggtga tgaatatata tttgcatcag aaaattgtgc
     1921 aatagatata cttggcggag aagttataag agatgtagag ccaggagaga taatagtagt
     1981 caaagatgga gaactaaaat catattttta ttcagaaaat tataaaccag ttaagaaaag
     2041 ttgtatattt gaacacatat actttgcaag aaatgatgca actatagaca atgtaaatgc
     2101 atatgaattt agaattaaat gtggtgagag attagctcag aatgaaactg taaaggcaga
     2161 tatggtagtt ccagttccag attcaggttg gccaggagct attggttatg caaatgctag
     2221 tggattaaaa ataagtgaag gcttagttaa aaatagatat gtaggaagaa catttataaa
     2281 acctacacaa gaagaaagag aaatagcagt aaaaataaaa ttaaatcctc tttcaacaat
     2341 aataaaagga aaatctataa ttttagtaga cgactcaatt gtaagaggaa ctacatctaa
     2401 acaattagta aaatctttaa gagaagcagg agcaaaagaa attcatctta gaataacttc
     2461 tcctccagta gcttactctt gttactatgg aatagatact ccaaatcgct ctaaattaat
     2521 tgcatcaagt aacaacgtag aagaaatgag agaatatatt ggatgtgata gtttaaaatt
     2581 cctagatata gaaggaatgt tagatgcaac agaacataag tctactttct gtaaagcttg
     2641 ttttgatgga gaatacccag ttaagaaaat tgataaggag gaactacttt catgttaaca
     2701 tataaagaat caggtgtaga tatagatgaa ggaaatagag cagtagacct tataaaaggt
     2761 aaaataaaag gaacttatga tggaaatgta gttggagatt taggtaattt cagtggatta
     2821 tacagtttaa aagattttgt aggtatgaaa gaaccagttt tgcttgcatc tacagatggt
     2881 gttgggacta agttaaaaat agctcagatg atggacaagc atgatactgt tggaatagat
     2941 ttagttgcaa tgtgcgtgaa tgatttaatt tgtcaaggag caaaaccatt attcttcctt
     3001 gactatatag ctcttgggaa attagtgcct gagcatatcg aaaaaatagt tagtggaatt
     3061 gcagatggtt gtaaaatgtc tggttgtgca ctaattggtg gagaaactgc tgaaatgcct
     3121 ggaatgtatg gagaagatga ctatgattta gcaggatttt ctgtaggaat agcagacaaa
     3181 gaaaagattg tttctggaaa caatgttaaa tcaggagatg tattggttgg tatatcttca
     3241 agtggagtac acagcaatgg attttctttt ataagaaaga tatttttaga aacttacaat
     3301 tataaaatgg aacaatatgt tgaagaacta ggaatgacag ttggagaagc acttttaaca
     3361 ccaactaaaa tatatgttaa attagcttta gatgtattag ctaaacatga cataaaagct
     3421 atagctcaca taactggtgg agggcttata gaaaatataa caagagttat acctaaaggg
     3481 ttaggactag atatcaataa aaaatcttgg gagaagcctc ctatatttaa aatgatagaa
     3541 ggttttaatg ctgttgatga gagagaactt cataagagtt ttaacatggg tattgggcta
     3601 gttttaatag tagataaaga aaatgctgat gatgttgtaa actttataaa taatagagaa
     3661 aatgacaatg ctgcttatgt agataaaaaa tatagtgaat tattagaaga taaagcgtat
     3721 ataattggtg aagtagtaga tagtcatgaa ggtgtagaat tatgctaaat ataggagttt
     3781 taatatctgg tggaggaaca aatttacaag cagttataga tggtactgag tctggagaaa
     3841 taaaaggtca ggttaaagta gttatctcaa gtaagcaagg tgcttatggt ctagagagag
     3901 ctaaaaatca taacataaaa gcaatttgtg aaactgatga agacaagata atagaaattc
     3961 ttaaggaaaa taaaatagat ttagtagttt tggctggtta tttaaaaata atcagcccaa
     4021 agctggttaa tgagtttaga aacaaaatga taaacataca tccatcatta attccttcat
     4081 tttgtggagc tggattctat ggagaaaaag ttcatcaagg tgttatagat tacggagcta
     4141 aagtaactgg tgctacagtt cattttgttg atgaaggtgc tgatactgga ccaatcataa
     4201 tgcaagatgt agttaaggta aatcaagatg atgatgcaaa gacacttgca aaaagagtgt
     4261 tagaagttga acataggatt ttaaaagaaa gtataagtct tttttgtgaa aataaattaa
     4321 aacttcaagg taggagagtg tttataaatg agtaagagag ctttaataag tgtaactgac
     4381 aagactggtg tagtggaatt tgctaaagag ttaaataaat tagattatga aattatatct
     4441 acaggaaata catttaaaac attaaaagaa aatggtgtta atgtaatgca agttgaagat
     4501 gttacaaatt ttccagaaat attagatgga agagtaaaaa cattaaatcc atatatacat
     4561 ggtggtatac tttataaaag agataaagaa tctcatgtag aaactgtaaa tgaacacaaa
     4621 atacattcaa tagatttagt agctgtaaat ctatatgatt ttgaaggaac tttaaaagct
     4681 ggaaaaagtc atgatgaaat aattgaaaat atagatattg gtggaccatc tatgatacgt
     4741 tctgctgcca aaaactataa ggatgtaata gtagtagtag atataaaaga ttatgattct
     4801 ataatagaaa aattaaaaac agatacaatg acattagaag acagaaaaaa attatcatat
     4861 aaagctttct caactacagg aagatatgat gcgttaatat ctagttattt tgctggagaa
     4921 gttggagata cgtatcctga catattaaat ttaactttcc aaaaagaaca aactttaaga
     4981 tatggagaaa atccacatca aaatggtttc ttatattcac agtcaaatgc aaaaaatcca
     5041 atcttaaatt atgagcaatt aggcggtaaa gaactttctt ttaacaactt aaatgattta
     5101 catggatgtt tagaagtaat gagagaattt aaagatagtg aagaagtagt ttcagtagct
     5161 ataaaacatg ctaattcatg tggggtaggt ttaggaaaag atgcttttga agcatataca
     5221 aaatgttatg aagcggataa ggtttcaata tttggaggaa tagtaggaat aacttctact
     5281 atagataaag ctacagcaga gaaattaaat aaaatattct tagaaatagt agtagcatat
     5341 gattttgaac cagaagcttt agagatactt aaacaaaaga aaaatctaag aatattaaaa
     5401 ttagctaaaa tagaaaatag tttacaacca tatgaaatga aatatttaga tggaaaatta
     5461 ttgatacaag atagaaacaa tatattagca gaaaaaagtg aaaatgtaac aaaggaaaaa
     5521 ccaacagatg cacaacttaa ggacatggaa tttggtatga gagtagtaaa aaatatgaaa
     5581 tcaaatgcaa ttgcaatagt aaaaaatgga caaactctag ctttaggttg tggacaaaca
     5641 tcaagaattt gggcattaaa aaatgctctt gaaaataaca aagataaaga ttttacagga
     5701 gctgttttag cgtcagacgc atttttccca tttgatgatt gtgtaacatt agctcatgaa
     5761 tatggaatat cagcagtagt tcaaccaggt ggctctataa aagataaaga ttcaatagaa
     5821 gcttgtgaca agtatgatat ggttatggta tttacaggaa taagacactt caaacactaa
     5881 ggaggatatt atgaagatat tggttgtagg cggtggcgga agagagcatg ctatatgctg
     5941 gaaactaagc aaagaaaaaa atgtagaaaa aatttattgt gcaccaggta atgcaggaat
     6001 tgcaaacgta gcagaatgtg taaatatagg tgatactaat atagaagaac ttttaaaatt
     6061 tgccaaagaa aatgaaatag gtctgacaat agtaggacca gaagtaccac tagttatggg
     6121 aatagtagat gagtttgaaa aagaaggatt aagagtattt ggacctaata aaaaatgtgc
     6181 tcaacttgaa ggaagtaaag ctttttcaaa agaatttatg ataaaacata acatacctac
     6241 tgcaaaatat aaagaatata caaacttaga agaagctata tcagaaatag atagttttgg
     6301 atatccagta gtaataaaag cggacggatt agctgctgga aaaggtgttg taatacctga
     6361 aaatagagaa gatgctatag caactttaaa agaaatgatg tcagataaga agtttggagc
     6421 tgctggagat aaaatagtaa ttgaagagtt tttaaaagga atagaaactt caattctagc
     6481 atttgtagac aatgatacaa tagttcctat ggcaagtgca aaagaccata aaaaagtaaa
     6541 caactatgag caaggtccaa acactggggg aatgggaact ttttcaccta gtgaaatata
     6601 tacagaagaa ttagcaaata aagtaaaaga gacagtatta gaaaaaactt tagaaggctt
     6661 caaaaaagat ggacttaatt ttaaaggtat tttatttgtt ggtcttatga taactgaaga
     6721 tggagaaaaa gtacttgaat ataatgtaag atttggagac cctgagacac aatctgtttt
     6781 atttagatta gaaacagatt tacatgagat aatggaagct atactagata ataagttaaa
     6841 agatatagaa ataaactata gtgatgatga agctgtatgt gttatgctta catcaggagg
     6901 gtatccagac agttatgaaa aagggaaaat tataactggt cttgaaaact tggatgatga
     6961 tatagtagta ttccatagtg gcactaagat gtttgatggt aatttggtta ccaatggagg
     7021 aagagtaatt ggtataactg caaagtcaac tacagttaag gatgcagcag aaaaagttta
     7081 tgaaaacata aagaagataa actttgaagg aatgcactat agaacagaca taggaagata
     7141 atttttctaa aatggaggat aaggtatgtt gaatactgaa aataaagact caatggttag
     7201 aagagtattg gttgaaaaaa gagaaggttt tgaccttgag gctaaagctt taaaaaaaga
     7261 cttagtagaa agtttacaca tagataatat agaaaattta agaatattaa atagatatga
     7321 tgtagaagga atatcagaag aagtttatga aaatgcagca aagacaatat tctctgaacc
     7381 aaatttagat gtagtatatt atgaagaaat accaaaatta aatgatgaaa gagtatttgc
     7441 aatagaattt cttcctggtc aatacgacca aagaggtgat tgggcagctc aatgtgtaca
     7501 aattgtaaat caagggataa gacctgctat taatacagca aaagtttata tattatcagg
     7561 caaaataact gatgaagagt tttctaaaat aaaagattac tgtataaatc cagtagatag
     7621 tagagaagca tctttagaga aaccagaaac gttaaaaatg gaaactgaaa taccaacaac
     7681 agttgaagta ttagatggat ttatagattt agatgaaaat ggattaagaa catttgtaag
     7741 tgaaaaagga ttagcaatga ctttaggaga cttacaacac gttcaaaaat actttaaaga
     7801 tacagaaaag agaaatccaa ctataacaga aataaaagta ttagatactt attggtctga
     7861 tcattgtaga catactactt ttatgactga aatagaaaat gtaaagatag aagatggtaa
     7921 gtttaatgat atagtaaaag aagcgtatca aatgtatctt aactcaagag acaatgtata
     7981 tgtgaacaga cataaagata tatgtttaat ggatatagcc actgttgcag taaaagaatt
     8041 aaagaaaaat gggaaattaa atgacctaga tgaaagtgaa gaaataaatg catgtagtat
     8101 aaacgtagat gttgaagttg atggtaagat ggaaaaatat ctagtaatgt ttaaaaatga
     8161 aactcacaac catccaactg aaatagaacc atttggtgga gcagctactt gtttaggtgg
     8221 agctataaga gacccattat caggaagaag ttatgtttat caagcaatgc gtgttacagg
     8281 aagtgctgac ccaagaacga ctttagaaga tactttacct ggaaaactta tgcaaagaaa
     8341 aataactaca gaagctgctc atggatatag ttcatatggt aaccaaatag gtcttacaac
     8401 tgggcaagtt gcagaagtat atgatgaaaa ctttgttgca aagagaatgg aaataggtgc
     8461 agttatagca gcagcaccaa aagagaatgt agtaagagaa agacctgaag caggagatgt
     8521 aatcgttctt cttggaggaa aaacaggaag agatggctgt ggaggagcaa ctggttcatc
     8581 taaagaacat agtgaagagt caatcttaac ttgtagtgca gaagttcaaa aaggagatgc
     8641 tcctaatgaa agaaaaatac aaagattctt tagaaataaa gaagttgctc aaatgataaa
     8701 aagatgtaat gattttggag caggtggagt ttgtgttgct ataggagaga ttgctgatag
     8761 cttagatata aacttagact tagttcctaa aaaatatgat ggacttgatg gaactgagct
     8821 tgcaatttca gagtcacaag agcgtatggc agtagccata aaaaaagaaa ataaagataa
     8881 atttatacaa ttagcagtag aagaaaactt agaagctacg catgtagcta cagtaacaga
     8941 tacaggatat ttaagaatgt tctggaatgg aaaagcaatt gtagatataa atagagagtt
     9001 tttagataca aacggagtaa aacaaactac tgatgttcat gttactaaag tagatgaaga
     9061 aaatacattc tttagctcta atgaaatagt aaaagatgtt aagtgtgcaa gtatgaaaga
     9121 caagtttaca aaagtactat ctgaccttaa tgtttgttct caaaaaggat tagtagagat
     9181 gtttgataat acaattggtg gtaatacagt acttatgcca tttggaggaa agtatcaagc
     9241 gactccaaca caaggtatgg ttgctaagat accagtactt ggaggagaaa ctaatacttc
     9301 tacaataatg acttatggat acaatccaaa ggtaggaaaa tggagcccat tccatggtgc
     9361 cttgtatgca gtagtagaat ctgtgtgtaa attggttgct attggaggaa attatagtac
     9421 aacaagactt acattccaag agtattttga aaaacttggt aacaatccag aaaaatgggg
     9481 taaaccattc tcagcacttt taggagcatt ttatgcacaa agtaaatttg agatacctgc
     9541 tataggtgga aaagacagta tgtctggtac atttaaagat attgaagttc caccaacatt
     9601 agtttcattt gctgttgaca cagtagatgc gaagaaagta gtatctccag aatttaaaaa
     9661 agcagactca aaagtagtaa tgttatgtgt aaacaaggca gaaaatgatg ttgtagattt
     9721 tgaggaatta aagagaaact tagataaagt aagagaactt atacatggaa ataaagtttt
     9781 atcaacatat gcattaggat ttgcaggtgt aggtgaagca ataagtaaaa tggcttttgg
     9841 taataaaata ggatttaagt ttagtgaaga ggctgaaaaa gcatttacag atgataaatt
     9901 atttgaggct agttatggaa atatagtact tgaattagca aatgatgatt taagtatgtt
     9961 agaaggatac aattatgttg tgcttggaag tacagttaaa gaagctagta tatttatcaa
    10021 aggtgaagaa cttgcattag atgaattata taaagcacat tgtagtactt tagagcctat
    10081 attcccaact aaaacagaag aagtaaaatc aaagatagaa actataagtt atatatcaca
    10141 aggagaagct aaaaaatcat cattaagtat agctactcca agagtattta taccagcatt
    10201 ccctggaaca aactgtgaat atgactctgc aagagcattt gaaagagcag gagctaatgc
    10261 aagtataaga gtatttaaga atttaactta taaagacata gaagattcta tagatacaat
    10321 agtaaacgaa ataaaatctt ctcaaataat aatgttacca ggtggattta gtgctggtga
    10381 tgagccagat ggttctggta aatttatagc tactgtattt agaaatccta gagtgcaaga
    10441 agctataaat gaatttttaa ctcaaaaaga tggactaatg ttaggtatct gtaatggatt
    10501 ccaagtatta attaaattag ggttagttcc atatggagaa ataagagttc caagtgaaag
    10561 tgctctaaca cttacttaca ataatattgg tcgccatcaa gctaagattg ctagaactag
    10621 aatttcatct aataaatctc cttggttagc tcaaactaat gttggagata ttcataatat
    10681 agcaatttct catggagaag gtaagtttgt tgccagtgaa gatgttatga gagaattaat
    10741 tgctaatgga caagtagcaa ctcaatatgt tgactttaat aatgaagcta cttatgatat
    10801 tgagtttaat ccaaatggtt cattctatgc tgttgaaggt ataactagtg ctgatggtag
    10861 agtgtttggt aaaatgggac attctgaaag aattggtgag gaagtatata agaatattat
    10921 aggtgaaaaa gaccaaaaga tatttgaatc tggagttaaa tattttagat aaagctttga
    10981 tgtttaattg atagctagaa tatataattt agatatttag atttaaagag atgtaataat
    11041 atgatgataa tcatttaata ttacatctct tttttattta tctaagaaat aataagttca
    11101 acctaagtta aaatacaagc tgtgttataa ttaattatta agtaaaattg aatatttatt
    11161 ggagggcaac ataattaatg aaaatattaa taacagggtc taatggtcaa ttaggaaaag
    11221 aattggttaa ccaattagaa gcaatcaatc aaagtataaa ccaaccaaaa tatgttatat
    11281 tagctacaac aagaagtgat ttggacatat ccaatcagac taatgtagat aattttattt
    11341 tgcataataa acctgatgta gttgtaaact gtgcagcata tacaaaggtt gatgcatgtg
    11401 aagataatat cgaaattgca tacaaaataa atgctttagg agtgagaaat ctagcaatag
    11461 catcagaaaa agttaatgct aagcttattc acatatcaac tgactatgta ttcaatggtt
    11521 tttctaaata tccatataga gaagacaata aaactgaacc caattctgta tatggaaaaa
    11581 gtaaacttat gggagaaaaa tttgttgaac aattctcaca taagtacttt atattgagaa
    11641 cagcatggtt atatggtaat gggaataatt ttgtaaagac catgataaaa ctttctttag
    11701 aaaataaaga ggttaatgtg gttaatgacc agtttggaac tccaacaagt acagtagact
    11761 tagcaaaaat tataataaga atcatggaaa cagaacatta tggagtttat catgctacat
    11821 gtgaaggtga gtgtagttgg tatgattttg ccaaaaagat atttaaacta aaaaatatag
    11881 atataaaagt aaatccaatt aagagtaacg aatttaaatg taaggctcca agaccactat
    11941 attctgtatt ggataatttt atgttgaagt taattggttt aaattctttt agaaagtggg
    12001 aagaatccat tgaagaatat ttagaaagat atgatataaa tgaaaagtaa atgtattatt
    12061 ttgagaaaag aaaataagat attataaaaa atataaatat ttcttttatt tatccgataa
    12121 tcataatatg acaaaaacaa atttaaggat ggaaaaagat gaaaggaatt attttagcag
    12181 gagggtctgg tacaagacta tatcctataa caaaatgtat atcaaaacaa acacttccaa
    12241 tatacgataa accaatgata tattacccta tgtcggttct tatgttggca gggatacaag
    12301 atatattaat aatatctaca ccaagagata taaaagcatt tgaagaatta tttggagatg
    12361 gaagttcact agggcttaac ataacatatg caattcagga gaaagcaaat ggaattgcag
    12421 aagcgtttat aataggagaa gaatttatag gaaatgacag agtgtctctt gtattagggg
    12481 ataatatatt ttatggatat ggatttagtg aaaggttgga gaatgcagtt aaaagaaaag
    12541 gagcaactat atttgggtat catgtaagta atccaaattc gtttggagtt gtagaatttg
    12601 ataaggattt taatgtattg tcaatagaag aaaaacctac agttcctaaa tctaattatg
    12661 ctataccagg attatatttt tatgataatg atgttgtaga gatagcaaag ctagttgaac
    12721 cgtcagatag aggtgaatta gaaataacct ctataaacag tgaatatcta aaacgaggaa
    12781 aactaaaagt ggaacttcta ggaagaggta tggcttggct tgatactggt acacataaag
    12841 gtctattgga tgcttctaat tatgttgaag tggttcaaac aagacaggga ctgtatatag
    12901 catgtttaga agagattgca tatacaaaag gatatataag tagagaacaa ttaatgaaac
    12961 tagccaagcc actgataaaa actgaatatg gtcaatatct attaaaattg gctagtgaag
    13021 atatgtaata tggtagataa aatttattat aaattttatt aagggagttt attataatgg
    13081 gaaaaatcaa tgttataaag actaaaatag aaggattata tatagtcgaa ccaaatgtat
    13141 tttatgatga aagaggatat tttatggaaa gctataataa aaaggatttt atagagcagg
    13201 gtcttgatat gtgttttatt caagataatg agtctagctc aagaaaaggc gttttaagag
    13261 gaatgcattt tcaggttaaa tatcctcaag gaaaattagt cagagttaca gagggagaag
    13321 ttttcgatgt tgcagttgat ttaaggaaag attcacaaac atttgggaaa tgggaaggtg
    13381 taatattgtc tgataagaat aaaaaacaat tttatattcc agaagggttt gcacatggat
    13441 ttttagtttt atcagaaaaa gcagtattta gttacaaatg tacaaattat tattacccag
    13501 aatttgagaa tggctttata tggaatgaca agcaaatatc tattgagtgg cctttaaaag
    13561 gaattgaaga aatattttta tcagaaaagg acaaaaaaca aaaaagtttt aaagatattt
    13621 gtacaagacg ttaataaaaa tttattagaa ttaacattta ggagaaaaaa tatgaaaaaa
    13681 atactgataa ctggaggagc aggatttatt gggagtaatt ttatacatta tatgcttaac
    13741 aagtatgtag attattttat tgttaaccta gacttgttga cttatgctgg taatttagag
    13801 acattaatag aaatacaaga caattccaat tataaatttg ttaaaggaga tatttcaaat
    13861 agggagcagg tgtataagct atttgaagaa gaaaggtttg atatagttgt taactttgcc
    13921 gctgaatctc atgtagatag gtcagttgag aatcctgatt tatttattaa aacaaacatt
    13981 ataggaacac aagtactttt agatgcatct ataaaatatg gagttaaaag gtatcatcaa
    14041 atatctacag acgaagtgta tggtgattta ccaattgata gaaaggattt attctttaca
    14101 gaacaatctt ctatcaatcc aagttctcca tattcagctt ctaaagcatc agcagattta
    14161 ttggtaagtt cttattatag aacatatgga ttgttaacaa ctatatctag gtgttccaac
    14221 aattatgggc catatcattt tcctgaaaaa ttgattcctc ttatgatttt aaatgctttg
    14281 gaaaacaaac aattgccagt atatgggaat ggggaaaatg taagagattg gttacatgta
    14341 tatgaccatt gtactgctat agacttaata atacataaag gaaatatagg ggagatttac
    14401 aatataggag gtcataatga aagaagtaat ctagaagtag taaaaatgat attaaatctt
    14461 ttaggtaaat ctgaagagtt aatatcctat gtaaatgata gacctggtca tgatttaaga
    14521 tatgctattg atgcaactaa aatagagaat gagttaggtt ggaaggcaaa atatgacttt
    14581 gatttgggta taaaagaaac tgttaagtgg tatatagaaa atgaatcttg gtggaaagct
    14641 gttttatcag tataacttta aaatttagaa aggggcgttt tatattgaat gagataagtt
    14701 cactaattaa tatattagca ttgactcaat tatctggtag taatactaca aatcaatgta
    14761 attgtacata taataattca tctggatttg atatgattat gatgaccttg ttaaaagcat
    14821 tatcacaaaa taatcaacat acttctaata gctacaattt attgtctaat tctgagaatt
    14881 tatttaatga acttgataat gtagtaaata ataccactag tagatttata gatgttgata
    14941 caaaagataa aaatgtaaag ttgagaattg aaaatgctgt agatcaagca tcaaaaaaat
    15001 ataacgtaga ttcaaatctt ataaaagcaa taattaaagt tgaatcagat tttaatccaa
    15061 ataccgtttc ttctgctggt gctaagggtc ttatgcaact tatgccagaa aactgcagag
    15121 acttaggagt tacagaccct tttaatatag aacagaatat agatgctgga actagacata
    15181 ttaaggaata cattgatatg tttggaggga gcatagaaat gggattgatg gcatataatg
    15241 gtggtcctgg aagaatgaaa agtagaggag tagaatcaat ctctgacctg tataaaatgc
    15301 caaaagaaac acaaaattat attcctaagg ttatgaaata ttatagaggg taggctttat
    15361 tatgggaata aatatagata agctaaacat aagtccaagt ataaatatgg aggttaaaaa
    15421 actaaaagag agtaataaat tttctgacaa attaaactct ttaaaacaat cttcttcatt
    15481 ggaagatata aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc ttgtacttac
    15541 tcaaaactat ggcgatatta gtatatataa aaatgcagta aaagaatacc taaaatctgt
    15601 tgtagataat atgtattcac ttgataaaaa tagtagcttt tgggagcatc agtattataa
    15661 gaatgtaaag attgttgata gtaaacttga ggatatgact agtaagttaa ttagtgaaga
    15721 aaaagaaaac atagatattg tttctactgt tgatatgata caaggcttgt tgatagatat
    15781 gtatagataa ggttatacaa ggagaaaagt tttatgaata gcaaaaaaga ggatatatat
    15841 gaattgaatt ttgggcaatt aatagaagaa aaatctggtg ttatagaaga aaatcaaaat
    15901 gtttttaatg caaaagtaaa tgtagctgtt tctataggaa atacaacaga aacaataaaa
    15961 aatatattag atttaaaaga aggctctata ataaaattaa gtaagaatgt ggatgaaaaa
    16021 ttagatatat atgcaaatga tagattgttt gcttatggag aaagcattat tacaaatgag
    16081 aagttatctg taagattatc taaaatagaa gattcagatg taaattagca ttttaaatag
    16141 tatcccttta ggctattctg tatgagttta gctaagggga tatttttata aaaaatttaa
    16201 aatatgtgtg aattatataa atgtttttat tttatgagcg ataactaaat tataagtatg
    16261 taattttgga ggtgaaacta tggatattaa aaatgtaagt tcaaatattg tagtgaataa
    16321 ttatttaaag aatacatctg gtataaaaaa agtggaagag gttaaaaaaa ttaataaaac
    16381 agacaagata gagttatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga
    16441 tgagaatatt agaaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta
    16501 caaaataaaa ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca
    16561 tgattacaga cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg
    16621 aattattaaa agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt
    16681 taaatcctaa actagaagat attagcaggg agcttgcatc catagaaata aagagaagac
    16741 aactttttgg tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatggatact
    16801 taaaagaaat atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat
    16861 caaatgattc tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa
    16921 aaccagctga taagcaaact acaacttata attcttatgg aaatatcaga aaataaatat
    16981 taggaggaga aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa
    17041 tgcgcaccaa aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg
    17101 ttattctagg caaaggaccg agtttgccac aaatagtcca atgtatatgc ctagtttgag
    17161 taattcaatt gcaagaggac aattagggac tggagtacat gtaacggatg taacaagagc
    17221 tagaaatagt ttttatgatt accaatttag ggcagaagct cataagtatg gtaagacagt
    17281 ttcaaaatat gattattaca atactataga aactatacta aatgaaccat cagactatgg
    17341 aatatctgca ggtatagatg atttttttaa aggatgggag tcattatcaa aggatataaa
    17401 tagtgacagt aaaaaaagtc tagtggtaga aaacgcatct aatttagcaa ctttaataag
    17461 tgaaagttat aagaagcttg ataagttaaa agaagatata gattctaata tagacactga
    17521 gataaaacaa ataaatgaca tgttaaaaag tcttgaagat ttaaataaat ctattgatat
    17581 tatatctggt tctggctcta caccaaatga tttgcttgat gaaagagata gaattttaga
    17641 taacttgagt tttaaactag actttgaaaa tagtgatgtt aaaaatatgc ttgcagatgg
    17701 taaattagaa cttaatgaat taaaaaatcc agatggaact tggaaaacag gtatatcagg
    17761 gaccttacaa ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa
    17821 agatgtatct gatggattag ctaaacagat aaatgatgtt tataatacgt ctgcaggaat
    17881 aacagtaaca gattttttta taacttcaaa tgtagatggt gaagatatta taaagataaa
    17941 tccagctata aagagtaacc caaatgaatt aaagcttact gctgatgaag cttcaaaaat
    18001 tgctaagtta aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat
    18061 aagtgaccac tataaagcct ttgcagaatc aatcggttta gattctcaaa aagtaaatca
    18121 agatgaagca aatcaacgta aaattataaa taatattgat aactcaagaa tgagtgtttc
    18181 aggtgtatct ttggatgaag aaatgacaga gcttataaaa atacaacgtt catatcaagc
    18241 tagtgcaaaa gttatgtcaa cagctgtgca actactggat gttgtaataa atggaataat
    18301 ttaatagaag ggggaattta taatgagagt cagtacaggt atgatgtcta gcagctatct
    18361 aaactcacta caagataatc tacaaagatt ggataaggtt aatagacaag ttaatacaac
    18421 aaaagaaata aacaagctat cagataatcc atataaagca ataaagatat taaactcaaa
    18481 aagtgaaata aagactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt
    18541 agaaactact gatactgcat tagatcagct tggaaaatta ttatcagaca taaagaaaac
    18601 tttggtggca agtggtgatg gtgcatactc agatgatgaa ttaaagactc ttagtaatga
    18661 aactaatgaa aaaatgaaag aaattgcaaa tgctttaaat gcaacacatg aagggaagta
    18721 catattttca ggttcaaata cgggggtacc acctgttgaa tgtatagata atgctgatgg
    18781 ttcagtgagt ttaaagttta attctagtct aaatcttaat aaattaaatg actctttgag
    18841 tatggatata gctcaaggaa taagtgtaga ttataacgtt aaattgagta gccttggatt
    18901 tgacccaaca aaaactgacc catttgaaga gttaaataac atatcaaaaa aattagcaaa
    18961 tccagatgct gatgctataa aagagcttac aacaacttgt ttgggcgata tggaacaact
    19021 aatagaaaat acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc
    19081 tatgaaggaa aaaaacgatg atagtttgat acagttaaag gatattttgt cacaaaatga
    19141 agaaatagat tatggtgaaa aacttgttca attaaaggca gcagagttaa cttatcaagc
    19201 ttcactacaa acaggaggaa agttatttaa tgtatctata ttagattaca tataataaaa
    19261 taacgcagat atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa
    19321 agaatatgag cttttagata tagaagataa tgattattta aaagaattta attctactga
    19381 agaagaaggt ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat
    19441 agttttaaat caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtatt
    19501 atttaatata attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc
    19561 tatagtgata aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata
    19621 ttcaataagg catccattat tgagaggtga taatggatgc tagtaatttc aagaaaaaaa
    19681 gatgaagcag tacttatagg agaaagcata gaagtaaaaa ttgtaggagt agatggaaac
    19741 aatgtaaaac tagctatatc agcgcctaac aatataagta tattaagaaa agaaatatac
    19801 gaaaaagtaa agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca
    19861 ttaaaataat aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat
    19921 aaacaaaatg ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga
    19981 gcagtaaaat ataccaaaat agccaaaaca gctatcttag ataaaaatac acaaaaagcg
    20041 catagagagc ttataagagt tcaggatata tttacagaac taatggttac attagaccaa
    20101 aatgcaggtc aatgggcaaa ggatatgtac agagtttatg attttgtaag atatgaactt
    20161 tcaagagcta atattagaaa agacattcaa ataatagata atgtgttgcc tgtaatagaa
    20221 caaattaaag atacttggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac
    20281 aaataatgga attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga
    20341 tgcttgtaga tggaacagta aaatatacca atatatccaa agaagctctt ttaaataacg
    20401 actatttgaa agcacataat gaacttgtaa gagttcaaaa tatatttaca gaactaatga
    20461 ctacattaga ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta
    20521 taaaatctga attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat
    20581 tgccaatagt aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat
    20641 aattggatgg agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc
    20701 aggaaatttt gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt
    20761 tgataaagca aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt
    20821 tatacaagaa tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat
    20881 agtaagtgaa aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt
    20941 agcaaaaggc tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc
    21001 tgaaccagca aaatttacta ttaaattaaa ttcaagtgaa cctattgttc aacagtttcc
    21061 tccaaatgct agtggagcga gttctttaac tataggagat gtaaatatac caatatctga
    21121 acaagatact acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat
    21181 aaaggcttct tatagtgaga tgacaggtga attgattatt tcaagaaaac agactggttc
    21241 gtcatcagac attaatttaa gagtaattgg aaatgacaat ttagctcagc aaattgctaa
    21301 tgataatggt atcacattta taaatgatgc tggtggaaac aaagtggcaa atgtatatgg
    21361 aaagaatcta gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga
    21421 acaaaattca tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa
    21481 gttgacttct gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga
    21541 ttataataaa ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta
    21601 tccgcctctt acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga
    21661 aaagaaagct aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga
    21721 tattcaatct gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat
    21781 caatgaaagt gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc
    21841 aaaggctctt atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa
    21901 ttatgatgat aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag
    21961 ttcaacttct aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa
    22021 tgtatattca aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat
    22081 gaatgataaa gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca
    22141 gtattcttcc caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta
    22201 aaagaaaaat taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag
    22261 gaagaatata taaagataag tattaagtta ggtgaaagac aagagattat aaatagtgta
    22321 agtgaaattg atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt
    22381 gatagcagga taagagatat tttacaagga caattattag aagtaaaaaa agaattacat
    22441 gagtataagc ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta
    22501 aatattttta ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag
    22561 aattaataaa aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta
    22621 aaataatgag agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa
    22681 acgtaaatgc acaaagtaag tctatggaga agctatcttc tggtgttaga attaagagag
    22741 ctgctgacga tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt
    22801 tagaccaagc aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg
    22861 cattagaaga aactggtaac atactacaaa gaatgagaac tttatcagta caatcttcaa
    22921 atgaaactaa tactgctgaa gaaagacaaa agatagctga tgagttacta caattaaaag
    22981 atgaagttga aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa
    23041 gttctacaga aataagatta caagttggag caaactttgg aacaaatgtt gcaggaacat
    23101 ctaacaacaa caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag
    23161 caggtataac aagttctaca atagcaagct taaatgcaga tggaacttct ggaacaaatg
    23221 cagctaaaca aatggtatct agcttagatg tagctcttaa agaattaaat acatcaagag
    23281 caaaattagg ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta
    23341 tagaaaatgt tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg
    23401 ttaacttatc taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta
    23461 atcaacaacc acaaggagtt ttacaattat taggataaaa taattttgta tattggatat
    23521 agaagaaagg gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag
    23581 gaaaatttat atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga
    23641 aaaaacttta aaatcattaa ccacattaag aaattctata gaaagtgaac ttataatagt
    23701 cgatacaggt tctacagatg atactataaa aatatctaag aagtacacgg aaaaagtgta
    23761 ttttcataat tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa
    23821 aggtgaatgg ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt
    23881 aaaatttttt aaagatggtc ttgataaaaa atttaattct gcttcaatac aattaaaaaa
    23941 tctttactca tatgataaaa aattatatgg atattgttca gtgcttagac tttttaaaaa
    24001 cgttgatttt agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt
    24061 taataatgtt gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt
    24121 aaataaggtg aaaagaaatg aaaaactatt atttcaagag ttaaaagaga atgataattc
    24181 tccatataca aactatcaat tagggaaaaa ttttattata ttaggaaaat atcaggaggc
    24241 tttatactat cttgagaaat cttatgaatt atattctaaa ctagaattgg cacctggtta
    24301 cttgataaca agccttgtaa aaacatattt atatttagga aaacataaga agtgtgaaag
    24361 attgtgctta aagtatatta agaaagacat gaataatatt gatatatatt attatattgc
    24421 acaagcacaa gtagatttag gaaagtatga aaatagtata gattcgtata agagatatat
    24481 ttacttgtta gataattatg aaataagtac acaagcaaat agtttatttt cagacactga
    24541 tactgtaggg cttagagatg aggctattat tacactgata aagatttatt ataagcttga
    24601 aaaatatgag tttgtaattt cagagtataa taacattgaa gatgtggaaa agaaaaagaa
    24661 tgtgtacttt agcttattta tgtcactata taaattgaat agatttgaag atataaaaaa
    24721 ttattataaa gaagtttcaa attctaaaat tgaaaaaaat agtttttata gaaatataga
    24781 agaagttata aataatatta aagaggatga aaaagagtat gtatataaaa tcctttgtga
    24841 cattgaaggt aattatggca ttttaaatca aataaggata aataaatgtg tatctataga
    24901 taaatgtaaa gaaatactca atgaagaaaa ggaaataatt tatgcatata ttataaaaat
    24961 agcttttgaa gagaagattg atttactaga tattttttat gaaatggaat atgtatggat
    25021 agagaaatat ctaaaatatc ttttagcatt agaaagaagt tttaatttag tactatataa
    25081 gtacataatt agtcgtccaa atacatcaga tatggaaaag ataagagtat ataaagctat
    25141 atctaaagta gttttagaaa gtataagctt aagtgatgaa aaatataagg agatttttta
    25201 tctttatata atgtattctt ataagtacat aagatacata tatagcaatt taagtgattt
    25261 tgaattaata aaatttgttt gtaatgattt agataagttc actatagaat ttaagcatct
    25321 acttgatatt aaaaataaag taaataatgc taatgaaaaa ttagagtata tacatagtat
    25381 gaggaaatta ctaaatgagt atccttttta taataaaata ataaaaatac ttataggtga
    25441 attagaagaa aatataaaag aaagtgaaga atttaaggat ttaaaagata gttttattat
    25501 tagtatagaa aatatgattg aaactggcaa aataaatgat gcgaaattat tggtagatga
    25561 ctattcaaaa acatttgtgg atgatataaa gattttaaat ataaagggaa ttttatacat
    25621 gtttgaaaat aagcttgata aagctgattt tatgttaaaa aaggcattat cattagatgt
    25681 agaaaatgaa gatactatat ataatataga atacttaaaa agtttaagat aagtcaaaaa
    25741 taggagaatt gaagaatgaa tataccaata gtggttaatg aaaaatctaa agacataata
    25801 aaagattggc aatatacatg tacaaatgaa gatataaaat ttacaagcat aataatattg
    25861 acatataatc aaatagaata tactaaatta tgtattgaaa gtattaggaa atttacccca
    25921 aagaataaat atgaaataat aataattgat aataactctt ctgatggaac agttgagtgg
    25981 ttaaagggac aatctgatat aaaatctgtg tataataaca aaaacttagg atttccaaaa
    26041 ggatgcaatc aaggtattga gttagctact ggtgaaaaca tattgttgtt aaataatgat
    26101 gtaatagtta ctccaaattg gctctataat ttgaataaag ctttatggtc tcaagataat
    26161 ataggtgctg taggacctat tacgaatgca tgttcatatt atcagcaaat tgatgttgat
    26221 tataagtcaa ttgaggacat gtttatattt gcacacaaaa taaataaatc taatattaac
    26281 ctatggaaat atagattgaa gttagttgga ttttgcatgt taataaaaaa aactgttctg
    26341 gataatgtag gggttttaga tgaattattc acacctggaa attttgaaga tgatgattta
    26401 tcttttagga tgatagagtc aggatataag ttattattat gccaggatac ttttatacac
    26461 cattttggaa gtgtgtcttt taaagaaaat cgtaataaat atacagatat aatgtatatt
    26521 aatagaagaa aatttgaaac taagtgggga tttaatccag aatatagtgg aatgataaga
    26581 acagatttga tttctaaaat agatgatagt ttggaggcaa atattaatgt tttagaaata
    26641 ggatgcggta taggggctac tcttttagaa ataaaaaata tatataaaaa tgcatcaata
    26701 tatggaatag aaattagtga gtcagcaggt aaacttgcaa agtatagtgc agatgtactt
    26761 atagcaaata tagaaaaagt aaaattggat tataaaaaag atttttttga ttatataata
    26821 ttgggagatg tacttgaaca tttgattgac ccatggaaga tagtcaatga attgaaacaa
    26881 tatctaaaga aggatggtta tctggtatct agtataccaa atattatgca tgtttctgtt
    26941 ttaagaaatc ttgtgttagg taattttaca tatgaagatt caggaatttt ggacaagact
    27001 catttaaggt tttttacatt aaatgagata acaaaaatgt ttaatgatag taattacata
    27061 attaatagta ttacagctac tagtatgcca ctttcggaaa ttgataatag cttcattgat
    27121 acaatatgta aggaaagctg taaagaaatg agacaacagt acgaatctta tcaatatata
    27181 gtaaaagcct ctaaaatcct agatactaaa agatattcta gtattagtat gattaattta
    27241 aaatacgctt taatgagaat tgataatgaa ttagatgtag atagtaattt aagttatata
    27301 tttgataatt ataatataac tgaaccatgt tttgttgatg atattgaata tctaattgat
    27361 acatttgtga taaataaagc aactctatta aatagattag gagttgaggc atttaacaga
    27421 ggtctaggag attttagtat aaacatgttt atgacagtat tagagatgga tagagatgat
    27481 attgactcaa tatataatat aacaagtgta ttatgtgaat tagaagaatt tgatactgct
    27541 tataatttaa ttaaaaatag tagcgaaaaa gtacagagtg aaagtgatat acaagagata
    27601 ctttgtttta ttgaggggaa gaaatatgaa taatgataag atatgtttta taacctgtgt
    27661 aaatgatgaa aggctttatg aagaatgtct tgtatatata aatagcctag acattcctaa
    27721 aaactttagt atagaaacag tagctttaag aggagccgat tctattgcct ctgcttataa
    27781 taaagctatg agagaaacag attctaagta taaggtatac ttacatcaag atacttttat
    27841 aattaataaa aatttcatac atgatatttt agagatattt aaaaaaaata cagatgtcgg
    27901 aatgattggt gtagctggag taaaagatat gcctgtgaat ggaatttggt gggattctga
    27961 taaaaaagca gggaaagtat atgatagtca tacaggtaaa atggatattt atgaatttag
    28021 taactttgaa gaatgtttta atgaagttat ggctatagat ggattaatta tgattactca
    28081 atatgatgta ttttggagag aggataagtt tgatgggtgg catttttatg atttgtcaca
    28141 gagtatggag tttagacaaa ataaatacaa agttattgta ccaaaacaaa ataagccttg
    28201 gtgtatgcat gattctggag tagtaaatat aaataatgga tatgatgaat ataggataaa
    28261 atttgtaaaa gagtacataa atcttgattt atatagagaa aatttttatg aatttgataa
    28321 gaagaaagca agtaccatac ttaagagtaa taagcatgta atggattgta tgaaagagga
    28381 gcttacagaa caattaagat taaagaatta caaggatgtt ttaggtattg tttctaaaat
    28441 tgctcatttt tctgttttta atcatactgg tttgtattct tgtgaagaat gtgagagagc
    28501 attgctagaa tgtgcaaata atttaccaga aatagaaaat acgaaattat ttacaaaaaa
    28561 agatttaaaa agaagaaaag tattacatgt tttaagtgaa ggatattcaa caggaggtca
    28621 tacaagacta gttaaaaatt ggattgaaat tgacaaagac tctgttcatt cagttgtaac
    28681 tacatggcaa tcagatacac tgccagactg gttattaaaa gcaataaatg atagtggtgg
    28741 cgaagttata tctttggatt taatttcaga tgattattta aaaagagctg tagctttgag
    28801 gaaaatagct tatgactggg ctgatatagt tattttatat gttcatatgt gggatacaat
    28861 acctgttgtt gcttttggag ttgatgggaa aatacctatt ttacatgtta atcactatga
    28921 tagtactttt tggttaggga caagtattgc agatattgtc attgatttta gcgaaactag
    28981 tcaattaatt acattagata aaagaggcat agataaatct gaaatattac caatgccatt
    29041 aaaagaaaaa gaagtatttt caaaatctga agttagaaaa cgatataatt tagaggatga
    29101 tgtgacaata atattgacta ttgctagtga aagaaaatac aatagtgtaa atgatattaa
    29161 ttactttaaa attgctaaaa cattgattga aaatacagat aatacaatta ttattgttgt
    29221 aggagttgat acagagaatc tctattggaa aactttaagt aatgttacag ataatagagt
    29281 tatatgtttt ggaacaacag caaatataga tgatttttat aagatttcgg atatatacct
    29341 agagtctttc atggtaggaa gtcatacttc aaaattagat gcaattttat atgatttaat
    29401 tcctgttaag tttaaaaatg aagtttcacc aatttttaca aatatgtgga attttataga
    29461 taagtttgat tataagaata taaaagaggt tatagacatg ataagaaggt acaatactag
    29521 tgaatttgac agggataaga taaaagcaaa acaatcggaa ataaaacatt ttgttataga
    29581 taaacattgt attgaaataa aaaactatat agacaaaata tataatgaag ttaatcatca
    29641 taaagttaag tatgatttat ataaaaatga tgaaatcgag gactatcagc tttttgtagc
    29701 attatatggc tatatggtaa aaaataaaaa catgtcatag gtggtaaaat gaacaatatt
    29761 catatcaata aatttgaaaa aagctgtgaa aaaaactggg attattttgt agaaaataaa
    29821 tctataaatg gtacatttct gcaaactaga aatttcctta attatcatga ggagggaaaa
    29881 ttcactgacc actcattaat aatatgtaaa ggtgaagaaa tactagcagt cgtacctgca
    29941 tgtgaaactt atgaagatgg tgaaagaata tttttttctc atttagggag tacttttggt
    30001 ggcattataa taagaagtgg ttttaatgat attaggcatg taaatgccat tacaaataag
    30061 ttagaaatat atttacagga acaaaatttc gataaagtat atttaaaaaa tacatcaaga
    30121 atattttgta aagaggatac ttcattacta gaatattttt tatttaagaa taattacatg
    30181 ttttatgatg aattaagtat gtttttagaa ttaagtaagt ataaagaaga tatattagcc
    30241 aattttaaat acagtaaaag aagatatttt aaatattctt tgaagaataa tcttaccttt
    30301 aaaagactgt atagtgatga agatatagtt tgttttttta atttactttc taattgttta
    30361 gatagtaaag gagctaaagt aatacattca gtagaagaac ttttggagtt taaaaataat
    30421 agattaaaag atattgtaga attttatgca gtatatattg atgacattat gatttgtggg
    30481 agcatggtat ttaactttca agaaagagtt tatcatactc aatatctagc atcaaattcg
    30541 acatataata aatattatcc aatgatattt ttatactata atctaataaa agatgcttta
    30601 gagagaaatt ttaagtatct ttcttttgga attagcactc taaatagagg caaatactta
    30661 gatgagggtt tagctacatt taaagaaggt tttggttcaa gtgcaatttg caacagaaca
    30721 tactataaaa atataaataa gtgagaatca tgttttatag agtttttaga gtatagtaaa
    30781 tatctagtac tctaatatta cataaaaata attagtttat aaagaaggtt tgtatatgaa
    30841 aagattaatg attttaggct caattaagta ttttgagaat attgttatgt cagctaaaaa
    30901 agagggaata tacacaattg tttgtgataa tagaattgat actcctgcaa aaaagatttg
    30961 tgatgaagca atagatgttg atgtatttga ttttgataaa ataaaaagta tagctataga
    31021 taaaaagata gatggaattt taactggatt tacagattct ttaatgaaac catatgttta
    31081 tattgcaaat gaactaaatt taccatgtgt gatttcttgc aatcaacttg aatctgtaac
    31141 taataaggct attatgaaaa aatattttaa aaataatgca atacctacaa ctgattattg
    31201 tattattgaa agtctaaaag atttagaaaa aatacaagga ttaaattttc cgcttgtaat
    31261 aaaacctgtt gatggttatg gttcaagagg tgtatatttt gtatcaaata tagaagaatt
    31321 aatagaaaaa tttgaatatt ctaagaaagc ctcacaagat ggacaagtaa taattgagga
    31381 attttatgaa agtgaggaaa tacaaggact agcttgggtt cataatggag agtcacatgt
    31441 attttatatt ggagatagag agcttgtaaa tatacataca ggaagagctg gaaaaccaga
    31501 tagattactt tatccatcaa agtattgttt tcaatatgag gaggaaataa aatctatata
    31561 tcaaaatata acacaggttt ttgatataaa aaatggacct ctttatgtac aaatgttagt
    31621 gggagcatat ggaataaagg tatctgaagt aatgccaaga ttacctggag gttgtgacta
    31681 tatggctatt tctcaaataa cagatttaga cataggaagc ttatttgcaa actttagtgt
    31741 gaataatgaa gtaaattttg atgaaataaa gaaatataat atgcatctat caaaatgtgt
    31801 atatgcatta ccaatataca taagacctgg gacaataaaa aagataaata atatagataa
    31861 aatagaaaac ttagattatg tcactcaatt tctattacta gtaaaagaag gagatacaat
    31921 aggattatat gaagatgaga gacaggattg tggaagagtt tatggaatag caaataatat
    31981 atttgatgca aatataaaaa agaaatgtat tcatgaaatg atagaaatac ttgatgaaaa
    32041 taatgaaaat atgatagaaa atttttaaat aagggagtat gatagatatg aagctaataa
    32101 ctcatgaaca aattatgtca ttaaacattt ctcagaaaca aggatatgag tggataaaag
    32161 aatccttctt aacaaaaaaa gattgtcaat taccaccaaa aataagcatg gtagaagaag
    32221 gacatgtatt ttacaatgta atgccatccc taaatcataa tcatatgatt ggtggaataa
    32281 aaatggttac aagattgcca gggagaaatc caagtttaaa ctcagagata tttctatata
    32341 atatggaaga tgctaaatta aaggctattt tggatggtaa ttatattaca gcttttagga
    32401 ctgctgctgt tgctgccttg gctattgata catttgctaa aaaagatttt aatactattg
    32461 ctattatggg tttaggtgta actgcttctg ctataatgaa tatgctttcg tatactctta
    32521 aagataaaaa tataaatata aagttactta aatataaaga ccaagcagag tcatttataa
    32581 aaaagtatga aaaaaatgaa caatttagtt ttgaaatatg tgatacacat gaagaactta
    32641 ttgtagattc agatgtagtt atttctacag tgacatatac agatgaatat ttatcagaat
    32701 ttaaatggtt taaaaaaggt gctttattat tacctgtaca tgttatggga tttggaaatg
    32761 ttgattatct atttgataaa gtatatttag atgattataa tcatttgaaa catctggaaa
    32821 atatagatag atataaatat tcttgtgaat tttatgatgt tttaagtaaa caagctaaag
    32881 gaagagaaaa tgatgatgag agaataatat cttacaatat aggtttgtca atacatgata
    32941 tattatttgc aaataaaatt tacgaattgt gtaaaacact aaattgtgaa gagatagaat
    33001 taaaagaacc aaaagaaaaa tattggatat agtttatttc attcatattt acaataattt
    33061 attgtaaata tgaatatgaa gtaatatatt gttagaaaat tagcatgaat aggatagata
    33121 aatttatgga taaatataat ttaattaagt tcgtatctat tggaaacgaa tatataggaa
    33181 atttagttgc attagaatca aataaagcaa taccttttga tataaagaga gtttattaca
    33241 tatatgatgt accaaacaat gtaaagagag gatttcatgc acacaaaaac ttggaacaag
    33301 tactagtttg cctaaatgga agtgtgaaga taaaatgtta tgatggtgta aaagagacta
    33361 tatttaattt acataaaaat gatgttgggt tgtatatagg aatgggagtg tggcatgaga
    33421 tgtttgattt tgatgaaaaa actgttttaa tttctatagc atctgaacat tataatgaag
    33481 aagattatat tagagactat aaagaatttt taaagtattt aaataatatg tagttattat
    33541 acagttagtg atgtattttc ttaattaaat aaatttaaga tagccatata gagaataaat
    33601 agattaggat gtgaatttat tgttggttaa atttaataat ttattaaaac agttccaaac
    33661 tcataaagaa gaatatgaac agaagattaa ggatgtaatg gaaagtggat attatatcct
    33721 tggaaatgaa gtaagacatt ttgaagaaga atttgctagc tatgtaggta gcaaatattg
    33781 tgttggttta gccagtggtt tagatgcact gataattgct tttagagcat taggaataag
    33841 cgaaggtgat gaagtaatcg tgcaatcaaa tacatatatt gctagtgtta tgggaatcag
    33901 tataaatgga gcaacaccag tatttgtaga accaaatgaa tattataata tagattcatc
    33961 aaagatagaa aaaaagataa atagcaaaac aaaagctata ttagtagtgc atttatatgg
    34021 tcaagcaagt aaaatgaaag atatacaaga attatgtgat aaatataatt tgtatttaat
    34081 tgaagattgt gctcaatctc atggagctaa atatgatgga aaaatgacag gaacatttgg
    34141 tgatataggt tgctttagtt tttatccaag taaaaatttg ggcgcatttg gagatgcagg
    34201 agctatagta actagtaatg aggaaatagc tgataaagtg aaaattctta gaaattatgg
    34261 ctcagaaaag cgatatcata ataaatttgt cggatataat tcaagattgg acgaaattca
    34321 agcaggttta ttaagagtaa aacttaaata tctaaatgaa attgaaattg aaagatcaaa
    34381 aatcgtagat agatatctta atgaaattaa taatccaaaa gtcatacttc caaagataga
    34441 aaataattgt actcatgtat ggcatttgtt tgttttacaa gttgaaaaac gagatacatt
    34501 tatggaatac ttaaataaaa atggtataga gtctataata cattatccga taccaccaca
    34561 tctatctgaa gcatatgctt atttgggata taaaaaggga agctttaata tagctgagaa
    34621 ttattcagat aaagttgtta gtattccttt atttataggt atgaaagatg aagaagttag
    34681 ttatataatt aaaattataa atgaatatta aactaatcat aataatatca aaaataaatt
    34741 ttttatatat cttttattta agt
//
LOCUS       DAELJO010000018        34613 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELJO010000018 DAELJO010000000
VERSION     DAELJO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.018"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            209..1330
                     /locus_tag="KPB88_000804"
     CDS             209..1330
                     /locus_tag="KPB88_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053106.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            1348..2223
                     /locus_tag="KPB88_000805"
     CDS             1348..2223
                     /locus_tag="KPB88_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG0053107.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            2247..2939
                     /locus_tag="KPB88_000806"
     CDS             2247..2939
                     /locus_tag="KPB88_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0053108.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            2947..3771
                     /locus_tag="KPB88_000807"
     CDS             2947..3771
                     /locus_tag="KPB88_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0053109.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            3776..4843
                     /locus_tag="KPB88_000808"
     CDS             3776..4843
                     /locus_tag="KPB88_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG0053110.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            4845..5381
                     /locus_tag="KPB88_000809"
     CDS             4845..5381
                     /locus_tag="KPB88_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0053111.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            5449..6054
                     /locus_tag="KPB88_000810"
     CDS             5449..6054
                     /locus_tag="KPB88_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG0053112.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            6066..6980
                     /locus_tag="KPB88_000811"
     CDS             6066..6980
                     /locus_tag="KPB88_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0053113.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICINNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            7022..8194
                     /locus_tag="KPB88_000812"
     CDS             7022..8194
                     /locus_tag="KPB88_000812"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG0053114.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            8241..9053
                     /locus_tag="KPB88_000813"
     CDS             8241..9053
                     /locus_tag="KPB88_000813"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG0053115.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQIVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            9257..10582
                     /locus_tag="KPB88_000814"
     CDS             9257..10582
                     /locus_tag="KPB88_000814"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG0053116.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVVHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            10795..11874
                     /gene="mltG"
                     /locus_tag="KPB88_000815"
     CDS             10795..11874
                     /gene="mltG"
                     /locus_tag="KPB88_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG0053117.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            12046..12720
                     /locus_tag="KPB88_000816"
     CDS             12046..12720
                     /locus_tag="KPB88_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG0053118.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            12707..13954
                     /locus_tag="KPB88_000817"
     CDS             12707..13954
                     /locus_tag="KPB88_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0053119.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            14026..15690
                     /locus_tag="KPB88_000818"
     CDS             14026..15690
                     /locus_tag="KPB88_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG0053120.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(15787..16428)
                     /gene="sigK"
                     /locus_tag="KPB88_000819"
     CDS             complement(15787..16428)
                     /gene="sigK"
                     /locus_tag="KPB88_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBG0053121.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            17020..17382
                     /locus_tag="KPB88_000820"
     CDS             17020..17382
                     /locus_tag="KPB88_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053122.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            17366..18073
                     /locus_tag="KPB88_000821"
     CDS             17366..18073
                     /locus_tag="KPB88_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053123.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKKNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            18093..18512
                     /locus_tag="KPB88_000822"
     CDS             18093..18512
                     /locus_tag="KPB88_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053124.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            18502..19026
                     /locus_tag="KPB88_000823"
     CDS             18502..19026
                     /locus_tag="KPB88_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053125.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSTTNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            19338..19895
                     /gene="efp"
                     /locus_tag="KPB88_000824"
     CDS             19338..19895
                     /gene="efp"
                     /locus_tag="KPB88_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG0053126.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            19987..20442
                     /locus_tag="KPB88_000825"
     CDS             19987..20442
                     /locus_tag="KPB88_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0053127.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            20605..21315
                     /locus_tag="KPB88_000826"
     CDS             20605..21315
                     /locus_tag="KPB88_000826"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG0053128.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            21369..22439
                     /locus_tag="KPB88_000827"
     CDS             21369..22439
                     /locus_tag="KPB88_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0053129.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            22467..26021
                     /gene="smc"
                     /locus_tag="KPB88_000828"
     CDS             22467..26021
                     /gene="smc"
                     /locus_tag="KPB88_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG0053130.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKNMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            26048..27328
                     /gene="ftsY"
                     /locus_tag="KPB88_000829"
     CDS             26048..27328
                     /gene="ftsY"
                     /locus_tag="KPB88_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG0053131.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            27431..27799
                     /locus_tag="KPB88_000830"
     CDS             27431..27799
                     /locus_tag="KPB88_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG0053132.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            27796..29148
                     /gene="ffh"
                     /locus_tag="KPB88_000831"
     CDS             27796..29148
                     /gene="ffh"
                     /locus_tag="KPB88_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG0053133.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            29186..29458
                     /gene="rpsP"
                     /locus_tag="KPB88_000832"
     CDS             29186..29458
                     /gene="rpsP"
                     /locus_tag="KPB88_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG0053134.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            29523..29750
                     /locus_tag="KPB88_000833"
     CDS             29523..29750
                     /locus_tag="KPB88_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG0053135.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            29858..30373
                     /gene="rimM"
                     /locus_tag="KPB88_000834"
     CDS             29858..30373
                     /gene="rimM"
                     /locus_tag="KPB88_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG0053136.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            30382..31077
                     /gene="trmD"
                     /locus_tag="KPB88_000835"
     CDS             30382..31077
                     /gene="trmD"
                     /locus_tag="KPB88_000835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG0053137.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKYIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            31262..31612
                     /gene="rplS"
                     /locus_tag="KPB88_000836"
     CDS             31262..31612
                     /gene="rplS"
                     /locus_tag="KPB88_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG0053138.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            31933..32829
                     /gene="ylqF"
                     /locus_tag="KPB88_000837"
     CDS             31933..32829
                     /gene="ylqF"
                     /locus_tag="KPB88_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG0053139.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPDKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            33218..34510
                     /gene="brnQ"
                     /locus_tag="KPB88_000838"
     CDS             33218..34510
                     /gene="brnQ"
                     /locus_tag="KPB88_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0053140.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        14363 a         3354 c         6390 g        10506 t
ORIGIN      
        1 acagtggttt attttttaat ataaatattg actttgatgc aggtaaactt tgtattttta
       61 agcgtattat ggaatatgtt aaataaaaaa atgatgaaat atagtattgt aaatgccaaa
      121 gatgcaaaac aaacttaaaa catttatttt attgttaagt aatgctataa tataatgtga
      181 ttttaataat gatagtggag gtttaaatat gagagtcgag gcccctataa aagtagatcg
      241 aaaaaccaaa aaacttgcta aaagagttga aagtggggaa atagcagtta taaatcatat
      301 agacatagat gaagttgctg caaactcttt agtagaagct aaaataaaac ttgtcataaa
      361 tgcggctcct tctataagtg gtaggtatcc caataaaggt ccagggatat taactgaaaa
      421 taatatacta ataattgata atgttggaga agagttattt gaagaattaa aagaaggaga
      481 aactatagaa gttgtagatg gaaagatata tagaaaagga aaatttttag gtgcaggaga
      541 agttttagac aagtatgagg tttcacacca aataaaggct gcctatgaga atttagctgt
      601 agaattggat agatttatag ataatactat agattatgct aaaaaagaaa aaggatttat
      661 acttggagaa gtagaaattc caaaagtgaa aactaattat gctaataaac atgtattgat
      721 agttgtaaga ggtcaggact acaaagaaga tttaagtact atgctttctt atatagaaga
      781 ggtaaagcca gtattggttg gtgttgatgg aggagcagac gctttaattg agtttggata
      841 tactccagat gtgattgtag gtgatatgga tagtgtaagt gatgaagctt taaaaaaggc
      901 tagtgaaata gtagtacatg cttatacaga tggaagagca ccaggtctta aaagggttga
      961 ggaattaggc ttagatgcag ttgtatttcc tgcgccagga actagtgaag atatagctat
     1021 gcttatagcc tatgagtata aggctgagtt aatagtagca gttggtacac attcaaatat
     1081 gattgatttt cttgaaaaag gaagaaaagg tatggcaagt acttttttag ttagactaaa
     1141 aataggttca aagcttatag atgctaaagg cgttaatttg ttatacagaa gtaaattaaa
     1201 aataaagtac atatgggcat taatagcaac agcattattt cctgtgttgg tagttgcatc
     1261 attgtcacca ggagttcaac agttcataca actaatgcaa ttaaagttta gagtattatt
     1321 acagatgtaa attttaggag gataaatatg catattaata tgaaatatta tatagtcact
     1381 ataggggcta tatttatagc tttgggtata ggcatgttag taggatttaa tttaaataat
     1441 aatcaacaat taagtgaaca acaagcaaac ataataaatg atttggatga taagtttaat
     1501 atcttaaaag aaaaaaatga taaacttgac agtgacttag caagtgtaaa tagagattat
     1561 gaagaggcag ttaattttat aaataaaaat gtagataaga ttttagctgg ttcgctgaat
     1621 ggtaagagta taggaataat atctgctaat gaaaatgatg attatacaaa aaatatagaa
     1681 gacataataa ataaatctaa tggaagcatt gcatttaata taatattaaa agaaaacata
     1741 acaaatccag aaaagttaaa ggaaatgtca actaaactag gtacagatgt aaaaaatgca
     1801 aatgatgcag ttaattatat aattgacact cttaaaaagg aagatgcaag tgacatttta
     1861 acatatcttc aagaactaga tgtaataaag tttaatttta ttggagatac atatctaaaa
     1921 tatgattccg ttgtaatagc tggtggaaat gatgcaaaag acagtgcaaa acagtttgaa
     1981 aaaatagaaa aatttgtagt ttcaaaactt aaatcagaga ataaatattt agtagaagtt
     2041 caaaatacag gagttaaaac ttcttatgta gaactatatt ctaaaaacaa agttgcaact
     2101 atagacaata ttgatgaagg tataggaagt atatctctag ctattcttct acaacaaggt
     2161 aatatagttg gtaattttgg tagattggat actgcaactt cattgttgcc atctattaaa
     2221 tagaaataat tttaaggaga gaaaaaatgg attcttatat aagtataata ataccagctt
     2281 ataatgaaga aagtagaata aaatatacat tagaaagcat attggacatt aagcaaataa
     2341 atgaaataat agttgtggat gatggttcaa ttgacaatac tgctaaggtt gtatctgagc
     2401 tagaaaatga aaagattaaa ttttttaagt tagataaaaa tagaggtaaa ggatatgctt
     2461 taaattatgg tatcaaatta gcaatgaaaa atgcagatat tataggtttt ctggatggag
     2521 atttaggaag ctctgctaaa gaagttgaaa aattaatagt acctatatta aatggtgagg
     2581 cggatgttac aatagccaag tttcctcctg caaagagaaa aggaggtttg ggatttgtaa
     2641 aaggtttagc taaaaattct gtgtttgaga tgactggtgt tgaattagat gcaactttgt
     2701 caggtcaaag aatatttaaa agagaagttt tagaaaagtt tgatgaaata ccttttggat
     2761 atggagtaga agttggaatg accattgata tattaaaata tggatttaaa attaaagaag
     2821 tattagtaaa tatgactcat aatgaaacag gaagaaattt aaaaggtttt attcatagag
     2881 ggaaacagta ttatcacata aaaaaggttt taggacaaaa aaagaaagaa tggaggtaat
     2941 atcttagtga aagattatat actttatacc attttattat taactggatt atttggaacc
     3001 tatgctgtaa ttccactatt taaaaattta ttgataaatg gaaatgtact tagaccaaac
     3061 tataaaaaag atatgatacc agtaagtatg ggaattgtat ttttgcctat gattgtaatc
     3121 aatggaataa tacttgcttt ctttactaca gaatttaagg atttagcgta tatatttatg
     3181 tttatgtttg gtatgatatc catgtttttt gcagggatac tagatgatgt cattggaaat
     3241 agagatgtaa gtggtttaaa ggggcatttt aaaagtttat ttaaaggaag tcttacaaca
     3301 ggaggtttta aagctttatt tggtggattt gtagggctta tagtttctgt tgctatatca
     3361 aaagacataa ttgatataat agtaaataca ctaataatag ctctatcaac taatttaatg
     3421 aatcttttgg atttaagacc aggaagggca ataaaagcat atttattcat aatggtaatt
     3481 atatttttaa cattgacagg atttgttcaa gttcttccat tacttatagt accaaatgta
     3541 cttgcatact ttaattatga tttaaaagca agggctatga tgggagatac tggttcaaac
     3601 gtccttggaa tatctattgg aatgctcatt tcttttgggt atccatttaa cattagatta
     3661 ggatggttaa tatttgtagt tattatgcat atattcactg agaaatactc attaactaag
     3721 ataatagaaa aaaataaact attaaatttt atagataaat tgggaaggta gtattatgat
     3781 aagcaaaaga gtggggattg ttgaatcaat agtaagtcaa actgaaactc tagatgatat
     3841 aagagtaaat ataaatggag aaatacaaaa ggcatataat tatccaaaaa tatctggaac
     3901 tataaatata ggtgacgaag tggttttaaa tacaacagca gtagaactga gcttaggtac
     3961 aggtggatac cattttgtaa taactaattt aaataatata gaatctactt taactgaagg
     4021 tggacatata atgaaactta gatatacgcc tcttcaaata aaagtggatt ctgttgagga
     4081 acaagaaagt atttatcatg ataaatttgc gaattttaaa ggattagaag gtctttctgt
     4141 tgtagtaggg actcttcata gtatgttaac tccatttgca gcttcattta aaagaaataa
     4201 tccaaataaa aaattagtgt atataatgac tgatggagca tctcttccaa tttatttaag
     4261 taaaaatgtg gatactttaa aagaaaaaaa attaatagat agtacaataa ctataggaaa
     4321 tgcgtttggt ggagattatg aatgtataaa tatatatact gcattaatta cagcaaaaga
     4381 aatactaaaa gcggatgcag tatttgtcag tatgggacct ggaattgctg ggacaggaac
     4441 aaaatatgga tttacaggga ttgaacaagg ctctattctt gatgctgtaa aaaaacttga
     4501 aggaagagca attgcaattc caagaattag ttttgctgac aaacgagaaa gacatcaagg
     4561 tatttcacat catagtatga ctgtatttaa agaaattgta aatgtaaatg tagacatacc
     4621 aataactata tatgatgatg aaaaattgaa ctttataaaa gagcaaatca atttaaatgg
     4681 tttagacaaa aagcataata taatttatat agataataat aaaacgaaag aggatttaga
     4741 ctattttaat ctaaaagtaa agagtatggg aagaaattat gaacaagatg aggcattttt
     4801 caaagctgca agcacagcag catattactt aatggaggtt taagatggta ttagaagaaa
     4861 aaactattag tagtgatagg gtttatacag gtaaagtaat tactttaaag gttgatactg
     4921 ttgaaattcc agggcaagga tatcaaaaaa gggagttagt agaagttggt ggagcagtag
     4981 gaatagttgc aatcacagat gataataaag ttgtattagt taagcaattt agaaaaccta
     5041 tagaaaagcc aatctttgaa attccagctg gtaaactaga aaaaaatgaa agtccaaaag
     5101 aatgtgctga aagagaatta aaagaagaaa ctgggtatag tgcaaaaaat attaagctaa
     5161 tacataaatt tttcacttca gcaggttttt ctaatgagat aatgtttgta tatttagcaa
     5221 caggtcttac tccaggagag aataatttag atgcagatga gtttttagat gtctatgaaa
     5281 ttgagcttga agaagcatat aacatggtat taaaaaatga cgttgaagat gcaaaaacat
     5341 ctattggatt attattagta aaagatatgt ttaaaaatta atatttataa aatgaataac
     5401 cctttgtcct aaagcatatt attttataaa ggacaagggg gaattaaatt gagaagaaca
     5461 tatagaaaaa atgattttag agaattaaat agacaaataa ttattatagg attgttgttt
     5521 atgatgtcta ttgttgttgg gtcttacata aataaaatat tgccaggttc tagtaacaat
     5581 atattaaaca atataaatcc tgctgtagag tattataatc taaatataag cataaaagat
     5641 acagtgatac aaaatttaaa atcagatgca atctttatgg gctcaatagc tctattaagt
     5701 ctatttgtag taacaattcc agcagtgttg gtagcttttg tattaaaagg gatgtcaatt
     5761 ggttatacaa taaatagttg tatattggca ttaaaattta aaagtataaa aatgatttta
     5821 ataatattat ttaaaaattt gattattatt ccatgtgcaa tcatattagc attaattagt
     5881 ttaagttatt ttaaagaaat ggtttatgaa tttaaaaaga aaaatagaaa aaatatgcaa
     5941 tttttaatta agaggtatat actaaatatt ataattataa tagcactatc attggggttg
     6001 caattaatat taaatactgc aagtattggt attattaagt tcctagctaa ataaaggagt
     6061 ttataatgga tattatagag ggatatatag attacataaa agataaaaaa aaattatcag
     6121 agaatacagt ggcatcatac tttatggata taaaaaaata tatggaatat ataaatcaaa
     6181 aaagcattaa actagtagat atagtagaaa atgatattat aagttactta ataaagttgg
     6241 aaaaagataa tgtatcaata gctacaatag cgaggatgat atcctctata aaatcttttc
     6301 atgattattt atttttaaat catatatgta taaataatcc tgcaaaggat ataaaaaaac
     6361 ctaaaataaa aaaagaaaat ataaatatac ttacagaaga agaaattgaa agactattaa
     6421 attttccaaa gttaacaacg cctaaattga ttagggacaa ggctattttt gaagtattat
     6481 atggcactgg tataaaagta tctgaattag tagagatgaa tatagaggat attgacctag
     6541 atattgatta tatatattgt aattcatgtt gtaaaaatca aagagtgata ccactttgtg
     6601 atattacaaa actttactta gaaaagtatc taaaagaagc aagacctaaa atggctgtag
     6661 aaggtgaaaa atcattattc gtaagttcat taggtcaaag gtttacacga caaggtcttt
     6721 ggaaagtcat aaaaaaatat tctaatctag ccaatataga taaaaatata aatcctacaa
     6781 tgcttagaca ttcatttgct attcatttgt taaatgaagg tgctaacatt gctgttgtaa
     6841 gtaaaatttt aggaaatgtt aatttatcta gtttgcaagt atatttaaat catatagata
     6901 aaaacgtgag aagagaaata aaggagaaac atcctagaaa tgatgttgat gtagaattac
     6961 aaaaagcaga agctaaatag actatatagc aaattttata atttagaagg agcatattta
     7021 tatgagcaga gtaatttgga ttgtaattga tagtgttggg ataggagcat tacctgatgc
     7081 tgaaaaattt ggtgatagta aggatgtaag tacccttgga aatatattta aggaatatcc
     7141 agatattcag attcctaata tgagaaattt aggtatagga aacatagatg gtatagattt
     7201 ttttgaaagt ataaaagaac caataggttg ttttggaaag tgtaaggaaa tgtctcaggg
     7261 aaaagatact actacaggtc attgggaaat gactggtata atagttgaca aaccatttaa
     7321 gacatttgaa catggattct ctaaggagat aatagaagag tttgagaaaa aaactggaag
     7381 aaaagtagta ggaaataaac cagcttcagg aactgtaata atagatgagt atggagagca
     7441 tcaaataaaa actggagatg taatagtata tacatcagct gatagtgtat ttcagattgc
     7501 tgctaatgaa gaagtaattc ccctagaaga actctataat atgtgcaaaa tcgctaggga
     7561 aataatgatg ggggataatg cagtagctag agtaattgct agaccattca taggtaagaa
     7621 aaaaggtgag tttgtgagga cttcaaatag aagagattat tcactagacc catttgaacc
     7681 aacagttctt gataatataa aagaatcagg acttgatgtt ttagcagttg gaaaaataga
     7741 agatattttt aatggaaaag gaattacaga tgctatccac actaaaagta atatggatgg
     7801 agtggatgaa actttaaatt atatgaaaca agataataaa ggtctaatat attcaaatct
     7861 cgttgatttt gattctaaat atggtcatag aagagaccca gaaggatata agaaagctct
     7921 tgaagagttt gatagtagac ttcctgaaat aatggctaat atgagagaag atgatatttt
     7981 aataatcaat gcagaccatg gaaatgaccc aacatataaa ggtacagacc atacaagaga
     8041 atatatacct gttatgattt atggcaataa gattaaaaaa ggttttaatt taggagtaaa
     8101 agatactttt gctgatattg gagcaactgt tgcagatata ctaaatgtaa agttgcctaa
     8161 acatgggtca agctttaaag aagatttatt ttaaattata aaatgataaa aacttagctt
     8221 aataggatgg agagattgga atgtttgaaa agatagcaca aagtagtaaa tttataaatt
     8281 ctaaaagtaa tataaaacct aagattggtt tgatattagg ttctggacta ggtgatttag
     8341 caaatgatat agaagaccct gtcacaatta aatatagtga aattccaaac ttccctgtat
     8401 caacagtagc aggacatgct ggacaaattg taattggaaa acttgaagga aaagaagtta
     8461 ttgctatgca aggtagattt cattactatg aaggatattc tcagaaagaa gctacttttc
     8521 cagtaagggt tatgaaagaa ttaggagtcg agattcttat agtaactaac gctgctggag
     8581 gcgtaaataa agaatttaaa gcaggagatt tgatgcttat aagagaccat ataaacttta
     8641 gtggaagtaa tccattagta ggaaaaaatg atgacagatt tggtgctaga tttccagata
     8701 tgtcggatgc atattcttct aaatatgtag atgtagttaa aaactgcgct aaagaatgta
     8761 atatagatgt caaagaaggc gtttatatgt tttttagtgg tccaaactat gaaacaccag
     8821 cagaagtaag aatggcacaa attttgggag ctgatgctgt aggtatgtct actgttccag
     8881 aagtcatagt agcctctcac tctaatatag gagttattgg tatatcttgt ataacaaata
     8941 tggctgctgg catcttaaat caaccactaa gtcatgagga agtaatagaa actactcaaa
     9001 aagttaaatc agaatttatg aatttagtta aaacaacggt taaaaattta taagttaaag
     9061 cttattatag attttttgag gaagtttata ataaaatagg taatgtttaa tttgaaaaga
     9121 attaataaaa agctgtcttg atatagaaag ttatttctaa taaaggacag tttttttata
     9181 atactacaac atttgaaagt gttttaaagt ttaatattta tttaaattat tataggttag
     9241 aatggagatt tcaattatga gaatgtatga tattataaaa aaaaagagag ataacctaga
     9301 gcttagtaaa aaagagataa actactttat agaaaactat tcaaagggag atatacctga
     9361 ctatcaagct tctgcactat taatgtcgat ttatttaaat aagatgaata aacaggaaac
     9421 tgtacatctt acagaagcaa tgatgaactc tggagacatg ataaacctat ctgagataga
     9481 tggaataaag gtagataagc atagtactgg tggtgttgga gataaaacta ccatagctct
     9541 tatacctttg gttgcttcat gtggagcacc agttgcaaag atgtcaggta gaggtcttgg
     9601 tcatactggt ggtactattg ataaactgga gtctatacca ggtttttcga cagaaatgga
     9661 gataagtaaa tttattgatt ctgtaaataa gtctaaaata gcagtttgtg gtcaaagtgc
     9721 aaaggtagca attgcagata agaaaatata tgctcttaga gatgttacag caactgttga
     9781 taatatatca ttaatatcat caagtgtaat gtctaaaaag ttagcttcag gagctgatgc
     9841 aattgtgcta gatgtaaaaa ctggtgatgg agcatttatg aagacattag aagagtcttt
     9901 tgagttagct aaatctatgg ttgatatagg tactggtatg aatagagata caatagggat
     9961 tgtaacagat atggatgaac ctttaggttt tgcagtggga aactctttag aagttataga
    10021 ggcaattgag acattaaaag gaaatggacc taaagacctt gttgagctat gcgaagtatt
    10081 aggagcatat atgttggttt tatcaaaggt agtacatgat tttgaagatg gtgttgaaaa
    10141 aataagagaa tcaataaaat ctggtactgc tatacaaaaa ctcaaagagt ttatagagaa
    10201 tcaaggtggt aataaagatg tagtagatga ttattcttta ttcaaaaaat caaagtataa
    10261 atatccagtt aaatcaacta aatcaggtta tataagtaaa attaaagctg aagatgttgg
    10321 tgtatcggct atgattcttg gagcaggtag agaaactaaa gatgatattt tagatttatc
    10381 agcagggata attttagaaa agaaagtagg agattatgta aatgaaggtg aagttttagc
    10441 ttacatgtat tataatgatg aacaaaagct ttcattggca aagggaagat ttattgattc
    10501 ctactctatt gtagattcaa aaatagaaaa aaataaatta atatatggta ttgtaactaa
    10561 agaagagatt aaaaagtttt agaaaatttt agaaaagtga ttataaaagg cctaacatgt
    10621 caaaaataat gttaggtttt ttattttttg ataaactata aattatacca aaaatatata
    10681 ttttaaaaca ttttttacaa tataaataag tttattctac aagatgattg aaaattaaag
    10741 tatctagttt tataatagaa aatggataaa tatacataag gagtagatta tattatgaac
    10801 tttaaagaaa atagattgaa aatagcagta ttaataattg ttattttgat tatcttagca
    10861 ggtatatttg tgtttataca aataggtcct tatgataaaa ataacaaaaa agatgtaatt
    10921 atcgacgttc caagtggagc atctgttgga aaaatatctg acatattata tgaaaataaa
    10981 ttaataaaga atgaactatt atttaaactt ttggtaaagg ttagcaataa agctccgtct
    11041 ataaaatcag gaacatactt acttaatcaa tcgtattcaa acaatgatat aataagtctt
    11101 ttagtttcag gtaaaatata tcaggatggt ataaaagtga ctattccaga gggagctaca
    11161 tctaaagaaa ttatagcaat gttagtaagt aaaaatttag gagataaagc tacttttgaa
    11221 aatttaataa aaaaacctca agaattttat gataaatttc catatttgaa ggaagatgga
    11281 ataacctctt tagaagggtt cttgtatcca gaaacttatt attttaattc aaagaaacaa
    11341 tcagaagaag atattttaag cgaaatgtta aaagtatttg acagtaaata cacggacaag
    11401 tttaaaaaga aacaaaaaga attaaatatg actcttcaag aagtaatgga aatggcatca
    11461 ataattgaga aagaagctgt tcttgacaaa gacagaccta taatagcaag tgtgttttat
    11521 aatagactta aggtaggtat gcctcttcaa tcagatgcta caatacaata tatttttgaa
    11581 gagagaaaga aaattgtaac atatgatgat ttaaaaattg attcaccata taatagttat
    11641 aaaaataagg gattacctcc tacaccaata tcaaatcctg gtataaaatc tattgaagca
    11701 gcattatatc cagagaaaac agactattta tattttgtag ctaagattga cggtggaaat
    11761 aattatagta caaactatca agaccattta aaatacgtta aagaatataa agaagcaagg
    11821 gataaacaat caaaagatac aaaagcaaca aataaagaaa atacaaaaaa ataaaccatg
    11881 attataaaaa gtctaacagg gtaagaataa tgttgggctt ttttattgaa aattgttatt
    11941 cttagattta taataaagaa agtgtaaata tatataacaa tatgaattta aaaaataata
    12001 aattaatata aaacttgaat atttattatt ttagggagtg tttttatgag taatatagtt
    12061 aatgatttgg ttgaagatta tataagagtg acattaaaag aaaaagaagg atttttaaaa
    12121 gatttagaaa tttatgcaga ggaaaatagt gttcctataa tacataaaga agtttcagat
    12181 ttattaaaag tcttgttaaa ggttcaaaaa ccaaagagaa ttttagaagt aggatgtgca
    12241 attggttatt cttctatttt ttttgcaact attataggaa aagatgcaga tataataact
    12301 gttgagagaa atgaaaaaat gatagaaaaa gcaaaagaaa acataaaatt agctggattt
    12361 gacaataaca taactatact agaaggagat gcagaagaaa aacttagtca agtccaaggt
    12421 gaatttgacc ttatatttat agatgcagcc aaaggtcaat ataagctatt ttttgattta
    12481 gttatagata aattaaaaga tgggggactt ttagtatctg ataatatttt gtataaaggt
    12541 atggttgctc atgatgattt tgttgtaaga agaaaaaaga ctatagttaa aagaatgaga
    12601 aattatttag attatatatg taattgtgac tatttaagca catcattggt accaataggt
    12661 gatggtgtag cactaagtta taaagaaagt aaaagaggtg atgtagatgg aattaaataa
    12721 agtagaactt ttagctccag caggtgattt ggagagattg aaaatagcta taacatatgg
    12781 agcagatgcc gtttatatag gtggagaaat ttttggaatg aggtctgcag cgaagaattt
    12841 tagtaaggaa gatatggcag agggagtagc ttttgcacat gaaagaggca agaaagtatt
    12901 cgtaacagta aatataatac ctcataatga agatttttta caattagaag actatttgtt
    12961 ggaactagaa gagataggaa tagatgcagt tataatagct gacccaggag tgcttagtgt
    13021 cattaaaaaa gtaattccga atatggaaat acatttaagt acacaagcaa atacaaccaa
    13081 ttatctaagt gcaaacttct ggtacgagca tggaataaaa agagttgtag tggcaagaga
    13141 attatctttt gatgaaatat ctgagataag agcaaaaaca cctttagata tggatataga
    13201 agcgtttatg catggtgcta tgtgtatttc ttattctggc agatgtttaa taagcaacta
    13261 tatgactgga agagatgcaa acaaaggttc ttgtgctcaa tcttgtagat ggcaatatca
    13321 tttggtggaa gaaaaaagac ctggtgagta ttttccaata tacgaagatg aaaggggtac
    13381 attcttcttt aactcaaaag atttatgtat gatagagtat ataccagagt taatcaaatc
    13441 tggtataaca agtttaaaaa tagaaggtag aatgaagact gcatactatg tagctactgt
    13501 tgtaagagca tacagaatgg caatagatga attttacaga gacccagaaa actggaagtt
    13561 caatccaatg tggatggaag aactaaaaaa aggtagtcat agacatttta catcaggttt
    13621 ttacttgaat aaaccaacaa cagaagacca aaattatcaa tcagcatcat atgttagaaa
    13681 ctatgatttt ataggtatag ttagagaaac agaagatgaa gatggtctta ttgttgttga
    13741 gcaaagaaat aaaatgtgtg ttggtgatga aatagaagtt atgggaccat ataaagaaac
    13801 tatgtttact aaaattgaag aaatgtacaa tgaagaagga gaagctatag aatcagctcc
    13861 tcatcctaga caaatagtga agttaaagct atcagtaaaa gttggtaaag attatatgct
    13921 tagaaaagtt atagaagaaa aggttgaaga ataataaaaa attgataaaa atagtattag
    13981 ccccttagaa attgtcaata atgaaattat ctaaggggtg atttcatgtc aaaaaagaag
    14041 actccatttt tgaaaaaagt gggaaagaga agctggtgta tatttactat aattttaata
    14101 atttatagtg ttttaattta taggctagtt gatatacaag tattaaaagg tgataaatac
    14161 aaacaaagtg ttgaatcaca aagcgttgag aaagtcgagt taaatagtgg aagaggaata
    14221 atctatgata gaaacaacaa gaagcttaca gatacaagta aatctcaggt attaattgtt
    14281 gaaaaagaaa aattaaataa taactataaa attttggagc ttattaagaa agctactaag
    14341 atgaatgatt tagatatata taaagctgta caagagcaat tgacacgccc tataatacag
    14401 attcagacta aaaatatcga taagagtatg aaaaaagaac ttgagaaaaa tggaataatg
    14461 gtagaagaaa aaactatgag gtacgcaaaa gatggtttat tatcacatac aataggatat
    14521 ataaaagaag atgataaatc aggtcaatca ggaattgaaa agagtatgga tagtgtactt
    14581 agaaattcaa atgaaaagta tataagtgct tttaaagctg gagatgcagg aaatgaaaaa
    14641 tctctaaaca tactaaaggg aagtgtaaaa actgttgata ataaggataa agatagacat
    14701 ctaaaaacta ctattgatta taacattcaa aaaaaattag aacaaatact aaataaagaa
    14761 gaaaatccta cagcagcaat aatctcggaa gcatctacag gtgaaattct tgcaatgtgc
    14821 tctagaccta attttgacca aaatgacata tcaaaaagtt taaaaggaaa aaatggggaa
    14881 tttgaaaata gagttataaa agcgacatat ccaccagggt ctgtatttaa gatggtagtt
    14941 ttattttctg cacttgaaaa tggtgttata gatgagaact atacttacaa ctgtacaggt
    15001 aagactaaag ttgggaatac taatgaaata ctgcgatgta ataaaagaga tggtcatggt
    15061 ttccaaaact taagacaagc tttttctaat tcatgcaatc ctgcattttt agatatagct
    15121 atgaaacttg gaaaagaaaa gatactcaaa tctgctgaaa aattacattt atttgaaaag
    15181 gttgatattg gtcttgatga agaaaaaatt agagaagctc caaaaaacat atcaataaga
    15241 aatttagcta taggtcaaga aaatatagaa tttacaccac ttcaaataaa tcaaatgact
    15301 caaataatag ctaataatgg aacattcaaa ccactatact tatataaaag tcttgtggac
    15361 aataatatga atacaataaa aacttataag tcttcaaaaa aagaagaact tatttctcct
    15421 tatgtgtgta cacaagttaa agaatatatg aagtctgtat ctaggattgg tacagctaaa
    15481 gacctaaaag atattgaagg tggttgtgga gttaaaactg gtacagctca aagtagttta
    15541 aataaaaaag ctatagacca tggatggata acaggatttt atcctgaaga aagacctaag
    15601 tatgtaataa ctgtgttagt agaaggaact caaaaaggaa ataaatctgc aacacctata
    15661 tttaaggaaa tatgtgaaag tataaaataa attatgtact ataaaattta aggatatatc
    15721 gtaactttga ttttagatag aaaattttgt ctaatgatgt acgatatatc cttttaaaat
    15781 atagtattaa ctttcttgaa caagctcttt ttctagtttt ttaagagctt ttttttcgat
    15841 tctagataca tatgaccttg atatatcgag tttttgtgca atttctcttt gtgtcttata
    15901 gccataagga gttaacccat atcttaactg gacaatttct ctttctctag gagtaagaat
    15961 tttgtttagt tgttcgtaaa gttttccaac ttgaactttt agttcaactt catcaagaac
    16021 atagttagcc tctgtaccaa gtatatctaa aagacttatt tcattacctt ctttatctgt
    16081 tccgataggg tcttgtagag atacttgagt tttatttttt ttgttagatc taatattcat
    16141 taaaatctca ttttcaatac atctagaagc atatgttgct aatcgagttc ctttatctat
    16201 gttataagtt tctatagctt taattaatcc tattgtacct atggatatta aatcatcctg
    16261 atcttccgtt gaattattat atttctttgc tatataagct actaatctca tgtttctttc
    16321 aattaatgta tcttttgcac ttttatcgtt ttctatttta aatttagtta aatactcaat
    16381 ctcttcttca ggggttaagg gtttttcaaa agatttaaga gctgccaata ggtcaccccc
    16441 ttttcaacgg tcactttaaa ttatatgaag gagacttatc caagttgact gttatctaat
    16501 aaaataatat gcatattttt agaatttaaa acatatattt gcaaactttt gaatggaata
    16561 tgacataaag agtgggaatt taaatttaag tttttgttgt atatatatga aacaaggcga
    16621 agaaattatt gatttataaa gtataaattg gaattgaggt gtcaataaat ataaaaggaa
    16681 atagtattta tatatgtttt ggtgttgaag taaaagatta atgcaaagca ttagagtcta
    16741 taaaaatatg aatataggag gtgtaatttt acaatactta aaaaagtaaa tgtaattaat
    16801 tgtttgtata aagttgatta caagcaaaag tatcagtcat agttaatact gttaaggatg
    16861 aaggtggtgt gttaatttat atatgtatgg gaagaaattt tgattacaat gaaaaaactt
    16921 acaaaagata tttacgaaaa aacttacaaa agatatttac gaaaaaactt atagttaaat
    16981 gtttatatat aaaatattat ttaggggttg tttttattat tgaaaataag aaagagtggt
    17041 tttatatcaa ttgaatgtat aataagtatt gctatattat atgtggctgt ttatttagtt
    17101 tctacatcat tgtataattg ttatagtttt atcagtagaa atatatctga cagagaaatg
    17161 ttaagtacag caaaaaaata tatagaagat gagaagtata gaatacaaaa tagtaagtat
    17221 gagttaattg aagataagat agaaaaaaat tacataaatg gatatgaaat taacagtaga
    17281 atagagcaaa ttttagatta ttatcaatgc tatgaaataa atatagagat aaaaaatgaa
    17341 tttaaaaaac tgaggtttaa tagctatgtt actagaaaat aaaggctcta tattaataat
    17401 aacattgata atattttcta ttataagtac tgtgtgcatt atgtgtatag gtttaattta
    17461 tacacataat aatatgtttg agttaggata caaagacatt caactaaaag aaatttcttt
    17521 aagtggaata gaaatatcag taagtaatgt actttcttgt gttgaggaag ctattgatgt
    17581 atctaataat gaagacgaat ttagagaata tttcttagga aataatatga ttgaatgtat
    17641 aaaaaaaata aaagatacat cttatagtag tttagaagaa ctttctttaa aagtagaaaa
    17701 taatactatt tatgacagag gagatttttt tgaatttgat atagaaagta atgttagaga
    17761 aaaagaattt ttaaaagcaa taaaagtaag agtgaaaatc aaaaatccat ggataggaat
    17821 tgatattgaa gattatatgg ataaatctaa taacaataaa attgatgaca aaacattcaa
    17881 taattataaa gcatctgaaa ataatgaaat tataaatcaa tttaaaaatg aaagtgaaaa
    17941 tataaggaat caagaggttg ttgaatttac agaagaagat gaagaagaac ttaaaaaaaa
    18001 cataattgaa aatataaaga agaattttga tgaaaaaaac ctagtacagg tatataagta
    18061 taggggtgta taactttgaa aaaagaagga atgtgactgt aatagaatta atagtatcta
    18121 tcagtataat actcttgata gttacattat cttttccaaa agataatctt gaaaatcata
    18181 aaataaattt atttgcacgt caactttgtt cagatataag gtatgttagg cgaattaata
    18241 tgtttagcaa ctacaataca tatatatatt atataaatca aggtaaacac gaaggttatg
    18301 tattgagaaa agatggtaaa aacattaaat caataatgct acctaaaaat gcaaaaatag
    18361 ctaggagtat tgaaataata aagtttaaat ctgatgggtc gcctcaaaag gcgtctacaa
    18421 tagagatata taataataga ttaagaaaaa ctataacaat aacacctgta agtggaagag
    18481 tacttctgaa agagggaaaa tatgaaacat aaatatggtt acttgctttt ggaaagtgta
    18541 gtatcattgt cttcaatggt gataattata ctagttttat attctatatt tttgtctaca
    18601 ataaacttaa agttaaaagt agaagataag atagaactac aacaacaaag tcttgaaatc
    18661 ataaaatcta tggaaggaat tattagcaat tctatgggaa taatgaatgt atctaattat
    18721 gaagaaacat ttaaaaagac aacgtctata aaatgtagat atgtagatga aaataataat
    18781 gaagaaggca taagtaataa agaaattata ttgaatgaaa gacgaaataa gttatttgta
    18841 aattctttaa atggcgaaag tagtcaagca ggtggttatg aaattggaga ttatgtagat
    18901 gagatgtatg tatcaactac taataatggt caatatgtta atattaaatt gaaattatca
    18961 aaacgtagtc aaaaatatga gactgatttc aaaataaaag tatggaattt tagtgaaagt
    19021 atataaaaat acataaattt aagataaaga aatgacatca ataagtcata taaaatataa
    19081 ttattgagaa ctatatagct aaataatatc aatatgtaaa gtgtatagat taagaatata
    19141 gaggataata ataaaaatat agaataagta atttgccaaa tacttgttta tattgtataa
    19201 cttatgatat tatgatatta gtaaaaattt gtaatcctga cttgacagaa ttcaagtaag
    19261 agatataatt ttaaaggcat agtaatgtaa aatttactaa aaaataagaa atttatttta
    19321 atatggagga acaaaaaatg gtatcagcag gtgattttag aaaaggtgtt acatttgaaa
    19381 aagatggaca accatgttta gtagttgatt ttcaacacgt taagccaggt aaaggagctg
    19441 ctttcgttag aactaaatac aaaaacttaa aaacaggagc tataagagaa gaaagtttca
    19501 atccaagtga aaaattccct aaagctgtta tagatacaag acaaatgcaa tatctataca
    19561 atgatggtga gttatattat tttatggatc aagaaaattt tgaacaaata ccattaaact
    19621 atgagcaagt tgaagatgca attaagttct taaaagaaaa tgaagttgct acaataagat
    19681 tttaccaagg acaaccattc caagtggaag cgccaaactt tgcagagcta gaagttacag
    19741 atacagagcc aggtataaaa ggtgatacag caagtaatgt aactaaagca gctacagttg
    19801 aaactggagc agttgttcaa gttccattat ttataaatac tggagataaa gtaaagatag
    19861 atactagaac aggtgaatat ttatcaagag tataaaaatt tatgtatata aaagcttata
    19921 tgtcttaata atataagata tatgagcttt tagtttatat ataaaaaaga aagcgagggg
    19981 tgttacatga aacatttata tgaagaagtt gcatacttaa aaggcctagc agaaggacta
    20041 gaaataagtg ctgaaagtaa agaagggaaa atgatacata aaatagttga tgcattagaa
    20101 gtatttgcag atgctattgt aacattggat gaagaacaag aagaacttca agattttgtt
    20161 gaatctatag atgaagattt agcagattta gaagaagata ttgctgacat ggaagaagat
    20221 ctttatgaag aagatgacga tgaggatgat gaggacttca gctatataga aatggaatgc
    20281 ccaaattgtg gagagttagt agaaatagac gaagatttgt tatatgatga tgaagtagat
    20341 gttgtttgtc ctgattgtaa ggctgtgata ttatcatcag aagatgattg tgatgatgat
    20401 tgtacatgtg gtggttgttc aagttgtgat gatagagagt aaaaattcat aagaattaaa
    20461 actgcctatg atgcctatga gatattttgc ttcataggta gttttatttt tatgaggaag
    20521 ggtattatgt attttggttt tgcaatactt ggacaaaaat agtacactag aatttataga
    20581 ataggatttt ggagggatgt aaatatgaaa ataagtaaaa aattactaga taatatacaa
    20641 agatttgaaa atgttataaa ttataaattt aaaaataaag aatatatact agaagctctt
    20701 actcatagtt catattctaa tgaaaataaa aaatataatt ttaacgaaag attggaattt
    20761 ttaggggatt cggttttagg catagtgata agtgattatt tatttaatga agaagctaat
    20821 ctgcccgaag gggaactaac taaacttaga gcaaatatag tttgtgaaga ttcattaagt
    20881 gaagtagcta atgatataaa tttaggtatt cacatgttat taggtagagg agaagaagca
    20941 acaggaggaa gacataggac ttcaatatta gcagacgcat ttgaagctgt aatagctgct
    21001 atatatcttg atggaggttt tgaaagtgca agacagttta tacttcatca tatggaaaat
    21061 ataatttatg attcaagaaa aggaaatata tttagagact acaaaacgca tcttcaagag
    21121 gtgttacaag ggaatggaga aaataatatc tggtacaggc ttatagaaga aaaaggacca
    21181 gaccataata aaagatttgt aatggaagtt ggaattaatg acgatgtact tggtatagga
    21241 gaaggtaaga gtaaaaaaga agctgaacag ttagctgcta aaatagcttt aaagaaaaaa
    21301 ttatgggaaa aataaatata atatgtaaaa atataaaaag aaatatttat attagaagga
    21361 tgttgtagat gaaaaagaga attataccta tatttgttcc tcacaaagga tgtcctcatg
    21421 attgcatatt ttgtaatcaa aaaaaaataa caggtgtgag tactgatgtt actagtgaag
    21481 atgcaagaaa tataattgag gaatgtttag agactataga taaagatgca gatgtagaaa
    21541 tagcattttt tggtggtagc tttactgcta tagatattga tatacaaaaa agtttattat
    21601 cagttgctaa agaatatgta gaaaaaggtc ttgtaaaaga tattagaatg tctacaagac
    21661 cagattgtat tgatgaagat atattaagta tgttaaaaga atataagaca agtataattg
    21721 aactaggagt acaatcctta gatgaaaaag tattattaga tagtataaga ggtcatcaat
    21781 ctgaaattgt atacaagagt tcaaagatga taaaaaatag tggaatcaaa ctcggattac
    21841 agatgatgat tggtcttcca gctgacacag aagaaaagtg tatatttaca gcaaaaaaat
    21901 ttgtagagct aaaaccagat tgtgttagag tctatccaac tttggtagta aaagatacag
    21961 gtcttgaaaa gttaatggaa caaaataaat ataatccatt tactcttgaa gagagtgtaa
    22021 atatagtaaa aaaagttctt gtactgtttt atgttaataa tatcaatgta ataagagttg
    22081 ggcttcaagc tactgatgat atacaaattg gaaaagctgt tttagcagga ccatatcatc
    22141 ctgcatttag agagttggta gaagcagata tgataaaaga ttatttagaa tttgttattt
    22201 tgcaaaataa aaatataaag caaatgttgg tcaaatctaa taaaaagaat atttctaaaa
    22261 tcataggaaa taaaaaaaca aatgttaaat atatggaaga aaaatttggt gtgcttttaa
    22321 aaacacaaga atcagattta gatataaatc aattagaaat tgtgttagat gggaaaagtt
    22381 taattagtgc taatatgaga gacattcata gaaaattgta tgacatttat gacctttagt
    22441 tttaataatg aagggagaga aaagatttgt atttaaaaag actagagtta aagggattta
    22501 aatcctttcc agtaaaaaca gatattattt ttaaagaagg aataacagcc atagtagggc
    22561 caaatgggag tggaaaaagt aatatatctg atgctgtaag atgggtatta ggagaacaga
    22621 gtattaagag tcttagaggg gataaactcg aagatgtaat atttgcaggt acagatacaa
    22681 aaaaacctat gaattattgt gaagtggctc ttactattga taattctgaa aatcaattgg
    22741 aattagattt tacagaagta actataagaa gaagggctta tagaaatggt gaaagtgaat
    22801 tttttctaaa taataaaagt tgtagattaa aagatataaa agaagtcttt ttagatactg
    22861 gtattggaaa agatggttat tcaatcatag aacaaggaaa agtagatgaa atattaagta
    22921 ataatccttt aagcagaaga aaagtttttg atgaagcttg tggcatatca aaatatagat
    22981 ataaaaaaca ggaagcagaa aggaatttaa gtaatacaaa ggaaaattta gaaagaatag
    23041 atgatgtata tatagaaatt gaaaatcaat taaagcctct ttttaatcaa cagacaaagg
    23101 caaaaaagta cttggaaata agtgaaaaat taaagacact tgaagtaaat agctttataa
    23161 gagaaattga aggaatagaa aaagaattaa gtgaggttaa tgaacatcgt aaggttattg
    23221 aaaaggagct caatgaaaaa gaagaacaaa aaaatgttgt agagaaaaag caagaagata
    23281 ttaataaaga ggtagaggta ttacaagatg taattgaaaa atctgtagat tatataaatt
    23341 caattaaagg agttatatca aaaaaagaat cccaaataaa tttaattaag gaaagaataa
    23401 gaaattttac gaatgaaatt agtagaaaaa atttagaaat aaatgatata aaagagaagc
    23461 taaatgaaaa taagcaatac ataaaagaat tagaaagcaa taaattatca ggtagtgaag
    23521 aactatctac cttgcaggaa aatataaaag tattggaatc aagtaaagat aaacaaaaaa
    23581 ttaagttaga atccctaaat aatgagatag aattattgaa agaaagtata attgatatat
    23641 taaataagaa acaagagttt agtaataagc tgtccacttt aaacgcaaat aaagaaaata
    23701 tgaatatcag agatgaaaat ataaattcag agataacaga gttaaacaaa aatattgaaa
    23761 taaaatcatc tgaattagat actataaata aagaatttaa tatgcaaaat gaaaatttaa
    23821 agaatgtaaa taatagacat aaagaattat caattaattt gcaggattca attagtgaac
    23881 acaataaact agaagatgaa atacagaaaa gtaaatataa tttaaatgga tataattcaa
    23941 agttaaatgt ctatatagat atggaaaatc attatgaagg atttaataga ggagtaaaag
    24001 aagtactaaa aaataaaaat ttaaaaggag ttcatggagc attaggacag attataaatg
    24061 tacctgaaaa gtatgagaaa tctatagaag cagctcttgg tgcttatatg caaaatataa
    24121 taactgataa tgagtttagt gctaagtctg caataaacta tctaaaacag aataatttag
    24181 gtagagtaac atttcttcca ttaaatataa ttaaatcaaa taaaataagt ctaggaaatt
    24241 taaaagcaaa tactaagttc ataggaatag caagtgattt aattacattt gatgaaaagt
    24301 atagaaatat aattgaaaat atacttggaa gaacaatact tataaataat atagatgagg
    24361 gcataaaatt tgcaaaagaa actggtcata ggtttaaaat agtaacttta gatggtgaaa
    24421 tcttaaatcc aggaggttct ttgactgggg gaagtctaaa aactaatggg aatatactat
    24481 cgagaaaaag atacattaat gaatatacag aaaagataag taatatcaag aatgaaattt
    24541 cacatctaga attaaaaaga gaatctttag acaaagatgt gaaaaatatc aaaaatgaaa
    24601 ttgattcaca tgaaagtaaa ataaaagatt tagaaaaaag tataataata aaaagtacaa
    24661 gtataaagaa tgttgagtct gaaattgaat cgttaaaagg tagtattact aaattagaaa
    24721 atgagaagaa tgatttaaat tctaacctaa attatacact tgaaaaaagt gatgatgtta
    24781 gaaaaaatat ggaagagtta gatgatttat acaataaaaa taaagaaaaa attgatgcat
    24841 tgaatgagga aataaaaaga tacaatgatt tatatgataa agaaaaatct gaatttgatg
    24901 aattgaactt aagtttagtg aagaaaacag aagtttacaa tagtatagtt agagatataa
    24961 aaagaatatc tggtgaaaat tgtgagctag aagaaaaaaa taaacaatta gaagagtcat
    25021 taaattatga agaacatgaa ataattaaat tacaagattc tatactcact gaagaaaaag
    25081 aaaaagaaaa tttaacaaaa caactagggg atagtaatag aaatcttgaa actagaaaaa
    25141 tagcaaaaga tgatttaaaa aatagttttg atgaaattaa taaagaatta aaaactatag
    25201 atagacagca tatagaactt aaagaaagtt tatttaaagt tggtggaaga ttagaaagat
    25261 taaaaactag tcaagacaca tatataaata agttgtttga gcaatatgat atgaccttag
    25321 tacaagcatt ggaaattaaa gatgaagatt tggatataga tagaaaattc ttagaaagtc
    25381 taaagagaga aataagaagt cttggaaata taaatataga ctctataaag gaatatgagg
    25441 aaataaaaga aagatacgat ttttatagcg aacaaaagca agatttagaa gagtctatgg
    25501 aagagataga aaaacttata catactttag aagaaaatat gaaatctgaa tttgaaatta
    25561 agtttgaaga aataagtaaa aattttaaat atgtatataa gagattattt ggtggaggtt
    25621 gtggagaatt aactatttta gataaagaaa atttattaga aagtgatata ctaataacag
    25681 ctcaaccccc aggtaaaaaa atgaaaaatt taaacctatt atctggtgga gagaaagctt
    25741 taacggcaat aagtatatta tttgctatat tgattacaaa gccaacacca ttttgtatat
    25801 tagatgagat tgaagcgcca cttgatgacg caaatatatt tagatttgga gaatttttga
    25861 aggatttatc taaagagact caatttatat cagtaactca taggagagga actatggaag
    25921 ctgctgatta tatctacgga gttacaatgc aagaaaaagc catttcaaaa gttattagtt
    25981 taaaattaaa agaagctcag gaaataacag atattatata acttagattg acataggagg
    26041 aataacagtg ttaaaaaaat tatttggttt tggaaaagac aaggaaaagg aaatagaaaa
    26101 aaaagatgct gaagaagaaa tagaagttga agacagtgtt gataatttag aaaacttgga
    26161 agaaactatt ttctcaggac ttgaagaaga agtgatagat aaagtggaag atattgaaga
    26221 aaaaaatgag gaaagtgtca ataatgaagg cactgaagaa atcgaaaact ttgaaaaaat
    26281 agaagttgag atggatagta atggaaaaga agtagaaaat atttcaaata atgattctca
    26341 agaagagata atcgaagaat tagaaaatta tgatgaaatt gagtctaaag agatagaaga
    26401 taaagaagat aagaaagtta acttgtttga aagactaaaa caaggattga caaaagcaaa
    26461 gcaaggaata actgatagaa tagatgaagt tttaaaatca tatacaaaga tagatgaaga
    26521 attacttgaa gatttagagg aaattttaat aacagcagat gttggtgtaa atacaactat
    26581 ggatataatt gaaagactaa gagataaaat aaagcaaaaa ggtattactg aaccaataaa
    26641 ggtaagagaa gaacttaaat ctatagttga agatattctt acaaatgaaa actctacact
    26701 agatatagaa ccagcaccat gtataatatt aatggtgggt gtaaatgggg tcggtaagac
    26761 tactacaata ggtaaactag caaatagata caaaaaagat ggtaaaaaag ttttattagc
    26821 agcagcagat acatttagag cagcagcaac agagcaatta gaaatttggg caaatagaac
    26881 caatgtagac ataataaaac atcaagaggg agcagaccct ggagctgtag tatttgatgc
    26941 tataaaagct gcaaaagcta gaaaaacaga tgtattaata tgtgatacag caggaagatt
    27001 acacaataag gctaatctta tgaatgaact tggaaaagta tttaaaatag tagatagaga
    27061 atttccagaa gcaaaacgtg aagtacttct tgtggtggat gctacaacag gtcaaaatgc
    27121 agttgttcaa gcaaagacat ttaaagaagt tgcagatata acaggtatag tacttacaaa
    27181 acttgatgga actgcaaaag gtggagtagt ccttgcggta aaatcagaag tagatgtacc
    27241 tgtaaaactt attggagttg gagaaagtgt agaagaccta caagatttca atgcaaaatc
    27301 attttcagat gcattatttg gtaactaatg ttcttttaaa aataatggtt gacaagagtg
    27361 ttgttatgat ataaaataca atagtgtaaa gtaaataacc tttacagtat actgctagga
    27421 agtgtaagat atgaatatag aaaaaatggt cgaaattgga ttgttatttg aacagtataa
    27481 agaattatta actgacaagc agaaagaaat agttgctcta tactatgaag aagattattc
    27541 tttaggtgaa attagtgaaa atctgaatgt atcaagacaa ggtgtttatg ataccttaaa
    27601 gcgttcagaa aaaatactaa gagattatga agaaaagtta cacttagtat caaagattca
    27661 agagcaggaa aaaaatatta agattataaa agataaaatt attgacatta aagaagattt
    27721 attgcacaat agagattgtg ctaatttaat ccctaagcta gaaaatatag aagatgtatg
    27781 tagggagatg ataaaatgat atttgaagga ttgtctgata aactacaagg ggcatttagt
    27841 aagttaaaat ctaaaggaaa acttacagaa gtagatgtaa aaaatgctat gagagaagtt
    27901 aaacttgctc ttcttgaggc agatgttaac tttaaagtag ttaaagattt cataaaaaaa
    27961 gttcaagaga gatgtgttgg gcaagaagtt atgcaaagct taactccagc tcagcatgta
    28021 atcaaaattg taaatgaaga gcttacaagt ttaatgggag atgtacaaag taaagtaatg
    28081 atttcctcaa agcctcctac aattttaatg atggttggat tgcaaggtgc aggtaagact
    28141 actacagcag gaaaacttgg aggatatttt aaaaagcaag gtaaaaaacc tctattagta
    28201 gcttgtgata tttatagacc agcagctata aagcaacttc aagttgttgg agaaaaattg
    28261 gatatacctg tattcaatat gggagataaa gaaaatccaa taaatattgc gaaagcaggt
    28321 ttaagccatg cgattaaaaa tgctaatgat ttagttatta tagatacagc tggtagactt
    28381 catatagatg aagtacttat ggatgagtta aagtctatta aatcagaagt taagccacat
    28441 gagatacttt tggttgtaga ttctatgaca ggtcaagatg ctgtaaatgt tgcagaaagc
    28501 ttcaatgagg cacttggtgt tgatggagta gtattaacta aattggatgg agatactaga
    28561 ggtggtgctg cactttcaat aagagcagtt acacaaaagc ctataaaatt tatggggatg
    28621 ggagaaaagc tagatgatat agaacctttc cacccagata gaatggcatc aagaatatta
    28681 ggtatgggag atgtcttaag tttaatagaa aaagcacaag aaaatataga cttagagaaa
    28741 gctaaagaat tagagcaaaa aattaaaaaa caagagcttg attttgaaga tttcttacaa
    28801 caaatggaac aaatacaaaa tatgggacct cttgacaaga tacttggtat gatacctggt
    28861 atgggaaatg taaaagacca acttggagat gtagatttaa atggcaaaga aatgaagaga
    28921 acaaaagcca taattcagtc aatgactaca tatgagagaa gaaacccagg agtattaaat
    28981 gcttcaagaa agaaaagaat agctaaaggc agtggaacta gtgtacaaga tgtaaataga
    29041 cttataaaac aacttaatga aatgaaaaaa atgatgaaga tgtttgctgg ttcacaaaag
    29101 agtatgaaga aaaggggcgg cttaggcggt ttaccttttt ttaaataaaa tataaaaaag
    29161 taaaaattat tcggaggtga ctgtaatggc agttaaaata agattaaaaa gaatgggagc
    29221 taacaaaaaa cctttctaca gaatagtagt agctgattca agagctccaa gagatggaaa
    29281 atttatagaa gaaataggat actataatcc aatttctgaa cctaaacaag taagaataaa
    29341 tgatgaaaaa gctataaaat ggttagctac tggtgctcag ccaacagaag tagttaaaaa
    29401 attacttgta aaaaacggag taatagaaaa attcgaagct tctaaacaag caaaataatt
    29461 ttttcttggc aattagttta agtaagggct cattgctaag gttactttag gagatgaaat
    29521 atatgaaaga gctagttgta gatatagcta aggctctagt tgataatcct gactcagttg
    29581 ttgttgagga atttgaagat aatgatggta tcgtcttaaa gcttacagtt gctcaagagg
    29641 atatgggtaa ggttattggg aagcagggaa gaatagctaa agctataaga actgtcgtaa
    29701 gatctgttgc aaatagagaa aatataaaag tttctcttga gatagtataa aataggagga
    29761 ttaggtataa tgcctagtcc tttttatata aatgcgtagt ttaagatttt atgatgatat
    29821 gaccacataa gattaaattt tttaagaagg tgataacatg aaagaaaaat taactcattt
    29881 taaggtaggt caaatagtaa atacacaagg tttaaaaggt gaagtaagag tttatccgct
    29941 tactgatgat atagagaggt ttgatgaatt aaaagatttc tatctgggaa aagatttaaa
    30001 taataaattg gctgttgaaa aagtaagata caaaggttct atggttataa tgaagataaa
    30061 aggcatagac agcatagaga aagcagaaaa actaaaaaat aaatttatgt atgtagctag
    30121 agaagatagc agagagcttg aagaaggcga attttttata gcagatatga ttggaatgga
    30181 agttttaact gtagatggaa aatatgtagg gatactagaa gatgttcttc aatactcagc
    30241 aaatgatgtc tatgtagtaa aaggtgagga agataaagaa tttatgatac cagctataaa
    30301 aaaatttgta cctacaatag atattgatga aagaaaaatg attatagacc ctattaaggg
    30361 aatgatagat taggtggtaa tatgagattt catataatga cactttttcc agaaatattt
    30421 aattcgtaca tggatgaaag tataatgaag agagcagttg aaaaaggtat aattgaggtt
    30481 catatatata atataagaga tttttctaat aataaacata aaaaagtaga tgattatcca
    30541 tttggaggag gagcggggat ggtaatgact cctcaaccta tatatgacac atataagtat
    30601 ataataacaa ctcataatat aaataaccca agtgtaattt atcttactcc aaaaggtaag
    30661 gtatacaatc aatctatggc aaagcagatg tctttaaaag aagatataat actactatgt
    30721 ggtcattatg aaggaataga tgagagaatc attgatttaa tagttactga tgaaatttca
    30781 attggagatt atgtattaac tgggggagag ctcccagcac ttataatgat agattctata
    30841 tcgagattaa taccaggtgt attaaaccaa gaagaatcat ttgaagaaga atcatttaaa
    30901 gacaacttat tggaatatcc tcattacact agacctagag attttgaagg attaaaagta
    30961 ccagaagtgc ttttatcagg gaatcataag aagatagatg agtggagaag agaagaatct
    31021 ataaggataa ccaaagaaag acgatttgat ttatataaaa agtctaatga aaaataaaaa
    31081 tataatgaaa aattaatata tatgtataca ataaagtacc tataaatgga attaaaaaca
    31141 taaaagtgct tgtaataact attgaagtat agtataatat aaaatgttga aaatacgggc
    31201 gttcctctgt ttacactggt cgggtagtta agaacgtcta tgatatctag gaggaagaaa
    31261 gatgaatgaa atgctaagag caattgagca agaacaatta aaaaatgaag ttcctaattt
    31321 tggacctggg gacacagtta aagtacacgt tagaattata gaaggaaaaa gagaaagaat
    31381 acaagtattc gaaggtgtag tattaaagag acaaggtgga ggagcaagag aaactttcac
    31441 agttagaaaa atgtctttca atgttggtgt agaaagaact ttcccagttc actctccaaa
    31501 aatagaaaaa atagaagtta ctagaaaagg taaagtaaga agagctaaac taaactactt
    31561 aagaggtaga gtaggtaaag ctgctaagat aaaagaagct agaaataaat aattaaaaac
    31621 tttacttata aatggggggc tgatttatca gtcctctttc tattttaatg aaaattacag
    31681 caaataatta tactagaaat aaatagaaat atttgttgaa aatatacatt atttatctat
    31741 gaatttatag cttttaaaat gaaaaataaa attggtaaat attgcattat attttgtaac
    31801 aatattaaaa ataagagaac agtattatgt aaagtatgac gttaaaaaga tgacaatatt
    31861 atagtttgtt aagatagatt ttttaagtgg aattaggata taataatata atattaataa
    31921 ggagtgatac atgtgtcaaa tgaatacgaa gagtatctta tgaatgacaa tctacatata
    31981 aactggtatc caggtcatat gaaaaaaact aaggagttag taaaaaataa cttaaaattg
    32041 attgatgtag taattgaact tttggatgct agaatcccgt ttagtagtaa aaatccagac
    32101 atagacaggc ttgtaggaga taagccaaga gttgtcgtac ttaataagag tgatatggca
    32161 gatagggata aattgaatca atggattgaa tactataaga aaattaatat caaagctata
    32221 ccagtagata caataaaagg cgttggtata aataagataa tcgaagaatg taaaaatgta
    32281 actagagaaa aaatgagttc tttaaaagac aaagggagaa aagaaagagc tatacgaata
    32341 atgatagttg gagttccaaa cgttggtaaa tcgtctctta taaacaaatt aacaggtaga
    32401 aaaagcactc aaactggaga taagccagga gtaacgaaag ggaaacaatg ggttagatta
    32461 aagggaaatc ttgaactttt agatacacct ggaatacttt ggcctaagtt tgaagaccaa
    32521 gaagttgcat taaatttagc atttagtaga gctataaagg atgagatttt agatactgaa
    32581 actttggctt taagacttat agagaaactt atgaaaatag aacctgataa gttgaaagcc
    32641 agatataagt tagattgtct aggagaaact ccaattgaaa ctatggatat gattggccat
    32701 aaaagagggt ttataacagg taaaaaagaa ctagattata cacgcatagc cacaactgta
    32761 ttaaatgaat ttagagatgg aaaaatagga aatataacat tggaagttcc agaaaacgtt
    32821 aaaagataga ttttaaaaca aaatcaaaaa tttaatgtta aaaatttaaa aaagcctttt
    32881 agcaaaggct tttttatttt tttgaaatat agacaaatat ctccatataa aagtataaat
    32941 taggttagaa aacctttaaa aatattaatt taagacaaat atacctaata ttttgaaaat
    33001 taaaatgagt gtgattattt aaaattttag catattgact tgcttgtttc gtataatata
    33061 taataagcac aaataaatac aatatattaa aaatttaatt tatttaattt tattaattta
    33121 ataataagtt cttattgttt aattcaactt acttatgcaa gcgtaaacaa tcgagacatt
    33181 ttattaattt aataaggtta aaaaggggga ttttataatg ggtaagacca aggatgttat
    33241 agtatttgga tttgcgttat ttgcaatgtt ctttggagca ggtaacttaa tatttccacc
    33301 ttacttggga ataattacag gtccagaatg gttaattgcc ttcttaggat ttacgtttgc
    33361 tgatgctgga ttggcacttc tagcagtaat ggctacagct aaattcgatg gaaacgtagt
    33421 tgaaatgttt aaaagatgtg gtatgaagtt aggaattctt ataggttgtg cagatatttt
    33481 atgtataggc ccattcttag ctataccaag aacaggtgca acaacttatg aaatgggtat
    33541 aatgcctttg tttggaactt caattccagt gttattattc tgtatacttt tttttgcgat
    33601 ttcatatgta ctaacaataa gaccttctaa ggtcgtagat atagttggac agtttttaac
    33661 tccagctctt ctaatagctt tagcatttat aattataaaa ggtattatat cacctcttgg
    33721 agatatagta gataaaccta tgatacctaa tgtatttgca gaaggtatag gtcaaggata
    33781 tcagacaatg gatgcttttg cagcaatagc acttgcatca gtgctaatag tatctttaaa
    33841 tgataaagga tattctacta ttagtgacaa gttgaaaatg attggtaagg cgggtgttct
    33901 tgcttgtgga ggattagctc tagtttatgg aggattatgt ttccttgggg caactgtatc
    33961 aacaatgtat ggaacagatg cagtacaatc acaagtaata gtaaatataa ctgaaggatt
    34021 gcttggaaat gttgggaaag cgattttagc agtagtagta tcattagcat gtttaactac
    34081 atctataggt cttacatctg ccacaggtca atatttctct agacttacaa aaggtaaatt
    34141 gagttatgaa aaaatagtat tagctgtatc tgtattcagt gcagtagtag catcttttgg
    34201 tgttggaact ataataaaga tagcatctcc tatactaagt atagtttatc caccatcaat
    34261 tgttttaata attttagctt tcttcaatga aaaaattaag aatgataatg tatacaaagg
    34321 agcagtatat atgtcattgc ttgtaagtat actaacagtt atatctagtt atggagtagc
    34381 tgttccagta gtaaattctt tacctcttaa ttcattagga tttaactggg tggtgccagt
    34441 tataatagct ggaataattg gtaattttat accatcaaag agtcaatcta atacacttgg
    34501 aacaaactaa gttttgttta attaaaatat aatcaattgt aaataaatga gttaactaaa
    34561 agtaaaagga gttatttgta caaataactc cttttatttt tagattctat att
//
LOCUS       DAELJO010000019        34562 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELJO010000019 DAELJO010000000
VERSION     DAELJO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.019"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(108..755)
                     /locus_tag="KPB88_000839"
     CDS             complement(108..755)
                     /locus_tag="KPB88_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG0053141.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(784..2334)
                     /locus_tag="KPB88_000840"
     CDS             complement(784..2334)
                     /locus_tag="KPB88_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0053142.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(2537..3421)
                     /locus_tag="KPB88_000841"
     CDS             complement(2537..3421)
                     /locus_tag="KPB88_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0053143.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDESNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3649..4656
                     /locus_tag="KPB88_000842"
     CDS             3649..4656
                     /locus_tag="KPB88_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0053144.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            complement(4868..5566)
                     /gene="yunB"
                     /locus_tag="KPB88_000843"
     CDS             complement(4868..5566)
                     /gene="yunB"
                     /locus_tag="KPB88_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG0053145.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            5780..8473
                     /locus_tag="KPB88_000844"
     CDS             5780..8473
                     /locus_tag="KPB88_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0053146.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKDDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(8747..9682)
                     /locus_tag="KPB88_000845"
     CDS             complement(8747..9682)
                     /locus_tag="KPB88_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG0053147.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(9726..10898)
                     /locus_tag="KPB88_000846"
     CDS             complement(9726..10898)
                     /locus_tag="KPB88_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0053148.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(10885..11349)
                     /locus_tag="KPB88_000847"
     CDS             complement(10885..11349)
                     /locus_tag="KPB88_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053149.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(11364..12206)
                     /locus_tag="KPB88_000848"
     CDS             complement(11364..12206)
                     /locus_tag="KPB88_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0053150.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            complement(12581..13597)
                     /locus_tag="KPB88_000849"
     CDS             complement(12581..13597)
                     /locus_tag="KPB88_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0053151.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(13780..14142)
                     /gene="spoVAE"
                     /locus_tag="KPB88_000850"
     CDS             complement(13780..14142)
                     /gene="spoVAE"
                     /locus_tag="KPB88_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG0053152.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVIFTPKAKP"
     gene            complement(14156..15169)
                     /gene="spoVAD"
                     /locus_tag="KPB88_000851"
     CDS             complement(14156..15169)
                     /gene="spoVAD"
                     /locus_tag="KPB88_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG0053153.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(15336..15773)
                     /gene="spoVAC"
                     /locus_tag="KPB88_000852"
     CDS             complement(15336..15773)
                     /gene="spoVAC"
                     /locus_tag="KPB88_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG0053154.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            complement(16053..16817)
                     /gene="sigF"
                     /locus_tag="KPB88_000853"
     CDS             complement(16053..16817)
                     /gene="sigF"
                     /locus_tag="KPB88_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG0053155.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            complement(16840..17268)
                     /locus_tag="KPB88_000854"
     CDS             complement(16840..17268)
                     /locus_tag="KPB88_000854"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG0053156.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            complement(17282..17617)
                     /gene="spoIIAA"
                     /locus_tag="KPB88_000855"
     CDS             complement(17282..17617)
                     /gene="spoIIAA"
                     /locus_tag="KPB88_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG0053157.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            complement(17705..20059)
                     /locus_tag="KPB88_000856"
     CDS             complement(17705..20059)
                     /locus_tag="KPB88_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0053158.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMVEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYVSDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMTSNKVNCVSDELDMTSNKVNYVSDELDMT
                     SNKVNYVLDELDITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            complement(20408..21181)
                     /gene="srlD"
                     /locus_tag="KPB88_000857"
     CDS             complement(20408..21181)
                     /gene="srlD"
                     /locus_tag="KPB88_000857"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG0053159.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            complement(21282..21647)
                     /locus_tag="KPB88_000858"
     CDS             complement(21282..21647)
                     /locus_tag="KPB88_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0053160.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            complement(21688..22647)
                     /locus_tag="KPB88_000859"
                     /pseudo
     CDS             complement(21688..22647)
                     /locus_tag="KPB88_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            complement(22711..23271)
                     /gene="srlA"
                     /locus_tag="KPB88_000860"
     CDS             complement(22711..23271)
                     /gene="srlA"
                     /locus_tag="KPB88_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0053161.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            complement(23300..23734)
                     /locus_tag="KPB88_000861"
     CDS             complement(23300..23734)
                     /locus_tag="KPB88_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG0053162.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            complement(23739..25724)
                     /locus_tag="KPB88_000862"
     CDS             complement(23739..25724)
                     /locus_tag="KPB88_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0053163.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            complement(25999..27612)
                     /locus_tag="KPB88_000863"
     CDS             complement(25999..27612)
                     /locus_tag="KPB88_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0053164.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            complement(27703..28764)
                     /locus_tag="KPB88_000864"
     CDS             complement(27703..28764)
                     /locus_tag="KPB88_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG0053165.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            29057..31288
                     /gene="pflB"
                     /locus_tag="KPB88_000865"
     CDS             29057..31288
                     /gene="pflB"
                     /locus_tag="KPB88_000865"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG0053166.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            31448..32182
                     /gene="pflA"
                     /locus_tag="KPB88_000866"
     CDS             31448..32182
                     /gene="pflA"
                     /locus_tag="KPB88_000866"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG0053167.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(32304..33974)
                     /locus_tag="KPB88_000867"
     CDS             complement(32304..33974)
                     /locus_tag="KPB88_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG0053168.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT        11409 a         5934 c         4081 g        13138 t
ORIGIN      
        1 taactacttt catagtctaa atattattgc acagctattt ttattgtata acttttaatc
       61 taatcctttt ttattatctt attttggaaa taaaaatatc cctctattta acattgaaat
      121 atttttattg ataaagattt acataattta tacattacaa acagtaatat acttactaac
      181 aaaattaata ttaaaatcat cattccagag tgaaaaaaaa attcctctgg caatatattt
      241 ataactgggt ctaaatcggg attaaatatc caataatcat ttctaaataa taatttatga
      301 aaaataataa atgacttttc aaaatttaat aaaattggaa ctgttaataa taatggcata
      361 attataagtg ttattgatgt agtttttaag atttttatat ttttattttt tacactcaat
      421 ataattccta ctaaagaaac taccaataat aatttagcta atttatttat attttgaaat
      481 atattcctga cttcttcaaa atgtatcttc ccttgtggag aagattttaa tgttggtaat
      541 ttgaactcag acacattctt atttaagtta taatcaatta gataatcgta gttcaatttt
      601 atatcatctt tagataaacc agataactct gatatattta agtaatcaat gtcaaaataa
      661 taaagttgtt taaatccaac agtaaaatta ataacaccta ctattataac aatactaata
      721 caaattgaaa aaagtacatt tagaaacttt ttcatgaaat ccccccagca ataagttaaa
      781 tcactacacg attttagttg taaaatcttc tatattccat acatcatcaa caatttctga
      841 ataaaattct ggctcatgac atactaaaag aactgttccc ttaaactctt tgatagcctt
      901 cttcaattca tcttttgcct caacatctaa atgatttgta ggctcatcta atactaagaa
      961 atttatattt tttaacatta ctttgcaaag tctaacctta gcagcttctc caccacttaa
     1021 tactctcatt tgactcgtta tatgttcatt tgtaagtcca cattttgcaa gagcctgtct
     1081 aacttcaaag tttgtaagcc ctggaaattc actccaaact tcatccatag gtgtattact
     1141 attttctctt gaactctctt gttcaaaata tccaacttcc aaatagtcac ctaatttaac
     1201 atttccttca aatggtttta ttattccaag taaagttttt aatagggtag attttccaat
     1261 accattcatt ccttttaagg caatcttttt tcctctttct aaatggaaat taagtggttt
     1321 tgtcaatgct tcatcatacc ctaaaactaa attttctgtt tcaaaaatta ttttactagc
     1381 tgctctagca tctttaaaag caaaagtagg ttttattttt tcttttggtc tttctaatat
     1441 ttccatctta tcaagttgct tttgtctact gttagccatt cctctagttg ctactctagc
     1501 tttatttcta gctacaaaat cctctagtct ttttctttct tcaacttgtt tatcataagc
     1561 ttgttcttct tgacgtttct ttatatcatt aagtcttaca aattcatcat aatttccttt
     1621 atatctattt aactctccat tttccatatg atatattaca ttacatgtat tatttataaa
     1681 ttcaatatca tgagaaacta agacaaaact attctcatac tcttgaaggt acttagtaag
     1741 ccatgttata tgctcttcgt ctaaatagtt tgtaggctca tccaatataa gaatagttgg
     1801 attttcaagt agaagtttag taagaagtac ttttgttctt tgccctccac ttaagtcagt
     1861 tacatcttta tcaagaccaa tttctccaag accaagacca tttgcaactt cttgtatctt
     1921 tgcatctatc atataaaaac cactattttc aagtatagtt tgtatctctg ctgtttcttc
     1981 aagaagctta ctcatttcat catcactagc ttctcccatt ttgtcataca tagcaatcat
     2041 ttcttgctct aagtcaaaca tatgcttaaa agcctctcta agcacttctc ttatagtttt
     2101 tcccttgctt aataccgtat gttggtctaa gtaacctact gtagctcttg aagaccattt
     2161 tatatttcca gcatctggca taagcttctt tgttattata tttaaaaaac tcgattttcc
     2221 ttctccatta gcaccaacta gtgcaatatg ttcacctttt ctaagtctaa aggatacatt
     2281 ttctaatatt gttctcgcac caaatccatg actcacattt tctactacta gcatttcaac
     2341 acctctcaaa aaaattttac acattctata ctataatata ttctttttaa acttaaatca
     2401 agtattccca atatttttag ttatatttac gacaatttaa cctaaaataa acttcttctt
     2461 tgtgttatgt acaataaaaa aatagagata aaaattagga aaataatacc aacctaactt
     2521 tttctctatt catatcttat ttaaaatacg catccaatcc atcagctata cactgcatca
     2581 tttttctttg gtagtctgga tttgacatca tttggtcttc attatagtta ctcataaatc
     2641 ccatttctac taatacagct ggtacttttg accagttgaa tccagtcaaa tcgcctcttt
     2701 gaacgattcc attgatttgt atacctgatt gttccatatt ctgtttgata aattcagcac
     2761 atttattact ttcttcatat ataggagctg tatattttcc atctttttca ggaatcaata
     2821 tagatgctcc agtttttgaa gaattattta aactatctgc atgtattctt acaaccatat
     2881 ctgctttttt gtcattagca agtattgctc tttctgaatt acttatattt acatcatgtg
     2941 tctctctagt cattattaca ttataacctt tagattctaa tatactcttt aaaacaagag
     3001 atgcttctaa gtttaaaaca tattctggtt tttttgtagc aataccctct gttcctgaag
     3061 atactctcgc ttttttagag gaagaacctg gagctactgg ttctaaattg ctatctcctt
     3121 ttccttgatg ccctgggtct atacaaatta aaagtttttt atttttttga atttcatttt
     3181 tattatctga cgatttgtcc tctgatgact tatctttatt acttttagaa ttagattcgt
     3241 cttcattttt atctttatta tttttgtttg aattttcatc tttatctgct ttttcatttg
     3301 tgatatttcc tgaactaatc aaatttgtag tcttgtaaaa attgctaact ggatttgatg
     3361 ttcctataat tatgattccc atggcaatta tacaagtaac taacaatcta tattttttca
     3421 ttttttgctc ctttcttatg ctagacagtt attgtttatt tttatttata tgaatatgta
     3481 tttattttat catatacact atttatttta tttattttta tgaaaactaa ggtgttattg
     3541 agctagtttt ccaagcatca aaacatgatt cttgcacaac ataatataga agcagcaaga
     3601 aaacttcaat tctattatta aaataattta ataagaaaga ggtgttttat gcaacttaaa
     3661 aattttcatt ttaaaaataa tactttaagt aaaaaaacca acttagctaa gtcaaaattt
     3721 acaattgttt taacattttt atttttatta tattttttct caaataattt aatttatgca
     3781 caaaatttac aaaaaaatga acctgaatat ttaataccaa ttggaaacgt acttcaaatt
     3841 gacgcagaat taaaaaatat catagttaga aatcctggag aagcatctcc atttagatta
     3901 ggtgatgcta tagtaaaggt aaataatgct gaagttgatg gatatagtga ctttgccaat
     3961 accttaaatg ctttaccaga agaaaaacaa gtatctattt tattaaatag aggtggacaa
     4021 cttataacaa tgaaaactac caaacacata ttagaaaaaa ttagttttaa caatttactt
     4081 tctggatttg ctactttaac gtatatcaat ccagatacat cagagtttgg agctgtaggt
     4141 catccaataa gtgtgggtaa tgcaagaaaa atacctatta aaacaggttc aatttctaca
     4201 actaagcacc ttaatataga aaaatcttat aaaggtagtg ttggatgtat aaatgcaaaa
     4261 cgagaaaata ttattggtag ctttacagaa aatactgatt ttggaataag aggtcaaatt
     4321 aataactttg atatttccaa gttaaaaaaa tataaagtag catctttaga tgaagttaaa
     4381 ttaggaaaag cacaaataat tttacaagat aactttaata agtgtaaaaa atataatatt
     4441 gaaattatag atatagaaaa ccaaaaacat ccagaaagta aaagttttaa aattaaaatt
     4501 acagataaaa agcttttagc tcaaactggt ggtattgtac aaggtatgag tggtactcca
     4561 atagttcaag ataataaaat aatcggagca gtgtcacatg caattgaaaa cgacccagct
     4621 gtaggttatg gtgtatttat taaatggatg ttgtaaataa tataaataaa taagttttaa
     4681 gatgttatta agttattaat aatatatttg gtctaaaggt acttttattt aacctacaga
     4741 ccaaatatat tagatatttt acatagtata aaatataaat ttttattaaa attatattaa
     4801 tattatttcc aaaaatttta tttataaact attattttga atttatttaa aaatatacaa
     4861 ctttatatta cttgttggta ttctttccag tgttaggtaa aacatttcct agattttttt
     4921 ctggaatatt tacataactt tctggaacat cacctactat tatagtttca caaataggta
     4981 tttgagcttt aatttgtttt gttgaagtta taagtggtat tactacttta acttgtgttt
     5041 gagcttctaa atatattcta tgtctagttt gatttatacc tgaagactca aattccgttt
     5101 taaagtttac tgatactgtt cctataggtt caatagatac ttcaagttta ggaccgtatt
     5161 tagctaatat aggacttcct aatgctgttc ctataggtat gtaagaactt gctgtcttat
     5221 ccttttttaa ttcatcttgt atttctaaag ctattgcaga tgcaatttca ttcatcatta
     5281 tagtgtttgc ttgtatcata gttattttac cattattatc aagttgtata tccattaaat
     5341 cttcataatt tatttttcct tctaccatct cagcaacagc tttatttatc gacctatttg
     5401 ctagttcaag agctttagtc tctgctaaaa ctgttattgt cggtcttaaa gttttatcta
     5461 tatatataaa actgcctata aatacagaca agaaaagaat tactaaaaaa atagctatga
     5521 tttttctaaa ttcatttttt gtttttctat ctactattct attcaaaatt atcccctcct
     5581 atttatttat atgtagcttt ggcataattt ttataatatt tgtcaaaaat aaataaaata
     5641 aaaatataga ctttttaata tattacgttt atacatatac aatgtacaga atattatagc
     5701 aaattcattt tttctattaa ttacaaaact actcctattt gtgttataat attgcattaa
     5761 gtaaggaggt taaaatctta tgaataacga taatgataaa aatggaaata aaatcaggag
     5821 aaaaaaagtt agctcatctg gtaatacaaa taaaccaatt aatagaactt cagctaacaa
     5881 gactcgttca acaaaaaaca agaaaaaatc taaaaaaagc gataaattta aaaaactaag
     5941 agtatttggg atagtatttt tagttttact agttgttggt acagcaggta ctgctggtct
     6001 agtatttgcg tcactaagag atgtaactcc agttacagaa gctgttttag ataaacagac
     6061 gaaccaaaca actactatta aatacgctaa tggaaaaaca ttatctacag ctccaagtgt
     6121 aaataaaaag acacccgttc cattagataa gatttcccct tatcttcaac atgctgtaat
     6181 tgcaatagaa gatgagcgtt tttatgagca taagggtgtc gatattaaag gtttatttag
     6241 gtcagtatta aagaccctta caggtactaa gcaaggtggt agtactattc ctatgcaggt
     6301 atccaaaatg ctgttaacaa ctgaacagca aactatacct cgtaaaataa aggatattta
     6361 ctatgcacat gagatgagta aaacagtaag taaagataaa atactagaaa cttatttaaa
     6421 taacttcttc gttggtagag gtctagcagg tgctgaggct ggtgcacgtg gttattttga
     6481 taagtctgca gctgacctta ctctagcaga gtcagcactt cttgctggtt caactaaaaa
     6541 tccatcaaga ttttcagcct ataaaacttc taagttagag ggaaatgaaa ctaaggatga
     6601 tttagagaat aaattattat tttttgttaa cacaactgat gatgatttag atgaccctac
     6661 tcaagtggac tttgatatga ttgaaaaaat aaaatcttgg gagcttatat ctaatgatac
     6721 ttatagacaa ttaaaagcag gtacattagt tgttagaaag gctgtaagta atcctgaagc
     6781 taaaaaaaga caagaaattg ttttaaagaa aatgttagaa ttgaaatata taaaacaaag
     6841 tgaatatgat gaagcaatca aagctaaaat agaaataaaa ttgcctcaat catcagataa
     6901 agtttcttca tctgtagaag accttatcga aagtgaagtt ataaatgctt taatggaaca
     6961 aggacataca aatgatgaag ctcaaaactt attctacaat ggtggtctaa ttgtaaacac
     7021 aactatagac cctaaaatgc aagatgcttt agaagaagaa tttgatagaa atagcaattt
     7081 cccaggacat atggttggtc ctgatggagt tagccaaccg caagctgcaa tggtaatctt
     7141 agattataaa aatggtgaaa taagagcact agctggagga agaaatataa gtggtagaaa
     7201 aactttaaat cgtgctacta accctcatca gccaggttct tctataaaac ctttagcaat
     7261 atatacgcca gctattgata ctctaaaaat aacacaagca actgctctaa gtgattctcg
     7321 aggaggatat aagtttgaag aaaataataa atggaatcca agaactacta ctgctggtca
     7381 tggttctatg agtttacgta aagcacttgc taaatcttca aacacgatag ctgtcaaaac
     7441 tgctgaaatg cttggagact cttatgatga atgtgtagat ataatgatgg attacttaaa
     7501 gaactttggt ataacaactt taaagaataa tcactcaggt tcatctgaag caagtgatag
     7561 aaaattcccg tcattgacac taggtggtat ggcgaatggt attactccac ttcaaatggc
     7621 tgctgcttat ggtacattgg caaatcacgg tatttatgta gaaccttcta tttttactac
     7681 tataactacc tttgatggtc aattattagt taaaaatgct cctgaggaac ataaagttgt
     7741 agaacctgaa gtagcttatg tcgttacaga tatgttagaa agtgtaatta ctgaaggtac
     7801 tggtggtgtt gctgcacttc caaagggaat gcctgtagcc ggtaaaacag gtactacaaa
     7861 tagtgcatat gatgcttggt ttgtcggtta cactccttat tatgttggtg caacttatat
     7921 tggagatgat gctggtagaa aagatgattc tggtaacact ataaaacgta gagaagtacc
     7981 acatggaagt actagtactg caaaactatg ggaaaaaatt atggaaaaaa tacatgctaa
     8041 tttaacggtt actgaatttg aagttcctaa aaatgtatat tttacaaaaa ttaatttaga
     8101 ggatggagga aagcaatctt ctggctctaa agcagcattt attgaaggta ctgctcctac
     8161 taaagtaagt tctcaaccat catctgaaga tacaaaaaaa ccagacaata accaaccaga
     8221 agaaaataat aataacaata ataacacagg tgataatggt ggcggttcta cacctcctga
     8281 taatggcgga aacaatggtg gtggctctac tactcctcct gataatggcg gaaataatgg
     8341 tggtggctct actacacctc ctgataatgg cggaaacaat ggtggtggct ctactactcc
     8401 tcctgataat ggtggaaata atggtggtgg ctctactact cctcctgatg gtggtggaac
     8461 tccagcaact taataaaaaa tagaatttaa ataaaaatat aaggagttgt ctcaaggtga
     8521 ttaaaaaaat cactttgaaa cgctcctttt tttataatta caatatatta tttgtattca
     8581 taaaaaagag aatcccaata accagaatcc tcctttttat ataaacaaat aaatatttta
     8641 tttttaaagt ttttaactaa gtgctttatt cttactttta ttcctaattg tttatatatt
     8701 cttaaatagt tatatcacaa attatataaa cttaatattt ttctaattac tgtataccaa
     8761 atgtttgaaa tctatttaat ttgccatatc tcttaataag catttcccac tcttctttat
     8821 catagaaatc acacttattt ctaccaaata gcttggcaac ttcaattgca aatctcccag
     8881 ctgaatctaa atcactaaaa ttagttgcac ctgtagcaca tccagcaaca ggcatctctg
     8941 ttgtaattgc tactcctact attggtgcat ctgtagctac tgctggttgt aaaatactat
     9001 ttaagtgata cagattattt ccatatggtg ttatatcttg aatagacaaa gggaatacaa
     9061 atggagattt ccctgttaca gtctgcataa tatctaaaag gttatcacta gtttttaata
     9121 tgtagccttc tttaacagtt ggcgatattg caaatcctct agtattgatt actctatttc
     9181 ctttagtagt atctatagaa agtattgcat cacaactact atcaacttca ttttcattca
     9241 tagtcatcat atcaattgga gaatccataa aaggagtagg atagtgttct ttagttggag
     9301 catctgggca aatatgagtt gaaattacaa catcaccatc taatatatcc ccttttcgtt
     9361 gcatatccaa aagtttagca gccactgcaa ttgctaccaa tgctccatca ccatctgaaa
     9421 caaaacctat aacttctggt cttgctccaa tccctcctag tctacctaaa ataccaagag
     9481 taggagcatc tccacctttt aattttccat tttttccagg aattgtaagc tttactaaat
     9541 ctgttgaacc tttacttgaa gacaattctt ttacagttac atctacttca ccataacctt
     9601 gtaagtaagt ttttacttct tccccattag catttgcttt atctaaaatg tcataaacct
     9661 ctattacttg ttttaatatc atgtctaatc cgatttcctt tcttaatata ttttaaaatt
     9721 atatattatt tactatacaa aatatctact ccacacattg ctaacacctt aattgctttt
     9781 ataagtgcta ctctgccttc ttcaccacca gctgcaacag caaattcatc tgtatgagtt
     9841 gcagtatttt cttttaattt tatatatgct tgtattgcag gtatctcatg agttaaattt
     9901 cctgtatcag ttgaacctat accttgactt aaatctcttt caacatagtc ttcacccaaa
     9961 acttcaaaat tttgtctaac tatattcact aattcagaat cattcttaat ctctttgtat
    10021 atttcatcta attgaactat ttctacatta gttccagtag aaattgcagc tcctttagca
    10081 caatttttaa gcatttcaca aatctcattt aaatattcta tacttaaagc tcttacatta
    10141 aatatagctg tagatttttc aggaattata ttatgtatgc tacctccatc agttataatt
    10201 ccatgtactc ttgcataatc tttaagatgt aatctcatgc tatttacact gttaaatagt
    10261 gctattactc cacttaaggc acttttccct tcccaaggaa aagcagcagc atgtgatgcc
    10321 ttaccagtaa aatcaaattt taaattaact tgagcaaatg atatatcatc aggcattgaa
    10381 gcatcacttg gatgtaaaat taatgcagca tctacattat taaatacacc ttctttaatc
    10441 atagttattt ttccacctac tctttcttct gcaggtgttc caaaaacctt tacagtccct
    10501 tctatattat gttttttcat gatttctttt aatatgatac ctgcaccaac agaacttgtc
    10561 cctatgatat tgtgcccaca tgcatgtccc attcctggaa gtgcatcata ttcagctaaa
    10621 aaagcaactg ttttcccatt agttccattt gaatacacag cttcaaaaga tgtttctaat
    10681 cctcctacac ctttagttac attaaattca ttttcttcta acaaatcaca taatgctttt
    10741 tgtgacttat actcctcaaa tgctaattct ggatttgaaa ataaatagtc agaaactttt
    10801 tcaaatgatg tctttttctc ttcagataat aactttattt cctctttaat ttttgctttt
    10861 aattcattca tatttactac ctccttaaag cattccattt aatcttaaaa ctgtttgaga
    10921 tataaaagca gaaccaaagt aagtagatgt cataactata aatgatataa ctactaactt
    10981 ccaactcaaa gtctttaata tatctaattg gttacctaca gagattcctg caaaagctaa
    11041 taatggtgtt actgtagaca taaaattgat tttccctaca tttgttgcta tgatatcccc
    11101 cactggagat attggaagcg ataaaacgaa tgcaactaat gtagcccatg caaaacctgg
    11161 taaattaggt tttttaacaa aattctttat tataatagca attatcacta acaaaaatcc
    11221 agatacactt ccaatcaaca aatcttgagc ctgagtcttg agtgctatca tttgtccaaa
    11281 gcaaataata atatatataa acaatactat ttttatctgc tccataatag tcttaaaagt
    11341 tttttccata tatatatcct cctttacaat ttattacttg ttcttcttgt ttttcttcct
    11401 ctaaatcttt caataactgg agctactttg ctataatagt agttggctac tggtaaagat
    11461 aagaataatt ctatatatat tccagtaaca gctgtaatca atccactagt agctgaatat
    11521 gctaaaatct gctctgacat ttgtggaacc gtattcatta atgatgctgt tgcagctccc
    11581 atcattgaac cactacccat tcctgatgcc attgctaaag catatggatg aaatcctaaa
    11641 agtacaccca ctggtgctaa aaatgaaaat aataatgttc caaatatact accacttatg
    11701 taagttccta gtgttcctat accttctggt gagtttaatc catacttatc tccaataatt
    11761 cctagtgctg tgtcacgaca taatgaaaaa gtaccaccta atgcacttct tccaagaccc
    11821 aatgctactc caacaggtaa tgctatcaac attgtaccca tatgaccaaa ttcttgtaat
    11881 aaaagtgctg gtcctgcttg aagtaatata ccaactttag gccctgcatt aattccaaac
    11941 attatcccta atggatatag tgtcaaagat aataaatatg gagatacatt tatttcatta
    12001 tcccctataa acttttttaa ggctggtata actctcccta ataaatgagg agaaacagcc
    12061 attcctatga atatagccca tagtatacta agtaaagtta cattaattcc cataacttta
    12121 aatgaaatag gtccaattaa ttctgccaat attgtgatta taaaggctgc tattatggca
    12181 tttctaatcc ctgctttttc tttcatttct atacctcctt gtaattttta taattttctt
    12241 ataatataat tgttagtgat tttattatat aacctatcat tgataatgta taatatatat
    12301 ttattcatca tttttatata cttttagtta tgaatcaaaa aaatattata gattcatgat
    12361 ataaaataca aataatttat taatttatat tgttaaaata acggattttc aaataattga
    12421 ataaaattat tcattcatat tataaaaact atctcaataa ataaaggagt acaatgtact
    12481 tatatacacc tactcctttt aatttttatt tatattattt gttaaaaaac ttcacataat
    12541 ctataatatt tttaataaac gtttgatact taaaagctta ctactttatt cttttaacgc
    12601 tatttcgtat tattaacctt ggatataatt ttctcggaat gtgttctgta ctctttttat
    12661 ttattatatt tactaaacta tctactccat tttttataac ttgctctctt gggtcatcta
    12721 ttgttgataa atgcgaccta aaatatgata ttaattcaat attattatac cctattatgg
    12781 aaatatcttc tggtacattt ataccataag tagtttttaa ctcttgtatt atagaaatcg
    12841 ctggtacatc ttgttgtaca aatatagctg aagctttttt tattttatca atgtattttt
    12901 ctaaaaattc aacttgagat tcaaaatcca ttttagattt aatgacttct gtctttaaat
    12961 ttgcttcttc catagcattc aaatatccct tggttctatc ttcatgcatt tttgaactct
    13021 tatcatctga agttactgtt attatatctt tatgaccatg gtcaattaaa tgcttggtag
    13081 ctatataacc tccatacacg ttatcgtccc aaaagaaatt atctacttct cctataaaag
    13141 atggtttaaa atatataaat acatgtggaa aattattgtc ttttaaaaaa tcatactctt
    13201 ctttttttat tgattgagac acaattacta atccgtcaac catttgtcca tttaccattt
    13261 tatatacatt actatcacca ctaatgttat tattaggctg tattataaaa tcgtatttat
    13321 tagtctcaat agaatttata gaactatcca ttatatcact aaaaaacttt ctagtatctt
    13381 gatgattaaa attatttgaa aagattaccc ctatcctgtt agtttctttt tttgctaaat
    13441 ttcttgcatt tacattaaaa tggaacccta gtctatttgc ctcttctacc actttctttt
    13501 ttgttttttc agatacatta ggattattgt ttaagcacct agaaacagtt gaataactga
    13561 tacctagatt ccttgcaata tctttaatag ttaccatctt tatcccctac ttcacttcat
    13621 tttattttga taatataata ctacactatg ttgtaaataa ttgtcaactt caattatgta
    13681 aacgcttctt tatataaaaa acaggtggaa tctaaaagat atccacctgc tttataaaaa
    13741 gttattattt caaaaactta ttattctttt aataaagatt tatggttttg cttttggagt
    13801 aaatattact gacataatat atccaaagaa tatagctgct gctattccac cagctgtagc
    13861 agttgtacct cctaaaaaaa tacctaaaaa accatcactt tgaattgatt ttattactcc
    13921 tgtagcaagt gaatatccaa atcctattat aggaactgtt gcacctgctg ctgcaaactt
    13981 aactaatggc tcataaagtc ctagaaaagt taaaactaca ccagttgtta catacgttac
    14041 cataacatat ggagtttgca ttttgaaata atccatcatt acttgggcta ttaaacatat
    14101 aattccacct acaataaata ctcttacata gtccattaac atattatatc acttcctact
    14161 catttactat tactactgca tgtgcaacac atggtattgt ctgcttttgt agtgtacttg
    14221 ttggtgaaag tagtgctcct gttgatacaa gcattacttt gttaaattct ttttttctca
    14281 acttatcata tatatatcct gcaaacactg tagctgaaca cccacatcca cttccaccac
    14341 agtggacatc ttgttcttta aggttaaaca tctctatacc acagtcagtg taaacttttg
    14401 atatatctac acctttttct tttagaaagt cttctgctat ctgtttgcca agagtaccta
    14461 aatctccagt agcaattata tcaaagctat ttgggtcatc attagtatct tcaaagtaag
    14521 aatagatagt atctattgct gctggagcca tagctggtcc catattatta ccatcatcaa
    14581 taccctcatc tattacttta ccaacagtaa catactttac ttttggaccg tctccattag
    14641 gagctaataa tacacttcct gctcctgtaa ctgtccactg ggctgtcatt ggtttttgat
    14701 ttccaagttc aagtgggaat ctaaattgtc tttctgctgt acaatagtga cttgatgtac
    14761 ctgacagtac taaatcagca aaaccaccat ctataagcat agaacctata caaagacctt
    14821 ctgccattgt agaacaagct ccatatatac ctaaaaatgg aatagccagt tctcttgctg
    14881 caaatgatgc tggcactatt tgatttatta aatcgccacc tagcatataa tttacatctg
    14941 acaatttttt acctgccttt tggatagctt gttgagatgc agtctctacc attttacttt
    15001 cagctaattc ccaagtcttt tcaccatata aatcgtcagt cataatcata tcaaaatagt
    15061 cctttagtgg tccttcacct tccttaggac ctactatagt tccagttgat attatggttg
    15121 gtttattttc taacttgact gttctttttc caattctttt atttttcata ttttcacacc
    15181 tttttaattt tgcattttac tattcaaatt tttaatatta attaaaaata ttaattaaat
    15241 ctgcacaatt atttttcaat tagttacttt agtagctatt ttcagatttt ataccttctt
    15301 cataattaat aaaatttaaa ttaaataaaa atcatctaaa acatcttaaa aatataataa
    15361 ataattccac ataaaataga agaacctatc ccataaacta aaactggtcc tgcaatttta
    15421 aataagtttg cacctacacc taatacatag ccttctcttt tatactccat tgctggagat
    15481 actattgagt tagcaaaacc tgttattgga attatagaac cagctccagc tctttttcct
    15541 attaggtcgt aaactcctaa tccagttaaa aatgccccta taaatattaa tgttattgat
    15601 gttgcagaac tagcacctag tttatcaaga cctccaactt tcatatacaa atcatttatt
    15661 gcttgcccta tcatacaaat aattccacct acaataaatg caagtgtata gttttttaaa
    15721 tatgtaggtt ttgggcttat ttggtctaca tattttttat aatttttatc catactaaac
    15781 acctctctaa agtattattt gttaatttta aaaaaataaa cctattattt attttgacta
    15841 tctaaagttt ggtcaaaaaa taaaaaagat gtctaataaa gaaaaaatct atttttccat
    15901 aaagacacct tgtttttata tttttacttt tttaatgctt tttattattt taatatcttt
    15961 taataattta aaaacttaac ataaaaagtt attacatttt attttagtaa tatatttatt
    16021 atattttgtt attctacata tcataagtat tatcacgata tatattcttt taacttagac
    16081 aatactttct tctctatcct agatacttga acttgtgata tattgagcat ctctcctatt
    16141 tcactttgag tcatgtcttc aaagtatctg agaactatta tttgacgctc tctcttatcc
    16201 agtcttccaa gtatgtcttt taacaatatg ttatccacta ctttttcttc ttcactttct
    16261 cctttcatgc ttattttatc aataaggcat attggagaac cttcctcttc atgaataact
    16321 ccttgcaaat actcaacatt gaaattagct tccatagcca ttactaaatc ttctttttct
    16381 acatctattg ctttagctaa ttcttctata ctcggttctc tacctaattt tttagttaaa
    16441 gcttcacttt ccatctttgc ctttacagct atttgcttta acgaccttga tacttttatc
    16501 ataccatcat ctcttaagta tctctttatt tcacctaaaa tcattggtac tgcataagta
    16561 gagaatttta cattaaattt tgggtcaaat ttatatattg attttattaa gcctatagaa
    16621 cctaactgaa ataaatcttc tctatcatat cctatattta aaaatctaga tacaacgctc
    16681 ctaactagac ctaaattgct tgatattaat atttcttttg cctcttcatc tccattttga
    16741 actttttcaa taagttctag agtctcttca tgactaagaa gaggtttttt ttcttctctg
    16801 gcaacagtta cttccataat aaaacctcac ttttggactt tatttaggca catttatttt
    16861 tttagtcatt tcaattctag tcccattatc ttttacagaa cttacctcta catcgtccat
    16921 aaagcttttc attactgtga atcccattcc tgatctatca agttctggtt ttgatgtata
    16981 aagtggttcc attgccttct ccacattttc tattccatac ccttcatctt caactataat
    17041 ctttatagta tttccttcta tctcacatct taagaaaaca aacttagaac tgtcttcttc
    17101 atatccatgt attatagcat tcgtaacagc ctcagagaca gcagttttta tatctactaa
    17161 ctcatctact gttgggtcta acttagctgc aaaggatgct actataactc ttgctaaact
    17221 ttcattttca gatattgccg aaaatttaac ttccataata ttattcatca catactcctc
    17281 cctacaaaga acttagagct tcttcatacg tatcataaat gccaataatt ttatttaacc
    17341 cagataaatc aaaaatcttc ttaactcgtg aacttatatt aattactgaa actcgtcccc
    17401 cttcattaga aatcttttta tatctaccta tgatgacgcc tatacctgaa gaatccataa
    17461 aattaatatt ttcaaaatta aatacaacat tttttacttg atttacactt aataagttat
    17521 ctatttcttc ccttatttca ttggctacat ggtggtctag ttctgagcac ataaattcta
    17581 tgtatagatt cttatgttct aaggaataat ttaccatcaa aaatccctcc ttcaatagtt
    17641 ttgtaaaaat agtaactttt aatcaattct ataattatat ttttattcct tcaatgaaat
    17701 ttaactagtt ttgtcgtatt atttctacca tactttcatt atatgttacc tgacccccaa
    17761 aatctgatat accagtgtct gttaaactat taggatttcc atgtttcttc caccaaccaa
    17821 catacatttt tactatataa ttacctactc catcatctat atttatttgt acatttattt
    17881 ttgctttttt tgacctcaat atcacaatat ctttgtcctc aatacctact tttttagcca
    17941 ttctccgatt tatatatgct tgagctatag attttttgtc cataaaatgc tgactaaata
    18001 aagtatctgc atgatgatta gttaataatc taaaagaaat atttttatct tctatttctt
    18061 tatatttatt gcttatatat acaggtgttg gacttattcc caagtttttt atactttctg
    18121 aataaagctc gtattttcca gatggagtct caaacttctt atcttcccaa gcaacagggt
    18181 tatgtatagt aaagtaattg tttttaagtt tttctatatc cagatttttg tcaaaacgct
    18241 taagtggttc aatcactttt tctaaataag ttctcttttc tacaaaagga taatcattta
    18301 atcccatctt tttagctagc tccataaaga agtaatattc atccatcaat ttatgtgctg
    18361 gctttacagc tctttcatta taggttatat atggattggt cattgagcta taaattatat
    18421 cttcactctc cagtgtattt gtacacggta taaacatgtc acataaagta gctgtatcag
    18481 ttataaacat atcaaaacat actttaaatt caatttttga aaacaccctc tccaattcta
    18541 ctaaatctgg cagttgattc agcatattac ttttagttat cacagccatc tttataggaa
    18601 tattatcttt gattgactta ttagataaat tatacaattc atttgatata taatctgttt
    18661 tatttgatgt tatatcaagt tcatctaata cataatttac tttatttgat gtcatatcaa
    18721 gttcatctga tacataattt actttatttg atgtcatatc aagttcatct gatacacaat
    18781 ttactttatt tgatgtcata tcaagttcat ctgatacaca atttacttta tgtaatgcca
    18841 tatcaagttc atctgataca taatttactt tatttgatgt tgtatcaagt tcatctgaca
    18901 cataatttac tttatgtaat gccatatcaa gttcatctga tgcataattt actttatttg
    18961 atgtattttt taaagattct tctataaatt tactgatatt acttacataa aactccctat
    19021 cttcaccata tgattggcta ttgtaagggt cagaatttat aacactcgga taaactttat
    19081 tagcatagtt tacccctcca ccactaaatc ctatttgacc agttatagca cctaaagcat
    19141 ctattgctct tattgtgttt ccaccatttt tatatttttg aagcccatat ccaacattta
    19201 tacttgaata tttattagta tataaatcca ctaactctct tatatcattc tctttaacac
    19261 cacattcatc aattaaaaaa cttaattcca atgaagataa atatttttta tattcttcaa
    19321 aaccaataac atacgagttt atatattctt catcatataa tttatcttct actataattt
    19381 ttgccatagc tatagctaaa gctccatctg ttccaggatt tacttgtaca tacttatctg
    19441 ctatttgagc tgtttttgta tatatggggt caatcactac tatctttatc cctttttctt
    19501 tagctttttt aattatttgc atagtatgaa ttgtagtata tgctggattt tttccccaca
    19561 caaatatact tttactattt aacatatctt ctatagcatg actcttagct actccaaaat
    19621 catatttttg agcatgcatc cctgcactcc aacagggacc acccttatgt ctgctaacac
    19681 ctccataaaa attaaaaaat atatctccta tgtattttag cacagaccca cttccatatt
    19741 ggtcataatg catgacagac tttgatgtat acttttcttt atagtatgta agcttttcta
    19801 ccattatttc tatagcttta tcaaaagaaa tctctttcca tactccatca actttaagca
    19861 taggagtctt tattctatct ttatgattta atctatctaa atgggccatc ccttttttgc
    19921 atataaaacc ttttgtataa ggatgatttt tatctccttc aatttttact acattatttc
    19981 ctttagtata aacattgaat ttacaacagt catgacaatc taatgtacac ccatgactta
    20041 atttttttat tatatccata aaatatcctt ctttctactt tcttacctta ttaaaaatta
    20101 acatatatac tataatttat taaactttct atgttcaaaa ctacagtata taaaacatag
    20161 ctatatttta tcaaaataag tttaaaaata cttcataaat ataatattat taaaaaattc
    20221 tcttttaaaa aactaacaat cttatatgtt tacttaaaca ctatgttttt taattacaaa
    20281 aatgaggcta tctcaaaata aagataaacc tctttttcat tgtttaaatg ctgaaaatat
    20341 atttaatttt atacaaaaaa gtgcctcatg aactaaagag ttcatcttga gacactccat
    20401 aaaaacctta cataacttgt ccaccagtaa tatttataga ttgaccagtg cagtattcag
    20461 cttcctttga agcataaaat gtaagcatat ttgcaacatc tataaatcta caccctcttt
    20521 ttaaaggtac tttatctata taaacttgct tgacttctga ttcaggtatt cccaatttta
    20581 ctgcatattg aggaattaaa ctttcaaaca tatcagaatc aagtaaatta ccgagcatca
    20641 gtgcatttac agttatctta tgttctgcca aatctagcgc taaactttga gtaagtccta
    20701 ctccagcaaa tttacctgct gaatatcctg aattatgctt gcttcccact ctacctgatt
    20761 ttgaatttat ctgtattatt ctaccctcaa taccttttgc tatcatgact ttagatactt
    20821 ctcttgcaaa tagaaagtac ccatttaaat ttacgtctac aataaattta aaatctttgt
    20881 atccaaaatc agttattttt ttacttacag ccactcctgc attgtacact aataaatcta
    20941 ttctatcaaa atcattttca atagctttga cagtacttat aacctgtctt tcatctatgg
    21001 cattacaact atatcctaca gatttacaac caaattcagt ttttatttct tcagctactt
    21061 tttttgcgtt gtcttcattt aaatctgcaa tagcaacatc ataacctact ttagctaaat
    21121 gtttagataa atattcacct aaacttcttc ctcctccaac gactaaagcc acttttctca
    21181 ttttcatttc ccctttttta tcacattaaa agttaacttg attagttaaa ttgactttta
    21241 tctataattt acttataata tgattagatt cgttttaaat tttattttct aattattttt
    21301 agtatagtac ctacgccaat aggtgctatt tctttttctt caagatataa ggttcctcca
    21361 attgctccat ctttttcacc tgtaaactta aatgttatat gacctaattc tgaaagattt
    21421 tggtttacta actcaccaac agaagtaatt ttgtaatcaa cattatttat tgatagcaca
    21481 tcatccgtaa gtatttcttt tactaaatta ttttcagaat gtatataaca gtaattttct
    21541 aactcttgag gagcattatc tttgaaagtt acaaacatat tatgttgtaa aaattcattt
    21601 gcattatctc ctattttatt tatttgagta ctgtatatta attccatatc aactgaacct
    21661 ctctctaatt atttataaag tattcttcta tttatataac ccaaagcttg ctaaccatgc
    21721 taatagaaca gttggtgcac ctgttaaaaa tctagaatat aaaactgacg gaactcctac
    21781 ttgaactgta tcaggttcag cttctgcaag accaagacct actggtataa agtcacaagc
    21841 tgcttgtgag tttattgcaa acaatgctgg taatgctaaa cttggtggaa tatttccttt
    21901 tcctatttct gtaccaacca atacccccac tatctgagct ataacagctc ctggtccaag
    21961 aaacggagac aacaacggaa atgaacatat tattgataag acaactaaac ctccaacatt
    22021 tccgattaat ggagtaagta aattggctac ccaatttcct atacccgatt gatttattat
    22081 acctattagg actgcaacga aagccataaa aggcagtata gtatgtatta atgtatctat
    22141 agtatcccta ccagcttgaa aaagagtact tgtcacattt cctagtgcta tacctatttt
    22201 tgcaacaatt ccttgactct gctcacttat cttcttttta gtatcataag ccatatttct
    22261 tccctcctac taccttaatt ataactgctc tacatttgtt ggttttaccc ctgaaacata
    22321 tatatcttct tttataaaca ttgctaatgg tccacttttt cctgttttca ttatatttat
    22381 agtcggtatt ttcttttgag gatatatacc acatctcaat gttccaccac aatttattat
    22441 tacacatgcg atttcatcat ctttaacacc ctgtgaaaat ccatctatta actcacatcc
    22501 tgtaagttca gctattttta ctgcagtttc tggctttgct cccccagtca catatactac
    22561 cttattcttc ttttctgttg gttctattat caatggtcca ccccatcctc cagaaccttt
    22621 tactatttta actttgttaa atgccatata atttatcctc ctctatacac tatcttttta
    22681 tttgaaaaat ttatttatac cttaaagtaa ttatccaatc tttatatctg aatttaatgc
    22741 tattccttgt tgtttctcta caaattttgt tgtatagtca gtaactacac ctttaataaa
    22801 gtttgcaact atacccacta acaaatatct aactgctaaa tctgctgttg agaatcccaa
    22861 agttgttata ccatttgcaa ttcctagata tataaatagt tcacctggat tgatatgtgg
    22921 gaataatcca ttcattgtat ggcatgaaaa gcttgctgct gcaaagtaac taggtttaaa
    22981 cttttctggc aagaatcttc ctaatgatag tgtcattggg tttgcaaata caaatgtacc
    23041 aaggactggt aatatcaaat atctagtaaa tatatttgca gaacattttt ttgcaagtct
    23101 ctcgactcta tctgtaccaa taaagttgat aattgcattc attgtaacta ataatgctac
    23161 taaaagtggt actatatctg ttacaaaacc tacaaattgt tgcccacctg ccctaaataa
    23221 attcataaaa ctatctgcac cacttgctaa tacttgaaaa aattgcgtca tattaactcc
    23281 tccttataaa tatatttttt taaactactt ccatcctaca ctcttcatca gtactcagat
    23341 tatttgtatc ttcacattta tttttctcaa atgttatgta ctgtttgact gcatctagta
    23401 ttgctttttt aagtagttta ttgtaaacag ttaaatctga tttttctata tactctaagc
    23461 tctttcctac caaaccttca aatgacttta ctcttgccaa tactgtaaca ccttgcattt
    23521 ttctagtctc gacaatcaca ccatcatcat ttataagtat caacacaatt gtacccgatg
    23581 aaattcttcc tttttttctg ccaattgcca ctcttccttt tctttttagt tcaatataat
    23641 ttttattaaa atctttaagc tgtaagtatc caagaaaaat atttaataaa tacactgtaa
    23701 taagtgatgc tataataaaa attactgggt ccatattatt acctcctctt ggtaattatt
    23761 ttataaaatt catcaaatga ttttacctta gcaagttctt ctaccatctt tttatctctc
    23821 cctacttcaa gtacttcttc ataacttttt attatcaggt ctgtactatc tacattctta
    23881 tctggaatta tcatcaatat tattattcta atttcctttg tatcccaaat cactggtttt
    23941 tctaatattc caaatgccag ataaagattt tccccattag tattaactga gtgaggcaat
    24001 gctatataat tatctaatgc cgttgaagat ttcttttctc tttttaatac cttttttttg
    24061 aactctatat caataattcc tttatgaact aatttttcta acatatcttc taaattttcc
    24121 ataaacgtac tcttattcaa tataaagaac ttatcctctt caattaattt acctataaaa
    24181 gggatatttt tttcaatctt ttttatagac ttaagattta tactattttt caaaagtatt
    24241 gcattttcta tctctttctt tatactttct tcattaaata acgcatttac tctaaaaatt
    24301 ggaacatcta agtctgtttt gaaatctact gttgtaaata ctaaatcata atactttaat
    24361 aaatctaaat tagctttagc ttctatatca gaaaaagtat ttacttctac actttctcca
    24421 agtatttttc ctatctttat tctaataagt tgtgcagtcc caagacctgt tccacaaaca
    24481 acagctaccc tatttacatt tagaactttt cttgaatttt tttcaaaata acttccaaaa
    24541 tgtaatgcta tatatcctat ttcgtggtca catattttca agtcaaactt ttcctcaatt
    24601 atcattgcag ctatctttgc aacactatat ggaaatgtat atctttcctt tatttcttgt
    24661 aaaagaggat tttttaccct tatgttgaat ctcattctat taagtgcaaa atacaagtga
    24721 tattccaaat tttctataag ctttttatct tctttaaaat caatccctga ggtctctaat
    24781 aaaaaactag ttacttcatc tacaacttcc tttacgctat catttatctt tatactgcta
    24841 agtgctaatt ttgtactagg agcttttcta cctattaatg gcattgtcaa aaatatggtt
    24901 tctttttcat ttaaaactat attaaatatc ttttccaata aagctttgag ctttaatcct
    24961 aaagtatatt catctgaaca tcttatcact tcaaatctct tatctatctt ttcaaatttg
    25021 tacccattta gatttcttaa gacagacatg attatatatc tttcaacatc tctcaagacc
    25081 tgttctgtta tgtagagttc ttctttttta aataactttt ttaattcatc tctaagacat
    25141 tcaatatcat aatccttaac taaatttatc tgattaacgt tttccaagac atcaatataa
    25201 ttttcataca atctatcaag gattaataat cttatatcca tttcttcacc aactaaaatc
    25261 atacctatat ttggcttcct ttttaattct aaatcatatt cagctaaaat tctgtctgct
    25321 ttttttaaat cctttattaa tgttgttctt cctatacata tctcttcagc caaatcatct
    25381 atggtaattg gattgttggt catagttaaa atatcaagaa cataatttat tctttcatca
    25441 ggtgaattta aatttataaa atttgatagc tttcctctat taaaatttac aatctcaatt
    25501 aatttacttt catcctcaat tcttatacta tatgtacctt tttcattttt aactatttta
    25561 gcaataccac cttttaattc tcgattaatc tggtttatat agtttctgat agtcctagta
    25621 ctaacaccca aaacatttga aaattgttct agagtatttg tgtcttcttc taacaccatt
    25681 tctaaaatca aaaaaaccct agggtccata taagtcattg acatcttttt ctcccctttg
    25741 ttatcttaca ttttttatta taatctaaac tatatagata attaatattc tctttttcaa
    25801 atcagatttg aaaaaaagga actaatatac atttttcaca atactctgga aaattataac
    25861 atcatataaa acaagaaaag ccttatgctg ttaactctta cagattatct actatctgtt
    25921 ttgaaaacac atcaggcttt ttaaatatcc aatatattat tttatatatt ttctaattat
    25981 agttttgtat actatacgtt attttttctt cttatttgct ttttctatat taatcttatt
    26041 accttttatc ttaatatctt tcatcttttc aattactgtt tttgcatctt ttttaggtat
    26101 ttcaacaaaa gtatacttat catatatatc tatagtacct actactttac ctggtattcc
    26161 aacttctcca gctatggcac caactatatc ttttgcttgt actctttgat ttctaccaat
    26221 attgataaat aatctaatca taccatcttt agctccagta ccaccttttc tatctccacg
    26281 ctctcttcta ggtttttctt ctattatttc ttctttcatt tcttcaccta gagatagttt
    26341 tacaagtgca gcagctatat caaccattgc ataatcttca tcattacaga aaccttctaa
    26401 ccattgtaat tgtttagtta agtgaccttc ttctatagtt tgtttaactt gtgcaaagaa
    26461 agtacctact tttttctctt caacatcagt tattgttggt atattatgct ttattagttt
    26521 agatttagta tatctttcta tatctttcat ctttctcatt tcttttccaa atacaaaagt
    26581 aaatgacata ccttctctac cagcacgacc agttctacca attctatgaa cgtaatactc
    26641 ttcatcttgt ggtaagtcat agttgaatac acattcaaca tcatcaacat ctatacctct
    26701 agctgcaaca tcagtagcaa caagtatatc tattgtacca tttctaaact tatccattac
    26761 tatatctctt tgagtttgtt ttaagtcacc atgtaatgca tctgcaaaat atcctcttgc
    26821 ttgtaaatct cctaccaatt catctgcttt tctcttagta ttacagaaaa caacagatag
    26881 tttagggtca tatacatcta ctaatctaca taatacttct aatttatttg atgacctagt
    26941 ttctatatag tattgctttg tatttgaaac tgtaagttct tttctaacaa cttttatatg
    27001 ctctgggtct ttttgatatc tcttagttaa ttccaaaatt cctcttggca tagtagctga
    27061 aaagaaagtt gtttgtcttt cttctggaat cttacttaaa atcatttcta tatcttctct
    27121 aaatcccata tctagcattt catctgcttc atctaatata atcattttta cattatccat
    27181 ctttaatgtt ttacgattga tatggtctat tgtacgtcct ggtgtaccta ttacaacttg
    27241 cacaccactc tttagagatt ttatttgtct atctattggc tgaccaccat atataggtaa
    27301 tgtctttatt ccgtgcgaat acttagctaa ctttcttatt tctgttgata cttgtattgc
    27361 cagttctctt gttggacaaa gcactacagc ttgtaaactt ctattatttg ggtctattgt
    27421 ttctagtata gggatactaa aagcagctgt ttttcccgta ccagtttgag cttgtcctat
    27481 aacgtcttta cctgacaaaa tcgcaggtat agactgtgct tgtataggag atggttcttc
    27541 aaaccccatt tcagctatag ctttttttat tccctcactt attggtaaat cttcaaactt
    27601 tgttatattc atatattatt cctctccgtt atcaattaat cttaacctat ccattatata
    27661 cttttttagg atattctgca attaatattt taaacagtat agttaaataa tatacatgaa
    27721 atacaaaatt gatatgtaca gaaaatatgt gaataacatc aatatacctt gtattctact
    27781 aagtttatta gatttaagtg ttggtattat tactattaat aataataata tcatgaatgg
    27841 tatatccaac tgtctatttt gagatagtat tggtatgtta tttggtattg ctgatacagc
    27901 tattactgat actatattca atatgtttgc acccaaaata tttcctactg atatttcatg
    27961 atgatttttt cttatagcag taatcgacga aactatctct ggcagagaag ttccaagtgc
    28021 tattatagtt aaacttacta taccttgagg tatatttaaa aaactagcta ttattactcc
    28081 actctctata aggatttgtg aacctattat catcattatc aatcctgtag caaaaagtaa
    28141 tagtattttt acaatttcta atacggaaaa acctctataa tttttccctt ttttagtaga
    28201 tatgtcttta atagtgttat ttttaatatt tctattttta gtatcagatt ttccaacgac
    28261 acttctataa ttattaacca tataaaagat taatattatt ataagtaata atgaatcacc
    28321 tcttgttata actccatcta aactcaataa aattaaaact ataacggata ctattaatag
    28381 taaagactta gaaaaaaaca tttttttatc tacattaaat ggacttataa aaacaaccaa
    28441 ccctaatgct aaccctgtat tacatattat agaaccaact gcatttccta aactcatagt
    28501 agtatgacca tcaatagaag caagtagaga cactgtaaac tcaggtaatg ttgttgcaaa
    28561 actaactata gtagctccaa gtataatttc tgatatattc gtttttttac ctatctctac
    28621 agtacaattt ataaagatat ctgcgccttt agtaattaat aggaaaccta ctaaaaataa
    28681 taccactgtg ataaacaatc tatcacctcc ccctattaaa taaatattca gtttgtgaag
    28741 atgtatgctt tttctttttt ccataataaa atttatttat cctaaacaaa aaataaaaaa
    28801 tattttctcc ttaattagct atttatttgt tttattttat ttatatatgg gtataatttc
    28861 accacattat taacatataa tctgttttat actttaaagc gacaattttt tattttttat
    28921 gtatacagga tacgaataat atgctattat tatattagaa aatatatttt taaaaaaata
    28981 tataaaataa attaagtata taaaaattaa ttttaatagt attatttatc atttaaatct
    29041 aaggaggaaa tacttaatga atgcatggca aggatttaaa acaggaagat ggactaaaga
    29101 aataaatgtt agagagttta tacaattaaa ctattctcca tatgagggaa atgactcttt
    29161 cttagctggt gccactgaaa atactaaaaa attatgggac gaagcaatgg tcctatttaa
    29221 aaaagaaaga gaaaatggtg gtactttaga tgttgataca aaaactgttt ctggaatagc
    29281 tgcatatgct ccaggatatt tagataaaga attagaaaca atagttgggt tacaaacgga
    29341 tgccccttta aaaagagctg tcatgccata tggtggtata aagatggttg aaaattcatg
    29401 cgaagctttt ggatatgaat tagacccaga aataaaagat atatttacta aatacagaaa
    29461 aacacacaac caaggagttt ttgatgttta tactccagaa atgagagctg ctagaaaatc
    29521 tggtataata acaggtcttc cagatgctta tggtagaggt agaataattg gtgactacag
    29581 aagagttgct ctatatggtg ttgatgcttt aatagaagat aaaaatgagc aaaagaaatc
    29641 attagaagtt tcttgcatgg atgaggaagt tatcagatta agagaagaaa taactgagca
    29701 aatatcagct ttaaatgaac ttaaaaaaat ggctgaatct tatggatttg atatctcaaa
    29761 accagcaact aactcaaaag aagctgtaca atggttatac tttggttatt taggtgctgt
    29821 taaagaccaa aatggtgctg caatgtcttt aggtagaaca tctactttct tagatatata
    29881 ctttgaaaga gatttaaaag ctggaatcgt aacagaagaa gaattacaag agtatatgga
    29941 ccattttgtt atgaaattaa gaatggttaa gttcctaaga actcctgatt acaataacct
    30001 attctctgga gacccaactt gggtaactga gtgtatagga ggtatgggaa tagatggtag
    30061 aacattagtt actaaaaact catttagaat gttaaatact ctatatacat taggaccttc
    30121 tccagaacca aacttaacag ttctatggtc aactaaatta cctcaaggat tcaaagattt
    30181 ctgttctaaa gtatctatag atacaagttc agtacagtat gaaaatgatg atttaatgag
    30241 agcatactgg ggagatgact atggtatagc ttgttgtgta tctgcaatga gaattggtaa
    30301 acaaatgcag ttctttggag ctagagttaa cttagctaaa actttattat acgctataaa
    30361 tggtggtgta gatgagaaat ctggtgttca agttggacca agatttgaac ctataacttc
    30421 tgagtactta gattatgatg aagttatgag taaatttgaa ccattcactg attggttagc
    30481 aacattatat gtaaatactt taaatgtaat acattatatg catgataaat attcttatga
    30541 agcattagaa atggcattac atgatagaga tatatttaga actatggctt gtggtatggc
    30601 tggattatca gtttgtgcag actcattatc tgctataaaa catgctaagg ttaaaactat
    30661 tagaaatgaa caaggaatag ctgttgattt tgaaatagaa ggagattatc caaaatacgg
    30721 aaataatgat gatagagttg atagtatagc tgtagagtta gttgaaagct ttatgaataa
    30781 aataagaaag aataaaactt atagaaattc ttatcctact caatctatac ttacaataac
    30841 ttcaaatgtt gtttatggta agaaaactgg taatacacca gatggtagaa gagctggtgc
    30901 tccatttgct cctggtgcta acccaatgca tggtagagat actaatggtg ctttagcttc
    30961 attatcttca gttgctaaat taccatatga acatgctcaa gatggtatat ctaatacttt
    31021 ctctatagta cctgctgctt taggaaaaga tatgactgaa agaataaaca atctttctgc
    31081 aatgatggat ggatacttcg ctcaaaatgc tcatcactta aatgtaaatg tatttgatag
    31141 agctacttta gaagatgcta tggaacatcc agaagaatat ccacaattaa ctataagagt
    31201 atctggatat gcagttaact tcataaaatt aactaaagag caacaattag atgttataaa
    31261 cagaacattc cacggtaaaa tggcttagtc tttaaatttt tatactttaa ctaagattac
    31321 tactgtgtaa aaaataaaaa taaaaggcta tcttatattt gaattatttc tggtataaga
    31381 tggccttttt tagagaaata ttataactat tgataaggtt attaaaatat attgtgaggt
    31441 gaactctatg attaagggaa agatacattc tatagaaacg tttggtactg tagatggacc
    31501 aggaataaga tatatattat tttttcaggg gtgtcctctt agatgcaaat actgtcataa
    31561 cagggataca tgggatatta aatcaggaaa agaatacaca gttgatgaaa taataacgga
    31621 tgctcttaag tatacttctt ttatgaagtt ttctggtgga ggtataactg cctctggtgg
    31681 tgaatccact cttcaaccag aatttttaag tgaattattt aaaaaagcaa aagaaaatga
    31741 tatacacact tgcttggata catctggatt tgtagatata gaaactatag acccagtttt
    31801 agataatact gatttggttt tacttgattt aaaacatatg gttgaggaaa aatctattga
    31861 tttgactggt gttggtatgg ataaggcatt aaaacttgct agacacttag aatcaagaaa
    31921 tattcctgta tggataagac atgttttagt tcctggcatt actgatgata tagataattt
    31981 agaaaaacta ggtcaatttg tagctacatt gaaaaatgta gaaagatttg aattgctacc
    32041 ataccattct atgggaatac acaaatggga aagtttaggt atcgattatg aactaaaaga
    32101 tgttcctgat gccactaagg aagatgtcca aaaagctagt gaaataatct ctaaatttgg
    32161 agtgaaagta tataatagtt agtgtagtat attaatcctt tatagaagac ataacaaaat
    32221 agggtaaagt gtaaattttt aataaacttc aaaacttcca ctttgcccta tttttatata
    32281 ctatatatct cattttatat acatcacttt gatagctgaa atttataaaa ttcagaggca
    32341 cttacaggtc ttccaaataa aaaaccctga ataatatcta cccctaagct ttttactata
    32401 tcatactctt cataagactc tattccctca acacaaactt ttaaatttat aatatgagct
    32461 aatttaataa tatattcaag aaaagtatat tcataactat tatatgtaat attcttaaca
    32521 aaacttcttt caatttttat tatattaaca gacagttctt taaggtaatt taaagatgaa
    32581 tatcctgtac caaagtcatc tattgcaata tatactccta taccctttaa actaataaat
    32641 ttatcattta gtaaatttat atctggtatc caacaattct ctgttaattc caaaattaaa
    32701 aattcaggtc ttaattgata ctctactaaa cattctacta taaaatctct aaaaaaatct
    32761 tcctttaact gaatatacga tacatttaca gatattttaa agtctggatt tattttatgc
    32821 cattctttac actgttttac agcttctttt ataatccact tcccaactgg tataattaaa
    32881 ttactctgtt ctagtattgg tataaattca acaggagata cttctccata agtactcgaa
    32941 tgccatctta aaagtacctc tgcccctatt acctcttttg taacagcatt tacctggggt
    33001 tgaaaaaaca actcaaaatc attaaaatta ttttcaacgc actctcttag tttttgttgc
    33061 attgaaatta cctttagttt attttcatag agctcttgtg aaaaaaactt aattctattt
    33121 ttaccactaa tttttgctat atccaatgca atatcagcat gtttaaataa atctaaataa
    33181 ttatctccat cttctggata catagcaact cctgcagtca ctgtgcaata gtacttattt
    33241 gattcaattt catgctgagt atttgtatac atctgtatct tttcatatat tttctctata
    33301 gtttctttag tacacatagg gtaaaaaaat gcaaattcat ctccatctaa cctatacaat
    33361 cttacatctt ttggaagata ttccagtact tcagtagcaa ttgcacgcaa cactttgtca
    33421 ccataagaat gtccatattt ttcatttata tttttaaaat tatcaatatc cataataaac
    33481 atagcaccat ttttataaat tttctctttt ataagataat tcattctatg ttcaaattgt
    33541 tctctattcc acgttcctgt gatagaatca aatttatttt tttcaccaat attctttatt
    33601 ctcccgacta gaaatatagt ttttgggtca tccgacacat aaactttacc tctacaagaa
    33661 atccatacta tttcttcata tttattaatc agcctatatt ctaaattgtg ctcacctttt
    33721 tttccatgta gtaattcttg tatatcatct ttccatattt gaacatcatc cggataaaca
    33781 attttactcc aacaattgac caaatcagat tctatctctt tacttaagtt aaattcatca
    33841 aaaattgctg aagaaatttt aaatttattc ctatttatat cccaaaaaaa tatataatct
    33901 tctgtacttt gagataaaag ttcaaagaaa atttctatat ggtctaaact taaagagttc
    33961 gattcttctc tcatcttttc cccctacaat attactatta gtgtagttta atcaacaaaa
    34021 tatccttatt tagattatac tattttacta attttttgtc tcatcaaaca catttcccca
    34081 actttagtta tcttatcaaa tctcttactc ttaactaagt tttttgagat tcataaagta
    34141 atttttctat aatcattact ggtgatttat ttttacctgc taaaacacca tgttaaatca
    34201 tttattaaat cattttctct taaaattcga tttttcgtta tataaagagt tatcagttct
    34261 ttattctata aaacttttct attactcttg aatttatttt atttttttct ccgcctattc
    34321 tctttggatt tggtattgtt tttctataga ttttggcaaa gaagtgtctc ttcctataat
    34381 ataagatttt gatatttctt catcatctat aaatttttta atagggatta tatcattttt
    34441 atatttattt atataaatat tttttgtttt taattttttc tctcttctta tgtacttaca
    34501 tttaatttat tcttttcagt taataaaata ggtacatttt tagtacatga aaatagagtt
    34561 gc
//
LOCUS       DAELJO010000020        34228 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELJO010000020 DAELJO010000000
VERSION     DAELJO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.020"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            115..498
                     /locus_tag="KPB88_000868"
     CDS             115..498
                     /locus_tag="KPB88_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053169.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            1006..1389
                     /locus_tag="KPB88_000869"
     CDS             1006..1389
                     /locus_tag="KPB88_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0053170.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1391..1741
                     /locus_tag="KPB88_000870"
     CDS             1391..1741
                     /locus_tag="KPB88_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053171.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(1842..2300)
                     /locus_tag="KPB88_000871"
     CDS             complement(1842..2300)
                     /locus_tag="KPB88_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053172.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2345..3133)
                     /locus_tag="KPB88_000872"
     CDS             complement(2345..3133)
                     /locus_tag="KPB88_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0053173.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3272..3709)
                     /locus_tag="KPB88_000873"
     CDS             complement(3272..3709)
                     /locus_tag="KPB88_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG0053174.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4531..5040
                     /locus_tag="KPB88_000874"
     CDS             4531..5040
                     /locus_tag="KPB88_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053175.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5438..5974
                     /locus_tag="KPB88_000875"
     CDS             5438..5974
                     /locus_tag="KPB88_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0053176.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKI"
     gene            complement(6408..7208)
                     /locus_tag="KPB88_000876"
     CDS             complement(6408..7208)
                     /locus_tag="KPB88_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053177.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7403..9001)
                     /locus_tag="KPB88_000877"
     CDS             complement(7403..9001)
                     /locus_tag="KPB88_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0053178.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            9329..9505
                     /locus_tag="KPB88_000878"
     CDS             9329..9505
                     /locus_tag="KPB88_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053179.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            9679..10809
                     /locus_tag="KPB88_000879"
     CDS             9679..10809
                     /locus_tag="KPB88_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0053180.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            11050..11400
                     /locus_tag="KPB88_000880"
     CDS             11050..11400
                     /locus_tag="KPB88_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0053181.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            11408..11857
                     /locus_tag="KPB88_000881"
     CDS             11408..11857
                     /locus_tag="KPB88_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG0053182.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11959..12534
                     /locus_tag="KPB88_000882"
     CDS             11959..12534
                     /locus_tag="KPB88_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0053183.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12527..13936
                     /locus_tag="KPB88_000883"
     CDS             12527..13936
                     /locus_tag="KPB88_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG0053184.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            14120..15553
                     /gene="eutA"
                     /locus_tag="KPB88_000884"
     CDS             14120..15553
                     /gene="eutA"
                     /locus_tag="KPB88_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG0053185.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVVVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15571..16935
                     /locus_tag="KPB88_000885"
     CDS             15571..16935
                     /locus_tag="KPB88_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG0053186.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16948..17829
                     /gene="eutC"
                     /locus_tag="KPB88_000886"
     CDS             16948..17829
                     /gene="eutC"
                     /locus_tag="KPB88_000886"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG0053187.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17849..18502
                     /gene="eutL"
                     /locus_tag="KPB88_000887"
     CDS             17849..18502
                     /gene="eutL"
                     /locus_tag="KPB88_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG0053188.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18513..19214
                     /locus_tag="KPB88_000888"
     CDS             18513..19214
                     /locus_tag="KPB88_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0053189.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKEIVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            19217..20686
                     /locus_tag="KPB88_000889"
     CDS             19217..20686
                     /locus_tag="KPB88_000889"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG0053190.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20782..21069
                     /gene="eutM"
                     /locus_tag="KPB88_000890"
     CDS             20782..21069
                     /gene="eutM"
                     /locus_tag="KPB88_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG0053191.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            21196..21957
                     /locus_tag="KPB88_000891"
     CDS             21196..21957
                     /locus_tag="KPB88_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG0053192.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21970..22599
                     /locus_tag="KPB88_000892"
     CDS             21970..22599
                     /locus_tag="KPB88_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0053193.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREYVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22641..23366
                     /locus_tag="KPB88_000893"
     CDS             22641..23366
                     /locus_tag="KPB88_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG0053194.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            23379..23651
                     /locus_tag="KPB88_000894"
     CDS             23379..23651
                     /locus_tag="KPB88_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG0053195.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23644..24195
                     /locus_tag="KPB88_000895"
     CDS             23644..24195
                     /locus_tag="KPB88_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0053196.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            24207..25298
                     /gene="eutH"
                     /locus_tag="KPB88_000896"
     CDS             24207..25298
                     /gene="eutH"
                     /locus_tag="KPB88_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0053197.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            25300..25773
                     /locus_tag="KPB88_000897"
     CDS             25300..25773
                     /locus_tag="KPB88_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG0053198.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25893..25994)
                     /locus_tag="KPB88_000898"
                     /pseudo
     CDS             complement(25893..25994)
                     /locus_tag="KPB88_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            26289..26810
                     /locus_tag="KPB88_000899"
     CDS             26289..26810
                     /locus_tag="KPB88_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0053199.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            27189..28364
                     /locus_tag="KPB88_000900"
     CDS             27189..28364
                     /locus_tag="KPB88_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053200.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            28398..29936
                     /locus_tag="KPB88_000901"
     CDS             28398..29936
                     /locus_tag="KPB88_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0053201.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAIAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29968..31170
                     /locus_tag="KPB88_000902"
     CDS             29968..31170
                     /locus_tag="KPB88_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0053202.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(31337..31936)
                     /locus_tag="KPB88_000903"
     CDS             complement(31337..31936)
                     /locus_tag="KPB88_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0053203.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            32106..33464
                     /locus_tag="KPB88_000904"
     CDS             32106..33464
                     /locus_tag="KPB88_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053204.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            33457..34089
                     /locus_tag="KPB88_000905"
     CDS             33457..34089
                     /locus_tag="KPB88_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0053205.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT        13419 a         4183 c         5711 g        10915 t
ORIGIN      
        1 tatattatac tatcttttct ttttggaata aactatactt taaatataga tatttaattt
       61 aaataaattt tacagtatta atagtaaatt atcttagaaa ggaatgtgct ttatatgtct
      121 acagacaaag ttgttggaat catagatgaa gaaacggcag aacttgcagg aattgaatat
      181 actggtaaaa tttatgcttc ttctggtgtt ataaaacata taaagaaaaa acatagatgt
      241 caactgagta aggatatttt taatgatata atagacacta taaaaatggt attaaagtca
      301 cctgaatata taggaagtca tcctaaaaaa cctggaaaaa gtgtggagtt tataaaaaaa
      361 attgatgatt atattttagt agctacagaa ttagatacaa aaaaaggata tttatatgta
      421 tcttcaatgt atgctataaa agaagcaaaa ttagaaagta gagtaaacag tggaagagta
      481 aaacaatatg atggttaaca taatattatt aattttccct aaaatattga aaaatattat
      541 tatataagtt ataatattaa tatacacgta gatatttatt agttagattt gaggttggaa
      601 aaggcaccca atacgctact atagagtagt taatagagat gcaggatacg ccgccctgcc
      661 aaatctaatt aaattaaaaa gagaggatta attcctctct tattgttttt ttgagatact
      721 aatttaatct ataatagaaa gattgaaggt cgtttataca aaaaaatttt cagttatata
      781 cattaaacta attaaataag atatttataa tctatatgtg atacatatta tatattaata
      841 aaagaaaaat atatagaaaa tccttataaa aatttaaatt gtaatgaata ggaagtaatt
      901 aaaaagaatt tttattgatt tttaaaaatt tagttagaaa ctatatcatt atggtaaaaa
      961 atgtattata atgtttagaa atattataat aaggagcgat taaacatgaa tctatcgaag
     1021 aaaatagaag ataaaagaaa agctaaacag agagctgaaa aagttaagaa agctaaaatt
     1081 gctacagctg gtgtggtatt aggagctgtt actggtgcag tgagtggagt acttttggct
     1141 cctaaatctg gaaaagaaac aagagaggat ataaaagatg cttcacaaca aattgctgaa
     1201 aagataaata tgaaaactgt agatgtaaaa ggcaaagtat ctgagaagct agaggataaa
     1261 aaaggaaact ttatagaatc aaagaaaaaa ataaaaaaat atatagataa caaaaagagt
     1321 gttgaaaatt ctagtgagga agataaaata atagatatag ttgaaccagt tagtgttact
     1381 gaggaataat atggacaata caataacgat taacataagt ataatatatg tattattagc
     1441 tatacttgct tgtattgctt tagtttattt aattattgta cttgttaaac tacataagat
     1501 tttaaataat gttggaacta tgttaaatga aaatactcaa aatataaata caactttaaa
     1561 taatcttcct aatgttgtag aaacttttgg agaagtagga gaaaatatga aagatgtaac
     1621 agaagtagtt actgaagtta cagcagattt tcttgttact aaagacagtg tcaaatcaaa
     1681 tatggatgtt atagttgata tattgactat aattaaaaac atttttgtag ataaaaaata
     1741 ataagttaat ataaaataag cctataagat ataatttaaa cgaccttaaa atagcattta
     1801 taaggtcgtt ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc
     1861 ctctttatca tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca
     1921 tctttacatt caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa
     1981 aagatataag aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa
     2041 ctccaaaata atccacaacc tgatatataa gatgctctac tttgatttat ataaacctca
     2101 tttagaagct tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt
     2161 aattcattta aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta
     2221 taattttcat caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct
     2281 acaacttttg ttcttatcat ttttttatcc taacttattt attttttata aaataatcat
     2341 gattttataa tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac
     2401 catttattac tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct
     2461 ttttacctaa accttgctta taactagacg cctcaattat taaagaatct ttcaattgcc
     2521 aatgtaaaat ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat
     2581 acactttatt ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg
     2641 ctatcaattt agcaacttta tctttattat taacataata atctgtatct gctttactat
     2701 ctacaaaaca tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac
     2761 ctctaacatc agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc
     2821 tttctgcata ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg
     2881 aagttgtagc atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg
     2941 ctatagcaca ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag
     3001 tagctccacc tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc
     3061 tttcgtttat atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta
     3121 ttactacttt caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc
     3181 ataacctaga attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa
     3241 aatatagaat attaagtaaa taatgttaaa attataattt tgtatataag acattacttg
     3301 attgcaataa ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt
     3361 taattccagc ttgaattata gccttagtac aatttaagca aggaaagtga gttacataaa
     3421 tagagcaatt tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag
     3481 cgtgaatggt tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct
     3541 tatctccaga aatactacca ttatatccag tagacactat tctgttttct gaattgacaa
     3601 ttatagctcc tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc
     3661 tcataaaata ttcttgccat gagcatctat tttcaagttt tttattcaaa ttatatacct
     3721 ccaaaactta tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt
     3781 atacgataaa taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa
     3841 agttacaagg aatctaaaat tatatacatc tttcttcgta atatgaatgt tacaatataa
     3901 aaaactgtct caataaaata gagacagttt tacagtcaaa gtttataata caagttcata
     3961 tttttcttta aattctttta cagcttcggg ccattccttt tgttctgtaa tacctttttc
     4021 tttagctatt ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt
     4081 taatctccct tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg
     4141 ctgcatatac aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct
     4201 ttaaccgaca tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt
     4261 tttatttttt tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata
     4321 aatatttcta cagttttaat tactggacat atctattttt attataaaaa gcgcttaaaa
     4381 atcgagaata tccgcataaa aactgtatat aagcaaaacg gttaaaggaa aaagaagaaa
     4441 tttacttgaa tgttaaaatg cgttgcttga agcaatgggc agttttgcct ataatatcgg
     4501 taacaactga aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg
     4561 aagtcaaaag gactttcaca acaagaactt gctatcaaac tgaatgtagt gcgacaaaca
     4621 atatctaaat gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca
     4681 gaggtgcttg aaacgcccgt aagcacttta cttggagaaa ctattgttga gtcggaagtt
     4741 gatagcttaa aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga
     4801 aaaaacatga gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatatta
     4861 atagtttttg tggtcttagg gggactaaat agtccttact tggattggaa ttataccgac
     4921 cctgaaactg ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta
     4981 gtaccgattg tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga
     5041 agtaactctg ctttttataa tacagcaagc gttccttaaa agtgtcattc tacatggcaa
     5101 tcgaggacaa ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg
     5161 acgacagtaa gaccaaaacc gcattaagta aagtatagga aacaagactg ctggacttat
     5221 actctcatgc gtcagatgca ggaaatgcga ggtctggtag catgatttac aacgatttgt
     5281 acaacgacta cgagtagaaa tatatggaga taagtattat ctacgaaatc agaaaagaaa
     5341 tgacttgata atttactcat gttataatta ttgatgtgaa atgaataaat caaaattcct
     5401 ttttgggatt taacaataag gaaaaagtga atgacttttg caaggaggtg ttaatatcag
     5461 acaagtaaag tcttttgttt ttccagttat atccttaata ttcttagacc aaattagcaa
     5521 agttcttata ggattattct taatggactt tgaaattgat ataattggga aatttttaag
     5581 attcaatcct gttcaaaata caaatctctc ttatggggga aactttattg gtattctatc
     5641 taatttatgg gtattggtat tgtttaacat tttagttata ttagttatta tatctggata
     5701 tgctttttat aaatcaaaaa atgaacaaac aagctattca gtaaaagtaa ttatgtcttg
     5761 tggacttgct ggtacaatat gtagcttgat agataaatta ttttggggag gaagtttaga
     5821 ttttttgcag ataccaagct tttttatttt cgacttaaaa gactgctacc ttactgttgc
     5881 agaaataata tttgttgtca taggaatttt gcataataga gaaatatcaa tgaaagaata
     5941 catatatttt tgctatcgtc agtttaaaat ataaatttag aagataaatt taagtttcaa
     6001 ggtaaactta tttaatcaca gcaaatagtt ggcttactta agcttttggg ctaaactgtg
     6061 agcactaatt aagtctctgc tatctaacaa acggagcaca aaaataaaaa ttaaatatta
     6121 aatataaagc acagttaatt ttgattaaaa agttgactgt cttttctttt gtagaaattt
     6181 aagcataggt aataaagaaa tgactgcatt taagcattat gtatataatt ggaggcaata
     6241 aaaagccagc aaagcccata aaatagagct gtgctagcaa catgatgagt tataagaaga
     6301 taatcaaaaa cttacgcttt attttaattt aaaaaaatga ttactgtaaa gaaatcattt
     6361 tttattataa tcactgatac atcaaatatt ctaaataaaa tttttattta tctggaattg
     6421 tatatttttt ctataatatt aactacagcg tacattgccc aagcacagat agccaataca
     6481 aatactccca tcattactaa atctaattta aatacttgag aaccatatac tatcaagtag
     6541 cctattccat atcttgaaac taaaaattct ccaactatta cacctaccca tgccatacca
     6601 atattaattt tagttaaatt tattaaattt cctgtgtttg caggtaatat gagtttaaat
     6661 aatatttgag atttagtagc tccaaagctt tttaacatct taattttttc ttcatctata
     6721 ttcataaaat aattataagc tgaaagtata gtaacaacaa cagaaatcgt tacagctgtt
     6781 acgacgattc cttctattcc agcacctacc caaactataa ttataggagc taaagcagtc
     6841 ttaggaagag catttaaaac taccaaaaat gggtctaata tttttgataa tttttctgac
     6901 caccaaagag caattgctac caaaatacct aatacagtac ctataacaag accaagaaca
     6961 gtttcatata cagatattcc aatatgttta aataattgac cactaaatgc atattgaata
     7021 aataaattgt aaatatcact tggtttgcta aatagaaatg tatttataac atttaagtca
     7081 gctaaaagtt cccagattac aataaaacca attaaaatta atagctggta gaataataca
     7141 tttctttttt ctctttttac acctttaaca tagtttatat atccttcaga tcttttatta
     7201 agcctcatgt gtatctagct ccttccataa tatgtcgaaa tacttttgaa aatcaggaac
     7261 tttacgtgtt ttgagaggcg tttttgttcc atccatgtta agttcaatat cgtaaatatt
     7321 ttttatggta gctggtcttt tagatagtat tgcaacctta tctgatatgc ttattgcttc
     7381 tgctatagct aaaaaagtgc aactaattgc ttaaagaaac agaattatca acttcagcac
     7441 ccatataaac aatattaaga tttcctaaac tgctatccca aacgatacta ttaataatag
     7501 tagataataa caatctctta tcatctaaac tggcattatc aacttcatca ttaaatctct
     7561 tcaaattact aacaataata tcaatattaa tactgtcact actaatattg atgttactag
     7621 attccaaaat attaatctta tcctttaaaa tactaatctc attatttagg ctctcaatct
     7681 gggaaactat ataatcagaa gcagtactac cagtattttt tgcaagctct ataactaaat
     7741 ttttaataag tctttcctta tctaaaattt gatttttaag taaattagtc tcagatttaa
     7801 aaatattaaa aacattatca gtcttaatgt tattactatt gaatgcactt ataatagact
     7861 cttctttaca attcttaatt ttatctataa ctaaactttc aactaatgga ccattaaggt
     7921 ttttacagtt gcaactatca gacccaagtg tttttttagt accacaaata taataatact
     7981 ttaaatcctt gtcagttttt ttgcctttat aggtaattct catattagaa ccacatttac
     8041 cacattttat aagaccagat aaaagagcag tttttccagt tgatatcctt ggagttttat
     8101 cacgattttt atctaattga ttttgtactt caagccatat ttcaggttct atgactccat
     8161 ggtgttttgc aattgctgct attgagttag atgtattttt agcataagta aggattccac
     8221 attttctatc tggagtacct acaacatcca taccactatt tttaaggtaa tctaaaattt
     8281 catcattagc ttttacatat gctggatttc taagtattct agataaagaa ctagggtcaa
     8341 atttaccacc acgaggaccc cttattccat tattatagac atatttatat aacttagata
     8401 aggacctaag ttctaaatat ttattgtata ttagctttac aatctccatt tgttcatcat
     8461 ttacttgaag cttatacata cttctttctt ttagattttc atctaagaat gtgatttttt
     8521 cactgttgaa tccatgaggt gtattgcctc caagccatct accactacgg gctaattcat
     8581 acatattgtc ttttatacgt tctgctattg tttctctttc taattgagca aatacacttg
     8641 atataaacat catagctctt cccattggag ttgaagtatc aaattgttct ttgatagata
     8701 taaatgatat gtttagttca tttaattttt ctattaaagt agagaagtca gatacatttc
     8761 tcgatatcct atctaatcta taacaaacta ggtaactaaa tttcttatct ttggcatcct
     8821 gcatcatttt cttaaattgt ggtctatcta tattgctacc actaaatccc tcatcttcat
     8881 atatcaaaaa atcttttaca ccaatagact ttgcataatc aatacataat tgtatttgat
     8941 tttctataga atcacctttt ccagtaaatc ttgattttct tgaataaatt gcagctttca
     9001 aaattaaaac ctcctataag ttaggatata tatttgaaac tatttatcta caacctttta
     9061 agtaaactta ttagaatgaa tttattaatt aggtaaattt atataattct actttttata
     9121 atatatgact atttatttta tttctttact attcaagttc taaatcaata ataaattttg
     9181 atatttaatt gtatcaggac aaatctctaa tttcatagat tattaatata aaatcatagg
     9241 ttagtgtgaa aaagttgaaa tttaaatatt ttaaaagata atttttttga actaataggt
     9301 ataacaaaaa tttatagggg gattgcatat ggaaatcaat gttatagaaa ttttccctaa
     9361 agataaagct aaacttaata aaatagaaat ggataaagct agttggtttg taaatataat
     9421 aagtaaaaaa tatcctaaag aagctttaaa tgaagcattt agtactttag aaaaagaatt
     9481 aaatataagt aaagctaata cataagcttt gattattgat tttatatact attggtttta
     9541 tacgctatga cttgacatta tattaatcaa atagtatatt tttaaatata gcacaatgaa
     9601 gtgttaaaag agcaatggag ctccatagat aaagtctatg gagctttttt gcgtatttaa
     9661 cttaaggagg ataggacttt gaataacttt gagattaaaa ctagaataaa atttggagaa
     9721 ggctcactaa aagctttgca agaattaaaa aacaaaaatg tactaataat aacagaccct
     9781 tttatggtga aatcaaaaac aatagataaa ataacatcta atttaataaa ctcaacatat
     9841 caagtttttg aagaaatagt accagaccca ccaatagaag tagtagtagc aggaatagaa
     9901 gcatttaaat ctataaatcc agacgtaata atagcattag gtggtggttc tgcaatagat
     9961 gcagcaaaat ctataatgga cttttctaaa aaaatattaa aaattaacga agtagagttt
    10021 atagcaatac caacaacaag tggaacaggt tcagaagtaa catcatttgc tgtaataaca
    10081 gatagtcaaa aaaaatgtaa gtatccatta gtgagtgatg atttacttcc agatatggca
    10141 atactagacc cagaacttgt aaaaactgta ccaaatttta taacagcaga tacagggatg
    10201 gatgtattga cacatgctat agaagcttac gtatctacaa attctaatga cttttcagat
    10261 gcactagctg aaaaagctat aaagttagta tttaaatact taataaaagc atataaagat
    10321 ggaaatgaca tagaagctag agaaaaaatg cacaatgcat cttgtttagc aggtatggca
    10381 tttaaccaag catcacttgg cataaatcat tctattgcac atgtattagg tggaaaattc
    10441 catataccac atggaagaac aaactctata ttacttcctc atgtaataga atataattca
    10501 tcaatagtag gatatactaa cacaacttac tctgctacag ctaaaaaata tgcacaaata
    10561 gcagaattag taggacttgg gagtgctaat ataagaatat ctgttagaaa tttagtaaat
    10621 gaaataaaga aactacaaaa agaaatgaat atgccaacta aattgacaca atgcaaatta
    10681 agtttagaag atataactaa tgaagaaaga gaaatagcaa aacttgctat aaaagatact
    10741 tgtacattaa ctaatcctaa ggtaccaact gaagatgata taataaacat attaaagaac
    10801 attaaataac tacattttat tacatgtaaa tataatatta tacaaatagg gaaaatgttt
    10861 ttaaaaatat aaattatata ttgacaaata taaaacataa aactataatg aaagtatcac
    10921 aaaggcgtga tactttagta gaaagcaatg gagcttctta ggaatattaa tattattctt
    10981 atggagctcc ttttatttta atcaaatagc tttttaataa actatattca aagaaagcag
    11041 ggaataaatt tgactgaaga atctaagcaa agagttattc aagaatatgt tcctggaaaa
    11101 caggttacat tagcacatat catagctaac cctaatgaag atatatataa gaagttagga
    11161 ttagtacttg ataaaaaaga tgcaatagga atacttacaa taacaccaag tgaagcgtca
    11221 ataatagcag cagatgttgc tacaaaggct tcaaatgtat ctttaggttt tatagataga
    11281 tttagtggtt cagtagtaat atcaggtgat gtaagttctg tagaatcagc gttaaatgat
    11341 gttttagaag tcttaggaaa tatgttaaac ttctcatcta caaaaatcac aaggacataa
    11401 taatacaatg aagaatataa tatttatggg aaagacaggt agtggtaaaa ctaccttatg
    11461 tcaaaaacta gaccagttag aattaaagta taaaaaaact caatcagtgg aactttataa
    11521 taatgcaata gacactcctg gtgagtatat ggaaaataga aacctatata atgccttaat
    11581 aacaacatca gcagatgcta aaattatagc aatagtgtat gatgccacac aagaagaaaa
    11641 ttatatagca ccaggttttg ccagtatgtt ttgcaaagac gtaataggaa ttataactaa
    11701 aataaataaa gctactgaaa acgaagtcaa tatagcttat gaaagactat cactagcagg
    11761 agcaagtcaa atctttaaaa tagacacagt tgataatatt ggacttgaaa aattatttaa
    11821 atatctagaa aaatataaaa atatagatga agtttaaggg gaaatatttt tttattcaaa
    11881 aatattatta aagtatgata aaataaaaaa atgtataaac tattttaaga agatttaatc
    11941 tttaagcgag gtaggaacat ggggaaaaaa atactattgg ttgatgatga accactgtta
    12001 aggatggata caaaagatat attagaaagt catggctatg aaataatagg cgaagcaagt
    12061 gatggatttg aagctgttga aatgtgtaaa aaacacaaac ctgatttagt tataatggat
    12121 atagagatgc caatacttga tggtataaag gcaggcaaaa taatatgtaa agaaaatcta
    12181 gctggtggag tacttttatt aacttcattt gaagataaag aatacgtaga aaaggctaaa
    12241 agtataggtg cttatggata tttagtaaaa acagcaagtg aaaaatcact tatcccaacc
    12301 atagaaatgt gtttaaataa ggtaaatgaa tttgaaaaaa tgaaaaaaga tttagaaaaa
    12361 gtaaattcta aattgaatga aagaaaatta gtagaaagag caaaaggtat acttataaga
    12421 gagtttaaga tttctgagga tgaagcatac actagaataa gaaaattaag tatggataaa
    12481 agaactacaa tgatggaaat tgccaaaatg attataatag gatacgatga ctaatacgat
    12541 aaaaaaaata tcaactgcac aaacatactt aaataataac gaaattgaaa aaattataga
    12601 agtatcaaaa tccttagatt taatggcttc tttttatgaa gctgatgttt ttatagatgt
    12661 actaagtaaa tttgatgatg aagcaattgt tgtggcacat ggtagaccta aagaaaagtc
    12721 aatgtactgt gaaaatgtta taggtaaaaa ggctttaatt gaaaatgaac caggagttat
    12781 aaagtgctta aaaacaggag aaagagtaag agatattaaa gctttaacac aggaatataa
    12841 actggtcaaa caaaatattc agccaataac actagatgac aaagttttag cagtactaat
    12901 agttgaaaaa gatataagta gtgaagttaa gaatgagttt aatgctagtg aagacgaaga
    12961 aaataaaaat ttacatcagt ttataaaact tatcggaaat aatgactttt tagttgataa
    13021 tttaaattca gccatattaa tatttgataa aaatggaaaa ttaaaaatta aaaatcgtat
    13081 tgcaactgag atatacaaaa atcttgggtt tggatatgat atacaagata tgcattacaa
    13141 tgatttagat atagaaacca acacctttga aaatatatta atggatgaaa attatgatga
    13201 gacaaatgaa gtttcaatag gtaatatgtt ttttaaaata aagaccatat acatcaaaga
    13261 tgatgaattt aaggtttgca aaatagtaca agatataagt gatttaaaga gaaaagaaga
    13321 agaattagtt ttgaaaacag tggctataag agaagctcat catagagtaa aaaataatct
    13381 tcatacagta atatctataa taaaaaaaca aagtagatta tctcaaaatg atgaagtaaa
    13441 gcagtgttta gataacataa taaatagggt atttgcaata ctttcgactc attatttact
    13501 atctaaagaa gttgataata atatatctat acgagaaggt atcaatttat tagtgtcaaa
    13561 tattcaaggt ggctatttat ttagtaagga tatagatata cgtataactg gtgaagattt
    13621 taaaattagt ggagaaaaag caacagcaat acttttggtt ataaatgaaa tactacaaaa
    13681 ttgttatgac catgcatttt cggatagaga acatggtact atacaaatat taataaataa
    13741 agataacgac tatagaaata ttgctattat agatgatgga attggatttg atagtaataa
    13801 tataaataaa aatagcttag ggacatatat aatagatagt tatataacac agatgttaaa
    13861 aggaaatata gagagaaaaa gtagtgagaa tggtacagaa gtattaatta ctatacctac
    13921 acaaattaat atttaaataa taactcatct acaaagtagt agttttaatt gagcaaagag
    13981 gcttaaggtt attaatagat taagttaatt gaatgtattt ataaaaatta taagtatata
    14041 ttcaatttaa caggtattta ttataacttt aagcctcttt gcttgtttaa gacatactta
    14101 aattttagga ggtgagaaaa tgagagaaga gttattaagt gttggtattg acattggaac
    14161 aagtactact cagttaattt tttctaaatt aatagtagaa aatactgaat caaatttttc
    14221 ggtacctaga ataagtatag tagacaaaga aattatttat aaaagcaaag tttatttcac
    14281 ccctcttatt tcaaataatg aaataaatgg tcaacaaata aaagatatag ttgaaaatga
    14341 atataaaaaa gcaaatatat ctaaagaaga tatacataca ggagcagtaa taataactgg
    14401 tgaaacagct agaaaagaaa atgcaaatga tgtacttcat actttgagtg gatttgctgg
    14461 ggacttcgtt gttgcaacag caggacctga tttagaaagt ataatatcag gaaaaggagc
    14521 tggagctcat atttattcaa aagaacattc aactacaatt gtaaatatag acataggtgg
    14581 gggaactagt aacatagcct catttaaaag gggagaagta accgatactg gatgtttgga
    14641 tataggtgga aggcttgtaa agatagataa aataagtaaa aaaataactt atatatcacc
    14701 taaaatagat aaaatcataa aagataataa tttaaatctt caagtaggca caatggctac
    14761 tgtagaaaat acaaagccaa tagtggatat aatggttgac ttattaatgg aaagcatagg
    14821 attaaagaaa cagacacctt tgtttgattt tataataacc aataaatcaa taagtataga
    14881 tgaaactatt aaaaatgttt cattctctgg tggagtagct gattatattt actatgatgg
    14941 agaaataaaa gattattttg aatatggtga cttgggaatt ttacttgggc aagcaataaa
    15001 aaattgtgaa taccttaaaa aagtaaatgt agttaaatct atggaaacaa taagagccac
    15061 agtggttgga gcaggttctc atactactga agtaagtgga agtacaataa cttacactag
    15121 cgaaaagttt ccacttaaga acttgccagt tttaaaatta tcaattgatg aagaaacagg
    15181 tacttcaaaa caaatcagtg aagctataaa aaataaactt aattggttta aactagaaaa
    15241 tgatttacag aaagttgcta tagccatgat aggaaagaaa aatccaacct ttttagatat
    15301 acaagagtat gctaaagggt ttgtagatgg aatgcaacaa atcatagaaa atgatttacc
    15361 ttttatagta gttgttgaaa atgatatggc aaaagtgtta ggtcaagcaa tatatgcatt
    15421 attaaattat gaaaaagaaa ttgtttgtat agacagtgtc aaggttgaaa atggagacta
    15481 tatcgacatt ggaaaaccta tagttgaagg taaagtttta cctgtggtaa taaaaacatt
    15541 agtttttaat taatagaagg aggtaagtaa atgattttaa atacaaaatt atttggacgt
    15601 acttatcagt ttaaaggctt atatgatgta atggctaaag ctaatgaaga aaaatcagga
    15661 gataaattag caggtcttgc agcagaatca gctgaagaaa gaatagcagc aaaagttgtt
    15721 ttatcacaca taaaattgga ggatataaga aataatcctg cagtggcata tgaagaagat
    15781 gaagtaacaa gaataataca agatgctgta aatgaaaaag tttatgaaga aataaaaaat
    15841 tggacagtct ctgaacttag agaatggcta ttggattcaa atactacaag tcttgatata
    15901 agaagaatat caagaggatt aacttctgaa atgatagcag cagttgcaaa attaatgtca
    15961 aatatggatt tagtttatgc agctaaaaag ataaaggtta ctgctcactg taatacaact
    16021 ataggagaac aaggaacatt atcagcaaga cttcaaccta atcatccaac agacgaccca
    16081 gatggaatac ttgcatcact tcttgaaggt ttaacttttg gtataggaga tgcagtactt
    16141 ggattaaatc cagttgatga ctctgttgag agtgttacta aagtattaaa aagatttgaa
    16201 gaagtaaaac aaaaatatca aataccaact caaacatgtg tattggcaca cgttactaca
    16261 caaatggaag caataagaaa tggtgctcct gcagatttaa tattccaatc tatagcaggt
    16321 tctgaaaaag gaaatgaagc atttggattt aatggcgaca ctatagaaga agctagacag
    16381 ttagctttaa aacacggtac agctacagga cctaacgtta tgtattttga aactggacaa
    16441 ggttcagaat tatcttcaga agcacatcat ggaatagacc aagttactat ggaagctaga
    16501 tgctatggat ttgctaaaaa gtatgaacca ttcttagtaa atacagttgt tgggtttata
    16561 ggacctgagt acttatatga tgctagagaa gttgtaagag caggacttga agaccacttt
    16621 atgggtaaac tacatggttt accaatgggt gttgatgttt gttacactaa ccatatgaag
    16681 gctgaccaaa atgatatgga aaacttagcc atactattaa caacagcagg atgtacgtat
    16741 tttatgggaa tacctcatgg agatgatgtt atgcttaact atcaaacaac aggataccat
    16801 gaaacagcat cacttagaga aatgtttgga ctaacagcaa taaaagaatt tgatgcttgg
    16861 atggaaaaga tgggattctc taaagatggt aagttaacag agttggcggg agacgcatct
    16921 gtattattag gataggaggt atttaatatg aatgagaagg atttaaaagc attggtagaa
    16981 caattagttg gacaaatggt tggagaatta gatactaatg ttgtgtctga gactgttaaa
    17041 aaggcaactg aagttgtagt agacaacaat gcttgtatag atgatataac agaagttgat
    17101 ataagaaagc aattactagt aaaaaatcct aaggatgcag aagcttattt agatatgaaa
    17161 gcaaaaacac ctgcaagatt aggtatagga agagctggta ctagatataa aacagaaaca
    17221 gttttaagat ttagagcaga ccatgctgct gcacaagatg ctgtattctc ttatgtagat
    17281 gaagaattta taaaagaaaa taatatgttt gcagtggaaa ctttatgtaa agataaagat
    17341 gaatacttaa caagaccaga tttgggaaga aaattttctc cagaaactat aaataatata
    17401 aaatctaaat ttggtacaaa tcaaaaggtt ttaatattag ttggagatgg gcttagctca
    17461 gctgcaatag aggcaaattt aaaagactgt gtgccagcta taaaacaggg tctaaaaatg
    17521 tatggtatag attcaagtga aattttattt gttaaacatt gtagagttgg tgctatggac
    17581 cacttaggtg aagaattagg ttgtgaagtt atatgtatgt tggttggaga aagaccagga
    17641 ttagttactg ctgaatcaat gtcagcatat atagcttata agccatatat aggaatggca
    17701 gaagcaaaaa gaacagttat atcaaatatt cacaaaggtg gaacaactgc agttgaagca
    17761 ggtgctcata tagctgaact tataaaaact atgttagata aaaaagcttc aggaatagac
    17821 cttaaataaa gtcgagggag tgtagaatat gaaaaatgat gtaattcgtc ctaatatact
    17881 aggaattaaa ataatttcaa atataagtcc agaaatggca caaaaactag aattaaaatc
    17941 acatcacaaa agcttaggtt taataactgc agactgtgat gatgttacgt atacagcact
    18001 tgacgaagca actaaagctt cagaagttga tgttgtatat gcaaaatcaa tgtatgcagg
    18061 tgctgcaaat gcttcaacaa aacttgctgg tgaagtatta ggaataatag caggtccaag
    18121 cccagcagaa gtaagaagtg gtctaaatgc agtagttgat tttctagagt atggagcaac
    18181 ttttataagt gcaaatgatg atgattcaat agcatactat gctcactgcg tatcaagaac
    18241 aggtacatac ctttcagagg tagcaggaat aagagaaggt gaagcattag cttatttagt
    18301 agcaccacca cttgaagcaa tgtatgcact agatgcagca atgaaagctg cagatgttaa
    18361 gatgtgtgaa ttatttgcac ctccaactga aacaaacttt ggtggagcat tacttacagg
    18421 ttctcaatcg gcttgtaaag cagcatgtga tgcatttgca gaagcagtaa aatcagttgc
    18481 agacaatcca acaggatttt aaggatagtt agatggatgc tataggctta atagaagtaa
    18541 taggatatgt tgctgcaata gaagcttctg atgcttgttt aaaatctgca aatgttaata
    18601 ttgtaagagt tgataaggtt ggagcaggta ttgttacttt aacaatatgt ggagatgtcg
    18661 gtgctgtaaa agctgcttta gaagcaggtg aaatagcagc ttccagagtt ggtacattaa
    18721 gaacatcaca tattataccg agaatccata atgaagtaac aaatgcttta ttcaaaacta
    18781 aagaacctaa agtctgtgaa gtttcagaag ataaagaaaa aaactttgag attacattgg
    18841 aaatagctga taatgtatcg aaaaatttat cagaaaaaga aattgtaatt ttagatgtca
    18901 atgaaaacag tggagatact gaaaatttat atagtgatat agataaaaca aacaaaaaaa
    18961 ttgaaacagt tgatgatagt ttaacagaag ctaatgagga aaaaatagaa aacactaatg
    19021 ataaagaagt attggaacaa gttaaaatag aaaattccga ttttgataga gatgacctat
    19081 caaagaacag tgtaaaaaaa cttaaagcta tggctaagaa attagattct tcaataactt
    19141 ataaagactt aaatgttctt aaaaaagaag aattaataaa cttagttaaa aagcttagca
    19201 gaagggatag atagttatga cacttttaga taaagattta gtatctatac aagaagtaag
    19261 aactcttgta agaagatcta aagtggctca agagaaactt tctaaaatga gtcaagagca
    19321 aatagataaa atagtaaaag ctgttgtaga tgcagcattt aataatgcta ctaaattagc
    19381 taaaatggca aatgaagaaa ctggatttgg aaaatgggaa gataaagtaa ttaaaaatac
    19441 ttttgcatct atgggagttt atgaaacttt aaagaacaca aaaactattg gaataattgg
    19501 tgaagataaa gaagccaata cgatagatat agctacacca gttggagttg ttgcaggtct
    19561 tataccttca acaaacccaa catcaacagt aatatacaaa actataatat ctctaaaagc
    19621 aggaaatact atagttttct ctccacatcc aaatgcaaaa aactgcatac ttgaaacagt
    19681 taatataata agtgatgctg ctataaaggc tggagctcca gaggattcaa tattatgtat
    19741 aactaatcca agtatagttg gaaccaatga attgatgaaa cataaagata caaacttaat
    19801 actagcaact ggaggagaag caatggttag agctgcttac tcttcaggaa caccagctat
    19861 tggagttgga ccaggaaatg gaccagcatt tatagataaa tcagcaaatg ttaaattagc
    19921 aatcaaaaga ataatagatt ctaaaacttt tgataatggt gttatatgtg catcagaaca
    19981 atctatagta atagaaaaat ctatggaaga aactgtttta aaagagctta aaaatcaagg
    20041 tgcatatgtt ttaaatgaag aggaaagtga aagattagca aaattcatat taagagctaa
    20101 tggtactatg aatccacaaa tagttggtaa aagtgttgag gtaatagcaa atttagcaga
    20161 cttaacaaat gtacctaaaa atacaaaagt tctagttgca agagaaaata aagttggtca
    20221 caatgtagcc tattcaagag aaaaacttac accaatacta gcgttctatg ttgaagaaaa
    20281 tgtagaagct attatgaaca gatgcagaga aatattgtta aatgaaggtg caggtcatac
    20341 attctcaatg catgtaaatg atgaagaatt ggtaaaaaga tttgccctta atatgccagt
    20401 atcaagaata cttgtgaata ctccttcagc acttggagga ataggtgcat caacaaacct
    20461 tgtaccagca ctaacacttg gttgtggtgc aataggtgga agttcaactt caaacaatat
    20521 aggaccactt gatttaataa atgttaaaag agtagcttat ggaatcaaag aaatagaaga
    20581 acttggaaga gataaatctc aagttgaaaa atcaatgttt gactcaatgg ataaagaaga
    20641 attaataaat cttcttgttc aaaaactagt caaagaacta gcataactaa aataaacaat
    20701 gaaacttata gctatcgcta taaatttcta aaaatataaa ctataaaaaa ctataaaatt
    20761 aaatttttgg aggataagat tatggcaagt gcaaacgcat taggaatgat agaaactaaa
    20821 ggattagtag gagcaataga agcagcagat gcaatggtta aagctgcaaa tgtacaatta
    20881 gtaggaaaag agcaagtagg tggaggatta gttactgtaa tggtaagagg agatgttgga
    20941 gctgttaaag ctgctacaga tgcaggagct gcagctgccg agagagttgg agaactaata
    21001 tcagttcatg ttataccaag accacacttt gaagtagatg ctatattacc aaaggtatca
    21061 gctgagtaat ataagaataa ttataaaatt taaattttga ttgaaaaaga ggctatctca
    21121 aaaatgagtc ttactaataa actcaatgta ttttgaggta gtctttttta tacatctaat
    21181 aggagatgtg ataaaatggg tgttttaaca gaaaacgaag taaaaagaaa aattggaaaa
    21241 aacaaaaata atataactga attttatata gaaaaaggtc aaataataac tccttctgca
    21301 aaatcttatt tagctgagag aagtattgag cttaaatatg ttgataaact ttcagatgta
    21361 aacaatgaac ctaaagaagt tgtaaaagaa gtagttaaag agaaaaaata taaatatact
    21421 actgtatttg gcgccaaatt ggatgaaaaa ccagaacata tgactcaatt aaatggaaat
    21481 cttttagttt ttaaagacca taaaagaata atattgagag gaaaaataga ttctcttgaa
    21541 tcagaaatac taaaggtaca gatactttgt caaaaactag atatgccaaa gcttgttaag
    21601 gaattgcaag aagtattaga ctttgtaaga aatataatga gatgtgaagt tttagatgaa
    21661 aagctagggg actttaaact tttaggatta actcctcaag aacttagaga acaatctcac
    21721 tatcctaaaa aatactttgg tataggacat gaattcccaa gttatgaaat gggagaagta
    21781 gtagtagcta taaatagtat aagaagtaat acacgagagg ttgaacttgc agcctatgaa
    21841 gcatttaagg gtgagtatgg aaatgttcac agagaagatt taataaaggc acttaataga
    21901 ttatcgtcag tattttggat aatgatatat aaggttagaa ctaataagta taagtagttg
    21961 gaggacataa tggagaatct attaaatgaa atagtggaag aagtaataac tagagttaaa
    22021 aaagaagcat ttatagaagt tgaagcatca ggaagacacg ttcacttaag tagagaatat
    22081 gtcgataaat tatttggaga aggatatact cttacaaaac taaaagacct atctcaacca
    22141 ggtcaatatg catgtaagga aagagttaca ataactggtc ctaaaggaag tataaaaaat
    22201 gtagttgtac ttggaccatg tagaaatgaa actcaggtag aaatatcttt aacagatggt
    22261 tcaactcttg gactaaaagc tcctataaaa caaagtggag acttagaagg tactttaagt
    22321 attaaaatat caacacaata tggtgaagtt gaacttgata aggggctaat ggttgctaaa
    22381 agacatatac acatgactcc taaagatgct gaaaaattca atgtatgtga taaagagata
    22441 gttgaagcta aggtgatggg tcaaagacct ttaacatttg atgatgtagt aataagagtg
    22501 agtgacagtt ttaaaactta tatgcatata gactatgatg aagccaatgc atgtggttat
    22561 tcaaaaggaa ctgttgctaa aataataaag aaagcataaa tttaacctac tatataaatt
    22621 acctctaggg aggatttaat atgaattatg ataaccttgt agatttaata acggaagaaa
    22681 tctataaaaa aataaatagt aatgaaataa aaatatctaa taaacctaaa gctgtgattg
    22741 tatttgaaca agataacaat aaatttaact tattaaaaga tgaatttgaa atagtagaat
    22801 ttgataaaag tataagggaa tgtgaaatag tgatagtttc aagactttgt atgagggggc
    22861 tatcgaatat agcacttgga aattcaacaa gtgatgaaga aagatttata ctaaaaatga
    22921 ttatgaaggg caaaaaagta tatgtcctag atgaaggaat tgaatacaaa aaatataagg
    22981 atactgcacc aaaagcatta tataagaaat ttatgtcatt tgaagatgaa atatgtaaat
    23041 ttggtgtaga aattataaaa gacttaaatg ttataactaa aaataagtta aatataaaga
    23101 aagaactaga aagtactaga ggttctaaaa aagaagatac aaaagtttta gataaagatg
    23161 ttttaaattt aaccagtgta gaaagtgtaa aaatagataa tgaatttagt ttagatttaa
    23221 ggaataaaaa acttatatcg gaagctgact taagaaaacc aactataaat ggagttaaaa
    23281 acatattagt taataaaaaa agtataataa caccattagc tgtagatttt atgagaatac
    23341 accatctcaa actaaagaaa ctgtaaggag gtaattttat ggatatagga aaagttgtag
    23401 gtaatgtttg ggctacaaga aaagatgaaa aattatgtgg ccaaaaactt ttagttgtaa
    23461 aaattttaaa aactaaagac acatacaagg aaggcttcat ggttgcagcc gataatgcag
    23521 gagcaggaaa tggagactta gttcttatat ccaaaggttc ctcagctaga gagtcgattg
    23581 aaaaatctca tgttccagta gatgcaacta tagttggagt agtcgattcg tttgaggtgt
    23641 ttgatgaata aaagtatagg agctatagag tttaaaagca ttgctaaagg tatagaagta
    23701 tccaatgaaa tgataaagaa atcttcagtt gatgtattgt acttaaaaag tatttgccca
    23761 ggtaaatttc taataatagt aggtggagaa acttcttata taaatgaatg tgtagattat
    23821 ggaataaaac ttggagaagg atatattgta gataacttcg taataaatgc aatttcacaa
    23881 gaaatattag atggatttaa aaataagtat caaaagctag atagtatagt ctctataggc
    23941 gttgttgaaa gtagtaaaat atgcgctgga ataaaaatgc ttgataagac tttaaaatct
    24001 ggcgacttag tattagtaaa acttcaatta tcttttgcta taggtggcaa attagtatat
    24061 atagtagcag gagatttaag tagtttagaa tatgcattaa aagaaagtga aaatgtagta
    24121 agagaaaaag aagtaatata taagactgta ataccatcag tagacagtca aataataaaa
    24181 agtctaataa aataatgggg ggaattatga gtataaacga aattataatc tatgtaatgg
    24241 tagtcttcat ggtacttgga gctatagata aatgtatagg taacaaattt ggtcttggag
    24301 aacaatttga agaaggtata atggcaatgg ggtcacttgc agttgcaatg gttggtgtta
    24361 tatgtttagc accagttctt gctgatgtat taagaccaat tgtagtacca gtatttaata
    24421 tacttggagc tgacccagct atgtttgctg gaagtttatt agctaatgat atgggtggtg
    24481 cgccacttgc acttgaactt gcgaaagacc ctaatgctgg gttatttggt ggattaatag
    24541 taggtgctat gatgggacct acaatagtat ttataatacc agttgcgctt ggaataattg
    24601 aaaaagaaga ccaaaaattc ttagcaacag gaatacttgc aggtataata acaataccta
    24661 ttggagcatt cgttggtgga ttagttgcag gattcgaggt aatgatggtt cttagaaact
    24721 tagtgccaat aataatattt gcagtagtta tagcacttgg attattaaaa ttcgaaaatg
    24781 ctatgataaa aggattcaca tactttggaa aaggtgtagt aatagttata actttaggac
    24841 ttgctgctgc catagttgaa gctttaacag gaatagttgt aatacctgga atgaaaccaa
    24901 tcagtgaagg tatagaaata gttggagata tagcaatagt tttagcagga gcattcccat
    24961 tagtgtttgt tataacaaag gtatttaata aaccattaat ggctttagga aaagttcttg
    25021 gaatgaatga tgtatcagca gcaggccttg ttgcaagttt agctaactgt ataccaatgt
    25081 ttggtatgat gaaagatatg gacaatagag gaaaaataat aaatgtagca ttctctgtat
    25141 cagcagcatt cgtatttgga gaccacttag gatttacggc aggatttaat tctaatatga
    25201 taacaccaat gatagttgct aaattagttg gtggtataac agcagtattg ttagctatgg
    25261 ttatcgcaaa taagacacta aagaaggaga atgtataaga tggatatatc aaatatagat
    25321 aaaaatttaa ttgaaacatt agtaagacaa ataatagaag aaaaaataag tggaactaaa
    25381 gacacagtag attttgttag aaataaagac attagtggaa taacttctat aaagttacca
    25441 actgtaaaag ttagtgaatc tgatagatta gatacaggga atccaagtga tgtagtttac
    25501 acaaaagatt tatttacatt agaggaaagc ccaagattag gttgtggtat gatggaaatg
    25561 aaagaaacaa cttttgattg gactctaaac tatgatgaaa tagattatgt aatagatgga
    25621 actttagata taataataga tggaagaaag gtttctgctt cttcaggtga attaatattc
    25681 ataccaaaag gaagtaagat acaattttct gtacctgatt atgctagatt tatatatgta
    25741 acatatccgg cagattgggc atctcaaaat taataaaaat caagttaagt taataataat
    25801 caaataaata atttaaaata caaactctta aataagcaca catatttaaa aaagctatga
    25861 attaaaattc atagcttttt ttataaataa tgttatattt tagacgtatt ttttctatat
    25921 ttactgggag acatattata ttttttatta aataatctaa taaaatgctc tacatttgga
    25981 taaccaatac tattttactg ataatctttt ccttatcact aatcttacat tttaaaatat
    26041 tatttttact cgtaatatta tcataagaat ttgtttctta tttaaaacta attagaatta
    26101 taaagtttat tgccatgtta ttaataaaat aaatccagtt aaatcgagtg ctagttaaag
    26161 taataagaaa cttttacaat ggtttccttt atcgattgaa ataaaagtta atatggtata
    26221 atatctaaag agagaatctt atcaattaga atatgcatag aagcaagtgg cttaaaagga
    26281 gacgttatat ggaagagata gtaaagaagg aacaagcaag tatgaatgat atttttttgt
    26341 atcttaaagc taaatcaaac tcattatata aatttgtaat attatataat gagtttatca
    26401 ataaacctaa agattatgga actggggaac ttataaacat ggttgaagta catatgctaa
    26461 cagcgattga agaaaatcca ggaataagtc caactcagtt agctaaaata tggaacagaa
    26521 ctaaaggtgc aatatctcaa actatatcta aattggagaa aaagggttat ataactaaag
    26581 aaaagaaaga aggaaatgca aaaactatat tactatatcc aaccacatca ggagtagagt
    26641 taagtaaggc acataaacta tatgacacca tagatgttac aagaacattt gaatacttaa
    26701 tcaaggagtg tacattagag gagatagata gtttttataa agtaattgat ttgtttatta
    26761 atttattaga agaatctccg caggaaaata atgattctga aaaagtgtag ctaagattga
    26821 agttgaataa agagaaatag atattataga agtagtactt aagtacacat tatataaaaa
    26881 tataataggt atatgattaa aaagtttagt ggtttaaagt ttaaccacta aactttttta
    26941 atgtctataa tctactatta tttaatttac taaaaataaa agaactcatt atattaagat
    27001 aaaaatagaa aaataactag aaaaatacta taatatatca gcttaataca gaaaaaagtt
    27061 aaaaaatttg agaaaaataa aaatgaaaac gttttatatt gacatagaga aaaagtagtg
    27121 ataccattta aatatagttt agcagctaaa ctatatttaa attataataa ctattttagg
    27181 gggatgttat gaatttaaaa caattaagtg aaaaatacga agaatatgta attcaaatga
    27241 gaagagaatt tcataagtat ccagaattat cttttaaaga aataagaaca acccaaagaa
    27301 taagggaaga attacaaaaa ataggggtcc cttttgtatc tattgaacca aatatcgtaa
    27361 tagctactat acaaggcaaa aatgaaggta agacaattgc aattagagga gatatagatg
    27421 cgcttccaat gcaagaagat tcagatgttg aatacaaatc agaatatgat ggaataatgc
    27481 attcttgtgg acatgatgct catggagcta tgttactagg acttgcacat atgctaaatg
    27541 atataagaaa agatttaaaa ggtagagtat tattagtttt tcaagcagct gaagaagttg
    27601 gtggtggaca caaggagata atacagtact ttaaggaaaa tggaggacca gatagattaa
    27661 tagctactca tgtttggtcc gatattgaag ctggtaaaat ttctgttgaa ccaggacctc
    27721 gtatggctgg tgggtctggt tggagaatag acataacagg tagaggaggt catggttcta
    27781 gaccagatca aagcatagat cctataaagc cagcttgtga tatagtttta aaagtatctt
    27841 ctataccagt taatcatata agtgtactag aacaatgtgt tgtacatgca tgtgcaatac
    27901 atggaggaac tacaatagga aatgttattc caaacaatgc agaggtaata ggtggatata
    27961 gatattttac tgaagaatca ggcaaaaaag tgtttgacat tataaagcaa atgagtaatg
    28021 gtgttggaca agtttatgga gtggatgtga aggttactca tactggaggc ataggacctg
    28081 ttatcaatga tgaagaagca gttaagatgg ctaaagaaat agtttctaat atagatggat
    28141 tggagttaga ttcttatgag ccaatatgtg catcagattg ttatggtgaa atattaaaag
    28201 agtattctgg cttttattgt tatctagggg taggtaacaa agaaaaagga ataatatatg
    28261 cacaacatca tcctaagtac aatatagatg aagatacatt aaagttaggt tgcgaattta
    28321 tggctaaata tgcagttgaa tttttaaata aaaataataa ctaaaataaa tcgaatttta
    28381 taaatggggg gatgaaaatg caaggtgcat taaaggaaaa taagaaaaaa agtggtggag
    28441 gatttttagg atggattgaa agagttggga ataaaatgcc acatcctcta gcattattta
    28501 catatattac attgatagta gtagcattat ctgcaatagc aagcttttta ggtttttcag
    28561 caacaagtcc tgctacagga aagttaataa aagttatgaa cttaataagt gcaaaaggtt
    28621 tagtactatt tatgcaagag tttgttaaga acttccaaaa cttccctgtg cttggagtag
    28681 ttttagttat ggcaattgcc acaggagttt gtgaaaagac aggatttttt tcaactgcca
    28741 taaaaatgag tttatcaaaa gttaagggga atgcggttgt atttataata gcttttatag
    28801 gagtatttgc taatcaagct ggtgatgctg cttttgtatt agtaccaact cttgcaggag
    28861 caatatttta tggattaaat agaaatccat tagctggtat attctgtgga tttgcatcag
    28921 ttggtggtgg atttgcaaca gctgtaattc ctgggggatg ggacgttacg ctaacgccaa
    28981 taactgtatc agctgctcaa actattcaac ctgcatttga tatgacatta ttagctagtt
    29041 attatgcact atttgtatct gcgtttatag tagctacagt atcaacaata gtaacggtta
    29101 aatttataga acctaaatta ggtaagtata ctggtaaacc agaaggtata gatgataatc
    29161 aaggttttga ggtcacaaaa gaacaagcaa aagcagcaaa gttagcaggt tttacagttt
    29221 tagcttatgt agcgttatta attgctttgt gtataccagc taatagtttt ttaagaagtc
    29281 cagaaggttc tttaatattt ggagcaccac ttatgtcttg cttacagttt tttatagtaa
    29341 tactattctt cttgccaggg attgtgtatg gaatgagggt tggtaaaatc aagactgtaa
    29401 aagacttaaa tgatatatta tgtcaatcaa tggcagcaat ggcaccgttt atcgtacttg
    29461 caatagttat aggtcaattc ttaacgctat tctcagtgtc taatttagca caggtactag
    29521 ctataaaagg tggagcatta ctcaactcat tgccactacc accacaagtt ataatactgc
    29581 tattcttagt actagtagcg tttataaata tatttgttat aagcggaagt acaaaatgga
    29641 tgatatttgg accagtattt gttccaatgt taatgcaatt aaacatacat ccagcattta
    29701 ctcagtttgt atatagatta ggagattctg taaccaatca tttatcacca ttaaatgcat
    29761 tctttataat acttttagca acagtccaaa aatacgataa aaatgcaggt atgggaacta
    29821 tattctcagc tatgatacct tacactatag gttactttgt agtacttgca gttttagtag
    29881 tagtttggat gacaataggt ataccagtag gaataggtgg acaagtttgg ttataaaaca
    29941 tatttaataa ttttgggagg aaagaaaatg gaaacaactg aaataagata caattttgat
    30001 gaagtaatag atagaagtgg aacaaactgt cttaaatatg atcttaagag tcaatttatg
    30061 ccagaatctc cagaagatgc actaccacta tgggtagcag atatggattt tgcatgtgcc
    30121 cctgagataa ttaatgcaat gcatgaaaga atagacagaa aaatatttgg atatagctct
    30181 caacaaactg atgattatta tgaagctgta tgtggatggt ttaagagaag atttgattgg
    30241 aaaatagaaa aagagaatat attctttagc ccaggggttg tgcctgcgtt aggatttttc
    30301 acacaaatat taacacaaaa gggtgatggt atcataatag aaaagcctgt atactatcct
    30361 tttgaagata aaataactaa caatgaaaga aaagtagtta atagtccttt aaagtttgaa
    30421 gatggtcatt attctatgga ttatgatgga tttgaagaat tagctaaaga tgaaaataat
    30481 aaggtgttta tattatgtag tcctcataat ccagttggta gagtatggaa aaaagaagag
    30541 cttagaaaaa tagtagacat atgtaagaaa tatgatttgt ggataatatc tgatgaaata
    30601 cattgtgata ttattcgtaa aggtgaaaag catataccat tagaagtagc atgtcctgat
    30661 tataaagata gaataataac ttgtacagct ccaagtaaga gttttaactt agctggtatg
    30721 caattatcaa atatagtaat caataataag gaattacaaa aaaaatggat gcttgaaaca
    30781 gattctaaat tagctatatt cctacctaat ccattcgcaa tagttgctac taaagttgct
    30841 tataatgatt gtgaagattg gatgaatcaa gtaaatgagt atatagatgg taatattaaa
    30901 tatgtagaag attttgttaa tgagtatatg cctaaagtaa agataataaa atctcaagga
    30961 acttacttag tttggttaga ctttagaggg tatggattag atgcaaaaca gcttgaatat
    31021 gtaatgctta acaaggcgaa ggtattgcta gatgaaggat acatattcgg agaagaagga
    31081 gcaggatttg aaagaataaa tgttgcttca cctagaacaa ttatagaaga gtgtatgaat
    31141 agaataaaat gtgcatttga aggattataa tagtaatatc taaataatat atttgtgtat
    31201 tttaaaaaaa gactatgcta aaaaaatatt tcaaagcata gtcttttaaa gttttatgat
    31261 gagtttaaaa aatttcaaaa cgttactatt cacttatatg tttatattag aaataattcc
    31321 acaatatact cattttctaa agttgcactt ttatacacct tgcttcacca atatcatgtg
    31381 taaccaaaat agtagactta ttctcattct taataatact ataaacatca tcacaaacta
    31441 ataatctagt ctgataatca agagctgaaa aaggttcatc taaaagtaga atatctggat
    31501 ttacagaaag tgttctaata agcgcaactc tttgtctcat acctccagat aattcttttg
    31561 gatacatact tctaaaatcc cataatccat aagtctttaa aaggctttca actctatcta
    31621 aagatttttt atcttttttg ccttgaattt ccaatccaat agttatatta tccattatat
    31681 ttctccattc aagcaaatta tctttttgaa acatatatcc aatatttcca gtaatcttta
    31741 tatcaccact agtcggtttt aaaagattag taagaatatt taatattgta gatttaccac
    31801 ttccagaagg accaagcaaa gttaatattt ctccttcttt caagttaaaa ttgacatcct
    31861 ttaatacttc aagctcgcct tccttagtgt agaagttcat atttatattt ttaacttcta
    31921 ctatgttgtc attcattatg ccacccccta taacttttag ctcgtatata ttatgttatt
    31981 aacaatatga cctatttgta ccatttttca taaatataat ataaatattt tgtttattta
    32041 cgtattttct tagtaacttg tacgtataaa ttatagataa ataaattata aggtagggga
    32101 taaatttgag taacataata acgaatttaa tagagtcaat atttactaat ccaataacta
    32161 tattgttttt acttgcaata actgtgtatt ctgtttttaa aataatagaa aataacagag
    32221 tatattcatt tgtaagttca aaattagacg aaatcaatag agactataag acaagtgagt
    32281 tttataaaaa ggtaagggat gactactata catatagtaa agaaaatcct tatgcagacg
    32341 taaatataac ttcatttata gaggaagttg tatctgattt aaagcataat aatttgccat
    32401 tgttagaaaa aattagaagt ataaaaaatt catcttcaat aagtatatta cttggggttt
    32461 taggtacgtt tgttggtctt tctacaatgc ttttatgtgt tgatactaaa gacattataa
    32521 attcacttcc ttcaactatt agtagtatgc aaactgcatt tacaactagt atatttggag
    32581 ttgtattttc tctaattata ggttacttta ctaagataaa agattgtgaa catgttttga
    32641 tacaaataat gttaaaatct gaaaatttac taacatctga aataactcat tttaagtcag
    32701 aaagaatgga tttaaaagta gaagaggtaa aaaatacaat aaaacaaata agtaagtcta
    32761 tagaagctat tgaaaaattt gataaaatat ctaaagattt aaatgacttt aatgatgaat
    32821 ttatcagtgg tatagaagct ttaaaaagcc ttttagaagg ttctcaaagt tctattaaga
    32881 catttgacca gagcgtaaga aaattagata aacagtttaa tattttaaat ataaaatttg
    32941 taaagctttt tgataaatac gataatcaag ataatataaa taaagagatt ttatttgata
    33001 taaaagaatc ttcaaaaaat atatacaatg ccactgaaag ccaattcaaa attagagatt
    33061 atataaaaaa tataaatgca ggatttgctc tatatgaaag aagtgctcaa gatttattaa
    33121 ctaaattaat gacccatgaa aataaaatat ctcaaaatca aaaaatcctt ttagatgaaa
    33181 agtttacctt agatgactca ataaaaaatc tctcaagtat tattgaaaac ttttctaatg
    33241 acctacaagt taaactggat atgatgtttg aaaattcttt agatattcaa gataagttag
    33301 atgttatgtt taataactct tttatgaatg atgaagttcc tcttgatagt gaagaattat
    33361 ttaaggatga tgttaataat gtatttaatc cttttagtga agaaatttat gagatagagg
    33421 ataaagaaat aaaggtcata ggtgaggatg agttaaatga ataataaata tagaagaact
    33481 gttaacagag aatttgaaaa gagtagcttt tggccaacat ttacagattt attatctact
    33541 gtattgatgg ttgtaattct tatcttattt agctcagaaa gtatttcagg ttctgttgaa
    33601 caagatttag ctaagaatgt caatgcttct gttgaagaaa cttttaaaaa aagtggaata
    33661 cctgtgaagg tagataaatc taatgggcaa gtaacttttg gtgaaaaaac catgtttgat
    33721 gtagatagtg atgttttaaa accagaggct aaagaaatgc taaaaatgtt tgttccaaag
    33781 tacatagaga ctatttataa agattatggt gactatatat ccaaaatagt tatagaaggt
    33841 catacagatg atgtgggaag ttatatatac aacttagatt tatcgcaaag aagagcatat
    33901 agcgttgcta aatttatagt gggagatgaa ataggtgact ataaatacaa agataaagtg
    33961 actaagcata ttattgcaat aggaaggtca aaagctgaac tcatcaaaaa tggtgacaat
    34021 agcgtgaata gagatgcatc tagaagagta gaactaaaat acgaaataaa tattaatcaa
    34081 aataaataat taaattttta taaaaaaaaa gaaggcagtt ggtgtgagct ttaagccttc
    34141 tttaaggaaa aagtttattt atttttgttg atacttaaca tattatagtt tttagaagca
    34201 gtttgcaata tattaaacgt aaatggtc
//
LOCUS       DAELJO010000021        30827 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELJO010000021 DAELJO010000000
VERSION     DAELJO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.021"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            138..680
                     /locus_tag="KPB88_000906"
                     /pseudo
     CDS             138..680
                     /locus_tag="KPB88_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            812..1474
                     /locus_tag="KPB88_000907"
     CDS             812..1474
                     /locus_tag="KPB88_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053206.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1474..2655
                     /locus_tag="KPB88_000908"
     CDS             1474..2655
                     /locus_tag="KPB88_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053207.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKSTSWSKNFFYFLVVSFGI
                     ISTMAIVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKA
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMTQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEDSLREKHQTFTI
                     EGSPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2835..5495)
                     /locus_tag="KPB88_000909"
     CDS             complement(2835..5495)
                     /locus_tag="KPB88_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0053208.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     IYRNATSEYWNHMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPIEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLIQLGILSSIGALPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIQQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAKAVYGSDNYKDQRDIEVYLENSEPVE
                     SAFIEKLNNTEGVKSSLYYQKMSAATWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAIASTLPKIIDTSSRYTLMQIVPMS
                     MVQEISKNYDEVGRLRSNRLIGLFKVNNREDIPRVREKLESICNKDYGSGDYSIEDVL
                     ESEKQDASGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5492..6175)
                     /locus_tag="KPB88_000910"
     CDS             complement(5492..6175)
                     /locus_tag="KPB88_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053209.1"
                     /translation="MEILKCENLTKIYGSNQTKVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEGRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(6519..7457)
                     /locus_tag="KPB88_000911"
     CDS             complement(6519..7457)
                     /locus_tag="KPB88_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase CDD-1"
                     /protein_id="HBG0053210.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYAVKKHNDINQKTDKNYIKSEL
                     NKSKIKNNDKNKKESVNIVDYSDCFEGISGGAIFCNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTDYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(7998..10112)
                     /locus_tag="KPB88_000912"
     CDS             complement(7998..10112)
                     /locus_tag="KPB88_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0053211.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDTELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNHGEIDPHEMFQNEQGLKQIDNYGDFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKPIISTIQKEQNKIIRNDKADTLIIQGVAGSGKTSIVLHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     AERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNVMLTIKNTLTLYKDFFKQMNISN
                     MFVMAAKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHAHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKVQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKTEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLAYTGELTQLIDM"
     gene            complement(10362..11222)
                     /locus_tag="KPB88_000913"
     CDS             complement(10362..11222)
                     /locus_tag="KPB88_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053212.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDVVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLANMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFEEAELLVEFGYPTQKFILP
                     YVEERDNRQN"
     gene            complement(11432..12901)
                     /locus_tag="KPB88_000914"
     CDS             complement(11432..12901)
                     /locus_tag="KPB88_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0053213.1"
                     /translation="MELQETKFSFVYSEIKQRIMNNHIPPGDYLPSSRMLCNQFNVSR
                     YTINRVFDALKEEGLIEIKPRIAPIVLSRTVMPESKDILSDILRKRDDIVQIYQTYAL
                     LLPPLLVFASQNCNLEMLHHYKKTMKISRLGISAGGWRSTSIFIKEILEIGGNHLLSN
                     LYSTFELYSNLSFFIEECPYFLEKFLQKPVSFTRVIVDILRGNDLSIKYIQLVNLFQR
                     FSDSIADVLEKLSNTTPQYQTQYDISFSWKPTRGKDYFYSRIINDLNQKIGNGEYPRG
                     TYLPYEKQLANQYGVSVSTIRKALAELEQRGLIKTLNGKGTVVIEPDDSQVKQLLSNS
                     RRTEEALQYLHALQLLVLIIHPAALMAAPKFTSSELDELIEKLILPNSICIIDIFEAI
                     LKHIELEPLRVILTETFHLTEWGHYISYYDNKKRVLKNLNKQIIVAVKYLHEGDMKSF
                     ANDITDSYRYALKHAKEYMKKKYSFYSASCIQIPEKYDE"
     gene            13114..14754
                     /locus_tag="KPB88_000915"
     CDS             13114..14754
                     /locus_tag="KPB88_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0053214.1"
                     /translation="MKKQKILIVDDSEMNRSLLIDILENQYDIVEANNGAEAITILSQ
                     HRTDFSLLLLDIMMPEMDGFEVLAYIKKHHWDDTFAIIMISADNSTTNIKHAYDLGAF
                     DYIGRPFDSAIVHRRISNTMLLYARQHYLENIITKQFHEQEKNSKLMISILSHIVEFR
                     NGESSLHIQKVNIITNLLLRTLVQQTEQYFLSESDITLISTASSLHDIGKISIPNKIL
                     NKPGRLTVEEFDVIKKHSKIGAKMLQELSVEQQESSLVKVAYEICRWHHERYDGNGYP
                     DGLKGEEIPISAQVVALADVYDALTSERCYKKAYSHDKAVEMILNGKCGSFNPILLQC
                     LINISDILETKLKDVANVKPNTIDIRRMVEKRKNMIHERCTNYLTYEILYTDPLTNVY
                     NRRYYEDHILNLNEIQNITLIDVDNFKAINDSYGHYVGDIVLQEISKTISSCVRKIDT
                     VIRYGGDEFIIIFSYIPTNLFKKRLEIIRTSVNMIKFEEYPEICISVSIGGICETNKA
                     THLFKIADKLMYQAKTKRNKVIYVTDRNTVDINEREEV"
     gene            14756..15118
                     /locus_tag="KPB88_000916"
     CDS             14756..15118
                     /locus_tag="KPB88_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HBG0053215.1"
                     /translation="MNLSDCYIIFGGNYDEVLCRLQHEHIVQKFIFKFLDDKSFNLLK
                     VSIDNENYDDALRFAHTLKGICQNLSFSRLYESSNQITIELKDGNYKKAVDMIPQLSK
                     DYYKTIDIIKEYKMSKEK"
     gene            15124..17682
                     /locus_tag="KPB88_000917"
     CDS             15124..17682
                     /locus_tag="KPB88_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0053216.1"
                     /translation="MSSGKTKNHITRFLAYSFIGLILFSVAIFSILGIFMNSRSQKSF
                     YKVGEIYMSGMNEEMSKNFENVIKLRFNQVEGIISTVPEDSSNREKLYEELVYRAQIR
                     DFDYLAVCSEDGHFETLYGEAIQPKNPLPFVRALKQKNRRVAVGVDAIGNEVVLFGID
                     ANYPMQNGEEGIGLVAAVPLNYITEFLSLKDKGDMMYYHIIRPDGSFVIRNLNTELWE
                     YFDLLQKQQNQVTEESSLKNPLVGLDVALQDKKNYSTVVEVNGEYHQIHGTPLSYSEW
                     YLIAVMPYEMLGETMNSLSVDRMVATMLACASVLVFLIVIFLRFFVMTRLQLQELDKN
                     REMALEANKAKSEFLANMSHDIRTPMNAIVGMTAIAKAHIDDQEQVKNCLRKITLSSK
                     HLLGLINDVLDMSKIESGKLALTMEQISLREIIEGIVSIIQPQVKVKKQNFDIHINNI
                     LTENVWCDGVRLNQVLLNLLSNATKYTPEGGSIQLDLFEEKSPKGEDFVRVHIKVKDN
                     GIGMSPTFLKKIYESYSRADGTRIHKTEGAGLGMAITKYIVDAMEGTINVQSELDKGT
                     EFHIIVDFEKASMMEIDMVLPSWNMLIVDDDKLFCETAINTLKSIGIKAEWTLSGENA
                     IKLIIQHHKKRDDYQIILLDWKLPGMNGIQIAKEIRRNLGNEIPILLISAYDWSEFEA
                     EAREAGISGFISKPLFKSTLFYGLRPYMDIGTTQDQMLNNNIDMSGFRILLAEDNELN
                     WEIAREILSDLGVELDWAEDGQICLTKFQESSKGYYDVILMDIRMPHMTGYEATKAIR
                     TLDHPDASSIPIIAMTADAFSEDVKHCLGCGMNAHIAKPIDVTELTRLLKKYLM"
     gene            complement(17739..18121)
                     /locus_tag="KPB88_000918"
                     /pseudo
     CDS             complement(17739..18121)
                     /locus_tag="KPB88_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362366.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18132..18494)
                     /locus_tag="KPB88_000919"
     CDS             complement(18132..18494)
                     /locus_tag="KPB88_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0053217.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(18724..19188)
                     /locus_tag="KPB88_000920"
     CDS             complement(18724..19188)
                     /locus_tag="KPB88_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0053218.1"
                     /translation="MTRISNITIAEQSEYCILAIRKTIDFMVEFSEFSKQSFEKISRY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(19624..21045)
                     /gene="nhaC"
                     /locus_tag="KPB88_000921"
     CDS             complement(19624..21045)
                     /gene="nhaC"
                     /locus_tag="KPB88_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0053219.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSFGGILE
                     KTGMLEVVASSLLRLAKGVFGIVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKVARIEDEPETCLNFDV"
     gene            complement(21075..22139)
                     /locus_tag="KPB88_000922"
     CDS             complement(21075..22139)
                     /locus_tag="KPB88_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG0053220.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIKIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKKMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(22124..23533)
                     /locus_tag="KPB88_000923"
     CDS             complement(22124..23533)
                     /locus_tag="KPB88_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG0053221.1"
                     /translation="MENSRVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQVTSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNIENQDEVRLIFEDTNYRLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDTVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILYNMFKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(23533..25737)
                     /locus_tag="KPB88_000924"
     CDS             complement(23533..25737)
                     /locus_tag="KPB88_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0053222.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEIFEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMNNK"
     gene            complement(25731..26105)
                     /locus_tag="KPB88_000925"
     CDS             complement(25731..26105)
                     /locus_tag="KPB88_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG0053223.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQTVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVNGEYWDEVTCLFREGVESC"
     gene            complement(26183..27613)
                     /locus_tag="KPB88_000926"
     CDS             complement(26183..27613)
                     /locus_tag="KPB88_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG0053224.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEVIQSQYAVGNTPIIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIRAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNSL"
     gene            complement(27606..27914)
                     /locus_tag="KPB88_000927"
     CDS             complement(27606..27914)
                     /locus_tag="KPB88_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG0053225.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIKAKEILFGGVYNE
                     "
     gene            complement(28012..29079)
                     /locus_tag="KPB88_000928"
     CDS             complement(28012..29079)
                     /locus_tag="KPB88_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG0053226.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(29346..30719)
                     /locus_tag="KPB88_000929"
     CDS             complement(29346..30719)
                     /locus_tag="KPB88_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053227.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNTKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        10071 a         5250 c         3951 g        11555 t
ORIGIN      
        1 taacaattca aaataaataa cgtatgtaag aaccgtagga actatgggaa tagcctgtgg
       61 agaattagta agacatattt tagtatgcaa aattctgtga agcaggaacc ctgtgacttt
      121 agtcatggga ggttcagaat acaactatga atgagttggc aaaggaagtt gaatatacta
      181 aacgcaccat atacaagtac ttttcatgta aagaagattt attctttgct gttgtcttaa
      241 gaggatataa aagactttgg gataatgtta aaatagaaag tgcaaaaggc aaaacagggt
      301 ttgaaaaaat aaaactttca tactttgctt ttcataaatt ttactctgta gaaccttctc
      361 tattatgcct tatgggaatg attggtattg taaactctga aaaatcggat actgatatgc
      421 tgtttaaaga aaagtttttt tcttttaata aatttatgtt tgatgaaata caagaaatgt
      481 ttgaaattgg aaaaaatgat agaagtatta ggcatgatat tgaaatacct actcttatgt
      541 attcatcaat atttacactt acaggatttt ttaatttact atctgtaaca ggaaaatcat
      601 acctgaacaa ctttaatata gatgaggaaa aatttattga aacaactctt gctcttttaa
      661 tagattcaat taaagcatag acctaagttt ataaattaaa gtttatatac ttaaatgata
      721 attttttcta tctctaatat aaaaaatatt aaacataaaa ctaataactt agttaatgaa
      781 ataactaaat tactgaaagg actaaaagta aatggctaaa atacttcttt tggaagatga
      841 tgatacaatt acatttggaa taaaaacttc ccttgaaaag aaaaatcaca ctgtatgttg
      901 ttataataca attgctgaag ctaaaaaaaa ttttgataaa actattgatt taattctgct
      961 tgatttaaat ctacctgatg gtattggata cgagttttgc caatatgtta aaagtataga
     1021 agatactcca gtgatatttt tgacagtccg tgatgatgaa aaagacatta tacgtggatt
     1081 agatatgggt gcagatgact atgtggttaa accatttctg ctttcagtac ttcaatccag
     1141 aattaatgca gttcttagaa gaactcattc taatactaac aatactatta catgtggaaa
     1201 tataagcttg aataaggttg aagtcaaagc atatttagat aatgttgaag tacctttgac
     1261 agcaggagaa tataaacttc ttctaagtct tatggaaaat aaaaatagag ctatgactcg
     1321 tacaaagcta ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg acaacactct
     1381 tactgttaca ataaagcgtc ttagagaaaa attaaaaaat ccaccttgta taaaaacatt
     1441 aagaggcata ggatatagaa tggaggacag ctagtgaaaa ctccaagaaa tatcaacagt
     1501 acagttgttt ttataatatt ctgcttaatt ataacttttt tattgagtgt aattctaaca
     1561 actataactt cttttaatga atacaatgta ctgtctaagg ctcttggtgc atcatatgag
     1621 tataatccta agtctacaga aagtatacta aaatcactga aaaattctag tgatgaggat
     1681 tttaaaacag gaaatgaaat tttatcaaaa catggatatt ctaaaagtac atcttggagt
     1741 aaaaactttt tttattttct agtggtatcg ttcggtataa tttcaacaat ggctatagtt
     1801 gtatatataa tcaaacatag aacaaataag caaaaactga gtagaataaa caatcttact
     1861 caatacttag aagaggtgaa tttaggaaaa gagtctgtac ttttacgatg tgaagatgaa
     1921 ttttctcacc ttgaagatga gatatataaa gcagttgggg aacttcgttg ttcaaaagaa
     1981 tttgctttaa aagaaagaca aactctttca gataaccttg cagatattgc tcatcaactt
     2041 aagactccaa ttacttctat gtcacttatg acacaattac tttcagaaaa ttgtccagat
     2101 gaagaaatag attatattaa caggcttaca aatcaaatat caagacttga gagactagtt
     2161 tcatctttac ttacattatc aaaattggat gcaagtactt taatatttga aagcaaactt
     2221 atagatgtac attctcttct tacttatgca attgagccta tagaagattc tttaagagaa
     2281 aaacatcaaa catttactat cgaaggttct ccttcggtag aatttgaaat cgatattaat
     2341 tggactttag aagctctttt aaatattctt aaaaattgta gtgagcatat agaagatggt
     2401 ggatatatcg ttgcttatta tagtcaaaat cctctatatg ttgagattat catagaagat
     2461 aatggcaagg ggtttgctca ggaagaactt ccatatattt ttaatagatt ttatcgtggt
     2521 aaaaatgcta gtaaggacag tattggtata gggcttgctc tatcaaagtc tattatagaa
     2581 cgtcaaaatg gtactctaca tgcagaaaat tgccataatg gtggtgcaag attcataatt
     2641 aaattttaca cataaaatgc tggattataa gtaaaatacc tataatccag ccatatctat
     2701 atcctatgtt atctattatt ttagtaatat acttctattt acatttaagt tttattaaat
     2761 aaacaactta actatcccta tacttgtaag tagtcttaaa tcgaaaccct taaataagtt
     2821 caaatagctt ttgtttatat tgtttcattt tttatagatt ctactatatt ttcattcttc
     2881 aactgcttgt atcctgtcat atatgatgct attacaataa aaagtatcaa tatagtaaaa
     2941 cctattattg tagatatagg tgcaaatggt aaatactcta taaaatatat ttcatttata
     3001 cgtagaaaag tatacactat tcctatttgc acaggaatac taagtagtaa tggtgtaatt
     3061 cctaagaaca aaccttccaa aacaagcatc ttttttatgc catctggaga taatccaaca
     3121 gaacgcaaca tagcaaactc ttgtcgtcta cttctaatat ttcctgacac tgtagcaagt
     3181 acatttgata atccaatcat agccaataaa cctgtaataa aacctactat caaattcatg
     3241 gtctttcttc cactagcatc ttgtttttca ctttctaaaa catcctcaat actatagtct
     3301 cccgaaccat agtctttatt acaaattgac tctaattttt ctcttactct aggaatgtcc
     3361 tcacgattat ttactttaaa tagtcctata agcctatttg aacgtaacct acctacttca
     3421 tcataatttt tagatatttc ctgtaccata gacattggca ctatttgcat taatgtatac
     3481 cttgaactag tatctattat tttaggaagt gtgcttgcaa tagctcccac tttcatgttg
     3541 taagtataat cccctttatc ttcgtcatat actttttcat ttaatttaat gttatctcct
     3601 attgaaagat ttaaataatc tatatatact ttatttctac gtgtgctacg attaacatct
     3661 tcctccttat tatatacaat tgaggttgga ttttttgtat cataaaataa ttttgggtct
     3721 acagaaattt gcctacaata ttctgtaaaa ctcttatcat caagcccaat tatatttgaa
     3781 attatacgaa acttgccatc acgttctata ggagagtatt ttttagatga aactatactt
     3841 ttaaatcctc catttttcgc aagttctttt gaagcatctt tttcagtcag ccaagtggca
     3901 gcagacattt tttgataata tagtgaactt tttacacctt ctgtattatt taatttttca
     3961 ataaaagcag actctacagg ttcactattt tccaaataaa cttctatatc tctttggtcc
     4021 ttataattat cacttccata aacagctttt gctccttctt gattggcaat tatatgcaaa
     4081 aaacctgtca gtaataaaaa agataatgtt aaagatattg ttgcagtacg ataagatttt
     4141 ttacgagcac gtagtgcatt tgatgcaagt tctccttcta ttccaaatat ctttcctaat
     4201 gaatatttat gcgacttttt gattttaaca ctatctcctt gttgtattgc ctctatagga
     4261 gatatttttg atacttttcg cgctggtata agtgcagaaa tccagactgt tactattgta
     4321 agtaaaactg ctggcagaaa agcaggaata ccataagtaa ataccacttc tggagcatca
     4381 gtatgataat taactgaatt aatatagact ataagcctat tacacaataa ccatcctaaa
     4441 aataaaccta ttggtaatgg tataattgtc agtaaaaagc cttcaaacac tacagacctt
     4501 ttaatttgtt ttggtaatgc accaatacta gataatatcc ctaattgaat aagtctagcg
     4561 tttgcagaca gtgaaaatgc gttgtaaatc accattacaa acacggctac tgtaaaaatt
     4621 gctattgtaa gatacataat aggagttgaa aattttgata tactactcat agatgctttc
     4681 tgttctgggg aaaaaactaa catctttaaa agataattac tattgtattt aagattgtat
     4741 tttccatatt cattagtctt ccaccccaat gatttagcaa gttttggaag ttccttatac
     4801 gtgtctctta tatttttaaa tcgcaagtac acagttatac tatcattagg ttttatactt
     4861 gttttatcaa gatagccaag agctgtatat gcaggtatta ctgatgaagt agaaacatct
     4921 acctttccta ctattgtcaa tgtagtctct gaaatttttt taaaactttc ccctttcgat
     4981 tttggggcta tgggttcaat aggctttcca tttaggattc tatctcctat tgggagagta
     5041 attttatcac caatttttac acttggatta ttctcaaaat attgttttga tagtgcaatt
     5101 tcattagcat tatttggtcg acgaccctct attatagtgt ccttttctgg catatgattc
     5161 caatactctg atgtagcatt tctataaatt atataatctc ttttgtcatt attaattttg
     5221 cctacatacc attgtccttt taccattaca tcatcaactg aagaaaaatt ttcaattaac
     5281 gataagtctt ttccataagt atcgtcaaac aattcaccat gccagttacc ttctttctca
     5341 attgaaagtc ttataaaatc tgtccacatg gtatacaaca gtccacacaa ggaagacatc
     5401 atggttgtag taagtaatat agctaacatt atagctatac tactacgttt attgcgtcgt
     5461 acaaagtcta aggtgtattc ttgaagaata ttcatttctt caccaccttt tcagagacaa
     5521 tcactccatc ttccatagtt acaatacgat ttgcctctag tgctattttc tcatcatgag
     5581 taataagaag tattgtctga ttaaatcgtt tattcgataa gtttaacaaa tctacaattt
     5641 cttctgaatt ttttctatcg agattaccag ttggctcatc tgctaataaa atagctggtc
     5701 tatagattaa acttctagct atagataccc tttgttgttg tccacctgaa agttgacttg
     5761 gaagatggtt taatctatca gataatccca atatagatac aatctcagag aatctaccct
     5821 catccacctt tcttttatca agtaacatgg gtaataatat attttttcta acatcaagag
     5881 taggtataag attataaaat tgataaataa gacctacttt tctacgtcta aaaactgcaa
     5941 gagcttcttc ctttaaactt gatatttctg tatcttcaac ataaatcttt ccagatgtag
     6001 gtctatctac tcccccaagc atatgtagca atgtagattt accagaacca gatgctccta
     6061 caatggatac aaaatcacct ttttgaactg ataaatttac attattaagg gctgtcactt
     6121 tggtttgatt tgaaccatat atttttgtga gattttcaca ttttaatatt tccatataca
     6181 atcatctcct ttatggttaa attttagcaa cctaaaatga cattatagtg acataaatat
     6241 aaatagcgat tactattaaa agtattgttt agtttttgca cattatttaa aattataata
     6301 taaaacaaaa actatgcaat atattttatt tatactttta atatattaca tagtttttaa
     6361 aagatattaa ctttaaatct ataggtattc aatttaataa tcaatagcaa gtattttatg
     6421 tactaaagga gttttgctct atatagactc aatttagtaa aattatttaa taaatattac
     6481 agactatagg taaccaatat ttacaaataa aattaagttt attcccttac actataatat
     6541 ttcttaatta tattaattgc aatttccttt acctttggcc ctgtaatttc tttattggaa
     6601 tcatctgatt taatagcaaa ataataggtg tcaccttcac gctctagcat accaacaaac
     6661 catgcatcca cacgtttatt tttttcatca aatcctgttc cagtttttcc atatacattt
     6721 atgtttttat catttacatc tattttcata atatctctta aaatattaat atgctctttt
     6781 ttaaagtttg tatctccctc aaatattttt gccattgttt gcacttgttc ttttggagat
     6841 atttgtaatg acgattctaa ccaaaatcca tttaaatgtc cctttccatt ttttaaatct
     6901 ccttcccatt cactaatatc gcaattccca tactttaaat catctaaaat gttttggaca
     6961 gatttagcat caactttatt tatcaatttt ttgtagtacc aaacacaaga ctccttaaat
     7021 gcctcttcta aactaagatt tttattccaa tttttatttg gataatctgt accatcatac
     7081 cccataactg attcttttga gtttattacc cctttttcta aaccaattag agttgaaact
     7141 attttaaaag tagaacatgg tgaccttctt gtttcaatta attctttgtt atatatatta
     7201 tattctttat ttttagtatt acaaaaaata gctcctccac taataccttc aaaacaatca
     7261 ctatagtcaa ctatattaac gctttctttt ttattcttat cattattttt aattttagat
     7321 ttatttaatt cagattttat ataattctta tcagtttttt gatttatatc gttatgtttt
     7381 ttcactgcat aatacattac cataactaca ccaaccaaca agatagctat ccaaataaaa
     7441 ttcttttttc ttttcataat ttatatcatc tcccatattt aaatttataa cctactctta
     7501 aaatagtaga cattatttgt aatatacacc aatctaggtt ctacttataa gaaacaagac
     7561 taaatgaatt gctgtatccc ttatattttc ttcacctagt acaattatat tgactaaacc
     7621 tattacgaag ataattaggt aactattaat agtattctat cctcctttta atttgtgata
     7681 taataattga ttccacattt aaaacttact attgtaatat ttaaatatta caatagtaag
     7741 ttttaaatgt caagctagtt ttggaaagtt attttaattg tataattgtt attacattta
     7801 taaaatatgg catgtttaaa attattttgc tattgatagg attaagataa tcaatattat
     7861 aaaaactata agttataaaa tttaatatat aatttatagc ttttactttt acaaatattg
     7921 tttttattga tttattaatg aattttattg acaggttata tttatataag taaacactta
     7981 cataataagt atataatcta catatctata agttgagtca attcaccagt atatgccaac
     8041 ttaagtttat gcatagctcg tgtacaagca atatacaaca aactgcggtc ataatcacta
     8101 taatatgttt tattatttac tgaaggtaca ataacttcat caaactctaa accttttgac
     8161 attttaatag aagttatgga tactcctttt gtaaagctag aactttcaga agatattaag
     8221 tgaacactat attcttctct taacgcatca tatacagctt tagcgtcatt atctgtcttt
     8281 aatataatac caagagtagc attatcactc tttttaaatt cttcaatttc tgtttttatc
     8341 ttatttattt catcttgttc gttattacat tttaataaag ctggttcttc gccatgtcgt
     8401 tcaataggct ctaatgaaga tatgtcttga acctttttag cgaaattaat aatctcaaaa
     8461 gtagaacggt aactcttgtt caacataact agttccccat cattatatag ctgtttcata
     8521 tcatctagtg tgtgagcatg attgggatta actaattgtc caaaatctcc aagaatagtc
     8581 tttttacact taaaaagtaa gtttattaca gcatattgaa taggtgtata atcctgcatt
     8641 tcatcaataa caacatgtct tataatctta tcttcttgta taccttcata tgcagcatga
     8701 atatatataa atgggtatac atctgaccat tcaagtgttt tctttgctgc catcacaaac
     8761 atgtttgata tattcatttg tttaaagaaa tccttatata gtgtaagagt attttttatt
     8821 gtcaacatta cattcaaact tttaagtata ctttttacct ttggtaagtc ttcttccata
     8881 atattatctg attcaaattt ataatgaatg tcttcagcta ccttctctaa tctcttctta
     8941 actggatatc tattatatgc ctcaaatcta ctctgtatcc agtcgctttt agctgtaaaa
     9001 cttccaaaag tgtaatcttt tgggataaaa atcttatttg gcatttgttt tatataatca
     9061 tcaataagtt ttacaaaatc aagtgtagat ttaaaacgca ctctttcagc ccatttagca
     9121 tcatttattt ctaatgggtc tttctcactt tcaaaattaa taactctatc taattgtacc
     9181 tctgctatat tttcaaaact taattcacat aatggctctt ctccaagctc aggaaggaca
     9241 ttggaaatat agtctccaaa gactttatta ggtgatagga taattacatt gtttgctgat
     9301 attttatctt taaatcgata aagaagaaaa gcaattctgt gtaatactat agatgttttc
     9361 ccagaaccag ctaccccttg aataatcagt gtatcagctt tatcatttct aataatttta
     9421 ttttgttcct tttgtattgt tgaaataatt ggcttcattt tttcatctga agtgtgacta
     9481 agctcctttt gaagaatatc atcttgtata ttaatggaac tttctagtgc atattccaat
     9541 tttccatttt taattttaaa ttgtcgtttt cttgttattt ctccatccac ccatcctatt
     9601 ggagcatcat atcccgcttt tccgatttca taatcataaa acatactggc aattggagaa
     9661 cgccagtcaa gtattataag ctcatcttca taatcaaatg caaatctacc aatataatac
     9721 ttagattcat catccatatc tttaagccta aaatctattc tagcaaagta tggagaatct
     9781 ttgattttat taatcttatc tcgaactttt accataaaat ccccatagtt atctatttgt
     9841 tttagtcctt gttcattttg aaacatttca tgtgggtcta tttcaccatg attttttacc
     9901 atatacattt tagcgtccat ataatcctta tcaagcttgt caacaagatt ttcagctttc
     9961 ttaaaagcat tgtctagctt gttatttatc tctattagat gtgcaatctc atctggaaaa
    10021 aaagttaata actctgtatc atgttcacac atattatgtt tagaattgga tgcaataata
    10081 tcgcaattta ctacttgttt attttcagac atttaaatcc tcctttgtta attaagtact
    10141 tacactgaat cattatactt aattgtgttt tggagttttt aatcattctt gctatttaac
    10201 ttaaatgtta gctttaaagt tcctttttta aaaaactaaa tctttctata tttattacca
    10261 taagtacact tacaagcaaa atacattaaa agcagaaata ctcttatata taaagtaatt
    10321 caaacaaaaa atatgtggta aacttttcat ttaatctaca attaattttg tctattatct
    10381 ctttcttcta cataaggaag aataaatttt tgagtaggat atccaaattc cacaagcaat
    10441 tctgcttctt caaaccccaa cttctttatc atatctctat gtcctgtatc agctttgtca
    10501 ccctctcgaa aggtagtaat tgagatatct gtatctatca aaaaatcttc aataactttc
    10561 ttcaaaaaca cttgtgcaat gtctgaatta cgatactgtg gatgtattcc aaaaaaatca
    10621 atacttctag tttctctatt aaacaccata tttgcaatag caacattatt aagttttaat
    10681 attaaagctc tttcttctaa aatatactgt tctaattgat atatatattc ttcctcttgc
    10741 aaatttggaa atccatctat gactaatctc actaaattca tccataatgg tatgtcagac
    10801 atatttgcaa gtttaatttc tttttccaac tctagtacat tctttgaaaa taggttatct
    10861 tttttcaaaa caaatcttag ctgcaaagga taaaattctt gttctttcct atatttattt
    10921 ggagattttt tatacatagc tttaaatata gaagtaaatg cctgttgact ttcatatcca
    10981 gctatcaatg caatttcaat aattggtttc tttgaaaata ccaataattt tgctgcttct
    11041 gtcaactttc tccgctttat gtaatcatgc attgttagac caacagaagt tgtgaaagtt
    11101 ctatgtaggt gatattttga gtaatgaaca gcattagcaa cgacatctaa atccagcttt
    11161 tctgataaat gctcctctat atagtcaata gcagttatga cattaattac tttgtaagac
    11221 attgcagctt ccctcctttt atccatgcaa cattatatca attattactt agttatattt
    11281 aatgaatctt gctattttat aaacaaattt attgtttaag ttttcaattc ataaatttaa
    11341 tatattacct tttcatattt aaatttcaat ggttatagtc actaagtaat tcgtaaaatt
    11401 taactcaagt atgattgtgc tgtataggat attactcatc gtatttttct ggaatttgaa
    11461 tacaagaagc tgaataaaaa ctatactttt ttttcatata ctctttagca tgttttaaag
    11521 cataccgata gctatctgta atatcattcg caaaagattt catgtctcct tcatgcagat
    11581 atttaactgc tacaatgatt tgtttattga gatttttaag tactcgtttt ttattatcat
    11641 aataagatat atagtgtccc cattcagtaa gatgaaatgt ttcagttaat ataacacgca
    11701 aaggttctaa ttctatatgt tttaaaatag cctcaaaaat atcaattata caaatagaat
    11761 taggcagaat gagtttttca attaactcat ccaattcact tgaagtaaac tttggagctg
    11821 ccattaacgc tgctggatga ataattaaca ctaataattg gagggcatgt aaatattgca
    11881 gtgcttcctc tgtacgtctt gaattagaaa gcaattgctt gacttgagaa tcatctggtt
    11941 ctataacgac agttccttta ccatttagag tctttataag tcctctttgt tcaagttcag
    12001 ctaatgcttt tctgattgta gatacagaaa caccatattg gttggccaat tgtttttcat
    12061 aaggaagata tgttccacga ggatattctc cattgccaat tttttggttc aaatcattga
    12121 ttattcttga ataaaagtaa tctttaccac gagttggttt ccaagaaaaa gatatatcat
    12181 attgagtttg atattgaggt gtagtatttg ataacttttc taaaacatca gcaattgaat
    12241 ctgaaaatct ttgaaataaa tttactaact gaatgtactt aatagaaagg tcatttcctc
    12301 ttaagatatc tacaataact cttgtaaaag atacaggctt ctgtagaaat ttttctaaaa
    12361 agtaaggaca ctcttctata aaaaaactta agttagaata aagttcaaag gttgaataga
    12421 gattactgag taaatgatta cctccaattt ctaatatttc tttaataaaa atagaagtag
    12481 aacgccatcc acctgcagaa atcccaagac gtgatatttt catagtcttt ttatagtgat
    12541 gtagcatttc caagttacaa ttctgtgaag caaaaaccaa aagtggtggt agtaataaag
    12601 catatgtttg atatatctgt acgatatcat cccttttcct caaaatgtca gataatatat
    12661 cttttgattc aggcataact gttcttgaaa gaacgattgg ggcaatacgt ggcttaattt
    12721 caatcaagcc ttcttctttt aatgcatcaa aaacacgatt aattgtatat cgactaacat
    12781 taaattgatt gcaaagcatc cttgaagatg gcagataatc tccaggaggt atatgattat
    12841 tcatgatacg ttgcttaatt tcactataca caaatgagaa cttggtttct tgtaattcca
    12901 taattaactt cctctcaatt ctatttactt tataaatata ttatgtatat tttattattt
    12961 tgtcaacaag gaagtacaaa cctatttgta taccttacca ataaaaaaca aaaggtatct
    13021 gtacacctta ccaatatgat tattgttaag taacataagc aaaattataa taaaaatgac
    13081 tataaaaatt attatgaaag tgactgatgt attgtgaaaa aacaaaaaat tttaattgta
    13141 gacgattcag aaatgaatag atctcttcta atagatattc ttgagaatca atatgacatc
    13201 gtagaagcta ataatggagc agaagcaatt acaattcttt cacaacatag aacagatttc
    13261 tcactcctac tattggatat tatgatgcct gaaatggatg gatttgaggt tttagcttat
    13321 ataaaaaagc atcattggga tgacacattt gcaattatca tgatttctgc tgataattcc
    13381 actactaata taaaacatgc ctatgatttg ggagcatttg actatatcgg tcgtcctttt
    13441 gattcagcaa ttgttcatcg tcgtatttca aatacaatgt tgttgtatgc taggcaacat
    13501 tatcttgaga atattattac aaaacaattt catgaacagg aaaagaatag taagttaatg
    13561 atttcaattt tgtctcatat tgttgagttt cgtaatggag aaagtagttt acatatacaa
    13621 aaggttaata tcatcacgaa tcttttgtta agaactttgg ttcagcaaac agaacaatat
    13681 tttttatcag agtcagatat tactttaatc agtacagcct catcacttca tgatattgga
    13741 aaaatctcaa tacccaataa aatcttgaat aagcctgggc gactcacagt tgaggaattt
    13801 gatgtgataa aaaaacattc aaaaattggt gccaaaatgt tacaggaatt atctgttgag
    13861 caacaagagt catcgcttgt taaagtagct tatgaaatct gtcgatggca tcatgaaaga
    13921 tatgatggaa atggttatcc agatggatta aagggagagg aaatacctat atcggcacaa
    13981 gtagttgctt tagctgatgt atatgatgct ttaaccagtg aaaggtgtta caaaaaagct
    14041 tattcgcatg ataaggcagt ggagatgatt ttaaatggca aatgtggatc atttaatcct
    14101 atccttttgc agtgtttaat aaatatttca gatatacttg aaactaaatt aaaggatgta
    14161 gcgaatgtaa aaccaaatac catagatatt aggaggatgg ttgaaaagag aaaaaatatg
    14221 atacatgaaa ggtgtacaaa ctatttaaca tacgaaatat tatatactga ccccttaact
    14281 aatgtgtata accgtagata ttatgaagac catattttaa atttaaatga gattcaaaat
    14341 atcacattaa tagatgttga taattttaaa gctattaatg attcctatgg tcattatgta
    14401 ggagatattg ttttacaaga aatatccaaa acaatatctt catgtgtacg aaaaatagat
    14461 actgtaattc gctatggtgg tgatgaattt atcattatat tttcctatat accaacaaat
    14521 ttatttaaga agagattaga gataatacga acttctgtta atatgataaa atttgaagaa
    14581 tatccagaga tttgtatatc tgtaagtata ggaggaatat gtgaaacaaa taaagcgaca
    14641 catctcttta aaattgcaga caaactcatg tatcaggcaa aaacaaaaag aaacaaagta
    14701 atttacgtta ctgacagaaa tactgtagat attaacgaaa gagaggaagt ataaaatgaa
    14761 tttaagtgat tgttatataa tatttggtgg taattatgat gaagtacttt gtagactaca
    14821 gcatgaacac atagtgcaaa aatttatttt caaattttta gatgacaaaa gttttaactt
    14881 actaaaggtt tctatagata atgaaaatta tgatgatgca ctccgtttcg ctcatacact
    14941 aaagggaatt tgccagaatc tttctttttc taggttatat gaaagcagta atcaaataac
    15001 aatagagtta aaggatggta attataaaaa agcagttgat atgataccac aattatcaaa
    15061 ggactattat aaaacaattg atattatcaa agaatataaa atgtctaagg aaaaataaca
    15121 tccatgagca gtggcaaaac aaaaaatcat ataacacgtt tcctagctta cagcttcatt
    15181 ggactcatac tctttagtgt agctattttt agtatattag gaatttttat gaactcaaga
    15241 agtcaaaaaa gcttttacaa agttggagaa atctacatgt ctggaatgaa tgaagaaatg
    15301 tccaaaaact ttgaaaatgt tatcaaacta cgttttaacc aagtagaagg cattatttct
    15361 actgttccag aagatagtag taatagagag aaactttatg aagaactcgt ttatagagca
    15421 caaatcagag attttgacta tttagcagtt tgctctgaag atgggcattt tgaaacactc
    15481 tatggagaag caatacagcc aaaaaatcca ttgccctttg ttagagcatt aaagcaaaaa
    15541 aatcgacgag ttgctgtagg tgttgatgct attggaaacg aagttgtctt gtttggtatt
    15601 gatgctaatt atcctatgca aaatggtgag gaaggcatag gtctggtggc agctgtccct
    15661 ttaaattata taaccgaatt tctttcttta aaagataaag gagatatgat gtattatcat
    15721 attatccgtc cagatggcag ttttgtaata cgaaatctta acactgaatt gtgggagtat
    15781 tttgacctat tgcaaaagca acagaatcaa gtaacagaag aatcatcatt aaaaaatcct
    15841 cttgtgggtc ttgatgtagc attgcaagat aaaaaaaatt atagtacagt ggttgaagtg
    15901 aatggagaat atcatcaaat tcatggtaca ccattgtctt attctgaatg gtatttaatt
    15961 gctgtaatgc cttatgaaat gttaggtgaa actatgaaca gtctaagtgt agaccgtatg
    16021 gttgctacaa tgctggcatg tgcttctgta ttagtttttc taatagtaat tttcctgaga
    16081 ttctttgtca tgactcgtct gcaattacag gaactcgaca agaatcgtga gatggcactt
    16141 gaagcaaata aagctaaaag tgaatttcta gctaatatga gccatgatat tcgtactcca
    16201 atgaatgcca ttgtaggtat gactgcaatt gctaaagcac atatagatga ccaagaacaa
    16261 gtaaaaaatt gtctaagaaa gatcacactg tcaagtaaac atctattagg acttatcaat
    16321 gatgttttgg atatgtctaa aattgaaagt ggcaaattgg ctttaacaat ggagcaaatt
    16381 tctttaagag aaattattga gggaattgtc agcattatac agcctcaagt taaggtaaaa
    16441 aaacaaaatt ttgatataca tattaataat attttaacag aaaatgtgtg gtgtgatggt
    16501 gtacgtttga accaggtttt actgaacctt ttatctaatg caactaagta tacacctgaa
    16561 ggaggctcca tacaattaga tttatttgag gaaaaatctc caaaaggtga agactttgtc
    16621 cgtgttcata tcaaagttaa ggataatgga attggcatgt ctccaacatt tttgaaaaaa
    16681 atatatgaat cctatagtcg tgctgatgga accagaatac ataaaacaga gggtgctggt
    16741 ttgggcatgg caatcacaaa gtatattgta gatgcaatgg aaggaactat taatgtgcag
    16801 agtgaacttg ataaaggtac agaatttcat attatagttg attttgaaaa agctagtatg
    16861 atggaaatag atatggttct gccctcttgg aatatgttga tagttgatga tgataaatta
    16921 ttttgtgaaa cagctataaa tacattaaag tcaattggaa ttaaagccga atggacatta
    16981 agtggagaaa atgcaataaa attgataatt caacatcata aaaaaagaga tgattatcaa
    17041 ataattttat tagactggaa acttccaggt atgaatggta ttcaaattgc caaagaaatt
    17101 cgacgtaatt taggaaatga aatacctatt cttctaattt ctgcatatga ttggagtgaa
    17161 tttgaagcag aggcccgaga ggcaggtatt agtggtttta tttctaaacc actgtttaaa
    17221 tcaactttat tttatggatt gcgtccatat atggacattg gaacaacaca agaccagatg
    17281 ttgaacaata atattgatat gtctggattt cgtatcctac tcgctgagga taatgaactt
    17341 aattgggaaa ttgcaaggga aatattgtcc gacttaggtg tggaactgga ttgggcagaa
    17401 gatggccaaa tatgtctgac gaaattccaa gaatcatcca aaggatatta tgatgttatt
    17461 cttatggata ttcgaatgcc tcatatgacc ggatatgagg ctacaaaagc aattcgaaca
    17521 ttagatcatc ctgatgcttc ttctattcca attattgcca tgacagcaga tgctttttca
    17581 gaggatgtca aacattgttt gggatgtggt atgaatgctc atatagcaaa gcctattgat
    17641 gtaacagaat tgacacgttt attgaaaaaa tatttaatgt aagtttccta aaacctagat
    17701 tatgacaggc taattgacaa tagctcagat attagagttt actctaatat ctctttgtag
    17761 tggctttcta tatattctat acattttcta taaagactca ttgctcctat tctatcatca
    17821 tttgattgat aggagcaatt tttacaacat aatatgtggt tcttctatct ctattagatt
    17881 tctctatatg tccataccta agacattttt gagctgtata cttgggatta actattatta
    17941 ctattgaacc atacatttaa gctttatatt ctaatagttc tttaaattgg taaaattcac
    18001 acgatacaaa aacatatctt ttattaatat ttactttctc taaggaactt ctaacattag
    18061 ttaaatcttc aagtacaact aatgtatttg caccatattt attaactagt gtcttaataa
    18121 ttaattttta actaatcctg ctttgtatgt tgaacatatt ctataattgt accatctgga
    18181 tgtctcaatg tcatattata tcctgtagga acttttttct tatctcttac tacaactgct
    18241 ccattatcca atagataact tctccattca tcaacagaat caaccataaa agtagatttt
    18301 gtttctataa atggtttaag agcatcatca gaaccagata atagtaaaac atttcctatt
    18361 tgtgctaatt ccaacccaac ttctttatag gaaaaacgca aagaacattt ttcttttaaa
    18421 agattctcat aaaaaggtat ggctttttct atatcttgaa tataaaaacg tgttaatata
    18481 tcgtatattt tcatattccc cccctatata tctatatata gtattacttc taaattatac
    18541 cactctacaa attatataac aataaattaa gcatcacctt ttgagcatcc taaatacacc
    18601 ttaacaatat atttgggtta cattagaata cataaattaa atctctttga tattatttag
    18661 gataaaattt atgcagtaaa tgtccttcta attatcaact aacaaacgat aatctttaac
    18721 tttctatata atgggtaaca tcattttcgt aagatattca ctagctgggc gattgatatc
    18781 atttaaatac tgataatata tatcgccatg aagtttatat ccattgctat ttgcccatga
    18841 aaataagtct tctaaagtag ggtcttgtaa ttcataaggt cctaaatcaa ttgcagttat
    18901 tattttctgt gcaggaacaa tcctttgtag aatctcattt ttcccatcta tataagtggc
    18961 tacaggaaat cctacttcaa catccaattt tgctaaatcc atgttatgaa aacatacaat
    19021 tggtgctcct gaagataaaa ttcctctctc ctccagatat ctagaaattt tttcaaaact
    19081 ttgttttgaa aactccgaaa actctaccat aaagtcaatt gtttttcgta ttgccaaaat
    19141 acagtactca gattgttcag ctattgttat attagatatt ctagtcattt tattcctcca
    19201 ttactttatt ttcatttcta atctttttat accaaatttt acgaaaattc actgtgtaac
    19261 taattatttc gtatgaatat aaaacataac ctccttccat ttcttatatc gaaagtatag
    19321 caaaaacaat ctcatcattc ttgtcacaca gtgctcttat aattagaaca tacttaaaag
    19381 acttgcctaa tttttaggca agtctaaaag aaatcttttt tctataaaaa atttatacaa
    19441 acaattcaaa tatcaagata taaactaata taaaactata aatttaaatt tactaatctg
    19501 aacctcccat ggctaaagtc acaggattct tgattcatag aatttcgcat actaaaatat
    19561 gtcttactaa ttccccacag gctatccccg tagttcctac gttcttacat aagttatcta
    19621 ttatcaaaca tcaaaattta aacaagtttc aggttcatct tctattcttg ctactttaaa
    19681 cttacatgca attaatacta aagatactat tggatttata atatttaata aagcgtatgg
    19741 accatatgtt agtgcagata cacctaatgt tgctgttata tacgccccgc atgtattcca
    19801 tggtactaat ggagaagtta aagttccact atcttctaaa gctctcgaca acatcttagg
    19861 atgtatacct ctctttttat attccttatt atacattctt cctggtataa ctatagatag
    19921 atattgttca cctgctatta tattggtaat tgtacaagtt accatagtac ataatactat
    19981 tccaaataca ccctttgcta atcttagtaa agaagatgct actacttcaa gcatacctgt
    20041 tttttctaaa atacctccaa aagataaagc gcatattgtt aaggatacag tggacatcat
    20101 attcataatc ccacctttag ataataaagc atctacctcc tgtatttgtg ttgacgaaac
    20161 atatccattt tgtgctgcat ctaatatcgt attcatagat tctccttgaa aaactactgc
    20221 aaacaatact cccaatacag cacctactat aagccctggt atagcaggaa ctttaaatac
    20281 aactaatcca attactattg ctggagctaa taataatact ggtgataaag tattaaatgt
    20341 agaatctaat gtagacctaa ttagttctat ttgttttata tctagtgctt gacctgaata
    20401 cttcattcca attacaccat aaattactaa acaaattaat aatgatggta tagtcgaata
    20461 tagcatatat tttatatgtg taaacaaatc tgttcctgcc attgctggag ctaagttagt
    20521 cgtttcagat aaaggtgata gtttatctcc aaagtaagct ccagatatta tagaacctac
    20581 gattaaccca attggcatac ctaaactttg accaattcct aaaagagcta ctcctactgt
    20641 acccattgta ctccaagatg aacctgtcgc aagagatact attgcacaga ctaatgttga
    20701 tgctactaaa aaaatatttg gtgataaaat ttttagtccc cagtaaatca tacatggaac
    20761 tactccactt actatccaag ttcctataag tacgcctatt ataattatga ttaagattgc
    20821 ttgcattgcc atctttatac tattaaacat acttaattct aactctttcc atttaaatcc
    20881 aagtctatat acagccacaa gtcctgctac tgctgcagct cctattagtg gtacatgagg
    20941 tgatgaacca tacactaata ctcctagact taaaaataat atcaaacaag taattggtat
    21001 cattgcatca aacagacttg cttttctaca ttctcgctct ttcataaaat agtttcctcc
    21061 taattattta aacactacac tataattttt tttgaaacat atttgctcgt actagatgac
    21121 atgatacccc cataagttaa taaaaattct aaattgtcac ctaatttata atcatatttt
    21181 gtgttactta catctagtat catgtggtct gaacttgccc ctaatatttt tatatcagtg
    21241 tctattggaa ttaaagagtc aatatttata tcttgtttac ctatagctat gattgccctt
    21301 tttaagattc ccttatcttc ataaacaggt ttatttctaa aggcatcaac tcctatttct
    21361 ccaataggaa ctgatggctt ttctttacat tcaactactt ggcatattaa tttaaatgca
    21421 tcttgatatg ttccatttat attttcaccg tatgccgttt ctctaccaag tagaatagat
    21481 tcgcctattc tcaaattagt aataccttct ggcatattat tatttatcaa cagatgaata
    21541 gaacttgaat tacctccaga tactatttct aggtttattc caaatttttc ttccattttt
    21601 tttgctatac ttactagtct gcctatattt tccctagatg gtataatagc tccataacag
    21661 gttaagtttg tagctatacc aattatttta atatttttta atcttactat ctctctcaca
    21721 ttattaaaaa agtcttcttc ataaaaatat ccttctcgta aatcgcctaa gtcaaacata
    21781 agaactatct tatgaataat atttttactt tcacataatt catttaattt ctttattgtt
    21841 cctaattctg agttaaaact aatatcacaa taattgataa cttcttctaa ttcacttaac
    21901 attgggattc taatcaatat cttaggtaga tttatatctt gtaatttttt taagttttgt
    21961 attcttgaat ctgctatatg gtctattcct tcactacata tttcttctac tatttctttt
    22021 tgtgcacaaa atgatttagt aacaaaagat atcttaatat ttctttcatg acacatctgc
    22081 gaaattaatt ttgcattatg ctttaacttt tctatatcta tttctaacct aggatacata
    22141 atctttcctc ctcaaaattc aaacttttta acaggagctt aagagatgct tcttcatatt
    22201 ttttagataa aacacccata gatgccataa tataatcatt atctattaat atctttgttt
    22261 tttcctttgg aaatagtaat tcattatttt tattgtattt gaacatattg tataatattt
    22321 caatcctact agggagccta gtaagtgctc cccctgtacc aactatatat cttacttctg
    22381 ttaaatcttt cccttctgca attttgactt ttccagttgt tccatagata tttcttattt
    22441 ttcctgaatg tcttagcata gctttaacaa cagcttcttt tgtcaatctc tcaacaaata
    22501 atatttcttc ttttgatttt ggtattggag gataattctc tattactgta tcaatatcta
    22561 tagataattc actttgtaag ttttctttac ctatcacttc tactatattc ttcatattta
    22621 catatacacc taaatcacct tcgacacttc tctttgcagt aggttcagga ttaactaaaa
    22681 ttttattaat atagtcactt ccatctgtaa cagaatgtac atcagtagtt gctccaccta
    22741 catccagagt taataaatct ccaatatttt tatataaaag tttagaagct tccattacag
    22801 ctcctggggt tggagtaata ttttcattaa ccatttcttt aatgtatttc attcctggtg
    22861 cggtagttat gtgttcttca aatacacttt gaatgattct tcttgtaggt tctatattca
    22921 ataaatctat tttagggtat acattctcag ttatataaag cctataatta gtatcttcaa
    22981 atattaacct tacttcatct tgattttcaa tattaccagc atatataact ggtataccta
    23041 gattcataga agctatcatt tctgcattat aaatagctgt atctctttct ccataatcga
    23101 ctccaccagc tattaaaatt atgtttgggt ttatttcttt tattttattt aaatcagttc
    23161 ttttaattct acctgatgtt acttgacgaa taacagctcc agcccctaaa gctgcttctt
    23221 tagctgcttt tactgtcata tcatagacta atccatgaac agtcattttt aatccaccag
    23281 ctgcactaga agttgcaaac atatcattat actctatatc atctacagat aatttattag
    23341 ctaaatcttt aatagcacct gataagccat ttcttacatc gccaccttca aacactgttg
    23401 ttggagcttg accttgaccc aaaaatatag gattgttagt attaatatcg tggaaagcgt
    23461 ttaccaccgt agtagtactt ccaatttcag ctaccaacac atctattttt aaagaaactc
    23521 tgctattttc cattatttat tattcatttc tcttcttttt tcaactaaga aagtggctac
    23581 atgtatgcct ttgctgtttc ttccaaatcc agcatctatt ccttgttcaa cggctatttc
    23641 aggtgttact tgtgttcctc cacacgcaat cattacttta tctcttatac ctttttctac
    23701 acaataatcg tgtaatttct tcatgttttt atagtgaata tcatcatgac ttattattgt
    23761 agatgcaagt attgcatctg cattcagttc aatcgctgca tctaccaatt tttctacagg
    23821 tacagaagtc cctagatagt ggcattctat accatatttt tcaatacctc catgttttat
    23881 atctattatt tctcttaaac caactgaatg ttcatcttct ccaaccgtag ctgaaactat
    23941 tttcataggt ttttcttcta tatctgctct tatttcttca tcagttagta ccttaggttc
    24001 ttctggtatt actaaattgt ttaaatctat atcaaacgga actcttcctt tcacttctat
    24061 cctagtccct tcagattcat gcataacttc tctacttata acctcaattt cttgtaagtt
    24121 catttttttg acaaattcca aagctgcaaa ctctgctgtt cttttatcag taggaagcat
    24181 cattgttatc attacaattc catctgcaca ccattccatt tcaggcttga ttttagttcc
    24241 atttctgtac tcatatgatt cttttagtct attttccaca ttatcaaaat cgtcaagctc
    24301 atcaatatat attattttgt ctggattttc aaatgtacat ccatctatta acattgatgg
    24361 attatctata gcattttcat cgtattgagc tacattattg tatccaaagt gagcagttac
    24421 aggtgctaaa taatcttctt ctctttcata aacagtacct tcaccaactc caccttttat
    24481 ttttctagat attccatcac catttctttc tggataattt ccagaatcaa caaaaaatcc
    24541 ttgttccaca gattcaaaat atccaccaac ttcaaatatt tcttccatat ataatacagc
    24601 tctttcttta agttctcttg ctttgtctct taattcaccg acatctttaa gttctatcat
    24661 atccataagc ccatccatac caactaaagc ttgctttgct gtatcacatg cttctatatt
    24721 gtatatatgc caaggtacat ttctaccttc atcaggtgtt atagttgatt gtatgtcagc
    24781 tctagttaat actgatgtta ataagtttaa tacatgagta actgttgctt ctctagttga
    24841 tgattccata tatttagtat tcatttgagc tctcatctta taatcactaa ataactctct
    24901 aagtgcaaca gcataaggta agtctattct taaacatggt gctggaggtg ctgttggtgg
    24961 aactgttgat aagcatatat tacttttatc tatacctatt ttttctgaga atatagagtt
    25021 taaagcgtgt tgaaccataa gttcaggcat taccttccat gcttctctag cagtagcatt
    25081 agcattatgt gctccgtcaa tttgagctat attggcaaaa gccattattt tctttgcctc
    25141 acaagcatct acaaaagacc ttaccatgtt tatattccta tacagaacat tatattgtgg
    25201 gtcttgatgt gctccattga ctccttcctc agcaaacatt actgctactt caggaccagc
    25261 aacaccactt acatatgaat ggtaattaat aggtctacca acttcttctt ctataaaatc
    25321 aagagccttt ctttgagctc ttacttgttt tcttgttatt ggaactccac ctataccttg
    25381 gggagttcct tctataagcc catcaaagtg tgattgtcca gcagttctga taaccattag
    25441 atggtcagct ccatgatgag ctgccattct cattcttctt atatcatcct caaatctacc
    25501 agaagctatt tcagtagtaa taactggact tggttgaggg tctatattat tgaaatactt
    25561 agatgatggt agccctacac tcttctttaa aggttttgta cagtctttat atttaaaagg
    25621 tcccatttca agattttcca agtgttctct ccaaacccaa cctcttcgtt ttggtttata
    25681 tttatccaaa tcttttaata tatagtctat atccaatttt ttattttcct ttaacatgat
    25741 tctacccctt ctctaaataa acatgtaact tcatcccaat attcaccatt aactaataac
    25801 aaacctgctt ctctaaccgt tatattttta gatttagcca atttataaac tacatgccct
    25861 gctcctttac ctataagacc tctttccata accccttcta aaataggttt tacttcaaga
    25921 gaagaaaatc ccattcttaa caagacagac ctttctattg atggtgttgt attcttttta
    25981 ccaagttcta tcattggttc tactgtctgg gtagctaaac tccaaaatct ttcttttaac
    26041 tcatcatcac tcagttcttg aagatgtttt cttctaactt caaaatcatc ctctcttttt
    26101 ttcatgaaca ttcctccatt cgtattgatt taatcgaatt ttccgaattt ttattttata
    26161 ttaaattaaa aaacaataat tactataaac tatttaatac atcaacaaca aaatctcttg
    26221 aactttttac ttcttccatt aaaaattcta tatctcgatt acttagctta tcaatattat
    26281 caatgtgatt attagatata caatttttta taaatgactt tcttatttta ttcatatcaa
    26341 tatcaacaca tttcaataat tctggtcttt ctggtaatat taagtttact cctgcaattt
    26401 cttctcttgg atttccaaat tttatatcta ttccattttc tcttgcaaaa gtaagctgtg
    26461 gacatatatg tttacctgcg cctgtatatt ctgtttcttg taccactata actttatcct
    26521 catctaactc ttgagctaaa gagaatgctg ctgctaaaga tgtatttcct gctggacctc
    26581 tttctatacc ttctagctgt gccaataatt cagttgtata aaaaacttct ccttgtttta
    26641 ctaacacata tctatccata tatctaagtg gtctagctgc agacctaggt acatctgacc
    26701 tatcaggcca tgtacagtat ggtataccaa aacctgtatg tcctgttgta aatgattttt
    26761 tattaaattg attatcgcta gccatgtgta atccttttaa atctacactt gctcctacta
    26821 ctttagtatt aatagaacca gctcttatca atcctcttgc agttccagta agattaccac
    26881 cacctgcatt tgtacaaaca actatatcag ggtctttttt atacttttct ctaaattgta
    26941 tagctaactc ataacctaaa gtttctacac ctgcaattcc aaatggtgaa tataatgatg
    27001 cgttaaaata gcctgtttct tctaataaaa ctaagaacgt ataaaataac tctggtccaa
    27061 cagttaactg aacaacttct gctccatatg cctcacattt tctcgctttt tctataattt
    27121 caggttgacc tacaccatta gaatcataac attcttgaac tattatacat tttaatccct
    27181 gcattgctgc ttgtgatgct acagctgcac catagttacc actggtagct gctattacac
    27241 ctttatatcc catttgtttg gcatgatata ctgcagtagc tgctcttctt gctttaaaac
    27301 taccagatgc attcattgct tcatctttta caaatattct agctccttta ccttctttag
    27361 cacattttct agctaactta gtcaaatttt taagctctat tataggagta tttccaacag
    27421 catactgtga ttgaattact tcaatatctt gtaatgtata tcctgtttca ctcatcattt
    27481 tctcataatc aaaccctatt ccttcttgtt caaaagaaga gtaatctaat ccaattgcat
    27541 ttttcattat ttcgttattt cttcccataa cagcttgata actcatatct ttcattgatg
    27601 ttgaattatt cattatatac acctccaaat agtatttctt tagctttgat tcctatttgg
    27661 agtaattctg gtatacattt accataatca tgagtataat atggatttac tttaagtaaa
    27721 ttacctttta caagtctacc tgtaattgtt ttaacttcta ctaagtcgcc tatagaagca
    27781 tcactttgta taaaaccttt aacccacatt tctaaggata ctttcttagt atcctctgga
    27841 acttgtggag ctctttcttc tggagtcaat actatattgt gtataataac ccaatcatta
    27901 attttagcat ccattttttc atccccttat taattgacta ttgtcttact aattaattta
    27961 ctgttatttt aaattaacta ttattttacc aattagttag ctgttatctt actaattagc
    28021 ctttgattct tcttccaatt caacttctat ctgatttctt atatctccca ttattgctct
    28081 aggtactggt atatccaaca tagttttaag acctggtcta gcatttatta ttaatggaat
    28141 agagtttact atcattgcat aagtacctac tcctccaggt atttcaggat ttatttgtaa
    28201 atcaatgttt ggtatgcctt ttattgatac atagtctcct gttttaatac cttctgcttc
    28261 tggtattatc tgttgtggat gttccatttc aattaaaact tctccattta catatccata
    28321 tccacattgt ctacaaccag caacatttcc agccaatgcc tctccgtact tagattttct
    28381 atctactgta gtcattattg gttcttttgt ttgttctatc ttatctagtt tccaacctaa
    28441 tccatccgct atcatattta ttgattctac aaaacctaca tgacctgcta tagtcttatc
    28501 ttctacacct tttataaatt cttcccttgt tactccaaca ccttgttcca ccataacaga
    28561 ctttccaaat ggagataaat catttactct cttagctttt atactagtaa cttcttcaca
    28621 agtacctgaa agtgctaata ctagtaaatc taatacaaat cctggattta ttcctgttcc
    28681 aagtatactt actccgtttt cctttgcaac ttcatccatt tttttagcta aatctgcatc
    28741 atcagcttgt ggatatgcca tttgttctgc tgttgatata acatttattt ttctatttaa
    28801 taagaaaatt attttatcaa aagccttttt agtaaatgaa tcagttgcta acaaaacaac
    28861 atctacactt ttttctctaa aaacttcttc ataattttta ttgataataa cttctggtct
    28921 ttctcctctt tcaattccta agacatcata catacttttc cctgaagtac ttctactaca
    28981 aacagatact atttctatac cttctttttt tagaatcata ttagccatac ctattcccat
    29041 agctccaaat ccccatattc ctactcttac ttttctcata tatacaccct cctaaaaatt
    29101 ttatatattc tcaataatta gttaaagcat aaatcgtgcc aactttaaaa ttaaagtaaa
    29161 tatttattgg aatgttgaaa ctataattat tttattatgt attttcataa ttaaaaaata
    29221 atatttcttc tgagaaataa aaaaagagca aaatattttg ctcttttata ataattttgc
    29281 tcctttattt tgctttttta ttttactttg attttgcttc tattttactt ttttatcttg
    29341 cttatttaca atattttgat attatatttt tttattttat actgtaaatt ttgcctattc
    29401 atacctaaaa ttgatgcagc tttagttatg ttgtatgatg ccttttctag tgattcatat
    29461 atatactttt cttctatctc actgataatc ttactaagtg ttttgtcctc caaatcgttt
    29521 aaagaataat tgttactctc tttattctct ttaagattgt aatagtcgtt tagattaatc
    29581 atacttgttt ctatttgtac ctcatcttcc atcatactca tagtacaata aattatgttt
    29641 tctagctctc ttatgttacc ttgccaatcg taatttttta aaattttaat agcttcatct
    29701 gaaattccta ccactttttt attcaacttt gtattatact ttgaaataaa ttttttgcac
    29761 aatagaggta tgtcctcttt tctatctcta agaggaggaa tttttatatg aattatgtta
    29821 agcctataat ataaatcttt tcgcatttta tgctcgttta aaattacctt tggactttca
    29881 tttgtcgttg caataattct tacatctata ggtatatcct tagttcctcc aacccttctt
    29941 acataacctt cttgtaaaac ccttagcaat ttagcctgta acataatagg catagaattt
    30001 atctcatcta ataacaatgt tcctttgtta gcttgttcaa ataagcctgg cctatctatt
    30061 gctcctgtaa atcctccctt gctagtccca aaaaggatac cttcgaatag tgattctggc
    30121 aaagctgcac aattttgtgc tataaatgga tagtttctcc tattacttcc atagtgaatg
    30181 gactggctaa ataactcttt acccgttcca gtttctccat atataaatac tgaagcatca
    30241 gacttgcaag ctttcttaga ttttgctatt gcttctttaa tcaaattact ttcccctaaa
    30301 atatcatcaa attcatagcc ctttttttgt ctactactaa ctttattgac taaatttttt
    30361 ttataatttc tgtttagtaa attttctatc gttgtcatat tctttgatat ttcaagtgcc
    30421 cctacaaact cgcctttaat atcaattggt attgtagtat taattgctgt tatctctttt
    30481 ccatttttat ctagatattt ttgaatattg tctttgataa cttccctttt ttccaaaact
    30541 tctaatagtg tactcctgtt tattttcatt ttgttgacta tatcaaaaaa tgaattggtt
    30601 aaaatatcat gtctgttcat tttttccatt ttttgtacag aattattgta gattatagtt
    30661 ttcccgtctt tattaacaaa atgtacccct tcatcaatat tttccaatat ttcattcatt
    30721 attttaaaaa atatctcttg gttcaactat atcaccccag tacctaaata tataaaaacc
    30781 caaaaaatta ataaaatgtt gctattgtat ttatcttatc atatatt
//
LOCUS       DAELJO010000022        30136 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELJO010000022 DAELJO010000000
VERSION     DAELJO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.022"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(164..769)
                     /locus_tag="KPB88_000930"
     CDS             complement(164..769)
                     /locus_tag="KPB88_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0053228.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(894..1011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1071..2237)
                     /locus_tag="KPB88_000931"
     CDS             complement(1071..2237)
                     /locus_tag="KPB88_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053229.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2385..4526)
                     /locus_tag="KPB88_000932"
     CDS             complement(2385..4526)
                     /locus_tag="KPB88_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG0053230.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4670..5777)
                     /gene="prfB"
                     /locus_tag="KPB88_000933"
     CDS             complement(join(4670..5701,5703..5777))
                     /gene="prfB"
                     /locus_tag="KPB88_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG0053231.1"
                     /translation="MLKLQEYRHGMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5792..8467)
                     /gene="secA"
                     /locus_tag="KPB88_000934"
     CDS             complement(5792..8467)
                     /gene="secA"
                     /locus_tag="KPB88_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0053232.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8599..9150)
                     /gene="raiA"
                     /locus_tag="KPB88_000935"
     CDS             complement(8599..9150)
                     /gene="raiA"
                     /locus_tag="KPB88_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0053233.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9703..10377)
                     /locus_tag="KPB88_000936"
     CDS             complement(9703..10377)
                     /locus_tag="KPB88_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG0053234.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNIMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISI"
     gene            complement(10380..11432)
                     /locus_tag="KPB88_000937"
     CDS             complement(10380..11432)
                     /locus_tag="KPB88_000937"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG0053235.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIKIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11481..12440)
                     /locus_tag="KPB88_000938"
     CDS             complement(11481..12440)
                     /locus_tag="KPB88_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG0053236.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12536..13054)
                     /locus_tag="KPB88_000939"
     CDS             complement(12536..13054)
                     /locus_tag="KPB88_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG0053237.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13065..14399)
                     /gene="celB"
                     /locus_tag="KPB88_000940"
     CDS             complement(13065..14399)
                     /gene="celB"
                     /locus_tag="KPB88_000940"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG0053238.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14438..14764)
                     /locus_tag="KPB88_000941"
     CDS             complement(14438..14764)
                     /locus_tag="KPB88_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0053239.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14788..15096)
                     /locus_tag="KPB88_000942"
     CDS             complement(14788..15096)
                     /locus_tag="KPB88_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0053240.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15237..17150)
                     /locus_tag="KPB88_000943"
     CDS             complement(15237..17150)
                     /locus_tag="KPB88_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0053241.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKEFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17482..18264)
                     /locus_tag="KPB88_000944"
     CDS             complement(17482..18264)
                     /locus_tag="KPB88_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG0053242.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18267..20486)
                     /locus_tag="KPB88_000945"
     CDS             complement(18267..20486)
                     /locus_tag="KPB88_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG0053243.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20750..21847)
                     /locus_tag="KPB88_000946"
     CDS             complement(20750..21847)
                     /locus_tag="KPB88_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053244.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22163..23356)
                     /locus_tag="KPB88_000947"
     CDS             complement(22163..23356)
                     /locus_tag="KPB88_000947"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG0053245.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23493..23596)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23785..24294
                     /gene="yyaC"
                     /locus_tag="KPB88_000948"
     CDS             23785..24294
                     /gene="yyaC"
                     /locus_tag="KPB88_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG0053246.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24405..24833)
                     /gene="fabZ"
                     /locus_tag="KPB88_000949"
     CDS             complement(24405..24833)
                     /gene="fabZ"
                     /locus_tag="KPB88_000949"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG0053247.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24849..25868)
                     /locus_tag="KPB88_000950"
     CDS             complement(24849..25868)
                     /locus_tag="KPB88_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0053248.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26065..26325)
                     /gene="spoIIID"
                     /locus_tag="KPB88_000951"
     CDS             complement(26065..26325)
                     /gene="spoIIID"
                     /locus_tag="KPB88_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG0053249.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26423..27091)
                     /locus_tag="KPB88_000952"
     CDS             complement(26423..27091)
                     /locus_tag="KPB88_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG0053250.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSIGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27157..28221)
                     /gene="spoIID"
                     /locus_tag="KPB88_000953"
     CDS             complement(27157..28221)
                     /gene="spoIID"
                     /locus_tag="KPB88_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG0053251.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28308..29561)
                     /gene="murA"
                     /locus_tag="KPB88_000954"
     CDS             complement(28308..29561)
                     /gene="murA"
                     /locus_tag="KPB88_000954"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG0053252.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29582..>30136)
                     /locus_tag="KPB88_000955"
     CDS             complement(29582..>30136)
                     /locus_tag="KPB88_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG0053253.1"
                     /translation="NKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKGSDK
                     EISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVGEYT
                     KRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKINLNI
                     GIKYNEDDGKTLIYIATPIIKLDY"
BASE COUNT         8964 a         5681 c         3178 g        12313 t
ORIGIN      
        1 tattttaaaa tttaaatttg tctaaatatt tcttatttta tatacatttt ttgataaatt
       61 aataaagcca taatatattt aatatatcaa tcacaatata ggactgaagt tataaataaa
      121 ttatggctat tcaatacatt taattttata ttttcttgtc taattatttc tttattatat
      181 ttcccatttt tttataaagt ggcaatgtta ctaaagatat tataacagcc ttcagcacat
      241 taaatggagc tatcatccaa attacaaaag tgaataaatc atgaacttga ggatttatag
      301 ctgaacctaa acctattata gcatctagtc ccataagttg accataaagt ggcaacatta
      361 caaaataatt tactaatgaa ccaattatag tcatagatat tgttcctaat acaaaaccaa
      421 tcagtacacc ttttaaatct ccacttctct tatatgaata acaaagtatc attacataag
      481 aaccaccaat taatatattt gcaaattctc ctattcctcc agttgttgtt gctgttattg
      541 cttgtaacac attttttact acaactattc caaatccaac taatggtcct agagacattc
      601 ctccgaatat tgctggaata tctgataagt caatttttaa gaaatctgga aatatacctg
      661 gtattggtgc ttgaataaac attaataaat aagatattgc agataaaatt gacattacta
      721 ctaaagttct tgttgaaatc atattgcctc tttttgctgt gttttgcata actattttcc
      781 ccctaatcaa tatgtttata ttttttagga aaaacaaaaa actcgaagac cctatcttcg
      841 agtttttatg gatatagttt aaacaaacta aaataaactt atttatcttc cataaatatc
      901 ttctctcatc cagactttac tgtcggcttt ggaatctcac caaatcaacc acctaagtag
      961 ttcgcgggct ataccgccgg tagggaattt caccctgccc cgaagatctt ctttgatttt
     1021 ttaattttcc ttacactctt atattagtta attttttaat aattgtcaag ttattttttc
     1081 tcatacacta ctttgccatt tattatagtg taaagtacat tactttgaat ctctagtgga
     1141 gaattatccc aaattactag gtctgcatct ttaccaactt ctatagagcc tactctatct
     1201 tctattccta aagtcttagc tggatttatc gtaattgatt ctattgcttt ttcttctttc
     1261 ataccatgtt ttactgcaat acctgcacat attggaagat attgaactgg tattactgga
     1321 tggtctgtca ttatacatac atcaagacct gcatttgaaa gtattcctgc tgtattaaat
     1381 gtcaaatttc taagctcaaa tttagacctt tctgacaaag atggccctac tattacagga
     1441 aacccttctt ccactaattc atctacaata agatgacctt ctgtacaatg gtctaatgta
     1501 agctttaaat taaattcttt tgcaattctt attgctgtaa acatatcatc tgctctatga
     1561 gcatgtgcct taaaaggaat ttctcttctt aaaactggta ttaaactttc catttttata
     1621 tcatattcag gtttttcaca gtcttcatca tcatgactct catacatatc aatatcctct
     1681 aaatattctt ctgctttttt taggttttct ctaagtaaag cagctatagc cattctagtt
     1741 tgaggagatt tatcatcctg accataacag ctctttggat tttctccaaa agcaatttta
     1801 gatgcaactg gattttttat taccatttta tcaattcttt ttccacatgt ctttatagct
     1861 atacattgac cacccataac atttgcactt ccaggggttg tacaaacaga agttattcca
     1921 ccttcaaaag cttctttaaa agttctatcc attggattaa ttccatctat aggatttaat
     1981 tgtggtgtta ttgggtcagt ctcttcattt ccatctgctc cttcaaaccc tataccatct
     2041 tcccataagc ctaagtgagt atgagcatct ataaatccag gaaacacaag tttattacta
     2101 gcatctatta cctctacatc taaaggagct atcaaatctt ctccaatttc tattattttt
     2161 ccttcatcta taagtatatc tccatgaatt atcccatttg ttatggtatt tattttacca
     2221 ttttttataa aaatcattca ttttcctcct gattttcaag actatcgttc ttattttaat
     2281 atattttctt actatatact tttacaaatt tttaacattt atacactcga gtaatcttat
     2341 ttacaaatat agaatctaag tactataacc atacataaac ttatctattt tttcattgat
     2401 aaggcaactc tttttttgtt taagtcaatt ccaatgactt ttacatctac tatatcacct
     2461 actgtaacaa tactcatagg gtctttcaca aaactattac tcatttctga tttatgaact
     2521 aatccgtcat tttttattcc aatatctaca aatgcaccaa agtctacaac atttcttatt
     2581 gtacctttta aagtcatacc ctcttgtata tcttcaattt ttaagacatc agttctaagt
     2641 ataggtttta taccttcttc tcttgggtct cttcctggtt ttttgatttc agctatgata
     2701 tcttttaatg taacttgtcc tacttccagt ttttcactta attccctcaa gccaatttct
     2761 tttatttttt catctatttc acctatattc ttattcttta catcatctaa agaatatcca
     2821 attatctcaa tcattttttt acatatatca taactttcag gatgcacacc tgtattatct
     2881 agtggatttt taccctctaa gattctcata aatccagcac attgagtaaa tgcttgaggt
     2941 cctagtcttt ttactttttt caattgtgct cttgacgtaa aatctccatt ttcttctcta
     3001 tatgctatta tattttttgc tatagcttta cttattcctg caacatgttc tagtagggag
     3061 tatgatgcag tatttaaatc tactccaaca ctatttacag aatcttcaac aactccatct
     3121 agtacttctt ctaatctctt tttgtttaaa tcatgctgat attgaccaac accaatactt
     3181 ttagggtcta tttttacaag ctctgctaat gggtcttgta atcttctagc tatagatata
     3241 gctcctctta ttgatacatt tatatctgga tgttcctcat ttgcaagttc agatgctgaa
     3301 taaacagatg ctcctgcttc actaactatc acatactgca cttcgctatc tatttctttt
     3361 atcatttctg atacaaaagt ttctgattct ctagatgcag taccatttcc aattgaaatt
     3421 atatctatat cgtatttttc aatcaatcct tttaaaactt tctttgctcc ctctacatca
     3481 ttttgagggt ctgttggata tactgttgta taatctagta gtttaccatt tttatctact
     3541 acagctattt tacatccagt tctaaatgca gggtcaaatc ccataactac tttgtcttta
     3601 actggtggtt gaagcaataa acttttaaca ttctttccaa atacacttat agctctttct
     3661 tgagctattt ctgttaaatg atttcttatt tctctttcta ttgatgggaa aattaatctt
     3721 ttataagaat cttctattga acttacgatt tcttctttat ttttgaaatt tttatcattt
     3781 acatattcgt ttattatgta atttaaaact ttatcattat ttatttccaa cttaaccttt
     3841 aggaagcttt ctttttctcc tctgtttatt gccaaaactc tatgtggagc catacttttt
     3901 actgcttcgc tataatcata atacatatca taaaccgatt tctcatcagt agcacttttg
     3961 ctaactatca taccttctct taaagctaat tctcttatat actttctaat cttagcatca
     4021 tctgagacta gttctgcaat tatatctcta gcacctttta gagcatcttc aatagaaaga
     4081 acttctttct cttcatcaag ataattcttt gcctctattt ctatattatc taaattattc
     4141 tctagtattg acaatgctaa attttctaat cctttttctt ttgctattgt tgctctagtt
     4201 cttttctttt gtttatatgg agcatatata tcttccactt cttgtaatgt ttttgctttt
     4261 tctatatttt gtgttatttc atctgttaat ttcccttgtt catctattag tctaactaca
     4321 tcatcttttc tactttgaag atttctcaaa tacattagtt tttcataaaa ttctcttagt
     4381 gtaacatcac tcatttcgcc agtcatttct tttctatatc tagctataaa tggtattgta
     4441 tttccttcat caataagttt taatgtatta tttatttgtt catctctaag attaaactct
     4501 ttttttaaga tttgatttat atccaattta ttcactccta atgttagttt ttcataagta
     4561 taacaaaaca gaacgaaggc tgagggttat cgtcctcagc ctaagtgctg taagtgcata
     4621 ttaagcacta accttcttat acatatatct taattacagt gcatatttat taaactattt
     4681 tattcatttt taagtattca ttaataaaca agtctatatt tccattcatt acactatcta
     4741 cattaccaaa ctcagcattt gttctatggt ccttaaccaa tttatatggt tgaaaaacat
     4801 atgacctaat ttgactaccc caagttattt gtgagtattt gccttgtata tcttctattt
     4861 tttccttttg ctctaactct tttaattcta tcaatttcgc cattaaaagt ctcatagctc
     4921 tgtccttatt tagatgctga gacctttcat tttggcactg aactactaca ccagtaggga
     4981 tatgtgttat tctaactgct gaatcagtcg tatttacatg ttgccctcca gctccagaag
     5041 ctctatatgt gtctatcttt aaatctgatg gattaatttc tacttctata ttttcatcta
     5101 actctggtgt tacatctata gatgcaaatg aagtatgtct ttttcctgat gggtcaaatg
     5161 gagatattct cactaatcta tgaactcctt tttcactctt taaataccca taagcatttg
     5221 ttccttctac aagaattgta gctgtcttta tccctgcttc agggtctgat attatatcta
     5281 aaagcttaac tttataaccc ttactttctg accatcttat atacattctc aaaagcattt
     5341 gagcccaatc ttgagcatct aagccaccag taccagcatt tatagataat atagcattat
     5401 ttttatcata ctctccattt agaagagtct ttattttcat ttcagataat ttttcttcca
     5461 ttgattctat ggacttttct atatctcttt ctacagaatc atcatcttct tctaaaccta
     5521 tttcaattaa tacttctata tcttctaata agctttttaa gctttcatat tcttctaatg
     5581 tctcttttaa agatttattt tcttgaagta ctctttgagc gacttcatta tcattccaaa
     5641 agtcttgttg attcatcttt tcttcattaa tttgtatatt tttcatcaat gaagctatgt
     5701 caaagagaaa ccctcaattc ttctaaattt gaagccatct gctccatacc atgtctatac
     5761 tcttgtaact ttaacattta atcctccgag attatcttcc acaacagttt ttatattttt
     5821 taccacttcc acaagggcat aagtcatttc ttccaacctt ttcatctttt ttaacagttt
     5881 tacttgttgg aagtgttcca tctgatggag aagatagatt ttcaacatct acaactgctt
     5941 ttctttccac tggagtttct attgttatat taaataaata tcttacagta tcttctttga
     6001 tatgtttatt catttcatca aacatatcaa aaccttccat tttataagca ataactgggt
     6061 cttgctgacc tacagcacga agaccaatac cttggcgtaa ttggtccata gcatctatat
     6121 ggtctatcca atggttatca acagcttgaa gtaagattac tctctcaact tctctcattc
     6181 tctcatagcc aacttgttct tcttttgaag tatatatctt catagctatt tcataaactt
     6241 tttctgttat ttcaactgga ttaagttttt ctatttctgg tatttctatg ctaccttttg
     6301 gcaagaatag attatacata tgttctttga atccttcttc atcaaatcct ttatcttgtg
     6361 tataaagagt aactgcttct tctattatag aatgagtcat tgattgtatt tgttcttgta
     6421 aatcttcacc ttctaataca cgttttctct ctgcatatat tatttctctt tgtttattca
     6481 taacatcatc atattgaagt acatgctttc ttattccaaa gtttttacct tcgactttct
     6541 tttgtgctcc ttcaatagac ttagataata ttctatgttc tatcggcata tcttcttcaa
     6601 gacctatttt atctacaata ccagatattc tatcactacc aaacaatctc ataaggtcat
     6661 catcaagacc tatatagaat cttgaactac ctgggtcacc ttgacgtcca gcacgacctc
     6721 tcaactggtt atctattctt cgagattcgt gtctttctgt acctattata gcaagtccac
     6781 ctgcttctac aacttgtttt tgttcttctt gtgtttgttg cttaaacttt tcaaatagtt
     6841 tttcatactc ttctctagct tcaaatagaa tttcattacc ttttacaggt ataccttcta
     6901 ttggagtatc tactctattt actatttctt ctttaaatcc atttcttctc atttctctct
     6961 tagttaaaaa gtctggattt ccacctagaa ctatatctgt accacgacca gccatattag
     7021 tagctattgt aacagctcca agtctaccag cttgtgctat tatttctgcc tctttatcat
     7081 gatgcttagc atttaatacc tcgtgcttta tgcccttttt ctttagtatt tgtgataata
     7141 attctgattt ttctattgat actgtaccta caagtattgg ttgattgact ttgtgccttt
     7201 caattatttc ttgtgctaca gcattaaatt tacctatttc acttttgtat acacagtctg
     7261 gtaagtcttc ccttatcata agcttattag taggtacttg gaaaacatcc attttgtaga
     7321 ttgctttaaa ctcttcttct tctgtttttg ctgtaccagt cataccagaa agttttttgt
     7381 acattctaaa gtaattttgg aatgttacag tagctagtgt tttagattct ctttgtattt
     7441 taagaccttc tttagcctct attgcttgat gtaaaccttc tgaatatctt ctaccaaaca
     7501 taagtctacc tgtaaactca tctactatta ctatttctcc atctttagca acataatcaa
     7561 catctttctt cattattaca tgtgctctta aagcttgatt tatatgatgg tataattctg
     7621 tatgagatat atctgttata ttatcaacat taaaatacac ttctgctttt tgtatacccg
     7681 atgctgttag agatacagcc ttatcttttt cttctaattc ataatcatct ggttttaagg
     7741 ttaaaacaaa tgtgtttgca tctgaatata agtgagttga tttgtctcca ggtccagata
     7801 tgataagtgg tgttctagcc tcatctatta gtatagagtc aacctcatcc actatagcat
     7861 aatttaatcc tctttgaact ctttgctctt tgtgtattac catattgtcc tttagatagt
     7921 caaatccata ttcattattt gtaccataag ttatatcaca atcatattgt tcttttctaa
     7981 cttttggatt ttgaccatgt ataataacgc ctacactcat tcctaaaaac tcatagattt
     8041 ttgccatttg gtctctatca cgttttgcaa ggtaatcatt tactgttaca acatgtacac
     8101 cttttccagt aagcgcattc aaataaacag gagcagttgc aaccaaagtt ttaccttcac
     8161 ctgttttcat ttctgctatt cttccttgat gtagtactat acctccaatc atttgaactc
     8221 tgtagtgtct aagtcctaac actctcttag aaacttctct aactactgca aaagcttctg
     8281 gaagtatatc atctatactt tcaccatttg ctaatctttc tttaaatata tttgtcatat
     8341 ttttaagttc cgagtctgcc atagattcaa atttaggttc taatgaatct attatatcaa
     8401 cagttttatt gaactttttt aattcttttt tatctgccat gttgaataaa ttatccataa
     8461 agctcatatt ttacatctct cctcgttctt tattgtacta ttattcattt taccataaaa
     8521 actatacttt taatattaat ttaaaaagct atcgataatt tatttatcga tagctaatat
     8581 gattaaatct aaattgcttt attcatgttc tattatacca tatccaccat tattacgctt
     8641 ataaactact gctatttcat ctgaatctat gttcttgaac atataaaaat catgtcctat
     8701 taattccatc tgtaatacag cttcttcttc actcattggc ttaacactaa atttcttacg
     8761 tctttgaatt actaattctt gattttcatc cccatagtcc tcttgttcat cagctagatt
     8821 tatctctgca tcttggaatc ttatactctt gttgtcttta tgtttatctt gtaatctctt
     8881 tttatatttt tttaattgtt tacttaattt atcatataca acatctatag ctgtgtataa
     8941 attttcctgc atatcttctg ctctaattat aggaccattt acagtagcta ttgttacttc
     9001 tattttttgt cttgcttttc tcgcacttac tgtaactttt acatcagtgt ctggatgaat
     9061 gtaattatct aacttactta gtttagtttc gattttattt tttattgcat cagttaattc
     9121 gatttgtttt ccagatataa ttatgttcat aaatacatct cccttcaatt atgccagtat
     9181 aagaaaattt atactttaat aatatattct acacgaactc tcataatcct ctttttatta
     9241 tttactttta tgattttttc tattcaattg atagaattat ttatcatttt tagttacatt
     9301 gatacttatg tggattaata ttattacaaa cacctgtgga taatgtggat aaaactgtta
     9361 ataactcttg tttttgttta tttaaagcta tttttactcc tatttctatc cacaaaaatc
     9421 tccattaatt gtttattaat atgtgaattc atgtcataag atggtaattt taggaaatta
     9481 tgtgtattat atgccaatat acctttataa ttttttaata aatataaacc ctctaaaacc
     9541 tccataatct atcttttctt tcattattta tatttaaaac tacttaaaag ttataaagca
     9601 tactcaacaa taatttcata tttaatacct attaacattc atatacccaa attataatta
     9661 acttactctc tttatttaaa ctaagtattt tatattcata tactatattg aaattatatt
     9721 tacgagttgt ttaagctttt gtctatctat atcttcaaaa ggactgttat ttattctaat
     9781 cgctgtacca aaatgaaaat tacttgcctt agttaattcc tttatctttt ctatattatt
     9841 aaaattaaga cctccaccta aaagaatttt tatgtgatta gaactaagca tcatattctt
     9901 tatcatttgt atgttatcaa ctatacttcc tttccctcct gatgttaata tattcgtaat
     9961 tttatcataa tccttcaaaa ttttaacact atctattgta tttgattcat ctatggctct
    10021 atgaaatgta acatcaagat tatcacaaca tttcaataaa acatttagat ttttctcatc
    10081 tatattattg tttttatcca atacaccaaa tacaactcca ttagcaccaa tctctttaac
    10141 cacacctata tctttttgca ttatacttat atcttcttta ctgtatacaa aacttttcgc
    10201 atgatgtctt atcattatgt tcacaggtat ctttacactg ttaacaacac tttctataag
    10261 accaaaactt ggtgtcaatc caccttcagt taaagcacta actaactcta ttctatctgc
    10321 accacaatct tctataattt tagcgtcttc tactgacatt cctattattt ctaacataac
    10381 tactcctttg ctaattcata tattgcattt gcaaatatct ttgcatttaa tattatatct
    10441 tctatatcca tgtattcatc aggattatga gctattcctt tttgtcctgg aaatgagggt
    10501 ccaaacggaa ctatatttgg cattactttt gcatacgtac ctccattagt tgttactgga
    10561 gttccatcta atgatgtaac cctttcataa gctagctgaa gctttttaac aagattactc
    10621 tctttatcaa aatatacagg attaaaatta gaaattacct ctatatcgat actatttgga
    10681 aaactagctt ttatttcatc taatacatct ttcaatttat aactaaaatc gtattttacc
    10741 tttatatcta atatagtatc actattttct atattcagct ctttatatgt aacaagcaca
    10801 ttttcagaca tattttctat aaaacctagg tatacatctt cttcataatt gattttactt
    10861 tttattttaa tctttgccat gcctttttca ccatatacaa caggatattt acaatctggt
    10921 gtaaatccca taataggtgc tttttctttt tctaaataat atggtatatc ttcaaatcca
    10981 ctttcttcat ttgtaccaaa tattattcta actctcttat ctaattttaa gtttaattct
    11041 ttaatagcat ataatccata taaggcagac attataggtc ctttattgtc tagtactcct
    11101 cgtccataaa ttcttccatt agtttcttct cccttataag gctgatgctt ccaaccatct
    11161 ccttcatgta caacatctac atgtccaatt acacaaacat aatcatcact atctccatgc
    11221 tctgcataac caatataatt atccaagttt ctaaccttaa atcccaattt ttctgatatt
    11281 tctaatgctt tattaagtgc ctcccctact ttttcgccaa aagggaagcc attttctgac
    11341 tcccctttga cactaggtat ttttacaata tctataatat cacttaataa atcttctttt
    11401 aactcatcta ttttattact tatgatattc ataaaaatct ccttattata aaactctatt
    11461 ttaattatgt aaccactact ttactcactt acttctattg gtgcttttat tctagcctca
    11521 tatgcttcca tccactcatt tgatataacc ttatgtatag tctttccatc tactctattt
    11581 gaaatatata cagtaggaga ttcttcttta gttactactg aaaaagtaaa atcagctata
    11641 tttgtagcta ctccccattc acctttatta ttcatggcaa ctaatgataa atcaccagct
    11701 tttccccttc tttcgataag ctgtttatca agttcattta ctgcaatatc acatgcttct
    11761 tgtggatgct tcccttcttt cataagtcta actatctcat aagatataca tcccttcatt
    11821 aaatcttctc ccaaaccagt agctgtagct cctcctatac tactatcaac atagaatcct
    11881 gaaccagata gtgcagaatc tcctattcta cctcttttct tcataaacaa tccacttgtt
    11941 gatgtagctg cacacatttt tccagttttg tctaaagata tcataccaac agtatcatga
    12001 ccagcatatg ggcttaatcc tttgtctaaa gtctcttttt ttctcttttt ataatgtaac
    12061 ttcgctctat cagtcaacat attttttctt tcaaatccat ttttatgagc ataatcttca
    12121 gcacctattc caactaaaaa attatttacc ttctcataac ttaacttcct agctatgctg
    12181 acaggattag aataatctct tataccagca actgctccaa tatctaatgt gttaccatcc
    12241 ataaatgcag cgtcaagttc tacctcacaa ttttcatttg gaagtccacc atatccaacc
    12301 gatttataga atggatagtc ttcaactctt cttacagcaa gctctacagc atcacctgct
    12361 tccatgcctt tttctaaatg ttctgatgcc tcagttattc cttctagggc cattctccat
    12421 gtcgctatca ttccccacat gttaaaattc ccctccttaa attatataga ttttactaat
    12481 gtatgcttat tataaatagt ataatattta aacttgctaa taattaatac tcttattaat
    12541 aacaaaattc ttctttcatc aaagttatat cttctttatt tgctattgct tttgccaatt
    12601 tacatatgtt atttaatgaa tcattaagac caattccacc ttttttggga gtcttaacta
    12661 tacatatgtc atttttatat tcgtttatca catctatatt ttcttctgac atagctgcaa
    12721 aagctggtat atctgtttta tcatttatat ttttagacag tattgctgac atcttagaaa
    12781 agttttcgta attaaagctt ggtccacaga ttactacatc agggtttaat ttttttacca
    12841 tagctatcat cttactcgtt acttcttctt gattatttac aaaaaattca tccccacaaa
    12901 ataaggtagc tattacctta gattcattta aaaattgctc catcattaca cctggtccta
    12961 atgggcttga ttttccagca ggaggtatat tacttttctc ttttcctcca gctccagctt
    13021 gtatttggtc caatatcata actattttct tcataatctt cctcctataa tactaaatca
    13081 tcaaaagata tatcatcatc ttcctcttca acagtttcat tttcttctct caagtattgc
    13141 ttatctagca tttttataaa tggtatatac ataaatattc ctagtataag aagtactaat
    13201 tgtaagactg caccttgcca tcctgttgtt aaaaaaccag agaatataat tggagttgtc
    13261 cacggtaact gaactccatt tgtaagaggc actaatcctg ctgacataca gaagtaagct
    13321 attattatat ttattgttgg aactattgca aatggtatca atagtattgg gttaagcatt
    13381 attggtagac caaatatcaa tggctcattt ataccaaata ttcctggtaa aattgatagt
    13441 tttcccattg actttatacg tttactttta catattaata acatagctat gactaatgat
    13501 agtgtactac ctgctccacc aaatgtcgca aacatatctt ggaattgacc tgttattatg
    13561 tttggtattt ttgaaccagc ttgaaaagca gctaagtttt cagctgatag aatttttaat
    13621 attggattat acacagcccc aacaacacta cttccattta ttccaaagaa ccagaataaa
    13681 tgtaagaata tataagcgat tgccatagca cctaaactat ttcctaattt taataatggc
    13741 atttgtaaaa acttaaatat aaattcatgt atacttccat atggtgtagc ttcaaaagct
    13801 atcttaacta aaaagaaaac taccataact attgcacttg gtattaatgc tgcaaatgat
    13861 tttgatacag ttggaggaac tccatctggc attttcaatg tccatccttt ttttacaact
    13921 gcagcaaata attttacaga ggttattgct gttatcatac ctacaaacat tcccttagaa
    13981 cccatccaac ctagtggtat acctgttacc atgtaaactg attctgttcc ttctggtgta
    14041 tatggtatta caaatggtgt tattataaaa aaggcaacta ttgcaactgc tgctgatgct
    14101 attgggtcta catctatttg ctttgcataa gaatatccaa tccctaaaac tgcaagtaga
    14161 gtcattactt caaatgttgc tacagttggt tgttgcaatt ttacagccca atctggacca
    14221 aaaatttgac ctacaaattc attccatcct tttataggaa aatttgctac taataaaaat
    14281 aatgaaccta ttattaataa tggtgatgat actaaaaaac catctctaat tgcaattaat
    14341 aatttatttt tacctatttt ttcagccaat ggcataagta ttctttccaa tctattcatc
    14401 tatccaccct catttcctat tattttttac tataattcta cgcttgttta gattttatta
    14461 gctttatagc aagttttaat actttttccc cattcatagt tccataatca acttcattga
    14521 ttatatctat aagttttcct tgagcttcaa actttggttt taactccttt agcatgtatt
    14581 taacttgtgg tcctaataat atacaatctg gatttttttc ttcaactatt ttgtcaattt
    14641 ctgctatcgg aaatgcctct acttctattg gtaaattgtg ttcatctgct accttctgca
    14701 ttttatctgc aagtaaactt gttgacatac caaagctaca aaataaatat acttttcttt
    14761 tcatttttta aatccccctt aattttttta ttttaatttt tgaatttctt ttcttaacat
    14821 aatcatttct tttataaggt ttctctcact catacaagtc attaaatggt cttgagagtg
    14881 aatcaataaa atagatatat ctgatttatc tcccccagct tccttttgta taagttcagt
    14941 ttgtacttta tgtgctttta aaatttcttc atttgcattt tcaattaatt catctgcttt
    15001 tttaaaatcg ttttcctttg catagtcaag tgcttcataa agcatagatt ttgcatttcc
    15061 agcatgaatt ataatcttta aaactatttc ttccattttt tcctcctaag ttgcttctct
    15121 acttttagat tatttatacc ttacaaacat ataatatctt ataaaaacaa aaaaacatgc
    15181 acaacttttt ccatcacatg gaaaattttg tgcttatcca tagttcacta tgaattttat
    15241 ataagaatat cttttacctt ttcaatgtca catttactta gaatatctag catattttca
    15301 tcctctgtta aattagatat ttctttaaat aattctctaa attcttttct atcatcatct
    15361 tttttatatg ctaacaaaag tataatcttt atatcctttc catttctaat ttttattggt
    15421 gtttttaacc ttgcaaaaca taaaacagat tgttttatat atttcatgtc tccatgaggg
    15481 aataatattc cttttccaac attggttgga tatgactttt ctcttgttat aatagattgt
    15541 aaatactctt tttctgcata atgtttttca attaataaat tagttaaata tgttatagct
    15601 tcataaatat catcatgttc taactctctt attaaataat cagacatcat taatctgttc
    15661 ataagtttat atgaagtgtc tttttctttt attttgtttg tgaactcttt aagtttattc
    15721 tcatcctcat cagtaaataa tggtgttgtt ataaaaattg gtttttggat attttcatac
    15781 tcttgaaatg caattataaa atctatagtc ttattaaagt tatctctttt taaatcttcc
    15841 tctcttaatg tttcaataat ttttatgtta tcaaatctct tttctatctt tacttttaat
    15901 agcatactta ctccaaaact aaatggacat acaatacttg cctttttatt tgattccctt
    15961 ttgctgtatt ttctttctag agaggtttga aagtgaagtg taagataacc aatttcatct
    16021 tccgttattt gttttccttt taattcaatt tctattgaac ttgatattac tgtaaataaa
    16081 aaacaaaatt tagtttttat gttatctaaa agtggatttt ctaagtaaac attgcttttc
    16141 atttgatgca ttgtaacatt caaatgacat attaattgtt cataaagtaa tatatcatta
    16201 ttaaaattta ttcctgattc ttctgatact aaatttataa ttttttttgt ataacttgct
    16261 aattcttcat ttagatataa agatttctta atattattac tatttatttg tttgttcata
    16321 cccaatatta aagattctat aaacaatttt tcttcataat ttaatactat ggataaatta
    16381 gatttcaatt catcttctat atccaatata agcattttta gcttaagctc tttcttagta
    16441 tttgatactt ttaaagaatt tcccagcctt attcgtatta tatttataag caaaaatagc
    16501 atcaattgct tgaatgatat atctgtaagt cttatatcca tatcctcctc tatagtaagc
    16561 attatgtttt ttacaattat tatatctact atatggaagt actctatttc atctatgttt
    16621 aatctgtatt tatctatata tttaaataag aatgatacta acattattct tatgtctttc
    16681 tcatcccctt caatatatat accactgcct tttttagtcg agatatttaa attataatta
    16741 tttaatgcca ctgataattt acttatttct tctctgacaa tatttttatt tatgtacaac
    16801 ttattagata aatcatttaa agtaactttt ttattattac aaagtagtaa atttaatatc
    16861 tcaacttgct tatgaagctc actatttact cttttaactt cacctcgcaa cttctgattt
    16921 accagtattt tgtctgttcc atctccacta aatttaatcc caatattagg ttttcttttg
    16981 atataagcat tacaaaaagt agataaccat tcatcaatat atttgcaatc atttcttatt
    17041 gtcctttctg aacactcaaa atcattggca atcttattaa catttataaa ctcttctttt
    17101 aatagcaatg ttgttaataa attttcttgt cttttattca aacatatcac ctctttatga
    17161 ctcaacctaa atttattata tataaaaaag agctatacat aaaggtatgc tagctctctt
    17221 ttaatatgat atttagctga cctggttttt gaccccacac tcgcctactg gagggttcgc
    17281 ttgggttcgc cgcactacta ctctctctcg attttattat cggtaggtct tacttctaag
    17341 ccgcaccatg atcattaacc caatttttag tgttaactta cgtggatccc tttgcctgcg
    17401 actggcttgg actttcaacc acagacctaa atatcactta ttcattttaa tattttaaac
    17461 taaaattgtc aatatataag attattccat aatataatta tcatttgctt ttgtaagtag
    17521 agtcaataca aaaaccttat tagcacctga taactttaaa agtttagaaa tttcgtttgt
    17581 tgttagacca gtagtaaata tatcatctat taaaagtata tttttattat ttattaattt
    17641 aacattttct ttaacaacaa atacattttt taattcttct tttcgttctt tcttattcaa
    17701 tttatatagc attcttgtat atttttttct acttatgcaa tctaataatg gaatatttac
    17761 tattttactt aaattaaatg ctattttttg tgcctgatta aaacctcttt tatttagcct
    17821 ttttttatga agtggaacaa ataatatata atcaaatttt atgttttcta aatataattt
    17881 ttctttcatt atttgagcta tatacttagc cataaaggtt ttttggttat acttcagccc
    17941 taacaccatc ttttttgtca catcattgta ttcaatacaa gatattgatt tatcaaaata
    18001 aaagtcttta ttaaaacaat aactacactc ttcaataaat tctttttcaa ttgaatgacg
    18061 tattattggt tttccacact tcatgcaacc atcttgtata aaattcattt ctttaaaaca
    18121 acttttacat attgaataag tatttgtttt ctttatggat ttatcacaaa ttatgcaact
    18181 tatattctca ggatatataa aatccaaaca tttattaatc atatttttaa aattcttaat
    18241 aaaattatca ttgcatattt tcatatttat tctatcaaca acccttcttg tttaaattta
    18301 tttagcttat atcctaaatt tgaatacctt tgattaactc tattattttt tatcatatat
    18361 tcaagatatt tcacatctcc aactaataca actaattttt ttgctcttgt tactgctgta
    18421 tataataaat tacggctaag taacatagga ggagcccacg ctattggcaa tactacaact
    18481 ggaaattcac ttccctggct tttatgtatt gttgtacaaa aactatggtc aatttcatct
    18541 aattcatcat ataggtatga tacaatttta gtctggtcaa ataatatata aatagttttc
    18601 ttatctttat ctatgtgata aacatatcct atatctccat tatagatacc ttctccactt
    18661 tctttttggt cttctgtttc ccattttttt gtataattat tttttacttg cattacctta
    18721 tcgccaactc taaataatct tttagaaaaa ctttcttcaa ctttaaattt ttcttttttg
    18781 ttaagatact tctgtaactc tatattgaga ttagtaactc ccaattcacc ttttctcatt
    18841 gaagataaaa cttgtatatc ttttaattta tcaactttat aaaatttagg taatctttca
    18901 ttaaccaatc ctattatttc atttaaaatt tcttcattag ttgattttct tataaaaaag
    18961 aaatctttac cttttgtatt tagataaagt ggttctccat tgtttatctt atgggcattt
    19021 acaattatca tactttcttg cgcttgtcta aatatttcat ttaatttaac tacatttata
    19081 atattagaat taatcatatc cttaagtaca tttccagcac ctacagaagg taattggtca
    19141 ctatctccaa ctaaaattac tcttgttcct aactttatag ctctaagtaa attgtacatt
    19201 aagataatgt ctaccattga tacctcatca acaattatta catctgaatt tattgggtct
    19261 tcttcatcct taaaaaaagt taaatcatca tctgttgaaa atcccatttc tagcaaccta
    19321 tgtatagttt ttgcttcttt attagaagtt tcactcattc tctttgctgc tcttcccgtt
    19381 ggagctgcca aagtcacact tttaccatta ttttcaaata tatctatgat agcattaatg
    19441 gtagttgttt taccagttcc tggtccacca gttataatta atactccact attaacagat
    19501 tctttgactg ctaaaatttg tttttctgct aacttaattt tcttatcttc ttctaaaaca
    19561 ttaatctcgt tatctatatc tatttttaag tcttcaaatt catactgtga taattttact
    19621 atttgactac atactccatt ttcagctaaa tagtatggca taaggtatat aagattttca
    19681 ttgtttactt tctctatatg tattttttga ttataaacta gcatcattac acactctttt
    19741 attatttctc catttagctc caataatttt tccgaatctt gtattagaac atgctctggt
    19801 aaatatgtat gcccattact taaagattta tttaaagtgt ataagattcc ctgacaaatt
    19861 ctatctttag agtttttatc tataccaatt ttacttgcta tactatcagc tactttaaat
    19921 ccaattcctc ttatatcttc agcaagttgg tatgggtttt tatttataac ttctattgca
    19981 ctgcttttat attttttata tatttttagg cagtaatttg gtgttattcc aaaaggagat
    20041 agctgtatta ttatatttct caattctctg tcttcttcat aactttttac tatttgtttt
    20101 acttttttac tccctatgcc ttctacttct tgtagttttt ctggtgaatt ttgtatcacc
    20161 tctaacgtat ctacaccaaa cttatcaatt attctttttg ccattttttc accaatacca
    20221 tgtatcattc cagaagataa gtaaacataa attccttcta aggatgatgg tgttactggc
    20281 ataaaactat tcacttcaaa ttgagtccca taaatcttat gatttaccca ctttccttca
    20341 acttctatgc tttctccaat tgccaaagta ggcatacatc ctacaaccac aatttcatca
    20401 ttttcattta ccaggtgagc tattgtataa ccattatctt cgtttttaaa gactatttca
    20461 cttatcattc cttctaattt ttccattttt atctcctaaa aatactaata atcgctccat
    20521 ttaaatgtta gtataatttt atatattaaa actatgataa tcgtagaaaa gttttatatt
    20581 tttatactta acgtaatttc caactattat acattttatc agaaacaaaa aaaatctgca
    20641 tccataaaat atggtaccat gaaatacagg taccatattt aaatagctat tttattttaa
    20701 ttactctata tttagagtat aaattaaatt tcataagtat attacagtac taacttattt
    20761 cagatttatt ctccttgtac ccctcagttt tagtaattct tctataaagc atacatagta
    20821 aaattaaaat tcccatgtaa cctgtatatg gatatagtgt acctactaac ttatcaaatg
    20881 gcagtaaacc tcctatacaa gctaatatac ttaccactat tgttataatt ttaaacttag
    20941 ggtggtcatc ttctacaaat cggtttgtta ctgaccataa caatggtaca gctgtagtgt
    21001 atattcctag taaaagtact actgagaata aaatacctat tattggagat atcttatcag
    21061 ctaagtaaag tgctggtatc tcttttgtgt atatatttcc tatgtctgaa agtaatgcca
    21121 aattcatcat tatagctgct gccataagtg ctactccacc tactatacca ccccatacag
    21181 catctttctt atttactgca gaagctccca ttcctgtcaa gaatgttatt actattatta
    21241 cattgtaaca agtataaagt actcccgaga aatatccatt tgatgtagca gttttaatat
    21301 ttaaactatc aaccatattt ccagcatttg ataaagcatc aatattgcta aataaactaa
    21361 ttgctcctac taaaagtgta aatattatta ttataggtcc tatattacct agtattttag
    21421 aaagccttgt caaacctaat gaaacagtta ttagggaaac gattgccata ccaatcctac
    21481 ctacatatgg atttaatcca tagtactctg ataatgtagc tcctgctcct gcaatcatta
    21541 ttactaatgt tccaaataaa aacaatggac caaaccattc aaaaaatgtt ccaagatatt
    21601 ttccacaata tacttgataa atttttattg gctccttaag tttcaattct cttcccttat
    21661 ctataacttc tgctccaaac catgaaaata gtaccataga gattattcca cctataatac
    21721 ccgcatatcc ataaaacgta aaaaattgca gtatttcttg accagttgca aatccagaac
    21781 ctattacagt agcaacataa gctccagcaa aacttataac cgttttaata tttactttgc
    21841 ctgacataca ctactccccc ccaattaaaa ttatttttaa agtgtaagtt tattaagatt
    21901 atattagact cattattgtg atatttcaat atatttttaa ttatttaaca ttagttaaca
    21961 cattcttaac catcctttaa catatatccc ataatatgta taaatttagt agagtattat
    22021 ataaaatgta ttgaccagat taaatactgt ttattcatta tatgatgata aattaacatt
    22081 tattaaaaat atttaactca taataaaaaa gctgttagaa agtaatcata aaattacatt
    22141 ctaacagctt tttagttaaa aattattcac ctaaagcatc ttttcttaac tgctcaacct
    22201 tgtcagttct ttcccaaggt aaatctatat cagttctacc aaagtgacca taagcagcaa
    22261 cctttttata aagaggcttt cttaaatcta agtctcttat tatcgctccc ggtcttaaat
    22321 caaagtgttt atttactaat tcaacaagtt tttcttctga aaccttgcct gtcccaaaag
    22381 tatctataaa tatagataat ggtctagcta tacctatagc atatgcaagt tctatctcac
    22441 atttgtctgc aagaccagcc gcaacaatat tttttgcaac atatctagct gcataagcag
    22501 cagatctatc aacttttgta gggtcttttc ctgagaaagc tcctccacca tgtctagaat
    22561 atccaccata agtatctatt attatttttc ttcctgtaag acctgtatct ccttgtggtc
    22621 ctcctataac gaatcttcct gttggattta tataaatttt agtctcttca tcaagtaatt
    22681 ctgctggtat aacttcctta ataacatgat taattaaatc ttctcttatt ttatcatttg
    22741 atactgtttc acaatgttga gctgatataa gaactgtatg tactcttaca gctttacttc
    22801 cttcatattc aacagtaact tgagtttttc catctggtct taaataatca actaaacctg
    22861 tttttctaac ttctgtaagt cttcttgata atttatgtgc caaagatatc ggaagtggca
    22921 ttaattctgg agtctcatta catgcaaatc caaacattat accttggtct ccagctccaa
    22981 ctttttctat ttcttcttct atctcttctc cagtcttact ttctaggcct tcatcaactc
    23041 ccatagctat atctccagat tgttcatcaa tagaagttat aactgcgcaa gtatcacaat
    23101 caaatccata ttttgctctt gtatatccaa tttctcttac tgtttctctt actaatttag
    23161 gaatgtctac ataagcagat gtacttattt ctcctgctac taaaactaaa ccagtagtaa
    23221 ctgtagtttc acaagctact ctagattgtg ggtctttttc taataatgca tctaatattg
    23281 aatctgatat ttggtcacat atcttatcag gatgtccttc tgtaactgat tctgacgtaa
    23341 ataaatgtct tgccattttt attccccctt atttattctt aatttttatt ataggtttta
    23401 ccttattttt aattttagaa atttactcaa aataaaaaaa cctctccttt ataagaagag
    23461 gttaataatc gtaattacat actaattaac tgacctcatc tttcaagact tttgtcttgt
    23521 aggaattagc accttttccc atttgggtgg ttgctgggct tcacagggcc tatccctcag
    23581 cctctcttga taaggtaatc aagatttaag ttttgtttta ctttactagc ttactatttt
    23641 tttaaaattt tgtcaatatt tttagtcata ttttttctat tccttttttt gtaagacaaa
    23701 taaaattttc tatctatctg aaatttaaag ttaggtcttt ttaatgcttt ttattcatat
    23761 atttttttga agggggtgtt cctattgtat ttagctaaag taaactacag cgatgatgat
    23821 gttatacaga tgctaagttc agttctaaaa actataatta atgaaaatac gattgttgtt
    23881 tgcataggaa cagacagggc tataggtgat actttaggcc ctttagttgg aacaatactt
    23941 aagaacagta actttaaata ccccgtttac ggtacactag ataaccctat tcacgcctta
    24001 aatatatatg agtctttaga tactataaaa aatacacata tacaaggtaa cttcttggct
    24061 atagatgctt gcttggggtc gcagagtaac ataggtaata tacagattag agaaggacct
    24121 atcctgcccg gaaaaggtgt tggtaaaaaa ctacctcaaa taggaaatta ttctatagta
    24181 ggaattgtag ataaaattga tgaaaataat aaactttcat ttaataatat acgccttagc
    24241 tttatattag atttagctga aacaatagcc ttggctttat tagtatccac ctaatctaaa
    24301 tataaactaa atgataaaat tctttatata aaaaggtaga tattttagag tatctgatta
    24361 aatactccaa attatctacc tttttacttt tatatttcaa gtttttattt ttctactaaa
    24421 gcaaacatta attcaccttt acagacaagt tcttcaccta catatgcctt agcatctgcc
    24481 ttagctatat tttttctaag agttgtcatt tcaacttcca tagttaatgt atctcctggt
    24541 cttacttctc tcttgattct aactttatct atacctgcaa atacacctag ttttcctttg
    24601 ttttcctcta cagacatcat tgccactcca catacttgtg ctagagcttc tactattagt
    24661 actcctggca tcaaaggata ttctggaaag tgaccttgga aaaatggttc attaactgtt
    24721 acattcttta taccaacagc tcttttccca acttctagct ctgtaatctt gtctactaat
    24781 aaaaatggat atctatgtgg tattaatttt tttatttcat ctatatttaa cattctaaac
    24841 tctcctaatt attttctttt gaaagaccca ccagtttcaa gtaagtctat tgaacctaaa
    24901 gctattcctg taccttttgc aacacaagat aatggttcat ctgctattgt aacagttatt
    24961 cctgtcctct gttctattat tttatctaat ccatataata atgaacctcc tccagtcatt
    25021 attataccaa catctcctat atctgctgct aactcaggag gcgttttttc caaaacagca
    25081 tgagtagcaa ctactatttg ctcaacacat tctctaagtg cttccaacat ttctgttgaa
    25141 tttatagtta tagaactagg aagtcctgta attaaatttc ttcctgttat cttcatatat
    25201 ttttcttctt ctctcttaaa tgcagaacct atttcaaact tgattttctc tgctgttctt
    25261 tctcctataa gaagattgtg ttgttttttc atatatttaa ctatagcatc atcaaatcta
    25321 tctccaccaa cctttataga ttcacttaca acagctcctc ctagagatat tacagctata
    25381 tctacagtac ctccaccaat atctataatc atattaccat taggctgtga tatgtctaca
    25441 ccagctccta tggcagctgc tattggttct tctatgatat atacttctct agctccagct
    25501 tgtctcgtag catcttctac tgctcttttt tccacttctg tgacacctgt tggtacacat
    25561 accataattc ttggcataaa aaatcttcca aatccctttt tatcaactat cttatctata
    25621 aaagatttta acatcttctc agttacatca taatctgaaa ttacaccatc tcttaaaggt
    25681 cttatagcga ctatattacc tggtgttctc cctatcatct ttttagcttc ttctcccaca
    25741 gctaaaacag aatctgttct tttgtctatc gccacaacag atggttcttc taaaacgatt
    25801 cccttaccac taacataaac aagcacattt gcagtaccta aatctatgcc tatatcagct
    25861 ccagccattt tttcaactcc tttatttata atattagatt atattataag cctattttaa
    25921 tactcctata ataaagcata tacaaaaatc tcgctttaat caagagaaaa taaatctttt
    25981 gagagtatta ttattttgct atttataatt acttaatata tttcttttta taatttaatt
    26041 caataaaaaa agtaggtttt taacctacat ttttttatgt tccttctcat attttaattt
    26101 cgttgctaat ccccctctaa tatgtctctc tgatttattt ttgtccagca catttttaac
    26161 ttctttggca agggatggat ttatttcctt caatctttct gtaacatcct tatgaattgt
    26221 acttttactt actccaaatg tcttggcggt ttgtcttact gtagtgtttt tttcaagtat
    26281 atattttgct accactatgg ccctttcctc tatatgagat ctcaaagatt acccccctca
    26341 cataatagct ttaatgatat atatgtaaaa acaccaaaaa atataacaag cttaaccaaa
    26401 tatgattaaa cttgttatac tattacttaa ttagactcat tgggtctata gatttttctc
    26461 cactatacgc ctctaaatgc acatgtatac cttcttcact ttctatttga gaagtactac
    26521 ctacagtacc tatagattgt ccttctgtta ccttctgttc tttcttaaca ctaacatttt
    26581 tatctagatt tgaatatact actacaatgt tattgtcact ttttattttg actgattgtc
    26641 catattcttc atcatcaaat acatctacta ctattccgtt taatagagat tttacttctt
    26701 tgcctttagt acaactaata tctacacctt tatgagtttc ccaaacatca agtgttttag
    26761 aataactagg ctctttttct gaatatcctc ttattacttt atctccaatg taatttaatt
    26821 ttgttgtact cgatttattt tcctcttttg acttagcttt ttctaagttt tgttttgaat
    26881 ccgttgcagt agggatagca tctttcttgt ctttttcaat taaatgtatt tcctcttcac
    26941 tatctttatt agcattttcc ataattcctt tattagaagc caatttatct acattattat
    27001 ttgtaaacca aactccacct actgctaata aacaaacaca tacaaacaaa gataaataaa
    27061 aaccatcttt ttctaacagc tttttcttca ttttgcacct ccaaatatat ttctacattt
    27121 ggattattga cgtgtttttg gcattttata caattattag tatatatctt ttatttttgt
    27181 atctgtgtag taatgtgata atatatcata atatttatag ccctcttctg ccattccttc
    27241 tgctccccac tggctcatac caacaccatg accatatcct tttaccacga aatctatgta
    27301 tccttctcca aattttatgt caaagtttgc agagttcaat ttaaaaacag ttctaatgtc
    27361 ttttccagtc aattctttat ttcctaattt tattttttct acagttccgg catcacttct
    27421 tttagttatt gaaacttgtt tacttaaatt attcacatct atcacaatag tgctatatgc
    27481 ccttctaaga cttttaacaa aatctgtgtt acttatttta agtgttgacg caaatttagg
    27541 ggaatactta tcataaggac tttccacaga ctttaaataa ggatattttg cagaaaatac
    27601 ttcttcacta ttttctgttt ttcctgaaga tgtagaaaaa taaagaggaa gtatcgcctt
    27661 atcattataa gttattattt gacctttagt tcctctaact gcttcttgga ttttggaata
    27721 tttattctta atccattctt caccatttaa ttttttaaga gtatcataac tcttatattc
    27781 ttgacaatgt ttataatcag tacatactac tgcattttta tgtttactag atttaccatg
    27841 ttcctgttta tatactacat atgttctagc tgctacagat tgagctttta aagcttctat
    27901 atcaaactct gaagacatct caccagctag tactccacat aaatagttct ccatgtccat
    27961 cttttcggtt ttccctgtta tatgattata tacattgata attggtgctt ttttatttac
    28021 agtttcatag ttcactgtat ttttttcttc tttattacct ttgtcttcaa tatcactttt
    28081 tttaatagtt ttgtttatag atactgggct ctctggtttt tcagttaatt ctacattttt
    28141 ataagaaact aaagttataa gtgatggaac taacacagag caagtcacaa atcctaacaa
    28201 aacaaccaat gggttcttca tatactctcc tccttagctt ttaaaacctt gtatttaagt
    28261 atatgaacaa ccctattaat ttataacttt cttctataaa ccaattatta atcttctact
    28321 atttcgattt gaccacctag tgctgttatc ttcttatcta tatcaacata acctctttgg
    28381 atatggtata tttctcctat ttgagtttct ccttctgcta taagaccgca taaaataagg
    28441 gctgcccctg ctcttaaatc agtagcgttt actgctgcac cgtgtagctc atctacacca
    28501 ttaacaaccg cacttcttcc atctattttt atattagcac ccattctatt aaattcagca
    28561 acatgcataa atctgttttc aaaaacagtt tctataacta caccagttcc attggctaca
    28621 gtaagcatag ccataaattg tgcttgaaca tctgtaggaa atcctggatg tggtaaagtt
    28681 tttatatcta ttggttttaa tacttttgga cctacaactc ttacagaatt gtccatttct
    28741 gttatttcac aaccagcttc tcttaattta gctacaactg gttttaaatg ctccattaaa
    28801 acattttcaa cagttatatc accttttgtc atagctgctg caaccatata agttgctgct
    28861 tctattctgt ctggaattac attatgttct gctcctttta actctttaac accttttatt
    28921 tttatagtat tagtacctgc acctttaaca tctgctccca tttcgttcaa gaagtttgct
    28981 aaatctacta tttctggctc ttctgctgca ttctctatta ttgtagtccc ttctgctaaa
    29041 gaagctgcca tcattatgtt ttcagttgct ccaacagatg ggaaatctaa atataattta
    29101 tttcctacta acttttctgt tgctgcttca acaaaaccat ggtccatttc tatctttgca
    29161 cctaaagctt taaatccttt taaatgtaaa tctataggtc ttgttcctat agcacatcct
    29221 cctggcatag aaattttcgt actattaaat ctagcaagca atggtcccat aaccaaaaaa
    29281 gaagctctca tctttcttac aagctcatat ggagcttcac aagtttttat attgcttgca
    29341 tcaacagtaa gtgtattttc tttatattca acttctgctc ctacatgtct taataaatct
    29401 gatataacat gaacatctct taaatttggt actccattta atgttgattt tccgtttgct
    29461 aataaagtcg ctgctattat tggtaaaact gcattttttg caccatctat tttaacacta
    29521 cctttaagtg gattactctt ttttactatt atcttagcca tattttttga tctcctcata
    29581 attaataatc taattttata attggtgtgg caatatatat gagtgttttt ccgtcatcct
    29641 cattatattt aattcctata tttaaattaa ttttttcccc taaatattct aattcataat
    29701 catttaatac tttactatat gctgttatag ataaaaagtt tacgtcttta acttcatcta
    29761 tttgcttagc actcatatta tataatattt tttgtaaaat atcatcatat ttgttgtttt
    29821 gtaacctttt tgtgtattct ccaacaatac aagcagacac atctacttga gatgaatatt
    29881 tatttaacac attttctacg ttactataaa taacctcttt atgtttatat actttgttat
    29941 ccaatatgtc aactactatg taactttctt tgttattttt atttattcct tttatagaaa
    30001 tttctttatc gctacctttt atagtagcac ataattgtat tttatcgcca gacccttttt
    30061 gattccaatt tattcctttt ttatccaact ttagattgtt aattatttct ataaaaatat
    30121 tatgaaattc tttatt
//
LOCUS       DAELJO010000023        29972 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELJO010000023 DAELJO010000000
VERSION     DAELJO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.023"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(67..2037)
                     /gene="ftsH"
                     /locus_tag="KPB88_000956"
     CDS             complement(67..2037)
                     /gene="ftsH"
                     /locus_tag="KPB88_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0053254.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2071..3459)
                     /gene="tilS"
                     /locus_tag="KPB88_000957"
     CDS             complement(2071..3459)
                     /gene="tilS"
                     /locus_tag="KPB88_000957"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG0053255.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3642..4070)
                     /locus_tag="KPB88_000958"
     CDS             complement(3642..4070)
                     /locus_tag="KPB88_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG0053256.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4087..4893)
                     /locus_tag="KPB88_000959"
     CDS             complement(4087..4893)
                     /locus_tag="KPB88_000959"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG0053257.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5095..5580)
                     /locus_tag="KPB88_000960"
     CDS             complement(5095..5580)
                     /locus_tag="KPB88_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0053258.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5631..6290)
                     /gene="spoIIR"
                     /locus_tag="KPB88_000961"
     CDS             complement(5631..6290)
                     /gene="spoIIR"
                     /locus_tag="KPB88_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG0053259.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6435..7115)
                     /locus_tag="KPB88_000962"
     CDS             complement(6435..7115)
                     /locus_tag="KPB88_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0053260.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7099..7989)
                     /locus_tag="KPB88_000963"
     CDS             complement(7099..7989)
                     /locus_tag="KPB88_000963"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG0053261.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8107..9657)
                     /locus_tag="KPB88_000964"
     CDS             complement(8107..9657)
                     /locus_tag="KPB88_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0053262.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9751..10017)
                     /locus_tag="KPB88_000965"
     CDS             complement(9751..10017)
                     /locus_tag="KPB88_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG0053263.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10165..11025)
                     /gene="yabG"
                     /locus_tag="KPB88_000966"
     CDS             complement(10165..11025)
                     /gene="yabG"
                     /locus_tag="KPB88_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG0053264.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11109..12047)
                     /locus_tag="KPB88_000967"
     CDS             complement(11109..12047)
                     /locus_tag="KPB88_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG0053265.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12157..12318)
                     /locus_tag="KPB88_000968"
     CDS             complement(12157..12318)
                     /locus_tag="KPB88_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053266.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12328..12825)
                     /locus_tag="KPB88_000969"
     CDS             complement(12328..12825)
                     /locus_tag="KPB88_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0053267.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12940..13650)
                     /locus_tag="KPB88_000970"
     CDS             complement(12940..13650)
                     /locus_tag="KPB88_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG0053268.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13826..14356)
                     /locus_tag="KPB88_000971"
     CDS             complement(13826..14356)
                     /locus_tag="KPB88_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053269.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14373..14603)
                     /locus_tag="KPB88_000972"
     CDS             complement(14373..14603)
                     /locus_tag="KPB88_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053270.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14618..16012)
                     /locus_tag="KPB88_000973"
     CDS             complement(14618..16012)
                     /locus_tag="KPB88_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0053271.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16138..17391)
                     /locus_tag="KPB88_000974"
     CDS             complement(16138..17391)
                     /locus_tag="KPB88_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0053272.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17442..18596)
                     /locus_tag="KPB88_000975"
     CDS             complement(17442..18596)
                     /locus_tag="KPB88_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0053273.1"
                     /translation="MSNNINQNLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19156..19725)
                     /locus_tag="KPB88_000976"
     CDS             complement(19156..19725)
                     /locus_tag="KPB88_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0053274.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19928..20155
                     /locus_tag="KPB88_000977"
     CDS             19928..20155
                     /locus_tag="KPB88_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0053275.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20374..21117)
                     /locus_tag="KPB88_000978"
     CDS             complement(20374..21117)
                     /locus_tag="KPB88_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0053276.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAAPQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21285..22157
                     /locus_tag="KPB88_000979"
     CDS             21285..22157
                     /locus_tag="KPB88_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0053277.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22157..22822
                     /locus_tag="KPB88_000980"
     CDS             22157..22822
                     /locus_tag="KPB88_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0053278.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22843..23700
                     /locus_tag="KPB88_000981"
     CDS             22843..23700
                     /locus_tag="KPB88_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0053279.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23856..26402)
                     /locus_tag="KPB88_000982"
     CDS             complement(23856..26402)
                     /locus_tag="KPB88_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0053280.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAISIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26402..27088)
                     /locus_tag="KPB88_000983"
     CDS             complement(26402..27088)
                     /locus_tag="KPB88_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053281.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27171..28160)
                     /locus_tag="KPB88_000984"
     CDS             complement(27171..28160)
                     /locus_tag="KPB88_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053282.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28147..28833)
                     /locus_tag="KPB88_000985"
     CDS             complement(28147..28833)
                     /locus_tag="KPB88_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053283.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29333..>29972)
                     /locus_tag="KPB88_000986"
     CDS             complement(29333..>29972)
                     /locus_tag="KPB88_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0053284.1"
                     /translation="DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVE
                     VSLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIA
                     KDMKELGYTFVATEGTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTK
                     GNDSTRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9723 a         5451 c         3443 g        11355 t
ORIGIN      
        1 tatactttca gttttagtat ttaatccaca tcagataata atcttaaaat gaccaaggtc
       61 ttaaaactat ttatctttat cagatttatc ttctaaattt ttatttatat caataacatt
      121 gtttctttct tcttcatctt tttctaattt tatagttaat tgttcttcaa cttctttaga
      181 agaattttcc tctgtaactg cattttcaat ttcattcaga ggtaactctt tattaaatgc
      241 ttttacaaat tgttctgcat ctaaagtttc atatataagt aaagcttgtg ctacatactc
      301 taatctatcc atattttctt gtaatatact tcttgtctta tcatatgcac catcaactat
      361 acgctttgtt tcttggtcta tctcaaaagc aacttcttca gaataatttc tcttacttga
      421 aaaactatta cctaggaata cttcatcatc actgtcaaat gacattggac caagtttaga
      481 actcattcca tactttgtta ccatacttct tgcagtagct gtaactcttt ctaagtcatt
      541 tgaagcacct gtagatacat ccttaagtac tagctcttct gctactctac caccaagtaa
      601 aactacaata ttttctttca tttcattttt tgtagcatag aacttatctt caacaggaag
      661 ttgcattgta aaccctccag ctcttcctct tggaactatt gtaacttgat gaacaggact
      721 tacttcttca agtacatggg cacaaactgc atgccctcct tcatggtacg ctgtaagtct
      781 tctttctttc tcacttatta ctttcgattt tttagccaca cctgctataa cctttgttat
      841 agcttcttct atagtttcca tcttaatttt cttttctctt tttctagctg ttaatattgc
      901 agcctcattc attaagttct ctatatcagc aggagtaaat cctggtgttc ttctagccaa
      961 gacatccatt tttacatcat cacttaatgg tttatttctt gagtgaactt taaatatagc
     1021 ctctctacct tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct
     1081 aagtaaagct gggtcaagta tatctggtct atttgtagct gccatgatta ttataccttg
     1141 atttacccca aatccatcca tttcaactag aagttgatta agagtttgct ctctttcatc
     1201 atgacctcca ccaagacctg cacctctttt tctaccaact gcatcaattt catctatgaa
     1261 tatgatagct ggagcacttt tcttagcctg ttcaaataaa tctcttactc ttgaagcacc
     1321 aaccccaaca aacatctcaa cgaaatcaga accacttata ctgaaaaagg gaactcctgc
     1381 ttctcctgca actgctcttg atagatatgt tttaccagtt ccaggaggtc ctaccataag
     1441 cattcctttt ggtattcttg cacccaattc aatatatttc ttaggattct ttaagaaatc
     1501 tactacctcc tgcaaatctt ctttttcctc atctagtcca gctacatctt taaatgtaac
     1561 tctagttttt tcatcatctt tatgaacctt agcttttgat ttgccaaaac tcataacctt
     1621 tccgcctcca ccttgagact gattcataaa cacgaaccaa ataatcacca tgaagaatat
     1681 taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag cttcaccacc
     1741 aaaagttagt ttcccagcct tagcttggtc taacacctca tctgcaagtt tatttcccat
     1801 cacttctgtt ggaacatatg acttaaactt tgtatttgta tcttttatag taccttctac
     1861 agaagtttga tttacaaagt ataatcttga tatattctca tctgttaatt ctctatatac
     1921 ttcagagaat tttaaatctt ttattttttc tgtaggctta cctgagaatt gaactatacc
     1981 tactataatt ataaaaacaa gtagataaaa acctgcccct tttagcaact tattcaatat
     2041 agaagccctc ctttcataca ttgctttgcc ctataatttg ttaaaattaa tttttaatac
     2101 ttcttttgtg ttttcatcaa ttttataatt ttcactgatt ttataatctc caacacatat
     2161 tattccttca ctatctgtga ttataggaat tttacatctt tcttctcttg ggatttttaa
     2221 atctatgaat aaatctttca cttttttact tcccatagca agctttatct tatcaccttg
     2281 ccttctgctt cttattacta tacccccttt taccttatta aaatcaaacc ctttagatga
     2341 attgtctaat ttcatactct tatacctatc tatgctcatt acttgggtct caataatttg
     2401 ttttaattct tttattttaa taaacccatt actaggtaca ttgtaataaa attcaatttc
     2461 ctcattaaca atttcctcat tcgttaatat tatactgtct ttttttctat aaacaaatat
     2521 atttcttgga agagttaaca ttttatttac ttttgaatta tcttctaaag acattatatc
     2581 ctctatatgt ctttggtcaa caaaattagt atctcctagt atatgtttta tggaatttct
     2641 aagaactcta actttgatag catcatgtaa gcaaacaaaa tcgtctaaat ttatctctac
     2701 aaagcctttt tcttttatat tcgaaacttc tctaaattta gcttctgcct ctttctcaat
     2761 ataatcgtta tcacttttta aactattgct catcctcact atagattcta ttacatttga
     2821 attaaaatta tccttcatat atggtaaaag gtctaacctt atcttatttc tagtgtatat
     2881 attttctaaa tttgttttat ctatcctcgg atttaaatta taagcttcac aatactcctc
     2941 tatctcattt ctttccacgt ctaatatggg tctaattatg caattatctc taatataatc
     3001 aattcccttt aaacctttta aacctgttcc tctcatgata cgcatcataa ctgtttcggc
     3061 ttggtcattg agattatgac ctattgctat tttattggct ttaatcttat ccttaatttc
     3121 ataaaacatt tcatatctta gcttccttgc tccttcttct aatgataatc cctcattttc
     3181 acaatatttt ggtacattaa tagactttac aaagaatata attcccatat cctcacaaag
     3241 tttagataca tataatgcat ctttttgagc ttctatccct cttatttgat ggtttaaatg
     3301 tgctgcatat atttctatat taaaatcttt ttttaatcta tttagtacat gcaataaaca
     3361 aactgaatca ggtcctccag aaagacctaa aactatttta tcgccttttt gtattaaatt
     3421 atgtttattt atagtactta gtaccttatc aaatatcata tttatccctc ttacacttag
     3481 ttttattcac ttttaagtat actttctata aaaaaacgct aaacccttgt atcaatttta
     3541 agaaattaaa gtttaatata aatataataa taccaaataa attaaataaa aggtagtttt
     3601 ttcactacct ttataaaatt tctggaaaat agccatagtt attatttagt acctatgtat
     3661 atatctattt tattcctacc taaaaataaa tcttgtacct ttatattata gtctattttc
     3721 aattttatgt gttccccttc ttgtatattt atatctaatt ctcttgtatc aatatcaatt
     3781 aataaagtac cttgtggggt cacatacttt gtagtactac tttctccaca tttaaacagc
     3841 attgttgagt taatagcacc aaactttttg atactaactt gatttttaag tatctttata
     3901 gtattagtta tttctatatt ttcttctttt tccttgtaaa taacatatat tccatcattt
     3961 ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc tttttcatca
     4021 tattgtctag tatttatact tatttttaca ctcaacgatt tttcctccat agattcttca
     4081 tataaactag tatttttgta tttctacttt ttgaatctta tcaatttttt tctttaagaa
     4141 ctctcttgct atatctgaaa atttttcagg tatatctgat acataatatt gataagttcc
     4201 atgaatttct gtattgttta taatattttg cacctctaaa atctttttta aatcctttgc
     4261 agtctcttta gctggattaa caagttttat atgctctcca acttcttcac ctattgttct
     4321 ctttaaaata ggataatgag tacatcctaa cactaaagaa tctatatttt tttctttcaa
     4381 ttcttgtaaa tatatctttg ctgtcaattt agccacttct gtatttgccc atccttcttc
     4441 aacaataggt acaaataaag gacatgcttt attaacaatt tctatactct catctatttt
     4501 agatatttcc aagttgtatg cttttgaact tatagttcct tcagttccta ttattcctac
     4561 aattttgttt tttgtagaac ttacagctgt tctagctcct gcttctataa ctccaattat
     4621 cggtatatca tatttttctt gagcttcttt taagcttctt gcagtagcag tgttacatgc
     4681 tataactata gcttttacac cttttgtttt taaaaagttt atagcttgaa aagtatattt
     4741 aattatagtt tctttggatc ttgaaccata tggtattctg gcagtatctc caaagtatat
     4801 tatatcctca tttggtaata tcttcattat ctcttttaga actgttaatc cacctaatcc
     4861 cgaatcaaaa actcctattg gtttattgtt catctttata caacacctac tttacatttt
     4921 tgtgtttaac aacaacttat ttatcgctaa aatttatatt tatattcata attaatatat
     4981 tattacctaa atgattatta tagtttattt tacacaaaaa ataaaggagc tatcttaaat
     5041 tagaaaataa tttctacaaa aagacaactc ctttcactac ttaaaaattt aaatttattt
     5101 tgcaaacaca tgatttccta ttctcatata aggattcaat ctatttatcc atttgcaagt
     5161 ggctatttct ggattccaaa aatatactgc tttattagtt gggtcagttc cataaagagc
     5221 ttcctgagct gctttacgtg ctgagtctgt tggtgccatc tttattgaac catcaagcac
     5281 aactgagaaa gcattttttt gatatatgac ttcttctatt gtgtctggaa atctagggtc
     5341 caatactcta tttattacta cagctgctac agcaacctgt ccctcatagc tttctcctct
     5401 agcttctcct gcaaccaact tagataataa catcaaatca ttatcagata aatttattac
     5461 ttctttcttt gctatatttt tttcttgttt gtttactttc ttaatatttt ctgttggttc
     5521 atatgcaaat acctctttaa taggcataaa tataaaagtc aaagctaata gtgtagccat
     5581 cagtttactc cacctgtcca tgattaaaac cactccttac attcttattt ttaaaatttt
     5641 ttctctaaat ccttgactac ttcaagtact ttaaacttaa tttttactct acttgttttt
     5701 tttggtgtat cttgttttat taacttatat tctttatcag ttaaaacttc cttcaactta
     5761 tcatctgtag atttatctat cactccatta gattcatcaa caaaacatag tggagggaac
     5821 ataacacacc accagttttt tccttcacct ttcccaatga taatctttaa tgctttatat
     5881 tcaccagctg gaagaactat attagaatat tgtttagtag ggaaattgct atattgtata
     5941 cctaccttta cactgtaatt atatccattt ttaagaataa tatttttact tatttcttcc
     6001 agactactat attccttttt tataatagct tcactttctt ttatactttt tgaattttga
     6061 agctttggct gtaaatattt tattacttca tctctaactt ttagtttaag ttcttggtct
     6121 tcatctgtat tgctgtttgc tataacatga aatcttatta atttatcttt ataatctttt
     6181 gatatattgt ctactttttt tacctcacca tttattacta ttgtcattat tgatatgact
     6241 gatataagac ttaatattaa tagtcccaat ctaatcttaa tttttctcat tgaaaattcc
     6301 ccctcaagat aaatttatat catcaattat gtccaattaa atttaattta ttcataatat
     6361 tttttaatac taataaaaaa agctatctta aaatgattac aatcatttta agatagcttt
     6421 ttaaacctta tattttataa gtctttacta atcatagcct tatctatcat aaactgctct
     6481 gctttttcta tatgctctgt agcatacttc ttggatagct cactatcacc tttaattata
     6541 gcatcaagta aatatttatg ctcttcaaca atattatgtg atgtatcata agcagacata
     6601 taggtaactc taaatctata tacttgttcc cacaatgagt ttattaattg gattaatttt
     6661 ttattattag ttgctttaaa tatacattca tgaaattcta catcaagttt taataacgca
     6721 tctacatcgg attccaaagt gcttttttta aattcttctg atatatcttt aagcttttta
     6781 atatcttcat cactaattct ttcagcggca agatatgctg ctaagccttc taggcttgct
     6841 cttacttcta atacatccat aatatccttt aatgacatat ttgccacata cgcacctttt
     6901 ctaggcaaca taacgactaa accttctaat tcaagctttc ttattgcctc tctcactgga
     6961 gtcctactta ctcctaattg ttctgctagt tgcacttcca ttagcctttg accaggcttt
     7021 aattttcctt ctaagattgc ttctcttaaa ttctcaaaga ctacatctct taatggcttg
     7081 taattatcca agttcagttt agttaaattc tccacaaatt tcaacccctt tttgactgct
     7141 attaacaaca tacacctgtt tatatttttt taataattct ttttttccaa ttagagcatc
     7201 ttctttattt ttaaatagtc caaaaactgt aggaccactt ccactcatca tagaacctaa
     7261 agcattattt ttcatcatta cttgttttat atcacttatt tctttgtgtt tacctatagt
     7321 aacattttca agaatattta ccatgctttc tgatacagct tttatatctt cagattttaa
     7381 acactctatc aaatatttat tatttggtct ttttttaata ttattcaaat ctaatccttg
     7441 ataaacttct ttagtagaaa cgaataagtc tggtttgcat atcaatatat ttgtatcaca
     7501 tggtaaacct ttgatgtttg ttagcttttc tccaatacct tgagctaagg caggtcttcc
     7561 tgatatacaa aaaggaacat ctgcgcccaa attaagtcct atttctttta attcatcttc
     7621 agataatcta agttcccata ggtgatttaa acctactaat actgcggctg cattagaact
     7681 tcctccagcc atccctgctg ctacaggaat atttttttct atgaatattt ctacaccttt
     7741 ttttatataa aatttatttt taagtatttt ggcagcttta taaacaatat tatcttcatc
     7801 taaaggaata tctaaactag tgctttttac ctttatttca tcttcgtcta attcttttat
     7861 ctttactata tcgtataaat ctatcgtttg cattatcatt tcaacaaaat gataaccgtc
     7921 ttgccttttt cccaagacat caatggataa atttattttt gctctactct ttagtcttat
     7981 aaaatccatt ctatcctcct tgtttaaaga ataatgcatc actaacaata atattatact
     8041 acaaaaaata aaataatagg gtattataat accctattat acttaattac tttaaatttt
     8101 ttaatattaa tctactaata cgactttttt ttctaaaata agacacttac caggcttaat
     8161 tggttcatca agttttatat cattaagttc tgcaatttca ttttctgttg tattgtattt
     8221 ttttgctata ttccagaaag tatctccttc tttgcatata tatacgatta tgctaggagc
     8281 tttagataaa tcatatacac cttggtcttc tcccttgata ataaaatttt ctgctttctt
     8341 atctaatgct tcagtaaatc tcttaatttt gataatcaag tctatctggt ccctattcaa
     8401 atcaacttcg actttatcaa tacatgctgt attaaacaca gatgctgtat cagttagatt
     8461 atccatttct atatcatgtt caaatggaat ttcctcacta attttataaa ccaattttaa
     8521 accttcaact gggacaaata aaatttcaac ttttatgatt ccttgtataa tgcttttatt
     8581 tccttcaata taactatttt ctatagacat ggttggacaa acgcttacaa tatcttttat
     8641 ttgtatatca tcattatcat ttcttatgcc ttctctaacc ataaatgttt cttcactacg
     8701 tcttaaagtc ttatttaatt gaataggttt gtggtcaaat tttattatct tttgaggcga
     8761 atatgcatct tgtaatactt ctcttgtaac ttcatctgta acttttacct tacaacaagc
     8821 catacaatct atttctaaaa gacctgtgtt gctttcacta ttttgcttga aaatatgatt
     8881 aaaatctgac atagacaaca atacttcttc agtcatacca tcactaactc cagggacttc
     8941 tacaaattga gtaaactcaa tacctactct atccaactct acaagttcac cctcatatgt
     9001 acaagctaac ggatttattt ctaatactcc accaataata actttattgt cagtcactct
     9061 actttctttt actttaacac atggatttaa acttattata gattgaattt cctctgtgtt
     9121 tattgttata gtatctctta tagaactttc tgctttttct attccaacta tatcttgaaa
     9181 acaaatttcc tttctgtgct tttgtatgcc ttccacttgc gccacatctt ttacaatgtc
     9241 taacctttgc ttttcaaaca aacttcccct tatattcata agagctccta ctttaatctt
     9301 tctctcattc attatagtac aatccatatg ctctacttct gaaaataaca tgtattccat
     9361 atcttgaata acattgtctt tttctattac ttcgtttata tctacttttc cgtctacatt
     9421 agaaatagta tttttatcat ctgcaatata gattacatta taattaaaac tacctctgca
     9481 aagtatcttc ccatctgcca tttcaatttt tttaagtgat atgtatcctt ctgtttttac
     9541 aatttcatat acatctaatt ttttgtctgg cacaactgcc tccgcttcaa tgaatgtttg
     9601 aaattttcca aagtctattc tattgtcaac tttaattaca tctttaatta attccatagt
     9661 ctacctcctt aagtattata aaaatatcac actatattta tatttaagga tcaaaaaaaa
     9721 aattacacct atttttaaat aggtataatt ttaacttatt tgtaatttat cttgatctct
     9781 aaaaacttgc aactttacat tagatgttaa taaatctgag taactataag atactctagg
     9841 atatccatta ctttcatcat ctaactttat aacaaacacg cttggataaa ctttttctag
     9901 tataccctct tttgtaataa tctgctttct tcctttatta gctttcaaca atattttctt
     9961 tcctatatgt ctctctaagc ttactcttat cttatctaga gtttgaacag tagccacaat
    10021 atcaccttct ttaacgttta tatgtttaca taatatcaca tataaacatt tttgtcaaat
    10081 gtttaaacta attattttat atattttttg caaaaatgtc aatactattt ttgaagatat
    10141 acagtataat tatgttaatt tttgctaatg taatattgtt tttggcattg cccatctaaa
    10201 ttttcctctt gtttcagaac caccaattcc cttaacacct gtaatagtct gctctataac
    10261 ctcatcaata ggaactacta catcaattct tgaacaagca attttctcca ctataaaaac
    10321 agggtctaat gcatgtatca ttattcttcc aggcgaactt gcatagtttg ctcctgcact
    10381 tattattttt tcataatttg attggcatgc tcctgcgaat atgacaagat tatccaaatt
    10441 tggttgccat ttacgtgctt cttttactgt tttaacaaaa tttgaagagt ttctatagtt
    10501 actcatatct tctatatctc ctctttttat tgtcattgca tcatgacctg ttattactag
    10561 tatatccgga ttatattttt ctagtagtgc tcttacttct ttatactgat tttgctctga
    10621 aacagctaca cctactgcag gtattccaag ttgagcatat acatccaagc aaatttttaa
    10681 atattcttta tctccatcta tctgtagtac ttttccaggc attccatatg tgttaggatt
    10741 taattgaagt tttggaactg ctctagtcat tcttttttgt ctttctttag cttttcttac
    10801 tgacttatat aataaatttt ctacattttt atctattaat atatctttta tatcaggtgc
    10861 ttttactaat tctaggtcat caatataagc atctgctatt actctaaatg caattccctt
    10921 tagtatagct attttctcat tattttcatc tacaccaaaa gagactattt taaatactat
    10981 atctttgtta tgtgactttc tagctacaat gtctcctacc ttcatagtta aacctccaat
    11041 aaatttttat cttagtttat actatgttcg atataaaact ttgtttccta atgtcaaaaa
    11101 aacatttctt ataatacttc ttttatcact cttaaagcat ttttatagta tatcttttct
    11161 atatcatctt cactaaaccc ttcttttttt agaggttcat acagtttacc catctgagat
    11221 atatcttcaa tctcaacttt tgaatctatt ccatcaaaat ctgaccctag agatacaaca
    11281 tctattcctc caacatttac aatatgtttt atgtgtctaa ccatatcttc aagtttactt
    11341 tcacttttat cacttaaaaa taagtggaag aagtttatac ctgtaactcc acctttattt
    11401 gctaatactt taatcatatc atcagttaaa tttctagaat gattcatcat tgctcttgaa
    11461 ttagaatgtg tagcaattat tggtttagaa gaaatctttg caatttcata aaatccacca
    11521 tctgaaatat gagaaacatc aaccaacatt cccaattcat tcatcttatg taccacttct
    11581 cttccaaaat cagtaagccc tttttctcta tattctactt tattatgagg aaagctcaat
    11641 tcattcacat gattccaact tattgtcatc attctaaccc ctaagtcgta aaatttcttt
    11701 aaattttcta atttaccttc aagtactgca ccttcctcaa tagataaaag agctgtaagt
    11761 ttaccttctg attggttttt cataatttct tcatagttag tagctaaagc tatttcatca
    11821 ctattttttt tcatttcctc atgaaattta tttgccatac tcatgcaata atcaaaagga
    11881 tgttttactt cttcagtatc aacaaaaaga gcaaatgttt gagctaatga atcccccttt
    11941 ttcaatctgt ctatatcaac agaatattta ttagacttaa gttcacttgt ttctacattt
    12001 tccattaatt ttgctatagt atcacaatgt aaatcaagaa atttcatatt tataccccct
    12061 gtatatttta tatttatatc taaaaataga aatagttgtc cttatagaca actattgaca
    12121 actatttttt aatattcttt ttaatattac ttaatcttat tcttctttta agttaggtat
    12181 tactttttta acttcttcaa tttgatgcca tgttttatct atattatttt cactaatata
    12241 tttttttaaa tcagttaaag atgtaaaatc tttaactgat aaattgtcat catcatctgc
    12301 aaatcttaaa ataaacatga gactccctta ctttccacaa catttcttat atttttttcc
    12361 gcttccacat gggcatgaat catttcttcc tatttttgct tctttaacta ttgttttaga
    12421 cttcttataa gcctttatta tttcttttct ttggtctgct ggtagtatat cttcccaacc
    12481 ttgtaaatta tatagccaat gagcctcaac agttaccata ttgttatata atttttccca
    12541 atctattttt atagaaactt ctttatcagc ttctacttct tctaaaacta tatcttcttt
    12601 taagctttca cttattccat ctatgaatcc catgaaaaat tcattagtag ttccaaattt
    12661 ttcagccaat tcacttactt taccttcaac aacatccatt ttattattta atatttcatt
    12721 gtatattcca gcttcaactt tcaaatattc ttcccagaat tttacttctg cttcttgact
    12781 ttcatgatta tcacttaaat ttctccattc agtatataaa ctcatacttt ttcctcctaa
    12841 gtatacctat atttttatag tttttatagt ataaaatctt ttagttagat tggaagtttc
    12901 ttctttttga ttataccata ttctatttaa agttaactct tatattatat cataattaaa
    12961 caattctttt aatactttta tagcatactc ttggtttata aaataagggc ttgtcgcaat
    13021 tgttattact ttggcttttt taattaagtt ttttatcttg taaaatttga gttcaaatgg
    13081 tatatagtcc atatcacttg aaaatatatc caaatctata tcaagaacaa actctgattc
    13141 tacttctaaa tcaaatccat atagactatc tattattatt aattctgaaa atatgttata
    13201 ttttaaagca ggttttataa aacttcctac atttaaaact tcatttgtat atctaaatac
    13261 atcttttaga ttatttacat ctacatcata gttctcaggt tctctagtat ccttatgttg
    13321 gtctacatgc actaacttgc atccttttgt aaactcaccc tttaacaaag attttgccca
    13381 aaaataaaat gcatggttat gattgtcaaa cacatatatc tttttatttc ccaggacata
    13441 ttcgaccata ttctccaatc cttttgcttt tatttcctta ttttcatcta cttcattaaa
    13501 tactacctca ttgcctaacc ttacatcatt taatgtacca tttattagtt ttggaacgta
    13561 tatgctctta ttttctcttt tatcatatga aaatacatta tttcctactg gtttttctat
    13621 ataaaatcca ctatattcat aattttccat aaaactttat ttcctccatt gttatataaa
    13681 tatataagtt ataattttaa aaattaaaat gcacttattg taaatcatat catctattat
    13741 agaaaacatc ttatataact aaatataata tcttctctca atatgtatta tacaataagt
    13801 gcaaaatata tttatataaa atatactaca ttcctataac aaaaccatca gcttcaattg
    13861 gaaggtaact aactcttcta ttcaactcca cctttccaaa aaatactacc caatatggtc
    13921 taaaaatact ttcaatttta actatttcaa tctctgggat tcctcccata aacctatgag
    13981 ttaatctcat agccatttta tttgcctttg cttttactct tcgttcgtcc ttatcactat
    14041 actgcacaat attacaatcc acatttttta caatttcata tgtaggaaaa tcttgtacta
    14101 tactaccaga acctgtagaa ccattcacta aaatttttaa ttcatttata tcattagaat
    14161 atctcctatt cttgaaaatg ggtttcgttt tgtgaatttt aagtgtgata attttaaact
    14221 ctatataatg aagtctaact tcatgagcat atttgttctt taatattagc ttactaaata
    14281 aagaattttt tccactttta gctatctcta tagcttcttc ttttgtaatt cttataggta
    14341 acgctcttat atccataaaa tcattctctt tcttattttt ttcttgtcat ttttaaaact
    14401 ctatatatat aatcaaaaaa tattacaatt acaactgaca taactccata tggaattatt
    14461 tttccaacta taatatcatt tctagtcaat ataccataaa atataaatcc tgttattaaa
    14521 aagtacaata atactagaaa accgtctatt ccaagccatt ttatttctaa atcttttttt
    14581 accttagaca ctaatttaga catagcagtt tctcctttta tagattattt tgtgtattta
    14641 aattttcacc tattctatta ccaatcatcc atgcaataaa tgaaaaaggt atagctatca
    14701 caagaggctc taactcaaag ctctgaactg gaaatatttt aaaataacca aaagtcactc
    14761 caccaacaat cattcctaaa ataccaccca tagatgttct tcttccattt ccaagaagcc
    14821 cacctactat aggtacaaat gccgaaccca tccaaatact tcctacaaat atacatgcat
    14881 catatacatt ttcaaatcta aatgctacgc taagccccat aactgctgct aaacatacac
    14941 ctattctagt aaactttact aattgtttct gagataactc tttttttgta attcttccat
    15001 atatatcatt tgctaatgta gccccagctg ctagataata gctatccaat gttgatatta
    15061 tagaacctaa aattcctatt ataaatatta ctcttattcc ttgtggtaat gtatctaaaa
    15121 ctaaagttat atatccagca gctggagcca tttctggata agtagctctt actataagtc
    15181 ctgtcatagt taacaccatg tcaaaacata tccatagtga aaaacaggtt aataaggctc
    15241 ttcttccaac cttaggagaa tctgctgctg aaaatctttg ataaaaagtt gggtctgaat
    15301 aagctccaaa agaatataca attaacatca atgctttcat agctgacata ccttttgtag
    15361 ggtgcataag ctgtggtgca tctttgagtg cttctgtaag accttgaaac cctcctattt
    15421 gttcaaattg acctggcata accatagtta aaccaaatac catacaaaaa aacaatacca
    15481 tatctgtaat ggcaactccc ataagcccac caagtactgt aagtacaacg actactccta
    15541 ccattattaa tcctattaat tctttatcta ttttccatgc tacgttacct atagttccca
    15601 tagcagttat ttctccaagt tgtgcacaat atataaacat caaaattgcc ccaattatag
    15661 caacatgctt gccataaaaa ctttccagta aatcaggaac tgttatattg gttcttatat
    15721 gaatccttgg accaacccac aatgctaaag gcattctccc aatatgtgca gctattgacc
    15781 aaactgccca tgcagatatt ccatatatag ccgcatactc tacactacct actgtaccaa
    15841 ctcctccata ccatgatgca accaaagttc ctactattaa ggaccatggt aaacttcgat
    15901 ttcctaggta ataactttcc atatctttta catttctaga aaaataataa cctattccta
    15961 ataatataaa tgcaaatata actataatcc cattttccca aaatgttaac atatctaatc
    16021 tccacattct tatattttaa tttttaactt taatatctaa atatgaacaa ctctacccat
    16081 ttccataaaa ataggtagag ttatataata attataagta tttaagtaat cttgttatta
    16141 agcaatttta ttgtttaaac ctttattttc tcttttattt attttattta aagcataagt
    16201 agcaaatatc cctataactg cacatactac agataaaata aatatatatg tatatcctgt
    16261 ttgtccatat ttatctaacc aattacctgc aatagtgtaa taaaacgcat ctggagaaaa
    16321 tcctataaat gaagcaaatc caactactgc tcctgtagtt tctaaagata tattagactc
    16381 tgaaacagat gcaaagtata tacctctaaa tccaaataaa attacactta gtattatcat
    16441 gtttattaca gctatccata taaatcctgt actcttagga ataaccaagt aagcagctgt
    16501 acttactgtc attaatataa atcctacaaa cattactctt atagatgaac caactttatc
    16561 tgttataaca ccagcaactg gagatgctcc catttttata acataagttc ttattattga
    16621 taaagcagaa actagagtca ttggcattac ataaacttct gataagtatg gacttaaata
    16681 agttaaactt gagaatacta aataagttga aaatattata agaccaatta accaagcctt
    16741 tggcattgta actactttta acaatgactt aatacttaat ccttcatctg atgctccact
    16801 atcttttttc tcttctacta agaaaaatag aagtatacca catattatag atgctgcaga
    16861 atataaccat actacatatt tgaatcctac tgttatttca gcaaatttac caaagaaata
    16921 aagacctata aaagaaatta atgtacctga tacgcctgat aacccttcat aaaaaccaaa
    16981 tattcttcct tgctctgact cacttccttg cattctaaca accttaacag aggctgagaa
    17041 gaaggttaat attgtcgtaa atccccataa tacaaatatt aaaagtatca tactatatga
    17101 tgggaatgta gagaaataaa atccaagtat tcctgaagca attaaagaaa aagttattaa
    17161 ttttctaact gagaatctat ctgcaaacca tcctcctata aaataagata ccattcctac
    17221 taatccatat aaactcaata agttccctat ttgtgtatga cttaatgcta aagcttgttg
    17281 cattgggtca taaaatgatg atttcatata tggtaaacta tacatagctg ttgttccaaa
    17341 tgccaatatg aatattatca aaaatctttt aacattattt ttgctttcca ttgattctct
    17401 ctccttaaaa ttttattgtt tataatctca ttcatataat tttattttaa tttttctatt
    17461 gcttgtttaa aatcatttat tatatcattt gaagattcta atcctacagc tattcttatt
    17521 agattttccc cattcatttg aactaatgta tgctcttccc ctaagcttgt tgctattgtt
    17581 gcaagctcta aagaattcat taatcttcta ctagcttggt ctcttgtcaa tccattgata
    17641 ccatccttaa gtttaaatga aactacacct ccacctaaac cattcatttg agttttacaa
    17701 agctcatatt gaggatgaga ttttaatgct ggatacttta ccaattccac attctcttct
    17761 tcctctaaaa attctgctac tgctaatcca ttttcacaat gctttttcat cctcatatct
    17821 aacgttttca taccccttaa aatcatccat gcactcattg gtggtaaaga tgctcctgta
    17881 aaacaacata aacttggcca tctaactaaa tctattaatt cttttgaacc tgctactgct
    17941 ccccctaaag tatccccatg tccatttata aacttagtaa gactatgtat tactaaatct
    18001 gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa aagtactatc aactatagct
    18061 aagcttttat tttcatgagc aatttctgct attgttttta tatctactaa gtctaatgat
    18121 ggatttaaag gactttctat ataaactatt ttagtatttg gtctcattgc ttcttttaca
    18181 ttttgtggat ttactgcatc tacaaatgta acttcaactc caaatttaga taacaactca
    18241 gacatgaata atttagtgtg tgaaaatatt ccatctgaac taactatatg gtcaccactt
    18301 tttaccaatg ctaatatagt agaggttatt gcccccatcc ctgatgtact agatatggca
    18361 ctttctgcat tttcaagcat agccaactta gcttctaact ctgtaagagt aggattacac
    18421 tctcttgtat acacatatcc ccatgtcata cacaactctt caaaatgctc tactgtcttt
    18481 gcagcaaatg ttgctgtttg acatattggt gtagccaatg cacctgttgt tgggtctgca
    18541 ctatgtcccc aatgtattat ttttgtctct aaattttgat taatattgtt actcattttg
    18601 atttcctccc attttgactt atttatacaa cgttttccct gccaaatttc atcaatttag
    18661 cttagtctta atacattttt tcaagtatac ttttttattt gttataattt ctaaaaatat
    18721 gacacttatt ttgttttttt acagttctaa ttataaactc tatagtaact atagagtcaa
    18781 tatgtttttg atattttttt tacaatgttt aaaatagtca atacaaaaat aggtatatct
    18841 tagaataact aaagtcatcc taagatatac ctatttttat atcacaatat aaataattac
    18901 atataagcat tagttttttt atcataaata gatttttcaa gatttatcat tttttcataa
    18961 atatattata gttaaatatt ttaatattac ctaacatcta caataaaata tactcttaaa
    19021 ataaaattat ataattaaaa atactctata aaagctaaaa agtttttcta ttcgctacat
    19081 ctaaaaataa ttagatagag gtataaatgt atttagttta tttatacctc tagtctttta
    19141 taaattatat ctctactaaa ttgatttttt agcacgaata agtgtgctct tggagatacc
    19201 agtcatcttc acaacctctc tatatggctt agaatttaat aaccccaatg catattgtat
    19261 ctgttgattg ctgtatttct ttggcctacc atctttaaag tcctttttct ttttagcaaa
    19321 ttcttttcct aattgagtac gttctattat catatttctc tcaagttctg caactgcaag
    19381 taatgtagtc aaaaagaatt tacccattga agtattttct aaaaggccta tatttaaaat
    19441 atgaacactt acttctttag caaataaacc ttctattagt tgtattcctt ctgatgtact
    19501 tctagctagt ctatccagct tagttgcaac aagtaagtct ccctttttta gagatgatat
    19561 aattttttca aatttaggcc tgtttgtagt agtgcctgta taagtttcct ttattatttt
    19621 agcatcatta tatttattca aaatttcttg ctgttgctga tacaaactgt tgttgtctgt
    19681 ttgatttttt gtacttacac ggcaatatcc atatataata atcatcactt cctaacataa
    19741 agattttgta cagaagttac ccagaagctt gtttttactt atctttatcc attttactaa
    19801 aacgattttt gggtgtcaat actgttaact tttgacacca taataattcc tttataattt
    19861 gtttgactat gtcaaaaatt accaatataa tgaaattaat gagtttatac aaattggagg
    19921 aaataatatg aatagtctgt taaaagaagc aatagatgaa ttaaaaaaca tccattgcgg
    19981 ggaagttttt ttattaaaag aattgttcaa aggctatgaa tggaacaggt tggagatgaa
    20041 tatcagattg aaattaggaa aacttttttt gtatgaagtg gtaactaatc ccaatttaaa
    20101 tgtagaggtc atcaataaaa attctgctta tcagcaaatg tataaaaaaa aatagtgact
    20161 taaatgtata taatagtata atcttcttat tgattcaaaa gttaaaactt ttaatacaaa
    20221 acaatcattc tattttaaca tttatcaata tgtctaaatt tatataatta acacatacga
    20281 atagtattgt ttaaatcaca ttatttaaga tagataaatc ttaaccctac tccagcataa
    20341 atactaaaat agggcttaga attatctata tttttatccc ataaaatgct tagctcttcc
    20401 tcttgattga acatatatat ctggggaaat acaatagaag cgtaaattta taaaatttcc
    20461 aaagtcagaa aacttcagtt caaacttagg tataccaaaa ctttcaggtg ctatttcgtc
    20521 aacaccaaaa tagattataa ttccattatc tgtaagataa aatgcttggt cttcaggtat
    20581 aactaactca acattagaat aatacatttc tctattttca tttatttgag atttaatgta
    20641 atcagtaatt atattaatat aatcaactcc atctctaaaa atgtcattaa gtaaaagttc
    20701 ttttccagtt gtcaaatcta tattgtaatt attcaatgta ttgtatttag gcccatagtt
    20761 attagcaaac cccataaggt ttaatatcat actcagtaca taatttttat taaaagtaac
    20821 tgcgtaactg gtagataccc aatagctttg ttttggaagt gaattttgag gagctgcgtt
    20881 attatattct cgttcttctt cctgtaagcc atttttaaaa ttatttacag tcattcttac
    20941 agcattgttt atgttattta aaataactgg gtttacatac gtggattgat ttctaatatt
    21001 gtatataaca ggatacttta catcatatga aaaatactta cttctttcaa aatcttcatt
    21061 aaattgtaca tataatatat tatatgtaca ggtatttgta gattgataat tttccattaa
    21121 aatcctcccc cttttataaa aaaatcagat acaatataat atgcttaaaa cagtaataat
    21181 gttaatggta aatgtaaaga atagaaagta tctaaaacag caaaaaatga ctaattttgt
    21241 ggtataatac ataggattga gagttatttt ataggaggaa aaatatgtta ttattagcag
    21301 ataaatggaa agattatgaa cttatagata tgggaaatgg agaaaaatta gaacgttggg
    21361 gaagttatgt acttagaaga ccagacccac aggttgtatg gccaatggaa tctgaatggg
    21421 ctttatggaa aaaccctcat ggacattatc acagaagcaa taaaggtgga ggtcaatggg
    21481 aacacaaaaa aagatatcca gaacaatgga ctataaatta taaaaactta aagtttcaca
    21541 ttaaaccaac tggctttaaa catactggat tattcccaga gcaagcagct aactgggatt
    21601 ggatgataga gaaaataaag aaagctaaca gacctataaa agtgttgaat ctttttgcat
    21661 atacaggtgg agctactgta gcatgtgcat ctgctggtgc agaagtatgt catgttgatg
    21721 ctgcaaaagg tatggttaat tgggcaaaag aaaatataga attatcaggt cttaaaaatc
    21781 aaacagtaag atttatagtg gatgatgttg taaagtttgt tgaaagagaa ataagaagag
    21841 gtaaaaaata tgatgcaata ataatggacc ctccttctta tggaagaggt cctaaaggag
    21901 aagtatggca aatagaagaa aagttataca gttttgtaga tttatgtatg ggagttttat
    21961 cagataaccc tttatttttc ttaattaatt cttatacaac aggattttct ccaatagttc
    22021 ttgaaaatgt attgtctaca acaataggta aaaagacaaa tacaggagat atatacggtg
    22081 gggaattagg aataccagct tccagagatg gtaagatact tccttgtggt atatttggaa
    22141 gatgggagaa aaaataatga taaaagtcat atatgaagat aaccatttat tagttgtaga
    22201 aaaaccagtt aacatattgt ctcaaggaga taatactaat gataaagata tggtaaatct
    22261 tttaaaagac tatattaaaa aaaagtataa caaacctgga aatgtatata ttggtttaat
    22321 acatagattg gatagaccag taggtggagt tatggtgttt gcaaaaacat caaaagcagc
    22381 atctagattg tctgagcagg taagaagtaa gactttcaaa aaaacttatc tagctgttgt
    22441 tcatggcaag ataaaatcta actcagatat actaaaagat tttttatata aaaataaaaa
    22501 aactaatatg gtaactgtag tgaaaaaaag ccataaagat gccaaagaag ctgaacttaa
    22561 ctataatgta ttagatttta aagaaaatct aagtttagtt caagttaatt taaagactgg
    22621 tcgttctcat caaataagag ttcaatttgc aagcagaaaa catccattat acggagacca
    22681 aagatatggc aaagaaataa ataaaattgg acaacaaata tctctttggt ccaataagat
    22741 agaaatttgc catcctacta caaaagaaaa aatggaattt acatgtcatc cacctgacaa
    22801 atatccatgg aatatatttt aaatctcaaa tgaggtgatt atatgagtaa aaaattttca
    22861 ataggagaaa tgtctaaact tcataaaata agcatacaga cactaaggta ctatgaccaa
    22921 atagatttat ttaaacctgt tcatgttgac caagaaagca attacagata ttactcaatt
    22981 gaccaatttt cacaattaga tattataaaa tatttaaaat atttaggtat gcctttaaaa
    23041 gaaattaagt caaagcttga tgaaaataat gaagaaattc taaaattact aacaaataaa
    23101 attaatatag tggatgaaaa aattaaagaa ttagagctta taaaaaaggt actaagcaca
    23161 aagaaagaaa ctatggaaaa aggtatgaaa gtagatgaag ttggtaaaat agtgagaaaa
    23221 catattccac gaagaagtat cttatctata gagtatgaag aaggattaag tagtgaagaa
    23281 actatggaac tttccagaag acaaatagca aatatattag aagataatat atctgtgttc
    23341 tatggtggag taagtggact tgtatctata gataaatttt taaaagaaga taaagttatt
    23401 tatgacaata gttttgttac tgttgaaaga gacttatttg atataaaatc acaaaaacat
    23461 ataacagaaa tttcatctgg tgattttatt tgtatgacat ataaagggcc ttacaaagaa
    23521 aattataccg cttttaagaa actggttgat tatgttgaat ataaaagaat acctattgaa
    23581 aattatatat atgaaatacc tataatagac cctctgtcta caaacaacga agaggaactt
    23641 ctaacagaaa ttcaaataca agttaaaaag agagaaattg acttaagatg tctagtgtag
    23701 taatctagtt tacttacaca tcttaatata gttgtctatt atcaacatat gatttttcta
    23761 gatagtgatt cctaacagtg agattttcaa aaaataggag gaaatatggc taatgctaat
    23821 tccctcctat ttttaattaa tccatatcat tttaattaag ccatgtcatc acgaagtgca
    23881 tcaataatat tttctttttt tatcttatta atagtataca acattgtaat aaatactata
    23941 aataaaacac taaatatact aattccaata ctaaaccatg gaatcacaaa atcaatatca
    24001 tctgtaacca ttactttatg aattagccat gaagaaatta ctgctatagg aagtccaaag
    24061 aacaatgctc tcatgccata aaaggcacac tcaaaattca tcatcttttg gaaatcatgc
    24121 tcagacattc ctacagagcg aagcatggca agttctctac gacgtaactt aatattagtt
    24181 gaaattgtat taaacacatt ggcaatcgaa atcagagaaa tcataattat gaaagtataa
    24241 gcaaacacat tggcaataaa aatgtaatta cggttctcat ccatcattcc atacatattg
    24301 tacagattgt actcattcgt aactccctca ctctcaataa tttgctccat ttctgcaact
    24361 gactgagtag gatttttcga ttgaaagctt aaactttcaa ttcctgtaga aacaccagga
    24421 atctcaaact tatctttggc tgaataagga gccaatactc tgaagcttgc tttcttcaca
    24481 tctgaacttc tgattcctgg aggagaatca ggtggcacta ctttgacaaa tgaaatattc
    24541 acattttgtc tctcaggtat ctccaactta ccattagttt caggagtgag gctaacattc
    24601 atagaagaac tgttgaaaat atcaataata ttattatcct ttgttaccat agaaacagca
    24661 atcagttttg cattttgtcc agtatattct tctgctggta atccaagact tttaataata
    24721 ttcagataag ttctatcatc aagaaattgc atatctactg gtaattcaag catttcactc
    24781 atagaatgtg aaccctcata gttcaaataa tcgtctgtaa gctcacttac tttggcaaca
    24841 catgaatagg tcataagttt ctgatacaaa ccttcgtaaa caccatttgc agtttttagc
    24901 ttatcgtaaa gcttcaacat ttcgttgtct ttcatacctt gtgtagaaaa aataatatcc
    24961 tggtcagtaa cctctgacct ctgatttgac atctgtttta ggtctgctac taaagcactt
    25021 gtagatataa ataaaactat gcttaaaaca agcgacaata caatacttcg ataacgtttc
    25081 ttgtttcttt taaagttttt catagcaaga actccctcaa gaccataaac tcgctgtgtt
    25141 agttttgatg tcttcacaga tttagattca atttttacct catttgtctg acgaatacac
    25201 tccatcactg gtgtgttggc agcctttctt gctggtatgt aagcagaaat taagattgta
    25261 accatactta ccactgctgc aacgatgatt gcagggatag acaatgttaa tgtcaacgga
    25321 acgtttacat agagaatatt tgcaaagttt ttagctatga cagaaatcac aagtccaata
    25381 ctgactatac tgattgcaac accaattggt ataccaatcg caccaatgca aattccttca
    25441 aatagcaccg aatttcgtaa ctgttttgct gtagctccaa cagatgaaag tattccaaat
    25501 tgatgtgttc gctcgttcaa tgacatgtta aatgaattat atattagaaa aattgagcct
    25561 agcatgatta tggcaagcac aatgccacca accgagtaca aaaacatatt gaacaaattg
    25621 ttatcagaaa cacctatgaa gcgtaacaca ttgtcattga aaatggaagc atggtcttta
    25681 gtgctacttg tgtaagagcg aatctgatgt ggatttttca atgtgacaaa aaggctaaga
    25741 ctgtcagtct tgacttgttt atcttctttg gttatcaagg tatatcctgg tgcagaagat
    25801 tcttcaaata cgggctttct acagataccc acaactgtat aagttttctt atcctgtggc
    25861 acaaaaactt cactcccagc agtgtatggg tcactctgac caagcttttc attttcactc
    25921 attcgtttcc ctacatccaa agaaatagta tcacctactg caaatttgac accaccatct
    25981 gtcgcaactt tacttgaaac aagaatctct ctactatttt caggtaatct tccagaaatt
    26041 aaagttatag gcaaagtatc aaaagcttct ttgctaaatc cagcaataaa aagatatggt
    26101 ttatctggat ttctgttacc ttcaagtgtt gcatagccaa tattttcaaa tgttgcagta
    26161 tttgcaactt catcattgac tctttgttcc tgtacaaaag aagaatccac atccaaaaac
    26221 tggacatgcc aatcaccata ttttataata gctccattta tcatatagtt catcaaagaa
    26281 actccaaagg tagtaactgc tgtaatcata gcagctgaca gaacaactcc tataattgtt
    26341 acaagagttc gagttcgatt ctttttcaat ccttgcagag caactttgtt aaaaatgttc
    26401 attaacgatt caccttcctc tcatcacgca ctacttttcc atctgaaata cagataattc
    26461 tatctgcctg taaagcaatg ttttcatcat gtgtgacaat aatgagagtt tggtgatatt
    26521 tactatggct ctctttcagt aactggataa tctcttgtcc atttttactg tctaagctac
    26581 ctgtaggctc atcagcaagc ataactgctg gtgcattcat caatgcacga ccaatggcaa
    26641 cacgttgctg ttggccacca gaaagttgat ttggtaaatg tgtttttcgt tcttttaaac
    26701 caagtaattc taacaagtca tttaaccgtt cttgattgac cttacgctta tccataagta
    26761 taggcaatgt gatattttct actacattta atgtgggtat cagattgtga aactgataaa
    26821 tcaatccaac ttgtcgccta cgaaaaatag caagtttttc attgctttgg ccatatacat
    26881 cttgcccctc cagatatatc tttccacttg ttggtacgtc tactcctgca atggaatgta
    26941 gtagtgtaga tttaccagaa ccagaagaac caatgattgc agtaaactct cctttttcaa
    27001 tagtaagtga gacatggtca agtgcagtaa ccttattttc attcttacca taaaccttac
    27061 ataaatcttc aatctttaaa aacgccatta tataagtctc ctttcatatt ttataaatat
    27121 atcataggac tttcaactta catttaagtg acatttaaat gagagattcg tcactttgga
    27181 aaacgaataa caaatattgc accaccttgt gggtgatttt gtgcagtaat tgttcctcct
    27241 tgtctcataa taatcgtctt acaaagtgcc aatcctattc cataaccggc tgtgcttgaa
    27301 tttttcccac gataaaatct gttaaataga catggtaaat cttctttttt aaagcctgct
    27361 ccgttgtcat gaatagtaag ttgagtaaac aaaaagttgt cctcacaaat gatatcaatt
    27421 tttccattat cacccacact ctccatgcag tttttaaata tattttgaat tgcttctgaa
    27481 agccaattta aatcacccaa aatccttact ccttttggta tatcaatatg taaagtgata
    27541 ttgtgcagtt ccatgggaat caaaagttga tgaagtgcag aactaatcaa attattcaca
    27601 tcaatttgtt ctttttgaaa gactacaata cctgcatcta aacgtgataa ttttaaaagg
    27661 gaagtaatca gccagtccat tcgtataagt aattcctctg tttctcgtat caactctttt
    27721 ctctcatctt catcagaagt attctctaac agtgacaaaa taagagttac tgatgtaaga
    27781 ggagtgcgaa gctggtgggc tatgtcagct agtgagtcag caaggtgttc tttttctttt
    27841 ttcaatgcgt aattttgctc tctaatacgt aacgtcattt tagttatttc actttgcaga
    27901 atagaaagtt caccctcatc tgattcagca atatacagat ggttagcatt atgaagcaca
    27961 agattaattt gttctgaaat ctgtgcaata cttttgtatc gatttttagt aaacacaaaa
    28021 aatgctattc caaaggtagt ggcagaaaca atagaaagta ttccagtcat aatactgatt
    28081 gcaaatccca gggctacagt gagaatagta attaataaga ataaaatagc aaattttcga
    28141 aactctctat ttcgaaacat aacttccccc caatctatac cctgtcccac gaacagtcaa
    28201 aattatttgt ggacttgagg ggtcatcttc tatcttctct cgcaagcgtt tgatgtacac
    28261 agtcaatgtg ttgtcattaa caaactcacc tgcagcatcc cacaattcat caagtaatct
    28321 taccctcgta ataatacttt taggattgct aataaacacc aataacaaac ggtattctaa
    28381 agctgaaaga aaaacctcat tgccattctt tttcacaaca ccggttgctg tatctacata
    28441 aagcccacaa atttcaaaag ttgacccaaa acgtccactt tttcgtagtg ctgttctaat
    28501 tcgcgcaatc agttcacgtg gacgaaaagg tttagtaata taatcatctg cacccatatt
    28561 cagtccagta acaacacttg attcatcacc agaagctgtc aaaaagataa cgggaatgtc
    28621 ttgcttttct ttgatttctg tgcaaatcgt aaacccattt ccatcaggta aagaaatatc
    28681 aataagtgcc aaatcaaatt tattcttagc aatctctaca agtgcttcac ttcttgtgga
    28741 tacatgggtt actataaatc cttctgaatt aagcaaaaga ataagatttt tggcaattgc
    28801 tttatcatcc tcaactaaaa atatccgttt catttacttt catcctcctt tattttatta
    28861 gttttatata ttccagcaga gcaatagtac actactattt tacttatcat tacacaattt
    28921 tcagtagttt cttttgttct tatataatcc atgcataata cctccattga tttattatat
    28981 ttcttataac ataaatacac aagataagac aaaaaacata atgtaagtgc aagtttttta
    29041 actcactaac ttataaacat acactcctac taacactact ttcttgatga cacatcttac
    29101 aagcttatat tcatacatca atgaaaaatt aaatttatcc atttattaaa ataaaaggca
    29161 atagcttcta tagctaatgc cctttatctt aaaatgattt aaatactata caatttttat
    29221 aaatggctat aaataaactc gtatagaata taaaatttat ctacagtctt ttgttataca
    29281 aaatttataa cctcttatag aacaaattta ccactttgca aacttatcac ttttactcag
    29341 ctatattata aacttttaat ttatctttgt ttaaatgttt cttcttaact tctactaaag
    29401 cttttagcgt atccagagat gtcattattt cagtagaaaa ttcaatagct gttcttctca
    29461 ttttaaagcc atctcttgta gagtcatttc ctttagttgg tgtatttatt actatatcaa
    29521 cttgtttatt tcttatagca tctaatatgt ttggtctagg ctcttcaatt ctatttacta
    29581 tatcagcctc tattccattt tctcttaaac tcttcgctgt tccttctgtg gcaacaaaag
    29641 tatatcctaa ttctttcata tcttttgcta tctctataaa ctcatcttta tcatgattgt
    29701 ttacagtagc aagtactact cctctttcat cagacatatg tctacctgct gccaagaatc
    29761 ctttatataa ggcttcttct aaattttctc ctacacctag aacttctcct gtagacttca
    29821 tttcaggtcc tagacttact tcaactttag ataatttaga cattgagaat actggtactt
    29881 ttactgatac taattttggc tctttgtaaa ctcctgtacc atatcctaaa tcctttaatt
    29941 tagctcctaa catacactta gtagccaagt ca
//
LOCUS       DAELJO010000024        29904 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELJO010000024 DAELJO010000000
VERSION     DAELJO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.024"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            23..511
                     /locus_tag="KPB88_000987"
     CDS             23..511
                     /locus_tag="KPB88_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053285.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
     gene            1045..1722
                     /locus_tag="KPB88_000988"
     CDS             1045..1722
                     /locus_tag="KPB88_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG0053286.1"
                     /translation="MLKILQDKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     QTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYAISQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            1866..2033
                     /locus_tag="KPB88_000989"
     CDS             1866..2033
                     /locus_tag="KPB88_000989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053287.1"
                     /translation="MSDGKICVSCQRTIADVATELTIAYMENASLTPEKIAEIYKLFY
                     KNANEAFNGKF"
     gene            2100..2789
                     /locus_tag="KPB88_000990"
     CDS             2100..2789
                     /locus_tag="KPB88_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0053288.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKYNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMIKEIKKHESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLNKDDTDKEITV
                     RVMKASGENGK"
     gene            2779..4122
                     /locus_tag="KPB88_000991"
     CDS             2779..4122
                     /locus_tag="KPB88_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0053289.1"
                     /translation="MESKFISNDHFYDFIFNWDYKFYFLVGGYGSSKSYHVATKLIIK
                     LLEEKRKALVVREVYDTMRDSCFSLLEEVAESMNLGDILSFKTSPMRVIFPSGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVTKNNTYYHHSVADDNFFLPKSYIEQLDEMK
                     EYDYDLYRIARKGRFGINGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQEPIKADAAEPKTIKYF
                     RQQGFNISGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYGLDGYEVSDIKELKYSNDIYNKGLGLKKNNQYNKKGGTVF"
     gene            4122..5645
                     /locus_tag="KPB88_000992"
     CDS             4122..5645
                     /locus_tag="KPB88_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0053290.1"
                     /translation="MNIKNILLNLDEKELRDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIVDNKVKPLINKQARFMFGKEPTIILKAYEKEHKEACEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSINDFKYQVDSN
                     DITKLKSVVFVRFDSSTIKEVTAKQIWYRYTYYMKKSDVSNQESCFIKIEKFKGDNLS
                     KPIKITENDTKLSKIPCWVIVNEQSITNIRGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKAKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDNIDEELNSKSNNSDE"
     gene            5658..7277
                     /locus_tag="KPB88_000993"
     CDS             5658..7277
                     /locus_tag="KPB88_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053291.1"
                     /translation="MKDNTYTKKVLEARKKLLLLDKKVQLEILSVYKDASKTILSDII
                     KNKELNLSTKYLKKLNKLIEKYINEPNQRLVPVIEKSITEASNIAKDLQMYYYQSIVP
                     NKSINLACDAMCIKTTTNVVGKIVAGNFYKDKRSLDSRIWGYSNKNRKDIDRLIKANI
                     ARGANAKTLAKSLDNYVNPVKKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCVGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRFGLGEGVFPPEKYPIPHP
                     NCLCYPVQVIIPISEASKMMENWLGGGDDDILDSWYDEIREKNNSAPIIKKQRNKKNK
                     ITANSKGDRIKKNITNSIKNKSKKAPKQIQKYINKYVNPNRIIIDNSQKIPFVYYTKV
                     DLVGINPNIREFKSYNKEAALLHEFAHRIDINEIKSYNNIKFQQAIESSSMYVIKNIE
                     NLQSIYTNSNELYNNEFISDMLGALSNNEFEDLLATHSEKYWSKNRNKEKEIFANLFT
                     LNYQNNKEINSFIKEHLNSLDKIFNELLGGI"
     gene            7279..7431
                     /locus_tag="KPB88_000994"
     CDS             7279..7431
                     /locus_tag="KPB88_000994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053292.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            7499..8152
                     /locus_tag="KPB88_000995"
     CDS             7499..8152
                     /locus_tag="KPB88_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0053293.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRYVEKTE
                     LENANKEIKEYKKQMGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIRESAEKEIE
                     VLKFNTAFERVIESYNPRNSKALAALINKDNISFVNGKFIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDEIGGTGGLEGGTTSLLDNDSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            8164..8520
                     /locus_tag="KPB88_000996"
     CDS             8164..8520
                     /locus_tag="KPB88_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053294.1"
                     /translation="MSIEKSEIYMGENKTTLKFAGNLFQNVNIKVKKTDVAIVDGKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRNIDFTYSNGNESIPVLIFGFVDEKTLPS
                     VIPEEAKQAMKMIMFL"
     gene            8544..9572
                     /locus_tag="KPB88_000997"
     CDS             8544..9572
                     /locus_tag="KPB88_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG0053295.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGNPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDRDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            9589..9789
                     /locus_tag="KPB88_000998"
     CDS             9589..9789
                     /locus_tag="KPB88_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053296.1"
                     /translation="MAKKKENLMQVKALVYLKYDNDCYKIDDIFEIRKTEQELMEEKG
                     YIEVIGEVEEKNNNELLNKDGE"
     gene            9792..10109
                     /locus_tag="KPB88_000999"
     CDS             9792..10109
                     /locus_tag="KPB88_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053297.1"
                     /translation="MSITDLDKLKLNLQEEEYPYFTDEQLVMLLESNENNVLKASWRG
                     CLLKGATDDEIKIGPIETKSSNSSYWLTLADIYKTDYLEEKSKNETTNTGYKTSMTRA
                     DGQ"
     gene            10106..10489
                     /locus_tag="KPB88_001000"
     CDS             10106..10489
                     /locus_tag="KPB88_001000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053298.1"
                     /translation="MRKLRADKIIKTINRGIALNPQTITIEQEIKQIVDGAIDITNEV
                     KELTVVIYPEKTNDTVISSETIGTAYKNKNFGMVADKEADLRLNTESDITFKCIEGTM
                     KLNYVNTVVVEEQVCGYICGLKKLD"
     gene            10502..10912
                     /locus_tag="KPB88_001001"
     CDS             10502..10912
                     /locus_tag="KPB88_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053299.1"
                     /translation="MSVFTKAINEIDRKKATMPLLCMNIAFMLEGEAKNSAKWTDRTG
                     NARQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHIIKPKNAKALFWNGASHPVM
                     KVQHSGTKGTRSLESTVSKNISKIGKALEKHWSD"
     gene            10915..11190
                     /locus_tag="KPB88_001002"
     CDS             10915..11190
                     /locus_tag="KPB88_001002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053300.1"
                     /translation="MKDNLDDMKLTNVTLDYCEEKRIGKLIIDGVDFSNKATSVHLDF
                     EGCSIPIIKVELVCDEIKLNGDFIVKKDIDKSIEKKVISEMFNRWSK"
     gene            11191..12015
                     /locus_tag="KPB88_001003"
     CDS             11191..12015
                     /locus_tag="KPB88_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053301.1"
                     /translation="MRAGIRKALIENIPRIKDCYEPTVPNKKTIKPYIVIVQGEDADN
                     EGNVIGFRRTINIWLYEKRTTFNKLDELTKEVIETLDFKTIIDDTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALDDKEDATSDRWVEALSKYTKDLLEIESYKDNWKKN
                     FIAPCALWRTTNVKNKRINYHLIEITKTMKCHVVSKNKDEIVKLLETLETHLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFSSGQLTVVFKMIGKIKREGPIMNEIYNSGNLR"
     gene            12032..12232
                     /locus_tag="KPB88_001004"
     CDS             12032..12232
                     /locus_tag="KPB88_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053302.1"
                     /translation="MAETSNKKTVSKQEEKYSKEDFLKNSEALGYEKMVVAGALFNCK
                     KKELTKVEFEKAIKDFLEREVK"
     gene            12234..13655
                     /locus_tag="KPB88_001005"
     CDS             12234..13655
                     /locus_tag="KPB88_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0053303.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAIPIRAN
                     WGDVGKVVTIKNDLRQLKTSFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVVATKVADSDTTLANVVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKKEDNIKQINDKS
                     KGFNDENIVNIGSSAYYEGIKYTPSEVAVYIGALAVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            13671..14084
                     /locus_tag="KPB88_001006"
     CDS             13671..14084
                     /locus_tag="KPB88_001006"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053304.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            14168..14662
                     /locus_tag="KPB88_001007"
     CDS             14168..14662
                     /locus_tag="KPB88_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053305.1"
                     /translation="MSEIYKRELENEVVEEDNDLDEEIEETNEDRLKMKEDEIIAKLL
                     EDSPVPQRTVFLDRLGIPITLKALTEKEISKIRKECTKIVKVQGRREEKLNDDEFTLA
                     LIEKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDD
                     DIKN"
     gene            14839..19116
                     /locus_tag="KPB88_001008"
     CDS             14839..19116
                     /locus_tag="KPB88_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0053306.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMRA
                     LNRIKANPVIQAQDKTSSVVNRISNNLKRVGRTISTTINAKDRASSVVNRVKNKVNSL
                     LTSRQREVLLKARDKASQVVDKVKAKVQNLTAATIISLNMKADPALRVISQTRSKLGE
                     LKNNTIINIKAKGEEALNTISRTKSKLQEFVSKRYEAAVKIRDEASSALGGLTGKINS
                     FVSGAISKFAQLTTAITVALGGIGVGSSIKTFANFEQGMKNVQAVTGASGKEMEALTV
                     KARNLGKTTAYSAREVSDAMYYAGMAGWKTNQIIEGMPGILNLAATGGTDLALTSDIV
                     TDGLTSLGMAAKDTNEFVDIMAATITNSNTNVELFGETLKFIGSLGGSLGVPMQDIAL
                     ATGLMASSAIKGSQAGTSLRMGLLRLINEPKRAATAMRKYGIEMKTTKSGSLDLAATI
                     DELRNKLGKLSDTKKVGALGDIVGANASSGWAAIVNASESDYNKLKKAITESEEEAKR
                     IADMKMDSLNGQFAKLKNTINDVKISIGEKLGPVTRNFMENIISSMPKVGDSIVNFVS
                     NFIKNFDKIKSVLQGVISIIGGVVAGFMAFKALTVISSVISIITKIATASTLIGGIVG
                     VLGPVGAIVTATGVLAGAFLFAYQKSETFRYGIKNIGKSISNFLKPLNPFIEQIKGKF
                     KELINALKPLFAVFSKLGSMIVSALSPVIQFLATVFIIKFMYLFNVIVNKVKAIVNTT
                     TVVVNGLIEIFKGIADVVKGFINRDMSQVANGLKSIFKGIIDIVKSLWSGLVDFVTSP
                     IQAVVDILDTKFGKKVEGIKKKWNELKDFLKNPTKAAPKVQPVNLSSEKASSELQTSS
                     NGAKAYISSLGQKIGEGIGKIKDKFGELKISATEVFNNIVSFVGGKATELKDKLLEGI
                     KPAIDTFKQSLSNLKEVFGDSLDSIKEAFGGLKTVFDENIKTPFENLKQKVLETKESL
                     KPVFDNLKSSFAELGKALEPIKEAFSGIKDFFSNLFKPIKDDGATKTTKTNMDELKQS
                     TQRVGTSFKELGNAFNQLKEAAKPFIDYLKQIKDSLTSTLGDIGGGLLKGVATSIVLV
                     ITSVINAIASIINAVAGAIKGVIDIIKGIFEIIGGIISGDGEKIKQGFSDVFKGIGEV
                     VKSLWEGIKGVLGAPLKAVVNFIDNGFSEKVGQVKQWWSDLKTNVGQKISGFVSFVSN
                     GFQQKVQQVGMWWQGLKVNLSGKISGFVSLVENGFKSKVDSIKSAWDSLRKKLSQKIT
                     GFVSIVKTGISNVLDRFADGGVASKPSICGEAGPEMVIPLSNSKRSRALSLYEQAGQM
                     LGTKASNNVIPISQKLGTSFNSASSIQNSNSSIINNVRQFPLTNALDTDNKEYKEATP
                     NNVNSSNTINLGGISINIQESNNKEDMIQEILSQVESEIREALQDIG"
     gene            19196..20116
                     /locus_tag="KPB88_001009"
     CDS             19196..20116
                     /locus_tag="KPB88_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG0053307.1"
                     /translation="MNFFKRKDDIKNFKLMYIKGHPHFNNELTCTMYIDKKLVSVYTL
                     QQVPISEIKLLDIEKVFIDNDMLIIESKKSVLYFKSNSKDKICNIINQNIADMQDKKT
                     DTILRNKNKIVKQGVMKMDYYNSVGVPNCRGEAISIIIDNHNACLVIESKLLKEEKMR
                     IKFSDLYSVNKYSEKEIAYNNKSVVGRAAIGTLFGPMGTIVGGLSGTGNKKSKTEKLF
                     ISIEYFSHGELREIIAEDSILNFHSQEFIDNIRRKINISKNNLENNKEKQIKSNDDAI
                     QQVKGLKELLDMGAITEEEFNKKKKELLNL"
     repeat_region   20374..20796
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20374..20402
                     /rpt_unit_seq="attttatattaactatgtggtatgtaaat"
     gene            20955..21122
                     /locus_tag="KPB88_001010"
     CDS             20955..21122
                     /locus_tag="KPB88_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053308.1"
                     /translation="MPSKKIGRPTDNPKGSRITIRLDEESKNILEKYCEKEDIDKAEG
                     VRRGIKLLKDK"
     gene            21246..22073
                     /locus_tag="KPB88_001011"
     CDS             21246..22073
                     /locus_tag="KPB88_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG0053309.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAICEIIKKIKL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            22126..22302
                     /locus_tag="KPB88_001012"
     CDS             22126..22302
                     /locus_tag="KPB88_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053310.1"
                     /translation="MYENLLDDYNLKTDKERKDFIKFAILLYKLEQNDKEKFYGYAEI
                     LKEILREQQERENN"
     gene            22399..23604
                     /locus_tag="KPB88_001013"
     CDS             22399..23604
                     /locus_tag="KPB88_001013"
                     /inference="COORDINATES: protein motif:HMM:TIGR01766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0053311.1"
                     /translation="MIAVKKLKLTIVEEEEKRKEQYKFIRDSQYAQYQGLNLAMGILT
                     SAYLASGRDIKSDLFKDSQKSLTNSNEIFNGINFGKGIDTKSSITQKVKKDFSTSLKN
                     GLAKGERGFTNYKRDFPLMTRGRDLKFYEEDKEFYIKWVNKIVFKILIGRKDKNKVEL
                     IHTLNKVLNKEYKVSQSSLQFDKNNKLILNLTIDIPYKQVDEIVKGRVCGVDMGIAIP
                     VYVALNDVSYVREGMGTIDEFMKQRLQFQSRRRRLQQQLKNVNGGKGRKDKLKGLESL
                     REKEKSWVKTYNHALSKRVVEFAKKNKCEYIHLEKLTKDGFGDRLLRNWSYYELQEMI
                     KYKADRVGIKVKHVNPAYTSQTCSECGHVDKENRETQAKFKCLECGFEANADYNAARN
                     IAKSDKFVK"
     repeat_region   23951..24372
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23951..23979
                     /rpt_unit_seq="gttttataatgactatgtagtgtgtaaac"
     gene            25030..25701
                     /locus_tag="KPB88_001014"
     CDS             25030..25701
                     /locus_tag="KPB88_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0053312.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVTPLDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIMT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            25698..26660
                     /locus_tag="KPB88_001015"
     CDS             25698..26660
                     /locus_tag="KPB88_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG0053313.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLIEPKQLKDI
                     NIKKGDKIVLIDIAYESKKEETIFDGVIWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     SFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDSDKYGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKNKEYYVIDVTHTLDSRPKMKLNIGTLEYIRRKFYTND"
     gene            26653..27018
                     /locus_tag="KPB88_001016"
     CDS             26653..27018
                     /locus_tag="KPB88_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053314.1"
                     /translation="MTDARFNGIARILKENMNKSVANCTFGMGCELAEITTNGLKVNG
                     YKDEIQDYLVLESLILKEDYFTFSDEALGGEYRHKHKLETPKELKPLTIGDKVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            26993..27451
                     /locus_tag="KPB88_001017"
     CDS             26993..27451
                     /locus_tag="KPB88_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0053315.1"
                     /translation="MPNLFPQSETFETVELKNNNENELDLKGSFLFDFIKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRHKRSAYSNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVECEFNVLTIDDENIVIDGNIKR"
     gene            27460..28593
                     /locus_tag="KPB88_001018"
     CDS             27460..28593
                     /locus_tag="KPB88_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0053316.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKAIGTITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGANEKVDIKAECRIAGTIGNVSNNTITVL
                     LGSISGIKSVTNKEDLKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWAGAGTVKVLILDKNRKAATQELINKVQEYIYPNVEEGQNRDGKAPIGAI
                     VTIATPQTLLINVKASFVFSNGFSQETVLNILKNKIDKYLDKIDIGGTVSYNAIYSLA
                     GSMMLADEGIQDFSNLTINDGTTNIILNDQVVGIGEIINEVIA"
     gene            28590..29360
                     /locus_tag="KPB88_001019"
     CDS             28590..29360
                     /locus_tag="KPB88_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0053317.1"
                     /translation="MITSKKGREMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLDEE
                     IELQLFPQSATWGLEFWENRVGLYTNIDEDIEARRRKVIAKLQMKYIVNPDRLATIIK
                     SYTGADVYIKENIAPYVFKVTANVDDVINYEDFKYITNKTKPSHLHWMPSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWLLNGSKNLSAYILYNEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGIEMLNGNKLLNAELREEVL"
     gene            29361..29741
                     /locus_tag="KPB88_001020"
     CDS             29361..29741
                     /locus_tag="KPB88_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0053318.1"
                     /translation="MANAVTTDIARQKMCKARAGDIVLPTIVSMVFGDGGVGNDGTII
                     APLSSDTALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKTFGNKYKDSDMEMVFQIDDEF"
     gene            29753..>29904
                     /locus_tag="KPB88_001021"
     CDS             29753..>29904
                     /locus_tag="KPB88_001021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053319.1"
                     /translation="MPNELNFNNEIEQYLITTPAHANEFNNRQQKLLDNDKYLNNKID
                     TTKTEL"
BASE COUNT        12409 a         3108 c         5605 g         8782 t
ORIGIN      
        1 acataaacag gagtgtggaa gtatggataa taatatcaat aaaaaagaac tatttaaaaa
       61 agtagaaggt agactacatc attataaatt tttaagtgca gaaattaaaa atcttgaatt
      121 agatatagaa agtagagaaa atgagatatt tgggtgtaag gctgttggat atgatgaaaa
      181 agtaagtcca acatatgctt ttaattcaag tgtggagaat gagattataa aaaaagaaag
      241 agatattact agattgaaaa aactgaaaaa agataaggaa attgaaaaga agaaaataga
      301 aaatgcactt acatgcttag atataagaga agaacatttt tttaaactgt tttataatag
      361 cagaatgaaa aatagtatgg tttatatatc cttagagatg aactcagata gaaaaacatg
      421 tagatgtgtg agggaaagat tagtgtataa aattatggat atgctttatc caagaattaa
      481 ggaaaatgaa ctaccattat ttaaaaatta gaaaattccc cagtttttcc ccagaaattc
      541 ccactttatt ccctactttc tccccttttt gattaaaaaa gcatgagata atagtattgt
      601 ggaaataaag atttccctct caaaactaaa taattgctag gttagtttaa agggctaatc
      661 tagcaatatg aacagactag gcagggcgtg aggacgctgt tagttcaatt ctaactatgt
      721 tcaaaatcta ttgatacact atattaagta tttgaattaa gattaaaatc tcatacagtt
      781 ttgtatctta attcagaaat ctaaaaaatc gggtggggct tggtaacctc actcaccatg
      841 caggtagtgg tttaatctag gttcgattcc tagaacttgc tcccttaata atatgtatcc
      901 cccattaaaa aggcttagat taatttctag gtctttttta tacaaaaatt taataagttt
      961 tagatatcag taactctgat aacagaaaat aagggtgttc aagttgagca ccctttatta
     1021 ataactaatt taggaggtag tagtatgttg aaaattttac aagataaaaa tgtaaaagta
     1081 atgtggtcca aaaatggaga agaagtttgg tttaatgcaa atgacgtagg agaggaacta
     1141 ggcatagtaa atattcgtga tacattaaga aatatagata gagaatataa aaagaaattt
     1201 aatgagtcta ctgtcggaga ttcctacact agaaacttta aagataaatt acctaacttc
     1261 ggtactactt ttgttacaga agaagctgtg tacaatatgt catttagaag taataaacca
     1321 gaagcaaagt tatttacaaa atgggttaca caaacactta aacaaattag aatacatggt
     1381 tactacattg ctacagaaaa agaccaagaa tggttggata taagaacaga aggtaaaaaa
     1441 gtaagaaaag attttacaga tgaaatacaa gaatttgttt attatgcaat tagtcaagga
     1501 agtaataaac ctcagatgta ttataaacat tttaccgaac ttgtaagaaa aaaattaggt
     1561 ataccaaaag gtgtgaaaag ggatgaatta aatcaaagtg aattgtttga tatacaagca
     1621 ctcgaaagaa ttatatctat gaaattacct aagctaatag ataaagatat gaattataaa
     1681 gaggtatata aaaagattaa agagttaata gaaatgattt aaatgactgt cttgatggat
     1741 ggtctttttt tatataataa attaaataag tgtattattt agaattattt tgtagttgtc
     1801 gaatagtttt tgaaggatat tgacctttga agttgaatta tatactttat aaggaggtga
     1861 ctattatgtc tgatggaaaa atatgtgttt cttgtcaaag aacaattgca gatgtagcta
     1921 cagagcttac aatagcatat atggaaaatg cttctttaac tccagaaaaa attgctgaaa
     1981 tatataagtt attttataaa aatgcaaatg aagcgtttaa tggtaaattc taaaagatta
     2041 agaactctaa ccagagttct ttttttgttc ccaaaacgac aaacaaacga ggtggtggta
     2101 tgaatgaaaa ggcagattta gctcatgaag attacttaaa agggcttaag tacaaggaaa
     2161 tagctgaaaa gtataatgta agtttatcga ctgtaaaatc atgggcaact agatactgga
     2221 aacaaaaagg ttgcaaccaa tcaaaaaaag ttgcaaccaa aaagagaggt gcacccatag
     2281 gtaataaaaa cgctactggt ccaccaggta ataaaaatgc tgaaaagttt ggtttcttct
     2341 caaagtatct acccgaagaa actagagaat tgatacaaga aatatctata aaagataaat
     2401 ttgatattct ttgggagcaa ataacaatcc aatatgcagc aataataaga gcacaaaaga
     2461 taatgtatgt taaagacaaa gaagaaatga ttaaggaaat caagaaacat gaaagtacag
     2521 aaaatggtga gaagatagag tatgaatttc aatttgcatg ggataggcaa gcatcttttc
     2581 ttaatgcaca gagtagggca atgagtgagt taaggagttt aattaagcag tatgatgaga
     2641 tgattcataa ggattggaat ttagctacag aggagcagaa aaatagggtt gagaagttaa
     2701 aatgtgaggt tgataaccta aataaagatg atacagacaa agaaataact gtaagagtta
     2761 tgaaagcgag tggagaaaat ggaaagtaaa tttatatcta atgaccattt ttatgatttt
     2821 atatttaact gggattacaa attttatttt ttggttggag gatatggaag ttcaaagagt
     2881 tatcatgtag caactaaatt aataattaag cttcttgaag aaaaaagaaa agctttagtt
     2941 gtgagagaag tatatgacac aatgagagat agttgttttt ctctattaga agaagttgca
     3001 gaaagtatga atttaggaga tatattatca tttaaaacaa gccctatgag agttattttt
     3061 cctagcggaa gtaaaataat atttaaaggt atggataatc cagcgaaatt aaagtcaata
     3121 aatggtgtat ctataatatg gctagaagag tgttcggaag ttaaatacac tggttttaaa
     3181 gaactcttag gacgtttgag acatccaagt ctatctaatc atataatttg ttcaactaac
     3241 ccaattggcg aagataactg gacctataaa cattttttta gagatgaaga aaaagaccga
     3301 tttgtattag atgatactgt attatataaa aatagaatag taacaaagaa taatacctat
     3361 tatcatcatt cagttgcaga tgataatttt tttctgccga aaagttatat tgaacagctt
     3421 gatgaaatga aagaatatga ctatgactta tacagaatag caagaaaagg aagatttggt
     3481 ataaatggaa ctaaggtatt accacagttt gaagttatgg agcatcaaga agttataaat
     3541 aaaatatcta atataccaca aaggtattat aaagttggat tcgactttgg atttgaaaaa
     3601 tcatataatg cacttttgag attagctata gaccatgaaa ataaatattt atatatttac
     3661 tgggagtatt ataagaatca aactacagat gatgtgactg ttaaagatat taaagaattt
     3721 aaagatactc aagaaccaat aaaagctgat gctgctgaac ctaaaactat aaaatatttt
     3781 agacaacaag gttttaatat atctggtgct aaaaaatatg ctggaagtag attacaaaac
     3841 actaagaagg ttaagagatt taaaaaaatt tattgttcaa gtgagtgtaa gcatacaata
     3901 aaggaattaa agaacttaac atatgctaca gataaaaacg gaaacttaat atatgatgaa
     3961 tttaatatag accctcatac atttagtgct atatggtatg gacttgatgg atatgaggta
     4021 tcagacatta aagagttaaa atatagtaat gatatttata acaaaggttt aggattgaag
     4081 aaaaataatc aatataataa gaaaggaggg actgtatttt agtgaatata aaaaatatct
     4141 tactcaattt agatgaaaaa gagttaagag atagaaagca tgcagaaaga gatttcttat
     4201 tttatttggg tgaatgtaga aataaaacta tgggattgtt agatgatgat tttttaggtc
     4261 aaagttggat tacttttgat aacctagact atacaccttc tcaaattgta gataataagg
     4321 taaaaccact tataaataaa caagctcgtt ttatgtttgg aaaagagcca actataatac
     4381 ttaaagcata tgaaaaagaa cataaagaag catgtgagga attaaggcag tatattgatt
     4441 caatattaaa tgccagcaag ttttggagca acacactaaa agcttttaag atagcaacta
     4501 taacgaaaag agtcttatta aggttagaag ctgaaccaaa tcaacctata agactctttt
     4561 atcattctat aaatgatttc aaatatcaag ttgatagtaa tgatattaca aaattaaagt
     4621 cagttgtatt tgttagattt gattcatcaa ctataaaaga agtaacagca aaacaaatat
     4681 ggtatagata tacttactat atgaaaaaaa gtgatgtcag taatcaagaa agttgcttta
     4741 taaagataga aaagttcaaa ggtgataact tatctaaacc tattaaaata acagaaaatg
     4801 ataccaagct ttctaaaata ccatgttggg tgattgttaa tgaacaaagc attacaaata
     4861 taagaggaat tagcgatatt gaggacttaa aacctttaca ggacacttat aataaaaggt
     4921 tgtcagattt taatgattct ttaaaatttc taatgtttgg tcaaacagtt gtagtagatg
     4981 cgacagaaga aacagttaat gcttgcaaga ttgctcctaa tgctttaatg gcattaaaaa
     5041 cacttgaaga aggttcagaa aaagcaaaac aagcacaagc gtatagggtt gagagtagtt
     5101 tttcaaatgc agaccctgta aactcttttt ttaaaagact tgaagatagt atgtatgaaa
     5161 aattagcaat acctagacct gaacaattac agaatatacc tagcgccaaa gctttgaaat
     5221 atttatatac agagcttatt gcaagatgtt cagaaaagtg gaatgattgg gaacctgcaa
     5281 taagaagcat gttaagatta atagttgaag cttgtagtaa atttaattgt tatgatgatt
     5341 ggaatcatga ctgggatgat ttaatgtttt ctattgtatt aaataaaaat tatccaattc
     5401 cagaagatga agaagattca aaaaggttgg ctcttgaaga agttaataat aatgttagaa
     5461 gtcatagaaa ttatataaaa gaatttggag atgatgaaga ttatgaggaa gcatttaatg
     5521 aagtactaga agataatgaa aagatacaat cagtagagca agaccagttc agaaaagatg
     5581 cagaaataga aattgataat attgatgaag aattaaatag taaatctaat aatagtgatg
     5641 agtaatgagg tgttgttatg aaggacaata cttatacaaa gaaagttctc gaagctagaa
     5701 aaaaactttt attattagat aaaaaagtac aattagagat attaagtgtc tataaagatg
     5761 ctagtaaaac tattttaagt gatattatta aaaataaaga gttgaatcta agtactaaat
     5821 acttaaaaaa gctaaataaa ttaatcgaga agtatatcaa tgagccaaat caaagattag
     5881 tacctgttat agaaaaaagt ataacagaag cttctaatat agctaaagat ttacaaatgt
     5941 attactatca atcaatagtt ccaaataaat ctataaatct tgcatgtgat gctatgtgta
     6001 taaagacaac tactaatgtt gtgggaaaaa tagttgcagg taacttttat aaagataaaa
     6061 ggtcattaga tagtaggatt tggggctata gtaataaaaa tagaaaagat attgatagat
     6121 taataaaagc taatattgca agaggtgcta atgcaaagac gttagcaaag agtttagata
     6181 attatgttaa tccagtcaaa aaaacagaag caaagacgtt agaagttgga atgaataaaa
     6241 gtatatctta tcaagcacaa agacttgcta ggacttctat aacacatgct tttgtagaaa
     6301 caagtgttca aaatgcaata aataatcctt tttgtgtggg attacaatgg aacctaagtt
     6361 ctcaacatta tattagacag gtgaaatgga gaggcgagga tgagtgtgat gagtatgcag
     6421 aacaaaatcg ttttgggcta ggtgaaggag ttttcccacc agaaaagtat cctattccac
     6481 accctaattg tctttgttat ccagttcaag tgattatacc aattagtgaa gcttctaaaa
     6541 tgatggagaa ctggttaggt ggtggagatg atgatattct tgatagttgg tatgatgaaa
     6601 taagagagaa aaataatagt gctccaatca ttaagaaaca aagaaataaa aagaataaaa
     6661 taactgcaaa tagcaaaggt gatagaatta aaaagaacat tactaattca atcaagaata
     6721 agtcaaagaa agcacctaaa cagattcaga aatatattaa taagtatgta aatccaaaca
     6781 gaataataat agataatagt caaaaaatac cttttgttta ttacactaag gttgatttag
     6841 ttggtattaa tccaaacatc agggaattta aatcttataa taaagaagct gctttacttc
     6901 atgagtttgc acatagaatt gatataaatg aaattaagag ctataataat attaagttcc
     6961 aacaagctat tgaaagtagc tctatgtatg ttataaagaa tattgagaac ttgcaaagta
     7021 tatatactaa ttctaatgag ctgtacaata atgaatttat tagtgacatg ttaggagcat
     7081 tatccaataa tgagtttgag gatttattag caactcatag tgaaaagtat tggagtaaaa
     7141 acagaaataa agagaaagaa atatttgcta atttatttac actaaattat cagaataata
     7201 aagaaataaa cagttttatt aaagaacatt taaatagttt agataaaata tttaatgaat
     7261 tgctaggagg gatttagatt gcttgaagaa atgagaaaag ataaaaagct gttagagttg
     7321 agacgattat ataaagaaaa atatggtaaa aatgcacctg gatttaacta tgatgaatac
     7381 aatagttatg ctgagtacaa agaaaagcta aaagaattga tacaaaagta aagtaagcac
     7441 ttattaatta aaggttaatg agtgctttta ttatgcttaa ttttaaggag gaaattgaat
     7501 gttagagtat tttaaaaagt tacttggaga tgaagaagga caaaaagttt atgaaaaatt
     7561 atctaaagat aaagaaaata agcttctttt agataatatc aaaagtccta gatacgttga
     7621 aaagacagaa ctagaaaatg ctaataaaga gattaaagag tataaaaagc aaatggggga
     7681 tagagataag caactaaatg atttacaagg caaagttaaa gataacaaag agttatcaga
     7741 tgaaattgaa agtcttaaaa atgcaaataa agaaattaga gaaagtgcag aaaaagaaat
     7801 tgaggtttta aagtttaata cagcttttga aagagttatt gaaagttata atcctagaaa
     7861 ctcaaaagct ttggctgctt taataaataa ggataatatt agttttgtaa atggtaaatt
     7921 tattggatta gatgaacaaa taaaagctta tcaacaaagc gattcttatt tattcaataa
     7981 tgaaaaaaac aaaggagatg atgaaattgg aggaactgga ggattagaag gtggaacaac
     8041 ttcattactt gataatgata gcgaaataag tagtataggt gagttgttag ctaactctaa
     8101 agtaaaaagc gaaaatatag aagctcaaaa gaaatttttt ggagaagaat aggaggaaaa
     8161 tatatgagta tagaaaaatc cgagatttac atgggggaaa ataaaactac attaaaattt
     8221 gcaggaaact tatttcaaaa tgtaaatatt aaagtgaaaa aaactgatgt agctatagta
     8281 gatggaaaga gaattttaaa agcgggtaca ttaatatcaa aagatggaaa attagtagat
     8341 ggtacaactg ttacaaatga taaagctttt ggtctagtat atagaaatat agattttact
     8401 tattcaaatg gtaatgaaag cattccagta ctaatatttg gatttgtgga tgaaaagacc
     8461 ttaccatcag ttataccaga agaagcaaaa caagcaatga aaatgattat gtttttataa
     8521 aataaatatt aggaggaatt aaaatggatt ggaaagactt tatagactct aaggagatag
     8581 ctaaatatat aaaaaaatta ccgctagaaa tgttaatagg cgaatctttg tttccaagaa
     8641 aaaagcaaat aggtatggac ctaaaatata ttaagggagc gaagaaaaaa ccagtagtgt
     8701 taaaacaaag tacttttgat gttgctgtaa aaataagagc attaaaagct caaatagaag
     8761 ttaaatcaaa gagaatgcca ttttttaaag agagtgttct tgtaaatgag gaagatagac
     8821 aacagttact tttagcttct aaagctcaaa acaaagaact attattaatg ataatttcac
     8881 aaatatatga taattattta gctcttgtag atggtggcga tatgcaaatg gaaagaatga
     8941 gaatgcaggc tctagctgat ggagtaataa atatcgtatc agaagatgga gatttagtat
     9001 ttgactttga agttccaagc aatcataaag aagtgttaac tggaagtgca acatgggata
     9061 atccagatgc agatattata ggagatattc aaagatggat gagaataatg agagatgaag
     9121 ggaatccgtt acctaagaga atggtaatga caagtaaaac ctttggatat tttgctaaaa
     9181 ataaggctat taaattagat atagatagag acggtagagt tattttaact gatgaaatga
     9241 ttaaaaatta tcttaaaaat aaagttggtt tatctgttgc catagtaagt ggaacatata
     9301 aattagaaga tgaaagtgag gaatcttatt tccctgataa caaaataact tttattccag
     9361 atggagattt aggaaaaact tattatggta caacaccaga agaagctgac aaagtatatg
     9421 gttctaaatt agattgttct gttgttagaa ctggtattgc cataacaaca atgagattaa
     9481 ttgacccagt aacagttcaa actaaggtat cacaactagg tatgccaagc tttgaacgtg
     9541 ctgatgagtg tttctttgct acagtagcgt aattaaggag tgataattat ggctaaaaag
     9601 aaagagaatt taatgcaagt aaaagcttta gtttacttaa aatatgataa tgactgctac
     9661 aaaatagatg atatatttga gattagaaaa actgaacaag aacttatgga agaaaaagga
     9721 tatatagaag ttataggaga agttgaagaa aagaataata atgagcttct taataaagat
     9781 ggtgagtaga tatgtctatt actgatttag ataaactaaa actcaatttg caagaagaag
     9841 agtatcctta ttttacagat gaacaacttg tgatgttatt agaatctaat gagaataatg
     9901 ttttaaaagc ttcatggaga ggttgtttat taaaaggtgc tacagatgat gaaataaaaa
     9961 taggtcctat tgaaactaag tctagcaaca gctcatattg gttgactcta gcagatatat
    10021 ataaaactga ctatttagaa gaaaaatcaa agaatgaaac aactaacaca ggatataaaa
    10081 catctatgac aagagctgat ggacaatgag aaagttaaga gctgataaga taataaagac
    10141 tatcaataga ggaatagctt taaatcctca aacaataact atagagcagg aaattaaaca
    10201 aatagtggat ggagctattg atattacaaa tgaagtaaaa gaactaacag tagttatata
    10261 tcccgaaaaa actaatgata cagtaataag tagtgaaact attggtacag cttataaaaa
    10321 taaaaacttt ggtatggttg cagataaaga agctgattta agattgaata ctgaaagtga
    10381 tataacattt aaatgtattg aaggtactat gaaattaaat tatgtaaata ctgttgtagt
    10441 tgaagaacaa gtttgtggtt atatatgtgg tcttaaaaag ctagattaga ggtgatttat
    10501 tatgagcgtg tttactaagg ctataaatga aattgatagg aaaaaggcta caatgccact
    10561 tttgtgtatg aatatagctt ttatgttaga aggagaagct aaaaatagtg caaagtggac
    10621 tgataggaca ggaaatgcaa ggcaaggtat aacaggaact agcttaggtg gaggaaatca
    10681 atacattgta cgtctaggtc atggggttga ctatggaact gttttagagg aaggttcagc
    10741 acctcatatt ataaaaccaa agaatgctaa agctttgttt tggaatggag cttcacatcc
    10801 tgttatgaag gtacaacatt ctggcactaa aggtacacgt tctttagagt ctacagttag
    10861 caagaatata tctaaaatag gtaaagcatt agaaaaacat tggagtgatt aaaattgaaa
    10921 gataatttag atgatatgaa attaacaaat gttactcttg attattgtga agaaaaaaga
    10981 ataggaaagc ttattattga tggtgtagac ttctctaata aggctacttc tgtacattta
    11041 gattttgagg ggtgcagtat accaataata aaagtggaac ttgtttgtga cgaaataaaa
    11101 ttaaatggtg actttatagt aaaaaaagat attgataagt ctatagaaaa gaaagtaatt
    11161 agtgaaatgt ttaatcgttg gagtaaataa atgagagcag gaataagaaa agccttaatt
    11221 gagaacatac caagaattaa agattgttat gaaccaactg tccctaataa aaagactata
    11281 aaaccttata ttgtaattgt ccaaggtgaa gatgctgaca atgaaggcaa tgttataggt
    11341 tttagaagga ctataaatat ttggttatat gagaaaagga ctacatttaa taaactagat
    11401 gaacttacaa aagaagtcat agaaacctta gattttaaaa ctataataga tgatacttct
    11461 aacgaggtat ttacttgtat ttatgagggt gcagttggtc aagatgttat agatgaagaa
    11521 tgggaagcta taataaggtg tctgagattt agtgtaatag ctttagatga taaagaggat
    11581 gcaactagtg atagatgggt agaagcttta tcaaagtaca caaaagattt attagaaatt
    11641 gagagttata aagataattg gaagaaaaat tttatagctc catgtgcatt atggagaaca
    11701 acaaatgtta aaaacaaaag gattaactat catctgattg agattactaa aactatgaag
    11761 tgtcatgttg taagtaaaaa taaagatgaa atagttaagc ttcttgaaac attagaaaca
    11821 cacttaataa tcgataaaag agtaagactt agagaagata agaatatgta cttaactctt
    11881 gttagcgtgg ttgaggatag agaatcagat atgttttcta gtggtcaatt aactgtagta
    11941 tttaaaatga taggtaagat aaaaagagaa ggacctatta tgaatgagat ttataatagt
    12001 ggaaatttaa gataaaatag gaggaatagt attggctgaa acatcaaata aaaagactgt
    12061 aagtaagcag gaagaaaaat attcaaaaga agatttctta aaaaatagtg aagcacttgg
    12121 ctacgaaaaa atggtagttg caggtgcttt atttaattgt aagaaaaagg aactcacaaa
    12181 agtagaattt gagaaagcaa taaaagactt tctagaaaga gaggtgaagt aaaatggcaa
    12241 ctggtacatg gaatgaaaaa gaaaaaaagg agatacctgg tttttataac agatttaaaa
    12301 cacaagcaga aaaatctaca aacacaggat taaagggtag attagcaata cctattaggg
    12361 ctaattgggg agatgttggt aaggttgtaa caataaaaaa tgatttgaga cagcttaaaa
    12421 cttcgtttgg tgatgatatg aactattcag cgtataagtt aggtaaatta gctttattag
    12481 ggaatgtaaa agagctgtta ttatataggc ttgtagatgg aaatcaaaag aagggtacat
    12541 taacactaaa agatactaca gagaatagtg caaaagatgt aattaagcta gaaactaagt
    12601 atccaacagc tagaaacttt aatgtaacaa taaaatctaa tttagtagat tcagataaaa
    12661 aggattttat tttctttgaa gggactaaac aactattttc tagctcagtt aaaggtacta
    12721 tagatgaaat agtactagaa ataaattcta atttagataa tgaatatgtg gttgcaacta
    12781 aagtagctga tagcgacaca acattagcga atgtagtaaa tgtagcactt gagggtggta
    12841 atgatggttg cacatctatt actaatgagt cttatctaaa agctttagaa gaatttgaaa
    12901 gatatagttt tgattctttt gtacttgatg gtgtagctga ggaagcattg caggaaacta
    12961 caaaagcttg ggtagctaag aataaggagc taggaaaaga tatattatta tttctaggtg
    13021 ggaaaaaaga ggataatata aaacagataa atgataaatc aaaaggtttc aatgatgaaa
    13081 atatagttaa cattggaagt tcagcttatt atgagggaat aaaatataca cctagtgaag
    13141 tagctgttta tataggagca ttagcagtaa gcaaaggtat aacaggaagt atatgtaatg
    13201 ccaagactat atttgaagaa gtagaaccaa gattaagcca atcagaagtt aaagagtgtt
    13261 tgaaaagtgg tactttaatc ttagactttg atgatggaga tgtgattata gttgatgatg
    13321 taaacacatt taaaaagtat gtagacgata aaaacgaagc aatgggatat atctctaata
    13381 tcatgtttat taacactata aataaagata cttcattaaa aagaaaagag tttgtaggta
    13441 agatattcaa cgattcgaca ggtcaaacaa ctgttatatg cgcattgaag aaatattttg
    13501 aagaattgat gagtcaaggt attatatctg aatttaatgt tgatatagat acagaacttc
    13561 aagcaactgc taaagcagat gagttttatt ggaagtggga tgctgtgaaa gttgatgtca
    13621 tgaaaaaaat atatggtact ggatacttag gataaaggag gttatagatt atgtataatg
    13681 atgattatat agaagaagcc agttttctga atggttcaga tgtagttata cttattgatg
    13741 gtgtagaaga gctatacatg gaagaaataa aagctgattt tgagcaagat gagcaaagta
    13801 ttaaactgtt agggtgtcaa aatgaaatat caagggttgg tactactaaa ggttcattct
    13861 ccttgaatgg atataagaca gattcaaaat ttgcaaaatt aggatttagg tcttttgaaa
    13921 taatatataa tttatctaat tctgaaacat taggatatga aagtattaga ttaaagaatt
    13981 gtagattaaa aaaattgcct cttataaatt ctaaagctgg tgaaattgta aaaatagaag
    14041 tagagggaag ttttagagga tatgatttgt taaatgaact ttaaaacaaa ataatgttgt
    14101 actttaaagc tatgaacaat taattttgtt tgtagctttt taaaattaaa aatattggag
    14161 gaatagtatg tcagagatat ataaaagaga gttagaaaat gaagtagtag aagaagataa
    14221 tgatttagat gaagaaatag aagaaacaaa tgaagataga ttgaagatga aagaggatga
    14281 aataatagca aagctattgg aggattcacc agttcctcag agaactgtgt ttttagatag
    14341 attaggaata ccaattactt tgaaagcatt aacagaaaaa gagattagta aaataagaaa
    14401 agaatgcact aaaattgtaa aggtccaagg gcgaagagaa gaaaaattaa atgatgatga
    14461 gtttactcta gcattaatag aaaaaggaac tgtaaaacct aatttttcta atcaaaaatt
    14521 acttgatgct atgaaagtaa caaatgctag agaatttata aagagaaaat tcttagctgg
    14581 agaattaagt aaaataagtg accaaatatt agaattatcc ggtttttatg atgaaataag
    14641 tgatgatgat ataaaaaact aataaaaatg gggggacgac tgactgtttt aaataacatc
    14701 tttgttaagc atcatgttcc cccagatatt tatgttaaga aaaatttaat gtctcaacgc
    14761 ttaatgaaag tatttactca aaatgaaata gagcaagaga ataaagctat gaaaaaataa
    14821 atatctacag aaaggtaggt gagagaaata gctaaaaagg aaatgtatca tattgatgtt
    14881 gtcataagtg caaaaggcga cggagaaaca aaaagcaaac taagtgctat ggaaaaatac
    14941 atgaagcaga cagaaaagag aatgagagca ctaaatagga taaaagctaa tccagttata
    15001 caagctcaag ataaaacatc tagtgttgta aatagaatta gcaacaactt aaaaagagtg
    15061 ggtagaacta tatctacaac cataaacgca aaagatagag catctagcgt tgtaaataga
    15121 gttaaaaaca aagtaaatag cttacttaca agtcgacaaa gagaagtttt attaaaggct
    15181 agagacaaag ctagtcaagt tgtagataaa gtaaaagcta aggtacaaaa tttgactgcg
    15241 gctacaataa ttagcttgaa tatgaaagct gacccagcac taagagttat ttctcaaact
    15301 agaagtaagt taggagaact caaaaacaac acaataataa atattaaagc aaaaggtgaa
    15361 gaagcattaa atactatttc tcgtactaag agtaaattac aagaatttgt gagtaagaga
    15421 tatgaagcag cagttaagat tagagatgaa gctagttcag ctttaggagg gttaacagga
    15481 aaaataaact cttttgtaag tggagcaata agtaaattcg ctcaattaac aacagcaatt
    15541 actgttgcac ttggaggtat aggggttggt tcttcgataa aaacttttgc taatttcgag
    15601 caaggaatga aaaacgttca agctgtcaca ggagcaagtg gaaaagaaat ggaagcttta
    15661 actgtaaaag ctagaaatct tgggaaaaca actgcatatt cagctagaga agtgagcgat
    15721 gccatgtatt atgcgggtat ggcaggttgg aaaactaatc agattatcga aggtatgcca
    15781 ggcatcttga atttagcagc aacaggcgga acagatttgg cactaacaag tgatatagta
    15841 acggatggct taacatcatt gggaatggct gctaaagata ctaatgaatt tgttgatatt
    15901 atggctgcaa ctataacaaa ttctaataca aatgttgagt tatttgggga aacacttaaa
    15961 tttattgggt ctctaggagg ttctttgggc gttcctatgc aagatatagc tttagcgact
    16021 ggattaatgg cgagtagtgc tatcaaagga agtcaagcag gaacttctct aagaatgggt
    16081 cttttaagac ttataaatga acctaaacga gctgcaacag ctatgagaaa atatggaata
    16141 gaaatgaaaa caactaaaag tggaagttta gatttagctg caacaataga tgaacttaga
    16201 aataagttag gtaagcttag tgacacgaag aaagtaggag ccctaggaga tatagtagga
    16261 gctaatgctt caagtggttg ggctgcaata gtaaatgcta gtgaaagtga ctacaataaa
    16321 ctaaaaaaag ctattacaga aagcgaggaa gaagctaaaa gaatagctga catgaagatg
    16381 gattccttga atgggcaatt cgctaaatta aaaaacacta taaatgatgt aaaaattagt
    16441 ataggagaaa aattaggtcc agtaacaaga aactttatgg aaaatataat tagtagtatg
    16501 cctaaagttg gtgattcaat tgtaaacttt gtgagtaatt tcattaaaaa ttttgataaa
    16561 attaaaagtg ttttacaagg cgtaatttct attattggtg gtgttgttgc tgggtttatg
    16621 gcttttaaag ctttaacagt aatttctagc gtaataagta taattacaaa gatagctaca
    16681 gcatcaacgc taattggagg aatagttgga gttcttggac ccgttggtgc aatagttaca
    16741 gcaacaggcg tattagcagg agcattttta tttgcttatc aaaaatctga aacttttagg
    16801 tatggtatta aaaacatagg taaatcaata tctaattttt taaaaccttt aaatcctttt
    16861 atagaacaaa taaaaggtaa atttaaagaa cttataaatg cgttaaaacc tttgtttgca
    16921 gttttttcta aattaggctc aatgattgta agtgctttat caccagttat acaatttttg
    16981 gcgacagttt tcataataaa atttatgtat ttatttaatg taattgttaa caaggtaaaa
    17041 gctatagtaa atacaacaac agtagtagta aatggtttaa tagagatatt taaagggata
    17101 gctgatgtag ttaaaggttt tataaataga gatatgagcc aagtagctaa tgggcttaaa
    17161 tctattttta aagggattat agatattgtt aagtcattgt ggagtgggtt agtagatttt
    17221 gtgacatcgc caattcaagc tgtagtcgat attttggata ctaagtttgg caaaaaagtg
    17281 gaaggcataa agaaaaaatg gaatgaatta aaagactttt taaaaaatcc tactaaagca
    17341 gctccaaaag ttcagcctgt taatttatct agcgagaaag catcaagcga actgcaaact
    17401 tcatcaaacg gagcaaaagc atatataagt tcgttaggtc aaaaaatagg tgaaggtatt
    17461 ggaaaaatta aagataaatt tggagaactc aaaatatctg caacagaagt atttaataat
    17521 atagtatctt tcgtaggagg caaagcaacc gaattaaaag ataaactttt agaaggcata
    17581 aaacctgcta tagatacatt taaacaatca ttaagtaatt taaaagaagt ttttggagat
    17641 tctttagata gcataaaaga agcttttgga ggtttaaaaa ctgtatttga tgaaaatatt
    17701 aaaacgcctt tcgaaaattt aaaacaaaaa gttttagaaa caaaagaaag tttaaaacca
    17761 gtttttgata acttaaaatc tagctttgca gaactaggaa aagctcttga accaataaaa
    17821 gaagcattta gtggaataaa agatttcttt tcaaatttgt ttaagccaat taaagatgat
    17881 ggagcaacta agacaactaa aaccaatatg gatgagttaa aacaatcaac acaacgtgtt
    17941 ggaacatctt tcaaagagtt aggaaatgct ttcaaccaat taaaagaagc agcaaaacct
    18001 tttatagact atttaaaaca gataaaagat tctttaacat ctactctggg agatataggg
    18061 ggaggattac tcaaaggtgt agcaacttct atagttttag ttataacttc tgttattaat
    18121 gcaattgcat ctattataaa tgctgtagca ggtgctataa aaggtgtaat tgatataata
    18181 aaaggaatat tcgaaatcat aggtgggata attagtggtg atggtgaaaa aataaaacaa
    18241 ggattttctg atgttttcaa aggaattggg gaagtagtca aatcattgtg ggaaggtata
    18301 aaaggagttt taggagcacc acttaaagca gttgtaaatt ttattgataa tggattttca
    18361 gaaaaagtag ggcaagtaaa acaatggtgg tctgatttaa aaactaatgt aggccaaaaa
    18421 ataagcggat ttgttagttt tgtaagcaat ggttttcaac aaaaagttca acaagttgga
    18481 atgtggtggc aaggacttaa agtaaattta tctggcaaaa taagtggatt tgtaagtctt
    18541 gtagaaaacg gatttaaaag taaagtggat tcaattaaat cagcatggga tagtttaaga
    18601 aaaaaattat cacaaaaaat aactggtttt gtaagtatag taaaaactgg aataagtaat
    18661 gtattggacc gttttgctga tggtggagtt gcaagtaaac caagtatttg tggagaagca
    18721 ggtcctgaaa tggttattcc tctttctaat agtaagagaa gtagagcatt aagtttatat
    18781 gaacaagcag gacagatgct tggaactaaa gcaagcaata atgttattcc aatatctcaa
    18841 aaattaggaa ctagttttaa ttctgcaagt agtatccaaa atagtaattc tagtattatt
    18901 aataatgtta gacaatttcc tcttacaaat gctttggata cagataataa agaatataaa
    18961 gaagctacac caaataatgt taattcttct aatactataa atttaggcgg aatatctata
    19021 aacattcaag agagtaataa caaggaagat atgatacaag aaatattatc tcaagtagaa
    19081 agtgaaataa gagaagcatt acaagacatt ggataatgtc gaattgttgt taaaaaaatc
    19141 ctccttatag atgttataat aacattatag actattgatt tatgagggga ttaaaatgaa
    19201 tttttttaaa cgaaaagatg acataaagaa ttttaagctt atgtatataa aagggcaccc
    19261 acattttaac aatgaactta catgtactat gtatatagat aaaaaactag tatctgtata
    19321 tacattgcaa caagtaccta ttagtgaaat aaaactttta gatatagaga aagtctttat
    19381 tgataatgat atgctaatta tagaaagcaa aaaaagtgta ttgtatttta aaagtaactc
    19441 aaaagataaa atatgcaata taattaatca aaatatagca gatatgcaag ataaaaagac
    19501 agatactata ttaagaaata aaaataaaat agttaaacaa ggggtaatga agatggatta
    19561 ctataattct gttggagttc ctaattgtag gggtgaagca ataagtataa ttattgacaa
    19621 ccataatgca tgtttagtta ttgagagcaa acttttaaaa gaagaaaaaa tgcgtattaa
    19681 attttctgat ttatatagtg taaataagta tagtgagaaa gaaatagcat ataataataa
    19741 gtctgttgtt ggaagagcag ctataggaac tttatttggt cctatgggaa caattgttgg
    19801 tgggttgtca ggaactggaa ataaaaaaag taaaactgaa aagttgttta taagtattga
    19861 atatttttct catggagaat taagggaaat aatagctgaa gattcaatac ttaattttca
    19921 tagccaagaa tttattgata atataaggag aaaaataaat ataagtaaga ataatttaga
    19981 aaataataaa gaaaaacaaa taaaatcaaa tgatgatgca atacaacaag tgaaagggtt
    20041 aaaagagtta ttagacatgg gagcaataac agaggaagaa tttaataaga agaaaaaaga
    20101 attattgaat ttataacaag agaaaataaa taagtataaa catacaaagc acttgaatat
    20161 tctgttgttt caagtgcttt atatgttaaa aaatggtata atataggtaa ggctttagca
    20221 gtgttcgatt ttttgaaata ttgcttgtag tgattgaaat tactatatta caggtatgct
    20281 ttatgaaatg ccaaaaaaca ctactggtcg ctcactgcaa atttaagaaa gctatatata
    20341 tgtaagtatt ggaaatacta aatttatttt gggattttat attaactatg tggtatgtaa
    20401 atacggcttt cagttgttac taacgcattc tcaatatgca ttttatatta actatgtggt
    20461 atgtaaatac tactttttga gaaggagttg aacttaaacc taacaatttt atattaacta
    20521 tgtggtatgt aaataacaga agaaacaatt tttattcctc cttcaaatac aattttatat
    20581 taactatgtg gtatgtaaat aaggctttag agactgtaag aagtatattt gctcaagatt
    20641 ttatattaac tatgtggtat gtaaatgcta aaacattact tgataaaaac aaaattacag
    20701 gattttatat taactgagtg gtatgtaaat ttatcaatct tagtattaga tgtatctttg
    20761 tataattgtt ttatattaac taagtggatt caaaactaaa taaacaaaga aagcacttac
    20821 ttttggtagg tgcttttgtt ttgctcaaat tggtcggttg agtaaaataa ttagaaaaaa
    20881 ttgggataag ttattgactt ttgtctgccg aaactatata atataattaa ggcagacaaa
    20941 agtgaggtga agatatgcca agtaaaaaaa taggtagacc tacagataat cctaaaggca
    21001 gtagaataac tataagatta gatgaagaat ctaaaaatat tctagagaaa tactgtgaaa
    21061 aagaagatat agacaaagcc gaaggagtta gaagaggtat aaaactacta aaagacaaat
    21121 aaaaagtagc ccaaccgccg accaaagcaa aatggactac ttaacctaga gttatctcta
    21181 tatgaaatat tctatcatgt aaagataact ctttcaagat aataaatcga aagggtgatt
    21241 tttgtatgaa taatgaactg atgaattttg aaaataatga attaggaata aaaataagaa
    21301 ctattaaata tgaagatgga agtataggaa ttaatgcaga ggatacagct gtagggtttg
    21361 gatggtgtca gacacagaat aaaaatggta agcaatatgt atcaattaga tgggaaacta
    21421 ttaataaata ctgtaaagaa tttggtttcc tcaacttgtt ggggaaagat gagtatatac
    21481 ctgaatcatt attttatctt ttaggtatga aagctaaaaa tgaaatagca gtaaaatttc
    21541 aaacatggtt agcagtagat gtactaccat caataagaca aactggtgca tacataacta
    21601 acaatgctaa tcccgaaaaa ctaagagaaa aagcaagtga gattgaaaag ttacaattag
    21661 cctataacag cacatctatg ttaaaagaat tgctagatgg tgcaggattt gacaataaat
    21721 ccaaactatt aacagctaaa acattatata aaaaggcagg cattgattta cctatagaaa
    21781 ttgaagaaga ggaatctttc tttgacacaa aacaaatagc atctaaactg aaaatatatt
    21841 ctaagagtaa taaaccagca caacttgcta tttgtgagat tattaaaaag ataaaattag
    21901 aagaaaacga agttaaggga gtttgggaaa ctaatggttc ttggactggt actgtaaata
    21961 aatatacaaa gagtgtaata gataaggtta gaaattggat agaggaaaat aatagaccta
    22021 ctaagattgc aggtgagaag aagaattatc atgtggttta taaaatcgag taaatttatc
    22081 agttgtatta aatattttag tttagttttg agggggatta atacaatgta tgagaattta
    22141 cttgatgatt acaatctaaa aactgataaa gagagaaaag attttataaa gtttgctata
    22201 ctattatata agttggaaca gaatgataaa gagaagtttt atggatatgc agagatattg
    22261 aaagaaattc ttagagaaca acaagagaga gaaaataatt aaataataaa tagataaagc
    22321 acttataagt acataaaagt gctttatctt ccaaaatatg ctataatatt attaacttgt
    22381 agaataagga tggttgctat gattgcagtt aaaaaactaa aattaactat agttgaagaa
    22441 gaagaaaaaa gaaaagaaca atataaattt ataagagata gccaatatgc acaatatcaa
    22501 ggacttaatc tagcaatggg aatattaaca agtgcatatc tagcaagtgg cagagatata
    22561 aaatcagact tatttaaaga ttctcaaaaa agtttaacca actcaaatga gatatttaat
    22621 ggaataaact ttggtaaagg tattgatact aaaagttcta ttacccaaaa agttaaaaag
    22681 gatttttcta catcattaaa aaatggtctt gctaaaggag agaggggttt tactaattat
    22741 aaaagagatt ttcctttaat gactagaggt agagatttaa agttctatga agaagataag
    22801 gagttttata taaagtgggt aaataaaata gtttttaaga ttctaattgg tagaaaagat
    22861 aaaaacaaag ttgaattaat acatacttta aataaagtcc taaataaaga atataaagta
    22921 agccaaagtt cgttacaatt tgataaaaat aataagctaa tacttaatct tacaatagat
    22981 ataccatata agcaagtaga tgaaatagta aaaggtagag tttgtggagt agatatgggt
    23041 atagccattc cagtatatgt agctttaaat gatgtttcat atgtacgaga aggaatggga
    23101 actatagatg aatttatgaa acaaagactt caattccaat caaggagaag aaggcttcaa
    23161 cagcagctta aaaatgtaaa tggtggtaaa ggtagaaaag ataaattaaa gggattagaa
    23221 tctttaagag aaaaagaaaa aagttgggtt aagacctaca atcacgcatt aagtaaaaga
    23281 gtagttgaat ttgctaaaaa aaataaatgt gagtatatac atttagaaaa attaactaaa
    23341 gatggatttg gagatagact tcttagaaat tggtcttact atgaacttca agaaatgata
    23401 aaatataaag cagatagagt tgggataaaa gttaaacatg taaatcctgc ttatacttct
    23461 caaacatgtt cagagtgtgg tcatgtagac aaagaaaaca gggaaacaca ggctaaattt
    23521 aaatgtttag aatgtggatt tgaagctaat gcagactata atgctgctag aaatatagca
    23581 aaaagtgata aatttgtaaa ataaaataca ataatatatg tacgggtgat ttagtgtaga
    23641 tggtaatatc tccaagataa aacttgcatc cgaagggtga gggtatagat aaaacgcata
    23701 aggtagtatg ccaaatatgt gctataacca ctcactaagc cgaaaaaacc ctagttataa
    23761 tggcaactag gcacactatt aatatataaa atgcagtgag cgaactttta catttacata
    23821 ctttaagtag ttgaaattac tagggtgtaa caattatttt tgaaatgtca aaaaacacta
    23881 ctggtggttc actgcaatat tttgtaaatt acatagacaa aatcattgca attactgtgt
    23941 tatatttggg gttttataat gactatgtag tgtgtaaacg tactccattt actacaccat
    24001 tagtcaaatc tgtaccgttt tataatgact atgtagtgtg taaactaaaa aatacaaaga
    24061 agaattgtac aacgaatttt cgttttataa tgactatgta gtgtgtaaac aaatacttca
    24121 ccaaataaac ccctagtttc tgtatctagt tttataatga ctatgtagtg tgtaaacgat
    24181 gaacaataaa acaatcatct aaagacgaag aaagttttat aatgactatg tagtgtgtaa
    24241 acttagtatc tgctacaaag tattctaaag tccatttggt tttataatga ctatgtagtg
    24301 tgtaaactct tgattaaatc cgattttaaa tgaattaaat gtagttttat aatgactatg
    24361 tgtatttaaa ttcaatattt tgaaagtatt ataataatca aaaataaata ttaaaacaaa
    24421 actaacactt actcaggtag gtgttttttt gttgaaagaa ggtgattatg atataaaaag
    24481 taataaatag gtaaaatatg taagaattat atgatataat aattgtagca aggaaaataa
    24541 taattgaaaa gtgctaagag tggttatttc catatttaaa cgccaaattc catagcggaa
    24601 ggaggtggaa cgatatggtg atagattttt tattgagtat actagctggt gttatatcag
    24661 ctttcatgta tgagaaaata aaaaaccact caaacgccaa taagagtggt ttaaaaaaat
    24721 aagttatttt taatctgatt ggaaatagct actcttgtat aaagtaaatt atttccttgc
    24781 ttttattata ccacaaatta gaaaaaatat tgcatataat attttttata gtcaataaaa
    24841 agatgaaatt ttttatacaa aataataatg aatctaaatt aatgaataaa tttaattgta
    24901 aaacggtaaa tacagtaagc acttacaaac atgtaggtgt ttttttatac aaaaaatcaa
    24961 aggaagtgaa tatatggtaa gaagactgcg gtgtaatgtt aaaaaatact ttagaaagga
    25021 agtgataact tggtaataga tatttatcta aaaaatgaaa aagaaaaaat agatttccat
    25081 tttccagtaa ctccgcttga ttctttatct attaaaaaag aaaaaaggtt tgaaactgta
    25141 gatatagtaa acttaggcga atttgacatt aaaaaagaag gagagaagat aagagaaata
    25201 tcatttaaaa catttctgcc taacttatat gatgcttctt attgcagata tagtgagtta
    25261 aaaaatccaa tcgaagtagt ggcaatgctt gaaaaatggg tagaccaagc cgaaccttta
    25321 agacttatta tgacaggctt tggctacaat ggattagtta caatatctag ttttagcaat
    25381 actcaaacag caggaagaga agaagataga gacattgaga taacatttag aacttacaga
    25441 gaactgaaga tagagacatt aaaaaaagaa actaaaagta atactaaaac agatttaaaa
    25501 gataatagac ctaataccca aactaaatct aaaatatata ctgttaaagc aagtgataca
    25561 ttatataaga tagctaaaaa tcttttaggt aagggttcaa ggtggccaga gatttacaat
    25621 atacccgaaa ataaaaaagt cattggtaaa aatcctaatg tgattaaaaa agggcaaaag
    25681 ttggtgatac cttctaaatg aaaataatat taaatggaaa atatgatatt gcaaatttta
    25741 acgagggaat aacattaagt gaagctatag acggagttgc atacaagatg gatgtatctt
    25801 taatagaacc taaacaactt aaagatataa atattaaaaa aggtgataaa atagttctga
    25861 ttgatatagc atatgagagt aaaaaagaag aaacaatctt tgatggagtc atatgggaaa
    25921 ctaggagaag tgaaaagagt aagaaactga cactatcttg tagagaaaga acagtttaca
    25981 tggaagaaag tgaagaacaa tactcgttta aagaaaacac agcaacacag aggattgagt
    26041 actactgtaa gcaatggaat ataccttact acaatctagc taatacaggg aagaaacttg
    26101 ctaaagtaat acataagact aatatactag atatgattaa aaaggactta aaagaaacag
    26161 caagtaaggg tggagactta tttagagtaa gaatggataa taagctgaaa ttatttaagc
    26221 ttggcactaa tgcaaatgta tataaattag atagcatatt agaagatgca aattttacaa
    26281 gtagttttaa tgatgcagta acctctgtaa aagttttagg taagagtaaa gatgaaaata
    26341 caaaagcacc tataattggg acatataaga aggactcaga taagtatgga acactacaaa
    26401 agattaaaca agatgaaaag ataaaaaatg ctaaagaagc taagaaagca gcagaagcaa
    26461 tgttcaatag tggagaggaa acaataagtg tagattgtgc agtagatata aatagaataa
    26521 gagcaggtga caaggtaagt ttaaaaaata aagaatatta tgttatagat gtcactcata
    26581 cactagattc tagaccgaaa atgaagctca atatagggac tttagaatat ataaggagga
    26641 agttttatac aaatgactga tgctagattt aatggaattg ctagaatatt gaaagaaaat
    26701 atgaataaaa gtgtagcaaa ttgcactttt ggaatgggtt gtgaacttgc agaaataaca
    26761 acaaatggat taaaagtaaa tggttataaa gatgaaatac aggattatct agtattagag
    26821 agtttaatat taaaagaaga ttattttact ttttcagatg aagctttagg tggagaatat
    26881 agacataagc ataaattaga aactccaaaa gagttgaaac cactaaccat aggtgataag
    26941 gtgctagtag ctgtcatggg ggctgaattt gtagtaattg ggagggttgt aaatgccaaa
    27001 cctatttcct caaagtgaaa cttttgaaac tgtagaatta aaaaataata atgagaatga
    27061 actggaccta aagggttctt ttttatttga ctttataaaa ggtgaatttg ttaaaaatgc
    27121 agatggaaca ttaaaaaaat gcgataaagt tcaagcatac aaacaatggt gtcagaaagc
    27181 tatattaaca cctaggcaca aaaggtcagc ttactctaat atatatggaa gtgaaataaa
    27241 agacttaata gctagtaact tatctcaaag tgcaaaagag cttgaaataa ctagattaat
    27301 aaaagaaact attttggttc atccttacac aaaagaagta ggagagttta gttttaattg
    27361 gctggagaat agcaggttag tagagtgtga atttaatgta ttgacaatag atgatgaaaa
    27421 tatagtaatt gatggcaata taaaaaggta ggtgattata tggaaagaga gctacccata
    27481 ccagtatttt taacagaaga tgaggactct gtacatgaaa ggatgttaag caactttcaa
    27541 gatgtttcta cattagaagg tgactttatc tatgatgcaa caagacctac agcagagcaa
    27601 atagccgaat taaaacaact aggattacaa aataatttaa agatagcatt tcctcaaact
    27661 agctatggaa cttacttaga gtggcttggt gaatgtaagg gagtatttaa aaatcaacct
    27721 acaaaagcaa taggaacaat tacatttaca ggtgtacaag gaactataat tacaaaagga
    27781 actattgtta caactatagc tacagatgaa aaacagagca tagaatttga gcttcttgaa
    27841 actaaaacta taggagcaaa tgaaaaagta gatattaaag cagaatgtag gattgcagga
    27901 actataggaa atgtatctaa taacactata actgttttac taggttctat tagtggcatt
    27961 aaatctgtta ctaataaaga agatttaaaa ggtgggacag atatagaaga tgaagaacat
    28021 tttagagaaa gagttcttgt agcagagcaa gaagacaaac ttagtggagc tagttcagat
    28081 tatattcgtt gggctaaaga agttgatgga gttggatatg cttatgtagt tcccgaatgg
    28141 gctggagcag gtacagtaaa agtattaata ctagataaaa atagaaaagc agcaacacag
    28201 gaattaataa ataaggttca agaatatata tatccaaatg tggaagaggg tcaaaatcga
    28261 gatggaaaag ctcctatagg tgctattgta actatagcca ctccacaaac attattaatt
    28321 aatgtaaaag ctagttttgt tttttcaaat ggatttagtc aagaaactgt gctaaatatc
    28381 ttaaaaaata aaatagacaa atatctggat aaaatagata ttggaggtac tgtttcttat
    28441 aatgcaattt attcactagc aggttctatg atgcttgcag atgaaggaat acaagatttt
    28501 tctaacttaa caataaatga tggaacaaca aatattattt taaatgacca agttgttgga
    28561 ataggtgaga taatcaatga ggtgatagcg tgataacttc taaaaaaggt agagaaatgc
    28621 ttctaacatt atctcctatc tatgaacaat ctatcataat gcaaagctta tatgaagcta
    28681 taggaagcga atttgataat ctagagttat tggatgaaga aatagaatta caattattcc
    28741 ctcagagcgc aacatgggga cttgaatttt gggaaaatag ggtaggttta tatactaata
    28801 tagatgaaga tatagaagct agaagaagaa aggtcattgc taagcttcaa atgaaatata
    28861 ttgttaaccc tgacagatta gcaactataa taaaaagcta tactggtgca gacgtatata
    28921 taaaagaaaa tatagctcca tacgttttta aagtaactgc taatgtagat gatgttatta
    28981 attatgaaga ttttaaatat ataacaaata aaactaaacc aagtcatctt cattggatgc
    29041 cttcttttgc actcaaattt acagatatag aaaaatttga ggtaaagatg attaatcgaa
    29101 tatttataga ttttagaggg aatataagta atttcttaga tggtatgtgg ttattaaatg
    29161 gtagtaaaaa cttaagtgct tatatacttt ataatgagcc aataagctta aacatgaaaa
    29221 ataagttatt tgtaaaagaa agtgaagtat ttacaaatct taaagtaata attaaaaaga
    29281 atttatatta cctaaatggg atagaaatgc taaatggaaa taaactgctt aatgcagaat
    29341 taagagagga agtgttataa gtggcaaatg cagtaacaac agacattgca agacaaaaaa
    29401 tgtgtaaagc tcgagcgggt gatatagtat tacctactat agttagtatg gtgtttggag
    29461 atggaggagt tgggaatgat ggaactataa tagctccact ttcaagtgat acagcgttaa
    29521 aaaatgaagt gtttaggaaa gatatagaaa attatgtata tccaatccca actacttgca
    29581 gatattcaac tactctatta aaaaatgaag cagaaggaaa aaatataaat gaaataggtt
    29641 taatagattc ggatggtgac ttaatagcaa taaaaacttt tggaaataag tataaagata
    29701 gtgatatgga aatggtattt caaatagatg atgagttcta ggaggtgaat aaatgcctaa
    29761 tgaattaaat tttaataatg aaatagaaca atatttaata actacaccag ctcatgcgaa
    29821 tgagtttaat aatcgacaac aaaaattgtt agacaatgat aaatatttaa ataataaaat
    29881 tgatacaact aaaacagagt taaa
//
LOCUS       DAELJO010000025        29708 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELJO010000025 DAELJO010000000
VERSION     DAELJO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.025"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1029
                     /locus_tag="KPB88_001022"
     CDS             <1..1029
                     /locus_tag="KPB88_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0053320.1"
                     /translation="FREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPFS
                     VKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKYK
                     IAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEIN
                     APVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYKD
                     ILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVALI
                     QTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFMY
                     KLKIYLGG"
     gene            1032..2075
                     /gene="ypdE"
                     /locus_tag="KPB88_001023"
     CDS             1032..2075
                     /gene="ypdE"
                     /locus_tag="KPB88_001023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0053321.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            2090..2395
                     /locus_tag="KPB88_001024"
     CDS             2090..2395
                     /locus_tag="KPB88_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0053322.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2492..2827
                     /locus_tag="KPB88_001025"
     CDS             2492..2827
                     /locus_tag="KPB88_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0053323.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            2859..4181
                     /locus_tag="KPB88_001026"
     CDS             2859..4181
                     /locus_tag="KPB88_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0053324.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            4191..5261
                     /locus_tag="KPB88_001027"
     CDS             4191..5261
                     /locus_tag="KPB88_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0053325.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGKYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            5266..6453
                     /locus_tag="KPB88_001028"
     CDS             5266..6453
                     /locus_tag="KPB88_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0053326.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKDFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(6650..7522)
                     /locus_tag="KPB88_001029"
     CDS             complement(6650..7522)
                     /locus_tag="KPB88_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0053327.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(7916..8107)
                     /locus_tag="KPB88_001030"
     CDS             complement(7916..8107)
                     /locus_tag="KPB88_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053328.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            8327..9373
                     /gene="cobT"
                     /locus_tag="KPB88_001031"
     CDS             8327..9373
                     /gene="cobT"
                     /locus_tag="KPB88_001031"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG0053329.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            9405..9965
                     /gene="cobU"
                     /locus_tag="KPB88_001032"
     CDS             9405..9965
                     /gene="cobU"
                     /locus_tag="KPB88_001032"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG0053330.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            9975..10748
                     /gene="cobS"
                     /locus_tag="KPB88_001033"
     CDS             9975..10748
                     /gene="cobS"
                     /locus_tag="KPB88_001033"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG0053331.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            10768..11382
                     /locus_tag="KPB88_001034"
     CDS             10768..11382
                     /locus_tag="KPB88_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0053332.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      11449..11628
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11757..13256
                     /locus_tag="KPB88_001035"
     CDS             11757..13256
                     /locus_tag="KPB88_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG0053333.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            13295..14671
                     /locus_tag="KPB88_001036"
     CDS             13295..14671
                     /locus_tag="KPB88_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG0053334.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            14672..15643
                     /locus_tag="KPB88_001037"
     CDS             14672..15643
                     /locus_tag="KPB88_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0053335.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTILATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            15675..16745
                     /locus_tag="KPB88_001038"
     CDS             15675..16745
                     /locus_tag="KPB88_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0053336.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            16747..17634
                     /locus_tag="KPB88_001039"
     CDS             16747..17634
                     /locus_tag="KPB88_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0053337.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNHLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            17622..18254
                     /locus_tag="KPB88_001040"
     CDS             17622..18254
                     /locus_tag="KPB88_001040"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG0053338.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            18263..19489
                     /locus_tag="KPB88_001041"
     CDS             18263..19489
                     /locus_tag="KPB88_001041"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG0053339.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNIDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            19486..20094
                     /locus_tag="KPB88_001042"
     CDS             19486..20094
                     /locus_tag="KPB88_001042"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG0053340.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            20084..20659
                     /locus_tag="KPB88_001043"
     CDS             20084..20659
                     /locus_tag="KPB88_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG0053341.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            20652..21404
                     /locus_tag="KPB88_001044"
     CDS             20652..21404
                     /locus_tag="KPB88_001044"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG0053342.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            21388..22509
                     /gene="cbiG"
                     /locus_tag="KPB88_001045"
     CDS             21388..22509
                     /gene="cbiG"
                     /locus_tag="KPB88_001045"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG0053343.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            22506..23231
                     /gene="cobJ"
                     /locus_tag="KPB88_001046"
     CDS             22506..23231
                     /gene="cobJ"
                     /locus_tag="KPB88_001046"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG0053344.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            23228..23980
                     /gene="cobK"
                     /locus_tag="KPB88_001047"
     CDS             23228..23980
                     /gene="cobK"
                     /locus_tag="KPB88_001047"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG0053345.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEINYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            23999..25477
                     /locus_tag="KPB88_001048"
     CDS             23999..25477
                     /locus_tag="KPB88_001048"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG0053346.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIGDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            25507..26412
                     /gene="hemC"
                     /locus_tag="KPB88_001049"
     CDS             25507..26412
                     /gene="hemC"
                     /locus_tag="KPB88_001049"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG0053347.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            26402..27901
                     /gene="cobA"
                     /locus_tag="KPB88_001050"
     CDS             26402..27901
                     /gene="cobA"
                     /locus_tag="KPB88_001050"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG0053348.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASNNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            27918..28883
                     /gene="hemB"
                     /locus_tag="KPB88_001051"
     CDS             27918..28883
                     /gene="hemB"
                     /locus_tag="KPB88_001051"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG0053349.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            29036..29614
                     /locus_tag="KPB88_001052"
     CDS             29036..29614
                     /locus_tag="KPB88_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG0053350.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        11975 a         2985 c         5516 g         9232 t
ORIGIN      
        1 tttagagaga tagataaaaa atataactct aattttttag aagataaaga gttctttaac
       61 ttattttact tacatattgc atgtttaata gaacgtataa agaagaacca caagataata
      121 aatccttttt cagttaaaat aagtcaacag tatccaactg tttttaattt atctattcaa
      181 ttttcaaaaa tcatagaaaa tgagtatcat ataaaaataa gtcaagatga aatagggttt
      241 atagctactc acattgctgt tccttttgaa aagagagaag aagctaattt taataaaaaa
      301 tataaaatag caataatatg ctcttctggt ggaggtagtg catttttgat aaatttaaga
      361 cttaaagaaa tttttccaaa tgctgagata agaaattttt cattactaga agaaaaagct
      421 gtaatagaat ttgcaccaga tttgattttt tcaataacaa atcttttatt tgaaataaat
      481 gctccagtta tacttataaa tgaaatttta gacgaattgg attatttaga tattaaagaa
      541 agtgttagat ttgcagacca tataggaaat atatcaagcc caaaacaata tatattaggt
      601 ctatttgata agaatcactt taggtgtata aaagataaag ctaagtataa ggatatcctt
      661 gatagtatgt caaaatgtat agttgacgag ggagcttgtt ctccaaccta tcccaaagat
      721 gtatgggaaa gagaaagcta tttgagtact atttatacaa atggggtggc tatacctcat
      781 ccaatcgaga tgacaggaaa caaaaatata atatcagtag ctttgataca aactgatatt
      841 atttatgaaa atagggtgcc caaaattatt ttcatgattt cattaataaa gggaaattta
      901 gaacttcata agcagatatc taaatattta agtaagatta tggcaaataa ggatatggta
      961 gacatgctaa ataagtcaca atcttatgaa gagtttatgt ataagttaaa aatctatctg
     1021 ggaggttaga catgaatata gagtttttta agaggttgtc agaagcagat ggtatagcat
     1081 ctaatgaaga agaggttaga catgttttat tggaagaact aaaagcttat agtgataaaa
     1141 ttatctgtga tgggctaggg agtatcatct tttctaaaat aaaagatgag agtgcaccaa
     1201 acgtaatgat ttgtgctcat atggatgaag ttggatttat ggtgagaagt atagacaaac
     1261 ttggtatgat tcacttaata acaataggag gagtaaaacc tcttgcacag tttgtacaaa
     1321 aagttagaat tacaacaaaa gaagggaaga aaatccccgc tgtcataaat gctacatata
     1381 ataatggtaa ggctgaaaat atatatgcag atattggagc atatacagaa gaagatgtat
     1441 ataacctagg gattaatgtt ggggatatgg taacatatac aacaagcttt gaggaattta
     1501 ctttaccaga ccgattagtg ggaaaggctt ttgatgatag gataggatgt tttgtgatgg
     1561 gagaagtttt aaaagaactt aggaaagaaa atctaaattg taatattcac tttgcggcta
     1621 caagtagtga agaggttgga ataaggggag caaagacatc aacacagtta ataaatccag
     1681 atatagtatt tgtaatagat gtagcatgtg ctaaaaatga gtttgtgaga gactatacaa
     1741 atcagaaaca aattgataaa ggtgtaatgc taatgcatag agataggact ctagtcccta
     1801 gtagaaaaat gatagattat gtaatggaaa ttgctgataa aaataatata ccacttcaac
     1861 atgatatgtt tgaatcaggt gggactgatg gaggagaagc acatctcgta aatgaaggga
     1921 aaccttgtgt agtaacttgt gtaccagtta gatatggtca ttgtgcattt tcaatagtaa
     1981 gtaataaaga tttggagaac attataagat tgtatacaca gttaatttta aattttgatg
     2041 aagaacaata taaacatata agaaatttta tctaaaggag aataagaaaa tgtcaacaga
     2101 agtacaaata atggaactta tatcaactgc aggagagagt aaatcgaaag cttttgaggc
     2161 tttaaaaaaa gtgaagtcaa aagattttaa aggagcaaaa ttgttaatag aggaggctcg
     2221 taaaatagat attgaagctc acaggataca aacagaatta ataactcaag atttaaatga
     2281 agatgttgaa agtccagcaa tgagtctttt aatggtacat gctcaagacc actatatgac
     2341 ttctcagcta gcaaaagact tgatagaggc tttgatatat tgtctcgata agtaattgta
     2401 atgtagaaat agattattta tgtaaaaatt cttttaaaag tttagatgct tttaagagat
     2461 aataaaaata caaactttag gaggaattat tatgaaaata ttattatgtt gtgcaggtgg
     2521 attttctact aatatgttaa tgcaaaatat gaaaaaggtg gtaaaagaga gtgctaaact
     2581 caatgaaaaa gattttgagt ttacagctat accagcagat tcaatagaaa gtgaagttga
     2641 tgattgggat atagtactta taggtcctca aatagctcac aaaatagact tcttaaaagg
     2701 aatattagag ccaaaaaata agccttttgc agtaatagat aaagatgtat atggaaaaat
     2761 ggatggagct acagttatga agttggcatt aataacttat aagaaacatc aattagctca
     2821 aggataataa aaataatgag ggaaaaggga gattagatat gttttcaaaa tttgaagcat
     2881 ttatgaatag agtatttatg cctttagctc ataaggttga caatcaacgt caccttggtt
     2941 ctataaaggc tggtatggtt gctatgacac cattcacaat attaggaagt atatttgcaa
     3001 tacttccagc acttccaaat atgcttggag aaaataatcc aataagcaaa ttcatactta
     3061 acaattcagc aatattggat ttaccagtaa gtctttccat aggtttaata ggattatatg
     3121 cttgtatgtg tattgctttt aatctgggga actactataa attatatata ccaggttgtg
     3181 ttgcactatc aactttttcc tttttattcc tagtagcctc atttacacca gatggagctt
     3241 tagaaatagg aaatttagga gctaaagggc tatttacagg tatgatagca gggcttggtt
     3301 ctgtggagtt atataatttt tgtaataaga agaatatcac aataaaaatg ccagaaaatg
     3361 tacctgattt tgtatctagg tcttttgaat taatacctac tactataata gtttgtggaa
     3421 cttttatagt tgctcgtttt atttcattaa aagtatttgg agaattacct ccacagatac
     3481 ttactcaatt tttatctcca ttagtaggaa gtatggataa cccatggtca gttcttgcac
     3541 ttaactttgc tatatgtact atattctttt ttggaataca ttcatcagta tttagtccta
     3601 taactagacc tataatgatt acatttatag ctgaaaacat agcagctatg caggctggag
     3661 aaacaatacc tcatttcttt acagcaggag cttcaagtgc attctttgga tttacaggat
     3721 gtggaattag tataggttgt gtaattgcat gtatgttatc aaaaaatgct tcctataaga
     3781 agattggacg tgtatcatta ttccctgcat tatttgggat aaatgaacca atattatttg
     3841 gagcaccgat aatattaaat ccaataatgt ttataccaca tgtatttggt ggagctataa
     3901 taggtacact accaatgttt tttatgcact ggggatggtt agcaaagcca gtatttgacc
     3961 ctccatatgt aggggtattc ttagaaggat ttttaactaa tggagattgg cattctatat
     4021 tagcaaatgc attgcaatta gtattagcag ttttattata ttggccattc tttaaaataa
     4081 tggaacgtca acaaaataat aaaaaacaaa aagaagaaac taagaatata ttctctaaag
     4141 aagaagaaga cttattggat gatttaggat tggatttcta atattttgtt atgaataata
     4201 caatggagag attagtatcc ttaatctata aaagaggtgt tgacgctctt ttgattaaag
     4261 caaaaaatac taaaagatat ataggagctt tatcaggtag tggtgtatat gtgcttgtta
     4321 ctaaggataa aaattatcaa attctagatg gcagatatat tgatgaagca gataaaaaaa
     4381 cgactggttt cataaaaagg attgtatctc aaggttctta tataccaact ataattgagt
     4441 tattaaaaga attaaatata aaaaagttag caatagaaaa ccaagctatg agtatacaag
     4501 agtatctttc actaaaaaat gaaggttttg aaatcaattt aataacaaat gaattatgga
     4561 agctaagggc tataaaatct aaagaagaaa ttgaacttat taaaaaggct tgtgaaatta
     4621 cagatgaagt atttgatgag gttatttctg aaattaaaaa agatatgact gagctagaag
     4681 tatcagctct aattcagtat catgcattaa aaaaaggtgc aagtggaatg tcttttgaaa
     4741 caatagtggt atcaggagaa agaggtgcaa tgccacatgg aagacctaca aacaaaaaat
     4801 tgaaagtgaa tgaagctata acaatagatt ttggtgttgt ttatcaaggg tatcaatcag
     4861 acatgactcg tacaatatct ataggtaaac ctcctaaaat tattaaagag atatatgatg
     4921 tagtattgga agcacaatta tctgcaatag agtcaataaa ggaaggaact agggcaagtg
     4981 atgttgacaa agtggctaga aaaattatag ataaacatgg atttggcaaa tattttaatc
     5041 atggactggg tcatggtata ggtcttggag atggtgaagt ccccacttta aatccaaata
     5101 gtgaagatat attagtggaa ggaatggtaa tgtcttgtga gccaggtatt tatattccaa
     5161 atgtaggggg agttagaata gaagatgaca ttgttataat agatggaaaa ggtgttccac
     5221 tcaataagac atccaaagag tttataatat taggagagta aaattatgaa atataatttt
     5281 aataaagtat taaatcgtaa agaaggaaat tgtagaaaat ggagcaacca tgtgattaaa
     5341 gaaaaattcg gcttaaatga agatgcaatt cctatggatt tagctgatat cgattttgag
     5401 tgtgctcctg ctataaaaga atctatctta aaaagagcat cagtaggaga ttatagttat
     5461 acttttatag gagatgactt ctatgatgcg attataaatt ggaataaaag aagatttgat
     5521 gtatatatag aaaaggattg gataaaatta acttttggta ctgtttcaac ccttcattat
     5581 atagtccaag catatactaa agaaggagag ggtgtattga taaatacacc tgcttatgac
     5641 ccttttgcag aggctgtagt aaataataat agaaagttgt gttgtagctc attaaaatta
     5701 gataacaata gatattattt agatttagag gatatggaaa atcaaatgaa atacgagaat
     5761 ataagattgc ttatattctg tagtccacaa aatccatcag gtcgtatatg gacaaaagaa
     5821 gaactatatc aagtgtcaga gttatgttta aagtataatg taattttggt aagtgatgaa
     5881 attcatagag atataatatt taaagattat agatttgtat ctttatggaa tgctcatccg
     5941 gaaatttata aaaatagtat aatctgtgta tctcctaata aaggcttcaa cttaggtgga
     6001 ttaaaaacat catatgtatt aataagagat gtaaaaatta gggagacttt attagaaagg
     6061 ctaaaaagta attcaataac ttctccaaat gtatttgcaa taccagcaat cgttgcagcg
     6121 tacaatgaaa gcgaagaatg gctagatgct atgactagtt atgtagagga gaattttgaa
     6181 atagtttatg atttctttga aactaatata ccaaaagcca aagtgatgaa gtcagactct
     6241 tcttttttag cctggataga tgtaagagaa gtgtttagaa atgaagaaga atctaaagac
     6301 ttttttagac atgcaaactt aactatggta gttggaagtt attttgtgaa agatggagat
     6361 ggatttattc gtattaatgt ggggtgtcct agagaaacat taaatgaggc tctaaataga
     6421 atcaaaaaaa cgtatataag tatgtattgt taaatcataa aaaaagtatc tatggtttat
     6481 ataaaaacac attatttagt tatcaaatat tactggaatt ttgtattcca gtaatatttt
     6541 ttgattaagg aatattattg aggaatatta aataatatta tttcataccc ttatattaac
     6601 ttatataaaa atattttatt attttggaaa cgggttagct aacctttatc tagtgtatat
     6661 ttatattgta agaagcagta aaaacttttc ctttactaag tttattgata aacttcttat
     6721 ctttataaat tttgttagaa tttatactat cagcaagacc ataccaaggt tctatacata
     6781 aaaaaggagc agtagaatta gtttcactat aataaggagt ccaaatacca actagaggga
     6841 aatcttccat agatatagtt acatatttac taccatcatt attacatata gatacttcat
     6901 ctatgttggt gtatatcaaa gcatcattct tgaatacttc tggatttaac tgtaagtttt
     6961 ttaattttcc aataggtatt atatcatctg taaaagaacc atttaaattg attttttcta
     7021 catcattttt acttttaaac tcaaggtagt gtttagaaaa atcattttgt tcgtaaaaag
     7081 gaaggttgaa tgcaggatgt ccacctatag aaaagaatat atctttagaa tcagtatttt
     7141 ttacaatcca ttctacctca atactattat catttaatgt atagtttatg attagctcaa
     7201 aagaatatgg atacttcttt aaagtagatt cattatctgt taatttataa gttacaaatg
     7261 tatctccttt atctacaatc tcaaagtcca tatctctagc aaacccatgt tgagtcatgc
     7321 tatataaatt ttcttctatt attgtttcat tatcaaaaag tttacctact atagggaaca
     7381 atataggtga ttgtctgccc caatattttt tattaccatc ccaaagaatg tttttgtcgt
     7441 attttttaga aaatatttta gttagttcag caccaaaact gcttgattcg ataattaaat
     7501 tttcattttg taatatattc atttaaaatc tcctttatta tattttatga gtatattata
     7561 ctataaagta taaagattat taaattttat ttgatattat atttgaaagg tagattttag
     7621 tatattttga aaattttgtt ttgaaaattt gggattattt aagaaatttt gtattataaa
     7681 tagaaggaaa tagataaatt gtatagaata aaatatatta agataagtta ataatgttaa
     7741 atgggaagtg agtttatgga aaaaccaaat acaaaagttg ttaactttca agagtataaa
     7801 gacagaaaaa accaaagaga agtaaataga gacgaacttt taaagcttat aaaaaaaatt
     7861 gtgacttcaa aatagggagt tgttaaactc cctattttaa tgggtttcat aataattaag
     7921 acattctgag tccatctata taatctttga attgtgagaa atctgattca ctacttaatt
     7981 ctatggtttc tgaaccttcc gtataaaaat tcttaagata gatagtataa ttattatcaa
     8041 cacaatcatg ataaatacat ctatatccat cttcatacaa actttttaac ttagaaaaat
     8101 catccacaat atcatctcct taaagttctt ttaaatatat ttttacaaaa agcatctaat
     8161 tgtatgcaaa ttgacaatgt aaaataaagg aaatgaacat aaaatgtaga ataattttta
     8221 tatgtatgta aaaataaaaa gaaaaagttg aaatttttag aaaataagag tattttttat
     8281 ctaaaaagag agtttaaaaa ataaaaaaat gttgggagga taaaaaatga gtttattaga
     8341 aagtatatca aaaaacatat attccctaga taactcttct attgaaaaaa caaagcagag
     8401 attggatagg cttatacatc caactggaag cttaggaaag atagaagata tttgcatgca
     8461 attagcagga atatttggca atgaaaattt tgacacaagt aaaaaggtta taattgcttt
     8521 tgcgggagac catggtgtat atgaagaagg cgtagcacct gaccctcaaa atataacaaa
     8581 attacagttt ccaaattttt cgaaaggtct atgtggagtt ggagtaataa gtaaatttgt
     8641 aggggcagat gttgtagctg tagatgtagg aataaactgt gatgaaaaac tggatggggt
     8701 attagactac aagataagaa aagggacatc aaatatggca aaaggtccag ctatgagtaa
     8761 acaagaagca attagatgtt tagagatagg tattgaaata gcagagcagt gtatagaaaa
     8821 agattataag gttattggta ttggggagat gggtatagca aatactactc caagtacagc
     8881 aataatatct gtaatttcag ggtgtgaccc gttagaagtt actggtatag gtgcaggtct
     8941 taagaaagaa agactaaagc ataaggctga agtaattaga aaggctattg aaattaataa
     9001 tccaaatcca actgatggag tagatatttt atctaaagta ggaggatttg aaataggctc
     9061 aatggcaggt gttatattag gttgtagtgc taataggatt ccagtagtaa ttgatgggtt
     9121 tatttcttat gcagcagcat taattgcata taagataaat ccaaaaacta gagaatatat
     9181 gatagcatct catttatcag cagaatcagg aactaaaaga gcattagaca tattaaaatt
     9241 agaccctcta ttaaatatgg atatgagatt aggtgaggga agtggagctg ctttagcatt
     9301 tgatataata gaagcttcaa actatactta taaaaatatg gcaacttttg atgaaattga
     9361 catgggaaga taaatataaa aatttaatat gtaggagtgt tttgatgagt aaaattattc
     9421 tagtaacagg aggagctagg tcagggaaga gtaactttgc agaaaaacta tgttttgaca
     9481 gaaataataa tacagcttat atagcaacct ctattccatt tgatgatgaa atgaaagata
     9541 gagtaaaaaa acatcaagag aatagaccta gaaactggaa tacttatgaa atatatgaag
     9601 atatatattc cataattaag gagatatata aagaacatga aactgttata ctagattgtg
     9661 taacattatt agtaaataat ctaatgtttg aacacaatct aaatatagaa gaatctagtc
     9721 aagaagaaat aaacaaatta gaacaataca taaaagaaca agttgtcaaa cttattgaag
     9781 aaatagaaaa aacaaatcta tactttgtat ttgtaacgaa tgagttaggt atgggaattg
     9841 ttcctgggaa taaacttagt agaatttatg ctgatattgt aggtcgtata aatcaatata
     9901 tagcttcgaa aagtaatgaa gtttattttg tagttagtgg catagctatg aagataaagg
     9961 agtagtatgc aattatgaaa agatttattt taatactaca gtttttgaca aggataccta
    10021 taaaattaaa tgtaggattt gatgatgaat tttataagtc aatagtatat tttccacttg
    10081 taggttttgt cataggtata ttatcctatt taataggatg gatttctatg cttttatttg
    10141 aaccatttat agcttctata ataataactc tagctggtgt cttaattact ggaggacttc
    10201 atatagatgg attaggagat acatttgatg caatatatag ttatagggat aaagaaaaga
    10261 tgcttgaaat aatgaaagat tcaagattag gtacaaattc acttctagca attatgtttg
    10321 tacttttatt aaaggttgga ttcgtttacg atataataag taataattca ttgtgggtta
    10381 taatttttat gccaatgatt gcaaggttag gagtaatgtt attgacatat aaaactgtga
    10441 caccaagaga aaaaggaatg ggaaatttat ttataggaaa attaactaca agtatgttaa
    10501 taactgctat aatatacaca ttattgatag ttgctcttat tactaaattt atatttttat
    10561 tacctaatat agtgttgatt aaagtattag gttcgattat agtggttttt gtatttataa
    10621 tattattcaa aaaacatatt tataaaaaaa ttgacggagt aacaggagat atattaggct
    10681 gtggaataga actttcagag cttgtatatt taatttacat atatttgtta atttttatgt
    10741 ttttttaaag taaaaagtgg tgataatatg ataagattaa tattaataag acatgcactg
    10801 acaaatgata ataaaaaagg aagattatca ggccatataa atagttgtat tagtgaagag
    10861 ggcaaactac aaataaataa aattaccaga tatctgagta atgaaaacat agataaggtt
    10921 tatacaacac catctacacg tactaaagat acagtagaaa aaatatcaaa attgaaatta
    10981 ttagaaatag aagaaaaaga agctttaaga gaaattagtt ttggagactt tgaaggaaga
    11041 acttttgaag aaattaaaat caaatatcca aatgagtttg aaaaaatgat aaaagaaggt
    11101 aacaactata gatatccaaa tggagaaagt ttgatagact cttataaaag agtggcgaaa
    11161 gaaattgata atataatttt agaaaatgat agtaatttag atacaaagac aatacttata
    11221 tgttctcatg caggaacaat aagaaatata ataacacatt tgatttctgg tagttataag
    11281 tatcactgga actttaaaat tgataatgca tctattacag ttttagaaat tgatggtgga
    11341 tttgctgtaa tagataaaat gaattttaca gacttcatat aatttaatca aaaccaaaca
    11401 ttacaatctg tgtttgtaat atatctgtaa aattagcaat gaaaatagaa tattagatta
    11461 aaattaggtt cttaattaga tgttaaaagg gaaaaaggtt agatgccttt gcagcccccg
    11521 ctactgtgaa accaacgaaa ctataaatac cactgtcagt ttgatgggaa ggttatagaa
    11581 gtaggatgaa gttaagccag gatacctgcc taatttaatt taaaacaaga ggtcttcggg
    11641 gtgaaagatt tttaattaat atatataaaa gtcctatctt tagaagtatt aggacttttt
    11701 ttatttgttt tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc
    11761 tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat
    11821 ttaagcaaga tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt
    11881 ttataactaa agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga
    11941 aaatagagcc aatagctgat atgaatccag ttttattaaa accttctgga aataataaaa
    12001 gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt
    12061 ataaattgga gttagtagat gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg
    12121 atattgtagt tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata
    12181 tctctaatat gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg
    12241 atagaggagg cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga
    12301 aaagaagagt aaaaggtgta ataatcaata aattcagagg aaaaaaagag cttttacaag
    12361 atggtgtaaa gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata
    12421 cagatataaa aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata
    12481 aaagtgatat acacatagaa ataattagaa ctccacatat gtcaaatttt acagatttta
    12541 atatatttga gacacaagaa gatgtaagta ttagatatgt agactatgga gaatctttag
    12601 gaaatccaga tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata
    12661 taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat
    12721 ttgggatatg tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg
    12781 aaagtgaaat tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga
    12841 aagaaaagac tacaacacaa gtagaagcta ctattgttga atgtatcagt gaatacatga
    12901 ataatcttaa gggaaaaaaa gtaaaaggtt atgaaattca tatgggaatt acaaagcgag
    12961 gtaatgatgt aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata
    13021 ctgagggaag tgtaaataaa gaggggaatg taattgggac ttatcttcat ggaatatttg
    13081 atgaaataag ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag
    13141 aattagaaag caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg
    13201 ctgatttttt aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat
    13261 gacctttatt tataatttaa gtaggaggtg tattatgaaa aaaattttaa ttgcgggtac
    13321 taatagtgga gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag
    13381 aaatatgaaa gtacagccat ataaagtagg gccagactat atagaccctt catatcacac
    13441 ttttataact ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat
    13501 aaagtatata gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat
    13561 gggcttatat gatggctttg gaatagattt agataattgt acaagttcac atacttcaaa
    13621 ggtattaaag tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc
    13681 tgctatggta cttggatata aggaactaga taaagatgta aatataaagg gtgttatagt
    13741 aaataatgta aagactaata gccattatca aataattaag agttctatag aaaaatattg
    13801 taatgttgaa gtattggggt attttcctcc aaataacaaa ttctctttgg aatctcgaca
    13861 tctaggatta gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc
    13921 agatgaaatt gaacactata tagatattga cagaataatt gaaatatcag aaagcgatga
    13981 atttgagagt gattttgatt ttcaaacttt aatagaaaat aataaaataa aagaacatgt
    14041 taataataaa tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa
    14101 tatagagctt ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgt
    14161 atcagtgcca aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa
    14221 agaattagaa gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat
    14281 acctatttat gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga
    14341 agaacaagaa tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt
    14401 aaagagattt gggtactgta ttggtgtagc caaggaaaat actcttttat ctaaaaaagg
    14461 ggaatctatt aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt
    14521 atattcaatg agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag
    14581 taaaggaaac actttggcta catatcttca tactcatttt tataataact taaattgtat
    14641 agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt ttatctattt atataggata
    14701 tgtgacagac ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg
    14761 taaacttata aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt
    14821 aaaatttggg ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta
    14881 tgttatagtg aagttattta gttttaatat attattgagt attgtagtga atagttttat
    14941 aatttataca atattagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt
    15001 attaaaaact gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga
    15061 tactactaat ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa
    15121 tactgtagat ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc
    15181 tatggcctat aaagctatca atacattgga ctcaacagtt ggatataaaa ataaaaagta
    15241 taaagatatt ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag
    15301 aatatctgtc attcttatga caattggaag tttcttttta aattataact ataggaactg
    15361 ttttaaaatc tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga
    15421 aggtgcagta tctggtgctc taggaataca actaggaggt accaacgttt attttgggga
    15481 aaaagtatat aagccaacaa taggggataa gcttagggag atagataaag aagatataat
    15541 taaaactaat aaaattatgt atgcatcttc atttgtatct atgttggtat ttacactaat
    15601 atatgtgtta tgtaggatga ttttaattaa cttaattata taaattattg tttgttaact
    15661 taagggggac atagatgaag gatttagggc atggtgccaa tgtagatgaa atggcaagat
    15721 tatatgggaa aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat
    15781 taccaaattt agagagatac attttaaaag gattagaaga gtgtaggaat tatccagata
    15841 taaattatac aaacttgaga gaaaatatat caaaatatat agatataaat ccagacttta
    15901 taatacctgg caatggtgca acagaagtga tttatttgct tatgaagagt attaaaaaaa
    15961 aattggctat aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt
    16021 tagatattat agatttagag ttagatttag aaaataactt taaacttaat atagatataa
    16081 taaaagaaaa tataaaaaga tttgatagtt tatttatatg taatcctaat aatccgagtg
    16141 gtaatgtcca agatttaaag gaacttgttc atttactaga taaacataat aaggtactaa
    16201 taattgatga aactttcatg gaatttgttg aagatgaaag taaatatagt ttggttaaat
    16261 atatagaatc aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc
    16321 ctggtttaag gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg
    16381 aacacaaaga gccatggact atcaattctt ttgcagatat actatccaat tttatttttg
    16441 aagataaaga gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatatgt
    16501 tacaagagtt gagaaacata agaaatataa aggtctatga tacagatgct aattttatct
    16561 tgataagaat atataaaaaa acaactaaag aattgaaaaa agatttgttt aagcaaggaa
    16621 acatattagt tagagatgca tctaatttta taggattaga tgatagtttt ataagagtgg
    16681 ctataaaatc tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg
    16741 attaaaatga aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata
    16801 attgatgatg aagaatattt atgttcatat gcaatagata tgtattctaa agtgtatatt
    16861 gaagaaaaat tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag
    16921 gtatttgaaa aatttaattt acctaaaaaa tatactaaaa atatttcttt aaatataaat
    16981 agtaaaatac ctgttggaaa aggaatggca agttcaactg ctgatatagg agctactata
    17041 aaggcaacat tatcattgat agataaagat ttaagtagcg aggagatttc taaattagca
    17101 gcagaaatag aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta
    17161 aatggaactg ttataaaata cctaggaaat ctgaccaatg cgaaagttgt aatacttgaa
    17221 ccaaacaaag tactagatac tatgaaaatt agattgaggc aagattataa taaattaaaa
    17281 gtagagaata aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa
    17341 aatcatttat ctctagttgg agaggcttgt actttgagta gcttagcaaa tgagaatata
    17401 gaaaaaaaag agtatttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga
    17461 gtaaatattg ctcacagtgg aactgtagta ggtattttaa ttgataagtc tatgaatgat
    17521 aaaaagatga tagatgcctt atgtgaatct aacataaatt ctgtctataa taaaatttat
    17581 acacaaaaca taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc
    17641 ctatgaaaat agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac
    17701 cagattataa atttaagaat aatatcgaag aaaaaataat aaaaagagct atacatacta
    17761 ctgctgattt tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa
    17821 tagatgcatt aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta
    17881 taaataaaag aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg
    17941 aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg
    18001 cagctaaaga agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata
    18061 aagttatgga gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg
    18121 taggttttgt aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata
    18181 taatttctaa aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat
    18241 tatatactat gtaggtatct atatggaaga atatgtatat atagatggaa aaaaatatag
    18301 aagaggatat actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt
    18361 gattacaaaa aatagaatca atactataaa tatagatact cctaagggga taccattact
    18421 actaaaagtt gataatataa atatttctga tacatttgtg gaatgttcca taaaaaaaga
    18481 tggtggagat gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt
    18541 agctaaaaat gataagaaca aagggtatct tactctaaag gatatagata gtttaagcac
    18601 caatagtgag tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat
    18661 tggtgtggtt acgaaaaaag gtcttagcgt ggatgttggt aaacctgcta taaatccgac
    18721 tccacttaaa atgataaatc atgaaataag aaagcttata ggagataatt ttgaatctat
    18781 acttggaaat gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc
    18841 aaaaaaaaca tttaatccaa gattaggtat agttggagga atatctataa taggtacaac
    18901 tgggattgtt gaacctatga gtgatgatgg ttggaagaag tctctatcta tagaacttca
    18961 gatgaaaaaa gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca
    19021 gtttataaga gaaaaattaa acatagatat taagtatgta gttagggtaa gtaattttat
    19081 aggctatatg attaaagaag ctcaaagaat aggatataaa aaaatcttaa tggcaggtca
    19141 tataggtaaa tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga
    19201 tgctagaagt gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt
    19261 aaataaaata aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga
    19321 atatagagaa gtatataata tattaagtaa taaatgtagg gagagagtaa agcaatactt
    19381 gaatgaagat tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt
    19441 attaggtaaa tcggataata cagatgattt agtggaggtc tttatatgat aaacataata
    19501 ggtctaggac ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact
    19561 tctgatgtat taattggtgg taaaagaaat ttagagtcta taaaaaattt tgaaggagaa
    19621 aaaatagttc tggattctaa tttaagagaa attatagagt acataaataa caataaagaa
    19681 aagcaaatat caataatagc atctggggac ccattaatat atgggatagg tagatatcta
    19741 tctaaaaata ttgataataa gatgttaaac atggtttctg ggattagttc attacagtac
    19801 atattttcaa aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggcaaa
    19861 gtaccagatt ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag
    19921 ataggaccaa aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt
    19981 gtaggagaaa atctgtcata tgacaatgaa aaaataacaa tagcaaaacc agaagagata
    20041 ataagaatgg acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgagtt
    20101 tataacaggt aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt
    20161 aaatttagta aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt
    20221 tgaagctgca tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgatgc
    20281 tatagagctt atagagaaaa ataaaaaaaa attcaactta aaaaatatag aagtgataaa
    20341 aggatatgct ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg
    20401 aacaggaaat aatctggaag aaataataga atggtctaaa aaaaatctcg ttacaggagg
    20461 aagacttgta gctaatttta taataataga tacatttaat caaactttaa aactgcttaa
    20521 aaagcatgga tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tggagaaact
    20581 aggaaaaggt gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg
    20641 ggaaaatgat gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga
    20701 taacattaaa aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac
    20761 ttgttaatcc tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac
    20821 atatggattt acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg
    20881 ttgtaagact gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag
    20941 atttaaataa attgaatata gattatgact gtactcctgg agtaagttct tttttagggg
    21001 cagcatcatc tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa
    21061 caagaatgga aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc
    21121 atcagacatc tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat
    21181 tactggaagg tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg
    21241 cagatgaaaa aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca
    21301 acataaataa gactgcttta ataatggttg ggagattttt aggagaagag tataataatt
    21361 caaagttata tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt
    21421 attacagaaa atggaaaaaa attagctcta aagataaatt ctctagtaaa taactgtgtt
    21481 atatatcaag taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtacataca
    21541 gttcaaaaga agttaagtga ttttgtagga gaaatattta gcaaatttga ttacatagta
    21601 tttataatgg ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt
    21661 agtgaccctg caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt
    21721 ggtcatattg gaggggcaaa cagcttgaca cagtacataa gtagtcttat tgatgctaat
    21781 ccagtaataa caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa
    21841 aaactaaatg cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta
    21901 gtgaataata aatgtgttgg attatatatt gatgaaggat atcaagttga tactagggga
    21961 tttaaactta tagaaagtaa ggatgagtta agagatagtt tttcaaacac atatatagaa
    22021 aaattagttg tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaaccta
    22081 gataataaat ttgaacattg ctatagtacc aaaaaaataa taaaggtaat tccaagagat
    22141 atagtgatag gaattggttg tagaagaaac acagatagtg aactgatgta taattcactt
    22201 gaagagtttc ttaataaaca aaatatagat attagagcaa taaacacagt tggaagtata
    22261 gaactgaaaa aagacgaaaa agcaataatt gaattatcaa aaatattgaa ggtgccattt
    22321 aaaacatttt cagtagatga aatatctaag gttgataatc tttttgataa atctgatttt
    22381 gttaaaaaaa atgtgggcgt gtattcagta gcagaacctg tagcatatct tttgagcaaa
    22441 ggtaatttgt tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata
    22501 aaaatatgat atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag
    22561 caataaaagc aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa
    22621 tagaagaatt tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa gaagttgata
    22681 gatgtaaaca agcagtagag gaagcaaaaa aaggaaaaaa agtagcagtt gtaagcagtg
    22741 gtgatgctgg aatatatggg atggcaggtc ttattttaga attaatatct aaagaagatg
    22801 aagatataaa agtaaaggta attccaggtg ttactgcaag tattggtgca gcagctatac
    22861 ttggagctcc tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt
    22921 gggaagtaat tgaaaaaaga ttgagattgg ctgctgaagc cgattttgta atttgtttgt
    22981 ataatccaag aagtaaggga agaagtgaac atctaagcaa agcatttaaa ataatgggtg
    23041 agtttaaagg tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag
    23101 aaaagtttgt atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg
    23161 ttataatagg aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt
    23221 atacagtatg attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat
    23281 aaatgaactg aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact
    23341 tgcacaacaa catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa
    23401 atttataaaa gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga
    23461 agtatccaaa actgctatta cttgtgcaaa actattgaat atagattata taaggtttga
    23521 gagaaaatct ttaattgaag aaataaatta tgataataaa tttatagtag atacaattga
    23581 agaagcctgt gaaatagcaa ataaaatagg gacaaatata ttcataggaa caggaagtaa
    23641 aaatttgaac agatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc
    23701 aacgagtgaa gtcatattaa gttgtgaaaa attaggttta agtgcagata atatagtagc
    23761 tatgaaaggt ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga
    23821 tttagtcata acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg
    23881 taaaactttc aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt
    23941 atttaatgac ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat
    24001 gaagaaagcc ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac
    24061 aatagatata tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc
    24121 ttatacatca aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa
    24181 tccaatacag gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac
    24241 acttcatatt atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc
    24301 ttctaaattc aaaaagattg tattaggaag acctttgctt acacatattg gagattatca
    24361 ggaagctgta gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt
    24421 atttatggga catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat
    24481 gcttagggat aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga
    24541 acatgtaata agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat
    24601 gttagttgca ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa
    24661 aacaatatta gaagaaaatg gatttaacgt aagagttcat ttaaaaggac taggagaaaa
    24721 ttcttatata caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgcgaagtt
    24781 ctatggtata ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac
    24841 tttaaagaaa ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc
    24901 tttatcaata gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc
    24961 aatgagtttt gatgggaatg aaaaaagttt agcttgggac aatgtggcac aagaaattat
    25021 atcagatgtt aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta
    25081 tagtacatat gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc
    25141 aggaatttca tcattttcca atatagcatc taatcagaat tttccattag tcatggataa
    25201 agaagcttta gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca
    25261 aaattataat tcaatagtat tgatgaaagt atataaaaat tttaaagaaa taataaaaaa
    25321 attagaaaag tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag
    25381 cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc
    25441 tactatacta gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga
    25501 cttaatatga atatagtggt tggaactagg ggtagtaatc tagcgcttat acaaacagaa
    25561 tgggtaataa atgaattaaa aaagaagtat ccagaaatta gttttgagat taaaatcata
    25621 aagacaaaag gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg
    25681 tttgtcaaag aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt
    25741 atgaaagata tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga
    25801 gaagacccaa gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct
    25861 catggagcag taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga
    25921 cctgatttaa atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa
    25981 gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttacaa
    26041 gataaaataa gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct
    26101 ttagctatag aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag
    26161 gatgagaata cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga
    26221 agttgtcata ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact
    26281 ggcttatatg gagattcaga aggaaaaaaa gtagtaatca agtctataga tggagatatt
    26341 tcttctccta gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat
    26401 tatgaagggt aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt
    26461 aaaaggttta gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag
    26521 tagttattta agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaaa
    26581 taatcatatt ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg
    26641 aaaaattgta actcgtctta aaggaggaga cccttatgta tttggtagag ggggagaaga
    26701 agcagagact ttagtagatg aaggtataga atttgaagta gttccaggta taacttctgc
    26761 tataggaggt ttatgttatg cgggtattcc tataacacat agagaccatg cttcatcatt
    26821 tcatgtaata acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct
    26881 tgcaaataac aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag
    26941 tgaaaactta atgaaagaag gaaaagataa ggatacacct gttgctctta taagttgggc
    27001 aactagatat aatcaaagag ttgttacatc tacacttgaa aatgtttatg aaactgctat
    27061 taaagaagaa gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga
    27121 aaagttgaac ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc
    27181 tagaaatcaa aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga
    27241 aatacctact attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa
    27301 aaatataaat aagtataatt atctaatact aactagtaaa aatgccgtag aaatcttctt
    27361 tgaaaagata tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc
    27421 tataggttct gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc
    27481 aaaaaaattt gtagcagagt cactgtatga agagttagct cctattttaa atgatgacga
    27541 taatatactt atacctagag cagaaaatgc tagagattat ctggttgaca aacttaaggc
    27601 tatttgtgaa gtaacagaag tacatatata tagaacagta atagatgagg agaaaaaagg
    27661 tgagatatta gatattttaa attctaatga catagattat ataacattta ctagttcctc
    27721 aacagtaaat aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat
    27781 aaaaacacta tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt
    27841 gtacaaagaa tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta
    27901 attttggagg cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca
    27961 gaaatttggt aagagaaact aaggtgaatg tagaagattt aatatatcca ctgtttgtag
    28021 tagaggggga aaatataaaa gaagaaatta aatctttacc agatgtctat catttatcaa
    28081 tagatatgct agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt
    28141 tatttggtgt tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa
    28201 tagttcaaaa ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag
    28261 atgtctgtat gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt
    28321 atgttgataa tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag
    28381 caggagctga tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag
    28441 aagctttaga tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg
    28501 cttcaaatta ttatggacct tttagagagg cagctcattc agcaccatct tttggagata
    28561 gaaaaacata tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg
    28621 atatattgga aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta
    28681 taagaagagt taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat
    28741 actctatgct taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat
    28801 caattacatc tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca
    28861 ttgctaaact gttgaaaaaa taaattctag tagtataaag aatatcaaaa agtataggaa
    28921 ttgtaattaa ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact
    28981 aatttgcaat ttctatactt tatttatgta actaaataaa atttggaggt agaagatgct
    29041 atatcctatt aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt
    29101 tgcatatagg aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa
    29161 acaaatttta aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta
    29221 taaagaaata tatataaaag atgcttctgt aataattgct gccacaaata atagagagtt
    29281 aaatatggaa ataggattat attgtagagc aaaaaataag ctagtgaatg tagttgataa
    29341 tatagaaata tcgaacttta ctgttccatc atatataaaa agaggagatt tacttataag
    29401 tgtgtctact gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga
    29461 gagatatact gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt
    29521 tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg cttaatatgt tggtaacact
    29581 tgacctagaa gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag
    29641 gtgttgacgg attattttta agatgatata gtattacttg tccttaagaa aagggcaaac
    29701 ataaaaaa
//
LOCUS       DAELJO010000026        29069 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELJO010000026 DAELJO010000000
VERSION     DAELJO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.026"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            36..425
                     /locus_tag="KPB88_001053"
     CDS             36..425
                     /locus_tag="KPB88_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0053351.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            418..1509
                     /locus_tag="KPB88_001054"
     CDS             418..1509
                     /locus_tag="KPB88_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG0053352.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            1629..2105
                     /locus_tag="KPB88_001055"
     CDS             1629..2105
                     /locus_tag="KPB88_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0053353.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            2491..2958
                     /locus_tag="KPB88_001056"
     CDS             2491..2958
                     /locus_tag="KPB88_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0053354.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(3018..3590)
                     /locus_tag="KPB88_001057"
     CDS             complement(3018..3590)
                     /locus_tag="KPB88_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0053355.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(3653..4750)
                     /locus_tag="KPB88_001058"
     CDS             complement(3653..4750)
                     /locus_tag="KPB88_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0053356.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(4764..5453)
                     /locus_tag="KPB88_001059"
     CDS             complement(4764..5453)
                     /locus_tag="KPB88_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0053357.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(5618..6784)
                     /gene="rpoD"
                     /locus_tag="KPB88_001060"
     CDS             complement(5618..6784)
                     /gene="rpoD"
                     /locus_tag="KPB88_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG0053358.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(6802..8592)
                     /locus_tag="KPB88_001061"
     CDS             complement(6802..8592)
                     /locus_tag="KPB88_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG0053359.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(8847..9632)
                     /gene="aroF"
                     /locus_tag="KPB88_001062"
     CDS             complement(8847..9632)
                     /gene="aroF"
                     /locus_tag="KPB88_001062"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0053360.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(9613..10119)
                     /gene="folK"
                     /locus_tag="KPB88_001063"
     CDS             complement(9613..10119)
                     /gene="folK"
                     /locus_tag="KPB88_001063"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG0053361.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(10112..10477)
                     /gene="folB"
                     /locus_tag="KPB88_001064"
     CDS             complement(10112..10477)
                     /gene="folB"
                     /locus_tag="KPB88_001064"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG0053362.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            complement(10488..11279)
                     /gene="folP"
                     /locus_tag="KPB88_001065"
     CDS             complement(10488..11279)
                     /gene="folP"
                     /locus_tag="KPB88_001065"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0053363.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(11343..11915)
                     /gene="folE"
                     /locus_tag="KPB88_001066"
     CDS             complement(11343..11915)
                     /gene="folE"
                     /locus_tag="KPB88_001066"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG0053364.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(11941..12642)
                     /locus_tag="KPB88_001067"
     CDS             complement(11941..12642)
                     /locus_tag="KPB88_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053365.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRKNISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(12676..13419)
                     /locus_tag="KPB88_001068"
     CDS             complement(12676..13419)
                     /locus_tag="KPB88_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG0053366.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(13409..14758)
                     /gene="pabB"
                     /locus_tag="KPB88_001069"
     CDS             complement(13409..14758)
                     /gene="pabB"
                     /locus_tag="KPB88_001069"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG0053367.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(14760..15344)
                     /locus_tag="KPB88_001070"
     CDS             complement(14760..15344)
                     /locus_tag="KPB88_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG0053368.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(15368..16087)
                     /locus_tag="KPB88_001071"
     CDS             complement(15368..16087)
                     /locus_tag="KPB88_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0053369.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(16115..17179)
                     /locus_tag="KPB88_001072"
     CDS             complement(16115..17179)
                     /locus_tag="KPB88_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0053370.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(17323..18132)
                     /locus_tag="KPB88_001073"
     CDS             complement(17323..18132)
                     /locus_tag="KPB88_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053371.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEVRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(18374..19240)
                     /locus_tag="KPB88_001074"
     CDS             complement(18374..19240)
                     /locus_tag="KPB88_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0053372.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(19760..20449)
                     /locus_tag="KPB88_001075"
     CDS             complement(19760..20449)
                     /locus_tag="KPB88_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG0053373.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGIVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(20442..20813)
                     /locus_tag="KPB88_001076"
     CDS             complement(20442..20813)
                     /locus_tag="KPB88_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG0053374.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(20995..22341)
                     /locus_tag="KPB88_001077"
     CDS             complement(20995..22341)
                     /locus_tag="KPB88_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0053375.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(22523..23116)
                     /locus_tag="KPB88_001078"
     CDS             complement(22523..23116)
                     /locus_tag="KPB88_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG0053376.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(23181..24650)
                     /locus_tag="KPB88_001079"
     CDS             complement(23181..24650)
                     /locus_tag="KPB88_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0053377.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(24761..26152)
                     /locus_tag="KPB88_001080"
     CDS             complement(24761..26152)
                     /locus_tag="KPB88_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG0053378.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(26318..26566)
                     /locus_tag="KPB88_001081"
     CDS             complement(26318..26566)
                     /locus_tag="KPB88_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053379.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            26832..28961
                     /locus_tag="KPB88_001082"
     CDS             26832..28961
                     /locus_tag="KPB88_001082"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG0053380.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNYEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         9201 a         5046 c         3070 g        11752 t
ORIGIN      
        1 tcgttttcat ttataaagca taatggaggt tttttatggt tttaaattta gattttaata
       61 gtgataaatc tatttatgta caaattaaag aagaaataac taaggctata gcttcaggag
      121 aattgaaaat aaatgaatca cttccatctg taagaagtat ggctgaaaat attggtgtaa
      181 atttacatac agtgaacaaa tcctacaatg aattaaaaga agagggattc ttaaatattg
      241 atagaagaaa aggtgctatt gtagctcaac taccaatgaa aatagataat acaactcgtc
      301 aaaaaaataa acttgacttg gaattattgg tggcaaaatc ttatttatca ggtattagta
      361 aagaagaatt tttgtcacta agttcaagtt tatttgacaa gtatgaggtg aaatattatg
      421 agtaagattg aatgtctatt tacaatattt ccagttctaa ttttgatgta tgctacattt
      481 tattttatgc cctattttgt aggtaaaaaa cacatctatg gagtttctat agaccaagag
      541 cataaaaatt ataattactt tataaagtta gataaagaat ttaaaaaact attatcttta
      601 ggttttatta tagactttat attagttttt attctggtct ttacatttaa taaattggaa
      661 ctttcctatt ttattggtat tataggtttt ttattatatg aaagtatact atatattcat
      721 acacataaaa aagctaagaa gcttaaatca gaaatttatg ctaaattagg tcatatagat
      781 atagattcaa aattaatagt agatatggat tttataaata agaaaaataa aataataaaa
      841 aaatttaaaa taatctattt aataccgata ctatttactt tgggaatgag tatttttata
      901 gcatttaatt ataatcaatt gccagactca attgccactc attggaacat aaatgggtct
      961 cctgatgttt ttatagataa atcatttctt aatgtattta aattaatcgg tactgacttt
     1021 tgtttaatgg ttttattata tataacttcc attggatcca taaagtcaag gattaagata
     1081 gatacaaata gaattgaaga aagtagagtt gaaaacatta aatatttaaa taaaattggt
     1141 tatttgtttt taatattgat gattataatg acaacacagt tttttactac tcttttatca
     1201 ataaaaacaa aactatcaac tgctatgaat attacaacgc ttttagttat aatttattta
     1261 atagcaacat atattaattc cccaaattta aaattcaact catcttattc tccagaagaa
     1321 gatgagaagt attggatagc aggtatcatg tataataatc caaatgaccc atcattaatg
     1381 gttaataaaa ggtttggtat tggatggact ataaattttg gcaatcctct tggaaaaata
     1441 ctatatattg ctatagcttt acttttaatt ttttcattgt ttagcctaat aaaatctctc
     1501 ttactttaaa ataccataaa aggtatctta cttgtattat tgggtattat attttgtagg
     1561 ataagaaata tttcatatta tcgttgaagt atagtattta tatattacta agaatggaga
     1621 ttttcataat gttgtcaaaa gctgaaatca aatattttaa tgaatgtgct tctgagatac
     1681 tttcttctga aaaagttcaa cttatgagaa catttcctca tcatggcaat gtttcatgtc
     1741 ttgaacatag tttgtcagta gcatattata gttatctatt atgtaaaaaa ttgcacttaa
     1801 gtgttgatat tcaaagtgtt attagaggag ctttactaca cgactttttt ctttatgatt
     1861 ggcattacaa aggtgataga aaaggattac atggatttac tcaccctaga gaagcattaa
     1921 aaaatgcaac tttatttttt caaataaatg aaaaagaaac ggacataatt ttaaaacaca
     1981 tgtggccact tactgttaag ccacctagat acaaagaagc atttattgta tgtttgttgg
     2041 ataaattttg ttgtttagtt gagactttaa agatacattc tttattatct ccttaccatg
     2101 tataggacaa ttcccacaac acttttataa cttaattttg aagaggtatt aagtattata
     2161 ttcaacttga tacctcttta ctatttatta ttttatataa taatattttc tttataatat
     2221 cgttataaat aacaacttct accctcgata acttcccctt cttatagaca tgcttactac
     2281 cttaaataac aagaaaccag ctattaaatt tattagtatt gtttgtacta ctctaacaat
     2341 aatatcataa ataaacctcc aaatagcttc tctttcacta ccttttatct taaactatat
     2401 ttttttctta aaatttgtaa aaaacatagc ataatcttta taaatgttct aaaatttaat
     2461 ttaagcatca aaattagaaa ggatgatatt atgtttaaag aaatgagatt gaaaaaaaga
     2521 gaaatgacaa aagaagatac tgttgaagta ttaaaaaatg gtgaatttgg tactttttcc
     2581 actatatctg aaaatggtta tccttatgga gttgctgtaa attatgtata ctttaatgat
     2641 tctatttact ttcattgtgc aagaaatggg cataagttag ataatatatc gaaaaacaac
     2701 aaagtttctt ttttagtagt tgctaatgaa agtgttattc cagataaatt tagtactact
     2761 tattctagtg ctatagtttt tggaaaagct tgcactgtag aaaatgaaga aaagaaaaat
     2821 gctcttgtag aaataataaa aaaatattct aaaggattct ttgaagaagg catgaagtac
     2881 atagagaagg atatgaactt aacaactgtt gttaaaatag aaattgacca tatttctggt
     2941 aaagcttcac gtttataatt aagtaaaaat acctaaaagt atatacagtc gcttttaggt
     3001 atttttatat gatattctta tatgttaagt ctttttaggt aatctcttat acctatcttg
     3061 gcatcatcat ttggtcttat atcagaatta tctcctgcta tatgaagtat tccatacgct
     3121 ggataacctc ctcttactga acatggaact gacaaaagag tcattcccat atgttgggca
     3181 aatatattca tagcttgcaa agctaattct cctcctccat gttgtgaacc tgctgtagca
     3241 aaacaaccaa atattttacc actcaaagga gcacctaccc aaatatcaga aaaactatcc
     3301 ataaacatct tcattggtcc agatacattt ccataatatg ttggtgaacc cataaatata
     3361 gcatcataat ctaatatttc ttttccagat ttaattacat ttatactttc aaattcgtct
     3421 ttgtattctt tggaatttat caaataatat tgaggaagtg tttttgcaac ttcgtcactt
     3481 actctaaata tatctacctc gatattcatt tcttcaagcg cttctttata ttctctagct
     3541 aataaatatg tactcccaca aacactatga aaaattactg ctactttcat cattatcccc
     3601 ctaattaaac tcaaatttat tttttatata gtatttttat tatcaaacac tgctaaatag
     3661 tactaaatgg attcaaatat acattactct ttattacttc aatttcacta aattcatcta
     3721 aaaatctctt cataacattt ttgaatatat cttctgtatc aaaatgacca caatccacta
     3781 tacacattcc aatatcaagt gcatcttgtg cctcatgata ctttacatca cccgtaatta
     3841 gtacttctgc accttgtctc ttagcttttt ttacaaattc tgaaccagct cctgttacaa
     3901 cggctacttt cgttatgtct gtactaaggt ttccaactac tcttatgtgt ttcatattta
     3961 atttctcttt tattttcctt gaaagacttt ctaatgtcat acttttatca agtttactta
     4021 ttctacctag tccaaatatc tcacctttat tttctaactt gtacaaatca tatgcaactt
     4081 cttcataagg atgtgcatct aacatagaac ttattacccc acctaaaagc ttctgtggta
     4141 ctacagtttc aattttaact tcatttacag tttctatatc atttacactt ccaagaaacg
     4201 gattagagcc ttctaatggc ttaaattgtc cttcaccttc tattgaaaac gtacaatcac
     4261 tataattccc tatatgacct gctcctgaat tacttaaaac ctttctaagt tcatctgaat
     4321 agttatgtgg aacgtatact gctaatttat acaaattttc gcttttagtt atatctaaaa
     4381 cctttgaatt accaaattcc ataatttcca taaaatagtc atttagacca tcaaatgcta
     4441 tatcaaaatt cgtatgcata gaataaagtg atatatcatt tttaataagt ttttggataa
     4501 gtctgccttt taaatctcca ctattaattt tattaatttt cccaaatata aaaggatgat
     4561 gtgtcacaat taaatctata tttttggata tagcttcatc aataacatct tcattagctt
     4621 ctaaagatac caataccttt tttacatcca tatcaaaatc tccaactata agacctacat
     4681 tatcccaatc ctctgctaag tttagaggat actttttttc aatttttcta gttagactct
     4741 ttaatagcat atgtacctcc tatttatttt attaaatttt caagtttttt tattgcaact
     4801 tcgctttcct ttctcttctt atctatagct tccccatttt tcccttctaa tttattaact
     4861 atagaactat atgtctttat ctttttttct ataaaatcat taaaaagaga atctttgttt
     4921 tctaaaagct ttattccaac ttcataatat atctcatctt ctattatagt atttttacct
     4981 gtatatttgg caacaattat ctcatatatt ctaaaatctt ctctcactaa aacttcttct
     5041 aaaatttcat atccgttgtt taaaaggtag tatctaagtt cttcttgtgc ttgcattggc
     5101 tgtaatatta atttttctac attatgtgct acttcttttt tagcctcgag aagctcactt
     5161 atcaagattc cacccatacc agcaattatc acttcttcta cttcccctat ttcaagtatc
     5221 tctataccag aacccaatcg taaatcaact ttatctaaaa gattgttctg tatgacttct
     5281 ttatgtgcat tgtcaagagg tcctttatta acatccgcca gcactgcaaa tggtactttt
     5341 ccctctttta acaaatatac tggtatgtat ccgtggtccg tccctatatc agctattttt
     5401 ttaccatctg acactaatga tgctattttc aataatctat ctgttaattt caaatataat
     5461 tctcctttca attcccttta tttataggat ttaatccaag ttatagatat tagactgaaa
     5521 taactacaaa aataaaatta aatctttact acaaaatagc catgagatat cacttatctc
     5581 atggcattta tcacaaaaac atcaatgatg tatcttttta gtctaaataa tctctaagtt
     5641 tttttgatct acttggatgt cttagttttc ttagtgcttt tgcttctatc tgtcttattc
     5701 tctctctagt tacatcaaac tcttttccaa cttcctcaag agttctggct ctaccatcct
     5761 caagaccaaa tctaagcttt aatacttttt gttctctatc atttaacgac ccaagtacat
     5821 cttctatttg ttcttttaat aaagaatatg ctgctgcctc tgctggggct ggagcatcat
     5881 catctggaat aaagtcacct aaatggctat cttcttcttc tcctattggt gtttctaaag
     5941 acacagggtc ttgagctatt ttcattattt ctcttacttt atcttctgtc atttccattt
     6001 cttttgcaat ttcttctggt tttggatctc ttccaagttc ttgaagtagt tgtcttgata
     6061 ctcttattag cttatttata gtctctacca tatgaactgg tattcttata gttctagctt
     6121 ggtctgcaat agcacgtgtt atagcttgtc ttatccacca tgttgcataa gtactaaact
     6181 tatatccttt tgtatagtca aatttttcga ctgcttttat aagacctaaa tttccctctt
     6241 gtattaaatc caagaaaagc atacctcttc ctacatatct ttttgctata cttacaacta
     6301 gtcttaagtt tgcttcaact aacctttgtt ttgctatctc atcaccttcg tgcatccttc
     6361 tagcaaattc tacttcttca tgtggtttaa gtagaggaat ttttcctatt tcttttaagt
     6421 acattcttac agggtcatct atacttatcc cttttggtaa agataaatcc acagtttcta
     6481 tttctattgc agaagagtca tcatgtgaaa ttgcctctgc atcttcatct aagtggccta
     6541 tacttaaatc gtcatcagca accgaaaaat ctatatcagc tttatagttt tttgtttctg
     6601 ttatttctat tcccaaattt cctaaagtct cataaagatt ttctacttga tccttatcaa
     6661 gttcagtctc cgaaaaagct tccattatct ctgcaagtgt cagagaacct tgcttttttc
     6721 ctttttctat taatgtctta gcagtaacct tttttaactc ttttttattc gatttatttt
     6781 ccacactcaa acctcctttc tctacaagct ctttaaaatt ttatttatct caactatttt
     6841 taaagcaatt tccataactc taccatctac ctctttcata tcattattat tttctaattc
     6901 ttgttgttct ctcaacaagc tattaatttg ctcttctaat gagttttttc tgatatttgt
     6961 tattattcct tctatttctt ttgtgttctc taagtttata ctattcaaag aaattgaatt
     7021 taattctttt aagtattctt cggatatgtt taaacttttc aatttgtcaa tagttatttt
     7081 gtccaattct tgatttttaa tcatgtaatt taaaatttct ttactttctt tcaataaaaa
     7141 atcactttct tctactttaa gtaacgctat ttctctaatt tttttgtctt ctaacattat
     7201 ttttattaga gtttcttcaa ctaattgttt tccgtcttgt ctgacttcca ctttttcaat
     7261 tactttttct tctattttct tttgattttt gttattatat ggcttattgt aatttttacc
     7321 ataaacttct ctctttatag actcgacatt aatatctatt tgattgctta aatactttgt
     7381 ataaaaatca atttcaacag gacttgtcaa ttgttttata attttagaag cttcttttgc
     7441 gaacttcact cgttcttcat ctttttgaat gttaaattct tttttaagat gatctatttt
     7501 atattttatg tagtgagtag aaacatccat agctttttta taatcactaa gtccgtattt
     7561 tcttacaaat tcatctgggt ctttagcatc ttttaagtct aacactttta cattgatacc
     7621 taatttagta agtatatcta tagctcttaa agtcgctttt attcctgctt catcagaatc
     7681 ataagatata atagcagtgt ccgcatatct ctttattaaa atgccttgtt gttcagtcaa
     7741 ggctgttcca agggttgcta ctacattttt tataccatac tgataaagtg aaattaaatc
     7801 catatatcct tcaactaaga ctatagtttt actttctaaa ttttttctag caaaatttaa
     7861 tccgtataag ttctgttttt tattaaaaat taatgtgtct ggagaattta agtattttgg
     7921 caaagaatcg tctaatactc ttcccccaaa tcctattata tttcctctat aatcgaatat
     7981 aggaaacata actctatttc taaatttatc atagcaatta gttccatctc tattttttgc
     8041 aattagaccg cactccaata agtcttgttt tttatatcct ttactgatta gagtattcat
     8101 aagtgaattc catgaatcca aagaaaatcc taaaccaaat ttcttaataa ttttatcatc
     8161 tagtcctctt attcttaaat attcataccc aagatttttg cttcccaata agtttgaaag
     8221 ataaaatcta gctgcttccg tatgaatatc ttgaaatttc ttagattttt ctattttaat
     8281 cctagtttct tcattcatgt tagtgttaat ttctataccg catttatttg ctaaaatttt
     8341 tacagcatcc ataaaatcca aattttccat tttcatgaca aaatttatta cgtctccacc
     8401 ttcaccacaa ccaaaacact tataaatctg ttttgacgta tttatataaa aagaaggtgt
     8461 cttttcgcca tgaaatggac ataatccttt gtaattagct ccagattgtt ttatattcat
     8521 atattcagat attatacttg ctatatcgca tctagctttt atttcttcaa taatgtcttt
     8581 tatatcattc atattatcac cttctggtgt aattttttgt gttaacattt ctattctaca
     8641 ctatattcta aatttgtcaa tcactcaata tacacaattc gacttttttt attgatatcc
     8701 ttcttattaa tcaaaaaatt tttatttttt atattaattc tatcaatatt aaatctttat
     8761 ttatattaaa taatctaact atcaaattat aacttataaa tttacctcag caacgattac
     8821 gaataaaaaa gaggctcttg cctcttttaa taattattaa ttgatttcat taaatcttca
     8881 aattcattaa agcaaagaga ttgagcacca tctgacaaag cattttctgg gtcaggatgt
     8941 acttctacca ttattccatc tgctccacat gcaaaagatg ccagtgacat aggttttacc
     9001 aaatatctaa ctccagtggc atggcttggg tctaccacaa caggaagacc tgtttccttt
     9061 tgaataatgg gtactgcagc taaatctaat gtatttctag tataatcatt atacgttctt
     9121 atacctctct cacacattat aatattacta tttccttcta ttgctatata ttcagaagca
     9181 cctatccatt cagtaattgt ggatgctatt cctcttttta acataacagg tttattttgt
     9241 tttcccactt cacttaaaag agtaaagttt tgcatatttc ttgacccaat ttgaattata
     9301 tcacttatac tatacagttc gtccatatct cttacatcca taagttctgt aataacagcc
     9361 ataccaactt cgtctttaac tgcttttaat atttcaagac cttctttttt taatccttga
     9421 aatgaatttg gcgatgttct tggcttgtat gctccacctc ttaaaatgtt tgctccctga
     9481 ctttttacaa atttagcagt ctccaaaagt tgctcataac tctctattgc gcatggtcca
     9541 gctattataa gtttatcttt ttttacagtt atctttttgt tatttgtatt tatctctatc
     9601 tttgaattat tattaaatag gttttctttc ataacctaca agctccttta cttcttctct
     9661 ttcttcagca gataggaggt ttaaatgttc ttttattgtt ttttcattta ttataagttc
     9721 ttcatttaaa tccataagtg gtatcaaaac gaaagctcta tcttgaatcc ttggatgagg
     9781 tattgttagt ctttcatcag tggaaactac atcattaaaa aatagtacgt ctacatctat
     9841 agttcttggt ccccaatgaa ctattctctc tctgtgtagt tctctctcta tttcttgaca
     9901 atattctaac aactcataag gttcaaactc tgtttctatt tctacacaca tatttagaaa
     9961 atctgcttgt tctgtatatc cccatggttt tgtttcgtat aagcttgatt cttttacttt
    10021 atatatagaa tcactatttt tcaaaagttc acatgctctt gataaattat caaatctatc
    10081 acccatgtta gtacctattc ccaaataagc cttattcatc tcttgctctc cttatctcaa
    10141 caccaaaata gtcataaata ccatttacag gagcctcagg ctttctaact ctgaccataa
    10201 ctccatttat aagtataaat ttattcaaga cttcttcagc aatattttcc gctaaagcct
    10261 ccaaaagatt aaattgttta ttttcagtaa tatctttaac aacattgtaa acctcggcat
    10321 agcttacaga tttatttata tcgtcactaa gacctgcttc tcttgaatca atataaagtt
    10381 ccatgtcgac aaagaatttc tgacctagaa aattttcttc tttaagaaca ccatgatatc
    10441 catagaatcc tagattacta agtaatattt tatccattat attataccta ctttctatat
    10501 attgcatcag tcattctagc tgccattaaa ttttcataaa catcatgtac tctaactata
    10561 tctacgccat ctcttatacc aagaacagta gtagctattg taccttctaa tcttttttta
    10621 ggctcaactg gaagtacttt tcctattact gattttctgg atgtcccaag caaaatagga
    10681 taccctaaat cttttaattc atttaatctt ctcaatactt caatattttg ttcaaaatcc
    10741 ttgccaaatc ctacacctgg gtcaagtact attttttctt tttttacacc acaactcatt
    10801 gccatttcta tacttacctt aaaaaagtct tttattgact ccattatatc tttgtcatag
    10861 tttgtcccat cttgattatg cattatacac acttctgcat catattttcc tattacagaa
    10921 gccatatttt tatcttttct aagaccccaa acatcattta ccatagtgac acctaatttt
    10981 aatgcttctt ctgcaacatc tgccttataa gtgtctattg atattggtat atccaattcc
    11041 tgtttaagtt tttttattac agggattact cttcttagct cttcgtctga gtctacatag
    11101 ctatgacctg ggcgtgtaga ctctcctcct aggtctatta tatctgcacc ttggtcaacc
    11161 atatcttttg catgttgtat tgctatgtcc aagttattaa aatctcctcc atcagaaaaa
    11221 ctatctggag ttacatttaa tattcccatt atataagttc ttttaccata atcaaacaca
    11281 attaatgaca ctcccttaaa ttaaatttta tctcaataaa tgattatatc tataataaat
    11341 tgctacttca tagtaatcaa actcatagcc tcggctctag acgcaatgtc cttttcaaat
    11401 attcctctca ctgctgatgt aacagttttt gaacctggtt tttttacgcc tctcattgtc
    11461 atgcacatat gttcagcttc tactactact aagacaccat aaggttgtaa ttcttccatt
    11521 attatatctg cagcattttt agtgattctt tcttgtaatt gtggtctttt tgccaatgta
    11581 tctattactc tagcaagttt agaaagccct gtaagtcttc ctccttttgg taaataagca
    11641 atatgagctt ttccaaagaa aggaactaga tgatgctcac aacatgaata aaatggaata
    11701 tcttttacca atactaactc ttcatgtttt tcatctgcaa aaagaacttt taaatggtct
    11761 cttggttctt cactaagacc agagaatatc tcttcataca tcctagcaac tctatttgga
    11821 gtttcgatta gaccttctct atctgggtct tccccaattg cctctagtat ttctcttact
    11881 gcatgttgta tcttttcttt atcaacttta ttcatttaaa ctccccccat tgatttgtga
    11941 ctagaagatt agttttataa cctcttttgt ctcaatttct ttaaaaatat aattataaac
    12001 atctaaaagt ctttgatagt cataattttc acttactttt gacactagtt caaaaagctc
    12061 attattttct ttttttaaaa cttttagcaa ttttttgtct tttggtaccc atttatcatt
    12121 tatattaaag tacccaatta ttatatcttt taaatataaa ttcgtcaaaa aatgatattc
    12181 aaaaacatca aatttttctt tattttttaa attcgatatt tttgctccaa tatcttgctt
    12241 tataaaagac ttttcctcta atgacatttt actgggtcct tctaagtaaa tatttctact
    12301 tatatctttt ataatatgtg atatattctt cctatcaaac agtactttag catctttcat
    12361 ttctttcaca aagaaatatt ccctatttga taataatttt tttacaccat cttttgaaaa
    12421 gtaatttaca tcaaattcta ttcccttttt tttaaacagt attctctctt gtttttcccc
    12481 ttcttttaca aaaacgaaca catcaatatc gtttatctct acatcataaa gagtaaaatc
    12541 tacgtcttta ctagaaccca ctaaatatac agccaaagtg ttttggtttt ctattatttt
    12601 agttatctct tcattgtata cttcataaat acaacctatc atcttttgtc tccaacaaat
    12661 tgttaatatt ttaatttatt ctaataactt cactatttta tcaaaaacaa cattttcttt
    12721 attaaatttt attttgtcaa tttctgtaac ctttatagcg cccataagac tatttgtgac
    12781 aaaaacaaaa tcaaaactac taatttctga tatattaatt tcattttcta tcaactctat
    12841 atgtaattcg tcacaaatct ctataatcct tttttttatt attccattta atatcggtaa
    12901 tctatcacta ggagtgtaca ctctttctcc ttttataaag aaaatattgc tcatagaaca
    12961 ttctaatatt acaccttctg aatttaaaaa tataccatca ttataaccag tcttatttgc
    13021 gatatttttg gtatatatgt tttcaaaata attagtagtc ttatgtctat gaattaaact
    13081 attcccccta actattggag atatagctaa tttaaacccc ttctcatacg tctcttgatt
    13141 atatggaata tccctaatag atatattata accttcatca aaaacagtaa ttctaagtgc
    13201 tttattaatg acattttctt tttttatata tgtaacaatt tcatttatca agaaatcttt
    13261 tttatagttt atatctaaat ccaaacaatt tattgagctt agcattcttt ctatatggat
    13321 atctaaatct actgctattc catttttaac tctaatagtt tcaaacaaac caattccaaa
    13381 tttacttaaa ctactatcaa aacctacatt attcttcata atatcccctc aaagccttca
    13441 ttatggattt agctttatca agtgtttctt gatattcttc atctgggtca gaatcccaag
    13501 tcattcctcc tccaacttga aagtagactt ttttgtcatt tttgattatt gttctaattg
    13561 ctatattaaa atccatatca ccattaaaac ctatataacc tattgaacca gtatatacat
    13621 ttctttgcgt tggctctaat tcgtctataa tttccattgc tctaatctta ggtgctcctg
    13681 ttatagaccc ccctggaaaa gttgctttta ttacatcagt agcatcttta tcatctttta
    13741 actcacctac aactgtggaa actaagtgat ttacatttgc atatggttca attacaaata
    13801 gctctggaac tttaacactt ccagttttag atattctccc aatatcattt ctttctaaat
    13861 ctactatcat aagtagttca gctctatctt tttcactatt tctaagttct tgttgaagtc
    13921 ttaaatcttc ttctttatct ttccctcttg gacgagtacc ttttattggt ctagtttcta
    13981 ttctcttatt aacacatctt ataaatcttt ctggagaatt tgataatata tgtgcatctt
    14041 caaagtttaa gaatgcccca aatggtgctg ggctaaatct tcttaagtcc ctatacaatt
    14101 caaagcttgt aagttctgtt tctccactga acctttgtgt taaattggct tgatatatat
    14161 ctccttgtct tatgtactct ctaacacttt gaactgcatt tttaaattct tcctttgtaa
    14221 aattggattt aagtcttata ggtgttactt ctttttcttc ataacatatg ctatctatac
    14281 cttttttctc ttcttttaat attctttgtt ctatatcatc tattatcttt tcttctaatt
    14341 cgatatctat gtttggagta gctatataag ttttttgcac aagatggtct ataactatta
    14401 catgattata aaatccaaaa tacatatcag gcatctcaat atcatctaca gcagttctag
    14461 gtaaattctc tatgtaatta cctaaatcat aagataaata tcccacagca cctcctacaa
    14521 atggaagatt agtatcattt acaaccctat acttatgtag ttcttctttt aaaacttcta
    14581 aaggattttc atcagtatct ttatacttta ataccttaaa tggttggcta cttataaaag
    14641 aatatcttcc caatttttct ttatccatag cactgtctaa tataaaactg tcatgttcat
    14701 ttctaaaaat agtaaatatc tcaaaagaat ttaatttagt atttatctct cttatcatat
    14761 tacatatgta ccctttctct agcttctgtt ataaaatttt taagcatttc atgaccatac
    14821 tctgacaaaa tagcttctgg atgaaactgt actccttcta ttaaatattt cttatgccta
    14881 atccccatta tttccccttt atcagtaatt gctgtaattt ctaattcttt tggaactgta
    14941 ttggagtcaa ctattagtga atgataccta gtgacattta aaggattttt aagtccccta
    15001 aaaactcctt tattagtatg gttgatacta tatacttttc catgcacagg ctgttgtgct
    15061 tttataatat ctcctccaaa aacatgtcca atagtttggt gacctaaaca aattcctagt
    15121 ataggttttt tccccttaaa atgctcaaca atatctatgc aaataccagc ttcttttgga
    15181 gaacacggtc ctggagataa cacaattacc tctggattta gttcttcaat gtcacttatt
    15241 ttgatttcat tatttctttt tactacaact gtttctccta attcttctat atattggact
    15301 aggttgtata caaaagaatc ataattgtct atcataagaa tcatactgta aatctccccc
    15361 ctaaaaatta ttttatcaac tcagaaactg ttacaaattt aaaacctttt tcaatcattt
    15421 ttggaataaa catatctaaa gcttcaactg tatcattagt tgcataatca tgcaaaagta
    15481 ttatatctcc attttgtact ttattaatta ttttatctgc aatgctatta gctcctggat
    15541 ttttccaatc tttcacatct acacctgtcc ataaaacaac tttatagtca tgcttttttg
    15601 caatttctat taattctttt tctctataac ttccaaatgg tggtctaaat aatgtcggtt
    15661 tttttccagt tacttctttc aaaatgtctt cacacttaac aatctcctct tctatctgac
    15721 tagaacttaa attacttata tcaggatgat tgaaagtatg atttcctatc tcgtgacctt
    15781 ctttacttgc tcttactaat ggttctttat accattttgc atgttttcca gctataaaaa
    15841 aagtagcttt tacattgtat ttctttaata catctaaaac ttgactagtt tctttaggat
    15901 gaggaccatc atcaaaggta attgctatta gctttttttc ccttgaacca tgtttaatca
    15961 aaacatttcc attagtatcc atcatcaaag cagaaacttt actaaaatta gatattaaca
    16021 ctattccaca aaacaagact actgctatta agcacttata tactgtcttt tttttccatg
    16081 atgtcatttt ttaacttcct ttcaaatatt ttagttattt ataaaaattt tgagtgtaat
    16141 atgttttata gcttcctcca aatattactc caactccaat attattatag ttccccaaaa
    16201 tattttttct atgtccttca gagttcatcc aagcttcatg cgcatatatt gcattaatct
    16261 gtcctgctgc tatgttttct ccagctgaag tatatactat accttctctt ttcattctgt
    16321 caaatggagt ttcattcttc ttatttacat ggtcaaaaaa attattatct ctcatatctt
    16381 cactatgttt tcttgaactc aaagttgcct gttcactgta tctcaatgag tttaaccctc
    16441 tttgatttct aacactattt acaaggtcta taacttcaag ttcaaaacat tttttaagtt
    16501 cttcgctatc atctggatat aaatttttaa actcatcttc agtgctttta tcaattatta
    16561 agtatgaaca aactctattt tcttcatgaa tatcgtagaa aattgtaata tacttacctt
    16621 ccttacttat tatatcatat tgattttctg agtttattat atatctggta tttccttttc
    16681 tcttatattt taatggtgta atcttagttc tcactgtttg tctatcttga tttagtttta
    16741 tatcttgatt ttcacatgaa ttaatagaat tactataaag tgctactact tcatcatttt
    16801 caagtccaac cattgcaaat tcttttccat attgattata tacataccaa tcaaaaccat
    16861 attcactaat atcaattcta ccaggtttac caatacttga taaaacatag cttaaattat
    16921 ctcctaagct tattttttca aaattcttta tatctgactc tcctaagttc tccccttcag
    16981 tattatttga ttgtaaattt tgactttttt cttttttatc tttcttagaa ttatcattta
    17041 tatttatttt gtctaaaaat tcattccata tttcactttg aaaaaacttt tcttttaaat
    17101 catatccttc ttttaataat tcaggactat aaaataaaac aataagtact actaacacta
    17161 aagcaattaa tctcttcaat atatctctcc ttcttaaaat caatcaaaat tttttaatat
    17221 tgcatttatt aagttaatat tgatagtgta tactaatcaa tatcattcat acctatttac
    17281 agtaagtatt agaaaaaagc tttatctact aattagataa agctacagtc tcaaggaaaa
    17341 tatttcatct aatgttctat caaatataga tggcttatca aatttccaag tcccttcata
    17401 aaccaattca ttatttagat aaaaagttcc ttgcccatgt tttttaccat gcataaattc
    17461 acctatatac tttttacctt tttcccatat ataagttccc ttaccatgaa tcaaattatt
    17521 cttccattct ccttcataaa gtacttcccc tttactatta tagcaaatac cgtacccttc
    17581 catctttcca cctttaaatt cacctgcata tatacatcca tcactccact tataaatacc
    17641 ttttccatct ttttttccgt ttttccaagt tccaatatat tcatctttac tactatataa
    17701 catatttcca ataccgctca tcatatcttc ctcaaaataa cctatatatt catttccatt
    17761 tgaaaatata aagtttcctt ttccattttt tttatcttct ttaaattctc ctatgtactc
    17821 cataactgtt ccttcatcta caaaaaaagt atatgagcct ttcccctcca tctttccctc
    17881 taagaatttc cctacatata catccccatt tgaaaattta tagattttat tttttggttt
    17941 ttttgtatcg acatcatcta tatcgcgtat tgtttttcct tccaattcta atacttcatt
    18001 ttccggagca aaatcgcccc tttttaaaac acgtttcttc atgttcttta tacttctaac
    18061 ctctttgttt acttcattta tatatttcaa ataattatca tacaaattaa catattcttt
    18121 agataaattc ataatgccat tcctccaacg ttattcctag atattttact aaatctatta
    18181 aatttattat atcctaatag tggtcatgtt ttctacatat taagtaattt tttcaatttt
    18241 aattttaaaa tgatatcttt taaagtgata cacaaatcat taataaaacc tttattttcg
    18301 tatactaaat ttaaataagc tgagagatat aatatacaaa tttacattat aaactcccag
    18361 cttgtaaaat tttctataaa tcttttgctt taaaccctct tccttttact tctgtaacat
    18421 catttacaga tataaaggcc tttttatcat acttcaaagc tatatctttt atctttggta
    18481 tctcatttga tgaaactatt gtatatataa tcttcttttt ttgatgagtg tatgcacctt
    18541 ctgcatctaa aaaagttaca cctctaacta aactcttcat tatatcttct gctattaaat
    18601 cgacttcatt tgacattacc ataacagatt tttggtaatt cattgcatct ttaataaaac
    18661 tcattgaata cgcatttaga aatagaccta taagagtgta taatgctaaa tccaacccaa
    18721 ataaaaaact tcctgtaagt actatcaagc catttattgc taaagcagtg tctttcattt
    18781 ctatattctt cttaattttt agtattgcag ctattatatc tgtaccacct tgagaagacc
    18841 tagttctaaa tacaactccg actcctaaac cagataatat accaccatat acactttgaa
    18901 gcaatatatc atgtataggt ataatattac ttatttcctg agttgctcct aataataatg
    18961 aaaatacaat catatttacc aaacttgaaa tacaaaactc tttttcaagg tatatgaacc
    19021 ctaagataaa tattggaata ttgattaaaa atgttaataa acctacattt atatttgtca
    19081 aatagtttat agatgttgct atacctgtag cacctccact aagtagatgt gctggtctta
    19141 agaacccatt tattcctata gatgtcatca acatcccaaa tagcattgtt gaatatctta
    19201 aaactggatg tttttgtagg gtattcttaa cttcactcat ttctttctcc cttcaatctt
    19261 tctaaatctt tagaatttta aaagcttact cttacatata ttattcctga tgtaatagat
    19321 aatatttcaa ataaattttt tttataattt cttgaactgt catctattac atcatctatt
    19381 atattatctt tttcatatat gtctagtgca atcttttata ttctaatctt taaaaacgtt
    19441 caaatcatct catttatgcc ttaaatgaaa ctttttctag cataatatga tactgcttat
    19501 tatttaactt gcaatatagt aaatttatag tatttttttc taaatatcta ttttcgcagt
    19561 aatttatttt attttagcaa attcaagttt agtaattgtc tctttataat taattcctaa
    19621 atgaaatttt tctttttata atttcagtac atgatttttt tatttcatta tttgcatttt
    19681 tttcgttcag tttttatttt aaattttttt tattttattt tgttttaagt attcaaggta
    19741 ctcgtcttaa ttttattttt tatataaaat tcattataat tggaattagt attactgtaa
    19801 ttgtaccaga gactccaata gaaacaccac tcatagctcc ctctatttct ccaagttcaa
    19861 aagcctttgt agttcctaaa gcatgtgcag atgttccaag agcaattcct ttagcaactg
    19921 gatgatttat attccccagt ttgaatactg tagaagcaac tataccacca aaaatacctg
    19981 taaaaattat ggctacaact gttattgatt ctataccatt taaagaattt gctaaagata
    20041 ttcccattgg tgttgttata gattttggaa ttagtgaatt tattatactt acatctgaat
    20101 tagctatttt gccaattatc aatacagata caaaagaaat cactatacca caacctattc
    20161 ctacaataat ctcaaacata tgctttttaa ataattcaaa ttgtttataa agtggaactg
    20221 caagtactac tgtgacagga cctaaaaaga aatttagact atcagctcct atcttgtaat
    20281 tctcataagg gattttagat atgcttaaaa acaatattat tcctacgatt gcaattaata
    20341 aagggttaca tataggatta cttgtttttc gttgaatgta tcttcctaaa ttgaaaaaaa
    20401 ctattgttac tattatacca aatactggtg tctgtaatac attatacatt tttttgtcct
    20461 tcttccttct tttttatcat tttctccaca ataattccag tagtatacat tactattagt
    20521 gtacttaaac atatgactaa taaaagcact aatacatttt cccttattag gtctaatgac
    20581 tttataagtg atacacctgc tggaataaaa aatatagcca tattatcgag gaaaaatcca
    20641 cttacatttt ctatactact caagtctaaa actttaaatt gtagtagtag aaataataat
    20701 agcataccaa ctatactccc aggaataagt ataattcctt gtataaagga acttatatac
    20761 tctcctatta cccagataga taaaattatt actaattgat tgaaaacttt cattttccac
    20821 ctccaaaaat taaagagatt gcttcaaatc atatatttgt agttttatca acaagcaaat
    20881 atctttcttt aaaatagaaa gttataattt gacacaatct ctttttaaat ttatttttat
    20941 tttaatttaa taaaatacat acttggttgt ttcaaaaaac ttaactatat aaacctattt
    21001 ttcacttaaa atttctttta tttttgaatt gttatacata ctgtctggaa cttcacttct
    21061 caaactataa aaagtctgtt gtgcattttt taaatcgcca ttatcataat aaagcatacc
    21121 caactcgtaa tatgaatcat cataatactg gtcattttta gtataagtat ttatatattt
    21181 tttgtaatat tttatagctt catccttatt tcctaataat ttatttgtta ttgctaattg
    21241 atatattgac tcagaaatat attttttagt tttaccagaa gaaactacac ttttaaaatt
    21301 atctatggct ttttcatatt ctttgttttt ctttaaattt agagcttgat aatataaatc
    21361 ttcctcatca gctacagtta tctctctttc tggttcttta tttttaacct catttaattc
    21421 cttattcgtt ttttctaatt ttttactcgt ttctcctaac tttttatcag tttcacttaa
    21481 cttttcctct ttactactta tttggttatt caagttttct attctctttt tgctttgaat
    21541 caaatatatc cccataccta ttaaaacaca gccaactata atatatggca aaatactttt
    21601 ctttcttttt acttttttta tttcttcctt tttacttgta tcttcttttt cttcttcatt
    21661 tttttcaaat acaggattat ctttatctag ctctttaagt ttgtctaaat atccttctct
    21721 cttttttaca ttacctagtt tatcatataa caaagataaa attacatatg gctctattag
    21781 ttcaggttct tcttcaatta tgttctctaa aatatgtatt gactcataat ttaattcttc
    21841 atttataaat cttatagaat gattgtatct tcctaaaaaa gttttaaaat cttcagatga
    21901 aattagattt acatattttc tagatatatg attattttca taacataaag cagtataaaa
    21961 actctcaaat gatttagcaa aatcacattt taataatttt aaaagacctc taagatttag
    22021 tatatcaatg tctttaggat ttatctccag agctttatta accatattaa tggcactagt
    22081 tatataattt ttatatgcta aatccaatgc attgttatat cttttatatg attctaattt
    22141 taactcctta gacgttgtat ctgactttga atttaaagct ccttgcaagt ttaatagagt
    22201 ttcttcttta taatttgtag tcacataact ctgttttaat gcttttaatg tttcatcacc
    22261 gtcttcttct tctatattaa aaaaagtcaa atcactttta ttatcttcta tcaaagatat
    22321 atctaaaatt tttctaggca tgctttctat gtcttgtttt atatctttaa ctaaaataat
    22381 actcacctcc actctaaaat atatatttaa gataacatat ttaatatgtc aagtcaaaac
    22441 tatacatatt tttccaccat tctcataata aaaaaggtaa tcctcttctt ttcataaaaa
    22501 ggttacctct ttttcaatac tattaacttt taatgttgaa tttttttact tttctataga
    22561 gtgtagctat acctatattt aatgcctcag ctactttttt cataccttct gtattacttc
    22621 catatttctt tatggcattt tctatagctt ccctctcaag gtcatctata gtcttaactt
    22681 cttgtttata aagttcatct agtttaaaag atattaaatc agacatactc cttacaaaag
    22741 cagttaactc cttgtctttt gaaagtatct tttctttttg ttcttcatta aacgcagcta
    22801 tacccaagat tccaagtata tgctctccca ttttaacggg agtacatata tcagctaact
    22861 ctttacaatt ttctctattt gcacatttat tacagttctc ttctatttct ttatttataa
    22921 cataggattt tccatctttt aaaacaagag agtacactga cttctctgat attgtctctc
    22981 ctattttacc tctgaatatt cctgtacctg ctatcctagt taaatctttt gtaactaaag
    23041 taacatctac atccaagaca ttagaaatat tctcgcaaat actttgaacg taattcgtta
    23101 tattatttaa attcaaagta atccctccta tttagattta tattttttat tactctacat
    23161 aaatcctatt cttgagaact ttaagcttgc ggttgtggtt gtgtctcatt aggcttatta
    23221 tcagttggtg gtgtttctgg tttttgttga ttatttggat tttgattgtt tgtattttga
    23281 ttattcttat tttcgtctgt atttggtttt tcctctggtt ttggttccat agtcccaact
    23341 aatataatac cttccttctt aggataatat gaattagcta ttttttcagt tttaattttt
    23401 ttaccatttg catcattaaa tgttctatat gttgatacag aatatgcatt tcttccactt
    23461 ttgtctactt tttctttacc tttaggcatt gtagggtcat taactctttt cattgtagtt
    23521 tgtgaaacgc catctaattt tgttgatatt gttatatttt gtttatcttc tgcacttcca
    23581 tatatattgc aaacaatttg atttcctgaa acgtaatttt ttatgtaaac tggatgcttt
    23641 agattatttt taaataagaa atctattcca ctatctgcaa cagttgcatc tcttcctttt
    23701 ggaacataac ttgatggtat agtatgattt tttacattaa caatttctaa acctgcgtat
    23761 aacacagaat tgtataacgt agatgaaact tgacaaactc ctccaccata atcttcttcc
    23821 atctcacctt ccattattac aggtgcactt ttatatccat tttccttact tcttttccct
    23881 gtatgtttat taaatgagaa agtttctcct ggcataagta atacattact tatattatct
    23941 gttgctaatt ttatgtttat acttctattt atttgagaag ttttaaatgt tgttgaatac
    24001 gtacctaaat ttgtatctat attagcaagt tgctcttttt ttacttttgc tggagtagct
    24061 tttactacta actcattttt atagttacct tttttagttt gattatcaaa attctccata
    24121 ctcttagaaa tatccatttt aagaccatca gaatctggta taaccttgac tttttcacca
    24181 cttatatcaa gtgtagcatc ttttacgtct acatctattt ctttagctat cttttccatt
    24241 tctgctttta atttattttt atcataattt atcgcaacta ctaagttgct ttttttgcca
    24301 aaatttgctg aaatagtttt tgatagattg ccaaagaaag cactctttct atttaaatta
    24361 tatgcgtttt ctactgtttt atccaggtca gtagataaat ttaaatcttt gctctttact
    24421 ttccaacttt tatcattgta attaaaatca acattctcta atttgtattt tttagctaat
    24481 tcctgttttg cttgcgattt agttaatttt ccaatattta ctccattaac atatgtgttt
    24541 tttgctattt tctccccttt aaattgcatg cacattatgc ctataaaaat tattaccact
    24601 aatgctacag ctccaatagc tataactagc tttttgttca cattactcat cccatacttc
    24661 tccttacaat aaaattattt atatatccct attattatat tactacataa tgcgccttaa
    24721 aaccccaaaa actttttaat tatatattaa tttaatatta ttaatattca tttagtccat
    24781 tttctgatat ggcatatatt ttttcattgt aaatattaat agcataaact tctttgcctc
    24841 taaataagcc tttatttact acagcataat aataaatatt tttatatttt ccaaacaatt
    24901 cttctaaact ctttttactt tttacatatc tatctgcttt ctctatatct aatatctcaa
    24961 tctttttata cttattttct ttttcatcga gagtattagt taacacaaac ttagctttat
    25021 cttttgtaat accaaattga taacttccat cttctaatat tctaaagtat ccatcaccat
    25081 aatctacttt gtaatctttt ttaccatctt tatatgaata taaatcttta gtttttgcac
    25141 ttaccaaaac ttcatttatt aatttatcat actcatatac ttcagcataa tagtatttat
    25201 tattttttat catttgtgga agtgaattta tagatagatt tgccttgtct ttgccacttt
    25261 ttttagctat tacatttata atgtcatcag atgttaaagt atacaatgat aaattctttt
    25321 tatatttttt ttctaaactc tgtacctttt catcatctga ataatcgtct tttaaatact
    25381 caatatattg taatgctctt tcataatctc cagaagtatt tgcctcttca gctctactta
    25441 tatagtaatc atacatttct tcaatacatt gttctttccc attttgagcc atactgtaat
    25501 acttttcatc ttcttttaat actttattat acttttcaat agcttcataa tacttatatg
    25561 ctttttggtc ttttactgct gagtcataaa catctctaga ctctttgatt gtttctatat
    25621 tctgtttgta tacatctagg ttactaccca ttccatttaa tattgaaaca gtacttatgt
    25681 agttaatggc aatatcataa gtagtatttt cttctttata catttctcta actctatcag
    25741 cttgagctaa taagtcatcc acatctatac ttattttgtt taattctttc acggtattta
    25801 ttaatccaat aaaactttct tgacttatgt ctccatctaa atatttatca ccactattta
    25861 atagcaattt atttattttt ttagaaacag acttattaaa cctttccatt tttgaatcag
    25921 aaaacttttt ttctatactt gtgtagtaat caacagctat agaatactgt cttttctcta
    25981 taatctttaa caactcatta gcttcatttc ctacactaaa ctcgttacta cttaagaaaa
    26041 atactattat caaagcaatt acacctaatg ttgataatga aatcgcaact atagatttcc
    26101 ttttttcatc aataaaatct acagccttct caaataaatc tctaaaattc attatagttc
    26161 cctctccatt taataatctt tatattttat ggtaaactaa atttcttatt ggttattata
    26221 tcataattct tgaaaaaaaa taaaaacaca agttaaatcg tgtttttatt ttacctatca
    26281 atcattattt tatacataaa tatttccttt acataaatta tgatatcttt ctttttatat
    26341 ccctagaatg actcttttgt tttttattat tactgacagt tttcgttctt gtcttgtgta
    26401 tagctttgct atcaaatatt tttctagcat tgcataaaac aattccaatt gttaaacaaa
    26461 ctgtaagcac acttcccatc aaaaagtaat aatccaatcc tatatgtact cccataactg
    26521 aatttattaa cactaatgca aaagtcattg caaaacatat catcatggag ataactaaaa
    26581 ttcttagaat ttttttcaat ccactcacct cgtataatca ctaaaattga cactacccct
    26641 tatccattac tataccaaat tagtaaaaaa atagcaatta ctattgtcat ttatattagg
    26701 aaaatattaa atctttttaa taaatatctt tttttgacat aaggattact taacttttaa
    26761 taaaatagta acactaatat gtattaatta atagaataaa ataaatgata atttattgag
    26821 gggtgattta catgccaaat ttgccaagtt tagggtcaaa ggctcctgat tttaaagcca
    26881 atacaacaaa tggtcctatt agactctctg actataaggg taattggatt gttttatttt
    26941 cacatcctgg tgattttaca ccagtttgta ctacagaatt tttatgtttt gctaaatatt
    27001 atgacgaatt taaaaaaaga aatacagaac taattggtct aagtgttgat agtaacagtt
    27061 cacatttagc ttggatgtat aatatttctt tacttacagg tgtagaaatt ccatttccta
    27121 ttatagaaga tagagatatg agaattgcca agttatacgg catgatatca aaaccaatga
    27181 gtgatacatc aactgttcgc tctgtattta ttatagataa taatcaaatt ctaagaacga
    27241 ttctttatta tccactaact acaggaagaa atattccaga aatacttaga atagtagatg
    27301 cacttcagac tagtgataga gataatatag ttactcctgc aaactggttt cctggaatgc
    27361 cagtgatttt accttatcct aaaaactata aggaattaaa aaatagagtt aacagttgta
    27421 ataagaaata ttcatgtatg gactggtact tatgttttgt accagataat tataatgatg
    27481 aagaagtgag caagaaaatt gataatactt gtagctggaa aaaagaacat actaaaaaca
    27541 ttgaaaatga atgtaactat gaacatgaac atcatgacta cctgaacaaa gctcttgact
    27601 gtaaacaaga acacaagact gatattaaag atgattgcaa tcatgagaaa aaacatacta
    27661 aaaatactaa caaagttcac aactccaaac aagataagtt taaagataag tcttgtgatg
    27721 aaatgaattt taactatgac aaagatgaat cttgcgacaa aataaattct agctataaca
    27781 aagaagatag tagttatgaa gatttctata aacataatta taaaaactac gattatacta
    27841 gcgaaaaaaa tactaaaaaa atagctatga aaactttaaa agattctaaa aaattagtta
    27901 gaccacaaat aacagaccca tacaatccaa tagttgaaaa tgcaaactgt ccagatataa
    27961 atccaattgt agcagaatat gttcttggaa atccaactaa tgtagatgct caactattag
    28021 atgcagttat atttgctttt gctgagatag accagtctgg aaatttgttt attccttatc
    28081 ctagattttt aaatcaatta cttgctctta aaggtgaaaa acctagctta aaagtaattg
    28141 tagctattgg aggttgggga gctgaaggat tctctgatgc agcattaaca cctacatcta
    28201 gatataattt tgcaagacag gtcaatcaga tgataaatga atatgcttta gatggaatag
    28261 atatagactg ggaatatcct ggaagtagtg catctggaat aacatcaaga cctcaagata
    28321 gagaaaactt tacactctta ctaactgcca taagagatgt tataggagat gataaatggc
    28381 ttagtgtagc tggaacagga gatagaggat atataaattc aagtgctgaa atagataaaa
    28441 tagctcctat aatagattat tttaatctta tgagttatga ttttacagca ggtgaaacag
    28501 gcccaaatgg tagaaaacat caagcaaatc tttttgattc agacttatct ttgccaggat
    28561 atagtgttga tgcaatggtg agaaatcttg agaatgctgg aatgccttct gaaaaaatcc
    28621 ttcttggtat accattttat ggaagattag gtgctactat aacaagaact tatgatgagc
    28681 ttagaaggga ttatataaat aaaaatggat atgaatatag atttgataat actgctcaag
    28741 ttccgtattt agttaaggat ggagattttg caatgtcata tgatgatgct ttatcaatat
    28801 tcttaaaaac tcaatatgtt cttagaaatt gtctaggtgg tgtattctca tggacatcaa
    28861 cttatgacca agcaaatata ctggctagaa ccatgtctat tggtataaat gaccctgaag
    28921 tattaaaaga agaacttgaa ggtatttatg ggcaattcta atagaatgta ggaaaatttt
    28981 atagttatat aaaaagcaga ttttattata ttaaataagt tgtatctaat taatactatt
    29041 attaattgtt tataaattag atacttatt
//
LOCUS       DAELJO010000027        28899 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELJO010000027 DAELJO010000000
VERSION     DAELJO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.027"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            30..476
                     /gene="lspA"
                     /locus_tag="KPB88_001083"
     CDS             30..476
                     /gene="lspA"
                     /locus_tag="KPB88_001083"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0053381.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            445..1392
                     /locus_tag="KPB88_001084"
     CDS             445..1392
                     /locus_tag="KPB88_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0053382.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDTVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            1752..2285
                     /gene="pyrR"
                     /locus_tag="KPB88_001085"
     CDS             1752..2285
                     /gene="pyrR"
                     /locus_tag="KPB88_001085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG0053383.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            2569..3780
                     /locus_tag="KPB88_001086"
     CDS             2569..3780
                     /locus_tag="KPB88_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG0053384.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            4079..5731
                     /gene="abc-f"
                     /locus_tag="KPB88_001087"
     CDS             4079..5731
                     /gene="abc-f"
                     /locus_tag="KPB88_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG0053385.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVCKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENRIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(5946..7721)
                     /locus_tag="KPB88_001088"
     CDS             complement(5946..7721)
                     /locus_tag="KPB88_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG0053386.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSRFLGISPIIAKEICYRAG
                     VNQNAIIKYISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            8017..9051
                     /gene="ltaE"
                     /locus_tag="KPB88_001089"
     CDS             8017..9051
                     /gene="ltaE"
                     /locus_tag="KPB88_001089"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG0053387.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            9338..10168
                     /locus_tag="KPB88_001090"
     CDS             9338..10168
                     /locus_tag="KPB88_001090"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG0053388.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            10172..11053
                     /locus_tag="KPB88_001091"
     CDS             10172..11053
                     /locus_tag="KPB88_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG0053389.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            11083..11700
                     /gene="gmk"
                     /locus_tag="KPB88_001092"
     CDS             11083..11700
                     /gene="gmk"
                     /locus_tag="KPB88_001092"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG0053390.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            11703..11969
                     /locus_tag="KPB88_001093"
     CDS             11703..11969
                     /locus_tag="KPB88_001093"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG0053391.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            11974..13173
                     /gene="coaBC"
                     /locus_tag="KPB88_001094"
     CDS             11974..13173
                     /gene="coaBC"
                     /locus_tag="KPB88_001094"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG0053392.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            13412..15937
                     /gene="priA"
                     /locus_tag="KPB88_001095"
     CDS             13412..15937
                     /gene="priA"
                     /locus_tag="KPB88_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG0053393.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            15955..16395
                     /gene="def"
                     /locus_tag="KPB88_001096"
     CDS             15955..16395
                     /gene="def"
                     /locus_tag="KPB88_001096"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0053394.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            16410..17339
                     /locus_tag="KPB88_001097"
     CDS             16410..17339
                     /locus_tag="KPB88_001097"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG0053395.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNVPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            17349..18101
                     /locus_tag="KPB88_001098"
     CDS             17349..18101
                     /locus_tag="KPB88_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG0053396.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            18170..18871
                     /locus_tag="KPB88_001099"
     CDS             18170..18871
                     /locus_tag="KPB88_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG0053397.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            18933..20258
                     /gene="rsmB"
                     /locus_tag="KPB88_001100"
     CDS             18933..20258
                     /gene="rsmB"
                     /locus_tag="KPB88_001100"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG0053398.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            20266..21297
                     /gene="rlmN"
                     /locus_tag="KPB88_001101"
     CDS             20266..21297
                     /gene="rlmN"
                     /locus_tag="KPB88_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG0053399.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            21317..22069
                     /locus_tag="KPB88_001102"
     CDS             21317..22069
                     /locus_tag="KPB88_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG0053400.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            22086..24101
                     /gene="pknB"
                     /locus_tag="KPB88_001103"
     CDS             22086..24101
                     /gene="pknB"
                     /locus_tag="KPB88_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG0053401.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            24209..24946
                     /locus_tag="KPB88_001104"
     CDS             24209..24946
                     /locus_tag="KPB88_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0053402.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            25044..25928
                     /gene="rsgA"
                     /locus_tag="KPB88_001105"
     CDS             25044..25928
                     /gene="rsgA"
                     /locus_tag="KPB88_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0053403.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            25933..26604
                     /locus_tag="KPB88_001106"
     CDS             25933..26604
                     /locus_tag="KPB88_001106"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0053404.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            26613..27254
                     /locus_tag="KPB88_001107"
     CDS             26613..27254
                     /locus_tag="KPB88_001107"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG0053405.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            27385..28014
                     /locus_tag="KPB88_001108"
     CDS             27385..28014
                     /locus_tag="KPB88_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0053406.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            28069..28725
                     /locus_tag="KPB88_001109"
     CDS             28069..28725
                     /locus_tag="KPB88_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0053407.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        11492 a         2912 c         5365 g         9130 t
ORIGIN      
        1 ttttaaataa tttatagggg gaaaaatcat tgctatatat attaataata attctactca
       61 taggtttaga ccaactgtct aaaatatggg tattgaataa tttggtggat gtatcaacaa
      121 taccaataat aaataatgta tttcatttaa cttatgtcga aaatagaggt gcagcatttg
      181 gattattaca aaataatcaa tggatattta taattgttgc attacttgca acagtatttg
      241 gactatacta tcttaataca aggaaagtac atatatttgg aaggttggga attatattaa
      301 ttatatctgg tgcattggga aatctaattg atagagtacg attaggtttt gtagtagatt
      361 acttcgactt tagaattata tgggaatatg tattcaatat agctgatgta tttgtagttg
      421 taggaactgt gtttttatgt atatatgttt tattttttga aagtaaaagt aggtgatata
      481 atggatgaag taaaagagtt tttagtactt gaagaggaag aagaagttag acttgatatt
      541 tatctagcag aacaattagg tgatatgtca agaagttata tacaaaaaat tataaaagat
      601 ggaaaagtaa aggttaataa taaagtagaa aaagctagat atctggttaa agaggaagat
      661 aatattgtta tagagatacc agaacctaaa gtattagaag ttttacctca agatattcca
      721 atagatattg tatatgaaga tgatgatgtc ttaataataa ataaacctca ggatacggtt
      781 gtccatcctg ctcctggaaa ctatgaaaat acgttagtaa acgggatttt atatcattgt
      841 aaagataagt tgtcatcaat aaatggagtt attagacctg gtatagttca tagaatagac
      901 aaagatactt ctggtcttct tatgatagct aagaacaatt atgcacataa ttttctagca
      961 gaacagttaa aggaacatac tataactagg gagtatgaat ttatatgcta tggtgtggtt
     1021 aaagaagata aaataactgt tgataaacct atagggagaa atcctaagga tagattaaaa
     1081 atggctgtag taaaagatgg aaaaaatgca atcactcact ttgaagttgt agaaagattt
     1141 gataagttta cacatatgag agctaggctt gaaactggta gaactcatca aataagagtt
     1201 catgctttgt ctataaatca cccactttta ggtgattatg tatatggtcc aaaagaaact
     1261 aaatttaaat taaaaggaca aactttgcac gctaagaagt tgggatttat acatcctact
     1321 actaaggaat atatcgaatt taattcagaa ttacctgagt attttaagga agttattaaa
     1381 aagataaaat aaccatttgt ataatactta taaatcattt tttgttttta tataagtaaa
     1441 taaatttagt gaaatttaaa taaaatatct tatattaaag tattttgttt aaaaaagctt
     1501 gaaaaatttg aaatataaat atataatttg atttgataga ttaaaaaatt atctttaaac
     1561 gatacagaga tatcgtaaga caattgctaa attgcactgt atatttttta tataagagct
     1621 tcttgtcttg tgatgagaag tttttttgtt gtttaaattg aaattttaaa taaaaataaa
     1681 tttataaaaa attaaaagaa aggagtagat ttgtcaaata gatatattat atctttgaca
     1741 ataattatga tatggtagaa aaagctcaat taatggatga aaaggcaata gcaagagcaa
     1801 ttactagaat aagtcatgaa ataattgaaa gaaataaagg tgtagaaaat ctagttttag
     1861 ttggaataaa gactagaggt gtgccaattg caaatagaat ttctaagaaa atagaacaaa
     1921 tagaaggaac aaaagtagat acaggagata ttgatataac tttgtataga gatgatttgg
     1981 aaaaaattca tgttgaacct gtagttaaag gaacttattt agattttaat gtaaatgata
     2041 agacagtagt tttagttgat gatgttttat acactggaag aactgtgaga gcttcactag
     2101 atgctattat agatatagga agacctaaat caatacaatt agcagtgcta gtggacagag
     2161 ggcatagaga gttgcctata agagcagatt atgtaggaaa aaatgtacct acttctagac
     2221 atgaaattat atctgtcagt ttattagaaa ttgatggaga agattcagtt acaatcaaag
     2281 aataattatt tttaagtatt atataaaagc ttagcattga atagtcaggg aggaggataa
     2341 agcttacata gattgcacaa aataaatatt ataattttat ggatgtgagt cttaatgtag
     2401 tttttattct cttttatttg agtttttgat gtcgtaaaat gtaaaataat gataaattat
     2461 ataataagta tatagaggtt aaaaaataga atctagggaa tgataattaa ttaaaagata
     2521 ttaattaaac agaggattta tttatatata ataattttgg aggtttttat gaaaaaaacg
     2581 ataatgtctt tacaacatct attagccatg tttggagcga cagtattagt tcccattttg
     2641 acaggtttta atccgtcagt tgcaattttc tgtgctggtg ttggtacatt gatatttcat
     2701 ttttgtacag aaggaaaagt acctgccttt ttaggttcaa gttttgcgtt tataccagtt
     2761 atactagcag ctaaagaggc gtatggtgga gatttagcat atgctcaagg tggtataata
     2821 gtagcaggat taatttatat tataatgtcg attatagtaa aagtagttgg tgtaaataaa
     2881 attaaattat attttcctgc acaagttaca ggagctatga tagtagttat aggattaaat
     2941 ttattgccaa cagcttttag tatggcttca gccaattttg tgatagcatt tataacttta
     3001 gcaatagcaa ttttaactaa taagtttggt agaggattta taaaacaatt aggaatatta
     3061 atagcagttt tttctgggta tataatctgt ttgatattaa gattagtaga tataactact
     3121 attacagaag caagtttatt tgcaatacct aactttacag ttcctaagtt cagtttagga
     3181 gcaattgtga ttatatctcc agttgtatta gctgtattta tggaacatat aggagatatg
     3241 actactaatg gtgctgtagt aggtaaaaac tttatagaaa atcccggatt aaatagaaca
     3301 ttacttgggg atggatttgc gactatagtt gcagggtgtt taggaggacc agccaataca
     3361 acttatggag aaaatacagc agtacttgct ataactaaaa actatgaccc ttcaatactt
     3421 agacgtacag caatatttgc tatattgctt gcatgtgtag gcaagtttgg tggatttctt
     3481 caaagtatac caggttcagt tatgggtgga attagtataa tgctattttc gatgataact
     3541 tatgtaggat taaaaactat aagggacagt tcttgtgtag aaagtaaaat aaatatatta
     3601 atcatagcag ttatattatt aataggtctt ggaacaacat atttatctaa taaaggtatt
     3661 tctgttggaa tccctataac aagtacagta aagataactg gtcttagttt agctgcaatt
     3721 gttggaattg tattaaacag aattttaaat aatcaggact ttaaagttga agaagaataa
     3781 atttaaagaa taagttgtat attgaataac taaaattagt taattttcta tagacatttg
     3841 taattaatgt tgatataatg attaaaaata aatgaaagaa agggcaaggt aactaggaca
     3901 atgagaatat aatctatttt ttataattta aagtattgta atttttgaaa agcctatttt
     3961 ggtggcttta tttgcaatgc ttaaattaag gagagtagat tatagtctat acgtttattt
     4021 gttatgccta tttatagaat ctctccttat atttaagtgt gttaaggagg gattttttat
     4081 gttgttagta aaagtagaga atttaaagaa atattatgca gataaattaa ttttggatat
     4141 agataaactt gaaatattag aaaatgacaa aataggtcta gttggttcaa atggtcaagg
     4201 aaaaacaaca ttattaaaag caatattggg agaaatagaa attgatgaag gatatactta
     4261 ccttacagaa agctattctt atataagtca gagtgaaaat aatattgaaa catgtagtca
     4321 tagtaaggag aagagtcttt taaatgctcc agataaattt gaagaacact tatcaggagg
     4381 ggaaaaggtt aagttaaaaa tagcagatgc gctgagtaat aagaaaaata tcataatagc
     4441 tgatgagcca acttcaaact tagataaaaa aagcattggg gttttggaag atatgtttaa
     4501 aaggcatgag ggagcattat tattaatatc tcatgataga cgttttctgg atgagttatg
     4561 tacaactata ttagaactag aagatggaaa actaaaagct tacaaaggca attacactga
     4621 ttatttaatg caaaaagatg aagaagtaaa aagagctgat tttgagtatc aagaatatgt
     4681 taaagagaaa aaaaggcttg aaaaagctct tttatataaa aaagctttaa gtgatggcat
     4741 aagaaaaaca ccaaaaagaa tgggtaattc agaagctagg ttgcacaaaa tgggtggtca
     4801 gactaataag aaaaagttgg actcaaatgt gaaggctata aaaagcagaa ttgataaact
     4861 tgaggtaaag aataaaccta aagtttctaa agaaatgaac attaagatgc aagatggtat
     4921 ggagataatt agcaaaaatc tagtagaggt aaaagatatg actctaaagt tagaaaataa
     4981 gctcctgtta gataatgttt cctttaagat aaaaagaggt aaaaagatag cattgttagg
     5041 tgataatggg tgtggaaaaa gtactctgat aaaagaaata ttggctgata aaaatgacaa
     5101 tataaaaata aataacaagg taaaagtagg ttattttgac caaaatcaaa gtttattaga
     5161 tgaggaaaag agtgttctat acaatactaa agttaatagt tcatttgatg aatcttttat
     5221 aaggataaac ttaagcctat ttgggtttaa aggtgatgat gtctgcaaaa aagtaaaagt
     5281 acttagtggt ggagaaaagg taaaaatagc actatgtaaa ataatattgg aagataataa
     5341 ttttttagta tttgatgaac caacaaatta cttagatata aaatctatgg aagcactgga
     5401 aaaggcactg ataaatactg ataaaactat gcttatagtg tctcatgata gagtatttgt
     5461 atcacatatt tgtaactata ttatagagat aaaagatgct aaaataaggg agtttgattg
     5521 taattatgac gaatatacaa ttagcagaaa taaaaaaact cctagtagag aaaaccgaat
     5581 caaaaaagaa aatcttctag tattagaaaa tagacttaca actgttatat ctatgttatc
     5641 tatagaaaaa gataatctaa aaaaagaatt gtatgaatca gaatataatg aattactaaa
     5701 acaaatcaat aaattaaaaa atagctttta gtgttaactt tttaattaca tctatataag
     5761 aagactgaaa atatttgttg ggaatataag tatagttatt aaaacagaaa agtattttgt
     5821 tagaaaatta aaagtaagct aattagaaag tggccttatt attgtacttt ataagaaggc
     5881 cactattttt atttagaatg ataatgtgta gttgtctaac atttattttg tatttagtgc
     5941 agaatttatt cagatttaac cttaagcttg gctactgttt cttcttcagg agtaacataa
     6001 atagtactgt tagtttcata aataaccata cctggtttag aaccactagg ttttttcaca
     6061 ttttttctct tagtatagtc tacaggcacc tgagaagaaa gttttgactt actaaagaat
     6121 gctgctagca tagctccttc aaatacagta ttatcaggga cttccttacc agcacacttt
     6181 attattacat gagaaccagg aatattcttt gtatgcatcc ataagtcatc attatcagca
     6241 agtcttagtg taagatagtc attttgctta ttgtttttac caaccaatat tttaaatcca
     6301 tcagaagaca caaattcatg aggttttgta gatgggatag tatccttttt actgtttttc
     6361 ttttgagtct tgatgtatcc aactttagca agttcttctt ttatatcttg taattcagct
     6421 aaattttcac agttttcaat acttaaaatt atgttttcta agtaatctat ttcttcctta
     6481 tttaaggaaa tttgatgaga tatctcgact ttagcatgtt tcattttgtt atacttttta
     6541 aagtattttt gagcattttc agatggtgtc aagtttttat ttaattctat tgttacatca
     6601 ttacaatttt catcatagaa gttagcaact tcaatacttt ccataccttt ttctaccata
     6661 taaatatatg aagtaatcaa ttctcctttt atcttataaa tgtctgcatt ttcggattct
     6721 gataattctt cttcttgttt tttaagttta ttataaagtc tatcaagctt aacagatata
     6781 ctcttcttta aatcagatga acgttgatta attctatctt taatatcctt agttctatag
     6841 aaatcttcca gaatacgaga catactatcc ttatttatat atgataaatc gctaaataat
     6901 gtaaggttta tacaactgaa atcaactacc ttatccacct ttttatctat aataatgcaa
     6961 ggagagtatt tgttagaatt tatatcattg aataaattac aaaatacttt gtgtaaagaa
     7021 tcaaattgtt catcagatat atactttatg atagcatttt gatttacacc tgctctgtag
     7081 caaatttctt tagctataat agggcttata cctagaaatc tagaatatat agacttaaag
     7141 ataggtcctt ccaattcctc taggttttta ataaacaaat ccttagatat atcatcaaga
     7201 gggttgagtt tgttttgttc tggtggtaat gaataatcat gaccaggtaa aacttgacga
     7261 actctactta tgctaaaagg gactcttttt atagaatcta taattttatt ttcttcacca
     7321 tgagttatta ttatgttgct atgtcttccc ataatttcaa tcataatatt tttaactgtc
     7381 ttttctttta attcatctaa agattcaaca cttattttta ttattctttc aaaacctatt
     7441 tgagaaattt ctactatatt tccaccttgt atgtattttc taagtaacat acaaaacata
     7501 ggcgctttta gagggttttc tttttgataa ttacttgtaa gataaactct aggatttgag
     7561 gcacttgcac ttaaaaccaa tctaaaattt tctttattat ttcttatatt aaaaattacc
     7621 tcatcatctt caggttgatg tatcttatct atttttccac ctgtaagttt tgatgataat
     7681 tcatcgacta ttgaatgtat aactaatcca tctaatgcca tttatagcac ccctttcttt
     7741 gaccaaaatg agtcagtaaa ttaattatac acataatata cgctttgtga ctagggggaa
     7801 tttaaacaat agagattaat atttaaattg tatgattatt tatatttatt agagaggaaa
     7861 ctttattttt aaaattaata atttaaagta gattaaatat aaagtaaata tagtataatt
     7921 attgtgtaga gtataagcta tgaaagtata tgaactttac agaaaaaatc aattttaagg
     7981 ttctatagta tataaatatg aaagaaggtt gaaaaaatga gatttatcga tttaagaagt
     8041 gatactgtta ctatgcctac tgacgaaatg agagaagcta tggcaaaggc tcctgttgga
     8101 gattcaatct ttagagatga ccctactgta aataaacttg aagaattagc agcagctaaa
     8161 gttggaaaag aagatgctat tttccttcca agtggaactt ttgggaatca attagctcta
     8221 tttactcatt gcctaagagg gcaagaaata attattggta aaggttatca cattgtgact
     8281 catgaggttg gggcacctgc tgtaatagct ggagttcagc ttagaactat agatgaagac
     8341 gaaagtggag ctctaaatcc tgaacttgtt gaaaaggcaa ttagaagaga tgacatacat
     8401 gaaccacaga cagggcttat atgtgtagaa aatgcatatt caggtggaac agttgtgagt
     8461 cttgataatt taagagaaat taaaaaaata gctgagaaac ataacttacc agtacattta
     8521 gatggagcaa gattatttaa cgcagcatta aatttgggtg tagaagctaa agaaatagca
     8581 aagtgttgtg atagtgttat gttctgtgtg tcaaaaggtt tagctgcacc aatgggttct
     8641 atacttgctg gaagtaaaga atttatagca aaagctagaa gaaagcaaaa agttatgggt
     8701 gggggaatga gacaagtagg tatagttgca gcagctggta taattgccat tgaaaaaatg
     8761 actttaagat tgaatgaaga ccatgaaaat gcaaaatatt tagcttgtga attaaataaa
     8821 attgatggca ttgaagtaaa taatgttaat ccagatataa gtatggtatt ttttaagatg
     8881 agtgaagata ttataaaaga ggatgtttta ataaaagaat tttttgaaag aaatataaaa
     8941 attaataaaa tggaaaatgg tgaatataga tttgtaactc atgtagatat tactaaggaa
     9001 gatttagact atgttttaaa aacacttaaa gaattaatag gtgtatgtta atagatatct
     9061 actagaagga ttatagctag ttaccagatg tttaattaaa aaattcatag acagtttaaa
     9121 taaaacatac ataaaattca tatagaaata ttaatatata aacatagaaa gaactagctt
     9181 aaaaacataa tataattacc ctaattgtaa tggcaactct ccctcacatc ctcaaattat
     9241 agttgtcatt acaaaacttt tcccataaat tataagttta tgggaatttt tttatgttat
     9301 aatagtttaa gcgttaattt acgaaggagc gatacttatg aaattttgga aactacatgg
     9361 aataggaaat gattttatag caatagatgg aagattcgac cagataaatc catctgatta
     9421 tactgcttta gcaaaaaaag tatgccatag aagattttct gtaggagcgg atggattgtt
     9481 agttgtgaaa gattcaaaag ttgcagatgt agaaatggta tattataatt ctgatggttc
     9541 aagagcagct atgtgtggaa atggccttag atgtttttgt aaatttgttt atgataattg
     9601 tatagttaaa aaagaaagtt ttacagtaga cacattagat ggtataaaag atataaaagt
     9661 aaatcttacc aatagagaaa taaactctat aagagtcaat atgggaaaag gaagttttat
     9721 agctaaggac gttcctgtac ttacaaataa agaacgtttt gtgcaagaaa gtataaaggt
     9781 tttagatagg gagcttaaag ttacttctat gttgatgggg gttcctcata cagtagttat
     9841 agtagatgaa ttagatgtag atgatgtttg taaatatgga aaagagattg aaaataataa
     9901 aatatttcct gaaaaaacga atgtaaattt tgtaaaggta gaagataagt ataatataca
     9961 tgtttatact tgggaaagag gttgtggata tactttaggt tgtggtactg gtatgactgc
    10021 atcagcaata gtgtgcaatt tacttaatat ggtagaaagt agtgtaaatg taacgtctca
    10081 aggtggaaca gttaaaatag atgtaggaga tttctcttat atgacaggtc cagcagtaaa
    10141 aatatgtgaa ggtattttgg aggtttaatt aatggctata agtatgactg gttttggaag
    10201 aggagaatac aaggatgata attattactt tttagtagag tgtaaaacga taaatcataa
    10261 gtattcagat ataaacatac gtcttccaag aaaaatttca tttttagagg ataaagttag
    10321 aaatttagta aaaaattatg taaaaagagg aagagtagat ttatacataa agtttgactt
    10381 attaggcaaa gaagatgtaa acttaaattt tgatgaggga ttagcatctc agtatataga
    10441 catactaaaa gaaataaaaa ataaatttga tattatagat gatattagcg ttatgaatgt
    10501 tgcaaagttt cctgatattg tgaaaataga agaaaaggaa gaagatgaag atttattgtg
    10561 gtcaatgttg aatcaagcag ttgaagatgc attaatcaaa ttaagagaaa tgagaagtga
    10621 agaaggtaaa aaattagctg aagatattgc catgagatgt gatttattaa aaaatcatat
    10681 agaagagatt gaaaaatatt caagtagtgt tgtagaagat tatagagaaa aattgaattt
    10741 gagaataagt gaattactgg atgacccaag tataatagat gagaatagat tagcacaaga
    10801 agttgcaata tatgcagata aaagcagtat aacagaagaa atagttagat ttaaaagtca
    10861 tatagggcaa cttaaaaata ctatatttaa agacgactct ataggtagaa aaatagattt
    10921 cttaatacaa gagatgaata gagaaactaa cactataggt tcgaagtcat ctgatataaa
    10981 tataactaat ttagtagtag aagtaaaaag cgaattggaa aaaataagag aacaaataca
    11041 aaatatagag taagaataca atattagaag gggaatgctt aaatgtttgt gaaaaaagga
    11101 ttattgctag ttgtatcagg accatcaggt gcaggaaaag gtactatttg caaagaacta
    11161 cttaaagaaa atgatacaat aaaactttct gtatcagcta ctacaagaaa acccagaact
    11221 ggagaagttg atggagtaaa ttatttcttc ataagtaagg aaaagtttga agagatgata
    11281 gaaaaaggtg agtttttgga gtatgctcaa atttatgata atttttatgg aactccaaaa
    11341 gcggcaataa tggaatgttt agaaaaagga caagatgtat tactggagat tgaaatgcaa
    11401 ggagcaaaac aaataaaaga agtttgccca gagggagtat ttatatttgt actaccacca
    11461 tcacttgaag aattaaaaaa tagaattgtt ggaagaggta cagaaacaga agctgaaata
    11521 gagaagagat ttagctgtgc atatgaagaa attaaaatga ttaaggatta tgattatttt
    11581 atatttaatg aagatgtaaa aacatctgct aaagaaatag aaggtataat atcatctgaa
    11641 aagaataaag ttagcagata taaaaatatt ataatagaga aatttaagga ggaattataa
    11701 ttatgttaaa accatctatc aatgaggtac tagaaaaaat agacaacaga tactatttag
    11761 tggggactgt ttcaaagaga gcaagaaaat taattgatgg agaagaacca tatgtttcaa
    11821 ataaaactaa ggaaaagcct gtatgtgtag caactaaaga agttgcaagt ggaaaaataa
    11881 catatagatt attgacagaa gaggaaatag agatagaaga agctagacat catgctgagc
    11941 aacaccaaca aatttcagaa gaggagtaag gtcatgctaa aagataaaac tgttgtaatt
    12001 ggcataagtg gcggtatagc tgcatataag gcttgtgatg ttgtaagtaa gttaaaaaaa
    12061 ctaaatgcaa atatccatgt tataatgaca aaatcagcaa cagaatttgt taaaccactt
    12121 acatttcaat cgctgagtca aaattatgta gtagaagata tgtttgaaga accaaaaact
    12181 tgggaagttg aacatatatc attagcaaaa aaagcagatt tattcttaat agtgccagca
    12241 agtgcaaatg ttataggcaa gattgcaaat ggtatagctg acgatatgtt gacaactact
    12301 gttatggcaa caaaagcaca ggttcttata gcaccagcta tgaatactaa tatgtatgaa
    12361 aatccaattg ttcaaaaaaa tatacaaact ttaaagagct ttggatataa atttattgaa
    12421 ccggaaagtg gaaggcttgc atgtggagat attggaacag gtaaactagc taatccagat
    12481 ataatagtag aggaagtttt gactaatctt actaaagagc aagatttaaa aggtcaaagt
    12541 atgatagtta cagctggtcc aactgtagag agtatagacc cagtaagata tttaactaat
    12601 aggtcttctg gtaaaatggg atattctata gcaagagaag ctatagctag aggtgctgat
    12661 gtaacattaa tatcaggtcc tacaaatctt tctattccac aaaatcttaa aaaatttgtt
    12721 caaatagagt cagcacaaga tttgtatgaa gcagtagtat caaatttaag tgaaaataaa
    12781 atagtaattc aaagtgcagc agttgcagat tataaaccaa aaacttattc tgacaaaaaa
    12841 attaaaaaga agaatgatga tttatcaata gaactaagta gaaattatga tattgcttat
    12901 gaaataggaa aaataaaaga agataagata ctaattggat ttgcagctga aactaatgac
    12961 cttatagaac atgccaaagg aaaaataaaa aagaaaaatt tagattttat tgtggcaaat
    13021 gatttaacta aagaaggtgc tggttttaga gctgatacta atattgtaaa aataatagat
    13081 gtagagggaa atattactga gtatcctaag atgaaaaaag aagaagtagc caatgttatt
    13141 ttagataaag ttaaagcgtt gcttgagaaa taagaccttt tttaaatatg atatttgtgt
    13201 attttatcac agctaaagtg gctgaaagct gcaatttaat tttagcgagt aaaaactcgc
    13261 aaatgtcagt taaattgtag ttttcagcat ttattttgtc ataaagtata aaatataatt
    13321 aatagttaat tattaaaata aagttaatat taataacttg aaattgctta taaattctga
    13381 gattttaata tcttgtaaag tatattaaaa tatgaacaat atattaaatt taagtagagg
    13441 ttttataatg aaaaaatatg caaaggtaat agttagaagt aatactatat atacagataa
    13501 tttatttaca tatcaaattc ctgtattttt atctgatgta ataaaaattg ggcacagaat
    13561 attagtacca tttggaaaag gtaataaacc aacagaagcc tttgtatttc aatttacaga
    13621 tagcttagat gaaaaaataa aaataaaaga aattattgat atattagatg aaaatccgat
    13681 atttagaaaa gaagatttag agttggttta ttggatgaaa aatagatatt tgtgtactta
    13741 tatagaatgt ataaatttga tatatccaaa gggatacaaa ttaaataatt ataaagtagt
    13801 cctattaggt gaaagtttaa gtggattaaa tgatattgaa ttgaaggaaa aaatatcaac
    13861 tttaagtgat aaaaatagag aaatagtaga gaaagtgatt gatagtaaag gtaaaattaa
    13921 ggttgataag ttaaaatata taccaaactt aaatagttct ttgtacacaa tgaataaaaa
    13981 tggaattata aaattatgtt gggaatataa aaatcacaaa aatgaaaaga aagtatgtta
    14041 tatatctttg agtttagaaa gtgataagat tgacgattat atagagcaaa ataaaataaa
    14101 tgtgggaagt aaacaaaaag agatattaag ttttcttaaa aataatgaaa atgtagagat
    14161 aaatgattta ttggatttat taaatgcttc aaaacaaagt ataaattctc taagtaagaa
    14221 aaagctaata accctagaat ttaaggatta ttatagagag ccaaaaagta tttacaaatc
    14281 cgtactaaaa agtataaaat taaacaatga gcaacaagaa gcagttgatg agataaagtc
    14341 gaatatgttt gtagatgata aaaaaccata cttaattcat ggagtaactg gaagtggaaa
    14401 aactgaagtt tacatggaaa taatagaatt tgctctaaat caaggcttag acagtatatt
    14461 cttagtacct gagatagcat taactcctca aacaatagat aggctaaaga gtaggtttgg
    14521 agatttagtt ggagtgttcc acagtaaact atctgaagga gaaaaacatg atgtttacaa
    14581 agctgtcaaa gcaggtaaag taagagtgtt aattggagct aggtcagctt tatttgcacc
    14641 ttttaattcc ttaggcttaa taataattga tgaatgtcat gagtcatcat ataagtctga
    14701 gaagaatcca aaatttaatg ctatagaagt tgctagattt atggcattaa aaaataatat
    14761 aactcttatt ttaggttcag caacgccctc aattgaagag tattataggg caaaatctgg
    14821 agaatacaaa cttataaata taaaaagtag agcaaatgat aagcctcttc caaacattga
    14881 agtggtagac atgaaagatg agttagataa aggaaataga agtatattta gtttgaaatt
    14941 acaaaaagaa atacgttatg cgattgaaga aaataatcaa gtaatactat ttttaaacag
    15001 aagaggttat gcaaattttg tatcttgtag aaagtgtgga tatgtgtttc aatgtgaaaa
    15061 ttgtgatata tctttaactt atcataagaa aagtaatact ggaagatgcc actattgtgg
    15121 atatgagaaa gaaattccaa aagagtgtcc tgaatgtaaa agtacttatg ttaagccatt
    15181 tggagttgga actcaaaaaa ttgaagagga attaaaatat atttttccag atataaaaac
    15241 tttgagaatg gataaagata caacttcaaa aaaaggtgct ttagatgaga tactaaataa
    15301 atttaaagat aaagaagcag atgtacttat tggaacacag atgttaagta aagggctgga
    15361 ttttgagaat gtaactttag taggtatttt gtctgcagac atgatattaa attttccaga
    15421 ctttaaaagt tttgaaacta catttcaatt gataactcaa gtctctggaa gagcagggag
    15481 agctgataaa gagggaaaag tagtacttca gacttatgat actgaacact atgctattaa
    15541 gcatgctatt gaatatgatt atgagggttt ttatgaagat gagataaaaa taagaaaagc
    15601 atttggatat tctcctttta ataatatgtt aagtgtagtt gttagtggtg aagatgaaag
    15661 attagtgata aaaaatataa aaaatatgca tgcatcattg atttatttat tagaaaaaag
    15721 gggtataaat gatttaggat ttatattagg tccaaatcca tgttcaatat caaaaataaa
    15781 tcaaaattat agatggcaaa tactctttaa agatgaaaat attgaaatta atctcttaaa
    15841 gggtataata aaatatatat gtataacaaa acgagatttg atatttgata agaatatcaa
    15901 tgtatctata gatattaatc caaacagtgt attataaatt taggaggaat tataatggct
    15961 ttaagacaaa tagttcaaat aggagaacca gtattaagaa aaaaatcaaa aaaggttgaa
    16021 aaaattgatg aaaagataat acaattatta gatgatatgg cagaaactat gtatgatgca
    16081 gatggtgttg gactggctgc acctcaagtt ggaatattaa aaagagtagt agtaatagat
    16141 attggagaag agcttataga acttataaat cctgaaataa tagaaacatc tggggagcaa
    16201 atagatgagg aaggatgttt aagtgttgtt ggagaagcgg gaaatgttag aagaccaaat
    16261 tatgtaaagg tcagagcttt aaatagaaat ggtgaaacaa tagagttaga gggagaagaa
    16321 cttttagcta gagcattttg tcatgagata gaccacttag atggaatttt atttgttgac
    16381 aaaatagaaa agtagtagga gttgatttga tgaaaatagt atttatggga actcctgata
    16441 tagcagttcc atgtttacaa aaaataatag atgagaagta tgaaatattg ggtgttgtaa
    16501 ctcaaccaga taaaccaaaa ggaagaggta aaaaactagg tatgagtcca gtaaaagaac
    16561 tggcaattga aaataatata cctgtatatc aaccagtaaa ggctagagat aaagaattta
    16621 tagataaaat taaatcttta aatccagatg taatagtagt tgtagctttt ggacagatac
    16681 ttccaaaaga aatattagag attcctaaac ttggatgtat aaatgttcat gtttctttac
    16741 ttccaaaata tagaggtgcg gcacctataa attgggtaat aataaatggt gaagaaaaga
    16801 ctggtgttac aactatgtat atggatgaag gattagatac tggagatatg atactaaaga
    16861 cagaagtaaa tcttgatgaa aatataacag caggagaact tcatgataaa atgatgaata
    16921 taggagcaga aactttaaaa gaaacattaa gattaattga agaaggaaat gttccaagag
    16981 aagtacaaaa tcatgaagaa ttttcctatg cgccaataat gaataaatca ttaggtaaca
    17041 tagatttttc aaaaagtgcc agagaaatac acaatttagt tagaggagta aatccatggc
    17101 caagtgctta tactacctat aatgatgtca taatgaaagt ttggaaaact aaagtgttag
    17161 atgaaaaaag cacaaaagat gtaggtacaa taattgatgt aagcaaagat gggataaaag
    17221 taagtactat agataatgta cttttaattg aagaaattca gatgccgaat aaaaagagaa
    17281 tgttggttgg tgaatatata aaagggaata ctatagaaac tggtttagta ttgggttagg
    17341 tgaaaagtat gacagatata aagaagtatt taacaagtgt tgggattttg attttaatat
    17401 taggaatttt gataggagtt gttaattttt ttataataag tttaactcaa atattctcct
    17461 tgatgataaa tattataata atatcactaa tacttataat actatgttca acagcagtta
    17521 cttatagagt aattaaagga aagtatgtta actcaaatat aatgaaaata aattttaata
    17581 ttgtcagtgg attatttcct attatatctt tcattgcatc atcatttggt atgtcaaaaa
    17641 gtgatattag aagaatatat ataaagttaa ataatgaata tatatacagt aataaatata
    17701 attttaatcc agaggatata ataattttaa tacctcattg tattcaagag aatagttgta
    17761 aattaaaagt tactaatgat atagataatt gtaaagaatg tggaagatgc aacataggag
    17821 aattaattaa gctaaaggaa aaaactaatg taaaaatatt tgtagctaca ggaggaaccc
    17881 tagctagaaa aattataatg gacactaaac ctaaagctgt tgtagctgtt gcatgtgaaa
    17941 gagatttgac atctggaata caagatatta aaaaaattcc tgttttaggt gtatttaaca
    18001 aaagacctaa tggaccttgt gtaaatacta atgtagacat gatggatatt gaaaaagcta
    18061 taggcttttt aacaggaaaa aatatattag tttgtaatta aactgttttt aagttaagtg
    18121 aatattaaga ggatgtataa taaaaatata ttttaggaga gtgttattta tgtaccctgt
    18181 atatggcggt ttttggggat ttgacccaac aatggttgta ttaattccag ctatattact
    18241 tactatatat gcacaattta aagtgagttc aacaacaaat aagtatttaa gagtaaatac
    18301 acgtcgagga tatactggag agcaaacagc aagaagagta cttgattcaa atggtttgta
    18361 tgatgtaaag atagaaatgg ttagagggca tctaagtgac cattatgacc caagaagaaa
    18421 ggctgtaagg ttgtcagaag atgtatatta tggaacatct ataacttctg ttgcagtagc
    18481 tgcacatgag tgtggtcatg ccatacaaca tgctaaaggt tatgcacctc ttcaaataag
    18541 aagtagttta gtaccagtag tgaattttgc ttcaagtata tcttggtttt taatattttt
    18601 aggatttatt atggctggtc catttttaaa aattggtata ctattgtttt cggcttcagt
    18661 gttattccaa ataataacat tgcctgtaga atttaatgct tcaagtagag caatcgttca
    18721 acttgggaat ttagggatta tagatgaaag tgagtcaaga caaagtagaa gagtactttc
    18781 agcagcagct ttaacatatg ttgcagcagc attggtttca atacttcaat tacttagatt
    18841 gttactaata gctcaaagaa gaaatgacta aaagtaaagt gggttgttta gaaacaaccc
    18901 ttttttatgt ttaataaaag ggagaaattt atatggatgc aagagaagtt ggatttaaag
    18961 tattatgtga tatagaaaaa aatgacaatt attcaaatat tgcaataaat aagcatttta
    19021 aaaatttaga aataagtgat atggatagag gacttgcaac agagttaatt tatggagtaa
    19081 tagaaaataa atattattta gattatataa tcaataaact ttcaaaaata aaggtaaaaa
    19141 aaatgtcaac ttatgttaaa atctttttaa gaatgggaac atatcagatt ttatttttag
    19201 atagtatatc agattatgca gcagttaatg aaactgtaaa attatctaag aaatatgata
    19261 aaaaatcttc tggtttcata aatgctattc ttagaaatga aataagagct aaagagacta
    19321 taatggacat aactgaggaa gatagtgtaa aatacttatc tataaagtat tcctataact
    19381 cttggattat aaaaaactgg atagataaat ttggacaaga atttgctgaa gatttgttgg
    19441 aagcaaataa tgaaaagcct agtatctata taagaacgaa tacacttaaa ataagtagag
    19501 aagaacttat tgaaaaatta agtgaaatgg gtattgtatg tttaaaagtt cctatggtag
    19561 aagaagctat taaagtcgaa aaaatgaaaa atatagaaaa aaatgaatta tttaaggctg
    19621 gtttatttac aattcaagat ataagttcta tgatagtagg gaaagtaatt aatccaaaag
    19681 aaggttcatt tgttttggat gtatgtagtg ctcctggagg gaaatctact cacttagcta
    19741 ctttaatgaa caatactggt caagtagtag ctagagatat ttttgagcat aaactaaagc
    19801 ttataaactc tactgtaaat agattggggc ttaaaaatgt gtttgtagaa agatttgatg
    19861 cttctgagat tgatgaaaat agtataagta aatttgatta tgttttagca gatgttcctt
    19921 gctctggttt tggtataata agacgtaaac cagaaattaa gtataagaaa gaagaagagt
    19981 taaaagacat cacttctata caaaagaaaa ttttagaaaa tgcatctaaa tatgtcaaag
    20041 ttggtggaac tttagtatat agtacatgta caattcaaga tgtggagaat ataaatatag
    20101 ttacttcttt tgttgaagaa aatcacaact ttgaatttgt tccaattgat acagtaaatg
    20161 tagacttaga taatcaagat aaaggatatt taaagatata tcctaatatt catggtatag
    20221 atggtttttt catagcaaaa ttaaagagaa taaggtagtg agagaatgga aaagaaaaag
    20281 atagtattaa aaaattttac tgaagatgaa cttaaagaat ttatgaaaac tatagatgaa
    20341 aagcccttta gaggaagtca aatattctct tggatatata agggtgcaaa aacttttgat
    20401 gatatgaaca atataccaaa aagtttgaga aataaattag aagaggtttc atgtataggg
    20461 catatagata tagaattaaa gttagaatct aaggtagata atacaaaaaa atatttgttt
    20521 ttattagatg atggaaatat aatagaaact gtgatgatgg attatgatag tagagttact
    20581 gtctgtgttt ctaaccaagt aggttgtagg atgggatgta atttttgtgc atctacaatg
    20641 gatggattaa ttagaaactt agagccatgg gaaattttag accaagttat taaaattcaa
    20701 gaagatactg gtaagagggt atcaaatctt gtattgatgg ggagtggtga gccacttgat
    20761 aactttgaaa atacaaaaca atttttaaaa ataattaacg aaaaaaatgg tcttaatata
    20821 gggtatagac atataactct ttcaacttgt ggaatagtac ctaaaatgta tgagttggca
    20881 gacttagaaa tagctataaa tttagcttta tcacttcatt caccatatga tgaagaacga
    20941 agaaaaatta tgcctgtagc aaatgcttat tctattgaag aaatattgaa tgcatgtaga
    21001 tattatataa aaaagacaaa tagaagggtt acatttgaat attctcttat aaaaggggta
    21061 aatgactcag aaaaagaagc aaaggcatta gctaagctcc taaaaggtat gctatgtcat
    21121 gtaaatttaa ttccaataaa taaagttgaa gaaagagaat acgaaaaacc agataaagca
    21181 tttatttata aatttaggga tagtttggag aagaacaata tacctgccac agttaggatg
    21241 tctatgggtt ctgatattag tggagcttgt gggcaattga gaagaaaata caagtaattt
    21301 gtaaggagga agtcgtatgg tttatagttg tgcctcccat ataggaaaaa taagaaaaaa
    21361 caatgaagac tattgtgagg gagaagttat agatacagaa catggtccaa taggaatatt
    21421 tgccatagct gatggaatgg gtggacataa aaaaggtgaa gtcgccagta aattagcagt
    21481 agaaaatata attgattttc taaaggaaaa cttattacaa catgataatg taaaaataga
    21541 ctatatagat gatatattaa aacaggctta taataatgta aactcaattg tgcacaaaaa
    21601 atctatggaa gatatagaat ttgaaggaat gggaactaca ttagttacgg ctatagtgta
    21661 taataatgtc ttatatgtgg caaatgttgg agatagcaga tgttatttgt taacagatga
    21721 aaaatttgat aaaataacta tagaccattc cgtggttgaa gaacttatga tggcccatgt
    21781 cattacagaa gaagaggcaa gaagacatcc acagaggaat agaataacta gagcaattgg
    21841 aacagatgat atggttgttg tagatatttt taagaaagaa atcaaaaaaa gtgatataat
    21901 acttcttgcg acagatggat taactgggtt tattgatgat gaagatataa gagatttaat
    21961 attagattat gaatatgaga gaactagtaa tataagtgaa gaattgataa gtatggccaa
    22021 tgatgtttct ggtaaagata atgtttcggt tatagtgata aaagtataat taggacggtg
    22081 atattgtggg agatacaatt ttaggaaatc gatatgaaat catcaggaag attggtgatg
    22141 gaggaatggc ctttgtatat gaggctaaag acagattatt aaacaggact gttgcactta
    22201 aagtacttag acctgaattt gtagatgatg atgaatttct aacaaagttt aaaagagagg
    22261 cagaagcagt agcaagtctt tcacatccaa atatagtaaa tgtttatgat gttggtgaag
    22321 atggaaaagt tcactacatt gtaatggagt ttgtagatgg taagaatcta aaagagatta
    22381 tacaagatga aggtatatta gatgaatata ctgcattaga tataacaaaa caaatagcta
    22441 tggcacttag tgcagcgcac aaaaagggga ttatacatag agatataaaa cctcataata
    22501 ttctcatatc aaatgaagga agagtagtaa aagtggctga ttttggtata gccaaagctg
    22561 tttcaaattc aacaatgact aatattggta gtataatagg ttcagtgcat tatttttcac
    22621 cagagcaagc taaaggtaaa tttgtaacta ataatgctga cctgtattct ctaggtatag
    22681 ttttatatga aatgttgata ggaaaagttc catttagggg agatagtcca atttctatag
    22741 cacttcaaca cattaatgat gatatagact ttacatcaga agaaaaagtt aggattcctc
    22801 aaagtgtaag aactacaata aaaaagctta cagaaaaatc tagtgcagat agatatcaaa
    22861 ctgctgaaga attgatagaa gatatagagt atatagaaaa aaatattgat ttagatttta
    22921 taaaagaata tgatgatttt gcaactaaga aaattgatga aaaagaaatt aataaagttg
    22981 taaatccaac acttgcaaaa ccagctcctg aaaaagtggt taagcctgtt gaagtagctg
    23041 atttggatga tgatgaagat tactatgatg atttttatga agaagatgat gatgaagatg
    23101 aagaagagga agagataatg agagctaaaa agaatcagag acctaagagt actccaagta
    23161 agagaactaa gaaaaagaag aaaaaacagg aaagcccaaa atctagaaga agattaaaag
    23221 taattgcggc tgttttgata ctgatattgt gtgctcaagt attcttagca tataagtttt
    23281 tatttgctgg agggtttggc aataaatctt tgactgttcc taatcttgta aatatgacat
    23341 tagaagaagc tcaaagtgct gttgaaaaag aaggattata cctgagtgta aaaagtgaag
    23401 agtataatag tgaagttgat gaaaattgta taatatcaca gacacctgaa ggtggaagta
    23461 caaatgtcaa aaagggtgat actataaatg ttgtcgtaag taaaggttca agtcaggcat
    23521 ctgttcctaa tgtagtggga cttactttga gtaatgcaaa acaacttatt gaagaaaata
    23581 acttaaaagt gggaactgtt aagtatgaat acagctcaat atataaagaa ggtacagttt
    23641 taagtcagag tccgggtgct ggctcatcaa gagctcagga aggtgatgag gtaaacctat
    23701 atgtaagcaa aggttcagaa aaatcaaata cacagactcc aacagtacca gataaaaaac
    23761 caacaacacc agagaataat acaccaacag aacctggttc aaactcagga aacagtggtg
    23821 gaaattcagg aggctcaaat tcaggaaata gtggtggaaa ctcaggcaat aatggaagta
    23881 actcaggagg ttcaaactca ggaaatagtg gtggaaactc aggaggctca aactcaggag
    23941 ataatggagg aagttcagga ggctcaaact caggagacaa tggaggaagt tcaggaggtt
    24001 caaactcagg agacaatgga gatagttcag gaggctcaaa ctcaggagat aatggaggaa
    24061 cttcatcagg aactggcgca aacattggta aaacagaata aatattaata aaataagagc
    24121 tgtcctttta tagaaaatat tttctttaaa gggcagtttg ttttgtataa aatactgaat
    24181 tggttataat ataaggagaa aagtatttat gaagaagaaa acagttttaa ttacaggagg
    24241 agctagaggg attggtaaag ctatgtctaa agcttttgca aaagaaggtt acaatgtatt
    24301 agtaaatttt aataagtcag aaaatgaagc taaagagttg tacactatat taaatgaaaa
    24361 aaatttttct gtaaaacttt ttaaggctga tatttcaaat agagaagatg tagaggatat
    24421 ggtagattat tgtataaaag aatttggagg attggatgta cttgtaaaca atgctggtgt
    24481 aagtcaagat aagttgttta cagatataac tgatgaagat tgggataata tgatgaatat
    24541 taatttaaaa ggaagctttt attgttcaca agttgcttta aaatacatga tatcagaaaa
    24601 aaaaggaaat ataattaata tatcttcaat ctggggtata tcaggtgcgt cttgtgaagt
    24661 gcattattct ataacaaaag caggcataat aggtatgaca aaggcattag ctaaagaagt
    24721 aggaccatca aatattagag taaatagcat agctccaggt gtcattaata ctgacatgtt
    24781 atctggatat aatgaggaag atatagacgc tcttgtggaa gaaacccctc taatgaggct
    24841 aggaactcca gaagatatag caaattgtgc tattttcttg gcatcagaca aatcaaattt
    24901 tatcactggt caggtaatta gtccaaacgg aggatttgtt atttaaaaaa aatgtggtat
    24961 tatataaaaa taggaaagaa taacacgata tactactcat aaacagtatg tgtttatata
    25021 aattttataa agggaggcat ttgatgctag aaggaaaaat tataaagggg attagtggat
    25081 tttattatgt agatacttat aatggtattt atgaatgtaa ggctagaggg atatttagaa
    25141 agcagaaaat aacacctttg gttggtgata gagtcaaaat aagcattgtt gatgaaaatg
    25201 aaaaaaaggg tatcttagaa gaaatcgaca gtagagatac agaacttata agacctccta
    25261 ttgcaaatgt agataaagca ttaatagtat tcgcaataaa aaatcctaaa ccaaacttat
    25321 ctcttttaga tagatttata gttttggcag aaaaagaaaa tttagaaact gtaataatcc
    25381 ttacaaaagc agatttagat gataatgata cattagaaac tgttaaaaat atatatgaac
    25441 ttagtggcta taaagtcatt cctgtaagca atattactaa gttaaatata gataaagtaa
    25501 aagaagaatt aaaagaaaat gtagtagtgt ttgcaggacc atctggagtt ggaaaatcaa
    25561 gcttattaaa tgaaatagat gaaaatttta aacttcaaac aggagttgta agtgataaaa
    25621 taaaaagggg aaaacatact actcgtcacg ctgaactttt aaaattagaa tttggtggaa
    25681 tggttgcaga tacaccaggt tttagttccc ttgcccttga agatatagag gaagttgaac
    25741 ttaaagatta ttttatagaa tttgataaat ttaatgattg taagtttggt tcaaagtgta
    25801 ttcatgaaaa tgaacctaat tgtgctataa aagaagctgt tacaaatgga gaaatatcta
    25861 aagaaagata tgatagttat atacagctgc tacatgaaat aagacaaaat aatagtagga
    25921 ggtattagaa acatgattaa attagcacca tcaatattat cagcagattt tgcaaaatta
    25981 ttagaagatg taagaaaagt agaaagtgca ggatgtgaat atcttcatat cgatgtaatg
    26041 gatggacact ttgtaccaaa cattacatta ggaccacttg tagtcaaaag tttaaagaaa
    26101 gaaaatataa atatggtatt tgatgctcat cttatgatag aaaatccaga ccagtatatt
    26161 gaagaatttg taaaagcagg atgtgatata ataactgttc accaggaggc ttgtgttcat
    26221 ctacatagaa ctatacaaaa tataaaatca catggaataa aagctggtgt agtacttaat
    26281 ccagctacac cagtagatac gattaaacat gtactacctg atttagatat ggtattatta
    26341 atgtctgtta accctggatt tggaggacaa tcatttattc cttgtgtttt agataagatt
    26401 aaagagttaa aagctattat agatagtcaa ggattaaaca ttgatataga agttgatggt
    26461 ggtataagcc ctaaaaacgt agccgaagtt gtacaagcag gagctaatgt aatagtagca
    26521 ggttctgcaa tatttggaag tgatgacatt caagaaacag ttaatttatt tagaaagaat
    26581 gcttctcttg aagaattagt ttaaatttaa taatgaaaat ctgtattgtt ttaaatggcg
    26641 aaattgaaga ttacgaagtt acaagagata ttataattaa ggaatgttat gactgcataa
    26701 tatgtgcaga tggaggggca aatcacactt ataaaatgga aataatgcca gattatatat
    26761 taggagattt agattcagta gaagaagaaa agataaactt ctataaaaat aaaggtgtta
    26821 aatttgaaaa atttccatct aaaaaagatg agactgatac agagctttgt ctttttttag
    26881 ctaagacact taaagcaaat catattgatt tttttggtgc tttaggggga aggattgacc
    26941 acactcttgc aaatataaag ctattatatt atttaaaaga agatggtata tattctagga
    27001 tactttctga taaagaagag atgtatatag ttgaaaatga ggaaatttca ttgtatggaa
    27061 acccagggga cactatttct gtaatagcta taaatggtga tgctaagggt gtaacactta
    27121 ctggcttaga ataccctctc gataattatt atatgaaata ctctgtacct attggaatat
    27181 caaatgttat gttgagtaat tcttgtaaga ttaaagtaga gcaaggctgt gtacttgtag
    27241 ttagaaattt ataaaatttg attttgacaa ttggttttat attgtagagc agatacaatc
    27301 aagtaaaata tatttatgat tgtatctgct ttttagtata taattataaa tatataataa
    27361 actttgacat aagggggatt ttttgtggta gatagtatta tttttgattt agatggaaca
    27421 ttgtgggatt caactgaaga agtatgcaaa gtctggcagg atgttttaag tgagcataag
    27481 gaaatagaat taagtgtcac aaaagattta tttagaagtc ttatggggct ttcttttgag
    27541 gaaatagcaa atcgtttatt tccaaattta agtgaagaag aaagaatgaa tttattgaat
    27601 gaatgttctt tgaaagaatg tgactattta tctgaaaatg gagctaaatt atttgatgat
    27661 atagaagata ctcttaaatt actatctaaa aaatataaat tatttatagt aagtaattgt
    27721 caagctggat atatagaatc ttttttaaaa gcacataaac tagaacagta ttttatagat
    27781 tttgaatgtc ctggtaatac tggtttgcat aaaggagaaa ataataaact tataattgaa
    27841 agaaataaat taataaatcc aatctatgtt ggtgatacgc aaggtgatgc aaatagtgct
    27901 aagtttgtag gtataccttt tatatatgcc aaatatgggt ttgggaatgt agatgtatat
    27961 gattatttta tagattcatt taaagatttg ttagaacatg atatattaaa ataagatgag
    28021 tttaaaaatt atattaaaat aataaaaaag ttatgggggg atttaaaaat ggaattacaa
    28081 gatactattt ttaaaagaca aagtgtaaga aagtttaaaa atcaagatgt atcagatgaa
    28141 gatattttaa aaatgataaa agcagcaggt gcagcaccat caggcaagaa tattcaaaac
    28201 tggcattttg ttgttataaa acgccgtgat ttaatggaaa agatagcaga tgtgataact
    28261 aaaaagcaac aagaaatact tgtagaaatg gataaagtat cagtggataa ggctaataga
    28321 tttagaaaat ttgttaaaaa ttttacacta ttttacttaa aagctccagt tttagtatta
    28381 gtttttacaa aggtatataa tccatcagga tattatgaat tagagcttat agatgcacct
    28441 aaggagacta tagataaatt atttataaga aatccaggta tgcagagtct aggtgctgca
    28501 attgaaaact ttacactatc agcaattgaa cttggatatg gttcttgttg gttgacaagt
    28561 caaaactatg cagcagatga gatagaagct gttttagaag cagaaactgg ttttgaaaag
    28621 ggagagtatt ttttaggtgc aatgttagct cttggagtgc cagaggacaa tttgaagagt
    28681 ccatctaaaa aaccagtaga agaaatatgt acatttataa aataaaagtc aaggacattt
    28741 taaaatcgaa gtaaattaaa aacaaaataa attaaaaata aatatttaaa aaattaaaaa
    28801 gtaggtttta ttgatattaa ttttatattt caaatcattg atttaattaa tacaataaga
    28861 actacttttt ttgtatatta attttaaata tatttaaat
//
LOCUS       DAELJO010000028        28304 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELJO010000028 DAELJO010000000
VERSION     DAELJO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.028"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(34..894)
                     /locus_tag="KPB88_001110"
     CDS             complement(34..894)
                     /locus_tag="KPB88_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG0053408.1"
                     /translation="MAMKWRDYAADVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1211..2539)
                     /locus_tag="KPB88_001111"
     CDS             complement(1211..2539)
                     /locus_tag="KPB88_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0053409.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2687..3016)
                     /locus_tag="KPB88_001112"
     CDS             complement(2687..3016)
                     /locus_tag="KPB88_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0053410.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3040..4329)
                     /locus_tag="KPB88_001113"
     CDS             complement(3040..4329)
                     /locus_tag="KPB88_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0053411.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4345..4671)
                     /locus_tag="KPB88_001114"
     CDS             complement(4345..4671)
                     /locus_tag="KPB88_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0053412.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4806..5435)
                     /locus_tag="KPB88_001115"
     CDS             complement(4806..5435)
                     /locus_tag="KPB88_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053413.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5650..8307)
                     /locus_tag="KPB88_001116"
     CDS             complement(5650..8307)
                     /locus_tag="KPB88_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053414.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8666..9610)
                     /locus_tag="KPB88_001117"
     CDS             complement(8666..9610)
                     /locus_tag="KPB88_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053415.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDISKYQQNLKVV"
     gene            complement(9603..10628)
                     /locus_tag="KPB88_001118"
     CDS             complement(9603..10628)
                     /locus_tag="KPB88_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053416.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10703..12271)
                     /locus_tag="KPB88_001119"
     CDS             complement(10703..12271)
                     /locus_tag="KPB88_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0053417.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12384..13295)
                     /locus_tag="KPB88_001120"
     CDS             complement(12384..13295)
                     /locus_tag="KPB88_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053418.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13279..14265)
                     /locus_tag="KPB88_001121"
     CDS             complement(13279..14265)
                     /locus_tag="KPB88_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053419.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14625..15026
                     /locus_tag="KPB88_001122"
     CDS             14625..15026
                     /locus_tag="KPB88_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0053420.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15029..15427
                     /locus_tag="KPB88_001123"
     CDS             15029..15427
                     /locus_tag="KPB88_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG0053421.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15876..16100)
                     /locus_tag="KPB88_001124"
     CDS             complement(15876..16100)
                     /locus_tag="KPB88_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG0053422.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16135..17502)
                     /locus_tag="KPB88_001125"
     CDS             complement(16135..17502)
                     /locus_tag="KPB88_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053423.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17586..19121)
                     /locus_tag="KPB88_001126"
     CDS             complement(17586..19121)
                     /locus_tag="KPB88_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053424.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19144..19578)
                     /locus_tag="KPB88_001127"
     CDS             complement(19144..19578)
                     /locus_tag="KPB88_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053425.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEVIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19838..20749)
                     /locus_tag="KPB88_001128"
     CDS             complement(19838..20749)
                     /locus_tag="KPB88_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG0053426.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            21045..21398
                     /locus_tag="KPB88_001129"
     CDS             21045..21398
                     /locus_tag="KPB88_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0053427.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            21478..21650
                     /locus_tag="KPB88_001130"
                     /pseudo
     CDS             21478..21650
                     /locus_tag="KPB88_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            21819..22283
                     /locus_tag="KPB88_001131"
     CDS             21819..22283
                     /locus_tag="KPB88_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053428.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            22309..22668
                     /locus_tag="KPB88_001132"
     CDS             22309..22668
                     /locus_tag="KPB88_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053429.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKETNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(22719..23660)
                     /locus_tag="KPB88_001133"
     CDS             complement(22719..23660)
                     /locus_tag="KPB88_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG0053430.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGIDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(23761..24996)
                     /locus_tag="KPB88_001134"
     CDS             complement(23761..24996)
                     /locus_tag="KPB88_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG0053431.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(25040..25894)
                     /locus_tag="KPB88_001135"
     CDS             complement(25040..25894)
                     /locus_tag="KPB88_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053432.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(26022..27197)
                     /locus_tag="KPB88_001136"
     CDS             complement(26022..27197)
                     /locus_tag="KPB88_001136"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG0053433.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(27296..28180)
                     /locus_tag="KPB88_001137"
     CDS             complement(27296..28180)
                     /locus_tag="KPB88_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0053434.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         9113 a         4988 c         3014 g        11189 t
ORIGIN      
        1 taataagtat ttttaggatt ctgttacata atattattta aatattcttc tatatttctc
       61 ttcttcttct tcagtaaaca cattaaatac aacttttaaa tttttttcag gattttcttt
      121 tagccatagt ctaactctac ttacagcaag attagctgcc tctttttttg gataaccaaa
      181 tacacctgta gaaattccac aaaaaacaat attttttatt tcatcaattt cttttattgt
      241 atttaaacat gatttataac aatgaagcag ttgcttttct tgctctttac ttggctgacc
      301 accagataca ataggtccaa cagtatgtac tacaaatttt gcaggtaaac aataacctct
      361 agttatctta gcatctccag tatattctaa atgcccttgt ttttttatta ttttgtcaca
      421 atcttctcta agtctaggac ctgcgcatga atgtatctca ttatccacgc atagatgtaa
      481 tggttgtaaa caccctaata acttgttatt tgcagcattt actattgcat ctgctcttaa
      541 attcgttata ttacctctcc atatggcaat cccttcctct acttcttcta tatcatttac
      601 atctactaac tctctttctt cacattcaat caacaataaa ttatgtaaca tattaagtat
      661 ctctggagca atttcttttg gtggatatac attcaaaagt cctctaagta tatctctagg
      721 cttaatttca tcatttgata aattgtatat tttagaagga acttcttttg aaaaatatgc
      781 aattaaagtg tttatatttt ttcttctttg ttcgtcagtt agaggcttga tagttttatc
      841 aaaatcctca aataaattta catcagcagc ataatctctc cacttcatag ccatctctcc
      901 attcttgtat tttagactta aaaataatta tgtatttatg tacatattta tttatttcta
      961 taaacaccta cataaatcct gttaaaagtg taaatttttt attatgcaac ttctcttgtt
     1021 atttctattg attgatagta aattattgta cctaaattta atccaactaa caagtaacta
     1081 aacctacttt cctaactgca aaatacacta tgtttgccac ttgtatctca tttgtagata
     1141 aataaagtcc ctacaaatac attaactagg ctatgtacta aactgtagga actttaaaaa
     1201 tatattattc ttaattaagt tttggccaat aatttttatt tgcttctatt aaatcatcta
     1261 gtaattcttt tgcaactgtt gcactaggaa ctgtcttaga tagcataagt gcttgccaca
     1321 atttattata agacttctca atccaagcat caactactaa tttttcaact gctaattgtt
     1381 gttctattaa acctttttgg aatgttggta ttttccctat tgataatggt tcatacccgt
     1441 cttttccaac tatacatgga atttcaacca tagcatcatc ttgtatattt tctatagctc
     1501 cattattttg aactatcaat aacatacgct cctgtgtatt aaaagctaga gctgtagcta
     1561 aatccactat aaactctgca tgtattccta tcttaagccc actgccctta gaatgtcctt
     1621 gtttttctat caatgcacat gaaccaaaaa cttctttttc tcttccatca ataacttcat
     1681 tggcacgagt ataatttaca tcagaatgat ttacaacata atctggaaat agatagtact
     1741 ttaagtaact acttggtatt gtagttaaat ctgtttttac tatttcttta gtaaataaat
     1801 tagtttcaaa ccaactatct ttcttgtcat ttttcattcc ttcttcacca tttataaaac
     1861 catattcttt tatatgttct actaattttg gtaaaagttc atttcctttt ttatctttaa
     1921 ttgaagtata ccaaccaaag tgatttaatc caaagtatct tatatccata tgtttacgag
     1981 atttgagtcc tgcaatcctt gctatattat gttccatacc tattggcata tcacaaatat
     2041 ttattaccct agaattaggt ctcaaccttc taacagcttc cgctattatt gatgctggat
     2101 tagaataatt taacatccaa caatttggag aatacttttc catataatct atgttttcaa
     2161 gaacaccttc tatagacctc attccataag ctattccacc tggtccacaa gtttcttgac
     2221 caactactcc atgttttaga ggtatctttt catctagttc tctcatttca agttttccaa
     2281 ctcttatatg agctaaacaa aaatctacat ctgtataagc ctctttaggg catgttgttg
     2341 ctaagtactc tatattaggg tccttttcct ttattagtat ttcacaagct ttagctacct
     2401 tattttgtct ttcttcatca ttatcatata atttgattgc tcttaatggt aacctatcta
     2461 agttatctag caacattaat atgatttctg gggtatatgt acttcctcct ccagctatta
     2521 ctactgaaaa tttttccata ataatatctc cttttctatt taaaaattta ctgtatttat
     2581 aaataaattt ataaatacta gtactttaaa gtaaaaggga gttcatttat taaatctatt
     2641 gatttaataa actatagcca aacttcaagc actatttaca agaaatttaa ttcttattaa
     2701 ttcttgggtc catcatctca aatatatcta caagttcaga tactaaagaa tgagcaaata
     2761 atgcatttga ataatggtct tcagagtgca caactaaaaa atcagtccca ctatcttcac
     2821 tattcatagc atttgatagt aattctaaat gatgctttga tgctgtttgt attttctctg
     2881 aggctgaatt taaaaggtgt tttgctttat tgtagtcacc tttttttatt tccttaagag
     2941 acatcattac atcactttta gcatctcctg cttctgatat tatactaaat actttttctt
     3001 ccatttcagg taccatatcc ttctccttct aatgacttgt taaaaatcaa agctatcatc
     3061 atctaatgtt gtagctaatt ctgcattttt atttaattct tccttctcca ttatcttaaa
     3121 gaatggataa tatatcaagg tcataagtat tatacttgca aatactaata cgattgcctt
     3181 ccaatctaat gtcatcaaaa atgctgcaac ccctggaggt aaaaagcctg gtggttctgc
     3241 aataggtaat cctactaatc ctacttttaa tgccataaaa ttaagtgctc caaccaatac
     3301 aggtccaaat acaaatggta caaacatata tgggttcata actattggtg ttccaaatat
     3361 taccggctca tttattccaa atagtgatgg taaaatcaca acttttccca tactattaaa
     3421 actcttacac ttacttcttg acataaggta cactaatccc aaagttattc ctgaaccagt
     3481 tacttgcaag aaaccatagt aaaatcctat tgtaaacaca tattttgggt ctcctcctgc
     3541 tgctatcagt gttgcatttt ctgccatata tgtagctgct attggcatcc atatacttga
     3601 agttattgct gctccatgaa gcccaaagaa ccataataat tgagttataa atagtattat
     3661 agttactgca aaaacattat ctacactgct tatagctggt gctaagaaat tcattataat
     3721 agctggtagt ggttgacctg agacattttt agttattgct gctattgctg taaataacat
     3781 tatacatact atcattggag ttaaatttct aaaactttta tttactacac taggtacttc
     3841 ttttggaaat ctaattacga gatttttatc attcataaac ttagtaatat caactactaa
     3901 aagtgctact aatatactta caaataaacc ttgtgcatct aaataacttg tatctaatac
     3961 accatctact acttgtccac atacaacaag ttgtgcaaca actgctgtca tgcctatttc
     4021 tacatgatta agattatatt ttttagctag tgcctgagct ataaaaaatg ctgaatatat
     4081 agataataaa ctcatagtaa atttattagg gataagtaat gtatccatat tttcagataa
     4141 taatgttttt gcacttcctt caggtaacat attgtttaat gccataaata acatacttaa
     4201 agaaccaaca attattattg gggtaatgct tatcatacct tcttttatag cttgaagatg
     4261 cctttgtttt tcaatctttt gtgctattgg aagaagaaat cttcttaaat tattttgaag
     4321 tgcttccatt tttattcccc ccaattatct atttttatat aattcgattg ccgacttcat
     4381 aacagaagga gcatccatac gaccataatg cttagtatct attaaaccaa ctgctatttt
     4441 gtaattctta caagcttcaa ctacatcatc ttccttaaac ctaatttgtg gtgctacaag
     4501 tactacatct gtatcattag cttcaatgta tgattttaaa ttttcaaatg atgttgcctc
     4561 tatatttata gttttagttt ttaacttttc atgctcacta caaaaacttt tcatcttttc
     4621 cacaagtaca cctgttgaag ctccatttgc acatactaat aaaatattca taattaccct
     4681 cctacaatat ctaacctctt ttattttcta attttttatc agccaattct tactctaatt
     4741 cctatcagtt tttactttaa ttcttattat tttattttaa ttcttattaa tatttactct
     4801 aattcttagt actgccaatg attaaacttg aaagccgtat ctataacttc ttcttgctta
     4861 tatttttcta atatttttaa gtatctttct ttataatcat tatctacttc tttttctggt
     4921 attactatac tagcttcatt taaaaagtgg aatgaatccc ttcccatttc taatcctcta
     4981 tatgtatgct tgtcaagtgc cacgtccata acttctggaa ctttacccat tgcaaatcct
     5041 tttataatta tattttttaa taaatcactt gatctatctt tctcacatgc acaaagatat
     5101 cttattgcat gtatgaaaaa taaaggtctg tctccatctg catatgaata ttcctttctt
     5161 acttgattta aattatttat gattattaca gctgatgggt cccccattcc tatatcttca
     5221 actgatattg taagtatcct tctccatagc ttatcttcta attctggtga tgatatatat
     5281 agttcataag caaattcaca agcttcttcc accatatttc ttcttatagc tttttgaaat
     5341 gacgatatta tttcgtctgc actaaatccg tttcttgtag tcattcttgc ccatgggtca
     5401 tttgcctttt tatctcttaa tattttatct tgcatattat ttactccttt ttatttaaat
     5461 tttaattatt tatattactc atttaataat ataagcaagt attatgccag tttttttaga
     5521 cactttaaaa cactgttatt tcaatcattt ttatcatcaa aacttagata ctttgtctaa
     5581 gtatatttat atgtgtctaa tattaatatt attttagact aaaaaaaatt accaaataag
     5641 tttttatact cattggtaat ctttttcgat tatttctgca tattctaata ttaataatat
     5701 ttgatagcac tctttttcat taatttctat attatatttt ttatctaatt gagatactat
     5761 atcttttatt ttgcttagac tcttgtgata atcaagtgtt ttttctttat caagcttttc
     5821 aatttctcta gtatttccaa tcaacccatc taccatgaaa gctaaatgca tcataagtcc
     5881 aattacttta tcgtatttct tttctatatt aaatactaaa aacaattcat cagacacata
     5941 ttttatatct ttaaatattc tattataatt agagcttttt aaggatgtct ttaaaccttc
     6001 aactacatca tcataaccta tacttatctt tataatattt tttaatttat tgatattttc
     6061 atccttatct atatcttttt tgctaatata gtcaattcct ctaatattaa ttctttgttc
     6121 attgattact gcaacaatat tactactttc tttaagtttc gtaatattta aacttaaatc
     6181 tttttctgtt ttatatggga cagatataat atctattcca tctaagtacc tactcatctt
     6241 ttcttctaat atttttcttg ttttattatc tacaccttct gaaacatgtg atgctataat
     6301 aattgtttta gacagatttt ctttattttg aatttcatta gttccaaaat agtactttct
     6361 cgtgttagtg atagagttgt atacgtcatt taaaggtaat cccatattgg ccttctgaac
     6421 tgcttctaac acagttaatg tcgtcaccat aggaattact cttactccac actttttttc
     6481 tttctttata atttcatcaa aagagtttaa tgaacccata tctgtaaata gtaatacatc
     6541 tttattgtct atattattaa ttaaattttt aaaatctaca ataacttcat catagctttt
     6601 atctaacttc atatcgaaag aaatagcact gcactttcca ataatatcac caactgcttt
     6661 taccatacta cttgctgtag tttctccatg catagcaact ataatattaa tgttattcac
     6721 tattttattt tgttcatctt gtttagcttg tactatgaat aatgtaatat atgctacagt
     6781 ttctatagat aaaaataaat caaattcatt ttcaattatt ccaactattt ttagtgctgt
     6841 tttaaattca cttctgttat ttcttctaat ttgattcaaa tctatgttgt ttgtagagct
     6901 acttttatcc aatacgcttt ttatataaat tgccattgac ttaaatattg attcacttat
     6961 atctatttct tcatcttctt taataatttg tcttattgat tcacacatgc ttttaaactt
     7021 aacattatta aatagagaag catctgctat attcatttct acagatttat taagattagt
     7081 atttactgca ttaataatat ttatatcatt gtagttaata acttcttctt ctcctaatga
     7141 cgtaaataca aaatattcta ttccatgtgt tataacttca tctgctttaa tagacttctc
     7201 tctataatca tcactcactt catttataga tttatcaaaa tattttttct ctatataaac
     7261 tggttcttta ttatctcttt tatatcttag gtatccattt gcaacagcta attttatatc
     7321 attttgaagt tgacctacat tatttttaca atcataaagt aatagtgatt taattatttc
     7381 tttatgtact tttattgaaa tagaaatact ttttgattct tccttgaaaa aatttttgac
     7441 taattccatt ctttcaatca ttgtcctatc tctcaatgat ggtatttcta ttttcattgg
     7501 tattcttcta ttgaatgtat ctaatagtgt tgatgtcgga ttttctgtag tagcacatat
     7561 tattaatacc ttagactctc tttctttcgt tgcatctcct agtcttttaa atgttccctt
     7621 atctataaaa gtaaacaaca tttcttgacc ttctgctggt agtctatgaa tttcatctaa
     7681 aaatagtatc cctccatttg caatttccaa aagacctttt ctggaagatt ctgcacctgt
     7741 ataagctcct tgttctaccc caaaaatatg tcccataagt aattgtgcat tattagcata
     7801 atcagcacaa ttaaatgtta caaaaggagc tttatttttt ctaattccac tatctataga
     7861 ataatcgtac atatatctag caatcatact ctttcctact cctgtttctc catagataat
     7921 agtatgaagt ccatttgtag gatagagcat tgcgaccttg catttttcta ttataggtct
     7981 taaactatgt tctttaccta ttagtgtttc aaaactatct ttttttattt tgttttctac
     8041 ttttgttcct ttttttatgc caagcaactc actttgtgta tcaagcaatt cattcttgtt
     8101 aaggttaaga tttgcaggga tataatacac aggtcttcct tttatcttcg taaccatacc
     8161 ctcattgaat aattggttta aatatgtact tatattgttt cttgcaattc ctgtcttttc
     8221 tgataattgg ttagctgtaa tccctttttc attttcccta tatgatgatt ctaaaacttt
     8281 cattacttta tcttttttat atatcattac aagctcccct acattatagt attttattaa
     8341 ctaaaacccc tatacacact ttatttttta ataaactctt attctttaac taaggttata
     8401 atttatcaaa ctttccttac tttaaattat agaaaattat tgtagaaaaa ataagattat
     8461 tactcatttt ttctacatta ctatttttaa ttttatagta ctttcttaac aacatacttt
     8521 acttgttttt aattttatta tatttgaatt tattatattt gaaaagtaga aataaaaaac
     8581 cctattttgg agtagtttta aaatttctcc aaaaataggg ttttagtttg tatttcaaat
     8641 aaatacctgt tcaagtattt ttatactaaa caacttttaa attttgttgg tatttactta
     8701 tgtctgattt agaagaatat acaaaatgtc catctgaaac ctcaatccaa cttaataact
     8761 tttcatttga acaatcatat ccattagaat tatactctat tctatttctt gacttagcat
     8821 atctagggtc aggaacagga attgcagaaa gtagtgattt tgtatatgga tgaataggat
     8881 tattatacaa ttcatttgaa tctgccaatt ctactaattt tccattatac ataactccta
     8941 tacggtcact aatatgtctc accatagata aatcatgagc tataaataaa caagttaaat
     9001 tcttttcttg ttgcaatttt ttaaataaat ttactatttg tgcttgtata gacacatcca
     9061 aagaagctat aggctcatca gctattataa attctgggtc tactgaaaga gctcgtgcta
     9121 tgccaatacg ctgtctttgt ccacctgaaa aatcagatgg atacttattt gcataacttc
     9181 tatcaagtcc tactagcttt agcatctcat atactttatt cattctatct gttttatttt
     9241 tacagatacc ttgaattttt agtggctctg aaattatatc tgcaattgtc atacgtggat
     9301 ttaatgatga agtagaatct tgaaaaataa tctgcatact tttgtttaca tcttttttta
     9361 taaatttgta cgttttttta tctgatatgc aatttccttt gtaaattact tctccaccag
     9421 tagattcatg tagtcttatt atagttttac cagttgtaga ttttcctgaa cccgactctc
     9481 ctaccaatcc aaatatctca cccttatata tatcaaaact tatatcatca actgctttta
     9541 cagtagtact tttgtccaaa tgaaaatatt gctttaagtt ttttatttcc agtatttttt
     9601 ctctattcat tagaaatcac ccttccacaa tttactctaa ctggtacatc tacttcaggt
     9661 gcatctgggt gcaataacca agttgctgca ctatgtgtat cattaatttt aaacattggt
     9721 ggctcttttt cataatctat cttaagtgca tttttattcc ttattgcaaa agcatctcct
     9781 tttggtgggt tcaataaatt tggaggcatt ccaggtatat tatataaata gtcatcctgt
     9841 gaatctaaag taggtaatga acccaacaac ccccaagtat atggatgtct agggtcataa
     9901 aatatatcct ctacagttcc tatttctaca atctttcctg catacataac tgctattcta
     9961 tcagctatag ttgcaactac tcctaaatca tgagttataa atattattga caaacctatt
    10021 ttatgttgta aatcctttat taaatcaatt atttgagctt gtatagttac atctaaagca
    10081 gttgtaggtt catcagcaat caatacatct ggattacagg caagtgctat agctattact
    10141 atacgttgtc tcattccacc tgaaaaatga tgtggaaatt gtttaaatct tttctcagga
    10201 ttatcaattc caaccaattc aataagctct atggctctct tttttgcttc acttttactt
    10261 ataccttgat gaatgattac agcttctgca atttgctttc ctattgatat agttggattg
    10321 agagatgtca tagggtcttg aaatatcatt gatatattct ttcctcttat atcttccatc
    10381 tctttttcag acttcttaac caaatcactt gatttaagta aaacctctcc atttttaata
    10441 tatccattgt atgggagtat cctcatcaaa ctcttacaca gtacagattt cccacaacca
    10501 gattctccaa ctatagccaa tgtctcacct ttttttagat taaaagatac atttcttaca
    10561 gcctgaactt ctccttcttc tactttaaaa cttacagata gattattaac ctccaataaa
    10621 tgttccaaaa accatcaacc cctatgctat tcaaacctta gggatatatt tttatcccta
    10681 aggtttctaa ttaatttcag tattattcaa ttgtccagtc agctatgttc cagaatatac
    10741 ctacaccatg atgacctaaa actgtatcag gtgttaatcc ttttatgttt ggttttccaa
    10801 catatattgc atctatataa gcaataaatg tataaggcat atccttagtc atttctactt
    10861 ggaattgttt atataacttt aatttttcat ctttatcttc tgtttctctt gctttttgaa
    10921 gtattttatc tatagtagga ttagagtaag cactgtaatt tgcaccttta tctgttccaa
    10981 atactttata tgtatggtca tctgggtcaa atggacttcc ccaacctatt aaatgagcat
    11041 cttgattagc ccaatctgtt tcagttacaa caacagcttt agcatcaaca ccaatttctt
    11101 ttaactgttg tgcacatatt ttagccatat caactctaac ttggtcactc tcacctgctg
    11161 ttatctcgaa agctaactta gtaccttctt tttcatagat tccatcagaa cctaatttcc
    11221 aacctagctt ttcaatttct tgtttagctt tttctggatt atattcaaac ttttctatat
    11281 caggattatt atatggtccc atttgtaaag gtgaatatgc tggtacacca tgtcctaata
    11341 atacactgtc aactattgct tttctatcta ttgcatagct taaagcattt ggtaatcctt
    11401 tagctttttt atctttaaag aatttagaat taaaattgta taatatacct ctatagtcgg
    11461 ctgttttcat tatatttact ttaaaattct tttcgtcttt ttcaaagtta gacatatctt
    11521 taggagttat ttgagctagg tctaattctc ctgattttaa ttgcattgcc ttagccttat
    11581 catctggaac aatcttaaag actactttat ctaatcctgg ttcttttaca aaataatcac
    11641 tattcttaac aagtgttata ctttgacctt tgtcccattt ttcaagttta aatggtccag
    11701 tacctattgg tttttgatta aattcatcag tagctatatc cttaccttcc aaagcatgtt
    11761 ttggtaatac tcctactgtt aaataatcaa gcattgctgt atttggagct tttaaagtaa
    11821 tttttatagt attatcatta accacatcaa tttttgttat atcttcataa tttgatgcta
    11881 tctcagaagc attatctgga ttcattatag tttctattgt aaacttaaca tcatttgctg
    11941 taaatttctc tccatcatgc cacttaacat catctcttaa attaaaagta tatgtatttg
    12001 ttgcctcatc aaatttccag tcctttgcta gacaaggaac taccttatta ttttcatcat
    12061 gagctgttaa tccattaaat attaacgaat ttatttctcc atgttcgtat agtgcaggat
    12121 ttatacttgt atagtcatta cttccataaa caagtacctt tccatcttta ggcgtatctg
    12181 ccttcttatc tttgtctcct ccactagaac aacctgccat tagactaagt atcattacta
    12241 aacttaaaac ttttaacttc tttaatttca tgatgctccc ccttttgctt tttataaaat
    12301 atttttcaaa gctttgaccc catttattta aatagatatt tcaaatacct cagtatttgc
    12361 ttatttagta ctcaaatgct gatttataaa ttactagatt ttttattatt attctttctt
    12421 atataattac ctatatttgt tatacacacc aatgtggtaa ccaaaaatat acccggaatc
    12481 agtataatcc accatctatt tgaaagtaat gcttcctctg acaaagataa catacttccc
    12541 caagaaacaa tttctattgg taaaccaatt cctaaaaagc taagtgtcga ttcagtacca
    12601 atagctgcgc ctatgtttgt tacaaccatg aacattattg atgccacaaa atttggaagt
    12661 aaatgttgct ttaatacata gaagaatcct cctcccatgc tcttagctgc tagtatgtat
    12721 tcgctatttt ttatttgtct aacttctgtt ctcactatct tagatatatt catccaactt
    12781 gttatcccta ttactatgga catagatact ggatttgaat ttccaagtat agcttgtaca
    12841 aatattataa ttaatattga aggtatacta agtatgattt ctgtaaacct catcattgca
    12901 tcatctacaa gttcagatgc tgaaccactt atactaccat aaacaattcc tataacagtt
    12961 gatattacag tggaaaatag tcctataaat aaagatattt ttcctccata ccatatcata
    13021 gaatatatat ctctacccat tgagtctgtt ccaaataaaa aagttttatt tggagctaaa
    13081 ttagaacttg ctaaatccat gtatgttggt tcatgtgtca ttataaaaga tgagaataca
    13141 cttccaatga ctatgattga taaaataata attgatatat atggtaattc tttaattttt
    13201 ttagataagg ttttctttgt ttctatatgt tcatcttctg ctactaaaat ataattttct
    13261 cctacaattt caaaatcact atattgtggc ttcattagta tcacctctat catatttcat
    13321 tctagggtct attttattgt ttataatttg agctaacata ttcccaaata ctactaaagc
    13381 ccctgttatt aagcataaaa ctaataacat attataatca tgatactttg cactttcaaa
    13441 acaaagtgtt cctaacccag gataagaaaa aactttttca actacataag ttcctcctag
    13501 tatatgaggt atcgaaatgg ccattatact aatatatgat ggcattatat ttctcaggca
    13561 atgcttaaaa acaatagttc tattatttaa accttttgct ttacataaaa gcacataatc
    13621 ttctcttatt tcctctagta atttatttct tatcatatat gtgtaatacc atagatggct
    13681 taatataagt actgtaagag gtaagattag atgtgatatc ctacttaata tactggactc
    13741 ttctcccatt gaataagcac cactgcttgg aagtatactt aagtttatac taaatataag
    13801 tatgagtact aaggcaaccc aaaatgatgg aatacagttg gttattgttc ctaatttgca
    13861 tatgattcta tcaatgagct tatcttcatg taatgtacaa aatattccta acaataaggc
    13921 tagcacaaag gtaagtatat atcctgaacc acctaatatt atagtgttta tatagacatc
    13981 atttataaca gaagtaacat tttgtttata tttaaatgaa attccaaatt ccccttttga
    14041 tgcatttgaa atccatttta tatattgact gtatattggt tcatccaaac caagtttttt
    14101 catagcattt tctttttctt gtgtactcat tctttctact ccatccccat agtaagagat
    14161 taatgggtct cctggagcaa gtcttgccat gtaaaaaaca ataaaagata ataaaaacat
    14221 tactaaaata aactgcaaca actttttcaa tatatccttt aacaaacccc aacccccctt
    14281 tgttgcaata aaaaaaacca cataaaagca aacgtaaagt aacgttaaaa ccttcgtggt
    14341 tccattatca ttaaacaaaa ttaaattttc tatatttatc tatgagttaa ttttagcatc
    14401 atttattaac tgtgtaaaca aactttttaa aaattgttaa aatttcatca aattatttta
    14461 tttataccta aaataaaatt tttatattct attcttcatt attttctata aaattataaa
    14521 attttaggtt gactatataa gaaaagtata ttatattagt ttgtatgcaa accatttaag
    14581 aggtgatttt attgaaaagt aattatgact gttttagaat agccatgctc ttaaaagaac
    14641 tttattctaa aactatgtac acagtagaag aaaattttaa agaaaatgga ttaacacacc
    14701 aacagattat agttataaaa ctgatagctc ataatcaaga acttacaata tcacaactat
    14761 gtgatgaaat gtctttagct aaaggtacgg tttcaggcat aatcagcaga ttagaacaga
    14821 ttggatatat agaaaaattc aagaaatcta atgataaaag aaatacatac gttaagttta
    14881 caacaacagg atttgaattt gccacaaatt ttaaaattaa aatgcaagaa agttttgatg
    14941 atatttttaa aaattgtgat gaaaacgaat tgagtgactt agtaaaaaat cttagaaata
    15001 tattggcaaa agtaaaggag agataattat gaattataaa ttaatacttg caatagtatt
    15061 tataacatca gctttaattt tctacactat aggtgtattt ggagaaagaa aggcaaaaat
    15121 tttaaaaaag aaacatgtaa ttatattttg gcttggtttt atatttgata ctttaggaac
    15181 atttacaatg agtaacatag ccaacagcca tacttttgaa gtaaaatctg ctttatcaca
    15241 aaaccttcat agtattacag gtcttcttgc aattgtgctt atgctttttc atgcaagttg
    15301 ggctactttt gtcctataca aagatgatga ggaaaagaaa aaattctttc ataagtttag
    15361 tatagttgta tggactatat ggttagttcc atattttata ggtatgttta ttggtatggc
    15421 tggatagtaa aataaaaagt gtgtaagaaa tactgtaggt aaatttgtat aagacaaatt
    15481 tatctacatc cttctttcac acttttacat atttatttat aatactccat tatttctttc
    15541 atgtttctta atacctatat agaatattta attatttcta tagtatttta ttattttctc
    15601 acactttttc atatatatat tgttttacta ttttttcata tgtatatatg aaatatttca
    15661 ttatttctat aaatgtttta ttatctttct catacttttt catctatata ttgcttcact
    15721 attttttctt atttcttcat atacatatga aatatttcat tatttctata atgttttatt
    15781 atctttctca tactttttca tatatattgc ttcactattt ttttacttct ttattaaata
    15841 cttatgtata acatacattt aataaacgct aactattaaa cacctaatac ttgagttact
    15901 cctggaactt cactcaccaa aacattttca ataatagctt tgattgtcat agtcgctcct
    15961 ggacacccac tacatgcccc ttgtagtcta actaaaacca caccattttc atttacatct
    16021 ataagctcta catctccacc atctctttgt aatactggtt ttatcttttc ctcaagcact
    16081 ttttcaactt tttctctcat agctattccc cccagaaata ttttattacc aaaattatac
    16141 caatggttta aatttttgtc catctaatga tttatcaaat tcaatttttg cttcatcaat
    16201 aattttagta tccataaata catcataagc actacaagcc agtactttag ctgaatttat
    16261 catagcagaa aatcctatat ttgaacctgc tgatgaacat gactgccatg tatgacttgc
    16321 aattccaaca ggccaagctg ccattgcaaa ttgagccact ggtattacat aacttacatc
    16381 tccaacatca gtagaaccag ctagtccttt atctttgtca ccgtcaacta tttccttgtg
    16441 tatatagctt tctacaatag atttatcatt ttgtaatgat gttgctacac ctaatctttc
    16501 ttctctagta agagtatcac acaatttttt tgcaaattct tcttcttcag gagtattttt
    16561 agggcaacca acaaaaacca tattgtcata aactaaatca gtcaaacatt ggtttggtat
    16621 atagtcatat attccatctg taaccttata ctccatttca gtttctgtca tcattgctgc
    16681 tccttgagct acttttatta atctatcaag aacgtgttct gcatcctttg cttttttacc
    16741 tctaataaag taccaacttt cagcaaatcc aggaactaca ttaggtcttc caccaccatt
    16801 agttataaca tagtgcattc ttatagagtc aataacatgt tctctcaaat aattagctcc
    16861 aacattcata atttcaacag catcaagtgc gcttctacca ttatgaggag cttgagctgc
    16921 atgggctgtt atccctttaa atttaaattt tacagataag tttgccagac ttcctcctct
    16981 ccaaggtgca tttatatcaa aaggatgcca tgtaaatgca caatctacat catcaaaaca
    17041 gccatttata cacataactg tctttcctcc accttcttct tcagcaggac aaccaaaata
    17101 ttttattgtt ccattaaatt tattttcttc tattaatttt tttatagaaa caactgctcc
    17161 tacacctgct acacccaaca ggttatgccc acatccatgt ccagcttttc cttctaccaa
    17221 tggctttctt tctgcactta cacattgtga tagttctggt aaagcatcat attctccaag
    17281 tataccaatc acaggtttac cctctccaaa acttgctata aatccagtag gtaaattttc
    17341 tacttcttca attttaaatc cttttgactt taggtattct ttttgaatgc tacttgcata
    17401 cttttcttca aaggctaatt ctggattttc ccatattttc ttagataaat caattaattc
    17461 ttctcttatt tcttcaacat tatttatgat atactctttc ataattttcc tccaaatata
    17521 tgtatttttg atatgcttta cagattatat tatatactaa cattctattt attcacaatt
    17581 ttttgttaat taattttatt catatgctct tctatggagt atatcttatc ttcactttgc
    17641 ctcttcataa atttaatgta ctctatcttc cataaagtag tttcagtttt ctcatttaac
    17701 ataaattcta atatattaat ttctctcatt ctaatatctt cataaaatac tacactaccc
    17761 aatattgcca attctaattc acttaatgat gcaaacaaca ttaaatactc ttctccaaat
    17821 aaacattcag attcaagaat atcgataaag tcttttttag cattgatatt ttcctttatt
    17881 attcttattt tttcaattat agatgtacta tctaatatct cattgaatat ctctctaaac
    17941 gattcatcat ccaatttact ttcatcatca acaatataat cttctaagtg ttcattttta
    18001 tttttactca cagttaccat acttttaaaa gtgttacttt ttattgagct tagcacagat
    18061 tcaataaata tcttttcata ttttttaata tatcttatta catcttcatc ttctatgctt
    18121 aatcttttta ttaatacttc aattgcttcc ataagtagta aagttatttt tttatttatt
    18181 ctaatttctc tgttaatttc ttcaaaatca ttataatcca aattactatc tacttcagtg
    18241 ttaaaactat catctacttc aatcaattca attatattac tttcttcaga gcctataact
    18301 ttatcttcag taatattatt gtacatattt ttaaattgtt tttctaaaaa ttcatactca
    18361 tattttgaaa tttctaaatt agtcacttca tttcctaaaa tagtagaaaa aatacaatta
    18421 ttcataataa gttcaaataa atttacaagt ataacatcac aatctatttt ataagtcatc
    18481 ccatagtcat aaaataattt ttctctatct tttttcttaa ataggttaca aaatctattt
    18541 tctatatcta tactctcaat ataattcttc acataataaa tacccttaac atccatatca
    18601 tctagtgcca atggataatc tatacttact ggaacgtcct gagcttcaaa ttttacaata
    18661 taacttttaa agaaacttga taactccatc aaggtatcat tatatgctat tgaattagta
    18721 tccaattttc tactcatcat tagtttataa aaatctttta gcctaactaa ctcttcctct
    18781 aatatttcaa tgcctccaag gtacactttt ttaacattct tagcagtcaa ctccacaact
    18841 cttttttcta tatctccaag cttatttata tatgcatcaa tagcatacca tattgaaatc
    18901 aacagttttt cggcaacttc tgtttttact gatgtgcttt gaccatttgt atatttttta
    18961 atcaaatcca taagtattag agatatttct tgttgtatat tatatacttc tctttcatct
    19021 atcactttac aatttagaca ttccttaagt agagatatgg aatattggtt ctttattaaa
    19081 ttattttcgt ttatattaat attacttaaa ttatttaaca tacaaaatct ctcctatcat
    19141 atattaaact tctccaaatt taaatttctt tgcaaattcg tctgcttctc ctcttacatt
    19201 gtccatattg cctgaaaatt tttcataaaa ttcacccagt atttctataa cctcatcatc
    19261 acatatcttg tcatcagttt cattttttaa ataataaaaa atttcttgca tatcattaat
    19321 agcctctaaa taattgtcca catttgtata ttgtgatgta tatatatttt ctattaactg
    19381 ttttgtaact tttatatcta attctattct tccatacccc tttaaagtat tatctctcga
    19441 atttattata tcttttacat cagattcaga aagagctaga ccataatctc cagattctat
    19501 attagtttca agcagttcat ttctttctat ttcctttaaa ttctgattta tagaactcaa
    19561 aataataggt gatatcattt ttaaattctc cttttttatt tttcccattg acttttttta
    19621 ttttttatgc tatatttaaa ttagtacata ttattttacc aaccacttat aatagtagtg
    19681 ttaaaagtgg tttttgtcaa ctcaaaatta ctatctcaat taaacttttc aattttctca
    19741 acaaaaaaat accatcattt agatatataa ttttctataa caacttttaa ttcagtagtt
    19801 atttactaaa atctaaacaa tgatatattt attaatttta tttatctttt ttggctctac
    19861 ttctactttt ctttacattt gagcaatttc tacaagaagg tgaacagtat tgagatttag
    19921 aatttgtagg aatataaatt ttaccacagt acttgcattt atttaaagaa gaaggttcac
    19981 ttgtaattgc aaaggcatat atagtctcaa tagctgtttt caaagaatca aactcccatg
    20041 caataattgg tttatctaat tggcttatag aaaatcctat cttttgagga ttaaaccctc
    20101 cagacataat agtaacactt tcagttaata gattagatgt atttttatat acacaaattt
    20161 gattaaaatg cttacacata tctttagcaa aacttacaat ccaatctagt tgttcagtgt
    20221 agaactttga aaatactaaa tctaaaatca atggtctctt tccaaaaaac tttggagatt
    20281 cttctctctt tgatacatct ataccccttc tatacttttt aaaatacaca tcttcatcac
    20341 ttgcaaatgg tataaacatg tttacatatt catctgtact caatatattc tcttttgtaa
    20401 tataattctt ttcaatcatc agtactcttt cttctccaac tatatctcta ttatatacac
    20461 ttgaatatat taaacccaat aatccatact tttttacaaa ttcaagtact tctttcttta
    20521 gtttcatttc ttttgtctct tttgatatat cttgtaatgc ctcttgtcct atgcgaatta
    20581 tatctactat tatatcatct gctacatcaa atgggttata catggtaaac acagcatctt
    20641 ctgttggaac tatatataat tcatcttctt cagtaatttt atattcataa tcactatacc
    20701 taatccaatc acttgcaaaa ttccctatta attctatttt tttattcata ataatcattc
    20761 acacctttca atgtaatata taagttcata ttactattac acttttagtt tattccatat
    20821 tttctacact gtcaactatt ctttttatat ttttagcata ttaaaattta taatagcctt
    20881 tataatggat ttctacagct aaatttcttg tttattgatg actaattcat aattaaattt
    20941 ataaaaacta tttacatata aaattaatct ctttataatt gatatgtaat atatttttac
    21001 acttacatat tacatatcaa ttataaagag aggtatctaa aatcatggat agaaaacgtg
    21061 aattaaaaca attatacaaa gaaatgaaat ttgacacagg tgtattcata ataaaaaatg
    21121 atattactaa aaagatttac cttggaaaaa gtaatgacat aaaaagcaaa tttaactctc
    21181 tcaaatttca gcttggtgct ggctcatgta tgataaaaga attaaataat gaatggaaaa
    21241 aatatggtga aaaagcattt acatttgaag ttttagaact tataaaacat gatgataaca
    21301 aaacagagaa agattattta gaagaactag atattttaga gatggtttgg ttagaaaaat
    21361 taaaggaaga taatacttat gaaatttatt ccatataaaa aataatataa tataagaagg
    21421 ggagatagta tctcaaaata gacatttagt tcttgcagtt ggaaatttgg ctggaatatc
    21481 aaatttaaaa aacctggaaa aaatttatgc acagtatatc cgaaagaaaa ttattttacg
    21541 ttttagttgt tgtttgaaag aaagagaaag aggttgtcga agcgatttta caggagttta
    21601 caccagaatt aagagatatt tacgtacaaa caaaaacagg gaatggataa aagtggttaa
    21661 tgattgatat tcgcagaggt tagcagattt taatttacag aataaatata gtctttgaat
    21721 tatgttaata aaaatagcaa atatgataat tttagttggt agagtttgtt cttatcaaat
    21781 aatataataa tcctataaac agataaggag gaatttcaat gaatttagga aatagtttat
    21841 ttcaggcaag gaagaaacat gggctttcac aggaagatgt agctgaaaaa ctgggggtaa
    21901 gcaggcagac tgtttcaaaa tgggaaacgg atgaaacagt cccagacatt cgtcagtcga
    21961 aaaaattagc aatactttac aatgtgtcat tagatgaatt gattgatttt gatattgaca
    22021 tggaagaaat tcaggaagcg attaataaaa caagtgagga aaccgaagaa aaaatcaact
    22081 ggacaaatgc ctggggaaaa aaatacccta ttcttcttaa atatcagaca gatgttaata
    22141 tttctaatta tgcttataag gttaacatga tgttggacga attaaaacag gaataccaat
    22201 ataatgaact ggaagcaatg ttaattctta aagatatttt atacaatgtc tggaaaaatc
    22261 gaaaaagtaa acgaaagcaa taaatccccg ttttttggaa actaaagtat tgaccttttt
    22321 gaagtaaata tcgttctcaa ttcagggaca gcaggtggaa tgaacccaaa attgaaaata
    22381 tttaatatgg taatatctac ggaagtttgt catcatgatg ttacacaaga tactctgaca
    22441 gaatttcact catggttgaa aaccgtattt tttgaagcaa actgtgaatt gataaaaata
    22501 tctgaaatgg ctgtaaaaga aaccaaccaa gaagaaaccg tattttgggg aagaatttta
    22561 gcaggggaag cttttattac caataaggga cgcaaaaaaa taaatgaaaa agaggcttat
    22621 ctccatttga tagcctcttc ccatatattt tataccatta taaaatgaac aatctactaa
    22681 ttttcaatgt gtatattcat tttactaata acactccatt acttctttac agcatccaaa
    22741 cattgctcta taatactctt atcacttata cctattgctg ttactttact agctccactt
    22801 aatatactct tatcactttt atttgaaact aaaacaacag gagcattttt tgcaagtggt
    22861 gatgcaacta aagcataaac taaatcgtct ccacttgtta tatagaactc ttttgctcca
    22921 ttatagaact tttcaataac atttttatta gtttgatatc tatcaatacc gccaattcta
    22981 gttgctctat atctgctcac tatgtaatca cttaatgtag actctcctcc aatagcatat
    23041 actggaacag gagaaacacc aaaccatggg tctaagtctg tgtcttcttc tgctgaaggt
    23101 atatcttttg tcaatattat aggagctcca tctctataag caacagatgc aacacttact
    23161 gcatcagctt catccttaaa tccatttact aaaaacatct tatcactgtt tttaattttt
    23221 tctatttcac ctgcaatttt ataacttgta tctactctat ctacaccatc aattcttata
    23281 acttgcattc ttctcataag taacattttt tctacatctt tacttataga attttctcca
    23341 cctattacat atatcttagt aactctattc agtctttgaa gcgttgcttc tggtatatca
    23401 tccttattag ttaataaaat agctgcatta tttactcctg caagaccact tgcagcaatt
    23461 ccatctacca ttgtagagtc tgcattgata agaactgctg ttgtaaaatc ttgcttatct
    23521 gaaataagcc cagctgtttc atatttatct actccttgaa tattttcaat cttatctagt
    23581 gcgtttattg atgttggaaa actaacactt aacagtaatg ctgaaatacc taacgccaat
    23641 acctttttac taatcctcat aaattacctc cctaaaacaa atatatttag tattagtata
    23701 atataaaatc atttaaaaat agtgtaaaaa tagttacact tttttataat ttaaataatt
    23761 ttagactaaa cacaattcat ccattaaaat aacttcattt attctatatg ctaagccatc
    23821 ccacgttttt tttgacatat ctacagatgt tttcttattt gagttattca ataaagattg
    23881 tatcttagaa tctattgcat tatacaaatt attttcaaaa tcatataact cttcatcttt
    23941 tggaatacct tctttctcca taaaaggtag agaaacatat tctatctttc cagaattatt
    24001 aatctcacta ccaatccact ctttaacacc aagaatatca gtagtgatta catctgtgcc
    24061 acaagataaa gcttctaaaa ctacaactgg taatccctca taaaatgatg gtaacacaaa
    24121 tatttgtgac ctgttgaata gctcagctaa atctctctgt tctaactttc ctaggaaatt
    24181 cattttaaat ggagacttac ttgccagttt aactatctca ttatatgatt cgatatcgct
    24241 tccagtacct gcaaaattga cttctattaa atctttacta tattttaatt tagatatagc
    24301 ttttataaat ggaatcagtc cctttgattt acatatctta cctgcaaata ctatttttat
    24361 tttatcatcc tttattttat aatttttatt ataaaatata tcatcattat aaccactacc
    24421 aattacaact actttacttt cacttactcc aaaagtcttt ataatatcat atttctgatt
    24481 ttcatgaaga gcaaatatta aatctaattt ccttatattt gctattatgt attctttctc
    24541 taaatcaatg gtatttaact gtcttaaaca agttccatga caaatagaca taacctttat
    24601 atcttttacc atttccctaa caaatgcagt taaaaggtac aaatgatgac atattataag
    24661 ttctggttta aaatcattca tagccttatc tatatttact ttaaattgat atttcaatct
    24721 atttatcatg tctatattta aatctttata tcttgtactt tcatatggca tagaatcact
    24781 catacctaca acaggaaaat ttaactcccc acaattatac tttactggat aaaaacttac
    24841 ttcacttgga aaacaattta catcatcatt tacatctata cctgcaatta cagcttgctt
    24901 atgaccgatt ttttcgaacc cttttatcat accagacaaa tatataccac tacctgtact
    24961 atttggtttt tgtgttatta tatgaagaat tttcactttt cacttgcctc ctttctaaaa
    25021 ttaaatagtt ttatacagtc taatcattat atatatctaa atataaacaa gatgaatcat
    25081 cttttacttt aaatcttggc actttaaccg ctttatagtc attatcttca aaatccctta
    25141 ctttattatg tatatatcct ataccaaatt tttctgctat acgtatcaat tcttcttctt
    25201 ttattagatt atatctatca catgcacttg taaaaccatc actcgttagc atcaattgaa
    25261 aatctttttt tatttcaatg tatccatata tagaattttc cacagcttcc tcatcaaaat
    25321 ccaatatcca atagccctct tttgtatttt tcttcaatct attgcttact attgtatcca
    25381 taacactagt tctaatctca tcaaaagcca tctcttccaa gttagaaagt ttactcattt
    25441 catcaaatac agtattatca aatttacaaa tactcctatc ttttataact ttggaatctc
    25501 catttttaat aaacaaagca caatctccta ataaaaaata ctctacttta ttttcatgaa
    25561 attttacaac aactatagaa ctagatggta aatctaattt ttcaacctta aacccattta
    25621 atcttagttc atactctttt tttacttcta ttattccagt attaataata ctctttaatg
    25681 attcttcttt atttatattt ttatataaat acctattcca ccactgaaca taccaccttg
    25741 cgtcactttc agttgacact aaattttttc cattaagtcc tgtagcacca tctaaaaccc
    25801 atgccccaaa tggatttata ccgactacat cttcattaaa tttagctgtt ccttgattac
    25861 ataataggct atagtttctt atatttaaag ccatatcaac ctccaaattg aatatctaaa
    25921 aataaagttc tatttaaaat tatacaacaa ataaaaaaat ctacaacaaa acttgttgta
    25981 gatttttact taatctatat tatctttata ctttcagtat tttaatgatg gaataatcta
    26041 tttttagtaa atgccatgac tatgttatac ttattacaag ttaatataac attatcatca
    26101 cgaatagaac cacctggttg tgctataaaa cttaccccac ttctttttgc acgttctata
    26161 ttatctccaa atgggaaaaa tgcatctgaa ccaagtgcta ctcctgttaa agttttaagc
    26221 catgctcttt ttccttctcc agttagaggc tctggttttt cttcaaaaaa gttttgccat
    26281 atgccatcag caagcacatc catataatca tcagaaagat atacatcaat agtgttatct
    26341 ctatctggac gacctatatc ctttttaaat tttaagttta atactttagg atgttgacga
    26401 agataccaag tatcagcttt gttacctgca agtctagtac aatgaatacg tgattgttga
    26461 cctgcaccta cccctattac ttgtccatct ttagcataac aaacagaatt tgattgtgta
    26521 tattttaatg taataagagc tataattaaa tcacgttttg cactatctgt aaaaatttta
    26581 ttatcagttg gaatatcttt taataggtct tcatttatta atatttcatt tctaccttgt
    26641 tcaaaagtaa ttccataaac atcctttgtt tcaataggtt caggagtata attttcatct
    26701 atttttacaa tattatagtt gcctttacgc tttcctttta atatttcaag ggcttcatct
    26761 gtatatcctg gagctattat accatcagaa acctctcttg ctataatagt tgcagtctcc
    26821 ttatcacata catctgaaag tgctataaaa tcgccataag aagacattct atctgctcct
    26881 cttgccattg cataagcaca agctaatgga gaaagctcta aatcatcaac aaaatatatt
    26941 tgctttaaag tatctgataa aggtacccct actgctgctc ctgctggact tacatgttta
    27001 aatgaagttg ctgctggaag acctgtagct tcttttaatt ccttcacaag ttgccaacta
    27061 ttaaatgcat ctaaaaaatt tatgtatcca ggttttccat ttaatacctc tataggaagt
    27121 tctccattct tcatataaat ctttgatggt ttttgatttg gattacagcc atactttaat
    27181 tctagttctc ttgccatata ctcatccctc tctataataa attaatcata agtttgatta
    27241 atcctatatt aattaaatta ttcgtactaa taaaattata tggcatattt atccattaat
    27301 caactatatt ttttaaaact cctattcctt cgactttaca ttcaactata tcgccatgct
    27361 ttaaatatac ttgaggattc atccccatag caacccctgc tggagttcca gttgaaatta
    27421 tatctcctgg ctttagagtc attcctcttg ataaatcatt tattatagta ggtatatcaa
    27481 atataaagta ctctgtatta gaatcttgtc ttacttctcc atttactaca ctacttatat
    27541 ctaattttaa aggatgttca aattcttctt tatgtactat acaaggtccc atagatgtat
    27601 gtgtgtctaa gctttttcct ctaaaccatt gaacatgttt atcttgaagt gctctttcac
    27661 ttatatcatt tactattgta tatccaaata tatattcata agcatcttct tttgatatat
    27721 ttataccttc tttacctatg attactgcaa gttctgattc atagtccaac ttctctacca
    27781 aatctccatg taagcttatc ttatcatcca ctccaattat tttgtctgct ctcttagaaa
    27841 aataaattgg ataatctggc ataactacgt tttttataac tccatctggg atttcattga
    27901 catggtcttt atagtttaga cctaaacata taatatcttt tttaggtctt acaattgggg
    27961 aacataattt aacttcttct aacttataac actttgcgtt ttcaaatgaa ttgtttttta
    28021 ttttttctaa atctcctgta gatacgtttt caataaaatc attcatatca gtatataatt
    28081 tacttaatcc taaactatta atttcatata tactatttgt ttctgaatta aatacaccta
    28141 ttttttcttc attgctacta caaaaagtca caaatttcat ttttattcct ccattcaata
    28201 agttgattaa tattatttaa tttttattag tttatataaa ttgtaattca taatatttat
    28261 atgtcaatac atatttatat attttaaaaa tattcaaata attt
//
LOCUS       DAELJO010000029        28110 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELJO010000029 DAELJO010000000
VERSION     DAELJO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.029"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(178..693)
                     /locus_tag="KPB88_001138"
     CDS             complement(178..693)
                     /locus_tag="KPB88_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG0053435.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1056..2126)
                     /gene="leuB"
                     /locus_tag="KPB88_001139"
     CDS             complement(1056..2126)
                     /gene="leuB"
                     /locus_tag="KPB88_001139"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG0053436.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2133..2624)
                     /gene="leuD"
                     /locus_tag="KPB88_001140"
     CDS             complement(2133..2624)
                     /gene="leuD"
                     /locus_tag="KPB88_001140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG0053437.1"
                     /translation="MIGNGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2639..3916)
                     /gene="leuC"
                     /locus_tag="KPB88_001141"
     CDS             complement(2639..3916)
                     /gene="leuC"
                     /locus_tag="KPB88_001141"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG0053438.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3873..5615)
                     /gene="leuA"
                     /locus_tag="KPB88_001142"
     CDS             complement(3873..5615)
                     /gene="leuA"
                     /locus_tag="KPB88_001142"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG0053439.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RSHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5739..5985)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6133..6579)
                     /locus_tag="KPB88_001143"
     CDS             complement(6133..6579)
                     /locus_tag="KPB88_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053440.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6600..7319)
                     /locus_tag="KPB88_001144"
     CDS             complement(6600..7319)
                     /locus_tag="KPB88_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0053441.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPRKVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7437..8711)
                     /locus_tag="KPB88_001145"
     CDS             complement(7437..8711)
                     /locus_tag="KPB88_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG0053442.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8911..10278)
                     /locus_tag="KPB88_001146"
     CDS             complement(8911..10278)
                     /locus_tag="KPB88_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0053443.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10528..10992)
                     /locus_tag="KPB88_001147"
     CDS             complement(10528..10992)
                     /locus_tag="KPB88_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG0053444.1"
                     /translation="MSLRIGGNSLGYLHILILSMFPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10997..11626)
                     /locus_tag="KPB88_001148"
     CDS             complement(10997..11626)
                     /locus_tag="KPB88_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0053445.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11660..12547)
                     /locus_tag="KPB88_001149"
     CDS             complement(11660..12547)
                     /locus_tag="KPB88_001149"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG0053446.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12576..13814)
                     /locus_tag="KPB88_001150"
     CDS             complement(12576..13814)
                     /locus_tag="KPB88_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053447.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14198..15340)
                     /locus_tag="KPB88_001151"
     CDS             complement(14198..15340)
                     /locus_tag="KPB88_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0053448.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15481..16347)
                     /locus_tag="KPB88_001152"
     CDS             complement(15481..16347)
                     /locus_tag="KPB88_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0053449.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16681..18228)
                     /locus_tag="KPB88_001153"
     CDS             complement(16681..18228)
                     /locus_tag="KPB88_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0053450.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18221..19357)
                     /locus_tag="KPB88_001154"
     CDS             complement(18221..19357)
                     /locus_tag="KPB88_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053451.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLAVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19620..20717)
                     /gene="dinB"
                     /locus_tag="KPB88_001155"
     CDS             complement(19620..20717)
                     /gene="dinB"
                     /locus_tag="KPB88_001155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG0053452.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDYRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQL"
     gene            complement(21107..22612)
                     /locus_tag="KPB88_001156"
     CDS             complement(21107..22612)
                     /locus_tag="KPB88_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG0053453.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22873..23223)
                     /locus_tag="KPB88_001157"
     CDS             complement(22873..23223)
                     /locus_tag="KPB88_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053454.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23446..23637)
                     /locus_tag="KPB88_001158"
     CDS             complement(23446..23637)
                     /locus_tag="KPB88_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053455.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23755..24669)
                     /locus_tag="KPB88_001159"
     CDS             complement(23755..24669)
                     /locus_tag="KPB88_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0053456.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERFNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(24803..26299)
                     /locus_tag="KPB88_001160"
     CDS             complement(24803..26299)
                     /locus_tag="KPB88_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0053457.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKSTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(26579..28069)
                     /locus_tag="KPB88_001161"
     CDS             complement(26579..28069)
                     /locus_tag="KPB88_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0053458.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9185 a         5136 c         2880 g        10909 t
ORIGIN      
        1 aaaaaatata ctttttatct atataaatta atttttttag tatgtaaatt ttacgctatt
       61 acgataaatc tttttagtat aactaattta tttttatctt gtatttttat tgtatttttg
      121 aattttattt tttctaaata taaaatttat gttttaaaat ataaaatttt tatttaatca
      181 aagttcaata tattccactt tttctttcga ttcttcaaaa aaccattctt cattagaaaa
      241 atcaccttct gcaatttgtc caataggcat tttcatatac tttccagttg ctgtcactgc
      301 tatatctcca tttggtagaa ttatttctcc tgtaccttca aataattttc tgttttcttt
      361 agttattctt ccaacaactt ttatagtagc atctgttggt actggtttct tatatttcaa
      421 ctctaaagat actgttactc cccatatttg gtcatcattt atacttacag ctcttccaat
      481 agtctcatcc aatattgcag cagacatacc tccatgaact ctcccaggat aactttgatg
      541 ccaatccttt gtattacaaa ttgatacaag ctctccattt tcaagttcat aaaaatctgc
      601 tttaagtcct agctcatttt gtgttccaca aactaaacaa gatttactac tattttgctt
      661 cttcaaaact ttatactttg ccaaaaaatc catctcctaa ccgacattag tagtcgctta
      721 atattaaata attctaaaat atattatata atattaataa aatccctgca aacttctaat
      781 aataaaaata ggctacttca atatgataag tttaatttaa agtattattt tccaaaaaat
      841 aactttatca aaatagaaac tcaaagtaga tataaacctc tttttcgtta ttaaaatagt
      901 aaaaatatat ttgacttaat ataaaaaaga gtgtctcatt aactaataaa gtttattttg
      961 agacacccct cataaaacaa attatactac aatagatttt tactaattct acaagttata
     1021 tatttaaatt atattattca aattaaacta taattctaaa tttcatttac tattaattcg
     1081 cccatttccc ttgttcctac aacatttccc acaccattat agatatcaat agttctataa
     1141 cctttattga gtacactttg tacagcatct tcaatacact tagcttcttc ttctaagtta
     1201 aaactatgtc ttaacatcat gcttacagat agaatagtcg caattggatt tacaatatct
     1261 tttccagcta tatcaggtgc acttccatgt attggttcat acataccaaa actatcttct
     1321 ctaagtgatg ctgatggtaa catacctata gaacccgtaa tcatagatgc ttcatcagat
     1381 attatatctc caaatatatt attagtcact attacatcaa attggctagg gtcttttaca
     1441 acttgcatag cagcattatc aacatacata tgtgacagtt ctacatcttc aaactcttta
     1501 gctaagtctt ccattatact tctccaaagt cttgaactct caagcacatt tgccttatct
     1561 acacttataa gttttttatt tctatttctg gctatttcaa aagctctctt accaattctt
     1621 cttatttcat tttcgttata tatttctaca tcataagcat tttcgatacc atcaatgatt
     1681 tttcttccac gttctccaaa ataaatgccc cctgttaatt cccttacaac aacaaaatca
     1741 atgcctttct caactatatc cgttctaagt ggtgaagctt cttttatact ttcatacatt
     1801 gtagctggtc ttaaatttgc aaataaaccc aatgactctc ttaattttaa aagaccagac
     1861 tctggtcttt tatctccctt acatttatcc cactttggtc ctccaacagc accaagcaat
     1921 acagcttcgt tttttcgaca agtttcaaca gttttattag gtagaggttc tccagtttca
     1981 tcaatcgcgc atcctccagc taatacatat tcacaatcga atctatgatt aaatttacaa
     2041 caaattttat ttagaacctt tataccttca tctataattt ccggtccaac accatcacct
     2101 tttatgactg ctatattaca attcatactc cactaccccc tatttgcttt tattgagtta
     2161 attaagcctt catttaatat aatattttgc ataaactcag gaaatggttc tgctttatat
     2221 tcttttcctt ttgttatatt ttttatcaca cctgtagaaa aatctacttc tatattatcc
     2281 ccttcatcta tgttattggc agcttcttca cattcaagta taggaagccc tatatttata
     2341 gagtttctaa aaaatattct agcaaaagta gaagcaatca cacaggatac tccactttct
     2401 tttattacaa ttggtgcatg ttctcttgat gaaccacaac caaagttttt agttgcaact
     2461 ataatatctc ctttttttac cttagaaata aatttatcat caatatcctc catacaatgc
     2521 gttgctagtt ctttataatc agctatattt aaatatctag ctggtattat tacatctgta
     2581 tctatattat caccgaattt aaaaacactt ccattgccaa tcataaattt tacctccctt
     2641 aaattatttc tgatggtttt gatatttttc cagttatagc tgatgctgct gctacagctg
     2701 ggcttgcaag atacacctct gaatcaacat gccccattct tcctacgaaa ttcctatttg
     2761 ttgttgaaat ggatttttct cctgctgcaa gtatacccat atgtccccct agacatggac
     2821 cacaagttgg tgtagaaact gctgctcctg cctctataaa agtggtaata tacccttttt
     2881 ctatagcttc aagatatact ttttgagttc cagggaatat cataactcta actccatctg
     2941 ctactttttt gcctttcatt atctcagcta ctacttcaag gtctgatatt cttccatttg
     3001 tacaagagcc tattacaact tggtctatct ttatatcctt atctatctca tctactgttc
     3061 tagtattttc aggaagatga ggaaaggcta ctgtttcttt tagttcagct aaatctattt
     3121 cgtatacttc atcatagact gcatcttcat ctgcttcata tatatttaca tcttttgtca
     3181 tagttgaaca cttatgtgaa tttatatatt ccatagtctt atcgtctact ggaaaaattc
     3241 cattttttgc tcctgcttct attgccatat tacaaatagt aaatctatca tccatagata
     3301 agtattctac tccatctcca caaaattcca ttgacttgta aagtgctcca tcaacaccta
     3361 tctcccctat tatatgaagt attatatctt taccacttat ccatttattt ggtttattct
     3421 tcaaaacaaa ttttattgct ccaggaacct taaaccaaca ttttcctgta gccatacctg
     3481 ctcccatatc agttgaacct acaccagtag aaaatgctcc taatgctcca tatgtgcaag
     3541 tatgagagtc agcacctata attacatctc cagcagttac tatacctttt tcaggaagaa
     3601 gaacatgctc tattcccata tctcctacat caaaaaaatt tacaactcct tttgattttg
     3661 cgaaacttct tgtaaattta cattgttctg cactctttat atctttatta ggtgtaaagt
     3721 ggtctaaaac catcactact ttagtcttat cataaacatc ttcaattcct aatttttcaa
     3781 attctctaat ggctactggt gatgttatat cattacctaa aactacatct aaatctgctt
     3841 cgattaattg ccctttctta acttcattta atttagcatg tttggctaat attttctgcg
     3901 tcatagtcat ccccattttt tatttcctcc tcaatataac ttttatttat tgcattcata
     3961 agtgcattaa tagctgctgt atttatgttt tccgatatac ttacaccaaa atagacttca
     4021 ttattgtgct ctattcctac ataagctacc cctcttgaat gtgacccttc ctctaatgca
     4081 tgttcccagt aatatctaaa tttataatct ttcatgccac attgctttag tgcattatta
     4141 aatgaatcta caggtccatt tcctatacca acaatccttt gttcatttcc catataactt
     4201 attcttgcag tcatctcaat catattagta tcattagaat catcattttc atagtttaat
     4261 tcatccattg attttatttt atacttctta actttataag ggctctcaac ttcaatatat
     4321 tctttcttaa acaaattaaa tattgcttca ctactaagtt cttccccaat tctatcagat
     4381 tccattttga ctacatttcc aaaatcagaa tgcatatttt taggtatcat aaatccataa
     4441 tctgtttcca ttatataaac agctcctcct tttccagatt ggctatttat ccttataatt
     4501 tctttgtatt ctcttccaat atcatgaggg tctattggta aatatggaac ttcccagtaa
     4561 tcattatctc tgtttttcat tgctatcatg ccttttctaa tagcatcttg atgtgaacct
     4621 gaaaaagcac aatgtacaag attacctgca tatggatgtc tatcatgtac cataagtttt
     4681 gtacagtctt catatatacc tattatctta tctatattgc taaaatctag ctctgggtct
     4741 attccttgtg tataaagatt aagtcccaca tttactatat ccatattccc agttctttca
     4801 ccatttccaa atagtgttcc ttcaagtcta tcggcaccag ccaataatcc aagttcactt
     4861 gcagccgtac aagttcctct atcattgtga gtatgcaaac ttagtataat agaatcccta
     4921 caacttatat ttttacaaaa ccattctatt tggtctgcat atacgtttgg tgttgccatt
     4981 tcaactgttg atggaagatt tattattact tttttttgag gagttggttt ccaaacatct
     5041 atcactgcct cacatatttc aagtgcataa tccatctctg ttcctgtaaa actctctggt
     5101 gaatattcaa aagtaaattc tgtttccgga tattttttta cttcttcatt aaataatttt
     5161 gctccttcaa ctgctatatc tattatttct tgtttactca tattaaaaac cacatctctt
     5221 tgaagaacag atgtagagtt atataaatgt ataattgctt ttttacatcc ccttattgat
     5281 tcaaatgttt tttctataag atgagaccta gattgtgtaa gtacttgtat agttacatca
     5341 tctggaatca tattctcatc tattaatttt cttaaaaatg tatactctgt atttgatgct
     5401 gatggaaatc ctacctctat ttctttaaat cctgtatcaa taagcatttt gaacattctg
     5461 actttttcat ttacactcat aggtgttggc agtgactgat ttccgtctct taaatcgaca
     5521 ctacaccaaa ttggagcctt aaatatttca ttatctggcc atttacgatt tactatttgc
     5581 attttatcat attttttata ctttccacac ttcatgttat ttcccccttt ttttgttttt
     5641 tgtattcagt atacctcata tcgacgcaat cctgcacaat tcataaggat aaatgtcaat
     5701 tttatcatta gaataaaaaa aacctcatct ctaataaaat tagagacgag gttttgctcg
     5761 cggtaccact ctatttagct ttctaaaaag ctcccttagt aatagccaat acaataatta
     5821 taatgactat atttcctata acgtggaatt actccgatta ttctaagatt taaatcttag
     5881 aaaacagctc ctagacaagt tcagaactat caactactaa atcacaccat cctttagctc
     5941 tctgaaagtt tcattgttct tactacttct attcatcact tttttaatca tttaaatttt
     6001 ctaaatattt tgatttaata gttattatat acacattaaa atgtattgtc aacacaaaaa
     6061 ataattatct caatttttta tttttgataa gcaattctat aaaacatgct aactattgaa
     6121 aacagtatga tattatgcct taaacttttt catcttttca tctatgatat tcaataattt
     6181 tttttcatct tccaaactca atgcccttaa tctaatttta taattagatt cgtcagtgta
     6241 tatatttaat atatttttta ctacttctat tttttctatt atcttataat taaacttttg
     6301 actttgttta gattttatac tgaatataca ttgattattt attaatattg aatgattatc
     6361 gagagtaata tatacttttc cctcatattt atttgaaaaa tcacgacctt gtttaaatag
     6421 aaaaactgct gttattatac ataaaatata taatattgtc gttgaatata taaaaattgt
     6481 agctaaaatt ataagtgcta aaaccatata tatagtcatt ccttttttaa aataaccatt
     6541 gtcataataa ttcatataga taaacttatt tttactcaaa ttttttcact cccaagattc
     6601 taaaaattta tatcaaaaaa ttctataatt tcatcttcat gataatttat gactaattca
     6661 tctggaaaat ctactgcttc taaaactttt tctgcatttt caaattctcc tatagaataa
     6721 catatatgag agtcagtacc aagaattact cttgcaccat atgttttaca caatgtcaac
     6781 atatgactta cattttccca agtacccgtt ctatgagagt ttgaacttaa tgatgaatta
     6841 tttatttcta aaaggatatt tttatcttta gcttttttta ctataggttc ataatctaaa
     6901 ggatacctac tatcatctgg atgtccaatt atttttacct taggtttatc cataactttt
     6961 aaaatagcat ttgtattttc ttcttttgta ccagaagcta tacaaggtct atgcaaactt
     7021 gcgattatat aatccaaatg ttgcaacata tcttcttgaa catctaaatt accattataa
     7081 tccattatat ttccttctat tcccctaaga atctttacgc cttgcacttt acgtggaagc
     7141 aaatgcaaat tataaaaata gaatggatgt ggtcctcctg gcatattagg tccatggtct
     7201 gatattccta agtattttat attcttctta ctcgcctctt cgacattttc cttgaaagta
     7261 ctatgtgcat gtccacttat tagagtgtga caatgtacat ccataataga ctttttcatt
     7321 aaatttcctc ctaatatttt acataaattc gtgaaaatag ctaaagggct atttcaaaac
     7381 taaagctgtc ataatagaca gtgtaacgtt ttaaaacagc cccattttca attattttac
     7441 acttctaaat ctttaaattt atctttaaat aattctccaa tacttcctaa agattcctct
     7501 acttctaggt attctgttat atcctcttct ggctctctag ttgcttcttt tattgaaacc
     7561 aacattcttt tagtctttgg tttaaaatca agaatcttaa ctttaacctc atctccaata
     7621 ttaacaacat tagtaacttt agccactcta gtatcactca attcagatat tggtaaatac
     7681 gcatctacac cttctttaat ttgaacaaaa gccccttttt ctattattct aagaacttta
     7741 gcattgacaa catcatcaat ttgtacttct tttgctatta aatcccaagg gtcattattt
     7801 atgtctttta atgccaaggc aattttttta gtctctttat ttacatccaa cacataaact
     7861 tctacttctt gtccctctga aacaacatct tcaacacttt caactcttgt ccaagctaaa
     7921 ttatttatat gtgctaatcc ttcaatccca ccaatatcca caaatgcacc ataaggcatt
     7981 atctttgtca ccttaccatc tcttttttgt ccaacttcaa gagaattaaa taagtcttct
     8041 ttagctgctt tgatacgagc cttttcttct tctctttctt gcttaattct tgctctttca
     8101 gcttcttttt ctgccttaat ttgagctctt ctttcttctc tctctttatt tatacgctct
     8161 tgtaatacat ctctatgaga tgctacaagt ctattttttc taacatccaa ttcttttata
     8221 taaacttcta agttaagacc aacaaattta gatgtatctg taataaattt tgtatcaagt
     8281 tgagaaattg gtataaaaaa tgtatatgta ttgtaattag caaatacacc tttatctata
     8341 ttcttttcaa catttacagt tattattcta tgttcatcat aagccttttg taactcggca
     8401 aaatcatttc ttttgtcaag ttgtaattta gataaagtta ttgtaccatc tttttgagat
     8461 acttttgtta ttacagcagt tatttcatca cctatgtggt atatatcttc tatatattga
     8521 tttttttcta tgttcaattc acttataggt attattccat caaacccata gtctaatcct
     8581 aattgtactt catcatttct cactgcagtt atttttccag ttgtcaattc tccaactttc
     8641 actttgctga atacttgttc ttgctggtca agtaattctt gcattgttaa atcattttcc
     8701 attataaaca tcctttcctc ttattgatta tcatatctgt aaatattata ccattaatat
     8761 tgcaaatact tacatttttc ttactataat ttttaaaata aattcaactt catatattta
     8821 tacattatag aaaatatctt attaaattaa aatacaaatt ctaattttta tctaataaaa
     8881 tattttcata aaaaatttat attgtatatc ttattctttt attgctttag gttttgatag
     8941 aaaacattta taaaatataa agaaagaaat tgctgttata gtagcagctg ttatatctgc
     9001 aactggctct gctaaaatta ctgcaaaaag tttttcatta aagaatgttg gaagtataaa
     9061 tattaaaggt attaaaagta caatctttct aagtagagct aaaaccaaag atattgttgc
     9121 ttttccaagt gctaaaaatg tctgttgaca agctatttga atcccaaaca taaaagcacc
     9181 agcaaaatat atttttatac tccaactagt catactaagt aactgcggat tcttattaaa
     9241 tatactaaca aaaaatgcag gaaatatcat taataaaagc cataaaatag tagaatatgt
     9301 aaaacatgac aataaacaca acttaaatgt cttttttact ctatcaagtt gacctgaacc
     9361 ataattataa cttattattg gttgtgctcc ttgtgtaacc cccatattag gaagcataag
     9421 aatttgcatt atactgctca ttatggccat tgaacctact gctaaatctc ctccatacat
     9481 ttgtaattta ctatttaagg ctataagtac taaactttct gtagattgca ttataaatgg
     9541 agagattccc aagccaagca ctggtaagat gatacttgct tttggtatca tatacttctt
     9601 ttttatttta agtatacttt tctttccaaa gagaaacata agtacccaaa tagcagaaac
     9661 gccttgtgca attatagtag ctaatgcagc tccttttaca cccatattaa agccaaatat
     9721 aaaaatcggg tcaagtatta tgtttattaa agcacctata gcaactgtca tcattccagt
     9781 tgtagcaaac ccttgggtat ttataaaagt attcataccc attgaaatct gaacaaacac
     9841 tgtacctatt aaataaatat ttaaatagtc cattgaatat ccaattgtag attcacttgc
     9901 tccaaacatc caaagtaaag gctcttttcc aatcttaaaa cctatagtca aaagtattgc
     9961 taaaatcacc agtacagaaa aactgttact cattattttc tctgcctcat cattgttttg
    10021 ctcacccatt tttatagcag ctaatggacc tcctcccata ccgattaaat aactacatgc
    10081 agttattata attattattg gaaatgccac tccaacacca gccatagcaa cttctccatt
    10141 ttccattcta ccaataaaaa tcctatcgac tatgttgtat aaaacattta caatttgtgc
    10201 gataatagca ggtgtagcaa gtttaaataa taatttaccc acactttcac tacctaagtt
    10261 agtgtttgaa ttttccattt taaaacctcc tcaatccaat aattaaaatg tatcactaaa
    10321 attttattct atataaaaat ttcattttat tagcagacaa atttagagta ctttccctac
    10381 aagtaatact atactatatt tcttttgcca tagtaaacat tttagaacta actgaagtta
    10441 aatactttat ctcatttttt atttattatt aaaaattttt cacatatact cataaaaaaa
    10501 ctatccaagt atattactta gatagtttta tattatatgt aaagaaagag ctgaaactat
    10561 aacccctgat aacaaattgc caagaaatac tcccataaaa gcatctttta ttctcatttt
    10621 taatatggaa gctatagcag atgcagtcca tgttcctgta gttggcagtg gtatacctac
    10681 aaatagtatt ataccaatta tcgaaacact ttttaatttc ttcattcttg agtttatttt
    10741 tctatcaatt acatcagcaa tccctttaaa tagtttcttc cctcttaaaa attgtaatac
    10801 atgtctgaat gtcaatatca agggaatcga taccaatgta gaacctatta cactaaatac
    10861 ataaacccat ataggattta agcccattgc aatacctata ggaatggctc ccctcaattc
    10921 tgttacagga aacatagata aaattaatat gtgtaaatat cccaaactat tccctcctat
    10981 tctaagtgac attaagttat gaatttattt gctctataac ctcaccatcc tttttactaa
    11041 ctaaaaatgc atttttagtc aatgaaagca ttggtaaatc actcttagag tctgaatatg
    11101 catatgaatt ttcataatca atctctatcc caagtttacc taatacagcc tttattcttt
    11161 ttactttttc aattccctta cagttttctc caatgaattg atttttatat cttgaatttt
    11221 cataaaaaaa ctccgtgcct ataacctcgt ctgcaagacc ataatactta aaatatttca
    11281 tatatgcata tggagatgca gttatcataa ttataacatt atcttcttct ttatttttgt
    11341 aaataacctt tttcatagat tcgttaattt ttttcattat gcaatcatca aaaaactttt
    11401 ttaaatcttc ttcactaaaa tctactattg gataaaagat tgcctctttt gccttcttca
    11461 aaggtatcat tctaattaaa tacataaata gaataaaaat aatgtatggc aactttataa
    11521 tagttttaaa ggttttattt tttaaagagt aaataaaaaa atctatcatg ctatcagtat
    11581 ttataacagt ataatctaca tcaagtaata atacactttt tttcataagt atttccccat
    11641 cctatcacta aaaatatcac taaaaaatca atattattaa acatgtaatt atccataaga
    11701 ttacgtttat ttttaaagca ttatctttta ggacaatatc agtaggatta ttttcatttg
    11761 tagtgttaat tatataatta tatctaagta caccataaac tacaaatatt gttgtaaata
    11821 tcatatgtgg cttttctgga ttacttgtag aatacaatgc ataacataca attgtagaac
    11881 ttaatataac tatcatcatt tgatttagat tttctatgtc ataatcttcc aatatttttc
    11941 tatgttcaac tgcatcatct cttagagttt ctatttcttt tttacgtttt ccaagagcta
    12001 agtacagaga taagaaaaat gtacataaaa ttatccaatt tgataaacta acacttatgg
    12061 ctacacaacc tgcataaacc ctaagtataa aaccaagaga tattgaaaac acatctaata
    12121 gtattatatt ctttagttta agagaatata atatattgtt tatcaaatag agactgagaa
    12181 ttatagaaag attaaaacta acaactgttg ataaacagac tgaaattaaa gctaatacta
    12241 caaatagtat attagcactt tttttgctta ttcttccact tgccactggt ctattacatt
    12301 ttttaggatg ttttttatct ttatccatgt ctatagcatc attcagtgca tatactgatg
    12361 atgacattag gcaaaaacac acaaacgcaa taaacgtatt taaaaataaa tttatattta
    12421 gtatgttgtt tgaaaaaatt atagctgaaa atacaaagaa attctttata tattgcttta
    12481 ttctcattaa ctttatataa tctgaaattt tagtaagaag ccttatactt gtattggtct
    12541 gtttcataaa cttactaccc tttcaatatt attttttaaa caggatatga ttttatccct
    12601 atgtttaaaa tttctataaa tccattgaca ttactaatta ctatagtaac caataacagc
    12661 gatagaaata tattatacat attcttttca ttttttctaa gagctttcgt aaagtaagcc
    12721 aataaaatta taactgcaaa actaaaattt atagccataa tacttgaatt gactagatta
    12781 tatcctacta taccatgtaa agttagattg aatataatta ttcccataca tagcttagga
    12841 actctttttt ctgaattttc aataactgat aaaacacaaa aacataaagc taataatgta
    12901 agaattactg gtattgtagc aacttctcca ttttgcaaaa ataacccttt tttaatactt
    12961 gctccaggaa aataaaaaac cgaatacaat aatctctcta taatcattgc aatcttttcc
    13021 aaaattggtt ctccacttat aaatttcgta tcatactgta ttacatttcc aaggttcata
    13081 aaactctcat aagacttcgt tgaaactaca caaatatatg ccattaggat aaatgtaatt
    13141 aaatgtttta acatttttcc tatttttaat ttaaactcaa atatcatatt aaaaaaatac
    13201 aaatatatat tactaatagt tattccaaat agaggaatag ctgctaattc taccaaataa
    13261 gaattttttc ctttcatctt atttgcaata actatgaaaa atattaatga aaattggcta
    13321 tatataaatc tctctacatt taaagtcata aataactgtg gaaaagaaaa tatcattatc
    13381 atagtaagta aaattaaagt taatctctgc atttttatct ttttaatatg attaaagact
    13441 aaagttattg acatagcact agttattatt tgtagacaaa ttagtccaaa cccataggca
    13501 ctattaaaat ctgttgtccc aggatttgct attgaagtac aaaataggta tattggatat
    13561 gcaaaaattg atataattgc tgaaaacaat atatgcttac tattgtcttg acttatagca
    13621 aatacattta aattgaataa aactcctgta tctgtatcta acaatacatc ataagtattg
    13681 tttaaatcat aaaatatagt agtcttagaa aaaaatatct tagttattat taagtaagtt
    13741 attacaacta atataaacaa aaacatctcg tttttatatt ttaatatttt actcatcaat
    13801 atatcaccat ccattcatta tataggattt gctgacacct acaatttatt ccttatattt
    13861 aatagccatc ttcataatgt tcttacttgt tttattgtaa ttgaataact ctttatttac
    13921 aaccacaata tttactttat tgatgttata atcagtcaag tattgtttat ataacataga
    13981 ttagtgttgt ataaagtgag aatttgtaag caatattaat gtaaaatatt tgaaaaactt
    14041 tatataaaaa tatacaatgt tacttatgga attaaactat aacaaattaa agcataattt
    14101 aaggagacaa aattataatc tgtctcctta aatattaaaa ctatatttta aaacattact
    14161 aaatactata caaatttcaa atatagtata aaattaatta gactttaaca ctattagctc
    14221 catcttcttc ccatttttta atttctttta atctatctat tataggtaaa tgctgagtac
    14281 attcaacttc acattttcca caagatatac attctttagc ttttgcctta ctattcattg
    14341 tgtaattcca atgtcctaag ccataaacta actttgtcat ttcttcatca ctctttttaa
    14401 acatctgctt ttcattataa aaaagcatat aggctggggt attaatacct tttggacata
    14461 ctttacaata accacaacct gtacatattt cgttcatatt cttatttaat ctagtctcaa
    14521 tatcatgtat atcctcatct gaataaattc tattttcgtc tgcaatttta catgcatcat
    14581 ctatatcttg ttttttatta aaccctatca aagatatact tatttgtgaa attcctatgt
    14641 tgaatcttaa agctgattct atagtacttt cgccatcttt tgatagaaaa gaaagttctt
    14701 tattatattt tggaatagtt ccaccactta atggattcat tgccacaaca ccatatccat
    14761 tatcaacagc atatttggca ccttttagcc tatatggaaa gtttaatata tttattccca
    14821 tagttacacc ttcaaactta ttttcactta gaatatctat aacctcatct cctggttgat
    14881 gagatgaaaa tactatatgg tctatcaatc cttcttcttt acatttcaat aatccttcat
    14941 attgacctcc aggtctcatt gctagttcgt aatgttccat ctttctaata ttccaaacat
    15001 gataaaaatt tattttagat acgcctaatc gttctattga tttttttaca gcctctatag
    15061 ctttttctga tgtatcacat gcttttggct tttctttagt tgatacataa aaatcggtct
    15121 taccttcctt tatcatttgt ctaaaggcta gtccaaatat atcttcactt ctgtcatcac
    15181 aataccctgg tgctgtatca aagtaattaa tacctttttc atacgcatat ttaactaaat
    15241 cagcattttc ttgattactt ttttctaaat caaaccttaa tcctccatag ccaactactg
    15301 aaaccatttt ccctgtcttt ccatattcaa cgtatctcat tgtaaattcc tccttagata
    15361 tattaagact taatttcttt atagaaaata ttataccata aataattttt ataaattatt
    15421 tatggtataa tatttttttt ataaattata tttttttata acttgtattt tataactaag
    15481 ttaaaaacta taaaaaggtt caatatggac tattacttga gcattttcat taatttcttc
    15541 ttgtatttgt ttctcaattt tatgtgttaa atcatgagat tgctctacgc ttataaatgg
    15601 gtctaccatt acatgcatgt caatataaat agaacttttg ctacctctac ttctgatatt
    15661 gtgaacatcc cttacctcat taaattcaaa cacaatttct tttatataat cctcatctac
    15721 agccactcta tcgaccaaaa ttccaataga tggcctaaat attccataag ccgaataaat
    15781 tatgaatgca gaaataataa taggaactat agattctatt attactggaa atcctaactt
    15841 aacaccaact aaagtaacca atacacctag agacacaaat atatcacttc ttgtatgcaa
    15901 tgaatccgaa atcaaaacat aactatttag tttagtccca attctatatt catacatgca
    15961 tacaaaaata tttataaaaa gtgtaataat taaagcaatt aaactttcta tagttatagc
    16021 aggtacaact ggattagcta ttcttaacac tgcactaaga attatcttaa ttgctataac
    16081 aaacaacata ccacttataa ataaactggt tataatttca aactttttat gtccataagg
    16141 atgattttta tcttttggtt tagatgcaaa gaaaatacct ataagcccca ctatattaga
    16201 tgctccatct gacaaagaat gaaatccgtc tgctgtcata ctatagcttt ttatttgatt
    16261 accaataatg atttttaata atgctactgc aaaattagca aataaaatta tccacaatac
    16321 ttgcttcact ttcttatagt tatccataac aaattccccc tcccttcgat tcaataaaga
    16381 aagctatcta tcaatatcaa tcaaccagac aataaaatcg tatataagca catttactat
    16441 atttatattc aaaattggtt gtacttgttc attctatttg aaacttaatt ataaataaaa
    16501 atcgtaggta tggttttggt atacataaaa ataagagact gtctcaaaat aaagataatc
    16561 ttctttttta ttgttgaaat gataaaatat atttgatttc atataaaaaa gagtgcctca
    16621 tgaactaaaa agttcatttt gagacactcc cttaaatttt tgatataaac tattaatttt
    16681 ttatactaag ccttctttaa ctaagaatga atgagcaact tcttctggtg atttaccaag
    16741 ttcatccact tgatagttaa gttctctcat agtttcttca tttatttttc ctgaaagcat
    16801 attcataact ttttctaact caggatattt cttcaaagta tcttctctta ctaatggtgc
    16861 tgcataatat ggtacaaaga aatttttatc atcctctaat atcttaagat taaacttttt
    16921 aataagacca tcagttaaaa atgcatctat aacctgagat tcattattat ctaaagctgt
    16981 atatcttaaa cttccattca ttgcttttac atctttgaat tttaaaccat aatctctaga
    17041 aagccctaca agaccatctt ctctattttc aaactccaaa gttggtgaga atgtaaattc
    17101 tttaccatat ttagtcaaat cagaaatagt atttattcca tacttttcag cagtttcagg
    17161 catcatagct aatgtgtaag tattattaaa tccaagaggg tcaagtaaag ttaactgatt
    17221 ttctttttcc atagtatctt ttacaacatt atatgcttca tcagcatctt taatcggagc
    17281 atgtttcatt acatttacca aaagtgtacc tgtatagtca acatacatgt ctaactcacc
    17341 agatttaatt gcattaaacg caacagatga accacctaga tttaaattct tcttaacttt
    17401 aagattcgta tttttttcta ctaattcagc ataaacatta ccaagtataa gttgttctgt
    17461 ataatcttta gaaccaatag ttattgtatc ttttttactt gaaaaactag aactaaaaac
    17521 aacgaacaac attgaaacaa caaccactac agatattact ttaagtgcta tataattctt
    17581 cttaggtgct tttggattta atccttttgg agtaactgca acttcaattt tactaaataa
    17641 ataatcaact aacagtgcaa ttatacatga tgggattgcc cctgctaaaa tcatattatt
    17701 atttacagtt tgaacccctg aaaatactaa atatcctaaa ccaccagcac ctataaatgc
    17761 tgcaagagtc atcaaaccaa cagctgtaac tgcagatatt ctaactccac ccattattat
    17821 aggaagtgct aaagggaatt gtattttaaa taatgtctga tttcttgtaa gaccaatacc
    17881 tttagctgat tctaatacta ctgggtcaat acttgttata cctatatacg tattttttac
    17941 aattggtagt aatgaataaa ccactaccat aaatatggct ggtttacttc ctataccaag
    18001 tataggtaca agaagaccca aaagtgccat agacggaact gcttggacta agtttacaaa
    18061 tccaagtaca aactttctaa gagatgagat tcttgaaata attattccaa gaggaacacc
    18121 aactattata gctattaaaa tagatgtaac agttagactc atatgttgta ttaaaagctc
    18181 tataatctta tctttttgta atagtataaa gttaaaaaaa ttagccaaga acactcacct
    18241 ccatttctaa gaattgttca cttaaaacag aaagtaaact acttctagta ataagcccta
    18301 ccagtttatt ttcatcagaa ataaccggaa tatatccaac cgaattacga ttcattacat
    18361 ttagtatttc tactaaatta tcaccttcat ttacatgcaa aggctcactt gacataatat
    18421 cagcaagtaa tattgactta tcatcaatat ctttcatatc ttttacagta acaatacctt
    18481 ttaatgtttt tgctctatct ataattagta agctatcaac cttactcgtt ctcataattt
    18541 ctattccttg agttacagtt ctagtagaat taacagcaat aggatttttt atcataatat
    18601 ctcttgcttt tatatattct ggattatccc aaactctatc ttctccgata aaatctttta
    18661 caaagtcatt tgcaggcttt ctaagtatat tttcaggagt atcatattgt gctattcttc
    18721 catccttcat aatacaaatt ttatcagcta tctttaaagc ctcatccata tcatgagtta
    18781 caaatattat agtttttttc aattcatctt gaagagaaaa taattgttct tgtaaagatg
    18841 ttctcgttat tgggtctaaa gcactaaaag gctcatccat caatattatt tcagcatcag
    18901 tagcaatggc tcttgcaaca ccgattcttt gttgctgtcc accactaagt tctgaaggat
    18961 acttattcaa aaattcatca ctatcaagtc ctaccatctc aagtagttgc aacgttcttt
    19021 tttcaatttc ttcaacggat ttttcctttt ttaggcgtgg aattagctct atattttctt
    19081 ttattgtaag atgagggaat aggcctgtgt tctgtataac atatcctatt tttcttctaa
    19141 gctctatttc attttcctga gaaattggtt tgccattaat atatatctca ccagatgtag
    19201 gttttattaa tttgtttata agcttcaaag tagtagtctt tccacaacca cttgaaccaa
    19261 tcaaaactac caatgtacct gtttctacag ctagagaaat atcatctaat ataacattat
    19321 ttcctatttt ttttgttaca tttcttatct ctatcataat ttatcatccc ctttttcgaa
    19381 caagcaacct tcttcaatat tataagttgt ttttataatt atatcatact attttattta
    19441 tgtaaacaaa gtacgatttt taggtcacta tttgtctgaa tattgctaca aatataaagt
    19501 atttatatat agtatataat atataatgtc aaaaaacaaa aaatataaaa atattcataa
    19561 aataacacta gcctcaatta tatagtatta aagctagtgt tatttcattt aaataaaact
    19621 cacagttgga tactatccat gtattcaaat aatgtaatat ctttataagt ttctttttta
    19681 tcacttaagt ttgatattgt aactcccaat agcctaattt gttttccatt aacctcaaca
    19741 tttctcaaca agttatctac accacttctt atatcattat attcgtcaat atagtgttct
    19801 aaactaaggc ttctagttat ctttgtaaag tcctcatatt taatttttaa tgttaatgtt
    19861 tttccaaact tctcagagtt ttttaatcta tgacatactt cttcacaaag ttcatcaaga
    19921 atgttgcgtg tttcttcttc atcaatatcc aaattatgac ttaatgtagt ctcagcccca
    19981 acagattttc taaccctatt tggttccact ggtctataat ctattcctct agcaaattta
    20041 tataattcat aacctctttt tttgaatata ttttcaagtt caaataaatt taaacacctt
    20101 aaatcatatc cagtttttat acctaaattt ttaagagtat ttgaagtaac ttttccaaca
    20161 ccaaaaaatt tattcacagg taatttatct aaaaagtcct gggcattttc ttcggttatt
    20221 acagttaacc catttggctt tcttaagtct gatgcaatct tagcaagaaa cttattgtat
    20281 gacacaccag ctgaagaagt aagaccaact tctctaaata tatctttttt tatttgcttt
    20341 gctatctcaa ttgatgaatc aatatttttt ttattttttg ttacatctaa gtaagcttca
    20401 tctaaagata atggttcaac taaatccgtg tatctataaa atatatctct tatttgctct
    20461 gacacactct tataaacatc aaatcgaggt cttacaaaaa ctgcatatgg acacctattg
    20521 taagctacag ctgatggcat agctgaatgg ataccaaatt ttctagcttc ataagaacat
    20581 gttgcaacca cacctcggct ttgtggattt ccaccgacaa taacaggtct tccttttaat
    20641 tttttattat ctctttgttc tatagatgca taaaaagcgt ccatatctat atgaattatt
    20701 tttctataat gtttcattat tatcaccttt taaagtataa atagattaat tttatttata
    20761 ttacttttta attataccaa acttatgttt ctttttggta ttttttcatt attttcttta
    20821 tatattcata ttttccataa ataaaaccat ctataatcca ttaacttcaa tataatttat
    20881 aaaaaatcct taaaaacact tgcaaaaact atataaaagc tttacaatag gtattccaag
    20941 gaactaaatt taaaattcct tatggataac agtatatata tttaatttat gggatgtttt
    21001 aaaaacaaaa gactgtctta tacaagacag cctactgatt ttattataat tgtaatggac
    21061 tgctcaaact attactttaa aacagcccat atttttttat ataattttaa ctatagtaaa
    21121 gattttgtga aggatattct gggtcacaag ttacatctat accaagcttt ctaaatgttt
    21181 gttcatcatc tctatttaat atagtagtag aatgagcttg acaccctttt aataatgcta
    21241 acttttccat agcaaattgt gcagttggat tagttacagc acaaatacta agtgcagtta
    21301 acacctcttg gcaactaagt gaaacatttc tactagctaa agtctttgtt tttaagttta
    21361 tgataggttc taatattaca ggtgatatta agtgcatatc atcagatata ttggctaaat
    21421 gtttaatggc atttaatact actgctgcag ttccattcat taaatcagaa cccttaccag
    21481 ttaatattgt accatcttct aattctaatg caactacagg acaagtatca ttcttgtcag
    21541 cactttcttt caatttatta ctgtagtttc tagcaggtaa tacaacattt ctatcttctg
    21601 gctttaagtt aagctcttcc ataatcattt tcattctatc ataagcacct ttatcaactt
    21661 gtcccttttt atactcacag gcagttttaa agtatcttct tattatttct tctatagaag
    21721 cttttcttat tacttcatca tctattattc caaagccaac tctattaaca cccatatcag
    21781 ttggagattg gaaaattgat tcttttccag ttatcttttc tattattttt ttaagcactg
    21841 gaaataattc caaatctcta ttgtagttta ctgacatttc accatacgtt tcaaggtgga
    21901 atgaatctat catattcaca tcttttaaat caactgtagc tgcttcataa gctatattca
    21961 aaggatgttt tacaggtaca ttccaaactg ggaatgtctc aaacttagaa tatccaacta
    22021 cattacctct tttattttca tgatacaact gacttagaca agtaccaagt tttccactat
    22081 taggtcctgg tgctgtgact actactattg gctttgtagt ttctatatat tcatttgctc
    22141 catatccctc atcactaact atagtctcaa catctgacgg atatcccttt gttggataat
    22201 gcttataaac tttaattcct ctacgttcta atttatttat aaatacagtt gtagcaggtt
    22261 gtccttcaaa tctagtaatt actacactat ttacatctag ttcataacct cttaaatcat
    22321 ctattaatct taaaacttcc atatcataag tgataccaaa gtctcctctt attttgtttc
    22381 tttctatatc tcctgcatag acacaaatta caacttcaac tttttctttc atatgttgta
    22441 atacttttat ctttgcattt tcatcaaatc ctggaagtac tctttttgca tgaaggtctc
    22501 ccatcaattt tcctccaaac tctaaataaa gtttgtcaaa attatttact ctttctaaga
    22561 tatatttaga ctgctcttct aaatattttt tgtggtcaaa tcctactttc atcttaacac
    22621 ctcactaaat taaatttctt gttgataaca gtatatttat atatattttt ttagtaaaac
    22681 atcttataaa aaaatgttaa tactttttca ctcaaagtaa tattatacaa ttaagattaa
    22741 tttttttcaa gatagtttta tggtaaataa gatataccta tataactaat aaaataagaa
    22801 tattatattt ttatatataa gtataatatt ctcaccttag cattaattta tttcaatatt
    22861 ttcatatatc ttttattcta aattcaatac ttttctaata tcttctatac cttgataaac
    22921 ttcttgttta gaaaaattca tagcttctaa ttcttcatca gttttttcca taagcatagt
    22981 ataaaattct attgctatat actgcatatt ttcactattt acactcttag ctttatcaaa
    23041 taaaatttct tctgcttcgt ttacatttcc ctgaaaaact aatcttttta aagttataag
    23101 tataacttct tcttctgtat tatacactaa atctttttct tcatattcac tgtatttttt
    23161 tcccaataaa agtcttgcaa ccataagttt tatctcattg aaaatcttat ccactatagc
    23221 catttttaca tctcctataa ataaatttta ttttcttttt aattatatca gactttatcg
    23281 taaaaattat tatatgtaaa tgaaataatt caaaaataaa ttttaacaat aaaaaaaaga
    23341 aggtagcggg ggatttccga ctaccttcaa acactagatt gtgattggtt tggtatttta
    23401 tttataccca ccataagctt tattaaatca tctaaattga tttttttagc atccacatcc
    23461 tccatcaaaa acgtcaccac attcacaaag tattaataat aatattaaga aaggtatcca
    23521 tgcacataag tccatttcag cccaacaatc ttggaatgct aagaataaga agaataatat
    23581 tattatccac caagcacatc caccaaatcc gccttcacca aaaaatctat ccaacataat
    23641 atgttacctc ctagtcattt tgtatgtgga gtttcagctc ctaaaatata ctatgtttaa
    23701 ggggtaatat ttgctaatga tttttttaaa tttttttaga tattttaaaa aaaattattg
    23761 attcacactc aatgtcttac catcactatt gataactacc tctccattag aagtcaaaaa
    23821 tgtttttata ttaaatcttt ccaatatttt taaaacacct tcctctggtt cttttttctc
    23881 tgaacatgta ataacagcat attggggtga aatagactct aaaaatgttt cagttaattt
    23941 gtcatatctt ccatgatgag gcattttaac aaaatcatat tttaaagtat ttccatttat
    24001 aaactctgac aatctttctt cctctgcatc tcctgcaaat aaaaacttat gtgctccata
    24061 gcttatatta gtgattattg aataatcatt tgaactctcg tacttgcttt ttgaagccgg
    24121 atgtatgttt atttctgcac tattgattac aatattcata ttatctttta gtagcactgg
    24181 ttctatatca gcttttttaa gtgagtcaat atattcatca tattgcttac tatctttctt
    24241 ataatttgct tgtataatat tctttacttt aacactagat aacacactat ctactccacc
    24301 tatatggtcc ttatctaaat gagttaaaat taaatagtca agtgtattta ttcctttttc
    24361 ttttaaccta tccactattt gttttccatt tttatcttct cctgtatcaa tcattatgac
    24421 tttgttacct atctcaacca atatgcaatc agactttcca gtacttaata tagtagtctt
    24481 aaatgcagta ttttttgtgc tactctgaat attattttga ctcacatcat ttttgtcact
    24541 ttttaaataa gattcattcg ttgattttat cttactatta gtagttatat ttgttacaag
    24601 caataatatt actattatag ataatgtagt aactaaaaac aggatatctt ttaatactat
    24661 tttcctcatc caaaagctcc tataaatttt atttattaca tatttatagt atagcaacct
    24721 taactctgta aaatacacat ataaaaaggg caaattttag gcatatttgt ggcatgataa
    24781 aaaaatgact gtaccaaaac aattatcttt ctaaatttac atcttcaaat tctttaatct
    24841 tatcattaga taaaacactc tttttagtag attgtccatt ttttctatca aaagtacttt
    24901 tagcttttac acctgataaa aacttacctg ctccattaat acaaccttct tcacacatca
    24961 ttccttctat aaaattacct tctaatcttc ccaatttagc cattgtcatt gtctttttaa
    25021 tttgtcttgg accagatact tttataggtt caaaatcaaa actaagcccc ttttcagcaa
    25081 tatagttctg tactgatttg gcaagtccac cagatgctcc aaatcccctt ccaaaaatcg
    25141 aaccatcatt tatatcttca tttgaacatt ttgataaatc tatattaaat gcttcaaata
    25201 atgccattag ttcttcaaaa gtaagtacat aatctactga atcttttaaa cttttctctg
    25261 ttgcctcact ctttttagct gtacatggac ctataaatac aactttagca tcagaatcta
    25321 ttttttttac taatcttcct gttgctacca taggagacac tgtagatgat attctatctt
    25381 tttcatctgg gaacatcttc tctatatact taaaaaatcc cggacaacat gaatttgtca
    25441 tgtaattatc tcctgatttt attctttcaa taagttcatt gctttcatga actgtaactg
    25501 catctgctcc acaagaagct tctatcatat ctacaaatcc cagtgatttt atagcatttt
    25561 ttaactgacc atatgtaata ttactatcta tttgacctgt tatagctgga gctactattg
    25621 catacatatt ttctttcttt gcaagttttt ttgcaacagg tgctatcaaa ctcttatcag
    25681 atattgctcc aaaaggacat gctgacatac atgctccaca atttatacaa ttatcttcat
    25741 gtatcattgc tttcatagtg tttctattaa aatccaaagc ccctgttgga caaacgcttt
    25801 tacaaggtct tacaacttct gatatagcat catattgaca tgaacttttg cacattccac
    25861 aagccttaca taaatcatga tttatatatg ctctcccatt tatataagaa attgccccaa
    25921 atttacatgc ttccttacat ctatgagcca aacaccctct acaaagctct gtaactgtaa
    25981 attcatttat tggacatcta tcacatgctg cttcaactat atatataatt tgctcatctg
    26041 tgtccaaact aactgaatct ggatttatat ctccatattt actatttgga gttaaactta
    26101 atgcaagttg cgccctttca agaactactt ctctctcttt ataaacacaa catctatatc
    26161 tcgccttttc tcctttaatt atttcatatg gaattttttc tatattttct tttgttaaat
    26221 tttcttcaat agatagtctt gctactgctg ttaaaacttc atgttttaac ctttttagtt
    26281 gcgtttcaaa ttcaaacata agtaatcctc cctataaatc acaccttttt gctatatttc
    26341 tactattatg tatattgtat tttttattta tttctataaa ttattataac ataggcatat
    26401 actttgttaa gttatataca aaatattata tatgtaattt ttattttaag tgtatataat
    26461 ttttatttta gtcgttcaat aattaaatat ataaataaaa atagagttgt ccaaaagtta
    26521 aacctcaact tagaacaact ctattaacaa tatgtatttt tgttgctttg tattatcttt
    26581 atttttctaa atttatatta ctaaaatctt ttatctttgt atttgctaag acacttttct
    26641 tagttgattg agaatttttt ctatcaaaag tggctttaac ctttgcacct gataagaatt
    26701 taccagaacc atttatacaa ccaccttcac acatcattcc ttctataaaa ttaccttcta
    26761 gctttcctaa attagccata gtcatagttt tcttgatttc tcttgaacca gaaatcttta
    26821 caggttcaaa attaacacca ataccttttt cagcaagata attttctata gcttttgtaa
    26881 gaccaccagc agctccaaaa cctcttccaa aaattgatgc atcatctata tcttcatttg
    26941 aacacttagc caagtctata tcaaatgcct caaataatgc cattatttct tcaaaagtaa
    27001 gtacataatc aacagctcct tttaattctt tcttagctgc ctcactcttt ttggctgtac
    27061 atggtcctat gaatacaact ttagcatctg gttcatgact ttttataaat ctaccactag
    27121 ctatcatagg agacactgta gaagatattt ttcctacatg atttgggtat gttttttcta
    27181 tataaccaac aaatcctgga caacaagaat ttgtcataaa agcttctccc ttttccattc
    27241 tttcagcaaa ttctaagctt tcatgaactg taactgcatc tgctccacaa gctgcttcta
    27301 atacatcaac aaatcctagt gattttatag cattttttat ttgaccatat gtaacatttg
    27361 cttcaacttg acctgttata gctggagcta ccaccgcata cattttctct ttttgaacta
    27421 actttctagc tacaggagct atcaaactct tatcagatat tgctccaaat ggacatgctg
    27481 acatacatgc tccacagttt aaacatttat cctcatgtat catagctttc atattttctc
    27541 tatcaaatcc taaggcatta gttggacaaa cactcttaca tggtctaaca acctctgaga
    27601 tggcatcata ttgacaagct tttttacaca ttccacaagc cttacataat tcatgattta
    27661 tataggccat tcctccgaca tgtgttatag ctccaaattt acaagcttct ttacatctat
    27721 gtgctagaca tcctctacaa atatctgtta ctgtaaattc atttattgga caactgtcac
    27781 atgcagcttc caaaatatat ataatttgtt catcttcttc tatatccatt gtatctaaat
    27841 ctatcttatc ttgacctggt tgtaacccca tacttacatt tactctctca gatattatag
    27901 ctctttcttt ataaacacaa catctgtatg taggtttctc accttttatt atttcatact
    27961 gaatattatc cattgcttct ctggtaaggt tatcttcttt tccaagtcta gctattgcag
    28021 ttaaaacatc atgtcttaat ttgtgtattt gtgtttcaaa ttgaaacatt ttaataccac
    28081 ccccgtaaat gattttatac gataccatta
//
LOCUS       DAELJO010000030        28036 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELJO010000030 DAELJO010000000
VERSION     DAELJO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.030"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            120..749
                     /locus_tag="KPB88_001162"
     CDS             120..749
                     /locus_tag="KPB88_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053459.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            750..1532
                     /locus_tag="KPB88_001163"
     CDS             750..1532
                     /locus_tag="KPB88_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053460.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1525..2403
                     /locus_tag="KPB88_001164"
     CDS             1525..2403
                     /locus_tag="KPB88_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053461.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2436..3395
                     /locus_tag="KPB88_001165"
     CDS             2436..3395
                     /locus_tag="KPB88_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0053462.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKNDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3709..4401
                     /locus_tag="KPB88_001166"
     CDS             3709..4401
                     /locus_tag="KPB88_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053463.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4411..5415
                     /locus_tag="KPB88_001167"
     CDS             4411..5415
                     /locus_tag="KPB88_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053464.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYHKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5683..6534
                     /locus_tag="KPB88_001168"
     CDS             5683..6534
                     /locus_tag="KPB88_001168"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0053465.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQV
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6638..7384
                     /locus_tag="KPB88_001169"
     CDS             6638..7384
                     /locus_tag="KPB88_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053466.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7384..9300
                     /locus_tag="KPB88_001170"
     CDS             7384..9300
                     /locus_tag="KPB88_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053467.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9579..10496
                     /locus_tag="KPB88_001171"
     CDS             9579..10496
                     /locus_tag="KPB88_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0053468.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYYDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10574..11311
                     /gene="cas6"
                     /locus_tag="KPB88_001172"
     CDS             10574..11311
                     /gene="cas6"
                     /locus_tag="KPB88_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0053469.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11317..13167
                     /gene="cas8a1"
                     /locus_tag="KPB88_001173"
     CDS             11317..13167
                     /gene="cas8a1"
                     /locus_tag="KPB88_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG0053470.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLESLVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFLNVLKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQEYVSSICDVCKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINGLFERNNSLKAQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKIPPEEILQIFIEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            13169..14083
                     /gene="cas7i"
                     /locus_tag="KPB88_001174"
     CDS             13169..14083
                     /gene="cas7i"
                     /locus_tag="KPB88_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG0053471.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEVIGNISELKKLSRADGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14085..14786
                     /gene="cas5b"
                     /locus_tag="KPB88_001175"
     CDS             14085..14786
                     /gene="cas5b"
                     /locus_tag="KPB88_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG0053472.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14797..17118
                     /locus_tag="KPB88_001176"
     CDS             14797..17118
                     /locus_tag="KPB88_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0053473.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEIVECNSNMN
                     IGEYTEKHLVKEFGSLREVQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSNEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIVKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            17133..17684
                     /gene="cas4"
                     /locus_tag="KPB88_001177"
     CDS             17133..17684
                     /gene="cas4"
                     /locus_tag="KPB88_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG0053474.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINTQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            17687..18667
                     /gene="cas1b"
                     /locus_tag="KPB88_001178"
     CDS             17687..18667
                     /gene="cas1b"
                     /locus_tag="KPB88_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG0053475.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPIISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18670..18936
                     /gene="cas2"
                     /locus_tag="KPB88_001179"
     CDS             18670..18936
                     /gene="cas2"
                     /locus_tag="KPB88_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG0053476.1"
                     /translation="MFVIVTYDIVEERSLNRIRKILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19120..20862
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19120..19148
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            20923..21351
                     /locus_tag="KPB88_001180"
     CDS             20923..21351
                     /locus_tag="KPB88_001180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0053477.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(21806..22576)
                     /locus_tag="KPB88_001181"
     CDS             complement(21806..22576)
                     /locus_tag="KPB88_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053478.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLNLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
     gene            22900..23445
                     /locus_tag="KPB88_001182"
     CDS             22900..23445
                     /locus_tag="KPB88_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG0053479.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            23477..24613
                     /locus_tag="KPB88_001183"
     CDS             23477..24613
                     /locus_tag="KPB88_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG0053480.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            24617..26008
                     /locus_tag="KPB88_001184"
     CDS             24617..26008
                     /locus_tag="KPB88_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0053481.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            26084..26959
                     /gene="dpsA"
                     /locus_tag="KPB88_001185"
     CDS             26084..26959
                     /gene="dpsA"
                     /locus_tag="KPB88_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG0053482.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYTLDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            26962..27552
                     /locus_tag="KPB88_001186"
     CDS             26962..27552
                     /locus_tag="KPB88_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG0053483.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(27759..27959)
                     /locus_tag="KPB88_001187"
     CDS             complement(27759..27959)
                     /locus_tag="KPB88_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053484.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT        11259 a         2663 c         4701 g         9413 t
ORIGIN      
        1 ttaaaaaatg tagataaaaa ttgttttatt aagaaaaaat taatatattg acaaaattta
       61 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
      121 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      181 ttattaaagc aaatttgata aaaaaggggt acataataac agaatgtgaa ccacagttag
      241 aatatgtaaa aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt
      301 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat aattttgaag
      361 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      421 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      481 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      541 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      601 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      661 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      721 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      781 ggctagtaaa gagtgttttg atatttttta taattctttt attatggaaa attacaaatt
      841 atcttggtat ttggagtgat tatatattac catcacctga aaaggtatat agtacatttt
      901 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
      961 tgataggttt tgcaataagt actgctatag gtgtgccttt agggatattc tttgggattt
     1021 atagtggagt atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1081 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1141 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1201 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1261 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1321 gatatagctt tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1381 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1441 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1501 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1561 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1621 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1681 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga aattaggttt
     1741 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1801 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1861 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttagcagtaa aattataaat
     1921 gataatttta gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg
     1981 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     2041 tcttatccaa atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt
     2101 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2161 aggattgata tgcaaaatga agttataaga atacataagt caactaataa aggcgtgata
     2221 tttgtaactc atgatattga tgaagcgtta aagataggca agaaaatagt tgttttcaca
     2281 gacgaaagaa aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2341 gattactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt
     2401 taaagaaatt aataatattt taaggagtaa agtaaatgaa attaaaaaag ctattgatag
     2461 gtataacgat attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2521 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2581 taattcagaa acaggatgat ttatttggaa aggaatttaa aaatgataat ataaaagtta
     2641 acttccacga aattactaca ggtccagagc aaacacaagc tttagctgct ggagaaatag
     2701 acttcttaca tgcactaggt ggtacatcag ctttaatagc tgcatctaat ggagtagagt
     2761 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2821 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggccca aaagggacta
     2881 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     2941 aatttgttaa tatgggtatt ccagaggcat cagcagctct ttctgatggc agcgtagatg
     3001 ctgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3061 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3121 agaaacatcc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3181 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3241 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagaca
     3301 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3361 tgaacataaa agaattaatc tataatgcta agtagaaact atgataaaat aatactaagt
     3421 aagaaatata ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3481 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3541 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3601 aattgtgaag ttagtaatat gattaaattt atatattgta aaaagattat aatatattaa
     3661 aattggactg ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa
     3721 gtatatattg tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa
     3781 tatggatttg aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt
     3841 tttaagccac acttagttct attagatgta aatttaccta aatttgatgg tttctactgg
     3901 tgtagatgta ttcggcaaca gtcgaattta cctataattt ttatatcagc tagagatggt
     3961 aatgtagacc aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt
     4021 tactatgatg tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat
     4081 tcacctaaaa tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg
     4141 ttagaactag aattaaatgg tgagaatcta attataacta aaaaagaagg tatactgatt
     4201 gaatgtctta tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata
     4261 tgggatgata ttgagtttgt agaggaaaat actttaaatg taaatgttag caggataagg
     4321 aaaagatttg aggaattagg tatagataat gctgtccaga ctgtccgagg gttaggatat
     4381 aaattaaata agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt
     4441 tatattttta tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag
     4501 tttattagtt ttggagagag tttatatatt cttttattta gttcttttat attgttatgt
     4561 tttttaatat ataagtatca taaaaataga gaagtatata atctgtttga aaaaggagta
     4621 cattctttgg aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca
     4681 gttattttaa aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata
     4741 catagtgaac atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca
     4801 gttataagcc ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa
     4861 gaaatagata gattaaataa agggttagat atggctatgc attttgcaag ggcagataat
     4921 tttcagaaag actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta
     4981 aatgaagaaa aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaag
     5041 tttacttatg tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca
     5101 aatggagtga aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat
     5161 aataaatatg taattttaag tataatagat gaaggaattg gaattgtttc taaagattta
     5221 aaaagggtat ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca
     5281 ggtatgggtc tttatctagt aaaaaaggta tgtgatgatt taggccataa agtaagtata
     5341 aaatcagaag taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt
     5401 ttaataaagg tgtaaatagt ttgataaaga tgtaaattat ctgttacaat agggttttta
     5461 gttaattgta cttttttatt atacaattta ttaaatgaaa tattcatagt taaataatca
     5521 aaatttaaga gagtttattt gcaaacctta catagatgta aggtttgtgt aatgtttgca
     5581 gatagttaaa ttttctaatc taaattacaa ttaatataag aaataacata agagaaataa
     5641 cataaaaaaa gaaattttgt aaaaaaggaa ggatgtttat tgatgagttt agatgttatt
     5701 tttatattga aatcagtcat aatagctata gtagaggggc ttacagaatt tattccagtt
     5761 tcatctacag gtcatatgat tttggttggt aatttaatag attttaaggg gcaatttgct
     5821 gaaatgtttg aggttgtaat acaacttggg gctatattag cagtagtagt actttattgg
     5881 aaaaagataa aagatagcgt tatagaattt tttaaattta tatttactgg tggaaaagaa
     5941 ggaaagattg gttttagatt tggaatgaat gtaattatag gttgtatacc atttgcgatt
     6001 ataggagttc tattttacga taatataaaa tcattgttta atttacaatc tgtaattata
     6061 ggatttatag tgggtgggat tttattatta gtagtagaaa ctttgtttag aaaaaagaat
     6121 cattccacag ataatataga taaaattact ccaatacaag ttttaaaggt tgggacatta
     6181 caagttttgt ctgcatggcc aggtatgtca aggagtgcat ctactattat gggtggatgg
     6241 atagcaggac ttaattctcc tacagcagct gagttttctt tctttttagc agtaccagct
     6301 atggtggctt cttcaggaaa agatttattt gaatttgatt attcaataat gacaccaaca
     6361 ttgtggattg ctttggtagt tgggtttata gtagctttta ttgtatcaat aatagttatg
     6421 gaaaaattcg tgaatttttt aaagaaaaaa ccaatgagag tgtttgctgt atacagaata
     6481 ataatgggtg tagtacttgc agtattagct tttactaaca taattagtgt atagaaaaaa
     6541 tagatttatt atctataaaa attaaactaa gataaattat aattaacaat tatatatatg
     6601 ttatttcaaa aattaaatta ggaaggatag ttaaataatg aaaattttaa ctgtaaataa
     6661 tttaagtaaa gtatatggta aaaaaataat tttcaacgca ttaaatgaca taaattttag
     6721 tatagaagat ggagaatttg taggaattat gggtccatct ggtagtggaa aaactactct
     6781 tttaaatatg atttctacta ttgacaagcc aactacagga actatggaat taaagggcaa
     6841 gaatccttta ttgttaagag gtgaggaact tgcattactt agaagaagag agttaggttt
     6901 tgtatttcag gattttaatt tattggatac attaactata ggagaaaata tagtattgcc
     6961 attgacatta gataaggtca gtgtaaaaga gcaagatgag agattgaatg aagtatctac
     7021 aatattaggt ataaaagatt tattaggaaa aagaactttt gaagtatcag gtggtcaagc
     7081 tcagaggact gctattgcta gagcattgat taacaatcca tcaatacttt tagcagatga
     7141 accaacaggt aatttagatt caaagtcatc aaaggttgta atggagctat ttcaaaagat
     7201 aaataaagaa aataaagtta ctactatgat ggtaactcat gacccattag cagcgagcta
     7261 ctgtagtaga atcttattca taaaagatgg gtctatatat aatgaaatat ataagggtag
     7321 tagtagagag cagttttatc aagagataat ggatgtactt acattattgg gaggcgataa
     7381 ctaatgaata taaaacagtt tgctattaac aatatttcaa gaaatattaa agcgtattta
     7441 ggatacttag caagtattgt tatatcatct tcactgctat tttcttttat tatgtttaca
     7501 agtcatccag atttagatgt atctcaattt cctgattatt taaaagaagg tctcaagatg
     7561 agtaagataa ttgcatatct attcttattc ttctttgtgt tttattcagt tagtgtgttt
     7621 cttaaaagta gatataaaga atttggaata ctttatatta caggtatatc aaagcgccaa
     7681 gtaatgaaat taatctttat agaaaatatg ataataaata tagtatcttc tgtaattgga
     7741 ataataatag gcttaatctt tgcaaagata tttttagtgg ttatgtctac ttttttagaa
     7801 ttatcaccat taaaattcta tataccattt aatgcgatat tgtctacttt aatctatttt
     7861 atgattttag ctgtattaac atcaatattt acttctttta tagttaaaga aaatcaggtg
     7921 ttgaagttac ttaaaggtac aaaaactcca aaaccagagc ctaaaacatc actaatgctt
     7981 gctatattaa gtatttgttt gtttataata gcgtattata atgctattac ctcaaccgac
     8041 caatatacaa catatctgcg cttagttcca gttacatccc ttactatagt agcaacttac
     8101 ttgttctttt ctcaatttag tgtatttttt atgaagaagc taaaattaaa caagagattt
     8161 tatagaaaaa atacaaatat gctttggatt tcgaacttga tttataaggt aaaggataat
     8221 gctagaatat ttttcttgat aactataact tctgcagtag catttactgc tataggtaca
     8281 gtttattcct tttggaaaga tgtagagcgt caaattaatt taatatatcc taacacaata
     8341 tattattcaa caatgacatt gcataatgac cctaaaaagc ctgatagcaa agaaaaagac
     8401 aaggaaagaa tgatgtttat agaaaataat ctaaaaaaag agaatgtaga ctatacaagg
     8461 actaatggtg aatttaaaac tgtatttccc aagaaaagtg attttaatgt cagaattatg
     8521 aaagaatcta agtacttaga aattacagaa actatagggg ttaagcctat aagttttgaa
     8581 gataatgaat gtatatcatt attatcatca agtttaccag gaaataataa cgtcaaagaa
     8641 aatattattg tagggaatag aggtttaaag gtggcaaaac aagtggaaga atgtgttatg
     8701 ccagcatatt acaaaaatat gtatgtggta aaggataatt tttatgatag tatagaatca
     8761 aaatttatag tggatagatt tactgcattt gaagtagagg attcaagtaa aactatagag
     8821 atatgcagag aatttgaaaa taaatttaat aatgagtcag ggatgcaacc atatttgttt
     8881 ttctctaaag aattaatgat agaaactggt aaattaatgt attctacatt tatattttta
     8941 gcaatattta taggtttaat tttctttgtt actacaagta gttttttata taataaattt
     9001 tatatggatt gccaagttga taagaaaaag tatgaacagt taaataaatt ggggatgaca
     9061 tataatgaga ttaaaaaagc ttcaacaatt gagattggaa tagtattttt gtttccttat
     9121 gtagtggctg ttatacattc aatatttgca cttactgcac ttaaaaattc ttttgatata
     9181 gaagtagcat catcagctgt tttggtaatg ggaagcttgt tcttggttca aatagtttat
     9241 tttttaattg taagaaataa ttatttaaaa gagataaggt taaatttagt caatttttag
     9301 ttactattat taaatgataa taaaaagggt ttaaaattaa ttaatattac tggaattata
     9361 gatgtttatt ttttcagtaa tattttttat tgtattaact attaaaattt tgtacaaata
     9421 taaaatataa tatggtaaat gtcaaaatta taaaaaaatt ctacttatat aaaatataaa
     9481 gaggttatat gtacatatgt gtagaaataa ataataaaaa tatcaattaa tgttattagg
     9541 gggggttact cattagtaaa ttagctaata cacttagaat gatgatatta attcaaagta
     9601 gaggcaaaat gaaatgcaaa gaattatcag aagagttgga agtaagtgaa aggcaaataa
     9661 aaagttataa gatgtattta gagcaagctg gtatatttat aaattcaaca cctgggattt
     9721 atggtggtta tgaaatagat aaatgtaata gcatctcatt aattaaatta ttagatagtg
     9781 aagtttcaat tctagatatg ataaattcac agttagagta taataatgat atttacaaaa
     9841 atgaatttaa taatattgta gaaaaaataa aagcagtatt aaatacagga gaaaaaagcg
     9901 atacatatat ggattatttt acagttcaag cacaacgtaa ttgtgattat gagtctgaaa
     9961 aaaataagtg taatgaaata attagagcat ataccacaaa acacaaattt tggatagaat
    10021 attattcatt aaattcagga aatagtgaaa gaattgttca tccatatggt ttatttaatt
    10081 ataaatcaga tacttatatg gtagcttttt gtgaaaaaag atttaagttt atagacttta
    10141 aattatgtag gattaaagat tataaggttt tagaagaaaa atataatgta gataaaagtt
    10201 ttagttggga tgaatatagt aaaaatagta taggaattta taagggagaa gaaattaatg
    10261 ttgttataaa aattagtcat ccattttcaa caattataaa ggagaaagtt tgggttaata
    10321 atcaacaaat catagaatat tatgataaat ctataatgtt taaagctaaa atgagaggtt
    10381 atgaggagat aaaaagttgg attttaagta tgggagctta tgttgaggtt gtagaaccag
    10441 atagacttag aaatgatata ttgtcagaga ttgaaaaaat gaaaaaaatt tactgatttt
    10501 ttcaaataat ttatacttgg accaatatac ttcacggtct agtgttaata tattaattga
    10561 gagaggtgat attatgaaaa tgaaaattga atttagtaca ggttgtatac caatatctta
    10621 taattcgctc tttatgagta ttataaaaga agcaataaga aaatctaatg aagattacta
    10681 taaaaacatg tactattata aagaaaagaa taataaaaaa actaaaaatt ttacattttc
    10741 ggtttatata aaaaagtata gtattgaagg tgacaatttt attatagaag ataaagtaat
    10801 attaaatata agtactccag atttagaatt gggttttcat atatacaatg ggttaatgac
    10861 ttctaaaaaa tgtttatata aagactatga acttacaaga attagaatcg atttatcaag
    10921 agaaaagaag gtaacagaag aaagggtact atttaatgca ttgtctccaa tctgtgtaaa
    10981 gtctaaagaa ggaaaattct tggagataac tgatgataga tacatagaag aactaaacta
    11041 tataactaat gaagtggtta aaaattatag ggggaatgga ttaaaaagaa agttagaatt
    11101 tgaaaatata ggtctcaaaa aggtagtagt taaagagagt ttaagagaat ttaaaaaaat
    11161 aacaggtaaa gagtatcaat atattaatgg ttacaaaggt aaatttatat tgaaaggtga
    11221 tattgatgat ttaaatctta tctacaatct aggaattggt tttagacgtg ctcagggatt
    11281 tggagatgta gatattttag aatggaggtg agataaatga agttaagagt ttatagagga
    11341 gattggtttt ttaatatggg tatagttgga tttttaaata taataaagaa agcagataaa
    11401 caagctgaaa tatttattat ggaagattat atcgaatttg atagcttatt tcttgaaaac
    11461 tttcatgagt attattttaa ttactttatg gatgaatatg atgtatcaaa aagaattaaa
    11521 aaaaatattg attacagtat aaattttatt aaatctaagc ctgatagaat caaagatgga
    11581 attaagaaaa ttaaagatag tgtgaaacaa caaaatgata agattaaaaa atttgatgaa
    11641 gaaaaattta acatgataaa agagaagcta gatagcatgt ctaagataaa aagttatgat
    11701 gaatttgaca gtttagaatc tttagtagat gagactatag atatatttaa aataaaatca
    11761 ataaatgaca ggcttacagc aaatctttat aaatatgtag ttcaagataa ttattttgga
    11821 caagttagtt ttcttaatgt tcttaaggcg aaattagatt tagatggatt aaaacaggta
    11881 atgtttaatg attatttaag acaaattata tattttggag agttagaaga tttattagaa
    11941 gagaatgact atgataagtt gaaaaactat ttaaatgata gattaaatag tatagctaag
    12001 gatataaatg aaaaaaaagt tagtaagtct agtataaata ctatagaaaa aattatgaag
    12061 gaaataaata aaaactttat aaagaaaaat aaaagcatag aagatataaa agagtatatg
    12121 gatagtttag aagtatgtga aatgtgtggt ttatataagg gaatattaga tgaatactca
    12181 gaaagtaatt ttgctccact tggagtaagc accaataatg ctaggaatat gttttggaat
    12241 caggagtatg tatcatcaat atgtgatgtt tgtaagctaa tattgttctg tactccagca
    12301 ggggctactt atacaagaaa gaattattta attaatgaag aaaatgagtt ttatttattt
    12361 gttaacatgg atacttctat aaatgggctt tttgaaagaa ataatagttt aaaagctcaa
    12421 aaaagtgagt cttcagattc caaagatgaa aatcctttta atcagcttat aaaaagtata
    12481 gtagaagaaa atacattaaa aagtgaatgg caacttcgta atatactttt tgtggaattt
    12541 aaagcaagta tagattctaa aaaatgtaaa ataaattatt ttaatattcc aacttattta
    12601 gcaaagtttt ttgtaaaaga aaataaaaaa atacaaagta tatatgatta taaacttaaa
    12661 tcaaatgtgt tagatttaat tttaaaaagt aaagacttaa aacatttaat taataacact
    12721 ttaaggaata tagttaagag taatttagaa agcaataata aatcaaacat atcagggtta
    12781 tcttgtttca atgttgttca attaagaaca atattaaata actataagag aggagtgtat
    12841 aaaatggatt ataaaaagtt gagaaagatt tatgagtcag gttgtgaaat acatgattat
    12901 tatattgata agaatgctaa aaataaaata gaaggaataa catataaaat tttaaattta
    12961 attaaggttg gtaataaaaa tgattttatg caaacaattt taaggatttt ttcaccaagt
    13021 gaaaaaatac caccagaaga aattttacag atatttattg aagaagattt agattttgaa
    13081 tctgtagggt atgcatttgt aacaggatta atttctggaa aatatgaagg aaaagataaa
    13141 ctaccaaacg aaaaggagga aaaataagat gaaaagagga ttaacattta cagtaatagc
    13201 acaggctcaa agtttaaact atggtgaagt gataggcaat atatcagaat taaaaaaatt
    13261 aagtagagct gatggaaata tgtatacatt tgcatctcgc cagtgtttaa gatatgacat
    13321 agtacgatta gcagcagaat tgtttaattg gaatttacag gttgtcgata aagaaaaagg
    13381 tactattcaa tttaaagaca atataagtat aagagaatca gaagaaatgg atttatttgg
    13441 atatatgaaa acaaataaaa aagatgagaa agataaaaaa ggaggttctt tgactagaga
    13501 agctactgta agattaagta atgcgatttc tcttgagcca tatagaagtg atatggactt
    13561 tttaaataat aaaggatttg cagatagaat aggagaacac cctaatcttg ccaacataga
    13621 gcaacattta agttactata catatacggt tactatagat ttatctaaaa taggtaagga
    13681 tggagatata gaacttgata ataaagagaa atgtagaaga gtagtagaat ttttagaaat
    13741 aataaaagta ttgaatagaa atattagagg tagacaagaa aatttatctc ctctatttgt
    13801 agtaggagga gtatatgaaa tagcaaatcc atttttcttg ggtagaatta aattaaaagg
    13861 agataaaaat ggatttaaaa taaataagca agctatagaa gaagtaattc aaggtacttt
    13921 tttaggaaaa gatttaaaag aatttaccta tgttggaatg gtagatgggg tatttataaa
    13981 taaagaagaa tttaagggat tgtttgaaga taatttttta agtgtagata aattttttgg
    14041 acaactagtt aaagaagtaa aggaatatta tggggtgaat taatatgaag gttttgaaat
    14101 tagatttatt tcaagaaact gcatgctaca aaaaaccatt tgctatgaag attacagaga
    14161 catatccact gcctccatat tctacaataa atggattaat tcacaaaata ttaaatgcta
    14221 cagaatatat accatttaat ataagtgtac agggaacata tgaaagtata tttaataatt
    14281 accagacaac atatttttat aaaaaagata gtattacatc tatgccaatg aatagtcata
    14341 tgctattgaa tgtaaatttg ataattcata taggtgggga ttttgattta ttggaagaaa
    14401 tatatgatag tttattaaat tatgacgagc acttatcatt aggtagaaaa gaagatttag
    14461 taagaattaa tgatattaga tttgtagaag tcaataaatt tgaagtagat gaggatatac
    14521 aattagatga ttatgaaaat tataagttat cagaatgtaa tataagaatg cctatatata
    14581 taccaaaaac aagtttacaa gatacagata tagatggtat aagttacaga ctcaataatt
    14641 actatgataa taatgctgtg gaagataaaa aaagggtttg gaataaggta gatagttatt
    14701 atgttgagga tggaaatact atatcaattg gagatatatt attagataat gaaggagatt
    14761 tattatattt taataatata aattaggagg gagattatgt tatatgctaa atctaatcca
    14821 gtggaaacac ttagagaaca tactgatgaa cttttaaaac aaatgaatgt attaagagaa
    14881 agttatggaa aaaatataaa tagcttagat tttctagaag aggagatatt ttggcaatta
    14941 cttgactttg ttatagaatt tcatgatatt ggaaaagcat tttcaccgtt tcaagagtta
    15001 ataaaaagca gaatggatac taaaataaat gaacctaaaa tagtaactca tttagaaaat
    15061 aatgtaggcc ataattatct ttctcctgca tttattgatt atagttatat agacaggaaa
    15121 aaaaataaag aacttagagc agtattaaat caagttattg tatatcatca tgaaagagat
    15181 atctttatag ataaagattt taaaaattta atacaaaaaa tattagatga agatttaata
    15241 aataaagttt atgaactaca gcatgaattt aaagttagat atccaataaa aacagaaaaa
    15301 ttgagtaaag tatatctaca aagtgtagaa aaaagaatag ataaaaatca caagtactat
    15361 aatttatata ttatgttaaa aggaatacta catagattag accacagtgc ttctgcccat
    15421 gagattgtag agtgcaatag taatatgaat ataggcgaat acacagaaaa acatttagta
    15481 aaagagtttg gtagtttaag agaagttcag tcatttgcta aatcgaatag aaacaagaat
    15541 ataatactaa ttgcatctac agggatggga aaaacagaaa ctgctttgat ttggatagat
    15601 aaggataaag cattttttac actaccatta agagtaagta taaatgcgtt atttgatagg
    15661 gctaaaaata taattggagt aggagaagca aatgatacat tcttaggtct gttacattca
    15721 acagcaatag attatttaga agaaagtaat caggaaaatt caaatgagat tgtagattta
    15781 gcaaagttat tatcttgtaa actgacattt tccacaatag accaaatatt taaatttcct
    15841 tttttgtata gaggctatga aaaagtgtac tcaacattag catattcaaa agtagtaata
    15901 gatgagatac aagcatattc accagaaata gcagctgtat tggtaaaagg tatagaaatg
    15961 atacataaga taggtggcag atttatgata atgacagcta ctatgccaac catatatata
    16021 gatgaattaa aaaaaagagg tgtaatgaat agtaacttag ctgacttgac atgtaacaca
    16081 gaaaaaataa gacattgtgt gtctattgta gaaaattcaa tagatgaaaa cttaggtaaa
    16141 ataatacaaa gtggaatgaa taaaaaagtt cttgtaatag taaatacagt taaaagtgct
    16201 gtagaaaaat atgagttaat agaagaaata gttaagccaa aaggaataga tgtcaactta
    16261 aatcttttac attctatgta tatacaagaa gatagagcaa aattagaaaa atatataaaa
    16321 gaatttgcag atagtgatag taatggtatt tggattacta cacaattagt ggaagcatcc
    16381 cttgatattg attttgatga gcttcataca gaaaattcaa cacttgacag tctatttcaa
    16441 cgatttggta gatgttatag aagtcgtgag tatgaagaaa atagcccaaa tatatttata
    16501 tatacagaaa aagctacagg aataggtagt atttatgata aggatattgt agaaaaagga
    16561 cttgagctac tgaaaacttt tataaatgga aaagaatgtg tgaagatgaa agaaaaatat
    16621 aaggtagaaa tggttaagat actatactct aaagaaagtc tagaaggtac agcttttgaa
    16681 aagagattta ccagtgcttt aaatatttta gatactataa cgccatatag tttaggttct
    16741 aaagatgcac aaaatatact tagaaatatt gaaggatata cagtaattcc agaggatata
    16801 tataataaaa ttgaagaaac tttaatcaaa gattatgaag agttgggaca agagcttata
    16861 aatgcatata gtaatgatga tagacaattg ataaataaaa taaagtctaa gagaaaaaaa
    16921 gcaagaagag agatagttaa aaaaactgta aatttaccaa tatataaagc taaacaaaat
    16981 gtaattgaca tacaggtgaa aggtcttgaa gatttaaaaa tacttttata taaatatgat
    17041 atatacgaaa ataagcatac aaaacagata tttggtaaag gtgtgcttat aagtaatgaa
    17101 atagaccaat ttatttgatt tttatattaa caatgtggct aaaatttact aaagaaggtg
    17161 attttgtgcc aatagattta gaagtattaa aagttcaagg tgtaaaaata aattattatt
    17221 atgtatgtaa aagaaaacta tggctatttt ctaaaggaat aaccatggaa caaaatagta
    17281 gtagagtatt gtctgggaag atagtacatg aagattcata taaaagaatg aaaaataaag
    17341 aaatattgat tgatgatatc ttgaagctag atataataaa ggatgagtat gtaaaagaag
    17401 taaaaataag ttcaaagatg aaagaagcag atgaaatgca gttatactat tacttatact
    17461 atttaaagaa tagtatgggt atagagaaaa aaggaactat aaattatgtt aaggaaaaga
    17521 aacaagaaga attgatattg acagaagaaa aagaaagaat tattgaaaac acacttataa
    17581 atataaatga aataaatacg cagttatctc ctcccaaaat tgagaaaaaa cattattgta
    17641 caaaatgtgc ttattatgaa ttttgttttg tgaaggagga gtaacaatgt ataaagacta
    17701 ttatattata agtaatgggg atataaaaag gaaagaaaat acgatttact attacaattt
    17761 agaaggtgag aaaaaaatat taccagtaga acaagttgat gatattcata tatatggaga
    17821 agtagattta aacacaaaac ttataaatta cataagcaaa tatggggtga tgcttcattt
    17881 ctacaattac tatggatatt attcaggtac atattatcca aggaagaaaa atgtatcagg
    17941 attcagttta gtatgtcaat cagcctgcta tttagataaa aatgaaagat tatacttagc
    18001 aaaatccttt gtggaaagtg catcatacca tattttaaga aatatgagat attacaaagt
    18061 tgatgaaagt ttaattgata ctataaagga tgaactaaat gatttgaatg atgtcaaaga
    18121 aatatcagaa cttatgggta aagaaggcag aataagaaaa acatactata aatcatttaa
    18181 tcaaatatta aaagatgatt ttgaatttta taaaagagaa aaaagacctc ccaaagaccc
    18241 agtaaatgct cttatgtctt ttggaaatag cataatgtat acgaatgttt taagtgagat
    18301 atataagaca caacttgacc ctataataag ctttttacat gaaccatcaa caaagagatt
    18361 ttctttaagt ttagatattg cagaaatttt taagccaatt ataattgaca caataatttt
    18421 tagcctgatt aataataaaa gaattaataa gaatgatttt gaatatcaag acaaaatttg
    18481 ttatttaagt gaaaaaggaa agaagaaatt tttgcaagaa tttgaaaata agatgcagac
    18541 aactataaaa catagaaatc taaatagaaa agtttcatat agaatgttta taaggttaga
    18601 gtgttataaa ttaattaaac attttataaa tgatgaaagg tacaaagttt taaaggtgtg
    18661 gtggtaagta tgtttgttat tgttacttat gatattgttg aagaaaggtc gttaaataga
    18721 attagaaaga tacttagaaa atatttgact tggacgcaaa attctgtttt tgaaggcaat
    18781 attactgaag gaaagttaca taaatgtatt tctgaaatag aaaatattat tgataatagc
    18841 gaggattcaa tatatgttta tgagataaaa aatcctaatt caattaaaaa gaaatgttat
    18901 gggattgata agtattctga tgaaatgttt atataggttt gcagtgagcg atatttatgc
    18961 taaaataggt gttagcagtt ggaatataag ggattgaagg tgtatgataa ctgttatcaa
    19021 ttgcactact gctcgctcac tgcaaatttt gatgttttta ttgaattata attgcttgat
    19081 tgaagtgttt tcaatgtatt caaatatacc tattttgggg ttttatatta actaagtggt
    19141 atgtaaattt tcaataactt ctctagtttt attataaata cgaaagtttt atattaacta
    19201 agtggtatgt aaattatctt tatcagaagt taaaactaaa ccagttgctc cgttttatat
    19261 taactaagtg gtatgtaaat tatagaacgt aactatttca gtctctttac tttgcaagtt
    19321 ttatattaac taagtggtat gtaaatattt taaataatga agttgtttta gattttagta
    19381 atttgtttta tattaactaa gtggtatgta aattaccaat aagttacagc agtaaacaga
    19441 ctaactggag ttttatatta actaagtggt atgtaaatat ttgaatatga gattaagcat
    19501 aggaaagtta ataagtttta tattaactaa gtggtatgta aatcgaaaaa aacttctaag
    19561 aaaaaagaaa gcagtactat gttttatatt aactaagtgg tatgtaaata gagttcgctc
    19621 tacctacaac atgcacagat tgagagagtt ttatattaac taagtggtat gtaaattgtc
    19681 tcaaaaaccg tctttttttg tcctataaaa ggcgttttat attaactaag tggtatgtaa
    19741 atcatcatgt aaagaatact tttgtcctgt aaaatcagtt ttatattaac taagtggtat
    19801 gtaaatattt ttatagctgt ttataattgg tatggtgtta atgtgtttta tattaactaa
    19861 gtggtatgta aattatatag agttttataa tgatatttat tctctttatc agttttatat
    19921 taactaagtg gtatgtaaat ccgtaacctt tttattgttg ttgttattgt tattattagt
    19981 tttatattaa ctaagtggta tgtaaatgaa gcaactgcaa gagtaacaat tacgttagcg
    20041 agttgtttta tattaactaa gtggtatgta aatgctattt gttctgctga tattgaaatg
    20101 aatagaactt cgttttatat taactaagtg gtatgtaaat ttggaaaatt cataaaaata
    20161 aatttaattg gagattgagt tttatattaa ctaagtggta tgtaaataaa gcatcagttt
    20221 taactgaagt aagtgcagta gtcgttttat attaactaag tggtatgtaa atttttcttt
    20281 ttgttatcag tcatgagcat caaacacatg ttttatatta actaagtggt atgtaaatga
    20341 ataaaaataa atcaataaaa acaacctccc aacttgtttt atattaacta agtggtatgt
    20401 aaatgaggtt tacaacattg caactaaata tgagcaggca gttttatatt aactaagtgg
    20461 tatgtaaatt aagttcagct tctggtatct ttttcataca ttatatgttt tatattaact
    20521 aagtggtatg taaatggtaa tgctgatgat ttggttggag ttccttcttc tgagttttat
    20581 attaactaag tggtatgtaa atgctacaca aaagaggtag caggtaaaaa tgacttaggt
    20641 tttatattaa ctaagtggta tgtaaatagg ataaagaaaa gactcacaca agacacagtg
    20701 tcaggtttta tatcaactaa gtggtagata agtagaatat tagcaatatc aacgagtatt
    20761 tagaaactta ttttatatta ataaagcagt atataaaatt gtagaatcaa caatagcata
    20821 tactaaaaca tccaccttat attaactaaa tcatatctaa ataaaataca cttacctaca
    20881 aacattataa aatcaataca aaaatgaggt gaaacaaaat ttatgataaa gaaattaaat
    20941 aataaagaca taaataaaat catggaaata tgggaaaaaa gtacaatcaa agcacatgac
    21001 tttataagta aagaatactg gcaaaataac tacaatactg ttaaaaacga atatatacct
    21061 atatcagata catttgtata tgatgatgga gatgaaataa aaggatttat aagcataata
    21121 gataaaagct ttataggagc tttatttata aaacccaaat accaaaatct aggtatcgga
    21181 ggtaaacttt tagattatgc aactaaaaaa tataaaagtc taagcttagc agtatataaa
    21241 gataataaaa aagcagttgt gttttataat aaaaaaggtt ttaatatagt aaaagaacaa
    21301 gtaaatgaag attcaggatt taaagagtac ataatggaat atagtaaata atatgattac
    21361 attctattaa aaggataatt aatttatttg ccatttgatt ttttaagagt aatttttaaa
    21421 atagactata tgaagataaa gatatttatt atagatgtaa gttaatcaga attgatatag
    21481 ctacaaaaga ggaaaataaa attgtatggt gtatttcaga tttaacaaat ttactattta
    21541 aagataataa gtttgatata atattaaaga tactttcacc tataaactat agggtatttg
    21601 aaagaatttt aaataattat ggaatgctaa taaaggtttg ttaaaattta ataattatta
    21661 gattatagaa aattgccatg aaattagttc atcaagaact agtttcatag caattttaat
    21721 tcgtataagt gtatttattt ttacagattt aaaaaactta tacagtatct aataatgtaa
    21781 tcttattgct ttagcctata aatatttaaa tcttaaaatg ttctctttga gataagctta
    21841 tttcagagtc acaatttcca caacagtaag atgttgagac atctttaaat ttttcagtat
    21901 tctcatcaaa gttcattttt ttctttgcaa taattcctac cttagttaaa ttagaaccac
    21961 aattattaca gttttgaatt ttgcataagt tatatagttt atcaagtgta atatcttcta
    22021 ataattcatc acaattacaa catctgatag aagaaacttc actacacatt tcaataggtt
    22081 cagcttcttt agaagaaaat ttatatgtta tgtcatttga tactttataa cctgtatcaa
    22141 atatattaga attacaactt ggacataagt actttggaag atatatatct tttttttcaa
    22201 aagtatcact actaatatta cagtttaatt catctaaaat tggtctttca tctaggttat
    22261 tacctttatt taagcttaga agataattat aaattttttt atcttcattc tgttttgatg
    22321 tatctaacaa atgctccatt atattagtgt attttttgtt ttttgaaggc atgtttaaat
    22381 tcagataatt gttttcaata gcaaagcata ctatattgaa atagttatat ttaactgcaa
    22441 aaaataataa catttcatca aatactttat ctattatttt gtcagaaata cttatccaaa
    22501 aatctgggga ctctaagtaa actttcctac taagttcatc taactcttca tctatattat
    22561 tttcattaac tttcatatca tcacttcctt ttaaatatct tgtgtataaa ctatttgtta
    22621 ctatttttta tataaaataa aatcttatat aaaaatatat catattataa tttacaagta
    22681 tagtctaaat agagctgttt ttaagcaaag ttttataaat gactattaaa atataaagta
    22741 acagtataaa gtttacttat aaattaaatg taaacctaaa tgattataat agttgacaaa
    22801 aaaatgtctt tattttatac taaaacaaag ctttaataga agctttgtat atgtacatat
    22861 aaactaaatg aaataagaca tatgaaggga attataaaaa tgaaaaatac taaagtaaat
    22921 attcaagatt taactaaaat gtcaatatgt gtagcattat tgtgtatatc atcttacata
    22981 tatataccat tgccatttac tccagcagga gtaacagcac aaacaataat gataaattta
    23041 atagctttga ttttaacacc aagacaagca tttagtacag ttggtgtata tataatgata
    23101 ggtttaattg ggattccagt atttggtgga ggtacatctg gtattggaaa gttgttaagc
    23161 ccaacaggtg gtttttattt tgggtttttg tttgcagcac taattattag tttattaaaa
    23221 ggacgaaaca atgatattaa gagatatata ctaataacaa tttttgttgg aatgcctatt
    23281 atatatttta tgggaacagt atttatgtgc tattttaatc aaatgacagt agaagcagca
    23341 ctttttgcag cagtagtacc atttttaatt ggagatacaa taaagtcggt tattgcatct
    23401 tttttaggca ctaaactgaa caatgtactc agaagaaatc tatgataaat ctataatata
    23461 aggaagggtg taatgtatgg aggaagtttt tatattagga ggattgagaa gtcatatagg
    23521 tctaaaaaat ggaatttttc aatttgttca acctgaatta ctaggagcaa gtgttttaaa
    23581 agatttaatt aaaaagtatg aaattgataa aattgatgag attatatgtg gaaatgcagt
    23641 tgggacagga gggaatatag ctagattaat gactttaaca gcaggagtgt caaatgaagt
    23701 acctgctttt actgtggata tgcagtgtgc ttctgctatg atgagtatag acatagcttt
    23761 ttcaaaggtt aaatctggtc agtgtgattt aattattgca ggtggttttg aaagtagttc
    23821 tctacaacca atgcgcacat atcataagaa tgataagcga tataatacaa ataatccaaa
    23881 ttatacagtt gctcaatttt ctcctgatga taatagccaa aatagtatgc tcgaaggtgc
    23941 ggaaagagta gcagagttat atcaaattga gaaagcagac ttggattttt gggtaaagga
    24001 aagtcataaa agagcaaaag aagcaagaga ggaaaaaata cttgaagata ttatatctcc
    24061 tataaataat agtacctatg atgaaggtat acgtgataag atgagtcaaa ggttattaga
    24121 taggatgcca tcaattttag gaaaagaaac tattacaaat gcagcaaatt catgtcttat
    24181 taatgatgga gcttcattta ttataatatg ttctaagaag tatttagaac atgtaaaaaa
    24241 gaagcctaaa gcaaaaatta ttaatacatg cacaatagga acagatgcaa ccttaagtcc
    24301 aacatcagcg atacaagcaa tggataaact tttagagata gaaagtctta attatttaga
    24361 tgtatctgct gttgaattta atgaagcatt tgcagttata gatgtgttgt ttcaaagaaa
    24421 atatccagag ttaatagata gatataatat atttggtgga gctttagcgt atggacatcc
    24481 atatggtgct tcaggtgcaa ttattgcatt acatctttta aaagcattag aaaagactaa
    24541 gggtagatat ggaatttgct ctatagctgc agctggtggt ctaggttcag ctttactgct
    24601 tgaaagggtg tagattatga actattatga acttttaaga ataaaaaaag agaggcataa
    24661 aaataaaaat tttttaatta tagatggaga aaaatatact tatgaaaata tattgagtta
    24721 tagtgaggaa ctaggaaaac aaatttcttt aggtgaaaat atcccaattt taatatattc
    24781 taaaaatata atgtttcagt tgattagttt ttttgctatt aattattcaa aaaatattcc
    24841 tattatttgt cattataatc tttctaaaaa agtattagat gatattttat taaaaaataa
    24901 tatttcaatc attatatctg atgaatctat ggacgttatt gatttatatg atgataacaa
    24961 taatgaagta gcaaaaaaag ttaatttttc tatttttaat cctaaattta atatatatat
    25021 gtatcaatat aagaacctta taaattattt agataaaagt atttgtatgg gagctttatc
    25081 ttcaggttct acaagtgttc caaaggtatt atatagaaca tatgagagtt gggctggatt
    25141 ttttcctata caaaatggta tttttaatat ctctggggaa tcaatcttgt ttgttaatgg
    25201 aacacttagt tttacaggga atctaaattc tataatgtca gtattatatg aaggaggtag
    25261 tattgtaata agttcaggtc ttaattgtaa atcatggata aggataataa aacaatataa
    25321 tgtaactaac atatacctta tacctacaaa acttcaactt ttggttaaac acttaaaaga
    25381 accagtactc aaagttgtaa gtatttttac agggtctcaa ttattatttg aagatactgc
    25441 aagaaatcta aaaaaatata tgccaaagtc tgaaattatt ttatactatg gggcaagtga
    25501 attaaattat attacatatt tatgctatga tgaactaatt gaaaaacctc taagtgtagg
    25561 cagaccattt ccaggaatag atatatatat tagagaagga aaaatttttg tgaatacaga
    25621 atatgcagta tataatgcaa ccaaacctta ttctgtcaat gatattggat attatgataa
    25681 tgatggatac ttaatatttg aaggaagaag tgatgacatt ttaaatattg gaggatttaa
    25741 agttagttct actaagattg aaaatgaagt taaaaaaatt cctcaaattg aagatgcaat
    25801 tgtattgccg tactcagaca ctataagagg aaatcaaatt gtattatttg taataactat
    25861 agacaaaatt acgaagaaag atttgttgat taaaatgaaa gataatctaa tgaaaaatga
    25921 aattccaaag aaaattatct ttttaaattc atttccatat acctcttctg aaaagataga
    25981 tagattagca ttattaaaaa tgttataagt ataatattta gtaattttag tgaaccaatg
    26041 tattgataaa aacatattgt atatagtaaa ggaggcttac aatatgtcaa atcaatacga
    26101 tataactgtt attgggggag acctgcgaca agtgtacatg gtaaaaaaac taattaataa
    26161 agggtattct gtagtcactt atgggatatc aaatgaaatt ataaatggaa tcgttggaaa
    26221 atcaacttca ctagctgaag cactatccaa aagttccatt attttatgtc caattccatt
    26281 tacaaagaat caagttaata ttgaaaatac ggatgattgt cttgatgcta ctattgataa
    26341 cttgctaatt aatttatctc ctaatcaaat attatttgga ggtaacatac caactaaagt
    26401 ttgtaaattt tgcgagcaac actctattca gatttatgat tttatgaaaa tgaatgatgt
    26461 tgctatttta aatgcagttg ctacagctga aggggctata gcagaagcta ttaaaagaag
    26521 tataatcaat atacatcaaa gtaactgttt agtattaggt tatggaagat gtgcacaaat
    26581 acttgcaaaa aaattataca ctttggatgc aaatgtatgt gttggcgcaa gaaataatga
    26641 tgcaagaagt acggctaatg catttggata ttccagtatt tctttaagtc aacttgatga
    26701 aaaactttta caatttgatt atatttttaa caccatacca gagtgtattt taacgaaaga
    26761 aaggttagaa aaagtatcac aagaagtaac tattatagat atagcttctt ctcctggagg
    26821 agtagattat tctgttgtta aagaagcagg gattaatgcg actctttgcc ttgggcttcc
    26881 tggtaaatat tcaccaaaga catctggtga aattcttgtt aacgctatag aaaatattat
    26941 aaatgaaaga agggattaga aatgagactt gaaggactta caattggagt aggatttact
    27001 ggttcgtttt gtacttatga taaaatattt atagagttag agaatttggt taaagaagga
    27061 gctaatgtac acactatatt ttcagatgta tcacaaaata tcgattgtag atttggaaat
    27121 tctgaagagt ttatgaaaaa agcatatgaa cttacaggaa ataaacctat agttacgatt
    27181 gaagaggcag agccatttgg acctaaagga attgctgata ttataataat agctccttgt
    27241 actggaaata ctgctgcaaa attagcaaat ggtattacag attcaccagt attaatggca
    27301 gcaaaaggtc atcttcgtaa tgataaacca ctagtaatat caatatcaac gaatgatgct
    27361 ctaagcttta atttcaaaaa tattggcatt ctccttaatt ctaaaaatat atattttgta
    27421 ccttttggtc aagataattg caaagcaaag cctaattcta tgattgcaca tactgagcta
    27481 ataattccaa ctatagaact tgctctagag aacaaacagc tacagcctgt tataaaaagt
    27541 ccacatcaat agaattggtt cttataatat tatgtattag atacaatact agcaaagagg
    27601 ctgtctcaaa atagagagaa gcctcttttt cttgttgaaa tgatgaaaat atatttaatt
    27661 tcatacaaaa aaatgcctca tggactaaaa agtctatttt gagctaatct ccgtaatttt
    27721 tggtatatta aaattattta cttaatccaa tttttaaatt agaatttata tcgttttgac
    27781 tcttcaaata tcaatctaac tgttgttgat acaaaagaag aggacataat taaaacaaat
    27841 accgatagtc ctctaataaa caaattatct acataataag ttactattcc aatacctcca
    27901 atcgttaaaa ttacacttat aatcaaagaa aaaatgctaa ttgtttttaa ttttttcatg
    27961 cttatcctcc ttcttataaa cttcaaattt atctatataa tattaaatat attttatttc
    28021 atagtatata aaaatc
//
LOCUS       DAELJO010000031        26430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELJO010000031 DAELJO010000000
VERSION     DAELJO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.031"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            19..1374
                     /locus_tag="KPB88_001188"
     CDS             19..1374
                     /locus_tag="KPB88_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0053485.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKKLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLVLSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(1492..2160)
                     /locus_tag="KPB88_001189"
     CDS             complement(1492..2160)
                     /locus_tag="KPB88_001189"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG0053486.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(2227..2655)
                     /locus_tag="KPB88_001190"
     CDS             complement(2227..2655)
                     /locus_tag="KPB88_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0053487.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVVLLILGTFFIVN"
     gene            2762..3355
                     /locus_tag="KPB88_001191"
     CDS             2762..3355
                     /locus_tag="KPB88_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG0053488.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            3474..4409
                     /locus_tag="KPB88_001192"
     CDS             3474..4409
                     /locus_tag="KPB88_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG0053489.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKTVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(4680..5624)
                     /locus_tag="KPB88_001193"
     CDS             complement(4680..5624)
                     /locus_tag="KPB88_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0053490.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIMYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            5942..7372
                     /locus_tag="KPB88_001194"
     CDS             5942..7372
                     /locus_tag="KPB88_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053491.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            7572..8156
                     /locus_tag="KPB88_001195"
     CDS             7572..8156
                     /locus_tag="KPB88_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG0053492.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(8250..9029)
                     /locus_tag="KPB88_001196"
     CDS             complement(8250..9029)
                     /locus_tag="KPB88_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0053493.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(9087..10343)
                     /locus_tag="KPB88_001197"
     CDS             complement(9087..10343)
                     /locus_tag="KPB88_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG0053494.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            10601..11500
                     /locus_tag="KPB88_001198"
     CDS             10601..11500
                     /locus_tag="KPB88_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG0053495.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEKAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            11524..12972
                     /gene="ddlR"
                     /locus_tag="KPB88_001199"
     CDS             11524..12972
                     /gene="ddlR"
                     /locus_tag="KPB88_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG0053496.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            13031..13837
                     /locus_tag="KPB88_001200"
     CDS             13031..13837
                     /locus_tag="KPB88_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0053497.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            13863..15782
                     /locus_tag="KPB88_001201"
     CDS             13863..15782
                     /locus_tag="KPB88_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0053498.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(16024..16371)
                     /locus_tag="KPB88_001202"
     CDS             complement(16024..16371)
                     /locus_tag="KPB88_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG0053499.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(16358..18286)
                     /locus_tag="KPB88_001203"
     CDS             complement(16358..18286)
                     /locus_tag="KPB88_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG0053500.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(18697..20886)
                     /locus_tag="KPB88_001204"
     CDS             complement(18697..20886)
                     /locus_tag="KPB88_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG0053501.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            21217..21948
                     /locus_tag="KPB88_001205"
     CDS             21217..21948
                     /locus_tag="KPB88_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0053502.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            22028..22663
                     /locus_tag="KPB88_001206"
     CDS             22028..22663
                     /locus_tag="KPB88_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0053503.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(22914..23507)
                     /locus_tag="KPB88_001207"
     CDS             complement(22914..23507)
                     /locus_tag="KPB88_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG0053504.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(23578..24441)
                     /locus_tag="KPB88_001208"
     CDS             complement(23578..24441)
                     /locus_tag="KPB88_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG0053505.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            24604..25773
                     /locus_tag="KPB88_001209"
     CDS             24604..25773
                     /locus_tag="KPB88_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053506.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGEIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITFPLHTSTFDIDEDCLITGVILHVKNVLSF"
     gene            25874..26350
                     /locus_tag="KPB88_001210"
     CDS             25874..26350
                     /locus_tag="KPB88_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0053507.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         9607 a         3936 c         3383 g         9504 t
ORIGIN      
        1 tacagggagt gataataaat ggatacaaaa atatctataa acgaaaacat gagtctaggt
       61 aaaagatttt ttaaatacct agcaccatct gtagttgcaa tgtgggtttt ctcactatat
      121 acaatggttg atggaatatt tgtaagtaaa ggagttggag aattagcttt agctgctgtc
      181 aatatatcaa tgccttttat taattttata tttgcagttt ctctattatt ttcaactggt
      241 gcatctacca taattgcaat ctatctgggg aaaaaagata ttaaatctgc taacgaagtc
      301 ttttcattta acttagtatc aataattata ttatcaataa ttatattagc tataactttt
      361 tttaatttag atagattagc actattttta ggagctacag aatctacaat tggtatggtt
      421 aaagattatt taggaataat aatatttttt aacggattct ttatagtgtc ttattcttta
      481 gaagttataa taaaaactga tggctttcca atattagcta ctgttggtgt aataatatct
      541 gctcttacaa acatcatact tgattacttg tttgtaattg aatttggatg gggtgtaaaa
      601 ggtgctggta ttgcaactgg tttgtctcaa gtcttttcta ctattttctt cttaatacat
      661 tttttaagaa aaaactctac tttgaatttt tcaaaattta gaattgattt taagaaactt
      721 agaaaaattg tatttatagg ttttcctgat tcaactactg aattaagttg tggtatagtt
      781 gttttacttt ttaacttaag tcttactaaa tatattggag aaaatgctct tatttattat
      841 agtgtcataa attatataaa tacattagta cttatgacta tgatgggtat aactcaaggt
      901 atgcaaccac ttactagctt ttattatggt gcaggaaata tagataatgt aaaaaaatta
      961 ttaaaaatgg ggataaaagc cacaataata gcatcagttg ctgtatttgc aatttgtatg
     1021 gctttttcag gaccaatagt ctctttattt atacatccag aagaaacaat gctattcaat
     1081 gaaggagttc gtgtatttaa aatattctct atatctttct tattggtagg aattaatgta
     1141 ataatttctg gattctttgt atcagtagaa aaaccatcaa tttctactgt tatatctctt
     1201 ggtagagggc ttgtaatagt tgttttaagt ctaatttcaa tgatactaat atttggtgga
     1261 caaggaatat ggatgactac tattgtttct gagtttatat gtcttgtttt atctttagtt
     1321 tttctaaaaa agaatttttc tacattagac tctaatctaa ataaggttgc ttagatgata
     1381 agagtagcaa tatatgaaaa tatctaaata aaaaatacct attatacaat cttgcaaata
     1441 ttaaaccttt gcaaaaatta ggattatata ataggtattt tatttatgta attattttat
     1501 ttctaaatat ccatagccat cttttattaa agttcctttt acttcatatt ctacaggttt
     1561 tttaaatatt ggaccatcta cgacaaatgt atgatactct ccatgttctc cacatgggtc
     1621 tgcaccagca ttttttatct tctccacaag ttctctagtt aatttttttc ctaaaaattc
     1681 ctctcctaag tgctttaagt ttacttttgt tacaacacta caaaatccac tgtctataaa
     1741 ttcatatact aaatcctctc tactctcttg ccataatggt aaatctactt tcataccaac
     1801 tgcttcacat ctatcagtat cccattttct atgactttct atatctatat ctccatatgc
     1861 acatatcgtt gctcccatgt ttttagcttt tattaaagcc ctctcaaagc tctcttcata
     1921 tccttctacg ccacactcag caactaataa aggtaatcct atgttgctac ttacttgctt
     1981 aagcatatta tattctaaat tatgtgtcca tgactttcca tgttcttcac ttatagttgt
     2041 caagagtgcc acaggcttat atcctttttt caacattcga tttaatgcta atatactatc
     2101 cttaccacca ctaaatgaca ttacaaattt ttcatcagcc aattttaaca cctctttcat
     2161 ctaactttgt tttattaaca taattatacc aaatttttga gttgcatagc aaattatatt
     2221 aaattgttaa ttcactataa agaatgtccc taatattaaa agtacaaccc ctatgtattt
     2281 gtataaatgc attgtttctt gtcccaaaat tacgctatca ataaaaaatc caactaaaac
     2341 ttgggataca agagttaata taagtgtatt tgatactcct aaatttggaa ttgcctttac
     2401 tgtaaaatat attattagac ctccaaatac tcctcctaat aacaacttat aattaaaacc
     2461 atttatagat actagacact ttaagtcacc tgtaactatt atacttgcca aaaaaaatat
     2521 tgctcctaca gttaagctta taaaggtggc cactaatgct gttgtacttt ttcctagctc
     2581 tccatttata aaagcctcaa gagctgtaga accacctgct aataatgcaa atattattgc
     2641 ccataatttt gtcatggaca aacctccttc tatataattc tattaataga agtttatgat
     2701 acaacctatt aattaattac taagtatagt ttaattttta tacactaggc tttatttgga
     2761 tatgaatttc tgttataatt gttctatcga ttttggaggt gattttatgt acacaaattt
     2821 taatatcgat tttaataact ttaataaaaa agaaaatgct attaaattta tgttaatggg
     2881 agtattactc attatattag gattattatg ccttacattt aagacattag gtataaaatt
     2941 aatttcttgg acttttggta ttgcactttt attctttgca tatttaaatt taaaaaatat
     3001 taatgaacta aaaagatatg ctactaaaga agaaattaaa ccctctatta atatccaatg
     3061 ggttctaata attgcatgta tattactttt tgtatttcct caaaaaatac aaagcatatt
     3121 ttcattattt ttaggatttt atttaatctt taatcaacta gtagcacttg taaatagtaa
     3181 gaataatcct tattctaaat ttacaacttg gaatatagtt aagattctat ttggtatatg
     3241 ccttatatta tctccacttt ttttatctag atttatagta tcgataatgt cttttttcat
     3301 aatattattt ggactagttc tatttttctc tggaaataca gcgagaaaat attaattatt
     3361 ttctcgctgt atttttatat tacttaaaat agaatttgtt taagttatct atttgtggta
     3421 atatgttttt tgtacaagct cagcgtacaa ttttattaga aataggtgat tttttggata
     3481 aatttaaata tgcttttgat aataaaagat atcatacttg gaattactat cttagaaata
     3541 cttttggaga aaaagttttt aaggtatcta taaatgcagg atttacttgc ccaaacattg
     3601 atggaagtct tggttatggc ggatgtacct attgtagcaa agaaggctca ggtgattttg
     3661 ctggaaaccc taaagataat cttatcagtc aattttataa tataaaagaa atgatgttaa
     3721 aaaaatggcc tcatgcaaag tatattggct actttcaagc ctatacaaat acgtatgctc
     3781 ctcttgaagt tttaaaagaa aaatatgaga ctatattaga acttgaagat gtcataggtc
     3841 tttcaatatc cacaagacct gactgtttgc cagatgatgt tgtagaatac ttgtctgaac
     3901 taaataaaag aaccaattta tgggttgaac ttggacttca aacaattcat gattctacat
     3961 ctaaaattat aaatagaggt catgattata aaacatttgt tgaaggtgtt gaaaaattaa
     4021 agtctaaaaa tataaaggtt gtcgtacaca ttataaatgg tcttcctggt gaagattata
     4081 atatgatgat ggaaacagca aagacagttg gtaaaatggg tgtagacggt ataaaaatcc
     4141 atctattgca tgttataaaa gatactccta tggaaaagat gttacaaaat ggtatgttaa
     4201 ctcttatgga acaagatgaa tatatcaatc ttgtctgtga ccaattagaa atacttcctg
     4261 aaactatgat aatccataga ctcactggtg atggtaagag ggatgaacta gtaggtccta
     4321 tatggagcct aaaaaaatgg gaaattttaa atcaaattga tgatacttta aaggctagaa
     4381 attcttatca gggctgtaag tttgtttaat acaaaataat aactatagaa atggttatat
     4441 aaatatagtc ctttctatag ttatttagta tttacaaaac aaaatattaa tttgtacatt
     4501 tatacaatat ataataaaaa atagatagtc taaactgtat ctacttatat tttatttgta
     4561 ctgtccactt tattctataa aatttataaa tagttatttt ttaataccaa atataatttg
     4621 gtataatttt tgttggactc taaaatgtat accaataaat cctttttaag tatataattc
     4681 taaatacttt cagttttttc aatctgcttt aacttgttgg acttataaaa tattcttatt
     4741 tcatcataag aaagattaag tgctactact gcagatatta acatcaacat cactccaaat
     4801 aacttcccta aggaaaaact ttctcctaat atagtgcaac caattattac tgaaccaact
     4861 aattctactg atgccacaac acctgcaact gacaactcta cacctttaga tatacctgct
     4921 gcataaaaaa tatatgcaag tgcagctgga acaatgccta acattagcat acaagataaa
     4981 acatctaaat ccttagcata atttaagatt tcccaaggtc ttgaagaagg aatcataaat
     5041 atagctccaa ataagaaact gtatactaat attgttgaac ttgaaaattc ctttaaagca
     5101 tttttactaa tagttggcat aagtgcatat gctattgctg ataatactcc aagtaaaagc
     5161 ccaaaagcgt ttaaaccttg aaaatccaaa cgtccaccag tcactgccat aattgcacca
     5221 ataaaacaaa gaataagtga aaataatttg cttctagtaa tattttcttt ataacataat
     5281 tttgaaaata ttgctaaaaa cagtggtgat gtatatagta aaactgctgc aattgataca
     5341 cccgcaatgt caattgcttt aaaataacat aaattaaaca ttgcctgaca tataatacct
     5401 attattactg aatacataat tccttttttg tttatcttta agatttgtgg tgtccttata
     5461 gatgaatata cgcctaaaac tataaatcca aaaaacaatc ttgtaaatgc tacttgttct
     5521 gatgtcagtc catttcccat taaaaacttt ccaaataatc caagtgtagc ccataataac
     5581 cctgctattg ctataaatac atacccttta cttttttctc ccattttaaa ttcccttcta
     5641 tttttattta gtcaatttac actcttcttt ttaagcaaaa actatgccat cttaaatcaa
     5701 tatatatcga ctctaaaacc ctagtctttc caataatact aataaatttt caattttttt
     5761 atatttattt ttcgcttact ttaagttgat ttagacttat tttaattgta aaatgagtta
     5821 tatataactt atttaaagtt aaaatagact gaaacttata tataaatatc aaattttaag
     5881 tatattaatc aattaaacct attttaatat atcattaaaa atttaggagg aaaatataaa
     5941 aatgaattta gatttggaat tttatcaaaa aatattagaa gcttcccatg atgaaatatg
     6001 tgtttctgat gataaaggaa taataatata ttgtaataaa gcatttgaag aaaattatgg
     6061 tctaaaaaaa gaagatattt taggaaaaaa tgtttctttt ttagaagact ctggatattc
     6121 aacaaaaagc cccataccaa ttgtattaaa aacaaagact aaattttcac tggaacaaga
     6181 tactcaaaca gggaaaaagc ttatcataac tgctacccct atatttgatg aaaatggaca
     6241 cttagaattt actgttgaaa attgtagaga tataactgaa cttaataata taaaaaataa
     6301 acttgaagac acaaaaaagc aagtaaaaaa atataaatct gaagtagaaa cactatacag
     6361 aactgcactt agaattgaag atactgtaat tatggacgga atagttatga gaccaatcat
     6421 aaacacagta aatcatgttt ctaaaacgga tgtaagtgta cttttattgg gtgaatctgg
     6481 aacaggaaaa agctcacttg ctagatatat acaccacaac agcaatagat ctaatggccc
     6541 atttataaca ataaattgtg caactatatc tccacaactt cttgaatctg aactttttgg
     6601 atatacttca ggtgctttta caggtgcaag tactaaaggt aaagttgggc tcgtagaatt
     6661 agctaatgga ggaactcttt ttttagatga aataggagat ataccacaaa atctacaggc
     6721 aaaatttttg caattaatac aggatagaac ttttactcca gttgggagct taaaaaataa
     6781 aaaggtcgat ataagaatta tatctgctac aaatgttgat ttagtttcaa aagtaaaaga
     6841 aaaaaagttt agagaagatt tatactatag attaaatgtt atagaaataa aattgcctcc
     6901 tcttagagaa agaagggata atctagtgga aattataaaa tattatttta ataggtactc
     6961 aagtgacttc aatctaaata aaactatttc taaagaagct atggatgcta ttgcaaatta
     7021 tagattccca ggcaatataa gagaattaca aaatataatt caaaaaatct tgcttacttg
     7081 tacagataat catataacaa tagatgactt accaaacatt cttacaaaaa atatacatat
     7141 taccaataat ggaaataaaa cacatatatc ccaaattaat aaggttatta tatctgactc
     7201 aaaatctact aattataaaa ataaaaattt tgacacttta attaaagagt atgaaaaaaa
     7261 tataattttg gatgcttatg aaaaatttgg tagttcttat aaagtagcca aacaccttga
     7321 aatctcacag tctaaagcca ataggttaat tagaaagtat actaatactt aattttaaat
     7381 ttgataaata aaaacttaac actatttaca tattgtaaat ttggccattt actgttatta
     7441 tgaatatggt tgaaattatt taaataaata ctgttgactt agtttcatat atttaccatt
     7501 aaacttttat cataaatatc atatcataat aatatattta ataagtctaa taaactatac
     7561 gaggtgtaaa tatgttaatg aatacccatt ttattatggc aaaatcaata cttgataata
     7621 tagatgaaaa taaaacattc tttataagcg aaaaaaattt tatatatggc aatataaagc
     7681 ctgatgcttt ttctaaatat aaattaaaaa aacattattt agatgagtca tttaatatga
     7741 ttgtttctaa aataaactat ctatgcagtc ttaatataga tagtctttct aaatactttt
     7801 cagtaagtag acttagtcag gaactaggag tcatatgtca ttttttatgt gattttttct
     7861 gtgtagctca cagtgaacgt tgggaattta agcatagctt aaataaacat gttacttatg
     7921 aaagagaact tggagcattt gcaaaagatg ttgatttatc taaaattaaa tgtggtacta
     7981 taaatagtag ttttgagaag ttttttacta aactttatag tgattacaaa aagaaaactg
     8041 attatatgaa tgatttaaaa ttttcagctt atacttgtaa tagtgtcata aattatatac
     8101 ttgactctat tttatctaat acaataaaat catatcatat agcaaattgc ggttaactta
     8161 agaattttta aacttataat tgaatataat taaaatgact gctctataga atataatttt
     8221 atacttctct aggacagtca ttttttatac taactattta tctccattat aacatctatg
     8281 gcacttttta cactatctac tacataattt gcactttgct ttacatcttc ccttgcctta
     8341 gacattgcaa aactatattt aaatccttct aacatgcaaa tatcatttgg agaatcccca
     8401 atagtagcaa cttcatctgg acttacattt aacgttttta ttacatgctc tatacttcct
     8461 ctttttgaca cacttttagg aactatattt aaagtatatg ttccagaaaa atatatttct
     8521 aagacttcac taaactcact ttttagataa agttctattc tggataactc ttccaaattt
     8581 tcagaacata cgcctatatt tcctataaga ttatcaaaac tacttctttt ttcaaaatct
     8641 gggtcaacta taaagtcact agaataatgt ctaaaagcag tggtatcact acccacaatt
     8701 acatcaccct tatatttaaa ataaagttgt gcatcacttg actcaaatct atcaatgact
     8761 ttttttacta aattatcatc tatcgttctt ttaaaaatta cattataatc tttatcatat
     8821 ataaatgaac cattttcaca tacataatat ccattcatat ctaaactatc tttgataata
     8881 ttatgtgctt gtttaaaaat tctacctgta gcaacattaa actgtactcc ctttttttct
     8941 atttcttcaa ttgctactat atcttctttt gttatggttc cattttcata tagagttcca
     9001 tctaaatcac aaaatatatg ttttatcata aattaaaagc ctccaaaaac aattttatat
     9061 tttactaata ctttaatcaa aataacttaa agcttttcta attgtattct caattcatta
     9121 agcaaatctc tagtagctct taatcttatc tttcttctat ccccattaaa tatatttttc
     9181 tttactattg tttttccatt tatatatatt gcagtataaa ccagtcccac aggcttctct
     9241 ttggttcctc caccaggtcc agctactcca gttgtagata tacctatatt agctttaagt
     9301 cctttagcaa cgccctcagc catttctata gctgtttgct cacttactgc tccaaactct
     9361 tccaaagttt ctctttttac tcctaatctt ttcattttag aatcattaga atatgttaca
     9421 caaccttcaa gaaatacaga ggatatacct ggatagttta taagagatga agaaaccaaa
     9481 cctccagtac aagattcagc cactgctatt gttagatttt tttctacaag tagcttagaa
     9541 acagcatctt ctaaagcagt ctccccttca gatatggaaa tatctccttc agtatataca
     9601 tatttaccaa ctctactttt tattttttct tctactggtt ttattaattt aaaagcttct
     9661 ctttcatttt cagcctttgc agttattctt attgtaactt ctaattcttt tgcatataaa
     9721 gcaactgttg gattagtctg ctcattaatt atatcgagaa tttctaattc tagatttgac
     9781 tcacctattc catataatct taaagtctta gatactagca tttcatttgt gaattgttgt
     9841 aagtaaggtt tgacactctc attaaacata gctttcattt ctcttggagg tccaggcaat
     9901 actattataa atttaccatc tttttttaaa attgcccctg gagctgtacc attattattt
     9961 tttagtacta tagcatcttt tggaaagtac acttgtttca tattattttc ggccattttt
    10021 ttccctgttt taacaaagaa agattccaaa acctctagtg aaggtttatg tagttctaat
    10081 ttctgaccaa agtattctgc agcagtttct tttgtcatat catcaccagt tggcccaaga
    10141 ccacctgtag taattacaaa atcacttctc tttaagcttt catcaaaaca ttctaaaagt
    10201 ctttgtttgt tatctccaac agtactctga tggtatactt ctattcccaa tgatgcaagt
    10261 tctttagcta aaaattgaga atttgtattt actatatctc ccaataatat ttctgttcct
    10321 acacttatta tttcagcttt caaaatttat cactccttga tttatagcca ttaagactag
    10381 tcttaagttc ataacaataa ttttagcaaa aataataaca taaataaagt taaaaaatca
    10441 tttaaacata tctttaaact agattattat tctaaaacca cacataatat aaacttcgta
    10501 tatagtggta ctttcatcct caactgttaa ttcatataat taagtatgta aataaaaagt
    10561 ttactttaaa ataataaaaa ttaaaatagg agtgtataat atgaaaatag cagttataat
    10621 gggtggaata tcatcagaga gagaagtttc tttaaattca ggaaaagaga tatataataa
    10681 ccttgataaa aataaatatg aagttgtaaa agtaataata gatgataaaa aagatatatt
    10741 tacaaagata ccagaagata tagattttgc aatacttgct ttacatggaa aatttggtga
    10801 agatggatgt atacaatcaa tacttgagac tatggatata ccatactctg gatgtggacc
    10861 tctttgcagt gggatgtgta tggataaaaa tataactaaa aaaatgctta gagattcaaa
    10921 cctacctact gctccatggg ttttagtaaa atcagttgat gaaatagact atgatgaaat
    10981 agataatata ggttatccag tatttataaa accaaactca ggaggttcaa gtgtagccac
    11041 attctttatt cattctaagg atgaagttga aaaagcagtt agaaaaggtc tggaagttga
    11101 tgaatttgta atgattgaaa aatatatacc aggtggagaa tatacttcat tcatattaaa
    11161 tggagaagtg ttccctacaa tatctataaa atctgattca ggattctttg attatgaagc
    11221 taaatactca gttgaaaaag gtgcaaagga agaagttgtt tacttagatg aagaacttca
    11281 aaaaagagta aacgaaattt ctgagacttg ttggaaaatt tttaattgca aagcatatgt
    11341 aagagttgat atgattataa gtgaaggtat tccatatgtt cttgaactaa atactcttcc
    11401 tggaatgact caaactagtt taattcctag aagtgctgct gctagaggta ttaagtacag
    11461 tgaactatta gataaactta tagaatactc tttaaattag atgaaataat ttgaggtggt
    11521 aatttgatat tttcaaatct aaaattaaat gataatgaac caatttatat acaacttaaa
    11581 aattatataa gtgatatgat atcaaaagga ctaattccag acaatagtaa gctcccttct
    11641 acaagggagc ttagtcagct tttacaagtt agccgaaatt ctgtagtttt aacttatgaa
    11701 gaattaaaat cagaaggatt aatctactcc atttcaggaa aaggtacatt tgttaaatca
    11761 aagaataagt cttcaaatac tacatggtca ttaaactggg attgtttaga gaatacatac
    11821 tctaaaaaag ccaatgaatt ggacattata aaaagtgaaa ttccttggag ctctgacctg
    11881 atttccttta aaagtatttc tccagatgga gatttattcg acatggaaga attaaaaaaa
    11941 tcctttttaa atagaatttc actagaagga cataaattac tcaactatgg atatgcacaa
    12001 ggttataagc cacttattga ctatttacta gaatatatga caaacaaggg tgctgatata
    12061 agtaataaag atatactaat aacaaatgga ttcacagaag gctttgatat tattatgtca
    12121 tcttttaccc aagaaaaaga ctacataata tgtgaaaatc ctactcataa tactgctata
    12181 aaaattatga aatctcataa cctcaatatc ttaggtgtag atataaatga agatggtttg
    12241 gacttcaata tgctagaaga caatttagtt aagtataaaa ataaaataaa gtttgcatac
    12301 attacaccct cttatcacaa tcctacagga attgtaatga ctcctgaaaa taggtacaaa
    12361 ttctataatc ttatgaaaaa atacaatata gcaattatag aagatggatt taatgaagaa
    12421 ttactctaca atagttctca tatatttcct atctgttccc tagataatat gaataatggg
    12481 gtcgtatata ttggtagttt ttcaaagatt ttatttcctg gaatgagaat tggatggata
    12541 tttgcagata aaaaagtaat caataagcta gaaagtgtaa aaaggtgcag aaacatacat
    12601 gtatcctttt tagaccaagg aattttattt gattatttaa gtaatggagg ttttgaaaaa
    12661 tacattaaaa aaatacgtaa attctatgga gacaaattta actttgcata taagtgtata
    12721 aaaaaatata taaaaacaga atatatcttg ggtgatggtg gtttgcatat atttataaaa
    12781 ctaaaaaata tagatactag agttttatta gaaaaatgtt atgaaaaaaa tgttatattt
    12841 atgcctggag acttattttt tacagataac tctggttttg atactttaag gctcggattt
    12901 tctaggttgt cttttgaaga cattgaaatt ggaataaaaa taattggaga aactataaat
    12961 gaaatgttct aggttaacta aatattgtta gcctagttta tttttatgag gtataatata
    13021 tatagtttat atgaatctaa tactggggga taatatgagt aaaaagtcaa aacgaagaaa
    13081 aattaataaa ttaaaattaa cgattgtaat aactgtatta tttcttgctt tattatctat
    13141 atatgagata ctgttctcta atcccaacaa aaaaggtaat ataaagagtc aagaaactat
    13201 gcaaaataaa aataatacaa cagatgattc taaaaataat caaagctcta ccaaaaagca
    13261 ggaagataat aatatcaaac ctactttttc atattcttca ataccagata atataaaaaa
    13321 taaaatgatt ggtaaatcta tgcctactga tgaacctata agctttgata gtctatctta
    13381 tttaaaactt acatattatg gatttgatga aaaaactcat caaggagaaa tgatagtaaa
    13441 cagtgaacta gctccagaag tagtggatat ttttaaggaa ttatacgaaa aaaaatatcc
    13501 aattgaaaaa attaagttaa tagatgacta tgatgctgta gatgaaaaat ctatgtctga
    13561 taataatacc tcatcatttt gttatagaac tatagctggt acaaatgtag tttcaaatca
    13621 tggtaaagga cgtgctatag atataaaccc attacagaat cctcaagttt ctggaaatga
    13681 tgttacacct aaagtaagta cagtttatgc tgataggtct agcacaaaat ttggaatgat
    13741 aaaaaaaggt gatgattgtt ataatgcttt tgtaagcaga ggttggagtt ggggtggcta
    13801 ttggaaaaac ccagattatc aacattttga aaaataatag attagaaata aaggtgaatt
    13861 atatgaattt aatgacattg gaaaatataa gtaaaagcta ttctgaaaaa aaattactag
    13921 aaaatatatc tcttggaata aatgaagggg aaaaaatagg acttattggt gtaaatggta
    13981 ctggtaaatc taccttactc aaaattattg caggagttga agaaagtgag actggtaata
    14041 taacaaaaac taatggtatt aggatagaat atctacctca aaatccaact tacaatgaaa
    14101 attcaactgt acttgaacaa gtatttaatg gaacttctga agaattaaag ctacttggtg
    14161 aatatcaaga aatcctagaa aaacttaatt caaactttac agaggaatta aataaaaaat
    14221 tattatcagt acacgaaaaa atagatgcct taaatctttg ggatttagaa agtgaagcta
    14281 agtcagtact tacaaagctt ggtataaatg actttaatca aaaaatagca gaactttctg
    14341 gtgggcaaag aaagagagtc tctctggcat cagcccttat tacaccttgt gagtttttga
    14401 tactagatga gcctacaaac catctagaca atgatactat agattggctt gaggaatatc
    14461 ttaattccag aaaaggttca ttgcttatga taactcatga tagatacttt ttagaccgtg
    14521 ttacaaatag aattattgaa ctggacaaag gtagattatt cagttatgat gggaattatt
    14581 caatattttt ggaaaagaaa atggaaagac tttcgctaga agcaagtatt gaagataaaa
    14641 gacaaaattt aataagaaca gagcttgcgt gggtaagacg aggagctcag gctcgtagca
    14701 ctaaacaaaa agctcgttta caaagatttg atgaacttat aaatagagat tcttttaaac
    14761 ctgatgaaaa tctcgatata tctgttgcat caagtagact tggaaataaa ataatagaaa
    14821 tacacaatat atctaaatca tttgaagaaa atactgttat agataattta gaatacactt
    14881 taactcgtac agatagaatt ggtataatag gcaaaaatgg tatgggtaaa tctaccctta
    14941 taaatatact taatggagaa atagagcctg acagtggata tatttcaatt ggagaaacag
    15001 ttaaaatagg ttgtttttct caagacgact ctcatatgaa tattgatatg agggctatag
    15061 actatgtaaa agaagctagt gactatatag aaacttcaga tggtactagg attacagctt
    15121 ctcaaatgtg tgaaagattc ctttttaatg gtactatgca atatactttg ataggtaaat
    15181 tatctggtgg tgaaagaaga agactacatc ttcttaaaat ccttatgaca gctcctaatg
    15241 tgcttctttt agatgaacct acaaatgatt tagatattga aacgcttaaa atattagaag
    15301 aatacctaga cgaatttaaa ggtgtagtag ttacagtatc acatgataga tatttcttag
    15361 atagaatttg taataaaata tttgcatatg aaggtaatgg aaaaattcat atctatacag
    15421 gtaattacag tgattatctt atttatagag agattcaagg aattgaattt gaagaatcta
    15481 aaagggaatc agttaaaaaa gatgactcta ctaccctaaa aaaagaaaaa cctaaaaatg
    15541 ataaaaagct taagttctca tataatgaac aaagggagtt tgaaaatatt gactctgata
    15601 tagaaaaact cgaatctaaa attgcaacac ttgatgaaag tactgcaaaa ttttcaactg
    15661 attttactaa actccaagaa attttagatg agaaatctaa actagaaaaa gaacttgaat
    15721 acaaatatga aagatgggaa tatttaaata atctagctga tgaaatagct aataataagt
    15781 aatattaaaa taatcttaag tttaataagt tttgctttaa atctgtaatt tataaatatt
    15841 ttaaaggaag tgtctcaaaa tgaacttttt agttttatga gacactcttt tttatgagaa
    15901 atcaaatatg ttttcatcat ttcaacaata caaaagaggc ttatctctat tttgagacag
    15961 cctctttcat aattaccttc taatttatat gctctaatat atctataata attcttatta
    16021 tatctatata tccagtcttt caatatagtg acaattaact tcaatatgat ttaaaaatac
    16081 taacaacctt tcataaagtg gatgagattc ttctccatat ttagcttcta ggttcttgcc
    16141 tatttctcta acagtccttt gaccatcaat ttgtaaaaaa acaaaactgg aatattcatc
    16201 tagttctatt tttttgtatt taggtattct agcttttaac tttctaaaaa acttttgaat
    16261 tttatggtcc tgtttttcaa gtatagtaac aacactattt tcagctactt catattcaag
    16321 tccatcagta attttaaata caatatctaa aacatcatta ctcttgctca ttatttttta
    16381 ctttagataa aacaataggt aatgctgtaa gcacaactaa aactactaat aaagcaattg
    16441 ccattgaatt tgttgcagca aatccacttg gtacctttgg agttacaact ccagttactt
    16501 gtaatattat acctattaaa ccaatgatag aaccaccagc aacaagacca gaagataaac
    16561 ttataccatt agaaacttta acttcttttt ctttttcagt tttagatact aattcaacaa
    16621 ataaacgaat taatgctcca actaatatta tagaagttgt tgcaattggt aaatagaatc
    16681 ctatagctat agtcattatt ggtaaattta atgcatatag aacgattgcc ataaatactc
    16741 caacaataat cataacccaa ggtaaactac ctgacataat accagaagtt aatgttgaca
    16801 ttaagtttgc ttgtggtaat gcaaattgta cattatcacc agttgctcta agttggtcag
    16861 aaagtaataa aattactcct acaacaacta taacaccaac tatacttgca attgtaaagt
    16921 aacgttgcat ttcgtttttg ctaccaccaa taatataagt tactttttga gattgagtat
    16981 aacctccagc tatagcaata gcaacaacta taaatgaacc aaataaaagt aatgatttat
    17041 tagcttctaa atcagtccat cccattataa caaacactaa tgttacaata actagagaag
    17101 ctatagtcat accagaaact ggaagatttg atgttccgat tgttcctgtt aaacgaccag
    17161 ctactatgac aaataataaa gataataaaa gagatatgat agctccaata attgccataa
    17221 caatgttacc agaaattaag aatgctgcta aaaatccaat aactactcca cctaataaaa
    17281 gtatcatttg tatagatgaa ccttcttctc cttctccagc acttgattta gctttaagtg
    17341 tttcttttat agaagcgatt atagttggta taagttttat tgcaccaata atacctccac
    17401 aaagcatcat accagcacct atatatttta cataactact agatattgct ccaacatcca
    17461 tttggttaag aggcattgca ggattattcc ataccatagc tccatcttta gccatatctg
    17521 taaagtatcc tattaaaggt gctataccaa agtttgataa aatacttcct gcaaacattg
    17581 ttaaagaaac ttcaagccct actataaatc caattcctaa aagtagagga tttacttcaa
    17641 tatcaaattt ccatttgtag aatttgcttc ctacaagact cattacattg tttgctacat
    17701 ttaagaatga accagttaat acagtaataa atccacttat tacaaatcca attcccatgt
    17761 acttaattga atctccacca gcctctgaag caacaagtgt ttcagatata gccattgatt
    17821 ctgggtacat aagtttacca tgttcttcaa ctattagata gttgtgaact atagcagcaa
    17881 ttcctatacc aaataaaact ccaccaactc caacagctaa gccttcaaag aatgtaattt
    17941 ggcttccaat taatataact gctggtaata cgaaaataaa tccacttgca acagattctc
    18001 caccacttga cataccttgg attaagtttt taccaagaat accctttgaa cgagcaaatg
    18061 ctccaacaaa agcagaacct attatagctc ctggaatacc tgcggcaact gtaagaccgg
    18121 ctttcattcc agagtaagtt gtagatgcgg caaaaatggc agctaaaata ataccgataa
    18181 tcagtacagc tacatttcca ccggttctgg acttatctgt gatatatgga acgtaatctt
    18241 ttccactaac ctcaccataa gcacccttag gtaatttttt gttcataaaa ttttcacccc
    18301 ttttcaattt gtatatatta taacatatat acatataaaa cttaactttt tattatttta
    18361 caaatagcga gtcaaaacta ctaaaattta accctatatt attaatttct acttttagat
    18421 atttttatgc taattttctt tttatttttc aattctacta aaaactatta agatataaaa
    18481 taaaaaaatt ttttaatgta aaattaattt tgtaaaaaaa atgctttaaa acacggaaaa
    18541 gtataaaaaa tacaataatc attcttataa ataaattttc accatagtaa atatatacag
    18601 aaaattcttg tttttatgca aattgattat tttataaaaa taagtgtcat aaagaaatgt
    18661 ctgaaatcac tttatgacac ctatcataat aaaaaattat tctggtaatg ctcttttatt
    18721 tacttttccg attggtgtaa ctggtatctt atctatcctc attattattg ttggaatcat
    18781 atatggaggt agatgctcat ttaaatattc tctaatagtt ctttcattta tcttaccact
    18841 atcttcctca tcaattacat aatatgcaca tatatacata tctccatcat catttgttct
    18901 cgatatacaa acaacacttt taatttctgt aatattattt attatatttt caatctcata
    18961 tagctctatc ctaaactcat caatttttac ttgattgtcc atccttccaa tataatctaa
    19021 atttccatca tctaaccatc ttactaaatc accagttcta tacattaact tattatgttc
    19081 acaagttttg taaggattct ctacaaatac ttgctctgtt agaccttttt tatttaaata
    19141 tcctcttgat atttgaatgc ctgatagaca tagctctcct ggtgctccaa ttggacatag
    19201 tttgttcttt ttatctaata catatgcata agtattaaat ataggttttc ctattggtat
    19261 ttttccatat tctctgtcca catgaaaaga agtcgataca acagtaaatt cggttggtcc
    19321 atactcatta tatagttcat aattacgctt tctatatcct tttaatcttt ctcctccagt
    19381 ctgtacaact ctaagtgaat tattattgca attttgtata aataaccttg caaatgctgt
    19441 tggcaatgct agtatagtta tgtcattttc ctcacagtat tcatttactg tatattcatc
    19501 aaatttagct tcttcaggca taatatctat tgatgcacct acaagcaaat aagggaatag
    19561 ttgtttcatt acagcatcaa aactgaaact taagtatata ccacatatat cattttctgt
    19621 agcgcttata gattttacgc tataataaca catatttact aaactcttat gttctatagc
    19681 tatggtcttt ggcctaccag ttgaccctga agtagatatc gtatatgcac aatttgatga
    19741 tgttattttt tcagttaatt catatccaag cttaaacatt tcttcatcat ttatatcgag
    19801 tactatggta ttctcattta caaccttact tttgagttca tctgtagtta tcactacttt
    19861 tgcattacta tcttcaagaa ggtaattcaa tctttcttct ggattacttg tgtctattgg
    19921 aaaaaatgct ccccctgatt tcataatccc tattgctgta ataattatgt taatatctct
    19981 tggtaacatt acagctacta tgtcttcact ttccactcct atatgattta aataacctgc
    20041 aagtttatca gttacttcat ccaattcttt atatgttaac tttctatttt catccctagc
    20101 agctatatta ctaggtgtta ccttaacttg ctccctaaat aaatcaacat aacttttatc
    20161 atagggtatt tcatattgat tattattaaa gtcttccaat aatacttttt cttcttcctt
    20221 acatacaaat tctaaatctt ctattaaaat atcattatct tctattattt gctctattat
    20281 ataatcaaat tgatttaaaa ttcttctaat agtttctctt ttatatatag atggtttata
    20341 ctttattttt attttaagtg ccctcttagt atatactatt acagataaat catatccttt
    20401 tttttctatg tcataatcat atgataaatc aaaactttta gctataaata ttgtgtttat
    20461 aagcttagta tttagatgat ttttattctc tatatctgat aacaaatagt atccatactc
    20521 taaagacctg ctccactgcc tttttagctt ccttacaaca tctataaatt tttcactttg
    20581 tctaactctt actcttattg gaattatatt cattgtttta ccaaaaacta catcatcttc
    20641 atatatatac ttttgaagta taaggccata tatagtttct atgatagagt ctatgctaat
    20701 attgttcact ttagaatatt gaattaacct ttcatgtttc tcaatattca tcttaaaaac
    20761 ctcatctact acttcatcag aacaatcttt ataaaaaaaa tcatttggaa tttttgctat
    20821 attcgtataa tcatctaaca aatccatcca ataattcata gctcgttcta aattaacttc
    20881 atacatatca aaattccccc ttactactaa gtctatagaa aaaattcttt tggaatttta
    20941 ttttcacaat atataaatat attaataata catatgcaag ctctaaaaac atgagcttgc
    21001 ataaaattac acttattatg actactaaat tcgtaatatt tacaatgttt aaaatatatt
    21061 ataacacata tatgatttta tttttctaca tttatataat atataaatta caaatagtta
    21121 tttaactaat atataacaat accttttgga atatgatata ttatattaat taaattttta
    21181 taagataagt tatacttata ggagggaacc atataaatga atatttatgc tcataggggg
    21241 tttagtggaa agtatccaga aaatactatt ctagctttta aaaaatgttt ggatatggat
    21301 atatatggaa tagaacttga tgtccacaga actaaggatg gaaaaattgt agttattcat
    21361 gatgaaaagg ttgatagaac ttttaatgga catggttttg taaaagactt tactttaaga
    21421 aaattaaaaa ctctaaactc atcttttgaa ggatatcaaa gcaataaaga atgtaaaatt
    21481 ccaactttag aagaagtatt aattctaatt tcacctacag accttatttt aaatatagaa
    21541 ctaaaaactg acaaaataaa ctatcccaat atagaaaaag acgttttaga gcttatttta
    21601 aagtataata tgaaaaatag agttcttata tctagtttca acagcaatag cttaaaaaac
    21661 tttcataaat tagaccctag tgtaaaaaca ggattattgt gttatttacc tataaacaat
    21721 gtagtcaatt ttgctaaatt tctgggtaat agttaccttc atccacctct agttttagta
    21781 aatgaatctc taattgaact ttgtcacaaa aatctacttg gtgtcaatgt atatactgta
    21841 aatgaagagg atgatatact acactgccta aaactcaatg tagatggtat ttttactaac
    21901 tatccagata ttgcttcaaa tcttttacac tcaaaacaat acagctaatt atacaatagt
    21961 ttgtactcct tgacttttat ataattaata tgtacaattt tattaaattt atcttggagg
    22021 tataactatg gataacttat taaatgaact tgaagcttta aagagtaatt tagataatct
    22081 accaatagaa aaaatagaaa attatgattt gagtaaaact gctttattta taattgatgt
    22141 taataatggc tttgcaagac aaggtgcatt atactctcct agagtagagt ctttaataaa
    22201 gccaattgaa atgtttacaa aaaaaatatc aaacaaatta aataaagtaa tagcatttac
    22261 tgattctcat actcctaaat caatagaact tttgagttat cctgttcact gtttggaaaa
    22321 tgatgtagaa agtgaattgg ttgatgaact taagtcgata gaaaacctac aaattcttcc
    22381 taaaaattct actaatggat tttttgcatt agaaaatttg gattttgata atatagataa
    22441 tataattata gttggcgatt gcacagatat atgtatatat caatttgcaa taactctaaa
    22501 atcatatttt aatcaacata atattgaaaa aaatatagtt gtacctatga atttagtaga
    22561 tacttatgat attcctaacg ttcaccctgc tgagatttta aacttagtat ttttcaacag
    22621 tatgattcaa aatggagtta atgtattaaa agagattcgt taagcctcta atactttaca
    22681 tcccatataa tacaattatc agatatttat ttttaataaa atttctaata gaaaaacaca
    22741 gttttagtga gaagattaca atttaaaaca ctatctatta ttactaatct tcacaatata
    22801 aaaaatcgca gtttagatat tccttataca aatttataag gaataataaa ttgcgatttt
    22861 tatttttaag tagtacaatt aattgatact acttcagtat ttaaattcat tttttaataa
    22921 tcatacttat tcgtagtttc gcacattact acctttattt ttttattaaa catattctta
    22981 tattctaata aaaactcatt tagttctgaa tccataagat ttatttttct atcactacaa
    23041 agcattatct tttcttggtc gattattaca ttaatatcat ttaagtcatt ttttataaat
    23101 gttgttatac aactctttgg ttggattttt tctccatatt tctcataaaa tcctgcactc
    23161 ttcttaaatg actctaaatc ttctgtagat actttgctgg aagtattatt actcataaaa
    23221 cctgctgtag aagggtcgat tccaaagttt ctaagttgtt tttctacttc ttcagtgtcc
    23281 ataccatatc tctccataaa ttgcttttgt attttcataa atttttcttg agatatgttg
    23341 ttatctctca tataatcgaa tatttttctt gaaaactcag ccatagacat ctttccatcc
    23401 ataacagaaa aatataagtt atatatgtat tgctcaaatt tatctacatc agaatcttta
    23461 actttgttct ttagttcgtt aaagtctatt attctatcat cagacatttt tcatcctcct
    23521 agctaaattt ttacttgtat aagattatac catatttcta ttatatttat atacacacta
    23581 tttttttaat ttaacgattt tttctgtagc atctaattcg cacattctgc ccaaaggaat
    23641 agttatcatt gggatacaat gtcctgactg aagattatat atagaaggtt tattgacctt
    23701 atttgcaaat tctattagta attccatcaa atcattttct tcaatacaat ccttaaaatc
    23761 tccaaaaatt ataccattac attcctcaag ttttcctgaa gaatatagtt gtgttaacat
    23821 tctatccagt ttatattgag gttctccaat ttcctcaata aacaaaattt tatcttttgc
    23881 atctatttca tattttgttc ccattgtggc aattattaag gataaatttc ctcccattat
    23941 tattccctct gctttacctt cacatacagt ataaatcttt tcttcttttg gattttctaa
    24001 atataattca tctttaaatt ctaaagcatt taccaatgat ttataggtaa atttatccca
    24061 tttacctaca tttgctgcca ttattccatg atatggtaaa agaccacaat ttttatagaa
    24121 agctaaattt aaacctgtta tatcagaaaa acctataaat ggttttggat tttgctttat
    24181 tatatcataa tttattttat ccaatattct tgttgttcca taacctcctc ttaagcacat
    24241 aattatatct acatctttat ctaaaaacat atcttctata tcccttgctc taatatcatc
    24301 attaccagct aaataacctt tataatttaa actacaactt tcagaaaact taacttcata
    24361 accataactt tctaaattaa actttatttc ctccaaactt gtatttctta atggcccaga
    24421 tggcgcaatt attcctaaca tttaaacacc tcttcttata aaatgatata ttcttctcca
    24481 tactaaatat ctctaatata attataagat attttatttg tacttgatac ataataatga
    24541 tttacataaa ataatataat taatgaagaa aaataaattt ttaatatagt taggatgtgt
    24601 tttatgctta atataaaaac tctttgtcat gagataaatg attgggttat aaatataaga
    24661 agagatttac ataagactcc agaacttgga ttagaagaat ttcaaactaa gaaaaaaatt
    24721 attaaatact taaatgaaat aggtataaat tatattgaat ataagaatca tacagggata
    24781 actgcataca ttaacgtgag tccaaacttt gaaacagttg ctataagagc tgatatagat
    24841 gccttaccta taacagaaga acttaattat tcttataaat ctattaatat tggcaaaatg
    24901 catgcatgtg gtcatgatgc acacactgct attttactgg gtacttgtaa tatactattt
    24961 aaattaaagg attacttaaa tgtaaatgta aaattctttt ttcaacctgc cgaggaaact
    25021 attggtgggg ctcaacttat gatagaagat ggatgtcttg aaaacccaaa tgtaaaatat
    25081 atatttggac ttcatgtaaa tcccaatatc aacaaaaatc taattgaatt aaaatacaac
    25141 actctaaatg cttccaccga tacacttcaa ttaactgtac atggttcaaa atgtcatgga
    25201 gcttatccac atcaaggtat tgatgctatt gttatatctg ctcatattat tacagctcta
    25261 cagacaatcg ttagtcgaaa cacaaatcca actgattctg tggttatttc tctaggagaa
    25321 attgaaggtg gaatcaagga aaatatagtt tgtgataaag tggttataag gggtacttta
    25381 agaaccttga ctcctgaaac tagagagttt tcaaaaaaac gaattagaga aatttgcgat
    25441 tttacttgta aaacatttgg aggaagtatt tctgttgaaa ttgaggaagg ttatccagct
    25501 cttatcaatt caaaccatct agtagattac gtcaaacaaa atgctgtaga attgtttgga
    25561 gaagaaaata ttatccttaa agactcgccc actcttgggg ctgaagactt ttcgtatttt
    25621 ctaagacact gtgaaggagc tttttaccac cttggttgtg caaatcgtga aaaaaacatt
    25681 actttccctt tgcatacctc tacttttgat attgacgaag attgccttat tactggtgtc
    25741 atactccatg taaaaaatgt attatcattc taactattaa tatttttatt tattatttta
    25801 tattgaccct cacatgatgt catgttatac cctaaaatta caaatgataa attaaatagt
    25861 tggaggtaat attatgaaag aaatattaat aaatcctata ggtaaaatat gtattcaagg
    25921 cgaagaggtc tttattaaat tagacaaaaa gtatgtacct gcattaaatg aactcgacga
    25981 tttcagtcat ttaaatgtat tttggtgggc tgatgggttt gataatcctg aactaagaag
    26041 tattttagaa accccaaaac cttataaaag tggaccagac gttataggaa tatttgcaac
    26101 aaggtcacct ataagaccca atccaatagc attgactgct gtacaaatta tcaatatcga
    26161 ccatgaaaat ggcataataa aaataccata tattgatgct aatgataata gtcctgttat
    26221 tgacttaaag ccatatactc caagcgttga tagggttgaa aatccgtccg ttcctaaatg
    26281 gtgtagtcac tggcctaaga gtgtagaaaa atcaggagaa tttaattggg aagatgaaat
    26341 tgaattttaa attacttttt tcttgatttc attaacttgt tttccacatt tattttattt
    26401 acacttacca agattttatg tcacatatac
//
LOCUS       DAELJO010000032        25939 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELJO010000032 DAELJO010000000
VERSION     DAELJO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.032"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            666..1688
                     /locus_tag="KPB88_001211"
     CDS             666..1688
                     /locus_tag="KPB88_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0053508.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNAELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1982..2902
                     /gene="pyrB"
                     /locus_tag="KPB88_001212"
     CDS             1982..2902
                     /gene="pyrB"
                     /locus_tag="KPB88_001212"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG0053509.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2941..3633
                     /locus_tag="KPB88_001213"
     CDS             2941..3633
                     /locus_tag="KPB88_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG0053510.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3626..4528
                     /locus_tag="KPB88_001214"
     CDS             3626..4528
                     /locus_tag="KPB88_001214"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0053511.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4613..5197
                     /locus_tag="KPB88_001215"
     CDS             4613..5197
                     /locus_tag="KPB88_001215"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG0053512.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5412..6338
                     /locus_tag="KPB88_001216"
     CDS             5412..6338
                     /locus_tag="KPB88_001216"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG0053513.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLEENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6390..7280)
                     /locus_tag="KPB88_001217"
     CDS             complement(6390..7280)
                     /locus_tag="KPB88_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0053514.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7468..8499
                     /locus_tag="KPB88_001218"
     CDS             7468..8499
                     /locus_tag="KPB88_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG0053515.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            8793..9668
                     /locus_tag="KPB88_001219"
     CDS             8793..9668
                     /locus_tag="KPB88_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG0053516.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9686..11176
                     /gene="cls"
                     /locus_tag="KPB88_001220"
     CDS             9686..11176
                     /gene="cls"
                     /locus_tag="KPB88_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0053517.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRLLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11369..11653
                     /locus_tag="KPB88_001221"
     CDS             11369..11653
                     /locus_tag="KPB88_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG0053518.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTIGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11686..13314
                     /gene="groL"
                     /locus_tag="KPB88_001222"
     CDS             11686..13314
                     /gene="groL"
                     /locus_tag="KPB88_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG0053519.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKENTTIVDGS
                     GDKKSIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13576..14316
                     /gene="larB"
                     /locus_tag="KPB88_001223"
     CDS             13576..14316
                     /gene="larB"
                     /locus_tag="KPB88_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG0053520.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDIGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEELSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            14400..15134
                     /gene="larC"
                     /locus_tag="KPB88_001224"
     CDS             14400..15134
                     /gene="larC"
                     /locus_tag="KPB88_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG0053521.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKFKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDEFEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15164..15583
                     /locus_tag="KPB88_001225"
     CDS             15164..15583
                     /locus_tag="KPB88_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG0053522.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15643..15744
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15965..17500
                     /gene="guaA"
                     /locus_tag="KPB88_001226"
     CDS             15965..17500
                     /gene="guaA"
                     /locus_tag="KPB88_001226"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG0053523.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(17577..18623)
                     /locus_tag="KPB88_001227"
     CDS             complement(17577..18623)
                     /locus_tag="KPB88_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0053524.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(18700..18915)
                     /locus_tag="KPB88_001228"
     CDS             complement(18700..18915)
                     /locus_tag="KPB88_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0053525.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(19349..19750)
                     /locus_tag="KPB88_001229"
     CDS             complement(19349..19750)
                     /locus_tag="KPB88_001229"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053526.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            19915..20127
                     /locus_tag="KPB88_001230"
     CDS             19915..20127
                     /locus_tag="KPB88_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053527.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            complement(20422..20640)
                     /locus_tag="KPB88_001231"
     CDS             complement(20422..20640)
                     /locus_tag="KPB88_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053528.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            20759..20947
                     /locus_tag="KPB88_001232"
     CDS             20759..20947
                     /locus_tag="KPB88_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053529.1"
                     /translation="MNNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNIRT
                     ALKIARILNTNCEKLFNE"
     gene            20989..21783
                     /locus_tag="KPB88_001233"
     CDS             20989..21783
                     /locus_tag="KPB88_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBG0053530.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEINHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSAYLDLKNENRKCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NKMEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADS
                     VASSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLG
                     VIETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            21867..22247
                     /locus_tag="KPB88_001234"
     CDS             21867..22247
                     /locus_tag="KPB88_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053531.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGSKPKICCICTKEVE
                     YEKLEQQSVLENTEIKEVCNQMKIKKLALATGEVFENIELKEFENKGECLPPNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            complement(22237..22389)
                     /locus_tag="KPB88_001235"
     CDS             complement(22237..22389)
                     /locus_tag="KPB88_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053532.1"
                     /translation="MVIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQT
                     KKGPKK"
     gene            22448..22645
                     /locus_tag="KPB88_001236"
     CDS             22448..22645
                     /locus_tag="KPB88_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053533.1"
                     /translation="MAINDNINKILRDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            22684..22890
                     /locus_tag="KPB88_001237"
     CDS             22684..22890
                     /locus_tag="KPB88_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053534.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            23303..23620
                     /locus_tag="KPB88_001238"
     CDS             23303..23620
                     /locus_tag="KPB88_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053535.1"
                     /translation="MKSLIIVRNSVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEELERDIPID
                     VQI"
     gene            23703..24212
                     /locus_tag="KPB88_001239"
     CDS             23703..24212
                     /locus_tag="KPB88_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG0053536.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRAKKNTLDKLKSNIKDCMELLETKKVETIL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            24222..24830
                     /locus_tag="KPB88_001240"
     CDS             24222..24830
                     /locus_tag="KPB88_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG0053537.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVVLDDNIVQIGNRFYVEATATLIDAETGEKISVKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            24840..25721
                     /locus_tag="KPB88_001241"
     CDS             24840..25721
                     /locus_tag="KPB88_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0053538.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILKELIENGYVTRKPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYYESYIGVITPNNFLQLLSYLDDGMEADVIIRAVDEAVGSGVKNYKYIKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNNKTVNTHNVSKNKFANFNQTFTQYD
                     EKELDEIIKKSQKEKFK"
     gene            25782..>25939
                     /locus_tag="KPB88_001242"
     CDS             25782..>25939
                     /locus_tag="KPB88_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG0053539.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTE"
BASE COUNT        10422 a         2724 c         4663 g         8130 t
ORIGIN      
        1 gaatttcatt taaaatggaa tttcaaagtt gctatagtaa gtagtttaag cattcttctt
       61 atattaatct ttgagaataa tgtatttaaa aaagttagta aaaaatttaa ctattgtata
      121 ttaatgatag ttgctattga tatattatct aaaaaaatta agtatgaaaa gattactgct
      181 aggacaatct tttcatactt aatttttaat atttatattc tccaaaattt tcatcttttt
      241 aacttagaat ttgagaatca ctattaggaa ataggctttg atttatgtag ggttaaaaat
      301 ttcacagaat atctaacttt atactaaatc catcaaaaaa tatacgccat acaaaagcgc
      361 ataaaaaaac acataaaaca atatttatgc gcactttaac ctcgaatggt acaaaattct
      421 ttacttttac agacccatat tatataatta agtaaataat gtatatttag gtgacatatt
      481 ggataagcta atttatttat tttattaata taataatact ataaaagtta tataaagtgg
      541 tttacaaaaa agttacagat agagctttta ttaatgtatt gtgtattagt attttatcct
      601 taggtacact tcacctacaa aatagaatca taagataatt atgggaaaat tttaggaggt
      661 ttatcgtgaa gaaaaagata ttaataccag tatttgcatc agttatggca ttatcagtaa
      721 gttcaattgt aaatgcagat gaagtaaatg attcatctca aaataaggat gataaaacaa
      781 atgctgaatt aaatttaggt gaatataaag aagtaaaata taaagttgca aaaataaaag
      841 atggagtagc tataaagata agagaagaag gtcaagttca aaacatagct tattctggag
      901 atgaatttac ggttttagga actcaaggag aatgggttaa ggttaaagtt gaagatggcg
      961 aaggttggtt agctactaga tatgtagata tatctgaagg tgtaggatat actaacgctg
     1021 ataaagtaaa cttaagaaaa gataagagtg aaagttcaga agtaattgaa gagctggaaa
     1081 aaggaagttc tttattggtt ttggaagata atggagattg gttaaaagtt aaagatgggg
     1141 aaacagaagg atatgtaaaa tcatcttata tatctgataa agctcctgta atagaagagc
     1201 cacaagtaaa cccaagtatt gaccaaaatg tagattctac aaataataat caacaaaaca
     1261 atgcaaatac taatcaacaa aataataatt caaatgtgcc aacagctaac tctaatgcag
     1321 ttcaagctgt acttaacttg gcatattcta agcaaggttg tccatatgtt tggggagcag
     1381 aaggaccaaa cacttttgac tgttctggat ttacacaata tgtgtataga aatgcagtag
     1441 gaaagaatat accaaggaca tctaaagcac aatctaaata tggacaaact gtaagtaaag
     1501 caaatttaca accaggagac ttagtattct tcacaactaa tggttctggt tctgttagtc
     1561 atgttggaat atatgtaggt gggggtaata tgatacattc accatcaaca ggaaaaacag
     1621 ttagtgtaac tagtataaat tcatcatact atacagcaag atttgttaca gcaaaaagaa
     1681 tattataatc ggtttgtaat atgacaaatt gataaataca taaatatata aacgctctat
     1741 attatattta atttaatcat gaaaacaaaa aatgattata ttttaaatgt actatagagc
     1801 gtttttgtat cactttaaat ctaatcaaga ttcaagttaa tatgactgtt aaataggagg
     1861 cttttcatat gatgattatt tgcgcaatcc cttgacagga tagggcaaag gtggtaagct
     1921 actaacagta caaaaatact tttaatttta gttcagagag gctaaaaaag gaggacgaaa
     1981 gatgttaaaa tcaagaaatt taatccaacc agaagacttt tcaatagaag aaatagatga
     2041 aatattagaa ttagctcaaa aaataattga caatccgtca aaatattctc gtatatgtga
     2101 aggaaaacta ttagctacat tattttatga accatcaaca agaacaagat taagctttga
     2161 atctgctatg aacagattag gaggacgtgt agtaggcttt tcagagccaa attcatcctc
     2221 tgcatcaaaa ggtgaaactc taggagatac aatgaggata gtatctggtt atgttgatat
     2281 aatagctatg agacatcctc aatcaggtgc agcttcagaa gcagctagat atacggaagt
     2341 gccatttata aatgcaggag acggcaaaaa ccagcatcca actcaaacac ttacagactt
     2401 acttactata aaatcattaa aaggaacttt agaaagccat acaataggat tatgtggtga
     2461 tttaaaatat ggaagaactg tgcattcttt ggttaaggct atggcaagat ataaaaatac
     2521 aaagtttgta tttatagcgc ctgaagagtt aaaaatgcca gattatataa aagaagctat
     2581 aaagggacat gcatattatg aaactaataa tttggatgat gttataggaa gcttggatgt
     2641 tttatatatg actagagttc aacaagaaag atttgaagat aaatcagagt atgaaagact
     2701 taaaaactat tatattttaa ataaagctaa attagaaaag gcatctaaag atatgttagt
     2761 aatgcatcca ttaccaagag taaatgaaat agacatagat gtagatagtg atgatagagc
     2821 tgtatatttt aaacaagcta aatatggaat gtatgttaga atggctttaa taataaaact
     2881 tttaggtata aatgaagact aatagttatc atataatagt aaaacaaagg agattttggg
     2941 atgtataaaa ttctggaaaa tatatatata ggcgaagata tgtatagaat gaaagtaaaa
     3001 ggtaattttg aaggtaaaat gggacaattc tatatgttaa gagcatggga tacttatcca
     3061 gtactttcaa gacctataag tatacatgat atagatgaag aagggataac ttttctttat
     3121 aaagtagtag gagaaggaac tcaaattttg agtaacctaa aggtaaatga tactataaaa
     3181 ttggaaggcc cttatggtaa tggatatgct aaggttgatg gaaaggtagc tctagtagga
     3241 ggaggaatcg gtgtagctcc actatatttg gttgctaaaa atataaaaaa ttgtgatgca
     3301 tatcttggct ttagagaaga tgttatatta gaagatgaat ataaacaagt atgtaataaa
     3361 gtctatacaa cagtaggaaa tacttttgta acagatataa ttgatgttga gaaatatgat
     3421 tatatattaa catgtggtcc aactccaatg atggaaaaat tagttaagat ggtagaaggt
     3481 actaaaacga gaattatggt atctcttgaa aaccatatgg cttgtggagt aggtgcctgt
     3541 ttagtatgta cgtgtaaaac taatggagga aataaaaaga cttgtaaaga tggtccagtg
     3601 ttctggggag aggacgtgat ttttaatggg taatttaagt gtgaaatttg gaagtgtaga
     3661 atttaaaaat ccagtaataa tggcgtctgg aacttttgga tttggaaaag aatataatga
     3721 gatttatgat atacaaaaac ttggtggtat aagtagcaag ggtcttactt taaataaaaa
     3781 acctggtaat aatggtatga gagttcatga gacaccatcc ggtatgatga atagtgttgg
     3841 gcttgaaaac cctggagttc aaggatttat agactatgaa ttaccttttt ttagtaaact
     3901 agacttagtc agaatagcta atgttggagg tgggacatta gaagactact tattaggggt
     3961 tcaaatgtta aatgacaagc ctatagacat tattgaatta aatatatctt gcccaaatgt
     4021 caaagctggt ggaatggctt ttggtataaa aaatgaagtg gcaagagaag ttgttcgtga
     4081 ggtaagaaac ataacgaaac tgcctttagt aataaaatta tcacccaatg cagaagatat
     4141 tgtaggaatg gcaaaagttt gtgaagaaga aggtgctgat ggagtttcat tagttaatac
     4201 ttttaaagca atggcaattg atataaaaaa tagaagacca gtatttgaaa atgtatatgc
     4261 tgggttatct ggaccagcta taaaacctat agctttgaga atggtacatg aagtgtgtaa
     4321 aaatgtaaat atacctgtaa tgggaatggg cggaataact aaagccacag atgctataga
     4381 gtttataatg gcaggagcta cttgtatcca agttggaact gcaaatttta tgaatcctag
     4441 aataggtatt gaaataatag atggaattaa tgaatttatg gatagagaag gaataaaaag
     4501 tttagacgaa ataagaggaa ttatataagg tagaataaat agtattttaa agataaaaaa
     4561 tatagattaa ataaacaata attttataac attaacatgg aggaaaaata atatgagtaa
     4621 agtagatgta gtggatatat taaagaaaag tgatgcattg ttggagggac attttttatt
     4681 atcttcagga aaacacagta atagatacgt acaatgtgca aaagtattaa gatttccaga
     4741 atatgctgct gaggtattaa gtacagttgt tgaacaaata aaagacttag atatagactt
     4801 agtagtagga ccagctatgg gtggagtaat agtttcttat gagttaggaa gacaattagg
     4861 aaaagaagct gtatttactg agagaaaaga caatacaatg gagttaagaa gaggatttga
     4921 agttaaaaaa ggagcaaaga taataattgc tgaagacgtt gtaactactg gtaaatcaac
     4981 tatggagaca aaaagagtat tagaagcctt aggtggagaa gttgtaggtg ttgcatgtat
     5041 agcagataga actaatcatg atataggtat gcctatatat agtgctataa aacttgatat
     5101 tcaagtttat gaatctgatg agtgtccttt atgtaaggaa ggaaaattac cagttgttaa
     5161 gcctggaagt agagagttca aggaattagg gatgtaataa aaacttaatt atttatagtg
     5221 ttacttaaaa aatgtaaatt ttttagagta aaaatttgta atataacata taacttaaaa
     5281 ataaaaagca gttagcctta taaatgaagg ttagctgttt tttttgttaa aaatatattt
     5341 acaaaataag agatagtagc agtacaatat aagtataagg taaaagtatt atatattagg
     5401 gaggggtaag tatgaaaaat caattaaatg aaaatcataa aaaggttgta ttttttggag
     5461 taggtgctgt aggagctact tttgcagaac aattttttaa ctctaaatat gattttaaaa
     5521 ttctttgtga taatgaaaga aagaaaagat atttagaaga aggatttata ataaatggaa
     5581 aaaggtatga ttttgattat gtaactaaag atgagtataa acaagaggct gattttataa
     5641 ttataggtct aaaatataat aatttagaag aaaatataaa agaattagat ggattagttg
     5701 gaaaaaatac agttataatg tctctgctaa atggagttga tagcgaagag ataataggag
     5761 aaagatttgg aattgaaaaa atggtatatt catatgttac caatatagat gcaaagaaaa
     5821 tcaataataa tattatacac actactaatg ggataattgt atttggtaac aaagataata
     5881 gtgaagatag aaaaactaat ataataaccg aagtctttga tgatgtaaat atagaatata
     5941 ctttatcaaa agatattcaa cgagatatgt ggtggaagta catggttaat attggtgtaa
     6001 atcaaacttc agctatactt ggtgcacctt atggagtttt tcagagttct gagcatttaa
     6061 gagaattagc aaaatctgca atgagggaag ttgttgctat agcacaagca aaagacatat
     6121 ctcttacaga agatgatgtg gaacattcat tacatagaat actagaacat tcaaaagaag
     6181 gaagaacatc aatgcttcaa gatgtggaag ctcatagact tacagaagta gatatgtttt
     6241 ctaagaatat ctgcaaactt ggaaaaaaat ataacatacc tactcctata aatcagactt
     6301 tcttttatat gataaaagta attgaaagta gattttaaag ggtctttgta agaaatattg
     6361 ataattattg attttaaatt ttattaatgt tatatactat ttaagttact aaatattttt
     6421 aactcttcaa tgaaaatagt tgctgctttt gataaagtta tatcttttgg aaatatatat
     6481 gagaaatgtc tgttgtagct gcttccaagc tctatgacag acagctcatt gtttagaaca
     6541 ggtaagcttg tcactagatt agagactatt gtaattccta aattatttct aacagattct
     6601 ttgacagcat aattacttcc aaaaaccatc atactttttg gaataatttc tttaacactt
     6661 aaaaacatat ctaaataatc tctggttcca gaaccatcct ccctgactat ccatttttgg
     6721 ttttgaagtt tatcaaaact aaaatctttt aataaatgac tcttgtatgg aagtgctaaa
     6781 accatcttat cttcaaaaaa atattcttga ataaatgaag gggaagagca agtgccttca
     6841 ataagaccaa tgtctaaaat ataatcctta acatgagaag atacgattga tgtatttttt
     6901 ataaaaactt ctacatctat gtcaggatac tttttagaaa ataatgctag aaaatttggt
     6961 aaaatatact ctccaatagt taaacttgca ccaatcttta agtgaccagt tatagcatct
     7021 gaaacttggc ttacatcatg ataagtaata tttaaaagat ttaatatttc tttagctctt
     7081 ttgtacaaga tttgtccact ttctgttata aaaatggttt tttgtttaat agacctgttt
     7141 ataagcacaa ctttaaaata attttctaga ttttttatgt gtttacttac acttggttgt
     7201 gatagattta aatattcccc agctttagta aagtttttgt attcaacaac agctacaaat
     7261 gtttttaatt cttcaaacaa ttttttgtct cctttaccta atgaaatttt acatttttta
     7321 tatactttct acagataatt atactatata taataaccat taaaaacatt aatcattacg
     7381 atattatata tttattttac ttatggttat tacaattata tactattgct atgaagaaaa
     7441 ctatattaat tataaggtag gtatattatg ttaaagaaaa aaagcagttt gacattaaaa
     7501 actataaggg aaatattacc aggactgttt gtatcagtat tagtaggtta tattagtata
     7561 tttatttcta ttttaattcc taaggtaggg gcagcatcaa tttctatatt tttaggtatg
     7621 tttgtaggaa acctattttt aaatcaaaag gtatttcaaa aaggttataa attttcagag
     7681 acagatttgt tatcatattc aatagtacta ttaggagcaa cactcagtgt atctacttta
     7741 atagacttaa aagtttcagg aattttattt ataatattgc aaatgacaat aacaataata
     7801 gctgcacttt atataggtaa aaaactagga tttgaagaaa actttagatt tctaatggca
     7861 agtggaaatg cagtatgtgg ttcttctgca ataggagcaa cagttccagt ggttaatgcg
     7921 acagataaag aaaagggaat tgcagtaact attgtaaatg ttactggtat atttcttatg
     7981 tttttacttc caataatatc tcaattttta tatagccatg aacttgtaaa aacttcagca
     8041 atgataggtg gaactttgca atcaattggt caggttgtag caagtggagc aattgttgga
     8101 gaaaatgtaa aagacttagc tactattttt aagatagtaa gagtaatatt tctagttgtt
     8161 gttgtattag tatttggaca tatgaaaaat caatctaata gggaaattct tgaagaagaa
     8221 aaggatgaga taaaaaagaa aaaagtgact ataccatggt atgtaattgg attctttata
     8281 acttgtgcac ttttttcaat gaacattata ccacaagaag tatctgtgtt gtgcaaagaa
     8341 ataagtaaca aactagaaat aatagcttta gcagcaattg gattaaaggt taatgtaaaa
     8401 gatttagtga aacaagggaa agaagtttct ttatatggtt tatttgtagg tatagtacaa
     8461 gttgtatcag ctgtagtttt aataaaaata tttatatagc ataaaaatag tatttgtaat
     8521 attaatttga ttaattaaaa taatatgata ctaataattt ataatataaa taaaaaatga
     8581 atttcgataa gtttaagtgg gtataaactt atagaaattc attttttatt taaggtaaaa
     8641 atttaaagta aaatattatt ttacataata ataaccttat aaattttgaa attacttatc
     8701 tttgtgttaa aataagtaaa atgcacatat tagagctaag tttttaagta aataaacaaa
     8761 atgaaaatat aaatattaag gagatttggt atatgaatgt ttatgaaaaa ggtaatggag
     8821 ttatactaga aggagtaaca gattttgacc cagtacatat atttgagtgt ggtcaatgtt
     8881 ttagatggca caaacaagaa gatggctcat acactggagt tgcaaagggg agaatattaa
     8941 atgtcaaaaa agagaatgat aaaatatatt taaataatac taatttaaag gagtttaaca
     9001 gcatttggta taattacttt gatttaggaa ctgattatac agaaataaag aataagctaa
     9061 aaaatatgga tgaatattta aataaggcta ctgaatttgg atggggaata agaatattaa
     9121 gacaagatgg atgggaaatg cttatatcat ttataatttc ctctaataat aggattccta
     9181 tgatacaaag agctatagaa aatttatcaa gaaaatttgg taagtatata ggtgaatatg
     9241 aaggtaatga atactatgca ttcccaacac cagaagaact aaataaggcc tcacaagagg
     9301 aaataagagc atgtcaaaca ggttttagag ataagtacat aaaaagtact acacaggcag
     9361 ttatagagaa taatgatgag gtatctgaat atacaaattt aagtactgag gattgtagaa
     9421 aagagctttt aaaatttaat ggagtaggac caaaagtatg tgattgtatt gcattatttg
     9481 gtatgcaaaa atatgattct tttccagttg atgtttgggt taagagagtt atgcaagaat
     9541 tttatataga tgaagacatg agtttaccta aaatgagaac ttatggaatc gataaattta
     9601 aagaaatgtc tggatttgct caacagtact tattttacta tgcaagagaa ctcggtatag
     9661 gaaaataaat taagggggag aaattatggg agtaatagga acaatatttc tgttctattt
     9721 aattatatca tatctagctg gagccataat ttctgtgatt atattacttg aaaatagaga
     9781 ccctgcaaaa acaatgtctt ggttattgat gtttattata tttccaggtg taggtttaat
     9841 gatatatgct atttcaggta gaaatattag aaaaagaaaa ttatttaaga ctcaaaaact
     9901 agctaataat ataaaagaaa agaaactatt tgatacatta gaaaagataa cagaaatagt
     9961 tgaattagaa aaagaatcta taaaacaaaa taagctttta agagatgaag aggatggtag
    10021 ttatagaaaa agggtaataa atatgttatt aaagactggt atgtttccct ttactaaaaa
    10081 taataaagtg gatgtgtttg tagatggaaa tgaaaagttt aaaagactta tagaagatat
    10141 aagagaagct aaagaccata ttcatttaga gtactttatc atcaaagata gtgaaatagg
    10201 taggttgtta aaagaagaac ttataaaaaa ggctaaagaa ggcattaaga taagaatttt
    10261 atatgatgat gtaggatgct ggagattttg gtttaataga aaatttttcc gagaaatgag
    10321 ggaagtaggt atagaaattg cagcattttt acctacaaaa tttcctataa taggagggaa
    10381 attaaactat agaaatcaca gaaaaattgt tgttattgat ggaataattg gatatacagg
    10441 tggaataaat ataggagatg aatatctagg caaaaatgat aagtttggat attggagaga
    10501 tactcatatt aggattaagg gtatttctgt atacatgctt caaatgacat ttctgattga
    10561 ttggtattat acaacaaaag aagtattagt aactaaaaat tattttccaa gtgtagggaa
    10621 tattggtgaa agtatgatac aagttgttgc gagtggtcca gacagtgatt gggaagatat
    10681 tcattatgct tatttttctg caatatgtca ggctagaaaa aatgtttata tagaaactcc
    10741 atattttatt ccagatgaaa gtctactaaa agctataaag agtgctgctc ttagtggagt
    10801 tgatgtgaga ataatatttc ctaaaattgc agaccacaag atagtaaata tagcatctta
    10861 ttcatatttt gaagaaatat tgagagcagg tggtaaagtt tatttatata ataaaggatt
    10921 tatacattct aaagtcgtaa taatagatga taaaattgct tctgcaggta cagctaatat
    10981 ggatttaaga agttttatgc ttaactttga agtaaatgca tttatctatg atgaagaagt
    11041 tattagagta atgacagatg atttctttga ggatttaagt cactgtgaag aacttaattt
    11101 ggaggtattt aagaatagaa atatcataca aaaaatcaaa gaatctgtag caagattatt
    11161 ttcaccaata ttataaaaaa ttattaaaaa aggtattgaa aattatgtgt gattggtata
    11221 ttataataat tgttagcact ctattaggta gagtgataat aaataaaaag tatatatata
    11281 aatctacata tataagtagt attaatttaa aaataactaa aaacataggc gaactagtct
    11341 atacttaata ttttaggagg cgtgaataat gaaaataaga ccattagctg acagagtagt
    11401 aattaaaaaa gtagaagcag aagaaaaaac tgcaagtgga atagttttac caggagcagc
    11461 taaagagcaa cctcaaatag ctgaagttgt agaagttgga ccaggtggaa tagttgaagg
    11521 gaaagaaata aaaatggaat tgacaatagg agataaggtt atattccaaa aatattctgg
    11581 aacagaagtt aagatagaag gacaagaata cacaatacta agacagagtg atgtattagc
    11641 tgttattgaa taaatataga ataaatttat taggaggggt ttaaaatggc taaagaaatt
    11701 aaattttcag aggaaacaag aagagcttta gaagctggtg taaataaatt agcagataca
    11761 gtaaaagtaa cattaggacc taaaggaaga aatgttatat tagataaaaa atttggttct
    11821 ccacttataa ctaatgatgg agtaactata gcaaaagaga tagagttaga agatagattt
    11881 gaaaatatgg gtgctcaatt agttaaagaa gtagcaacta aaactaatga tgtagctgga
    11941 gatggtacta caactgctac agttttagca caagctataa taagagaagg tttaaagaat
    12001 gtaacagcag gggctaaccc aatactttta agaaaaggaa tacaaaaagc agtaacagta
    12061 gcagtagaag aattaaaaaa tcaatcaaga atagtagaaa cacaagaggc tatatctcaa
    12121 gttgcttcta tatctgctgg tgatgaagaa gttggaaaat taatagcaga agctatggaa
    12181 atcgtaggta aagatggagt tataactgtt gaagagtctc aaactatgaa tactgaatta
    12241 gatgctgttg aaggtatgca gtttgataga ggatttgttt ctgcatatat ggttacagat
    12301 gtagataaaa tggaagcagt tttaaatgac ccatatatat taattactga taaaaaaata
    12361 tctaacatac aagaattatt accagttctt gaacaaatag ttcaacaagg taaaaagtta
    12421 ttgataatag ctgaagatgt agaaggtgaa gcattatcta cattagtagt aaataaatta
    12481 agaggaacat ttgatgtagt agcagttaaa gctccaggct ttggagatag aagaaaagaa
    12541 atgcttcaag atatagcaat acttacaggt gctcaagtaa tatcagaaga attaggttat
    12601 gatttaaaag aagctgattt atctatgtta ggtagagctt catctgttaa ggtaactaaa
    12661 gaaaatacta caatagtaga tggttctgga gataaaaaat ctatagaaga tagagtaact
    12721 caaattaaac atcaagtaga gcaaactact tcagattttg atagagaaaa attaatggaa
    12781 agattagcta aacttgctgg aggagtagct gttgtaaaag ttggagctgc tacagaagta
    12841 gagttaaaag aaagaaagtt aagaatagaa gatgccctta atgcaactag agcagctgta
    12901 gaagaaggaa tagtagctgg tggaggaact gcttttgtta gtgttatacc agcaatagga
    12961 acactaattg agagtttaga aggcgaagtt aaactaggtg ctcaaatagt taaaaaagcg
    13021 ttagaagagc cattaagaca aatagctata aatgctggtc ttgaaggtgc tgtaattgtg
    13081 caaaatgttg ttaattctga agcagaaact ggatttgacg cattgaatga aaaatatgta
    13141 aatatgatag aagctggtat agttgaccca actaaagtta gtagaagtgc tttacaaaat
    13201 gctgcatcaa tagcaagtac tttcttaact actgaagctg ctgttgctga ccttcctgaa
    13261 aaagaagatg caggaatgcc aggaatgggt ggcggaatgc caggtatgat gtaatttaaa
    13321 aaatttaaaa tataaatttt aatataaaga actatttcga cttagatttt aaactctaag
    13381 ttgaaatagt tcttttgtat atatctaatt tttgtatatt tataaattca atacacatat
    13441 ttagataatc agtatctagt ttgtaataat attttagagt agtattgtta ttttttaggt
    13501 tatgatttta gatatatatt aattaaggta taattgttga tagagaataa atcaaattta
    13561 aaaggatgcg ataatatgga cttaagaaaa ttgcttgaag aagttaaaaa tgaaaatatt
    13621 gatatagatt ttgcgttaga aaagttaaaa gatttacctt atgaagacat tggatatgca
    13681 aatatagacc atcatagaga attaagaaat gggtatccgg aagttattta ttgtgaaggt
    13741 aagagtgatg agcatatttt agggattatt gataaaatga atcaaaaaag ttctaacata
    13801 ttggggacaa ggtgtagaaa agaaactttt ctgaaaatta aagaaatcta taatcatgct
    13861 gagtacgaag agttgtctaa aattcttaag attcaaaatc atgatataga gaatatagga
    13921 aaaggtaaaa ttgtgattgc tacaggggga acttctgata taccagtagc agacgaagcc
    13981 taccatacag ctaagttttt aggcaatgat gttgatagaa tatatgatgt tggagtggca
    14041 ggtatacata ggcttttaaa taaaagacat aaaatagata gtgctagagt aatagtagcc
    14101 gttgcaggta tggaaggtgc tcttgcaagt gtaattggtg gactggttga tgttccagta
    14161 atagctgttc caacttctgt tggatatgga gcaaattttg gaggattagc agcattacta
    14221 gctatgttaa atagctgtgc atctggaata tctgttgtaa atattgataa tggatttgga
    14281 gcaggatatt tagcagcaat gataaataaa ttgtaaatac attgatataa attaaacaaa
    14341 actatataat aaaatttaat ttctattata tttttactat taaaattgag gagacatata
    14401 tgagtgaaaa aatactttat tttgatataa taagcggtat atcaggggat atgacccttg
    14461 caagtttatt aaatttaggt gtgcctaagg aaattttttt ggaggaaatt cataaactaa
    14521 gaatgagcga tgaatttaat ataaacatca gctcgaaaac tgaaaatgga atagtaggta
    14581 caaaagtaga agtaattaca aaagaaaaac atacgcatag aaatttagtg gatatatttg
    14641 aggttataga taaaagcact ctaaacgaaa atgtaaaatt taaagcaaaa aaaatattta
    14701 tggtaattgg agaagcagaa gcaaaagttc atggaactac aatagacaag atacattttc
    14761 atgaggtagg ggctatagat tcaattgtag atatagtagg agcttctata ttagttgact
    14821 tactttgtgt agataaagtt tgtgctagta cagtgtctgt tggttcagga tttgtaaaat
    14881 gtgaacatgg aattatacct atacctgcac ctgcaactat agaaatttta aaaggtgtgc
    14941 caattaaatt aaataatgta aatggagaat gtacaactcc aacaggagct gcaatcataa
    15001 aagtcttgtg tgatgagttt gtagatgagt ttgaattttc tccaaagcaa attggatatg
    15061 gtataggtca taagaagttt gaagtgccaa atatactcag aactgtatta ggtgaaaaaa
    15121 aaaagaagaa ttaatttatg aaattagtgc aaacattgat gatatgtctt cagaaatcta
    15181 ttcttatttg tatgaaaaaa tattagatga gaaagcatta gatatttata cagagagtat
    15241 ttatatgaag aagaatagac cagcaacaaa agtatcaatt ttatgtaatg aatcagattt
    15301 agaaaaattt attaaaatat tacttttaga aactagtact tttggagtga gatataatgc
    15361 atataataga gaaattctag agagaaaatt tgtaaaatta gatacaaaat atggagtggt
    15421 tacagtaaaa ctagggtatt ataaaggaaa actagtcaag gctactccag aatataatga
    15481 atgtaaaact atagcaaaaa atgaagatat tcctataaaa aaagtgtttg ctgatataaa
    15541 ttttagaata aaagaagaat ttagtgaaaa cttattgaca taagtaataa attttgataa
    15601 attaatattc agccttatta aataaaagta aacagaaaaa aatatataaa atataaactc
    15661 actcgtatat gctcagaaat atggtctgag agtctctacc aagataccgt aaatatcttg
    15721 actatgagtg aaattattat accaaaagac ctagttaagt aaatgtgtag gctgagttaa
    15781 actccctatg tgttggctat aagataaagg tattttggaa gcctgtatag taatttcact
    15841 tttgctagaa attatggaag ttactataca ggttttttgt gtaaaaagat ttaatataat
    15901 ttagactgta ttgtgttaaa ataaatatat aaaaactata ttatacaagg agagatgtaa
    15961 atttatgaaa catgaattag tacttgtaat agattttgga ggacaatata atcagttgat
    16021 agctagaaga gttagagaaa acaacgtata ttgtgaaata cttccatgta ctgcaagcat
    16081 agaaagaata aaagaaaaaa atcctaaagg gataatattt acaggcggtc caaatagtgc
    16141 gtatttagaa gattctccaa caatatcaaa agaaatattt gaactaggag taccaatact
    16201 tggaatatgc tatggtattc aaataatgtc tcatgtattg ggtggagttg taagaaaagg
    16261 taataaacaa gaaaaagaat atggaaaaac agctataact tatggaaaat catcattatt
    16321 tgaaggaata actacaaata gtgtatggat gagtcataca gatttaatag aaaaagttcc
    16381 agaaggattt actatagttg caaatactaa tgattgtcca gtagcagcta tggaaaatgt
    16441 agaacgaaac ctatatggag ttcaattcca tccagaggta gaacactgtt tggaagggga
    16501 caagatactt acaaacttct tatataatat ttgtaaagta aaaggtgact ggactacaga
    16561 ttcattcata gaagataaaa taaaagaatt aaaagaaaaa ataggcgata aaaaagcttt
    16621 atgtgcttta agtggaggag ttgattcttc tgttgctgca gttcttattc ataaagcaat
    16681 tggggacaac ttaacttgta tatttgttga ccatggttta cttagaaaaa atgaaggaaa
    16741 tgatgtagaa agaatattta gagagaaatt tgatataaat ttaataagag ttaatgcaga
    16801 agatagattc ttatctaaat taaaaggagt ttctgaacca gaagctaaga gaaaaataat
    16861 aggtgaagaa tttataagag tatttgaaga agaatcaaat aaattaggaa aaatggattt
    16921 cttagtacaa ggaacaatct atccagatgt tatagaaagt ggacatggaa atgctgctac
    16981 tataaagtct catcataatg ttggtggaat cccagaagat gtagacttcc aagaaattgt
    17041 agaaccatta agagagttat ttaaggatga agttagaaaa ataggattag agcttggtat
    17101 agaagaagga ttaatattta gacatccttt cccaggacca ggtctaggaa taagagttat
    17161 aggtgatgta actaaagaaa aatgtgatat attaagagaa gctgatgctg tttatatgga
    17221 cgagcttaga aaagcaggtt tatacagaga aatatggcaa gcttttgcaa cattaccaga
    17281 tgttaagact gttggagtta tgggtgatga aagaacatat gcttatttag ttggtttaag
    17341 agcagttact tcttctgatg gaatgacttc tgactggtat aagatgcctt atgatgtatt
    17401 agaaagaatt tctaatagaa taataaatga agttgatgga gtaaatagag tagtttatga
    17461 tataacttct aagcctcctg gaactattga atgggaatag taaataaaaa agtcgtaagc
    17521 cttgtatttt caaggtttgc gacttttaaa aaaatctttg tgatactaaa atgatactat
    17581 atatttaaat tatctagttt ttgggcaact tcaagttgct tatttgggta taaatgtgaa
    17641 tatgtattcc aagttgtttc tactttttca tgccccaatc tttctgctat agttaatata
    17701 tttacatcca tatttaccaa taatgacgca tgagaatgtc tcaaatcatg cactctaatt
    17761 ctttttacat tagataactt acagcatcta tctagctctt tagaaagata acttttagca
    17821 aatttaaata ttctttcatt atcttttaag tcatatagct tagatagata atcttttata
    17881 ttattatata agaaatgtgg gatagggaca acacgtttac ttttaggagt tttaggagaa
    17941 gaaacaatat cctctccatt tagtcttatg tagctttttt cgacgcttat tttattttcg
    18001 aaaatatctt ttggagtaag agctaacaac tcccctagtc taagacctgt ccaaaataaa
    18061 atttcaaatg ctagtctagg ttctgatttc ttttcaaatt caataaattt tttaaactct
    18121 tctaatgtcc aaaagttcat ttcatctgcg ttttttttac cgatagaacc tgccaaatga
    18181 gcaggattag aaggcaagtt ataatattta actgcataat ttaatacagc aacaagctgg
    18241 ttgtttatcg ttttaatata tgtttggcta taatcacttt ttaataattc gttttgccat
    18301 cttcttatat gtgttgcagt aatttcattt atttttagac ttttaaaaaa aggtaaaatt
    18361 ctaaagttta ttaaatactt tttagtttct aatgtagata attttaatct agaagacata
    18421 tcgtgcatgt attcttctat gagactttca aaactcatat cagtactcat tttagattta
    18481 ttcaaaaatt ctctttcaaa ctctagagct tcttttttag ttttaaaacc tctcttaatt
    18541 ttctttttcc tatccccatc gaagtctgta aaataaaagc tagcatacca actttttctt
    18601 tgttcgtctt tgtaagcagg cattttagac acccccaatt atgtcatttt aatattacaa
    18661 taattataac atgttagata tgctatctaa ataattgttt tattttcctc ttctttttat
    18721 ttcttgaagc ttgtaatatt tacacagttc taagaagtct tcttctagtg ctatagataa
    18781 gtctaatatc gtagatatac ctatatcttt gtactcttga ttttctagtt ttgatatata
    18841 acttctgtta cgtctcattt tttctgctaa ttctagttgt gtcattcttt tcttttttct
    18901 taattttttc aacatttatt aaaatccacc ttttaattat ttttatgaca tttttataat
    18961 taatagtttg tgtaatttct tgtaaaaaat gttcctcatt agaacatttt ttggtggaaa
    19021 aatgtgttaa aatgtaagta agaaaagcac tttcaaagca ctataaaaat agtgctgaaa
    19081 tagataaaca aataaaagta ataaggagga aagatagatt ctagagggaa atcattatca
    19141 tttttaagaa cgtatgttca gttggtggga tagaaaaata ctttacaggg gatggtttat
    19201 ttgaatgaga atgaaaatat gagtttatat gtcagtaagt taaaagaagt attgaaaaat
    19261 aattcagaag agtataaaaa aataagagct aaaataaatg aaatttataa tctaaatgaa
    19321 aagaaagaat aaaagggctt tagctctttt attctttctt agtattaact gctttataat
    19381 aagcattatc tatcattttt tttattgcat ttttatcatc tgaatctaaa gatgctaatc
    19441 tatttattag ttcgttaact tcattatctt cttttgagtt aacttttttt aataattcat
    19501 catcttgttg agtgttaggt tgtattttac ctaatttagt taatagctcc tttaaagtta
    19561 aatttaatcc aaaactcatt ttttcaagca tatctaaagt aggttcaaca gctttaccat
    19621 tccttgggtc tctgtttttt tctatcttat ctatatatgt atggctcaca ccacaaagat
    19681 tagaaaattc tctaagagat aagttatgtt cacttctata ttctttaata atttctgcta
    19741 atgtttgcat aatttcacac ctcacctaaa taatagttga cttaattata tcagaaaaat
    19801 gtaaactatt gttaacaaaa ataaaaattt agtaaaaaat aattgacaag agtaaaaaaa
    19861 agacgtatac tatgattaac aaaaaggtta taaataaagt taggagggat taatataaaa
    19921 aataatttac aatatattag aaaagaatct agaatatcac aaaaaaaatt cgctgataag
    19981 ataggtatat caagaccgta tttatctaga atagaaaacg gtaaggttaa tccgagttta
    20041 gaaatagcgc ataaaatctc gctcgaaaca gggaaaacaa taaatgaaat tttttttgat
    20101 tttactgtaa atcatagtaa actataaggt tttaattgta ccttgaaaac taaatataaa
    20161 atattttaaa ggagcaagta tatgaaaaat aaagaaaaag aaataatcat tagaggaatt
    20221 ttaataggaa ttttttattt tttaggaatt gtatttagta atactttttt taaataataa
    20281 atagaagggg taggtgaaat accaactccc caaatcaaaa taacactttg gaagttaaat
    20341 atagaatatt caaaagaggt gattagatgg aaatagagca aacaacaata cgcctgctaa
    20401 aaagcagacg aaagttgaat attaatttct attttggtta ggcgaaagac ctaacaaaaa
    20461 atctgtagat acattaagtt tgttagaaat taatattaaa gtttctatat ttggctctct
    20521 tgaacccgat tcataatatt gatatgcacg ttctgagata ccaaacaaac tagcaaattg
    20581 cttttgagtc atattcattt gctttcttac tagcttaata ttatctttga atttagtcat
    20641 aactacacgc tccaataaaa aaataaataa atttcttgac acgaacaata ttagcgtgta
    20701 atatataaaa catagcacga acaatattgg cgtgttaaat aaaaaggaga gggtgaaaat
    20761 aaataacaat ttaaaactac aacgtgaaaa agttggctta acgcaattag aggttgctca
    20821 aaaagccaaa ataacagaga gaagctatca atattatgaa gctggcgaac gtctcccaaa
    20881 cattcgtaca gctttaaaga tagctagaat tttaaatact aattgtgaaa aactctttaa
    20941 tgaataatag cataaaagga ctcagattac aagaaaggag ctttagatat gaataatttg
    21001 caacttaaca ataaaaatac aataacaaca ttagaaattg ctgatatgtt agaaattaac
    21061 cattgggaag tattgagaaa attagaaggc acagaaaaaa caaaaggaat tattgatatt
    21121 cttaacgaca acaattttgt tgtggttgat tactttataa aatcagctta tttagattta
    21181 aaaaatgaaa atagaaaatg ttacaatgta acgaaattag gctgtgactt tttagcaaat
    21241 aaatttactg gagaaaaagg aattattttt acagctagat atgtaaaaag atttaataaa
    21301 atggagcaag agttaaaaga acaacaacct aaactaccaa ctacatacaa agaagcatta
    21361 caacagttat taatagaagt tgaagaaaaa gaacaattac aattagaaaa tcaagaaaaa
    21421 gataaggtaa ttcagttaca gcaaccaaaa gtattatttg ctgattcggt agcgtcttct
    21481 gacaattcaa tcctagttgg agaattagca aagttgctta gacagaatgg aattgataca
    21541 ggacaaaata gattatttga ctggttaaga aataatggtt acttaataaa acgtaaaggt
    21601 gaggattaca atacaccaac tcaaaaaagt gtagatttag gagttataga aacaaaagaa
    21661 ggtacaagag tacatccaga tggtcataca agtattacta aaacacctaa gattactggt
    21721 aagggacaaa tatactttat taataagttt aaaaagaaca atcaaatatc aatgttaggt
    21781 taaagattaa tagcactttg aaaactaaat acagaatatt ttgaaatata ttgttttaat
    21841 taattattaa ctaggaggtt aaatacatga aaaataatac aagcgattca agagtaaaat
    21901 atttttgcaa gtgtccatat tgtgggtttg ataatgaggt agaagttaaa aaagggtcga
    21961 agcctaaaat atgttgtata tgtacaaaag aagttgagta tgaaaaattg gagcaacaaa
    22021 gtgttttgga aaataccgaa attaaagaag tatgtaatca aatgaaaatt aaaaaattag
    22081 cactagcaac aggagaagta tttgaaaata tagagctcaa agaatttgaa aataaaggag
    22141 aatgcttacc accaaatatg gtaaaggtta ttgatggtaa gcaagaactt ctaataaata
    22201 aagagttcat attatcatta gaagtcaaaa gaaagactac tttttaggac ctttcttagt
    22261 ttgtgacaaa gcactaccag caaccgattt ggaagcagca ctatatcttc cgtctttaag
    22321 aatcttacta gccttagaag caacagtttt acttgtttgt ttagtatttt tagctatagg
    22381 tatcaccacc tttaaatgta tttataggat ttatcctaca aatatagtat atcaaaggag
    22441 ggaaataatg gcaattaatg acaacataaa taaaatttta agagatagag atttaaaagc
    22501 gtggaaatta gcaaaagaaa taggcgtaga ttcaggaaat ttatatgcaa ttttaagagg
    22561 agaaaataaa aatccaacta tagatacatt gataaaacta gctgactatt tagatgttac
    22621 attagacgaa ttagttggaa gatagaaatt aaatacagaa tattttgaaa gtaggtgtat
    22681 tgtatggcaa aagcagtagc taaagaacaa ttgttttata gggcaaaaga tatagctaag
    22741 attttagatg tatgtgaagc aaccgcttat aaaataattg gagaattaaa taaagaatta
    22801 gaaaaagaag gttggaagac ctttcctgga agggtatcag taacttattt caaagaaaga
    22861 tattgttata aaacaaaaaa aggggcataa aaaatgctat gagagtagct aaagatatgt
    22921 ttcctaaagt aattgtataa agaggaaggg ggtgatttag ttgaatgtaa gagtactgat
    22981 agcttatata cagttttgta agcaatacaa taagaaagcg agttttgaag gtcttaaaaa
    23041 atacaataag gggatagtga tataaaaaaa cttaaagtaa aatttcctac aaaaattctt
    23101 aaagcaggta aagcaatcag agtaacatgt gcaagatttg gttttgaaag cgattgtata
    23161 ataactcaat cgcaagaatt tgaattaaca gtagtttatt ttgataaaga attagatgat
    23221 ttagtacaat cgtctataac tatagatgat gcaataagct atgattattg cattgaaacg
    23281 ttaaattaag aggggattaa tcatgaaaag tttaattata gtgagaaatt cagtagagca
    23341 acaactaaat agagctaatt tagaaataaa taaaaacgag caactttata caaaacttag
    23401 aaaaaaagaa gaaagaaata tatcagaaga aattgaattg agtaatgctt taagagaaaa
    23461 aagtgtaaac gaaagattaa aaatatttgc tgagtcatta ctaaaaatta tagatacaca
    23521 aattgaaata aaagaatatg aagaaagcga ggattacaag atatttgaat taatttcaga
    23581 agaacttgaa agagatatac ctatagatgt tcagatataa gaaaagagcc actgcaatgg
    23641 ctctaatcaa aaatatatca aaaatttaat tagctatatt atagcataaa tggagggaaa
    23701 ttatgagtac tttatacgaa ttaactacag atttattaga aatagaagaa ggtttaacag
    23761 aaacaacagg aaatgaagct gaaaaactag aggaaataaa agaaataata aaacaagaga
    23821 tacaaaataa aaacactagg atagtttcag taatattaaa cattgacagt gatataaact
    23881 ctatagattc agagattaaa agattgcaag agttaaaaag ggccaaaaag aatactcttg
    23941 ataaattaaa aagcaatata aaagactgta tggaattact tgagactaaa aaagtagaaa
    24001 caattttagg aaatataagt ataagaaagt cggcaggtag cttagtcata gaagatgaag
    24061 aaaagatacc tgctatatat aaaacagttg agcaagttgt aaaagtagat aagaacacta
    24121 ttaaagactt tattaaaaaa ggtcatgagg ttgaaggttg taggattgaa tatggaacta
    24181 cactaacaat tccaaaagct aaaaaagagt aggtgaggac catggaaact aataatgttt
    24241 atataaaact tgtaaatata cagagtactt taaaagctcc taaaagtcaa tttaatagct
    24301 ttggtaaata caactatagg agttgtgagg atatactaga aggtttaaag cctattctaa
    24361 aagaagaaaa agcattggtt gtattggatg ataatattgt tcagatagga aatagatttt
    24421 atgtagaagc tacagcaact ttaatagatg cagaaacagg agaaaaaata tctgtaaaag
    24481 cattagctag agaagatgaa actaaaaaag gtatggattt agcacaagta actggaagtg
    24541 tatcaagtta tgcaagaaag tatgctttaa atggattatt ttgtattgat gatacaaaag
    24601 atagtgatgc aacaaataaa catggaaatg agcagaaaaa aaaagaagtt aatgagagcg
    24661 aattaaatat actatattcg ctaggagaat ctatagaaaa agataaaaat agagttgata
    24721 gtgaagtata taagaagttt ggaaagttag cagtagattt gactaagcag gagtatgaga
    24781 aagttttaaa tggatataag agcattttag agaagcaaaa acaagagtag gtgatagtat
    24841 tggggattat aagagtaagc aaagacaaag ataatccata tgtagtttta aataaaactt
    24901 gtttggaaga tgtaaaatta agctggcaag caaaaggctt acattcatat ctgattagta
    24961 agcccgacca ttggaaaatc tatgttaatg acctatataa aagaagtaaa aatggaaggg
    25021 atgctacagc aaatatttta aaagaactta tagaaaatgg atatgtaaca agaaaacctt
    25081 gtagagattc taatactaat aagatgcttg gaggatatga ttatcaagta tatgagatac
    25141 cacttgaaaa tcctcagaag ctaaaatccc gaaaaactga tttcccggaa accggatttc
    25201 ccggaaaccg ggtttctcgg aaaccggaaa acacggaagt agtaagtaat gactttaaag
    25261 taaataatga tattactact attgttatta atgaacaatc caataaagat aaaaccacct
    25321 acataaaaaa atattatgaa tcttatatag gtgtgattac tccaaataat tttcttcaac
    25381 tactaagtta tttagatgat ggaatggaag ctgatgtaat aataagagct gttgatgaag
    25441 ctgtaggcag tggagttaag aattataaat acattaaaac aatattaaat aattggatag
    25501 aagcaggtgt aaaaactaat ttagaactta cagagtatca aaatgagttt gagaggaaga
    25561 aaaagagtaa acaggagaag aaacagtcta ataataaaac tgtgaatact cataatgtaa
    25621 gtaaaaataa gtttgctaac ttcaatcaga cttttactca gtatgatgaa aaagagctag
    25681 acgagattat taaaaagagt cagaaggaaa aatttaaata aaattaaact tctaggaagt
    25741 aaatatcaat atattgcttc ctagaaaagg ggaggtataa aatggcgaga atatatgcac
    25801 aaagaagtgg ttctttaaat gaacaagata gattggagtt attaagatta cttgggaaag
    25861 ctggatatac agtaaaggtt gctagagaga agcaaaatag caagacaact tatacttact
    25921 ttgttgagta tacagaaga
//
LOCUS       DAELJO010000033        25470 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELJO010000033 DAELJO010000000
VERSION     DAELJO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.033"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            281..397
                     /locus_tag="KPB88_001243"
     CDS             281..397
                     /locus_tag="KPB88_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG0053540.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            561..1628
                     /locus_tag="KPB88_001244"
     CDS             561..1628
                     /locus_tag="KPB88_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0053541.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEAAKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1820..2350
                     /gene="rnmV"
                     /locus_tag="KPB88_001245"
     CDS             1820..2350
                     /gene="rnmV"
                     /locus_tag="KPB88_001245"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG0053542.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRYEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2521..3390
                     /gene="rsmA"
                     /locus_tag="KPB88_001246"
     CDS             2521..3390
                     /gene="rsmA"
                     /locus_tag="KPB88_001246"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG0053543.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3456..4721
                     /locus_tag="KPB88_001247"
     CDS             3456..4721
                     /locus_tag="KPB88_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053544.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4872..5987
                     /locus_tag="KPB88_001248"
     CDS             4872..5987
                     /locus_tag="KPB88_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0053545.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6173..7069
                     /locus_tag="KPB88_001249"
     CDS             6173..7069
                     /locus_tag="KPB88_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0053546.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7139..7936
                     /locus_tag="KPB88_001250"
     CDS             7139..7936
                     /locus_tag="KPB88_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053547.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7992..9344)
                     /locus_tag="KPB88_001251"
     CDS             complement(7992..9344)
                     /locus_tag="KPB88_001251"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG0053548.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9563..10441
                     /gene="purR"
                     /locus_tag="KPB88_001252"
     CDS             9563..10441
                     /gene="purR"
                     /locus_tag="KPB88_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG0053549.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10597..10875
                     /gene="spoVG"
                     /locus_tag="KPB88_001253"
     CDS             10597..10875
                     /gene="spoVG"
                     /locus_tag="KPB88_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG0053550.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11088..12467
                     /gene="glmU"
                     /locus_tag="KPB88_001254"
     CDS             11088..12467
                     /gene="glmU"
                     /locus_tag="KPB88_001254"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG0053551.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEHINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12562..13512
                     /locus_tag="KPB88_001255"
     CDS             12562..13512
                     /locus_tag="KPB88_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG0053552.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14350..14435
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14597..15109
                     /locus_tag="KPB88_001256"
     CDS             14597..15109
                     /locus_tag="KPB88_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0053553.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSIKSAALSYYSDTNKMPATTSNPVDLENLKTYMESLPDKADIGGEYQLLLVG
                     NKLVLQINDATLTGAQSTKLLSDLGNDKIYKTIGSDDKLTDLLTTNEKLDNKVLYLVL
                     IDNAEMDSTK"
     gene            15261..16937
                     /locus_tag="KPB88_001257"
     CDS             15261..16937
                     /locus_tag="KPB88_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0053554.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16949..18157
                     /locus_tag="KPB88_001258"
     CDS             16949..18157
                     /locus_tag="KPB88_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0053555.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18172..19884
                     /locus_tag="KPB88_001259"
     CDS             18172..19884
                     /locus_tag="KPB88_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG0053556.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19877..20785
                     /gene="pilO"
                     /locus_tag="KPB88_001260"
     CDS             19877..20785
                     /gene="pilO"
                     /locus_tag="KPB88_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG0053557.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20812..21378
                     /locus_tag="KPB88_001261"
     CDS             20812..21378
                     /locus_tag="KPB88_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0053558.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21415..21942
                     /locus_tag="KPB88_001262"
     CDS             21415..21942
                     /locus_tag="KPB88_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0053559.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21963..23501
                     /locus_tag="KPB88_001263"
     CDS             21963..23501
                     /locus_tag="KPB88_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053560.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSINKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILEEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIINFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23601..24683
                     /locus_tag="KPB88_001264"
     CDS             23601..24683
                     /locus_tag="KPB88_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG0053561.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24664..25446
                     /locus_tag="KPB88_001265"
     CDS             24664..25446
                     /locus_tag="KPB88_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0053562.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
BASE COUNT        10393 a         2669 c         4614 g         7794 t
ORIGIN      
        1 attttaattt cattattaaa gtatagatgt tatatataaa gtaaataaga tattaaataa
       61 ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata tatataattt
      121 aatttgagca gtttgttata tattataaaa aagagaaaaa taaaaggtaa attgtttctg
      181 gataacttaa aattggaagt aagaatattt aactagagaa gaattattta agctcaggtt
      241 agctctagga gtagctgatt ttataggagc aatagatttt atggatgatg atataaacat
      301 gatagcaatt agaccagaag atataagggt agaatttaat agagatttca aagaaattat
      361 agacctcaaa ctcagaccgt atcgatataa aaactaaagc tagatgatat agtgttgtat
      421 ataggaccat cagctgttat ggcagtatta gtgcttttaa caaatataat agtaaaactt
      481 ttgtatgaat tgataataaa aaaattaaaa accaagacat aaaaatggca acaaaaagaa
      541 gttgaataag ggaggtaaaa atgaaattta agaagttagt ctctttggga atattatcaa
      601 cacttttatt gagtgtaata actggatgta gtaatgtttc atcaggttca aacttaaagt
      661 ctaataataa gacaaaagga gagttaacag tttatacagc aattgaggaa gatagtatag
      721 atacatactt agctacattt aaagagaaat atccagatat taaattgaat gtacttagat
      781 tatcagtagg aggtataaca gcaagattat tagcagaaaa agataatcct aaagcagatg
      841 tgttatgggg tgttgcagct acaagtttga taattttaga tgacaaagaa atgctagagc
      901 catacgctcc gaaaggcttt gataaggtaa atcctgcatt taaagatgat aagaaagttc
      961 cttcttgggt tggaatagat gcttggatga ctggtataac agtaaatact aaagaattaa
     1021 aagctaaaaa tatatcagct cctagttctt atgaagattt aataaagcca gaatataaag
     1081 atcttatatc tatgcctagt ccagcttctt caggaacagg atatttaaca gtatcagaac
     1141 tcatacaaat tatgggtgaa gaaaaagcat ggcagtacat ggataaatta cataaaaata
     1201 ttggtgtata cactcagtct ggttcagcgc cagctatatt agcagcttca ggagaatatc
     1261 ccattggtat atcttttgga aatagaggta taaagttaaa aggtgaaggt tatccagtag
     1321 aagtagtatt tccaaaagaa ggttctggat gggagataga atcaaatgca cttgtgaaaa
     1381 aagataatat aaaagaagca gcaaaagtat ttttagattg ggctataagt aaagatgcaa
     1441 tgaatgagta ttctaagaat tttgcagtta ctacaattga cacgggaaat ccggtgccag
     1501 aaggatttcc aaagaatcca ttagaacaga tgatagataa tgatttaaaa tcagcagcaa
     1561 gaaatagaga ggctatacta aataaatgga tagaaaaata tgatggaaag acagaaaaag
     1621 agagctaagc cttacaataa aatttagtac aaaaagcgcc tagaatataa gttttatatc
     1681 taggctcttt tgatatattt atttaaaatg catatttcta tataaaaata aatagtttag
     1741 taccttgatt aaattaaata aaaaccttaa aataaagaga tgtaaagaaa aactaaaaac
     1801 gaataaaagg tgaatttata tgataaaaga aataatagta gtagagggta gagatgacgt
     1861 tacagcagtt aagagagcaa tagatgcaga acttataact acaggtggat ttggatttcc
     1921 aaaaggtgta atggagcgta taaaagctgc taatgaaaga cgtggtgtta taatatttac
     1981 agacccagat tttgcaggtg aaaaaataag aaaaaaaata gcatcagaag ttccaggatg
     2041 taaacatgca tttctaccga gagaggaagc taaaaaggat ggagatatag gtattgagaa
     2101 tgctactcct caaagtataa tagcagctct gaataaagtt agaactgaaa atacagaaaa
     2161 aagatatgaa tttaagcaag ttgacttaat tagaaatggt cttataggta atgaagaagc
     2221 atcacttaga agagatgcac ttggaaaaat acttggaatt ggatatggaa atgcaaaaca
     2281 atttttaaat agattgaata attatggagt tgaaagagaa gaatttatag atgcattaaa
     2341 acaaatctaa acgtaatgtt taatgtatga aaaatatgta tataaaataa attgtggaat
     2401 tacggttaat ttaagataaa ttatttataa tatatacctt attttaaatc aatgtaaata
     2461 catataaata agtataatta ataaaattaa atatcaaaat aagttaaaaa ggagtaaata
     2521 atggatagac tatcttcaca taatgcaaca aaggaagtag tacaaaaata taattttaaa
     2581 ttttcaaagt ctttaggtca aaatttcttg atagatagta atataattga taaaatctta
     2641 tctggagcta gaatcacaag aggagataat atcatagaag taggacctgg tataggaact
     2701 cttactcgtg agatgggtaa aatagcagaa aaagtagtgg ctatagaaat cgatagaaat
     2761 ttaattccta tattgaaaga taccttgtca gatttagata atacagaggt tgtaaatcaa
     2821 gatatactaa aagtagacat acaggaatta gtaaaagata aattaaatgg tgggccagtt
     2881 aagcttgtag caaatcttcc atattatata actactccaa tagttatgaa atttttagag
     2941 gaagacatac ctgtaacaga tatagtagtc atggttcaaa aagaagttgc agatagaatg
     3001 aatgctattc cgggtactaa agattatggg gcattatcta tagctgttca atactattgt
     3061 gatacagaga ttgtggcaaa agctccaaga catatgttta taccacaacc aaatgtagat
     3121 tccacagtta taggtctaca tgtaagagat aaaagaaaat atgatgttca taatgaagac
     3181 atatttttta aaacagtaaa agcatccttt ggacaaagaa gaaaaactct tcttaattcg
     3241 ttgggaggtc ttggttttct taataaagat gaaataagag aaatattaaa agaagctaat
     3301 atagacgaaa aaagaagggg tgaaactctt agtatagaag agttttcagt tctttcaaat
     3361 ataataaata caaaagtatc ttctaaatag aaggtacttt ttttaatttt gttaacatat
     3421 attgtttaga gaaactgtgt taagggggga ttgcagtggg ttctaaaaga aagtttaaaa
     3481 gagactatat aatactagaa gctaaagaca taaactttag atacaaagaa cgagtactgc
     3541 caaaagcttt tgcaaaagtt gaagtaaatg atgaaaaagc aattattgca ttatatgtag
     3601 aaaatctaaa atatgttaaa gatgggtata aagcagtagc aataaaagat gaccatggat
     3661 taatagactt aggaaatata attttaaatg aacaaggaag aggtgaattt gttttagatt
     3721 tagaagatta tgatgatgag ataaaaggta ttgcattatt gtatgaaaga aatatacctt
     3781 taattggatt taaaggaaat aagcttgaaa attatgagga catattattt caagaggtag
     3841 aggaagaaga atatgaagaa tatgaagagt tggaagaaga agacgaagag atagaggata
     3901 atgaggaaag tgatagagac gaaggtagtg aagaagaata tattatagaa gaatatgatg
     3961 aagatgagta tgaagactat tgggaagatg atgaagatat tgaagaagga gagttaaaag
     4021 caagaacgga agtagaagat tctagtgatg aggaaataga agagttcaat aattataagg
     4081 aaatagaaaa ttataatagg gttaaagtac aggaagaata tatagacata gaatatgatg
     4141 aggaagaaga ggaagaagaa gaagtttttg aagtaaatag agaagaagat aggtttgttt
     4201 cctcaaaacc tactaaatca aagaaagttc aaaaagatca gaataatatt gaaaaaataa
     4261 aaagagctaa tggagtaagt caaggagaga gtggaaataa acaaaccagt acattactta
     4321 tgccaagaca aataaaaaaa ggtttgaaat tatttaaaga agtaaaacct tttgtaacag
     4381 attatataga taagacaaga tggtggaaaa tagagataaa tcctacaaca ctgtgtggat
     4441 atacaatgcc ttatttagga tatttaaatg tattgaatta cactatgtat agtgatgcag
     4501 ttttacattc atataaatac agacactatt tatttggtgt tcagtatgat gaatataata
     4561 agagaaaaca ttatatttat gctatcccag gaaatagaaa tgaacagcca gacaatggaa
     4621 gcacaggatt tttaagatat caagcttgtg ataatagaac aaatagttta ggatattggc
     4681 tatgttttat agattctaga caaagaaata tagttaggta aaaataataa aaatgaaaaa
     4741 atttagactc aattcttatt ttactgaaaa aattaatgaa atgtagtaca aaactttaaa
     4801 aactattgat aatatttaaa tattacacta caatatatct tgaaatacct attatattat
     4861 ggaggaaaat tatgataaac gagcaaagaa ttttaaatga gtttcttgaa ttagtacaga
     4921 tagatagttt atctttaaaa gaggggaatg ttgctaaagt attagttaaa aaattagaag
     4981 aaataggttg ttcagtagta atagacaatg ctggtgaaaa agcaaatgga gaaactggta
     5041 atataatagc tacattaaaa ggaaataaag aaggaaagaa aatattattt agttcacata
     5101 tggatacagt aactccaggt ataggggtaa aacctatagt ggatgaagca aatggaataa
     5161 taaaaagtga tggtactaca atattaggtt ctgatgataa agcaggtata gcagctatat
     5221 tagaaggctt aagatatata aatgaaaata acatagaaca tactgatata caagttgtat
     5281 tctctatatg tgaggaatgt ggattagttg gagctaaaaa tttagactat ggaaaaatag
     5341 attctgaata tgcatttata ttagatagtg gtggttctcc aggtgaaata atagtaaaag
     5401 ctcctgcaca agatgtaata aatgttaaga tattaggaaa gactgctcat gcaggtcttg
     5461 aacctgaagc tggaataagt gctataatgg tagctgccag agcaatagaa aatatgaatt
     5521 tacttagaat agacgaagaa acaactgcta acataggaat tataaacggt ggaacagcta
     5581 caaatatagt aacaggagaa gttaatataa ttgcagaagc cagaagtcta aaagaaaata
     5641 aattagatgt tcaaactaaa cacatggtag agacttttga aaaagctgca aaagattttg
     5701 gagctcaaat tgagatagat gtcaatagag catatacacc aatagatgtt agtgaggatt
     5761 ctgaaataat aaaacttgca aagaaggctt tttcaaactt aggtatagaa ggtcatactg
     5821 aatctacagg aggaggtagt gatacaaata tattaagtaa gaatggtatc gaagctataa
     5881 ctttaggaat tgggatgaaa aatgctcata cactaagtga acatatagct ataaaagact
     5941 tatatgattc tgctaaaatg gtagtggaga taataaagga agcataaatt taaataatag
     6001 tgtataatat atatatcgcc tagcttaagc taggtgattt ttcattttat agaagattga
     6061 taatctctat tgaaagcaaa gagtatacat ttgcatataa gagagtgttt aatttatgtt
     6121 caaatgtagg ttcttaataa tatttagatt gtttcataag ggaggaataa atatggaaac
     6181 aaggtatgaa gaagcaaata aaataacaat acaatcaata ctttggaatg tagtattaac
     6241 aataattaag gttatagctg gtgttattgg taattctagc gctatgattg cggatggatt
     6301 acattcagca tcagatatta taagttctat aggagtacta ataggcaatt atgtatcttc
     6361 tagacctgga gatagagagc ataattatgg tcatgagaaa gcagagacat tagtatcatt
     6421 tgtgttatca atattgctta ttttcgtttc tataacaata ggtatagagg caattaaatc
     6481 tttatttaac ttagatgcat taagtgttcc atcaatatta ccattagtag tatcagttat
     6541 ttcaatcctg ataaaagagt atcaatatag aataacaata aaagttgcta aaaagataaa
     6601 ttcaccagcc ctaaaagctg atgcttggca tcataggtca gatgctttat cttcagtagc
     6661 tgcttttata ggtataggtg gttctatttt agggtttaaa ccattagacc caattgcgtc
     6721 agttgtagtg gctatatttg tagctaaagt aggaattagt atacttataa gttctgttaa
     6781 tgaattgatg gatgtttcag ttgatgaaga agagataaaa gaattaaaat ttattgtagc
     6841 agatacagaa ggtgttaaaa atcttggtga tataaagact agaaaacatg gagctatggc
     6901 atatgtagat ttgactatat gtgtggatga aaatttgaca gttaagcaag gtcatgatat
     6961 tgctaccaag cttgaaaaac atataataaa acatatggaa tttgtaaaag gcataacagt
     7021 tcacgttgaa ccatgtacta attgccaagg aaataagtgt aataattaat taaaacttaa
     7081 aaaaaagtgt aaaaagttat ataattgttt tatgattata aactctaggg gtggttaaat
     7141 gagcaagaaa agaaaactaa agaaaaaagc tgcaatatta ataggagtag ttgttttctt
     7201 tataatatac tttatatcct ttagaatagg aataaatatg gaaaaggata aaagtgaagt
     7261 gccaaagaag gtagaaaaag aagaagaaga taataggtct ttaaggactc aaataaaaga
     7321 gaagaagaag gtttatatat cagataaaaa tatagaaaac ataaaaattg aagaagatat
     7381 gtgggatgaa ataaagttat ttttctcaga ttttaagaaa gtaagaaata ctacttcttc
     7441 ttacgaagca gtttatgaag gttattctga tgatggagtt aaattttcta ctgaccttaa
     7501 cttttttaga gtatatactg taaataaaga agaatattat aaagtaccag ttagttctaa
     7561 ggatgctttt aagaaacttt tagatgagag catatatact tcgtttgacc ttttaagtca
     7621 ttataaagat tggaaagagg ttactataac tcatggaaaa gaaattaaaa aagttcatag
     7681 atggaaattt gatgatttag cacataagat gatttcaaag agggttgtag gaaaagttca
     7741 acctgagaaa agtaaggaaa gaagtgacta taattttact ataaatataa aaggtgataa
     7801 ttttgatgta aaagttgata caatgggaaa agattatgta aaagttactt ctaagaaaaa
     7861 tgtatcttac tatgaggttc atacaagttt atttgattac cttaaaaatg agatatttga
     7921 aattggaaag aaataagact ttaagaaggc tatcaaatta gatatttgat agcctaaatt
     7981 atttttatat attatttttc aaccaacaag tctgcaacct tataaattgg accagcacca
     8041 gcagttatta ctaaatcatt atccttaaca ttttcacgta agtattttac tatatcttca
     8101 aattctttta tatagatggc atctacattg ttttgatata atttctcaac caagtcctta
     8161 gaatgtatat ctccagggtc tttttcccta gcagcatata tatctgttat gataacttta
     8221 tcagcagcat agaatgcctc tgaaaattca cctaaaagag attttgttct agtataagta
     8281 tgtggctgga agatacacca taaagttgat ttttttaatt ttttggcagc agctaaagtt
     8341 gcttttaact cagttggatg atgtgcataa tcatctacta ctaaagcatt cttataatac
     8401 cctttagttt caaatcttct tccgacacct ttataaattt ttatattttt tcttatagtc
     8461 tctaagtcaa taccagaaac aagagctgcc attatagcag aagaagcatt gtatatgtta
     8521 tgaagaccat atacagaaag ttcaaattct cctaaatctc ttccctcata ttctattcta
     8581 aatattccat gaccactatt gtcaaagtga atatctttta ttacagcatc attgtcagaa
     8641 tctctaccgt attttataat agtagcttta acatcataca atatgtcatc aatattttca
     8701 tcatcgccat ttattatgaa atagccttct gggggtaata attttccaaa tttgttaaaa
     8761 gaagccttga tttcatctat tccagagaaa taatcaagat ggtcttcttc aacatttaaa
     8821 actatagata tctttgggtt aaaattaagg aaactatcta catattcaca tgcttcagtt
     8881 ataaagtggt tggaattacc tatcttaaca ttaccaccta tcatacttaa gttaccacca
     8941 actagtatag ttgggtctaa gtctgcatat tcaaagattg tagataacat agatgttgta
     9001 gaagtttttc catgtgttcc agaaactgct atagaatttt tatattccct catgatttgg
     9061 cctaaaaaag ttgctctgtt cattaccaat ttattttttt ctttagcagc cacaagttct
     9121 tcattatctg gatgaactgc tgctgtataa acaaccatat ttacatcatc tgatatatta
     9181 tcttttttct gaccaatata aatagttgcc ccttggtccc taagtttgtc taataggtat
     9241 gattcgtttg aatccgaacc tgaaacttga taacctttat tgatacatat ttcagctaat
     9301 gcactcatgc tgataccgcc aattcctata aagtgtatat tcataataaa accacctttc
     9361 tttttaatta agtcctacaa tatgaactat ttctgtggta taataatata atgttcaata
     9421 ttttataatt aatatgcata taatattata tgtattttat ttttgaatat aataaaacat
     9481 aagtaatatt ataccacaat aagaattgtt atgcattagt tagtttaacg ttatataaag
     9541 gtacttgtag gaggaaataa taatgaaatt taaaagaaca gagagaattg gagcaatagt
     9601 taaaatactg tctgataatc caaataaaat atatacttta agttatttta caaatcaatt
     9661 taatgcagca aaatcaacaa taagtgaaga tttattagtt gtaaaaaatg tatttgagaa
     9721 gctacattta ggtaaagtta taactatttc aggagcagca ggaggagtaa aatatatacc
     9781 taaaacttct atagcagaaa atgcagagtt cttaatggaa ttatgcgaga gaatatgtga
     9841 caagtctaga atactatctg gtggtttttt atacttgatt gacctaattt acgaccctac
     9901 aatagctgct aagataggaa agatattcgc atcaaatatt gagtatgtag atgcagatta
     9961 tgttgtgaca atggaaacaa aaggtatacc tatggcactt atgactgcaa aagctatgaa
    10021 tttacctcta gttataataa gaaaagatat aaaggtatca gaaggtccta cacttagcat
    10081 gacatatgtg agtggaaaca gttctaaagt tgaaagtatg agtttgccaa gaaaagctct
    10141 taaaccagat agtaaagtta taattataga tgatttcatg aggggtggag gtactataaa
    10201 aggaatggta gaccttatga atgaatttgg tgcagaggtt ataggtactg gagtattcat
    10261 atctacaacg aatccttcag aaaaaatggt aaaagattat atttcattga ttcaattgga
    10321 tgttaatgga gataaaatag tagtagaacc aaacttaaag acttttaaag atgaatatag
    10381 aaatgaagat ttagacgaag aagaagattc agaatttgaa tttgagatag atgaagatta
    10441 ataaaaaaaa tatttagaaa aatatgtaaa aataaaaaga ggatatccct agttgttcat
    10501 agaattattt aaaaaacagt ttatattaat ttattccagc gataataatt ttaatataaa
    10561 ttaatttctg tcaaaatcaa agggggatat tttggtatga agataactga cgtaagagta
    10621 agaaaattaa cagaagaagg taagatgaaa tgtatagttt caataacttt cgataatttg
    10681 tttgtagtgc atgatataaa agttatagaa ggccataatg ggctatttat agcaatgcct
    10741 agcagaaaag taggcgaagg aaatttcaga gatatagctc atccaataaa tgcagaaatg
    10801 agacaagttt tagaagatgc agttttacaa gcgtatcatg aagcattagt tcaatgggaa
    10861 gtagctgctg aataataata aatatataaa atggagagag cccgaacatt taggctcttt
    10921 ttaaatgtag aatttctatt aaaatatgta ctttttagta aaaaagtgct atattattag
    10981 tgataatgta tatgttgtat acaaattgtt agataaatgt attataatgt atattgatta
    11041 agagaataat acgaataaaa ttaaattaga ttgaatggag tgtatcaatg aactttaaag
    11101 ctataatcct tgctgctggc aaaggaacaa gaatgaagtc taaataccca aaagttatac
    11161 ataaagtatg tggaaaagaa atggttaacc atattataga tgtttcaaaa aaatcagggg
    11221 taaaagatac tgtcgtgatt ttaggacatg aagctgatgt agttaaagaa aagcttgcag
    11281 aagaaattat aatagccatg caaacagaac aacttgggac aggacatgct gtaaagatgg
    11341 ctaaagaaca tattaatgat gaagatacaa tagttgtact ttgtggagat actccactta
    11401 taaaagaaga gacactaaag agactatttg aatatcatat agaaaataaa tatcatgcta
    11461 cagttcttac aacaagagtt ggaaatccaa caggttatgg tagaataata agagataaga
    11521 agggtgacct tcttaaaata gtggaacaaa aagatgcaaa ttcagaagaa aagatgataa
    11581 gtgagataaa ctctggaatt tattgtttta atggaaagag tcttagagaa gcactagatt
    11641 tgctaaataa taacaattca caaggtgaat attatttgac tgatactgct aaaataatga
    11701 gagataaagg attaaaagtt ggtgcatttg caggttcaac tattgaagaa cttatgggcg
    11761 ttaactcaag agtagaatta tcaaaggcag aagaaattat gagacgaaga ataaatgaat
    11821 ctcatatggt aaatggagta actataatag acactaactc tacctatata gaatctgatg
    11881 ttatgatagg taatgatact ataatatatc caggtgttat gttacaagga aaaacaagga
    11941 taggttcaga ttgtataatt ggaatgaatt catctataac aaattcagaa ataggtgatg
    12001 gtacagaaat aaaaaattca actataattg atagtaaggt tggagaaaat tctactgttg
    12061 ggccatatgc atacttaaga ccaaagagtg acttaggaaa taatgtaaaa ataggcgatt
    12121 ttgtagaagt aaaaaatgct attatagaag atggctcaaa agcatctcac ctttcttata
    12181 taggagatgc acatgtcggt aaaaatgtaa atattggatg tggagtagta tttgtaaact
    12241 atgatggtaa aaataaattt aaatcaatag taaaagacaa tgcgtttata ggttcaaatt
    12301 ctaatttagt tgcaccagta gttgtagaag aaaaaggata tatagcaaca ggttcaacaa
    12361 taacacatga tgtcccagat ggtgctttgg caatagcaag agaaagacaa gttatcaaag
    12421 aaggatgggt agaaaaaaag aatcaaaaag acgaccaaag taagtaatta tgtagtttat
    12481 tattttggct aaaaaattta gctcgaaatt attataaatt atattcatta gtattttaaa
    12541 attttcagga gggttacata aatgaatact agcggaagcg agattaaaat tattgcaggt
    12601 aattcatcga aagagttagc acaaaaaata gctgactata taggtgtatc agtactagat
    12661 tgtgaagtag gtacatttag tgatggtgaa atatgtgtaa acatgaatga gacagtaaga
    12721 ggttgtgatg tgtttgtagt gcaatctact aacagcccag taaatgataa cttaatggag
    12781 ttattaatct taattgatgc gttaaagaga gcatcagcag gtagaataac agcagttatt
    12841 ccttactatg gatatgcaag acaagacaga aaggctaagg caagagatcc aatcactgct
    12901 aaattagttg caaatttaat aactgctgct ggagcagata gagtattaac aatggattta
    12961 catgcagctc aaatacaagg atattttgat ataccattag accacttact aggtggaaca
    13021 atattagcaa attattttaa tgaaaagaaa atagaagatt tagtagtagt atctcctgac
    13081 ttaggaagtg ttactagatc aagaaaattt gctaatactt taaatggaga agttccaata
    13141 gctataatag ataaaagaag accgaaagct aatgtatgtg aagttatgaa cttaataggt
    13201 gatgttaaag gtaaaaatgt tatactatta gacgatatga tagatacagc aggaacaata
    13261 gttaatgctg caaatgcact taaagagttt ggagcaaaag atgtttatgc ttgttgtact
    13321 catggtgtgt tatcaggtcc agctattgaa agaattgcta attcagaaat aagtgagtta
    13381 atagttcttg atactataca acttcctgaa gagaaaagaa tagataagat aaagataaaa
    13441 acagtagctc ctttatttgg agatgctata agaatgatat tctctaatga atcagtaagt
    13501 aaattattct aatttattgt atttatgaga gttgtttaaa aggatataaa ccttttgagc
    13561 aactcttttt tgattttata cttatggaat gttatttttt tcttgttaga aaggtatata
    13621 ttattgtaaa aagagattat agtttaaaat tatgtatatt taagtaaaat aaacctaata
    13681 aagggtatat tattcaaaat attatgaata taaaaaatat tacaaaaaag tattgaaaaa
    13741 attcagatta tgtagtatta tgtaaatata aataaagaaa gaggtgtttc caatgggaag
    13801 gttagaagat gaacccctaa taattaaatg attcagtcac aatctaaagt gaatataaaa
    13861 atctattgaa agtttattta taagaattga aaatagtatc tattctatct ttagtgtttg
    13921 tgagagggtt ctggattata taagattact gtaaagagta attttgcaca ataagcatat
    13981 ctgtaaatga agagtttcgt ttatataatg attgtggctg aatcataact ataatagata
    14041 gaatgtttat aatagatgat tttataaaat actatattgt aatagctttt agctttcaaa
    14101 taaatattag taaaggatgg aaatgacaat gataaaacta aatgataatt tataaatatc
    14161 tccttacgat atataataat tgtagggaga tgtttctttt tttgtatagc ataatttttc
    14221 tagaaattct gtttcaatat gtaaaaagtt ggtatatata aatagtataa acagtgttgc
    14281 taaaatatgg tagatattat ataatataag tggatatgta tatacattat gagttatata
    14341 atttgaattt aataaaatag aaacgttgat ttatgttctg taatgtgggc accttggagc
    14401 atattgagtt agtggtgcaa ccggctatga aattgtattt atttataaat actattattt
    14461 tcatagcttt ttttatttaa aaaatggtta tatgttagaa ataaatttgg ttagtacgtt
    14521 taaataattt tacttagatt tttaaccaga tatattataa gtaagtatta attaaaaaat
    14581 ttaaggggga atgaaaatga agttaaaaaa gaataaaaaa ggtttcactt tagtggaatt
    14641 attggtagta attgcaatta taggtatatt agcagtagtg gcagttccag ctttatttag
    14701 taatataaat aaggctaagg tagcaagtgt tgagtctgat tatagttcaa ttaagagtgc
    14761 agcattatct tattattcag atactaataa gatgccagct acaacatcaa atcctgtaga
    14821 tttagaaaat ttaaaaactt atatggaaag tcttcctgat aaagcagata taggtggaga
    14881 gtatcaatta cttttggttg ggaataagtt agttttacaa ataaatgatg ctacattaac
    14941 aggagcgcaa tcaacgaagt tattgagtga tttaggtaat gataagatat acaaaactat
    15001 aggtagcgat gataagctta cagatttatt aactaccaat gaaaaattag ataataaggt
    15061 tctatattta gttcttatag ataatgctga gatggattca acaaaataat taaaactata
    15121 tttttataaa gaatattgta aatttaatga aaaatataat ataaaagaca ggaagaaaat
    15181 cttcctgttt ttttatacta ttttctttaa tgttcaatta tgaatgatga tataattgag
    15241 atagtaaagg agtgaaacct gtggctaaaa aagttagaat aggagacaaa ctggtcgaaa
    15301 aaggatatat aacagaagaa cagcttaaat gggcactatc agaacaaaaa aacagtggaa
    15361 aaagactagg agaatttcta gtacaagaag ggcttataga tagtaactta ctaataagtg
    15421 tattaaaaga actcttggat atagaaagta tatttttaga agggacagaa atagacactt
    15481 tagctacaaa gatggttcca gaaaatatat gcaaaagata tactgtattt ccattcaaaa
    15541 tagatggaaa taaaatatgt ctagccatgt cagacccaca agacagagaa gcagttcaag
    15601 atgtaaggcg tatgtcaggt aaagatgtag agatatttat atcaagtaca gaagatataa
    15661 acaaagcaat aggtcatgct tatgcacatt cagaaataaa taaggcaatg actgagtaca
    15721 acaaaaatag aactggtgga gtaagagaaa cagtaatact tgaagaagat gtaaatgctg
    15781 ctccaatagt acgattagta aataacatac tagaaaatgc tgttagaatg gaagcaagtg
    15841 atatacatat agaacaaagt gaaaattaca tgcgtgttag gtttaggata gatggaatgt
    15901 taagagaata tatgaggatg aactcagcac catataaggc agtaatcagc cgtatcaaga
    15961 taatgtcaga tataaatata tctgaaaaga gaataccaca ggatggtaga atctatttaa
    16021 aagtagataa taaacccata gattttaggg tttctacaat gccaactaac agggatgaaa
    16081 agatagctat gagggttctt gataagagta attttatggt tagtaaagaa gtactaggaa
    16141 tagacgaaca tggctctaag atatatgatg aattgataaa cactccttat ggtcttatat
    16201 tggttgttgg ccctactgga agtggtaaaa ccactacatt gtattcaatg ttaaatcaac
    16261 taaatacaga aaatagaaat ctattaacaa tagaagaccc tatagagtat gaattacctg
    16321 gtgtaaatca atctcaaata aatgaaaagg cagggcttac tttcgccagc ggacttagag
    16381 cttttatgcg tcaagaccca gatataataa tggttggaga gataagagat actgagacag
    16441 cagaaatagc aattagagct tctcttacag gacatttagt tttaagtaca ttacatgcaa
    16501 atacagcagt tggagcaatt tcacgtcttt tagatatgga tgtagaaagt tttttaataa
    16561 cttcatctgt tttaggtgta atatctcaaa gacttacacg aaaaatatgt gagcattgta
    16621 aggtttcata tgaagcagat attggagaaa aaaaggctct tggtattgat gtaaatgaat
    16681 cagtaactat ttatagagga aaaggttgtg aaaggtgtaa taatactgga tataaaggac
    16741 gtctaggtat atttgaaatg cttgaaatta ctccagaaat aaaagagtta atagattcat
    16801 ctgcaaatca aagagaaatt ttaaagatgg ctagaaaaca aggtatggtt agcttaaaag
    16861 aggatatagt taaaaaggtt ttaaatggaa agacaacagt agaagaaatg attagaataa
    16921 ttttgatgac tgattaaagg atgatattat gaaaagtttt aaatacacag taataaaaga
    16981 ggacggaaaa aagcaaactg atgtgattga agccaacgat tttaatgaag ctagaaatct
    17041 tcttagaaag agaaatctta gagttattga aatcaaagaa agcaaaaata aaaatataag
    17101 attatctagt agaaagagaa aaaaagactt aggagctgac cagataagcc acttttgtag
    17161 acagtttgct attatagtat cttctggtat aaatagtatt tctggattag aaacattagc
    17221 tagaaggtca actaacatta cattaagaga agaaattaat cgtatagtgg cagaaataaa
    17281 agtaggttct actattgcag attcaatgct ttcatcaaaa tcaaagtttc caaagttact
    17341 tggtgctatg gtagctacag gtgaagctac aggtaaatta gatgaagtat taaaaagtat
    17401 ggcttcattt tatgcaagtg aacacagggt taaacaaaag cttagaaatg ctgcaacata
    17461 tccattaata gttttgattt cttcatttat aatgattttt atatttacaa catttatggt
    17521 gcctaagatg attaattcaa ttacaacagt aggagcaact cttccactta taacaagaat
    17581 tgttatgggg tttggtatgt ttatgaagaa gttttggata atagtacttt tagttattat
    17641 attatttatc tatcagttta aaaaatatct aaaaacgtca gttggaaggg ctcataaaga
    17701 tagagttata aacaagatac ctgttttagg aaaaggaata aattgtatgg tagctacacg
    17761 attttcaaga gcattgtatc tatttgtatc aacaggatac ccattagttc aagggcttga
    17821 ttatattata gatagtgtaa ataacaccat ggcagagaag attttagcat cagctaaaga
    17881 tggaataaca agaggagaag gcttagcaga aaatttagag agatatacgt attttgactc
    17941 tgtgttggta cagatgatag ctatagggga gcagacaggg gaattagaga atataagtag
    18001 acagatggca gaattttatg agtatgaatc cgaaatttat ttaaatagga tggcctcaat
    18061 gatagagcct gttttaataa ttgcagttgg tattatagtt gcgatactgg ttgtatcaat
    18121 atttatgcca atgcttagta tatatgatgc catgtaggag ggaaaggaca aatgaacaag
    18181 gtttatgtat catattatgg cgattctatt tccatagttg agggcagata taaacacgat
    18241 aaggataagt tttatgtaaa aaattttaat gtgttttcaa tagaggatat tgcacaagat
    18301 ttttctaagg aaaagtatgc tctattgaga tacgcattag aaaatgttaa gttaaaatca
    18361 agacatgtag ttttttgttt aaatacaaga gatgtaattt taaaacccca aaaacttcct
    18421 aatatggaca aaaaggattt agatggattt atgaagctag agatggacga aatgatggct
    18481 ttggagtcag atgaatatgt attttcgtat gaagtatcta gtgaaacacc tgataattca
    18541 gatggcgatg aatcaagctt aaatcttatc attggagcta tacaaaagga agaggtaaat
    18601 gcaattgtag agataatgca tgaatttgat ttagtattag atagaataga tacattatca
    18661 acagcttact taagaatgct taaaaattta gactacaatg atataatggt aaccaatata
    18721 agtgacaata gcacaattat aaatatatat aagaaagata ctttatttat tagtgataat
    18781 atacctataa agataactgc taaagatatg gacatgcaat tattaaatgt agtagaagaa
    18841 tcaaaaggtc ttatgaatta ctattcgtca agaaactttg gcaaggttac ggatactata
    18901 ttaatattag gaaaaagata ttccaataaa ttaatttatg aaacttttag ctctgcattt
    18961 tcaagctatg tttctcaagg ccttactgag ataatggata tatctgatat gataactgga
    19021 gatatagatg aagatgatat aaattcaatt gtagagcttt taggaagtat gatagaacct
    19081 aaagggaaaa gagattttga aaatataaac tttttaccaa tggatatact tagaaaacag
    19141 ataaaagcta aacaaatgaa aaacatactg aaaattgctc caatagttat tttgattctt
    19201 gctatacctt atttatgttt aattttttca aataatttag caaataagga acttgaaaat
    19261 gttaatggtg aaataagaaa tattgaatct gcatattatc agattggaaa tatagaaaaa
    19321 atgatacaga gtaaaagcga agaaataaag gtatatgata tgttaattgg gaaaaaggta
    19381 aagtgggaaa atttattgga tgatatagat aaaaatactc caagtaaagt tgaactaaca
    19441 agtttatcaa caacttatca aaatcttgga actaataata aagctgataa ttcaaaaagt
    19501 aataagacta caacagataa atcaagtaac aatgaaacaa aatcaagtac gaaaagtgat
    19561 agtaatggag ataaaacaga taatgattta aatgaagata agactacaaa tgtgtcagaa
    19621 gcattaactg gaaatagtga gactaatagt aataacacag ataaagatga taaaactcca
    19681 ttgtatgata agataccaaa tgttataaat atagaaggaa atgctaaaga ttcctcttat
    19741 gtaggtcaat ttttatataa attaaaggga ttatactatt tcaaggatgt taaattacat
    19801 agcataaaaa gtaatgcaga agattctaac tatacattta gtatgacatt atacctgaaa
    19861 gaaggtgtat taactaatga ataaagatat atttaaaaaa gatataaaag atatatttaa
    19921 aatggaagtt agtctaaaag ggattcttat aaaggttata attttattag ctattttatc
    19981 atatggttat tttggacttt accctcaata tcagaaatat attgctacaa aaaattctgt
    20041 aaaacaggct actgaaaaat taaatatata taaagagaaa actgcactta tgccaaaata
    20101 tgaaaaacaa cttgaagaag ttgaaagtga attaaaagaa aaaagcaaaa atcttttata
    20161 tgatatgcaa gatggtcttt ttttaatagg tttagataaa aaaatgaagg cttataatat
    20221 tgacttaacc agttatgacg ttggaaatgt taaagaatac gaacattttt atggtatatc
    20281 aacaacaata agtgttagag gtgattatag aaatgtcaaa aaattaatgg cttatttaga
    20341 agaacaaaaa aatgttactc aaatactaga ttatacaatg acttcatata tagaaccaga
    20401 aaaagaagag gaagaaacaa caaacaataa ggatttaatt aaaaatgtga acataaataa
    20461 agatacaaaa atatactggg tggaaggcga aaataagtgg catttatata aggattgtcc
    20521 attgataaaa gagggagaga ttctaaaaaa tgacaaactt ttagataggt ctactaatta
    20581 tctatgtaat acttgtgtac ttagagctgc aacagaaaaa gaagcaaaaa aagaagtcgt
    20641 agctccaaag gcagaaggtg tagtagatgc tacatttaaa tttataatgt acactaaaga
    20701 gaatactaca atgatacttg atacagataa tccatcttca tggagaccag gaaaatataa
    20761 tccatttaag tctagtgcga aataaagcta agtaagcttt aaagataggt gatgagtttg
    20821 tacaaaaata atgaaaaagg tcttacttta ttagaagtaa taatagctgt ttttatactg
    20881 acaatagttt taagtatttc ttataaagtg ttcaatggta taacatcagc agtaaaaaag
    20941 caacagatta ttacagatgc tcaagtaaat attaatctaa ttaataagta tctaaataga
    21001 gatttggaaa actgtaaaga actaactaaa actggttcag gtaataatta tgaatacaat
    21061 atagagatgc cagataatgt tgtaaaatat gaagtttcta tagaaactaa aaaaaataca
    21121 gaggtatatt ctgtaacgag aatccaaaac aatacaattg atacagaaaa tgaagttaga
    21181 gaagagataa tctataatca accactagtt caaaacaata aagaaatgaa ggaaacacca
    21241 tttaaaatag aaaagcaaac tggtaaatct atttatactg taagtatata ctataatgaa
    21301 tcagtgcaag aaagtcataa aaatagcaat ttaaataaca aaacttatac atttgatgtt
    21361 atgtcaagaa taggataata agaatttatt aaaaaataag taggttgata aaatatgagt
    21421 aaaaagaata gtgaaagagg attttcctta attgaagtgt tagtagctat ggctattatg
    21481 ggaattgtac tatttgcatt ttttaatatt atcaatacta ataacaaagc aaacactaaa
    21541 aatgacacag atataacttc cttaaactat gtccaaagtg aaatagaaaa tctaagagaa
    21601 aagataaaaa gtggagaatt tgattttgat agtttagata aactagaaga tggaactgtc
    21661 gtatatgaaa aattaataga taaatcaaaa aaagttgtat atgataaagt tcttagtgaa
    21721 ggtgatgtga gcttatatga tactccatat gaaaagatta caacaataaa agatgaagat
    21781 ggtaatttaa tcgacaaagg aaatattaca aataagataa agactattgt agaagataaa
    21841 agtggtcaga tatataaaat agctgtaact gggaaaagca tgaatgacta ttcaagtaaa
    21901 aaagaggtta aaattgtaac tgaaatattt aaagataaat aattttactt gggggtgaga
    21961 ttttgaggaa gtggaataaa tttaaaagtg aaagaggggc agctttagtt ttagttttgg
    22021 ttgtagtagc attgctttct attgttggat tgatattttc aaatcagata gctaatagaa
    22081 taaaatctac aaagactact aatgaaggta tacaagcaaa gtatttagca gagacatgtg
    22141 tggaaaatag tattaataaa gcatatgaaa aattgtatga tgagcttgag aaaatggata
    22201 atgaatttaa aagtgaaaat caagaaaaat ccatatctag gtcaaaatta agaaatatat
    22261 cagatgaaga ttttaataat caagatgagg aaaatattga agcagaaaga cttggatata
    22321 tgaataatat aaatttctat ttaaataaag cttcttcaga cttggaaaaa gctagtatgg
    22381 aattgaaaaa gctatatgat ttagatatgt tggactatag agacattgaa tatgtagatg
    22441 caaatattat ttcacataga gattctatct tagaaatttg caagaattat acttctggag
    22501 atattagtaa aataaatgaa tatattctgg aagaagatat agactcaact acattgattg
    22561 aggcaaagtt agtgaataat gatattcttt taaaaatgtt tttagaagaa aataaaattg
    22621 aaaatgaaca tttaaattca gccttttcac atacttataa ggcattagat aacatttctt
    22681 tggcgatgca aaatatgatt gagtatagac atacgtttca tatagatgag ccaaaagtag
    22741 aagtatctaa tgggattcca gattctcaac aatattatga actaatacaa aatcctataa
    22801 taaatagtat ggagtatatc tggaatagta aatgggatac attagaaaac ctattggaaa
    22861 ttttaccaga tcaaactcaa ggatttaatt ctttaagagt tcatttgaga aataatgtaa
    22921 gaaaatttga gaagttaagc gataatatat ctagtggtaa aaaaaataca gcaaaaaatt
    22981 tcttaaagta taaagagtta ttgtatgaaa tatcagacca atgtaatcaa ctaaaaagta
    23041 tgtcttatga gaaaatacct gttaaatatg ataacatggc tttgataaca acttttgact
    23101 atatacaaaa tgagctttta gcagagataa aatgcagatt aaaagaatta aaaccacaag
    23161 aaattgataa aacagaaggt attactataa aaataccttt ttataaggct gactacgaca
    23221 tgacaaaaga aggatggcct aaattaaaag aaaatggttc aggggcggag ttaagcttaa
    23281 tggtaactgg tgataaagat ggaataaaag aagtagaagt tacagatggt aaaaaaaata
    23341 ttattggttt aggtgttgaa gaaaattcaa attcaaagta taaggttgac gccattataa
    23401 actttaatct aaatatagat acaaatgtag ttggaaatta tgatataaaa gataaaattt
    23461 tgattaatca tgatataagt tcatacaaaa aagtaaacta aatataagaa gcaacttagt
    23521 ataaataaat aataaaataa ttttattatt tactactaaa aaaataagta aactaataga
    23581 agatatggag agtaaatatt atgtatattt atgatttatt agagcaaggt ataagactaa
    23641 acgcatcaga tatacacata accgttggaa caaatcctgt agctagagta aaaggtggat
    23701 ttgtaaaact aagtgaacaa atattgacat cagaagtaac aatgcaaatg gcaaaagaca
    23761 tagcggggga atctatgttt aaagtaatag aagaacatgg agaagctgat ttttcagctt
    23821 ctctaaaaac aggagaaaga tttagggtaa atgcatatag gcaaaaagga aattacgcaa
    23881 tagcaataag gactataaca gcagaaattc caacatttga aaaattaggc cttccagaga
    23941 gtataaaatc ttttactgaa aagcataaag gattagtttt agttacaggt ccaacaggaa
    24001 gtggtaaaag tacaacactt gcaagtatga taaatataat aaatgaaaaa caacaaaaac
    24061 atataattac acttgaagac ccgatagaat atgtacatca tcataaacaa agtttagtaa
    24121 atcaaagaga agtaggaact gatacagaaa gttttcattc agcccttaga gctattttgc
    24181 gtcaggaccc agatgtaata ctaataggag agatgagaga ccctgaaact gtttcaatag
    24241 cattaacagc agcagaaaca ggtcatttag tattttcaac actacataca gtgggggcag
    24301 ctaagacaat tgatagaata gtagacatgt ttcaaccatc ccaacagcaa caaataaaaa
    24361 cacagttatc tacagtttgt gaaggtgtgg tatcacagca gttacttcct acagcagatg
    24421 ggaaaggtag aatagcagca attgaattga tgtttgctac tccagcaata aaaaacctga
    24481 taagggaagg caaaacatat caaattccaa acatgataca aactggagtt aaagtaggta
    24541 tgaaaactat ggaccaagat ttaatggaac tttataaaaa tggaaagatt acaaaagata
    24601 tggcacttag taggtgtact gaccaagagt ttatgacaag aatgataggt ggagtaaact
    24661 acaatggata ttataatcgt taatctatat gtatttatag tgggtatagt gtttggaagt
    24721 ttctttaacg tctgtatttt taggattcca gaaggtatat ctatttctag accaccttct
    24781 cattgtatga gttgtaatac tagactaaag ccaatagact taataccagt gctaagctat
    24841 ttaatgagtg gcagaaagtg tagatattgt tatgagaaaa tttctagtag atatgctata
    24901 gtggaattaa ttacaggact tttattttta acagtattta tgttatatgg aatatctaca
    24961 tcaactattt attatttggt tcttgtgtct ttactgatta taattacatt tatagatatt
    25021 gaccacttta taataccaga taaaatattg gtttttggag ctatattttc tatgattttt
    25081 aaccttttat ttaaagaaat tccagttaag gattcaattc taggtgtttt tatttgtgga
    25141 ggaagtgtct ggattatagt tcttttgatt gaatttgtaa taaaaaaaga gtgtatgggt
    25201 ggaggagata tcaaactatt tgcaatgcta ggactttaca tgggtgttaa aaatggtctt
    25261 ttgacagcct tactaagcgt ttatgtagga gcagtctatg gaatctgtgt aataattata
    25321 agtagaataa aaggaaagga atataattct gttataccat atggaccttt tatatctatt
    25381 ggagcattaa taacaatctt atctggaaag caattacttg aactttattt ttctatggtt
    25441 atatagatga tagtcaagtt aaataaagtt
//
LOCUS       DAELJO010000034        25468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELJO010000034 DAELJO010000000
VERSION     DAELJO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.034"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            114..965
                     /locus_tag="KPB88_001266"
     CDS             114..965
                     /locus_tag="KPB88_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0053563.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRIIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            969..1439
                     /locus_tag="KPB88_001267"
     CDS             969..1439
                     /locus_tag="KPB88_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053564.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1452..2762
                     /locus_tag="KPB88_001268"
     CDS             1452..2762
                     /locus_tag="KPB88_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053565.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            2974..3720
                     /locus_tag="KPB88_001269"
     CDS             2974..3720
                     /locus_tag="KPB88_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0053566.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4082..5623
                     /locus_tag="KPB88_001270"
     CDS             4082..5623
                     /locus_tag="KPB88_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0053567.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5658..6764
                     /locus_tag="KPB88_001271"
     CDS             5658..6764
                     /locus_tag="KPB88_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0053568.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6974..8260)
                     /locus_tag="KPB88_001272"
     CDS             complement(6974..8260)
                     /locus_tag="KPB88_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0053569.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(8383..11481)
                     /locus_tag="KPB88_001273"
     CDS             complement(8383..11481)
                     /locus_tag="KPB88_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0053570.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYNIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            12185..12337
                     /locus_tag="KPB88_001274"
     CDS             12185..12337
                     /locus_tag="KPB88_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053571.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(12629..13333)
                     /locus_tag="KPB88_001275"
     CDS             complement(12629..13333)
                     /locus_tag="KPB88_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053572.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(13324..14931)
                     /locus_tag="KPB88_001276"
     CDS             complement(13324..14931)
                     /locus_tag="KPB88_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG0053573.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15185..16363)
                     /locus_tag="KPB88_001277"
     CDS             complement(15185..16363)
                     /locus_tag="KPB88_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053574.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(16589..17083)
                     /locus_tag="KPB88_001278"
     CDS             complement(16589..17083)
                     /locus_tag="KPB88_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0053575.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17084..17845)
                     /locus_tag="KPB88_001279"
     CDS             complement(17084..17845)
                     /locus_tag="KPB88_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0053576.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            18567..21401
                     /locus_tag="KPB88_001280"
     CDS             18567..21401
                     /locus_tag="KPB88_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053577.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNTTIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FNPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            21444..22688
                     /locus_tag="KPB88_001281"
     CDS             21444..22688
                     /locus_tag="KPB88_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0053578.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERSHRLIGVVLG
                     DGNKKNRISSSASLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(23191..25191)
                     /locus_tag="KPB88_001282"
                     /pseudo
     CDS             complement(23191..25191)
                     /locus_tag="KPB88_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         8711 a         4020 c         3379 g         9358 t
ORIGIN      
        1 gaaacttatt tgaaatagtt ttttcatttt atttaggaaa acgtattata cttaagaaaa
       61 cgtattttct catttcagat aagtaaaata tttattcttt tgaggaggaa atcatggaaa
      121 ttaataaaaa acattttatt caagtatttt taacttgctt aatcttggta gtaatatgtg
      181 aatttatagg gcaaagaatt atagctattg gaaaagcctc tatagtactg ttccctatgc
      241 tttatgcagt tataattgga cttataataa caccagattt attaggtaaa aaaattaaag
      301 cacttaaaaa agtggttgga gaaaaagaaa taaatattgc tggtgaagtt gttggaattg
      361 cactagtaat gcttggtata aaatatggta ctacagttgg accaaacatt gacaaaataa
      421 ttcaagctgg accagcgttt atcgctcaag aatttggtca tatacttgca ccaatcgttg
      481 cattacctct tgcattaatg tttggaatga aaagagaagc tgttggagct actgcaagta
      541 taagtcgtga accttcttta ggagttatat cagaaaaata tgggataaac tcaccagaag
      601 gtagtggtgt acttggtaca tatttaatgg gaacagttct tggtactata tactttagtg
      661 tactaggttc aatatctatt tatagtggtc ttcacccata tgctttggga atggcctgtg
      721 gtgttggtag tggaagtatg atgacagcag ctgctggttc attatctacc atggtaccac
      781 ctgaaatggc agatacaata cttgcctatg ctgcaacaag caatatgatg tctagtataa
      841 caggaattat attcttagtt tttgtatcct taccatttac taattttatg tataagattt
      901 tagaacctaa attttcgaga aataaaaaaa ctaaagaaat taaatctaca gaaggagagt
      961 gctaataaat gtttgctaat ttaaatttaa agaaaagttc atatcaatta ttggttatgt
     1021 ctgttattgg agttataatt cttataggac aaagaattag cgttggtaca tctatagtta
     1081 cagctcttcc tggtatggtg atgctaatat tagctgctat ggcagctatg attataaaag
     1141 atttatttcc taaatcaatc tttccagctt ttggatttgc aactataatc ggccttttac
     1201 ttagtatgcc atatagtcca acatctgaag tattcttaac taacactaat aatattaact
     1261 ttatggctat aactactcca ctactagctt ttgcaggaat ttctgttgga aacaaaatag
     1321 aagctttaaa agaaatgtca tggaaaatag ttataatttc acttattgta tttacaacta
     1381 tattctttgc atgtgcgagt atagctcata tagttttaag tattcaaggt aaaatatagg
     1441 aggaatttat tatgaataaa gaacaattaa aatctctagt aataaaaact atagatgaaa
     1501 atagagaaaa aatattaaaa ataggtcaag gaatatacaa aaatccagaa tatggatata
     1561 aagaatttaa aactacagaa gctgtttcaa acttttttga gcaagaatta aatttatctg
     1621 tagaaaaaaa tatagcagtt acaggatgca aagcagatgc aaattctagc aagaaaggtc
     1681 ctcatatatc tatacttgga gaattggatg gtatatcatg taaagaacat aaagatgcta
     1741 atgaaatagg tgcttctcat acttgtggac ataatattca aatagctgga atgttaggtg
     1801 ctgctgttgg tttagtaaaa agtggcgttt tagaacattt agatggtaaa gtatctttta
     1861 tggctactcc tgctgaagag tttattgagt tggaatacag agagcaatta aaaaaagaag
     1921 gaaaaataac ttattttggt ggaaaacaag aattaataaa aagaggttat tttgatgata
     1981 tagacatatc tatgatgttt catgcttctg atttaggaga agataaggct ctagttggtc
     2041 ctgagagtaa tggatttgtt ggaaaaaagg ttaaatttat aggtaaagaa tctcatgctg
     2101 gttctgctcc acatgatgga ataaatgctt taaatgcagc aatgcttgct ataaacaacg
     2161 tgaatgcact tagagaaact tttaaagagt ctgaaagagt tagattccac cctatcatca
     2221 caaaaggtgg agatatagta aatgttgttc ctgctgatgt tcatatggaa tcatatgtta
     2281 gagctagaac tataaatggt atgattgatg ctaatgaaag aataaataag gctttgatgg
     2341 ctggtggaat ggcagttgga gctgatgttg agataactga aattcctgga tatcttccaa
     2401 tattacgata caaaagtctt gataatttat ttaaaaacaa tcttgaagat ttaggtctaa
     2461 aaggaaaagt tattgatggt ggagatttta caggctcttt tgattttgga gatgtgtctc
     2521 atataatgcc tactcttcac cctatgattg gtggtgtaaa aggagctctt catacaagag
     2581 attttgaaat agttgatgaa gatttagctt acataattcc tgctaaagca atggcactta
     2641 cagttgtaga ccttcttttt gataatgcta aggatgctaa agatatatta gataccttta
     2701 ctccagttat gactaaagaa gaatatctaa cattcttaga aaaacacgat aaaactttat
     2761 aagtctattt ttataatagg gtggtatcta ctgtaaaagt tataccactc tttgtttttg
     2821 taacagtata cttcttttct tttagttttt agattaaact aaaatatatt atcctcctta
     2881 aaacttttag agctacgttg aaatatcagt agaactatgc tatattcttt ctaattaata
     2941 taaatttcta ttctaattca aggtggtgaa aaaatgttta atgcaggaaa tcgaattata
     3001 aatacatggt tgtaccctat tcaagatggc tttgtcttga ttgatactgg atatgaaaat
     3061 ggttttaaac atttcaaaaa acgaatttct aaattcaaca taaaaatcaa agatattcga
     3121 tacatatttc taactcatgc tcatgatgac catgttggtt ttctaaatca gattctagac
     3181 gaatctccta atacaaagat tgttatgcat gataaagctt tagaaattct atataaaggt
     3241 caaaactctt ttattggtgg ttgtaccagc cgtactgcct ttctcttttg ccaaattatg
     3301 aaactatttg gaaaaggtga gcatagtttt tctccactta aacaagaatt tgaaaaaagg
     3361 tgtattcttg tgtctgacaa gaatcgtaaa gaaattgaaa aagaactaaa tggaaaaatt
     3421 atagatacac caggacatac tgatgattca atttcactac taattgatga aggtgtttta
     3481 ttttgtggag attctgcaat gaatggattt ccaagtactc acaaaattac catatttgca
     3541 gaaagcaaac tagaatttat tcaatcttgg aaaacaatca tcaatacaaa acctaaaatg
     3601 atttatcctg gacatggaaa accatttgaa tacagtaaat tagaacaaaa tctaatctat
     3661 gtaaataaaa taaatttata tcctctacat aattcaaaag atattcacag atttttgtag
     3721 aaaatacaaa gtaaatttat ttgattaatt aaaaaatcta acactttact acgctactta
     3781 ttttaataac ttaatcttcc actagctttt cataaaattt tattttaaaa ttaattttaa
     3841 agtgcatatg ttatttaaat acattagata aaattcgtca ttaatctact taaagcatat
     3901 ttaactaaca ccactcaacc agaaaattca gaaaactgca ttgacacagg aaaaagttta
     3961 ttttatactt tttttaggac atattgtgga cacgtcttaa atcgacaaac agaatttaaa
     4021 ttgtaagatt aaccacaata cttatttttt aattatattt ttaaacatta gggaggatat
     4081 tatgaatgat aacgtagtta agaagaaaaa cttttttgac aggacattaa acagaataga
     4141 aactgttggt aataaactac cagacccagt tacaatattt ttagggttat gtgtactttt
     4201 acttatacta tctagtctag ttggttctat gggtatatca gtagttcatc ctggtactgg
     4261 agaaactata accgctgtaa atttattgac tgtagaacag cttcaaattc ttttaggaaa
     4321 catagtcagc aatttccaag ggtttgctcc tttgggtcta gttctagtaa ctatgatagg
     4381 tgctggtgta tgtgacaaaa caggacttat gacagcaaca atcaaagcat cagtaagcaa
     4441 gattcctgaa acaagagtaa ctcttgttgt aatgactata ggtatgcttg ccaatatagc
     4501 ttcagatgca ggtacaatat tatttccacc attagctgca cttgtatatc ttggtgttgg
     4561 tagacatcca cttataggtt tattttctgg atatgcagca gtctgtctag gatttgccgc
     4621 aaacattatg ataagtgtca atgatatact tgctgcaagt tttacagttc cagcagctca
     4681 aatgattgat tctggatttc aagccaatgc aacaatgaac ttatttttta tgatagcatc
     4741 aacttttgtt ctaatacttt tggcaacatg ggtaacagaa aaaattgtag ctcctagatt
     4801 tggtaaatat gaaggaaatg cagaactaga tgttgatgga agtcttagtg atgtagaaaa
     4861 gaaagggctt aaaaaagcta gtataagtat attaatatat gtggctatta tagtagctct
     4921 tagtgtaatc ggtaaaaaac cattcttagc tgacccagaa acaggagatt tactatcaaa
     4981 caatgctcct cttatgaaag gtatggtacc tataatagca ctagcatttt tcatacctgg
     5041 tgttgtatac ggaaaaacta taggtaaaat aaagaaggat aaggatgtag tctctataat
     5101 ggctagtgca atgagtgata tgggtggata tataatacta gcatttgctg cttcacaatt
     5161 tttgcaatta ttcacaaata gtaatttagg tctgatacta gctgtaaagg gcggagaatt
     5221 tttaaaatca gcaggaataa atggaccttt attgttgata ggatttatta tattatcatg
     5281 ttttataaat ttatttatag gtagtgcatc tgcaaagtgg gctattcttg cacctatatt
     5341 tgtaccaatg tttatgatgg ttggatacaa tccagcactt actcagatgg cttatcgtat
     5401 aggtgattgt ataacaaatc cacttagccc attatttccg tacttcccta ttatactagc
     5461 attcacaaga aaatacgata aagatgctgg tatgggtact gtaattgcta atatgatacc
     5521 atactctata tcattcttaa tagtgtggac tatactatta atattgttta tggtatttaa
     5581 tattccacta ggacctggaa tacttccttc atatagcatg taatttattt aaatctttct
     5641 aaaaggagaa ataatttatg ttagataaaa atagacttat aaataatttt atggatatgg
     5701 ttagaattga tagcccatct aatcaagaat tagaaatgtc taagtggttg gtaaactact
     5761 taaaagaaag aaatatagac gccattatag atgatgcagg taaaaaatat ggtggtaata
     5821 caggtaatgt cattgcctat ataaaaggtg aagaaggaag tagaccactt tgtttatgtg
     5881 cacacatgga ccaagttcaa ccttgtttag gtgtaaaacc aatactagat ggtaatgtag
     5941 ttagaagtga tggaactact acacttggtg cagatgataa agctggaata gctgctatac
     6001 ttgaagcttt agaacatgtt attacagaaa aaataccaca tagagatatt tatctttgtt
     6061 ttacaatctg tgaggaagct ggtatgcatg gcgtcaaaaa ctttaatcct gacaacttgc
     6121 cttgtaaaga tatggtgatt ttagattctg gtggagctat tggttcgata gcatacaaag
     6181 ctcctgctca acaatctata aaaatctctt ttcatggaaa gaaagcacat gctggtatag
     6241 aacctgaaaa agggttaaat gctatattag tagcctctca tgcaataagc aacatgcaca
     6301 taggcagaat agattcattg actacttcaa atataggtaa aatagaaggt ggtggagcta
     6361 caaacatagt aactgataag gttacattga ctgctgaaat taggtctcat atacctgaaa
     6421 ctttagagta tgagcttaat catatggaaa aatgttgtaa ggatgctgca tctaaattta
     6481 atactactta tacttttgaa cataatatgt cttaccctag ttttgaactt agtagagata
     6541 gtcacgtttt caagttaagt gaagaagcaa taagacaagt tggtgttgtt ccaaatccaa
     6601 tggtaattgg tggtggaagt gacgcaaata tattagctaa tcttggatat aattgtgcta
     6661 tattaagtct aggtatgtat gatgtacaca cagttaatga atatgtaaat atagacgaat
     6721 tatatgatac tgccaaaata gtttatcata tgattaaatt ataaatatca taatagaact
     6781 ctttaaatta taaaaataaa aacctatgta tcttcaactt tataagttat atattagagt
     6841 tgttttacat aggttttagt atttttataa taatttgttc taattttttt cataataagc
     6901 tttaatactt tatatagtaa tttgttgtag aattttatac aatataatga tttatacagt
     6961 tttataaaat atcttaaaac agtatttcag taatattttt tggtcttcct ccaccaatac
     7021 tagtctcttt tgcacatatt cgacctaaac ctgcatttac tattttattt aatatacgtc
     7081 ttgcacttct ttcacttaca tttagatact ctgctaactc ttttgaatca taaatcttac
     7141 tttttctatt ctcatttata gcaataattt ttgcaactga ctcacaactc attccagtct
     7201 ttttagatat ttcgattacc ttttcatcag atagcatcaa agaataattt aattcatttt
     7261 cggaagctaa aggtcctctt atgttttcat cttcatctac caaatatata ccaatttctt
     7321 ttgaatctat ggagtgtctt aatgctttat gtgcattatt ttcagcctga tacgcagtta
     7381 ctccagtacc aattcctata ctaagagaaa agcccatgtc ttttatctct ctctttagat
     7441 tgaatagctt atcataatta tactcattat cagctacccc tttatgaaca aatacaacat
     7501 aatcatttct tccaagtaaa aataaggaac cttgaatgct tcttacatag tttactacaa
     7561 gcttatccat atctgatttt tttatcatgt cagaataata gttatctatc ttctctttat
     7621 aatctattaa actcaatatt tcaacagcaa tttgagaaaa tcttgcctta cttagagcat
     7681 attcactctt taccttgtca taactttctt tgattaattg tatagtagct tgtaaacgaa
     7741 atacaggata tcctctttct tttaattcat tatatactgc accaaaacca gtcaacatta
     7801 tatccgtctt attttcttca aataatttta tatgccattc tacaagttca gattctccta
     7861 cttccaaaga aaatggatta caaaatacac tcgttgcttt aatgtcaaat tctttaagtg
     7921 catcttccac aacatatcta tcaactacat ctatactgaa tttatctaat tttatatttg
     7981 cactttttat tgcccaaaat gccttcatta tactcgtccc acttctcggt acaaatgaat
     8041 gtggcttact tatctcatac ttagatttta taaattcata aactgcacat cctgtaaata
     8101 taatagcatc acattcgtct tcacatttag aaataacttc tattgtatct actacttttt
     8161 ctcttacata tattttagtc tttaaatcac tgtctatctg ttttaaatca tcctttatct
     8221 taaacccaga gtctgaagga cctataatac ccactatcat aaacttcgct cctaatccat
     8281 aaatattata ctttttaaaa attatatcct cactattttc tacttctatt taattatttt
     8341 gaactactcc atcctaccta catttagaat ggaataatta gcttattttt gataaagtaa
     8401 cttctcaaaa tcagcacttg gaataggctt acaataataa taaccttgaa ttaccacaca
     8461 atctaaactc actaaaaatt cagcttgttc ttttgtctct acaccttctg ccactacatc
     8521 catatctaaa tcttttgcca tggctatagt gtgctttaaa actatttttt ctctgtctgt
     8581 gattccttcc tcattaaaga aaccacaatc tatctttaat gtatcaatat taatattttt
     8641 taacatattt aatgaagaat atgctgaacc aaagtcatcc atatttaaaa caataccatc
     8701 cattctaagg tcggctatta tcttagtaaa taattttata tttttaataa ataaactttc
     8761 tgtaagctct aattctaaaa aagattttgg tatactatat ttatttatta aacttaaaac
     8821 aatttcttta aaatgtggat tatgcaaatg taatcttgat acatttacag agataggaac
     8881 tggctcttca cctaaatcta tccattttct aatcaaagaa catacttgtt cccatacata
     8941 catatctaat ctgataataa acccattctt ctcaaatagt gaaataaatt taattggagg
     9001 tattatacct ttttctggat gaacccatct aaccaatgct tctgcaccaa caattttatt
     9061 tgcattaaga ttatacttag gttgtaaata tacatggaat tgcccatttt caagtgcgta
     9121 ttccatttcc ctttccatct gtttttcttc aagtgcatca tttcttatag tatcatcata
     9181 aaatatatag ttgttgatac tttgtttctt tacttttttg tgagcatatc ctgccatttc
     9241 acacgcattt gtaattggaa tatgattatc caaaattcta taaactccaa aacatggtac
     9301 taacatatat tcaaattttt ttctatttaa gctattttta atattataaa ttaccttatg
     9361 aatatctcta tcagagtaac tctcataaaa aatgcaaaat acatctgcat acatacgaca
     9421 acatatacca ttagaaccaa tgtttttttg aatcaactct gcaacatata ttaaagcttt
     9481 atctcctcct ctaactccat aaatgtcatt aattaccttg aacttatcta tatccatcat
     9541 tataattgca taacgcttgt caggatatct tttaagcatt aaacttattt gttcacaaaa
     9601 actttcataa ttgtatagtc ctgttaatgt atctttttgg tttttatgta gttgcataag
     9661 ataatctata ttttttatag caactaaaat atgtgtttgt ttctcaccta acttattttt
     9721 actactaatc tttaacatat aaattaatgc ccaatagtac tttttatcta ttcctaaacg
     9781 tctatactca aaatttagtt ctaaatcgtc ttgcttgcta ttattaagac tatcaagaga
     9841 ttttagaagg tggttatcat taaataatat tatatcatca ggatgaatta aatgtttttt
     9901 tatgtatgaa aaagtttttt caagattttc ctttatagga aaactaaaaa tattgctctc
     9961 tgattgataa attggagtaa ttaaattcgt atcaatgcaa atagataaaa ttatatcata
    10021 tgtattataa gctaattttt cataagcctt agaataccct tttatcatta aatcatcaaa
    10081 ataaacctta tcaactaact ctttatgttt aaataaaata ttattcgtcg aatcagctaa
    10141 gtcactaatt actttcattt cattatttct aagtttgttc ttattataat acctcttctt
    10201 atcttggtac attctctcat cagcaacttt aattagctta tgtatatcaa tattatcgtc
    10261 actccaaata ctgcccactg ctacactata tatcatctca tccttaaatt cttcacttaa
    10321 ccaactcact aattcttcaa atagttgttt tgatatatct tcacataata caacaaactc
    10381 atctcctcca atacggtaaa cattataaga atgaaagtat ttttttatta ttacacatgc
    10441 tgacacaata gcagaatcgc cattagcatg tccaaagtta tcattaatat cttttaaccc
    10501 atttatatct acaaatacaa ctcctatgga tttaactttt cctttttgaa aattatctat
    10561 atacttaata tatttatttc tattatatgc accagtcaag aagtcatagt agctcatata
    10621 atttaattta ttttctactt ttcttttttt catctcagtt aataaaaaat aagataggga
    10681 tgtcaaaaga gaggtatctc caacagcttt atgtggatta tcaactccaa taaaccctac
    10741 tactttatca tcttgtttta gtgaagatgt cattaaactt tcaatttttt gaggttttaa
    10801 aatttcatat tctattggct gttcttcttt tatattttct atatcatcaa ttattacaaa
    10861 acctttttgt tcgaataata aaaaccaatt ctttaattca ttaatatcaa gatttgttag
    10921 tgcctgtttt tgttcatcaa caccttcatt gcaccattca tatgtattag ttacttttgt
    10981 tctatcttct gatacttcaa aaacatatgc tctatcagct ttatgaaatt taccaatatt
    11041 ggttaataca agatttacag catcagaaaa attagattct gttataagat gtttaataca
    11101 tccaactaat aactccttta tctctaattt ttctgctatt gacttactta tatcttcctt
    11161 ttctgtaata tcaagagcta tttctaatcg tgcatgtttg ccctcccatt taattaactt
    11221 gtcctttaat ataaaatgtc tatttgttcg agtattcgta ttttcccata tgtaatattc
    11281 atcttctttt aaaatagagt ttgtacaaaa ttcgcaggga gaatctttcc cctgtaatgc
    11341 tttataacat tttaaatggg atatatcatt aagtcccatt gcctttttcc caatttcatt
    11401 aatatataaa agttcatatg tatctaaaga agaaacatat acaatttcat ccaactcatc
    11461 tattaaattt attacatcca tttatatata tctcctttta ttcattttcc agattgaatt
    11521 tttaaagctt ttaaaatttt gctatatatt tggcaatgat attatacttc tttatgtttt
    11581 aaaatagtac cttaatgagg ttctattttt tagtaaatag aattattatt gtgtcaatcg
    11641 tacaatagag ttttgtaata cttaatttaa tatatttata attaactatc ttattatgta
    11701 tttacaatta attctcatat aaatgacaaa tctccacctc aattaagtat tccaatcata
    11761 tatgaggata atacttcatt catttaaaaa tataaaacta ttatatatta aaatttcttc
    11821 aataataaat tatttagatt gttactttga tatattatat aatatcacaa aggctgatat
    11881 aaaaagacaa aaagggcaat ttttttatat caaaaaaaat gttaaattga tattatccat
    11941 atagatgcta tatttaacaa ttctacttac ttcccttaca cttattagca tatagttatt
    12001 aagagtttgt tgcaaaacaa cacttagcac actttaaaaa gtgtggtcta ataagtaatc
    12061 aacgaagaag acataataaa gcataaaata tgatatactt ttccacgtca tcaaaattga
    12121 ggattttatt attaatatat gataaaatat gcttaataaa ttaaatttta atgggagaat
    12181 agttatgaaa atatttagtt tcttgttgtt tgtagtggga atatgttttt gtattgctgg
    12241 atttatgggg cattcctcag ctttttctgg tgcaacattg agtttttttg caactacaat
    12301 gatagctgta agacaagtca aaactaagaa aaaatgaaat aggcaaatcc taactttaca
    12361 agttaatatt acataagtaa ttagtctaaa attggatact ttttctatta cagaaataca
    12421 tcatccactt tgtattttct taaaaaaata aaatagctat gtaaattcaa gattagatag
    12481 attaactctc tatccatttc attgtaaaat atacatagct atttgtattc gatttaaaat
    12541 acttagattt tcttacataa caaataattt ctatctgatt attactcata ttcatttagt
    12601 aaccactcag cacctaatca tttagtatct attcatccgc catacggtaa cctaccccaa
    12661 cctcagtcaa aatatattct ggctgagcag gattcttttc aattttcctt cttatacttg
    12721 ccataaaaac tctaagcgac tgattttcac tttcatagta atttccccaa actttatcta
    12781 ttataaactt atgggttaag actcttcctg aatacttaga catcaactca ataatcttat
    12841 attcgattgg agttaaatgt atttcttcac cattaattga cacaatatga ttttcataat
    12901 ctatctttag gtttttaact tcaaaagact ttggagcatc attatttata ttatcctgtt
    12961 gagctttatt tctaagagcc acacgtactc tagccaaaag ctctgttaca ctaaaaggtt
    13021 tagttaaata atcgtctgct cctccatcta aagcttccac cttatccctc tcatgctctc
    13081 tcgctgaaac aacaattatt ttattagtct ttgcacatgc cctaactcgt cctattacct
    13141 caatcccatc aatatctggt agtcctaaat ccaaaattat caaatctggg tcaagagaat
    13201 gtataagtga tatagctgtt cctccattat ctgtctctac acacctataa ttttgagttt
    13261 caagagatac ttttataaat ttacaaattg gcttatcatc ttctacaatt agaactaatg
    13321 gctttatttc cacttgtctt tcccctcctc atcatacata ggtatgctaa atctaaatgt
    13381 agctcctcca gaaggattat taaaggcgac aatttctcca ccatgagctt ctactataga
    13441 tttacatatc tccaatccaa gaccaatacc tcttctttca agatttctag acttattctt
    13501 agtataaaac ctattaaaaa tattcccgat atcttcattt ggtattcctt ttccattatc
    13561 tgcaacttca aatagtactt gatttgactc tttttttaca gttagttcta tcttagaatt
    13621 tttaggtgta tgccttatag cattatcaat tagatttact aacacttgtt ctattaataa
    13681 tacatccaca tgaacgatta ttatctcatc tggaatattt gtttttatgt ctctactatt
    13741 ggcaaacttt ttgactctta atatagattc agctattatt tcatcaacaa cttctggtcg
    13801 tttttcaata tccaattttc cttcatctat tctggtcata ctaagtatat tctcaacaga
    13861 atgagttagc caactcgtat cttcatatat atttttcaat aattctactc tagtttcttt
    13921 atctatcaca tcatcatttt caagtattgt tgaagatgaa cctaatatac ttgtaagtgg
    13981 agttcttaaa tcatgagaaa ttgacctaag caagtttcct cttaatctct ctctctctgc
    14041 ctcaagattt gcttttttat ttttctcaaa taaattttct ctatcaattg ccaacgcaac
    14101 ctgtgttgaa actgcttcta atagtatctt ttcattttct gtaataatat ctatcttaaa
    14161 acacgcaaca cctataacac caagaacatt tgattcttga ccttttatag ggaaataata
    14221 tgcactgctg tttttacaat attttgtccc aattccaact gggttacctg tcctaaaagc
    14281 ttcttttata gcttcctttt ctaatactga atggaatata ttctcacttc catcattgtt
    14341 acaaatataa atacttggct tacctagatt attattctca tctgcaatag tcactattac
    14401 agttctattt aatatattta ctaaattaga cccacaaaat tcaataattt gatttttatt
    14461 ttttgcttta agcaacgttt tgttattctc atagagcaat cttatcattt tttctctctt
    14521 atgagaaagt ctcacttctc tttttatcct agaagttaaa gtacttgtta taattgctgc
    14581 cattagcata atacaaaagg tcataggata atcttttcta taagctaaaa aagtatataa
    14641 tggatgcgta aagaaaaaat taaatgatag cactcctatt actgaagaaa gtattccaaa
    14701 aaaatatcca taggttatac ttgtagcaaa tagtacacct aatatgtata ctagtataat
    14761 attagattca ttaaaaccta ccttttcaaa aactactgat attccagtag caattgtcat
    14821 aaccaatata gtttttatga agtccttaat aattatcttt ttactgagat agccatcttc
    14881 atcataatca tactctttta agtcttcatg ttgtagtcgt tcctttttca tttactacaa
    14941 ttcctcctta ttgttactgt ttaaattggc tctattttat ataatacaat tttatcataa
    15001 taaattcaat caatacattt aattaatgta cttaaattac taaaaggtgc tgtcttaaaa
    15061 tgaaaataaa tctctatttc aaaacagcac ctatatattt aaattactaa tttagttttt
    15121 acatacttaa atcaatagtt ttattcaatt tcataaatta taatacttca attataaaac
    15181 atttctatcc aacaagcata aatataaagt gggcacatat acctgcacat aaaccaccta
    15241 tagtatgcga aagtatcgct ttaccagcca atggtcttgc atctaaagca tccatcatac
    15301 ctatgtgtgt acttaaatat ccactccaac acatacccat tgctgtgaat actgctatat
    15361 cattaggagt tatagcgcct gattttataa actctggtac taaagatatt gctgctccaa
    15421 cagctcctaa tgcagttaca gggaatgcta ttgcctctgg tgatgtgaat ccaaataatg
    15481 gttctattat gaagctaatc ttatcaccaa tagcaggtaa taattttata ccttcatatg
    15541 cagctcctgt gtaaacagct tgacctgtag cagggtctgt agatggtcca aaagttaata
    15601 acataactaa tgtacaaact actaaaactc ctggtattat agccataccc atttcaacac
    15661 ctaacttacc accttcaagt atagcatcca atgttctttg gaatacatta ccatctctta
    15721 tttctctaaa ctcttcaagt tttgcacctt tatcatttat ttgcttttca gtatcatttt
    15781 tagggtcata attataatac tttttagtaa aagtcaacat aattctaaca ctaataatac
    15841 tacctattat tgccgcaaca ttacctatta tagctggtag tacatattct ttaccttgag
    15901 ctatcataaa agttgttaca ataagtccca taccaaaggc tgttcctaaa ttacataaag
    15961 caggtacttg atacttctta aaatattgtg taaatgtttt gtctttagca aatggtataa
    16021 tagctggatt atcagataaa tatgttgcaa ctactcctgc tatactagca ccaggtaagc
    16081 cccatatagg tctcataaga cccttaaata tcttattaac taaagatatt actccaaact
    16141 ctgaaagcaa tgcactaagt gcacctgcaa gaactgccat agccattatt aaaaatactg
    16201 tgtctaataa aagcgcatgt gctgtactca ttattacttt aaacatcatt cctgcaccca
    16261 tgatgcttcc tacatatcca aatcctaccg ccaatagtac aataaatacg aaggtttcca
    16321 tacttactgc cttgactctt ctcttatccg acagcttttc catcttgaaa tcttccccct
    16381 tttaatttta aattaaggtt cctaacattg gtacaaattt tttggtacaa actgagttct
    16441 aaaacttaca ctcgtaatgc aagttgtcag ttttttgaaa taaaaaaaaa ttacgtttat
    16501 agaataagaa tatcctcttt aatttggata ctcttattct attttatgtt attatattat
    16561 catccttttg tttaaaagtc aattttttct atttttgttg tttaatttta tttatactct
    16621 ttttatattt ttcattttct tttatctttg taagtactcc atataagttg acttttctaa
    16681 atacagtagt tataaatacc aatcctaatg caattgaact tgcacacgat aatgtactca
    16741 ccccatattc tacagctttt atataattat tggtcaaaac agaatacata gtattatata
    16801 ttccataacc aggtactaat attattaaac agcaaacact taatgtagtt gctggagtca
    16861 tataaattct tgcacataac tcacaatata tcgcaaaaca aactgtagct ataaaaaaac
    16921 ataagttttc tgttactccc atatttaaaa gaaatttata cgaaaaccaa gctatacttc
    16981 cacctattcc tgctagtatt aagttttttc ctttcatatt aaatagcact ccaaagctta
    17041 aggcagtaaa aaaagctgct attacttcta taaacaaatg cacttatatc ccccctaact
    17101 tgaagtacaa tccaagtacc aagaacattc ctactgctat agatacacct acaaaaaata
    17161 cttctgctaa ttttgcaaga ccagaaatca actgtccatc taacaaatct cttaatgcat
    17221 tagtcaaagc aagcccagga actaagagca ttattgctcc tgctattaaa tggtctaacg
    17281 tctttatgaa tccgagcttt aaaattaaaa ttgaacaaga agctataata actgctccaa
    17341 ttgcatttac aaaaaagtca ttaagggaag atttacttaa atacataata acaaacttag
    17401 tcagaatccc aacagtaaat gcagctataa attcttccac atttcctcca aataatatcg
    17461 aaaaagttgc tgctgcaata cctgcaaaaa atattgttat taaatccgag taatagttat
    17521 cttcacttat ttcatctaaa attttctcac ataattcaat atctaaatta tttatactta
    17581 ttgctcttga taatgagtta acttcatgaa ccaagtttag gtctactctt cttgagctta
    17641 ttcttttaat tgctgaatgt aatttcccat ctacaaatac agatgccatt acagctgttg
    17701 gagttgcaaa tacatctacg tggtctattc caaaagattg acatattcta gctatcgttt
    17761 cttcaactct ataggtttct cctccacttt gcaacatagc tttacctgca tttgaggaaa
    17821 agtttaaaat tctatctata tccacaaaac atatctccca ttcttttata gtattattca
    17881 tattttaata caagatatta tttatttaaa ctattttaaa taattcatat ccaatattta
    17941 cattttatga aaaaacttgt tttttatgct tgtcaaaatc aaattttaag cttgtttaca
    18001 ccataaaatg gctgaattaa tatttgacaa acactttcaa ccatttatat aaccttctaa
    18061 attgtataac tttttactac aaataaaatt atatctcttt taattctttc tcttgagtat
    18121 ttagcttata gcaacttcat atttatatat gtacaatata aaacattatt tttatgatac
    18181 aaaatataat ttgtaaatgt atatatgtat taactgtgta tatataaatt attcttaacc
    18241 ttatcagttt tttcatatat cttcatctat accgaccata tattaattac attgaccata
    18301 tattaataaa tgatattaca tagacttaag tacattaaaa aacttaaatt tagttattta
    18361 acaacataat actctaattt aatctataaa aatccatacc taatataatg caactcttat
    18421 tttaacacca tctattacac aaaacaacat attaaaaagt aaaatacaaa atatctacta
    18481 taaaccttat tttaatactg aatatatatt ataatatatt taatgtagct taaatatagt
    18541 ttaaacataa agaaaggaaa cggtatatgc taagaaaaga taaagttcta ttatctctac
    18601 ataaattatg tgaaaatatg actttaaaag accttcaatc agataaaaca ggattcacta
    18661 caatagaaat tgctaaccat tcatctcttg atagaagtaa taccagtaaa gaattaaata
    18721 acctatacaa tgaaaaaaaa gttctgaaaa tatctggtaa gccaatttta tttctacatg
    18781 ctagtattat agaaaattta attggccgta ctttgaacag tgatgaattt tctataaata
    18841 attgtaatga gctacttaaa tcaagtaaaa atgactcaga agatattttt tcaacattac
    18901 ttggacatga ttctagtctt aaactaccta tagaaaaagc taaatctgct atactctatc
    18961 ctccaaaagg tcttaatacg ttactcatag gacctactgg tgttggaaaa agtacatttg
    19021 ccgaaacaat gtacaaatat gctgtagaaa gtaaaatctt ttcttctgaa agcaaattcg
    19081 tagtatttaa ctgtgctgag tatgcagaaa atgctaactt attattaagt aatttatttg
    19141 gttatgtcaa aggttctttt actggtgcta ataaggataa attcggcata gttgcacagg
    19201 ctgatggagg catattattc ttagatgaaa tacacagatt accaccagaa ggtcaagaga
    19261 tgctttttct tttgatggac aaaggcatat acagacgatt aggagaatct gacattgtgc
    19321 ataaagctaa tgtctttatt gtgtgtgcca caacagaaga tatacattct tctctgcttg
    19381 ggactttttt aagaagaatc cctgttacaa tcaacctacc tgctttacag gataggtctt
    19441 taaaagaaag attatctctt attaaatact tctttattat agaatcaaaa tacaccaata
    19501 caactataaa agttcacaaa gatgtaatta aatcattact cctttatgat tgttttggta
    19561 atataggtca gcttaaatct gacatacaac ttatgagtgc aagagccttt ttaaattaca
    19621 aaactggact taaggataat atagaaatag atttatccct tgttcctgat tacatctata
    19681 ttggtctttt aaaattgaat gaaaatcgta gtaatctaaa taaattatca gaacttgatt
    19741 atatgctttt ttatgaattt aatggtaata acttagaagt tgaatataca aaagaactat
    19801 ctaaatacaa tatagataca tcaaacaatg gatttaaata ctctacagag attccttatc
    19861 tggaaaatga tataaggagt atgtttataa attacatgga aaagtactct acaaaattac
    19921 tttcgatatc ttcagtaact cattctgatt atacaagtaa tgagattttt aaagtaatca
    19981 atccaaagat ttactatgct gttgaacatt ctttaaaaga ggctgaaaaa atatcgagaa
    20041 acaaatacac aaaacaaact tatattgctc tttcaatgca tataagtgca cttatagaaa
    20101 atataaatca agacacatat aatcctaagt acaatttata taatgataaa attttcttag
    20161 ataaagattt aaatatagct aaaatagttt taagtataat tgaaaatgac ttacaaacat
    20221 ctttacctaa agatgaagta aaatttatat ccatgtttct aaatagcatg tatcttgaac
    20281 caaacaatca atctgaaagt ataggagtca taatacttgc tcatggaaat tcaacagcaa
    20341 gcagtatgtg tgatgtagca aattcattgc ttggtacaaa ccattgtaat gcaattgaca
    20401 tgcctcttga tgtaaaagta agtaccatac ttgataaaac tatagagttg gtaaaagaat
    20461 gtgacgaagg taagggtgta cttcttttaa cagatatggg ctctcttata gcttttggag
    20521 aacttatttc agaaaaaaca aaaatcaaaa ctcgctcaat tgaaatggta tctactccaa
    20581 tagttttaga agcagtaaga aaaagtacac taccagaaat gactttggat gaattagcta
    20641 attccttaag ctctttaaat ccatatattg gacgagcagc aatcgattca acagaaataa
    20701 actatcctga aaagtcaaga gagtatgtaa taataactac ttgtataaca ggatatggtt
    20761 cagctatgaa agtagcagac tttataaatt cttcacttaa aaatattagt aaatacaata
    20821 ttaatttagt tcctcataat atagaaagtt ttaaaatgta taagaataaa tttagtaata
    20881 ctaaaattct ggcagttgtt ggaagtgtag attttaatgt agccaacacc ccttttatac
    20941 acatagagga actactaaat ggatatggcc tagatatgct aaacaaaata atagaaggta
    21001 cattcaatgc caatagtatt acaaatataa aagaaacaaa tctatcatac aacagtttaa
    21061 taataaactt tatgacccaa aacttagagc ttttggactc tgttaaatta ttcaactatg
    21121 tcaattcttc cttatttaca ataatagaat taatagatga aaacttaaag agcaaattta
    21181 agctaggata tatactacat tgctctttta tgatagaaag atgttttaaa aatacaactt
    21241 ttccatataa taacattact gaattaatta gtaaaaatca tgaactatat agtttaatac
    21301 gtgaagcctt taatccaaca gaagcttatt ttaatatttt gataccagac gaagaaattg
    21361 cttatataat agacatgatt aatgagtatc taaataaata aaatttatag cacttatttt
    21421 gtgcatatta attaatatat gaaatggagg acgagatttt gaaaggaaaa ataaaacagt
    21481 taacaatact tgcattaatt tttatattta taactcctgt atttgcattt gcagatactc
    21541 caccagtacc aaattcttca agagcagcac tacttataga ccaggaaact aaaagaatat
    21601 tatttgaaaa aaacattgat gaaaaaatgc cattagcgag tttaagtaaa atgatgacct
    21661 ttttattggc aatagaagca gttgataaaa atcaagttaa agagactgat atggtgaaaa
    21721 tagacaagtc tacagcttca gtaggaggtt ctacttgtaa acttaaagat ggtgatgaaa
    21781 tatctctagg agagcttatg cagggtctaa tgctagtatc tggaaatgat gctgctatag
    21841 ccatagctaa acatattggt aaaactgaaa agaattttgt aaatatgatg aacaagaaag
    21901 cagaagaaat aggaatgatt gatacgtatt attttaatcc aaatgggctt cctatatata
    21961 ctgacccaga acataaagaa cctcctattg aaaatatgtc aactgctcac gatatagtta
    22021 cacttggaaa gtatatgtac gaccattatg aaaatcaagt tacaagaata accactatgc
    22081 aagtatataa tgatactaaa aaggatttta cacattataa cacaaatccc ctacttgttt
    22141 cagtacctgg tgttgatgga ataaagacag gctatacaga taatgctgga tattgccttg
    22201 cattttctat gatggtacct aaagatgcta aaaacgaaag aagtcataga ttgataggtg
    22261 ttgtacttgg tgatggtaat aagaaaaata gaatttcttc ttctgctagt ttattgaagt
    22321 atggtaaaga taatttccat tctaaaaaaa tagcacataa gggtgatatt atagagactc
    22381 cttgtgttga tggtattgat gattttaaga taacagttaa ggttgataaa gatttgtatg
    22441 gtgttgtttc agataacgag aatatcaatc ctaaggtagt atttaaaaat atgaattatc
    22501 ctatccataa gggagatatt gttggtgttg caaagtatta taatgattct ggtaaatttg
    22561 ttggaagtgt tgatgtaaaa agtgaaagta atataggatg tattcctttg aaggataaga
    22621 ttaagattaa ggttgctaag ataaataaaa aacttgagat taaaaattct gtttgtttta
    22681 aagtatagta aagcatacta ttaaaagggt atgttaaatt tataaagact ccactttgtg
    22741 gagttttttt gttttttaca aaaattaaaa tagtggcaac aattgaggat gtgagggaga
    22801 gttgccacta ttttgtatag ggtattatat tatgttttta agctagtttt actatcttct
    22861 atgtttatat aataactctt gtgtgtgaat ttaattgata attaatatga aaattatgtg
    22921 aagttttatg tgaatcttat tataaacata ctaaaagagc tgtaggtaag tttactttta
    22981 tgcaaattta attacagctc ctttcattat atattaattt tatttactaa aattaaaaat
    23041 atttaatgct attctaccta atagagatgt tggtattgat actgtctttg ctggtactgt
    23101 tacatctatc tctgctgtat ctgctgtttt tgaacttgct gtatgtgtta ctgtaaatac
    23161 tagttttact gttttttctg catctggtgg cactattgtt ccatctgtct ttattacatt
    23221 ctcatcactt gatgttttta ttgctatttc ataccctgct gctactgttg gcatagtcaa
    23281 cttagttgca tcttttaatg gtgcagttac acttgttatt tttgctgcct ctgcttttac
    23341 taattcttca tctgttgatt tagctggtgc tgttacagat agttcttttg tatctgctgt
    23401 tttaccactt gatgtatgtg ttaccgtaaa tactaatttt actgttgtta ctgtatttgg
    23461 aggtattatt gttccatctt tttttataac gctctcatta cttgatgttt ttattgctat
    23521 agtatatcca cttgatactg atggcatagt taatttagtt gcatttttta ctggtgcagg
    23581 aacgcttgtt atttttgctg cttcattatt tactgatgtt tgattagaag aacttcctcc
    23641 tgaagatgag cctcctccat gtgaagaacc tccacttgat gaacttcctc ctgaagagtt
    23701 agaacttgga ctatttccac ttattgttgg agaattatct cccttaacac tacttattgt
    23761 acctttatta tctatagtag tccctttaga attatttgta actgtagtta tcttaccacc
    23821 attagtcact tttacttgac ttgctccaga tgctactgtt aaactaccaa tctcaccttt
    23881 agaattattc tctatcttag ctccattttt atcattaacc tttaagttag ttatttttcc
    23941 atagttgaca aaagttccat cttttacatc atctataact acatcatcca cttttgcata
    24001 gttatttaca tccccattag gcatgttgat agtcacagtc tttgtatatg taccatttaa
    24061 gtttactgtt atcgccttat cagtttgtat tgtaaatgct tctttaacct cactagttgg
    24121 cttaaagttt ataacatcat tagcagtagc tttatctata gctgatttta attcagaaat
    24181 tgttttggct tcaactgttt ttacttcttg taatgacttc atttcattaa atgctgtctc
    24241 attaccattc ccaccaactt tagttatctt atcaaaactc ttagagttaa ctagagattt
    24301 ttgtgaagct gataagttct ttccaactaa aactactggt gattgatttt tagcacctaa
    24361 tacacctact gctagtgcat ctataagttg gtcttgttta ttcataccat ctttagttac
    24421 ataagcattt tttaaatctg tctttttata aaattctttt attacttttg catttgtatc
    24481 atttctatct gttccaccta gtcttgtact atttggtagt ttatttttaa cagcttcaga
    24541 aattgaactc tctccaccta ctacataaga ttgagttatt ccagcatctt ttataaattt
    24601 gtctgcaact gctgttccat ttttagaatc agcaagtatt actggcatat tgttttgtgc
    24661 agctggtgct cctacactta cagcatctgc aagtcctttt tcaccattaa ctacagctat
    24721 tttcttaaca tcacttatag catctaattt ctttgccaat tctaaggatg tttgatatct
    24781 ctcagctcca gagattcttt caaaagatat ttttaaatct tttatttctt tttctatatt
    24841 tgtacttaat acagaagttc cacctattaa gtatactttt ttagctttta atctttgtat
    24901 ttctgctttc gtcttatcat ttaatttatc tttacttgtt aaaagtattg gagcattttt
    24961 tgcttttgca aatggtgttg cagatagagc atctgctata gatgagtcgt ttacgattac
    25021 tacctcatca gcatttttcc atccatcttg acttatttta acagcagttt catatctttc
    25081 ttgccctgtt aaatttgttt ccacagtttg tgcactagct atgcctgctg ttgaacttcc
    25141 agctatcatt gtaactgtaa gcattgcagc aatcttcttt ggtattttca ttttattttc
    25201 ttcccccttg tccatttgat attacttaat tttttaactt tgttcccgtt ctaattctaa
    25261 aggatactta atatctaatt ttaaagaggt ttctaactat aatgatttca taattaattt
    25321 gtaattattt tgttacatta cagtcattat gtaaggaata aagaaatttt cttattgatt
    25381 gaaaaatttg taaatttata ttttaaggta agtttgattt ttatgttaat gaattgttat
    25441 aaaaaacatg gttttgtata aaaaaagt
//
LOCUS       DAELJO010000035        25333 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELJO010000035 DAELJO010000000
VERSION     DAELJO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.035"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(35..1114)
                     /locus_tag="KPB88_001283"
     CDS             complement(35..1114)
                     /locus_tag="KPB88_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0053579.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      1402..1517
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1692..3062
                     /locus_tag="KPB88_001284"
     CDS             1692..3062
                     /locus_tag="KPB88_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0053580.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(3327..3938)
                     /locus_tag="KPB88_001285"
     CDS             complement(3327..3938)
                     /locus_tag="KPB88_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG0053581.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(3928..6291)
                     /gene="lon"
                     /locus_tag="KPB88_001286"
     CDS             complement(3928..6291)
                     /gene="lon"
                     /locus_tag="KPB88_001286"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG0053582.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            6471..6983
                     /locus_tag="KPB88_001287"
     CDS             6471..6983
                     /locus_tag="KPB88_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0053583.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            6971..7498
                     /locus_tag="KPB88_001288"
     CDS             6971..7498
                     /locus_tag="KPB88_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0053584.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(7569..8819)
                     /gene="clpX"
                     /locus_tag="KPB88_001289"
     CDS             complement(7569..8819)
                     /gene="clpX"
                     /locus_tag="KPB88_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG0053585.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(8838..9422)
                     /gene="clpP"
                     /locus_tag="KPB88_001290"
     CDS             complement(8838..9422)
                     /gene="clpP"
                     /locus_tag="KPB88_001290"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0053586.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(9572..10858)
                     /locus_tag="KPB88_001291"
     CDS             complement(9572..10858)
                     /locus_tag="KPB88_001291"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG0053587.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(11052..11522)
                     /locus_tag="KPB88_001292"
     CDS             complement(11052..11522)
                     /locus_tag="KPB88_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0053588.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(11522..12871)
                     /gene="rph"
                     /locus_tag="KPB88_001293"
     CDS             complement(11522..12871)
                     /gene="rph"
                     /locus_tag="KPB88_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG0053589.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGNGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(13031..15055)
                     /gene="ligA"
                     /locus_tag="KPB88_001294"
     CDS             complement(13031..15055)
                     /gene="ligA"
                     /locus_tag="KPB88_001294"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG0053590.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(15241..16179)
                     /locus_tag="KPB88_001295"
     CDS             complement(15241..16179)
                     /locus_tag="KPB88_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG0053591.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNRERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(16281..16703)
                     /locus_tag="KPB88_001296"
     CDS             complement(16281..16703)
                     /locus_tag="KPB88_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG0053592.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWKLNNLIENLVQERNAMSLGKTIEHK"
     gene            16891..18174
                     /locus_tag="KPB88_001297"
     CDS             16891..18174
                     /locus_tag="KPB88_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG0053593.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(18252..18767)
                     /locus_tag="KPB88_001298"
     CDS             complement(18252..18767)
                     /locus_tag="KPB88_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0053594.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(18767..20143)
                     /locus_tag="KPB88_001299"
     CDS             complement(18767..20143)
                     /locus_tag="KPB88_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0053595.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(20168..20719)
                     /locus_tag="KPB88_001300"
     CDS             complement(20168..20719)
                     /locus_tag="KPB88_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0053596.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(20777..20950)
                     /locus_tag="KPB88_001301"
     CDS             complement(20777..20950)
                     /locus_tag="KPB88_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053597.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(20961..21794)
                     /gene="fdhD"
                     /locus_tag="KPB88_001302"
     CDS             complement(20961..21794)
                     /gene="fdhD"
                     /locus_tag="KPB88_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG0053598.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(21843..23987)
                     /gene="fdhF"
                     /locus_tag="KPB88_001303"
     CDS             complement(21843..23987)
                     /gene="fdhF"
                     /locus_tag="KPB88_001303"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23571..23573),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG0053599.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(24238..25296)
                     /locus_tag="KPB88_001304"
     CDS             complement(24238..25296)
                     /locus_tag="KPB88_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0053600.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         7511 a         4662 c         2861 g        10299 t
ORIGIN      
        1 tatcattatt aacattaatt ttaaacatat acgtttataa gttttcctca tattttaaaa
       61 ttcttttaat taatttatct cttaatttcc ctacttcatc aaatctcata ttaggactat
      121 agtaactttc tataatatca tgtttagact tggctctctt aagatttagt actgcccaac
      181 aaattaaatc atctaaaact ttttcatcaa atttaatatc ttcttcatat ttaagtagtt
      241 tttctctatt taaaaactga tttaaatcaa ttgtatcttc ttctttaaaa gtcgtactta
      301 tagttatgcc tatatctaaa tcagttaata gtacagtttc aattttctct ggaataagtg
      361 gataatgata aacttctaca tttaaacctt tttcaatagc tttcttatat actttgttta
      421 ataatgtagt tttcccagtt cctatttctc catctaaata atagactttc tttacatctt
      481 ttaaaatgtt ttctgtataa tctaaacacc ctacaggagt tatagctgaa ccaaataaat
      541 gcttttctct ttttagattt cctgtgcttt taactccatc aaataacctt tttataaatt
      601 cttctgtgac caaatttact ttgccataat ccatacaatc agaatatttt tcttctatgt
      661 ctctaaagat tggttctgct gattttaaaa acttaaatgc tcttttaaat gaattactaa
      721 tatctttatc ataaactgat atttcttgtt tattcttttc taattcttct gtattccagt
      781 aatcacctaa attaattatc tcatccaaag ctccaggtag ctttgggtcc atagtatgtg
      841 gggcagttgc atccataagt accacgccta atttctttat aactaaacca tccaacgaac
      901 ttgggtcaga aggacaatga tataaatcaa tatcatatcc tctatgcaaa aactctttat
      961 aaattttttt cataagtgag gattttccta ctccaggtcc accctttaaa caaaaaactc
     1021 tttttacatt ttcaggaatt atatagtcaa aaaaagaata tgagccttgt gatgtatttc
     1081 cacccgggaa tgccttcttt acctttcctt ccatattagc gcctcctcat aatttacaat
     1141 atctcttaat taagatattc atattataag aattttatta cagctttctg attttgataa
     1201 atttaaaact taccatctaa tactaataac aggttaattt tatctaatta ttatagactc
     1261 tcatactgtg ccttattcta tctataaata caatttaagt gtccatattg aaatatttgt
     1321 attgtaatac agctggatat tacttaaatc caattgtttc cattataatt ttatgttaaa
     1381 ataatattac aaaatacatc tgtttttctt cataaacggg tgaaattccc tatcggcggt
     1441 aaaagcccgc gagccttatg gcataatttg gtcatattcc aaagccgaca gtaaaatctg
     1501 gatggtagaa gaaaatagta tatgagtacc tttatgtaat tttacatgag taatctatac
     1561 aaatccttca actaccgtat ttattcatga aattagacac attcaaggta cctaatatac
     1621 aggtgctttt tttgttgttt attttacaat tatatcgtac ttataaaatc tattaagatt
     1681 ggagtgttat catgaaacaa aaatggatag tattgattat catctgtatt ggtgtattta
     1741 tgtctactct tgatggaagt atactaaata tcgcaaatcc tacaattgct gctgacttta
     1801 aaattaacat gtcacaaatt cagtgggttg taacagctta tatgcttgtt gtaacagcta
     1861 ctatgttatt tttcggaaaa cttggcgata aagtaggttc aaatcgtcta tacacattag
     1921 gattttttat atttacaata ggttcttttc tgtgtagtat gtcaaacaat ttatcaacat
     1981 taatttcttc aagaatcttt caggcagttg gagcaagtat tttaatggct actgggcttg
     2041 gtatagtttc caatgcattt ccagcaaatg aaaaaggaaa agctatagga atcactggag
     2101 ctgtagttgg tataggaaat atgagtggtc ctgtaattgg aggtataatt ttagaacatt
     2161 ttggatggcc ttctattttt attatcaata tacctattgg tattatagca gtctttcttg
     2221 gcattaaatt tctgcctaaa cctgttttgg atgaacaaaa caagagcttt gatattccag
     2281 gtcttctact tttcgcatct tgcactacgc ttatattact tgcaatgaat gagaagggta
     2341 atacaagatt gtatttaggt ataacagcac ttataatctt cttattgttg gcattaagag
     2401 aagtaaaatt tgaacaaagc tttattgacc tacctctatt taaaaataga aactttactg
     2461 taggcaatat cattggagta gcttgttatt ttcctcaaat ggcagtatct tttctacttc
     2521 cattttattt agaacaatta aaaaatttat ctcctatgat ggctggttat gtaatgactg
     2581 tacatccact tattatggta cttatagcac ctattgcagg ttcactttct gataaacacg
     2641 gtgcaaaaaa cattctaaca gcatcttttt catttatgac catctcacta gttggaatgg
     2701 cacttttaaa agcagattct ccattgtatc ttttaattgt atgtttagtt atttttggac
     2761 ttgggctagg agcatttagt tcaccaaaca atagtagtat tttagcagat gttcctcctc
     2821 aaaaacaggg atacggaggc agttttcttg caactattcg taatttatca tttgctcttg
     2881 gaacagcatt ttttagtagt ttttttgcac aaagtcttac atacaatcaa aagtttaaaa
     2941 gtcacacatc agcctatgtt atagctagca atcagtctta ttggattgca gcatcagtat
     3001 gttttatagg gcttatacta actgttttct ttatgagaaa aacagataaa tctatttctt
     3061 aaatgctatt tattgctgac tacataagtc aataattttt tagttgaact aatttatact
     3121 aattttccca taccttacta aaatttatta gtatttagca aaattactga tatttattgt
     3181 ttataaacaa taaggatgcc aaaaaaagtt cattaaaact attttaaggc atcctatatt
     3241 ttgctttaat attaatataa ttaaaactaa ctattattac atttattatt caaataaatc
     3301 ttatgtcaat atttaacact ttaaatttaa tctactgtta tttcatatcc atgttgattg
     3361 tattgattta caatctcttc ccataattct tcaacaccag tcttttttag tgaagaaaca
     3421 ggaattactt tttcactact ttccatttgc aatttttttc ttattatact tatgtgtttt
     3481 tgatattgtc ctctagatat tttatctgcc ttagttgcaa taactacaca gttatatcca
     3541 aagtgcttta tccattcata cataagctta tcatctgctg taggctcatg tctaatatct
     3601 acaagtagga atatagaaca taactcttgt ctactttcta gatatcgttc cattatacct
     3661 ccccactttt ctttttcaga cttagctatc tttgcatatc catagcctgg aaggtctaca
     3721 aaataaaatt cattatttat tagataaaaa tttatagttc tagtttttcc tggtgtttga
     3781 ctggttctag caaagtttct tctgttaagc aatgtattta ttattgatga ttttccaaca
     3841 tttgacctac cagccaatgc tatctctggg attccttctg ctggatactg tgatttattc
     3901 acagcactca tagttatctc tgaacttcta attttcatta ttttcaccac tctttaataa
     3961 agcttgttct aatacttcat ccatatgttc tacaagtaca aactccattt tttcttttac
     4021 attttctggt atttcatcta aatcagcttc acattcttta ggaattaata cttttgttat
     4081 accagctcta tgagctgcta ataacttttc tttaactcct cctactgcta agactctacc
     4141 tcttaaagtt atctctcctg tcattgctat atttccaggc acaggtcttt tagtaagtgc
     4201 tgagattact gcaagagcca tagttatacc agctgatggt ccatcttttg gaacagcacc
     4261 ttctggaatg tgtatatgaa tatcattcgt cttataaaat tcagggtcta tatcaaactt
     4321 gtccactatt gaccttatat aagagatacc tgtcttcgca gattccttca taacatctcc
     4381 taattttcca gttaatacaa tttcgccttt acctttaagt acgtttactt ctacttcaag
     4441 tgtaactcca ccaacttctg tccacgcaag accatttact aatcctattt gagggttaac
     4501 ttctgcaagt tgatacttaa acatgtcttt tccaagatat gtctctaggt cagacttatt
     4561 tataacaacc tcttcaagag ttgggtcttc aacgtatttt ttggcaacct ttctacaaat
     4621 cttaccaata gttctttcta gtgttcttac acctgcttct cttgtataat ggttgattat
     4681 acttcttaaa gtttcatcat ctatttttat aaagttttct tttaaagcat gttctttaat
     4741 ctgttttgga agtaggtatt tttttgctat atttaatttt tcttcttcta tatatccaga
     4801 tacttctatt acttccattc tatctaataa tggtcttggt atattaccta gactatttgc
     4861 tgttgtaaca aatagtattt ttgataaatc aaaaggtatt tctaagtaat ggtccacaaa
     4921 atccttattt tgctctgggt ctaatacttc tagcattgct gaagctgggt cacctttata
     4981 atctgctgcc atcttatcta tctcatcaaa taagaataca ggattttttg tttgggcttc
     5041 ttttacccca ttaattattc ttcctggaat tgaacctaca taagttcttc tatgacctct
     5101 tatttctgct tcatctctaa ctccacccaa agatattctt acaaattttc tatttaatgc
     5161 tctggcgatt gattttacta tagatgtctt tcctgttcct ggaggaccaa ctagacatat
     5221 tataggccct ttaagtgact tagcaagagt tcttatagct agatactcta gtattctctc
     5281 cttaactttt tctaatccat aatggtcttc atctaatata tcttttgcct tagttatatc
     5341 taatttatct tttgtctctt tattccatgg caatgagaat atagtatcta aatagtttct
     5401 acttacagat acatctggag acatagaaga tatttttgaa aatttatcta tctctttttc
     5461 tattttctct ttagttgtct taggtgcttt tattttttta agcttttctc tatactcatc
     5521 tgcttcagag tttatatctt cttcttcacc taattctttc tgtatagctt ttaattgttc
     5581 tctaagataa tattcttttt gaaccttatt catctgtttt tttactctta aagttatttt
     5641 cttttctatt tttaatatat ctatttcctc taataatata gaataaataa gttcaagtct
     5701 ttttctaata tcaaattctt ctaatatttc ttgtttttgg cttgatttta gatatatgtt
     5761 tgcagctatc gtatctataa atctatctac atcttctata tcagctaaag atattaatat
     5821 ttctggtgat accctatttc caatatttat atactcttca aaggcatcaa atacatttct
     5881 tacaaatgct tcaatttcaa cttctgtttc actatccaaa ttatcactat caaatactat
     5941 ttcttctatt actgctctga aatacccatc ttcttgttct attttcttta ctctacccct
     6001 tgatacacct tcaaccaaaa cacgaactgt atctcctggt aattttatca tttgcttaac
     6061 tttacagatt gttcccacat gataaaaatc ctcttctcct ggctcatcta cctctgcttc
     6121 tttctgagaa gttaaaaata taagttcttc atccatcata gcttggtcta aagctttaag
     6181 agatatctct ctacctatat caaaatttaa tatcatatat ggaaatatag ctaatcctct
     6241 tagaggaatt aatggtaatt catgatctat tttagtataa ttttgttcca tcaacattac
     6301 tccttttcta gtctaattgc tttgctttta atattatata tttgaacaag ttaattttca
     6361 acattaatga taaattctta gaatactatc atactatatt atctttttat tatgatataa
     6421 tattaatatt agtatactta gatgttattt atcttttgga ggtttctatt atgttgaaat
     6481 tgtttttaac ctttttaaaa attggtgcgt ttacatttgg tggtggatac gctatggttc
     6541 ctcttataca agatgaactt gtaaacaaac aaaaactttt agagcaggaa gaatttattg
     6601 attatttatc catagctcaa agttatcctg gagtactggc agttaatata tctgtattat
     6661 taggatataa actttatggt ataccaggag ttcttatatg taccttaggc tctgctttac
     6721 catcattttg tatagtctta gtcttatcgt atctatactt taaaaattca tcatctaaaa
     6781 ttttagatgg attttttaaa ggagttgcgc ctgttgtaat agctcttata ttgtattctt
     6841 ttacgagcat gttcaagaaa ctaccaaagt ctaaaactaa catactacta ttaattatag
     6901 cagttttatc tgttggtgta ttcaatataa gccctattta tttaataatt ggaggaggtg
     6961 tatatagtct atgtcagaaa taatactact cttcttaata ttccttaaaa taggggcatt
     7021 tagttttggt ggcggttatg ccatgctacc atttatccag caagaattta tatctaaata
     7081 tcactttata actaatcaag agtttttgga tttacttgct atatcacaat ctacaccagg
     7141 tcctgtagca ataaatagtg ctacatttgt aggatttaag actgctggct tctttggttc
     7201 tctagctgca actattggtg ttacaatttt tgcattaata ggtctaacta ttatatcaaa
     7261 gttattttct aaatttaaaa ataataaaaa ggtagaaaac ttattgacag ttcttagacc
     7321 aattactcta ggattcatat tatcagcagc tttttcatct gccaaagaag ttaattggga
     7381 cttatatgga ataattgcat ttatagcatc attttatctt ttatataaag gaaaaattgg
     7441 ttccattgca attgtattcg tctttggtat tctaggtata ttactcacat ttttataata
     7501 atgataccta ttttatattt agcatattaa cataaaaaaa ggagctttta aagctccttt
     7561 tcatcatctt atgcactttc ctcttgatcc ttaagtacta tgataggatt tactgagtct
     7621 tcgttcacag atttctctgt tactataacc ttctttatat tatctctaga tggtacttca
     7681 aacatagttt ccatcattac gctttcaact atacttctaa gtcctctagc tcctgtattt
     7741 ctttctatgg cctttttagc gattgcacga agagcacctt cttcaaattc tagctcaaca
     7801 tcatctaact ccaataactt tttatactgt tttactagag catttttagg ttcctgtaat
     7861 atctgcatta atgcatcttc atccaacaac tctaaagtag ctaaaactgg tattcttcct
     7921 ataaactctg gtattatacc atattttaat aaatcctctg gaagtacttt ttcatatagc
     7981 ttccctaaat ctaattcttt tttactttct attttagctc caaaacctag tgttttatct
     8041 ccaccacgtt tttgaattat cttctctaat ccatcaaaag ctccacctaa tataaataat
     8101 acattagtag tatctatctt taaaaattct tgatgaggat gttttcttcc tccttgtggt
     8161 ggaacatttg caacagtacc ttctaatatt tttagaagtg cttgttgaac accttcacca
     8221 ctaacatctc tagttataga tgggttttca gattttcttg caatcttatc tatctcatct
     8281 atatatatta tacctctttc agctttttct atatcaaaat cagcagcttg aataagtttt
     8341 aaaagtatat tttcaacgtc ttctccaaca tatccagctt cagtcaatga tgtagcatct
     8401 gccattgcaa aaggtacatt tagagttctt gctagagttt gagcaagtaa tgtctttcca
     8461 gaacctgtag gtcctaataa aagtatatta ctcttttgaa tttctatatc cttactacta
     8521 gattttttac tgtatattct cttataatga ttataaaccg caactgataa agctttttta
     8581 gctttttctt gacctattac ataatcattt agaatctcca tcatttcctt tggttttggt
     8641 aaagatgtag tatcctcatc tatagtatct tctatctctt cttgaataat ttcgtcacaa
     8701 agttcaacac actcgtcaca aatatataca tttggacctg caattaatct tcttacttga
     8761 tcttgatttt taccgcagaa tgaacacttt aattgtcgtt tttcttcata tttagacata
     8821 ttaacacctc tctctatcta cggtctcttt gtaaatactt catctattaa accatattct
     8881 ttggcttcta gtgcactcat gaagttatca cgttcagtat ccattttaat tttttctaat
     8941 ggctgaccag ttctttcaga taatatctca tttaaagttt cttttatttt taaaattctc
     9001 tttgcatgaa tttctatatc tgttgcttga ccttgagctc cacctaatgg ttgatgtatc
     9061 attatttcac tatttggtag tgctagtctt tttccttttg ctcctgctgc caacaagaat
     9121 gctcccatag aagcagccat acctatacat atagtactta catctggttt gatgtattgc
     9181 atagtatcat atatagccat tccagaagtt atacttcctc ctggagaatt tatatataaa
     9241 tgtatatctt tgtctgggtc ttcagcttct aaaaataata gctgtgctac tataagccct
     9301 gctgtggcat cgttaacttg atctcctaag aagattattc tgtcttttaa tagtctagaa
     9361 aaaatatcat aagatctttc tcctcttcct gtttgttcaa caactacagg tactaatgcc
     9421 atggtttcat acccccttta ttctatataa tttacagtag ctttttttaa gctactgtaa
     9481 attattatac gcatttaact aaattatatt tgcacatcat tatatactaa acatttcata
     9541 acgtcaacta tattagttag cgtttatttt attaagctat tgttgcatta tcaactaata
     9601 aatctattgt ttttcttatt tttatttgac cttttatatc ttctttgtca gcatctctta
     9661 aagaagcttc tattttttct acttccatat tgtaagcaga agccattttt tctaactcag
     9721 ccttaaattc ttcttctgtt gcttcaattc cttcagcttt agttattgct tctaatacta
     9781 aagaagactt aactaatttt ttagcatctt cttttctctc ttctcttaat tcttccatag
     9841 ttctaccagt catctctagt aattgttgta atccaaatcc ttgatattgt aattggtagt
     9901 taagttcatt taacatatta tctatttgat gttgtatcat aacttctggt atttctattt
     9961 ctgtattttc agcaactttt tctactactg aatttctagt ttctgcatct gctctatttt
    10021 tagcttcttc ttctaactta gctcttacat ctgcttttaa ttcatctaaa gaatcaaatt
    10081 cactagtatc tttagcaaat tcatcatcaa gtgcagataa ttcttttact tttacatcat
    10141 ttatttttac attaaataca actggttttc cagctaaatc ttgtgagtga tattcttctg
    10201 ggaaagtaac attaacttct acttcttcac cagctttttt acctactaat tgttcttcaa
    10261 atcctggtat aaatgtgttt gaacctataa ctaagttata attttcacct ttaccaccat
    10321 caaaagctac accattttca aaaccttcga agtctattat agctgtatct ccatcttcta
    10381 aagctttgtc ttctacactt actaatctgg catttttttc aaccatttct tgtagtttag
    10441 catcaacatt ttcgtcactt acagtattct caaccttagc tatttctata cccttgtagt
    10501 ttcctaattc aaactctggt ttaacttcta cattaagaac cataaccaac ccattgtctt
    10561 tacttatttc ttctatatct aaatctggat tgtctattgg gtctatgtct aattccttga
    10621 tagcttctgg ataaacttct gggaataata tttctatagc atcattgtag aatatacctt
    10681 ttccatattg agtctctata acaattcttg gagctttacc ttttctaaat ccaggtatat
    10741 tgtatttatt tctgctttta ttataagcct tatttactgc tgcttcaaat ttattgttat
    10801 ctactgttat ttttaaagta actttattac cctctttttt aattagttct gctttcatcc
    10861 ttctaatcct cctagtgata tttatgtaat acccgataac ggtattataa ttcattctat
    10921 ttataattca accacttaat tatatcacat aagttttttt atttacaagt gttccattaa
    10981 tatcgttgta cttatttgat aaaatttaat tttttgagag ttttttgcca tttttttaga
    11041 taaatacacc tttatatgcg tatttcttca atttttatgt catcttcttc tatatccata
    11101 atgacaaaac ttttatacga tactcctctt ggaagtgaag tgcttccagg atttatgtaa
    11161 agtacaccat ctttaatttc tctaaatggg gtatgggtat ggccaaatat aactatatca
    11221 gcatcaactt cagttgcctt tttttctaac atattagttc catattttac accatactta
    11281 tctccatggc ataagaatat ggttttattt gccacttcaa agacaacttc ttcctcaaca
    11341 gcatcaaagt cacaattacc tttaactgca ataataccta cattagtcat gctatgaata
    11401 tatcttgaat cagtaaagtt atctcctgca tgaattatta ggtcacattc tttcaaatat
    11461 ggtattgttt tttccatatt tttaatcatc atatgtgtgt cgcttactat gcctattttc
    11521 attacttcac tacccttttt ataaactctt gtttcataag ttttagtgcg tttgctctat
    11581 gactgattga attttttata acactaggta tttcaccaaa agtcttatca tatcctttta
    11641 ctataaataa gctatcatat ccaaaaccat ttttaccttt ttcttcaaat cctatcattc
    11701 cttcacaaat tcctcttact acaaattctt ttccatcagg aaaaactact gctattgcag
    11761 agacgaatct tgcatttctt ttgctcattg gtacattttg catagctttt aacagttttt
    11821 ttctattttc ttcatctgtt gcatgttctc ctgcatatct agctgaataa acaccaggtt
    11881 ttttgcctaa agcatctact tctaagccag aatcatctga tattgctata agtttagtct
    11941 tttttgctat tgttctagct tttatcaacg cattatgctc aaatgttttt ccatcttcta
    12001 ctatctctat acctgctaaa tcaacatctt ttaaagagta cactttatat tcaaaatctt
    12061 ttaagatttc acctatttct tctagcttat gagcattatt agtagcaatc actacttcat
    12121 taccataatc catacccaat atctcatctg ctatttcacc taaagcttct ctttgaagcc
    12181 ttattaattc tttattccct ttttcaccta actctaacaa taaatttaaa tctgctctcg
    12241 aaaaaggttt ctcttctcct gtcccctgta cttctacaaa ctcacactta tcagtcatta
    12301 ttatattcat atctacctgt gctttagaat cttcttcata acataaatct agcatgtgta
    12361 ttccatttac aataccaaca ctaattgcag atacaaaatt tgttattggc atctctttta
    12421 ttgtctttgc tttatataat ttatatattg cttctgcaac tgctacaaat gcccctgtta
    12481 tagatgcagt tctagttcca ccatctgcct gaatcacatc acaatctacc caaatagttc
    12541 tttctcctaa tttattcaag tctacaacag accttattgc tcttcctatt aacctctgta
    12601 tttcttgtgt tcttccatca atttttcccc tagatgcatc tcttatcttt ctttgttgtg
    12661 ttgctcttgg tagcatagaa tattcagcat ttatccatcc attaccacta tttcttaaaa
    12721 aaggaggaac tttttcttcc actgatgcag tgcatataac ctttgtctct cccatttcta
    12781 taagaacaga accttctgca tactttgtat aatctctagt tatctttaca ggtcttatct
    12841 gttcgttact tctatcataa cttctggtca ttttttatat cctttctcat attttttatt
    12901 ttttataaat ttatttttat aatttttaat tttgtcaaat attaattatt gtcatttttc
    12961 gtaatcttat tagcttactg aattattcta tcattaatta tagtagtttt ctactaaatt
    13021 aagcgacttt ttaactctct aatacagctt ttacttcatc tttcgatgat aactttaata
    13081 attctaaaaa tctctcttca tctataactt taattcctaa atcatttgct ttagtaagtt
    13141 tagaaccagc ttcttcacct gccaaaacaa atgttgtcga tttactaaca ctagatgtag
    13201 cttttccacc tcttttttct atcatatctt ttgcatcatt tctttttaat gtaggaagtg
    13261 taccagtcaa tacgattttc atcccttcaa atattttttc tactttttca cctgtattat
    13321 ccaatgattt tgtatttact cctacatttt ttaacttgtt tataacgttc atatttttgt
    13381 cttccttaaa aaactcgaca acgctgtttg ccattatact tccaaattcc tctaattctt
    13441 ctagttgctc actagttgca gaaattactt catctaaatc cttaaagtta tatgcaagta
    13501 tcttagctgc tttaactcct ataaatttaa tacctaatcc atttataagt ctccataaat
    13561 cattttcttt tgatttttca attgaattta ttaaattgtc agcagatttt tcgcccattc
    13621 tctctaggtc gactacatct tcctttttta tataatataa atcagataca tcttgaataa
    13681 ttttttcatt taatagcagt gtaattatag attctcccaa tccatctata ttcattgcat
    13741 cccttgacac aaagtgtata attccccttc ttatttgggc tgggcaagac atattgatac
    13801 attttacagc agcctctcct tctaatctta ttgttggctc tgaacaaact ggacactttt
    13861 ctggcatttt aaaatctatt tcatctccag tacgttcttc ttttataact tctaaaactt
    13921 gaggtattat gtcccctgct ttttgaacta atactgtatc atttattttt atatcttttt
    13981 ctcttatata atcttcattg tgaagtgtag ctctacttac agtagtacct gcaagtctga
    14041 ctggttcaag aattgctgaa ggtgttattg taccagttct cccaacttct actattatat
    14101 ctactatttt agttttcttc ttttctgctg ggaatttata tgcaattgcc catctaggac
    14161 ttttggctgt atatcccatt tcgtctcttt gttttatatt gtttactttt attaccatac
    14221 catctatttc gtagtctaaa ttacccctgt tatctgtcca gtattcaata tgctcaataa
    14281 cttcatctat acttttacaa actttgtaac tctgacttac tgaaaatcct agtttttcta
    14341 agtactctag tgattcactg tgactttcta aatcgtattc ttctatattt tccatattaa
    14401 atacaaatat atctaatggt ctctttgctg ttagttttgg gtctaattgt ctcaatgaac
    14461 cagctgcaag attccttgga ttagcaaata atggctgttc ttgttcttct tgttgttcat
    14521 ttacttttct aaaattatct tttgatatat atacttctcc tctaactatt agttcttttt
    14581 tatcatttat cttaagaggt atgcttttta ctgtcattaa gttttgagaa atatcttctc
    14641 ctacaactcc atctcctcta gttgctcctt tttcaaactc tccattgtta taagttagac
    14701 ctacagaaag accatctatt ttaaattcaa caacatattc aacattttct cccacatatt
    14761 cttgaactct cttatcaaaa tctctcaaat cttgctctga aaaagcattt gataaactca
    14821 acataggagt tttatgtaca atttgattga acttatctaa tggcttacct cctaccctat
    14881 ttgttggaga atcaattctc tttagttctg gatgttcttc ttctaaagaa attaactcct
    14941 tcattaactt atcgtattca taatctgata tttctgggct atcttgatta taataccttt
    15001 cattatggta gtttattgtc tctataagtt tattaatttc atcttttacg ctcacttttt
    15061 gcacctcttc atatttttaa aattaaatta ttttgaaatt tccctataat taattatctc
    15121 ctatttatat attttttagt atatcacttt tttggcttta aatttattac aaaaaaaact
    15181 cttattagta tctttaatac gactaataag agtttttatt tttatcaaaa ttttttattt
    15241 ttaatagcct cttgatatgt ctacaacatt atctacaggt ttattctcca ttatatagtg
    15301 tttcaaatta ttataaacca tattaaatgt tctctctctg tttttatctg acacccaaga
    15361 attatgagga gttactatga cattttcgca ctcccacaac ttattgtcct tgtttaaagg
    15421 ttcactttca aatacgtcta atgctacccc tctaaatttc tctacacatt cttctaaatc
    15481 tttctgatta actgtatttc ctctacctac atttataaat acagaaccat ctttcataag
    15541 tttaaacata tctttgttta ctattccatc tgtacttttt gtagaaggca ttgctacaac
    15601 tactatatca cattcttcaa atacaatatt catatcctca gtagcaaaac acttatcaaa
    15661 gtatttttta ctacttccat cagtattgac accccatatt tctacaccaa aagcttttaa
    15721 tctttttgct gcttctgaag atatagtacc tgttcctaaa aatccaactc tttgtccata
    15781 caattctgat acagaaaaat ccatcttcca ttctttattt tgttgttgtt taaaaaattt
    15841 tgcactattt ttatatattt ctaaaatata caatacaata ctttcaccaa taggtacact
    15901 atatccgcct ctattattag ctatcttaat atttctcttc aatacctctt cttttggaag
    15961 ttggtctata cctacactag ttgtctgtat atattgtaaa tttttcatct tagaaatatc
    16021 taatctctca aatggattgt atgtaactag tacatctgct gtatttacat cctcattatt
    16081 ttcaatacta tcttcagagt aatgtatgac atcatagcct aattctctta ttttatcaaa
    16141 tttttctttt ccataattta ttgtaaataa aactttcatc tcaaatctcc tccatctacc
    16201 cacatttaaa attttatatt ctaaaatata atatgatact caatgtacat tagttaaaat
    16261 tactataaga tatcttaaat ttacttatgt tcaattgttt ttcctaaaga catagcatta
    16321 cgttcttgaa ctaaattttc tattaaatta ttcaatttcc aatcaataga tgtctctgtt
    16381 accatagctg taaaaggtct atttattctc acttttttta gcccttctaa aaatgcattt
    16441 acttgtgtgc ttggagtgtt attaaatatt acagacttac tcttaatccc attttcacat
    16501 tcatttgata tatttccatc taaaatgtct cttattatat tctctccatt tttatcactt
    16561 aagatgatat ggtctcttat tccagcaaag ttacttacgt ttttaattaa tgataattct
    16621 tttttattaa agtttacaag cataatacac ttcctgttgt gtattttatc taggttatta
    16681 ttatttattt tttcaaatga cattttatcc atcctttcat ttatttatat actttaattt
    16741 accccataca tttaaaataa tcaattttta tagttaattt ttatattaat acccaaatta
    16801 tatttaaata gttaggctat ccaaaactaa tatataaaat tagaataatt agattaatat
    16861 ctacaaatca taacctaaag gagagatacc atgaatacat tactaaacaa taaatttagc
    16921 attaaaaact tttcaaatgc ttgggttata gtacttggtt gtatgttaat acaagctgtt
    16981 ccatttagta ttgtagcaag tatacagcct caatttataa gttatgttgt agaaggagaa
    17041 ggctttagtt tagcttcttt ttctctaata ttcactattg gtactattgc ctcagcaatt
    17101 gcatcacctt ttataggtgt attattttct aaagtcaata ttaagatact atatttatta
    17161 ggttgtataa tttctagtat tgcttttgct actttttcta tatgtactga actatggcaa
    17221 ttttacactg tatcatgtat tgttcaaatt ggcgctacaa ttatatctgc tataggaata
    17281 cctgttttaa taagtgcttg gtttgatgaa gaaagcaaag gaaaagcttt gggctttgct
    17341 tttgctggtg gttctattgg tagtatattt ctacaaccac ttgcagttcg cttattagca
    17401 actcaaggat acagtcgttc ttatctaata tttggaatta tagcattaat agttggtata
    17461 cctgtaatat tatttatact caggatgcct aaagatatta atgaaaaggt aaaatctaaa
    17521 ggtaaaaaag agaaagcatc tgatacaatt tctccttcag tatggggtta tacattaaaa
    17581 gaagctaaaa aaattaaata tttttggatt atgactattg gatttatatt tgttggtata
    17641 tatgtttcat cattatctgt acaatatcca acttatctta gaaattatct aaatcctatt
    17701 ctaataggaa cagtaggctc cacatttgct ttatttgctc tcattggtaa tttatttgga
    17761 ggattattgt ttgataaatt cggtcaaaca aagccactta ttatatcttt tgtattggtt
    17821 cttatagcaa ctttatcatt actattggta gataaatctt ctacttttgc atttgtattt
    17881 gccattttga aaggtctttc tgtatttgct tatatgattg gtcctgctta tttaactggc
    17941 tctttctttg gtcaaaagga gtttggtagc atatatggaa tcgtacaatt aatttttgca
    18001 gtgggatttg cattaggttc aacattattt ggattgttag ttgataattt tggatacaga
    18061 ttttcttggt ctattataat tttatcagtt ataattgcct atactttact tattataact
    18121 tctataggaa tgaaaaaatt gaataaagaa agattagata aactaatgca ataattgatt
    18181 tttagatata taaatattat aataaaaaag atggagcttt taatcataaa taaaactcca
    18241 tcttttttat attacaatat agctaatttt ctcttatttt taactttact atctatatcc
    18301 tcatccttaa ctatagtaaa tgccttagtt ggacaaacat ttacacatgc aggcccttct
    18361 ggcgattcta tacaaaaatc acacttattt gctgtcattc tttctctaaa gcaaagcttt
    18421 ccttcatcta catcttttaa tgtaactatg tctattgcac ctataggaca tgctaacata
    18481 caagtcttac atcctataca attttcttca ttgattttta ttgtgttacc aaccctaact
    18541 ataccaccat ttggacatgc ctttgcacaa ggagcatctt cacaatgtct acactgtatt
    18601 ggagcactta cttcttttgt ctttatgaca gaaagttttg gattaaaatt tatttcatct
    18661 ggattttggt aaaaaatatc ttcatctgaa tgtgcaacta cacatcctat catacaagtt
    18721 ctacatccta tgcatttatc agggtctgct ataacaaaat agttcatcac ttagccactt
    18781 cctttcctat acaatatgta gtgtgtaata gatgatgaga tttttctcct aatggttcta
    18841 ccagatattc atcatacagt tttttaatat caggattctc atgagatttt ctaattgcta
    18901 aatgttcatc atgcacgtat gttgcttttg ttctattttc ataagccaat tccttatatt
    18961 cttctagtaa aatttttggt tgacctccac cacttacaca cccaacagga caagtcataa
    19021 cttcaataaa atctacatct aattttcctt tttccaagtc ttcaagcact ggtattacat
    19081 tttttaatcc acttactact ccaactctta agtctaagtc tcctatttta atagttgact
    19141 ttctaaaacc attttctcct cttacttgtt ttacttctac atcaggaata ctttctccag
    19201 ttataagctc atatcctgtt cttatagcag cttccataac cccaccagta acaccaaaga
    19261 tagttccagc tccactataa ctacctaaag gactatcaaa tttttcttct cttaaatttt
    19321 taaaatcaat tcccatgtct tttattaaat aagctagttc tctagtagtt aatactacat
    19381 ctacatctct atatccacta gaatccattt cttctctatc acattcatat tttttacatg
    19441 tacatggcat tatagaaaca ctaaatatct ttgatgcatc tacattattt atctctgcac
    19501 cataagtttt aaatattgca ccacccatct gctgaggtga tttacaagaa gataaatgat
    19561 tagttaattt aggataattt ttttccatga attttaccca agctggacaa caagatgtaa
    19621 acattggaag agttccacct tctgtcactc ttttaattaa ttcactacct tcttccatta
    19681 tagttaaatc tgctgcaaaa ttagtatcgt agactctatc aaaatttaaa gctctaagtg
    19741 ctgctgccat tttcccagca cctaagtatc ctaaataata tccgaaatct tctcctaaag
    19801 caactctaac tgctggtgca cattggacca tagtgaatac atttgggtca cttaaagctt
    19861 tcttcacctt atctatatca catacattgt atgctgcaaa tataggttca tttatagact
    19921 ctggtatctc tctttcttgt tttttctctt gtaaaaactc aaatccttca tctataactg
    19981 aggcataaga tttacaagtt tgaacacatt gaccgcacat aacacatcta tctaaattta
    20041 tctcttgtgg ctttccctct tcaccttcaa tagcatttac tggacagacc tttgaacatt
    20101 ctctacatcc agtacataaa tccttatcta tatttattat cataaacgtc acctacccct
    20161 gtatccttta tttatttgtt aacaataaac taagtgcagc ttgtctattc ttagcttttt
    20221 tgtcatctat tggagtaaca agttttaatg cttcctgagg acatgcactt atacaagcta
    20281 ttttttcatt gtctttacat aagtcacatt tataagctat cttcttattt tcatctaaaa
    20341 ttacttgttc tacttcttta ccatcttcat attgtggaag taagtctata gctccaaaag
    20401 gacatgctag cagacaagtt ttacatccta tacacttttc ttcatttaca gacataatat
    20461 tgttttcttt aactatagct ttttgtggac atgagtttaa acaaggagca tcttcacaat
    20521 gtctacactg tattggcata cagaaattgt caccttttac taagtaaagt ctaggtataa
    20581 caggtatatc aactgttcca actgtataac ccacattatt atgctgatta tgcattgtaa
    20641 aacaagctac ttcacaagct ctacaacctg tacatttgtt tgggtctgct attacaaaag
    20701 agcctatttt atttttcata ttatcccacc tttcaatttg tatatttcca accctctagt
    20761 tattttcatc atatgtctac ttactttcat catatatatt tatatatttc actattttct
    20821 cagtacttat atttcgttta cattttttgc acaaattgta tgtgttatta tacaccttat
    20881 cagtatcgtc aattaatact ccacagattt cacactttga acatttttgt tcttcttttt
    20941 tagtacacat atacttattc tcactttttg ataactctct gtggatttgc ataaatattc
    21001 atccgtcttc ctcttacaaa tccaataagc gttatattta attttttaga aagctctatt
    21061 gataaattag tcggtgctga ttttgatact acaataggaa tttgagtcat agctgctttt
    21121 aatatcattt ccaatgatat tcttccactt acagccacaa ttttatcttt taatgttatg
    21181 tcatttaaaa cacaaaatcc aatagcctta tccattgcat tatgccttgc tacatcttca
    21241 caagtgacta tttttttatc aaaattaaat acagatacgc tatgtacccc accagtattt
    21301 ttaaataatt ctgatgaatt taaatttcta tccattatac tgtaaatcgt atcataattt
    21361 atttttatat tgctctttac aggagcacat gttatatagt ctagtgaatt taaaaatata
    21421 atttgctcat cttgattttt ttgtttctct tgaatagtta cttttgcaaa acgttcactc
    21481 tcatttatct caaattttaa taagcttttt ttgttttcta tgtatccttt tgttttcaag
    21541 aatcctacta taagttcctc taaattatat ggggtacata aaaatgtatt tatatattta
    21601 tcatttaaaa taaaactaag tggatattct gcaattattt cttcattatc tattgatact
    21661 tcatgttcat ttattttgct tacttctaca ttaaaagaat ttaaatattt gtagtccact
    21721 agactataac tattttcaaa aactttatcc ttactatttt cttgtaattt agtttttgag
    21781 tatatattgt tcataaaata ttagcctttc tgccttttat aaatcttaaa tctaataaat
    21841 cattagatat ttgtagctgt cattttgtct ttcaaacttt gatactcttc tcttacacat
    21901 ttttctgcta attcttggtc ttcaagtttt tcaagtttaa cagcacaata tttatactct
    21961 ggtgttttag atatagggtc taaatttgct attgtaagtt cattgcaagc tccaacccac
    22021 cactgataag tcatatatgt tgcaccttcc ttaactctat cagtaactgt tgctcttgtt
    22081 attacacttc ctcttcttga actaattcta actaattcat catcttcaat tcctaatttt
    22141 tcagcatcat tagaatttat ttgaacacgt cctggttcat cttctaatga actaagtgtt
    22201 ctacaattac cagtcattgt tcttactgaa tagtgtccaa cttctcttac agtacataaa
    22261 ctaaatgggt attcatcatc ttcaacttcc atagggggtc tccattcagc agcgaacaaa
    22321 ttacctttac cattaggagt agaaaacttt tgaccttcat acagatacat agttccttta
    22381 tcttccatac tttcactctt acaaggccat tgaacgcatc cttgagtttc tatcttttca
    22441 taagtagcac ctaagaaact tggacataat tgtctaagtt cgtcccatat ttccttagta
    22501 tttttataat tcataggata tcccattgct gtagaaattt cgcttataat ttgccagtct
    22561 ggttttacat ctccttgtgg ctctatagct tttctcatca attggaatcc tctatctgca
    22621 catgtatata ctccctcatg ttctccccaa gaagtagctg gaagtataac atcagcgtga
    22681 agtgctgtct tattcataaa tatatcttga acaattacaa attccaattc atctaatgct
    22741 tctctaactt ctgatgcatc tgggtcactt tgcactgggt cttctccaaa tatataataa
    22801 gctttaagct ttttctcttt tagtactaag tctggaactt gagttaaact ataaccatta
    22861 ttaggagata attttacccc ccaagctttt tcaaactttt ctcttatctt atcatcagtt
    22921 acattttgat agccaggata tacattagga agagctccca tatcacaagc tccttgaaca
    22981 ttattttgtc cacgtacagg tcctattcca acgctttctc taccaaaatt acctgtaagt
    23041 agagcaattg atgcaagtcc cttaactaca tcaacagctt gaccaaattg acagacaccc
    23101 attccataaa gtatcatagc ttttttacct tttgcatatt ctctcatagc ttttcttata
    23161 agctcttctg ggattcctgt aatcttttca gcatatttag ctgtatatgg tttaacaatt
    23221 tctttatact catcaaatcc ttgtgtatga ttttgaacaa attctttatt gtaaagacct
    23281 tcttctatta atacatttcc aaaagcattt actaaaacca tgtttgttcc accttttata
    23341 ggtagatgta tatcagcaat tcttgcagat tcagttactc tagggtcagt aacaattaat
    23401 ttagcaccat tttttttagc ttttactatt ctatttgcca ctattggatg tgaatctgct
    23461 ccattatatc caaatataaa taaaacatct gcattttcta tctctggtat agaattagac
    23521 attgcaccac tacctaatga gtaagccaga ccggctacag atggtgcatg tcagacacga
    23581 gcacaatggt caacattatt agttcctatt gtagctctca taaatttttg cattatatag
    23641 tttgcttcat taccaggtcc tcttgcagaa cctgttccca taatagaatc aggtccatat
    23701 ttttctttaa tttcatttag tcttgaagct gtatagttta tagcctcatc ccattcaacc
    23761 tcttctagta caccttgttt tcttatcatt ggctttttta gcctaggtgt tagaatttta
    23821 gggtcattta gaaaatccca tccataacga ccttttaaac acaaactttc ttgatttgtt
    23881 ctaccattag ccggttcagc tcttaatatc ttattatctt ttactactag atataattgg
    23941 catccagctc cacagtatgg acaaactgtt aaaatttttt tctccataca attcccccct
    24001 tagatttatc ccctgataag taaaatttat cttaaagata atgagtgttt ttataatatg
    24061 caaaatcata aatattttgc aatactatct attaaattca acaattttct cacaattcct
    24121 cttaaatttc aaaaaaaata tgtcataata atctttatat ttcaaccatt taattaaaat
    24181 attctagttc ttatgacata ttttttaata tactcaatat atattcttta aacttattta
    24241 gttttcactt aaaacaatat ttctgccaat tctatttttc attctatagt gagtaattac
    24301 agtaaatatt ccacttccaa tcagaatcaa atagtacata aatccaccat ataaaaatac
    24361 tgcatatccc atcaaaggtt gagggaatac tgatttgaat atagtgaaaa aggctaattc
    24421 attagctcct acattcccag gtgttggtat tggagatatt gccatatata aaaatgcttg
    24481 taatgtcagt atataaatat agctataacc ttgtaaatta aatgctttat atatccaaaa
    24541 tgaaacacta aagtaagcac ttaattgaat aaaagttact attatagttg aaattaatac
    24601 ctttttattc tttgcaaaaa aatttattgc tttactataa tcatctataa aactttctat
    24661 ggattcaatc ttattatcta aaaattttaa aaacttaatt tttgataaat gctttatagt
    24721 aattctcata aaatatttta tttttttagg atttaaaata actaatatta tcataattaa
    24781 gacaaatgaa ttaatggcta tccctagaaa cactagaggc ataatcattt tcatttgttt
    24841 ttctaacata gcaaaattca ttaaaacaag tattgtacaa taaaaagtaa ctactatttg
    24901 gaagataata gacttattgg taacaactgc acttgcctta ctaagtggca ttttacattt
    24961 tttaagtaca tatatctgta ctggttggct tcctgaagca aaaggagtta ttaaattata
    25021 ataaaatccc ataatggcta gcttaaaacc aataaatcta atattaactt tgtgagtgct
    25081 ttctataatt atcttcatta ctactccttc aagcattata tgacacatta ttgccaaagc
    25141 acctatgaat aaaaacttct tatctaccat aaccataaca tttgatagca ttttaatatc
    25201 caatgtttta aacactaggt aaattgttat acctattaac aaaaataaaa atgtatactg
    25261 tagtaaactc tttctaatat tctttagtct atccattaga aataaccatc ctttgctcaa
    25321 gatttatact aaa
//
LOCUS       DAELJO010000036        25194 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELJO010000036 DAELJO010000000
VERSION     DAELJO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.036"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(150..338)
                     /locus_tag="KPB88_001305"
     CDS             complement(150..338)
                     /locus_tag="KPB88_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053601.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            complement(364..1503)
                     /locus_tag="KPB88_001306"
     CDS             complement(364..1503)
                     /locus_tag="KPB88_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0053602.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKDIYKVNDKYLKMLDID"
     gene            complement(2041..2880)
                     /locus_tag="KPB88_001307"
     CDS             complement(2041..2880)
                     /locus_tag="KPB88_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0053603.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(2904..3662)
                     /locus_tag="KPB88_001308"
     CDS             complement(2904..3662)
                     /locus_tag="KPB88_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0053604.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(3709..4182)
                     /locus_tag="KPB88_001309"
     CDS             complement(3709..4182)
                     /locus_tag="KPB88_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0053605.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLDRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(4211..4627)
                     /locus_tag="KPB88_001310"
     CDS             complement(4211..4627)
                     /locus_tag="KPB88_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0053606.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNYGDSI
                     DEFSRRLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(4674..5726)
                     /locus_tag="KPB88_001311"
     CDS             complement(4674..5726)
                     /locus_tag="KPB88_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0053607.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVKSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDKISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDEDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            6122..6802
                     /locus_tag="KPB88_001312"
     CDS             6122..6802
                     /locus_tag="KPB88_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0053608.1"
                     /translation="MEKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(6935..7255)
                     /gene="sugE"
                     /locus_tag="KPB88_001313"
     CDS             complement(6935..7255)
                     /gene="sugE"
                     /locus_tag="KPB88_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG0053609.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            7637..8464
                     /locus_tag="KPB88_001314"
     CDS             7637..8464
                     /locus_tag="KPB88_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0053610.1"
                     /translation="MNFYKIAVCQMITTENKIENINHTVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            9271..9945
                     /locus_tag="KPB88_001315"
     CDS             9271..9945
                     /locus_tag="KPB88_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053611.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNGLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            9933..11093
                     /locus_tag="KPB88_001316"
     CDS             9933..11093
                     /locus_tag="KPB88_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0053612.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASILTTFSYILLNNIYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIETLDENISIIAKQEGKSFTLKSQINNMNLL
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKSALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHNGSISIENNSTNSAKITAKFSTRN"
     gene            11268..11936
                     /locus_tag="KPB88_001317"
     CDS             11268..11936
                     /locus_tag="KPB88_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053613.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHSEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11939..14407
                     /locus_tag="KPB88_001318"
     CDS             11939..14407
                     /locus_tag="KPB88_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053614.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDNRVHQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTASFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     VGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLI
                     FSFISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDNM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASTVIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(14650..16074)
                     /locus_tag="KPB88_001319"
     CDS             complement(14650..16074)
                     /locus_tag="KPB88_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053615.1"
                     /translation="MKNQSLTAQFRRTFIFIIIASIVSTVITYVFALYLYIYSLGKDI
                     YPPNHYELQIPRIEKYINEKNIALLSQSNEEGLKRTIRGNDMLYQVVDDNGNILYGTN
                     PKKPFKTKEELFNNFINTTVRNGGYIYTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIIALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIVLGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALIQKMQYTSDLEKSNIQLNLVPINLPEFLMQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(16093..16785)
                     /locus_tag="KPB88_001320"
     CDS             complement(16093..16785)
                     /locus_tag="KPB88_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053616.1"
                     /translation="MSKILIIDDEIDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMDGFEVCQAIRDDVLCPVIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLWLYMKERAIKIDGKDIDLTKIE
                     YGIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(16837..17616)
                     /locus_tag="KPB88_001321"
     CDS             complement(16837..17616)
                     /locus_tag="KPB88_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0053617.1"
                     /translation="MIKLLSSEWLRAKRTAVQGLTLCMPIIFSLCVVAYLTIKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVHEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMNIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGAKVWAFVPWTWPVKLGMLPSAYFIKEAGTISTEA
                     FYSKVMQTASIEFIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(17613..18323)
                     /locus_tag="KPB88_001322"
     CDS             complement(17613..18323)
                     /locus_tag="KPB88_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0053618.1"
                     /translation="MFFPVSFAAYAFIMQSTLMQNPLSQSTSWAWQNLLALIFNWWSF
                     VFLSIGFALFATLVASQEKKAGNYRALRTHNVYPMTLWINKVIAMAVYSFISTLVLIV
                     VTIITGLILKAGPIPFGQIIGASIVCWVVSLAILPIQLWLATWKGMFLSMGIGALGMI
                     FGVLASTEPFWVAVPWSWAVRMVCPIINVHPNGTVLEAGDPLLNTSVIPIGIVVSLVV
                     FLVLTALTAAWFNRRDDK"
     gene            complement(18676..18933)
                     /locus_tag="KPB88_001323"
                     /pseudo
     CDS             complement(18676..18933)
                     /locus_tag="KPB88_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19172..19429
                     /locus_tag="KPB88_001324"
     CDS             19172..19429
                     /locus_tag="KPB88_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053619.1"
                     /translation="MILNRTELFRGYSLNRFNKICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAEALINSKLR"
     gene            19572..20198
                     /locus_tag="KPB88_001325"
     CDS             19572..20198
                     /locus_tag="KPB88_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG0053620.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIDTDKIKTIMIN
                     EVVPTNPNDDFYSVDKDADYLKTTIPLFNSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20467..21246)
                     /locus_tag="KPB88_001326"
     CDS             complement(20467..21246)
                     /locus_tag="KPB88_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0053621.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21314..21775)
                     /locus_tag="KPB88_001327"
     CDS             complement(21314..21775)
                     /locus_tag="KPB88_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0053622.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21751..22428)
                     /locus_tag="KPB88_001328"
     CDS             complement(21751..22428)
                     /locus_tag="KPB88_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0053623.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22661..23047
                     /locus_tag="KPB88_001329"
     CDS             22661..23047
                     /locus_tag="KPB88_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0053624.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEGDIEELREILNKRTTKGDK"
     gene            23050..24849
                     /locus_tag="KPB88_001330"
     CDS             23050..24849
                     /locus_tag="KPB88_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG0053625.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGNNTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSQTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNTQTYLRQIGYGNYDVSGGISNFWLESSIKISPIEQVKLLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLSGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
BASE COUNT         9090 a         4086 c         3053 g         8965 t
ORIGIN      
        1 attatcatga ttaaagaaca taaatcatac acaaattggt cataaatctt acataaatcg
       61 tctacaaaaa atacattatt tatattaaat ctatataact gaattaagag catattgtac
      121 aatatgctct tagaaaagat atttcttgtt tatagtttat aatatttctc tatgattttt
      181 atttttatca tttcaaaaat aaaaattaag attacagtta ttatcatatt ttgtgtacta
      241 ttttctataa tattataatg aaacaatgaa aaaacaataa ttaatgaaat tacgtttata
      301 gctccagaaa tcaaaaatgc ctttttctta ttattcatat attccctctc cagattatct
      361 aaattagtct atatcaagca tttttaagta cttatcatta actttatata tatctttttc
      421 tttagacaaa atctctatct cttcaagatt attaaacact tccatcaact ggtcaactaa
      481 ttcaatttcc tttattccag cctttaagta tctactttta ttctttttta tatagttttc
      541 taatgtcata gaatagttat ctttattgca aatatatttt aatatatgtt cttctgttgc
      601 tgatagagtt atgtcatttt caaacaattc ctcttttaat atactttcta aagattttct
      661 atcatgcata ttacgcatct cattaataga ttgtattata gacttgagca taaactctat
      721 aaatacagtt aaattattcc ctttgttttc tacatcaagt atagccttat aatacttagt
      781 tctgttttta gatatcatat atgatattga aaactccttg aaagtatcat atcctttatc
      841 aatcaaatat agatatgata aggctcttga tgttcttcca ttaccatcac taaaaggatg
      901 aacatataca aagtaaaaat gaattatagc acttttaatt aaattactta ctttgctatt
      961 ttccataaaa tctattaatt tactcataaa gtcatatatc ttcaaaggtt caagccctgt
     1021 atgtataatc tcaccttttg aatcacatac atatactttt tcagttctgt actcatattc
     1081 ttctgaatcc aagcagtttt cgcccaatat atgatgcaaa tcatatataa atttgtgact
     1141 atacaattta tctaagttat ctaatccata ttctaaggct ctatgattat tgtaaatcat
     1201 atattcactt ttattacttg gtttcatctt tcctctaatt atagattttg caatctttct
     1261 agttgaaaaa gcaccttcta ttacactaga ataataagac tctagtgcca aatcattttt
     1321 atcttctatt gttttaaata tcttacttga tgttaattta tctatttcat ttattaaatt
     1381 taatatattt tcagtttcat gatagtatag ttctttatta tcaaaactat ttagagaaag
     1441 tacaatttta tttccagtaa agtatttgtt tttgtattct tctattgtca gtttaatcac
     1501 caccttagat tatctttatc tattaatatt aacaaaaagt attaattttt acactatttt
     1561 tgattatttg ttttataatt atcaaactca atttagcgtt aaagaataat aaattttaat
     1621 aaataatgca aaaagttaag aataagcatt taaaatatat gttacaggaa gtttacaact
     1681 atttataata cattagttat atattataat atctagacaa tgtcttaaca atatctcgat
     1741 tatatatttt aatatgtaac taatatatta taatatttta attaaattaa ataaatttat
     1801 atttttttga aattacttaa tattatatat gcaaatgaaa aagcacctca ttctgattaa
     1861 aaatctttct gagatacttt ttaaaatatt aaatactaca tatagcttat ataagtttaa
     1921 aatttaatct attataaaat caccctaatt gctaaacttt ttaatataaa ttttcccaca
     1981 tcttacaaat ataatcaata gatatagaat cctaatttac acaccaacat atatatttat
     2041 ttaaaatagt cccaaaatac ctgtaatagc tcccactact atcagtataa acattatttt
     2101 tattggagaa agaccttttt ttaaagcaaa gagtactaat aaagtaagta ccaaaggaat
     2161 taatcctgga aatatcttat caagtaaatc cgtttgaaga ctaaatttaa aatcaccact
     2221 agtataactt attggtgtag ataaattaat aaacctacct acaagtcccc ccatgactaa
     2281 agtacctaat atagaagccc cttctataac cttatttact attccacctt gaagtatact
     2341 ttcaacagct tttttcccat acttatatcc atttgtccac attccaaaag aaattgaagt
     2401 cattattata tattgagcta ttacaaataa tataggacct gcaattgatg ctcctccctc
     2461 agttaaacca atacaaacag ctaacactat aggagttata actccttgag ttaaagtatc
     2521 accaattcct gctagtggac ccatcaagcc tgttttgata gcattcatac catctccact
     2581 tatttcatct gctccattag ctttttcttc ttccatagct attgttattc catgtataac
     2641 acatccacat ataggttctg tgttaaaaaa tgctagatga gtctccactt cttgttttaa
     2701 ctcttcctta tctggatata attttttaag tactggcaac attgaatgtg aaaaagctgt
     2761 tgattgcaat ctttcataat tgtaattaga ttgtgcaaag aaaaaccatc ttaaccatga
     2821 ttttattaca tctttcttgc ttaacgtttt cttagatact tcatttttta tatctgacat
     2881 aatattttcc ccctcacatg tatttaagat gtagcactat cttttccgta cttaattgaa
     2941 gtaattgtaa gagcaagtat tactccaata acagatatta ctattatatc tactttaaaa
     3001 taaacagcca ttagtattcc taatatgaaa aagggcatag taaacttgtt tccaatcaat
     3061 tttaaattca tagcaatccc aagtgctggc aacatagccc caattacatt aaatacatgt
     3121 aacacattct cattcattac ttccaacatg ccatctatcg catttggacc aaaataaaca
     3181 actaataatg ctggtataaa actcattgca aataacagta tctgtggtgg tactatattt
     3241 ataaatgcta ctctatcaac attccctttt tgaacctctc tatcagccca atgtgcaaaa
     3301 aaagaattta ctgtcatctt tccaatccaa agtacagttg caacagttcc caatggtaca
     3361 gctattgcca ttgcagtact tacatctagc ttagatgcta tagctaaagc agtacctaca
     3421 tatcctgcaa aagctgggtc tcccatctga gctccccctg ctgatataaa acctagatat
     3481 atcatattta tacctgctcc tataattgct ccttgaacag ggtctcctaa tataattcct
     3541 gttacaaatc cagccaatag aggtctattc catgtaaaaa atccaacata agtcaaccac
     3601 ggactccaag aaagataata aaaaattgct attaatactg cttgaattag tgaaatgtgc
     3661 atattcttcc tccctatttt cttctaaaga tttacaagat agtcttcttt ataataatgt
     3721 tcctacatca attttctttg catctggtac tatttgtata aagcatttaa catcttttga
     3781 tattagctct ctaaatattt ctttttcttc atctgacaca gatatatttt tataaaaact
     3841 ttttctatca agcccagctc ccatacctcc aacattcaac tcctttaaat ctaccccact
     3901 atttactaaa cctaagacag ttttaggata tttgaccaat actattaaat tttcatctcc
     3961 attatgtgat tttaaaactt cagcgcctga ttctacatca tgtacttcaa ccttaacagt
     4021 tgatggagct gccatttgta aaacttgaac tataaatggg tcctttacta ctaaatcatc
     4081 cacgattact atcctttttg ccgatgtaat cttacaccaa gcagtaataa cttgcccatg
     4141 tattaatcta tcatcaattc ttgttaatgc tatattactc ataattattc ctccagattt
     4201 ttaatttatt ttacaacttt aaaacagttt taacatcttt gattcctgat atcccaactt
     4261 cctcacataa attcttcaag tcttttaatt tcatagtatc tctgtttaaa aataactcta
     4321 aaagcatagg taaatttaca cctgtcaaac attctaactc acatacctta gcaaatttat
     4381 ttattataat agcagatgta ttaaatggag tcccgccata aaaatcaagc aatactaaaa
     4441 ccccttcatc cttatatttc tcaattccat cttctaacct tctactaaac tcatctatgc
     4501 tgtcaccata atttaatcct aaagtttcta cattttcctg ttttcctact attaattcag
     4561 cactttctaa cattgcttta gccatatttc catgtgtggc tataattatt cctgccatat
     4621 gaatcatacc tttcttaacc ttttttatat ggattattat ttagtactct aatctaatta
     4681 tctatattaa tcattatctt accaaagaaa atatcccttt tgtatacttt ttcaaaataa
     4741 gctggtactt cttctaaatt tattctatgt gttactaatt tttcaacatt catatcaccc
     4801 ttttgcatat gatacaatcc agcactccat tcttttcctg ggaatggaaa ggagtttcca
     4861 aaccaagttc cctttacagt taattcactt cttactatct tttcaaattg ctctctagtc
     4921 aaagctacat ccccataagg tacacctgca tacaatactg tccctccctt tttagctagc
     4981 aacagtactt gaccacatgt cattggagta ccagccgatt caataacaat atctgcacca
     5041 tcttcatcag ttaacctttt tatttcttct acaatattct tttcttttgc atttatacaa
     5101 atatctgccc ctaactcttt tgccaagtct aacttctcat caaatacatc tacagctatt
     5161 atttttgtag aaccaaatat ctttgcccat tgtattgaaa ataatcctat tgggcctgta
     5221 ccaagtacta caacagtatc tccaacttta gcttctgacc taaaaagacc atgtcctgct
     5281 atacatactg gttctagcgc agcggcagtc tcataactta tcttatctgg tatttttatt
     5341 aaatttctct ctttcacctt tgtatactct gcaaaacaac ctccaagttc tttatttcct
     5401 attattgcta cattattaca ccttgaatac aatccttttt tacattcatc acattcaaaa
     5461 catggcatgg ctggacaaac agcaactcta tctcctattt taaaactctt tacttcttta
     5521 ccaacctgtg ccacttctcc agaaaattca tgccctaaaa tttctccaac catatgtgga
     5581 cctgtctttg aatattttga tatatcagaa ccacatattc cagctacttt tactttaatt
     5641 attacatcat ctttttctaa tatttttggt acatccacat cctccactct tgtgtctctt
     5701 ataccataaa atctaactga cttcactatt aaccacccct actttttatt tgagaatata
     5761 tacttattgt ttatatgtta aatatctaaa gcaactttca tgccaatata tgtaagtatt
     5821 gtattcatgc attttataaa attatcatta aatgtgtgtc aaagtgtgtc aaaagtttct
     5881 tatattaaaa cttatgtgtg tcaatataaa cgacactaac attttatatt agttctctta
     5941 ttataagttt tataaaatgt actttttttg ttatttttat aaaattgttc ggaaatttcc
     6001 cttctatttg tgatataata ttaaatataa ggtatcaata taaattttca tgcgaatatc
     6061 agaacttttt tgacactaat gccttattca atataataat ttaacttttt ggaggttgtt
     6121 tatggaaaaa gtgtatcaag gaaaaactaa agatgttttt gaattagata atggaaatta
     6181 cttacttaaa tttaaagacg atgtaactgg taaagatgga gtatttgacc caggcgaaaa
     6241 tagtgtaggt ctaagtatag atggaattgg tagagctaat ttagagacat ctgttaagtt
     6301 ctttgaaatt ctaaacaatg ctggaattaa aactcattat gtaagtgcta atttagaaga
     6361 agcaactatg gaagttctac cagcaaaagt ttttggtaat ggattagaag ttatttgtcg
     6421 ttatagagct gttggtagtt tcttacgtcg ttatggttca tgtgttgaag aaggtgctaa
     6481 actagattgt tacgtagaag caactttaaa agatgatgac cgttgtgacc ctcttataac
     6541 atctgaaggt ttagaagctt taaacattat gactcaagct caatttgatt caatgaaaag
     6601 catgactcaa aaaatttcta acatagttcg tgatactata gctgaaaaag gtcttgaact
     6661 ttatgatatc aaatttgaat ttggattcta caatgatgaa gtaatcttaa tagatgaaat
     6721 agcatctgga aatatgcgtg tttataaaga tggtgtaata gttgacccta tggatttaac
     6781 tgctctttta ttagataaat agtatataca tataattttc aaatataata aacttataaa
     6841 taagataagc tatgaagtaa agacttttta aatgttgatt atttttaaac attttctcgc
     6901 ctttaatttc atagcttatt taaattaata tactctaatt agtagataat aactttaacc
     6961 ctacaattcc caccacaata aagcctatac atgcaattct tgatatacta ataggctcat
     7021 ttaagatagc catgcctaca atagctgtac cgacggtacc aattccagtc catattgcat
     7081 atgaagttcc aagtggaaga tgttttagtg ctaatgacaa aaaataaaaa cttacaatca
     7141 ttccaacaac agtaagtata cttggagtta atttagaaaa tccatgtgaa ttttttagtt
     7201 gaaaagccca aaacacttca aacaaacctg caacaaacaa aaataaccat ttcataaact
     7261 catccctctt ttcaaatata tctctcattt ttccaataaa ataacctgag aaatattata
     7321 aaatatctcc caggtttttg tccttccgtg ttcacaacaa aaactatgtt gtgggtttta
     7381 tctcggacca gaccaatcat taataattgc ggaaccctat aaaactctat taattattct
     7441 attttatcta taataatact atcaacatat ataaaaaaag tcaatatgtg ggttccatct
     7501 ataataaaaa taacttttat atacctttta atattcttat tcacttctct ccatacccat
     7561 ttaggtcaat ctaagaatat ataattttta tttaatatgt tataataaca taaaaatatt
     7621 gaaaggagta atttctatga atttctataa aattgcagta tgtcagatga ttactacaga
     7681 aaataaaatt gaaaatatca atcatactgt agatatggta acagaagctg ccatcaatgg
     7741 agctaaaatt gtagttttgc cagaaatgtt taactgccca tatgaaaata aatattttcc
     7801 taaatttgct gaagaatatc cgggtgaaac aacaacgata ttgagtaaat tagctgagaa
     7861 acatggaatt tatctagttt ctggtagtat tcctgagtta gaagatggaa aaatctataa
     7921 tacttgctat gtatttgata aaaatggagc tctaattgga aaacatcgta aaatgcactt
     7981 atttgacatt gaagttacag gaaaagttag ttttaaagaa tcagatactc ttacagctgg
     8041 aaatgatgtc actgtaatag ataccgaata tggaaaagtt ggtatagcta tttgttatga
     8101 tattcgtttc cctgagttat cacgtttaat ggctcttaaa ggtgctgaaa tagttatctt
     8161 acctgctgca tttaatatga ctactggtcc cgcccattgg gagttatcta taagaatgcg
     8221 tgctttagat aatcagatat tctatgttgg ggctgcacct gctagaaata tgaatgcttc
     8281 ctacatagct tttggcaatt caagaatttc agatccttgg ggaagaataa ttgctcaagc
     8341 tgatgaaaaa gaatgtatta tttatgctga cattgataga gatttaattc ctgacataag
     8401 acaacagtta ccacttctta aacacagaag aactgattta tatgaactta atactttgaa
     8461 ataaaacaga acataacccc ataaatcata aattaagtgt taaaaaaatt tatggggttc
     8521 tcattaattt gatttaaaat taatttcata gaaataatac taatattaat ttaattaaat
     8581 aatactaata attactaatg attatgctaa ttatcagtat ttactaattt aatcactatt
     8641 agttatttct tttattaaat atctttactt tttttactta ttatgtcttc tagctttaca
     8701 tttggattat ggtatctctt tctattttta gtcacatctt tccattgaat tgaattgctt
     8761 gcttagaaca ccaatgcaca catgccagac aatgttcaca attatgtaaa aatcttgctc
     8821 taccttcttc aatgttgata ttatctttcc taaaatttct tttaattaaa aaacaacatt
     8881 agttgatata tcaagtatat tttaagcaac tgaaaaaaac aataataatt gatatatcaa
     8941 ctaattaatg tagattaatt catttttata atttatatac tttttaatta atctaccttt
     9001 ttaattaaat ccttcctttc tatttatcca ttttcaactt aaattataag atttcatcag
     9061 ctgtattttt gcattccaat aagtattaaa tatcaaatat atattctatt aaaatttcta
     9121 ataatttcat ccttgattta tttaatatat aaaaatatac taactttaat gattaagttt
     9181 tgaaatataa ctaagaattt gagaatttat tgagattttt atactaaaat atacatacta
     9241 tacaaactta atttagaaag gagcattctt atgaacaata aaatactcgt cgtagatgat
     9301 gaggaaggta taataacact cttaaaagat tactttgaaa tgaatggtta tgaggtttac
     9361 actgctcaaa gtggaaaaga cgcattaaaa aaaatatcca aaaatccaga tattattcta
     9421 cttgatataa acatgcctga aatagatgga attgaagttt gtaatagaat tagagattat
     9481 gtatcatgcc ctattctatt tcttacagct agaattgaaa atactgacaa gataaatggt
     9541 tttcgtgctg gtgctgatga ttatattgta aaaccttttg atttagacga acttggagct
     9601 agagtatctg cccatttaag acgtgaacaa aggaagtcaa atcaaactaa aattaaattt
     9661 tataatggtt taacaataga ttacgaaaaa aggttaattt gtatagataa taatcagatt
     9721 tcactttcaa aaaaggaatt tgatatagta gagcttctct ctatgaatcc tggacaagta
     9781 tttgataaag aaaaaatcta tgatagagtc tggggataca atagcaatgg aaatagtgat
     9841 gttataatgg aacacattag aaagatacga atcaaactat caaaacatac tcttgaaaat
     9901 tatatagaaa cagtctgggg agttggttat aaatggataa aataaataat atgtcattaa
     9961 aaaaagcttt ctttgttctt actttatctt cactaattat agcatcaata cttactactt
    10021 tctcatacat tttgcttaat aatatatata attctataca agataagtat ttaaaaacac
    10081 ctataaatac aggtattata accatagttc aaaacaatgt gaaccaattt agtgattatg
    10141 ataaaacatt acttaatata atcaacatac ttcagcttgt acttccactt atatttttta
    10201 taggactttt attattagct gatataattt tttacaaggt aaaactaaaa acacccatag
    10261 aaatactcaa taaaggtgca atggaaatat ccaacaacaa tttagacttt tgtttagaat
    10321 ataataataa tgatgaatta ggtaatctat gtaatgcttt tgaaaaaatg aggtctgaac
    10381 taaataaaaa caacataaag atgtggacta tgattaatga tagaaaacaa cttaatgctg
    10441 ctttttctca tgatttaaga aatcccctaa cagttctaaa aggttactca aactatttaa
    10501 caaaatacat accaacaggc aagcttagtg atgagaagat tttatcaacc acacaactta
    10561 tgtctgagca tattaacaga attgaatact atgtagaaaa tatgagtaat gctcaacgat
    10621 tagaagattt agttgtttca aaatctatgt caaatataaa caagtttata gaaaccttag
    10681 atgaaaatat atctatcatt gctaaacaag aaggaaaatc ttttacactt aaaagtcaaa
    10741 taaataatat gaatctattg tttgatgaaa atatcattct tagagtcgtc gaaaatataa
    10801 tatcgaatgc ttttagatat gctagaaata atgtatccat acttatatat cttgaacaag
    10861 aactccttac ctttgtaatt gaagatgatg gtataggatt tagtaaagaa tctctaaagt
    10921 ctgctttaag acctttttat agagataaga ctttaaatga cagcaatgca cactttggta
    10981 tgggtcttta tatctcaaaa atattatgtg aaaaacataa tggctctatt tctattgaaa
    11041 ataattcaac taacagtgct aaaattactg ctaaattctc aacaagaaat taaagcaata
    11101 ttaaatcaat tttttaggct ttcacttctc ttttataaac tttattaact tagagtttac
    11161 agagtgtttt tttgaaataa atttttagta aacaaaatta aaaagttgag aaaaaattga
    11221 gttttaaaac ttacactctt cttatataga aaaggagagt gttttttatg attataaaag
    11281 caaaacaatt atcaaaaata tatggttcta ataacaataa agttatcgca cttaataatg
    11341 taaatttaga aataaactca ggtgaatttg tttctataat tggaccttct ggaagtggaa
    11401 aaagcacact tttacatatt ttaagtggat tagataatcc tacctctgga caagtgttac
    11461 ttgatgataa agatatatat aaacacagtg aaaaagaact atctgcactt agaagaaaat
    11521 cctttggatt cgtttttcaa cagtttaatc ttttacctgt tctgacagca tctgaaaata
    11581 tttcaatgcc tgtacttcta gataaaaaac aacctgataa agactattta aatgaaattt
    11641 catcattact tggaattgca gatagactca atcatcttcc ccatgaactt tcaggtggtc
    11701 agcaacaaag agttgcaatt gctagagcac ttattgcaaa accagatata atatttgctg
    11761 atgagcctac aggaaacttg gacagtaaaa gtggcagtga agttatgaat cttcttataa
    11821 aaacttcaaa acagtttgga aagactttag tggtaattac tcatgatgat agaattgcaa
    11881 aacttgctga tagaaaaatt tcaattattg atggtgttct tatggaggtg aaatagaaat
    11941 gaagaattac ctttcacttt caataaaaga attaaaaagc cagaaactta tgactacatt
    12001 catcattata gcaatagtac tatcaactat tatgacaact gtggttggac agtcaattgg
    12061 tatcctacaa aatttaagaa ttgaacaagc ccgttctttt aacggtgata gacatgtatc
    12121 ctttcaccaa cttactaaaa atcaagttga tgacataaag aaagataata gagttcatca
    12181 agctggtact tcaataactc ttggcagtag taaaataaag gattctggca tctctgtact
    12241 tgtaaaagag tatgataaaa caggactttc taactaccca aaattaatga aattaaaatc
    12301 tggacatttg cctaaagata aaaacgaaat agccttagat gaaaacacac taaagcttat
    12361 gggtataaaa ccaagactag gagtgactat acctatgaat ttagatattt cacttttaaa
    12421 tgataccata ccaccatata attacacagc tagctttaaa ttgagtggta ttttagagga
    12481 tgattataca ggatacgtaa gtggtatagt aaatggtatt gtaggtaaag gtacatcaga
    12541 aaatctatta cctaagagat atattttatc ctctttggat tttaaaataa aacagcaaga
    12601 aaagtttcaa gaaatagtaa atcaattagc taaaaaaata aatctttctc acaattcaat
    12661 tcaatataat tggatttact taaatgctct taaaatacag tttgaaaaag atgaaaatag
    12721 ttctaatagt gatggtatat ctatgattat attggtatct ttatttgttg ccttattggt
    12781 tttactggct tctggacttg taatctataa tattttaaaa atctctgtta caaagaaaat
    12841 taaagagtat ggatgtttaa gagcaatagg agctgaacca aaccagatat ataagatagt
    12901 tattttacaa atcttaattt tatgtactat tgccatacct gttggagctg taattggaat
    12961 tatatcttct aaaggcataa ctggaatggt cactaatatc ttaaatccag atatacttct
    13021 agcaaatgat aataaagaaa ttactgagct tatacataaa aacacaacag catatatgtt
    13081 cccattagta ttaagcacta gtatatcttt gattttttca tttatttcag cattaccatc
    13141 agcaatatat gcttcacatg tctctcccaa aattgctatg gctggcagta ctaccaaaat
    13201 caaaagaaaa attaaacgag aaaaaacaat aaaaaacttt gaaagacatc tagcatggct
    13261 aaacttaaaa agaaataaag gaagaactat aataaccata ctatcattat ttatgagtat
    13321 aacagtattc gttgcactga gtgagttctc taatgtatta gatgtatcta ggtcagtaag
    13381 taatttaaaa gaaggagatt tttccttaac aaatgaaata tctggttttg ataagtcaat
    13441 actagataaa atagataaca tgaaaaacgt caatcgcact tcttttataa agtactctga
    13501 atacaaacag ggcgaaatag atacagatat aaattttgaa aatagtggtg agatgttaaa
    13561 aatcattgga attgatgaac aaactttaaa agacttaatg ccttctatta cagattctac
    13621 attagaagac tttaaaaatg gttctatctg ttttataaaa aatccgattg ctatttctac
    13681 accaggtgta aaaactagac atacaaattt aaaacctaaa gatagtatca caattaacaa
    13741 aaaacaacta gatatatata gtacagtaga taaaatgttc tttttacaag gtaatggttg
    13801 gataaatggt gttgatgtta ttgtatatga ttctgtttat aacaccttga caaataaaaa
    13861 caagttcaac cagataaata tttatgctaa agataaatca aaattagaac aaataagatt
    13921 gtctattgag cagatttgtg aaaataatcc tggctctcat tggatatcat acattgaatc
    13981 agataagcaa cttaaagaga gttttaaaca aatagaattt ctagcatggg cagttatatt
    14041 atttgtggga ttgattggta ctttaaacat cattaacaca actcatacaa atataaatac
    14101 taggactaat gagattggag ttaaaagagc tattggtatg agtaatagta gcttatataa
    14161 gatgttttta tgggaaggtg tttattatgg aatttttgct gctatatttg gttcaattgc
    14221 tggatatgcc agtactgtta ttataaacat ggcaactata gaaaagcttg atttcactaa
    14281 tataccaatt acttcaattt tacaagctac tattatttct gtacttgcat gtattatagc
    14341 tactcttatt ccgttaagaa aagttaaaaa gatgaatata attgactgta tagatatcaa
    14401 tttataacta ttttacagat aataatataa attaaattat caattactgt ctccattttc
    14461 tttcaaatct aactaataaa cctttttaac tataatataa gttttaaaaa aaggcctgtc
    14521 tcaaaataga gataagtctt ttttcattgt cggaataatg aaaatatatt taacttcaca
    14581 aaaaaaaaga gtgcctcacg aactaaaaat ttcgttttga gacactcccc tttagtttaa
    14641 aatatatagt taaatgctat gccaaaattt cacacatgct ccacctgatt ttgaattttc
    14701 tattttaaca gaacctccaa gctgttccac taattgtttg acaatgtaaa gcccaagacc
    14761 agaatgtcca ccttttgtct gcctagcttc atctcctcga taaaacttat caagagcctt
    14821 ttttaaatct ttagagctaa acccactacc agaatcacaa atctcataag aaatacaatt
    14881 tttttcatcc tttacagtaa tactaatatt accacctgat ggagtgtatt gcagactgtt
    14941 ggatatgata ttatcaaaaa ttcgtgtcaa cctatccacg tcgatttgaa ttggggattg
    15001 tatattaccc tgcattttca atataagttc aacttccttt tgatgagcct gtaattcata
    15061 gtcttgtacc ttttgcatca aaaattcagg taaatttata ggaacaagat ttaattgtat
    15121 atttgatttt tctaagtcag acgtatattg catcttttgt ataagagcag cacttttttc
    15181 tgtattttct cttatcactg taagatacct atgaagtttt tcattatcat ctgtattatt
    15241 accaataagt gcatctgtat atcccaaaac aatagaaaga ggagatttta aatcatgtgc
    15301 tagagcttcc accatttcaa cacgttcctg ctccatcttc cactgtgcag ataaagaacc
    15361 ttttaattcg tctttcattt cagaaaaggc actacaaagt ttaccgagtt cattatctga
    15421 ataatagtcg atttcaaaat caaggtcctt ttcttttatc ttcttggacg catcaatcaa
    15481 tagatgtaat ggttgattta tattctttac aaagcgctta gaaagccatc tagtaaaacc
    15541 cacaatatat agaaatggag aaaacagagc tattataata actgcaaata caaatctccc
    15601 tctactattt gcaaaggtga ttttcacttg gtagaataga ataacagctc cctcaatctt
    15661 tccattatcc cctttgattg gaacagtata gatataacca ccgttacgaa cagttgtatt
    15721 gataaaatta ttaaataatt cttcttttgt tttaaatggt tttttggggt tagtaccata
    15781 tagaatatta ccattatcat ccacaacttg atatagcata tcattcccac gaatcgttct
    15841 ttttaaacct tcttcattcg attgtgataa caaagctatg tttttttcat tgatgtattt
    15901 ttcaattcta ggaatttgta gctcataatg gtttggtgga taaatatcct tacctaagga
    15961 ataaatatac aaataaagag caaatacata agtaatcact gtggaaacta tactggctat
    16021 tataataaaa ataaaagtac gtctaaattg ggcagtaagg gattgatttt tcatttaata
    16081 acctctctct ctttacattt tattccactt atacccaatc ccccaaactg tggaaatgta
    16141 ttctttttct ggtgtcacag cagaaaactt agctcgtatt tttttgactc gttcaacaac
    16201 agttgaggaa tcaccttcag aatcacaccc ccatactttt tcatagattt gttctcttga
    16261 gaaaacttgt cctgcatgga gtgctagtaa ttcaacaata ccatactcta tttttgttaa
    16321 atcaatatcc ttaccatcaa ttttaattgc acgttccttc atatataacc ataactttcc
    16381 aaaatacaaa gtagacctct tgttctcctc attgatatac tgtgaacgct tttccctacg
    16441 cagattagct tcaatccttg ccataagttc acgcagtcca aatggctttg ttacataatc
    16501 atcccctcca aaggtcaacc ctttgatttt gtcggtttct gattgctttg cacttaaaaa
    16561 tataactggg cataaaacat catctctaat tgcctgacac acctcaaatc catccatttt
    16621 aggcatcata atatccagta taataaggtc aggctcctgt tttgataact ctatacctgc
    16681 ctgaccatca taagctatca acacttcatg tccttttgcc ttcaattcgt cctcaagtag
    16741 cattactagg tctatttcat catcaataat taatatttta ctcaatataa caccaccttt
    16801 ctcttaaggg aaggtcagta ttaaaacaat attcgtctac tcataacttt tccttccctc
    16861 ccacatttta aaccaaataa cgcctcctat caagaatata atcaaaccta ttgctactac
    16921 tataaactct atactagctg tttgcatcac tttagagtaa aatgcctcag ttgaaatagt
    16981 tccagcttct ttaataaaat aagcacttgg aagcattcct agttttactg gccatgtcca
    17041 tggaacaaat gcccatactt ttgcaccaac gctagttaat ccaagtattg ctgccatcaa
    17101 aattccacca atactaatac caatagatgc acccataccc catgcaaaac tcacccaaag
    17161 atgaatagct aatagtggaa gcgtcccaat caccacaagt cctgctgctg acaggaataa
    17221 ccatattgtt gctccagatg gcactgcaat attcattcct atgcagagta tcaacgtagc
    17281 aataaatgtg catatagtta ggcaaaacag aagaaaaaga aatttgccca gatataatct
    17341 aacacgagaa atacctacac ataaaaaacc attaaaattt ccagcaagct cttcctcatg
    17401 cactataaat cctgctaaaa tacctactcc aataggaatg atgaatacag tccatactgt
    17461 aaaaaatcct tcaaaagcaa attcttgtgt acttccagat ttaattgtga gataagcaac
    17521 cacacataat gaaaaaataa taggcataca aagagtaagc ccttggacag ccgtcctctt
    17581 tgctcgaagc cattctgatg ataatagttt tatcatttgt catccctcct attgaaccaa
    17641 gctgcagtaa gtgctgttaa tactagaaat accaccaaag aaacaacaat cccaatagga
    17701 atcactgaag tattcaaaag tgggtctcca gcttctaata cagtaccgtt aggatgtacg
    17761 ttgatgattg ggcataccat tctaactgcc caactccaag gtactgcaac ccaaaatggt
    17821 tctgtcgaag caaggacacc aaatatcatg cctaaagctc caattcccat acttaggaac
    17881 atacctttcc atgtagctag ccatagctga attggaagta tagcaagaga aacaacccag
    17941 catacaatac ttgcccctat aatctgtcca aaaggaattg gcccagcttt caatattaaa
    18001 ccagttataa ttgttacgac tattaaaact agcgttgaaa taaaactgta gactgccata
    18061 gctataactt tattaatcca aagtgtcata ggatacacat tgtgtgtacg taaagcacga
    18121 taatttccag cttttttttc ctgggaagct actaaagtag caaacaatgc aaatccaata
    18181 gacagaaaca caaatgacca ccagttaaaa attaatgcta aaagattttg ccacgcccaa
    18241 gaagttgatt gagataatgg attttgcatg agtgttgatt gcattataaa tgcatatgct
    18301 gcaaaagaca ctgggaaaaa aatatactat ttcgttcaat ttgtagagaa acattgttaa
    18361 ctgccatttg ttctccatat ctttttgata agttttttgt ttcaagaatt aaatctttca
    18421 tattaatttc tcccttcttg taatacctta aatctattac taagagaata ctaacatgaa
    18481 aaaataacaa ttctataaca cctactaatt ttaataaaaa ttaaaataaa aaatagagtt
    18541 accaatttta ttttggcaac tctatttaag tttatgtttt acagttatat tagatttaag
    18601 cttagtttca ttatcacact aacttcaaat ttactctata atccttaaat caagcactaa
    18661 tttattaatc aaacactaat ctattaattt atatttcttt agacatctaa tatagtttgc
    18721 tactaaataa taattattta tatcatattt atctaatcct ttaaaagcag ttatttcatc
    18781 attaagctca tcactacctt ctacaaaaac acaacctgtt tcaaattcca tacctacaat
    18841 aatcttacag ccactataag tttcctcttg agtatcgtac aatttttttg catttccttc
    18901 atttggaatt gtaaatacaa taggttttaa ttcttaaata tgtcttacta attctctaca
    18961 ggatatcccc gtagttccta cgattcttac ataagttatc tattttaaat tgttattagt
    19021 tagattctaa aaattattta ttttatcact aatataatac tcttaaaaac tttacaaatc
    19081 aatttaaatt tgctaccttt tatttataac aacttatagt acatttctcc tatgcttttc
    19141 aatatattac ttttaaaagg aggttttcct tatgatttta aatagaactg agcttttccg
    19201 aggatattct ttaaatagat ttaacaagat atgcaatata ttatcagtaa ataaaattaa
    19261 atataagtat aaagtgaaaa ataatgctta ctctaaaaaa ccttttttta atgaaataac
    19321 acttggcaat ttaggtcaaa aggaagattt ctcttatgag tacttaattt atgttcataa
    19381 agacgattat gaacaggcag aggctctaat aaattctaag ttgcgataga ttatttataa
    19441 aacatgacat tctgttgtca tgttttatat gttattatac tttcaaatat gctactatat
    19501 tttcaaaatt gtgtgcttta aaactatctt tttatagtaa tttaaagctt aaaactaagg
    19561 agactcgtta tatgaaaata aatataaaat caaagttaag tgaatttatt aaacagaaca
    19621 atctttttga tgacaacaga ataatatcct accttcctaa tataacaata gacacagaca
    19681 aaataaaaac tataatgatt aatgaggttg tcccaactaa tcccaatgac gatttttata
    19741 gtgtagataa agatgctgat tatttaaaaa ctactattcc tttatttaac agtgcaggaa
    19801 tagaagtatc taacataaat gatatcctag atatgggtat ctatataact aatgcagtaa
    19861 aacttcctaa atcagaatat acaataactc gtgatacaat taaattacat atgcctattt
    19921 tagaagaaga aattaaatta tttaaaaacc tagaggtagt aatgttaatg ggagatgttg
    19981 caaagaaatc ttttaatatg attactaaaa agcatataaa aaagaatgtc atcccttcca
    20041 tttcaacata taaacttcgt aataatgaac tttattatga taatttaaga gtatttccat
    20101 catatattat gactggtgga aacatactga ttgaaaaatc aaaatttgaa atggcatcag
    20161 aagatataaa aaaaatgttc gagattataa acggttgaga ttacaaaata ataaaaacta
    20221 atttagacaa ataataagtg cctcaaaaaa ttctatctat gaggcacttt tattttatat
    20281 tagtgtaact atatactatt aacataacta tatactatta acataatttg ttatttatat
    20341 tgttcttttt aatctctatt ccatttagtc taggtaacat tactctttac aagattactt
    20401 tatatttctc ttatatctct aacattttta tttaataaaa caattattaa tacaagtcaa
    20461 ttatatctat ttatctaaaa atatatcttt cacagtaata tctggatggt gatacttctt
    20521 cctattcgtt aaaaaagatt tttttacatt aatagcctct tttggacacc agtgaaaaca
    20581 agctaggcat ctttcacact cattacccca ctttggcttt ttatcaacta tacttatatt
    20641 ctctacagga caaacccttt tacatgtccc acatccatta catttatttt ctgcaaaaaa
    20701 tttagtagtt gcaatttgca tattcttacc tactaaacga agtacaacac tatctatatg
    20761 tgactttaaa ttgttttttc cttctatatt tccaatctcc aagttattga tacattttgc
    20821 aatctcagct accctattag tctgattttt caatctttct tcttcaacat ctggaggtgt
    20881 tatcaaagca tttccaggca tattgattaa aaaaccagca gataattttt tccccctttt
    20941 tttaagtaac ttatctatag taaataaact atgtccagct accccattac aagtggcaat
    21001 ggcatatata tgagtattaa tattaaaatt taatttctct acaaaagact ttactatatc
    21061 aggaacatca gcaaaataaa caggaaaaac aattaccaat atatcttcat ttatgtctat
    21121 attattttct tttctatgta tagctaaagg tataacttct ttacttccat ttaacgtttc
    21181 ttttaaaatc tttgaaacta ctagcgaatt tcctgttcca gaaaaataat aaatcttact
    21241 attcattctt atccctcttt ctatatttct acatcatcaa tagttaaaaa gtctcttaaa
    21301 ataggattta tatttacttt ccaattctat ccaaaatatc agtaaacaaa ggcaattctt
    21361 catcagagat agatttaaat gtctctgttg catatatact tgcaatttca tttaatggtt
    21421 cttctaattc tctacctaaa tctgttagga atatttgaaa cttacgtcta tctttaacat
    21481 ctactttacg gacaattaat cccttatttt tcataacatc taagattctt gtcgtattag
    21541 atttatcctt acatgatttt tcacataaat ctatctgaga aattccatct tgctcccata
    21601 gaaaccttaa taacgcataa tggtcagttg ttatatcata tccattttgt ttaaacatct
    21661 tttttaaatc atcttttact tttaacgaag tacttactaa cttagttgta gcttcatata
    21721 gtttctcaaa atctttcatt ttacctccat ttaaataatc ccttgctcct cccatctatc
    21781 aattattccc ttatattgct caatatcttt aaaagccatt ggctttaaaa cagcatttat
    21841 agcaataaaa ttatagattt gagaaagcac aggtggattt ttcttttcct ctatgcaata
    21901 aataaatttt ttggatacct tttgtatctt tgattctact ttttcagtta taggattttg
    21961 attaaatcca gtttttatag ataaactacc acaaacataa cctcccgaat ctaagaccat
    22021 ggattttata aaagaaattg cttttgaacc acgagcattt tcatatgtag taactgctat
    22081 acatggctta cgatacaata atctttcaag catcatatgt gctctatctg taaacacttt
    22141 gaaaagtcca gttacactac ttccataagt tgggctacca aatataacac catcactctt
    22201 agtaattata tcatgaatat actctacttt atcattttga ttaatacact tccccatttt
    22261 ataacaattt aaacaaccaa tacaatatga tggattgacc tcacttaaat caaaataatt
    22321 aatttttaca tctggtatta atctttctat atctttatac aaataagtca aaacttttga
    22381 tgtagctcca ttttttctag ggcttccatt tataactgta attatcattt ctatctctcc
    22441 tttttagttg ttatatcaac aataagtata tcaactatat ttttagctgt caatataaac
    22501 cctttattta aagctatact tacacagctt aaataaaagt tttatttaac tacatgtgct
    22561 tatttaatca actataaatg aattattttt aataattgac aaactaaatt aatgataata
    22621 tactttatgt attacaaatg taagtagaag gaggtatagc atgaaaaagt taccacatat
    22681 ttcagaagca gaatatcaag ttatgaaaat tatttggaaa tatgctccta tcagcactac
    22741 tgaagtaatt gaaaagttgg tagaaacaag tacatggagt cctaaaacaa ttcaaacaat
    22801 gttattgaga ttagttaaaa aaggagcttt aacatatgaa aaaaatagca gagtattcgt
    22861 ttatactcca cttgttaaag aagaagaata cgtagctaca gaaagtagtt cttttttaaa
    22921 tcgtttttat aatggtactc ttaattctat ggtactaaac tttttagaaa atgataaact
    22981 atctgaaggt gatattgagg aactgagaga aatcttaaat aaaaggacga ctaaggggga
    23041 taaataaata tgtatgcccc attctttata cgatttttac tcagtacatt aaccttatca
    23101 atattaattg ttgtaatttt acttgccaaa aaagtattta aaaaacatat ttctgcaaag
    23161 taccaataca atattggatt tatattgctt ataatgttaa taattccatt tgtccctctt
    23221 aaatatatta atcttggaaa tgtatttaac tatttatata cttttaatca acttggaaac
    23281 aatactaata ggacttctat aataaaaaat agtccaaatt ctataattaa taactcaaat
    23341 gcattaaacg atttttcagt atccctaaac caagttaatt ttgatagttt aaatatttta
    23401 ttactaatat catggataat tggaatttta atcatgatag ctattactat tcattgcaac
    23461 attaaaataa aaaatatgaa aaaatcaata caaattttaa gagatgagga gatagtattt
    23521 atatttgaaa agtgtaaaaa agatattggt ataaataagg attttatact atgcaaatca
    23581 cctattataa atacacctat tacatttgga tactttaaac cacacattat attaccagat
    23641 aagtctatat ctaaattatc tttaaaagat ataaaatata tcttacttca tgaattacaa
    23701 cattttaaaa ataaagatat actgataaat tatataatgt gctttcttca aatattatat
    23761 tggtttaatc ctcttgtatg gtatgcattt aaagaaatgc gtatagaccg tgaaattgca
    23821 tgtgatattt cagttttaaa aaagcttgat aaagatagtc atatagaata tggacaaact
    23881 ctaataaact ttgtggataa tatttcaaga ccatcaaatc ttacacttac atcagatatt
    23941 ggtgggtcaa aaaaacaaat aaaaaagcga ctattgaaga taatggattt tcataatgaa
    24001 tcaaatacgt taaaattcaa aagcatttta atttttacat tgatagcttt aattgtattt
    24061 ggaaatattc ccaaactatc tattatggca gatatcaatg aaaaatatag acctacaaat
    24121 gaacaaactg tgtacgagga cttaagtcag tattttaaag gttttgatgg aagttttgtt
    24181 atgtatgatt taaaatccaa gcaatatcat atttacaata aagaaaaaag tcaaacaaga
    24241 atttctccaa attctactta taagatttat agtgccttat tgggattgga aaatgggata
    24301 ataacaagaa ataattcaca tatcccttgg gatggaaagc atcattctaa tagttcttgg
    24361 aatgaagacc aagacttatt ttctgctatg gaaaattccg taaactggta ctttcagact
    24421 ctagataaaa aaacagggct aaaaaacaca caaacttatc ttagacaaat tggatatggc
    24481 aattatgatg tttcaggtgg tatctcaaac ttttggctag aatcttcaat aaaaatatca
    24541 cctattgaac aggtaaaatt acttgaaagt ttttatacaa ataagtttaa atttgaagat
    24601 aaaaatatag aaactgtaaa agattcattg ttaatatcaa aaaaggatgg ggtatcactt
    24661 tctggtaaaa caggcactgc caatataaat ggaaaagatg taaatggttg gtttataggt
    24721 tatgtggaaa aaaatgaaaa tacttatttc tttgctacaa atattcaaag caaagattat
    24781 tctaatggta gttcagcatc taagataaca ttatccatat taaatgacaa aaaaatctat
    24841 aatccttaat atatttactg tacattctaa aaaattcaca taattttcat attaattatc
    24901 gattaaattc atatacaagg cttattatat aaacatagaa gatagtaaaa ctagcttaaa
    24961 aacataatat aataccctat acaaatagtg gcaactctcc ctcacatcct caattgttgc
    25021 cactatttaa ttttttgtat gaagttaaat atattttcat catttcaaca attggggtgc
    25081 agtacactct ctattttgaa acaacctctt taattgtttt agagtttatg aattatttct
    25141 catactttaa aaatgctgta ctgatgtaga tattgaagca tttgaaaata ctaa
//
LOCUS       DAELJO010000037        24781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELJO010000037 DAELJO010000000
VERSION     DAELJO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.037"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            197..442
                     /locus_tag="KPB88_001331"
     CDS             197..442
                     /locus_tag="KPB88_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0053626.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            477..1202
                     /locus_tag="KPB88_001332"
     CDS             477..1202
                     /locus_tag="KPB88_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0053627.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1224..2537
                     /locus_tag="KPB88_001333"
     CDS             1224..2537
                     /locus_tag="KPB88_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0053628.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            2743..3282
                     /locus_tag="KPB88_001334"
     CDS             2743..3282
                     /locus_tag="KPB88_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0053629.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3371..5356
                     /locus_tag="KPB88_001335"
     CDS             3371..5356
                     /locus_tag="KPB88_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG0053630.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5580..6548
                     /gene="spoIIIAA"
                     /locus_tag="KPB88_001336"
     CDS             5580..6548
                     /gene="spoIIIAA"
                     /locus_tag="KPB88_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG0053631.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6538..7059
                     /locus_tag="KPB88_001337"
     CDS             6538..7059
                     /locus_tag="KPB88_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG0053632.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7076..7273
                     /gene="spoIIIAC"
                     /locus_tag="KPB88_001338"
     CDS             7076..7273
                     /gene="spoIIIAC"
                     /locus_tag="KPB88_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG0053633.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7289..7675
                     /gene="spoIIIAD"
                     /locus_tag="KPB88_001339"
     CDS             7289..7675
                     /gene="spoIIIAD"
                     /locus_tag="KPB88_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG0053634.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7760..8965
                     /gene="spoIIIAE"
                     /locus_tag="KPB88_001340"
     CDS             7760..8965
                     /gene="spoIIIAE"
                     /locus_tag="KPB88_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG0053635.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9025..9642
                     /locus_tag="KPB88_001341"
     CDS             9025..9642
                     /locus_tag="KPB88_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG0053636.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9654..10208
                     /locus_tag="KPB88_001342"
     CDS             9654..10208
                     /locus_tag="KPB88_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG0053637.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10235..10924
                     /locus_tag="KPB88_001343"
     CDS             10235..10924
                     /locus_tag="KPB88_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG0053638.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11034..11396
                     /locus_tag="KPB88_001344"
     CDS             11034..11396
                     /locus_tag="KPB88_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0053639.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11481..11987
                     /gene="nusB"
                     /locus_tag="KPB88_001345"
     CDS             11481..11987
                     /gene="nusB"
                     /locus_tag="KPB88_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG0053640.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11971..13041
                     /locus_tag="KPB88_001346"
     CDS             11971..13041
                     /locus_tag="KPB88_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG0053641.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLDSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13038..14243
                     /locus_tag="KPB88_001347"
     CDS             13038..14243
                     /locus_tag="KPB88_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG0053642.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14245..14460
                     /gene="xseB"
                     /locus_tag="KPB88_001348"
     CDS             14245..14460
                     /gene="xseB"
                     /locus_tag="KPB88_001348"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG0053643.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14463..15350
                     /locus_tag="KPB88_001349"
     CDS             14463..15350
                     /locus_tag="KPB88_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG0053644.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15380..15817
                     /locus_tag="KPB88_001350"
     CDS             15380..15817
                     /locus_tag="KPB88_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG0053645.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15851..17716
                     /locus_tag="KPB88_001351"
     CDS             15851..17716
                     /locus_tag="KPB88_001351"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG0053646.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDEGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17849..18661
                     /locus_tag="KPB88_001352"
     CDS             17849..18661
                     /locus_tag="KPB88_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG0053647.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18681..20360
                     /gene="recN"
                     /locus_tag="KPB88_001353"
     CDS             18681..20360
                     /gene="recN"
                     /locus_tag="KPB88_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG0053648.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKKVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20575..21441
                     /locus_tag="KPB88_001354"
     CDS             20575..21441
                     /locus_tag="KPB88_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053649.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKYGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21534..22025)
                     /locus_tag="KPB88_001355"
     CDS             complement(21534..22025)
                     /locus_tag="KPB88_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0053650.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22086..22526)
                     /locus_tag="KPB88_001356"
     CDS             complement(22086..22526)
                     /locus_tag="KPB88_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0053651.1"
                     /translation="MKLNVRIADINNWRDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22687..23745
                     /locus_tag="KPB88_001357"
     CDS             22687..23745
                     /locus_tag="KPB88_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0053652.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23887..24711
                     /gene="spo0A"
                     /locus_tag="KPB88_001358"
     CDS             23887..24711
                     /gene="spo0A"
                     /locus_tag="KPB88_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG0053653.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
BASE COUNT        10075 a         2495 c         4531 g         7680 t
ORIGIN      
        1 gtaataggtt aagttagaat aacccattac ctatttttat ttgtaagaaa agtgtacata
       61 gaattagaag taaaaaatac aactaataaa actattgaaa atactagata aataataaat
      121 aaaaataatt atagattttg gcatacatat tgctaattat agagataaga cgacaaaata
      181 attgggaggt ttaatgatga aatacaaaac aacagggact tgtgctacag aaattgaatt
      241 tgaagtaaaa gaaaataaag ttacaaatgt aaactttata ggtggatgtg atggaaattt
      301 aaaagggcta aaagtattag ttgaaggcat gaatatagaa gatgtaatac aaaagttaaa
      361 aggaatagag tgtaaaacaa agccaacatc ttgcccggac caactgtctc tagcattgga
      421 aaattatatg aatataaagt aatacataaa acaatcgatt agttaggagt ttaattatga
      481 aaccaataat agggatttta ggaaatttaa taataatgga aaatggtatg tttccaggtc
      541 ttgaaaggtc atatgtaaat aatgattata taaatgcagt gctaaaaggt ggtggtagtc
      601 cagtaataat tcctgtaaat acagataaag aagttataaa aaaacagatt gaaatggttg
      661 atggagtctt aatatctggg ggatgggata tcaatcctca actgtatgga gaagaaacaa
      721 gagaagaaac gacatttata tatccagaag tggatgaatt tgatttaatt gcaataagta
      781 tagcattaga acttaaaaag cctatattag gtgtatgtag gggactacaa attttaaatg
      841 taagtttagg aggaacttta tatcaagata ataatcttat agaaggaagt tatataaaac
      901 atacacaatc ttctaaaaga catgtagcta ctcataaagt tgatgttaaa gaaggaagta
      961 ttttagaggg aattttagga aaacaattac ttacaaatag ttatcatcat caaagtgtca
     1021 atcagcttgg aaaaggatta aaagccatag catactcaaa agatggtata atagaagcta
     1081 ttgagaaaga ggatgaaaat tttgtagtag gagtacagtg gcatccagaa atgatggtag
     1141 attactgtga taaaatggaa aaactattta aatattttat aaatatttgt tcaaatttat
     1201 aatatacctt ggggggattg aacatgaacg aaaagaacaa aatgggatta ataagtatta
     1261 tacttttagg tattaatgca gtagtaggag ctggagtttt tctattacca ggagatgcaa
     1321 tgaaatcatt tggtgtagca agtatatttg tatatatatt tgatatgttg ttagttttat
     1381 ctatggcatt ctgttttgct gaggttgcag gtaaattcaa taaaaatggc gctgcctatg
     1441 tatatacaaa agaagctttt ggagattttt gtggttttga agtaggtctt atgaaatggg
     1501 taataggatg tatttcttgg ggagctttaa tagtaggttt tcctacatca ttatcagcag
     1561 tatgggctcc agcaggtgaa ccacatatac aaaaaattat aattgtagct atgatagttg
     1621 ggcttacaat tataaactta ttaggtgtat ctctatcaaa aattgtacaa aatgttataa
     1681 ctgtaggtaa actaattcca ttaatattat ttataggaat aggtatattt tttataaaag
     1741 gagttaattt tacttcttca acaatggtac ctccaggagc tggagcaaca gagtttggag
     1801 cagcagcact tttaatgttt tattcattta caggatttga atctatagca gttgcagcag
     1861 aagatatgga gaatccacaa aaaaatatac caatagcaat tataagtgta atagtaattg
     1921 catcaataat atatatatta aatcaagttg tatgtgttgg tatactagga gattcgttat
     1981 ctagtacatc tacaccagtg gcagatgctg caagaatatg ctttggaaat atgggagctg
     2041 gattggttac ctttggaacc ttggtttctg ttggtggaat ttgtatgtgt ggtgcttttg
     2101 taaatccaag aagttgtgta gctttagctg atgataaaat gttaccgaga atatttgcaa
     2161 gaaaagataa aaaaggtaca ccatatgtag cgataatagc tactatgcta atcactatac
     2221 caatagctct atctggaagt tttgctgaat tagcagcaat aagtgcagtt gctagattca
     2281 tacaatacat accaacatct ttatccgtat tagtgtttag aaagaaaaga ccagaattag
     2341 taggtacttt taaaactcct tttggagcag tgattcctct aattgctgta tgtgttggaa
     2401 catggttgtt attccaagca tctatgcatc aattaattat gggattagga gctttggcaa
     2461 ttggagtacc attatacttt ataatgaagt cttataatag aaaagtctat ggtgaaaatt
     2521 taaagaaaat agtttaatta tgttataaca gtttattgag ttaaaattag acattggcag
     2581 aaaattaaat acaaataagt atctataagg ctatattaaa atattattaa ttttttattt
     2641 taatatagcc ttaaattatt atcaataagt gaaaaatatt gtaacaatag aagaacaata
     2701 gtattatgtt tatagacaaa aaacttatta ggaggtaaat ttatgccaac aataacatta
     2761 aaaaatggtg tagacgtact tattagagaa ggagtaaggg aagatgctca aagcattata
     2821 gatttttata atgaagtggg tggagaaact cattttctgt catttggaaa ggatgaatat
     2881 aaaatatctt tggaggaaca agaaaatgct atagaatcag caaaagcatc tgataattct
     2941 gttaaactga ttgcatttat agatggtgaa atagttggga ttgcaacgat tgattctaat
     3001 caaaaagcaa aaggaaagca tgttggagta ttaggaattg ttgtaaaaga aaaatattgg
     3061 ggaattggat tgggtaaaag gcttatgtta gaccttatag aatggtgtaa atcaaatgga
     3121 ataaccaaga agataactct tgtcactaat gaagaaaatt ataatgctat aggattatac
     3181 aaaaaagttg gatttgaagt agaaagcata ctaaagaaag aatgttatta taatggtgtt
     3241 tatactgatt taataggaat gtcactttta ttgggaatat aattagtgta cattttatct
     3301 cgaaaaaagt atattaagta tggtataatt aaaaaagaat ataacttaat ataattttgg
     3361 aggacgaatt atgaaaaatc tgtgtaaaat atcagatgat tatttaaaca gcaagcttaa
     3421 gtatttaaag ttattatcaa aacaatatcc aagtatatca aaagcaagta cagaaataat
     3481 aaatttagag gctatattaa atcttccaaa gggaacagag cattttataa cagatgtaca
     3541 tggtgaatat gaaccatttg tacatgtatt aaaaaatggt tctggtgtaa taaagagaaa
     3601 aatagaagaa ttgttttcaa atacaataag agacagcgaa aaaaaaatgt tagcaactct
     3661 tgtatattat cctgaacaaa agttagacct aataattaag caagaggaaa atatagatga
     3721 tttttataga ataaatattt atagacttat agaactttgt aaatatgctt ctagcaaata
     3781 tactaggtct aaagttagaa aactgttacc agaaaatttt aaatacataa tagaagaact
     3841 tttgcatgag catgttaaaa gtgaacataa agaagagtat tataaaagta tagttgaaac
     3901 aatagttgac ataggaatag ctaaggagtt tataattgct atttctacag ttatacaaaa
     3961 attggttgta gatagacttc atgtaatagg agatatttat gatagaggtc caagaccaga
     4021 tataattgtc gataagctta ttgaacatca ttgtgtagat attcaatggg gaaatcatga
     4081 tatattgtgg atgggtgcag cctcaggtga aaaaacttgt atagcaaatg cactgaggat
     4141 atcagctaga tatgcgaatt tagacattgt agaagacata tatggaataa atctattgcc
     4201 tttagccact tttgctatag aaatgtataa agatgaccct tgtaaagaat ttattccaaa
     4261 ggttaatgac caaagtgtaa caacaacaga aaagtcttta atggcaaaaa tgcacaaagc
     4321 aataagtata attcaattta agcttgaagg tgaggttata agacgaagac ctgaatttga
     4381 aatggaacat agactcttgc ttaatatgat aaactatgat gaaggtacga ttaatttaaa
     4441 aggtaaaaca tataaattaa aagatactta tttacctaca atagacaaaa aagacccata
     4501 taaattaact atggaagaaa gaaatgttat tgataaatta gtatcttcat ttagaggtag
     4561 tgaaaaatta caaaaacatg tttcattttt gttttctaaa ggaagtatat atttaaaggc
     4621 taactcaaat ttactgattc atggatgtgt accgttaaat gaagatggaa gctttatgtc
     4681 tatgaatata atgggaaaag agtataaagg taaggctctt atggataaga tggagtctct
     4741 agcaagagaa ggatttttct ttaaagataa ggcagaagaa aaactgtatg gcatggatat
     4801 tatgtggtat ttatggactg ggaaatgttc atcattattt ggtaaagatg atatgactac
     4861 atttgaaaga tactttatag ctgaaaaaga gactcataaa gaaaataaga acccatattt
     4921 taaacttaga gaaaatgaga tggcttgtaa aagattattt gaagaatttg atttagaact
     4981 tgatgaatct catataataa atggtcatgt tcctgtggag agtaaaaatg gagagagccc
     5041 aataaaagct aatggtaaaa ttctcgttat agatggtggt ttttctaggg cttaccaaaa
     5101 aactactggt atagctggat acactttgat atacaattct agaactttgc aattagtatc
     5161 gcatgaacca tttaattctg ctgaagaagc cattgcaaat gaaagtgata tcttatcgac
     5221 aactgttgta gtagagcata aagcaaagag aaaaatggtg agagatactg atgaaggagt
     5281 aaagatacaa gaggaaatag aagatttaaa actactttta atggcttata agaaagggct
     5341 aataaaagaa atgtagatgt tatagaggca cttcatgatt gaagtgtctt tttgttaata
     5401 cttattaaat atattataaa gattaataat agtagtttat attatactta ctaagtgaat
     5461 ccaagataaa tgggttcact ttttttattt ataaacatta ttaaaaaaat atagatgttt
     5521 tggttatatt ccttgtcctt tataaataga attatataaa caaaggagga ggacaagaga
     5581 tgaataaact ttctgatgaa ataataaact ctctttctac aactataaga gaaaaggtag
     5641 aaaaagtctc aaacaataat ctgaacatag aagaaattag gttacgctca caaaaaccat
     5701 tgatactaaa tgcaaactct aaagattatt tttacaatca aaaaactatg acacttgatt
     5761 taaatcaaca aaactcatat gtagtaacaa gagaagatgt agagcaaact tttcaaataa
     5821 tatgtaaata ttcaatacat tcatttatgg atgacataaa aaaaggattc ataactttga
     5881 gaggaggtca tagagtaggg ttagttggaa aagcaatagt agaagatgga caagtcaaaa
     5941 atataaagca tatatcatct ttaaatataa gggtatcaag ggaaattatt ggatgctcgg
     6001 ataagatttt aagtcatatc ataaagggaa aaaatcagat taataatact ttgataatat
     6061 caccacctca atgtggaaaa acaactttaa taagagatat agtaagaaat ttaagtaatg
     6121 gtaatgaaga ttatggattt aaaggtttaa aagtggcttt agtagatgag cgtaatgaga
     6181 tagcaggagc ttatctaggt gttccacaaa tggatgtggg aataaggact gatataatag
     6241 aaacttgccc aaaagacttg ggtattacaa tgcttttaag gtctatgtca ccaaatgtta
     6301 ttgtgacaga tgaaattgga agtgaaaaag aaataaaggc attatacaca gcattaaatg
     6361 gaggtatagg attaataact actgtgcatg gagattcaat agaggatata caaaatagaa
     6421 aagaattaaa tagacttctg gataaagagt tatttaaaaa ggttataatt ctttctgcaa
     6481 aaagaggagc aggaacaatt gaaaaaattt atgatttaga agaaaagaga tggtattttg
     6541 caaattaaaa taattattat agctttcttg ataggaagta gttatttgat aggtgaacaa
     6601 atatacaaaa cttatactcg tagacataaa caattaaacg atttaataag agttttagaa
     6661 attttgagga tggatttatc ttttggactt tatacattag aagaaatctt taataggata
     6721 ggtggaaata aagagttttg tttttggaaa tttttctatc aaatatcaga aggacttcac
     6781 aatgaacaaa gtaaaacttt ggaaataata atatcagaaa atattgatgt cttaagtaaa
     6841 gaaacttatc taggcaataa agaaattgag gaactgaaaa atctaatact tactttaggg
     6901 aaaagcgata tagagtcaca acaaagaatg atagatttat ctatagaaaa tttgaaaaaa
     6961 caaacctatg agacaaagga agatataaac aaaaaaggcg tattgtataa aaaattagtg
     7021 acattcatag gtataggtat atgtattata ttgatttaat atggggaggt ttattatgga
     7081 aatatcattg atattaaagg ttgcaggtgt cggtattcta atatcagtat tgaatatgat
     7141 attggaaaaa acagatagaa aagattgggc aggtcttact acattagcag gtgtaataat
     7201 cgtattgggt atggtaataa cagaaataag tgacttgttt aatactgtaa gaacaatgtt
     7261 tcaactttac taaagaggag ggagtaattt ggaaataatg caattaatag gaattgcaat
     7321 catttcaact acactctgtt tagtaattaa aaaagataga ccagagatag caaatttcat
     7381 agctataata actggagtaa taatactatt gtcagttatg tttaagctaa attttatagt
     7441 agatagtata caagatttgg ctaacaaggc taacatcccg actatgtata tatcattaat
     7501 aataaaatta attgggatag catacttaat ggaatttgcg atacaacttt gtaaagattg
     7561 tggggaagga aacattgctt caaaactaga atttggaggt aaaataatag ttatgtcaat
     7621 gtctttccct atacttttgt ccattgtaga aatggtggtg aatataattc catagatgtg
     7681 tatcttggaa tgaatataag tatattagag agctttcgtt gtattgaggt ttatagagtg
     7741 aagagaggca tggttgctta tgaaaaaaag atttttacca atgattatag gttttttatt
     7801 tactttcttt tttgtaaata tctttgctat tatgatattt gctaatgaag taccaagtag
     7861 tgaagataaa gaatatggtg aaacaaaaaa tagcatagat aagtatatag atggtcaatt
     7921 agacaagcta gatataaatg agattcaaga ctatataaat aaagaaatag ttattaatga
     7981 tgttaattta aaatcatttg taaaagattt aattagtgga gaaaaaaata tcttagattt
     8041 attcaataaa gatggattga agatattgat gtttgatgaa tttaaagcaa gtttaaaggt
     8101 agtagcagta attttggtac tggctctgtt atcatctatt ttaaagagtc tggaaaactc
     8161 tttttcatca ggtgcagtaa gtcaaatagc aacatatata atatttatca caatggtatc
     8221 acttacactt attggtttta aagatgtatt acagatttgc tatgatgcaa tagaccatac
     8281 tgtaggtctt atgcaagtta taatgccaat attaataaca tttttacttt taattggatt
     8341 cccaataacg tcgactactt tgaatcctat cttcatagga ggagtaacat ttataaatgt
     8401 attttttaaa aattttctat ttgtatctat tactgttgca tttggaattt taataataaa
     8461 caatttatca aaaaatataa ggctaaagag atttttctcc tttgtaaaac aaataaatta
     8521 tgtttctatt ggagctatgt ttactgttta cttaggtctt gtatccatac aaggactgta
     8581 tgtaaccagt tttgataaat tcagtgtaaa gactgctaaa tttgctatag gtaattttat
     8641 tcctgttgta ggtggatttg tatctgactc tgtggatatt ttattatctt catctcagct
     8701 tataaaaaat atatttggag gaatagggct tatcttactt gttggaattt gcttacttcc
     8761 agtaataaaa attttatcag taatagtagt gtacaaatta gcagcaataa ttgtagagcc
     8821 tgttggagag gatggaatat ctaatttttt aaatgaagtg gcaaatttaa tgataataat
     8881 gctagcatca gtaattgcta taacagtaat gttctttgta actgtagcta tcttgacctc
     8941 gataagtgtc gtaagtcaag ggtaatttat atatcagtga aaaataatta catttaatct
     9001 aatgttttta gagaggagga tgctatgtta gagggcataa aggcatggat agttagtgtt
     9061 ttgataggag catttatagt aaatatagta gatatgattt taccaagttc taagataaaa
     9121 ccttatgtca acctagtttt aaactttatg tttgtattta ttgtaataac tccagtagta
     9181 ggattttttt caaaagatat gagtttagaa gatagaatat taaaatctat gggaaactac
     9241 aacaagcagt atgtagatag tacaaatgct ttggcaaaag agactggaaa taatagtcta
     9301 tccaagggat atgaggatgg gttaaaagaa gtacttaaat taaaacttga tgaatatggt
     9361 tatgatttag aagatataga gcttaatggg gcaaatataa ataatataaa aataaaagaa
     9421 aagaataata gtgcaaaaag taataacagt tccaatgaag aaaataaaaa taatagtact
     9481 aaaagagagg aagaaaacat aaactctaat gacaaagaaa attctaaaca agtcttcaaa
     9541 aaaggtacag agtatgggtt gaacctaaat gaagaaaaat taaaaaacga cttaataaag
     9601 gtgctggatg tttctataga agatatacaa attgataaat aggagttggg agaatgttga
     9661 aaaacttaaa tgacaaagat aagagaaagg tatattcact aataactata gcaggaatat
     9721 gtgtggtttc attagtatta ttatcttgtt ttcctagtag caaaagcgat aaacaagttg
     9781 gaaaaacaga gactaataaa aatgctgaaa agcaagtcac taaagaacaa gaaaaagatg
     9841 atttagagtc aaaattaaca gcaatactat ctaaaattga tggtgcagga gacgttgatg
     9901 taatggttac atttgaatca agtgaagaaa tacaacctgc ttttaattca aataatacaa
     9961 cagaaacaac agaggaaaaa gatgcacaag gtggagaaag aacagttacg acttctagtg
    10021 aaaataaaac aatgataact tcaaattcaa gtgaccctgt agttataaaa actacagagc
    10081 caaagataaa gggagtgata gtggtagcaa gtggagcaag tgacccaagt gttaaagaga
    10141 cactttatag tgcagtacaa acatcattac aagtagctgg tcatcaagta gaaatatatt
    10201 ctaaataaat acatataacc ggggggataa atgaatgaag tttaattata agggaagagg
    10261 atttgtaata ataactttaa ctgcaatgtt agtggtagta ggaacagtga attatcaact
    10321 aagtaagaaa tctttattag aaacatcaaa agagtttaag gcatatgaag aagcacagtt
    10381 gcaaaaaaat actgatgaga gtgatagtag taagacagag gattctagta atatagataa
    10441 acaagatggt aaggaaagtg ctgatattga tatagttgat agcaaagcta gtaaagtaaa
    10501 agaaaaggca actgagacaa gtaaggaaat aaaagctcaa ttatcatcag agaaaaatat
    10561 gaaaaaggca tcttatattt tagatatgaa aatgaataga gaaaaacaaa gaaatgagtt
    10621 agttcaagat ttaaatgaaa tgataaataa tccatctaca acagaagaat ctagaaaaga
    10681 agcttctaat atgaaattaa atatagttaa aattcaagaa aaagaacttc aaatagaaaa
    10741 ccttttgagt acaaaaggat atgaagaagc gttagtttat ataagcgata acaaagttaa
    10801 tgtagttgta aatgaagcta aattagaaaa gaaagatgca gcaaaaatat ttgatttagt
    10861 tgcagaacaa gccaatgtga agtatgaaaa tattaaactt acaaataata atagtaataa
    10921 gtaaagtata aacctcagaa ttaactgagg ttattttctt gcaaagtaga cttgtatttg
    10981 gtattattat acttataaaa atagttcctt aaaatcagga ggtaagtttt aatatggaag
    11041 ataataaatt tggacaagta aaaatatcta atgatgtaat agctacaata gctggtttag
    11101 cagcattaga agttgaaggc atagaaacta cagcaacatt aacagataag ttgttaaaaa
    11161 ataatggagt aaaaatacaa atcgaagaag aggatgttaa tttagatgta atggttacga
    11221 taaagtatgg aatgtcaata ccagatacag cttttaaagt tcaagaaaat gtaaaaaata
    11281 ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa tatacacatt caaggaatca
    11341 gctttaagaa ggataaagtt gataaagaag aagcaaaagc aactaagaaa aattaatatt
    11401 agccctctga aaagtcagag ggttttttgc tggtaattga ttttatatgg ataattaaac
    11461 ttaaaggaga attttgattg atgaaaaaag atagggcaca aaagagtaca acaagagaat
    11521 atataatgaa acttatatac caaataaata ttaataaaga agattttgaa actttagaag
    11581 ataaagtaga taatttttta aaagataatt cagaacatat tataaataga tataaagaac
    11641 ttgcacttca atactcaaaa aatacaaatt taaagttaga agatactgaa attgaagatg
    11701 ttatagacaa aaaatatata aatacagtat gcaaagcatt gaaagaaaat catgacaaaa
    11761 tagatgagtt aataaataag catgctaaaa actggacagt agatagaatg ccaaaggtag
    11821 atgtttctat acttagattg tctgtgtgtg aaatacttta tttagacact cctaataaag
    11881 tatcaataaa tgaagcagta gaacttgcaa aaatatattg tgatgataag tctcctaaat
    11941 ttataaatgg tatattggga agtgttgttg atgaaattgg gaaataatat aataattgga
    12001 attgatacta gctgctatac tacttctata gcagctatct ctttagataa gaaagttata
    12061 ttcaatgaaa aaataatgtt agaagtaagg gataattcaa aaggtttaag gcaaagtgaa
    12121 gctgtatttc aacatatcaa taatttaggg attcttagtg ataggataaa atcttttaaa
    12181 gataagttta atgtagaagg agtttgctca tcaaaaaaac cgagaccggt tgaaaactca
    12241 tatatgcctg tattcaatgt aggtcataat tttggaaaat tattgtctag tatatatggt
    12301 tgtaggtttt atgagactac tcatcaagag aatcatattg aagcaagttt attagatagt
    12361 aaattaaaaa ataataataa atttatttct gttcatatgt ctggtggtac tacagagata
    12421 cttttaacaa gcaaacaaga tagtcatcac aatgtatgtg atactaattt aggtaaaatt
    12481 gccaaaataa gtattaaaaa agatgataaa agtaagttat acaataattt tggatataat
    12541 atagatataa ttggaggaag taaagatatt agctttggtc aacttataga tagagtagga
    12601 attaagcttg gatataaatt tccttcagga aagtatttgg atgaaaatgc tttaaattgt
    12661 aatcttaaaa tagaaagtgg tctgaaaact tctgtaagag atgggtatat gaatttatca
    12721 ggattagaaa atcaggtaaa taagattata aatgataatg gagataatac taatcaaaaa
    12781 gaatatatat ctaaattagt attagattca gtagttagaa atatgtttaa atctctagtg
    12841 tatttatgtg aaacttataa tgtaaatgaa gtagtatttg ctggtggagt ttctgcaagt
    12901 aagtatattc ttagagaatt aagcatgaag ttaagaaaaa agcatataga agcttatttt
    12961 acagaaccac aatattcaac tgataatgct gtcgggtgtg ctataatagg attgaataac
    13021 tttttaggag aaagagtatg aaactaagag ctttagatat aagtgaagca aactcatata
    13081 ttaaaagaat tttaataaac gacccaatac tttctaatct taaagttaaa ggagaaattt
    13141 ctaattttaa ggttcatagt agtgggaatg tatatttatc acttaaagat gaaacatcaa
    13201 agttaaactg tgtaattttt aaaagtaatt ttaatcgcaa tttaaaatta gataatggtg
    13261 taaaaattat agctaatgga tatatatcag tctatgaaag agatggtgcg tatcaattat
    13321 acattaatga aattgaaata gagggaatag gaaatttaca catagagttt aatagattaa
    13381 aggaaaaact taataaagaa ggattatttg accctaaata caagatacct atacctaaaa
    13441 tgccaaactc aattggcgtt ataacatctc caacaggtgc tgttataaga gatataataa
    13501 atgtaataaa aagaagatat ccgaaagtta atataaagct ttatccagta acggttcaag
    13561 gagataaatc agctgaagaa atctgtgaag caattagatt tttcaatcat atgaagaatg
    13621 ttgatacact tatagttggt agaggtgggg gttcaataga agaactttgg tcttttaatg
    13681 atgaaatggt agcaagagag gtttttaatt ctcaaattcc gataatatca gcagttgggc
    13741 atgaaacgga ttttactatt tgtgactttg tgtctgatat gagagcccca acaccatcag
    13801 cagcagctga gattgctact ccatcattgg atgatataaa ctataaactt ggaaacataa
    13861 aaagtaggat gagtaagtct cttacaaatc aaatagagct agaccaatat aggttagaaa
    13921 cagtatttaa taagataaat aactatttag attcgtacac aataaaagat aaagttatac
    13981 aattagacaa aatatatgat aaaataattt ttggaataga aaataattta aaattagaag
    14041 atgaaaaatt agtaaaaatt ggagcattac tccataattt aagtccatta gctactatgg
    14101 atagaggtta tagtataact caaaaaaatg ggaaagtaat aaatagtata aaaggattaa
    14161 aaataaagga tagtatagat atagtattaa aagatggaaa tttagaatgt atgatagata
    14221 aaattgaaaa taaagaggga tagtatgaat ttaacctacg aagaagctta taaaagatta
    14281 gaaagtattt taagtgaatt agaatctaaa aatgctagct tggatgaatc tttaagccta
    14341 tatgaagaag gtataagcct ttataaacat tgtaataagc ttttagatga tgctaaattg
    14401 aaaataagta aatttaatca attgggaata gaagaagact ttaaaataga ggaggaataa
    14461 aattggaatt taaacaatgt ctaaaagaaa aagcaagttt tgtagaaaaa gtacttaaag
    14521 aatatatgcc taaagaagaa gggtatcaaa aaactgttat tgaagctatg aactatagct
    14581 taagtgctgg aggtaaaaga ttaagaccaa tacttacatt ggaggcttgt aaaatagttg
    14641 gaggtaatga agatgaagct attccttttg ctattgcaat tgaaatgata catacatatt
    14701 cactaataca tgatgatttg ccagctcttg acaatgatga tttaagaaga ggaagaccaa
    14761 caaatcataa agtatatgga gaagctatgg ggattttagc aggagatgct ttgttaaact
    14821 atgcttttga agtaatgctt gcaggctcaa tcaataaaga aaatccagaa aagtatctaa
    14881 aagctataaa tgaaattgcc aaaggtgctg gtatatatgg aatgataggt ggtcaagtag
    14941 tagatgtaga aagcgaaaat aaacaaattg aaaaagaaaa attagattat atacatatga
    15001 ataaaactgc tgcaatgatg gtaggctgta tgagagcagg agctactata ggtggagcaa
    15061 attctgaaca aatggaagaa ataactaaat atgcaaaaaa tatcggatta tcattccaga
    15121 tagtagatga tatactagac atagtaggag atgaagctaa gttaggtaaa aaggttggaa
    15181 gtgatataga aaatcataag tcaacatacc catctctttt aggtttagat aaatctaaag
    15241 aaattgcaca taatttgatt gatgaagcaa agaaaagtat agaaaaatta tctgatgatg
    15301 tagatttttt aaagggattg gctgaatata taatagatag agaatactaa aatatacagc
    15361 taaaatcgag gagggaaaaa tggatttttt ttcggagatt tttaacaatg gggctttggg
    15421 aataagtctg attgcttgtt tcttagcaca atttataaaa atatttactg gtaaggaaaa
    15481 aagaattgag ttatcaagaa tacttatttc aggaggtatg cctagttccc atagttcttt
    15541 tgttacaagt ttagctacag tggtaggtat tgaaaaaggg tttaattcta ctgattttgc
    15601 tataattacc gtgcttgcct taattataat gtatgatgca gcaggtgtta gacgggctgt
    15661 tggtaaacaa gctactatat taaatcaaat ggtggcagat atacagcatg gaaagcatat
    15721 agagcagaag aaattaaaag aattaatagg tcatacacca ctagaagttt ggtttggagc
    15781 tttacttgga atagtaacag ctttaatatt gatgtaaatt gaaaatttac agaataaagg
    15841 tgaggataat atgtataaat atttagataa agtaaattct ccaaaggata taaaaaatat
    15901 gagtatagaa gaaatggatt tacttgcaaa ggatataaga aagttcttag taaaatctgt
    15961 ttcaaagaca ggaggacact tagcttctaa tctaggagtg gttgagttaa cattagcact
    16021 tcataaagta tttgatagtc ctaaagataa gattgtatgg gatgtagggc atcaatctta
    16081 tgttcataaa atagttactg gaagaaaaga ttgttttgta tctttgagac agtttaatgg
    16141 actaagtggt tttccaaagg agaatgaaag tcctcatgat atttttgata caggacatag
    16201 cagtacatct atttctatag ctacaggtat tgcctgtgct agggatataa aaaaagaaaa
    16261 ctatagtgtt atatctgtga taggagatgg ttctataaca ggaggtatgg cacttgaagc
    16321 tttaaatcaa ttaggatata tagataccaa tatgatagta atccttaatg ataatgagat
    16381 gtccatagat aaaaatgttg gaggaatgtc taaatatctg tcaagtatta taagaaactc
    16441 aacagttgag aaaatgacag atgaagttga taaaatttta aatgttacac aaacaggtga
    16501 aattttatct aagactgcac atagatttaa agataaacta atgtatagct tttctcctca
    16561 ggactgttca ttttttgatt cattaggtat aagatattat ggacctatag atggccataa
    16621 tactaaagaa ctaatagata tattgagaaa agctaaacac aaaaaagggc ctgttctttt
    16681 acatgtaatt acgaaaaaag gaaaagggta cagatttgca gaagaacaac ctgataaata
    16741 tcatggagta tcaaagtttg atataaagac aggtgttaca tctgcaaaag ttaagtctat
    16801 gtcaattagt gttggagaaa aacttgttga tatggctaat agtaatgaaa atatagtagc
    16861 aataacagct gcaatgccat ctggtacagg gctaaacctg tttgaaagtg catatccaaa
    16921 aagatattat gatgttggta tagctgaaca acatgcaaca ggatttgcag caggtcttgc
    16981 aaaaaatggt atgaaacctt actttgctgt gtactcatca tttttgcaaa gggcatatga
    17041 ccaagttatt catgatgtat gcataacaaa aaagccagtt acttttctta tagatagagc
    17101 tggattagtt ggaaatgatg gagaaactca tcatggtatg tttgatttaa gttacttaaa
    17161 ttctattcca aatattgtag taatggctcc aaaagacaca agagagatgg agcttatgat
    17221 ggatttgtca ttaaaattag attgtccact agcaattaga tacccaagag gaagtagtta
    17281 ttacctagat aagggagagt atggagagat tgtactgggt aaatatgagg tattagatga
    17341 gggtcaagat acagtcatac tttgtattgg aagtatggta aaacatgctt tagaggctaa
    17401 agaaatatta tcaagagaag gaataaatcc gacaatagtt aatgctagat tcttaaagcc
    17461 aattgatgaa ggtatgttaa aagcattatt aaaaaatcat aaaaatgtag ttactataga
    17521 ggataatatt gtaactggag gatttggaag tagaataaat aagtttatta tagataatga
    17581 atataacgta aatatactta atatagcaat accagaagaa tttgtaaaac atggcaatat
    17641 agatgaatta tatgactttg taggattatc gccaaaaagt attgctgata agataaggaa
    17701 attagttatt gagtaaaaat ctaatgtaga tattgtaaat taaaatttat ataaaatata
    17761 gaatttatca attagaataa atatcaagtg tgttacttca agctacttta gttatcatta
    17821 aagtagcttt taacagaaag gtaattgtat gaaaaaaaga atagatttat tattagtaga
    17881 acaagggtac tttgaaagta gagaaagagc caaaaaagca ataatggctg gattggtttt
    17941 tgttgataat caaaggtgtg ataaggctgg tacagaagta aaagaagatt gtagtataga
    18001 agtaaaagga aatccaatac catatgtcag tagaggtggt ttaaaacttg aaaaagcaat
    18061 gaaaaacttt gatttgacta ttgatggaaa ggtatgtatg gatatagggg catccacagg
    18121 tggttttaca gattgtatgc tcaaaaatgg agcaataaaa gtattttcta tagatgttgg
    18181 ttatggtcaa cttgcatgga aattgagaca agatgatagg gttgtgtgta tggaaagaac
    18241 caatataaga aatgtgacaa tagaagatac aaagcagttt gctgattttg cgtcaattga
    18301 tgtgtctttt atatcgttaa agttagtact tccaaaggca aaggagttag tacgtcatga
    18361 tggggaagtt gtcgctttaa ttaaacctca atttgaagca ggaagagaaa aagtaggtaa
    18421 aaaaggagta gttagagaaa aatctacaca tatagaagtt ataaaaatga tttctgattt
    18481 ctcagtcgaa aatggatttg agattttagg gttagatttt tctcctatta aaggtcctga
    18541 aggtaatata gaatatctaa ttcatttgag aaatggaaat gaaggatatg aatttgatgg
    18601 tgagacatac aataacaaaa tagtagaagt tgttgaagct tctcataatt tagacaaata
    18661 ggttaaaagg gggcttagat ttgatcctcg aattatatat gaaaaattgt gccttagttg
    18721 aagaactaag gttgaatatt gataaaaact taaatatact tacaggtgaa actggttcag
    18781 gaaaatctat aataatagat gcacttggac tttgtttagg tgataaatat gataggtctt
    18841 ttttaagaaa aggaacagat aagggacttg ttgaggctgt atttttttca gacaatagat
    18901 atcttaaaaa aatattagaa gaaaatgata taagtatgga agatgataat ttattggtaa
    18961 taactaggtt gatatactca gatggtaaga gtactgctag agtaaatgga aggactgtta
    19021 aagtgtctct tcttaaagaa attgcatcta cacttataga tatacatggt caacatcaaa
    19081 atcaagcttt gtttaataaa gatactcatt taaaatttct agaccttttt ggagaaaatg
    19141 aattggaaga gtttaaaatt gcttataaaa aggtgtacca taagtatagt gaagtaaaaa
    19201 aagctttaaa ttgcttgaca gaaaataaag atgagatgca aattcaacga gaaatagatt
    19261 tattaagatt tcaaataaat gaaattgaag ctgcaaatct aaataagaat gaatatgaag
    19321 atttattaaa acaaagagaa gtttatagaa atagtgaaaa gatatataat aatctaaatt
    19381 cgagttatag caagttacat aatggtgagt ataatgttat tgacctaata ggtcttgcat
    19441 ctaaggaact taatgacata tctaaatatg acagtgtttt aagtgaatat agtgatactg
    19501 tagaaagaat catgtatgag ttacaagaca tatctggtga gataagaaat tacaaagata
    19561 atatagactt tgaaccttat gagctagagc agattgagct tagaatcgac gagattaata
    19621 acttaagaag aaaatatgga gacagtatag atgatatatt tgagtattat ggaaaaatta
    19681 aagatagatt agatgaaata ttaaacagag atgaaagagt tgaacagtta agaagcaaac
    19741 tgatgaatat agaagaagat ttaaaaataa aagccagtaa attgactaaa gcaagaatag
    19801 aagtagcaac tactttagaa aaaatacttc ttgatgaatt aaaaagctta aatatgaaaa
    19861 atgtaatgtt caaggttaat tttgaaaaag gtccatttac tttagatgga gtagatgata
    19921 ttgaatttat gatatctttt aacttaggag aagatattaa gcctatatat aaagttgcat
    19981 caggtggtga gatgtccaga tttatgttag cgtttaaaac catattggct gatatagatg
    20041 atattgatac attagtgttt gacgagatag atactggaat aagtgggata gcagcacaga
    20101 tagttggtga gaaattgagt gatatagcaa agaaaaaaca gataatatgt ataactcatt
    20161 tacctcaaat tgctgcaaat gcagacactc attattgtat agagaaagat acttcaaata
    20221 ataggacgtt taccaatgtt agtaagttaa atgaatctca acgaaaaaat gaaattgcca
    20281 gacttatagc aggtaataat ataacagaaa aaactattga acatgcaagt gaaatcattg
    20341 aattggctaa aaaatgttag tgaaaaacaa gcattttaat aggtgtaaag aatttttata
    20401 gaaatgcttt acacctattt tttataatga aattatttta aaatatctag tgcctatatt
    20461 gaagaaaagt aggtttgaaa atggaataaa atttgtataa atttgtaact ttgaaatttc
    20521 taaatgctta agtataatat tatttattaa ggtagtagtg aggggaggca taaaatgaat
    20581 aaaaaagata ctatgcactt aatttttata attgtaattt ttattttatt atggtatatt
    20641 gtggtagcag taagtgagat taataaaaat attgaaagtt gtagtaacaa attagattat
    20701 ttgtcacaag agcttatgag tactaagtcg gatattagta atactataaa tgaagaaatg
    20761 tcaaaaagtt atataacaaa aagtattgac ttaaaagtaa aaaaaatcga gaaaaaagaa
    20821 tgtattattg atgtagatgt tcaattaagt aaacttggaa aaaatggaaa agtatttttt
    20881 atgtataaag aagatagtga taaatggaat gaaactgaga tgacaaaata tggagaatta
    20941 tcatatgcat gtgaaataaa gataaataca ggaagtgaat atgactataa agtagttaca
    21001 agtggagcta tatctgaaag ttctgatgtg caaaaattga ctaaatctga ttatatgcct
    21061 gaacaaccca tatttaattc tgggataaga gataatagag aatactttat tgaaatagta
    21121 gaaaatagta atcttttcaa tcatgagagt gaaaagtcaa aaaataaagt ttatgataat
    21181 attagattag aaaaagtaga tattattgta aatgaaggaa aaaaagacac tatatacaaa
    21241 gccaaattag ctgaaggctt agatgatggc aaaactaata attcaaatag aaatcaagtg
    21301 aattatgaag taagttttcc taaaagggat attgatgaca taatttatat taaagctaaa
    21361 cttacataca ataatggagt agaatataca aaggatataa ctgaagatat aactatggga
    21421 ttacaagatt tagaaaaata aatacatatt taaataaata ttcaaataga tttgttcaat
    21481 aaaatagagt ataaataaaa ctgtagtagt tttacttata ctctattaaa aaattataat
    21541 tttaattcca tatatctagc cccttcaatt ggattataag tataaggttc tatatcataa
    21601 aaaccaaact tttcatataa tccttgtgca cttttcatgc ttggaagagt atctaatctc
    21661 atgtgtgtat atccaaattt tttagcttct tcaatgattt cttccaatag tatttttcct
    21721 atttttaatc ctctaaattg atttcttaca tataatcttt ttaattcaca tattttacct
    21781 tcaagttttt ttaaagcaac acaacctgcc aaattttcat caacaaatgc caatatcaat
    21841 gagcctgaag gttttttata ttttcctgga agtgttttta attcattatt aaagtcttga
    21901 aaacataagt ctatatttag agaatttgaa tattcagtaa atagaagttt tacgttttca
    21961 aggttttcta aatctttttc ttcaacaaat tgtaaagtta tattttgagc cattttatcc
    22021 gccattatat acacatgttt atataatgac ttcacctcct tgatatatgt atatttttct
    22081 taatatcaat tagattttaa taccattact ttttcaccac catagtcaag ctctccatta
    22141 aactcaaatc caaaatcttt ataaagctta atagccattt tattgtcttc gaatatacta
    22201 agatatagtt catcacagtt atattcattt ttaagatgat ttacaagaaa atctaatgat
    22261 gcttttccat atcctttacc ttgatatttt gaatcaatca taaatctatc aagccaaact
    22321 ctatcatctt ctaaacgacc atacatagca aatcctatta aagaattttc atcatatatt
    22381 ccaacaggta cccaagctgt tataaattta gattcagcaa tagaaagtgc attgctttct
    22441 atataatcta actgactttt atctacagac aaactaacta aatcacgcca attatttatg
    22501 tctgcaattc tcacatttaa tttcaagata ttaccgccct tcaaactttt tgttacaatc
    22561 taatactatc atattatgta ttaaaaatca cttattaaga ttttaaataa actttttaaa
    22621 tgaataaatt ctcaggaaaa aggaattata aatacataat aatacagcat aggagtagat
    22681 ggatatatga acaaaataaa taaaaatttc aaaaatattt ggataatatt aggagctgta
    22741 tcgattttta ccatggttat aatttttaat aaaaattgta atgttaataa agagactcaa
    22801 atagaagctt ttacaattaa gaatcatgat aaaaaatttg tatatcctat gggaaatata
    22861 attggagtaa aagcaaatac agatggtgta ttggtcttag gatatgagga agaggatgta
    22921 gactatattg gaggcataca aatcggtgat aatatagtaa aaatagataa taaaaggata
    22981 aaaaatagtc aagatgtatc agagattttg aataagatta aaaaaagtaa agttgaagtg
    23041 acttttgaga gaaaaaataa gtataaaaca gaagtaatag agactaaaaa agaaaatggc
    23101 aagtataaac ttggattatg ggtaagagat aaaatatctg gaattggaac tatgactttt
    23161 tacgacccta gtatggaaaa gttcaaagca atcggtcatg ccataaaaga ttcggatact
    23221 aatgaactat taaagataaa gcaaggatat atatataagc cagaacaatt aaaaatagta
    23281 aaagctagta atgaaaaagt aggtaaaata aaaggagatt ttaatgacag caatttaatg
    23341 ggtaatttct caaataattc agagttaggt ataagtggta atattacaga aaaccataat
    23401 aaagagttta atgtagcaaa taaagaaaag caattaatag aagttggaag accgcaagat
    23461 gtaaaaatag gagatgcagt aattcttttt gaggataaaa acaaaaatat aacaagctat
    23521 gatataaaaa tagaaagtat agtatatgat aaaggaaatt atagagatat ggtaataaaa
    23581 gtagtagatg acaagttatt ggaatacaca ggaggtatcg tacaggggat gagtggagct
    23641 ccaataatac aaaataataa aattattggt gcaataactc atgtttttag agataatccg
    23701 aaaaaaggat atggtatttt tatagatgaa atgataaaat tgtaggtgag gcattaaaaa
    23761 attttattat tttatcaatt atctaggagg aatataattt tggagtgtcg aatatgcttt
    23821 agagtagata attaggaagc aattgtgtaa aaagtttagt tttctgtaat aagaagatgt
    23881 tttttaatgg ggggattttt agtggaaaaa atcaaaatag ttttagcaga tgacaataag
    23941 gatttttgtc aggtattaaa agagtatttg tctaatgaag atgatatcga tatattaggc
    24001 atagctaagg atggaattga agcattagac ttagtaaaaa agacacaacc ggacttatta
    24061 atactagatg taataatgcc acatctagat ggattaggtg taattgaaaa attgaatact
    24121 atggatatac ctaaaatgcc aaaaataata gtactatcag cagtaggtca agataagata
    24181 actcaaagcg caataaatct aggagcagat tactacatag taaagccatt tgattttgtt
    24241 gtgtttataa acagaattag agagctagtt tctaatagag ttacacaagt tgagccaaaa
    24301 cctagaccag ttcaagagac tcaaatgact agaagtgatt ttgttaagaa tgtaggaaat
    24361 atagagacag aaatcacaaa tataatacat gaaataggag taccagctca tataaaagga
    24421 tatttatatt taagagaagc aataaaaatg gttattgata atgttgagct tttaggtgca
    24481 gtaacaaaag agttatatcc aagtatagct aaaaaattca atacaacacc aagtagagtt
    24541 gaaagagcaa taagacatgc aatagaggtt gcatggagta gaggaaaagt tgacacaata
    24601 aatcaattat ttggatatac ggtacacaat actaaaggaa aaccaactaa ttcagaattt
    24661 atagcaatga ttgctgataa attaagacta gaacatagta tggttaaata aacaagacat
    24721 aaaaagtaag gctttttaat taaggcattg gctataaatg cgtattacaa gcagcgaaac
    24781 g
//
LOCUS       DAELJO010000038        24685 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELJO010000038 DAELJO010000000
VERSION     DAELJO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.038"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..165
                     /locus_tag="KPB88_001359"
     rRNA            <1..165
                     /locus_tag="KPB88_001359"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            293..409
                     /gene="rrf"
                     /locus_tag="KPB88_001360"
     rRNA            293..409
                     /gene="rrf"
                     /locus_tag="KPB88_001360"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            529..720
                     /locus_tag="KPB88_001361"
     CDS             529..720
                     /locus_tag="KPB88_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG0053654.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            875..2284
                     /locus_tag="KPB88_001362"
     CDS             875..2284
                     /locus_tag="KPB88_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0053655.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLNAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYNTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2415..3104
                     /locus_tag="KPB88_001363"
     CDS             2415..3104
                     /locus_tag="KPB88_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG0053656.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            3107..4042
                     /locus_tag="KPB88_001364"
     CDS             3107..4042
                     /locus_tag="KPB88_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG0053657.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            4039..4932
                     /locus_tag="KPB88_001365"
     CDS             4039..4932
                     /locus_tag="KPB88_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG0053658.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            5010..5756
                     /locus_tag="KPB88_001366"
     CDS             5010..5756
                     /locus_tag="KPB88_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG0053659.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5757..6590
                     /gene="rsmI"
                     /locus_tag="KPB88_001367"
     CDS             5757..6590
                     /gene="rsmI"
                     /locus_tag="KPB88_001367"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG0053660.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6613..7317
                     /locus_tag="KPB88_001368"
     CDS             6613..7317
                     /locus_tag="KPB88_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG0053661.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7505..7933)
                     /locus_tag="KPB88_001369"
     CDS             complement(7505..7933)
                     /locus_tag="KPB88_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0053662.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7970..9400)
                     /locus_tag="KPB88_001370"
     CDS             complement(7970..9400)
                     /locus_tag="KPB88_001370"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG0053663.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9532..10116
                     /locus_tag="KPB88_001371"
     CDS             9532..10116
                     /locus_tag="KPB88_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG0053664.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            10132..10653
                     /locus_tag="KPB88_001372"
     CDS             10132..10653
                     /locus_tag="KPB88_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBG0053665.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10719..10965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11049..12986
                     /gene="metG"
                     /locus_tag="KPB88_001373"
     CDS             11049..12986
                     /gene="metG"
                     /locus_tag="KPB88_001373"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG0053666.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKGELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            13016..13786
                     /locus_tag="KPB88_001374"
     CDS             13016..13786
                     /locus_tag="KPB88_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG0053667.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            14124..14555
                     /gene="phnG"
                     /locus_tag="KPB88_001375"
     CDS             14124..14555
                     /gene="phnG"
                     /locus_tag="KPB88_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG0053668.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14570..15145
                     /gene="phnH"
                     /locus_tag="KPB88_001376"
     CDS             14570..15145
                     /gene="phnH"
                     /locus_tag="KPB88_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG0053669.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            15148..16233
                     /locus_tag="KPB88_001377"
                     /pseudo
     CDS             15148..16233
                     /locus_tag="KPB88_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
     gene            16253..17122
                     /locus_tag="KPB88_001378"
     CDS             16253..17122
                     /locus_tag="KPB88_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG0053670.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            17115..17981
                     /locus_tag="KPB88_001379"
     CDS             17115..17981
                     /locus_tag="KPB88_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0053671.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            18022..18735
                     /locus_tag="KPB88_001380"
     CDS             18022..18735
                     /locus_tag="KPB88_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0053672.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18736..19905
                     /locus_tag="KPB88_001381"
     CDS             18736..19905
                     /locus_tag="KPB88_001381"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG0053673.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPSNGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19909..20745
                     /locus_tag="KPB88_001382"
     CDS             19909..20745
                     /locus_tag="KPB88_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0053674.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20746..21741
                     /locus_tag="KPB88_001383"
     CDS             20746..21741
                     /locus_tag="KPB88_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0053675.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21759..23447
                     /locus_tag="KPB88_001384"
     CDS             21759..23447
                     /locus_tag="KPB88_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0053676.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23459..24526
                     /locus_tag="KPB88_001385"
     CDS             23459..24526
                     /locus_tag="KPB88_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0053677.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDHGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9822 a         2786 c         4422 g         7655 t
ORIGIN      
        1 ggaatggata agcgctgaaa gcatctaagc gcgaagccaa cttcaagata agatttccca
       61 ccgcaagggt aagaccccag aaagactatc tggttgatag gtcgaaggtg taagtgcagc
      121 aatgtattta gcttatcgat actaataggt cgaggacttg accaatattt atttgatgtt
      181 cattcattaa gatatatgtg tagtttttag agtgctaact ctaaagaaac tagtattact
      241 agattctata aacttattaa aaacttaata gaaatattaa gcataaagat tatgtggtta
      301 caatagcaga gaggatacac ctgttcccat tccgaacaca gaagttaagc tctctagcgc
      361 tgatggtact tggtgggaaa ctgcctggga gagtaggacg tagccacgta atttttttta
      421 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
      481 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      541 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      601 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      661 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      721 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      781 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      841 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      901 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      961 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
     1021 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
     1081 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
     1141 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
     1201 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
     1261 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
     1321 aatagataaa aacttgaatg caaaagcagt actgcttact tatcctacat attatggaat
     1381 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1441 agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa cagctcttga
     1501 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1561 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1621 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1681 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1741 caaaaataaa gttgattcaa atattaatat atacaacaca aatgataaaa caaaaatatt
     1801 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1861 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1921 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1981 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     2041 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     2101 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     2161 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     2221 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     2281 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     2341 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     2401 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2461 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2521 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2581 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2641 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2701 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2761 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2821 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2881 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2941 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     3001 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     3061 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     3121 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     3181 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     3241 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     3301 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     3361 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3421 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3481 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3541 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3601 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3661 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3721 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3781 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3841 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3901 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3961 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     4021 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     4081 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     4141 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     4201 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     4261 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     4321 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     4381 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4441 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4501 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4561 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4621 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4681 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4741 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4801 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4861 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4921 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4981 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     5041 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     5101 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     5161 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     5221 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     5281 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     5341 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     5401 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5461 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5521 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5581 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5641 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5701 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5761 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5821 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5881 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5941 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     6001 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     6061 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     6121 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     6181 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     6241 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     6301 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     6361 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6421 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6481 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6541 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6601 tgaggtgatt ttatggaaat aggaaaaatt ataatagaga gtttaaagag ggaagataca
     6661 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6721 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6781 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6841 ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat aaataagtta
     6901 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6961 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     7021 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     7081 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     7141 attaatgtat ttaacttagt agatgaggat actataggtg taataatgct tgggtatgca
     7201 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     7261 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     7321 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     7381 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7441 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7501 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7561 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7621 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7681 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7741 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7801 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7861 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7921 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7981 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     8041 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     8101 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     8161 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     8221 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     8281 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     8341 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     8401 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8461 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8521 attcgtctag gtcattagat gcagtgatgc tgatatctga aaatccagcg ttatctaatt
     8581 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8641 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8701 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8761 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8821 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8881 ctacagcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8941 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     9001 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     9061 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     9121 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     9181 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     9241 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     9301 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     9361 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9421 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9481 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9541 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9601 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9661 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9721 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9781 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9841 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9901 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9961 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
    10021 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
    10081 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
    10141 ttttcaaact tacttatacc aataataatt ctgtacatag taatttatgg taaatataaa
    10201 aaaatagatg tgtatgatag ttttgtaaaa ggagcgattg atggattaaa agctgcatgg
    10261 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
    10321 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
    10381 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10441 agtgaacttg caaatagagt tgggatagga agtttagtag aaaaaatggg tgctacgatt
    10501 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10561 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10621 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10681 taatatagtg attatataga aacaaatagt aatataaatt acaagctatg aaaagaagag
    10741 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10801 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10861 acgttatagt ctactaaggt atctataaaa tatttctaca tttcatgtga aacataacta
    10921 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10981 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa atttaggagg
    11041 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    11101 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    11161 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    11221 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    11281 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    11341 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    11401 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11461 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11521 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11581 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11641 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11701 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11761 taggttatat gactgataat gatgaagaat tcaaaaaata ttggcctgcc aatgttcaaa
    11821 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11881 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11941 aaatgagtaa atcaaagggt aatgtggttt atccagaacc tataatagaa agatatggaa
    12001 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    12061 ctcatagaaa ttttgtaaca agaataaatt atgatttagc aaatgattta ggaaacttaa
    12121 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    12181 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    12241 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    12301 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    12361 agaaaggtga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12421 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12481 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12541 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12601 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12661 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12721 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12781 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12841 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12901 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12961 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    13021 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    13081 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    13141 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    13201 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    13261 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    13321 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    13381 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13441 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13501 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13561 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13621 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttccca
    13681 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13741 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13801 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13861 tttagttcat gaggcactct tttttttatg aaatcaaata tattttcatc atttcaacaa
    13921 tgaaaaagaa gtttatcata ttttgagata gtttgttttt atttatttaa ttcatatttt
    13981 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    14041 caactctaat attcacttaa cttaagttgt ttattatatt attaaagaga agtaaacgaa
    14101 aggtagatta aggaggttga attttgtata cagttgatag tactaagata ttgataaata
    14161 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    14221 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaaca
    14281 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    14341 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttattta gcagttatag
    14401 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14461 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14521 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14581 tacacaagaa atatatagaa agatgctaga tagtgcttca tttcctggga aattaaataa
    14641 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14701 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14761 aaaaaatata agagcactta caatgtcaaa tcaaatagat tatagaaaat cagattatat
    14821 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14881 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14941 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    15001 aaatggaatt agagaagaat ttatagaaag attatctagc ataaataaag attatccaat
    15061 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    15121 aaaattaagt tgggaggtat tatagaaatg gcttatgtag cagttaaagg tggagaagaa
    15181 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    15241 atagatgtaa agtagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    15301 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    15361 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15421 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15481 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attgaatgta
    15541 gaattattag aagaagatga aaaaaagaat agacatttta gagatagttt tataaatgaa
    15601 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15661 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15721 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt aactagaggt
    15781 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15841 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15901 gaagatgaag aaataataat aggggatatg ctggttactg aatgtgaaat agtttcaaaa
    15961 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    16021 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    16081 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    16141 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    16201 gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt aggtgatata
    16261 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    16321 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    16381 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16441 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16501 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16561 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16621 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16681 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16741 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16801 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16861 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16921 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16981 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    17041 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    17101 aggaggtact tggaatgagt aaaataaatg actctaaaag attagaaaat atagacaagc
    17161 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    17221 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    17281 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    17341 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    17401 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17461 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17521 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17581 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17641 gaatagacca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17701 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17761 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17821 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17881 taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg gaggacccac
    17941 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    18001 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    18061 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    18121 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    18181 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    18241 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    18301 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    18361 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18421 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18481 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18541 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18601 gaaaaaatgt tggagcttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18661 gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac tataagtgaa
    18721 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18781 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18841 aagattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18901 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagcca
    18961 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    19021 tcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    19081 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    19141 aagagcataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    19201 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    19261 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    19321 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    19381 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19441 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19501 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaagaaatt gggtgttcta
    19561 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19621 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19681 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19741 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19801 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggttgta
    19861 aatactattg taaatggaaa aacagtttat aaaagggagg tataaagatt gctaacagag
    19921 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19981 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    20041 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    20101 tcagcatatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    20161 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    20221 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    20281 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    20341 gggtatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    20401 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20461 tctggtggaa tttgtgtgct agctcaccca ggtcaaatgg ataattttag cgctattgaa
    20521 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20581 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20641 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20701 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20761 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20821 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa gagtacacta
    20881 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20941 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    21001 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    21061 agtaaatcta atattgataa aaaagttaaa gaagttctag aagttgtaga attaagtgga
    21121 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    21181 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    21241 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    21301 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    21361 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21421 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21481 aagatagcta ttagaccaga agatataaaa gtagagtcta acagagattc taaagataag
    21541 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21601 gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21661 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21721 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21781 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21841 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21901 gaaattttat aggaatttca aattttagta aatatgttga aaatccaagt cttatagcat
    21961 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    22021 ttatatattc atacgcaata acaagaacaa acataaagtt taaagatttt tttaaaacta
    22081 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    22141 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    22201 gaataattat ttctcaaatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    22261 cactttcgat ggctgattac agactttatg aagcagcaga tagcttgggt gctagtgaat
    22321 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    22381 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtt ggaggaaact
    22441 acaatgtact ggccactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22501 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22561 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22621 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22681 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22741 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22801 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22861 gtgcttatat gatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22921 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22981 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    23041 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    23101 tggataaaga gtatgaatta gttgcaaaat ccataaatat tccattctat aaagtatttt
    23161 ttaatataac agttccaatg tctataacat caatacttga aatagtagta tattattttg
    23221 taaattcaat ggttactgtt tcagcattaa tatttttata tacacctcaa actcagactg
    23281 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    23341 cagtattagt gcttttaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    23401 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggtaaaaat
    23461 gaaatttaag aagctagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23521 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23581 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23641 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23701 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23761 aagcttattg gttgcagatg accacgggat gttagaacca tatgctccaa aaggttctga
    23821 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23881 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23941 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    24001 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    24061 aaaagcatgg cagtatatgg ataagttaca tgagaatatt ggtgtataca ctcaatctgg
    24121 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    24181 tagaggtata aagttaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    24241 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    24301 aaaagtattt ttagattggg ctataagtaa agatgcaatg aacgaatact ctaaaaacta
    24361 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24421 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24481 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24541 atggagtaaa aagaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24601 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24661 tttgaatggt atatttttta ttttt
//
LOCUS       DAELJO010000039        24046 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELJO010000039 DAELJO010000000
VERSION     DAELJO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.039"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..334
                     /locus_tag="KPB88_001386"
     CDS             <1..334
                     /locus_tag="KPB88_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0053678.1"
                     /translation="KTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEIDLDMVNIRET
                     FNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLILNNLKNL
                     EKQYNSSY"
     gene            347..859
                     /locus_tag="KPB88_001387"
     CDS             347..859
                     /locus_tag="KPB88_001387"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG0053679.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            933..3140
                     /locus_tag="KPB88_001388"
     CDS             933..3140
                     /locus_tag="KPB88_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG0053680.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3165..3614
                     /locus_tag="KPB88_001389"
     CDS             3165..3614
                     /locus_tag="KPB88_001389"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG0053681.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3629..4243
                     /locus_tag="KPB88_001390"
     CDS             3629..4243
                     /locus_tag="KPB88_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0053682.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4334..5890
                     /locus_tag="KPB88_001391"
     CDS             4334..5890
                     /locus_tag="KPB88_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG0053683.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNADNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5880..7142
                     /locus_tag="KPB88_001392"
     CDS             5880..7142
                     /locus_tag="KPB88_001392"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG0053684.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7216..9003
                     /gene="aspS"
                     /locus_tag="KPB88_001393"
     CDS             7216..9003
                     /gene="aspS"
                     /locus_tag="KPB88_001393"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG0053685.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9462..10694
                     /locus_tag="KPB88_001394"
     CDS             9462..10694
                     /locus_tag="KPB88_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0053686.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10708..11514
                     /locus_tag="KPB88_001395"
     CDS             10708..11514
                     /locus_tag="KPB88_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG0053687.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11626..12495)
                     /locus_tag="KPB88_001396"
     CDS             complement(11626..12495)
                     /locus_tag="KPB88_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0053688.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12867..14294
                     /locus_tag="KPB88_001397"
     CDS             12867..14294
                     /locus_tag="KPB88_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG0053689.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14371..14640)
                     /locus_tag="KPB88_001398"
     CDS             complement(14371..14640)
                     /locus_tag="KPB88_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053690.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14643..16085)
                     /gene="nhaC"
                     /locus_tag="KPB88_001399"
     CDS             complement(14643..16085)
                     /gene="nhaC"
                     /locus_tag="KPB88_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0053691.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16110..17276)
                     /locus_tag="KPB88_001400"
     CDS             complement(16110..17276)
                     /locus_tag="KPB88_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0053692.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVVLPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(17501..19621)
                     /locus_tag="KPB88_001401"
     CDS             complement(17501..19621)
                     /locus_tag="KPB88_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0053693.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTLF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANQMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPTDIFEIFGSLKYDIPCKFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19854..20255
                     /locus_tag="KPB88_001402"
     CDS             19854..20255
                     /locus_tag="KPB88_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053694.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            20298..20591
                     /locus_tag="KPB88_001403"
     CDS             20298..20591
                     /locus_tag="KPB88_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG0053695.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20606..21046
                     /locus_tag="KPB88_001404"
     CDS             20606..21046
                     /locus_tag="KPB88_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG0053696.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21138..21611)
                     /locus_tag="KPB88_001405"
     CDS             complement(21138..21611)
                     /locus_tag="KPB88_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0053697.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21611..22399)
                     /locus_tag="KPB88_001406"
     CDS             complement(21611..22399)
                     /locus_tag="KPB88_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0053698.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22727..23971
                     /locus_tag="KPB88_001407"
     CDS             22727..23971
                     /locus_tag="KPB88_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0053699.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
BASE COUNT         9181 a         2945 c         4056 g         7864 t
ORIGIN      
        1 taaaacagac ctattatatt taaagaatgt agttagtaat atagttccat caagagatga
       61 atttataaca atttataaac aagcactcat aaaaaaagaa attgatttgg atatggtaaa
      121 tataagggaa acttttaatg taattccatt aaagtttttt acgatattaa atgtatttag
      181 agaattaaat ttacttgact ttaatttaaa ttatgaaaaa aattctgtac tcataagaat
      241 attgccaaaa ccacagaaaa aattagactt aaatgagagt ttaatactca ataatctaaa
      301 aaatttagaa aaacaataca atagcagtta ttaggaggcg tttcccatgg atttaaaaaa
      361 ctttataagg aatattgatg atttcccaaa gccaggcata gattttaaag atgtgacaac
      421 tctatttaaa gatggagatg catttaaata tgcagtggat tctatagttg aagaattgaa
      481 agataaagat gtagatttag taataggacc agaagcaaga ggatttttga tgggtacacc
      541 tgtagcatat gctttaggag taggatttgt tccaattaga aaacctggaa agttaccagg
      601 tgaagttgag agttatgagt atggattaga gtatggtaca gatactttag agatacataa
      661 ggatgcaatt aaaaagggtc aaaaagtagc aatagtagat gatttattgg ctactggtgg
      721 aactatggaa gccgctgcca agcttgtaga aaaattaggt ggagaagtag tttctatgca
      781 atttttgatt gagctaaaat tcttaaatgg gagagaaaag ttgtctaact atgatgttaa
      841 ttctcttata aaatactagt acttttataa tagttggtat aacaccaact attttcatat
      901 atattaaaat tttatacaaa ataggtggtt atatgcatga taaagaatta caagaaataa
      961 ttgaaaaaat aaaagtttat gcacctaatg caaattttga aatggttgaa aaagcctata
     1021 atcttgcaaa aaaagcgcat gaaggacaat atagaaaatc tggggaacca tactttattc
     1081 atccattagc agttgcaaat attttaatag agatggaatt agatattgaa actataacag
     1141 caggtttact tcatgatgtt gtagaagata cagaatatac ctatgaagac ataaaaaaag
     1201 aatttggcaa agaagtagcg gatttggttg atggagtaac taaacttggt caaataaaat
     1261 atcgttcgaa agaagaaact caatctgaaa atttaagaaa aatgtttttg gcaatggcga
     1321 aggatataag ggttatactt ataaaacttg ctgatagatt gcacaacatg agaacattaa
     1381 aatacatgcc tccagaaaaa gcaaagtata aagcaaaaga gacacttgaa atttatggtg
     1441 gtatagctca tagattagga atttccaaga taaagtggga actagaagac agagcattaa
     1501 gatttatgga cccagaaggg tattatgatt tagttagtag agtatcaatg aagagaagtc
     1561 aaagagagga ttatattcaa ggaattgttg aacttcttaa agataagttt aaagatttaa
     1621 gtataccatg tgatgtatat ggtagaccaa agcattttta cagtatatat agaaagatgc
     1681 aaaaaaaaca taagactttt gaagagattt ttgatttaac agcagtaagg atattggttg
     1741 ataatgtaaa ggattgctat gcagtacttg gtatagttca tacattatgg agacctattc
     1801 caggaagatt taaagattat atagcaatgc caaaacccaa tatgtatcaa tcattacaca
     1861 ccactgttgt aggtcctgat ggagaacctt tagagataca aataagaact catgagatgc
     1921 ataatatagc tgaaaatggt atagcagccc attggaaata taaagaaggt atctctagca
     1981 aagagtcaaa ggttgaagaa aaacttcaat ggttgagaca gatgatggag tgggaaaaag
     2041 atttaaaaga cccacaagag tttatggatg cacttaaaga agatgtattt aatagtcaag
     2101 tttatgtatt tacgccaaaa ggagacgtaa ttgaattacc agcagaatca acacctatag
     2161 attttgccta tagggttcat acaaatgtag gtaataaatg tgtaggagct aaaattgatg
     2221 gaagaatagt tcctattgat tataagcttc aaaatggtaa tattgtcgag gtacttacat
     2281 catctaattc taatggacca agtagggatt ggcttaatat agtgaagacc ccaaatgcaa
     2341 aaagtagaat tagacaattt ttcaagaagg aaagaagaga agaaaatgtt gaccgtggaa
     2401 atacaatatt agaaagagaa tttaagaagc atgatatacc acaaaaagac cctcttgttg
     2461 aaaaatatat ggcattagtt gctaagaaat ttaatcagcc aagtgttgag gacttaattg
     2521 ccactgtagg ttatggtggt ataatgtcat ctcaagtggt atctaaggta aaagacctat
     2581 atttaaaaga ggttaaaaaa gctaaaaagg aacaacagat acaagaagaa gatttaaata
     2641 aacacaatat aagtgaaaca gaatacagta aaaagagaaa gaaaaacgca tcacaaggaa
     2701 ttatagtaaa agggcttgac aatatcttag taagatttgc taagtgttgt aatcctcttc
     2761 caggagatga aattgtagga tatattacta agggtagggg agtagccata cacagagctg
     2821 attgcccaaa tgcaaacaat aatggtgatt tcttccaaaa tagattggtg gaggtttcat
     2881 ggagttcagc aggaaaaggt aagtttgaag cggaaattca aataaaagct attgatagaa
     2941 gaggaattgt taatgaaata actcatattg ttgcaatgga taaacttagt ttaaacggaa
     3001 taaatgcaag aaaaggtaag gatagtattg taagtataaa cttacttgta gaggttaatg
     3061 acacagagga attaaaaatt ctaatgaaaa aattaaaagc aattcctggt gtagaaagta
     3121 tttatagaat gacaaattag agttaaaata atagggagga aactatgaga gcagtagtgc
     3181 aaagagtatc ttcatctaaa gttacagtag atgaaaacac tataggtcaa ataaataagg
     3241 ggttactagt attgttagga gtgactcatg atgataaatc aagtgatgta gattatatga
     3301 ttgataaaat attgaatctg agaatttttg aagatgaaaa tgacaagatg aatttatctc
     3361 taatggatat aggtggagag cttttagtag tatctcaatt tactttatat ggagattgca
     3421 gaaaaggaag aagacctgga tttagtaatg cagctagacc agaacttgca aataaccttt
     3481 atgaagaatt tgtaaaaaaa gcaaaagata aaggtgtgac agttggtaca ggtcaatttg
     3541 cagctcatat gatggtagag ttaactaatg atggtccagt aacaatacta ttagactcaa
     3601 gtaaatcttt ttaaggggga aaactttaat gattataaaa aaatttgtag atagttattt
     3661 tggagtaaat acttatgtat taggtgatga aaagactaaa aaatgtgcag ttatagaccc
     3721 aggtggaagt ttagtagaga cattaagttt tgtaaaagaa aatgaattaa atattgaata
     3781 tattatcctt actcatgggc atggagacca tataggatat gttaaggata ttaaagaaaa
     3841 aactggtgct aaagtagttg cacatgttga tgaaaaagaa ttattaaatg acaaaaataa
     3901 aaatctaagt tatacaatga gatgtggagc acaagagttt gatgcagata tctatgtaaa
     3961 tgataaagac aagcttgaac ttggtgaatt aaaaatgact ttcttgcata ctccaggaca
     4021 tactcaagga tgtatgtgta taagagttga agatgaaatg ttcacaggag atactttgtt
     4081 tgctggaagt attggtagaa ctgatttata tagtggagac tttaaccaaa tggaaaaatc
     4141 acttaagaaa ttatgcaaat atgaagatgc tgttagagta tatcctggac atggaccaac
     4201 cagtactcta ggggttgaaa agaagacaaa tccttacatg taattttata ttagtattta
     4261 aaaaagtata aaatatttaa taactatatt agtgaggggg ttcttctact gttaaataga
     4321 aggagttaag ttgatgttgt atgttttttt aaaaggacat gattataagt atgaagtagc
     4381 agaacttata aaattgttta ccagtgaatt taagtttaaa gactctaatg ctgacaataa
     4441 tatagagtca aagcatttaa taaatagatt gatttatgag aatggtgtat tattctcaag
     4501 tactgaatac tatgaaaatg gaaacttaaa gtacgagtca atacagaata tagaaaatat
     4561 gaatcttgaa tttttagatg gatttgatag tagcaaaacg attgaagaat ttcttaatag
     4621 cctagatagt gaaagtaaga ggaactttaa aaagctgtgt aaagaaaata tcaaaaaaag
     4681 catgtttgta gtgcttaaac aagtatttaa ttcttatgta ccatggggaa ttttgactgg
     4741 tattagacca gtcaaaattg tccacaattt aatggataaa aagctagatg ataatagcat
     4801 aagaaccatt ctaaaagata attattttat aatagatgag aaaatagatt tagctcttga
     4861 aatagctaaa agagagcgat tatttatgta tcctatagat aaaaataaaa tttctcttta
     4921 tgtaagtatt ccattttgtc caactagatg tgtatattgt tcatttcctt caaattcatt
     4981 aaaacaattt ggacatttaa aaagagaata tgtttataaa ttgattgaag aaattaaggg
     5041 gtttgccaaa gttattaagg atactaagaa agaagttgaa actctatata ttggtggtgg
     5101 aactccaact acacttgatg aggaagaatt ggatttgctt ataaattcat tgttcaatga
     5161 attagattta agtaaaatta aagaatttac tgtagaagca ggtagacctg acactattac
     5221 agaacaaaaa ctaagagtgt taaaaaaaca caatgttagt agaattagta taaatcctca
     5281 aactatgaat gatgatacct tggtaaagat tggaagagag catagagtat ctgatttagt
     5341 agattgcttt aatatggctc gtagaatggg atttgataat ataaatatgg atataatttt
     5401 aggtcttata gatgaagatt taaatatggt aaagaataca ttggaggaga taaaaaaact
     5461 ttctcctgaa agccttacag ttcatacatt agccataaag agagcatcca acttaaatat
     5521 aaatatggat aggtataaag aacatctaac acaatatgaa gaaatggtaa aaatgataga
     5581 tttatcaatg aaatatgcaa aagatatggg attaaatcca tattatatgt acagacaaaa
     5641 acatatgttg ggaaatttag aaaatatagg ttatgctaaa gaaggatatg agtgcattta
     5701 taatatacag attatggaag aaaaacaaag taactatgca ttaggtgcag gtgctatatc
     5761 gaaatttgtc tatgtagatg aagatagaat tgaaagagta gaaaatgtaa aaaatgttga
     5821 acaatacatt gaaagagtag atgaaatgat aaaaaggaag aaagaagagg tatataaaaa
     5881 tgttgactaa agctcctaga ggaactaaag acataactcc aaaagaggct tacaaatggc
     5941 gttatgtaga aaataaattt agagaaatat gtgctttata tggatatgaa gaaatggtaa
     6001 cacctatatt tgaacataca gaacttttca agagaagtgt tggagatact acagatatag
     6061 ttcaaaaaga aatgtattct tttaaagata aaggtgatag agaaattacc ctaaagccag
     6121 aaggtacagc aggtgtagta agagcattta tagaaaataa attatatgca gatactcaac
     6181 caacaaaatt attttatgtg acaccttgtt ttagatatga aagaccacaa gctggtagac
     6241 aaagacaatt tcaccaattt ggaatagaag cacttggaag tgatactcct tcaatggatg
     6301 ctgaaataat cgcactagct gttcaatttt ttaatgaagt tggattaaat gatttggtag
     6361 tgagtataaa ttcagttggt tgtccagttt gtagaaaaga gtacaatgct ttattaaaag
     6421 agtatcttga ctcaaaagct gatatattgt gtgatacttg caatgagaga agagaaaaaa
     6481 atccaatgag ggttatagac tgtaaaaatc ctacatgtaa agaaaatatc aaagatatac
     6541 catttattgc agaccatttg tgtgatgatt gtaaatctca ctttgataaa cttcaagaat
     6601 acttaaagga aatgaatata aactttgtaa ttgataaaac aatagttaga ggcttagatt
     6661 actatagaaa aactgctttt gaaattatat ctaatgatat aggagctcaa agtactgtat
     6721 gtggtggagg aagatatgat ggtcttgttg aacaattagg aggaccaaaa ggtataagtg
     6781 gaattgggtt tggactagga attgaaagac ttttacttac acttgaaggt aatggaattg
     6841 aaatagaaaa ccctcaatct acggatatat ttatagtaac tataggtgaa gaagcaaata
     6901 caagaagctt taaattgtta aaagacttga gacaaaatca tataagtgca gataaagacc
     6961 atatagagag aagtgttaaa gcacaattta aatattcaga taaaataaat tcaaaattta
     7021 caatagtaat tggagatgat gagcttaaaa atgacactgc gacattaaaa aatatgaaaa
     7081 catcagagca aactactgta aaattaagta cattagttga agaattaaag caaaagctgt
     7141 aaaataagaa agtatgatgt tttgtgtatt agataaatat atagcattaa aattaaataa
     7201 aatggaggta gtatcttgga aactttaaag ggtttaaaga gaactcacta ctgtggagag
     7261 ttgagagaaa aaaacataaa tgaagaagtc gtacttatgg gatgggtaca gaaaaaaaga
     7321 aatttaggtg gtcttgtatt tgttgattta agagatacaa gtggattatg tcaaatagtt
     7381 tttgatacag atgtagataa agaagctttt gaaaaagctg aaaaattagg ggctgaattt
     7441 gtaatagctg taaaaggaaa agtatgcgaa agacaatcta aaaaccctaa tatgccaact
     7501 ggtgatatag aaatatttgc aactgaactt agacttttaa acaagtcaga aactccgcct
     7561 atttatataa aagatgatga tgatgtttct gaagcactaa gattaaaata tagatactta
     7621 gatttaagaa aaccatctat gcaaagaaat ttaaaattaa gacataaagt aatgaatatt
     7681 acaagaaatt acttatcaaa caatagattt tgcgaaatag aaacaccatt tttaatagct
     7741 ccaacaccag aaggagcaag agattatcta gtaccaagta gagttaatcc aggaaaattt
     7801 tacgcattac cacaatcacc acaattatat aaacaattat tgatggtaag tggtatggat
     7861 agatatttcc aaatagttaa atgctttaga gatgaagact taagagcaga tagacaacca
     7921 gagtttacac aaatagactg tgaaatgtct tttgttgagc aagaagatgt tatgagtatg
     7981 attgaaggtt tgcttgaagc gatatttaaa gaagttttag atgtagaatt ggcacttcca
     8041 cttcctaaga tgacttatgc agaagctatg tctaaatatg gttcagataa accagatact
     8101 agatttggat atgagttaac agatatatct gatgttgttt gtaactgtgg atttaaagtg
     8161 tttgctgatg ctacacaacc aggaaaaagt gttagaggta taaacgtaaa aggcaaagct
     8221 gacgacttta ctagaaaaca aatatcatca ctagaagaac atgctaagac atatagagca
     8281 aaaggtcttg cttggatgaa agtaggacaa gaaggagtaa cttctccaat agcaaaattc
     8341 ttcaatgaag aagaaatgaa cgctatactt acaagaatga atgctgaagt aggagattta
     8401 cttttatttg tagctgataa aaactcaata gtatttgatg ctttaggaca agttagactt
     8461 gaagtagcaa ataggcttaa tctattggat aaaaatgtat ataacttact atgggtaact
     8521 gagttcccag tttttgaaga agatgaagaa acaggcacat tctcagcaat gcatcaccca
     8581 tttacatcac ctatggatga agatttagac aagttagaag aaggtgataa gtcatcttta
     8641 agagcgaaag cttatgatat tgtattgaat ggttatgaaa taggtggagg aagtgttaga
     8701 atatcaaatt ctgatgttca atcaagaatg tttaaagcac ttggttttac agaggaaaga
     8761 gctaatgaaa agtttggtta tttattagaa gcatttaaat atggtactcc tcctcatgct
     8821 ggtctagctt ttggtcttga tagacttgtt atgttacttg caggaactga taatataaga
     8881 gaagttattg cattccctaa aaatcaaaat gctgtttgcc ctatgacaaa tgctcctaca
     8941 ttggcggaag atgagcaatt agaagaatta agtataaaag ttgatataaa agataatgaa
     9001 taatatgtag ttgcaagaaa ttaaaatgta aaaagtctgg atataaagta tatttatatt
     9061 cagacttttt ttattagaat tattttatat tttttctcta tatattttca taaattatac
     9121 ttgtattctc aaccaatata attaaaatac tatgtttaaa aattttacat aaacttggtt
     9181 gttttagaaa ctattttttt aatgagtaca cgattttaat taaagaatat ataacaaatt
     9241 tgaaataagt ctaatatatg acttaataga ttaaattaaa ataataaatt tggacgattt
     9301 atctatttat aacttaaatt atatcaaatt gataacaaac tagatattat acttttttgt
     9361 agaatagaat tacaattata taaagaaaaa ttctattaat agtgaaaaaa aagtagaaaa
     9421 aagtagaaat tattctttta taaagaaagg gagggataat attgcaagag agagaagaat
     9481 tagttttaaa tccagttcca aaagaggaaa gagttggatg gttagctcca ttgtttaata
     9541 tgcttggttc taacatagct atatctgaac taatggttgg gggaacttta attttaggaa
     9601 tgactctttc taatatgatt attacatcaa ttatagggaa cttaatatta gttgcaatta
     9661 ttatgataca aggttatata ggttacaaag aagggttaaa tacgtatgta ttagctaagg
     9721 gggcatttgg agagatagga ggaaagtatc ttatatctct attacttggt ataactagtt
     9781 ttggatggtt tggtgtacaa gcaggggttg caggactatc agtacaaaag atatttccaa
     9841 gtgtaaatct aacattagtt acagttatac ttggattatt tatggttgtt tttgcactgt
     9901 atggctttaa agctatggct aaatttaatt acttagttat accaccactt atgatattaa
     9961 tggtgtgggg agtttttaaa gctttttcta cttatggagt tgaagctatt tataattata
    10021 ctcctcaaac aactatgagt atggttgaag gtcttaatat tgtagtaggt cttgttatag
    10081 ttggtgcaat tatatcacct gaccagttaa gatatacgag acgagtaaaa gatatttgga
    10141 ttatcagttt tttaggtcta gggattattt cattattcca acaagttgca gctggtgtaa
    10201 tgtctatggg agcacctact tgggatataa cagaagtatt ggctaattta ggttttggat
    10261 gggtagcgtt tgttatttta atacttgcat cttggtctac aaacgtatca aatgcttatt
    10321 ctggtggatt agcattaaaa actatttttc caaatgtaaa aagaaatgtt ttgactttag
    10381 ttgcaggtct aataggtaca ataatagcag ctactggtat aatatttaaa tttcagtcat
    10441 ttttatcttt cttgggaatc gcagttcctg caatagctgg aattatgtgg tgtgagtatt
    10501 attttataca aggaagaact tataagcata gagaaggaat aaattggatt gcagtaataa
    10561 gctggttaat aggttttgca gcgtcgtatt atagttcaaa aataaatttc ttaatacctc
    10621 cgataaatgg aattgtagtg agtatggtta tttattatat attaatgaaa tgttttggaa
    10681 tcaaagataa ataaaggggg caattacatg ggtcgtaagg tgaaaatagg tataattcaa
    10741 caacacagtg tacttggaga tgttaaaaaa aatatagaaa aggcagtaga gatgatagat
    10801 gacttaggaa aacaaggggc agatataatt tgtttaccag aactatttgc tacaggatat
    10861 aatttagagt ctttaggtgg agttaaaaca ttagaattaa taagagaaca taataagtac
    10921 atagaggaat caatgtcaga agctgctaaa agaaataatg tatacctgat atctccatat
    10981 ggaaccttag aaaaagggtc tacacatgtt tataattcag cagttatatt tgatagaaaa
    11041 ggtaagatta tgggagaata ctgtaaaaat catctgtggt cattagaagc agtatacttt
    11101 aaaggagggg aaaaagtaga ggtatatgat gctgattttg gtagatttgg tgtaatgata
    11161 tgttatgatg caggttttcc agaagtatcc agagaattga cattaaaagg ttctgaaata
    11221 atatttatac catctgcatg gagaatacaa gatgaagata tgtgggattt aaatgtatct
    11281 caaagagctt tagagaacac tgtatataca gtaggtgtaa atttagtaag taatgactct
    11341 aatttaatac tatttggaaa gagtaaaata tgcaatccta gaggtactgt aataactcaa
    11401 ttagatacat acagggaaaa atatgtatta tcagagattg atttagatga aataaaaaag
    11461 tatagggaag atatccccta tttgaaagac agaaaaagta gctataacat ataattctat
    11521 actataatac aagttcaata taaaatgata aaaagaggat gtatcaatat taagattaaa
    11581 tttattatac tttgatatat cctctttaat attatcaact ttgatttaaa tatttttaaa
    11641 atagttgata agaaagtttt tgaacaacaa cacagcttta gattcatagt tttcgctaaa
    11701 ccatgaaaga tttaaatatc ttttaaattc aatatctttg atatgtaaca acttaatctc
    11761 agggtcttga gataatcccc atgattttcc acatataaat ccaactcctt gaagtgattt
    11821 tataagagca taaattgtat atggtgaatc agactcaaat gctattttgg gtttgaaatt
    11881 agctgattcg cacaggatat caattacctc tctataattc tcacccttcg ttataactat
    11941 aaagttttca tctgaaactt cgcttagata tacttcatcc tttaatgata aaggatggtt
    12001 tattgaaact cctaatagta tttcttcgca tgttaaagtt atgctatttt ctgagtttag
    12061 ctttgtaaat gaagaagata tgtacaaatc aaaatcactt tttttataag atgatggcaa
    12121 agtatgtgac acattaaaag taatattggg atatagtttt ctgaaattac tcaaaagtga
    12181 tggtaacaca ttagccgctg caggagataa tatctttact tctcctgaat tttctaaaga
    12241 agtgtcttgt aactcctttt ttgaactttc tactaaatct aaaatgctat ctactctttt
    12301 tagaaacaat cttccatact catttaaaac aatatatctt ccttttctgt caaataactg
    12361 aactccaagt tctttttcta aggaagatat agttttgctt aaagctggct gtgctatatt
    12421 taacttgtgt gatgcttttg ttatgtgctc cattctagca acagtttgaa aatacttaag
    12481 atgcaataaa tccatagtat aaaatacacc tcttttaata tgacttgagt tatatcaatt
    12541 ttataatcaa ctccacatta tacattataa tataaagttt atataataaa ctacaatata
    12601 ttaaaaagtt atttaaattg ttatttatat tttgtacata atctaattaa aaaaagtaac
    12661 tttaaatgat taactaagga aatgttgtta caaactttag ttatataaaa aattcatttg
    12721 atgagcaaag tattaatgtc aagtaagaat aaaagcttat tagataagaa aaataatatt
    12781 ttataataat gatgttactt aaattattat gttatactat tttaaatagg aaacatttag
    12841 atactataat tttaggggga gtcaagatga aattaactaa atcaatatca tcaatgctgt
    12901 gcttatctct agttttaagc ttatcaccag tagcatatgc aaatgaaata agtagaacaa
    12961 cagttgagaa tactttagta ggtcaggata gatatcaaac tgctgttgaa gtaagtaaaa
    13021 aaggatggag tagtgcaaat gaggctgtag ttgtaaatag tgaatctatt gtagatgcat
    13081 tagctgtaac accatttgct aaattaaaaa atgctcctat attattgaca gaaaaaaata
    13141 atctaaattc acaaactgag aaagaattaa aaagattagg agttaaaaaa gtttatataa
    13201 ttggtctatc tagttctgta tcaaatgatg tgcaaaaaca gttagaatct aataatttaa
    13261 ctactgatag agttggtggt agtgatagat accaaacagc tttgagtata gctaaaaaaa
    13321 tagaaggatt aaaagatata tcagaaatag ctgtagtaaa tgggtacaca ggtcttgcag
    13381 atgcagttag tatagcttct gtagctgcta ctaatggaat ggttatactt ccagtatctg
    13441 atgcatctgg tatatctagc tttaaagatt ttatagaatc taagaatata aataaatcat
    13501 acataatagg ctctactaat gctgtatcag ataaaataaa acaaagttta cctaattcag
    13561 aaagaatagg tggagcagat agaaatgaaa ctaatggtaa agtaatagaa aagttttata
    13621 catctaataa attaaacaat gtatttgtgg ctaaggatgg aatgaaaaat caaaatcaac
    13681 ttatagacgc tttagcagta ggagtactag catcaaaaga aaattctcct gtactaatcg
    13741 taggagaaca attaagtgca acacaaaaga gtgtattttc gaataaaaat gctacctcta
    13801 taactcaagt tggtagtgga ggaaatgaaa atgcatttaa agaattaaaa gatttacagg
    13861 caaatagtaa caataagaga gtaatgtatg taactaatac agataaggta aatataagaa
    13921 atgatgctac aatagaagct tctgttatag gatatctaaa taatggtgat gaagtagaag
    13981 ttttagatgt gttaaaaact ggatgggtag aaataaaata caatgaaggt attggatatg
    14041 ttagtgggtc ttatttgaca aataataagc ctgataatag taatgaaaat ataaaaataa
    14101 agtatgtaaa agaaaaagat ggtctaaatg ttaggaaagg accaagtaca gaagatgaaa
    14161 aaataggtca cctttcatat ggaagcaaag tagaaacaat agaaatgttt gctactggat
    14221 gggtaaaaat aaagtataat ggtggatatg gttatgtaag taatgattac ttatctgata
    14281 caccagttat ttaattataa gtaacaataa aaaaactgct ttaatttgta aagcagtttt
    14341 tttataaaaa tttaagtcta ataactattt ctattttgat accttaatcc tgggatttat
    14401 tattatggat attatcaaca tacaaacact ccaaatccag tgtctaattc caaacttaga
    14461 tattaataaa ttcagtcctg ctatggtaaa taagcagtat ttaattacaa ttaaaagtgt
    14521 atttatagaa agccccttca tagaaagtat cctaaatcca ataatagaca ttctaagtcc
    14581 tataaatgcc aataatatac ctgcaatatc ctgaattaag taatagtatt caaatttcat
    14641 attcaatcaa ctcttctctg agcagtatca aatatttcta actcttcaat ttcatcatct
    14701 gtgtatgtaa gcatagtaaa accagttaca ccataaataa atgaaattat aggtgtaatc
    14761 caacttaaga aacaatatgg aataaattct agtgggctta caccaagagt ctgagcacag
    14821 aaaacagcat ttgaattcca tggtattaat ggaccaccaa gtgtagctgt atcttctata
    14881 attctagata gattttgtgg ttttaattta aattctttgt atattggaga cattagagtt
    14941 ccgacaaata cagaagtgaa catcattgat gaactaaatg catttgcaaa gtaacttact
    15001 ataaaagtag acccaaccaa tttagttctt gattttccta ttttagttat aagaggttga
    15061 agcagtactt ctaagaatcc agttttttgg agaataccag caataacgaa aactacaaat
    15121 actagtaaaa cggtttcagc catactcatt attccaccac gatttaatag tttgtcggca
    15181 tagacaaatc cagtatctat agtaaatcct gatagcatgt aatttaaaac atcacctatt
    15241 ttttctcctt cttgaaatac tgctataata agtcccatga tagctgaact tagtatagat
    15301 ataatgggtg gcttttgtag caataaaagt aataataaaa atattattgg aatcattgct
    15361 actaaaccaa tatgaaagtt tgaatttaat acatccttga tttgatttat ttgaacatag
    15421 tctatagtat tatttgaata cttaaatcca attactgtat acagaactat acatattacg
    15481 tatgcaggtc ctgtggtata aaacatatgc ttcatatgtg taattacatc tgtcttacaa
    15541 actgctgcag ctaagtttgt agaatctgat agaggtgaca tcttatctcc aaagaaagca
    15601 ccagaaatga ctgcaccagc tgttaaacca agaggaatac ccatactagt accaatactc
    15661 atcattgcta taccagaagt tccaagagtc ccccaagatg taccagtagc aacagaagtt
    15721 aatgaacata cgattaatgt tgtaagcaga aaatatttag gggttattat tgttagtcca
    15781 ctatatacta ctgttggtat tgtaccagca gctatccaag ctgctattag tacaccaaca
    15841 gaaagtataa ttaagtttga ttgaaaggca tctttaccaa cttcaaatcc aaaagcttct
    15901 atttcatcat tagtataacc aagtttcata gctgcaggaa ctactataag ccatgaaagt
    15961 aaaaacattg tagttataga agcattaaat acaactatac caactgatgt tagagctatt
    16021 attgcaagca ttactaacaa ggaatatcta aatgaaggta tttttctttc tgtattcatt
    16081 ttcatagtgc aaattctcct taaatatttt tagttaattg cattttttat tcttataaga
    16141 gcttcttcaa gcatacttct tggacaagct aagtttattc tttgataccc acttcctcca
    16201 atgccaaagc tgtttccttg atttagagca actttacctt tttgtacaag tatactttct
    16261 agttcctcat cacttaatcc aagtgcacta aagtctaccc ataataaata agtaccttct
    16321 ggttttctaa ctttaagttt tggcatattt tcatttatat attttatagc aaaatctata
    16381 ttagattcta aatattctaa aaatgattct agccaagatt caccattatt atatgaagct
    16441 tcagttgcaa ctaagctaaa gcaattattt ctctttatat caattctggt aaaggcatca
    16501 tctaacaatt tatagtcttt ttcatcaggt agtactacat atgatgattg aagaccagct
    16561 atattaaaag ttttagttgg agccatacaa gtaattgtat ttttttcaaa ttctttagaa
    16621 atagatgcca taggaatatg cttatgtttt tttaatataa tgtcactatg tatttcatca
    16681 gatatgattt ttacattatg cttaagacaa atatctccta gtttttttaa ttcatctttt
    16741 gtccatactc taccaactgg attatgagga ttacaaagta tgaataattt tacatcttta
    16801 atcttgtttt caatgtcttc ataatccatt atgtagtttc cattttctag tttttgtaat
    16861 gggcttataa ttagttctct attgttattt tttacaacac tattaaaagg gctgtaaact
    16921 ggttcttgta tcattatttt atcatttgct ttcgtgagtt catttattaa aagacttata
    16981 gctgggataa cacctggact ataaattaac cactcacttt ttattttcca gttatgtctc
    17041 ctatctagcc agtttactat agattcattg taagaatctg gtcttgttgt gtagccataa
    17101 atctcttgtt ctagtctatt ttttaaggaa tcaattatac aaggtgcagc cttaaaatcc
    17161 atgtcagcca cccacatagg aagtaaatca ttagttccat actttttttc catttctgac
    17221 catttagatg aaaaattatt gcttctatca acaatctcat taaaattata attcattgta
    17281 aatacctcca gtaattttaa ttttatgtta ataatattaa gcaaacaacg tgccaatatt
    17341 gataataaaa aatatttatt cacaagttaa tatttaatat taagtaattt cagagcatag
    17401 ataagataaa aatattaatt atacttaaaa ctttgtggaa aaggttattt ctaataaatt
    17461 tttaaatata tgaaaatgta attaaatagg ttaaataggt ttaactgttt aacttgttag
    17521 acctatttat attttttaga aattctactc cttttgatgt tataattgaa cctccacgac
    17581 caacaagtat gtctacaagc ccaaagtttt gtagttctaa aagcatattt ctaatctgtt
    17641 gttctgtcaa aaatctattt tcctctaaag ctattttaca taagcttttt ctacctattc
    17701 tttcttttaa atcataagcc ttttttaaat tatccaatat aaatatgtat tcatctagtg
    17761 accttttaaa attatagtcg tatttcatga tgtttttgtc tttgccaatg ttgaattgat
    17821 acttttttat attatcactt actttattac aaacggttct actataattt gaatctatag
    17881 tatccaatat atattcaggt aaatcacata tttctataat atctttttcc aagtaacaga
    17941 agtattctcc taagtttctt aattctctta catttccttc ccatctatac attttaaaga
    18001 tttcttttac ttcctcactt agaataaatt tacatggaat atcatatttt aatgaaccaa
    18061 aaatttcaaa tatatcagta ggtctttccc taagtggatg tattttaagg ggaagaacat
    18121 tcaacctaaa atatagatct cttctaaaca tatttttaga tacaagttct tttaaatttc
    18181 tattagtagc tgcaattatt cttacatcaa tgtcaatgac attattagaa ccaatacgtc
    18241 gtacttgttt ttcttgtatg actctaagta atttagattg agaatttaaa tccatttctc
    18301 caatttcgtc taaaaatata gttccattgt tggctaattc aaataaacca attttcccac
    18361 cttttttagc accagtaaaa gccccttcat catagccaaa taattcactt tgcagtagat
    18421 tttcttgaaa agtactacaa tttactgcga caaaaggacc atcttttctc ctagaagcac
    18481 tatgaatgga ttgagcaaat aattctttac cagtaccact ttcaccaatt ataaggatag
    18541 atgaatttga ttgagccatt tgattagcaa ttttttttgt attaatagtt tgtatactag
    18601 aacctaatat atcatcaaat gtgtatttac tgatattacc agagtttact agttgtgctc
    18661 ttaatttagc ttgctttttt tctaattgag aaaattttgt catctttagt ataaatccat
    18721 caaatacatt taattgtata tgtttaatct ctaaatttat atcaatatga tttattttaa
    18781 tcaatttctc aaaaaataaa gttttattgg tcaatatgct ttggaaattg atgtctttta
    18841 tgcaatcacc aacaaatgag tcaatcattt catttgcatt tatagacaaa atttttctag
    18901 ccatatgatt atagaattga attattccat cgttatttgt gcatataatt ccatcatcaa
    18961 ttatagatag taacaaatca aattgtcttt caaacttatt tgcatctaac aataactcac
    19021 ctgtactata acttgtagga attatttttt ctacgtattt ttttattagg tcatctttta
    19081 ttagatgttt taatttggtt tttgtagcaa tttcaacaat acaacttaaa tctattattc
    19141 tgtattttaa atctataact tcacaatttt taaacttagg caagattttt tcgccaggag
    19201 ttaggattac agcatctgtg ggagtaaact caatctctgg atagcagggc aggatattta
    19261 tattgtctat tcctaatcta tgtattaaag aaattgtttc tattgccata tctttactta
    19321 agttatatac ataaataatt ttatcttttt ctaatttgga tatcatatca atactgttct
    19381 tttcaaatgt caacttggga acaatgattt gagcatcata gtgagataat tttactattt
    19441 catcatattt taaataagca gataggagta taagtttttc tttaaaaaac tttaaatttc
    19501 cctcttcaaa tgagtataaa tttatcttta gattatcacc taagaattgc cttatttgat
    19561 tttcataaat ttctccagcg tcttttttta aacttacaat agctaattcc atttgtttca
    19621 ttttaacctc tcatacaaat ttgacttatt ttagattaca taataattaa attagattaa
    19681 ataatattat ttaattatta ttataaattt attatagact acacattgaa gtaaaagaag
    19741 tagctaaaat tattcctttt atttttgttt tttgtatcta tttggtataa tagtattaga
    19801 gcgtataata atttaggcaa aattttttaa ggaagactag aggagaatat aaaatgaaaa
    19861 aatgtcttta ctgtaatagg gatataaagt caccaaataa taaattagct ataaaatatg
    19921 atgacaatat gaatatatat ccatgtcgtg cagaatgtaa ggaaaaaatt gaagaatata
    19981 tagcaaaaaa acctacatat aagtttataa atatattaga ggttctttta ggtgtgggcg
    20041 gaatattctg ctttttgagt aaatggacta ttgcagccca agttgtactt ggaattgatg
    20101 cactggttat atttttattc ccattggcag gatttaaaat gaatggaaag ttaagtatgg
    20161 aacaaactaa gaatcaaagt aggtctattg caatatcaat tttggcattt atagtaataa
    20221 taactgtatt atattttaaa caaattggta agtagagcat aattgtataa gacatcatta
    20281 gagtatgagg tgagtttatg gaagaaagaa aattagcacc aaacaatgag agggaagctc
    20341 tcataaaaag attaaaaaga atagaaggtc aagttaaggg tatacaaaag atggtggaac
    20401 aagaacgtta ttgtgttgat atattggttc aaatatctgc tataaggtct gctataaata
    20461 gagtaggcac aattatctta gagaatcata taaaaggatg tgtagcagaa agcattaaac
    20521 atgatgatgc tgataaaact gaagagatga taaaagagct tatggatact ataaataaat
    20581 ttacaaaata aggaggcggt tagttatgaa tcaacaagga tttattatgg aaattgttga
    20641 tgatagaact gcaaagttaa aaatgcaaag acattcagct tgtgctgctt gtggaaaatg
    20701 tgctactaca aattcagcag agtcaaaaga aatattagta gaagtagaca acacaattgg
    20761 tgcaaaagtt ggtgaccatg tagaagtgag tatggataat atgaatgtat taaaagcagc
    20821 tgtaatggct tatataatac ctcttatagc acttcttgta ggaacaatag ggacttatta
    20881 tatattgggg gcaattggaa tgacaactgg agttgaagcc ataagtggat tagttggaat
    20941 ttgtcttaca ataatctgtt atttatactt aaagaagaat gacaaaaaat ttagagacag
    21001 tagagaattt ataccagtaa taacaagaat attaattgac ctttaagata attataatta
    21061 aaaaatagca atgtgctttt taaataaata ctcaatacgt atttgtagag tttaagtaca
    21121 ttgctatttt tatattttta ttttgatttt ctgtaaggaa taaagttttt tctgattttc
    21181 aaaagattca atgttcttac aaaagaagtt acaactaata caccaagtgc tattgcagca
    21241 cttagaaata gcgcatctgt accttttgac atagccaaag aataattttt ctgaatcaaa
    21301 gacaacattg tattatatag cccaattcct ggtattagag gaagaatacc tggaattacg
    21361 aacacaatag ctggttgctt tagttttctt gctaaagtct cactgcacgc tgagaccaaa
    21421 gcagcagcga taaaacctgc aaaaactgca tttgtagtaa aattaaataa ataaacatat
    21481 acaatccaac caattccgcc agttactcca gctggaagaa gtagatatag tggagcatta
    21541 aagaaaacag caaatccagc agttgcaaca cctgaaaata aaaaatgcaa aatcatagac
    21601 atatcagtca ttagaaagga cctcctattc tataccatgt atcaaggaca aaaccaactc
    21661 cagaagcaat tgcaataata atcatagcag catcaaaagc acgagaaatt cctgatatta
    21721 aattccctga tattaaatct ctaatagctt ttataaaagc aactcctggg agtagtggca
    21781 ttatagcgcc gactataagc attttagggt catctaaaat gcctatttca actagtagaa
    21841 ctcctgaaag tgcaattaaa aaagaagata ctaaacagga aaatgcagat attgcactta
    21901 tcttcatcgt tttatcataa gtaattgcag caaagattgc tattataaac gtaagtataa
    21961 aatttaatac ataattccct ccaagcaaac aagcaaaaca agcagatgca attcctgtac
    22021 aagagtaaaa taaccataaa ggataaggtt ttacatcttg gatttcataa agacgtgcaa
    22081 tggcattatc aatatcatac tctttgtcac taacaaattc tcgagaaaaa ctgtttaata
    22141 aagaaatctt atttaagttt atacctcttg attgaatcgt tttcataaac gaaaagccat
    22201 caaatttctc atctgaaata attattactg taggagttgt aaaaatattt acatgataaa
    22261 accctcttga cctgcatatt ctaaggacac tgtcttcaac cctagatgtt tcagctccat
    22321 tttttatcat cagttcccct acaaatagag ctagttttag aatatttttc ttatattcaa
    22381 gttcctcatt ttgattcata aatacacctc caaattaaaa aacgtaatat tgaaattata
    22441 tcaaattttt aagtaatatt ccttaatatc tatacaaata tattatcttt tttttgtttc
    22501 aaaatagtca catttaaatt atatccaatt aaaagaatta gacaatatta tttattatat
    22561 tttaacttaa aaataaagaa agtaactatg ttaaatatgg aaaaatggca aaacacttga
    22621 ctattaattg gtaaaggact ataataataa attatcatat tgataaaaac gaaaattatg
    22681 aaatgtctgt aaaaataaaa tttaaataat aactaggagg agtaatatgt ttgagaactt
    22741 gaaaaaagcg gaccctgaga tttatgaaag tatgaaaaga gagttaaaaa gacaacaaag
    22801 aaatattgaa ttaatagcat cagaaaacat agtatcagtt ccagtaatgg aaactatggg
    22861 aagtcatctt acaaataagt atgcagaagg ttactcaggc aaaagatatt atggtggatg
    22921 tgaatacata gatgaagttg aaactcttgc aatagataga atcaaaaaaa tatttggagc
    22981 agaacatgct aatgttcaac ctcactctgg agcaaatgca aatattggtg tatattttgc
    23041 tatgttaaaa ccaggtgatg ttgttatggg aatgaattta tctcaaggtg gacatttaac
    23101 tcatggtgct ccagttaata tttctggtca atattataaa ttttatgaat atggtataga
    23161 taaagacagt ggacttatag attttgatga agttagaaag atagcacatg aagtaaaacc
    23221 taaaatgata gttgcaggag caagtgcata tcctagagaa atagatttta aaaagtttag
    23281 agagatagct gatgaggttg gagcattgct tatggtagat atggctcata tagcaggtct
    23341 tgtggctgct ggtcttcacc aaaatccatg tgaagtggct gattttgtta caacaacaac
    23401 tcacaaaact ttaagaggac ctcgtggggg tgttattctt tgtaaagaaa aatatgctaa
    23461 agacatagac aaagcaatat tccctggtat tcaaggtgga cctcttgaac atataattgc
    23521 ttctaaggca gtttgcttta aagaagcttt aagtgatgag tttaaagaat atcaggtaca
    23581 agttgctaaa aatgcaaaag ccttagctga agaacttatt aaaagagatt ttaaattgat
    23641 ttctggaggt acagataatc atttaatact attagatttg actaataaaa atataactgg
    23701 taaagcagca gaaaagagat tggatgatgc ttatataaca gctaataaaa atacaattcc
    23761 atttgaccca aatggtgctc ttgttacaag tggaataaga cttggtactc cagctgtaac
    23821 tactagaggt atgaaagaag aagatatggc aataatcgct gaggctatag acctatgttt
    23881 aacttatgat gaagaatcta aggctagaac attggttgtt ggtttaactg aaaaatatcc
    23941 tttatatgaa gaatataata ttatggatta attataaaga acaactaaat aaaatacatt
    24001 taaataatta caatgattgt gtatgtattt gaatatattt agatat
//
LOCUS       DAELJO010000040        24017 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELJO010000040 DAELJO010000000
VERSION     DAELJO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.040"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      130..231
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            304..1647
                     /locus_tag="KPB88_001408"
     CDS             304..1647
                     /locus_tag="KPB88_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0053700.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1826..3190
                     /locus_tag="KPB88_001409"
     CDS             1826..3190
                     /locus_tag="KPB88_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053701.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3368..5233)
                     /locus_tag="KPB88_001410"
     CDS             complement(3368..5233)
                     /locus_tag="KPB88_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0053702.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVANYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5469..6686)
                     /locus_tag="KPB88_001411"
     CDS             complement(5469..6686)
                     /locus_tag="KPB88_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0053703.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6886..7749)
                     /gene="aroE"
                     /locus_tag="KPB88_001412"
     CDS             complement(6886..7749)
                     /gene="aroE"
                     /locus_tag="KPB88_001412"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0053704.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7831..9753)
                     /locus_tag="KPB88_001413"
     CDS             complement(7831..9753)
                     /locus_tag="KPB88_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0053705.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCVACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9832..10680)
                     /locus_tag="KPB88_001414"
     CDS             complement(9832..10680)
                     /locus_tag="KPB88_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG0053706.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11093..11974
                     /locus_tag="KPB88_001415"
     CDS             11093..11974
                     /locus_tag="KPB88_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0053707.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12182..13045)
                     /locus_tag="KPB88_001416"
     CDS             complement(12182..13045)
                     /locus_tag="KPB88_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG0053708.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13200..15134)
                     /locus_tag="KPB88_001417"
     CDS             complement(13200..15134)
                     /locus_tag="KPB88_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG0053709.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15246..16259)
                     /gene="galE"
                     /locus_tag="KPB88_001418"
     CDS             complement(15246..16259)
                     /gene="galE"
                     /locus_tag="KPB88_001418"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG0053710.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16283..17152)
                     /gene="galU"
                     /locus_tag="KPB88_001419"
     CDS             complement(16283..17152)
                     /gene="galU"
                     /locus_tag="KPB88_001419"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0053711.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17350..17670)
                     /locus_tag="KPB88_001420"
     CDS             complement(17350..17670)
                     /locus_tag="KPB88_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053712.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17660..19651)
                     /locus_tag="KPB88_001421"
     CDS             complement(17660..19651)
                     /locus_tag="KPB88_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053713.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19774..22317)
                     /locus_tag="KPB88_001422"
     CDS             complement(19774..22317)
                     /locus_tag="KPB88_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG0053714.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGVDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22595..22929)
                     /gene="rnpB"
                     /locus_tag="KPB88_001423"
     ncRNA           complement(22595..22929)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPB88_001423"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23061..23738
                     /gene="srtB"
                     /locus_tag="KPB88_001424"
     CDS             23061..23738
                     /gene="srtB"
                     /locus_tag="KPB88_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0053715.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23898..23972)
                     /locus_tag="KPB88_001425"
     tRNA            complement(23898..23972)
                     /locus_tag="KPB88_001425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23938..23940),aa:Thr,seq:tgt)
BASE COUNT         7672 a         4153 c         2708 g         9484 t
ORIGIN      
        1 acatattcaa ttattgaaaa caataatttt tggaaacaaa taaccttaat tttttataaa
       61 agcgacaaaa aaacgaataa ttttattata tttattgtaa ataatacaat attacgatat
      121 aatacaacag aaaaacaaat agaaaatgtt catataatat tagtaatacg gtctaatagt
      181 ttctacaaac tcaccttaaa tgagttttct atgaatagaa tgtcaacttt gaatatatgt
      241 tctattctat ctatatgaat atatattttt ttgtttatat cttaaagttt tttggaggta
      301 aaaatggtac caaacactaa caatgtacaa ccaggtggaa ataattcatc attaagcttt
      361 ttagacaaaa tgtttaaatt tagcgaacgt ggctctagcc ttaaaacaga ggtaataggt
      421 ggagttacta catttttaac aatggcatac atagttttcg taaatccagc aatattatct
      481 gaagctggaa tggataaagc cgcccttata acagttacca tactagctac agccattggt
      541 acacttatat ttgcgatttt aggaaatgca ccatttgctc tagctcctgg tatgggatta
      601 aatgcattct tcacatactc tttggttata ggtgcaggaa tcccatggca aacaggtctt
      661 ggggtagttt ttctatctgg atgtgtcttc tttacactcg cagtaacagg tcttcgaaag
      721 acaattgcag acgcaattcc tagagaattg gcagtagcat cttcagcagg tataggtctt
      781 ttccttgctt ttgttggttt aaaaaatatg ggtgttataa tagctaatcc agatactata
      841 gtttcacttg cagattttac accaacagtt ttaatagctc tttttgctct tatcttaatg
      901 gcaatactag aatataaaaa agttaaaggt ggaatattga taagtatagt agttgcaaca
      961 ataatatctg ttatatttgg atttgtggag ttgccaacag caataatctc aacacctcct
     1021 agtatagcac cagttgcttt taaattagat attgtaggtg cattgaaatt ctcattaatt
     1081 ggacctatat tctctttcat gtttatagac atgtttgatt cattagcatt tcttattact
     1141 tgttgtaaac aaatgggtct tgaagatgag aatggtgaaa taaaagattt aggaagaatg
     1201 ttatatgcag atgtttcttc tactttaata ggctcattct taggaactag tactgttaca
     1261 acatttggag aatctgcttc tggtattgca gcaggtgcaa gaacaggact tgcttcaata
     1321 gtaacagcag cattattctt agctacactt atatttacac cattacttgg tatagttcct
     1381 acttacgctg cagctcctgc acttgtaatg gttggtgtat tcatgtttga aagcataaaa
     1441 cagattgatt ttgctgatag caaaatagct gtacctgctt ttgttacagt tttattgatg
     1501 cctttaactt atagtataag tattggttta tgctttggat ttatttcata tataataatg
     1561 catgtagtag ctaaagaaac tgataaaatc agtattacac tttggattat aggtttactt
     1621 gcagttgtaa acttagttct aagttaatat ttttaaatag ttctaggtca atattttaaa
     1681 ttatatagaa taaaatctta tttattttaa atttataaat agcaaaatca ctttatatgt
     1741 aaattgattt tgctattaac tttgtataga aaatttatta tcataatata tagatataaa
     1801 aatatataga aaaggagtgg tgtttatgtc acaattactt ataaaaaatg catatctaat
     1861 tactatgaat gcagatagag aggtatatga aaatggaagc atacttattg aaaataatat
     1921 tataaaagag gttggtaagt ttgatattaa tattgtagat aaagatgcag agatatatga
     1981 tgctaaagga aaaatactaa tgccagggct agtcaatacg catgttcact tatctcaaca
     2041 attaggacgt ggtcttgcag atgatgttgt acttttaact tggcttagag agagaatttg
     2101 gccatacgaa agtagcttca attatgaaga ttccttgatt tcatctactg catgttgtgt
     2161 tgaattgata aaatctggtg tcactacatt tttagaatct ggtggtcaat atgtagatgc
     2221 tatggttgag gctgttgata aaactggact tagagcctgt ttagctaaat cagttatgga
     2281 tacaggagat ggtcttcctg aggcatggca aaaaactact gatgaggaaa tcaatactca
     2341 attagactta ttccataagt ttaataatac atctaatgag agaattaaaa tatggtttgc
     2401 attaagaact atttttaata caactgaaga attacttgtt agaactaaag aactagctga
     2461 taaatacaat acaggtatac atatgcatat tgctgaaata gctgatgaaa tttcatttgt
     2521 aaagcaaaac aatggagttg gaacagttga ataccttgat aaactaggtg tattaggacc
     2581 aaatctttta gctgctcata cagtttggct tactcataag gaaattgatt tatttagatt
     2641 acatgatgta aaagtttctc atgacccagg ttcagctatg aaagtggttc taggatttgc
     2701 atcaattcct gaaatgttag aaaaaggaat acctgtttca attggtactg atggagctcc
     2761 ttcaaataat agaatggacc ttatgaggga catgtatctt acatcactaa tacataaagg
     2821 aagaacttta aacccaactg tcgtaccagc tgaagaagtc ttagaaatgg ctactataaa
     2881 tggagcaaaa tgtgctttac tagaaaaaga aattggtagt cttgaagtag gaaaaaaagc
     2941 tgatatgatt attctaaatc cagatacaat tcattgttta ccaatgcata atccaattgg
     3001 aaatatagtt tattctatga ctagtgaaaa tgtagactct actatttgtg atggtaaatg
     3061 gttgatgaag gaaagaaaag tattagttgt agatgaatct gaattattaa ataaagttaa
     3121 aaaacaagct tctaaaataa aagataaagc agaagttaaa attccatcta aatttaaaat
     3181 tataaaataa cataaatttc aattgttttg taaatatgaa tatataaata tatttatata
     3241 catcatatat acaaaacaat tgttttttta ttttatattc attagcattg ctcataaaat
     3301 acaccataat tccagaaaaa ataaccaatc agttaagatt acatcatcta agcaatatat
     3361 aaacgtctta gtttataagt tttattctac tcttatctaa aaccatcttt aaagagtttt
     3421 caactgtttc aattacatct ttataactca agtacacatt tgcgtagata caatcttcat
     3481 ataaattata ttcagttact ctacaataca gcagttttcc tccatctaat aaattgctca
     3541 aaatatagtt ttcttttctt gggacataac ctatcacata tccagtttca ccaactattt
     3601 taatagcttt ttcatcatat agattatctt tctctctagt taaatagtat atcttacttt
     3661 tctccaattc tttttcaaga tattctctat ttctaaacct tgaaccagca acagctattg
     3721 aagtcaaata gatatcctta ccatgttcat ctacttttat tttttcaata tttacaaatt
     3781 ctttttttaa attattactt tcatatagta ctcttcttaa ttcatccttc aatataacat
     3841 ttttttcttt ttcatacgct tctttaacaa tttttaattc ttcaagagat agtttctctc
     3901 tgtatctttt taagatattt atggattctt ttcttacttc atttatattt ccaaatattc
     3961 cttttaaaga tatattcttt ccttcaggat atagtgattt acttccttta agcacaataa
     4021 aaaataacat atcatcataa tattcttgag tcaatttatc tttttctata tctttcattt
     4081 ttccaattaa atcatctatt ttaaacgtct cttcaaaaaa atttaataat tttaatctag
     4141 aacttgtcgt tcccttttta gatatatgcc aatatacctc aaaatctcta atatctctat
     4201 tcagatatgg caacaagtca tcaaaagata agttcacatc ataaatctct gacattttta
     4261 taatatcttc tgtttttcca ctagcatctc ttaatgcttc aaaatatata tttttccatt
     4321 tttcactgag caagatatca tttatttctt tttcaaattc aatcttattg cactctatat
     4381 tttcatcttt taagacgttt atagctatta ggaaaattga atataaactt tcaaagttag
     4441 tcccataatt tgctacagta ggtaaaaatc gattcactaa ttctaattta ttacctatat
     4501 atttaaattc tacacttagc aagtaattgc atataagacg tgctaaatta tttatctttt
     4561 ctttatctaa atctcgtttt tcaagatatt cattcaaatc tacaattgaa ataatatagt
     4621 tcatcaataa tcgttcgtac tttgtatcct tataaccatc ttcaattaga taggaattta
     4681 ttttagaatc taaattttcc atattaacaa tagcaaatac ttttattgaa cctgtagcct
     4741 ttttagataa attaaataaa tatgtattgt aaaattcaag ttttttaata gtattactca
     4801 gataaaatac gtattctcca gatttagaaa atgtatctac aacttctctt aagttttcta
     4861 catttagata tttctcactt aaaactaacc ctagttttac atactcaact tttttactct
     4921 tatatattaa ttctttagtt aatttatata accatttctc atcaataatc atttctttta
     4981 ccaaagtatt tatctttttc ataaaaattt cgaaataaca aattaaagac ttagaattac
     5041 tatcctccaa taaataaatt tcattagatt cacttttata catatttaaa atttcatcta
     5101 ctagattatt tgcaagctct acttgttctc ttaacggtat actttcatat atacataggt
     5161 aattcatttc aaatgattct ttgtatccat cataaccatt aaattcctgc aatcgcttaa
     5221 aaacattctc caccaaatta tcctccttta catgatgtaa tttatattga ttatactact
     5281 tatttcgtca agtttacatt tatacgcaaa aaaatattac tagaaattga aattctagta
     5341 atatccttat tattaattat gtaaataccc tatcttataa ctgtaaataa tatccttatt
     5401 aattttaatt acttttaata aaaatctatc ctagatttag acttctctat gcatatatta
     5461 tactaatatt agacatcata atcatacact ttttcttttt catatctcat gtttacaaac
     5521 aatgcaagta aaattccaac aaatgttatt gccacattaa acaatacaac atattttggt
     5581 ccattaactc cccctgattt tgtaataata cctgctatat ttaaaataac atagtttgct
     5641 atactagatg caatcataac tatagatgtt atttttcctt tattagttgg aaacatttca
     5701 tttgcagttg acgttgttaa ctgaagaaca cctcctgctg ctgaataacc tattacaaat
     5761 ccccctaaca tacaaatagt tggagtttga acaaaatata caactaatag cataagtaaa
     5821 gctattatag gatatataac taatattctt attggtttaa tatatttttt aataagtaaa
     5881 gaagttatta atactgcaca tattactccc attgagtaaa aggattgaat ttttgtaggg
     5941 tcagccatcc catacaattt acccaattcc tgattacaat ttaaccataa tacaaatgta
     6001 gatgtacatg taaatccaat acaaacaaga gctatacttg ttggtgtaaa tttcatcttc
     6061 tctgatttaa ctgttttgcc tttgttatca ataacattat tagctggtgg aaatggtaac
     6121 acagctatca aaatggcatc tactacaata agaatcgctg ttactataaa aataacttta
     6181 aatgatattg aatttgctgc aacaaatcca attataaaag gaagcaaaaa ttgtgctaaa
     6241 gctattgtaa atttagtaaa catatttgct attgttccta aactagcaaa tatctccata
     6301 caagaaggag ttacacaggt atctaagaat gaatttgctg cccctccaat tattgcaaat
     6361 acatatgcta tataaaaatt tggtgaaaat acaattccaa caaagaaaat tgcatgtaga
     6421 aagtttccta taagaccact tacttttctt ccatacttat ctgaaatagg tcctgcaaat
     6481 ggataagata taagtctccc taatcctagt gcagcaataa cagctaaaac aatacttaca
     6541 tccaatgttc catctgataa tgttttagcc ccccacattg atgaaaattc tggtttgtat
     6601 tgtcctaaaa ttgaaattcc aataccaagt aaaaaatatg aaaaatacaa cgctatggca
     6661 gtaggataat attttttatt gttcataatt tttctcccat tcttgttaat ttattaacat
     6721 ttttgtgtac aataaactca tccccataat ggatttaaag tatagtcagt taaagcttca
     6781 aatttaactg gactatgagg caaaacccac tatgacaatt tgtaatttat tattaaaaat
     6841 attgaaatac gtatattttt tcattgttta agatattact cttatctatt taaagcataa
     6901 ttcattaact tcttctacag gcatttctaa tccagtatat agattaaaag cctctgctcc
     6961 ttggtataat aacattccta atccaccaat agccttacat ccattagctt cagcatcttc
     7021 aatcattttt gttttcttag gattataaac gacatctgtt actactaagt cttttcttaa
     7081 aacactagta tcttttatat ttgtttcatt gtcatagggc ttcataccaa taagagttgc
     7141 atttgtcaat atatcacttg attctatctc ctcataaagt ttgtttgtat cttctaaatc
     7201 atatagatta acaacacatt ctggtacatc tttttttata ttctcaactg tttgctctgc
     7261 tcttttataa aaatcatcct taggattaaa gatagatatt tctcttgctc catctaaagc
     7321 acattgtact tgtatagcag ttgctgcacc accagcaccc ataattgtaa tctttttacc
     7381 tttaacttct accccatttt cttttaaatt acgaacatag cctactccat ctgttatatg
     7441 accaactaac tttccattgt catttacaat tgtattacat gctccaatta tacgagctgc
     7501 tggagaaagt tcatccatat attctgtaac agcagattta catggcatag taacattggc
     7561 tccttttata ttaaaagttt ttattgctaa aagtgcctct tttaccttat ctactgtgat
     7621 atcaaaggct aaatatctat aatctaaatc taatttctta aaactataat tatacatcac
     7681 tggtgattta gagtgaccaa ccggtgtacc tattaatgca aataatcctg ttctacctga
     7741 aatttccata actataatct cctcccaata ttatcatttt ctttgcaaat aacctattca
     7801 gttatttaca aagaaaaatt tatttattat ttatatattt aatgctactt caaaagcttc
     7861 ttttgttgca tctaatgccc tacgagcttt tactgcatca ccaacaatat aagtttcttt
     7921 tacaacttct tttataactt cacttaatgg gtcgtaatta cgagaaccca tagcaagtac
     7981 tactgaatca aaacctttta aatcatgctc tgtgccatct gtaagtaaat agtcgacacc
     8041 atcatcataa aatttagaaa cttttgcacc agtaatggta tgtatattat aattttcaaa
     8101 atcattcata aggaatttac ggtgttctga aattacatct gcaccaactt cttctctaag
     8161 ctcaataaca gtaacctcat gttcttgttc tccaaggaag gctgccactt cacatccaac
     8221 cattccacct ccaacaacaa gtactttttt cccacaagat tttttaccat caagaaggtc
     8281 tacagcatga attaatcctg aatcattgat tcctggtata ggaagtacaa gtggagttgc
     8341 tccagtagct ataataacta catcaggtga tttctccttt ataagttcta ctgtagcttc
     8401 tgtattagta catattttta caccatattg attacatttt gaaatataac ttctaactaa
     8461 gttagtaata tctccttttc ctggaggata tgcagctaaa cgcatttgcc caccaagaac
     8521 ttcagatttt tcaactaaag ttacattatg tcctctttta gcacataccc atgctgcaag
     8581 catacctcca acacctccac caacaacaag aacttctttg ctaatttcag ctggcttaag
     8641 ctctgcttca tgtcctaata taggatttgc aagacaggct ataggtttgc cttggaacat
     8701 atttggtaca catccttgta agcatgcaac acatggtatc atttcgtcta gttttccatt
     8761 tttagcttta ttaggcattt ctgggtcagc caagctttgt ctaccgaatg ctactaaatc
     8821 acatcttcct tgtctgacca ttaactcagc aaactgtggc tctgtataac gtccaactgt
     8881 tattactggt atactaacag ctttttttat ttcagtaact aattctgcac tgaatccagc
     8941 atgtaaagtc gtaggagccc acatatattc atcatgtata tgtatactac gtgaaacatg
     9001 aagtccatct attccacaat cttctagata tgctgctatt actgaactat cgtgtacatc
     9061 aattcctcct ggaacatcat cagtgctgtt tatacgacat aatattgcta gagaatttcc
     9121 tactttttta cgtatatttt caatgataag acgaggtaat ctcattctat tttcaaaaca
     9181 tccaccaaac tcatctacac gcttatttgt tctttgtgat ataaaactac taaccagata
     9241 tccatgagcg cagtgaactt caacagcatc tgcaccagct ttttgtgcac gtaatgcagc
     9301 atctccataa gattctataa gttcatatat ttcttctgtt gttattgcta gtggtgtatt
     9361 acgaccattt gatgcaggta ttgcagaagc agcttttaat ggatgtcctg ctactttttc
     9421 atttccttca gaaccagcat gttgtaattg tatagatacc ttcgaacctg catcatgaca
     9481 agcatctatt acatttttaa aactatctat tgtgctatca tcatataaac aaggcttacg
     9541 aggacctcct tttgcttttt tgtctacaac actagattct atagttatta atccaaaacc
     9601 acctaaagca cgctcaagat aataagcctt agaagtttca cttaaagtac catctgtgtt
     9661 tgcaaaattg tttcccatag ggggaactac aaaacgattt ggaactgtca ttgaaccaat
     9721 tcttataggt gtaaacatag aactaatctt cataaggttt tccttccttt caagttttat
     9781 atttaaaagt tttatattat aatactctgt atatttttat attatataag tttattttgg
     9841 agatacttca gaccatgctt tatccaaaac ttttttagtt gcattaaata cagtttcagc
     9901 agcagtggat aaccctttag atattagaga atcagatatt acttctgccc ctatgactct
     9961 tggtgatata ttgtgttctt ttattatttt cacaaattcc tctgtgtttc catatccttc
    10021 acctggagcc aaacggtcat gtagtgattc atccctaagt ttttcataag gagtttctaa
    10081 aacatcacac acttgaattg atactatctt atctgctgga acaggtgcaa gatcctctgc
    10141 tgtctgcttt gctctagccc agtgccaagt atctaatata agcattgcat tttcacgatt
    10201 acatcctttc acaacacgcc atgcagtagc caaatctggt acaccactat atggcataaa
    10261 ctcaagacct ataataagtt cacctgctct gtcacataat tctcccaatg cagttataat
    10321 ttgttcttct ggtatcatct ctaataatcc acaatttata tgtttaacat taaataagtg
    10381 agccatatga taaacatttt gctctttttc tctttgagct tccgttctat cttcttctct
    10441 tccccattga gttatatatt ctacttctgt aactttcata tcatattcat taagaattgc
    10501 aatcatttct tcatctgtaa cacctgcatt ttttgcatca atataatttt cagcacgcaa
    10561 tccaatacca tcaaacccag cttcttttgc aatacgaact ctatcacgga atgaaacttc
    10621 tgttcccaat gtataagaac taattgtaat tggaatatta agattttttt gattattcat
    10681 agcttaaatt tgcccccttt attttcttta taattaatag tatttttgat tttatatttt
    10741 tatttatcct actagtagta ttattttacc ttaaaaataa aagtattttt tgatttctat
    10801 ttatctagat tttataaatt tattgtatca cgagacttta ctaataacaa attgataatt
    10861 tcctattaat tcatagattt tgtttataga ttttatttat aagataattc ttgtattttt
    10921 ctattaggtc gtataaatat atttatctta taattcagac aaaaaacgat aaattttttt
    10981 aaaagctttt attcttactc tataatcata tataataaaa atgtaaaaat atcttaaagg
    11041 ttaacttcta caaatatata aataaaaata cctatgaaag gatgaacaaa aattgaattt
    11101 atatcactta cgctattttg ttacattggc acatttagaa cactatacaa aagcggcaga
    11161 aaatttatct ataacacaac caagcttaag tcatgctatt tctttactag aaaatgaact
    11221 tggggtggct ctatttgaaa aagaaggtag aaatatagtt cttactaagt atggaaaaat
    11281 atttttaaaa gatgtggaaa aatcattaga aattttagat tcaagtgtaa aatcattaaa
    11341 aataactggt actggtgaag gacaaataga cttagctttt cttagaacac ttggtactga
    11401 ttttatccca gacatagtgc acaaattttt aaaatctaat cctgctaaat caatagattt
    11461 taaatttcat actggtgtaa ctactgacat tattcaaggt cttaaagaaa gaaaatatga
    11521 tattgcattt tgttcgaaac ttgaaaaaga aaagggaatt gaatttatac ctgttgccaa
    11581 acaagattta gtactaattg taccatatag tcatccacta gctgcaaagg atactataga
    11641 tttaaaagaa accatacctt atcctcaaat agtatttaat cagagaagtg gtcttagata
    11701 tataattgat gatatgttta aaaaaataaa tcaacagcct aatatagtct atgaagtaga
    11761 ggaagaccaa gtaattgctg gattagttgc aaaaaatttc ggaattgctg tagtcccaaa
    11821 tatgaatatg cttagcttta caaaagtaaa agtaattcag attattcacc caagttggga
    11881 aagaaacttt tacttagcat ttataaaaga tagatatttg cctcctgcaa ttaaaaactt
    11941 taaaaacttt gtcattaaaa acgctcaatt ataatttatt aataaagggg ttatttttaa
    12001 atatcctctt ttattctttt tataaaataa aaaaatctat acttttaaaa gtatagattc
    12061 ctaactgaca gtcggcatcc gagtctcagt aattatcgtt atacaacagc aactggattc
    12121 agttcctgaa taactaggtt acttacatta tatcagattt tttaaaattt gtcaattgta
    12181 tctaacgcat tttttcaagc catggtccaa caggctctac aataggaact tctttaaatc
    12241 cccaatttct tatatcattc acactcgttg gagtatatat tttgtttctg acagatttga
    12301 atattttgtt tttttctcca tattgcatac actttggatt agcttctatt aaccctcttc
    12361 ttgctctgct aagaggatta cataaaatat ctgctatttc taaaccttct ctatagtcag
    12421 catcttcttt tttggcaatt ataaaaccct taaccttatc tcttaattct tcatctatat
    12481 ctaagtatat tgttccatta ttaaatacat caaaaaacgc cttttgtact tttaagtttg
    12541 agacatcatc ccttgactct ataactattc gtgcagattc tacagtttca ttatttacaa
    12601 atgaataaaa attctgaaga acatgtataa aagccacaac atacggttcc tttgaaggtg
    12661 cataatattt ctttaatcta gctttatcta ctgttactga tattatagta cactctaatc
    12721 tagatagaaa taaaggcaac tcatcataga ataacttaat ctgtgaatca tcaaacttct
    12781 caaatgctct tgattttctt gatatatctg tcaaatgtat tcctatgtta ctggttccaa
    12841 aacaatactc tttaaatgca tccatttctt tcctcaaaga acatacataa tcgccttgac
    12901 ttactgcata tttatattca aaaattactc ctgtcaatgt aaatgggtct tcatgattta
    12961 tatcagctct tccactttca tcaataaaca ttatatattt tttctttata atcttactca
    13021 ccctcttaac atatgatgat agcatctata ttcattttat atgagtttta aaaatattcc
    13081 aagattttta tataacttaa acttaatttt atactaaaaa attgagatgt aaaacaatca
    13141 agcttttatt gattgaattt cacatctcaa ttataattat ttttcaatat atatttttac
    13201 tattttgcaa gaatatccat taatttatcc attactatat aagatactcc gcctcctatt
    13261 tgataaggag tttcaacttt tttatttgca aatactcttt gttgctctga tgataatcct
    13321 gaattatcca ttaaaactat tggtgaatgt aattttacag ctaatggtcc agctgataaa
    13381 gcatctacta aagcatctga tttactaact aatacagcct gtaaatcagt atctccatag
    13441 aatttttcta ttactttggc attagtatct actctactat ctccatatat tctatttcct
    13501 gaaatatctt cagtagttat atcattcatt tttgctatta ctgaatcgtt aattgcactt
    13561 ggtccaccta taaagtatgc attttctaat tttctttctg ttagccattt ataagattct
    13621 tctgtaattc catctttatt gactaatatt ataggttgtt tttcttcccc agctttagaa
    13681 gctacagata aagcatctgc ctccccattt ccagaaacca tatatgcagt ttttatttct
    13741 tctcgatttc caagttcttc agctattctt gtagcagttt gatatctatc ttgacctgct
    13801 attctctttg tttctgtgtt gaaagtagtt tttatttctt gctctaattt agaacttatt
    13861 gcattttctc cacctatcaa gattactgtt ttaggtgcta ataacttcat ttcttcaaca
    13921 gtttcagttg gtaaattagc tgtattagta agtagtattg ttgagtcata gctacttgct
    13981 aaaggagttg ctgttatacc atcagctatt gcattaccat ttactaatat tacagtatct
    14041 gagctatcag cattccagtt tccaactgaa cttatctttt tagctgtatc aaatctattt
    14101 gcacctataa ttgtatccca tgtaggattt atagttccat cttcattaaa agtataatct
    14161 ttcccatcta ttttttgaga accagttagc atctttccat ctttgtctaa atagtaagac
    14221 ttaccatcta tttgttgcca tcctgtctgc attattcctg ttttgtcaaa gtaatactta
    14281 ttaccatcta tttcttgcca tcctgttact gctaaaccat cagatcctag atagtatttc
    14341 tttccattct tttcttgcca tcctgtttgc attactccat ctgagttaaa gtaataagtt
    14401 tttccgtcta ttactttcca tccagatact cttgctccat cagttcctaa ataatatttt
    14461 ttgttattaa ttgttttcca tcctgtttgc attactcctg aagcatcaaa gtaatacatt
    14521 tttccattta cttttagcca actattcttt tggtatgttc catcagattt tacataatac
    14581 ttcttaccat tttctgtttt ccatcctgtt tgatttagat ttatcttata ttttgccgct
    14641 atctttacat agctgtcgtt actataatct ataattacat ctgaacctac aggaacttgg
    14701 tcaaataacc atctaacatc tttattgtgc attcttatac atccaccaga aatatgcttt
    14761 cctatagaac tctcattatt atttccatgt attccatatg tatctccata agttcctttt
    14821 aatgctaatc ccatccatct atctcctaat ggatttcttg gacttcctcc aggtatatta
    14881 cctttataat atggtctatt ttttatcttg ttaactactt ttgttttccc tgttggtgtt
    14941 tctgacccct ttttacctgt tgcaactcta aattctttta caagtttatt atttacaaaa
    15001 tatcctagag tattttttct tgaatttact ataatcacat gtttactggc tgcgcttgat
    15061 tctggtacgc cacatgcaaa tactaaaact aaagtcagtg ctagtactaa atactttttt
    15121 agactttttt tcactataac ttccccctct tttagaaaca ttactacatg tgtctaatta
    15181 taacataaaa ttaccaatac cttatattta caaatttgtt acatttatac atataagtat
    15241 gtattctact cttcataccc attaggattc atactttgcc atctccatga atcttcacac
    15301 atctcttcaa gttcatactt agctttccat ccaagctctt tttctgcctt tgaagaatct
    15361 gcataacaca tggctacatc tccagccctt cttcctacaa tcttatatgg tatttctttt
    15421 ccacttgctt tagaaaatgc ttttactaaa tcaagaacac tatacccctt tccagttcct
    15481 aaattataaa caacaagtcc tggattttct tctaattttt gtaatgcttt tacatgacca
    15541 gcagctaaat ctaatacatg tatatagtct ctaactccag ttccatcatg tgttggatag
    15601 tcattaccat aaacactcaa ttccttcaat ttacctactg ctatttttgt tatataaggc
    15661 attagattgc tcggaactcc atttggttct tcacctattc taccactttt atgagcaccg
    15721 actggattaa agtatcttaa aagagctata tccaatgact tatctgcttt tgatatatct
    15781 actaacatct gctctatcat aagtttagtt cttccatatg gattggttac agaaagtggg
    15841 aaatcctcta atataggaca agtatgtggg tctccatata cagtagctga agaactaaat
    15901 acaaactttt taacaccata ttctctcatt aattcaaata aatttaaggt acttattaga
    15961 ttattgctgt aatattcaat tggtttttct actgattcac caactgcttt taaagctgca
    16021 aagtgtataa ttgattcaat attattttct ttaaatacaa catgcatttc atctttattt
    16081 ctgatatcaa tattataaaa ttttactggt tttttagata attccttgat tctatcaacc
    16141 actattgagt tgctattact taagttatca actataacaa cttcataacc tgattctaaa
    16201 agttctattg ctgtatggct tcctatatat cctgctcctc cagctactaa aactgccatt
    16261 gtaattacct cttttctaat atttattttt cacttacttt ttttctcata tagtccatta
    16321 aatcatcttt taaattttct ctctttagag caaagtctat tgttgcctct aaaaaaccaa
    16381 tcttatctcc cacatcatat cttctacctt caaagttata agcataaata gcctcttgtt
    16441 ttccaagagt ttgaagtgca tctgtcagtt gtatctctcc acctttacca ggtgtctgtt
    16501 tctctaatat gctaaatatt gcaggagtta ttatatatct tccaagtata gctatatcag
    16561 atggagcttc ttcaattgca ggtttttcaa ccatatcttt aactttatat actctatctt
    16621 cgatatgttt tacatccaat ataccatatt tattagtatc ttctttagct atcttttgaa
    16681 cacctaatac agttgtttta tactcattat aagtatctat taattgtttt aagcacggaa
    16741 catctgaatc tactatatca tcacctaata atacggcaaa aggttcatca cctataaaac
    16801 tttttgcaca gtaaattgca tgtccaagac cttttggctc tttttgtctt atataatgaa
    16861 tatttaccat atttgatatg tctcttacca tttctaacat ctctgtctta cctttttgct
    16921 ctagttctaa ctctaattca actgatttat caaaatgatc ttctatagat tttttatttc
    16981 tacctgttat tataagaatt tcttcaattc ccgattctat agcttcttct attatatact
    17041 gtaaagttgg tttatctact ataggaagca tttctttggg ctgtgatttt gttgctggta
    17101 aaaatctagt tcctagccct gctgctggta ttattgcttt tttaactgtc atctgcattt
    17161 tttcaccctt cttcgtaaat tttctattgt accaaattaa tataccatat ttatactttt
    17221 tcttcaaact ctatattatc ccaatattta atgctataag ataatataag gtagatgaaa
    17281 tttcatctac ctttagtttt ataagttaat attgtattgt ttatatttta tccaaaactt
    17341 atacaggtac taaatttcat ctaagatgcc ttttatatca ctatctatat cttcatctag
    17401 ataagaatct aaatcttcta agtcaacttc tagttcttga tatgtcatat caacactgac
    17461 atcctcatct atatccattt ctgatattaa ttctcctaaa atactatttc catcagaatc
    17521 taaaccacta tctatatctt catctatata gttttcattt aaaatttcat ctaaatcaat
    17581 ctcataataa acttcattat ttttttcatc ccctgagtcc aatcctaaga actcatagtt
    17641 aaaagtccaa acattgtatt tacttgacat actttattcc tcctgtgaaa aacatatttt
    17701 catctagttt attggttcca taatcaacag agttatatat tttttcaatt atgtcttttc
    17761 ttctttcttt tgtactatta tttaatgtca attgtttaaa tacatatgta tttgttattc
    17821 tctcgaataa tatttctttt atactatcat catcaatatt tctatagttt ttccacaata
    17881 actcaaataa cttaaacatt tccctactag tttcatctac ataataagga tacatatttt
    17941 ctactaaatg gtttaatctt gatatggctt gttttaaaaa tacatcaaga cttgcacttt
    18001 cactttcttt taacattcta taattgtatt tcttcttatc ttctcctgaa attacaaaga
    18061 tgatattgtc taaacttttt tcaacttcat ttaaataact gcttatatta tatgataaat
    18121 cagacaatag atttttaatc tgcttaaaca cttgtacatc aagtttagta ttatactttt
    18181 caaacaaatc tatttcttga ttttctaata ttcttggcat atatgctgac aataaatcta
    18241 aattgtttct ttgttcttgt atttgcttat aaagtgaatt tataacttct attcttattt
    18301 catcatttaa ctctttttct aaaatcttat caaattcaga tttcatatag taaatatttc
    18361 caaagaaatt aaatattatc atatcattca tagctttaga ccaattctta tatatcactt
    18421 caatagaatt ttctttaagt agaggttgct ttgcatatat aaatacatct tgcataagtt
    18481 tttgaaccat acttttactt aagtttaaaa gtactttttg attattaaat tttatttccg
    18541 tttctacaaa attagaattt tcgttttcaa gttctttatt aattacaatg tcttttatat
    18601 cactattttt tatagatatg atttcttttt gaaaagactt tagaatagtc aagctatcga
    18661 aatcttcttg aataaaaaat ggagcatgtt ttaatgtata tcccactatt tcattttcta
    18721 tatcttttag gtcatctatt tgcataattt ctctattttt atcatttata tgtaggttaa
    18781 atacattttt attatcatat aatattatat cttctcctaa atttaactta gaacaattat
    18841 ctttcgctga ctttacaata gtcttcttag atttcttatt tataaaaatt aattggtctt
    18901 gatattttac taaaagcatg tttgtgtttt tataatcaaa tccatctata tttcctgata
    18961 tcttgcagaa tactatttca caatctttag gaagatttcc tattttttgg ttatctttaa
    19021 ctctctcatt taaatcagat gggactacac tatctatatt gttattaaat atagatacag
    19081 accttactat aatcccctct ctttccatat gaaagtatcc cttcaatgac actcttttat
    19141 cttctaaaat gtatctttct atatcatcaa aatttatttc aaatctattt ttcccactga
    19201 taaatataaa cttctcatta tctttattaa ttccacccat ataaaactta ttgtcatcta
    19261 aacttaatat agtcatatta tcctttagat agaatataaa ttctctttga cttttgcaga
    19321 ttaaatcata ttcatccaaa gttattttca tagtcaaatt tgctgtaaca tttttacttg
    19381 aattttcact ttttatgtaa aaaggcaagt tatttccata gacaattatt tgagaatctt
    19441 ctaaaactgc ctctaatttt tctttattgt ctgaaaaact atatatatat tttacacctg
    19501 tatcctcatt aacttcatct gatttgctga ttattatatt ccctagattt gttatatgaa
    19561 gctttttata ttcttttgag tatctactta tgccactaag ctcaaaccca tctgaaagct
    19621 catatatgat tttttctcct aatttcaaca taccttcacc acctaaacag aattataagt
    19681 attttataaa ggtgaataaa atgtctcaaa acaaataata ttttttttaa tactttttat
    19741 tctgagacat agtttctcct tttttataat gctctaagca ccttcaacta tttcttcata
    19801 aagtttatct aaatgtttaa catattcacg atatccagat ttgttcttta actcacttct
    19861 taaatcttca tatttttgtt taagttcttc agcactcaaa gaaggagctg attcttttaa
    19921 ttcttctaac ttataatcat agtacttatc atacatagct atatatgtat caatagactt
    19981 cttagctagt tcattacatt tttcttgtgc ttctccatat tgagaataat cacctatttt
    20041 tattttatag tatccatttt cttgaattat tgttgaagaa atacctttag cctcatattc
    20101 ttttgctgtt tcttttgctg ttttttcaac tttaagtgaa cctgcataaa cagtgtaata
    20161 tacaccttta ccatctttat atgcttccat ttcttttgct ttcttttcag cttgtgcttt
    20221 ttcttgagcc tctttttcag cttttgcctt ttcttctgct tcttttttct ctttttcagc
    20281 ttgtgctttt ttctcttctt cagttaattt attgttagct tgttgttgag ttgatgactt
    20341 tggaatattc tctttatcat ttttcttatt gttgcctact gttataaaat atcctacacc
    20401 aacaataacc actataacta ctgctataat agtttctttt aatctactct tcttagaaac
    20461 aacctcttca tcatctaaag aatcttcttt atctgataca taatttttca tttctttctt
    20521 gttttctcta tatttgtcct tagaagaatt tttagatttt ttattactat tttctttctt
    20581 tttaataggt attactactg atttttcatt ttctaatttt tcttctttta tctcttttgc
    20641 ttttttatct tctaagtttt tactttcttt ttcaagcttc gattcattat ttgaagatac
    20701 tttttcatct aagttattat ttattggttt ttctattttt acattacgtt cttcttctat
    20761 atgttgactt ggttcttttt gttttatttc ttttaattca ttttgctcca tttcttctga
    20821 ttttacttga tgattttcaa ctggtttatc taaaattata tcaggagaaa tatcatttaa
    20881 attctcttct tcttgttcaa cttcttcatt gtctttttta ataaatccaa ataatttagc
    20941 cttcttttta ctctttttat tttctggttc aattatatct tctttattgc tgtcgtactc
    21001 tttatctgta gtatctttat cttctacaac gtcacctata tcttgattat tactatctat
    21061 ttcttcttta accccttctt cagcatcatc ttctattgaa atactacttc taaagcaaat
    21121 atctttatct gcatttttta tcatataaat gtattcttca atatcgatat ctttatttct
    21181 tctagttctt tttgatgcaa tttcatgatt ttctaattca ctatagttat cttttattag
    21241 attctgtgct ttattccatg tatcttcact aatttctgtt ttatttttaa ttacatagtc
    21301 cacaaattcc ttaaaatttt caggaccaaa ttctccaatt atagtcagtc tcattgcacc
    21361 ttctataagg tctctatttc ttctctttaa aaattctaat acaaccgttg cacaagcaaa
    21421 atctttaata atttctttgc caaaaccata tccttcaaca gaatgaagtt cttctaatcc
    21481 acataacttc ttatattcat tgtgtattgt ttgagataca ctttcattat ttaagctatt
    21541 taacttatct ataaacttcg attctatatc atttgatttt gattcatgtt ttacttttga
    21601 atttttaatt tcatttagta gcatgtcatt ttctgaatta tctttaccta attgcaaatc
    21661 ctcaatttca gttgctttta tttctacttt gtcttgttcg ttattctctt ccacattatt
    21721 aggtaccaca tctttattgc tagctaatat tttgtttaca cagaatataa tttctctttt
    21781 taattctctt ttcgcatctg cttgattttt aagaaataaa tctccacatt ctttcttttc
    21841 taaacttatt tttctaagag atatattaag aaggtcttta tataggttta aaaccttata
    21901 atccttttct aaacgtcttc ttctctctga tagttccatt ttatcataag ctaaaaaact
    21961 atttaaatca ttggctttta gagagtctac tccctctttc atagccttat ttacatactt
    22021 tctacatttt gctgtagtta attctaaatc tttttctaat tcatctattt caggtatgta
    22081 cttatctact atccatttgc caataagtgg ttctgtattt cttgctaatg tcttataaaa
    22141 caatatatta aattttaaaa gctctgcata ttttgcttca ttaaattcta ctttttcaaa
    22201 tttcttttcc aaaacaccat ctagcttttc atttatttta gactcttgtt ttaaaaaact
    22261 tgctgtgaca tttaaaacag tgtttcttat tatattattt tcactcaaaa ccttcactcc
    22321 ttaaatcaat tattttattg taatatttac attgtgcaaa gttgctaatt tgattatgat
    22381 atctaaataa ttatttacta aatttgctac atcttatatc atatcatatt atttacatca
    22441 ttttactttt tctttttata tctatcttat attataccat ttttaatatc tgtactaact
    22501 tatattacaa tattcctcaa aattttaaaa gtagattaga caaatttaca aataaaaaaa
    22561 catcagttta aactgatgtt tttgactatc gaaataatta agcaagtcta taagcggagt
    22621 tctgtattag ataatcatct ttctaggcct attgtcacca ataggctcaa gcgacctacc
    22681 taccggagag tacgagcagc actctttatt cctatcttgg tcttgctcca ggtggggttt
    22741 acatagccaa ctagtcgcct agctgctggt gagctcttac ctcgcctttc catccttacc
    22801 acaatagtgg cggtttattt ctgttgcact atccttagga tcgctcccac taggcgttac
    22861 ctagcaccct gctctatgga gctccgactt tcctcatgta agattacatg cgattatctg
    22921 gcttacttaa tatttatttt acttagttat gttaacacaa attcttttac atgtcaaagt
    22981 aatattatac taaaaattta aatttattca aaaatagtgt aatatagata tataaactac
    23041 atttaactcg aggtgatata ttgaaaaaat tatatagaat agtaattaac attatactag
    23101 tattagtaat actttacagt ggatttaata tatactcaaa acttaccaaa tacaatcatg
    23161 atactaaaat atcctcagaa cttcaaaaaa aagaatataa aaaagaggat ttatcaaaga
    23221 ttaacagtga ttttaaattt tggctttctg ttgagaatac aaatataaat tatccagttg
    23281 tacaatctaa agataattct tactatcttg acaaagattt ttataaaaaa gattctattt
    23341 ctggtacact gtttatggat tatagaaata aatctattga tgacaaaaat atcataatat
    23401 atggacataa tatgaaaaat aaaactatgt tcaacaacct aaataagttt aaagatgctg
    23461 acttctttaa aaaaaataat aaaataaaaa taaccttgaa tggaaaagaa tttttatatg
    23521 atgttttttc tgcttatatt gtagaatctg attatgatta tctaaaaaca aattttaaca
    23581 atgaatctga ttatcaaaat tacataaatg atattacttc taaatcattg tataaatctc
    23641 ctattaaggt aaatagcaat gataaaatag ttacactttc tacatgtaca tatgaatttg
    23701 atgatgccag aatggtgatt catggtagat tgatttaaaa ctcacagaca cagtttaaaa
    23761 caataatttt aaactgtgtc atcttttata cctataattt ataaattaga ccactaaaag
    23821 tatttaatac aaaataacaa atacatcaaa caataaaaaa caataaaaaa agactatgct
    23881 aaaaacatag tctttactgg agctggtgat aggaatcgaa cctacaacct gctgattaca
    23941 agtcagctgc tctaccgttg agccacacca gcacacctat tttattgtaa attagtaaaa
    24001 ctaattttta aaagtta
//
LOCUS       DAELJO010000041        23508 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELJO010000041 DAELJO010000000
VERSION     DAELJO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.041"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            262..2280
                     /locus_tag="KPB88_001426"
     CDS             262..2280
                     /locus_tag="KPB88_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0053716.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="KPB88_001427"
     CDS             2785..4797
                     /locus_tag="KPB88_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0053717.1"
                     /translation="MRRNTKLLTTGILSMAIVAPAMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLRLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNEITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="KPB88_001428"
     CDS             5039..6952
                     /locus_tag="KPB88_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053718.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTELR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKLK
                     SKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="KPB88_001429"
     CDS             6945..9164
                     /locus_tag="KPB88_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0053719.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="KPB88_001430"
     CDS             9273..10103
                     /locus_tag="KPB88_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG0053720.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="KPB88_001431"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="KPB88_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG0053721.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="KPB88_001432"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="KPB88_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG0053722.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEKEKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="KPB88_001433"
     CDS             17433..18644
                     /locus_tag="KPB88_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG0053723.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="KPB88_001434"
     CDS             18631..22158
                     /locus_tag="KPB88_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0053724.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGEIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..>23508
                     /locus_tag="KPB88_001435"
     CDS             22289..>23508
                     /locus_tag="KPB88_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0053725.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIE"
BASE COUNT        10136 a         2267 c         4128 g         6977 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tataattatt tataattttg
      181 ggtaaaaaca ctttgatata ttttcaaaag ataaaagatt aaaaaacaag gtagaaattt
      241 atgttaagta aggagattaa tatgagaaga aatacaaaat tattaacaac aggaattctt
      301 tcaatggcaa tcgtcacacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tacaatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacacaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgctc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgccaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1561 tctgcatacc ataaagataa tttagaagct aaatcagcta ataaagtata taaaatagga
     1621 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagata cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggaggaacta ctaagactgt agctacaaat
     2041 atattaaata gaattttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt taagagttgg agtattcatt
     2521 tgtaatacac aatataagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctgcaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgga tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctgaga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagacat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggtt ggaaaaatgg ttcagataaa
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tgcctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatgaaataa ctaaggatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacttaaaa tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaagt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatat gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac atgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatggaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaattga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa cttaagtcta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agccaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgatatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttgtagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatatt atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttggt agctaaacta ttacttgagc
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaag attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaccttgaag
     7501 aaatattatc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatacgtta
     8101 agggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacctttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaatatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct accgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgatatata cttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgagattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaagct attcttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aactcaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 ataataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa gtctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tattttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taacatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatatagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagt tcccatagaa aaataatgct ttacaaaaat tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaagatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaaca tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaga taatcctgac agtgattatt acagtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaaaaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgatgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccacca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacagggaat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacaattagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    18181 ctggttctcc acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tattagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catatgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacggcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat ataacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aagaataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa aattagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt tgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cctagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    20581 ggaaaactag gggagattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa aattaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagggaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt ggcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatccttg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcctt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgaa gttgataata taagattttg tatagaaagt atagaaaa
//
LOCUS       DAELJO010000042        23088 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELJO010000042 DAELJO010000000
VERSION     DAELJO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.042"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            41..1735
                     /locus_tag="KPB88_001436"
     CDS             41..1735
                     /locus_tag="KPB88_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG0053726.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGFCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPCMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2067..2177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2281..2652
                     /locus_tag="KPB88_001437"
     CDS             2281..2652
                     /locus_tag="KPB88_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG0053727.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2748..3695
                     /gene="trxB"
                     /locus_tag="KPB88_001438"
     CDS             2748..3695
                     /gene="trxB"
                     /locus_tag="KPB88_001438"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0053728.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3780..4097
                     /locus_tag="KPB88_001439"
     CDS             3780..4097
                     /locus_tag="KPB88_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG0053729.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4366..5652
                     /locus_tag="KPB88_001440"
     CDS             4366..5652
                     /locus_tag="KPB88_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0053730.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5764..6237
                     /locus_tag="KPB88_001441"
     CDS             5764..6237
                     /locus_tag="KPB88_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5896..5898,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG0053731.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6269..7579
                     /gene="grdB"
                     /locus_tag="KPB88_001442"
     CDS             6269..7579
                     /gene="grdB"
                     /locus_tag="KPB88_001442"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7316..7318,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0053732.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7835..9367
                     /locus_tag="KPB88_001443"
     CDS             7835..9367
                     /locus_tag="KPB88_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG0053733.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9378..10523
                     /locus_tag="KPB88_001444"
     CDS             9378..10523
                     /locus_tag="KPB88_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG0053734.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10833..11912
                     /locus_tag="KPB88_001445"
     CDS             10833..11912
                     /locus_tag="KPB88_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0053735.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12025..12624
                     /locus_tag="KPB88_001446"
     CDS             12025..12624
                     /locus_tag="KPB88_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053736.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12811..13683)
                     /locus_tag="KPB88_001447"
     CDS             complement(12811..13683)
                     /locus_tag="KPB88_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0053737.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14100..15503
                     /locus_tag="KPB88_001448"
     CDS             14100..15503
                     /locus_tag="KPB88_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053738.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15530..17074
                     /locus_tag="KPB88_001449"
     CDS             15530..17074
                     /locus_tag="KPB88_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0053739.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLIVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17105..18496
                     /locus_tag="KPB88_001450"
     CDS             17105..18496
                     /locus_tag="KPB88_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG0053740.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18689..20185
                     /gene="abfD"
                     /locus_tag="KPB88_001451"
     CDS             18689..20185
                     /gene="abfD"
                     /locus_tag="KPB88_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG0053741.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20320..20595
                     /locus_tag="KPB88_001452"
     CDS             20320..20595
                     /locus_tag="KPB88_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053742.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20619..21926
                     /locus_tag="KPB88_001453"
     CDS             20619..21926
                     /locus_tag="KPB88_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0053743.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            21946..23064
                     /locus_tag="KPB88_001454"
     CDS             21946..23064
                     /locus_tag="KPB88_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0053744.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         8980 a         2541 c         4520 g         7047 t
ORIGIN      
        1 tataaataca ataaagtaag gttgtaaagg agtgctttaa atgaagtttt cagagtttaa
       61 gtatgaaaga cccaattata atagtatgaa aaaagagttt ttgagttgtg tagaagatat
      121 aaataattct cgtaattata aagagcaaca aaaaaatata cataagatta atttattaag
      181 aaataaaata gaaacgttgt caaatatagc ttcgataaga tatagtacag acacatttaa
      241 taaattctac aaagaagaaa aaaattattg ggatgaatat atgcctttgt atgaagaatt
      301 aaattcatat ttttataatg ccattgtaaa ttcaaaattt aaatatgatt tgataaaaga
      361 gtttggagag caatttttca caatagtaga gtattcttta aaaagttttt ccaaagaaat
      421 aataagtgaa ttgcaggaag aaaataaatt gtgttcagag tatactagac ttttagcttc
      481 agctgagata atgtttgatg ggaaaataag aaatttatct ggtatgggga aattcatgta
      541 ttctaagaat agaaaaacaa gagaattagc aaacaaagca tattataatt tctttgaaga
      601 aaatgaaact aaatttgatg atatatttga taaattggtt aaacttagag ataagatgtc
      661 taaaaaatta ggttttgaag attttgtaga gcttgggtat gttagaatga tgagaagtga
      721 ctacagagaa tatatgataa aaaatgttag aaaacaagta ctaaaatatg ttgttccaat
      781 ggcaaatgag ctttatgaaa aacaagctaa aagaattggt ctagaatatt taagttacat
      841 agatgaaggc gtggaatttt taacaggaaa tgcaagttta aagggagatt caagatacat
      901 aattcaaaat ggtaaaagga tgtattctga actgtcaaaa gaaacaaatg aattttttga
      961 ttttatgtta gaaaatgaac ttatggattt agaaacgaag aaaggaaaag gagctggagg
     1021 gttttgtact tatataccag attataagtc accattcatt ttttctaatt ttaatcaaac
     1081 tgctgatgat atagatgtat taacacatga ggcaggacat gcatttcagt tgtatatgtc
     1141 tagatggatt gatatgccag aaattaattt tccaacgctt gatagttgtg aaatccattc
     1201 aatgagtatg gaatttataa catggccgtg tatggattta ttttttaaag aagatacaga
     1261 taagtacaaa ttcactcact tatcatcttc aattaaattt ataccttatg gagtaattgt
     1321 tgatgaattt caacactata tttacaaaaa tcctaatgta gataagtcaa aacgaaaaga
     1381 aatctggaga tttttagaga aaaaatactt acctcataga aaatatggtg ataattcttt
     1441 tttagagaga ggttgttggt ggtttaaaca aggacatata tttaaaaatc cgttttatta
     1501 tatagattat gtattagctc aaatttgtgc gttacaattt tggaaaaaaa tgattcaaga
     1561 tagagatgca ggatggaaag attatataaa tatctgtaaa gttggaggaa caaaatcatt
     1621 tttagatatt gtgagtatgg gaaatttgta ttctccattt gatgatggct gtattgagtc
     1681 tattataggt gatgttaaat cttggtttga tgaaataaat gacagcaaat tataaaaaat
     1741 tatgagaatt ttattgtata aaagtgaaaa attaaaaaat atataaaaag tgattatttg
     1801 ttgtgaaaat agtcttgttt ttaactaaat tgataaaata aactaaaaaa gatttgtact
     1861 taaaaaaaaa agaaaataca agaaatatgc aatatcaaca tttggaaaaa aaagtaaaaa
     1921 tgtatacaaa atacatataa aaaaactatt gcaaatttta tagaatgttt atatacttaa
     1981 gtcaacagag atagaaaaaa gctatttata gaaaacaaag gataaattgc ttaattttaa
     2041 attttctgaa aaaaataata atccggatga agagtatggg agactgcttt ataaaaagcg
     2101 accgaaggga caagtttaat cttacttacc caaaagatta gatgaaattc tcaggtaaag
     2161 gtgactgtac ttggacgggg ctctgaaaat gattttaaca gggaagacaa attgggagat
     2221 ttgtttttcc tgttttttat ttatcaagta ataaataaaa gtttgaaaga gagtaaaaaa
     2281 atgataataa ttacgaataa tccaaaagta aaagaagaag ttcaaggcag agaagttttg
     2341 tttaaagaca caacttatat tggaatctta gaagcaagta gagatttgat acatgaaggt
     2401 tatgaacttt tatctcatcc actctatggg agtgtaaagc caaatgaaac accttacagg
     2461 actgttatac taaagaaagg aaatcgtttg gatataaact ctttaacctt aattgaagaa
     2521 gcaatcatca ctgcaagtaa atttcagaat aataaaaaga ctccaaaatg gacagaaagt
     2581 gttcaagatg attttagagt aattgattat gatatatttt ataatacaat acaaagaatg
     2641 cagtatgaat aaaaaaaatt gttaaaaagt tattaaaaca gcctaaatat taaatctaat
     2701 ttaatattaa ataaaatgag aaatttaatt ggggaggata agtcaaaatg gaaaatgtgt
     2761 atgacttagt aataattggt tcaggaccag cagggcttgc agcaggtctt tatggagcaa
     2821 gagcaaagtt aaaaactcta atcctagaaa aagacaagac tggtggacaa attgtaataa
     2881 cacatgaaat tgcaaattat ccagggtcag ttccaaatgc aacaggacca agtttaatag
     2941 ctagaatggt tgaacagtgt aaagagtttg gagcagaaat gctaagagac aacattgttg
     3001 atactgagct agatggagat ataaaagttt taaaagggga aaaggcagaa tatagagcaa
     3061 aagcagtaat aataggaaca ggagcaacac caagaaagat tggttgtcct ggagaaaaag
     3121 aacttactgg taaaggggtt tcatattgtg caacttgtga tgcagatttc tttgaagatt
     3181 ttgaagtatt tgttgtaggt ggaggagaca gtgcattaga agaagcaatg tatttaacta
     3241 aatttgctag aaaagtaaca attgtacata gaagacaagg atttagatgt gctaagagtg
     3301 ttgaagaaaa agcaaaagct aatccaaaga tagagttttt attagataca gtaattgaag
     3361 aaatcaaagg tgatggaata ttagaatcag ttgtatttaa aaataaagtt actggagaaa
     3421 ctcatgaata ctttgcagat gaagaagatg gaacaatggg agtatttgtg ttcgttggat
     3481 tagatgctca aactgattta ttcaaaggta aagttgatat ggatgaaaaa ggttacataa
     3541 taactgatga agatatgaga actaacatac caggcgtatt tgcagcagga gattgtagat
     3601 ctaagactct aagacaggtt gtaactgcaa ctaatgatgg agcaattgca tctatagtag
     3661 ctgagaaata cattgatgaa aaatttggga actagaatct gtaaattaag aaatagtgtc
     3721 tgtaaattta atgaataata tttaaaatta aaataaaaat ttcaaggggg aataagaaaa
     3781 tgttagattt agataaggct acatttgaag aagaagtttt aaatgcagaa ggttttgtat
     3841 ttgttgattt ttggagtgaa ggttgtgagc cttgtaaagc tttaatgcca gatgttcata
     3901 aattagctga gacttatggg gataaaataa aattctgtaa gatggataca acaaaagcta
     3961 gaagattagc tataaaacaa aaagtattag gtcttccaac tatggctata tacaaagatg
     4021 gagaaaaagt agacgaagtt actaaggatg atgctacagt tcctaatata gaaaacatga
     4081 ttaaaaaata tctttaatat gttgataaat tattaacatt aaaagaaaaa atctattttt
     4141 atctgaagat aagaagaaaa aggtttttat gtccaacttt gttaaaaaaa taattttaaa
     4201 taaaataatt tttttacaat aaatattttt tagtaataaa ataaagattt aaggcataaa
     4261 aacacaataa tatatcttta aaaaaataag ataaattgta aaatgatata attttgagaa
     4321 aaactcaaat gcatatacac aaattaaata acaggaggtg caagtatgcg tcttgaatta
     4381 gggaaaatct ttataaaaga tattcaattt ggtgatgtaa cagaagtaaa agatggagta
     4441 ttattcatca ataagcaaga gatgctacaa gaaattggtg gagatgagca tattaaatct
     4501 atagatatag aattagctcg tccaggtgaa agcgtaagaa taacacctgt aaaagatgtt
     4561 attgagccaa gagtaaaagt tgaaggtaat gggggaatct tccctggtat catgtctaaa
     4621 gttgacactg taggagaagg aaaaactcat gctttaaaag gtgtagctgt tgtaactact
     4681 ggaaaaatag taggtttcca agaaggtata atagacatga ctggagaagg agctaagtat
     4741 actccatttt ctaaattaaa taatgtagta gtaatagctg aacctattga tggtttaaaa
     4801 caatacgctc atgaaaaagc tgttagaatg ataggattca aagcagcaat gtacttagga
     4861 gaagtagcaa gaaatcttac tccagatgaa gtttcagtat atgaaactaa acctcttctt
     4921 gaatcaataa aagaatatcc agagcttcca aaagtaggat atgtttacat gcttcagact
     4981 caaggtttat tacatgatac ttatgtatat ggagtagatg caaaacaaat agtaccaact
     5041 ttattatatc caacagaatt aatggacgga gcaattgtaa gtggtaactg tgtttctgca
     5101 tgtgacaaaa acccaagtta tgttcatata aacaatgggg ttgttgaaga cttatatgca
     5161 agacatggta aagatataaa ctttgtaggt gtaattataa ctaatgaaaa tgtttatcta
     5221 gcagataaag aaagatcttc aaactggact tctaaattat gtaaatattt aggattagat
     5281 gcagttatag tttcacaaga aggttttgga aatcctgata ctgaccttat aatgaactgt
     5341 aagaagatag aaatgcaagg tgttaaaaca gttatagtta cagatgaata tgctggacgt
     5401 gatggaggtt ctcaatcact tgcagatgca gatgtaagag ctgatgctgt tgtaacaggt
     5461 ggaaatgcca accaagttgt agttcttcct aagttagata aagttattgg acatttagaa
     5521 gtagtagatg taatagctgg tggttctgat ggaagtttaa gagctgatgg aactattgaa
     5581 gttgaaatcc aagctataac aggtgcaact aatgaaacag gatttggtca tttaactgct
     5641 aaaggatatt aagaagtatt ttattaaacg attgagtaat gttaataaaa catattatag
     5701 ataattataa taagtgttat agatatataa aaatatatta gaaatataat aaggaggatt
     5761 attatgagtt tacttagtaa taaaaaggtt cttataatag gtgaccgtga tggtatacca
     5821 ggacctgcga tagaagaatg tgtaaaaaca gtagaaggag cagaggttgt tttctcatct
     5881 acagaatgct ttgtctgaac agctgctggg gctatggact tagaaaatca aaacagagtt
     5941 aaagatgctg ctgataaatt cggagctgaa aatgttgtga ttttactagg tgctgctgaa
     6001 gccgaagctg caggtcttgc agccgaaaca gtaactgctg gagatccaac tttcgctgga
     6061 ccacttgctg gagttgcctt aggattaagt gtttaccacg ttgttgagga accaataaaa
     6121 tcattatttg atgaaagtgt atatgaagac caaataagta tgatggaaat ggttttagaa
     6181 gttgaagaaa tagaagaaga aatgtctggt ataagagaag aattttgtaa attttaatgc
     6241 ctaaaagcaa aaggggtgaa actaagagat gggaaaacta aaagcagtac attatattaa
     6301 tcagttcttt gctggtatag gtggagaaga aaaagctgat actaagccac atgtggcaga
     6361 aactttacct ccaataagtt tacaacttga taaattatta ggtgaagata ttgaaatagt
     6421 tggaacagta gtttgtggag atagctattt caatgaaaat atagatagtg caagtgaaga
     6481 agttttatca atggtaaaag gttttgaacc acaacttttc atagctggtc ctgcatttaa
     6541 tgctggtaga tatggggttg ctgctggaac aataactaaa gttgtaaaag atgctttgaa
     6601 tataccagct ttaacaggta tgtatgttga aaatccagga gcagatatgt tcaaaaagga
     6661 tgtttatgta gtagaaactt ctgattctgc agcaggtatg agaaaggcac ttcctaagat
     6721 agctaaactt gcagtaaaac ttgctaatgg agaagaaatt ggaactccta aagatgaagg
     6781 atacatagct agaggaataa gagttaacta cttccatgaa gacagaggtt ctaagagagc
     6841 tgttgacatg ttagttaaga aaataaaagg tgaaccattt gaaactgaat acccaatgcc
     6901 aaactttgat agagttgacc caagtaaagc tgtaaaagat ttatcaaaat gtaaaatagc
     6961 tttagttact tctggtggta tagttccaaa aggaaatcca gatagaatag agtcttcttc
     7021 agcttctaaa tatggaactt attctatagc aggagttatg gatttaacag aagagactta
     7081 tgaaactgca catggtggat atgacccagt gtatgcaaat ttagatgcag atagagtatt
     7141 acctgttgat gttcttagag atttagaaaa agaaggtgta attggaaaat tacatgagac
     7201 tttctataca actgtaggta atggtacatc tgttgcaaac tctaaaaaat atgcttctga
     7261 aattggagca gcattagtgg ctgatggtgt agatgcagta atattaactt ctacatgagg
     7321 tacttgtact cgttgcggtg caacgatggt aaaagaaata gaaaaaactg gacttccagt
     7381 agttcatatg tgtacagtag tacctatttc attaacagta ggagctaata gaatagttcc
     7441 aactatagca atacctcatc cacttggaaa tccagcgctt gaccctacag aagaaaaagc
     7501 cctaagaaga ggccttgtag aaaaagcatt aaatgcttta actacagaag tagatggtca
     7561 aacagtattt gaaaaataaa aataacacac acatatatta tatagataaa aatataaaca
     7621 cacactaaaa ataaaaaata actatacaca aaaaaataat atagatttca tgaatggata
     7681 aattggagtt attcattcat gaaatcttaa aaaatgccaa aatacataaa caataataga
     7741 atcaaatata caataaaata caatgattag agcttttaat aagtttaata agattaaaaa
     7801 ataagtgttc ataaaattta tggaggtgca caaaatgact tatccagttt taaaaggggc
     7861 aggctatgta cttattcata ctccagatat gatagtgcaa aatggaagta cttgtacagt
     7921 tgaaagagca acaaatccag attcagaatt tttaaaagaa gtaagtaatc atataagaag
     7981 ttatgaagat gtagttaact acatgccaaa tcaagtttat ataggaaata gaagaccaga
     8041 agaattaaga gatttaccaa tgccatggtg tgaacaaaag attgagggaa ctagaaatgg
     8101 taagtttgga gaaataatgc ctcaagatga gttcatagct ttaatgcaaa taagtgatgc
     8161 atttgattta gtaaaattat cacaagaatt tattgatgaa gtaaagccaa aaatcgaaaa
     8221 taactaccct gaaattgcac catttgtagg taaattaaag ggtgatgata tagaagaagg
     8281 taaagaatta gtagcaacac acatagctga aggattatac catgatggaa aatttgttgg
     8341 ttatgtaaaa agagcacatg atgtagatgt taacttaaat gcccatacaa tgtttgaaaa
     8401 tctagtagta aaagcatctg gagttttatc tgctattcaa atgctaagac atagcaaaat
     8461 agaccctgct gaaatagatt atgttattga gtgttcagaa gaagcttgtg gagatataaa
     8521 ccaaagaggt ggaggaaact ttgctaaatc aatagctgag atagcaggac ttcaaaatgc
     8581 aactggttct gacacaagag gattttgtgc agcaccaact catgcattaa ttcaagcagc
     8641 agctttagta aaagctggca tacataaaaa tgtaatggta gtagcaggtg gagcaagtgc
     8701 taaacttgga atgaatgcta aagaccatgt taaaaaaggt cttcctgtac ttgaagatgt
     8761 tgttggagga tttgcagtac ttgtatctga aaatgatggt gtaaatccag ttataagaac
     8821 tgatttaaca ggtaagcata ctgttggaac tggttcttca ccacaagcag ttatgacagc
     8881 acttataact tcaggtcttg atagagctaa tttaaaaata acagatgttg atgtgtattc
     8941 agttgaaatg caaaacccag atataactaa gccagcagga gcaggagatg ttccagaagc
     9001 aaactacaaa atgatagggg cacttgctgt taaacgtgga gacttagaaa agaaagaatt
     9061 gaaagatttt gtttctaaca aaggattacc aggatgggca ccaactcaag gacatatacc
     9121 atcaggagct ccttatatag gatttttaat agatgattta actacaggaa atagaaatag
     9181 agctatgata gttggaaaag gtagtttatt cttaggaaga atgactaact tatttgatgg
     9241 agtatctttt atagctgaaa gaaatacagg tgttactgaa gagacttcag gaatatctaa
     9301 ggatgaaatt aagaagataa tagctgaatc tatgaaaaaa ctagcattag atatgttaga
     9361 agaatagggg tgaggatatg tcaaaaaaag taatcgcaga tgtattctta gaagtagcta
     9421 atgcaataga aagcggcgaa tttggcaaaa aagtaaaaat tggtgttact acacttggaa
     9481 gtgaacatgg tgtagaaaac atggttaatg gtgcacaatt agcaaaatct aatttatttg
     9541 atattgtgct tattggacca aaagtggaaa ctgaccttga agtagttgaa gttaatgatg
     9601 aaaaagaaat gcatgcaaaa atggaagaat tattagattc aggatatatt gatgcttgtg
     9661 ttactatgca ttataatttt ccaataggtg tatctacagt aggtagagta ataactccag
     9721 caaaaggaaa agaaatgata cttgcaacaa caacaggaac aagtgctact aatagaatag
     9781 aggcaatggt aagaaatgcc atttatggta ttgctacagc taaatcaatg ggtaacaaat
     9841 gcccaaaagt tggaatactt aatgttgatg gagcaagaca agttgagaag tgcttaaaag
     9901 aattaaaaga taatggatat gacatggagt ttgcggattc aattagagca gatggtggat
     9961 gtgtaatgag aggtaatgat ttacttgtag gtgcacctga tgttatggtt actgatacat
    10021 tatctggaaa tatatttatg aaagtatttt cttcatatac tacaggtgga gattatgaag
    10081 ctcaaggctt tggatacgga cctggagttg gagaagacta tgatagaaag gtacttatag
    10141 tatcaagagc atcaggttct ccggtagtag caaatgcttt aaaatacgct tatgatgttg
    10201 taaagggaga tataagtaat gttgcaagaa atgaatttgc aaaggttaaa aaagcaaaat
    10261 ttgatgacat tatatcctct cttactaaaa aagaagttaa agctgaaaaa gtagaagtta
    10321 aaatgccaga taaggaaatt gttactcgtc aaatagctgg tgttgatata atggatttag
    10381 aagatgcagt atctgagtta tggaaaaacg gaatctatgc tgaaagtggt atgggttgta
    10441 caggtccaat agttttagta aatgaagcta aaggagactt agcagttgaa actttagtta
    10501 aagctggata tactgcaaaa taagtacatt ttttatatgt aaataagaac atatagttat
    10561 aattttattg attataatta tatgttcttt ttattttata tcttgtcaat atttatatat
    10621 taacacaatc catttatttg attctctagc taatttatta tatttattca attaaaatta
    10681 agctttctaa ctaaataaaa actaatgaag tattaaaatg aaattaataa aatatgaaaa
    10741 aattttagga ataaagtatc tgttagataa aaataagatt aattagtgat ataatataat
    10801 taagataaaa tttattattt ggaggtaaaa tcatgagcag agtaaaaaat gtagtagaat
    10861 tgttggaaac taaaggtgtt gatgcactat atctaacaaa gaaaactaat gtaaactata
    10921 taagtggttt tcctgatgaa gaagcatatg cagtgatttg caaagatgga aactttttag
    10981 ttactgatag tagatatatg gagcttgcag aaaaggtttg caaagatttt gaaattataa
    11041 attggcataa ttttgataga agtgtggcaa aggcagttaa aagtgtatgt gataaagtgg
    11101 gtataaagaa acttggattt gaaagaacta atatagtgtt tgataaatat gaagaactta
    11161 aaaatctgat tgagaaggat aatggagaat taatacctac agaaaatatt gtagagactt
    11221 tgagatatgt aaaagacaaa gatgaaatca aaaatacaag aaaagcttgt gaaatagcgg
    11281 ataaggcatt agaagaattg attccacata taaaagctgg agttagcgaa attgaactgg
    11341 ctacaaggtt agagtacttt atgaaaatga acggagcaca aaatataggg tttgaaacta
    11401 tactgatatc aggagcaaag acttcattgc ttcatggtaa gcctagtgat aagattatag
    11461 aaaaaggtga ttttgtactt atagactatg gtgctatgta taatggctat atatctgata
    11521 caactagaac ttttatagtt ggaggagctt ctgaaaagca attagaaata tataatttag
    11581 ttaaggaagc tcaaaatgta ggggttgaga acatgaaagc tggtgtacat gcgacaattc
    11641 cagatgctga gataagaaaa gttgtcaaaa aatatgaaga ctattactat caaggtatag
    11701 gacatggagt cggtagagat gttcatgaag aacctttcat tggtaattat ggtgataaaa
    11761 tcatagaaga gggatgtata ataacaatgg aaccaggtat ttatttccct ggatggggtg
    11821 gagttagaat agaagatact gttttgatta ctaaaaatgg tccagaaaga cttactaaat
    11881 tccctaaaga tttgatgatt ttagacaaat aatttaattt atatatattt ttttacataa
    11941 aatataacat aatctcacta ttaagctcta tttacatatt atagtaaaga gggcttaatt
    12001 ttattgtgag aggtgatagt atatatgtat aaaaaacata aatttataaa aaatataaaa
    12061 aaagcctggt ataaaatcaa attaattgat agatgcctta taattgtaat ggcaatatta
    12121 atgtttcaat ctatatataa tttatttgtt aatgaagtaa attcacaaga tactacaact
    12181 attgatgtag tagtaagaac cacttcagca gctatatttg ggtatttttt aagtgctaat
    12241 tttataaaga gacctttaag gaagtataca aatactgatg tatcttcaaa tctttttata
    12301 gataataatt tatccccaaa aggtcaagag tctaatcaaa ataatataat gaatgtcaaa
    12361 aataccattg gatttacttc agaagctgat aattatgaaa agaaaatacc tattaataat
    12421 aatgaaggtt ttgaagaagg agaaactagt gaattgcaaa ttattatagc tactgtaatt
    12481 tgtatagtag cattaacagt actttttgtt gtaagaaatt ttacaactac aacatcagct
    12541 tctttaggta ctatttcaca gatgagagat tttgtctcag gatgcgtggg ttttttgcta
    12601 ggttgtccaa gtaaacctga ttaaaaaagt attatcaata aaaggtattt taaaaagtat
    12661 cagtataata atatttagat ttaaatgtat acaattataa ttctgtaaca ttctatattg
    12721 tatttttata ctgatactct aaactatact atttattaaa atattttaac attgtattta
    12781 aacaaaatat tatatctgta taatataaat ttagtttata tgaatagatg ccatatactc
    12841 aataaaaagt ttcatactat gagatatgta ttgatttctt ctccaaagaa ccccaatatt
    12901 tgcagtaatt gcacgttcta atggtattga acagagattt tctgtatttt gaaatatttc
    12961 tttatacaat attgttgaag ccaagtcatt ttctagtaaa taacgtatgg tagaaagttg
    13021 atttgagtgt aaaattatat ttggcttagc tttttcaagt ctttttgtaa taatcttata
    13081 gtgataagaa ccttcagata agattacaaa aggtacattt tttaacatat aattcgatat
    13141 aatgggatgt tttgccaatt catttttggg atttgtaact aagtacaatt ctgttttata
    13201 gattgtcttt gaatctatat catcataaat atcactgtca attataccaa tacaaaaatc
    13261 aagtgtagat tcttcaatca ttttagctcc aacaaatgtt ggaacttcaa aaattttaag
    13321 ctctatatct ggataaatta cattaaatct aggaagtatt tttgaaagca aaaacgtacc
    13381 taaaactgta ggaataccta ttttgagaga agcttttctc ttttttccta aatcaagtgc
    13441 gtcacattga aaatcctcaa attgttttat aaaatgtttt gacttgtcta aaaaaatttt
    13501 tccttcaggc gttaactcta ttttattacc tattctgtaa aaaagttcaa agccaagttc
    13561 tttttctaat tctttaattg ctaatgttat ggatggttga gaaatatgaa tactttccgc
    13621 agccttagtc atgtttttat atttacagac agtttgaaaa tagtataaat agtttatatt
    13681 catttggtat tagttccttt catttacata ttagtgacaa gtatataatt aacaatagca
    13741 taagttttgt atattgttgt aaaaaaatag aatagtttta aactatcata gcataagttt
    13801 ttgatatttt caatctattg agaatagtgc tattattaaa ttaacaaaag agaatagctg
    13861 gtatggttta gagtaaatca atctatatta cagttatatc aacgaatatc aaaaaattat
    13921 ctattagaaa ataaaaatta tttttacaat taaattgtta attaaatcat aaagaaaaaa
    13981 gtgatattaa caagataaaa tttttttaat cagaaatgtt aatttaataa caaaatagag
    14041 tattggggag aaaggagaaa tatatttttt aataatattt gttaatttaa taacgaaaaa
    14101 tgaaagggag tgatttagtg aagtttgaat tgatggattt tttaatgaat ccttttgtcc
    14161 ttatgtttgc tgcagtaatt acaggaattt tatttggaaa aattaaattt gggaagttta
    14221 attttggggt atcaggagca ttatttacag gattatttat tggatggctt gcatatagct
    14281 tggggaatct aataatagca aaaggggaaa cagcagcagg atataaggca gcaactgtaa
    14341 tgatggggaa tggaattatt tcttctgact tctttgattt ctttttaata atatttgttg
    14401 cagcagttgg tctattggca gcaaaagata tgaaagctgt attaaagaag tatggagcaa
    14461 gatttgtaat tttaggagtt cttataactt ttataggtgg ctctatgact tatgcaatga
    14521 ctttactatc aagtgataaa ggaagtagtg cttatgaagt ttctggcgtt tacacaggag
    14581 cattaacaag ttcgcctgga ttagctgctg ctttagaaac agctggtaaa catgcagaag
    14641 atgtatcaaa agaatttgaa aaagcatcaa taaaagataa aaaagaaatt ttaaaagtag
    14701 tcgacccaga aggcaaattg gatgtgaata caactacatc actgactcaa gagcaaatag
    14761 ataagtatat tgcatatgct gaagctggtg ttggtatagg tcatgcagtc gcatatcctt
    14821 ttggagtttt aatagtaatc ttaggtgtaa atttccttcc aaaattattt aggatggacc
    14881 taaaagaaga aagaagaaaa tatgagaagg aaatgaagga agcaagagat agtgtttctg
    14941 gtaaaacgat accagaagtt ccatttaata taataacatt tttcttgaca tgtttagctg
    15001 gatacttagt tggaggaata catgtattta tgggaccact agggtacttc acactaggtg
    15061 caacaggagg ttcactaatt gtatctctag ttcttggata tataggtaag ataggggtag
    15121 taaatttcag aatggaagaa aaggttttaa atattttaaa acaaataggg ctagtatttt
    15181 tcttagcaat agttggatta agatatggtg gaaaagtagt tgattcaatt atgacatctg
    15241 gtatgcatct agccttagta gcaatagcag taggtgttac agctatgatg ataggattct
    15301 tagttggcaa atacgtattt aaattaaatt ggattttatt atctggagct gtctgtggtg
    15361 gtatgacatc aacaccaggt cttggagctg ctgttgatgc tcttgatagt gatgacccag
    15421 cagcaggtta tggtgctacg tatccatttg cattattaac taaagttata ttggttatag
    15481 ttttacacaa acttccaatg taaataagat tttatatggg gggatattta tgaataatga
    15541 aggagcattt gaagaattaa gtggaagatt aagaaataaa gaattgtgtg atttaataat
    15601 gaatgcagat gaagctgcta aaattataga ggatggcatg gttattggag ttagtggatt
    15661 tacaccttca ggctatccaa aagcagtacc attagcagtt tcagaaagag ctaaaagtgg
    15721 agaaaagata aaattaatcg tatattcagg agcatcttta ggtccagaag ttgatggagc
    15781 atggtcagaa gcaggtataa ttgaaagaag acttccttat caaactaatt caatacttag
    15841 aaataatatt aataaaggtg ttgttgatta tatagatatg cacctaagtc attctactca
    15901 atttttaaat tatggaacta tacctaaagt agatgtagct atagtagaag cacttgcaat
    15961 aactgaagaa gggaatatta taccaactag tgggataggt aattctccat cttttataaa
    16021 aagtgcagat aaagtaatag tagagataaa tcttgcaaaa ccaatggaaa tggaaggtat
    16081 ggcagatata tatattacgg aaaatccacc aaatagaaaa ccaatagaaa taaatcatcc
    16141 taaggataga attggaacaa catatatacc ttgtggtttg gataaaattg caggagtagt
    16201 aattaccaat atgcaagata agacccgtcc attgggggtt gtagatgaag cttcgaaaaa
    16261 aatatcaaat aatattattg cttttttaag agaagaagta aaggcaggca gattatcaaa
    16321 gaatttactt cctcttcaat ctggtgtagg aagtgtagca aatgcagtat tatatggatt
    16381 atgtgaatct gaatttgaaa atttaacttg ttatacagag gttgtacagg actctatgtt
    16441 ggatttaata agaatgggta aagttacaat ggcatctact acatctgtaa gtccatctcc
    16501 agaaggatta ataaaatttg aaaaagatat tgattttttt aaggataaga ttatattaag
    16561 acctcaagaa attagtaaca atccagaaat agccagaaga attggtgtaa ttgcaatgaa
    16621 cactgcaatt gaagttgata tttatggaaa tgtaaattct actcacataa tggggtcaaa
    16681 gatgatgaat ggaattggtg gttctggtga ttttgctaga aatggagcaa taactatatt
    16741 tagtactgag tcaattgcaa aaaatggtga tatatcatca atagtaccta tggtatctca
    16801 cgtagaccac actgaacatg atgtaatggt aattgtaaca gaacaaggat atgctgattt
    16861 aagaggtctt gcacctagag aaagagctat aaaaataata gaaaattgtg cccatccaga
    16921 ttataaagaa caacttaggg attatttaaa tagagcttgt aaaagtggag caaaacaaac
    16981 acctcacata ttggacgaag ctttatcatg gcatagtaaa tttatgagta caggcacaat
    17041 gaaaaaggct gaaactttta aaagtgcatt ataaaatatt taataaattg gaggtagatt
    17101 ttaaatggaa aaagcagtgg aaaactttga agatttaagt aaggaataca ttaatggtta
    17161 tattgagaga gcaagaaaag ctcaaagaga gtttgaatgt tatactcaag aacaagtaga
    17221 taaaatagta aaaatagttg gaaaagtagt gtattataat gctgaatatt tagctaaact
    17281 tgctgtagaa gagactggaa tgggagtata tgaagataag gtagctaaaa ataaaagcaa
    17341 agcaaaagtt atatataaca atttaaaaga taaaaaatca gttggtataa ttgatataga
    17401 tagagaaact ggaataacaa aggtagctaa accagttgga gtagtagcgg caataactcc
    17461 ttgtacaaat ccaatcgtta cacctatgag caatgcaatg tttgcactta agggaagaaa
    17521 tgcaattatt atcacacctc atcataaagc aattggatgt agtacaaaaa ctgtagagat
    17581 gattaatgaa gaattagaaa aaataggagc tccagaaaat ttaatacaaa tacttgacca
    17641 acaatcaaga gaaaatacaa gaaatttaat ttcatcagca gatgtagtaa ttgccactgg
    17701 aggaatggga atggtaaaag ctgcgtactc aagtggaaaa ccagcacttg gtgtaggggc
    17761 aggaaatgtt caatgtatca tagatagaga tgtagacatt aaagaagcag tacctaaaat
    17821 catagcagga agaatttttg ataatggtat catttgttca ggtgaacagt cagtaatagt
    17881 agcagaagaa atgtttgata aaataatgga tgaatttaaa aataacaaag gttttatagt
    17941 tagagataaa gtacaaaaag aagctttcag aaatgctatg tttgtaaata aatctatgaa
    18001 taaagatgca gtaggtcaat cagttcatac aattgcaaaa attgcaggtg ttgagatacc
    18061 agaagataca aaaataatag taatagaagc tgatggacca ggagaagagg atattattgc
    18121 aaaagaaaaa atgtgtccag ttatatctgc atacaagtat aaaagttttg aagaaggtgt
    18181 agctattgca aaagcaaatt taaatgtaga aggaaaagga catagtgtat ctatacattc
    18241 aaatacagtt aaaaatatag aatatgcagg agaaaatata gaagtatcaa gatttgttat
    18301 taatcaatgt tgtgctacta gtgctggagg aagtttcttt aatggtcttg cacctactaa
    18361 tactctaggt tgtggttctt ggggaaataa cagcatctct gaaaatcttg attataagca
    18421 tctaattaat atttctagaa ttgcttatta tatgccagaa aatgaagttc ctacagatga
    18481 agaactttgg ggataaaatt tagacaaaaa atattcattc taaattaaag taggaatata
    18541 ttttaattta gaatgaattt aagacataaa ttaaatttaa attagtttat tggactaaat
    18601 ttaaaatatt aagagacata tagaagaagg aagtattata atttaaaata aataaaaaaa
    18661 tctataaaag agaaggagtg gttttattat ggcattaatg acaggagctc aatatattga
    18721 aagtttgaga aaattaaata ctaaggttta tatgtttggt gaagaggtta agaattgggt
    18781 tgaccatcct atgattagac catctataaa ttgtgtagca gcaacttatg acttagctca
    18841 tgacccagaa tatgcagatt taatgacagt tacttcaaat atcacaggtg aaaaaataaa
    18901 tagatttggt catcttcatc aaagtgttga tgatttgata aaaaaagtaa aaatgcaaag
    18961 actatgtggt caaaaaacag cttcttgttt ccaaagatgt gttggtatgg atgcgtttaa
    19021 tgcagtgtat tcaacaacat ttgagtgtga taaagcccat ggaactaatt atcatgataa
    19081 ttttgtaaaa tacttaactt atatacaaga aaacgattta gtagttgatg gagctatgac
    19141 tgaccctaag ggagatagaa gtctatcacc tagtgctcaa cctgacccag atatgttcct
    19201 tcatatagta gaaagaagag aagatgggat aattgtaaga ggagcaaagg cacatcaaac
    19261 tggttcaata aattcacatg agcaccttat aatgcctact ataagtatga ctgaagcaga
    19321 taaagactac gctgtatcat ttgcagttcc ttcagatgct gaaggagtgt ttatgattta
    19381 tggaagacaa tcttgtgaca ctagaaaatt agaagaaggt gcagatgttg atttaggaaa
    19441 taaggagttt ggaggtcaag aagctttagt agtatttgat aatgtatttg ttccaaatga
    19501 tagaattttc ttatgtggag aatgggattt ctctggtatg ttagtagaga gatttgcagg
    19561 ataccataga caaagttatg gtggatgtaa agtaggagta ggtgatgtaa taataggagc
    19621 agcagcttta gcagctgatt ataatggagc aaacaaagct tcacatataa aagataaact
    19681 aatagaaatg actcacctta atgaaagctt atattgctgt ggtatagcat gttcatcaga
    19741 aggtcacaaa acagaagctg gaaactatca aatagattta cttcttgcaa atgtgtgtaa
    19801 acaaaatgta actagatttc cttatgaaat agtaagactt gcagaagata tagctggagg
    19861 gcttatggtt actatgcctt cagaaaaaga ctttaaatca gaccttaaag ttggaacaag
    19921 tggaatgact attggcgaag tatgtaacaa gtattttaaa gcttcttcag tggcttcaac
    19981 agaagaaaga atgagaatat taagattctt agaaaatata tgccttggtt catctgctgt
    20041 tggttataga actgagtcaa tgcatggggc aggttcacca caagctcaaa gaataatgat
    20101 ttcaagacaa ggaaacataa atcaaaagaa agaattagct aagaaaattg cgggaataaa
    20161 aaaagaggaa gctttaaatt tataaaaagt tgatatataa agggaaatta tatattaatg
    20221 tgtattaatt aatgaaaaaa tatgtagtat aagagatagt tataaaatta tggtggtttt
    20281 tagctaagta aagaaatttt taataaggag aatttacaca tgacctgtgg agttagattt
    20341 tgtgggggct gtaaccctag atacgaaaga gggaaggctc ttacaataat caaagagcac
    20401 ttcaatggta gggtagattt tagtatagca caagagaacg tcgagtatga tttgctactt
    20461 ataattggag gatgcgcaac tctatgcgca tcctatcaac aatataaagt gaaaaatgaa
    20521 tgtgttaaga tgtgggattt tagccatata gaaagagtta taaatgtgat tgaatcagca
    20581 atttgtgaaa cttaaaaaag ctaaatgggg ggatttcatt gagttggcaa gaattgtatc
    20641 aaagtaaatt atgtagtgct acagaagctg taaaacagat taaaaacggt gatactgtag
    20701 tatttgcaca ttgtgtaggt gaacctcctg cactagtaga ggctatgatt gaaaatgctg
    20761 aacaatataa agatgttgag attaaacata tggtaagctt gggtagtggt ggatatactg
    20821 ctaagggaat ggaagctcat tttagagtaa atccaatgtt tgtaagtgga aatgtaagaa
    20881 aggcaattga aaatggagat ggtgatttta cacctgcatt cttccatgaa gtaccaaagt
    20941 tgcttagaga aaaaagatta aaatgtgatg ttgttttagc acaggtaact ccaccagatg
    21001 aacatgggta ttgtagctta ggaacatcag ttgattatac ttatgaagcc attaaaactg
    21061 ctagaacagt aattgttcaa gtgaatgacc agtttcctag aacctatggt gaggtagtcc
    21121 atgtatcaga gtttgactat atagtggaaa aatcacaacc tttatttgaa ctacaacctg
    21181 caaagattgg agaagtagaa gaagcaattg gtaaaaattg tgcttcatta attgaagatg
    21241 gttctacttt acagctaggg attggtggga taccagatgc agttatgtta tttttaactg
    21301 ataaaaagga tttagggatt cattcagaaa tgatttcaga tgggactttg gccctttatg
    21361 aaaaaggtgt tataaatggt aaatataaaa attttgataa agaaaaaatg acagttacct
    21421 tcttaatggg tactaaaaaa ttatatgact ttgcaaataa taaccctgca gtagaggtaa
    21481 aaccagtaga ctatgtgaat catccagcaa ttataatgaa acaacataag atggtttcta
    21541 taaattctgc tatacaagtt gatttaatgg gacaggtagt tgcagaggct atgggactta
    21601 gacaatttag tggtgttgga ggtcaagttg actttatacg tggagtgtct atgggtgaag
    21661 atggaaaggc aattatagct atgccttcaa taactacaaa aaaagatggt acagtaattt
    21721 caaaaatcgt atctattgtg gatgaaggtg cacctataac gacatcaaga aatgatgttg
    21781 actatattgt aacagaatac ggtatagcag aattaaaagg aaaatctctt agagaaagag
    21841 caagaaattt aataaatata gctcatccaa gtgtaagaga aagtctagca gtagaatttg
    21901 aaaagagatt taaggagaaa tattaataat agaggggtgt taataatgga agcacttaga
    21961 gtagttccaa agatatttta ctttgataca tttaaagaat ttaatgaaga atttaaaata
    22021 ggaaaaaatg atttagtaat tactaatgag tttatatatg agccatatat gaaacctctt
    22081 ggaatagata caaatttaat ttttcaagaa aagtttggaa caggtgaacc atctgatgaa
    22141 atgatagact ccatgacaaa ggaaatgaag aaatacaatt ttgatagaat tattgcattt
    22201 ggaggaggaa caatagttga tatatgtaag atattagcac ttgatgttcc agaaaagtct
    22261 attgatttat ttgaaggaga agtatcacct aaaaaagtaa aagagttagt agtagttcct
    22321 acaacatgtg gtacaggtag tgaagtaaca aatgtagcta ttgcagagtt aaaatctaaa
    22381 catactaaaa aggggcttgc agtagaagaa acatatgcag actatgcagt gttaattcca
    22441 gagacagtaa aagggttacc ttacaaattc tttgttacaa gttcggtaga tgctcttata
    22501 catgctattg agtcttatct ttctccaaaa gcaagtccat ttacagagat gtattctcta
    22561 caagcaataa aaatgattat ggatgggtat aagaagatag ttgataaagg agaagaagag
    22621 agatttaatc atcttagaga ctttgtactt gcatcaaatt atgcaggaat tgcttttgga
    22681 aatgctggtt gtgcagcagt tcatgcacta tcttattcaa taggtggagc tttccatgtt
    22741 gcacatggtg aagcaaatta tcagttcttt actgaagtat ttaagatgta ttctagaaaa
    22801 aaacctaatg gaaaaattat aaaatgtaca aaaattttag cagatgccct tgaatgtgac
    22861 cctaactgtg atgtttatgg agaattagaa aagtttttaa ataaattaat agctaaaaaa
    22921 gctttgagag aatatggaat ggtggaaagt cagatagatg aatttacaga tagtacaata
    22981 gcaaatcaac aacgactact tgctaataat tatgtagagc ttagtagaga ggaaataaga
    23041 gagatatttg caaatcttta ttaatacaat tttttattta tatgtata
//
LOCUS       DAELJO010000043        23035 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELJO010000043 DAELJO010000000
VERSION     DAELJO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.043"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            121..459
                     /locus_tag="KPB88_001455"
     CDS             121..459
                     /locus_tag="KPB88_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG0053745.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(586..1875)
                     /locus_tag="KPB88_001456"
     CDS             complement(586..1875)
                     /locus_tag="KPB88_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0053746.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1955..2659
                     /locus_tag="KPB88_001457"
     CDS             1955..2659
                     /locus_tag="KPB88_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG0053747.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2751..3797
                     /gene="selD"
                     /locus_tag="KPB88_001458"
     CDS             2751..3797
                     /gene="selD"
                     /locus_tag="KPB88_001458"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2799..2801,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG0053748.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3815..5227
                     /locus_tag="KPB88_001459"
     CDS             3815..5227
                     /locus_tag="KPB88_001459"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG0053749.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            5341..7224
                     /gene="selB"
                     /locus_tag="KPB88_001460"
     CDS             5341..7224
                     /gene="selB"
                     /locus_tag="KPB88_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG0053750.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7329..10067
                     /locus_tag="KPB88_001461"
     CDS             7329..10067
                     /locus_tag="KPB88_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG0053751.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10186..11130)
                     /gene="manA"
                     /locus_tag="KPB88_001462"
     CDS             complement(10186..11130)
                     /gene="manA"
                     /locus_tag="KPB88_001462"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG0053752.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYILKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11303..12664
                     /locus_tag="KPB88_001463"
     CDS             11303..12664
                     /locus_tag="KPB88_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0053753.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12752..14641
                     /locus_tag="KPB88_001464"
     CDS             12752..14641
                     /locus_tag="KPB88_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0053754.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14656..15111
                     /locus_tag="KPB88_001465"
     CDS             14656..15111
                     /locus_tag="KPB88_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0053755.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15170..15487
                     /locus_tag="KPB88_001466"
     CDS             15170..15487
                     /locus_tag="KPB88_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0053756.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15512..16774
                     /locus_tag="KPB88_001467"
     CDS             15512..16774
                     /locus_tag="KPB88_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0053757.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16879..17943
                     /locus_tag="KPB88_001468"
     CDS             16879..17943
                     /locus_tag="KPB88_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0053758.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17997..19175
                     /locus_tag="KPB88_001469"
     CDS             17997..19175
                     /locus_tag="KPB88_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0053759.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19366..20424)
                     /locus_tag="KPB88_001470"
     CDS             complement(19366..20424)
                     /locus_tag="KPB88_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG0053760.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20491..20862
                     /locus_tag="KPB88_001471"
     CDS             20491..20862
                     /locus_tag="KPB88_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG0053761.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20899..21573
                     /locus_tag="KPB88_001472"
     CDS             20899..21573
                     /locus_tag="KPB88_001472"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG0053762.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21822..22661
                     /locus_tag="KPB88_001473"
     CDS             21822..22661
                     /locus_tag="KPB88_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0053763.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22905..23001
                     /locus_tag="KPB88_001474"
     tRNA            22905..23001
                     /locus_tag="KPB88_001474"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22939..22941,aa:Sec,seq:tca)
BASE COUNT         9203 a         2457 c         3958 g         7417 t
ORIGIN      
        1 aaaattatta ttaaaatgat aattaatatc aataagtatt gacaaaatta tttctaaata
       61 ctattatata taatataaat aataatgaaa atcaatatca aataaatggg aggaaatcta
      121 atgaacttat tagtgattgg tggtcatgaa agaatggaaa aagattatat ggttatggcc
      181 aagaaaaaag gttataagac aaaggtatat actaccatgt catctaaatt aaataattct
      241 ataggaagac cagatgcagt tgtcatactt acatcaactg tttcacataa gatgtctaga
      301 acggttgagt ctcaagctaa aaaacaagat atattaatcg taaggcataa aaatagtagt
      361 aaggtagcat ttaatgaatg tctagatatg gttgatgaat gtctgggaaa ttgttcaaaa
      421 tgtaagctta aagacaaaca aaaacaaaaa aatatatagg tataaaaaat acataaacat
      481 aaaattaaaa gtactgagat aatagttata attattatct cagtactttt agatttataa
      541 ggataagtat agtataaatt ttttattata atttccaact acttgttact ttttcatatt
      601 ttttttactt ctagcttttt ttcttttttt cttctttata tttgaagcta ttataaataa
      661 aactaaaagc actagaatac ctgcaataat cttaacaaaa gtcattaaaa cattattttc
      721 agtcaaaaat ggaagactgc tatcaagatt attttttgca attaagtcta tactgttttc
      781 tagtttacca ttattatata catttaaagt accaacctta gctccttttt ttataggcaa
      841 atcaatttta tttaactctg ttttggtatc ataattgcct tgagattcat ctttttctaa
      901 gaatatccta taactatttt ttggttcata aatcaattca tgttgttttg taaacaatac
      961 ttttttagta tctgtgtatt tttctttatc aacaatagta gcacacttaa agttatcaaa
     1021 tccataatca atcagaatac gtgagtctaa atataaatca gcattagttg aattaaatac
     1081 agcaacgata actcttctgc catcttttac agcactcgat aaaagacatt taccagcagc
     1141 atcagtatat ccagttttta taccatcaac tatatcatat tttatattta taggttgacc
     1201 tttgtatgta atcttatcat gtgatgtcaa aaacttatta gtgtttacaa agtatctctc
     1261 ataaggatat gcttttgtag cttcaaattt aagtgactta gtttttacaa tttgtctaaa
     1321 agtatcatta ttcatagctt ctctagctat gagagccata tcatgagctg ttgtgtagtg
     1381 atttgggtct ggtaatccat gaggattatt aaaatgagta ttagtagctc caattttttt
     1441 tgcctcagag ttcatgaggt caataaattt ttgtacattg ccaccaccaa catatctagc
     1501 taatacaaaa gcagcatcat tagcagaatg aaccaaaaga gcatctagta actgctttac
     1561 agtaaaagat tccccatttt ttagatacat actagagcca tctatttgag gtaaatcttt
     1621 aatttctatt acttgattta aatctttaac ctcctttaga actaaacaag ctgtccatac
     1681 tttagttgtt gaagctggat aaagcttaga atttccatcc ttattatata gtattttgcc
     1741 agactcatag tccattaaaa ctgcatgttt agcaactata gctggttcat tatctgcaaa
     1801 agagatatta gagcttccta aaaatgaagt aaatatcaat aaacagatta ataaaagtga
     1861 taaatttctt ttcattgtga gcctccaaat tttaaattca tcttttagta tagcatatat
     1921 ttgtcaataa ttaaaagaaa ggagtgatta atatatgaaa aaaaaagtaa aatttatttc
     1981 gttttttgtt ttatttattt taataatatc atcaataatc gtttataaat cgcttatttt
     2041 caaagatgat atttatgttg taagtaaaaa aaatttacct aatgaaaaaa cacatgttgt
     2101 agagaatgaa attaaaggtg aagataataa tcacaataaa gtaactaaag acatagataa
     2161 aaattttcaa aataataaaa taactatata cataagtgga gcagtaaata gacctggaat
     2221 tgtaactata gagtcagaca agagacttta tgatgctgta gaattactag gaggaacgac
     2281 aaaagaagca gatttaaatg gagtcaatct atcagtaaga ttagaagatg aacaacatta
     2341 cataataccc aaaataggtg aagcaacatc tgttacaagt aatgatgatt ccaataaacc
     2401 aaatcagagg aatgagtcaa gaggtgagcc taaaaataaa tccaataaaa atatatctaa
     2461 cgaatcaaaa gtaaatataa atgtagcgac tatagaagag ttggattctc tacctggggt
     2521 tggagaagct acagccaata aaattttaca acacagagaa gaaaatggac agtttagttc
     2581 aattgaggaa ataaaaaatg taaatggtat tggagataaa aagtatgaaa atataaaaga
     2641 tttaatttgt gtagactaaa atataataca aaacttacat atagatatgt tattataaat
     2701 atgtatgtat atgtgtgtac atgtggaaat ttagtaaagg aggcactttt atgaaaaaag
     2761 aacaaaaaaa acttacagag atgacaacag caggtggttg agccgctaaa atagggccag
     2821 aggttctggc atcagtttta tctcaattgc ctaaaaatga taatatagaa aatttattag
     2881 taggactaga tacagcagat gatgctgcag tttataaatt aaatgatgat atggcactta
     2941 tccaaacttt agacttcttt acaccaatgg tagatgaccc atacgtattt ggtcaaattg
     3001 ctgcatcaaa ttcactatca gatgtatatg ctatgggagg caaacctttg gttgctatga
     3061 atattgtttg ttttccatca tgccatgata tggatgtact tgcagaaata ttaaaaggtg
     3121 ggtttgataa agtgaaagaa agtggagcac ttttggttgg agggcatact gtagatgaca
     3181 aagagcctaa atatggtctt tcagtttctg ggattgtaag tccaaataaa gttttatcaa
     3241 atgctactgc aaaacctggt gataagttaa ttataacaaa accaattgga gtgggtgtat
     3301 taaatacagc catgaaagaa ggtatggtag aacaacatat agcagataaa gtaatagaaa
     3361 taatgataca tttaaataaa tatgcagcaa tgagttttga gaaatttgat gtaaattcgg
     3421 ttacagatat aactggtttt ggtctacttg gtcatacttt agaaatggcc aaagcttctg
     3481 aagtaagtat tgaaattgaa tcaaaacatg taccaattat tgaaggtgca atagaaatgg
     3541 cacaaatggg aattatacct gctggtatgt ataaaaatat gcattatgta tctaaagatg
     3601 tagaagttgt aggaaatata gaagttgcag ttcaagatat tttatacgac ccacaaacat
     3661 caggagggtt gttaatttca gtaaaagaag aacttgcaga ggaacttgta aaagatatga
     3721 aattaaatgg agcaatagaa gctaaaatta taggttctgt agttccaaaa ggggaaaaat
     3781 atattacagt tttataaaat attatagaga aagagtgatt gttttgagca aaagagaatt
     3841 gtttgcaatg ttgccttcag ttgatgaagt attaagtgat aatagaattg tagaaataat
     3901 aaatgaatat cctagaagtt tagttttaga atctgtgaga gaagtaatag atttaaaaag
     3961 acaatttata ttaagattaa aagaagatgc atcaaactct gtaacaatag agtttggtga
     4021 aataatagaa tctgcaatag agagagttaa gttaaattac tcattatctt taaaaaaagt
     4081 tataaatgca actggtacag ttattcatac aaatttagga aggtctttac ttagtgaaga
     4141 tataaaagat gaattatggt gtgctgcttc aaggtattca aacttagaat acgatttgga
     4201 taatggagaa agaggttcaa gatactctca tttaactagt acaataaaaa gacttactgg
     4261 agcagaagat gtactagttg taaataataa tgcagcggca gtacttcttg tacttagcac
     4321 aatggctaaa ggaggagaag caattgtatc tcgtggagaa ttagttgaag taggtggttc
     4381 atttaggatt ccaagtataa tggctcttag tggtgcagaa ttagttgaag ttggttcaac
     4441 taataaaact catttgaaag attacaagga ggccattaca gaagatacaa atgtattgat
     4501 gaaggttcat acaagtaatt atagaataat gggattcaca gagagtgttt ctatagaaga
     4561 attggttaat ttaggtaaaa aatataaact acctgtaata gaagacttag gaagtggagt
     4621 ttttatagat ttatcaaaat acggtttatc ttatgaacca actgtattgg attctattcg
     4681 acaaggtgca gatgtagtaa cttttagtgg agataagatg cttggaggtc cacaagcagg
     4741 tattatagtt gggaaaaaag agtatataga aaaaatgaag aaaaatcaac ttactagagc
     4801 acttagagtt gataagctta caatatgtgc tcttgaagca acattgagaa tgtatttaga
     4861 tgagacaaaa gctatagaaa atatacctac tttaaaaatg ttgacatata aaattgaaga
     4921 attagaagta aaagcaaata aactatttga aaaaataact gctttaaact taaatgcaaa
     4981 cataaatata gaagatggat tttcacaagt gggaggaggg tctatgcctc ttgaaactat
     5041 aagtacaaag gtaatatcaa taactcctga acatatgaat gtttcatctt tagaaaaaaa
     5101 actaagactt agtgaagcac atataatagc cagagtgtat gataataaat atgtactaga
     5161 tgtaagaact atatttgatg atgaatttga tgtaatagtt gcggaattga gaaaagcatt
     5221 taattaaata aatttgagtt aataaatgtt aaatttagaa gtataagtat gaagttttaa
     5281 ttaaaaaata gctacacaca cttgtgactt tagtcatagc aggtttagag ggggcaaaaa
     5341 atgaaaaatg tagtaattgg aacagcaggt catattgacc acggaaaaac tactcttata
     5401 aaagcattga ctggaagaga gactgatacg ttagatgaag aaaaaaagag aggtatttca
     5461 ataaacttag gatttactta ttttgattta ccaaacaata gaagagctgg aattgttgat
     5521 gtcccaggac acgaaaaatt tataaaaaac atgttagctg gagcatcagg aattgatata
     5581 gttttacttg ttgtagcagc agatgaaggg gttatgccac agacagttga gcatttagat
     5641 attctaagct ttttaaatat caaaaatgga atagtggttc taactaagtc tgatactgta
     5701 gatgatgaat ttagagagct agtaaaagaa gatatttgtg aaaaaataaa aggaactttt
     5761 cttgaagatg ctgaaataat tgaagttgat tctatatcta aaagaggtat agacactctt
     5821 attgaaaaaa tagataaaat gagtgatgat atagaagata aaaatgaaaa ctctccagca
     5881 agacttaata tagacagagt attttcaata aaagggtttg gtactgtaat aacaggcact
     5941 ttaatagaag gaaagataag tatagaagat gatttggtta tatatccaca ggtattaaaa
     6001 acaaaaataa gaagtataca agttcatgga gaaaataaag atacagctta tgcaggtcaa
     6061 agaacagcaa tcaatatatc aaatgtaaaa gtggatgata taaaaagagg agatgtttta
     6121 gctgcaccaa actcattgga ggaatcaatg atgttagatg taaaattatc attggtaaat
     6181 catttagata aaggattaaa gcactgggat agattaagat tgtatcatgg aactagagag
     6241 atactttgta gagcagttcc actagataaa gaattaattg aaagtggaga aagtggctat
     6301 gttcagttga gacttgaaga aagcatagta tctaagaaag gtgatacgtt tgtagtgaga
     6361 cgttattctc caatggaaac tataggtgga ggtgtaataa ttgacccatc tcctaaaaaa
     6421 cataagaaat ttgatgaaaa agttatagaa gcattaaaaa taaaagaaaa aggcgaacta
     6481 aaagatataa tagaagagta cttaaaaagg aatttaaaaa attatccaaa tataaaagaa
     6541 ataatgagct atagtggtgc tcatgaagaa gatgtaaaaa gagcgcttga aacacttata
     6601 tcagaagata aagtatttat tataggaaat atgtatatgc atataaatca atacaataaa
     6661 ttaaaagaaa atacaataaa gcttttaagt gagtaccata agaagtatag gcttagaaaa
     6721 gggatactaa aagaagaagt aaggtctaag atagagagta attttaagac tagggagatg
     6781 gatattttat tagaaaaatt gtctacagaa aatgctataa aaatagaaaa taatatagta
     6841 tctctattag attttgaggt tattttaaat gataagcaaa aagaaatagc aaaaaaaata
     6901 gaaaaaagat taaaatcatg tggagtttct tctatactga caatagatga agtttctgaa
     6961 ggaaatcata actatgctga agtattggag tcaatgatag gtaataaagt agagaaatta
     7021 gatgatttgt atataatgga caaagatatt tatgaaaatg caaagaacat tttgataaat
     7081 tatataaaag aaaataaaga aataacatta ggagaataca gagatttgat tgattctagc
     7141 agaaagaatt gcatgattat attggaaaac ttcgatagaa acaaaataac taaaagagta
     7201 gaaaacaaaa gaattttgtt ttagcagaaa atgtctgttt ttgctttaag gtataaagaa
     7261 ggaattataa tgtcgagtat cgtataatta attagtataa aatattaatt aagaggaagt
     7321 gtaaagtatt gaagaatata atacctgatg taaaaccaga tataaaagaa agagaagaca
     7381 tgagcctttt atttataata atgggctcag tacttttatt atttattata aaagatatag
     7441 agaaatatga aactttatta catgccatat atacgatatt tttagtagct acaggagtaa
     7501 ttatatttaa tactacaaaa tttaaagtag attcattttc tacattttta ggattaattt
     7561 ttatagctac tggagtattg gagtgtatat atttatttaa ttccttgggg attaaaacta
     7621 agtctataat ggagattaac atcacaatat cagcaataac agatttattt cctatactgg
     7681 gtgtatactt aagttttaaa tttgttaagg ataacaaaca aatttattca agtgtaattt
     7741 tatttatagc aactgctaca ttaacaataa catctttatt cataatatca ggaatttgtg
     7801 actatctggg aaatggtaaa tcacaatatg ctttaggagt tataatatct atattcataa
     7861 taattatttc tatcgttagt ggaattgaat taaattcaag ttcaaataaa ggtaaatggg
     7921 aatatgatga aaaaaagatt tttaacagaa taatagtaat aataatctta tcaagagttc
     7981 caaatctatt gcatatagtt attgataata gaaatgtaga gaggatatta tcacaaataa
     8041 taataaatgt agccttatat tatttatata attacatagt atctaagaat attaagaaaa
     8101 cagcactgga attgcatgat acgaatgaag aattaacaaa aaaaacggaa agtttaaaag
     8161 aaaagaataa aaaattaatc tatgagacag aaaagataga ggaacttaag ggaatactta
     8221 tgaaaagaga atctaggcta caatctacat tagatgtagc agtaaatagc atagtagtat
     8281 ttagcaaaga tggagaaatt acatatgcaa ataagagctt tagaaatact tttggagact
     8341 ataaagaaca agataaattg actgataaaa taaaaaactt caatgatttg atagaaagca
     8401 ttcattatgt gtttgttcat gagaagaatg cagagaaact tattagtaca agcgataata
     8461 aagtttatca agctatattt gcacctttaa ttattgcttc acaaacagaa ggagtattgt
     8521 gtgtactaga agataagact aagaaaaagg agtacgaacg caaacttatt gaagcaaata
     8581 agagaagtaa agatttttta gaaagtattg gagatggcat agtagtgctt gaaggtaata
     8641 acaaaatata tgttaataat gcatgtaggg aaatttttaa aaataattta gagagtattg
     8701 atttttcatt agtatgtaaa agtgaagaaa gtatagaaaa aagatatgta atagatggtg
     8761 aagtaaagta tgtagagatg agtttttctc agtatactaa tgctggtact aataaaacta
     8821 ttattgtaat acgtgataca acctctcgaa aaatatctca aataaaactt gaggaaagtc
     8881 aatcatcata ttcaagattt atagatatat tacctgatgg catatgctta ttaaaagaag
     8941 atttaagtat aaactatgca aataagtcgt ttttagatat gctagctttt acaaacatag
     9001 atgatataaa agattctaat attaaactat taatgaatgc gaatactgaa gaaaaaatga
     9061 aatttacgga taatatggct aaagttttaa atgaaaatag aagtatactt ttactggaat
     9121 atgaattgat aaatagttat gatgatatag tagaagtaga attaagtgca ttaccttttg
     9181 ctatatacaa tactagatac ataatgctta ttataaaaga tttagtacat aagaaatatt
     9241 ccgagcaagc agaaaaagaa ctgcttgaaa gatttaaaac agataaaatc aaaacggagt
     9301 tttttgcaaa tatgtctcat gaactaaaaa ctccactaaa tgttatatct agttcaaatc
     9361 aactggtaga ttctttctat agaaatgaaa agataaaaga ttacaataac aatattaaat
     9421 ctcatgtgga tttagttaga caaagttctt ataggcttca aagattaata aataacatta
     9481 tagatttaac aaagatggag tctgggttct atacactaaa acttgcgaaa tacaatattg
     9541 tatctgtaat agaagactta tttatgaata tagaagagta tgcacttaga aaagacataa
     9601 aaattctttt tgatacagac cttgaagaaa ttaatgttta tatagacaag gttgaaattg
     9661 agagaattat gttgaaccta ttgtcaaact gtattaagtt tactgataat ggaggctgga
     9721 tatatgtaag tattcattat aaaatagata aagtaatcat aagtgtaaaa gacactggag
     9781 ttggaatacc acaagataaa ttagaattga tttttgaaga attttcacaa gttgataaga
     9841 ctttatccag aaatactgaa ggaagtggaa tagggcttgc tatagttaag aatctagtaa
     9901 gtcttcatgg tggagatata gaagtagtaa gtgaagtgaa taaaggcaca gagttcttaa
     9961 tttcacttcc aatgaaggga tttagtagtg aacattacac agaagataag agaatatata
    10021 atattcaaga aaaaataaaa atagaatttt ctgatatata ctattaaaaa tgattatgag
    10081 caaaaataaa ataaaaatac tatttgttta aatatttaag ttgtaagaaa taggctatca
    10141 taaggtaaat tattatgata gcctattttt agctaggttt tatatctata tgtgagaaca
    10201 tataatctct aaattacctt caaaactaaa atctccaaag tcgtttggca gtataaaatg
    10261 catacccttt tttaaaatat attctacaga attgtgtata agttttccat ttccatccaa
    10321 cacactgtat aaagaaaaag gtgtattgtg tgtaaaatta cattttccaa aaacatctaa
    10381 tttataaaca ctaaaaaatt cgtttgacac aaatgttgtg catttaaagt ctgatgttga
    10441 tacgatttta tagtcagtat cgaaatttat atgaggaaca ttcgtaacat ctattgattt
    10501 ttgaacatga agttcacgct tattacctga attgtcagtt ctatcataat catagactct
    10561 ataagtagta tctgagtttt gttgagtttc taaaatcaat gttcctttgc atatagcatg
    10621 tatagtacca cttggaacat agaaaaagtc tccctttttt atattaacct ttctaagtag
    10681 tttgtcccat tcattattat ttaccatatt tacaaattct ttatgagatt ttgcattatg
    10741 acctattata atttctgcat catcagaaca atctattaca taccaacact ctgtcttacc
    10801 taactctcca ttttcatttt ttttagcata ttcatcattt ggatgaactt gaactgataa
    10861 attatcattt gcatccaata tttttgtaag taaaggaaac ttatcaccag gtgtgtttcc
    10921 aaataattct ctatttttat tccacaattc agatagtttt ttacctttat attttccatt
    10981 ttctattaag caatctccat ttttatgaga gcttatagcc caacattcac cagttgttgg
    11041 cgaatctatt tcataattaa atttatcttt aagtgcagta ccaccccata ttctatccat
    11101 aaaaataggt tttaaaaata atggttccat aatcctgttc ctcctatgat attaaattta
    11161 tcctacaaat aattatatat aaatagatta taaaatcaat agtaaaaaag ttttgtccct
    11221 tgaaatgaca taaagtataa ggagttatta aataagataa actatgatat ataatataaa
    11281 gttctaaatt ggagtgataa ttatggagtt gtttaaggca gaaagcaaaa aatataaaaa
    11341 agaattttta aaattattgg agcaatgggt tagtatacct tcattttatg atagaaaaac
    11401 tgtatctaaa gatatgcctt ttggaaaagg agtatatgat gcattaaact ggtttgaaaa
    11461 tctaggtaga gaaaacaatt tcaaagtaaa aaatatagat aatcatgctg ttcaaattga
    11521 atatggaaat ggtaaagagt atgtagatat atttggacat tgtgatgttg taaatccagg
    11581 tgaaggatgg gatagtgaac catttaaact aaatattata ggtgataaat tagttgctag
    11641 aggtgtatct gataataaag ggcctatgat agtgaatttt ttagcactaa aaatgataaa
    11701 ggatttagat attaatttaa aaagaaaggt tagattaata gctgggggaa atgaagagag
    11761 tggatttaaa tgtataaagc attattatag caaagagcca tatggtgtat gtggatttac
    11821 tccagatgct aaatttcctg tattaaatgg agaaaaaggt ggagcaataa taaagttaat
    11881 ttcaaatata gacgataaat ctttatatat aagtggtgga atagagttta atacaatacc
    11941 agataaagtt tatataaaga atgtagaaaa attgggtaaa gataatatat gttttgatat
    12001 taacaatata tcgataaatt atgataatgg caattatatt gtgtatggaa aaggtggtca
    12061 ttcatctaaa cccgaaaaat caataaatcc tatattagct acaattaaac ttttgtcaga
    12121 aaatattgat gaaaaatgga caaaagattt atataagttg ataaatcaag ataatataaa
    12181 tggaaactta tttggtttaa atattgaggg gaaatgtggg attttatcaa tggtgccaac
    12241 aataataaat atagtagatg gaaaattaga agtagtttta agcgttagat atccagaaat
    12301 attaaccatt gaagatataa taaaaaaatt taatttgtat atggagcaaa ataatattaa
    12361 taaatttgag cttattgggg aaaatttaaa gcaagcaaat tatatagata gaaattcaaa
    12421 acttgtaaga agtttgcatg atatatatat aaagtattct ggagatttaa aaaatgatgt
    12481 tagagtaact agtgcaggaa gttatgcttc tgaaatgaat aattcagtaa tatttggttg
    12541 tgaatttcct gatggtagct ttggcaatgt ccatagtgcg aatgaatttg ctagcttaga
    12601 tagatttata actgcaatag gtatatatgc agaggcaata attacattat gtaataaaat
    12661 ataaatacaa aatgtccctt atatagacat attatatata caattaaaat tttacttgat
    12721 atataattga aatcaaagag aaggtgatac aatgataact aagaaacatg caataatagt
    12781 aaagagttta agcaataaag atggatatat gacatcaaac gaattagcta tcaaattgga
    12841 tgtgtctact aaaactatta aaagatatat agcagattta aatagtgttt taagcaagta
    12901 tgatttggta atagcttctt ctagaggtat aggatacaag ttaagcggtt ctaaaaacaa
    12961 tatagccaga gctgttaaag aagcaaataa atatattgat ggttttttag atgactctga
    13021 agagtcaaga atgtcaaata taatatgtat gcttattaat agaaattata tgagtataga
    13081 agcaatggca gaagaattaa atctaagtat agccgctata aataaattgt ctagtaagct
    13141 taaaagaaaa cttgaaaagt atgacttagt tataaaatct aaaccttact atggaagcta
    13201 tatacatggt gaagaaatta atataaggca attaattact gactatgcaa taaaattaga
    13261 tgaaaaaagt aaagtgaagg tgtttttaga tgacattagt gaaaacgata tacactgtat
    13321 agaaagtatt ttagaaaagc acttaagaga aaaagataca ataatttcag ataaagattt
    13381 taatcttttg ctttcaaaaa taatcgtttc tgtatttagg agtaagagag gtcatagtaa
    13441 caatataaac ttaatggata caagttatag atttcataac tactacttta tagaaaatct
    13501 aatgaaagaa atatctgata aaattggatt taaacttatt gaagatgaag taatttatat
    13561 ttcaaattat tctggtgtaa tagcctataa aggtacacaa ggaagaaaaa acacaagtga
    13621 aattgaggaa cgaataagta gtgttataag tagtgcactt caagatatat tccttatttc
    13681 aggtagtgat tatacaaaag atgatgagtt tatggttgca atctctgacc atataaagag
    13741 atttttaaat agagctagag ctaatgtaaa atcaaataat cctttacttc atcaaataaa
    13801 agagaagttt cctatagctt ttaatttagc cgtgttttta tcaaataaac tagaaactga
    13861 gtttaattta aaacttgatg aagatgagct tggatatata gctatccact ttgctgcttc
    13921 aaatgaaaga atgaaaaaaa atactagtaa gaaaatttgt atagtttgtc attatgggat
    13981 aggaacagga caacttattt ctgaaaagct aaaacagaat ataagtgatt taagtgttgt
    14041 gggtgtttat ccagttagat atctcgatat ggctataagt caagatgttg atttgatagt
    14101 atctaccgtt gaattaaagg gatatgaaaa acctgtctta tatatagaaa atatatttga
    14161 tgatagctta attgaaaacg taaacaaagc cttttatgaa aaggaagaaa gaagaaagat
    14221 aataagcaac atgtttgatg aaaaagcttt tttcagtata aaggctaata ctaaagaaga
    14281 ggtcataatt tcactttcaa ataaactaaa agaaagagat tttatagagg aaagttctat
    14341 aaaaagtata atagatagag agaatatttc atcaactgaa ataggaaatc tagtagcaat
    14401 accccacaca atagtgaagg gagataaaaa atctataata ggagttggaa tacttgaaaa
    14461 tccaataatt tgggataagc aagaggttca gctagtattt atggtatttt ttaatactaa
    14521 agaaaaacat aatttctcga tatttaaata cctttataat tttataaaag atgaaggcgg
    14581 agtaagagga attattaaaa tatgtgactt taataaatta atggcactga taggtaatta
    14641 aggaggaaat taataatggg aacaaacata tttaacaaag aatatgtatt tttaaatgtt
    14701 gatgctaagt caaaggttga agttttaaaa tttatatcaa aaaaagctaa agacttaaac
    14761 ttggcagaag atgagagctt agtatatgaa ggtttaatgg ctagagaaga acaatttact
    14821 acaaatttag gagaatctat agcaatacct cacactaaaa atgatgcaat tgaaaatcca
    14881 gcagtagtag tattgaagtt taatgaagat gttgtttgga atgaaggtga ggataaggtt
    14941 aaattagcaa taagcttact tatgccagga aaaagtaaag agaatattca tcttaaatta
    15001 ttatcatctc tttcaagaaa attaataaat aaagaattta aagattctct tttaaaaagt
    15061 gataatgttg aagaaatatc taacttaata aatgaagctt taggtttata attaaacact
    15121 aaggttataa aataaaaaaa taattaacta aaaataggag gaatttgaaa tgaataaaaa
    15181 attagtagca ttatgtgctt gtccaatggg actagctcac actttcatgg cagcagaagc
    15241 aatagagcaa gcagcaaagg cattaggtta tgaagcaaaa gttgaaactc aaggtgctga
    15301 tggtgtacaa aatgagttaa caagagatga tatattagga gcaactatga taatacatgc
    15361 agtagcaata acaccagaag gtatggaaag atttgatggt tgtgaagttt atgaagttga
    15421 attacaagaa gctataaaaa atgcagaagg tgttataaaa gaaatagaag aagatttagg
    15481 aatataataa atttattgtg gaggaaatag aatggcaatt aagaaaaaag ttataaacag
    15541 ttcaggtgct tctacaacag gaggaaataa tgtaggaaaa tctactccat ctagtccaac
    15601 tcaatcaaag ggaaatggaa aatggaaaga aataagtaaa catataatga caggtatatc
    15661 atatatgata ccagttttag taatgggtgg acttataggg gccttatctc aattaatacc
    15721 atatgctata ttaggacttg acccatctgt aggtatagtt gatgctatga actcaggaga
    15781 atttacaggg ttcaagctta gtcttttaaa tatagcacaa ttaatgtcaa atttcggatt
    15841 tactttgttt ggatttgcga taccactgtt tgcagcattt tgtgcaaact ctataggtgg
    15901 aaaaacagct ttaattgcag gatttatagg tggatatata gctaataaac ctgtaggtgt
    15961 accacaattc gttgatggac aatggactga agttgttcca gttgcatcag gatttttagg
    16021 tgcaatacta atagcattta taataggata ttttgtaaaa tggttaaata aatctataaa
    16081 agtttctcat aactggttag cttttaaaac aacattttta ataccattaa tagcgtcatt
    16141 agcttgtatg gtattgatga tatttataat aactccattt ggtggattaa ttaatgaaag
    16201 tatgaaaaac ttcttaacag cagcaggagc agctggagaa tatgtatatg caacagcttt
    16261 ggcagcagct acagcattcg acttaggagg accaataaat aaagcagcag gattcgttgc
    16321 acttggttta actacagaga atgtacttcc aataacagca agaacaatag ctatagttgt
    16381 tcctccaatt ggattaggat taacaacatt attagataaa agattagtag gaagaagagt
    16441 atatgataga caattctatc aagcaggaaa aacttctata ttcttatcat ttatgggtat
    16501 atctgaagga gcaataccat ttgcacttga gagaccaggt tttgttatac ctttaaacat
    16561 agttggaagt gtaataggag ctattacagg tataattcta ggagcaattc aatggttccc
    16621 agagtcagct atatgggcat ggcctctagt agataactta tttggatata taataggtat
    16681 agcagttggt gctatattca tagcagtagg aaatatattc tatagaaata aattaataaa
    16741 agatggtaaa cttgttgtag attatatcga ctagtattta atttttacta tctataaata
    16801 actttggctg gtaatatggt tttaatcaat attaccagcc ttacctatat gattaattaa
    16861 aggcggtgga gataagatat ggataagata gaaaaagtaa gagagtattt tagagaatat
    16921 gatatagatg gatttcttat aaatagtcct acaaacaagt tttatgttgg aaatttattt
    16981 agctcttcag gttatgtttt tataacgaaa gaatcacaat atataatagt agattttaga
    17041 tattttgaag aaattaaaag aaaatctagt ttgtttaatg tagtattaat ggacaaaact
    17101 agaactcatt ttgatataat taatgatatt tgtagagagc agaatataaa ggaaattgga
    17161 tttgagggaa atgaagtatc ttttgattta tatagaagta tgagtaataa gttaagtgca
    17221 accttaaaat cagttgattt atctacatta agggaaacaa aaaatgaaga cgaaataaaa
    17281 tatataaaaa aagcttgtga aatagttgat gctacatttt atcatatagt agactttata
    17341 aaggttggta tgactgaaaa gcaagttgaa aatgaaattg ttagaattat aaaagattta
    17401 ggtggtcaaa aagaatcttt cgatacaata gttgcatctg ggcttagagg agcattacct
    17461 catggaaaag caagtgaaaa agttatagag tatggagatt ttgttacttt tgactttggt
    17521 gctaagtata ataattattg ttcagatatt accagaacca tttgtatggg tactataaat
    17581 aaagagttag aagaaatata taatatagta agaaaagcta atgaagagtg tataagggtt
    17641 ttaagaccgg gaatgactac tggagagata gataaagttg caagagacat aattggttca
    17701 tatggatatg ccaataattt tggtcataat ctgggtcatg gtgtaggaat tatggttcat
    17761 gaatatcctg cattagctcc tgaatcaaat gaagtattaa aagaaggaat gattgtaact
    17821 atagagcctg gtatatatgt tccaagtctt ggaggcgtaa gaatagaaga tgatgtactt
    17881 ataacacaag atggatgtat gagattgact acatctacta aggatttaat agttgtaagc
    17941 taaaaataaa ttagataaat attttataac aataagaaaa gaagaggtaa atttaaatgt
    18001 ataattttga tgataaacca gatagagtaa gtgaaaagtg tagaaaatgg gatttaaata
    18061 ttataagaga taaatttgga gatattagag aagattttgt accaatgtgg attgctgata
    18121 tggattttaa aataccaaca gaaatagaaa acaaatttat tgaagctgta agaagaggtg
    18181 tatttggata tacatactgt tatgatgaat tttatgattc agttattggt tggcaaaaaa
    18241 atatgcatga ggtaaatgta gaaaaagaat ggattacatt aacttatgga actgtatcaa
    18301 cacttcatta tgtagtacaa gctttttgta agaaaggtga tagtataata ttaaacacac
    18361 cagtttatga cccctttgaa tcatcagcaa aaaaacaagg tgtaaatgta atttgtaata
    18421 cattagatgt tgtaaatgat agatattata taaattttga taaattagaa gttcaaatta
    18481 aagaaaataa gcctaaatta atgatgtttt gtacaccaca taatccatct ggaaggatat
    18541 ggacaattga agaaatgaca agggtagcaa ctatatgtaa agaaaataat gttattttgg
    18601 ttgcagatga agtacatgca gagcatattc attatggtac atttaattca atacttaaga
    18661 ttggaaaaga gcttttagaa aatgttattc tattgacatc accaaataaa ggatttaatc
    18721 ttggtggatt aaagacttct tattcaatag ttataaataa ggatataagg gacaaattta
    18781 gaaataaatt aaagcaaaat tctataactt ctccaaatgt gtttggaata ataggattga
    18841 taaccgctta taatgaatgt catgagtggt taagtggtgt aaatgaatat ataaaatcaa
    18901 attatgaact tttagaaact tgggtaaata aatataataa gataaaattg atgaaaatgg
    18961 aatcatctta cttagcttgg atggatataa gtggattagg tattagtgca agtgagttta
    19021 cagataaatt ggctatagat acaggagtat tattagaaga tgggagtcat tttgtaaaag
    19081 atggtgagaa gtatgttaga ataaatttag gaactcaaaa ggaaaatgta attgaagctt
    19141 taaatagaat ggatttattt ttaaagtctt tataaaaatt tggttgaatt agtattttat
    19201 ataaatttaa actattaata tggtatatac ataagtagaa tttgttttag attacttaag
    19261 tatataccat attttatata aaaattaaat ttgcattagt catatgataa ctatttaaat
    19321 tcatatgtat tttgtatgta taatagatat aattattatt taaacttatt gtttatttaa
    19381 tttttcaata gaaaaagatt tatctgtaag tttaaaatta tcttttatac ccttagttag
    19441 gtatactttc ttatccttag ttatgaatat agcatctaca ttatcaattt tattaattaa
    19501 atccatacct ttttcaagac caagtccaaa tgtagaagtg gataaggcat cacaattaat
    19561 agatttatca gagattattg taacactact taaattgttt tcaaaaggat accctgtaaa
    19621 tggatttaac atatgatgat atatttttcc gtttttttcg ataaatcttt cataaattcc
    19681 agaagtaaca acagatttat ttgtggtttc tatgtttcct atggattcac catttttact
    19741 agttgggtct tgaatgccta ttttaaaagg tttatcattt ttaccatctt ctaaagtaaa
    19801 tatatttcct cctaagttta ctaaaccttt ttttacatcc tctgatttta aataggctac
    19861 aattttatca gcagcatatc ctttagctat ggcacctaaa tcaattttca tattttcttt
    19921 ggttaatttt atagatttat ttttttcatc caatatgatg ttcttataat caataagagg
    19981 cagaagtttt tgtatctcag aatcacttgg aactttagct ttatctgttc caatagccca
    20041 taaatttgat aatggaccaa ttgttatgtc aaaatatcca tctgataatt tggaatattg
    20101 tatagcttct tttacgacga aaaatgtatc atcagaaact ctgacaaatt ttttaccagc
    20161 attatcattt attttagaaa catcacttcc tggtatatga gtgctcattt tgttgtcaat
    20221 atatcttaaa attgaatcac attcatttaa gattttatca cttttggatt cttttacatt
    20281 aaatacagta acttcgttta ctgtaccaag gtagtagcta gttttactat atttagtatt
    20341 ctggcttgaa ctcctaaaaa atgaaaataa aattaatgct aaaatcaata tcacaaaaat
    20401 aaatgtgatt tttttcttat tcatggtata ttcctcctat gaaattgtgt tattatttta
    20461 ttataacata atttcatagg aggacaaaaa atgaagaaaa aagacattct tcttatttta
    20521 ggcattttgg ttgttattac agcatgttat gggataataa atgttataaa ttctaaaaat
    20581 gctggaaata ttgagattta tgtagataat aaattgtata aaactgtttc aataaatgcc
    20641 aaagaagaat ttaaaataga gaatagaggg gggtataaca tagtaaagat tcatgataaa
    20701 ggggttgaga tagtagatgc ttcatgccct gataaggttt gcgtacatac tggatttata
    20761 aacaagccaa gtcaaagcat agtttgtata cctaatagag tgagcataaa aataaataca
    20821 aatgaaaaga atgataatca agaagatata atttctaaat aaaataataa aaataattga
    20881 aaggaattgg tgataattat ggttaactta ggaaacgatt gggatgaatt attgaaagaa
    20941 gaatttgaaa aagattacta cttaaactta agaaaatttc ttatagaaga atataaaaca
    21001 cgccaaattt tcccaaatat gcacaatata tatgaagctt taaaacacac ttcatacaaa
    21061 gacacaaaag tcttaatatt aggacaagac ccttatcatg gagataatca agctcatggt
    21121 ttagcttttt cagttcaacc acaagtgaaa actccaccat ctttattaaa tatgtataaa
    21181 gaattaaaag atgatttagg ttgttttata cctaataatg gatatttaat gccttgggca
    21241 gaccaaggag tgttactttt aaatactgct cttactgtta gagcgcatga agctaattct
    21301 cataagaaca aaggatggga aattttcact gatagagtaa tatctatttt aagtgagcgt
    21361 gaagacccag ttatttttgt actatgggga agtaatgcac gtaaaaaagt agagttgatt
    21421 gatacaagta aacattatat tttggaagca cctcatccaa gcccattgtc agcaagtaaa
    21481 ggcttttttg gatgtaaaca cttctctaag ataaatgaaa ttttgaaaaa gctaggaaaa
    21541 gaacctatta attggcagat agaaaatata taaaagtaaa aggtaacaaa atgataacaa
    21601 aacaacaata aacaatgaca ttgttgtaac taatagaaat atgcttaaat agttgaaaat
    21661 actatgtatt catatttcta tttttttgtc aagttgacaa ctcttgaaaa aatagttaca
    21721 atttagttac acaaaaaaca caaataaaaa aagaaactta caaagcagaa ttttatgaag
    21781 caaaatatat agtgtaaaaa atcttaggga ggaaatttta tatgaatttt aaaaaattga
    21841 gtaatgctaa agtctcggta atggcagttg ttttatcagt tggaatatta tcgggatact
    21901 ctgcgcttaa taaacaagta acattagtag taaggggaga agaaagaact atatcaacat
    21961 ttagttctaa tgtaggagac cttttagaat ctcaaaacat tagctatgat tcaaatgata
    22021 tagtaacttt agatgaaaat acaaagttat ctaatggaga taaaatagaa gtaattgatg
    22081 taaaggaaaa aacagttgta gaaactaaag acattccatt tgaagttaca gttgttgaag
    22141 atggctcttt attaaagggt gattctaagg ttaaagaaga aggaaagtct ggtaaaagtg
    22201 aattaatata taaagaaact tatcacaatg gaaagaaagt agaaaaggaa tttgtgaaaa
    22261 aggttgttaa agctgagcca gtaaataagg ttgtaagcaa agggacaaag gtagaaatgc
    22321 aagtagcctc atcaagggga gaaagttcaa gaagaatggc ttcatcatca tctagtagca
    22381 gtagtagtaa caatggtaaa aatatgagag tggtagcaac tgcatatact ggtcacagta
    22441 taactgcaac aggaacaaaa cctaagtggg gaacaatagc tgtagaccct aaggttatac
    22501 catatggaac taaagtttat atacctcaat ttgacatggt gtttactgca gaagactgtg
    22561 gtggagcaat aaaaggaaat aaaattgata tctacatgaa tgattcaaaa acagtttata
    22621 actggggaag aaaaactata gatatacgta tattaaaata gttaaataat ttatattttt
    22681 actggaaatt atattttgat tatatagaga agaaactctt tatagattct aaaaatataa
    22741 tttccagttt tttattttgg atatgtaata aaacaggtaa attttaacca tgtatagata
    22801 aaagctattt aaattataat ttactatcat ataaatctat aaaaattgtt gcaaagagtc
    22861 aaaatatatg atattataga tttcgttgag aaggatttca ttatgggagt ggatgggtgc
    22921 cggtgtgccc tctagtcttc aaaactagta tgaggggtta agagcctctt aggtgggttc
    22981 gattcccacg cattcccgcc aatatgtaaa aggtatcttt taattaaaag atacc
//
LOCUS       DAELJO010000044        22938 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELJO010000044 DAELJO010000000
VERSION     DAELJO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.044"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            333..1469
                     /locus_tag="KPB88_001475"
     CDS             333..1469
                     /locus_tag="KPB88_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG0053764.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            1528..2310
                     /locus_tag="KPB88_001476"
     CDS             1528..2310
                     /locus_tag="KPB88_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0053765.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            2329..3339
                     /locus_tag="KPB88_001477"
     CDS             2329..3339
                     /locus_tag="KPB88_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0053766.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            3358..4155
                     /locus_tag="KPB88_001478"
     CDS             3358..4155
                     /locus_tag="KPB88_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0053767.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            4253..5098
                     /locus_tag="KPB88_001479"
     CDS             4253..5098
                     /locus_tag="KPB88_001479"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG0053768.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            5185..6360
                     /locus_tag="KPB88_001480"
     CDS             5185..6360
                     /locus_tag="KPB88_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0053769.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            6555..7457
                     /locus_tag="KPB88_001481"
     CDS             6555..7457
                     /locus_tag="KPB88_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0053770.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            7471..8475
                     /locus_tag="KPB88_001482"
     CDS             7471..8475
                     /locus_tag="KPB88_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0053771.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(8619..8846)
                     /gene="acpP"
                     /locus_tag="KPB88_001483"
     CDS             complement(8619..8846)
                     /gene="acpP"
                     /locus_tag="KPB88_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0053772.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            9018..9443
                     /locus_tag="KPB88_001484"
     CDS             9018..9443
                     /locus_tag="KPB88_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053773.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            9491..9697
                     /locus_tag="KPB88_001485"
     CDS             9491..9697
                     /locus_tag="KPB88_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053774.1"
                     /translation="MTKLEDNMERFFGGCEGFFWNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            9879..10058
                     /locus_tag="KPB88_001486"
     CDS             9879..10058
                     /locus_tag="KPB88_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053775.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            10092..10292
                     /locus_tag="KPB88_001487"
     CDS             10092..10292
                     /locus_tag="KPB88_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053776.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            10545..11561
                     /gene="ccpA"
                     /locus_tag="KPB88_001488"
     CDS             10545..11561
                     /gene="ccpA"
                     /locus_tag="KPB88_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG0053777.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(11684..12082)
                     /locus_tag="KPB88_001489"
     CDS             complement(11684..12082)
                     /locus_tag="KPB88_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053778.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            12294..13205
                     /locus_tag="KPB88_001490"
     CDS             12294..13205
                     /locus_tag="KPB88_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053779.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            13320..13500
                     /gene="ssrS"
                     /locus_tag="KPB88_001491"
     ncRNA           13320..13500
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPB88_001491"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13683..14900
                     /gene="cdeC"
                     /locus_tag="KPB88_001492"
     CDS             13683..14900
                     /gene="cdeC"
                     /locus_tag="KPB88_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG0053780.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(15001..16323)
                     /locus_tag="KPB88_001493"
     CDS             complement(15001..16323)
                     /locus_tag="KPB88_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0053781.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(16374..17168)
                     /locus_tag="KPB88_001494"
     CDS             complement(16374..17168)
                     /locus_tag="KPB88_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0053782.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            17278..18723
                     /locus_tag="KPB88_001495"
     CDS             17278..18723
                     /locus_tag="KPB88_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0053783.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            18752..18961
                     /locus_tag="KPB88_001496"
     CDS             18752..18961
                     /locus_tag="KPB88_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG0053784.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            18981..20375
                     /locus_tag="KPB88_001497"
     CDS             18981..20375
                     /locus_tag="KPB88_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0053785.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            20615..20800
                     /locus_tag="KPB88_001498"
                     /pseudo
     CDS             20615..20800
                     /locus_tag="KPB88_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(20908..22074)
                     /locus_tag="KPB88_001499"
     CDS             complement(20908..22074)
                     /locus_tag="KPB88_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0053786.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(22212..22418)
                     /locus_tag="KPB88_001500"
     CDS             complement(22212..22418)
                     /locus_tag="KPB88_001500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053787.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(22477..22701)
                     /locus_tag="KPB88_001501"
                     /pseudo
     CDS             complement(22477..22701)
                     /locus_tag="KPB88_001501"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         8898 a         2628 c         4036 g         7376 t
ORIGIN      
        1 cagacaatat ttaatgttta atatacccta atataaatat cgtaaacaaa ttattttatc
       61 tgtaaccagt gtgaaggaaa tcttattcta acttgaaatt tcacaaaatt ccaaaaagcg
      121 agattttttt taagtatagt tgattttaaa ttaacaattt atgattaaat taatgtcata
      181 attggttagt ataatcacat ataaatatag agcgtagttt gattaaggtt ataaatttct
      241 cgacgtatat tcaaatacct tattatagat gttgccgaga ttttaattaa taaatgaaaa
      301 taaaatattt ataaaatact gaggaggcta ttatggattt aaattctaaa aaatatcaga
      361 tgcttaaaga gctatatgta agcttcgctg aaaatgaagt taaaccttta gcaacagaac
      421 ttgatgaaga agaaagattt ccttatgaaa cagtggaaaa aatggcaaaa gcaggaatga
      481 tgggtatacc gtatccaaaa gaatatggtg gagaaggtgg agacactgta ggatatataa
      541 tggcagttga agaattgtct agagtttgtg gtactacagg agttatatta tcagctcata
      601 catctcttgg ctcatggcct atatatcaat atggtaatga agaacaaaaa caaaaattct
      661 taagaccact agcaagtgga gaaaaattag gagcatttgg tcttactgag cctaatgctg
      721 gtacagatgc gtctggccaa caaacaactg ctgttttaga cggggatgaa tacatactta
      781 atggctcaaa aatatttata acaaacgcaa tagctggtga catatatgta gtaatggcaa
      841 tgactgataa atctaagggg aacaaaggaa tatcagcatt tatagttgaa aaaggaactc
      901 ctgggtttag ctttggagtt aaagaaaaga aaatgggtat aagaggttca gctacgagtg
      961 aattaatatt tgaggattgc agaataccta aagaaaattt acttggaaaa gaaggtcaag
     1021 gatttaagat agcaatgtct actcttgatg gtggtagaat tggtatagct gcacaagctt
     1081 taggtttagc acaaggtgct cttgatgaaa ctgttaaata tgtaaaagaa agagtacaat
     1141 ttggtagacc attatcaaaa ttccaaaata cacaattcca attagctgat atggaagtta
     1201 aggtacaagc ggctagacac cttgtatatc aagcagctat aaataaagac ttaggaaaac
     1261 cttatggagt agaagcagca atggcaaaat tatttgcagc tgaaacagct atggaagtta
     1321 ctacaaaagc tgtacaactt catggaggat atggatacac tcgtgactat ccagtagaaa
     1381 gaatgatgag agatgctaag ataactgaaa tatatgaagg aactagtgaa gttcaaagaa
     1441 tggttatttc aggaaaatta ttaaaataga taaatattaa aatatacaca ataggtcgat
     1501 attaaaatag atatggagga aggatttatg aatatagtcg tttgtataaa acaagttcca
     1561 gatacaacag aagttaaact agatcctaat acaggtactt taattagaga tggagtacca
     1621 agtataataa accctgatga taaagcaggt ttagaagaag ctataaaatt aaaagaagaa
     1681 atgggtgctc atgtaactgt tataacaatg ggacctcctc aagcagatat ggctttaaaa
     1741 gaagctttag caatgggtgc agatagaggt atattattaa cagatagagc atttgcaggt
     1801 gctgatactt gggcaacttc atcagcatta gcaggagcat taaaaaatat agattttgat
     1861 attataatag ctggaagaca ggcgatagat ggagatactg cacaagttgg acctcaaata
     1921 gctgaacatt taaatcttcc atcaataaca tatgctgaag aaataaaaac tgaaggtgaa
     1981 tatgtattag taaaaagaca atttgaagat tgttgccatg acttaaaagt taaaatgcca
     2041 tgccttataa caactcttaa agatatgaac acaccaagat acatgaaagt tggaagaata
     2101 tatgatgctt tcgaaaatga tgtagtagaa acatggactg taaaagatat agaagttgac
     2161 ccttctaatt taggtcttaa aggttctcca actagtgtat ttaaatcatt tacaaaatca
     2221 gttaaaccag ctggtacaat atacaatgaa gatgcgaaaa catcagctgg aattatcata
     2281 gataaattaa aagagaagta tatcatataa tttaaggggg gataaagaat gggtaacgtt
     2341 ttagtagtaa tagaacaaag agaaaatgta attcaaactg tttctttaga attactagga
     2401 aaggctacag aaatagcaaa agattatgat acaaaagttt ctgcattact tttaggtagt
     2461 aaggtagaag gtttaataga tacattagca cactatggtg cagatgaggt aatagtagta
     2521 gatgatgaag ctttagcagt gtatacaact gaaccatata caaaagcagc ttatgaagca
     2581 ataaaagcag ctgaccctat agttgtatta tttggtgcaa cttcaatagg tagagattta
     2641 gcgcctagag tttctgctag aatacataca ggtcttactg ctgactgtac aggtcttgca
     2701 gtagctgaag atacaaaatt attattaatg acaagacctg cctttggtgg aaatataatg
     2761 gcaacaatag tttgtaaaga tttcagacct caaatgtcta cagttagacc aggggttatg
     2821 aagaaaaatg aacctgatga aactaaagaa gctgtaatta accgtttcaa ggtagaattt
     2881 aatgatgctg ataaattagt tcaagttgta caagtaataa aagaagctaa aaaacaagtt
     2941 aaaatagaag atgctaagat attagtttct gctggacgtg gaatgggtgg aaaagaaaac
     3001 ttagacatac tttatgaatt agctgaaatt ataggtggag aagtttctgg ttctcgtgcc
     3061 actatagatg caggttggtt agataaagca agacaagttg gtcaaactgg taaaactgta
     3121 agaccagacc tttatatagc atgtggtata tctggagcaa tacaacatat agctggtatg
     3181 gaagatgctg agtttatagt tgctataaat aaaaatccag aagctccaat atttaaatat
     3241 gctgatgttg gtatagttgg agatgttcat aaagtgcttc cagaacttat cagtcagtta
     3301 agtgttgcaa aagaaaaagg tgaagtttta gctaactaat ttcttgggga gggtattatg
     3361 agtacaagtg atgttaaagt ttatgagaat gtagctgttg aagtagatgg aaatatatgt
     3421 acagtgaaaa tgaatagacc taaagccctt aatgcaataa attcaaagac tttagaagaa
     3481 ctttatgaag tatttgtaga tattaataat gatgaaacta ttgatgttgt aatattgaca
     3541 ggggaaggaa aggcatttgt agctggagca gatattgcat acatgaaaga tttagatgct
     3601 gtagctgcta aagattttag tatcttagga gcaaaagctt ttggagaaat agaaaatagt
     3661 aaaaaagtag tgatagctgc tgtaaacgga tttgctttag gtggaggatg tgaacttgca
     3721 atggcatgtg atataagaat tgcatctgct aaagctaaat ttggtcagcc agaagtaact
     3781 cttggaataa ctccaggata tggaggaact caaaggctta caagattggt tggaatggca
     3841 aaagcaaaag aattaatctt tacaggtcaa gttataaaag ctgatgaagc tgaaaaaata
     3901 gggctagtaa atagagttgt tgagccagac attttaatag aagaagttga gaaattagct
     3961 aagataatag ctaaaaatgc tcagcttgca gttagatact ctaaagaagc aatacaactt
     4021 ggtgctcaaa ctgatataaa tactggaata gatatagaat ctaatttatt tggtctttgt
     4081 ttttcaacta aagaccaaaa agaaggaatg tcagctttcg ttgaaaagag agaagctaac
     4141 tttataaaag ggtaaaaatc taattaaaaa tatatttgct gttaactaga ttaaatagta
     4201 ttttaatcta gttaacagaa tataaatact aaaataacag gaggttataa ctatgaaatt
     4261 agctgtaata ggtagtggaa ctatgggaag tggtattgta caaacttttg caagttgtgg
     4321 acatgatgta tgtttaaaga gtagaactca aggtgctata gataaatgtt tagctttatt
     4381 agataaaaat ttaactaagt tagttactaa gggaaaaatg gatgaagcta caaaagcaga
     4441 aatattaagt catgttagtt caactactaa ctatgaagat ttaaaagata tggatttaat
     4501 aatagaagca tctgtagaag acatgaatat aaagaaagat gttttcaagt tactagatga
     4561 attatgtaaa gaagatacta tcttggcaac aaatacttcg tcattatcta taacagaaat
     4621 agcttcttct actaagagac cagataaagt tataggaatg catttcttta atccagttcc
     4681 tatgatgaaa ttagttgaag ttataagtgg tcagttaaca tcaaaagtta cttttgatac
     4741 agtatttgaa ttatctaaga gtatcaataa agtaccagta gatgtatctg aatctcctgg
     4801 atttgtagta aatagaatac ttatacctat gataaatgaa gctgttggta tatatgcaga
     4861 tggtgttgca agtaaagaag aaatagatga agctatgaaa ttaggagcaa accatccaat
     4921 gggaccacta gcattaggtg atttaatcgg attagatgtt gttttagcta taatgaacgt
     4981 tttatatact gagtttggag atactaaata tagacctcat ccacttttag ctaaaatggt
     5041 tagagctaat caattaggaa gaaaaactaa gataggattc tatgattata ataaataaga
     5101 atttgggaat taaaagttta aataaaattg tttaaaaaac aatttcggat atatgaaaaa
     5161 tctaatttaa tgggggtaat gaatatgaga gaagtagtaa ttgccagtgc agctagaaca
     5221 gcagtaggaa gttttggagg agcatttaaa tcagtttcag cggtagagtt aggggtaaca
     5281 gcagctaaag aagctataaa aagagctaac ataactccag atatgataga tgaatctctt
     5341 ttagggggag tacttacagc aggtcttgga caaaatatag caagacaaat agcattagga
     5401 gcaggaatac cagtagaaaa accagctatg actataaata tagtttgtgg ttctggatta
     5461 agatctgttt caatggcatc tcaacttata gcattaggtg atgctgatat aatgttagtt
     5521 ggtggagctg aaaacatgag tatgtctcct tatttagtac caagtgcgag atatggtgca
     5581 agaatgggtg atgctgcttt tgttgattca atgataaaag atggattatc agacatattt
     5641 aataactatc acatgggtat tactgctgaa aacatagcag agcaatggaa tataactaga
     5701 gaagaacaag atgaattagc tcttgcaagt caaaataaag ctgaaaaagc tcaagctgaa
     5761 ggaaaatttg atgaagaaat agttcctgtt gttataaaag gaagaaaagg tgacactgta
     5821 gtagataaag atgaatatat taagcctggc actacaatgg agaaacttgc taagttaaga
     5881 cctgcattta aaaaagatgg aacagttact gctggtaatg catcaggaat aaatgatggt
     5941 gctgctatgc tagtagtaat ggctaaagaa aaagctgaag aactaggaat agagcctctt
     6001 gcaactatag tttcttatgg aacagctggt gttgacccta aaataatggg atatggacca
     6061 gttccagcaa ctaaaaaagc tttagaagct gctaatatga ctattgaaga tatagattta
     6121 gttgaagcta atgaggcatt tgctgcccaa tctgtagctg taataagaga cttaaatata
     6181 gatatgaata aagttaatgt taatggtgga gcaatagcta taggacatcc aataggatgc
     6241 tcaggagcaa gaatacttac tacactttta tatgaaatga agagaagaga tgctaaaact
     6301 ggtcttgcta cactttgtat aggtggagga atgggaacta ctttaatagt taagagatag
     6361 ttttagatta taatatttac aaattaaata tttaattatg aatttaacct gtaagaataa
     6421 ctatttgtta ttcttacagg tttttatagt taaaaataat tattatctat taaacaaaaa
     6481 tagtgtaaaa tatataatat catgattttg aagatataaa aagtaggaag atataaaaga
     6541 aagtaggtaa aagtttgttt aaaaaagaga atcaaagata taatcttata tcgtatacaa
     6601 atgaagaaga aatgacttta aaagaagttt tattggacaa gctaaatttc tcagttaggt
     6661 cattatcaaa aatgaagagg gaaaagagtg ttttagtaaa tggagtatat aaaaaaccga
     6721 gtttaaaggt gtatagtggt gatttaatag aagtaaaaat agacgaggaa aaagcaaatt
     6781 ttgaacctca agatttaaat ttacagataa tttatgatga ttttgatata attatggtga
     6841 ataaacctcc gtttatggta gttcacccaa caaaaagcca ctatgataaa acaatagcaa
     6901 atggtattag ttactatata gataatcaaa aagaaaatgt aaaaataaga tttgtaaata
     6961 ggttagatat gaatacttca gggttagtta tagtagcaaa aaatgcttat gcacatcata
     7021 ctttgtctac agcaatgagt gaaaataaag ttgaaaaaaa gtatataaca gtagttgatg
     7081 gaattataaa agaaaatgag ggaactatag atgagccaat ttatagacct acagaagatt
     7141 caataaagag gataatagat gaaagaggac agtcatctgt tactcactat aaagtaatag
     7201 aaagactaga aaatgcaact gtattagaag taagcttaga aactggtaga acacatcaaa
     7261 taagagtaca tatggctcat ataggtcatg gaataatagg tgatgagttg tatggttatg
     7321 tagatgaaga gctaataaat aggcaagcac ttcatgcata taagttagag tttgaacaac
     7381 caagaacaaa agaaaaattg aaatttaaag cagatatacc agaggatatg aaagagttaa
     7441 tatcgaaatt aagataatgg ggtgatatat atgataatac ataaatttat aatacatgtt
     7501 ttggacaaaa atagtgatgt tccaatatta aatgactttg aaggcaaagt taatcaagaa
     7561 gtagatggtt ttttccagaa agctataaaa agaattgcaa aagatgagga tttaagaaaa
     7621 ggtgttttta aagattataa tgacaattta attaaaaact gttgtgaaca aataatatat
     7681 gatgaaagta cttttttgaa aaattctaaa gaaattgcat cttacttgtt tgatgttatg
     7741 aaaattaatg ctgcactaga atcttgtgat ttggcaattt gcttatacac tattaaagat
     7801 gaaaagagtg tagcaatact gaaattagat tataaaaagt tgtacactca ttcaattgaa
     7861 tttgtggatg ataaatttaa tattcaaatg gtttcaaatg aaattggtat cccagagact
     7921 ttaagacaaa agcaaggagc tttgattagt ctaagtggta taaatgatga gtttcatcta
     7981 aggcttttag ataaggatgc tgaaaaagaa gggtcagaat ctaagtttgt aacagaattt
     8041 ttaaatgcta aaaaaataga tgatgataag tacaagacca aggtatttaa aaatacagct
     8101 gaaaattgga taaccaatgc tcttagtaat gatataaagc aagctgaaga tgtaagaagt
     8161 atattaaatt atacactgaa ggaaaaacat gaagttgata taaatgactt tgttgataat
     8221 tcaattaaag atgatgagtt aaaggatagc tttaaagagc atatggaaga aaaaggtctt
     8281 gatgaaagtt ttagtataga taaaaaatgg gttgagaaaa agcttaaaaa aagaagtata
     8341 aaaacggata atggttttga cataaaaggg aatctaactg attttgaaga cccaatgaag
     8401 tatactgtaa aacaaaatca agatggaact atagatataa taataaagaa tgttacgttt
     8461 tatgaagaaa agtaatttat atacttagta tataaattaa atataagagc gtgtatttta
     8521 tatattgtaa ataaatgtaa tctagtctgt gcgaactcta atataattta ttatgtagaa
     8581 gtttacacag actatttata tttgtagaca aaacgatact atttgttttc ttcgatatat
     8641 ttacatatat caccaataca tttaaagttc tcagcttcct cgtctggtat ttctattcca
     8701 aattcatctt caagggccat tataatttca acagcatcta atgaatcagc ttctaagtcg
     8761 tccattaaag atgcttccat agttatctca tctaaattgt ctacccctaa ttgttctgct
     8821 attatttctt ttattttctc aaacatttta agcctcctaa agcagtttta attaatttta
     8881 atgttagttt aatataatat caagaatttt tcaatatgga atctataaaa aataataaaa
     8941 ttttataaat gtagtaataa agtattattt gatgaataaa ctttattgat aacaaatata
     9001 acttaggagg aaatgctatg aaatatgtag gactattact aacttcagta ggaatgtttt
     9061 tacttatagc agtaaatttt tattataatt caataacttt agatatgcaa cgtatagagg
     9121 attatgtaat ggagaccaat ttaatccttg aagatgtagc tgaaaaggaa agttatgtat
     9181 ctaatgaaaa agaagactac atatcaaggt taatgcatgt aaagaaagga atagaaaatt
     9241 caaaaacatc cttcttgata gaaagatata aggagtacaa aataaaatca atagaaagtt
     9301 taatctatac tatatcagag gagaaaaaag attatctgga tgaagttgat agatacaata
     9361 aacttggtga aaaagaaata aataaactaa tcaataaaaa ctttttagaa gtaacttatc
     9421 tgtctattac tacatatatt taattaaaga gacaaaactt agtctcttaa tgattcaagg
     9481 aggattagtt atgacaaaat tagaagacaa tatggaacgt ttctttggag gatgcgaagg
     9541 atttttttgg aataaaatgt tgataataat agttatagta ttcttactat tatgcacaga
     9601 cattttagaa gacttattat gcgatgacaa tatatggata tgggttatat taatagtatt
     9661 gttattattt aacttcgatg atggttgttg ttgctaaaaa atattgatta ttaaataggt
     9721 aaaaccagta acttatacaa gttatggttt tacctttttt agttcaaatt aaaaaattta
     9781 aaaaaatagt aaaaatacat tagcaaaatg gaaaccaaat gcatagtata tgttaggagc
     9841 gaaaagctca acaaaatatc aacaggaggt aacgtataat gttagataga ttctttggag
     9901 aagaaggatg ttttggtgga ggaggatggt ggataatagt actattcttc ctattcttag
     9961 cttttggaga tagctgggca gaatttgaca taatggcatg gataccattc ctaatagttt
    10021 tattaatact ttgctgctgt ggtggatgct tagaataaga acacagttat taaaaaaagg
    10081 agggctttgt tatgttagat agattttttg gagaagaagg atgctttggt ggaggaggat
    10141 ggtggataat agtactattc ttcctattct tagcttttga agattgctgg agagacattg
    10201 acataatgtc atggatacct ttcttaatat tattgctaat aacttgttca tgtggtggat
    10261 tctttgatgg tgaagtaggt tgcggctgct aatacttaaa tcattagaat aaaaaaatgc
    10321 tggtatttta taccagcatt tttttatttt attttttaat atgatttaaa ttaagaatga
    10381 atattattaa atttgttgaa tatagggtat aatataaaat ataatgtatt taaatgtact
    10441 tgcttttatg gtaaaataaa aacttatata aatgtagtta aaataggaac cttcaaaaat
    10501 ttggttcagt tttagataaa tttagtaaaa taaggaggat gaagatgaaa ggcaatataa
    10561 cgataaaaga tgttgctaaa caagcaggag tgtcaatatc tactgtatct agagttataa
    10621 atgattcaaa acctgtaact gatgaagtca aacaaaaagt tttagaggtt ataaaagaga
    10681 ctggatatat accaaatcca cttgctagaa gcttagtaac aaagaagagt caattaatag
    10741 gggtaatagt tccagaagtt tcagattctt ttgttaatga ggtgttaaat gggatagaag
    10801 aggttgctaa aatgtatgac tatgatattc ttttagcgaa tacatactct gataaggaac
    10861 aagaacttaa gagtataaat ctattgagag caaaacaagt ggaaggtata gttatgattt
    10921 catggatagt tgaacaagaa catatcaact atatacaaaa ttgtggaata ccagcgacat
    10981 atataagtaa aactgctaga aattatgata tatatacagt aagtactagc aacgaagaag
    11041 ctacttttga tatgacagag catcttataa agaaaggtca tgaaaagata gcttttataa
    11101 tgacgagtaa agatgatact gttttagaaa tggaaagact tgctggttat gagaaagcac
    11161 tttcaaataa caatatagaa ttagacaaga gtttgattaa gtatggtgga actgattatg
    11221 agagtggata caatagtatg aaagaactat tagatgatgg aataatacct catgcggctt
    11281 ttgtaacagg tgatgaggct gccataggtg ctataaatgc tatatgtgat gctggatata
    11341 aggttccaga agacatatct gttgcaggat ttaatgatgt taagatagct agaatgtata
    11401 gacctaaact tactacagta tatcaacctc tatacgatat gggagcagta gcaataagaa
    11461 tggttataaa attaataaat aaggaattaa ttgagaataa gaaaatagaa ttaccttata
    11521 gaattgttga tagagaaagt gttacagaaa gaaaaaaata aaactattaa aatcaatcag
    11581 gtagtgccta aggcactacc tgatttttta tgtcaataat atcaaattta ataatagatt
    11641 aactaaaaat aattatctaa ttacttaaat agtttatttt tatctattca tgagggttag
    11701 actttttagt tttcttaaat aaacttttag attttttttg ttgactacct ggtgtatttt
    11761 ttggtgcagg tgaatttgac ttacctttag taggtttagc cttcttagtt ttagctggtt
    11821 tagtatctct ttggttttct gcatctgaga ttttttccat tatattatcc agttttttgc
    11881 gttgattttc atctaaaaat gctttgaatt ttgatactac ttcatctctg ccatctagat
    11941 tcttacctat ttttataagt tcctccataa gttcattttc tgattttcct ttgtaactat
    12001 cagccatctt ttctatttta tccttgtcaa tatttttttg tttagccatt ttttctaatg
    12061 actctgtatc taattttttc ataaaaaaac ccctttctat aaatatatat tgaattatat
    12121 tcatactatt tatatataga aataatattg aagtggtgaa ttgtgagtga tatataatat
    12181 aaataaaaat tcttatagat aatatatatg cactgagctt taaaaatgga acattaaaat
    12241 atgaaaaaca tactttaata tagggcaatt atataatata agggggaatt gtcttgaata
    12301 acttactatt tttgattgga gtgattgcat tgagtaatgg taatatctct atatttgatg
    12361 ataaaaataa atcaaaagga aataatagag ttagaaaaaa caaaaaaaat gcaaatgata
    12421 aactttcaca gtctacacat ggcaatttaa ggtttgatgc aaatgatata aaaagaggtt
    12481 taaggctcat ggatttaagt gaagaagatt tagaaatggg aatggaaata ataacaagaa
    12541 ctaaaaaata catgtcaaga gatgaaagaa aaatactaat aaaaatagaa agtgttttag
    12601 atttagttag aggaataaaa aaattaaata atatagatat ggtagataca gaagatgaaa
    12661 cggatttttt cagaaaaatg gatagtgatg ataaaaagaa tatgatgata aaggaaatta
    12721 tagatgtatt cccagaaaaa agaaaaaatt cagttgaaaa agctatagga atgaaaaaga
    12781 agatagattt atttgcagaa ttatttttac cagatgactt tggtgaaggt ggatttagtt
    12841 tgagctcttt ggcaaatata aacaatcttg gtagtatgaa taatctcaaa ttgttaggaa
    12901 gtcttttaag aggtgatgat agtaataatg atgatgaaga caataatgat gagttaaatt
    12961 atgaagacga agaattagat tgtgaagaca aagaattgga tgatgattat gatgagttaa
    13021 cagacgaagg tgaaatggag gataatgaag atgaattagt agaagataag aacacaggct
    13081 atgaaaatat agaaaatgat gaagatatag tatatgatga ggaactatac aacattaatg
    13141 agcaagaatc cgaatacaat tataatgaaa aaaatactaa aactagagct aagaaaaaac
    13201 gataattaac taaatacata aagaaaaaag gaagaaaaag taatgataga ataagattat
    13261 ctcaattact atataaaacc actttaaaaa attcaaaatt tattatataa taataatatg
    13321 aaatcctgaa acatacgata aggctttttt aaattcaacc tacaaatgaa atacgggaga
    13381 ccgtgtctgg taaattataa catgcctagt tagtttggac gttaaaggtt actggcaagt
    13441 cacccacctg ggagtgccta gggtgtgaat ttacttagcc accggtgttt cgggacaaaa
    13501 aaagtgctac tatttcaaat agtagtactt tttttattaa tatgcatttt tctactaaat
    13561 acgcctaatt attgataaat tcaaaaagca agtaacaatt aagattataa aagcatagta
    13621 ttaattaggc ttcaaaaagc caaagactta ataagaatcg cttacaggga gggcaatgaa
    13681 aaatgcaaga ttataaaaaa aataaaagaa gaatgatgaa tcagccaatg tctacaatga
    13741 atgaagaaga agtgtataca gatgaaataa attcagaaga catgagaggt tttaaaaaat
    13801 cacaccatca taatggatgt aatactgata ataagtgtga gtgccatgat gattgcaatc
    13861 catgcaaccc atgtaatcca tgtaaaccta acccatgcaa tccatgcaaa cctaatccat
    13921 gtgatgacaa ttgtggatgc catgacaatt gtaaatgtga ttgtgaacca tgtgaaatgg
    13981 attcagatga atgttttgaa aacaaatgtg gaccagaatg ctgtaatcct atatctccaa
    14041 gaaacttctc tgtatcaaat gcagtgccat ttgcaataga ggctaataga atatttgata
    14101 ctatgcaatt ccaaacattt acagatgcaa caggaccaaa tggagagcca ttaacttttg
    14161 aaacagaagt agtagaagta tttggttcag ttccaagtgc aggtcaagca agtgtaacta
    14221 tagaaaaaat atgcttaagt aatgatggaa tcgttataga cacaggaatg acaactttag
    14281 aagatttcga tttagaccca ttaggagata tagtaggaag aaactgtgaa acaacttttg
    14341 aatttgcagt ttgtggagaa agaaacgctg agtgctgtag acaaggaaaa ggcaaatcag
    14401 tagcttataa acaaagagga ttaactgtag cagttcgtaa tttagtacta gagctaagag
    14461 gtagatgtgg atgtacagag ttcgttgcat tagctttccc agcagttaga gcaggaggtg
    14521 gatgtaagag aagagttgat tatgtagaat ttacttttaa cacactttca gcaccaatat
    14581 gcttgccagc tgacggaaga gctgttactt taagacaaga atatcaaact aacttaactg
    14641 tagattgtat aggaaaatct atattaaaat tagaatgcaa cgaatgttgt gaacctttct
    14701 atgaattaat tataccaaat gatatagatt tagtactttg cttacaagaa acagttagca
    14761 cattaataag tgaacaaata gtagttttag catcaccaaa tccaatccaa ccaagacttg
    14821 ttgatacttt ctctaaagta tgtgattttt cgcaatgtgg acctaatcat ggaagtggaa
    14881 agccaagttg ccacagatag ataggtaaga tatatagaca gtttaaatta taaataaaaa
    14941 tcatctaagg aaggctacca aagtgtataa tttacattgg tagccttcac gtttttataa
    15001 ctagcgtata gattttagtg atttaaaaag cactatagta tgtaatagac aagtaaagat
    15061 tgatactatc gtcattgtgt atatgtatgc ataaatatta agtcctggaa ttggaagaaa
    15121 tacatataaa gctattagtt gaattaacat gccagtaata gtatttatac ttgaagctaa
    15181 ttcttttctt attgaatgta aaatgccaga tagagtttga tttagggaca taaatagtgg
    15241 aactaaaaac atagctttta gatattctcc tgcaagtgta tttttaaata ctaatataca
    15301 taaatctttt ccaaagaaat aaaaaaatat tgatgatagt atgcctacaa ataaagtaag
    15361 aagtatggag tatttgattt ttttaattac actcttacgc tttcttagag ccatttcctg
    15421 tgaaatagtt ggtattaaat ttacaataag cgctgaacca acagtaaaag gtaaaaatat
    15481 aaaaggcatt accatcccac tgacaatacc atacatactt aaagattgtt gataagtcat
    15541 tcctgataat gctaatcttg atggtatcat catagaactt atagattgca aaagtatgtt
    15601 tgacattcta ttacaagtta tgggaataga catttttaat gtttccatag aagaattata
    15661 aaaatctttt atgcttataa tatacctatt gtcaaatgat gaatctcgcc ataagcaaat
    15721 aagcataaat ataatattac tcaactcacc aattgatata cctaatagag ctatataaca
    15781 attcatagat ttattattta tgtacataac aagtaaaaaa acaaataata ttttaccaat
    15841 ttgttcaatg atttgtccaa tagcagggat ttttacattc tttatgccat agtaatatcc
    15901 tcttagtacg tttgaaagag ttattataac tatagcagga caaacagcta gtataaacag
    15961 atttaattta gcatctttta aaaatttaag tgataaaaaa gaactattga aagatgcaac
    16021 tatagatata attagtgcta cgaaaaatga tatatataaa gttgatataa ataaagcatt
    16081 agtattgtgt ctatcgttta aagcttttct ctttgcaact agacaactaa gtgcagtagg
    16141 tatacctgtt gttgtaacag ctaagcaaat catcaaaaag ttaaaaatca tatgatagat
    16201 acctaaacct gtttcaccta gtgctcttga taagaatatt ttatataaaa aaccaaatat
    16261 tcttacaata aaattggata taaataaaat aactgttgat agtactaaat aaggtacttt
    16321 caaaaaaatc acctccttat cttttaaata tattcacaag gaggtgtaaa aatttataat
    16381 tttataaatt ctggtttcaa tttattaaat ttagatacat atttaaatcc catatttttt
    16441 agtaatgaat atatgtagtc aaactcacat gcaacttgac ttatatgatg tgaatctgaa
    16501 cctgtggtta tgatttcccc tcccaagtct ttatacattt gaagtatata cttagatggg
    16561 gttaggtctg gaagattata tctataacaa gaggtattta tctcgatacc tttgccatca
    16621 taaatagctt gtttaagagt ttcttcaatt atatcagaaa atagtctatc atctaaaata
    16681 gtgtcataag gtgcatacct tttaatcaaa tctaaatgac ctaagacaga gtaatcttta
    16741 taattcttaa caatattata taaataaaga tagtaatttt cataaacttc atgttgagtt
    16801 ttatctttaa aataattacc aaggtataag tccattgtat ctatagcatg gatagaacat
    16861 attataaagt ccaatggata ttgatgtgct tcctttttgt atgtatccat taattgagtt
    16921 tgaactccaa attcaattcc tttttttatt gatattttat ctttatattt gctttgaaga
    16981 gtctctaaac tcttaaaata atcttcatag acaattgaaa aagaatcatc agcatataca
    17041 tcatagtcaa catggtctgt gaaacaaatt tcctccaaac cgagttcaat agattttttt
    17101 accatttctt ccattgtaga ttttccgtct gtagagaaac ttgagtgcat gtgatagtcg
    17161 taaaacataa atatttcccc tttttataaa aattttggta ttatatatac atactaaata
    17221 atatcaaata tacataaaag catcaataaa atataaatac aatgagcggt gataaatttg
    17281 gaaaaataca gaatagattt aaatgagaaa aaatcaacta aatatataca gatatttaat
    17341 catataaaag aattgataat aaataaagag ttagtagaac atgaaaaatt gcctccaata
    17401 agaaaactat caaatttttt acaagtaaat aacactacta tagttaaggt atacgagctt
    17461 ttagaaaatg aaggatatgt atataaaatt atagggagtg gtacttttgt atcaagttta
    17521 aattcttata aaagcggtat agataacagg aaaaataaga ttattgtgca aagagaagat
    17581 ttaattcatt ttgacaatgg gaatccatct aatgatatgt ttcctatcga ttcatttaaa
    17641 aatgctgtaa atatggcatt atctaaagat ggtagttcaa tatttgaata tgatgaaggt
    17701 cttggctctg aagaattaag agaaaaaatg gtagagtatc taagtgatgg aaatataaaa
    17761 actactaagg aaaatattca aataatatca ggtgctcaac aaggtataga tatagtttgt
    17821 aaaggactta taagttattc agatgttgtt tttatggaag aaccttctta caatggagca
    17881 attgaagtgt ttaaaagtag aggtgcaaaa ataatatcta tacctatgtt agatgatgga
    17941 attgatatag gtattttaaa attaaagttg gaaaaaataa aaccaagact tatatacatt
    18001 atgcctaatt ttcaaaatcc aactggaata tcttactcaa catataaaaa aaagaaattg
    18061 atagaattgg cagaagaaca tgatttttat ataatagaag atgattttat aagtgatttt
    18121 gtttttgaat caaaggacaa tagaacaatt agaagttatg atgataaaaa tagagtagta
    18181 tatataaaaa gcttttcaaa aatacttatg ccaggtctta ggataggaat tgtagaaatg
    18241 ccaaatgagt tgttaaaaaa agttttatgg gctaaatatt catcagatat atcaacacca
    18301 ggtttaattc aaaaatcaat gtattactat atgaataatt ttgattggaa gaattatttg
    18361 agtcatatag agaagatata tactgataag tataaattgg caaaaaactt gattaatgac
    18421 aaattgagtg gtaaattaaa agttagaaaa tcttgtggag gaataaactt ttttttagaa
    18481 ttaccaagag gatatacatc tcaagcattt acaaatttta tgttaaacaa aggagtgtca
    18541 atgcttccag ggacttattt ttttgacaat ctagtggatg atagattctt taggataaat
    18601 atagctcgcc caagcatgga agaactagaa aagggaatat ctataataag tgataatata
    18661 gatgagtttt tttgtgaata taaaaacaag ttagatataa aaagcaataa attattttat
    18721 taaataaata gaggtttttt aggaggaaaa aatgtttgac aagaagaaat tagataggat
    18781 taatgaatta gcaaagaaaa ataaagaggg aatcctttct gctgatgaaa tcaaagaaag
    18841 agaaatttta aggaaagaat atttagaaaa ctttagagcc cactttagat caagactaga
    18901 tagtgtcaaa gtagtttcac cagaagaata tgagcaatac atgaaaaaca ataaaaatta
    18961 gaacttagta ggaggaaaat atgaacttaa agtacgaatt taagaatgct tgggattttg
    19021 aaagaaaaga aaatactatt gaacaaatta tgcaatattc tagcaattat atggagtttt
    19081 taagtaagtc aaagactgaa agactttcag taaaagaaat tataaaattg gctaaagaaa
    19141 ataactatat ttcaatagat gaagctatgg aaaaaggaag cataagttgt ggtgataaga
    19201 tttatgttat aaataaagag aaggcagtag cactttttgt aataggtaaa aattatatag
    19261 aaaaaggtat gaagattata ggaagccata tagattcgcc aagattagat ttaaaaccaa
    19321 atccactgta tcaagagagt aatctaggtt tttttaagac tcattattat ggtggaataa
    19381 aaaaatacca atggacggct attccacttg ctttacatgg tatagttata ttaaatgatg
    19441 gaacaaaagt ggacatatct attggggaag aagatagtga cccagtattt tgtgtaactg
    19501 atttattgat tcatttggct ggagaccaaa tgcaaaagaa actatcagaa ggaattagtg
    19561 gagaagcttt aaatatatta attgggaata tgcctttaga agatgaagaa aaagaaccta
    19621 taacagcaaa tatattaaag attttaaatg agaaatacaa tatagttgaa gaagatttat
    19681 taagtgctga aattgaagta gttccagctg gtaaggctag agatttaggg cttgataggt
    19741 ctatggtgtt gggatatggg catgatgata gggtatgttc atatgctgct gtaaaagcta
    19801 tactagaaac tgaacaacca gaatttactt cagttgcatt atgtgtagat aaagaagaaa
    19861 ttggttctaa agggaataca ggtatgcact caaagttttt tgaaaatact gtggctgaac
    19921 tgattgcttt agaaggagat tattgtgata ttaaagtaag aagagcttta gcaaatagta
    19981 aggtattatc agctgatgtt tctgctggat atgacccaaa ttttggagaa gcatatgaaa
    20041 agagaaattc ggcatatatg ggaaatggag tagttttaac taagtataca ggttcaagag
    20101 gaaagagtgg atgtaatgat gctaatgctg aatttatgtc agaagtaaag agaattttta
    20161 ataaaggaaa tgtagtatgg caaactgctg aacttggaaa agtagaccaa ggaggaggcg
    20221 gaactatagc ccatatattg gcaaatcaag gtgcagaggt tatagactgt ggtgttggag
    20281 ttttaaatat gcatgcacca catgagattg tctctaaagt agacatatat gaaatgtata
    20341 agggatataa ggcatttttt aatataaatt tataagtaaa aatataaatg taataagtga
    20401 ataaaggctg ttgtataaac aatttaatct gttgtacaac agtcttattt ttatgtaaaa
    20461 atagcaattt gtcacactag agaaaataaa agaaaatact gtcaaattaa aaattattta
    20521 aaaaaaaatt gacaacgatt tcatcttgtt ataatatgta ataaagaact tgttatataa
    20581 tgttataaaa atgttacata gggaggtgag caaaatggat gaaaagagac aaaaaaaaga
    20641 aatggattta gaagtaaatt cctttgttcc tttatatcaa caactatatg acaacataaa
    20701 aaaacaaata gcatctggca tatataaacc aggagataaa cttccatctg aaggagactt
    20761 atgtaaagaa tttaatataa gtcgtataac tgtgagaaat acgacttttt tatataaaat
    20821 agcaaaataa cagtgtttga gcaaaaaaaa gaatgttttt tatcgttttg ccacctgttt
    20881 gccaccataa cttttttcgg tggcaaatta ttgtaaaatg ccctccaaaa tatctattgt
    20941 ttcactttcc attttacttg taacatgtga atatgtgtcc atagtggttg ataattgact
    21001 atgacctaat ctttgttgta tatattttac attagctcca ttttctaata ataatgtggc
    21061 atgtgtatgt cgcaagcaat gaaaattaaa gttaatattt agtttcttgt taatagttct
    21121 tatagcagcg tcaatattgt tgtgatttac aaatgagcca tctttttttc tacaaaccca
    21181 atcatactca gtttctttat accattttcc aattttaatt ttttgttttt tttgattcaa
    21241 tttttcctct tttaatatcc tagacaaagt atcacctatt ttaatatctc ttattgatgt
    21301 ttttgtctta ggtgatgcta attcaaattc tgaaactttt ctctttatta aattttttct
    21361 aactttgatt atattattat ctaaatcaac attatcccaa caaagaccta atatttctcc
    21421 ttttctcatg cctgtatgaa accctataag tagaggaata tagatatttg tatttttagg
    21481 atatatttct agtattttat tgaactcatc tagtgttata gtcttgttat ctgactcatt
    21541 ttttaccttt aaaacatttt ttggtatact ggcatattga acaggatttt ccttaatgag
    21601 tttgtaaggg taaacagcag attttaaagc agcatttaat acaacataaa tcgcttttaa
    21661 aactcctttt gtgtagtgtt tttcttctcc gttttgtgtg tatgtctctt ttgatttatt
    21721 atttaaaaat tcttgaataa tagctggatt tatagatttt agcttacatt taccaagtct
    21781 aggttctata tgattttcaa ttaaatttct ataactttcc tgagtattgt atttgcaatt
    21841 aagaagtaca tactctttgt accaaaagtt aagatagtct gataaactca tatttgtttc
    21901 atcaaacact ataccagcat tttcatattc atttattgca tcacggagcg atttttcagc
    21961 ttctttctta gtgtttccac caactctttc tacctttttt ctcttgccat ctactatacc
    22021 tgcatcaaag tagtaatacc acttctttcc acgttttctt acgccgcctt tcataaaatc
    22081 tctcctctca aaagcttttt ttatgtaatt atataataac atatatcaat ttaaataaaa
    22141 taaataaaaa agactataag aaacaaagtt ttatgtttat aattaatagt cttttttatt
    22201 tagatacata tttaatgttt tttatcacgt gattccagca ctttttctgc caaataaaca
    22261 actatttttt catactcaat atcagtttct aaagaaaagt caacaatagc aggtatcgtc
    22321 atatctcctg catatttatt acctaaccaa ttttcaatta attccaattc ttctttgctt
    22381 aattcaaatt ttttcttttt attaaaactt aagttcaatt tattcacctc aaataatatt
    22441 atatattaaa aatagtattt gatagaaaca ttcgaattat ttatgtaatt ttcttttttt
    22501 aatatattca tcagcaaaat aatttactaa atatttttcc tccaatccta aagctatttc
    22561 taattcaata agagcaggga tagttaaatc tttattctca ttattttcca acctagaaat
    22621 ttgacttctg tggcaaccaa ctctttttgc taactctatt tgtgttaatt tcttcttttt
    22681 tcgtaactct tttaacatat atttaaccta ccttttagat aattttatat atgttagttt
    22741 gtgtaatttc ttgtaataaa tgtgcaatat tcgcacactt tttgttggaa atttgtgcta
    22801 gaatgtagtt aagaaataac tttatctaga taaagcaaaa ataatagaag ctataaaata
    22861 taataatatt ttacagttat tttatagttt taagctttga aaaagaggtg gttgtaacta
    22921 tttttaagaa cgtatgtt
//
LOCUS       DAELJO010000045        22935 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELJO010000045 DAELJO010000000
VERSION     DAELJO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.045"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            491..3544
                     /locus_tag="KPB88_001502"
     CDS             491..3544
                     /locus_tag="KPB88_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0053788.1"
                     /translation="MKEILKRLCTGILALATVVTALPSTPVHAESKQYWTESDERVGI
                     VERVNNDGSITETFNEGHMKVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTASHNEISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDNSGNTEVAEVKSTTVYIKELTAPMGFKVDKT
                     IYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGKKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELVIDGKRVENDYTFVADSETMKVEVAFTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKESEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENVELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFKENPEVPEEPEKPETPQTPE
                     KPSRPTDSPKTGDNTNLYGLLALLLTSGAGLAGIFFCKRRKTKKS"
     gene            3783..4109
                     /locus_tag="KPB88_001503"
     CDS             3783..4109
                     /locus_tag="KPB88_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0053789.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            4125..4508
                     /locus_tag="KPB88_001504"
     CDS             4125..4508
                     /locus_tag="KPB88_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG0053790.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVYLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGNSQNNNPQGHPKK"
     gene            4602..5024
                     /locus_tag="KPB88_001505"
     CDS             4602..5024
                     /locus_tag="KPB88_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0053791.1"
                     /translation="MMDLKDINHQPTLEDIKSYISNETFNQFFAYMNEEYKVLCKIEY
                     SKDVYFKGWNVKLRKAGKGLCVIYPKQGYFTILVVIGAKEQIRFNELYTTLSKEMKEI
                     YNNTKEGNNQRWLMVNIKHKDELYDDILKLIKIRRESK"
     gene            5106..6500
                     /locus_tag="KPB88_001506"
     CDS             5106..6500
                     /locus_tag="KPB88_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0053792.1"
                     /translation="MRMILNKGHRIRASDKNLVYHFSIGTLLFVFVAGLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEVLLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKQHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTISSLAQARQ
                     DGTATCEAKGDGTD"
     gene            6684..7880
                     /locus_tag="KPB88_001507"
     CDS             6684..7880
                     /locus_tag="KPB88_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0053793.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLHTLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAISDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLDEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            7893..8036
                     /locus_tag="KPB88_001508"
     CDS             7893..8036
                     /locus_tag="KPB88_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG0053794.1"
                     /translation="MWELVKDFLLVSVGTGIGVVLMCILTVGKETDRYMEKIKENKES
                     EDN"
     gene            8037..8258
                     /locus_tag="KPB88_001509"
     CDS             8037..8258
                     /locus_tag="KPB88_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053795.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            8342..8791
                     /locus_tag="KPB88_001510"
     CDS             8342..8791
                     /locus_tag="KPB88_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG0053796.1"
                     /translation="MSVNIAETLELLLDKNNKIAYKALQELQNESAETDCVYPYMNKL
                     SDMLDSDNSYVRTRGLTLLAHNAKWDKDYKIDEIIDKYLRHITDVKPITARQCIKLLP
                     IIAKYKPELRNDIISALHKANVCIYNDSMQPLVYKDIQKALKEIQKL"
     gene            8896..9192
                     /locus_tag="KPB88_001511"
     CDS             8896..9192
                     /locus_tag="KPB88_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0053797.1"
                     /translation="MNDIFKDMQAKVSCENISDLPSYRRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGCWWKRAEQLAKKQRHTGSTCR"
     gene            9111..9614
                     /locus_tag="KPB88_001512"
     CDS             9111..9614
                     /locus_tag="KPB88_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0053798.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALHTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNEPTFKHLSEDCQAYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPQ"
     gene            9632..10135
                     /locus_tag="KPB88_001513"
     CDS             9632..10135
                     /locus_tag="KPB88_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG0053799.1"
                     /translation="MLDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIAECLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPSSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            10296..10685
                     /locus_tag="KPB88_001514"
     CDS             10296..10685
                     /locus_tag="KPB88_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0053800.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPIALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQIFNETITAVRSVKYVPD"
     gene            10672..13122
                     /locus_tag="KPB88_001515"
     CDS             10672..13122
                     /locus_tag="KPB88_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0053801.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEPFNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKNYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLVHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRRENTPVSRNIADHIDSFTDYDFAHLLFSDGMV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVD"
     gene            13122..15308
                     /locus_tag="KPB88_001516"
     CDS             13122..15308
                     /locus_tag="KPB88_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0053802.1"
                     /translation="MKERIQSAFTKRKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYVPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSLDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIENNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFMRHRARRMERRLAGAVTAGGVAGAVAGSTVAGKRAERKNTASKGNRGNTTSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVHSAKEKTKTSVSDFKRGMVQ
                     EQQSRQSGRLEKQEQHRQNIADKRMELQKAQEARQVRKADKSATMGATHSHERPATAS
                     KPSAEKMQEVKRPATATTSKASEPVRTNAIKERLLSSGASDVKAPQSAQSVHRKNVEK
                     VVSQETRQNYVKDRRTKVQQTHTVQKNQQTIEKTRNLVTKKGQKKK"
     gene            15305..16204
                     /locus_tag="KPB88_001517"
                     /pseudo
     CDS             15305..16204
                     /locus_tag="KPB88_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
     gene            16857..18653
                     /locus_tag="KPB88_001518"
     CDS             16857..18653
                     /locus_tag="KPB88_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG0053803.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPVRSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIVNQKIKDARFLKLIRLFLKAGYMEDWKYHETYSGCPQGGIISP
                     ILANIYLNELDRHIMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKTATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTGIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTDSDRYEIHHVNKVKNLKG
                     KSEWEKVMIAKRRKTIVVCHKCHMAIHHGEKK"
     gene            18771..18881
                     /locus_tag="KPB88_001519"
                     /pseudo
     CDS             18771..18881
                     /locus_tag="KPB88_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            18898..19809
                     /locus_tag="KPB88_001520"
     CDS             18898..19809
                     /locus_tag="KPB88_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG0053804.1"
                     /translation="MFKKNKKPTENIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFAAIDMHTVHETETIQLRLNDTSGIENFVKNFGKSYYTWNNSKEAIEARTQA
                     ISNYLTKELQDLNVDTIRTDIPTSSTVTDVLVWNIEQLGTDTFSATYEVDQQIKEGEQ
                     TTAVKATYTVKVYVDADGDMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVTGNVLEPIGRDYLYSELVNPVFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIIM"
     gene            20368..>22935
                     /locus_tag="KPB88_001521"
     CDS             20368..>22935
                     /locus_tag="KPB88_001521"
                     /inference="COORDINATES: protein motif:HMM:NF012321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0053805.1"
                     /translation="MNLFMKNFTHKSSGYLCKKNHRIKKWNKIVIVALSIILLSENMG
                     TTLTVTATERVEATSVDTQKRVVDIDTKNLYVDLMANELYPNATTSRYSGRIWADKSV
                     FADTSTIIDGKSFSNDSDFLIAGSFLGSTRAVHRNQKTPIDLMVILDSSESMAVTGTN
                     PNLITNQRMPNAVKALNTLFNAVKEHSTDSRISLVRYSSSVDLILPLKSYTWEGDCLS
                     TQKKGNSYEVSINCNESTANFSTGGGTYTQGGLYEGMQTLANSTDTKVTDSTGNLIVR
                     QPVVIVITDGQPQRFTANNWWEPLASIDNSNGTGNGRAACALATIMTGAYWKQQITQH
                     YGITPFIYTIGIDIENTQASPTLTSYVEAVLNPDKGFRAVDGIDGTDIPDTSKPNFQQ
                     SVGYAYNAWEKYSKGEEFSLPDSKAVKQSFNHPDNDITTVSYVDKYYPTVSDELGGIF
                     EDIIENLESEAFHPTDESTGGQQSAMIYRDPIGKYMEVKDVKGVLWDGTLYNAVKNQN
                     GTYTLTLAEGETTPKHPITGKEIDINQMTIKVEHTITDGKTTETFVVEIPNECLPLRY
                     DEITVKDGVMIDYTSSRYTTTPLRVLYTVGIQDSIKTDGKIDIEKVDEAYKTANSNTD
                     GSVNFYSNLYVKANNPTGDDRYDNGAIGDTTVQFTPSIHNRYYFFQKNRQIYFDESCV
                     KPVPADYVTDNHLPDGSYYFKIDYYVSDDTSHGYDGSHSGYTETVVERSAEQLAGAVD
                     TIDGKVYTKIGASRMGRLDFFSRQKINNKTETAFYSFVPHIENDTYEMTIFLGNNGRL
                     TTAVPTANDTGALTVSKIVTGNAGDKNKDFNFAVTLSDKTISGVYGDLKFTDGVATFT
                     "
BASE COUNT         7659 a         4146 c         5178 g         5952 t
ORIGIN      
        1 gattaaacca gtagattgaa tgaattaata gattaaatta atatataaaa tatttaagtt
       61 ataattaatg tattagacaa aataaatact attaagaact attaaaatat agattaaaga
      121 taataacata taataaataa aaaagtaaga ttgtgtagat tatataaaga ttatttacaa
      181 ttaatacaat cttgcttttt ataagaaaaa cgtattaaaa atctgattat ccgctaaaac
      241 acagtatcta tcagcatttt caaggaaaca aacacatcaa tttactactt atttactacc
      301 attgaatgag ccttgatacg caagttttcc tagatatgca aagatatggt acagcacatc
      361 agtattgaaa gaagaaaaat tgaataactc atagactatg gaacgcctaa aatgacgttc
      421 cttttctatt tccagccgtt cttattggac ggctatttta ttacccaaaa tgaaaggaac
      481 atttagactt atgaaagaaa tattaaaacg attatgtact ggcatacttg ctcttgcgac
      541 tgtcgttact gctttaccga gtacacccgt ccatgcagaa agcaagcaat actggacgga
      601 atcggacgag cgtgttggta ttgtagagcg tgttaataac gacggttcta ttactgaaac
      661 ctttaacgag ggacacatga aagttgaagg cgaggacgct tactgtattg acattaacac
      721 agattttaag aacggctata agaccagagc tgacgcaagc tcccgtatga gtgccgacca
      781 gataagtgat gttgctttat ctatcgaata tgtgaagcaa tacaccgcca gccacaatga
      841 gataagcaac aatcacgcct accttttaag acagcttgtt gtctggcaaa gattgagtgt
      901 tcacttaggc tggcaatgtg ataacgtgcg agcttcttat gatgaaattc caaaggctac
      961 gcaagatgaa gtgtttgctg gtgcaaaagc ctttgtcaaa gaaaacaaag gacgctatga
     1021 atgtggcggt tatatctatt ctggcgaggg gcaggaatta gggcaattct gggcgaagtt
     1081 aaatgttgga aatgccaaac ttcaaaaaac ttccaataat accagcatta cagacggtaa
     1141 cgggaattac tctatcgctg gtgcgactta cggtgtcttt tctgataagg attgcacaaa
     1201 acagcttgct acccttacga ccgacaacag cggaaataca gaggttgcag aagtcaaatc
     1261 tactaccgta tatatcaaag aattgactgc accaatggga tttaaagtgg ataaaaccat
     1321 atattcctta aaagttgaag ctggaaagac agcgactttg aaagtatctg atacgccaaa
     1381 agttaccgat actttgattg agcttttcaa gattgatatg gaaacacaga aagccaaccc
     1441 acaaggtaac gcttctttag agggtgcaga gtttacatgg aaatattatg caggcttcta
     1501 caataaagac aatcttcctg ccgaagctac tcgtacatgg gttacaaaga caaccgctga
     1561 aacagacagt aacgggacaa cccattacat cacaaaatta gcagacgcat acaaggtatc
     1621 tggcgatagc ttctatatgc aggacggaaa agcggtttta cctcttggta cactaacggt
     1681 cgaagaaacg aaatcgccaa cgggctactt attagacggt gcatatatgc aggcgaatgg
     1741 tagtgaagaa cagataaaag ccttatacct tacgcagatt accgaggacg gcgaccttgc
     1801 cgtattaact ggaagtaacc agttttccgt atcagataaa gtcattcgtg gcggtgtcaa
     1861 aattcagaaa cgagatttag aaaccaaaga taccaaacca caaggaagtg ccactttgaa
     1921 agatactgcc tttgacatca tttccttaaa tgataatgcg gtattggttg agggcaagat
     1981 atacaagaaa aacgaagttg taaaaacaat ccatacggat attgagggta tcgcttctac
     2041 ttctgctgac cttttgcctt acgggaattt ccgtatcgtt gagaaacaag ctccagacgg
     2101 ttacttgaca gacggtgcga agccgattga ttttacaatc acagaaaacg gaaaaatcgt
     2161 ggacttaaca gacgaagccc actctatcta caatcagatt aagcgtgggg atattgaggg
     2221 cgtgaaaatc ggtgcaggca cacacaagcg tcttgctgat gttcccttta ggatcacaag
     2281 caagacaacg ggcgaaagtc atgttgtggt aactgacgat aatgggcaat tctccacttc
     2341 ttcaaaatgg gcttcgcaca agcacaatac caacgctggc aaaacaagcg aggacggtgt
     2401 atggtttgga acttctgaac cagacgacag caaaggggcg ttgccttatg atacttatat
     2461 cattgaggaa ttacgctctg acagcaacaa aggctttgag cttatcccac cgtttgaaat
     2521 cgtggtatca agaaataacc ttgtgattga cttagggact ttgactgatg aatacgaaaa
     2581 agaaatctca atccatacca cagcgaccag caaggacggc aaaaagacaa tccttgcagg
     2641 aaaagaaatc acgattgtag atactgtcaa attagacgga cttacaaaag gcacaaaata
     2701 tcagctcaaa ggctggcaga tgttaaagga agaaaacgct gaacttgtca ttgacggaaa
     2761 gcgtgtagaa aatgactata cttttgtcgc tgacagcgaa actatgaaag tggaagttgc
     2821 ctttacattc aatgcgtccg ctttaggtgg caagaacctt gttacctttg aagaattgta
     2881 tgatttaagc aatccagacg aacccgtaaa agttgcggaa cacaaagaca ttgaggacga
     2941 cgggcagacc gttttaatta cagagcgtat catcaagata catacgactg ctactgataa
     3001 ggacggcaac aaagagagtg aagctggaaa agacgttaca atcattgata ccgtaacctt
     3061 agagggctta gaagtcggta cacagtacaa acttgtgggc tggcagatgt taaaagaaga
     3121 aaatgtcgag cttctaatca atggaaaacg tgtggaaagt gattacactt ttactgctga
     3181 cagcgaaact atgaaagtgg aagttgcttt tacctttgac gctacttctc ttgacggcaa
     3241 acagcttgta acttttgaag aattgtacga tttaagcaat ccagacgagc cgaagaaagt
     3301 taccgagcat aaggatattg aggacgaggg acagacgatt acctttaagg aaaaccccga
     3361 agtcccagaa gaaccagaga aacccgaaac accacagaca ccagaaaagc ctagcagacc
     3421 tacggacagt cctaaaacgg gcgataacac caacctttac ggactgcttg cacttctttt
     3481 gacttctggt gctggactgg caggtatctt cttctgcaaa cgccgtaaaa cgaagaaatc
     3541 ataaggcggt aatggtatga tgaaagaaaa atcacgctct ccgccgaagc tgtcaaacgg
     3601 cagacttccg cttattctgg cttgtctgcc aacaacctgc aagagcacgg atagtcaagg
     3661 gtgcgtaagc atttatttta ccctttacta tctgggggct tgctggtact tctttaactg
     3721 ccagaataag aaatcacaat caattaactt atcaaatata gaaagaaacg aggtaaaaca
     3781 atatggaaat gaaatatgtc gtgccagata tggcacagtc ttttggaact cttgaatttg
     3841 caggcgaaag tgagccagtc tttgaaagag ataaaaataa ccgcagggtt ttagccagac
     3901 gaagctataa cctttattct gacgtacaga aaggcgaaaa tgttgtcgtg gaaattcccg
     3961 tgcaggctgg tgaaaaacat ttcaagtatg aacagaaagt aaaacttgtc aatccgaagt
     4021 tatacggcag aggttacgca atcggagata tgggacacac ggactatgta ctggttgctg
     4081 atgatattgt cgcagttgaa gaaaagtaaa ggagtgaaga atttatgaga ttatcaaacg
     4141 ggtttgtcat tgacaaagag aaaacatttg gagaattaaa atttacagcg gtgcgtgatg
     4201 tgtacttgca gaatgaggac gggacaccga gtacccagct taaaaagcgt atctatgatt
     4261 taaagtgcag tctgcatggt ggaattatcc ccgtgtctgt accgccagaa gtaccgttaa
     4321 gggaatttcc gtataatgca gttgtggagc ttgtcaatcc agtagccgat acggtatcac
     4381 gaaaaaccta tacgggtgca gatgtggact ggtatgtaaa ggcagaggat attgtgttga
     4441 agaataaagg taatcagaac acaggcaatt cacagaacaa caatccgcag ggacacccga
     4501 aaaaatagac tggctaacta gattttcgtt gtagaggtta tagagaactg ttataatatg
     4561 gatatgaaga aacggacaaa tcagaatttg gagaggtgac aattatggat ttgaaagata
     4621 taaatcatca gcctacgctc gaagatatta aatcatatat tagcaatgaa acattcaatc
     4681 aattctttgc gtatatgaat gaagaatata aggtactttg caaaattgaa tacagcaaag
     4741 atgtttattt taaaggttgg aatgtaaaac tgagaaaagc aggtaaagga ctttgcgtta
     4801 tatatccaaa acaaggatac tttaccatat tagttgtaat cggtgcaaaa gaacaaatcc
     4861 gatttaatga gttatataca acgctttcga aagaaatgaa agagatttat aacaatacaa
     4921 aagaaggcaa taatcaaaga tggttgatgg taaatattaa acataaagac gaactttatg
     4981 atgatatatt aaagttaatt aaaattcgaa gagaaagtaa ataacaactt catattcatg
     5041 cacataaagc agttagtcta tatgattgac tgcttttttc atacctagaa aggagtgatt
     5101 gatttatgag aatgatttta aacaaaggac accgtatcag agccagcgac aagaaccttg
     5161 tctaccactt ttccataggg acgcttctgt ttgtatttgt ggcgggtctt ctgctactga
     5221 atatcaaaca gctcatgcgt accgattggg agcatttcag cttgttagaa aacggcttga
     5281 cgctttcccc ttacaacttc ataaccatac tgatagcgac tggtgtttgt gcattggtcg
     5341 cttttctgta ttaccgcttt tgttacgaca gtttcaagaa gctcctgcac cgtcaaaagc
     5401 tggcaagaat ggtactggaa aataaatggt atgaagctga taccgtacaa gacagcggtt
     5461 tttttactga cctgcaaagc agatcaaggg aaaaaatcgt ctggtttcca aagatttatt
     5521 atcaaatgga aaagggactg cttcatattc gctgtgaaat cacgttggga aaatatcaag
     5581 accagctttt acggttagag gataaattgg aaagtggctt gtattgtgag ctgaccgaca
     5641 agaccttaca tgacggttat atcgaatata ccttgcttta tgatatgata gcgaaccgta
     5701 ttaccattga tgaagtacgg gcagaaaacg gctgtcttag actgatgaaa aatctagtct
     5761 gggaatatga cgcactcccc cacgctctga ttgctggtgg aactggtggc ggtaaaacat
     5821 actttttgct gacgctcatt gaagtcctac tgcatacaaa cgctgtcctt tacattttag
     5881 acccaaaaaa tgctgacctt gcggacttag ggacagtcat gggaaatgtg tatcacacca
     5941 aagaagaaat gatagattgc gtcaatgctt tttatgaggg tatggtacaa cgaagtgagg
     6001 aaatgaagca acacccgaac tacaagacgg gcgaaaacta cgcctatctg ggactgccac
     6061 cctgctttct tatctttgat gaatatgtgg cattttttga aatgctgggg acaaaagaaa
     6121 gcgtaagcct acttagccag ttaaagaaaa tcgttatgtt gggacgacaa gcagggtatt
     6181 tccttatcgt tgcctgccag cgtccagacg caaagtattt ctcggacggt atcagagata
     6241 acttcaattt ccgtgtagga cttggacgta tcagcgaatt aggctatggt atgctgtttg
     6301 gttcagatgt gaaaaagcag tttttccaga agcgtatcaa gggacgtggc tattgtgatg
     6361 tgggaacaag cgttatcagt gagttttaca cgcctttagt gccgaaagga catgattttt
     6421 tgcagactat cagctctctt gcacaagcaa ggcaggacgg gacggcgacg tgcgaagcga
     6481 aaggcgacgg cacggactag ccgttgctgg tgtggcgtaa gccacgccag caggtaaaac
     6541 ccctcggtat ctaacagagg ggtacgtttg caaatagaca aaagacgaaa aacataggaa
     6601 acataaggct tctggcatgg ttttttaaca aaaatcactt gtgaagtcgc cctaaaaaac
     6661 ttcacacttt acagattggg ggtatggttc tgaatgaaga acaatggata aaagaattac
     6721 gggagaaacg gattgcttac ggtatctcac aaggcaggct ggcggtggct tctggcatta
     6781 caagagaata tctcaacaag atagagagcg gaaaaatgaa gccgtcaaag gaacttcttc
     6841 atactctgca taaggaactg gcaaggttca atccagaagc accgcttacc atgctgtttg
     6901 attatgtgaa aatccgtttt cccacgctgg atatacagca catcatcaaa gatatattaa
     6961 aactgaatat caactatatg ctccatgaag attacggaca ttacagctat acggagcatt
     7021 attctttagg ggacatcttt atctatacgt cggctgacga agaaaaaggt gtccttttag
     7081 aattaaaggg gcgtggttgc agacagtttg aaagttacct gctggcacag caaagaagct
     7141 ggtatgactt cctcatggac gcactcatag acggtggcgt gatgaagcgt atcgaccttg
     7201 ctatcagcga ccatacgggc attttggata ttccagagct tgcggaaaaa tgcaggaaac
     7261 gggaatatat cggaaagtcc agaagttata agttttacca gtcgggcgag cttatcaagc
     7321 acagagagga cgacagagaa tatatgggac gtactcttta tcttggttcg ctgaaatcag
     7381 atgtgtattt ctgtatctat gaaaaggact atgagcagta cgtcaagtta gggacaccgc
     7441 ttgatgaagc cgacattatc aaccgttttg agatacggct tagaaatgaa cgtgcctatt
     7501 atgcagtacg ggatttgctg acctattatg acgcagagca gaccgccttt tctatcatca
     7561 atcagtatgt gcggtttgtt gatgaagaac cagacaagcg aaaaaatgac tggaaactca
     7621 atgaccgctg ggcgtggttt atcggcgata acagacagag cttgaagctg acgacaaagc
     7681 cagagcctta caccttagac cgcacattgc gttgggtgca aaggcaggta gcaccgacct
     7741 tgaaaatgct gaaaaagatt gataagggaa acggaacgga ctacatggaa acgattgaga
     7801 agcaggcaaa gctcacagaa aagcatgaaa tgataatcaa gcagcagacg acccctgcaa
     7861 aagatttggt ggaaagttag gagtgataaa aaatgtggga attagtaaaa gacttcctgc
     7921 tggtatctgt gggaacgggt atcggcgtag tcttgatgtg tattttgact gtcggcaaag
     7981 aaactgacag atatatggaa aaaatcaaag aaaacaaaga aagtgaggac aattaaatga
     8041 atttcggtca aaatttatac acatggtttt taagcaacgc acagagcttg gtgcttatgg
     8101 cgattgtggt tatcggtatc tacttagggt ttaagcgtga gttttcaaaa cttatcggct
     8161 tcttggtagt tgccttgatt gctgtcggct tggtgttcaa tgccggcggt gtgaaagatg
     8221 tactgttaga gctgttcaat aagattatcg gtgcataaaa ttttattgtg gtgctgatat
     8281 gagaatgtta taatgtggat atgaagaaag cgacaaatag gaagttgtaa aggagtatac
     8341 tatgtcggtc aatatagcag aaacacttga gttattgctc gataaaaaca acaagattgc
     8401 atataaggca ttacaggaat tgcaaaatga aagcgctgaa acggattgtg tctatcctta
     8461 tatgaataaa ttaagtgata tgcttgatag tgataattct tatgttcgta caagagggct
     8521 tacattactt gcccataatg caaaatggga taaagattat aaaattgatg aaatcattga
     8581 caagtattta agacacataa cagatgttaa gcccattaca gcaagacaat gtattaagct
     8641 gttgccaatt attgcaaagt ataagccaga attgagaaat gatattattt ctgcacttca
     8701 taaggcaaat gtatgtattt acaatgatag tatgcagcca ttggtttata aggacattca
     8761 aaaagcattg aaagaaatac aaaaattata gaagcacaaa tcccagtttg acaatttcat
     8821 atccatacac acaaagcagt tagtcttagg attgactgct tttttcttac ctaaatttca
     8881 gattggagtg atgtaatgaa cgatattttt aaggatatgc aggcaaaggt tagctgtgaa
     8941 aacatttccg acctgccctc ttacagacgt aaagtgtggc atgaaatgaa acggctgaac
     9001 cctgccgatt atgaagaaaa acagttagaa gatttttcaa aatatgtgtt tggtatgtca
     9061 tgccagactt taaaagatgt gatgaaacaa cagaaaggac gtgaggaaca atgcaggaaa
     9121 caagggtgct ggtggaaacg agcggaacaa ctggcgaaga aacaacgtca tactggttcg
     9181 acctgccgat agatgttgcc gagtttgaag aaaagttagg tgtcggtgca gaaagtgggg
     9241 attaccgcat tattgaaaag gtactgccct ttgctgatga agtccacgaa catacaagcg
     9301 tgtaccagct caacgaatta gattttatgt accgccagct ttcaagcgat atgcaggaag
     9361 aatatacggc acttcatacc gtgtttgaaa acttagaagc actttatatt tgcaggaatg
     9421 taattacggt ttatcccgac tgcaaaagca tgatagatgt tgcaagacaa aagctgatga
     9481 atgagccgac gttcaaacat ttatccgagg actgtcaagc atattacttt gactttgaag
     9541 cctacgcttc gcacttgcag gaacacggga aatttttagt aacggaacat ggtatctttg
     9601 aactgccaca gtaggaaatg aggtggtgct tatgcttgat gatatggcgg tttatattgc
     9661 gaacctcggt aagtacaatg aggggtattt agtcggtgct tggttcacat ttcccattga
     9721 cgaggaagat gtgaaagaaa aaatcggctt gaatgaacag tatgaggaat acgcaatcca
     9781 tgataccgat aacttcccta ttgagattgg ggagtatgtt tccattgatg aactcaatga
     9841 gatgtatgaa atgatagagg aacttcccga ttatatcgca gagtgtctgg acgaatttat
     9901 cggtcattac gggacgctgg aagaagtcgt ggaacacaag gacgatattt attattatcc
     9961 agactgtgaa accatgacag acgttgccta ctactacata gacgaattgc aggcacttgg
    10021 ggatattccc tctagtttgc agaactacat tgattatgaa gcatacggga gagatttgga
    10081 tatgggcggt tgcttcattg caacaagccg aggaatgtgc gagataccat attaacgctg
    10141 gaagatcagc gacaaggatt gttgctactg acagcgtatt tctctttaag ggacagtctg
    10201 aaacatggct gtccttttct attttgaaat cccacaaggg aataccttta tgccgtgagg
    10261 gacacggcag gaacaaagaa aggagctgaa agaacttgaa aaagattaaa agctacacgg
    10321 gtatctggaa cgtggaaaaa gtcttgtatg caatcaatga ctttaactta ccctttcccg
    10381 ttacctttac acagattaca tggtttgtga ttacagaatt tatcatcatt ctgtttgggg
    10441 atatgccccc actctccatg attgagggag catttctcaa atacttcggt attcccattg
    10501 ctctcacttg gtttatgtcg cagaaaacct ttgacggaaa gaagccgtac agttttttga
    10561 aatcacagat tacctatgcg ttgcgaccta aaatcactta tgcaggaaaa gccgtaaaac
    10621 tggataagca gatattcaat gaaacaatca cggcagtaag gagtgtgaag tatgttcccg
    10681 attaaatata tcgacaacaa ccttgtctgg aacaaggaca atgaggtgtt cgcttactat
    10741 gagttgatac cgtacaatta ttctttccta tccgcagagc agaaatttat cgtgcatgac
    10801 agctttcgcc agttaatcgc acagtcccgt gagggtaaaa tccatgcctt gcagattgcc
    10861 acggaaagtt ccatacgaag tatgcaggag cagtcaaaaa gattagtaac ggggaaatta
    10921 aaggaagttg cctatcagaa gatagacgag cagaccgaag cgttagtatc aatgattggg
    10981 gacaatcaag tggactaccg cttttttctt ggctttaagc tcatggtaac ggaagaacca
    11041 ttcaatctga agaacatcaa aaaatcggca tggctgacat ttaaggaatt tctccatgaa
    11101 gtgaaccaca cgctgatgaa tgactttgtt tccatgccga atgatgaaat caaccgttac
    11161 atgaaaatgg aaaagttact ggaaaataaa atctcaaggc gtttcaaggt gcgtcgcttg
    11221 gaaatcaacg attttggcta tctcatggaa catctttacg gcagggacgg tatcgcctat
    11281 gaagattatg agtaccagct accaaagaaa aactacaaga aagaaacgct gataaaatac
    11341 tatgacctta tccgtccgac aaggtgtgtg attgaggaaa gccagcggta tttacgattg
    11401 gtgcatgagg acaaggaaag ctatgtgtcc tattttaccg tcaatgcgat tgtgggcgag
    11461 cttgattttc cgtcaagtga aatcttctat ttccagcaac agcaattcac attccccgtt
    11521 gatacttcta tgaatgtaga aattgtggag aaccgaaaag cattaacgac tgttcgcaac
    11581 aagaaaaagg aattaaaaga tttagataat catgcctatc aagcaggaag tgaaaccagc
    11641 tcaaatgtgg tggacgcatt agacagtgtg gacgagctgg aaacggactt agaccaaagc
    11701 aaagaaagta tgtataagct ctcttatgta gtgcgtgtat cggctgatga tttggacgaa
    11761 ttaaaacgcc gttgtgatga agtcaaggat ttttacgacg acctcaatgt aaagctggtg
    11821 cgtcctgctg gggatatgct ggggcttcat tctgaatttt taccagccag caaacgatat
    11881 atcaatgatt atgtgcagta tgtaaaatca gattttctag cagggcttgg ctttggtgct
    11941 acccagcagt taggggaaaa cacgggtatc tatatgggct attccgttga tacgggaaga
    12001 aatgtgtacc tgcaaccgtc ccttgccagt caaggcgtaa aaggtacagt aacaaatgcc
    12061 cttgcttctg cttttgtcgg ttcgctgggc ggtggcaaat cgttctgcaa caatcttctg
    12121 gtgtattatt cggttctctt tgggggacaa gcggttatct tagacccaaa atcagagcgt
    12181 ggcaactgga aagaaacact tccagaaatc gcccatgaaa tcaatatcgt aaaccttacc
    12241 agcgacaagg acaatgcagg acttcttgac ccgtttgtga ttatgaagaa tgtcaaagac
    12301 gctgaaagtc tggcaatcga catactaacc tttttaactg gtatttcctc tagggacggc
    12361 gaaaaattcc ccgtacttcg taaggcggta cgttccgtta cccagagtga aacaaggggc
    12421 ttgcttcatg tgatagacga gctacgccgt gaaaatacgc cagtatcaag aaatatcgca
    12481 gaccatatcg acagctttac cgattatgat tttgcacatc ttctgttttc agacggtatg
    12541 gtagaaaatg ctatcagtct ggataaccag ctcaatatca ttcaagtagc agaccttgta
    12601 ctgccagata aggacacgac ctttgaggaa tacacgacca ttgaattatt gtcggtgtct
    12661 atgctgattg tcattagtac ctttgcttta gattttattc acagcgatag aagcattttt
    12721 aagattgtgg atttagatga agcgtgggca ttcttgaatg tggcacaagg cgaaacactt
    12781 tcaaacaagc tggttcgtgc tggacgagcc atgcaggcag gcgtttattt cgttacacaa
    12841 tcttctgggg acgtgtcaaa ggaaagtctg aaaaacaata tcggcttaaa attcgctttc
    12901 cgttctaccg acatcaacga gataaagcag accttagaat ttttcggtat cgacaaggac
    12961 gacgaaaaca accagaaacg cttgcgtgat ttggaaaacg gacaatgctt attgcaggac
    13021 ttatacgggc gtgtcggtgt ggtgcagata caccccgtct ttgaagaact gctacacgcc
    13081 tttgatacca gaccgcccgt acaaagaaat gaggtggatt gatgaaagaa aggatacaga
    13141 gtgcgttcac aaaaaggaag attttccact ttctcaaaat ggctctgttc gtggtggcac
    13201 tctcccttat cctgctttca cttctgggga cggtggctca tgcgacgggg cttgtggacg
    13261 acactatcaa tgcagaaaat ctttactcaa aatatcctct ttccaactac cagcttgatt
    13321 tttatgtgga taatagctgg tcgtggttgc cgtggaactg gctggacggt atcgggaaat
    13381 cggtacaata tgggctttac tgtatcacta attttgtctg gactatcagt ttatatttaa
    13441 gcaatgccac gggttatgtg gtgcaggaag cctataaact ggacttcatc aacgatatgg
    13501 cagacagtat cggaaaaagc atacagaccc ttgcaggcgt aacacagaac ggtttttcca
    13561 gctctggctt ctatgtcggt tttcttcttc tcattatctt agtggtggga atttatgtag
    13621 catacacggg actgataaaa cgagaaacaa gcaaggcact tcacgccgtt atcaactttg
    13681 ttgtggtgtt cgtactgtcc gcttcattta ttgcctatgt tcccgactac atcaagaaga
    13741 taaatgagtt ttcaagtgat attagtaccg cttctttaga tttgggaaca aaaatcatgc
    13801 tcccaaactc tgacagcgag ggcaaggaca gcttggactt gataagggac agcctgtttt
    13861 ctattcaagt agaacagccg tggctactct tacagtttgg gaacagcaac gcagaggaaa
    13921 tcgggacaga ccgtgtagaa gctcttgtat cggcaagccc agaggacgag gatggaaaga
    13981 ccagagagga agtcgtgaaa acagaaattg aaaataacga caacaacaat ctgacgatac
    14041 cgcaggtcgt gaaccgttta ggtatggtgt tcttcctctt gttcttcaac ttaggtatca
    14101 cgatatttgt attcttgctt acgggcatga tgttgttcag ccagatactc tttattatct
    14161 ttgcgatgtt cctgcctatt agctttttac tttctatgat accgagctat gaaagcatgg
    14221 cgaagcaggc aatcgtaagg gtatttaata ccattatgac acgggcagga ataacgctca
    14281 ttgtaacggt ggcgttcagc atttccagta tgttttataa catctccacg gactatccgt
    14341 ttttcatggt ggcgttcttg cagatagtgt gtttcgctgg tatttatatg aagttaggcg
    14401 acttaatgag tatgttctca cttaatgcca gcgacagtca aagcatggga cgcaggattt
    14461 tccgcagacc gtatctgttt atgcgacaca gggctaggcg tatggaaaga cggcttgctg
    14521 gtgcggttac ggctggcggt gtcgctggtg cggtggctgg aagtaccgtt gctggcaaga
    14581 gagccgaaag aaaaaataca gcttctaaag gaaatcgggg caataccact tccagcatgg
    14641 gacaacgtgc aggttcaaag gtgggtgctg tcttagatac gaaaaataaa gtgaaagaca
    14701 aggcaaacgc tgtcaaagag aatatcaagg atatgccgac acagacagct tatgctgtcc
    14761 attcggcaaa ggaaaaaaca aaaacaagtg tatcggactt caagcgtggc atggtgcagg
    14821 aacagcagtc cagacagtcg ggacgattgg aaaagcagga acagcatagg cagaatatcg
    14881 ccgataagcg tatggagctt caaaaggcac aagaagcaag gcaggtacga aaggctgata
    14941 aatcagcgac aatgggagcc acccattccc atgagcgacc agccacagct tcaaagccga
    15001 gtgcggaaaa aatgcaggaa gtcaaacgcc ctgccacagc gaccacttca aaagcaagcg
    15061 agccagtcag gacaaatgct atcaaagagc gtctgctatc ttcgggggct tctgatgtga
    15121 aagctccaca atcagcacag tcagtacata ggaaaaatgt agaaaaagtg gtatcgcagg
    15181 aaacacgcca gaattacgtt aaagaccgca ggacaaaggt tcagcagacg cataccgtcc
    15241 agaagaatca gcagaccata gagaaaaccc gtaaccttgt aacgaagaaa ggacagaaga
    15301 aaaaatgaaa ctgaaacata tcgctatcat tggcagtctg tttcctatcc tcttttctct
    15361 ggtacttttc tttggtgtct tgattagtgc ggacagcgac gacgagaaca gcaacttttc
    15421 ttctggcatt acgggtatga atttatccgc agaagtcttg aaacatcagc ctatggtaga
    15481 aaaatacgcc agagaaaacg gtatctccga gtatgtcaat gtgctacttg ctatcattca
    15541 agtagaaagt ggcggtacgg cagaagatgt tatgcagagt tcggaaagtc tgggactacc
    15601 gcccaactct ttagatacgg aaagctcaat caagcagggg tgtaaatatt ttgcgtccct
    15661 gctttcttcc tgcaaaaatc aaggaataaa tgacttgaat gtggcgatac agtcctacaa
    15721 ctatggcggt ggctatgtgg gatatgtagc aggaaaagga aagaaacata catacaatct
    15781 tgcagagagc ttcgcccgtg agaaatcggg aggaaagaaa gtaacctaca ccaacccgat
    15841 agccgttgcg aagaatgggg gctggcggta cggctatggg aatatgttct atgtggaatt
    15901 agtcaatcaa tatctgactg taccgcaggt atcgggagag ctggcacaaa aggtaatgaa
    15961 tgaagcgttg aaatatcaag gctggaagta tgtatatggc ggtagcaatc ccaacacttc
    16021 ctttgattgt agcggacttg tgcaatggtg ctatggaaag gctggtattt cattaccgag
    16081 aactgccgac ttgcagtatg acgcaaccca acatcttccg ctttcgcagg caaaggctgg
    16141 cgatttggta ttttttcatt ctacctataa cgctggttcg tatgtaaccc atgtaggaat
    16201 agtgcgtcca gagggacgaa agatagaagt agattaaaga aagggggaca gaaaaaaagg
    16261 cactaccccg taagataaca agggaaacca cctgcctaac cgaaaggcga aagctgtacg
    16321 ggaaaatagc acggcaggaa agcagaaagt tgttcaaaga caatagagca cgactgaact
    16381 gcaaggttaa gtgaatatga ggataaaact agatttgttg aatgtgagtt ccaaacctct
    16441 gttacgtgtg gacacaggaa acttgtctga aacctgtggt atgagtaacg ctgaaaataa
    16501 atgcctttca gtgggttatc aattaactca tacggtacgc tggagaacca gtagtaacgg
    16561 aacgaaagca tatccgacaa ttcacactag cccactactt tcgtcaactg gagattacct
    16621 aaaccagaat gcctacaatg gctatgcgta atgctcttgt agtgataaat cacaaggcta
    16681 tgctaagtga gatgacgctg aaaatctggc aaggtaacgg agctttcata gtagtctgag
    16741 aaccctgaaa agacagggaa agccgtaaaa tcggatacgg gaaaaccgtt cacatggcga
    16801 aggaaagcag tcttagcgaa ttaaaattag aaattgatta gggaggaaaa cctcaaatga
    16861 aaccaacaat ggagatttta accaaattac aggaaaattc aaaaaagaac catgatgaag
    16921 tatttacaag gctctatcgg tatttactcc gtccagatat atactatgtt gcatatcaac
    16981 acctctactc caacaaagga gcaggaacaa agggtgtaac agacgacaca gcggacggtt
    17041 ttagtgaaat atatattgaa aatatcattg aagccttaaa aaatgaaatg tatcaaccaa
    17101 aaccagtaag acgtacatac atcaaaaaat caaatggcaa aatgcgtccc ttaggactgc
    17161 ctgtctttac tgataagctg atacaggaag caatacgcat gatattagaa gcaatctatg
    17221 agcctatttt ctcagactac tctcacggtt tccgaccagt aagaagttgt catactgctc
    17281 ttgctcaaat aaaaaaagaa tttacaggag cacgctggtt tatagagggc gacataaaag
    17341 ggtgttttga taacatcaat catgcggtac ttgtggaaat tgtcaatcag aaaattaaag
    17401 acgcacgttt tctgaagctc attcgtttat tcttaaaggc aggttatatg gaagattgga
    17461 aataccacga aacatatagc ggttgccctc aaggtggtat catttcgcct attcttgcga
    17521 atatctacct taacgaatta gaccgtcaca taatgaagat aaagaaagag tttgatgtgg
    17581 caacaaaggc acgttataca ccagaatata caaaactggt aggactacgc caaagattac
    17641 acaacaaaat taaaaatagc aatggtatag aaagagaaaa gctgattgaa gaatataaaa
    17701 ctgcaacagc tcaaatgctc aaactaccag cgaaacaatg tgacgacaag aaaataaaat
    17761 atgtgcgtta tgctgatgat ttcctaatag ctgtgaatgg caatcggcag gattgtgaga
    17821 agattaagca agaacttaca gaatttataa gtaccacatt aaaaatggaa cttagtcaag
    17881 aaaaaactct gattacacac agtaatactc ctgctcgttt tctcggatat gatgtcagag
    17941 tacgccgtga ccagcagatt aaaccaaaag gaaaattcaa aacccgttct atgaataaca
    18001 aggtggaatt gagtattcca ttcaaagaca agatagagaa atttttattt tcaaatggaa
    18061 ttgtaaagca gaggtcagac aatgggaaat tagaacctat acaccgacca caactgctta
    18121 acaggacaga tttggaaata gtgactattt ataatgcaga attaagaggt atctgtaatt
    18181 attatggatt ggcaagcaat tttaacaagc taatctattt caattatctt atggaatata
    18241 gctgtttgaa aacacttgca ggaaagcacc gttccaaagt ttctaaaatt agagctatgt
    18301 ataaagacgg tacaggcaaa tgggctattc cttatgaaac taaaacagga ataaagaaaa
    18361 tgtattttgc gaattatgct gactgcaaag gaaagaagtt tactgatatt gttccacaaa
    18421 cagcaaaaaa ttattcccat gacgtaacaa cattagaaag ccgattaaag gcgaagatat
    18481 gtgaagtatg tgggtgtacg gatagcgaca gatacgaaat tcatcatgta aacaaggtta
    18541 aaaaccttaa agggaaatca gaatgggaaa aggtaatgat agccaaaaga cgaaaaacaa
    18601 tcgtggtatg ccacaagtgt cacatggcta tacatcatgg cgaaaagaaa taacctgtaa
    18661 tggagcaaag atggagagcc gtgtgcattg agaaatgcaa gcacggttcg gggagaggac
    18721 tgcacaaacc taccttagaa ataaggaaag gcggtgcttt cctactctac tatgtgggaa
    18781 ataatcagat gtaccatgca ggcaacccga taggatatgc ggatttgaat aatagttact
    18841 ggcaacagca cttaatcggt gcaggacgag taaaacaata gaaaggactt gaaaaatatg
    18901 tttaagaaga ataagaaacc gacagaaaat atcaaagaac caaaagaaaa aaaggtgcgt
    18961 actgttaagg taggcacaca taagaaaacc gtgattgcgt tgtgggtggt gcttatcgca
    19021 agcgtgagtt ttggggtgta taagaacttt gccgctattg atatgcacac ggtacacgaa
    19081 acagaaacaa tccagttacg attaaatgat accagcggta ttgaaaattt cgtgaagaac
    19141 tttgggaagt cctattacac atggaataac agcaaagaag cgattgaagc aaggacgcag
    19201 gcaatcagta actatctgac aaaggaattg caggacttga atgtagatac cattagaacg
    19261 gacataccga ccagctccac agttacagat gtgcttgttt ggaatatcga acagttggga
    19321 acggacactt tttctgctac ctacgaagta gatcagcaga taaaagaggg agaacagaca
    19381 acagcggtca aggcaactta taccgtgaaa gtctatgtag acgctgacgg ggatatggta
    19441 atcatacaga accctaccct tgcaccagca atcgaaaaat cagactatga accaaagaca
    19501 ccagaagcag acgcaagtgt agacgctgat accgtcaatg acgctaccgc atttctggaa
    19561 acattcttta aactgtaccc gacagccaca gaaaaagagc ttgcctacta tgtaacggga
    19621 aatgtgcttg agcctatcgg cagggattac ctttattctg aattggtaaa cccagtattt
    19681 acaaaggacg gggaaaatgt gaaagtcaaa gtagctgtga aattccttga taatcagaca
    19741 aaggcgacgc aggtatcaca gtatgagctt gtgctacata aggatagtaa ctggaagatt
    19801 attatgtaag acatttttgt tggcaggctc tattttcgga tatagcctgt ctttttcgta
    19861 gcaaatgtgc tggcactaac aagggattta attatatttt aatcaatgaa atcgtatctc
    19921 ttgacaattt taaatcctaa atttataata aattacgctt atctttgcat aagattaggg
    19981 tctatccttt gaacaaggtt gagaagactt aaaaaatcaa tttttataaa aatgaaagaa
    20041 atttataaaa gggggttgac agccatgctc atgaaatgct acgctcagac agacagacag
    20101 acagacagac agacagacag acagacagac agacagacag acagacagac agacagataa
    20161 agtctgcctt tttgtcgtac aatcaaatcg tgtttttaaa tgtataggca ctactatacc
    20221 tgtttttagg tataggggtg tcttttttgc gtttcaacat ctctataaag atgttctgat
    20281 gattgtcatg attcaagcat ttgagtggat aattcttata aaaaatattt tggacaaaaa
    20341 ttacattata aaaatgaggt gatataaatg aatttgttta tgaagaattt tacccacaaa
    20401 tcatcagggt atttatgtaa gaagaatcat agaattaaaa aatggaacaa aattgttata
    20461 gtagctttaa gcattatatt acttagtgaa aatatgggaa caaccttaac ggttactgct
    20521 actgaaagag tagaagcaac ttctgttgat acgcaaaaaa gagttgtaga tattgacaca
    20581 aagaatttat atgttgattt aatggctaac gaattatatc ccaatgctac aacaagtaga
    20641 tattcaggaa gaatatgggc ggataaatct gtgtttgcag atacttctac cataattgat
    20701 ggtaaaagtt tttccaatga ttctgatttt ttaattgctg gttcgttttt aggtagtaca
    20761 cgtgctgttc acagaaatca aaagacacct atagatttga tggttatact ggatagttcc
    20821 gaatctatgg cagttacagg aacaaatcca aatcttatca ctaatcagcg tatgccaaat
    20881 gcagtaaagg cactgaatac actatttaat gctgtgaaag aacacagtac agattcacgt
    20941 atcagcttgg taagatactc cagtagtgtg gatttaatac ttccacttaa gtcatataca
    21001 tgggagggag attgcctatc gacgcaaaaa aaggggaata gttatgaggt ttctataaac
    21061 tgtaatgaaa gtacagcaaa tttttcaacg ggtggcggta catatacgca aggaggtctt
    21121 tatgaaggta tgcagaccct tgccaatagt acagatacaa aggttacaga cagcacagga
    21181 aatttaattg tacgtcaacc agtagttatt gtcataactg atggacaacc acaaagattt
    21241 actgccaaca attggtggga gccacttgcg agtattgata atagtaatgg tacagggaat
    21301 ggaagagctg cttgcgcttt ggctacaatt atgactggag catattggaa acagcagatt
    21361 acacagcatt atggaataac tccttttatt tatactattg gtatagatat agaaaataca
    21421 caggcttctc ccacattaac atcttatgtt gaagcagttt taaatccgga taaaggattt
    21481 cgtgcagttg atggtatcga cggtacagat ataccagata cttctaaacc aaattttcaa
    21541 cagtccgttg gctatgccta taatgcttgg gaaaaatata gcaaaggtga agaattttcc
    21601 cttccagatt caaaggctgt gaagcagagt tttaatcacc ctgacaatga cattacaaca
    21661 gtaagctatg ttgataaata ttatccgact gtttctgatg aattaggcgg tatctttgag
    21721 gatattattg aaaatcttga aagtgaagct tttcacccaa ctgatgaatc gacaggtggg
    21781 caacagagcg ccatgattta tcgtgatcca atcggcaaat atatggaagt taaagatgtc
    21841 aagggtgtgt tgtgggacgg aacgttgtat aatgctgtca aaaatcaaaa tggcacttat
    21901 actttgactt tagcagaggg cgaaacaaca cctaaacacc ctattaccgg gaaagagatt
    21961 gacataaacc aaatgacaat aaaggttgag catactatta cggacggaaa aacgactgag
    22021 acatttgtag ttgagattcc taacgaatgt ctacctcttc gttatgatga aattacagta
    22081 aaagatggtg taatgattga ttatacttct tcacgatata caacaacacc tttaagagtg
    22141 ctgtatacgg taggtataca agattctatc aaaactgatg ggaagattga cattgaaaag
    22201 gttgatgaag cctataaaac ggctaatagt aatacagacg gaagtgttaa tttttatagt
    22261 aacctttatg taaaggcgaa taatccaact ggagacgaca gatacgataa tggtgcaatc
    22321 ggcgatacta ctgtccagtt cactccgagt atacataata gatattactt ctttcaaaaa
    22381 aacagacaga tttattttga tgaatcatgt gtaaagccag taccagcaga ttatgtaacg
    22441 gacaatcatc ttccagatgg aagttactac ttcaaaatag actattatgt aagtgatgat
    22501 actagccacg gttatgatgg cagtcattct ggttatacag agacagtagt tgaaagaagt
    22561 gcagagcagt tagcgggtgc agtagataca attgatggga aggtttacac aaaaatagga
    22621 gcttctcgta tgggacgttt agattttttt agccgacaga aaataaataa caaaactgaa
    22681 acagcgttct attcctttgt gccacatata gaaaacgata catatgagat gacgatattt
    22741 ttaggcaaca atggtcgatt gacaaccgca gttccaacgg ctaatgatac aggagcttta
    22801 actgtatcta agatagtaac gggaaatgct ggggataaaa ataaggactt caattttgct
    22861 gtaacgttaa gtgataagac aatcagcgga gtatatggag atttgaaatt tactgatggt
    22921 gtcgcaacat tcacg
//
LOCUS       DAELJO010000046        22799 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELJO010000046 DAELJO010000000
VERSION     DAELJO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.046"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            54..467
                     /locus_tag="KPB88_001522"
     CDS             54..467
                     /locus_tag="KPB88_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG0053806.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            669..1310
                     /locus_tag="KPB88_001523"
     CDS             669..1310
                     /locus_tag="KPB88_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0053807.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1334..2587
                     /locus_tag="KPB88_001524"
     CDS             1334..2587
                     /locus_tag="KPB88_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0053808.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIDLKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            2633..3283
                     /locus_tag="KPB88_001525"
     CDS             2633..3283
                     /locus_tag="KPB88_001525"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG0053809.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3334..3930
                     /locus_tag="KPB88_001526"
     CDS             3334..3930
                     /locus_tag="KPB88_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0053810.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            3943..4782
                     /locus_tag="KPB88_001527"
     CDS             3943..4782
                     /locus_tag="KPB88_001527"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG0053811.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5027..6181
                     /locus_tag="KPB88_001528"
     CDS             5027..6181
                     /locus_tag="KPB88_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG0053812.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNLKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6487..8184
                     /gene="ade"
                     /locus_tag="KPB88_001529"
     CDS             6487..8184
                     /gene="ade"
                     /locus_tag="KPB88_001529"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG0053813.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFGEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            8536..9000
                     /gene="bcp"
                     /locus_tag="KPB88_001530"
     CDS             8536..9000
                     /gene="bcp"
                     /locus_tag="KPB88_001530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG0053814.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            9010..9753
                     /gene="yaaA"
                     /locus_tag="KPB88_001531"
     CDS             9010..9753
                     /gene="yaaA"
                     /locus_tag="KPB88_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG0053815.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFAIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            9881..12538
                     /locus_tag="KPB88_001532"
     CDS             9881..12538
                     /locus_tag="KPB88_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0053816.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVAGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      12592..12694
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12804..14069
                     /locus_tag="KPB88_001533"
     CDS             12804..14069
                     /locus_tag="KPB88_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG0053817.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14099..14989
                     /gene="metA"
                     /locus_tag="KPB88_001534"
     CDS             14099..14989
                     /gene="metA"
                     /locus_tag="KPB88_001534"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG0053818.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            15480..15923
                     /locus_tag="KPB88_001535"
     CDS             15480..15923
                     /locus_tag="KPB88_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0053819.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            15954..17771
                     /gene="ftsH"
                     /locus_tag="KPB88_001536"
     CDS             15954..17771
                     /gene="ftsH"
                     /locus_tag="KPB88_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0053820.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            17949..20651
                     /locus_tag="KPB88_001537"
     CDS             17949..20651
                     /locus_tag="KPB88_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG0053821.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            20685..21383
                     /locus_tag="KPB88_001538"
     CDS             20685..21383
                     /locus_tag="KPB88_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053822.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            21740..22696
                     /locus_tag="KPB88_001539"
     CDS             21740..22696
                     /locus_tag="KPB88_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG0053823.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         9114 a         2426 c         4070 g         7189 t
ORIGIN      
        1 agaataaaat actttttaaa agaaagggac gagatatggg agattataat aaaatgcttg
       61 aattaaagaa taacttaggt atcagtgaaa gaaagttaaa atatccttgt atctataaac
      121 atttcaaagg gaaatattat gctacaatgg gtcttagcaa ggctattgat gatatagaaa
      181 acatatgtga aatatatggt aaagaaaatt taatccaaaa cagaaataaa tataaattag
      241 ttattagaca tactgaaaga gaagaagata tctatgttta tagggattta gatggaaact
      301 tttatcataa aaaagaagaa gatactaatg atttagtttt atacaagacc ttatatgatg
      361 atacaggaat atttgcaaga cctttggata tgtttttaga aaaagtagat acagataagt
      421 atgtcaattc aattcaagaa tacagatttg aagaagtata taaataatat aagataaata
      481 tgtttataaa tttagaaaaa ttctttaaca gtacaataat ttgcagaaat acaaatgaga
      541 tttattgtaa ataatatgtt agattagatt ataaaataga aacaaagtaa agtattatat
      601 gatataatta agttttgaga agtgataatt tatatttaat aaaaaatagg catatattag
      661 gaggacatat gggaaatact ttttatatag taagacatgg acaaacggat tggaatatat
      721 tgggaaaaac acaaggtcat ggtaattctg accttacgcc tcaaggtata gaacaggcaa
      781 aagaactttc tgaagatatt ggcaagtatt caattgatta tatttttagt agcgaccttg
      841 ggagagcaat gcaaacagct caaattttag gagataagct caatattgaa gttcaaaaaa
      901 ctgaggcact tagagaaatg ggattcggag tttgggaagg ccttttaata aaagaaattc
      961 aaaaagatta ttcagatata tatgcaacat ggagaaatga accacactta gtaaatatac
     1021 cagaaggcga aactcttaag attataaagg aaagagtaga tgcttttata aaggaactaa
     1081 atgaaaaata tgataataaa aatataattc ttgttacaca ttcaattaca ttgagggtta
     1141 tgttgttgtc atttttagaa tcaggtatgg aaaatatata tagaattaag caagataata
     1201 ctgctttaaa tatagtagaa tttaaagatt atggacctgt tatagtaaaa atgaatgata
     1261 caagtcatat aaaaaatcat gtaaagataa ataattctgc actggagtag gaatcaatta
     1321 taaggagaaa tttatgagta aagttatagt agttggtgga ggagcttctg gaatgatggc
     1381 agctttgtca gcttcaaaaa acaataatga agtaatatta gttgagagaa atggagaatt
     1441 aggaagaaaa cttagggcaa ctggtggcgg tagatgcaat tttaccaata atagagagat
     1501 agaagatttt tttgataaag ttgtcagtaa taaaaaattt ctctatagta gtttctatac
     1561 atttacaaat aaagacctta tttcgtattt tgaaagtaga aatttggaat ataagattga
     1621 agaagaaaat gaccacaaag tatacactaa aaatgataaa tctatagaag taatagaagt
     1681 attaaataaa gatttactca atcacaatgt aaagataatg tataataaga aagttataga
     1741 tattataact gaagaaattg atctaaaaga tgattctaat aaggacaagt caaagtatct
     1801 aataaaaggg attattttag ataatggaga taagatacta ggagacaagg ttattatatc
     1861 aactggagga gtgagttatt caaagacggg ttcagatggt tcaatgtata agattcttaa
     1921 aaagcatgga catacattaa acaaactata tcctgcttta gttccactta caatagagga
     1981 aaaatggatt aaagatttac aaggaatatc catgaagaat gtggaaatat cttgtaagat
     2041 taagaaaagg aaaatatcaa aatctggaga catgctattt gcccattttg ggataacagg
     2101 accttgtgtt cttataatgt cttcttatat taacaagatt attgaaaagg aaaaggtaga
     2161 attaaatata gatttcttac caaatttaag tactgatgaa atctctagta taatcagagc
     2221 atttccaaat aaaaatgttc taaataatct taagcaaata ctacctcaaa atttcttaaa
     2281 agagattttt tctctattat ctttagttga taaaaaggca agtgatttgt ctaaggcaga
     2341 tgaaattaga attattgagt atataaaaaa tatgaagcta acttgtaatg ggactacagg
     2401 tataaacact ggtatggtta cttctggtgg tatttcagtt aaagaaatta attcttctac
     2461 tatggaatct aaattagtta agaacttatt ttttacaggt gaagtaattg atatagatgc
     2521 agaaactggt ggatataatt tacaaatagc attttcaact ggatatcttg cgggtataag
     2581 tgtataaata taatttttta ataattaaat atgaataaat agaggtgtat atatgggtaa
     2641 tttagttata gctgtagatg gtccagcagg agctggaaaa agtacaatag ccaagatagt
     2701 agcaaaaaag ctaaatataa attatataga tacaggtgct atgtacagag cagttactta
     2761 taagtgttta aaaagtggtg ttgatgtcaa taatgaaaaa gaagttattc aaatagctga
     2821 aaattcggat atagatttta aagataacaa tatatatttg gacaaagaag ttataaatga
     2881 agaaataaga acaatagaag taagcaataa tgtttctaat gtagcaaaaa taaaagaagt
     2941 aagacagttg atggttgaag ttcaacgaaa aataggaatg aaaaattcag taatactaga
     3001 tggtagagat ataggttcat atgttttccc agatgcagat tacaaattct ttttagtagc
     3061 tactccagaa gaaagaggaa atagacgcta taaagaactt tgtaataaag gatataatac
     3121 tactttagaa gaagtaattg aggatataat aagacgagat gaaattgatt caaatagaga
     3181 atttgcacca ttggtaaaag ctaatgatgc actagaaata gatactacag gaaaaactat
     3241 agaagaagtt gtagaagaag ttgtatctaa aataaatctt tagtttttta tgcatcataa
     3301 tgaaaataaa tgtagtgttg aagggagata aacgtgaatt tttatagatt tgtaataaat
     3361 atttttaagg gtttttctaa aatatttttt aaatatgaag ttataggtgc agagaatatt
     3421 ccagatagag gaaatatagt aatagcatca aatcataaat cgaatcttga ccctatattc
     3481 ttggctgctg ccatagaaaa tagagaaata gctgcaattg caaagaaaga attgtttaaa
     3541 gtaaaaccat tgggatttat attaaaaaaa ttgcatgtta tgcctataaa tagagaaaaa
     3601 ccagatgtat ctacaataaa aactatttta aggtcagtta gagacggtta tgtattagga
     3661 atatttccag agggtactag aataaaagga gattcttttg gtaaggctaa ggcaggtctt
     3721 tctgtattta caataaaaag taaatctaaa gttgttccag tatcaataat atctaaatat
     3781 aaacttttta gtaaagtaat tgtatatatt ggagaaccaa tatcatttga agaacatttt
     3841 aaggaaaaat tgagtaatga tgaccatgaa aggatatcac aagagatttt agaagtgata
     3901 aaacaaaatt actttaaata ttcaaaataa gggggtaaag ttatgaacat aaaaatagca
     3961 aaaaatgctg gcttttgttt tggagtaaaa agagcaatga aaatggcatg ggatgaagtt
     4021 gaaaaaaatg attctggcat atatgcttta ggtcctctta ttcataacaa gcaagcagta
     4081 gcaaaatatg aagaaaaagg tcttaagact gtgaatgaaa ttgatacaat accaaatcat
     4141 gaaaatatga taataaggtc tcatggagtt cctgaaaata tatataagga agccaaagat
     4201 aaaaaattaa aaattgtgga tactacatgt ccatttgtaa agaaaataca tacagtagta
     4261 agtgaatatc ataataaagg ctatgaaata atagttattg gagatatgaa gcatccagaa
     4321 gttataggta taaatggatg gtgtgaaaat tcagctataa taataaagac cttagaacaa
     4381 atggaaaata tggaatttga taactccaaa agatattgtc ttgttgcaca gactacaata
     4441 aatccagagt tgtatataag tatagttaat aaactatcag ataaattaga agaaatagta
     4501 tttaatgata caatttgttc agctacaaaa acaagacaag aatcagctaa agagttagct
     4561 aaagaagttg attgtatgat agttatagga ggtaaacaca gttcaaatac tcaaaagtta
     4621 gtaaaggttt gtgaagactt agtaccaact tttgcaatag agactaaaga tgaattggat
     4681 gtaaatacgc ttaaaaaata taaaaatttg ggtattacag caggggcatc tacacctaat
     4741 tggataatag aagaagttgt aacattcctt gaaaatttat aatttgatag gtaataaaaa
     4801 taggtggaca ttgttataat ttatcataat atccaccttt gatttcatat tacatgtggt
     4861 aaatagataa cctaatatca aaaagtgtgt atcttaagat gaaaaactaa gagtataaga
     4921 tgcgcacttt ttaatattag aaattcaaaa gaaatattga tattgattat caatagaaaa
     4981 tagaatataa tataattgtt gcaaaattag tctaggaggt aaattaatgg aaatgttgat
     5041 atccgcttct gaagaggcgt ttttacatgt aggttctatg attggatttt ttattttgtt
     5101 atttggatat attaattata aaactagtgg aaattttact aatatcatat caaaaaacag
     5161 aaaatttcaa cccttaatag gtgctttaat tggagctata ccaggttgtg gcggaagttt
     5221 ggcaataatg ccattgtata taaatggaaa attaagcttt ggagcaataa ttgcaagttt
     5281 aatagctagt atgggagatg cagcatttgt acttatttca tcaaatatta aaatgtattt
     5341 ttttgtcact atagttagta caattacagg aataataact ggacaattag tggattactt
     5401 taaactagaa gaaaaattag ggttaaaaaa ccgtagaaaa tcagataaat atgatataag
     5461 taaaaataac cttaaagaaa ataaagaaca acataatcat gatgaaataa ttttagacac
     5521 tctagctaaa tctcatggaa atacaaatag acttgcattt ataataacac atggaaaggg
     5581 atataaaatt tatataggaa ttatagtaat cggttttata tttatgtcac ttgctcactc
     5641 tggacttaat cttcctataa ttgagaagtt acattcattg gaagaagtaa tagcagtgat
     5701 tggaatatta ttttcaataa tttatatgtg gtgttttaaa aaggtattta aaaattctaa
     5761 tcaaaaagag gaagaaaata aaaagatttc tttgcgagaa atgcttatcc attctgttgg
     5821 agaaatatca tttgtaatta catggatttt tattgcatat cttatttacg atttgataat
     5881 attaatttta ggtggagatg aatacttagt taaattagta ttatcaacag gtgtaataag
     5941 tgtatttata ggtgcaggtc ttggacttat tccaggttgt gggatacaaa ttgtgcttat
     6001 gtcattttat ttgaagggaa ctataccact tggtgcagtg atagccaact ctatttctca
     6061 agatggagat gcacttttcc cacttttagc aatggataaa aaatcttctt tatgggctat
     6121 gattattact actataccag caatacttgt aggtatgata gtttatgcat ttttaggtta
     6181 aatataatta tgaataaaga agtattaaaa aaataataaa gcacataaat ttatttataa
     6241 gaatagtgaa gattaaatat aaaaataaaa aattcaatat gaattaaatt attgcttaaa
     6301 tattaatgag aacatgggag ataatattat tcctcatgtt ctctctattt aattaaagga
     6361 attatggaac aaatttcaag tgatactttc tatcaaaact catatataat atatattaat
     6421 aaattaaact ataatacgta atacagtcag tatattgata taaaaaactt aggaggagtt
     6481 gtaaaaatgg gtaatttaaa aagacagtgt gatgtttcaa gtggaagaga aaaagctgac
     6541 attgtactaa aaaatgggac aatcataaat gtatttacag aagagttaat aacaggagat
     6601 gtagcaatag taggagacac tatcgttggc attggtgatt acaagggcaa tgtagaaatt
     6661 gattgtagca ataaatatat atcacctggc tttattgatg cacatatgca tattgaatct
     6721 acaatggtaa tgccaattga attatcgaaa aaattattaa aatcaggtac gacaactatc
     6781 atagccgacc cacatgagtt ggtcaatgta aaaggagcta gtgcaataga ttttttatta
     6841 gaatcgacaa aagatatacc acttaatgta tatattatgg tgccttcatc cgtacctgca
     6901 acaagttttg aaactaatgg agttggtaaa tttagtgcaa aagacatgga gagttatgta
     6961 aataatccta gaattttagg tttaggagag gtaatgtgtt ttaatgatgt tataaattct
     7021 gaaaatgaaa tcttggataa actagagctt tttaaaaata aagttgtaga tggacatgca
     7081 cctaatataa atggtaaatc acttcagaca tatgtatgtg caggaataga aaatgaccat
     7141 gaatgtataa cttttggtga agtatatgaa aaattgagag caggattaaa aattttaata
     7201 agagaaggaa gcgcagcaaa aaatttaaag tctatagtaa gtggtatgct aaagcataac
     7261 ttgccaattg aagaatttat gttttgcaca gatgataagc acttagatga tatagaaaaa
     7321 caaggtcaca taagatggaa tataaaatgt gcgattgatt taggtatgga acctgttagg
     7381 gctataaaag ttgcaactta taattctgca aaagcatatg ggttaaggaa aattggagca
     7441 ataggtgcgg gttataaggc agatattgtt gtattgaatg atttagacaa aatggaagtg
     7501 gatagtgtat ataaagatgg taatttggta aacgaagaga tgttttctaa ttacaattat
     7561 gagattaaag ataaagaatt gcttaataca gtcaagttta aatatattaa taaagaaaaa
     7621 atacagttaa aagtaagtga aaaaaattat gtaatggaga ttgtacctta tcaaattttg
     7681 acaaataagt tatatgaatc tttaccatgt gcagatggat attttgttcc aaataaagag
     7741 tattcaaaac tttgtgttgt tgagagacat agaatgactg gaaatgtagc aattgctcca
     7801 ctaaaaggat ttggcataaa aaatggagca attgctacta ctgtggccca tgattctcat
     7861 aatattatag ttgcagggga taatgatgat gatattctag tagctataaa ttacttaaag
     7921 gaaatacaag gtggttatgt aattgtatca aatggaaaag ttcttgcaca cctaagtctt
     7981 caagtagctg gattaattag tacttttaca gctgaagaag tacaagaaat tactgataat
     8041 atgttggaga tagcaagaaa aatgggagtt ccagagtatg tagacccttt tattacactt
     8101 tcttttatgg cacttccagt tgttccacaa ataagactta cagatttagg tctatttgat
     8161 gttgaggaat ttaaatttat atagataata taaaagtaat aataaatgag gctttctcaa
     8221 aatagggata agtctctttt tattgttgaa atgatgaaaa tatatttgat ttcatacaaa
     8281 aaagagcatc tcatgaacta aaaattttat ttttagatat ccttatttta attaatctat
     8341 catttaacct tgttttaagc atagatatct taataaaact attttcagtc tattttaaca
     8401 tataaataca tgaaaattat atatttatga aactttatcc ataaaaaata aaatatttta
     8461 tttatatata gatttatagg gtaataatat aagcatagta taaaattatt aataaaaaca
     8521 aaaggagaga tatttatgtt gagtatagga acaaaagcac cagaatttac actagaagat
     8581 aaagatggaa ataaggtaag tatgtcagat tttaaaggta aaaaagtagt agtatatttt
     8641 tatcctaaag ataatacacc aggatgtaca cgtcaagctt gtgcttttag aaatgcatat
     8701 gatggtttta aaaaggaaga tatacaagtt attgggataa gtaaagatag tataaaatca
     8761 catcaaaaat ttgcagaaaa gcatgaactt ccttttattt tactttctga ccctgactta
     8821 gttgctataa aagcttttga tgtatggaaa gagaaaaaaa tgtatggaaa aactgcattg
     8881 ggagttgtac gtgcgacata tattatagat gaaaatggaa ttattgaaaa agtctttgaa
     8941 aaagcaaaac cagacacaaa tgcacaagag attcttgaat atttggaaaa acaggagtaa
     9001 taagaattta tgattataat gttatcacca gctaaaaata tgaagaatat agaagtattt
     9061 gatagagatt tgagtttgcc ttgttttata gataacacaa aagaaatagt ggaaaatata
     9121 aagacatttg ctatagaaga ttttaaaaat aaaatgaaaa ttaatgaaaa attagctgta
     9181 ttaaataaaa atagatttga aagcattaaa tttgatagat taggaaaccc tgcaatctta
     9241 acttatgatg gaatacaata taaaaatata gaagcagaaa attttacaag gaaagatgaa
     9301 gagtttgcaa atagctgtat tcgaatcata agtggattgt atggtgttgt aaaaccatat
     9361 gatagtatat atgaatatag attagaaatg cagacaaaat tacgagtagg tgagtttaag
     9421 aatttatacg aatattgggg aaatcgtata tataaagaac taatcaaaga aaaaacagct
     9481 atcgttaatc tttcatcaaa tgaatacagc aaatccatag aaaaatttat taaagatagt
     9541 gatacatata ttacatgtac ttttaaagta aataaaaatg gcatattgaa agtagaatct
     9601 acacaagcaa agaaagctag aggtatgatg acaaaataca ttgtaaaaaa taggataaga
     9661 gatattgaag aactgaaaaa gtttaatttg gaaggttata agtataaaga aaacttatct
     9721 aacaatagtg aatacatatt tgtaaaagag tagaagtttt gttaaatatt aaatggcatt
     9781 gttaaattta ggtaaaatat acttagtgtg tattgaattg aacttgattt taaaatataa
     9841 ttattcttaa attaaaaaaa gaaaggagtg gtcttccaaa atggaaaatc acaaacagat
     9901 gccttggcat tcaagagcaa cgcatgaaat tttagaaagt ctagattcat cagaggaggg
     9961 gctcaccgat accgaggcgg aaaaacggct taaagagaat ggtttaaatg aacttagaaa
    10021 aaaaccacct agaacaactt tacaaatgct ttggagtcaa attacagatg ctatggttat
    10081 gatactaata ggagcagcaa tattatcttt gatatttggc gaatttacag aagcttttgt
    10141 aatactaata attgtaacag taaatgcagt tattggaatt gttcaagaga aaaaagcaga
    10201 atcatcatta gaagcattaa aaaatatgaa ctcacctaca gctcgtgtta tgcgtgatgg
    10261 tgaagaaaat gttattccag caagtaatct tgtagcagga gatattgttt ttttagaaga
    10321 tggagctatg gttccagcag accttcgttt aatagaaaca tcaagtttaa aaattcaaga
    10381 ggcttcttta acaggagaat ctgttccatc agaaaaagat gcagatacaa tcttacctaa
    10441 agaatgtgta ttaggagacc gtagcaatat ggcgtataca tcttcaatag tcacatatgg
    10501 tagaggtgta ggtgttgtag tagcaactgg tatggataca gaagtgggta acatagcaca
    10561 tcttttagat tcacaagatg attttgatac tcctataaaa cgtaaactta atactgttgg
    10621 aaagacatta agtgtcatag gtattattgt atgcgttgtt atatttgcaa taggtgcatt
    10681 ttatcagaga cctctcttgc caatgtttat gattgcaatt tcccttgcca tatcaattat
    10741 tccagaggga ttacctgcaa cagctactat tgtaatggct ttaggcgtac agcgtatggc
    10801 taaaagaaat gctttaatta gaaaattacc tgctgttgaa acattaggag gagctacagt
    10861 aatatgttgt gataaaactg gtacgttaac cttaaataaa atgactgtta cacatattgc
    10921 tgtcaatgga gattttgaat ctggaaaagt gacatcaata gatactgcat ctgaaaaaca
    10981 tccattagtg tataaagaga ttgtttatgc tggtgcactt tgtaatgatg catgtattaa
    11041 tccagataaa acagatgaaa tattaggtga cccaacagag ggcgcattaa tttttatggc
    11101 caaaaaattt ggaatagatc aagaagctct tgaagaagaa tatccacgtg tatttgaaca
    11161 gccttttgat tcagatagaa aatgtatgac aactgttcat aatatagatg aaaagattat
    11221 tgcttatact aaaggagcag tagacgaaat tcttgaccta tgtactaaaa tattaacttc
    11281 aaaaggtgaa agaaacatta ctgaagtgga taaaaagaat atacacgaat tatgtcttaa
    11341 catgtctaaa gatgcattgc gtgtacttgg ttttgctaag cgtgagattt catctatacc
    11401 aaaagaagat agtgaaaata tagaatatga tttaacattt ataggtatgg ttggtatgat
    11461 tgacccacca agaactgagg taattgatgc agtaagtact tgtaaagaag caggtataag
    11521 aaccataatg ataacaggtg accataaagt tactgccaca acaatcgctc atgagttagg
    11581 aatatggagt gaagaaaata ctgtgatatc tggtgatgaa cttgataatt tgagtgatga
    11641 tgaactagat gaagctgtta aaaatactac agtatttgct cgtgtatccc catttgataa
    11701 acttagaatt atacaatctc taaaaagaat tggagaagta ccagcaatga ctggggatgg
    11761 tgtaaatgac tctcctgcat taaagtctgc tgatattggt atagccatgg gtataagtgg
    11821 tacagatgtt gcaaaagatt cttctgacat gattttaatg gacgatagtt ttacaacaat
    11881 tgcttatgca ataaaagaag gtagacgtgt atatagaaac atacaaaaag ttatacaatt
    11941 tttgcttgtt ggtaatatag cagagatact tacgttgttt gtagcaatat tattaaattg
    12001 ggatactccg cttcttgctg ttcatattct atgggtaaac cttgcaactg ctacgcttcc
    12061 agcccttgct cttggagttg acccaccaag cagaaatata atgaagcacc agccagtgaa
    12121 atcaaccact ttgtttgaaa aaaatcttat acaaagagtt gttacccaag gtatatttgt
    12181 ggcaataatg actattttag catataaaat tggtcttaat atagaaaatc attctgtagg
    12241 tcaaacaatg gcattttgtg tattagcatt ttcacagatg ttacgagcat ttagtcagcg
    12301 ttcaaataca gattcaatgt ttaatagaaa taatggtaaa aatccatttc ttttaatctc
    12361 atttttaact tctgcatttt taatagcagt aatactgttt gtacctgttt ttagaaatgc
    12421 ttttaactta acagttttaa ataacttaga gtggcttata acgattgttt tagccataat
    12481 gtctgtaatt caagtagagg ttgggaaaat tattaaaaga aaaaaattaa aaaaatagtt
    12541 gacaaaatta ggaaaaatat ataagatata aaacatagaa aaattaaata aatcttatcc
    12601 agagaggtgg agggactggc cctatgaaac ccggcaacca gtatatttat atacattggt
    12661 gctaaatcct gcggcgaaag ctgagagatg agtataatta agcttataca gagcctgagt
    12721 tatatactca ggctttattt attttaactc tcatctggta aatagtttaa ttttttttac
    12781 taaaatctaa gggggataca agaatgtata ataaagaaac aatatgtgtg caaggaaatt
    12841 ataaaccagg taatggagaa ccaagagtac tacctttata tcaaagtaca acttttaaat
    12901 atagcagtat agaccaactt gctgaattat ttgatttaaa agttgatgga catatatatt
    12961 caagaataag caatcctact attcaagctt ttgaagaaaa aataagttta ctagagggtg
    13021 gagtatcttc tgtagctgta tcatcagggc aatctgcaaa tatgttggca gttttaaata
    13081 tatgtaaatc aggagatagt atactttgtt cttcaaaagt atatggagga acattcaatt
    13141 tactaggacc tagtcttaaa aaatttggta tagatttaat atcgtttgac ttagattcaa
    13201 gtgaagatga gatagtagaa cttgcaaagg aaaatactaa ggttgtgttt gcagaaacac
    13261 ttgcaaatcc aactcttgaa gtcatagatt ttgaaaaaat agcaaatgta gctaagagaa
    13321 ttaatgttcc atttattgtt gataattcat tagcatctcc agtgctttgt aaccctttaa
    13381 agtatggagc aaatatagtt actcattcta ccacaaaata tttagatggg catgcttcaa
    13441 gtgttggagg aattatagtg gatggtggaa actttaactg ggataatgga aaatttccag
    13501 aattagttga gccagaccca acatatcatg gtataagcta tactcaaaaa tttggaaatg
    13561 ccgcatatgc aactaaagca agagttcagt tgcttagaga ctatggaaat tgtttaagcc
    13621 cattcaatgc gtatcttact aatttaaatg ttgaaacact acatcttaga atggagagac
    13681 atagtgaaaa tgcacttaaa atagctagat ttttagaaaa acatgaaaat gtagattgga
    13741 ttaattaccc aggacttgaa gataacaagt attatgagaa tgccaaaaag tatttatcaa
    13801 gaggatgtag tggtgtttta tcatttggag taagaggtgg gttagaaaat gccaaaaaat
    13861 ttgtggaaaa attacagata gcatctttgg ttacacatgt ttcagatgta agaacttgtg
    13921 ttatacatcc agcttcaact actcatagac aattaacaga ggaacaatta attgcatctg
    13981 gagtattgcc ttcactaata agattatctg ttggaataga aaatgtagag gatttaatag
    14041 ctgatttaaa tcaagcttta aatttctaat attctaagag aaagagaagt gaaaagatat
    14101 ggctttaata ctaccaaagg gattacctgt tattaataaa ttattagatg aaggaataga
    14161 tgtaatttat aaagaagatt ttaaaaagga attaaagtat gaagaaaaca tagacactaa
    14221 gatagctata ctaaatctaa tgcctataaa gatagataca gaattagatt tactaagaag
    14281 gatagataaa acaggattca acgtgtctgt ggaatttatt aaaatttcaa caagagaaag
    14341 taaaagaagt tgtaatgaat atgttaaaga attttataaa acatttgatg aagccaaagg
    14401 agagtatttt gatggcttta taataacagg agctccagtt gaacaaatgg agtttgaaga
    14461 agttgattat tggaatgaac tagaagaaat tatggattat tctaagagaa aaactaaatc
    14521 tacattatat atatgttggg cagctcaagc aagtctttac aaatactaca atgtaaaaaa
    14581 acttccacta agtcaaaaat gttttggagt atttaagcat aaggtagata aagatagtaa
    14641 aatagtagat ggatttgaaa atgaattttt tgctccacat tcaagacata ctactgtaaa
    14701 tattgaagct ttgaaagaga ataaagagtt gagcattgtg agtcattcta aagaagctgg
    14761 tccctatata attacaaatt caagagatgt gttcgtaatg ggtcatagcg agtatgacaa
    14821 atacactctt gataaagaat ataagaggga tataaataaa ggagataaaa tatctatacc
    14881 tcaaaattac tatattaatg atgacccttc agaagagcca actgtaaaat ggaaaaaaca
    14941 ttcagaattg ttgtttagaa attggataaa aaactatttg atacaataaa ttttaagagt
    15001 atataaatgt taactaaaaa aatatagaaa tttataggta taaagttaat cttaatgata
    15061 ctttatacct tattttttta aaatttgacc ttataatagt agttttatat tttaagaaaa
    15121 tcactttagg tgtatgtaga tagaggtggc tttaatttta tatatggtgc gataatggga
    15181 gactaaacct ttatagtatc aataaatttg tattttaact tttaaaagag attaaaaagg
    15241 gagaaatcag tgtaaaatat atatatagat tttttattaa attttattaa aaatatcctg
    15301 tataaaatta aatatcaata agataacaaa atttcaattg aagttacaat attgtaattg
    15361 acaataaata cttaatacta taatatttaa tacattaaac attatagtgt taagtattta
    15421 tttacagtat gcacttcagc ctaaatttga aataaagtat tttcgtaggg agcgtttgta
    15481 tggatgagat gagttttaag aaagagctac tagagttgac tagagatata aatatgaaat
    15541 ttacaacttt attaagtgat ttttatcagc cacttggtat tacagcagtg caagctttga
    15601 ttttatctga gttatgtgaa catggagaga aaaaaataag cgatttaggg aagaacttaa
    15661 acatgacaaa tagtaatgta tcagtaatct gtcaaaggct tgaaaaaaat ggctttttaa
    15721 atcgcataag agatatagaa gaccaacgaa ttgtaaaagt aaaagttaca aataaatcat
    15781 tggatataca agaacatatt tcaagtagta tttttgatag ttattttgaa aatatgacca
    15841 gtgaaaaatt gcaagatatg gaagatataa tagaaggttt agagaaatta aataaacttt
    15901 taacttacat tgattgcaaa taacataaaa ataatttaaa ggagaataaa attatggatg
    15961 aacataaacc ccaaaaaagg tcagcactct attattatac agttataata atagtagtaa
    16021 tgtttttaaa tgctttaata tttccaagtt taatggataa aaaaatggtt gagattgact
    16081 atggaaagtt tctcacaatg ttggatacag gaaaggttaa atctgttgag atagaaaata
    16141 atagaatagc aataaatcca tctagtagca aggataagaa tatatacata actggtagaa
    16201 tggatgaccc agaattggta gatagactaa aaaatgcaaa agtagaattt actaaggtta
    16261 ttcctaagga aaattcacct cttttaagta ttttacttac atggataata cctataggta
    16321 ttcttatgct atttggtaac ttaatgatga agtctatgca aaagcgaatg ggtggtaatg
    16381 ctatgcaatt tggaaaaagc aacgccaaaa tgtatgtatc tgctcaaagt ggaaaaagat
    16441 ttaatgatgt agctggtcaa gatgaggcaa aagaagcttt aactgaaata gtagatttct
    16501 tacacaatcc agaaaagtat aaaaagatag gagcacaaat gcctaaagga gcacttttag
    16561 taggacctcc aggtacagga aaaacactcc ttgcaaaagc tgtagcaggt gaagcaaatg
    16621 taccattttt ctcaatatct ggttctgagt ttgttgagat gtttgtagga atgggagctt
    16681 ctaggattcg tgatttattt aaacaagcaa aagaaaaagc tccatgtata gtgtttatag
    16741 atgagattga tactattggt aaaaagcgtg ataatggaag tggattaggt ggaaatgatg
    16801 aacgtgaaca aactttaaat cagttgttaa gtgagatgga tggttttgat ggtggaattg
    16861 gagtagtaat acttgctgct acaaatagac ctgacagtct tgacaaagca ttacttcgtc
    16921 caggaagatt tgatagacgt ataccagttg aacttccaga ccttggagga cgtgaagcta
    16981 tattaaaggt acatgctcaa aatgtaaatg taagctcaga tatagattat aatgcaattg
    17041 caaggtctac atcaggagca tcaggtgcag aacttgcaaa tattgtaaac gaagcagcac
    17101 ttttagcagt taagtctggc agagatatag tagaacaaca ggattttgat gaatctgttg
    17161 aaactgtaat tgctgggtat caacgtaaag gagctgtaat ttcagataag gaaaagaaaa
    17221 ttatagcata tcatgaaata ggtcatgcac ttgttgctgc tatgcaaaaa cattctgcac
    17281 ctgtccataa gattactata atacctagaa cttcaggagc attgggatat actatgcaag
    17341 ttgctgaaga tgagagtgta cttatgtcta aagaagaagc aattgataaa ataactactt
    17401 ttacaggtgg tcgtgctgct gaagaggtta tctttaacac ttgtacatca ggagcatcaa
    17461 atgatataga acaagctact aaaattgcaa gagctatggt tactcgcctt ggtatgagtg
    17521 aagagtttga tatggtcgct cttgaaactg taagcaatca atatttagga ggagatactt
    17581 ctcttgcttg ttcttccgac actgctgcta aaatagatga agaggtattt aaaatagtaa
    17641 aggcttgtca caaaaatgca attgaaatct tagaaaataa tattgataaa ttgcatgaac
    17701 ttgctaatta tttattagaa aaagaaacta ttacaggtga tgagtttatg gaaatactta
    17761 acttaaacta aaatgattat acacaagata aaaatgccta ttgttaggca tttttatttt
    17821 acaactaaat tttttaaatt catatttaaa tctttacata atcttaatca ttaaaaatac
    17881 atcttaacta caattttata tcggaattga tattataata gtaatgaatg gtgtaaggag
    17941 gttttttaat ggatagacct aatcctgata ttctattaga aaaaattaaa aatgaagaag
    18001 aaaaactaag tagaggtcaa ttaaaaatat tctttggtta tgcagctgga gtaggaaaaa
    18061 cctactctat gttggaatct gcacaaaatt taaaaaaagt gggtgtagat gtagtagttg
    18121 gatatattga accacacact agaccagaaa cattggcttt acttgatggc ttggaaattc
    18181 ttcctgtaaa ggatatagaa tataaatcta taaaactaaa agaatttgat ttagatgctg
    18241 ctttagcaag aaaaccagaa gttatattag tagatgaatt tgcccattct aatgttgctg
    18301 ggttaaggca tacaaaaaga tggcaagata tagaagaatt gctgttagca ggcataaatg
    18361 tctacactac tgtaaatgta caacatttag aaagtctaaa tgacattgtt gagataatta
    18421 caaacgtatc agttaaagag actataccag ataagttttt ggatacaaat acacagttag
    18481 aacttataga tgttgaacct gatgtacttc ttgaaaggtt caatgaaggt aagatatata
    18541 agaaagaaca agctactaga gcaaaaaata atttttttat aaaagataac ctggttgcac
    18601 ttagagaaat tgcacttaga aaaacagcag agagagttaa taaagaagtt caaatgacca
    18661 gactatcaaa gggtgatgta actgtaatac ctacatcaga tacacttctt gcatgtataa
    18721 gtccatctcc atcttcagca aaagttatta gaactgcatc cagaatatca gacagtacat
    18781 ttgcaaaatg gatagcttta tatgttgaaa ctccaaacac agcaagactt ccatttgatg
    18841 aacaaaaaca actacaaaat aacctaaaat tagctaagaa attaggtgga gaaataattg
    18901 ttttacatgg tgaaaatata atagagcaaa tacttagaat tgcaaaactg agaaatgtaa
    18961 caaaaattgt aataggtaga aatcattcaa gtaataaaag atttagtaaa agatttaaaa
    19021 aagatatagt tgacaaatta attgatgaag ttgattatat agatatacat ataattccat
    19081 ataaatttgc ctcagatgtt aaatacaaac ctaaaaagga taaatcatca ataaaatcta
    19141 aatttaaaat tagcaaagtt gattttataa aacttatatt tattactttg atgataagta
    19201 ttttagcata tgcagtacaa agcatgggat ttataagaga aaatatattg ttaatatata
    19261 tgttgggtgt agttttagtt tctatgtgga ctaaagggta tagtacaggt ataataagtt
    19321 ctgtatttaa tatagttctt ttaaattatt tttttacagc accattatat acattatcta
    19381 tagcagattc aaactatata gtaacactgg ttgtattctc aatagtaggt ataataacta
    19441 gcactcttac gtctaagata cagcatgaag cagaaactgc tgctaaaaga gaagaaaata
    19501 caaaaatgat atatcaaata attaagggat ttttaagatt atcaaataaa gatgatatag
    19561 taaacaaagg tatagaactt ttgtctctta gcttaagtag agatgtaatt tgttatttaa
    19621 atgtagataa gcataataaa tcaaagcttt acaaaaaaaa tactaataat aaggataaaa
    19681 atgatttgga tagtgaagat gaaaaagctg ttgcaagttg ggttttatcc aattcaacag
    19741 tggcaggaaa tgacacggat acacttcctg gttcaaaggg atactatata ccaattgtag
    19801 gaatgaattt aactttagga gtagtgggaa tatcatgtat tgattcaaaa ttggatacag
    19861 aagatataag cttaattgaa acaataattg ctcaaatggc aatagcgctt gatagagaaa
    19921 tattgtcaga agctaaagaa aataccaatc ttgagattga aagggaaaga ttaagaagta
    19981 atcttttaag agcagtttcg catgacctta gaactccact agcaggaata tctggtgcag
    20041 taagtacaat tataaaaaac aagggtacta taggtcaaga tataatagat gaattactta
    20101 gtggtgtata tgaagatact cagtggttaa taagacttgt tgaaaattta cttagtatga
    20161 ccaagataga tgaagggaaa ttggaagtta aaaaacattc tgaactggtt gaggaaatta
    20221 tatcagaagc gttacaaaaa ataaaaaaaa gaattgaaaa tgcacttata gatattaata
    20281 taccagaaca gatattattt gttcctatgg atgcaaaatt aatagaacaa gttttaatta
    20341 atttaataga taattctttg aaatattcaa aggaagattg taagatagag ataaatgtat
    20401 atgagaaaga tgattatgtt tggtttgaag tatctgataa tggtcctggt atatcaaagg
    20461 aacttaaaaa gcatatcttt gacagatttt ttactggaga agaaggagct aaagattcca
    20521 gaaagggtgt tggacttgga ttatcaattt gtaaatctat aattcaagct cataaaggtg
    20581 aaattatggt agaaaacaat aaagataagg gttcaacttt taagtttagt ttgcctaagg
    20641 aaaatgaata gttttaaatt ttaaaattag ttttggagga agaaatgagt aaaaaactta
    20701 tattgttagt agaggatgat aagacaataa gaaaatttat atctactgca cttttaacac
    20761 aggattatga tgtcaaggag gctataactg gaaaagaagg tatatctatt gctgtctcat
    20821 attctccaga tgttgttctt ttagatttag gcttagaaga tatggatgga atagaggtaa
    20881 taaaagctat cagacaattt tcaaatatac caataattgt agtttcagct agagagcaag
    20941 atagagacaa ggtagaagta tttgatgcag gggcagatga ttacttaaca aaaccatttt
    21001 ccatagtaga gttattagca agagtaagag tagcatttag acattcacaa gtagaagctc
    21061 aacaaaaaga tgatgttaaa tctacgtttg aagtagataa attattgatt gattttgata
    21121 aaagaaaagt tattgttgat gatgttgaag tccatttgac acctattgaa tataatattc
    21181 tttcattact tgcaaagcat catggaaaag tgcttactca taattttata ataaaagaaa
    21241 tatggggtag tgttataggg aatgagacta agtctttaag ggtttttatg gcaacgttaa
    21301 gacgaaaaat agaaaaacaa cctgcaaacc caagatatat aattacagaa gttggtgtag
    21361 gatacagatt aaatgatgaa tagttgtaat atattaatta ttagattatg tgcttattaa
    21421 tatatattaa aattatatag tttagttaaa ataagaatga agtgtatgtg cttgttgtca
    21481 gtttagcaca tgcattttat tttttagctt ttttagtatc aataattttt tagtatcaat
    21541 aatatgaaat atgttttaaa ataagtatat agtgtaatct gtaataaaaa ctttggttaa
    21601 ataataaatg tatttaacac ttaccttata ataaatttaa tataaatatt aaataataat
    21661 tattaaaata gtgtaaaata attacagtaa tgattataaa aaatttttag aatgtaaatt
    21721 gaaatttagg agtagatgta tgtatatact gaatttaaat acaagagaga ctattgaaga
    21781 ttttagagat aaattttatg tagcagaaaa ttcttatctt atattaagtg ctccaaaaaa
    21841 tttaaaattg cttaaggaaa ctttggatat agatgaaata acttttaatg attgtcttaa
    21901 atttgatgaa ataaccaaac ttgatttatt tgataattac gattttttaa gcctaaatac
    21961 atttgaacta agagatggag aagctgttat agaagaggta aatatgtatt tatcagataa
    22021 ttttatattg gttgtagtga atgaagaaca ctttttattt gaatttgtaa aaaatattat
    22081 tttgaagaac tctcaacttg aaaaaaatcc agttattaat ctatttaaga taaactactt
    22141 gattcttaga gaagttataa aaaatggttt tgagtctttg gaaaaagttg aagagttaat
    22201 tcttcaaata gaagatgaaa tgatggacaa cattaataag aatcatgtat ctagaataag
    22261 tgatgtaaga ggattaacaa gaattatagt taagaatact agacctcttt tgtatattgg
    22321 ggatagaata gttaaggaaa atataagata tttaaaatat tcaaatgtaa aaaaatataa
    22381 tttagaaaat tttcaaggta tagattttgg aattgacaaa ttatacaatt ttgcattgtc
    22441 aacacgagag ttagcagata aattattaga tatttattct tccagagtag gtgagaaaac
    22501 aaataactta attacaaaat taaccttgtt aacagccata tcagcaccac ttacaattat
    22561 aactggtata tatggaatga actttagata tatgccagaa ttaaactgga tttatggata
    22621 tccagcaact ttatttttta tgctatgtat aatttttgtt ggaattataa tatttaaaat
    22681 aaaaaaatta ttgtaaatta cagatgatgt ttaattgatt ttataaaaat acatcaattg
    22741 agaagctgat ttaagtaaat aaaaaaatat tttaaattga aaatatttat agaaaatat
//
LOCUS       DAELJO010000047        22484 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELJO010000047 DAELJO010000000
VERSION     DAELJO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.047"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            863..1417
                     /locus_tag="KPB88_001540"
                     /pseudo
     CDS             863..1417
                     /locus_tag="KPB88_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1612..2016)
                     /locus_tag="KPB88_001541"
     CDS             complement(1612..2016)
                     /locus_tag="KPB88_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0053824.1"
                     /translation="MLIAEFAENEEAVFRQTIEWLTSNLANTPTLIRKQASGFNIDTF
                     TRTFTNYKGENVYLTAKEFDLLYFFICHKGQVFTKEQLYRNVWGYDNAPLGSNLTSFI
                     RKLRKKIEPSPDNPQYILTVWGVGYKFNKEKP"
     gene            complement(2059..2460)
                     /locus_tag="KPB88_001542"
     CDS             complement(2059..2460)
                     /locus_tag="KPB88_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HBG0053825.1"
                     /translation="MDKKKVAFICVHNSCRSQIAEALAKHFASDVIESYSAGTVKKPQ
                     INQTAVRIMKQIYGIDMEKDQYSKLIDDIPTPDIAISMGCDVGCPYIGKAFDDNWGLI
                     DPTGQSDEVFKTVIAQIEKNILLLRSNLVRG"
     gene            complement(2453..3496)
                     /gene="arsB"
                     /locus_tag="KPB88_001543"
     CDS             complement(2453..3496)
                     /gene="arsB"
                     /locus_tag="KPB88_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HBG0053826.1"
                     /translation="MAREKGINVFQRYLTVWVAICMIVGVLISKYLPTIPEFLNKFEY
                     AKVSIPMALLIWLMIYPMMMKVDFKSIKDVRKNPKGLFVTWITNWLIKPFTMYGIASL
                     FFFVIFKSLITPELATEYLAGAVLLGAAPCTAMVFVWSSLTKGNPAYTVVQVATNDLI
                     ILVAFVPIVKFLLGVSNVSVPWDTLILSVLLFVVIPLMGGMATRHYVIKKKDKEYFES
                     TFLHKFDNVTSIGLLLTLVMIFSFQGKVFLENPLHIILIAIPLILQTFLIFAVAYVSC
                     RLLKLPYNISAPAGMIGASNFFELSVAVAIALFGTTSPVALATTVGVLTEVPVMLFLV
                     KIANNTKGWFSNG"
     gene            complement(3508..3882)
                     /locus_tag="KPB88_001544"
     CDS             complement(3508..3882)
                     /locus_tag="KPB88_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBG0053827.1"
                     /translation="MSLFNLGKKKEETKKPACACNGACSTSEATEISNTCCNEASDGI
                     CCIKVLGSGCKSCHALLESTKEAVEAMELSIEVEYVTDMQKIMEYGVMSMPALVVNEN
                     VVSMGKVLKASDVENLLHKLGF"
     gene            complement(3913..4926)
                     /locus_tag="KPB88_001545"
     CDS             complement(3913..4926)
                     /locus_tag="KPB88_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0053828.1"
                     /translation="METLELGWDFFQNEILGMGWLNRLIGALLNTCGLDTSSRLGGSI
                     QFFMYDTIKIMVLLGVLILIISYIQSYFPPERTKKILGRFDGIGANIIAALLGTVTPF
                     CSCSSIPLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGWKVAVVYVVLGLV
                     IAVVGGTLIEKMHLENQVEDFIRKGNAIDVPQEELQFNDRMKFAWEQVVETAKKVAPY
                     VLIGVGIGAFIHNWIPEDIIVKALGTGNPFGVIIATIAGVPMYADIFGTIPIAEALLA
                     KGAMLGVVLSFMMGVTTLSLPSMIMLKKAVKPKLLGVFITICTIGIIIVGYFFNVFQY
                     LLV"
     gene            complement(5015..5329)
                     /locus_tag="KPB88_001546"
     CDS             complement(5015..5329)
                     /locus_tag="KPB88_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0053829.1"
                     /translation="MDLDEKKTAIIFKAFCDENRIKILKLLVTGEKCACKLLEEINVT
                     QPTLSHHMKILCDSGVVTGRKDGKWTYYSISNDGAVYALKCLQELTNTEDTNQERSCC
                     EK"
     gene            complement(5454..5696)
                     /locus_tag="KPB88_001547"
     CDS             complement(5454..5696)
                     /locus_tag="KPB88_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053830.1"
                     /translation="MTYSEAIIKRLTQLCEERKITINRLATLSGITQSTVENLMKGKT
                     KNPKLKTLHKLAIGLDMTVSELLDFPEMNESAFDDE"
     gene            5831..6466
                     /locus_tag="KPB88_001548"
     CDS             5831..6466
                     /locus_tag="KPB88_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053831.1"
                     /translation="MYEISDMLKRSKFSNILSYLTYGTGENEEMVESYDCAIDESYET
                     FFNDIENLYTDADREDNKLFDIITEFACIHDDIYFEAGMLVGFQLYKELTEKYEYHAD
                     KDITAILEKRKQPLDTEHEANSALESFCMHRLNTALEESIRKDKNYQKKDEEIEKAIK
                     RIDKIGLTHNQWKTVDRALSACNARSSGYGEKAYMQGFKDVINLIIEAMDQ"
     gene            complement(6622..7263)
                     /locus_tag="KPB88_001549"
     CDS             complement(6622..7263)
                     /locus_tag="KPB88_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053832.1"
                     /translation="MKYLLLGENIQRVRKFRGMNQQELADSIGINMQSLSKIERGVNF
                     PTFDTLEKITCALGVTPNELLSGEWKNTSHMEQEILKFLEREERLNVELAHGQYDNYF
                     DSEEEWKEYELKKLREYIMDFIGSEDRQASDLYPIKQLIQRHKFQNLLDKYDDLYSLD
                     LFGETIEGHRNINPYEWNPIEGKIILNRHVDGDRESLSSVPTIEGFEDLKGIE"
     gene            7671..8774
                     /locus_tag="KPB88_001550"
     CDS             7671..8774
                     /locus_tag="KPB88_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0053833.1"
                     /translation="MVQRKERLFIAKQEFLTIKIDYISIVFDTATAEEVIRRVLELPV
                     DIFDIQSAKVQHKSYQSVYAIGSIKVFGNAKKNEDNPMGLGSYLVLSGMGCDEIFRIL
                     DMHGQSFGDLFKRCERWFCGKFHFTRLDIAIDDRNEVPYFTIKQLKRKCEREEYIANS
                     NTHRFNESKYEDYSTAKTVYIGAGKSGMSYRFYDKDKEVSMKHDKAIDDIGSWKRTEM
                     QLRDEKAHLFAMLFKDNPFDLGKLAFDLLAGSLRFVVPDKTQSNKSRWKTCRFWKRFL
                     GAVEPLKLHAETPHNTLLETQRWLKEGGVLSAVKAFHFLEVNQALGNLERVEDMLKQV
                     RYSAKLGNKLTGHLCRIHREELIPRVQYDTKKG"
     gene            8799..8993
                     /locus_tag="KPB88_001551"
     CDS             8799..8993
                     /locus_tag="KPB88_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0053834.1"
                     /translation="MDIPVWERYTLTIEEASQYFRIGENKLRKLAEENKHAGWLIRNG
                     NRIQIKRKQFEKIIDKLDAI"
     gene            9072..10262
                     /locus_tag="KPB88_001552"
     CDS             9072..10262
                     /locus_tag="KPB88_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0053835.1"
                     /translation="MSEKRRDNKGRILKTGESQRTDGRYLYKYIDTFGEPQSVYSWKL
                     VATDRVPKGKRDCVSLREKEAEIRKDLEDGIDAVGKKMTLCQLYAKQNAQRGNVKKTT
                     QKQREQLMEILKADKLGARSIDSIKPSDAKEWAVRMKENGYAYQTINNYKRSLKASFY
                     TAIEDDCVRKNPFNFALNTVIEDDRKPRVALTEEQEEKLLSFVESDRTYRKYYDVIIL
                     LLKTGLRISELCGLTVSDLDFENRLINVDHQLLRNKEVGYYIETPKTKSGVRQVPMSE
                     ETCQTLQKVVAERKNIQPIVIDGYRDFVFLNQKGYPMTGAYYTSTFGNLVKKYNKSHE
                     DALPNITPHILRHTFCTRLANKNMNPKSLQYIMGHSNINITLNLYAHASLDGVKAEMV
                     SLIA"
     gene            10552..13059
                     /locus_tag="KPB88_001553"
     CDS             10552..13059
                     /locus_tag="KPB88_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053836.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSVIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            13390..13734
                     /locus_tag="KPB88_001554"
     CDS             13390..13734
                     /locus_tag="KPB88_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053837.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKQLTVTGNEYAIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            13972..14658
                     /locus_tag="KPB88_001555"
     CDS             13972..14658
                     /locus_tag="KPB88_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053838.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            14669..15910
                     /locus_tag="KPB88_001556"
     CDS             14669..15910
                     /locus_tag="KPB88_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053839.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            15981..16664
                     /locus_tag="KPB88_001557"
     CDS             15981..16664
                     /locus_tag="KPB88_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053840.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFQRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            16661..18994
                     /locus_tag="KPB88_001558"
     CDS             16661..18994
                     /locus_tag="KPB88_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0053841.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(19102..19722)
                     /locus_tag="KPB88_001559"
                     /pseudo
     CDS             complement(19102..19722)
                     /locus_tag="KPB88_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(19779..21128)
                     /locus_tag="KPB88_001560"
     CDS             complement(19779..21128)
                     /locus_tag="KPB88_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0053842.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAATLVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFANIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQVVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYDAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(21165..22385)
                     /locus_tag="KPB88_001561"
     CDS             complement(21165..22385)
                     /locus_tag="KPB88_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0053843.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMACILKENADKTICIRADMDALPIEEEN
                     DIPYKSIYSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         8267 a         3294 c         3720 g         7203 t
ORIGIN      
        1 ttaaaaatat aatgaaagtt tagttttaat tcatttattt aataaacatt tcaagtaaaa
       61 taatgtttaa atcagaaata taaataaata ttattataat cgttattaaa gaccaaaaat
      121 cccatcttag aagtttgtat catatgaaac ctttatagat tatgaaagca ataatatagt
      181 gtatgatata taaaataagt tataaaagta tttttttaga tattcaaaaa gacattgtaa
      241 gttatattaa taatatgggt atcaacgaat tttggatata atgagttttt caacttaaag
      301 ttatgtaata atgaatgtaa agaatattta atgaaatcat tttgttggtt atgtctagct
      361 gaatacaata atcttttaga taaagatttt atatttgaag ataggtgtga aaaaattaag
      421 aagatattaa catcagcagt ttagaggcaa agcatgtaaa gtaaagtctg ggtaactaaa
      481 agtagtaatt tagattgtaa ctattgttat aaatatgaaa atgaggaaac aggaaatata
      541 agttaggatg tagaaaatag gttactaaat aaaaagctta attatataat caaagaaata
      601 ggtatatact gtacaatttc atggtgaaga acttttccta aataaaaagg ttggtaaaag
      661 agatataaga aataaaattc aattataaga tatggtatga taactaaagg cactatatgg
      721 agtgaatagg cggaagaatt ttttgaaaat tataaaaata gtttctgttg ctacatatca
      781 ataagtatag atagagattg caaaatccat agcaaaaatc gaatttataa gtgtaaataa
      841 catgtaagac aaggagtaaa ttatgagtat tttagagata aaaaacctaa ataaaatata
      901 tggtaaatcg gaaacagagg tgaaagcgtt aagtcaaata aatctaaaaa taaatagtgg
      961 agaatttgtt gtgatagtag gtaaaagtgg aagtggtaaa agtactttac tacacataat
     1021 ggcaggactt gaaactccaa caaatggtga tgtgattatt gataatataa atatatcatc
     1081 tcttgatgaa aagaaaagat ctgcattacg taaagaaaag ttgggattaa tatttcaatc
     1141 atataatttg attccagttt taactgttga agaaaatata aaactaccaa ctataaatat
     1201 aaaaaataaa gatgaagctt atattaatga attgatggag ttactaggaa taaaagatag
     1261 aagaatgtat ttgccaaatc aattatcagg tgggcaacaa caaagagtag caatagcaag
     1321 ggctcttgta aataaaccat caattgtatt tgctgatgaa cctactggaa atttagacac
     1381 aaagacagag tcagatgtat taagtttatt aaaagaaatt taaaatataa tcactgaata
     1441 tcgcttaaac actaggtttt tcattgtaaa ttaatgtctt aaaggtttaa tttactacta
     1501 atttaccatt tatgaaagag ctttgatatg acaacgtaaa acgatataga aagatggttt
     1561 gtaaaatata aaaaacgtag attatataat cactattaag agatatataa cctacggttt
     1621 ttctttatta aatttgtaac caactcccca aacagttaat atatactgcg gattatctgg
     1681 ggatggctct atcttctttc ttaactttct tataaatgat gtgagattac tccctaatgg
     1741 agcattatca taaccccata catttctata tagttgttct tttgtaaaga cctgtccttt
     1801 atgacagata aagaaatata ataaatcaaa ttcttttgct gttaaatata cgttttcccc
     1861 tttgtaatta gtaaaggttc tggtgaaagt atctatatta aatccagatg cctgctttct
     1921 aataagagtt ggggtattgg caagattaga ggttagccat tcaatagtct gtctaaagac
     1981 tgcttcttca ttttctgcaa attcagctat taaaattttc ccatgctacc acctctttca
     2041 cgtataaatt atctaccgtt atcctctaac tagattactt cttaatagca atatattttt
     2101 ctctatttga gcaatcactg ttttaaatac ttcatcactc tgcccagtag ggtctatcaa
     2161 tccccaatta tcatcaaacg ctttaccaat ataaggacat ccaacatcac atcccataga
     2221 gattgctata tctggtgttg gtatatcatc aattaatttt gagtattggt ctttctccat
     2281 atcaatcccg tatatttgtt tcataattcg tacagcagtt tgatttattt ggggcttttt
     2341 caccgtacca gcagaataac tttcaataac atcagaagca aaatgttttg ccagtgcttc
     2401 ggcaatttga cttcggcatg agttgtgaac acatataaat gcaactttct tcttatccat
     2461 ttgaaaacca acctttcgta ttatttgcaa tttttacaag aaacaacata acaggaactt
     2521 ccgtcagcac tccaacggtt gtagccaatg caacaggtga tgttgtacca aacaatgcaa
     2581 tcgctaccgc aacagaaagt tcaaagaaat ttgaagcacc tatcattcca gcgggagcag
     2641 atatattata gggcagtttt aataatcgac aactaacata agcaactgca aagattaaaa
     2701 atgtttgtaa aataagtggt atcgctatta aaataatatg caatggattt tctaaaaaca
     2761 ccttaccttg aaatgaaaat atcatcacta atgttaataa aagccctatg ctggttacgt
     2821 tgtcgaattt gtgtaaaaag gtactttcaa aatattcctt atccttcttt ttgattacat
     2881 aatgacgggt agccataccg cccataagcg gtataactac aaataaaaga actgatagta
     2941 tcagagtatc ccaaggaaca gaaacatttg aaacaccaag caaaaatttt acaatcggca
     3001 caaaagcaac taggatgatt agatcattcg ttgctacctg tactactgta taagctggat
     3061 tgcctttagt gagtgaactc catacaaata ccatagctgt acagggagct gccccaagta
     3121 aaacagctcc tgcaaggtat tctgtcgcaa gctctggtgt gattaaactt ttgaaaatca
     3181 caaagaaaaa tagacttgca atgccgtaca tggtaaatgg cttaattaac caatttgtaa
     3241 tccaagtaac aaataatcct tttgggtttt tgcgaacatc ctttatactt ttaaagtcta
     3301 ctttcatcat cattggataa atcatcaacc atatcaataa tgccatagga attgaaacct
     3361 ttgcatattc aaacttattt aaaaattctg ggattgtagg aagatatttg cttatcagta
     3421 cacctacaat catacatatt gcaacccata cagtcaaata tctctgaaaa acattgatac
     3481 ctttttctct tgccacataa acacccccta aaagcctaat ttgtgtaata gattttcaac
     3541 atcactggct ttcagtactt ttcccattga aacaacattt tcatttacaa ctagagctgg
     3601 catactcata acaccatact ccattatttt ttgcatatct gttacatact caacttcaat
     3661 cgaaagttcc atagcctcta ctgcttcttt tgtactttca agcaaagcat gacaactttt
     3721 acatccagag ccaagtactt tgatacaaca gataccatca cttgcttcat tacagcaagt
     3781 gttagagatt tcagttgctt cacttgtaga gcaagcgcca ttacaagcac aagctggttt
     3841 ctttgtttct tctttctttt ttccaagatt aaataatgac ataataaagt cctcctttaa
     3901 atttttgtat tattatacga gtaggtattg aaatacattg aaaaagtaac ctacaataat
     3961 aattccaatc gtacaaattg ttatgaaaac tcccaatagt tttggcttaa ctgccttttt
     4021 tagcataatc attgatggta atgagagtgt ggtaacgccc atcataaatg ataatactac
     4081 accaagcatt gcgccttttg caagtaatgc ttctgctatc ggaatagttc caaatatatc
     4141 ggcatacata ggtacgcctg ctatcgttgc gataataact ccaaaaggat ttcctgtacc
     4201 taatgcttta acaataatat cttctggaat ccagttgtga atgaaagcac caatgccgac
     4261 acctattaaa acataaggtg caactttttt agctgtctca actacttgtt cccaagcaaa
     4321 cttcattcta tcattaaact gtaattcctc ttgtggcaca tctatcgcat ttcctttgcg
     4381 gataaaatcc tctacttgat tttcaaggtg cattttttca atcagtgtac caccaactac
     4441 tgcaatgaca agacctaata ctacatagac aacagcaact ttccagccga atatactcat
     4501 cagcaataca agagagccta aatcaaccat tggtgacgaa atcaaaaatg aaaaggttac
     4561 accaagtggc aatccagcac ttgtaaatcc tatgaacaaa ggaatggacg agcaagaaca
     4621 gaacggcgtt accgtaccaa gcagagcggc gatgatattt gcccctatac cgtcaaatct
     4681 tccaagtatc tttttcgttc tttcaggtgg aaaataactt tgtatatagg agattatcaa
     4741 aattaaaact cctaataaaa ccatgatttt gatagtgtca tacataaaga actgaatact
     4801 accacctaga cgacttgaag tatccaagcc acaggtattt aataaagctc ctatcagcct
     4861 attcagccac cccattccta ggatttcatt ctgaaaaaaa tcccatccta attctaatgt
     4921 ttccataaat tgaagctcct ttctgatata tatagttata tttaaatata tctatatgtt
     4981 gatgttttat aatgtcgtgt ggaaaagata aacatcactt ttcacagcac gacctttctt
     5041 gatttgtatc ttctgtattt gtgagttcct gtaagcattt caaagcataa accgcaccat
     5101 cattagaaat tgagtaatac gtccattttc catctttacg tccagtcacc acaccagaat
     5161 cgcatagaat tttcatgtga tgtgataagg tgggctgtgt cacattaatt tcttctaata
     5221 atttacaagc acacttttca cctgttacaa gcaacttcaa aattttgatt ctattttcat
     5281 cacaaaacgc cttaaaaata attgcggttt tcttttcatc taagtccatt tattcacctc
     5341 ccatagatat ctgtctatat gtattatatt catcacatag ataaatgtca atatgttttt
     5401 ctgaaatttc ttttgttaaa attttcaatg aagcgtttaa taaatttaac aatttattca
     5461 tcatcaaaag ctgattcatt catttctgga aaatccagca attcagaaac agtcatatcc
     5521 aaacctatcg caagtttgtg aagtgttttc aattttggat tcttcgtctt acctttcatc
     5581 aaattttcta ctgtggattg cgtgatacca gataaagtag caagtctatt gattgtgatt
     5641 tttctttctt cacataattg tgtcaatctt tttataatag cttctgaata tgtcatacga
     5701 aatctccttt actcgtgtac ggttaattca agtatatatc attttcaaac acgatattaa
     5761 cggtacacca ttgacatagt tggcaaaaca gtgtagacta acggtgtacc attaagaagg
     5821 gagagaattt atgtatgaaa ttagcgatat gttgaaaaga agcaaattca gcaatatcct
     5881 gtcctattta acatacggca caggagaaaa tgaagaaatg gtagaaagtt atgactgtgc
     5941 gattgatgaa tcctacgaaa cattctttaa tgatattgaa aacctgtaca cggacgctga
     6001 cagagaagat aacaaacttt tcgatattat aacagagttt gcctgtatac atgatgatat
     6061 ttattttgaa gcaggaatgt tggtgggctt tcaactatac aaagaattga ccgaaaagta
     6121 tgaataccat gcagataagg acatcactgc cattttggaa aagagaaaac agccactcga
     6181 tacagagcat gaagcaaact ccgcattaga atcgttttgt atgcacagac tgaataccgc
     6241 tttagaggaa tctataagaa aagataaaaa ctatcagaaa aaagacgaag aaattgagaa
     6301 agcaattaag cgtatagata aaattggtct gacacataac cagtggaaaa ccgtagacag
     6361 ggctttatcc gcttgtaatg caagaagctc tggatatggg gaaaaagcat acatgcaagg
     6421 tttcaaagat gttataaatc tcatcataga agctatggat caatgagaac acgttcagac
     6481 atgaacatgt tttctcttta tcctcttgat tacccaacac aggaaataaa aagccctgtc
     6541 aagaacctgc caccagaggt ggtgcgaagc attcttgact gggcttttta ctttgctgta
     6601 tcttctgtga gaggataaga gctattctat gcctttcaaa tcttcaaagc cttctatggt
     6661 tggaacgctg gacaaacttt ctctatcccc gtccacatgt ctgttaagta tgattttacc
     6721 ctcaatagga ttccattcat aagggttgat attcctatgc ccctcaatgg tttccccaaa
     6781 caggtcaagg ctgtacaaat catcatactt atcaagtaaa ttctggaatt tatgacgctg
     6841 tatcagttgc ttgatagggt acaggtcaga cgcctgcctg tcctctgaac cgataaagtc
     6901 catgatatat tcacggagtt tctttaactc gtattctttc cattcttcct cgctgtcaaa
     6961 ataattgtca tattgtccat gtgcaagctc tacattcaac cgttcctcac gttccagaaa
     7021 cttcaaaatc tcctgttcca tatgggaagt gttcttccat tccccagaca gcagttcatt
     7081 cggtgtcaca cctaaagcgc aagtaatctt ctccaatgtg tcaaaggtgg gaaaattgac
     7141 acctctttca atcttggaaa ggctctgcat gttgataccg attgagtctg ccagttcctg
     7201 ctggttcatg ccacgaaact tccttaccct ctgtatattc tcccctaaaa gcagatattt
     7261 catgttgtta ttcctcgctt tcttacttat ttaagcctat catacttaat tattttaaaa
     7321 atgtcaagaa atatcattga caaatgagtt taatgggggt attatatgat tagttgattg
     7381 attaagcata acagcgttaa ttatctgctt acataagcat tgaaaagaat tgttttcatt
     7441 ctgaaagcgt taatatcaga ctttcgggat tggaaaacgt ggtttcctaa agtggctggc
     7501 tggacggggc acgtccccca gacgtgttct ggacagacag ttgctttagg aaaggaacgc
     7561 ctttggcgtt ccggcagggc gcataagtaa caccgccctg cgtatacaga actgataata
     7621 gaaaacgggc ttaaaatcag cgtttatcaa ggattttgca acgcacaaaa atggtgcaga
     7681 gaaaggagcg attatttata gcaaaacagg aatttttaac tattaagatt gactacatca
     7741 gcattgtatt cgacactgca acggcagagg aagttatacg gcgtgtcttg gaactgcctg
     7801 ttgatatttt cgacatacag tcggcaaagg tacagcacaa gtcctatcag agcgtctatg
     7861 ccattgggag cattaaagta ttcggcaatg caaagaaaaa tgaggataac cctatggggc
     7921 ttggctctta tcttgtgctt agtgggatgg ggtgtgatga aatcttccgc atactggata
     7981 tgcacggtca gagcttcgga gatttgttca aacggtgtga gcgttggttc tgtggcaagt
     8041 tccatttcac aaggcttgac atagccatag acgacaggaa cgaagtgcct tacttcacaa
     8101 tcaagcagtt aaagcggaaa tgcgaacggg aggaatatat cgctaacagc aacacccacc
     8161 gattcaatga gagcaagtat gaagattaca gcactgcaaa gacggtttat atcggtgctg
     8221 gcaagtctgg catgtcctat cgtttctatg acaaggataa agaggtcagc atgaagcatg
     8281 acaaggctat tgatgatata gggagctgga aacggacaga aatgcagttg cgtgatgaaa
     8341 aggcgcattt gttcgctatg ctgtttaaag acaatccctt tgacttggga aagctggcat
     8401 ttgacctgct ggctggaagt ctgcgctttg tcgttcccga caagacacag agcaacaaga
     8461 gcaggtggaa aacatgccgt ttctggaaac gcttcttagg ggctgttgag cctttgaaac
     8521 ttcatgcaga aacaccgcac aacaccctgt tggaaaccca gcgctggctc aaagagggtg
     8581 gcgtattatc agcagtaaag gcgttccatt tcctagaagt caatcaggca ttaggcaatt
     8641 tggaacgggt ggaagatatg ctgaaacagg tcagatacag tgctaaactc ggcaacaaac
     8701 tgacagggca tttatgcaga atacacaggg aagaactgat accccgtgtg cagtacgata
     8761 caaagaaagg ttaggtggtt cttatagaaa aaggcaacat ggatattcca gtatgggagc
     8821 gttacacgct gaccatagaa gaagcgtcac agtattttcg cataggagaa aataaactgc
     8881 ggaaactggc agaagaaaac aaacatgcag gctggctcat tcggaacggt aaccgcattc
     8941 agataaagcg gaagcagttt gaaaaaatca tagataaatt ggacgctatc taatgaaaat
     9001 gagctttgca tatgctatca tagtgttagt cgtatcaagg ctctttccaa tacggaaagg
     9061 agcatgacag catgtcagaa aagagaagag ataacaaagg gcgcatttta aagactggag
     9121 agagccagag aacagacggc agatacttat acaaatatat agataccttt ggagaaccgc
     9181 aatcggtgta ttcatggaaa ctggtggcta cggacagagt gccaaaaggg aaacgtgact
     9241 gcgtatcgct acgggaaaaa gaagcggaga tacgcaaaga cctagaggac ggaatagacg
     9301 ctgttggaaa gaaaatgacg ctttgccagc tttacgccaa acagaacgcc cagaggggca
     9361 acgtgaagaa aaccacacag aaacagcgtg aacagctcat ggagatactg aaagcggaca
     9421 agctgggagc aagaagcatt gacagcataa aaccgtcaga cgctaaggag tgggctgtca
     9481 gaatgaaaga gaacggctac gcctatcaga ccatcaacaa ttacaagcgt tcgctgaaag
     9541 cgtcattcta caccgccata gaagatgact gtgtgagaaa gaacccgttc aattttgcgc
     9601 tgaatacagt gattgaggac gatagaaagc cacgtgttgc actgacagag gaacaggaag
     9661 aaaagctgtt gtcctttgtg gagagtgaca ggacataccg caagtattat gatgtgatta
     9721 tcctgctttt gaaaacgggg cttcgtattt cagaactgtg cggattgacg gtcagtgatt
     9781 tggactttga gaacaggctc atcaatgtag accaccagct attacggaat aaggaagtag
     9841 gctattacat tgaaacacca aaaaccaaaa gcggagtgcg tcaagttcca atgagtgagg
     9901 aaacctgcca gacacttcaa aaggtggtgg cagagagaaa gaacatacag ccgatagtga
     9961 ttgacggtta ccgtgatttc gtatttctca atcaaaaggg ctatcccatg acaggtgctt
    10021 attatacttc gacatttgga aatcttgtca agaagtacaa caagagccat gaggacgcat
    10081 tgccgaacat cacaccgcat atcctgcgcc acacgttctg cactcgttta gcaaacaaga
    10141 acatgaaccc aaagagcttg cagtatatca tgggtcattc caatatcaac atcacgctga
    10201 acctgtacgc ccatgcgtca ctggatggtg taaaggctga aatggtaagt ctgatagcgt
    10261 aaagaattta ccacttattt accactttcc aaaggcaaaa catgagaatt tatgagaaga
    10321 aacgtgaagt atctaccgat agtaaaaatg cccgtaaagc cgataaaaca aggctttgcg
    10381 aacatataag aatttatgaa aagataatca aatacttatt ttaagtttat attaatctca
    10441 aaaaaaatat aatcagacaa taataatgat aacacataat atagatataa caaaatatgc
    10501 tgatagagtt atagaaatag aagatggatt tataagagag taggtagatt tatgaaactt
    10561 aattatattg taaaaaaata tataatggga aataagaaaa atacattatt aataattgta
    10621 agtattgtaa tttcaacagc gttgttttta attatgaata taataagtga agatgcgaca
    10681 aacttaatga taaatcaagc taaaaatgaa tttacattaa aacatgcaca atatatcaat
    10741 ccaaaagacg aagaaataaa atatatagaa aataacacaa gtatagataa agttggaaaa
    10801 tcaatgcttt taggaattag tgatattggt aaaggtcaaa cactacaaat tctatctcag
    10861 gataaagttg ctgaagaact aaatgatatt tacacactag aaaaaggtaa attaccagta
    10921 gctgaaaatg aaatcgctat agattcatgg tatattaaac aaaaaaagat aaaaaaccct
    10981 ataggaaaaa ggataacatt agattataga agacaaggga tagatcaaga agatttatat
    11041 actggggaaa aagaatttaa aataactggt atcttaaata gtaatcctat tttaaaagct
    11101 caaggaacat caattggcct tataagtaac gattgtgcta tcaagaatat tcctactaaa
    11161 aataaatatg atcaagttat gtttactttt aaaaaggaaa aaaatataca gagacaatca
    11221 caaaaactta ttaaaaatgg aaatttgaat gagaataaca tttattttaa taatgaatta
    11281 ttaatagcta tatctgacag tatgaattta aaaatacctt atataatagt taatatagta
    11341 ctagcactag caactatatt attaatttat aatatttttt atatattagt atcaaataga
    11401 ataaaggatt ttgggatatt tagggcttta gggtttaatc caaatgatat ttttaaaata
    11461 atgattttag aagtatctat atactctatt ataagtattt ctataggtct aattttaggt
    11521 gggataatag ctagtttatc aagggagtat gttataggag taatatacaa tgtcaactat
    11581 ataaattcta taaaaaacca gaactatata aacacataca taatttctat attattaagt
    11641 ttaggaacta taataatatc agtttcaaaa cctcttatgt tatcagttaa aactgatcca
    11701 atgatttgta taagaagaaa taatgaaaaa gtggatataa aacaaaattc ctttataaat
    11761 aaatttatga taaaactttt caaagactat ggtaacatag catcaaaaaa tatacaaaga
    11821 aataagaaac gtacaaattt atctatagca tcaatggtaa ttatattttt cttaatggca
    11881 acaatataca caaagtctac tagtaacttt ttaagtgatg gtgtattaaa atattggata
    11941 caaggagatt atttattaca taatattgat ataagtacta tacgttcaaa taataaaggt
    12001 tatgataaaa atgtactaca agagataaag gcaattgatg gtgtaacaaa agtaaactca
    12061 tataggcata agtggtttaa tataaaaatt gatgataaaa gtataaataa gaactctgat
    12121 tattggaaaa aaaataaaga taatattgaa gctagatcag aagtaaaaga tggaattaag
    12181 gtatataata attcttttga atttttagga atagaagata cagatatact agaggatttc
    12241 ttgataaatg gaaaggaaaa tatagataaa tttagtaaac aaccatatct gtatataaca
    12301 aagaaatcca gtgattcgct taatataaaa aaaggagata gaataaaagt atactttgat
    12361 attatagatt caaaaacaaa taattttacc aaaacaatat ctcaagaatt tatagtatgt
    12421 ggaattataa ctacattacc agtaacctca caagtgggag ctgaatttgg cggagtaata
    12481 tcaacaaatc aatttaataa gtttacagga atatcatctt atgaaagatt tgacatatgg
    12541 acgagtaaac tagccaatga taaatatgtt gaaagtgagt taaacaaaat aacagaaaaa
    12601 acaaataaag gaattttaat tccatacaaa gctgaaacag ctgaatatga aaaaattgat
    12661 aatcaaaaaa cactaattat ggtattagta actggaatta tagttatatt atctttattt
    12721 aactgttgca atactatagt tactagtatt aatagcagaa gtagggaatt tgcactattt
    12781 agaggaatag gaatatcaaa acatgaaata aataaaattg ttaaattgga aagttatata
    12841 tatattatag taagcttttg tatatctata atacctattt taattgtaag aagtgtaata
    12901 ataaaacctt ttgaaagtat caatttaata aattggaaat ttataggggc tattatccta
    12961 atagtattta ttttagtatc tataatcatg ataactacac taaaaacatt gaataaaatt
    13021 caaaatgaaa attttatgga agaaataaag actttatagt gaacagccta taaattaaag
    13081 aggttttata tgaatttaat tgcaattatc gataatggtg tagatattga ttttaaataa
    13141 tttagaaata aaaataaaat aagttatcca agttatatgt atggtgtaat aggggtacaa
    13201 tgtatagcaa atataaatat tggcttaata aaactctact cttaagtcta ctgtcgtttt
    13261 aacacaagac taataataag tgtaacctaa aaattattga taatacaata accttgttta
    13321 tgataaataa caaatattaa gtgttgtaaa gatagtaagt ttaaaaaaat atcaaagatt
    13381 tcaatcacaa tgtattgttt atctgtagtg ttggctattt atggtatata tgtatttata
    13441 aattgtactg aatatatata tactatgcta tctacaaaac agttaacagt cacaggaaat
    13501 gaatatgcca ttataaatta ttatatgagt aactttagtc agtatataat atttgctatt
    13561 ttacttgcat caatgggatt tgtaatacaa aagatagtgc atatgcattt cagtgagata
    13621 gaacttagtg gtaactattc aacaactgtt agaaatataa gtgatatctc tttagataat
    13681 gataatgaaa tgtttaatag aggcaatatt gaaaatagta ttttaaaaaa ataaataata
    13741 taaatatata aaatgtgatt gaataaagct tagaatactt agtgtatttt aagctttttt
    13801 tattattaag atttcaaagt attaaattaa ctatataaaa tcatataaaa ataattttaa
    13861 aatatttaaa tcattgattt caaggtattg tatttttaaa agagtaaaaa tataataagt
    13921 taggtatata atatatataa agtaaaaaat aaaaaaatgg aagtgattga gatgagtagt
    13981 atattgttgg ttgaagatga tttaagtttg atatatggat tagaatattc tatacaaaag
    14041 aatggattta gtgttgatgt agcaagaaca gtagaagaag cattgcaaat ttataaagaa
    14101 aaaaattatg atttactgct tttggatgta tccttacctg atggtgatgg ttttgatatt
    14161 tgtaaaaggg taagagaagc ttctaatgta ccaatcatat ttttaactgc ttctgatgaa
    14221 gaagtaaatg tagtgatggg acttgatatg ggtggagatg attacataac aaaacctttt
    14281 aaattaaatg agttaatttc aagaataaaa gctttactta gacgttataa ttttacaagt
    14341 gaaaatgtaa ctgaactaaa atcaaataat attactgtta aattacttga aaatcgtgtt
    14401 tttaaaaatg aaattgagct tgagcttact acagcagaat ataaattatt atgcttactt
    14461 atgaaaaata aaaatattgt acttacaaga aaaaatatat tggataagtt atgggatgga
    14521 aatggtagtt ttgtagatga taatactctg tcagtatatg taagaaggtt aagaaataag
    14581 attgaagata atcctgaaaa tccaaaaatt ctattaacag ttagaagaat gggttacaaa
    14641 tggaatgtta taaagtgagg gtgttaatat gaaggtattt tctaataaag acataaaaat
    14701 attttttttg gtgattatat ccgttttatt gatgtttata gtagtaggtc aaatagtagt
    14761 tataaatata actaatgatt ataaaagtgc actgttagaa catgattata atatagctgg
    14821 atacttaaat agtatgggtg ttgataaatc aaaaatacca gcagtattta ctactaatga
    14881 agataaatat actttaaaag gaaaagaaat cttaaataca gctagatatg actttggtac
    14941 agatgatagt tttatgccaa gtgtgaaatt gtttcaaggt aaatatataa aaactatgtt
    15001 tttgtattta tttgtgttct ttactatgat tatttttata ttgtatgtgt atttttttag
    15061 gcaacagaac aagatagaag aagctagtta taaaattgat tcatttatga atggaaatat
    15121 aaatgtacgg ttaaatactt atgaagaagg tagtttatca aagttatttg gttcaataga
    15181 tgctatgtca acatctctaa acacacatat attgaaagaa aagcagaata aagaatttct
    15241 taaaaataca atttcagata tatcacatca actaaaaact ccacttacag cattgatgat
    15301 gtataatcaa atattgcagg aagagagtca taatagtgaa attgttgaga attttgtgca
    15361 gaagagtcaa aatgaattag agcgtataga atcattgata caaaacttgt taaagattac
    15421 aaagatagat tcaaagacaa taattcttaa caaagctagt gttaacgtaa aaaagttgat
    15481 tactaaaatt ttatatagtt ttgaaactag agcagaaaaa gaaggaaaga gtatctctat
    15541 aagaggtcta gaaaatacaa acttgttttg cgattatgaa tggatatcag aagctttaag
    15601 caaccttata aaaaatgcat tagataatac aaaagagaac gataaaataa tgatagaatg
    15661 ggttgaaaca cctataacaa ctattatttc agtgagtgat accggaaatg gaattcatat
    15721 tgaagatata catcatatat ttaaaagatt ttataggagt aagttttcaa aaagtaatca
    15781 aggccttgga ttagggttgc cactagttaa atctatcata gaacagcaca atggaaccat
    15841 aactgtagaa agtaattttt tacaaggaag tacatttact ctaagttttt taaagcttac
    15901 taatatgtaa gataggatac atcttacagt aaggtgtatg aattattctt atattaatca
    15961 tcagaaagga gatagagcaa atggatattc ttgtggttaa taatttatgt aaaacatatg
    16021 gaaatggata tacaaaagta gaggcattaa aagatgtatc atttacagtt tcaaagggag
    16081 agtttgttac tgtagttgga gaatctggct caggtaagag tacattatta aatcttttag
    16141 gaggtcttga tactccaacc tcaggaaaaa tatttgttga tggtaaagaa atatgttcaa
    16201 tgaaagaaga aaaacagact atttttcaac gtcgtaatat aggttttatc tttcaatcat
    16261 ataatcttat acccgaatta actgtggaac aaaatataat atttccttta ctactagatt
    16321 acaaaaaacc agacactatt tatctagatg aagtattgac aattttaggt cttaaagata
    16381 gaaaaagtca tcttccaaat caattatctg gtggtcagca acaaagggtt gcaattggaa
    16441 gagcattgat tacaaagcca tcaattatat tggcagatga gccaacagga aatcttgaca
    16501 gtaaaaatag cagtgatgta atttcacttt taaaagtgac agcagaaaaa tataaccaaa
    16561 ctattattat gataactcat aatacaaata tagcttctac agcagatagg atactacagg
    16621 tttctgatgg aatgcttaaa gacctagggg tgaatagaga atgagaagct atttagaact
    16681 agtaccacaa tatgcaaagg tacataagaa aaaaaataga actacaattt tatgtattgt
    16741 cattgcagta tgtttagtta ctgctatttt tggactagca gacatggcta tacaatgtca
    16801 aaaagtacaa tttataaaag atgatggaaa ttggcacata gtacttaaaa atcttaatga
    16861 agaagctata tcagaaataa gtagtagagt agatatagct cactatggta gaggacaagt
    16921 cattgataat ggagagttta aatcaaagga gattttgata caaggtttag atgaagattt
    16981 agcaaaacaa ttaggtctag aaataataaa aggtcgttat cctgttgtgg agaatgagac
    17041 tttaatagat gtagatgcag ttaaaagctt taatctaaaa gtaggagatt ttatcaaaat
    17101 aaaattagca gatggtagaa ataaaaatta taagatagta ggtgaatatt catcattatc
    17161 aagcttaaga gcctcagata agcatggatt aatgctttca atattaggta cacaagctat
    17221 caaaaaatca gaaacatatg attattatta tattacattt aaagacaagg ttaatattag
    17281 aaaagctact tctgatatta agaatacctt caatttaagt gataaacaaa tcggtaaaaa
    17341 tgaaaaatta ctttctatta taggtcaagg tgactctgat tattctaaac cactttactt
    17401 aacagcagga ggacttttta tacttgtgtt aatagcaagt attatcatga tatataatag
    17461 ctttaatatg ggtgtaatag agagaataaa gttttttgga ctattacgtt gcttgggagc
    17521 atcaaaactc caagtcaaaa aatttgttgt tttagaaagt ttaattctaa gtgtaaaggc
    17581 tattccacta ggattgttat taggatgtat tgtaaccatt atatcctcta tatttctcaa
    17641 atatgtgaat acagagttat ttggttcgat gccaatttta aaggttagct ttattggtat
    17701 tgtgtttgga ataatagtag gatttttaac agtaatatta tcagctattg ttccagcaaa
    17761 aaaagcttct ggggtgtctc cactaagtgc tatacgtggt aatctaatgt tcaaagaaat
    17821 tttaactagt ggaaaagcaa gtaaaattgc taacgcagga agttctaaaa ttgatgtttc
    17881 tatgggaata aatcatgcct tttcaagaaa aaaatctttt atacttatga cttcttcatt
    17941 tgctattagt attgtattgt ttttaggttt tagtgtattt atagacttta tgtatcaggc
    18001 acttaaacct ataaaaccag aagctgctca tatatctatt gttgacgctg actttaagtc
    18061 tgttcttact aaagacgata ttaatgaaat aagtaatatc aaaggaataa aaaaagtata
    18121 cagtagaatg atgacggatg tagaggctac atacaataat aaggaggcaa catctcagct
    18181 aatttcttat gaaaaaaatc aatttgactg ggccaaaaag tatctagtaa aaggggaaat
    18241 caatgaagat aaattagagt cagaaaatat cggattggta gaagaaggtc atgggtttaa
    18301 tattggagat aagatatata ttaaaaatgg aaattctaag aaagagatag aggtagcagg
    18361 tatactttct tcaatttcat ttgatgttaa agacaaatat attggaaata ttataacatc
    18421 tgaaaatact tttactagta taacagatat aaaagactat ataataattg atactcaagt
    18481 tgatgaagat gctcctgaag atttaatcca aaatataaga aatgttctag atgataattt
    18541 agaggttcga gataagcgac aaggtaactt agaggctaaa aattcatttt acactatggc
    18601 agtttttgta tatgggtttg tatttataat tgcgattata agtatgttta atatcataaa
    18661 cagtatgaat ataagcgtta caagtagaat aaattactat ggtattatgc gtgcaatagg
    18721 catgtccaat aagcaattaa gaaaaatggt aatagtagaa tcaagtacat atgctgtaag
    18781 tggctgttta ttggggagtg tacttggatt aatattacat agatatatat ttgtatcctt
    18841 agtaacatta aaattccata tagactggca aatacctttt gatttactgt ttatcattgt
    18901 agtaactatg ataataataa ctttgcttgc agtaagaaaa ccaattaaaa agatttgtga
    18961 attagacata atagaggtag taaatgcaca ataaatataa gtagtaattt aaatagaggt
    19021 agtaatttaa ataaaaaatc gaagtaaata tgaaatagaa aatatctttc ataatttact
    19081 tcgatttttg tgttatataa tttactttag ataagttcca tcttcatctt gataaattaa
    19141 gtctttcttc ataagtactc caagagcacg tttaaaattt ttcttacttg aattaaataa
    19201 gatagaaata tctttaggat cagatttatc attgaatctc atatatccat ctgaaccttc
    19261 taaatagctt aaaatcttat tttctaaggt atcaagttca tcttttctgt ttcctctagg
    19321 agaaagtcct aatttaccat catcgtatat ttttataaca tgtaaatttt caagaacatc
    19381 cccagctttt aattctgtaa agtattcatt atgaaggatt acaccagcat atttattgtc
    19441 aacacatatc atagcagagt tgttggtttg gaatccatat acaactccat ttacaacatc
    19501 tcctacattg taagtatggt ctgtttctaa atatgaatct atgtctgtag tagcagctat
    19561 tcttccgctt ttatccaagt atatatagaa taaatatttt tcatctctaa acaatggata
    19621 agtcttagct ttaaaaggaa ctagtatgtc ttttgtaagt cccatatcta taaaagtacc
    19681 aatattcgtg ttatcaacta cttttagata tgcaacatct ccatttctaa ttatttctat
    19741 agtattagaa aaataatttt taaatagttt gtaaaatatt attgaatttt ttctctcgaa
    19801 atatataatt tagaatcttc tgatttattt ttatgctcga tttccttatt ttttttcata
    19861 aatatatata gaggagctat taacaacatt gcccctaatc ctattaaaac tctattttga
    19921 gttataggtt ttgaaatatc ttctgcccaa gcttgttgta acaaccataa gctacctaaa
    19981 atagctacta ttggtataac agggccaaat ggaatcttaa aagaggcctt aacatctgtt
    20041 ctttttctta aaactattac tgataaacaa gttggtatgt actgagcaaa tcttgcaatt
    20101 acgctcatta ctgcaagttg ttcaaaactt ccagttagca caagtggtat acaacaaatt
    20161 aaagatatta ttattgctac atatggagca tcatatttat ttgtttttcc tataaatgca
    20221 ggaagagaac cttcttctgc tagagaagaa ccacatttag gagttactat tgatgaacct
    20281 atatttatac caaatataga tattaatgtt gctacagata tgaaaacctt accataattg
    20341 cctaaaaata cacttgaggc atctgcaatt ggaatactgt tttcaaatag tctatctcca
    20401 agtataccca tacatacaac ttgaatcaat atataaatta ttgcacatat aaatattgta
    20461 gttattaatg caattggaag atttttttta ggattttcca tttctccact tgccacaata
    20521 aatgattcaa agccggtaaa tgcgtaaaat actaatataa tagctggacc catactagag
    20581 ttaactacat ttcctgaagg aacaatattt gcaaatttta taaagaatat tccaactatt
    20641 acaaaaacta ttaatggtac taatttagct atagttatta cattattcat tatctttgtg
    20701 cttttaattc caaataagct atttattgaa agtagtgtta cgagtgttgc agctatataa
    20761 cctttgtatt cagtggcaga ttctggccaa aatgaaccta aagctgttgc aaatcctact
    20821 gctaaagtag accatgatat aattctaata acccaagaca ttgtaccaac ttcaaatcct
    20881 ataaaatctc caaaagcttt tttggagtat agatatgctc ctccattctt atcaaacatg
    20941 cttccaactt ctgcaaaaca taaaagtatt gatagaacta agagtgtagc aaaaatgtat
    21001 ataaacatac tgttttgacc tgctagatta taaactttac ctggtagtaa gaatatacct
    21061 gaacctatta tgctattaat ccctaataat atcatactaa atagacctaa tttcttagct
    21121 ttttccatat taatttatct cctattcttt tattaattac tgtactaatt aaaatacaaa
    21181 gtattcatta catgcatcat aacacctatt ggtaaacaat cttcatctat attaaagcta
    21241 gaggtatgaa gaggtgaaat taagcccttt tcctcatttt tacatcctaa atggaagaat
    21301 gctcctttac aatgttctgt gtaaaaagaa aagtcttcac cacctaaaga cgggtttggt
    21361 ctcaatataa atttttcctc tccaagtaat tctttagtat ttgatataac tgtatctact
    21421 aattcttttt catttataac agcaggatag ttttcatcac ttacatgtaa agtcccaaca
    21481 caaccaaatg cagatgcagt gtctccaaca attttagcta ttttatcaat cataaagttt
    21541 cttgttttgc tatttaaggt tcttaaagtg ccttctagtt ttacatcttc acatattaca
    21601 ttgtgtgcat caccaccata tattttacct atagtcaaaa cagcagcatt atttggttct
    21661 aagtttctac ttattatagt ttgtaatgat gtaacaattt gtgaagctgt aactattgca
    21721 tcaataccat tttcaggata tgcaccatga gctcttttac ctttgatact tatttttata
    21781 gtgtctacac tagcatttaa tgtatcatat tttgtttcga taaggcctgt ttcgatatgt
    21841 ggcataacat gaagtccgaa tatataatcg gccttggggt tttctaaaca cccatcctca
    21901 actaaaaatt ttgctcctcc aaaaccttct tctgctggtt gaaataagaa ttttacattt
    21961 acattaagtt tatcttttat tgaatgtaaa actttacatg ctccaagtaa catggttgta
    22021 tgtgcatcat ggccacaagc gtgcattttt ccagaatata ttgatttgta aggtatatcg
    22081 ttttcttctt caataggaag ggcatccata tctgctctta tacaaatagt tttatctgca
    22141 ttttctttta aaatacatgc cataattcca ttgtgttttg taaattccat ataatcaatt
    22201 ccgatttctt ttaaataaga aattaccttc tgtttagtaa gattttcttc aagagctaat
    22261 tctggaattt tatgtaattc tcttcttatg tttataagcc atggttttat agaattacaa
    22321 ctttctatta caaaatcttt tcctaattct ttggtttgat attttaattt caaagcctgt
    22381 tgcattttta ttcctccaat tgctttttat tgttttatta atataataag caattattat
    22441 gccataattt tcgatggaga attttgtttt ttataagaat attg
//
LOCUS       DAELJO010000048        22456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELJO010000048 DAELJO010000000
VERSION     DAELJO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.048"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..189)
                     /locus_tag="KPB88_001562"
     CDS             complement(<1..189)
                     /locus_tag="KPB88_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0053844.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILIS"
     gene            373..717
                     /locus_tag="KPB88_001563"
     CDS             373..717
                     /locus_tag="KPB88_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG0053845.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            748..2349
                     /locus_tag="KPB88_001564"
     CDS             748..2349
                     /locus_tag="KPB88_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG0053846.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSMRIVVNLSLADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2368..3468
                     /gene="dprA"
                     /locus_tag="KPB88_001565"
     CDS             2368..3468
                     /gene="dprA"
                     /locus_tag="KPB88_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG0053847.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3501..5588
                     /gene="topA"
                     /locus_tag="KPB88_001566"
     CDS             3501..5588
                     /gene="topA"
                     /locus_tag="KPB88_001566"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG0053848.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5753..6538
                     /gene="codY"
                     /locus_tag="KPB88_001567"
     CDS             5753..6538
                     /gene="codY"
                     /locus_tag="KPB88_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG0053849.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6645..7910
                     /locus_tag="KPB88_001568"
     CDS             6645..7910
                     /locus_tag="KPB88_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG0053850.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7941..8711
                     /locus_tag="KPB88_001569"
     CDS             7941..8711
                     /locus_tag="KPB88_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0053851.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8820..9266
                     /locus_tag="KPB88_001570"
     CDS             8820..9266
                     /locus_tag="KPB88_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0053852.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9284..10477
                     /gene="nifS"
                     /locus_tag="KPB88_001571"
     CDS             9284..10477
                     /gene="nifS"
                     /locus_tag="KPB88_001571"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG0053853.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10531..10971
                     /gene="nifU"
                     /locus_tag="KPB88_001572"
     CDS             10531..10971
                     /gene="nifU"
                     /locus_tag="KPB88_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG0053854.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11260..12339
                     /gene="mnmA"
                     /locus_tag="KPB88_001573"
     CDS             11260..12339
                     /gene="mnmA"
                     /locus_tag="KPB88_001573"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG0053855.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12482..12720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12788..15427
                     /gene="alaS"
                     /locus_tag="KPB88_001574"
     CDS             12788..15427
                     /gene="alaS"
                     /locus_tag="KPB88_001574"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG0053856.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15452..15709
                     /locus_tag="KPB88_001575"
     CDS             15452..15709
                     /locus_tag="KPB88_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG0053857.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15719..17890
                     /locus_tag="KPB88_001576"
     CDS             15719..17890
                     /locus_tag="KPB88_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053858.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18096..18521
                     /gene="ruvX"
                     /locus_tag="KPB88_001577"
     CDS             18096..18521
                     /gene="ruvX"
                     /locus_tag="KPB88_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG0053859.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18599..18886
                     /locus_tag="KPB88_001578"
     CDS             18599..18886
                     /locus_tag="KPB88_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG0053860.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19168..19626
                     /locus_tag="KPB88_001579"
     CDS             19168..19626
                     /locus_tag="KPB88_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0053861.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19743..20480)
                     /locus_tag="KPB88_001580"
     CDS             complement(19743..20480)
                     /locus_tag="KPB88_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0053862.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20697..22373
                     /locus_tag="KPB88_001581"
     CDS             20697..22373
                     /locus_tag="KPB88_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG0053863.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
BASE COUNT         9200 a         2298 c         4167 g         6791 t
ORIGIN      
        1 agaaataagt ataaatttat tctcatttga taaagaaaaa aatctaactt gattatttat
       61 tataattaga aataatatta tcatagttat gaccggatac aatgtatttt caacattttt
      121 caaatagctt acaactaata taaataaaga taaatatcgt ataaaagcaa ctaatttttc
      181 tctgttcatt aaaggcactc ctccaaaaaa ataaactttt cctatgaaaa ctagaataat
      241 atcataaaag aataaagtca atagaagtat ttattaatat tgatggaaat aaaatcaatc
      301 caaaaacttg aatagtaaaa ttgtatactg accataattt aaaagtgaaa atttaatgta
      361 ttggagtgaa taatgaataa taaagaaaaa ggtgattttg gagaaaaagt agcagttaat
      421 tatttgctat caaaaggagc aaagatacta gaaaaaaatt acagattaaa gataggggaa
      481 atagatataa tagcaaaaat ggaagatgaa attatatttg tggaggtgaa atcaaggtct
      541 aacataaagt ttggctaccc ttgtgaatct gtaagcttca aaaaaagaaa aaagataatt
      601 ggagttgcaa gttattatat tataaaaaat aacttaaata acactcccat aagatttgat
      661 gttatagagg tatatttatt agaaaaaaga ataaatcaca taatgaatgc tttttaaaaa
      721 agataatgtt tttggaaagg attaaatatg ttaagtatta ttaattcaag caacttagta
      781 ggaatcgaca gctttttagt taaggttgaa gtagatgtaa gcaatggaat acctagtttt
      841 aatattgttg gtcttccagg caaagaaatt aaagaagcaa gagaaagagt aaagtcagca
      901 attcttaata gtggatataa atttccaagt atgagaatag ttgtaaattt atcactagca
      961 gatataaaaa aagaaggagc ttttttagat ttatctataa gtataggcct tttaagagag
     1021 ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt ttataggaga attatcatta
     1081 gatgggaaaa taagaaaagt aagaggtata ttgcccataa ttatgggtgc aaaaactcag
     1141 aacataaaac gtatttttat accaattgag aacataaaag aaagtctatt tgtagatgaa
     1201 attgatataa ttcctattaa aagtttaaaa gaatgtgtcg attttttaaa cgaagagata
     1261 aaagtcgata aagtaaggat aatgtctttt ttggatgata agtcaagaaa agagaacgaa
     1321 gaattagaaa aagataatag ttatatagat tgcaaatata ctaagattaa taatgaagaa
     1381 agtaaatatg atgaagactt taaagatgta aaaggaaatt attttgtaaa gaggagtgca
     1441 gaaattgctg ctgctggaaa tcataatatg tttatgattg gacctccagg ctcaggaaaa
     1501 actatgattg caaaaagagt aaggacaatt ttaccagata taagtgtaga agaaatgata
     1561 gaagtaagta aggtctatag tatattgggt atgataaatg aaactaaagg aataattgat
     1621 aaaagacctt ttagagcacc tcatcataca actacaaagc aatctttaat tggaggagga
     1681 atggatgcaa gacctggaga aattgcttta gcacatagag gtatcttatt tttagatgag
     1741 atagccgaat ttgatagaaa aatacttgag actttaagac aacctataga agatggatat
     1801 gtaaatatat ccagagtaaa gtacagtgca aaatatcctt gtagagtact gttagttgca
     1861 gctatgaatc catgtccatg tggttattat atgtcagaga cagagtgtag atgtagaagt
     1921 aacgaaattg atagatacat aaataaaata tcaggtccat tgttagatag gtttgatatt
     1981 tttgtagaag ttaattctat aaaatattca gattttaatt ctttaaaaca agaagaaagc
     2041 tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa tacagataaa tagatttaag
     2101 aaggataata taaaaaataa tagtgaaatt aaagcttaca atttatttaa atattgtaaa
     2161 ctggaaaaag aagcttcaaa aacggctgag atgatattta ataaatacaa cctaagcagt
     2221 agaagttata caaaactttt aaaaatggca agaacaatag cagatttaga agaaagagac
     2281 ttgataaatt cacagtgtat tatagaagct ttttcattta gaaaggctta ttatagttat
     2341 tttaaatagt tttttgaggt ggattttatg gaaaaaagag atacttattt atggttaaaa
     2401 tcaattggtg gaataacaac aaaaacaatt gaaataatag aaaatgaaat agtaaatatt
     2461 gaagatatat ttgatttttc tgaaaaagaa atatataatt taaaaaatat aagcttgaat
     2521 attagaaaaa atatagtaaa atatagaggt cacgcttatt tagaaaatat aaaagaacta
     2581 ctttacaaaa aagcgattaa atatatttgt aaatatgata aagaatatcc tgaaaattta
     2641 aagaatatat acaacgctcc aaagttatta ttctacaaag gtgacatagg tttagttaat
     2701 aataatttta atatagctat agtaggttca agaaaaccta cagcctatgg aattaattgt
     2761 gcaaaaacaa taagttgtca actatcccaa tatggagtta atatagtaag tggattagct
     2821 ataggaatag atgcatactc gcacataggt tgtatgagtg gaaaatctaa aactatagca
     2881 gtacttggtt caggagtaga caatcctctt ccaaaacaaa atttacattt atcaaataaa
     2941 atattagaaa atgggggatt attattatca gagtataata ttaactcgac agtagccccg
     3001 taccattttt ctaatagaaa tagaattatc agtggtttaa gtgatggagt agttgttgta
     3061 gaagcagcaa taaaaagtgg tgcactaatc acagtagatt ttgctcttga acatggaaaa
     3121 aatgtttttg caatacctgg taatataaat tctcaaatga gtagaggttg tcataaaatt
     3181 ataaaagaag gtgcaaaatt aattgaaaat atagatgata ttttaaatga atataacata
     3241 tttaatatta ttgataaaaa aataaatcaa aaatatgata atataagtct aaatgccaag
     3301 tctaagcaaa taattgaagc tataaaaaga gaaggaaact tgcatataga tagtatttgt
     3361 gattatactg gtatagaaat aaagtatgta aattcaataa tcaatgaatt agtactaaat
     3421 gagttagtag tagaaatgaa taataaaacg tacagtttaa atgtataaat taagattagg
     3481 tagtgggggt gtatggtttt atggccaaaa cattagtcat cgtagaatcg cctgcaaaag
     3541 ctaaaactat agaaaaattt ttagggaaaa gtcattacac agttaaagca tcagttggtc
     3601 atgtcagaga tttacctaaa agtaaattag gtgtagatat agaaaataat tttgaacctc
     3661 aatatataaa tataagagga aaaggcgatg taataaaaga attaaaaaaa gaagctaaaa
     3721 aatctaaaca agtatatcta gcaactgacc ctgatagaga aggtgaagct atatcatggc
     3781 atttagctca tatactaaat ttagatgaat ctgaggattg tagaatagag tttaatgaga
     3841 taactaagga tgcaattaaa aaagcaatta aacatcctag aaatattgat ataaatttgg
     3901 ttgatgcaca acaggcaaga agagtgctag acagactttt aggttatcaa ataagtccta
     3961 tattatggca aaaggttaga aaaggtctta gtgcaggtag agtgcaatct gttacaacaa
     4021 agttaatttg tgacagagaa aaagaaataa aggcatttat tccaaaagaa tactggacaa
     4081 tagatgttga ggctaagaca gtaaaagaca aagatataac tttgaaattt tatggtaaaa
     4141 atgatgaaaa gattgaatta gaaaatgaag aagctgtaaa tgaaatacta aaagaaatgg
     4201 aaggtaaaga tttaaaggtt gagaagatag aatctaaatc aagacgtaaa tcagctccaa
     4261 aaccatttac aacaagtatg cttcaacaag aaggagcaaa taagttattt tttactacta
     4321 aaaaaacaat gatgatagca caagaattat atgaaggtat cgacatagaa ggtgaaggta
     4381 ctgttggtct tatatcatat ataagaactg actctaaaag aatatctgat gaagcaaaag
     4441 aaaaagctaa gggctatatc gtaaacaatt taggagaaaa ctactataaa aacactggtg
     4501 ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc ccatgaggca ataagaccaa
     4561 cctcagttga tagaactcct gagagtataa aagcctcatt gagcaaagac cagtacaagt
     4621 tatacaattt aatttggaga agatttgtag ctagtcaaat ggaagattca gtgtttgata
     4681 tactaaatgt agaatgtaaa ataggcgata ttgtatttaa ggcaactggt tcaaaaatga
     4741 aatttgatgg ttataccaag gtatataatt tttcagaaag agaagacaaa attttaccaa
     4801 gtatagaaaa aggagatata ttaaatatag aaaatatatt gccagaccaa cattttacac
     4861 aaccaccagc aagatataca gaagctagtt tagtaaaaac actagaagaa ctaggtatag
     4921 gtagaccaag tacgtatgct ccaacaatag ctactatact gaatagagag tatgttgaaa
     4981 agcaaggaac aagtctatgt ccaacagaac ttggaataat agttactgat attttggaaa
     5041 ataattttca taaatttata gatgtagatt ttactgctga tatggaaagt aaacttgatg
     5101 ttatagaaga aggcaataca gattggaaaa agattgtatc agaatcatat gcaccactta
     5161 aagaggctat tgaagaagct cttgaaaata tagaaaaagt aaatatggat gaagaaactg
     5221 atgagatttg cgagaactgt ggttctaata tggttatcaa atatggtaga tttggaaagt
     5281 ttatggcttg taaaaactat cccgattgta aaacaactag accaattatg aataaaattg
     5341 gtgttaaatg tccaaaatgt gaagaaggag atattatact tagaaaatct aaaagaggta
     5401 aagcttttta tgggtgttct aattatccaa actgtgactt tgtagcttgg aataagccaa
     5461 caggagagtt ttgtgagaag tgtggttcat atatggttga aaaagtgact aaatctgaaa
     5521 caaaaacagt gtgttctaat aaagaatgta ataaagagac aaaagagcat aatgacggaa
     5581 aagagtgaaa attgaaaaaa atcacataat tctcttgaaa aaggttccaa tctatgataa
     5641 tctttcaagt gtaaacaact aatagaaaaa taaattttca ttaaatgtga aaataatatt
     5701 agaaaatata aaaggttatt aaaaggccta aatttcatga ggagatgatt aaatggcaag
     5761 tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta caaacaagtg gtggaagcag
     5821 tgtctctttt gatttactgg ccggagcatt gggcgacgtt ttaagttcta atgtttatgt
     5881 agtaagtgca aaaggtaaag tactaggtct tcatttaaat gatgttcaag acagttcagt
     5941 tatagaagat gagtatacta agcaaaagaa attttcagat gaatatactc aaaatgtgtt
     6001 aaaaattgat gaaacattag aaaatttaaa tggtgagaag atattagaaa tctttcctga
     6061 agaacatgga agattacaaa aatatactac agtagttcca atattaggaa gcggtcaaag
     6121 attaggaaca ttggtacttt caagatattc aaattcattc aatgatgatg atttagtaat
     6181 agctgaatac agtgcaactg ttgttggtct tgaaatatta agagcaatag gtgaagaatt
     6241 agaagaagaa atgagaaaga aagctgtagt tcaaatggca ataggcactc tgtcctactc
     6301 cgagcttgaa gcagttgaac atatttttgc tgaattggat ggaaaagaag gtctacttgt
     6361 agcaagtaag atagctgata gagttggtat aactaggtct gtaatagtaa atgcacttag
     6421 aaaatttgag agtgcaggtg tgatagaatc aagatcatta ggtatgaaag gtactcatat
     6481 aagaatactt aatgacaaac ttacagatga attaaaaaaa ttaaaaaaca atcaataatc
     6541 tatattttat aggtttagat tagaaaaggt atctatttca aatagatacc ttttttagac
     6601 gaattattaa ggtaatgata agtatattaa aaaggagtga gaaaatgcct ttagcagata
     6661 aaataagacc aaaaacaatg aatgatgtaa taggtcaaag tcatataata ggtaatggaa
     6721 aaatattgtc taagatatta caatctaatt ttttaccaaa tatgatattt tttggtcctc
     6781 ctggtgttgg taaaactaca gtagcggaaa taatagcaga aagaagcaat aaaagcttct
     6841 ataaaataaa tgctactaat tcatctttag aagatataaa aaaggtgata tcagagcttg
     6901 gaagtataaa taatgtaaat ggagtcctat tatatataga tgaaatacaa agttttaata
     6961 aaaaacagca acaatcaata ttagaattta tggaaaatgg gagtataact ttgattgcta
     7021 gcactacaga aaatccatat cattatgtat ataaagctct tttaagtagg tctactgtat
     7081 ttgaatttaa acctttagaa aaacttgata tagaaaaagg actgaaaaga gctgttgaag
     7141 tattaaatga agatagctat atggatattg agtgcaataa taatgctatt gaagatatag
     7201 caattctttc tgatggagat atgagaagag ccttaaatat tttggaagtg gtagtctatt
     7261 caactaaacc aaataaggat aatataactt atatagattc agatgtaata agggcatcta
     7321 catttaataa aattataaac tatgacaaaa atggtgatag tcattatgat attttaagtg
     7381 catttcaaaa atctataaga ggaagtgacc cacaggcatc tatacactat ctagcaagac
     7441 ttataaaagg tggggatttg ataagtatat gtaggagact tttagtaata gctagtgaag
     7501 atataggttt agcttatcca aatgctattg taattgtaaa ggcatgtgta gactcagcta
     7561 tgcaacttgg atttccagaa gcgaaaatac cattagcaga agctactatt cttttggcta
     7621 catctccaaa gtctaattct gcatgtatag caataatgaa agctatggat gaactggata
     7681 aagagtttgt taaggatata ccaaatagta taaaggatgc tcactattct ggttctaaag
     7741 aaataggtag gggttgtgga tacaaatatc ctcataattt taaaaatcat tatgtaaagc
     7801 aacagtattt accagatagc ataaaagata gtatttatta cataccacaa gaaaataaaa
     7861 ctgagaaaaa tataaaaaaa tatttagaat atctaaatag tgatttttag actgaacttt
     7921 atgtatcggg ggtagatatt atgattataa aacttaactc aacttatcac agtagagtta
     7981 tgaaatactt aaaaaaagaa cctgagtaca atttgtttat aataggggat attgaaagat
     8041 atggatatgg aaataacttt ttaaatatat gggcagatgt gggtgaacat ggtgaaataa
     8101 aggcaatatt acttaaatat tttgaattta tgatgtttta ttctgatgga gagtatgatg
     8161 ttgaaggttt ttataatcta cttcgtaata ctaattacga agaaatatct gggaaaatat
     8221 gtgctgtaga tgctttagca aagagattag gacttaataa tttaaaggta gttgattttt
     8281 gcaaacttca aactaaaaag tttttgatag ataataattg taatgcaaag gttaagagaa
     8341 ttagacttgg aaatctaaaa aagacagtaa aactttatga cttaatagat gaatttcaca
     8401 gtacaacttt agagaattta aaaaatggac taaggacagg tagaggatat tgtatagaga
     8461 ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga aaataggact catgcaatga
     8521 taattggagt aggaacacat cctaaatata gagctaaagg acttgctaca aaatgcctaa
     8581 ttaaactatg ttcagagtta ctgagagaaa acaaaattcc ttgtttgttt tatgataatg
     8641 aagaggctgg aaagatatat aaaaaactag gatttgaaaa cattggaaaa tggggaattt
     8701 attcaaaata aaaaattaaa attaatcttg acaaaattag tagggtataa taaaattatc
     8761 ttgaaagtaa ttcctactaa aacagtatga attagatttc tatatagagg tgataatata
     8821 tgaagttgtc tactaaaggt agatatggac taaaagcaat gtttgaatta gcattaaatc
     8881 aagacaatgg accagtatca ttaaaattta ttgctaagaa gcaaaaaata tcagatcagt
     8941 acttagaaca aatattttca tctttaaaaa aatcgggtct agtaaaaagt gtgagaggtg
     9001 ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat aacagtagga gatatacttg
     9061 tagtattaga aggaccagtt gcattatctg actgtgtact agatgaagat gtatgtgaaa
     9121 actcaaacat gtgtgtaaca aaaatagttt gggaaaagat gaaaaagggt atagaagatg
     9181 ttatagattc aattactctt aaagatatga taaatgacta taacaaaaat aaattagaaa
     9241 atgatataac aaatattaaa aaatagattt ggagatgaat gtaatggaaa aaagaagatt
     9301 gtatatggat tattctgcaa caacacctat taaaaaagaa gtattggatg caatgatgcc
     9361 atacctaaca gattattttg gtaatgcatc tagttttcac acattcggaa gagaagcaaa
     9421 agatgctttg gataaagcaa gagaacaagt agcagccctt ataaatgctg aacctagtga
     9481 aatatatttt acagcaggtg gttcagaaag tgataactgg acactagagg gtgtagctta
     9541 tgctaataaa aacaaaggta atcatataat aacttcaaaa atagaacacc atgcaatact
     9601 tcatacatgt gaatacttag caaaacatca tggatttgaa ataacttatt tagatgttga
     9661 tagcgaagga aaagtagact taaaacaact agaagagtca ataaaagata ctactatact
     9721 tataagtata atgtttgcaa ataatgaaat aggaactata cagcctatta aagaaatatc
     9781 cgaaatagct aaaaaacata agatattatt ccacactgat gcagtacaag ctacaggtaa
     9841 tattccagta gatgttaaag aattaggaat agatttgatg agtatgtctt cacataaaat
     9901 atatggacca aaaggtgtag gagctttata cataagaaaa ggtgtaagac ttcataactt
     9961 tgtccatggt ggagcacaag aaaaaagcaa aagagctggt acagaaaata tacctgcaat
    10021 agttggatat ggaaaagcag cagaattagc taaggaaaat atgcaaaatc atgtagaaac
    10081 tttaactcgt cttagaaata agttaataga tggtgtgtta gaaagaatac cttacacaag
    10141 agtaaatggt agcttagaaa atagattgcc aggaaatgca aactttgcat tccaatttat
    10201 agaaggtgaa ggaatacttt tattattaga tatgttagga attgctggtt caagtggttc
    10261 tgcatgtact tctggttcat tagacccttc acatgtactt cttgcaatag gtttaccaca
    10321 tgaaatagca catggttcat taagacttac agttggagat tttacaacag atgatgatat
    10381 agattatata ctagagaact taccaaaagt aatagaaaga cttagaagca tgtcaccact
    10441 ttatgatgat gcgaagaaac aaggtttagt aaaatagaca taaagataaa gcataaaaaa
    10501 tagacataaa ttgagtagga ggaaaagata atgcaatata gtgataaagt tatggaacat
    10561 tttatgaatc caagaaatat gggagaaatt gataatgcaa gtggtgtagg tgaagttgga
    10621 aaccctacat gtggagatat aatgaagata tttttagaca tagatggaga tgtaataaag
    10681 gatgttaagt ttaaaacatt tggctgtggt tcagctatag caagttcttc tatggcaact
    10741 gaaatgataa aaggaaaaac tataaaagat gcattagaac ttacaaataa agctgttgca
    10801 gaagctttgg atggtttacc accagtaaag atgcattgtt cagttttagc tgaacaagct
    10861 gtaaaagcag ctcttataga ttatgctcaa aagaacaata tccatatacc agaattggat
    10921 ggatatgtta tagatgatgc tcatgaccat gatgttgaag aagaagaata aaaataagtg
    10981 attgtgttat gagaagttta caatgattaa aaagaagtac taatgatata attaggacat
    11041 aaaaaatagc tgtttttaaa atggctattt tttattgcct aaattaagta ttttaacttg
    11101 atttagaggt taattagtta acaaaataat ccataagatt caaataataa agatgattat
    11161 ttttttagaa aatagaacaa gctatatttt aaataagtac aatacttgct ctatatggta
    11221 taataataag taatgcaaaa gatgaggtga aatttgttta tgaataaaaa agtaatgata
    11281 ggtatgagtg gaggagttga tagctctgta gctgcatatc ttttaaaaca acaaggatat
    11341 gatgtcattg gtgttactat gaagttgtgg caagatgatg atgttgttga aattgaaggt
    11401 ggttgttgct ctttatctgc tgttgaagat gctagaaggg tagcaaataa aataggtata
    11461 ccattttatg ttttaaattt tagagaagtg tttaaagaaa aagtaataga ttattttata
    11521 gatgaatatt tggaaggaaa aactcctaat ccctgtatag cttgtaataa acatataaaa
    11581 tttgatgatt tttataagaa ggctagacaa ataggttgtg attatgtagc tacaggtcac
    11641 tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa atctgttaca
    11701 gataaaaaag accaaactta tgcactttat aatcttacac aagagcaact agagcatact
    11761 ttattgccaa taggtgacta tgaaaaagat agagtaagag aaattgctaa ggaaatgggt
    11821 atggcagtac acaataagcc tgatagtcaa gaaatttgtt ttgttaaaga taatgactat
    11881 gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt tgtagatact
    11941 aaaggcaata tacttggaaa acataagggt atattatatt atactatagg ccaaagaaaa
    12001 gggcttggaa ttacttttgg aaaaccaatg tttgtaatag acataaatcc aataaataat
    12061 accatagttc ttggagacaa tgaagattta tttaaaaaag aacttattgc taaagatgtt
    12121 aactttatat caatagatac gttagaagaa cccttaagag ttcaagccaa aataagatat
    12181 tcagcaaaac catcaccagc tactattcat agagtaggag aagatactat taaaatagtt
    12241 tttgatgaag ctcaaagagc tattactaaa ggtcaatctg tagtaatgta tgatggagat
    12301 attgtagtag gtggaggtat aatcgagaaa agtttataaa aacatctaaa aaattaaaaa
    12361 aataaacaat aaagtatatt aagagtaagt aaagtttata aaataaattt attgtatatg
    12421 aaaatatagt attgattaat tttgtaaaat ttagtaatat aattagtata ttaattaaaa
    12481 aacaaataca gtgataagga atagtaaaat atttgatgtc ttacagagag agggaaatgc
    12541 tggaagccct tagaatatga tatttgaagc agccttttag tagcaatctt gaacaattta
    12601 tcttttataa agaagattta agtagagatt gacggtagtt acgttatagc taaggttttc
    12661 cgaagatatc atactttgta tgataattag ggtggtaccg cgattgtcct cgcccctata
    12721 aatttattta tagggacgag gttttttatt taaacgaaaa aataaaattt attaggagga
    12781 acatgatatg gaaaagatgg gattaaatga aataagaagt aagtttttag aattttttga
    12841 gtcaaaaggt cattatgtag caaatagtta ttctttagta ccaaacaatg ataagagttt
    12901 gttacttata aactcaggta tggcaccttt gaaaaactac ttttcaggtg tagaagtacc
    12961 tccaagtgtg agaatgtgta catctcaaaa atgtataaga acaggagata tagaaaatgt
    13021 aggtataact gcaagacatg ctacattctt tgaaatgatg ggtaacttct catttggaga
    13081 ttactttaaa agagagtcaa tcaaatgggg atgggaattt gttacagaat ggctaaatat
    13141 accagaagat aagatatggg tgactgtata tgaagaagat gatgattctt atgatatatg
    13201 ggcaaaagaa atgaatttcc cagaagaaag aatggttaga cttggaaaag atgataattt
    13261 ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa atatattttg atagaggaga
    13321 agaatatgga tgtgacaatc cagactgtaa accaggctgt gattgtgata ggtatttaga
    13381 attttggaac catgtattta ctcaatttga tagagatgaa gaaggaaatt atagtctatt
    13441 agaaaacaag aatatagata caggtatggg tcttgaaaga atgggatgta ttatgcaagg
    13501 cgtagacact atctttgaag ttgatacaat taaatctata ctagaagcag ttgagaagtt
    13561 aacaggagta aaatatggag agaatccaaa aaatgatata tcaataagaa taataactga
    13621 ccacataaga gctgtaacat tcttagttag tgatggagtg cttccatcaa atgaaggaag
    13681 aggatatgtt cttagaagac tccttagacg tgctgctcgt catggtaagt tattaggagt
    13741 aaaagaatta tttttacaga aactaataga tgaagttata aaagtaaatg ataaagcata
    13801 tccagtttta gttgaaaaag aaagttatat taaaaaagta gttggaatag aagaagaaaa
    13861 attcaatgaa actatagacc aaggaacaga aatattgaac tcttatatag aagtattaaa
    13921 aaatgaagga aagactgtcc taagtggtca agaagctttt aaactatatg atacttatgg
    13981 cttccctata gacttaacta aagaaatatt agaagaagaa catctatctg tagatgaaga
    14041 agcttttaat gaagaaatgg aaaagcaaaa agaaagagct agaaatgcta gaggaaatat
    14101 ggatggagaa agttggaagg aagaccctct atcaaaatta gaatctacag ttgacagtac
    14161 atttaatggg tatagtgaaa tttatggtga aggaactatt gaagctatag ttaaagatga
    14221 tgagctagtt caaagtgcag aagaaggcga taaggtatct atagtactag ataacactac
    14281 tttctatcct gaaggtggag gacaagttgg agattgtgga ttgataacta atgaaaactt
    14341 agttttagaa gtattaaata ctaaaaaagg tgctaataat agtataaaac atataggtat
    14401 aataaaatca ggaagaataa gtaatgggga caaagtaaag acactcgtag atagagaaac
    14461 tagaatgtca gctgcaagaa atcacagtgc tacacattta cttcataagg cattaagaga
    14521 agtattagga gaacatgtta atcaagctgg ttctttagta actccagaaa gacttagatt
    14581 tgatattact cactttgaag cgatatcaaa tgaagaatta aaagtaatag aagaaaaagt
    14641 aaataatgtt attttatcat ctttagacat aaaatgtgat attatgaata taaaggaagc
    14701 taaagaaaaa ggagctacag cactatttgg tgaaaaatat ggtgatgagg taagagtagt
    14761 ttctatggga gattattcta cagaactttg tggaggaact cacttgacaa atacttctca
    14821 agtaggaatg ttcaaaatat tatctgaagg tggagtagca gcaggtgtaa gaagaataga
    14881 agcaataaca ggaaaagctg tttatgaata cctaaaagaa agagatggaa ttatctctga
    14941 agtttgtgta aacttaaaat ctaaagaaga taatttaatt caaagaataa gttctttatt
    15001 agaagaaaat aaaaacttat ctaaagaatt gcatgatatg aaagctaaga tgagtttaca
    15061 atcagttgat tctatttttg attctaaagt tgaagtaaat ggagttaatt tgataactaa
    15121 taaatttgaa ggtatggata tggatacact aagagaaaca gcagataatc taagagataa
    15181 gcttggttct ggtgtagtag tacttgcaaa tgtagtagat gataaagtta actttgtagt
    15241 tactgccact aaagatgtat tagataaggg aatacattca ggtaatattg ttagagaagt
    15301 tgcaaaaata gctggaggaa aaggtggagg aagacctaat atggcacaag caggagctag
    15361 tgatgtatct aaggttgacc aagctttaag ctatgcaagt gaggttatta agacacaagt
    15421 taaataacat tcactcaagg aggtttttag tttggaaaaa gatttagact acactatgaa
    15481 atttgaagga ataccagaag ataggatgag tgtaggagat actattgatt ttgtatataa
    15541 agcattagtt gaaaaaggat ataaccctat aaatcaaatt atagggtatc tcctttctgg
    15601 tgattctagc tatataacta gtcataaaaa tgcaagagct ataattaaaa aatttgaaag
    15661 agatgagata cttgaagaag taatcacgca ctatctgaat agaaagtagg tcaaataaat
    15721 gagaaaaaag attatgtcgt tgtttgcaat agtcattatg atttttgtat caactgctag
    15781 ctatggtttt gctgaagaaa aaggcaaggt catatttatt gatatgaata ggaccaatct
    15841 tggaagtatg atgaatattc caatcttgaa aaatgagata gaaaagagag gctatgtagc
    15901 tctcatgaat ataagagggg atcaaggtac tgatgataga aggtcttatg caagtatggg
    15961 tgcaggaaca agagctagtg taactactga agactatatc aattttcaaa aagtagacca
    16021 agatactgct aaagcttttg aagtcgcgac aggtcaaaaa gctaaagaaa taaatgattt
    16081 aggcattaat agatctataa atgaaaatct tgagtatgga gaatatggag caaatttagg
    16141 gtcattagga aaaactttat cagaaaatgg tttaaagaca tctgtaattg gaaatgctga
    16201 tataattgaa aataatcaat tagttaaaaa cagaaactta tgtttagtag ctatggacca
    16261 atatggaagg attgattcag gtaatattga taatattaat aagaaagatt ctaaaatgcc
    16321 atttggaata tctacagatt atgataaatt actagatgaa actaaaaaat cctataaaaa
    16381 tagtgatgct atatttgtag aacttggaga tacatataga ttagatatgt atagattaaa
    16441 tctaaatgat aatacttatg atacaatgaa gaaaaatatt gtaaaaaata taaataaata
    16501 tcttgaacaa gtattttcta tggttggaga aaatgacact gtttatatag caagtgcatt
    16561 tccaagtgac ctagattata agaataagag aagactttct ccaataataa aatttagtgg
    16621 agaagggaaa ggtctactgg aatctgccac aacaagaaag gctggaattg ttgccaattt
    16681 agatgttgga gtagatatac taggtacatt tggtcttgaa aataaagaga tggtaggtag
    16741 aagttatact ttagtaaata aagaggacaa tattagctat ctaaaatcac tttatgaaag
    16801 gatagtgtct atatcttcaa taaggtcaac tattataaat ggctttgtag gcgtaatatc
    16861 tgcttcttgg gtaattgcca tgtttgcgat tttctttaga aattatatgc cacataaaga
    16921 gcgtgtattc aaagtactta aggagtttat aaaattaggc ataataatgc cactatcatt
    16981 tatgttagct cctttgatga attttaaaac tccaatttca atatctttgg gaatatttgc
    17041 gactacgcta atattatact tgttaggtag aaaattattt aaggacaatg atataaagca
    17101 catgggattt tttgcaacac ttactatttt agtcatagtt atagattctg tatttggaac
    17161 atacttaatg caaaataaca taatgagtta tgatgctgtc ataggagcta ggtactatgg
    17221 tgtaggaaat gaatatgaag gtgtaactat agcaagtgca gtatttggct tagcagtatt
    17281 attacattat aagaaggtat ctaaattact tgttgtaatt ttttcattaa taatacttat
    17341 aactagtgca tatccttcaa tgggtgcaaa tgttggtgga gctatatcag aatgtgtagc
    17401 atatttgttg tttataatgc ttatatatga cattaaaata gattttaaga aagctgtatt
    17461 attagctgtt tcagctgtag ttgtcgtttt tgcatttgca gctttagata tactttcagg
    17521 tagtgaatct catttaggta tgtttgtaca gagaattttc ctaaatggac caggagagat
    17581 attacaaaca tttggaagaa aaattcaaat gaatatgcaa ctagcacaga caagtgtatg
    17641 ggtaaatatt ttacttgtag gaattggagt aattgcagtg cttatattta aaccatctag
    17701 acactttaga aagataatga ataattttcc aatcttgttt aaagggttta ttgcatctat
    17761 ggttggttgt ataattactc ttttagttaa tgattctgga atagttgctg catctacagc
    17821 atctatatat atacttatac cactaataat aataagtata aatatgataa tatttgaaaa
    17881 aaataactaa aaaaattttt actgttcata taggatttaa ataaaaaatg caatatgatt
    17941 attaatgtaa actggatgct tacataaaaa atcatattgc aatttttatg attgattatg
    18001 taaaattata tgaagcagta agcctgtgac cttagttatg agaggttcag aatatgtatt
    18061 taatctatag aatataaata gataaaggag gaactatgtt agatggaaga ataatgggtc
    18121 ttgatgttgg tgataaaact attggagttg ctgtgagtga tttgatggga ttaactgcac
    18181 aaggtgtaaa aacaataaaa agagttggca agaaaaaaga tattgaagag ctaaaagcaa
    18241 taataaaaga aaaacaagta aataaaattg tatcaggatt accaaaaaat atgaatggaa
    18301 ctttaggtcc acaaggagaa aaagtaatca agttttgtga attggtaaaa gcagaaacag
    18361 gtattgatgt agagttttgg gatgagaggc tttctacagt agcggcagag aggtctttat
    18421 tagaagcaga tgttagtaga caaaaaagaa aaaaggttat agatatgttg gcagcagtaa
    18481 taattttgca aggatattta gattttaaaa taaattcata aaagaactat aaaattgaat
    18541 acttatgata tataatataa ataataaaaa taaaaataaa tttgaggtga atgacattat
    18601 ggaagaaaat ataataaatt taatagacga aaatggcgtt gaaagccaat ttgaaataat
    18661 attaacatta gaagcagaag gaaaagagta tgcaatatta atgcctttag atgatgaaga
    18721 agctgaagaa gctttaatct ttagaattga tgaggatgaa gaaggtgaaa tcttaatacc
    18781 tttggaaagt gatgaagagt atgaaactgt tgtagctgtg tatactgcta taatggaaga
    18841 agaaggttta aattacgacg aagatgaatc aaatggactt aactagataa gtaattaagt
    18901 ttgtatataa aaatcagata tttattatat ttgctttaat taaaccttat taattgtaaa
    18961 agcatttata gtatatatct gtttttttgt tttttattaa aaataccaaa aaatttatga
    19021 attttggtga ataatatatc ttttatgggt atatatttat attatgagaa taaattaaat
    19081 ttatttaaat gtcttgacaa aaaatgaatt atcaattaca atattattta ataaagatta
    19141 tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa tggatttatt aaaagacaag
    19201 ttaaaagaaa ctggttttaa gattacgcca caaagaagag caatagttga aatattgctt
    19261 aaacatgacc attcacatct aagtagtgag gaaatctatg atttagttag ggttgactgt
    19321 cctgaaatag gcttagctac tgtatataga acaatgcagc tattggatga aataggatta
    19381 atatcaaaac taaacttaga cgatggatgt ataagatatg agattagtct acataaagaa
    19441 gactgtcata atcatcatca tcttatttgt aaaaattgtg gaaagataat ggaagctaaa
    19501 gaagaccttt tagacaatat agaaaaagag atacaatcat tgtacaaatt taaaatactt
    19561 gatcatgatg ttaaatttta tggactatgt gacgagtgta atggagtaag cgattcagaa
    19621 gaataaactt ctattgctta atgtcgatgg aagaatagta tagaactttt atattctatg
    19681 ctattcttcc attttttatt ataaaccatc tataaaatgc aaatatacag ttttcaagat
    19741 acttatagtt ttaatattga aaatataatt aatcctataa gtacaaaaat gaatgatttt
    19801 tctatgaaat tactaaatgg tcctccaaca gttaaaaaac ctaaagaaat tttcttttcg
    19861 cttagcggat aaaaaattgg tattccttgc tttgtaaata aatctcctaa aaacatatga
    19921 cttgcataac caatcgtacc ataaaaagat aaattattaa tattagtgat taattctatc
    19981 tgttttagta aaaaatatac tattatagtt gcaaatatgc tatgagaaaa acttctgtgc
    20041 tttaaccagg gaaaaattga caacattaga caaaaaattg taaaatatat ttcaacttct
    20101 atgatgtata agcatatagc taatagtata aatattaatg atagaaaaac ttttcttaga
    20161 aatgtgtggg ttagttttgc ttcaataatt ataattgcaa caaatgtaat tattgcgctt
    20221 aaataaaaat tatcatttat atttattgaa ataaagaaaa taataatatt aacagcatat
    20281 ataaaaattc tatatatgta tttggataca ttgtttttta gaatgaaatt tgagaccata
    20341 gaattagatt tatccaaatc gggtagtaca gcaaaagtag ctgaaactaa aatatcaact
    20401 aaagatatag gaattttaaa tattaaagat gtttggatag ctgttaagat tcctattgca
    20461 caatgagttt taccacgcat aaatcctcct aaattatccg atattaaatc atataatatt
    20521 tagaggatat caaaataaaa taggtttgtc aaaataatac gttaaatgta ttttaaatat
    20581 agcttaaaat atgatacgaa ttatggtaca ataagaataa ctggtagcga gtgcgtgatt
    20641 tgatattatt tatgattgta gaacgtgtta aaaaattaaa tagacagaag gagatgatgc
    20701 aattgttcaa aaagaacacc aacaagataa aggtaatggc cttgggtgga cttaacgaag
    20761 ttggaaaaaa tatgactgtt gttgagtata aagatgaaat aattgttata gatgctggac
    20821 ttagttttcc agaggatgaa atgttaggag tagatatagt tataccagat ataacttatt
    20881 tagtaaaaaa tagggataaa ataaagggta tatttattac acatggacat gaagaccata
    20941 taggagctct tccatatata ttaaagaaaa taaatgtacc tgtatatggg gcaagactta
    21001 gtataggtct tatacaagta aaacttaaag aacataaaat gaataatgtt aagttaaatg
    21061 ttataggtcc tagacaagtt ataaagcttg ataatatgga agtagagttt ttgaaaaata
    21121 accatagtat accagatgct tactctattg caatacacac agaccaaggt ataatatacc
    21181 acacaggaga ttttaagatt gacttgactc ctattgatgg agatgttatg gatatgcata
    21241 gaatctgtga gttgagcaaa aaaggagttc ttttaatgct ggcagatagt actaatgcag
    21301 aaaaaccagg atttactatg tctgaaaaga ctgttggggt aggtcttgac gaattatttg
    21361 caaaaggaaa tggcagaaga attatagttg ctacctttgc atccaacata cacagattac
    21421 aacaaataat aaatactgct gaaaagttta atagaaaagt tgctatatca ggtcgctcaa
    21481 tggtcaatgt agttggagtt gcaaaagaat taggatacct agatatttct gatgacatgc
    21541 ttatagattt aaatgatata tgtaagtatg aagatagtga attagtgata ataacaactg
    21601 gttcacaagg tgaacctatg tcagctcttg ctagaatggc tttttcagag cacaaaaaag
    21661 tagaaataaa gagtggcgat ttagttataa tatctgctca tccaatacca ggaaatgaaa
    21721 aattaatatc aagagtaatc aatttcttat ttgaaaaagg agcagaagta gtatacagtg
    21781 atatagctga tatacacgtt tctggacatg cctgtcaaga agaattaaaa cttatacatg
    21841 cattagttag accaaagttt tttatgccag ctcatggtga atatagaatg ttaaaaagac
    21901 atgctgaaat agctgaacag cttggtatgg ataaagaaaa tatttttgtc atgcaaacag
    21961 gagatgtttt agaattagat aaaaattctg ctaaggtagc aaaccgtata caaacaggaa
    22021 acatattagt tgatggtcta ggagtaggcg atgtaggtaa tatagtttta agggatagaa
    22081 agcatttatc agaagatggt cttatgatag ttgtagttac tatttccaag gatgagggta
    22141 aggtcttagc aggtccagat ataatttcca gaggattcgt atatgtaaga gagtcagaag
    22201 atttaatgga tggagcaaag gatataatta agaatgttct aaatgaatgt gaagagaaaa
    22261 atattaaaga atgggcttat ttaaagaata atattaaaga aaacctaaaa gaatatttat
    22321 atcaaaaaac taaacgaaat ccaatgatac ttcctatcat aatggaagta taatataaaa
    22381 aaactataaa tttgaaaatt agacctcttg taatttttaa agtaatatca aaaatctaat
    22441 tgatattatc ttaaga
//
LOCUS       DAELJO010000049        22284 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELJO010000049 DAELJO010000000
VERSION     DAELJO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.049"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1125
                     /locus_tag="KPB88_001582"
     CDS             <1..1125
                     /locus_tag="KPB88_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0053864.1"
                     /translation="SWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSI
                     IKNFAVKLLCWIDYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILY
                     INPNKDILDLYPECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKP
                     NSKNTLNNTNLTSKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVIN
                     NEGYILTNLHVVNDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVK
                     VSKKPVRGQKIVAIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLD
                     MFGNLLGLISAGYDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1217..3091)
                     /gene="asnB"
                     /locus_tag="KPB88_001583"
     CDS             complement(1217..3091)
                     /gene="asnB"
                     /locus_tag="KPB88_001583"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0053865.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDRKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYVEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGTDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3294..3968
                     /locus_tag="KPB88_001584"
     CDS             3294..3968
                     /locus_tag="KPB88_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0053866.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4081..4617
                     /locus_tag="KPB88_001585"
     CDS             4081..4617
                     /locus_tag="KPB88_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0053867.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(4783..5403)
                     /locus_tag="KPB88_001586"
     CDS             complement(4783..5403)
                     /locus_tag="KPB88_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0053868.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(5717..7855)
                     /gene="vanT"
                     /locus_tag="KPB88_001587"
     CDS             complement(5717..7855)
                     /gene="vanT"
                     /locus_tag="KPB88_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0053869.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAIPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(7873..8679)
                     /locus_tag="KPB88_001588"
     CDS             complement(7873..8679)
                     /locus_tag="KPB88_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0053870.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(8676..9776)
                     /locus_tag="KPB88_001589"
     CDS             complement(8676..9776)
                     /locus_tag="KPB88_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG-Cd"
                     /protein_id="HBG0053871.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10037..11119)
                     /locus_tag="KPB88_001590"
     CDS             complement(10037..11119)
                     /locus_tag="KPB88_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0053872.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLISKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11169..11870)
                     /locus_tag="KPB88_001591"
     CDS             complement(11169..11870)
                     /locus_tag="KPB88_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0053873.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12304..14835)
                     /locus_tag="KPB88_001592"
     CDS             complement(12304..14835)
                     /locus_tag="KPB88_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0053874.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(14943..15560)
                     /locus_tag="KPB88_001593"
     CDS             complement(14943..15560)
                     /locus_tag="KPB88_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0053875.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(15921..16805)
                     /locus_tag="KPB88_001594"
     CDS             complement(15921..16805)
                     /locus_tag="KPB88_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0053876.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKV"
     gene            complement(16996..18246)
                     /locus_tag="KPB88_001595"
     CDS             complement(16996..18246)
                     /locus_tag="KPB88_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0053877.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(18468..19232)
                     /locus_tag="KPB88_001596"
     CDS             complement(18468..19232)
                     /locus_tag="KPB88_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053878.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19226..19951)
                     /locus_tag="KPB88_001597"
     CDS             complement(19226..19951)
                     /locus_tag="KPB88_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053879.1"
                     /translation="MDKNINNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(19953..20321)
                     /locus_tag="KPB88_001598"
     CDS             complement(19953..20321)
                     /locus_tag="KPB88_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0053880.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20497..22041)
                     /locus_tag="KPB88_001599"
     CDS             complement(20497..22041)
                     /locus_tag="KPB88_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0053881.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7659 a         3769 c         2332 g         8524 t
ORIGIN      
        1 agttgggatt tatataatat cgactttcca tgtaaaatag aaaacgacac cttaaattgg
       61 actaaaaaga ttgcatttaa aaatacttta gatttatttt ccacaagtat gccaaattgt
      121 acatcctcta tcatcaaaaa ctttgcagtt aaacttttgt gttggataga ctattttcta
      181 cctaaattat tttataacaa ggtgaagact actatttcac caaagatagt atactttgga
      241 aatattaaaa ggcaagaact ttttttctta tattttttat cacaacttgg gtgtgatatt
      301 ttatatataa atccaaataa agatattctt gatttatatc ctgaatgttc aaaattttct
      361 actctatttg aactttctag gaagactcca aatatattag aaataccttt tgaaaggcat
      421 attacaaata taaattacag tgaaaatacc aataagactt ctcctactac gaataaacct
      481 aactctaaaa atacacttaa taacacaaat ctaacatcta aaaaaattgt acaagatgag
      541 gaagtagaac tttcttatga agaccttgca aagttatcag cttctgttgt tatgattgtt
      601 gtgtgcaata acgaaaataa accgtttaag agcggttctg gagtagtaat aaataatgaa
      661 ggttatatac ttacgaatct tcatgtagtc aatgatgggt attcattttt agttagattt
      721 gaaaatgatg ataaagttta tacatcttac caaattataa aataccattc agattatgat
      781 ttagctgtga taaaggttga tagaaaatgc aagccaatac ctgttaaagt cagtaaaaaa
      841 cctgtgagag gtcaaaaaat agttgcaatt ggaagccctc taggtctttt taatacagtt
      901 tcagatggaa ttatttcagc ttttcgtgat tttgaaacgg ttcaaatgat acaatttaca
      961 gctccaatct ctagtggtag ttcaggagga gctcttttag atatgtttgg aaacttacta
     1021 ggattgattt ccgctggata tgatgatgga caaaacttaa atttagctgt tgaaagtagt
     1081 cttgtcaaaa tatttgccaa taattttatt gagatagtaa actaatcaat caaaattcta
     1141 cttatcctat tccataataa tcaagagggt gaaaacattg catgcttttg ccctctgatt
     1201 aatattgaaa aaagtattaa tactcgacat ttttatgctc aataaataat tgatgccata
     1261 gacaaaatgt aaatatagtc catatttttc tcgaattatc tctatgacct ttaagatgcg
     1321 tatctaaaag ttttattgca tattttttat ctataaattc atctacattt gaattataaa
     1381 tagtttcctt tacaatatcc cctagagaat cttttaacca aactcttatt ggagttggaa
     1441 atcctaattt tttcctttga acaacatgtt ctgggactat ttctctaaac gcctctctaa
     1501 gtaaaacttt tgtattattc ttatttattt tttgctccat tcttaaattt tttgcgacat
     1561 caaatacatc tttatataaa aatggtactc taagttcaac ctgctcagct gtagaaagtt
     1621 tgctaatttt ttgaagtata tcgccttgaa gccatgtatt cacatctata tgttgcattt
     1681 tacttatata atcgtaatta tttttttgtg catctctata caaattagac aacaggtatt
     1741 catatatatt cttttcctta tacttaaaaa agaatctttt tacttcgctg ttttcaaaaa
     1801 cttttgcatt tcctatatat cttttttcta atggtgttgt agctctataa agataatttc
     1861 ttccttttat gtttggcata agttctgaaa ctcttcctaa tatactttta atataagtag
     1921 gtgaattgac aactgatttc atagtactat actctttata tatattatat cctccaaata
     1981 attcatctgc tccttctcca gacaatgcta ctttaacatg tttacctgcc tctttaatta
     2041 agaaataaag acctacttct gaagggtctg ctatagggtc atccaaagaa taaattacat
     2101 tcggtaatga ttttatatat tcatcttgtg ttatattaat ctggatattt tctataccta
     2161 acttatcagc tgttttcttt gctacttcta attcactata tcctttgaca ctaaagcctg
     2221 cagaaaaaga ttttatattt ggattaattt gtgaagccac agtagttata atagatgaat
     2281 ctgttccacc agacaaaaat gttccaattt ctttctcctc ttccatatga gttactattg
     2341 aatctactac tatatttctt atatcatcct cattcacatc tctattgcct aagtgatttt
     2401 tagtataaaa aaattttgta ctacttctaa actcaagttt attatatctc tttaaagata
     2461 gtataccatt ttctacccta aaatagtgac ctgctggtat cagccttatt ccttgtatca
     2521 tagtatcttc aggaagtaca tattgaaatg aaaaataact ttgtaagctt ctttcattta
     2581 tatttaaatt tttcatatat tcaagtaaaa acttatactc tgaggaaaaa attattgcat
     2641 tttccttatt tatataataa agtggtttta tcccaaataa atctcttgct ccaaatatac
     2701 tttcattttc tctatcatag attataaagt tgaacattcc tttaatctta tgtacacaat
     2761 tcttaccaaa ttctacataa gatgctaata agacttcaat atcatcattt gtagtaaaat
     2821 tgtgtccttt tttaattaat tcttctttta tttcatgata gttatatata tttccattaa
     2881 aaacaataac ttttctatct ttttcaaatg gtttatttcc ataaatagca tcaattacat
     2941 taaatctttc aaaatcaaaa gttttttcct catccttata ctctattcta ctagaaagtc
     3001 ttgtattatt tacagattct tttattttaa atttaatatc ttctttttta gatatatcac
     3061 ctttataata aattgaaata ttgccgtcca tataatcaaa attcctttct gtcctttagt
     3121 cttattctat atttcatagt tttgtctttg gctttaaaac ttattcaatt taaaatgaaa
     3181 ttgctattaa attcttataa attatctgtt aatatttcat atttttttta tttctagaaa
     3241 tactatatta ggaggtgatt tttatgaaga aaaaaatatt aataccagtt attatgtctt
     3301 tatttataat ctcacagtgc ataacttcat ttgcttttac acctgaaaat aacaaattta
     3361 aggttaaacc attaccttat gcatatgatg cacttgaacc ttatatagat aaagaaacaa
     3421 tgaaactgca tcatgataag cattatcaag cttatgttga taaattaaat gctgctcttg
     3481 aaaaatatcc tgagctttat aattattctt tatgtgaatt attgcaaaat ttagattctt
     3541 tacctaaaga tattgctaca actgtaagaa ataatgcagg tggagcttat aatcataaat
     3601 tcttttttga tataatgacg ccagaaaaaa ccataccttc tgaatcttta aaagaagcta
     3661 ttgatagaga ctttggttct tttgaaaaat ttaagcaaga gttccaaaaa tctgctttag
     3721 atgtctttgg ttctggttgg gcttggcttg tagctactaa agatgggaaa ttatctatta
     3781 tgactactcc aaatcaggat agccctgtaa gtaaaaacct aactcctata ataggacttg
     3841 atgtttggga gcatgcttac tatttaaaat atcaaaatag aagaaatgaa tacattgaca
     3901 actggtttaa tgtagtaaat tggaatggtg ctttagaaaa ttacaaaaat ttaaaatctc
     3961 aagattaatt aaatattaaa atccttttga aaaaaactta tgtttgctat ataatataat
     4021 aaaagatagt ctaaatttac caatataatg tttatagtga cgattcagga ggatttttat
     4081 atgataaaag aaacacatga aaggggaggt tatatattcg cacttttgat actccccttt
     4141 ataaacaatc tatacctagt taaatacgat attgcatata aaatagtatt aattataatt
     4201 tatatttatt ttgcatattt aggttcttta tttccagata tagatatgag aggttcttac
     4261 ataagtaaaa aatttatgtt aatatacaaa ttatttggaa gtaggtttag acatagaggg
     4321 tttactcaca gtctaattgc cttattactt atttatagtt tttttaaatc tctgacaatc
     4381 ttcactaaca ataatatagt tttctcatgt ttgtccagtg ggtttataat aggatatcta
     4441 tctcacttgt gtcttgacct tattactaaa gaaggtattg aattgtttta tcccattacc
     4501 ataaacattt ctttactacc tataaaaact aactcaaaaa cagaaaaatt catatctaag
     4561 ctattaaatt tcatagttat ttttttgatt ggatatcaat tttatatttt attatagttg
     4621 tagaaaaaat aaataacaat attttgaact aagtataaat aagttttata tattttagtt
     4681 tacaaaagaa ctaaaaattt tgtaccttaa tacaaaaaat aaaacgtatc ttaaaataat
     4741 ttagtactaa atcattttaa gatacgtttt atttcgtaca agctaaatca ttttatcatc
     4801 tttatcattg tgatgttttt tatgatggaa atcataatta tgattttcaa gatttttatt
     4861 tactctattt ccaatcttcc atgacaaaaa caccaataat ataaatgatg ctatctttaa
     4921 aggacttgtt ataaaacctt ctatatcact tgccacgtaa cttataacta taaacattac
     4981 aaattttcct gccaacattg ctggcacaaa atcttttaaa tcttttttac caaaagctga
     5041 tgcgatagta actaaacaac caggcataaa tggacaggca tacgctataa ataataactt
     5101 aaatccctgt ttatccatcc aacttattgc tttttcagtt ttactatttc tcaacttatt
     5161 aaaaagctta ctatcattaa atctgcttat taataagaat aatgaagttg tcccaagtcc
     5221 tgagccaatc catgataaca aacatccaat aaataatcct tgtattgcag catttgctgt
     5281 aactatagca acaagtggta aggctggtat aaagctttct ataaatgcac ttaataagcc
     5341 gattattgtg gctaacaccc aataattttg agtcaaatca agtagttctt gtatatactg
     5401 catattgctt ttcccctttt ttaactttaa ctattattat atactctata tgtttattat
     5461 ataccaatct caaaaaatga atctaatatt aaaaagtaaa taatctatat aagtttacag
     5521 ttaaggtata attattttat tatatattat aacaaaaaag gtgatgtttc aaaataaaaa
     5581 tctgaaacat caccttttta taacttaaat tgtcatgtaa gttataaaaa atatatatta
     5641 gacataatat tactaaaaat gtctagtcag agtaaaataa attacacagt acaacaaata
     5701 ttactattta attgagttac ttctttataa catttaacct tcttcctact ctgctcaata
     5761 attcattcga tatactacat gagttatttg caacttcaga agcagataat tcagataaat
     5821 tatctcttcc tattattatt gccgtattac caacttctac attaggtata tctgtaatat
     5881 caacagcaag ttggtccatg caaattcgac ccactattgc tgctctacaa ccatttatta
     5941 ttacgtaact ttttccacag gataaatttc taggtatacc atcggcatag ccaatagata
     6001 atatagcaat tacgctatct ctgcttgcaa taaagactct tccatatcca aaactatcat
     6061 ctttttgtat ttttctaata agaattactt gtgattttaa ggataaaaca ggtcgtaaat
     6121 caagttgcaa tttagtcgta tcatttggag aacttaatat cccgtataat gcaattccaa
     6181 ttcttacata attacactta agattgggat aattaaaaaa accataacta ctttgaatat
     6241 gtactttagg aatttttata ccttttttta acagaaaatc aattaactta taaaaacatt
     6301 caatttgttt atttgtaaag tctacatctt catctttcag actatctgaa acacacaagt
     6361 gtgtaaatat gccacatata tctaagtatt ttaatttaaa tgcctttaaa attgacctca
     6421 catcattaac atcaaaaccc agtctgtgca tacctgtatc aattttaatg tgtgatttta
     6481 tgttgtattt ctgtttgttt aaagagatag catgattaag gtcaataaca ctctgtgtaa
     6541 gattgtattt atgaagttcc tttgccctta ttgggttagt atatccaaga atcaatattt
     6601 ctcctagtat tccatatcga cgtaaatcaa tcccctcatc tattgttgct actgcaaatg
     6661 cttttacacc tatttggttt gcacaagtcg ctacttcaaa agcactatga ccataggcat
     6721 tagcttttac aactgccatc aattcacaat cttctggtat agctttttgt atcatcttta
     6781 cattatgttc caaattattt aaatcaattt caatccaagc tctattggta tttaccttat
     6841 gttctccttt caagtctttt tttagaggtt taagaatcaa tataataatt atagaagcta
     6901 taaaagatgc tatacttacc actatataat gaataaggct attgtcaacc aagacagttt
     6961 gtattccgaa tattttagaa aacatacgaa taacaacaat catcatagga tgtataatat
     7021 aaataattaa tgatgatgtt cgcaataacc tagctcgaga acctttcaaa aatgttaagg
     7081 ctgtaaataa gaaatacata catggcaata acataatata catgctatca tgacgctgta
     7141 cccctagttt atgtaaaaac attccctcac ataacataag taagaatgat ataagaaatc
     7201 caatcaagct atttttagtt gacatataat tagatttatt agatattatt ccaccaagta
     7261 tgaaaaatac aggtgcaaaa aatataccat ttcgtgtata atctgatact tcaaaaatat
     7321 acccatataa attctttaat agtggtattt tttctaaaaa accataataa ctatcaccaa
     7381 acataccaat tacataaata acaatagtga ttactagtga gtactttaaa cctagttttt
     7441 ttacaagaaa gtatgcaata cttgcaccaa taattgatgc aggaagatac cataaatgat
     7501 ataaagtccc atcaaataca atgtccttaa taatattggg cactaaatta ttcatggaaa
     7561 aatatccagt gtaaatattt aagggaatat aaattacaat agctatcccg taaataaata
     7621 atacattttt tagaaaagat ttcagtttat tacaattata attatatgtt gaaattagaa
     7681 aaaagcctga tagcataaaa aagaatggca cagcaatacg agcaattata ctagttaaca
     7741 caaagtcagc cattttacta tatgataata atggagatgt atgaattgca attataagta
     7801 atgcagctat aatcctaaaa taatcaagcc ctgcataaga tttattccta atcattcttg
     7861 ttcctccatt tcttagtcct ttctatttct ccaaaatgtc acaataaaac catccttatt
     7921 gttacccgat atttgatata cacattgttc aggtatagag attctcactt cttctttagt
     7981 atggataggc acataaaaga tttctataat tttttcttta tcttctattt ttaaacaatt
     8041 tgtatacgaa aatctcttaa tatattctat atattcttca agagcgagat tcattttatt
     8101 gataatctca gaatgcggat atcccacata acgaaaatgc catggttctt gagatattcc
     8161 tgttatttct tcttttttac actgataacg ctctataaaa ccataatgta gtgctgcctt
     8221 acgaaactca ttacaaatac cattataagg aaattcaggg cagataaaat ctataacttc
     8281 tttctttaac cctaaatcaa tagcaagccc tgtctgatgt tcactgtgat ttggaagtgc
     8341 tacaaacttt ttagtaaagt cttttccatt gtcacgcaga gaacttgaat atatatcttg
     8401 ttgttcctct gcagtacgat atccacttac aggtactatt tcatttattc cacatatact
     8461 ttttaaaata ttttgtaaaa tgtttgtagc ttcacgtttc ataagaatac ttggaaacct
     8521 tgtatctaca ggaattaaat ctatatcttc agatgtttta actggtaaaa aagcatttac
     8581 tagaattaga ttcccatcat aaactttatc tttagtcaat aagatagtat ttgtcatttt
     8641 atcttcttta tttttatcta ataaaatatt attcatcatt ttatgtacac ctcaattatc
     8701 ttgtccaata tttcttcaaa agacaatcca atacctttca tcatatttgg ataacgacta
     8761 tgagaagtaa agccaggaat tgtattgact tcattaaaaa taatttcacc agatggagtt
     8821 aaaaacatat ctactctagc aaatccagaa catccaagag ttctataaat aatttttgct
     8881 gtctcttgta ttcttttctc acaatccaaa tcaattctag ctggcatatg aatttttgaa
     8941 cttttcaatg tatatttttc tgtataatca aaaaatcctt ttgacaattc tatttcatct
     9001 actcttccaa caagtaagtc attgtttcct aaaactgcac accctacctc aaaaccatca
     9061 atattttcct caattattac ttcattatca tgttcaaaag ccaagtctac tgctacttgt
     9121 aactcttctt ttttataaat tttactaata ccaaatgatg aacctgcacg cacaggtttt
     9181 ataaataatg gataagttaa gtcttttgac agttcagcca cattaaattt atctgagtat
     9241 tttagtaata ttgattttgg tactttaata ccttctgaat gaacaagttt atgtgatttg
     9301 tctttgtcca tacataatgc tgaagaaaga gtaccacatc caataacctg tatacctgcc
     9361 aattcaaata acccctgcac tgttccatct tctccatttt ttccatgcaa tacaggaaac
     9421 acaaaatcaa gtttagtgta tttaatatca ttttcacatg attcaatagc actatttgga
     9481 atatgattct gagaaacagc atcagaaatt aaaaattcaa taataccatt tttgacacgg
     9541 ttctgcgaaa caataactgg aattatatca gtatcccttt caaaccaagt gttattctgg
     9601 atattattat aatctccaat ataatgatac caattaccct cccttgtaat tccaattggt
     9661 agaatatcat atttttccgt attcaaattc tcaagtactg caaaagctga ttgtaatgat
     9721 acatcatatt ctgttgagtt tccaccaaat ataactgcaa tttttttatt attcatcttt
     9781 gattctcctt tgttgattta tttattttta tacaatctac agaaacatgt taaaatactt
     9841 tttctatttc aaatccaatc aaccttcact tacaattgca tctgttccac ataatatatt
     9901 ttcacatttt tctattgata taattactgt atcaaaataa aagcactctt gttttgatac
     9961 agtaattata tcaataacaa gagtgctctt tatttattta tcctaaagaa tttcctattt
    10021 ttttatactt aaattcttac aaatttccta caatcggaat aataacctta aaggtaatta
    10081 tattgttctc gctatgagct gttattgtcc cgttatgaag ctccactatt tctttagcta
    10141 tagcaagtcc caacccagct ctaccactgt ttgaattacg agatgtatcc aacctgtaaa
    10201 attgttcaaa aatacgctca agtttttcct ctggaatcgt attgctatga ttcataaatt
    10261 taagctttat ataatcatcc tcctgtacag ctgttatttc aatagtagta tcttcaaaac
    10321 tataataaac agcatttcgt aataagttat caaatacacg ttgcatttta ttaacatcac
    10381 accttatcat catatttggt gtaacattca gtgtatattt taaattctta ctaatcaaag
    10441 taggtttaaa ctcatatgtt agttgttcaa gcatacgagt taagcttact gtactgtatt
    10501 caagtgatat atttgataaa ttaaaccttg taatttcaaa gaattcatta attaaatctt
    10561 caagacgctc tgccttatcc aacgaaatag ataaatattt ttttcgcagt tcttcagaaa
    10621 tctgactttc atctcgaagt agtgtcagat accctataac tgatgtaagt ggtgttttta
    10681 aatcatgtgc aagataaaca ataagttcat tcttacgttg ttctgccatt tgtgtctcaa
    10741 atttacgttg ttcaagtata tgtttaattg agtttatttt cttttctatt gccaataact
    10801 ctggagataa agccacttct ccaacatctt ctttgataag agagtcaata ccttgattta
    10861 tttcactaaa atattttgta aaccaattta aataaacacg gaatattata aaaaacacaa
    10921 tcaccatagc tagtatcatg aagagctcca tgctattacg gaaaatctgc tgatataaat
    10981 tcaatgcagc tttatattcc atacgaaaag cattttcaaa gaatgatact atccagatag
    11041 aaaacttacc atacaatata aaagaatata aaatccatat gaaaataatg gctataaatg
    11101 ccattaaagc tgttctcata aaaattttat ttctcacttg agtaaagtca ggattgactt
    11161 taatattttt acttttcaat tgtataacct actccccata cagtttttat aaatttcggg
    11221 tttttagaag gttcatgaat tttttcacgt aatcgcccta tatgagccat aactgtgtta
    11281 ttattatcaa gatatttttc tccccataca gcttcaaaca attcttcaga aggaaccact
    11341 tttccttgat gttcacataa ataccaaaga atagaaaatt caatcggtgt taatgctact
    11401 tctttcccaa ataaactaca tttatgtgtt tctttattga taatcaatcc tttaatatca
    11461 tactcattaa caattatcga ctgttgttca taagaattat tatatcgcat atatcttctt
    11521 aattgtgtct ttactcttgc aacaacttcc aaagggttaa atggctttgt tatataatca
    11581 tcagcaccta tggttaaacc cattatcttg tctatatctt caattttagc tgttaacata
    11641 atgataggat aatagtattt ttctcgaatt ttctggcaaa tatgaaatcc atcaatatca
    11701 ggaagcataa tatctaaaat agccatatcc aaatttttag attcaataca ttctagagct
    11761 tccttacctg tataaaactt gtatactgta aaaccttcat tttttaaata tacttcaatt
    11821 aaatctgcta tttctttttc atcatccaca atcaaaatat tttcgttcat agattatttt
    11881 tcactctcct tattatcgag gtcatcattt ataatattgg taaatttaca tatcaaataa
    11941 ttacaagcat atttgtaact aatttttata agaaaagaac tactacatca gaatgtgttg
    12001 atgctactat tggaacacaa aaactgagat taattgttat tataaaaact cccctaacac
    12061 gtccttatta tcgcataaca ggtattaact ttcaataaaa atatctatca gtcaaacaca
    12121 attcaattta ttataccaga gacaatccat ataaaaaaca attaaaccta tgtatgtaac
    12181 cacagacatt actaaaattt ccatgttatt ttatataaaa attcaaaaaa ggagttgaat
    12241 ttaaaatttg attagaaaaa aatcaatttt gagacaactc ctttttattt taatgtattt
    12301 aaattatagt atatcagcct tcataatatc ctcaaaatca cctttttcag atattaatct
    12361 tatcaattta acagtacagc ttaaatcatt tataagtata gctccaacta atgaaccatc
    12421 tttcataaat aattttttat atattctttg accttcatct tttgatgaaa tttctttatc
    12481 aaaattttgt atattaccaa tactaaatac ttttacattc attgcatcta atgaaactgg
    12541 atatacttca ctttcaaatg tcaaattatc tccacttgca tttgcccctg ctatttttcc
    12601 catttctact gaagatggcc atattgctaa actaatattt cccacttgtg caacatctcc
    12661 acaagcatat atatctttaa tatttgtctg taatgtctta tctacaacaa tacctctatc
    12721 aatttttaca gatgtatcct gaaccatttg agtgtttgct cttacaccaa ttgaaaatac
    12781 taccatatca cagtcaacac tatctccatc tttaaataca actttttgag cttttccatc
    12841 accttcaata ccatcaactg ctacacccaa tcttacatct attcctgtat ctcttacaca
    12901 ttgttctaat attttactac cttctttatc aagttgtttt gatagtatgc tatccatggc
    12961 ttcaactact actacttcta atccttttag tctaaattcc catgcagctt caagtcccaa
    13021 taaaccgcca ccaatcacta caactttctt agatttttct agtgcttctt ttacactata
    13081 taaatctttt atatttctaa gagtaaacac attttctaaa tcatgaccct taattggtgg
    13141 tataaagttt ctacttcctg tagctattac aagcttatca aatttaatag ctccatcatt
    13201 cactataata gttttattca cttcatctaa tttatctaca ttcgttccta atattactac
    13261 tatattattt ttatcatacc aatctttatc ttccatgtag aaatctgaac caagttcttc
    13321 atttatacca tcagaaagtg caggtctata gtaagtcaac tcatcttcat tagaaatcat
    13381 agttatctta caagttttat ttctttttct tatagcatct gctgcataaa aaccagctgc
    13441 tccatttcct actattacga aatattcatc agtatctttt ctaaaagtaa cttcatcttc
    13501 ttcaacttct ataaattggt cttccttagc tccacataca gggcatacac taggagcact
    13561 atcaccttca aaaacttcat tacaaattac acatttaaat ttcttagttt tctttttact
    13621 tccaaaagta gaatttaact tatctaggaa tttacctgta taagaatgta atttatcaat
    13681 ctcttcattt gatggtttaa agttaacagc aaaaggtttt actgctgtca ttctaagctg
    13741 acttattctc tccatagcat tttctatacc ttctccactc catccatatg aaccaaatac
    13801 tgaagcaact tttccaccat gaagaactgg gtttaacgat attaatatat cccatatagg
    13861 ctttaacgca tctcctaata ttgttggtgt tccaaataat acaccttgtg atactgctat
    13921 ttttgccaaa atatcttctt gcttatgctc tattacatca aataaattta ctttatagtt
    13981 gctatttttt tcaatatatg cttttatagc ttctgccata atctttgtat atccatgtgc
    14041 agaaacataa caaattgtaa cttctttttc taactttatt tgttcattta cactccagtt
    14101 gtaataaagg tctataattt ttctaggatt ttcagttaat actggaccat gacctggaca
    14161 tacagtatct atatctaaat cttttatctt ttctatagca gttaccatag atggtttaaa
    14221 tggacccatt atacaatcat aatagtatct taaagcatcc aagtaatcct tttcttcatt
    14281 ttcatcaagt gtatttacta tcttatcatt actatagtga ctaccaaatg aatcacatgt
    14341 tacaagagtt ttatcctctt ttatatatgt gtatatggta tctggccaat gaagcatagg
    14401 tacagagaaa aattctaaag ttttgtcccc aattgataat gtatctccat ctgtaactgc
    14461 aacatattca aagtctttgt ttacgatttc cttaagatat ttaattgctg tttctgaagc
    14521 tactactttt gcatttttag ctaaatccaa aagcttttca acacttcctg catggtctgg
    14581 ttcagtatga cttacaacaa tatagtctat tttttcaaaa tctatattta aatcatttaa
    14641 tctttcaata taattgtcaa aatgcttatc tttaactgtc tcaaataaaa ctgttttttc
    14701 tgttcctttt aatacatatg aattataagt agtcccataa ggtgtataca taattatgtc
    14761 aaatactctt aaatcagggt caagtgaacc tacccaatat aaattttctt ttatttttag
    14821 tgatttcaat gacataccgt actcctccta aaatagttat ttagtctctt tataccctgc
    14881 ttgttatatt ttaaacaaaa tttattcaaa aaagaaatta ttttaaagat agtttaaatc
    14941 ttctaatctt ctttttctac cttcaataca gttccctttt tagaatctac ttttatgtca
    15001 tattcatttt tatctttatc tattcttatt ttataaaaag taacatctaa atctttgtct
    15061 aaactccact ctttaaccat tccattttgt tcttttaatg caatttccat agcttgttga
    15121 ggtttaatta tgtcatttaa atctaatttt tcttttctag caactccatt taaatcttct
    15181 gaatccaact tttctgtttt atcttctaat acatcttttg tattagcatc aattttcatc
    15241 ttatattcat tattatcatc tacaccttcc acagtataaa caaatttatt taaatctctc
    15301 tcaagactta aatctactat atcagcatcc ttgtatttat ctttaaatat gtttattgca
    15361 tctgtgtatg gcactttaaa atcttgttcc attaaatttt cattattttt ttcatctttt
    15421 aaagcattat ttgaagcatt tgcctccttt tttgtattat cttgtgcttg tcctgaatcc
    15481 ttagttgggc tcccactaca tgcagttaat aaacctgcta taagtatact taccactaat
    15541 aaatacgttt ttttacacat aaaaatcctc cttaaatata ttaatttatt ctatattatg
    15601 ccctctgaac cctttattta cccttttata ttaaaaattt ctcctatttt atcaatttta
    15661 aaactttata tttactaaaa ttaaagttac ataaaaatta tactgtctca aaatagaaac
    15721 aagcatcttt ttcattgttg aaataataaa aatatatttt atttcgtaca aaaagagtgc
    15781 ctcatgaact aaaaagttca ttttgagaca ctcccatttt ttatattttt acttacaaaa
    15841 taacagtact tattaatttt ttacaaacct tataagtata tctatatgat attcttaaat
    15901 ttaaacacta tattaaaaaa ttaaactttg tttaattctt ttaccgttaa atccatttta
    15961 ggatatggaa tcgtaattcc ttcttcgtca aacctaactt taacctcttc tagtaaatca
    16021 agataaattg tccaataatc ttctgtcttg caccaaactc tagttgcaaa tttaatagca
    16081 ttatctccat gttctactac tcctataaat ggttctggtt catttattat taatttatga
    16141 ttattaacaa tatctttaag tactcttcta acatgtagta tatcatcttc ataccctaca
    16201 ctaaaagtca aatcaactcg tctagtattc tttgctgaat agtttattag actatcattt
    16261 gatacagaac cattaggtat aagtatctgt ttattatctg gagtaaccaa ttgtgtataa
    16321 aaaagtccta tttgttcaac cgttcctcca tggcctgctg cttctacgta atctcctact
    16381 ttaaaaggtc taatcaatag tataataaat cctccagcaa aatttgatag actaccttgt
    16441 aaagccaaac ctattgctac ccctgctgat gctatcacag cagcaaaact ggacaaactc
    16501 attccccaat attccagaac aatgattata acggcaactt taagtacact caaaagaaga
    16561 gacttcaaaa accttcttaa agttaaatca atatctcttt tactaagtac taacataaca
    16621 tggtttacaa atttttttat tattttaaat ccaatagaaa gtataagtat acctattata
    16681 agttttactc cgcttgtagt agcccattct agcagacttt ccattatacc tcccatatcc
    16741 atcttattaa tgtctaaatt atttaaatct aacttgctta tatcatctat tttcgtcgta
    16801 tccataaaaa actccttacc ttatatttta gaaccaatat tatagtttaa agttcttatt
    16861 ttctatttaa tataatacaa taccatttgt acatagtaaa caagatttat attccaatta
    16921 tacatcttag gaaaatgcaa actatttgtt atatatatgc aaaatataat actaaattaa
    16981 actattaata aaacactata tagccttaaa atcatcattt tttaccccta tatgagatga
    17041 tactatagtt ataatactta gtactataaa tactacaaaa ccttttttat atccatcaac
    17101 tatattatat gaatttactt gtaaagagat agctataatc aaattaataa ttccacttcc
    17161 tataaattca aaagtattaa taacactagt tgccagtcca gaataaattt ctttattttt
    17221 atacttggca tcattaaaaa cctgtagatg tgacataact atacatccca taataaagaa
    17281 tgatattggt aaaactataa ttggtggttt tacctggtat acaactacaa ttacaatcca
    17341 tataaacaaa tctatcattg ccccaaattt tattatatta aatttgctag accttatttt
    17401 agcaaataca aaatccataa ttattgaacc aaatataaat ccatatgtaa aaaatgatac
    17461 tataaacgca gaaaaactct tactcacacc ataaacgtcc attatatatc ttactcccca
    17521 caagcttata aatgcacttg taagacccac aaaagagaac attatcattg aattatacca
    17581 tgttgatttg ttctttataa cagacttaat accatcaaca atattaacct tctctgaatt
    17641 ttcatagggt tcagtatcta cattgaatcc atactctttt ggtgtatctc taacaataat
    17701 atacataaaa catccaatta caacaccaat tataccgatt aataaaaatg aatttctcca
    17761 accaacaagt tcagacaaaa acacaagtgg aaaagtagca agaactccac ctaaatttcc
    17821 tacaaatgaa aatttagctg ttgcagaagc aaattctgac ttattaaacc atctcccttg
    17881 aatttttaaa atacaaagta atattactga tgttcctgcc cctacaatta cacgagaaat
    17941 atatgctaat tctatacttt gaatcaaacc aaataaaatt gaccctaaac ttgccattat
    18001 tattccaaga ctgctaattt ttcttgctcc atatttatca atcaaaattc ctgcaggtat
    18061 ttgcataaat gcatatgcat ataggcaaaa ggatgctata tttgaaattt gtatagaatt
    18121 aaaacctaac tcatttgcta aattgtctga aacaacagca gttgacatcc ttaaaaataa
    18181 tactaaaaca aatgaaaatg taattatacc ccaaacactc cacttagcct ttgtataact
    18241 tttcatatct tctccctcaa atccatatat ttttaaattt tcttgatata acttatataa
    18301 agtgtatagt tattcttaca aatttttaca cgcaatatca ttatacaaca tatgtatata
    18361 tttaactaga aataaaacac tattcatatt atatacatga attaactaca taaataataa
    18421 gagccatctt ttaacattac tcaaaagatg gctccatata aaaacattta ttctaaattt
    18481 aattttttat ctaatataaa ttttgtagcg aaaaataacg ctattgcaac tacaagacta
    18541 ccaagtattg caagcataaa actaggactc tgataaagat gatataccat atttgtatct
    18601 tcatttggta agatgcttcc aactgcatct ccaacaattg atattactat atttatagct
    18661 ataaaagaag caaatgccac tatgtttcta tgtttattga attttggcaa ttgtcccatt
    18721 gagattgatg tatataataa caaaataaat gatgaatacg aaataaacat tactactact
    18781 aataataaaa tatctaaaaa gttttcagaa atccatttaa aactagtgtt aaacaaatct
    18841 aaaatttcag gtagtaaaat tccagaagta ccaaaaagca taagaactcc aaaagtgaat
    18901 actgcaacta taaaactaag tacagcgtat ataagtgctg ttatacattt agataatata
    18961 agtgaactag tgctaacagg aagagtaaac attaaataac cttcatcccc caaaagattt
    19021 tttctaaacc tttgaattgt aactacaata gttaaaactc ctaatgccat aaaaagactt
    19081 gttaatacta gtattcctat tccctgtacc tgaagtatat ttgtattcat aaatattcca
    19141 ttaaatacag ctactatcaa tatggctata tataacggta taaatattct tccacttgct
    19201 tttaactcat attttaataa tttacctaac atttaaattc ctccctaaat aatgcatcta
    19261 tagattttcc tttttcttct cttattgctt caacgtctcc ttgtaacact atttctcctt
    19321 ttgatatgaa tattatctca tcacatatgt tttctatttc acttattaag tgggtagcaa
    19381 tcagtaaagt actatcttca gaataatttt ttaatatagt cttaagtata tatgaccttg
    19441 cagctgggtc aacaccacct ataggttcat ccaatatata tatgctcgca tttcttgaca
    19501 ttactaaaat aagttgaact ttttctttag ttcctttaga cattgtcttt agcttttcat
    19561 ttacatcaat atctaagctt ttaatcatct catttgctct tctaacatca aaatcactat
    19621 aaaaatcata gaaaaactta agtaaatctg ataccttcat ccaatcattt aaataagttc
    19681 tttctggtaa atatgaaact atttttttag tatctatgga tggtttcatt ccatttatca
    19741 taatttcacc attatcaggc tgtaataatc cattcattag cttaatcatt gtacttttac
    19801 cacttccatt aggtcctaac agtccgacta tttttccctt tggtatattt aaatcaatat
    19861 tttttaaaac attcttagtt ccataactct tgtttacacc tttaaattca actattccat
    19921 cttgagaaac attgtttata tttttatcca ttttataatt ctcctttcag attttctgta
    19981 ataagtttta gcatttcatc tctatcatat ccaagctttt ctaaggttac ttttaatgta
    20041 tttatctcca cattagctat ttcctctttc atagctttta ttttttcctt atcatctgtt
    20101 acaaatcttc ctttagttct ctgactataa actaaacctt gtctttcaag ttctgttaga
    20161 gccttttgca ttgtatttgg attaacttcc gcatcttctg ctagtgctct cactgtttct
    20221 aatttagatc ctattttaat ttcccctgaa attattttta actttaaatg ttctactaat
    20281 tgtatatata taggtttgtt attatcaagt tcccattcca tgttcatgcc tccttattat
    20341 attattgtat taagttgtta atacaataat ataataatac aaagtatatg tcaattactt
    20401 tttagtaaaa gtataaaaaa ttttacaaaa aaaatgaaca ctacattaga gtccattttt
    20461 aacacaattt attaaattct ttatattaca gattaattac cctcttacca agttagatac
    20521 aattaaatgt tcaaattctt caattggcat tggctttcca aacaaatatc cttgtgccat
    20581 atcacacttt acttctttta aaagtttaaa ctgttcttct gtctcaactc cttctgatat
    20641 gactttcata cccaactctt ttgccatttc tattacttta gtaacaatta ttttgcttct
    20701 tttactatta taagtttcca taataaattt ttggtcaagt tttaagatat ccataggaat
    20761 ttcttttaac atatttaatg atgagtaccc tgaaccaaaa tcatccatag atattaaaaa
    20821 tcctattttt tttaaattat tcattatatc aattaaaata tcaaaattat caaatatgat
    20881 actctccgtt aattcaagtt ctatatactt tgcaggtata ttgtatttat caattatatt
    20941 tttgtatgtt tctataaaat tattgcaata taaatgtatc cttgaaacat taactgaaac
    21001 agtaactgga tttattcctg catcaatcca ttttctaagg tatatacata cttcttcaag
    21061 cacatataaa tctaaattta taacaaaacc gtttctttca aaaaatggga taaattcatt
    21121 tggaggtatt agccctttat caggacgttt ccacctaaca agtgcttctg aaccttgtat
    21181 ctctcctgta ttaagttcta tcttaggttg taagtatata ataaattctt tattttctag
    21241 acttgaaaac attgtatttt ccatctcttt ttcttttatc tcttggtcat gaatcttatt
    21301 atcataaaat gcatatgaac ttttatgtcc tccctttgcc attttatgag gtatattagc
    21361 cctatctatg attgagttga tatctctatc ttctggaagt ataaaataga ttccacaact
    21421 taaaactaac ttatattttt cttcttgatt attattaaac aagtctaatt cttcaaatat
    21481 agtttttaac ctctttgtta tatcttcttc ttctatatat ttcataatta taataaaatt
    21541 atccgcagat attcttgcaa acatttcctc ttcaaatata ttcctcttaa taatctttgt
    21601 taaattacgc aaaatttcat tcccaacttc atatccaaat aaatcattta tatatttaaa
    21661 tttatcaata tctatatata ataaagcata ctttacttga gtatttttac atagtatttt
    21721 gttagcatca attacaaatt tatctatagt atttgctcct gtaagattat ctgtatatgc
    21781 tatcctttgc aatttaattt taaaatttaa tttatcatat attatataga atataagtat
    21841 tacacatatt atagccataa taagtattat ttgtattgca tgtttgctaa taagctgatt
    21901 attagacaca atagactcaa agttatttat ggagttgttt ttatagagtt tgcataaaga
    21961 atagactggt gttgtgtatg taatttgtaa aattatacaa ataaataaaa aaaacatcaa
    22021 ttttttttgt aaattcctca taaaaatttc ctcccattaa aacacctcta ttttattagc
    22081 aaataagtac atctcaaaag tttccatttc ttgtaaaatg gatagtttat atctacttta
    22141 attttgtaaa attttctgct actataatta tagaatgtag tatattcatt gtcttaacta
    22201 ttgatttata tatttcatat aggtataata gaaaataaat tggattattt tggcatataa
    22261 ttatttatta aaaggtgatt attt
//
LOCUS       DAELJO010000050        21792 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELJO010000050 DAELJO010000000
VERSION     DAELJO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.050"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(327..1760)
                     /locus_tag="KPB88_001600"
     CDS             complement(327..1760)
                     /locus_tag="KPB88_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0053882.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(1776..3200)
                     /gene="ascB"
                     /locus_tag="KPB88_001601"
     CDS             complement(1776..3200)
                     /gene="ascB"
                     /locus_tag="KPB88_001601"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0053883.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNNGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(3287..5203)
                     /locus_tag="KPB88_001602"
     CDS             complement(3287..5203)
                     /locus_tag="KPB88_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0053884.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQELITVLA"
     gene            complement(5277..6137)
                     /locus_tag="KPB88_001603"
     CDS             complement(5277..6137)
                     /locus_tag="KPB88_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0053885.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(6488..7675)
                     /locus_tag="KPB88_001604"
     CDS             complement(6488..7675)
                     /locus_tag="KPB88_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0053886.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(7689..9080)
                     /locus_tag="KPB88_001605"
     CDS             complement(7689..9080)
                     /locus_tag="KPB88_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG0053887.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(9675..11375)
                     /locus_tag="KPB88_001606"
     CDS             complement(9675..11375)
                     /locus_tag="KPB88_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0053888.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(11347..12750)
                     /locus_tag="KPB88_001607"
     CDS             complement(11347..12750)
                     /locus_tag="KPB88_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0053889.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEDVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(13015..13506)
                     /locus_tag="KPB88_001608"
     CDS             complement(13015..13506)
                     /locus_tag="KPB88_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0053890.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(13532..14344)
                     /locus_tag="KPB88_001609"
     CDS             complement(13532..14344)
                     /locus_tag="KPB88_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0053891.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVIFNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(14357..15508)
                     /locus_tag="KPB88_001610"
     CDS             complement(14357..15508)
                     /locus_tag="KPB88_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0053892.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARILNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            15778..16419
                     /locus_tag="KPB88_001611"
     CDS             15778..16419
                     /locus_tag="KPB88_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG0053893.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNIFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            16445..17989
                     /locus_tag="KPB88_001612"
     CDS             16445..17989
                     /locus_tag="KPB88_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0053894.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(18189..18815)
                     /locus_tag="KPB88_001613"
     CDS             complement(18189..18815)
                     /locus_tag="KPB88_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0053895.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(18950..19666)
                     /locus_tag="KPB88_001614"
     CDS             complement(18950..19666)
                     /locus_tag="KPB88_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG0053896.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            20092..21306
                     /locus_tag="KPB88_001615"
     CDS             20092..21306
                     /locus_tag="KPB88_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0053897.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT         7115 a         3914 c         2433 g         8330 t
ORIGIN      
        1 tttatatccc taaatatcat agttgcaata tctatcatat ttcttaaact tctataaagc
       61 aaaaattgtg ccaaattaat aattttctta attttattct aaagttcctc aatagtattt
      121 acagacttag cacttatagc ttataaatct ttatattatt aatctatttt tataggttaa
      181 ttgtacaaat catatatcac ttttacccat taaatgcatt ataaaaaaga tggaataacg
      241 attcgttact ccaccttttt tgtaataaaa aattgtatat ataaacacta tttactactc
      301 ttctaagtac aaacagtatc tacatactat aacctttcac cattagattc tattacttgt
      361 ttataccagt caaaactctt tttcttaatc ctagacaagt ctccacttcc atcatcatac
      421 ttatccacgt atataaaccc atatctcttg gccatctcac cagtagatgc acttactaaa
      481 tctatacatc cccatggagt atatccaaat aaatcaacac catcttctac agcttccttc
      541 atttgctcta tatgtgcttt taaatactct atacgatagt catcatttat cactccatta
      601 gattcttttt tatcataagc tcctaaacca ttttcaacta ccatcatagg tattctatat
      661 ctatcataaa tttcatttag tgcatatcta agcccttcag ggtctatttg ccatccccat
      721 tcactagact taagatatgg atttttcact ccacctaata tgttgccact tgtattatct
      781 tctttagaat cactacttac acatgtagac atataataac taaatgtata aaaatctaca
      841 caaccttctt ttaaaatttc taaatctcct ttttccattt ttatttcaat attattttct
      901 ccaaaatatc tattcatata agttggatat tcgccacgca cttgtacatc tccacagaaa
      961 taattcatta ctctactatc tctttgagtt ttaactgcat catctggatt acaagtataa
     1021 ggatacattg ttgcaaatat ttgcatacat ccaacttgat aatttgaatc tatttcatga
     1081 gctaacttaa cagccttagc acttgctaca aattgatgat gtaacccttg aaaacgaagt
     1141 tgaggtatat ctacttggtt tataaaatct gtagtccctt catttaatat tccttgtgat
     1201 aagtagcctc ccataggcat tgtagctgaa tttatttcat taaatgtcag ccaatatttt
     1261 acttttcctt tatatctttt aaatattgtt tcacagtaat tcatatacaa gtctattaca
     1321 tctcttgaaa cccatccatt gtatttctct gttattgcaa atggtatttc atagtgagat
     1381 attgttatta atggttctat attatatttt aaacattcat ctatcacatt ttcataaaat
     1441 ttaagtcctg cttcatttgg ttctttctcc attccagttg gaaatattct agtccatgct
     1501 attgaaaatc taaatacctt aaatcccatt tctgcaaata aagctatatc ttctttgtat
     1561 ctgtgataaa aatctattgc ttcatgactt ggataaaatg ttcctttttc tataactctc
     1621 gttattcttt tacttacttt ttgagaacct ccagtacaca tatctgaaca agaaggacct
     1681 ttcccatcta aattccatgc tccttcacat tgatttgctg ctgttgctcc accccataaa
     1741 aaatcttttt taaatgacat acctatatcc tcctattata attcttcacc atttgattct
     1801 attacttttt tataccaggc aaaactcttc tttttactgc gttttaaagt tccatttcca
     1861 ttgttatctt tatccacata tataaatcca tagcgcttct tcatttctcc agtacttgca
     1921 cttactaagt caatacatcc ccatggtgtg tacccccata aatcaacacc atctaactca
     1981 acagcatcta tcatggcttt tatatgagca cgtaaataat caattctata atcatcattt
     2041 atatttccat tttcatctgg attatctact gctcctagtc cattttcaac tataaataat
     2101 ggtttttgat aacggtcata taaagagttc atagttattc taagtcctaa tgggtctatt
     2161 tgccatcccc actcacttgc atctagatat ggatttttaa gacttgcaaa aacatttcct
     2221 tctgttaaaa tatttacttt tgggtctgca cttgtaagtc ttgatgagta atatgagaac
     2281 gaaataaaat ctactgtgtt ctctttaagt atctttttat catcttcttc catcactata
     2341 tttattcctt ttctctccaa ttcttttaat gcatatgttg gatattctcc acgagattgt
     2401 acatctataa aaaagtaatt ttccctatct ttttctatag atttccatac atctagtggg
     2461 ttacaagtat taggataagt atttcctgct gctaacatac accctatttt aaattcttga
     2521 tttatgttat gtgctagttt agtaactaac gcactcgcaa ctaattgatg atgtgcagct
     2581 tggtatttta tttgttcttc attttctccc tcttcaatat agagacctgc acctataaat
     2641 ggtaaatgaa gtatcatgtt tatttcatta aatgttagcc aatactttac cttaccttta
     2701 aaccttgtaa aaataacttc acagtatttt acatataagt ctactagctt acgatttctc
     2761 catgctccat atttttttac caaatacatg ggaacatcaa aatgacagat tgttactact
     2821 ggttctattc catactttaa acactcatca aataaatctt catagaattt tagtccctct
     2881 tcatttggaa catcctcaac tcctgttgga aatatcctag tccatgctat tgacatacga
     2941 tagcatttaa atcccatctc agcaaataat tttatatctt ctttataatt gtgatacata
     3001 tctattgctt catgagctgg gtaatagtgt tcatcatcac attcaaacat tttcattttc
     3061 ccagccaaaa ctggaaatct atcttttcct tgcggtgtaa cgtcaacatt agctagacct
     3121 ctaccatctt ctttatatcc accttcacat tgatttgctg ctgttgctcc accccataaa
     3181 aaatcttttc taagtgccat aattctacct ccaataattt atttatagta actttatttt
     3241 aaaataagaa tacatatata taattcttaa ttaatagaac tctcttttac gctaaaacag
     3301 ttatcaattc ctgactatta tttacatgct tttgtgtatt ttctacaata tccacataat
     3361 cagaataatt agtaacaact actggtgttt ctaagcaata tccttctttt gaaatacttt
     3421 ctatgtcaaa agttattaat gtctgacctt ttttaatctt atccccttga ttaacaaaag
     3481 atttgaaata tttaccttcc aactgaatag tatttaaccc tatatgtatc aatagttcac
     3541 aaccattatc agatataatt cctattgcat gttttgttgg aaataaagtc attacagtcc
     3601 catcaaatgg tgccactact tttccttctt taggtgttat tgctacacct tttcccaata
     3661 tacctgttga aaacgctgca tcttgtatct gctccaaagc ctttacttca ccttttattg
     3721 ggctatatac tacttctctt ttgttttttt ctataacgcc ttgcccatta tcatctttag
     3781 aaatttctac tatagcatca tctttccaaa aaaataatgt taaaacaaat cctactacaa
     3841 tagctactat tattgctatt atagttgcat aaacaccttt catatctcca gttgttggat
     3901 ttatataatt aggtatacca aatattccaa gtccacccat tacataactt tttacattca
     3961 ttatccccat tacagctcct gccacaccac cacctacaca gctatatatg aatggtgttt
     4021 ttcttggtaa agttattcca tatattgcag gctctgttac accacatatc cctgaaataa
     4081 ctgcaggtat acacagctct tttatcttct tatcttttaa tttaaagtac attgctgcta
     4141 ctactgctgt ttgtgcaaaa gatggtacaa atgctcccac taatattgtg tcaaacccca
     4201 tagtcataag gtttgacata gctattggta ctaatcccca atgtagacca aatattacaa
     4261 gcacttgcca caataaacca gttaatgcac cacataaaat tggtgaaaag ttaaataaag
     4321 ctaaaaatcc atttccaact atattagaag cataggtagc tataggccct ataaccataa
     4381 atcccaatgg taaagaaact aataaaactg ccattggtac aaagaaattc tttactacat
     4441 ctggtattat tttagcaaac actttctcca acttagctgc taataatact attaaaatta
     4501 ctggtactac tgttgaagtg tagttcattg ctattactgg taatcctaag aaagttatat
     4561 atactggtga ttcaaatatt gttccagtaa atagattgta taaaggttga cctgtagatg
     4621 ctagagtatc taattgaata cctgaatagc ataaaactgc acctataagc attccaataa
     4681 atggttttaa tttgaatttc ttagcagctg tatatccaag aattattggc atatactgga
     4741 acattgcatc tccaattgca tttaacataa tatatgcacc atcagtttct ccatacaaac
     4801 ccaatgctgt aaatactgca ttaaatcctt ttatcatacc agcagcacac agaactccta
     4861 atattggttg aaaaactcct gatattatgt ctataaactt attaagtaat ccttttggcg
     4921 catctgaatc tgaattatct attgtatctc ctcctaatcc agccaaatcg cacacaactt
     4981 cataaacatc tggaacatga ttaccaataa ctacttggta ttgacctgca cttttcatta
     5041 cagtaactat accatccatt ttttttagca catcatcatt tgctattgac tcatctttta
     5101 atttaaaacg tagcctcgtt atacagtgag ataaactgct aatattatcc tttcctccta
     5161 cattcgcaat gatttctttt gatagttgtt catactttct cattttttct cctccatttt
     5221 ctttttgaaa tatgaaggtt tggccaactt aatgtcacca cccaatatat ataaacctaa
     5281 ttattaggat ttatatctga cttaatactt ttacttacca ctctttcaac gtgaatagtt
     5341 aagtataatt tttcctcatc tgttaaatcg tagttgtatt ttttatgtat aaattcatcc
     5401 aacttttcta tgaatttata agcattttta tatttaactt ttattacatt taataaatca
     5461 tcttcattat tttcatgtaa attattacta acaattcgtt ctgcaaaata ctttaagtga
     5521 gtaataaatc tatgataata aactgaatct tcatcaaata ctattctgta atggtattta
     5581 accatatttg aaatttcttg tattatttgt gtaatctcat atatagtttt tatactttga
     5641 tccatttgtg cattaactat atgcagtgct ataaatccag cttcatcttc tgatagtcta
     5701 atttcaaatt ttttttctat ataatccaat gcctctaacc ctattttaaa ttcactctta
     5761 taaaatcttt ttatatccca aagtaataca ttttttacac ttactccttc acgagctctt
     5821 tctattgcag tatacatatg gtctgttaaa gaaatataaa tactatcatt taatcttttc
     5881 cctagttttt ctttagcata taaaattatt tcatctgata attccatata tcttatagga
     5941 atatctgcta ttagttcttt aaatttatca gatatatttg ggtctgaaag tttgaatatc
     6001 ttatcaattt tatcctttga tatatgctct cctgtacgtt ttttataagc taaacctctc
     6061 cccataacta ctatttcctt atgattttca tcagttgtaa taattacatt attattgagt
     6121 attttttcaa taatcattgt gatgccccct ttattagcat taagttaaaa taaatctttt
     6181 ttaattattt agaatattta aaatgtaatt tattacataa aaaaaaacct atttattaca
     6241 cattaaatag actcccatga gtcctgagta ataaataggt ttagcttgta tttaaacaat
     6301 cactatccgt ttgtatttta ttttaattat tatttattat atgttagcaa atttattttt
     6361 tagtgtcaac tttttctcaa tattttaatt ttaaataaat tatataaatt gaatcttcaa
     6421 agtactattt ttatcaataa acaaaataaa aaaggtatta ctttctttaa gtaataccca
     6481 tttattttta atttaagtgg tttttcttaa tctcatcaag tagtccatct tctaaataaa
     6541 tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca taagcttcct
     6601 cagaatttgc ataatttaga aattcttttt catgaacata taccattgga cttatatcta
     6661 aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata tctgtagaac
     6721 ctactggacc atctttacct tctaatatat ctgttatacc tgcctcaatc aaattattct
     6781 ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt tcaaagtatc
     6841 tataagaaag cttagctcct gtcattaact ctgcaccttt tgcacagttt ataacttttt
     6901 tagttacttc atctaaataa cttctttcag cagctcttac gaaaaactta caggctgctt
     6961 tttctggaac tatatttgga gcctctcctc catttgaaac tataccatgt attctaacat
     7021 ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt actgcatcta
     7081 gtgcattgac tcccatatga gggtgactag ctgcatgtgc tgatacccct tcaaaactaa
     7141 actctaatga atctatcgcc aaagccttac agtttatatt attaaaagct tctatatgca
     7201 tttgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca cattttcctc
     7261 ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa ttttctttta
     7321 acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta tgtccacaag
     7381 catgagctgg ttctttattt tctccatatc caggaagtgc atcatactca gctaaaaatg
     7441 ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc atattacaat
     7501 atggataaac tgtatcaaat ccatattcct tcattttttc tactaagtat tttgatgata
     7561 tgtattcttc ttcccctaat tcaggattgt taaatataaa attacaaact tcttcaattt
     7621 cttttccaag ttcattctct atctcataag ctctcttctt tatactctct atcataattt
     7681 acctcctact agaatcctag atataatcct attgtcaaaa atgccattac aagaattgtt
     7741 aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat attggctact
     7801 gctagtgctc ccattaaaac acctgatgta ggtgttatca agttagttac acctatacca
     7861 aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc aagtggtgca
     7921 agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa tgctattaat
     7981 ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt taataaattt
     8041 gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat tacgattcca
     8101 cgtgcaaatg ctatacacaa tcctgctgta attaaatctc ctgctccact tataaatgaa
     8161 ttaactatct catcttgttt aagccctgat gctattcctg atattatacc tatagctgta
     8221 aatatcattg caatttcagt tatgtaccat tctagtctta aaactccata tatcataatc
     8281 gccataccta ttgtgaaaat ggctattacc atggcttctc tttttgtaaa ttcctttata
     8341 tttgtatcac tattaaccat taattcttga tttagtgata tatcgtgtac taaagatttt
     8401 ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga tatagccata
     8461 tatattataa acataacaac tctaaactct attccagaac ctggtactat ttgtgataat
     8521 gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc agctgttgct
     8581 cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt cataagtgga
     8641 ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt cccacctagt
     8701 ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat tttatttaca
     8761 gtatgagcta taacagcttc tatagctcct gttttattaa ctatcccaaa agctcctcca
     8821 actattaaaa caaatccaac tacttcagct gcatctataa acccgtttat aggtgctgta
     8881 aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc agctacaaca
     8941 actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat ccaagtaaga
     9001 attgccatta acactattag caaaaatatt attgtaagtg tctgtggcat agtaaacttt
     9061 ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata ttttattatt
     9121 gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata catgttgagc
     9181 taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag atagctctcc
     9241 atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag tcccagcact
     9301 agcttcggac actagcactt cggcacttac tccttttata ttgcctcatt ggtttccaac
     9361 ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgtttgt tagatgaggt
     9421 aattcttatt aatttttatt aattcaaagt aattgtagca taatatgtcg agattgtaaa
     9481 tattttcagt ataataattc ttactttatg atttcattcc atttttcaca aataattttt
     9541 tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt ccacaaattt
     9601 taaattatta taataccttt ataataaaaa ataaaagcta tgtaaactta tcaatttaca
     9661 cagctttata tacattaatc tactccaagt acagctttaa gctttgatgc tattgtaata
     9721 actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc acttgcaccc
     9781 aattcattta cccagaagcc atcttcatag aatccatcaa ttgaatttag ctctacccta
     9841 agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa accttctatt
     9901 atagaatgta caagctttgt atctttatca tctacttcct cttctactgc attgttgtat
     9961 gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact ataaagctta
    10021 acttcttctg aacttcttcc tggtgttttt atatcaaact tcttgattgc ccatgtaaat
    10081 attatataag taactataaa aaatattggt atcaaaaata ataatggtaa cgcatccact
    10141 ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat tatagaaaca
    10201 ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc tacaaaatac
    10261 aataatggtg caacaaatag aaatgtatac tctattggtt ccgttacccc taccaacatg
    10321 gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc attatcagcc
    10381 aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa taaacgacct
    10441 gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg agctaatctt
    10501 atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc tactcttgta
    10561 tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc tttatccatt
    10621 gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc aagtttagat
    10681 aatgccaacc ctactggtgg ccatataaag tacattgctg tacccaatac tattgatgct
    10741 ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc actaggaagt
    10801 tctatgtcaa caaatttgtt atgtactgca ctagctacaa atccagctat tatagctcca
    10861 aatatactac aatcatatga aaagattcct aagaaagaac catataaaga acttcgacta
    10921 tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac tgtatctgaa
    10981 tttataccat ttgctgctaa caatgtactt ataactgtgt gcattcctat aaataaaaca
    11041 attcctccta atgcacccca tcctttttcc tttttagata atccaaaggc tatacctgta
    11101 acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat tactttaaag
    11161 aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc agaaacgttt
    11221 tccatatttg caaaagctga ccctaatcct ataaatactc ctgctacaac aagtaatata
    11281 acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt attatttcct
    11341 ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta ttgctttgta
    11401 taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat ccccctctat
    11461 tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag ctccttttgt
    11521 aaaaagttcg gcttcttttc caaaaccttg tttatatgca tttgataatt tatttattaa
    11581 ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa ctattaaatt
    11641 ttcttttttt ccaatttcat taatcctatt agttataaac tcccaactta tacccttagg
    11701 atatctaaag tctattttca tattcaattc atttttctct aagaagatac tcattataca
    11761 tattgtagaa actcccatat ctacatcttc tttatatatc cctaattttt ttccatgatt
    11821 atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat catttagttt
    11881 tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc tgtgtggatt
    11941 tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttctt ctagttcttc
    12001 tatctctgta taataagcta atgattctac taaatctttc ttatcattag ttttaaaaac
    12061 agcctcaatt ttgtcacata caaatccatc ttctttgttt gactttatat caactaaatt
    12121 gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata aaataccttt
    12181 ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg gctgttcatt
    12241 gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac ccactatcaa
    12301 tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg catataaaat
    12361 ccctatcaaa ggtcctttgt tgtcattaac tcctctacca taaagatagt tatctttttg
    12421 acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca ccacatctat
    12481 atgattaagc acccctacta cgtcttctcc ttctccatat tctatatgaa ttgcatatcc
    12541 atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct tatcgaaggc
    12601 atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat ctctaattga
    12661 aggtatttca actagacttc taatatcatt tatgatattt tcttgattgg attttaaata
    12721 atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct ttatattaat
    12781 tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt ttttcaatat
    12841 ttcaaatact tctctaagtt accataatga tacggtaaac aatcaacaga ttatgacaaa
    12901 atcatactat acctattact aatattttac cttatctatt acgtcttttt taatgtaagt
    12961 ttattccaaa aaatacataa aaaaagatgt agccttagct acatcttctt atatctattc
    13021 aacttccatt attatgctat taatatctac atttccaggt tctatcctat cttttactgt
    13081 tctcttaaca gaatcagcat catccattat aactacagga gttattatat ttaaatcatt
    13141 tgacgttata aaatctaaat ctacttccat tattaaatct ttagctttta ccttatctcc
    13201 tgcctttacc ttaacatcaa acggctttcc ttctagtgac attgtctcta aacctacatg
    13261 tattaatata tttgtattat cattactctt aatcattatt gcatgctttg tatcaaatac
    13321 attgactatt tcaccatcaa caggagaata taacttaccc tcacttggta ttattgctac
    13381 tccatctccc atcatctttt gggcaaaaac tgcatctgga acatcttcta agttcactaa
    13441 atttccctta aaaggactat atattttctc aatcttattt tcttttttct taaatatatt
    13501 aaacataaca cactctccac attataaaat tttatgataa tatttttctt aaagcttttg
    13561 ctacaccatc atttacatta gtatcagtta caaaatctgc catagatttt acttcatctg
    13621 tagcatttcc catagcaata ccaagacctg caaatttaat catggatata tcattttcat
    13681 tatcacctat acaaataact tcttcgctat ctatacctaa tatatcacaa aattttttaa
    13741 ctgcaatacc ttttgaagta cctttgctca taacttcaaa atttatcttt ccagaactta
    13801 ctacttctaa atcttcttgt tttttaaatt cttcctttgc ttctctcaaa gaatctacgt
    13861 tttctgagaa aagtatacat tttaaaattt cattatcagc tttttctaat aaagtttcac
    13921 aacttgcatt ttctattatt tcaattctgt cttcttcttt atttttacta tttattaatc
    13981 tatatggatg ttcttcacct atttcattgt tgaatataac tattttacag cctttaaaat
    14041 gtttgtttaa attatacttt tcacctattg tatagatttt ctttagagct tctttgctca
    14101 aaaccttctt atactcatat cccagtaatt taaaatatgt cccattagtt gatataatat
    14161 atatatcatc ccctaaaaca cttgtaaaat atttcatagc attataaggt cttcccgtaa
    14221 ctaaggctac cttgataccc ttttccatgg cttcttttag tgctttaata ttttcttctg
    14281 atacttcaaa tcctttaccc atcaaagtac catccatatc agtacaaatt aatttatatc
    14341 gcatttgtat cacatcctag taagattttt ctaatatttc aacaaggtct tctttctttc
    14401 caaaaggtaa aaatcctaaa actggacagc ttgttatttg ttctaattgt tcctttgtta
    14461 cattaaactc agacattttt gtcttaagcc ccactctctt taaaaaactt tctatttcat
    14521 ttcctaaagt tttagctgct tcatcatctt ccactttttc taattcttgg tttagtattc
    14581 tcgctacttt ggcaaatttc tctatattat ccttatagaa cttttctgca aattttggga
    14641 acactatagc caaagcctca ccatgaggaa tatgtgtaac tcctcctatt atttcactta
    14701 gtggatgtgg tgctgctgct ccagcatttg ccagtgaact tcctcctaat gtatctgcta
    14761 ctgctaactt tgttctgtat tctatattat ttttatcttc cattacttta ggtagatagt
    14821 taatgactaa ttccatagat ttttcagaca ttatctctgt aaaatcatta gcatttggat
    14881 ttatataact ttcaaatgca tgggtaaaag catcaaatgc tgttgatgca gtaattctct
    14941 ctggcagagt taacataagc tctgggtcaa ctatacattc ttttgagaaa caattttgat
    15001 gaaatattgt atttttctcg cttcctctcg atattactgc tgcttgtgta acttggcttc
    15061 ctgttccaga agtagttgat acagctatta acggtaattc tttttcactt accttatcgt
    15121 atattttaaa tggatttgta tatgtactaa acatataatc ccaatctatc ttttctaaac
    15181 caaaagtcaa tgctaatact tttgcagtat ctatacttga acctcctcca acagctaaaa
    15241 caacatctac agattctttt ttagctaatt caaacccttc atttattaat tctacagttg
    15301 ggtttggctc tactctatca aagtgtacaa cttctatacc tttttctatt aaattcaatg
    15361 taactctttt atataacttg tctaatggct ctacatttgg agtagtaact aacaaacatt
    15421 tctttccata ttttcctatt attctaggta attcacttaa agttccttgt ccaaaattta
    15481 ttcttgatgg ttgataaaag ttaatcatat ttttcctccc ttaaattatt gccttcatat
    15541 tcaaattata catatcatag gatttttaag gcaatacctt cactgttaac tcttatttac
    15601 taaagcttat ggcaattatt agacaatcaa tgttttatta ttaccatatc attatgtaaa
    15661 ttttattaag tacccatgtt ataattttat aatcaatctt attgtataat tgacttaata
    15721 ataaaaatag agtatataca cttttttatg acaaatagct taaaggagaa ttaaattatg
    15781 aaatatttga tagacagtgc taatcttgat gaaattcaaa agtgtttaaa gtatggattt
    15841 aagggaataa cagctaatcc atctatgtat cttaaagaga aagtaaactt ccatgaattt
    15901 attgagacat gtgctaattt aaatccagaa atattaactg ctgaagtcat tggaaataat
    15961 ttatacgaat taattaattc atgtgataag atattaaata taaataaaaa cactataata
    16021 aaaataaatt tctctgaagt tggcttagaa cttataaaca tattaaatag taaaggtgta
    16081 aaaactgcat gtaccttgat tttcaatgta aatcaagcat cattagctat aaatgcaggt
    16141 gctgattacc tattcccttt tataggtaga aatgatgaaa atgggaatga tggtataaaa
    16201 aaccttacag aaatatgtaa ccttatatca ataaatggat ataatactaa agttgttgca
    16261 gcttctatta aaaatatatt ccacttaact caagcatctc tttgtggatg tgattatgca
    16321 gctgtaacat atgatttatt taaaaaagct tcttttaacc agcttacaat agatggtgcc
    16381 aatacttttg aaagagattg gtccaaggtt aataaatagt aactaagatt taggaggttt
    16441 tgcattgaac aaaagattat tagatattgc tgaattatta ctcaacagct cagactatat
    16501 tactgtagat actattgccg aagaattaaa agttagtaat aaaactattc gtaatgacct
    16561 tgttatttta gatgaatggc ttttagaatt taatttatcc ctagataaaa aaactggttc
    16621 aggggttatt attctaggta atgaagatat aaaacttaaa gttattagag atatcaatga
    16681 taaatcaaat tgtatatatg catactctcc tgaagataga aaaaggtata ttctaagtaa
    16741 attatttatt aaaaattcta agtttaggat tcgtgatatt tgcaatgaac tacatgttag
    16801 tagagctact gtgcataaag acttggtagt tattcaaaat tttttgcaaa actttaaagt
    16861 gactctagtt agaaaaacca ataatggagt atatttagag ggaaaagaga aagatattcg
    16921 aaaagcaatt tttgaacttg ctactgttaa taaaagttat actgaactta aagaaatatt
    16981 attctcgaaa tctaatgaat gcaaagatac cccatcaaca aaaatattta aggagctatt
    17041 taactatgat tttgagaaat tatctcaaat cactctacac accttacaag tagaaaaaag
    17101 taaccttagt gatgagtatt atataaactt cttaatacat atagctatat gtattaaaag
    17161 aatttcaatt aaaagatata ttaacttatc tgaaggtttt tttagagaat tattagaaca
    17221 tgaatacttt gaaaaatcaa aaattttatg tgctaaccta agtgaagctt ttgacgtaga
    17281 atttattgat gaagaggtct gttatatcct tcttcatata gaaggattac ttaaatcaat
    17341 aaagaaaact actactcttg aacctcttaa aaatgaagaa attacagagt ataatctatc
    17401 tgaagccata gctgttcatt ggggaagtat actaaattta aacttaaagg atgatttaat
    17461 attaataaag tctcttgcta atcaccttaa atcagttcta cacagaataa actatgggtt
    17521 tactattaca aatcccatat taaatgatat aaagagaaca tttccttaca cttataaggc
    17581 tgctaaagag tctaatactg ttatcaaaca gttgatgaat tatgaagtca atgatgacga
    17641 aataggatat cttgctttat atttaatatc agcaatagat agaagcaaat ctcccctaaa
    17701 tactatttta atatgtcact gtagtgatgg tgtatctaat ttattagtcc aaaagttaag
    17761 ttttgaattt aatcagatta atatagttaa atctatacct ctatcttcta tttcatttac
    17821 gaactttgat gatgtggact taatactaac cactgctcct gtagactttg aacataatgc
    17881 tgaactgata aatataaatg ctcttttaag taagaatgat atttctagac ttagtacaat
    17941 tataaaaaaa ctatattctg aaaaaaataa aatattatct tataaataaa caaaaatagg
    18001 cttgtctcaa catagagata aacctatttt tcattgtcca aatgataaaa atatatttga
    18061 tttcatagaa aaagtgcctc atgaactaaa aaattcattt tgagacacct tcatttttta
    18121 tatttaatct ttttatttaa gcttttatat tttttaaact tttatattta aagtaaattt
    18181 tattattttt attgagcaca agcattattt atattattaa tttcttcagt attcttacca
    18241 aactttgtag ttacaaatcc tattgttttt attatgagta ataaggatat caatgaatat
    18301 atagatatca taaaattctt acttattatt ccagacgcta ctattataaa tccatctagc
    18361 ataaataata taactggcaa cttcagaaac ttaattacct tattaataag cattgcaact
    18421 aaatcagttc caccagtact acagcccctt attattgcta tccctatagc agttcctacc
    18481 aataacccac ctatcactcc tgctactaat agattgtcag ttatgtgagt ttgtggtatt
    18541 acttttaaga ataatgggcc cataagagtt atatataatg tttttattcc aaaacctttc
    18601 cctaaaaata ataatcccat tactaaaagt ggcatagata ttcctaatga tatattctca
    18661 acaggcattg ataagaatgt gctaagtacg actgataatc ctgttatccc tcctggagct
    18721 aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca ttaaaaatgc tgatattgtt
    18781 atcagtaaat aatcttttat catttctttt ttcatttaat aacctcctat gattatattg
    18841 tttcagtata aaaaatcaat taaattagtc tagcttcctg tagtttttca taaaattaat
    18901 gcttccttaa aacattaaga aagcatctct tttatcatag tttattatct tattctaaac
    18961 caaactttgt agctgcttca tctatagctt ttctacaatt cttaacacct gctgttatac
    19021 catctttatc cttgaatatt gctgaagata atattacaac gtctggatta cattctatta
    19081 atggctccat attgtctgct cttagtccac catctattgc taattcacac ttaggattct
    19141 tttcgtctat catttttcta gctcttttta ctaaatcaac agcactcttt ctccatcccc
    19201 agttatcttt tccatcagtt tcatcaacac catgaacgac tatttgcaat ctatctatat
    19261 catatatagc ttcatcaaca aaacataatg gtgtgtaaca tccaattgtt aatcctactt
    19321 tcataccaaa ttctctacag taatttatta tgtatgcaag aggagcacct aaaaaatgtt
    19381 ctgctggtat tatcaacaag ttacatcctg ctgctgctat tttttctata aataatctat
    19441 cacaatctct tgtgtatata tggcattcta ttggtttatc tgtcaccggt cttattgctt
    19501 ctattatttg atgaccaccc ataagttgca tattttttaa gtcatgcata tctgctgcat
    19561 ctgaatgtac gtagtctaca cctgcatcca ttgcttcttt aactacggct gatatatgac
    19621 cgtaatttac atgtgctaat cctgctgcta tttttatgtg tttcatattt gtatacctcc
    19681 atattttact ttaattagta ttaaatatta gtttttaata tttttttaat attttttaat
    19741 ccagtattac ttttatttag cattactttt attgctgtat atatttcttt tatgaactta
    19801 ttctgctttt gttaactgtg aacttatttt gctttgtaaa ttaatattac actgatatat
    19861 tttttttcac aacactttga ctactcttta aaagtacctt tatattgtgg taaattatag
    19921 acattaattg tatataaaaa gttagttttt taccacattc ttatggcatt tattgtctat
    19981 actaactcaa ttgatatagc tagttcattt aatatataat cagactataa atataaattt
    20041 actcgataaa aagtaccatt cataacaata ttaatgaaag ggagatgtaa aatgagaaac
    20101 tgtattattg cccaatctgg aggccctaca gctgttatca atgcttctgc agttggtatt
    20161 tatgaaaaaa atttagaaac taaatacttt gataaagtat attttggtat caatgggatt
    20221 gaaggtatat taaacaaaaa tataatagat tcagactcct taaacaaaga taatgtatta
    20281 aatttaaagc aaacaccttc gtctggtctt ggttcttgta gatacaaact ggctgattat
    20341 aaaataaatt cttatgatta tgatagagtt ataaatatac tagatgaata tgaaatagat
    20401 acattatttt atataggcgg aaatgattct atggacacag tgatgaaatt ttctcaatac
    20461 tgcaaaaaca ctacttgtaa aataaaattt ataggtattc ctaaaacaat agataatgat
    20521 ttattaggaa ctgacaactg tcctggattt ggttctgctg caaagcttat ttctacacta
    20581 ataatggaaa catacttaga tttttctata tacaaaaata atggtatatt tatagtagaa
    20641 actatgggta gagatactgg atggttagca gcttcttctt cactagctag acttaatgat
    20701 actccacttg ttgatttaat ataccttcca gaaattgatt tctatgaaga taagtttata
    20761 gaagatgtta gaaaaatatt taaagaaaaa aataaggctt atatagttgc gtcagaaggt
    20821 ataagggata aatacggcaa ctttatatgc gaacaaaaaa cttctgctca tgataaattt
    20881 ggtcattccc aattaggtgg agtaagcaac tatctaaaga atcttatagt atcatcatca
    20941 attacaacaa gagtaaaaac cttagaactt ggtgtaatac aaagatgtgc tatgcattta
    21001 gcttcagata ctgatttaaa acaagcagct gaagttggtg catatgggat agaactatct
    21061 aaaaaaggat atacaggagt tatggctatc attaatagaa catgcaataa tccttataag
    21121 tatgaagtta gtcatactga tatttctaaa atagctaaca agacaaaata ttttcctaaa
    21181 gagtggataa atgaagaatg taattatata actgatgaag ccctaaatta tctatatcca
    21241 cttattcaag gtaaaccaga tatcaaatat aaaaatggac taccttattt ttcaattatt
    21301 tactaggaaa ttattataat ttgatataca ctcaattcta tcgctcttaa atacctaagt
    21361 ggctgttaaa taaaattata aattgttata tgacagtcac ttaatcgttt aattatttaa
    21421 ctgtttagtt ttttaatttt ttaatctttt taatactaat acaatttgtt gtaataatat
    21481 ctcacaccca ttttcatttt ggatagatta taattagtta gacaataaat attttgtcat
    21541 gatattatta tatatagtga tacaaataga aatactttta tcatagctag aaaaacaaag
    21601 attacacaaa ggatttaaaa atcaaacaac ttcaattaaa ataaatggtt aaaataactt
    21661 ttaaatcaac tacataaaca ataaaagaac ataaagtagt taagtctaca attataaaat
    21721 tagtaagact ataataactt aaataaaatt aaagttaaca aagactatta aagttagcaa
    21781 atattcctct ac
//
LOCUS       DAELJO010000051        21569 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELJO010000051 DAELJO010000000
VERSION     DAELJO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.051"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            279..983
                     /locus_tag="KPB88_001616"
     CDS             279..983
                     /locus_tag="KPB88_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0053898.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            985..2472
                     /locus_tag="KPB88_001617"
     CDS             985..2472
                     /locus_tag="KPB88_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0053899.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTGNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2677..4290
                     /locus_tag="KPB88_001618"
     CDS             2677..4290
                     /locus_tag="KPB88_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0053900.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4653..5483)
                     /locus_tag="KPB88_001619"
     CDS             complement(4653..5483)
                     /locus_tag="KPB88_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0053901.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIRPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5556..7283
                     /locus_tag="KPB88_001620"
     CDS             5556..7283
                     /locus_tag="KPB88_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053902.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7320..9182
                     /locus_tag="KPB88_001621"
     CDS             7320..9182
                     /locus_tag="KPB88_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053903.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9515..10204
                     /locus_tag="KPB88_001622"
     CDS             9515..10204
                     /locus_tag="KPB88_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG0053904.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10309..10995
                     /locus_tag="KPB88_001623"
     CDS             10309..10995
                     /locus_tag="KPB88_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG0053905.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11012..12919
                     /locus_tag="KPB88_001624"
     CDS             11012..12919
                     /locus_tag="KPB88_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG0053906.1"
                     /translation="MKDKKFTLLISIMTVFLCAIVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13372..14061
                     /locus_tag="KPB88_001625"
     CDS             13372..14061
                     /locus_tag="KPB88_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053907.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14497..15036
                     /locus_tag="KPB88_001626"
     CDS             14497..15036
                     /locus_tag="KPB88_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0053908.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15055..16098
                     /locus_tag="KPB88_001627"
     CDS             15055..16098
                     /locus_tag="KPB88_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053909.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16100..16936
                     /locus_tag="KPB88_001628"
     CDS             16100..16936
                     /locus_tag="KPB88_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053910.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17041..17715
                     /locus_tag="KPB88_001629"
     CDS             17041..17715
                     /locus_tag="KPB88_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053911.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17715..18767
                     /locus_tag="KPB88_001630"
     CDS             17715..18767
                     /locus_tag="KPB88_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0053912.1"
                     /translation="MIKFKKVISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            18997..19845
                     /locus_tag="KPB88_001631"
     CDS             18997..19845
                     /locus_tag="KPB88_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0053913.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19835..21499
                     /locus_tag="KPB88_001632"
     CDS             19835..21499
                     /locus_tag="KPB88_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0053914.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
BASE COUNT         8462 a         2157 c         3735 g         7215 t
ORIGIN      
        1 actacacctt caaatatcac ctaaagtaat taaaaggcaa ttaaagtgaa gtagttcaag
       61 tatttctaaa ataagtttaa taaattctat aataatttat tttttgcctt aaaattgcct
      121 taaaatccaa acaattttaa aactaatgta ctataaaaag ctggtataat ttaaatatag
      181 taaaaaataa gatttatctt atctattggg atttaattat ttaatcaata tatttatacc
      241 atttaattta aataaaaagt ctattaagga gtgagcttat gagtaagtta atttatatag
      301 cagatgatga agataatata agaaatttgg taaagacatt cttaaaaaat gaaggtcatg
      361 atgttatgga ttttaaaacg ggtgatgaac ttttagaaca atttaatata aaggagtgtg
      421 acttagtaat actagatata atgatgcctg gatcaagtgg ttttgaagta tgtacaaaac
      481 ttcgtgagaa aagtacagtc ccaataatca tgcttactgc aagagataca gatatagatt
      541 atattacagg gataacctta ggtagtgatg attattttac aaaaccattt agcccaatgt
      601 ctttggttat gagagtaaaa tctatattta gaaggataga gtttgaaaag aaacaaaatt
      661 atgataaata tagtaactcg atagatatgg aattaaaatt tggtgatgta ataataaata
      721 aaaagaataa aatcgttaca agtaaaaatg taaatataga ccttacacca aatgaatata
      781 acctattgac ttatttgttt gaaaatatag atagagcagt ttccagagat gaattattaa
      841 acaaaatatg gggatacgat atagaagttg agacaagggc tgctgatgat acagtgaaga
      901 gattacgtaa aaaaatttta aatacaaata ttttaataga aactgtatgg gggtttggat
      961 ttaggttgaa ggagaaaagt taagatgaag aatggaaatg acaagaatag aattggaaag
     1021 attaaatcca aatttaaact aaagttctca aagaaaaata taagcataaa aactaggatt
     1081 ttgttgagta tactttgttt gataattata gtatttatga taatattagt atcttttaat
     1141 gtatttcttg atacatatat aaaaacaact gcgaatgaag aattgacaaa gtctacagaa
     1201 atagtagaac atatggatag taattttaga cctattaagc caccacaaga tgatggaaag
     1261 cttcctccca aagtattatc taattttatg agaaatgtac aagataaagt taggatggca
     1321 gaaacacaat cagatgctga tgctatggta gttgattcta agtataacct gatatttcca
     1381 acaagagagg atgatttctt aaaaaatgtt gatgagatgg agctgattag ggagtgtatt
     1441 caaaatgaaa aattagattt aaattcagat gcaaatacac ggatatctac cggaaataag
     1501 gattattata tttcaactgt aaaaattact agtatatatg gtgaacaaga caagtatctt
     1561 atacttttta tagacatatc aaaaactcta aatctagcac aaaagataaa tattgtatta
     1621 atatctgtaa tgtgttttgc tggaatatta gcaatattta cagctgtgat tttatctgag
     1681 aaaattgcta aaccgataaa agagttgtgt gagtttgcaa aaaaaatggg tcaaggagat
     1741 ttcaaaagaa cctactttga cttttcagat aaagaattag tagagctctc aactgttatg
     1801 aataagagtg ctgagtatct ggataagtat gataatgaac aaaaaatatt ttttcaaaat
     1861 gcatctcatg aattaaggac accacttatg tctattaaag gttatgcaga agctataaaa
     1921 tataatgtta tagatagtaa acatgcaagt gatataattc ttgaagaaag tgataggtta
     1981 agtgatatgg ttgaagattt attatacatt tctaaaatag ataatataac taaagatttt
     2041 gaacttgtag aatgtgattt aagagaagta ctttcaaatt gtggagctag gcaaaatgta
     2101 agagctataa ataaggggat aaaatttatt tatgaatttg atgaagaaat ggttttattt
     2161 gaatgtgatg aaaaaaacat ttccaaagca tttatgaatc ttatagaaaa tgctttgaga
     2221 tacgctaaga gtgaaataaa aatagtgtgt aagtataatc aaaaaaatat agttgtgata
     2281 atagaagatg atggaattgg aatcaagaaa gaagatttac ctcatgtttt tgaaagattc
     2341 tataagggag taggaggaaa tcatggaatt ggattatcaa tagttaaatc tattgtaaat
     2401 aagcatggtg ggcgtatata cgtagaaaat ggcaaaaaag gagctaaatt tactatatta
     2461 tttaaattgt aaaaaaatta gagaaaatat agagatgttt gaagttgttt tttatatata
     2521 tctgttgtat actgttgaaa aaagaacaat attaagctga catatattac gatagttgac
     2581 atttaaagta aatgcttaaa tatagatacg aaaggaaaag taaaatctga agtgtattta
     2641 ggtttaagta agcttcttat tttataaaaa atatgtatga aagaacagct gatttttaat
     2701 attacaaatt tagtaacggt tgtatttgaa gcttttgtca tccatatgtt tttatcagac
     2761 tttttaggtc agaaagaagg gtatataaaa gttgtaagat atgctaaact gggttttata
     2821 atatgtttag gattctgtaa tttaataact ttaaacccta aaatcacaat gcctttaatt
     2881 ttcatcctta ttttttcaac atcattttta tataaaggaa atttaaagac acgattgttt
     2941 actactgttt tattaagtat attctttata ctgtcagaaa ttgtagttac aagtattttt
     3001 gttttatttg taaaagaagg ctttgaaata atgcttgaaa ataatagtat aagagtattg
     3061 gcgacaattt tatctaaaat tgtctttttg ttgacttgta aaattatttg tctatttaaa
     3121 aaggatgtac atttagatat gcccataaag tattggctcc ctttattttt aatacctata
     3181 tttagtttat ttctgtctgt ttctatcttt gatgtaagta aattttttag tcttgaaagt
     3241 ttaaaatttc taagcttaat atctagtgtt ggtattttat atattaattt tattgtcttt
     3301 tatttattta aatttattat agataaaact aaactatcta tgaaatatga actacttgaa
     3361 aaagaaataa ttcataagga agaattaaga cttagtaatg agtgttataa aattattgtt
     3421 gaacagactg attctgtcgt tttcgaatgg aatatcaaag aaaataaatc atttgtatcg
     3481 caggcttgga ctgaaaaatt tggatataac aatgcatgta agaatatttt taaagaaatt
     3541 aaagataaag acttagttca ttctgaagat aaagctatat ttgaaggttt tcttgaaagt
     3601 attaaaaaaa agaatatgca caatcaagca gtatatcgtt taaaaaaatc taatggtgaa
     3661 tatatttggt gtagaacatc aattacaagt atttataatg atgaaaatga acttttaaga
     3721 gttgtaggag taattgtaga tgtagatagt gatataaaaa aatatgaaga gttaagaact
     3781 agagcagaaa gtgactcatt gaccaatata tacaataaag gtacatttga aaaattagtg
     3841 gaagaaacta tagttatgaa tacaggtgat aaaaaagatg cattattcat tattgatttg
     3901 gatgatttta aagaaataaa tgataatttt ggtcatccat ttggtgattt tgtactaaaa
     3961 acatttgctg ataagataca aactagtttt ggtagtaaag acttagtcgg aagaattggt
     4021 ggagatgaat ttgttgtata tatgcaagat tatgttacag aagtaaattt gcataaaaaa
     4081 gctaaagaat taaatagagt actttcggat aattacactg atttgagttt tagttttgat
     4141 gcttctgtca gtataggtat tgcaaggtat cctcaagatg gaacttcatt ttttgaattg
     4201 tttaaaaatg cagatagagc actttatagc ataaaagcat caggtaaaaa ttcatattgt
     4261 ttatttgaag aagaactata tgtacagtga atttgaattt agattttatg tgagagtaag
     4321 atttatatat acttgtggct gtctgattta taaaatatgt tactttgtat atttttatat
     4381 gtttcagtaa ggttaattaa cataagcaaa ggtgtaagta aatttatatt agtttaagtt
     4441 tacttacacc tttataaaaa tacaaataat agattcattt acattcttaa atgattaaag
     4501 ctctttacct tctaaacatg cataacatat cgttataatt ccagtaaata agactaaaat
     4561 gcccattcct aagattacag tttcatcatc tattactcca aatgttttta aagtaactta
     4621 tttcgctttg tatcataata atttatctcc ttttacattt taacttctgc tgagatttga
     4681 gttagatatt cgttagaatc tcgcacactt atatcatata gaagatattc ttcataaaca
     4741 aaatccccaa tacgtaaatt attctgatta aaaaaattta ataacttttc ataagtcttg
     4801 taaatagttt cataactccc tttatgataa gcagttacat aaagtccctt tggtctgatt
     4861 gatacaggat atttcttgta ctctttatta acttttgtaa ataaatatga atatgattca
     4921 aagtctttat taattatatc ttcaccttta atgattgagc caatcgtacc ataatcactc
     4981 aattcatagt tgtccataaa gttaatacat tcatataaaa aattttcttc ctcatctcct
     5041 aaaaattgtt ctttatatgt aacaggtgtt ttgaccaagt actcttcttc atgttctttt
     5101 aaagtaatag tattcgcatc tatattacag gcatttttag tgaatgaaat agtagattcc
     5161 attagagctt gaatattatt tagtttttca atttcatttt taatatcaat ggttttcttt
     5221 tctaaaagct caattagtaa attaggtgtt cttttgtcta tataagcttt aatttctttt
     5281 aaaggcattt ttacttccct aagtgaagaa attataccaa aaatgctaag ttgatggtat
     5341 gagtagtatc tatatccatt ttctttcttt aattctggtg agaaaagacc tatatcatca
     5401 taatggaaaa gtgttttttt attgattcca caaatctttg caaattctcc agttgtaaaa
     5461 taattttttg aaacttgact cataattata attcctcctt gactatcctg ttacagtata
     5521 gtttatcata gtattttgta aaggagtgtg attatatgaa aaaattaatt cattttttaa
     5581 aaccatatag agttttaatt gttgtagttt tgatatttac gtttttacaa acacttggaa
     5641 ctttgtatat accaacactt acagcaaata tagtaaataa tggtgtagta aagggagata
     5701 tagattatat tgtaaaaaca ggtttaatga tgatgatagt agctggtata acagctttat
     5761 cagctgtgtt agtttgtaaa gtatcagcaa atctttcatc tggattttgt agagatatta
     5821 gagaagcagt atttataaaa tcacaagatt tatcaataaa tgattttaat aatataggta
     5881 cagcatcaat gattactagg agtactagtg atattacttt aataggtcag tctgtgttta
     5941 tgtttattca attagtatta ccagcaccaa ttataactgt ttctggtcta ttcttagcat
     6001 attctataga taaagcaatg acaattatta ttgttgtagt aatgttttta ttcatgcttt
     6061 cagcattttt agttggtaaa aagcttataa agttgtttaa aatgatgcaa ataaagatgg
     6121 acaatatgaa tagggtgtta cgtgaggtag taactggagt aagagttata agagctttta
     6181 ataggagtcg ttttgaaaaa aagagatttg atagaactgc aatagattat tctgaaacag
     6241 caatttcaat aaataaaata tttgcagtac ttatgcctat tgtaatgtta ataatgaatt
     6301 taggtatagt aagtattatt tggtttggtg ggatgagagt cagtaatgga aatatggaaa
     6361 taggtcatat aatggcactt gttgaatatt gtatcttaat tttattctat ttaatcatgg
     6421 gagtaatggt atttatgtac atacctagag caggtgcatg tgcagataga gtaaatcaga
     6481 tattagatat agaacctgaa attgtagatg gaaatggtca taaagatact gtctcagaga
     6541 gaggacattt agtatttaaa aatgtaacct tttcatatgc acaatcagaa gaacctgtgt
     6601 taaataatat aacatttgaa gcaaaatctg gagaagtaac tgcaattata ggaagcactg
     6661 gctcaggcaa atctactatt gcaaatataa taccacgttt ttttgaaatt caatctggag
     6721 aaatatctat aaatggtcag gatattaaga agattcctca aaaagaactt agagataaga
     6781 ttggatttgt accacaaaaa gcttttttat ttagtggaac aatagaagaa aatataaggt
     6841 atggaaaaga agatgcaagt attgaagaag taaagcatgc tgcaagtata gctcaagctg
     6901 atgaatttat ttctgatatg gaagacaagt atgactcatt tgtagcacaa ggtggaaata
     6961 atttgtcagg tggtcaaaaa cagagaatat ccattgcaag agcgttagtt agaaagcctg
     7021 aagtatatgt ctttgatgat agtttttctg cattagattt taaaacagat aaaagactta
     7081 gaaaagcgtt gaaaaatgaa atcaaagatt catcagctat aattatagca caacgtataa
     7141 gtacaatcat ggatgctaat cagattatag ttttaaatga tggaaaaatt gtaggtattg
     7201 gaaaacataa agatttactt gaaaattgtg aagtttataa gcaaattgcg gattcacaac
     7261 ttagtaaaga agaacttgca taagatgaag aattaatctg atatttggag gtgtatgata
     7321 tgagtgaaaa aagaatagat gaacaagata tagaagtact tagttttgaa gatggcatgt
     7381 cagcagaaaa agctaaggat gcaaaaaaaa ctactaagag attattaaaa tatatggcaa
     7441 aacaaaagtt taaaatatta attattttta tttcagtatt gataagttca gcgcttacag
     7501 ttttagcacc tatggttatg ggaaaagcta ttgaccaact atttaatgga ataaaaactg
     7561 cagtccaaac aggtacaaaa ttcagtgtga attttagtac tatgggaggg atagtatcaa
     7621 tacttctagg gttgtactta attagtgcag tatttatata tattcaacag tatattatgt
     7681 caggagttgc acaaaatcta gttcttagca tgagaaaaga tttaagtgat aaactcaata
     7741 aacttcctct taagtactat gattctcata aaaaggggga aacacttagt atagtaacaa
     7801 atgatttaga aaaagtagca gatagtttac aagaaggatt aatgcaactt attacagcag
     7861 tagttacagt agttggttct attgtaatga tgataagtat aagtgtacca cttacaatag
     7921 tatcagctat aacattactt gtttcattgg gtattactgt tgtaattgca agaaaatcac
     7981 aagaaagatt ctctgaaaat caaaaggcac taggagagtt aaatagcaat atagaagaga
     8041 tatttactgg tcaaatagtg gttaaagcat ttagtaaaga aaaggatact ataagaaact
     8101 ttaaaatatt aaatcaaaat ctttataatg caagtagaaa agcacagttt tcgtcatatg
     8161 caatatctcc aattattaga tttataaacc taattggcta tgttattata gcagttgtag
     8221 gtgggatatt tgcatcaact ggagcaatga ctcttggtag tatacaagcg tttattcaat
     8281 atgtaaatca agcatcagag cctactacag aaatatctta tattgtaaat atgcttcaag
     8341 cagcaatagc atctgcagag agagttttta cagttatgga cgaggtggaa gagataaagg
     8401 atgaagaatc ttcaaaggtt attagcatgc ctaaaggtaa agtacaattt gagcatgtta
     8461 aatttggata tagtgatgat tttattctta tgaaagatat taatatcaat ctaaatgcag
     8521 gagataagat tgcaattgtt ggacctacag gagctggaaa gacaactctt gtaaatttac
     8581 ttatgagatt ttatgagata caaggtggaa gaattactat tgatggtgtt aatataaaag
     8641 atttaaagcg tggagaactt agaactatgt ttggtatggt tcttcaagat acatggctgt
     8701 ttaatggaag tataaaagaa aatattgcat atagtaagtc agatgcaaca atggatgaaa
     8761 ttgtaagagc agcaaaatca gcaagagtag accattttat aaggacttta ccacaagggt
     8821 atgacactat attgactgag gatgcttcta atctatctca aggtcaaaag caacttctaa
     8881 caattgccag ggcaatactt tcagacccat cagtattgat tcttgatgaa gcaacatcta
     8941 gtgtagatac tagaacagag gtagaaattc aaaaggcaat gaataacttg atgaagggaa
     9001 gaactagttt tgttattgca catagattat caactattcg agatgctgat ttaatattag
     9061 taatgaaaga aggaactatt attgaacaag gaagccataa ggaacttatt gaaaagaaag
     9121 gattctatga agaactttat aacagtcaat ttacaagtga atatgatgaa gatgttgtat
     9181 gattatcaag ttttaggcaa ctaaatgatt gataaatatt aagttaaata tagtaacatg
     9241 taaggttata tggaataaga gtatttctct atttcatata acctttttta tttatcaaaa
     9301 taagataaaa tttcctttct aaattagatt gaagaatgta tttatcaata taaaagtaaa
     9361 ataatcatta ttattagtga agtaaatatt gtttgccaca tatatgccat aaagatgcac
     9421 ttattttacg gagatttaac aaagtagtgt aaagtatact ctaaatataa aataaatcaa
     9481 atagagttaa ataaattgag aggtgatata aatagtgaat aaaaaattga actatagatt
     9541 tgaaatgaag cacaaaataa ctgaagcaga tgtgttagct ttaaaatcaa gactctatcc
     9601 aataatgaag aaagatgaaa atgcatctaa agatggaaaa tatcttatta ggagtttata
     9661 ttttgatact cctgaagata aagcactttt ggataaatta aatggcgttg caataagaga
     9721 aaagtttagg ataagatttt ataataatga ctgctcttat ataaggttag agaaaaaaat
     9781 caaacattac aacatgacat caaagctatc aactaattta accaaaaaag aagtaataaa
     9841 tatactaaat aatgatattg aatttctaaa agagtcgaca aattgtttat taagagaatt
     9901 ttatttgaaa ttaaaatgtg aaagattaga ggcaaaaagc atagttgatt acaatagaga
     9961 agcttttatt tatccagttg gaaatgtaag agttacaatc gatagtgata ttaaaacttc
    10021 tgtaaattcg gtagatttat ttaataaaga tttgccaact gtttcggtaa ttgatgagaa
    10081 tatgactgta ttggaggtta aatatgatga atttatacca gattttataa aggatttaat
    10141 tcaaattaat aagactacct ctacagcagt ttcaaagtat gcttcaagta gattatatat
    10201 ttaagaataa aggctatgat ttatataaag atatacataa ataaggttaa aaaacaaata
    10261 agttaaaaat aaataaaata aaaaataaat aagttaaaaa taggaaggat gattttaatt
    10321 atgacattta atgatatttt taaatcaagt tttatagaaa acgtatcagg tttttcattt
    10381 gtggacagtg ctttagcact aggttcagct tttttagtag gtttgtttat atatatggtc
    10441 tataaaaaga cttatatggg aattatgtat tctcgaccat tcaatgtgtc gcttgtagca
    10501 cttacaatgc ttacaacttt tgtaatattg gcagttactt caaatgtagt tttatcctta
    10561 ggtatggttg gtgctttatc tatagtaaga tttagaacag caattaaaga cccaatggat
    10621 ttagtatttc tattttggtc attgggttca ggtatagttt taggagcagg tttgattccc
    10681 ttagctgtca tgggttcaat aattatggga ttgattctaa tattcttttc aaataaaaca
    10741 atatctgaaa ctccatatat attgatggta aattgtaata atgaagattc agaagatata
    10801 gcaactgata aaattaaaaa agtatttact aaatatcaaa taaaatcaaa aagtgttaca
    10861 ccagaaaaag gaatagaatt agtgtttgaa gtaagaatga aagatggaga aacaagtctt
    10921 ataaatgact tgagtcaagt agatggagtt actaatgcag ttcttgtaag ttataatggt
    10981 gactatgtag catagagagg agtaatcttt tatgaaagat aaaaaattta cccttcttat
    11041 ctcgattatg actgtatttt tatgtgctat agttggagtt tatagtacat ctagcaacaa
    11101 aagtgttgat ttatatagtg atgtatatat tgaaaaatat tttaacagag acaaggttat
    11161 ggaagttaat atagagatag atgaaagtga cttgaaggat atgaatgaaa atgctataaa
    11221 agaagaattt aaggttgcaa aagtaactgt agatggagat acatatggaa acgtaggtat
    11281 aagaactaaa ggaaattcaa gtcttatatc tgtagcaaat agtgatagtg atagatacag
    11341 ctataagatt aattttgata agtataatac tagtcaaagt atggaagggc ttactcaatt
    11401 aaatcttaat aactgttact ctgacccatc ttatatgaga gagtttttaa catatagtat
    11461 ttgcgaggaa atgggattag cgactccaga atttgcatat gctaaagtct ctataaatgg
    11521 cgaatatcat ggtttgtatt tggcagtaga aggattaaaa gagtcttatc ttgaaaataa
    11581 ttttggtaat gtaactggag acttatataa gtcagatgaa ggaagctcgt tgcaatataa
    11641 aggagatgac ccagaaagtt actcaaactt aatcgttgaa agtgataaaa agacagctga
    11701 ttggtctaaa attacaaaac tattaaaatc tttggataca ggtgaagata ttgaaaaata
    11761 tcttgatgta gattctgtcc ttaaaaatat agcaataaat acagctttat taaaccttga
    11821 tagctatcaa gggagttttg cccataacta ttatttatat gagcaagatg gagtattttc
    11881 tatgttacca tgggatttta atatgtcatt tggtggattt agtggttttg gtggaggtag
    11941 tcaatctata gcaattgatg aacctacgac aggtaattta gaagacagac ctctcatatc
    12001 ctcgttatta aaaaatgaga catacaaaac aaaataccat aaatatctgg aagagatagt
    12061 aacaaaatac ctagattcag actatttaga gaatatgaca acaaaattac atgacatgat
    12121 agcatcatat gtaaaagaag acccaacagc attttatact tatgaagaat ttgaaaaaaa
    12181 tataacatct tcaattgaag attctagtga taataaggga tttggtaata aagggtttga
    12241 caacaataac tctaataaca gtgattctaa taataattct aatagtgaaa ataagcgctc
    12301 tggaaatcaa agtgatgaaa aagaagttaa tgctgaatta acatcaagcg tagtcaaagc
    12361 taatacagat aatgaaacta aaaataaaac tacaaatgat agtgaaagta agaataatac
    12421 agataaagat aaaagtggaa atgataataa tcaaaagcta gaaggtccta tgggtaaagg
    12481 aggtaagtca ataccagggg ttttggaagt tgcagaagat atgagtaaaa ctataaaatc
    12541 tcaattaagt ggagaaactt cttcgacaaa gcaaaactct ggtgatgaaa gttcaagtgg
    12601 aattaaaggt agtgaaaagt ttgatgagga tatgagtggt atgccagaac cacctgaggg
    12661 aatggatggt aaaatgccac caggaatggg taatatggat aagggagata tgaatggtaa
    12721 aaatggcaat atgaatatgg atagaaatca agataatcca agagaagctg gaggttttgg
    12781 caatagagga ggaggctctg tgagtaaaac aacaacatac ttcaaattaa ttttaggtgg
    12841 agcttcaatg ataataatgt cgattatgtt agttggtgta tcaagggtaa agagaagaag
    12901 atttataaag tcaaaataag attaaaattg aatataaata attttaatac tgttaagtct
    12961 aaatatttac ttagcagtat ttttttagaa aataatgtat ttggttaata atataaggta
    13021 ataaattgat aaaaattaaa tacagcatat taataataca taataaagta ataaaaaaac
    13081 tgtttaagta taataactta tatttaggca gtttttttca tttataaata atataaatga
    13141 aattgaaaac atttgaagga tagaaattgg ttggataaat gtataaatta cttcaccaaa
    13201 aaataatgac aaaaattgta actaagttgt aataaaatgg tagttattta gcataaaata
    13261 atgtatcata gaagtatatt ataaaattaa taaaataaga tttttgtaag aaaactgctg
    13321 gcttttgata tttagagtga gtagttaaat tatgtttagg aggggtagaa tttgttgcaa
    13381 aaagtattat ggaaattttt aaagctagta gtcggtttat ttatatgttc agttggtata
    13441 gtattgacga taaattgtaa ccttggatta tctccctggg atgtatttca tcaaggactt
    13501 tcaaatcata taggaataac tataggaact gcttctataa ttgtaggctc tatagtagtt
    13561 attgctgatg tcgttcttgg agaaaatgta ggatggggaa ctgtttttaa catgctacta
    13621 attggatttt ttatggattt acttttatat agtaatttaa ttccagaggc tgatagttta
    13681 tttgtaggaa taattatgtt gatattagga ttggttcttt tgtctgttgg aatggtattt
    13741 tatatgggaa gtggtttagg aagtggacct agagatggtt tgatggttgc aattcaaaag
    13801 aaaacaggaa aatcacttaa attaataaga ggtactatag aagttggagc attaatagta
    13861 ggatttttat taggtggaaa agttggtata ggaactataa taagtgcatt tggattgggg
    13921 tatttcacac aaatggtatt tggcttattt aaattagatt gtagtaagat taaacataga
    13981 tttatagtag atgatataaa atttatcaaa gtgtatatga gtggtgagaa aaaagctgct
    14041 gttcaaaata tagttaagta atttaagaat aaattcaacg aaatgttaag tattttaaat
    14101 taacattttg tttagataga taaattgaat attaaaagaa tattaaaata tgaggattgc
    14161 tagaacacta taaaatatag atttctagca attttttatt tgttcaatat ttttttagta
    14221 ctagaagtct tattctctat ttaatgaatg aattaattta taattagaga tactaaactt
    14281 tatagatatt aatataaaca cctttaaact gtgtattttc caaagccttt aaaatgcttg
    14341 tatttattct aaataaaaaa ataaaaaaat gtcaaaaaaa ctcttgtatt atatccgaat
    14401 tttatatata attacctatg ttgtttagaa attttaaact caatgtttag taaacaaaat
    14461 gtttagtaaa ataaaacaga aactaggagg tgcaacatgg atataggtga aaagataaaa
    14521 agattaagaa cagaaaaaca actgacccag gaagagttag ctaatagatg tgaattgtca
    14581 aagggattta tttctcaatt agaaaataac ttaacatcac catccatagc tacccttata
    14641 gatatacttg aaatactagg tacaaattta agggaatttt ttaatgaaat agatgatgaa
    14701 agaatcagtt ttacaaaaga agatatgttt gaaactgaag atgaagattt aaaatataaa
    14761 ttaaagtggt taattccaaa ttcacaaaaa aatgaaatgg agccaataat aataacattg
    14821 tatccaggag gacagtataa agaagaaaag ccacatgaag gtgaagaatt tggatatgta
    14881 ttagcaggtt caatatatgt ccatataggc gaaaagaaaa ataaagtaaa aaaaggagag
    14941 agtttttact ttagaccgaa agcaaatcac tatatatcaa acgagggcaa aactacagca
    15001 aaagtgattt gggtaagtac gccaccatca ttttagaata gaggtgttta ataattggta
    15061 gaaaatataa tagaattaag ggatatatct aaacattatg aagaacttac tgttttagat
    15121 aatttcaatt tagatataaa gaaaaatgag tttctaacat tacttggacc aagtggatgt
    15181 ggaaaaacta caacattaaa aataatagca ggatttgagt atgctgatga tggtaaagtt
    15241 ttatttgaag gtaaggagat aaataatttg ccaccatatg aaagacaagt aaacacagta
    15301 tttcaaaaat atgctttatt tccacacatg gatgtttatg aaaatgtagc ttttggatta
    15361 aaaattaaaa agactccaaa agatgtaata gatgctaaag taaaagaggt tttgaaactt
    15421 gttgcactag aaggttttga aagacgacaa gttgaatcat tgagtggagg acaacaacaa
    15481 agaattgcaa tagcaagagc tctagttaat gaaccaaagg tattattact agatgaacca
    15541 cttggagcac ttgatttaaa gttaagacaa gagatgcaaa tagaattaaa gagaatccaa
    15601 aaaaaattag gaataacatt tgtttttgta actcatgacc aagaagaggc tcttactatg
    15661 tcagatacta ttgttgtaat gtataagggc aaaattcaac aaatgggaac accacaagat
    15721 atatacaatg aacctaaaaa ttcatttgta gctaaattta taggtgaaag taacatattt
    15781 gatggaatta tgatagaaga ttataaagtt aatttctgta atagagattt tgagtgtgtt
    15841 gataaaggat tcgaaaaaaa cgaaaatata gaagtagtaa taagaccaga agatataaaa
    15901 atggtaaagc cagaagaagg aatgttaaag gggaaagtta catctacagt gtttaaagga
    15961 gtgcattacg agatagaatt gaatgaaaat ggaagattgt ggatacttca taatactaaa
    16021 aatgctgagg ttgggactga actaggcatg gatatatatc cagaagatat ccatataatg
    16081 agaaaagaga ataactaaga tgaaaagaaa atctttttta gcatatccat atgtagtttg
    16141 gagtgcgata tttgtaataa ttcctttaat attagtagta ttttttagtt ttacaaaaga
    16201 atcaggtgga ggttatgcct ttacacttga gaattataaa gaagtaattg accctatata
    16261 tatgaaagtt tttgggcgtt ctattctttt agcaggagga gctacattaa tatgccttat
    16321 agttggttat ccagtggcat atataatttc aaaggctcgt gtaagtagaa gaggttcatt
    16381 aatattattg tttattcttc ctatgtggat gaactttctt ttaagaacat atgcatgggt
    16441 tgctatatta ggaaaaaatg gacttttaaa tacattttta ggttggtttg gaatacaacc
    16501 attagcaatt ttgtatacta attttgctat acttttaggt atggtgtata actttttacc
    16561 atttatggtt cttccaatat atacagcact atcaaagatg gataatgatt tgataaacgc
    16621 tgctcatgac ttgggtgcaa ataatatgac agtttttaga aaaattatat ttcctttgag
    16681 tttgccaggt gtaatgtctg gaataactat ggtatttatg ccagcagtca ctacttttgc
    16741 aatatcaaga ctgttaggtg gaggtaaaat aatgctggtt ggagatttga ttgaacaaca
    16801 gtttacagtt gttggagatt ggaactttgg ttcagctata tcgatattta tgatgatagt
    16861 aatattaata tctatgtcta taatgtcaaa atttggagat gaatctgaca aagaaggcgg
    16921 tgggttacta ttttaaaatt aaagaaaata acttctaata tatatttagc attagtattc
    16981 ttatttttgt atgcacccat atttgctttg gtactatttt ctttcaatga ttcaaaatca
    17041 atggcaagat ggaatggatt tacatggaag tggtatggtc aattacttca aaatgagagt
    17101 ataatgagtg ctttatatta tactattgta atagctatat tagcgtctgt aatatctaca
    17161 atagtaggaa ctataagtgc tataggaata cataagatga gaggtaagag taagaaatta
    17221 atactaaatg taaactatct tcctatttta aatacagaga tagttacagc agtagctctt
    17281 atgagtctgt ttgtatttgt aaaaatggaa tttggattta caacaatgct attggcacat
    17341 ataatgtttt gtttgccata cgttatactt tcagttttgc caaaaatgaa gcaattacct
    17401 gataatatag aagatgcagc tatggacttg ggagcaacac ctatatacgc tcttagaaaa
    17461 gtaattttac cacaaataaa accaggtata gtttctggat ttttaattgc atttactatg
    17521 tcaatagatg actttataat aagtttcttt aatgctggaa atggtgtaag caatctttct
    17581 atagaaatct atggaatggc tagaagaggt ataaaacctg aaataaatgc attatcaaca
    17641 ataatgtttg cagtggttct aggtttactt ttactggcta ataaaaaaga atctatagta
    17701 agggggatta aataatgatt aagtttaaaa aagtaatatc ccttatagct gtaggtatga
    17761 tgacagttac tttatttaca ggatgtaata aagcaactga ttctacaaaa gttttaaatg
    17821 tatataatgt tggagattat atagatgagt ctcttataga taagtttgaa aaagaaacag
    17881 gaatagatgt tcaatattca acttatgata ctaatgaaat gatgtatcaa aaagtaaaga
    17941 gtggtagtac acactatgat ttagtatttc cttcagacta tatggtagag aaaatgaaaa
    18001 atgaaggtct tttggaaaaa ttagatttta agaatatacc taatatgaaa tatttagata
    18061 agagtttttt aaatcctgta tacgacaaaa ctaatgaata tagtgttgca tatatgtggg
    18121 gaactcttgg aatattgtac aataagaaag aagttaaaga ccctatggat agctggaata
    18181 tactttggaa tccaaagtat aagggaaata taatgatgtt tgactctgtt agagatacta
    18241 tagggataac tcttaaaaaa ttaggatata gcatgaatag tgtaaatcca aaagaaataa
    18301 atgaagctaa aaatctttta atgaaacaaa aggatttagt attggcttat gtaaatgatg
    18361 aaggtaagga tagattgctt ggtggagaag tggcaatggg tatgctgtat tctggagatg
    18421 cagttacact aatggagcaa aatcctgacc ttgattatgc tatacctaaa gagggaacta
    18481 ataaatgggt agatgctatg tgtgtaccta caacagcaca aaataagaaa gaagctgagc
    18541 tgtttataaa ctttttatta gacccagaga atgcaaaagt aaatgctgag tacattgggt
    18601 attcaacacc taatacagga gcactaaaat tattagaccc agaaataact cagaatcctg
    18661 ttgcatatcc atctaaagaa gtacttgata aatgtgaaac ttttgaagat attggtgaaa
    18721 atataaaatt atatgacaag gcttggatag agttaaaatc aaaatagggt cttgttgttt
    18781 taagtaaatt tacttaataa atacttaata aaactgttct tttttagaaa atttattttc
    18841 taattattga acagttttat tttttgttta agttatatta atatgcaatt taaatagttt
    18901 attaataaat gaaaacttat ttgtataatg actttaataa taattagaaa aaatgggcaa
    18961 cttaatagaa taattggagg tgtaaattta taaaaaatga ataggataaa taaaaaaata
    19021 gatttattaa tattggcttt gctcgttttt atatttttaa ttgttggaat agtaatacta
    19081 tcgattagga cagaaaataa attgaatgtg ttcataatgt tagcaataat tttttttata
    19141 ataatgttaa cttatttatc aaattcagta gtggggttga taacaagttc aataattata
    19201 tttatgtata catcctatat tttatataac aatattactc acaatatgga tgttgaattt
    19261 atatcatata tgtggataat agcaacacca gtatcttcaa ttataatggg taatctgaac
    19321 aaaagcataa atgaattgca aaatacaaat aaaaaactat ctgaacagta taaggaatta
    19381 gtgactattg atagtgaaac tggacttaga aatttaaaga ttttttataa tgatgtaaat
    19441 atggagataa gtaaatctat tagacataat actgattttt ctcttatgat agtaaaatta
    19501 ccatactacg gaaacctaca gactatattt ggtgaaaaca aaactaataa aatagtaaaa
    19561 tatattggtt caaatattat agaatgtaca agaaatgaag atataattta tagtttacaa
    19621 aaagatatga tagggatact aatgccaaat acatctttgg aaggttcaaa agttgttaaa
    19681 gacagaataa agaaaaggat aaaagaatta aatttagatt taaacaatag gggaaaatat
    19741 gtaaatatag atgtcaaaat tgcattttta caatataaga gtagttttgg agatagtata
    19801 aattttaaga atatagttga agaggaactt caatatgatg tttaaaatat ttaataaaag
    19861 tctattaaaa ttagtagttg tattatcaat agtactgaca atatcaaatt ttagcttatc
    19921 ttcatatgca tcagaaatta cttccagcac agataaggta ttaatcatat atgattctaa
    19981 gaaagaaaca gcttataata gagatatttt aaatattatg aggactctat tgggaagatt
    20041 tagttcagat atagagctat taaaattgag taactatgat ggtgagataa ataaaaatta
    20101 ttacagtcat atatttattt taggaatcaa tgaaaactca tataataatg ataaaaacac
    20161 taaaaacttg attagttcct taaattcata taaaggaact atatgttggt taggatatgg
    20221 tatagaaaat cttttggaac ataaaaaata caatttagac tatgtaggaa aaacaaacaa
    20281 tatagttagt gtaaattata gaggtaaatc atataacttg gatgaacatt atgtttttaa
    20341 cattgtagaa tcaaaagata caagtaataa agtaatagga agtattaatg ataccttaaa
    20401 taagtatcca tatataataa atgataaaaa tcttttttat gtatcaaaat tagatttaga
    20461 tggagtttta ttttatatat tttgcgattc acttaatgat atttttaata ttaagacgtt
    20521 tgataaagga cgtatatttg taagaataga agatgtacat gcttttagag aaccaaaaaa
    20581 tcttgtagag atagcggatt atttaagtag taagaatata ccatttacga ttgcattaat
    20641 accagcatat gtaaatccta aaaatcataa ggtaataact ttatcagaaa gccctgaaat
    20701 tgtcaaagca ataaaatata tgcaagataa aggtggaact gtgatattgc atggatatac
    20761 tcatcaatac aagaaagaag aagtttctgg agaagggtat gaattttggg atggtaaaaa
    20821 agatgaaccc ttaaaagaaa atatgaagat atttgtcaaa gatagagttt tgaatggtct
    20881 tagagtatgt atagagaatg gaatttatcc tcttgcattt gaagcgccac attatgcaat
    20941 ggaatcagag ggatataaag aattaaaaaa atatttttct acttatatgg ggcaacatca
    21001 aaataatgat aaaaaattta gcacaaatac atatccttat attattaggg atacagagga
    21061 gtttaatata tttatacccg aaaatttagg atatatagac ccagaagata agtttacttt
    21121 tcaaaatata aaagaaaatt taaataaatt atccattgta agaggtttta gtggagggtt
    21181 cttctttcat tcatatttaa acatagagta tctaaaaaac acaatagaat atttagaaaa
    21241 acagaatata gaatttatga atttaagaga ttttaataat tgggtaaaag ttgatgaaat
    21301 acaaataaga aataatggag atgaaattat agtcaactat gataaggact tagatgaact
    21361 aactaaaagt gatactagat ttaaatccat atccaatatt agtaaaattt taatattcat
    21421 agtatcgata agtgttttaa tatttgtgat tatatttata tattttaaga gaatagataa
    21481 aaagaaattt ttaaaataaa tgattttata ttagaatcaa tattttaaaa tatattagga
    21541 gaaatttatg agagatttaa atgtatcag
//
LOCUS       DAELJO010000052        21094 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELJO010000052 DAELJO010000000
VERSION     DAELJO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.052"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..237
                     /gene="tuf"
                     /locus_tag="KPB88_001633"
     CDS             <1..237
                     /gene="tuf"
                     /locus_tag="KPB88_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0053915.1"
                     /translation="GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            609..920
                     /gene="rpsJ"
                     /locus_tag="KPB88_001634"
     CDS             609..920
                     /gene="rpsJ"
                     /locus_tag="KPB88_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG0053916.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1012..1641
                     /gene="rplC"
                     /locus_tag="KPB88_001635"
     CDS             1012..1641
                     /gene="rplC"
                     /locus_tag="KPB88_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG0053917.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1671..2294
                     /gene="rplD"
                     /locus_tag="KPB88_001636"
     CDS             1671..2294
                     /gene="rplD"
                     /locus_tag="KPB88_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG0053918.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2294..2584
                     /gene="rplW"
                     /locus_tag="KPB88_001637"
     CDS             2294..2584
                     /gene="rplW"
                     /locus_tag="KPB88_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG0053919.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2614..3444
                     /gene="rplB"
                     /locus_tag="KPB88_001638"
     CDS             2614..3444
                     /gene="rplB"
                     /locus_tag="KPB88_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG0053920.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3479..3760
                     /gene="rpsS"
                     /locus_tag="KPB88_001639"
     CDS             3479..3760
                     /gene="rpsS"
                     /locus_tag="KPB88_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG0053921.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3792..4127
                     /gene="rplV"
                     /locus_tag="KPB88_001640"
     CDS             3792..4127
                     /gene="rplV"
                     /locus_tag="KPB88_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG0053922.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4150..4965
                     /gene="rpsC"
                     /locus_tag="KPB88_001641"
     CDS             4150..4965
                     /gene="rpsC"
                     /locus_tag="KPB88_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG0053923.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5001..5432
                     /gene="rplP"
                     /locus_tag="KPB88_001642"
     CDS             5001..5432
                     /gene="rplP"
                     /locus_tag="KPB88_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG0053924.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5434..5637
                     /gene="rpmC"
                     /locus_tag="KPB88_001643"
     CDS             5434..5637
                     /gene="rpmC"
                     /locus_tag="KPB88_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG0053925.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5661..5915
                     /gene="rpsQ"
                     /locus_tag="KPB88_001644"
     CDS             5661..5915
                     /gene="rpsQ"
                     /locus_tag="KPB88_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG0053926.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5941..6309
                     /gene="rplN"
                     /locus_tag="KPB88_001645"
     CDS             5941..6309
                     /gene="rplN"
                     /locus_tag="KPB88_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG0053927.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6333..6638
                     /gene="rplX"
                     /locus_tag="KPB88_001646"
     CDS             6333..6638
                     /gene="rplX"
                     /locus_tag="KPB88_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG0053928.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6668..7210
                     /gene="rplE"
                     /locus_tag="KPB88_001647"
     CDS             6668..7210
                     /gene="rplE"
                     /locus_tag="KPB88_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG0053929.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7228..7413
                     /locus_tag="KPB88_001648"
     CDS             7228..7413
                     /locus_tag="KPB88_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG0053930.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7446..7844
                     /gene="rpsH"
                     /locus_tag="KPB88_001649"
     CDS             7446..7844
                     /gene="rpsH"
                     /locus_tag="KPB88_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG0053931.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7875..8417
                     /gene="rplF"
                     /locus_tag="KPB88_001650"
     CDS             7875..8417
                     /gene="rplF"
                     /locus_tag="KPB88_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG0053932.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8453..8821
                     /gene="rplR"
                     /locus_tag="KPB88_001651"
     CDS             8453..8821
                     /gene="rplR"
                     /locus_tag="KPB88_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG0053933.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8842..9351
                     /gene="rpsE"
                     /locus_tag="KPB88_001652"
     CDS             8842..9351
                     /gene="rpsE"
                     /locus_tag="KPB88_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG0053934.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9366..9551
                     /gene="rpmD"
                     /locus_tag="KPB88_001653"
     CDS             9366..9551
                     /gene="rpmD"
                     /locus_tag="KPB88_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG0053935.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9584..10027
                     /gene="rplO"
                     /locus_tag="KPB88_001654"
     CDS             9584..10027
                     /gene="rplO"
                     /locus_tag="KPB88_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG0053936.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10071..11339
                     /gene="secY"
                     /locus_tag="KPB88_001655"
     CDS             10071..11339
                     /gene="secY"
                     /locus_tag="KPB88_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG0053937.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11356..12006
                     /locus_tag="KPB88_001656"
     CDS             11356..12006
                     /locus_tag="KPB88_001656"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG0053938.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12007..12753
                     /gene="map"
                     /locus_tag="KPB88_001657"
     CDS             12007..12753
                     /gene="map"
                     /locus_tag="KPB88_001657"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG0053939.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12775..13044
                     /locus_tag="KPB88_001658"
     CDS             12775..13044
                     /locus_tag="KPB88_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG0053940.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13082..13300
                     /gene="infA"
                     /locus_tag="KPB88_001659"
     CDS             13082..13300
                     /gene="infA"
                     /locus_tag="KPB88_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG0053941.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13336..13449
                     /gene="rpmJ"
                     /locus_tag="KPB88_001660"
     CDS             13336..13449
                     /gene="rpmJ"
                     /locus_tag="KPB88_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG0053942.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13602..13973
                     /gene="rpsM"
                     /locus_tag="KPB88_001661"
     CDS             13602..13973
                     /gene="rpsM"
                     /locus_tag="KPB88_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG0053943.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14009..14407
                     /gene="rpsK"
                     /locus_tag="KPB88_001662"
     CDS             14009..14407
                     /gene="rpsK"
                     /locus_tag="KPB88_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG0053944.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14439..15062
                     /gene="rpsD"
                     /locus_tag="KPB88_001663"
     CDS             14439..15062
                     /gene="rpsD"
                     /locus_tag="KPB88_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG0053945.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15140..16087
                     /locus_tag="KPB88_001664"
     CDS             15140..16087
                     /locus_tag="KPB88_001664"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG0053946.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16108..16449
                     /gene="rplQ"
                     /locus_tag="KPB88_001665"
     CDS             16108..16449
                     /gene="rplQ"
                     /locus_tag="KPB88_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG0053947.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16569..17402
                     /locus_tag="KPB88_001666"
     CDS             16569..17402
                     /locus_tag="KPB88_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0053948.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17390..18256
                     /locus_tag="KPB88_001667"
     CDS             17390..18256
                     /locus_tag="KPB88_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0053949.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18250..19053
                     /locus_tag="KPB88_001668"
     CDS             18250..19053
                     /locus_tag="KPB88_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG0053950.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19085..19816
                     /gene="truA"
                     /locus_tag="KPB88_001669"
     CDS             19085..19816
                     /gene="truA"
                     /locus_tag="KPB88_001669"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0053951.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19936..20367
                     /gene="rplM"
                     /locus_tag="KPB88_001670"
     CDS             19936..20367
                     /gene="rplM"
                     /locus_tag="KPB88_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG0053952.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20396..20788
                     /gene="rpsI"
                     /locus_tag="KPB88_001671"
     CDS             20396..20788
                     /gene="rpsI"
                     /locus_tag="KPB88_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG0053953.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8427 a         2484 c         4386 g         5797 t
ORIGIN      
        1 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
       61 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      121 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      181 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaatcc
      241 tttactaaac taatggattt aatataaaaa aagagggggg taactcctct cttttttgtt
      301 ttttgttgac aagtagcgct aaagcatata tactaaacta gtgccttgag acagattaac
      361 aatcaagttt aaggttatga tgttgtaata gaaaataaaa ttgaaaaaaa cagaaaaaaa
      421 acttgaaaaa atgtaacaga tgatataaaa tataatagtg tgcttgagag attaagaaaa
      481 taataattaa atcctagtga aatacgaact gatatcgatt taaaagttac aacacacccg
      541 gagcagtgta tcggtattgg ttgtacgcaa cttgaaaatt aaacacgtga taaaggaggg
      601 aaaacaaaat ggctaagaat gaaaaaataa gaataagatt aaaatcatat gatcacaaat
      661 tattagattt ttcagcaggt aaaatagttg aaactgctaa aaaggctgga tcacaagttt
      721 caggacctgt gcctctacca acagaaaaac aagttgtaac tatattaaga gctgtacata
      781 agtacaaaga ctctagagaa caatttgaaa taagaactca taaaagacta atcgacatag
      841 ctaatccaac acctaagaca gttgactcat taatgagatt agacttacca gcaggtgtag
      901 atatagaaat aaaattataa aaacccctaa gatattatcc tatagatgat tatcttagaa
      961 tgattactct aaaagatgta atccgctgta agaattatag gaggtgctaa tatgaaagga
     1021 atattaggca aaaaagttgg tatgactcaa atattcactg ataaaggtgt agttatacct
     1081 gtaacagctg ttgaagcagg gcctatggtc gttactcaaa taaaaacagt tgataaagat
     1141 ggatacaatg ctatacaaat aggttttgaa gatgctaaag aaaaagcttt aaacaaacct
     1201 aaaaaaggac atttagctgc tgctaatgtt ttaaagaaac atttaaaaga atttagagta
     1261 gattctgtag aaggatacac agtaggacaa gaaataaaag ctgatgtatt tgaagcaggt
     1321 gcaaaaatag atgttactgg aataagtaaa ggtaagggat tccaaggtcc aataaaaaga
     1381 catggacaaa gtagaggtcc tgaaactcac ggttctagat accacagaag accaggttca
     1441 atgggagctt gttcttaccc aggtagagta tttaaaaaca aaaaattagc tggacatatg
     1501 ggaagcgtaa aggtaacagt tcaaaactta gaggttgtta aagtagatgc tgacaagaac
     1561 cttatattag ttaaaggagc tataccagga gctaaaggtt cagtagtaac tataaaagaa
     1621 gctataaagg tttctaaata atgactaacc tagagaaagg aggaataaca atgccaaaat
     1681 taaatgtact aaatgttagt ggacaaaacg ttggagaaat agaattatca gattctatat
     1741 ttggcgtaga agttaatgga catgttttat acgaagttgt aaaaaatcaa ttagcaaata
     1801 agagacaagg aactcaatct gctaaaacta gagcagaagt tagaggtggc ggaagaaaac
     1861 cttggaaaca aaaaggaaca ggtagagcaa gacaaggttc tacaagatct gtacaatggg
     1921 taggtggagg agttgctttt gcacctaaac caagaagcta caaatataca ttacctaaga
     1981 aggtaagaag attagctatg aagagtgctt tatcttcaaa agttcaaaat agtgaagtta
     2041 tagtattaga tgcattaaac atggatgctc ctaagactaa agaatttgct caaatattaa
     2101 acaatataaa tgctgctaaa aaagctttag tagttatagc tgacaaaaac gataacgtaa
     2161 taaaatcagc tagaaatata gaaggtgttc aaactgcatt agttaatact atgaatgtat
     2221 atgatatatt aaaatacgat tcatttataa taactacaga tgctgttaaa aaagtggagg
     2281 aggtgtacgc ataatgacta atccacatga tgtaataata agaccagttg taactgagca
     2341 cagtatggct gaaatgggtg aaaaaaaata cacttttgtt gtggctaaag atgcgaacaa
     2401 aactgaaata aaaaaagcag tagaaaaagt attcggagtt agtgttgata aagtaaatac
     2461 tttaaactac gatggaaaag ttaaaagaat gggtagaact caaggaagaa catcaagctt
     2521 taagaaagca gtagttaaat taactgctga tagtaaagaa atagaattct tccaaggaat
     2581 gtagtattat aacaaacgaa ggagggaaaa cagatggcta taaaaaagtt tagaccaact
     2641 tctcctgcct taagacaaat gacagtttta gtttctgatg aaataacatg taatcaacca
     2701 gaaaaatctc ttttagttaa cttaaagaaa aatgctggta gaaacgtaca tggtagaata
     2761 actgttcgtc atagaggtgg aggacaaaaa agaaaatata gaataataga ttttaaaaga
     2821 gataaagatg gtatacctgc taaggttgct actatagaat atgatccaaa cagaactgca
     2881 aatatcgcat tattaaacta tgctgatgga gaaaaaagat atatattagc tccagttgga
     2941 attaatgttg gagatactat attatcagga ttaggtgctg atataaaacc aggaaactgt
     3001 ttagcattaa aagatatgcc agttggtact atcattcata atatagaatt aaagccagga
     3061 aaaggagctc aattagttag atctgctgga gtttctgcac aattaatggc taaagaagga
     3121 aaaaatgctt tattaagatt accatcaggt gagatgagat tagtaagcat aaactgtaaa
     3181 gctactatag gtcaagttgg aaatatagag catggtaacg tagttatcgg taaagcaggt
     3241 agaaaaagac atatgggaat aagacctact gtaagaggtt ctgtaatgaa ccctaatgac
     3301 catccacacg gtggagggga aggtagatct ccaataggta gaccatctcc tgttactcca
     3361 tggggtaaac cagctcttgg atacaaaact agaaagaaaa ataaagcttc aaataaacta
     3421 atagtatcaa gaagaactaa gtagtaaatt tatttttgct cggaaaggag gaatataaat
     3481 gtcaagatca actaaaaaag ggccttttgt ccatgcaaga cttttaaaga agatagaagc
     3541 tatgaatgct agtggaaata aagaagttat aaaaacttgg tcaagatctt caactgtatt
     3601 tccacaaatg gtagaaaaca ctatagctgt tcatgatgga agaaaacatg ttccagtata
     3661 tataacagaa gatatggttg gacataaatt aggtgagttc gttcctacta gaactttcaa
     3721 aggacataag gatgacgaaa aatctaataa gagaaaataa ttaaattatg actaagaaag
     3781 gaggaataac aatggaagca aaagctactg ctaaatacgt acgtgtatca ccaagaaaag
     3841 caggccaaat atgtgacctt gttagaggaa agaatgttga tgaagcatta gcaatattaa
     3901 agtttactcc aagaggagca gcttcaataa tagctaaggt tgtaaaatca gctaaagcta
     3961 acgcagaaaa caatcacgaa atggatactg aaaaattata tatagcatca atagttgcta
     4021 atcaaggacc aacaatgaag agattcatgc ctagagctat gggtcgtgca actacaataa
     4081 gaaaaagaac ttctcatata gaggttgttg ttaaagaaaa aaaataatta atagatagga
     4141 gggaaagtaa tgggtcaaaa ggttaatcca cacggcttaa gggtcggtgt tataaaagat
     4201 tgggactcaa gatggttcgc tactgataaa aaagagttcg gaaacttatt attagaagac
     4261 cataatatac gtaaattctt aaagaaaaga ttatactcag ctggagttgc taagatagaa
     4321 atagaaagat cagctaacaa aataaaaatg gacttacacg ttgctaagcc tggtgtagtt
     4381 ataggaagag ctggtgcagg aatcgaagca ttaaaagctg aattagaaaa aatgacaaag
     4441 aaaactataa tagttaatat agtagaagta agaagtacag ataaaaatgc tcaattagta
     4501 gctgaaaata tagctttagc tatagagaga agggttgctt tcagaagagc aatgaaacaa
     4561 gctatacaaa gagcaatgaa atctggtgct aaagggataa aagtttctgc ttcaggaaga
     4621 ttaggtggag ctgaaatggc tagaactgaa ggttatagtg aaggaaatgt gccactacaa
     4681 actttaagag cagatataga ttatggtttc gctgaagctg atacaactta tggaaaaatc
     4741 ggtataaaag tttggatatg taatggtgaa gttttaccaa ctagagacgg tgtaaatcca
     4801 agagaagaaa gcagaaagag cgatagaaga gacaacaaga gagataatag aagaaacgat
     4861 agaagaggaa atgacagaag aggaaatgat aacagaggaa actacagagg acaaagacct
     4921 caaggtggat caagacctca aagaactgaa aacaaaggaa attaataata gttaggttca
     4981 aggaaggagg aaaaatactc atgttaatgc caaaaagagt aaagcgtcgt agagttcata
     5041 gaggaagcat ggctgggcaa gctcataagg gtaataaagt tacttatggt gagtttggat
     5101 tagtagcatt agaagcttct tggataactt ctaatcaaat agaagctgcc agaatcgcga
     5161 tgactagata tataaaaaga gggggaaagg tttggataaa aatattccct cataaaccag
     5221 taacaagaaa accagcagag actcgtatgg gtgcagggaa aggttctcca gaatattggg
     5281 tagctgtagt taagccagga agagttatgt ttgaattagc aggtgtttct gaagataaag
     5341 caagagaagc tatgagactt gctgcacata aattaccaat caaatgtaaa tttgttaaaa
     5401 aagaagattt agaagtaaag ggtggtgaat aggatgaaag ctaaagaatt aagagattta
     5461 acaagcgaag agctaatgaa caaattaaac gacttcaaaa gtgaattatt tagcttaaga
     5521 ttccaattag ctactggtca attagaaaat acagctagaa taaagtttgt taagaaggat
     5581 atagcaaaag ttaaaactgt tcttgctgaa agaaagttat acgaaactag agcttaattt
     5641 ggaaaggagg ctttttaaac atggaaagag gaagaagaaa agttagaata ggccgtgttg
     5701 ttagtgataa gatggataaa acaatagtag tagctgttga agaattcgta cgtcatccac
     5761 tatataataa acgtgttaag agaactaaga aattcaaggc tcatgatgaa aagaatatat
     5821 gtaacatcgg agatagagtt aaaataatgg aaactagacc attatctaaa gataagagat
     5881 tcagactagt tgaagttgtt gagaaagtta agtagttttt gaagaaggag ggattacgat
     5941 atgatacaac aagaatcacg tctaagagtt gctgataact caggagctaa agaactttta
     6001 tgtatacgtg ttctaggcgg aagtaaaaga agatatggta acataggcga cgttatagtt
     6061 gctactgtta aaagtgcaac acctggtgga gttgtaaaaa aaggtaaggt tgttaaagct
     6121 gttatagtaa gaagcaaaca aggcgtaaga cgtaatgacg gaagctatat atcttttgat
     6181 gaaaatgctg ctgttataat aaaggacgat aaaactccag taggaactcg tatattcggg
     6241 cctgttgcta gagagttaag agacaatgaa ttcatgaaaa tagtttctct tgctccagaa
     6301 gtactataat taaggaggtg caataggaca tgatgcgtgt taaaaaaggt gacactgttg
     6361 tagttatagc aggtaaagat aaaggtaaaa aaggttcagt tttaaaggta tatcctaaaa
     6421 ctagcaaagt gttagttgaa ggtgtaaatg taataactaa acatcaaaaa ccaagtgcta
     6481 tgaaccaaca aggtggaata ataaataaag aagccccaat acacatatct aatgtaatgc
     6541 catttgatcc tgaaacagga aaaggcgtta gagtaagata tgaagtaaaa gatgggaaca
     6601 aagtaagagt atcagcaaag agcggaaaag aattataata aatattgctt gaaaggaggg
     6661 accttaaatg gcttctagat tacaagaaaa atacatgaaa gaagttgctc ctgctttaat
     6721 ggagaaattt ggatacaaaa acgtaatgga gatacctaag ttaaataaaa tagttattaa
     6781 catgggtata ggtgacgcta gagaaaatcc aaaaggatta gaaaaaggcg ttgaagaatt
     6841 agaaatgata tcaggacaaa agcctgttat aactaaagct agaaaatctg ttgctaactt
     6901 caaattaaga gaaggaatgc caataggaac aaaagttaca ttaagagctg acaaaatgtt
     6961 ctacttcatg gacaaattag tttcagtttc tttaccaaga gttagagact ttagaggagt
     7021 taaccctaat gcttttgatg gtagaggaaa ttatgcttta ggagttaaag aacaattaat
     7081 attccctgaa atagaatatg ataaaataga taaagtaaga ggaatggata taatatttgt
     7141 tactacagca aaaactgacg aagaagctcg tgaattatta aaattattag gaatgccatt
     7201 ttctaagtaa gcaaaggagg gattcccgtg gctagaaaag cgatggttgt taagcaacaa
     7261 agaaagcaaa agtacgctac tagagaatac actagatgta ctatatgcgg aagaccgcat
     7321 tcagttttga aaaaatttgg tatatgccgt atatgcttta gagaattagc ttataaaggt
     7381 caaatacctg gtgttagaaa agcaagctgg tagaagcttg attagaatgg aaggagggca
     7441 acaaaatgac aatgacagat ccaattgcag atatgcttac tcgtataaga aatgctaatg
     7501 ttgttaagca tgaaactgta gatgttccag cttctaatat gaagaaagaa ttagctagaa
     7561 tcttattaga agaaggtttc ataagaggtt atgatgttat agaagatgga aaacaaggta
     7621 tcataagaat acaattaaag tacggacaag aaggcgaaag agttataaca ggtcttaaga
     7681 aaatatctaa gcctggaatg agagtttacg ctgctaacca tgagatacca aaagtattaa
     7741 acggattagg aatatcagtt atatctactt caaaaggaat attaactgac aagcaagcta
     7801 gaaaagaaaa tgttggtgga gaagtaatct gctacgtttg gtaattaata tgaacaatgt
     7861 aaggaggtgc gaccatgtca agaataggtg ttaagccaat aataatacca gcaggtgttg
     7921 aagttactat agctgaagga aatttagtta cagtaaaagg tccaaaagga actttaacta
     7981 aacaattaag tgctgagtta aacataaaaa aagaagaaaa tacaataatg gttgaaagac
     8041 caactgataa taaaaaacat agatctttac acggactaac tagaacttta ttagataata
     8101 tggttgtagg tgtaaatact ggtttcgaaa agaaattaga attaaaaggt gttggatata
     8161 gagctcaaaa acaaggaaag aaattagtta tgaacttagg tttctctcat ccagttgaga
     8221 tggaagatcc agaaggaata actgttgaag ctccaaacca aacagagtta atagtaaaag
     8281 gtatagacaa gcaattagta gggaattatg ctgctaagat aagagcttgg agaaagccag
     8341 agccatacaa aggtaaaggt ataaaatacg ttgatgaagt aatcagacgt aaagaaggaa
     8401 aaactggtaa aaaataatac caaaacttaa acgctagaaa ggagtgagct aggtgttaaa
     8461 aaaggctgat aaaaatgcta atagacttca aagacataag agagttcgta gaaaaataag
     8521 cggtacttct caaagaccaa gattatgtgt atttagaagt gctaacaata tatatgctca
     8581 aataatagat gacacaaaaa gagtaactct agttgctgca tcttctttag aagcagaagt
     8641 taagagtgct gtcaatcata caggaaacaa ggaagcagct aagaaagttg gagaattagt
     8701 tgctaaaaaa gctgttgaaa aaggaatcac tgaagttgta tttgacagag gtggatactt
     8761 atatcatgga agaattcaag aattagctga aggtgctaga gaagctggtc ttaagttcta
     8821 atacgaagga gggaaaaata catgctacgt cgtaagccga tagatgcagg acaacttgac
     8881 ttacaagaaa aagttgttga agtaagacgt gttactaagg ttgtaaaagg tggtagaaat
     8941 ttcagatttg cagcattagt agttgttgga gatgaaaacg gacacgttgg tataggtgct
     9001 gggaaagcaa tggaagtacc agacgctata aaaaaagcag ttgaagatgc taagaaaaat
     9061 ctaatagtag tgccaatagt tgggactaca attcctcacg aagtacgcgg acactttggt
     9121 gctggaaata tattaataat gcctgctgta gaaggtacag gagtaatagc tgggggacct
     9181 gctagagccg tacttgagct agctggacta aaagacgtta gagctaaatc tttagggtct
     9241 aacaatccaa gaaatatggt aaatgctaca atagaaggac ttaattctct aaaaacagtt
     9301 gaagatatag ctaaacttag aggtaaaaaa gttgaagaac ttctagggta aggaggtagt
     9361 gtaaaatggc taaattacaa ataaagttag ttagaagtgt tataggaact actcctaacc
     9421 aaaaaaagaa tgttgaagcg ttaggattaa gaaaaagaga acaagttgtt gtaaaagaag
     9481 acaatgctca aacaagaggt atgataaata aagtaagtca cttattagaa gtaactgaaa
     9541 tagctgaata atttttataa agtagtgcaa ggaggtgcaa tccatgaagt tacatgagtt
     9601 aaaacctgct gaaggtgcag taagagctaa gagaagatta ggtagaggta ctgcaactgg
     9661 acaaggtaaa actgcaggac gtggacaaaa aggtcaatgg tctcgttctg gtggtggagt
     9721 aagagtagga tttgaaggtg gacaaatgcc actagctaga agacttccta agagaggttt
     9781 caataacata tttaagaaag tttacacaga agttaatgtt gaagttttaa acagatttga
     9841 aaatggaaca gaaataactg cagaattatt aaaatctact aaaactataa gcaaaatagg
     9901 taaagacgga attaaaatct taggtgaagg taatttagag aaagctttaa ctgttaaagc
     9961 tgctaaattt acagcttcag ctcaagaaaa aatagaaaaa gctggtggaa aagcagagtt
    10021 agtataataa cctgctaatc tcctagccag tacaggcggg gtgaattagc gtgctgtcaa
    10081 aattaaaaca agcttggaag attaaagcag taagaaaaaa agtaatgtat acacttatga
    10141 tgatcgtgat attcagaata ggtactacta taccagttcc aggcatagac actagtatta
    10201 tacaaaagat ggtaggcggc aatagtctac tttctctgta taatatgttt actggtggag
    10261 catttagtaa cttttcacta tttgctttag gtataagtcc atatatcact gcatcaatca
    10321 taatacaact tctaacagta ggttttgaaa gtttagcaga acttcaaaaa tctggtgaag
    10381 aaggaaagaa aaaaatcaat aagtacacta aatatacagc actagctcta gcagttgtac
    10441 aagctttggg aattacatta ggaattgtaa gaagtgcttt aatatcaaat agtgtgtttt
    10501 ttatcacaac tgttgtaata acattagttt cagcaagtat gttagtaatg tggattgggg
    10561 acaaaattac tgaaaaagga atcggtaatg gtagttcagt aataatattt gcaggtataa
    10621 tatcaagaat acctacagat gttattaaaa tatcgcaaca agttaaatct ggcgaagttg
    10681 caccttgggt tatagtgatt ctagctgtag ttatattact tacagtaaca ggagttacat
    10741 ttatacaaga agcaactaga aagatacctg tacaatatgc taaaagagtt gttggaagaa
    10801 aaatgtatgg aggacaaagt tctcatatac caatgaaagt taaccagtca ggagttatgc
    10861 ctataatttt tgctagttca cttttagctt tccctcaaac aatagctatg tttatgggtc
    10921 caaatgctca ggcttttgtt caaaaatact taagtatggc aacagaacaa ggattttgga
    10981 catatagatc aattgagatt ctacttataa ttttcttctc atatttttat acaactgtat
    11041 catttaatac agaagacata tctaaaaata tgaaaaataa tggtggattt ataccaggta
    11101 taagaccagg tgagcctact atggattatt taaatagaat tttaactaga ttaactttag
    11161 ctggagctac tttcttagca ataattgcta tggtgccagc tcttacaaca cattatatga
    11221 aagtaaatat gagtctagct gggacttcat tgctaatcgt tgttggagtt gctttggagc
    11281 ttaagagaca gttagagtca aacttagtta tgagaagcta tcaaggtttc ttaaaataaa
    11341 atggagatgg ataatatgag aataatatta cttggacctc caggtgctgg taaaggtact
    11401 caggcggcag gaatagtaga aaaatacaat atacctcata tatcaacagg agatatattc
    11461 agaaagaata taaaagaggg aacagaactt ggaaaaaaag ctaaagaata catggaccaa
    11521 ggtttattag taccagatga gttaactgta ggtttagtta ctgatagaat atctcaagaa
    11581 gattgtaaaa atggatttat gttagatgga tttccaagaa atgtagcaca aggagaacat
    11641 ttagatatct tcttaaaaaa tgctggtata tcactagata aagttgtcaa tattgaagtt
    11701 gataagagta tattagtgtc tagagcagtt ggtagaagaa tatgtaagtc ttgtggagct
    11761 acttaccatg ttgagtttaa tcctcctaaa gtagaaggtg tatgtgatgt atgccaagga
    11821 gaattatatc aaagagctga tgataatgaa gaaactgtat ctaagagaat acaagtttat
    11881 ttagatgaaa ctaagccttt agtagattat tatagcaaac aaggtataat agcagatata
    11941 aaaggtgatc aagcaataga taaagtattt gaagatattg tcgcagcctt aggaagtgga
    12001 aaataaatga ttatcttgaa atcaaaaaaa gaaatagaac ttttaagaga agcaggtaaa
    12061 atagttgctg acactcatga agttttaaga aaagctattt ctccgggaat atctacttta
    12121 gaattagata aaatagctga agagaatata agaaaataca atgcagaacc ttcttttaaa
    12181 ggatatggag gttttccagg aagtatatgt gcttctatca atagagaggt agtacatggt
    12241 attccaggag aaactatatt acaagaaggt gacattgtaa gtttggatat aggagcctat
    12301 tataaaggat atcatggtga ttccgcgaaa actcacggag taggcatgat atctgaagaa
    12361 gataggaaat tgatagaagt aactagagaa agcttctatg aaggaataaa atttgctaaa
    12421 ttaggatata gactttcaga tatctcacac gcagtacaaa cacacgttga gaaacatggt
    12481 ttctcggtag ttagagattt agtgggtcat ggagttggag caaatcttca tgaagaccca
    12541 caagtaccta attatggact cccaggaaaa ggaccaagac ttagagaagg aatggttatt
    12601 gctatagagc caatggttaa tgcaggtcgt tatcatgtaa agactctatc agatgggtgg
    12661 acgactgtta caatagatgg aaaaaaatca gctcattatg agcatacgat agcaattact
    12721 gaacatgaac ctttgatatt gacaaagttg taatttaaaa agtaggtgaa aaaagtgcta
    12781 tcagataatt taagtatagg tcaagttgtt aaagtttcat taggacgaga taaaggaaat
    12841 ttattctttg tagtcaaaat aattaataat gaatatgtat taatagctga tggaaaaaag
    12901 agaaaacttg acaaacctaa gttaaagaaa gtgaagcact taaaaaaata taattttatt
    12961 aatgatgaag ttagaaaaag agtagtatct ggacaagaga ttacagattc ttttttaaga
    13021 gctgaactta ctaagttaaa ttagataatt taacttattg aaatggaggg ctggttagtt
    13081 aatggccaaa aaagatgtta tagaattaga aggtacagtt tcagaagctt tacctaatgc
    13141 tatgttcaaa gttaaactag aaaatggaca tgagatatta tgccacattt ctggaaagct
    13201 aagaatgaac ttcataagaa ttcttgaagg tgacaaggtg aatgttgaac tttctccata
    13261 tgaccttaca agaggaagaa ttacttggcg taagaagtag actatattaa tatttagtct
    13321 aaggagggat taataatgaa ggttagacca tcagtaaaac caatatgtga aaaatgtaaa
    13381 gtaataaaaa gaaaaggaaa agtaatggtt atctgtgaaa atccaaagca taagcaaaag
    13441 caaggataag aaattattac tagcattttg ttaaatttta tagtataata ctatattgtg
    13501 atgttaacaa aagaacgtta aaatcgttga acaatctggc gtaggattgg catatgatgt
    13561 gaagttttaa acactaaaaa ctatgtgtag gaggtgcgat catggcaaga atagctgggg
    13621 tagatttacc tagagaaaaa agagcggaga taggcttaac ttatatatat ggtataggta
    13681 aagcaactgc taacgaaata ttagctaagg ctgagataaa tccagatact agaataaaag
    13741 atttatcaga agatcaagtt aatgagttaa gaaaagttat agatgacgat ttcttagttg
    13801 aaggtgactt aagaagagaa attgctttaa acataaagag attaagagat ataaaatgtt
    13861 atagaggaat aagacacgct aaaggtcttc cattaagagg acaaagaact aagactaatg
    13921 ctagaactag aaaaggtcct agaaaaacag tatctcgtaa aaagaaaaaa taaataaata
    13981 ataactagat gaaggaggga aaaggaaaat ggctaaacca aaaaagaaag ttacacgtat
    14041 tagaagaaga gaacgtaaaa atatagaacg tggtcatgct catatacaat caactttcaa
    14101 taatacaata ataactttaa ctgacgttca cggaaatgct atatcttggg caagttctgg
    14161 acaattagga ttcaaaggat caagaaaatc aactccattt gcatctcaaa tggctgctga
    14221 aacagctgca aaagctgcga tggaacacgg actaaaaagt gttgaggtat tcgtaaaggg
    14281 gccaggttca ggaagagaag ctgcaataag agctttacaa gcaactggac tagaagtaac
    14341 tatgataaaa gacgttactc caatcccaca taacggatgc agaccaccaa agagaagaag
    14401 agtgtaatta ataagagtac aataggaggt gtagataaat ggcaagatat acaggtgcat
    14461 catgtagaca atgccgtaga gagggaatga aattattcct taaaggtgat agatgttata
    14521 cagacaaatg tgctatagta aaaagaaact atgctccagg tcaacatggc caaggaagaa
    14581 agaaagtttc taactatgga ttacaattaa gagaaaaaca aaaagttaaa agaatatatg
    14641 gagttttaga gactcaattt agaaatttat atgaacgtgc tgaaaatatg cctggtaaag
    14701 caggtgagaa cttattaagt ttattagaga gaagattaga taatgtagtt tacagaatgg
    14761 gactagcatc ttctagaaaa gaagctagac aattagtgac tcatggtcat ttcactttaa
    14821 atggaaataa agtagacata ccttcattaa tagttaaggt tggagatgtt atagaagtta
    14881 aagagaaatc aagatcttct gcaaaattca aaaatttagt agaagttaac tcaagaatag
    14941 ctcctaagtg gttagaagct aatgtagaag gaatgacagc aaaagtagtt ggtgttccaa
    15001 caagagaaga tatagatctt gagatagcag agcacttaat catagaactt tactctaaat
    15061 aataagcaca cttttaaaaa ttattttaaa attttgttta ccctcagtgg attactgaat
    15121 tttaaggagg gttttgtcca tgatagaaat agaaaagcca aaagtagata tagttgagct
    15181 tagcgaagac tatagatatg gtaagtttgt tatagagcct ctagaaagag gatatggaat
    15241 aactataggt aacgcattaa gaagaatatt attatcatca ttaccaggtg tagcagtgaa
    15301 tgctataaaa atagatggag ttcttcatga attttcaaca atacctggtg ttaaagaaga
    15361 tgttactgag ataatattaa ctttaaaaga gctttcagca actatagatg gtgaaggaag
    15421 tagaacactt aaaatagaag ctcaaggacc atgctctatc acaggtgcag atataatctg
    15481 tcctccagat gttgaaatat taagtaaaga tttagcaata gctacattag atgataatgc
    15541 taaacttaat atggagatat ttgtagataa aggtagaggt tatgtttctg ctgaagaaaa
    15601 taaaacagag aatgttccaa taggtgtttt acctgtggat tcaatatata ctcctgttga
    15661 gaaggttagt tatcatgtgg aaaacacaag agttggtcaa aaaacagatt atgataaatt
    15721 agtactagaa gtttggacta atggtagtat aaatcctcaa gagggaatat cattagctgc
    15781 aaaggtatta gttgagcatt taaacttatt tatagactta actgaacatg taagtagtgt
    15841 tgaaatcatg gtagaaaaag aagaagatca aaaagaaaaa gttcttgaaa tgactataga
    15901 agaattagat ttatcagtta gatcatataa ctgtttaaag agagcgggaa ttaatacagt
    15961 tgaagaatta gctaataaat ctgaggatga tatgatgaag gttagaaacc taggtaagaa
    16021 gtcattagaa gaggtaatac aaaaactaga agaacttgga ttgggattaa aaccaagtga
    16081 agaatagcga gcaaaggagg gataagaatg gctaagtacc gtaaattagg acgtgaaaca
    16141 gctcacagaa accttatgtt aagaaactta gtaacttgct tactaagaag tggaagaata
    16201 gaaactacag tgactagagc gaaagaaact cgtagaatgg ctgaaaagat gataactctt
    16261 gctaagagag gagatcttca tgctagaaga caagttttag cttatgttat ggatgaaaca
    16321 gtagttaata acttattcac agatttagct ccaaaatatg ctgagagaaa tggtggatac
    16381 actagaataa taaaaatagg gccaagaaaa ggcgatgctg ctgaaatggc ttttatagaa
    16441 ttagtatagt aaggaaagga ttaggcttga tggcttaatc ttttttttat ttttttgact
    16501 aaggtatgat ataataatgt gtaaaatgat ttttataagt tattgcttac taaaaggagt
    16561 atgagttaat ggataatata gtaaaggtaa ataatatttc gtttgagtat attacagatg
    16621 aagcaaaact taaagcaata gataatttaa gcctagatgt taaaaaagga gaatttgttg
    16681 cgataatagg acataatggt tctggtaagt ctactttatc taaaaactta aatgctattc
    16741 ttatgcctac tgaaggaaat attcttattg atgatatgga tactaaagaa gaagaaagat
    16801 tatgggatat aagacaaact gcaggcatgg tatttcaaaa tccagataat caaatagttg
    16861 ctactatagt agaagaagat gttgcatttg gacctgagaa cttaggtata gaaccaaaag
    16921 aaataagacg aattgtagag gagtctttaa aaagtgtagg tatgtatgat ttaagagata
    16981 gacagcctca tttgttatca ggaggtcaaa agcaaagagt tgcaatagca ggaattattg
    17041 ctatgaggcc taaatgtata atatttgatg aagcaacagc aatgttagat ccatctggaa
    17101 gaaaagaagt tatgaaaaca ataaaaagac ttaataagga agaaaatata actgttatac
    17161 atataacgca ctttatggaa gaagcagtag aagctgatag agttgtagtt atggaaaaag
    17221 gaaagaaaat cttagaggga actcctagag aagtattcag taaaataaag atgttaaagg
    17281 aaatcggttt agatgtacct tgtatgacag agttatctag tttattaata gaagaaggta
    17341 taaatattag tagtgatata ttaactgtgg atgagatggt gatggaatta tgtcaattat
    17401 agtaaagaat ttaacacata tatataatga agggatgcct tttgcaagta aggcacttga
    17461 tgatgtttct tttgagatta aagatagaga ttttgttggt cttataggtc atacaggctc
    17521 aggaaaatct acattaatac aacatttaaa tggattacta aagccttcct caggagaaat
    17581 atttataaat gattttaata taacagataa aaacttgaat ttaactgaga ttagaaaaag
    17641 agtaggggtt gtatttcaat atccagaata ccaattgttt gaagaaacga ttgataaaga
    17701 tatagctttt ggaccctcta atttaggctt agaggagtct gagatacata acagggtaaa
    17761 agcatcaatg gaagctgtag gacttgatta tgagggattt aaagacaaat ctccttttga
    17821 attatctggg ggacaaaagc gtagagtagc aattgctgga gttatagcca tgaacccaga
    17881 agttctcata ttggatgaac caactgctgg tcttgaccct ggtggaagag atgaaatttt
    17941 taacttaata aaagacttac atgaaaaaaa gaatatgact ataatactat catcacatag
    18001 tatggatgat atggcaaagt tagcaaaaac tttaattgtt atgaatcatg gtagcgtaga
    18061 atttatggga actcctagag aagtatttaa atcaaatgct agtaaattaa aagatatagg
    18121 attagatatt ccacaagttt tagagcttgc attgaaattg agagaaaaag gctttgacat
    18181 cagtgaggat atattgactt tagaagaagc aaaacaggaa atattaaaag ttgtgagagg
    18241 acgaggatta tgttaaaaga tataactata gggcaatact acccaacaag ttctgctatt
    18301 cataaattag atccaaggat aaagcttgta gcgacaattg tatttatggt atctatattt
    18361 gtagtaaata aattttggcc ttatatagta gttttattat gtttattagc tatgataaaa
    18421 ttggctaata taccagtcaa atatattgtt aagggtgtta aacctcttaa atggattatt
    18481 ttgtttacat ttcttataaa tatttttttc ttaccaggtg atgaaatatg gtcatttgga
    18541 tttttagcta ttacaaaaca gggtctaaga caagcaattt ttatggcaat aagattaata
    18601 tttttagtag ttggtacatc attgcttact ttaacaactt caccaataga attaactgat
    18661 ggaatagaga gattgttgaa tccatttaag aaaattggtt tgccagttca tgaacttgca
    18721 atgatgatga caattgcttt acgttttatt cctacattgt tagatgagac agacaaaata
    18781 atgaaggctc agatgtctag aggtgcagat tttgaaagta agaatcttat aaatagagca
    18841 aagaatttag taccactttt ggtaccttta tttgtaagtg catttagaag agctgatgaa
    18901 ttggctatgg ccatggaggc cagatgttac agaggtggac ataataggac taagatgaga
    18961 gagtctgtta tatcaagaag ggattatatg gcatgtgtat ttcaggttgt ttatctggga
    19021 gcaattatag ctactagatt tatagcaatt taattaataa gttatagata taatgaggta
    19081 aagtatgaga aatataaaaa ttaaaataca atacaatggt aagaattatt gtggatggca
    19141 aaagcaacct gattctttag ggatacaagg tacaattgaa agggctatat atgatataac
    19201 aaaagaagaa accagtctta tagggtctgg tagaactgat tcaggagttc atgctattgg
    19261 tcagattgca aattttaaaa ttaattcagg tataagtata gaaagtatac caatggcact
    19321 aaatgccaag ttgccaaaag acatatcagt tatagaagca tgtgaagtaa atgatgattt
    19381 tcactccaga tacagtgcaa aaggtaaaac atataaatat ttagtatata atagcaagtt
    19441 tagaaatcca atacttagtg agatatcata tcaagtaaaa tatgaacttg attttgataa
    19501 aatgtgttca gaagcaaaaa gtttattagg aacacatgat tttaaaggtt ttatgagttc
    19561 tggttcatct gtaaaagata cagttagaac gatatatgat attgatataa gtaaaaaaga
    19621 tgacttgatt acttttgaga ttagtggtaa tggtttttta tataacatgg tgaggattat
    19681 tgttggaaca cttgttgata tgggtcgtgg cagaattaac gaaccatttt tagatataat
    19741 acaatcaaaa actaggtcaa gatgtggaca tacagctcca gcacaaggat tatttctcaa
    19801 aaaagttcat tattaacttg acacaccagg ctacatgtat taaaatatat gagaatgtgt
    19861 taataagtcc acttgccccg gacttgacat aaacggtaaa aaaattttat gaaaagtagt
    19921 taaggaggga cagttatgaa aagttatata gctaagccag ctgatgtaca aagaaaatgg
    19981 tacttagttg atgctgaagg aaaaacatta ggtcgtttag caacagaaat tgcgacagta
    20041 ttgagaggta aacataaacc aacatttact cctcacgttg atggtggaga ttttgttgtt
    20101 gtagtaaatg cagaaaaaat agttttaagt ggtaagaaat tagatcaaaa atattacaga
    20161 taccatactg gttatgtagg tggattaaaa gaaatatctt acagagatat gatggataag
    20221 aaaccagaag aagttatatc acatgctgta agcggtatgt tacctaaaaa taaattaaga
    20281 agcagaatga tgactagatt aagagtattt gcaggtgctg agcacactca tgcagctcaa
    20341 aatccagaag ttttaaactt taaataagat taataggtga aaggaggagt ttataatggc
    20401 taacgttcaa tattatggaa ctggaagaag aaaaagttct gttgctagag taagactagt
    20461 tgcaggtgaa ggaaatatat tagtaaatgg aagagcatta gaaaattatt ttaattatga
    20521 gacattaata agagatgtta aacaaccatt agttttaact ggtaatgaaa ataaatatga
    20581 tgttatagta aaagttgaag gtggaggatt cactggacaa gctggagcta taagacatgg
    20641 aatatctaga gctttattaa aagctgattt agatttaaga cctgctttaa agaaagaagg
    20701 tttcttaact agagatgcaa gaatgaagga aagaaagaaa tacggattaa aagcagcaag
    20761 aagagctcca caattctcaa aaagataatt acttattttt ttggaaaagc aaaatccttt
    20821 atttactgtt attcagaaag tattggtatt ttaaaaatgt tgggttaagt taattattga
    20881 tatataataa aataataaga tatcttatta ggatgttttt cattaagaag aacatccttt
    20941 ttattttgac tatttaataa aaaacatatg ctaataacat ttaaatttta tttttataat
    21001 attgtaattt aaaagtttat ataaggttat atattttaag atattattta acaatataaa
    21061 ttctgaatat acgttatcaa aatttataat ataa
//
LOCUS       DAELJO010000053        20984 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELJO010000053 DAELJO010000000
VERSION     DAELJO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.053"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(89..778)
                     /locus_tag="KPB88_001672"
     CDS             complement(89..778)
                     /locus_tag="KPB88_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0053954.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(841..1689)
                     /locus_tag="KPB88_001673"
     CDS             complement(841..1689)
                     /locus_tag="KPB88_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0053955.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(1708..2595)
                     /locus_tag="KPB88_001674"
     CDS             complement(1708..2595)
                     /locus_tag="KPB88_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0053956.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(2653..3456)
                     /locus_tag="KPB88_001675"
     CDS             complement(2653..3456)
                     /locus_tag="KPB88_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053957.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(3616..4068)
                     /locus_tag="KPB88_001676"
     CDS             complement(3616..4068)
                     /locus_tag="KPB88_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG0053958.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(4198..5700)
                     /locus_tag="KPB88_001677"
     CDS             complement(4198..5700)
                     /locus_tag="KPB88_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0053959.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(5733..6599)
                     /locus_tag="KPB88_001678"
     CDS             complement(5733..6599)
                     /locus_tag="KPB88_001678"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG0053960.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(6684..7355)
                     /locus_tag="KPB88_001679"
     CDS             complement(6684..7355)
                     /locus_tag="KPB88_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG0053961.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(7628..8119)
                     /locus_tag="KPB88_001680"
     CDS             complement(7628..8119)
                     /locus_tag="KPB88_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0053962.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(8141..9505)
                     /locus_tag="KPB88_001681"
     CDS             complement(8141..9505)
                     /locus_tag="KPB88_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0053963.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            9924..11099
                     /locus_tag="KPB88_001682"
     CDS             9924..11099
                     /locus_tag="KPB88_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0053964.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(11272..12669)
                     /gene="asnS"
                     /locus_tag="KPB88_001683"
     CDS             complement(11272..12669)
                     /gene="asnS"
                     /locus_tag="KPB88_001683"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG0053965.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(12746..12979)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13148..13342)
                     /locus_tag="KPB88_001684"
     CDS             complement(13148..13342)
                     /locus_tag="KPB88_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG0053966.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(13632..15305)
                     /locus_tag="KPB88_001685"
     CDS             complement(13632..15305)
                     /locus_tag="KPB88_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0053967.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(15315..18713)
                     /locus_tag="KPB88_001686"
     CDS             complement(15315..18713)
                     /locus_tag="KPB88_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0053968.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRNINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(18881..20077)
                     /locus_tag="KPB88_001687"
     CDS             complement(18881..20077)
                     /locus_tag="KPB88_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0053969.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(20098..20919)
                     /locus_tag="KPB88_001688"
     CDS             complement(20098..20919)
                     /locus_tag="KPB88_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0053970.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDNISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         6766 a         3752 c         2224 g         8242 t
ORIGIN      
        1 tagacaataa aaaagaatct ataaatagat tctcttaata atatttttat ttaattaaaa
       61 ttctaataat actcttactt aatcataatt aattattttt aaaatacctt gataattcag
      121 tttctttaac ataaaaacct ttattgtcaa ttttaataag ttcttctttt tgcaatacct
      181 ttatcattct ggaaacagtc tcccttttac agccaagcat atcagctaaa tatgtaactg
      241 taatatttac atttagtaaa gtccattctc cacagcaaac tccaaattct ctacttaacc
      301 tatataattt tgctgcaagt tttttatcta cttttatgga tatagaattt ttcaactgtc
      361 tatataaacg tcttattctt ctttccatat aagaaataat atcctttgta agttgaaaat
      421 cattttgcat aatttctaaa aattcttcaa tactataata agctacaatt gaattttcaa
      481 atgcttcaca gccaacagta gagtttttag aagcgtcaag tgtaatatca tttatgactt
      541 ccccactatt taatatgaaa attacttttc tctctccatt ttcacttatc ttaaaaatag
      601 atacctttcc ttctaaaagt atataaacac tgtctaagct atctttttca taaaataaaa
      661 tttctttttt ttgtaatctt tttagtttaa ctttttcttt aagttgattc ttcgttttag
      721 tacttaaatt attaaaaatt attactttat caatattata atcttttaat ttagacatgt
      781 aatatctctc cattcctaaa tttttaagag agctctataa aacattacaa atttcttatt
      841 ttattcattt ttaaacaatt ttatagcatt tgtagtttta attttccttt caatagcttc
      901 attagaaagt tcttttacta cctgtgtata tactaaatca ataatgaaaa attgtgctaa
      961 tttagaagat attgaaccgg tttccagtac tgtttctccc gatacataag ataagtgtac
     1021 atctgaaata tcctttaatt cagaattttt attttcagta attgatataa ttttagcatt
     1081 attggctctt gcaagcttta cagtttttat aatttcttct gtttcaccag aatgtgaaat
     1141 tgcaattatt aaatctcctt cttccattat ggaagacatc attatcattg tatgactact
     1201 gtcaaaactt acacaattaa gacctattct cataaattta tagtttgaat cctgtgctat
     1261 tataccagaa tatccaatac ctatgaagta aactttcttt gcatttatta taagccttgc
     1321 gcagtcatgt acatccttac tattgattat ctctactgtg ttctctaaag tggttacatt
     1381 tgaacttaaa agtttttttg ctgtatccaa agctggctca tcattttgta tatttttatt
     1441 tataatattt tttttattga tagttgatat ttcttgtgct aaagtaactt taaaatcctg
     1501 taatccatta aaattcattt tttttacaaa cctagttata gttgcttcac caatattgct
     1561 ctctttagct atttgtgata ttggtttata aaatacatca tctatatttt cttttatata
     1621 agctattaat aatttatctg attttgtgac tttaaaagtt ggtgattcca attgttctaa
     1681 aatactcatt aaaaaccctt ctattcttta tattttgttt aaaaaattat ataatgctcc
     1741 aagcattcct gcatcatttt gtctctttgc aaattctata ttagtgtttt tgtaaactct
     1801 ctctataaga acctccttta atgctttgtc tatttttggt tttaggaact tttcttgtgc
     1861 cataatccca ccacctaaaa ttaccacttc tggatttatt acataacata tatttgcgat
     1921 tccaactgct aatgatttta tcatattatc gagttctttt aaacaatctt catcattatt
     1981 ctttgctaaa tcaaaaatta tttttccatt taagtcattt tcattcaaat tttttatttt
     2041 tgctactttt ttaattagac tgctagtaga tgccaattct tgaaaagaac ttccatttac
     2101 attcatatat cctacttcac cagcactatt actaaaccca tttatcagtt tattatttat
     2161 tataatacat ccaccaattc cagtacctat tgttaaacat atagaagatg ttgcacctct
     2221 acctgctcca agccacattt caccaagtcc agcacaatta acatcatttt caacttcaca
     2281 ttttatatta aactcttttt ctacttcttt ttttaattgc atgcccttat agtttggaat
     2341 taattcttct aatgcaaata atattttacc ttcaactggg tcaaccatac ctgcagttga
     2401 tatacatata ccacttattt cattttcctc tatatatttt tttactatat cttttatttt
     2461 ggctaatatt ccttctccac ctttttcctt tgcttcagta tcaagacttg atttatcaat
     2521 tataaatcca tcttctctaa taaaaccata ttttattgaa gtacctccaa catctataca
     2581 aacgtaattt ttcataacat atctccatac aaattatttt tataaattaa caactctatt
     2641 taaaatttta tattacacaa atcttttact tatcttttcc aattcacaga tatatttcct
     2701 tgcttctgat aagtctatag tatcacttaa tacacttttt tctttggtta tatgatactt
     2761 ataaatattt ttacataagt cctctcctaa catattaaga ccttcttgag tgtaatgaac
     2821 atcatcaata gttaatcctc tgtcatgaaa ttgcatagca attttacttc ctacacacag
     2881 tatatcacat tcattacaag cagattcttg agctgctgca attcgtcttg ttctaataaa
     2941 atattcatct tctccaagcc aataaccagt taaacttaca aacattttat caatcataca
     3001 ttcttcttta agcctatacg caattttttt tagactttct ttatagtaca atacatcact
     3061 accatatttt gcatcacttt caccttgaat ccaaataacg tatttatttt ctatattata
     3121 atgtttactt aaggtagtac agcctgtatt aaatttttct atagtgtcat ttagaaacca
     3181 gttattatca tgattccagt tttttattct tgaaccatct tttgcattgt gaataaaaca
     3241 aaccttattt ccagtaagct tattccattc atttgaaaat gcaggtgcta tagtaccatc
     3301 accttctgaa gtctctagct cacatctcat atttataatt tcatcagtac tcaaatattc
     3361 aaaacaattt tcatttggaa tgatgctctc ttttggattt cccaatcctc tagcattgct
     3421 ttgtcctatt aataagaata catctatatt ttccataagt taactccttt acaaaatttt
     3481 aagcttttaa ttattttcaa ttttctcata aaaaactaag gctgaaaata gtaatctaac
     3541 taaaatttac gaagtctcac taatcgctga aagttaaatc actattttca gccctagtgg
     3601 ttatataaat tatcattatc taaccaaaac cttaaaaata gcttttttga tattttcttt
     3661 ttctaaaact tttattcctg tcatatgagc atcttccgga taacaaacta aaaaatcacc
     3721 ttttctaagt tccatgtatc cattattttt accatcaaga ggcaagaaat cgcccttttc
     3781 ttcatatatt ttttgttcta aattttctat aaaatttata tctattcgtt cacatccatc
     3841 aagcatcaaa tgaacatcta tatacttctt atgagcttcc cagaatctat cttctttttc
     3901 acaagtttta tactctacaa tattcacaaa aatatcatca ccatctattg tataagtacc
     3961 tttttcataa tctattaatg aattatcttt agcatattca aaacatctaa gtaaatcttc
     4021 atgcaatgtt gaatatgtct tactatgatt tatatttcca aatatcataa gcactatctc
     4081 cctatataat tataaagtct atttttatac ttttatatac aaagtatatt atacaatctt
     4141 atagagtaac taaattattt atctaatcac tctataagac aatttatcat ttaaatatta
     4201 tttttcataa taaacagtag tctcagcttt tggtaaagat acttttgctt taactttagc
     4261 ttctattgca ctaactattg tacctaccac tacagaagtc gtgattgtta ttagagaata
     4321 tgcccaagaa gttacttctg gcatacggta ttttaaataa acaactaaaa ttgctgatac
     4381 tatgaatcca acatatgctc ccattttagt cgctttttta caaaatgcac caagtacaaa
     4441 tattcctgct aaaacaccta gtactagccc cataaaacta ttgaaccatt catatgctga
     4501 ctttatttct ccatttgcta atactattgc aacagctata gaaagtatac ctacacctaa
     4561 tgatatatat tgtgctatct tagtttgctt ttccatactt atatcttttg ttatgatagt
     4621 ttgtatatct aaagtccaac ttgtagcaac tgaattaagc cctgtcgata aagttgattg
     4681 agaagctgca tatatcgctg caagtagtat tcctgttata cccactggta attcataagc
     4741 tatatatgat gcaaatactt ggtcttgttg aactgtttga gctaaatctg ggttttgagt
     4801 atagtatata aataaacaag ttccaattag ataaaatact gttgcaagtc ccattgataa
     4861 aacaccattt ccatatgtca ttttattaag ttgctttata tcagttgttg tagtaaacct
     4921 ttgaactata tcttgactcg atacatatga tgaaaaagta ttaagaccag ctccaacaaa
     4981 tattataaat gcacttgtag atagtatatt tttatcaaac ataacttcat tacttgctaa
     5041 gaacttgtgt ccagttgtaa gagtatccat tactgttcca aaaccaccat ttatacttgc
     5101 tattaaatag aacaatgtcc ctgtaacacc tactattaat accattcctt gtataaaatc
     5161 ggtccacaag acagccttta atccacctgt atatgagtaa attattgcta ttacacccat
     5221 aactattatc agtacattta caggtattcc tgtaagacca gccagaacta ttgatggaag
     5281 atacattata atagacatac gtcctatttg atatattata aacatcaaag ctccaagtac
     5341 tctaagacct ttgctttcgt atctcatttc taagtattca tatgctgtat ctatttctaa
     5401 cctactatat agaggtaaga aaaattttat tgtcaaaggt attgctataa acatacctaa
     5461 ttgtgcaaac cacaaaatcc aagtaccacc ataagaattt cctgctaatg ataaaaatga
     5521 tattggactt aataatgtgg caaatattga tactgatgta acccaccacg gaatagttcc
     5581 atcacctttg aagaactctt ttcccttcat ttcctttttt gaaaataaca atcctgcaaa
     5641 taaaactgcc aataagtaaa ccactagtat tgcaaaatct atccaagtaa atcctaccat
     5701 ttgactaatc ctcccatact aaaagtattt tattaaaaat actttctgta tatttctttt
     5761 gctcttgatt tcatttcatc agttgcttct ttcataggtt gtctacagta tccagcctca
     5821 acaccttgtt cttcaagtag taatttaatt gtttgatata agccatttcc taatatatct
     5881 gttattaaat cattagtcac atgttgtact tcaagtgctt ctgatatttt ttcattctta
     5941 gttaattcga atatttgtct agctctgaca ccgtttacat tgaatgtaga accaattgcc
     6001 ccatctacac ctaaaactgt agctggtaac atcatttcat caaatcctgc aaatattaat
     6061 ttatttggga atgtttttct cattctctct aataagtaga aatctgctgc tgtaaactta
     6121 actcctatta ttttttcatt ttcaaataat tctccaaact ggtctaaact catatcaaca
     6181 ccagttaaaa atggtataga gtatattatt aatctattat ctacagaatt tattattgta
     6241 ttataatagt gttttatttc ttcaaaatca aatttatagt aaaaaggtgt aacagcactt
     6301 attgcatcat atcctaaatc agttgtaaat ttagcaagtt ctacagcttc ttttaaatta
     6361 actgaaccaa cttgagctat taattttatt tcttctttca cttcatcttt agctatttca
     6421 aatattcttt tcttttcatc agttgaaagc atgaagtttt cccctgtact tcctcctaca
     6481 taaagaccat ctactttaca aacatctata ttgtgtctaa ttatttgtct taaccctttt
     6541 tcatttatat ttccttcctt atcaaatgaa actaataatg ctgaataaat tcccttcata
     6601 tctgttgttc tcatatcttt acctccaatt ttaattttca aatatagtta aatataataa
     6661 ttttttaatt tataaattaa tttttaatta atttcaattg ctttaacaaa tttttctgtt
     6721 attaattgtg gtcttgttat tgctgaacct actactgatg aatgaacacc caactcaaag
     6781 acctttttta aatcttgagg agtatttacc tttccttcag ctattacagg tatttctaaa
     6841 tctttaacta atctttccat taatacaaaa tctggacctt ctaatgtttt agtatatgga
     6901 gtatatccag atagtgtagt tgaaacgcaa tctacaccat actcttgtgc tttgatagcc
     6961 tcatcataat ttgaaatatc tgccattact agaacaccgt tttcttttat atactttata
     7021 agttctttta agtcttcatt atttggtcta actctattgg tagcatctaa tgctatcatt
     7081 tcacaccctg ttgttaaaag ttcatcaact tcttttttag ttggcgttat atatatatct
     7141 gaatcttcat agtttctctt tataatacct ataacaggaa gtccagtaac tttttttatt
     7201 tcaataatat cttcaactcc ttgagctcta attccaacag ctccaccttc catagcagcc
     7261 ttagccattc tccccattat aaaaggacta tgaagaggtt cattttctaa agcttggcat
     7321 gatactatta atcttccctt taccttatct agcatttttt aaattacctc gtttctgaaa
     7381 tttattttct tgatttatgt attaataata acatgaaaac tttttctttg caatattttt
     7441 tcatattttt ttctcgcatc tatattttta ctgatttatt ttatatatta gcttgttttt
     7501 tacatttaaa agaaatttta tttcattttt ttggtgttta ttttcatttt atataactac
     7561 tgttataagt ccatttaaat aatttcaata aaaaaaccca tgtatactat acataggttt
     7621 cccaatatta ttctaaatcc attataattt ttcttccaca gcatggacaa aattcagcct
     7681 ctgtatctat ctcactttca caataaccac aaattataaa atcatcacat tcattttcca
     7741 attcaaattt tttaccacat tcagcacaat atttatcatt tacatttcca acataaccac
     7801 actcacaagt tacattactt tctgccattt ccatttcatt aatttccata tttttttcat
     7861 aaattaactt atcaagttct agtaactcat cacagaattt atcaaaacta ttatcattaa
     7921 tagaatcttc tctaattttt tgatatgtca gtattcccat ttccaagaat atctttgctt
     7981 tcttttcttg aatatcatca atctctctat ttaattttga tttttcttta tataattcaa
     8041 ctttaaattt tccactacca aactttttct taaccttaga aaatttatca tctactttat
     8101 tattagtatt tccttccaca gaaattccct ccaatcatat ttaatcagct gaatttagct
     8161 tatatcctat taaagatatc acaaaactat atacaaatga aattacaata gcttgtaaga
     8221 ctttaaattg cattattaaa gttgttccat aattagttgt attaaagaaa ttaatattac
     8281 tatctagtat aaatgtacta aataaagcca atattcccat caaaaatgca taatatatac
     8341 taaatactat tataggtctt atactatctg tattatactt aaatcttagc ttgtatccat
     8401 ttaataataa tattaacata gacaatgctc ccattgcata aaacattaat ttagtatctc
     8461 caaataaaga tgaagaactt aaagtaaaca ttgatactgt tgagtttatt atggttactg
     8521 aaattccatt tgcaaaagcc cacatataac tagctatttg tgggagtata gtaaataaat
     8581 ttaatccatt tgaataacta ctcatatcta gttcatttaa gtaactacta tcagatatag
     8641 ttagtaccaa tagtattact aatactaaaa cataacctaa tatgaatgtt cttatagaat
     8701 ttgaaaaaag agataaatac atattttctt tttcataact ttttttataa gtagttatgt
     8761 atgtacatat aaaccctaat acgaatccat ttaaaagaac actaaaaaat tcataactat
     8821 attctaatgc atatccatat tctaacatac tatgtgaact agtttttaca ttagtaaaaa
     8881 tagataaaat agctaaaact aatccataaa aaattccaac tcctagagaa tttgctaata
     8941 cagtttttga atctctacat ctttttctca taaaaattag gtttgatatt aataatacca
     9001 aaatagggat tataagtaat atcaacaaac ctatattggc atttataaac ccagaaccca
     9061 tcatggtaga catagataca ctaatttgac ccaagttgag agctaggatt atatgtgctg
     9121 gatttactaa ataactaata tcattaaatt gaatagttat aatagcttta aatatcaatg
     9181 caataataaa caatataaat atggttgtaa atgttgttaa aaatacacct cttttatttg
     9241 catgatatga aagctctttt attttagatt tcaagtctag ttttgtttca agtggtctta
     9301 atgatgctat ttcataaagt tcatttccac aaaatttaca gtaattatcc ttagtattat
     9361 tttctttatt gcaactagaa caaatttttt cttctttact tttaagagtg atatggctat
     9421 tttttatttt tctagttggt tcacctgtct ttttggaata attattatgt gaataattca
     9481 tattatttat tttcttttca cccatttttt ccctccaagt tttactattg taaattatca
     9541 aatataacta tatcatataa aacttaatta taccttaatt ggatatattt atctatttta
     9601 tttgtttatt gctgttgttt tttaacagtt cttgatattt aaatagtttg ttaatgcgaa
     9661 ctttattatt tatattttat tatgcttaat ttaaatttat tctattcttt attttcagta
     9721 gtaaactatc tgtaaatctt aattataata aagtattaat caataaagtt actcgaaatt
     9781 atctaataaa ttttatctat cacaatttta gattgttaca cacaatatat tttgatataa
     9841 ttaaatcata aacgaagtat tccaaatatc taatgataga aaggaatact tatttttatg
     9901 ctttattttt aatggaggct tatatgaact tagaaaaaat aaaaaaattt tttggatttc
     9961 ctctagacaa aaaacacaaa gacaatgttg agaaagaact ctgcaattat ctttatcata
    10021 cgggtaaaat catttgcata ataatagtaa tatctcaaat aattatgatg atatcaatct
    10081 cagctagaga aggaggccct tttgtcacac caagacgcca atggtatttt tatatgtatg
    10141 taacactatt ttgtgtatct tctgtcttta tgtttgtatt ttcttatctt tggaataaaa
    10201 aaaaggattg ctgtacgttt tatataaatt tatggataat ttatgtgact tttttctgcc
    10261 tatggggttg tgtaattact ctacttgacc agttaggtgg aaatgaccta agtgtattta
    10321 gttatattat gttagcagct gcagctttct cagtattaaa atttggagaa tcagtaatag
    10381 tttttggagg atgctttttg tttcttaatt tattcttacc tgggattcaa actataccag
    10441 gcaatgagtt tagtaatatt gtcaacacat tttttataac atcccttgcc atttttgttt
    10501 ctacaatgct atatagaaat cgtgtattta cttactatga taaactttta attagtcaac
    10561 aatatgaaga aatatctcat ataaataatt tacttaacga caaagttatg acagatgacc
    10621 ttactaaaat gaacaaccgt agataccttg aagatattct acaaaaaaaa ttaatcagtc
    10681 atgcaaatca agaacctata actggaatga tgatagatat agattatttt aaacaattta
    10741 atgataatta tggacatcaa aagggagact attgtctaca aaatattgcc aaagtaattt
    10801 tagattttac aagttctgaa aatgaacatg ctatacgata tggtggtgag gaatttttta
    10861 tatgtatttt taactgtaat gaggcagatg ctaaacttaa atcagaatgt ttacgaaaaa
    10921 ttatagagtc ctgcaatttt gagcgtgatg atgttccttt aaaatgtgta actgttagta
    10981 ttggagttta tacaaagaaa attggaaaga caataacatt aaatgagttt ataagtaatt
    11041 gtgataaggc tctatatact gcaaaagaac aaggacgtaa ttgtgttgca ttttattaaa
    11101 aggttacata ctattacata aacctataag tattatatcg caaaataaaa gtaatcaaat
    11161 tatatattta tagtacaata aaaataactt gcatatctct aaaatagata aatatgcaag
    11221 tcattttagt gtttatactt aaagttatat aagttttatt atatataatt attaaaattc
    11281 agcattttta ggagttcttg ggaaaggtat aacgtctctt atgttagaca tacctgtcat
    11341 atacataatt attctttcaa atccaagacc aaatccagag tgagtagcag taccaaactt
    11401 tctaagttct aaataccacc aataatcttc ttctcttaat cccatttcat taattctatc
    11461 tagcaataca tcttctcttt cttctctttg agaacctcct acgatttctc caactccagg
    11521 aactagtaaa tccattgctc taacagtttt accatcttca ttcattctca tatagaaagc
    11581 ttttatatct tttggataat ctgtaacaaa cactggacaa ttatatattt gctccgttat
    11641 atatctttca tgctcagttt gaaggtcaca tccccactca acaggatact caaattcatg
    11701 cccagattca attaataatt ccactgcttt agtatacgtt attctaataa actcagagtt
    11761 tactatctta tttaaccttt ctaacaatcc tttatctata aaactattaa aaaattccat
    11821 ttcttcagga gcattttcta atacatagct tataacatat tttatcatat cttctgcaag
    11881 ttccatgtta tcttcaaggt ctgcaaaaac tatttctggc tctatcatcc aaaattctga
    11941 agcatgtctt cctgtataag agttttcagc tctaaatgta ggtccaaaag tataaacgtt
    12001 tctaaaagaa agtgccatta tctcggcatt taattgacca ctaactgtta aattagcctc
    12061 ttttccaaag aaatcttgag tatagtctat tttgccatct tcagtttttg gaatatcatt
    12121 taaatccata gttgttattc taaacatctc tcctgcacct tcacagtcac tacctgttat
    12181 gattggagaa tgtgcatata caaagtttct ttcttggaag aatttgtgta ttgcataggc
    12241 agctattgat cttactctaa atacagctga aaaagtatta cttcttggtc ttaaatgtgc
    12301 tatcgttctt aaatattcaa gcgtatgtct cttcttttgt agtggatacg aactatctga
    12361 ttccccttct acaactatgc tttttgcatg tatttcgact ggttgtttag ccccttctgt
    12421 agatactaac tctccctcta caagtataga tgagcttatt gctaattttc ctacctcttt
    12481 aaaattttct actttctcat caaatactat ctgcatattt ttaaagaagc ttccatcatt
    12541 tagctctata aatccaaaat ttttagatac tctagatgtt cttatccatc cagctacttt
    12601 tacagtcttc cctatgtact gttctttttc tctgtacaat tcttttactg ttataaattc
    12661 cttttgcatt ttaaatcctc ctagtataat tgttattaat ttcttttaaa tataatttca
    12721 ataaaaaaag cccttcatcc ctatataaag ggacgaaaga ctattactct cgcggtacca
    12781 ccctagttgt cataatgacc tcttagttaa gcaatgctta aagcctataa cggtgccttc
    12841 cgtctaagtc tacttactaa ataagctttc gattagaaac tccaagatgt tcttcaatat
    12901 aaacctcgta ttgagctttc accatcctca actcgctaga actacatttt atatctactc
    12961 ttctcttcac agtatttatc ttttattatt tctgctaatt aacaaaatta taccaattta
    13021 taaattttta gtcaataaat ttatataatt gatactgatt gttatttaaa atataataat
    13081 tctaccttac ataatgataa taaatactta tcttcattac ttctttatta cttcaatttc
    13141 caaaatttta taatttactt ccattgtcaa cccagtcttt tgcttcttcg acacccttaa
    13201 ttgaaggtat tgaaacttta ctatgaggtt ttaatttttc aacatcagcc catgctgctg
    13261 tcgactcatt tccaactaaa agtgaacttt taagtctttt attatcttta ccttttaatg
    13321 ctcttgcctt tttactttcc aaaactatca cctctattat ttattttgta atagttttag
    13381 caattttaca aataaaattc ataagtctaa attataatga tttttaaatt aacatcatat
    13441 cttttcaata cttaatttat acctatttat atacaaaata tttactttgt gtattcaaaa
    13501 cataaaactg ttttttaatt ttcatattta ttttttatgc tattttattt tttatgctat
    13561 ttttctactc tatctaaagt atttgataca attaaactaa taatcaatta taattttaca
    13621 taggttctta tctatagagt atttgctatc tgttgaatcg tattttttaa atttaaaatc
    13681 ccatatccct gcgatacatt tggatatgta tatatctcaa gttttgtagc acccaatatt
    13741 aaatatgttt ttaaaacttg tgtaaacaat gataatctcg gtacattatc ttgtttttct
    13801 aagtattcca tcaataaggc taaaacacct gtgactatag aactgctcac tcctgttcct
    13861 gttcctgtat tatatgttcc atttttatat gtggatatta tatctacacc tgaagctact
    13921 atatctggct taattcctct tcctctaatt ggtcctttcg aagaaccaat ccacatacta
    13981 tcagtcttat tattaaatgt accaactgta attacatcat cactagcagc atacatagtt
    14041 atagtagcta ctgaatcagg gtctaaaaac cttgtgtctg gagctatcaa atttttattt
    14101 ggcaagtata tgtcatattc tccatttatt ataagttctg aagtcaatct taaaatccat
    14161 actccaggtt ttatatctct taatcttatc tccaaatttt cttttccaga ggtaatatat
    14221 ggataaataa atctcattgc ataagtagta ttttctaagt taaattttcc tctatatata
    14281 taaaagtctg gagaatacct tatatcatga ctaacttcac ctgaaggaga tattatctgt
    14341 gctcccactt tatctggacc attcgtattt agtgttatat caagagcata gtcatctcca
    14401 tcttgtatta ttacatcttg aacttctccc acactgctaa atctgccaga ataatgaata
    14461 tctgtattcc cttgatttcc tgcaccactc actactacca caccagcact actcaatata
    14521 ttaaaagtat ctagtattga agtagttgct acagcacttg acttaactcc aatagtcaaa
    14581 tttattatca gtggtttatt ttctgttctt gctatgttag taacatacgt tatagctgct
    14641 aaaaaatctg aaacactgta atttatcctt ccagcataat aagtatctgt atatgatttt
    14701 aatttaacta ctattaagtc tgattctgta gtaatacctc tgtactgaga atttatcctt
    14761 ccatttccaa ctaatatacc agaaactaaa gttccagtac ctatattatc ttgactcaaa
    14821 ctcccatcat ttctatttat ggctatattt aactcacttc ttgtaaattc acttccaaaa
    14881 ataaaacctt ctggaggtgg atttgtattt gcttcttggt cccataaata taaaacttta
    14941 ctagttccat catcatttat aaaatcagga tgcaaataat ctatcccaga atctataact
    15001 gctaataaaa ttcctctccc tgttatatca ttatatggat tttcataaat ataatcagtt
    15061 tcagctgctg ttgtaatggt ttctccatta tttacattat tagttatttc aattagactg
    15121 ctcattggtt cagattcttc ccaccaagca acttgtatta tattatttaa aatagtttca
    15181 tcaaaatcta aaggtacata tattactgca agctgactat ttaaaaccat atacctattt
    15241 attccatttt cttgtagggc agattctata tcaccttgat aaattataca ataagatttt
    15301 tccataaatc cctcctatct taaaacatca aacattcctc taacatccaa gagcccatat
    15361 ccagaatttg tatttggata tactgtgttt gaatcttttc tagcaccagc ttgcataaaa
    15421 gtttttattt tttgcacata tgcttggttt ggatatctac catcaacaaa agtatattga
    15481 aaatacattg cagcagcacc agctgcatgt gcacttgctg ctgctgtacc agttatagtt
    15541 gcatacgtgt tcccaggata agcagctatt atattaacac ctggtgctac tatatctggt
    15601 tttaacctgt cttcaatagt tggccctctt gatgaacttt gccataaact accattaata
    15661 gtattgtagg ctcctatggt tattaaatca tcttgaatcg caggataatt tatagtgtaa
    15721 aaagggtcta cctctctaaa tctagttcca ctttttaaaa gttctctatt tggaaggtat
    15781 aagttgtatc ttcctgttat aatataaact cctactaatc gaatcttcca tacacctctt
    15841 tttgcattct ttaaagtaac attagtaaat tgctgtccag aaaaagttgt tgggtatata
    15901 tatgttatag aatattcagt cccctctaaa tcaaataaac ctgttacttt attataattt
    15961 gaaattccaa cacttttact ttcttcacca gtgggagata ctattattac atcagcttta
    16021 tctggtctat ttaaccaaag ttctaatgat aattcttcct cgtcttcatt taactctaat
    16081 tcaacttcaa cagaacctcc aacatatggt attattccag atgtatgtgt ctgtgtattg
    16141 ccctcatttc ctgctcctgc cgttatacat aaccctctag taaagaacgc tttttcacta
    16201 ttactcctat tggttaaacc tgctaggcta ctggtaccta aagacatatt tataactaat
    16261 ggtcttccaa gttcaaaagc ttttttatat gcatactgag atgcagcaaa caacattgca
    16321 ctattataaa aatcatctat cttgccgagt tttattatta ttagctcaga atcttctgct
    16381 atacctgcat attcactatt tacatttcca agccctgcac atatcccact cagcatagtt
    16441 ccttgaccaa cttcatcctg agatagacta gggtcatttt ctgcgatagc tcgatttata
    16501 tcttctcgtg tatattctgt cccaatataa aaaccatcag gaggagttcc ttcttttgtt
    16561 tggtcccata aatatactat ttttgaagta ccatctggat aaataaaatc tgggtgtaga
    16621 tagtctattc ctgtatcagc aattgatatt agagtccctc gtcctgttat attaatatta
    16681 ggattatttt taaagaaatt tacgcccatt tcttctgttg caactacacc tccaaaagta
    16741 cctcgattaa tttctccaag taatgtcatc tttgtagtcg atacagttct tatgattgaa
    16801 ggaagttgca aaactcgttg tatgctatta tagtctgtat tatttataga tagtacacct
    16861 aatgaatcac ttatcttaaa aaacctttga ctcataccta acccagctag ttcttcttca
    16921 aattcaggag tatggactat atctattcct gatagtattc ctgattgtct acttgcattt
    16981 ttacttatat aaatttcatt ctctaagtca ttatgtattt gcatattata accatctata
    17041 aacttataac tattttcctc atctttaaat gacttattaa tatttctata tcccatatcc
    17101 tctgtttcta aatcttgatt tatattagat agagtgtaca aatctatatc tgataaattt
    17161 aaaaaaccaa atcctgatga attatttgga taagattgat tacttaatct ccttgctccc
    17221 tttagaagta atgcccttaa tttctgtgaa tacaaaaata aatcatttcc atttacaatt
    17281 ccccattcca taaacaatga acaaacccct gtaacatgag gtgtagccat gctagttcca
    17341 gttaatgctc cacttgtacc tcctggtaaa aaagatacta tatcttcacc tggagctaat
    17401 aaatcaggtt taaaaacacc taattgtgta tctccttcac cagaaaatat agatactata
    17461 tctgttcttg aattaaaact acctactgtt ataactctac ttgctgttcc aggaactgtt
    17521 acagtaagct cttgagtagg aatcagaaat ctagtatttc tatttagacc ttcagaagtt
    17581 ggcaaatata tatttacatt accagttact atatctattg gttcaaatac aattttccat
    17641 aacccaggag ttatctgtgt atttgaactt aattgtagag taactcttct tgtgagagaa
    17701 tacggtgcta tagggtaaaa ataaccagtt attctagttt cacccaaagt attccttatc
    17761 tcacctgaag ttagagagat tgcttgtgtt tgattatttg atgggtttac caaatgtaca
    17821 ctaaaatcat caacaaaatc tggccatata tttatgttta atatacgctc tccttcccca
    17881 actataaact caacttcttc tgtaatatta ttttgaagcc taatcctttt atgacctcct
    17941 ttatctgcat tatttccagc tgctacaact atattgtttt tccaaaataa acacatatca
    18001 tctatatatt gttcaaataa agatgttcct ctatgtgaac cttcattact tccataactt
    18061 atatttaatg taacaggcat tcttaattct aatgccctat ctaaaataaa tttaattgcc
    18121 ctcataaact ctgtacttct cgaaaaaata tcggtctgta tatttccaac tctaaccact
    18181 attattctgg catcacttgc aatagttgca caaatacctg caacatgtgt cccatgtaag
    18241 ctagttgttg aaataggtat atacatactt ccatctattg cattatttat atcttcatta
    18301 gtatataatg ttccttctct aaaaccttct ggtggatttc cttgaataga ttggtcccaa
    18361 taatataata tcttagacct tccatcacta tctctaaaca caggtagagt ataatctatt
    18421 cccgaatcaa ttataccaat tatagttcct tttccagtta aaccagttct atttttaaat
    18481 cctgttattc ctgtacttga aaaactttgc acatcctgag tctgtaatat aaaaggcttt
    18541 tctataaatt caatttctgg ataagttaaa agtatattta catcttcttc attacttgat
    18601 gttattattg catatgatga attaagtatc tctactgata cacctaattc ttcttctaac
    18661 ctcaatatat ctccattgta ttttacaatt aattcataat ttattataat cacctcgact
    18721 tctaaaatta ttattaatgt atatgttttg tgtgagtcta aaatgtatta gactatataa
    18781 ttttttaagt ttcagttata caatttataa tagatatatt aaaaaaaata aataatgatg
    18841 gtacaatttt gaataaatca taccatcatt tatatttact ttatcttgaa accattaaat
    18901 ttttcaatgc tttctcaaga ccttttacag tcaatggaaa catattaaat tcactttgta
    18961 tcattctttg agtttgatac caataattaa ttgaatccca tccatctcta agttctggat
    19021 tcatccatac aattttactg tactttctct taaacctttt aagccactct ataccaggtg
    19081 tgtaattata tggaccacga tagttaccac caatatgaag caactcagaa ggcgacatag
    19141 aagcatctcc aacaattata actttatagt ttttatctat attttttaat atccattcag
    19201 tattaataga tttacttaac caacattcag gtgttgtaaa taatctatca taaatacagt
    19261 tatggaaata atatatcttt acatctttaa aatgatttga tttacttaca gactgaaata
    19321 atgtattaca taaagtgcta taccctctca ttgaaccacc tgaatccata agcaataaaa
    19381 gcttcactgt attttttctt ggcttctcaa aaaataactt taaatatcct gcattattac
    19441 aagtctcttc aatagtctta tccaaatcaa gctctgtctt tggagcatct attctagtag
    19501 aaaattgacg aagtctcttt agtgcaactt gaaattgacg catatttaaa acttcatcat
    19561 ctctaaaatc tctgtatttt ctttctcctg caacttcaag aacagctcta ccataagtag
    19621 aatttccacc tatacgtata ccagtttgcc cttttcctga atgtcctaac tctgaagtgc
    19681 ctcctgtgcc tatccaataa ttaccaccat tatgaggaga atcttgttct tttattcgtt
    19741 cctccaattt agctcttaat ttatcaagat ttatatttgg attttgttct aatctttcaa
    19801 actcttctaa atctatatct ggttttttaa gccaattcat aatctcttct ggaatttctt
    19861 cttggtgttc tataccttta aaatactcca aaaacactgc atcaaactta tcaaaatcac
    19921 tttcactttt tattaaaatc atcctgcaaa gatagtaaaa atttgaaaag tttgaatagc
    19981 acagtccttt atccaatgct tcaattaaag tcatccattc atttaaagat acatttaatc
    20041 ctcttgacct caataaataa aaaaattcaa taaacataat tatccagctc ctattaacta
    20101 taagtgtctt tgcatagatt caatatcctc attattctta agtagtattc ctgcaaaagg
    20161 aacttctttt tctatttttt caataggcat tccactcaaa gttaatgctt gtatccagtc
    20221 tattaactct gatgtactag gctttttttg tatgtccttt aaacttctaa tccaataaaa
    20281 agtagtcatt acttgttcca ataggtgttc ttctacttta tcaaaatgta ctttaactat
    20341 ttcttccatc atgtctctat caggaaattc tatataatga aatatgcatc ttcttaaaaa
    20401 agcatctgga agttcttttt ccgcatttga agttataata actattggtc tttgtttagc
    20461 tcttacagtt tcttttgtct catttatgta aaactccatt ttatccaatt cccaaagcaa
    20521 gtcatttgga aattctaaat ccgccttatc tatttcatct attaaaagaa ttacttgttg
    20581 atttgagcta aaggcctctc ctagtttacc atattttatg tacttgctaa tattatctac
    20641 accttctcct ccaaattggc tatcataaag tctttgaaca gtatcatata cataaagccc
    20701 ttcttgtgct tttgtagttg attttatatt ccaaattact aaatcttttt ttaattcatt
    20761 tgaaattgct tgtgctagca tagttttacc tgttccaggt tcgcccttaa tcaaaagtgg
    20821 cttttcaaga gcaatagcaa cattaactga tgccatcaat tctggtgata ctacataatc
    20881 actacttcct ctaaagttag atatctcttt ttttatcata aatcctactc cttttaaaca
    20941 gtattttcta aatgtatata gactttatta ctatttttga tata
//
LOCUS       DAELJO010000054        20778 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELJO010000054 DAELJO010000000
VERSION     DAELJO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.054"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(14..856)
                     /locus_tag="KPB88_001689"
     CDS             complement(14..856)
                     /locus_tag="KPB88_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0053971.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVNYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1117..3159)
                     /locus_tag="KPB88_001690"
     CDS             complement(1117..3159)
                     /locus_tag="KPB88_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG0053972.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3311..4282)
                     /locus_tag="KPB88_001691"
     CDS             complement(3311..4282)
                     /locus_tag="KPB88_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG0053973.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKIQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4283..5260)
                     /locus_tag="KPB88_001692"
     CDS             complement(4283..5260)
                     /locus_tag="KPB88_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0053974.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5479..7038
                     /locus_tag="KPB88_001693"
     CDS             5479..7038
                     /locus_tag="KPB88_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0053975.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7144..8103
                     /locus_tag="KPB88_001694"
     CDS             7144..8103
                     /locus_tag="KPB88_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053976.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8106..8918
                     /locus_tag="KPB88_001695"
     CDS             8106..8918
                     /locus_tag="KPB88_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0053977.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9134..10036)
                     /locus_tag="KPB88_001696"
     CDS             complement(9134..10036)
                     /locus_tag="KPB88_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0053978.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10391..11569
                     /locus_tag="KPB88_001697"
     CDS             10391..11569
                     /locus_tag="KPB88_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0053979.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLSMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVGPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKDELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11578..12234
                     /locus_tag="KPB88_001698"
     CDS             11578..12234
                     /locus_tag="KPB88_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG0053980.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12234..12899
                     /locus_tag="KPB88_001699"
     CDS             12234..12899
                     /locus_tag="KPB88_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG0053981.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            13004..13780
                     /locus_tag="KPB88_001700"
     CDS             13004..13780
                     /locus_tag="KPB88_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0053982.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13887..15221
                     /locus_tag="KPB88_001701"
     CDS             13887..15221
                     /locus_tag="KPB88_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0053983.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15717..16628
                     /locus_tag="KPB88_001702"
     CDS             15717..16628
                     /locus_tag="KPB88_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG0053984.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPEDNNNSGSTDKPDNNKPESNKPEDNNNSDS
                     TNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMI
                     DNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIM
                     NPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(17020..20559)
                     /gene="nifJ"
                     /locus_tag="KPB88_001703"
     CDS             complement(17020..20559)
                     /gene="nifJ"
                     /locus_tag="KPB88_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG0053985.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7174 a         3259 c         2930 g         7415 t
ORIGIN      
        1 aaactactct ttattataaa ttcagtacag atataactct tttgatatgt aatgttaaat
       61 aaagtagttc atcttgactt acatagtaat cataattctt ttctacatga gtttttattt
      121 tatatgcaca atcataatcc ttttcataat ttactttaat tatatctata atttcattat
      181 ttgtatcatt gattttttct ttttttacta atcgttttgc aaagaatttt aaatgagtca
      241 gaagcctatc ataatttata tcatcttctt taaattcaac tctataataa tatcttataa
      301 tatttagtat gtcttttaca attttagtaa ttaatagtga ttcctttgaa cttcccttat
      361 agtttgaatt tactatatgc attgctataa aaccagcttc atctactgga aatttcacat
      421 caaattcttt atttatataa tctacagccc atttcccaat ctcatattct tttttatgta
      481 ttctttttat ctcatgtagt aaatcattct ttatagttat gttttcatga tatcttttaa
      541 ttgcaaatgc aatatggtca gtaagtgcta cataaatata ttcataaagt ttagtattca
      601 aattctcctc tgcataagca attatttcag tactaacctc aaaaaatttc tcatcaacct
      661 ggcttgctag tttactaatt ttatctaaaa agctttcatc ttgtattaca aaagttcttt
      721 caattttact tttatctatt tcttgacctt tctttttttg aaacccaatt ccacatcctg
      781 ttaatattaa ttcttttttt atattttcat ctaaaactaa tacaacatta ttattataaa
      841 tttgttgaat aatcataatc aatatctctc ccaaaataca atttccctta ttcataaaaa
      901 aatacctaaa ttaagataca attcacctgt ataccttaac ctaggtattg cctgcttacc
      961 agtaacaatc cagcttgtta acgcttatat attgtattca atttatggtt ttattctatt
     1021 ataaataaat atatatgtca agtaaattat tgaaaactat ttcctatttt ttagtattaa
     1081 ttttaacatc aaaaatcaaa ttttattcca ttatatttat gattctatca tcatcatttc
     1141 atcatagaga atattttcca ataactcttt agccacttct cttgatattt gcttagtttc
     1201 aactaattgt tgaacactgt ctctttgact ttgtatcgcc attactctgt agtattctac
     1261 tcttgcctca aacttatcat cattttgaat ctttctagta atccaattca aataacgatt
     1321 atagaatctg attgctatta aggcctcatg tctgttatct tcatcagtat ttttatttat
     1381 aaatgatata gcagttttag tagaaagtgc atatgcatgg tgaaactctt tgattatttc
     1441 tttattctca gatactattt ttgtaatttt ttcttttact tctttcttta cttttctatt
     1501 tataatccac atgcttattt ttaattttac aattttaaat atagattttt gaacagattt
     1561 tcttgatttt gtaatataaa atctatacaa tttaaaggct ctatcagaaa ttttaccatc
     1621 ttctaatagt ttcaaaacag ctaataatcc ctgttggtct gcttctgcaa tcatcatact
     1681 tataagttta ttatctggag tatttagtat tcctttttct gagtctacca actgttcatt
     1741 taaatcatgt ataactggtt gtacttcttg tgctttttca ttactaagtt ttaaaatggc
     1801 ttctttcaaa attagtttat atgcctcttt atcagataat aaaggttctg actcttgtac
     1861 gttatttttt aataatcttg gcagtaaaac tgttgccaat actaaactta taagaataac
     1921 ttctgatgat ataaataaaa cagtatctct cattggaaat acatctttat cagccaaata
     1981 aaaaggtata gataaggcag ttgctagtgt aaatgtacca tgaacaccac aagtagcaac
     2041 tattagtgag tatttacatt tagaaatact ctcttttaca acttcttcat ccttaaaacc
     2101 taaaaaacct acaaaaaaat tatttaaagg attcttttta ggttttataa aagaattatg
     2161 ccataaatat acccatataa atcttataac aaataaaata agtacaatta aagtagaaat
     2221 aaaagtcaat tccaatgcca tattttcatt tttactacta agtcctgtat atatacttgg
     2281 taacaaaaaa ccaagcagtg taaagacaaa tccattcaat acatactcca aaacatacca
     2341 agtattgtct gatattatcc ttaatttagt agttgtattt tgtaaatgct ctttttcaat
     2401 tccatgagct atccctgcca ttacaacagc taggattcca gatactccta tttcttctgc
     2461 tacaaaatat acaaaaagtg gagtcattat ttgtataact attaccattg gaatttcttc
     2521 caaattccac ttgtgtaaac taagtcttat ttttataatt atgtatccta aaatacttcc
     2581 tactattatt cctccaaaag ctgtaatcat aaattgaatc cctgcctctt ctattgaaaa
     2641 aactcctgta actgtggcaa gtactgcaac cttaaatgct actactccag cagcatcatt
     2701 taataaagat tcaccttcaa gtatactcat catattttta ggaaaattta atccttgtgt
     2761 aattgatttt actgctacta aatcagttgg agatattact gctgccaaag caaatgaaac
     2821 agataatggc atcccaggaa taagaaaatg tataaagatt ccacctgcaa acacggttat
     2881 taaaactaaa ccaaatgcaa gaagcaaaat tggttttctg agctctaata gctcttttct
     2941 cgatacattt tgtccttcac tgaatagtaa tggagctatg atacatatta caaatatttc
     3001 tggattaaat gaaaatgaga atttaaatgg caaaatagac aatataatcc ctagtacaat
     3061 ttgatatatt gcaagaggaa tattactcca ctttttgcta aatacagttg aaatcgttgt
     3121 acaaaccaat aatcccaaca atgttactaa aattgacata actacctctc ttttcaataa
     3181 taatattata gattaaactt atatatcaac taacttaata ttaacagcat gaaatcccat
     3241 cttaatcaaa aaaattttca gtttattcta ttattcttat tatatacatc aaatttaatt
     3301 tttgaaatat ctatactaga tgacatgcaa caaaatgatt tcttgatatt tcttttaaaa
     3361 ctggaatctc ttttttgcat atttccttac aataagggca tcttgtatga aacttacatc
     3421 cacttggtgg atttgttgga gatggtatgt ctccttttaa tataattcta tttttcttaa
     3481 ttgtagggtc ttgtattggt actgcgctca taagcgcttt tgtatatgga tgttgagaat
     3541 gtaagtaaag ttctccttta gttgcaattt ctattataga acctagatac ataacacaaa
     3601 ctctatgtgc aatatgattt actacactca aatcatgaga tataaataaa tatgaaagtt
     3661 tatatttatc ctgtaaatcc ataagtaaat ttataatttg agattgaata gaaacatcta
     3721 gtgcagaaac aggttcatca caaactataa attctggatt taatgctaat gctcttgcta
     3781 tacctattct ctgtctttga cctccagaaa actcacctgg ttttctattt aaatggtact
     3841 tagaaagacc acaactttct acaacattta atatattatc ttttatttca ttctttggca
     3901 ctaaattatg ttctataact gcctcggata ttatttgttc cacatttaat cttggattta
     3961 atgaactata tgggtcttga aatatcattt gtattttggg tcttaacttt ctaagttctt
     4021 ccttctctaa cttatatata tctacaccat taaattcaac tttaccttca gttttttcaa
     4081 ttaatctaag aatggttctt cctgcagttg attttccaca tcccgattct ccaactatag
     4141 ccaaagtttc tccttttttt aaatcaaaag aaattccatc tactgccttt acacatttaa
     4201 tttccttatc aaataatcca gatttaactt caaagtactt tttaagacct tctactttta
     4261 ataaactctc ttcttttttc aattatatct cctccttttt atataaccaa catgcaatac
     4321 tatgaccact attcaattct attaaaggag gtatctgagt tctacattta tcacatactt
     4381 tttcacatct atcactaaaa taacatgaat ctggcattcc aattggattt ggtacttgtc
     4441 ctggaatcga atacaatctt ttatctttgt tttttccaag agcaggcttt gattttagaa
     4501 gtccaatagt ataaggatgt tttggatttt taaataattc aacaacctct gccacttcta
     4561 taattttacc agcatacatc actaatactt tgtcacacat ttctgcaact actcccaaat
     4621 catgtgtaat catcattata gatgtgttta atttttcttt tatatttttc attatatcta
     4681 aaatctgagc ttgaatagta acatctaaag ctgttgttgg ttcatcagct ataagtaatt
     4741 ttggattaca tgataaagcc atagctatca ttattcgttg tctcatacca cctgataatt
     4801 catgtggata tgaattatat atttcttcag ctcttggtat ttctaccagt tttatcatat
     4861 cgattacctt tttcttagct tcttcctttg ataaatcttg atgtatcata ataccttcaa
     4921 tgatttgact tccaacagta aacgctgggt ttaaagatgt cattggttct tgaaaaatca
     4981 ttgatatctc attccctctt attcccataa gttcatcttc attcatcttt aaaatgtctt
     5041 tgcctcgaaa gattatttct ccaccttcta tttttgatgt tggaagtaac ttcatcaaag
     5101 acatggctgt tacagactta ccacatcctg attctccaac tattccaata gtttcacctt
     5161 catttatatt aaaacttact tcatttacac tctttacaat ccctttatct gtatgaaaat
     5221 aaacttttaa atctttaacc tcaattagtg ctttactcat tattttctcc tctcagcaaa
     5281 attacttaag ataacataca ccttttgatt gtattttata aaaatttaaa ttacaacatt
     5341 ttcttacatt ttattatttt tttctattaa ttataataat ataatagtta atttctatat
     5401 atattgattt aataatgatt actattatac aataaaaaat atgtgttaaa tattatttta
     5461 tggaggtttt ataagaatat gaaatttaaa aaattggcat cattaatctt agtttcatca
     5521 ttaatgctta catttacagc ctgtgcttcc aatgataaag acaagtctag tggaagcaaa
     5581 tctggtggaa ctgttgtaat acctatggca agtgacccag atataatcaa tggagctttt
     5641 gcaaacgtta aagaagactc tcttgcatca aatatagttt atgctcctct ttatacttat
     5701 gaaaaaggaa atttagtaaa ttatttagca gaaaaagtag attttaaaga tagtaaggaa
     5761 ttaactataa aattaaaatc caatttaaaa tggcatgatg gaaagccaat aactgctgaa
     5821 gatgtcttat ttacatttaa tacagtttta gacgaaaaac aaaattcacc atcaagacaa
     5881 tacttattgg tgggtgagaa accagttaaa gtagaaaaaa ttgatgattt aacagttaag
     5941 attactttac caactgcaag tgaatctttc ttatatggta tatctaaaat ttctccaata
     6001 ccaaaacatg tgtttgaagg agaatcaaat atagctaagt cagaaaaaaa taataatcca
     6061 gttggttctg gtgcattcaa gtttaaggaa tggaaaaaag gtgaaagcat agtatttgaa
     6121 aagaatgcag attattttgg tggagaacct aaggcagatt ctatagcgct aaaaataatt
     6181 cctaatgaag catctcaaga agcagcttta aataatggcg aaatttcact tatgaagaca
     6241 tctgctgaag gatatgaaaa ggcaaaatca aactcaaact tacaaactta tacttatagt
     6301 gaagaaagat taaactacat tgtatttaac caaaatatat caaacatggc taacaaagaa
     6361 gtaagacaag ccttatcata tgccttaaat agaaatgaaa tgatagaatc agcatatgga
     6421 aaagaaggtt ctgtacctgc taaatctata ttagttccag aagcagactt ctatacagaa
     6481 gaaggtgttg aaggatatga ccaagataca aataaggcta aagacttatt ggataaaagt
     6541 ggtgttaaaa ttgataaatt aaaaataggt tataacacag gtagatttgg gcataaaaac
     6601 tatgccttag tagctcaaca agagttgaaa aaaattggta tagaagctga aatagttcct
     6661 tatgaaagta aggcattctt caatatacta tttagtaata gcactgaatg tgatatgtat
     6721 gtaaatggtt atgcttgggg attagaacca aacccatata gaggtatgtt tgaaactggt
     6781 caatattgca accaaactaa atattctaat gctgaaatag atgctttatg ggaaaaagga
     6841 tttactgagt taaacaagga aaaacgtgaa gaaatatata agcaaataca acaggatata
     6901 tctaaggatg cgcctattta cactatagat tatgaacaaa atttaatggc agctcaaaaa
     6961 aatctaaaag gaataaaaga tgctaaacca tctcctgcaa tattatttga agattggtct
     7021 aaactgtatg tagaataatt gatgtaaaat tttatattat taattagaaa aggagatgtc
     7081 taaaaataat attgttttat tattttaaaa cgagacatct cttttaatta ggagaattta
     7141 cttatgttaa aattaatatt gaatagagta aaagttttaa tacctatgtt aatcttaata
     7201 tctatattat catttggact tttagaatta gctcctggtg acccagcaga tgcatatata
     7261 aatcctttaa tgtcttcaca ggatattgaa aatataaggg ttaatatggg gcttgataaa
     7321 ccagtatata taagatattt aaactggttg aaaaacactt taagtggaaa cttaggtatt
     7381 tcttatataa accatatgcc cgttacagaa caaattatgg aaaaaatggg aaatacattt
     7441 attcttatgg gtacttcttt gatattttca atattggtag ctatacctct tggaatattt
     7501 ttagctgtaa ataaaaattc aataactagt aaagtatcat ctgtttttaa ttatataggt
     7561 gtttctatac cttctttttg gataggtatg atactcattt caattttttc agttaaactt
     7621 aacattttcc catcaggagg tatgcatact attggaaatg atagtattgg agatttagta
     7681 aaacatttag ttttgcctgt aataacttta ggtctatata atactgctat ttttacaaat
     7741 tatgtagaag ctagtgtaaa tgaacaatta aaaaaacaat atgttattac agcaagagca
     7801 aaaggattat cagaaaaagt catactattt aaacatgtct tgaaaaattc actgacatca
     7861 ttggttacaa tcctaggcat gagtatacaa aaattagtca ctggtgcatt tgttacagaa
     7921 gtagttttct cttggccagg tatgggaaga ttaatgattg attcaatttt ttcaagagat
     7981 tatactgtaa ttatggcaat aactatgtta tcagcactat ttttaatttt aggtaattta
     8041 gttgctgata tactatactt attaattgac cctaaaataa aatctagcaa aggaggattc
     8101 tagttgtgtt tacaagtatc ttaaaaaaat taaaaaaaga taaatccttt gcttttgcat
     8161 gtattactct tagcataata atcatattaa ttataacttc ttttatatta aatatagact
     8221 ctaatgcaat tgatactact tctaaacttc aagaacctag cttaaagcat atctttggta
     8281 cagacgaact tggtagagat tacttctcta gagctcttta tggtggaagg atttcactta
     8341 gtgttggtat tttatcaatg cttatttctg tagtatttgg tactgctgta ggtgttatta
     8401 gtggatatat tggaggtaag ctagaccaat tcttgatgag attagtggat atgcttatga
     8461 gtataccaag tttcttagtt ttgattgtat taaatactta tctaacacct aaagtttcca
     8521 ctattatact aattattgga ttcttaagtt ggatggaagt ttcaagaatt gtacgtggcg
     8581 aaacattaaa aataaaggaa aatgaatact gtctagctgc taaagctact ggtatcaaaa
     8641 ctagaaatat actattaaga catatagttt taaacttaaa agagactatt gtagttgctg
     8701 gttcgttaaa tattgctaat gctatcttaa ctgaatctgc tcttagtttt ttaggtcttg
     8761 gaatccaatt gcctcttgca tcatggggta atatgcttca agatgctcaa agacatattt
     8821 ttgacaagtt atatttagct gtatttccag gattacttat atttttaaca gtcatgagtt
     8881 tgactataat aagcaaaaaa ttttctagta atacttaaca aattttatat agaaaataaa
     8941 aacagtgatt atctaaagat atacactgtt tttatttttc gtttaaaaaa atacatattt
     9001 tgtatattat aactttaaat actactctac catgttatgt aaagcgtatt attgctctca
     9061 aacatataga tgaaaaacta atagtttatt ataataaaaa ttgatttatt tatttgttat
     9121 tttaataaca tttttaagag ttctttttac taaagaaatc tataataaat ttaaatacat
     9181 gttggtctac atggctataa cgttcttttt taggtctaca taatataact ttccatgtaa
     9241 taggatgatt aaaaggcttc tctataattt tgctattttc aaaaaaatca tgaactggtg
     9301 atggtagaat tgttataaaa tcagaatttg tggttgatag caacaaaaaa tcccatgaac
     9361 cagacattat atatcttttt aacttaacat tttcatcagt aaatttttgc attagcttat
     9421 gatgtatcat aaaagtagga ttaaatattg caagtaactg ctcattcaaa tcgttccaat
     9481 ctatcttatc cttcttagca agtggatgat ttatatttaa aaatgctgta agttcatctt
     9541 cttgaagcac atgttcagtt actacacttt tatcaatttt atgtggatgt aacaatactg
     9601 cataatctaa ttcctgtaag ataagcattc tatttaagtc atatgcaccc ttctctacta
     9661 tatcaaattc tatatctgga ttatttgcaa ctaactgtgc aactacatct gaaaatgcta
     9721 ttccaagtat aagtggaggt attcctattc taacttttcc tttaaattga actgaatctt
     9781 ctcttaaatc ttccatcata tttttgtatt catttatgat atgttctgca tttacataaa
     9841 atctttctcc tgctggtgtt aacccattta acctgccttt atacctttca aataaatata
     9901 catcttcact ttcttcaaaa ttttttataa tttgacttaa tgctggttga gatatgtgaa
     9961 gtgtcttaga tgctattgat aaattaaaac cagatttcac tatttcaaca aaatatttca
    10021 actgttttat atccatttta aacccccttc cccaaaagta taatttagat tatatttttt
    10081 cttctaatga tattttatag gaaaatcaac ataatttcat taatatttta acaaaatatt
    10141 tttctaaatc tactataaat ttttatccta taaatttttc ttatacacta tataaaaaat
    10201 tagacttaga caaaacgctt ttttcgccgt aatctataat tagaaacagg aaaacctaaa
    10261 gcattaggaa taaaagtaat tgaaatactt aaaaacaagc ttgtattaaa aaagtacttt
    10321 attatagtgt tccctaattt ctttattcag aaaattttta attatcattc aagaaaggta
    10381 actattagat atgaaagatg tagtaatcgt atcagcagtc agaacaccta taggaagttt
    10441 tggaggtgta tttaaaaata cttcagctgt tcaacttgga actatcgctg ttaaagaagc
    10501 gatatcacgt gtaggactta atttatctga aatagatgaa gtaataattg gaaatgttct
    10561 tcaaacaggg cttggacaga atgttgctag acaaattgct ataaatgcag gtataccaaa
    10621 ttctgttcca agctacacag taaataaatt atgtggttca gggcttaaaa gtgtccagct
    10681 agctgcccaa tcaatcacat ctggtgaaaa tgatgtggta attgcaggtg gtactgaaaa
    10741 tatgagccaa gctccttata tcgttccaac tgctcgcttt ggtagtaaaa tgggtgacat
    10801 aaccatggta gatagtatgc taacagatgg ccttattgat gctttcaatc agtatcatat
    10861 gggaataact gcagaaaata ttgcaactaa atttgaattt actagagaaa tgcaagataa
    10921 attggcatta gaaagtcaaa ataaggctga gaatgctata aaaaacaatc gctttaaaga
    10981 agaaatcgtt ccagtagatg ttttaatacg tcgaggaaaa atagaaacta ttgataaaga
    11041 tgaatatcct aaattaagta tgacatttga aggtttagct aaacttaaac ctgcattcaa
    11101 aaaagatggc actgtaacag ctggaaatgc ctcaggtatt aatgatggtg cagctatgtt
    11161 aatactaatg agccaacaaa aagcagacga actaggtatt agaccacttg ctaaaataaa
    11221 atcctatgct tcagctggtg ttggacctga agtcatgggt actggtccaa tccctgcaac
    11281 tagaaaagca ctgaaaaaag ctgggcttag catcaatgat atagacttaa tagaagcaaa
    11341 tgaagcgttt gctgcccaag cattagctgt taaagatgaa cttcaaatag attcatcaaa
    11401 gttaaatgtt aatggtggtg caatagcatt aggacatcca ataggagcaa gtggagctag
    11461 aatacttgtt accttaattt atgaaatgca aaaacgtaaa gttgaaactg gacttgcaac
    11521 actttgtatt ggtggtggtc aaggaatatc aatggtggtt tcacgatagg aggaaatatg
    11581 aataaaatag ttagcattga tgaagctcta tctcatgtaa aagatggaga tacaataatg
    11641 gttggtggat tcatggccaa tggttcacca gaaaatttaa ttgattattt atgtagtaaa
    11701 aatataaaag atttgacact aatttgcaat gatactggct ttattgacaa aggagttggc
    11761 aaaatggttg taaataagca atttaaaaag ataattgctt ctcatgtagg attaaataaa
    11821 gaaactggaa gacaaatgaa caacaatgaa acagatgttg agttagttcc tcagggaact
    11881 cttgctgaac aaatacgtgc agctggttat ggtctagggg gtatactcac accgactggt
    11941 attggtactc ttgttgagga gggaaagcaa aaaattgttg tcgatagcaa agaatacctt
    12001 cttgaaaaac caatttatgc agatgttgct cttctatttg catcaaaggt cgataaagca
    12061 ggtaatctag tttataaagg ctctatgaac aattttaata atttaatggc atctgctgcc
    12121 aaaattacaa tagttgaagc agatgaaatc gttgaaattg gtgatattga cccaaatgaa
    12181 gttaatacac caggtatttt tgtgaattac atcgttgaag gaggtaattt ataatggata
    12241 aattagaaat gcaagaatat attgcaaata gagtatctaa agaattaaaa gacggagccg
    12301 ttgtaaacct tggaataggt ctaccaacca aagtagcaaa ttatattccg gataatgtaa
    12361 acgttatctt acaatctgaa aatggctttt taggtttagg ttcagcagaa gatggaaatt
    12421 caagtgatga aactattgta aatgcaggtg gtcaaccagt tactatactt cctggaggat
    12481 gtttctttga tagtgctact tcttttggaa taatcagagg aggtcatgtt gatataactg
    12541 ttcttggcgc acttcaggtt gacaaatatg gcaatatagc aaactatatg attccaggta
    12601 agatggttcc aggtatgggt ggagctatgg acctagtaac tggagctaaa aaagttattg
    12661 tagcaatgga acatacttca aaaggttctg caaaaatatt aaataattgt actcttccac
    12721 ttactgcaac aaatgctgta gatttgatag ttactgaaat gggagttatg gaagtaacat
    12781 cagatggaat tttacttaaa gaaataaatc cagctttcac attagatgat gttatttctg
    12841 ctactgaagc tcctttaata ttgtcagatt cattaaatgg aaaaatatct gttgtttaaa
    12901 ataattaaaa ctagaaaata aaaatctctt agacgtatta catataaatt tgtattgctt
    12961 aaaatattaa tataaaaata aaataaaatt gggggaaatg aaaatggtta aagataaagt
    13021 tgtattcgta acaggagctg caagtggaat aggtaaacaa atagctgaat cgttcttaaa
    13081 aaatggtgct aaagtaatgt tctctgatat aaatcaagaa gctttaaata ataccataac
    13141 agagttacaa aaaaaaggtt atgattgcat gagtgtaaag tgtgatgtaa ctaaggaaga
    13201 agaaattaac cacgctatag acaaaactgt tgaaaagtat ggcagattag atatattaat
    13261 aaacaatgct ggacttcaac atgtttctat gatagaagat ttcccaactg ataaatttga
    13321 atttatgata aaaataatgc tgacagcacc ttttatagct actaaaaaag catttcctat
    13381 aatgaaaaaa caaggctttg gtcgtataat aaatatggcc tcaatcaatg gtgtaatagg
    13441 atttgctgga aaggctgctt ataactcagc aaaacatgga cttattggac taacaaaagt
    13501 tgctgctctt gaagctgccg attctggtat aacagttaat gctttatgcc ctggatatgt
    13561 agatactcca cttgtcagag gccagttcaa tgacctttca aaaactcgta atataccatt
    13621 ggaaaatgtc ttagaggaag ttttataccc acttgtacca caaaaacgcc tattagatgt
    13681 tcaagaaatt gccgactatg ctatgttctt agcaagtgat aatgctaagg gtattacagg
    13741 acaagcttgc ttactagatg gaggatatac tgcacaatag ttattatcac aatagttatt
    13801 atcacaatag ttattatcac aatagttatt atatttaggg cttgaagaaa gtattcttca
    13861 ggtccttcta ctaaggaggg atatttatga gtattatcgc gccaattggt ataattattg
    13921 gtatcatagc tattatatat ttttcagtta aagaaattca tataactata gctgctccaa
    13981 tagcaacttt aatagttgtt attttaaata aaatggatgt tgtaacatca atgctaggtg
    14041 ctgacaaaaa caattatatg ggtgcacttg gaaactatat aatgagttat tttgcaatat
    14101 tcttattagg ctcaatactt gctaaattta tggaagaaag tggagctact gtatcaatag
    14161 ctgattttat tttaaataaa tttggctcta atcatcctta tagagtttta gttgctatat
    14221 ttatagtaag ttttatatta acgtatggtg gtataagctt atttgtagtt atgtttgcgg
    14281 tcattccact tgcaagaact ctatttaaaa aacttgatat ttcttggaac cttattcaga
    14341 taccactttg gcttggtatt gcaacaatta ctatgactat aattcctgga acaccagcca
    14401 ttcaaaatgt tatacctatt caatatctaa atacgtctct tactgcagct actataccaa
    14461 gtattgtagg tagtataggt tgtgctacgt ttggacttgt ctatatgaaa tatgctctaa
    14521 acaaaagtat tagaaaaggt gaaacttatg caacttatac ttcagaatta gaagaagaat
    14581 ctttagatag agaattacca ggattttttg caagtatttt tcctctatta gcactcgtaa
    14641 ttatagctat aactggaagt acttttggtt cagaatttat gaaaaaaaat gtaatttaca
    14701 tagctttaat agttgcaatt cttttatctg cttttttatt ccataaattt ctatcatcta
    14761 agatacagac attgagcata ggtgctagtg gttctatagg tcctatattt gctacatctt
    14821 ctgcagttgc atttggagca gtaataatgg ctgcacctgg ttttgatgta tttagcaaac
    14881 ttataatgtc aataccaggt agtccactta taagtcttac tgttttgact tcaacaatgt
    14941 cagcaataac agggtcctct tctggtgcat taggaatagt aatgccaaac tttgcagatt
    15001 attatttaag tactggtcta aatccagaat taattcaccg tgttgctaca atagcttcaa
    15061 atatactaac tatagttcca caaagtggtg tttttcttac attcttaagc ttatctaagt
    15121 taacacataa aactggattt aaagaaactt tcattgtagt tgcagttggt tcactaatag
    15181 cagaaattat tgtaattaca ttaggattac taatgtatta atataaaaca aattaaaatt
    15241 cataatatga aaggtggtgt ctcaaagtga actttttact taatgagaca ctctttttat
    15301 atgaaataac atatgcctat catttaaaca ataaaaaagg ccttatctct attttgagac
    15361 agaccctttt tatttcttaa agatttaaat aattataatt tgttgctaat acgaaagcat
    15421 ctactgaata aatttaacca aataaaaaaa aatttatcat aactataaat ttacgttatg
    15481 gaattattat aaaaatatta ttttctctaa ctaagtaatt ccatactata caggctttta
    15541 atacaaattg tcaaacacac tgaaatatac ctatttattc catatatatc ttttttacat
    15601 atctttacag taaaaaaagt cttaatctta tgttagtata gattcacggg taacccccta
    15661 cactaaaggt tacaaatcag taacattttt agaattttta gattaagagg tgtttaatga
    15721 tgagaagaaa tataccaaca aaattaaaaa ctatattagc tttaggagtg ttaactttag
    15781 caacagcttc aagtgctgtt actgcaagtg cttcatcact tgaaaataat gtaaatataa
    15841 gtaacaaaag tttagtatat agtaatacta aatacaagtt ttctgcaaaa ccaaattgtt
    15901 ctgtaaaacc agaacttaag cctgataaag ataactcttg taaggataag aatcataatg
    15961 acaagaattg caacaacact aataaacctg aggacaacaa taattcagac tctactaata
    16021 agcctggtaa caataaacct gagtctaata aacctgaaga caacaataat tcaggctcta
    16081 ctaataaacc tgataataat aaacctgaag acaacaataa ttcaggctct actgataaac
    16141 ctgataacaa taaacctgag tctaataaac ctgaagacaa caataattca gactctacta
    16201 acaaacctga tgataataac aactcaggct ctacaagtga aagtttttca gcttaccaaa
    16261 aagaagttgt tgacttagta aatatagaaa gagctaaagc tggattaaat cctcttactc
    16321 ttgattcttc tatatcaaat gttgctacaa aaaaatctca agatatgatt gataacaact
    16381 atttttcaca caattctcca acttatggtt ctccatttga catgttgaaa aaatttggaa
    16441 taagctataa gacagcaggt gaaaatatag ctatgggtca aaaaactcct aaagaagttg
    16501 taaatgcatg gatgaattct gaaggtcaca gaaaaaatat aatgaatcca aatttcagta
    16561 aaataggtgt tggagtagca caaaaaagtg gtggttcaat ttattggaca caaatctttg
    16621 taggataaaa tactcttttt ccatacatat caaaagagta tctctaagtg ataaaaaaca
    16681 caaatgagat actcttttgt atctatttta aaattatcaa atccaaatat acatactata
    16741 aatattattt gttctcttaa ttttagtgtg tagagcaata tataaaaagt attggcaaga
    16801 tgaaaatgga aaacttaaaa agatatttaa taggtaaaaa caggatctta atatcttcat
    16861 aaaaaaaggc tgtctcaaaa tagagataag gcctttttca ttgttgaaat gattaaaata
    16921 tatttgattt catacaaaaa gagtgcctca tgaactaaaa agctcacttt gagacactct
    16981 cttttatcaa ttctatataa gcattataat actttaatat tattcagcta attttttgta
    17041 tccttcatat ctttctttag catcagcttc agctttagca aataatccat cagcttcctc
    17101 tgggaattgt ttagctatag cagaatatct tacttgtgca agtatgaaat ctttgaagct
    17161 tgcagttggt tcttttgaat ctaaagtgaa tgggttttta ccttcagctt ttacttctgg
    17221 attgaatctg tataaatgcc agtatccgca agcaacagct tgtgcttcgt tagctacagt
    17281 tcttcccata ccttctttta atccatggtt tatacatgga gcataagcta ttattaaaga
    17341 tggcccatca tgtttttcag cttctataac ggctttgata aattggtttt tatcagcacc
    17401 catagcaact tgagctacat atacgtttcc atatgtcata gccatcattc ctaaatcttt
    17461 ctttctagat tttttacctg aagcagcaaa cttagccata gcagcagctg gagtagcttt
    17521 agaagcttga cctcctgtat ttgaatatac ttctgtatca aatacaagta cgtttacatc
    17581 ttcaccagat gcaagaacat ggtctaatcc accgtaaccg atatcatatg cccaaccgtc
    17641 tccacctagt atccattgag atctcttaac tagataatct tttctatctt ttatagcttt
    17701 tactaactct ttagcttttt catctgtaca atcacaagct ggtttttcta ataattcaac
    17761 aaccttagca cttgctactt tagaagcttc tccatcattt ctagaatcta accactctgt
    17821 aaatactgct ttagcatctt cacagatatc catagaaact aactctgtca tagcatctac
    17881 tattttattt cttatttgct taacagctgt gtacatacca aatccatatt cagcattatc
    17941 ctcgaataaa gagtttgccc aagatggacc tttaccttca tggttacaag tataaggaat
    18001 tgatggtgca gaagctcccc atatagaaga acatccagta gcattagcta tcatcattct
    18061 atctccatat aattgtgtaa ctactttaat gtatgcagtt tctccacatc cagcacatgc
    18121 tcctgaaaac tccattaatg gttgtctaaa ttgacttcct ttaacagtgt ttgcaggcat
    18181 aacatcacct tttggagcta cctcatcagg atttactgca aatgcccaat tatctaactc
    18241 tgtatcaagt tgagtatcta ttggcttcat aactaaagct ttttctttag ctggacatat
    18301 gtcagcacag tttccacatc ctgtacagtc aagtggacta agttgcattc tatattttaa
    18361 tccttctaat cctttaccta atgctttttt agcttcaaat ccttctggag cattagctaa
    18421 ttcttcttca gtaactaata ctggtcttat acatgaatga ggacatataa aagaacattg
    18481 gttacattga atacagttat ctattatcca ttcaggaaca tcaacagcta taccacgttt
    18541 ttcataagct gcagtaccac aagggaatgt tccatcttct ataccattga atgtacttac
    18601 tggtaagttg tttccttctt gtgcagtcat tggtcttagt atatttttta taaattctgg
    18661 ttctttaact tctcctttat ctgcatcaac agcattttcc caagaagctg gaacttcaac
    18721 tttaactaaa gcatttattc ctgcatcaac agcagcatag ttcatgttaa ctattttttc
    18781 acctttttta ccataagctt taactataga atcttttaag tatttaacag catcttcttc
    18841 tggtattatt tcagctaatt taaagaatgc agattgcatt atcatgttta ttctgtttcc
    18901 aagacctatt tcttgtccta gtttaacagc gttaactgta tagaatttta tatcattttt
    18961 agctatatat tgtttcatat gagctggtaa atgttcttca agtcctgctt ggtcccatat
    19021 agtgttaagt acaaatgtac cacctttttt aagacctttt aataaatcat attgataaac
    19081 atatgattgt ttatgacagg caatataatc agcttcatct attaaataag tagctcttat
    19141 tggagtatca ccaaatctta agtgagacat agttatacca ccagattttt tagagtcata
    19201 atcaaagtaa gcttgagcat atttttcagt atggtcaccg attattttga tagcttgttt
    19261 attagctcca acagtaccgt cagaacctaa tccccagaac ttacatctta tagttccttt
    19321 agaagctatt tttattggct cagatggagt taaagaagta ttagttacat catcaactat
    19381 acctactgta aatccatttt taggttgttc tgaaactaag ttatcaaaaa cagtttttat
    19441 atctgaagga gtagtatctt ttgaacctaa tccgtatctt ccacctatta tcataggagc
    19501 attttctttt ccatagaata catcacgtac atctaagtat aatggttcac cagttgaacc
    19561 tggctcttta gttctatcaa gtacacatat tctttcaaca gtagatggca tagcatctaa
    19621 gaaatgtttc attgagaatg gtctatataa atgaacttta actaaaccat atttttctcc
    19681 cttagcattt aagtaatcta tagtttcttc tattgtttct gttactgaac ccatagctat
    19741 taatacgtat ttagcatctt ctgctccata gtaatcaaat aaactatgtt ttctacctgt
    19801 taaattactc attttttcca tgtatttttc aactatacct actatatttt gatagtattt
    19861 attagaagct tctctagttt ggaaatatat atctgggttt tgagcagtac cacgagcaac
    19921 tggatgattt ggagataaag cattatctct aaatgcttga actgcttcaa agtttaataa
    19981 acttgcataa tcttcatttt ctagtaattc aactttttgg atttcatgtg aagttctgaa
    20041 tccatcaaag aaatgtataa atggtaatct accttctatt gcagctaaat gtgcaacagg
    20101 tgctatatca gctacttctt gaactgaacc agaagctaat aatacacatc cagtttgtct
    20161 agcagccata acgtcttggt gatccccaaa tattgataac gcttgagatg ctaaagcacg
    20221 agcacttaca tggaatactc ctggtaacaa ctctcctgca actttataca tgttaggtat
    20281 cattaataat aaaccttgag atgctgtaaa agtagaagtt aaagctcctc cttgtaaaga
    20341 accgtggaat gttcctgatg cacctgcttc tgactgcatt tcaacaacat taactgtttg
    20401 tccaaatata ttttttcttc cttgtgatgc ccactcatca actacttcag ccatagttga
    20461 agatggtgtg attggatata tagctgctac atctgtaaac gcataggcaa catgagctgc
    20521 agctgtattt ccatcaagtg ttttcataaa cttagccata atatgtatcc tccttgatag
    20581 ctacaattgt acttgtttat aacaagtcta tttttttata tttacatata atttacacaa
    20641 caaaaatata aacacacata tagtcgcgca aatactctat cttctaatat aatactacag
    20701 attagataaa attcctttga aaattgtaaa ttaattacat atttaagtag atttttttta
    20761 aaattaaata tgttaagt
//
LOCUS       DAELJO010000055        20588 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELJO010000055 DAELJO010000000
VERSION     DAELJO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.055"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            137..1168
                     /locus_tag="KPB88_001704"
     CDS             137..1168
                     /locus_tag="KPB88_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0053986.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1301..2599
                     /locus_tag="KPB88_001705"
     CDS             1301..2599
                     /locus_tag="KPB88_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG0053987.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKEAYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2713..2958)
                     /locus_tag="KPB88_001706"
     CDS             complement(2713..2958)
                     /locus_tag="KPB88_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0053988.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3106..3972
                     /locus_tag="KPB88_001707"
     CDS             3106..3972
                     /locus_tag="KPB88_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG0053989.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3976..4512)
                     /locus_tag="KPB88_001708"
     CDS             complement(3976..4512)
                     /locus_tag="KPB88_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG0053990.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4703..4906)
                     /locus_tag="KPB88_001709"
     CDS             complement(4703..4906)
                     /locus_tag="KPB88_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0053991.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5197..5961
                     /locus_tag="KPB88_001710"
     CDS             5197..5961
                     /locus_tag="KPB88_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0053992.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6350..7663
                     /gene="prdC"
                     /locus_tag="KPB88_001711"
     CDS             6350..7663
                     /gene="prdC"
                     /locus_tag="KPB88_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG0053993.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8067..8152
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8734..10983
                     /locus_tag="KPB88_001712"
     CDS             8734..10983
                     /locus_tag="KPB88_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG0053994.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGKVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDVVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            11390..13150
                     /gene="prdR"
                     /locus_tag="KPB88_001713"
     CDS             11390..13150
                     /gene="prdR"
                     /locus_tag="KPB88_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG0053995.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13579..15459
                     /gene="prdA"
                     /locus_tag="KPB88_001714"
     CDS             13579..15459
                     /gene="prdA"
                     /locus_tag="KPB88_001714"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG0053996.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15540..15824
                     /locus_tag="KPB88_001715"
     CDS             15540..15824
                     /locus_tag="KPB88_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0053997.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            15862..16587
                     /gene="prdB"
                     /locus_tag="KPB88_001716"
     CDS             15862..16587
                     /gene="prdB"
                     /locus_tag="KPB88_001716"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16312..16314,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG0053998.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16667..17425
                     /gene="prdD"
                     /locus_tag="KPB88_001717"
     CDS             16667..17425
                     /gene="prdD"
                     /locus_tag="KPB88_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG0053999.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17440..17907
                     /locus_tag="KPB88_001718"
     CDS             17440..17907
                     /locus_tag="KPB88_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0054000.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            17925..18407
                     /locus_tag="KPB88_001719"
     CDS             17925..18407
                     /locus_tag="KPB88_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0054001.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18427..19434
                     /locus_tag="KPB88_001720"
     CDS             18427..19434
                     /locus_tag="KPB88_001720"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG0054002.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19554..20435
                     /locus_tag="KPB88_001721"
     CDS             19554..20435
                     /locus_tag="KPB88_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0054003.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8204 a         2400 c         3834 g         6150 t
ORIGIN      
        1 aataaatata aattaaatat atggtgtact tattttatgt ttttagcaag aaatatagca
       61 tttaaaagat aaattgtgca acttaaatca aagtttaaga tgaactatta acatagatat
      121 attaaaaact tttgtaatgg agaatatatg tgatatgaat aataaagtta aggttgcagt
      181 attagataca ggaatagaca aagaacatga ttacttaaaa gataatttag ttggaggaat
      241 tgcttttgaa tgtatgcatg attatatctt tatatcagat aaatttgatg atgaagatgg
      301 tcatggaact gcttgtgcat caataataaa gaaagagtat gaagatgtag agttatttgt
      361 aataaaaatt ttgagaaaag atggtattac aaatataaaa gttcttgaag aagcattaag
      421 atacctttta gacactaaca taagattaat taatttaagt ttatcagtca taggagtaga
      481 aagtgttaag ggcttatttg aagtttgtta tgaattgttt agaaaaggaa aaataatagt
      541 atgttcactc gctaatgatt ttgacttaag ttatccagct atgtttaaca atgttatagg
      601 tgtaagagga tttactttag atggagatga ttcattttgg tacaacaaaa aatatgatgt
      661 tcaatgtgtt atggatagca attcatatat aagttgtgat ataaataatt cctatagatt
      721 accaccaaaa tgtaatagtt atttatcagc aaagcttact ggtaaaatag ctaagatatt
      781 atcagaaaaa ccaggcataa caatatctga tttaaatgat aagcttgaat cattagcaac
      841 tagaaatcat tgggatagtt gtgattttga tgagtgtagt agaattgtaa actttaaatt
      901 ggatttgtat gacaaagaaa atgctctttt tatgaaagtt gctgacgttg ttagggattg
      961 cttagatatt gaagagaata atgaaaaact ctttaagggt agccttttta ataaggagat
     1021 aggcttggtt tatgataatt gttttaatct gttgaaaaag ctagagcata gatttgacat
     1081 aaagtttaat tatatggaca tctcaaaata tgacttagtt tcaatatata cactgactga
     1141 attagtggaa agatataaaa acttataaat aaagatttgt agctaaattt aatgtagaag
     1201 gtattaaatt atatgtattc tttgtcggta gattaaaagt aaaaaatata ctataattag
     1261 atagcataag agaaaatgcc aatacagggg gagttagata atgaaatatg aagaagcatt
     1321 agagtacata tcacaaacta ataaattcgg tataagatta ggattagaaa atatcggaaa
     1381 gttattagaa ttattaggaa atccacaaga aactttgaat ataatacatg tagctggaac
     1441 taatggaaaa ggttctgtgt gttcttttgt ttctaatata ctaagagagt gtggatataa
     1501 agttggttta tatacatctc catatttaga aacttttact gagcgtataa gagtaaatgg
     1561 acaaaatata ccacaggatg atgttgctag aataattgag ttaataaaag aaaaaataga
     1621 gattatggta aaagaaggct atgcatatcc aacagaattt gaagtggtta ctgcaatggc
     1681 attttattac tattcagaac aaaaggtaga ttttgtggca ttagaagttg gtttaggtgg
     1741 cagatatgat gctacaaata taataacaaa atcacttgta agtgtgatag tatctataag
     1801 tttggaccat acaggtatac ttggagatac aatagaaaaa atagcgtacg aaaaggctgg
     1861 aataataaag gaaaatggag tagtactagt atatgaccag actgatgaag caaaggatgt
     1921 tataaagtca gtttgtaaag agaaaaaagc taaatatata gaagttgatt ttgatgatat
     1981 aaacataaaa aaatcagata taaattctca aatatacgat tgtactgtaa tgaaagaagc
     2041 ttatagagac cttgaaataa aattgatagg cgaacatcaa ataaacaatt ctatcttggc
     2101 aattagtgta ataaaatatc taaaagacat aaataaatta gcaaatataa atgaagaatc
     2161 tataagaaaa ggacttataa atactaaatg gccaggaaga atagaaaaga taaaggaaaa
     2221 tccaattttt ataatagatg gtgcacataa tgaagatgga gcaaaatctt tggcaaaagc
     2281 acttgataag aattttaaag gtagaaaatt aaccttatta ataggtatgt tagaagataa
     2341 agatattgat agtgttttag agatattgtt gccacatttt aataaggtaa taacaacaac
     2401 accaaataac ccaagggcca taaattcaga tatattaaga gaaaaagtat taaaatatgt
     2461 agatgatgta acatcaaagc atgagataga ggatgcagta aattatacat tagaaacatc
     2521 aagtgaggat gatattataa taagtgcagg ttcattatat atgattggta cagtgagaac
     2581 tttagtaaag aaattataat tttataaaat aaatatacaa ataaaaatat aaaaaagagt
     2641 tgtctaaaaa atggacaact ctttttatat aaaataagtt tttaataatt ttactgatgt
     2701 ttatataagt aattatatag attgacgtat agctttagat aattggtctg ggcaagaagt
     2761 accttttcca ccacagtcaa taccatttaa tttatcagca acttcaagag cattttggcc
     2821 aattattaag cttctaagtc ctgataaatt accattacat cctcctataa attcagcatc
     2881 aacaatcaca ttattttcat caacttcaaa aatcatttct ctacaacata caccacttgg
     2941 attaaatgtt actttcatta aaaagacctc cattaaatat tatttctttt tttaacatta
     3001 ataataatac caaatatatg cgtaataatg caactataaa gcataataca agctaatgaa
     3061 ataatatatt attgtagtat atgtattgta gagaggaggg gttatatgaa aatctatatt
     3121 tctaactact tatcaaagag tattagcata gttgattatt ctacacttga actagaaaaa
     3181 gaaatagtat tagaagataa tatatatcca caccattttt gcatagaaaa agaaaaaaat
     3241 ttaatataca tacctagttc aagtaatggg atactctatg tcttagattt aagtaatgat
     3301 aaaataattg atactgtctc tataggagga agtcttagtc aagttgtact tagcgataat
     3361 gaattgtttg tggcaaatga agattctaat agtatatatg tactagataa aaacacatta
     3421 aatccaatag gaattattgg agttgataat atgccacatg gatttgactt tgatagagaa
     3481 agcaaaaagt tatatgtacc gtgtataaac tcgatagtat gtatagatac aattaataaa
     3541 aatatacaaa agaaaataaa tatggacttt agagcttggc atatagaact tgataaacaa
     3601 aagaaagaaa tgtatatatc aacacttgat ggaaagctta taataataga tgaagtaagc
     3661 atggatataa aaaaggtatt atatgaattt ttattaccag tagaaataag atttaattat
     3721 agtggcaaaa aagtttatgt tgctgatttg ggttataata atgtaagaat tttagattat
     3781 acaacgagta gatacattgg aaatattgaa ataaatggga ttccccaagg attagaaata
     3841 tctaaagatg aaaagttact atttgtatca gatacacaag aaaattcagt aaaagtttat
     3901 gaaactgcaa ataacaaatt aataaaagaa atcaaggtag gaaaagagcc tacaactata
     3961 atttgtttgt aggctttaat gtatgtctct tataagtaaa tcgataactt cggcatacat
     4021 ataagatgca ttatttttcc agttattgca aataagtttt gcttgtttct ctgatgaaac
     4081 atttagattt aaatctatta agttagactg attttctatg actctaaggt tgacaataaa
     4141 ttcattatca ctttgtttta caaaactaga ctttacttga gtatctgcta gtagattttc
     4201 tttgttagaa tctatatact tgtctatttt ttcagtaaca ctagtaggta ttcgattaaa
     4261 gaagtattct agactttcta cgcctctttg agtaagagca taatattctc tgtcagagtc
     4321 tttatatgta gtcaaaaact tagattccat aagctgagat aaaagctgtt gtaatgaaaa
     4381 ataattcatc atctcagtct ctaatactac ttgagttatt tgagaattag ttaaatccat
     4441 tttaattttt tctaatatat ataaaattaa aagtttgtga taggctaatt cttcagacga
     4501 gttttcaaac ataaatatca tcctccgtca aactatttct atatataata tattaaacta
     4561 atttgtacaa aattaaaaga acaatttgta ttgtattatt aatctttaat aattcatata
     4621 aaaacaattt tataaataaa tatagaaaaa cagggctgct aattagcagc cctgttgata
     4681 cccaggtgtc aaaatttact atttattttc cagccatttg tctttcagcc atttcaacta
     4741 atttcttagt catatatcca ccaacatatc cattttctct agctgttaag ttacctttgt
     4801 ctatgttttc atagttagat aaacctattt cgttagctat ttctaatttc atttggttta
     4861 aagcagcttt tgcttctgga actactgttc tgtttgaatt tgacatagta aatttcctcc
     4921 tcaaataatt agtttatgat gtctaacaac acttttattt atgtgatgtt aatactaatt
     4981 tctccaaaat aaaaataata tatacagaaa aataaaggta aaaatgtaaa aaatataaaa
     5041 aaattaaata aatgagtatt ttagagagtt taattaggtc aatataggtt ttattttttt
     5101 agttattgtc ttataaatta aatattacag tacttttttg agctgaaaaa aaataaattt
     5161 taaatataga agaaattatt tcaaaggggg aatagtatga aaagagaagt taaaaggact
     5221 aaagctttga tatatagtgt tttatttttt tcattattat tagtgttaat atttgttttt
     5281 gtttatggaa taaaatacat atgttctaaa tctgacaatt caaaaatgcc tatatataaa
     5341 gttgatacaa atgagaaaaa aatagctttg agttttgatg tagcatgggg aactgataat
     5401 atggatgata ttttaaaaat tttagataaa cataatgtaa aagcgacctt ctttttagtt
     5461 ggtagttggg tggatgataa tgaagaaatt gtaaaagcta tagatgaaaa aggacatgaa
     5521 attggaaatc attcaaatac tcatgccaat ttaaaggaaa tatcaaaaga agacataaca
     5581 aaagagatag aaactacatc agaaaaaata tataatataa caggaaaaag gactaattta
     5641 tttagacctc cttttgggga cgtaaacaat aaagctatgg acatttgtag tgatttaggt
     5701 tataaagtaa ttaagtggga tgtagattct atagactgga aggagcttgg accaaatcat
     5761 gtaattgaaa aagtaataaa agaatctcaa cctggttcaa tagttttatt ccatgctaat
     5821 ataaatgatg tagataatta tttggatact ataataagtc gactaaaaaa agatggatac
     5881 agtctagtca aaatatctga tttgttgtat aaagataatt atgtagttga ttccaatgga
     5941 gtgcaaaaaa ctaagaatta ataagtaata aatgtatatt ttaagtaaag ttgtgataaa
     6001 ttatatagta tatgttagaa aagtgtcgat aaaaagacgg tgtctatttt ttgacacttt
     6061 ttatacatgt atgcaatatc aataattata gattaaattg attaaaaaag tgtctaataa
     6121 ttgacttttt tttgatgggt ttaaaaattt agaagaatta atgaatgctg taagacagta
     6181 tttccaaagg gtataagttt gtttaaaagt tggcatagaa attgctttgt taataagcga
     6241 tatgttctat agaaataaac aatatgttaa gaccttataa tagaaaaata gcattaacat
     6301 tatttgaaca gcttataaaa aaattaatat acttaagggg gcgtactagt tgaacaaact
     6361 atattctatt ttattaaaac aacacgttgg tggtccagat aagccagtag taagtgttgg
     6421 agatgtagta aaaaaaggaa ctctaatcgc agaaccaact ggattagggg caaatattta
     6481 tgccagtgtt agtggaaaaa taagtgaaat aaatgaccaa gcgatagtaa ttgaagctga
     6541 tgaagtacaa gaagatactt ttgaaccatt aaaaggagaa ggtatacttg acttaattaa
     6601 agaagctgga gtagtaggaa tgggtggagc aggattccct actcatgtaa aattaaacat
     6661 agatttaaat ggtggaacaa tattagcaaa tgcagctgaa tgtgaacctt tattagctca
     6721 taatataaaa gaaatagaag aaagaccaga aatagtttat caaggtataa aatatgctat
     6781 ggaagttact aatgctggaa aaggtatgct agcaataaaa agtaagcatc caaaagcaat
     6841 agaagctttc aaaaaagtaa taaagccagg agacaacata gaagttgcag aactagttga
     6901 tatgtaccca atgggagaag agagagcaat agtaagagac gttctaggaa aattacttga
     6961 gccaactcaa ttaccttcag aggctaatgc agtagtaata aacgtagaaa cactaactag
     7021 aatagtagag gctgttgagc aaaaaagacc agttatatct aaaaatataa ctgtagttgg
     7081 tcaattaaac agtggaaaag aatctatagt gtttgaagat gtgccaattg gaacaactgt
     7141 cggagaatta attgaaagag ctggtggaat aaaaggtgaa tatggtgaaa taatcctagg
     7201 aggacctttc actggtaaag ctactacttt agatgctcca ataactaaaa ctagtggtgg
     7261 tataatagtt actatgccat ttgttaatga aaagagaaaa atgggtcttt tagtttgtgc
     7321 atgtggacct aatgaagaaa gaatgagaga tatagcaact aaaatgggag ttactgatat
     7381 agtatcagtt caaaaatgta aacaagctca agaaattaaa ggtgcattaa aatgtgaaaa
     7441 tccaggacac tgtccaggac aagctcaaaa atgtattgaa tttaagaaag ctggagcaga
     7501 agttatatta ataggaaact gtactgactg tagtaacaca gtaatgggtt ctgcaccaaa
     7561 attaaaatta ggtgtatatc atattactga ccatgtaatg agaacagtta atcacccatt
     7621 aataagaaga ataaaaggtg acgtaagatt aaaaataaaa taatagataa atagaagtta
     7681 gacatcttta gaagtaaaac ttaaaagtta cttctaagga tgttttttaa tgtattttga
     7741 gggcaatata tatattgtaa aataaatgga aaaaatagac aaaatatgct agatattgta
     7801 taatgataac aatagctatt acaattaata ttggacaaaa ataatgatac tcaagtatgt
     7861 ggtttgtatt aatatgcata ttatattaag tcaactatcc gatagagtaa aaggtttttg
     7921 tgatatttga agatactcta tagtatcttt tttattataa aaactaattt ttgtctttta
     7981 ttagataagt atctaggata ctaatttata ttttattgag ttgttaaaaa agtgcactta
     8041 aaaagttaaa aaagaaaaat taaaaaccta tttatagaaa ctgtgaagta tatcttaaac
     8101 ctgggcactt aaaagatata tggagttagt agtgcaacct gctataaata taaataggag
     8161 taacttttaa ttgtcaaaaa tatataaaat aaatacataa ataagtaatt aatagaagat
     8221 agatataaat atatttttga aatgctccct gcattcataa cgagtgtaga gtggcagata
     8281 tctgcccaaa actcctttaa ttgctggaag ttcctaaagc taactaaacc acaacataat
     8341 catgaaataa ggataagtgt gatagagtgt gcatttaata agcacttgag gttacgaaag
     8401 tagaaaaaat tagttagata gcataaggtt aaatcctaag tgccttgaaa atggataatc
     8461 agcaggtaag cttcgaaaag aagaaacttc aacgactaga gagaaatctc gtacctacaa
     8521 gtgtggggaa atggggagta cctaatttat ggttaggtaa tgatatagtc tgtgcttaat
     8581 agaaatatta agaagttcat aggagaactg gttaggagta acgaccctaa ttgaacacac
     8641 taaagatgta ttattgaata agattcatat aatatttaat aattttaatt atattaagtg
     8701 aatacaatat atgtttttag tatcaggaga attttgaaaa caaaaattaa aaaatcaagt
     8761 ataatcagtt tagtaattgc ttttagtatg gtatttactg catttacacc aatagtatct
     8821 tatgctgacg aagtgactag caatgataca atacttaatg gagaagaaca atcaaatggt
     8881 cagacatcag atgtagagaa accatcagac ggtcaggtac cagatgggga gaaaccatca
     8941 gacagtcaag taccagatgt agagaaacca tcagacggta aggtaccaga tggggagaaa
     9001 ccatcagaca gtcaagtacc agatggggag aaaccatcag acggtcaggt accagatggg
     9061 gagaaaccat cagatggtca gataccagat ggggagaaac catcagatgg tcagatacca
     9121 gatggagaga aaccatcaga tggtcagata ccagatgggg agaaaccatc agatggtcag
     9181 atgccagatg gagagaagac atcagatagt cagacgccag atgtagagaa accatcagat
     9241 ggtcagatgc cagatgggga gaaaccatta gatgagcaaa ctccagaaga agaaaaacca
     9301 ttggaagaag aaatagttat agaggaaatg agtttaaaac aagatattga taaaattctt
     9361 gatatgactt tatctcaaat aaataaaata gtatataact tttgggaaga tgaagaagat
     9421 gtaaaggcag atgaacaatc agaaattaac caagttttta cttcagaaga ctcatttatt
     9481 agcttatggt atgacaaaaa agcaaaagta aaaaattctt gtttactaaa agaaatagat
     9541 ggttcagata gaccttatca tgatttaaga tttgatgata ttacaaagac tttaactttt
     9601 gactatgtaa taaagggtct tgtaggagca agctcaaaag acgacaaata tgtaatagaa
     9661 ggtgaagatg gagagcaaac tgcattttgt tacaataatc atttaagacc accaagtagt
     9721 aatggaaaga gtccatattt accagcagaa gattttaatg gtcaagaaaa ccaaaatgaa
     9781 gaagcagtta aatctatact atatgcaggt tctgaatttg atggatttgg atataagcaa
     9841 caatttaatt taggtggaga agaaaatgaa ttgatgactt actcagctac tcaaagtgca
     9901 atttggatta ttcttggaca aatggatgaa caagaaagac ttaagcagta tcaaggttct
     9961 ataaaccttt gtgataaact tatagaaaga gcaaaaactg aagaggaaag agctgagtat
    10021 cagaagaaga aagaagtagc gataaatata aaagcttatt tagaagcatt gttaaaagca
    10081 ggtcgtgaag agttaaagcc aaataataca ggaaaaccta gtttgagtaa tggtttaact
    10141 aagattaact ttgaaaaaaa tgaagatggt acttatgaaa cagaagctgt agccttagta
    10201 ggttatagtg gtgtagtaaa gttaaaacta ccagatggag taactgctta tgatggagat
    10261 ggtaatataa taggtacagg cgaagtagaa atttcaacgc aacagaaatt taaacttaag
    10321 agtgttggaa aaccagatgc caaagctaat atttctgctg tatcttatga ttatattttt
    10381 ccaaaagcta ttcaatatta taaggcagtg cttgatttag gacaaaaaga ccattcatct
    10441 tcattaccat ctagcaagca aaatttatta agttatacaa tagagaaaaa gaatggtcaa
    10501 gaagttaact ttaatatagg tttaccaaca gatgatgatg ttgttgtaaa tccaccagtg
    10561 ccaccaatag atgatgatgt tgtaaatcca ccggtgccac caacagatga tacaaatgga
    10621 cataagccaa aaccatcacc accaatagat gatacagtaa taaatccacc agtaccacca
    10681 atggatgata caataataaa tccaccagta ccaccaacgg aggatgcagt gttaaatcca
    10741 ccagtgccac caatggagga tgcagtgcta aatccaccag tgccaccaac agaggataca
    10801 gtgttaaatc caccagtacc atcagtaagt gatacagtgg aaaaaactcc agaactatca
    10861 agagatgata caataataaa atcaccaaaa acaggtgatg aaactcagct aatgtcatat
    10921 gtatttatta gtgtcattgc aatttgtgga ttagcgtatc aatgtaaaat aaaaagaaat
    10981 taatctagat tattattaag tttaaaataa aaagtctcaa attgatttat aagtcatttt
    11041 ggggcttttt tatgtaaata taatatgcaa atatagtttg tttaaaaata tctattttaa
    11101 ttatattctt aaatttatta ttggatacag gcattcatta attgtttgat gaatagaaaa
    11161 atgttttgaa ggtgaaaata gtattgaaaa aataatatac taagggtata attcatttga
    11221 tttcatttgt ataaatttaa ttataaattt taaagtgatt atataaattt aaatgctaat
    11281 tacttaataa attttaataa taattattta ataaataatt attataatat gctagaataa
    11341 atatatataa ataagttttg atgaggtact actataaagg ggggacttta tgttttcgat
    11401 accagaagta aaaaaagtag aagaggtaat ggatacaaaa tttacaacta tagacgaaga
    11461 cacaagaatt gaagatgcaa taaaagaaat gataaaaagc aacacaaaaa ctctaatggt
    11521 aatagattca tctgaccaat taaaaggcat tatatccatg acggatatac ataatcttta
    11581 tgagatgcat aaaaagtatg aaggtcaacc agtaaaactt ataatgaaga aggatgtaat
    11641 atatgtcaac gaaggtttaa ccttagatga atgtagagat ataatgatat taaaaaatat
    11701 agggattctt cctgttctta gagataataa aattattgga gttctaaaac aagagcacat
    11761 tagggactat ctatatatgc atttagaaga ttatggatta actttgaagt atattatagg
    11821 tcaaattaaa gaaggaattt gtgctataaa taacgaaggt gttgttatct tgtggaataa
    11881 gtttatggag gaaaggtatg atataaagtc tgaagatata gttggtcgac cgatgaatga
    11941 atttttggaa aatactattt cagaaaaagt actaaatagt aaagttggaa tgagtgactt
    12001 gtattttaca gataaaaaag aaaatatgta tgcattagtt catgcaaatc ctatatttta
    12061 taaagaagaa tttatcggtg ttgtatgtac agaagttgat gttacagaag ctaagatatt
    12121 agctcttgaa cttgaaaaag taaatgatac attaaaatat ctaaagaacg aagtcaaaaa
    12181 tttatctaaa ggtagttttg ataagatttt aggtaaaagt tataaattgg aaaaatcgaa
    12241 agcgattgca aaacaagtgg ctagaacaaa tagtagtata tttatttggg gcgaaagtgg
    12301 tacaggtaaa gaagtatttg caagagctat tcatgattat agtgagagaa aagggcaatt
    12361 tatacctgtt aactgtagtg ccataccaaa tgagttattt gaaagtgagt tttttgggta
    12421 tgaatcaggt gcatttacag gagcaagtaa aaagggtcga ataggtatat ttgaacttgc
    12481 aaaagatgga actgtatttt tagatgaaat tgccgattta cctcttagta tgcaagcaaa
    12541 gttgcttaga gtattacagg aaaaagaaat aagaagagtt ggtggagata ccacaataaa
    12601 aataaatcct agaataatat cagctaccaa taaagatttg agtaaaatgg taaaggcaga
    12661 aaaatttaga gaagatttat actatagatt aaatgttgtt gaaataaaga ttcctccact
    12721 tagagagaga aaagaagaca taggattatt ggttcatagc tttttagagg agatatgtaa
    12781 acaaaataat aagcctgttc ttacaattag caaagatgta atagatatat ttcagaatta
    12841 tagatggaaa ggtaatatta gagaattaaa aaatacgatt gaaaatattg ttgtattgtc
    12901 acaaaattct aaaatagatg tggatgatgt accaagttat atgatggatt ctacaaataa
    12961 tagtactgaa gaagaagagt atcctttaga tttaacgaag gcaactcaaa aaattgaaat
    13021 aaagaatata accaaggcac ttaaaatgtc taatggaaat aaagctaaag cagctaagat
    13081 attaaatata cccagaacaa ctttgtatta taagatagac caatacaaaa tagatgtgtc
    13141 taaaatatga cacatgacta aattttgaca aaatgataaa ctttaattta aaacctagta
    13201 aagccaacac ttgtataagt gttgtgtata tagatgtgtt aaaaaaaatg aaaaagtgtt
    13261 gattaattga cacttttttt gtttgagaaa atgtttacaa atacaaaaaa taacatattt
    13321 taaataaata tttatataat atttggctaa aaatagcgat agatgaagaa tctattatag
    13381 aaaaaatctt tttgttaata agttggcata ggaattgctt ataatataaa gtgactttaa
    13441 gtgagaaaaa aataatatta atcataacaa attatgtagg ttttaattta cttaatttct
    13501 cagttaaggg gttacctgtt gaggaattga gatttaaaat aataaataag aaaataatgt
    13561 aaggaggtat tttatactat gtcaataact ttagaaacag ctcaagccca tgcaaatgac
    13621 ccagcagttt gttgttgtag atttgaagcg ggaacaatta tagcgccaga aaacttagaa
    13681 gatccagcaa tatttgcaga cttagaggat tctggattat taacaatacc agaaaatgga
    13741 ttaactatag gtcaagtact aggagctaag ttaaaagaaa ctttagatgc actttctcca
    13801 atgactacag ataacgtaga aggatacaaa gcaggagagg ctaaagaaga agtagtagaa
    13861 gaaacagtag aagaagcagc tccagtatca gaagcagcag tagttccagt aagcacagga
    13921 gttgcaggtg aaacagttaa aatacacata ggtgaaggta agaacataag cttagagata
    13981 cctttatcag tagctggtca agcaggagtt gctgctccag tagcaaacgt tgctgctcca
    14041 gtggcaagtg cagcagcaga agtagctcca aaagttgaag aaaagaaact tttaagaagc
    14101 ttaactaaaa aacactttaa aatagataaa gttgaatttg ctgatgagac taaaatagaa
    14161 ggaactactt tatacatcag aaacgcagaa gaaatatgta aagaagctaa tgaaactcaa
    14221 gagttagttg tagatatgaa gttagaaata ataactcctg ataaatatga aacttacagt
    14281 gaagctgtat tagatataca accaatcgct actaaagaag aaggcgaatt aggttcaggt
    14341 ataactagag ttatagatgg agctgtaatg gtattaactg gtacagatga agatggagtt
    14401 caaataggtg aatttggttc ttcagaaggt gagttaaata ctactataat gtggggtaga
    14461 ccaggtgctg ctgacaaagg tgaaatattc atcaaaggtc aagtaacaat aaaagcagga
    14521 actaacatgg aaagaccagg acctttagct gctcaccgtg catttgacta tgtaactcaa
    14581 gaaataagag aagcattaaa gaaagttgac aactctttag tagttgatga agaagtaata
    14641 gagcaataca gaagagaagg taaaaagaaa gttgttgtta taaaagaaat aatgggacaa
    14701 ggtgcaatgc atgataacct aatattacca gttgagccag ttggtacatt aggagctcaa
    14761 ccaaacgttg acttaggaaa catgccagtt gtattatctc cacttgaagt attagatggt
    14821 ggtatccatg cattaacttg tataggacct gcatcaaaag aaatgtcaag acattactgg
    14881 agagagccat tagtaataag agctatggaa gacgaagaaa tagatttagt aggtgttgta
    14941 tttgttggtt ctccacaagt aaatgctgag aaattctatg tatctaagag attaggtatg
    15001 ttagttgaag ctatggaagt tgatggagct gtagtaacta ctgaaggttt cggaaacaac
    15061 catatagatt tcgcatctca catagagcaa ataggtatga gaggtatacc agtagttggt
    15121 gtaagtttct cagctgttca aggtgctcta gttgttggta ataaatacat gactcacatg
    15181 gtagacaaca ataagtctaa gcaaggtata gagaatgaaa tattatctaa caacacttta
    15241 gctccagaag atgctgttag aataatggct atgcttaaaa atgctataga aggtgtagaa
    15301 gttaaagctc ctgaaagaaa atggaatcca aatgttaaat taaataacat agaagctata
    15361 gaaaaagtta caggagaaaa aatagtatta gaagagaatg agcaatctct accaatgagt
    15421 aagaagagaa gagaaatata cgaaaaagac gaaaactaaa atttagatac taactaagat
    15481 ttagacaata acataaattt agatccaagt ttaaaacagt atagagaggt agaattataa
    15541 tggataggtt aaaatatatc tcatcagaga ctttttatga aggtattata gttgacatca
    15601 aaggcggtgg agtgactata gacctaaaag gtagacttgg acaatttaaa atacctaata
    15661 gaatgcttat aactgactac gaacttaaga taggtcaaga agttggattc atgctaagct
    15721 atcctgaggt attaagtcca gagcctaatc aagagtatgt agaaaatatt aggagagaaa
    15781 aagaaaaaca agcagaaatg caatcaaaac aagaagaaaa ataataaata atcgtttgaa
    15841 aatagaaggg agaggaaata tatgagcctt acaacagtac aaggacttca atctgaaata
    15901 ttcgttccaa taacacctcc tccagtttgg actcctgtaa ctaaagaatt aaaagatatg
    15961 actatagctt tagctacagc agctggtgtt catttaaaat ctgacaagag atttaatcta
    16021 gcaggagata ctacatttag agctatacct aatacagcta cagttgatga aatgatggta
    16081 tcacatggtg gatatgacaa tggagatgta aacaaagata taaactgtat gttccctata
    16141 gatagattac atgaattagc tgcagaagga tttatcaaag gagtagctcc tatgcactat
    16201 gcattcatgg gtggtggagg aaaccaacat gtcttcactg aagaaactgg tcctgctatc
    16261 gctgctaaac ttaaggaaga gggagtagac ggtgtagtta tgacagctgg ctgaggtact
    16321 tgccatagaa ctgccgtgat cgtgcagaga gcaatagagg aagctggaat acctacaata
    16381 ataatagcag ctcttcctcc agtagttaga caaaacggaa ctcctagagc agttgctcca
    16441 ttagttccaa tgggtgctaa tgctggtgaa ccacataata tagaaatgca aacacatata
    16501 ttaagagata cattagagca attagttgca ataccatctg ctggtaagat agttccatta
    16561 ccatacgaat ataaagctca cgtttaaaat gtaatttata tttgaaaata tagaaccctt
    16621 ctcctacacg gttaggagag gggttttaag aacaaaggtg gatattatgg aagaaaaaat
    16681 aatgagaaga cttgttataa aaccgtttca tatgaatgaa gttaatttcg gttcaaaaac
    16741 ttctataaag aaagatgtac ttacaataga tttatcaagt atagatgaaa taaaagaaag
    16801 agaagagtta ataacagaca taaaagtaga cattataaaa cctggagatt atgacagaga
    16861 aattaatact attatggaca ttataccaat atcaacaaaa gttttaggca gactgggaga
    16921 gggtattaca catactttaa cgggtgtata tgtaatgtta actggtgcag atgaagatgg
    16981 aaatcaaatg catgaatttg gttcttcaga aggaattttg aaagaccaaa tggtatttgg
    17041 gagatatgga actccatcag ttgaagatta tataatccat gtagatgtaa ctttaaaggg
    17101 aggtttacct tttgaaagaa cacttccact tgcagcattc agagcatgtg atgattttat
    17161 acaagaaata agagcgtctt tgaaaatgga agacggaaga aatgctactc aagttcgtga
    17221 atattttgac aaaattcgac caaatgcaaa aaaggttgtc atagtaaaac aaatagcagg
    17281 tcaaggtgct atgtatgaca atcagttatt ctcaaaggaa cctagtggat ttgaaggagg
    17341 aacatcaatt atagatatgg gaaatgtacc tatgattata tctcctaatg aatacagaga
    17401 tggtgccctt agagctatga cttaagagag gtggatatta tgggaatagg accttcaaca
    17461 aaagaaacat ctcttcatca ctttagagac ccacttttag acatagttga aagtgataag
    17521 gatgtagatt tacttggagt aatagtagta ggaactccag atggaaatga aaataaaacc
    17581 tttgtgggac aaagaacggc cgcttggctg gaagccatga gagtagatgg agctatagtt
    17641 tcatcagatg gttggggaaa ctcacatgtt gactatgcta acacatttga ggaaatagga
    17701 aaaagagata tacctgtagt tggagttaca tttaatggga ctcaagctaa gtttgttgta
    17761 agcaatcaat atatggatac catagttgat atgaacaaat caaaagaagg tattgagaca
    17821 gaagtagttg gagaaaacaa cacaaatgaa atagatgcta aaaaagcttt ggcattttta
    17881 aaacttaaaa tgagaaaaca tggataaaaa atttaagtaa gggaatgagt tttgtgggaa
    17941 tttctacatc aatgaaaaac acatcacttc atatatttag agacccgtta ctagatttga
    18001 tagataatga ttctgacata gacttgttag gagtggttgt agttgggaca tcagagcaca
    18061 atgaatggaa aacattttta gcaactagac ttggaagatg gattgaatca ttgcgacctg
    18121 atggagtgat tataacttta gatatggcag gaaaccagca tattgatttt acaaatgcta
    18181 tagcagagtt tgtaaaatca gatataccta cagtaggtct tactattatg ggagcagatg
    18241 gattagtaat taccaatcca tatttggata aagctaccat aattgattat aaaaagacaa
    18301 caggctacat agaaacagaa gtagttggag acaatcacat ggatgaagtt gatgttaaaa
    18361 aagcattggc tttcctaaaa ttaaaaatgc gtaaagacgc aaaataaacc taagggggaa
    18421 tttgatatga aatttagcag aagtatacaa gctatagact ctcatacagc aggtgaagca
    18481 actagaatag ttgtaggtgg aatacctaac ataaaaggaa attctatgcc tgagaaaaaa
    18541 gaatatttag aggaaaatct agattattta agaactgcta taatgttaga gccaagagga
    18601 cataatgata tgtttggttc tgtaatgact caaccttgtt gcccagatgc tgactttgga
    18661 ataatcttca tggatggtgg cggatacctt aacatgtgtg gccatggtac aataggagct
    18721 atgacagcag ctatagaaac aggtgtagtt ccagcagtag agcctgtaac tcatgtagtt
    18781 atggaagctc cagcaggaat aataagagga gacgttacag ttgtagatgg aaaagctaaa
    18841 gaagtatcat tcttaaacgt accagctttc ttatataaag aaggtgttga agttgactta
    18901 ccaggtgttg gaactgttaa atttgacata tcatttggtg gaagcttctt tgctataata
    18961 catgcaagtc aattaggttt aaaaatcgaa cctcaaaatg ctggtaaatt aactgaacta
    19021 gctatgaaac ttagagatat aataaatgag aaaatagaaa tacaacatcc aactttagct
    19081 catataaaaa ctgtagactt agttgaaata tatgatgagc caactcatcc agaagctact
    19141 tacaaaaatg tagttatatt tggtcaaggt caagttgaca gatctccatg tggaactgga
    19201 acaagtgcta aattagctac tttacatgct aaaggtgaat taaaagtagg agaaaaattc
    19261 gtatatgaaa gtatattagg aactttattc aaaggtgaaa tagttgaaga aactaaagtt
    19321 gcagatttca atgctgtagt acctaaaata actggttctg cttatataac tggatttaat
    19381 cattttgtaa tagatgaaga agacccactt aaacatggat ttattcttaa ataagtaata
    19441 aataaatcag gaaattaaaa tagaatagta gccacttgtc ggttgctatt ctatagcttt
    19501 taggctataa gattcagaat atatgaaaaa ataaaaaaat aggaggatgg attatggtag
    19561 caatcttaaa ggcattatat gtagtattcg cagcaatctt tgcagttgtg tttggtaaag
    19621 acgtagctaa agcaaacaaa gaggggaaac tagaagaaca aagtactcca aaagttgctg
    19681 taacaggatt tataacagac ttctttgata cattaggaat agggtcattt gctccaacaa
    19741 tagcaatgtc aaaggcttta aagttaaata taccagataa aaaaatgcct ggtacattaa
    19801 acgtagcaca tactattcca gttgttacag aagcattcat atttacatca atcataccag
    19861 ttgatggtgt tactttagta tctatggttg tggcagcagc agttggttca tatataggtg
    19921 caggaataat agctaagatg gacgagagaa aaatacaact tgttatgggt gtaactttag
    19981 cattaacagc aatattaatg ttattaggtc atccttggat taacgtatta ccaggtggag
    20041 gaaatgaatt aggtcttact ggtgttaaat tagtaatagg tgtaataggt aactttatac
    20101 taggagcttt aatgacagct ggtataggtc tatatgctcc aggtatggca atggtttatt
    20161 tcttaggaat gtctgcaaaa gttgcattcc caatagttat gggttcttgt gcattattaa
    20221 tgccagttgc aagtatgaaa ttcataaaag aagatgcata tactaaaaaa gcatcagtta
    20281 taatagctat atgtggttta ataggagttt tcgtagctgc atatatagta aaaactctac
    20341 caatggatat attaaaagca ttagtaatag tagttatagc ttatacttct gctacaatga
    20401 taatggctgc aaataaaaac aaaaagactg cttagttaaa gactacttag tgtttattaa
    20461 atattacagc taataataaa gattataaac tgctttaaaa tattgaatta atattttagg
    20521 gcagtttttt atttactaaa ttaatataaa gcaattaaca tgccaattat gtaatatata
    20581 tagtgatt
//
LOCUS       DAELJO010000056        20091 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELJO010000056 DAELJO010000000
VERSION     DAELJO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.056"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            215..670
                     /locus_tag="KPB88_001722"
     CDS             215..670
                     /locus_tag="KPB88_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0054004.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            719..1303
                     /locus_tag="KPB88_001723"
     CDS             719..1303
                     /locus_tag="KPB88_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG0054005.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1308..1631)
                     /locus_tag="KPB88_001724"
     CDS             complement(1308..1631)
                     /locus_tag="KPB88_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054006.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1783..2301
                     /locus_tag="KPB88_001725"
     CDS             1783..2301
                     /locus_tag="KPB88_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0054007.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2477..3145)
                     /locus_tag="KPB88_001726"
     CDS             complement(2477..3145)
                     /locus_tag="KPB88_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054008.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3260..4675
                     /locus_tag="KPB88_001727"
     CDS             3260..4675
                     /locus_tag="KPB88_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0054009.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAGFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNMEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4898..5356
                     /locus_tag="KPB88_001728"
     CDS             4898..5356
                     /locus_tag="KPB88_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054010.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5353..5937
                     /gene="clpP"
                     /locus_tag="KPB88_001729"
     CDS             5353..5937
                     /gene="clpP"
                     /locus_tag="KPB88_001729"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0054011.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6249..7325
                     /locus_tag="KPB88_001730"
     CDS             6249..7325
                     /locus_tag="KPB88_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0054012.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7392..9356
                     /gene="bclA3"
                     /locus_tag="KPB88_001731"
     CDS             7392..9356
                     /gene="bclA3"
                     /locus_tag="KPB88_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG0054013.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGATGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGNTGADGATGPTGATGATGA
                     DGATGPTGATGATGVTGATGPTGATGATGATGASAIIPFASGIPLSLTTIAGGLVGTP
                     GFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFSTTAALSLVG
                     STITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAQT
                     RFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9525..10286
                     /locus_tag="KPB88_001732"
     CDS             9525..10286
                     /locus_tag="KPB88_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0054014.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10595..10873
                     /locus_tag="KPB88_001733"
     CDS             10595..10873
                     /locus_tag="KPB88_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054015.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11166..11741
                     /locus_tag="KPB88_001734"
     CDS             11166..11741
                     /locus_tag="KPB88_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG0054016.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKRLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12180..12890
                     /locus_tag="KPB88_001735"
     CDS             12180..12890
                     /locus_tag="KPB88_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054017.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12884..14200
                     /locus_tag="KPB88_001736"
     CDS             12884..14200
                     /locus_tag="KPB88_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0054018.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGFAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14212..14352
                     /locus_tag="KPB88_001737"
     CDS             14212..14352
                     /locus_tag="KPB88_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0054019.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14411..14992
                     /locus_tag="KPB88_001738"
     CDS             14411..14992
                     /locus_tag="KPB88_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0054020.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            15139..16848
                     /locus_tag="KPB88_001739"
     CDS             15139..16848
                     /locus_tag="KPB88_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054021.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITVIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNIKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSYMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            16863..17735
                     /locus_tag="KPB88_001740"
     CDS             16863..17735
                     /locus_tag="KPB88_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0054022.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRDVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            17736..18524
                     /locus_tag="KPB88_001741"
     CDS             17736..18524
                     /locus_tag="KPB88_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054023.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(18620..19453)
                     /locus_tag="KPB88_001742"
     CDS             complement(18620..19453)
                     /locus_tag="KPB88_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG0054024.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMHKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(19521..>20091)
                     /locus_tag="KPB88_001743"
     CDS             complement(19521..>20091)
                     /locus_tag="KPB88_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG0054025.1"
                     /translation="LKELDPHTISNLSNIPFNNDLNRFTLNFFDRQYIIDYPSGEVWN
                     SNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIPGGNVYYPNFLNRTLYRLSEV
                     FTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMIFILWFGDDEFPPNSNILFDS
                     NIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         7879 a         2275 c         3334 g         6603 t
ORIGIN      
        1 aatgatatat taaggttcta atatattata caaaataaaa acattaattc aacttaaata
       61 attttaagta ttatatgtgt tttgattaaa gaaaactaaa attttaagga aaaataacat
      121 atttgtatta aattttattg aaatgattaa atttatttac ttaatgtaaa ttattagcta
      181 aaattgtatt aaataattaa ggaggtgagt agctctggat gaaatagatt taaaaataat
      241 taattctcta aaaaagaata gtagggcttc aacctctgaa ataagcagac aaattaattt
      301 atcagtacca gcagtctcag agcgtataag gaaaattgag cagtcaaatt taattcaaaa
      361 gtacactatt caaattaatc gggaaaaaac aaaatataag cttttggctt ttatatttat
      421 caatactgat ttatctaaaa gtatgaaaga atttaagaaa actatattgg aatatgatac
      481 tgtattagaa tgtcatcata tttcaggaga gtatgattac ttaataaaag tattagcaga
      541 agatacaagt aatcttgaag agtttatatc agtgactttg aaggaaattg gggggattca
      601 aaaagctaat acaaccattg ttttatctac aattaaagaa gaatttaata caataaaaga
      661 tattttttaa ataatttact agtaaagtgt taattatatt ttaggaggaa aatagaatat
      721 gaaagtttta gtttttttag caaaaggttt tgaaactatg gaatttagtg tatttgtaga
      781 tgttatggga tgggctcgta acgattatgg gcatgatatt gatgtagtga cttgtggatt
      841 taaaaaacaa gttatgagca catttaatat acaagtatta gttgataaga cgatagaaga
      901 agtatgtgtt gatgattatg atgctctagc tattcctgga ggatttgaag aatttggatt
      961 ttatgatgaa gcatatgatt catctttttt aaatctaata agggagttta attcaaaaga
     1021 aaaaataatc gcttctattt gtgtggcagc attgccagtt ggaaaaagtg gtgttttaaa
     1081 aaatcgtaaa gctactacat atcatttgaa aaatggtaaa agacaaagac agttaagtga
     1141 gtttgatgta aatgtagtta atgaaccaat tgttgtagat aaaaatatta taacctcata
     1201 ttgtccagaa acagctccac atgtagcttt taagctttta gaaatgttga catctaaaga
     1261 acaaatggat gaagttaaat tagcaatggg atttaaactg taatggttta tagattttct
     1321 aagtaatcag aaccccagtc acacatggaa tctaaaattg gaataatact ctttccaaaa
     1381 tcagatagcg agtattctac ttttggagga acaacaggat aaaccactct tataattaaa
     1441 ccatctgatt ctaattcacg taattgttgc gttaacatct taggcgttgc tttaggaatt
     1501 aattttttta attcattgaa cctcaatata gtgtctttaa gatgccaaag tataagtgtc
     1561 ttatatttac caccaatcaa tgcaagtgtt gcttctattg ggcaactgta attatctaaa
     1621 cattttttca tttttgggct cctttattta ttttaaagta tctttttagg tactatatta
     1681 caaaaaagtg cattattgta atatagtacc atagtgataa aattatagca taggttaaat
     1741 aaatcatcaa cttagcctaa taaaacaata gggggtaaat aaatgaattt cgttgaactt
     1801 gcaaaaaaaa gatattcttg ccgtaattat caagatagaa aggttgaaaa agaaaaactt
     1861 gagaaggttt tagatgttgc aagaatagct ccaactggtg gtaatcgtca acctcaaaga
     1921 ttgattgtta ttcaggaaaa agaaggtata aataaacttt ctaaggctgc caatatatat
     1981 gatgcgccat tagcaattct tgtatgtgga gataaagaca aagtttggac aagacctttt
     2041 gatgggaaac aacttacaga tattgacaca agtatagtta cagaccatat gatgttacag
     2101 gcaactgaac ttggattggc aagtgtttgg gtgtgctatt ttaatccaga tataattaga
     2161 gaagagttta gtttgccaga taatctagaa ccaataaata tattattgat gggatatgaa
     2221 tctaagatac cagagagccc agagagacat gaaaaaactc gtgttccttt aagtgaaatt
     2281 gtttcttatg aaactcttta attataaaat taagttattc ataggataaa tgtaataaag
     2341 tgtttattat aaacaaaatt caaaataaat aatatctaca tataattaat tttagtgaag
     2401 tatatctagt ttaactagaa tgtatacttc actaatttat ttattcaaaa tatttatgag
     2461 gattttaata tattaactaa aatatatcaa ttgaattaac tttcaaatct ggaaaagcac
     2521 ttgaaatata gtgttttata tcatttaaat ggtcttgaat ttgcttttct gatattttat
     2581 caggtccata tacaaaatac ataatatttt tagtatcttt tgttatagct gttcgattat
     2641 cagggccatt aagaatacta gataatatac caatattatc tgataaaaat aaatcatctt
     2701 ttgttaaaat atgttcttta ccacctatac cataaaaatg ttcacttcca tttgctaatt
     2761 ctattttcaa aggtaattcc attttatcaa ggtcatgacc tgctgttaga agaagatttt
     2821 tcaattctgc taaaaacatt gactcaattg ttacacctac atctgggata ccttttgatt
     2881 ttaatagaat agattctaat tgaagcaata cagggtaagt tttcttgaat tttttataat
     2941 actttatata acttgaaaca ggctctgttt taattttttc tttacgattg tattctgggt
     3001 gttgttctat aatattttta attgtagagt ttttaagaac taaaaaattt tctttattag
     3061 gttttgaatg tgtaatattt ataaccataa ctccaaattt aatattggga taaatttctt
     3121 taactttatg tgatatttct aacataatat agactccttt ttattttata ttttatgata
     3181 taatcttagc aaattaaaag tggattttgt agttccattt ttatagtaat tttatagaac
     3241 cacttttgga ggtattagaa tgtggattac gattaataga aatagtaata tatcattatc
     3301 aagacaaatt tattgtgaga taaaagatat gattctggat ggaagcttaa agtttgagca
     3361 aaaactgcca tcatctagga ctttatcaaa agaactatct gtatctcgta atacagtttt
     3421 agatgcatat aatcaattaa ttgcagaagg atatttggaa acaaggaatg gatatggaac
     3481 aattgtagct aaaggaatat cagggcataa agttgatttt aagttaaatg atacttttag
     3541 agatgaaaaa gatttacata ctaataaaca aaaaaattat atagattttc gttcaggagt
     3601 tccagaatta aaactatttc cacgcaaaga actttcaaag ctatataaac aaatactgta
     3661 tgatttatca gattcggatt tcagatatgg aagtacagct ggtgtatggg ttttaagagt
     3721 ggaaatatct aagtatttat ttcgtactag aggaattaga tgtaatccca aaaacattat
     3781 gattgtttct ggttcaacac aaggtttatc tttaatttca cagcttttat acaaaagtaa
     3841 taaaaaggtt gctgtagaag accctattca taaaggctta ttaaaggtta tttcatctat
     3901 tggatattca gttgttggaa ttaaagtaga tgataaggga atgaatacca acttaataaa
     3961 atcaataaag gggatttctt ttgtttatac tacaccttct catcaatacc cattaggagg
     4021 gattttacct attcaaagaa gactagagct tattaaatgt gcaacagaga atgattacta
     4081 tataattgaa gatgattatg atagtgaatt tagatatgag ggtcaaccaa ttagttctct
     4141 ttatgaactt agcccaaata aagtaattta cataggttct tttagtaaaa tattatctcc
     4201 agctattcgt gttggattta tgttattacc agacaatatt ttagaaagct ataaaacctt
     4261 aaaaatgtac tctgatgttc atacagaaac aatattgcaa catgtattag ctggatttat
     4321 tggaaatgga ggtttagaga agtacatatt tagaatgaag aagttgtaca atagaaaacg
     4381 taagtacctc ataaaagttt taaacatgga atttaaaaat gaatttaaaa taaaaggtga
     4441 agcagcaggg cttcatatag ttgctcattt ctataatgtt aaatttacgg aaagtttaat
     4501 tcataaaatt tttgaagcag gtataaaagt ttatcctata caacaatatg caattgagaa
     4561 tatattagaa tatgaccatg aaattatact aggttatgct catcttagtt ttgatgaaat
     4621 tgaaaaaggt gtaaaaatac tcagcaaaat aatacatatg aatcaaggta catagataaa
     4681 acatgtagca atctacatat aatagatgta attaagttta aactttaaaa gaaatatata
     4741 ttaatatttt tgtataaaaa tacctgatat ttttttatcg atactttatt gctatacact
     4801 aaattaagat agcaatttat attaagaaaa aactttttgc ataggttata atacttaaaa
     4861 ggagaggata gagttgaaaa caaatacaga tgttattatg gagataattg attatataga
     4921 agaaaatctt acagaaaatc taaatttaga taaaatagct tatattgcta attattcaaa
     4981 ataccattta cacagaatgt ttacaaatgt tgtaggatgt actatccatc aatacataca
     5041 gaggaggaga cttactgaag cagctagaca acttatattt acagacaaat atattataga
     5101 catagctatt acagtaggat attctacaca tcaatctttt acactagcat ttagaaattt
     5161 atataaagaa tctccacaag tatatagaaa taaagatgaa ttttatccta ttcaacagag
     5221 atttgtttta aatctaaatc atcaaattca aagcgaaaaa tgtatttctt atgcaaatat
     5281 gaattataaa caaagaagag atgaaatcaa ttcttatctt aactttaata ttaattgtga
     5341 ggtgaaagta gcatgagttt agtgccacat gtaattgaac aaacaggtca aggggaaagg
     5401 tcttatgata tatattcaaa attattaaaa gatagaatta tatttatagg agaagaaatt
     5461 aatgatgcta ttgcaagttt agtagtagct caattactgt ttttagaatc agaagaccca
     5521 gattcagata taattatata tataaatagc cctggtggct ctgctacatc aggatttgct
     5581 atatatgata ctatgaatta tgtcagatgt gatgtatcga ctatttgtat tggtatggct
     5641 gcaagcatga gtgctttttt acttgcagga ggaacacatg gaaaaagatg tgctttacct
     5701 aattcagaga ttatgattca tcagccaatg ggaggagcta aaggtcaagc aacagatgta
     5761 aaaatagctg tagataatat attgaaaata aaagaacgat tagataaaat tctttctgaa
     5821 aatacaggta aatcaataga agatattagg agagatacag atagagataa ttttatgact
     5881 gcactagagg ctaaagagta cgggctgatt gattatataa tgaataggaa tgaataaagt
     5941 atcaaagtat catattaaaa tgtctgtttt acaagtatat atataatatt aaataagagt
     6001 gtctcaaaat gaacttttta atttatggga cactcttttt tgtatgaaat taaatatggt
     6061 tttatcattt caacaattat taccaaattt caagtttaat aaattctact ataaaaaata
     6121 cataaaagat aaggttcttt agtaactttc tgtcgtcaaa agtttttata aatttaataa
     6181 caaaacaaga gaaaatacca tatattactt atgtctgcaa tatatagaag acaaaggagt
     6241 tgtacattat gattacaata agtttgtgca tgattgttaa gaatgaggaa gaagttatag
     6301 gcagatgctt agaatgtgta aaggatatag tagacgaaat tataattgta gatactggtt
     6361 ctacagattc cacaaaagaa atagtctcaa aatatacaga catggtgtac gatttcgaat
     6421 ggatagatga tttctctgct gcaagaaatt tttcattttc taaagcttct aaagactata
     6481 ctatgtggct tgatgcagat gacataattt tagaagctga tagagaaaaa ttattaaaga
     6541 ttaaagaaag cttggatact tccattgata ttgttatggc aaaatacaat gtatcctttg
     6601 atgaaaatgg taacccaact ctctcgtatt atagagaacg attgtttaaa agaagttcta
     6661 atcataaatg ggttgaccct attcatgaag tagtacctct ttttggagag gtattttatt
     6721 ctgacattgc aatatctcat aaaaagcttc atagacagga ccctctaaga aatcttagaa
     6781 tttttgaaaa aatgatttca gaaggaaaaa cattggagcc aagacaccaa ttctattatt
     6841 ctagagagtt gtactataat gctaggtata aagaagcaat agaaggtttt actaagtttt
     6901 tagactcatc aagaggatgg attgaggatt gtatcagtgc ttgcagagat ttagccacat
     6961 gttattactt aattaatgat gaaaaaagtg ccttatattc attgtttaga agtttcgaat
     7021 ttgatgagcc aagagcagaa atttgttgtg acataggaaa gcatatgttt gatagacaaa
     7081 aatataaaga agccattttt tggtataagg tagcattaac aagagataag aatgatacta
     7141 atggaggttt taagtcaaat gattgttatg gatatatacc atctatacaa ttaagtgtct
     7201 gttatgacag gttaggagaa tcagataaag ctatttatta tcatgaaaaa acaaaagaaa
     7261 tcaaacctaa cgatagtgct gttttgcaca atgaaaatta ttttagtaaa tttaaaaata
     7321 gttgataggt cacaatcata ctaaatttga catattatat atagagctta aaagctcaaa
     7381 tatatcagga ggtgctttta ataatgagta gaaataaata ttttggacca tttgatgata
     7441 atgattacaa caatggctat gataaatatg atgattgtaa caatggtcgt gatgattata
     7501 atagctgtga ttgccatcat tgctgtccac catcatgtgt aggtccaaca ggcccaatgg
     7561 gtccaagagg tagaaccggc ccaacaggtc caacgggtcc aacaggtcca ggagtagggg
     7621 caacaggccc aacaggacca accggtccga ctggcccaac aggaaataca gggaatacag
     7681 gagcaacagg attaagaggt ccaacaggag caacaggggc aacaggccca acaggagcga
     7741 caggagctat agggtttgga gtaacaggtc caacaggtcc aacaggagcg acaggagcaa
     7801 caggagcaga tggagtaaca ggtccaacag gtccaacggg agcaacagga gcagatggaa
     7861 taacaggtcc aacaggagca acaggggcaa caggatttgg agtaacaggt ccaacaggcc
     7921 caacaggagc aacaggagta ggagtaacag gagcaacagg attaataggt ccaacaggag
     7981 caacaggaac acctggagca acaggtccaa caggggcaat aggagcaaca ggaataggaa
     8041 taacaggtcc aacaggagca acaggagcaa caggggcaga tggagcaaca ggagtaacag
     8101 gcccaacagg cccaacaggg gcaacaggag cagatggagt aacaggccca acaggagcaa
     8161 caggagcaac aggaatagga ataacaggtc caacaggtcc aacaggagca acaggaatag
     8221 gaataacagg ggcaacagga ttaataggtc caacaggagc aacaggaaca cctggagcaa
     8281 caggtccaac aggagcaaca ggcccaacag gagtaggagt aacaggagca acaggagcaa
     8341 caggggcaac aggagcagac ggagcaacag gagtaacagg tccaacagga gcaacagggg
     8401 caacaggagc aaatggatta gtaggcccaa caggagcaac aggagcagca ggaacacctg
     8461 gagcaacagg tccaacagga gcaacagggg caacaggagt aggaataaca ggagcaacag
     8521 gggcaacagg agcgacaggt ccaacaggag cagatggagc aacaggtcca acaggagcaa
     8581 caggaaatac aggagcagat ggagtagcag gtccaacagg agcaacagga gcaacaggaa
     8641 atacaggagc agatggagca acaggtccaa caggagcaac aggaaataca ggagcagatg
     8701 gagcaacagg tccaacagga gcaacaggag caacaggggc agatggagca acaggtccaa
     8761 caggagcaac aggggcaaca ggagttacag gagcaacagg cccaacaggt gcaacaggag
     8821 caacaggagc aacaggtgct agtgcaataa taccttttgc atcaggtata ccactatcac
     8881 ttacaactat agctggagga ttagtaggta cacctggatt tgttggattt ggtagttcag
     8941 ctccaggatt aagtatagtt ggtggagtaa tagaccttac aaacgcagca ggaacattga
     9001 ctaactttgc attttcaatg ccaagagatg gaacaataac atctatttca gcatacttca
     9061 gtacaacagc agcactttca cttgttggtt caacaattac aattacagca acactttacc
     9121 aatctactgc accaaataac tcatttacag ctgtaccagg agcgacagtt acactagctc
     9181 caccacttac aggtatatta tcagttggtt caatttctag tggaattgta acaggattaa
     9241 atatagcagc aacagcgcaa actcgattct tactagtatt tactgcaaca gcttcaggtc
     9301 tttcattagt taatactgta gcaggatatg caagtgcagg aattgcaata aattagatta
     9361 gataagaaaa ggcttctaat atcgtattga tattaggagc ttttttaaga atgattgatg
     9421 aacatagttt taatattaat tagtgcgcat attataaaat actattatat aatataaaat
     9481 aataacatta aattggaaat atagaattat ctaggggtga tactatgaaa aataagattg
     9541 aaatatcaat gaacaacttt gtaaaaaatt atcataaatc aaataatata actacaaagt
     9601 ggcaagaact tttgtttgct tatgcatctg ctgaagatga tatgtttcag aagctaaaaa
     9661 gtgttgtagg aacatctcat gctatgccaa aagattttct ggaaaatgga aaaactataa
     9721 ttacatattt tatacctttt gatgaaagtg taaataatag taatattgga gggtttgaat
     9781 gttctgaaat atgggcaaga gcgtacatag aaactaatat gttgatttca aatttaaatg
     9841 atttcttatg tgaagaacta gagaagatag gggctaattc atatgctatt cctgggtcgt
     9901 ataattttga cagtgaaaaa ttggtaagtg actggtcaca aagacatgta gcattcatag
     9961 caggtcttgg aacttttggt ttaaataata tgcttattac agaaaaaggg tgttgtggaa
    10021 gaataggaag cataatcaca agtttagaat tagagccaac taaaagacca gaaaatgaat
    10081 actgcttgta taaacataat aatagttgca aaaaatgtgt caataaatgc gtgaaagaag
    10141 ctttaaaaga agatggattt aataggaata agtgttatga aatgtgtatg ataaatgaaa
    10201 aaaaatacac ttatataggt ggatgtgata tttgtggaaa gtgtatagtt ggagttcctt
    10261 gttcagtaat aaacccatca agataatgga cttaagttga ctaataattt taaagatagt
    10321 ttataatgaa aaaattttag ttatcaaaat aaatataata taaaaactaa ataaatgtgt
    10381 ttaaaaaatg gataaataat ttgtgttgat tgacaaccag ttatactgtt gatagactta
    10441 tatgtatcgc tgtgatatat aaggagggtt gagattttat gtttgattta atctttattt
    10501 ttgcatagca agggctattg caactatgaa atttaatata taaatagccc tttgctattc
    10561 ctaatgaaaa attactattt aataggagag caaaatgagc tataaaaaag caatacatat
    10621 attaccagaa gaattacttg aattaataca ggaatatgta gatggtgaat gtatctatat
    10681 tcctagaaaa tctaataata aaaaagaatg gggaagtaat acacctactc gtgaggagtt
    10741 gtcaattaga aatatgcaga tttacgaaga ctatcaagtt ggatatgatt tgcaatattt
    10801 atcggaaaag tattatttat ctttgaaaag tatccaaaga attgtattac aagaaaaaag
    10861 aaaagatatc taaggatgag ccagtatagt tttataatta tactggctca ttttcttttg
    10921 ataaaagtgt ttgaggtata gtttctttta agtggatgat ataatttaag taatttcaat
    10981 aacttataca gtagctaaca aagctggttg taaagtatct aataagaagt agtagctttt
    11041 tatatgtttg aaattaaaat ttattaaaat taggaggtct tgttttgaaa aaaatacttg
    11101 attatattcc accttttcat aactttctat taatttagta atcttataga aaggaaattg
    11161 catttatgaa aaaaattact tgggaggtgg aatatatctc ccagttacct gtaattaggt
    11221 attgtaaaaa atgtggaaaa aagactgaat atatttgttc aggtttattt agggttaatg
    11281 ctcaacataa atatttggat atatggttaa tatacaagtg ttcaaattgt gattcaacat
    11341 ggaactcaac tatatattct cgtgttaatc caaaaagatt aaatccagaa atactagaac
    11401 aattccatac aaatgattat aatcttgtta agcaatatgc tatgaatatg gaacttttac
    11461 atagaaatgg agcagaagtt ggtattccag agtataaaat agtaggacaa gaaattaata
    11521 ttaatgacct tacacaagtt catattatta gtaaatatcc atgtcagtta aaggtatcaa
    11581 cattattacg tgagaaacta ggtttatcta ggaaggtatt tgacgaaatg ttggattgtg
    11641 ggattattag aagtaactca ggagttgatt taaagaaagg taaattaaat tatgaaatta
    11701 tgttagatat aggcaaggag aagcaaaagt cttcaaattg attagaataa ctaacttaat
    11761 atattttcag gaacatcatt taaaaaaata gtgactttaa aaaatttaaa gccactattt
    11821 tttaatatgt tttatatgag tatttaaaat aaagtgtgta ttattcaaat ttatagcgat
    11881 aattaaaaca ttactataaa aaggaaggtg catacttttt atttaacaaa aactagatat
    11941 taaaaacttt tcgaggaata ttttattaaa atccctaaat atatttaata tatattaaaa
    12001 aattgtatta taatgaaaat caaaatagat tgcaaatcca aaaagtaata cttaatgtcc
    12061 aaaaagctaa aattaaaatc taaatagcta tttactacag aagtatgtta aaatatgtaa
    12121 aatgaataga aatttatata tttttattaa aaatatataa gaaaggatgt ttaagaattg
    12181 tgattaatat aggaatatgt gatgatgaac tacattatcg aataaaaata aaagatatat
    12241 taagtgaaat attaagttca tatcctataa attataacat atatgaattt tcagcaggag
    12301 aagagttact aaataactat cctaaagatt tagacatatt aattatggac atacaaatga
    12361 aaacaataaa tggtatggat actgctagga agataagaga gtttgatcat aagttagaaa
    12421 taatatttgt tacttcattt gtagagttta tgcaagaagg ttatgaggta aaagcataca
    12481 gatatatact aaagccaata aataaagaaa aaatttctaa aagtgtatta ccttgtataa
    12541 atgagatgat gaaaaagaga aataattact taactataaa tgtaaaaaat tatgtagacc
    12601 gaattaaaat agattctatt acttatatag aaacagatag accgaatata ttgatttata
    12661 cacatgatga tatgtatatt actaaaatga gtatatctaa aatagaaaag atattaaatg
    12721 aatatggatt ttttagatgt cataatagct atatagttaa cttaaaatta gtagaatcaa
    12781 tgagtggaag tactgttata gtagatggaa aaagtattcc aataagtaaa tatagggtta
    12841 aaggcttgaa gcttgcgata actaatatat taggggacat agtatgctaa caaatttaag
    12901 tgtaggattt tggaatatta ttaatttagt ttcaggtact atagattgga ttatattttt
    12961 tttagtaatc cacttagttg gaaagaggaa aatatctaat ataagatata tatttacaac
    13021 aataattata attatattta tggcatacat aaatgtagtt aaagtgtttc caaatattaa
    13081 gattcttcta tgtatggtaa taggaatatc attttatata acatcatatg aagataaaat
    13141 ctataaaagt ataatagtta gtttattgtt ttggctagga ttaatggttg ctgaaggttt
    13201 tgcagtaggt ctagtagtat ttataaataa attaaataat attaacataa cacaacatga
    13261 aaacttattt agaatacaag ctattattat gtcaaaggta tttttattta taggttttat
    13321 attgtttaag tattttaaat tatctttaga ttttaaacct aaagatatga tactaatagg
    13381 aatacctata ttatctaata tagttagctt attacttata tttgaatata atttaagagt
    13441 caatacaatt actacagaaa acttatttat atttatattt gcaattttat tgattttatc
    13501 atcaagtata atacttttaa tagtaatagg gaaaattgta caagacgata aattaaatct
    13561 agagtatgaa cttataaatg aaagaataaa aacaaactac aggaattatg aaaatataaa
    13621 cgaagttcat gatagattaa aatatgttta tcatgacttg aaaaaccata tgatttgtat
    13681 aaaaaattat gacactaaag aagaaattat atcatatata aataacttag agtttcagat
    13741 aagtgaattt gaacatttta aaaatactgg aaataaaaca ttagatttaa ttttagcaga
    13801 aaaaataagt atttgtaaaa agtatgatat tcaaattgaa gataacataa atatatctaa
    13861 attaaacttt attaaaaata atgatatatg ttcaatattt gcaaattcac tagataatgc
    13921 aattgaagca tgtatggata taaataatga gatagaaaaa agaatagaag ttaaagctac
    13981 atatataaat aaatttgcaa taataaaatt tataaatact aaggtaaatg atattaaact
    14041 tattgataaa cgtattcaaa ctagtaagga tgatgataag atacatggaa taggacttgc
    14101 tagtattaaa tatatagtaa ataaatatgg tggtgaaaca atagttaatt actcagataa
    14161 tgagtttata ttaaaaataa tgataccaat aaaaagttag gagagtacaa aatgaaaaaa
    14221 atagcgttaa acttacttaa aaacatctca gcattaagtt ttgggatagc tgtattatca
    14281 gcaaacagtg caagttcttg ggtagcacat caggcaaaag agccacaagc tttacaaaaa
    14341 ctaaaaaaat aacattaata aaaggacctc ttcttattaa aggagaggtt ttacatgttg
    14401 aggaggaaat atgtttaaaa gattatcata taaatttgcc aatatattag ttaataatga
    14461 gattgtagaa agtgaagatt ttgaaatata tagatatgga tttgaaactt tgatttattt
    14521 catagttaat attttggtag cattatttat aggaattatt tttgatagat ttatacatac
    14581 agttatattt ttaagttgtt attgcacttt aagacagttc acaggaggtt atcatgctag
    14641 gaattataaa gagtgtacat taacatttgc agttatttat ttgataacta ttttttcagc
    14701 taataatata gatataaata aatataagta tttattagta ttattaatga ttattagtat
    14761 actaacaatt tacaagttag ctcctttaga acatagaaac aaacctctta gtgaaagtga
    14821 gaaaaagcat tatagaaaga ctgtgcaaaa aatactattc gtaataatat gtttaattat
    14881 tttatgtaaa atacttaata tttttcagca atatgtgatt tatgcaataa tttctatata
    14941 ttggattgct atattaatat atattggaat gaaggttaat agtgatcagt aagattataa
    15001 aacattttct aaattaatta acttcactaa ttaatattaa ctttaaaaaa ataatgtcaa
    15061 ggtaagtatt tgaacttatt aaatatagtt gaatctattg tgggttgtat caaaagactg
    15121 gctaggaggg gcataaatat gagtagtatt aaggttatat cagactttat aaccaaaata
    15181 attagaaaaa atattttatt tgcaataaca gttattatta taaattacca tctcatacaa
    15241 cgatggacac atatatccaa tagaggaatt tcatttaatg attacttatc cttgagtgta
    15301 tatacttgta tgacaattag tattttaacc atgttaaatg taatttttta tatgagaaaa
    15361 agttatgata ctgtaaaaat attatgtaga aataattttg attatttttt aggtatttct
    15421 gttgctgtaa ctagaaacaa ctttatattt tgttgcatac caattatata tgctatagca
    15481 tactcaagtt taataagaac attgtcaatt gtaacgatta aatcaactat atttatattg
    15541 atatttgaat ggtttttacc acttactatt ttatctatag tgatatcatt tatttcagtt
    15601 attataaata atttaatact aaagattacc ttatcatcag tatttttata tttaacatca
    15661 actaatatgt tggatagagt tattgatatt aaagaaccat tttttaaagc attatttaaa
    15721 tcacttaaca tatttgaaga tcaatcatat gcttactatt ctgattttgt agggaaagtt
    15781 aataattcag catatgttat agataagatt atacctacac tatttgcaat tactttaata
    15841 atagtagcat atctgatatt aaatgatatt agaaagaaac agattaaatc attaatagtt
    15901 atagtagtgt acagtatttt atttattgga ttaaattata tatcaataga tatgtcaaca
    15961 tatagagatg aggagcgtag ttatggagag tatttaaaga aatctaatat tagtgatatt
    16021 aaaataaagt cgcatgatat gaagattgac tttaaaagca agtcaaatat aaaagataga
    16081 attgttttat taaacacatc aaatgaagag ttaacaaata ttaatatgat tttagatgaa
    16141 atatttgata taaaatatat aaaagtaaat tcggaaaata taaaatttaa cataaaaaag
    16201 gatttaattg aagttaattt aaaggaaagt ttagagccta atgacgaggt gacattagat
    16261 atagagtatg aaggctatat taatgtgttg acatcttgga atcaaaataa atatatagct
    16321 actaaagatg atattatgct tccaccaggt tctttagcgt ggtacccaaa agtaaatcaa
    16381 tcaagttata tggcttataa aataaattta agttctaaaa ataatgtcta ttcaaattta
    16441 cctttactga ataagactag taatttttta aaaaaagatt atatatttga aggaaagtca
    16501 aatgatattg caatatatgc aggtgagttt aaagaagagg taaaagatga tataaaaata
    16561 atctatccat cagatagtaa tgtaaatatg gatatagagg aaaaaattaa atttttaatt
    16621 gaaacaagaa atgaaatatt aggtgatagc aaagcatctg tagatgctaa aaaatatgta
    16681 gatgaagtag ataatgttat ggaatcaata aaaagaaaaa aaattaatat gatattagtt
    16741 gctgacttac ctagcaatga atttttatat gaagactcaa agggagagaa aattagcata
    16801 actatttatc attggctttc aggaaatatc cttataatag ataaataata aaggaggtta
    16861 atatgataaa caagttagaa ataagaaatc taaattttaa ttacaaaaat aaaaaagctc
    16921 tggataatat aaatttaacc ctaacagatg gagtagttgc attattaggt cctaatggtg
    16981 ctggaaaatc aacattaatg aggttattag tgactcttta tgaaacttca attggagaga
    17041 tagaattaaa taatataaag tatagcaaaa acaatgagaa aatcaaggca aatgtaggat
    17101 atgtacctca agattttgat atgtataata atataaatgg gcaagaatac ttagaatttg
    17161 tagctaaaat gagagatgta tctaagaatg atttaaagaa acatatacaa aaagtagtat
    17221 caaaagttaa tctggacaag tttataaata aaaaaatagg tacatactca ggtggtgtaa
    17281 agagacgact aggtatagca caggctttaa taggtgattc taagttaata gttatggacg
    17341 aacctacagt tggtcttgat ccagagcaaa ggaatgaatt tagaagattg ttaccagaaa
    17401 taagtaaaaa ttctatagta ttaatttcaa ctcacatagt agaagacata caatttaact
    17461 gtgacaaatt aattatatta aatcaaggca aaatattata tgatggtaca attaataagt
    17521 ttattgatat ggttgaggtg tattcggtag tagtttctaa tgatgaattt gaatatttag
    17581 aacgtaatat tgatattatt gattatagga gagttaaaga tggtgtgcaa gtaaagtata
    17641 taggtaaaaa tacagagaaa cttaaagata gcttaaggat agatacaact ttacaagatg
    17701 cttatataaa ctttttacat aaggagttaa aataaatgtg tatattggag ttaataaaat
    17761 ttgagcttaa aaaaatagag cttaaacatg tgttaatatt tgtaattatt tctatattat
    17821 gcatatttat tacaggcata ttagattata atgagttaat attttataat gatgtaccta
    17881 taatggatac atattctata gcaggatggt taggaattat aaagatatca gatgttttta
    17941 taccaataat gtcagcaatc acaataattt atgtgttttc aaaagattat tcgggtaata
    18001 taaatgagat acttacttta tataataaaa aaaaatataa tcaatttata gtatttagat
    18061 ggtgtgttat attagctata tatactgtat tattattaat ttcaattaca atagtatata
    18121 catttacgtc agtgaaaggt tttaacatag acaaaataga ttttgtttat agtgtaaagc
    18181 caatagatgt gtttttaaag acgtttccta cattattatg gtatacaaca tttccactaa
    18241 tgatattaac attaacaaaa aatagattta caacaatagc aatattaatt acatatacat
    18301 ttgctgatat attttttatt ttacatatgt acccatttgt tagtatgatt aatgtaaatg
    18361 gattttatat tcaaaagatg ttttatatgc ctaacaatat agatgttaga tttaatgaat
    18421 taaagaatta tttctttata aatagaggtt ctctagtagt tatttctata ataatagttt
    18481 tttatatagc aagaaaatca acggttttaa aaaagagtaa atagtttaat atatttttta
    18541 tattgttata tttatataga atagtgactt taaaaaattt aaagccacta ttttttaata
    18601 tgttttatct atataactat tatttttcta ggttttttaa tatttcgtaa tagtgtgttg
    18661 tggctatttc acttgactcg ccttttttac aaaaagataa tttggatagt tttggaacta
    18721 cacgttcaac gacaatttta ttacctatca ttttagattc aagtatcttc aatgcttcta
    18781 aaaatctatt atcctttttt gcacgattat aaaatgataa aacgtagaca taaaggaata
    18841 ggttataatt acggaaagga tattcaactt gcataaataa tgtgccaatt ccatagtgac
    18901 aagggccgat tggtttttta atagtccaat ggtcaagtag aaaatctaca gctttgtcaa
    18961 gttttgattc tttatttaga taattactaa aacggaatgc atttaatata ttaagagttg
    19021 gtagtggatt tgagtattca gtttctggac ctcgaccaaa actaaattta ttgcaacgcc
    19081 acccaccatc agtatactgt gtatctaaaa aatgttgaaa cgttttgtgc attctaatgt
    19141 cagatgcata tcccatatga cagagtacgt tagcagcatt tgcagtatga caagggtaaa
    19201 tacctccatt tggatatagt ttgaaacgtc catcttctcg ccatgtgcta aatatcaagt
    19261 cagcacattc tttaagtaaa gtatcatcag ggttcacacc taactctaat agataaagta
    19321 cactatcaag agtagagaaa ggagaacctt ttattaaacg cttatcaggt gtagtccaat
    19381 agtctgcacc attatcatac ctgtgtgata atattatttc aatatctgat aaagattgat
    19441 tttctacatt cattttttac cccatttcat attatattct attaaatcta tcaaaaactt
    19501 tattataata ttatttatat ttataattga tttattttat ttaaaattgc atttatagct
    19561 gtatcaggaa caactgctaa atcttctgca ttgaagtaat aaactatatt agaatcaaat
    19621 aatatatttg aatttggagg aaattcatca tctccaaacc aaagtataaa tatcatagat
    19681 gtattaccca tatatgtaaa cctataagct aaatctccca tgtttacttt ttcagctcca
    19741 attttctcca taactaactt atatttgtct atttgagatg taaaaacttc tgaaagccta
    19801 tatagagtcc tgtttaaaaa atttggataa taaacattgc ctcctggtat ttctttaaaa
    19861 gttacatatt tattagttct aggaatacct ttagcattga ttaaatatct aactatcaag
    19921 attttaagct caaagttatt gcaaaggcta ccattactat tccaaacttc tccacttgga
    19981 tagtctataa tatattgtct atcaaaaaaa ttgagtgtga atctatttaa atcattattg
    20041 aaaggtatat tacttaaatt actaattgta tgagggtcta attctttgag t
//
LOCUS       DAELJO010000057        19804 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELJO010000057 DAELJO010000000
VERSION     DAELJO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.057"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..195
                     /locus_tag="KPB88_001744"
     CDS             <1..195
                     /locus_tag="KPB88_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054026.1"
                     /translation="SRAMSELRSLIKQYDEMIHKDWNLATEEQRARVEVLKSKINNQE
                     SKEDKLDRYFDKLESVIKND"
     gene            188..1417
                     /locus_tag="KPB88_001745"
     CDS             188..1417
                     /locus_tag="KPB88_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0054027.1"
                     /translation="MIDELYHSKQLEVLNFALNNDYFMLINYGAKRTGKTIIDNDLFL
                     LELRRVRKIANELGIKLPQYILAGADLGALQRNVLSELTNKYDIEFKFDKHNRFVLFG
                     VQVCCFGHSKTNDLGRIRGMTSFGAYINEGTVANEEVFNEIKSRCSGEGARILVDTNP
                     DQPEHWLKTNFVDKTDGKVIQSFHYKLDDNIFLSERYRANIKKSTPSGVFYDRDINGL
                     WVSADGLVYQDFNKDIHYISKDKLNDINFVRYFAGVDWGYEHFGAIVVIGEDDKGNLY
                     LLEEHSAQHKEIDYWIDKAKSIKEKYGNIKFYCDSARPEHLAAFKRNGIKAFNANKAV
                     LSGVEAVAKRIKTNTLFVVHENVNLFRKEIFMYAWNKNTGEPIKKWDDVLDALRYAIY
                     TDSLGTGIKVLTPNGRR"
     gene            1422..2816
                     /locus_tag="KPB88_001746"
     CDS             1422..2816
                     /locus_tag="KPB88_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0054028.1"
                     /translation="MELDVIKKLIEQTNSKHSNFVKKADEAEKYYKNENDIIRDRSPN
                     NIGKVNTTNNPLRNADNRIPFNWFGFLVNQKISYLFTYPPTFDVGDDRVNSKITDILG
                     DRYPKEAKTLGKNASIYSKAWLHIWVDDNNNFQYANIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDKGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NVYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICRHLGYSPKR
                     VLQTWTRNMIQNDLELAEICSKSVGIISEKTNLKNHPLVDNAEEEEKQIKKENSQQEY
                     DDLIPNQDGGIDET"
     gene            2806..4341
                     /locus_tag="KPB88_001747"
     CDS             2806..4341
                     /locus_tag="KPB88_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0054029.1"
                     /translation="MKHNDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEARKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCHCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            4343..4591
                     /locus_tag="KPB88_001748"
     CDS             4343..4591
                     /locus_tag="KPB88_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054030.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            4662..4907
                     /locus_tag="KPB88_001749"
     CDS             4662..4907
                     /locus_tag="KPB88_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054031.1"
                     /translation="MLKLYILSIIVFCTGLYLFKMKIDSNEELIEILKNKKVRRKCNF
                     IFMALFPLLNFILGVIFIISSLLVSNEDMIKNLKGDK"
     gene            4909..5445
                     /locus_tag="KPB88_001750"
     CDS             4909..5445
                     /locus_tag="KPB88_001750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054032.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     MNRKGEVKKTICIKYKNGVIRATNGDFIIKDSEGKIYSCTFSEFEKLYENLERGHRYV
                     DEDNNKKNKLELSAKLELDTTDFEENIKSATKEIETLNEVVDRLEKKLNRIFAKENKV
                     DIDSIVKQLEERLRESIE"
     gene            5464..5736
                     /locus_tag="KPB88_001751"
     CDS             5464..5736
                     /locus_tag="KPB88_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054033.1"
                     /translation="MFKKKYIKKPSKISVRNIMAFIITVVGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLAGVISGYLIGKAIYLIASLISHIIYE"
     gene            5787..6014
                     /locus_tag="KPB88_001752"
     CDS             5787..6014
                     /locus_tag="KPB88_001752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054034.1"
                     /translation="MKEYVIWFKSGNCVSGITNEDVTDKLMKDFMEADSDCRYLKGYL
                     DEDGTTVIDLSQIEAISINNCSENNIGFSKS"
     gene            6060..6632
                     /locus_tag="KPB88_001753"
     CDS             6060..6632
                     /locus_tag="KPB88_001753"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0054035.1"
                     /translation="MKKGELIALGLSEEDAKKVEAESLKELENYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQKKLKEKEQKEFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDTWLTQKSFLIKTGETKINYSPDNGN
                     KNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            6648..7028
                     /locus_tag="KPB88_001754"
     CDS             6648..7028
                     /locus_tag="KPB88_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054036.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVIIE
                     RLQGEYVKEAIETIKTKLPNIKFM"
     gene            7040..8059
                     /locus_tag="KPB88_001755"
     CDS             7040..8059
                     /locus_tag="KPB88_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG0054037.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEEPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNIILRDEKLRKAIFGVNS
                     DKLLTLNELNTFLVSQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPAIDISSVGNIIVEQYSTADPVAKWIKAVATALPSFPYADQVF
                     MGTIN"
     gene            8070..8414
                     /locus_tag="KPB88_001756"
     CDS             8070..8414
                     /locus_tag="KPB88_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG0054038.1"
                     /translation="MEVERLKKLLGISKEDYSKEMILEFILEDVEEIVKNYCNVSVIP
                     EGLNSTVLRMAIDMYKNENLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKIRWK"
     gene            8414..8776
                     /locus_tag="KPB88_001757"
     CDS             8414..8776
                     /locus_tag="KPB88_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054039.1"
                     /translation="MMDKTRKAIEMLYRDKCTIIEYQPVKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNINSTEEGKVAKLAQTIKLFISPNISVKAGSKLIITTQNNITREYVRSGES
                     AIYPNHQEIILELFKDKA"
     gene            8776..9201
                     /locus_tag="KPB88_001758"
     CDS             8776..9201
                     /locus_tag="KPB88_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0054040.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIERKLLILLKGVFDA"
     gene            9194..9631
                     /locus_tag="KPB88_001759"
     CDS             9194..9631
                     /locus_tag="KPB88_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054041.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPRSKDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTEKMNDVELFGGLKRGDNFE"
     gene            9624..9800
                     /locus_tag="KPB88_001760"
     CDS             9624..9800
                     /locus_tag="KPB88_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054042.1"
                     /translation="MNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEEIINNFMKGVS"
     gene            9801..11111
                     /locus_tag="KPB88_001761"
     CDS             9801..11111
                     /locus_tag="KPB88_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG0054043.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIKLGYFYKLNKGIKAS
                     CSIATAKYSGIRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYV
                     IWKKDATLEASAGLVFTGGTNGEAVTGAEYQTFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWVTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     GKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            11133..11603
                     /locus_tag="KPB88_001762"
     CDS             11133..11603
                     /locus_tag="KPB88_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0054044.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            11656..11913
                     /locus_tag="KPB88_001763"
     CDS             11656..11913
                     /locus_tag="KPB88_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054045.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            11918..12358
                     /locus_tag="KPB88_001764"
     CDS             11918..12358
                     /locus_tag="KPB88_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0054046.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            12679..12840
                     /locus_tag="KPB88_001765"
     CDS             12679..12840
                     /locus_tag="KPB88_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054047.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            12989..13222
                     /locus_tag="KPB88_001766"
     CDS             12989..13222
                     /locus_tag="KPB88_001766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054048.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKYYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            13282..13809
                     /locus_tag="KPB88_001767"
     CDS             13282..13809
                     /locus_tag="KPB88_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0054049.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            13951..14511
                     /locus_tag="KPB88_001768"
     CDS             13951..14511
                     /locus_tag="KPB88_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0054050.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            14589..16877
                     /locus_tag="KPB88_001769"
     CDS             14589..16877
                     /locus_tag="KPB88_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG0054051.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDNDLD
                     GIVNVLTEKLNDAMAVSAEGIV"
     gene            16894..17595
                     /locus_tag="KPB88_001770"
     CDS             16894..17595
                     /locus_tag="KPB88_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0054052.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     LSKYTIYKGQVLRLV"
     gene            17588..19552
                     /locus_tag="KPB88_001771"
     CDS             17588..19552
                     /locus_tag="KPB88_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0054053.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGNIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFSSVDEGIKKGISNLSRNYIHTG
                     RKTLERIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSTYTSNNAGTGVGSNEEAEKNL
                     KDTIYQVQNNNSNTSTNNNSKVSKVIQEAKNQLGKPYAWGGNGPKSFDCSGLMVWAFK
                     RGAGINLPRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYIH
                     APQTGDVVKISSLSGRQKKKRDFARARRFF"
     gene            19566..>19804
                     /locus_tag="KPB88_001772"
     CDS             19566..>19804
                     /locus_tag="KPB88_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0054054.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPIQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKKYTDKKIGDKLVLIRMQGGQQ"
BASE COUNT         7963 a         2105 c         3860 g         5876 t
ORIGIN      
        1 agtagggcaa tgagtgagtt aaggagttta attaagcagt atgatgagat gattcataag
       61 gattggaatt tagctacaga ggagcaaaga gcaagagtag aagtattaaa atcaaagata
      121 aataatcaag aaagtaaaga agataaactt gatagatatt ttgataaact cgaaagtgtg
      181 ataaaaaatg attgatgaat tatatcatag taaacaactt gaagttctta actttgcttt
      241 gaataatgac tacttcatgc taattaatta tggtgctaaa agaacaggaa aaacaataat
      301 tgacaatgat ttattcttac ttgaacttag aagggttaga aagatagcta atgagctagg
      361 tattaagctt cctcaataca tcttagcagg agcagattta ggagctttac aaagaaatgt
      421 actaagtgag cttacaaata agtatgacat tgagtttaag tttgacaaac ataatagatt
      481 tgtattgttt ggagttcaag tatgttgctt tggacactca aaaacaaatg atttaggcag
      541 aataagaggt atgacttcgt ttggggcata cataaatgaa ggtacagttg caaatgaaga
      601 agtatttaat gaaatcaaat caagatgtag tggagaaggt gctagaatac ttgtagatac
      661 taacccagac caaccagagc attggttaaa aactaacttt gtagataaaa cagatggcaa
      721 ggttattcaa tcttttcatt acaaattaga tgataatata tttttaagtg aaagatacag
      781 agcaaatatt aagaagtcta ctcctagtgg cgttttttat gacagagata taaatggttt
      841 atgggtgtca gcagatggat tagtttatca agattttaat aaggatattc attatatttc
      901 taaggataaa ttaaatgata ttaattttgt aagatacttt gcaggcgttg attggggata
      961 tgaacatttt ggagctattg ttgtaattgg agaagatgat aaaggtaact tatatttatt
     1021 agaagaacat tcagctcaac ataaggaaat agattactgg atagataagg ctaaaagtat
     1081 aaaagaaaag tatggaaata taaagtttta ttgtgatagt gctagacccg aacatttggc
     1141 agcatttaaa agaaatggta taaaagcttt taatgctaat aaagctgtat tgtctggcgt
     1201 tgaagctgta gctaaaagaa ttaaaactaa tactttattt gtagttcatg agaatgttaa
     1261 tttattcagg aaagaaattt ttatgtatgc ttggaataaa aatacaggtg agccaattaa
     1321 aaagtgggat gatgtattag acgcacttag atatgccatc tatacagatt ccttaggaac
     1381 aggaattaaa gtacttacac caaatggaag aaggtgataa attggaatta gatgtaataa
     1441 aaaagttaat tgaacaaact aatagtaaac atagtaactt tgttaaaaaa gctgatgaag
     1501 ctgaaaaata ctataaaaat gaaaatgaca ttataaggga tagaagccct aataatattg
     1561 gaaaagtaaa tacaactaat aatccactaa gaaatgcaga taatagaata ccatttaatt
     1621 ggtttggttt tttagttaac caaaaaatat catatctgtt tacttatcca cctacatttg
     1681 atgttggaga tgatagagta aattcaaaga taactgatat tttaggcgat agatacccaa
     1741 aagaagctaa aacacttggc aaaaatgcta gtatatattc taaagcatgg ttacatattt
     1801 gggtggatga taataataac tttcaatatg ctaatataga ccctcgccaa ataagagcag
     1861 tatattcatc agatttaaac agaaagcttc ttgcagtact tagagaatat aaaaagactg
     1921 atgataaagg aaaagaatat gtaatttatg agtattggac agatgaatgt tgttatactt
     1981 atcaaaataa agatggaaat agtaatatca atggattgga gatacttaat aaatttatag
     2041 agaaaaattt agataataaa cttgaaactc aaactaatgt atataaacac aattttggag
     2101 aagttccatt tatcgaattt ctaaacaatg atttagaggt aagagattta gataatgtta
     2161 aacacctcat tgatgtatat gacaaggttt atagcggttt tgtgaatgat attgaagata
     2221 tacaagaagt tattttcgtt cttacgaact atggaggtgc agacttaaca gagttcttaa
     2281 aaggacttaa agaatataaa actattgatt tacaaagtag tggtgcagat gataagagtg
     2341 ggttaagtac aattacaata aatattccaa ttgaagctcg agattcactt cttaaaacaa
     2401 cagaaaagca aatctatgtc caaggtcaag gtgttgaccc taaacccgaa aactttgcca
     2461 atacaagtgg tgtagcactc aaattcttgt acactttgtt agaattaaaa gcaggactta
     2521 tggaaacaga gtttagatta gggtttgcta aactagtaag aatgatatgt agacatttag
     2581 gatattctcc taaaagggtt ttgcaaactt ggactaggaa tatgattcaa aatgatttag
     2641 aactagctga gatatgctct aagagtgttg gaataatatc agaaaagact aatttaaaaa
     2701 atcatccgct tgtagataat gcagaggaag aagaaaaaca gattaaaaaa gaaaatagcc
     2761 aacaagaata tgatgattta attcctaatc aagacggtgg catagatgaa acataatgat
     2821 tattggagaa agagatttga acaattagaa gaagctcaaa ataacaaaag tgtaaaatat
     2881 tatcttgaat tagaaaagca atataaacta gctataaata gtatagaaaa agatatatta
     2941 gcatggtaca acagatttgc caaaaatgaa ggaatatctt tattagaagc taggaaacta
     3001 ctaaatacaa gagaactaga agagtttaaa tggagtgtag aagaatatat taaatatggt
     3061 aaagaaaatg ctataaatca aaagtggatg aaagagttag aaaatgctag tgcaagagtt
     3121 catataacaa ggcttgaagc tttaaagtta caaatacagc aacaagtgga agtgctttat
     3181 ggaaatgaac ttgatggtat tgataaatta atgagacata tttatacaag tggatattat
     3241 catgcagctt ttaatgttca acaaggagta aacgttggtt ggagtttaat gaatcttgat
     3301 actaatagaa taaataaagt tatctctaaa ccatggacta gtgatggatt aaattttagt
     3361 gaaaggattt ggggcaaaca tagacctgct ttaattaatg agttacatac taagctaact
     3421 caatcaatta ttagaggtga aaatccaaag aacctagtaa atgacttttc taagagattt
     3481 aatgtatcta aatcacaagc taagaatttg ataatgactg aatcagcttt ctttgcatca
     3541 gcttcaagaa aagattgttt caatgattta gatgtagaga agtatgagat tattgccaca
     3601 ctagacttaa aaacatcaaa tatatgtaga gagttagatg gaaaagtatt tgatatgaag
     3661 gattatcaag ttggagttac agctccacca tttcattgtc attgtaggac aacaacagct
     3721 ccttggtttg aagatgaaga aggttacaga gcagcaaaag gagaagatgg aaaaacatat
     3781 tatgtaccat ctaatatgaa gtataatgag tggtatgaga aacatataga gggtagactt
     3841 ggaaaagaaa aagctgatac tcttagaaaa atgcaactaa atgagagtaa agatagaaaa
     3901 caatttgaag aatataaaaa ggtattagga aatgaaatac cttctaaatt tgatgaatat
     3961 caacatatga agtataatga tactataagg tatgaggaag ctaaaaagct ttataaggat
     4021 gtaaattggc aagttaagaa tcagagaaat ttaactagtg gaagtgttca ttcagtgcca
     4081 tttgaatcaa aaccaaatag tgtatttgat aattacaaag atggtaaatt aattcaaaga
     4141 agatactatg gtaatacagg aaaacctaga ttagatttag acttaactga ccatagaaac
     4201 ccaaagcaac ataaaattgt acctcataaa catgattggt tagctgatga aaataaccat
     4261 gataaggtaa aaagagagaa ggatatggag cttacaaaag ctcataaaat agcaaacaaa
     4321 gatattttga aaggagagta aaatggataa taaatttaag aatttacaac atttaattga
     4381 ttctattgat ttaggattag atatagaatt tgacttatat aataaaccat ataatatatc
     4441 tattggagat gatgatacaa ggtttattac tctttgccct aatggagata caaagtatta
     4501 taaaaatggt aaggatatgg ttgataatta caacattgat ggtaaattac taaaggattt
     4561 atggaaagat atacaaatag taaatatgta aaagcacttg ctaagttata aattagtagg
     4621 tgcttttatt atgtaaaaat ttattgagag ggtgatttga aatgcttaaa ttatatattt
     4681 tatcaataat tgtgttttgt acagggcttt atttatttaa aatgaagatt gatagtaatg
     4741 aggaattgat tgagattctt aaaaataaaa aagtgagaag aaagtgtaat tttattttta
     4801 tggctttatt tccactactt aattttattt taggtgtgat atttatcata tcttctttac
     4861 tagttagcaa tgaagatatg attaaaaatt taaaggggga taaataatat ggctaaattt
     4921 aaaaagaaat cagaggaagt agaagctttt aaatggatat taggaagtcc taatactcct
     4981 atatggtttt atcaagcttt tgagaaggga actatatggc ttgatgaatc tttaaactct
     5041 atgaatcgta aaggtgaagt aaagaaaact atctgtataa aatataaaaa tggagttatt
     5101 agggcaacta atggagattt tattataaaa gatagtgaag gcaagattta ttcatgtaca
     5161 tttagtgaat ttgagaagtt atatgaaaat ttagaaagag gacatagata tgttgatgaa
     5221 gataacaaca agaaaaataa attagaactt tcagctaaat tggaactaga tacaactgat
     5281 tttgaggaaa atataaaaag tgctacaaaa gaaattgaaa cactcaatga agtagtagat
     5341 agattagaga aaaaattaaa tagaatattt gcaaaagaaa ataaagttga tatagatagt
     5401 attgtaaaac aattagagga acgtctaaga gaaagtattg aataagtttt gagggatgga
     5461 aatatgttta aaaagaaata tattaaaaag ccaagcaaaa taagtgttag aaatattatg
     5521 gcatttatta ttacagttgt tggtatagct ttaggtgttt ttataggtat caacataatc
     5581 atggctcatg ttttaggtat agctaacatg gtagataata atacttttac atgtgttaga
     5641 ttagtttatg gcttagcagg agttataagt gggtatttga taggaaaagc aatatacctt
     5701 atagcttcgc taataagcca tattatttat gaataatttg ttcaaagatt ttttattttg
     5761 taagaaatga aaggagggtt gtattattga aagagtatgt aatttggttt aagagtggaa
     5821 attgtgtatc tggaataaca aacgaagatg ttactgataa gttaatgaaa gattttatgg
     5881 aagctgactc agattgtagg tatttgaaag ggtatttaga tgaagatgga acaactgtaa
     5941 tagatttatc acaaatagaa gctatatcaa taaataattg tagtgagaat aatattggtt
     6001 ttagtaagtc ctaacaaggg cttttttatt ttctaaaaat gaaaggagaa ttaaataaaa
     6061 tgaaaaaagg tgaactaatt gcactaggtc ttagtgaaga agatgcaaag aaagtagaag
     6121 ctgaatcatt aaaagaatta gaaaattata ttaataaaat tgagtatgaa aaggtaaaag
     6181 aggaactaaa agcctctaaa gaagcaattg aaggttttaa ggatggaatg acaaaagagc
     6241 agattgaaga gcttaaaaaa ggctatgaga ctaaattaac tgcaaaagat gaagaatatc
     6301 aaaagaaatt aaaggaaaaa gaacaaaaag agtttgatat ggcgttagaa aatgaactta
     6361 ttaaacttaa tgttcatagc actaaagcag caaaagcaga acttgattta gaaaaaataa
     6421 aatatgaaaa tggtactttt acaggactaa aggaacagac tgatacttgg ttaactcaaa
     6481 aatctttctt aataaaaaca ggagagacta agataaatta cagccccgat aatggcaata
     6541 aaaatacatt aagtagagct gaaaatattg ctaaagaaaa gaatgaggaa ggttctaaaa
     6601 atccatatgc tgacgcatgg agtataaaat aaaaaggagg ataaagtatg tattttaaag
     6661 aggtaaattt tgataacaca cccgagtttc tagcttctca acactatatt aacttttcaa
     6721 aaacagcatt agatacagat gtagtggctg atgaaaatgg aaagaaatat gtgttagcag
     6781 gtagtttatt aggtgagagt ggcaaagtgg taaagataac cagaggaggt tcttcgggta
     6841 gttatacata cacattatca gaagaccctg taggaatagt tttttcaact gtagatgtta
     6901 cttatggacc acaaccagtt gcatcaatgg tggaaggcta tgtaataatc gaaagattgc
     6961 aaggtgagta tgtaaaagaa gctatagaaa ctataaagac aaaattacca aatattaaat
     7021 ttatgtagga ggatgaaata tggcaagagt agaagaatta ttgtcagttc aagagctgat
     7081 aaactataca aagactagaa aattaaaaga aacaatggga gatttattat ttccaactca
     7141 aaagatagaa ggacttgaaa taaagatgat aaaaggtgca tctaatcttc cagtatcagc
     7201 aagtgttcat gcttttgata cagaagcaga gattgcatca agagaaggtg ctaatttaag
     7261 tattgctgaa cttgcacttg tgaaaagaaa aattaaacta gatgaaaaag atataattgt
     7321 acttgaagag ccaagaaact cacaagaaga aactcaaatg ataaatcaaa tatttaatga
     7381 tgttgataac cttgtatcaa gtgtaaatac tagaatagaa gcaatgagaa tggaagtttt
     7441 atcaactgga gaacttaata taaatgaaaa tggagttaaa gcttctttaa aatatggaac
     7501 tccaacaaat cataaagaaa caaaaacttg gtctagtgga acaccagata tattaggaga
     7561 tatttataat atgactgata aaatagttgt tgatactgga tttactccaa caaggtcatt
     7621 aacttctaaa actattttaa atataatctt aagagatgaa aaactaagaa aagctatatt
     7681 tggtgtaaat agtgataaac tacttacttt aaatgaatta aatacatttt tagtttctca
     7741 atctcttcct cctattttta cttatgatga aagatacaga gtacaaggca aggatggtaa
     7801 gtacacaaca aagagattct tagatgaaaa taagtttatt cttatgcctg acggcaagat
     7861 gggagatact ttctttgggc taacagcaga ggaattagaa cttagaaaaa acccagcaat
     7921 agatattagt tcagttggaa atataattgt agaacaatac tctactgctg acccagttgc
     7981 taagtggata aaagcagttg caacagcatt acctagtttt ccttatgctg accaagtgtt
     8041 tatgggtaca ataaattaga ggtgttgata tggaagttga aagactaaaa aaacttttag
     8101 gaatcagtaa ggaagattat tcaaaagaga tgatattaga gtttatatta gaagatgtgg
     8161 aggaaatagt taaaaattat tgtaatgtat ctgttattcc cgaagggcta aatagtactg
     8221 ttttaaggat ggctatagat atgtataaaa atgagaatct aggaagtgaa gatattgcac
     8281 taggttctat ttcttctata tcagaaggtg atacatcagt ttcatacaga agttcagcta
     8341 gtgaatttaa ggaatcgtta ctcaaagatt ataagtcaca attaaacaga tacagaaaaa
     8401 ttaggtggaa ataatgatgg ataagactag aaaagcaata gaaatgttat acagagataa
     8461 atgtactata attgagtatc agccagtcaa agaccctgtg acaaaacgaa ctaacaataa
     8521 agaagtgatt gtattagaaa atcaaccttg taagctttca tataaaaaca ttaattctac
     8581 agaagaagga aaagtagcta aacttgcaca aaccatcaaa ctctttatat ctccaaacat
     8641 aagcgttaaa gcaggttcaa agcttattat aactactcaa aataacataa caagagagta
     8701 tgtaagaagt ggagaatcag ctatatatcc aaatcatcaa gaaataatac ttgagttatt
     8761 taaggataaa gcataatggc tagatggggc agtgttgatt ttagagagtt taaaagagtt
     8821 tgtaaaaaga tggagaagct tacaaagatt gatttagata agttttgcaa ggatgcagca
     8881 agagaattag cagcacgatt acttgggaaa gtaattagaa gaacaccagt tgatacagga
     8941 ttcttacgac aaggatggaa tggagtggct tatgctaggt cgcttcctgt gtataaacaa
     9001 ggaaataatt atattataga agttgttaat ccgactgaat atgcaagtta tgtaaatttc
     9061 gggcatagaa ctaaagatgg taagggatgg gttaaaggac aacatttctt aacaatttca
     9121 gagatggaac tacaaagtca agttgataag attatagaga gaaagttatt aatcttgttg
     9181 aaaggagtat ttgatgctta ataatataat tgatggaata tctattaaat tagataaatc
     9241 atttggaaat gaatatacaa tttatagtga agatgtggag caaggtataa atgaaccttg
     9301 tttttttatt gttcctttaa atccaagcaa agtatcatat ccaagtggca ggacattaaa
     9361 aaagaactct tttgatgtac attattttcc tcgttcaaaa gataagagtt ttgaaataaa
     9421 tgaggtagct gagatgctac ttgaggaatt agagtatata gaaattgatg gagatttagt
     9481 tagaggcaca aatatgaatt ttgaaattat agataatgtt cttcatttct tcgttgatta
     9541 taactacttc actataaaaa gtaatgatac agaaaaaatg aatgatgttg aattatttgg
     9601 tggtttgaag agaggtgata attttgaata aaacattaag caaagaagat gactacaagt
     9661 ttactaagga gcaaatagtt aattctaaga agtatgtaaa tagaaaagac ttattaaatg
     9721 caattttaaa agaaaatgag ttatattcct tctcagaagt agaggaaata ataaataatt
     9781 ttatgaaagg agtgagttag atggctttag gtggaggaac atttgtaaca caaaataagg
     9841 tcctacctgg tgcatatata aattttgtaa gtgctacaag ggcaaccagt tcattatcgg
     9901 atagaggtat tgttgcaatg cctttagagt tagattgggg catagatgaa gaagtatttc
     9961 aagtaaccag tgatgatttt gagaagtatt caactaagta ttttggatat gattatacac
    10021 atgaaaaatt gaaaggttta agagatttat tcaaaaatat aaaactagga tatttttata
    10081 aattaaataa aggcattaaa gctagttgta gtattgctac agctaagtat agtggaataa
    10141 gaggtaatga tttaaaagtt atagttacaa caaatataga tgataatact aaatttgatg
    10201 ttgtaacttt gctagataat aagaaggtag atactcaaat agcaaaggtt attacagact
    10261 tgcaggacaa tgactatgta atttggaaga aggatgcaac attagaagca agtgcagggc
    10321 tggtatttac tggtggaact aatggagaag cagttacagg agcagaatat caaactttct
    10381 tggataaaat agaaagttat agctttaatg ctttaggatg tttggctaca acaacagaga
    10441 ttaaaagttt atttgtagaa tttacaaaga gaatgagaga taaggtagga gctaagtttc
    10501 aaacagtact atataagaaa agtgatgcag attatgaagg tgtagtgtct atagaaaata
    10561 agattaaaga taaagattta gttgaatcta gcttgattta ttgggtaact ggagctatag
    10621 ctggatgtga cataaataaa tctaatacta ataaaaagta tgatggtgag tttgatgttg
    10681 atgtaaatta cactcagata cagttagaag aagctttaaa aactggtaaa tttatatttc
    10741 ataaagttgg tgatgaagtt catgtgttag aggacataaa tacttttgta agttttacag
    10801 acgggaagaa tgatgatttt tcaagcaatc aatctgttag agtacttgac cagattgcta
    10861 atgatatagc gactttattt aatacaaagt acttgggtga agtaccaaat gataaatctg
    10921 gtcgtatctc gttttggaat gatgtagtta agcatcatga acaactgcaa aatatgagag
    10981 caatagaaga tttcaaagct gatgatgttt ctgtagaacc tggaagcgac aagaagactg
    11041 ttgtagtaag tgatgctgta aaagttatta gtgctatgag taagctttat atgactgttt
    11101 cagttagtta acaataagaa aggagaataa taatggcaca acaaataaaa gcaagagata
    11161 caataagtgc atctaaggca gagtgttttg taactataaa aggtaaaaga tataatttta
    11221 tgcaagctat taacttagaa gctaaaatgg aaaagaataa gagtgagata cctatattag
    11281 gtagtactac aaaaggaaat aaatcaacag gaagtaaata ttcaggaaat gcaacatttt
    11341 attataatac ctctatattt agagaattgt tgtatgagta taaagaaact ggtgaggata
    11401 tttacttcga tatacaaatt accaatgaag acccaacaag ttcagtgggt cgtcaaacta
    11461 taatactgga agattgcaat atggactcag gcataattgc taaatttgat gctgatgggg
    11521 agtatttaga tgaagatatg gattttactt ttgagaattg gaaattagtt gagaaattta
    11581 atatagcaaa tggtatggag taaaatacac atttatgatt tatgtatgtg tattttttat
    11641 ttataagaat aggagatgat taaaattaag gataaatatg agataaaaga ttcaatttct
    11701 tttgattata gcaataaaag acctttggaa gaacgtgtca gcgagatgta taaaaaggca
    11761 ggaaaatatc ttatagatat ttcagataag ttagcaacag atacaattga tggttcgtca
    11821 ttaaagccaa taatcataaa atttgaaata aatgaagctg gtgttgcaac aatagaaaaa
    11881 caaacaaaat atttggtgat ggaggtagaa taagaatatg ggagatttaa acgctttttt
    11941 aagtcaaaat gcaataaaag tagagaatag aaagtatgtg gcaagtgaaa ggtttatagg
    12001 agaagatgga aaagcaatcg aatgggaact taaagcaata gattcagata gggacagaca
    12061 attaagaaaa gattcaacta taagagtacc tatattaaat aaaaaaggga aagctacagg
    12121 acaatacact agtgaaacag attttaatac ttacactttg aaattatgtg tggaaactat
    12181 agtatttccg gatttacatg atgcagaatt acaaaatagt tatggtgtaa tgggtgcaga
    12241 ggagttatta acaacaatgt tgactcctgg cgaatataca gacctttcaa gtgaggtagg
    12301 agaggtaaat ggttttgata ggacttttga agataaagta gaagaagcaa aaaactaatt
    12361 gaaggaggcg attatgatgc tagtgtagct cattattgcc ttcataaatt caaatggaaa
    12421 ccacatgaat atacagattt accagacttc gagagggcat ttgttgctgc ttctatagat
    12481 attaaagtag aagaagaaat aaaagaagaa aaaaagattg ctaaagaagc tagaagaagt
    12541 agaagaagat aaaatatagg taaaatatgt aagaattata tgttataata attgtagcaa
    12601 gaagatgtaa tctacaattt atagagtgga gttcatactg ggataaaacc tacttcctaa
    12661 tgaaaggagg tgggaagtat ggacaatttt ttacttagta tattagctag cttgatagct
    12721 agtttaattg gatatatcgt ttgtagatgt atcaaaaacg taaaaagcca ctctactcgt
    12781 ggcaagagta aaagtggctg ggaacttggt tttaaaataa agttccgcaa atttaaataa
    12841 tttatatttt ttaaaattat gaacttcact ctaccgctaa atagattgta gttcttcttg
    12901 cctttattat accacaaatt agaaaaaata tgcaaagtac ttgttttaat agtaagtact
    12961 ttttttatgt gaaaaagaag gtgattgaat gaataaagat atagaactta cagaaaaaga
    13021 tttatattgt attgctagac atattcaagt gtatgtgttt aaaaaagatg atgaggtcat
    13081 aaaaaaggaa gataatccat gttgcagatg caagcataaa tttaagtatt atgagaatag
    13141 tgtatgtatc tatcattgtt cagtatttca gaagttatct aaaattacag gtcttaaaat
    13201 gggttttgga gtcaaactat aatgtaaaaa ttttgaatat atagtataat attcttataa
    13261 aatataattt agggggatat tatgagagaa ggaaagagaa gaagaggatg tctattttgg
    13321 tttatcatta ttattatatt ttctggggtt gttggagcaa tagcagggaa cagtactaat
    13381 aatggaagca ctgaaaaaca aaaagaagat ttaactaaat atattggtga agaaggtaat
    13441 ataggagatt taaaattaac tgttaatagt atttcaaaag ctagtgaaat accagtagct
    13501 tctggttatt tggcgtacac tccagatagt ggcaaatatg gtattataaa tttaacaata
    13561 aaaaatcaaa ctaaggaaag ccaatctttt atgctaaatt cattcacatt aataggacca
    13621 gataattcaa aatatgttcc atctttattg attgatgtag gaaataaata tataactatg
    13681 gatactgtaa atcctaattt agatgtaaca ggtaatattg catttgaaat tcctaaggat
    13741 ttactagttt ctgattgtaa actaaaatat agtggttcag agcaagaatt tatattaaaa
    13801 gaaaaataat atatagattt aaaatcaaaa atactaaaaa cacttactaa tgtaggtgtt
    13861 tttttaatga aaggatgtga tgataatgta aaaattttac taatatagta taatattctt
    13921 ataaaattat tatactgggg gggaaatgtt atgttttgtt caaattgtgg tgcagaaatc
    13981 acaggcatag gcaagttttg ttcaagttgt ggggttgctg tagaaactga aattattgaa
    14041 gataataata ttaaatcaaa tgacttaatc gttgatgcta atggaataga aataaatatg
    14101 actgaaattt atagaaaata tagaaaagaa aaagtaaacg caataaaaaa tgtaatggaa
    14161 ataagtggtt tgaatataaa ggaagcaaaa aaaatagtgg atttttcttt tgaagaatta
    14221 aaaattaatt ttattgatga tactatgagt aattcagaaa aggaaaaaat aatacaaaat
    14281 caaaatagaa aaaataatat tgaaaaagct caacaagaat cagttgcttg ttgccctaag
    14341 tgtggttcaa catccttgac agctcaaaaa aaaggttttg gtatagggaa agcaatggta
    14401 ggagctagtc taactggtgg tataggttta gtagctggaa atttaggagc aaagaaagtt
    14461 agagttacat gcttgaattg tgggaagcag ttttgggcag gcaaaaaata gatgtattaa
    14521 ttataaaaac acttactaat ttagtaagtg tttttattat ctaaattaac agaaaggaga
    14581 gtgaaaaaat ggctacaata cagacatcaa ttcgcatatt tgatggaatg acacctgcgt
    14641 ttcggcatat gacaactgct atgaacatag tactgagttc atttgagcaa ttacaaagaa
    14701 cttctagtaa tgcagtaaat gctaatagca taataagagc tagagaagaa ctagcaagag
    14761 cagaagctgg atttgataga ttagaacgac aaataagaga atcagataat caacagcgaa
    14821 aacttaatga ggatataaat aagggtgcaa gttctacaga tagattagtt ggaagtgcaa
    14881 agaagctagc agcaacttat ttaggtataa gaacattagg aggtctagga aatttaagcg
    14941 accagatgac aagtactaat gcgagacttg gcatgataaa tgatgggcaa caatcagatg
    15001 ctggacttaa taaaatgata tttcaatcag ctgaaaggtc aagagcatct tacttagata
    15061 ctgcacaaat agtgagtcgt ataggcatga acgcaggaaa ggcgtttagc agtacaaaag
    15121 aaattgtagg ttttgcagag caattaaaca aaaaatttgt aatagcaggc gcaagtactg
    15181 aggaaatgaa ctcggcattg ttacagctaa cccaagggtt gagttctggc gtattaagag
    15241 gtgaggaact gaatgctgtg tttgagtcag cacctaacat catccaatcg attgcagatt
    15301 atttggacgt ggacatagga aaaataagag gaatggcatc agagggaatg ttaacagcag
    15361 atattgtaaa aaactcatta cttgcagcag cagagcagac caatgcagag tttgaaaaaa
    15421 tgccttacac attttctcaa atttggactt caattaaaaa taatgcaatc atgatatttg
    15481 gtgttataca gaaaaaaatt gaacagtcta tgtctagtaa gggatttcga acctttatag
    15541 ataattttat aaactctttg tatgtacttg gaaatgttgc ttataacatt tttaatgaaa
    15601 ttataagtat attagggagc ccgttttttc aagcatttgt aaatgcgatt attgtaggtg
    15661 ttagtttaat agtgcaggca ctaggttgga taataacaca ggcattaaat attgctaatg
    15721 tgtttgctca gaactggagt attattgcac caatagtact tggagttgcg gctgctatgt
    15781 tagtgtataa caatgcgtta ttacttagta ttgcgaataa agttaaagat attgcactat
    15841 ctgccaaatc tttagtcatg agctttgcac atatagtagc agagtcttat agagcagcag
    15901 ctttagtagc aactacaata gcacaagacg gattaaatgc agcaatggca gcttgtccta
    15961 ttacttggat tttatatgga attatagcta tagttgttgc attttttgta gctatagcaa
    16021 tatttaatca cttcgcaggc actagtgtat ctgctatagg tgtagttgca ggtgcaatat
    16081 cagttgcagc ctctttcata ggaaacttat ttattgcaac aggaaattta attatagata
    16141 ttgtagcttt aatatacaat actttagcag gctttgcaga gttctttgct aattttttag
    16201 acgacccaat tggctctgtt ataagagcag tatccggaat ggctaatgct gtattaggca
    16261 ttataagaag tatcgcaagt gcatttgata ccgtgtttgg ttcaaacttg gcagatgcag
    16321 taagtggatg gcaagataaa ttgcaaggtt ggactgataa agtagcagga gaagctaaaa
    16381 taaaagttga aagaatggac cctaataaac tgcattttga cagatttaat tatggaaaag
    16441 catgggacgc aggatataaa tggggagata agttagaaac taatataaaa gataaatttg
    16501 atattagcaa aatggcagaa gatgcaaaga aaaaattagg attggatgat ttatgggata
    16561 aaaaatatgg attaggagat ggatttggtt cagcaggatt aaactctcct ctcaatgatg
    16621 cagcaaaagg agcaaaagac actgcgggaa atacagcaaa gatggctaaa acaatggata
    16681 aaagccaaga agacttaaaa tacttaagag acatagcaga acaggaaaca atcaatagat
    16741 ttacaggagt aaacataaaa attgatatga acaacacaaa caacataagt aaagataatg
    16801 acttagatgg aatagttaat gttctaactg aaaagttaaa tgatgcaatg gctgtttcag
    16861 ccgaaggaat agtttaggaa ggagagtgag aaaatggctt atgattttta cctagatgga
    16921 gtacaactac caatcgcacc accaaagctt gaagtcaaag tgacaaataa aaacaagaca
    16981 gttgatttaa taaatgttgg agaagtaaat atattaaaaa aagaaggatt atctgaaata
    17041 agttttgaag cagaatttac acataataaa ctaccatttt atcgtggaac ttttagggat
    17101 gttcaattct ttttaagtaa actggaacta ctaaaaactg attgtaagcc atttcaattt
    17161 attgtatcga gggaattagg taataaagta ctatttaaca ctaatataaa agtatctctt
    17221 gaagagtatg ctatttcaga agatgcagat aatggctcag atacaaaagt tgcaataaaa
    17281 ttaaaacaat atagagatta ctcaactaaa aagttagttc ctgcaactcc tgaaaagaca
    17341 aactatggta ggactccccc tccagtcatg aaaccaaaag aatttagacc agattcatcc
    17401 aataagccaa atggtaaaac atatacagta aaagcagggg attctctttg ggcaatttgt
    17461 caaaagcaat taggaaatgg ttcgttatac aagaaagtat atgagttaaa taaaacaatg
    17521 atggataaag ctaacaaggg aaaaaaatta agtaaataca ccatctataa agggcaggtg
    17581 ttgaggcttg tctgatgatt tagttctggc gaatgataga gatataaggc tagttattgc
    17641 acattgggaa gatttctacg aacctgtagt tttggatggt atcacatggg aaatagagag
    17701 acgaggaaca ccatctaaac ttgaatttac aatagtcatg gatgatatat tagagttttg
    17761 tgaaggtaac tctgtaagac tatattacaa aggtgtaggt atattctatg gatatatatt
    17821 tcagaaaaag agagataaag aaaatcatat caagatagtt gcttatgacc agctaagata
    17881 ttttaagaat aaagatactt atgtatatag taataaaaca gcaagtgaac ttgtaaagat
    17941 gttggctaag gattttaaat taaaatacaa tgtcatagaa gatacaaaat ataaaatatc
    18001 gagagttgaa gaaaataaaa cactctttga tatggtctta actgcactag atgatactct
    18061 aagagagaaa aaggaaatgt atgttttata tgatgatttt ggaagaataa cattaaagaa
    18121 tgttgcatca atgaaattag atactgttat gaataatgat gtaattgagg actttgacta
    18181 taattcaagt atagatagtg atacttatac aaaaatcaaa cttgtaagag acaatgagga
    18241 gtcaggaaaa agggatgtgt atattgctca agattcagct cacatgagaa gttggggaat
    18301 acttcaaatg tttgatacag tagataaaaa catgagtgaa gcagaaataa agcaaaagtg
    18361 tgatatactt ctaaaactat ataataagaa aactaagtca ttaagtttaa aaaatgtact
    18421 tggtgatatt agagtgagag caggttgttt agtacctgtt tttttaaatc taggagatat
    18481 tgaattgcaa aattatatgt tagttgagaa agtaaaacat acatttgaaa ataattcaca
    18541 tttcatggat ttgactcttg ttgatggaga tgaatttgct tcttattctt catcttcata
    18601 tagtagtgga aatactaata ataaagatga aaagaaaaat ggtcctgcac aaagtactac
    18661 aagtaaagaa gacaatgata tgataaataa attaaataaa gtatttaaaa ataagttatc
    18721 aaatacagga aatatatttg ttaaatattc taatgcttac aaagttaatg cagctttaat
    18781 ggctgctatt tctatacatg aaactggtaa tggaagttct tcactttgca aaaataaaaa
    18841 taatttcttt ggtatgaaag ggatgtcttt tagttctgta gatgaaggga taaaaaaagg
    18901 tattagcaat ttatcaagaa attatatcca tacaggaaga aaaacattag agcgtattag
    18961 agacaaatat gcacctcttt atgatagccc tcttaataaa gattgggtac caggagtggg
    19021 caaattttat aaacaaataa caggaagtac ctatacttct aataatgcag gtacaggagt
    19081 tggaagtaac gaagaagcag aaaagaattt aaaagataca atttatcaag ttcaaaacaa
    19141 taattctaat acatcaacaa acaataatag taaagtaagt aaagttattc aagaagcaaa
    19201 aaatcaactt ggtaagccat atgcttgggg aggcaatggt ccaaagagtt ttgactgttc
    19261 tggtttaatg gtatgggcat ttaaaagagg tgcaggaata aatctaccta gagtttcagc
    19321 agaccaatca aaagatagta gagggaaact attatgcaac ataaatgatg taaaagcagg
    19381 cgatttagta ttctttaaaa acgaacaagg aaaagtacat catgttggac tatatatagg
    19441 aaatgaccaa tatattcatg ctccacaaac tggtgatgta gtaaaaataa gtagtttaag
    19501 tggtagacaa aagaaaaagc gtgattttgc aagagctaga agattctttt aagtgaggtg
    19561 gtaatatgag tcaagaatta ttgcaaataa ttaaaaaagc tgcaatggat gcagtagaaa
    19621 caagtaatcc aattcaaatt gcatttggaa ctatagaaag tgttaatcct ttagtagtta
    19681 agatagagca aaaagcctct tttgaagaat cttttctaat acaaacagat acattcaaaa
    19741 aatatacaga taaaaaaata ggagataaat tagtcttaat tcgtatgcaa ggaggacagc
    19801 aata
//
LOCUS       DAELJO010000058        19468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELJO010000058 DAELJO010000000
VERSION     DAELJO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.058"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            55..360
                     /locus_tag="KPB88_001773"
                     /pseudo
     CDS             55..360
                     /locus_tag="KPB88_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            373..1278
                     /gene="truB"
                     /locus_tag="KPB88_001774"
     CDS             373..1278
                     /gene="truB"
                     /locus_tag="KPB88_001774"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG0054055.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1409..2338
                     /locus_tag="KPB88_001775"
     CDS             1409..2338
                     /locus_tag="KPB88_001775"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG0054056.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2442..2699
                     /gene="rpsO"
                     /locus_tag="KPB88_001776"
     CDS             2442..2699
                     /gene="rpsO"
                     /locus_tag="KPB88_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG0054057.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            2939..3784
                     /locus_tag="KPB88_001777"
     CDS             2939..3784
                     /locus_tag="KPB88_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0054058.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            3909..6020
                     /gene="pnp"
                     /locus_tag="KPB88_001778"
     CDS             3909..6020
                     /gene="pnp"
                     /locus_tag="KPB88_001778"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG0054059.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6286..7029
                     /locus_tag="KPB88_001779"
     CDS             6286..7029
                     /locus_tag="KPB88_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0054060.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7187..8434
                     /locus_tag="KPB88_001780"
     CDS             7187..8434
                     /locus_tag="KPB88_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0054061.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8469..8714
                     /locus_tag="KPB88_001781"
     CDS             8469..8714
                     /locus_tag="KPB88_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0054062.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            8724..9947
                     /gene="dapG"
                     /locus_tag="KPB88_001782"
     CDS             8724..9947
                     /gene="dapG"
                     /locus_tag="KPB88_001782"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0054063.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10060..10794
                     /locus_tag="KPB88_001783"
     CDS             10060..10794
                     /locus_tag="KPB88_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG0054064.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            10917..13328
                     /locus_tag="KPB88_001784"
     CDS             10917..13328
                     /locus_tag="KPB88_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG0054065.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13331..14389
                     /gene="mnmH"
                     /locus_tag="KPB88_001785"
     CDS             13331..14389
                     /gene="mnmH"
                     /locus_tag="KPB88_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG0054066.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14383..15717
                     /gene="rimO"
                     /locus_tag="KPB88_001786"
     CDS             14383..15717
                     /gene="rimO"
                     /locus_tag="KPB88_001786"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG0054067.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            15704..16246
                     /gene="pgsA"
                     /locus_tag="KPB88_001787"
     CDS             15704..16246
                     /gene="pgsA"
                     /locus_tag="KPB88_001787"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG0054068.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16534..17580
                     /gene="recA"
                     /locus_tag="KPB88_001788"
     CDS             16534..17580
                     /gene="recA"
                     /locus_tag="KPB88_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG0054069.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            17756..19297
                     /gene="rny"
                     /locus_tag="KPB88_001789"
     CDS             17756..19297
                     /gene="rny"
                     /locus_tag="KPB88_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG0054070.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         7952 a         1979 c         3783 g         5754 t
ORIGIN      
        1 tatgcaaaat tctgtgaagc aggaaccctg tgactttagt catgggaggt tcagtcaaag
       61 gtatctagta tagttttaac tcaagacatg atgaatagaa ataatattag ctttaatgat
      121 gttgatggaa taacaacata tacaagagac ataaaaggta tagaagttgg aattttattt
      181 aaagagaaaa aacaaaatga aattaaagtg agttttaggt caaaaaatta tgttgatgta
      241 agtgaaatag ccaagttatt tggcggtggt ggtcatgtaa gggctgctgg atgtactatt
      301 agagatagta ttgataatgc aaagaaaatg gtactagagg cagtgttaaa atcaatttaa
      361 ggtgacagta aaatgaataa gattataagt atattgaaac ccacaggtat gacatctcat
      421 gatgttgtaa gtagagtgag aaagatttta aatataaaaa aagttggtca tacaggtact
      481 cttgacccag atgcttcagg tgttttacca atctgtatag ggaaagctac aaaagtatgt
      541 gaagttattt taaacaaaga taaatcgtat atatgtgagc ttactttagg tataagtaca
      601 gatacttatg atgcttcagg tgaaatttta aaaaaagttg atgattttaa atttagtaat
      661 gaagacatag aaagagcttt tgacacacaa agaggagaga taaatcaact tcctccaatt
      721 tattcagctt taaaggtaaa tggaaagaga atgtgtgatt tagtaagaag tggtagacaa
      781 tctgaaatta ctttaaaaac aagaagagta aatataaaag acataaaaat actaagtata
      841 aaaggtaata aagtaatgtt ttatgttgag tgttctaaag ggacttatgt gagaagtatt
      901 tgccatgata taggagaata tctaggttgt ggagctcata tgtcgttttt gaatagaaca
      961 tcttctggta aatttgactt agataattca ataacattag aagaattaga attattttat
     1021 gaaaataaaa ctttagacaa atacttatat gatatagatt atgtccttga ttcgtttaat
     1081 tatgttgtat taaaccctaa tgctataaaa tattatagta atggtggctc aatagatgat
     1141 aaaaggtttt taaaaaataa tttcgataaa gatgatgaat ttgtaagagt atacagtaca
     1201 gataatttct taggattagg aaagttatca aaacacaata atacaataag tgttaaaagt
     1261 gataagatgt tcatataacc aatgtattta aaaaaacaac aaaagagata gtactataat
     1321 aacttctata gtctatctct tttacttatg ctataatagt ttgtgaataa gttaagagta
     1381 aattatattt tagcggggaa catttattat ggtgattata aaatctataa aagaaatagc
     1441 aaacataaaa gaaagtgtca ttaccatagg aaattttgat ggtgtacaca aaggacatca
     1501 agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa aatatacaga gtattgtatt
     1561 tacatttgca aatcatcctg taaattactt tagaccaaac tcaacaaaaa atattataag
     1621 caatgatgat aaaataaaat tattagaaga tttgggaata gatatagttg taatcatacc
     1681 atttgatgag tatatgacta aaatacctgc aaaggatttc gtagatgaga tactagtaaa
     1741 aaaattaaaa gctaaaaaga tagtaattgg acatgacttt acatttgcaa gggcaaaaga
     1801 aggcaatgtt aatctattaa aaagcttaga acatgaattt ggttttgagg tagaagtaat
     1861 aaaacccatc aaaatcaatg atgtaagagt gagtagtact tatattagaa gtttagtatc
     1921 ccagggtgat atggctaatg tcaaggaata tttgggtaga aattacaagt tagaaggttg
     1981 tgttatacat tctaaacacc ttggaagaac tataggattc ccaacggcaa atattgacct
     2041 taagaataat atgctcgttc caaagagagg tatttatgct tctattgtac atattggaga
     2101 tgagacttat tttggtgcta caaatgtagg ctataaccca acagttaatg gaaaaacttt
     2161 gtctattgaa acaaatattt tagagtttga tagagatatt tatggggaaa atataattgt
     2221 tgaatttttg gaaagaataa gagatgaaag aaagtttaat tcaatagatg acttaaaaaa
     2281 tcaattgtat aatgatacaa attatgtgta tgaaaactat gtttgcaaaa acaagtaatg
     2341 tatgataaaa tgattaagga aaataaccat tactcggcat ttgaatactc caatcaatgc
     2401 ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta aatgataaac aaatcagaaa
     2461 taataaaaga atatggaaga gctgaagggg atactggttc tccagaagta caaatagctt
     2521 tattaacagc tagaataaac gagttaaatg gacatttaaa aactcataag aaagaccacc
     2581 attcaagaag aggactatta aaaatggttg gtagaagaag aaacttatta gcttacttaa
     2641 aggaaaaaga tcttgaagga tatagagcat taatagctaa attaggatta agaaagtaat
     2701 ttcattcttt ataaaaaagc aggtatagtg tatacctgct tttttatagt taattcatta
     2761 taaagtaaaa tagttaataa taaaagtagt ttatatgtta taaaaaatta taaaatgaat
     2821 aatattgggt aagaatatta aaatagtata caaatacata atcaatttaa aaggaaagac
     2881 aggataaata tagaaatttt ataacaggat aattagttgg aataaggagg atattatttt
     2941 gaaaataaag ttagtatgcg atagtttgtg cgatattcca gatgaaatat cagaaaaaga
     3001 ttatatagag atagttccac ttacagttat ttttaatgat agggaatata tagaaggagt
     3061 agatataaag aaagaagagt tttataaaaa agtaaaagaa atcaagcaaa tacctaaaac
     3121 ttcacaagct acatatatgg agtttaaaga agtgtttgat aagttcataa cagaaggtta
     3181 tcatataatc tgtatgacag gggctgcaaa cgcatcagga acttttcaaa gtgctatgat
     3241 agctaaaaat gatgtaaatg aaaatgaaaa aatacatata tttgatacta gaaatttatc
     3301 tctaggtagt ggacaatatg ttataaaggc atgtgaactt cttgaagaag gactgggatt
     3361 tgaagaaatt atagatgaat tagaaaatac tcgcagtagt gtaagattgc tttttgcacc
     3421 atatacactt gattttttaa agcagagtgg aagagttcca gttgcaactg ctttgatagg
     3481 aaatatgtta aatataaagc ctatattttt ctttgataat ggggaagcta agttggtaaa
     3541 taaggttaga ggaataaaga atatagcaag taaattggtc gatataattt tagaaatgaa
     3601 tgaagggtct ttagaaggaa aaatcgtcac aataggttgt ggagataatt tacacgattg
     3661 tgaaatatta aaagatgaag taaataaaag aataaaagca agaagagtat tatttactcg
     3721 aggaggagtt tctatttgct ctcatacagg gccagatata ttggctataa gttgttctaa
     3781 ttagtagtaa tataaggttt ttgcatatat atgtgaagat aattttatat tgttaattta
     3841 tagtattttt gataatatag taaatagagt gatatacgtg ttttttaata agaggaggac
     3901 aagcattaat gtttgaacat aaaatattta aaatggattt tgcaggaaga gaactttctg
     3961 tagaaatagg aaagatttgt gaaatggcta gtggtagttg tatagttcga tacagtgata
     4021 gtatggtaat ggtaaataca actaaatcag ccaaacctag agacggaata gacttttttc
     4081 ctttaagtgt ggattatgaa gaaaaattat actctgtagg taagatacct ggaggttttt
     4141 taaagagaga aggcaagcct tcagagaaag ctatacttac atcaagattg atagatagac
     4201 caataagacc tttattccca aaaggattta gaaatgatgt tcaggtagtt gcaacagttc
     4261 tatcagtaga ccaagattgt acaccagata tagtagcaat gataggttct tcaatagccc
     4321 tttctatatc agatatacca tttaatggac caacaggttc agtatgtgtt ggccttgtag
     4381 atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga aaaaagttca atgcatctag
     4441 ttgtttcagg tacaaaggaa gcaataatga tggttgaagc cggagctgat gaagttccag
     4501 acgaggtaat gcttgatgct attttatttg ctcaccaaga aataaaaaaa atagttgagt
     4561 ttatagaagg tatagttgca gaagttggaa aagaaaaaat gcctgttgaa ttataccatg
     4621 caggagaaga aataactcaa ttggttcgcg aatttgcaac tgataagatg aaaaaagcag
     4681 ttcagacttt tgaaaagtta gagagaatgg aaaatatgga tagagttaaa gaagaaactt
     4741 tagctcactt tgaagaaact ttagaagatt ttgaagattt tgttggagat atagaagaag
     4801 tattacaaga tataataaaa gaagaagtaa gaaaacttat agtacatgaa aatgtaagac
     4861 cagataatag aaaacttgaa gaaataagac ctatatggtg tgaaactggc atgataccta
     4921 gagctcatgg ttcagccata tttactagag gacaaactca agtacttaat gttgctactt
     4981 taggtgcttt aggagatgtt caaaaactag atgggttaga tgaagaagaa aacaagagat
     5041 atatgcatca ttataatttc cctgcatata gtgttggaga agctagacca tcaagaggac
     5101 caggaagaag agaaatagga catggtgctt tagcagaaag agctttatta ccagtaatac
     5161 catctcaaga agaatttcct tatgctatca gattagtttc tgaagtatta agttcaaatg
     5221 gttctacatc tcaagcaagt gtatgtggct caacattatc tttacttgat gcaggtgtac
     5281 ctataaaaga tatggttgca ggtatagcaa tgggacttat aaagcatgat ggtaaggttg
     5341 cagtgctttc agatatacaa ggtatggaag accatttagg cgatatggac tttaaagtag
     5401 ctggaacaga gtatggtata acagcaatac aaatggatat aaaaattgat ggtatagata
     5461 aagaaattct acaacgtgct ttaaaacaag ctaaagaggg tagaatccat atactaggag
     5521 agatgagaaa aactatatct cagccaaaac cagaactttc accatatgca cctaagattg
     5581 ttaaaatgca aataaatcct gacaagataa aagatgtaat aggacctggt ggaaagataa
     5641 taactaagat aatagacgaa actggagtta agatagatat tgaacaaact ggggaagtat
     5701 ttatatcagg tattgagatt gatatgatta agaaagctca agaacttata aataatatag
     5761 tagttgaacc agaagtagga aaaacttata aaggtaaggt aagtagaata atgaactttg
     5821 gagcatttgt tgagatactt ccaggaaaag aaggattact tcatatatct catatagctc
     5881 atgaaagagt tgccaaagtt gaagatgtat tgaatatagg ggatgaagtt gaagttaaag
     5941 ttacagagat tgatgaaaaa ggaagagtta atttatcaag aaaagttctt ttaccaaaac
     6001 cagaacataa aaataaataa cttattatat aattaaaagc aaagtcttag gattttgctt
     6061 ttttaaatac aaaaaagtag ataattttaa aatgatagtt atggatggct tatagttctt
     6121 tttctgtcag gctttactct tataaagaag ctatttaaaa gctgattaat agagagcttt
     6181 attgatatat atagaatcac ttaagttata tttagtgcat aaacagagct tatttttaat
     6241 agtattaaat aagaaacaat ttgtacataa ttagggggag ttttaatgat aatgatggta
     6301 cttaataaga gtaagctcaa aaaaatcata atactaattt tagtagtgtt attagtatta
     6361 tcaggagggg taatggtatt aaagttattt aacaacaagc cagtatttaa tttatttaat
     6421 ggtgtagatt taccatatga aagaggtaca aaggataaaa gtggatatgt tgcattaacc
     6481 tgtaatatag accttggatg ggaaacagag tacgtagaat ccattttaga aacacttaaa
     6541 aaagaaaatg taaagattac atttaatgta actggaaaat gggcagaaaa gaataaggat
     6601 gaattattaa agataaaaaa acaaggacat gaaataggta atcatggata caaacattta
     6661 gattattcta cattatcata tgaagacaat tatgagcaaa tagagacctc taaaaagata
     6721 attgaagaaa ttataggtga aaaaactaaa ttttttcaag caccagcagg ttcttttggt
     6781 ccagaaacag taaaggctgc taaagcatta gggtatactt ctatcaaatg ggatgccgat
     6841 actatagatt ggaaatacaa agaccaacca gaggtaatca ttgatagaat gaagaaaaaa
     6901 gacattaaag atagtagtat aattttaatg catccaacca acgcaacgac aaaatgtata
     6961 gatgatatta tagctattgt aagagaaaaa ggactaaaac caggaaaact tagtgatgta
     7021 tttaagtaga caaagtataa atatttggtt aagattgtat aagttgtagt tgaattaatt
     7081 aaaagactga cttataatgt aatacatata aaaaataaat ataattaaaa catagtgttt
     7141 tcaataatcc aatatgaaac aaagaataaa ttgttaggag aatgtaatgt ataagacaaa
     7201 aatactggaa aatgggctta caataattgg tgaagaaata ccatatctaa agtcaataac
     7261 attaggtatt tggataaatg caggctctag gatagaagaa gcacaggtga gtggaacttc
     7321 tcattttata gagcatatga tgtttaaagg aaccaaaaac agaacatcaa aagagattgc
     7381 tagttcaata gataatttgg gtggtcaaat aaatgcattt acaagtaaag aatgtacatg
     7441 ttattatgta aaactaatag atgagcatat agatacagga attgatgtat tgagtgatat
     7501 gatactaaat tctaaatttg ataaaaatga tatagataag gaaagactta taatattgga
     7561 agaacttaag atgtatgaag actctccaga tgacttatct tatgatttat tagtggaaaa
     7621 tatatatgct aatgatggat taggaatgaa tataattggg acaaaagaat ctctatacaa
     7681 tataactaga gaatccatgt tagaatactt aaataagtat tatattccaa ataacgctgt
     7741 tatttctata gctggaaact ttaactttga tgatatggtg gagaaaatca aatcaaaatt
     7801 tggacactgg gaaaagaaaa atttaagtat agacataagt gaggctaagt ttaatccttg
     7861 ctttatatca aaaaataaag atactgaaca ggtgaactta gctatgtgcc taaaaggtat
     7921 tccttttgaa aatgatgaag aagtctattc aatggcagtg gtaaataata ttttcggtgg
     7981 aagtataagc tcaaggcttt ttcagaaaat aagggaagaa aaaggtttag tatattcaat
     8041 atactcatca cagacattat ataggaaatg tggtgaatta ggtatatttg caagcatgag
     8101 tacagaaaat ttacaagatg tatataattt aataaagaaa gaaattgaaa atataagaga
     8161 aaactatctt acagaaaaag aaatatctga aagcaaggaa cagttaaaag gaaattatat
     8221 attggattta gaaagtacta gcagtagaat gatgtctact ggtaaatcta tgttacttag
     8281 taaaaaagta aaaactacag atgaaatact tgaatgtata aataatgtta atattaattc
     8341 aataaagaag gttgttgata aggtattcaa tatcgaaaat attggtacat gtatagttgg
     8401 aagagatgta gaaaagattc tacacttgga ttaatattga aaaaagtaaa tctctggagg
     8461 tataaaatat gaatttatct gaaatagcag gtaaggaaat agttaacttg gtcacaggag
     8521 aaagattggg ggtcattgga gaatgtgacc ttatactaga tgaaactaca ggcggtatat
     8581 tagcgcttct aattccaaaa gaaaaaggat tttttgccag aagaaaagat aagtcatttt
     8641 tagaagtacc atggagaaat gtaaaaaaaa taggtaatga catgataatt atagaatatg
     8701 agggatatat ttaggaggaa aaaatgagta ttttagtgca aaagtttggt gggacatcag
     8761 tagaatcata tgaaaaaatg aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg
     8821 atgaagaatt acaattagta ttagtagttt cagcaatggg aagaaaagga gctccatatg
     8881 ctacagatac acttataaat ttgtgtagtg cagtaaatga tgagccaagt aaaagagaac
     8941 ttgatttaat aatgtcttgt ggagaaataa tatcaggaac aattcttgct aatttattga
     9001 atgcacaggg gatagattct gttttcttta ctggtcagca agcaggtata attacaagtg
     9061 atgaatattc taatgcaaag ataaagtata taaatcctaa aaaggtaaaa agagctttag
     9121 atgatggtaa ggttgtagtt atagctgggt tccagggtgt aacagatgat ggagaaataa
     9181 ctactcttgg aagaggcgga agcgatacat cagcagttgc tattggtaaa gctttagaat
     9241 gtgaaacagt tgaaatttac actgatgtgg atggtataat gactgcagac cctagagtag
     9301 aaccaaacgc taaagtattg agttttatag actatgagga agtattccaa atggctgaca
     9361 aaggtgcaaa agtaatacat ccaagagcag tacagttggc taaaagtgga aatataactt
     9421 tagccataaa gaatacaatg aatccaactt ttgaaggtac aaaaataggt tctttatgta
     9481 gacacttaga agacaatata gaatatgaac aagaaagaga ttttaaggtt gctgttgcaa
     9541 ataaagatag cgtagcacaa gttaagatta agtcagcaga agaagtgttt acagaagtat
     9601 taaatgagat agaaaagaaa cttataacta tagatatgat aaatttcttt atatcagaaa
     9661 aagcatttgt tgtagaagat gctgatataa aatcattaaa ggaaatacta gataaatttg
     9721 aattagatta tgaagtaaat agagactgtg ctaaggtaac attgatatgt tcaagaattg
     9781 ctgatgaaat gtctggtatc atgtcaaagg tagttagagg attatcaaaa gcaggggtat
     9841 ctcttttaca aacatcagac tcaaatatga ctatatcttg tttagttagt gaagaagata
     9901 tgcatacagc agtacatgct atacatcaac aattttattt aaaataaact gtataataca
     9961 attatttttt aatcaaattt aagaatttca tatatatctt aatatatttg atttatttgg
    10021 taaaactaaa ttaaatataa ttttgatagg agttttttta tgaaagatta tagagaagat
    10081 aatgaaagat ttgataaaga taattgtagt gaagaatcta gttgcgaaga gagagaatgt
    10141 ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc ctaaagatat tcaatgtata
    10201 actataattg gagaaattga gggacatttt ataggaaatc ctcaaaaaaa agctacaaag
    10261 tacgagcata taattccaat gctatactca attgaggaaa gcaatgatgt taaaggagtg
    10321 ttagttgttt taaacactgt tggaggagat attgaagcag gtttagctat tgcagaactt
    10381 ttaaacagta catctaaaaa agtagttact cttgttctag gaggaagtca tagtataggt
    10441 gttcctcttg ctacagcagg agattactca tttatagcac caacagcaac catgattata
    10501 catccagtgc gaactactgg tcttgtgatt ggtataaatg agacttttga gtattttaaa
    10561 aagatgcaag atagaataat tcaatttata ataagaacat caaatatcaa aaaagatgta
    10621 cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg atgttggtag tgtattaata
    10681 ggaaaagaag ctgtggatta tggtcttata gatgaagttg gaggactaaa agaagcactt
    10741 aaaaaattaa gagagcttat aaaagaatct gaggaagaaa aaaataatga ttaaaatttg
    10801 tttaaataat aaaatttatg atataatgat gtaataaagt aggagtgtta gtgttaaggc
    10861 attagcactt tttgctgtga aggggtaaaa cttgctaaaa ataaggagtg atattaatgg
    10921 ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag ttttaatgca gaatatcata
    10981 atttaataac aatatttata ggtgtatttt tattatatag tttaaattct aattctatgg
    11041 gatggatacc tgtgttaatg caaaatcttt ttaagggact atttggaggg ctttcaatag
    11101 caataccatt tatagtaatt ataacaggac ttttaggatt ttttgatgga aatgaatata
    11161 tatataggct aagaaagact aaactatact acataattat tttatttata tttgtatttt
    11221 atggattatt aaatgcagga actcttcctg tagatagccc actaaaaggt aacatgtttg
    11281 atgatgttat gaaattgggt gtaagtggac aaggtagtgg tttgatagca acaactattg
    11341 cgtattatat gagtaaaata tttggtatag caggtggatg gttaataagt atcttcgcac
    11401 ttatactttc tgtaatgttt atatttaata tttctataaa agatttggtg tcaaatgcaa
    11461 aatcaaaatc taaagcatct aaagacagca atttaacgtt taaagataaa attgcaaata
    11521 tgaaaaaatc tgcaatagat atgatgactg atgaagtaga tgatactact ataaataaaa
    11581 aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa taatgatgat gatgaagatg
    11641 aatattttga agaagaaaat agtgatggtg tagatgataa aactataaaa atagttggat
    11701 ttaataaggc agaagatgag tatttagaaa tattagaagg aactcaaagt atgccagagt
    11761 tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa tccagttgta gatactaaac
    11821 cagaaaaaaa agttgatata gctaaaccta atttaaatat agaaaaaact caacctatga
    11881 gtattgtagc agagccagtt aatgaggatt attcaaacta taaaaagcct agcattgagc
    11941 ttttaaataa agtaaataag aagtctgatg aaaatggtaa aaagaaagtg ttgaaaaatg
    12001 cttctctttt agaaaagaca ctatcagatt ttggagtgga agcaaaaatt aatcaagtta
    12061 cagtcggacc tactataaca agatatgaaa tacaaccaag tccaggagtc aaggtaagta
    12121 aaattgtaaa tttaactgat gatatagctt taagtttagc agctaaaagt ataagaattg
    12181 aagccccaat tcctggtaaa agtgcaattg gaatagaagt tccaaatgaa gaagctcaaa
    12241 tggttggtgt gagagaagtt ctagaaagtg aagaatttaa taattttaac tctcctcttg
    12301 caatgggact tggaaaagac gtagcaggaa aaattataat tggagatata ggtaagatgc
    12361 cacacttact aatagctggt tctacaggtt caggaaagag tgtttgtgta aatacattaa
    12421 taagtagtat tttatataag gcaaatcctg atgaagtaaa attactatta atagacccaa
    12481 aagtagttga acttgcaaat tataatggaa taccacatct attgatacca gtagttactg
    12541 accctaaaaa agctgcaaat gctttaaatt gggcagttac agagatgaat agaaggtaca
    12601 aattatttgc agatgctcaa gtaaaagatg taacaagtta taatgagaaa gcggaagaaa
    12661 aacttcctaa aatagtaata ataatagatg agttagcaga tttaatgatg gcaagtgcaa
    12721 atgatgtaga agattatatt tgtagattag ctcaaatggc aagagctgct ggaatgcact
    12781 tgattgtagc aactcaaaga ccatctgttg acgttataac aggggtaata aaggctaata
    12841 taccatctag aatagcattt gcagtatcat ctcaaactga ttcaagaaca atccttgata
    12901 tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt attctatcca cttggtgcag
    12961 caaaaccagt tagacttcaa ggggcattca tatcagagag tgagtcggaa aaagtaatcg
    13021 attttgtaaa aagtcaggtt aaagatggaa taaaatatga agaagatata attgaaacta
    13081 tatctaaggt taatacttca aagggtagtg atgaggatga gtttttgagt gaagcgatag
    13141 aatttgtcgt ggaaagtggt caagcatctg cctctatgct acagagaaga tttaaaatag
    13201 gattcaatag agcagctaga cttatagatt ctatggaaga aagaggaata attggagcaa
    13261 gtgagggaag taaaccgaga aaggtattaa taagcaaaca ggatttgcag aatttagaag
    13321 gtgaatagaa ttgtcagtta taaaaataga aaaagcactt aagctagata aagtaatatt
    13381 tgtagatgtg agaactgaag gagaatatga agaagaccat atattgaatg caatcaatat
    13441 gcctcttttt aaaaataatg aacataatga agttggaaca atttataaga tgcagggaaa
    13501 acatgaagca atacaaaaag ggtttgacta tgtatcgtat aaattaaagg atatatattt
    13561 acaagcagca gaacttgcat taaattatga taatattgtc atatattgtg caagaggcgg
    13621 aatgagaagt ggcagtatag taaatttatt atcatcatta ggtgtaaatg tatatcaact
    13681 tgaaggtgga tataaggctt atagaaattt tgttttagag tatttaaggc atgtaatgga
    13741 tacaaaaaac tttgtatctg ttcatggatt gactggtaca ggaaagactg atttgttaca
    13801 tttattagat gaaaaaggaa tagacgtact ggatttagaa ggcatagcta aaaacagtgg
    13861 ttctaccttt ggttttataa cttttgataa aaagccacca tctcaaaaga actttgaaac
    13921 aaaactattt gaaagtatat ttttttcgaa agagaattat atatttgtcg agagtgaaag
    13981 caaacgagtt ggaagtgtta cagttccaaa tgaaattttc gaggcaatga cacgagaagg
    14041 acatcatata ttattagaat gtgatattga aaatagagtt gatagacttt gtagagatta
    14101 tatttatgat aaagatgaag gtaatatatt gattttaaaa gaatgtataa acaagtttag
    14161 aaaaagattg ggtaataaag tagtagatga ttatattgat ttattagaat caggtgaata
    14221 taaagaactt gtaaaaagat atcttttaga atactacgac ccactttata tgcattcagt
    14281 agagaaatat aagtatagta agattataaa ttttaatgat acacaaaaag ctttagaaga
    14341 tgttatagac ttatatgaga gtatcttgga aggagaaagt gaatgttaaa aatagcattg
    14401 gaatcattag gatgctcaaa aaatttagta gatgcagaaa taatgatggg aattttaaat
    14461 aacaaaggct ataaattaat tggagatttt gaagaagctg atgtaattat agtaaataca
    14521 tgtgggttta tagagtcagc taaacaagaa tctatagata cgataataaa ctttgcagag
    14581 ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag ggtgtctagc ccaaagatat
    14641 tctgaagaat taaaaactga aattcctgaa atagatgcta tagtaggaac aggaagctat
    14701 caaaatatag ataaaatact taaagaatta agtgaaatgc atcaaattgt aagccttaat
    14761 gatatagagt ttgtatttaa tgaagattta ccaagatata tatctacacc tagctatatg
    14821 gcctatttaa aaattggaga aggatgttcc aataattgta cttattgtat aataccaaaa
    14881 ctaagaggta aatatagaag tagaaaattt gaagatataa ttaaagaagc taagaaatta
    14941 gctgaatctg gagtaaaaga acttgtggtt atagcacagg atacgacaaa gtacggtttt
    15001 gatttatatg gaaaagaaag attatcagaa cttttagaag aacttgcaaa aatagatgga
    15061 tttaagtgga taagggttat gtattcatat ccagaatcta taacagaaga attgatacaa
    15121 gttataaaga aatatgataa tatatgtagt tactttgata tgccaataca gcatgcaagt
    15181 aacaatattt taaaattaat gaatagaaaa actactaaag aagatatatt aaataaaatc
    15241 aatctgataa gaagtaatat accagatgct atacttagaa ctacaataat agtagggttt
    15301 cctggtgaaa cagaagatga cttcaaacaa cttgtggact ttgttgaaga ggttaaattt
    15361 gatagacttg gtgcatttgc atattcaaga gaagaagata caccagcaga tagattgcca
    15421 aaccatatag atgaagaagt aaaaatacaa agaagagata ctttaatgat gattcaacaa
    15481 aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa cttatgaggt gttaatagaa
    15541 gaacagatag aagataatgt atatacagga agaacacaag gtgatgcaga agaaattgac
    15601 agtatagtgt atgtaaaaag tgtagacaat cttgaagtag gagaatttgt aagtgttcaa
    15661 ataaatgatg caatggaata cgatttaatg ggagatgttt tatatgaact tgcctaacaa
    15721 gttaactttg tttagaatat ttttaatacc agtatttgtt ttaattatgt tgctcaatat
    15781 accaaataaa tttttaatag cttgtattat ttttataata gcttcaatta ctgatgcttt
    15841 agatggaaaa atagctagaa aatacaatct agtcactgac tttggaaaat ttatggaccc
    15901 attagcagat aagttattgg ttatttcagc acttacatgt atgatagaag accatcttgt
    15961 aagtagttgg atggtaatta taatagtggc tagagagctt acagttagca tattaagagc
    16021 tatagctgct gctgatggaa aagtaattgc tgctggaaac agtggaaaac ttaaaacgat
    16081 aactcaaatg gtatctatag tgtttttatt gttaggtgct cagtttgaaa atgtattgat
    16141 attaaatatt ggtgaaattc tcattcttat agctacacta cttacattat attctggatg
    16201 ggaatatcta tataaaaata aagagctttt tatgtcttct aagtaaaaaa ttatgcttta
    16261 ataaataaaa actaatgatt aataaataaa aaataacaac ttaaaatttt aaaaatgtaa
    16321 aaaagggtat actattatag tatacctctt ttcattttaa gaatattttc ttttgtgtat
    16381 ataattatta ataatggttt ataaataaaa tgtaatgaat aaaaatataa tcattagtag
    16441 cgtttttaaa ttgactaggt ttgatttatg ttatataatt aatatataac aagagaacaa
    16501 atgtttgtag ataaatgaag ggatggtata gagatgagtg tagatcaaga aaaattaaaa
    16561 gcgttgaatg aagctttagg taaaattgaa aaagattttg gtaaaggttc agtaatgaaa
    16621 ttgggagaag caacatctat gtctatagat gttatatcaa caggagcgat tggtttagac
    16681 atagctattg gtataggagg tctacctaga gggagaatag ttgaagtata tggtccagaa
    16741 tcttctggta agactactgt tgcgcttagt tgtgtagcat cagctcaaaa agatggagga
    16801 atagctgcat ttatagatgc agaacatgca cttgacccag tatatgcaaa agctttgggt
    16861 gtggatgttg ataacctaat aatatctcaa ccagatacag gtgaacaggc tttagagata
    16921 gcagaggcat tgataagaag tggagcgata gatataatag taatagactc agtagcagca
    16981 ttagttccaa aggctgaaat agatggagat atgggtgatt ctcatgtagg tttacaagct
    17041 agacttatgt cacaagcact tagaaagtta actggttcaa ttaaaaaatc aaattgtgtt
    17101 gctatattta taaaccagtt aagagagaaa gtaggaataa tgtttggaaa cccagaaact
    17161 actactggag gacgtgcact aaaattctat tcatcagtta gattggatgt tagaaaaata
    17221 gatacaataa aacaaggtga taaagttata ggtagtagaa ctagagttaa agttgttaaa
    17281 aacaaagtag caccaccatt taagcaagct gaatttgata taatgtatgg agaagggatt
    17341 tcaaaaattg gagacctttt agatatagct gctgatgtag atatagttaa aaaatcaggt
    17401 tcatggtata gttacaatga tactaaactt ggacaaggaa gagaaaatgt taaaaaattc
    17461 ttggaagata atttagattt aactactgaa atagatgaga aagttagagc attttacaat
    17521 ttaaatgaag aacatgaaga atcaggtact tcagtatcaa aagaaattgt agaagaataa
    17581 gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc acgctcccta ttagcttgac
    17641 acaatgttga aaaaattata aaattaagtg aggataaaat atctaaattt aattttaatt
    17701 attaggaata ttttttaatc atagtgatga cattttggga ttattaacga aggaggtgga
    17761 tgtcatagat agtgtagtaa tgattgtaat aggtgcagct gttggtatta tagctggata
    17821 ttttgttaga aaaaatatat ctgaagcaaa aattggacaa gctgaaaact tggccaaaga
    17881 aataatagat aaagcacacc acgactcaga aacagtacaa aaagaaaaat tactagaagc
    17941 taaagaagag atccataagt ggagaacaga agctgaaaga gaaaacagag aaagaagaac
    18001 agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa gaagtcttag atagaaaatt
    18061 acaaaattta gaatctaaag aagtaaacct aagtgaaaaa ttaaaggtag tggaaaagaa
    18121 agaagaagaa gttgaagtaa taaaaactca acagttagaa aaattagaaa gtatatcagg
    18181 aattacttct gataaggcca aagaaatcat cttaaccaat gcggagagag atgtaagacg
    18241 tgaaatgtct ataatgataa aagagataga aagtcaagct aaagaagaag ctgataagaa
    18301 atcaagagaa attataggat atgcaattca aaaatgtgca gcagatcacg tagccgaaac
    18361 tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa ggtagaataa taggtaggga
    18421 aggtagaaat ataaggactt tagaaacctt aacaggtata gaccttataa tagatgacac
    18481 tccagaagcc gtaattttat caggatttga tccaataaga agagaaattg caagaattgc
    18541 tctagaaaaa ttaatagcag atggaagaat acatccagct agaattgaag aaatggttga
    18601 aaaggcgaga aaagaagttg acaatattat aaaagaatat ggtgaacaag ctgcatttga
    18661 aactagtgta catgggattc atccggaatt aataagatta ttaggtagac ttaactatag
    18721 aactagttat ggacaaaatg tacttaaaca ttctatagaa gttgctcata tagctggtat
    18781 aatggctgct gaaataggag cagatattag attggctaag agggcaggtt tattacatga
    18841 tataggtaag gctgtagacc atgaaatgga aggtacacat gttgaaatag gtatggattt
    18901 acttagacgt tataaagagt ctaaagaagt tatacatgcc atgagtactc atcatggaga
    18961 ttacgagcct caaacagtag aggctgtatt agttacagca gcagatgcaa tatcagctgc
    19021 tagacctggt gcgagaagag aaactttaga agcatatata aagagacttg aaaaattaga
    19081 agaaatagct aattcatatg aaggtgtaga aaaatcattt gctatacaag ctggtagaga
    19141 aataagaata atggtaaaac cagaagctat aaatgatgaa gaaatacatc tattggctag
    19201 agatatgact aagaaaatag aggatgaact tgaatatcct ggacaaataa aagtaagcat
    19261 aataagagaa acaagagcta tagagtatgc gaagtaaaag aaataatatc taaataataa
    19321 gaaaatattt ttagaaattt aaatttgcca caagatatga taatatatct tgtggctttt
    19381 aaattgttta agtttaattt tatataatgc aaaaataatc taagtgctaa aagtgctact
    19441 tacaaaagat taataaaata aagcttta
//
LOCUS       DAELJO010000059        19212 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELJO010000059 DAELJO010000000
VERSION     DAELJO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.059"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(73..744)
                     /locus_tag="KPB88_001790"
                     /pseudo
     CDS             complement(73..744)
                     /locus_tag="KPB88_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
     gene            complement(771..1493)
                     /locus_tag="KPB88_001791"
     CDS             complement(771..1493)
                     /locus_tag="KPB88_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0054071.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPAGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEVFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(1493..1822)
                     /locus_tag="KPB88_001792"
     CDS             complement(1493..1822)
                     /locus_tag="KPB88_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG0054072.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRNGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            2152..3078
                     /gene="fba"
                     /locus_tag="KPB88_001793"
     CDS             2152..3078
                     /gene="fba"
                     /locus_tag="KPB88_001793"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG0054073.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3213..4844)
                     /locus_tag="KPB88_001794"
     CDS             complement(3213..4844)
                     /locus_tag="KPB88_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054074.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(5327..6364)
                     /locus_tag="KPB88_001795"
     CDS             complement(5327..6364)
                     /locus_tag="KPB88_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0054075.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(6383..7168)
                     /locus_tag="KPB88_001796"
     CDS             complement(6383..7168)
                     /locus_tag="KPB88_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0054076.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7201..8334)
                     /locus_tag="KPB88_001797"
     CDS             complement(7201..8334)
                     /locus_tag="KPB88_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0054077.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(8382..9509)
                     /gene="hadC"
                     /locus_tag="KPB88_001798"
     CDS             complement(8382..9509)
                     /gene="hadC"
                     /locus_tag="KPB88_001798"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG0054078.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(9509..10735)
                     /gene="hadB"
                     /locus_tag="KPB88_001799"
     CDS             complement(9509..10735)
                     /gene="hadB"
                     /locus_tag="KPB88_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG0054079.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(10750..11550)
                     /gene="hadI"
                     /locus_tag="KPB88_001800"
     CDS             complement(10750..11550)
                     /gene="hadI"
                     /locus_tag="KPB88_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG0054080.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(11589..12788)
                     /gene="hadA"
                     /locus_tag="KPB88_001801"
     CDS             complement(11589..12788)
                     /gene="hadA"
                     /locus_tag="KPB88_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG0054081.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            13465..14463
                     /locus_tag="KPB88_001802"
     CDS             13465..14463
                     /locus_tag="KPB88_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG0054082.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            14825..15352
                     /locus_tag="KPB88_001803"
     CDS             14825..15352
                     /locus_tag="KPB88_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG0054083.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            15379..15975
                     /locus_tag="KPB88_001804"
     CDS             15379..15975
                     /locus_tag="KPB88_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG0054084.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDAYDEIVFCGYGEPLMRIDELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16040..17623)
                     /gene="asnB"
                     /locus_tag="KPB88_001805"
     CDS             complement(16040..17623)
                     /gene="asnB"
                     /locus_tag="KPB88_001805"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0054085.1"
                     /translation="MINLEEKYSIRKDTCMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYKTEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVFIGRYWKPEFHPDYSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEEWFDESKLAQQTAEILGCKNSRYIITPEEYFASVPYVMYN
                     MEQPTGDASAIVFAIGCKAAVESTSICYSGEGADEFFGGYNIYHKAECYGENLKKFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIKVVKRIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(18201..19034)
                     /locus_tag="KPB88_001806"
     CDS             complement(18201..19034)
                     /locus_tag="KPB88_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0054086.1"
                     /translation="MNYIIKKVLNSSVVLVYDANDNEFILLGKGIGYGKKTGEYICDS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     K"
     gene            complement(19058..>19212)
                     /locus_tag="KPB88_001807"
     CDS             complement(19058..>19212)
                     /locus_tag="KPB88_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0054087.1"
                     /translation="VSESTKQMSKRYGYIYVDADDYGKGTYNRYKKDSFYWYKKVIEN
                     NSIDFK"
BASE COUNT         5957 a         3467 c         2337 g         7451 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtgcgt
       61 agctattttt taatcaaaat cttcttgaga agaagttact atattaacca taatctcatt
      121 agtatgaatc ccttttctaa ctacaagatg tctcaaatat cctttgtgat tcattgttct
      181 ataataagga agcttttttt cattgaaaaa ctctacacta gaaactaata tctttgagaa
      241 gtcttcatca accaacatac actcctcaac agtttgtata tctatatgct ttcctttttt
      301 atgaagacct aatgtcaaag gtccattttt tacttcatct ccaaaagtat attccatctt
      361 atttctataa tatttgtttt ctggacttcc ttctatccct aaaaactgaa atccaaataa
      421 atcttgcttt aaaaataaat ccataacttg tttttctttt atttctaatt gtttttcata
      481 tggcacagac aatagtgtac atcctccaca ctctctaaag tgcttgcaag tttcttcagt
      541 ttcaagtggt gagttctcta ataattctat catcttaact tcagctttct tacttctaac
      601 ttttttaact ccagctctca ccttttgacc acttattcca cccttcatat ggataactct
      661 atctccaact tggcttaaag atgtaccacc aaattccatt ttatctactt caaattctat
      721 aacatctttt ttcttcacag acattcctcc aatttactat agtatcttta ttatctttct
      781 tcaatcaatt ttacttcttc atctgttaac tctctatact cacctaactc taaactttca
      841 tccaatttta aattgcccat agacaatctt tttaagtata ctactttttt gcctacgctt
      901 tcaaacattc tttttacttg gtgaaactta ccttcatgta ttgtaagctc tatttctgat
      961 tcatcatcac tttttaatat atttaactgt gatggcatag ttttatatcc gtcatcaagc
     1021 actacacctt ctaaaaaaac ttctacatct tcttcggtaa ctactccatc aatttttgca
     1081 taataagtct taggcacatg cttcttagga gataaaactc tatgagaaag ctttccatca
     1141 ttagtcaaaa ctaataatcc ctctgtgtct ttatctaacc ttccaacagg aaatggttca
     1201 aaagctaagt atgataaatc aattaaatct aatactgttg ggtcatattt atctgtagtt
     1261 gctgatatat atccagcagg tttattcatc atcaaatata tatattctct atatataact
     1321 tcttctccat taaataatat ctcatcattt tcagtgtcta cttgagttcc tggatttgaa
     1381 acttcacttc catttactac tacagaccct tgtttacaat acttctttat ttcacttcta
     1441 cttccatagc ctaaatttga aagaatttta tctatacgtt gctttttagc cattaaaaat
     1501 gctctccatt ctcataatat ttttcttttt caaatactgt aaatgctaaa tccatacttt
     1561 ctacatctaa attatttctt atactatcag ttattattct tgctactata tcttgtactt
     1621 cttgccctct atcaaaccaa gctacttcaa caaaaggata tacatctgct attttaccat
     1681 ttcttattgc aactgacttt atacattcta tttcaaagta gtctcttgga cattttacag
     1741 cttcaactaa atcatttatc attttttcac ttattttaca tatatcattt tcattaattc
     1801 ctcttatttt tatctgtggc atagtttatc tcctttattt taaattttta gtgaattatc
     1861 atccacttaa ttcccttcga taacgtttga agtggtaact tcttggtcaa gacaactatt
     1921 gttatcagta tctccaaact aataaggtag ttcctaccct tatagttagc attatatagc
     1981 taattttcct aaattgttta tattctttat tattttattt aatcctattg tcttctattt
     2041 aacattatac ttgtaattta tagttattac aatttactat atactttttt tgatttaacg
     2101 ttataatata agtgatgggt aaattttaaa agttaatagg aggagtttaa aatggcatta
     2161 attactacaa aagaaatgtt taaaaaagct tacgaaggtg gatttgctat aggtgcattt
     2221 aacataagtg acttagaaca attacaaggt gttttaaaag ctgctaaagc taaaaattct
     2281 tatgttatga tacaagcttc tatgtcagct gttaaatatg ctggacctca tacattagtt
     2341 gaaatggtta aagctgcttc tgatgaaata ggtgtagacg tagctcttca cttagaccat
     2401 ggtccaaata tggatgctat taaaacttgt attgatgctg gtttctcttc agttatgata
     2461 gatggttctc attttgactt tgaagaaaat gttagaataa ctaaagaagc tgttgaatat
     2521 gcacactcta aaggtgttgt tgttgaagca gaacttggtg ttctagctgg aacagaagat
     2581 gatgttactt ctgatgttca caagtatact caaccagctg aagcagttga gttcgttgaa
     2641 agaacaggtg ttgattcatt agcaatagct attggaactt ctcatggtgc tttcaaattc
     2701 aaaggagaag ctaaattaag attcgatata ctagaagaaa tccaaagcaa attaccaggt
     2761 ttcccaatag ttcttcatgg tgcatctgct gttgaccaaa acgctgttgc tacatgtaat
     2821 gaatttggtg gaaatatagc tggtgctaaa ggtgtacctg tagatatgtt aagaaaggca
     2881 tcttctatgg ctgtttgtaa aataaacatg gatactgact taagactagc tatgactgct
     2941 gctatcagaa aattcttagc tgaaaatcca aaagaatttg acccaagaaa atacattggt
     3001 gctggtagag atgctataca agctgtagtt gaaagtaaaa tagatgatgt tttaggttct
     3061 gctaactcta taaactaata taacttttaa atatttataa aagaaaagag atgacttaaa
     3121 gtaattaaat ttacttctaa gccatctctt ttaagttgta tcaattatgt atcttataat
     3181 attttaatta tattgtcttt ttaaactgac agctaaacgt gaatatgtcc actctttatc
     3241 tttctatgga tagtagaagg atatatacct ataacttcag cagcctttgt tattgaacca
     3301 tacttattta tagccttgct tatcattatt tggtccacta ttttatatgc atcattaaga
     3361 ttcataattc catttaccac tattggtgat aaatcatctg tttcattatc aatcatatct
     3421 aaatttatca gcatgttaaa ttcatcttct ccaattacat ttttagctga taatactaca
     3481 aatctttcaa tgatgttttt taattctctt atgtttcctg gccaagaata ttcttctaat
     3541 aactcaataa ctttaggtga aacttctact tctctattgt acttttcatt gtaaagtttt
     3601 aagaaataat ttactaatgg tactatatcc tcttttcttt ctctaagagg aggtattttt
     3661 actggaataa cacttaatct atagtacaaa tcttgtctaa atttaaggtt atttctaagg
     3721 tcttcatgag aaatattagt agcacttata tatctaacat ctatttttat aggcttacta
     3781 ccacctattc tagtaatagt attttcttgt ataaccctaa gtaatttttt ttgcatctgc
     3841 attggtaact cacctatctc atctaagaaa atagttccac catttgcctc ttcaaaaagt
     3901 ccctttttac cttttttctt agctccagta aaagaacctt cttcatatcc aaaaaactct
     3961 gactctaata attcatttgg tatagctcca cagtttatag ctataaaagg tttgtctctc
     4021 cttgaactat acttatatat tgttcttgct attatttctt ttcctacacc tgattctcca
     4081 tttataaata tagaagaatc tgaggctgca gctctcttaa caactttttt tatattgtcc
     4141 ataatcttac tttgacttat atactctgtg tcttctgata tatcactata ttttatctga
     4201 gattcaagct taataactct tgaaatatct cttcctgtta gtactcctcc tgtaagtttt
     4261 cccttagaat cccatcttgg aactgctgta tatgcaataa gcttcccatc tgggtaaacg
     4321 atatttctat aaatcttttt cttcacttta agcatgttta atattattgg ttcttcaact
     4381 aatccttgtc tcacaaggtc tgttatattt ctaccaagta cctcagttct tttaaaaggt
     4441 aatatctcat cactgacagt gttcatcttt tcaattaccc catctttgtt aaacacaaca
     4501 acaacttcat ctatatgctc tattatatct tcaattactt tatcagatat tagagacttc
     4561 tcaaataaat aaactatatt attttcatta ttattatctt tcatattgta tatatgtgca
     4621 gtcatcctta actctctgaa agctattttc tttttactat ctgtcagtat ttctgttttg
     4681 tcaaaatttg aatctaattc atttatattt ctaggctttt ttatatctag aaaactaaaa
     4741 atactatcta ttagatgatt acagtatatt atttcgcctg attctttaca taaaatggct
     4801 ggattttcta catgttctat aagccctatt aaattattat ccattaatat tcgcaccttt
     4861 cattaagtaa tttattgaac tacatacaaa atttattgtt tgcttacaat aaataccatc
     4921 agcacatata atcaactcct tataatattc gtcttttcaa gtagtttatt tcattaaaac
     4981 gtaactaaga tataatttta atgattatag tttgttaaaa atttcatttt gcttttaatt
     5041 gtattttatc acatatttag taattatttg tctatttaat acaatttaat taaatttaag
     5101 atttatttaa aaaaacaaag ataatcctta cagaatataa ctcacaattt gtaagcaata
     5161 tacatataag gattatccag attatcttgt attataaaat tttattcaaa cagacttttt
     5221 aataactgac aaatacacac tagcaataac ttatcaaagg gttaataatt atgttactaa
     5281 ctaataagac ttattcaatc agtaatattt atatagtttg tatctattaa gcttttatag
     5341 cttctaataa atcatcaaca ctatctaatt tttctataag cattggaact atttcatgta
     5401 agtctccaac tactccatag tcagcgattt ctaaaattgg agctgctggg tttttgttta
     5461 tagcaattat gaaatctgat gattgcatac ctgctaaatg ttgtattgct cctgatatac
     5521 cacaagctat atataggttt ggtttaacag tagttcctgt ttgacctact tggtgagaat
     5581 ggtctatcca tccagcatca acagccgcac gagaagaacc aactactcca cctaatttgt
     5641 cagctaattt cttaagcatt tcaaatcctt ctggtccacc taatcctaga ccacctgata
     5701 ctataacatt tgcatctgta agagatacta aatctttctt agttttaact gtttctaaaa
     5761 cagtagttct tatatcatct tgagttattt tgtagtctaa tgctataact tcaccagttc
     5821 ttgtttcgtc tttttcagct ttatccataa caccaggtct aactgtagac atttgaggtc
     5881 tatggtttgg acaaatgatt gtagccatta tgtttccacc aaatgctgga cgagtttgtt
     5941 ttattttctt atcttctggg tctatttcta acttagtaca atcagctgtt aatccagttc
     6001 caactcttga agctattcta ggtgctaagt ctctacctat atgagttgct ccgaaaagca
     6061 ttatttctgg ttttatttca tctattgcat cttttattac ttttgtatat gcatcagttg
     6121 tatatttttc taaaagagca tcatctgcaa cataaacttt gtcagctcca aaagtgatta
     6181 attctttagc taatccatca acatcttttc ctaataatat tgcacaaagt tctgcatcta
     6241 cttctttagc taattttctt ccttctccta ataattctat aactactgga gctatttttc
     6301 cttctctttg ttctgcaaat atccatacgt ttttataaga acttaaatct tttatatcat
     6361 tcatagtttg tactcctcca acttatataa tgtgcttttg ttttagaccg attattattt
     6421 tactagctat ttcatcagct ttaccttcta agatttctcc ttttcctctt ggttcaggag
     6481 taaatgatct aaatacttta gttggtgatg cttttaaacc aacttcttct ttatttacat
     6541 ctaaatcatc aattgtccaa acttttacat catgtttata tgcttttact attttatcaa
     6601 cagacatgta tctaggtgta tttaactctt ttactgctgt taaaagtaca ggtgtactta
     6661 cttttattaa ttcgtatcca tcttctaatt gtctctgaac tattatatct tttccttcta
     6721 ttttgaagtc ttgtacatat gtaacttgag gaatgtctaa tttctctgca atttgtggtc
     6781 caacttgtgc agtatctcca tctattgctt gtcttcctgc aaatattatg tcataatcac
     6841 ctactttaga tattcctgct gcaatagtat ttgatgtagc ccaagtatca gctcctccaa
     6901 atgctctatc acttaataat attgcatcat cagcacccat agctaaacac tctcttagca
     6961 taaagtctgc ttgaggaggt cccattgtta taactgttat agttacatca tcatgctcat
     7021 ctttaatttt taaagcttcc tctaatgcat ttgcatcatc tgggtttaat atacttggaa
     7081 ctccatctct tataagagta cctgtttctt tatttattct tacttcgtta gtatctggaa
     7141 cttgttttac gcaaactaat attttcaaag tctatacctc caatttcatt agattattta
     7201 ttattttaat acgtttgatg aaattaccat tttttgaact tctgatgttc cttcgtatat
     7261 tgatacgatt ctagcatctc tgtacattct ttcgatttca taatctttta taaatccata
     7321 tccaccgtga agttgtaatg ctttataagc aacttcattt gcaatttctg atgcatagta
     7381 tttagccata gcagattctt gagcaactct cattcctgca tctctctttt gtgctgcatc
     7441 ataaactaat cctctagctg cacaaacttt tgtttccatg tcagctatag taaattgagt
     7501 attttggaat ttagctatag gcttaccaaa ttgaactctt tgttttgtat atttaacagc
     7561 ttcatctaaa gcaccttgag ctattcctag agctaaggca gcaacaccta ttctaccaac
     7621 ttctagagta cccattgcta tcttaaatcc ttgaccttct tttccaagta agttttcttt
     7681 tggaacttta acgttttcaa atattaagtc agaagtttca gttcctctta tacccatctt
     7741 atcttcatga gctcctgttg agaaaccatc ccatttactt tctactataa acgctgatgt
     7801 tcctctaagt cctttacttc tatctgtaac tgctattact atagctacat cacagaatgg
     7861 tccattagtt atgaaagttt ttctaccatt taatatatag tggtctccaa ctaattctgc
     7921 tgttgtttgt tgtccacctg catcagaacc agctcctggc tcagttaaac caaatgctcc
     7981 tattaattca cctgttgcta taccttttaa gtatttttgt ttttgctctt cagtaccatt
     8041 gtaaagtatt ggtaatgaag caagagaaga accagctgta acaaaagtag ctgttgaagc
     8101 acatcttcta gctatttctt ccattactat agcgtaagaa acatatcctg ctcccccacc
     8161 accatattct tctgggatat tactgcttat aaattttgca tcagctaatt ttttgattaa
     8221 ttccttaggc attacacctg ttttatccat ttctgctggt aaagtatcta attctttact
     8281 aacaaaatct cttactgctt ttcttaaaag ttcttgttct ttgttatata acatagcttc
     8341 ctccaatttt catattttat agtaaattta ttttaatgaa cttacgctaa actcatcatc
     8401 tcagcaaaag tttgaatacg agttctggct tgttcattat tctgagtttg ttggtcgatt
     8461 tcaacatata aagtaggtat tccactatct tctatatctt ttctaactaa aggataatcg
     8521 tattcttctg ggtcacagaa tttcatcata cagaaaataa caccatctat atctttcttt
     8581 ttaacttcat ctactataag tgacccacgt tttttcttag ggtcataagc taatgaacat
     8641 ccttctatgt ttgaccattg tcttgctaat ctttctaacg catcatcacc tgctggtaca
     8701 tctgttctga attgtcttgt ttcttgtgct aagtcgtcag ctacaactga tatattgttg
     8761 tcttctaaaa tgtctaatat atctttagaa tcagctaata tacctgttaa taaaactttc
     8821 tttccagaac agacttcttc tggcatagca tttaattttg ctattaaatc tttcactagc
     8881 tcagtatgtt cttcttttct cataaagaat ccactcttaa ttactaagct tcttattgat
     8941 ggttttatag tattagaatg tttataagct acttcaacaa agtctctcat agtttttcta
     9001 tgttcattgt aaacttctat actttcatga atttttgctt cttctatttc atatccacat
     9061 atttcttcaa gttctctttt tacgcctttg tactcactga ttaagtattt tacaccagct
     9121 tcaagttttc tattttgtgg gtgtactaat gatatatatt ttatatgagg tactgctgat
     9181 ttccagtttt gtcctaaaca aattagtgta tcacacatac ctggtataat aactccagat
     9241 aattcatcat aagcaccttt taatccatat tctaaacatg attgcattat tgaacatgca
     9301 aatgcaggga aatattgttt agctaaatct aattctgttt gtcctcccca tataccaact
     9361 ggaagcattc cagctgcatg tataatttct tctgggcaat aaactgggaa acaacctata
     9421 gcttttttgc cagtttcact tttatatttt tttacagccg catttggatt ttcaactact
     9481 tctttcattt tagataaaat agcttccatt atatctcacc tctattaagt ttttttcttt
     9541 cttccattac ttcaactaaa ccttgaattc ttgtttcaaa ttgagcatta gtgaaagctc
     9601 tagggtctgc ttggtcacca tcaaatccag catatggtaa tccagtttct tcagctgctc
     9661 ttctttgcat ttcatattgt attaaactca taagtttaca gcttctgttc atatgataga
     9721 atgctccatc acatttaccc tctactaaag aatcaactct atattttgtc atacggtcta
     9781 ggtttacatt gttaaacata gtactatatg ctactgccat tccatctaaa tcattaactt
     9841 catattgtaa tgcccaagca tgtgggtaaa cactacctgt catgttaact ccaaatttag
     9901 ctaatgtttt catcttgtat cctatatatg gccaacaagg tataccttcc atcattattc
     9961 tgtatttttc ttctcctctg aaagaagatt taccagtttt catgttgtcc tctaactctt
    10021 ctataagtaa cttaaatgct tctgtagttt cttttttacc tctagcacaa actattacag
    10081 ccatgtaagt aaataagtca aatccattca ttggagaagg agaagaatct gctggtaaac
    10141 tcatagaata cttccatagt cttccgtttt cagctgatat tttcattact tcttcaaact
    10201 tcttagggtc aaatttcttt cctgatataa tttctagttg ttttatagct tcttcaaatt
    10261 gagctttaat ataatctatt cttgattgag taacttcgtc ttcattattg aaagttgtat
    10321 caatcattat taaaggtata tctaattctc ttgaaatatt ttcataccat tttataactt
    10381 ggttacatat attgttacag caaagtagga aatctggagc tggcatatcc aaagcttcac
    10441 atccaccatt ttctaaaaga ccaaaatttg ttcttgcata tgcacatagg tcaatagaat
    10501 atcctttaga ttcagctatt tcacataatt ctaaagaacc ttttttagct gctactccag
    10561 ctgcttggtt ttctggatat aatacgttta agtcaaatac ttctgctaat tcttgaggaa
    10621 atactgatgt tgaccaacct acaggtcttc cttcttcttt agctttaaat gcatttgcat
    10681 attgttcagc taataaatca ttaattacta ctctagcttc ttttttttca gacattttca
    10741 tccctctttt tatatatttt tcacttcttt ttgtgattct tttgcttcat caaaagcata
    10801 taacgctgct cctaaagcac cagttaattg aggtatatca ggtacaataa tttctgtgtt
    10861 gatttctctt gccatagctc ttacaatacc actatttcta gcaactccac caaccataac
    10921 tacatttctt tgtactccta ttctttttac taggctagaa actctctttg ctactgaagt
    10981 atgaatacct gccactatat cttcaatttt tgcattttca gataaatgtg atataacctc
    11041 agactctgca aatactgtac atgtactgct tattgatact tcattttgag aattcataga
    11101 tatacttccg agttcagata catcaacctc tattattttt gccattacat ctaaaaatct
    11161 tcctgtacct gcagcacatt tgtcattcat aagaaagttt agtagtcttc cattattatc
    11221 taattttaat acctttgcat cttgaccacc tatgtcaata atggttcttg tctctggaat
    11281 tatgaaattt acccctctag catgacagct taattcactt atttgcttat cagcatctga
    11341 atagttcatt cttccatatc ctgtaactac aactttttta atatcttctc ttgcaagacc
    11401 tgtcttgcca tataattttt ctaaaactct tgatggtcca gtagtcccag taccagagga
    11461 tattgtttca gaagctacaa tatcttcccc attctttaag attactccct ttgatgcagt
    11521 tgaacctata tctaatccca ttgtgtacat ctttagcatt ctccttttaa aaaatctaga
    11581 attactaatt aatatcttac aactttacta tctttaaagt tatttatttt ctcttcatcg
    11641 tatcctaaag attttaatac ttctacagta tgttgaccta cttttggtgc tggtgtatat
    11701 tctttaattc cttcatttct aaacataact ggagtattaa ctaagacacc tgtatttccg
    11761 ctatcgtatg ttttcttaaa taagaagtca tttgcccaag cttgttcgtc atctaataaa
    11821 tcttcacagc tttgaatttt ttcaaatggt aagtctgctt cttctaataa agctgaccac
    11881 tcgtctaatg ttttttctaa catagcttct ccaactatct taactaaatc ttcaacatga
    11941 ttaaccattg aatctatgtt attatatcta tcatcttcta atatatattc tctatttata
    12001 accttacaga atttgcctaa ccacttgttg tattgtatta aagctaattg aatccatctt
    12061 ccatctttac atttatatgt agtcattaat gggctgtttg gattttctct tgataaaggc
    12121 atttcatttc cgtattgtgc tgttgttatc attgttccca ttccatatat agctgtatgg
    12181 aaaagactta ctgttactct ctcaccttta ccagtttgag cttttttatg taatgctgct
    12241 aaacttcctg ctgctagtgc tagacctgca tagtggtcac caaatcctgc tgctgtattt
    12301 gctggagatg ttcctttttc cataacagat tggctaactc ctcctcttgc gaagtatgca
    12361 gtatagtcaa atcctggttt atcttttaaa ggtccttttt caccatatcc taatatttga
    12421 gagaatatta atcctggata cttatctttt atttggtcat aagctatacc cattttttct
    12481 aatgcttgaa ctctaacatt agttacaaat atgtctgctt ctgataataa tttatgtaat
    12541 atttctactc cttcttttga ttttacatta atacttacac cctttttatt tccattttct
    12601 aattcaaaca tagggttttc atcatctgat gctggagatt taaatgttcc acccataact
    12661 cttattccat caccttctat aggttcaatc ttaataacct ctgcacccca gtcacctaac
    12721 atttttgcac aacatggtgc tgcgatgaaa cttgaaagtt ctactacttt aactccttct
    12781 aaaagcataa cgttttcctc cttaatagta tcaattataa aatttcgatt aaatcactca
    12841 taaaatactt ttttctgata ttaactgtct gatgtcaaag ttcctaatct tatctaaaat
    12901 aacttaaaaa ttattaaaat tattttttat tgttttattg tcttacacct atattgttaa
    12961 gcatttatcg tgccaacttt atatttaatc aaaatatcgc ttgatttgaa tgttttttaa
    13021 caatatgcaa ttgaataatc tttttcatat cgtggcatct tgccactatc cattgcattt
    13081 tgctattctt tctatgctta taaaagtttt ttttatatat ttttttatga aaaagcttga
    13141 catttcaaaa aaagttttca ttgctaaact caaaaacaca tgttatcatt gtaattaatc
    13201 agcaagttag ttaactaatt tcaaacttag ataatttttt aataatattt aatctaaaaa
    13261 ataacaattt ctttgataaa ttttagagta attattacat taaataaaaa attactaaat
    13321 atcaaaataa cacagggaaa acaatttcaa aactaaattt ttaaccttaa aaattttgta
    13381 taaaaaatat aaccttatat gctacatatt atttctaaat gaaaaattac cataataatt
    13441 taataattac aggagggtat tgatatgaaa atactagtat ttggagcacg cgattatgaa
    13501 gaaccagtaa taaaaaaatg gtctgaagaa cataaggatg ttcaagtgga tatttatcct
    13561 gaaaacatga ctgaagaaaa tgtagttaaa gctaaagggt atgatggtat atctatacaa
    13621 caaactaact atatagataa tccttatatt tatgaaactt taaaagatgc tggggttaaa
    13681 gttatagctt caagaactgc aggggttgac atgatacatt ttgatttagt taatgaaaat
    13741 ggacttatcg ttacaaacgt tccttcttat tcacctaatg caatagctga attagctgtt
    13801 actcaagcta tgaacctttt aagaaagact cctctagtaa agaaaaaagt ctgtgaaggt
    13861 gattaccgtt ggatagctga acttcttgga acagaagtta gatctattac agttggtgtt
    13921 ataggtacag gaaaaatagg tgctacttct gcaaaattat tcaaaggcct aggagctaat
    13981 gtaattgcat ttgaccaata tccaaatagt gatttaaacg atatattaac ttacaaagat
    14041 tctttagaag accttctaaa agaagctgac cttataacat tacatactcc tttacttgaa
    14101 ggaacaaaac atatgataaa taaagatact ctagctataa tgaaggatgg agcttacata
    14161 gtaaatactg gccgtggtgg tttaattaat acaggggatt taatagaagc actagagtca
    14221 ggaaaaatta gagctgctgc ccttgataca tttgaaactg aaggattgtt cttaaacaaa
    14281 aaaatgaatc ctggagaatt aactgaccca gaaataaata aacttctttc tatggaacaa
    14341 gttatattca ctcatcatct tggtttcttc actagtacag cgattgaaaa tatagtttat
    14401 tctagtttaa gtagtgctgt agaagttata aaaacaggaa ctgctactaa tagagtaaat
    14461 tagtatttta taaatatatt ttttcattaa ttagctatta tctaacaaaa tagcctttaa
    14521 tttcccaaat ataagagagt cagctcagta tatattgagt agactctctt atattatttt
    14581 ttatattaaa cttgtacacc tcttattcat agcaaattaa atttatatta acttatccta
    14641 actactccta ctttatatat tttacctatt tttacttttc taattatata catttgaatg
    14701 ggtacaatct ttgttttgca aaaaaaacac ctaacaaact atgaaaattg tatgattaat
    14761 attaatatat ttataagaaa acataacaat tatgttagct ctggattttt agggagggag
    14821 tatattggaa aaaatgaata aagttacttc taaaaaaact acttatattg ttacttcagc
    14881 attatttgca tccataatat gcctgacaat agcatatata ttacatattc cagttggtgg
    14941 aaacaatgga tatgttcata taggagatgc ttttatttat ctagctgcaa ctattttgcc
    15001 aacaaactat gctatagctg cttctgcaat tggtgctggt ctagctgatt tatctacagg
    15061 tgctgctatc tgggtaatac caactataat cataaaacca atacttgtat tatttttcac
    15121 ttctaaaagt gacaagataa taaataagag aaatatagtt gcttcggttg tagcaggtat
    15181 agtaggatta gttttataca tgtttgctga aggtataatt attggaagtt ttacaagtgc
    15241 atttgtaatg agcttacttg gcttattaca gccaatagga agttttattg tttttataat
    15301 cttaggtatg gcacttgata aacttgattt taaaaaacgt tattttaatt aagaatttaa
    15361 atttatggag ggaaaattat gaatatctta tatacaattg aaaatagtat ctatgtaaat
    15421 ataactaata catgtccatg tagttgtgtt ttttgtataa gaaacgaaaa agatgaagtt
    15481 gcaaatagtg gtagtttatg gttggagcat gaaccaagtg tagatgaagt aaaagaagcc
    15541 ttcaataaat acaacttaga cgcctatgat gaaatcgtat tttgtggata tggcgaacca
    15601 ttaatgagaa tagatgaact gatagaggta gcaaagttta taaaagaaaa aagtagcata
    15661 aagataagaa taaatacaaa tggtctaagt gatttaatac ataacaaaaa aacagctatt
    15721 ctgttaaaag atgttataga tgcagtatca ataagtctca atgccccaaa taaagaagct
    15781 tacaatagag ttactcaacc aaagtttgga gaaaaatcat ttgattatat gttagatttt
    15841 gcaagagatt gcaaaaaata tataaaagaa gtagcttttt cagtagtaga tgaaatatct
    15901 ccagaagaaa tagaagaatc taagcaactg gcaaaaaaac ttgatatacc attaagagtt
    15961 agacataaga attagcaagt aaaattataa aacattaaga atcacctgaa tagtctttca
    16021 ggtgattctt cctcttgtat taacatttta caaaatagat ttcataccac actaggaatg
    16081 tgtaaatcat ccaaatcttt ctccaattat cagagtttcc actaatgtag tcattaaaaa
    16141 tctcattaat ctcatctatg ttaaagaatt tatctgctat ctcactattg aatttatctc
    16201 ggatatcctt attatattgg tcatcagcca tccatatacg aattggcaca ataaatccca
    16261 atttatttct aaaagcaatc tcttctggta atgtttttga tgcagctgta cgaaaggcaa
    16321 ttttattctc ttttcctttc actttatatt ctgatggtat tctggatgca atatcaaata
    16381 ttcttgtatc tgtaagaggc attctgactt ccaatccaac tgcagtactc attttatcta
    16441 tattaaaata aatatctcct tccaaccata tttgaatatc tacatttgac atgttagtaa
    16501 gtgtatctag ttcttctgtt tcttcataaa ttctttttac cactttaata ggaagcacat
    16561 caggattgta tttcttaaga atctttcttt tctcaccctc attcataatg ttcgtatttc
    16621 caatataaaa tttcttcaaa ttctctccat agcactctgc cttatggtat atattatatc
    16681 caccaaagaa ctcatctgct ccttctccag agtaacagat actcgtgctc tcaactgctg
    16741 ccttgcatcc aatcgcaaaa acgatagcag atgcatctcc agttggttgc tccatattgt
    16801 acattacata aggtactgac gcaaaatact cttcaggtgt aattatgtac cttgaatttt
    16861 tacacccaag aatctcagct gtctgttgtg ctaattttga ttcatcaaac cactcttctt
    16921 cataaccaca tgaacctgtt ttttctatat ctgacattgc caatatataa gatgaatcaa
    16981 cacctcctga taggaataac tctgcagttt cattctctga ttttacctct ggcataattt
    17041 tctgaagtgt tgtatgaatt tcatctgtcc attcctcaat ggacttgctg tagtcaggat
    17101 gaaattcagg tttccaataa cgcccaataa atactgtttt attctgatag acaaggtaat
    17161 gtcctggcat aagcttcttt actccacgga agaatgtatt ttcacctgct acatatgtaa
    17221 aagacatata aatctgtaac atgtcttcat ttaactcttt cacaaatcct ggttgctcca
    17281 taatcttacg aatagatgaa ccatagagaa gcttcccatt ttcagtctta taataataga
    17341 aaggctttac tccaaactgg tctcttaggc agaaaagttt cttttccaca gtatcccata
    17401 atgcaaaggc aaacattcca tgcatgtgat ttgccatatc atatccccag gtcttgtatg
    17461 cttctactaa aattgctcgt tcacgctcat ctctacataa tgaagaatcg attccaagct
    17521 cctcacagag aactttccag tttctaatat gtccattgta ttctaggatt tttatcatac
    17581 atgtgtcttt tcgtattgaa tacttttctt ctaaatttat catatgtgta tcctttcata
    17641 ttaaaacatt taactcttaa atttattgat tgtttttatt tatatttatt tacttataat
    17701 cattgaaaat taactttttt caaagtaaac ccttttttaa ttagtactaa tatgctcttt
    17761 attaaggtca ccaatatgat tatcagtgtt ataacattga attttttatt cttcataaat
    17821 attgtcctcc tttatattat cagctaaata caatatatct tatattactt acatattctt
    17881 taagaaattc tgaagatttt ataaaatttt ctgtagtata attaattaaa aatacaatgc
    17941 tagttataaa gcatattttc tattaaacaa aaaatagtta aaataaattc ataaccacat
    18001 aaacttattt taactatttg catattttaa ttataaatat taacttaaat ttttaacagg
    18061 atacactcat aaaataaaca taatgaacaa caaaataata tgtctcagtt tctttatata
    18121 ctttaccttc tttcaatttt ctaccatgta gatgtataag ataatattag taattcactc
    18181 tattgctgta atttactcta ttacttaact cgtgcgatat ggacaaccaa gaaagctatt
    18241 tcctcatttg ttaacttttt tccatatttc ttttctagaa aatctctaac ttttaatgca
    18301 attaacatct cttttggatt tgaccttttc atttgattaa ataataaatc atcaccactg
    18361 cttaacatac tatctgaaaa aaatctttcc acaaaaaatt ttatatgtgt tataaatctc
    18421 atataatgca tactcttttt atctaaggtt ctattcaaag aaaaaacaaa aatattaatt
    18481 atttctccaa ttactttggc atacttaatc gtatcatatg aatttccatc tgaagccata
    18541 gcgtttgcaa aatggaaagc aatgttcgct gcttcttcct ctggtaattg aatacccaat
    18601 ctctcttcta ccatataaat tgcaaccatt ccaaccttaa actcatttgg ataataattt
    18661 tttatttccc aaaatactcg atttgtaatt tttattcctt tacgcatcct ttcaattgca
    18721 aagttaaaat ggtctgctaa tattaagtat aagtttttat taaaagatga gttcaataac
    18781 ttttctgcct ctactattat ttcctgtgtt atctttaaaa tgtcgacagg aatatcatcc
    18841 attaattcta aaaattgttt tgattttgta ttttcaacag ggacaaacat ttgattatca
    18901 ttggaatcac aaatatattc tcctgttttt ttaccatatc caattccttt ccctagcaaa
    18961 ataaattcat tatcatttgc atcataaact aaaacaacgc tcgagttcaa aactttcttt
    19021 attatgtagt tcattttatt tgctcacctt ctattggtca tttaaaatct atactattat
    19081 tttcaattac ctttttatac caataaaaac tatctttctt atatcgatta tatgtcccct
    19141 ttccataatc atcagcatcc acataaatat atccataacg ttttgacatt tgtttcgttg
    19201 attcacttac ca
//
LOCUS       DAELJO010000060        18983 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELJO010000060 DAELJO010000000
VERSION     DAELJO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.060"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            16..387
                     /locus_tag="KPB88_001808"
     CDS             16..387
                     /locus_tag="KPB88_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0054088.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNY"
     gene            519..1793
                     /locus_tag="KPB88_001809"
     CDS             519..1793
                     /locus_tag="KPB88_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0054089.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETSEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1904..2848
                     /locus_tag="KPB88_001810"
     CDS             1904..2848
                     /locus_tag="KPB88_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054090.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3064..4401
                     /gene="rsxC"
                     /locus_tag="KPB88_001811"
     CDS             3064..4401
                     /gene="rsxC"
                     /locus_tag="KPB88_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG0054091.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4424..5401
                     /locus_tag="KPB88_001812"
     CDS             4424..5401
                     /locus_tag="KPB88_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG0054092.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5401..5970
                     /locus_tag="KPB88_001813"
     CDS             5401..5970
                     /locus_tag="KPB88_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG0054093.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            5983..6576
                     /locus_tag="KPB88_001814"
     CDS             5983..6576
                     /locus_tag="KPB88_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG0054094.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6591..7166
                     /gene="rsxA"
                     /locus_tag="KPB88_001815"
     CDS             6591..7166
                     /gene="rsxA"
                     /locus_tag="KPB88_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG0054095.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7182..8153
                     /locus_tag="KPB88_001816"
     CDS             7182..8153
                     /locus_tag="KPB88_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG0054096.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDHNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8373..8957
                     /gene="maf"
                     /locus_tag="KPB88_001817"
     CDS             8373..8957
                     /gene="maf"
                     /locus_tag="KPB88_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG0054097.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTDESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9002..9661
                     /gene="radC"
                     /locus_tag="KPB88_001818"
     CDS             9002..9661
                     /gene="radC"
                     /locus_tag="KPB88_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG0054098.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9782..10792
                     /locus_tag="KPB88_001819"
     CDS             9782..10792
                     /locus_tag="KPB88_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0054099.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            10802..11695
                     /gene="mreC"
                     /locus_tag="KPB88_001820"
     CDS             10802..11695
                     /gene="mreC"
                     /locus_tag="KPB88_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG0054100.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11710..12198
                     /gene="mreD"
                     /locus_tag="KPB88_001821"
     CDS             11710..12198
                     /gene="mreD"
                     /locus_tag="KPB88_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG0054101.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAVIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12210..15188
                     /locus_tag="KPB88_001822"
     CDS             12210..15188
                     /locus_tag="KPB88_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG0054102.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15286..15969
                     /locus_tag="KPB88_001823"
     CDS             15286..15969
                     /locus_tag="KPB88_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG0054103.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            15993..16790
                     /gene="minD"
                     /locus_tag="KPB88_001824"
     CDS             15993..16790
                     /gene="minD"
                     /locus_tag="KPB88_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG0054104.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16808..17092
                     /gene="minE"
                     /locus_tag="KPB88_001825"
     CDS             16808..17092
                     /gene="minE"
                     /locus_tag="KPB88_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG0054105.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17177..18307
                     /gene="rodA"
                     /locus_tag="KPB88_001826"
     CDS             17177..18307
                     /gene="rodA"
                     /locus_tag="KPB88_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG0054106.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18348..18761
                     /locus_tag="KPB88_001827"
     CDS             18348..18761
                     /locus_tag="KPB88_001827"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG0054107.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT         7273 a         2175 c         3835 g         5700 t
ORIGIN      
        1 gagagtgatt tcaaaatgaa gtttaatttt tgtcataaca atttcaatgt tactaattta
       61 gaaaaaagtt tagattttta taaaaaagct ttagggctta aagaggtaaa gcgcaaggaa
      121 gcagaagatg gaagttttat attagtttat ttaggagatg gcattactag tcatacatta
      181 gaattaactt ggcttagaga ttgggataga ccatataatt taggagacaa tgaatttcat
      241 ctggctttag aagttgagga gtttgatgag gcaaaaaaac ttcataaaga tttagattgc
      301 atatgttttg aaaatgaaag tatgggaatt tattttatag cggacccaga taattattgg
      361 attgaaatac ttcctaaaaa ttactaattt taaggatatg tttataattt ctttacaatt
      421 aataagaaaa taagttatca ttaaattgta gggactaaaa atggataata atgttatgaa
      481 tttatgacta taaaaatttg ttggggggag tgatagtagt gaaaaaagct atagcagctt
      541 taggaattgg ggcagtagca gtatctgtta gctccataaa tgcaagtgct cttgaaaagg
      601 gaacagtaac agcaagtgcc ttgaatataa gaagtggacc aagttctgat tgcgataagg
      661 tagcaaaatt atacaaggga aaaactgtag agattttaga aaagtctaat ggatggtata
      721 aagtaagggt atcgagttct gttgttggat ggggaagtgc aaaatacata tcaacaagtg
      781 gttcgtctga aggtacatca agtcaaaata attcaacttc aagtgggaca actattagtg
      841 gaaatggtaa agtaaatgtg agttctagac tgaatgtaag aagtggtgca ggtactaact
      901 attcattagt tggtaaagca aataatggag atgtagtaaa gttactagaa caaagcaatg
      961 gatggtataa aataaaacta tcaaatggag ttactggatg ggctagtagc caatatattt
     1021 ctaaaacatc agaagatgtt ggaacgaaca attctagtaa ttcaaatagt actaataatt
     1081 ctgataaaaa accatctagc gaagaatcaa ttgaaggcaa gaatggtaag gtaacatctg
     1141 ctgtgagttt aaatgtaaga agtggtcctg ggacaagcta ttctataata ggaaaattaa
     1201 atggtggaga tgttgttgaa ttaaaagcta aaagtaatgg ttggtataag gtaaagttat
     1261 cgagtggaac aattggatgg gtgagtgcca gttatatttc tgagactagt gaaggtacaa
     1321 aagaaaaacc aaattcatca tctaaccaaa attctcaatc taatagtaac tcaaagccat
     1381 catttactgg aaattcagat aaatctacag caaaaggttc aacaatcgta gattttgcat
     1441 atactcttat tggaatacca tatcaatggg gagcttcagg gcctgataaa tttgactgtt
     1501 ctggatttac acagtatgtt tttaaacatt ctgtaggcgt ttctattcct agagtttcta
     1561 gagaacaggc taattttgga agtgcaattt ctatggggaa ttatgcacca ggagatttag
     1621 tttattttga cactgatggt gatggaacaa ctaatcatgt aggtatatat gtaggaaata
     1681 gtaaatttat ccattgtagt ggtactcaga ctaatcctaa taaggttaaa gtggataatt
     1741 taactagttc atattggtct aaagtattac ttggtgcaag aagatttgtt tagtaagtac
     1801 aatttcttat taaaaaatta gaagaaataa ttatttatat tcataaattg tgactcttaa
     1861 aaatatactt tattaataat ataattttgg gagggacaaa ctaatgaata gaagaaaaac
     1921 gatatacaga ggatttaata ataatagaca taagattgat ttacgtaaat atgttataac
     1981 gattgcttgc ttgtgtctaa ttggctatta ttcttataca aaaataaaag atagcaagat
     2041 attggagtat gtatcggcta aaataccatt tctaaacaat tcatcagata taacttataa
     2101 agatatatct gatgaattaa attcaataaa aaaagggaaa aaatctaaat cacaaaccaa
     2161 ttcagatgat aaacaagaaa ctaatccaga aaaagcggta aataacacta aagagccaga
     2221 agaagtcaag ttagctacta tagaaggctg ggatatgtat actattcaag ttgctgcaat
     2281 agataataat gatgatttga aaaaaataca gacatcatta gttaataatg acataccttt
     2341 ttcagttatg gagaaggatg gagtaaaaaa aatacaaacc tattcctctt ttgatgaaaa
     2401 tgatgttaga aagcagataa gcagtgtaag aaaggtgttt ccagatgcat ttttatcaca
     2461 cttagatgca cctatgttgt ctttagagta tacaagtaac tacgcttata tagagagtat
     2521 atctaaagaa ttaaataaat taataacaaa ctttaaagaa gaatcaagtt tttggtcgaa
     2581 tgctgaaaat aatgtagata tggaaaaata taatactatt ttgacaaata gaaaggcaat
     2641 atcacaaaat atatcaaaag aagctgaaaa gattgactat tctgaaatgc gtttatttaa
     2701 agataattta atagagtatg taaagaatgt gaatgaaaaa attgatacag cttcaaaagc
     2761 agcaaatgaa gaaaaatata gtgtaagtaa aagtctgctt ttatcatcca tgcaggagta
     2821 ctctatgttt ataaattcta taaaataaaa tacagaaaat ttggacttta gaaagctata
     2881 tgaaaaggat tgtttaatta agcaatcctt ttcctcggtt tgacttaata tagaggtatt
     2941 gatttataaa ataaattata aaaatacgga tattttttta acaattgtta agatttttta
     3001 atatatatag tatacttata gaaggcagaa aaaatgtttt tttatagata agggggtaaa
     3061 acaatgaaac tcttaacttt taagggaggg atacatcctc catatagaaa agagtactct
     3121 aacacaaagg cacttgaaaa agctcaagct ccaaagatag tttacattcc tcttcagcaa
     3181 catatagggg caccagctaa acctattgtt gaagttggtg atgaagtaaa gtttgggcaa
     3241 aaaataggtg aacaacaagg ttttgtttca tgtaacgttc attcttcagt atcaggtaaa
     3301 gttattgcta ttgaacaaca tgaagtacca ggaggttcag ctcaatgtgt agttatcgaa
     3361 aatgacttta aggaagaatt acatgagagt gttcaaccaa aaggtcagtt agaggatttg
     3421 agcaaagaag atatagtagg tatcataaaa gaggcaggaa tagtaggtat gggtggagct
     3481 acattcccta accatgtaaa agtatctcct ccaccagata gcaaggcaga agtagttata
     3541 ttaaatggtg cagaatgtga accatactta actgcagacc atagattaat ggttgaaaat
     3601 ccagaagatg ttgtgtttgg tttaagagca ttaatgaagg tattagatgt caaaaaagga
     3661 tttataggaa ttgaaacgaa taaaccagat gctatagaag caattcaaaa tgtagctaaa
     3721 gactatagcg aaatcgaagt tgttggcctt caagtgaaat atccacaagg agcagagaaa
     3781 caacttattt atgcgtgtac aggcaaagaa gttccatctg gtggattacc aatagcagca
     3841 ggtgcagttg ttgacaacgt tgccacagca gcacagatag ctaagtctat taaaacaggt
     3901 atgcctttgg ttgaaagaat cactacaata acaggtagtt gcatcaaaga gccaaagaac
     3961 ctaataacaa aagttggaac attagtttca gaaataatag aacaatgtgg tggatttaaa
     4021 gaagataaga agattggtaa ggttataatg ggtggaccta tgatgggaat agctcaatat
     4081 actactgaaa tagctactaa caaaggttct tcaggaattt tatgtttgga tgaggaagaa
     4141 tcacgtactc cagatattca aaactgttta agatgtggaa gatgtacaga tgtatgtcca
     4201 tcattcttac aaccactttt cataagtgca tattctttaa aagatgacta tgacacagct
     4261 gaatatcata gagcaatgga ttgtatagaa tgtgggtctt gctcatttat ttgtccagca
     4321 agaagaccat tacttcaatc tataagatca gcaaagagag aaataggagc aaagagaaga
     4381 aagcaagctg ctcagaaata aagaaagtta agggggaaaa acgatggaaa ataagttgat
     4441 agtatcatct tctcctcatg tgagaagtaa tgaagatact tcatatataa tgaaacaagt
     4501 tattatagca ctccttccag cagcagtagc aggagtatac ttctttagac ttaatgcttt
     4561 aagtgctatg tttttttgta tacttggtac agtaggtact gaatttttat atcaaaaatt
     4621 tacgaagcaa aaaagtacta taggagattt ttcagctgtt gtaacaggat tattattagc
     4681 atttaacgta ccagcatcac ttccttggtg gatgtgtcta gtaggaggaa tatttgcaat
     4741 attagtagtt aaaatggtat ttggtggaat tggatgtaac tttgtcaatc cagcacttgc
     4801 tgcaagagca tttttattag catcattccc agtagcaatg actgcttgga ctcaaccagg
     4861 tgttaactgg ataggtaaaa atttagatgc agttactaca gcaactccat taagcttttt
     4921 gaaaaatgga gcagcaggac tggcagatct ttctagcaat ggaattagtc ttgctgacat
     4981 gatgataggg aatattggtg gatgtatagg tgagacatca gcaatattat tattattagg
     5041 tggagtatac ctaatgtata aaggcataat aaattatgtt ataccagtat tttacatagc
     5101 aactgtattt atattaacat tccttttagg tggatttaat ataactttcg caatatatca
     5161 actgtttgct ggaggactta tgttaggtgg attcttcatg cttacggatt atacaacttc
     5221 gcctatgact aaaaaaggtc aaataatata cgctgtatta gctggtctta taacaactgt
     5281 tataagaatg tatggtggat atccagaagg tgtatcttac tcaatactat tagtgaactg
     5341 tcttgctcca cttatagata agtttgttag aaatagagta tttggggagg tggctaaata
     5401 atgaacagta tggtgagatt aggtggtact ttacttgcta taagtgcaat agctgcatta
     5461 gctcttggag ctactaatca ggttacagct ccagtaatag aacaaagaaa tatacaagca
     5521 aataatgaac ttagaaaagc agtccttcca gaagcaaaag aatttaaaga aatgaaggaa
     5581 gatacttaca aaggccttgg agatgatctt atagctgaag tgtatgaagg tttagatggt
     5641 tctgaagtgg ttggatatac tttaaaagct aaaccaagtg gatatggtgg agaaattgaa
     5701 gttatggtag gtatatcatc tgaaggtcaa gttacaggtg ttgacattgg taatatgtca
     5761 gaaacagcag gtcttggagc aaaagctaag gatgatgcat ttaagggtca atacaaagga
     5821 aaaactgctg agcctcttga agtagcaaaa ggaagtacaa cagcagataa tcaaatatta
     5881 gcaatatcag gtgcaactat tacatctaca gcagttacta caggagtaaa tgctgctata
     5941 gatgttttca atagtgctct taataaataa ggagggttaa ttatgaattt agctaaagta
     6001 tttaaaaatg ggctaataga tgagaaccca acatttgttc aagttatagg tatgtgtcca
     6061 acactagccg ttacaacttc agcaataaat ggaattggta tgggtctttc aacagcagca
     6121 gttttaatat gtgcaaattt agttatatct ttaataagaa aaattacacc agataagata
     6181 agaataccta tatttatagt agttatagct acatttgtta caatagtagg tatgctttta
     6241 aaagcatatg ttcctgctct tgataaggct cttggtatat atataccatt aatagttgtt
     6301 aactgtttaa tattagctcg tgcagaaagt tttgctttca aaacaggagc tatgccttca
     6361 atagttgatg gtgtaggtca aggtcttgga tttactgttg cacttacaat aataggtgca
     6421 gtaagagaat tacttggaaa tggtagttta tttggaatga cattatttgg agcatctttc
     6481 caaccagtat taatatttat attaccacca ggtgcattct taacattagg attcttattt
     6541 gcaggattta ataaattaag aagtaaaaaa gcatagtgga ggtgtaataa atgaatttaa
     6601 tacttttatt tttaagtatc gttcttgtta ataacgttat aacatctcaa ttcttgggta
     6661 tatgtccttt cttgggagtt tctaagaagg ttgatacagc agtagggatg ggagttgcag
     6721 ttacgttcgt tttaactcta gcttcaatta taacttattt tatacagatt ttattagtaa
     6781 aaactggaac tggattctta caaacaatag cttttatatt ggttatagct tctatagttc
     6841 agttcgttga aatggtaatt caaaagatga gtccatcttt atatcaagca ttaggagttt
     6901 atttacctct tataactaca aactgtgcag ttcttggtat agctctagtt aatgttcaaa
     6961 aaagttataa tttagtagaa acaataataa acggatttgg agccggagct ggatttactt
     7021 tagcaatagt aatattcgca ggtataagag aaagactaga attagctgat ataccagaag
     7081 catttaaagg tttccctata acgcttatat cagcaggttt aatgtcaata gctttcttag
     7141 gatttacagg acttataaag ctataaaaaa gaggggtgaa aatggtgata cttacagctg
     7201 tattagtttt aggaattatg ggcttaatct ttgggatagt actagatttt gcatctaaaa
     7261 agtttgctgt agaagtagac gagagagtgg aagctatact aggagtactt ccaggagcca
     7321 actgtggagg ttgtggattt ccaggttgtg gaggtcttgc aaatgcgata gttgaaggca
     7381 atgcaccagt taatggttgc cctgtaggtg gagcagatgt tggtgcaaaa gttggagaaa
     7441 taatgggaat aagtgctgaa gcaggtgaga aacaagtagc aaaagttatt tgtaaaggaa
     7501 cttgttcaag tgcaaaagat aaatatgagt atgaaggaat atcagattgt agagcagcaa
     7561 atgttttaaa ctcaggtgct aagatgtgta aatttggatg tttaggtctt ggaacatgta
     7621 aagatgcttg taaatttgat gctatttcta tagttgatgg aatagcagta atagatgaag
     7681 aaaagtgtgt aaattgtgga aaatgtaaag aagtttgtcc taagggtata ataatcacaa
     7741 aaccagaaag tcaagaagta gttgtagagt gtaatagcaa agagtttggt aaagctgtta
     7801 aggaaaaatg tactgctgga tgtattggct gtggaatgtg tgtaaaagct tgtaaatttg
     7861 atgcaataat atttgaagac aaaatagcta agatagacca taataagtgt gtaggttgta
     7921 tgcaatgtgt agcaaaatgc cctacaaaag taatatcagg tgacataact aagaaaaaga
     7981 aagttactat tgaccaagaa ctttgtgttg gatgtacagt gtgcaaaaaa caatgtaagt
     8041 ttgatgcaat agagggagaa ttaaaagaaa aacataaggt agatgcagat aaatgtgtag
     8101 gatgccattt atgtatggaa aaatgtccaa agaaagctat taaaattttg taatatggac
     8161 aaaataagga tatggaataa tttccatatc tctaatgagt acataaaaca gaggtatgga
     8221 ataatttcca tacctctatt ttatgtatat ttattctaat ggtaataaat ataacaatag
     8281 tgtaacaaaa aaggttcgac atttctgttg aattttttac aatatagtga tagactaaat
     8341 aggtacaaat tacgtacaaa tgaggtgaat aaatgaatat aatcttggca tcagcctctc
     8401 caagaaggaa agaaattcta gagaatacga atgtaagatt tgatataatt aaaaatgaaa
     8461 ttgatgagat tatattggaa ggtgaaacac caaaacattt agttatgaga ttagcttttg
     8521 aaaaaagtat gtctgtagca tctgaacata atgaagatgt tgttattgga gctgatacag
     8581 ttgttgtttt agacaatgct attttaggaa aaccaaaaga tgagtcttgt gcaagagata
     8641 tgttaaaaag actatctgga agagagcatc aagtcataac aggtattagc ttaataaact
     8701 tgtgcgaaga taaaaaggta attgactatg taatcagcaa tgtgaaattt aaaacgttat
     8761 cagagcagga tattgaggat tatctgaaaa ctgatgagtc atttgacaaa gcaggagcct
     8821 atggtataca gggttatgga gctttgttag ttgaagaaat tagaggagat tattttaata
     8881 tagttggtct tccaatttca aggttaggcg acctattaaa aaaatatttt agtataaatc
     8941 ttttttatgg agtgtgattt atctgaaaaa gtcatttaat tcaaccatta aggtaaaaga
     9001 aatggcacca gaggaaagac ccagagaaaa gatgcttgcc aaaggcgtaa aaagcttatc
     9061 aaatgctgag ttgttggcta tacttttaag aactggcaac aagaataaaa atgctattga
     9121 attagccaac tatataataa atagagatat tcagggtatt agacatttag aagatatgac
     9181 aatagaggag ctgtgtaata tagatggaat tgggttatca aaatcaaccc aaattaaagc
     9241 agctttagaa ctaggctcta gagtagctag ttttaaacct ataaagtata aaataaggaa
     9301 tccttgggat atacagaggt attatatgga tagcctaagg tatttaaaaa aagaggtttt
     9361 taaagcggtt cttttaaaca caaaaaatga gataatatct gatgtagatg tatctatagg
     9421 gactttaagc tcgtcattag tccatccaag agaggttttt aaagaagcta taagaagaag
     9481 tgcaagtaag ataatagtga tgcataatca cccttcagga agtgtagaac catcaagaga
     9541 agataaaaat atcacatcaa gacttattaa atgtggagaa ataattggaa tagaaataat
     9601 agaccatata attattggag atggattata ctttagcttt aaagaaaata tgataatttg
     9661 actttatatg tgataaaata ataggagaat gttttaaaca tgattcttaa tagtaggaag
     9721 gagtaaaatc atggctaagg aaaaaaagaa agaaaaaaaa ggttttttta gttttaataa
     9781 gatgacaaag gatatgggaa tagatttagg gactgcaaat acattagttt acataaaagg
     9841 acaaggtatt gttgttagag aaccatcagt tgtcgcaata agagatgata gtaaagaggt
     9901 attagcagta ggtgaagaag ccaaaaaaat gataggtaga acaccaggaa atatagtagc
     9961 tataagacct atgaaagatg gagtaatagc tgattttgac ataactcaat caatgattag
    10021 ctattttata caaaaagcgg ctgataaaaa aggtgttgta agtccaagaa tagctatatg
    10081 tgttcctttt ggagttacag aagtagaaaa gagagctata gaagaggctg caagacaagc
    10141 aggagctaaa gatgcattcc ttatagaaga gccaatggca gcggctatag gggctggatt
    10201 aaaagttgaa gaaccagaag gtaatatggt agttgatatt ggtggaggta cttcagaaat
    10261 agctgtaata tctttaggag gaatcgttac tgctaaatct ataagaatag gtggagacga
    10321 atttgacgag tctatagtgg cttatgtaaa aaaagaatat aatcttatga taggtgaaag
    10381 aactgctgaa aatgtaaaaa ttaacatagg ttctacattt aaagatgatg aagaaataaa
    10441 tatgcaaata agaggtagag atttaatctc aggattacct aaaacaatag aaatatgctc
    10501 tacagaagtt agagaagcat taaaagaacc tgtaagttca atagtagatg caataaaatc
    10561 tacactagaa agaactccac cagaattagc ctcagatata atggaaaacg gaataatgct
    10621 aacaggagga ggcgctttgc ttagaggtct tgataagctt attacacagg aaactggtat
    10681 gtctgtacaa atagcagaaa cacctcttga ttgtgttgca cttggaacag gtaaatctgt
    10741 tgaagaccaa gaaatctttg aaaaagtatt aatgatgaat actaagaatt agaagttggt
    10801 gatggccttg agatttgaca agaacaaaaa tgagaaaaaa aggattaatg ttaaagtgat
    10861 agcaacgggc gtagttgcta tcactttaat tggaattgtg ggtatatcaa ttggtaaatt
    10921 ttctagtggt agtccagtaa atttaggagt agcttcagat gctattacaa gtgtaggtaa
    10981 gggaataaat gatggatttt cttttatcaa aaatggtttt aaagatgtag caaattttaa
    11041 agataattct aaaaaggtaa aaaaattaga agaagaaaat gaaaaactta aaaaaaatat
    11101 gatagcttta aatgccaaac tagataaaac agaatcgcta gaagagttaa aaaagacact
    11161 gaactttgta caagaagagt ataaggcgac aagtatttca gcaagtgttg ttggtaaaaa
    11221 tgatggaaac tggtatgaga gttttgtcat aggtgcagga aaaaattcag gtgtaaaaaa
    11281 agaaagtata gttatgaatg gtagtggatt agttggcata gtttatgagg tttccaacaa
    11341 ttatagtaag gcaatttcac tattggattc taaggcatct gtaagcttta aactagcaaa
    11401 ggatgccaat gctaaaggta caattactca aaatactact ttagataata aagatagtta
    11461 taatagtaaa ggatatttgc aaggatatat gtttgactca tcatataatg taattcaagg
    11521 agatataata acaacatcag gtcttggatt cttcccagat ggtataccaa ttggcgaagt
    11581 tgaaaaagtg gttgatgata aagataagtc attaaaatat gtagttgtaa aaccttatgt
    11641 tgattttaag aatataaatg acgttgtagt catagaacca agaaatatag gataaagggg
    11701 ataaaaacaa tgaaaaaagt tttactttgt ctgttgggga ttgtgcttat gactatagaa
    11761 aatagtataa caaattatat agatattttt ggcataagct ttaatttagt tcttatatat
    11821 gtaaccataa tctctcttta tttagatgaa ttagaaggtg ggattatagc agtaataata
    11881 gggcttatta aagacataac cgttggtgga atttttggtg taaatgcatt aattttgttt
    11941 attattgcat atgcaattgg atacatgaga gatcagctat ataaaggtag ttatattatt
    12001 actttcgttt taatttttat aggcagttta attgattcaa ttgtcaatat aggtacatct
    12061 agtataattt atcaaagcta caacatatca acactttttg taaaaggtct actagtagca
    12121 ccaatagtga atagtttaat tggtttagta atatatcgct tatctaaaag agcagtttta
    12181 aaacttaaag aagattagtt ggtgatttca tggaagaaaa taaacaagtt gatagactaa
    12241 aaataattaa gatagtcatg ggcgtgatat ttttagctat tttagtaaaa ataatctata
    12301 tgactacatt taaatatgaa tattacaatg agttagctga gaataaaaca tataaaaagt
    12361 tggcaataga agctccaaga ggagaaataa aagatagata tggaagactg ttggcaggaa
    12421 ataaaaactt atttactgtt caagtttcag gtaacgatat taataagaaa gatgcaaata
    12481 agcattcaag agcaaatgaa atatcattaa aacttataaa tttgctggag agaaatggtg
    12541 aagaatatgt agatgagttt cctatatatg tagaaaatgg aaagtactat tatacatatg
    12601 acagagatat tagggaatat aaatcagaaa atggtattcc aaatgattat aatgctaagg
    12661 aaagttttta ttacttggta gacaaactta tatcagctgg aatattatct caagaagata
    12721 aaagattgga tgcgacaaga ttacaggcaa aactaaatga aaatggatat tatccaccta
    12781 ttttagttag taaatggatg ttcacagcag aaagagataa gagagattgg ttggctagtt
    12841 ataagataaa agaaactaaa ctgagtgcca aagaagcgtt tgaaaaagta agaaatagtg
    12901 atgcattaga aattgataaa agtttgagtg atgaagatgc aagaaaaata atggtcgtaa
    12961 gggatttgat aaaatcaaaa ggatattctc aatataatcc agtaactatt gctaaggatg
    13021 taggagaaac tacgattgct caaattgaag aaagtgctat ggaccttgtt ggtgtttcaa
    13081 tagcagtaga accagtaaga tattatccta atgggtcatt agcttctcat atgttaggat
    13141 atgtaggaaa aatgccatcc actcaaatag aatcatacct tcaaaaaggt tatgaaactg
    13201 gagatatggt aggtcttgca ggagtagaaa agagtaatga aagtagatta agaggaactg
    13261 atggttataa aatggttaaa gttgatgcat tgggtagaat cagtaaagaa attgaatcta
    13321 aaaaacctaa atcaggagat actgtgtatc ttacactaga taaagattta caagaagttt
    13381 cagataatgc attaaaacag ataatcgaag ttgcttctaa aggaggtact tttaaaagta
    13441 aatttgggga caaaccaatt agtgcatatg caggaaaagc acagtcagca gctcttatag
    13501 ctattgatgt taaaaatgga gaagtattag cttcatcaag ttatcctaat tatgacccaa
    13561 ataaatttgc aaaaggtatt tctacagagg attataaagc acttcaacca aaaaatccaa
    13621 atgacttact agcaggaagt ccacttttaa acttggttac acaaggggaa tttcagccag
    13681 gttcttcatt taagatggta acttcaatgg cagcgttaga aaatggtctg gatccaaact
    13741 ttactataaa tgaccctggg gttataatgt taggtaaaaa atcttttggt gactatgtat
    13801 ggaaccatgg tagaggaaat catggtatga caaacttata taaagcaata caagagtctt
    13861 gtaatatata tatggcaaca attggtacag gtaaaacatg gccagatgga aaaagcattg
    13921 gtatagatat gaatgcaaat aaaatacttg agtatgcaaa attatttggg cttgaccaaa
    13981 atacagggct tcaagatgaa gtagaagaaa gagcaggtaa agttcctagc acagaagata
    14041 aattgaaatc aacacaagct cttctaaagt caaatttaga aagagaaatg gcaaatgatt
    14101 ttgttgatat aactagagaa aaaaatccta aagaatatga gaaaagaatc aatgagattg
    14161 taagctgggc agcagaaaag aaaactcctg gtagagttga aacaatgaat aggctaaaaa
    14221 aattggatgt taaagaagat agaattgaag aagtagcaga tttagcagta ttcagttatt
    14281 ttaactttgc aaaatggagt actgcagata cattcaactt agctatagga caaggtgaga
    14341 atgcctatac accagctcaa atatcaagat atgtagctgc tatagcaaat ggaggtaatt
    14401 tagtagaatt atctgtagtt gatagagctg tttcaagtga ttattcatca gttaaaatta
    14461 atgaccagaa gaaagtcgaa aaaatacctt ttaaaaatcc agataactta aaagaattaa
    14521 caaaaggtat gaaattggtt gcaagacaag gtacagcgaa gagtgctttt gctgattttc
    14581 caatagatgt agcagcaaaa actggtacag ctgagaaaag tgggaaaata cctacagata
    14641 acgagtatga atatttaaaa agtcatatgt catcttataa tgtcaaccta aatgatgcga
    14701 ttaaactagc tgataagatg aaagcagaaa aagaaaaaga gctttcatta gctaaggaaa
    14761 aggaaataaa aaagaaatta gaaaataaag atttgaaaga cgaagaaaga aagaaattag
    14821 aggaagaatt agaagatgga gtaaaagtta gattagagga cactgataag gtgaattctt
    14881 catatctaag aaaagctata aaagaattaa atccaaaaat aacagatgac caaatagata
    14941 gatttaaaca agattatggt tcttttacgt ggactgttgc ttttgctcca gcagatgacc
    15001 cagaaatagc tgttgtatgt gtaattcctc aaggtgattc aagtgtattc tcacttttac
    15061 caactagaga agttattgga acttatatgg gattagagcc aactaatagt aaaaatgaca
    15121 acaagacgaa tgatgttaac aattcttctg acgaaaatat aaatttcgag tcacaaatta
    15181 atagatagct caatctatgt ataaacttta gctttatata gaaaaaataa aagggtttaa
    15241 agcgtatttg aagaatataa atgatatatc actctggagg ttgttatgtc tttaagagaa
    15301 atttgtagtc aagaactagt agagttcaag ggtaataaaa gagggataat agtaaatata
    15361 aaaagagaag ctccatttga ggaaatacag gaaaaaataa taaacaaatt ggaagcatat
    15421 gttggctttt ttaatggggc taaaattagt aaaattaata gtgattgttt aactgacatg
    15481 gagattctag aacttaaaga aggaattact tctagatttg atgtggaatt tgttgaagat
    15541 caaaaaattg aagaaaatag taattttcca acaaaatatg tcaataccct aagatctggt
    15601 gaaaatattg aatttgaagg tgatgtagtt atattgaatg atatgaaacc tggttcaaaa
    15661 gtcctttcaa aatcaaatac agttgttatg ggagatataa atgctggtgc taaggttgta
    15721 gcaggaggta atgtttttgt aatgggtaaa atagaaggtt ttgtacatgc aggtgcagaa
    15781 ggcaatgaat ttgcatatgt tgttgctgga aatcttaatc ctaaaatatt acaaatagca
    15841 gataatattg cagaagctcc agatgatgaa gaaaattatg aaagtgaatc agaaattagc
    15901 ccagaaatag cctttgtaag taatggaaga attgtaatcg aaagctattt gtcaaagtta
    15961 gacaaataga aagaaaaata gggggtagta ttatgagcga agttatagtt ataacatctg
    16021 gtaaaggtgg ggttggaaaa actactaccg cagcaaactt aggaactgcg ctaagcctag
    16081 aaaataaaaa aacagtagtt gtggatgcag atattggact tagaaactta gatgtagtaa
    16141 tgggtcttga aaatcgaata gtttatgaca tagtagatgt agttgaagga acttgtaggc
    16201 ttaagcaagc tctaataaaa gacaaaagat ttgataatct atatttatta ccagcagcac
    16261 aaactagaga taaaaatgct gtctcagttg agcagatgat tgacctgtgt gagaaactaa
    16321 aagaatcttt cgagtatata ataatagatt gtccagcagg tattgagcaa ggctttaaaa
    16381 atgcagtagc tggggcagat agagctattg tagttactaa tccagaaata tcagcagtaa
    16441 gagatgctga tagaataata ggtctattag aagcaaatga aataaaagag ataagattag
    16501 ttataaatag aattagaaat gatatggtta agcgtggaga catgatggat aaacaagata
    16561 taatagaaat attagcaata gatttgttag gtcttgttcc tgatgatgaa agcataatta
    16621 tatcaacaaa taaaggagag ccagctatac ttgattctaa gtcacttgct ggtcaagcat
    16681 acaaaaatat cgcaaaaagg atactaaatg aagaagttcc tctacttgat cttgaagttg
    16741 aagatggatt ctttggtaga cttaaaaaaa tgtttagcat ggctaagtag caaaggagga
    16801 tacatctgtg ttagatttat ttagagtttt ttctaacgag gctaaaacta gtaaatctgt
    16861 tgctaaagag aggttaaagc tagttttagt tcatgataga gtagattgtt caccacaact
    16921 tttagagatg ataaaaacag acattttaaa agtaatagct aattatgcag aaatagaaga
    16981 tgatggtctt gaaataaaaa tgtctaaatg cagaggtgag catgatgaca aacctgtatc
    17041 tgcattagtt gctaatatac cactaaagaa tataaaagat agatgtatgt aattagtgct
    17101 taaaaggggg atatccttat tggtatatgc cccttttatt taattatatg tttaaagata
    17161 gaaagacggt gattctttga aaaaaatcaa tattaattat aagtcgacaa ttaagttgat
    17221 aaaacagtta gactggaaac ttatagttac agtattagct atctttattt ttgggcttgt
    17281 tatacttagt agtgcaacac atgctaattc aacaggttca tataatcaat tgattaaaca
    17341 aggattagct tttgtacttg ggataggaat gattatagtc atattatttt ttgactataa
    17401 ccttctaggc aggtattata aagcgcttta tataataagt cttattttgt tagccatagt
    17461 tttattacct ggaattggga cagtaaaagg tggagctagg tcgtggatta acttaggacc
    17521 acttgacttg cagacatctg aaatagtcaa attaacattt gtactaagtt atgcaaagat
    17581 acttgaatcc aaaaaggata aattaaatac gttaaaggaa gttatgcctg ttgtagttta
    17641 ttctcttcca tttatcggat tattgattgc acaaccagac ttgggaacag gaattgtatt
    17701 ttgttgtatg atatttgcaa tgctgttcac agcaggtctt agctcaaagt tgataaaaag
    17761 aggaataatc atactattag tgtcaatgcc actgatgtat ttaatgatgg cagaccatca
    17821 aaaggtaaga atagaagctt ttctaaatcc agaagatgta actctaaaag gaaactatca
    17881 agttatgcaa tctctgattg caattggttc tggaggcgta actggtaaag gactttacaa
    17941 tggaagtcaa aatcaagaag attttttacc tgtacaagat agtgatttta tatttgctgt
    18001 tgtaggtgaa gaacttggtg taataggaat ggcagtgttg ataatcttat ttatgatatt
    18061 cctattaagg cttttagcaa ttgcaagaga tgcaaaagat ttttatggaa ccctcatagt
    18121 ggttggtgtt atgggaatgt ttggttacca gataatccaa aatataggta tgacggttgc
    18181 gttaatacca gtaactggag taacattacc ttttgtaagt tatggaggaa gttcgttatt
    18241 gacatcttta gcaaacttag gattagtact aaatgtgtgt atgagaagaa agaaaataaa
    18301 tttctaaatt taaatacatc taaacataaa ttgaggagat tagatatatg aatatagcat
    18361 tagtagcaca tgaccaaatg aaaaacacta tggtcggatt ttgtataggt tatgaatcaa
    18421 ttttaaaaaa gtatggacta tatgcaactg gaactacagg aaaaagaata atggatgaaa
    18481 ctgaattaaa tataaataga ttagcatctg gtcctttagg tggagaccaa caaataggtt
    18541 ctttaatagt aacacaagaa atagatttag ttattttttt aagagaccca ttgacatctc
    18601 aggctcatga aacagatata caagcgttaa taagactttg tgatgtttat catgttccaa
    18661 tagctacaaa tctagcttca gcagaaatat ttataaaagc tcttgataga ggagaactat
    18721 catggagaga agttagaaaa agcaaaagtc aacgtattta aaaaatatta atattacagt
    18781 ctaaagaaac agatatataa gtgtttaatg ccctagtctt tctgttggaa taaacctagt
    18841 acctgtagaa atcttatgaa agatttaaat gatgaagagc taaatttgag attaaataat
    18901 taggaaatag attatagata gagtagtaga atttaactat tactctattt taataatatg
    18961 aaatagaatc aagtctatta tta
//
LOCUS       DAELJO010000061        18637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELJO010000061 DAELJO010000000
VERSION     DAELJO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.061"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            265..1425
                     /locus_tag="KPB88_001828"
     CDS             265..1425
                     /locus_tag="KPB88_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0054108.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1767..1928
                     /locus_tag="KPB88_001829"
     CDS             1767..1928
                     /locus_tag="KPB88_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054109.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2121..2750
                     /locus_tag="KPB88_001830"
     CDS             2121..2750
                     /locus_tag="KPB88_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG0054110.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2990..4249
                     /locus_tag="KPB88_001831"
     CDS             2990..4249
                     /locus_tag="KPB88_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0054111.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSAVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4508..5503
                     /locus_tag="KPB88_001832"
     CDS             4508..5503
                     /locus_tag="KPB88_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG0054112.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5749..6816
                     /locus_tag="KPB88_001833"
     CDS             5749..6816
                     /locus_tag="KPB88_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG0054113.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7350..9260
                     /locus_tag="KPB88_001834"
     CDS             7350..9260
                     /locus_tag="KPB88_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0054114.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9323..9946)
                     /gene="ytaF"
                     /locus_tag="KPB88_001835"
     CDS             complement(9323..9946)
                     /gene="ytaF"
                     /locus_tag="KPB88_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG0054115.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10134..10778
                     /locus_tag="KPB88_001836"
     CDS             10134..10778
                     /locus_tag="KPB88_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054116.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10992..12050
                     /locus_tag="KPB88_001837"
     CDS             10992..12050
                     /locus_tag="KPB88_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG0054117.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12154..13338
                     /locus_tag="KPB88_001838"
     CDS             12154..13338
                     /locus_tag="KPB88_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0054118.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13545..14258
                     /gene="rpsB"
                     /locus_tag="KPB88_001839"
     CDS             13545..14258
                     /gene="rpsB"
                     /locus_tag="KPB88_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG0054119.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14337..15248
                     /locus_tag="KPB88_001840"
     CDS             14337..15248
                     /locus_tag="KPB88_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG0054120.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15333..16037
                     /locus_tag="KPB88_001841"
     CDS             15333..16037
                     /locus_tag="KPB88_001841"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG0054121.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16063..16620
                     /gene="frr"
                     /locus_tag="KPB88_001842"
     CDS             16063..16620
                     /gene="frr"
                     /locus_tag="KPB88_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG0054122.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16623..16826
                     /locus_tag="KPB88_001843"
     CDS             16623..16826
                     /locus_tag="KPB88_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054123.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16855..17589
                     /locus_tag="KPB88_001844"
     CDS             16855..17589
                     /locus_tag="KPB88_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG0054124.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17602..18381
                     /locus_tag="KPB88_001845"
     CDS             17602..18381
                     /locus_tag="KPB88_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG0054125.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7659 a         1858 c         3234 g         5886 t
ORIGIN      
        1 taaaatattt agtttaatta tctttacaaa taaaaatttt agtaataaaa taattgttga
       61 aaaagatttt aaattcagat tattcatttc taaagtaatt aatgtgcaaa tctttattaa
      121 tatatgtcaa tagttaggta tatatgtatt taattagcat gatatagata aaagtattta
      181 ttttaattgt ctattatctg aaaaagtctt tacttagaaa tcataaaaat caaattaatt
      241 aggaaaagaa agggtgtgta taatgtgatt gagactggat ttgtatattg tagtttcttg
      301 gttgtattcg taggtgtaat tttttttatg gttgataaat tgaaatggaa ggtatttgat
      361 gttattaatc caatgctttt aatatactta ggttcagcta tacttggatc atttggttta
      421 tttgcacaga atgaagatgt tggaacgtat caaaacataa taacagtgaa cttccttcca
      481 ttaatgttag tgtttttgtt aattcaatgt gatttaagaa aaatattaaa gatggggcct
      541 aaaatgttat tatcattttt ctgtgctaca tttacattta tgcttggatt tgttatagca
      601 ttcataattt ttaaaaattc aatgggtgca gattcatgga aaattcttgg ggcagcatct
      661 ggttcatgga taggtggctc atcaaatatg attgcagcag ctaatgctct aggagttgca
      721 gaagaaggac ttagttatgc aatacttatg ggttctatag gatatacagt atggatgtct
      781 gtttgtctaa tgtctgtgaa atttggacct aaatttaaca aatggacaaa gtgcgataca
      841 agttttatag atgaggttac atcaaaactt gaaaaagaaa ataaagataa tacagcacct
      901 acgtttgtag aacttatgat tttactttct gttgggtttg gagttgcgtc ttttgcacaa
      961 attgtatcca agatacttcc aacttcaggt atacttaatg aaactatgtg ggtagtactt
     1021 gttgcatcta cagtatcaat attattgggt atgacaaaaa tatcaaagct taaagggagt
     1081 acactggtag gaaatgtgtt tatgtattta ctccttgcag ctacaggtag taaagcaaat
     1141 ttctttggat tgtcagaagc acctgtttac ataatgtttg gattggtagt agtccttgta
     1201 catgctattt catttatagg gcttgctaaa ctatttaaac ttgatttatt tacttgtgaa
     1261 gtaggaagta tagctaatat aggaggagta actacagctc caatgattgc tggggttcac
     1321 aatcctgtat taataccagt aggtgtactt atggggcttc taggaaatat tatagggact
     1381 tattgtgctc ttatggtaac tcaaatatta catatgttag cttaataaaa atatggtata
     1441 aaattgataa tttaaataag aaaagtcatt tcaatttaag cagaagcttt tactgaaatg
     1501 gcttttttgt ttgacaagat aatctatttc aagaagatgt tttgacatat ttagtgtata
     1561 atattgtaaa atttactaaa aacgagatat atttaatttt tatattgaat cgaacgtctg
     1621 tatgatgtat aataaatata cagaacgaat gtttgtagaa cgggtgtttt ttgaatttat
     1681 tttaataatt tatgaatata tatcagaatg cataaatata tttaaatata aagattaaaa
     1741 agaatttaaa ggagtgttga taacttatga taattccatc aagaagaata caagatataa
     1801 cattaacaaa tttaaaaaat ggtgaagtaa ctttaattga attggatgaa atatacaaga
     1861 agatgggatt tttatttgtt gttagtgaag gcaaattgaa aaagataaaa aaggagaatc
     1921 atcattagaa atgaatgaaa taataaatat tataatagtt tataaaacct aacttatgta
     1981 gaatataaat acttaagtta ggtttttttg tgataaagtt tataagtact caattttata
     2041 agataaataa tggtcttaag tgaaaagtaa ataagtttaa attttagtaa gtattatatt
     2101 gcataagagg tgatattatt atgaaaatag taacctacaa tattcataaa ggtatggatt
     2161 caaataatag attaactcta actaaaatgg ggttatattt aaaacaattg gattgtgatg
     2221 taatttgttt gcaagaagta ttatatcctc aatttttagc tttaaaagct gttttaaata
     2281 tggatggtgt ctttgctaca aatgtaaaaa aagtaaatat gatatatggt atttgtacat
     2341 ttacaacatc taaaatgtta aacagtaatc attttttctt aactagcaaa aaagaacaac
     2401 ggggagctct ttgtataact atagatgcat atggaagaat tataaatgta ataaatactc
     2461 acttgggact tgataggcag gagagagcaa aacaattaga cgaaataata gactatagga
     2521 acagattagt ggggatagtt attttatgtg gtgattttaa tgagaaaaat gtctttttaa
     2581 gtatgtttaa tgatatggct atttcactaa acaagtctta cttaccaaca tttgaaaaat
     2641 caaactctag aatagattac atttttgtta ataaaaatac tgaactaaaa ggatacacag
     2701 tagaaaaaat ttacctgtca gaccattatc ctgtaatagg ttacatttaa atttcaatat
     2761 aaatgaaatt agttgttata aaataaacaa tttttttgtt aagaaatagt aaaagaaata
     2821 taataaaaaa tatcaaaaat aaaactaatt ataagacata aatttacaat aaagttacat
     2881 ttgattaaat tccttgttta acaaaagcta ttcaaatata atctaaggta gttttgaaat
     2941 gggaactcta ttatgcaaat ttaagctagt gttaaaatga ggtgaagtta tgggcagaag
     3001 aacaacaaga gtttcaagat taggtaaaga aaaaaaacga agtgttttaa ttgtattggc
     3061 agctttagtt ctaataatgg gtggatttac ttattttttt aacagtaaat ttctgtacaa
     3121 tgggaaaatc gctaaaaatg tatatataga aggagtaaat gtatctgata tgactaaagc
     3181 agaggcatta aaagctataa cagataagta tactccagaa gacttaaatc ttgcttatga
     3241 tgggaaaaaa tatacaatat caccaaaaga tattgattta aaatatgata cagaagatgt
     3301 tgtaaaagat gcttatgaaa gtacaaaaaa agggtcctat tttcaaaact taaaaaaata
     3361 tattgatata agagtgaata aggcaaatat gaagataaaa gctgaatatg atgaggcaaa
     3421 acttagttcc aaagtgtctt ctatagcaga tagtataaat gttaagatga aaaatgcttc
     3481 tattagtgtt ggaagtggtg gactaagtta tacagactct gctgtaggta gagaatttga
     3541 tttggcagca aataaagagt ctatatataa tatgataaaa aataaagagc ataaaacttt
     3601 agaacttaaa gtaaatcttc aaaaaccaga tataacaact gaacaagtaa aaactgtaaa
     3661 ttctgttata ggtcaatatt caactactta ttcacaagct gtagagggta ggtcatataa
     3721 tgtaggatta tcagcaagaa aaactagtga tgtattatta atgccaggtg aagaattttc
     3781 atacaataaa cttacaggac ctagtaataa agcaaatggt tacaaagatg cacctgttat
     3841 agtttatggt aagctagaac aatcagcagg aggaggtgtt tgtcaaacat cttcaacagt
     3901 ttataatgcg gctcttcttt cgggtatgga gataactcaa gttacaaatc attcatctgc
     3961 atctacttat gtgcctaaag ggagagatgc tactgtgagc gatggaggat taaacttaaa
     4021 gtttaaaaat ccatataagc atcctgtata tattaaaaat tatgcaggtg gaggaagtgt
     4081 atcatcagta atctatggta attctggtga caaaccaaat atatctatag aggtaaaaca
     4141 aactggtcag aataaatatt ctacatatcg aatatttaaa gattcaagtg gaaaggtcat
     4201 aaaaaaagaa catatatcaa atagttctta taaagaatta aaaaaataga taatttttta
     4261 gagaaatatt tgtaatataa aagggagtgt ctcaaaatga actttttagt ttatgaggca
     4321 ctctttttct gtgaaatcaa atatattttc atcatttcaa caatacaaaa gaagcgtatc
     4381 tctattttga gacagccttt tttgtaaaaa ttaacactat tagttcttgt tttaatatta
     4441 caatcattaa tatatagaaa atattcctat atattggtat aattgtaaat agatttagtt
     4501 atataaaatg tatgaggagg tgacatggtt gaattttata ggcaatagat atgaggtagt
     4561 aaattctgac gacgttttag aaattaacaa aatctataaa gctagagatg ttttttacag
     4621 gaaaaatgtg ttaataaaag ttataaagca caataattat atctgtgaag attttgtttc
     4681 aaatttaatt gatgaaagta ctgctttaga tgagataaac tctccttata tactaaaaat
     4741 aatagatgtt gggattcact gtacagaaga aacaacatta tattatgttg taagtgaaga
     4801 ctttaatggt atcggactag atgaattaat tctaggtaat tatcttcatt tagaagcaat
     4861 tgtaaacata atgatacaag ttttaaaagc tttagaaatg atacatagga atcattcata
     4921 tcatggtagt ttaaaatcga gtagtataat agtagataca gaatacaata taaaaatttg
     4981 tgattttgga attacaaaag caaataatgg ggttaataca agaagctatg gaaacaggag
     5041 atatttatgc ccacatcaac tatgtataaa ttatactgat aaagaaagtg atttttttgc
     5101 aacaggtctt attctatttg aatctatatt taaaaaattg ccttttggag aatcaaattc
     5161 agaagaaaaa atgctacaat ctattgataa gggattagat tggaatagta taaaagcaat
     5221 taatggaaat actgaactta taaatgttat aaaaaggctt cttggaagaa ataataagta
     5281 taccagagcc aatgatatta ttattgattt aagtaaagta atgtatgaaa aagcatatat
     5341 agaagttgaa gatgagaaag aagaagagca gaaacaagac aaagttatac aagttaaaaa
     5401 agaatataaa agtaaaatga gaaaaaaatt acataaaaaa cttgttgttg ccggtctagc
     5461 tatagtcatg atttcaatga ttattattag ttccataata taaaaattgt atattaagta
     5521 ttttaaaatc aatgtctata aaggatatac atagaaattt tagtgtagtc ttaaattatg
     5581 aaaggaagtg gttattatgt taacaaaaaa taatgaggta ttaaacgtct aggagtattt
     5641 taacaaatag ccacctgctt ttacagttta gaataaaaaa gctgtggcta aaggtcacag
     5701 ctttttgcta ctttttaaag gcaggagaat attaaaaagg agcaaattat gagcaatata
     5761 aaaataatag caagtaataa atgttggatt gaagatatag caaggaaaca acttgaagat
     5821 atttcaaagt taaatggaat tttaagagta gtaggattac ctgacttaca tgcaggtaaa
     5881 atacctgtag gtttagcagt tgagactaaa aatattattt atccacatat aattggaaat
     5941 gatataggat gcggaatgac tttatttaaa actggggttc taaaaaagaa atttaaaaaa
     6001 gacaaatgga ttaagtcact tagtaagatt aaagatttgt ctgatataga aataaaaaac
     6061 acatataaag aagaatgtcc aattttaaat ttaggaacta tagggagtgg aaatcatttt
     6121 gtagaaattc aatgtataag tgaaatttat aataaagaac aatttgaaca attaaaattt
     6181 tcatctgatg atattatgat gttagttcac tgtgggtcaa gaaattatgg agaagatatt
     6241 ttaaaaaaat tttatgataa agatggactt gaagttgaaa gtgagaaagc tatagattat
     6301 attaaaaaac atgataatgc tttaatgtgg gcagagcgta atcgtgaagc tatcagtaaa
     6361 aaaattatgc agtcaatagg tacatctgga gatgttgaaa caatattctc tataaatcat
     6421 aactttgtag agaaaaggga agataaattt atacatagaa aaggtgctgt ttcaagtgaa
     6481 agaggagctg tgattatacc aggttcaaga ggcagtctat catatatagt tatgcctact
     6541 gaaaatacag agatatcctt atattctcta tcacatggag caggtaggaa atggtctcgt
     6601 tcagtttgta aatctcgcct taaaagtaag tatagtaaag atactataaa acaaactaaa
     6661 tttaaaagtc agattatatg taatgatgta aatttattat ttcaagaagc tccagaggca
     6721 tataagaata tagagcagat tattgaaagt ttgatagaat atgaacttat tcaagtagtg
     6781 gcaactatga agccattgat tacttataaa ggataatttt taaagttaaa aaagattaaa
     6841 attatattat aaatagctaa ttttatggaa catttaaaag tgttttatta ttagctattt
     6901 ttttattttg tagagacgat aatttataga gctaaattct ttgatatata atgattacaa
     6961 agttagactg tattggaaac tgcacataca tgaattgatg acaatataag tgattccaaa
     7021 atataaatga aagtataagc ttattttcac tataaaaatt ttcactgtac aaaaacatta
     7081 atgacattat ttttattgac ataaatatat aatttatgga ataatattag aagcactgat
     7141 ataagtgttt aaataagaat agaaaaggag aagtgtttta tgaaaaaaaa tactaacatt
     7201 aatgagtccg gagttaaact tcttaataaa taaggagttt gatatataat aactccttat
     7261 ttattaagtt gttcgcaaaa taactattat caaagtaaat gatagggtga tttaaaagac
     7321 gataacttat aaataaagga gaaaaaataa tgaatagtaa tacatttgaa agattacaac
     7381 taaatgaagt taaagactta ataaaaatac attgtgtaag ttcactagga aaaaatttaa
     7441 tagataaatt aactcctagt gggaatattg gagtagtaag aaggaaatta gcagaaaata
     7501 aagaagctag aaagataatt gaaaatagta atcacatacc acttgaagga ttgtttaatg
     7561 caggttctac aatagataaa attgaaaaag gaatgataat agaacctgtt gaacttgtaa
     7621 atattgaaga ttttttaaga ggttgtagga agatgaaagc ttttatgtta gaaaaagaat
     7681 tttattcacc tacattaagt tcttatgcct taaatataac agaatgtaaa agtatagaag
     7741 atgagataaa ttactgtata aaatctaata aagttgattc taatgctagt aaagaattaa
     7801 aaaagataag acgaaatata gaaattacag aaggaaagat aaaagataga ttaaataaat
     7861 ttataacatc aactgtaaat aaaaagtata tacaagagtt tataataagt aaaagaaatg
     7921 atagatatgt aataccaatt aaatcttctt ataaaaatga agttaatggt actatattag
     7981 atacatcatc aaaaggaaat actgtattta ttgaacctat tagtgtttca aatttaagta
     8041 cagagttaac tatgctaaaa gctgatgaaa ctattgaaga atacaaaata ttatcttatt
     8101 taacagaact tatatttgag aagataagtc agataaaatt aaatatagag atactttcag
     8161 aatacgatat ggtatttgca aaagctaagt atagtcaaaa aataaaagga attactccaa
     8221 aaataaataa taatggctat ataaaaataa ttaaaggtaa acatccactt ctaacaggag
     8281 atgcagtacc tttagatttt gaaataggca aaaattatag aagtcttata ataacaggtc
     8341 ctaatgcagg aggaaaaact gtaaccctta agactgttgg tttactaact ttaatggttc
     8401 aatgtggatt tgatatttct gcaaaagaag gtagtgaatt tagtgtattt gaaaaagttt
     8461 ttgtagatat aggggataat caaagtattg aaaatgcctt aagtactttt tcatcacata
     8521 taaaaaatat tgcagagatt atgagtttat caaataattc tactcttgtt ctgtttgatg
     8581 aaataggaag tggaactgaa cctaatgaag gagctggttt agcaatatca ttattagaag
     8641 agttttacaa aatgggatgt ataactatag catcaactca ttatggagag ataaaaaagt
     8701 ttgctacatt gcatccagaa tttgaaaatg ctggtatgat gtttgataaa gaaacgttag
     8761 aaccattgta taagttaact attggaaaat ctgaagatag taatgctctt tttatttcta
     8821 aaaaaatggg aataaaaaat agagttttag atagagctaa agagtatgta attgatagaa
     8881 attacaattt agatttagtt aaaagaagta agctacaatc taatattgaa gataaaaata
     8941 gttctgaagt cattttaaac tatacagatt ataatgtagg ggataaggta aaattactag
     9001 atgaagatga ttttggaatt gtatataagc ctatggacaa gtttaataat gtagaagtat
     9061 tgtttaatga taattttatt tcagtaaata taaaaagatt acaacttagc ataaaagcat
     9121 cagaattgta tccagaaggg tatgatttag atgtattatt tactagtttt aaagaaagaa
     9181 aattagaaaa agatattcaa agaggttcaa aaaaagctct taaaaagatt cagaaagaaa
     9241 ttagagagaa tagaaaataa atataaaaag cacaaaagaa agcaatctaa agagaaaagt
     9301 ttatctttag attgctttct tattaacata tcttcataaa tccaatgact aacaagatta
     9361 aaccacttaa ccaagataag tttaaattta cgtttttaac aaacatattt cctagtaaac
     9421 aaccaagtaa tactgcaatt atatttgata ttaaagaaaa tagaataact tgtatataat
     9481 tcacacctac aaggccacta ccaaaaccga tagctaatcc atctaagctt aaagcaagtg
     9541 caagtgtaaa tgcttcagca gatttaagca ctttagattt acttgtatct aagacatcag
     9601 gttcgatact tacattaaaa tttaatttaa ggtctaacat attaaaatta taattctttg
     9661 agtgtataga ttttttctta aaataagatt taaaaaaact ttcaattagt ctagcacttc
     9721 ctaataaaaa taaaataaag aaacatatta acattgttat attttgaggt aaaaagtctc
     9781 taactattgt acctgcaaaa agcgatattg ctaaaatagc tgaacaaact acgcttataa
     9841 ttatatttga atataaggga actttaactt tatttacgcc atatccaaag cttgtcacaa
     9901 aggcatctat acaaagagaa gtaactaata aaattgattc aatcacagta gattaacatc
     9961 ctttctctta aattttgtac ttatattagt tatagtattg taaaaatgga taaattgtta
    10021 cagataatcc aataaattgt tgataaaatt ccaacatcat acaacaccta aataacaaaa
    10081 gaatcatatc atataataat ataaaaccaa tcataataaa gcgggtgaaa ataatggaac
    10141 aaaataataa ggttgggaat ttcggtgatt tgttgaagat tttattagat gaaaaagaat
    10201 tatcaatggg agaactaagt aaaaaaagtg gaattgataa atctactata tctcgtatag
    10261 ctaataataa acaaaagcca aatataaatc atttagaaaa gatggcaata catttaaata
    10321 ttaacctaga agaactgttg aaagcttcag gatatgaatt tagaaacagc aataatcaaa
    10381 tatttaatgc agatagtgat tttagtaact ttgatgatat tcttggattt gcaaatttaa
    10441 tcaatgataa gaactttaat ggaaatattg aaaaagagtt aagtaagtgt aagttatatg
    10501 tccaaacaga tgaaggtaaa aaattacttt ttgataattt taataaaaaa atagattcta
    10561 ttgaaaaaca aggtcaattt acagatagat taagacaaat gtatgctgat ttttgtacag
    10621 atggacttac aataaagaaa tatttattga ttggaagtat gctactttac tttgttatat
    10681 caacagatgt gattccagac ttcgtgttcc caataggatt tatggatgat ttagtagcat
    10741 taaatatagt tacaaaatta cttaaaaatg ataagtaagt tttagaatca tatttaatat
    10801 ataaataaat caaagtatat aaaatttatt attttttata tgaaacaaat taagagcaca
    10861 atgaaataga ttaaaaattt aaaactaaga atctaatata tattttgtaa ctatttaagt
    10921 atataaaata aaataatatc caaactaata caaaatagta ataattgttt tgtactggag
    10981 gatatatatt tatgaattgt aaatggatat ctaaaataga tgagagaaaa aaatgtcata
    11041 gagaagcaga ttcttctgga tattgtatct ttcataaaga aaataagtct gatgaagaaa
    11101 tacaattgat gatggatact attcataaag aagagataag tgaatttaat ggatttgttt
    11161 ttgaaaatga atttaatgcg gaagaaatat taacttacaa ttataaaata ctggattttt
    11221 ctgaatccat atttaaacaa aaagcaaatt ttaaaaaata tatttttaaa aagaatatta
    11281 tttttaacta tacagaattt agagataaag ttttatttaa tggttgtgta ttcttagaaa
    11341 actgtgattt taatagaagc atatttagta aaaactatat aaatgaccgt atatttgaaa
    11401 aagtgaaatt taaaggacct gatttggtag ttaataaagt agaaaatttt ccacgaatgg
    11461 atggaataat ttttagcatg tgtacaaaat ttgtgttaaa gaatgttgaa tatggaaaaa
    11521 gtgagtatga acatggcaag ataaattata gaatagctag aaatcaagca acaaaaattg
    11581 gagaatatga gatgatagga ttttactatt ataaggaaag aatttatagt agtaaaataa
    11641 tgaaacgttc taattatcct actttcagtg attatttagt tgaaaaattt tttgaccaaa
    11701 tagcacgtta cacaacaggt tatggagaaa agccatggaa tatattatta gttattatag
    11761 caataataag tgtttttgct ttattatact tatttgtagg catagaaagt tcaaatagca
    11821 cacttgtagc tttagatata aacaatatag gtgattactc actaagtgag attttcaaaa
    11881 tgtatatgga tttatggtat tttagcatgg ctacatttag tacagtagga tatggagata
    11941 tggttgctac aagtttgatt gggaaagcat tggcaggtat agaagtattt tttggagtta
    12001 caataggagc aatttgggca tctgtgatta ttaaaagaat gataaggtaa tatttgtgta
    12061 aatatggata ggtattaatt actagatata aattggacta aatagggaaa atttaaatat
    12121 aaatatactt aataatccaa gaaggtgaaa aatttgaaaa atgcactaaa aaaaattata
    12181 tcattagcca tgattctagt ttttataata ccaaattatt catatgcaga tgataaatct
    12241 gtagacaagt attcaaaaac atctatactt atagaccaag aaacaggtag agttttatat
    12301 agtaaagaac ctgatatgaa ggtaccatta gctagtttaa gtaagatgat gactttttta
    12361 cttgcaatag aagctattga aaataaagaa gtaaaagaaa ctgataaagt aaaaatagac
    12421 aaatctattg catcagtaaa agggtcaagt tataaactta aagatggtga agaagtgcca
    12481 cttatagagc ttatgagagg tcttatgatt gtatcaggaa atgatgcggc aattgctata
    12541 gcaaaacata tttgtaaaac gaaagaagca tttgtagata gaatgaataa aaaagcaaaa
    12601 gaaataggaa tgagtaatac acattttcta aatccaaatg gtcttccaat atacgattta
    12661 agcaacccca aaaaaccagc aaaagaaaat atctctactg caaaagatat agctattctt
    12721 ggaaaataca tgtttgacca ttatgaaaaa caagtaacag ctataactga tatggagaca
    12781 tatacaaatt cagatagaag ttttgaaaaa agcaatacaa atgcattatt aagaattata
    12841 ccagaagtag atggtataaa aactggatat actggcaatg ctggatattg cttatcattt
    12901 tctatggttg taaataaaaa tgataaaaat gagaaaaaac gtagagtaat aggggttgta
    12961 ttaggtacta accataaaaa taaaagaacc tctgcttcat tagctctact gaaatatgga
    13021 aaagaaaatt tcaatatgaa aaaagtaatt gataaagata gctttattgg taagaaatat
    13081 ataaaaggaa tgaaagagtt agaggttaca ttaaaagcaa aagatgaact ttatacaata
    13141 ttaaaagatg atgaaagtat aaaatcagaa gttaagatta aaaatataga gtaccctgtt
    13201 aaaaaaggtg atactatggg tattataaaa tattatacta attcaggtga cttacttgga
    13261 agtgtagata tagtaagtaa taatagtata gataacgtat cattaaaaac aaaattaaaa
    13321 atgaaatttg caaattaaat tcaaaaaatg gtataaaata cacaaaaaaa tataaaaata
    13381 gaaaatatgg aaaaaatatt gttatttctt cacctctgtg atatactact agaggtgaaa
    13441 aaataaaata aatcacacac agcattggga tccttaaaag gtgcctatta catggttttt
    13501 tagggagaag atcaagctgg aggtataaaa ccaggaggta agatatgtca gtaatatcaa
    13561 tgaaacaatt attagaagca ggtgttcact ttggccacca aacaagaaga tggaacccta
    13621 aaatggctaa gtatatattc acagagagaa atggaatata tataatcgac ttacaaaaaa
    13681 cagttaaaaa agttgaagaa gcttacaagt tcactaaaga agtagctgaa acaggaaagc
    13741 caatcttatt cgtaggaact aaaaaacaag ctcaagatgc gataaaagat gaagctgaaa
    13801 gatgtggaat gtactttgtt aatgaaagat ggttaggtgg aatgttaaca aaccacaaaa
    13861 ctataaaaac aagaataaat aaattaagag aattagaaaa aatggaagaa gaaggcgtat
    13921 ttaatgttct tcctaaaaaa gaagttataa aattaagagc tgaaaaagaa aaactagaaa
    13981 aatacttagg tggaataaaa gacatgcctg aattaccagg tgcaatgttt gtagttgacc
    14041 caagaaaaga aaacatagct attcaagaag ctcatagatt aggtatacca gtagttggta
    14101 tagttgatac taactgtgac ccagaacaat tagactttgc tataccagga aatgatgacg
    14161 ctataagagc cgtaaaatta ataactggtg ctatggcaac agctgtaatt gaaggtagac
    14221 aaggtgctga ggaagaagta gctgaagacc aagaataata atttgacaat taaatggtaa
    14281 gggtcgccct taccatttat aatatggaat agaataagta ggaggaaact taaaaaatgg
    14341 ctaatataac tgctcaaatg gtaaaagagt taagagaaag tacaggcgca ggaatgatgg
    14401 actgcaagaa agctttacaa gaagctgaag gaaacatgga aaaagcagta gatttattaa
    14461 gagaaaaagg attatcaaaa gctgctaaaa aagctggtag agttgctgct gaaggtctag
    14521 ttgcaataga aatgaatgat gataatacag ttgcttctat ggtagaagtt aactcagaga
    14581 cagatttcgt tgctaaaaac gaagacttca aagtgttcgt taaagatgct gcttgtatgg
    14641 cattagctac tgataaagaa gatatagctt ctttattagg tgaaactcat aaagagggaa
    14701 taactttaca agaagtttta aataatagag ttgcaaaaat aggtgagaaa ttagacttta
    14761 gaagatttgc taaagttgta actaatggac aagttgctgg atacattcac ggtggtggaa
    14821 aaataggtgt tcttgttgaa atggaaacag aagctagaga tgctaaagtt ttagaattag
    14881 gtaaagatgt agctatgcaa gttgctgcta tgaatcctaa gtatgtttca agagatgaag
    14941 tagatgctga atacatagca catgaaacag aagtattaac tcaacaagct ttaaatgaag
    15001 gaaaaccagc taatatagtt gaaaaaatgg ttaaaggaag attagaaaaa gaattaaaag
    15061 aggtttgttt attagagcaa actttcgtta aaaatccaga tataactgtt aaacaattag
    15121 ttgctgatgt tgctaaggca gtaggttctg atataaaagt tgttaaagtt gtgagattcg
    15181 aagttggtga aggtatacaa aagagagaag aaaactttgc agaagaagtt gctaagcaac
    15241 ttaagtaata accaaagaga gaacacgcag gtgttctctt ttttaaaaaa tagaaaaaat
    15301 tggatatgaa ggattgaaat aggaggtttc tgatggataa accaatgtac aaaagggtat
    15361 tgttaaaatt aagtggagaa gcattagcag gagaaagagg ttttggtata aataatgatg
    15421 tagttaatga tattgctatt gctataaaaa aaatacaaga aataggtgtt gaagtagctg
    15481 tagtagtagg tggaggaaac ttctggagag gcagaaccag tgaaggaatg gacagaacta
    15541 ctgctgacta tataggaatg ttagctacag ttatgaatgc aatggcgcta caggatgcac
    15601 tagaaaatat agatgttgct acaagagttc aaactgctat agacatgaga caaattgcag
    15661 aaccatatat aagaagaaga gcagttagac acttagaaaa ggaaagagtt gttatatttg
    15721 gtgcaggaac aggaaaccct tactttacaa cagatacaac tgctgcttta cgtgctgctg
    15781 agatggaagc agaagttata ttgttagcta aaaatgttga tgcagtatat gacaaggatc
    15841 caaaagtaca tgcagatgct aagaaattta cagaattaag ttacatggaa gttatacaga
    15901 aagaattaaa agtaatggat tctacagcta catctttatg tatggataat aaaattccaa
    15961 taaaggtatt tgagcttaca acagagaata taataagagc agtaaaaggc gaaaatatag
    16021 gaactactgt aaaataatga agaatgaagg aggagtaaaa ttatgaaact ggaaatacat
    16081 aagcaattag aagagaaaat gaatgggaca attgatgctt taaagtttga atttggaact
    16141 ataagagcag gaagagctaa tgcacaaatg ctagacaaaa taagagttga ttattatgga
    16201 actccaactc caataaatca aataggtgct atatctgtac cagaaccaag aatattaatg
    16261 atatctcctt gggataaatc tgctatgcat gaaatagaaa aagcgatagc taactcagac
    16321 ttaggattaa atccatcaaa tgatggtgag gttataagac tttctgtacc tgctttaaca
    16381 gaagaaagaa gaaaagaatt agctaaaaaa gctagtaaag ctgcagaaga atttaaagtt
    16441 agaataagaa atgaaagaag agacgccaat gagaagatta aaaagatgga aaaaggcgga
    16501 gaattaactg aagatgaatt aaaaaaagca caagatgaag ttcaaaaaat gactgataaa
    16561 tttataaaag aaatagatac attattatct aaaaaagaaa aggatataat ggaggtataa
    16621 tattgaaaaa atattataac cttttaggtc ttcatgtaga tgaggtaaaa caatattttg
    16681 atgagcaaaa tataaattat accgtaaaat ctattgaagg taaaaaggat aaagaaaaac
    16741 ttgtagttcc tagagttata aaaatatctg agataggtga tagtgtagaa ctgataatca
    16801 catacttttc tgactcctta gtttaaggag tcagaacaat attggaggca aaatatgaat
    16861 aacaacatta tatatgatat agacttaaat aacataccta ctcatattgc tattataatg
    16921 gatggaaatg gaagatgggc aaaagctaga tttcttccaa gaactgcagg acataaggct
    16981 ggagtagaaa ctataagaga tatagtaaaa gaatgctcaa aattaggtgt taagcactta
    17041 acattatatg cattttcaac agaaaactgg aaaagaccga aacttgaagt tgacacactt
    17101 atgaacttat tatctacata cttaagaaat gaaatagctg aacttcacca aaacaatgta
    17161 aaagtaactg ctataggaga tataagtgct ttaccaaaaa cttgtataag agaattaaac
    17221 tcagctaagg aactgacaaa aaacaataca ggagttaact taaatttagc attaaactac
    17281 ggaagtagag cagatataaa aaatgctctt atagatatag taaaaaattg tgaaagtggt
    17341 aaaatagata taaacaatat tgatgaagat attataaaaa actatttaag cacaaagtct
    17401 ataccagatc cagacttggt aataagaaca agtggtgaac aaagattgag taacttttta
    17461 ctttgggaag ttgcttattc tgagttttac tttactgata tacattggcc tgattttaaa
    17521 aaagaagaac tacagaaggc tatatacgta tatcaaagaa gggatagaag atttggagga
    17581 cttaagtaag gagattattt tatgcttaca agaattattg cttcactagc tctggttcca
    17641 ttatttctat ttgttgtata tggaggtatt cctctatata tagctgagac agcaatagtt
    17701 tatattgcat tgcatgagtt ttataaggca tttaaaataa aagatgtaca tccaatattc
    17761 attataggat atttattttc tatttattta gctgtaaaaa atatttttaa tttaccatta
    17821 gaatatactt atgcagtaat ttttatttta tttttagcaa gtataattta tatgttgatg
    17881 ggcaaaaaca atgttataga tgtttcgatt acgtttttag gagtttttta tataggagta
    17941 tttttagatt ttataattat aactataaat ggatttgaaa aaggtagtat atatgtatgg
    18001 cttatatttg tgatatcttt tatgacagat atatttgcat attttagtgg ttatttactt
    18061 ggaaaacata agctaattcc taaagttagt cctaaaaaga ctatagaagg agctattgga
    18121 ggaataatag gaagtacact ttgttgtatt ttatttggat atttatttgg cattgatttg
    18181 cttcaattag cgataatagg aagtatagga agtgtaatag ctcaattagg agatttattt
    18241 gcatcttcta taaagagata tgtagggata aaggattatg gaaagataat acctggtcat
    18301 gggggtatat tagatagatt tgatagtgtt atattagttg caccttttgt atatagtgcg
    18361 attaaatttt ttattagata aagattggtt ggatttaaaa gagcaatcta taattgctag
    18421 taatgaaaat agctatgtaa cttttaaatg cagagatatt ctgcagttat tagttacatg
    18481 gctattttca ttgttcaaaa atttagaaga ccttggggaa ttgtataaga ataggtatta
    18541 ctttataata gtatactata tttgtagtga gtatattttt tatactgtaa ctatcattaa
    18601 aaaatatttt tttgtttaat aatttcgata tttttag
//
LOCUS       DAELJO010000062        18571 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELJO010000062 DAELJO010000000
VERSION     DAELJO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.062"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..222
                     /locus_tag="KPB88_001846"
     CDS             <1..222
                     /locus_tag="KPB88_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0054126.1"
                     /translation="YKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIGFGPSKENLA
                     HTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            238..1821
                     /locus_tag="KPB88_001847"
     CDS             238..1821
                     /locus_tag="KPB88_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0054127.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1849..3177
                     /gene="ssnA"
                     /locus_tag="KPB88_001848"
     CDS             1849..3177
                     /gene="ssnA"
                     /locus_tag="KPB88_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG0054128.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3322..4674
                     /locus_tag="KPB88_001849"
     CDS             3322..4674
                     /locus_tag="KPB88_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0054129.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4866..5975
                     /locus_tag="KPB88_001850"
     CDS             4866..5975
                     /locus_tag="KPB88_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0054130.1"
                     /translation="MANLKVNLYGMEFLTPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6258..7649
                     /gene="hydA"
                     /locus_tag="KPB88_001851"
     CDS             6258..7649
                     /gene="hydA"
                     /locus_tag="KPB88_001851"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0054131.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKKRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7643..10204
                     /gene="xdh"
                     /locus_tag="KPB88_001852"
     CDS             7643..10204
                     /gene="xdh"
                     /locus_tag="KPB88_001852"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG0054132.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10356..11702
                     /gene="rpoN"
                     /locus_tag="KPB88_001853"
     CDS             10356..11702
                     /gene="rpoN"
                     /locus_tag="KPB88_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG0054133.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12500..13522
                     /locus_tag="KPB88_001854"
     CDS             12500..13522
                     /locus_tag="KPB88_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0054134.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13582..14589
                     /gene="gap"
                     /locus_tag="KPB88_001855"
     CDS             13582..14589
                     /gene="gap"
                     /locus_tag="KPB88_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0054135.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14811..16013
                     /locus_tag="KPB88_001856"
     CDS             14811..16013
                     /locus_tag="KPB88_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG0054136.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16039..16782
                     /locus_tag="KPB88_001857"
     CDS             16039..16782
                     /locus_tag="KPB88_001857"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG0054137.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16791..18323
                     /locus_tag="KPB88_001858"
     CDS             16791..18323
                     /locus_tag="KPB88_001858"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG0054138.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDLFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQVSMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7450 a         2145 c         3734 g         5242 t
ORIGIN      
        1 tataagggat taaaagaagc tggaatagat tcagaaataa ctcaatactc attctgtacg
       61 aatggaagtc actacgcagg tgaggctgga ataagaacta taggatttgg tccttctaag
      121 gaaaatcttg ctcatacaat agatgagtat atagaattag accaattatt caaaggtgca
      181 gaaggatatt atggtatatt aaaatcagtt tataaaaaat aattaggaga tggttttgtg
      241 agaactttaa taaaacatgg gcttatagta gatggaaata aaacaccagc atatgaagga
      301 gatattttaa tagaaaacga gaaaatatta aaaatatcac aagatttaaa cgaagaagct
      361 gacaaagtta tagatgcaaa aggtagagtt atatgtccag gtttcataga tactcatagc
      421 cattcagacc tagttatttt agttaatcca tataatgagg taaagattag acagggaatt
      481 acaacagaag tgttgggcca agatggtata tctatggccc ctctacctca agagcatata
      541 tcttcttgga gaaaaaattt agcaggttta gatggtgaaa gtgatgaaat tgattggaaa
      601 tatgaaacaa cagagaatta tttaaaaatg atggattaca atggggtagg tttaaatgaa
      661 acttatttag tacctcatgg aaacgttaga atggaagcta tgggacttga agatagacct
      721 gctactaaag aagaaattca aaaaatgtgt gaaatcacag agagagaatt aaaagcagga
      781 gctataggat tgtctactgg acttatatat ataccttgtg catactcatt gactgaagaa
      841 attatagaga tgtgtaaggt agtagcaaaa tatgacggag tatttgtagt tcaccaaaga
      901 agtgaagcag atacaatttt aacttcaatg gaagaaatca tagaaattgg aaaacaatca
      961 ggagtaaaag ttcatttttc acattttaag gtttgtggta aagcaaactg gaagtatata
     1021 ccacaagtta tagagctttt agaaaaagca gaaaaggaag gaataagagt atcttttgac
     1081 caatatccat atgcagcagg aagtacaatg cttggagtag ttttacctcc ttgggcacat
     1141 tctggaggaa ctgataagtt aatagaaaga ttaagtgatg aaaacgaaag agcaaaaatg
     1201 aaaaaagata tagcaaatgg aatagaaggc tgggataatt ttatagaatt tgctggaata
     1261 gaccaaatat ttgtaacaag cgtaaaaaca gaaaagaata aagatacaat tggtaagagc
     1321 ttattagaaa ttggaaaaat gagaggaaaa gacccattag atgcaacgtt tgatttatta
     1381 aaagaagaag aaaatgcagt tgggatggta gatttctatg gattagaaga acatataata
     1441 ggattcatga aaagagacga gcaaaatgtg tgtacagatg gtcttttagc aggtaaacca
     1501 catccaagag cttatggttc tttccctaaa atactaggaa gatatgttag agagttgaat
     1561 gtgttaacaa tcgaagaagc tgtttataaa atgactaaaa aggcagctac aagctttagt
     1621 ataaaagata gaggcgaatt aaaagaagga tattttgcag atatagttat atttgacaag
     1681 gatacagtta gtggttgtga tgattttatc aactctatgc agtaccctac aggtatagat
     1741 tacgtaatta taaatggaaa ttgtgttgtt gaagaaggaa aatacaataa aattaaagca
     1801 ggaaaagtac ttaaaaatta aatcttagct ttaaggagga ataaatttat gattttagtg
     1861 gggaatggga ggctaataag ccaagattct ttaaatccct atatggaaga tggatgtgta
     1921 gtaataaatg ataatctaat tgaagatata ggtactacag aagaaatgaa agctaaatat
     1981 ccagtacatg aatttataga tgcaggcaag aaaataatca tgccgggtct aataaattca
     2041 catatgcata tatacagctc atttgctaga ggaatggcag ttccaggaaa accttctgag
     2101 aactttttag agatactaaa caatttatgg tggagacttg acaaacagct tacactggaa
     2161 gatacaaaat acagtgcata tgcaacgtat atagaatgta taaaaaatgg tgtaacaaca
     2221 gtttatgacc atcatgcaag tccaaatgca attgaaggca gtttgtttac aatatcagat
     2281 gttgcaaagg atttgggaat tagaacgtgt ctttgttatg aagtgtctga tagagatggt
     2341 ggaaatacaa tccaacaagg tatcaatgaa aacgtaaact ttataaaata cgctcaaaaa
     2401 gatgatacag acatgataaa aggaatgttt gggcttcatg ctgcatttac actatctgat
     2461 gatacattaa ataaatgtgc ttcagagatg gctggtcttg atacaggtta ccatgtacat
     2521 gttgctgaag gactagcaga cttacaatat aatgtagata aatatggaaa aagggtttta
     2581 gaaagattca atgattttgg agtactaaaa gaaaaaacaa tagcagttca ttgtatatat
     2641 ataagtgcag aagaaatcag tcttttaaat aaaacaaata catttgtagt tcataatcca
     2701 gaatctaata tgggaaatgc agttggatgt tctcctgcaa tagagttatt aaaaagggga
     2761 gttacagtag gattaggaac tgatggatat actagcgata tgtttgaatc attaaaagtt
     2821 gccaatataa ttcacaaaca tcacttaaaa gacccaagag ttggatttga agaatcacaa
     2881 acaatgttat ttgataacaa tagaaaaata gctaaagctt attataaaaa tgacctagga
     2941 gtacttaaaa aaggtgctta tgcagatgtt atagtagttg attatatacc acatactcca
     3001 ttgacagaaa atacaatagg gggacacact atttttggaa tgagtggaag aagcgtagat
     3061 acaactatta taaatggaaa ggtacttatg aaagatagaa agttaataaa catagatgaa
     3121 gaaagtattc tagcaaaaag tagagaatta agtcaaaatc tatggaatag aatttaaata
     3181 attgatatta atatacaagt aagaaacaag aatttagatg ttaaaaacag agcttaaaca
     3241 aaaataggaa tttaaatgaa gtatataaat aaaaaatata cggaatagaa tattaaatta
     3301 aatatagaag ggagacccaa aatgtctcga gataaaagcc ctaaaatagc tccagtagat
     3361 gaaaggttac ctgtatccaa atcttgggta ttttcactac agcacgtttt agcaatgtgt
     3421 gcaggagcag tagcagttcc tatgatgata ggaagtgctg cacgactaga tcatgctagt
     3481 attgtttttt taataaatgc ttgcttgttt atggcaggta taggaacact catacaaagt
     3541 gttggtatag gaaatattgt aggtgctaaa ataccagtaa tagaaggaac tagttttgcc
     3601 gctgtggcag gtatgacagc aatagcctca tcatatagtg attcacaagt tgcaatacag
     3661 acagtatttg gctctgtaat agtagctgga ttgttttgta tattacttgc tccagtattt
     3721 gaaaaattaa taagattttt ccctagtgta gttactggaa cagttgttat ggtaataggt
     3781 atttcactat ttccagttgg gataaaatgg ataactgaca acaaattgga agcggttgaa
     3841 ccaaactcat tagcattagc tttagcagta ttggcaataa ctttactttc atttaaattc
     3901 ttaaaaggaa tttggaatag cacagctata ttggtaggta tagtacttgg tacactactt
     3961 tccatggcag ttggaatggc agattttagt gcagtaaatg gagttggttg gcttaactta
     4021 aatgtacctt taaaatttgg aatgcctaga tttgaaccat ctgcaatact atcattgtgt
     4081 ttgattatgt tggtacttat gacggaatca gttggtaata tgatagctat tcatgaaatg
     4141 gtagacaaag aagtaacagg taaaaatatt aaaagaggtc tatttggaga tggaatatct
     4201 acagcattat ctggaatatt caattcattt ccaataactc catttggtca aaatacagga
     4261 atagtaggtc ttacaggtat aaaatcaaga tttatagcag tttacgcagg ggttatattg
     4321 ctagtacttg gattcttacc aaaatttgca gcaataatag gagcaatacc taagcctgtt
     4381 cttggaggcg taggatttgc aatgtttgga atggtaatgg ttggaggagt aaaaacttta
     4441 agcaaagtga aatttgatgg aactaaaaat ggggttatag tagcagttag tgttggtttg
     4501 tctatgatac cactaacaaa tcctgatttc tatcataatt tcccaacttg ggtacaaaca
     4561 atattccata gtggaataac aacaggtagt ttatcagcag tgcttttaaa catattcttc
     4621 aatgaactag gttctaaaaa agaaaaaaat aataaagttg aagaagcaaa ataaaaataa
     4681 aacaagtaaa aatctatatt gacttaaagt cattaagtat taataaaaat aattcagaga
     4741 tattaataat tcaacaaaac tatcttatat tttaaataat ataaaaaagt aaataaataa
     4801 cgaatacaaa taaaagaata caatctaaat attaaaaata tttaatttaa gggagagata
     4861 ataatatggc taatttaaaa gttaatttat atgggatgga atttttaacc ccaattatga
     4921 cagcggcagg acctggagca aaagatggtg ctctctgtca agaggcagca aaaggtggag
     4981 caggagctat atgtacaaaa actatatctg tattaccagc tgatgtacca agaccatgta
     5041 tggcaaaaac taatagtggt tttttaaaca cagaattatg gtcagagctt ccaaaagagc
     5101 aatggataga ggaagaatac aaaaaggtaa aagaaacagg gcttccagta atagtaagta
     5161 tgggatattc agcagagcaa atagctgagg ttgctccttt agtaaagcct tatgcagatg
     5221 cagttgaatt atctactcat tatgtaggta cagacgttgc accaatagta aatgctttaa
     5281 aagctgcaaa agctgtttta gatgtaccag tatttatgaa aatgagtcct catactgaca
     5341 ttcaaactat tgcaaaagct gttgaagaag caggtgcaga tggacttgta atgataaact
     5401 catttggacc atgtatggca atagatgtaa atacaggata ccctataatg ggaagtaaag
     5461 ctggatacgg atggttatca ggtgcaccaa taagacctct agcaataaga tgtatatatg
     5521 aagcttctca agttgtaaat ataccaataa tcggtgttgg tggaatcact tgtggaaaag
     5581 acgtggctga aatgatgatg gctggagcaa gtgctgttca agtatgtaca gaagcaatcc
     5641 ttaaagggcc ttctatctat ggaaaaattg ctaaagaatt aaatgaattt ttagatgaga
     5701 atggatatgc tgatgttaat gaaataaaag gattaactca taagaaaatg gcagctagag
     5761 ggtttagaac tcattcagta gctccagtag tggatgataa caagtgtatc aaatgtaagc
     5821 aatgtgtaac aagctgtgta tacgaagctt tagaagttac agataagttg aacatagatg
     5881 cagacaagtg ttttggatgt ggattatgtg taactagatg tccaaaagga gctttaagca
     5941 tctgctacaa tcaagtagaa gctatgaatg agtaataatt atatagaatt ttggtaatta
     6001 acttagagta ataggtatta ggataataaa atatatcaga taaacaaaca cacattatgc
     6061 attaataata cagtctaaag ttaataattt tattatattg atttaactaa atattagttt
     6121 agctgaatat taatttaatt aggtattggt ttaatcttaa tatagattta ctgcgtataa
     6181 gtgaactaac tagattactt taagttaatt ttttcatata aaataaattg atacattaca
     6241 agtaaggtgg agtctatatg ggaattgttt taaaaggtgg gaaagtagta acaagtgaaa
     6301 aaacttactg tgcagatgta agaatagaag atgaaaaaat agtcgatata ggtcttgata
     6361 tatcaaaaga aaatgatgaa atagttgatg ttagtgattc ttatgttata ccaggaggta
     6421 tagatacaca cacacacttt gacttagata caggcgttac taagacagca gataactttg
     6481 agacaggaac aaaggctgct atagtaggag gaactactac tatattggat tttgctacac
     6541 aagctaaagg atgttctttg aaagacggat tagaagaatg gcatgaaaaa gctaaggata
     6601 aatcctattc agattatggc tttcatatgg ctataacaga ttggaatgac tcggtttgta
     6661 atgaaatgga ggatatggtg aaagagggag tatcttcatt taaactgtat atggcgtata
     6721 aaggaagcct tcaagttgat gatggtgtaa tctttgaagc attgagaaaa gcagaagaga
     6781 taggagggat tattggtttc cattgtgaaa atggggacat aatatgtgaa cttgtggata
     6841 aagcaaagtc ggaaaataat ttatctccaa aatatcatca attaactaga ccagctataa
     6901 tggaggcaga agctacatca agacttatga agatagctga agttgcaaac tctaaagtat
     6961 atgtagttca cttaagctgt aaagaatcct tagaggaggt tttaaaagct aagaaaagag
     7021 gtgtggatgt aatagttgaa acttgtcctc aataccttct attaaatgat gaattatatg
     7081 atttagatgg atttgaaagt gctaagtatg taatgtcacc acctttaaga aataaagaaa
     7141 ataatgaaat attatggaat gctattttag ataatcaaat agacactata ggaacagacc
     7201 attgttcttt taattataag ggacagaagg aacttgggat aaacgatttc agtaagatac
     7261 caaatggttc tccaggtgta gaacatagga tgagtctttt atacacatat ggagtctgta
     7321 aaaataaaat atctatcaac aagatggtag aagtaacatc aactaatcca gcaaagttgt
     7381 ttggaatgta tcctaataaa ggaactatag aaataggcag tgatgcagat atattagtct
     7441 taaatccaaa caaaaaatcc aaaatagaag caaaaaaaca attacaaaat gtagattata
     7501 caccttatga agggtatgaa gtggattgtc aaatagaaca tgtttattta agaggaaaag
     7561 aagtagttag agatggagaa ttaatttgta atactccaag tgggagatat ataaataggc
     7621 gtctaacaaa gggggctaga gaatggtaga atttatatta aatggagaga aagttgttgc
     7681 aaaagaaggc aaaaaacttt tagacttcct tagagaagat atgaatataa cttcagttaa
     7741 aaatggatgt tcagaaggtg cttgtggaac atgtatggtt ataatagatg gtaaagctac
     7801 taaagcatgt gtatttaaaa ctgataaatt agctgggaaa aacataacaa ctatagaggg
     7861 attatcagag agagaaagag atgtttttgc atattctttt gctagtcaag gagctgtaca
     7921 atgtggattt tgtattccag gtatggtaat tagttcaaaa gcacttatag acgttaataa
     7981 taatcctact gaagatgata taaagaaagc tttaaaaggc aatatatgta gatgtacagg
     8041 gtatgtaaaa ataattaaag ctgtcgattt agcggcaaag atttttagag aaaatataga
     8101 tgtgccaaaa gtaaactgta agggtttagt aggtgaaaat cttcctagag ttgatgcagt
     8161 tggcaaagca cttggatatt ctgaatatgt agatgatatg agaattgagg gtatgattta
     8221 tggttcagca gttagatcta agtatcctag agctttagtt aaaaaaatag atatagaaga
     8281 agcaaaagct tatccaggag ttgttgcagt tctaacagct aaagatattc caggaactat
     8341 aaaggtagga cacttaaaga aggattggga tactttaata ccagaaggtg aaataactag
     8401 atacttagga gatggaatag ttttagtagc agcagaaact agagaagcgt tagaagaggc
     8461 caaaaagcta gttaagatag attatgagga attaacacca ttaagtacta cagcagatgc
     8521 acttagagaa gatgctccaa agatacatga gacagggaat atacttgtta aggaacattt
     8581 agtaagagga aacgctaaag aaaagataga aaattcaaaa tatgttgtaa ctaaaagata
     8641 tactacacct gctacagaac acgcattttt agagcctgaa tgtgcagtag ctggtcctta
     8701 tgaagatgat ggagtaataa tctattcaac agaccaaggg gtgtttgcca ctcaacatga
     8761 atgtgcagat atgttaggat taccatatga gaaggtaaga gttataaaca aaatggtagg
     8821 aggagggttt ggtggaaaag aagacatgag tgttcaacac catgcagcct tacttgcata
     8881 ccatacaaag agatttgtaa aagtacagtt aacaagaaaa gaaagtatta ttgttcatcc
     8941 aaaacgacat gccacagaaa tggaatttac gacagcatgt gatgaaaatg gatacttaac
     9001 gggtatgaag gcaacgataa tatctgacac aggtgcatat gcctctcttg gaggaccagt
     9061 gctacaaaga ctgtgtactc atgcagcagg accatacaat tatcaagata ttgatataac
     9121 aggaacagca gtatatacta ataaccctcc tggaggagca tttagaggat ttggagttac
     9181 acagagttgt tttgcaactg aatgtaatct aaatttactt gctgaaatgg ttggaatatc
     9241 tccttgggag ataagatata aaaatgcaat aagacctggg caagttttac caaatggtca
     9301 atttgcagat gaaggaacag caatggcaga gacactagaa gctgtaaaag aagagtatga
     9361 aaagcatcca cgtgcaggta tagcttgtgc tatgaaaaat agtggggttg gagttggtat
     9421 acctgatgtt ggaagatgta gattggtgat aaaagatggc aaagttcaca taagaacaag
     9481 tgcttcttgt ataggtcaag ggcttggtac tgttatgact caagtagttt gtgaaactgc
     9541 taacctactt cctgaaaata tagttcatga agttccagat actcatttaa caccagactc
     9601 tggtaatact acggcttcaa gacagacagt atttacggga gaagctacta aggttgcatc
     9661 aaataagtta aaagaagctt tagactctgg caaaacttta cttgatttag agggagaaga
     9721 gttttatgga gaatatgaaa gtattacaga taaaatgggt tgtgacaaag aaaatccagt
     9781 aagtcatgtt gcttatggat atgcaacaca agttgtaatt ctggatgaaa atggaaaact
     9841 agaaaaagta gtagcagcac atgatgttgg aagagctata aatccaatca gtttagaagg
     9901 tcaaatagaa ggtggagtag ttatgggtct tggatatgct ctaaccgaag attacccagt
     9961 agtcaactct gttcctacag ctaagtttgg tacattaggt ttatttaaat ctacagatat
    10021 accagaaata caagctttaa tagttgaaaa gaacaaagat ttattggctt atggggcaaa
    10081 aggtgtagga gaaatttgta cagttcctac tgcaccagca gtacaaaatg cctattatgt
    10141 atttgataaa gaatttagaa ctgagctacc actacaaaat actccatata gtaaaaaaaa
    10201 gtaacaaaaa aaagaaatat taaataaaaa aatagatgaa attgtaaata aaaaaaaccc
    10261 ttttgcataa ggtatatttt aaatgataga atatacatat acaggagggt ttttctttta
    10321 taaaataagt ttaataaaat tgagaggtga gcagaatgaa ttttaataat agtctagaat
    10381 taactcagtc tcagaagcta ataatgacta cacaacttaa gcaatcatta tcaatattaa
    10441 atatgagtaa gttagagtta gaagaagaga taaaaagaga agcagagaat aacccattag
    10501 ttgaagtcga gaaaagtggg gagataaatt gggaagagta tataaaagac atggataggt
    10561 caagacgttt agatagaaca gagataagct ataatcctga taatgaagtt aatctagaaa
    10621 atttagttaa atattcgtca aatttatatg aagatttaaa atttcagata agcctgtata
    10681 aacttacaga caaagaattg gaagtctgtg agtatataat tgatagttta gatgaggatg
    10741 gatatttaag aactgaggaa aaagtaattg ttgatacctt aaagatagat gaagaacttt
    10801 ttgaaaaatg tttaattagt gttcagcagt tggaacctag tggagtaggg gcaagaagtc
    10861 tttcagaatg tctgatgata caaatggcta gtttaggaat atataatgaa attttagagg
    10921 aaatcgttac aaaagactta aatttaattg caaataataa atacaaagag ataagtaaaa
    10981 agtacaatat gtcattacaa aaatgtgttg agctaataaa cataattaag acattagacc
    11041 ccaaaccagg aagaacttgt tcagtagaga aaagtgtata cattcaacct gatgtaacag
    11101 tagaaaaaat tgatgatgaa tttattgtat atttaaatga gaaagattct tatcaaataa
    11161 gaatcaatag ttattataaa gacatattaa aaaattcaca gtctgatgaa agtgcaaaag
    11221 aatttataaa agaaagacta aattcagcaa caggacttat gaaaaatata gagagtagga
    11281 aaactacagt acttaagatt gcagaagaga tagtaaaagc tcaagatgaa tttttgagaa
    11341 aagggacaaa atatataaaa cctttgaaaa tgaaagacat agcagaaaaa ttagaatttc
    11401 atgaatctac tataagtaga ggtgtaaatg gtaaatatat gctaacacct tttggcgtat
    11461 atgaatttag gtactttttc agtagcgcca ttgagactga gaataatgaa atggcatcta
    11521 gtacaagcat aaaaaaaatt ataaaggaaa caataaaaga cgaaaataaa aagaaacctt
    11581 taagtgatga ccatatatct aagttactaa aagaaaaagg aatcaatgtt gcaagaagga
    11641 cagttgccaa atatcgtgaa gaattaggaa tcccatcttc aagtaagaga aaagaatact
    11701 aataagtaaa agacaaatat cagtatggta aggggaaaag gctaattaag agatgaatgt
    11761 agattgggct aaaaatttta taaagtaaaa taatagaata tggaaaagta ttaaaatgct
    11821 ataaggtatt aaaaaactgg ggaacattaa aacattaaga tagcaaagaa cataaaaaac
    11881 taaagaagat taaaacatta aagaacatta gagctttgag gaatattaga actttaaaga
    11941 atattaaaat ttataaaata taaaaatact aaaaagaatt gaaaaactgg ggaacattaa
    12001 aatattgtaa aatgctaaaa aactagaaaa cataattaaa taagtgctta taaagaatga
    12061 ttaaattaaa taagtgtttg taaagaataa ttagatatta gaaaaagaag aatacctaga
    12121 taaataaact attattaaat ttaataatca caaaaggaaa acacgtaggg tataaataaa
    12181 aatcttaact agataaaagt aaacttagtt gtagataaaa ataactagaa ataaaagtaa
    12241 ctaaaaataa aattatttat agtaataaat attagaatga aaatataaaa aaatatatat
    12301 aaaaagcctt atataaaaag aaaaaatact gtgatttgaa aatggtggag aaatatttta
    12361 taaaataaaa aaataaaaaa gtttttgaaa aactattgaa atctatacgc aaatcctata
    12421 aaataaaaat atcttgtggg acttaaataa acactaggga caaaaaaagt cccgaaagga
    12481 aagcaataag gagtatgtta tgaagaactt attagaaatt caaaagaaat tgattccaca
    12541 ggcaattgaa cttatggaaa gaagatactc tattttaaga caaatatctt taagtgaacc
    12601 aataggtagg agaacattat caaatgtatt agagattagt gaaagaataa tcaggtcaga
    12661 aactgagttt ttaaaagaac aaggacttat agacgtagct gtatctggga tgactatcac
    12721 taaagaaggt tctgatttgt tagataaact taaggacatc atgagtgaca tcatgggatt
    12781 gtctaagtta caagatagag tcagagaaaa gcttggaata aaaagggtaa tattagttcc
    12841 tggaagctgt gatgaaaatg gaagtttact aaaagatgta actagatatg gatgcgaata
    12901 tttcttaggc attttaaaag atggagatat cgtttcaata acaggaggaa gcactatgtt
    12961 ggagttttca aacgttatta aaactgataa gagatatcca aactctacaa tagttccagc
    13021 tagaggaagt atgggaactg atgtagagat gcaatctaac aatatagttg cttcaataag
    13081 taagaaatta cattctaatt gcaaactttt acatatacca gatgagttgg atgaaagtgc
    13141 aatgaaaact cttagccaag tgccagagat taagacaact ttggagtata ttaaaagaac
    13201 agacatttta gtttttagta taggtagagc agatgtcatg gcaaagagaa gaaaacttcc
    13261 agaagacaag gttgatgaga tattatctaa aaatgcagtt ggagaagcat ttggatatta
    13321 tttcaacaaa gatggagaaa tagtttacaa actgaacact gttggaattg atttagaaac
    13381 agttaaacaa gtaaaagaaa ctattgcaat ctttgcaggg actgaaaaag tagaagcctt
    13441 attagctata tcaaatatgc ataaaaacat ggttcttgta acagatgaag aaagtgctta
    13501 taagatattg tcattattat aaaagtcaag acctagcaca gagctagaaa acatacattt
    13561 actttatagg aggtagcgaa aatggttaaa gtagctataa atggatttgg aagaatagga
    13621 agattagcat taagaaaaat gatggagcaa caagataagt tcgaagttgt tgcaataaac
    13681 gacttaacag acgctaaaat gttagctcat ttatttaaat atgatacagc tcaaggaaga
    13741 tttaacggag aaatagaagt taaagaaggt gcttttgtag taaacggaaa agaaataaaa
    13801 gtaacagcag aaagaaatcc tgctgaccta ccatgggctg aattaggagt agatatagta
    13861 ttagaatgta caggattctt tacatctaaa gataaagctg aagctcatat acaagcaggg
    13921 gctaagaaag ttgttatatc tgcaccagct actggagatt taaaaacaat agtattcaac
    13981 acaaacagtg acatattaga tggttctgaa acagttatat caggagcttc ttgtactact
    14041 aactgtttag ctccaatggc taaagtatta aatgataaat acggtataga aaaaggatta
    14101 atgactacta tacatgcata cactaatgac caaaatactt tagatggtcc tcatccaaaa
    14161 ggtgatttaa gaagagctag agctgctgct ggaaacatag ttccaaacac tacaggagct
    14221 gcaaaagcaa taggcttagt aataccttca ttaaaaggaa aattagatgg ggcagcacaa
    14281 agagtaccag ttgtaactgg ttcaataact gaattagttt gtacattagg aaaaaatgtt
    14341 acagttgaag aaataaatgc agctatgaaa gaagcttcaa acgaatcttt cggatatact
    14401 gaagaaatgt tagtatcttc tgacataatc ggaataagct acggttcatt atttgacgct
    14461 actcaaacaa aagttatgga agtagatgga aaacaattag ttaaagttgt ttcttggtat
    14521 gataatgaaa tgtcttatac ttctcaatta ataagaactt taggatactt tgcacaatta
    14581 gcaaaatagt cagttttgag aaaatagaaa agtagattga caaagtccga gtttgtagtt
    14641 taatactacg gactcaggac tttttttaag aaaaaatata taaaatacac ctaaataatg
    14701 ttacatacta tatataaaaa tatgataata tgatatattt aatagggtaa aagtatgtac
    14761 tatattatac ttggcaataa aaaattactt tattaaaagg agagatattt atgtcaatgc
    14821 ttaacaaaaa aactatagaa gatattgatg tgtgcggaaa aaaagtatta gtaagatgtg
    14881 actttaatgt gccattacaa gatggtgtaa ttactgatga aaatagattg aacggagctt
    14941 taccaactat tcaatattta atttctaaag gggcaaaagt tatattatgc tctcacttag
    15001 gaaagccaaa aggagaagct aaaccagaat tatctttagc accagtagca aagagattat
    15061 ctgagatgtt aggaaaagaa gttgtatttg cagcagacga taatgttgta ggagaaaatg
    15121 caaagaaagc aactgaaaaa atggaaaacg gagatgtagt attattagaa aatactagat
    15181 atagaaaaga agaaactaaa aacgaagaga atttctctaa agaactagca tcacttgctg
    15241 aaatatttgt aaatgatgca tttggaactg ctcatagagc acattgctca acagttggtg
    15301 caggcgagtt tttacaagag agagtttgtg gatacttaat tcaaaaagaa ttaaaattct
    15361 taggagaagc tgttgcaaac cctgtaagac catttacagc tatacttgga ggagctaaag
    15421 tttctgataa attagctgtt ataaacgaat tattagaaaa agttgataat ctaataatag
    15481 gtggaggaat ggcttataca ttcttaaaag ctcagggtta tgaagtagga acttctttac
    15541 ttgaaataga taaagttgaa tatgctaaag aaatgatgga aaaagctaag aataagggtg
    15601 ttaacttatt attaccagta gacgttgtta tggcagacca ctttgcacca gatgctactc
    15661 caatagtaac agaagatgct aatgtaaaag aagattacat gggactagat atgggaccta
    15721 agactatagc aaactttgta aaaactataa aagaatcaaa aactgtagta tggaatgggc
    15781 caatgggagt atttgaattt gaaaactttg caaatggtac attatcagta gctagagcta
    15841 tggcagagtt gactgatgca actactgtaa ttggcggtgg agatagtgca gcagcagtta
    15901 atcaattagg atttggtgac aagatgactc acgtatcaac aggtggagga gcatcactag
    15961 aatttttaga aggtaaagaa ttaccaggta ttgcagcatt agataataaa taaactaata
    16021 tatagaggag attgtaaaat gagaaaacct ataattgcag gaaactggaa aatgcataaa
    16081 acaataaaag aagctttaga atttgtaaat gaagttaaag ataaagtaaa ttctgataaa
    16141 gtagaagctg ttatatgtgc accatttact ttattaaagg atttaaaaga agctactaag
    16201 ggtacaaata taaagatagg tgctcaaaat atgcactttg aagaaaaagg agcatttaca
    16261 ggagaagttt cacctcttat gttaaaagaa atagatatgg actatgttgt aataggacat
    16321 tcagaaagaa gacaatactt caatgaaact gatgaaactg taaacaagaa agttttaaaa
    16381 gcattagaag taggaataga cccaatatta tgtgttggag aaactttaga acaaagagaa
    16441 gctggaaaaa ctaaagatgt ttgtaaagtt caagttgaaa aagctttaga aaatgttctt
    16501 aaagatgatt tagcaaaagt agttgttgca tatgagccaa tctgggctat tggaactggt
    16561 aaaactgcaa ctgctgaaga tgctaatgac gttatatctt atataagaga agttataaaa
    16621 ggattatatg gagaattagc taatgaagtt agaatacaat acggtggaag tgttaaacct
    16681 tcaaatgtag ccgaaataat gggtcaaagt gatatagatg gagctttagt aggtggagct
    16741 agtttagcta gtaatgatta cctagacctt gttaatttct aaggagtgaa atgatgaaaa
    16801 aaccagttgc tttaattatt atggacgggt ttggatacaa taaggatgtt aagggaaatg
    16861 caattgctga gtccaaaact cctaatttag atagaataaa aaaagagtat ccaaacacat
    16921 tgataaatgc tagtggtctt gatgttggtc tacctgatgg tcaaatggga aattcagaag
    16981 ttggacacac taacataggt gcaggtagaa tagtatatca agatttaact agaataacaa
    17041 aatctataaa agatggtgac ctttttacta ataaagtttt atgtgaagca atggataatg
    17101 caaaagaaaa ctctcttcat gtaatgggac ttttgtctga tggaggagta cattcacata
    17161 tagaccatct taaggctata atcaaaatgg caaaagataa aggcgtacaa aaggtgtatg
    17221 ttcacgcatt tactgatggt agagatactg accctcaaag tgcattagaa tatgccaaag
    17281 aagtacaagt tagtatggat gaaattggag taggtgaatt tgctacagtt tctggtagat
    17341 actacgctat ggatagagat aagagatggg aaagagtaga acttgcatac aatgccatgg
    17401 ttagaggtat tggtgaaaaa gcaaattcta ttgaggaagc tattcaaaac tcttatgatg
    17461 atggaaaaaa tgatgagttt ataatgccaa cagttattat gaaagatgat aaaccagttg
    17521 gaagtataaa ggaaaatgat tcaattatat tcttcaactt tagaccagat agagctagac
    17581 aaataactag agctttagtt tgtgaagagt ttgatggatt taaaagagaa gatattaaaa
    17641 acttctttgt atgcttaaca gagtacgata taactataga gaatgtgcat atagcatttg
    17701 gacctcaatc tttagccaac actctaggtg agtatctagc taaaaatgga aaaactcaac
    17761 ttagagctgc tgaaactgaa aagtatgcac atgtaacttt cttcttcaat ggtggagtag
    17821 aagagccaaa taaaggtgaa gaaagactat tgataccttc tccaaaggtt gcaacttatg
    17881 atttaaaacc agaaatgtca gcatatgaat tgacagataa agctttagat aaactaggag
    17941 aagataagtt tgactttata gtattaaact ttgcaaatcc tgatatggta ggtcatacag
    18001 gaagtataga agcggctata aaggctgttg aaactgtaga tacttgtgta ggaaaattga
    18061 tagataaaat agtagagtta ggtggaagtg caataataac tgctgaccac ggaaatgctg
    18121 aatatatgtt agacccagaa acaggaaaaa ctgtaacggc tcactctatt aatccagtac
    18181 catttattgt agttggtcaa gaatatgaaa gtgcaaaatt attggatgga ggaagattat
    18241 ctgatatagc accgactgtt ttagatatga tgaagttaga aaagccagaa gaaatgactg
    18301 gtcattcact tatttcaaaa tagtcgattt tgataagtag agaataatat ataaactaca
    18361 atataaattt aaaaataatg cagtttaggt ttattttgta aagtttagac tttatgacat
    18421 aagatttaga ttttgtaagt tctggatatt gtaaggtgta gatgaagata tattttataa
    18481 aatttacaag atagacaatt tataaaataa aaaattacaa tatagataat aatgtaaata
    18541 atgatataaa taataacaag atagacaata a
//
LOCUS       DAELJO010000063        18540 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELJO010000063 DAELJO010000000
VERSION     DAELJO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.063"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            118..1335
                     /locus_tag="KPB88_001859"
     CDS             118..1335
                     /locus_tag="KPB88_001859"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG0054139.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1430..1810
                     /locus_tag="KPB88_001860"
     CDS             1430..1810
                     /locus_tag="KPB88_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0054140.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2094..2576
                     /locus_tag="KPB88_001861"
     CDS             2094..2576
                     /locus_tag="KPB88_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0054141.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2597..3412
                     /locus_tag="KPB88_001862"
     CDS             2597..3412
                     /locus_tag="KPB88_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0054142.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3465..5015
                     /locus_tag="KPB88_001863"
     CDS             3465..5015
                     /locus_tag="KPB88_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0054143.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5112..6464
                     /locus_tag="KPB88_001864"
     CDS             5112..6464
                     /locus_tag="KPB88_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0054144.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETMEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELK
                     "
     gene            6595..7353
                     /locus_tag="KPB88_001865"
     CDS             6595..7353
                     /locus_tag="KPB88_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054145.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7400..8119
                     /locus_tag="KPB88_001866"
     CDS             7400..8119
                     /locus_tag="KPB88_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0054146.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8243..9643)
                     /locus_tag="KPB88_001867"
     CDS             complement(8243..9643)
                     /locus_tag="KPB88_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG0054147.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9647..10555)
                     /locus_tag="KPB88_001868"
     CDS             complement(9647..10555)
                     /locus_tag="KPB88_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054148.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10816..11463
                     /locus_tag="KPB88_001869"
     CDS             10816..11463
                     /locus_tag="KPB88_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0054149.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11787..14426
                     /locus_tag="KPB88_001870"
     CDS             11787..14426
                     /locus_tag="KPB88_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0054150.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQFGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14484..15587)
                     /locus_tag="KPB88_001871"
     CDS             complement(14484..15587)
                     /locus_tag="KPB88_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG0054151.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15947..16768
                     /locus_tag="KPB88_001872"
     CDS             15947..16768
                     /locus_tag="KPB88_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0054152.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16847..17058
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17145..18461
                     /gene="argH"
                     /locus_tag="KPB88_001873"
     CDS             17145..18461
                     /gene="argH"
                     /locus_tag="KPB88_001873"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG0054153.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7078 a         2152 c         3290 g         6020 t
ORIGIN      
        1 ttttgtgatt aattaaaaaa ttataaaaat cgtttacatg ttgattttta aaaaaaaaag
       61 atatagaatt atatagaaag taataaacaa aaattggatt tacgggagtg gaatgaaatg
      121 atgaatgtta ccttaacaga tgtaaaagag gctagagaga ccattaagaa catagtaaaa
      181 agaactgact tacttgaaag tgttaaactt agcgaaaaaa caggagcaaa tgtgttctat
      241 aaatgtgaga atcttcaaaa aacaggttct tttaaattaa gaggagcttg taacaaaata
      301 gcgagtttaa cagatgaaga aaaggcaaat ggagttatag cttcaagtgc agggaatcat
      361 gctcaaggag ttgcattggg agcaaaaatg actggtataa aatctacaat agtaatgcca
      421 gcaactgcgc ctttagcaaa agttagtgca actaaaggtt atggagcaga agtagtttta
      481 aatggtgcgg tttatgatga tgcatatgca aaagctgttg aaatacaaaa agaaacagga
      541 gcaactttct tacatccatt caatgataag tatgttatag caggacaagg aactatatct
      601 cttgaaattt tcgaacaatt agacaataaa gttgatacta tactatgccc agttggtgga
      661 ggaggaatca tctctggtgt tgctgttgca gcaaaagcat taaatcctaa tgtaaaaata
      721 gtcggtgttc aaactgcaaa tataccttca atgaaagagt caataaaaaa tggaaaagta
      781 acaacagcat ttaatgatac tacaatagct gatggtatag ctgttaaaac tccaggagat
      841 ttaacttttg aaataataaa tgaattggtt gatgaaatag tagtggttga agagacagaa
      901 attgcagaat caatattatt tatgatggaa agtcaaaaaa tagtttcaga aggtgctggt
      961 gctgtatgta cagctgctat attaagtggt aagtatgtac cagcaaaaga tgaaaatgta
     1021 gtttgtataa tatctggtgg taatatagac ataaatacgt tatatagaat aataggtgtt
     1081 gctttagcta aagaaggaag aagatattca tttagtacaa ttatggaaga taagccagga
     1141 aactttgctg agttaactag aataataagt gagaatggtg gaaacatttt aagtgctaat
     1201 caaggtaaat tatcagcagg ggaagctcta ggaaaacaaa gtgcggaatt tatattagag
     1261 acaatagatt atgaccatat agctagaata aagaaagcta tagaagaaaa aggttttaaa
     1321 attatagaat tataataaaa agtgtatata atatacaaga aaaatagtat aatagtgtat
     1381 gaagatacat tttatgtgta ttagattaac gaattaggag gattttaaaa tgaaacatga
     1441 agtaatacat actaatgatg caccagcagc tttaggacca tattctcaag ctataaaagc
     1501 tggaaacttg ttatttgtat ctggacaagt tcctcttgta ccagagacta tggaagtagt
     1561 tgaaggagat gtacaagctc aaactgctca atctttaaaa aatcttaaag ctatattagc
     1621 tgaaagtgga gctgattttt caaatgtagt taagactaca gtatttataa aagatatgaa
     1681 tgaatttggg gctataaacg aagtttatgc tgaatatttt ggagaaaata aaccagcaag
     1741 agcttgtgta gaagttgcta gattaccaaa agacgttaaa gttgaaatag aagtaatagc
     1801 tgtattataa aaatacaata tttttaattt gaaaaggctg aaaaactata ttctagtgat
     1861 tcagcctttt tataaaaaaa taaatcacaa taaagaacat gataaatatt gtcgaattaa
     1921 aaaataaaat taaaataaat attgcaatat ttttgagaag gttttataat gagtatataa
     1981 tttgaaacgt gtaactatta aattaggcat gagttcttgg atatttttat cctgaggatt
     2041 catgcctttt ttgtattgaa attcaaaaat ctatttaata aggaggaagt actgtgttaa
     2101 attttttcaa gaaaaataaa agctataagt tacatgcagt agtgagtggt aacagcatta
     2161 atattgagaa agtaaacgat tctgtgtttt caaaaaagtt aatgggagat ggagttgcaa
     2221 ttattcctaa tagtaatgtt gttgtagccc cttgtaatgg taaagtaact gtgcttacag
     2281 aatcaaagca tgcctttggt atggtctcag atgagggtgt tgaaatatta gtccacatag
     2341 gaattgatac agttagttta cagggtgagg ggtttaaaaa tgaagtgtct caaggagata
     2401 ctgttaaaaa aggtagtcca ataataagtt ttgaaagaga aaaaataaat tctcagggaa
     2461 ttgattgtac aacaataata attgtattaa atcatagcga attttcagaa ataaattgca
     2521 tggtagaaaa tgaagttgtt gcagggcaag atactgttat tgaaattatg aaataataga
     2581 aagggattaa ttcaatatga gagtagtaaa aatctttaat aataatgcat tatctacagt
     2641 aactaatgat aggaaagaag ctattttact aggtcttggt ataggtttta ataaacgtcc
     2701 aggagataaa gttaatgaag ataaaataga aaagatttat tatgttcaag accacatgca
     2761 aacaaaattt ttagaattat taaaaaatgt tagtccagaa gtgatggatg cttcacaaca
     2821 aattataagt cttataccag gaaatgaggg aaaatttaat aataaaggga tattatcttt
     2881 agtggaacat attagttttg caatagatag aatgaaaaat aatgtttttt tacctaattt
     2941 aatgcttttg gatataaaga tgatgtattc aaaagagttt gaattaggag taaaagcttt
     3001 agaaattata tatagagtat gtcatatcca tttgcctgaa gatgaagcag gttacatagc
     3061 tttacatttt gtcaatttac aatcaaatga taatttggca tatgatactt taaaatttgt
     3121 aaaaggtagt attgatttga ttaaagaatg ttatggatta gaattagacg aatcgagttt
     3181 aagtacatta cgttttagaa ctcatcttaa atttcttgct caaagaattt ttaagaatga
     3241 aatttgtcag gatgataaaa tgattgaaat gtatgattat ttaattaaca atcatcctaa
     3301 aaataaagaa tatttagaaa agttaaatgt atatattgaa aaggagttta ggtataaatt
     3361 agataaacct gaaaaaattt atctactact acacctaaca aaaatcttat aagaatgaga
     3421 tgaattagta atagaaatat atataatttt agggggggat aagattgaaa gaaaaactac
     3481 ttggaggact ggaaacattt gcaaaggcaa tggttcagcc attaatgtat ttatcgagtg
     3541 caggaatatt aatggtggta ggtgtactac ttacaaatga tatgttatta ggagtacttc
     3601 cattttttaa atgggagcca attcaaatat ttggaagcct aatttataac tgtataatgg
     3661 ctattattaa taatcttgct gttatatttg ctataggtat tcctgctgca ctagcaaaaa
     3721 aagagaagca caaagcagcg ttaataggat ttatgagtta tataatatac cttacagctt
     3781 ccaacactat gttaacatca ctaggtcaac tagcaaaaga aagcaagatg cttggactga
     3841 tagggactgg acaagctact attttaggta ttcaagttgt agatacaagt gtatttggtg
     3901 gaattatttt agggtatata gctggatatg tatttaatcg tacaagcaac aaaacattta
     3961 aaggagcttt acagatatac tcgggagcaa attttagttt tatatgcatg ttctttgtat
     4021 ccattttaat tggagttgtg agtaacttta tatggccatt agctcaacaa ggaatagctt
     4081 ctcttgcaaa tgtcatcaaa gatacaggta catttggttt attcttatat ggttttttag
     4141 agagaatatt agtaccaact ggacttcatc atttaattta tacaccattt cagttttctg
     4201 atttaggagg agtgcttaaa gttggtgcag atacagtttc aggagcctat gctatagtta
     4261 tgacagaaat gaatttacca gttgagcgtt tctcagatag tatatactac atggcaactg
     4321 gatttacaaa gatgtttggt tatataggta taggagctgc ctttatttat actgcatata
     4381 agcaaaataa gaagaaggtt aaggcaacga ttattccact aataattacg gcagtattag
     4441 cctctgtaac agaaccatta gactttatgt ttgtattctg tgcaccactt ttattctttg
     4501 tacatgcagt tatagctggt ctttttatag ctttattaaa aatatttgat gttactgcat
     4561 tatgtggagg gaacttatta gtatcagtta taatgaatac tacagtaggc gtagaaaaaa
     4621 cacactggcc agtaatgatg atattaggac ttgcacaaat tattgtatat tttgtagtat
     4681 ttagtttttt aattaagaaa tttaattata aaactccagg tcgtgaagat gagagtacag
     4741 ctacaggcga agaacaatca aaacaactta atttagatgc agggatagaa aatataattg
     4801 atgggttagg cggaaaagaa aatattaata cagtagaaaa ttgcatcact agattacgtg
     4861 ttaatgtaaa agatgaaagt aagattaatg aagatataat taatttaacg ccaaatagcg
     4921 ggattgtaag gaaaggaaaa gatatacaaa taatttttgg acttcatgtg catgaggtaa
     4981 gacgtgcagt agaagatttt ttagaagctt attaaagata tatatttata tagcaagggt
     5041 aaataacagc tttaagtgct gtttactatt tattaaagat acatataatt tatagaggag
     5101 gaataattat tatgattatt actattgttg gtggaggaag cacatttact ccaggtattg
     5161 ttaagtcaat tgcattaaga gaaaaagagt tagagcttga ggaaattcgt ttatttgata
     5221 ttgataaaaa acgtcaagat aaagtagctg tagttgtcaa ctggatacta aaagaagatg
     5281 ttaaaacaga tgttaaacta actgtaacta attgtgaaaa agaagcgtat acagatgcta
     5341 catttatatt tgcacaaatg cgtgttggta aatatgaaat gcgtgagcag gatgaaaaaa
     5401 ttccattgaa atatggttgt gtaggtcaag aaacttgtgg ttgtggaggg atggcatacg
     5461 gactgcgtac aatacaacca atgataaagg ttattgatga tgtagagaaa tatgctaaaa
     5521 aagattattg gatattaaat tactctaatc ctgctgcaat cgtttctgaa gcttgtcgta
     5581 agttaagacc aaatgcaagg attataaata tctgtgatat gccaatagct attatagata
     5641 tgatagcggg gtcattaaat atcaatgacg ttcataatat acgctatgat tattttggtc
     5701 taaatcattt tggctggttt acttcaatag attataaaca tagagactta atggaagaga
     5761 ttaaggaata cataaaagaa aataaaatat tattaccaaa atcttatata gaaaacttaa
     5821 ataaaagtcg tagtacagga aatcgccatg ctgtaagtag ctggatacat gtttgggaat
     5881 ctgtttatac aatgttagaa agcttcccaa aatatctgcc aaacacttat ttaacatact
     5941 atcttttaag taaagaaaca atggaacatt caaacataaa tcgtactcgt gcaaatgagg
     6001 ttatggaaag ccgtgaaaaa gacttatttg aaggtgtgag acattactta gaaactggag
     6061 aaatttctga aaaagcattt tatgcaggtt cacatggaga ttggattagt gatttggcag
     6121 tttcaattaa aaatgataca cgtagtcgtt ttttagttat aactgaaaat cgtggagcta
     6181 taccaaacat gccatatgat gcaatggtag aaattccagc atatattggt aaagatggtc
     6241 cagaagttat tgctcgaggt ccaatacctc tattccaaca aggattaatg atgcaacaac
     6301 ttaacagtga aaaattgata gtagaagctt atatagaaaa tagttatgaa aaagctttac
     6361 aagcattcac attaaataaa actattccaa gtatgaatgt tgctaaagaa attttggatg
     6421 aaatgataga agttaataag gaatactggc cagaattaaa atagtaggat aaaagaaagg
     6481 gtttttattt aataattaga tatgttttca gagtctttta tcaaaatgta ttcacaaaca
     6541 tatggcaata tattaaaata aagagtgata tttttggaag aatggggtga tgcaatgaac
     6601 attataaata aaattttttg gcaagaagat aggattggca tgattacaaa taatttagag
     6661 gcagatacac atagccactg tatgttacaa ctgttcttag ggatagaaga cagcattgaa
     6721 ataacagtca atgaaaaatt agttaaatgt aattgtatca tagtagataa aaatatctct
     6781 cattcatttt cagcaagaaa aaaagtttat tactctgcta ttattgaacc tacatctata
     6841 tatgcagaac agcttacctc aaaaatgaat gattttggct actggatatg tgacaatgat
     6901 ggattagaga aattacgtca acaaggaact tttttgatag ataactctag taaagaacaa
     6961 tatttaagat ttatggaaat gcttaataac tatttgaata tacctataac tctcaaacat
     7021 tatgatgaca gaattactga attgctcaat ttattacgta cttgtaattg tgataatcat
     7081 actatttcaa gttttgcaga taaagtgtcc ctatcagcaa gtagattatc acatctgttt
     7141 aaagaacaaa tagggatacc tttaaaaagc tatatcttat ttcatcaaat ggagtgtgct
     7201 tttagagaat tactttctgg aaaaaatgtt accgaagctt ctatgactgc tggattcgat
     7261 actccatcac actttgcagg gacagttaag cgaatgatgg gaatgcctgt ttctttatct
     7321 ttgaaagata gcgagttttt gaaagtatat taaatattct cgttatataa tgaagttgta
     7381 ctaagaaagg ggctttgtta tgaataaaga atatttagaa gaaacaaaaa tgttaaatta
     7441 taatgaacca caacttaagc ttttggtatc atcaaagaat tggttggaat tagatgattt
     7501 tcataaaata aaatctattt atgagtttgt acagaatgat attttatttg gatataatgc
     7561 ttctgatatg ttaagcgcaa cacaggtatt aaatgatggt atgggacaat gcaatacaaa
     7621 agctactctt ttaatggcat tattacgtgc agtgaatatc ccttgtaggc ttcatgcttt
     7681 tgatgtaaca aaagattttc aacgaggagc tacatccaaa ttgatttcac tattagcacc
     7741 aaaatatatt cttcatactt gggtagaggt attttatcaa gataggtgga ttgctctgga
     7801 gggtgtcata actgataaaa aatatttaga agcaattcag aagaaatttt ttaatcatgg
     7861 tggaacgttt aaaaagtatg caatagctac aaatgatttg aaaaatacat ctattgattg
     7921 ggatggtaaa gatactttta tccaaaaaga agctatcgtg tatgattatg gtatttttcc
     7981 atcaccagac gtattttttt ctactcattc acaacatatg tcaaaattaa aaaatttcat
     8041 atatgtacat cttatcagaa aaataatgac aaaaaatgta tgtaaggcta gaaacaatta
     8101 tattgataaa aatgaatgag tgtttaagag gtaactatat aggaaatata tagaataatt
     8161 aaaactgtac tggttaaaat gaataaaaag gagttatcac agagcaaact taaattgttc
     8221 ataggataac tccttttctg tattaacttc taataatttg atgtcttttt ataaaacata
     8281 tagatacaat taaaccagta aatgcaagag tagctgctat tgaaaatgca acatctattc
     8341 catgaatgtt agacagcatg tttgaactca tatgagcttt cttagtagca cttgtcatta
     8401 tagtaataag tatagcagaa cctatagctc cagatatctg acggagtgtg ttattaattg
     8461 ctgtggcatg agatataagt tctctatcta atgttttaat tccccatgta gttaatggca
     8521 ttaaaaccat tgaaatacca atcatacgaa tacaatacat caatgacaca tgaattaaag
     8581 atgtatctct gcctaaaaaa gcgaaggata aagttcctaa aaacaaacat cctgttccta
     8641 atattgatag agcatgaggt ccatatttat ctaaatgacg tcctgcaatt ggattgagta
     8701 caaccatcaa tatagcacca ggtaacatta atgaacctga agataatgca gaaaatcctc
     8761 ttacagattg aacgtacaga ggaatcatta atgtagcaga catcattgag gcatatacaa
     8821 tcataattaa tattgtagaa ataagaaatg gtttattttt aaaaaccctc aattctaaaa
     8881 aaggttcttt agattttaat tgacgcaatg taaataaaat taatgacata attcctatta
     8941 atattggtaa atatgtggca atattagtcc acccataatt accttgatta gtaaatccaa
     9001 ttaataaacc accaaaacct agagatgata gtactattga aggtatatct aattttagtt
     9061 tttgagtttc cccaacattt cttaataaaa taaatgatag tataacatct aatatagcaa
     9121 taggggaaag aaacagaaat aaactatgcc aaccaaatga atctactaac catcctgata
     9181 aagttggacc tattgctgga gcaaatccta ctgtaatacc aactaaactc atagctgcac
     9241 cacgtttttc ctcagggcat aaatagagta taataacttg taaaagtggc attagagaac
     9301 cagaacccat tgcctgcaac acacgagcaa tcaacatagt tgaaaaatta ttagaaaaaa
     9361 gtgcaataat acatcctata gaaaagatag acattgatgt aataaataac tttctagtac
     9421 taaatcgatt tattaaataa gctgttgtgg gtacaataat acctgcaata agtagataaa
     9481 ttgttgttaa ccactgacca acatcagcag taattttaaa atctgacatt atattaggta
     9541 atgctgttga caatagtgtt tgactgagag aacttataaa agcaccagtc attacaattg
     9601 ctattattaa atttcctttt ttattgttac tttgggtttc cataatttat aacgtctcct
     9661 tttttaattg atttttaatg ctgttcaata aaagtacttt tggtcttaaa gtgtcttctt
     9721 tttctaaata tgcaatcatt gcatccataa ttaaagaatc atattgttga ttttttccct
     9781 cgacgtataa tttttttatt gcttctagta atttaggatt ttcaaggaga gaagtatcag
     9841 ttttgattag ttcttctata tcttgtatcg ttttaaaaaa tagcgattta ttatgaaggg
     9901 aaaagtaaac ttcatcttct tgtttatgta aaaacattga tttttcaatt aaaggattta
     9961 gattatccat attttctaca actaaatcta gaatttgtat agtaaatttg gcaataaagt
    10021 atacatttat ttctcttttt tgtacatgaa taatgtatat aaactgatga attagagcat
    10081 ccaataagaa aactaaatca aatataattt tttctatttt atcaccatag ttatcaaata
    10141 agcttaattt gtattcattc aataagttag ctcgtgattt tgtttgcaat ttacttgtag
    10201 ccttacgttg ctcttcagaa aaattttctc ttatataggt atttatggta gttctagaaa
    10261 aaatattttt ccatacagca attatctttc tgtttaactt ttctgatgct ggaatgtcat
    10321 tattttcatt tatggaatgt ataatttcta aaaacttttc atcataataa ttgattattt
    10381 cgcagataaa actttcctta gtttcaaaat gcttgtaaaa agtagcttta gacattttac
    10441 actcttttac aacatccgat attgaagtat tttgaaaacc cttttggtca aaaatttttt
    10501 gtgcaatagt tataatgagc ctttgttttt ctgtcacttt attatctcct ttcacgcaaa
    10561 aacgtttagt actatagagt acctacaagt tttaataaat atattataca tcatttggaa
    10621 aaaaaatcaa tatatagact gtaatatgaa atttttgaaa aatttgttgt tatttttagt
    10681 cgtataaaaa agaaacttaa gagtttctta agaattgttt aaagaagttg caataaatta
    10741 gtggtatgat tatagtatga aagagaatgt cttgaaattt ttctgtatca aattccaatt
    10801 atggaggtgt agtatatgaa aaagaaaatt tgtgtagcaa taatttttgc tctatttata
    10861 gtttttgtag aaatgtttat tgatagctct agcgaaggaa gtaaaattat gacagctgaa
    10921 ttaagtaaat acaggaaagg cgtaaatgta ttatcaataa acaatattat agtagcgaat
    10981 aaaaaatata gtttgcccaa agattattct cctcaggaaa gcagtgaagc gagagatgct
    11041 ttttataaaa tgaataaaga tgctcaaaag tcaggattaa atttaaaagc ttttagtaca
    11101 tatagaagtt atgaatatca agatagatta tttaagtcat atgtaaaaga acatggagaa
    11161 aaagaagcaa atagattttc agctaaacct ggagagagtg agcatcaaac tggacttgct
    11221 tttgatattg gaggagatga ccagtcttgt tgggcaaata aaaagttcaa caatacaaaa
    11281 gaagctaagt ggttgtatga aaatgcatat aaatatggtt ttatacttag atatccagaa
    11341 ggtaaagaga atataacagg atatatgtat gaatcttggc actatagata tgttggaact
    11401 gaacatagca aaaactttgc aatgaataat ttgactttag aagaatattt acatataaat
    11461 taagagatgg tttgttaaaa ttaggttaaa ataaaaaata acctagacta ttagctgtgt
    11521 aaagtgatat aatgtaaata caatttagat aactactcga aggggagtag cttacacgaa
    11581 agtggtaatt aatcgtcaag acagtagaaa tactcggtta attagcaagg gattccttga
    11641 tagcaagacc ttcaaaataa gcattaggta tgtttgtttt ggggtctttt tttattcaag
    11701 gaatttttat ttaaaaaaat ttaaatttca agtatacata agagccccca aacataaaat
    11761 aaatttattg aagaggaggg caacaaatgt cttacaaaag tacttatcaa gaagtaatta
    11821 aaaatttaga ctcaagttta tctggtttga gtaaagaaag agcagaagag ttgttagaaa
    11881 aaaatggagc taatgaatta aaagaagcag ataaggttcc tacttataag cttttcctag
    11941 aaagttttaa agacccatta gtaataattc tattgatagc tgctttagta caaatatttt
    12001 taggtgaaac agtggagtct ataataattt ttgcagttat tattataaac tctgttctgg
    12061 gtgttgtaca aacaaaaaaa gcagaaagtt cattagaaag tttgaaaaat ttatctgcac
    12121 caaacgcaaa ggtaataaga gacaataaaa aaatgacaat tccagcaaaa aatctagtag
    12181 ttggagatat tgtattctta gaagctggcg attacatacc agcagatgga aggcttatag
    12241 aagcacaatc tctcaaggtt gtagaaggga tgttaactgg agaatcagaa ccagttttaa
    12301 agcatacaga aaaaataagt gaagatgtaa gtttaggtga ccagaaaaat atggtattta
    12361 gtggtgctgt agttgtttat ggtagaggtt cttttgtagt gactggaaca ggtatggata
    12421 ctgagatggg taaaatagct gggcttttag aaagtgctga aaataaactt acaccattgc
    12481 aacaaaaaat agaggatttt agtaaaaaac ttggtgtagg tattgtaata cttgctcttt
    12541 taatatttat aattcaagta gcaagaaatt actttatggc tggaaatgga gaaatgacac
    12601 aaattatttt agattcattc atgttctctg tagcagtagc agtagcagct ataccagaag
    12661 ctctatcatc aatagtcact atagtattgg ctgttggaac aaatagtatg gcaaaaaaac
    12721 aagccataat aagaaaatta ccagcagtag aaactttagg ttcaacaagt gtaatatgta
    12781 ctgataaaac aggtactctt acacaaaata aaatgacagt agttgattat tatatgtatg
    12841 gaactaataa tgatgagtta gataaagaaa aagtaaacta ttcttatcat gctaaattat
    12901 tgactatggt ttctacattg tgtaatgact ctcatataac tagtgatggt aaagaagttg
    12961 gtgacccaac agaagtggct ttaataaatt attcaagtaa aaatgattta gattatgata
    13021 aattaagaag taagtatatc agaataaatg aaataccatt tgactcagac agaaagctta
    13081 tgtctactgt aaatagtata tatggagatt atataatgtt tacaaaaggt gctccagata
    13141 ttgtgtttag taggtgtaaa tatgctttga aaaatggttc aaaagtagat ataacagatg
    13201 agataataga agaatataaa cataaaaatg aagagttttc aaatagagca cttagagtat
    13261 tagcttttgc aataaaagat gtcccagaag aagattttat accttcaatt gatgacgaac
    13321 atgacatgac attggttgga ataatggcaa tgatagaccc accaagagaa gaagtaatgg
    13381 atgcagtaaa agaggcaaag agtgctggta taaaaactgt tatgataact ggtgaccata
    13441 aaacaacagc agcagcaatt gcaaaagaga ttggtataat ggaagaagga gacttggctc
    13501 ttactggaaa agagcttgat gctttgagtg aagaacaact atatgaaaaa ctagaaaata
    13561 taagtgtata tgccagagtc tcgccagaaa ataaaattcg tatagtaaaa gcatggcaac
    13621 ataagaataa tataacagca atgactggtg atggtgttaa tgatgcacct gcattaaaac
    13681 aagcagatat aggaatcggt atgggaagtg gtactgatgt agcaaaagat gcttctgcta
    13741 tggtattggt tgatgataac tttgcaagta tagtaaatgc tgttgaagtt ggtagaacag
    13801 tatacagtaa tataataaaa tcaattactt atctatttgc aggaaactta ggagctatag
    13861 tatccatagt atttgcagtg tttgcaggtt ggtcaaatcc atttacagct ttacaacttt
    13921 tgtttataaa cttagtaaat gactctttac cagcaattgc attagggttt gaaaagtcag
    13981 aaaaaaatat aatgaataaa cctcctagac acccggatga aagtatacta gcaggaggaa
    14041 cattgaaggc agttgctata agaggttgta taataggtgt agttacaata attgcacaat
    14101 atataggatt acaagtatct ccagaacttg gagtagcaat ggcattttca acattgatat
    14161 tagctagaat aatgcaaaca ttacctgcac gttctaataa tcaaacagta ataaaattag
    14221 gttttttcag caataaatac gttataggag cagttttagt ctgtcttggg ctttactctt
    14281 taacactaat accagtgcta agaggaatat tctctatacc aacaacgttt ggattgtacc
    14341 agtttgggat ttgcctaggg ttagcattaa tttcatcaat attaatggaa cttaacaaat
    14401 taataaatag aagtgaagta aaataatcaa aaaattaaag ggcatattga ttttaacaat
    14461 atgcctttta taaatagtct aatttattct atattatcga ttatttggaa tgcttttata
    14521 acttcaggta attctttttc ttcaggcatt ctaaatctta tgaaattttt atcaagtcca
    14581 gtaaaatcac ttccagatat aactcttatt tttaatttag caaactcttg ttgtaaatct
    14641 atgtccttgt ttttatgttc aactgtcata attgatacag tatcactagt ttcagctata
    14701 ttaaggtttt tccatggaat taataattga ttttttatat cttttgtttt ttcttttaat
    14761 tcttctataa attgaacatc atctaataaa tttgctgcaa tactacgaga gatttcagac
    14821 acttcgtaag gagtagaaat ctttttaata gggctaacaa gttgttctgg aagaactgca
    14881 tatcctgctc ttagtcctgc taaaccgaat ccttttgaaa atgtttttaa agcaattaca
    14941 ttatcataat tatttagaag cttgacagct gaattttctt ttggcatgta ttcaccataa
    15001 gcttcatcta ccataactgc tatatcatat ttagctgctt cctttactat attttcaatt
    15061 gatgataaag gtataatttg tcctgtagga ttgtttggat tgtctatgta aattacttta
    15121 tattctggat ttattttttc aataaattct ttttcattaa acttgaaatt atcctctttc
    15181 tttaaaagaa cataatcata agttgcacca tgcattttta tatctgtctc atattctgag
    15241 aactgaggtg aatatcctaa aactttatct cccttttcaa taaatagtct atttaaaagg
    15301 taaataacat gtatagaacc atctgccaaa caaattctgt cagtatctaa tgctataaaa
    15361 tctttccaat aatcgactat attatctttt aaagcaattg aatgaggata tcctcttatc
    15421 atatcaaatt ttaaagcatt aaaagcttct acagctttgt ttgagtaaga cacggtattt
    15481 ataccttcac cacagtctat ctcgaataca tcttctgaat gtccagattc tattgcataa
    15541 ctttttttca actgcatttc aatagctttg ttaattttta gtttcatact ttatcactcc
    15601 ttatttagtc aataataaat gttataaaag ttgctagatt taaaatatta tgaaaatata
    15661 taatagtaaa aatgaatata aatattataa tagctcactt tatttaatat gattatatat
    15721 catatattgt aaaatttcta gcattataag actataaatt gaaaatattc agaaaaatac
    15781 tacaagaaac gatttatttt gctcttaaaa taagttaaat gaatgttatt aattttgtaa
    15841 attgaaatag tttaagtata aagttatgat ataatctaat aagggtaatt ctttagatta
    15901 tattaaggaa atataggtat acaataaaaa gttatggagt tggattatgg gatataaatt
    15961 aattgtaaca gatatggatg gaactttatt aggcaataat cataaagtta cagatgaaaa
    16021 taaaactgca ctacaaaaag ttataaaaag cggtattaat gtaactttag caacaggaag
    16081 agccttcgat tcagctaaat gcaatgtaga ttttcttaaa gaggacatgc caataatagc
    16141 ttgtaatggt tcattaataa gagagcaaaa tggaaatata atatattcaa ataagattga
    16201 tacaagaaca tgtttaaaca ttttagatgt attagataaa tacgatattt actaccaatg
    16261 taatagtata gattctatgc tttcgaaaaa aatagaagga cgtgaagata gattaagtgt
    16321 atttttaggc tctgaaacag aagtaattgt aaaagatgat ttgagagaag aaatctttaa
    16381 aaaagatata ctaaaatttg taatcataga agaaaaaaat ccatctattt tggatgaaat
    16441 aagaaaagaa ttgagaaaag ttcaaggaat aaagataact tcttcatggc caaacaatat
    16501 tgaagtcatg aatgaaggtg ttgataaagg taatgcagtt aaaattttag ctgaaaaaat
    16561 gaatatagac agagaagata taattgcatt tggagacaat tacaatgaca tagaaatgat
    16621 aaagtttgct ggtcttgggg tagctatggg aaatgcagaa gaattgatta aacaagaagc
    16681 tgattatgtt acagatacaa atcaagacag tggggttgca aaggcaattt ataaattcct
    16741 agagttaaaa gaatcactta gctcttaaaa cagtatataa taggaaaaaa taggttgact
    16801 attttgtaga tagtaattat aatacaaata gataaaacat aaaaatttag attcagtgaa
    16861 aaagagagta gatatagaga aaatctcagc gaattggggg atggtgtgag cctaatgata
    16921 acactttata ttgaaaagaa ctttggagaa tactacttga aattcagtat ggtagtaggt
    16981 aatctcgcct taagagaata gagtgggtaa acattgttta ccaactagag tggtaacacg
    17041 gaatatttcg tctcttggtc tttttgacca agagattttt tagtttcatt gagttacaag
    17101 acttataatt aaaatacaaa aaattaaaat atgaggtgaa agttatgaaa ttatggggcg
    17161 gacgctttag aaaagcggaa aatcaactta tggaagaatt taataaatct tttggctatg
    17221 attgtgtact gtacaaaaaa gatatcgaag gaagtgtagc acatgttcat atgcaagtaa
    17281 aatgtggact tttaacagaa gaagaaggaa agtctataac agaaggttta aaagggatac
    17341 ttgaagatgt agaaaatgga aagttagtat tggatggaga gtatgaagat atacatagtt
    17401 ttactgagat aaatttaatt caaagaattg gagatgttgg caagaagctt catacagcac
    17461 gtagtagaaa tgaccaagtt gcagttgata tgagattgta tgctaaagaa aaagcaaatg
    17521 atttggtgga tttaatatct gaatttaaag ctactataaa agatgttgct gacaaaaacc
    17581 cagtgatgat gccaggctat actcatcttc aaagggcaca agtagtgaca tttaagcatc
    17641 acttaatggc atattatagt atgtttgata gagatgagaa aagaataaaa aatgctatag
    17701 aaatattaga tgaatcacct ttaggctgtg gagctttagc aggaactacg catgatatag
    17761 atagaagtat aacttgtgaa caattaggat ttaaaaaagt agtagataat tttatggatg
    17821 gagtaagtga tagagattat ttactagagc taatgagtga tttttcaatt ataatgatgc
    17881 acttaagtag attaagtgaa gagttgatac tttggagttc tcaagaattt ggatttgttg
    17941 agatagatga tttatataca actggaagta gtataatgcc tcagaagaaa aatccagatg
    18001 gtgcagagct tataagaggt aagactggta gagtatatgg aaacttattt ggtcttttta
    18061 cagtaatgaa aggaatacca ttagcatata ataaagacat gcaagaagat aaagaaggtt
    18121 ttttcgatag tgtacatact cttgaaatgt gtatacaaat aatggataga atgatagcca
    18181 cactaaaggt aaatgaggat aaaatgaaac aagcagtaaa aaatggtttt ttaaatgcaa
    18241 cagaagttgc ggattatttg gttaaaaata atgttgcttt tagagatgca catggtatag
    18301 taggaagtat cgttatatat tgtgaagata ataaaaaagc aatagaagat ttaacattag
    18361 aagaattaca aaagttttca gatgctttta aggaagatat ttatgatttt atcgactatg
    18421 agagtatatt aaataaggga ataaaaaaga atttaaaata atgtatttat ttttaaaagc
    18481 tgtaaatttg aatgttatca tttaaattta cagcttttta ttgtgtaaga ttttattata
//
LOCUS       DAELJO010000064        18278 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELJO010000064 DAELJO010000000
VERSION     DAELJO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.064"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            505..1260
                     /locus_tag="KPB88_001874"
     CDS             505..1260
                     /locus_tag="KPB88_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0054154.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(1403..1831)
                     /locus_tag="KPB88_001875"
     CDS             complement(1403..1831)
                     /locus_tag="KPB88_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0054155.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2252..2452)
                     /locus_tag="KPB88_001876"
     CDS             complement(2252..2452)
                     /locus_tag="KPB88_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0054156.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(2635..3804)
                     /locus_tag="KPB88_001877"
     CDS             complement(2635..3804)
                     /locus_tag="KPB88_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0054157.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            4040..4861
                     /locus_tag="KPB88_001878"
     CDS             4040..4861
                     /locus_tag="KPB88_001878"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG0054158.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(4937..6325)
                     /locus_tag="KPB88_001879"
     CDS             complement(4937..6325)
                     /locus_tag="KPB88_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG0054159.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKNEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(6460..7218)
                     /locus_tag="KPB88_001880"
     CDS             complement(6460..7218)
                     /locus_tag="KPB88_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0054160.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(7220..7936)
                     /locus_tag="KPB88_001881"
     CDS             complement(7220..7936)
                     /locus_tag="KPB88_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0054161.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(7938..8645)
                     /locus_tag="KPB88_001882"
     CDS             complement(7938..8645)
                     /locus_tag="KPB88_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0054162.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(8761..9297)
                     /locus_tag="KPB88_001883"
     CDS             complement(8761..9297)
                     /locus_tag="KPB88_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0054163.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(9399..10469)
                     /locus_tag="KPB88_001884"
     CDS             complement(9399..10469)
                     /locus_tag="KPB88_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0054164.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVSNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNNSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNSEKEYTIKINSNSLEL"
     gene            complement(10462..11520)
                     /locus_tag="KPB88_001885"
     CDS             complement(10462..11520)
                     /locus_tag="KPB88_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG0054165.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(11507..11839)
                     /locus_tag="KPB88_001886"
     CDS             complement(11507..11839)
                     /locus_tag="KPB88_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0054166.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            12038..13159
                     /locus_tag="KPB88_001887"
     CDS             12038..13159
                     /locus_tag="KPB88_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG0054167.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(13287..15185)
                     /locus_tag="KPB88_001888"
     CDS             complement(13287..15185)
                     /locus_tag="KPB88_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG0054168.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            15500..16276
                     /locus_tag="KPB88_001889"
     CDS             15500..16276
                     /locus_tag="KPB88_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0054169.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(16368..17120)
                     /gene="xth"
                     /locus_tag="KPB88_001890"
     CDS             complement(16368..17120)
                     /gene="xth"
                     /locus_tag="KPB88_001890"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG0054170.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(17281..18231)
                     /gene="galT"
                     /locus_tag="KPB88_001891"
     CDS             complement(17281..18231)
                     /gene="galT"
                     /locus_tag="KPB88_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG0054171.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT         6168 a         2975 c         2000 g         7135 t
ORIGIN      
        1 aagttcttag cactattatt ttggccaatt atgtggtata agtggatagt aatttcaaat
       61 ggtactcttg aaaatatgtt atttaccata tatgcaatcg tcgcaattgt tttcatcatt
      121 ttatattcgg tgtttatgat aaaatataaa gacattacac aaatagattt cttttacagg
      181 ataagtactc tacttgcttt tatatttaca ctttttagct ttttaatata cccaaagagt
      241 ttatttttct tgtatttaaa gattattttt actggaatat acctttatta ttcaatagta
      301 aaaactctta aatttaaaga tgacgaaggt gtagtgggta ttatgagctc acttttatta
      361 attgtaataa ctttgttcta ctaaatttaa ttctatttga cttaatatgg ctttaaatat
      421 tagacatcta cattattttc tgatattatt taagatggta gtcttatatt aataatgaaa
      481 tacactatga aggagttaac tattatggaa aaaaaagttt tagcaacagt tggagaaaaa
      541 gagataacta atattgatat tgaaaatgca ttaaaatcat tagaccctta tcaagctatg
      601 caatttaaaa ctgaagaagg aaaaaaacac ttattaaatg atttagtgaa tcaagaatta
      661 ttcttcttag atgctaaaga agaaaaatta gatgaagaag aaatatttaa attagaaatg
      721 aaaaagatag aagaaaatgt attaaagcaa tttgctataa ataaagtttt atctagtgtt
      781 aatgtaacag aagatgaaaa agtaaaattc tttgaagcta ataagagctc tttctctaaa
      841 ccagaaagtg caactgctaa acatatatta gtagatagtg atgaaaaagc taaagaaata
      901 cttgctcaaa taaaatcaga agaaatatct tttgaagatg cagctcttaa acattcttct
      961 tgcccatcta aagatatggg tggtgattta ggaacattcg gtagaggaca aatggttcct
     1021 gaatttgaag aagctgtgtt ctctatggct aaaggtgaag taagtgaacc agttaaaact
     1081 caatttggat accatataat aaaattagaa gaccttcaag aaagtacaga gtctacattt
     1141 gatgaagtta aggctgaagt tgaaaaaagt ttattatatc aaaaacaaaa tgaagtttat
     1201 ggaaataaga taaatgctct taatgctaag tacggaaacc ttgtaaaata caacgattaa
     1261 tcataaggtc atatataaaa aagctgtctt ttcacaatat ttattagaaa agacagcttt
     1321 ttatattaaa gtttaaatat tcagtttcat attatcattc ttttattgta aagaactcag
     1381 cttaaaagtt taaataagat ttttaattat cattagctaa atttttatat ccacgtatta
     1441 tctcataaca gtaacctatt acatctcttc tttttattat cccaataaat gtgtcttggt
     1501 catctataac aggaatgaaa ttttgattta aagattttat aactaaatct tcaatatctg
     1561 cattgatgga aacaggagaa ttatccattt ttctatttat atctgtcact ggaacatctt
     1621 ccaatccctt taagtccaga tttaaatcat tttttaatgt ccataaaaaa tcaccttctg
     1681 ttatagttcc aacatattta ccatccttac ttattattgg aattgcagag tatttgtgat
     1741 attccatttt ttctagggct tgccttatag tgtagtcttc atatatatat gctacttcac
     1801 tttttggtgt tagaaagaat aatatgttca ttacactacc caccgttact gaaaacttat
     1861 aaatattact caatatttat aatgttactt ttttgcagta actttctcct ttcttatttt
     1921 ctaagtattc ttaatactat acgctgtaac aagcgttttt cctttgctca ttttaatctt
     1981 attatgcttt attaatattt ttatatggtc tatcagtatc acttattctc tttttagaaa
     2041 atttactaaa aattaattga cttaaactta gccataataa ttttactcta cactattaat
     2101 tttaacatat ttatctcatt attaatgatt ttattttaat aattttaatt tattttgcta
     2161 ttaattttat ttacaaaaac tgaaaaaacc gctaaatgat tagcggctta tctctcatat
     2221 tactagaaca ctaatatata tgaattttta attataatct aactacgtta gcagcttgag
     2281 gacctttagc tccatctact acttcgaact caacgctttg tccttcttct aatgttttgt
     2341 atccatctcc agttatagct gagaaatgta cgaaaacatc tccttcacct tctacagata
     2401 tgaatccaaa tcctttttcg ttgttaaacc atttaactac accagttttc attaataaat
     2461 cctcctaaaa aaacacaaat atttagctac tcttttctgt aaccttatta ctactctatc
     2521 atatatagct atgtttgtca attattatta tttttataca ttaataatca atttttttac
     2581 tttataattt taaatgatta ttatctttta cactttattt ttctttataa ttttttaata
     2641 atttattttt attatatgga atcttatgga cttatcactc cagcctaaat cccatggcac
     2701 caacattcta tcagtattat ggcaagttac caaaggatat cctcttgagt ctaatcctgt
     2761 cactgtcgaa acatgtgtta ttctgccttt cttctcataa ccaacaaaat cccctggacg
     2821 taaattataa gcttctttgt atacttcttc ataggaacct ttagcaatta atgaacctct
     2881 tccactatac agtgcataat ttttaaaagc ttgtgcattt acccatgctt ttgtacctgc
     2941 tctctcgcta taattccaaa ttgagttctt tttgaacctt ccactttcaa acattatctg
     3001 tgatgcaaag tttgcacaat ctccaccttc tggattataa tccctatagt ttggattaaa
     3061 tttcatacca tgttcttcat cagctgcggc tccacagtat ttatgtgcat aatctatagc
     3121 ttttttttgt tcatctgtaa gctgtaactc tacaccatct tgttgaccaa tataatttct
     3181 tatatcatca gattttatat tttctaaatt taaagagtct gcaaaagggt ctgtatacca
     3241 ctctttagtt atgacatatt gattatctat aatttttaca tttagataat gatatgtacc
     3301 tattctaaag gcattttctt tgtccttttg attttcatat gaatacttat attcagtgga
     3361 tactgaacag attataccat agaggtcttt ttctttttcc tttatttttt taatcttaac
     3421 ccttgtcttt atgtctttaa attttacacc ttgtttacta gaccagtttt ctaaatattt
     3481 catctttttt acttcatttt cataagccca cagaccaaat tttttatccg tctcataaag
     3541 accttttaaa atgttttcat ttttctctaa tattgcctta tttctgtagt caaataagtt
     3601 ttcaagcaat agtttatatt ggtcttttac atcatcacct tcaacatttg cagattccat
     3661 gactttttct tccatatttt tcatacttaa atcattgatt aaaaatacac ctgctgtagt
     3721 agctaaagac aaaaatacaa atgccattat acctttgatg tacttattct tatttttcag
     3781 tattttcaaa gctacacctc ccaaatagta caccttacaa atcaaattac aaagaatatt
     3841 ctgtcacata tagtaatttt catatttaat aattctctat tttattatgt ccaatattaa
     3901 aaattaattg tatcaataca ctgttttttt tacaattttt tattttataa tctacttttt
     3961 tctttttttt atagtaatat atatgtatat aaatattttg gtatcattat aattaataca
     4021 tactaatgga gggtttttta tgcaaaaaaa agtcgcagca atcaatgact tatctggaat
     4081 aggaaaatgc tcattatcgg ttgctatacc tatactttca gcattaaagg ttcaatgttg
     4141 tccatttcca acggccatac tttcaagtca aactggttat cctgaattta ctttcttaga
     4201 ctttactgat gaaatggtaa aatattcaaa tgtttggaaa aatttaaaag tcaattttga
     4261 tagtatatat agtggttttt tgggttcaaa gcatcaaatt gagatagtgg ctaattttat
     4321 aaacgattat cccaatgctt ttatcgtagt tgaccctgtg atgggtgata atggagttat
     4381 gtatccaata ttcacagaag aaatgagaca ggaaattaaa gaattagtta aacattcaga
     4441 tttaactact cctaatttaa cagaagcttg tttcttaact ggaaatgatt atactaaaag
     4501 cgattataat agagatgagc ttatatatat tgcaaaatct gtatctgatt tgggacctag
     4561 caaagtcgtg attactggta tattagaaga tgataatata ttaaatttgg cttacgatag
     4621 agataatgac catgtattct ttacatcagt caaatataat aattgttctt acagtggaac
     4681 tggagatata ttcacatcta tactttgtgg tatgttagta aataaacatg atttgggagt
     4741 agcggttaat actgcaacag attttatata taaaactata aattacacat ctcaatttga
     4801 tactgataga aatgatggag ttatgtttga aaacttttta tcagatttaa ccaatatata
     4861 aaatcaaaaa aactatccat tttataaata cctataaatc ataaaattta ggtatttaca
     4921 aaatggatat tatttactaa actattggta tttcaattgt taccatagca ccaccagttt
     4981 tcacagaatt ttcaatatat aaaatcccat tatgtttttg tacaatatta tttacaatat
     5041 agagtcccat tccataatga gatttagaat ttctactcaa atctcctgta taaaactgtt
     5101 ctgtagcttt tgacaaatct gctttagaaa aaccacttcc acaatctgta actacaaatt
     5161 ttaagtattt attacgtact gatacactta tgtatagttt actctggctt ggtgaataat
     5221 ctatagcatt tgaaattata ttcataatag ctctgaataa cagttctcta tctatagtta
     5281 tatttttagg caaattatct tgtttataaa actccacatc taagttttta gtactagcta
     5341 atgcttctat ttgcactaag acatctttta taaactcact catactgatt gcttctttat
     5401 ttaatatata gccattctct gtttttgata tatctattaa agtattaata tatttttcta
     5461 tttgagtgac accctttgag atatattcca tgtattctaa taactcagga ttttcattta
     5521 tttcaattaa taagtcagta tttccatgta taattgttag tggtgttttt atatcatgtg
     5581 ccagtgcaga tatctgttcc tttctatttt cttctagcat ccattgtttt tctaaagaat
     5641 cttttaacgc caacttcatt ttatccatag aaaaaaggac attattaatt tcataaattt
     5701 tactagattc tacaacaaaa tctaaatcct gttgttctat tttttctgta gtatttttta
     5761 aaagctccat ttcaatatta aatttcttgc caaatacttt tgataatata acaatttcaa
     5821 taaaaaatat tatactaaaa ataatcatac ccaatgtctc tggttttggt aaatatgctc
     5881 ttaaaattgg agaactatac tctgatacta ttttgtattt tattataaaa acttcatttt
     5941 tcttaaaaaa ctttatataa ctttctgaat atgcatgcct ttcttcaata tctctcatta
     6001 aattccatac ttcttttgat tcttttttat taaaggtacc tgaaattaaa ttcccatcta
     6061 aagtataaac cccatactca tatatacttg gaattatatc ttcagttact ttttcacttg
     6121 aaataatctg ttctttatat ttattaaatt gtttttctgc ataattagct ggtaaaatta
     6181 cacctgatga taataataca gaaaatgata acattaaaag gagtacaatt gaaattgtca
     6241 taataaagaa taaagataaa tattttataa aaatagttct taacttttca acttttttgt
     6301 tgttagattt tttacctcca agcatttgta ccctgtcctc caatagttta attaattcat
     6361 aattaatacc tagccagttt tttatttgat tattttatat ctttttattg ctaagttata
     6421 ttttaacata ataatataaa taaacagtca atttttactc tattcttctg attttttacc
     6481 ttcccattta gaaaaccaaa tacataaaat tacaaatgct aaaattgtta caaaaccaca
     6541 cattaaacct gttgattgat atgcttttat tgattcaacc tttatatatt caaccgtttt
     6601 agaacttgaa aaagacgacc aaatagttat aaatctaaca ccccatgcac aaggaaaata
     6661 ctgccaaata ccagacccca atccagttaa taataatgct gaaagtaaca tttcaactat
     6721 acctactcca attgatgttc cttttccgaa ccttaaactt aagaataaat gtaatatgta
     6781 ttcaaataaa ttacacccaa ttaaaattag aattgaaatc atataaaagc ttgatgcaaa
     6841 gtaagcctta gatataccaa atttaaaacc taatacagac actaatgttg ctacaaaacc
     6901 aagacatact aagattatat atttacttat aagagtaaga tatttgggat tagatgttgt
     6961 taacatatgt ttataattcc ctgcataata ttcttgttca acaaacattg aacacacaac
     7021 acctgacaat aaaggaaaag ctattgctat aacttgtaaa tatgctacta tctttagtat
     7081 attatcagct cctgaaacct tataatacca taaaaacaat cccatcccca ttaaagcaat
     7141 gcaaatgtgc attagtatta taaaatttct ctttaatttt attaaatcag aacaaaatat
     7201 tcttattaac tgtctcatat tacttcacct ctctattatt gaaccaaacc cttgtagcta
     7261 aagcaaataa tacaaataaa acaatagata ttaatactcc tggcaaaata acagcattat
     7321 ttaaaaattc acttccctct ggtacaggaa gaccatttgg cagaatatgc aatacaacag
     7381 ccattaatct tgaaggaatc gaatatggaa acatatacca catttctgta gttgagaaaa
     7441 cagcaactcc aacaatagta aagaacatat ttgttagaac tgtaacaaat ataccaaatt
     7501 ttgaagctaa aaacatacaa agaggaatct gccacataaa tagtaaaata attaatatag
     7561 ttgcaatgat attttgacct attgaaatag tttctttata gattatccca acaaaaacta
     7621 cacctattgc agaaataatt gatgctataa aacaacaaat aatacagtaa atcatttttc
     7681 cagtccaaac gtctttcatt tcaattggca atggtaaaat accacgatat ttactttttt
     7741 catcttttaa tactattaaa gaacatatta atgttaatgc tcctggaaga aatgtcgtat
     7801 accaccagtt gaaagcagca ttttgccctc cacctagtcc acacaaaagt atacaaaaca
     7861 ctggaactat ccatatcaac ttcattccaa aagttccttt catcttttga aattccactc
     7921 taataaatct ttccatatta ctgccctctc tttctatttt cagctacaac tttcataaaa
     7981 agttcttcta agttttgttc ttttgatact tctccttgat aacccattac tccattagaa
     8041 attattccta tttcatctac aacttgttcc acttcagaca agatatggct tgataaaata
     8101 actgtaattc cttgttttgg aaaagaacga atcaattctc taagttcttc aataccaata
     8161 gggtcaagac catttgtagg ctcatctaag attaaaagtt ttgggtgatt taataatgca
     8221 atagcaatac caaggcgttg cttcatgcct agagagaatt gacctgaacg ctttttaaag
     8281 gtatctctta aatctacagt ctctaaaact tcttcaatgc gagagtcagg aagaccaagt
     8341 aaagtagtat ggacttttaa attttctcgt gctgttaaat tctcatatat agcaggagac
     8401 tcaataagag aaccaatatt tgataaatcc tttctagtcc atctatgtcc ctcaaacatt
     8461 atttttcctg aagttggatt taaaatccca gttatcattt ttaataaagt agatttccca
     8521 gctccatttg gtcctaataa tccatatata gaattagatt taattgctag tgaaatgtta
     8581 tcaactgcca tctgctcctt aaatttttta gaaactcctt ttacctgcaa aatataatta
     8641 ctcataattt tcatcctttc ttttgtcaaa cttatttaca agaatcattc taaatcataa
     8701 atataaggat tttataagaa ttaaaaaaca aaaaaagatg tgttaaaaac acatcttttt
     8761 ttatcttaat tctattaatt ttccattgta gtatatctct acttcttcaa tccaaatgct
     8821 attatcttct tcttggatga catttttaat aatattattt atagtatata ttatattttg
     8881 ttcattcaaa taatcatctt tccaagaaac tctacttttt ttatctggtt ctatcaagtt
     8941 tattttaaca agattatctt tttccttaac ttctacttcc atgtctaaac cattaaaata
     9001 ctcatttgat aatgtatcta agattaatcg cagtttatcc ttaagtgaac tttcttcatc
     9061 aatctgtaag tactttacta ttttctcatc ctttgaaatt atctctatag gtattttagt
     9121 agttttttta gaattaatca ttttactagt tgtttcttct tttgcatctt catccttctt
     9181 tacaactgct gattctttag aatcaactag ctcaactgca ctgatgctat aatccataca
     9241 tgccgtactc aataaagtag tagcaattat taaaaaactt actgctattt tattcataag
     9301 tatccctctc ctaattctct ttcgatactt attataccat agtatagatt atatttttat
     9361 acttttaaat caatttataa attattatag taaattattt acaattctaa gctatttgaa
     9421 tttattttta tagtatattc tttctctgaa ttcgacagat ttttattcaa aagccctttt
     9481 atttccctcg tatttctata cttgttgcta tgttcttcac tatgttcata atcatatgaa
     9541 ttattgtcat attcttcatc attattaaat tcttcaattg cttcttttga ctttatatca
     9601 caattaaatt tatacttatt tactggtata tcaagtctta ccttagaatt tgaaacttct
     9661 atgtccagga acttagcaat tggcatatta gagtacaagt aaaattcact ttcataattt
     9721 gaatttctac ctattttctc ttttatattt acattttcag gtatatactc ttctatatta
     9781 ttgataaata catcttcatt ctctgaagat atatacaatg actctgattc tatgtttaca
     9841 tttttaacgc ctaaaattga ttttgttgac aagtgaagat tgttattaga tattaagttt
     9901 aggttttcta atctatttac tttgttctct gttatagctc tactaaaacc tccataagaa
     9961 gttttatatg ttatagtatt tgaagctaca ttatcgtata caaataagtg tgagcttata
    10021 cttgatgcat ttatgtctac attatttggt acatatatta taatttctct ataatcatgt
    10081 gaaaacatac tatttataga tgtatttaat aattggtcga aatttttaat ctttttaatt
    10141 ttattttcat attttctttc accttttaca actaaagtct tatcttttaa agttacattg
    10201 tatttgtaaa actcattatt tcctcttgtt gttacacgca catcttcact tgaacttttc
    10261 tttattgtaa cgtttatatt gtcaacagct atatctaact tgtcaatatt tactttttct
    10321 tgataaagtt tattttcttt tttaaattca gcttcctttt ctaaaccaaa gtttattagg
    10381 cttggcataa ctaaaatccc tgaccatgtt gtccctatta tacctattac caataaaact
    10441 gcagcaaata tagcaagttt tttattcatg tttatcttct cctttcttat tttctatatc
    10501 ttcaaggtcc ttatatatcg cctcaaaatt tatactatca tcattatatt catcagtttc
    10561 ttttatgctt tcttcttctc tattgtagta atcattatta tatgaatctt cattaatgtc
    10621 tttattcaaa tcttcatcat ttaaagtagc tttttttctt ttttccatct tttcatttgc
    10681 ctttatataa atattttttc tctttatcca atgtatataa ttatttagta attgtttacc
    10741 tagttttatg gcaaataata aaatttgcca tgcaagtatc tcaagcccta caaatgctat
    10801 ggttgcgaaa atacaaaaca tcataacatc tggaaccatt gctataaatt taatcacaaa
    10861 tggaattaat ataacaaaag caattaatga gcatactaat gcaatagtag cagatacaat
    10921 agcacataca aacgcaaatg ctggaacaat tagaaataaa gataacacta ctaataaccc
    10981 ctttataact tgtctggata atcctcctac accttcttct atacttggtg taagtttatc
    11041 atttatattt tcttttgatt tattgtactt ttccttagct tcatctttca agatggagta
    11101 atcctttttt acatttgatt taaatctact aaatttacca tttaatatgt ctgtattcat
    11161 tatttttcca tctatatttt cttttgattt actatacttt tcttttgctt catcttttaa
    11221 gatagagtaa ccttttttca tatctgattt aaatttacta aacttatcat tcaaaacatc
    11281 tgtatttatt attttttcct cttcaatatt tttttcttta gtttgtaaga taagttcttt
    11341 tgctatagat tttggtgagc caagtgatgc tattatctcc atatcactct ttccctctat
    11401 aagaccatct acgaaatact cttcataatc tctcaatata tcattaactt cgctctcaga
    11461 aaaatctttt tttagataat cctttaaaat ttcaagaaac tcagttttat tcaagttcat
    11521 tacctccctc atcttcactt attaaaatct caacagcctt tacaaattct ctccactctt
    11581 ccatcagatt ggataaattc tcttttccta gtgaagttat tctatagtat tttctagcag
    11641 gaccttcatt agattctagt atatatgttt cgaaatacaa atcttttgta agtcttctta
    11701 gaataggata tattgttcct tcatttacat ctattacttt agagatgtta tgtactatct
    11761 catatccata catatctttc ttagatatga gagcaagtac acaaatttct aaaacaccct
    11821 ttctaaattg tgtgttcata tactccatcc tttctttaaa tacacagtat tgtgttttac
    11881 aaggtactat gttaaaactt cttatatcct cataataaca cagtactatg ttctgcacaa
    11941 tagttatttt taaatattct tgttgattaa atccttctat aaatgtataa tactctaatc
    12001 agatatattt tatgaaagtg gtgataaatt ggctaaaatg aaagatgata aaacaatatt
    12061 aatcctaact gctcaatttg gtgctggaca tataagtgct gcaaaagcag tcaaagaatg
    12121 tattattgaa aaatatagta actataatgt tgttatacaa aactttataa atgcaagtat
    12181 tcccatgatg aataagccta tggtaaaact ttatgaaaac aatacaaaat atactcctgg
    12241 attatataat tactattatt actttaagaa atcgtttgat tcaagacatg atttatctca
    12301 taaattatat acacctaaac tttctgagta tattgctgat ataaatccag atttgattat
    12361 ctctacattt ccactagctg ctgcttgtgt aaataacttt aagataaaaa atcctgatat
    12421 aaatatacct acacttacag ttataacaga tgttgtagat agtatggaat gggtttttga
    12481 aaatacagat ttgtattttg ttccttctcc tgaaataaaa aatagatttt ttcagaaagg
    12541 aataaatcca gactccataa aggttactgg agttcctgtt gataagagat ttcaaattga
    12601 aagtaaagaa ctatgttgtg ataaatatag actattactt ctaggtggag gaagaggtct
    12661 atttgatata gatgaggatt ttatgcattg gatagatgaa tttattgaag aacatagtaa
    12721 ttctatagaa attacaatag ttactggtaa aaacaaaaaa ttatatgaca atctaactca
    12781 taaaaaacct ctaaaaaata ttaaagtact tggatttgta aacgacatgt ataatctaat
    12841 aagagaatgt gacctaatgc taactaagcc tggaggagca actatatttg aagctattca
    12901 atcacaaact ccagtactcg taaaaatgcc aaaagttgga caagaaattg aaaacgctaa
    12961 atttataata gataagggtc ttggcatgat ttatagtgat gacctagacc ttaaaaacat
    13021 attttataaa ttagtatcaa atgaatttga ttctataata aactttatga aaaaaaattt
    13081 agaagaattt aaaacagtta ttcatccaga gaaaattgct gattatatat cagaattaat
    13141 agataatcat tacagttaaa ttgtactatt aaaaaatttc aaacataaaa ttaaaggcaa
    13201 tcaagtatgc ttttacactt gagaatttac tcatagtgtt taataatact tgattgcctt
    13261 tatcatatca aaatcaaatt gttatattag tatatatttt tcttgtactg catatacagt
    13321 tgttgtattt cttccatact tctctttaat tctatttgta aatctgaagc agtctggtaa
    13381 tccttatttt ctatagcttc tctcactcta gtgaatagtg attctctagt taaagtatcc
    13441 ttcataatat tgaactttaa atccgatatt gagttccaaa ctacctcatc aagcgcatcc
    13501 atattttctt ttgtgtgtat cttgacaata cttcttattt tttgaatccc agcctctatt
    13561 attctagttt ttaatttctt ttgccactta tctatagctc caatcttaaa tgattctatt
    13621 atactatcag taaatacatc accttgtttt aaagatttta attttgaagc atatatttct
    13681 aagtttttca tattttcata aactgtagct ggtggtattc caaatcttgc atttctttct
    13741 tccaaactat aatgttcaaa tacatcattt tcatctctat atgctctatc tttttctaaa
    13801 tagaaacttt cttcacctac atttttagat aactcttttt caagttcttt tgtactaaga
    13861 ccactcttag ctgctgcttt tataccatcc aacattgttt gataacatcc tgctattact
    13921 aaatacgtgt tagatagagg gcttggtgac cttaactcaa atcttacagt ctttggattt
    13981 ttcatatctc ttattaatcc aacaagcact gacctatttc ttgatggaat ctcataacta
    14041 tgccctaaag atgtaactat gcatacagga gcttcaaatc caggaactaa tctattaaag
    14101 ccatcattag aagctgttac aattgggttt aatacttcat aattgtaaag aagtcccata
    14161 agagccccat atcctagttc acttagataa tcttctttta aatcttttgg agcaaataga
    14221 ttttttatac ttccatcctt taacttcgca cttactccta catgtgtatg tccaccactt
    14281 cctgctactc catgaattgg ttttgcttta aatgtaactt ctagtccatg tgatgtaaat
    14341 acatcctcta taacatctct tactaataat tcattgtctg ctgcttgtaa cggtgtagaa
    14401 aacttccaag aaacctctaa ttgctccata gcatggtttg ttcttccatc tatactaata
    14461 gaactttgta ttcctccgac ctctttgtga gccatttcag ggtttacacc tagcttttga
    14521 agtataatta aacttttctc taaacaagtt cttataatac catgagttct tttccagtat
    14581 tgctctttta ggctttgtga tacatataat ttctctaagt cagctttatc ctcaggagta
    14641 ttaacccaga actcaagttc tgtagctgct gttaacatta tttcctctat atcatctact
    14701 gaatctattc ctatgttatt tattacatgt ggatattctc taaatatttc atacattgac
    14761 tttttaaagt acttatctgc tttttgaagt actcctctag aacatacttt tttattatca
    14821 tgtattaaaa atgcaggtat ctttaatgtt ccaacaggta gacctacttc ttcgtctata
    14881 tattccatat tgtaatctac ataccaatga caacttttat ctggcattaa atctactttc
    14941 gcattattta atgtagctat attataaagc tcaacacttg aaccatcagt ctgtatagct
    15001 gattccaaaa atttatctat atcctccaaa aataattcta caggaatctt ttcatctgtt
    15061 gcatttcctc ctaaatcaac ccccattaag gaaacaaatc ttatattttt attgcttttt
    15121 aggatttctc ttaattcttc attagtgtgt ttatctttct ctattacgta taatagacta
    15181 tccatttttc cactctccca taatttttat gtatatttta ttttgttaaa tacaagataa
    15241 atttcatttt ccttacattc atgaattttt cttgcaaatt attcgtgttt tattcgtgtt
    15301 tttatttata ttattatata tcaagtataa aaagttcgca atatttttta ttggaaaaaa
    15361 tattatacat acaatagttt aacattttta caaatatggg gtatactaaa ataaaagtat
    15421 ttttatttga aaatttaatt ttatttataa tataatctat agtaaaagaa ataataaaaa
    15481 aatttaggag ataaacttta tggaatacat aattttcgat ttggaattta accaaggatt
    15541 tgataaaaaa ctcaataaaa ctgtatccaa tgaaaagtgt ccttttgaaa ttatacaaat
    15601 aggagcaatt aaattggatt ctaaattcaa tataatagat acatttaata gctatgtaaa
    15661 acctacaatt tataaagaca tacatccctt tattagtcgt atgactaata ttaaaaattc
    15721 tgattttaat gattcgccta ctttccctga ggtctacaac aattttataa aatttatatc
    15781 atcacaagac cctatattat gtgtttgggg agctggagat ttaaaggaat tatacaggaa
    15841 tatcaactat cacaagctac catcaaattc cttacctaaa tcttatatta atatacaaca
    15901 acatgcatct aaatatttca ataatccagc aggaaaaagc attggcctac aaaatgctat
    15961 ttctatattg gaacttgatg aaaaaatgtc ttatcacaat gctttaaatg atgcatatta
    16021 tactgctaaa gtttttatta agatatacaa tccaagtata gtcccagata tatatcttta
    16081 tacgagtata aaacctaaaa ctataagata ttctaataaa aaaagagttg attatgataa
    16141 gttatttgat gagtttagga aaattttaaa tcgtgaatta actaaagatg agaaaaaaat
    16201 cattaactta gcttataata tggggaaaac aaatcaattt acattagaaa atgttaagca
    16261 aagaaaaaac aaatagagtt tatttagata aaaaataggc atgtagatta aaaaactagt
    16321 taactttaaa tttatatcta cagcccaatt tttatcataa ttaattttta ttttatctct
    16381 aattcaactg gacaatggtc tgaacctaat atttcagtgt gtatatccgc acttactaat
    16441 ctatcttcta attttttgga tgcacaaaaa taatctattc tccaccctgc attatttttt
    16501 cttgcattaa atctatatga ccaccaacta tagacacctt ctttatcagg attaaaatat
    16561 ctatatgtgt ctataaatcc actgtctaat aatttagaaa acttctctct ttcttcttta
    16621 gtaaaacctg catttttcat attattttta ggatttttta aatctatttc tttatgtgca
    16681 acgttcatat ctccacacac aattacaggc ttatgattat ctagtttggt taaataatct
    16741 ataaagtcgt cttcccatct agttctatat tctagtctct ttaattcaga ttgagaattt
    16801 ggcgtatata ctgtaacaaa gtaaaaatct tcaaactcta gagttatcac tctaccctct
    16861 ttgtcatgtt cttctatatt tattccatac attactttta aaggctcttt ttttgtaaat
    16921 atagcagtac ctgaataacc ctttctctca gcataattcc aatattgaaa atatcctggt
    16981 aaatctaact ctatttgccc ttcttgcaat ttagtttctt gtaaacaaaa tatatctgca
    17041 tctacttctt tgaaaaagtc taaaaatcct ttcccaacac aagctcttat tccattaaca
    17101 ttccatgata taaatttcat acttatactc ctcatctaaa cattgacaca ctccccatag
    17161 ctaaagatgg gggcttttca acaattttat tgtaaattct taatttactt ttattttaac
    17221 aatactatac tataatttca tctaaaatta ctatttactt aatacctaaa aatatatgtt
    17281 ttaatcaatt tttaactttt ttgttaaatc ttcagggctc attgaagaaa catacatatt
    17341 tgttccaagt tcaaatcctc caaaattata ttttcttggg attatgtgca tatgaacatt
    17401 aaaatacgat tttgtttcaa tctttgttgg atgagtgtga atacataagt taaatggcat
    17461 atatccattg atattgtata acttcttaaa caacttttca aatattgtag aaagctcctg
    17521 tatattatta tcatttaact caccaaactc tattttatct ttaaatatta ttcttacttc
    17581 tccactatac ttagttgcat atggtataag taccaaaaaa gcttctccat tataaacaac
    17641 tcttctatct aaacttattt cattttctat taaatcatca tacaaagagc ttttattgct
    17701 gtcataatac tcctttgaaa ccaacaattc attcgtaatt tcaggtggaa taaaagacat
    17761 agacaatatt tgagcatgtg gatgcatcaa agatgctcct gcctttctca agaagttttt
    17821 gaagatacta acatactcta ctttatcatc tttcgacaaa tctctaaatc tatttctata
    17881 cataaaaaaa acatcttcaa attctttttg actcatgtta taaaaactac gattatgttt
    17941 atcactctct atcattactt catgaacacc atatacatct cttgccatat catcaattat
    18001 aggaaattta ttgtagacag attttacaag ccatccttct tcactatcta tttcaaataa
    18061 tgcatcagtt gcatgagctt catttccttt gcaaaatggg cattcttcat catattcatt
    18121 agtttcttca tcttcatttg ggatatctgc tgtatctaaa ggtcttttta acctatctgt
    18181 agcaaatata acaacatcat tggtaattgg atctattctt aattctttca taattaacac
    18241 ctcttagtta atagccttta atataattat atgatatt
//
LOCUS       DAELJO010000065        18224 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELJO010000065 DAELJO010000000
VERSION     DAELJO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.065"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(197..1069)
                     /locus_tag="KPB88_001892"
     CDS             complement(197..1069)
                     /locus_tag="KPB88_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0054172.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1463..2041)
                     /locus_tag="KPB88_001893"
     CDS             complement(1463..2041)
                     /locus_tag="KPB88_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0054173.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2099..2815)
                     /locus_tag="KPB88_001894"
     CDS             complement(2099..2815)
                     /locus_tag="KPB88_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0054174.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTENEIKYLKSIK"
     gene            3006..4091
                     /locus_tag="KPB88_001895"
     CDS             3006..4091
                     /locus_tag="KPB88_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0054175.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFAPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4246..4905)
                     /locus_tag="KPB88_001896"
     CDS             complement(4246..4905)
                     /locus_tag="KPB88_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0054176.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5122..6414)
                     /gene="lysA"
                     /locus_tag="KPB88_001897"
     CDS             complement(5122..6414)
                     /gene="lysA"
                     /locus_tag="KPB88_001897"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG0054177.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6432..7625)
                     /locus_tag="KPB88_001898"
     CDS             complement(6432..7625)
                     /locus_tag="KPB88_001898"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0054178.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(7986..8603)
                     /locus_tag="KPB88_001899"
     CDS             complement(7986..8603)
                     /locus_tag="KPB88_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054179.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(8773..10473)
                     /locus_tag="KPB88_001900"
     CDS             complement(8773..10473)
                     /locus_tag="KPB88_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054180.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(10870..11433)
                     /locus_tag="KPB88_001901"
     CDS             complement(10870..11433)
                     /locus_tag="KPB88_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054181.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11592..12305)
                     /locus_tag="KPB88_001902"
     CDS             complement(11592..12305)
                     /locus_tag="KPB88_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG0054182.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12597..14261
                     /locus_tag="KPB88_001903"
     CDS             12597..14261
                     /locus_tag="KPB88_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG0054183.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14368..15267)
                     /locus_tag="KPB88_001904"
     CDS             complement(14368..15267)
                     /locus_tag="KPB88_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG0054184.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15294..15932)
                     /locus_tag="KPB88_001905"
     CDS             complement(15294..15932)
                     /locus_tag="KPB88_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0054185.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16269..17267)
                     /locus_tag="KPB88_001906"
     CDS             complement(16269..17267)
                     /locus_tag="KPB88_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG0054186.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17365..17940)
                     /locus_tag="KPB88_001907"
     CDS             complement(17365..17940)
                     /locus_tag="KPB88_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054187.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         5883 a         3041 c         1922 g         7378 t
ORIGIN      
        1 aatgaaaata actatgaata aatactctca tttagaagaa atagacaaag atattcctta
       61 aaatttaagc aaaatattgt ttaaataaaa aaactgactg tcagttaaat gccagtcagc
      121 tattttatat attttatcca aacatcacta tatttgaaca ttttaataat taataatact
      181 tattaatata caaactttac tcatacacag aatatctttt ccaaatttgc tctaaatcat
      241 caaaatgctg tttaatatct tctttttcct tcatcccaac agatatagca agagcatttg
      301 aaatactaag agctggaact agagaatcta caaatgaagc catattactc ttaactagaa
      361 gagtgttatc tgcaagtgat gctactggcg caaataagct atctgttaat gatattacat
      421 gggctccttt ttctttagca taattgacaa tttgataaga tttcttagaa taacgaggga
      481 aactaatagc aactattaca tcttcttcat ttattcttac aatctgctca aaagcatctc
      541 ccatatccat tctaattata tgaacattgt caagtattat atctaagtaa aaacctaaat
      601 attgagctac tacaaaagaa ctcctcattc caagtatata tatttttctc gctcttaaaa
      661 gtttgttaga agcttcttta aacgctctct cgtcaatttc ttctagtgta gaccttatat
      721 tatctatatc actttttaaa actttgttta gtattgcaaa atcatcggaa taatcatgtg
      781 ccatttcaac tctttgtact gtagttagtt tattttttat gagttcttga agtgcagctt
      841 gtaatttagg atatccacta taacctaatg catttgcaaa tcttacaact gtagactcac
      901 ttactcctac ggtttcaccc aatttacatg cagtcataaa tgcaacttta tcataattat
      961 ttaaaatgta ttgtgctata agtttttgac ccttactcaa tctcgtatat tgagattgta
     1021 tatttgatat caaatgttta ctgtccttca tttcatttgt ttcttccatt tttttctcct
     1081 tttagttata taaactattt tgttttatta acactgtctt tttctctaaa aattttattt
     1141 tttatctcaa tttcatctta gtttttatca tagcttctta aactcataaa attctaactt
     1201 ttctgtacct tatacttcac atagtatttt cgattaaatt tattatacca tctttccttg
     1261 cattttttaa tatttatcca atatttcttt taaatttttc aatttttact catttaaaag
     1321 ttatctatct tattttgcat atttagatgt atattttgtt ttgagaataa gctgttgtat
     1381 acttattatt taaagtattt ttaataggtc acactatata tattaactct taatacaatc
     1441 ttgtcaagtt ttttagtatt tattatgctt ttttcttttc aattaaaatc aattgtggtg
     1501 ggttattgac ttgatttaaa aacttagatt ctaaaacatt aaattcatct tgatttaaat
     1561 tattgacaag gttatatatg caatcttttt cttcttgacc tccatcatgc cctgtatata
     1621 tggcaatgct tattactcca ttttctttta acatttctaa acttaacttt atagccttta
     1681 aagttgtatc tggtttggtt ataattgaat gtttcgctct tggaagatag cccaaattaa
     1741 ataaaacaca tgaaacttct tcttttatat attgattcat tttttcatgc ccatcaaata
     1801 tcaattttac tctatctgta tatccttctt tctctagttt ctttcttgta gactttattg
     1861 cttcttcttg tatatcaaaa gcatatacaa agcccttttc tccaaccttc tcagctagat
     1921 accttgtatc atacccattt cccatagtag catcaattac tatgtcacct tcatttataa
     1981 tattttctaa ataaaattta tttatatctg taattttagt taaatacttt gcatatgcca
     2041 taatactatt tctccctact attaacttta tttcattttt tctcaattta tttttatctt
     2101 attttattga ttttaaatat tttatttcat tttctgttaa gtatctatat tctccaactt
     2161 tggtgtcttt taatactatt ttccccacag aaatccttct taaatttaaa actggatgat
     2221 ttatagcttt gcacattttt cttacttgtc tatttctacc ctcatgtata gttatttcac
     2281 acactgaata atttttttct ttattttctt tagttactct tattttggca ggtgccgttg
     2341 tgtaatcttc tatttttagt cctgtttcga attgtcttat ctcatcatca cttataattc
     2401 ccttcacact tgccacatac gttttatcaa tttcatgctt tggatgggta agcttatatg
     2461 ttaaatcgcc atcattagta agtattaata acccactagt ttcataatct aatctcccta
     2521 ttggatatac tctttccctt acatctttaa gtatgtctat tacactaggt ctattgaatt
     2581 ggtcttttac tgtagttata tatccctctg gtttatttaa tactatatat atataatttt
     2641 cacttggttt tactttttta ttatcaaatt ctactatgtc tttttcttca tcaatattaa
     2701 aagataactc ttttactaca cttccattta cctttattct tcctccaaga attatttctt
     2761 ctgcttttct tcttgatgct attccacaag aagctatata cttattaatt ctcatactta
     2821 atccttcctt atcttaaatt atgtgtgttt aatattttta tcaatgttaa ttttgctaca
     2881 ttattatact atactagacc ttaattaaaa acaagccaaa aaattattta attttcctaa
     2941 ataattgtaa tttcttttca catactatta atatgtaata gctgagtaaa cactatttta
     3001 aggaagtgat tattatttca aaggaaaata taaaatatta tttaatagtc gtgtttattg
     3061 gaattttatt ttttaaattt attaatactc catcagactt tatatctagt attgaaggat
     3121 tcttaaagtt ttttagccca tttttattag ctattttact tgctttactt ttaaatccac
     3181 ttgtgatgct ttttgaggta aaattcaaag cacatagact tttagccatt tttttatctt
     3241 atatatttat aggttttatt ttagcctttg ccattaggtt attaattcca tcaatagcta
     3301 atacattaaa tagattgata aatgaaatgc ctatgtatac tgattatata gatagtttta
     3361 tagaaaaaaa tatgtcaaac atagattttt taaaaacttt gattcctcat atacaacata
     3421 gtttagataa tttattaaaa gaagcaagta attttgttgg taaaattcct aaaaacttct
     3481 taatctatac acttagtata tcttctatgc tatttaatat gacaatggga tttatactat
     3541 ctatatacat tatatatgac aaagaaaaaa ttgcattggg atttaagaga tttctatatt
     3601 cttctactgc tagaaacaag gcagataaca ttatagaatt ttttaggact actcatgata
     3661 ttttctatga ttatttactt ggtagaatat tagactctct tattattggt ataattgcat
     3721 ttttaggatt tcaatttgtt atcagaatag aaaatgcttt atttttagct tcaataatat
     3781 tcttaggtaa tataattccc tattttgctc cttttattgg tgcaatccct ccaatagcca
     3841 tgactatatt atatagtcca caaaaaacta tatgggtcat tgcatttcta tttatattgc
     3901 aacaattaga tggaaatttt atagaaccaa aagtcatggg taatcaagtt ggtataggag
     3961 ctatatgggt aatatctgct attttggtgg gacaatctct atttggtttc ataggagttt
     4021 ttctttcagt accaatagct gctgttgtaa aaacttatgt tgataaatat atagataatc
     4081 gtttacaata gcttatctct actctgactt taatttttag tttaaaattt aaatgttatt
     4141 tattgaatta tttctcaaaa aatatctctt agtaagaaaa aattgctaag agatattttt
     4201 tataatatat aatttgttca ttaaatttat gcatatattt tatacttatt gattatttga
     4261 gttttgagaa tttccaccac tatctgaacc accagtagta tcttgtggag ttggtgctgg
     4321 tgttggatta ctattatttg gcttttcttg attttgagaa gaactgttat tattgtcaga
     4381 cttattacta tctgattttt tatcattatt tttagaacta gatttattat ttttactact
     4441 tccactataa gaagatttcc ttgcatctga ttcaactcta tacctatctt ctccagccgt
     4501 atcttttgcc tttttctttc tttgttcctc tgcttcgttt tgttcttttt ctaatttagt
     4561 taactcttct ttctttttag ttgcctcttc tttaataaca gaggaaccat ttttgatttt
     4621 ctctataagt ttaagttcct tgattgcacc ctcataattt tttaattctt caaactccgt
     4681 catatttgac attctcatag cttgcatata catagctctt gcattctcat tagtactatc
     4741 ttcttccagt gcagaggata atgattccat agctttttta tagttatgtt tttctaaata
     4801 tgtttttcct tgctctacta aatttatttc atctttttta ttaaatgaac atcctacaag
     4861 aaatatagat aatatcgata ttaataaaat tactcttcct ctcattaaat ccctccttgt
     4921 catatacttt aaattttatc ataattataa agtttttaaa atattttata tttattacaa
     4981 gaaatttgtt agtaaaacaa aaaataagca gatatgctac gaacgataca ataaaccaaa
     5041 aattaattta gagatacata tcctagcaaa tctgctcata acaaacttta atatttaatt
     5101 tttattatat acttctccat tttatatctc taaagctaat aagtcctcat agctttgtct
     5161 cttacagatt atcttatcat ttccatcaaa tactgatact actgctgctc ttggtatttt
     5221 attataattt gaagacattg aatatccata tgcaccagtt gatgttgtta taagtatatc
     5281 tccactttta atatccatta tgtttatatc tccaattaat atatctccac tctcacaaca
     5341 ttttccagct attgttacat gattcatagc ctcatgatta attttattca caatactaca
     5401 ctcataacta gcttggtaca acgaaggtct tacattatct gtcatacctc catctacact
     5461 tacataaacc ctaacatctt ttatatcttt tatagaacct actgtataca atgtacttcc
     5521 tgcatttgca actattgatc ttcctggttc tattaccaat gtaggtactt ctattccaag
     5581 ctctttacat gaagtttctg ctttagtgat tatggtttca caaaactctt ttatagtttt
     5641 aggtttatct ccttcgctat aataaacgcc aactcctcca cctaggtcaa tttctgttag
     5701 ttgtatgttg aatttttctt ttatttcttt taccaagttc atcattatat ccactgtatc
     5761 tatgtatggt tctacatcaa atatttgtga acctatatga gcgtgaagac ctacaagttt
     5821 aatattttta tattcactta atttttctat tgctctaaat aaatctccat ttactaaagc
     5881 aaatccaaat ttagaatcta tctgtccagt ttttatataa tcatgtgtat gagcttctat
     5941 accaggagtt attctaaagt atatctcttg agttttacct ttatcttcac ataatgcttc
     6001 aagcaaatct aattcataaa aattatccac tacaaattta cctacaccta aatctaatcc
     6061 catgtttatt tcttctttag tcttattgtt tccatgaaag aaaacttttt ccataggaaa
     6121 atttgattta taagctgtat ataactcgcc tcctgaaact acatctaaac acatttgttc
     6181 ttcatttata agattacaca tgtaaagagg taaaaaagct ttccctgcat aagcaacctt
     6241 attttttccc tctctagcct taaaatattc tatatattct ctacaatttt gtctaactaa
     6301 agcttcatca aatacataaa gtggagtttg atatttttta ctaagttcta aacaactcac
     6361 tcctccaata tataattcat cattatttac agtcatagtt ccatgaagtt tcataatatc
     6421 ctccccaaat tttatatatt aaatacttct gctatttttt ctatagctac ttgcttatca
     6481 tcagaattta tagcacaagt tatacgaatc tctgatgtag ttataagttt tatcattata
     6541 ccattttcat tgaatatctt aaatacttta gcagcaactc cagaagtatt tttcatacca
     6601 agacctacta aagaaaattt agtgatatta ttatcaatca ctacatgttc ttcatctgta
     6661 tatttagata ctatcttttt acattctccc aactcttctt ttggaactgt aaatgataca
     6721 cttatcttat ctaaaatagg tgcagtttgg cttatcatat ctacacttat acctatagtt
     6781 gctatatctt caaataagct agatatattt tcagctttaa aatcttttat tgttatagaa
     6841 ctgtcatcat cactagtagc taatcctgtt attactttat cctctaattt catgtctttt
     6901 ccccctctta tatatgttcc tttttctgta ccacatgtac gtccgacata gatttcaaca
     6961 ccatattttt gtgcaagctc tatacttctt gaatgcatta cttgtgctcc taaactcgct
     7021 aactcaagca tttcttcata ttcaatctca tcaagtttac tagcctttga atattttctt
     7081 gggtctgtaa aataaattcc atcaacatct gtatatatct cacattttcc atttaattta
     7141 accgcaagag ctacagcaga tgtatctgaa ccacctcttc caagagtggt tacatcacca
     7201 tcttcgttga ttccctgaaa tccagttact atcacgacat ttccttcatc taaactattt
     7261 ttaattctgt ttacgtttat atcatcaatt tgagacttcc catgaagacc actcgttttt
     7321 atatttaact gataagcatt ataacttata gctttacatc caagtgcatt taatgctatt
     7381 gataataaag atgctgaaat catttcacca gtagacatta aagcatctaa ttccctctta
     7441 ctaggttcat tactcaattc ctttgctaaa gttatatatt catcagtgga ttttcccatg
     7501 gcagacacaa ctattaccat ttgtggattt tcttttcttc gttctattat attttctgct
     7561 attgatttta ttttttcagt atctgctact gaacttccac catatttttg aacaattata
     7621 ctcatgcttt ctccccctca agctaaattt tgataaataa aaacgcccaa aggtatactt
     7681 tggacgtgta aatttcatag accaagtaac cttgtagctc accactttaa aagtgacagc
     7741 cttatgcata ttctgcataa gtccagaaaa tttgtccgtc aacaaaattt cttcggctat
     7801 ataccctttc atcaattatc aacgcttacc agctcctatt gaatactatt gaatacagct
     7861 acctctaccc taggctcatt atttttaatt aatttattaa tatagtttta tcatattcta
     7921 tataatattg caacaattat tttacaaaat gtggcaaatc gatagttttc ttttctaaag
     7981 ttagcttata tacaattaaa tagtttattt aatcctaaat ctataaaatt tatgctatac
     8041 tcattatcat atctctctaa atcatttaat tcggatagat tatctggtgt aactaccaaa
     8101 acgtttggta ctttaaaaaa tggaattgta gatttatcta tttttttaga aagattttca
     8161 tatttagaaa tgtctatttc attatttaag tcaacatcaa ctaaaacttg ctttatatta
     8221 ccaaattggt ctgtatattc aataaataaa tcgtatttca catcatctac actaacattt
     8281 cggtaaaatc tttttacatc acaacctgca gtattgattc taacaactaa ttctgatcct
     8341 actagagcct ttattaaatc atctccctta aaaagtttac taccgtttaa atagtataca
     8401 taagagaggt ctttataatt ataataatcc acatcacaag gatatttttt tagtgtatat
     8461 tcactacaca tttttctcaa agttttttta aaattttttt tattgtatct tttttggtct
     8521 ccataaattt ttctaaattg agactctgta acacatctaa ccctgctaat taaaagtaat
     8581 atctcttttt ccatattcgt catagtacat tctccttgaa gtattatttg caaagttgta
     8641 ctaatattat attaatttta tgacattaaa tttacaaaga attaaaataa aatagctaat
     8701 ttttatagaa ttaaattcta taaaaattag ctatcactat gttaaatact attatcaatc
     8761 tttttattta aattatacat taccttgatt ttcagaatct ttggaatctg tattttcatc
     8821 agttcctgtt ttttcttttg cttttttatc ttcaaagttt gcaatattta ggttatcaaa
     8881 ttctgacatt tctattacac cagattcttc ttttgaatac gatattttga ctttatcacc
     8941 aacctgagtc aatggcaatt cgctagatac ttttgaagta gccctaaata ttatatcttt
     9001 atttgaatct aaagttacat aataattagt gttaccacct cgcacatctg ggcttattct
     9061 tgtcactgtg ccttctaata cctcatcttt taaatcatta tcaagtttta aatcattacc
     9121 cttagattct aatgcatcct tatagtttct taatgcttca ttcttatctt cacctattcc
     9181 aagtacatta taatcttcaa cagatacaaa tgcaactctc ttaacaagac cagccttatc
     9241 ttttaatgat gatacatatg ttggctgacc caatatatta tacattacag ggaacgtcgc
     9301 ttcataattt ttttcttgaa cattaccttc agctgatttc attgcagccg tttcagtagc
     9361 tcctggttgt ttatataatc tagcttcttt tgttctagaa tcaactaaca taaatccaat
     9421 tgtagattca tctccacctg aagatgttat acctgtatac caataagacc tgttatcatt
     9481 gccataaact aatgatgtac cttctgttgg tactaaaact cccttttctg atataacaga
     9541 gtttaaaaat ccattaacat aaacacccca gtctttaatc tggtctgtta caaagctttg
     9601 tggttgtatt ctatccaccc atttaggtgt gtttttaaca tcataaacat ttatttttcc
     9661 agtttctgca tcaacagtgg ctattcctgt tgcatttgct cctccatagc caattttatg
     9721 ttcatataaa cttacaaccc aataaggtct tccttcatca tttatttcaa gagtgaaatc
     9781 tgtcatacca acatttacaa taccatgtat gtataaatgt ctttgtaaat cctgatgtaa
     9841 gtaggcttct ggttggtaaa ctatctttat tggctttcca tctatttctt gaactaattg
     9901 gacgtcttga ggattactag ctgatacttt tacataacca cttgtaccac ttagagatgt
     9961 tatccattta attatgtctc tgtgaacaag aggcgctacc cagtacaact ctccatctac
    10021 attttgtata tgaaattttc ctaatttgga tacactccct attgcaggaa cttctccaag
    10081 ctttttgtca cctaatttca ttgccatatc ttcatctaca agacgaatgt catttacact
    10141 gacagggcta atgtcatcag taaatttact ttcagttact tttcccaata gttctctata
    10201 tgattttgca tgaagtacag gtgtagaagt tataaaggga actactacaa tatacacaag
    10261 caaagctatt gctattgaaa aattatattt agccatttta gatgctcttt catttctatc
    10321 taacatcata tctagtattc ctgctacaac aaagaaaatt attcctactg aaaataaaga
    10381 tgtaaaatct aatcttagta caggtaattt aataaatgca taaattatta caattattaa
    10441 tgcaactcca tatgttttaa gatatcgttt catttaatac ccctctctaa aaaatgttat
    10501 tattactatt atatccatat atttacaaaa aataaatatt tatcatattt attatatatt
    10561 aagatatctt tatatattta tagttctgct tattcaacta tatttaacat tatacgtgta
    10621 tgtataaata ctttttatac actgtttgtg ctatccatcc cgcacctaaa ggagtgggat
    10681 ttttgcacta ttctgtatta tatatatcca aaaatgttat aaaaatacaa atattagcag
    10741 aaatttaaaa actgaagaaa gtattctaac taagatttac aaattcttaa tcctatcgaa
    10801 gttaaactac tatcttcagc ctaattatct agcaatattt ttttatatga aaaactctaa
    10861 atcaagtttt taaatgtttg cgatttttga tacataagaa tgttgtctag aattatattc
    10921 tgataataaa ccttcatact ctaaggttaa gccagataaa ttataaaaat catttggcgt
    10981 tacttcaaat gcttgtttat ttaaaatgtc tatctcactt ttttctaaca tgtcagcaac
    11041 aaaatttgaa catacatatt tatattcaga ttgtctctga atattcatta gtaaatatat
    11101 taatgccatt gtatcgtaat agtaatcttc tctataatca ctaattaatt ttatattttt
    11161 atatagattt tcatattgta aattatctac ttctaacgaa tataccctac acacagtgtt
    11221 ttttcttata gcatatagtc cttgatttat attttcttca acaaatcctc caataaatgg
    11281 attccttgga tttagccttc caaaagaata cattggcttt agattttcat ttaatgatat
    11341 tgacacatgt gtatacagct ttttacttat attccttatt aagtaagata atatagttcc
    11401 tgtatatgtt gttacaatat agactttatt catcatttcc ccccaatcta gtaactaaat
    11461 aatattatac acttacaatt taatatcata agatattcta acataataaa tgtggtttta
    11521 caaattttga ttttaaggta ctttaatatt atataatata ataaaaatat aaatataagt
    11581 tttctatatt tttaaccaac agatgttttc ttcaaattaa tcagatgatt tagtatatct
    11641 tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga ttttaaatct
    11701 atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt tttaagtgtt
    11761 attgggtaaa gataactttt ttccaaagta atcttatttc cattaacctc aaatggaact
    11821 tttttattgt agttacttaa tattttttct agcattttat cacttagttt aaattttcca
    11881 attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg tctcaattca
    11941 agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac ctttatttta
    12001 ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt ctgcatcaat
    12061 gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac cattctaagt
    12121 ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata ttcttcatct
    12181 atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa gatatataca
    12241 acaattcctg caagtactaa aacaataaca attaaactta acaaaatctt tgcaaccttt
    12301 cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta gataactaac
    12361 ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa attaattcaa
    12421 ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa agttaatatt
    12481 ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa aattaatatt
    12541 atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta tagaatatgt
    12601 caaatgaaac gaactcatct aactttataa agaatattat tataaacgac ttagaaacag
    12661 gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat ttacatattg
    12721 gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat ggaaaagcta
    12781 atttaagatt tgatgataca aacccattaa aagaggatgt tgaatatgta gagtccataa
    12841 aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca tctaactact
    12901 ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct tatgtatgtg
    12961 atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca ggaaaagaaa
    13021 gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga atgaaaaatg
    13081 gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct tctccaaata
    13141 taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat aatacaggag
    13201 ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat gcaattgaag
    13261 gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt tatgattggt
    13321 ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct agacttaata
    13381 taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag ggtattgttg
    13441 atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag ggatatactg
    13501 cagaagcact gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac tctacagtag
    13561 atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct cctcttacta
    13621 tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat aagattgaga
    13681 tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta gttcctttct
    13741 ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag aaatatttta
    13801 gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg
    13861 taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac cctgaaacta
    13921 agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg gttgatgcta
    13981 aatcagctat accatgtgaa tttagattat ttgaaccatt aattcttgat gatatacctg
    14041 aaaatgaagg taaacatttc ttagaacaaa taaatcctaa ttcattggaa atattacaag
    14101 gttttgttga acctacacag ataaaagatg ctaaaccttt tgataaattc cagtttgtta
    14161 gaaatggatt ctttagtata gataataaat atacaactga tgaaaaattt gtatttaata
    14221 gaatagttcc tctaaaaagt tcttttaaac caggtaaata atcttacaat caaaaaatga
    14281 agacttaaaa tataaattac ttctactata aataaactta gttagaagta attttttatt
    14341 tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc ccatgacttc
    14401 tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt ctccgtcaat
    14461 atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa agtatagtat
    14521 ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca aaactggtat
    14581 tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat catctatttc
    14641 agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca acataagata
    14701 catatctcca tcataataca tttcctcaga agatactttt atcttaaatt tcctcatata
    14761 caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat tttttaagtc
    14821 tgtgtttatc ctttgtgata catccgtaaa cataccagca ctcgcaacat ttataaagta
    14881 cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga tttcatttat
    14941 agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg cagttcctgt
    15001 aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta ctactaaatc
    15061 tacagtacca tcaccaccag ctatcaatat gtgttcataa gaactatcaa cttccttaaa
    15121 tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat aatttttttc
    15181 ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac ctgaattagg
    15241 attgtatata agttttactt ttttcattat ttctccttac tcattttatt cttttagtat
    15301 ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg tctttttata
    15361 agtgtataac tacagcatat tcttcctgga cttgaacaat ctatacatat accagtagtc
    15421 ttacacccag tatttgtact taatctttta caattggctg gagcagctat tgtttttgtt
    15481 ctttttatag cttcatctac atctttaact attttattta ctcctgctat aactattact
    15541 ttatcaggtc catatatcat agcagcaact ctattcccat tcccatctaa attgtaaagt
    15601 tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga aaatgacttt
    15661 ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc aagaaaatta
    15721 tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga acctccacat
    15781 cctactagag aacctgcttt aactatttct tcaatttttt taagaagctc ttctttgtta
    15841 cttactaaat accctttcat attattgttt tccaaagatt ttattgtttt ttctattctt
    15901 ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt tttaatatgt
    15961 acttaattat ttataaactt tattttcttt ataagtttat tttagtacat ctaaaaaaaa
    16021 atacaatata aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa
    16081 aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat ttattgccta
    16141 aaatctatat attaattata tgtttcatat tatataatat cacagttaat taaaatgtaa
    16201 tactatcttc tttttaaaca tataaattgt attattaaat ttaatatatc caacttcaat
    16261 aaatcttatt aatttgatga agaagatgct atagctgcac ttgttgctgc cattacacat
    16321 gcaatttgaa tagcaacaac aatatttaaa aatatattat tagttataga gcctatatta
    16381 ttctcatttc gtatcaaatc tgcataaatt gaagctacaa tagcagaact aatcattact
    16441 ctattacttt ttcctaaaga ccaatttcca aagccttttt cttctttaag tttatttgaa
    16501 acatttttta tctcttctat tatttcatct tctctataat ctaataatga aattgctcct
    16561 attattggat atccataata attcatttta caatcatttt ctttaaatgc ttttttaagt
    16621 tcaatagtct ttttacattt aagcatagtt ctatcatcac taaataaaag aatttgggat
    16681 aatgcttgta aatcatttcc tttataaaaa ccattttcat ttagaaattc ataacatttt
    16741 tctatgtatt ctaaatcttc atctaaattt tttgaatgaa ttgcaataag tgctatatta
    16801 caatagtcat cactagaagt tagaaatgga tgttttttcc tcatgtaatc atatattatt
    16861 ttcatttttg atatacatgt aagataatcc attttctctc tattttcata aactattgat
    16921 gctgtaagtg gtaaatatgt atcgccccaa aacttttcac ttttaagttc tttgtatata
    16981 tctaaaatca tcttaaaact actttctgga tttgattcaa acgaaagtag tgttgccata
    17041 taaaacatat tatatcctct aaaattagag aataatccag tatttttttt aataagctct
    17101 attcgttctt ccactaatct aggacttatt ctcttgttct taagggtaaa tgcaagtgca
    17161 caactatgtt gtatcaatcc catttgccaa gaacctttaa cttcatttaa tgctttgtaa
    17221 ttttctatta ataagtttac ttttccattt ataaacgatt gttccatatt gccctcctag
    17281 taatatatct cattgtacta atagttttat atttattata tatatattaa atactacaat
    17341 caatacatat tacttcaaag aacatcattc ttcaacaata atgtagcctt ttttgtaatg
    17401 tcttcccata ttattgactc tatcatagta ctctgattca ctttcattac tccatttggg
    17461 cacaaataca tctattgcat tagaattact aaaatactta ctagaccccc tatccataac
    17521 agtttttaat tttccatcta caataatctt agttccaaaa cttaagtgat tgtttgctac
    17581 aaaaccatct tctaaatcat ctcctgtagc agttatcgct ccatatccat tttcttgatt
    17641 aagacttgtg taataactaa cttcaaaagt tataggtact aacttcttta ttttaatacc
    17701 taactgattt tgtagtttct ccttttgtac tttcaactta tactcatcac tatttttata
    17761 atcttccata ccttgattat acttggaatt aaagctttta cctgacttta ctatagtatc
    17821 tatgtttact atagattctt tacaatgatc tgcatctatt tttgtttctc catttaccaa
    17881 taatggagtt aaacatacac tacatacaac acatgtaatc aatgcttctt taagcttcat
    17941 aaattccccc cttattattt tgatatatta gcatatttaa tattatatgt ctacatttag
    18001 atttaaatac cacacccatt tgctttaaga agttgtctta tatttaattc accttaaaca
    18061 cttataaatc ctctatctta gaatcatata gaataaagct tttatcatta aatatttttt
    18121 attaatcttt taaaatctaa aactacttta tctaattttc tcttttttag aatttaagaa
    18181 ttaaaattct ataattttaa aacatattga atttattttt ttga
//
LOCUS       DAELJO010000066        17723 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELJO010000066 DAELJO010000000
VERSION     DAELJO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.066"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            203..3751
                     /locus_tag="KPB88_001908"
     CDS             203..3751
                     /locus_tag="KPB88_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0054188.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSVKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSKGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3936..5870
                     /locus_tag="KPB88_001909"
     CDS             3936..5870
                     /locus_tag="KPB88_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG0054189.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGPRCLIKPKNEKPYFNRNNSKKNVSKETNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5976..6971
                     /locus_tag="KPB88_001910"
     CDS             5976..6971
                     /locus_tag="KPB88_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0054190.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6943..7728
                     /locus_tag="KPB88_001911"
     CDS             6943..7728
                     /locus_tag="KPB88_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0054191.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7716..8414
                     /locus_tag="KPB88_001912"
     CDS             7716..8414
                     /locus_tag="KPB88_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054192.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8537..9010
                     /gene="ybaK"
                     /locus_tag="KPB88_001913"
     CDS             8537..9010
                     /gene="ybaK"
                     /locus_tag="KPB88_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG0054193.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9207..10046
                     /locus_tag="KPB88_001914"
     CDS             9207..10046
                     /locus_tag="KPB88_001914"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG0054194.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10059..10607
                     /locus_tag="KPB88_001915"
     CDS             10059..10607
                     /locus_tag="KPB88_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG0054195.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10673..11854
                     /locus_tag="KPB88_001916"
     CDS             10673..11854
                     /locus_tag="KPB88_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG0054196.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12149..12535
                     /locus_tag="KPB88_001917"
     CDS             12149..12535
                     /locus_tag="KPB88_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0054197.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12685..12948
                     /locus_tag="KPB88_001918"
     CDS             12685..12948
                     /locus_tag="KPB88_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0054198.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13077..13919)
                     /locus_tag="KPB88_001919"
     CDS             complement(13077..13919)
                     /locus_tag="KPB88_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0054199.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14214..14948
                     /locus_tag="KPB88_001920"
     CDS             14214..14948
                     /locus_tag="KPB88_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0054200.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14958..16091
                     /gene="nagA"
                     /locus_tag="KPB88_001921"
     CDS             14958..16091
                     /gene="nagA"
                     /locus_tag="KPB88_001921"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0054201.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     AFEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16190..16939
                     /gene="nagB"
                     /locus_tag="KPB88_001922"
     CDS             16190..16939
                     /gene="nagB"
                     /locus_tag="KPB88_001922"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG0054202.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17114..17386
                     /locus_tag="KPB88_001923"
     CDS             17114..17386
                     /locus_tag="KPB88_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054203.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         7096 a         1860 c         3118 g         5649 t
ORIGIN      
        1 gttataattt ttaatattaa ttaagaatga tgaaatatga ataaagattt tacaaataaa
       61 tcttaagcta tcttatttaa ttctaataca ggaagaaggt aaatgtatat tgaaaaaatc
      121 atagaaatag aagtaaacaa tttaaataaa tacaaaggtt tgcaaaagaa gtaatataat
      181 tatatacaat gaggatgtga gtatgatgaa cagactaaag ccaagaattt taggtattag
      241 tatcttgttt attattataa ttattttaac atgtggagct gttttcaagt ttaaatttaa
      301 attagataat aaattaaaag aaaccacaca tacatatttg tctgagttta cagaacacaa
      361 tataattaat ctcaagacaa ggcttagtgg tcaatttgac atgcttgaat ctattgctaa
      421 cttcatagga aacttgaata atataaatga tgaaatggtg attgatttaa tggattctga
      481 tgtaaagcgc agttctttaa ttagaatgac tatctctaca ttagatgggg aatgttattc
      541 taatgatgga attacccata atgttcaaga tagagattat tttaaaaaag ctaaaaatgg
      601 agaaaagaat atatcagaac ctttaaaatc tattgttaat aataaagaaa taatagtttt
      661 atctacccca gtttataaaa gtgggaaaat agttggtgtt ttaagtggaa tttatgattc
      721 aaaaaagtta aatgagttat taggattatc tgcatttgat gaaaaagcag atgtttttgt
      781 tgcaaagaaa aatggagata tagttgctca aaaaacaaat aaaaatagaa agacgctatc
      841 atgtatattt cagctttttg acaaagataa ttgtgatgaa gatgattgtc taaaaactat
      901 aaaagaaaat atgaaaaatg ataagtctgg atattttaat tataacttag ataatgaaaa
      961 atttatgtta aactatcagc cgcttggagt taatgattgg tatgtttttt ctgttgtgcc
     1021 aaataaaata gtatctattc agtcagagaa tatatcttat gatgtatata tttttgcatt
     1081 agaaatacta ctagttatat tgatttttat aatatatata gcatgtatta taaataataa
     1141 caccaaaatt attataaatg aaaaagaaaa atttaaagta ctcactgata atatattagg
     1201 aggggttaaa acctgtttaa aagatgaata tatgactatg acatatgtaa gtcaggggtt
     1261 tttagagctt acaggttact cttgtgaaga tttaaataaa atatttaaaa atagatttaa
     1321 agatatgatt tataaagatg atttagacaa gtttaattgt attattctaa atcaattaga
     1381 aaaacaagat aaaattgaag ttgaatatag aattgtaaga aaagatggaa gtataatttg
     1441 gattttggag agaagtaagt ctataaatga taataaagga agtgagtgta ttcagggagt
     1501 tttaactgat ataacagaac ttaagaaaat acaagaagaa ttagattcaa aaagatttga
     1561 aatacaggct atcaatgata gtattgttgg tggaatagtg ataactgaga taaatgatga
     1621 ttttgatagt atatatttaa atgaaggtta tttaaatatg attggatata cacgcaaaca
     1681 actggaagat gagctaaata ataaattaat caaacttatt tttaaagatg atagaacaga
     1741 attaattaat agtataaaca aacaactcaa attaagtgat acatttgcat ctgaacaaag
     1801 agttgaaaaa agggatggca ctattatatg gattttatta aaaggaagaa agatgattta
     1861 tgataatgga aaatctgtac tttgttgtgt tattgtagat attacttctg ttaaattatt
     1921 agagcaagaa cttaaaaaga gtgaagaacg tcatgaaatc attttaaagc agacgcaaaa
     1981 tattataata gaatgggata tagaaaaaga taaaatgaat atatcaccta attggtataa
     2041 taagtttaaa tatgacccta taactgaaaa cgcaacaaaa aatataatta aatcagtaga
     2101 tatatatcat gatgatatgg ataagtttat ttcattaatt aaacttactt caaaaggtag
     2161 tgaatatgaa gagtgtgaaa ttagacttag aactagagat actatttacg cttggtttag
     2221 tataaaggca acaactttat ttgataataa tggtaaacct tataaagtag taggaagttt
     2281 gactgatatt gatgaatcaa aacgagaagc tgaaaagttg aaagaaaggg cagaaaaaga
     2341 ccctcttaca gatttgtata ataaaaaagc atcacaatca cttatagaag agtacataac
     2401 attggataaa aaaagaaaag gtgctttaat gattattgat attgatgact ttaaaggaat
     2461 aaatgataat ttaggacatt tatatgggga tgctgtatta agtgaaattg caaatgacct
     2521 aataaatcta tttagagcaa ccgatatagt aggaagaatt ggtggagatg agtttattgt
     2581 atttataaaa gatatttctg aaacaagaga tattataaat aaagcagaag aattggtaaa
     2641 aacatttgaa aggtcttttt taggtaaagg acgaaattat aaaatatcat taagtgtagg
     2701 aattgctaag tttccacaag atggtatgga ctatatgaca ctatttagaa atgcggatat
     2761 agctctttac tcagccaaag ggaagggtaa gaattgttat acattgtatg ataaaaaaat
     2821 tgataaagtc caatatataa ctaatacaag aaatgcagga gatagtgata atgtagagat
     2881 gtcttttaaa gaacatatca caaattatat atttgatata ctatatgaat caagtgatat
     2941 agaagtggca gtaaatttaa ttctaagtat cgtaggtaaa cactttaatg tgagtcgcat
     3001 gtatatattt gaaaattcag atgataatcg agtatgtaaa aatacatttg aatggtgtaa
     3061 cgaaggtgta acctcggaaa ttaataatct taaaaatatt tgttacaagg atttaggcaa
     3121 ctatctttta aattttaatg cagatgggtt attttattgt aatgatattg aaaatttagg
     3181 tagtgatgtg aaaaaagtac ttaagccaca aggcatacgt tcaatattac aatgtaaaat
     3241 tttagagggt aatgaattta gagggttcat aggatttgat gaatgttcat ataatcgtat
     3301 gtggactaat gatgaagtga acactttgac atttatatct aaaacgctca gtatgttttt
     3361 attaaaaatg cgtattcaga gtaagctaga agagtcttta acaattcaaa aatctgtgat
     3421 ggataatatg gatatatggg catatgttgt tgacaggaat acctatgaat tactatttat
     3481 caataaaaaa atgcttgagt cagcaccaaa cactcatgta ggtgatttat gttataaagc
     3541 gatatggaat aatcaagata aaccttgtaa aaaatgccct atgatatatt taaaagatgg
     3601 tgatgatact tataaatcat tcatgcataa taaaaagttt gaggtacagg caaatataac
     3661 tacaactact ttgaaatggt ttgatggtaa agatgcatgt ttaatatttg gaagtgaaat
     3721 aactagtatt gaagctgaag aaaatactta aaatactgtg ttatatatat acttaaaatc
     3781 gcaatttgaa tttattagac ttaaaatgtc tatataaatc atttaagttg tggttttaag
     3841 tattttttat gatataatta agcgttaaga acaaaattaa aagatacttt tgtataaaaa
     3901 aatatttgtt aaatatatat tgagcaggtg ataatatgga aaataagttt ttaccaataa
     3961 gcaaacaaga tatgatagat agaggatggg aagaattaga ttttgttcta gttacaggag
     4021 atgcttatgt ggaccatcat agttttggaa cagcaataat atctagagtt ttagaaagtg
     4081 ctgggtataa agttggaata atagctcaac cagactggag aactacagat gattttatga
     4141 agctaggaaa acctagactt gcatttctag ttaatgcagg aaatatggat tcaatggtca
     4201 atcattattc tgtaagtaaa aagcatagag aaaaggatat gtattctcca ggtgggaaaa
     4261 tgggttatag accagataga gctactatag tatattgcaa taaaataaga gaagcataca
     4321 gtgatgttgc aatagtaata ggtggaattg aagcgagctt aagaagattt gcacactatg
     4381 attattggag tgataaggtt agaaagtcta tgttaataga tagtggagca gatttattag
     4441 tttatggtat gagtgaaaag cagattgtag aagtagccaa tgcacttaat gatggatatg
     4501 acccaaaata cattagacat attgatggga catgctatat ttcagataca ttggaagaaa
     4561 tatatgataa atatatatta attccatcat ataaagaaat ttgtgaagat aaaatgaagt
     4621 atgtagaagc ttttaaaatt caatatgatg agcaagaccc atttagaggt aatataattg
     4681 ttcaacctca tggaagtaag tatctggttc aaaataagcc agaaaaacca cttagtagag
     4741 aagaactaga tgaagtatat ggattaccat atcagaagac ataccatcct gtgtatgaaa
     4801 aatttggagg aataccagct atagaagaag taaaatttag tatagtaagt tcaagaggct
     4861 gttttggtag ttgttcattt tgtgcaataa catttcatca aggtagagca gtgcaaagta
     4921 gaagtgaaaa atcaattatt gatgaagcaa taggaataac aaatttagat gattttaaag
     4981 ggtatataca tgatgttgga ggtccaacag ctaattttag aagaccagca tgtaaaaaac
     5041 aaattactaa aggtgcttgt aaaaatagac aatgcttatc tccatcacca tgtaaaaatt
     5101 tagatgcaga ccacagtgaa tacttacatt tacttagagc tgtaagaaaa ttacctaaaa
     5161 taaaaaaggt gtttgtgcgt tctggaatta gatacgatta tgtaatggca gataagaaca
     5221 ataaatttct tagagagttg atagagcatc atgtaagtgg tcagttaaag gtagccccag
     5281 aacatatatc agaagaagtt ttaaagtata tgcaaaaacc agcaggaaaa acttatgata
     5341 aatttagaca aaagtttttt gctataaatg aagaattagg taaaaagcaa tatttgatac
     5401 cctatttaat gtctagtcac cctggttcta ccttaaatag cgctatagag ttagcagagt
     5461 atttaagaga tactcactac caaccagagc aggttcagga tttttatcca actccaggga
     5521 ctctatctac aaccatgttt tatacaggta ttgacccgct tacaatgaag ccagtttatg
     5581 ttcctaaatc aaaacgtgac aaggcaatgc aaagggcttt attacaatat agagcaccta
     5641 gaaattatga tttggtatac tctgcgttag ttgaagcagg aagagaagac ttaattggtt
     5701 ttggacctag atgtcttata aagccaaaaa atgaaaagcc ttattttaat agaaataatt
     5761 ctaaaaaaaa tgtatctaaa gaaacaaata aaaacaaaaa aactaataca aataacagaa
     5821 gtaataaaaa tcaacaaaaa agttcaacta aaaaaaagaa aaaacattaa agtataaata
     5881 aaaatactta aaaaagaagg attattcgat aaattaacag aaattagtat aaataaatat
     5941 aattatttta atgacgttac ctttgggagg aagagatgaa aaagtatatt tctataatat
     6001 tattagtggt gttgactatg gttgttttgg taggatgttc accaggaaaa gataacccta
     6061 aaaataagga gctatcagtt gtcaaacaag taaaagtagc tgttccagat ggactaccag
     6121 cagtggcaat agctaaactt gcaaatgaaa atccagagat taaagaagga tatgaaacag
     6181 tgtatagcat tgaaaagaca cctgaagcga tttccactag agtgatgaaa aaagatgcag
     6241 atatagctat agttccttca aacatggctg caatagcgta taataaaact tcatcatata
     6301 atattgttgg aactgtagga atgggttcac tttatttggt gtcaacagaa aatataaaag
     6361 attactctga tttagttggt aaagaagttg gatgtactgg aaaaggactt actccagaca
     6421 taactataaa aagtcttttg ttacaaaagg atataaatta ttcagatata aaatttaatt
     6481 atgtaaattc agcaagtgaa ctggtaccat tattggctac tggtaaagtt aaaacaggta
     6541 ttgttccaga acctgcactt agtgctttga tgtctaaaaa tccagacata aaaattataa
     6601 aaagtttaaa tgactcatgg aaggaagtaa gtggttcaaa agatggttat cctcaatcta
     6661 ctttaattgt aaagtctgaa tttttaagag ataataaaga ttttgttgat tcttttgtag
     6721 gtcaattatc aaatagtata gattgggcaa acaaaaatcc tgaagaatta ggtgcatatt
     6781 ctgaaaaaat aaaaatatct actgaatcaa agataatagg aaaatctctt gaaagagcta
     6841 atttgaaata tattccagtt aaggacatga ttaaagatta taaaaattac tatgagaaat
     6901 tagctaattt tgatgataag acattaggag gcaaagtacc agatgaagca atatactttg
     6961 ttgagaaata aaaaaaataa agaacaattt atagataaaa tggaggtaac actttcttgt
     7021 gttatcctct tatttttatg gcaaattatt gctttaaaaa ttgataatga tattttttta
     7081 ccaacagtag gtcaagtatt taatagtata aaagagatta tgctaaattc tagtttctat
     7141 atagatatac tttactctat ggggagatgt ttttttagtt ttttattggc tatgattttt
     7201 gctattatac tttcaatagt gtcttattta aatagatttt ttagaaactt attaaaacca
     7261 atcaatgctt tgacacgttc aataccaact atgatattgg tagtacttgc acttatttgg
     7321 tttgaaaagg atagtacccc ttttatagtt ggatttacta tagtttttcc tatattgtat
     7381 gataacgttt tgggcgctat attgaatata gacaagaact tattagaaat ggctaatgtg
     7441 tataaaataa ggtttataga taaggtgtta aaaatttatt taccagccat aaaatttcaa
     7501 attatatcta tactagtatc aacattctct ttaagtttga aagttgctat agcaggagag
     7561 gtatatagtc aacctacata tggtattgga actatgatac agactgaaaa aattaacttt
     7621 aacacatctg gaatatttgc atggataatt attgttcttt taatatctgc tattttacaa
     7681 atagctcaaa agtttttagc aaggagagct tttttatgga aaagataaag ttgagtattg
     7741 aaaatataaa taaaagatat gatagtcgta ttatatttag agattttaat attgattttt
     7801 atgtaaatga ggtaaattgt atccttggaa aatctggttg tggaaaaact acactcttga
     7861 acataataag tgggattatt aaaaatgaca ccaacaatct aaatatcaaa gaaaatttaa
     7921 atagagtagg aaataagctt gaagctagtt acatatttca agatgataga cttatagact
     7981 ggttaacagt agaagaaaat ataaagatag ttgtaaataa atactataat aaaactcaat
     8041 taaataaaat ttgtgatgaa tacttggaat tggttggaat tagtgattat aaaaaattct
     8101 atcctcagat gcttagtgga ggtattagac aaagagtaaa tattgctaga gcttttatat
     8161 atccatcaaa aaatattata atggatgaac cttttaaatc tattgatgca aagaatacac
     8221 agcttataat ggataatttc agaaatatat taagaaaaga aaaaagaact gtgttatttg
     8281 taactcataa tattgaggaa gcattgtttt tagcagatag gatatttatt ttaggagact
     8341 ctccaattag aataaaaaaa atattaaaaa attctaagga attggaaaaa aatgaagttt
     8401 tgaagttgat atagagttat aataatactg tcgttttaaa atatatttgg ttaaaatcta
     8461 taagactata ttaaaatagt acagaatatt caaaactgtg tataatgttc gtattttata
     8521 aaaaggaagt gaatgtatgg ttaagactaa tgctatgaga atattagatt caaataagat
     8581 tgattataaa gtaatgtctt atgaagtgaa aagtgaacat gtggatggtg ttgaggtggc
     8641 acatgatata ggaagagatg taaatgaagt gtataagaca ttagtaactc aaggagttag
     8701 taagaatata tatgtatatg taataccagt acatgaaaac ttagacttaa aaaaggctgc
     8761 aaaagtagct aaagagaaaa gtgtagagat gattcatgta aaagatataa ataagcttac
     8821 aggttatatt agaggcggat gttctcctat aggtatgaaa aagctttata aaacttttgt
     8881 aaatgaaagt gcaaagaatt tagatacaat tattgtgagt gcaggaaaaa taggatatca
     8941 aatagaacta tctccatttg atttacaaag acttataaaa gtagaatttg tagatgtaat
     9001 aaaaaaataa gaattacata taaaatatag agcttatatt cttaataata ggtgttgtaa
     9061 tattaataaa atgaataata attttaaaat aaatattcag ttacttatgt gtaattggta
     9121 taaaaatatt gagaaatttt aaaaacaatg gtaaagtgta agtataatca cgaatatttt
     9181 ttataaagta atggagggat gttcgcttga gaaaaataaa atctgaacaa atcgtcgagc
     9241 aggtaaaaaa actttgtata gaagcgagcc tatatttagg agaagatgtt ttaagttgta
     9301 taaaagaaaa agcgaaaagc gaaaagagtg aagttggaaa aaatatattg aatatattag
     9361 ttgaaaatgc tgaaatagct aaagaaaaaa atataccaat atgccaagat actggtatgg
     9421 ctgtattttt tgttgaaata ggacaagaag ttttaattga aggagacaca cttactgatg
     9481 ctataaatga gggagtaaga caagggtatg aagaaggcta cttaagaaaa tctgttgtaa
     9541 gtccaattaa cagagtaaat acaaaagata atacacctgc tgttattcat tatgatatgg
     9601 taaaaggtga taaaattaaa atagaatttg cagctaaggg atttggaagc gaaaatatga
     9661 gtaagatgaa aatgttaaag ccatcagatg gattggaagg aattaaaaaa ttcataatag
     9721 atacagtttc agaagctgga cctaacccat gtcctcctat ggtaattgga gttggtatag
     9781 gtgggactgt ggacaaatgt gctcaaatag ctaaaaaggc actttttaga gaactaggtg
     9841 aatttaataa ggacgaaaat atagcaaaac ttgaaagtga actattaaca gctattaata
     9901 aacttggtat aggacctcaa ggcttaggtg gtactacaac agctttggga ttaaacatag
     9961 agacttttcc tacacacata gcaggtctac ctgttgttgt aaatataaac tgccatgcat
    10021 caagacataa gaaggtagta atttaggagg aaaataaaat gataaaaata actacacctg
    10081 taaatgaaat tgatatagca aaattaaatt gtggagatac aattagtttg agtggtatac
    10141 tttatacagc aagagatgct gctcataaaa gattgataga ttgtataaat aaaggtgaag
    10201 aattaccatt tgacgtatat ggtcaaggaa tatattatgt aggacctact cctacaaagc
    10261 caggagaagt tataggtgct gcaggtccaa cgacgagtta tagaatggat gatttgacta
    10321 tacctctttt agaaagaggt cttagattga tgataggcaa gggaaaaaga agtgatgagg
    10381 ttatagaagg tatgcaaaaa tacggagcag tttatcttgc tgcaatagga ggagctggag
    10441 catatatttc aaattcaata aaatcatgtg aaattattgc atatgaagat ttaggagctg
    10501 aagctataag aaagattgta gtagaagacc ttaagcttac agtagcaata gatagctatg
    10561 gaaataatat atatgagcaa ggtcgagcaa tttatgaatg taaataatgt ttagaaataa
    10621 atatgaatat caaaattaaa aatagttgtg tatattttaa ggaggaaaat tgatgtctaa
    10681 ggattatgca aaattggcat tagaaatgca tgaaatcaac aaagggaaag taagtgtaga
    10741 aagcaaggta gaaattaaaa ctaaggatga tttatcaaca gcatatacac caggggttgc
    10801 tgaaccttgt ttaaaaatac atgaaaatca agatgatgtt tatagataca cttctaaagg
    10861 gaatctagta gcagtagtta gtgatggaag tgctgtactt ggattaggta acattggtgc
    10921 tgaagcatct atacctgtta tggaaggtaa agctatatta tttaaacaat ttgcagatgt
    10981 agacgctttt ccaatatgtt taaagactaa tgatgtagat gagatagtta aatctgtaga
    11041 gttgatggag cctgtatttg gaggtataaa tttggaagac atatcttctc caagatgttt
    11101 tgagatagaa gagagattaa aaaaatcttt aagtatacct gtttttcatg atgaccaaca
    11161 tgggacagca ataatagttg cagcagccat aataaactca ataaagttaa ttgaaaataa
    11221 aaaaatagaa gatttagaaa tagttataaa cggagcagga gctgctggta tagccatagc
    11281 aaagatacta cttaatatga atgttaaaaa tataatttta tgtgatagaa gtggagcatt
    11341 ggaagctagt atagaaaatc taaactatgt tcaaaaagaa atgttaaaag taacaaatat
    11401 aagaaatgaa aaaggacctt taaaagatat tataaaagga aaagatgtat ttattggtgt
    11461 ttcaggtcca ggagctgtta caaaggaaat ggttgaaagt atgtctgaga agccaataat
    11521 attggcaatg gcaaatccta ctccagagat aatgccagaa gaagcaaaat taggtggagc
    11581 atttataatg ggaacaggtc gttcagactt ccctaatcaa gtcaataatg tactggcttt
    11641 tcctggaata tttagaggtg cacttgatgt taaggctaaa gagataaatg aagaaatgaa
    11701 gatagctgct gcatatgcaa ttgctaatac aataagtgat gaagaaatat gcccagagta
    11761 tatattacca gatgtattta ataagaatgt agtaaaaaat gttgctaagg cagttaaaga
    11821 agcagcgata aaaactaaag taaatagaat ataaattttt atatgttcat atatccttat
    11881 attatcgggt atatgggcat ttttattata ggtttatact catatatcct tacttttata
    11941 tacatatctt tatgattaaa ggtgttagaa aatgtagtaa attttatact atcagactct
    12001 atctttatta ctttagtaga tacatggttg taaaggttta tagaaaaaaa tataggaagg
    12061 cagattttta ttgttgcgta tatatgagaa tgatataata tgaatattat aaattaataa
    12121 atttaaattt atggaggtac aagagatgat ggaatataaa gttaatgacc aagaacttaa
    12181 tgcttcaatg tttatcccat tcgtcaacag gatatggcca ggagattatg atatggataa
    12241 aacacagact gcattatcaa aaactttgaa tataactgct tatgatgata aagtgcttgt
    12301 aggatgtctg cgagttcttt cagatggtta ttattttgga acaattacag aattgcttgt
    12361 tcttccagag tatcagaaac aaggcattgg tagtaagctt ttccagcttg ctaaagagaa
    12421 caccccaact atgctatatt ttggtgcaca gccaggagta gaagcgttct atgaaaaaaa
    12481 tgggtgccaa agaagtctgc aatcctacat aataaaaaag aaagatagtt attaaagttc
    12541 cagctttcaa taatcttaaa agtacaatat tttagtggaa tcatttcatt ttttattact
    12601 aatatgacat aaacatgtca tattagtatt gatatacttt taatatggtt aagagccaaa
    12661 gaaaaatata aggagtgatt taaaatggaa gaaactaaat tttgccaatg ctgtgcaatg
    12721 ccaatgggac aaactgatga attatatggg acaaataaag atggaagtaa aagtactgat
    12781 tattgtagtt attgttttaa agatggtgag tttacagctg atataagtat ggaagagatg
    12841 atagaagttt gtatccctca tatgcttcaa agtaataagg atatgactga agatgaagca
    12901 agaacaatga tgaacaattt ctttccaaca ttaaaacgct ggaaataatt ttatgcaaaa
    12961 ttagttatat gcaaaacatg aaatatggtg ttgagaataa ggggtggtgt gctttatagc
    13021 ataccacctg ttttgttact tggagttcag ttattttcct aaatatttta aatatactat
    13081 tttagataag ttccttcttc atcttgataa attaatcctt ttttcataag cactccaaga
    13141 gctcgtttaa aatttttctt acttgaatta aataatatag aaatatcttt agggtcagat
    13201 ttatcattaa atctcatata tccatcagaa ccttccaaat aacttaaaat cttattttct
    13261 aaagtatcaa gttcatcctt tctatttcct ctaggagaaa gaccaagttt tccatcttca
    13321 tatattttta tgacatgtaa attttcaaga acatcaccag tttttagttc agtaaagtac
    13381 tcattatgaa gtattacacc agcatattta ttgtctacac atatcattgc agaattatta
    13441 gtttggaatc catatacaac tccagttaca atatctccta cattgtaagt atggtctgtt
    13501 aataagtatg aatctatatc tgtagtagca gcaattcttt tgcttttgtc taaatatata
    13561 taaaataaat atttttcgtc tctaaatagt ggataagtct tagctttaaa aggaactaat
    13621 atgtctttag gaagtcccat atctataaaa gtaccaatgt ctgtattatc aactactttt
    13681 aaatgtgcta catccccaac cttagctaaa ggcggtatta aagttgcagc agttctgtca
    13741 tcagaatctt taaatatgaa agcatttact tcatcaccta tgtttatttc attttttccg
    13801 attaatctat tatgaagaag tatatcgtct tttgtgttat tagtttgtcc atccaaaaaa
    13861 tatccaaatt cagtttttct ttttactgta agtttattaa aatcacctat gtttatcaaa
    13921 ttaaaatcat cctctcattt tatatttata atttagccat aatatactta tgtagtagtt
    13981 tgtcacagta tctatattag tattagtgaa atttacaata aataatatag ttatttatgt
    14041 atttttcaat tctctagctt cttatatagt ataaactcca atttcttttt tgtctttagg
    14101 ttttattaaa atttttacaa aataagttaa aaagtattga aataaaattt cttttaaagt
    14161 ataattggta tataccaaaa ttggtatata ccatatcgaa aagtggtgac tatatgaaaa
    14221 tagtaaataa atctagtaat ataccattgc atactcaatt aagttcaata ataagagaga
    14281 tgatagaaac tggtgagctt aaagagggtg atgcaataat gccagaaaga gaactttgca
    14341 acatacaaaa tgtaagtcgt atgacagtaa ataaaaccat tgttggatta gttacagaag
    14401 gacttcttta tagggtacaa ggtaaaggga cattcgttgc aaagcaaaaa aagaagtatc
    14461 agttttcaaa tgtaaaaggt tttactgatg ttatgaaaga aaagggagta aacataaaaa
    14521 cggatatatt atcctttgag atggaactac cagatgattt agttaaaaga aagcttggaa
    14581 taagtgataa tactacaaat atatataaga ttgtcagact tagatatact gatggagaac
    14641 catttggact agaaatagta tatctatcag aagaaatgtg taagggtcta acaaaaggga
    14701 tattggataa ttcttcatta tatagggttt taaatgaaaa atatggctat aagatacaaa
    14761 aagctgaaca ggtaatggag ccagtaatac tttcagatga agaaagtaag cttcttgaaa
    14821 cagatgaagg tgctttagct cttaaacttc atagaaattc ctataataga gaaggtagcc
    14881 ctatagaata taccatatca atatttagaa cagataaata tcaatatgaa atagtattga
    14941 gtgagtaggt gaataatatg aaatgtttaa ttaatggaaa gataatatta aaaaatcaaa
    15001 tcttagaaaa taaagtatta gtatttgatg aaaaaataat tgatatagct gatagtgtac
    15061 caaaagattg tgaagttata gatgctgatg gaaaatatat atctccaggg cttattgata
    15121 ttcatataca tggaaacatg ggaaaagata caatggattc gactgatgaa tctattgaaa
    15181 caatttcaaa gtcaattatg agacatggag taacatcttt tctgccaact acaatgacta
    15241 tggataaaga acatgtttat gatgcatttg aagttattaa aaaagcccaa aatagaaagc
    15301 ttgaaggggc acaagtatta ggagcacatt tagaaggacc ttttataaat gagaattata
    15361 agggagcaca aaatgaaaaa tttataataa attcaaaata cgaatttata aaagaatata
    15421 aagatgttat aaaagttata acttatgctc cagaaaaaga tattgatttt gattttacta
    15481 gagaaataaa aagatgtaca gatatagttt tatctattgg tcatagtaat gcaaattatg
    15541 accaagctaa agaagctata aatttaggag taactaatgt aactcacatg ttcaatgcaa
    15601 tgactggttt aaaccataga gacccaggcg ttgttggtgc agcacttaca actaatgtgt
    15661 acagtgagtt aatagcagat acaatccata taaataaaga tttatttcaa tttatactaa
    15721 ataataaagg aaaagaaaga ctgattttaa taacagattc tatagaagct ggtggtcttg
    15781 aagatggcaa ttattctctt ggaggtcaga aagtcatagt taagggtaat gaagctagac
    15841 ttgaaaatgg agcattagct ggaagtgtgt tgtctttaaa taaaatggta tttaacttct
    15901 tagataatac aaaccttaaa gtgaatgaag caattaattt agcttcttta aatccagcaa
    15961 catctttggg aataaatgac aaaaaaggaa gcttagaaat aggtaaggat gcagatatag
    16021 ctgtatttga tgagaattta gattgtaaaa tgacactttg cttgggggaa gtagtatata
    16081 aaaatatata gtatacaaaa caaactttaa aaacagtaaa aacaatataa aaaataataa
    16141 attaaactta cattagttaa agtagtagtt aaaatttagg aggaataaga tgagagtatt
    16201 agtgtgtaaa gattatgatg gaatgagtaa aaaagccgct gaaatgatag cagctcaaat
    16261 agttttaaaa ccaaattcaa tactgggact agctacagga agtacaccag taggtatgta
    16321 tagagattta gtaaaaaaat acaatgataa tatagtagat ttttcagatg tgatgagctt
    16381 taatttagat gaatattata agttaccaat atctaatgac caaagctatg attattttat
    16441 gaaagaaaat ctatttaatc atgtaaacat aaagccagaa aatactcatc taccaaatgg
    16501 aatggctgat gatattgaaa aagagtgtat gaactatgaa gcttctatag atgcagctgg
    16561 tggtatagat gtacaagtac ttggaatagg tcgtaatgct catataggtt ttaatgaacc
    16621 tgatactaaa tttgcaaaaa gaactcatgt agttgaactt acagagtcta caatagaagc
    16681 taatgcaaga ttctttaaat caagagaaga tgttcctaaa aaagctgtaa gtatgggaat
    16741 aggttccata ttgaagtcta agaaaatatt gcttttagct tcaggagaag aaaaagctga
    16801 tgcagtatat aatactgttt atggagatat aactccagaa gttcctggtt ctatacttca
    16861 acttcataaa gacactatag ttatagtaga tgaagcagca gcaagtaaat tgaatccaaa
    16921 agattataaa ttagtataag aatagaatgt agtttaagta aaaaatagct atcattgagt
    16981 aatcaaattt atttaatgat agctattttt atttaaattt tttacttctt tttatttaaa
    17041 ttagttatta ttgtttaaat tacttgctta ttacttttta cgaaaactcg ttatcaagaa
    17101 gtatccaaat aagatgttaa aaattattat gtttagtggg attctatcaa gtataataaa
    17161 attacctata attgggatat taccaactgg aaacataaag aagaatacag gcatcagtga
    17221 tagtaaaaaa gatggaaaac aaagaagaag taatcttgga atatcaatat accaactacc
    17281 atcctttttg tattctctaa tcaataattc agttcctagt aataaaccaa taccaccaga
    17341 ggaaattaaa ttaataaaaa tgtatctttt aaagtcttgc atataaccag tgtgaaattg
    17401 agtatagaaa tcataattta atttaacagc taaaaaatac ataagcaata gaatgattaa
    17461 aaataaaaga tatctaagag cttttttcat tttaaattct cccttcagaa atactttatt
    17521 tagttttatt ttgttggaat ctgattttat catttagttt aagaaatcca taatattagt
    17581 gaagaatgaa ataagtatta tatatgaata aatattttta gaattaaggt gaattgtagt
    17641 gcttaatata actataatat taattgtttg aatattcaat aaaaagtttg aaaaatatca
    17701 attttgatat aaactgaata aat
//
LOCUS       DAELJO010000067        17277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELJO010000067 DAELJO010000000
VERSION     DAELJO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.067"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            39..785
                     /locus_tag="KPB88_001924"
     CDS             39..785
                     /locus_tag="KPB88_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG0054204.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLINSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            846..1520
                     /locus_tag="KPB88_001925"
     CDS             846..1520
                     /locus_tag="KPB88_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0054205.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTHGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1889..3235
                     /locus_tag="KPB88_001926"
     CDS             1889..3235
                     /locus_tag="KPB88_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0054206.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEKINKLHGGIWEQIDAKVLAGESMYYYKKIKD"
     gene            3262..4656
                     /locus_tag="KPB88_001927"
     CDS             3262..4656
                     /locus_tag="KPB88_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0054207.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGEKI
                     EFGITVHYYKLIEK"
     gene            4866..6293
                     /locus_tag="KPB88_001928"
     CDS             4866..6293
                     /locus_tag="KPB88_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0054208.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     ILYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPEKTN"
     gene            6646..7590
                     /locus_tag="KPB88_001929"
     CDS             6646..7590
                     /locus_tag="KPB88_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG0054209.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISEIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEGNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7759..8691
                     /locus_tag="KPB88_001930"
     CDS             7759..8691
                     /locus_tag="KPB88_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG0054210.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8796..9263
                     /locus_tag="KPB88_001931"
     CDS             8796..9263
                     /locus_tag="KPB88_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG0054211.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9507..10361)
                     /locus_tag="KPB88_001932"
     CDS             complement(9507..10361)
                     /locus_tag="KPB88_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG0054212.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10527..12491
                     /locus_tag="KPB88_001933"
     CDS             10527..12491
                     /locus_tag="KPB88_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0054213.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDNNELKNTSTNPCETNTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12491..12952
                     /locus_tag="KPB88_001934"
     CDS             12491..12952
                     /locus_tag="KPB88_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054214.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12955..13275
                     /locus_tag="KPB88_001935"
     CDS             12955..13275
                     /locus_tag="KPB88_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0054215.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13291..14391
                     /locus_tag="KPB88_001936"
     CDS             13291..14391
                     /locus_tag="KPB88_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0054216.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14511..17111
                     /locus_tag="KPB88_001937"
     CDS             14511..17111
                     /locus_tag="KPB88_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG0054217.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRVDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6853 a         1786 c         3003 g         5635 t
ORIGIN      
        1 tttatgaaaa atgtaaactg tgaaatggag gtagtaacat gaaaattgaa aatctttcta
       61 ttgatgatat taaacgaggc tatatattta atgatgattt aaattgttat agttgcatta
      121 aatgtacaaa aacatatgag gtaggagaaa tatataaaat aggtaataga ttctttgaag
      181 cttcaaaagc tattgaactt catgtggaat tagaacatag tgattattta gagcaattga
      241 ttaatagtga ttcaaaatac aacacattga ctgaaaatca aaaacaatta tttcaattat
      301 ttattagtga tttaccagat aaggagatag caaaacaggt tggagtatcc acatctacta
      361 tacgccatca aaaatttatt ttcagagaaa aagcaaaaca ggctaagttc tatttagcac
      421 tatatgaatc tgtatttgaa gataaatcta ataaaagtag tactataata ccaatccatg
      481 aacatgctcg aatggtagat gaaagatatg tagtaactga agaagagaga aaacacattt
      541 tggatacatc atttgaaagt atgaatcctt taaaattaaa agtattttct tcaaaagaaa
      601 agaaaaaggt cgtaatttta tctaagattt tggaacaatt tgaacgtggg aagaaatatt
      661 cagaaaagga agttaatcag attttagaat ctatatatga tgattttgca acaattagaa
      721 gatatctaat tgagtatggt tttatgagta gaaataaaga atgtacaaag tattggttaa
      781 cataattaaa ataataaatt ttactaatgt gtaatgttat ttttatctag ggggaggagt
      841 caaatatgaa tgatgaatta aaatctattt tatacaataa aggtgtggac attgttcgct
      901 ttgttgatat atctgaattt ccaataaatc aaacgcacgg tttttctaaa gcaattttat
      961 tttgtatagg gttatcaaaa aaatttatta cagatatata caataactta cctacagata
     1021 gtgatgagtt tttggaaaaa gaggagaaag tagaagaatt agctgactgg atttcaaaat
     1081 atattcaaaa taaaggatat agagcgtatt ctcaatcgga gaaaaacaat ctagaacatg
     1141 gatattttga aaaagcctat attaatccag aaatgcaatc agggattagt cctttaccac
     1201 ataagacaat agcaaatatt agtggcattg gatttatggg aaaaaataat ttgtttgtaa
     1261 cagaggaata tggatgtgct tttagcatgt gtacagtact tacagatgct ccaatttctg
     1321 ttgaaagata tcctttgatt gattcaaaat gtatggattg taatgtttgt gtagaaaact
     1381 gccctgcaaa agctatacat ggaaatgagt ggactttgcc aggaaaaaga gagtctatta
     1441 ttgatgtttc aaagtgtttt tgtgtattga ggtgtatgat gagttgccca tggtcattaa
     1501 gatatgcaaa tcaaaaataa ctttactaga gaaggtatta aattgaatat tataaaattt
     1561 ttaaaatgta agattatata agatttctca aaataaaaat ttgtatagaa cactcagaaa
     1621 tgagtgtttt tttattggaa ttaaattata ttgatacaaa gcttaactat tttaatcatt
     1681 atataaaatt tgtattaaaa aattaagaat aacttggtaa tagtgaaaat ataatgagat
     1741 tataattgat ttataatata actataatga tattgtaaaa atgaaaattg tcctctcaaa
     1801 attcccaaag agagtaaaag tcacttcagt tatagtcaaa aaaatttttc tgtgaaaaga
     1861 atttagattt gaaaaggagg aattaatcat ggaaattgaa actaaaactc cagattggat
     1921 taatgtacta gaattaaaca gtaaaataga tattactggg aaatatgatg taagtaatat
     1981 gatacaaaat attgtctctg ataagtcact agaaggttcc attatatatt ttccaaaagg
     2041 taattactta tttgagaaag gtattaaaat tagtcaacaa ataacacttc aaggagattc
     2101 ttattatgga ggaggtaatc aagtatcaaa tttagataaa aaacaatcaa tcataggaac
     2161 aaccaatttt ataacccgag gagtttctaa tatgagtatt attacattgt caggaacaag
     2221 tcaatgtatc aaaaatatta atttttatta tgatagtcat gatatagaga aaataccgcc
     2281 taaaaatgta tctgctatta cagaatatgg agaaacacaa ggactatctc attttgaaca
     2341 tctgtttatt tctggatttt cagggatagg gattgaaatt ccatactatt caactggtaa
     2401 tgatattact gttagttcat gtggtcttgg tatgaggctt ggagagaagt caatgctttc
     2461 tagtagcaag atttacgaat gtaaaaatgg aatggagatt actactggtg ttagcttaaa
     2521 taatgtgaga atagaagaaa taaaagaaat aggtattaat aataaaggat ttggttttaa
     2581 cctgattatg aatcttatgg tcgaccaatg tggatattgt ggatttatgt ttgaaaaaat
     2641 gtgctatagc cagataactg ccagaattac tagatgcggt cagtattata aaagtgttga
     2701 ttataatgct tatgagaata tggaagatag gaaagaagaa gcttattcca ttttttatgg
     2761 agatatttta gaaaattgca atattgtatt gatgaatagt aatattgata ccttggaaaa
     2821 tgattcactt aatgaacata agatatatgt aatcaaggca aatgaaacaa acaatgtaat
     2881 gctaacatgt tatgctgaag cagataactt tatacaatgt gcaaaaggta acttgctgtt
     2941 agaaaatggt gaaaatactt ataaatttta taatggtgag atttgttctg taggaggtgt
     3001 tcaaatttct gatatagatg aaaaagattt aatcaaaata atcgagaaca ctctgtatat
     3061 agatgataaa ggtaagtatt taatggttcc tagattaaaa aagaatactg taatatattc
     3121 agtactagat tctacagaaa aaataaataa attacatggt ggaatatggg aacagattga
     3181 tgcaaaagta ttagctgggg aatccatgta ctattataaa aagataaaag attaaattat
     3241 caaactagga ggtagtgatt aatggaagag tctataataa ttaaagatat aagtggctgg
     3301 attaatgttc ttgattatgg aatttgtaat gatgaaacaa aagatgtaag ttccaagttg
     3361 caagagatta tggatgctgt accagaagat tctattatat attttccaaa aggaaaatac
     3421 ttatttgaaa gtggtgttaa gattaagaaa cgattaactt tatgtggaga ttcttatatt
     3481 ttggcgaatg ttcctaaaaa tactgctgga gtaacacaat ttacttttaa aggaaagaaa
     3541 aatcctaaaa gtgaaaatat agcaattata acagcaaaaa gtgtaagaca ctgtattaag
     3601 agcatttgct tttattcaga tagttgtgat agagatatga catctaatca tgaacctcca
     3661 acagatggaa aacctagatt tcatcataat gtaattataa attatagaaa tatatcggct
     3721 attgtatgtg aagataaaca gggagaatca ggtcattatg aaaatctatc ttttagtggt
     3781 ttttctggaa ttgcacttga tatgcctaat aattctacag caaatgatat tacaattttt
     3841 acatccggac ttggaattag tacaggagaa aattcaacta ttgctaacag taaggtttgg
     3901 ggctgtgttg atggaatgat aatttctact ggaactttta taaatggtat gagggtagaa
     3961 gaaataaaaa aagttggaat taaaaatatt ggtagaggtt taaattttat tacaaatatc
     4021 acaattgacc aatgtggata ttgtggagtt tggtttgatt ctatatccaa tgttcagata
     4081 tcaggaaata tcacaaggtg tgggcagtac tattatgatg taagctatga taattattta
     4141 atgttggata atagagttaa agaagcatat tctttattct atggtaattt attggaatca
     4201 agtaatatag ttttatcaaa taccaataaa gataattggg aagatttcaa agaagataag
     4261 cataaggtgt atcttgtaga agcacttgaa accaagaatg tactattaac atgcaatata
     4321 gatgctactg actggattgt taagtcagaa aaaggaaatt taacgtacaa taatatgcga
     4381 caaactgtta tattttacga tggaaaacta agtagtatca atggaattgg tatatctgat
     4441 gaagattatg gggataaagt caagataaaa aaaggaaata tatatataaa tagtaaggat
     4501 attaagatgc ataaaccaga ggtaggtgac attatgtcaa caattattaa agataaaagt
     4561 attctttctg ctcattatgg gggaacatgg gagaaaattg gtgaaaaaat agagtttgga
     4621 ataactgtac actattataa attgatagag aaataagtaa atacgtagta tttgaataga
     4681 tgtattttat tggagagcta ggactatagt tttaatgtaa atggtttggt aatagcatgg
     4741 attgaataat ataaaaataa tataaaaaat taatttgatt atgtaaaatt ctatgaagta
     4801 gtaaaactgt gactttagtc ataggaggtt caaactaagt aaaagggaaa ggaatatata
     4861 atattatgga aggaagaact gggattaaag atggaagatg gattaatgta gtagaaagag
     4921 gtattaaaaa tgatggttcg tacaacgtag gtcctgcttt gcaggatatt attgatgaaa
     4981 tagaaacaga tttttgtatc ttatattttc caaaaggtaa atacctattt acaagtggtg
     5041 ttaaaattaa taaagaaatt gtattgcaag gtgattctaa ttcatctaca ggcactacac
     5101 aatttataac tcgtggagta gaaaatatga gtattattac attgacaggg aaaaaacaat
     5161 gtattaaaaa tataaatttt tattctgata gttgtgagat acaggtaaat aaagaaccac
     5221 cgacaaaagg taatcctaaa tatcattatg agatgattat taataagaat gaaaagaatg
     5281 ttgagatgaa taatgtatca gcaatcctgt atgataacag taagaatatg attaaaggtc
     5341 taggtcatta tgaaaatatt tatataagtg gtttttcggg tacaggaatt aggataccat
     5401 attactcaat tgtaaatgat attacagttt catcttgtgg gctgggtatt gatacaggaa
     5461 tagacacaat tatttctaat agtagaatat ctaagtgtca aaatggaatg aggattagga
     5521 ctggaactag cataaataat gtaagaattg aaaaaataca gaaagttggt cttataagta
     5581 gtcaagttaa ggggtatgag gggtatggaa gttataaaat taataatatt acgattgacc
     5641 aatgtgggta ttgtggcttt tcatttgatt caatgaaaga tagttatttt tctggtatta
     5701 taagaaggtg tggtcagtat tattataata cagattatga cacatattta agcattaaag
     5761 atagagtaga agaagcatat tctttatttt atggaaatta ttttacagaa tgtaatattg
     5821 atttagttag tgataataag gataactggg atgatggctt agaatataaa cataaagttt
     5881 atgtatttaa aacccttcaa ataccaaaat taacattgag atgcaatata gatgcctatg
     5941 attatatcgt taaatcggta catggaggag gtaatttaat gttaaaaaat aactttaaca
     6001 attatatatt tattgattca tctagtccag aagttattaa tggaattaat tttaattatc
     6061 aaaagaatgg aaacctgtta aggttaaata atggtaaatt aagttataaa aatggaaagg
     6121 aagaaacagc tatattcaga cctaattatg gtcttattgt agcatcaaca gattcagaat
     6181 gtgaacaaat agagactagc tatggaggaa aatgggagaa gcttggttct gaagtaattg
     6241 gagaagaaac ggtatattat tataaacatc atgaaccaga aaaaacaaac taaatacaat
     6301 aaaatttgta ttatataatg atataaagtt ttaatatata gtgtttacct ttaatgtaat
     6361 attggtatat ttgtatagta ttaaatttgt taaaaagtaa aattgtaggg tgcttgaaaa
     6421 tagcaccttt tttagtttca ttataaaatt agttttgaaa gtttaggaat aatgtatatt
     6481 tatttttaaa gaattataat ctcattaagg atattttaaa ttctagaaat gaatcagtaa
     6541 atcatactgg aattatctta aatgttttga ttaaattaag tatttagagc tataattata
     6601 ttaaaaatat attagaacat aattatatta ctatggaggt taaatatgat agtaagtgtg
     6661 agtagaagaa ctgatatacc tgctttctat agtaagtggt tttttaacag acttgaagaa
     6721 ggttttgttt atgtaattaa tccaatgaat ccaaaacagg ttagtaagat agagttaaat
     6781 ccacatactg ttgattgttt tgtattttgg acaaaggatg taactccaat gttatatgat
     6841 ttaaataaat taaaagacta taaatattat tttcactata ctataacatc atatggaaaa
     6901 gaagtagaga catgtatttt agataaaaga aaggtaatag attcatttaa ggaattgtct
     6961 agaaaaatag gcaaagaaag agtaatttta agatacgacc cgattttctt aagtgaaaag
     7021 tacacagtag attatcatat caaagcattt gcgagtttat gtaatcagct tgatggattt
     7081 acagaaaaat gtataataag ttttatcgat ttgtataaga aaacaaaatt taatactaaa
     7141 gcattacata taaagccaat agaaattaca gaaatagaaa cattatcaaa agagatttct
     7201 aagattgctc ataagcatgg gataacactt gaaatgtgtt ctgaagaata taatttaagt
     7261 aaatttggga ttggaaaaag taagtgtatt gatgatagat taatatcaga aattatagga
     7321 tgtgaagtag aggtaaaaaa agatagtaca caaagagata tttgtggatg tgttaaaagt
     7381 gtcgatatag gacaatacaa tacttgcaga cattattgtc tgtattgtta tgctaacttt
     7441 aattatgcaa aagtagaaga aaactgtagg ctttataaag aaggtaataa attactcata
     7501 gatactttaa gagatgatgc aaaaataagc attagagata tgaagactat taaagtagat
     7561 aagaataaac aaattagtat atttgattaa aataaatata ctaattacct taatatatta
     7621 agaatataga tacttttgat aacaaaatta aacttaatag atattttcta tataagaaag
     7681 atggtcataa tatgtcaatt tgaccatctt tttgatataa ttaaaacaca aataatttag
     7741 taacaaagga ggcagaactt gaatttatta aaaaaatatc ctatacctat tgcaagtttg
     7801 attttgtcaa tctttacttt aggtaactta ttacaatctt atagtgaatc attacgtaat
     7861 gtaattggat ttataggatt tattttgtat gcaatttata tagtcaaaat aatattactt
     7921 agaaagggtg ttaaagagca attagagaac cctttaatat caagtacatt tccaactata
     7981 acaatggcaa caatgatatt tgctacatat ataaaaccat tatctttaga attaagtatt
     8041 atgatttgga gcataggaat tgtgggtcat cttgtgttaa tagtcttatt tagtaagaag
     8101 ttcttagtta aattctcaat aaaaacggta tttccatctt ggtatatagt atatgttggg
     8161 atagttgcag ctagtgttac ggcaccagca tttaatcaac ttttgattgg caaaattgct
     8221 ttttggatag gatttgtatc ttatatagtt cttataccag taattttaaa gagagtatgg
     8281 ataatcaagg aaatgcctga accatcttta ccaagtatag ctatattagc tgcaccaggg
     8341 tcactattat tgtctggata tattaattct ttttcagata aaaataatgt agtattatac
     8401 ttgttattca tattatcaat tgtattttat attatagttt tgggatattt accgaaatta
     8461 ttaagattgc ctttttatcc gagttttgct gcatttacat tccctatggc aataagtgca
     8521 atgggtatga aactaatgaa tggttattta actaaatcag gtcattttat aagttggatt
     8581 tcatatgctt ctaagttaga agagataatt gctacagtga ttattttata tgttttagtt
     8641 ttgtttatta tatatcttag taaatctgaa actacatcta aagctaaata agaattatta
     8701 aatatgtgta tttaatagga atattgatat aaatatatga agataagata aaccatagaa
     8761 acttaattta gcttaatttt attggaggaa aacaaatgaa aacaaaagaa gaatatttaa
     8821 aagaagttag aaacatatca gaagaatact ttagaaaagg agagtttttt tgctctgaag
     8881 cagttctcca gacaataaat gatgctttag gccaaccttt aagtccagag ataactaagt
     8941 tagcatcagg atttccgata ggacttggaa aggcacaatg tctttgtgga gcaatatctg
     9001 ggggagagat ggctttaggt atagtatatg gaagagttca tggtgaagct atgaatccaa
     9061 agatgtttga gcatgctaaa ggtttgcatg attatattaa aaaagagtat ggtgcaacat
     9121 gttgtagagc tattacaaag aaatgggatg gtgataactt tatgtctccc gaaagaaaag
     9181 agcactgtat taaaataaca ggtcaagtta cagagtgggt tgcagataag cttatagaag
     9241 atgaaaaact taatatcaaa tagtgtaata ttagttggtg aatttataaa gttaaattta
     9301 cttatagtat aagatacttg atattctcgt attgatagaa aaatttatag catatgtatt
     9361 taatgaaata tacttatatt tagagtgtgg tttaatttca taaatacata tgctattatt
     9421 ttaagtatat aagcaaattt atttaaataa atagaaacag ttaatttata tgtacaaaac
     9481 tatatatgat tagtgtaaat aaatacctat tttataatac tcaacataaa gacaccagac
     9541 atgattagta ttataccaat aaaaatagca agccattctt ttttgttctt cttttcttta
     9601 aaaaatataa tccctcctaa agtggctact atcacacaac tctgagagat agaaaaactt
     9661 attgtaactc ccaatgtatt agtcgttatg aacataccta aatttgctat tgaccacaat
     9721 ataccagtaa ttaaattgaa taaaacattt tccctatata caatctcttt agaaaaatac
     9781 ttgacaataa tagatgaagt tatcaacata ccaacagatt gaggtaatat aatcctatat
     9841 ccttcaatta aaaatatttg attagttaca acatataatg ataaaaatag agatgataat
     9901 aatacaactc caacacattt taacatattt ttatctgtag attttttagt ttgataattt
     9961 gtgagaagta taccaagtat tatacataat attgataaag aaccaatcag taacatttta
    10021 gaactggtcc attcattaaa caagattaca gctaataatg tcgtaaaagt taattgtgaa
    10081 ccatttgaaa ttggcattac tttagataca ctagcaattt gaattgcttt aaactgcatt
    10141 aattgtccca aactccagaa tatgccagaa atcatactta tacaaaatga aaaaaatgta
    10201 atttcaggat gtataaccag agttattata gtggaaaata taaacgcact tatggatgta
    10261 cctaaaagct gttcatgagg agaacttttc tttagatttg caactatggg catcaatccc
    10321 catcccatta caggaagtaa agcaataatt attgtcagca ttaaactatt tcctttcata
    10381 ttaaatttag acagctttta atttatcatc taaaattaaa gtgttcaata cataaaaatt
    10441 accgtattta atcaggaaat attagtagtt ttttagaaaa acattttcca ttaaaataaa
    10501 gacaaaggga aggagtaaaa ttgaaaatgt taactgatat gattcaaaat cgtgaaatta
    10561 aaataataaa gtttttatta aaagaaggtc atacaactgt aaaagcaata gcagatgatt
    10621 tagatgtttc agaaaaaaca gtttcaaaat ctcttaaaga agtagaagca gtgcttaaaa
    10681 agataggtct ttctttaata agaaaaccta agataggtgt ttatattgat ggagatttaa
    10741 aaaaaatact tccttaccta gataataaag gaagtcaaat tcctacaacc agagaggaga
    10801 gagttatcta tatatattca gtacttttaa agagtaatga ttatattact atacaacaac
    10861 tttctgatga actatatata agtcgtggaa ctattgaaag ggatttagca gaagttgaaa
    10921 aactgctaaa aaacgaaggg atttccatat ataagaaacc tagtaaggga ataaagttaa
    10981 atataagtga aagagataaa agacaactta ctgctaagtt tatacataaa ttttggagta
    11041 gaaactggta tattaagcaa gagcaagaaa gcttttatca agcatttgaa aagatacagg
    11101 cagatgtaaa tggtattttt tcggaagatg atttatcaat aattattgat attcttagga
    11161 atttcagcag agaaagaaaa tttgagttct ctgattatga atttcagtca ttagttattc
    11221 atttggcaat agctgtagag agaattagaa aaggcgaata tatagaagaa agtttgtata
    11281 ttggtaatat agaaaataat caaatgtcaa ataccaatta tttagcaaaa caattagaag
    11341 aaaaattttc aatattatta ccaaaatctg aaattggata tataaattta catttagtag
    11401 cgtctgacca acatagagat aataatgaac taaaaaatac ttcaaccaat ccttgtgaaa
    11461 caaatacaaa taatatatca aatcttcggg aaattataaa aaatacactg tatgagacag
    11521 cttatgacaa ggatttaatt gatggattga cttcacatgt acattcatca ataaatagac
    11581 tgaaatatgg acttagtatt aaaaacccat atgtaaatac aatcaaacaa aacttttctt
    11641 tatcatttga gcaagcttta gatttaaaga aagtaattga atttatatat gatattagga
    11701 taaatgatga tgaatgtgct tatatagcat tacattttga agcttttaga gaaagaatga
    11761 aagaacttcc acaaaatatt agagtaattc tagtatgcag tacaggatta ggttcatcta
    11821 ggttattgtc tgcaagaata aaaaaatact ttccaatgat aaagattgaa aaagtacttt
    11881 ccgtacaaga attagtatcc agtaaaattg atgcagattt agtaataagt actatttatt
    11941 tagagttaga aacaattcca accatagtag ttagtcctat tttaaatgaa agtgatataa
    12001 gaaccttgga aagaaatata caaaatgttt caagttgtac ttctaagaag tataaatctt
    12061 tcagaaattt aatctttgaa gaaaatatat tgttgaatat tgaagcttca aattatgaag
    12121 atgcaattaa tcatataaca gataacctaa taaaaaaagg ttttgcaaaa gaaggtatag
    12181 ctaaaagtgc aattgagaga gaaaatttat cttacacgtc tttccagtca atagctacac
    12241 ctcatgctaa tcctagttat attaaaaaat ctaatatatc tgttgcaacg ttaaaaaatc
    12301 ctattacatg gggaaatgaa aaggtggaag ttattttctt tatttcatta gaaaatgaca
    12361 aaaacctaga gcttgatgaa atatatgaag ttttttatga cttcattgac aataaaaaaa
    12421 atataaatga catattaagt gcaacttcca caaaggaagt gtacgatttg ctagtggagg
    12481 agagtatata gtgaaaatat cagatgtatt gaaaaaagag caaattgttt ttaatcttga
    12541 tacaaataca aaggaagaaa caataaaaaa attaagtagt atatttgttg aaaatgggat
    12601 tgtggacaat gaagaagaat atgttaagtc tgttttggaa agagaagaac attcaactac
    12661 aggtattggt aatggtattg ctattcctca tgggaaaagt aatgctgtca aaaaatcttc
    12721 tataatattt gcaaagacta agcataagat tgaatgggat gctttagatg aaaaaccagt
    12781 agatagtatt ttcttattgg ctataagcaa tattgataag gatagcaatc atcttgtact
    12841 attatctaaa ttagcaacta aacttatgga tgatgacaat gtagatgcac taaaattagc
    12901 caattcagaa caagaaataa tcaatatatt tagtgaagaa ggagagatgt agaaatgaaa
    12961 agaaaaatta tagcagttac agcatgtgca acaggagttg cacatacata tatggcagca
    13021 caagcattaa agaaagcttc aaaaaagatg ggtcacctta taaaagttga aacacaagga
    13081 gctacaggaa tcgataatga gttaactaca aaggatgcac aaattgcaga agtagtaata
    13141 tttgcagtag acactaaagt cagaaatgct gaacgttttg agggaaaaga aatactagaa
    13201 gtaccagtta atgcacctat aaaggatgca gagaaagtaa taaatgatgc attaaagtta
    13261 attgacacta aataaatgga gggaaatgta atgttgaaaa agatagtctc tgagttaaaa
    13321 aagcatgtct taactggaat ctcatatatg attccattag taattgcagg tgctatgata
    13381 atggcgatat ctcgtgtagg tggttctttc tataatatac cagatatatg ggatgctaaa
    13441 tatgcagaaa gtgctagtag tattgttaga ttattacatg atttagatgg gtttggaggt
    13501 actgcattag gattaatgtt tcctgtaatt gctgcattta taggattttc tatagctgat
    13561 aaattagcta ttgtaccagg tcttgttgga ggtatgattg ctaaagatat aggtgcagga
    13621 tttttaggag ctttggcagt tggtcttata gcaggatata catgtttgtt tattaaaaat
    13681 catgtcaaat taccaaaatc agcagcttct atagtaccaa tatttatagt accagtattt
    13741 gggacattaa tcactgttgt attaatcaat tatgtgatag gtataccttt tgccacatta
    13801 aatactggat tagaaaattg gttaaatgga ttatctggta caaatcagat attaatggca
    13861 gctattatag gggctatgat ggcagttgac ttaggtggac cagttaataa agcagcttta
    13921 acaacatcac ttgcattatt aacaagtgga atttatgcgc ctaacactgc tgcaatggta
    13981 ggaatagtta taccaccact aggattggga cttgctacta tactagcaaa acaaaaatat
    14041 aataaacagt tacgtgaagc tgggaaatca tctcttataa tgggtttaat tggagtaagt
    14101 gaaggagcaa ttccttttgc agtagaatct ccattaaaag taataccttc atgtgtaatt
    14161 ggaacagcaa ttgcttcagc tatggcagtt ggtttaggtt cagtaaatgc aactccaatt
    14221 agtggatttt atggatggtt tacagtagag aattggccaa tgtatgtgtt atcaatagca
    14281 gttggaacag taattgtagc tggattagtt atagtattgc gtggaaatcc agtgattgag
    14341 gatgatgaat ttgaagatga tgattttgat gaagcagagt gggaatctta aaatttaata
    14401 actatttaag acgtgttata agctatattc aatatgaatc ttatactaaa taaagtgtta
    14461 aatattcagt ttagacttaa atataaaaga taaagaacag aggagaataa atgataaaag
    14521 cacatattgt taatcataca cactgggata gagaatggta ttttacttct ggagatgcat
    14581 tagtacttag tgaacaacta tttacagatg taattgatga attagaacgt aatccagatg
    14641 taagttttgt acttgatgga caactttcta ttttggatga ttatgtacaa ttacacaaag
    14701 aaaaaataga aatcattaaa aagttaataa aagaagatag actacatatt ggaccatggt
    14761 ttactcaaac tgatgcattc tttgctagag gagagtctat tttaagaaac ttaatgattg
    14821 gaatttttga aagtaaaaag tatggtaagt atatgaaaat tgggtatttg ccagatacat
    14881 ttggatttaa tgcacagatg cctatactat taaagcatgt aggtcttgat aatatattct
    14941 tttggagagg tatacatctt ggtaaacagg ttcaatcacc atattttaag tggaaaagct
    15001 taaatggaga aagttatata tatgcagtaa atatgccaca tggatatgga actggtatgc
    15061 ttttagagcc ttcattaaag tatgttaatg gacgcttaga cccagctatt gattttatta
    15121 aatcatatac tgatgtagat gaggtgttaa taccttctgg gaatgaccag ttaaatataa
    15181 taggtgattt caaaaataaa attaaagcga ttaatgatat cggaaagtat tcatatataa
    15241 cgagttcata tcaagagttt ttaaaatata taaagtccat agaatcacta gaaagctata
    15301 gaggtgaatt tagagaacct gtattagcaa gaatacataa atcaattggt tctagtagag
    15361 tggatataaa attagcatgt gataaactag aaaacaagct gattaaaaga atagagccat
    15421 tattagtaat tgctaaaaag agtaaaattg aaataagcaa tcagttgctt ataaatacat
    15481 ggaaaaaact tttggaagga caagcacacg atagtttagc tggatgtgta actgatactg
    15541 taacagatga tattcttcac cgtataagag aagcaaatga aatatgtgac agtattgaaa
    15601 atactattgt aaaaaagatt tcagaagggc ttaaattatc taaaaatgat attttagtat
    15661 ttaatacaga agctaagcgt tttaatggat ataaagagat acaagttgta agtgatagta
    15721 aaaatattta ttttaaagat aatttagatg ctactataat tgaagaaact tatgtaaaac
    15781 cgagagaaaa tgtattagaa gaaacacctg ctggtaatat atttattgaa gaaccaggat
    15841 attatatatt aaaagtaaga ataaatatac agcttcctgc acttggttat aaagttgtta
    15901 gttttgaatt aagtgataaa gaaatggtgt cacttgataa atctaatgac acttttatat
    15961 ctaatgataa ttataagata atatatgaag atggaagctt aaatttacaa cttaaagatg
    16021 gaacttacat atcaaacttt ttaacattaa aagatagtgg taatgcaggt gatacctatg
    16081 acttttctcc actaaaaaat gatgaggata taaaactttc gtttgataaa gtgtatacag
    16141 aaaaagcttt aggatatgaa aaaatggtgg ttaaaggtac tactttacta ccacttacat
    16201 tagaagatag aaaaaataaa tatcttaatg ggaaattaaa tgtaaaagta agtattgtct
    16261 tatctaaaga aaacccattg atggatgtaa aactttgtgt tgataacacg atatataatc
    16321 atagactacg tgttcatata aaaactgata taaaagataa taaaaacata gcatcattgc
    16381 catttggata tataacaaga aaaaatggag ttttagacaa ctgggaagat atttacagtg
    16441 aaatgcctat agatttagaa cctttggaaa gtaatattac tctaacaaac caaaatagaa
    16501 gttgtactgt atttactaga ggaataaaag agtatcaaca tattgaagat gaaattgcat
    16561 tgaccttact tgctactaca gatgaactag gaaagcctga tttattatat agaccaggac
    16621 gtgcttctgg tgatacaact aaaaaaggtc atataagaat taaaacagat aaagctcaaa
    16681 tgcttaagga attagaattt tcatttgcaa tatatatgga ttctaaatca tttgatgaat
    16741 tggaaattgc gaatttaacc cacaattatc taagggaatg tgtaaattat caattacagg
    16801 attacaattt ctttttatat cgtatagata ataagattca aaaatcaatt gtcaataaaa
    16861 ctgtaagtag agaattagaa ataatatctt taccagaaaa ctatcttata tcagcatgtt
    16921 atccttctta ttacagtaaa gataaattta taataagagt ggaaaatcca agtagtaata
    16981 gaatgatatt ggatgagcaa atatttaaaa atagaaatgg gaaaattgta aatgcaatag
    17041 aagatgtaga agaggaacag gtatatgaaa tagcaccttt tgacgttatt tcaatattat
    17101 tggatttata agtgtaaaag taagtttatt gtttaaagtc tgacagatat taatctgcca
    17161 gactttttaa tttgtataaa tttgggtaaa tattaatgaa tggttttagt atttaagtat
    17221 gaatttagta tttaatatag aatttattga agtagaataa ttagtttaac aggataa
//
LOCUS       DAELJO010000068        17065 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELJO010000068 DAELJO010000000
VERSION     DAELJO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.068"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(75..2030)
                     /locus_tag="KPB88_001938"
     CDS             complement(75..2030)
                     /locus_tag="KPB88_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054218.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYENLEKRENFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(2213..3688)
                     /gene="spoIVA"
                     /locus_tag="KPB88_001939"
     CDS             complement(2213..3688)
                     /gene="spoIVA"
                     /locus_tag="KPB88_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG0054219.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3860..5155)
                     /locus_tag="KPB88_001940"
     CDS             complement(3860..5155)
                     /locus_tag="KPB88_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0054220.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5339..6748)
                     /locus_tag="KPB88_001941"
     CDS             complement(5339..6748)
                     /locus_tag="KPB88_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0054221.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPSSLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(6800..7939)
                     /locus_tag="KPB88_001942"
     CDS             complement(6800..7939)
                     /locus_tag="KPB88_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0054222.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8258..8908
                     /locus_tag="KPB88_001943"
     CDS             8258..8908
                     /locus_tag="KPB88_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054223.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(9065..10084)
                     /locus_tag="KPB88_001944"
     CDS             complement(9065..10084)
                     /locus_tag="KPB88_001944"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0054224.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(10115..10729)
                     /gene="plsY"
                     /locus_tag="KPB88_001945"
     CDS             complement(10115..10729)
                     /gene="plsY"
                     /locus_tag="KPB88_001945"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG0054225.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10764..12083)
                     /gene="der"
                     /locus_tag="KPB88_001946"
     CDS             complement(10764..12083)
                     /gene="der"
                     /locus_tag="KPB88_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG0054226.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(12117..13445)
                     /locus_tag="KPB88_001947"
     CDS             complement(12117..13445)
                     /locus_tag="KPB88_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG0054227.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13683..13883)
                     /locus_tag="KPB88_001948"
     CDS             complement(13683..13883)
                     /locus_tag="KPB88_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054228.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13885..14817)
                     /locus_tag="KPB88_001949"
     CDS             complement(13885..14817)
                     /locus_tag="KPB88_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0054229.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14862..15794)
                     /locus_tag="KPB88_001950"
     CDS             complement(14862..15794)
                     /locus_tag="KPB88_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0054230.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15849..>17065)
                     /locus_tag="KPB88_001951"
     CDS             complement(15849..>17065)
                     /locus_tag="KPB88_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0054231.1"
                     /translation="TITLRKYLKEFIYVSKKVSNNEYHARLNISAKGELGELARNFNH
                     MIKNMDKTLEEVQYKHLQLRSILKSISHGILAIDVNGNILLINDEAKEMIKSEPNQLV
                     EGHNINVVIKEEEILKQILQFIGSKDNETANITTKDDIVYKIKVDPVYLQNTNNVIIG
                     SIVNIEDITKKVKLENMRSDFVANVSHELKTPLTSISGFVETLKSNENIDVNTRNRFL
                     EIIESESDRLKRLIDDILLLSFVENKDTLLMENVVVYDVFKEVYEITQHSASEKNIEV
                     LYKCDDKSISILSNKDYIKQVFLNLVDNAIKYTQDNGIVQVVVLKDEEKIVIKVIDNG
                     VGIPKEDVERIFERFYRVDKARSRDVGGTGLGLAITKHIVKSLNGCIKVKSELGIGSE
                     FIVTILQKNFLK"
BASE COUNT         5373 a         3136 c         1844 g         6712 t
ORIGIN      
        1 aaatacagtt ttcataataa tgaaaggtat tacttagcaa tctcatgatt ttctaaataa
       61 tacctttcta aaaattattt ttttaatttt atgaatttaa ttatactttt tctagtatca
      121 ggatttacaa ttaatataga accaatcata attggtgtta gtatacaaaa taaaaaaata
      181 atatcttttt ttagattata ttctttagat tctaatttat aattattatt tattattcca
      241 tcataatcac ttatatttga cttattttta tttgtatctt gatttttgtt agaaatactt
      301 tccctataag gaaaagaatg ttcattggta ccaaatactg attttgagct taaatcataa
      361 ttatcatcat tttctgtaat tgaattttta ttgtaaaatc ctttttcttt atcagaatta
      421 ttttctttat taccgtcttt aggtatatca tgaggatttt tattttcatt gctatcagta
      481 cttgattcta catcatcttt aggagtattt ggttgtttct catcagaatt atcattttca
      541 ttattattat tttcattatt attgttttga ttgttattac tatcattatt ttcatttcca
      601 ttattcccat cttgattgtt atcatcctca tctctatccg ggtcgtctct acctcctcct
      661 ccacggtcac catcaatatc atcattttcc cgttcatcat ctggcgtctc ttggttgtct
      721 ccgcctccca ttataaatcc tggtttaaaa actatatttg aatacccttc ttttaatcct
      781 cctacaactt ttactctaaa acaacgcaac ttaaaatctt caaaaaaaag tatagcttga
      841 tctctaatat cctcctgctc ttcagatatc caattgattc catcgcttga atattcaaca
      901 aaaaccttac ttgcactata cggcatatca taaaataaaa cagctacata tccatttttt
      961 gtattttgaa attccagttc caaattgtct attggttttt tctctataac agatacattc
     1021 aaactaggaa ctacatcatc atttattata attttttctt tatttaacat ttctgtttta
     1081 aactttcctg ttatattaaa attttctctc ttttccaaat tttcataata atcagtaaaa
     1141 tcccattcta catctatatc aagtcgatta ccactagatg tttctaatac catatatttg
     1201 ggaaatttta tattttctat tttatcatct ttacttactt ttatttcatc actaaataca
     1261 ccaattcccc tatgacaaca tttcacaata tcatagccag aatcttcagc tatagagggt
     1321 ataagctcat tataatcatc aattatataa actttcttac tttctgatat aattgattgt
     1381 gccaacttat tattatctct tagtaaccca ctttcattgt tgctactata tgcttttatt
     1441 gaagtttctt ctatctcaac atcaccttta gaataaacac ctatagcatg ttctccattc
     1501 acttcaatat ccttatttag tattctaata tcattttcag tataaattcc tattgcatct
     1561 tttccatttg cctttatttt ttcaacagat tcgtaggatg atttaatatg taagtctcct
     1621 ccttcttcaa cgtataaggc tgtcccatca gtagcaatta tgttgtttat accaatactt
     1681 atacttccac cattttctat atgtattact ccttcttttc ctccttcacc tattatattc
     1741 aattcattat catcaataat aaatctccca tgatttttta tcaatatttt atatttgttg
     1801 gtatctattg taaaattact gtcataagga atataaaatc taaactcttc atctattacc
     1861 atatcacaag aaagagtata aactatttct tctttattat ttttattttc ttgaacccac
     1921 ctttcaaaat cttcaagaca attttcctct tgtccaaaaa ctttatgtgg aaaacttata
     1981 aaacacaaaa ctattatact cataaaaata aatatctcat actttttcat cttacactcc
     2041 tagaaatttt tagctttgaa acaatcctgt cgaaacatat acttcaccca ctataatttt
     2101 tccacaatta tcaattattg tcaatttata taagtatgtt tattaattgt tataaataaa
     2161 aagctactta ctaaagcaaa cagacgtttc actttaataa gtagctatat ttttataaca
     2221 aaatagttat aatattagaa cttccttcat ttacaatttt ttgtaaagtc ttttgcattt
     2281 taactcttat ctcttctggc atagtatata atttactttg taattgctct tttactaagt
     2341 catgtagtga cttaccaaac atatttgatt cccataaatc tgctggttgc tcttcaaata
     2401 cttccataag atattttatc atctcttcac cttgattttg atttccaact attggagaaa
     2461 cctctgtaga tatatctgct tttataatat gaagtgatgg tgcattggct tttaacttga
     2521 taccatattg ttttccttgt ttcattattt ctggttcttc cagactaagt tcttctaagg
     2581 aaggagctac tactccatat ccttttatct tggcatctat taaagcactt tctattttat
     2641 catactcatt tttaacttta gatagtctag ttatcagact aagcagttga taatctcctt
     2701 caattttaaa tccacttttt tcttcaagga cattatagaa taaatcttgc tttgtctgta
     2761 aatcaatatt tataacacct tctcctagct ctacattatc aacacctgta tcttccaaaa
     2821 actctaactc agaaaatcct tgtataatac cttctatatc ccttatttta cctatatcta
     2881 taatactttg ttttaatgtt gttattatgc tactttttat ccaatgattt ctttcaagac
     2941 cttcaaccca ttttggtaag ttgattctta tttctgtaag tggaaaatca tataatacag
     3001 cttccataac ctcctctata tcttcttctt ccatttcaac cacattcatt ggaagaactg
     3061 gaacttcata cttttcttcc aattcacttc taagcatact cgtttcctca ctttttggac
     3121 ttaacgtatt taaaaccact gcaaatggtt ttttaagatt tttcaattct tgtataactc
     3181 tctcttctgg ttctacatag ctttttctgt caattccagt aactgaacca tctgtcaaga
     3241 caactatccc tattgtagaa tggtctttta taactttttt agttcctatc tctgctgcct
     3301 tttcaaatgt catagcttct tgtgaccaag gagtggatac taatctttgt tttccacctt
     3361 cctcatgccc taaagctcct tcaactatgt atccaacaca atctaccatt cttactttta
     3421 aagatactgt gtcttttatt tttatttcta caccatctgc tggtacaaat tttggttcta
     3481 ctgtcattat cgttttacct gaaccacttt ggggtatctc atctcttgtt ctatcctttt
     3541 taaattcatt gtctatatta ggtataacca acttttccat aaattttctt ataaaagttg
     3601 attttcctgt tcttacaggt ccaactactc caatatatat atccccctga gtccttttgg
     3661 atatatcttc gtatatgtta ttattcatat tattcctcct atacatctaa tcttattaaa
     3721 ttatatttag agttaagggt taatattctt aaaatagaaa aataaagacc agcaatatta
     3781 atttaattta catatagaaa tttgtaaatt tcaatgtaaa ttaaattaat attgctggtc
     3841 caaatagtag tgtattatat taactgactt tagtagtatt ttcatttgaa ttattattca
     3901 tctcaataag ttctttacgt ctttcttttg tcatgttcat ccatataaat gtaaacacca
     3961 atccaattat ggctactata gtaagaacta tgaagtagta cctatatcca gttacacccg
     4021 gaaatgcatc caatatcttc cctgctgcta aaggacaaag tacttctgga agatatccta
     4081 acgtagcaac tattcctata gcagttcctg atattgataa tgaataatta ccttcttcaa
     4141 gtaacgaata gtgtaaacct tgaattgcat acatagatac atatattgca acacatccaa
     4201 tcaatagtat ataaatcata gatgattttg ttggtgtaaa tattacacct aaaagacctg
     4261 caatcataag tataaatcca taagatataa tctttgatga accaacttta tctccaagta
     4321 atccagaagc agctgatgct actggtctac aatactgagc taatattgag attgcagatg
     4381 caaacaccac agtagctcca aatgcttcag ttgaataagg cgtaaaataa taaaatgaca
     4441 tattagctga atatgaacaa aacattatta ttgttattat ccatgtatga ggcattttta
     4501 ttactgcaaa aaaggcttct ttatcaaatt ttgctgatac ctctttttca ttatccttta
     4561 atttgaaaaa tactaatact ccaactatta catttattgc cgagtataat actataatag
     4621 ctgtaagacc agatttatca cttacttttg atgaaatata cccaaatagt atcaatacta
     4681 gagatagatg aattgcattg gtaattccac gaccactttc cataaatcca aaagcttttc
     4741 cttgttcatt ttcagatgct aacattctaa cagcttttac aagagctggc caaaaagtaa
     4801 gtattgttgt tattccccat aaggcatgga ttaaaaacac aacaatatat ggaggataaa
     4861 gtgttaatac aagaccagat attcctgtaa taattaaaga acctgaaagc aattttctgg
     4921 ctgaaatacg gtctgctaaa aaaccaccaa aaaggtatga taccattgca agtagaccat
     4981 aaatgcttcc taagcttccc atttgtgtat ttgtaagatt aaatgcttgt gcaaaagtat
     5041 cataataata attctttaaa tatggtaaag catatataaa tgctcctgct acaaagagta
     5101 ttatcaagac tttgaaattt ttccaaatgc ttggtttatt tacttcaact gacatacttc
     5161 ctccttatgt aaatacctgt aaattttcat gataattaat tattgcttat tgtattgttt
     5221 attcaatatt tcggaatatt tattaatata ttaggttaat ctatcaataa tttatggtga
     5281 gttatgagct aaaagtaaac tcttttagct caccataata attatatttt tatttatact
     5341 aacgtccaga gttattagat atcttttttt catagttctt ccatagcatt aaacctgtta
     5401 ttataacaaa aagacctcct cctatcattt caatctcata gattatatta tcttttaaag
     5461 tctccataac tggtgccgtt gagaaaagta tgccaactac agttactaca agtaccattc
     5521 tggcaatatt gatacaaact gaatcacttt ttgcaattga atatggtctt tcttcatttg
     5581 gtcttgtctt tctaagtcta atataagatg taaaaagcaa gacatatgga aataatgctg
     5641 ttaatgctgt catagtaaca agaacattat atatattatc tactgatggt aataagtttg
     5701 ttcctacaag aagtagactc actattatag cctgaactat tactgcattt gatggtatat
     5761 tgtatttatt tgtaacagtc aattgcttag gaaatacacc ttctcttaca tttccaaaaa
     5821 gcatctttac tggacctgct atataaagta ttaatgctcc tagtactgat aaagttattc
     5881 caaatgctac tattcttata aaccaacttc ctatacctaa atcttgtgca actttagcta
     5941 atgcatctag tatccctgta gatgctgcaa ttttatctgg aggaagtatc attgttattg
     6001 ctactgaacc taagatatag attcctccta ttaaaacagc cacagttaat atagctttag
     6061 gaaagttttt cttagcatta tccatttcgg ttatgaaatt agcagtcgtt tcagctcctg
     6121 ataatgcaaa tattatagca gatatagaaa ctaatgaatt ggcatctatt tttggtatta
     6181 tattttggat tgtatatatc gatgctgatg gagctttttt gagaataact acagacataa
     6241 atgccatcac tataagtaat atagcaggaa ttgtagaacc taatgctcct gtattcgtaa
     6301 aaaattttcc aaatgccatc ccacgagtac ttactaagga caacagccaa aatattatga
     6361 gtgaaagtgc taaaataaac atcttattgt ttgataaatc tggatttcca agcatatatg
     6421 caatatttat agctaaaaaa gtcaagaaag aagaatacca aaatatcttt gatgtccaat
     6481 ttagccatga caccataaat ccccacttct ctccataagc ttgtttaacc cattctccaa
     6541 gtccaccatc ttttggatat gttgcagcca actctgcaca tataagtgat gaaggaacaa
     6601 agaatataaa agcaaacaat aaccaaactg gtatagaacc aagacccaat ccaaaactag
     6661 aggcagtaga tttagctatc catctaatcc caaatagtgc tgatatattc ataaatacta
     6721 aatcccaaaa tccaattttc tttttcactg taattcctcc aatttcaaat ttattcagac
     6781 tcatacacaa tgtattgact taaatatcaa gtataagttc ttcaacactt cttggataat
     6841 tagttataat tgtagcacca ttcttaccta ctattgctgt atctgagtgt ctaaatcctc
     6901 caactccagg tatatatatc cctggctcca tagaaaatac cattccttct tctaatatta
     6961 attcattgtc aaatcttaaa tatggctctt catgttctga taaacctaaa ccatgaccaa
     7021 ttctatgatt tgaatataac tctagaccat actttgcaac aacagctctt gctgcttcat
     7081 ccaccattct tgctggtata ccagctttta ttgtatctaa tccagcttgt tgagcctcta
     7141 ctgcaatctt aaacacttct ttttgtcttt ctgttggttt gcctataata aaagttcttt
     7201 cattttctgc tctatagttt tcataccaaa cctgtctact atgtattact atatctcctc
     7261 tttgaagtat tctagtatta gaatctaaat gaggttgtgc tgttctatca atccctgaac
     7321 aagtccagtt tgcaaatcct atatatgtat caggataatt ttcagaaaca tattttagta
     7381 aagcattatc ccctgcaacg tcaaactcca attcactcat cccaactcta acattttcta
     7441 aagacccttt tattcctata tcagataggt aacctgctat cttcaaaaat tctatttctt
     7501 ttgcatcttt tacatatctc atttcaaata ttttacttcc aacatctttg atagcaaatg
     7561 acttatcagt tatatactct gaaaagcttg cagatactat atctttttct attccaacag
     7621 ttgttccttt agggtattta tttaaaatta tatctaagta atgtttatgt gatattccat
     7681 gttctttcat ttctggtatt tcataataca catatacatt atcaactttt gcaacttctt
     7741 tagcatgtaa ttcttctaat ccaggtacta ttaattctat tttatcttga gttactactg
     7801 ttacaattgg tctagaataa gttattgctt taaatccact aatataaaat tgattgtctg
     7861 gttcaaatat taaagcaatt tcaatgcttt tttcattcat gtactttctc aatctttcta
     7921 gtctcatatt atttgacata atgcacctct ttcttaatat tcagataatt tattcaatac
     7981 aacaaggatg aatatattat tttaaaattc ttaatatttt tagtacttgt cgtatttgta
     8041 tgtaaattaa ttttatttgt gattatttta tcataatcat gattattttg tcaatcatga
     8101 ttttatttta aaaaattttt tatattctag tgattttaaa ttatctaaaa tataacgagt
     8161 atacattgta ttttgtaata ttattcttga tttatggtaa gtttaatgat aaaatcacga
     8221 ttatataaac tatattgatt atttgtaggt gaataaaatg acaaataaag aattacaaaa
     8281 gaaaaggata ttgatgtatt tcatagaagc tgctcaaaat attatggaaa acgaaggtat
     8341 agaaaatatt acacttcgta aggttgcaga tatggctggc tataatagtt caactctata
     8401 taaatacttt aaaaatttag accatttaat atcatttgca tctataaaat attttaaaga
     8461 ttataacttg aatatttcaa gatgtataga aaatgtcaat gatgaatata agaaatatat
     8521 aattatgtgg aaattatttt gtaagcattc ttttgataat gctcaagctt tttaccaggt
     8581 attttttaac ttgtctagtg atgaacttag ctatataaca aaacaatatt atgatatgtt
     8641 tccagaagat ttaggtattc atgatagtga catatcttta atgattacag gagaaagtat
     8701 aaaggaaaga aataagatac tattaaacaa tcttgtagat actggatata taccagctaa
     8761 agatttagac ataatcaatg atataattat ttctttttat caaaatctat tattcttaaa
     8821 aaagaacaca ggaaaaaatt ctgatgatga tgaattaact atgaaaatga tttcttctat
     8881 tgaatacata ataaaacatg ctaaataaaa ttaaaatatg gctgtctcaa aatagagata
     8941 agcctttttt attgttgaaa taataaaaat atatttaatt tcatataaaa atgagtgcct
     9001 cgtaaactaa aaagttcatt ttgagacacc cctaaaataa atttattatt tattatacaa
     9061 tagtttatat atcatcacct aaaactactt cttccatttc atgcttttta cttctcatca
     9121 ttagttctat tccaacttct ttaggatttg aacctttctt tattacagaa tatatagcat
     9181 tagttatagg catatctata tctaattttt cagcaacatc atgtgctact tcagtagcag
     9241 ttatgccttc tacaaccatc tttacttctt ttaaagtatc ttctaaactc atccctttac
     9301 ctatgagtat accagctctt ctatttctac tatgcatact tgtacaagta actattaaat
     9361 ctccaatacc tgaaagacct gcaaaggtag acatattagc tcccatagca attccaagtc
     9421 tgctcatttc actaatacct cttgtcataa gggcagcttt tgcattatca ccatacccta
     9481 atccgtcaca tatcccagct ccaaatgcta taatattttt taatgctcct ccaagttcta
     9541 ctccaactat gtctggattc gtataaactc taagtttagg gctcataaat aaatcttgta
     9601 tcatctgtgc tatttttaaa tctttagaag caactactac ggtagtagga atatcccttg
     9661 ctacttcttc agcatgagat ggcccagata gtattacata tggattttgt ggtaattctt
     9721 cttcacagac ttgagataat ctaagcccag tccctttttc aagaccttta gctacattaa
     9781 caataacttg tccttctttt ataaatggtt ttattttttg gcaagtactt cttacagcct
     9841 gtgatggaac tgctaaaact atcaccttac aatcttttat agttttttct atatcagtac
     9901 ttatagttat attatttgga aataatactc ctggtaaata gtctgtattt tctttagttc
     9961 tgtttatttt tttagcttgt tcctcttttc gagtccacat gctcacatca ttaccttttt
    10021 ttgcaagccc taaagctaat gcacttcccc agcttccagt acctaataca catactttct
    10081 ccatgtttaa caccacctga gtataatata tttactattc tttcttttga ccaatttttc
    10141 tctctgtccc atttaataat cgctttatat ttgctctgtg attatatatt actgatgcag
    10201 ttaagaacag agctactatt aaaccagctt tattttttac cataatcata aatattggtg
    10261 aacatcctat tcctacaaca gaacccaatg aaacatactt tgttatagct accaccaata
    10321 taaatactgc tagacacatt agtgttatta ctggatttac agctagcatt gcccctagtg
    10381 aagttgcaac acctttgcct cctctaaatc ctaaaaccgc tggccaatta tgacctgcta
    10441 caacacatat aactgccaga tatccagcta aaagagtatc tattccaacc aatcttgctg
    10501 caaattctga tattaaaaca gccattacac ctttaagaac atcacctaaa aatgtcattg
    10561 ctcctgctct ttttccaagt gtcctaagta catttgtcga acctgcatta ccagaacctt
    10621 gagtccttat atcaacccca gcaattcgct ttgcaactat gtatgaggta gatatatttc
    10681 ctaaaagata tgcaactaca gcaataatta tataactaaa tatctccact gaatttcccc
    10741 cctttagtta tattttaaga tatttatttt ttattttttt gtctatactc aaactttatg
    10801 gaagttcctt caaatccaaa attttctctt atcttattct caaggtatct ttgatatgag
    10861 aagtgagtta aatccttatc atttataaac aatgtaattt taggaggtct aatgtcagtt
    10921 tgagtcccat agtaaatttt taatcttcta cctttgtcag atggtggttg atttaacatt
    10981 acagcttctc caattacttc attcagtgct gaagttgata ttctcttaga atgttcattt
    11041 gacacatatt caactgtatc tagtatcttg ttcattcttt gattagtttt tgcagaaaca
    11101 aatacgattg gagcatacat cataaatgga aatttttctt ttatatcttt tgtgtaatta
    11161 ctcatagtct tattatcttt ctctattaaa tcccatttat tgattacaaa tatgcatcct
    11221 tttccttcat catgtgctat acctgcaacc tttgtatctt gttctgttac accttctaag
    11281 gcatctatta ctatcaatac tacatctgct ctatctacag cactcatagc tcttattaca
    11341 ctgtattttt ctattgtctc atatatttta ctttttcttc taagccctgc tgtatctatt
    11401 agaagaaatt tctgtccatt tttttcaaaa taagtatcaa ttgcgtctct agtagttcct
    11461 gcaattggac ttacaattac tctttcttct ccaagtatct tattgagtat agaacttttt
    11521 cctgcatttg gtttacctgt tatagctact cttattatat cttcttcata ctctgtatct
    11581 aatcctgctg gaaaattttc aactatctca tctaataaat ctccaaatcc catactattg
    11641 gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat aaaaatcgta tatattatca
    11701 aattgacttt gactatcaat cttattaacc actaagatta ctggtttttt tgtctttcta
    11761 agcaattgtg caatttcttt atcagcagct gttattccag ctttaccatc aactataaaa
    11821 agtattacat gtgacatgtc cattgcaagc atggcttgat ttctcatttg agataatatt
    11881 atatcttcgc tatcaggttc aattcctcct gtatccacta atgtaaaata tttatctaac
    11941 cattctactt cagcaaatat tctatctctt gtaactccag gtgtattttc aactattgat
    12001 atcctttttc ctgcaaattt attaaatatt gtagacttac caacgtttgg tctcccaact
    12061 acagctacaa ctggcctact tatactcatt ttatttctcc ttccatcttt tgttttctat
    12121 tttaaaattt tatccacaaa atcttttcct tcattcaaac aagggataat ttttattctc
    12181 ataagttctt ctaattgatt taattcaata ttatctaaga aaatttcttc atctgctttt
    12241 agcatacttc ttgttatata aagagcttct cctaattctt tatcttcaag ttgttctttt
    12301 aaatccttag ctgttaaaag acctgataca gttattgtct ctccaaaaaa cttgtttttg
    12361 atttcatata catttacttg aagattttta aacttgtcca tcatagcatc tgccatactc
    12421 tgtataaatt catatgcaga atgaccagta gctattgata ctttcttctc tctttttaga
    12481 atcttctcag gcaatatatt tagatactct tcaatctctg tttttaactt acgtatcata
    12541 cctacaccat cttcaaactg taaaaaacct tcatattctt catatccagg caattcacta
    12601 ttagatagta tataaaattc atctgataaa aatgcaaatc gagtacctaa tttttcaaga
    12661 tatttctgtt gtatttgatg tatttgctct atagttttac ttgcgctctt atcattaaat
    12721 atatttaatt ctactagatt ttctctatgc tttgtaatcc caactggaac tatcgcaaca
    12781 ctgtttacat acggataaag ttgagctagt tcttttattg ttctatccaa ttcctttcca
    12841 tcatttatac ctggacataa aacaatttga cagttcattt ctatatgagc atctgccaat
    12901 ctcttcatta taccatataa ttttcctgca aatttattgc ttatcatttt ttgtcttaac
    12961 tctggattag ttgtatgcac agatatattt ataggactta ttctatattt tataatatta
    13021 tttatatctt cttcactcat atttgtaagt gtgacaaaat ttccttgtaa aaaagataat
    13081 ctcgaatcat catctttaaa ataaagagtt tctctcatac cttctggcaa ttggtctata
    13141 aaacaaaaca tacacttatt tctacagctt ttagcttggt caattatcgg atttgtaaat
    13201 tcaacaccca aatcttcatc atagtctttt tctatctcgt atatgtagac ttcgccatct
    13261 tttttttgta tttctacatc taaatattca tcacttagta agaatctata ctctattata
    13321 tcatgtattg gctgttcatt cacagatata agtaaatctc ctacctctat acccatttct
    13381 tctgcaatac tatctttata aaccttactt ataatattat ttactttttt tacatttact
    13441 tccaatattt tcaccactct tttgtattca tcaatgctta ttataatgta ttttaatatg
    13501 caatttattt atttattata ttttatgata gcatttttaa gcatttcatg tcataataac
    13561 acattttaat tctaatagtc aatataagta aatagctata aaatagatta gaacaaataa
    13621 tattcaaatt taagtttctt aaaaatttaa atattatttg ttcttaaaaa aattttataa
    13681 atttaatgtt ttactatgat taacgtccca tttgtcatat catgaaccat tccacctaca
    13741 cactttgaca tcaacaatgc cttattttgt aagtctttct catcatttgc ataaaatatt
    13801 ggacaacctc caccattttt catattctta tcaagtgtaa ctatagctag ggtatattca
    13861 tttaaaccta tatccattcc catcttatgc acttcctttc tttcctattt tataattttt
    13921 tagtgataag ttttttcctt tagagcttga aagtattggg catgacttta cagcttccat
    13981 tactttttcc acattcttat ctactggtaa ataagcaatc attatacttt catcctcttt
    14041 atttcttcta ggtaatggta cgaatgaagg ttctccataa tctccatata aacctaacct
    14101 tgaatatata ttgtatacta ttgcttgtct ttgaccggag tcaaataata ttcctgcatt
    14161 agcataatcc ttatttttag gaattatttg tattcctaca cctctattaa tatatacttc
    14221 tctatcactt tttagaccta tatttgttat tccctttaaa tctcctacct tcaatataga
    14281 ctcatctaca aaactaatct cagcaggcat tacatcagct atgtctccaa tactttttct
    14341 agtaagaagt ttttttaata taaaggctaa cactaatcca caaattgaac ttattaccaa
    14401 actcattcct tcacttattt tcgtttctgc aagtattaca taatacaatc ctattgttaa
    14461 aaaagatgta actatacaca tataatttct tacttcatag gttctagcta tctcttctat
    14521 gaatgcagct cctctcttta caagttgaac ttcctctaag ttttgcaatg tatttcttct
    14581 atttgctctc acttgtctaa attgctctgc tgctagggat aaaaaagtta tagatgtata
    14641 agaacgattt ataagagctg gaaccaataa tgctcctaat gaagaagcga cgaatgctaa
    14701 tataagttga gatagtaaaa catttggttg cgttggatac tgtttttggt ctaaattcaa
    14761 cattataatt ctagatgtta taccaataat aacagcaaca aaaaatactc tatccattaa
    14821 atcactccta aaatatttaa tttgaaattt tacatgtaaa tctacaaagt ctttctagaa
    14881 aaatcactct gctttccttt tgatgtttct atgataggtg tacttttcac tacatctatc
    14941 atgacatcta tatctttgag tattggcata tatggtataa ccacagtact tttttctaaa
    15001 ttggtcttag atatagcaac aatatcaacc tcatctacat ctctatctat ccccatatga
    15061 ataaatagat tatgtattat agcttgtctt tgccctatat catttactat tccaaatgca
    15121 cctaaatctc ttggtataac ttctatagcc agacattcat ttctatactt ttctcttgta
    15181 ctttctagac ctatgttagt tattattaca ccatttacca ttaaaatagg tccttcaaaa
    15241 cttatttttg caggaactat atctgctata tctgctatac tgttccttct taaaaatctt
    15301 ctaaaaatca aaccaacaat agctcctgac acaatagcta agattgtgca ataaaaaaag
    15361 ctaaggccat actttcttgc aaaaacgata taaactatcg aagataccag tgcagaaaaa
    15421 agacttatat aacttctaga ttcatacgta gatgctattt cttcaacgta agcgtctcct
    15481 tttggtacta attcttcatt atcgatattc tttagtgtta ttctttcttg ttctgcaaga
    15541 ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg ctgcaaactc cttatctata
    15601 agtgcaggta atgctatagc acctaacgaa gctgaaagcc cagatataat tatctgttct
    15661 aaataatctt gtggtcttga tgggtattgt ttgtctgtaa ctcttaaaac taatcctcta
    15721 cataatattc ctacaactaa tgcaactata aaagagtgtc taaataaatt tgcattcaat
    15781 aaactttctt ccattttctc agctccttct cttatatagt ttgtgcaaaa aacaaaaaaa
    15841 attcccttct atttaaggaa atttttttgt aatattgtaa ctataaattc gctaccaatg
    15901 ccaagttcac tttttacttt tatacatcca tttagtgatt tgactatatg ctttgttata
    15961 gccaatccta aacccgttcc tcctacatcc ctacttcttg ctttatctac cctataaaat
    16021 cgttcaaata tgcgttctac atcttctttt ggtatcccta caccattgtc tataacttta
    16081 attacgattt tttcttcatc ttttaacact acaacttgta caatcccatt atcttgggta
    16141 tactttatag cattatcaac taggtttaag aacacttgct ttatatagtc tttattggat
    16201 agtatactta ttgatttatc atcacattta tataaaactt ctatattttt ttcactagct
    16261 gaatgttgag ttatttcata tacctcttta aatacatcat atactacaac attttccata
    16321 agaagagtat ctttattttc tacaaatgat aacagcaata tatcatcaat caaccttttt
    16381 aatctatctg attcgctttc tattatttct aagaatctat ttctagtatt cacatctata
    16441 ttttcatttg actttagtgt ttcaacaaac ccacttatag atgtaagtgg tgttttaagt
    16501 tcatgactta catttgcaac aaaatcactt ctcatatttt caagtttaac ttttttagta
    16561 atatcttcta tatttactat cgaacctatt attacgttgt tagtattctg aagataaact
    16621 gggtctactt ttatcttata tacgatatca tctttagtag taatattagc agtttcatta
    16681 tctttagaac caataaattg aagtatttgt tttaatattt cttcttcttt tattacaaca
    16741 ttaatattat gaccttcaac taattgattt ggttcactct ttatcatttc ttttgcttca
    16801 tcatttatca gaagtatatt tccatttaca tctatagcta aaattccatg agatatactc
    16861 tttaatatag accttagctg taaatgttta tactgtactt cttctaaggt tttatccata
    16921 ttctttatca tatgattaaa atttctagct aattcgccaa gttctccttt ggctgatatg
    16981 ttaagcctag catgatattc attattactt actttttttg agacgtatat aaactctttt
    17041 aaatatttcc ttaatgttat tgtgt
//
LOCUS       DAELJO010000069        16891 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELJO010000069 DAELJO010000000
VERSION     DAELJO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.069"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            495..1205
                     /locus_tag="KPB88_001952"
     CDS             495..1205
                     /locus_tag="KPB88_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054232.1"
                     /translation="MIKIAICEDEKEQQELLKRYINQIFDVLSVKCRLEVFNSGEELL
                     ERYSKDTDILLLDIQMGQINGIDTARKVRVLDDKVEIIFITALIEYALEGYEVRAYRY
                     LIKPVKYNNLKDNIINCIKEVDIKNKYIVVKVQGSQTKLNVNEIAYIEVQKETITIHT
                     LNEVYKINGTMSNIEKEIDCSRFFRCHKSFLVNLEHVKSIKQYVAILENSKEVPVSRY
                     RFKETKDKFFDLIEEKLC"
     gene            1199..2536
                     /locus_tag="KPB88_001953"
     CDS             1199..2536
                     /locus_tag="KPB88_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0054233.1"
                     /translation="MLDSVYSLAIINMVIFIALFFMLMNKTGYTYRCKTNIQLILILI
                     LSAILIINGMPFGIVPLHALIRIILILSLICKITYQINIKKATINSVIYSLLYILIGC
                     SIYWILQFSWKYVGHYILKVNTVINFIVIFIGVYFFDILHKIYNNKKYYLYIILTIAV
                     NVVIVLFLNISTEKMEDLYTIVTKNHIEYSYNVYTMYFSLFMKNTFPYILFIVNVLLI
                     SIFINSIKSEKEKIKAEFVSEKLDMQYKYYFTVKESQEKMRQVYHDMNNHMKNIKSLK
                     NSSEDVNKYIDGIEDEVKNTKNIYNTGNVLLDIILYEKSRLCMENNIDFNVGIDFSKC
                     EFIEMIDVSSIFSNLIDNAIEACSNIDDNNIEKYITIKSTFIKGYYIIRCENSKMNKV
                     IIKNNKIFTSKKDKFLHGIGIESIKSSIRKYNGELKIKDSEYKFIVTAHIPIE"
     gene            2717..5284
                     /locus_tag="KPB88_001954"
     CDS             2717..5284
                     /locus_tag="KPB88_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0054234.1"
                     /translation="MTTFKLAVSYMKRQKGKTVALLSCIVLAVMLIFSMIVIRDSGYD
                     SQIKEAKDLHGDYHIWFEGLEKDKTQDLTNEEGISKSNTSKELCEVVDKESGVKLYLN
                     SFDKGFLSSLGYKIEGREPIKDGEIVIEKEATRQMGISNPLNKNIDLMLLNKYIDDNE
                     INHMDSENKTFKVVGVIEKLDKYYDKSRYSITLRAFVYKDSNLPIKIKDTYNGTIYLK
                     SEKNIPQFITKMTKKIDTNVFNLHENGEVDLAKNQRQNSKFSKENIINSVLLVIVSTI
                     VIYNIFNIIFQDMTSQIGLMKSIGMSNKKVRNMLIIMSFIYIILGTLVGIVFGMIFSY
                     VGLRVVYGYSSMLIIQISSIICSFAVSIISVFISSFIVIKKSRKMSIIEAIRSSDKYE
                     KKSKNDNKKIDGKSKNLLINMATRNLWRNKPRTILTMLAITLVGIMFILNLGAKSLLK
                     KNMEEGITGGSWSMSYGSVDKTVEGDSRSSESLFYKLDNNLIQKVNGMKGVRHVEPHF
                     YNHRGHILLSKNKLSKAYQDELDRKNSSYQEEHNNEYPLLIRGYSDDMLKQRQEFIEK
                     GENILSTTSGKYKKVILVNNTNSQVTHSFDAKIIDDVKIGDIIEIKLPVYRDGIEKYE
                     NFKVEVGAIMKELYASSQDGNTQAQGAQMIFKENDYKELTGQKEYNKLFVIVEKGQLY
                     SVERRLEETTKNYGSTEINGKGEELKLIGMQQSSEERLSVIYQFLTILILAVNIIFIM
                     RSNIITRRKELATLRAIGMSIKSIKKILIIESQMYGMVASIIGAIIATVYHNYGLAKT
                     NKVLLEGGYTRIIEPNIPFSQIIILFTIFIAMGFISIYASKDKIEGTTITEGILQNN"
     gene            5358..6026
                     /locus_tag="KPB88_001955"
     CDS             5358..6026
                     /locus_tag="KPB88_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054235.1"
                     /translation="MKKLAIENIKKVYGKGDNKVYALNGIDLEIQPNKFTTIVGQSGS
                     GKSTLLHCMAGLDKPTDGKVFIDDLDLYTLNDDKLSKIRREVFGFIFQSYNLIPVINI
                     YDNIVLPISISRRKIDKDYIIDLIGKLGIKSQINKFPNELSGGQQQRVAIARALANKP
                     SIIFADEPTGNLDSKTTDEVVRLLKLCVYEYKQTLVMITHNAEIANNSDNIINISDGK
                     IVSI"
     gene            6289..7149
                     /locus_tag="KPB88_001956"
     CDS             6289..7149
                     /locus_tag="KPB88_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0054236.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            7139..9010
                     /locus_tag="KPB88_001957"
     CDS             7139..9010
                     /locus_tag="KPB88_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0054237.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            9030..10484
                     /locus_tag="KPB88_001958"
     CDS             9030..10484
                     /locus_tag="KPB88_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0054238.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            11509..12717
                     /locus_tag="KPB88_001959"
     CDS             11509..12717
                     /locus_tag="KPB88_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054239.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            12748..14127
                     /locus_tag="KPB88_001960"
     CDS             12748..14127
                     /locus_tag="KPB88_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0054240.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            14233..15423
                     /locus_tag="KPB88_001961"
     CDS             14233..15423
                     /locus_tag="KPB88_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0054241.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(15551..16879)
                     /locus_tag="KPB88_001962"
     CDS             complement(15551..16879)
                     /locus_tag="KPB88_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054242.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIKIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
BASE COUNT         6573 a         1746 c         2955 g         5617 t
ORIGIN      
        1 gatttatgac aaaatagaat gatttttatt aaaatttatt taaaaatatc actagagtgt
       61 ttaaataaaa ctaaacttaa gattttatat ataaacatat aatttatgat atctgaaaat
      121 tttatagtat ttaagtaaat aaaaatatat gtaataaatg aaagtagtgt atgattagtt
      181 tatttatctt taagattatg aagcatatac aagtttatat attaaatata tagaatatag
      241 ttttataatt tgctaaaata aataaaagta tatattagga aaggttgatt tatgaaaaat
      301 atatacgtaa tattatctta aacattaaaa gaatatactg cataaaaaat aataatcatt
      361 tatgagtatt aaataataac tcataaataa ttattattta ataataaagt acgtaactta
      421 ataagaaggt taagaaaaat tttagctttg ttaagaatga tttaagtata taaatgtgta
      481 tggggtggaa ttagttgata aaaatagcaa tatgtgaaga tgaaaaagaa caacaagagt
      541 tgttgaaaag atatataaat caaatatttg atgtattatc tgttaaatgt agattggaag
      601 tatttaattc aggggaagaa ttacttgaaa gatattcaaa agatactgat atacttttat
      661 tagatataca aatgggacaa attaatggaa tcgatactgc gagaaaagtt agggtactgg
      721 atgacaaagt agaaattata tttattacag cattaataga atatgcacta gaaggttatg
      781 aggtaagggc ttatagatac ttaataaaac cagtaaagta taataattta aaagataata
      841 taataaattg tataaaagaa gtggatataa aaaataaata tattgtggta aaagtacaag
      901 ggagtcaaac taaattaaat gtaaatgaaa tagcatatat agaagtgcaa aaagaaacta
      961 taactataca tactttaaat gaagtttata aaataaatgg tacaatgagt aatattgaaa
     1021 aggaaataga ttgtagtaga ttttttagat gtcataagag ttttttggtt aatttagagc
     1081 atgtgaaaag tataaaacag tatgttgcta tacttgaaaa tagcaaggaa gtacctgtta
     1141 gtaggtatag gtttaaggag acaaaagata aattttttga tttaatagag gagaaattat
     1201 gttagatagt gtgtatagtt tagctattat aaatatggta atatttatag ctttattttt
     1261 tatgttgatg aataaaactg gttatactta tagatgcaaa actaatattc aacttatatt
     1321 aattttaata ttgtctgcaa tattaattat aaatggcatg ccttttggga ttgttccatt
     1381 acatgcttta ataagaataa ttttaatatt gtctttaatt tgtaaaatta cttatcagat
     1441 taacataaaa aaagctacta ttaattccgt tatatatagc ttgttgtaca ttttgatagg
     1501 gtgtagtata tattggattt tacagttctc atggaagtat gtagggcatt acatacttaa
     1561 agtaaatact gtaataaatt ttattgttat ttttataggt gtttattttt ttgatatact
     1621 acataaaatt tataataata agaagtatta tttatatata attttaacaa tagcagttaa
     1681 tgtagttata gttttatttt taaatatatc aactgaaaaa atggaggatt tatatacaat
     1741 agtaacaaag aatcatattg aatatagtta taatgtatat acaatgtatt tttcactttt
     1801 tatgaaaaat acatttccat atattctatt tatagtaaat gtattattga tatctatatt
     1861 tataaattct ataaaatcag aaaaagaaaa aattaaagca gaatttgtaa gtgaaaagtt
     1921 agatatgcag tataaatact attttacggt aaaagaatca caagaaaaaa tgagacaagt
     1981 ataccatgat atgaacaatc atatgaaaaa tataaaatcg ctaaaaaaca gtagtgagga
     2041 cgtaaataaa tatattgatg gtatagaaga cgaagtaaaa aatactaaaa atatttataa
     2101 tacaggaaat gtactattag atattatctt gtatgaaaaa agtagattat gcatggagaa
     2161 taatatagat tttaatgtag gcatagattt tagtaaatgt gaatttatag aaatgattga
     2221 tgtaagtagt atattttcaa atttaataga taatgcaata gaagcatgta gtaatattga
     2281 tgacaataat atagaaaaat atataactat aaaaagtacc tttataaaag ggtattatat
     2341 aatcagatgt gaaaacagta aaatgaataa agtaataatt aaaaataata agatttttac
     2401 ttctaaaaaa gataaattcc ttcatggcat aggtattgag agtataaaat cttcaataag
     2461 aaaatacaac ggagaattaa aaattaagga tagtgagtat aaatttatag ttactgccca
     2521 tataccaatt gaataaaaat tcaatagatt gatgcattta aaaagcttat aagctagtct
     2581 ttaatatgct agttggcggt gaaaaaatac cacttgtcgg acttatattg aataaaaatt
     2641 ttaaaaatgt tacctttaca aaaaaggtag cattttttta tacaaggatt taatagaaga
     2701 agaaggaggt aaagttttga ctacatttaa actagcagtt tcttatatga aaagacaaaa
     2761 aggaaaaaca gtagcactac tatcgtgtat agttcttgct gtaatgctta ttttttctat
     2821 gattgtgatt agggattctg gatatgattc tcaaataaaa gaggcaaagg atttacatgg
     2881 tgattatcat atatggtttg aggggcttga aaaggataaa acacaagacc ttacaaatga
     2941 agaaggtata tcaaagtcaa acacttcaaa agagctatgt gaagttgtag ataaagaaag
     3001 tggtgttaag ctttatttaa attcatttga caagggtttt ctaagttctt tgggatataa
     3061 aatagaggga agagaaccta taaaagatgg agaaatagta atagaaaaag aagctacaag
     3121 gcaaatgggg atatctaacc cattaaacaa aaatatagat ttgatgcttt taaataagta
     3181 tattgatgac aatgagatta accatatgga tagtgaaaat aaaacattca aagttgttgg
     3241 tgtgatagaa aaattagata aatattacga taaatctagg tatagtatta cacttcgagc
     3301 ttttgtgtat aaagactcca atttgccaat aaaaataaaa gatacatata atggaacaat
     3361 ttatcttaaa tcagagaaaa atatacctca atttataact aagatgacaa aaaagataga
     3421 taccaatgtc tttaatctgc atgaaaatgg tgaggttgac ttagcaaaga atcaaagaca
     3481 aaattcaaaa ttcagcaaag aaaatattat aaattcagtg cttttagtca ttgtatcaac
     3541 tattgtaata tacaatattt ttaatattat ttttcaggat atgactagtc aaataggtct
     3601 tatgaaatct attggtatgt caaataagaa agtaagaaat atgcttatta ttatgagctt
     3661 tatctatata attttaggaa ctctagtagg aatagtattt ggaatgattt tttcatatgt
     3721 tggtttaaga gtagtgtatg gatacagctc tatgctaata atacaaatat ctagtataat
     3781 atgttctttt gcagtttcaa tcatatcagt atttatttct agctttatag taattaaaaa
     3841 atccagaaaa atgtctataa ttgaggctat aaggtcaagt gataagtatg aaaaaaaatc
     3901 aaaaaatgac aataaaaaga tagatggaaa aagtaaaaat ttacttataa atatggcaac
     3961 tagaaatcta tggagaaata aacctagaac aatattaaca atgttagcaa ttacattggt
     4021 tggaataatg tttatcttaa atctaggtgc taaaagtttg ctaaaaaaga atatggaaga
     4081 ggggataaca ggtggttcat ggtcaatgtc atatggaagt gttgataaaa ctgttgaggg
     4141 agattctaga agttcagaat ctttatttta taaattagac aataatttga tacaaaaagt
     4201 gaacggcatg aaaggtgtaa ggcatgtaga accacatttt tataatcatc gtggtcatat
     4261 tttactttct aaaaataagt tatcaaaagc ttatcaagat gaattagaca gaaaaaattc
     4321 ctcttatcaa gaggaacata acaatgaata tccacttttg ataagagggt atagtgatga
     4381 tatgttaaag caaagacaag aatttattga aaaaggggaa aatatactaa gtactacatc
     4441 tggcaagtac aagaaagtca tcttagttaa caatacgaat tcgcaagtta cacattcttt
     4501 tgatgcaaag ataattgatg atgtgaaaat aggagatatt atagagataa agctacctgt
     4561 atatagagat ggtattgaaa aatatgaaaa ctttaaggta gaggttggtg ctatcatgaa
     4621 agaattatat gcatcaagtc aggatgggaa tacacaagct caaggggctc aaatgatatt
     4681 taaagaaaat gactataaag aattaacagg tcaaaaagag tataataagc tttttgtcat
     4741 agttgaaaag ggacaattat attctgtaga aagaagatta gaagaaacca ctaagaatta
     4801 tggatctact gaaatcaatg gaaaaggaga agaactaaaa cttattggaa tgcaacaaag
     4861 ttcagaagaa agactttctg taatatatca gtttttgact atattaatac ttgcggtgaa
     4921 tattattttt attatgagaa gtaatattat tacaagaaga aaagagctgg caaccttaag
     4981 ggcaattgga atgagtataa aaagtattaa aaagatacta attattgaaa gtcaaatgta
     5041 tggaatggta gcttctataa taggagctat aattgcaaca gtatatcata attatggttt
     5101 ggctaaaacg aacaaagttc ttttggaagg tggatatact aggataatag aacccaatat
     5161 tcctttttct caaataatta tactatttac aatttttatt gctatgggat ttatttctat
     5221 atatgcatca aaagataaga tagaaggtac aacaataaca gaaggaattt tacaaaataa
     5281 ttaaggctat ggaatgacaa gataaaaatg taatactatt tagtgggatt taaataagaa
     5341 gaatggagaa ccatattatg aaaaaattag ctatagagaa tataaagaaa gtatatggaa
     5401 aaggggataa taaagtatat gcattaaatg ggatagattt agaaatacaa cctaataaat
     5461 ttacaactat agttgggcaa agtggctcag gcaaaagtac attacttcac tgtatggcag
     5521 gtcttgataa acctacagat ggaaaagtat ttatagatga tttagattta tataccctaa
     5581 atgatgataa gttatcaaag ataaggagag aagtgtttgg atttatattt caaagttaca
     5641 atctaattcc agttataaat atttatgaca atatagtact tcccatttca attagtagaa
     5701 gaaaaataga taaagactat ataatagatt taataggtaa actaggtata aaatctcaaa
     5761 ttaataaatt tcctaatgaa ttatctggag gtcagcaaca aagggtagca attgctagag
     5821 cacttgcaaa taaaccttca ataatatttg cggatgagcc tacaggaaac ttagatagta
     5881 agacaactga tgaagttgtg agacttctaa aactctgtgt atatgagtat aaacagacat
     5941 tagttatgat aactcacaat gctgaaatag ctaataattc agacaatatt ataaatatta
     6001 gtgacggtaa aatagtatca atttagtata taaaccttgt cagataatcg cataacagag
     6061 cttgtagtta tagaataaaa aaatatctag gaaaccgttg acagatttat atggatagta
     6121 taacatatag aatatgaaat taaacaaaaa agaattttaa ttatggcgac gtagtcataa
     6181 ttttgagtat gttactgatt agcaggcaga actcaagtat atatgtgaaa aatagaggga
     6241 tactctatac atatatgctt gagttctttt tattgaaggg aggaatatat gaaaataatt
     6301 cagattttaa ataataatgt agccttagta aaaagaggta aaaatgaaat tattgtattt
     6361 gttaaaggaa ttggctttaa aaaaagagtg ggacaagtaa taaatgaaaa tgaaatagag
     6421 agaacatata tactagattc atacgatatg ctagaacact ttagttatct actacaaaac
     6481 tcagactcca ataatatagt tcttattaat gaaataattg aatatggtga gaaagcttta
     6541 aatataaagg caagtgatta tctaagctta accttactag accatttaga gtttttactt
     6601 aaaagatgtg aaaagaatca atttataaaa agtcctttgg tatggaatgt taaaagattt
     6661 tatccaaagc attttgaaat tggaatgcat gcactaaaat taattggtga tagtaaggga
     6721 attaatcttc ctgatgatga ggcagtatca atagcacttc attttgtaaa tatggaggta
     6781 aataaagagt ctcatgatag tacaattgta gagttgagaa ctttagcaga tatagtttct
     6841 ataataaaat atcattttaa tgtagagcta gatgaaactt caacaaatta tatgagattt
     6901 actactcatc tacagtactt tatccaaaga attatgtcgg atgaagcttc aatagatgaa
     6961 gataattcta tagatttata taaacaagta agtcaacttt atccaaagtc atttggaagt
     7021 gttcaaaaga taagaaaata tataaaatca caatttaatg tcgatatatc tataagtgaa
     7081 gaaacttatt taatgttgca tataaatagg gtgactcaaa gactggaggc aaaatcagat
     7141 gaaatataaa gatttaaatg aaaatataat aaaacttata gggggaaaag aaaatataaa
     7201 gtcggtagcg cattgtgtta ctcgtttaag atttacttta aatgatagga gtatcgctca
     7261 aacagaagaa attaaaaaat taaaaggagt aattgatgta gtttctaatg atgttgctta
     7321 tcaaattata ataggtactc atgttgtaga tgtatataac gagtttatgt ctatgatagg
     7381 tttaagttca agtgaatcaa gtataaaaag tgaaagaaaa aagggaatta gaggtatcaa
     7441 aggtatattt aattccatct ttgtggtagt ctcagagact atgacttcag taattgaagt
     7501 actacttgca gctggtatac ttgcaagtat attagcactt ttaaatatgt ctggcatact
     7561 tcagagtgat agtcctacat atcaaatatt agatacatta agaagtgcta catttcactt
     7621 cttaccagta ttaattgcaa tagcatcagc aaaaagactt aatgtaaatc cctatatagc
     7681 agtagtgtta gcagttacgt tattatcttc atcaatagat ggtgtagaag gattaagttt
     7741 atttggtata aatttacagg ctacaactta tgcaaacagt tttattccaa tacttttagg
     7801 agtatgggca atgggaatta taattgaagg tttgaaaaaa atattaccta aagcattaca
     7861 ctatttttta gttccagtat tatctttaat aataactcta tcaattacat taactgtatt
     7921 tggacctatt ggtatgtgga ttggtgatgg attaaactat gcatgtatgt ttttaattga
     7981 tacattgggt aattggagtg ttgttgcact gtatgcagct ttacaaccac tcttaatagt
     8041 ttttggtgca gcaaacttta ctatgcctat atctttaaat tctgtgacaa cattggggta
     8101 tgacccaatt tttatgggcg ctgcaacaat atctgatatt gcagtttgtg gtgcaatgtt
     8161 tggatatttc ttaaaaacta gaaataaaga acaaaaacaa ttatttggta cagtaagttt
     8221 tagtgcactt atgggagtta cagagccttc agtgtttggt gtgtttatga aatatagaag
     8281 accatttgta gcagtaatga taggtggagg tataggtggt ttaatagcag gtttagctca
     8341 agttaaaaca tatggaatgg tttggggatt agctgcatta ccaacatatc ttgttggagg
     8401 agaggtatct aactttacat ttatgataat aagtgtagta gtatcatttg tagcagcaac
     8461 agcagcttcc tatttactag gaataccttc agatgaaaaa gaagaagtta agggtgataa
     8521 tgaaaaagaa ttagaactaa taatcaacga gaacaattca agaactatta gtattggaaa
     8581 tgttgcaaaa ggtcaggtaa ttgcacttgg ggaagttaat gacaaagcat tttcatctgg
     8641 agcattaggt aaaggggttg gagttatacc tagtgaatca aaatcaactg ttatatctcc
     8701 tgtagatgga actataacta tagtatttcc aactaaacat gcatatggta tagtgactga
     8761 tgaaggatta gaaatattaa ttcatattgg gatagatact gtaaacttag atggaaaatt
     8821 ctttgaaagt cttgtaacac aaaatcaaag ggttaaaaaa ggagatgctt tagcagtatt
     8881 tgaatctgat aaaattatag aggaaggctt tgattctact ataataactg tagttacaaa
     8941 tactagcgat tatttagatg taatctcatc aaatgatgaa tcagaagaat tattaactgt
     9001 aatagtataa attttaggag ggaaaattca tgaatattaa agatacattt ttctggggag
     9061 gaagtattgc agcccatcaa tgtgaaggtt catgggatag tgataacaaa ggacctgcaa
     9121 ttatggactt tgtaactaag ggctcttatg aaacgccaag agtaattact gataaaatag
     9181 aagaaaaatt agattatcca tctcataatg gaatagattt ttataataga tataaagaag
     9241 atatagcttt atttaaagaa atgggattta gtgcattaag aatatctata gattggtcaa
     9301 gaatttttcc aaatggagat gatgaaaatc ctaatgaatt agggattaaa tactatgaag
     9361 gtctgataga tacattaata gaaaataata tagaacctat tgtaacacta tatcattttg
     9421 aattacctat gaatttagta cataaatatg gttcatggaa taacagaaaa cttatagatt
     9481 tgtatttaaa gtacagtgag actgtaataa gaagatttga tgataaggta aagtactggg
     9541 ttacatttaa tgagatgaac catattgacc ctcaaacaga gcactcggat atttttactt
     9601 atatgatagc aggtattaag tatagtgaaa tagaaaataa ggttcaaaca ttggcaaatg
     9661 tagggtataa tatgactttg gcgggtgtaa aggttgcaaa gttggctaga gaaatcaatc
     9721 ccaacaataa agttggatgt gtatttggac taaatccaat ttatccatac aattgtaatc
     9781 ctagtaatgt gttaaatgca tttttagata atgaccgtga ttattatcaa attgatgcaa
     9841 tgtgcaatgg gaagtttcca caatataaat taaaagaata tgaaaaaaat aatataacta
     9901 ttgaagtgac tgatgaagac aaaaaagcat ttaaagaagg taagttagat tttataggtc
     9961 ttaattatta tttttcaagt gtatctactc caaaagaaaa tttatcaggt gataaatctt
    10021 tatttggtgg gattcaaaat ccatatttgg aacaaagtaa gtgggggtgg gcaatagacc
    10081 ctataggttt aagatttacc ttgaactata tttatagaaa gtatcaatta cctatcttaa
    10141 ttacagaaaa tggtttaggg gcatatgata aaatcgaaga agatggaact ataaatgatg
    10201 attatagaat tgaatattta agtaagcatc tatctcaaat gaagtctgca attgaagagg
    10261 ataatgttga atgttttgga tatttgatgt ggggaccaat tgatttagtc agtgcaacta
    10321 caggtcagat gagtaagaga tatggattta tctatgttga cttagatgat gaaggcaaag
    10381 gaagttttaa gagatataag aaaaaatcat ttgattggta caaagaagta ataaaatcta
    10441 atggagaagt tttagatttt tctacggata tagtagtagg gtaagaataa gataaataga
    10501 agttaggaac taatgttaat tagtgtctag taaagcattt aaaatagagt atagggtctt
    10561 taaagtaaag tgtgtattct tcttaaaagt aatggcaact aaaattacta taaaaaggag
    10621 aatacacatt ttttatgttt atacttaagt aaatatttag acatattcat agtataattt
    10681 actcattata taaaaatagt atgatttact cactatataa ctttataaat tttctggaaa
    10741 aatgtcagaa tatattgaca aagaaaagat ttaaaattat aatctatatt aagaatagtt
    10801 tgacaatata aaaacaagta tttttataaa tagtgaaatt atttttattg taaatttaag
    10861 aataatttag gactactata aagataaagg tatagatgtc attatatgat aaatcatact
    10921 tgtatagaaa gaattttaaa gtatggctta tcattgattt aatataataa ataatttttg
    10981 tttctagtaa tagttcctag gaataagata tctaaattcg tttagaaata gtctaattta
    11041 caaaggaggg gatgcactaa tagataaaat ttctatataa agaagactta aagtgaagta
    11101 tattctgata atattggttt aatgggtata ttattatttc gttttagata ttttaagaat
    11161 aatttaagac gtttataaaa atgtattcta tatctaatat ttaaataaat ataagtcttc
    11221 caacatgtat actaatatag agagtagttg aagtacaatt ctaactcgta aaattatgta
    11281 gaaagtaaaa atcaaaaaga aatctagtaa gttgataact atagttaata tttagagagt
    11341 taactctagt tatatttatt tagatatttt aggaataatt taggacgtat ataatatcat
    11401 caaaaaatat caagctatac catatgtagt taagaataac tgcataatat acattttata
    11461 attattgttt tattgataaa aaattattat atttaggagg aaacaacaat ggaaaatttt
    11521 tggaatccac ttacttgctt tactgtatgt gtaatgattt atgcttttag tgagttttta
    11581 tctaagaaaa caaaaggtgc aatttcaaca ttgttatttg tatgtattat atttttacta
    11641 ggattttgga caaatatttt accaaaagac attacaggag catcagggat attagctgca
    11701 atgagcaact ttggtatagc tttattaata actaacttag gaacacttat aaatcttgaa
    11761 gacttatgtc atgaatggaa aacggttgta attgcattag taggtattgt aggaataggg
    11821 gttggctgtt taacagtggg tagtatgtta tttggtagag agtatgcttt aatagcagca
    11881 cctccaatag cgggttctac tgttgctggt attatcgtaa caactgttgc agaagctgca
    11941 aatagacctg aacttgctgc atttgtaatt ctagtattat catttcaaaa gttctttggc
    12001 attccgataa ctacatattg tataaataaa gaattaaaat ttaaaagaga taatggggat
    12061 tttttgaaag atgagtcaaa aagtaatttt aaacttccaa gtatgagaat ttttaaagaa
    12121 gatttaggta aaaaatcatc tactttagtg tatcttgcaa agctttcatt tgttgctatg
    12181 attggtaatt ttgtaggatt ggctacttta ataccaggat ttacccctgc aaactattat
    12241 ttaaatccta acatagcatg tttgttagta gggttgattt ttacacgaat aggtttttta
    12301 gaaaaaggaa ttttagataa atctcaatct aatggaatta taatgtttgg ttgtatgttg
    12361 atgttacctg gaggacttgc taaggtaact ccaacagcat tgtttgggat gattgttcca
    12421 actataggaa tacttgttgt aggctctgta tttataatat tatttactgc aattgtagga
    12481 aaactattgg gatattctcc aagaatgtca gctgcaattg gtgtaacttg tatgctagct
    12541 tatcctgcaa cacagataat ttccaacgaa ggtattgatg ctatggaagg aactgaagaa
    12601 gaaaaacaaa gggcaacaga ttatttatta ccaaagatga ttataggagg atttgttact
    12661 gtaaccattg catcagtggc atttgctagt ataataggtc caattatatt tggttgatag
    12721 taaatatact tataggagga ataggaaatg ataggaagta tatctataaa tgagatagat
    12781 aaaaaaagat tcaaacttga tgatttaagt aagaaaatat gggaaaatcc agaaaaagca
    12841 tttaaagaat ttaaagcatg tgaaaataca gcaaactttc taagaagtga aggatttgat
    12901 gtggaagtag gtgtaggagg tttagccacg gctattagag ctagttttgg ttcaggtaaa
    12961 ccagttatag gttttatggg agagtttgat gcattacctg gtttaaatca aaaagtttca
    13021 acaaagcaag aagcatttga attgggtgca tatggtcatg gatgtgggca taatttatta
    13081 tgtacagcac atgtaggggc agtagtaggg cttaaaagag agatgataga aaataactta
    13141 agtgggacta tagtatttta tgcatgtcca ggagaagaac aattaacagg taaaggattt
    13201 atggctagag gtggagcgtt tgaagggtta gacttagcta taaattttca tccaaataaa
    13261 attagtgaag ctacaattgg tacttcaaca gctgttaatt ctgtaaaatt tcattttaag
    13321 ggaaagactg cacatgcagg gtcagaccct caaaatggaa gaagtgcact agatgctgtg
    13381 gaacttacta atgtgggagc taattatttg agagaacatg ttacttctga tgtaaggatt
    13441 cattatacaa ttacagatgg aggagtagca cccaacatag ttcctgataa agcatcagta
    13501 tggtattata caagagcttt aagtagagaa gctgtagaaa gtacatatga aagacttgta
    13561 aaggtagcaa aaggagctgc aatgatgact gaaactgaag ttgaagtaga gtttttagga
    13621 ggatgttata atactttaaa caatcatgtt ttagcaaatc ttgtatcaga atgtatgaat
    13681 gagattaaac aagagccttg gactcgagag gaattggatt ttgcagctga attagataga
    13741 caaagcccac aacaatataa ggctatgata agtaaatata atttgccaga aggaactcat
    13801 ctatattatg gtggaggaaa tgttacaaac tttaatagtt atggctctac tgatattgga
    13861 gatgtaatgc atatagttcc gacagcatac ttttttacag ggtgtacgaa tctaggagca
    13921 ccaggtcata gctggcagtt tacatcttgt gcaggaagtt ctataggaga aaaaggtatg
    13981 atatatgcat caaagattat ggctatgttt ggagctaaaa tattaaataa tccagagata
    14041 gctaaaaagg caaaagaaga atttgataaa tctatgaatg gtaaaacata taaatgtcct
    14101 attccagatg atattccaac tccttaaaaa gacatgaaat ttaatatata aaattataat
    14161 ttttatatca gcatcatttc attactaaaa gaaagtgaga ttttgctgac tatctgatta
    14221 ggaggaagga atatgaaaaa tttttttaaa gaagcgcagt caatagaagg ggaaattatt
    14281 agatggagaa ggtatttaca tgaaaatccc gaaattggtt gcaatctacc gaaaacagta
    14341 acctttgtta ccagtacttt agaaaaaatg ggatattctc cgaaattgac gtctaataat
    14401 ggaatagtag ctgttttaga cagtgaaaat ggtggaaaga ccttattatt aagggcagat
    14461 atggatgcac ttcctattat ggaggagacg gatttacctt ttaaaagtaa gaataaagat
    14521 gcatctcata gttgtggaca cgataatcac acagcaatgc tcttaggagc agctaaaata
    14581 cttatggata ataagcactt acttaaaggt agagtaaagt ttgtatttga accagatgaa
    14641 gagactacca gaggtgcaaa atcaatgatt gatgatggta tattagaaaa tcctcaggtc
    14701 gatgcagcta tggcatttca cagtatggtg ggtaaatttc ttaacacagg tcaagtagct
    14761 tattcacgtg gtccagctat ggcatctgcg gatatatttg aaataacagt agaaggaaaa
    14821 gcaactcatg gagcaagccc tgaatttgga gtggacccaa taaacataat gtcgcatata
    14881 caaatagcat taaacactat actttcaaga gaaaaacctc agaaagaacc tgtagtttta
    14941 actattggta tgtttaatgc aggaagcgta ccaaatgcta taccaaataa agcatctatg
    15001 tcaggtactt taagaacatt tgaccaattt gttagagaaa aattaaaaaa tagaattatt
    15061 gaaatatcta caggaatagc taaatcattt ggaggaaatg ctaaagtgga atttaaaatg
    15121 gggactcctg cagttataaa tgatttggat gtaggagatg agattgtatc ttatgttaaa
    15181 gaagttatag gaaatgaaaa tgtaatagac tttccatctg caatgggttc agaagatttt
    15241 tcagagattt tactaagggt tccaggagta tttttatgga ttggaatggg aagtattagt
    15301 cagggatata attttggtat gcataatcct aaagcaattt ttaatgaaga gggattagtt
    15361 tatggttcag ctttatttgc tcattgtgca gtaaaatggt tagaaaataa ttttagtaga
    15421 taataacata aaatattatt aaatattata aaaggtatat cttaaaaaga tgcaaagata
    15481 taccttttag aaagtcatat ataattttac ttaatattag atgataaaat atttttatgc
    15541 attttggatt ttaatattta ttttctgata taaataattt gtatactctt tcaggtctac
    15601 cttttgttga gttttgtttt tcatataata tagtagcgtt tccagacttt aagagactac
    15661 ttagaaatct attagcagtt ctaactgtta catttaaaca tgaagataat tccttaggag
    15721 taacttcatc tctttgtaga atttcagtaa cagatataat cttttgaata gtaagagtag
    15781 acaatttaga acttttggca attgtattta tatgtggagt tactttattt gaaacagtaa
    15841 gtaacttatc acacagtaaa ggtcctattg tctcataatt ttcgttaact aagaaactgt
    15901 aatttaaatg atttatttta gattccttat tagcacttat ggcactagac atagcttgct
    15961 ttatatcatt accaattcca tatccaacac atactttaat atttgtacta ttttcaatag
    16021 attcttttat ataacatgca gtaaaatttt tagttaatga tttaatcgtt tcacatttag
    16081 tgaaaatttt aaaagaatta ctattttttt caattataaa atcggttaat ttatcttttt
    16141 taatactatt taatatttta taaacttggt ctagagtaga gtcatcataa tttgtatctt
    16201 taactgttat atatattaca cttggttgat ttcccttcat tctatgtaaa tgaatatctt
    16261 ttattaagtt attaaaagta ttttttaata aatctatatc agggtatatg aaatgaaagt
    16321 ttactcctgc attttgtaac ataggaacaa tactactaaa acgagtagta gaaaaatcga
    16381 ttttcccttc attccataga ttaatatgtt tttttgcaat ctttttttca agttttgcta
    16441 tgtcagataa actcatttta gatgcatttt catcaatact atccattaag tctccaaatg
    16501 tgccatttga taaataatat gatatatctt ttggattatc tgcccagtct gttaagtcaa
    16561 agtatgctct agaaaaatct aatgtattat atttaaagat aagctttaga aacatatcat
    16621 agtaactttg taagtcagta ccaaaatcaa ctagaggttt tttaatagtt ccagcctttt
    16681 ttgtaatagc ttttttgggg atagggccac taactgcaaa tccatcaaac ctatcttcta
    16741 tttctaaata taaatctcca acatgagaaa aatctctata gatatatatt ttaatttctg
    16801 cattaatatt taactttttt atagaattat ctacaaactc ttttaaaaat tcggttgtta
    16861 tgactgctat tttcatcata gattacttcc t
//
LOCUS       DAELJO010000070        16848 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELJO010000070 DAELJO010000000
VERSION     DAELJO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.070"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            82..2985
                     /locus_tag="KPB88_001963"
     CDS             82..2985
                     /locus_tag="KPB88_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054243.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEAEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3018..3464
                     /locus_tag="KPB88_001964"
     CDS             3018..3464
                     /locus_tag="KPB88_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054244.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLYFIEI"
     gene            3587..3889
                     /locus_tag="KPB88_001965"
     CDS             3587..3889
                     /locus_tag="KPB88_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054245.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4070..5476
                     /locus_tag="KPB88_001966"
     CDS             4070..5476
                     /locus_tag="KPB88_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0054246.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5820..6878
                     /locus_tag="KPB88_001967"
     CDS             5820..6878
                     /locus_tag="KPB88_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0054247.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            6977..7663
                     /gene="araD"
                     /locus_tag="KPB88_001968"
     CDS             6977..7663
                     /gene="araD"
                     /locus_tag="KPB88_001968"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG0054248.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7681..8328
                     /locus_tag="KPB88_001969"
     CDS             7681..8328
                     /locus_tag="KPB88_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0054249.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8501..9868)
                     /locus_tag="KPB88_001970"
     CDS             complement(8501..9868)
                     /locus_tag="KPB88_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG0054250.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10058..10146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10574..12700
                     /locus_tag="KPB88_001971"
     CDS             10574..12700
                     /locus_tag="KPB88_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG0054251.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYRDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQNQETFNNPFAEALAKLKNK"
     gene            12804..14057
                     /locus_tag="KPB88_001972"
     CDS             12804..14057
                     /locus_tag="KPB88_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0054252.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14211..15272)
                     /gene="ytvI"
                     /locus_tag="KPB88_001973"
     CDS             complement(14211..15272)
                     /gene="ytvI"
                     /locus_tag="KPB88_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0054253.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15546..16286
                     /locus_tag="KPB88_001974"
     CDS             15546..16286
                     /locus_tag="KPB88_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG0054254.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16298..16507
                     /locus_tag="KPB88_001975"
                     /pseudo
     CDS             16298..16507
                     /locus_tag="KPB88_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6743 a         1880 c         2925 g         5300 t
ORIGIN      
        1 agggtttaag tagaaaaaaa tatcaaagta agtatggcat gatagttgct tatatattta
       61 tcaagaaagg agttattaag catggagttg agagataaac ttgtaaacat cataaataca
      121 gaaaataaaa aagatccatt aacagatgtt caaatagcaa agtttttaag tacaactaga
      181 gagaacataa ctaatttgag aaaagaactt aatataagta attcaaggca aagaagatat
      241 ccttatttaa aatcagcaat ttcggcaata ttacaaaaga ataagaatat ttctatatca
      301 gaaattacaa gggaattgat gacagaaggc tttaatatat cgagaagagt tgtggaggaa
      361 cttttaccta aagaaagttt agaaatggat gtagtagagg aagctgaaga agaatcgaat
      421 gacccatttg aaactctgat aggtaataaa gggagtctta gaaatgctgt agagcaggct
      481 aaatctgcta tattgtatcc accaaaaggg ttaccaacgt tgattattgg ggaaagtgga
      541 gttggtaaga gtttattttc tagacatatg tatgagtttg caaagcaaaa aaagatagta
      601 aaagagagct ccaattttgt tgtatttaac tgtgctgatt atagtgacaa tccacaatta
      661 ttattatctt tgttatttgg atataaaaaa ggtgcattta caggtgctga atatgataca
      721 ccaggtcttg tagaagaagc tgatgaagga gttttatttt tggatgaaat acatagattg
      781 cctccaaaag gtcaagaaat actattttct attttagata ggggaaagtt taggagatta
      841 ggtgaaacta atgctgaaag aaaagtaagt attatgttga ttggggcaac tactgaaaat
      901 gtagagacta atttattatt aacattcaga agaaggatac caatgctaat aactttgcca
      961 ccattacatg atagattact aaaagagaaa atagacctta tatacaatat ctttcaacaa
     1021 gagtgtaata gaataaatgc aaaaatattt gtagataaaa atgtgattga aatacttgca
     1081 ctaaaaaagt ttagtggaaa tataggacaa ttacaaaata tgattcaggt tctttgtgca
     1141 agagctttta tgaagtttat aaattccaaa gatgaagata gtgaatcgat agtagtagta
     1201 gatataaatg aagttttaaa gcttaaagat tcttttaaag atgctgcctt tcaagagatt
     1261 gaatatacag agataagaaa atatttgaaa aatgctatat ttataccatt taatttagaa
     1321 gaatcatcag taaaaggaag tcttttaagt gatgaatata tattaccaga ggacatatac
     1381 aaaacaattg aaaagaaata ttatgaccta aaatccctag acattgaaga tatagatata
     1441 gagaatattt tatggacttt tattttaaat agatttgctg acttaaagtt taatctcgat
     1501 agtaatgaag aaattttttc aatgagtgat ttaaatagtt ttgtaagtga tagtttagtt
     1561 aaactggtaa aagaacttat gaatgatata gttgaaaaaa atatgaattt agaagtaaat
     1621 aaaaatatgt tcaaatatct agcaatacac ttagaagaag ctataaaaag aattaagcta
     1681 aatcaaaaga taataaatgt aaatttagaa aaaataaaga ttgacttttc aaaagaatat
     1741 gaaattagta aaagttttgc aataaaaatt gaggaatcaa ttggtattaa gattccagat
     1801 gatgaaattg gatttataac actctacata aagtctgctc taaaaaatga agtgaaaaaa
     1861 gataaagtag ggttaataat aatttctcat ggacgtatag ctacagagac tgtaaatgta
     1921 gttaaagaac ttttaggtgt aaagtttcct gtggctatag atatgccttt agatgaaaaa
     1981 ccaataaata tatacaataa ggcagtagaa ctgtcaaaaa taatagacca agggaaaggc
     2041 atattgtttt tggtggatat tggttcactt acaaacatag gtcaaatagt aaataagaga
     2101 accaatataa gcactaaaac tattgataga gtagacctcc taatggcact agaagcaact
     2161 agaaaagttt ctatgggaga ggaagaactt gatgaaatat ttttttcttt gctaaaagat
     2221 aggatgggat ataactatga attagaaaaa cacatagata aatcaaatgc aattgtaaca
     2281 ttatgtctta ctggagaagg aactgctaag tatataagta aaacgttgga agaaaagtat
     2341 gataacacta aatgttatca aatgagtgca ttggatgaaa atttattttg taaaattgaa
     2401 aatctaaaag agacaaacaa tatattagct attgttggga caataaaccc taagatacca
     2461 ggtataaatt ttatacccta taataaagaa cttttcaaaa atttggatat ctatctatca
     2521 aaatcaaaac aacaagatga cagaccagtt aaatcgtatg aaagaatgct tgatgaggat
     2581 ttagtgattt ttgaaccaga tatatatttt aaaaaggatt tattagaata tgtatgttca
     2641 attcttataa ataaagaata tgtagaaaaa gattacttag attctgtttt acatagagaa
     2701 gaaatgcttc caacatattc taaaggagct ataggtgtgc ctcatggaga ttcttctact
     2761 gtaaatagta caaggtttgt ttttgttaaa ctaaagaatc ctattgattg gggtgtaggt
     2821 aatgtaaatt tcatatttat gccagttttt caggctaatg ataaagagat tgttaaaaat
     2881 gttcttgaga tactaaaaga tgcagaattt atgaacaatg ccaataagtg ttttgataaa
     2941 gatgctttta gaaggattat atttgataaa tttaaacatc tgtagccata gcaaaaaaat
     3001 tgagaggagt tagagtaatg aatgtagatt taatatcatt gggtttagaa tctaatgatt
     3061 atgaaagtgt tattgaagaa ttaggaagta taatgtgtaa aaaagaatat gtaaaagaaa
     3121 catacataaa tgctgtcttg gaaagagaaa ggacattacc aacaggtcta gatattggag
     3181 aaatgtgtgt tgctattcca catacagatt caaagcatgt aaatgagtct aatgtagctg
     3241 tagggatatt gaaaaatcct gtaaagttta attcaatgat agacccaaaa gatagacttg
     3301 atgtagaact tgtattttta ttagcagtaa agaacccaga ttcacaagta aaactgttaa
     3361 aggatttaat gtcagttttt caaaatataa agttattaaa aaatataaaa aacgcatcaa
     3421 caaaagaaga agtagctaaa ttattgtact ttatagaaat ttaatgtagg agaattttat
     3481 aaataaaaaa ttaaaattta aacaatcaaa gtaagtatta tttttttaat aggtttaatc
     3541 ttatataaaa taaaaattta gagtgaaaaa ttaggaggaa ataattatga aacaatttaa
     3601 tgtattatca gtttgtggtt cagggacagt tacatcatct atggtagcag gtaaattaaa
     3661 agaagtacta ggtaataagg ggatttctat tactacaaca gaagctagac caacagaagc
     3721 actaaattta gcacaatcag gaagatttga ctttataaca tttacgagtc cacttcaaac
     3781 aggcgattat ggaataccaa ctataaatgc atttgcttgc ctaactggta taggggaaga
     3841 ggcattcttt gaagaagttt tgactgttat tgaagggata gataagtaag ttttggagac
     3901 gtgaatttat agtatttaaa tcaatgactt agatactata ataagctaat attaactaaa
     3961 tatttgtgga aaaaacattt tatatgttgt cataaattaa aaaattataa aaatagtcac
     4021 ttaagtatta aattaagttt ataaatattt ataagtagga gggaaaaaga tgttagaatt
     4081 tatgaaatct ataatcgata catttggttc tgcaattata gttccaatta taatattcat
     4141 aatcgcaaag atatttaaag taactactaa gaaatcgttt ctttctgctg tgtatgcagg
     4201 agttgcttta caaggtttta cactgatttt aaattcattt acaccaataa taacaccagt
     4261 tatcaatagg atggttgaga gtacaggagt aaacctacca gtttttgatg ttggttggca
     4321 agcaacatca ttggttgcat tttcaacaag tgcaggtatg atatatttag gtcttggtat
     4381 attattacaa acaattttat ttttgataaa atggacagac gtgtttcaac catcagattt
     4441 atggaataac tattcttata tggtatgggg agcaatggtt ataggagtaa caggaaattt
     4501 tcctttaggt atagcatgta tggttttatt aaacctatat agtttattaa tatcagagtt
     4561 agttgcaaag agatggtcta gctactatag atatcctaat tgtacaataa tagctatgca
     4621 taatgttgaa gcttctgtgt ttgcagtatt tgcagaccct atatacaata aattgggatt
     4681 aaataaaata aaattaaatc caaaagaatt agagaagaaa ttagggtttt taggtgaacc
     4741 aattacttta ggtctttttt taggtatgtt tataggtata ttaggtaata tgacgagaat
     4801 aaatactatg gaagcttggg gagaaataat gaaagtaggt atatctacaa gtgcagtgat
     4861 ggcaattttc cctaaagtag caagtatgtt tgctcaagca tttgcaccta taacagaagc
     4921 agctagaaag attatgcaaa aagctggaaa ccgtgagtgg tacatagcag taaatgatgc
     4981 tgttggatat ggtgaacctg caactttaat atcaggtctt atattaattc caataatgct
     5041 tgtaatagct atggtacttc ctggtaataa ggtattacca gttgtggatt tattggcaat
     5101 tccatatatg gttcaaggtt tagttgccat acataatggt aatataccaa aagtattagt
     5161 ttcaggtata atttggtttg gattaggatt atatgtttgt acatctacag caccattatt
     5221 tacagatatg gctacaaata taggtgtagc tatacctgca ggagcaatgt taataacaag
     5281 ttttaatata ctaggaaaac ctttgatggg gttagttttc tttgcatttc taagtgcaaa
     5341 tccaatttat ataggtcttt cagttgtaat ttactttgtt ctatgggcat tgtttagaaa
     5401 gaacaaaaca agtattcttg attatttaga aaaacaagca cttaaaaatg tagaagaaga
     5461 gcctgtagca gtttaagttt ataaaaatga aataagaata attaaaggag tttataattg
     5521 attttatata agtatcagat aatacctgag aaatatttaa atgaaatata gacttctttt
     5581 tatttgataa tatttgtaat tataatgcat tgtattttta tagttgagtt ttctgtataa
     5641 ccttctattt aaaacatact cttttatatt tatacagtaa tgtggtttaa ttttataaaa
     5701 tgtaatctct actggtaaaa gactttgtat ataaggcatt tgcctgaata aaccatttat
     5761 cttttgtatg gcatagatat tgctatatag aagtatatag catgaaagga gatatttata
     5821 tgggaaataa aatgagagca tcagtattgt ataatgtagg tgatgtcaga tatgaaatgg
     5881 tagatatacc agaaataaca gatacgcaag tgcttgtaaa tgtaaaatat gttgggatat
     5941 gtggttcaga tttacctagg tctatggtaa gtggtttgag tggaaataca aaatatccat
     6001 tgattttagg gcatgaattt tctggtgaag ttgttaaaat tggagaaaaa gtaaagcata
     6061 ttaatgtagg agatagagta gctgttgcac cattagtacc ttgtggaaaa tgtgattatt
     6121 gtaatgaagg taattttggc ttgtgtgatg attacaatat aattggaaca agagtaaatg
     6181 gagcgtttgc agaatatgta agagttccag aagaacatat tttaaaacta ccagatacat
     6241 tggattatga aactgctgcc ggaattgaac cagcaactat agcttatcat gggatttcaa
     6301 agtctaatat tagagtagga gatagtgtag ttgtgttagg atgtggtcca ataggtcagt
     6361 ttgttataca atgggcaaag gtgtttggtg catctaagat tatagcagtt gatatttttg
     6421 atgaaaaatt agaactttct aaacttctag gagcaaacta tatacttaac tcaaaagaag
     6481 taaatgtaat caaagaaata aaaaaaataa caaatggtgg tgctgatgtt gtaatagaga
     6541 ctgctggtag ccgatttaca caagaacaat ctctatttgt agcaaaaaag agaggaaata
     6601 tagtgtttgt aggtatatca cacacagaat taccacttag tgcagacgct actgagtgta
     6661 tattacgtgg agaacttacc ttgaaaggtt cttggaattc atatacaagc ccttatcctg
     6721 gaagagcatg gactgcaaca ttggatttta tgggaaaagg tgacattatt tttaaaccca
     6781 tgatttcaca taaaataggt cttaatgaag tgggtgactt tttaagtaaa atgagcaaga
     6841 gagaaattaa tttcaataaa atcctagtag aaatataaaa atttaataaa aaattctaaa
     6901 agcaattaaa ttttataact atgacattaa ataaaataaa aataattttc ttaaataaaa
     6961 tataggagga agaaaagtgt tagaaaattt aaaagaagaa gtattaaaag caaacttaga
     7021 attacctaag aaaaagctaa ttacttatac ttggggaaat gtaagtggga tagatagaga
     7081 aaaaggatta gtggtgataa aaccaagtgg tgtagagtat agtaatatga ctgtagaaga
     7141 tatggttgta gttgatttag agggaaatat agttgatggc aaattaagac cttcttcaga
     7201 tactccgact catctagttt tatataaaga gtttgaagaa ttggggggaa tagtacatac
     7261 acattcaagt tgggcaacaa tttgggctca aataggaaaa tctataccat cttgtggtac
     7321 tactcatgca gattattttt atggaagtat accatgtacc agaaaaatga ctagagaaga
     7381 aattcgcaat gaatacgaaa aggaaactgg aaatgtaatc attgaagaat ttatgggaag
     7441 aaatccaatt cattgtccag gaataattgt aaatgaccac ggacctttta catggggaaa
     7501 agatgcaaat gaggctgtac acaatgcagt agttttagaa gaagtcgcaa agatggctta
     7561 ttatacagag ttaatgagtc ctgataatat tatggataag gttcttatga ataagcattt
     7621 ttcaagaaaa catggaaaaa atgcatacta tggtcaaaaa tgattatctg gaggtgaatt
     7681 atgctagact ctttaaaagg taaagttgtg gaaatagcaa ttaaagcaga gcattatggt
     7741 ttatgtaaag aaaagactgg taattttagt attagagata aggatactgg atatatactt
     7801 ataaccccat caggtgtagg aagagaagaa ttaaaagttg aacatatttg tgtattggat
     7861 ttggatggaa atgtaataga agttgagaaa ggtattaagc caagtagtga ggtattgatg
     7921 catattgaaa tatacaaaac aagagaaaat ataaatgcca tatcacatac tcattcttta
     7981 tatgcaacag cattttcagt agcaaagaaa ccaattatac caattgtata tgaatcagtt
     8041 aattatggcg gttatgtaga tattgctcca tacgaaagac caggaacttt aaaattagct
     8101 aagagtgttt caaaattact gatagataca gatgcatgct tattagaaag acatgggttg
     8161 acaactgtag gtaatgattt agacgaagca ttattaaaat caagatatgt ggaagaggta
     8221 gcagaaatat actataggtc tttagtatta aatcaattta aagaacttga agtagtagat
     8281 gttgatgagt ttaaatcttg gaaataccca gaagaaataa agttttaatc taaataattt
     8341 tcattgaagt ggttaattaa aatatatgta ttatttatat caattactat gttttattat
     8401 gttaattacc gtattttgca aatatattta ttacattttt tcaaaaaaat atagaatccg
     8461 aaagccatat aaaattgact tgggattcta tactttttat ttatgcattt ttctttatct
     8521 gattttctgg agaatttata tgttcaaaat attttttagt ttcagatact attataggtg
     8581 ataatgcaag taaagctata aggttaggta ttgccataag cccatttaca atatcagcaa
     8641 ttatccaaac aacttctaac tttaagaaag cacctaataa caccatagcc acaaatacag
     8701 ttctataccc attcatgcct tttacaccaa acagaaattc aaaacatctt tctccataat
     8761 aactccatcc caaaattgta gtaaatgcaa ataatgttaa actaactgta agtaatattg
     8821 gtccaaatgt aggaattgct ccgttaaaag ctgcttgtgt cataacagct ccatttaggt
     8881 ctgaacacca cactcctgaa ataacaagag agaatccagt aagagtacag attataatag
     8941 tatctataaa agtacctgtc atagatataa gaccttgttc cgctggccat tcagttttag
     9001 cagcagcagc agctattgga gcactaccta gaccagactc atttgaaaat acaccacgag
     9061 cgataccatt tctaattgct agtgacatag ttgctcctaa aaaaccacca gtagcagcag
     9121 taggtgtaaa tgcactttta attattaaca taaaagtttc agggatttta ttccaaaaag
     9181 atattaaaat gattccacag attacaacat atataactgc cataaaaggt actattttag
     9241 tagctacatt agatatactt ttaagtcctc caaatacaac taaagctaca aatactgcta
     9301 atacaacccc tgtaattcta gggtctatcc cgaaactatt atttattgca tctgttattg
     9361 aatttacttg agtaaaagta ccgattccta aaagtgcaac tagtatacca cttatagcga
     9421 aaaatattgc aagaggtctc catttttctc ccataccatt tactatgtaa tacattggcc
     9481 caccagacac ttgaccattt ttatctttag ttctgtattt tattgcaaga acaccttctg
     9541 aatacttagt agccattcca aagaatgctg ctatccacat ccaaaaaagt gcaccaggtc
     9601 caccagcttt gatagctgta gcaacaccaa caatattacc agtaccaact gtagcagcta
     9661 gagcagtaca taaggctgca aaacttgaaa catcaccact acctttgttt tcagcagaga
     9721 atattaactt aagagctctt ggcagtttag ttatttgaac aactccaagc ttaacagtaa
     9781 gtagtatacc agtaccaact aatagtacca gtaggggtgg tccccaaata aaagcatcaa
     9841 ctttgtttaa aatatcaatt agattcataa aaacactccc tcattttttt tgctttacat
     9901 aatcaataga atttttaatg aagtaattga caggaaataa gtataattta ttaataattt
     9961 ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc tctggatgta cactaattag
    10021 caaccaaaat aaaagtatta gtaaatactg tttttggtag ataatccctg tccttttgcc
    10081 tgagagattg agcataaaag ccttgcccct tcggcgccca taatgggtct ctccagagtt
    10141 ccgtaaattt tacagtccta cttttaaaag tacctgagag tgttactcct tcggtgtgct
    10201 atatagcaac tcttctataa aatcttaacc ggatattgat ttgtcatagc ttattgtatt
    10261 aataatatta atataagttt ttatgttttg tcaactaaaa aattaaaaaa caaagaaaaa
    10321 atataaaaat gtaagcctat ttacgaatta taaacttaga aagatgagta atatacgtga
    10381 aaatgaattt tgtaatacac atacaaatga agtgtaaaat aagttttaaa taaaaaaata
    10441 ataaaatctt aaataattaa ttagatatga ttaaattatg gtaatattat agttacgata
    10501 ttaagaaaag ttctatttag ttagatttta agcgtaatat gagattatgt atagagaaag
    10561 gatgtatttt tagatgggaa aaatattagt gctagcagaa aaaccttctg ttggaagaga
    10621 tatagcaaaa gttttagggt caaaaaatga aaaaaatggt tatatagagg ggccaaagta
    10681 tgtgataact tgggcactag gtcatttggt tacacttgct gaccccgaaa gttatggaga
    10741 aagatataaa tcatggagct tagaagacct gccaatactt cccaagcacc ttaagactgt
    10801 tgtaataaaa aagagtggaa agcagtttaa tacagttaaa tcgcaaatga atagaaatga
    10861 tatagatgaa atagttatag caacagatgc aggtcgtgaa ggagaacttg tagcaaggtg
    10921 gataatagaa aaatcacagg taaaaaaacc tataaaacgt ctttggattt cttcaagtac
    10981 agacaaagca ataaaagacg gatttgcaaa attaaaaagt ggaaaagaat atgaaaattt
    11041 gtattactca gcaatagcac gagcagaagc tgattggatt attggaataa atgcaaccag
    11101 agctttgaca acaaagtaca atgcacagct ttcttgtgga agagtacaaa ctcctactct
    11161 tgcaatgttg cttaaaagag aagaagaaat aagaaatttc aaacctaaag agtattatgg
    11221 attagaatta attgctacaa agggaaattc agatataaaa tttatttgga atgataaaaa
    11281 caataattct tcaactttta gtaaagaaaa gatagaatcc acattaaaaa aagtaaaagg
    11341 tgtagacata aaaatatctg aagtaaacaa gtcatataag aaaaaatatt cacctgcact
    11401 ctatgattta acagaattac aaagagatgc aaataaaatg tttggatttt cagcaaaaga
    11461 aactttatct ataatgcaaa aactgtatga acatcataaa gtacttactt atcctagaac
    11521 tgattctaga tatttaacat cagatattgt tggaacatta aaagatagaa taaaagctgt
    11581 aaatattagt gattattcaa agatttgtaa tagattatta aaaagcaata ttatagctaa
    11641 taaatcgttt gttgacaatt caaaggtaac agaccaccat gcaataatac caacagaaca
    11701 gaaggtattc atgagtgatt ttagtgataa agagcgaaaa atatataatc ttgtagtaaa
    11761 aagattttta gcagtactat ctcctccttt tgaatatgag cagacaacta taagtgcaac
    11821 tgtagaagga gaaatattta aggctaaggg aaacaaagta ataaatttag gttggaaaga
    11881 aagttataga gatattgaag atgaagatga gtatgaaagt ataccaaatg ttttagaaaa
    11941 agatgtactt aaggtcaagg gattaaagat aacttcagga aaaacaaatc caccaccata
    12001 cttaaatgag ggaacattat tgactgctat ggaaaagaat ggtctaggga cagttgctac
    12061 aagagcagat ataatagaaa aattattcaa ttcattttta atggaaaaga aaaataaaga
    12121 gatatatata acatcaaaag gaagacaatt attagattta gttccagtag acttaaagac
    12181 accagaactt acagcttcat gggaaaagaa attattggat atttcaaagg gaaaacttag
    12241 taaaaataca ttcataaatg acatgaaaga ttattcaaaa gatattgtac tagaagtaaa
    12301 aaatagtgat aataaattta aacatgataa cttaactaaa aatagatgtc cagaatgtgg
    12361 aaaatttatg ttagaggtaa atggtaaaaa aggtaagatg ttgatttgtg aagatagaga
    12421 atgtaatact agaaaaacta tatcacaagt aaccaattca agatgtccta actgtcataa
    12481 aaaattagaa cttcgtggag aaggtgaggg gagaatcttc acttgtagtt gcggatacag
    12541 agaaaagttg tcgtctttca acaagagaaa gcaagaagaa aaacagaagg catcaaaaaa
    12601 agatgttaat caatatttaa aaaatcaaaa taaaaatcaa aatcaagaga catttaataa
    12661 tccatttgca gaagcattag ctaagctaaa aaacaaataa ttttaaagtt attattatac
    12721 acaattataa ctttaactgt atattagtta tatgtactga tataatatag tgtatagttt
    12781 aggcatgaaa gtagggctgt taaatggatt taagtggaat atttaaatac tattgtaaag
    12841 agtgtgaaaa tacatggaat aattcgagtg ttgaattatt tgagaatata gaaacgtata
    12901 gtaaagattc acaaaaaaag agggaaaaag aattagataa attgctaaat acaatatcag
    12961 ttcatttaga gaggtatcca agtgatgctg tattgagaaa aatgtgggta aaaaagggcg
    13021 aggttttctt acaaaagaca ttggaaaaag aaaatatttt taagttagaa aaaatggatg
    13081 tagaggatag aaaaaaattt ttagatataa caaaacagtt tattagagat gctagaaaat
    13141 ttgatgatga tttacctata ggtgatatta tgcaagctat gaggaatgta tggatttcaa
    13201 atgcattaca attattattt ggtaaagaag tatattattc aaaagctaac tttgcatata
    13261 gtatgttata tccatatact gataattatt tagacaatac aaatatagat aaaaatgata
    13321 agattttatt taataactgg ttagaaaaaa ggctcctggg agaacacatt aaatctaagg
    13381 attatcatga aagtaaagta tctcaaatga tagattatat tgaaagtgta taccctagag
    13441 aaaagtttac agaagtttat gaatcgttat tattaatatt taaaagtcaa gtaaatagtt
    13501 taaaacaaca tggtaaggaa aatcatttgt gtaaagaaga tttattatcc atttctattg
    13561 aaaaaggagg ttcatccgtt ttagtagatg gatatttaat aagtggattg atgacaaagg
    13621 aagaaataga gttttgtata ggatatggat ttttattaca aatatctgat gatttacaag
    13681 atataaaaga agatttaaaa tacaaccata aaactattat tacagagatg tcaaaagagg
    13741 gtactttaga taaagttgta aataaactaa taaattttac tattgagtta atagatagtt
    13801 ttaaaattaa taataaaaat aaatctgtaa taactatgat aaagaatgat tgcttaatgt
    13861 taattttatt ttctgtagtt tataatgctg aatttttttc tgtaggatat ataaaagaag
    13921 tagagaaatt tattccatat acaatagatt attcattaga gattgaagaa aaaataaaag
    13981 aaaaatttaa aaatatagat gttttaaata atgaaaatga atataaagaa atgattgata
    14041 ttatttgtgc agagtagaaa aaaagggagt gtcttagaat agacttttta gtttatgagg
    14101 cactcttttt gtataaaatc aaatgtattt tcatcatttt aacaattaaa aagagcttat
    14161 ccctatttga gaaagccctt tttaatttaa ataaaaatgt caaggttaaa ctatttaaat
    14221 gcagtaataa tcccattatc atgtaagttt tttatgattg tagctgcgat agggaatcca
    14281 aataatccag ctattccaaa tagttgagcc ccaacaaaca tacacattaa tgttattaaa
    14341 ggatataggc caatttggtg tcctactacc tttggttcta atatttgtct aacaacggtt
    14401 ataattatgt atagtattaa aagtcctatt gctaaattaa tatttccatt aatgagtgag
    14461 ataatcatcc aaggtgtcaa aatacttcct gtacccaaga ctggcaaaat atcaacaagt
    14521 gctatgatga cagatatagt aaatgcattt tcaaccttta ggatagtaag tccaataagt
    14581 agttctatga atgtcactga tagtagtatt gcataggctt ttagtagttt taaaagagca
    14641 ttaatactgc tattttttat atttattatc ataaattgac ttttttctgg aaattgtttt
    14701 aagataaagt ttactatttt ttgatagtca aaagtaaaga aaaaagaagc aattaaagta
    14761 ataataagtt ttaacataaa tgcaggtgtt ttacttgcaa tactagcaat agcattgagt
    14821 gcatttgttg aaatagaagc tactaaagac atcatagatt gacttatatt atcccaacca
    14881 agtattaatg ctaggttaac attaggtgta ctagcatcta tctttgaaaa taaagtattt
    14941 agcgttggtt gtatacttga tttatatatt tcaggaagtc taaaaaatac atcacttaca
    15001 cttgcaaata tcttcacacc aaagctaact aataaaaata tacacaaccc ataaaaaatt
    15061 aaaagtatta ctatagaaat aagtgtacga tttaaatttg tattatttgt aataagtctt
    15121 ataataggtc ttaagatgga tgcaatgata aaactgatta caaaaggcat taataatggc
    15181 aataagaatt ttaatacaat atatacaata cttgttatta aaactacata tgtaaattta
    15241 attataaaat tcttttgctt ttgaatatcc ataatataaa acctccaatg tatttgatat
    15301 tattctaaat atggtctttt ttacagaaaa tatactactg tatattgatt ataaactata
    15361 aagcttatgt aaagtaaata aattatgtaa aatttaggtt aattttgtat aaagagatac
    15421 ttttatattt tacaattaat aaccaaatac taaaaaatat tttaaataaa gaacttaaaa
    15481 ttgtgataaa attaatttat agatattaaa aaaagtataa tatatggttt ttaagaggtg
    15541 ctaagatgaa aaattttaga gatttaggtg ggaacaaaac tgaggatggc agaactgtaa
    15601 agaagggatt attttatagg tctgcaaaat tatcaaatct aagtgaaaat gatataaaga
    15661 ttttaaaaga gttaaatatt aaatatatat ttgattatag aagtgatgaa gaagctcgta
    15721 aacatccatc aacaataata tcaaatataa aaaatataag aataccagca atgagagagc
    15781 tagaagaatc aggaggaagt tttggttcta ttgaagatat gattgatgga ttgtttgaaa
    15841 aagatggtgc atttaatatg ttaaataata gttactataa tttgcctata aataatccgt
    15901 catataaaaa attagtagaa cttataagag actattctaa tttaccaata ttaaatcatt
    15961 gtacagctgg taaggataga actggtgtgg ggagtgctat aattcttatg attttagggg
    16021 tatcgagaga gaatataatg aaagattatc taaaaagtaa tgattttgca gataaagaga
    16081 tagaaagatt tatagaatat aagcctaaat ttaaggacat tccaaaagaa aatttgaaat
    16141 atatatttgg tgtaaatgaa gagtatatga aaactgcatt tagaagaatt gatgaagaat
    16201 acattagtgt agaagcatat ttatatggag aatttaattt aaataaagaa gaaataagaa
    16261 aattaagaaa tcaatatcta gagtaaaagg tgagtttatg agtttaaata taacagcaag
    16321 acagataaat ataataaaaa tattgttaaa ttcaaaagat acaattagtg gtattgcctt
    16381 aagtcaagag ataggatgtt cttcaaaaac tatacaaaat gaaataaagg atattaataa
    16441 acaacttaga aatggtaaaa ttttgtcaat aagaggagtt ggttataaac tagaaggaag
    16501 ttttgattaa aaaatagcta cgcacacttg tgactttagt catgagttag tagctaaatt
    16561 agtcagcata tagggaaacc tatatgtaga gataggatag aacctatcca atactacttg
    16621 aattgctaaa aacccctaaa gctaaccaaa ctaaaacata aggatgaaat aaacctaagt
    16681 gtgaaagtta cgaaagtaga aaaaattggt tagatggtat aaggttaaat cctaagtact
    16741 ttaaaatggg caatcagcag gtaagctccg aataggagaa acttcaacga ctattcctct
    16801 tgagggaagt acactacaag ctattggtag tggaagtggg tagacctt
//
LOCUS       DAELJO010000071        16776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELJO010000071 DAELJO010000000
VERSION     DAELJO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.071"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            241..1053
                     /locus_tag="KPB88_001976"
     CDS             241..1053
                     /locus_tag="KPB88_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0054255.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSTTMGLVLQK"
     gene            2097..2858
                     /locus_tag="KPB88_001977"
     CDS             2097..2858
                     /locus_tag="KPB88_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0054256.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2923..3705
                     /locus_tag="KPB88_001978"
     CDS             2923..3705
                     /locus_tag="KPB88_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0054257.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            3972..4391
                     /locus_tag="KPB88_001979"
     CDS             3972..4391
                     /locus_tag="KPB88_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0054258.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4494..4760)
                     /locus_tag="KPB88_001980"
     CDS             complement(4494..4760)
                     /locus_tag="KPB88_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG0054259.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            4987..5688
                     /locus_tag="KPB88_001981"
     CDS             4987..5688
                     /locus_tag="KPB88_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054260.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5770..6378
                     /locus_tag="KPB88_001982"
     CDS             5770..6378
                     /locus_tag="KPB88_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0054261.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6702..7166
                     /locus_tag="KPB88_001983"
     CDS             6702..7166
                     /locus_tag="KPB88_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG0054262.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            7802..8818
                     /gene="gap"
                     /locus_tag="KPB88_001984"
     CDS             7802..8818
                     /gene="gap"
                     /locus_tag="KPB88_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0054263.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            9212..10381
                     /locus_tag="KPB88_001985"
     CDS             9212..10381
                     /locus_tag="KPB88_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054264.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIV
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            10762..15015
                     /locus_tag="KPB88_001986"
     CDS             10762..15015
                     /locus_tag="KPB88_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0054265.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATEEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            15164..15658
                     /locus_tag="KPB88_001987"
     CDS             15164..15658
                     /locus_tag="KPB88_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0054266.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            15774..16151
                     /locus_tag="KPB88_001988"
     CDS             15774..16151
                     /locus_tag="KPB88_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG0054267.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            16357..>16776
                     /locus_tag="KPB88_001989"
     CDS             16357..>16776
                     /locus_tag="KPB88_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG0054268.1"
                     /translation="MKNLNLLINKLYSKNHKEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDIND"
BASE COUNT         6833 a         1627 c         2917 g         5399 t
ORIGIN      
        1 tgatatttga tatcataaaa tcgttgatat aagtattgct ggaaaagagt gaataaaatc
       61 aaatttaata agcaaacaaa gtaaagctgt agttttattg ttgtgcttaa aaatatttgg
      121 ttctatttta ctcttattta aactcatctt tttgaagtta aaccacagag taaactctgt
      181 ggttttttta ttttaaaaat aaattttaag gagtggtatg ttttgttaaa caaattatgt
      241 atgtatttag aaagaccaga gctttataaa caaagtgaaa ttaatttttg ggatgatgag
      301 tatatttcaa agcaattatt aaaagcacac ttagacacta attttgaagg agcaagtaga
      361 aattttaact ttatcgagga ttctgtaaat tggatagtta cagttgcaaa tccagttaat
      421 tatcctaaat tacttgattt aggttgtggt ccaggtttat atgcagagaa atttgcacaa
      481 aaaggttata aggtaactgg aattgatttc tcaaaacgtt ctattaacta tgcacaaaat
      541 agaaataagg agactaattt aaacataaat tacttgtttc aaagttattt aaatatgaac
      601 tataatgagg aatttgattt ggctacttta atttattgtg attatggagc tttgtcaact
      661 gaaaatagaa ggttattaat ggaaaaaatt tatgatagtt taaaacctgg aggaaaatta
      721 atattagatg tatttacaat aaataaatac aataattttg aggaaattaa atattgggaa
      781 ataaatgaag atggaggatt ttggagtaat gaaaaatata tgtgtctaca agataattgt
      841 aaatacaatg attataatac attagaacaa actcttgtaa taaccgaaaa agatgaaaat
      901 gtattttatg tttggaatca ctatttttca aaagaaagtt tcttattaga agttgaaaat
      961 attggattta agtctgttga gttttttagt aatgttaagg gtgaagcata ttctgacgat
     1021 agtacgacta tgggattagt tttacaaaaa taataatttt ttttaaaatg aataaataaa
     1081 aacttgaaac ctttaacaat aaaataacat aagaaaagct tacatattga aaaatggaaa
     1141 cttttatgtt atttttagta cattacaaat ttgcttatat aaaaatcaat acttgaatta
     1201 ttatttttat gtgaaattta tctatagtat atttttataa acaagtaatg ttattaaaat
     1261 taagtatata gttaaaagtg tatagcaaaa aggctgttat attaatgtta gaggttcagg
     1321 gaaaatatat aaagcaaagt atttacagat aatgtagatt gcacagctat aagttagata
     1381 atagaattat gtaatcaaga atttacaaca aagtataaga taaggataat gctaaattgc
     1441 catacaggtg ctagatgtgt aaatggattt aattaatact attgataaag tgcaacaagc
     1501 acataaacca attgatgaga ttattaaaac tacaagagat atagttgaca taattaaaat
     1561 attggaacct atttataatt ttaaatcaaa ttaataatga ttaagattaa aataatatta
     1621 agacatcaat gttataagag tacaatataa ggagtactat atagtactcc ttacttatac
     1681 atattaggca atagggaatt tttttatagt taaattttca aatatttttt ggggaacttt
     1741 ataattgaga attaagggta tagtatgtga tgaacttact aaatttataa ttaggttata
     1801 taatgtaata atcatcaata gttttacttt agggaaaata tatgcttaag gaagtatagc
     1861 ttactaatat tattaaaatg tatatttaaa atgataaaaa aagaattaga caagtattta
     1921 gtaaattgat tatgcaaaat tctatcaagc aggaacccta tgactttagt catgggaggt
     1981 tcagtaggta acttgttgaa ttaagtttat attatttaaa taaactactt aatttagata
     2041 atataaaaag tcagaatttt aagaatagag ctatcttagc tattaggaga agtactatga
     2101 taggaatata ttttagtgga acaggaaatt caagatactg tgtagaaaaa tttttacaag
     2161 aatatgatat aacagcaaat tcttattcta ttgaagataa tgaactttta cagcacatca
     2221 ataatcatga gaatattatt tttagttatc cggtgcaata tagtaatgtg ccaaaaatat
     2281 tgaaagattt tataataaat cattcaactt tgtggaatgg taaacgtgtt tttattattg
     2341 caactatggc tctatttagt ggagatggct ctggtgtgtt atctagaata ttaaaaaaat
     2401 acggtgctat aactgttggt ggattacatg tgaagatgcc agatagtata ggagatgaaa
     2461 aggtattaaa acattcttta gagtataata gaaagcttgt aataaaagca gaaaagaaag
     2521 taactaaggc tgctaaaaaa ctgaaacatg ggtcaccacc aaaagaaggg ttgggttttt
     2581 tgagtcatat tgtagggtta tttggacaac gattgtattt tatcaataaa acaaatatgt
     2641 atacagataa actaaaaatt aatactgagg aatgtattgt atgtggtaaa tgtgttaatt
     2701 tatgtccaat gaagaatcta gtaattaaaa attgtatgat tgttgcaaat gggcaatgta
     2761 caatgtgtta tcgttgtgta aataattgtg ccaaaaaagc aattacattg ttaggtaaaa
     2821 aagttgttga acagagtaat attaaaaaat atatgtaaga aatttaaagt tttattgaat
     2881 gtaaattgat gtatatttta tgttagaaag agaggttaaa taatggaata ctacatagtt
     2941 gattcatttg caactaaact atttaaagga aatcctgctg gagtttgtgt tcttgacaga
     3001 agaatacctt tagagttaat gcagaaaata gcagaagaaa acaatttacc agaaacagca
     3061 tttgtagtta aaggtaaagg aaattatgaa ttacgatggt ttactcctaa agcagaaatt
     3121 gatttatgtg gtcatgcaac tttagcagca gcttatgtaa tatcaaattt tattgatgtt
     3181 aatgtaaaaa ggatagattt ctttactcaa agtggaaagc ttgaagttac acgaaatgga
     3241 aatctatatg aaatgatatt tccagaaata atgcctattg aaatagagtt atcaccacaa
     3301 caagctaatt tgataggctg tgtaccaagt gctgtttatt cttcaagaga tttaatctta
     3361 ttgctaaaca gtgaacaaga agttataaat tataaaccta attacgcaca attgcgtaaa
     3421 ttgacagact ggttaggtat aataatcaca gcacaaggaa gtaatacaga ttttgtatca
     3481 agatattttt gtccagaatt agattcagaa gaccctgtta ctggttcatc acattgtaat
     3541 cttattccat actggtctga aaagttggga aaacacaaaa tggtagctgc tcaactttct
     3601 aatcgtggtg gtataattca gtgtgaagtg ttgaaggata atactgtgaa aatttctggt
     3661 gaagcagtct tgtttatgca gggaacaata aagattgata tttaaataag tataataact
     3721 aatttattat gaaaaataat taattttctt agtgttaaca ataataaagt agagttaaaa
     3781 aacttgattt cacttaaatc aatatgatag atgttgatac taagattgac aagcatgatt
     3841 aaaaaagata atatttaatc tataaagata aaaacacagt ctggttggta gtccagacgc
     3901 agcaatagct gtcagtaacc tgcctccttg gttgtccgtt ctttagaaag taatttaagg
     3961 aggaactagc aatggacaaa attagtggaa aattaacagt cttatttgaa gaaccatttt
     4021 gggtaggtat atttgaaaga caagatggta aaaaatatga agcatgtagg gtagtttttg
     4081 gagcagaacc taaagaagta gaagtgtatg aatttatact tgaaagattt ttctcattag
     4141 attttggtag cattaaatta gaaaaaaatg tgaccaaaga caacattgga tacaaaagaa
     4201 tgcagagaaa agtaaaaaag gaacaagaaa aagaaactat agggactaag gctcaaaatg
     4261 ctcttaagct tcaatatgaa gaaagaaaac aggatagaaa aaacctagct aaaaatagaa
     4321 aagaagaaga aaaagaaaga ctatttaatt tgaaacaaga aaagagaaaa gctaaacata
     4381 agggtcatta attttaatta aatatctttt taataattgc tagaaaaagg tgtataaaat
     4441 ttttaatttt atacacctta atttattgaa aagaataagg ttttatattt aaattacttt
     4501 aaaatataaa aatatactaa taaaaattgt aaaaatagta taaatggtat accaaatgta
     4561 aatttaatgt gtttagtttt atgtctaaaa gaatacattc caatcataga acctatacta
     4621 cctccaatta ctgctataga cataagagta gcttctttta ttctccattc gtttctaatt
     4681 gctcttttct tatctatgta cattgaaaag aaagcaatag aattaatcac tataaaatat
     4741 attagaataa aatttttcac agtcttgcct ccataaaaat atattataca ttataaaaca
     4801 agatatttgt tttggaaagt taaatttgta attagtacat ataaaatatt tgataagaac
     4861 aaatataata ttaattggaa aatttaaaaa aattaaacaa ttagcaaata gggggtatat
     4921 ttataagaca caaagtgata aaatctattt agaaaataat gtaaaataac aggaggataa
     4981 agcaaaatga aatttatagt agaaaaagaa gtttttgata aattagaaaa tgtatgcttt
     5041 ggtgtagtag ttgcaaaagg aatagataat actaaagaaa tagaaagaat aagcaacctt
     5101 ttagatataa gtatagatag agtagaagat tacttcaaag ataaaaaagt aaaggaatca
     5161 gaagaaatta taccatatag agaggcattt agaagtctag gcatgaaccc aaataaattt
     5221 atgagttcga tagaagcaat gacaacaagg gttgctaaga ataagaaact tcctcatata
     5281 aatcctattg tagatttagg aaactctatt tctttaaaat atttacttcc aatgggtgct
     5341 catgatatgg attttagaaa tgatgatgta tatgttagat tttctaaaaa aggggataaa
     5401 tttgtaccat ttggagaaac tgatgttgaa ttaatggaag aaggagaatt aatttattca
     5461 gttggagaca tggtaaaaac tagaagatgg atatggagac aaggagaaga aggaaaaatt
     5521 actaatagct caaaaaatat attttttcct atagatggtt ttactgatgc taatttagat
     5581 aaggtaatga gtgcaagaga agaattagca aaattgttaa aggaaatatt taattgtgaa
     5641 attaaagtag gttttgtaga taaagataat cctgaaatgg aaatataata agttgttgaa
     5701 tacttgttaa gatattatat ttacttaatg atgttaaaaa ggtattaaaa aagaaaggtc
     5761 gtagtaaatt tgaaaaaaac tataataaag aatgtaaatc caatgattga aaataagttt
     5821 ataggacttt ttgaaataga gtataaaaac aaattagatg aagataaggt ttggatggta
     5881 gcatcaagaa aaagtagtga gcaattaaaa aatatttatt tgaagaatga agaagatagt
     5941 gtagatgcag ttgtaatagt tgggcttcat aaaagtagta aaaaattaat tttaataaga
     6001 caatttagag tacctattaa tggatatata tacgagttac cagcaggtct tgttgatgaa
     6061 ggagagtcta tagatatttc cgttgaaaga gaacttagag aagaaacagg acttactctt
     6121 ttagaaatta aaaaaaataa aagtattgat aaagtttatt tatctcctgg aatgagtgat
     6181 gaatctgttg cctttgttta ttgtatatgt gatggtgata taacagatga atttttagaa
     6241 ccagatgaag agattgaagc gcttttaatt tcgcaagaag atgcaaaaga gattttacaa
     6301 agtaatcata agatagacac aaaagcattt ttgattttac aaatgtttgt gagtttagga
     6361 gctaaattat ttgaatagat atatagaaaa gaggttatat agaagagtaa aataataaag
     6421 tttattttac ttgtgtaaat tataatctct ttttttatac aataaagtat aattttgtgt
     6481 atttttaaat aagtgatttg actcttaaat tttcataata tgttataaac caatttaaga
     6541 tactacaata attaaaattc caaaattatt taacatattt gcaattaaaa tgagaaaagg
     6601 taaaggctga atgattgaaa aatttgatgt aaaatagtag cttagtttaa tactatagtt
     6661 tgtagggtta aaaattagaa attgtaaggg agataaattc tatgaaaaaa ttagttatta
     6721 ttttaattag tatcattagt atggttagct tgacagcttg taaaaaaagc aatgaaagta
     6781 aatataataa tttaaacaac aaagaatccg gacaaacaac cattgaaatg gacttagata
     6841 aaaattatga tacttcagac ccatttgtta atgcacgttt gttttgtgtg tctaatgata
     6901 tagatgtttt agacacagag atttctttta aaatggatgg agatagtggt attgtggaaa
     6961 taaaggacaa taaaacagat gaaactttat ggagtaatac atggcatgga agcgttgata
     7021 atgatacatt cacaatttct ctttctaata tacaaaaaga gaaagagtat gctatatgct
     7081 ttactggaat taaaataaat catgcagttg taaaggttac ttttgaaagc aatttagtta
     7141 aggagaaaga aaaaccctca aaataaattc cagcttttct ataacttgat attcagtaca
     7201 taaaagcagt tgatatttat gattgactgc tttttcttat caaaaaggat aggttgtgca
     7261 tttttacttg aaatagttta tgcacatcaa aagttggtga gcatgatatt ggaaatggta
     7321 agcatgaaat cttgataata caaggaaata aatttaagtt ttcaattaag tatatttcca
     7381 taatttagtt aacaataagc taattattat ttatattttg atatataaat aataattgta
     7441 gcaaatttaa aaaaatcata tattttttga actaaataaa aatttatttt tattatttta
     7501 aattagagga gattcttgaa gatatattat ttttaaaaat aagtatagtt taaaaattca
     7561 aaaaaagatg gcaaaatatg tcccaaggga cattgagtaa aataaatgat ttcagcttat
     7621 ttacactaaa aagtacatac taaatgaggg gtaaaataaa gaaaaatgat atactaattg
     7681 tcttttgata ataaaaaaac atggaatttg attatttaat caagaaaaag tattgaacaa
     7741 atgatttatt tagtgtacaa taatcatgta aaaacatagt aatggaggga gacatttcat
     7801 aatgaaaatt aaggtaggaa tgaacggatt tggaagaata ggaagagctg tattaagaat
     7861 agctcaagaa gaattaggag ataatataga aattgtagcc ataaatgcaa gagcaacaac
     7921 agaatctttg gcacatttat ttacatatga ttcatgctat ggaactttta gaggtgaagt
     7981 agaggcaaag gatgaagaca ctctagtagt aaactctaaa gagataaaaa tattaagata
     8041 taatgaccca gaagaattac cttggaaaga attgggggtt gatatagtaa tagaatcaac
     8101 aggattattt acacaaagag aaaaggcaga aaaacatatt aaagcaggag caaaaaaagt
     8161 aattataaca gctccaggaa aaaatgaaga tataactata gttatcggag ttaatgagaa
     8221 agaatatgat aatgaaaagc acaatataat ttcaaatgca tcttgtacta caaattgtct
     8281 agctcctttt gctaaagtat tggatgaaaa atttggtata gtaaaagggt taatgactac
     8341 agtacattca tatactaacg atcaaagaat attagataaa agccataaag atttgagaag
     8401 agcaagagca gcggctgaat ctataatacc taccactaca ggagcagcaa aagctgtgtc
     8461 aagagtacta ccacaattag aaggaaaatt aaatggtttt tcacttagag taccaacacc
     8521 aacagtttca cttgttgatt tagtgtgcga acttaaagaa aacgttacat ctgagcaagt
     8581 taactctgtg ttaaaagatg ctgctgaagg agaattaaaa ggtgttttag gatattgtga
     8641 caagccactt gtttctatag attataaagg agattcaaga tcatctatta tagacgctct
     8701 atccacaatg gttatagaag acaatatggt taaggtagtt tcttggtatg ataatgaatg
     8761 gggatattct tcaagaactg ttgacttagt aaaatatgta gctgaaaaat taaaataata
     8821 tatatttctt aaagcaaatt ttaaaaaaat tttttaaaaa aattatataa aactaaaaaa
     8881 aagattatga aaaatattga aggaaaaatt ttttcataat cttttttctt tcatgcaaaa
     8941 agatacaagt tacaacttat atttgcatat taggtcgtta gagaagtata tatatattta
     9001 gtaaggaatc atacttttta taaaatgaaa gataagatgt cgaaaacttg cataaatctg
     9061 aaaaattttt attattaaaa tactgatttt cattttaaaa atgataaaaa aatgacaaat
     9121 aaagttgcaa atagtaatac ttataagtat aattaaaaaa gagaaaaggt tttgttaaac
     9181 tttataacaa aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt
     9241 taaggcagta agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta
     9301 tgttggtagt attatgggag caggaatgat gttcaaagta ataatgagta cagcacatgc
     9361 tttactattg gagacagttt tcttaattat ggctatggca gttcttgcag gcgcattaag
     9421 cgcactactt tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat
     9481 gaagccttta tatggactac ctggtgctag tatagtagga gctataacaa cttatttatc
     9541 agataatcca gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca
     9601 ataccaagtt ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac
     9661 aacatttatg atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg
     9721 tgcaattata ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta
     9781 taaatacaat ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag
     9841 agaaatcaga gaaggaaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa
     9901 aatgggggta gatatgggaa tggctataat tcctggagtc ctagtagtat gtacattggt
     9961 gatgttatta acttttggac catctacaga ccctgttaca ggtcaagaag tttatacagg
    10021 agctgcatat gaaggaataa aattacttcc agttattgga gataaacttg gatttatatt
    10081 agaaccatta tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg
    10141 agctgttggt gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc
    10201 taatgatata gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca
    10261 tattggtatg atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca
    10321 tacaataggt ggtttatgtg caggagcagc tgctcacttt atatttacac tcgtaggata
    10381 aatgtaatca gctaagataa aaaataatat acaaaaaaat ggttgttgat atgtaataag
    10441 aatttacata tcaacaacca tttttttctg actaaagata atttataata cataaacatt
    10501 aagtggatat tagagttaag ttatatttat gtaaaaagta acaaaaatct gtagaaattt
    10561 atgacaaaat ttagtacact aactatatgt tcttatttaa aatttaaggt tagaattagg
    10621 aattacctag tagagtaagc tttgattaat aaaagaatca taaaataaaa atattagtca
    10681 taaagattat aaataactta cttaaaataa agccatagaa cttacaaaaa ttaaaaaagc
    10741 atataaatat ggaggggtac aatggatgaa ttatttcata taggaattga tgtaggttct
    10801 acaacggtga aggttgtggt acttaataat agtaataata tagttcataa agaatataga
    10861 agacattatt ctgatgtaaa aaaatcagtt aaagaagtac taaatggtat atatgaaaaa
    10921 ttgggagata taaatactac cattataata actggttcag gtggaattgg aatatcaaaa
    10981 aaattaggtg taaaatttgt tcaggaagtt atatctagta caaaatcaat agagtatttt
    11041 catcctgaaa ctgatgttgt catagaactt gggggtgaag atgcaaagat aacatatcta
    11101 agtggaggaa tagaccaaag aatgaatgga atatgtgcag gtggcactgg agcattcata
    11161 gaccagatgg catcactttt aaagacagat gccagtggat taaatgaact tgctaaaggg
    11221 tataatgtta tatatcccat agcttcaaga tgtggtgttt ttgctaaaac agatattcaa
    11281 cctcttataa atgatggagc caatcagact gatatagcaa tgagtatttt caatgcagtt
    11341 gtcgtacaaa ctgtgagtgt cctttcttgt ggaagaaaaa tagaaggaaa tgttgcgttt
    11401 ttgggtggtc cattgtactt tttatctgaa cttagagaag catttaaaag agtattggat
    11461 ttaaaagatg aaaacataat cttcccagaa aatgcacaac tttatattgc tattggagca
    11521 ggattattat cagtagatga agataatata caactaaaat ctctaataga aaaattggat
    11581 agtataaaga atattgaaga tggagaagta aatttattag aacctttatt taaagataaa
    11641 aatgagtatg aagaattttc aagaagacat gaaaaagaaa aaataaatta tgtggacata
    11701 aattcaataa aaagtaattg ttatttagga attgatgcag gctcaactac aactaaagca
    11761 gcacttattg atgaggatgg aagacttgta tattcttatt ataatagcaa tgaaggaaat
    11821 ccattaaaaa caaccattaa agttataaat gaagtatatg atatattacc aaaaaatatt
    11881 aaaatattga gttcaactgt aactggatat ggagaaggtc ttattaaaaa agctcttaaa
    11941 atcgataacg gagagataga gactatagca cattacaaag ctgctaaatt tttcaataaa
    12001 gatgtagatt ttatacttga tattggtggt caagatatga agtgcctaaa aataaaagat
    12061 ggagtaattg ataatataat attaaatgag gcttgttcat caggttgtgg ctcattttta
    12121 gagacatttg caagttcttt atctatgaca atagaagaat ttgcacacga aggtatttat
    12181 tctcaaaatc ctgtagattt aggttcaaga tgtactgtat ttatgaattc tagagtaaaa
    12241 caagctcaaa aagaaggtgc aagtgttgga gatatatcag cagggctatc ttattcagta
    12301 ataaaaaatg ctttatttaa agtaataaag attagagatt taaatgagat tggtaataat
    12361 attgttgtac aaggtggaac cttttataat gatttggtgc ttagaagctt tgaaaaacta
    12421 attggaaaaa atgtaattag acctaatatt tcaggaatta tgggagcttt tggttcagca
    12481 cttatagcta aagaaaaata tgaaagtgga tacaaaacat ccttgttatc taaggaagag
    12541 ttgaataata tcaagctaga agcaagtgta actagatgta aaggttgttc aaatcattgt
    12601 cttcttacaa taaataaatt ttctgataat gaaattttta tttcaggtaa tagatgtgag
    12661 aagggtgaag ctatttatgg tgataaaaaa ataggattag aaaagaaaaa accaataaat
    12721 ttatttaagt acaaatacaa tagaattttt agatacaaac cattggaaaa agaagaagca
    12781 aaaaatgggg aaatagggat accaagagta ttaaatatgt atgaagatta tccattttgg
    12841 tttacttttt tcaatgctat gggctttaga gttgtacttt cagataggtc atcaaaacag
    12901 ttatatgaaa ctggtataac tagtattgca tctgaaacag tttgttatcc aggaaaatta
    12961 gttcatggac atatagaaaa tttaattgca aaaggtataa aaaatatatt ttatccaagt
    13021 gtaacaaatg aaaataaaga ggattcaaat gcagataatt attataattg tcctgttgta
    13081 atatcatatt ctgaagtaat aaaaaacaat gtagaaaata taagaaataa gaacataaat
    13141 tatataaatc catttataag tctaaatgat aaggaaaaat tgaagaaaag actatatgat
    13201 gaattatcaa gacatttttc tgatataaaa ataactaaag aagaagttaa tcatgcagtt
    13261 gataaagcta ctgaggaaca gaatttattt aaaatggaga tacaagcagc aggtgaaaga
    13321 gcacttaaag aaataaaaga aagaaatatg aaggggattg tgttatgtgg aagaccatat
    13381 catatagacc cagaaataaa tcatggaatg ccagagctta taaattcatt agatatggca
    13441 gtgcttactg aagactctat atgtcattta gccaatgtac aaagaccact tagagttgta
    13501 gaccaatggg tatatcattc aagattgtat aaagcagcta gttttgtaag agataaaaac
    13561 tatttggaac ttgttcaact taattcattt ggctgtggat tagatgcagt aactacagac
    13621 caggttcaag aaatacttaa tgaaaaatcg aagatatata ctattataaa gattgacgaa
    13681 ggcaacaatt taggagcagc taagataagg atgaggtcct taaaagcagc tatgtttgag
    13741 agagaaagca aaaatataga tgtaaaaaat ataaaggtaa acaggataca gtatgctaaa
    13801 aataatcaga taacgaacaa acatactata ttagcaccac aaatgtctcc aatacatttc
    13861 caattccttg aaaaagctgt taacttaagt ggttataata tagaaattct aaaagatact
    13921 gatagtagtg taattgaaga agggttaaga tatgtaaata atgatgcatg ttatccagct
    13981 ataatagttg taggtcagct aataacagca ttaaaatctg gcaaatatga tttaaataac
    14041 acttctgtta ctataactca gactggtgga ggttgtagag ctacaaatta tattggattc
    14101 ttaagaaagg ctatatatga tgctggattt aaagatgttc ctgtcattgc acttagtgta
    14161 aatggaatag aagatagtgg tataatggat aatatatcac taaaattaat caatagactc
    14221 tttatgtctg ttgtttatgg tgatttgctg atgaaagtac tatatagagt aagaccatat
    14281 gaaaaagtta ctggaagtgc aaatgctcta tatgaaaaat gggttgatat atgcaaatct
    14341 tctcttgtta aagcaaaaat ttctgtattt actagaaata taaaagatat agtaaaagaa
    14401 tttgataatt tagagatact agatatcaaa aaaccaaaag taggtttggt tggagaaata
    14461 ttggtgaaat tccatccaat tgcaaataat aatttagtag atatattaga gcgagaaggt
    14521 gctgaagcag tcgtgccaga ccttacaaat ttcttcttaa gttgtgcctt taatactata
    14581 tacaaacaca ctcatttaga aggaagtaga aagagtagaa tgataggaga ggcatttata
    14641 tacataactg gaatatatca aagagtatat aaaaaagcac ttgataagag tgagagattt
    14701 tatgctccag ccaatataaa aaatgttgct aaaagtacag aaccagtagt gtcattaggt
    14761 aatcaaactg gagaaggttg gcttttaaca ggtgaaatgg ttgaattatt aaatgaaggt
    14821 gtagaaaaca ttatttgtat gcaacctttt ggatgtttac ctaatcacat aattggtaag
    14881 ggttctataa aagaattaaa gaggttgtat aaaaatgcaa atataatacc tattgattat
    14941 gacccttctg caagtgaggt aaatcaactt aacaggataa aacttatgct ttctaaggct
    15001 tttaaaaata tataagattt atattttgat atgttaaaat ttaatgaatt taaaaatata
    15061 ctctatatta agtatctata aaaaagagtt atcctatttt ttagggtaac tctttattag
    15121 ttaaaaaatt atatgtatac tgatattaaa ggggggaagt aatatgagtt tagtatatga
    15181 tgtttataaa tcaaaattag gaaatttgta catattaagt gaagatggtt ttatagttag
    15241 tatatacata ggagatgaaa aatttaataa tttaaaaaat gaatgtatta aaaaaggaat
    15301 tgatataaag agaagtaagg aagatttaaa ggaagcaata agtcagatag atgattactt
    15361 taatggaaaa agaaaagtct ttaatttaaa tattcgtatt gaagggactg aatttcaaaa
    15421 atcagtttat agggaaatgt taaaaatacc ttatggagaa acattgtgtt attcagatat
    15481 agcaagaaat ataaacaatc caaaatcagt aagagcaata ggacaagcaa gtaaaagtaa
    15541 taaaattcca ataataatac cttgtcatag agtagttggc aaaaaaaata ttggtggata
    15601 tatggggaat cattctgatt tgaaagagat actattaaat ttagaaagac agagttaact
    15661 tagaaagaaa tgtttttatt gttaatattt gatataataa tagtaaaaag aagtattttt
    15721 tacaaaaaca ataaagaatt tattttaatt tacaaaaatg agggggaatt ttaatgtgtg
    15781 ataaagttat agcatcaaaa tatcatgaga agggatttaa ttgtgcagaa tcagttataa
    15841 aagcatataa tgaggaattt aatacagata taccagtttg tttaggaagt ggactgggtt
    15901 cagggtgtgg ggttgctagt ttatgtggtg cagtaaatgc atcaaatata ataattggat
    15961 acgtaaaagg cagaaaccac gaagatgaaa gtactaaagc taaagtatat gctaaagatt
    16021 taactacaac agtaagaaaa gagtatggtt cagagttatg tatagaccta aaaaaagatt
    16081 tagttgcttg tagagaaata atggatttcg catatgatag tctaaaggaa actttaaaaa
    16141 aagaattata agaaaataag ctatcaagta taaattattt gatagcttat ttttatagtc
    16201 gatactaaat aaatcaaata tattttagaa ttattatttg taaataaaca gcaaataatt
    16261 ttaggatttt tatttaatat agtttttatt tatatttatt tttgaatatg ttatactttg
    16321 tttaaataaa tatttgtaat gagggagatg agaagattga agaatctcaa tttacttatt
    16381 aataaattat attcaaaaaa tcataaagaa gcatataaaa cttttttatt cttagaaaat
    16441 gaaagtttaa aatcaaatat tacatattgt ttttttgact cattcctaga aatgattgat
    16501 aacgaaaact catatattcg aacaagggga ttgttattaa tctctgcaaa tgctcagtgg
    16561 gatattgata ataaaattga aataaatata gatagtatac taagtcatat cgtagataaa
    16621 aaaccttctg tatcaagaat gtttattaaa tccataccta atattacaaa gtataaagaa
    16681 aatcttatta atcgcataaa aatggaatta agtaatgcag atataagtat ttataataat
    16741 aatatgaaac cattagtaga aaaggatata aacgat
//
LOCUS       DAELJO010000072        16735 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELJO010000072 DAELJO010000000
VERSION     DAELJO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.072"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            121..498
                     /locus_tag="KPB88_001990"
     CDS             121..498
                     /locus_tag="KPB88_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0054269.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLIDKGYLARDIIKFQSYYKIIIDNEEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKNVSKEKLDSLEEYYKNLKE"
     gene            603..986
                     /locus_tag="KPB88_001991"
     CDS             603..986
                     /locus_tag="KPB88_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0054270.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            1171..1755
                     /locus_tag="KPB88_001992"
                     /pseudo
     CDS             1171..1755
                     /locus_tag="KPB88_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            1781..2194
                     /locus_tag="KPB88_001993"
     CDS             1781..2194
                     /locus_tag="KPB88_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054271.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECKYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            2198..2941
                     /locus_tag="KPB88_001994"
     CDS             2198..2941
                     /locus_tag="KPB88_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG0054272.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            2941..3411
                     /locus_tag="KPB88_001995"
     CDS             2941..3411
                     /locus_tag="KPB88_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054273.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            3428..4186
                     /locus_tag="KPB88_001996"
     CDS             3428..4186
                     /locus_tag="KPB88_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054274.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            4170..5012
                     /locus_tag="KPB88_001997"
     CDS             4170..5012
                     /locus_tag="KPB88_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0054275.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(5172..6077)
                     /locus_tag="KPB88_001998"
     CDS             complement(5172..6077)
                     /locus_tag="KPB88_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0054276.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            6439..7173
                     /locus_tag="KPB88_001999"
     CDS             6439..7173
                     /locus_tag="KPB88_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054277.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            7384..8565
                     /locus_tag="KPB88_002000"
     CDS             7384..8565
                     /locus_tag="KPB88_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG0054278.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDTITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            8549..9121
                     /gene="mobB"
                     /locus_tag="KPB88_002001"
     CDS             8549..9121
                     /gene="mobB"
                     /locus_tag="KPB88_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG0054279.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILPNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            9240..9515
                     /locus_tag="KPB88_002002"
     CDS             9240..9515
                     /locus_tag="KPB88_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054280.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            9770..11470
                     /locus_tag="KPB88_002003"
     CDS             9770..11470
                     /locus_tag="KPB88_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054281.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            11833..13020
                     /locus_tag="KPB88_002004"
     CDS             11833..13020
                     /locus_tag="KPB88_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054282.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            13080..14243
                     /locus_tag="KPB88_002005"
     CDS             13080..14243
                     /locus_tag="KPB88_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0054283.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            14394..15197
                     /gene="zupT"
                     /locus_tag="KPB88_002006"
     CDS             14394..15197
                     /gene="zupT"
                     /locus_tag="KPB88_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG0054284.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            15292..15852
                     /locus_tag="KPB88_002007"
     CDS             15292..15852
                     /locus_tag="KPB88_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG0054285.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            16103..>16735
                     /gene="rgbR"
                     /locus_tag="KPB88_002008"
     CDS             16103..>16735
                     /gene="rgbR"
                     /locus_tag="KPB88_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG0054286.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESG"
BASE COUNT         6582 a         1713 c         2978 g         5462 t
ORIGIN      
        1 tttacactta aaaaaacaga ctaaatatgt agaatatttg gtaaaactta cctctagttt
       61 ttgtcttaaa aagctcctat tattaattta agttagacga atttaaggaa gtgacataaa
      121 ttgaaagaag aaatattaat agaaaaatta ttaagagcag aattaatagt aatggagtat
      181 ttatggaaaa aggacactct aatgcctaaa aaagaaattg taaaagaaat aaaacagatt
      241 tatggatggc ataaaagtac aataaaaata ctactaaaaa ggctaattga caaaggttat
      301 ttggctaggg atattataaa atttcaatct tattataaaa taataataga taatgaagaa
      361 tattatgctt ttaagaaaaa ggtactaaaa tctagtaaaa gtagaaaaat aatgcgttca
      421 cttaccacta cgcataaaaa tgtaagtaaa gagaagttag actcattaga agagtactat
      481 aaaaacttaa aagaatgata tggtaaaaat tggatggaat tatttacctt aattctcttg
      541 tttgtaaaac tttattgata tagaatttaa catatacata aatcggtaaa ggggtgtaga
      601 ctatgaaaat tagtaaatta ccagaagcgg aattaaaagt tatgagatat atttgggaag
      661 ctaacaaggt attaacttct agggaattag ttgctgcaat ggaagaaaaa tatgaatgga
      721 atgaatcaac gacatttaca gttttaaaaa ggttagagcg aagagaattt ttaagcacag
      781 agaaaatagg caaacttaca cattacaata tactagtaaa agagaaaaaa tatctaaggt
      841 ttgaaacgaa agaatttttg aagaacatac ataagaattc aatttcaagt ttaatatcag
      901 cgttacatag taaagatgat gaagtggatg aagataaatt aattgaactc gaagaacatt
      961 ttaaaaattt aaaagagaaa gaataaataa agattatttt aggtggcaaa ttatttttgc
     1021 cacctttttt gccaccatga cagtttaata aaataaaata caaataaaga aatataaata
     1081 aaatagaata aagaaattgt tttagatagg ttgtaaataa atgtactcat aaaaacataa
     1141 ataaatataa ataaacataa ataaaatata agaatttctg tgagaaatgc acttaatgaa
     1201 cttgtaaaag aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca
     1261 gagagaatag aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat
     1321 gcaaaaccaa gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct
     1381 gaagctttaa gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata
     1441 gatgaagtac ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta
     1501 aaagaagaat tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta
     1561 cctaaacgag tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgaccat
     1621 cttaaatgtg caagtaatat gccactattg aaagtaagac aatcggtgta cacagaaaat
     1681 aatgatgtct tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta
     1741 tcagcagaaa tataatctat aatttagtag gaggatgagt atgaaaaaga taatattagc
     1801 atctcatgga gacttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt
     1861 agcagattgt gtcagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt
     1921 agaattagaa aaaacaatat cagaagataa agaatgtaaa tatataattt tatctgactt
     1981 atatggagca agtgtatgta cagcaatgtt aagattaact aatctacata atgttaggtt
     2041 attttctgga atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt
     2101 aacagttgaa gatatagacc aattagtgga agaatctaga agaggaatac aaagcgtgac
     2161 attacaaatt aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc
     2221 agtatttaaa agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga
     2281 agatatcttt gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg
     2341 aataatgtta gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt
     2401 atcaaaagct aatttatcaa taccatatgg agtaaactgt ttaaatgttg atactatggg
     2461 atttgaactg gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca
     2521 tgttaaacct agagatgaag ctactttaga agagtttttt aaattacaac gttcaaaatg
     2581 cccagcgtat ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga
     2641 tgttgaagaa gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa
     2701 tgctacaggt caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga
     2761 cttcccattg gttatagctg ctggagttac attggaaaat gcaaaaaaac agcttaaatt
     2821 aggagatatg gcaataatag gaagttactt taaagataat tacaaagatt ttggggatgt
     2881 tagtgtagaa cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata
     2941 atgattaaat tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg
     3001 acaaagtttt taagtacaga ctgtatattg atagcaagtg atgacttatt aaaagatgaa
     3061 ttgagaatgg caggacttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc
     3121 atagcagatt ctataaaagc acttaactca ggtgttactg ataaatacaa cttattgata
     3181 ctttgtgaat ctgtagaaga tgtttataga cttgctaaag aggtaaaagc tattaaatct
     3241 ataaaccttg gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat
     3301 gtatcaaaag atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt
     3361 gtacaattag ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg
     3421 gatgaaaatg gaatttacac aagttatttt gataactcta attgcattct ttgcatatat
     3481 gcatagtttt gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg
     3541 tttagcacta ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt
     3601 tatgggtgca tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat
     3661 tgccacagcg tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt
     3721 tcctatagca actctagtat tgcttataga taacttctta atgacagtag tacttacatg
     3781 gggagctcat attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt
     3841 gcaattatta tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt
     3901 ttcattaggt gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca
     3961 tggaatggat gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctagaat
     4021 gatgcttaat aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct
     4081 aaacataaca gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa
     4141 ctttgtagga gagaaggagg taatagtaga tgacaacgag ttctaagaaa ttagagacaa
     4201 tttcaccaga tagtaaaata acgaggaaag acttttggaa atgttttaga aggtcattga
     4261 cattagattc atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg
     4321 caccaataat ccgtagatta tataaagatg ataaagaaaa gaaatctaag gctttaaaaa
     4381 gacatttaga atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg
     4441 gagctatgga agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg
     4501 ttaaatcaag tttaatgggt ccagtttctg ggataggaga ttcatttttc tggggaacat
     4561 taaagcttat agcagctggg gttggtatag ctttagcttc tcagggtaac ataatgggac
     4621 cgattttatt ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag
     4681 ataaaggatt taagtatgga actcaattct ttaaagatgt gagtggttca agtatagttt
     4741 caaaagttat ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat
     4801 ctaatgtaat gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc
     4861 aaacttactt agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt
     4921 attggttatt aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt
     4981 gtatagtatt ggctgctatt ggagtagtat aaataaaaaa atcccaagtt catttgaacg
     5041 agggattttt tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat
     5101 ttatgtgata gttataatta gcttattaat tagttataaa ctcaaaatta agcactactt
     5161 tattaaagtt attatattat aatttcgaaa tactcacata atctgtttag aaatttatct
     5221 tctaatgggg aaaaagtttt ctttttagag taaatgaaga aaaactttct agtaaaatca
     5281 acatccttta ttttataaca ttttatttta cctgcattga gataatcgat agctgaaatg
     5341 tacgatataa aggatactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat
     5401 tctacatgag cgattacatt taatttactt actggaaagt tatttttact aagtgtgttt
     5461 agtattaagt ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat
     5521 tctcctatat cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga
     5581 acaagttcat catctaataa gtctaggtac tctatttgag gattattgat tttagaacca
     5641 actaatccaa agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa
     5701 tggcttatgg aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt
     5761 aaaatataag tttcaggtat ggaactacat gctatatcta tgataccttc tatttttcct
     5821 gaatattctt ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt
     5881 tcataaagaa tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga
     5941 acagtttcta gctctctctc taaattctgt atgtgtgcgc ttacagtagg ttgagttaaa
     6001 aaaagttctc ttgcagcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca
     6061 agttgtttaa aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt
     6121 tgataaatta atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt
     6181 ttttgattgt ttaggaataa tttggtataa tgttgtaagg tacttacaat aaaaatatcg
     6241 taaattgaat tttcaaaatt caactttaaa gtgtttttta ttacagaaaa attacaatta
     6301 gcttcttaat cttattaaat tcaaataaat attatataat tattttataa tataataaat
     6361 ataattattg aaaaaagaga taactgtaaa ttgagatata gataaatact ataaaattta
     6421 agaaaggagg attgatttat gaagttaaaa aggtcattag tttgtgtaac tattttaggt
     6481 attatacttg ttggatgtca taaagaaaat actaaagaga aaaatcaagt agcaagtaaa
     6541 gcaacacagc aaaaaactat gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa
     6601 gattataagt acattataaa gaatatgggt ataccttata atacatttta ttatataaaa
     6661 cctaaagttt taaaagaatc aaatacaatg caagatataa atacatcaag ttatatgaca
     6721 ttagtttatc caaaatatac tggaaatgat gaactagatg gaagtgcttt atatgttgat
     6781 attaacgaaa ataaagttgt caatgttgaa accaattctt tctcatcaca aggtatatca
     6841 gtcattgatg ctgaatctag tatagtgatt gaaaaaagtg accatgaaaa atcggctgta
     6901 tcactagaaa attttagaca tattgacctg ggagagtatg ttggtgttga agattccaga
     6961 ataaatgaaa ttgtaggaga tgcgaattat gatttaacag catataatca tgaaggcagt
     7021 aaagtagtta agagttatag attgaaagaa gataacaaaa tattaaaaaa agaggtactt
     7081 acaataagta ttgtggataa caaaataaaa tctataaaaa ctattgaaag tgataaaatt
     7141 gtaaaaataa taaaaggaac tttattagaa taaaatattc taaagcatat gatgctgttt
     7201 caagatagaa ggaaatttct agctgggaac agttttttta ttagatttat aaaatttgaa
     7261 ctgcacttat agataactat tagttataat taaatataag ataaaagtat ttccaacttt
     7321 tataatctat aatatattac atagaaatat cttaaataaa aaatatattg gggagtattt
     7381 aaaatgatag aattagaaga tgccataaaa attattgaaa aaaatgtttt accaataaac
     7441 agggtcaaga tagtaaatct tatagatgca gtaaataagg tagttgcaga agatatatat
     7501 tcaacaattg atagtcctcc atttgatagg tcaccctatg atggttatgc atataatgca
     7561 aattctgaaa atagaaattt aaaggttata gggactttgt atgcaggaga tgtatttaat
     7621 agggtattaa ataaaaatga agcagtgaag ataatgactg gtggaaagat accacagggt
     7681 gcaaattgtg taataaaaaa agaagatgta acagttatag gtgatacgat aactttaaat
     7741 ataaaattaa aggaatacga taattacata tttaagggtg aggatataaa aaaggggcaa
     7801 ttgattatta ataaaaatca gaatatagga tatgatggta ttggagtatt agcaagtctt
     7861 ggaatatcca aagtaactgt ttatgatgat ttaaaagttg gaattttgaa tacaggtaca
     7921 gaactacaag atataaatga gactttagag aatggtaaaa tatacgatag taatagatat
     7981 accttatatt caagattatt aaaacttgga atacaagcag atacaatatg caattgttct
     8041 gatgatatat tggagttaga aaaaaaggta aagaatatgt tacagagtgt agatttttta
     8101 attaccacag gtggtgtttc tgttggagat aaagatttaa ttccagatgt attcaaaaaa
     8161 ataggggcaa cagaattatt ttggaagata aacattaaac ctggtggagc ttgttatgta
     8221 ggtacgttgg gagaaaagct attatttggt ctttctggta atccacatgc agcaatagtg
     8281 gtatttgata atgtgatagt tcctgttatt gagtatttaa catataagaa gggaacacat
     8341 catataaaag ctatatttaa aggagaattt aataaacttt caaataaaga tagatttgta
     8401 agtggaaaac tatatacaag tgaaggtaaa ttgtatgtag agctaaatga taagaaagat
     8461 gcaaaagcta gattatctgc aactctaaga agtaattgta tgattaagat taaaaagaat
     8521 gagacaataa aagaaaatca attagtggat gttgttatta gatagtttta tgaaaggaca
     8581 caattttatg tataatatat tgtcagttgt ctcatattca ggagtaggta aaactacgtt
     8641 gattgaaggc ataataaaag aattaaataa aaaagggtat cgtgttggag ttattaaaca
     8701 tacctgtcat gattttgata tggatgagga aggaaaagat acttataaac atagaaaatc
     8761 tggggcgaga aaagtctgta taatatcaga taaacgagta gcatatattg aagaactaaa
     8821 agagaagaat ccattatata aaatgattca attatatgaa gatatggact tgattatcat
     8881 agaaggatat aaaaattata gatttaaaag attggaagta actagaaaag gtaagtatga
     8941 ggatatttta cctaataagt ctgatttgat aggtattgtc agtgatatag aatataaatt
     9001 gaatattgaa cagtttaatt taaatgatta taaaaatata tcaaagtaca tagagtcttg
     9061 tataaaaaat gaaattttaa gtataagcaa tgttgaaata aaaaacatat taaaagaata
     9121 attttaagat gttcttctta tataaaatga tataattatt tatataaata aagatataag
     9181 aaatttatca attttatttt gagttataaa atattaaaga aattgtagga ggacaatata
     9241 tgaaaaaaat attagtagcc tgtggagcag gaatcgcaac atcaacaata gtttgtgaca
     9301 gagtagaaag attggttaaa gaaaataata tacaagcaga agttgagcaa tgtaaaattg
     9361 ctgaatgttc aacaaaacaa gaaggtgctg atttaatcgt atctactact atactaccaa
     9421 caacttatga tatacctaca ataaaggcaa caggatatat aacaggtata aatacaacag
     9481 cattagataa aaagatatta aatgcattga agtagttttg tatttataaa aaaaatttag
     9541 tattaaatat agagttgtat ctttataaaa tttaaaatat agagatatag ctctttttat
     9601 gttgtttcaa gtatacaact atatgatata aatcttcaat tttatgaaaa agattgttat
     9661 tactttgtaa gtttataaaa gatttaaagt tattgttatg ttatactaat ttatgggtat
     9721 tttttagaag tactaataga agtactaata aaggagggat ttaatactta tgataaaaca
     9781 atttgtcaaa tattataagc catataagaa aatttttaca ctagacctta tagcagcttt
     9841 tctattttcc ttatgtgatt tagtttatcc tatgataact agaaatataa tggatgacgt
     9901 tgttccaaat aaaaacttaa gaatgttagt agtttttgca gtagcactta tacttatatt
     9961 tatagcaaaa gcagggctta actattttat gcagtattgg ggacatgtta ttggtgtaga
    10021 tatgcaagct gatatgagaa atgaggtatt tacacactta caaagacttc caaatacgta
    10081 ttttgataat aacaaatctg gagtaactat gtctagaata gtaaatgatt taatggatat
    10141 aacagaactt gcacatcatg ggcctgaaga tttattcatt tctatagtaa tgttggttgg
    10201 ttcattcttt atacttatag atataaatat tcctcttacg cttataatat ttgctatatt
    10261 accgtttata atttggtttg caatagctaa aaaagataag atgaatattg cttttatgaa
    10321 gagtagagtt actataggtg atgtaaatgc tactttagaa aatagtatag caggtatgaa
    10381 ggtaactaag tctttttgta ctgagaaaga agagttaaat aagtttgtaa gaagcaataa
    10441 attatttaga agagcaagac aagattctta taaagtaatg gcagaatatt actctggtat
    10501 gaatttatac atggatatct tagagtgggt agttgtaata gctggtggtt attttactta
    10561 tataggaaag attactttgg gtgattttgc tgcttatata ctatatgtaa aaatgtttat
    10621 acaacctatg aaaaaattaa taaactttac agagcaatat caaaatggta tgacaggttt
    10681 taaaagattc atagagataa tggaacaaga ccatcaaaaa gaagctaaaa atcctattga
    10741 acttgaaaat gtaaaaggtg atattgagat agaaaatata tctttcacat atgaagataa
    10801 gactcaagtt ttggataatt taagtttatc tataaaagct ggtaagacta tagcattagt
    10861 tggaccttct ggaggaggaa agactacatt atgtaatcta ttgccaagat tttatgaatt
    10921 tgataagggt gatataaaaa ttgatggaaa gagtataaaa gatgttagtt taaaatcatt
    10981 gagaaaaaat ataggtatag ttcaacaaga tgtattttta tttacaggaa ctataagaga
    11041 taatattctt tgtgggaacc ctaatgctac agatgaagaa atgatagctg ctgctaaaaa
    11101 agctagaata catgactttg tagagacttt gccagatgga tatgatactt atataggaga
    11161 aagaggcgta aaattatcag gtgggcaaaa acaaagaatt tcaatttcaa gaatcttttt
    11221 aaagaatcct ccaataataa tattggatga ggctacatct gctttagaca atgtaacaga
    11281 aagagaaatt caagagtctt tagaggaatt aagtaaagat agaacaaatt tagttgttgc
    11341 acatagattg actactataa aaaatgctga tgaaatcata gtattgacgg ataaaggaat
    11401 tgaagaaaga ggtactcatg aagagctagt aaacaaaaat ggagtatata gtaggttaca
    11461 caataactag tatacaaatt ttacaccata gctaacatac acattaaact tacaaacata
    11521 ggatatatta agattcataa agacaattca ttttatattt ataagatgtt ttatgaataa
    11581 aatgtatcct atgttttatt ttttgcaaat tacatttaat tctctttgta agaatagagt
    11641 taaatagcca atggtcggga taacgttttt acaaaaagaa taatttagaa aactaacttt
    11701 aatttatgga atttatcgaa ttttatgaaa aaaatcgaat tttgacttga aatgtttttc
    11761 aaaagtagta aaataatgga taactataca tacattttat aaaaatatat ttattatgag
    11821 gggggatttt gtatggaggc aataacagct ttttgtctag ttttagtatc actgacgata
    11881 ggagatatag tttcagcaaa aactaaagct tttgttccgt cggtttttgt atcagcaatt
    11941 atatttatag taggattttg gacttttttc ccagaaaata ttgtggactt agccgcttta
    12001 ggaacaccat tagctcaatt aggtatgctt ttgttaataa ctcatatggg aactatgatg
    12061 agtattaagg aattagcagg tcaatggaaa acaatagtaa tagccttggc aggaatagta
    12121 ggaatatgtg taggtgcttt ggcattaggg acagtagtat ttggatggga tacagcagta
    12181 atagctacac ctcctcttac tggagggcta gttgcatcta taatgatggc agatgctgct
    12241 acagcaaaag gtcttgcaag tttatcagta cttgcaattt tgatgtatgt tgcacaagga
    12301 tttgcgggtt atcctattac agcattaatg cttaaaaaag aaggaaaaag acttttatca
    12361 gactttagaa gtggaaaagt aactgtaaat acagatgaag aaaaagttaa ggatttacct
    12421 gaacaaaaaa gcagatttaa gataatacca gacttaccag aaaaatatga tacgacatat
    12481 atgataatac ttagattagg atttgtagca tggttagcag tacaatttac aaatcttaca
    12541 aatgaagttg tatcgagata tgttatatgt ttaatatttg gtgtaatagc atcagaactt
    12601 ggaatagttg atagaaaacc acttaataaa tctggttcat ttggatggtt aatgacatca
    12661 ttgatggctt ttatatttgc agggttagca aaagctacac cagcaatgct tgttgaaatg
    12721 gcagttcctc ttgtaggaat cataatattt ggtgtaatag gtatgggaat attctctatt
    12781 atagtaggta agcttttagg tgaaagtaaa gaaatggctt tttcagtttc gcttacatcg
    12841 ctttatggtt ttccaccaaa ctatatatta actgaagagg cagtaaaggc tttggctgaa
    12901 acaccagaag aaaaagaatt tttaatggat aagatgcttc caaagatgct tgttggagga
    12961 tttactacag ttacaatagc ttctgtagta atagcgggta tctttgtaaa tctaatatag
    13021 tatttaagtg ataaaaagta ttttaaataa gaagaatgtg tactacttgg aggtatggaa
    13081 tggatattaa agaaataaca aagagttata aagactatgt aattaagctt agaagggaat
    13141 ttcatgaaaa tcctgagaaa agtatggaag aagtgagaac atcaaagaga gttaaagaag
    13201 aacttgataa aatagggata ccatatgtat cagctggagg tacaggtgta attgcaacta
    13261 taaaaggagc taatccagga aagactgtag cacttagggg tgatatggat gctctacaag
    13321 ttgttgaatg cactgatgta gaatacaaat ctaaaaatga aggacttatg catgcttgtg
    13381 gtcatgatgg tcacacatca atgcttctag gggcagcaaa agtgttaaat gatattaagg
    13441 atagtatcaa tggaactgta aaattatttt ttcagccagg agaagaagtt ggtaaaggtg
    13501 caagagctat gattcaagat ggtgctatgg aaggtgtaga tagtgtgttt ggaatacatc
    13561 tttggactga tgtagaatct gggaccatat cggtagaaga aggaccaaga atggcttctg
    13621 ctgatttctt taaaattact gtaaaaggta gaggtggtca tggttcactt cctcatcaag
    13681 gtgttgatgc agtgcttgca agttcagcaa tagttatgaa tctacaatct atggttagta
    13741 gagaagtaag tcctctggaa ccattagttg tgagtgttgg agttttaaat tctggaacta
    13801 gatttaacgt aattgcgagt gaagctgtct tagaaggtac aataaggcta tttaatccag
    13861 agcttagaaa gcaaattcct ggaattttag aacgtatagc aaaatctaca gcagaagcat
    13921 atagagcaga tgctgaatta gagtatgggt acttaacacc agcagttata aatgacaaag
    13981 aatgttcaaa gatagctaca gatgcagcaa ttaagctatt tggagaagac tgtataacac
    14041 tatttgaaaa agtaacagga gcagaagatt tagctgagtt tatgaatata gcacctggtg
    14101 cactagcatt tgtaggagct agaaatgagt caaaaggtgc ttgttatcct catcatcatg
    14161 gatgctttaa tatagatgaa gatgctctag aaattggaac tgctttatac gtgcaatatg
    14221 cagtggattt cttaaataaa taattttaat atactataaa gaaaaagcag agactaagtt
    14281 tatatctctg cttttttatt gtgtatattt tttataaata ttgactatat aaaatatata
    14341 tgatagcata aagaaaatga taatcaatat caattaaaaa gaaggtgatt tatttgaata
    14401 atgtaatatt tgcatttttg ataacattat tggctggttt atctactggt ataggaagtt
    14461 gtatagcatt ttttgctaaa aaaacaaata ctaagtttct ttctataagc ttaggatttt
    14521 ctgctggtgt tatgatttat gtttctatga tagaaatttt tcctaaagca caagatgctt
    14581 tgacaaaatc tatgggagag aaattaggaa gttggtcaac tgtaatagct ttttttatag
    14641 gtatggccat cattgctgtt attgataagc taattccaca agaagaaaat ccacatgaaa
    14701 taaagaaaat ggagaatata gaagaaaaga atatcaaaaa aaataagtct cttttgagaa
    14761 caggtatatt tacagcaatg gctatagcaa ttcacaattt tcctgaagga ttagctacat
    14821 ttatatcagc tcttgatgat gttacaatag ctattccgat agcaattgca attgcaatac
    14881 acaatatacc agaaggtata tctgtatctg taccagttta ttatgccaca ggagataaaa
    14941 agaaagcttt ttattattcc tttttatctg gtatgtctga gcctttagga gcgattattg
    15001 gatatgtact tcttagaaat tttttaaatg atataactct tggaattgta tttgcaattg
    15061 taggaggaat tatggtattc atatcactag atgaattatt gccatcagct agagagtacg
    15121 gggaacatca tttatctatt tatggattga ttgcaggaat gggtgttatg gctattagtt
    15181 tattactatt taaatagctt ttaaatatat gattgtaacc taaaattagt atcaaaatga
    15241 tataattatt taaatagatt tttaagctat ttaaaaatat cattttggag aatgagtgct
    15301 atggaaaaga aaataaaatt caaatttgac tggtatagtt cacctgataa gatagagaaa
    15361 tggttagaag atattgaagc taaaggatat aatttatata agataaataa atttggagga
    15421 atattttatt ttcataaggg aagtccaaga aaaataaaat acactgttga ttactactat
    15481 gatgtagaga ataattattt tgatgaacat aaatcaaatg gctggaatct tgtatttagg
    15541 agtattggta gttttatggg tacatctgca tggtatatat ggtcgaaaga atacaaagac
    15601 atacctccta aattatatag tgattctgat aaaaaaaaca aagtaaagtc aattttattt
    15661 atgaatatgt ttatattctt tatgttagta gtaatattta tatatttatt gaatattgaa
    15721 ataagaaatt tatatagtgg ttatggagca tctgtaactg ctattgtact acagattata
    15781 ttgttgattt tatatgcagt acaattatat aggagattaa tgtattattt aaggcttaaa
    15841 aagtttaaat aaataaaaga aggtgtccta aaattatttt tttaatattt taagacatct
    15901 tcttttgtta ataaagtact gtttttaatt atagtaattt ctgtctttaa attgaaatat
    15961 caaataatag ttgtatggga ttgagtattg ataaaaataa tttaaaaatg ttatcctaaa
    16021 gtatcagata tacaaaatta tctttaaata tattataaat attttaattg tattaactag
    16081 aaattatgta aggatgcatt ttatgattaa gatagcagtt tgtgaagatg aaaaagaaac
    16141 acaactttta atagaagatt accttgagaa tatattaaaa gatataagta tagaatatga
    16201 gatacaaaag tatatatctg gagaagagtt actggaaagt aatttaaaag atatagatat
    16261 attacttctt gatataaaaa tggaaaaatt aaatggaatg gatactgcaa gaaagattag
    16321 agaagtagac aatgaaatgg aaataatatt tgtaacttca ttaatagatt atgtgcaaga
    16381 aggttatgag gtaagagctt ataggtattt actaaagcca atagagttgg aagaactaaa
    16441 aaaacatgtg ctaacttgta tcaaagatat tgaaataaat aaggaaagtc atattacaat
    16501 aaaaaataag tctaatacat ataagattta tttaaatgaa ataaaatata tagaagttca
    16561 aaaaaaagat atgctaatac acacaataaa caagaatttt gatataaaat atagtttagg
    16621 caaaatagaa aaagaattaa atccatataa atttataaga tgccataaaa gttttatagt
    16681 aaatttaaga tatgttgaaa atataaagcc taatactgca atactggaaa gtgga
//
LOCUS       DAELJO010000073        16718 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELJO010000073 DAELJO010000000
VERSION     DAELJO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.073"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..801
                     /locus_tag="KPB88_002009"
     CDS             <1..801
                     /locus_tag="KPB88_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0054287.1"
                     /translation="IDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFKL
                     AIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKDG
                     LTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDII
                     RSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIYS
                     KIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
     gene            809..2809
                     /locus_tag="KPB88_002010"
     CDS             809..2809
                     /locus_tag="KPB88_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054288.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3071..4330
                     /locus_tag="KPB88_002011"
     CDS             3071..4330
                     /locus_tag="KPB88_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0054289.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4391..6178
                     /gene="iorA"
                     /locus_tag="KPB88_002012"
     CDS             4391..6178
                     /gene="iorA"
                     /locus_tag="KPB88_002012"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG0054290.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6180..6755
                     /locus_tag="KPB88_002013"
     CDS             6180..6755
                     /locus_tag="KPB88_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG0054291.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            6808..7878
                     /gene="buk"
                     /locus_tag="KPB88_002014"
     CDS             6808..7878
                     /gene="buk"
                     /locus_tag="KPB88_002014"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0054292.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8096..8797
                     /locus_tag="KPB88_002015"
     CDS             8096..8797
                     /locus_tag="KPB88_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054293.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            8857..9363
                     /locus_tag="KPB88_002016"
     CDS             8857..9363
                     /locus_tag="KPB88_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG0054294.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9538..10560
                     /locus_tag="KPB88_002017"
     CDS             9538..10560
                     /locus_tag="KPB88_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0054295.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(10707..11015)
                     /locus_tag="KPB88_002018"
     CDS             complement(10707..11015)
                     /locus_tag="KPB88_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0054296.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11075..11503)
                     /locus_tag="KPB88_002019"
     CDS             complement(11075..11503)
                     /locus_tag="KPB88_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054297.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(11726..13183)
                     /locus_tag="KPB88_002020"
     CDS             complement(11726..13183)
                     /locus_tag="KPB88_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0054298.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13601..14515
                     /gene="nadA"
                     /locus_tag="KPB88_002021"
     CDS             13601..14515
                     /gene="nadA"
                     /locus_tag="KPB88_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG0054299.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            14528..15829
                     /locus_tag="KPB88_002022"
     CDS             14528..15829
                     /locus_tag="KPB88_002022"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG0054300.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            15810..16646
                     /gene="nadC"
                     /locus_tag="KPB88_002023"
     CDS             15810..16646
                     /gene="nadC"
                     /locus_tag="KPB88_002023"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG0054301.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         6496 a         1792 c         3066 g         5364 t
ORIGIN      
        1 atagatgaag gtataagttg ttatataaaa ggtttaaatt tagttaaaaa gagttcattg
       61 aatcttagaa aaatatctat attttatggg ctaatagcaa atggatatga gctaaaaaaa
      121 gattttaaac tggcaataga atattataaa agtatggatt ggtattctga taggtgggaa
      181 aaaaataatt cctataaaac atctcaagca attcacaaac aatatgaact cagacaaaaa
      241 caagagagtt taagattact agtggatgat aataagaaat tagaaaatga tattttaaaa
      301 gatggactta ctaaactata taatagaaga tatttggaac agcagttgga tttatttaat
      361 ggaaccagag acaaaaatat ggtcgcaata ctggtcgata tagattgttt taaaagttat
      421 aatgataatt atggtcatct agcaggggat aaagttatag ttaaggtaac tgatattata
      481 agaagtgtat ttatagatat taccgaacac ataatcagat atgggggaga agaaatttta
      541 atattagtag attctataga atcaatcggt gttgaagaga ttgataatat agaattatat
      601 attaaaagag ttttcaaact tcttgaattt gagggtattg aacatatata ttctaaaata
      661 aaaaactata taactataag tgctggagta agtataaaaa ggtgtcgtag tagaaaagat
      721 gttgaaatat taattgaaga tgcagataag aacctatata aatcaaaaaa agcaggtaga
      781 aatcaataca ttataattta aaattgaggt gattttgttg cgaaaaaaaa taggaattat
      841 agcctctgat attgaattaa aagaaagaat agaagaatta tatagagaag atgtagaaaa
      901 tggaactata ataatcgaca tacttaacct agacttaatg gaaaatcaag gtcgtatact
      961 tgttgaaaaa ggtgcccaag ctataattgg tagaggtgga ggttatagct tagtaataga
     1021 tacagtaaat gtacctgtta taccaatgaa tatgaagtca actgaccttt taagagcaat
     1081 agaaatagca aagaaatatt ctaaaaaagt agtattgatt cttggagaca atgaagtttc
     1141 ttttgactat gttggctgga gaaatgtcat aagtactgaa ataactgaag aatggtttga
     1201 atctaagtat gagataagaa gtaaagttgt aaaatatata gaccaaaaag atgaagttgt
     1261 aatcgtagga ggaggtttgg catgttcttt tgcaaggcaa tatggaattg atagtgtatt
     1321 tgcaactgct agtgatgaat ctataagaga agctgtagaa tattgtaaaa aattattaga
     1381 tacattaggt gaagaaaagt ttaataatga agtgttacga aatatacttg atggtataaa
     1441 agatggagta attgctatag atagtaatgg cagtataatt ttgtacaatg aaagtgcaaa
     1501 aaacatgctt aaagtagaga gaaaatgtgc acttaataaa tacatcttag atgtatttcc
     1561 taaaatggag tggatgttag attgtttaca tgaaaaagag gacgtagaag atagaaagat
     1621 aagaaatata aataatctaa ttgttaatac taggacaaca ttgataaaag ttgataatag
     1681 tacttatgga gttttaggaa taatacaaga tataaccaag ctacaaaact tagaaagaaa
     1741 aataagattt gacttgaatc aaaaaggtct ctatgctaga tatacttttg atgatttttt
     1801 atttaaagat aaattaacta aagaatttat tgaagaagct aaaaaaattg gaaaaagtga
     1861 ttatacaact ttactttatg gagaaagtgg gtctggtaaa gaaattatag ctcatagtat
     1921 acacaacata agtaaaagaa aagataggcc ttttgttgct ataaattgtg ctactatagc
     1981 agaaaattta cttgaaagtg aactttttgg ctatgaagaa ggtgcattta ctggtgctag
     2041 aaagggtgga aaaagaggac tatttgaact tgctcatgga gggactcttt ttttagatga
     2101 aataaatagt ctatccttta atattcaaac aaaactatta agagtaatag aagaacgtca
     2161 aataatgaga attggttctg attatataat acctctggat ataaggatta tagcagcaac
     2221 gaatgaatct ttaaccgaaa aaatagttat gggtacattt agagcagact tattttatag
     2281 attgagtagt ttagagataa atatacctcc tttaagagac agaagagaag atataatacc
     2341 tttgtttaac aattttgtaa atgaagtatt aaaggatgat ggtctcaatg gaataaacag
     2401 tatagatgaa aattttgttt tgactaaaga tgaaatggat aagctatata attatagctg
     2461 gcctggaaat gtaagagagt taaagacaat agctcaaaaa tatgttgtga caggtaaaat
     2521 taaattgaga caagatagag attttaaaac taaaaaatta cttcctaact ctgaagtaga
     2581 caaatttaat tctgaaacga cagcaagtgt agaagtccaa gatgaatcta taaatataag
     2641 taagataaat gatggtaaga taagtataga tataaaagaa gtaaataagt atgttgaaga
     2701 aaaaataata agtatgcttt ttgctcaggg actttctaaa aatgaagttg cacaagttct
     2761 tggaataagc aggacatctt tatggaagaa atacaataaa aatatttaaa tataattaca
     2821 tataaattat tttgaagtaa tgttaatttt ttaaaacaag tgttaagaaa aattaacatt
     2881 acttttttgc aaatttttat ttttttagac aggcatatat atttataaga aaaatagtta
     2941 aaacacttaa tttaataatg aaaaaaataa taattctaaa caaaatcaat tttacataaa
     3001 gtgggattta ttggcatagt aattgcttaa tatataatgt aagagtaaat acaattttag
     3061 gggtgatggt atgagtcaaa gtatggttgg taagcatgca atgtggccaa aagaaaatga
     3121 tgttatattt agcatatctg gcagagctca agcagcagag aaggcttttg gtatggataa
     3181 tgtaataaat gcaacaatag gcgctctaat ggatgattca ggaaaattga taacaatgaa
     3241 aactgtatat gaagaatata aagcattaga taactgtgag ataggtgcat atgcagctct
     3301 tgaaggtcaa ccagattact tagaagcagt taaaaaagta tttttcagag attacttacc
     3361 agaaggacat ataagagtat tagcttcacc aggaggaagt ggtgcaataa aattagctgt
     3421 atggaactat actaatgaag gagatgaggt attaacatct gattggttct ggagtccata
     3481 tgtaagtata gctgaagagg ctaatagaaa agtagttaat taccaacttt ttgatgaaaa
     3541 tagaaggttt aattttgagt cgtttaaaga aaaatttgta aatatagcag aaaaacaagg
     3601 tagagtcttt acaattataa acacaccagc gcataatcca actggatata gtgttgcaga
     3661 tgatgagtgg gataaaatat tagacttatc aaaagaagtc gcaaaagata aagacaaaaa
     3721 gataatattt ttcgtagatt cagcatatat tgactttgca ggtgatgatg atgtatgtag
     3781 aaagtttttt aagaaatttt ctaatttgcc tgaaaacgtt ctcgtattgg ttggattcag
     3841 tatgtcaaaa ggatttacag cttatggtat gagaatggga gctataattt gtatatcttc
     3901 aagtgaagat gtagctgaag agttccatta ctcatgtgtt cattcatgta gagcaaattg
     3961 gtcaaattgt aatagaagtg ctatggcagt attatctaac attgtaaatg accctaaaaa
     4021 attcaaagaa tatgaggatg aaaaagaaat atacaaaaac atgcttacta gaagagcaga
     4081 tgtatttgta aaagaagctg aaagagtagg attagaaatt cttccatata tagcaggttt
     4141 ctttgtaagt ataccatgtg ataatccaaa agaagtatgt gaagaattga ctaaacataa
     4201 tttatttgca gttccattaa aaatgggctt aagatttgca gtttgtgcag tttctgaaga
     4261 taaatgtaag aaagcaccaa gtattataaa agaagcactt gaaagtttag aagtaaaaat
     4321 aaataattaa tttcattgaa caaattgggt ggactatgaa tttaataatt gtagatggag
     4381 ggaattttat atgaaacaat tgatgacagg taatgaagca attgcacgtg gagcctatga
     4441 ggctggtgtt aaatatgcat ctgcatatcc agggactcca agtacagaaa tattagaaaa
     4501 tatagcaaca tataaagatg caatagttgc agagtgggct ccaaacgaaa aggtagcact
     4561 tgaggctgct atagggggtt caattgcagg agcaagaaca atggcttcta tgaaacatgt
     4621 tggtttgaat gttgcagctg atccaatatt tacttatgca tatacagggg taaatggtgg
     4681 tatggtactt attacagcag atgagccggg aatgcattca tctcaaaatg agcaagacaa
     4741 taggatgtat gcaaaatttg caaagatacc tttatttgaa ccatcaacta gtcaagaagc
     4801 taaagatatg ataaaagaag cttttgaagt tagtgaaaaa tacgatacac cagttttata
     4861 tagagttaca acaagacttt gtcattcaaa aggattagtg gagtgctatg atagagaaga
     4921 agttgaaatt aaagagtatg ttaaaaatgc taagaaaatg gtaactgttc cagcaaatgc
     4981 tcaaataaga aggggcgttg ttgaagaaag aatggaaatt cttaaaaaat tctcaaatga
     5041 aactgattta aactactatg aaataaatga tacaaaaata ggtgttatag catctggtat
     5101 gtgttgtaat tttgccaaag aagtttttgg aaaaaatgca tcatatatga aactaggatt
     5161 tacaaatcca ttaccttatg aaaaaataaa agaatttgca gaaaaagttg ataaaatata
     5221 tgttatagaa gaaaatgacc catttataga agaacaaata aaagcatatg gaatagattg
     5281 tattggaaaa gatgtaatac caccatatgg agaaatgaca ccagatgttt taagaaaagc
     5341 tatatttgga aaaacaaatg atactataga atacaaatct gaattagtaa caccaagacc
     5401 accaagtttc tgtgcaggat gtccacatag aggattcttc tatgaacttg gaaagagaaa
     5461 aaatttaata gttggtggag atataggttg ttatactctt ggatttgctc ctccatataa
     5521 tggaatagat tatgttgttt gtatgggttc tgcttttgga actgcacatg gagctcaaaa
     5581 agttcttaat atgaaggatg ataatgaaaa aagattggta ggtgtacttg gagactcaac
     5641 attcttccat acaggaataa atggactttt agatgttgtt tacaatagag gtaattctat
     5701 ttcggttata ttagataata gaataactgg aatgacaggt caccaagaaa acccaggttc
     5761 aggatatact ttacagggtg ctaaaactaa agaagttgat atagaaggat tagttaaagc
     5821 ttgtggaata gaacatgtaa gagttataaa ccctaataat ttaaaagaag taaatgaagc
     5881 actagattgg gctttagcta tagaagatga accatcagta ataataacaa gatggccatg
     5941 tgtacttaaa aaattctcta aagaagatat agaagaattt aataatccat ttaagactaa
     6001 gtgtaaagtt gaccatgata aatgtatagg atgtaaatta tgtttgaaaa ctggatgtcc
     6061 agctctatca tttgataaag agaataagtt atcaaatata gatagaaatc agtgtgtagg
     6121 atgtggagta tgtgcacaag tttgtccaaa acaagcaata gttaaggagg aaaaataata
     6181 tgactaagag tatacttttg gtaggtgtag gaggtcaagg aactatactt gctagtaaat
     6241 tattaactat gggattgatg gaagctggat atgatgtaaa gatgagtgaa atacatggaa
     6301 tgagtcaaag aggaggttca gtttcctcac aagttagata tggagattgt gttcattctc
     6361 cagttataga aattggtgga gcagatatat tagtatcttt tgaaaaaatg gaagctttaa
     6421 gatggttcaa ttatttaaaa ccagaaggaa aagctttagt gaataatcat aggatagatt
     6481 ctatgactgt tttaatcgga ggagctgaat atcaagaaag tgatatagat gcagagttaa
     6541 gtagattaaa tgcaaaagtt ataaatgcag ctgataaagc tgaagaacta ggaaattcaa
     6601 aaataatgaa tgttatttta ttaggatgtc tagttaaatc tatgcaatta gagtctattg
     6661 attgggaaaa aattataagt gaaaatgtta aaccaaaatt tgttgaatta aacataaaag
     6721 ctttccatga aggtatggaa atggttagca aatagtttat aatagatata aatagactat
     6781 agaaattaag gtgaaaggaa gaaaattatg acttacagaa tattagccat aaatccaggt
     6841 tctacttcta caaaaatagc agtatatgat ggagaagaac aaattcttgt gaagacgata
     6901 gaccatccag ctgaagagat tgcaaaatat aatactatac aagaccagtt tgaaatgcgt
     6961 aaggaagcag ttttgaatat tcttaaagaa aatagtatag acttaaaatc tcttagtgca
     7021 atagtaggaa gaggtggagt tttaccacca gtaaaatcag gagcatattt agtaaatgaa
     7081 gaaatgattg atgtactaag acatagacca gtacttgaac acgcttccaa tttaggtgct
     7141 gttgtggcac atgcaatatc agaacctctt ggaatcaact catatattta tgattctgtt
     7201 gcagtagatg agcttataga tgtagcgaga atatctggac tttgtggaat ggatagatca
     7261 agtgcagggc atgcattaaa tactagagca atggctttaa aatatgctaa ggataaagga
     7321 aaagattata agagcttaaa cttaatagta gctcacattg gtggaggagt aagtatttat
     7381 cttcatgaaa aaggaagaat ggttgatatg ctatctgatg atgaaggacc attttctcca
     7441 gaaaggtcag gaagagtacc tgctacaaaa ttagtggctg cctgttattc aggtcaatat
     7501 tcagaaagag aaatgactaa aaagataaga ggtaaaggtg gtatagtttc atacctaaat
     7561 actgtagatg ctagagaagt tgaaaaaatg atagcagaag gaaatgaaga agcaaaaatt
     7621 atttatgaag caatggctta tcagttagca aaaggtattg gagagttagc aactgtagta
     7681 gatggaaagg tagatgctat aattataaca ggtggaattg catattctga aatgtttact
     7741 tcaatggtta aaaagaaagt tgagtttata gcaccagtag aaattatggc aggagaaaat
     7801 gagttggaat cacttgcttt tggaacttta agagtactaa atggagaaga agaagctaga
     7861 atttatagtg aaaattaata agaaaaacta aaaatgttta tatactaaaa aagtggtata
     7921 tccgtttttt atacacgaaa gcttagagta taggtgaaaa tgtataaaat ttggattgcc
     7981 actttttata attaaaattt tatcttaacc taataaataa ttctgtaaaa tttcgtattt
     8041 atatatatat tttattggta tttattgtta ttattattat gaggagtgat ttttgatggg
     8101 aaaaataagt ttaaaaagta tgataagatt attttcaggt ttttttatat ttgcaatttc
     8161 ttctgtgctt atgattaatg cacatgtagg tcttatgcct tgggatgtac ttcaccaagg
     8221 tttatctata aagctaggca taactatagg tcaggcttct ataatggtag gagttgtaat
     8281 tgttatattg gatgcagttt ttggtgaaaa tataggatgg ggtactttac tcaacatgac
     8341 atttatagga atttttattg atttagtaat attttctgga gtgatacctc atgcaagcaa
     8401 cacttatata ggtgtgttta tggttgttat aggaattatt ttggcagcta tagcaagttt
     8461 tctatactta ggtgtttgtt taggaagtgg accgagagat gggcttatga ttgctcttca
     8521 gaaaaaaact aataaatctg tacgtcttgt tagaacaatt cttgaaatac tagcattagt
     8581 tgttgggtgg ttattaggag gttctgtagg tattggtacg cttgtaagtg ctttaggttt
     8641 aggatatgta ttacaaatag tgtttagaat atttaaattt gacacaaaat tgttgaaaca
     8701 tagatttatt atagatgata taagagaatg gaaggaaaag aaaagcaatg aacataaatg
     8761 taaatcaagt attgtaataa aaaatgagca gaattaatta tgctaaaata tatttgtagt
     8821 ttatctaatt tatagtaaag tcaaggagat tttgaaatgg aattatggga tttatataat
     8881 gctgatggaa ttaaaacagg aaatgttata aaaagaggta attctattga agaaggttat
     8941 taccatttgg cagttgaagt gtggatttta aatagtaact ctcaaatttt aattcagaaa
     9001 aggtctaaaa gtaaaaagac attgccaaat atgtggggaa tgacaactgg atgtattgtt
     9061 tctggggaag acagtttaga aggagcaatt cgcgaagcaa aggaagaaat agggatagat
     9121 ataactaaag atgagatgaa agtttttaga agtatgattc atgaagatac tctttgggat
     9181 gtatacttag ttaaaaaaga atatgatata tccaaagcta ttttacagga ggaagaagta
     9241 agtgatataa agtgggtaag cacagatgaa ataagacaat tactaaaaga gggtttgttt
     9301 tttgaatatc cagagatata cgaattgcta tatgagatag ataataataa attaaatata
     9361 tgatgtagag cagatttcag tataagtaag attatttaaa atgtatcttc tctaaatttt
     9421 aatattgttt tatgaaaatt aataagttat ttttagtatt tttctacttg ttttttgaga
     9481 ttaaaataaa aagggcagtc attataaagt cataaaaatt tggggaggat ttaaactatg
     9541 gaagataaca aaggattggc acgtgacagt tcaaaatata aaattgtttc tatatctgtc
     9601 gtagtaatta ttctaattac attttggtat atgttaaata tggtgctttt aacatttatt
     9661 atgactttta ttttctataa cttgcttgta gccacaagaa aacgaattaa gaagttttct
     9721 tctttaaata ttccagattc attaataatt atagtgttat atgcactatt tgctatattg
     9781 ttagtattga ttagctatgc agttgtacca ataattatag ttcaacttac agagttgagt
     9841 agggttttta gtgattttga tgtaaatcaa tttgctcaat ctttaggacc aaaattgtat
     9901 cctattgtct caaagctgga ttttaataaa tatatttcac aagcagggct acttatagca
     9961 tcaacagcaa caaaagttgg aagttttgga gtaaatatat tattggcatt tttactgagt
    10021 ttactccttt tacttgaaaa aaatgaaatt aaaaattttg gagataaact ttctgatagt
    10081 aagatttctt ttatttataa ttcgcttgta ttttttggaa agagctttgt taaaaatttt
    10141 ggtgaagtaa tgaaagtaca agttatgatt gcctttataa attctgttgt ttctatgatt
    10201 tttttaggtt tcatgggatt ccctcaaata tgggctttag gatttatgat ttttgtgttg
    10261 ggtcttattc cagtagcagg agttatagtg tctttgattc cattaactgt aatagcattt
    10321 aatacaggag gaataacaaa ggtttttgga gttttactta tgatttgtat agtgcatgca
    10381 gtagaaacat acatattaaa ccctaagttg atgtcgaata gaactaaact tccagtttgt
    10441 tttgttttta ttattttact tgtaggagaa cattatttag gtgtatgggg attactaatt
    10501 ggagttccaa tatttatgtt ccttatggat attctgggtg ttaaatttac ttctaggtaa
    10561 tatattaaaa taataattta tctttatata taaattatga attgttttaa aaaagaacct
    10621 ataaatagac atcattaata aagacatcta tttgataggt tcaattttgc tttgtataaa
    10681 tactataaaa ctaaataaaa agaaatttat ttcatttcaa aagtttcaca acttgcattt
    10741 ctttcattta tatgaacttt ttcagctttg caaagttcat ttttattgta tttacatttt
    10801 acagcttcac aatgaatgtt atctgtatca acttggttag tttcattaga acgactagtt
    10861 ataccagatg tatatttgtc ttcaaaagaa gcacaataag tattttctgt agtagttgct
    10921 tgcattccat ctacttttat actaccagca taacaatatc cactagtatt ataagcacaa
    10981 ttagtagcag cacaatttaa attattattt ttcataaata acctcctaag ttttatttaa
    11041 acttattatg tgaagttatc taaattttat acacttattt acaattacat ttatcacatt
    11101 taacaactac atctttggaa gtttcccttc ctaaattttt attaacatga tacacatcaa
    11161 cattttcagt tgatccacat atagaacatt taaaattgtt ttctagtagg gttctttctt
    11221 taaataactt ccaatacata gaattgatgt aatcattata atttaccttg aggcatgtct
    11281 caagaaagtt tatgtataga ttgtctaaat ctcctgtatt taattctgta tattctggtt
    11341 caaaatattc acaacatcct ctaaaatcag gctttgtatc tgaaataaca catctaatat
    11401 catatctata gtctaggcta ggccccttaa atgaaagatg tgtgcaaaaa gaacaagatg
    11461 tttctggtag atttttttca agttcatgta atttattatt cataagtatt cctccaaaaa
    11521 atttatttta agtatataaa cgaatagatt attttaaaac ttttatttat ttatattaga
    11581 gatttaggat aaatattcca ataaggagta aatacttaag tatttattac tgtattaaat
    11641 aaaaaagagg caaatctaac ttgagattgc ctactttttt atcatttttt aataaaattt
    11701 attctaagta ttattttatt tagatttagt cagccaattt agtatctgct ttgcttttta
    11761 actttctacc agagcgtaca gttaaaatag catataatac agtaaatatg gcagctaaaa
    11821 catatgatat atttatgttt aagttaaatc caatttgagc attaaatata taactgaata
    11881 taacaaagga ataaaacata ccaggaatta agcttataat ataattcttt tcttttatta
    11941 taaggtaaac tgtaatcatt gcaaatgcaa atatggcaat agtttgatta ctccaagaga
    12001 agtatctcca caatatatta aatccagaag cactaagctt tgcaaatata aggatggcta
    12061 tgacaggaat aaatatacag attgacaata taacacgatg tttcttttcc ttttggtcat
    12121 aatgaaagta atctgcaagc attaatctta aagatcttaa agctgtatct ccagaagtta
    12181 ttggtaaaac aattacacct attatagcta tgataccacc aatagaacca agtaaatctc
    12241 ttgcaaccaa acctattaca tcaggagaac ctactaattc tttaggtatt cctttattgt
    12301 aaatacccat agcagctgca gcccaaatca ttgcaattaa accttctaaa atcatcatat
    12361 tataaaaagt agtttttcct tctttttcat ttgaaactga tcttgctata agagtagctt
    12421 gagttgagtg gaatccagaa acaataccac aagcaactgt aacaaagaat gtaggaataa
    12481 gaggaatacc atcaggatga atacctttcc aatttgcaag acttaaatta gctaaatcat
    12541 aaccttgtgt aaagattcca attccaacac cagcagcaga taaaagtaga atagctccaa
    12601 atattggata tacctttcct atgattttat caattggaaa tagtgttgca cacaagtaat
    12661 atagaaagat aagtccataa actatccaga caactggatt atttatagtc gatttcatat
    12721 ttaaaatttg agtaacaact aaatccccag gtgtataaat aaaaactgca ccaactaaaa
    12781 gcattaaaag acaaaggaat atattataga cttgaaatac tttattacct aaaaatctac
    12841 tgataagaga aggcatttgt gctccctttt ctcttatact aatcattcca ctcatatagt
    12901 catgcaaagc tcctccaaat acacagccaa taggtattag tatgaatgct attggaccaa
    12961 acaatatacc ttggataggt cctagaattg gtcctgtacc agctatatta agcaactcaa
    13021 ttagactatt tttccatttt ttcattggga catagtctac tccatcactt tgagcaagtg
    13081 caggtgtttt tctgtcatct gggccaaata ctttttcaca ataagcaccg tataaagcac
    13141 ctcctattag taatatagcc aagccgatta agaatgttgt cataatatca ccccatttat
    13201 aaaatttttt ggtctttgta tacatattat ctaaatgtgt agatttatat tgaatttaat
    13261 tctggacaac ctacaaattc tataaagtat tacaactaat ataatttcag aaataaatac
    13321 atatacataa ccatgtactt agtatacagg tatttggtaa tattttcaat gttatagcaa
    13381 taaagaatat aaacaaaatt ctgataatat tttacaaaaa aattaaaaaa tataaaaatt
    13441 agtttaaata gatggagaaa aaaggaaaat caattgagaa tataaatgtc tatatcttaa
    13501 caatattaag aggtttaaaa ttcttgaaga aagaatagtc aagtgatata atgggtttac
    13561 aggtgtatat acactagtac atatagaaag cggtgcaatt atggataaag atttaacata
    13621 tcaaatcaaa gagctaaaga aagaaaaaaa tgcaataata cttgcccatt tttatcaacc
    13681 accagaaata caagaattag cagatgcagt tggtgactca tattatttaa gtgagattgc
    13741 tagagattgt aaagaagaag tggttgtatt ttgtggagta aggtttatgg gagaaagtgc
    13801 taagatatta tcacctgaaa aaactgtatt gatgccagta agtaacgctg ggtgtgctat
    13861 ggcagatatg gtagatgaag aaggcgtgat aaaattaaaa caacagtatc caaatgcttt
    13921 ggttgtatgt tatataaact ctacagctaa agttaaggca cattgtgatg tttctgttac
    13981 atcttctagt gcaattaaaa tactagaaaa catagataat aaagaaatta tattccttcc
    14041 agataaaaat ttaggaggat atattgcaga acaatttcca gataaaaact ttatattttg
    14101 ggacggatat tgtaaatacc ataacaatat aagagctgaa gaaataattg agttaaaaga
    14161 taagtataaa aatgcagaag tattagtaca tccagaatgt aaaaaagaaa taagagattt
    14221 aggagattat gtaggtagta catcaggaat aataaaatat gctactaata gtaaaaacaa
    14281 agactttata atagcaactg aagaaggtat attacatgaa ttaaagaaaa ataatcctaa
    14341 taaaaacttt tatattcctg gtggaaaaat actatgtaca gatatgaaaa agacaacatt
    14401 agaaaattta tatagtacac ttaaaaatat ggaaaatgaa gttattgtag aagatgaaat
    14461 catggaaaag gctttaaatt cactgcttaa catgcacaaa ttagcagaag gatagaggga
    14521 tagacttatg aatttagaac aagatgtttt aatagttgga agtggagttt caggattata
    14581 ctgtgcatta aatttagata aaagtttaaa tgtgctagta gtatcgaaat caacaattga
    14641 aaacaacaat acatatcttg cacaaggtgg aatttcaact gctagaaata ttgatgatat
    14701 agaatccttt gtagaagata caatgaaggc tgggcagtat aaaaatagag ttgaagcagt
    14761 tcaagtactt gcagatgaat ctatagaaaa tgtaggtcag atagtagaat atggtatgcc
    14821 tttagacaaa gaaaatggag agatagatta cacaagagaa ggagcacata gtgtaaatcg
    14881 aatagttcat agtaaggata atactggaga agttgttttt aaaactttat taaaagaagc
    14941 taaaactaga gaaaatataa ctcttataga agatgcatat cttttagata tattaaaaga
    15001 tggcaataaa tgtataggag ctagaatttt taaatctaaa aaagagattc atgttttttc
    15061 aaaaatagta gttttagcta caggtggaat tggtgggctt tttaaaaatt ctactaatca
    15121 aagacatctt acaggtgatg gaattgcaat agctttaaga aataatataa aaatagaaaa
    15181 tttagaatat attcaaatac atcctacagc cttttatgaa gaaaataatg aaggtaggag
    15241 aatgcttata tctgaatcat taagaggaga gggagctaaa cttttaaata aaaataaaga
    15301 aagatttgtg gatgaacttc ttcctagaga tgtagtttca aaagctattt ttgagcaaat
    15361 ggaaaaagat aaattacctt atgtgtattt agatgcaaca catttagata gtgaatattt
    15421 aattaataga ttttctttta tttataatga atgtttagct agaggaacag acataacgaa
    15481 agaatgtata aaggtatctc ctgcacaaca ttattttatg ggcgggatac atgtagattt
    15541 ggattcaaaa acatctatgg aaaatttata tgcagttggt gaaattagtt gtacaggtgt
    15601 tcatggagcc aatagattgg caagtaattc attacttgaa gggcttgtat tttcaaaaag
    15661 agcagctaaa aatataaact cagttataga taatgtaaaa gttaagttta tagatgtacc
    15721 agatatggat atagatatag aacaggtaaa aaaagaaaat aaaatattag tcataaaaga
    15781 aattgaaaga acaagtgagg attttggcga tgaattattt gattattgat agaatgatac
    15841 aggatgcttt gatagaagat gttccaagtg aagatataac aactaattct attgtggatg
    15901 aaaattctaa atctactgta gatttaattt gcaaacaaga tggtataata gctggcttag
    15961 gtgtatttaa gagagttttt gagatacttg gtgatgtaga tgtaaaatta tataaaaatg
    16021 atggtgatag agtaaagaat cgtgaaaaga tagctgtatt aacaggtagt actagaaact
    16081 tacttgtagg agagcgtgta gcattgaatt atttacagag aatgagtggt atagctacta
    16141 ttacaagtag gtatgttgaa aagcttgaag gtacaaatac aaaattatta gatagtagaa
    16201 aaacgatacc taatcttaga atattagata agtattctgt caaagtaggt ggaggatgca
    16261 atcatagatt taatttatct gatggaatat tgcttaaaga caaccatatt ggggctgctg
    16321 gtagtgttaa gaaggcagtt gaattagcta gaaaaaatac ttcttttgtt agaaagatag
    16381 aagtggaagt ggaaacttta gacatggtaa aagaagcaat agaagctaat gcagatataa
    16441 taatgttgga taatatgagt ttggaaatgg caaaacaagc agtagatatt ataaatggaa
    16501 gagccattgt agaattttcc ggaaatgtaa atttagatac tatagaagat ataggaaaaa
    16561 ttggtgtgga tgttgtttct gtaggagcgc ttacacattc tgttaaagct ctcgatataa
    16621 gtatgaagaa tttgagaaat atataaattt tatattgaaa taaacttaat taaaatagac
    16681 tccttaaatt tgttttataa atttaaggag tctatttt
//
LOCUS       DAELJO010000074        16334 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELJO010000074 DAELJO010000000
VERSION     DAELJO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.074"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(16..1614)
                     /locus_tag="KPB88_002024"
     CDS             complement(16..1614)
                     /locus_tag="KPB88_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0054302.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKPNIEGTVDTSKAGTYVLNISAFDNFGQKCEINVNVKVIVNTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKRALRELDSLAGMANAWSKYMNEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPINNKSIYTSKDAKEVAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNRGEVNATMEFLNS"
     gene            complement(1833..3434)
                     /locus_tag="KPB88_002025"
     CDS             complement(1833..3434)
                     /locus_tag="KPB88_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG0054303.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSNKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDINRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(3802..5682)
                     /locus_tag="KPB88_002026"
     CDS             complement(3802..5682)
                     /locus_tag="KPB88_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG0054304.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLRGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIVNKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(5892..11855)
                     /locus_tag="KPB88_002027"
     CDS             complement(5892..11855)
                     /locus_tag="KPB88_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG0054305.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDNNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKILTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKIDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(11979..12064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(12236..13639)
                     /locus_tag="KPB88_002028"
     CDS             complement(12236..13639)
                     /locus_tag="KPB88_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG0054306.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(14101..15996)
                     /locus_tag="KPB88_002029"
     CDS             complement(14101..15996)
                     /locus_tag="KPB88_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG0054307.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         4584 a         3087 c         1799 g         6864 t
ORIGIN      
        1 atattaatat aaaatctagc tatttaaaaa ttccattgta gcattaactt ctccccgatt
       61 agaaacatac attccaaagc caaaactata aaactcttcc tttaacatat tatcattata
      121 cttagaagac ttcttccata aatcaaatac agatttagca acctcttttg catctttaga
      181 ggtgtaaatt gacttattat ttataggtaa atatgcaata ttttcatcag ttctcattcc
      241 aaacccagaa aatacttcag ctgcattttt cccatcaatt tcatgagcaa atatcttctt
      301 ctcattcata tacttagacc atgcatttgc cattcctgct aaagaatcta attctcttaa
      361 agctctcttg cctttttctt tcctatatga attaactaga ttatacattt cattagatac
      421 catagcctta aactcataac tattaggatc tttcgtactt gtatttacaa ttactttgac
      481 atttacatta atctcacact tttgcccaaa attatcaaaa gcacttatat tcaaaacata
      541 cgttcccgcc ttgctggtat ccacagttcc ctcaatatta ggcttcaaat catttccata
      601 ataatcagta gcaactatat tcaacatact attatcaaac ttatccccta cagaaatctc
      661 tacatcatta gcagtgattg taggcattgt atttttatca gatgcagaac taatacattg
      721 gtcaacaacc ttcttatcca ttccacctaa agaagtaact tcatcagcac ctctaagcac
      781 actcttatca cttccatcat taacaagtac tataggtttg tccttagcca acggagaacc
      841 agccacagca tcaactaact tatacccttg agaaacataa aacttctttg ttcctttata
      901 aaagcgttgt ataaccttct tattagtctc aaacctatcc ttaccagcaa tcctaacaga
      961 attagtctta ctcacaagtt cattactcat tatctcctct gacccaagag aataacattg
     1021 aacgccatca accttaaatg gtatactctt tccatcagta agtatcacag gaactccatc
     1081 tctagcagct acagaagata cactcattgc atcagcctca cctgtgtatc cattcacaag
     1141 gaaaacctta tcgccatcat ttactggttt aatagctgaa atctccttag ctataagata
     1201 gctagtcttt attctatctt ccccaccaat tcttttaact tcaatccctt tattcttaag
     1261 ttgattttct acagatttac ctattgtatc ctctgtacct ataacataag cattcttaac
     1321 atcctttagt cttttttcaa catctgttgg tatcttatct ctttgcacca acattatagg
     1381 agcttttaca actccagata atccacttgc acttaatcca tctactatag aattatcagt
     1441 atttactaat atgactgtat catatatttg tttatctgct atcaatgcag aagtctcata
     1501 tctatctttc ccttgtatct tttctatgct tgaaagtgca ttgatatttg gtgctcctgc
     1561 cattattaat gatattgtca atcctattga taatactctc ttatttacct tcatcaaact
     1621 cctcctacta atttttttat ataattcagc taatcataaa ttacatcctc agtagtgatt
     1681 ccctcaaaat ctataaacta ctaaaacaaa aaacacctaa aataccataa cctgtaaatc
     1741 aataacaaaa cagattacaa aaagtacttt agatgtgatt ttcattttat aatatcagta
     1801 aattagcaaa aaaacaagat ttatggaatt ttttacaagc taccttcatt gccatttaaa
     1861 aactcctgag tagcatgcac ttgaccatca gataatatat aaagcccaaa tccagtacta
     1921 taaaactcat tactaagcat gtttgcattg tactctggag atttcttcca cacctcaaat
     1981 atagcctttg ccaagtcttt tgcatcttgt gtggtttgaa cgttcttaga atctatataa
     2041 atatatgcta tattttcctc acttctcatt ccaaactcag aaaatacttg tggagcattt
     2101 tttccatcta tataatgtgc aaatactttt ttatccatca tatacttaga ccatgcattt
     2161 gccatacctt ctagcctact agatactacc aaaggctctt tgcctttttc ttttctatag
     2221 gaattgatta agttatacat ttcagtagat accatttttt taaattctgg gctgttgtaa
     2281 tcatgagatt tgtcatccaa cacttttatt tctactcttt ttcctgtgct ttttccccat
     2341 tcgtctgtag cttttaatgt caatatgtat gtacctactc tatttatatc tataaaccca
     2401 tcaacttcta ttggtaatac cttcccagtg ttatctttag ccactatatt taacttgcca
     2461 gtgtcaaact tttcaccctt atatacctct gtacttccaa ctgtaattgt tggtggttgt
     2521 ccatttgact ttgaagcact tatacattgt tgtattacct tctcatctat ttcacctaca
     2581 gaggttatat tcttagctcc ttctaataca gttttattgc ttccatcatt tactagtacc
     2641 attggagagt ttttagtaag tggtgcagct gctattgcat ctactaattg gtatccatct
     2701 gatacataga atccatctgc actattaaaa aagtagtcta ttacgttttt gttagtttca
     2761 aatcggtcag ttccttctat tcttactgag tttgtatttt taactagagg attactcatt
     2821 atctcctctg aacctataca atatgattga atatttttta catcaaaagg cacactcttt
     2881 ccatctgtaa gtatgattgg agctccatct ctagtagcta ctgatgatac actcattgca
     2941 tctgcttctc cactatatgc attagttaat agtactttat ccactttttt tatagttgct
     3001 atttctttag ctattaaata gcttgttttt agtctatctt ctccaccaat tcttttgact
     3061 tctatatttt tagaatctag ttctttttct actgatttac ttatagtatc ttctgtacct
     3121 attatatatg cttttttgat attttttaga catctatttg tgtctgctgg tatcttattt
     3181 tgttgtgtaa atagtattgg agcttttgta gctcccgata atccacttgc acttaatcca
     3241 tctgcaagtg atttttctgt atttactaaa attactgttt cataagtctg tttatcagct
     3301 atctttgctg ctgtttcata tctatcttta ccgtatattt tttctactac tgatgatgca
     3361 tttacacttt tgaagtttac taagattaat gatactgcta gacctaatga taatattttt
     3421 ttgttcattt tcatatcccc ctgatgattt ttatatttta tatttatatt gtgattattt
     3481 tccatttatt ttctctttat tttccatata ttatagagta tatatgatat gaatatatct
     3541 cgtctatata ttagtgtttt attttattaa atttaaaaaa ttatttttaa cttctttatt
     3601 aaacatattc aaaattgaat ttgtatttat atatataaaa gatatctgct ttatttaaaa
     3661 ttaaacttaa tatagctcaa attattatac attttaacct caatattatt aaattgttaa
     3721 gataatgaaa ataaaataag aatgcattta aatatataac tttatatcta aatacattct
     3781 tattttctat ttacttaata tttatttttc aattaaactc ttaatcttat ctattacaga
     3841 tgaggctatt cctccaccta cttcaaacaa ctttgatgaa tttttacctt ttaatgacga
     3901 ttcttgtgat ttatctaacg tatttgtagc aagtataact ggtgaattgt ttttagctgc
     3961 taatggtcca actgctaaag catctataag tttgtcttga tttgcaacta caactccatc
     4021 tagtttagat tgagagtaga atttatctat tactttggca ttcgtctctt gtctagtttg
     4081 acctgccacc ctattgtttg atatatcttt gtttactatt ttgccaactt gttcaatagc
     4141 cttgttagat attttttcta ctccaccaat aaaatatata tttgacacct tatttgattt
     4201 aataaattgt tcagcttcat tatctacacc atctttttgt gtaagtacta ttggagtttt
     4261 ttctttacca gccagtgatg ctattgataa tgaatcagct tctccatttc ctgctcctat
     4321 atacaacttg cttacattat ttgtcttatc tatttctttt gctatattaa tagaagtttg
     4381 atatcttgta tctccaccta ttctttgtac tgatattttt gaatttacag ctttgatagc
     4441 ttcaacaact gaatttggca ttgagttgtt tccacctata acaactactt ttgatggatt
     4501 tagccttttc aattcttcta cagttttttg aggaagcttt tcatttgatg ataaaagaat
     4561 cggtgagttt tttacactag caagtggtgt agctgtaagc ccatccacca tacttttatc
     4621 ttctccattt actattacaa ctgtatctga ttttgtccaa ccactttgag atattttgat
     4681 agctgtctca aatctatcat caccttttaa ttttttgact gtgacattag atgtagaatc
     4741 acttgaattt cctcctccag agcttccacc actagatgag ccgcctgaat tacttcctcc
     4801 accattattt ccaccactac cattatctgg tggtaattca tccggttttg ataattcatt
     4861 ttcaactatg tattctaaac tttttggaag tgtaacttca acatatttgt ttattgtaac
     4921 ttttcttccc ttagttgttt tttgtgctaa tccaagtttt aatattacat ttgaaccact
     4981 ttcttgtgtt tctttactaa caatagaata gtttatccta atattgttta ataaatattc
     5041 aagtgcttca cttttctcta ccttatactt ttcagggtca tttttactag aaccagttcc
     5101 cccaattggt tttacttcac tctcattaat aagggtaaat attaatcctt catcagagct
     5161 tatattttca taatccactg ttttgttgtt atatgttaca atttttaaag ctggtttatc
     5221 gtttgttcct tttcctaaaa ctttatagtc agcatttgag ttacttccag atttttttac
     5281 agatattctt cctatattca atctatttcc gtctagtaag tcttcatttg atgtaacata
     5341 tatatctaat gtagttgtat tacttgattt gttgtattct gctctacaag tagcatttgc
     5401 atttccactt tttatctgag attttatatt atcagaccaa gtaatctttt ctttctgctc
     5461 attatctact acttcaacct caccttgtac ttttatactg gcttgaaatg aatatactat
     5521 gttttctact gagtccacag atatatctat agtatttact ttctcatacg tttttgttcc
     5581 tcgtaaaact ggagctgcat atgcacatgt agcactacca gttacagcta ctgccatagt
     5641 tgatactgct atagttctta taaaagattt tcttttactc attaacaccc gtccttatct
     5701 atcattaact aagtggataa atttacttat ccacctagct tttatattta atttataaat
     5761 taccctaaac ttatagttac atttatatac tttactttta atatctacta ttttaaaact
     5821 attctagtac ctaatcttat tagatataca atgttagtgt tagtattttt acgtttttag
     5881 agttccacct actatttatc tctaacaata ttacccttta ctttattgct tacagttata
     5941 accttcttat tactgatatc aatagcattt cttgtagctt ttctagcttt actattacta
     6001 tcagtaagac ttattgtctt taactcatca taattcttag ctcctacagg tacatacatt
     6061 gtatcactta caaatctttg acctactgga gtatctcctt gaaggtcatt gtatctatat
     6121 agttcagcct taaccttatc acttaaagaa tcaaagtttt cttttggcat atagtaaacc
     6181 catgtacctt tactgatttc acctagttca cctttttctg gaacttctgc aaataaaagt
     6241 gcttctccag atatagtctt attgttttta tctataagta gtggtaaatt aaatgctgga
     6301 tttccgctgt attcacctgt aataactatt gaacctgctg gaatcttacc tgtttcatct
     6361 tcacttgtgc tagagtcatc actttgatat acataatctt ctgctaactt tccaattcca
     6421 ttagtacgag tttggtctgc attattcatt gtgccaattt ctatattatc acctggaggt
     6481 cctattatat ctaattctgc aacttctata ttagacttat tatttgagat tagttttaca
     6541 tatctagctt ggtatgtcca aagtttacca tctttattaa atgtaactct agcttcatta
     6601 tcttggtctt ttcctccacc aactaaatct tgtccatatt caaaagttcc ttgacttaca
     6661 tcttcccagt tctttttatc attacttact tgtatcttgt aattagttaa aggattgtat
     6721 gttgtgctag tattctttct aaagttaaat aagcctttta atgagaactt cctagcttct
     6781 gtctctggtt ttgtatattt tagtcctact ataggtaatg tttctttcat gtctactatt
     6841 atatatggat tttcttctac atttatatca gggtcttttt gagggtcttt gttccactct
     6901 gctttactta ttatctttcc tttatatgta gtacttgcat cattatcttt tatattatca
     6961 atttcaggat tttgtaatgg tccatgtgag tcattttcag ttctaacatc accgtcagaa
     7021 acagtatttg ttatgaatga ccatgctgat ttatttattt tcccttcatc tgataatttt
     7081 acagtaccaa cttcaaattc ttctgttgga tttaggtgat aatcatatgc aactacctta
     7141 tatgtaaatg ctctgttatt aagaggagat atattatcag taaatttagt cttactattt
     7201 ccttttgcat ccacaaatcc aactgttctg tattttacct gtgttccatt ttcaccatct
     7261 aaataatttc catcacttct tattatttcg tatcctagta tcttatcttt atccttatct
     7321 acatctaggt ctatagatat tttattagat ttaactagag aaccactttg tattccatca
     7381 gaaaaactag cacttacttt agtatctttt gccatactag ctatattttc atttaaaatc
     7441 tttcttctag cttcgtcatt tacatattgt atctttcttg attctttttc gtagttctta
     7501 ctatccatta tactgtttaa ttctggtgta atttctactc cccatgcctt aaagtaatct
     7561 cttaaatctt tttcaacaac acttgatgcc attgctacta gcatttggtc ttttggtaaa
     7621 ctattaattg ggtcactact tgaaagtgct ctgtattttc ttgaaatctt tgcatagaat
     7681 gtatcattac ttaaatcagc atctgagtta ttctttaaca ttgaacccgt aaaatcatca
     7741 tcataagcta gatgtaattg ccataacatt ccaagccttt caaatacatt gcttggtata
     7801 cctaagctat ttgaagttac atgctcatat atcttatcca ttgttgaacc ttcaagtctt
     7861 gaatgtgtct tatcatcaaa ggtctgagca agtaaagcaa gtacattgtt actagtttcg
     7921 ctatatgtca tctttccaat atctgtaaca tgtcctatct catgacctat tccccatcca
     7981 aataagtttc ctcctgtagc ttttccacta tcatctattt ggaaaggtac tccattcata
     8041 agccctggaa ctgaaccata ttctactcct acatgtaatc cagaagcata cataaatgct
     8101 ccaataaaca ttctttggta ttttatgttc atacgagatt ttggagccat atcattttta
     8161 gttaagttaa attcattatc atctacagtt ccattaccat ctaagtcttg tgttttaaat
     8221 actcctcttt ctgagtaaac taaatccatt atttgttccc atgcaagcat actattatat
     8281 aatctatcta cttgtgtatt taaatcagca tttccttttg atatcccttg atatacagct
     8341 gttgcaggaa catttaatgt tactttatca ctttcaatat ctgtagtgtt taacacacca
     8401 gttttttctg aatatccata tgaattacca acatttgttc ctggataata tggttctcct
     8461 tttacagcat ctttatattt agctaattct tctatatatg cttttatctt tttcttagat
     8521 tcagaaactc ccgcaggact cttatcattt attgttccat ttagattaag atgtggtatc
     8581 ttagtagctc ctgctacacg tacttttata tctcctccac ttggagtatc ttcagcgtat
     8641 cttatgtata aacttcctcc acattctata tctaaactat gaagtttgct taattgaatt
     8701 acattttttc cagtgttaag atttatttct ccactcttat atgcaccaga ttctccatag
     8761 tactgtgtat atatcaactt aggagttttt ccacttttac ttccaacata tactgtgatt
     8821 tcatctcctg ctctagcagc tactcctaat gcctgccagt cattttgcat acctaaatta
     8881 ttggaattta cagtatactg tccattttta tctttatatt ttcctaattt atttatttct
     8941 tgatttacag tcgttacatc agtaagtgtg gctctatcat tatataagtc ttgagctaat
     9001 tttaattctc tctcaataac tgtcttatat ggatgatatt ctccactagc tttatctgga
     9061 gtatttaatc tatcttttaa ttcagttatt ttttcttgag ttacatcttc ttttaattca
     9121 acttgtaggt catccataaa tagatttctt acatcatctt ctatactgtc atactcataa
     9181 aacttaagtt ctgatatatt agacttatat gaataggatg agcaaacact tacttgtatc
     9241 ttggtagcag ttattggttc ttttaatcta accattacat acttattatt atttacaggg
     9301 tctatctttg tttcaaaagg aacatccccc agattgtgta attgaccatt ttcatcccaa
     9361 tatcgtacct caaagtcatt tattgtctta tcagaaacat caaaacgagg tgtagccata
     9421 aaagtatcca tagtataaga tttatcaaaa gttattattg gtcctctatt cgttccctca
     9481 gctgcattat atgttgggaa tgtccaatgt gttgtaaagt ccccatctac tacattgaat
     9541 ttatcaaatg gatttttatc actatattca cttttgtcat atcctgctgg ataagtaaca
     9601 tctacaatgt gatttgttgg tattccaaat ccaccattct ctttaggagt attaattaat
     9661 ctatagttag gtgttgttgg tgcatccatg ctttcagttg tacctatata tgttttagac
     9721 attccactag ttcccaagtg gttagtagct gtcattctaa cttcatatgt tgtattatcc
     9781 ttaagacctt ctatctttga acttgtccca cgaataagct tatcattgta ttttatccaa
     9841 gacccattag agcctttttc tttatagtac aagtcaaaat ctttagcttg tttatgattt
     9901 ttccagctta catcaagaga tttaaattta cctttaacat ttataccttc tggtggttct
     9961 ggttttgtgt ctggaacaac ttgaaattct aatatactat ctgggctaaa ttgtataggt
    10021 ttatagtttg aatctacatt ttctggtact ccgtctaagt tttctggtgt tgcatcttta
    10081 taaccactag accaatctcc atttaaagat tgtatgctaa ctctatatag tccatatgct
    10141 tctatatctc taacttctat tgtattttta ctagtttgta attttttagt agataatact
    10201 tttcccttac tatcaatttt gtctagtata agttcgtatg ctgatacatt tacttcatta
    10261 ttccaataaa aagatacttt ttcatttcca acagcagtgc tagtcaatat tttatttatt
    10321 ttaggtatgt tcatctctgg ctttggtatt tctttatata cattgtttaa aaattcaact
    10381 tttgatattt ctgcaagatt tctatcattt gctgttgtag ctgttatttt tatagtgatc
    10441 ttttttactg ctacttgttt accaagattt attactatat cactattttg aacatctgca
    10501 tatgtttcta atgttgactt actatatgta gatgttgtag agttattatc tttactgata
    10561 ttaccctctt cttttggagt atcttcatct gaattaggtt tatccattga tgaattttct
    10621 ttatcattat ttttatcttg attggtatta gagtttgctt cttgtttatc gctattttcc
    10681 tgttgtttat cattattttc atctttatta tttgagttat tatcagtctg gtttatattt
    10741 tcatctttat tatttacttc ttcttgattt acttcttctt gttcgttatt tacttcctct
    10801 tgttgctcct catcttttaa gtttttcata tattctttgt tttcataagg aatctgttga
    10861 acattaccat ccttgtcagt aacctcaact attccttttg ctggagcagt accttctgga
    10921 gcttttatat tgatgacttc catattaagt gcttctgata aagctatatc cattactgct
    10981 ggtttttcct cagatatttc atcatttttt gaagatattg taactccatt attttctcca
    11041 ctaaataaat tttctacatt accattaata tctacatcac tatttccttt taattcaacc
    11101 ttacttgtat ctactatagg attggtagat actatacttt ctaggctttt tgcatcttca
    11161 ctttgtccta aatttttctg tacataatct aagtcaacaa tatctacctt tccatctcta
    11221 tttaaatcaa attctctttt atttgaatct atattctcta aaaccttatt ataatcactc
    11281 ttatctactt taccgtctcc atttacatca cccattaaga acaattcata atttaaaggt
    11341 ttatttttat tatctacact tgctctattg cttaataaga ctctttgtga ataacttgat
    11401 atatcaatgt ctttaatttc tttgttctta aatccagctc cagagacttc tacattgtat
    11461 tttccaacag gtaaattatt aaatacaata ttataatatg atatattctc atctttattt
    11521 tttatttcac ttctacttgc agataatttt tttatagtat attctatatt atctgtccca
    11581 acatttactg ttcctgaatc tttttctcca agctttatta ttccactatt gttactatca
    11641 tcttttaatc gtaactgcat attagtcata ctttctgact cgctattttt tattggcagt
    11701 gaaaaattta aatctacttc aagactcccc cttaactttt ctgtaactga tttttcaact
    11761 gcccttagct tcccctctaa tataggttct gcaaaaacgt ttagtggtgc actacacata
    11821 gatactgcta gtacacaaga tattgccttt ttcatgaaat aacaatcctc ccttaataaa
    11881 tatctaattt ttcatgaatt taaatactat aagtttcaga caataaaaaa agacactcaa
    11941 agagaatctt aattataaaa taaaaagact ttactctttt tagcaactgg ctgatatggc
    12001 tttaaaagct ttaatcccta tagttttgcg tcattagatt tccctaattt tgccatttaa
    12061 ttttcactac tttcttaccc attttatact atatttaaac tttttaatac aattttttta
    12121 gattaaatta taaaaaaata acatttatat taatttatta atataaatgt tattttccat
    12181 tataatttaa ttatttatat agcttcctta caagcataat tttgtgtata ttttcttatt
    12241 ctttatatct tccataaaat tcatgtgtag catatagatt cttatctcct gttatagcta
    12301 taccaaatcc cattatttca taccctttat gcaacatatt agttccttct tctggatttg
    12361 tcttccactc attaaaaata aagtttccta tatcatctgc atctctattt gtatatacag
    12421 gtttatctgg aattttatat ttaattatat tttcaccttg aactgctata tatccaggtt
    12481 taatctcact ccaatctaag tatttcaaaa aagtagaata tgaatttttt ccatttatcg
    12541 tatgagataa agtcttctta tttgccatta aattagacca atcatttgct agaccctcta
    12601 atctgcttgc tacagataac gctcttactc cattctcttt tctatactgc tttgtaagag
    12661 catatacttt accctttatt ctctctttaa atgctctatc atttggattg taatataacc
    12721 cttgttgatt tggtgaagct tgatttatac attgcttgat aacgttctga tttatgcctc
    12781 ctagtacagt caatttgtct gcaccactta aaatactctt gttactgcca ttttcaacta
    12841 aaactacagg agcatttttt gctattgtag aggcagccaa agcgtctgtt aattgtaagc
    12901 ctttacttag ataaaattct ttactatttt tataaaactc ttgtataact atcttattag
    12961 tttcaaatct atctgtgcca cccaatctaa tagaatttgt gctcttaact aattcgtcgc
    13021 tgattatttc actagaacca agcgcataac tctttaagcc tgtcgtgtca aatcccacac
    13081 tatttccatc tgtaagtatt ataggtactc catctctagc agctactggt gatgcactta
    13141 tcgcatctgc ttctcctgta aatccattgg ctataattac tttatctatt ggcttaattt
    13201 ctttgatttt ttcagctatt tttatacttg tatcaaatct attttcacca aagattcttt
    13261 gtacaacctt ctttttggat agtaactgat tctcaacatt tttacttatt gttgattctg
    13321 atcctattat atacacagtc tttgctttgt ttaatctttc catagttgtg tttggaatct
    13381 ggttttgttt tgtcatcaaa ataggcgcat ttaaggctcc agctaaacca cttgcactta
    13441 gaccatctgc taagctatta tctgagttga ttagtattgc tgtattgtag ttttgcttat
    13501 ctgctatttt tgctgctgtc tcgtatctgt cattcccttt tatttgctca actgatgtca
    13561 acgcatttac cataggagta tttaccatta ctaaaaatag tgtaagtcct atagctaata
    13621 tctttttact attcttcata ttattttcta cccccaatgt acttttcata ttttttatta
    13681 gtactcatac ttatttttaa tcaaaagtta caattttgtt accatatttt atatatgata
    13741 ccatagttat ccatacaact caaacaatat acctaaagtt tttattctct ataaattcaa
    13801 aacattataa tttattttat ttaattaagt tataagttca aaataattta tgttaataag
    13861 atgtttaatc attataagaa gtatcttttt ataataattt ttgcaatatg aatttctcaa
    13921 cattataatc aaaaaataaa ataaggtatc tcaatctaat tctaaatgag acaccttatt
    13981 tttattattt tatcttgtta tttgtatatt ttataatatc tacactatgt cttacatatt
    14041 aacgcattgt ataatcatat attatctaaa taaactttta atattaattc ctatatctta
    14101 ttagttctta gtaaataagt ttattttttc tgctattttt tcaatagcgt ttttagctat
    14161 tccttcacct atttgtacta acttagtagc attcttaagt cttaactcaa ctacttcttc
    14221 ttgtgattta gaaagttctt ctgtagttaa tactattgga gcctttgaac tagctgcaag
    14281 tggtgctgct gtaagtgcat caactaattg tccatttcca cctacataac catcttttgc
    14341 tacatatgct ttagttgcac ctgcatagta tttattgatt actttagcat tagtatcttt
    14401 tctattttct ccagctaatc tttcaacttt attatttaaa tctatatcaa ctaatttgtc
    14461 ttcaacttct tttgatacag aattttctcc acccactatt tctaattctt ttccttttaa
    14521 taatttttca gcatttgcat ctaattcgtt acctgtaact attattggag caccaaattg
    14581 agcagctcta gctgatatac tcatagcatc agcttctcca tttccaccta ctacaaaagc
    14641 agtcttaaat gtatctttta attgttttgc tatttctaaa gaagtttcaa atctatcttc
    14701 tccagaaact ctctcaactt tacttacacc taatttagaa agtttttctt cagtttcttt
    14761 tgatacttta gattctccac ctactatata tattttctta gaagatagat tacttccaag
    14821 tactcttaat atttcagctt ctatttctga tggtagtgaa tcttttttag ataataatat
    14881 tggtgcattt ttttgagagg caagtggtgc tgatgctaat ccatcaacta tagcatcttc
    14941 tccaactaaa attatagctt ctgcttctcc atctttgaat ctctctttac taacttctac
    15001 tgctgttgta aatcttgaat caccagataa aacttctact tttaattctt ttccaccttt
    15061 tacatcagtt aatactttgt gtaagtctct aactaactta tggtttttac ttgaaacact
    15121 tattgtttct ggcttttcat ttgccttgct taaaactata tcaaagcttg atttttctgg
    15181 tttatcttta tcatcactat cttcttgttc ttgagtaagg attgcttcat tttcaaatgt
    15241 taaagcatat ccatttttaa ctttattaac tatttctctt cctaatgtag ttaatctaat
    15301 tccatcaaat aattcttctg ctgatatgtc ttgtaaatct gcgtttttaa ctgttatcgt
    15361 atgtaattct tttgaatcta tgtcactata actcttttca taaccttcaa aagtaccttc
    15421 ttctgatgtt atcacttttg agaaatctaa atgttggtct cctacattta atgtaataac
    15481 atttgctgaa tcttttgctc tttttacttc aactgtattt gtagatttta tagttgctgt
    15541 taacttgtta tcagaatctt ctgctaaagt tgcattgtaa gcattaactg catctactat
    15601 ttcttgagca gttttgtagt tttcaatttt atagtcaact acttttccac ctaacacttg
    15661 atgtccttta tcttgaatag taacttttaa actttgccct tctgttaaag agtttatctt
    15721 attttgtaat tgagttgcat tagttaagtt tacattgtct acttttatat cgtaaactct
    15781 atcatttaca tttgctcctg cttttgtatc ttcaaactta acttctaatg cttttctaac
    15841 ctcttcgatt agtttagcag aatctttcgc acttactgtg taatttttat caacattttc
    15901 tgtcacagta ttgtctgcga atattggtgc tgctgcactc ataactgtag cacctgccat
    15961 tactattgcc atacttctct ttttgttgct taacatattt atgtcctccg aatattattt
    16021 tttatttttt atattcgtat ttcccacaca taaataatat tatatataat attatttttc
    16081 aatatatttt atgtgtttac ataatttact aacacaaaca ctaataatat cttcttgaac
    16141 cccttccccc tgcttagctc tctataatta tgctcaaaga tagaaatcct attttatagt
    16201 gattttctac taaacaatca tatactaaca aaagtattca aactccattt ttatatacta
    16261 ctatgcacta atttttgctt tactcttatt acttaaatat atatcactaa attaatatct
    16321 atctccatat atta
//
LOCUS       DAELJO010000075        16322 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELJO010000075 DAELJO010000000
VERSION     DAELJO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.075"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..1361)
                     /locus_tag="KPB88_002030"
     CDS             complement(<1..1361)
                     /locus_tag="KPB88_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0054308.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLIPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDP"
     gene            complement(1372..2592)
                     /locus_tag="KPB88_002031"
     CDS             complement(1372..2592)
                     /locus_tag="KPB88_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG0054309.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2606..3394)
                     /locus_tag="KPB88_002032"
     CDS             complement(2606..3394)
                     /locus_tag="KPB88_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0054310.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3411..4685)
                     /gene="larA"
                     /locus_tag="KPB88_002033"
     CDS             complement(3411..4685)
                     /gene="larA"
                     /locus_tag="KPB88_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG0054311.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4803..5516)
                     /locus_tag="KPB88_002034"
     CDS             complement(4803..5516)
                     /locus_tag="KPB88_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0054312.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5641..6900
                     /locus_tag="KPB88_002035"
     CDS             5641..6900
                     /locus_tag="KPB88_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG0054313.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7180..7746
                     /locus_tag="KPB88_002036"
     CDS             7180..7746
                     /locus_tag="KPB88_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0054314.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7947..9152)
                     /locus_tag="KPB88_002037"
     CDS             complement(7947..9152)
                     /locus_tag="KPB88_002037"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0054315.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9255..9361)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9453..9821)
                     /locus_tag="KPB88_002038"
     CDS             complement(9453..9821)
                     /locus_tag="KPB88_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG0054316.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9873..11150)
                     /gene="obgE"
                     /locus_tag="KPB88_002039"
     CDS             complement(9873..11150)
                     /gene="obgE"
                     /locus_tag="KPB88_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG0054317.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11261..11551)
                     /gene="rpmA"
                     /locus_tag="KPB88_002040"
     CDS             complement(11261..11551)
                     /gene="rpmA"
                     /locus_tag="KPB88_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG0054318.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11565..11882)
                     /locus_tag="KPB88_002041"
     CDS             complement(11565..11882)
                     /locus_tag="KPB88_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG0054319.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKGTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11897..12208)
                     /gene="rplU"
                     /locus_tag="KPB88_002042"
     CDS             complement(11897..12208)
                     /gene="rplU"
                     /locus_tag="KPB88_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG0054320.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12309..13730)
                     /locus_tag="KPB88_002043"
     CDS             complement(12309..13730)
                     /locus_tag="KPB88_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG0054321.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13748..14449)
                     /locus_tag="KPB88_002044"
     CDS             complement(13748..14449)
                     /locus_tag="KPB88_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG0054322.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14451..16295)
                     /locus_tag="KPB88_002045"
     CDS             complement(14451..16295)
                     /locus_tag="KPB88_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG0054323.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5021 a         3082 c         1744 g         6475 t
ORIGIN      
        1 ttagggtcaa aagctagctt tatattttta attaacatca tataagcatc tgaatttgat
       61 tcatgtaagt actctttttt agcatatcct atgccatgtt caccagaaac ttgaccacta
      121 agttctctag attttttata catatagtca aaagtttctt ttaatcttat tttccatgta
      181 ttatcatcca taccatcttt taaaatatat atgtgtagat ttccatctcc tgcatgacca
      241 aaacttttta ttcttatttt taatttgtct tgaagctcgt gagtgtatct tataaactct
      301 gctatcttat ctcttggaac aacgacatca cattcatcca tttgtgtcgt agatgccttt
      361 atagcttcta aaaatgcacc tctggctgac catatagaat cgtttctttc ttgtgtatct
      421 gatataaata catctaatgc accattttct aaacataaat tagctacttt ttcatattct
      481 ttttctatat cttcagtgct atttccatca aatgtaagta aaagataagc atctgatgta
      541 ttatctggaa actttttacc taaaaattct tctgctgcca atattacatc tctttccata
      601 aattctatag ccgttggtat tgattttgat tttattattt taggaacagt ttctattgcc
      661 attgataaat ctggaaatgg tataagcaaa cttatacttt tctttggtaa aggaatcaat
      721 tttaatatag ctttagtaac tattcctaaa gtaccttctg agcccactaa taaatccttt
      781 atactatatc cagaactatt tttaactacc tttccaccta catttataat ttctccattt
      841 ggtagtacaa cttcaagacc tcttacataa tcccttgtaa cgccatattt tactgctctc
      901 ataccacctg cattagtgtt tatattacct gcaattgtag cacttttttc acctgggtca
      961 ggtgggtaaa ataaatctct atcttgaaca tattgaccta ttgtcatcaa taaaacacct
     1021 ggttctactg tcaatgttaa attttcataa tcaacttcaa gtattttatt cattttacat
     1081 agattaatca ttattccacc atttatggca acagcagcac ctacaagacc agtaccttga
     1141 cctcttggtg tgactggtat attattttta tatgcgtatt tcattatttt agatacttgt
     1201 tcagtttcaa gtacatttac taaaacttca gggtacttct caatccctcc aagttcatca
     1261 tgtgaaaaat cttcatttat atcacttcca actaatacat tttcttctcc acaagtatct
     1321 attaaaaaat ctatatcttt tttatcgatt aatttataca tttctatccc cctatttagc
     1381 tgctgatgcc atatattttt gattattatc tttgttattt tctatttttt caatcaattt
     1441 aggaataata tcataaatat cacctattaa agctaaatgt gctacatcaa gtataggcgc
     1501 tttatcatct ttatttattg ctactatata gtcagcaccc tgcatccctg caacaaattg
     1561 aactgctcca gatacaccac aagttattat caatttaggt tttacagttc taccactaag
     1621 acctatttgt tttttagcat ctatccaacc tgcttctatt acaggtcttg ttcctgccac
     1681 ttgagcgttt agcttatcag ctaatttata tatcatctcc atatcttctt gtttttttat
     1741 tgctctactt gccacaacta tcacttcagc ttcttccaga ccaacttctt tatttttttc
     1801 ttttacactc agtaccttaa ttttagattt tagttctttt ttttctactt tacataaagt
     1861 tatctttcct gtagtattct ctattttttc tggtgctgaa aatattttat atcttacagt
     1921 ggcaaattgt ggtctattat taggtgtgtt tatatgagcc attatatttc caccaaatgc
     1981 tggtcttatt tggtctaaat cagtatttga ttgaatatca agtattgtac aatctgctgt
     2041 aagacctgtt ctaaatctag cggcaagtct tggagctaat gacctaccta atgtagttcc
     2101 accaactaat actatcgttg gttttatttt atctataaag ttttctattg cttttgaata
     2161 tggttctatc ctaaaatctt taaattcatc ttcatcatat acaaatactt catccacccc
     2221 ataagaaatc aaattactgc acttatcttt tatgtctttt cctacaaata cacaatacac
     2281 aggttgtttt atttttgatg ccatctcacg agctttacct ataagctcgt acgttacagg
     2341 atgtatattt ccattatgat gttcaacata tactgcaatt cccctccatt catctttatt
     2401 aatttgtact ttactactct ctataaattc aaatgcgcct tttggtccac ttttgacaca
     2461 caacttgcac atcttacagc cagcacttat ttctactata cctgactttt cttctatagc
     2521 cccaaaagga catatttcta aaattctctt taagtcagtt attttatctt gatttacaac
     2581 tatctttgac atttattact cccccttaca ccaactttaa ttcctctaat ttatttacaa
     2641 gtgcaaaact tagttcatct gcatttcctc ttacaatatt tttatctgta tttggctttg
     2701 gaggaaatat tcttacaact tgagtaggag aaccattaag tccatagtta ttttcatctt
     2761 tatcttcaaa gtcatctaag ctatatacag gtatttctct atctttagta ttaagtttta
     2821 atttaaatga aggtagtctt ggttgaaaaa tgtctttttc tacagttatt aaacatggat
     2881 attcaatctc acatacttgt aaatcatttg gcatatctat ttcaacaact atactttcat
     2941 ctttaacttc aattaatttt gtaacgttag taacatgagg tatatctaaa aattcagcta
     3001 cttcaggtcc cacctgtgct gtatcaccat cagtagtttg ttttccacaa attattaggt
     3061 ctacctttcc catcttctta ataccttgtg atatggtata ggaagtagcc aatacatctg
     3121 ctcctccaaa tctcctatca cttattaatg ctccttcatc agctcccatc ataaaacttt
     3181 cttcaattac cttctttgcc tgaggaggac ccatgctaag tacttttaga ttcgctttct
     3241 tatcttcctt tattctaaga gctgtttcta aagcatatag gtcataggga ttcatttttg
     3301 aatctactcc atctcttttt agtactcctg tagtttcatc aacttctact gaagtcgttc
     3361 caggaacttg tttaatacat gtcaaaattt ccataaagta cactcctttt ttatttaact
     3421 ataactgata ctccatcagg tataactact actttgctct ccttaccaac tatactattc
     3481 gccatttcta aagcttcttt aaagttaaat gcatgtttca tatgcatttt ttttatcatt
     3541 tcaggattac acttatctgt taccattatc acagtatatt tagataatat tctcgctagt
     3601 atctgaaatt cccattggtc tggaatcgta tctactctat cgacacttct aattttatct
     3661 aaaagttgtt ttggagaatc tgcatttgct acattttcat aaaaagattg acctccatga
     3721 ccatcattac atgctgaaat cattattatt actccaccgt ctttacaagt agcttcagct
     3781 gctgtcattc ctttaacaga ttgataaata ttttggtcta gtggatatcc accatttgta
     3841 gttacaacaa tatcagattt tatagaattt acttttgata actctgttac aaatctacat
     3901 cctttagtat gtgctttttc gacatcacca gcaaaggaag caattatttt tttatcttta
     3961 tctattacaa cattcaatat gaatgctaat tttgcaactt tagctgcata taccatatcc
     4021 tcatgaatag gattattatt caattttcca gttcttgaat tatcactatc tataaagtca
     4081 ctacaatgat tgtacattat agtctttgca gatgcgattc ctggaagtac actttttctg
     4141 ccacctgaaa aacctgcaaa gaaatgaggt tctataaaac cttctgctat caaaagttct
     4201 gcctcatatg ctagtttgtt tatatataaa tctccacctg aaggcaatat ccctacttta
     4261 cacatagatt tatcatcagt agatatatgc ataactatat cttcattatc tactatttct
     4321 tctcccattt tataaattag ctcttccctt gtacttggcc tatgaaatcc agttgccact
     4381 ataatcttca catcaatttc tggattaaca ttcctaatcc ttcttaatat aattggcata
     4441 gttattctac ttggtacagg tcttgtatgg tcacttgtaa ttatgactac attctttttc
     4501 cccttaacca gttcttccag agaattagag cctataggat tattcataga ttcttcaact
     4561 atctcttctt gagtcttatg ttctatataa ttatccgatt ttgactccaa aacaccgaga
     4621 aaacgttcat caggtatttc taaaatcatt ccttgttttg aatacggtac ttttaacttt
     4681 accattttta atctcccatt ataataaact ataaaattgg tatgtggtca taccagtttt
     4741 acatttatat gatagtacca ttatctacca tttgcaagaa taatttattt tataacaaac
     4801 ttttaatttt cattttttct ataattttta cttataaaat ccatatgttc ctttgctttc
     4861 tcacatgcaa gttctacatc attttctaca actgcattgt atatagcttc atgctgttta
     4921 agcaatatat tttcattaaa aggttctgcg attatttttt ctcttgcatc ttctataaat
     4981 ccatcaaata attttgatgc tgtcataaaa agactttgaa ttaaataatt tttacttaat
     5041 gttgctagtt tactgtgaaa tttttggtct aataacacca gtactcgatt attatctttt
     5101 ttattagttt ctaccctcat ttcatctatt atatgtttca attctataac ttcagaagaa
     5161 gttattctct ttgaagcaag ttttgcaatc tcaatttcta ggattattct aagctctaat
     5221 atatcttcaa gtgtaccatt attaagttta aaaatcatag ataaaggttc tattaaagtt
     5281 ttttctatat ttgtacatat aaaatttcct tcaccctgtc tgctttctat cactcccata
     5341 gtctctaata ccctaatagc ttcccttatg gaagttctac tcacattcat ttgttcagac
     5401 agttctcttt ctgatagtaa tttatccccc ttttttaact ctccatttag tattttatat
     5461 tgtatttgct caattacttg ttcataaact tttttactag ctatactatt aaacattttt
     5521 tcctccaata tgataatcgt tgttgtaata tatatctatt atatatctta agccattaca
     5581 ttcatatatt tttattactt ttaatataat atcttataac tcaaatttac aggtgatatt
     5641 atgaaaaaaa ataaaattat tgcttttttt gctccatcta tcatagttgg agttctagtt
     5701 ttagggatta tcctataccc aaatgaagct ataaaatctg caagagaagg cttttctata
     5761 tggtctaatg ttttagttcc atcactacta ccatttataa ttggagcaaa cttaattgtt
     5821 gatttaaaaa tagtggatat tatagggttt atcatcaatc ccataactag atttgtattt
     5881 aatgtttcag gaaagagcgc tttagctttt gctatatcta cagtatctgg ttatccagta
     5941 ggagtaagtt tagcttctga atttaggagt aatggacaaa tttctaagta tgaagcacaa
     6001 agacttgtgt ctttttgttc tacatcagga cctttattca taataggttc ggttggtaca
     6061 ggtatgttcc aaaattcaaa cttaggttat ttaatgattc tctgccatta tctaggaact
     6121 atattggttg gtcttttctt tagaaattat ggtaatgaaa atcttccaaa gacaaaattg
     6181 gatttaaaaa caaatataaa aaatgtaatc aacattagaa attcaagtgg gaatggtttt
     6241 tttgttttat ttggaaatgc tgtatttaat ggagtaaata cattacttac agttggtgga
     6301 tttgtaattg ttttttcagt agtatttaaa atactatctc tttttaatgt aatctcatta
     6361 atagcatcag ttatatattt gcctctttca tttttaggtg ttagtaaaga actctgtcaa
     6421 gcatttgtaa gtggtctatt tgaaattact ataggatgca ataaagtatc tagtataaca
     6481 agttctccag aaattttaag agcatcttta gctagttttt tgataggatt tagtggatta
     6541 tccatcctag ctcaatgttg tacatttcta tcaaaaacgg acatagattt aaaattatac
     6601 atactaagta aatttgcgca tggagtttta gctgctatct ttatatttat actttatcca
     6661 attgcaaact ctgctgtgtt ggtttctagc tttgctgata catacaacgt tatatataat
     6721 aatttaatct ggttttatta tcttagctat tatgatacaa ttcttcaaat tgtagtaata
     6781 atttatctaa tctcagccgt atttattgca aaaaaacata tgaaaacttt atctacaaat
     6841 gggaaactaa tgcgatataa aaatactttt ttcactaaga aattttcacg aaaaaaataa
     6901 aactaaaaat aaaactaata aaacttaact aaatacttcc atttttttat tatatttgtc
     6961 ataattttaa taaccttttt aattagttta acagcatagt tatttcatca caatttaata
     7021 attttattag aaatattttt caaaaaattc tccttaatca catatatcac acttgtataa
     7081 gtttactggg aaatttttcc attatataat atttcattta tattatataa taaataccga
     7141 cctaaactta ccaaatcaaa ctaagaggag tggttgaaaa tggcaacaag acctatagaa
     7201 atagacgaat ataaaaagat aatggaacta ttacatactg gctttactta tagtgaaaat
     7261 ggcgttgaaa aaagatttag aaaaaatcct aaagttgcat tggcactaat gttagaagct
     7321 aatttaggtc taagaatatc agatatttta agattaaaga taggtaattt taaaggtaat
     7381 atgttggaaa taaatgaaaa aaagacgggc aaattacagt atagaccagt taataaaaat
     7441 ataattgagt ccataaatga acatgcaaga aagtataatc ttaaatcaaa tgactactta
     7501 gtaaatataa agacaaaggc tattcaaaaa cagttgagga taatttgtaa atacttaaat
     7561 ctgtataata taagtacaca tagctttaga aagctctatg ctactactca atttgaaaag
     7621 agtaacaata atttagagtt ggttaaagaa cttttaaatc acagctctgt agccactaca
     7681 cagcgatata taagggtaac tcagcaagct attaatgaag ctagtgaaaa tttctttatt
     7741 ggttaattat aacgtataca tttttctatt atcttattaa ttcaccagat attaaaaaag
     7801 agtgtctcaa aatgaacttt ttagttcatg agacactctt tttttataaa atcaaatata
     7861 tttttattat ttcaacactg gaaaaaaggc ttatctctct attttgagac accctattaa
     7921 attttcataa ttcttgcata aacatattaa tctatcttca ataatttttt agctgattca
     7981 ctgtaattat ttctttttac aatttccaaa actctatcaa tctcatgttc tgactttgca
     8041 cttacaaaaa tcgccaattc ttcatcatct ataatatcat aactatctac atatttatct
     8101 aaacattcta aaattccatc tttattatta tacccaccaa atctaactac tgcactacac
     8161 tcatcttcta taagtccact taaatccaat ttcccttttg aaaaagactt tatatttgaa
     8221 cttctattat ctattatatc tataatatct gcataaacag cacttcctgt tgcacctctt
     8281 ccagctccct tacctacaaa agaaagttca cctactgaat cacctactaa aataactacg
     8341 ttgaactcat tatctatctt tgaaagcata ttattattgt ccactaatgc tggtctaaca
     8401 aatccactta cttctcctga ttcatactta cttctagcaa ccaattttat cttacaattt
     8461 aatttttttg catattctat atctttttca tctattgaag ttattccttc taataataaa
     8521 tctttccaat atactatttt tccataagat aaagtagaca atatagataa ctttctagca
     8581 ctatcatatc ctaaaacatc tgcatctgga ttaacttctg caaatcccaa ctcttgtgcc
     8641 tgttttaaag cgacatcata cggtaaattt tcatcataca tctttgataa tataaaatta
     8701 caagtcccat ttaaaatagc gtttatgctc tttatattgt tgccttccag tgattctatc
     8761 aatggtttta aaactggaat accacctgcc acagatgctt caaacttgat gctaacatta
     8821 tttaagttag ctaattctat aaggtcacta cctttttcag ccagtaaatc tttattagct
     8881 gtcactacat gtattttttt atttaacgct cttgttacat aatcaaaagc tggatttaat
     8941 cctccaatta cttcaactag tatatcaata tccttgtcaa atatatcttc tatatcagta
     9001 gttatcttat caaaatactt cttatacttg tgtttttctt tatttttaac taaaattcca
     9061 acaacttcta tatctctttt tgaagtatta ttttctatta aatcaattaa tccacttcct
     9121 actgttccat accctaatat tgctatcttc actgtaacat acccccatca ttttgattat
     9181 attaaattat ttatatatat aaatatacca ataaaaaaag cttgagtata aactcaagcc
     9241 atatgacggg tttatactca tctttcagca taaatgctgc aggatttagc accaatgtat
     9301 aattaaatat actggttgcc gggcttcaca gggccagtcc ctctgccact cttgataaga
     9361 ttgctaattt aaatttatgt ttaatagtat aatacaattt cttccaattg tcactatttt
     9421 tttaacttat ttccctaaaa ggatataaac tattatctaa cagctctttt agttggttta
     9481 cccttttttg taacattatt atttttagtc tttgctttcg cctgttcatg tcctggaaaa
     9541 agtattttag gctcttcaat attcctcttg taaatagtaa atttaaaacc aatagcctgc
     9601 acaaattcag ctcttaaagc ttcacatact gcattagcag tttctttagt atcaagacct
     9661 gcattttcta atatagtaac ttttactaat tctctagttc ttaacatacc atctaattgc
     9721 tctaagaaac tctctgtaac accttcttta ccaatttgtg tagtaggctt taaagtattt
     9781 gccaaagatc ttaaatacgc tctttgtttt ccttttaaca tattctcacc tctataattc
     9841 tatacttaaa ttatctataa tctatattta aattaattgt aaaactcaaa ttctaattca
     9901 taaattttaa ccacatctcc atcttctatt cccatttctc taagcctatc aaaaacacct
     9961 tgactctcca tagctttttg gaaatattga agggattcca tatcatcaaa atttacagaa
    10021 tacattattc ttctaagtgc tttacctgta actacataca caccatcttc aatatctata
    10081 cttaactctt cttcagtacc aatatcaagt tctggtctat acatttcttc ttctgaaact
    10141 agctccacat cttctacttc ttttagcatt ttagaaacat aggctattac ttcatctatg
    10201 ccttgacgag ttgctgctga cattttaaat actgtatatc ctctgccttc taattcactc
    10261 ttaaacttct caaacttact ctcatcttct aatatatcaa atttatttgc aactacaact
    10321 tgtggtctct ttgaaagctt ttcattgtac aatttaagct catcatttat cttgtcaaaa
    10381 tcttcaatag ggtctcttcc ctctaatcca gatatatcta caatatgtat caataccttt
    10441 gttctctcaa catgtcttaa gaactcatga cctaatccta tgccttcaga agcaccctct
    10501 ataattccag gtatatctgc aagtacaaaa ctatctccaa acttagtttg aacaacacct
    10561 agatttggtg taagtgttgt aaaatgatag tttgcaattt tgggtttagc tttagttaca
    10621 actgatagaa atgttgattt acctacattt ggaaatccta aaagaccaac atctgctatc
    10681 attttaagtt caagagttat ccatctttct tctccatctg taccagattt tgcaaaagct
    10741 ggagcttgtc ttactgcatt tgcaaagtgt tggttacctt taccacctat accacctttt
    10801 gcaactatag ctgtatctcc ttcttttttt aaatctgcta atacaagacc tgtagcctca
    10861 tctcttatta ctgtacctgc tggtactttt aatactaaat tttctccatt tttaccagct
    10921 ctttttcctt tgcttccgtc gccaccattt tgagcttgat attttttctg gtacttaaag
    10981 tccattagag ttctaagccc caaatcaact tcaaatatga tgcttgctcc acgaccacca
    11041 tctccaccgt ctggaccacc agctggaacg tatttttctc ttctaaatgc taccgaacca
    11101 tttccaccat ttccagcctt aacaaatatt cttgccttat ctataaacaa attcttcacc
    11161 tacttaatta aaaactttta attagcatta ccaaaaatac cattataaat tcatcagtat
    11221 tataagaaaa aggagtgcct caacactcct tttatgtata ttactcagct attgctactg
    11281 gatatatact aacttttttt ctcttcttgt cttttctttc gaatttaaca gtaccatcaa
    11341 ctaatgcaaa taaagtatca tcactacctt ttccaacatt agttcctgga tgtattttag
    11401 ttcctctttg tctaactatt atacttccag cagttactaa ttgaccatca aatctcttaa
    11461 cacctaatct tttagatata gaatctctac catttttaga agaacctacc ccttttttag
    11521 atgctaataa ttgtaaattc atgtttaaca tatggagtca ccccctactt ttttaaaatt
    11581 tttatatttt ttgggtattc ccgtttaatt tctttaaccg ctaattgaaa gtgatttagt
    11641 aataattgtg cgtcttttac atgcgtacct ttgactatac actctatatg accttcttct
    11701 cctactactt tttcaaattc aatcttttgt aatttgtcta aagaatttat aactgcaata
    11761 gcattagatg taactgcagc acatactata tcatagccta tttcagcaaa atcagcatgt
    11821 ccctttaaac aaaaaccttg caacaagaaa tcatcattat aataatattt aacttttatc
    11881 ataacttaac caataactaa gcgtttattt tttctacaac tatcttagtg tatgattgac
    11941 gatgtccttg ttttcttctg taatcttttt ttgctttata cttgaaaact ataactttct
    12001 tagctttacc ttgttcaaca actttagctt gaacagatgc tccttctact actggagaac
    12061 ctaattttaa ttctccatct ttagagcaag ctaatacttc gtttaaagta acaacatcac
    12121 ctgcgttagc ttctaatttt tcaacgaata atacatcacc ttcagaaact ttatattgct
    12181 ttccaccagt ctttactata gcgtacatta aatacacctc ctcggcctca aactcgccag
    12241 tacaaggtac ttcatttgaa gatttaaacc tttactgtgc ggcattacaa ttattaatta
    12301 taaaatattt acattttatt gtcaactatt ttatcgaaaa taacattggt tttattgtgc
    12361 tttaatgtac tatttgcgtt taaagaaatc tttatattat atttttcacc aattttatca
    12421 attatatcca tataatattc ttttatttgt tcaaaaataa aatcattaaa ttctaccatt
    12481 atgtttttgt aaacagtatg ttcacttatt ctcataattt ctttttcaat actatctagt
    12541 atataatgta cagattttat tgtttgttca ccatcacaac atggacaaga cattaaataa
    12601 tacttatcaa tggattcttt ttctctacgc ctagcaactt ctactaaacc aagttttgtc
    12661 atacctaata cttcagcttt tcttttatct ttattaaatt cttcttctag gatgtgtaaa
    12721 atctcctttt gatgcttctt tttatgcata tcaataaaat cgattattat tatcccagct
    12781 atgtctctaa ttctaagctg tcttacaatt tcttttgcag cttcaagatt agttttataa
    12841 acggtttcat caagtttacc agttccaata aacttacccg tatttacatc tataaccgta
    12901 agagcttctg ttttttcaat tattaaataa cctccactct ttaaccatac ttttttattt
    12961 aataattttt ctattttaga ttctattctg tataaatcaa atacatctct attttcctca
    13021 agacacagtt tatcaacata agtcttatct atacctctta aaatagactt aagttcagag
    13081 tacttatcat aactattggt aattatagat tcaatatcat cattcacatt atcttttaca
    13141 tatttagttg caaaatctaa agacttatat aataatttcg gacctatacc aagtttatat
    13201 tcttttaaaa tgttttcata ctttaatttc aactcttcta tatctttctt tatttcatca
    13261 tgagtgcaac cttgagcctc agtccttatt attaatccta gattttcctt atttactgcc
    13321 ttagtaattt tttttaatct aaatcgttct ttttcatcag taattttatt tgatattgta
    13381 gttctatcat tggaaggtat atagacaata taccttccag aaatactaat ctcagtgttt
    13441 aattttgcgc ctttagttcc tatttcttct ttatttatct gaaccaaaat atcctgacca
    13501 gattttatag tacttccctt ttttaattgt aaataagcaa gtttcttaaa tccaatatct
    13561 acaaaacaag cttctattcc tggtataact ttttttacga ttccacgata tatattggat
    13621 acagtcttct tatttaaatt gtcttcaaca aaaagttctg ttaatctttc atcttcaagt
    13681 actgctgttt tctgggaccc tattagggac tcaataacta tctttttcac aacttcacct
    13741 ctcgagctta cataggagtc actaattccc catcttctaa tacatataaa tctcttctca
    13801 atatatcaac atctaatggg tcaatatcaa gttcaaacat ctctaatatc tttggtataa
    13861 gtatattagt atttaaatta gtttttgagc ctgtagctat agtagcagta agagtaatat
    13921 gttcatcttc taaatttaaa acatcaaagg ttcttatcat aggtcttata tctacttcaa
    13981 ccatcttacc ttttttattc ttttttgtta tgattatttc tttattattc ataaagtcaa
    14041 ttacttttct ttttacaaac tctttcgtca taggtctttt caaatctata ttaaacaaat
    14101 attctccata gtctatcaca gaagaaagag aaggtgtttg tctatcaatt tcttttgctt
    14161 tgataaaatc tatcccatct ggaagttgaa ttgatacttt atttaaaaat tcctctactg
    14221 ataaatcatc ttcttctatt tcaatatcta catactcacc ttgactttct gtccctaaag
    14281 ccaaagcatt gccataactc attttagggt gaggattaaa tccttgagaa tgtgataagt
    14341 ttatttcagc tcttctaaac gctctttgta acaatctctg aaggtcaaga tgtgatatat
    14401 atatcatgtc gccttccttt ttaaatttta ctcttattat cttactcata ttagcaaagc
    14461 ccccttccta tatcatgtat atttattcca caaccattgc agttatgtct acaatcagaa
    14521 gtgatttttt cttttttagc attttcgtct tctctaatta agaatttttt actaatacct
    14581 acatctatat ggtcccaagg aaatacctct tcatagtttc tatttctatg tgcataaaaa
    14641 tctatagaaa gattattctt ttccatagct tctttccata tatctaaatt aaaatgttct
    14701 gcccatccat caaatttagc acctagtcta aatgcatcat atataacttt tcctattctt
    14761 ctatcgcctc tagctactac tgcttccatc aaactagtct ttgaatcatg ataattgtat
    14821 tttatattgt tatttcttaa tttatttact aaatgtctct gcttattaat aacttcttct
    14881 gtagtatctt gaccatgcca ttggaaaggt gtaaatggct ttggtacaaa tgtagaagta
    14941 ctcactgtaa cactaaaact tctctttagc tttcctccat ttacttttct ataaatatca
    15001 ataactttat aagctaattt agctatacca tctaaatctt cataagtctc agttggtaaa
    15061 ccaatcataa aatacaactt aacactattc caccccatct caaaagctct ttctgttgca
    15121 ttttccaaat cttcttctgt aacaccttta tttataacat ctctaagtct ttgagttcct
    15181 gcttctggag caaaagtaag tccagatttt cttacttgtt gtattttatc agcaatttcc
    15241 attgaaaaat tatctaatct aagggatgga agcgaaatcc caatattatt agatgcatat
    15301 tcatctacca aataatcagt caactctgat aacttactat aatcactcgt actaagtgat
    15361 gataatgata tttcatcata tccagtattc ttaactaatt tatctaatat ttctttaagt
    15421 cttttaacac tcttttctct tattggtctg tatatcatac ccgcttgaca gaatctacat
    15481 cctctagtac atcctctaaa taattctagt actattctat catgaacagt atctatataa
    15541 ggtactatta gtttttctgg ataatctaca gtctcaacat cttttattat ccttttagta
    15601 ggatttttag taatcccttc tctattagga gttacacttt gaactgtacc atcttcattg
    15661 tacttgacat cataaaaact tggtatgtat actccttcta tgctagatat tttatataaa
    15721 aaatcttctc tagtcgtttt atttttcttc cattctttat actcatttac tacttctaaa
    15781 ttaacctctt caccttcacc taatattact acatctacaa aatctgctaa tggttcggaa
    15841 ttataagcac aaggaccacc aaccattata aatgggtctt ccaaagttct ttcttctttt
    15901 aatattggta tattggctaa atctaatata tttaagatat tagtgtatga aagttcatat
    15961 tgaagtgtaa aagtgacaaa atcaaagttt gttataggct ctcttgactc taatgcaaat
    16021 aatggtatac ttttttctct cattatattt tccatatcaa ctgcaggaga atacactctc
    16081 tcacaaaata catcttcatc cttatttatt acatcatata atatatgact ccctaagtga
    16141 ctcataccaa cttcatataa atctggaaag caatgtgcat atcttatcaa ttcactatta
    16201 ctagtatctt tatgaattga atttatttca tttccaagat atctagcagg cttttctact
    16261 ttttttaaga tttttttcaa gtctacttta ttcatttatt aaaattcctc caaacaagct
    16321 ct
//
LOCUS       DAELJO010000076        16291 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELJO010000076 DAELJO010000000
VERSION     DAELJO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.076"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..325
                     /locus_tag="KPB88_002046"
     CDS             <1..325
                     /locus_tag="KPB88_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBG0054324.1"
                     /translation="LIVLSLKISQGTLLKKYNDKYVKILDIVNISKNNSIIILKIGEE
                     GCIMASSSSSMNKIKDLTKEEILKIENDIKERNFKIQYFKMERIKKKYDFSRYIGKFT
                     KKEDK"
     gene            326..991
                     /gene="fliP"
                     /locus_tag="KPB88_002047"
     CDS             326..991
                     /gene="fliP"
                     /locus_tag="KPB88_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG0054325.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            1004..1273
                     /gene="fliQ"
                     /locus_tag="KPB88_002048"
     CDS             1004..1273
                     /gene="fliQ"
                     /locus_tag="KPB88_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG0054326.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            1285..3099
                     /locus_tag="KPB88_002049"
     CDS             1285..3099
                     /locus_tag="KPB88_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG0054327.1"
                     /translation="MIFVFIRIVAFFGTLRILFSAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVITGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNEIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            3108..5183
                     /gene="flhA"
                     /locus_tag="KPB88_002050"
     CDS             3108..5183
                     /gene="flhA"
                     /locus_tag="KPB88_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG0054328.1"
                     /translation="MNKFNLKENMDAIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVELIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIIVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            5158..6174
                     /gene="flhF"
                     /locus_tag="KPB88_002051"
     CDS             5158..6174
                     /gene="flhF"
                     /locus_tag="KPB88_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG0054329.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLELN
                     HEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQTINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            6171..7049
                     /locus_tag="KPB88_002052"
     CDS             6171..7049
                     /locus_tag="KPB88_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG0054330.1"
                     /translation="MIDQAQILRKMAMEKRGLDEIIVENENTPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            7063..7764
                     /locus_tag="KPB88_002053"
     CDS             7063..7764
                     /locus_tag="KPB88_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG0054331.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            7780..8202
                     /locus_tag="KPB88_002054"
     CDS             7780..8202
                     /locus_tag="KPB88_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0054332.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENCNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            8187..8372
                     /locus_tag="KPB88_002055"
     CDS             8187..8372
                     /locus_tag="KPB88_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0054333.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            8413..9186
                     /gene="flgG"
                     /locus_tag="KPB88_002056"
     CDS             8413..9186
                     /gene="flgG"
                     /locus_tag="KPB88_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0054334.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            9207..9968
                     /gene="flgG"
                     /locus_tag="KPB88_002057"
     CDS             9207..9968
                     /gene="flgG"
                     /locus_tag="KPB88_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0054335.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            9991..10872
                     /locus_tag="KPB88_002058"
     CDS             9991..10872
                     /locus_tag="KPB88_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0054336.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            10875..11249
                     /locus_tag="KPB88_002059"
     CDS             10875..11249
                     /locus_tag="KPB88_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0054337.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            11281..12684
                     /locus_tag="KPB88_002060"
     CDS             11281..12684
                     /locus_tag="KPB88_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0054338.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKENDVKSKKNTVKN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKPSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            12800..14737
                     /gene="htpG"
                     /locus_tag="KPB88_002061"
     CDS             12800..14737
                     /gene="htpG"
                     /locus_tag="KPB88_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG0054339.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLENCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            15124..16254
                     /locus_tag="KPB88_002062"
     CDS             15124..16254
                     /locus_tag="KPB88_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0054340.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPIADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRTFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT         6669 a         1721 c         2906 g         4995 t
ORIGIN      
        1 attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat ataatgataa
       61 atatgtaaaa atactagata ttgtaaatat aagtaaaaac aatagcataa taattttaaa
      121 aataggtgaa gaaggatgca ttatggcatc ttcttcctct agcatgaata aaataaaaga
      181 tttgaccaaa gaagaaatat taaaaataga aaatgacatt aaagaaagaa attttaaaat
      241 tcaatatttt aaaatggaaa gaatcaaaaa gaaatatgat ttcagtagat atattggaaa
      301 atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt tggctgatgt
      361 accgtataca agttcaatgc aattgttttt atttctaact gcattaatgt ttttaccatt
      421 tgtaatgttc atgatgacaa gctttgtcag aatagttata agtttatcgt ttttgaaatc
      481 agcattagga gcacagcaag caattccaag ccaaattttg gtagggcttg caatagtatt
      541 gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat tacaaccata
      601 tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagaaggac ctattaaaga
      661 gtttttatta aagcaaacca gacagaccga tttagattta tttgtagaac aggctggttt
      721 aaaagaaaag aaattaaccc gtgaaaatat accactatct gttgtagttc cagcatttgc
      781 tataagtgaa ctaaaaactg cttttcaaat aggtttttta atatatatac catttttgat
      841 aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt tacctccagt
      901 catgatatca ttaccattta agttattatt atttgtaatg gtggatggat ggaacttgat
      961 agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg aatttatgat
     1021 aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc cagtactcat
     1081 tgtttctctt gtagttgggc ttttaataag tgtatttcaa gccacaactc aaatccaaga
     1141 gcaaactttg acttttgtac caaagctaat agcaattctt ttagtagtag ctattggtgg
     1201 aagctggatg ttacatactc ttattagctt tacttcggag atattcaata tgatgtctct
     1261 tataactaaa taagtaggat atatatgatt tttgtattta ttagaatagt agcattcttt
     1321 ggaacactaa gaatactatt ttcagctggt acacctgctt tatttaagtc actctttgca
     1381 atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac aaatatagac
     1441 aacattgtat tgtttacctt atatggtata aatgagacca taactgggat tatgcttgga
     1501 tatataacca acctatgttt ttatagtatc agaatggcgg gttctatgat ggaccaacag
     1561 atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac aactttaatc
     1621 aataatctta tgaactggac agcacttatg atatttttta gcatggatgg tcatcatgtt
     1681 ttaattagag gcataaggta tagttttgag ttaattccaa taggaaaatc attcgtagat
     1741 aataatatag actatataat aaacatattt gtgcagtgct ttttaactgg attcaaaata
     1801 gcaataccta ttgtactttg tctgcttatg gcagatttta tattagggct tatatcaaga
     1861 agtgtacctc aactaaatgt aatgataatt ggtatgccat taaaaatact ggttggtatt
     1921 gcattattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt attaagtcaa
     1981 ataccaaaga tgtatgaggg aacttttgca ctagctccta tgttttttat gggttcaaca
     2041 gataaaacag aagaagctac tccaaagaaa aagggagaac aaagaaaaaa aggtaatata
     2101 gcaaagagta aagaattacc cgttgcaatg actctacttg cattcacttt attagtacct
     2161 acattatttt catatgtagt ggatacactt aaatcttcgc ttaattattt tttgagtcta
     2221 gatttttata tgaacataaa ttattcaagt atagagaaat tagttataac aggattaatg
     2281 gattttttta agatattttt accaatagct attcccttct tagttttagg tatagtagca
     2341 aacctatttc aagtgggaat actgtttact ggtgaaactt taaaaccaaa tttatcaaaa
     2401 ttaaatccaa ttagtggatt caaaaacatg ttttctatga gaagtttgtc aaccttaata
     2461 aaggatacag cgattatatc tattttggct tacataggat atacattctt ccaagataat
     2521 tatttagata tattaaagct aggtaatata tacctaccaa ctttaatgta cacagttaaa
     2581 gacttagtat attcaatatt gagtaaaatt tgtgtagcta tgatagttat agcagttgca
     2641 gattatgttt atcaaagata cagtcataaa aagcaactta gaatgacaaa acaagaagtt
     2701 aaagatgaat ataaaaactc agaaggagac ccagaaataa aagctaagat aaaacaaaaa
     2761 caaagacaaa tttcgtctca aaggaccatg caagcagtac caagtgctac tgtaatagta
     2821 accaatccaa cccacttatc tatagcagtg agatatgaaa aagggaaaga ccaagctcca
     2881 gtagtggtgg caaaaggtgc agattattta gcttttaaaa taagggaaat tgcaaaggga
     2941 aatgaaatcc ctataataga aaataaacct attgctaggt tgttatataa gcaagtagaa
     3001 atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt agtagcagta
     3061 tataaaatca aaaatagata caaagtcccc aaaaggtagg ataagtaatg aataagttta
     3121 atttaaagga aaatatggat gcaatagtgg catttggtgt tgtagggatt gttcttatga
     3181 tgatagtgcc attacctaca tttatattag atattttact ggcaattaac ataagtttat
     3241 ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta tctatatttc
     3301 caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca tcaactagat
     3361 tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt tttgttgtag
     3421 gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt caatttgttg
     3481 ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg ttagatgcca
     3541 tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata gatgacaaaa
     3601 ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggt tctatggatg
     3661 gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca gcaataaaca
     3721 taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg ggtgcagcac
     3781 agacatatgt aagacttact ataggagatg gtcttgtaag ccagatacct gctctattaa
     3841 tatctacatc agcaggtata atcgtaacaa gagcttctac tgatgaaaac tttggtggat
     3901 tagtaggtaa acagttaacg gcaataccaa aagcagtaat gatgactggt tgtgttctaa
     3961 tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta ggtataggtg
     4021 ctatagcagc aggttatttt ttaggaagag aaaagaaaga tactttagaa gacttggagt
     4081 ttgaaaaacc tgatatggta aatccagata aaaatgaagt agaagatgta acaaacttaa
     4141 taaatgtaga attaatagaa gtagaaattg gatatggttt aatatcttta gctgatgaat
     4201 cagcaggagg agatttactt cagaggatag catcaataag aagacaatgc gcaatagaca
     4261 tggggatagt cgtacaaccc ataagaataa gagataattt acagttaaat ccaaaccaat
     4321 acaatataaa aataaaagga aatgtagttg gtgggtatga gataatgcct aatatggttt
     4381 tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca attgaaccta
     4441 cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca gagttaaagg
     4501 gttatacagt tgtagattca tccacaatac ttataactca cttactagat ataataaaat
     4561 caaatgcaca cgaattactg ggaagacaag aagttaaaac tattatagat gcagcaagag
     4621 aaaattatag tgctgtagta gatgagttaa taccagattt aatgacactt ggtgaaattc
     4681 aaaaagtgct taaaaatctg ttaagagaaa aagtaaatat aaaggatagg gttacaatac
     4741 ttgagactct tgcagaccag tctagaaata caaaagatat agagcttctt actgaatatg
     4801 taagaatagc cctggctaga agcatttgta ataatttagt tgatgaagat aaggcaatta
     4861 tagtggctac attgtctcca gaaactgaag agctggtatc taataattta caaaggtctg
     4921 taaatggaac ttatccagca gttgacccag aaaatacgaa taaaatattt gaaagtatac
     4981 aagaagtaat gagtaatgta tatttcaatc ataatatacc agtaatagta gtatcaccaa
     5041 agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg actgtattat
     5101 cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg agcatatatg
     5161 atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc caaaatggaa
     5221 ttgggtgaca atattacatt aatagataaa aaagaggtaa gaaaatctgg aataagaggc
     5281 attttttcaa agaaagatat tgaattaact attggatggg aaaagaaaga taatttagaa
     5341 caaaaagatt taaaacgaga gatagaacaa ctaaagtcaa ttataaataa tatggggttt
     5401 gataataaaa atgataatga tatagataaa atatgcaaga atttactaaa cttagagtta
     5461 aatcacgaaa ttgtagaatt tataaaagct gatttacaag aaatgaagtt caatggaatt
     5521 gatacaagta aaaatttagt agaaagtcta aagaaaaaga ttaaaataga aaatcaaaca
     5581 ataaatggga aaattgcttt agttggacca ccaggagtag gtaaaacgac tacaatagca
     5641 aagttggctg ctaaattagt ttttgaggaa aacaaaaaag taggagtaat aactatagat
     5701 acatacagga ttggtgctgt agaacagtta aagatatata ctgatataat gaatattcct
     5761 tttaaagggg taataagccc tgatgagatg gaattggcac ttgatgagat gaaagattgt
     5821 gatgttgtat taatagatac aacaggtaga ggctataaaa actctatgca aatactagaa
     5881 atcaaaaacc ttatagataa agctgaaact gataatatac atttagtttt gaactgtaca
     5941 acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa ttttaaaagc
     6001 ttaataatta caaagttaga tgaaacaata acatatggct caatatttaa cataatgaat
     6061 tatgcacaaa aaccaatatc atatattaca acaggacaaa atgttcctga tgatattata
     6121 aaacctaatg aagataagat tataagatta ctattaggag ttgaaagtat atgatagacc
     6181 aagctcaaat tttgagaaaa atggcaatgg aaaaaagagg acttgatgag attatagtag
     6241 agaatgagaa tacgcctaag ataattacaa tagcttctgg taaaggcggt gttggaaaga
     6301 gtaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa gttcttatat
     6361 tagatgcaga tataggaatg tctaatatag atataataat gggggtaaat gtaaaaggga
     6421 ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca cagactaagt
     6481 atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac tttacagaag
     6541 ctcaaagaaa taagtttata catagcatag aacagattca cgatgtggat tttataataa
     6601 ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt agcactgaat
     6661 ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt ttattaaaag
     6721 ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg gtaattgatt
     6781 tagaagaagc gaaatctaca tacaaaagaa taaaaatggt tgtggacaaa tttctaaacc
     6841 ttaatctaga attgtatgga tacataatag atgacaaaaa agtaggtgta tgtgtaaaaa
     6901 aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt attatagcta
     6961 tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa agttttctaa
     7021 gaaagatgtt tagtatattt aaaagctaag gaggcgtaat taatgaatag agaagaatta
     7081 ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat accagggaaa
     7141 ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga tgcaataaat
     7201 aaatttgata ctgaaaaagg agcaaagttt tctagttact cttatataaa aataaaatct
     7261 gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct aactaaagta
     7321 aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga gccaacttca
     7381 tatgaaattg caaaagaatt aaaggtttca gaaaaagaac ttcatgatat cgaaagtaat
     7441 atagatatgt taaatattgt atcattaaac tatgtaatct ttgaagatac aaatgaaact
     7501 gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat agaagaagaa
     7561 gaaaagttag agattctatc caaagcaatt agtaatttaa atgaaagaga aaaattaata
     7621 ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt gctaggtgtt
     7681 tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag aaacaagata
     7741 aaagaactta aatattctat atagatggtg attgataaga tgatacaaat tatattaata
     7801 gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga atcaaaaaat
     7861 aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa tgatagatta
     7921 ataattgcag aaaaaagaag attcttttta gacaaagtac ttgcagaaaa aagaattaat
     7981 agtcatataa aagtttatga aaattgtaac ctaataaagg ctaatgaaga aagaaaggaa
     8041 aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc tcttaaagtg
     8101 aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct agatattgga
     8161 aagggggaac tgttattaat aaggagttgt tacaaaatat aaggatatta ttatgtgata
     8221 aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgt ataatgcttt
     8281 ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg ttaggcatga
     8341 aatatacaag tgagatgaaa gttttagaat agatgatact gtttagacat ttacaaaaaa
     8401 ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat aactaatcaa
     8461 caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg atataaaagt
     8521 aaacaacttt tgactaaaag ttttgacgaa ttaaagttgg agggttacaa taactacgca
     8581 aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc tatggatgaa
     8641 actatcacaa attttaatca aggacctata aaaactactg ataataaaat ggatgtagct
     8701 attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt ttacactaga
     8761 aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg gtattatgta
     8821 cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa tcaaaaattt
     8881 gaagtggcaa aagataatac aatatcacta gacggaaatg ctatgtataa atttaatatt
     8941 gtagatttta ataactacaa taacttaaaa aaagaaggag ataacttata ttctggagga
     9001 ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc ctctaatgta
     9061 gatatggtat ctgaaattaa taatatgatg acaatatcaa gagaatatga ggcaaatcaa
     9121 aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat tggttcagta
     9181 agataggtaa taggaggtga atttttatgt acacaatgtt ttatactagt aaaacagctt
     9241 tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat tctgattcaa
     9301 caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta ctaaatagac
     9361 catcataccc tacaaatggt aaagatatat cgactggaac aggagctaag gcaacagctc
     9421 ccatgagaaa ttatgaacaa ggtgcattgg ctcctacaga tagtaagagt aatttggcga
     9481 tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca tatacaagaa
     9541 atggtggatt taatgtagat gctttaggta agattgtaga tgataatgga aatatattag
     9601 atgtccaatt tgatgcagga tataactata ataatacaaa tatagatagt acaaacctga
     9661 cagtttcaag aaatggagag ctattcgtag ataataaaaa aataggaaaa attaacttat
     9721 atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattt gtcgcaactg
     9781 atgcagcaca gattaaacaa gttgagaaag tcgatatagt tcaaggctat agagagaggt
     9841 caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga gcatttcaaa
     9901 tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagcgaat aatttaagag
     9961 ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta cgattttggc
    10021 aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt agctgaattt
    10081 tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag tatggttgtt
    10141 gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc ttcgtgcgta
    10201 gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat agatagaagt
    10261 gtagctgata tgtggattga tataacttct ggaggaactg gagttgtaaa ggataatgat
    10321 agagagctta cagaacttga taaaaaagtg cttttacacc ttatggatga tttagtaaga
    10381 aatatgcact tatttgaggg ttttgaaaat atacaagtat tggatacaca tacacatatt
    10441 aatttgccac aattatgttc tccaactgca ccagtatgcg ttgtagattt aaaagtgcta
    10501 aatgataatg agtgtattgg aagtgtttct ctttgttttc catacaatgg tttagaggct
    10561 ctgttagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga tgattcagag
    10621 gaatttaccc aaaaaatcta taatagtgta tctaatatag aattatcagt tattgcagag
    10681 ataggaaaag tatcaattaa tgttgaggat ttacttaaac tagaagttgg agatgtaata
    10741 gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc aaaatcatac
    10801 acagcaacac caggatttat atcaagtaaa aaaggtgtaa aaataaatga tgctgttgga
    10861 aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca aagatgttat
    10921 taaatgataa agttgaaagt gaagttgatg gagatgatga gttagcctta gatgtctctg
    10981 aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta tctgtagtta
    11041 taggaagaac taagatgact ctaagagata ttttaaattt acataaaggt tctcttattg
    11101 aactggatac tttagcagac caagatgtgg aaatactaat agataataag gttttggctt
    11161 atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt atagtaagtg
    11221 aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt aaggcatgag
    11281 gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact caataaaaaa
    11341 gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg tatatcaact
    11401 ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga tatattaaat
    11461 ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga aagtttctat
    11521 aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat gcttacatct
    11581 gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat aaataaagga
    11641 atgtataaag aagctataca agggtttaaa cttataactg accaatactg tgatttaata
    11701 gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat aaaatttgat
    11761 gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa tcaagaaaat
    11821 aacacattag atatgtataa tagtgctaat atacaaacta aaagtaatat aaaagtagat
    11881 gattataaga atgagttctt acataaaagt agtagtaacc ataaaattaa tgattataat
    11941 gatgtaacta aagaaaagag ttcaagcaaa aaccagaaaa gttcagattc taataagaaa
    12001 aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat attattagta
    12061 ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat atctaaaaat
    12121 aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga aaataatata
    12181 gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa aaacattaat
    12241 aaagagaatg atgtaaaatc taaaaaaaat actgtaaaaa atgaagatga taaagattct
    12301 gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc agaaaagaaa
    12361 actaaacctt ctatacaact atataagaaa gttgcagata taggtgaaaa taaagggaat
    12421 acatctgaag caacttatca agttgctatt ctaagtgaaa agttaaaaga ctataaaact
    12481 gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc ttattttgat
    12541 gaatcttatt ataatttagg tatgatgtac tataacaatg gtgatttaaa aaattctaaa
    12601 ttgacattaa agaagttggt taataaggta ccaaatagta tgtataataa ttcaaaagta
    12661 aaagaaattt taaaagaaga atagttaaat tagataagaa aaataaatta ttttatttta
    12721 aaataatatg attataatat attgacatac cattttaaaa aatttaaaat attaattata
    12781 aaatgaggag ggataaaaaa tggaatttga aaaagggagt atttcaattc atacagaaaa
    12841 tatttttcct ataattaaaa aatggttata ctctgataaa gacatattta taagagagct
    12901 tataagtaat ggatgtgatg cagtaagtaa acataaaaga cttgtatcac taggagaaat
    12961 atcagaaaat aagtcatcag attataaaat tacagtatct gtaaataaag gagaaggtac
    13021 acttaaattt attgacaatg gaattggtat gactgaagaa gagataaaaa aatacataaa
    13081 tcaagttgct ttttcaggtg ctgaagattt ctttaataaa tataaggata aaatggaaga
    13141 gtcaaatgat ataataggtc attttggatt aggattttat tctgcattta tggtgtctaa
    13201 aaaggtacaa atagatacac tttcatatac agaaggagcg actcctgtaa gatggatatc
    13261 tgaaggtggt acagaatacg aaatatctga atcagatgct agaaatgata gaggaactac
    13321 tataacacta tttattgatg atgatagtaa agaattttta gatgaattta ctgtaagagg
    13381 aataataaat aaatactgtt ctttcttacc agtagaaata tatttagaag atgttgaaag
    13441 attagaaaga gaagctaaag aagcagaaga aaaagctaaa aaacaaaagg aagatggaaa
    13501 agaagagatt gtagatgcaa aagtaattga gcctttaaac gacactaatc ctctatggtt
    13561 aaaatctcca aaagactgta ctgatgagga gtataaagag ttttatagaa aggtattcaa
    13621 tgtatttgat gaacctttat tttggataca tttaaatgta gattatcctt tcaatttaaa
    13681 gggtatactt tacttcccta aattaaaaaa tgagtttgaa ctgactgaag gaaaagtaaa
    13741 attatacaac aatcaagtgt ttgtggctga caatataaaa gaagttatac cagaattttt
    13801 acttttatta aaaggagtta tagactgccc agacttacca ttaaatgtat caagaagttt
    13861 cttacaaaat gacagggatg taagcaaaat ttctaaacat ataattaaaa aagtagctga
    13921 taagttaaaa tctctttgta aaaatgaaag agaagaatac aataagtttt gggatgatat
    13981 acaaatattt attaaatatg gttgcttaaa agatgaaagt ttctatgaga aggtaaaaga
    14041 atgtatatta ttcaaaacta ttgatgatga gtatataaca ttacaagatt atttagaaaa
    14101 ttgtaaggac aaacatgaaa ataaggtatt ttatgtaagt gataaggaac agcaatctca
    14161 atacattaaa ctatttaaag aatacgactt aagtgctgtt gtcttaaact catcaataga
    14221 tactcatttt atttcattta tggaatataa agagaatgga gttaagttta atagaataga
    14281 tgcagacctt tctgatgtat taaaagataa aaatgaaaat aaagatagtg aagaaaacaa
    14341 agaagagata gctaagatag aaggtctgtt taaagaagct gtcggagaaa gagtgaagaa
    14401 ttattctgta gaaggattga aaaatgaaga cactccagct atggtattag tatcagaaca
    14461 atctattaga atggcagaaa tgcagtcaag atttgctgga atggatttag gaatgaattt
    14521 tgaagaagaa aaaactcttg taataaatga gaacagtcca ataatcaaaa aattggtttc
    14581 tcttaaagat gatgaggaaa agaaagataa gattacgctt atttgtaacc agatagcaga
    14641 tttagcttta ctttctaata aagaattaaa gccagatgag ttagattcat ttgtacaaag
    14701 aagtaataag cttatgagtc ttttgataga attataagat tgaatttgaa tacattgaag
    14761 caaatttata aaaagtattc aatatcagtg ataagaaaga agtgtctcaa aatgaacttt
    14821 tcagttcatg aggcgctttt tttatgtgaa attaaattta ttttaattat ttcaacaatg
    14881 aaaaatatat ttatctctat tttgagatag accattcttt attttttatc tttggttaat
    14941 ttttaaatat ttaagtaggt tctaataaaa aactctattt agttaaatat agttaacttg
    15001 atatataata agataataca tataattaag taaaagattg ttttttagtt tatgtttaaa
    15061 tacataatta cactttgtta aaaacataaa attattacta aagtagaaat aggaggtttt
    15121 agcatgaatt ttaattataa tttgccagta aatatcttat ttgggagagg cagaattaat
    15181 gatgtgggaa aagaagtatc taaatatggc aaaaaagtat tgatagtaac aggtaaaaat
    15241 agtacaaaga aaactggatt acttgataaa acaatagatt tactaaaaga ctctaaaata
    15301 gaatatgaag tttttgatag agtagaacaa aatcctctta taactactat atattcagga
    15361 gtagaaatta taaaatcaac tggatgtgat tgtgttttag ggcttggtgg tggaagcatt
    15421 atggatgctg ccaagagtat agctttttct tataaaaacc caggagattt aaatgattat
    15481 atatttggaa ttagacaagg tgaagaagct ttacctataa tcttagttcc aacaacatca
    15541 ggtacaggta gtgaaggaaa ttgttttgca gtacttacaa attcagaaac taaagataaa
    15601 aaatctataa agaagaatag tatgtatgca aaagcatcaa taattgaccc agaacttatg
    15661 gttacaatgc caaaacatat aattgcatca gttggatttg atgcacttgc ccataatatg
    15721 gaagcatatc tttctaatgg aagaaaccct attgcagatg tacaagctat ttatgggatt
    15781 gaacttatat cagaaaacct aataaaggta tataatgatg ttaatgattt agaagcatgg
    15841 gaaaaaataa cacttgcaag tactattgga ggaatgacga ttggtacagc gggtacagcc
    15901 ttgccacatg gaatggagca tcctgtaagt ggtcttagag atgtaatgca tggaaaagga
    15961 cttgcagctt tgactcctac tattatagaa agttcatggg atagtgatat tgaaagattt
    16021 ggtaatattt caaaggtatt aggaggaact tgtgcagaag attgttcaga tgcaattaga
    16081 actttcctta aaaaaataga tttagatata aaactaagtg atttaggtat tgaaaaatca
    16141 gatatagatt ggttaactga taattgtatg aaaatatcag ttgcaaatat taagagacat
    16201 ccaaagtatt ttaataagga acaaataaag gaaatgtacc ataagtcttt ataatttgac
    16261 tttacatatt tagttttata taaaaattat a
//
LOCUS       DAELJO010000077        16218 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELJO010000077 DAELJO010000000
VERSION     DAELJO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.077"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..237
                     /gene="tuf"
                     /locus_tag="KPB88_002063"
     CDS             <1..237
                     /gene="tuf"
                     /locus_tag="KPB88_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0054341.1"
                     /translation="GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            487..636
                     /gene="rpmG"
                     /locus_tag="KPB88_002064"
     CDS             487..636
                     /gene="rpmG"
                     /locus_tag="KPB88_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG0054342.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            662..883
                     /gene="secE"
                     /locus_tag="KPB88_002065"
     CDS             662..883
                     /gene="secE"
                     /locus_tag="KPB88_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG0054343.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            903..1445
                     /gene="nusG"
                     /locus_tag="KPB88_002066"
     CDS             903..1445
                     /gene="nusG"
                     /locus_tag="KPB88_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG0054344.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1484..1909
                     /gene="rplK"
                     /locus_tag="KPB88_002067"
     CDS             1484..1909
                     /gene="rplK"
                     /locus_tag="KPB88_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG0054345.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1980..2678
                     /gene="rplA"
                     /locus_tag="KPB88_002068"
     CDS             1980..2678
                     /gene="rplA"
                     /locus_tag="KPB88_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG0054346.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2730..2858
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2901..3407
                     /locus_tag="KPB88_002069"
     CDS             2901..3407
                     /locus_tag="KPB88_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG0054347.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3465..3830
                     /gene="rplL"
                     /locus_tag="KPB88_002070"
     CDS             3465..3830
                     /gene="rplL"
                     /locus_tag="KPB88_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG0054348.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4222..5010
                     /locus_tag="KPB88_002071"
     CDS             4222..5010
                     /locus_tag="KPB88_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0054349.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5481..9197
                     /gene="rpoB"
                     /locus_tag="KPB88_002072"
     CDS             5481..9197
                     /gene="rpoB"
                     /locus_tag="KPB88_002072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG0054350.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9239..12724
                     /gene="rpoC"
                     /locus_tag="KPB88_002073"
     CDS             9239..12724
                     /gene="rpoC"
                     /locus_tag="KPB88_002073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG0054351.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13013..13435
                     /gene="rpsL"
                     /locus_tag="KPB88_002074"
     CDS             13013..13435
                     /gene="rpsL"
                     /locus_tag="KPB88_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG0054352.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13559..14029
                     /gene="rpsG"
                     /locus_tag="KPB88_002075"
     CDS             13559..14029
                     /gene="rpsG"
                     /locus_tag="KPB88_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG0054353.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14078..16144
                     /gene="fusA"
                     /locus_tag="KPB88_002076"
     CDS             14078..16144
                     /gene="fusA"
                     /locus_tag="KPB88_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0054354.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6347 a         2020 c         3308 g         4543 t
ORIGIN      
        1 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
       61 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      121 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      181 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaattc
      241 tttactaaac tgatggatta aaaggaataa caaaaagagg ggttatgctc ctcttttttt
      301 aacatagaaa tacaaaaaca ttgacacagc ataggatttt gtgataactt atacaaggta
      361 atgattatag gaagttaata atatcttgaa tactgtagat ttttaataaa tacatatttt
      421 aaagatatta tattcacata tattaacaat atatattatt gtattacaaa gttggaggtg
      481 tatcagatga gagttaaagt aactttagca tgtacagagt gtaagcaaag aaactacaac
      541 actactaaga ataagaaaaa caatccagac agaatagaat tacaaaaata ttgtagattc
      601 tgtaaaaagc atacaactca taaagaaaca aaatagtttt ttataaggat gtgagtatgg
      661 aatggctgcc caaaaaaatg acggtgcaaa aactaaaaaa aggtttagtt tgtttggata
      721 cttaaaagaa actaaacaag aattaaaaag agttacatgg ccgacaaaaa aggaactgtt
      781 taaaaacaca agtatagttt taactgtagt tatttcatgc actatattag tatggggtat
      841 agacactata ttatctggtg cactagcgtt attactaaaa tagacgaaaa gaggtaagtt
      901 aaatgtcaga attacaagaa gcaagttggt atgtagttca tacctattcg ggacatgaaa
      961 ataaagttaa agctacaatt gagaaagcag ttaaaacaag aggtatggaa gactgcataa
     1021 gacaagtagt tgttccaaca gaagaagtag tggaaactac taaaactgga aaagaaaaaa
     1081 ctagacaacg taaagtttat ccaagttatg ttttggttaa aatgataata actgatgaat
     1141 cttggtatgt agtaagaaat actaagggag ttacaggttt cgttggacct ggttcaaaac
     1201 cagtgccatt aagtgaagac gaagtcaaag ctatggggat agacacaaca gaccctaaag
     1261 tagtaaatag cgatattgat tttgaaatag gagatacagt aaaagtatct caaggaccat
     1321 ttagtggaca aataggtaac atagaagaaa ttgacttaga aaatagagaa gtcaaagtat
     1381 gcataaatgc atttggtaaa aggactctat ttgtaataga gcttgaaggt atagaaaaaa
     1441 tctaatacta tattatagaa aaatctgagg aggtgcgctc aaaatggcta aaaaagttat
     1501 aggtcaaata aaattacaaa tacctgcagg aaaggctact ccagcgccac cagttggacc
     1561 agcattagga caacatggtg ttaatataat gggattcaca aaagaattta atgctaaaac
     1621 tgcagatcaa gctggaatga taataccagt tgttataact gtatatcaag atagatcatt
     1681 cagttttata acaaaaactc caccagctgc agttttaatt aaaaaagcat taaacttaaa
     1741 atcaggttct ggagaaccaa acaagaaaaa agttgctaaa atgacttcag ctcaagtaag
     1801 agaaatagct gaattaaaga tgcctgactt aaatgctgca tcagtagaag ctgctatgag
     1861 tatgatagct ggtactgcta gaagcatggg tgttgtaatc gaagactaat taaaagatac
     1921 actttttttt ataaagtggg aggctagaag ccgaaattaa ccacgaggag gaaaataaaa
     1981 tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga actaaatttt
     2041 atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa tttgatgaga
     2101 cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa caagtaagag
     2161 gtgcagttgt attacctcat ggaactggta aaacaaaaag agttttagtt tttgctaaag
     2221 gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct gaagaattag
     2281 ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct actccagata
     2341 tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta atgccaaacc
     2401 ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata aaagctggta
     2461 aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga aaagtatcat
     2521 ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata ataaaagcta
     2581 aaccagctgc tgctaaagga caatatttaa gaagtataac agtagcttct tcaatgggac
     2641 ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga cacaggttga
     2701 caaaaatgtt attatatcac ttgtcaatgt aaaaaagaat agatttgccg tagatagtgg
     2761 gtgcgagagc ttaatatccc gccgaggttt tagataaatt gagattacta aagcttttga
     2821 tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa gcctatttgg
     2881 tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa gttgtttctg
     2941 agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac aaaggattaa
     3001 cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta gattacaaag
     3061 tatacaaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag caatttaatg
     3121 atgaattatt agttggaact aatgccatag catttggata tgatgatcca gttgctccag
     3181 ctagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag atgggtattg
     3241 tggaaggtgc attctatgat gaatctaaga ttgttgaaat ggctaacata ccatcaagag
     3301 aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac ttcgcatact
     3361 taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg cttaactaaa
     3421 aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca atagaacaaa
     3481 tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt aaagctgctg
     3541 aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca gctgctggtg
     3601 gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt ggttcttcaa
     3661 aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa gaagctaaag
     3721 aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa gaagctgatc
     3781 aaataaaaga gaaattagaa gctgctggag ctaaagtaga agttaagtag tttctttaaa
     3841 aagataccca ttgggtatct ttttttattt ataaaaaact ataaagaaag tatttggaat
     3901 tataaagaaa gtatttttat aaaataaaaa aaatttatat aatagtacta aattaagggt
     3961 atcatatatg gtgtaatggt attttaattt aacattatta tcaaattaaa atataaacaa
     4021 ttgttgtaat gtctgtatat aaagtatata attaaactaa aatgtaaaat tttacatttt
     4081 agtttaaaaa aatacacgag tgttttataa ataaaatgta ataaataatt aattaacaaa
     4141 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     4201 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4261 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4321 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4381 ggtggttgtg ctaagtttgt ttactttaat gctcgtgagg aagagacctt tacctcaatg
     4441 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4501 acaaaccctt ctcttgacaa agaccttgta actggagata cagataattt ctttgacaca
     4561 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4621 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4681 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4741 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4801 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4861 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4921 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4981 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     5041 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     5101 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     5161 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     5221 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5281 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5341 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5401 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5461 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5521 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5581 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5641 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5701 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5761 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5821 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5881 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5941 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     6001 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     6061 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     6121 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     6181 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6241 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6301 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6361 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6421 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6481 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6541 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6601 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6661 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6721 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6781 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6841 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6901 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6961 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     7021 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     7081 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     7141 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     7201 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7261 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7321 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7381 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7441 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7501 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7561 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7621 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7681 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7741 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7801 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7861 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7921 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7981 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     8041 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     8101 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     8161 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     8221 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8281 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8341 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8401 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8461 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8521 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8581 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8641 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8701 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8761 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8821 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8881 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8941 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     9001 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     9061 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     9121 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     9181 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9241 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9301 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9361 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9421 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9481 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9541 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9601 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9661 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9721 gtatggatat acttttactg taggaatggg tgctgaagct gtaaagacat tactacaaaa
     9781 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9841 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9901 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9961 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
    10021 tataaataga aacaatagac ttaaaagatt attagagctt ggagctccag atataattgt
    10081 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
    10141 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
    10201 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10261 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10321 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10381 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10441 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10501 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10561 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10621 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10681 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10741 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10801 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10861 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10921 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10981 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    11041 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    11101 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    11161 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    11221 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11281 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11341 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11401 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11461 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11521 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11581 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11641 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11701 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11761 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11821 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11881 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11941 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    12001 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    12061 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    12121 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    12181 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12241 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12301 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12361 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12421 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12481 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12541 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12601 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12661 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12721 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12781 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12841 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12901 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12961 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    13021 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    13081 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    13141 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    13201 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13261 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13321 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13381 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13441 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13501 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13561 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13621 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13681 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13741 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13801 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13861 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13921 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13981 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    14041 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    14101 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    14161 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    14221 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14281 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14341 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14401 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14461 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14521 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14581 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14641 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14701 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14761 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14821 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14881 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14941 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    15001 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    15061 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    15121 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    15181 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15241 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15301 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15361 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15421 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15481 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15541 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15601 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15661 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15721 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15781 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15841 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15901 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15961 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    16021 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    16081 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    16141 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    16201 tctcatctag cacaagat
//
LOCUS       DAELJO010000078        15946 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELJO010000078 DAELJO010000000
VERSION     DAELJO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.078"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            174..1622
                     /locus_tag="KPB88_002077"
     CDS             174..1622
                     /locus_tag="KPB88_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0054355.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNKIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1648..4038
                     /locus_tag="KPB88_002078"
     CDS             1648..4038
                     /locus_tag="KPB88_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0054356.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKIDVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSTLDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4040..4712
                     /gene="pgmB"
                     /locus_tag="KPB88_002079"
                     /pseudo
     CDS             4040..4712
                     /gene="pgmB"
                     /locus_tag="KPB88_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            4751..6151
                     /locus_tag="KPB88_002080"
     CDS             4751..6151
                     /locus_tag="KPB88_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0054357.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGIPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYIFG
                     VEKNVKEGIDNVVEEV"
     gene            6466..7308
                     /locus_tag="KPB88_002081"
     CDS             6466..7308
                     /locus_tag="KPB88_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0054358.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7453..9381
                     /locus_tag="KPB88_002082"
     CDS             7453..9381
                     /locus_tag="KPB88_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0054359.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9442..10869
                     /gene="ascB"
                     /locus_tag="KPB88_002083"
     CDS             9442..10869
                     /gene="ascB"
                     /locus_tag="KPB88_002083"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0054360.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11280..12056
                     /locus_tag="KPB88_002084"
     CDS             11280..12056
                     /locus_tag="KPB88_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0054361.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAIALTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12072..12437
                     /locus_tag="KPB88_002085"
     CDS             12072..12437
                     /locus_tag="KPB88_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0054362.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12604..13353)
                     /locus_tag="KPB88_002086"
     CDS             complement(12604..13353)
                     /locus_tag="KPB88_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0054363.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            13831..14256
                     /locus_tag="KPB88_002087"
     CDS             13831..14256
                     /locus_tag="KPB88_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054364.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            14288..15475
                     /locus_tag="KPB88_002088"
     CDS             14288..15475
                     /locus_tag="KPB88_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG0054365.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPRDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNNTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            15667..>15946
                     /locus_tag="KPB88_002089"
     CDS             15667..>15946
                     /locus_tag="KPB88_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0054366.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYAL"
BASE COUNT         6248 a         1686 c         2845 g         5167 t
ORIGIN      
        1 aatattgata tttaatatga aagtattgaa aatagacaat attaattata gtgagtaaaa
       61 aatataattg gatttttaaa ttgaattatt taaaaggtat agtatgaaaa aaatcttata
      121 actaaatatg agatagaaat tataaaactt tatattgtga aagaggtaga tgcatgaaat
      181 tagggaaaga ttttttatgg ggaggggcaa tttcagccaa tcaaagtgaa ggattttatt
      241 tagatggggg aaaaggttta tctcagtttg ataaactacc attaaatgat aagagattaa
      301 aagatgtatg tttagatgag gataatattt taaatgaaaa taatcaatac tatccaagtc
      361 atatagggat taaattttgt gaaaattaca aagaagatat agcaatgtta gcagaaatag
      421 gttttaaatg ttttagattt tctatatctt ggtcaagaat attcccaaca ggagaagaaa
      481 gtgaaccaaa tgaaaaggga ttacaacttt atgataatat aataaaagaa ttaaaaaaat
      541 ataatataga gccattaata acgataagcc actttgatat accttattct ctagttgaaa
      601 aatattctgg gtgggctgat agaagagtgg ttaatttata tgtaaaatat gctgaaattt
      661 tgatgaaaag atataaagat gatgtcaaat actggattcc ttttaacgaa atgaatatga
      721 taatgcatat tccatatata ggtgctgggt taacttttat agctgatgaa aatcaacttc
      781 agaaaaaata tcaagcagca caccatcaat tattagctaa tgcaaaaact atagaaatag
      841 gaaggtatat aaataaagaa tttaagtttg ggtgtatgtt agctgctgga aaaacatatc
      901 catacacatg taaaccagaa gatgtatttg caactttaga gcatgaaagg cataatttgt
      961 ttttttctga tgttcaagtt aggggtgtgt atccaagctt tatgagttat tatttaaaat
     1021 caaataaaat aaaattaata ataaaagagg aagatttatc aacacttaag gataatactg
     1081 tagattttgt atcttttagc tattattcta gtgcttgtag ttcagctaga gctgaaggtt
     1141 tagaaaaatc aaaggcaaac ggaccagaaa ccataaaaaa tccatatctt ccaaaatcaa
     1201 atagtgtatg gcaaaacgac cctgtagggt taagaataac tatgaatcag ttatatgaaa
     1261 ggtatcagtt acctttattt atagtagaaa atggactggg tactcaagat attgtaaatg
     1321 aagatggaaa aataattgat gattatcgta tagattacat gaaagaacat attaaaaata
     1381 tgattcttgg agtaaatgaa gatggaatag agcttatagg atatcttatg tggggatgta
     1441 tagatgtaac cagtgtaagt gaaggaaaga tgacaaaacg atatggcata attcatgttg
     1501 attctgatga ctatggaaga ggctcattta aaagaactaa aaaacaaagt gctttttggt
     1561 ataaaaaagt aatagaaaca gatggagaaa ttttatttaa tgatatggaa actcatgcat
     1621 aattcatttt aagaaaggag tgttattatg agtttaatat ttactactaa ggaccaactt
     1681 atagagcaag ggtttagtaa ggatacaata aaatccaatg aaacgctgtt tacactagct
     1741 aatggacatt taggagttag agggaatatt gaagagtctg attttaataa tgagtatgta
     1801 gataatatgg gtacatatgt aaatggattt tatgaagaat ctccaattac atatggagaa
     1861 agcgcttatg gctatgcaaa acaaaatcag actatctgta agttacctaa tgctcatata
     1921 gtaaatttct ctattgaagg agattatttt gatttatcaa aaggtattac aagtgaacat
     1981 actaggatac tagatttaaa tgaagggatt cttaaacgtt catttatatg ggaaaactct
     2041 tgtggtagaa aagttaaagt atctatagaa agattagtat cacaagatat agaagaatta
     2101 atgatgattt catatacaat aacaccattg aattttaatg gaaaaataaa atttaaaaat
     2161 aaaatggaaa atagtatgga gaagtttaat tgtgaaaaag tagatgaccc aagagtggca
     2221 tctataaata agaagcaatt taaaattgat gtaaaaaaat ataacaataa gtattttatg
     2281 aatttagtta cagaaagaag tagattaggg ttatggtgtg ggtttgattc atatattact
     2341 tgtggaaata ttacaaatga gacctataat gtttcagatg aatgtattga gcgagaaata
     2401 gaagtctatg ccaaaaaaga tgaaaggtta aatttagaaa ttgttgtagg ctatagtaga
     2461 gtatattctg atttaaatct aaatgaagaa cgtttaaaat cattagaatg tctggagtcg
     2521 ttaaaatgtt tagaatcaga agcacattta aaatcattag aattggtttt aagagaagca
     2581 tgtaatattg gatttgataa agtcaaaaat attcagagaa agaaaatgaa tgagttttgg
     2641 acaacctctg atgtagagat tgaaggcgat aaaactcttc aactggggat aaaaaccaat
     2701 ttatttcata tttatcaatc agcaggaaga gatggaaaga ctaatatatc agcaaaaggt
     2761 cttacaggtg atggctatga ggggcattat ttctgggata cagaaatgta tatattacca
     2821 ttttttatat atactcaacc atccattgct agagctttat tagattatag atactcaacc
     2881 ttagatgcat caagagaaag agctagagaa cttggaataa ataaaggttg cttatattca
     2941 tggagaacta taaatggtaa tgaatgcagt gcatattatc cagcaggaac agcacagttt
     3001 catataaatg cagatatagc ttatggtatt agaacgtatt ttgaagcaac taatgatgaa
     3061 gaatttatga tgcaaaatgg cttagaaatt cttatagaaa cagcaagatt ttgggttgaa
     3121 tttggagatt atatagaatc aaaaaataat aaattctgta taaatggagt aacaggacca
     3181 gatgaatata cagcaatagt aaacaataat tactacacta atttaatggc taagtataat
     3241 ttaatatatg cagtacagat ggtagataaa tttaagcata atttattttc aaatgaagta
     3301 tttagaaaag ttaaattaca agaatatgaa gtgaaaaact ggataaatgc cgctcaaaat
     3361 atgtatttac catatgatga agagaagaaa ataagcaaac aagacgattc atttttagaa
     3421 aaatcagtat gggattttaa aaatacacca aaagaaaaat atccattact tctccattat
     3481 catccattaa caatatatag atatcaagta aataaacaag cagatactgt actttcagcg
     3541 ttgcttttcc ctgatgagtt tagcttagaa caaaagaaga gagattttga atactatgaa
     3601 aaaataacta ctcatgattc atctttgtca aggtcaatat ttggaatgat ggcaagtgaa
     3661 attggaaatc atgaaaaagc ttataactat tttatggaca cagcattaat ggatattaat
     3721 aacctacaaa aaaatacaag tgatggaata catgctgcta atatgggtgg aacatggatg
     3781 agtttagtat atggatttgc aggaatgaaa ataaaaaatg ataaattaag ttttgaacca
     3841 aggataccta aacactggaa aaatattaaa tttaatatat tatttagaga taatttagtt
     3901 tctgtaaata tagaagaata taaatcttat tataagttaa tcaaaggtag aaatataacc
     3961 atattacatg ataataaaga aataaaatta tcaaagaaca taatagaagt taataattgt
     4021 tttaaaggga gtaattaata tgaaagcagt cttatttgat cttgatggag ttataacaga
     4081 tactgctaag tatcattttg aagcttggca tgagatagca aaatcgctag atatagaaat
     4141 agatgaaact tttaatgaaa aattaaaagg cataagcagg gaagaatcat tagataaaat
     4201 attgttatct gctaataaag tagatagtta tactaaagag gaaaagttaa aggtgtgtga
     4261 agaaaaaaat aacatttata taaagttaat aaactcatta acatataaag atatattacc
     4321 agggataaat gagcttataa aagaacttaa aaaaaaataa tgtgaaattg ggaatagcat
     4381 ctgctagtaa gaatgcacct aggatattag aatctttggg aataaaagat gaatttgatt
     4441 gtatagtaaa tccatcctta ttagaaaggg gaaaaccata tcctgatatt tttattgaag
     4501 ggagcaagat gctaaattta aaacaatatg attgtgtagg aatagaagat tcttattcag
     4561 gtattaaagc tataaatgct gccaatatga catcaattgg tgtggggaaa aaagaagtac
     4621 taggaaaatg tgaccatatt gtagaagata ctagcttatt aaattacaat acactattac
     4681 aagcttggag taaaaagatt aatttggctt aaaactagat taaaaattat tttaaaagag
     4741 aggataaggt atgaagaata aatatgaaga tgtagctcaa ttgataattg atggacttgg
     4801 aggaaaggat aatataattg acttaactca ttgtatgaca cgattaaggt tcatcttaaa
     4861 agatgaaaag aaagtcaatg cagataaatt aaaatctata gataaggtag ttggagtaaa
     4921 ctcaacttca actcaatatc aggtcataat aggtaatgag gtaggtgttg tatttaatgc
     4981 aataataaat aaggggataa ataccacagg agatattaaa agcgatataa aaaataaaaa
     5041 taaaaaaaat agtattttaa ataatataat agatacaata actggttgta tgacacctat
     5101 gataccagcc cttacagcat ctgggatgat aaaagttata ttatctcttt gcaccacttt
     5161 taattggcta tcatcaaatt caagtacata tagagtatta gatataatag gagatgtagc
     5221 attttacttt atgcctataa tacttgcaat aaatgcagct aaaaaattta atgttaatac
     5281 atcaatagca gttatagtag taggtgtgtt tttacatcca aatttctctg catgggtatc
     5341 aagtggggac cctatatcat ttataggaat accaattcaa ggtgttatat atgcagcatc
     5401 tgttatccct gcacttttaa cagtatggat gatgtcatat atagaaaaat tcattgataa
     5461 attaacacct agcatgttaa aaacaatttt aaatccaact ttagtattat taataagtgc
     5521 gccaatagca ttaatagtaa ttggaccaat aggaaatttc ttaggagagg gattagctag
     5581 tataataaat ttactacaag gaaggttagg atttataatg gtttgtttat tagcagctgc
     5641 aatgccattt atcgttatga cagggatgca tcatgcattg actcctatat tcatatcagc
     5701 atttgcaact acaggacaag aatcgctaat attaatatct caagtttgtg ctaatttagc
     5761 gcaaggtggc gcgacattag ctgtagcatt aagaagcaaa gatagtaaga tgaaacagtt
     5821 agcatcagct gcatggatat cagctattat gggtataact gaaccagctc tttatggagt
     5881 tacattgaaa cttaaaaaac ctgctatgtt agcatctata ggagcaggaa tcgcagggtt
     5941 atttgcagga attactcatg tgactttata tgttccacaa aatagtataa tggcagtact
     6001 tggattatca ggggaaaagg ggatttcaaa tatagtaaat ggacttatag ttatatttat
     6061 agcattggtt gctccattta ttatgacata tatttttgga gttgaaaaaa atgttaagga
     6121 aggtatagat aatgtagtag aggaagtata atattaacta tttacattaa tagaattttt
     6181 attattattg actataactt gttttgtatt tatattgatg caaagaaata agttaatatt
     6241 aataagaata attattagaa ctgacaaaaa gcaacataaa agattgatga agtatggaaa
     6301 taaaaagttg acaaaatatg tctgatgttt tatcatatta attggaatta ggtagtaatt
     6361 gtttaagtac aagctaagct tattcattaa taatagcagt atcctaggaa ggagtatgcc
     6421 cattattatg gataagcttt ttttatgctt aaaaggagga taatcatgat aattaataag
     6481 atattaaaca ataatgttgt aatcacatta gatgataatg atgaagaagt tatcgttatg
     6541 ggtaaaggaa ttggttatca aaaatcaaaa ggcaatctaa tagataaaac taaagtaaat
     6601 aaagtattta gaatatcaaa taaagaaata tcaaacaaac tacaagaatt actcaataat
     6661 attcctatag aacacatgaa attatcaagt gaaattatag aatacgcaca aataaagtta
     6721 aacaagaagc taaatgaaag tatttacata tccttaagtg accatacata ttctgcaatt
     6781 caaaggatga aagaaggaat aaatgtaaaa aatgcaatcc tttgggaaat taaaagattt
     6841 tataaagaag agtttgaaat tggaatgaaa gcactagata ttattgaaaa caaaacaggt
     6901 ataaaattac cagaagatga ggctggattt atagcctttc atatagtgaa tgctcagtta
     6961 agtgaaggtc atactttggc atctgatatt acgaagttaa tacaagaagt acttagcata
     7021 gttaggtatc attttaggat agaatttcat gaagaatcag tgttttatta tagatttata
     7081 atgcatttaa agttttttgc gcaaagattg cttttagata gtgcacatga aggtgagaca
     7141 gacaaagaat tactaagcat tattaaatct aaatataata aagagtttga atgtgtagtt
     7201 aaaattaaaa atttcattaa aaaacaatat aactacatat taacagatga tgagcttata
     7261 tatttaacaa tacatttagc taaagtagtt aaagactcaa atatgtaagt gaaataattt
     7321 ttcagtagta tattaagaat gattgaaagg agtgatacta acaaaattat acttatggtt
     7381 ggatggtaac attgagttgg ccaaaccttc aaatttcaaa aaattaattt aaacgaaata
     7441 tggaggagaa aaatgggaaa atacaaacaa ttagcacaag agatagtaaa caatgttggt
     7501 ggaaaagaaa atattagtgg gttagtacac tgtattacaa gattacgttt tactttaaaa
     7561 gatgaaagta tagcaaatga caatgtatta aaaaacatgg aaggtattgt tactgtaatg
     7621 aaaagtggag gacaatatca agtagttata ggaaatcatg ttgaagctgt ctataaagat
     7681 gtagttgaaa taattggttt agatgattca agtacaagtt cagaaactaa aaaatcaggt
     7741 aatatactag ataaaggtat agatataata tcaggaattt ttcagccagt gttaggaata
     7801 atggcagctt gtggtatgtt gaaaggattt aatgcattat ttgtagcaat gggattatat
     7861 agtgatgtat ctggtggtta tatggtaata aatgcagcag gagatgcatt atttacattc
     7921 cttcctttgt tcttaggata tacttcagct aagaagtttg gattaaaacc aatgttggga
     7981 ttagcactag gagctgcagt atgttatcct gctatacaag gtagcagtat atcagcagga
     8041 gcagatgtga tgtacacttt atttaaagga acaatgtttg catcaccagt atatattgat
     8101 ttctttggac taccaattgt atcaattgat tatactggaa cagtcgtacc aattatattt
     8161 atagtgtatt ttgcttctag gtgtgaaaaa ttatttaata agtgtgttcc agatttagtt
     8221 aagtttttct ttgtgccaat gcttacatta ttaataacag tgcctgttgc acttatagtt
     8281 ataggcccta tagcaacatt tggttctaca cttatttcac aaattgtaat ttcaataaga
     8341 gattttagtc cactattggc aggtgctata gtaggattta cttggcaaat tcttgttatt
     8401 tttggtatgc actggggatt tattcctgta tatataaaca atgttatgac tttaggatat
     8461 gataatgtta tgatgccatt ctttgcctgt acatttgcaa gttcggcagt tgtgttggca
     8521 atatttttca agactaaaga taaaaaacta aaagagatgg caataccaaa ctttatatct
     8581 ggtatatttg gagttacaga gccagcaatt tatggaatat tattgccttt aaagaaacca
     8641 tttataataa gttgtattgc tagtggtatt ggaggcgctt tctatggatt ctttaatctt
     8701 agaaaattta taactggagg aatgggaata tttgaacttc cagcaatgat tgaaccagat
     8761 gggggaatgg gtaacttaat agtagctctt tctggtatag ctatatcaat ggtcgtagct
     8821 tttgtattaa ctatgatttt atataaagat gagaaagtag aagagtctaa aaaagtaaga
     8881 aataagggta aagaagaaat aaaagaagtc aagtctacta aattagaaag agaaatagta
     8941 acaagtccta taaaaggtga agtattaaaa ttaagtgata tagaggatgc agcatttgca
     9001 tctggtgtac taggtcaagg ggtggcaata atacctagtg atggaaaagt agttgctcca
     9061 gtagatgggg tagttacaac cttatttcct agtctacatg ctatagggat tctttctgat
     9121 acaggtgttg aagtgcttat tcatattgga ttaaatacta ttcaattaga gggaagaggt
     9181 tttgaggcat gtataaaaca aggtgataga attacaaaag gtcaaactat attaaacttt
     9241 gatgttgatg caataaaaga gttggggtat agtactgtaa ctccaattgt tattacaaat
     9301 tcatcacaat ttttagatgt agtagaaaca gaaagtaaaa atattgaatt agaagacaat
     9361 ctaattacag tcttatttta gtaaagattg aggtattagc ataatctagt gctttggtta
     9421 taattttagg aggctttgac tatgagtttt aagaatgatt ttttatgggg aggagctact
     9481 gcggccaatc aatgtgaagg tggatacaat gaagacgaaa gaggattggc aaatgtagat
     9541 gtatgtccaa caggaaaaga tagaacagca gttattacag ggaaactaaa aatgtttgat
     9601 tttgatgatg agcattatta tccagctaaa actggtattg atatgtatca taattataaa
     9661 gaagatataa agttatttgc tgaaatggga tttaaagtat atagaatgag cattgcatgg
     9721 agtcgaatat ttccaaaagg tgatgaagaa acaccaaatg aaaagggact tcaattttat
     9781 gaaaatatat ttaaggagtg taaaaaatat aaaatagagc ctttagtcac tattacacac
     9841 tttgattgtc caatgcattt agttaaaaag tatggtgcat ggcgtaatcg aaaaatgata
     9901 ggtttctatg agaaattatg caatgttatc tttagaagat ataaaggatt agtgaagtac
     9961 tggctaactt tcaatgaaat aaatatgatt ttacatgctc catttatggg tgctggtatt
    10021 tgctttgaag aaggtgaaga tgttgaaaag ataaaatatc aagctgccca tcatgagctt
    10081 gtagctagtg caattgcaac taaaatagca catgaagttg attcaaataa cttaattgga
    10141 tgtatgtttg cagctggaag tgtatatcct tatagttgta atcctaatga tgtatgggaa
    10201 gctacaaagt tagatagaga aaattacttc tttgtggatg tacaatctaa aggtaaatat
    10261 cctaactatg cgttaaagta tatggagcaa aaaggtatta caccagaaat ggaacctggt
    10321 gatattgaat tattgaataa atatacagta gattttattt cattttcata ttacaatagc
    10381 cgttgtgtta gaaccgatga aaatgcagat gatatggctg aaggaaacat atttacatct
    10441 gctaaaaatc catatctttc atacagtcaa tggggatggc caattgaccc attaggtttg
    10501 agaattacac taaatcatgt atatgataga tatgaaaagc cactatttat tgtagaaaat
    10561 ggattaggtg ctaaagatat agctgatgaa aatggttatg tagaagatga ttacagaatt
    10621 gattatctaa gagaacatat aaaagctatg catgatgcag tttctataga tggggtcgat
    10681 ttattaggtt atactacatg ggcacctatt gatttagtaa gtgctggtac aggtgaaatg
    10741 gaaaagcgtt atggatttat atatgtagat agagacaata gtggtaatgg aagtctaaga
    10801 agaatgaaaa agaaaagttt tgaatggtat aagaaagtaa ttgcttcaaa tggtgaagat
    10861 ttatattaga caagaaatct atgttaaata agaactttgt attaaataag aagagtataa
    10921 aaattaataa taagtataat ttttaacttt tttaactata gaaaatcaat acagaattat
    10981 tttacattgt atttatacaa tagtatgatt cacaaataaa attttagaaa ataaaattgt
    11041 taagaagtat gggaagtaag tgcaatttac tttgtaataa aagatgaaaa aggcaagctt
    11101 aggttttaag cttgtctttt ttattatgta taaactatga tattttaaaa ttctatgaaa
    11161 aatttattta atagagaaat taaaaattta atggtaatca aattctttac caatgaatat
    11221 aaaaaatgtc tacgtgttat aatagtttaa actaataaac gaaaggtaga tgaatgaata
    11281 tgaaatacca acatataata gaagttgaca aagaactttg cattggatgt gggctttgta
    11341 aaaatgattg tccagtaaac aatataatta tagaaaataa aaagtcagta attaaaaaac
    11401 aagattgtct aatgtgtgga cattgtgcag caatatgtcc cacaaaagct attgccttga
    11461 caggatttga tgaacctcct atagaactta caaataagcc aaagctagat tcagatgaat
    11521 tactaatggc aattaaatct agaagaagta tacgtaaatt taaagacaaa gaagtttctt
    11581 cagaaattgt aaagcaaatt attgaggcag gtaggtatac ccctagtgct aaaaattcac
    11641 aagatgtatc atatattgtt ttagacaata aaaagtcaat ttatgaaaac gaagcagtga
    11701 aattttttag aaaaataaaa cctattgcaa atatagctat taaatattca aaagaagttg
    11761 agattgacga taactttttc tttaagcatg ctcctattgc aataatgata attacaaaag
    11821 ataagattag tggttcactt gcagcatcaa atatggcatt aatggcagag tcttatggcc
    11881 taggtgtttt atttagtgga tatttttcag atgtagcaaa taattcgcca aagttaaaaa
    11941 agcttttagg tctaaaacgc agtaatcatg ttcttacaac attagtaatt ggatacccag
    12001 atgttaaata tagacgtact gcacagaaag aagtagcaac tgttagatac ttataagaga
    12061 gggtggattt tatggaaaaa gaatgtcaac aaagaatgca agaaataacg tctaaattta
    12121 aagaatgtcg caaagctatc tctgctatgg gtgatgagac tagacaattg attttaattg
    12181 ctctcttaga gagtgatttt aatggtatac gagtgggaga aatcacaaaa aaaacacatc
    12241 tttctcgtcc tgctgtatct catcatctta agatattaaa agaagcagag attgttaatt
    12301 tgcgtagaga aggaacgaaa aattattatt atcttgattc aaaaaaatca caatggaagt
    12361 cactaacaga gctaataaat cttatatttg ttggaataca acatattagt gaaaatgata
    12421 taagaaaagg tgattaaatt atacttgatt taatacaaaa aagattgatt taggaactaa
    12481 aaagttcact ttgagacact atcttttgaa ctatacccat cttaggagac agttaaaata
    12541 tgtaaaactg tataaaaaag tgggtatagt tcattttata atggtttaaa tattaagaat
    12601 atattattct aaatagtatc tttctctgtt ttgagtttta tttttatact caatagcttc
    12661 agtggggcaa ccacaaatac atgccataca atgagtacag ttatttttcc aaactggttt
    12721 tttgtttttt aaattaatat tatttaaagg acatagctct acacattttc cacagccaat
    12781 gcatttatca gtataatgaa atcctttggc actaataatt gtcttataaa aaactgtatt
    12841 tattatacca cttttaattg tacctcctaa gccactagga gtaatagttt caaattcatt
    12901 ttcattgcgt atatcagaaa taatcctata tatttgttta tctgcttctt taatcatttg
    12961 ttttgatgtt tctttgtctg ttgtagaaaa taaagctata taattctctg gcattttaat
    13021 ttctgccata ccttttaatt gccattcctt gtatttacat agtttttgaa tatagtttat
    13081 ggattttgct gtatctcccc cacaagtcat tacaaagtaa actctgttgc ttcctagaaa
    13141 ttttgtttct tttataaaat ccgtaaccac tattggcaat ctccatgcat aagtaggaca
    13201 tacaaatatg aatggtttat ctgctgatat taattcatct gttttttcct cttttataag
    13261 ttgatttagt gatatcagtt catcatttaa ttcttgtgca attttacgag ccacatatcg
    13321 gctattacca gttccgctaa aatataaaat cataataata tctccctaac tattttttat
    13381 tttaactctt aattttttat ttattatagt aatttattga tatattctat tcaaatgtat
    13441 gtaaaattta gtagttaaga aaattataac acattagtaa tttttatact tattggtaaa
    13501 gaatttgatt accattaaat ttagcaacaa gacatcaact ggacttgtat atgagaaggg
    13561 ataataattt gcttatgaaa gattataaat ataaaggtta ttttgaaggt gtagaaatat
    13621 ttgataatgt aaatgacctt aacttaaata ctttaacaac agttagtaga tttgtgcttg
    13681 tttttttgtt tacgataact taaggaaata tgttacctga acaataaatt tatataataa
    13741 aagtatataa atatatatat gaatgttaag attagttgat agattttaaa taataaatcc
    13801 aatattataa acataggagg tatgaaaaat atgattaatg agaatttaaa aagtctaaga
    13861 aaaatcaacc agtatacaca agaagaatta gctgaaaaac ttaatgtttc tagacaatca
    13921 atagcaaaat gggaaagtgg agaatctatt cctgatatag gtagttgcat taaacttgca
    13981 aaattgtaca atgtcaaact agatgactta gttaatcatt cagaagaaaa aacaggtatt
    14041 atagtacctc ctaaaggaaa gtttttcttt ggagcagttg tagttggtga acgtggtcag
    14101 attgtcattc caaaagaagc aagagaagta tttaatataa atgcaggaga taaattattg
    14161 gtacttggag atgaggaaag aggattagga attgttcatc aacgtgattt gataaatttt
    14221 ataggtcatg tgggagtagt acgtaaagaa gaatgattta aaaaaattat aaaaggaggt
    14281 atgaaatatg agtaaaatag tattttttag cattcctgca tatggtcata ctaatcctac
    14341 tattgaagta gttcgtgaat tagtggatag aggaaatgag gtattgtatt attcttttaa
    14401 tgaatttaaa gataaaattg aaggtgctgg agcaaaattt atttgttgtg ataaatacct
    14461 tccagaatta ctccctagag atgagaaaaa aattggtaag gattttcctg gattaattga
    14521 gatgatagta gatactacta tttctttaga tgaaaaagtt tgtagggaac ttaaagattt
    14581 taatccagat tgcattgtat cagattcatt aagtttttgg ggaaagttat ttgcacaaaa
    14641 acttaatatt ccatatgtat gctctacaac aacttttgca tttaataaac atacagcaaa
    14701 gttgatgaaa caaaacttta tagaaattat acgtatgatt tttggtgtaa gacgcattaa
    14761 taaaaaaata aagcttttac aaagaaaagg gtatgaagta aaaaatttta tttctatagt
    14821 ttctaatgat aataatacgg ataccattgt atatacatca aaagaatttc aaccaatggt
    14881 agaaaccttc tctagtaagt attcatttgt aggtccatca gtatcaaagt tgattattga
    14941 acctaaagag agaaaaagaa agctaatata tatttcgctt ggtacaatta ataataaaaa
    15001 tgttagcttt tataaaaact gtataaatgc atttaaagat tctgatgttg atgtgattat
    15061 gtctgttggt agaagtactg atattaaaag tttaggaaat ataccaaata attttgaggt
    15121 taaaaatagt gttgaacaga ttgctatact tcaaaaaact gatgtgtttg ttactcattc
    15181 tgggatgaat agtgttaatg aaagtttata ttatggagta cctatggtat tatttcctca
    15241 acattcggag cagagaatgg tagctaagag agttgttcat ttgggagcag gaattatgtt
    15301 aaaagaggat aagtcagaaa gtattaaaaa agcagtattt caagttataa atgacaatga
    15361 atataaagaa aatgcaacta aactatcaaa aagcttttat aatgcaggag gctcaaaaaa
    15421 agcagcagat gtaatattac agattatatg taatagtaga caagttgaca tataatagat
    15481 ttgttaaaaa tatatataca tacatgtaat ttaatcaaag tactgacagt ggggtctttt
    15541 aacctaccag ttggggcata tatattgatg ctaaatatga gggatgttat attttaataa
    15601 aaaagataac atcctttttt tagtgcatta tatcgagttt taaataataa atgaaggagg
    15661 attaatatga aaaaatataa agttattttt atagtattaa ttataggtat tataagtgta
    15721 acagggtttg ttgtatataa aaactgcatt aaaccatgga aagatgctga aacatatatt
    15781 gataagtata tggtaaaaca agatatttca aaagataata taaaaagtat tacaaaagaa
    15841 aaagccaaga aggcaagtta cgaaggaata ctatacaaag tgtattacaa agatgaccct
    15901 cgatatgagt atcaatattt ttatagtgat aattattatg ctctat
//
LOCUS       DAELJO010000079        15389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELJO010000079 DAELJO010000000
VERSION     DAELJO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.079"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(167..389)
                     /locus_tag="KPB88_002090"
                     /pseudo
     CDS             complement(167..389)
                     /locus_tag="KPB88_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
     gene            complement(429..611)
                     /locus_tag="KPB88_002091"
     CDS             complement(429..611)
                     /locus_tag="KPB88_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054367.1"
                     /translation="MNINEVVVRILAERVLNKGLSPLKNRPFELDDVTNEDYRKAVED
                     YIIKESGVVEGAKPTV"
     gene            complement(611..904)
                     /locus_tag="KPB88_002092"
     CDS             complement(611..904)
                     /locus_tag="KPB88_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054368.1"
                     /translation="MNRANRIICDQTGKILLQTGEAMGDVLEHDEITELHCIDVEYGS
                     IDYTKNRIIGINIETKEPILEEIPVYLTEEEKRIKELENQILLNENEKVGGLL"
     gene            complement(918..2642)
                     /locus_tag="KPB88_002093"
     CDS             complement(918..2642)
                     /locus_tag="KPB88_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054369.1"
                     /translation="MQTVWNFDYANYVRSISLLPGRYKLECWGACGGGKGASSFNECA
                     KGGYAKGEIILKKRTNLIVCVGQSGYEKLAKDSNITRTGFNGGGRAGSTNIGDYIYAG
                     YGGGATDIRLYYSSTTWDSSEGLLSRILVAGGAGAISGFSYPHPSIGHGGGEKGSDGV
                     SANTNRFSGGGSQYQGGSNQETIEYHGSFGKGGIGYYSVGGGGGWYGGGGTNAGNVAG
                     GGSGYALTKDSYKPPGYIPTSEYWLENVVMTTGGNTTRADGYAKITLLQALPFLNISS
                     YNSTQVTFKADHTDPTLLTKIEVFIDDTLKETITTDLTTEKTINYTLEDNALHTLKIV
                     VTDSNNATAEKVLSISKGIAPLPAGSTTDEVTNKWIEIKDTFRSGKTSIINTLALKNI
                     EASLNNTLVELSEKIKVGFDSKDASLQDLIKQLTQANNTISQLNTKYKVASGTTTALE
                     AEGRGSLFEVTYSGGKSYAYNRWVKVTGLNFTPNIFITFENHKTSYPYFYCNFTFACQ
                     GVFDKDFAVVTFYSFRGQPGETTYDATGNVIHIDKGAPYMNENGVYLPVYTQEGYLTR
                     NWYAIKFK"
     gene            complement(2661..3440)
                     /locus_tag="KPB88_002094"
     CDS             complement(2661..3440)
                     /locus_tag="KPB88_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0054370.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSAYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREVGVFDSKDQLL
                     AISSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVALATLKDIQDLDTKI
                     DTTKAELTSNIETAKTELNNKIGDTTLLTTTDKTNIVSAINEVKTSVDSIETTAEKTS
                     YNNATSNLNATNVQGAIDEVVRKIENFNEININIQNDMLPI"
     gene            complement(3441..4058)
                     /locus_tag="KPB88_002095"
     CDS             complement(3441..4058)
                     /locus_tag="KPB88_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0054371.1"
                     /translation="MKLIDKLPSFYNNDITSKIQEAYDIELETLRETYDDIFDQFFVD
                     TATWGLDYWENILSIKNRFDLSIEDRRSNIKAKMRGKGTTTIEVIKVIGEAYTKTDVD
                     VKVFSTLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFEMILYNQNGLYAGAM
                     TSTGETVTIYPYTPKNIECFGEIILASGNNRSAERVTLYPKQEVI"
     gene            complement(4058..5104)
                     /locus_tag="KPB88_002096"
     CDS             complement(4058..5104)
                     /locus_tag="KPB88_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0054372.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVEGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDSEVIERCKEHIEEEMPIGSTLTVATPSPLNISISATIKLEVGYT
                     LDFVKESFLESINSYLININKEIIYTKVSAILASVEGLHDFSNLLLNNKAENIVFEED
                     KVPSVTTLEFSEVV"
     gene            complement(5097..5525)
                     /locus_tag="KPB88_002097"
     CDS             complement(5097..5525)
                     /locus_tag="KPB88_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0054373.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(5525..5851)
                     /locus_tag="KPB88_002098"
     CDS             complement(5525..5851)
                     /locus_tag="KPB88_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG0054374.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTEINQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(5867..7396)
                     /locus_tag="KPB88_002099"
     CDS             complement(5867..7396)
                     /locus_tag="KPB88_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0054375.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLRAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(7678..8100)
                     /locus_tag="KPB88_002100"
     CDS             complement(7678..8100)
                     /locus_tag="KPB88_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054376.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(8100..10553)
                     /locus_tag="KPB88_002101"
     CDS             complement(8100..10553)
                     /locus_tag="KPB88_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054377.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSVLGKLKRVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(10553..10720)
                     /locus_tag="KPB88_002102"
     CDS             complement(10553..10720)
                     /locus_tag="KPB88_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054378.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(10747..11193)
                     /locus_tag="KPB88_002103"
     CDS             complement(10747..11193)
                     /locus_tag="KPB88_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0054379.1"
                     /translation="MSENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(11319..11747)
                     /locus_tag="KPB88_002104"
     CDS             complement(11319..11747)
                     /locus_tag="KPB88_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0054380.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(11761..12825)
                     /locus_tag="KPB88_002105"
     CDS             complement(11761..12825)
                     /locus_tag="KPB88_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0054381.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIN
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVVEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(12830..13273)
                     /locus_tag="KPB88_002106"
     CDS             complement(12830..13273)
                     /locus_tag="KPB88_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054382.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(13522..13962)
                     /locus_tag="KPB88_002107"
     CDS             complement(13522..13962)
                     /locus_tag="KPB88_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054383.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(14067..14264)
                     /locus_tag="KPB88_002108"
     CDS             complement(14067..14264)
                     /locus_tag="KPB88_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054384.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            14433..14753
                     /locus_tag="KPB88_002109"
     CDS             14433..14753
                     /locus_tag="KPB88_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0054385.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            14835..15341
                     /locus_tag="KPB88_002110"
     CDS             14835..15341
                     /locus_tag="KPB88_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054386.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         4564 a         2910 c         1711 g         6204 t
ORIGIN      
        1 tcaagtttaa gctcaactcc tttttgaatt tttatatatt atttttatta cttttttatt
       61 gatattttaa actataaaat ccttctcaat aaaatagtac actatataca atactacttt
      121 atctttttat aacaatatat catccatctt ttatttttct aataatttaa aatatttctc
      181 gatgaataac aaatataaag aatcttacta atcattgttc taattaggca tttaaacata
      241 cttataagtg aatttaagtt ctcacacagt gcttttaatt cttttttaga ctctatattt
      301 gctatcttta attcgtctat ttcatcatta tgtttattta ttgttacctc atgtcgtttc
      361 aaattctctt tgaaaagttc ttcattcatg taagccgcct gatttttgta ttaaaaaaag
      421 aacattacct atactgtagg ttttgctcct tctactactc cactttcttt aattatataa
      481 tcttctactg cttttctata atcctcgttg gttacatcat ctaactcaaa aggtctgttt
      541 tttaaagggc ttaatccttt atttaaaacc ctctctgcta atattcttac aacaacttca
      601 tttatattca ttataaaagt cctcctactt tttcgttttc attgagtaat atttgatttt
      661 ctaattcttt tattctcttt tcttcttctg ttagatatac tggtatttct tctaaaattg
      721 gttcttttgt ttctatattt atacctataa ttctattttt agtatagtct atacttccgt
      781 actcaacatc aatgcaatgt aattcagtta tttcatcatg ctctaaaaca tcccccattg
      841 cttctcctgt ttggagtaat atttttcctg tttggtcaca tataattcta tttgctctat
      901 tcattttatc acctcattta tttaaatttt atagcatacc agttgcgagt taggtaaccc
      961 tcttgagtat aaacaggaag ataaacacca ttttcattca tataaggtgc tcctttatca
     1021 atatgaatta cattcccagt tgcatcataa gtagtctcgc caggttgccc tctaaaagaa
     1081 taaaatgtta ctactgcaaa gtctttatca aaaacacctt ggcaagcaaa tgtaaaatta
     1141 caataaaaat aaggataaga agttttatga ttttcaaaag tgatgaagat gttaggtgta
     1201 aaatttaatc cggttacttt aacccaacga ttataagcat aacttttacc accactataa
     1261 gtaacctcaa aaagagaacc tctaccctca gcttctaaag cagtagtcgt tccacttgca
     1321 actttatact ttgtatttaa ttgacttata gtattattag cttgtgttaa ctgttttatc
     1381 aaatcttgta aactagcatc tttgctatca aaacctacct taatcttctc tgataactcc
     1441 acaagtgtgt tattcaaact tgcttctata ttctttaatg ctaaagtatt tataatgctt
     1501 gttttaccgc ttctaaatgt atctttaatt tctatccatt tgtttgtaac ttcatctgtt
     1561 gtagaaccag cagggagagg tgcaattcct ttgcttatgc ttagtacttt ttctgctgta
     1621 gcattattac tgtctgtaac aactatttta agtgtgtgta gcgcattatc ttctaatgta
     1681 tagttaattg ttttctctgt tgttaaatct gttgttatag tttcttttaa tgtatcatct
     1741 ataaatactt ctatcttagt tagtagcgta gggtctgtgt ggtcagcttt aaatgttact
     1801 tgtgtagagt tataagagga tatatttaaa aaaggtaatg cttggagtaa tgttatttta
     1861 gcgtagccat ctgctctagt agtattacct ccagtagtca taactacatt ttctagccaa
     1921 tattcagatg ttggtatata tccaggtggc ttataactat ctttagttag tgcgtaacca
     1981 cttcctccgc ctgcaacatt tcccgcattt gtgcctccgc caccatacca accaccacct
     2041 cctcctacgc tataatatcc aataccacct ttaccaaatg aaccatgata ctcgatagtt
     2101 tcttgattac ttccaccttg atattgagaa ccgccaccac taaatctgtt agtgttagca
     2161 ctaactccat cagaaccttt ttcaccacca ccatgaccaa tagaaggatg cggataagaa
     2221 aaaccactaa ttgctccagc tccacctgca acaagtatgc gagaaagtaa tccttcagaa
     2281 ctatcccaag ttgtactaga ataataaagt cttatatcag tagcaccacc tccataccca
     2341 gcgtaaatat aatctccgat atttgttgaa ccagcacgac caccaccatt aaaaccagtt
     2401 ctagtaatat ttgagtcttt tgcaagtttt tcataacccg attgaccaac gcaaacaatt
     2461 aaattagtac gttttttcaa tataatttca ccttttgcat aaccaccctt tgcacattca
     2521 ttaaaagaac ttgcaccttt gccaccacca catgcacccc aacattctag tttatatcgc
     2581 ccaggtagca atgaaatact tcgtacataa ttagcataat cgaaattcca aaccgtctgc
     2641 attataaatc actctccttt ctaaataggt aacatatcat tttgtatgtt tatattaatc
     2701 tcgttaaaat tttctatttt tctaacaact tcatctattg ccccttgcac atttgtagca
     2761 ttaaggttgc ttgtcgcatt attataactt gttttctctg ctgttgtttc tatgctatct
     2821 acgctagttt tcacctcatt gattgcacta actatatttg ttttatctgt tgtagtaagt
     2881 agtgttgtat cacctatttt attatttaac tctgttttag cagtttctat gttgcttgtt
     2941 aattctgcct tagttgtatc aattttagtg tctaggtcct gtatgtcttt taaagttgct
     3001 agcgcaacag ttgggtctat ttttaaattt atacttgctg tgttagacac agctaatata
     3061 attttaatta atatttcctt cactgttcct gaatctggag caggtttata agtttctgga
     3121 tagctagata ttgcgagaag ttggtcttta gagtcaaata ctccaacctc tctgattgta
     3181 aagcttccag tatctccagt tatagtcttt tgtataacta cccaattagg atttttttcg
     3241 tctgcttgaa catgctctaa tgtgctttcc cacactacat ttttgagttc tgtttgagtt
     3301 tctgttggaa tatatgcact tccaccacca tccccaactt ttatttttgc aaaatccact
     3361 ctagttccaa gtgctgtagc gtttgctata gccgctttgc ctatttctgt aagtaatgta
     3421 taatattgtg cttgtgccaa ttatatcacc tcctgtttag ggtataatgt taccctttca
     3481 gcacttctat tgtttccact agctagtatt atttctccaa aacattctat atttttaggt
     3541 gtataagggt agattgtaac cgtttctcct gtactggtca ttgctcctgc ataaagtcca
     3601 ttttgattat atagaatcat ttcaaatttg tgctcaagat gtgcaggttt tatttcttct
     3661 attttcttat ctaattctaa aatagtgtta taactacaat tatttgttat aaagctaagt
     3721 gtaaagctaa atagagtact aaatactttt acgtctacat ccgttttggt atatgcttct
     3781 cctatcactt ttataacctc tatagtagtt gtacccttgc cacgcatttt tgcttttata
     3841 ttacttcttc tatcttctat acttaaatca aatctatttt ttatagataa aatattttcc
     3901 caataatcaa gtccccatgt tgcagtatct acaaaaaatt ggtcgaagat atcgtcataa
     3961 gtttctctaa gtgtttctag ttctatgtca taagcttctt gtattttact agtaatatca
     4021 ttgttgtaaa aagaaggcag tttatcaatt agtttcatta aaccacctca ctaaattcta
     4081 gtgttgtcac acttggtact ttgtcctctt caaatactat attttcagct ttgttattta
     4141 acagtaaatt actaaagtca tgtaaacctt ccacacttgc aagtattgca cttactttag
     4201 tgtaaattat ttctttatta atatttatta aataactatt aatactttct aagaagcttt
     4261 cttttacaaa atctaatgta tatcctactt ctagttttat agttgcactt atgcttatat
     4321 taagtggcga aggtgttgct actgttagtg tagaccctat tggcatttct tcctcgatat
     4381 gctctttgca tctttcaatt acttccgagt caacagcttg attattttct ccaaagatta
     4441 aaacttttac cgttcccgga ccatcccatc ttggataaac cttaacatta tagactcctt
     4501 ctacttccaa agcccattct tcatagtgag ctttatttcc acttgtagct tgatttcttt
     4561 ggattttata aaatcgttct tttagttctt catctgtctc tatatctgta ccaccttcaa
     4621 aacttcttgt gttagtaatc tttgttactc cagatatatt atcaactagt ttaaattcac
     4681 aatttgtagg taaattatat ttcttaccaa cttccagagc ttgaactggg ctattgtcac
     4741 cttcttcact accaatagtt acatctttta ttactacaaa tagtaaatct ctatatgata
     4801 ttactgtgcc attatttatt acagtacctt tctctccaat aaattcaact tctccatttg
     4861 actctgtacc taactttcta tatacaccaa attcattgac tcttttgtcg agaaactggt
     4921 tatatgtgtc ttgtataaaa gccattttat gcatctttga aagttctaga tatatcttcg
     4981 aaagttctag attatttcca gataccatgt tgtttagaaa agaaccttca cccttataaa
     5041 tatcaagatt aatattttca agagttctat tttttattac ttcatatgtc tggtcactat
     5101 acatttattt ccacctcccc atagatagtg gatactttta catttgcact caatacgtct
     5161 ctgttaaagt tagcactttt tacgtttact tctaatatat atggatttat tagaagggcc
     5221 tcttttatga atctactagc ttcactttct gtaagacctt ttgtatattt ttgtcctatt
     5281 agttctgaaa gctctgtccc atattctaaa gagtatatct catgttcata tctatttgtc
     5341 tttatacact tgtatatcca aacttttaag gcttcttttt tttcaattat tttaaagtca
     5401 cctttttcta aaataggttc atctttttca aaatcccatg ccacttcacg aaagattggc
     5461 aattcttctg ttttaggtaa gatataatcc tctgggacac ctataaaagg aaatattgta
     5521 ctcattataa gctcaccaac ttacttacaa cagcaaattt ttcgcctatt ctaaacatta
     5581 ttaccatatc cccagattca aagttatcta taaaaggatt ttttacttca tgctggtgtt
     5641 cttgatttat ttctgtgtca aatgtttcta gctgtctatc aatcatccaa ctatctatca
     5701 aaatatcttc tttttctaat atgatgttat ttgtctctat ttttaaatct ggtaatttac
     5761 ttttaatttt tccaataaaa aaagaaggtt gattatgata ctttccttct tctcttatta
     5821 ttcctataaa ttcatttatt ggattagcca ttatatcacc atccttttat aatactctcc
     5881 tagctgtatt aaaatctctt ctactactta acttacttat tttaactaca tcaccttttt
     5941 gtggagcatg taaaaattct ccattaccta tatataatcc aacatggctg actggattat
     6001 gaaaaaatac taaatctcct gctcgaagat ttttttgttc tactttcttg ccttttttag
     6061 attgttgatt tgacgttctt ggcaaactaa cattaacttt tttaaaacag tagaccatta
     6121 aaccagaaca gtcaaaacta cttggtccat ttccacccca tttataaggt ttacctagat
     6181 gtttttttgc ttctgatatt actattttag ctttatcaga tacattacca gaataaccac
     6241 caataataat ttttcctttt cttcttccaa atttactagc ttcttcaaca tttccaaata
     6301 aaatatctat atggtatgtt ccattctttt ctatagttat tgcaggtcct acatcattta
     6361 ctttatagac tccatcacga ccagaaactc ctgtaattac ctgtatttca tctccatagt
     6421 ttaataaagg atgtttattt aaaaactctt ttgtataata agtttgctca tatttaccaa
     6481 ccataggagc agcgcaagtt tttttagatg ggtcaagttt tttctttcta caatctgtat
     6541 ctccaccttc ttcttttcta ggacagtaag ctgtaaattc tgctgtaaac tcttttcctc
     6601 ctgcataatc ttctccccct aaattacttt cttccttagg ttcatcctgt cctgctgact
     6661 tttcatccat aagattttga aaattaagtt caagctcaat ttgatattct ccattttgcc
     6721 aagtatgttt gtctgtatct atataaaata gtcctacaag ctttgtataa gaatctttaa
     6781 cttttactcc tctaccagtt atacaactta catctccata acctttaaga gaacagcttt
     6841 tttctatccc attaaactcg ctatcaatat ctacatcttg attttcttgt tgctgaatta
     6901 ctttttgcat tattacattt acttccttaa aaatttcatt atctattttt tcgctaatct
     6961 tacttccata ctggtctacc actattactt tattttttac attttccatg ctctccgaaa
     7021 aggtggtatt tataatatta aatccctctt caaacataac acttaatgta acagttcctt
     7081 tttcaataac attaaactta tctaaattgg cctctatcat atactttttc tttgtctttt
     7141 tacttgcctc tgtatatgca ctcattattg tgtcataacc atttactcct ataaacatct
     7201 ttgtatactt gacattggtc tttgctattg ttccaactga aagcctattt tcagcaaata
     7261 cttgctttgc tatgtcttca actaacttat ctttaaaatt gtatgacact tcactttgtg
     7321 taagtaaaaa tcccatatct ttagatacaa aactaatttc attactgctt gaatctttag
     7381 acctattaat tatcattcct ctaaagagtt ctttctcatc tacatagaaa cagactgtac
     7441 tagctattgg tatatcgatt tgttggaaat ttacatcaaa tgatgattgt attattgaaa
     7501 actctagtgt ccttgatgga gatttataat cacctgacca tgataccttg tctactatgt
     7561 cagttatgtc atatatactt ccgtttttta tgtgtaccca aatcttaata ataaaaaatc
     7621 acctgccttt tcacttaatt ctatcagcag ttatttacaa cttattatct ataatagtta
     7681 ttgttttgga gttacttttg gtatttttat tcttttatac tcttttaagt ctaatgtaaa
     7741 atacacatct cgagtcccat ctctttcttc ataactaaac ccttcaatta tgacttccat
     7801 atttatattt gtttccgtaa ttgtaaatct taatataaaa ccttccttca tccatttttc
     7861 tatcttattt acacaatcat atggtgatgg aaaacctgta tagtcacaga aagtgtagtc
     7921 tttatttgga aaaaaacttg atattgaaat gcttttaaga gctacaccac caaaagttgc
     7981 tacttcacct attttgagta tactagaaga gtttacagca gcttttccat taatactaaa
     8041 ggaagatgga aatactggaa atctaaatct atcttctgct tgtctaagcc acatttccat
     8101 tataagacac cccctaactg tgccaattgg atacttgcaa ctaattttga tgttatcttt
     8161 tctatatcag cttcttctct aatcactatt gtatcagcta acttagctat gtttatattt
     8221 ccaccatttt ttctgcttct atattgattt gcttcttgtt tagttaaaac catttcacct
     8281 tcatggagtc tggtttgata gtcattataa ggaacatagt tcaatccaaa tgcattatta
     8341 cctttcttac ttgaataagc tttaccagaa tcaccagcct ttgttccagc atatggatta
     8401 tctttgtaat tttttttact cttttccact agatttaaaa ctgttcctgt gatgcttcca
     8461 ttttttatct tattaatcat agtttgccac catttgcata tatctttaac tattttacat
     8521 actttatcta atatgttaaa aaaagctccc aatattggct ctataatttt ccatccagtt
     8581 tccaacagag gtcctaaggt ctcccataca gttttccaaa taacacccag tgtctctata
     8641 aaattgttta tttgaggtgc atattcacct aaaaaagtaa aaattcttgt gataatagga
     8701 ctaacagctt caactacagg tccgattagt tggaatgctg tagttactat tgtaccaaat
     8761 atacttatta atccttgtgc aattggtgat ttattgttta tactatctag taaattattt
     8821 actaagttta ctactggaag taatgaatct attaatgctg taaaatcgat attttgtaat
     8881 agtgtacttc ctgcgttaac aattgctttt ggtgcattcc ctatcattgt tgctgcatga
     8941 ttttcaaaac gttcttgctt attttcaacc atcattttac gcttttcagc ctcattagaa
     9001 attcgatatt gagtagaatc agaaaactct tttccatttt ttacacttct agattgcatc
     9061 tggtcatatt tgttgccaat aattatctta ccattcatta cattgcctat tttaccagcc
     9121 attttttcag ataagccagc taaattgcct aatccacttt ctataaaagt ttttattttc
     9181 tttagtcctt gacctaaaat atcatcaaaa ggtttaacaa tactcttcaa gccattgtta
     9241 atgcttgttt gagctcctga tattatacct ggtattgtat tactcatttc tttagctaac
     9301 cccatctgct ttacacttat gccttccaat gcttcatcaa aagttttata attttctcct
     9361 accagagtat taagagattc catgtttcct tttgctgcac ttaagaaagc tgctgataca
     9421 tcttgttcac tacttgtatt catatttaaa gctctaacat ctaccatagc ttgagttata
     9481 tctttttctt ttttgccacc agtaatatta ctaatcattt tagctttagt tcccattgca
     9541 acaacatctt cagggtcaaa aggcgtatca tttgccattc ttactaaact gccataataa
     9601 tcatcaacat ctatttgatt aacttttcca gtatttcttt tcattacact ttctagaaca
     9661 actttttgat tttgttcctg accaagtaaa tcaacaccct ttttcacagc gacacctata
     9721 gtagcaccat ttgtcaaatc agatatcttc ccttttatcg aaccaagtat tccacttgcc
     9781 atatccttta cagctattgc accactccat accttacctg caaataatct caatttggga
     9841 aatactttac ttataatgcc acttgccata tcttttatag ctattacacc actccaaaaa
     9901 gtatttttaa acaacattaa cttaggcaaa attctaccca tagcactact caccatatct
     9961 ttaagattaa tacttccttg ataaggacgt ttctctactc gttttaattt tcccaagaca
    10021 ctacttataa ctctcttagt catatctttt gcttttatta cgatattcca aatcttgcca
    10081 ccaatctttt ttaaatttcc cagtaattta tttttagttt tttctacttc atcactttta
    10141 gctttaaata ttaatataat cataatattt gaattaaact catttttctt ttgaatcaac
    10201 ttattaaaat aatcaataat catagctata gttattgcta gtctcataat acttccattt
    10261 aaacttccaa tcactcctga aacagcattt atgctagaac ttaattttgt catagttgaa
    10321 cttacactac tagcagtagt ttgaactttt cttaacgagg aatttaaact gtttactcta
    10381 ctacttgcac ttccaataga attttttatg ctactagcca ttacattagt ggaaagtgaa
    10441 acagccacag ttgctctcct aaaattatca gttactgata taatttgatt catttttggt
    10501 gtgtaattat ctgtaaggta catatttata ccttcttctc tcacatttcc cattacatac
    10561 cacctcctgt caaaggacac actccatatc catgtttcat tttttcaact tcttcctcaa
    10621 tttctaaggc gaaaaaagct tcaataagct ttaattcgcc tttatccatt gcataaaaaa
    10681 gggacggtct tatacctttt ttcttccaat aatagtacat tattgtagtc ctggtatccg
    10741 tccctattag ttttttacct cttctatcac tgcattttta ccataaccca taacatcttg
    10801 aagtattcta tataggttgg taatttcacc tggtaataat aattttcttg ctaattcttt
    10861 tggtgttgga gccttaaatt tatccattag ctccttattt ttaaataaaa gctttccatc
    10921 caaatcacaa acaccttcta taattgtaag catctgcatc tccggtaagt ctatatccgt
    10981 tttcatattt tcatcaattt taagacaagt attttgtatc tcttcatctt tacttattgt
    11041 taaagctcta cacattactt taaattcctg cccaaatata gttgataatc tcttaagttc
    11101 aactatagta cttggtcttt ctaagttttc tgtatctgca tttaaaagta aatctactat
    11161 gtttatattt tttgataatc cattttcact cattattatt tgctccttta tcaatcaaat
    11221 tcaatcaact aactttttta cttattaact atctgaattt attaagtata tatttactaa
    11281 atatcagtat aaaaccaaat ataaaactaa atataaaact aaattatatc aagaaaatca
    11341 tactcagtaa atgtgaaagg tgcttctatc tctcctttta cgccaacctc ccaatcagct
    11401 aaagttaaat catcaaatgc tatattcttt actgctattc tttctgctcc ataagaatct
    11461 gggtcattta atttagatat agcaacaaat ctaggttcag aacctctctt tatcttttct
    11521 ccaattaact tgtgcattct tgaactaaca tggtatagag ttattgaacc ttttccttta
    11581 tatcccatat actttgtatc agtacccatt tgacctgcta ttataatatc ttcttttgta
    11641 aattccatct ttgcttgaaa cttctttact tctgctactt tgtttccatc aagccaaagt
    11701 tctccccaag taccactcat tacatttcta gcttccatat ttgccatatt aataatcctc
    11761 ctatatttct attgataaat ctatatcttc catagcatca agtactttta tttttgcttt
    11821 taaaaatact ttagaacctg tgttagcttc ttttatttct tgtaatgtca tataagataa
    11881 atctactcct tttgatttta aatacgattt ttgtgcttca aaatctattt caacagtaga
    11941 atcagattct ataagtgctg atttttctaa ttcttctaaa taacttttta tagctactat
    12001 caataaacat ttgttgtcat aactgttagt aacctttcct atatagtcat ctattatcac
    12061 ctttcttatg tcactatgta taatatctaa agtgtcaact atttttattt tctggaacat
    12121 ttctcctttt tctgctgtta attcagttaa agaatttact cctctagcaa ttcttatagc
    12181 tcctgcttct ttaataagta taagctctcc tttattaacc cttgattctg catcaacttt
    12241 cgtcatctta ggtatatcga ctacatcact aagtttagta taagttactg attgacttaa
    12301 aggtgtacct gctataagtc cagccaccct acttgtaaac tcatcaacac tgtatttctt
    12361 ttctccaact aacacatctt ctgtagtaaa attaattatc ccttcatgat ttcctacaac
    12421 ttttcctaat acagctttaa ccttaacctt atctatatct ctaagtttaa ttatccaatt
    12481 ttttatagca gtcttatcag cttctactgc ttttggcata catagataat taaattcctt
    12541 agtctctaaa aaatctaatg cagtttgaat atctgcttct ccttctacta cataaactaa
    12601 taatttattt ggagtgttaa catttcccat caaagctaaa tttatatatt ctttattttc
    12661 agcagataaa tcaactggta tatcctcttt ttcatttatt tcattaagac ctagtgcctt
    12721 agcatcttta agcaccattg caattattcc tctagctgaa cgttctttaa cagttgtagc
    12781 tagctcctta aatgatatgt tgatacttgg taatcctata gccatactat cactctccta
    12841 aatttaaatt gatttcttcc atattttcat atactacatc ttcttcatat acactatcca
    12901 gatagtttag tgtaagtgta aagttcaata cataaattga ctcttctttt ttaatacttt
    12961 gttctagctt tgttatattt aattttctgt ctgttacctt tatatttctg ttaaatagct
    13021 tatttaattc atctgaaact tcatacattc taatactctt tgactgtggt tttggtaagt
    13081 attttataga gataacaata tctttattat tcgtatttaa cattacaggt gtacaaataa
    13141 ctgatagtat acttacaaaa aaacaagacc cttcagagcc ttgcacactt tcatcatcaa
    13201 taaaaatact ttcagtaaag ttttttttga gaatctcaat aattgtttct aatatttcct
    13261 tgtatttaag caaataatca ccacactatc actaaatgtt cttgcttaat ctgataatct
    13321 tactataaat gatataagtc atttttaaat atatatctca tttattcctg tcaagtatct
    13381 tctatatttt taggtaatat tattcttttc aatctatcac ctccttcaat atggatttct
    13441 ttcacaataa cattatctca catattttat tgcctattgt tgcaatggtg tatataaagt
    13501 gttgtaaaat tgtcctattt tttataatat ctcactcaat ttaaatatca tctttttctt
    13561 taaattgtag caagatgttc tatctaagtg tagtttcata gaaatatatg tcatattgtt
    13621 ttttgtctta ctattataaa aaagattaaa aaattccatt tctatatcat ttagacacgt
    13681 tagagcattt tctatcttct ctttttctat ttctatatct tctttttcca tttttaattt
    13741 tcttaatctt tcctctcttt ttaacacctc attttccaca gaagagctta tgttatatgt
    13801 gttactagtc ttttctgtat ataccattgc tccacatcca gagtattcat tctcacatct
    13861 ttttatgtcc aagttaatat tctttatctt tatatccaaa tacttataat tatgtaatct
    13921 tttatctgtt tctttaaata atatatcttt tttgttttcc atagtacttc ccctttttta
    13981 tatacttatt attagcttca aatttacaat aatctcttta gtgcacagtt ttttactaat
    14041 ctcacaacct tcaagaacat attgttctat aattgtataa aaaaaacttc ttctatattt
    14101 tgttctaaaa tactagaaat cttcttagcc ataagaagtc caggattttt tatgcctctt
    14161 tctatttgag atatataatc atttgtaatt ccagcctttc tggcaagttg aatttgtgtc
    14221 atacctttgc attttctata atatttcaag ttatttttaa acattgtttt atacctccat
    14281 tactattaag aatatttcgt tcttcctcac ttagattata tagaattatt tgttctttat
    14341 caatagattt tagaaaaaaa tattctttta tattttatta gttttatttt ttagaacaat
    14401 ttgttataat ctatacataa ggtggtgtta taatgtttgc caaaagatta agagaactta
    14461 gaaaggaatt tggattgact caaagagaac ttggagaaaa agtaggcgtt tcccaaagag
    14521 tcttagggta ctatgaaaca gaaaacagat ttcctgatga acacatttta aataaattgg
    14581 ctgatgtatt taatgtatca gttgattacc ttcttggaag aaccttagtt aaggaaaata
    14641 ttgatacagt agctgcacat agaaaaaatc cacatgaaga gttacctgaa gaagcacaag
    14701 aacaacttaa tgattatata gaatttttac taaataaata taaaaaaaaa taaaccatat
    14761 gatgagcagt ctatttttct gctcttttaa tataatttaa gggcaaacat acattctatt
    14821 ttagggggat ttttatgaat aatttggata agctttttga attagcttct caagaagaga
    14881 taataattca ttataccact tatattgcag gtgatttaga aggactatat ataaacaaac
    14941 atggtattaa aattatatca ttactcagta atttaaaaca aaactcaaaa aaattgacat
    15001 ccatcttagc tgaagaactt ggacatcatt tcactagcct tggatactat gtatcctctt
    15061 ataacgatta ttatacaaaa attattatag ataagtgcga aaataaagcc ttaaaatggg
    15121 cttgtgagtt cttaattact gaagaagata taataaacat aattaactct ggcatcactt
    15181 gcgtttatga aatggcagac atacttaatg tggatattac tttctttcaa aaaagattag
    15241 aatttttatc attaaaaaaa cagtctttac aacttggaaa taataaatat ttaatattaa
    15301 ctaatttgcc gtatttctac atatttgatc caatttcata ataaaaatag tagtaatttt
    15361 aactggctta acagtaaaat ctatatatt
//
LOCUS       DAELJO010000080        15318 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELJO010000080 DAELJO010000000
VERSION     DAELJO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.080"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(62..349)
                     /locus_tag="KPB88_002111"
     CDS             complement(62..349)
                     /locus_tag="KPB88_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054387.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(369..644)
                     /locus_tag="KPB88_002112"
     CDS             complement(369..644)
                     /locus_tag="KPB88_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054388.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(1281..1460)
                     /locus_tag="KPB88_002113"
     CDS             complement(1281..1460)
                     /locus_tag="KPB88_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054389.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1745..3211)
                     /locus_tag="KPB88_002114"
     CDS             complement(1745..3211)
                     /locus_tag="KPB88_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0054390.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            3806..4984
                     /locus_tag="KPB88_002115"
     CDS             3806..4984
                     /locus_tag="KPB88_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0054391.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLIFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIIPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            5334..6215
                     /locus_tag="KPB88_002116"
     CDS             5334..6215
                     /locus_tag="KPB88_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0054392.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            6424..6573
                     /locus_tag="KPB88_002117"
     CDS             6424..6573
                     /locus_tag="KPB88_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0054393.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(6833..9400)
                     /locus_tag="KPB88_002118"
     CDS             complement(6833..9400)
                     /locus_tag="KPB88_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0054394.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSISDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(9403..10008)
                     /locus_tag="KPB88_002119"
     CDS             complement(9403..10008)
                     /locus_tag="KPB88_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054395.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMHDGGE"
     gene            complement(10382..11842)
                     /locus_tag="KPB88_002120"
     CDS             complement(10382..11842)
                     /locus_tag="KPB88_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0054396.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            12175..12753
                     /locus_tag="KPB88_002121"
     CDS             12175..12753
                     /locus_tag="KPB88_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054397.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            12804..13037
                     /locus_tag="KPB88_002122"
     CDS             12804..13037
                     /locus_tag="KPB88_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054398.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            13046..14296
                     /locus_tag="KPB88_002123"
     CDS             13046..14296
                     /locus_tag="KPB88_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0054399.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLRG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            14457..15026
                     /locus_tag="KPB88_002124"
     CDS             14457..15026
                     /locus_tag="KPB88_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0054400.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         5318 a         2500 c         2013 g         5487 t
ORIGIN      
        1 cctttagttt aaataattca cctataccta cttgaatagt tcacttttat ttgcttaaat
       61 tctataccaa tactggctta aacattatac taccatgatt gtattgtcta tcttcaacta
      121 caataaaaac aggttctaaa tctttagtag atgttatttt taattctaaa aaaacattat
      181 cagttggtaa tatttttaca tcataattat ttaaattatt attcgtgata ctattaaata
      241 catcaccttg acgttcaaaa aaaacattat caactattac aaaataaaca tcttcttttc
      301 tactattttc aacctctata gtattttgtt tagttacatt tagtctcatc ttccacctcc
      361 tattaatatt acttaattat attaatactt gtaattgttg cattatgttc atcacaagga
      421 agaggtttaa aaacaactat ctctgctaaa taattctgtg gaggatttgt aactgaagta
      481 gcattgcaaa taaatttaaa atttgttcct gctacaactt gagtagaaac aataagtggt
      541 tcataaccaa ccccttctaa cattcctaca gcttcattaa atacttttaa atcttcttct
      601 tcgacagctc tgaattttga ccatccacca acaaaaagac tcatataaaa acctccttta
      661 tatatatatt tatttatata tatagtagta ccaattttct caatattcaa tattttctgt
      721 gtaactggtc aatgaaaatg cataactgga taaaaatact tataaaataa cttaacttta
      781 catataaata gcttacttta catgtaataa gattattcta caatatgaaa tgcatcttgt
      841 tttgaatata ttctaaatac aataatggat attttcaatt attataatta gaaaatatcc
      901 attacttaac ttgttttatt taaaatcaca tagagacttt attatttttt ttattgcatg
      961 aatagctatt tcaggtatca taaggtgata taccccctcc ttgaaaccac aaaaaacatg
     1021 acaaacttta cgaatcttgg cttaaaagac cacaggtaca ttccttattt gtggattgga
     1081 gtagccataa caagcaaatt ttacaatttt tatgatgaca aaaatcaata aaatcgctta
     1141 gtatataaca cgaaacacta tttgttacta ttaagaatag ttagatagga agtatgcatt
     1201 atacaaataa aggaatcaat aaagaaagtt aaaacatccc tacaaattaa aaatctgtta
     1261 catttttcta tatttaaacg ttatttactt acaattggag aaataacagg ttttccttct
     1321 cggtcaagta aaggcgtaaa tccggctgca ttgccataat agtgaaatac atagttcaca
     1381 cctgtttctt tatccaccca aatctccgtt gcatttattt ttccttgtga atatactttt
     1441 tcaaatctat cttttcccat tttataatcc cccatatttt tgattggtct atgccttctt
     1501 cacttaagta gaaaaaatac ataacttatt tttttaattc taatatgagc acgtaaaaat
     1561 gtcaaacagc ttatttaatt tcataacttc atgttactgt tcgtaacctt gtttgtttac
     1621 ttacactaca ttacagcacc attttaccct ttacatgatt gtattgcaca cagcaaaaat
     1681 gcttacttta ttggattttt caactaaaaa aaggattttt tctccacaga acatttacta
     1741 ttttttataa tatatctaat tttactctca tatcaacatt taatgaagat ataacctttt
     1801 tcaaatattg taaagactcc ttaaaaatat tagaaaaaga ttctgcaaat ctctcaacat
     1861 ctcctttttt taaataccta agtatctcaa aacatacatc aaaacgtgtt ttcaaaattt
     1921 cattatattc ttcatgaaaa aatctctcta aatagattcc aaatatcata tattgtttta
     1981 aatgcaaaaa tgtttcctgt aatacataaa tttgtgtata atctactatc ttacaaagta
     2041 aaacaaaatc tggataaacc ttacactctt cacaattatt ttgtaagtac cttaaatgag
     2101 tttctatttt atctacagct atcaaatcca ttttatttcc tagtaaaagt actacatcct
     2161 caagagtaat cgtaacaaac tccatagcat ccaaaagcaa actcaacctt ttagctcctt
     2221 cttctgtatt tatataatca tcatcatatt cagaaaaaag tgttacatat gttcccttgc
     2281 cattaattgt tttagcaatt cccaattcat ttagctcatt ataggcactt ctaactgttg
     2341 gaagtgatac attatatcta ttaattatcg aagtaattga tggtagacaa tctcctattt
     2401 taatattgcc ttcaaaaatt tgacaaatta catctgaaac aattaagtca tataaataac
     2461 atttttccaa cgttacagaa ttaaaactac ttgtatctat actttgtgga gcacgatttt
     2521 ctaccatttt aagtatggat tctctacaag ctttatacat atatgttaat agttttttaa
     2581 attcaattag attcccgtcc aatccatata agtaagtttt tcttacctgc tctatgtatt
     2641 tattacgaaa aaagtctata caacaattat ccaagttatc gaaattatag gccaattgtg
     2701 aaataatgac atcatgctga ataatctcac aaaaatggtg aatcaggaaa ttatttgttt
     2761 tacttattag tttatttata tattttagcc tttgttcttt aaagactaaa taattagaac
     2821 tatttttttc catacaatcg ataatctcgt ttaattcttc aaagtcttta gatgtaaatc
     2881 ttttaatatt gtacatagca atagaaggaa atattaaact aaaaatctta tatatttctg
     2941 atatttgtaa aatcttctca tataataagt cagattctat tgattgttta ctcgtactct
     3001 ggattaaaac tggtctagaa ccagcttttg tctctataaa gcccttttct gagagcattt
     3061 ttattacagt ttttatagta ttttttgata ccatatattc ttctgacatg ctttttatag
     3121 aatcaagcct atctccaata gtaattttac cagtataaat ttgttgaatt atatgctcaa
     3181 aaataattaa atattgtggt tttctcttca tatttttcct cttcgataaa ttagatttat
     3241 tgttttttgt aaggtatatt tacttttatt taatagggca aaatagttgc ttattttttc
     3301 ctcataatct ataattaata taaaccattt atatatatgg tctaagtttt ataatactgt
     3361 aatgcagtaa aatagcatat tgtagctata ctacagcaaa ttaacatacc attgtagtat
     3421 attaattgga ataattttaa gtacttcaaa attccagcaa aacatatatg tgtttttgat
     3481 attatgtata ttcgttaata gaatagctat acttgtttta cataataaat tatatgatac
     3541 ttgctatatg taattactat atttaataca cataactatt atactttcca tacataatta
     3601 ctatacttgg acacatagtt atcatacata ctgtatatta agatataata ctttttaaat
     3661 tttatataac acagacacat gcaataaaaa agaaggtatc cataataagc tctttgcatg
     3721 tacctaaata aaaactgtaa aatagaatat ataaaatcta atctatttta agtaaataca
     3781 atttcacttt aaaagaggat gtactatgaa ataccatata tttttaatca taactactat
     3841 attaataatc gaactatgta acatttacat atgcaaaata gcatcttttg gaaacaaaaa
     3901 tataactata cataagaatt acaaatatat ttttttaata aaaccattaa tcttcttgat
     3961 aataatattc acaaaattac cattaccact atattatata ttgttttaca tagtaagtca
     4021 taaattgctc ttaagcagaa aaagttctac taacaaccat tttattccca atatggcagt
     4081 aataagtttt tgtattttac accttattac tgtaagtaca atatcacttg ttaaaaatac
     4141 aagtccagat ataatatttg caaatatgac ctataagtta attagtattt taatagcttc
     4201 tattttagat tttatcttat ggtactctat gaataaaata aaagagcata tttatataat
     4261 aactcaaaat caaatagagt tcagtctatt tttaaaattt atctggttca ctttaggata
     4321 tatattattt gatagtatac aaattacatt tgctccacca agttctctaa cttcctactt
     4381 tatattggga agtaacatat tattaatctt acaactaatt atattcatca atcatatata
     4441 ttccattgaa aaaaatattt atttagaaaa agaacataat agactaaaaa tgataaaaca
     4501 agaacaatta ctaaaagtaa atttattaaa gaaaaaagcc aacattgatg aattaacatc
     4561 agcatataat cgaaaatttg cttttgaaaa aatacagcat tacattgata acaatttatt
     4621 attttcattg atatatatcg atttggataa attaaagacg ataaatgata catatggaca
     4681 tttagctggg gataactata ttattaactt ctcaagtata gttattgaaa acttaggaat
     4741 agatgacata tttgctagaa taggaggaga tgagtttata gttattatac ctaaatctaa
     4801 tgaaaacacc acaaaagaat ttatagagaa aataagtaaa attatggata atacgatatc
     4861 ttttagctat ggtattgtag aagtttctag cgaaaacaaa atgactgtag aagaaattat
     4921 taatattgct gatattagaa tgtacactaa taaacaaaat aatcataaaa ggaacaataa
     4981 ctaaaatgaa tatttacatg agcttacttg tagtctcatt atgtttttta tcttattatc
     5041 tctatcatta taattttttt aaatatctgg acataatgat taataactct aaaaataaat
     5101 acacagtata tattatatat tctatattta attatttaat ttttattatt tgtacttatt
     5161 taaagtttca tctaattata aattggacaa tttttatgtt gattttattc atacaaatta
     5221 aagtaactta taaaatctcc acaataaaaa acatttttta ttcactaaac tgctccatca
     5281 ttggtttagc aataaatata ttttttaggt ctttaatgtc aatagtgtta aatatgccag
     5341 catatttatt tgatagtaat tctattgcaa atatttacag agtatttcct atatcattta
     5401 gctttatagt tacaggacta tatttcagtt ataaaaataa taaaaataat aagattaact
     5461 ttgaggcagt tttagaggac aaaaaaaact taattttctt attgagcctt atgtttggaa
     5521 tgtacttgta tctatgtttg agtttgtcaa tatactatac tgttggaaat gtattgattt
     5581 taaaattata tggaatcaag tctgccataa tagttttggt aggtaactat attgcagaaa
     5641 gatatagctt tagaattact caaataaaag gttatgatat taaaaatcaa atagaatatg
     5701 tagaaagtgt aaagacagaa tatgagatta ataaattgaa tgatattgta tatacagatt
     5761 tactgacagg tttctacaat agaccttttg tgaatcaaaa actatcagaa tggacatcaa
     5821 atcactacaa atttacctta tgttttactg atttaaatag cttaaaacct gtaaatgata
     5881 aatttggaca ccaatttggt gataaataca tatccactac tgctcaaata ataaaaaaac
     5941 ttataaatgt agaaaattcc ttactattta gatatggtgg agatgaattt ttattccttt
     6001 tacagaatac aagttgtgta gaagcagaaa agataatgat taatataaat tatgaattat
     6061 caaaattaag taatactgac gagtatcctt atgctctttc cataagctat ggattagttg
     6121 agtgggatgg aatatccgat attgaaactt taatatcaaa ggcagattct ttgatgtatg
     6181 aaaataagct taaaaataaa tctactttaa actagctagt taagatattc tacaaaatat
     6241 atgttataag agagtactaa gaatgtcaaa atatatagat tttgcaaata ttgaatacag
     6301 tttactaatg aatgcactgg atataagtgt tagtaaacac ctattagatg aacactttac
     6361 tgttgtttgg gctaatgacc gttattatga aatgttcggc tatacctagc taaaaagtta
     6421 aaaatgacca ctgtagccga gggaatagag accagttttc aaaaagattt cttgcaagaa
     6481 atagactgtg atatgttaca aggatacgtg ttctctagac ctttaccaat aaaagacttt
     6541 gaaaaactta tgtttcaaaa ctcctccaat taacaatata aaattgctta ttataagtga
     6601 tattattgta tcaacaaata aaggaattgt ctcaaaattg attttttcta atcaaatttt
     6661 tattttgaga cattctcatt tttttgcata agaaaatacc caccaagcta cggtttttct
     6721 taccgctgtt cgatgggtat tagtagtatt atgaaatctg aaaactttaa gtattactac
     6781 ttccgctagc gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct ttttactcat
     6841 caaacgtcac cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc ttaatagttc
     6901 gtgtggcaat gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg acaatgcttg
     6961 aatggctaag aggaccacca atgtccgtta caatcgcact tgcagatgaa aataatggtg
     7021 tccatgctgg agttgtggta actgcaacca aaacacttcc aggttgaaag ctttcaaaat
     7081 ctgctggact attcagtaca caagctggag ccgtcacaac accagtactg gtaccaatgc
     7141 ccgtcaacac aattttccca tctttctgct tctgttgtgc ttgtgatata gtttttttgt
     7201 ttttctctgg caacttggat ggtgacacat aacccatgta ttttttaagt tcagcttttc
     7261 gaacaggtat tatcccgttc atatcagata tagacttgtt tttatccaac tgttctataa
     7321 gtacctccaa ttccgatttt gtgagccaat aaatatcatc taaatgtgaa atagctcctc
     7381 ctcgtatgaa tcgttctgag atttcctgaa acatacaacg aatcagagga tgtcccattc
     7441 ccatcaaata aatagcattt tcacgcatag gagaggtttc ctgtgcccaa tggagcaact
     7501 taaaaaatag ttttttacgc aatccaccta tgtgttgtaa aatctcttct tctgcatgtt
     7561 ttctacgctt ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt ccttccacaa
     7621 aggtttttac ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat
     7681 caaactcata agcggtacga ccaaactctt taaagtactt attgattcga tttttccatt
     7741 ctatccatac ttcctgtgaa actccaacag gcacaactga agacatgaaa tcttctgcta
     7801 tctttattgc aggactgttt tgcagataaa ggttgatgct gttattttgc tttacccatt
     7861 ctgaaatacc ccacaactcc ttttccgatt gcagaacaat cgtatcaaac ccaagcagga
     7921 aaacggaagt atccgttata ccagcacgtc gaactgctcc ttgaaataat tttgtaaata
     7981 atgtttcact gatagaagca gcaggcaatg taagctgaat ccttgtgaaa taaatacatg
     8041 cggcataaaa tattgtctga ataccctcca taatttgatg ggcgttcata gtatgcaggg
     8101 gcttttcttc ccattgttta atagcatcct catatccttt tcgtgccgtt tcccagcgtg
     8161 caacgctgtt cataagtgtc ctacgtaaag agcgtgggga aagggatttg atagcaatca
     8221 aaagtggctt tgatttagca gaaagataaa catatccatt aataaccgta tatgccccgt
     8281 tttttggtaa taattttttc gcacttttac caaacatatc tatccataaa agctctgatg
     8341 cacggtttac gatttcaaga ccaagcgtag cgaacaaagg tgtaacagga tttggcaagt
     8401 gttctgccaa acttcccttt gtgtatacaa catcctgttc tggcaatacc cattccggtg
     8461 gcagaacagt aattggacga gcctgaacaa tgtacaattt atctttttcc agtgcccact
     8521 ctacatccat aggcatttga taatacttct caattttctt tccaagctgt atcagttgca
     8581 ttacctgatt acgagtaaga gcatattttt ttctcaacct ttcaggaacc atggtttctt
     8641 ctgttccttc tgaagtacga accgtcataa tctctttatt tgccacttca tacgatataa
     8701 ttcgttcgga atccttatcc accacgattg tatcgggtgt aactaagcta gaaaccaccg
     8761 cttcaccaag cccccatgca gcgttaataa tcatttcgct acgtctacca ttgattgggt
     8821 ttaacgtaaa cataatacca gatgaatcgg aaaatgcaag cttttgtact acaacagcaa
     8881 gtgcgacaat ctcgtgcttt atattattct tcacacgata agcaatagca cgagctgtcc
     8941 ataaggaagc ccagcatctc tttacagagt caataacttt atcttcacct tgtatgttaa
     9001 gataagtttc ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg
     9061 aacggacagc caccgatatg ttccctaatc cagcataagc cattttaatt gcgtccgaca
     9121 cttcctgtgg catttctcca ttatggaaaa gctcccctat tttcttagat acatcttcaa
     9181 gttggctggt attattggaa tcaataccat ctagcagttt attaattcga gactgaatat
     9241 ggttcgtctc aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg tctggcacag
     9301 ggatgcctgc tgtcaatagt tttgataaag acatcccttt tccaccaaca atttcaatgg
     9361 ttgcttgttt atgttccagt ggcaagacat aataaatcat aatcattcac ctccatcatg
     9421 cataattccg tgcagaaata tattcattgt ttcatccaac attttttccg aactatccaa
     9481 tggtcgtgac gtgtagctga ttgataacat ggagttaatt aacaaacgtg tcacaagagc
     9541 ggtatccgtt ttgcgaaaga tgcctgtggt cacaccatta tcaattaccg attttataat
     9601 atcttgatat gcataacgag catctttaag tctctttcga tactcttctt ctaaataatc
     9661 agattctgta tttaaccaca tcagtacagt tctatactgc tctggaaatc taagatgatt
     9721 aagcatgatt tttctaaggc actgttgtgg tgaaggttca ccatcaataa tcctatgaac
     9781 ttgttggata gttttttcta tttctttctc aactgcatat acaacaatct cgtctttcgt
     9841 tttgaaaaaa tcgtatagac ttgactttcc catgttggct aaaactgcaa tttctctcat
     9901 tgatgttttt tggaatccat tttcagccat gagactcaaa gcaacagcaa ctattttttc
     9961 tcggcgttct gcttgttgtt cgggtgtaag cacaattcct tttggcatag ggcgatacct
    10021 ccttttagcg accactggtc gtttcttttt tatttatcat acagcgacca acggtcactt
    10081 gtcaaggtta atttttttca ttgtatatgg tactcgagat aaaaatgttc attttagaaa
    10141 ttgctctatt taagtagaaa taggcttcat cattagaaag caagactata aatttgagat
    10201 atttatcatg ttcaaatttc atcaatatca aagtttaaga tattaataaa aaaagaatta
    10261 ctaagaaata acatataaag attcaaaaaa ggagttgaac ttaaaattca attacaaaaa
    10321 atcaaattta agacaactcc tttattcttt atctctacta ttctttatct ctatttattt
    10381 attacaactc cctaacattt accactactg ccttaggtct agtcatagcc tcaattgaat
    10441 acttaacacc ttgagttccc ataccagatg cctttacacc taaaaatggg aagtggtctg
    10501 gacctctttc agtcttatta tttatttgaa cagtaccaac ttcaagttta tcagctatat
    10561 aaaatgcctt atcaatatca cttgtaaata ctgatgattg aagcccatac tctgattgat
    10621 tagcaatttc aattgcttga tttatatctt taactcttat aattggaagt actggaccaa
    10681 atggttcttc ccaagcaatt ttcatatcaa catttacatt atctaacaat gttggataga
    10741 ataaattatt ttttctaaca ttgcctgtta ttaactttgc tcctttatgt aatgcatcat
    10801 caaccaaccc ttgaacaaaa tcagttgctt tattatctat aagaggagtg ataactactt
    10861 catccattgg atttccaatt gtcaacttct ctaccaatgg ctttattttt ccgactaatt
    10921 tatccgccac actctcttgt actaaaattc tctttactgc cgtacatctt tgtcctgaat
    10981 atgaatacgc tccagatact atattcttag ctgcaaaatc taagtctgca tcttctaata
    11041 ctatagccgc atcttttcca ccaagctcca ataataatgg taccattcca gatattttag
    11101 atatatgctg accaacttca gtactaccag taaagttaat aaaattaatt cctttatgcg
    11161 ttacaacata atcccctatc tcactacccc tacctgtaat agtattaagt actccatctg
    11221 gtatcccagc ttttctcata atttctacca catgaagtgc gctaatagcc ccttgtgtag
    11281 caggttttaa tacgactgca tttccaccaa taagtgctgg agcaatcttt gacattgata
    11341 aatttacagg ataattaaaa ggagatatag ctaaaacagt acctaatgga acccttgaaa
    11401 cataagacat cttatttctt gttccaccag ggaaattatc tccactaata gcttcaccct
    11461 ccatattttt tcccacatca gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg
    11521 cccttgcaga ttttatgtct ttggctattt caagtaccag tatattagct atctcatcta
    11581 tattttctaa caataaatca gcagccttat gaaatatatt ggctctctta tatacaggca
    11641 tttcagccca tacttttata ctctcttttg tgttttttat aacctcatct acctcatgtt
    11701 ttgtcataga ttgtactttc ccaacaagag aattatctat tggtgagtaa atttctattg
    11761 gctttctcga attagattct acccattttc catttattaa atttttatat acattttctt
    11821 ttgttttaag ttcattaaac atatttttat tcctctctct gatataactt tgtgtattta
    11881 aattatagcc atttattcgt tatgtaaaca tttctttttt tcataaacgt ttacctataa
    11941 aaaaatttta agttaacgtt tttctacatt cttctatttt aataacatac tcgctccaaa
    12001 tctatatagg tcacacttta atactaatat accaatatac taatatatca atataccaat
    12061 attataaaca taaatcactt gacaaattta taagtagaca atattatata tctatgactc
    12121 aaaatatttt taaagtcata gatatcatat ataggataga aaggattgat aaacatggct
    12181 aaaagtttta cagaaataga aaaagacaat ataagaaaaa gactaatttc agaatgtgaa
    12241 ctcaactggt ctaaatatgg atacaaaaaa actaatattg atgaactttg ttcaaaatca
    12301 ggaattgcaa agggttcttt ctatacattt tttgattcaa aagaaaagtt attttttgaa
    12361 gtattaaatg gaatccaaaa cagacttata tcatcagtag atgaaatttt aagtaacatg
    12421 cctcaaaaag aaggttttgc aaaagcctta aagatgttgt atcgattata tgataaaaac
    12481 ccattcttat attcaccaaa taatactgat tacatcactt tattgaataa gatacctaaa
    12541 gagatgcttt cacaacttga atacagcaat gtacaaagtt tttatagctt aattgaaaga
    12601 cataatctca agttaaaaat agagaaagaa aaagcctttg gtgtttgcaa tgccctactg
    12661 tcaagcctat tgctaaaaga agatattgga tatgactaca ttgaagtatt tgattttatg
    12721 gtagacaatc ttgtagaaca catacttgag taataattta aaataaattt tctaaacatt
    12781 tctttataac taggaggagt aatatgattg aattaataca aagactaaat gatattattt
    12841 acaaaataaa tgataaacta aatatccaca caaaacttgt agaccctagc agtgagctca
    12901 tgccacttta ctctatgtta tctataatcg tttctatatt tctaataata ttaggtatta
    12961 aagtaagaaa aaaattcctt ataataattg ctattatatc aattattata aatattttaa
    13021 taatcttaga taggtaggta catatatggt aaaatcctcg aacactttta ataaaaactt
    13081 caacttgatg atattaggtc aaataatctc attgtttggt gcatcaatac ttaaatttgc
    13141 tttatcttta tacatacttg atataactgg taaagcagaa atatttgcta ctatactagc
    13201 agtatcttca atcccaatta tagtattctc ccctattggt ggagctattg cagatatatt
    13261 caacagaaga aacttaatgg ttatttttga tttttcaagt agtcttacag tactaatctt
    13321 agcagtattc ttattcaaca ataatggttc aatattactt gtaggaatta ttatgaccat
    13381 attatcagtc ataagcacaa tgtatcaacc aactgttcag tcaagtacac cccttttagt
    13441 tgataatgag catttaatga atgcaaatgg tattgtagct ggagttgcat ctcttacaaa
    13501 tattgcagga cctgtattaa gaggtgtctt gtatggtgtg attggaatta atgcaattat
    13561 agttataagt tgtgtaagtt tctttctatc tgcaattatg gagatattta tacagattcc
    13621 ttttatcaaa caagagcaaa acagcaacat tataaaaact atatttactg atataaagga
    13681 tggtattgtt catatatgta aagaaaatag atttatacta aaaatacttt ttttagctat
    13741 agcaataaac ttatttttaa gctctatgtt aatagttggt atcccctata tagtaaaaat
    13801 cactatggga gctagtaact tcttatatgg tctagcagaa ggagcaattt cttttagttc
    13861 actggtagct gctgttagta ttggtatttt ctcaaaacat ctaaaaatca ctaatttgca
    13921 tatttccttt attatatgtg cagttatgtt cataccaatg gcggtttcag tatatccatt
    13981 tatattaaac acaggatttc cagttccatt tttaatcttt atagtatcat ctatgctaat
    14041 attttttaca acgactctta tctcaatata tgttataaca gtcacacaat taaaaactcc
    14101 aaatgaactc ttaggaaaag taatgtcaat tctttttgca ggaacagcaa ttgcaatgcc
    14161 ccttggtcag gttatgtatg gtaaactttt tgatttgtta tcagatagag tgtacatcat
    14221 tatactagga gttggattta ttacattact tatagcattt gtatcaaaga taacattgag
    14281 gaacgaaaat ctttagatac aaagaattta gatataaaga tttaattttc actaatttct
    14341 gaatttaaat gcaaaattgt gtatcagtat gttgacgttt tagaaattga ttgatataat
    14401 caatggaaaa taaactttat aaaaattcaa aatattcagc tagaggagtg ataaaaatga
    14461 gttacatgcc aactttagaa caagcatggg aaatattaag aaaatacaat aaggaagaat
    14521 ttcatataag acatgctcaa attgtatctg gagttatgag atactatgca aaggagtacg
    14581 accctgaaag agtagaattt tgggaaatag ttggactttt acatgattta gattttgaaa
    14641 tgtatcctga tgaacattgt gttaaacaaa gagaattaat gacagagctt gatttagata
    14701 aatcaataat cgattctaca ataagtcatg gatatggatt aactggttca gatgttaaac
    14761 cagagcaatt tatggagaaa gtattatttg ctgtggatga acttactggt ttaattggtg
    14821 ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt agaattaaaa tctgttaaga
    14881 agaaatttaa agataaatca tttgctgctg gctgttctcg agacgttata agaagtggtg
    14941 cagaaatgct tggatgggaa cttgataagc ttatccaaac aactatagaa gctatgagaa
    15001 gcttgattcc agagatggca atatagctcc tccatacata taccatagaa gttacatctt
    15061 taaagagcat ttctcttttt gatgcaactt ctttttatat tttgtagaat aaaaagttta
    15121 atattttaaa ctcactatat aataaataat ataatgtata atagtcataa actttctaac
    15181 cttaaaagtt tctaataatc aataaacaat tctaatatac aaatgtcatt atatgattta
    15241 attatatatc ttcaatttat atattaaaat aaatatatat atattaaatt cttatacacc
    15301 aaatttttac attaaact
//
LOCUS       DAELJO010000081        15314 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELJO010000081 DAELJO010000000
VERSION     DAELJO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.081"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            286..864
                     /locus_tag="KPB88_002125"
     CDS             286..864
                     /locus_tag="KPB88_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0054401.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            903..1481
                     /locus_tag="KPB88_002126"
     CDS             903..1481
                     /locus_tag="KPB88_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0054402.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1567..2196
                     /locus_tag="KPB88_002127"
     CDS             1567..2196
                     /locus_tag="KPB88_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0054403.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2186..4945
                     /locus_tag="KPB88_002128"
     CDS             2186..4945
                     /locus_tag="KPB88_002128"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0054404.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5032..6594
                     /locus_tag="KPB88_002129"
     CDS             5032..6594
                     /locus_tag="KPB88_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG0054405.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNINILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6595..7731
                     /locus_tag="KPB88_002130"
     CDS             6595..7731
                     /locus_tag="KPB88_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0054406.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7795..8871
                     /locus_tag="KPB88_002131"
     CDS             7795..8871
                     /locus_tag="KPB88_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG0054407.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8849..10087
                     /locus_tag="KPB88_002132"
     CDS             8849..10087
                     /locus_tag="KPB88_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG0054408.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRIEQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENKYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10141..11229
                     /locus_tag="KPB88_002133"
     CDS             10141..11229
                     /locus_tag="KPB88_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG0054409.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11257..12048
                     /locus_tag="KPB88_002134"
     CDS             11257..12048
                     /locus_tag="KPB88_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG0054410.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12163..12717)
                     /locus_tag="KPB88_002135"
     CDS             complement(12163..12717)
                     /locus_tag="KPB88_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0054411.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12913..14115
                     /locus_tag="KPB88_002136"
     CDS             12913..14115
                     /locus_tag="KPB88_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG0054412.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14191..15171
                     /locus_tag="KPB88_002137"
     CDS             14191..15171
                     /locus_tag="KPB88_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0054413.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT         6047 a         1577 c         2753 g         4937 t
ORIGIN      
        1 acttaaaaca atatagtaca ataattttgt agtttcaaaa taaaatatgt acttgtttat
       61 ataaatgata tataattaca tattaaacat agcttttata catttaataa agtatgtaaa
      121 atctatgtaa aaagtaagaa atataaacat ttttttaatt atattttaaa aatatcaagc
      181 aagttttctt cacaaatgaa taaaaaatgt tataatatga ttaaagaaaa agataaataa
      241 ttagcaagat ttgtttagct aaaattaaca agggagcgat aaattatggg tataacatta
      301 tcaaaaggcc aaaaggttga tttaactaaa ggaaatccag gtcttaaaaa tattttagta
      361 ggtttaggat gggatactaa caagtatgat ggtggatttg actttgattt agatacagca
      421 gcatttttaa caggagcaag tggaagtgtt actaatgatg gagattttgt attttacaac
      481 aatttaaaac atacatctgg agcagttgag catctaggag ataacagaac tggcgaaggt
      541 gatggagatg atgaacaaat agttgttgat ttatctaaga ttcctggaga aataagcaag
      601 atttctttta ctgttacaat tcatgatgca acagaaagaa gacaaaattt tggacaagta
      661 agtaattctt atataagaat agtagataaa gacacaaatg aagagttaat aaagtatgaa
      721 ttaggagaag attttagtat agaaacagct atagtagtag ctgaaatata taagcacaat
      781 ggagaatgga agtttaatgc actaggttct ggctttgaag gtggtcttgc tgcattatgt
      841 ggaaactttg gaataaactt ataatttcaa ctataacaat aaactaaagg gggatatttt
      901 ccatggctat agtattaaaa aaaggacaaa aaatcgattt aactaaggga aatccaggtc
      961 ttaaaaatat aaaacttgga ctaggatggg atacaaattc atttgatagt ggctatgatt
     1021 atgatttaga tgtaagtgtt ttcatggttg gagaatctca aagagttgaa aaagatgaag
     1081 attttatatt ctacaataac ttaaaacatc catctggagc agttgaacac ttaggtgata
     1141 atagaactgg tgaaggtgat ggagatgatg aggaaatatt agtgaattta gagacaatgc
     1201 caaagcatat acaaaagata gctgtaacag ttacaatata tgaggctgct gaaagaagac
     1261 aaaattttgg acaagtaagc aactcatata taagagtatt aaattctgaa aatgatgaag
     1321 aaatattaag atatgactta ggtgaagaat ttagtataga aactgcaatt acagtatgtg
     1381 agatatataa gtataatgga gaatggaagt ttagtgcagt tggagcagga tttgaaggtg
     1441 gcttagaagc actttgtaga aactatggct taaatgttta atttaagcaa tataatatat
     1501 taaatataaa taacctatac aaataatgta taggttatat tatataaaat tttgtggagg
     1561 tttagtatgt cgattaattt atcaaaaggt gataaaatag atttaaaaaa atctaaccca
     1621 ggactgagta acatacttgt aggtctagga tgggaccctg tgcaacaatc tggcggaggt
     1681 ttttttaaat cgttatttgg tggaggacaa gctgatatag attgtgatgc atctgtattt
     1741 atgcttaatc aagaaggtaa attaagtgga ataaaagatt taatttattt tggaaatcta
     1801 aaaagtgcct gtaaatcagt tttgcataca ggagacaatc taactggtga gggtacaggt
     1861 gatgatgaac aaatcctagt taatttggat aaagttccat caaatattca taaattattg
     1921 tttgtagtaa atatatacaa ttgtgtagat agaaaacaac actttggaat gatagaaaat
     1981 gcctatataa gagttgaaga ccaagggaat aagaaagaga tagcaaaata caatctatct
     2041 gataattatt ctgaaaaaac tactcttata gtaggagcta tatatagaaa agatggaagt
     2101 tggcaattta aagcaattgg tgaaggaaca aaagatgcgg gtcttaaaga agtaatgcaa
     2161 aatttagaca ggattgagtg tgcatatgga atttaataat gatatcaaat attataatgt
     2221 agataaagaa gaagttacaa aagacttatc tgttaatcct gaaaaaggtt tatcagaatc
     2281 agaagtcaag actagaagag aaaaatatgg tttgaatgaa tttactccaa aagaagaggg
     2341 tagtttttgg gatgatttaa aggaaagttt aagtgaacca atgatagtta tcttgattat
     2401 tgcagcagta gtaagtgcag taattgggga aactcatgat gcaataggga ttgtaggtgc
     2461 tatagcaata ggtatagcaa taggtatgat tacagaaggt cgttctaaga aagcagcaga
     2521 ggctctttct aagttgactg aaaacataga agtaaaagta ttgagagatg gggaagtaca
     2581 tcaaatttct aagagtgaat tggtaccagg tgatatagta tatattgaaa ctggtgacat
     2641 ggtacctgct gatggtagac ttatagaaag tataaacctt aaaattagag aagatatgtt
     2701 aacaggagaa tctgatgacg tatctaaaaa gtgtgatgta gtagtttcta tggaagagat
     2761 agaatctaaa ggagcagtag tagtacagga gccaatacct gcaaaacaga taaatatggt
     2821 gtttggtggt acacttgtag cttatggtag aggagctcta gttgtaactt caattggaga
     2881 taatagtgaa atgggtaaaa ttgctcaaaa cttatcagaa acagatgagc aaactccact
     2941 tcagataaaa cttggaaatt taggtggttt gatagccaaa gtttctagtg cagtagcagg
     3001 attactattt atatttatgg ttttccaaat gataagtaag aatgtattaa atgtagatat
     3061 gtctggaata ttaccgtttt tagaatcaat agacccagtt aaaacagcat ttacagtatg
     3121 tgttgcgttg atagttgcag cagtgccaga aggtcttcca actatgataa atatgacact
     3181 tgcaataaca atgcaaaaaa tggctaaaat aaatgcattg gttaccaaaa aagaagcttg
     3241 tgaaacgata ggttctgttt ctgtaatatg ttcagataaa actggtacat taactgaaaa
     3301 tagaatgact gtagaagttg catatgttga tggcagatat attgaaagtt cagaagagga
     3361 aataaatagt tattttgaag aaaactgtat gataaactct acagctgatg tagagcacaa
     3421 tgatggtgat attaagtatt taggaagtgc tactgaatgt gcattgcttt tatattacaa
     3481 aaacgtagat tatagacaag ttagaaaaaa ttctaatata gtagctcaaa atccttttac
     3541 ttcagattca aagagaatga cttctgttat aggtcaagat aatcatcatg tactattatc
     3601 aaaaggagca ccagaagtat tacttgaatt atgttcacat attcaaagag gaaaagacat
     3661 agttccatta actgaaaata taaaacatga gattttagaa gaaataaaga aattgcaaat
     3721 aaaatcaatg agaactttag ggtttgcata taaagaaatc tcacaagctg aagaagaagc
     3781 agcagtaact gctgaaagtg atgctatgaa tgtaagtgca atggaaaata atttagtatt
     3841 tagtggtttt gttggtataa gagacccact tagaaaagat gttatagagt ctgtaaatac
     3901 agcaaataaa gctggtgttt ctgtaaaaat gcttacagga gataatataa atacagcaag
     3961 agctataggt gaagagttag gcctattaaa aaataatatg agagtggttg aagcttcata
     4021 tatagatact ttagatgatg aagaattaaa acaagagata caaagtattt caatcgtggc
     4081 aagaagtaaa ccagatagta aaatgagaat agtttcagct ctgcaaaaga gcggagatgt
     4141 tgttgcagta acaggtgatg gtataaatga tgctccagca ctttcaaaag cagatgttgg
     4201 tatagcaatg ggtatatctg gtacagaggt atctaaaaac gcagcagaca taattttaac
     4261 tgatgatagt tttagtacca ttgtaaaagg tattaaatgg ggtagaggaa tttatgataa
     4321 cttccaaaga ttcgttcaat tccaattaac tgtaaatgta attgcatttt tagtggctat
     4381 aatatctcaa gttatgggac aagagatgcc atttactaca atacaattat tatgggtaaa
     4441 catcattatg gatggaccac cagcactagc attaggttta gagccagtta gggaccatgt
     4501 gttaaataga aaacctgttg atagaaaagc caatataatt tctaagtcaa tggtgtatac
     4561 aatagtatta aatgcatttt atataacagc aatactaatg ttacaatcta catttaattt
     4621 cttaggagtt gatgaagcgc ataagggaac agtaatgttt gcgttatttg cttttagtgc
     4681 actgtttaat gcatttaact gtagagagtt taatgcagat agtatcttcc ctaattttac
     4741 acataacaaa ttagcactac aaataatagt gttgacagga attgcacaag taatatttac
     4801 tcagctattc caagatttct ttaactctgt agcaatggat tttatgacat gggtaaaagt
     4861 tttatgtgta gcagcaagtg taattgtggt taatgaagtt gtaaaatgta taataagact
     4921 ggtaaaacca aaaaagactg cttaaaacaa attttttaga agaaaacaga ctgactcaaa
     4981 atatgaattt tgggtcagtt ctgaataaac atttaaattg ggaggatagt tatgggtgac
     5041 tcaaaggtat ttgaaaaaat attctccagc caatcagata gaggaaatta cactccttca
     5101 aagggatatc taagttattt tatttcgtat attggtttag aagatgaagt tttgtacaat
     5161 ctcgagatat ttaagacaaa acaaaatatt gatagcaaaa aagatattgc tttatttact
     5221 gatgttatag caaatcctag tgattttgat attataaatt attttaaaag tggacttcaa
     5281 aaatatagaa ctagcatgga agatgtagat ataaatattt tgggatttga agagattgat
     5341 tataaaataa agcaagcaat ggatagagtg ttaaaagaag aggaaaaaga atttactaat
     5401 gatagagtta aacaaaattt tatagtgaaa ataatggcat ggataaaaat ttatatcggt
     5461 gctttagata taaataaaaa tgaagctcca aaagtaatat tttatggaga tataaaaaaa
     5521 catgaagttt atttactctt aatattatac ttggcaggtt ttgatgtttt atatcttaat
     5581 ccaaattcaa agagtaatat aaatatttta aaatcagaaa gatacaatat agagtttgaa
     5641 gaagccaaca taatagaaga aaaaatttct tttgaagaaa gggttatttt aggggaaaaa
     5701 atagataaaa gttctgtaaa aaaagctttt actgttgggg cagaagcatc aaagcgtata
     5761 agtgaggaat tgttaaatga tgcaggattt ataaaacctt ggcaactcca agatagaaaa
     5821 ataaaaaatc tacttctaag ttcaactgtt gatgaaatat caatatactg gaatcaacca
     5881 cttaaattaa gaccaggatt taagtttaat gatgctattg tagaagctcc aaattttctt
     5941 tctaagatta atggaatata taacgataag aatgaatata ttaagttttt agatttatta
     6001 agagattcgg aaagttcaac atttattgaa tttaatggag atgtagatag attttcaaaa
     6061 gcttttacaa gagaagcttt tagtttatcc tttttattgg attcaaaagg tgcaatagat
     6121 aaaaactctg ttttaaataa taaagattat tccatatcaa cactagcatt gaatcaacaa
     6181 attatgattt tagagaaagt ggaagagttg ttagaaggaa gtatgttttt gaatggttta
     6241 agtggagaag acaagataaa aggtctattt acagtacttc acatggataa gaaatttgtt
     6301 catatgatga acaatttcga ttactcactt ataaacccaa aattgattat atacatgtac
     6361 aagagtattg tatttgataa agaaatagtt tttcttatgc ttttacttag taagattgga
     6421 tttgatatta tcatattatg tccaggagga gaaaacaata tagagaatgt tataaataac
     6481 caattgattg atgtacacag attggataaa atggtatatg accttaaatt aaattcgctt
     6541 gaaaatgata tacctttact taaaaaaatt tttggaaaga ggagacgatt ttagatgggg
     6601 ataaatataa atagtttttc aaaagataag aactctgtag aggttactga gaatttgcct
     6661 acagcagttc aagaagaaaa cttcgatata atggaatata caaataataa aaaaaatgaa
     6721 ctgagaaagt ctaaggaagt tgaggcattg acatctctta tagaagtaga aaatccagat
     6781 acgattttac agtttggtag gaaagcatct gaaggagtag caagagtttc tgatagcctt
     6841 ttaaatacaa taaaattaaa tagaaatgaa gaaaattcta aaatgctggt tcatttaact
     6901 aaaataatgg acaagtttga tttagacgat tttcaagaaa ctaaggaacc aaatttcgtt
     6961 caaaaacttt tcaaaaaagc taataatgcc attgaaatga tgtttcaaaa gtatgaaaca
     7021 ttgggaggag aagttgagaa aattcaaatt gagcttgaac aatacgaaag agatatagca
     7081 ctttctaata agcaaatagg tgctatgtta aatgaaaact ttgagtttta caatgaactt
     7141 caaaaatata ttgttgctgg agaaatggca atagaagaaa tggataatga aatactacct
     7201 ttctttaaac aaaaatcaga gactagtgga gaccaaatgg atgttgtcaa ttatcaagaa
     7261 ctattaaaag tttatgatat gttaaatcaa agagtctatg atttaaggat agcagaaaat
     7321 atagctatcc aaacaatacc tatgcttaga ggtatgcaac ataataatta tggtttaatt
     7381 agaaaaataa atagtgcatt tgtcgtaact ttaccagtat ttaaacaatg tttatctcaa
     7441 gctatattac ttaaaaagca agaattacaa gcaaaatctt taaaagcact tgatgacaaa
     7501 actaatgaat tattgcttag aaatgcacaa aatgtaagta ctcaaagtgc acaaatagct
     7561 agaatggctg gaacaagttc tgttcaaata gaaacattgg aaaaaatgta caatactatc
     7621 aaatctggta tagatgaaac tatgagaata gaagaaaaca atagagcttt aattaaggat
     7681 aatacaaaga gattggaaga attaaatacg actataattt ataataagta gtattatatt
     7741 aagggctaat ttgtataaag ttggcccttt ctatgtatat gaggaggcta gacattgaaa
     7801 tatttcgatt atatctgcaa agatgatttg gagagaattt ttttgaagga gccagaagat
     7861 tttagtgcta agactgaaaa agatgtgttg aaatatgcac tgggcgcttt tttatacgtt
     7921 ccagctactc aatataatat gatttataag agtattatag gggatgttaa gggagttaga
     7981 ccattagcta tttgtttaga agatgctgtt ggagtaaatg gagagttaga ggctatagag
     8041 aatcttagat taattttaaa aaatattagc aatgaaagta taactaataa agatggaata
     8101 ccactaattt ttgtaagaat aaaagatgtg gagcaactgt tgagaattaa agagattata
     8161 attaaaaata gtcattttat aacaggaata ttaattccta aagcaaattc ggagttaatt
     8221 gaaaattgta tagaagcttt agattctatg aatcttcaag atatgtatgt tattccgata
     8281 gtagagacaa aagaatttat atataatgaa aaaaaagaac ttagttttac taatttgtac
     8341 aatgctattt taagacataa gtcaagaata ttaagcatta gagtaggcat aactgatata
     8401 ttagggatgt atggtataag gagagataaa aacttttcta tatataataa tttgatttgt
     8461 tcttcattta tattagatat aatcacatat cttcaaagac cagagttaga tatacctata
     8521 agtggtggag tatcagaatt ttttgatatg acgaataaag atataagaaa taagtatata
     8581 gaagaaatat tattggataa atatcatgga ttaattggaa agaccgtaat tcatccaatg
     8641 caaattcaaa ttgtacaagc gctatctaca gtatcatatg aagattttac tgatgctttg
     8701 gatatactgg atagtacaga cagtaagtat ggtgtgagca agggtgtatt aggagagaga
     8761 atgaatgaaa caaatccaca ttttttatgg gctaaaaaaa cattgattct atctaaaata
     8821 tatggtgtat taaataaagg agtagactat gaagaattgc ttaaatttta attgtgagat
     8881 tgacattatt gataatccat tgaatttaga actaaatgat tttttagacg taggaataag
     8941 aaataatagc aaaagacgtt ttttgtttat ctcaaagaaa ctcggtaaac atttagcatg
     9001 taagccaagt gagatggata atttgggaaa attaatggct acaatttata atgaaaaaaa
     9061 taaggaagaa tcaaatggta ctgttatagc ttttgctgaa actggtacag ctgttgggca
     9121 tagttttttt gattacttat gtggagatta tgaatttatt catactacta gagagcagtt
     9181 tgacgagtta gaaaatctgg aatttttaga ggaacattcc catgcaacag accagaattt
     9241 atactttggt atgcttaaca actttaaatt tggagatgag ataatattgg ttgatgatga
     9301 gattaccaca gccaatacat gcatgaatat aattaggaaa attcaaagca tttatcctaa
     9361 aaagaaatac actatatgct caatactcaa ttgggtaagt gatgaaaatt taagtaaagt
     9421 tactcgttta gaaagagagc taaattgtaa gatagatttt gtatacttat ttaaaggaaa
     9481 ctttaaattt tatatagatg aagatattat ttcaaattac aacgataatt atagtattac
     9541 taaattagag actatttcaa gtgataaagt aaatttagta gtaaatcata tttatgttga
     9601 tttagaagat tatattggaa ataagaaata tgtaaaatat acaggaagat ttggaattaa
     9661 tagaatagaa caatttagat taaaagaaat cgttaagaga gaaagtaaaa aaataactat
     9721 ggccagtaaa gaatctttaa aaaatgaaaa agaaaagatt ctatttttag gtactgaaga
     9781 atttatgtat atacctatgc tgtttgcaaa agaaaaagaa aataaatatg atatatacta
     9841 tcattcaaca acaagaagtc caattattaa tattgataag aataattatc ctattaaaag
     9901 taaatttatg ctaaagagtt tttataatat agatattcaa aattatattt acaatataga
     9961 taaacataat ttttctaagt gttatttatt tgtagagctg aataaaagtg aaaattctta
    10021 tctagaattt attgatataa ttaaaaaaac aagtataaaa gaattaacta tagtatgttg
    10081 tagctaaagt gttttaacag gataaaatat atcacaattt taagatagga gttgagtcat
    10141 atgagagatt ttagcacgta ttctaaagat gatgttattt ttttattaaa agatatatca
    10201 aatttaattg aagaagaagg aaataaagaa agagaattaa aaatacaaaa tggtagacat
    10261 tattcagaaa tgataccaat agagtatgaa gttagtgaag attatctgaa tttatatcat
    10321 gagaaactaa gagaaaataa agataaactt gcattttcta taggagttat gtgtgaaaaa
    10381 ataattaaaa aaaatggaaa tgatgtagta ctagtttcac ttgctagagc aggaacacct
    10441 attggtattt tggcaaaaag atatataaga agcaagtata atataagttt acctcactat
    10501 acaatctcta taataagaga taagggaata gatatgaatg caattgaata tatagtaaag
    10561 aatcatccga gctcaaaaat tcaattttta gatggatgga ctggtaaggg aactatatca
    10621 aaagaacttg aaaaagcatg tgatgaactt aatgttaaat ttaaaaaata ttttgactca
    10681 acacttgctg tattagctga ccctgctgga tattcaggac tttatggaat tagagaagat
    10741 ttcttaatac caagtgcatg tttaaattca accgtttctg gtttagtaag tagaactgta
    10801 ctaagagagg atttaattgg taaaaatgac tttcatggag ctaagtttta tagacattta
    10861 aaagataaag atgaatctat gaactatatc gaaactatag aagaatgttt taaaaatcaa
    10921 tttaagaata tatctgatga agttgaaaac tgggaaagtg acattataac aagagatgga
    10981 tactttgatg ttttaaatat aaaagaaaag tataacataa cagatattaa ctttatcaaa
    11041 ccaggtgtag gggaaacgac aagggtttta cttagaagag ttccgtataa gattcttgtt
    11101 aaagacttaa atgataaatc tttagaccat atattcatat tagctaagga gaaaaatgtt
    11161 gaagttgaac aaatggattt aaaagcctat aaatgttgtg gaataataaa aaatatgaaa
    11221 gatatttagg aaatatatta gacaggagaa tattgaatga ttgttttttc agatttagac
    11281 aggagtatca tttactccaa taaattttta aatgctgata gtaaatatac taatatagaa
    11341 atttataggg aaaaagaaat atcttatata tctttagata ctataaattt aataaagcaa
    11401 atacaatatt atggtatgtt tatacctaca actactagaa cagtggagca atttaagcgt
    11461 atagaattta ataaatatgg gatttatttt ccttggagta taacttcaaa tggaggagtt
    11521 atacttaaag ataatgaaat attgaaatca tggagtgaaa aaatagataa actaaaatct
    11581 gattatgagc ccattgaaag tatgattcat aaatttaaag actatttgaa tgtagatgga
    11641 ataactaatt ttaaagttgc agaagataca tttttttata tagttgttga tttatcaaga
    11701 tttaatttgg attcaatcaa agaatataca aatatattgg aatcaaaaaa ttggaagttt
    11761 tatgtgagtg gcagaaaaat atattttata ccaaaagaaa tttcaaaaga aaatgctata
    11821 aaatacttga ctaaagaatt aggaatagaa tatttttatg cagttggaga ttctattatg
    11881 gactatggta tgcttaatat ctcaaataaa tcctatgtat tgaagcatgg tgatataaat
    11941 aaaaatgaaa tagaaaattc atttatatca agttctttca atggaatgag tggaactgaa
    12001 gagatactat ctaacatttt agacgagaac tgcctactta acatataatg actgatatat
    12061 aaataataaa ctagaaaaat ctgaaataaa ttatagtaaa gtttatcata ctaatttatt
    12121 tcagattttt atcaatttat atttaagtaa gaactcaata tgttaaaatg aaggtggagt
    12181 cattgcccag actgagatac atacctcatc atcataattt tcatacacat gtggtatagt
    12241 actgttaaaa taaaaactat ctccttcctc caatagatat tccttatttc caagtgttat
    12301 cttcatttta ccttttatta tatagccaca ttcttctcct tcgtgtgtca caaattcact
    12361 gtttttttgt gtatgaccat ctagcgtaac aagtataaat tctatttttt tcccagataa
    12421 attaggaaca agaagttcat aaaattttgt agaatcatta gttactattt tttttcgatt
    12481 atttttcttt actactatat cagagtcttc tcttccaatc tcttcaaaaa aataacctat
    12541 atttatatca agagcttttg atattttcca cattgctaca atagatggaa caaccatatt
    12601 acgctcaatt tgacttatta agcctgtact taaatctgaa ttttttgcta aagtagagat
    12661 acttatattt ttagattttc taagagatct aattttgctt ccaacatcta tattcattag
    12721 atacaaaccc cctcagacat gattataaca tataatttat aaagattaat aaaataaaaa
    12781 aaaagtttta caatacaagt attttttatg ctatattgaa ttttctgaaa agatgcatta
    12841 taatagtatt aatattcaat atattgaata ttgataacga tattttaatt taatgaaatg
    12901 gaggtaatta agatgaagag ttttgtaagt cctgataata tttgggtatt atgggcagtt
    12961 gtaactggat gggcagcatt tagtatttat ttggaacaaa agtacaattg ggcatcaaaa
    13021 gtgtcgggag caataatagc tcttgtagga gcaatgttat tatcaaatct aaatattata
    13081 ccagtagagt ctgttgtata tgaccaagta tggggatatg ttgttccttt agccatagct
    13141 ttattgttat atcaatgtaa cattaaaaag atatggaaag aaagtggaag attgctcatt
    13201 atatttcttg taggttctgt aggaactgta cttggagcta tgattggatt tttggcatta
    13261 aaaaatgtag taccagattt aaatattgtt gcagctatga tgacaggttc ttatataggt
    13321 ggaaatgtaa actttgctgc tatgtcaggc gcgtttgatg cgccaggaga attagtatca
    13381 gcaacagttg tagcagataa tcttttaatg gcattatatt tctttgtctt aatagctata
    13441 ccatcaattg gattttttag aaaacatttt aaacatccac atgtagatga aatggaaagt
    13501 ataggtataa ctgaaggaga aacagtagca gcaaattatt ggggaagaaa agaaatatct
    13561 ttaaaagata tagcaatagc cattggtagt gcttttataa ttgtcgcagt atctgtagaa
    13621 ctatcaactt ggtttaaagc tataatacca ttatcaaatc cttttttagc aatgttaaat
    13681 actttatttg gaaatcaata tctaataata actactttga ctatgttgtt tgccacattt
    13741 atgcctaatt tctttggtga tataaaagga gcacaggaat tgggtacatt tttaatatat
    13801 atattctttg ttgtagtagg tgttcctgct tcaataacat taataattca aaaatcacca
    13861 ttactacttt tatactgcgg aataatggta ctaataaaca tgctagttac atttatagtt
    13921 gcaaaaatat ttaagtttag tttagaagaa gctattcttg catctaatgc aaatattggt
    13981 ggtcctacaa cagcagcagc tatggcaata tcaaaaggat ggtctaagct agtagggcca
    14041 atacttatag ttggaacatt tggatacata gtcggtaatt attttggtct tttagtagga
    14101 aatattttga tataatttta cgtgaaaaaa agttgttttt tagaaagatt tttatttatt
    14161 agaaagattt ttatttaatt ggaggttgtt atgattttcg atggacattc agatacttgg
    14221 gcagatgtaa caataagaag gcttaacggg gaaaaagatg tatttagaag aatccatctt
    14281 gataatttta aaagagcaaa tatagaagga gctatttttg taatatggat agacccacca
    14341 tatgataaag agcctgaaaa aagagttaaa caaattataa gctctataaa agatgaaatt
    14401 gaagatatga aagatgttgt aaacatagta aaaaaatatt cagatataga taaaggtata
    14461 agtgaaaaca aaataaatat tttaattggt cttgaaggat taagccatat tggaagaaat
    14521 atagatttac tagattattt ttataaagaa gttggtgcaa gacatgcatc attaacttgg
    14581 aatgaacaga atgaacttgc tacaggatgt ttaggggatg aaaatcgtgg tttaactgaa
    14641 ataggtaaaa aagcagtcaa taaaatggaa aaattgggca tgatagtaga tgtgtcacat
    14701 ttaaatgaaa aatcattttg ggatttaatg tctgttgcaa gtaagccagt tatagcttct
    14761 cattctaatg ctaggagttt atgtgatgct aaaagaaatc ttacagataa tcaattaaaa
    14821 gaaatttcaa atacaggtgg attagttgga ataaatagtt ttagaggatt tactaatagt
    14881 aatcctaaga agcaagattt aatacatttg gttgaccata tagactatat ggtaaatctt
    14941 ataggtatag aacatgtatc ttttggtttt gattttagtg attacttagg gaaagagact
    15001 ataggctctt ttactggaaa tgctagtgtg ccaagtgtag atggtcttga aagcacacat
    15061 acagcacaga acatagtagt agagcttgaa aatagaggtt ataaaaagaa agatatagag
    15121 ttaatctcta gtggtaattt ttataggctc tttaaagata tactaaaata atataataaa
    15181 gaacttaaat ttataaaata gtatatttat acaaataata tctacatatt taaattggcg
    15241 ttcataattt attaaatttt aatgttacta gaagtttcaa ttctagtaac attttttatg
    15301 ttaaaaacag atat
//
LOCUS       DAELJO010000082        14735 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELJO010000082 DAELJO010000000
VERSION     DAELJO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.082"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            140..1426
                     /locus_tag="KPB88_002138"
     CDS             140..1426
                     /locus_tag="KPB88_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0054414.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1512..2819
                     /gene="grdB"
                     /locus_tag="KPB88_002139"
     CDS             1512..2819
                     /gene="grdB"
                     /locus_tag="KPB88_002139"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2559..2561,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0054415.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            3097..3828
                     /locus_tag="KPB88_002140"
     CDS             3097..3828
                     /locus_tag="KPB88_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054416.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            3830..5188
                     /locus_tag="KPB88_002141"
     CDS             3830..5188
                     /locus_tag="KPB88_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0054417.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYHFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTRGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            5227..5712
                     /locus_tag="KPB88_002142"
     CDS             5227..5712
                     /locus_tag="KPB88_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054418.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(6367..6591)
                     /locus_tag="KPB88_002143"
     CDS             complement(6367..6591)
                     /locus_tag="KPB88_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0054419.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(6688..7941)
                     /locus_tag="KPB88_002144"
     CDS             complement(6688..7941)
                     /locus_tag="KPB88_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG0054420.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            8201..9190
                     /locus_tag="KPB88_002145"
     CDS             8201..9190
                     /locus_tag="KPB88_002145"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG0054421.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKAGRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            9262..10002
                     /locus_tag="KPB88_002146"
     CDS             9262..10002
                     /locus_tag="KPB88_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG0054422.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            10218..11369
                     /locus_tag="KPB88_002147"
     CDS             10218..11369
                     /locus_tag="KPB88_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0054423.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYELGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            11394..12149
                     /locus_tag="KPB88_002148"
     CDS             11394..12149
                     /locus_tag="KPB88_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG0054424.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNNNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            12341..14728
                     /locus_tag="KPB88_002149"
     CDS             12341..14728
                     /locus_tag="KPB88_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG0054425.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         5852 a         1607 c         2533 g         4743 t
ORIGIN      
        1 taaattaaaa atgaaaattt ctatgacatt aatacataga atatatattt taaataattg
       61 gcatagaaaa tgctttatat attaataaac gtaataaata cttttaattg cgattaaata
      121 tattaagggg gattttcata tgaagctaga gctagggaat atatttataa aaaatgttga
      181 atttggagaa aagacagaag taaaagatgg agtactatat attagtagcg aagaaataga
      241 aaagatagta ttagaagatg aaaggattgt aagtgtaaat gttgaacttg caagacctgg
      301 cgaatcaata agaatagctc cagttaaaga tgtaatagag cctagagtta aaattggaga
      361 tgaaagcaaa atatttcctg gaattataaa taaagtgaag actgtaggaa gtggaagaac
      421 ccatgtactt ttaggagctt gtgttgtaac ttgtggaaat atagttggat ttcaagaagg
      481 cgtaattgat atgagtggtc ctactgctaa atatacacct ttttcaaaaa caaataatat
      541 atgtattgtt ataaaagcaa aggatggtat agatactcat gattatgaag aagcagctag
      601 aattgcagga ttaaaaatag gtgcatatgt aggtgaggca ggaagagaag ttgagccaga
      661 tgaagtcatt gtatatgaga caaagccatt attaaaacaa gtaaaagaat atccagacct
      721 gccaacagta gcatatgttc acatgttaca atcacaagga cttttacatg acacttatta
      781 ctatggggtg gatgctaaac agatagtgcc tacatttatg tatccaactg aaataatgga
      841 tggagctata atatctggaa actgtgtagc accatgtgat aaggttacaa cattccatca
      901 tttaaataat ccagttatac atgacttata taaaagacat ggaaaagatt taaactttat
      961 aggagttatt ttgactaatg agaacgtatt cttagtcgat aaagagagaa gctctgatat
     1021 ggtagctaaa ttaatagaat ttttaggagt tgatggagtt ctagtgacag aagaaggtta
     1081 tgggaatcca gatactgacc taatgatgaa ttgtagaaaa tgtagtgaag ttggagcaaa
     1141 tgtagttttg ataacggatg aatttcctgg aaaagatggc aagagtcaat ctatagcaga
     1201 cgctactaag gaagcagatg cagttgtttc ttgtggtcaa ggaaatctag tggttcattt
     1261 cccagcaatg gaaaaaatta taggaacttt agattatgtt gaaatgatga taggtgggta
     1321 taaaggatgc ttaaatgaag acggaagcat ggatgcagag ctacaaatta taatagcatc
     1381 aactatagca aatggctata atcatttgac agcaagatat tattaaataa gtatttgaat
     1441 ataaagttct gttgatgtaa tcattggtct aattgatgta ataactataa agtgatttgg
     1501 aggattaaga gatgagtaaa atcagagtgg ttcattatat aaacaatttc tttgctggta
     1561 taggtggaga ggaaaaagca gatattccgc cagaaaaaag ggctggtgta gtaggacctg
     1621 gaatagcatt tcagagtcaa tttaatgatg atgcagagat aatctcaact gtaatttgcg
     1681 gagatacgta ttttggtgaa aatattgaga gtgcaactca agaactttta aaaatgataa
     1741 aagaagaaag ccctgattta tttattgcag gcccagcatt taatgcaggt agatatggag
     1801 tagcttgtgg gactatttgt aaggcagttg aagaagagtt aaatattcct gtaattacag
     1861 gcatgtataa agaaaatcca ggtgtagata tgtttaaatt agatttgcat atagtgtcta
     1921 caggaaattc agcagcaagt cttagaaagg tagttccaat aatgacaaaa ctaggtctta
     1981 aattggttag aggtgaggaa ataggacctc ctgaagagga aggatatata atgagaggaa
     2041 taagaaaaaa tttcttccat gaacttagag ggtctgaaag agcaattgat atgttggtta
     2101 aaaagatgaa aggggaaagc tttgaaactg agtatccaat gcctgaattt gatagagtta
     2161 cacctatgaa acctgttaaa gatttatcta agataaaact tgccctagta acttctggag
     2221 ggatagttcc aattgataat ccagacaaaa ttgaatcttc aagtgctact aaatatggaa
     2281 tttacgattt aacagatatg gattctatgt caaataaaga atttacaact atacatgggg
     2341 ggtatgacag agcatatgtc cttgaaaatc caaatttagt tgtaccacta gatgtagtta
     2401 gagagcttga aaaagaaggt gtaattggag aattagtaga ttattttata actactacag
     2461 gaacaggaac gagtgttggc aattctaaga gatttggaga agagttttct aaaaaattgt
     2521 tagaagatga tgtagatgct gtaattttaa cttctacatg aggtacttgt actcgttgtg
     2581 gagcaacgat ggtaaaagag attgagagag ctggtatagc agttgttcat atatgtacag
     2641 tagtaccaat atcacttaca ataggagcta atagaattgt tccagcagta gggataccat
     2701 atccacttgg aaatcctatg ttaggagaag aggaaagtaa aaaaattaga aagaatatag
     2761 ttttaaaagc tttaaatgca ctaacagaag atatagaaga acaaacagta tttgaataat
     2821 aaaaatacaa cacaacactg aaataaaaaa agatatcaaa aaataatttt ttaaaattat
     2881 tttacgatat ctttttttta ttaggtaaat tcgaaatttg cattttttta aattaaattt
     2941 ataaaagtga taaattagtt tattataata ttgatacaca aattcaaatt agatatacta
     3001 tgaaaagcga gattattaca ttttggaaca aatattcaac atattagatc atattaattg
     3061 aacagaaaaa attaacttta tataataatt agaataatga taagaaagaa ggtgtatata
     3121 ttgatttcaa ttgccatttg tgatgatgaa tcctatatgc taaatcattt aaaggaaaat
     3181 atagaattat acatgaagca gaagaattta ttatataata tagagctttt taataaagta
     3241 gaagatttac tatgcaatca tgaaaagttt aatattgtat ttttagatat acagatggaa
     3301 gagattaacg gaatgcaggc agctagaaaa ttaaggtcat atggtaatga gtgctttata
     3361 attttcgtta cagttttaaa tgaatttgta tttgatgctt ttgaagtaga tgctataaat
     3421 tatctgataa aacctctatc taatgagaaa ctatataaaa ctttggataa aatagttaca
     3481 aaacttgatg ataatgaaag taattatatc acaatacaaa aaggttattc atattggaaa
     3541 ttaaaacttg ctgatatttt atattgtgaa gttataaaaa gaaagattta tgttcattta
     3601 aagaaagaga ctgtagatta ttatgagact attgaaaatt tagagagaag tttaccaaca
     3661 aattttttta ggtgtcatcg tagttatata atcaatttac aatatgtata tggatatgaa
     3721 aatggtaatg cagttatgga aagtggagat agaatacctg tttctagact acgtcaacaa
     3781 atattttcaa aagttatgct tgagttcatg aaaaatagga ggatataaga tgattaattt
     3841 attttcttcc gatttatttt taaagtacag ttttttcttc tcttttattg tatatagaat
     3901 tttaatggta atagtgcaat accattttct aatcaatttt acaaatttta attcaaaatt
     3961 ttggtgtttt attttatatt cgataatttc aattttttta atcattataa atacatattt
     4021 taatttatat aatttatttt cttcacagat tctatttttt cttatgactt ttttatttgt
     4081 aaagtacata ataaaacaat ctactgtttt atctatagta tcaactttgt tagtatattt
     4141 aattgaacaa ttagtatatg gatttacagc tccattaaat tacttgatat ttactagtga
     4201 tataaataca gatatttata aatttgatat aaatatggga ctatcttctt taattactat
     4261 tataatagct ggatttatat attggtatag ttctaaaaag tttaatatta aggtcatgaa
     4321 ctttgataaa tatatagcaa ttttgatgat tccattgttg cttataatat tatttatgca
     4381 atcatttgaa tatagttcaa atataaatat agatacaagt cttgggattg taaagctgct
     4441 attaaatact tctataactg aagaaattca agcttttctt ttttctataa ttggaactat
     4501 atgtgtattt ttttctctat ttacctttaa gaaactaatt caagctctcg aagatgacaa
     4561 ggagagggca attatgaatc aacagattca tgctcaaaaa aactatattg aagaagcgaa
     4621 atcacgatta tcacaaacta tatcctttag acatgatttt aataatcatt tagctattgt
     4681 caatggattg ttgaaaaaag accaaatttt aaaagcacaa gattatttaa ataagcttga
     4741 aaaagtaaca agtgacctat cattttcttg taatacacag aatattgtta ttgatgctct
     4801 tttcaacaat aaattaagta tagcaaaaca attaggcata gaggtagatt gtgaagtaat
     4861 tataccacaa agcactaaca tgaacgattt tgacctatgt atagtgtttg ctaatgctat
     4921 tgacaatgca attaaagctt gtagagttgt agataaacaa aataggtatt taaagctttc
     4981 cactaaatta gagggtggat tttttatgat agaaataaaa aatagctata atccaataaa
     5041 taattataca cgtggttcag gtataggtct tttaaatata aaagaaattg ctaaaaaata
     5101 tcaaggggct ataagtatag ataagacatc agattatttt caaataaatg tattgctagt
     5161 tatttcatta cattgcgcta gtatttgaga acaatttcat tgaaaatagt atttgtaagc
     5221 tatattatgt atttaaatag agaaagtttg atatatagaa atgaggaggt tactaaaatg
     5281 aaagttggaa atataaatgg tcaagcacca attacaaatg gtttagatag ttgtcaaagt
     5341 gaggtacaag gcttagaaca aaagaaaaaa gcattacagg atgaaataga gaaagtaaga
     5401 agcgataaaa gtattgatgg tgaaacggcc aaattaaaaa tagaagcttt aaaaaaagaa
     5461 atggaagaga ttgaaaataa aattcaagag ttaaaaggaa ataaaggaaa aaaaacaaat
     5521 aaagaagaaa ataacgtttc agaagtaaat aataatctat tagagagaaa aaaagaacct
     5581 tctaataatg aaaatgtaaa acaattatat gatgtttttg aaagaagcaa tgtagacgaa
     5641 caatgggata atacttatcg tgttctccaa gatgataaaa acttaaaagt aaaatttaat
     5701 agacctatat agtttattta taaaaatagt taatatttta attatcacca ctttttagaa
     5761 gaatacacac tttattttaa agacccacat atttatttac agttagcaca tttatatgtg
     5821 gttttataac tattatttac aagtctatat tgataacttt tacatagtat ataatgttta
     5881 aatacttatt caaatttatc tatacataaa caaaaaaaga gattttaaaa tctcttttta
     5941 gattgttgac ttatggcttt ttgatttaat aaaatcaaga agccgtttga attttatctg
     6001 ttggctgata cccttctgat attgaaacac catttttgtt tccaaaatcg tttaaaatta
     6061 agaacttatt atgaatgact atttcattat gaacaataag caaataacat gaatcttcaa
     6121 tcaatgtttt aacagtagca ttaaatgctt gcttatatag ttctatcgtt tgtttgaact
     6181 gcccattaaa gtaaatatga gtcaataaca taactatatc tacacaaatt aatataaatt
     6241 ttttatactg tacattatct aactgttgat tatctgtatt acaacatgat tgaatttaaa
     6301 aagccatcaa cacatagaat gttgatggcc tgaaaataga ttttaaaatc tattttttat
     6361 tatatattaa ttatttttta aatttatatt ttttgcatca gattttctaa tagctaagtc
     6421 aaaccctcta aatctaatca ctatagggtc attaagagga gctatctttt tcacttcaac
     6481 ttcagtgcca tctattagtc ctaaggctag aagtctcttc ataagttttt catttccagc
     6541 tatattgtct ataattccct tttgtccaag ttttaagttg taaacagtca ttattaacac
     6601 ctcctaatga aaatcattat caaactttat atttataata tataacaaaa agagcaaatt
     6661 gtcaatatat aacaaaaaat aaacatacta agttagtatg tttataaaaa atagtataca
     6721 tgggatacta atagcatcta gcaaaaaacc gcttactaat ggtacaacca aaaaggcttt
     6781 tggagaatca ccatattttt gtgtcaaaga actcatattt gccaaagcgt tcggagtagc
     6841 tcctattcca tgtccaatca aaccacttat tatgatggca gaatcaaaat cttttccaag
     6901 tactctaaag caaataaata ttgaaaatag gattataaag attacttgaa aagttactat
     6961 taagagaata ggaggtaata atgtagataa ttcaaataaa tctatattca taagtgataa
     7021 agttaaaaaa attcccaatg aaagttcttt taaaaaatct ataagtttaa aatttaattc
     7081 tagtatattt atcttatcat ttaaatttct aaaaataaca gccgaaaaca tacaagttac
     7141 aacagagggt aatagtatat taaacgttat atagaagcat ctacttataa tttctcctgt
     7201 atttatacaa ataaatatga gaagaacctg ttccaaaaat acagatggtg ttattgtttc
     7261 ggagaagtta ttatttttta tcctattctt atttctaaaa aatttacttc caaatctctt
     7321 tagatttcta ttatatctag gaatcgacaa gtctatattt gatgatggtt ttaatttata
     7381 tttatttatt agaaatttag ctacaggaca tcccaatata ccacccataa taagtcctat
     7441 tgttgcagca gataatccaa ccccaactgc tccttctata ccgagatttt ctattgtaag
     7501 tccaaatgca gcagaataac catgaccacc ttccatagat acatttccac acataagccc
     7561 taaaagaggt tcaagtttta taatctttga tattgtaata gctaatatat tttgacaaaa
     7621 tgccaatact ccacacaaaa tccaatatct aaataataca ctaccccctt tctttataag
     7681 atttatgctt atttcgagtc ctatagttgt aaaaaagaaa gttataaaat ttggcattaa
     7741 atttccactt atagatatat ctgctatatt aaaatattta aaaaataaat ttagaaaaca
     7801 aaataataga cctcctatta ctggtgcagg tatacagaaa ttatttaaaa agttaacttt
     7861 attcttcaat aaattaccaa ttaaaaagaa gaatatagat agtgtcaagg tttgcactat
     7921 atcaaaactt aaggtaatca aaataagtcc tcctttgtaa ttcaaagttt taattatata
     7981 acttaaattc ttatgtattg aaattttatt atcaaatgta tattaataaa tgtatattaa
     8041 aaagaatcag tagatataat tagatagtca taaattaaat taaaatattt taatgagatg
     8101 tgactttttt agaatgaaaa accgtagtat attgatataa tagaaataag acttaaaaat
     8161 ctgttgatag tattatttat aagtatagta ggaggataaa atgtttgaaa atttacctaa
     8221 gatagattta cattgtcact tagatggaag tgttagagta gaaaccatgc ttgatatagc
     8281 tattaaagag aaaatagatt taccttcaaa taatatggat gaaataaaaa aattagcaaa
     8341 ggtttctttt aattgtactt cactagatga atatttagaa aaatttgatt tacctttaaa
     8401 agtaatgcaa tctaaggaaa atttaaaaag aattactttt gaacttttgg aagatgcaag
     8461 tagagaaaat gtaaaatata ttgaaattag atttgcacca ttacttcaca cccaaaaagg
     8521 tatgagtgtt aaaaatataa ttgagggtat aattgaagga attagagaag ctgaaagtat
     8581 ttatgatata aaaggcaatt taatattagg ttgtatgaga acaatgacct caaaagaggc
     8641 attattagta attgaagaag gaaaatcatt tgtaaataaa ggtgttgtag cagtagactt
     8701 gtgtggtcca gagaaagagg gattttgtaa ggaatataaa gatgttttta aattagcaag
     8761 agaatatgga tataaagtta caatacatgc aggagaagct gcaagtggag aaaatgtatt
     8821 ggatgctata aatattttaa aggcgggtag gattggtcat ggggtaaaaa ttaaagacca
     8881 taaaaaggcg tataatttag ttaaagataa aaaaatattg cttgaattat gtccaactag
     8941 taatgtacaa actaagacag tagattctta tgaagcacat ccattttata ctttttataa
     9001 agataattta catgtaagta taaatactga taatagaact gtatcagata taaatttaag
     9061 ttcagaacta aatgttatat ttgatacttt taaactagga ttagaagact ataaaataat
     9121 ttatagaaat gcagtagaag ctagttttgc agataaagaa actaaagagt atttgaatag
     9181 tttaatttaa tagataaaaa tatttaaact ttaaattaag ctattaaatt gacaaaaata
     9241 cattgtatta ggagaaaaaa tgtgaaagat tatgaagatg ttgtttatat taaactagtc
     9301 aaaaaagaac ttgaagaatg gaagaaaaat atattaaaga aaccttctat atttgacaaa
     9361 gcatcaaagg gtatacagaa taaatttaat ggagtattgc cagataaata tcatgaaata
     9421 attacttctt ctataaaagt aatgacaaaa accgtactgt ttggatataa atatgctaca
     9481 aagccaccac ttagaaatat gtcattagaa gaaagagaaa aattagtttt agaaaaagct
     9541 aaaacttaca agactacagc aatgattgaa ggggcaggaa caggggcagg aggaatcctc
     9601 ttaggtttat ctgattttcc tttgctttta ggtattaaag tgaaattttt atatgatgta
     9661 gcttcaatat atggatatga tgttaaagat tatagagaaa ggatttatat attaaatata
     9721 ttacagttgg ctttttctag tcaacagcat ataaacaaaa cttttaaatt gatgaataac
     9781 tgggatgaat atgtaaatag tctaccagaa gacatcaatg attttgattg gagaacattt
     9841 caacaagagt atagggatta tttagattta gctaagtttt tacaactaat gcctggaata
     9901 ggagcatttg taggatttta tgtgaatgga aaattaattg ataaattatg tgaaactgct
     9961 gtgtctgctt atagattgag gattcgagag cttaagttat aagaaaaatt ataacataaa
    10021 aaattagtta tacaatatat ctatatgtat ctaattttgc attgataaaa acaattatgg
    10081 ataaaaggta aagcatgata gaatctaatg tagaggtttg tattttaatg gaaaaattta
    10141 gtttttattg cttaagatac aaattgaatt acgaagagat tgaattatga agagaagtaa
    10201 ttgggaggaa tattttcatg acaaatttgc caaaacaatt tgatagcttc aatgaagcta
    10261 gacaaaaagg atttataaat gcaaaggaac ttaaggaaag tgggaaaaag atggttggag
    10321 ttttctgtac atttactcca gttgaaattc caatggcagc tggagcaact gtagtaggtg
    10381 tatgcggtgt tagtgaagag ccaatacctg atgcagaaag ggtgcttcca agaaatcttt
    10441 gtcctttaat taaatcaagc tatggacatg caataacaga tacctgtcct tatttctatt
    10501 tttctgattt gttgatagga gaaaccactt gtgatggtaa gaaaaaaatg tatgaagaac
    10561 ttgcaaaagt taagccaaca tatgtaatgc atttgccaaa tacttcaaaa ggagaatttg
    10621 cctataaact ttggaaagat gaaatgataa gattaaaaga agaggttgag aaatctttag
    10681 gtgtaactat aactgaagaa gatataagaa ctgctataaa agataaaaat gaagaaagag
    10741 aacttttaaa agagttttat gaacttggta aattacaacc atctgcactt acaggccttg
    10801 agttacataa tgttttatat caagcaggtt ttaaatttga ccgtgcagaa cttaaacatt
    10861 ctttaagaaa agttatagat gatatgaaag aaagatatga aaaaggtgaa tgtcctgttc
    10921 aaaaagataa gccaagaata ttgataacag gttctcctat tggtggaata agcgaaaaaa
    10981 tagttaaaac tttagaagat gcaggggcat cagtcgttgc atatgaactt tgtggagcta
    11041 ttagatcaaa cgatttattg gttgatgaag aaatagaaga tgtatatgat gctttaacac
    11101 aaaaatatat aaatataggt tgttcttgta tgatgaataa tgacaatcgt attgaattat
    11161 tagatagaat aatagatgaa tataatgtag atgctgtaat agatgtagtt ttacaagcat
    11221 gtcatacatt taatatagaa agttatagaa taagagaatt tgttacaaaa gaaaaaaata
    11281 agccatttat gtctcttgaa actgattatt caaaatcaga tactgaacaa ttaagaacta
    11341 gatttgaagc gtttgtagag atgttgtagg aataaataaa agtaggagga actatgttta
    11401 gtataggagt tgactcaggg tcagtggcaa caaagggtgt gctatttgat ggtgaaaaaa
    11461 ttattaagaa aataattata ccaactggat ggagccctaa aagtacatct aaacaggtct
    11521 atgagttgct aagtagtgaa attgacaaaa aagatataaa aaaggtggta ggtacaggtt
    11581 atggaagggg agtaatggat tttgctgata agaaagtaac agaaataact tgtcatacta
    11641 gaggtattta ctttttaaac aataacataa ggactatttt agatgtaggt ggtcaagata
    11701 gtaaagttat aaatttagat agagatggaa atgtatttaa ctttataatg aatgataagt
    11761 gtgctgcagg aactggtcga tttttagaga ttacttctaa ccttttagga agcgatattg
    11821 aaagtataga tacattagct aaaggatatg aaccagttaa tatatctagt atgtgtacag
    11881 tttttgctga gtctgaaatt gtaagtcttc tagctcaaaa catttctact ggagaggtag
    11941 cagcagggat acttaaatct attgcaaata aatccacctc aatgttagca agaggtgagg
    12001 taatagatga agttgcattt actggagggc ttgcaaaaag taaagaacta gtaaaaatga
    12061 tagaagaaat actgggtaaa aaaattttta ttgcagaaga tactcaaata ataggagctt
    12121 taggagctgc tgtaatagga tttagataaa aacatataaa aagaaaatga gaagctgaat
    12181 ttattataaa taaattcagt tttttatttt attttattta tttataaagg aaaactttgg
    12241 tataattgat ttaaatgatt gtatatttgt aaatacttga tatttagtca tatataacat
    12301 tataatttta aaaattagct caaaataagg gggagaaagc gtgaaaaaat ttacttcaaa
    12361 aaaagtaaca aggtttgttg cgctgttttt aatatctatt ttagtaatgt caacaattcc
    12421 tgtatcagct gacaatagca atacattgaa tcagataaaa aaagcagaat acagtgagac
    12481 gtataaacaa tatctaaatg atgctaaaaa tggacgtaca gaaaaatata atgggattat
    12541 accaaatcca tatcagttgg aaggtactca aatccaaagt aaaggaagaa cagctccaac
    12601 atcttatgac cctagaaaat taggtcttat gacatcaata aaaaatcaag aggatttagg
    12661 aatctgttgg gattttgctg caatggcaac cttagaaagt ttcttaaagt taaataatta
    12721 tggagactat gatttatcag aagaacactt aagatggtgg gcttcagatg gtgaatatgg
    12781 atggtctgta aatgatatga atggtgcttt aaattatgaa gctatgggat atttaacatc
    12841 ttggtcaggt cctaaattag aaaaagatat cccatacaat ggtagagtat ctaaagcaca
    12901 aggagcaaaa aaaccaacta atatgaatac agcacctact gtttttaatg tgacagatgc
    12961 tgtgtgtgta agcaatgaca taaattctac aaaaaatgct atattacagt atggagctgt
    13021 aacatctggt tattatgaag atattaaata tcaaagtgat gaccaaaatt catattattg
    13081 tccaacaaaa agttttaata caaatcatgc tatttcaatt gtaggttggg atgataacta
    13141 ttctaaagat aattttaatg caaatattag accatcaaaa aatggtgctt ggttaataaa
    13201 aaatagctgg ggagattata attctgaggg tggatatttc tggatatctt atgaagacaa
    13261 gacacttatg tctgatatag ataatttttc aatcaaaggt ggtaaaaaac caaatgatga
    13321 tgagaagatg tatcaacatg actatgcaag tattgtacct ttaatttcaa aaaagataac
    13381 agctgcaaat gtatttgatt tcaatagagg cgatgaaact ctaaaatctg taatgttctt
    13441 aactgaaagt ataggagcta agtatgaagt atattatgct ccagtagtaa atggtatacc
    13501 tcaagaaaac aatatgaaaa aattaaaaga aggtactgcc caatattcag gatacataac
    13561 agttccaatt gattcttttg atataccaga aggaaagggt gcaattgtag tcagtataga
    13621 ggggaaaaat ggagagtcaa ctataggttc tgagtcaaat gtaccaggat atgatacatt
    13681 taaagcaaaa gctaatttag gtgaaagtta tatcatagat aatactggaa aattctttga
    13741 tataaataga gatagtaact tctatccgtg taattttact ataaaagctg ttacagaaaa
    13801 gtcttctggt gagagtatcc caaatgagtc tttaattggt agtgatagat atgaaactgc
    13861 tatcaaagtt agtcaaaatg gtttcaattc ttcagaaaat gtagttttag taaatggcag
    13921 ttcaatagta gatgcattgg cagcaactcc ttttacatct gctataaatt caccaatatt
    13981 attgacacaa aaagaagctt taaattcaaa aactaaagct gagatacaga gattaggtgc
    14041 taagaaagta tacctaatag gtggagaaaa ttctataagt aaagaaattg aacaacaatt
    14101 aaaaagctta aatatatcga tagagagaat atcaggaagt gatagataca agacaagttt
    14161 attattagct caaaagttaa atggtataaa aaatgtatct caaatagcag ttgtaaatgg
    14221 agtgaaagga ttagctgatg caattagtgt aggcgctgca gcagcagaaa ataacatacc
    14281 aatcatactt gcaaatgaaa agagcgaatt gcaaggagct gatgaatttc taaattcatt
    14341 aaatatagag aaatcttata ttattggagg aacagcatcc ctatcaaaca atctagaaag
    14401 taagttaaaa aatccaacta gattatctgg tagtagtaga gacgaaacta attcaaagat
    14461 aatagataat ttttataaaa aagatacttt aaaaaatgca tttgtagtta aaaatggaat
    14521 caagaatcaa aatgatttaa tagatggatt ggcagttggt cctttaggag caaaaactgg
    14581 gtcaccagta attttagttg gtgataaatt agctgatagc caaaaagaag ttcttaaaaa
    14641 taagacttta gaaaaagtaa ctcaagttgg tggaggagca aataaaaatg catttaaaga
    14701 gttagtaaaa ttaaaaagtg ctaaatagag tactt
//
LOCUS       DAELJO010000083        14610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELJO010000083 DAELJO010000000
VERSION     DAELJO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.083"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            21..983
                     /locus_tag="KPB88_002150"
     CDS             21..983
                     /locus_tag="KPB88_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054426.1"
                     /translation="MNQINSARNKIEKNNKIYNLLDIKPFKPTYETNKLEAYSTDSPI
                     SDDDSMDEYISNYKFDINLNEIILSLSINQGIYNSKKEHVTSFMISDNNEYIFCTKKA
                     IAYSIDWDKKSVRKKKLSEAKVNDWFLFVDDYLNEEGELVINVIQKLLELNLLPSHYL
                     DDYKLTNSWKSELKNYISYSKYNYNDVSNSLAVLGEKINPITIKNWVTNKRLIGPIYL
                     KTFEKIFKVIDSKLDHTEVYDATKSIRSLHTKIKKSIDTFVSDNFFSSKSDIIKDEVS
                     AVVLSMLGNTNDYVTPVQIREIHSCDKYLSPHLVNKVLDLYSVY"
     gene            1001..4528
                     /locus_tag="KPB88_002151"
     CDS             1001..4528
                     /locus_tag="KPB88_002151"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBG0054427.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            4530..6443
                     /locus_tag="KPB88_002152"
     CDS             4530..6443
                     /locus_tag="KPB88_002152"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HBG0054428.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(7149..7892)
                     /locus_tag="KPB88_002153"
     CDS             complement(7149..7892)
                     /locus_tag="KPB88_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054429.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(7914..8228)
                     /locus_tag="KPB88_002154"
     CDS             complement(7914..8228)
                     /locus_tag="KPB88_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054430.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(8232..8432)
                     /locus_tag="KPB88_002155"
     CDS             complement(8232..8432)
                     /locus_tag="KPB88_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054431.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            8579..9340
                     /locus_tag="KPB88_002156"
     CDS             8579..9340
                     /locus_tag="KPB88_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054432.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            9397..9595
                     /locus_tag="KPB88_002157"
                     /pseudo
     CDS             9397..9595
                     /locus_tag="KPB88_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9592..10083
                     /locus_tag="KPB88_002158"
     CDS             9592..10083
                     /locus_tag="KPB88_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054433.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            10175..10927
                     /locus_tag="KPB88_002159"
     CDS             10175..10927
                     /locus_tag="KPB88_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0054434.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            10917..12083
                     /locus_tag="KPB88_002160"
     CDS             10917..12083
                     /locus_tag="KPB88_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0054435.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(12199..12429)
                     /locus_tag="KPB88_002161"
     CDS             complement(12199..12429)
                     /locus_tag="KPB88_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054436.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(12435..12914)
                     /locus_tag="KPB88_002162"
     CDS             complement(12435..12914)
                     /locus_tag="KPB88_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG0054437.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWFDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(12914..13129)
                     /locus_tag="KPB88_002163"
     CDS             complement(12914..13129)
                     /locus_tag="KPB88_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG0054438.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(13461..13810)
                     /gene="ssrA"
                     /locus_tag="KPB88_002164"
     tmRNA           complement(13461..13810)
                     /gene="ssrA"
                     /locus_tag="KPB88_002164"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13968..14423)
                     /gene="smpB"
                     /locus_tag="KPB88_002165"
     CDS             complement(13968..14423)
                     /gene="smpB"
                     /locus_tag="KPB88_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG0054439.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         5404 a         1959 c         2053 g         5194 t
ORIGIN      
        1 tatccaaata taattactat atgaatcaaa taaattctgc aagaaataaa attgaaaaaa
       61 ataataaaat atataactta cttgatataa aaccttttaa accaacttat gaaaccaata
      121 aattagaagc ttattctacc gatagtccta tttctgatga tgattctatg gatgaatata
      181 tatctaatta taaatttgat ataaatttaa atgaaattat attatctcta tctataaatc
      241 aagggattta caactctaaa aaagaacatg ttacatcatt catgatttct gataataatg
      301 agtatatatt ctgtacaaaa aaggcaatag cctattcaat agattgggat aaaaaaagtg
      361 ttaggaaaaa gaaactttca gaggctaaag ttaatgactg gtttttattt gttgatgatt
      421 acttaaatga agagggtgaa ttagttatta atgttataca aaagctatta gaacttaatt
      481 tattgccatc tcattatctt gatgattata aattaactaa ttcatggaaa tcagaactta
      541 aaaactatat atcttatagt aaatataatt ataatgatgt atcaaacagt ttagctgttt
      601 taggtgaaaa gattaatcct ataacaatca aaaactgggt aacaaataaa agattaatag
      661 gtcctatata tttaaaaact ttcgaaaaga tatttaaagt tatagattct aaattagatc
      721 ataccgaggt atatgatgct actaaaagta tacgctcttt gcacactaaa ataaaaaaat
      781 ctatagatac atttgtatct gataatttct ttagcagtaa atcagatata attaaagatg
      841 aagtgtctgc agttgtttta agtatgcttg ggaatacaaa tgattatgtt acacctgtac
      901 aaatacgaga aatacacagt tgtgataagt atctgtcacc ccaccttgtt aacaaagttt
      961 tagacttata tagtgtttat taaataaata ggaggtaaat atgaattgta gtgaactaag
     1021 agataaaacc ctagatcttg taagaaaaga acttcttgga cctggttcgg aagatctttg
     1081 tgaaaatata aaatatgaaa taataacaga tagtccaata gagcgttatt caacaggtat
     1141 tttatatcca cttaataaca atattgatgt aagtaataca tctgaatatg atggtgaaga
     1201 taatgtattt acatcagatg atacacaaaa tgaactagat agagctataa gtatgtctaa
     1261 tgaaacttta ccttcttcta tgggtatgac ttttttccta aaaaaagatg cagaagtaat
     1321 aaatttcagg gtaaattatg ctacttatag aaatgcttta tatacagact gtcatgttaa
     1381 atatactggt gacgacttct attctagcaa tacaattaat gaatatgtag aacttagggg
     1441 atcattccta tatcttaagc aaaaactaac atctgagaag gtttataatt tactaaaaaa
     1501 gcatgattta cttgataatg ctgaagctaa atttgtttta tacaagttag ctaaccaatg
     1561 ccctgatgat gagaataaga ctggtggtta tgttcgtgtt ccacatgaaa acttaatagt
     1621 atcaatagat atggataaaa actatattaa aaaacctata tctaaagatt tagatatagt
     1681 tttatctaaa agaatttatt caaatgactt tgttagtatt acttgtatgc tggttaatag
     1741 tcaatctggg cctaagaaat ataataatac tattttccag cctgaactaa tggtattagc
     1801 tgaagataac aatgatttag agtttataga tatttttaca gctggatcag taaatacttt
     1861 taaaaagtta gattctgagg aaatatcttt agacttactt taccgaaata aaaaaacttt
     1921 tgctactggt catggtgtag ctatagatca agatattcaa aatgatgctt taataaaact
     1981 ttgtactgag tttattcctc attttgaagt acctaaattg gactttaatg tagatgaatt
     2041 aagtgatgtt tctagagaat tactgtctat gaaaaattta tcagatatct ctgatcttag
     2101 taaagataag aaaatatatt ttctttataa gtttgttgat gcttatgata tttggataga
     2161 tgatctagaa gaagattcaa aagatcttag tgatgttttt aaagatgtag catatgagca
     2221 tataaaaaac tgcagagata ctaagtctag aatgctagaa ggtataaatt taattaagtc
     2281 taatgatgaa gtatacagag cattttctct aatgaataga gctatactta tgcaaagatg
     2341 tcattctata catattacgg atagactccc tggatctaat aaccttgact atatagatta
     2401 ctcaaatgat aattctgctt ggagaccatt ccagcttgct tatatactta catgtttatc
     2461 ttcgatagaa aatcctgact ctaaacatag agatttagtt gaccttcttt ggatccctac
     2521 tggtggaggt aaaacggagg cctacttagg tctatctgct tttactatct tcttaagaag
     2581 aataagaaac aaagaaaatg gtaacggtac agctatcata atgagatata cactaaggct
     2641 attggctgca caacaattca taagagcaag tatattaatt tgtgcctgtg aaattattag
     2701 aaaagaaaat aagtctgaac ttgggaaaaa agagatttct attggtttgt ggattgggag
     2761 tgatcaaact ccaaacttta atgatgaggc agaagaaatg cttaataact taacaggtaa
     2821 taacattaag tttagatcta gtataaatag tgaatatgag ttagataatt taaaatcaaa
     2881 atataataaa ttccaattac taaaatgtcc ttggtgtgga acaaaacttg ttaaagaatg
     2941 ggataaaact tctaaaaaag ctgttggtga ttggggctat agaattaaat ctagacctaa
     3001 gaaattctac ttatgctgtc ctgaagaaga ttgttcattt gaagatatgt tacctataca
     3061 agttgtagat gaggagattt ataataaccc tccaacactt ttatttggta cagttgataa
     3121 atttgcaatg ataacttgga ctacagaagt agctagtctt tttgctttag atgatgataa
     3181 tagcaactta tctcctgagt taataattca agatgagctt catcttattt ctggaccttt
     3241 aggtactatg gtaggtttat atgaaactgc acttgatgct atgtgtagct ataagggtat
     3301 aaaacctaag attatagctt ctactgctac tataagaaaa gcacaaaatc aagttaaaaa
     3361 tctatataat cgtaatgtag ctcagtttcc acctccagga ttaacatcta gtaactcttt
     3421 ctttgttaaa gagatgcctt taagtaaaaa acgtggccga ctatatgtag gtgttattcc
     3481 atctggaaaa actcaagtta ctgtagaggt tagacttatg tcagcattac tccaaagact
     3541 taaaatgatg gagaatgtag agttgaattt attggataaa tattggacac tagtatctta
     3601 ttttaatagt atcagagaat taggtaagtg tagcacactt ttaaatgacg atataaaaga
     3661 caacatggaa agattatgta aaagagtctc agcccaccct tatcaatcat taaggaacct
     3721 aaatgaacaa agagagctta ctagtagact tacttctact aagataaata caactttaga
     3781 tgaactatct aatactctta cttctaataa tttaactgtc gatactctat tagctagtaa
     3841 tatgatttca gttggtgttg atgttgatag attaaattta atggtagtag ttggtcagcc
     3901 taagttaaca tctgaataca tacaagctag cagtagagtt ggtagaagta atgaaggact
     3961 tatatttaca ttatataatc ctacaaaaac aagagataga tctcattatg aacagttctt
     4021 tagttatcat caatcattct ataaatttgt tgaacctact agtattacac ctttctctga
     4081 accagctaga gaaagaggca tgcatgcagt attaataagt atgattagac atattttagg
     4141 acttcaagct aatgatcttg caagtcgatt ttcaaaaaat atggatggtg tagaagattt
     4201 aaaaaacttc ataatagata gatctatcga tatatttaga gaaaataatc aaaatgctga
     4261 tgataatata gtaaatgaat ttaaatctga gatttcatct gaattagata ttataatgga
     4321 tttatggcat gataaagctc aaatatctga taaagataat ttattgcatt attataagcg
     4381 ccctagtaaa tttgataata aaatatataa taatctttta attccttatg ataaggatgt
     4441 tattacaaat gaaaaacaaa cattaaattc tatgagaaat gttgataaac aatgtactgt
     4501 tgaaattata gaattggagg aaaattaata tgtctataaa attagatgat aaaactcata
     4561 atttaagaat aaatcaagct acaagtgttt ttggaccagg agctatgcta gattttattg
     4621 atcaaacatt aatgactgct catccaaatt attggactaa tacaattaaa atacaagatg
     4681 ataggcttca ggctacattg agggttaatg aactaagaac acctccaact atagatgata
     4741 gatcttctgt tccttttgtt aggtttccta gatggtactt ttgtccaaaa tgtagaaaat
     4801 ttatgcctat agaagattgg gagaaacttt ataaaaatgc tcataaagat aaacctatga
     4861 aaatacctat ttgccttact tgtagcacaa aaaagtctac taaacaatta gtacctactg
     4921 gtataattac tatttgtgaa catggtcata ttgatgattt tccatgggta aaatgggttc
     4981 attttagaaa taaatctgga agaaaagaaa tttgtgaaaa tcctcaaata ttaatgaaga
     5041 ctggaaaaag taacctagga ttagaaggta tagaacttga atgtagctgt ggagctaaag
     5101 ctaatatgaa gggcgcattt atgcgtttaa aatcttctaa atctaatgat tatggtgtac
     5161 ctagtgaata taaggaaatg tttaaatgct ctggtaattc gccttggtat aataaaaaag
     5221 taaataaatg cactaaatat tcatttgctt ctcaaagagg tgcctctaat gtatattatc
     5281 ctaagataga aagttcaatt attataccac catactctga tactcttaat tctaaagtgc
     5341 tatctagtaa aggttatgat acatttaaga aattcttaga tcgtgctatt aaaaagaata
     5401 agttagaacg ctttatggat gaggattttg aggactttgc cagtgatatt gctaaagaaa
     5461 caggttttga tactgataaa gtatccatag ttttgaaaga tttattgcct tttgaagatt
     5521 cttcaccatc aacaacaact agaaatcaat atagagaaga agaatatgag gctttaattg
     5581 gaaatataga tgctaaatct aaaaaaacta atgactttac aatagaagaa caaaatctat
     5641 ctgattatga tattccaaac ttcagtaaaa tagtgttagt aaaaagactt agagaagtaa
     5701 gggcattaac tgggtttagt agagtcaatc cacctgataa taatatattt ggtgaagaag
     5761 atatagatga tagctgtaat gatactaagg ttataagttt aagatctaga gattgtaaaa
     5821 aatcttttta tcctgcatct gaggttagag gtgaaggtat tttcatagag ataaataatg
     5881 atatggtaaa aagctggatt tcatctaatc ctaaaataca ggataatgca tctattttaa
     5941 attctagata taataaagat attaatgtta atgacaaagc ttatagaaaa attacaccta
     6001 aattcttatt actacatact ttagctcatt taattataag agaacttagt tttgtttgtg
     6061 gatatgctac ctcttcactt agggaacgta tatattgtaa taattccgaa aatgaaaaag
     6121 ttatgtctgg aattttagtt tacactgcaa gtggtgactc tgaaggtact ttaggaggat
     6181 tagttaggca aggtatgcat gattcattac catctatatt aaatagtgct cttaatcgtg
     6241 ctctttggtg ttctaatgac ccaatatgta ttgatagtac tggtcaaggt agaaattctt
     6301 taaatctagg agcatgccat tcatgtactt tattgcctga aactagctgt gaggagtata
     6361 atttattact tgatagaagc atgttaattg gtgatatgga taatagagat attgggttct
     6421 ttaaaaatct aattttaatt tagtaataac taatttttta tttatataac taaaggttaa
     6481 aagttaattt tatgacttct atcctttagt tttttgtatt taaattatta ttttttgcaa
     6541 atcattctta ctagaaatta cttattataa ctaattccca ataattatat aattttgtgc
     6601 tcatactgaa atttttaaat aaactattaa tttatattat atatgatttt tcaaaataaa
     6661 aaacatatta actattaaat caatatgttt tcgtggattc caatggtgga gaccgaggag
     6721 aatttataag tggttatttg attgttttgt tggtttttat aagtataggt atatggctat
     6781 ttggtcatga tgttgaagca atttttctgg ttatttttta ttgtctattt ttgtagttgg
     6841 ggtattattg gggtataatt ttctactttc caaaattatt gatacttcaa taatataaat
     6901 taaattctta gttaagtttt ttaatgaata ggtaaatatt atttgtactc ataacaatat
     6961 agtttacgca taattttata tatattttct ggtatatctg caactaactt agtgtaattt
     7021 aaacgatatt agagatactt aaaaaacaaa tatatgaaag atttataact aacttagtgt
     7081 aatttaattg attgctctgc ttctgtattt ttaattaagt tgtttgaatt atttataata
     7141 taacctcctt accactttaa ttgtgagtca tctggattat atattcctgt ctctttattt
     7201 atgacaattt ccttgctatt ataatttccc tctagtatct tagtaaaatt acttgaattc
     7261 attatccaat caaaatttgc tttccatcct gtattattat ttccattcag aaaacttgtt
     7321 ttactaacta tatttataac ctctaataca gtttctttat catattcttt tattcttgaa
     7381 tttattctcc tctttcttgt atctgttagt ttcttgacct ttgaaagttc aatacaagtt
     7441 gagttaaatt tgcttatgat ttcactatat ataatattat tttcattctt tatattcttg
     7501 attgttgttg actgattgtt aatagtctgt tgattaggtt gttgattttc tatattctct
     7561 tgataaagct cccagttttc aatagttatt actatatact tgtttgttgt ttcgtatgtt
     7621 agaaaaccag catttttaaa aaccaacaaa caacttctaa ccatttgata actaactcct
     7681 ttaccacatc tttcagctat tttgctatat gaagaaataa attgtccaaa ctttattttt
     7741 atctttttat tatctataaa aacatgattt tctttatgac tagccataag taatagagtt
     7801 attaatatta cctttctttc tggtttaata tctttccaaa ttgatttatc caacaactcc
     7861 ctataaagct ttatccaccc tttattgctc aattgcaaac acctacttta agactataac
     7921 ttgataccat ttgcattttc aataaaccaa tcatctaatt tattttttat tatgtatact
     7981 cgattattta tccatataca agggaatcct ttgtttttag aatgacataa ctgcctaaat
     8041 tttgattctc ctataggata aagcttagat gcttctttac atgttataag ctcagcttta
     8101 tttcttttcg cattttcaat aattttatat ccttgctttt tttcttgtgc ttcttcttca
     8161 ttctttacaa taatgtttaa agtggcttgt aatgtcttta aaacctctaa attattattc
     8221 tctttcatat tttaatcctc cttactaaaa aataattctt ctactgttgt atctaaagct
     8281 ctagatagtt taatcatcaa atcattgctt ggattattag ccttacctgt ttccaagttt
     8341 gataaataat atcttgatat accaactcta tctgccagtt cttgttgctt tagacctttt
     8401 ttaatcctaa atatttttaa attgattccc atactttttc ctcctttttt ctagtttaca
     8461 ttcgtaaact ttacatttta attataatgt cgatatatgt gtactgttaa taagaatata
     8521 ttatttttct gttctcattt gtgtactttt gggatatact ttatacgagg tgataatatt
     8581 gcaaattgga aaaaacttaa aaaagataag aaaacaaaaa gagctaacgc agattcaact
     8641 tgctgaaatt agtggcatct caagaaatgc tctaataaat tatgaaaatg ataaaagaat
     8701 acctagtata gataccttat caaaactcgc taaagctttg aaaattgaaa agactatctt
     8761 ttattttgat attgaaaatg aagtaatgac taatgaagat gttttatacc ttttacagtc
     8821 tttattccca gaagaatact attcttttga tgaattttta aatgctattt caaatcttat
     8881 ggaagatgat attagaatta cctcaagttt attgctaggt gcctggtctg ataaagacca
     8941 atattataaa cttggtgaaa aattagaact aaatgaaaat caagtttata attggatact
     9001 ttcagattgt atatctaata ctgaattaga tttttcatta catcctgatg acataaaaga
     9061 aattattaaa aatgatgttc ttacaaaaca atcaattgat gaattattag atattggtct
     9121 atcagaagaa agtatagaaa caatctatga ttactttagt gactcaacag ataatggaag
     9181 taatattcga ctaaaaaaat taatatctca aaaagaaaat attactatta aaaataatga
     9241 ttactttaaa ttattacaag aaaataacga actaaaggaa aaaaatgaaa agcttgaaaa
     9301 tacacttaaa gaaataaaaa aactactaac taaaatataa aataatatca atactatcta
     9361 aagcaatgat aaaaaactat gaatggagtt acaattatga ataaatcata tatgctaaaa
     9421 tacacatata aatcccctac taatttattt catggagata aaaaatatac tctaacatac
     9481 actaagagag ttatgagtaa tttagtatat gatatgggat aatctagaaa ataactcctt
     9541 tacatgttta gaagtacaat cattgtttga tgatatattt aaggtgaaat tatgatgaat
     9601 aatacaattg gagaaaagat taaacaatat agaaaatcga taaatatgtc aagggatgag
     9661 ctagccaaca agattggttg ttcagttcat gctatagcta aatatgagca aaatcaaaga
     9721 atgcctagtt tagatatgat aagaaaaata gctactgctt tatcaattgc tataaataaa
     9781 ctcattgttg aagaaaatga tttagatact ttaacaaata ataatattaa tttaaaagta
     9841 tttgataaag agttggattt agtaattcac atattagaaa attatggtta tgatataaaa
     9901 acagactata aaaaaaagtt tgaaattaca gataatgcag atacaataaa accaattaac
     9961 ctaaacttct ctgacttcaa agacttctcc aaaaatctaa attgggtgat aaatggtctt
    10021 gttgaaaatt ttatacaaga aaactctaat ggtcttattg aagatgagga tgaagaatat
    10081 tagtattgca cattttcaaa atatgagttt acctattaac ttttatacat tcatagttaa
    10141 taaataaaaa ttagatatga aaggagctaa aatcatgaat aaatcatata taccaaaata
    10201 cacacataaa ttccctacta atatacctca tggagataaa aaatatactt taacatacat
    10261 tagaagaatg ataagtgaat ttgtatatga tatgggtaac ctagaaaata accccttcac
    10321 attcccagaa gtacaaacat tacttgatgg tataaccgta ggcggacata aattaagtga
    10381 ccaaaatcaa atactaaata taaaatcatc atgggattat gtaataaaat taagtaacaa
    10441 agaaaactta tctttaaata aagatacaat ttgctctata cataaaatag ttgcaaaaga
    10501 tgaagctcta atcgttggag atttcagaaa tggtaatata ggtatagctg gtactactca
    10561 atatgaatgc atagaagcca ctctattaaa tgatttattt gtaaatgaca taaatatcat
    10621 aaataaaata actaacccat tagaaaaggc tataataata aatttatggt tgtcctattg
    10681 ccaattcttc tatgatggca acaaaagaac tgctagactt acctcaaacc taatacttat
    10741 aaataatgat ataggagttc tatcaatacc agctaaacat aaacaagagt acaacacatt
    10801 aatgctaaac ttctatgaaa cactagaagc tgatgaagtt ataaaattct tattagagaa
    10861 atgtattaca ttctttgatg gatataacta taaatcatat aaggagctgt ttaaaaatgg
    10921 caactaaaag agctaatgga gaaggctcta tcggaaaata taaagatggt tggcgttccc
    10981 gtataatgat tggctataat gaagatggta aaccactgag aaaagaattt tatggtaaga
    11041 ctcaaaagga agttaaagat aaattagatg cttataaaaa acaatattat ttaagctctg
    11101 atatttcaga tgataaaata actttagagc attggtttta caattggtta tttgaatata
    11161 gaataaaaga tttaaaaccc aagtcatttg aaagatatga aagcatctat cgtaattata
    11221 ttaaagatac tgatttaggc aatgttaaat taaagtactt aagagtatct cacatacaaa
    11281 aatattacaa tagattgctt gattccaata aaagtatacc tactataagg caaataaaca
    11341 ctaaactgaa aacctgctta tcagaagctg aaaaacaagg tcttatacaa cgtaactatt
    11401 gtactatggt aaatatacca gttgagaaaa aagaaaacaa acttgaaata ttgactcttg
    11461 aacaacaaaa gagttttata aaagctatag atgggcataa attagaggta ttatttttac
    11521 ttgctcttgg tactggactt agattaggag aattattagg acttaaatgg tttgatattg
    11581 attttaaaaa gtctaattta acagttaaaa gaactcttca aagaacatat tttatagata
    11641 aaactggaaa tagagagctt aaagtattag agcaagggcc aaagacatca aattcttaca
    11701 gaactgtacc tattccaaaa gatgttttaa ataaattgaa agagcataaa gtaaatcaaa
    11761 atacagatat attaaaggct ggagaattat atataaataa taattatgta ttttgcaatg
    11821 aattaggcat ccctatagat gataaaagac ctttaaggaa tttaaagtct atattaaata
    11881 gcttaaatat agagcctata aagtttcatg gtcttaggaa aacttatgct actagacttt
    11941 ttgagaatga tgttcctcct aaaactgtac aagttttaat gggtcattat gatatatcaa
    12001 taactttgaa tatatatact caagttatgg aagataagaa agttgaagct gtagaaaagt
    12061 tggataaaat attttcattg taaaaatcaa atacatataa atattgtgat ttcatatagt
    12121 taattacaaa atcaaaataa ggatacaaat taattttata ttatattgta tccttatttc
    12181 gtatctagtt ttaattattt atcttggata gcccttatgg tacattaatc cattttctaa
    12241 cattaattct tgagccaagt atatatgacc cttacagtgc atatctgata caccctcact
    12301 tataagttta tatgtttcag aagtatataa ataatccata ctttcttcca agctcttacc
    12361 tgtttgtttg gaaaattcta atatgatttt tttatatagt atttgtaata atgttttatt
    12421 tgcttccata atcattatac ctcctcttca tacctaaact ttaaatattt atctattaca
    12481 ttttgattgg ttatacatat ttgttcattt ggtttatagt attttaatct ttttaaagct
    12541 tcatcttttt caataagatt atcttgatac agttctattg tattgtaaat cttatcatca
    12601 gcaataccac ctattataat gtcatatttc atatatatat cactcatatt tctacattct
    12661 attataaaat caaaccattc taaattataa ctatcgaaga tttttacctt gcagttttct
    12721 tttaatttat caatatctaa agaataacta tttaaatatg cttttttacc tcttttttta
    12781 aaacgctctg tccagttaat tgcttgtgtc tttatactgg ttacatagaa tccttttcca
    12841 aaatctaaag aatctcttga aaaggtaata ctaggcgtct ttaccacaca atatgaacca
    12901 tgatatacaa tcattatatc acaccctttt ctttaagtac ttgcattaaa tcttccacta
    12961 tgtaaccttt actttgagaa tgtagtgctt catagcaagg tattatatag ttatctaaaa
    13021 tatcagtatc atgtttaaga agtttataaa ctccttctga atctatattc aatttttctg
    13081 ctaaagcttc aatgcaaaaa atagaaaatt ctagttttct ttcatccatt acaaacctca
    13141 actcctatca agcttatata atatatattt ttactaatta acaacttaaa ctgtcttaca
    13201 tttataaaaa tattatatca taaagtagat attaaaattc tatgttacct attatcataa
    13261 taaacaaata aaataataaa caaataaaat aataaacaaa taaaaacttt ttatacagcc
    13321 taaatgcacc ccaatatacc cccaaaaaaa cctcattttc tgtggttggg gtacggttgg
    13381 tggatttctt ctccaaaata aaaaatacta gtcaacaaag actagtattt ccaagtattt
    13441 catcattctt ttttcaaaat tggtggagac gaggggagtc gaacccctgt ccgcaaatct
    13501 tttccttgct gcatctacat gcttagccaa cttcactaat atctcacctc acatagcgcc
    13561 aagtggcagg ctctatgctt agccagtccc aattaatcta cttcaagtcg agacacccct
    13621 aaagtagttc cctgcataat gacgtccagt cttagtcggc aggaggacta agagggacga
    13681 gcagcaacta ggctgctatc gcgaaattat cgtctgcgtt tattttaaag ttccccagtt
    13741 tttacgtgac ccagagtaac acgacatgca gcctcaagtt ccaaacccac gtcgaaacct
    13801 ttacgccccc ttgagtagtt tatagtccag tagacttttc tcaaacttta tcaccatttt
    13861 tattaaattt ttaatgtata tataatataa tacttttaag aataaaaatc aatgttatgt
    13921 tctagataat aaagaatttt attctaaaaa atacagtttt atctagttta atacttacct
    13981 gtcatttctc tttctatacg tctttgtgca tctttcttag ccatatcttg acgtttatca
    14041 tatagttttt taccttttgc taaggcaatc tctactttaa ctttaccgtt ttttagatac
    14101 aaactaagtg gtataagtga ataccccttt atagtagttg ctccaataag ctttcttatt
    14161 tcatgcttat gaagtaatag ttttctaact cttaaagaat caacattgaa tatatttcct
    14221 tgctcatatg gacttatatg aacttgtttt aaaaatactt cgctattatc tacagatgca
    14281 aatccatcac ttaggttaac tttgccttgt cttattgatt ttacttctgt tccttttaat
    14341 tcaattccac attcataagt ttcttctata aaatattcat gtcttgctct tttgtttgtt
    14401 gctaatattt ttttaccagc catataaatc tcaccttctt tgctagaaat ttgttccata
    14461 aatgttagaa atttactaaa tatttaatgt tgccaaatta tttgactaac ttgtatgata
    14521 acatatttag gcattttagt caatatttca ctgatatatt aagttagtta ggctatatat
    14581 aattaagtaa tataaatgta tgaaaatagc
//
LOCUS       DAELJO010000084        14423 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELJO010000084 DAELJO010000000
VERSION     DAELJO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.084"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..1874)
                     /gene="recJ"
                     /locus_tag="KPB88_002166"
     CDS             complement(<1..1874)
                     /gene="recJ"
                     /locus_tag="KPB88_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0054440.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     G"
     gene            complement(1887..3551)
                     /locus_tag="KPB88_002167"
     CDS             complement(1887..3551)
                     /locus_tag="KPB88_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG0054441.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3849..4346)
                     /locus_tag="KPB88_002168"
     CDS             complement(3849..4346)
                     /locus_tag="KPB88_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG0054442.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4375..5754)
                     /gene="scfB"
                     /locus_tag="KPB88_002169"
     CDS             complement(4375..5754)
                     /gene="scfB"
                     /locus_tag="KPB88_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG0054443.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPSGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6059..6205)
                     /locus_tag="KPB88_002170"
     CDS             complement(6059..6205)
                     /locus_tag="KPB88_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0054444.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6235..6813)
                     /locus_tag="KPB88_002171"
     CDS             complement(6235..6813)
                     /locus_tag="KPB88_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0054445.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7181..8611)
                     /locus_tag="KPB88_002172"
     CDS             complement(7181..8611)
                     /locus_tag="KPB88_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0054446.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            8955..9620
                     /locus_tag="KPB88_002173"
     CDS             8955..9620
                     /locus_tag="KPB88_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054447.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(10126..12828)
                     /locus_tag="KPB88_002174"
     CDS             complement(10126..12828)
                     /locus_tag="KPB88_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0054448.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(12854..14404)
                     /locus_tag="KPB88_002175"
     CDS             complement(12854..14404)
                     /locus_tag="KPB88_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0054449.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
BASE COUNT         4795 a         2501 c         1366 g         5761 t
ORIGIN      
        1 ccatttatag aattagatat tattagtgta tctttttcaa aataatcaaa tatatttata
       61 tctgtatttc catcaaataa ttcaaaatca gacacagata tattatacag gctttgagta
      121 tcttttggta ttatttttga cattaatttc aatgaaagtt tgtcattact tgcactttta
      181 ggtcgagcca atcgtatatc tttaagtaaa aattgtactt tcctatttcc catatagtta
      241 ttttcatcta attggaatag tacatctacc ttgtctccta ctttatacat tgattttaaa
      301 tgactcatat taaatcctat acattcatag cttttctctt tttctatact taatttcaga
      361 tgttgttgat tcttacctat aactttcaaa tcttttaaaa tataatttct aactataaat
      421 ctaggattag gattatttaa tccaaagggt tctaacttat gtaattcctc tacaagattt
      481 aaatttatta cattttcttg taattcaaac tcgacattta cattctctat catatcatct
      541 tcagttaaat tataatctgc aaatttattt atttcatttg ctaaaatttc tacattgtct
      601 gaatccaagg ataaccctgc tgcttgttca tgaccaccaa acttagtcat caagtctttg
      661 cactttatga gagcttcaaa tatattaaat ccttttattg acctcgctga accagtagcc
      721 tccccatttt ctatacctag aagaattgtt ggtttgtagt atttttcagt taactttgat
      781 gctacaattc ctattattcc atgttgccaa ccctctttag ctaaaaccaa cactttgtca
      841 tcattgtatc tgctattaga ctttagcatt tcttcagctt catgatacat cttagcttct
      901 atcatctgtc tttcattatt tttttcttcc aatatactag ctatttctac tgcctcttct
      961 tgtgattgag tagtaaatag ctctactcct aagtaagaat atcccaatcg acctgaagca
     1021 tttatcctag gacctattgc aaaacctata tgagaagaac ctatcttatc actttctaca
     1081 ccacaaactt ttataagctc ccttaatcca atatttttgc cttctttcat tgattttaaa
     1141 ccatttttta caattattct attttcatct ataagtggaa caatatcaca aatggttgct
     1201 agtgttacta tttccaaata attatacata ctagttttaa actcttcttt tggagtcaag
     1261 gcttgaatca ttttaaatgc tacaccacat ccacatagtg aatcaaatgg ataattacaa
     1321 tcttcttgtt ttggatttat tactgcaaag gctgatggaa tttcactttg acattcatga
     1381 tggtctgtaa ttataacatc tatgcccaaa tcattcgctg tattgacctc agacactgat
     1441 gtaattccac agtcaacact tataatcaag tcacatcccc tagagcttat aagcttaata
     1501 gcatcttcat ttataccata cccttcttct aatctattag gaatatagta atcaacattg
     1561 taacctatac tcttaaaata taagcacaag atagatgtag atgatacacc atcaacatca
     1621 taatctccgt aaatccaaat tttttcgttc ttctcaactg ctaactttat tctatcaaca
     1681 gctttttgca tatctttcat taaaaaaggg tctcttaaat aatctaaaga tggattcata
     1741 aatatttctg aatctttttc agtacatatc cctctatttt ttaaaatttg gcagatttcc
     1801 ggggaaatat taagtttttt gctaaattca ctttcctcta tcttaccttt atacttcaat
     1861 atccattttt tcaaatttaa ttccccctaa tcttttttgt attttgacct aatacttctt
     1921 ataactaagc aaaggcctat tataaaggat ataaagaaac ccgctattgc agtaaatttt
     1981 atccatatat tattttctat atttggagat gcaattataa gagaagaacc tactataata
     2041 gatgccaaca ctaaactcaa agatatttga gatgttagct ctattataca attttctaac
     2101 ctcgtcattt taacatcttc aatttgcatt tttatattat ttctttctat attttttaaa
     2161 attgctttta attgctttgg aatagttttt atgtccagta atatttcttc tacattttgt
     2221 cttgaactta aaacaacatt tttagggtta aatttactct tataatgatg tttcataaac
     2281 tcttttccca tagaaccaaa agaaaaatct gtgtttaaag ttctacttgt tccttctaat
     2341 gttataactg tctttgcaag agttacaaat tgtgcaggca tgactatttt atactgtcta
     2401 aaaaacctaa atagttcatt taatatatct gttacattta ttttttctat agaaatatca
     2461 tagtaataat gtattagata aagtaaatcc tgtctaagtc ccttaatatc agcctctcca
     2521 tttaatgcat ccatttctat caatagatat attatcttgt caacattctt ttctacacct
     2581 gctaatgcaa tttcatttaa ttggtttaaa gttttattat caataattcc aatcattcca
     2641 aaatctatat atgctatctt atttttagca actacgaata tattacctgg atgtgggtct
     2701 gcatgaaaaa atccatgaga taatacctgt gtaaaaaatg acctaacgcc tatttcaaca
     2761 atagtttttg tgttataacc tagtctcctt attttttcta catcacttaa ctttgtacca
     2821 ttaacttttt ccattactaa aattttctta gtattatatt ctgaatatac tttaggtata
     2881 tatacttcat cactattttt aaatatccta gagaatttag ttgcatttat agcttcaaat
     2941 gtataatcaa gttccctcat taattgagtt tgaaattctt caatcatctg tactaaatct
     3001 aaattaaaat ccttttttaa atcttttaaa gtatttgcaa tagttctaag aatctctaaa
     3061 tcagatttta ttattttctc tatatttggt ctttgaattt ttacgattac ttcttctccc
     3121 gttttaagta caccttcata aacttgacca attgaagctg ctgctattgg ttcttcttta
     3181 aattctaaga atatttcttc tatacttaaa cccacttctt ctttaaatat atcaatagct
     3241 atatttgaat caaatttctc tacatcatct ctgagttttg atatttcatc aattatatcc
     3301 tggtctaaca agtcttttct tgtactcaat atctgtccta tttttatata tgtaggtcct
     3361 agttcttcta aaactctttt cattctctca ccagttgtca tattttttat ttcttctggt
     3421 gggtcaaata gtggtatttt ataggcaatt ccttctatat ttagtctttc tacaataaaa
     3481 ctaaaaccat actttattaa aacatatcca atttctctat atcgctttaa atttctataa
     3541 cttattctca aatcttttca ctcctaagat aggtataccc aaaaacttac accttaattt
     3601 atcgtatatg acattatttc accaattgat attctaatat cctatatcct ctttaatatt
     3661 taattttatc acatatacta ccaattttat taaaattatt taactcagtt aatttttatt
     3721 tttgacattt tatatctggt ttaatcaaat actttttgaa aactaaaata tcaacaaaat
     3781 ctaataaaat aaattagtgg ttgcctcaaa acagatttaa atatgttttg aaacaaccac
     3841 tctttaaact aatatttttg attttgtgct gcctctaaat accattcata agattcatca
     3901 atatattttt tatcttcttc tgtaagctct ctaacaacct taccaggaga acccattatc
     3961 aaaacacctg gtggaaactt cttattagat gtaattagtg tacctgctcc tatcagagta
     4021 tattctccta tttcagcatt atccaatatt attgaaccca taccaattaa cacattatca
     4081 ctaattttac aaccatgaac tatagaccta tgcccaactg ttacattgtt accaattata
     4141 gtttctgtat ctccatgcac tatagaacaa tcttgtatat tagtgttttc tccaatagtt
     4201 attggtcctt catcacccct aactactgca ttgtaccata tacttgaatc tttgcctatt
     4261 ttaacatttc caattacatc tgctgatttt gccacaaata cactttcgtc aattaaaggt
     4321 ttatcttcta aataatctct aatcatatat aaacaactcc tttaaaatat aaatttagtt
     4381 ggttgctcct tctaacatta acttagcttg tatcattata gagcactctg tacgttttct
     4441 ttgcatttca cagcctaatt catatggttt tgatatgtca ttattaaagt ttatcgcatt
     4501 tgcatgacat ccacctgaac agtaaaactt attccaacat tgtttacact cttctttagt
     4561 atatacatga gcttctctaa acatattttt taaattttct ggaagtacta tttcttcatc
     4621 aaatatattt gccattttaa attcttcatt tccaacaaat tgatgacatg gatagatatc
     4681 tccacttgga gttacagaaa gatactcatt tccagctcca catccagtta ttctctttat
     4741 tacacatgga ccttgattta ggtctatcat aaaatggaaa aatttaaatc catctccttg
     4801 taattgtcta tctgcatact ctacagcaaa tttttcgtac tcttcaaata tcttaggtaa
     4861 atcttcttct ctaagtgcat atggattact ctcatctcca acgactggct ctacagaggt
     4921 taatttaaat cctaaatctg caaaatgcat aacatctttt gaaaaatcta aattatctct
     4981 tgtaaaagta cctcttatat aataatactt atcttttgct cttttttcca caagtttctt
     5041 aaatcgtggt aatgtaatat cataacttcc tttatcattt aaagttggtc tcatattatc
     5101 attaacttct tttcttccat ctaaacttaa aactacatta tgcatatttt catttatata
     5161 atctataatc tcatcattta gcagtacacc atttgtagtt attgtaaatc ttatattttt
     5221 attataatct ttttctacac ttcttccata gtcaaccagt tgttttacaa cttcaaagtt
     5281 catcaaaggc tctccaccaa agaaatctat ctctaggttc tttctacttc ctgaattagc
     5341 aatcaaatag tcaattgctg ccttaccaac ttcaaaactc ataagttctt tttctcctcc
     5401 aaaatcacct tgagctgcaa aacagtattt acattttaag ttacaatcat gagctacatt
     5461 taaacataaa gcttttacta caggctctct atgtacaaag cttggatgat attgatatgt
     5521 atcttctgta tataaaagac cctctttttc caaatttaat atttcttcat atgcttcatt
     5581 tattttttct tcttgatatt ttgattttaa tatctctaat atttcttctt tacttttttc
     5641 tttataaaaa tctaacaaat cgtatgcaac atcatctaac acatgaacag ctccaccatt
     5701 cacatctaaa acgatattat atccattcat agaaaattta tgtatcatac tcatacccta
     5761 cctcctaaat tactatctat actaaagttt tttattattt taattaaact ttacccttca
     5821 ttatatcata agtttacata aaatacatac aatacaaaaa tgacttactc tccgtaagtc
     5881 atttcattaa atacactata tccccctaaa ttggcgcact aatatattac caaataggta
     5941 ttaggctcct ataaaataaa taaaataaat tttgtatctg attttatatt gttttaatct
     6001 tattaaaata taataatttc ttttttcctg ataattttgt ataaataagt gcttgtattt
     6061 aatttgtctt ctttagattg ctgatttctt tgggtacttt aggctgatga ataatccaag
     6121 gacaggtact attagctgac aaaatagcag tgctaagtgc caatgagcta gcaaacttca
     6181 taaatcttac aataaacttt ttcataactt aactctccct catacatatt ttacttatac
     6241 tttagctttt aacttaccta atactaacat cacaaaaatc caaaacatgg caaatgcgct
     6301 atatgtgaaa taatctacaa atatactcaa agaaagactt aaaattgtta tcaataacac
     6361 tactgtagat ataaatatag cagttttctt ataaaccaat ttttccctat cacttatagg
     6421 gttactccta tgttctattg ggcacagaac acaaattcca acccaactaa cacttgcaat
     6481 aatcataata atatttttaa ataagtctat atttatattt tctataatta agattgtaga
     6541 taaaattata aaaataaagg ttaataagca tctaaaataa ttgtctgcat gataccctcc
     6601 tgaaaattgt cttattggac aataacacat taaaaaaaat aatacatatg taaatttacc
     6661 aaataaaaaa cctataaaca gcatggtggt tatattaact atgaaagcta ttaatatttc
     6721 aaatccataa gaatagactt tagattcatt attatctatc atattattac aaattaaaac
     6781 agatgtcatt ttttcagcat atcttttaaa catttttacc tccaaaaaaa ttttacatat
     6841 atagagtaca ttcttttttg agaaaattca atatacttta caaaactagt tgttaaaaat
     6901 gtaaaactgg tcaaaaaaag acctatataa ttttcttctt attatttata taacaaaaca
     6961 attataataa aacaatttta tcaagcttta tatgcataaa aagcctatgt caaaatagaa
     7021 ataaaccttt tccccattgt taaaaggcta aaaatatatt ttatttcata taaaaagagt
     7081 acctcacaaa ctaaaaagtt cattttgaga tacccatttt atttattaca aaatatcaaa
     7141 ttttatttat ttgcatatat tttctatata ttttagcctt ttaactttgt ttttgtttta
     7201 catactgtag tgcttttcta ttctgtttta ctgcttcaag acaaactttt tctgtttgat
     7261 tatcaacaaa ttctaaagct ctataatttt gctttactgc ttctaaacat attttttctg
     7321 tttgattatt tacatatttc aacattctat aatcttgttt tactgctttt agacaaactt
     7381 cttctgtttg ttctttcaca tatttaagcg cttcataact ttgattaact gcttctaaac
     7441 aaatctcttc tgtttgttcc tttacataag ctaaagcatt atagttttct cttactgcct
     7501 ctaaacatat taattctgtc tgattttcta catatttaag aatttttcca ttttgcttta
     7561 ctgcttctac aaatattctt tctgttggta tctttacata acttaatatt tcttttattt
     7621 caagttcttc aaatgaacct cttactgctt ctatacatat attttcagtc tgttttttta
     7681 catattgcaa tacttttcca ttttgtctaa ctgactccaa gcaaatttct tctgtttgct
     7741 cttttacgta ctgtaatgct cttccatcaa ttcttactgc ttccaaacaa atctcttctg
     7801 tttgcttatt tacatactgt agagccatat aacttctttt tactgcttcc atacatattt
     7861 tttctgtctg ttcatttaca taatacaatg caaagtcatt ctgttttact gcttctatac
     7921 atatttcttc agtttgctct tttacacaag atagtgcact gtagttttct tttacagctt
     7981 ctaaacatat actattactt tgttccttta cataagctaa agattttcca tcttttttta
     8041 cagcttctaa acatatggct ggagtttgct cttttattcc ttttaatatc tctgcaacat
     8101 catttttaga tgccctttct ataaatgcta gacatacatc ttctgtttgc tcttccacaa
     8161 atttaaatac ttccatgaca tcataagttg atgcattttt tatagcctca attagtacat
     8221 tttctgtctt attatttatg tagtacaaag ctctgtagtt ctgttttact gcttctatac
     8281 aaatatcctc attttgattt gttatatact taagagattt atatgtctgt tttactgctt
     8341 ccatacatac tttataagtc ttgtccttta caaactcaat tatagtacca tcctgcttta
     8401 ctgctagcag acacatctct tctgtttggt tttctatgtc ttttaactct ctgtaatcat
     8461 attttactct gcttaaatca tttaaatgta ctttgttatc ttcctttaaa tcttcttcat
     8521 tattttctat atcatactca aattcttcat catctaaata gtcatttttt tctggtaaaa
     8581 atatagattt aattttattc tttatatcca taaaattatc ctcctttaat atgattctat
     8641 cattaaagta aatcgttttt aagcacttat ttaattaatt ttatattaag tttatttaaa
     8701 agtctattca ttgtcgatag attatatttt atattaataa ttttatctta tatatgtggg
     8761 gtaataaagt taatagtgga cattttccat cccattagta tacactattt atatttatta
     8821 ctcatttata gtgatttttt tcaaattttt tcttatttac tatatcatat ttattcaatt
     8881 tatgatatta tttaaatata atataagttt tataaagtat attaaatata ttataagatg
     8941 gaggtcgagt aattttgaaa ggaaacatag tacaatataa ttttgcagat atagaggaag
     9001 aagtatattc gttggattat gctatagcat ggaatacaga cgaggaaaac gtaaacataa
     9061 taccatttac aaataaattc tgtaaagaat ctatagaatc tttttgccta ggtaaaatta
     9121 ataactttgt tgaaatatta aatgaaggtt ttgttgaaaa tcatcattat gtacatttag
     9181 ataaaatgat tagtgttcct aagaaaaagg ttaatttagt atatcaacaa gacacacatg
     9241 gttatcttct aagagatgat aatgacaacc ttattcctgc aaaaataact tcagagcaat
     9301 ctaaatctat atcaagtaag atggagttat tctgtgctgg tgaagaaaaa tgtttaatta
     9361 atatattact aaaggctgac ccatcatata ttttagatgt agatagtata aaagacaaaa
     9421 acattctaaa tctaggttat gagtctattg acagatataa agaatacaat tttgatgatg
     9481 ataagatttt aatatttttt ataaataaaa aacgttattc agtgattatg aaaaaaacaa
     9541 acaactctga taacgattta gtttccagaa acaatgctat taaagaatta tttactaata
     9601 aagcaggaaa tcttaactaa taatataatt ttaaattatg ttattgatta aattaataat
     9661 agtatacaaa gaggctgtct caaaatagag ataagcttct ttttcattgt tgaaataata
     9721 aaatatattt aattctatat aaaagagagt gcctcataaa ctaaaaagtt cattttgaga
     9781 caccctccta tttataataa acaaataaat ccttaaacct attgagtgat tataattttt
     9841 agattgggtt atcttgcatt tttaaattta aggtaacttt ctactctaaa ctttaatctc
     9901 taatcaatat catcaactgt aacaacttac aattaaatct ttaataatca aactttagac
     9961 tatctatatt aaaactcaaa ttagatttag gatttatttg gatatcacat ttattttatt
    10021 aactactaat caattatttt tacttataaa cttattcttc cctaattcta ttattaaata
    10081 cttttaatta tttttcaata ccttaaatct ttattttatt aacctttatt cttcaatcat
    10141 aagttcaata aatttatctt cacttaaagg ttctgaaaaa taatgacctt gaatcaaatt
    10201 cacacctgaa gttacaacta aatccagagt ttcttttgtt tcaacacctt ctgctacaat
    10261 atccatgcca ttaatcttag caatttgaac tatagttttt aataattcaa attgtttttt
    10321 agaataggta atttgttcta ctaattcctt atctattttt aaattttcaa taggtaaatt
    10381 tgccagataa tttatactac taaatccaga accaaactca tctaaagcta tgctaatgcc
    10441 ttcttttttt agtttatcaa gagtaacatt tacaatttca aaagtttcca taagtactgt
    10501 ttctgtaact tcaacacaaa tatacttttt atctagacca caattctcaa tacatgataa
    10561 tatgtcattt gcacactgct tatgtacaat ctgctgaaca gataaattta attgcatttg
    10621 ttcaaaatct atatttttat cttttatttt tgtagttaca tcacaagcct tttttagtac
    10681 atatagacct acatccttaa taagtccatg cttttctgca taagtaataa cttcataggc
    10741 agaataataa ccgccctctt tatttctcaa tctaattagt gcctcagctt ttatataaca
    10801 gtttttacta acattaaaaa ttggctgata ccaaacttct agtgtttgtt cttccagacc
    10861 tttttttaaa tccttaataa tattattttc atggtaaatc tcttctgtca attccttatc
    10921 ataaacttta acattacttt tgacatcctt ttttgcatac tccaatgcaa tttgtacctt
    10981 ttccagaagg tcatatggat tttcaccatg atatggatat tgacaaatac caatattaca
    11041 ttttacatca aaaagtacat cttctacact taaagaacta ttaaatactt cattaacctt
    11101 atgtaaagta tctaagtaag attctttatc tattgaaatg cctaaaaata catctccatt
    11161 aatacgatat agataattag aaaaaattgt ttttaatttg ttactaactt cttttaaaat
    11221 aacatctcct atttcaatag aaaacagttc attaaatttt ccaaacttgc atatgtctat
    11281 taaaaataag tataacattc tctcagaatt ataatcaatc aatacttcga catcacgcat
    11341 ataccctcgt ctatttccaa tatcaatatt ataatcttta tacataatac gctgtacttt
    11401 tttataatat ttgtatatta ctttagacaa cttgtttatt tctaaatctg gatattcacc
    11461 aaatacaatc tgtttctttt catcaagcat atcttcacac tttttagtaa aatcatgaat
    11521 aggagacatt gctggaaatc ttctctcata atcttcaata gctgaaagta acagagattc
    11581 tgcatctttt ctatcatcaa cacacctaga tgagccataa ccaatcgtta aaaatacttg
    11641 tttattttct actttaattt ttcgtaaaag ctctaaggaa atcccttttt caatgttaag
    11701 caattcattt ttatcacaat tttcttctaa agctagaaca aaaccttcct cacttatacg
    11761 agcaataaaa tgagaacttt ttatgtaatt atctagattt tttactatat gtgtaagata
    11821 atcttgtctt ttttcaaagc tcataactgg agcaaattta ctataattag taatacatat
    11881 atacagcaat actatttcat tattttttct attcagatgc tcttgtaact taattaaaaa
    11941 accttctcta ttcaaaatac cagtttctaa atttttaaga ctctgttgat ttaattcctt
    12001 actaatcgac ttcttttcaa tgtaaataaa tgtcatcaca cctattaata tggaaataat
    12061 taacaatata acaaatacaa taaaatctat attcttatta ttccatccat ttttgtacac
    12121 aatgctaaga gtccaaacag tcggaagttt aaacttaatt tcatgtgatt tggaaaaatt
    12181 aaaatcttta gtagaaattt ctattgtaga ttttgaacca ttatctaggt gtacacgcca
    12241 aagttcatac tcaaatccag attcttttaa tttatttaag ttaaaagaat ctacaacttt
    12301 tttggcatct aaagctactg taatttctcc ccagtactct ccatttttat aaataggatt
    12361 agtaattaaa aacacatctc catttgaaaa ctggtcttta gaaggacctt ctacgactaa
    12421 tcctttgata gaatttgcta atgaataaga ataattcagt tcctgtattg gagtattgat
    12481 aatatctttt ttgacagaat tagaaaaaaa tgcagctaca ttatattttt ctacaactga
    12541 aatatatatg caagaattgt ttttaagaat ttcatctgca ataggtttta atttatctat
    12601 agaataattg tttgaaacta aaactgatgt caagtctttg ctataattaa atttttcagt
    12661 cagatttaaa ataatgttat tcttgtagac atttaacatg ttttctacta cttttgtttc
    12721 tttcttttct tgtttgtcaa aaacataaat agcaaattta ctaaaacata aaaatgttat
    12781 taatacaata atagtacata caaaaaattt tttagataca tttttcatat gatataaact
    12841 ccttccacat acattaaact aaatgataat taccttttcc attacgttta acatagtata
    12901 aagcttcatc agcctgtttt aataaattat ctatatcttt attgtctttt acactacata
    12961 ctacagctcc aacactacaa tgagtttgaa tgataccatc atctgttagt acttcagtat
    13021 gtaagcgaat agcaatctca tccaataaat cctccacctc ttctctcgat gtaaaaccag
    13081 ataacaacat aacaaattca tctccaccat agcgcccaat taaaccatta tattttatct
    13141 catattcacg aagtacatat ccaacatttt tcaaaaccat atccccaatt tcatgtccat
    13201 ataagtcgtt aatacatttc aaatcatcta aatcaacaaa tgaaattaca gataatttat
    13261 cattttcctc catttcaaca cgattataaa tttggtcaaa aaatacagta cgtgttacaa
    13321 caccagttaa tccatcagtc agacctttac ttgtgagctg aataatttct ttaaagtcta
    13381 cttcactagg tctttctgtt tgatatatac gtttttctaa ttcttgaaca gaacctaaaa
    13441 gcatatcaat aacttccatt cgttttttca tgaagctttt ctccatacaa atcaaaataa
    13501 taaatactaa agttataaca gatagaatag ctcccatagg aattaaattt aatgcaaaaa
    13561 tatctaagaa attaacttta caaaataaac tccaatttgt gttcttaatt ggtttatagg
    13621 acataaagta taattttcca ttttcaaaag cccaataatc acctgattta ttattaaaca
    13681 tatccttttc tacttttttt gcattggttc caagatattt tgttttctgc gataacttca
    13741 tatacttctc tccatatgca gtttcatcac ttgttaaatt cattatttga ttttcacttc
    13801 caaataatat gaaattctgt tgctgttgac cttttccttg tttgagtata tcctttatct
    13861 catcaaagta aattgctgca aatacacaac catctacatt tccattcttt attataggtg
    13921 atgcaatagt gtagtttaaa gtttttccat cagcccctgc tgcaaaacta tctgtaactt
    13981 gatatttttt tgttgcaaaa agtttctgca tataatcacg accagctaga ctaatattac
    14041 caccttcttc atcccataca attatattct tatcaacaaa acatatcatc atatacccaa
    14101 attcatcact atacattgaa agttctttac ttttatctat gccattcaca ctattatcta
    14161 ttatgtaatt atctttactc aaagagttca atagcttaat tgtttctcct accctatctt
    14221 caatacgatt tgatgttgta gataataaat cttgattatc ttttttcata acccatattg
    14281 tatttataca aaacatagaa agaataatta atataattac tatatagaaa gttataaaat
    14341 attttgctag agattgaaat gaaagttcac ttgacttatc tatatttctt tttttgtacc
    14401 tcataattcc tacccccact ata
//
LOCUS       DAELJO010000085        14372 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELJO010000085 DAELJO010000000
VERSION     DAELJO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.085"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      complement(231..314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(547..1389)
                     /locus_tag="KPB88_002176"
     CDS             complement(547..1389)
                     /locus_tag="KPB88_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0054450.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1641..3425)
                     /gene="pepF"
                     /locus_tag="KPB88_002177"
     CDS             complement(1641..3425)
                     /gene="pepF"
                     /locus_tag="KPB88_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0054451.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEMHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3491..4156)
                     /locus_tag="KPB88_002178"
     CDS             complement(3491..4156)
                     /locus_tag="KPB88_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0054452.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4307..4468)
                     /locus_tag="KPB88_002179"
     CDS             complement(4307..4468)
                     /locus_tag="KPB88_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG0054453.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4546..5055)
                     /locus_tag="KPB88_002180"
     CDS             complement(4546..5055)
                     /locus_tag="KPB88_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG0054454.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5261..5479
                     /locus_tag="KPB88_002181"
     CDS             5261..5479
                     /locus_tag="KPB88_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0054455.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5497..7443
                     /gene="feoB"
                     /locus_tag="KPB88_002182"
     CDS             5497..7443
                     /gene="feoB"
                     /locus_tag="KPB88_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0054456.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7622..8677)
                     /locus_tag="KPB88_002183"
     CDS             complement(7622..8677)
                     /locus_tag="KPB88_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0054457.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8725..9525)
                     /locus_tag="KPB88_002184"
     CDS             complement(8725..9525)
                     /locus_tag="KPB88_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0054458.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9518..10309)
                     /locus_tag="KPB88_002185"
     CDS             complement(9518..10309)
                     /locus_tag="KPB88_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054459.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10349..10771)
                     /locus_tag="KPB88_002186"
     CDS             complement(10349..10771)
                     /locus_tag="KPB88_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG0054460.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10787..11266)
                     /locus_tag="KPB88_002187"
     CDS             complement(10787..11266)
                     /locus_tag="KPB88_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054461.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11398..14154)
                     /locus_tag="KPB88_002188"
     CDS             complement(11398..14154)
                     /locus_tag="KPB88_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054462.1"
                     /translation="MRDEILSFINECVLFNNFDELKKESGIVTEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4666 a         2481 c         1622 g         5603 t
ORIGIN      
        1 atattgttac aaaaggttta tattctgcat ctcaatgttt ataaaaactt tattttctta
       61 ttataatact attattcttt taaaaaaatt tcccttttcc aacaaatggc tactttaaaa
      121 gccaaaattt tacctatcaa taaaaatctt aacgactaat actcttattt tcaaattttg
      181 caacatattt gttaatttta aaacaaaaaa agactatgca atataaaaat tatattcata
      241 gccggttgca ccactaactc catatatctc tagatgccca ataataagat atacttctca
      301 gtttctaaaa tatttttatt aaaaatcttt tatatatttt cataaatttc ttttttattc
      361 taacattgta atattatttt gtcaatattt ccatattctt agagataaga tactaaaata
      421 cctattttat ctaaagatat ttatatttct acattcaata atagacaata ccctgcaagt
      481 taaagcatca tttttatgag cttatctcac agggtattat atgtttactt tatattataa
      541 ctcttgttat tgattaactt taataaatcc tttttctgaa acaagtgctt gtccttcatc
      601 acttaatata taatctatta aagctttacc tttttctgac aaaccatctt ctttattaac
      661 taatataaat ggtcttgata ttggatattt attagctatt acatttgcat cagttgcttc
      721 aacatcattt atttttaaac tatttacttt ctcatcaaca tatccaaatg atatatatcc
      781 tatagcattt tcatttcctt caactgtagt ttttatattt cctgaaccat cacttatttg
      841 tgcatcttta gttagttctt ctgaatcaaa ccctacaatt tcttggaatc catctctagt
      901 tcctgaacct tcttctcttg atactattac tattggtgca tcatttcctc ctacttcttt
      961 ccagttagta atctttcctg tatatatacc tttaacttgg tccattgtca gattttttac
     1021 tgagtttgca gtattagtta tcaatgctat accatctatt gctattttag tttcctttaa
     1081 tccagcactt ttttcttcat cttttaaatc tcttgaactc attcctattt cagatgttcc
     1141 ttctatagca ttttttatac ctccagaaga accaatctgt tgtacttcta cactaacacc
     1201 ttgatttttt tcttgaaact tttctccgat agtttccata agaggaccta ttgaagttga
     1261 acctgaaaca gaaactttgc ttgaatctcc tgaatcacct gaacctcctg agttagagcc
     1321 acatcctact gctaaagctc caactaacat tgtactagct aaaagaacgc ctattttctt
     1381 tttaaacatt ttacttaccc ccgcttatat tttaattttt tgttgtcatt tttctatatt
     1441 ttaataatac agattctttg ttaaatgaat attagagcag ggttaagtaa atgtaaatat
     1501 tgttaaatac atgtaaaaac ttaattttat atgatttatt cttaattaga ttaacttttt
     1561 aaagttactt taggcagata taaacataat acaactcata ttatcagaag tcttcattga
     1621 aactatcaac ctatcacatc ctataattct ttttctagtt tattaactaa ttcagcaaat
     1681 acatttaaag cttcctctat agattctttt ttcgtcatat ctacaccagc agattttaat
     1741 tgttctagtg ggtattctga ccctccactt tttaagaagt ttatatatct atctactgca
     1801 gtacttcctt ctgaaagtat ctgttgactt aaagcacttg cagcagaaaa ccctgtggca
     1861 tacttatata catagaagtt tgaataaaaa tgaggtattc tcgcccattc tattcctatt
     1921 tgtttatcta ctattgcaga ttttccgtaa tacttttcat ttaatttgta gtatatatta
     1981 gtaaattcat cagcagtaag tggttctcca ctctctaccc tttgatgtac aattttttca
     2041 aattcagcaa acatagtttg tctatgaact gtagttctaa attgttctaa ataataattt
     2101 aataagtata tagtttcatc cttagactta gaattttcta ataaataatt tataagaagt
     2161 aattcattta atgtagatgc aacttctgca acgaatatct tatagtccga atataaaaat
     2221 ggttgacttg ttcttgaata atgactgtga acagagtgcc ctaattcatg aattaaagtg
     2281 aatagagaat taaggtcatt tttatagctc attaatatat aaggatgtga atcataactt
     2341 ccccaagaat aagctcctcc ttttttacct tcattatcat atacatcaat ccacccttca
     2401 tcaaaagcac tttttatgac cttcaagtat tcttctccta aaggtttaag tgcctctaat
     2461 atgatatcct gtgctttatc atatggtata tccatgtcaa atttatctgt caaaggaaca
     2521 tacaaatcat acatgtgcat ttcattcaat cctaaaaatt tctttttaag ctcaacatat
     2581 ttatttagat taggaatact tttatctata gctgatataa gattattgta aacatctaaa
     2641 cttacatcat ctgaaaataa agacgcttct attgctgaat tatactttct agtttttgcg
     2701 taaaatatct ctgattttat tccaccataa agcgttgagg caaatgtatt tttatatttc
     2761 tcatatgttg aaaattcacc ttcaaaagcg tcttttctaa ctctactatt cttacttttt
     2821 ataaataaag agtagtttga atgagaaagc ttaaccttat tcccctcctc atcttctatc
     2881 tcaggaaatt ccatatctgc atatgctaac atatcgtata cattttctgg aattgtcgat
     2941 aattctgatg tagctgcaag tatttcttcc tctcgttcac tcaaagtatg tggtttatct
     3001 ctcaatatat cctttatata ttttgtataa aaggatagct tctcatcttt taagtattca
     3061 cttaacttat tctcttctat cgccagtatt tctggaacta tataagaagt agccacacct
     3121 aaatcagtag ataacatttc tgtcttagtg gctcttcctt gattatcatt aattctagta
     3181 tcttcatgtt gtttcatatg agtatataca tataaatttt gaacaatcct agatgcattt
     3241 tcagagatat ttaatacttt atataagttc tccttactct cagaaagctt ccctttatat
     3301 tcttttactt cttggataag attttttact ttacttatgt ctttttcaat ttcctcttta
     3361 ctggagtaca tcttatctat cttccatttg tacttttctt ctattgtact tctatcagtt
     3421 cccatatcat acacctctat tttaatttgt tttatatttt attcacttta caaaatatat
     3481 tgtatatatt ttagtccaaa taatctacat ttattactcc ttctatcata tttatttctt
     3541 tcataacatc gtgtaagtgt attctcttag cataagatac aaaaaaatta accttccagt
     3601 attcttcatt acgacattct atttccatat ttcttacgat aagttgattt ttttcaaatt
     3661 tatcatataa aaaaggtata gagtccaaat tgcttaattt taatatcaat acattctttt
     3721 cttggctaaa taaaaacgta ttgattatcc taagcacaaa taatgctatc attacaaata
     3781 atccacctag aattgccaat tcataataac catatccaac tgctatacca atacatgcag
     3841 ttgcccacat cccagcagct gtagtaagcc cttttactcc acctttattt tttaaaattg
     3901 ctcctgcacc taaaaaacca attccagata atacttgagc aggcattctt gctgggtcag
     3961 catttgtata caaattataa tcaaagaata attgaatcga agttattgaa acaatacaag
     4021 aacctattgc aattagtata tgtgtcctaa atcctgcaaa ctgtcttatt ttttctctct
     4081 ctaagcctat tattcctcct attattagtg ctaatagtaa ccttattgct atctccttac
     4141 ttcctatatc aatcataata taaaaccctc ctgacttaat tacaacttat gtcctacaga
     4201 taatttacac atcagaaata ctattttata tcttaatatt attattaata tatgtaaaat
     4261 atagccatct gttaattttt gatacaaaaa aacccacaac tgtgggctat ttgcttaggt
     4321 acatgtattt atttattaat atatctattt tcttacttga ttctactaac tcttcttgaa
     4381 atatttctgg atattctatg tatgtattta tctcttccct aactgcttca atctcatttt
     4441 ttaacgcttc taattcattg ttcgccataa taatcaccac tattacaaat ttgtatattt
     4501 ttatatacat tttatcaaac aatattatat tttgtcaata ttatgtcaat aaaacttact
     4561 ataaaaatgc tttaatttag gtattgtata cctggatact aagcctacaa acataccagt
     4621 tactaatgat gccattaaca taaatggtaa gtaccctata gcaagtatat tttctatgat
     4681 taaacttgca actattaatt gacctatatt atgccctatt gcacctataa tgcttatccc
     4741 tattagtgat aaatctttta ttttactcgc taaatacata aataaaagac tcagataagc
     4801 tcctcctata ctaaatagta gatatgataa agggcctgca aatattgatg acaaaattac
     4861 cctgatagta acaattataa cagtatcttt gacacctaac aacattaatg aaattaaagt
     4921 tataatattg ctcaaaccca attttgcccc tggaaatata actataagag gatttggtat
     4981 atatgtctct aatacatata aagctaaact ataccctatc ataagaccta aaaatgtcat
     5041 ttttctagtt ttcattaagt taactcctcc aaattttatc tatcatctta cattataata
     5101 caaaatatat aaaaacacct aatttagact aaaatatttt taatctattt agaataaata
     5161 ttagttattt caatgtgcaa ttacctctat aaatcttgac tttttgtatc atacttgtta
     5221 caattttaat gataatgaat ttcattagga ggtgttaata atgactgttt acgatttaaa
     5281 aataggagaa aaaggattta ttaatagtat agatggagat attaaattat ctaaaagatt
     5341 gcttgcactt ggatgtataa atagtactga aatagaagtt aaaaaggttg ccccacttgg
     5401 agacccaatt ataattagat ttagaggctt tgaccttgcg attagaaaat ctgatgctaa
     5461 aaatatttct ttacaataat agaggaggag ctacccgtga ttaatgtagc attattagga
     5521 aacccaaatg ttggtaaaac tactgttttt aatctactta caggttcaaa tcaatatgta
     5581 ggtaactggc ctggtgttac tatagaaaaa aaagaaggat ttcttggaaa agagataaag
     5641 gttgtcgatt tacctggtat atatgcaatg gatactttct caaatgaaga aaaggtttct
     5701 aaatcatatt tagaaaatga agatgttgat gtaatagtaa atgtagttga tgcttctaat
     5761 ttatcaagaa acctttactt aacaactcaa cttatgcaat ttaacaagcc tatagttata
     5821 ttgcttaata tgttagacat tgcagaatct aaaggagtta atatagatgc caaaaaatta
     5881 agtgaagaac ttggagtaat tgttgttcca attatagcaa aaaagaaaaa tggtgttgat
     5941 aggatagaag aagagattaa gggagctacg caaaaagctt ttatatatca gaaaaacttt
     6001 ggaagtgaaa cagaaacata taaaaaatta gaatccattt tatcaagatg tacaaaaact
     6061 acatatacac aaaagaaatc tatcagtgat aagatagaca atatagtctt gaatcctata
     6121 ttagcatatc caatatttat tggtgtattg ttcctcttat ttaaatttac ctttgactgg
     6181 gtcggaggtc cactacaaga gggatttgct agtcttatag aggcttatat ttctgcacct
     6241 gttagtgata tgttatctaa ctctagccca tggtttaaat cacttatagt tgatggaata
     6301 attggaggtg ttggtggtac tttaccattc ttccctttaa tatttacatt attctttggt
     6361 atatcgttgt ttgaagatag tggatacatg tcaagaacag catttttaat ggataaagtt
     6421 atgagaaagg ttggtctatc tggtaaagct tttataccta tagttatggg tatgggatgt
     6481 tcttctccag ctataatggc aactagaaca ttggaaagcg aaaaagatag aagagttaca
     6541 gctcttattg caccacttat gacttgtgga gcaaagctac ctatatatgc actatttgta
     6601 gctatattct ttccacataa tgctgcttta gtaactacct cactgtattt ggtgggtata
     6661 gttatggcaa ttgtagttgc attattctta aataaaacag cctttaaaac agaggctgaa
     6721 ccatttatat tagaattacc agaatataga atccctacta tagatgcact tattaaaaat
     6781 acttggaata aatcaaaagg tttcttgata agagttgtta ctgtcatgtt tgcaatgtca
     6841 gtcgtaattt ggggactttc ttcattcaat gtttttggat atacagaaga tataaatgta
     6901 agcttcttgg ctactctagg tcatattata tctcctatat tcaaacctct tggttttgat
     6961 gattggagaa cttcagttgc tattttaagt ggtcttggag caaaagaaat cgtagtaaac
     7021 tctttaaata tactatatgg taatctaact gttgtcttac caacaatatt taatggagtt
     7081 actgcatata cattcttgat atttactgct ttatatactc catgtatagc tgcacttgca
     7141 actatgaaaa aagaatatgg caataaaatg atgtttacat catttgcata tcaatttata
     7201 ctagcatgga taatggcttt tatagttaaa agtataggtg gagtactatt tatgggtaat
     7261 tcaataatgg aagctttgat aggtggaata attatagtcc ttgcactctt tattctgttt
     7321 aataaattca agtctacaaa aaacggaaat gtctgtagtg gatgttctat ttgttctgga
     7381 tgtccaagtg ctaattcttg ctcaagtaag gaagacagca ataaaaaaaa taatgcacac
     7441 taatatataa atattttagt ttaaaataag ataaaagtgg gtgtctcaaa attaactttt
     7501 tagttcatga ggcactcttt tttatataaa atcaaatata ttttcatcat ttcaacagta
     7561 aaaaagagct tatctagtaa aaaagaggct tatctctatt ttgagacaac ctcttttttt
     7621 gttacttatt cagtttcttg ctacctaact tcatgtggaa cctagtatct tttggaacac
     7681 ttgggtctac acctcttaat gtaggtattt ccccacatat tagttgtata ggtactataa
     7741 aattaaatgt ttgatagtaa ggattatctg tgatatcaat ctttaaattt ttatcatttt
     7801 ctgtaacatc tctacctata gcaaatacat tttccgtcca accagaaagt acatcaatca
     7861 tcttagttac tctaggttct tttcctgtat ctagtataaa tatagtagaa tcactattta
     7921 tagcattata tataccatgt ataaactctt caaactcata cccagttaca ggtattctca
     7981 ttgtttccaa taattttaat gctgcttcta atgtatctcc atatatatca gagtgaccta
     8041 ttattcttat ttctttagaa ttaactaact tttctttgtt tctttcaatc cattgtttag
     8101 agagcttata cactgcttca aatctattaa ttgcatctaa tatcttattt atttcctcat
     8161 tgtacttttc actagatatt ataccttttt ctctagctat ttgaagacct aataacatta
     8221 agtttaactt tgtacaataa tatccttttg ttttagctcc agatttttct tcaccacaat
     8281 ttactgttaa tatataatca gaaatctcat ctattaaagc attcttacaa cctgccatag
     8341 atgctatttt acagccctta tcttcagcta acttcatagc gttgtatgta gaataactac
     8401 tcccaccttg agatacacct actactaggg tattttcatt atcaaacttg aatgtatctt
     8461 cagttatcat aaatggatac attttcacaa catctatatc taataaattt tgtaaatatg
     8521 gttgcacttg cactcctgag tgatagctag tacctgaacc tgttattata atctttttta
     8581 gatttgttct cttaacttca ttaaataagc tatctgcatt agatataatt tctctcattc
     8641 taactggtgt ttctaacata taatcttgta ttgtcatttt atacaccttc cttatctata
     8701 tattcaataa attataatta atttctataa caaacctata gcatgtaaag cgattcctgt
     8761 tactatagtg cctatagtta attttgttac acttaccttt ctctttaaca tgtagtatat
     8821 tacaaatgca aaggttaaag gtaacatatt aggtattatt ttatctagta tatcttgtaa
     8881 aattacttct gcaccattta agttgaattt cagaggagta gttatactca acatagttgc
     8941 aaccattcct cctacaacga ataaaccaac agttgtagcc atagacatta ttttctccat
     9001 aagtccctct ctttgtattc tttcaagaga atttacccct acttgataac catacttaag
     9061 tccaaatatt ctaagggcaa atgctggtat attgtataat agtaagaata atagtggtcc
     9121 aaagatatta ccttcttttg caagtgatac tcctactcct gctgctataa tcctaaatgt
     9181 tccccagaat aatgaatcac caattcctgc aagaggtccc ataagagctg ttttaacagc
     9241 actaattgaa ctttcatcaa attctggatt attagcattc tcttcttcca tagctgctgt
     9301 tatacctaat atagcaggaa ctactactgt cgttgtatta aaaatctcca agtgcctttc
     9361 tattgctttt gcttgatttt ctttcttatt gtatagcttc tttattgcag gtaacattga
     9421 ataacagtaa cctaagtttt gcattctttc gtaattaaat gcaccttgaa gagcaaatga
     9481 acgccaaaac atacttctta attctttttt tgttaattta ttcaaagtca atctctcctt
     9541 ccgttaaagt attgattgct aattgaggat tttgattatt ttctttgtta ttgatataaa
     9601 agtttagtac aaatgctaca catactccaa ataaagctat agcagttata tctaatccac
     9661 cataagatgc taacaaaaac cctataaaga aaaatacaga catctttttt gaaagtaaca
     9721 tatccataag caatgcaaaa cctactgctg gcaacaatgt tcctgcaaca cccagtccgt
     9781 ctaatataac tttaggtatt gcatttatta atgctgtaac tttatcagca ccaatcataa
     9841 ttgctaaaaa ggttggaact gatgtactta aaaagaataa tattactggc aaccacatca
     9901 ttatggtaac tttcttaaaa tcagcctgtt tagcatagaa aactgccttt tgagaaaagt
     9961 acgaatttat tattctaact aatactccaa gagattgtgc taagactgct actggtactg
    10021 ctactgctaa agcaacttct gcaccattac ctgataatat agcaaaagct gtacctaata
    10081 cacctcccgt tactacatct ggaggagtag ctgcacctat tcctgcaatt cccatccaaa
    10141 ttaattcaag ctgtgcacca atcataattc ctgattttat gtctcctaaa actaatccta
    10201 ctaacatacc tgtaactatt ggcctatcaa gcatagattg accaaatata cgcccatcta
    10261 gtataccaac tcctgctata atagctaaca tagtagcttg tactaacata ttgaacctcc
    10321 attttaactt agtttaattt acattaaatt acattacatt tttgattttg ttttgatttt
    10381 ctaataactc atttacataa acaattccat tttttgcttc ctctaatcct gatttaacta
    10441 ttaattttgt atcctcctca actaataaaa gtatagataa aagcattggt aaatttactc
    10501 ctgtcaataa ttcaaatttg aacccttgta aaagtaagtt tgtacatatg tttgccacac
    10561 ttccaccaaa aacatctgtt aagacaacaa attcttcact atctttatta tttgataata
    10621 tacttctaat ttctttctca gcatcatctg ctgatttatc cgttgtcata caaaaatact
    10681 caatattagt taatgaacca attattaatt tacttgtgtc aacaagctct ctggacaatt
    10741 ctccatgtgt tgcaattaga aattttttca ttctctttct cctaaattat tttattaaat
    10801 ctgatagttg tttctttttg tctctaggta acattctaaa ttctatatca atccctaaat
    10861 cacctatttg tttaaagtta tttatatcat catcatcaac agcaactaaa tctgtaagtt
    10921 tcttcttccc ttcctcatat cttaatcctc ctacatttat ttcttttaat actcctcctg
    10981 aatttttagc tacaactaaa gcatctttgg tattttttac taacactaat acattgcttt
    11041 tctttgaaga agcaaatttt tctactattt ctccagattc ctctaccccc cttatatata
    11101 aagttacatt gctaggtttc gctaagttca gtgacatagc tttcatttgg tcattttttg
    11161 cttcatcatt tgcaactagt attacattta tacctagtga tgttgcccat ccatatgcaa
    11221 cctgaccatg tatcaatctg tcatcaattc tcatcaaaga taccattttc cccaaatcct
    11281 ccatcataca ttaaaatttt tattgtttat actctcattt atctataaaa gcaaaaagta
    11341 tgccaaatac tggcatactt ttttaaattt cattcactta ttttgtaatc ttgtatttta
    11401 ctgtggtaga ccatagataa tattataaat atatcctatc tctgaaattg gtatttgcac
    11461 actataactc ttttctagca cactaaatgc attttttatt agttttactt cactatgatg
    11521 gtttttgatt aacaaatcca tatcagtata ctcttcaatt tcagtttgtc taactaatct
    11581 ttcaatcata caacttaagt gaacatataa agaaatcttt ttatcatttg aaagtttttt
    11641 cttttgaata ttttctatag cattaattcc ttcttctatt ctctctatta tcttttttga
    11701 atctaaaata gttagactat ctattactct aattaatgaa aaattcttta caatagaatc
    11761 atttatgttc tttatttttt cattttctac tactctgcta aatattctaa aaacatcatc
    11821 ttctcctttt cctgatatta tatcctctaa taatataaaa tctaccccat tcacatttgg
    11881 attggcagtt cctactatag ccattacatc atacatttca aaaactggtt ctttatctcc
    11941 catagaaaca agactatcat aatcataagg tattattttg acatcaacta ttccaattaa
    12001 actttcggaa agtagatgtt ggatttttat agcagtacct atccctgtaa aacaagatga
    12061 tattatagca tattctttat ttttttgagg ataatacaat tgtatattag gtttagtttc
    12121 ttcatccata atttctgcaa tctcttcaat gtttttacct tgcattaata aatttcctac
    12181 ttctagtgca gaaatagtgg atatttggtc tataaataac attgggctat ttatcttatt
    12241 ctttaaatct tcatatataa gattaagaga gcccatatca aataacacaa ttaaaccatt
    12301 ctttatccta tctccctcta gatatctaga catttgtttt gatacatcag caatagtagc
    12361 atctataggc atatcaatag ctgtaaatat atttttaccc aacatccgat tacatacatt
    12421 cgccatacta ctggcagtag catatccatg tgctataata gtcgcattaa cttgattaaa
    12481 tttcattctt ataattgttg atttaataaa tataatcatt atgattgtat ctacattatt
    12541 aagatacata tccaatctat tcgttattaa tgttgatatt ttacttatta tagagtattc
    12601 tttgtaactc tcttttttta aatatattag taatttattt tctaaagctt tatactcatc
    12661 agacaattga taagattcgt ccttcaaata aagataagac gaaaatgcca atacattatt
    12721 cccatcatat tttatattgt tattaacttc taaatttcta aatatatcct gcattatcga
    12781 tattataagc tccataagta cgttattatt ttcatcatta tttgaaaaga ctaaattgtc
    12841 cattagtcta atcattagat tagaactctt tttactaaat ttttcctttg ttatttcatt
    12901 tttttctagt ctatcaaagt aatctaaaca ttttatgaaa ctttgataga ttatatttct
    12961 tttatttctt ccatttgaca tttcaatact aaattgttta ggagaaaaga taatgctttt
    13021 gcttttacca ctttggaatt gaaatatatc tacattttct atataaagat ttctaggaag
    13081 gtcttgaaga gtaacaaaaa tatttccatt tcttctttga ttttttgcat aagctcgtcc
    13141 acatgcatat tttattacat ttttacattc acctatattc cccttgaatt ttatctttaa
    13201 aatcgcattt aatgtaattt gagttatctc tataggcaaa ccaagtacct tactttcttc
    13261 ataaataaaa tgttctacaa tctttctttt ttcaatagtc cctctttctt ctatattggg
    13321 aacataaata tttatgggta ttcttcttaa aaaggtttgt aaaaattcag atagactttc
    13381 tgttgtagca aatattaatc ttaccctagc ttttctaatt actccattct ctccaatcct
    13441 agaaaacgta cctgtatcca taaatgtaaa taacttttcc tgaccttcac tatttaatct
    13501 atgtacttca tctaaaaata aaacaccacc atctgattgt tctattagac ctgctctaga
    13561 ttcatttgct ccagtatatg ctccttttac atgcccaaat aatagactag acaataactc
    13621 caaattatta taatactgcg cacagtttaa cacattgtat ggagaatttt tttctaatac
    13681 attactgctt acactatatt tgtatattat cttggctaaa aaagtttttc ctactccact
    13741 ttcacctgtc agcattattg taagtccgcc atttggataa agtacagcgg tttttatttg
    13801 ttcgattact tcttttaagc tattatcata tcctatgata ctattaaaca cagtctcttg
    13861 ctttaataat ttacactcat ttttaagttc atctaaattt tcataaatac ttttttttgt
    13921 aggtcctact acatcattta ttttattttt aggtaaaaaa ataacaggtc tactgtttat
    13981 ctttataaga ttatcagatt ttactaaatc atttaataat ctacttgatt gagttctact
    14041 taaattaaac ttttttacta tatcttctgt tactatgcca ctttcttttt taagttcatc
    14101 aaagttgtta aataatacac attcatttat aaaacttaaa atttcatccc tcatgttcaa
    14161 cctccaatac aaatatcaat aatactataa tattagtata tcatttatat atttatgaaa
    14221 aagataatta tattcctatc ttgagtctct tttgtaaggt tggaaaatca aactttttat
    14281 aagtataaaa tctagttttt ttaacataat ctcaagattc ttattttccc atatatttga
    14341 tatagaattt atataagaat atatttattg ta
//
LOCUS       DAELJO010000086        14301 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELJO010000086 DAELJO010000000
VERSION     DAELJO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.086"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            338..1129
                     /locus_tag="KPB88_002189"
     CDS             338..1129
                     /locus_tag="KPB88_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG0054463.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1158..2378
                     /locus_tag="KPB88_002190"
     CDS             1158..2378
                     /locus_tag="KPB88_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG0054464.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2398..3093
                     /gene="pxpB"
                     /locus_tag="KPB88_002191"
     CDS             2398..3093
                     /gene="pxpB"
                     /locus_tag="KPB88_002191"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG0054465.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3123..4109
                     /locus_tag="KPB88_002192"
     CDS             3123..4109
                     /locus_tag="KPB88_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG0054466.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFGGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4328..4894)
                     /locus_tag="KPB88_002193"
     CDS             complement(4328..4894)
                     /locus_tag="KPB88_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054467.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5246..6313
                     /locus_tag="KPB88_002194"
     CDS             5246..6313
                     /locus_tag="KPB88_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG0054468.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6332..7492
                     /locus_tag="KPB88_002195"
     CDS             6332..7492
                     /locus_tag="KPB88_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0054469.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7505..9601
                     /locus_tag="KPB88_002196"
     CDS             7505..9601
                     /locus_tag="KPB88_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG0054470.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKVENLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPFEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPSLVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9760..10104)
                     /locus_tag="KPB88_002197"
     CDS             complement(9760..10104)
                     /locus_tag="KPB88_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0054471.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10396..11286
                     /locus_tag="KPB88_002198"
     CDS             10396..11286
                     /locus_tag="KPB88_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0054472.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11504..12790
                     /locus_tag="KPB88_002199"
     CDS             11504..12790
                     /locus_tag="KPB88_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0054473.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTVTFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12879..13322)
                     /locus_tag="KPB88_002200"
     CDS             complement(12879..13322)
                     /locus_tag="KPB88_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG0054474.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13346..14140)
                     /locus_tag="KPB88_002201"
     CDS             complement(13346..14140)
                     /locus_tag="KPB88_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG0054475.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         5488 a         1636 c         2503 g         4674 t
ORIGIN      
        1 aagaaaaatg attctgtctt gcatataaag tgattaaaaa tgaaaaatac tttaaaaatg
       61 aaggttaagg cccttatttt aagtgaaaat aattaattat taactaaaaa atggtaaaaa
      121 gattttttga aaaagaaata caaatgaatg ttcaattaaa ataattgcaa aaatattcct
      181 taaatggtaa aattatatta attaaatgca atagaatagt tgcaaaaata aaattgaata
      241 caagtgataa aatatttcat gcaatatata aaataaacaa aatttaaaaa ttaaaactac
      301 ttattctatc aaaatgttag cgaggagggg tcataaattg tataaagtag atttaaatag
      361 tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag ttttaaaata
      421 tatttcatca gccaatatag cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa
      481 aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag gtttcctaga
      541 tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta aagattatac
      601 aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta atatacaaca
      661 tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt tagctatggg
      721 gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc tatataatag
      781 tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag tatttgcaga
      841 tagagcttat gataataatg gttttttggt tccaagaaat gtagaaggag cagttataca
      901 tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa ctgtagaaac
      961 tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg gggacaatcc
     1021 aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaag gtatagaagt
     1081 ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag actgataaca
     1141 tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa aataaaaaag
     1201 atgtcggagc acttataggg gctgcattta taatggcaac atcagcaatt ggaccagggt
     1261 ttttaactca gactgcacaa tttacccaag attttggacc aaatttttca tttgtaattt
     1321 taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta attggtgtat
     1381 caggacttag aggtcaggat atagcaaata aaataatacc tggattaggt tatttagtag
     1441 cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt ggagctgcac
     1501 ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta agtggattaa
     1561 tagctatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag ataactaaat
     1621 ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat catcctcctg
     1681 ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggactg atattcccaa
     1741 taataactct acttggagga agtgttggag gttatataac ttttgctgga ggacatagat
     1801 taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa tcttctcttt
     1861 tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata ctggcagtgg
     1921 tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt aaatttagtg
     1981 caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca attacatcag
     2041 ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct tttatagata
     2101 aatatgaaaa atattttata atagcattta tagcaatatc aacacttata atggcattta
     2161 taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg atacttccaa
     2221 tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga gattacaagc
     2281 atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca tatacaggaa
     2341 taacttctct tagcagccta ggtgctttat ttgcttaatt tagaagaggt gacatttatg
     2401 gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg aaatgagata
     2461 tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat atcgaatctt
     2521 aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag ttataatcca
     2581 ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga taatttggaa
     2641 agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga tgaggttttt
     2701 ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga agttataaaa
     2761 atacacacaa gcagagagta cttgatatat atgctaggtt ttactcctgg atttccatat
     2821 ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag gactaaaatt
     2881 tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat tgatagtcct
     2941 ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa tagagaagaa
     3001 cagattttgc ttagagctgg agattttata aaatttgttc caataacatt agatgaattt
     3061 atagaaatcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag ggagtggtat
     3121 atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac agaggtagag
     3181 ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat gcacatagag
     3241 taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt ttaatgttag
     3301 gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga gatttagggg
     3361 ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat ccaggtgatg
     3421 tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata gctggtggaa
     3481 tagatgttcc acttgcaatg ggaagtaagt caacttatac aagagctaaa attggtggat
     3541 ttggaggtag agcacttaaa aaaggagatt atataaatac ttttattcag gaaaaagatt
     3601 ttacaataaa taaaaaatta agttcaaaat atattccaac ttatagttca gaaatcgtac
     3661 taagaattgt aaaaggtcct caatttgatg ctttttctaa tggtgaagta gagaaatttt
     3721 tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga ctagatggag
     3781 agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct tatggagcta
     3841 tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag actagtggtg
     3901 gctatactaa gatagggaac gttataagtg tagatttgta taagttagca caagcaaagc
     3961 caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga ctattgagag
     4021 agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga gttgtaaagg
     4081 caaaagtatt aaatgatata gcagtttaat ttaattttaa caaatacaat ttttattata
     4141 atttataata taacttacaa atagctattt actatttacc ataattaaga tggtttagat
     4201 tagtcaaata gctatttttt tagtttgagt aaatatttta aatttaaata aatacttcaa
     4261 ttttaaaata aaagcaatat ttaaatctat tatattatct acaaaaagta ggtatgttat
     4321 attgagttta tttagatttt attggccaaa ttggaggtga tataactcca tatccacgag
     4381 ctattgtatc tttacttgga ttgatgattt tatgtggtac attagctttt ataaatgttg
     4441 agtcacctgg atttaatcta actacctttt cacccataca aatatcaacc acaccactcg
     4501 ttaccaccat aatttcttct gatgcatgat atatataatc tttggcatcc tcactttctg
     4561 gtttaagttc aaactctaca aataaaattg aaggtgtaaa ctcagttgta ggcattggag
     4621 atactatctc gtaaaacatt tcactctttg gaaatttcat cataactcta tcttcttttc
     4681 taatgactaa atcatcagta ttattgtcat ccataaacat atataaagga gtgtctaaaa
     4741 cttcagcgat ttttctcaat gaagacaagg atggttcaat agcatctctt tctatttgag
     4801 agatataact tatagataaa tctgtcaatt tggaaacatc acttaatgtt tttttcttgc
     4861 tttttcttat atttctcatc ttttcaccta gcataagaac ctccaaataa taaaattaat
     4921 ataaaatatt cgcttaaaac aaagtaatta tagcacaaca tagaagtagt ggcaataaga
     4981 atacatagta gattttgaca attaaaattt aattaaatcc aaataaaagt ctatgaaaat
     5041 gaattattgt taaattaata taaagaaaat aataataaaa aaatttaata gaagagtatt
     5101 aattgaatta agattataac attatatttt ggaaaagagt aaattttcag taaattatct
     5161 tgacatatat attgaagtaa taatataatt taaagtataa aattttaaaa attgaattat
     5221 aacttcaaaa aataaggagg gtaatatgaa gataacagat atcaaatttg aaaagcttag
     5281 aattaaatta aaaaaacctg tagtagtttc atttggagta attgagtatg gagaaagtgt
     5341 aattttaaaa attgaaactg atgaagggta ttgtggattt ggcgaagcag cacctcttgc
     5401 agcagttaca ggtgaagttt tggataatgt tctttcagta ttgcttatgt ttaaaaaaga
     5461 gttaataggc aaagacccac tagatattga aaccatacat accatcatgg atggaattat
     5521 tataggtaat acttctgcaa aagcagctat tgacatagct ttatatgata ttaaaggaaa
     5581 aattatgaat gcacctcttt ataaagttct tggaggattt gatagtaaag tccaaacgga
     5641 tattactata agtatagata agccagaaaa aatggcagta gaagctttag aaagagtaaa
     5701 agaaggtttt aggatattga agcttaaagc aggtataaat cctgaagacg atattgaagc
     5761 tgtaaagtta attagagaag ccgttggaga gagtataaga attagaattg atgcaaatca
     5821 aggatggaac gtaaatagca gtataaatac tatcaaaaag ttagaagaat ttaatgttga
     5881 tgcaattgaa caagcacttc cacattggga tttagatggt actgcttaca taagaaataa
     5941 aagtaacaca aaaattatga tagatgaatc attacattca ccaatagatg caattaaggc
     6001 tataaaaaag aatgcagttg atacatttaa tattaagctg atgaaatcaa gtggaatata
     6061 tcctgctata aaaatcaaca acatagctga ggcaagtgga gtaaattgta tgttaggatg
     6121 tatgctggaa acgagaattg gtataacagc agcagcaaat ttaatagcat ctaaaaagaa
     6181 cataacagaa gctgaccttg atagttttat gttttgtgaa gaattagaag gtatatctgg
     6241 aggatttgta atggatggag atattatgaa tcttgtcaat aaaccaggtt taggaataga
     6301 agtaaactta tgataaattt ggaggttaaa tatgaataca cttttttcaa atccaggtag
     6361 tatacttgct gtaatgacat caatgattgc attgggtttt tacttacaaa gatacaaagc
     6421 cattaaaagc ttaggaccag cacttacaat aatcataatg ggaataatac tttcaaactt
     6481 aaaagtagtt ccagtaagta ctgagttata tggaactatt tcaacatatg ctattccagt
     6541 ttctatgact ataatgttga tgagtgttga cttaaaagaa atgacaaaat tatcaagaga
     6601 acctttaatt gcaatttttg tagctgtatt aactgtaagt atcatggcat ttttatttgg
     6661 attagtattt gcagaaaaaa tatcagaagg ttggaaggtt gctggaatgt ttgtaggtac
     6721 atacactggt ggaagtgcta atcttacagc tattggaaca ggtcttaatg taagtagaca
     6781 aactcttgca gcagcaaatg cagcagacta tgtaattgga gtaccaacat tgatatttat
     6841 gtttgctcta ccagcaatat taaaaaattc aaagaagttt aaaaaacttt ggccatatca
     6901 tgtagaagaa tcagaattag aggattgtca aaatgaagaa tttatggagt caaaagaatg
     6961 gagtattaaa gatatagctt ggatgttggc tataggtttt gttgttacag aggtttcaac
     7021 aatacttgct ggatatttca attcaagttt tagtagtgca gcaagaatac ttttagttac
     7081 aacaatttca attataattg ctcaattaaa gccagtaaag aaactaaagg gaaatttaga
     7141 tttaggatta tttgttgcat tattcttttt atgtacaata ggtttttcag tagatataaa
     7201 agagttttta ggttcaactt ttactataac actttactgt tttagcataa tttttgcttc
     7261 atttgttttc catttaggca taactagact tttaaagata aagtatcaat atgtaatact
     7321 ttctatagta ggagcaattg ctgatggtcc tacttcagct ttagtagctg cttctgcaaa
     7381 gtggaattcg cttgtaagtg tagcagtcgt aatgggagta attggtggag tattaggaaa
     7441 ttatgcaggt atatcagtgg catatgcaat aaagatgtct ctaggattat aggggggagt
     7501 ttaattgata aaatttaaat tatacatatc tggactatac agtggaaatg taatctttga
     7561 tggagattta ttaattgaaa agttgaatcc ttttacaaat aaagtagaaa atttaaaacc
     7621 aataagcaaa gaagaaaata catattattt aaatcttaca aaaatagatt taaagtcttt
     7681 atttaataac tttgatgtat atactaaaag cttagtaaac acagataaag acacagttat
     7741 aaataactta ggagaaacgc attctaaact taatgaatat atatgggttc aaagaaacaa
     7801 aaagttccca ctagatataa ttattgtaga taataaaata gtaggtttta tctgtttatc
     7861 tagagaaact tgtactatat taattatgga tggatatgaa gagtatactg tattaaaaga
     7921 atgggaaaaa actcataaaa atgaagagat atattctata agatttggtg gaaattatat
     7981 gattgatatg aaagatggta taaaactttc tacagatgta tatctccctg attttgtaga
     8041 ttctaccaaa aaagcaccaa caattcttat gagaactcca tatggaaaag aaaatgataa
     8101 agaaatatat tataaatatg tacaaagagg atatgctgtt gtaatacaag atgttagggg
     8161 aagaaatgaa tcagaaggta agtgggaacc tatgattcat gagagggaag atggagatag
     8221 taccataaat tggatagtat cacaggaatg gtcaagtgga atagttggaa tgcttggagc
     8281 atcatatctt ggttatgtcc aatgggcagc tgcatcttca ggcaataagc atcttaaagc
     8341 tttagttagt atagtaacat caggaagccc ttttattgat ataccaagaa aaggaggagc
     8401 ttttgtatct ggtatgcttg catgggcatt tatggtatca agaaataaag ttgatagaag
     8461 caaaatggta agagatgatt gggatgatgt tttaaacata cgaccaatag aaaatatacc
     8521 ttttgaagct cttggatata gaattgaatt tctggaagaa tggttaaaga gagtagaaaa
     8581 agatgagtat tgggatttga tggattggca tttacaaaaa gataaaataa atgtaccttc
     8641 attggttgta tctgggtggt atgatgataa tagtatggga actacagagg ccttagatgt
     8701 tataaaggac tatgaaaaag gaaagagaaa ggctattcta ggtccatgga tgcataattc
     8761 aaatacttta agagatataa atggtatttc tttaggaaat agttcattaa gatatgattt
     8821 ggattataat tatctattat ggtttgataa atacttaaaa ggtatagaaa ataacataga
     8881 tacgactgct ccagtagagt attactctgt aggttctaat aagtggaaga cagaagaaaa
     8941 ttggccaata ataaataaga tagataaaag tatgtatcta ataagtgatg gaaatgctaa
     9001 cacatcttta ggaaatggta gattagtatt tgataatgat ttagaagaaa agtatgatag
     9061 ttatatatat aatccaaaag acccatcagt acaactaatt gatatgtcag agaatgaagt
     9121 tggagttcca aataactaca aagatttaga aaaaagaagt gatatgttgt gttatacatc
     9181 agatgctttt agtgaagaat ttactgtaac aggtgatata aagttagagt tttttgcatc
     9241 aagttctgcc aaagatactg actgggttat aaaaattatg gacgtggatt tagatggaaa
     9301 ttcaattaaa ttagctgatg gtatacttag tgctagattt agaaatagtt tttataagtc
     9361 agagtttatg gaagaaggag agatttataa gtttacgatt ataacttcaa aaatatcaaa
     9421 cacgtttaaa gttggtcata aaattagact agatataacg tctagtgcta aaaactttat
     9481 tttccaaaat agcaacacga cagagggata taatagtatt gaatatatag aagcaaagaa
     9541 tacgatttat catggaggta aatatccatc taaattgata ttgccaatag agaataagta
     9601 aatttatata gattaaatag tgaggaggtg tctcaaaatg aattttttag ttcgtgagac
     9661 actttctttt atacaaaatc aaatgtattt tcatcatttc aacaatgaaa aagaggctta
     9721 tctctctgtt ttgagatagc ctcctataag ctaaattgat tattttgtag ctgcaacaac
     9781 gctcatttca actaacaaat gctctctagc aagtctagct tcaacacaag ctctagcagg
     9841 ttcaaatcct ttttcaaccc aagcatccca aacagagttc atagcttcaa agtctttcat
     9901 atctcttaaa taaatagtaa cagatagcaa atgttgctta tctgaaccat atttgtttaa
     9961 taaatcttct attttagcta aaacttcagt tgtttgttca attactcctc cttcagcatg
    10021 agtttgtcca cataaataaa ctgtattgtt atgaactaca gctctactca ttcttccagt
    10081 tccttcatat ctctttattt ccatatttta aatacctcca tagttaaaaa atctattaaa
    10141 aatctaaata ataaattaaa aaaagtctat actatataaa attatattat atattctgat
    10201 aataatccat aaaaacatat aactttttag ctataattat tattgttatt aattgaaaag
    10261 atttattagt tacttgacat agtttacact tttaattata gtataaattg gaatatttag
    10321 ataattatta tttaatgtat aagctaagtt tttaattaat atggctttat acatttatat
    10381 agaaaagagg tacgtatgag tagcacaatc aaatcaaatc gaataaaagg tataatgttt
    10441 ataatagcat cagcgcttgg ctttgcaatg atgtctgcat tcgtaaagct agctggagat
    10501 ttaccaagtt ttcaaaaagt gttctttaga aatctggtat cagctataat agctttttgg
    10561 cttatcataa aacataaagg aagtctaact ggtaaaaaag aaaatttaaa agtactttta
    10621 tataggtcaa tatttggaac tctaggagtt atatttaact actatgcaat tgacaagttg
    10681 atgctttcag atgctaatat gctaaataaa atgagtccct tccttgtagt aatattttgt
    10741 gcaattgctc taaaagaaaa aataaatgta aagcagatag gagctattat aattgcattt
    10801 ataggagctt tatttataat aaaaccaaca tttagtgtag aagtaattcc atatcttgga
    10861 ggaattgcag gagctgtatt tgcagcaatg gcatatactt gtttaagagt tcttggagat
    10921 agagaagatt actatacaat tgtatttttc ttttcagttt tttcattagt tactgtaggg
    10981 ccaattgctt ttgcagtata tgaaccaatg acccttatgc aacttatata tctattacta
    11041 ggtggagtat ttgcaagtct aggtcaattt ggaataactc tagcatataa atatgcacct
    11101 gctaaggaaa tctctatatt tgactatagt aatataatct tttcagcaat tttaagtata
    11161 ttcttattca atgtctatcc agatatgtta agtgttgttg gatatttaat agtattttcg
    11221 gcagcatttt atatgttttt atacaataag aagttagata aattagacaa agaaaagaag
    11281 aagtaaacta gataaaagtg tttttataat ttgtattgaa tatttaagtc tttgtattaa
    11341 aattaaagaa aatttaatta aaaatgtttg gtatattaac ttataacagc agttaaaatt
    11401 gaaaaattat aactgctgtt tcttttatta aaaatatcca aatgtctgta aaaatagact
    11461 ataatagagt agtataaata atataaaggg gataaattaa agtatggaaa aagtgaaagt
    11521 tgctgtgatt ggagcaggta atagaggaac gtatgcatat gctccatata tttatgaaaa
    11581 ttctgatgta tgtgaaatag ttgctgtagc agaacctaaa aaaggaagac gagaactatt
    11641 tacacaaaaa tataacctag atagtaaaaa tgtatttgaa actttagaag acttttttaa
    11701 acatgacaag atggcagatg cagtaataat agctacaaat gatgatagac attatgatgt
    11761 agcaaaacta gctcttgaaa aaggttatca tgtactatta gaaaagccaa tgtctaatag
    11821 tttagatggt ttggtacata tagatgactt gtgtgataag tataaagata agatttttat
    11881 gatttgccat gtactgagat attctccatt ttataataaa ttaaaagaaa ttgtagaaag
    11941 caaaaaatta ggagagctag ttagtataca atataatgaa aatataggat attggcattt
    12001 tgcacatagc tttactagag ggaattggag aaatagcaat gaaaccagcc ctttaatttt
    12061 agcaaagagt tgtcatgaca tggatatttt gttatattta gtaggaagta gatgcaaaaa
    12121 aatatctgca tttggaagct taaagcatct taataaccaa aatgctagtg gagagatggc
    12181 acaaaattgt cttcagtgtt tagttgaaaa aaaatgtcca tattcagcaa agagaattta
    12241 tttagaaaaa gatagaagta taaatagagc tgttcacata aatccaactg aagagaatct
    12301 attaaatata cttaagacaa gtccatatgg aagatgtgtt tacagatgtg ataataatgt
    12361 tgtagacaat atggtaaata tactacagtt tgaaaatgga gtgacggtta cttttaattt
    12421 atgtgcattt actaaagaaa atggaagaac tataaagtta atgtttagcc atggtgaagt
    12481 tggtggggat ttaaataaaa atgagataag aattaaagaa tttggaaaaa acgaagaaat
    12541 agttatgaat ccatcaaatc aacagaatat ggtagaggaa tatgatagaa atcttatagc
    12601 tgaatttatt aaattagttt caaataaaga actagaaaag ggtagagtag cagcaaaaga
    12661 agctatacaa agtcatgtaa tggcatttgc agcagaatat tctagggtca gtgatgaagt
    12721 agtgtatata gaagaatttt ttgatagtgc taagcaaatg actaaggaga tagaagaaac
    12781 tatattttaa ttaataaaaa tttattaaat aaaataaaga tagattaaat aatagaaata
    12841 cgaaaatatg tgttctatta aggaatctat cttttttatt aaggtttttt cttatcccta
    12901 tcaaaaaaat ccatacattc tgataaaaga taagaaagta aagcgaataa gatttctgta
    12961 cttaaaggaa tcgttactga atccataaag gtatcaatta tgttcatagc tagaaaagtt
    13021 agtataaaat caattataaa ttcgcaaagt ttatatattg agtatggtaa tttgtttaca
    13081 tatctgaata tatctaaaat agtagtgcat agaaaatcaa ttggagtggt tatacaaaaa
    13141 tatattaaaa agaaaattaa tacagctcca agagatttat attcaaatcc taagaatgat
    13201 aagaagccaa ttcctataac cgtgaaaata caaaaagtta taccaaaaaa aacagcaaaa
    13261 aataaaaata cagaaataaa ttcgcctaaa ggaatccttc ttttattact tatcttaatc
    13321 aactccttaa tttatgatat gaaatttata taaataataa cattcttaaa aattcataat
    13381 agtcctctgt ttcaaatagt actgtttgag aatctatttt tttcatattt ggataaaaag
    13441 aggccttaaa agctttataa tgttctctaa attttatttc tatcttaaat ttatctggca
    13501 attttattaa gtgcctagat aaatctccag ataaagcttt tttgacacct ttttcaattt
    13561 tttttagtgc aaggtttgga tggattgaaa ttgaaccatt tccaattcct tttgacacag
    13621 gaacagtgac tatatttgga tttagttttt ttgcactctc acaaagcatt tcatctccac
    13681 ttaaaaatgc tacaggaaca ttataatatg cagcaatata agcatttata gtaaattcag
    13741 atgctatttc atcatttatt ttaaaatagt cataatcaat atccatagta tgggaaagtg
    13801 gattaccatt acaagtagct ccagagtgat agccagtgaa tatacatgca tcaaagcttt
    13861 catcaattcc agccatcata actagtgggt ctttggtcca tcctctaagt attttgacgt
    13921 tttcaggtaa aatatttgga ttaatatttc taccactgtc atgtgcatct ttaacaagta
    13981 tttcatgtac accagattga gtagctccat cacatgctgc tgttatctct ttactcattt
    14041 gtgttctata gtgttgcgaa aattcacatt caagttttgt ttcatcccaa tttacaatac
    14101 cacaagtacc ttctatatca gcacttaaat acaatttcat aatatatacc cccttatgtt
    14161 tattattgat gataataaca gataacagaa aatacataat caataaaggg cataaaaata
    14221 ttatgtctta ctattaacta ttattaaatc tgtaactgaa aaataactaa attggtataa
    14281 aaaaatataa cactatgtgt c
//
LOCUS       DAELJO010000087        14248 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELJO010000087 DAELJO010000000
VERSION     DAELJO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.087"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(33..524)
                     /locus_tag="KPB88_002202"
     CDS             complement(33..524)
                     /locus_tag="KPB88_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054476.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFNSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(535..978)
                     /locus_tag="KPB88_002203"
     CDS             complement(535..978)
                     /locus_tag="KPB88_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054477.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(968..1807)
                     /locus_tag="KPB88_002204"
     CDS             complement(968..1807)
                     /locus_tag="KPB88_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054478.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLNLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(2025..3410)
                     /locus_tag="KPB88_002205"
     CDS             complement(2025..3410)
                     /locus_tag="KPB88_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0054479.1"
                     /translation="MDDLNKKFLIEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKHGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDGLSQKVSTIKESIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPLRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMKHVRDVFGRIVDIA
                     KGAALMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(3582..4325)
                     /locus_tag="KPB88_002206"
     CDS             complement(3582..4325)
                     /locus_tag="KPB88_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG0054480.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPLVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVSMLDWLTC"
     gene            complement(4345..5607)
                     /locus_tag="KPB88_002207"
     CDS             complement(4345..5607)
                     /locus_tag="KPB88_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0054481.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYCATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMIRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(5791..6789)
                     /locus_tag="KPB88_002208"
     CDS             complement(5791..6789)
                     /locus_tag="KPB88_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG0054482.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(6773..8284)
                     /locus_tag="KPB88_002209"
     CDS             complement(6773..8284)
                     /locus_tag="KPB88_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0054483.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVESLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMIENNVLKLAFGGKINEFNN"
     gene            complement(8338..9300)
                     /locus_tag="KPB88_002210"
     CDS             complement(8338..9300)
                     /locus_tag="KPB88_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0054484.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(9348..10238)
                     /gene="rbsK"
                     /locus_tag="KPB88_002211"
     CDS             complement(9348..10238)
                     /gene="rbsK"
                     /locus_tag="KPB88_002211"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG0054485.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(10280..11293)
                     /locus_tag="KPB88_002212"
     CDS             complement(10280..11293)
                     /locus_tag="KPB88_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0054486.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(11522..12499)
                     /gene="bioB"
                     /locus_tag="KPB88_002213"
     CDS             complement(11522..12499)
                     /gene="bioB"
                     /locus_tag="KPB88_002213"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG0054487.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(12705..12968)
                     /locus_tag="KPB88_002214"
     CDS             complement(12705..12968)
                     /locus_tag="KPB88_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054488.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMENLDGVKTRIVL"
     gene            complement(13038..14111)
                     /locus_tag="KPB88_002215"
     CDS             complement(13038..14111)
                     /locus_tag="KPB88_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0054489.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
BASE COUNT         4361 a         2734 c         1403 g         5750 t
ORIGIN      
        1 atttcattaa atttaaagat aaaaataagc aattattttt taaatagcat ctttcttaac
       61 ctattattgt aataacctat aaaagaactg taaacatagt catatactat aaaggctatc
      121 tcaaatgaac ctataataaa tatgtttatt ggaatataaa ctattgttct taataaaaaa
      181 tacaatataa gtatcattat atttgcaaat actaatttca taaaatactc aatatatata
      241 gacctatctt tttctataag atattttaca ataccatata ttccaaaggt aagtgaatat
      301 acaatccatt gagacttact tgccatcact ataaatccta aaactataga acctatataa
      361 aaagcaattc cactattaaa gccccattcc attattacaa ttgatactaa aagtgatgct
      421 aaccccataa taaacaatgt atttacagga ataatatttg taagagtcaa caatattaca
      481 tttaatgcta ataaaactcc tccataagca acttttatac tcatatttat catcctaaca
      541 acaagttatt aaatctccac caaaacattc acaacatgaa tctaaacacc aaagtttagc
      601 acaattttca caacaatcgt cacctagaca acaacagcaa tcatcacagc aacagcaaca
      661 acatccatct aaatcattac gtcttcttct attataattg tcagccctat tgctataatc
      721 atttctatat tgtgtataac ttctaagtgc atcactatat tcactatttt caggctccat
      781 aaattttgct ctttttaaga agtcttcacc ttcttcataa tatcctatgt tcattgcaga
      841 aaagccagtt aaataatacc actctgcaca tttgtcagtc aaagttttta aaaaatcata
      901 tgctttttta aattcactat tttctatgaa tgctcttgct ctcataaaat tttcattatt
      961 ataagtatta ctgtacattc tttccacctc tactttctaa tatattaata tatcttaaat
     1021 aaacaccaga ataaattata ttttctatta ttccaacatt tttatttaga tttagattat
     1081 caattcccct agctaaaaac cctaatgatg ttactattat tttttttact ttttctttta
     1141 actcatcatt tttatcaata tactctataa atggattata tcttcctttc ttaaaatctt
     1201 catctaaatc ttcataggca tctagtagat atatatactt acctatatta aaacctatca
     1261 ttcttaagtt tctctcattt tcatcttttt tatagacaaa tacttcaccc attatatttc
     1321 caaatatact agatacttca tctatattta tatttttgtc tttttctaac ttatttaatt
     1381 catttagttg ttgttttata tactctgctt tttcaggata cttttcatat gctaaattta
     1441 atttgttttt atatatataa tatgccaatt tatcttttat gcgtttgtca tccattaaat
     1501 tatcttccaa tttataatac gttaaaagaa tattcatact tgctgcatat tctgtaatat
     1561 catttgtaat tacctttttc tttttaaatg gatttactat acacacttcc tctgtactta
     1621 tagactctgg gttatatact gctgttaata aaacaattaa aaatgttata tcataattca
     1681 aagaaaacct agagatttct ccatgatttc tttttaagta tttacatagt ccacaatagt
     1741 aagctttgta ataatcatat tctctaaatg ttaaatccat tttgtttatt ttaacatatc
     1801 caaacataaa tactcctttt tgtactattt tttatttaga ttatatatag tataacactt
     1861 tttatcaaga ttatataatt tacagtattc taagaaactt aaaaaattcc ttctaatcat
     1921 tgtatcttaa atttaaaata attagaaggt tggttaaaaa taatattaaa ttatatactt
     1981 atattaactc atacaagtta gttatgctta tataaattta agttttaaat aattggtata
     2041 tatttaaaac tacctacagt atccttcaat tctttttttg ctttatttat aaccaattca
     2101 tcaagcatta catcatttat tgaacatgat atagatttag atgccaataa cattgcttta
     2161 aatcccatat cagaacctgc actggcacaa gattgccatg tatgggcaga aataccaata
     2221 ggccaaactg aacaagagca ttgtgctgta ggaattatgt agctaacatc tccaatatcc
     2281 aatgataatg atatacagtt attatgagta aatgtatcat ctgtaacctc atcatgtaaa
     2341 ttcatcttag taatatcctt atttccactt aatacactag aaacactact tcttttatct
     2401 atagacactg tctcagctaa tttatctgca aaatagtagt catcttcatt atagttttgc
     2461 acacctacta atttcatatt ttgtgataga atatctgtta atattgtatt tggaatatag
     2521 tcatatactc cacttataat atcatattcc atactagtac cagtcattaa tgctgctcct
     2581 tttgctatat caacaatcct accaaataca tctcttacat gcttcatttt aactcctctt
     2641 atgtaatacc acacttctgc aaaatctgga actatatttg gcatttttcc accattagta
     2701 gttacatagt gtactctaca actatcaaat atatgttctc tcaaataatt acaacctata
     2761 ttcataagtt ctactgcatc aagtgcactt cttccgttat aaggagcttg tgctgcatga
     2821 gcactaatcc cattatatct aaattttaca gagtaattag caagtgttgg gattcttaaa
     2881 ggagtattta tatcaaatgg atgccaacta aaagcacaat caattccatc aaaaaaacct
     2941 ttttttatca tcaatacttt tccagataaa tcttcctctg ctggacatcc atagtatttt
     3001 atagtccctt tcagcttttt tagtttgatt aattccttta ctgcaattac agctcctatt
     3061 gaacctgtac ccagtaagtt atgtccacat ccatgacctg caccaccatc tacaatagat
     3121 tctttaatag tggatacttt ttgagacaaa ccatctaaag catcatattc tcccaaaact
     3181 gctataactg gtctaccttc tccatagcta gccgcaaatg ctgtatccat accttctaat
     3241 tcatctatac taaaaccatg tttttttaag tattcttttt gtaaatttga agcataatat
     3301 tctttatact gaagttctgg atttttccat atcttatctg atatatctct taattctccc
     3361 tgtatctcat ctatatattc tattaaaaac tttttattta aatcatccat attaattata
     3421 taaacctccc tgtttctaat aacttatcga ttatcagcca aaatgactaa ttttgactga
     3481 tttaaaaata ttttctacgt acaaccattt tatacaataa aacctcttga cttgtaagtt
     3541 tcattttatc aagtaagagg ttttcaagtt ttataaagtt tttagcatgt taaccagtca
     3601 agcatactaa cccataattt attatagtat ttccaatcag taaattcttt tgggccccag
     3661 tgaggtgcac agtcagaact aaagatagca gattttcctt ttccaaactc accaacagct
     3721 accaatgggt ctcctccaat agttgctaac acagggcaat tatctctagc aactgttttg
     3781 ttatatccta aaaactttgg ccattctgtt ggtatacctt ttaatactgg atgttcagag
     3841 tcaataactt ctggaattat tcctgctggt ttttcaactc tatcatcttt atctatcatt
     3901 gttattggta atacatcttt gatagcagtt tctccaaatc tagcttttgc atctattcct
     3961 gtaaatgaca tatatccacc taccatgatt aacgccccac cattatttac atactctttg
     4021 attagttcaa gtctatctgg attactattg caatctataa aagtactatt agataataag
     4081 aacgtattag aacctatatc agataatatt atacagtcat actcatttaa ctcttctaat
     4141 ttatacggaa aactatctgc tgctacatgt gcaggcatat gaactgtctc atatcctcca
     4201 gattctattg cttctttcaa ccactttaca gcttcttcat aatgagttgt aacaaacgta
     4261 tcaaatcctt ttatatgagt tatatttttt acccaagatt cccctactat taatactttt
     4321 ttcatttata tattcctcct acttttataa gtttaatatt agacttgcat acataagtat
     4381 actatctaaa tactccttaa tttcaacata ttcatcaatt gaatgacatt gttctattga
     4441 acctggccct aatataagtg ttggtatttc agctaaattt ttcattaatc tcgcatcatt
     4501 gccagctgtt gaacctttta tttctaattt tttacctaga gtttcttcta gtattttatg
     4561 tgcattcact acaaaatcag aatccttgtc catctcaaaa cctaaccctg attgatatat
     4621 gtttatattt ggtttattat ctttaagcca taaatctcca ttagccctta tcattatgac
     4681 atttgttaca tcatttacaa cttgttcata actcattata tttggcaaga agtgtacaca
     4741 taagttaaat acacacttat ctggcacagt agagcctgct gttccaccat ttattacacc
     4801 taaatttatt gtaggtgaag gtaatagtgg atgcttatat atcataagcc agttatgttc
     4861 caactctttt atatcttgaa gcaacaacat agccttctca attgcattta caccctccca
     4921 cttactacca cagtgaagag acacaccttt aacctcaact tcaaagaata caaatcccat
     4981 atgtgcaact attaagtttt gttctgatgg ttcacaaatt atgcaacaat ctccatcaat
     5041 accattcata actgcattta tggtaccatt tccgccacct tcttcatcta ctactgacat
     5101 tatttttaca ttaccaggta catttagacc actatctttt ataagcttta cagctaatat
     5161 tgatgctata agcccagatt tcatatcagc agttccaaga ccataaagtt ttccattatc
     5221 ttctgtagca caatatggat tatatttcca cttacttata tcacctggtg gcatagtgtc
     5281 tacatgacca ttaaatacaa ttgtcttacc aggtaaatca tcactatact tacaaattaa
     5341 attatatcta tcttgataat tatgacctag atttccttct ttatatatat tttttgcttt
     5401 tttaattaat tcttcgctca tttcttgtct atctacacta aaacctatgt aatttgcaag
     5461 tttttcaata tattcctgtc cttctttttc aaatccacct agtattccat gaccaacatc
     5521 ttcagtcttt atacttacaa gttcttttaa ataatctatg tattcttgtt ttttactatt
     5581 taatgtatct aagagaattt ttttcatatt tcctcctaag atttattttt acatttaaaa
     5641 tttcaagtct aatcttcatc tatttaagcc atagtaaact ttttcactta gcatattccc
     5701 atttaatgta taaagatata tgcaaattaa agtataaaaa tatatacaaa acttaataat
     5761 ttttatagtt tataagctaa aatttaatta ctatttagat ttagctgatt tttttaattt
     5821 tatattattt atccatatag caaataacat taccaaacct tttataatca tttgattata
     5881 tgaagataca cttaataagt ttaatccatt tcctaatatt cctattattc caacacctat
     5941 acatgtccct actatagaac cagaaccacc catcatatta gtgccaccaa gcacaacagc
     6001 tgctatagca tccaattctg ctccatctcc aacagttggt gaacctgatg ccaaacgtga
     6061 tgatagtatt atcccagcta gtgctgttag tagtccagct agtgcgtata cttttactat
     6121 aactttattt acttttactc cacataatct agcggcctct tcatttccac ctactgcatg
     6181 tacatgacgt ccaaaattag ttctttttaa tagaaaatgt cctgctatta caactataat
     6241 cataattata actggtattg gtattggtcc aatatatcct ctaccgagca tcttaaatgc
     6301 atctgtattt actgatatag gtcttcctcc tgtaaataca aagcttgccc cacttattat
     6361 cgttaacatg gctaaagtta caatgaatgg aggtagtttt tgtgttgaaa taattgcacc
     6421 ttttatgact cctaatattg tccctattgc taatgcaagt atcataccta cccatatagg
     6481 tgcattataa ttcactactg ttgtagccca aaatgtacct actaaagcaa ttactgctcc
     6541 tacacttaaa tctattcctc ctgtaattat tacaaatgtc atacctactg caagtattgc
     6601 atttattgat atttgtcttc caacattaat taaattatct aaagttaaaa agttagatga
     6661 agctacactc attattataa aaattattac aagtcctaca gctgcactcc cttggtctct
     6721 aaatacactt gttaattttg tcaataatgg agaagatttt tcttctttat gcttagttat
     6781 tgaactcatt aattttacct ccgaaagcca attttaatac attattttca atcatttctt
     6841 caccttctaa aaatccactt atatgacctt ctctcataac cattattctg tcactcattg
     6901 atattaattc tggtatttca gaagatatca ttattatact tacaccactc tccactaaat
     6961 ctcccattag attgtatatc tctgccttag catttacatc tatacctctg gttggttcat
     7021 ccataatcaa tatatctggt tgtaataaaa gccatcttgc tattatagct ttttgttgat
     7081 ttccaccact taaactttgt ataacttgat tcatatgtgg tgtttttatc tttaaagctt
     7141 ctatgtattt ttctaaaatc tctttttcct tttttctatc tataattgat ttaaacttag
     7201 atatcttttt taacccagcc atagttgtgt tttctttgac actcatttgt aaaaacaatc
     7261 cagtaccttt tctatcttct gttattaatc ctagtccatt ttctattgca tctgatggag
     7321 ttttaaactt tacttcttca ttgtttaaat atattttccc agaatcatat gttctaagtc
     7381 caaatatagc ttctactatt tcacttctct tacttccaac tagtccacct attcccaata
     7441 tttcaccttt tcttatatta aagctaatat cattaaaaac attgttctta gttagacttt
     7501 ccactcgcat aatttcttct tttatttcta cttttctttt tgggaaaaat tggtctagct
     7561 ttctatctat cattaaatta actaattctt cctcagacga tacatctttg gtatttaaag
     7621 tcgaaatcgt tttcccatct cgcattatag ttattctgtc acaaatctca aagagttctt
     7681 ccatcctatg ggatatatat attatactaa ctccactctc ttttaaatca tttataagtc
     7741 taaaaagtat ttcagtctct tttttactta ttgaagaagt aggttcatcc attattaata
     7801 ctttagtttc tgatgataaa actcttgcta tttctaccat ttgtttttga gctatactaa
     7861 gctctttgac taaactattt ggatttatat ctattcccat attatcgaaa atctttttag
     7921 cctcttcatt catttttttg tcattggtta agactttgcc atttatttct tttcccataa
     7981 aaatattttg tgctactgtt aattcgtcaa aaactgttag ttcttgatat atggcattta
     8041 tacctatctt tttagagata tctggggata tattctctat ttctttgcct tcaaaaataa
     8101 attttccaga agttttcgaa tgtgccccag ttaaaatctt tatcattgtt gatttaccag
     8161 caccattttc acccagtagt gcatgaactt cgccatgaaa taactcaaaa ttaacattat
     8221 ctaatgctct gacaccagga aattctttag caatattact taacttcaga attatattac
     8281 tcataatgac actccttttt aaataggatt tctttcaaat aatctcattt tattttacta
     8341 attcttaaag tcttttatat tttccttatc ataaagtaaa gctggtactg gtatattctt
     8401 ttctaccttt tcccctttta tagcttttac tactgcttct acagctaaac gtcccatctc
     8461 tttaggttgt tgttgaatca tagcaagaat agttccatct gttattccac ttgatgcttc
     8521 ttcagttaag tcaaatccta caatcttaac atccttattt tttgcagatt caagtgcagc
     8581 tttagctcct agtacactat tttcagctgt tgcatatatt atatctacat ctggattaga
     8641 ttgaattaag ttttctactg tattaagaga ttcttctctg tcatatcctg gttgtgaatt
     8701 taatatctta acatttgctg aaccttttaa tgcatcttta aatccatcta ttctctgcat
     8761 ttgtatttga gattttaaat ctgtcactat tgctatttct gactttccac ctaacttttc
     8821 atctatgtat tttttagtgt attcccctaa ttttttacca gcatcataac taactgttcc
     8881 tacataactt gtaatatttt cagtatttaa tacagcatct actgtaacta ctggcatttt
     8941 ttcaaatgct ccttctactg ctggtactat agccgaatta tcagttccaa gtactatcat
     9001 tccatctaca ttctgttgta taaaatcttc tattgctgat gtttgcttag ctacatcttg
     9061 gtctgggtca tttactataa ctttcacacc taattcttta gctttttctt ttattccctc
     9121 atcaatatct ataaaaaatt gatgttgaag agttattgta gatactccaa tagttaaatc
     9181 tttcttgtct gaaccacctt tattatctga tttacttggt ccttccattg aacaccctgt
     9241 taacattcct attgctagta ttccagcaat tcctaaagat aaaatttttc tcactctcat
     9301 aattaacctc catttttaat agttttaaat ttgaacaaat tagattttta tttattattt
     9361 aaatattttt caacatcttc tttataaggc ataccagatt gtgcacctag tttagttaca
     9421 gaaagacctg atactactac tgcatattct actgctgtat ataaattttt cccttctgtt
     9481 atagccactg ccaatgctcc attaaatgta tctcctgctc ctgtggtatc ttgaacatca
     9541 acacttatac aagatacatg cttgatttcc tcaccatcat aaaatctagc tccatttttt
     9601 ccttctgtta ttacaagttt atttggatat ttttttaata cttcttctat cccttcattt
     9661 gtgtcaaaaa ctatcttata ttcatgttca tttggtgtta agtaagttac tttttctatt
     9721 atatcttcat ttagttttac tgctggtgct ggatttaata aaaccctaat cctattttca
     9781 aaacagaaat tcactacata atttattgtt tttaatggaa tctctaattg tagcaatact
     9841 atatctgcat tctttatctc ttcttcattc tttttaatta attctatatc tactagctca
     9901 ttagtccctg gtacaacaac aatactatta tcattttcac ataatgttat aacagcaaca
     9961 ccagaacttt tgtgattatt tctggatact aagctataat caactttatt cgttctaaaa
    10021 tttcttatta actcctctcc taagccatct tcccctatgc atgatatcat ttttacattt
    10081 gctcctagtt tagaagctga gatagcttga ttagcccctt ttcctccagg tgatgtaaaa
    10141 aaactattac ctaatactgt ttctcctttt tctggtcttt tatctacaga acataccatg
    10201 tccatgttta cacttcctat gacaactatg tttcccatta cattactcct ttacatatct
    10261 atttatgtct ctcgtgctgt tattcacttt cttttgttga atctcttatg accaattctg
    10321 gtttgaaaat caattcatca aacgattctt ttttatctcc tcttaaacaa tttatcaata
    10381 tttcactggc tttatatcct atatcataag acggctgttt tactgtggta agagaagggc
    10441 ttattagact tgatgtttga atatcatcaa atcccacgac acttatatca tttggtatac
    10501 ttaatcctct ttctttaaga cctcttatga cacctattgc aatcaaatca tttgcacaaa
    10561 atattgcatc aaaatctatc ttttctaaat tttttataaa ttcttgtccc cattcaatct
    10621 tatattttcc ttcaaataca taagcaggat tgtagcttaa attgcactcg attagagcct
    10681 ttttatatcc ttctaatcta tctactgcta tttcattttg aagtggacca cttaggtata
    10741 ttattttctt atttccaata tctaataaat gttttgtagc tatataggcc ccttcaaagt
    10801 tatttatttt aacaattcct tttaaggttt ctttgttcat acttatcttc ttatcaatca
    10861 agacagttgg tatttttata tctttataat ttgcttcttt ccagtttgta tttgaagatg
    10921 ctgcaaatat aattccatct atcatcctct ctgatagtgt tcttattcct tcatattctt
    10981 ttttagcatc ttcatctgta ttacataaaa ttatattgta accttcttca ttagctttgt
    11041 cttctacacc tcttgataca tctgtaaaga atggatttcg tatatctggt ataagaagcc
    11101 ctatagtatt agttttttta gttactaaac tttgtgctaa tttatttggt acataatttt
    11161 tttccttcat tattttcaaa attttttctc ttgttgcctg acttatattt tcatctttat
    11221 tattaatgac ttttgacaca gttgttttag aaacaccagc caattcgcca atttctttta
    11281 tagttatact caagaagttc acccccaaaa tttaggaaac cgttttactt aaccggttac
    11341 ctagatacta tcacatcttt tttccatttg caaccatttt atataaattt tgatattttt
    11401 cccttttttc aaaatattgc aacttataat ttaattttaa tatttaaatt aatctgcaaa
    11461 aaaaaattaa tatactttta taattatcta aaagtatatt aattaattta agcagtattt
    11521 attaaagttc aaatccttgc tctataatca tttttttatc attttcaatc ccacttccaa
    11581 cagttgtcaa ataatcacca acaattgcag aatttactcc cccattaaat ccaactttgt
    11641 catatccctt taatgatagc cttccaccag cataccttat gtacacatta ggaattataa
    11701 acctaaatac tgctgttgtt tttagtattt ccattggttc taatacatca taattttcca
    11761 ttggtgtatt cttaatagga tttaaaatgt ttattggaaa tgattttaca tttaattctc
    11821 gtatttcaaa agccattttt agtctctgtt cttcactctc attcattcca attataccgc
    11881 ctacacatac atctagccca gcttttttag catcttttat agtttttatc ctatctttat
    11941 atgtgtgtgt tgtacatata ttatgaaaat tatctctaca cgtctcaaca ttgtgatgat
    12001 atgtagtcac acctgctgac ttcaacattt tagcttgctc ataatctata atacctaaag
    12061 atgcacataa ttttaaatta gtattttccc tcaatccttc atagatattt aaaatattat
    12121 taaactcctt tcctgacata cctttaccac ttgtaaccaa tgagaatcta tgcactcctt
    12181 tactctccat ctctttagct ctatttaaga tttcattgta atctaataat gaatattctt
    12241 ctacaccagt tttataataa gaagactgag cacaaaattt acagtcttca ctacatttgc
    12301 cagatttagc attcattatc gtacataaat ctgcttttct tcccataaaa tattctctta
    12361 tttcatttgc agattttaat agaatatcaa aatcgttttt attatttgta tcaaggctta
    12421 ttaaattaag agcttcttca taacttattt ctttttctct caatacttta ttttttaatt
    12481 ttattatgta tttttccata tatcctccta tttgtcaact taatattttt atgtagttta
    12541 cttattatat atagtattgc atatataaca tttattggtc aactttattt tagacacaag
    12601 tttactatac acaaaaaaat actgcttcaa aacaatatta gtatatagat tatacatatt
    12661 attattaaag cagtattttt tattagaata taattcttac tggtttataa aactatcctt
    12721 gtcttaactc catccaagtt ttccattgta ataacttcta agccttcata ttctctaaat
    12781 agccatttta aatccttcat ggcttgatta acctgacttt ttgttagatt atttataaaa
    12841 tattcttttc tagtttcctc atactctttg ctagtcaaaa agtttcttag aaaattaaac
    12901 gcaaatacac ttggaatatt gtttacatta gccttcttgc tgttttcctt ctcatagtaa
    12961 attttcataa atgcatactc ctttgatact tttagatttc tcttacaata attatatgca
    13021 tttaagtgct actattctta taattttatt aattcatatt ctttacttcc catacccatt
    13081 tcacatgcat agtctattat tctagttcca tttacatgag gatgttcatg tttgaaaaca
    13141 tcatgaccca tttccttaca aatcaaatca tagcatgctt gttcaatagc tacagggtct
    13201 gttgatgcta atattcctat atcatgggca attggtctac ctgaccaagg tacacaatca
    13261 caaagtggag ttacattcat aacaaaagtt atatacccta ccttgtcttt tttatttgat
    13321 actgctccat aagcgtattc tgccatcttt tcgacaaaca catctgagtc actttcccat
    13381 tgtatagtta ctgctctagt tggacagaca gttggacatt ctccacatcc ataacataca
    13441 tcagaatcta taactgcttt tttatccact atacttatag cttttgtagg acaagaatta
    13501 acacactttc cacaacctac acacttcttt tcctttacta ctggagtaac atcagaatgt
    13561 tgcatttgtt ttcctgctgc acttgcacat cccatagcta agttttttaa tgctcctcca
    13621 aaacctgctg cttcatgacc tttaaaatga ctcattacta tcattgcaga agaattataa
    13681 atttctccac ctatttttac actttcaaaa tgttttttgt ctatttttac attctcataa
    13741 tttctactat ataatccatc tgcaattatt actggtgcat ttacaactgc ataagcaaat
    13801 ccattttcta tagcagttgt aagatggtct acagaatttg ttctactccc tgtatacagt
    13861 gtatttgtat ctgttaaaaa tggctttcct tcgcagtctt taaccttatc aaccacttgt
    13921 ctaactgcca caggactcat atatgtagta tttccctttt ctccaaagtg aagtttaatt
    13981 gcaacttggt cattcttttc tattaaatct ttgaatcctg cttcatcaaa taatcttctt
    14041 acattgtttg gtacgttttt attttgcgaa tttgagtata aatcacaaaa atatactttt
    14101 gacttttcca tatctacacc cctttaaaat atttacttat actaatttta tcacttttaa
    14161 agtaattttt agtatatttt accataatta aatattctta tatttatttg atacaaataa
    14221 tattatttgt attattttct acacttaa
//
LOCUS       DAELJO010000088        14007 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELJO010000088 DAELJO010000000
VERSION     DAELJO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.088"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            207..1610
                     /locus_tag="KPB88_002216"
     CDS             207..1610
                     /locus_tag="KPB88_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0054490.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2049..3071)
                     /locus_tag="KPB88_002217"
     CDS             complement(2049..3071)
                     /locus_tag="KPB88_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0054491.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3106..4704)
                     /locus_tag="KPB88_002218"
     CDS             complement(3106..4704)
                     /locus_tag="KPB88_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0054492.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVVLKKNHNADDIKKYTAFGMRNHLISQGIDKIDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4746..6011)
                     /locus_tag="KPB88_002219"
     CDS             complement(4746..6011)
                     /locus_tag="KPB88_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0054493.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAVLT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6156..7568)
                     /locus_tag="KPB88_002220"
     CDS             complement(6156..7568)
                     /locus_tag="KPB88_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG0054494.1"
                     /translation="MASTNKNNATSKEEEKEISTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDHIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            complement(7606..8613)
                     /locus_tag="KPB88_002221"
     CDS             complement(7606..8613)
                     /locus_tag="KPB88_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0054495.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8810..10132)
                     /locus_tag="KPB88_002222"
     CDS             complement(8810..10132)
                     /locus_tag="KPB88_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054496.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGKVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10490..12130)
                     /locus_tag="KPB88_002223"
     CDS             complement(10490..12130)
                     /locus_tag="KPB88_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0054497.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12286..12942)
                     /locus_tag="KPB88_002224"
     CDS             complement(12286..12942)
                     /locus_tag="KPB88_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054498.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(12968..13669)
                     /locus_tag="KPB88_002225"
     CDS             complement(12968..13669)
                     /locus_tag="KPB88_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0054499.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4767 a         2633 c         1670 g         4937 t
ORIGIN      
        1 caatacataa tttataattt gtaatacttt attcaataca taagtagaaa tctctatata
       61 cataaattca ttttttcata catcttacaa atttattttt atttagataa catgtatatt
      121 ttttccatga aaaataaata ataaaaaata gtaaatatgg aaaatataaa attgattaac
      181 aataattaat tacctgaggt gtgaatatga aaatgctaaa caactttttt aagaaaatca
      241 acaatccagc tcctgtcttt gcacagacaa aaatggaaag agataatcaa acagatgatt
      301 cactttctac aaaaccaaag actacattta gagacgttgc tggattagac gaagtaaaag
      361 aagaactatt tgaaatagtc gattttatga agtcaccaca aaaatatcaa aaaatgggtg
      421 caaaaatacc taaaggagta ttattttatg gtcctccagg aacaggtaaa accctacttg
      481 catctgctgt agctggtgaa acaaactcat catttttcaa tgttactggt tcagagttcg
      541 ttgaaaaata tgtaggtgtg ggagcaaaaa gagttagaac tctatttgaa aaagctagaa
      601 aagaagctcc aagtataata tttatagacg aaatagatgc tgtaggagca aagagacatc
      661 ttgaaagtaa caacgaaaaa gaccaaacac taaaccaact tttagttgaa atggatggat
      721 ttaataaaga ttccaatgta ttaataatag gtgctaccaa tagacttgac ttattagatg
      781 aagctttact tagaccagga agatttgaca gacacattca tataggtgca ccaaactatc
      841 acactagatt tgaaattcta aaggtacata cagatgacaa acctatagac aaatctgtaa
      901 atctagaact tttagctaaa aaaactcatg gtttcaatgg ggctcatctt tcaaatatag
      961 caaatgaagc agctatattt gcagttagag atgacagtga gtgtattaca tcagaacatt
     1021 ttgacaaggc acttgaaaga gttattgcag ggctagaatc taaaaactct gcattggttg
     1081 aaaaagagaa aaaaatagta gcatatcatg aagctggaca tgctcttgta agtgatatag
     1141 taggaatttg tcctatacaa aaaatatcta tagttcctag aggacaagca ttaggttatg
     1201 ttcttcaatt acctgatgaa gatagatata tttatactaa ggatgagttg attggaaaaa
     1261 taaaaatatt acttgctgga aaggcttcag aagaattaat atttaatcat aaatctactg
     1321 gtgcaaagga cgacttaaag aaagtcactg aaatagctaa ccaaatggtt tgtgaatatg
     1381 gtatgagtaa cttaggattt atgactattg atggaaatga taagactttc ttatgtgata
     1441 aggttcaaaa ggaagcaaat cgtatagttg aaatttgtta taaagagact ttagagatgt
     1501 taaaggataa tcttgaagat ttacattctg tttctaaatt cttatttgaa aaagaaacta
     1561 tgactcatga agagttaaaa gatttgatag gaaaagaagc tgtaaactaa tatagtttta
     1621 aaaatttgag gctgtctcaa aatagagata agcctctttt tattgttaaa gttataaaaa
     1681 tacatttgat tttatacaaa aaagtgcctc ataaactaaa aatttcattt tgagacactt
     1741 acatatttta attacatatt ttaattttgt ctatttacag aatattatat ctacataaaa
     1801 ctaaactgat gtaaacttaa ctacctccag taatgtttga agtggtataa aagctagtag
     1861 cctagtttct acacttataa tttatatttt acataactaa atttagtaga tttttatact
     1921 cacaatttag aacgattgaa aacttaaaaa tttcttatca gatatctaca acttaattag
     1981 gtatctacca cttaattaaa tatctacaac ttaattaaat atctaccact taattaaata
     2041 tctaccactt aaagtgtaga acctctttca atcaattcag gttttaacat ttcaacaact
     2101 gactctggat tttcttcatt taataaggac ttaagtatgt taattgtttt ttcaatcaac
     2161 aaatctagtg gctgtctaat cgtcgttaaa ttataactct tccatgaagc catttgaata
     2221 tcatcatatc ctatcactga taaatcctct ggcaccctca acttgtactc aaaccttgca
     2281 gcatccatta caccaatagc tatcaagtca cttgtgcaaa aaattgctgt tggaggattt
     2341 gataatgcta acattctctt acctgcttca tatccagaat cataatcata gtttgcagat
     2401 tcttctttaa ggttatgaat cccactttgt cttaacttag aataaaatcc aattttcttt
     2461 tccatttctt cacttgtttc tctcgtaaat tgaatatatc ctatattctt atgacctttc
     2521 ttaagcagat attcagcagc cattccccca ccttcaattg ggtctacata gactgtactt
     2581 atttttatac caggtataaa acgattaaaa agaatcacag gaatattgtt ttcttcacaa
     2641 gtctcagcca tcaccttatt catggccgaa gctgtaataa tcacagcttc tacttgaaac
     2701 tgaattactt ttgaaattat actatctata ctttgttgtc taggaacttt aaaaacgaga
     2761 cactgtttac ctatctcttg aattttttct acaaataaat tgataattct attgtaaaat
     2821 ggacctagac tatcaccaac tatcaatcct ataacattag tttttccact aatcattccc
     2881 cgtgctacca aattaggaac atatcctatt tcgtttgcta cacgaaaaat tctttctctt
     2941 acttcaggtt ttacgccctt tccatttgca ctaccaaatg ctcttgaaac agatgattga
     3001 gatactcctg ccaatcttgc aacatctttt gaggtagccg atttattata atgaactttc
     3061 ttttgattca tttagtattt ctccttaaat atctagtatt tcttcttaaa attgtatcaa
     3121 aaataatata atatattgga taataaaaaa agctaataca attagttctt gtttacctaa
     3181 agaaaatggc actatagata ctccatctat tttatcaatg ccttgagaaa taaggtgatt
     3241 tctcatacca aatgcagtgt attttttaat atcatctgca ttatgatttt tctttaaaac
     3301 gacactaaaa atatatccaa atatcaatgt tattgtagca cctattgctg gattccacaa
     3361 ccaactcaca ttatatttat tagaaataaa aaatcctaaa actaacccta caataaaacc
     3421 aatagatact cctttatcat tagctttata tgtaaacatt gctagtacaa atgcacctgt
     3481 agctggtcca gcaaagtatg agatatagtt accaacaagg tcaagaattg atttgatgct
     3541 tcctccaaat cccataataa taaaacctgt cataacaact cctataacta cagttataat
     3601 cattgtaact tttaatgatg attctttgtt atcttttttg aaataaaact cataaatatc
     3661 cttcgtaaaa acagttgtca tactgtttaa tatagagtcg atactagaca ttgctgctgc
     3721 aaacacacct gcaataataa ttccaatggc tcctactgga agttcattta aaataaaagt
     3781 aatcattatt tcatttgaag tttcaaatgt ccttccccca taaaaaacaa ataaaattac
     3841 tcctaccaat agcataaagt agtaaactaa attcataaca aatgcactaa ctacaaaaga
     3901 attttttgca cattttaaag atttagatgt taaaactctt tgtacttgag attggtcaaa
     3961 acagaagtat cttatccaca ttacagttcc accaattaaa gtagcccaaa aagtatttgt
     4021 aactgaaata tccctactga aatcaaatgt atttaatttt ccactctgtt gtgcaatttg
     4081 taatgtatca aagaaactta gattcattcc ttttaaggct acaataatta ctataaatac
     4141 agcacttatt actactatca tttgaatgct atcagtccat ataactgcct taatcccacc
     4201 taatagagta tatataattg atacaagtac aactattggg actaaagcat aaagtgacca
     4261 gccagtaatc atttgaataa taagcactgg aatatatacc atagaactag cttgaatcaa
     4321 tgaattaata aaaaattgaa taatagttaa tcccctcgaa taatttccta aacgaagtcc
     4381 catatattca tatatagagg ttatttttaa tttatagata actggggtgg taatgctcag
     4441 tgcaaaaaat atcgctaaag gtactgtaat attaaccatt actggataca ttccatctac
     4501 atatgcccat ccaggaccac ctataaagcc atttgcacta atcatagtag ctgcaactga
     4561 taaagcaatt ggaatccatg gcattgacct acctgccaag aaataatctt cttgattttt
     4621 attatctttt cctgaataat aaccaataac taacattaat agcaaatatc ctattataat
     4681 acatacatca attgatgaaa tcataagcca cccctttcta agtaacaaaa taaattccta
     4741 gtattttatt tcttattatt taacgtctct ggtaagaata accacacaat agcacctgct
     4801 acaaagactg ctattgatat gtaaaagata gtcatcatac ctaaagtacc agaagctgta
     4861 ccagctatag caggtattgc aaatgtagat ataatccttc ctccattaaa tgacatacca
     4921 gatgacaacg aacgatattt acttgggaac aactctgtgt agtatgctcc ccatgctcca
     4981 gaatatccaa cagcaacacc tagtaatact gatatcatat agaatgttgt tgtattatct
     5041 ccacctaatt ttgtatatat aaaaacaaag actgctgcta atgccatacc tataaaaggt
     5101 atttttcttc caaatttatc tgcaattgaa ccccagaata aatatcctac taattgacca
     5161 attccctgta aactgtatat agaaccagcc actgctgctg acattcctat tcctgtcact
     5221 aggtatgtag ttgcattatt tgaaaatgct gaatatgctg taaaatttgc acctgataaa
     5281 agaatagttc ccaatccaat ccataagtat tttcccttat acatacttat atagctaatc
     5341 ttttcaccag tttcttttcc ctttttaact aattcctttg tatactccaa atcatctgga
     5401 acaaagaaaa ttgctaagat agctccaact ataggtggta taatcgcagt atacatcagt
     5461 atttgccaat ttgcatcacc taaaacgcca tatactttcc caccaaaaat catggctaat
     5521 gaaaataaag cagttaatac agcagctaac ttaccacgat atttacttgg aaacatatct
     5581 gcaactaaag gcatagctgt tccaaataca ccccctaaca ctactcctgt acacaaacgt
     5641 gctattttcc ataacataat atttgtagga gataacattg gtataaatgt tgtcaatcca
     5701 atcagtaagg caacactaat taaagtcctc ttcttcccaa tttttgtaga aatcacccca
     5761 aagataaacg tacctggaat tgtaccaaaa gacataactg caaatatttc ttctgtttgt
     5821 tttaatgaaa aaccaaatcc cccatctgct aaagcataaa tcattgttgg tttaataaaa
     5881 gtacccacca taaaattgta gcaataaaaa aagtatatta aagcaattga aaaaaatgca
     5941 attcctcttt ttaggtttga atagtctttt cctaatatga tatcattttc tatttcaacc
     6001 gttgttttca tatttttata attccccctt ttaaatatta gtcataattt tgctaaaagt
     6061 ttaaacatct atttttcata tacattcccc acttaaattt tacaaagcaa tacttccata
     6121 tgtacatttc aaaactattt atactctaaa cagccttatt cttctactcc catatacatt
     6181 tgttttaaga catccatacc atcaaaagtt atccactctt cctttacttt accttgtagt
     6241 atatgataat gattgattcc cataacctct atatgctttc cagaaggctg tccaaaaaat
     6301 cctatacctt catttattcc acgtaatctc catcttacag atacatcata accttcattt
     6361 ttttctcttt gattacaagt tactctttcc acttcataac ttccatttgg aattgatgca
     6421 agtaagctta caatcattcc ttgaatctca tcataaccat ttaaatcttt atcacatata
     6481 aaatggacta ctgcattatc atgatagtat tttttaactt catttatata tttataatta
     6541 taaatacgac ttgtcatttc aagcatcatt tcgccaacag aatcatcttt tgcttgatac
     6601 ttagttggca taaattgacc atccatagat tcacagatac cataagttga ttgtaaacta
     6661 agcactttag attcacttgc ctttgccata ctctttgcta cttcctgagg gtttaaccct
     6721 aattgagtaa caatccataa attatctcta accaaccatt cttcatggat tctattgtta
     6781 gttgtggcac aatcaattac agttctaaag tttatttttc tacctgttgc tggtccaaaa
     6841 ttactatcac ctaagttagt tgctgtagac aaaacacggt gagaagataa aaaaccatca
     6901 gcaccaaagt tagaccatat tacattttga ccaattaatc ttctatcagg aaacgcatgt
     6961 aatgtttgaa gagtattgga aataacatct ttaataccaa ctaaatttga agagccacaa
     7021 tgcattgtaa cattattgtg atatgtatca taaataacac ctataccttt ttcttcccag
     7081 atattatgtg taatcttcat aatatgatct acaaaatctc tatacttaga atcaaatcca
     7141 ggtatttcct gttccctttt tgtctgtttt gaaaaatcat tgtaatccat tactccaaca
     7201 ggcgttactt ctaatgaatc tccatagtaa atttttgtag atgacatatt atcttccccc
     7261 ttaaatataa cttttttatt tttttcagta agagtatttg tatttaattt atctgtatcc
     7321 actgtatcta tgttaacttt atttatgtcg gatttatttg tgtttgtttt atctatattt
     7381 cctttatcta tatttccttt atatgtgttc gttttattct catttgtttg attttcattt
     7441 atattactaa aattagtttg attcatttta gctttttttt ctttaaccgt acatgattca
     7501 agcacacttg tagaaatttc tttttcttcc tctttacttg ttgcattatt tttgttggta
     7561 gatgccactt tatatccccc tttgttttag aatacgtatg cataactaag cattaaaaat
     7621 acttttagtt ctctcaagaa tatcaagccc ttgttgtttc aaccaataag gaatatcaat
     7681 taacacccag ttttcttgaa gtttatcgcc cttacgataa taaacatcaa caacttgcat
     7741 atctgcttta acctctccac ctggtaatcc taaaaaacct ccacatggta cattagtcag
     7801 attaggccat ccaaagaaac aagcaaaatt accttctgca aatctacata catgcccatt
     7861 gaaagtcttt ccttttaaat tatttctaaa tggtagctgg tgttgctctt gatatctagg
     7921 tatagtataa gaagcaccaa ttccacctgg accataccaa atcatatctt tagcccaaga
     7981 tttttcaagt atttctggtg ggcatcccat agcaccactt tcatttaatt cagataaatc
     8041 atctaccatt ttatttacta atgctaaagt ttttactcct tcaacttcat ctgcatcttc
     8101 aaaaagtaat ccattatggt caattggccc tggatataca aaatatgaac ctgtttgtgg
     8161 tggcaatgga ttaactcctg cttgaatcat aaacccaatt atatctacaa ataatccagt
     8221 tttagcaatt ttaccatctt gaacacagtt aaattctgca tatcttaaac taatcatttt
     8281 acgagtatgt ttaatgccaa gccaatcgtt atcaaaaagt cccataaaat gccccatact
     8341 cattacccat tcactattgt caatctcatt tgttcctgca atgaaaatat cttgtcgtct
     8401 ttgcatatta ctcaatgatt tttttaaagg cttccaaaat acatctgcta cattttcagt
     8461 gccttcttgt tcacgaaatg gatacacacc tttccagtta taatctgatt tcatatgttg
     8521 ctttaagaca ttttcaacct catccccagt agcattttct aatgcttcaa aataatttct
     8581 tacaactttt tttgaatcct gatattttcc cataattatc tcctcccaga tttatttaag
     8641 ttataaatta actttctcat ttcatgcata cgatttcctt tttaatgatt tttcagctgt
     8701 tttaagttta ttttatgaat acatatgcat ttttctaaat attaacatat atctaaaaaa
     8761 ttgtaaagaa aatgttatca taaaaattta atttttgact tatatctatt taaaacaaca
     8821 gaaaaggttt aaaacgtaat attctactgg gtctaccata cttgctcata agctgttcac
     8881 tcactacctc acaataacca gaatcacaca atctaagtat aatcctgtcc atagttctaa
     8941 tacttacacc acatatagta gctaattcct ttgatgtaaa ttcagttttt ccactattta
     9001 ttattttatt gaatacttta tatacagtat tagcacttag ccctgtctct gtagcaattt
     9061 gataaaaacg ttcatcaaaa ctatttttct ctttgacagt aggatttggc tttagtggtc
     9121 ccataatttc tccattttca aaaactacat atgctgcatc atttttttga ctttgagcca
     9181 tcttaatact ctcataagcg ttaaatttag cttcatttgc tgtttttcca taaccaattc
     9241 ctatataagt gtttataact gaaacttcac gaatcatatc caacaaataa atatctctgt
     9301 atttctttgt ttctaactca agtatttttt tagttgtaaa aatcataaat tcacttctac
     9361 tctgctctac caaaacacca tctatacggc tattgaaatg gtatagttta tcaagaatct
     9421 taatcctttg tgaaagataa gcgtactcat cttccttcat tagtgaatat tctgaaggca
     9481 tgttcagttt aatagcaaga acaacaatct gattctcatt gtttttcttt gcaatataac
     9541 gcatctgaat gttttttata ctttgcacca ttacgtcctc agaaggcaag gctaaaacta
     9601 ctggaatatt atcttttttc attatatgat aggcctcttc aagacctgta atacaacaac
     9661 atacatcatt attttgataa ttatatttat gaaattcaag cacaaacttt atatagtcct
     9721 cttctaaata cttttgctct gcaaaaaaca tatgttcatc cttaaattca actccaattt
     9781 ctttatacaa tctcttcatc atagccttat cataagtatc tatactaagt ctactaatat
     9841 ctaatttaag caaatatgta atttcaagca acactctgta caatgttgtt tcatgtctag
     9901 gaatataatc agacaataca aaatcttttt gtcttttttc aaaaaattta aatggcgact
     9961 tacctgtaaa aagaactgca tccactttac ctctatattt ttccaataaa tcaggtactt
    10021 caatatatct atcatatcta agaaaatctg cttcaatatt aggaaaattc tttttactta
    10081 ctgctagtat tttatcgatt accatacctg gtcctacata aagtattttc atttcgtctc
    10141 tcctttccta atgatactat tatctcttaa aacattttct aaacctacca tgactaaagc
    10201 cacaggactc ctgattcata gaattttgaa taatcaatta tacctaatat aaaaagttac
    10261 tttaagtttt ttaattacga ctatctgttg aaacattttt gtatttaaat ttttgtattt
    10321 aaatcttatt cattaatatt aattacattt tacaattaat atattgtttt ttctacttaa
    10381 agttaaattc tttagaaaac acaagtagag taatctttat taatttaaca aaaatagcct
    10441 tccttaatat tcaaagaaag actattttta taaaagttct taatgtattc tattttgcta
    10501 tgctaagagg atttttaatt acacgtccag cacatacatc agtttgcttt cctcctgcca
    10561 atgcaatttt accattcacc aacacatatt ccactcccac tgtatactgt ttaggattac
    10621 tataagtcgc tttatcttct attgtatctg ggtcaaatac cacaatgtca gcaaaataat
    10681 tttctttaag tagacccctt tctttataaa tgcctaagcg ttttgctgaa agataagtta
    10741 tctttttaac tgcttcctca aaagtcaaca tacctttttc tctaacatag taagagaaaa
    10801 ttcgtccaaa agtaccaaat gcacgaggat gaggctttcc aagagaaagt aaaccttctg
    10861 tagaaaggtc catactatca gaacctatac atgactttgg atggcaaaca accttatctg
    10921 tatcctcttg accttgtgta tgaacaacta cttgaatttt acttttttct tcaatcaaaa
    10981 gcctaaagaa cgtatcaaat ggattttcac cattgatttt ggctacctcc attaaagttt
    11041 taccctctac atatttattt gcttcagtca ttacagaggc tagtacaaca ccgttccacc
    11101 cacctgctaa ttggtagaaa cattgccatc caggaatccc ttcctccaac tcttttataa
    11161 ttctgtctct attttcttgt acttctaatc gttttaccat attttctaca ccgccttcaa
    11221 aagcccaagg aggtaaaagt gcacccaaag atgtcattcc agctgtatat gggtaacaat
    11281 caaaagttac ttccaaacca tctgcctcag cttcatctag ctttttaaat acctcatcaa
    11341 tagttccaaa attcttatga cccataactt ttaaatgaga aatatgtaga ggaactcctg
    11401 cagtcttagc tatataaatt gcttcatcaa gagcttccat ttcttcatca ccttcatctc
    11461 taatatgaga tatatagtat ccattgtatg gtatcaattc cttacataat tcagccattt
    11521 cttctttctt tgtgtatgct cctggtaaat aaactaatcc tgatgaaaga gctacacatc
    11581 cagcttccat agcttttcta acttccaact tcatattttt aagttgctct aaagtagctg
    11641 gttcctttga aaatcccatt tcattgatac gtacaggtcc ttgtgctaca taagctacaa
    11701 cattgactgc tggagaattt tcagttaagt aatcaaagta ttcttgcatt gtattccaat
    11761 gaagctctaa ttttgttgga attgtaccaa ttatcctagt tccaagataa tcaattaact
    11821 cttgcttctt ctcttctctt atcggagcaa ctcctatacc acagtttgca ataacatctg
    11881 ttgttacacc tgtatggata cggcttcttg tgtatttatc atacacaaga gataaatcag
    11941 aatgagtatg gaaatctata aaaccaggtg atacaaattt tccagttgcg tctatagtct
    12001 ctttagcctc tttttgactt aaatctccaa tagccatgat tttgtctcct acaatcccta
    12061 catcactttt atatcttgaa tttcctgtac catcaacgat tattccattg acaattttaa
    12121 catcaaacat gctttttctc ctttcaagac ttatttatat tcatatttct taataaacaa
    12181 tcctattctt ttttctcaac aaataattct agtctttctc cttaataaac aatcctattc
    12241 tttttctcaa caaataattc tagtctttct ccttaataag caatcctatt cttttgtttt
    12301 attgttatca attacaaaga agacccttgt gataagtatc ccacctaaca caataaacaa
    12361 tgttaataat ccacctggta taccaatttt ttcaccaaag tacataattg ctaacgctac
    12421 agcaatcgta catccaccag ctcgaatatc cttacgtgca atatcaacat aaattgctcc
    12481 aaataatgct ggaagtatat aattaaatga attagtaaca aattcaggta acaaaggaat
    12541 tactttatag cctacaatgg taaaaatggt aatcatagaa gctgatacaa agattgaaca
    12601 acatgtacca attgttccaa caacttcacc ctcatgagtt cctgcttcta cacctgcaat
    12661 tttctgtgcc attgatgctg caggcatacg tatatctcca acagaacctg caagccaacc
    12721 tatataagaa cctggagctc ctagtgtagg ataataagct ataggttgaa caatccaact
    12781 aactccataa gtcgcagcga ctaaccccca aatttgaaat ataacagaaa gcattggcat
    12841 ctgaccaaag cgaataccta catagatagc gggaataaag tttgcaataa ttgcaagacc
    12901 tatagaaatt ctccctattt ttatcatctt tttatcatac ataattatca cctcttatcc
    12961 gttttaatca cgataatata atctgtgtac caaacatacc aattaacatg gcaataccta
    13021 aggataattc ttgtaatcgt tgattctttt taaataaagt tgtcaatact agcataacta
    13081 tagctgaaat aattgctggt gtaagttttg gtatttcaaa tgtttttatt tgatttgaaa
    13141 gaaggtaagc aaataatcca actgtagctc ctgcaagtag ttttttaacc caagtaggat
    13201 tatacttcat gttcatcttt tcaacaattc cacgcattct atgacccaaa ataaatacaa
    13261 caaatagcca acctaagtta tttaaagcca tcccccatag tgcatacgaa aaagcttctg
    13321 ctccaaatgc agcactacct acttctaccc cagataaatt ggatgtaaga gatattactc
    13381 caagctctgt tctagctgct ccgacatcac agagcctcat ccaagtcgta ggagcgccaa
    13441 tcacagctac cagtgataat aatgtaataa caggagatag agaaggtcca atggctgtaa
    13501 cactagctga gcgaattgct gctttaattc ctttcctttc aataccaaga tttgaagcct
    13561 cttttagtgc ctctttcata aatacgattg cttgaacaac tgaaataatt actaaaaaac
    13621 tagaaacaat ccaaagacct gggctattaa tcatttcttg aaacttcata ctttattcct
    13681 ccttaaaata aatttttcag cattttattg tgagctttct ttctgtaatc ttgttatgtt
    13741 tttatattgt tttaatttat tttatctaag atatagtatc tttaatagga gattatctgt
    13801 cttaaagtct cactaaaaac attaatactt aaatatttta ttcataatta gtaaatatca
    13861 gaaaattcac atattatttt tttaacctgt aattaactta taaggtcttt tattattttg
    13921 acatttcaat ataaaaattt taatcaaaaa actttattat tttaaaaaaa attattattt
    13981 ttataattta tattttttaa gtttatt
//
LOCUS       DAELJO010000089        13887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELJO010000089 DAELJO010000000
VERSION     DAELJO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.089"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            295..1134
                     /locus_tag="KPB88_002226"
     CDS             295..1134
                     /locus_tag="KPB88_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG0054500.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1131..2312
                     /locus_tag="KPB88_002227"
     CDS             1131..2312
                     /locus_tag="KPB88_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054501.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2616..3518
                     /gene="ptb"
                     /locus_tag="KPB88_002228"
     CDS             2616..3518
                     /gene="ptb"
                     /locus_tag="KPB88_002228"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG0054502.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3540..4619
                     /gene="buk"
                     /locus_tag="KPB88_002229"
     CDS             3540..4619
                     /gene="buk"
                     /locus_tag="KPB88_002229"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0054503.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4609..5286
                     /locus_tag="KPB88_002230"
     CDS             4609..5286
                     /locus_tag="KPB88_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0054504.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5329..5544
                     /locus_tag="KPB88_002231"
     CDS             5329..5544
                     /locus_tag="KPB88_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0054505.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5573..6652
                     /locus_tag="KPB88_002232"
     CDS             5573..6652
                     /locus_tag="KPB88_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0054506.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6652..7404
                     /locus_tag="KPB88_002233"
     CDS             6652..7404
                     /locus_tag="KPB88_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0054507.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7405..7962
                     /locus_tag="KPB88_002234"
     CDS             7405..7962
                     /locus_tag="KPB88_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0054508.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8132..9478
                     /locus_tag="KPB88_002235"
     CDS             8132..9478
                     /locus_tag="KPB88_002235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG0054509.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9745..11577
                     /gene="glmS"
                     /locus_tag="KPB88_002236"
     CDS             9745..11577
                     /gene="glmS"
                     /locus_tag="KPB88_002236"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG0054510.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12028..13323
                     /locus_tag="KPB88_002237"
     CDS             12028..13323
                     /locus_tag="KPB88_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG0054511.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         5520 a         1538 c         2730 g         4099 t
ORIGIN      
        1 tattaaacaa agatatatta agatatttta gaacacaaaa ctgcattaag gcgcgagtac
       61 tattttgtat tcgcgccttg atggtttgag ttatttgagt aaaaactaac tttaataaga
      121 aatctgtatt tattaagttg atatttttaa tgtgtttaga gattatttaa caatctccaa
      181 atataaaact agaattattt acataaaagt taaatagttt gtatagagtt agatttatat
      241 agtataatac ttagtgtaaa taattaagaa gttatcaaac aggaggataa aactgtgtta
      301 gatataaatt cttttttaaa tggatttttt aatataatat taagaatgag tatatatgat
      361 ttaattgaca tatctatagt agcgtatatt ttttacaaaa tatttatgtt tataaaagat
      421 acaagagcag aacaagtgtt taaaggtata atatttcttt tattagcaac tcaacttagt
      481 aacaccttta aattgcatac cgtttattgg atatctctta aagctttaga ttatggagtt
      541 atagctgctc ttataatttt tcaaccagaa tttagagcag gacttgaaca tataggaaga
      601 gcaaagttta atttatttgg aaagaatgta aatacttctg aagaaactct caatagaaat
      661 atagaagaaa ttgtagaagc tttatattca ctttctagac aaaaaatagg agctcttatc
      721 ataatggaaa gagaaactag aataagtgat ataataaata caggaactat tattgatgct
      781 gaaatttcta ggcaactatt aataaatata tttataccaa atacaccatt acacgatgga
      841 gctgttgtaa ttagagattc taaggtaaaa gcagctgcat gttttttgcc attgacagaa
      901 agtaaggatt taagtaaaga tttgggtact agacatagag caggaatagg ggtaagtgag
      961 gtgtctgact gtataacttt gattgtatca gaggaaacag gtggcgtatc aattgctaaa
     1021 gcaggtaagt tatatagaga tatttctaga gaaagaatga tgaatatatt acgtagtaat
     1081 ttaaaaacaa atacagaaac aagaagtttt ttcaaaggtg gtatatttaa atgataaata
     1141 gactaaaaaa taataccaaa ataaaaataa tatcattttt aagtgcaatt gcattatgga
     1201 tgtatgttat ggctgtagtt gaccctgaag aaactaaagg gtttgaagat atacctgtat
     1261 ctataagtaa tatgagtgat ttaaaggata aaaatcttgt tatatatcca gacgaaaaat
     1321 taacagcaga tgtatatata aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag
     1381 atatacatgt atatggaacc attgaagacc caatacaagg acagaaggaa gtatatttaa
     1441 aagcaaatat tcctcaaggt gttactctag aatttaagca tgatgtttta gtagtaaatc
     1501 ttgaaaaaaa cataaaagaa aaaagagata taaaggtaga agtaagtgga aattcaaaaa
     1561 gaaatataaa taaaattgaa cttagtaagg aatttgtcga tgtatcaggt ccaagaactc
     1621 tagttaaaga agtcaaaagt gtaagagcta atctagatgt tggtaatgag acagatgatt
     1681 tttctaaaaa attgcaatta ataccattaa atagtgatgg aactaaggtt gaaggagtaa
     1741 cattaagttc atcctttgtg actgcaaaga tagaactatt gaaggaaaaa attgtcccaa
     1801 taagagtaag tctaaatgat aataaagata atgatgtact gaaaaactat aaattaagta
     1861 aggaacaagt tacaataaaa ggaaagaaag aagatataga caaaataagt tatattaaga
     1921 caaaacctat tgacttagct gatttggcta atggtgaaga aaaagaagtt agcttagaaa
     1981 ttccagaggg tataactctt gaggatagct ctataacaat taaattaaat gatacaaaac
     2041 aattgagtgc agaattttta tataatgcgg gagatattga attaagaaat atcccaacaa
     2101 atatggataa tccaaatgta atttctgatg tagatataaa agttgttgta gagtcaacgg
     2161 aggatttgag taagttaaat aaaaaagata tttcattata tattgattta tcacaaggcc
     2221 ctgaggaaga tagtaaatat aaaataaaat atgaaacaac atatcaattt aaaaaaataa
     2281 atatagaacc taatattgtt gaagtggaat gaacaaatat ttcgaatatt tagttgttac
     2341 aagatgcgac aaaaaaacct ataaaattaa gtttgttaat tttatgtttt tgttgcatct
     2401 tgtttatttt agttatatta taaaataagg acaatgagat atttgttaaa tctaaaaagc
     2461 gctagatata tgaaaaaaga ctgataaata gatttaaata aatattttga attaaatatt
     2521 tcaattatct ctaggaaaac gttgctgaac gagttataat ttaattgaga tataaaaagt
     2581 tctatttcaa aaaattcaag atgaggtgga aatgcatgag aagttttgaa gaagtaatta
     2641 agtttgcaaa agaaagagga cctaaaacta tatcagtagc atgttgccaa gataaagaag
     2701 ttttaatggc agttgaaatg gctagaaaag aaaaaatagc aaatgccatt ttagtaggag
     2761 atatagaaaa gactaaagaa attgcaaaaa gcatagacat ggatatcgaa aattatgaac
     2821 tgatagatat aaaagattta gcagaagcat ctctaaaatc tgttgaatta gtttcacaag
     2881 gaaaagccga catggtaatg aaaggcttag tagacacatc aataatacta aaagcagttt
     2941 taaataaaga agtaggtctt agaactggaa atgtattaag tcacgtagca gtatttgatg
     3001 tagagggata tgatagatta tttttcgtaa ctgacgcagc tatgaactta gctcctgata
     3061 caaatactaa aaagcaaatc atagaaaatg cttgcacagt agcacattca ttagatataa
     3121 gtgaaccaaa agttgctgca atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata
     3181 cagttgaagc taaagaacta gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg
     3241 ttggtggacc ttttgcaatt gataatgcag tatctttaga agcagctaaa cataaaggta
     3301 taaatcatcc tgtagcagga agagctgata tattattagc cccagatatt gaaggtggta
     3361 acatattata taaagctttg gtattcttct caaaatcaaa aaatgcagga gttatagttg
     3421 gggctaaagc accaataata ttaacttcta gagcagacag tgaagaaact aaactaaact
     3481 caatagcttt aggtgtttta atggcagcaa aggcataatt ttaaggggga aataggcaaa
     3541 tgagcaaaat atttaaaatc ttaacaataa atcctggttc gacatcaact aaaatagctg
     3601 tatttgataa tgaggattta gtatttgaaa aaactttaag acattcttca gaagaaatag
     3661 gaaaatatga gaaggtgtct gaccaatttg aatttcgtaa acaagtaata gaagaagctc
     3721 taaaagaagg tggagtaaaa acatctgaat tagatgctgt agtaggtaga ggaggacttc
     3781 ttaaacctat aaaaggtggt acttattcag taagtgctgc tatgattgaa gatttaaaag
     3841 tgggagtttt aggagaacac gcttcaaacc taggtggaat aatagcaaaa caaataggtg
     3901 aagaagtaaa tgttccttca tacatagtag accctgttgt tgtagatgaa ttagaagatg
     3961 ttgctagaat ttctggtatg cctgaaataa gtagagcaag tgtagtacat gctttaaatc
     4021 aaaaggcaat agcaagaaga tatgctagag aaataaacaa gaaatatgaa gatataaatc
     4081 ttatagttgc acacatgggt ggaggagttt ctgttggagc tcataaaaat ggtaaaatag
     4141 tagatgttgc aaacgcatta gatggagaag gacctttctc tccagaaaga agtggtggac
     4201 taccagtagg tgcattagta aaaatgtgct ttagtggaaa atatactcaa gatgaaatta
     4261 aaaagaaaat aaaaggtaat ggcggattag ttgcatactt aaacactaat gatgctagag
     4321 aagttgaaga aagaattgaa gctggtgatg aaaaagctaa attagtatat gaagctatgg
     4381 catatcaaat ctctaaagaa ataggagcta gtgctgcagt tcttaaggga gatgtaaaag
     4441 caatattatt aactggtgga atcgcatatt caaaaatgtt tacagaaatg attgcagata
     4501 gagttaaatt tatagcagat gtaaaagttt atccaggtga agatgaaatg attgcattag
     4561 ctcaaggtgg acttagagtt ttaactggtg aagaagaggc tcaagtttat gataactaat
     4621 gataaaagaa taagaatcat aactggtcac tatggaagtg gtaaaagtga atttgcgatg
     4681 aactatgttg ttaagttaag agatatggtt tctggcaaag ttgctatagc cgatttagat
     4741 gtggtaaatg tatactttag aactagagaa aagaaagagt taatgaaatc cttaggtata
     4801 caaccaatag atagctcaat taatgctcca acattagatt taccagcagt atcagcagag
     4861 gtaatgtctc ctatggtaga ccattcatac aattctgtta tagatttagg tggagataat
     4921 gtaggtgcta gggttatagg aagatttagt catttgttaa aagaaggcga ttatgatatg
     4981 ctttttgtaa taaatgcaaa tagggagaaa actcaaactt ctgaagaagt tatccaatat
     5041 ataaaagaaa tagaaaaatc ttctaaatta aaggtaacag gtctaattaa taatactcat
     5101 cttattaggt ttactactat tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt
     5161 tctgaaaaat gtaatatacc gataagatat gttgcatgtt tagaaaatct tgttgaacag
     5221 ttaccaaaag atttagaagg agaaattttt cctataaaat tatatatgag agaagactgg
     5281 atgtagtttg agctcaaata tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga
     5341 aaagtatctt ttaatcaaga acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca
     5401 gtaaaaatca tacaattaga ctcaaatgta ataaacaaaa aagggtataa cccagcaact
     5461 gtttttgaaa tggaaaaatg cataggatgt gcaagttgtg caactatgtg tccagatgta
     5521 gttataacag tagaaagaga ttaatttaat taatatttaa gggggaattt taatggctaa
     5581 gatacttatg aaaggtaacg aagcatttgg aaaagcagct atagaagctg gttgcaaata
     5641 tttcttcggt tacccaataa ctccacaaag tgaattacca gaatatttat caagagagtt
     5701 acctaaaata ggtggagcgt tcgttcaagc tgaatcagaa gtttctgcga taaacatggt
     5761 ttatggtgga gcaggtgcag gagcaagagt tatgacttct tcatcttcac caggagttgc
     5821 tttaaagcaa gaaggtataa catatgcagt aggagcagaa gttccttgtg ttgtacttaa
     5881 tgtaatgaga ggtggtccag gacttggaag tatacaacct tcacaagctg actactttat
     5941 gtctacaaga ggtggaggaa atggagacta cagaactcca gtattcgcac cagctacagt
     6001 tcaagaagct gttgatatga taatggaagc ttttgacgtt gcagattatt acagatcacc
     6061 agttatggta gttgcagatg gtatgatagg tcaaatgatg gagccagttg aatttagagc
     6121 tcctgagaag aaaagagaat tgcctcctaa agattgggca actgttggaa ctaaaggaaa
     6181 aagaaaacct aatgtaataa actctctata cttagagcca gaagtactag aagaccattg
     6241 ttggcattta caagaaaaat ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta
     6301 caaaacagaa gatgctgaat ttgtatttgc agcttatgga actacttcaa gagtagtaaa
     6361 aagcgcaatt gatatattaa gagaagaagg aataaaagca ggtcttataa gacctaaagt
     6421 attatggcca ttcccattcg aagcattcaa ccaaattcct aatgctagaa acatattgac
     6481 tgttgaaatg agtatgggac aaatggtgga agatgttaaa atggcagtag aaggtaaatt
     6541 accagtttac ttccatggaa gaccaggagg aatgactcca acaccagcag aaatagttga
     6601 aaaagctaaa aaaattattg ccggagaatt ggttgcagga ggtgctagat aatggctgtt
     6661 gtatttaaga aaactgaagg attacaagat actcaaactc actactgtcc aggatgtaca
     6721 catggtataa tccatagatt agttggtgaa gtattagaag aactaggagt attaggtgat
     6781 gctgtaggtg ttgttccagt tggatgttca gttttaggat ataaatattt taactgtgac
     6841 actcaagaag ctgctcatgg tagagcgcca gctgctgcta caggaataaa aagagttcat
     6901 cctgaaaata cagtattcac ttatcaaggt gatggagact tagcttcaat tggtactgct
     6961 gaaatagttc atgcagctgc tagaggagaa aaaataacta ctatatttgt aaacaataca
     7021 acttatggta tgactggtgg acaaatggct ccaactacat tagttggaca aagagctact
     7081 actgctcaat caggaagaaa tgctgaaact caaggttatc caataagagt aagtgaaatg
     7141 ttagctacat taacaggagc tgtatttgta gaaagagtag cagttgatac tccagctcat
     7201 gttagacaag ctaaaaaagc tataaagaaa gcattccaag ttcaacaagc aggattagga
     7261 tttggaatag tagaagtatt atctacttgt cctacaaact ggggactagc tccaaatgat
     7321 gctttacaat ggttaagaga caacatgata ccatactatc cattaggaaa tttcaaaaat
     7381 gttgaagttg aggaggtgaa gtaaatgtct acagctagag taatatgtgc aggcttcgga
     7441 ggccaaggtg ttatgtctat gggacaatta cttacttatg caggaatgct agaaggaaaa
     7501 gaagtttctt ggttaccatc ttatggacca gaaatgcgtg gaggtacagc aaactgtgca
     7561 gtaactgtat ctaatgagcc agttggttct ccacttataa cagatgatgc aactgcagct
     7621 atattattaa atataccagc atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa
     7681 ataatagtaa atagttcttt aataaaagaa aaagttgata gaacagatgt agatgtttat
     7741 tatataccag caaatgaatt agcagctgaa ttaggaaatg ataaagttgc taacatgata
     7801 atgttaggcg cttacttaaa agtatctgat actgtagata tagaatctgt actagaagca
     7861 ttcaaaaaag ttttcggacc tagaaaagaa aaattcgttc cattaaacag agaagcttta
     7921 caaaaaggta tggatgctac atgttctaaa gcaactaact aataaagaaa aagatgtcaa
     7981 aaaaaagaca atagaattat ttctattgtc ttttttgctg ataagtatta aaatattttt
     8041 atgactgaaa ttaagcctat aatgatattt tatgaactat aaaaatataa tacaaattac
     8101 aagcttttca attatgaaag gagaatcttt agtgagaaag tattttggaa cagatggagt
     8161 tagaggagta gcaaatacag aactaacatg tgatttagca tataaacttg gtagagctgg
     8221 tggatttgtc ttagctcaag gagaccacag agtaaaagta gtagtaggta aggatactag
     8281 aatatcaggt gatatgttgg aagcatcttt aatagcagga cttatgtctg ttggatgtga
     8341 tgttattact gttggaataa taccaacacc tgcagtggca tatttaacta gaaaatatgg
     8401 tgctgattgt ggtgtggtta tatctgcatc tcacaatcct gttgaatata atggaatcaa
     8461 attctttaat aagaatggat ataaattaga tgatgaaatt gaattgaaaa tagaagaata
     8521 tatagacgat atagacaaaa ttgattgtct tccaattggt gaaaatgtag gaagaaaatt
     8581 acatgaacat tgtgcacaaa gagactatgt agactaccta aaatcaataa taagcactga
     8641 ctttaaggga ttaaaagtag ttttggactg tgctaatggt gcgtcatata aagtggcacc
     8701 aatagtgttt gatgaactag gagcaagtgt aatttcaata aatagttcac cagatggaaa
     8761 taatataaac tataaatgtg gctctacaca tccagaacaa cttcaaaggg ctgttttgga
     8821 acataatgct gacttaggtc ttgcatatga tggagatgct gatagattaa ttgcagtaaa
     8881 tgaaaagggt caaattgttg atggagacca tataatgata ttgagtgcct taaatttaaa
     8941 gaaaaataat aaattggcac aagatacttt ggtagttaca gttatgagta atatagggtt
     9001 gacaatagct gctaaggaaa atggaataaa tttatctact acagcagttg gagacagata
     9061 tgttttagaa gatatggtta aaaatggata taatcttggt ggagagcaat ctggacatat
     9121 gatattttta gattacaaca ctactggaga tggagtgctt agttctctaa tacttgcaaa
     9181 tataatcttg caagaaaaga aacctttatc agaaattgca tcaataatga gccaataccc
     9241 acaagtatta gtaaatgcaa caataaaaaa tgaaaataag aacaaatata tggaatatcc
     9301 agaaataaaa actgagatag aaagaataga atctatttta gacggaaatg gtagagtttt
     9361 gataagacca tcaggaactg agccattagt tagagttatg ttagagggta aagaggaagg
     9421 tcaaataaaa gaattagcaa caaacttagc taatcttata caagaaaaat tatcgtaatt
     9481 taaagggtat tctcagatat aaatactgag gacacctttc taagcgccag aacttagtta
     9541 tagacataat tattgagtaa tataggaact aagttgacga ggtcagagtt aatcgaattg
     9601 tcggcggatg ctctgcggtg tgtttaccat cgaaagactt ctttaaataa taggagcaat
     9661 cctatggaca aagggaagcc aaagtaaact tatatgacct aactcttaaa atatataaat
     9721 aatattttta ggaggacaca aataatgtgt ggaatagttg gatatttagg aagtagaaag
     9781 gcagcagaag ttatagtaga agggctttct aagctagagt acagaggtta tgactcagca
     9841 ggagtagcag ttaatagttc taatgaaaaa gaacttaata ttagaaagtt taaaggtaga
     9901 ctttctgtac ttgcagaaga cttagaaaag aatcctattg atggaaattt aggtataggg
     9961 catacgagat gggcaactca tggagaacca tctgatgtaa actcacatcc tcattttaat
    10021 caagccaaaa caatagcagt tgttcataat gggataatag aaaactatat ggaaataaaa
    10081 gaagaattaa tttctgaagg tgttaagttt gaatctcaaa cagatacaga ggtaatagca
    10141 catttagtag ataaatacta tgagggtaat ttattagatg ctgtatacaa aactatatca
    10201 aaattaagag gagcttatgc attaggtgtt atatgtaaag aacatggaaa tgaattagtt
    10261 gcagtaagaa aagatagtcc tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca
    10321 tcagatatac ctgcattatt aaaatatact agagatgttt atttcttaga aaatggagaa
    10381 gtagttcatc taaaagacga aaatgtaacc gtttatgact ctaatagaaa tcttgttgaa
    10441 aaagaagtat ttcatgtaac ttgggatgta gaagctgcgt ctaaaggtgg atatgactac
    10501 tttatgtcaa aagaaattca tgagcaacca acaggagtaa gagaaacttt agaaagaaga
    10561 cttgatgata atggaaatat aattctagac agtataaata tttctaaaga agacttagaa
    10621 aaaataaata aagtttatat agtggcttgt ggaacagcat ataatgctgg attacttggt
    10681 aagtatgcca tagaaaaatt tgtaaatata ccagtaataa ctgatatagc atcagaattt
    10741 agatatagcg acccatttgt agatgaaaat tcattagtaa tattagttag tcaatctggt
    10801 gagacagcag atactctagc agtattaaga gattcaaagg ctaaaggagc aagaatactt
    10861 tcaataacta acgttgtagg ttcatctata gcaagagaat cagatgatgt attctatact
    10921 tgggcaggac ctgaagtagc agttgcatca actaaggcat atactactca aataacttct
    10981 ttatacatga tagctttaga ctttgctatc aaaaaaggaa caataactag agaattttat
    11041 gatagtatga tttctaagat gaaagaaata ccatcaaaaa tacaagaaat actagacaat
    11101 gaagaatata taaaagaagt tgctaaaaca gttgttagtt ctgaacatgc attctattta
    11161 ggaagaggta tagactatag cttagcaatg gaaggttcat tgaaattaaa agagatttca
    11221 tatatacatg cagaagcttt tgcagcagga gagctaaagc atggaacaat agctttaata
    11281 gaaaaaggaa ctcctgtaat tgccatagct actcaagaaa agttatttga aaaaatggtt
    11341 tctaatatgg aagaagtaag agcaagagga gcttatgtag tagcaatagc tcaaagccat
    11401 aataaggatg ttgagaaagc tgctgataaa ataatctata taccaaattc agatgatatt
    11461 ttatcaccaa tattagcagt tgtaccaatg caattacttg cttatcatgt ctctgtacta
    11521 agaggatgcg atgtagataa gccaagaaat ctagctaaat cagtaacagt tgaataaaaa
    11581 cagattaaaa attaaataga taatatttat tgaaaagaac tcatcctgtt atttgtattt
    11641 tataggatga gttttttaga taaatattaa atttgttttg tttttgctat aataaatttt
    11701 ataaaaagat tgatattaaa tcatctgtaa aatgataaat atatagacca taccaggtat
    11761 agggaacgat aaatatagtt catataaaat atagtgagta cttaataaac atatagtcaa
    11821 tatcagataa aaaaataata gcttatgaga aaaattaaaa tacaaggtat tagtaacctt
    11881 gtgataagat aaagtgagta gtttaaaata agaatataag gaactattta taaaagaata
    11941 taatgaagct aatagagtat aagaaaatta ctttatttta gtaaaataat ttcaaataaa
    12001 aattttttta aattgggagt ataaattatg acaaatgatt ttaaaaaaga aaagaaagaa
    12061 gattattttg ttaatcttaa ggcaatatct actgaagttt tacaagaaaa agtgcaagat
    12121 aacgcttggg ttatagttga tactcgctta aatgatgctt ataatggctg gaagttagat
    12181 ggagtaaaaa gaggtggaca tataaaagga gcagtagatt tttctgctaa ttggttaagt
    12241 gtatactcag atagaaaaga tgaagtgtta gaacaagcat tgaaaactaa aagaattgat
    12301 ttagataaaa atatagtatt gtatgatgca aatggaaaag atgctttggt tgtagcagat
    12361 tatttatcta aaaaaggata taaatatcta tataaatatg atattaaaca atgggcagac
    12421 gacgaaaatc ttccaatgga aagatataaa aattatcaaa tgatagttcc tgcttttata
    12481 ataaaagata tattagatgg aaaaattcct gagacatttg aagattcaaa gaatataaaa
    12541 atgattgaag cgagttgggg agaagaatcg tatacaaaag ggcatatacc tacaagtgtt
    12601 catgtaaata ctgatattat agaacctcct ccaacatgga tgcttgataa tgatgataac
    12661 ttaactaagt ttgctttaga ctatggatta acaaaggatg atactgtaat agtttcaagt
    12721 agtacaccta tggcatctta tagactagct gttatattga ggtatatagg agtaaaagat
    12781 gtaagagttt tgaatggtgg aacaaactca tggttatcag caggatatga attagaattt
    12841 attagtaatc caaaacattc atgtactaat tttggagcag atataccagt taactcacaa
    12901 ttaattgtta ctacatctga attaagacaa aaactaaaag aaaaaaataa gtttatacta
    12961 gtagataacc gtacttggga tgaacatata ggaaaagtat ctggatatac atattatgat
    13021 aaaaaagggc gtataccagg agctttatat ggacattcag gtagtgatag tgtttcacta
    13081 gaagaatatc gtaatattga taatacaatg agaaataaat atgagatttt agaaatgtgg
    13141 gacaaggaaa atatagatgt gaataaacag cttatattta tgtgtggaag tggatggcgt
    13201 gctgctgaag tattaacata tgcaaatgtc ataggagtgg aaaatacctc cctttatagt
    13261 gatggatgga tgggctggag tcttgataat tcaaatttaa ttgaagtggg agaacacaaa
    13321 taaagttttt gaaagtgcaa tagagttgtt taaaacaaag gtgtttttga tttattagga
    13381 agagtgtgta aattgtgtat tgggaagtgt acttaggtat gttcatttgg agctatggaa
    13441 attgataaaa aagtattatc tgtttatttt gttggtaaaa tatgaataga ttatagatta
    13501 tagatttaag tatagactaa gtaatttata ttacagaaga gaatatataa gaattaatgc
    13561 aagatgtatt tgatacatat aagaaattgg caagtccagg agaaggatag agaaaattga
    13621 gattgaagag tttgaaagag attattagat gaaaaataca aaaatgtata aaaatacata
    13681 ttgagaaagt atatctctct taaatttgtt aataattcta ttatacagaa aaacatattt
    13741 aagggggatt ttttactatg aaaaaagtaa aattagctat aagctttttt atattaatga
    13801 ttataggaat gacaatatct tatgcagata caagtataaa tactagatac aatagattaa
    13861 gtaaagctct tgaaatatca gaagcaa
//
LOCUS       DAELJO010000090        13847 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELJO010000090 DAELJO010000000
VERSION     DAELJO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.090"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            295..975
                     /locus_tag="KPB88_002238"
     CDS             295..975
                     /locus_tag="KPB88_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0054512.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            968..2347
                     /locus_tag="KPB88_002239"
     CDS             968..2347
                     /locus_tag="KPB88_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0054513.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIKKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(2675..2914)
                     /locus_tag="KPB88_002240"
     CDS             complement(2675..2914)
                     /locus_tag="KPB88_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054514.1"
                     /translation="MRKEYGYPELEVICKASAGNEASIKEILNFYDAYICKLCLRPFY
                     HSESGKITMQIDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(2919..3338)
                     /locus_tag="KPB88_002241"
     CDS             complement(2919..3338)
                     /locus_tag="KPB88_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0054515.1"
                     /translation="MNTSSFEHIVRIQFNALMMTVIKCTVKSRNKQFARRSKREVLFC
                     ELSDMKMFEQGAEDNYSCNYTSFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SMSDQEIAELYHVSRSTVYRRRSNGLKRLKTLLKERN"
     gene            complement(3531..4936)
                     /locus_tag="KPB88_002242"
                     /pseudo
     CDS             complement(3531..4936)
                     /locus_tag="KPB88_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(5012..5572)
                     /locus_tag="KPB88_002243"
     CDS             complement(5012..5572)
                     /locus_tag="KPB88_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054516.1"
                     /translation="MRISKEPEERKQEILETAIKLFSVNGFEKTSISDIAKEIGIAQG
                     LCYRYFPSKDVLFQSAINEYADILVANMTKNINIKKDSLKDILRKMILFTEQEDDTYY
                     PVFHNNQNKNFHNLLTLNVCEKLFPIVSEIIERANCTNEIQVNDVEMYASFCIYGQLG
                     ILLNTDISTKEKSTRIKAFFRDLFRL"
     gene            complement(5596..6207)
                     /locus_tag="KPB88_002244"
     CDS             complement(5596..6207)
                     /locus_tag="KPB88_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0054517.1"
                     /translation="MNITKQKSQKTFDKQAADYDTNIQGEHARKLYKPIIENLKNKNI
                     HSILDLGCGTGALLKKIKELNIAEQLFGIDISPNMLEIARNKLGNDATLILGDSERLP
                     FEDNSFDAIVCNDSFHHYPLPDIVAKEVSRCLKQNGVFIIGDCWQPIGARQIMNYYMK
                     HSNSGDVKIYSKKEMLLLLSKDFHEIEWKSFGTTSCLIIAYNN"
     gene            complement(6555..7190)
                     /locus_tag="KPB88_002245"
     CDS             complement(6555..7190)
                     /locus_tag="KPB88_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054518.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIIDVLGVTPNELLSGEWKFVNQGEKEVCQFLKIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIVGHKYRTPHQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            7576..8739
                     /locus_tag="KPB88_002246"
     CDS             7576..8739
                     /locus_tag="KPB88_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0054519.1"
                     /translation="MINKGKNAIYTPQKNREVRKEKCTITTHENLSVKIDYISIVFET
                     TTAEDVIMHILGLPTDIFNVYPATVKFKTYQTRWEIGDIYVSGDAKKTEDNPQGLGCY
                     LVMTGKGCDDIYRILEDKGLTFGDMFGRCERRYGLDNFHFTRLDIAIDDKNENPFFTI
                     EQIKKKCEKEEFISNSEGYKFDESKFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSK
                     YNKTLDEVGSWKRTEMQLRDEKAHAFAMTFKDRPLELGELAFGLLANNLRFVVPNRNE
                     SNKSRWKTCRFWERFLGAVEVLKLQVPKQHNSLEETQQWLTEGGVISAVKSFYFLEEH
                     DALGGLEKVGTMLDKARYSNSLSSKLTAHLQRIDRTDLIPYIQYDTKHGKGGI"
     gene            8739..8942
                     /locus_tag="KPB88_002247"
     CDS             8739..8942
                     /locus_tag="KPB88_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0054520.1"
                     /translation="MNSNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEQVIDKLDAI"
     gene            9021..10025
                     /locus_tag="KPB88_002248"
     CDS             9021..10025
                     /locus_tag="KPB88_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0054521.1"
                     /translation="MKEKRRDMEDGIDSTGKKMTLCQLYAKQNAQRANVKKSTQKQRE
                     QLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFYIAIQD
                     DCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLILLKTG
                     LRISELCGLTVADIDFKNEVVVIDHQLLKSKEQGYYIETPKTKNGIRQVPLSRETIQA
                     FQRVIRKRTKGKLIEIDGYKDFLFINPKGNPKVAIDYNALFVRMVKKYNKHHEDNPLP
                     HITPHTLRHTFCTRLAGRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEVQRLIA
                     "
     gene            10230..11315
                     /locus_tag="KPB88_002249"
     CDS             10230..11315
                     /locus_tag="KPB88_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0054522.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            11369..11728
                     /locus_tag="KPB88_002250"
     CDS             11369..11728
                     /locus_tag="KPB88_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0054523.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            11781..12653
                     /locus_tag="KPB88_002251"
     CDS             11781..12653
                     /locus_tag="KPB88_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG0054524.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            12654..13097
                     /locus_tag="KPB88_002252"
     CDS             12654..13097
                     /locus_tag="KPB88_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054525.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            13112..13696
                     /locus_tag="KPB88_002253"
     CDS             13112..13696
                     /locus_tag="KPB88_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054526.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
BASE COUNT         5255 a         1949 c         2288 g         4355 t
ORIGIN      
        1 gatattaata atctccaaag tcattatttg tttttcataa ttattatttc ctttcctaat
       61 ttatgttttt ttataatatc aaaacaatgt ttattgaaag tttatataat tttaagaaca
      121 tctaaaaatg catacttgat attggtaaat tagtgatagg agttcaatgt atgatattat
      181 atactaaaga tgaattttaa tatataactt tgatataata agcatatgta aatgtttaat
      241 atttaattaa agaaaattat catataaaaa taaatttgta aggagcaata cagtatgaaa
      301 aaacaatatg tgtttataat actagcaagt actattttag gtatacttgt tggaaccata
      361 tttagagatt atagcaatga agaaacagtt tatacaagca aagacaagtc aataagaaaa
      421 gaaataaaaa tgacaagaaa atctataaaa gaattaacca aagagaaaga caagttagat
      481 aaagaaatta attcaatgaa acaaaatcaa aaaaattctg aagaaatatc agaaatagaa
      541 acacttagag atagtctatc atattcagat gtaaagggta gtgggataat attaagtata
      601 gatgctatag atgaaaaaac agggaatata gctaatagta tagactataa taagattctt
      661 gtaaaagtta taaatgaatt aaaagtaaac ggaggaaaat acatagaaat aaatggtcaa
      721 agaattaatc aatattcaga agttgtactt gcaggaagcc atataaatat aaatggagtt
      781 cctgtagcac aaccatatga aataaagtgc attggagata aggagaaact atctaattat
      841 gtagataaag gtagtaaata tttagatagc atacaaacca attatccaat gaaaataagc
      901 ataaaaactg aagaaaatat ctctatgaaa aaaatgaata tacctaataa attaaagtat
      961 ataaagggtg attaagatta tattagagga aagcagtttg ccaatgttaa taccagaaaa
     1021 acttgcaaaa aaatataatg tatttccagt tgagtttcaa gatgataatt tagttgtaga
     1081 aatagaagat gaggatattt atgctctaca ggacttgaga ttagctacag gtaaagaaat
     1141 tatattgaag aaggagaaaa aagaacttat aagtgaaaag attaaaaaat attataatag
     1201 ctttgaactt gatgaggatt atgcaaaaaa attatttgaa aatcttttag aaaaagctat
     1261 aaaagaaaac gcaagtgata tacatataga accatttaaa gaacatttaa tagtgagaat
     1321 gagggtagat ggagaactaa aggaagtatc aaagttttta gttgatgtct atccatcatt
     1381 gtctacggtt ataaaattaa aggcatcaat ggatataaca gaaaaaaggc ttcctcagga
     1441 tggtagggtg gatataaagt taggtggaaa tttaattgac attagagttt catcaatacc
     1501 aactgtctat ggtgaaaaaa tagttttgag gatacttaat agagatactt ttttaaaaga
     1561 caaattagaa cttggttttt cagaggaagc tataaaatct ataaatcgta taataaataa
     1621 aagagcagga atattattag tgacaggtcc tacaggtagt ggaaagacta caacagtata
     1681 ttccttatta aatgatttaa aaggaataaa taaaaatatt atgactatag aaaatcctgt
     1741 agagtataag atggatggca taagtcagat tcaagtaaat tcaaaagtag ggttgagctt
     1801 tgatgtaggt cttagagcga tattaagaca agacccagat ataataatgg tcggagaaat
     1861 aagagatgca gaaacagcta agatagcagt aagagcagct ataacaggtc atttagttat
     1921 aagtacttta catactaatg atgcagtatc ttccatagct agattactag aaatgcaaat
     1981 accaccatat cttttaaatg cctctcttat aggtgttata tctcaaaagt tggttagaaa
     2041 agtctgcaat cattgtagcc atgagattat gataaaagat aattttagtg gagatgtaaa
     2101 tactaaggta gctgtagggt gtgaaaaatg taatgataaa ggatactttg gaagaacagc
     2161 aatttatgaa atcttagaaa ttaatgatga tataaagaca tgtataagaa atatggagga
     2221 ttctagtact attaaagatg tagcacaaaa taatggaatg ataacatttg aagatagttg
     2281 taaaagacta ataaatgaaa aaataacgac attagaagag tgtttagtag taaatgaaat
     2341 gatttaactg aattgtatag ttaaacttcg tttggtcagt tgtctttatt taatctattt
     2401 taattaagat gtttgagtat aaattatatt taagtgaaat aaaattaaat gcattaaatt
     2461 agaaaatatt aagtttattt aattgtaaat tttatatctg attttcgatt atcagcataa
     2521 atactatact ttcaagcatt ttgaagtata gaaaaccatt cattttacca caaatttacc
     2581 acgattgaat gagccttgat atgatactaa aaaataacat gggagatagc acagacatta
     2641 gtgtatctcc cgttttttca ttcatgtaat atgattactt cactctaatt tcaaatttca
     2701 agattgcttt catcatttct gtatgaattt gaccttttaa ttcttcatca atctgcatag
     2761 tgattttacc actttcagag tggtaaaacg gacgcaaaca caatttacat atataagcgt
     2821 catagaaatt caagatttcc tttattgaag cttcatttcc agcagacgct ttacatatta
     2881 cttctaattc cggatagcca tattcttttc tcattggttc aatttctctc tttcaatagt
     2941 gtttttagtc tcttcaatcc gttacttctt ctgcgataaa cagtagaacg tgatacatga
     3001 tataattctg cgatttcttg gtcgctcatg ctttgaaaat agcgtaataa aataacatca
     3061 cgctctttat tcgataactt atataaagca acagcaagtt tctcatttga tatttggatt
     3121 gtgaaattca agacttcaaa agaggtataa ttacaagaat aattatcctc tgctccttgc
     3181 tcaaacattt tcatatctga taattcacag aataaaactt cacgtttaga acgtcttgca
     3241 aactgtttgt tcctgctttt tactgtgcat ttaataacag tcatcatcaa agcattaaac
     3301 tgtattctaa cgatatgctc gaaagaagat gtgttcataa tctcaccccc tttccgcata
     3361 cgccgaaact atataaacgg aaatgccgtc tatatctttg gaaaatacgg aaaaaagaac
     3421 aagaaaaaag tccgcacaaa acatactgtc tgtacgaact tttaatgttg taaaatattt
     3481 agttgttggt aaaatactgt taggtttctg ttgctcgtat ggaagtatgc ttaaagcttt
     3541 ccataataca cgtccttctc atagcagtta ctttcaatgt tttttatatc ttgctttttt
     3601 tagtcctatc ataaaatctc ccatataacc gcttaatgat ttgattactt gttaaaatag
     3661 ttataatgca ggcaactatg tgactaatta aaattgctat ccaaacacca tttaaatctg
     3721 tttccagcca agataaaaga taggcaagag gaatacgaat aataatataa tagaatatca
     3781 ttagtaccat actttttgtt ggttttccca tgccgtttaa agtacctaag taacaactag
     3841 taatagtgta aagaatataa ccgatactaa caattaaaaa ataatggctg acaacatttg
     3901 ttacagcttc gttggaaaca aacaattttg ccaactgttt agagaagaaa ataacaactg
     3961 ctgataatac aattgaaaca ataataccat attttaatgc taatttcaga taattctttg
     4021 ctctgtcgat acgttgtgct ccaatacatt gtcctacgat tgaagtcaac accatattaa
     4081 gagccatagc aggataaaat aatatcattt ctaattttcc tgcaactccg tagccagcaa
     4141 tagcagaaat actataagta ctaacaatag cagttaaaaa tgttgtacta atagcaggga
     4201 tactttgttg aataacagac ggcacagcat tatttataaa cggcataatc tctgttgtat
     4261 caaaatcaga tagtgaaagg ttaaataatt ttttgcgata aatataaatg cacataaaag
     4321 ccaaacaaat aatctgtgat attaaggttg cgattgccgc tccgtgaaat ccaattatgt
     4381 taatgaaaat aggatctaaa atagcgttca atagagtaga cacaagcatc gcaactgcct
     4441 gaaacatagt attgccaaaa cttcttaaaa cggctgtaaa gtataaatac aaataaactg
     4501 ctacatatcc caaaacatat atagataagt aattctttgc catatcaaaa atttcagtcg
     4561 gtgtatttaa aactatcaaa ataggttcta aaaaaatctc caatacaatt gtaataagca
     4621 ctgaaagaat tacagataca aaaaaagaag tagctataat attctttttc ttttgctcat
     4681 ttttcgcacc aattacctgt gataatagaa tggctaaccc gtttgttgct cccataccaa
     4741 ttgaagttaa aattaaaata ataggtgtag aatttgttaa cgctgcgtat gcctgctcac
     4801 ctaacaaatt tccaatccaa agactgtcta caagattata tgccatattt aaaaacatgg
     4861 ctattatcat gggaacagtc atttcaatta aacatttttt cgtgtttccg tttacaaaat
     4921 caatattttt gttcatctta attcctcctt ttgaatgaaa atcattcatt ttattgtaat
     4981 aaatattccc tgccttttgg caggggaata tttaaagcct aaacaaatcc ctaaaaaatg
     5041 cctttatccg tgtgctcttt tctttagttg atatatcggt atttaataaa attcctagtt
     5101 gtccatatat gcaaaagctg gcatacattt caacatcatt aacttgtatt tcatttgtgc
     5161 aatttgctct ttcaataatt tcagatacaa taggaaaaag tttttcacat acattcaatg
     5221 ttaataaatt atggaaattt ttattttggt tattatgaaa aacgggataa tatgtatcat
     5281 cttcttgttc tgtaaaaagt atcatcttcc gcaaaatatc ttttaaacta tcctttttta
     5341 tatttatatt ttttgtcata ttggcgacta aaatatctgc atattcattg atagcggact
     5401 gaaaaagtac atcttttgat gggaaatatc tataacataa tccttgtgct attccaattt
     5461 cttttgctat gtctgatatt gaagtttttt caaacccatt tacactaaat aatttaatag
     5521 ctgtttctaa aatttcttgt ttacgttcct ctggttcttt tgaaatacgc attgttttct
     5581 ttcctttcct tcggttcagt tattgtatgc tataattaaa caagatgttg tgccaaaaga
     5641 tttccattca atttcgtgga agtcttttga taacaataac aacatttcct tttttgaata
     5701 aatttttaca tccccactat tgctatgttt catatagtaa ttcattatct gtcttgctcc
     5761 gattggttgc caacaatcgc cgataatgaa tactccattt tgctttaagc acctagacac
     5821 ttctttcgct acaatatctg gtagtggata atgatgaaat gagtcattac agacaatggc
     5881 gtcaaaagaa ttatcttcaa aaggtaatct ctcagaatcg cctaagataa gtgttgcatc
     5941 gtttcctagt ttatttcttg caatttcaag catattggga gatatatcta ttccaaatag
     6001 ttgttctgct atatttaatt cttttatttt tttaagcaac gcacccgttc cacaacctaa
     6061 atctaatata gaatggatat tcttattttt taaattttct ataatcggct tatagagctt
     6121 tcgagcgtgt tctccctgaa tgtttgtatc ataatcggct gcttgcttat caaaggtctt
     6181 ttgtgacttt tgtttcgtaa tattcatgtg atcaactcct ttcacaaaaa ttatatctcg
     6241 tgaatgaacg attgtcaatg aataacattc aataaacttt gtgcatttca aagacgtgta
     6301 tctctaattt gaaatacacg cctttttgat tatcttacaa tcttccagtt actattctta
     6361 tgtagtacaa ctcatactgc gacacttgcg ttgatattgc ctgatttgtc acaaatcaga
     6421 ttttacttct ttcttcctct tgattaccca caacagaaaa gaaaaagccc tgtcaagagc
     6481 ttgccaccat tggtggtgct tgcactcttg attgggcttt ttcttttctg tatcttctca
     6541 caagaggaaa aagattattc gtcctcatca tcaaaatggt tattctttaa tacttcccgt
     6601 aacagttcca tatcctcttt tgagttcggg tttatcattt tcactacttg atgtggtgtc
     6661 ctatatttat gtcccactat gctttctccg aacatatcca tgctgtataa atcatcatag
     6721 cggtctagta gtttttggaa tttcaaatgc tggataaatt ctttgattgg gtaaaggtcg
     6781 gacgctacaa tgcttttccc gttgatgtaa tcggtaatat attcccgtaa cttttctaac
     6841 tcatattcca gccattcttc ctcgctatca aagaagttgt catagtgtcc atgttttagt
     6901 tctgcattta gacgttcttc gatttttaaa aactggcaga cttctttttc tccctgattg
     6961 acaaacttcc attcccccga caacaattca ttgggtgtta ctcccagcac gtctattatc
     7021 ttttccagcg tatcaaaggt aggaaaattc acacctcgtt caatcttgga aaggctctgc
     7081 atattgatac cgattttatc cgcaagttcc tgctgtttca ttcctctgtg ttttcttatg
     7141 gtttgtatgt tttctcctaa aaaactgatt ttcttttcat aatgctccat aacttagtat
     7201 aacctctcct ttcgttgctt ttcttggtat ttataagcat atcatactta atctgtatca
     7261 aaaagtcaac aaaaacttct tgacaagtaa ttctaatggc gttattatcg tatcggatga
     7321 agtgattaag cacactaggg ccaatcacac gcttgagtaa gcatagaaaa gatatatttt
     7381 cactttgata gcatgaacaa gcatggacta tcagactgaa aatcgggttt ccaaaagggg
     7441 ctggctggac ggggcacgtc ccccagacgt gtttcggaca gacagttcct tttggaaagg
     7501 gaacgccttt aggcgttcgt acagggcgta tatgtaacac cgccctgcgt atacatgtca
     7561 tagtacctag aaactgtgat aaataaagga aaaaatgcaa tttatacccc gcaaaaaaat
     7621 cgggaagtac gaaaggagaa atgcactata actacacacg aaaatttatc cgttaagatt
     7681 gattacatca gcattgtatt tgaaactaca acagcagaag atgttatcat gcacatttta
     7741 ggtttaccga ctgatatttt caatgtctat ccagcaaccg taaaattcaa gacctatcag
     7801 acacgctggg agattgggga tatttatgta tcgggggacg caaaaaagac agaggacaac
     7861 ccacagggac ttggctgtta tctcgtcatg acaggaaaag gctgtgatga tatttaccgt
     7921 atccttgaag ataagggctt gacctttgga gatatgttcg gacgctgtga gcgaagatat
     7981 ggactggata acttccattt cacaagactt gatattgcca ttgacgataa gaacgaaaat
     8041 ccattcttta ccattgagca gataaagaag aaatgcgaaa aagaggaatt tatctcaaac
     8101 agtgagggct ataagtttga cgaaagcaag tttgatgatt tcgacaccgc aaagactgtc
     8161 tatatcggag ctggaaaatc gggattgtcc taccgctttt atgacaagga taaggaagtc
     8221 tgttccaagt ataataagac acttgatgaa gtcggcagtt ggaaacggac agaaatgcaa
     8281 ctgcgtgacg aaaaggctca tgcctttgcc atgacattca aggacagacc gctggaactt
     8341 ggggaactgg ctttcggact gctggcaaac aacctgcgct ttgtcgtgcc aaacagaaat
     8401 gaaagcaata agagcagatg gaaaacgtgt cggttttggg aacgcttttt aggggctgtg
     8461 gaagtcttga aactgcaagt accaaaacag cataattccc ttgaagaaac acagcaatgg
     8521 ctcacagagg gtggcgtgat ttcagcggtc aaaagttttt acttcttgga agaacatgac
     8581 gcattaggtg gactggaaaa agtgggaact atgctggata aggcaagata cagcaactcc
     8641 ctttccagta aactaactgc tcatttacag aggattgacc gcaccgacct tatcccctat
     8701 atccagtatg acacgaaaca tgggaaaggg ggtatctgat gaacagcaac gatattcctg
     8761 tatgggaaaa atacaccctt accattgaag aagcgtcaaa gtatttccgt atcggagaaa
     8821 acaagttaag acgcttggca gaggaaaaca aggacgctgg ctggctcatt atgaacggca
     8881 accgcataca gattaaacgc cgacagtttg aacaagttat tgacaaactg gacgcaatct
     8941 aatgcaaatg agccttgtat gtgttatgat gagcacaagt catatcaagg ctctttccaa
     9001 cagggaaagg agcagacacc atgaaagaaa aaagacggga tatggaggac ggtatcgaca
     9061 gcacaggcaa gaaaatgacg ctttgccagc tctacgccaa acagaacgca cagagggcaa
     9121 acgtgaagaa aagcacacag aaacaacggg aacagctcat gcggttattg aaagaggaca
     9181 agctgggtgc taggagcatt gatacgataa aaccctctga cgctaaggaa tgggcgttac
     9241 gcatgaaaga aaagggcttt tcctacaata ccattaacaa ccataaacgc tcgttaaaag
     9301 cgtcattcta tatcgccata caggacgatt gtgtgaggaa gaaccctttt gatttcaagc
     9361 tgagtgaagt cctagaaaat gatacgaaag agaaagtcgc attgacagag gaacaggaac
     9421 aagccttact gtcattcatc aaaacggaca acgtgtatca caagtattat gatgatgtgc
     9481 tgatactgtt aaagactgga cttcgtatct cggaattgtg cggactgaca gtagccgata
     9541 ttgatttcaa gaatgaggtt gtggttatcg accaccagtt attaaaaagc aaggaacagg
     9601 gctattatat tgaaacgcct aagacaaaga acggaataag gcaagttcca ttaagcagag
     9661 aaacgataca ggcatttcaa cgggtcataa ggaaacgcac aaaagggaaa ctgatagaga
     9721 tagacggata caaagatttt ctgttcatca atccgaaagg caatcccaaa gtggcgattg
     9781 attacaacgc cttatttgtc cgtatggtaa agaaatacaa caaacaccac gaggacaatc
     9841 ccctgccaca tatcacaccg catacgctac gccatacgtt ctgcacgaga ttggcaggca
     9901 ggaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc agtatcacaa
     9961 tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag cgtctaatcg
    10021 catagaagta tttaccacga ttttaaccac gtttgataga gaaaatataa gaagatagac
    10081 ctagatatat gaggtttacc gcaaatgcaa aatgcccgta gagcccataa aataaggctt
    10141 tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat ttacaagtaa
    10201 ttaatataat ttggaaggta ttatagtaca tgagattatc taaaaatata ttttataatt
    10261 tagaaaagtt ttatttagta ttaaatagaa tcagtgataa agaaattaga gtaatttgca
    10321 aagagatagg catattgctg gaatctggat gtgaaattac aaaaatattt gaaatcattg
    10381 agagtcagtc aagtaaaaaa gtaaagaatt tgcttagtat agtatcaaat catattcaaa
    10441 aaggtaactc aatagcagaa tcatttcaaa taacaggtat attttctaaa ttctttataa
    10501 gtatgataaa agcaggagaa actagtggaa acttagatat aataatgagt gatttatcta
    10561 attattatga taaagaatac aaattaaaaa tgaaaataat aactatttca atatatccaa
    10621 taatacttat aatattatct atcttatcaa tgttgtttat attcgtattt gtaataccta
    10681 atttccaggt ggtttttacc aacaatggaa ttgaaccacc tttaataact agagttttaa
    10741 tgggaatatc tacagtggtt acaaataact tagcttatat aatatttagt tttatattgt
    10801 tttcaatagg aagcatttat ttctttataa caaatgataa tataaaaaaa ttgattaatt
    10861 cgttaaaatt caaaatacct tttataaaaa aaattaatca gttagtagct acaacaagat
    10921 tttcaagggc attgtccgtt ttaattagca gtggaataca aattgtggaa gctatagata
    10981 tgtcagctag tgttatagac aacgaattta tttatgaaag gttaaagatt tccaataatt
    11041 atataaaaaa aggaaatagc ataggtaaat ctttgaattt agcaaatatt tttccaaaat
    11101 tatttatttc aatggcaaat acaggagaag aaagtgggaa actagataaa agtctaaatg
    11161 taataaataa attttatgaa aatgaattaa ataacaaaat agagcaaata atgaaattta
    11221 tagaacctat gctaattgta tttatgggat taattattgg tacttttatt ttagcaatgg
    11281 ttgttcctat gtttgatgca attactaatt tttagagaga aaaataaatt ttattagatg
    11341 aaacttttga aaaggagaga atatttaatt gataaatttg ataaataaaa aacgaaaagg
    11401 atttacactt gttgaaatga ttgtagtagt aactatttta ggcgttatat ctagtatagc
    11461 attagttaag tatagtaagg ttcaagaaag tgccaaatta aatgcagact atacgaatgc
    11521 tgctaatata gtaaccgcag ctagcatggc aattaatgat gatgaaaaga caatagactc
    11581 tctaagtgta gagacattga aggaaaaggg atacctaaat actgttccag ttcctcagag
    11641 tacatcaggt aaattcgaac ttgtcataaa tgatagcgga acagatataa gcgtaaatat
    11701 aaattctaag caattttatc caaaataata ttttatattg attgctataa gttttaaaat
    11761 tatttttatt taagaaggag atggctataa ttaaaaaaat tctaaactta gaaataaatg
    11821 atgattatat aaggcttgta tttttaagaa aaggtcataa aaaagttttt atagataaat
    11881 caattataaa agagattaac tttgatataa aaaatgatag taattcgatt gagaagatta
    11941 agtatataga agatgtagtt agatttgtaa aagaggagat tggtaaatca aaaatattat
    12001 ataaaaattt atactttaac attcaaattc aggatatagt cattagaaat gttgaagttc
    12061 taaatactaa gaaaaaaaga gacatattaa gcatgattga atttgaaata acacaataca
    12121 ttccaataaa cataaataac tattcaataa aatataaagt tataaattct aaaaatgata
    12181 aattgaatat acaagtaatt cttttcccaa agagtattat tcaattaatt aagaacattt
    12241 cagaaaatct aaatatgaat ccaaagacta taaatataaa ttttgatata ttacaaaagc
    12301 ttataaactt aaatttaata gagaattttt cagaagaagg ggtatttata gaatgtaaaa
    12361 aaagtcagtt aattataaat ataacaaaag ataaaaaaat aagagaaaca tacttgctat
    12421 caaaagaggg aaaatcatat gagttcataa aaagattatt aagggatttt aagtatgttt
    12481 attactatgg tatagaaaat tgttttattg aagaatactt taaatataca gataaatcta
    12541 ttgaagtaaa tccattaaga ttaaaagata aagtaaaagt agaaaataat tttgatgcag
    12601 atttagaaga caataggata aattatataa atagtatagg aatgattatt taagtgaaag
    12661 tggataaaac aataaatttt cttaatggat ttaaaaaaac taaaccatgt aataaaatct
    12721 ataaaataat aatagtctta attttaacgt gtattttatt taaatcattt atgtatgtga
    12781 aatctataaa tgaacttgaa gattatataa gtcaaaataa gctagtggat aaatcaaaga
    12841 acactttacc aacaacagat aaaactagag agaaattttc taaaataaaa cttaatgaaa
    12901 taaaaaaaat atatgaaaca attggaataa atagtattac taaaatatca atagataaaa
    12961 ataagctaga gatggaaggt gtgtgtagtg atttagatgt tttaaacaat ttaaggaaaa
    13021 taaagtacat aaaatattat tacatagaca gtattgtaag agatggagaa tattatagat
    13081 ttagaataaa aagttagatg ggtgattatt tttgagatta aataatagag aaaaaaatct
    13141 acttaaaata ttgttaatat tagttattat attggcaagt gatatagcag taagttattt
    13201 ggaagaaaac ctaactagtt tgaagaatga aaagcaaaat ataataaata aaataaataa
    13261 tcaagataat tatcaaataa aggataataa gcatatagat gataaatata tagaaagtgt
    13321 tgaatcaaat aaaaatgaag aattaaaaaa taattttaat aactcatttt tagataaaaa
    13381 aacaaataaa gatatagtgt tattagtaga aaatgaactg agagatattg tgaatgtaga
    13441 atatattaat caagaaagta gtttagataa tcacaataaa aaacaagtta atttagaaat
    13501 aaaagttaat ggagaacttg caaatgtgtt gaaacttgaa ggtgtaatga agaagttaag
    13561 actaaataat aaacttgaaa agatagaaat taataaatta aaagtcaata attttgatgt
    13621 tcaaaatatt ccaaatgaat ataaacaaga aaaagacaat attgttgaat gtaaaatgac
    13681 agttagagtc atataacaaa aaagaataga taaagtaaaa aactattatc aattaataaa
    13741 attttataga ggaattagtt ttacgataat tcatttctac aagagggatt gttgatgatt
    13801 ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa tataagt
//
LOCUS       DAELJO010000091        13772 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELJO010000091 DAELJO010000000
VERSION     DAELJO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.091"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            25..471
                     /locus_tag="KPB88_002254"
     CDS             25..471
                     /locus_tag="KPB88_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054527.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            735..1460
                     /locus_tag="KPB88_002255"
     CDS             735..1460
                     /locus_tag="KPB88_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054528.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEKISSNEYSMILLDVTLPDGTGYELCQYIRVFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            1447..2691
                     /locus_tag="KPB88_002256"
     CDS             1447..2691
                     /locus_tag="KPB88_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0054529.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKLIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQVGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            2771..3448
                     /locus_tag="KPB88_002257"
     CDS             2771..3448
                     /locus_tag="KPB88_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054530.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            3449..6010
                     /locus_tag="KPB88_002258"
     CDS             3449..6010
                     /locus_tag="KPB88_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0054531.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNAEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEDDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIIIIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            6231..7505
                     /locus_tag="KPB88_002259"
     CDS             6231..7505
                     /locus_tag="KPB88_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0054532.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7666..10707)
                     /locus_tag="KPB88_002260"
     CDS             complement(7666..10707)
                     /locus_tag="KPB88_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG0054533.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYIYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEAWKKRNGKGYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEINLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSFGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            10913..11212
                     /locus_tag="KPB88_002261"
     CDS             10913..11212
                     /locus_tag="KPB88_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG0054534.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(11450..13678)
                     /locus_tag="KPB88_002262"
     CDS             complement(11450..13678)
                     /locus_tag="KPB88_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG0054535.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         5227 a         1647 c         2107 g         4791 t
ORIGIN      
        1 taaaaattag aaaggagaaa aaatatgtta acatatttaa tgaaaagaca aatgcataaa
       61 aaagatgatg gaatgagtaa accaatgatg attacaggag ctgtaattac aggtgtagga
      121 gcatatgctg tatataaaat ggtaaaaagc cataaatcta attctcaaat ggtcaataca
      181 aattattatg gaaatagcga ttatgatgat tataaatatg acgaatttga ttatgactgt
      241 gattgtagag acacaaaaca agattatgaa tatgaagaat taaaaaaaaa agtaaaagaa
      301 tttaattcaa gaagaataaa ttgtgaaaat tgtccacatg tttctcaaga agaaatgatg
      361 caatatgtaa atagtgttaa agatgtaaag gaagagattg atttacatga agatgctaaa
      421 aaagataata tttacaaaga agatgaatac aaaaaatatg aagaagaata agtaatattt
      481 tgtataactg aacaaatagt aatgtcaaat tatttaatat ctagttttaa tatgtaagtt
      541 gtaacttaac ttgaaagaat cagtatcatt ctttagatag ttaagtaaca acttacattt
      601 tatttttata aattttaata gttctttgtg actattttgt aattttagaa atattttagt
      661 atccagggta attgtgtata ataaaacttg tatagataga tagtatgaaa ttaagcagat
      721 agtttaaaat taaaatgaaa agaaaggaaa tagttatgtt aaagttgttg atagttgaag
      781 atgatagtac tatatctttt ggaataaagt atgctttaga acaagaagga tttagtatag
      841 atatatcaaa ggatttatct agtggaaaag aaaagatttc ttctaatgaa tatagcatga
      901 tactgttgga tgttacattg ccagatggaa ctggttatga gctttgtcaa tatataagag
      961 tattctctca ggttcctata atattcttga ctgcttgtga tgaagaagta aatattgtaa
     1021 tgggtctcga tataggtgga gatgattata taacaaaacc ttttagaatt agagaattaa
     1081 tatccagaat aaaagctgtt ttaagaagaa aaggtaatac ttcagaagaa aataagaaaa
     1141 tacttaaatt tggagattta agtatatata cattagaagc tagagtatat aaaaatgata
     1201 aagaaatatt tctgacatca gttgaatata aacttctttt aattcttata cagaataaaa
     1261 atactgtttt aagtagaact caaatattgg aaaaactttg ggatgtaact tatgattttg
     1321 taaacgacaa tactctcact gtttatataa aaaggcttag agaaaaaata ggtgatgatt
     1381 tatgtgaacc aatctacata gaaactgtaa ggggaattgg ttataaatgg ataggaagtg
     1441 aaaatagtgt ttctatataa tccagaagtg aagaagtttt taagtaagta tgttactttg
     1501 atgtttattg ttatcataat atctattgga tttagcataa tcaacgtgtc cttaactaaa
     1561 gagatggttg taagaaataa tcaggctata ataggaactt tatcaagcaa gtatccaaac
     1621 ttagaaagtc agatagtaga cataataact caagggaagt ctatggagaa catagattat
     1681 ggtcagaaaa tattaattaa gtataattat gataaatcta ttaggataaa ctctgagcct
     1741 attatttcta aattagtttt agagactatc aaaataaata taatattagt atgtataata
     1801 tttattttga tatttgtgtt ggttgttaga tattttaagt taatttacaa tgatttatca
     1861 gatatgacta agtatgttta ctatagttca gagggtaagc catttgatat gaaaaacaag
     1921 aatcaagaag ggcaggtagg tcttctaaaa actgaactct taaagatgac gacaattctt
     1981 aatgaaaagg ttgaactttt aaaaagagaa aagatatttt taaacaatac aatttccgat
     2041 atatcacatc aattaaaaac tcctatgact tctttaataa tgttgaatga tttattatat
     2101 aatgatgtac catacgaagt aaaaatcgat tttttaaata agataaaaaa tcaattaaat
     2161 agaatggatt ggttgattaa aagcatgctt aaattatcaa aggtagaagc aaaagtgata
     2221 aattttaaaa aagataaggt aaaatttagt gaactcatac atagagcaat gctatctatg
     2281 aaaataccta tggagattaa aaatcaaaaa ctgactatag agggaaatga taatgtatct
     2341 tatattggtg atattgattg gtccgtggaa gcattagtga atataatcaa aaattgtgta
     2401 gaacatactc ctgaatttgg aaatataatt ataacctata aagaaaatcc attattttct
     2461 gaactaataa taaaagatga gggtgaaggt atagataaaa aagatatacc acatgtattt
     2521 aaacgattct atagaggaag aagtagttca aaagaagata gtgtaggaat aggtcttgca
     2581 atgtcaaaat ctataataga aagccaaaac ggcgatatat atgtaaacag cgaaaaaggt
     2641 aagggtacag aatttcatat aatatttcat aaaatgtatg atagtgacta atctgtaata
     2701 tttaattcac tttatagtaa tagccaaaac ctaatatatg aagtatagaa tataaatatt
     2761 ggaggtagtt atggaaattt taagagtaga aaatctaact aaaagttatg gtaaaaatga
     2821 aacaaaagtt gatgctataa aaaatgtaag tttatcagtt gaaaaaggaa cgtttatagc
     2881 aataacaggt ccaagtggaa gtggtaaaag taccttatta catttattag gaggagttga
     2941 cagaccaact agtggtaagg tttatataaa tgatgttgat atatataatt taaaaactaa
     3001 ggatttggcg atatttagaa gaagaaatat aggtcttata tatcaatttt acaacttaat
     3061 tccagtctta aatgtgaagg aaaacatatt gcttccagca gagcttgaca atagagatat
     3121 tgatagagaa tattttgatg atttaatgaa aacattaggt ctaattgata gagaaaagca
     3181 ccttccaaac caattatcag gaggtcagca acagagaacc tctataggac gtgcattaat
     3241 taatagacct tctataatat tagcagatga gccaacaggt aatttagata gtaaaaattc
     3301 aaaggaaata ttagaattgt taaaattgtc agtaaaaaaa tacaaccaga cactaataat
     3361 gataactcat gacaaaaata tggcacttca agctgataga attataagca tagaagatgg
     3421 aatcataaaa agtgatgagg tgatgtagat gaatttatat acatcactaa ctattagata
     3481 cttaaaacaa aataaaagaa gaaccattgt aaccataatc ggtataatac tttcaacagc
     3541 tttaatttgt ggtataggaa atatatttga aagttttatg gattatcaaa tgagagaaac
     3601 tataaagaat gatggaagtt tccatgttac attttatgat gtaaatagaa aaaatgcgga
     3661 atatgtaacg aaatcagctg aaatagaaaa acatgcattt accaaacaac ttggatattc
     3721 taaattagaa aatagtgaaa atgctatatt aagtataaag caatatgata aaaatgcaat
     3781 aaacggatat aaagtttcta taaaagaagg tagatttcca gctaaggaag gagaaattgt
     3841 tttaagtgaa agtattatta atcttataga tgataagtta aaaataggag ataagataac
     3901 tttaaaagta ggggatatgt ttgattcaag caagaaaaag attgatagta tgacatttca
     3961 tgaagatgat tatatagcaa atgaaaaaga tagaactttt aaagttgttg gtataattaa
     4021 aaaacctggg tttgaacgtt ataatggaat tgcaacagct atagcctact ttaatcctat
     4081 gaataattac aatgatacaa ttaatgtatc agtagcagta agaaatccta aagatgtgta
     4141 taagataagt aataaaataa gtaaaaatat atattcaaat aaagataaag aagcagtttc
     4201 tgatatgata aaatacaatg aacatttatt gaggttacaa ggtgctagta agtattcaaa
     4261 tataaatagc tctataaagt ctataattat agcagtaact attttagtaa tcatatgtac
     4321 aattgctaca gtgtacaatt cctttagtat ttctataaat gaacgaaaaa aacagtttgg
     4381 gatattaaat tctataggtg ctacatctag tcaaattaaa agattagttt ttatagaagg
     4441 tattataatt agtttaattg gtataccaat aggactaata tctggaacag tagctataga
     4501 tctattgttt aaaattataa ataagtattt tactgaatcg gttgttacta agatgagttt
     4561 gcagatagtg tataatccaa taataattat tgttagtata ataatagttt tgcttacaat
     4621 ctttatatca attctactac ctgcaatatc ggcttctaat atttctcccc ttaatgtgat
     4681 taaaaatact ggagagtata aagtgggcaa aatcaaaagc tcaagactta ttaaaatgat
     4741 ttttaaaact gaaggagtac ttgcatataa aaatattagg agaaatagaa aaaaatttat
     4801 aataacactt ttttctttaa tggtaagtat aataatattt atatctttta gtggctttac
     4861 attactttta ctaagaggtg aagaaattag aaattctcaa agaaaatatg atttgtattt
     4921 aactgcaaag aacacagcta ggtctgtaga ggatactata agtgaactag aagatattga
     4981 tgggataaaa aactttgaat tggcaacagg acaatatgtg tctataagag taagtgaaaa
     5041 taaaataaat aaatccaagg aagatttaat caggaaatat tatcaaaaat ataagattgg
     5101 agatagttat gaatatgatt tcagtaataa tgaattaatt tttccaggag attttgcagt
     5161 aaaaaacaaa ataaataact tagtacaggg ctcttttaat aaagagagag caattgaaga
     5221 aaatggtgtt atattagtta gaaaaagtgc atttgaagaa aatgggaaga aaggtgtagt
     5281 agaacttacc aactataaag taggagatac agtaaattgt gaatatcttg atgaaaatgc
     5341 cttaagtaaa aaagtaaagg tcaagattct tgctataact gatgaagaac gtctaggtct
     5401 tggttatcaa aatatggggt tacaatttat aacttatgat gaagttgcta aaaatttaaa
     5461 tttaaaatta agtaaaagtc ttatatttat tgattcagga ggaagcatta agactaagaa
     5521 ggaaatagaa gctttggcaa ataaaaataa ttttaacttt tatgatgaaa gtgcaagcaa
     5581 tgaaagtgaa aaacaagatt taaaggttat taaaatattt gtatatggtt ttatagtagt
     5641 gatatcttta gtgagtgtta caaatattct aaatacagta agtacaagta taaatctaag
     5701 aaaaagagaa cttgcagtaa tacaatctat aggggttact cctaaaggat ttaggaaaat
     5761 gatatattta gaaagtttta tatatggtat actgtcattg ttgtttggaa taccaattag
     5821 tattggtata attataatta tgaataaact tatttcaggt gtaattgaat tttctccgat
     5881 tataccgtgg acagccatag ttatctgtat agtaagtgtt tttataataa cttttatagc
     5941 agggtatata ccaatgagta aattaaataa agaaaatata atagataata taagaagaga
     6001 aagtatatag atataaaaat aattcttaac tatataaaat attattaaaa tcatttaagt
     6061 ggtataaaat attattatat cacttaaatg attttttatt tatataagaa atctaataat
     6121 aaattactag aggtatttaa ctatttagaa agaattggta ttatataaag taagagaata
     6181 tataataaga aagaatattt gattttattg gaaaacaaaa gaaaaagaag gtgattacta
     6241 tttctcatat aactgcgaag aatatgtata aaagtttaga ggaaagaata aacaagtttc
     6301 cacaaggtgc tccaccatct gatacattat acaaaatact aaatgtacta tatacagaac
     6361 aagaggcgaa gttagttgct cagcttccaa taaagccttt cagagtaaaa acagcagcta
     6421 aaatttggag tgtaagtgaa agtgaagcat atagagtatt ggataaactt gctagtaagg
     6481 ctctgatact agatatagaa gataataaag ggaagaaata tataatgcct cctccaatgg
     6541 ctggtttttt tgagtttgcc atgatgagaa cgagacacga tatagaccaa aaacttttgg
     6601 cagagcttta ctatcaatat atgaatgtag aagaagattt tatcaaagat ttattttatt
     6661 caacagaaac gaagcttgga agagtatacg ttcaagagga agttttaact aatgataatg
     6721 aggttagtat tttagactat gaaagggcta ctcatataat tgatgaatca actcatatag
     6781 gtataagtat gtgttactgt agacatagaa tgcaacatgt tggaaaagct tgtgatgctc
     6841 ccatggatat atgtatgacg tttgacaatg tagcaaattc tttaataaat aataaatttg
     6901 caagacgtgt ggataaaata gaatgtaaag aactacttca tcaagcatat gaacataact
     6961 tagttcaatg tggagaaaat gttaggaaag gggttacgtt tatatgtaat tgctgtggat
     7021 gttgctgtga agcaatggta gcagctaaaa gatttggaaa tcttcatcca gtacagacaa
     7081 caagttttat accaaatata aaccatgaaa attgtgtaaa gtgtggtaaa tgtataactg
     7141 catgtccaat agatgcgata agcaaggtta aggaagatgg taaagaatat ataaaaattg
     7201 atgaggatag atgtttaggt tgtggagttt gtgttagaaa ttgccataaa aatagcatta
     7261 tgttattaaa gagagatgaa aaaataataa ctccagcaaa ttcagtacat agagcagttc
     7321 ttatggcaat tgaaaaaggg cagttacaaa atttaatttt tgataataat gctctggcta
     7381 gtcatagagc tatgggagct atactgtcag ctatattaaa gcttgaacca gctaaaaaga
     7441 ttttagccag taaacaatta aagtcagtat atttagataa attgttaagt atgaatgata
     7501 aatagaaact aacttaaaaa aggcaaagtt ttagacttat ctatatctta ataaacttat
     7561 catttaatat ttaaatttaa tttttacaca tataaaactt ataacttaaa taaaaaaagg
     7621 ctatattcaa ttaatattac ttgaatacag ccttttttag ttatgttaag ctatttcttt
     7681 tatttgctta aatgagtttt catttccatt tccaccaatt tgtgttacag atttaaatct
     7741 catagtttta aatagttctt tttgattata atctagtgag tttcctacta gcattacagg
     7801 agatttagtt tttccagcca aaactccaaa agataatccg tctattaaat catcttgttt
     7861 attcattcca tttttagcaa catataaatt gtctatctta gaatccttgt aaaattcttt
     7921 tattactttt gcattagttt catttctagt gctacctgat attctctgtg gattttgaag
     7981 tttactttct atatttttag aaactgtata ttctccaccg attacataag atttatcaat
     8041 ctttttgttt ttaaacaagt tatttatttc agttatatta ctattttcat tagtaagtat
     8101 tataggcata tcattttgag ctgatacagc tccaatgctt actgcatcgg ctaatccttt
     8161 ttcgccatta actactgaaa tctttgatat attgctaata ttgttcaatt ctttagcaat
     8221 gttcatagaa gtttgatatc tatcacttcc tgaaattcta gatacagaga tattgtttga
     8281 ttttatagta tctaaagatt tttcatttat agaagcttct ccaccaacta catatacatt
     8341 tttaactttt aatctcttta attcagccaa agttttttca tttatttgag agcttccagt
     8401 caataatata ggagcgtttt tcttgtatgc aaatggtgtt gcggccaaag catctgcaat
     8461 tgctgaatcg tttattaaaa ctgcattttc agcattttcc caacctgctt gacttatctt
     8521 aatagaagtt tcatatctat cagttcctgt aagtgcactt gcatttactt catcttgata
     8581 tgtaacctca aatctagcat ttggacttcc taagaaaaca attacgcctc cttttggcaa
     8641 tctaactcta ttgttctttg taactaaaga atagtttaca ggaacacctt tatcatcata
     8701 aacagcaaaa gatgaatttt gaggaagatt aatttctatt tttttatttg cgatatcgtc
     8761 accaatctta taccattttg cataaccatt ggattctagt tcacaagtaa aagatttttc
     8821 tgctggcaaa ttagttatag aatcttcact aacatacgtt agtgttccaa aagataaata
     8881 ctctgtacca ttttctttat gaagtttaat gtcggataaa tcacgaccta ttacaccagg
     8941 tatttctata aaagcatttg agttattctc atccattatt ttagtgttaa ctatataacc
     9001 tggagtttca tcagaaagag caagagttgc cttcacagaa ccaaacatat agctttgaga
     9061 agtatatttt tcatctacta gatagtaacc tttaccattt ctttttttcc atgcttcttt
     9121 tacactattt gatatattgt tgtcatctac tttttgagct acatagtata aacttgctgt
     9181 ctgacctaat ccagggacat catcataaga gctcatattt aaatatgtga tattattttt
     9241 ttcttttaca aattttaaac aactattacc tttttcagat acaaatctat cttgacctat
     9301 gtatatgtat ttatcttcag gtccattttc tatataagga gaagatactg ttaaagtacc
     9361 attatcatta acatctactt ttatcatatt tgaagaagca taaagaccac tattttcttt
     9421 taaagagctt ggcattttaa cttgttgtgg tttactaaat gttttatcag gttttatttc
     9481 tttaatttta cctttttctt ttaatgcaga tagtaagatt tcttgtccaa ttatttcgtt
     9541 aagttgacta cttccaccag aagaaagaac tgcaactgcc atattttcat ctggaagaac
     9601 tataagatta ctgtggaata ataaagagtc tccacctttt gtaagagctt ttaaattata
     9661 ctgattaaat ggataagtgt tcacacaatc ccaccctaaa ccatatccaa gtatagagtc
     9721 ttctccttca ggccatagac catttaaata ttctttgttt tccatagctt ttactgaagc
     9781 aggagaaaga ataccatttg agtttttcat aaatgtttgt gcaaatgtac ataggttttc
     9841 tgcacttgaa tataaaccac ctgcacctat agcattaagg ttatcttgtg gaactgcatc
     9901 ttcccaataa ggtacatatg cttttgctag ttttgaacta tcaaaactat tttctggagt
     9961 ttttgtgttt tgtaagttaa gtggattatt tatgtattta tcaagaaaat tagtaaatga
    10021 cataccagat actctctcta ctaaaatttc agctaaagta aatccatcat tacaataaac
    10081 agaaaaagct cctggtttag cttttaatct ttgtttttgt aattctttta agaaattatc
    10141 atgaccataa gaatcattgt ctgctaataa aatagtgttt ttaaaactac ttcccatcaa
    10201 ccctgatgaa tgatttaata acattcttgg agtgatttcc ttgtatctat catcagccat
    10261 tttaaattca ggtatatact ttacaacagg agtatctaaa tttaattttc cgtcatcaac
    10321 caacttcata acagctgttg tagtaaacat tttactgata gatgcgatac tatacatatt
    10381 atccttattt aagtttttgt tatcctgttt gctgtaaact ccaccattac cagaaatctc
    10441 aattttgcca ttatcaatca aagcatattg agcacttaca gcaccatact ttgatagcaa
    10501 agtttggatt ttctctttag caagagcttt agtttttgtg taagattccg cgtttttact
    10561 ataatcatgt aattctatat cagaactttg atatttttcg gaattatctt ttgtattatc
    10621 agcaaaagta atatttggag cagcagtaaa taacatactc aatactaatg tactagataa
    10681 taatttagag agcctttgct taaacatatt acattacctc tttccctttt taataattta
    10741 taattagcat aacattctta attattttaa tcaacagatt attatcaata aaaaaagctt
    10801 ttataagtat agctttttat ataacagaga aaaatataaa aataatattt gttgttgtat
    10861 aaagaataaa gggtataggt aatatataaa taaaatggag gaattttaga caatgtataa
    10921 gaatataaat aagaagcaac ttaaagaaat gatgaaaaat gaaaaggatg ttttattatt
    10981 agatgtaaga actaaagatg aatttaagga atataaaata gaaaatgcta taaatatatc
    11041 tttacaagag ttaatcaata atatagatga aatttacgat tataaagata agaaagtagt
    11101 agtttattgc agaagtggtc atagaagtgt tactgcatgt aatttgttag ctgaagaagg
    11161 ttttgagcat ttatataatt taaattcagg aataattgat tatatgagtt aaataatgta
    11221 agttatacat taaatactat gtatagctct gcttattaag taggaatata tctaaatatt
    11281 tataagataa atttaggaaa gaacgaattt tttcgttttt ttctatgaca gtttttgtag
    11341 taaatattag tttttaagtt ggaaaaaggt agtagctttt tagctactac cttttaatat
    11401 gaacttagaa tattattaaa aaaatatatt taaagacaga attaattttt taatgaccag
    11461 ttaaattact atcgccgaat ttatcattaa atggttttat aactctattt ataggtcctt
    11521 ttagaagtac atttaaacaa atagacagaa tcataaatag taatagaact agtatatctt
    11581 tagttagatt tggagcatat ataccaccaa ctgcttccct tgtagcacct attgcatagg
    11641 taaatggcat tatagggttt attctttgga agaatgtagg tgtaacttgt atagggaatg
    11701 taccaccaga agaaccaatc tgaacgacta atagtactat tgcaattgct tttcctacat
    11761 ttccaaatac agaaactaaa gaatacacta taaagttaaa taccatacta gtaaacataa
    11821 gcagaactat caataaaatt ggatgttttg ctgttactcc taacaataat atatctccag
    11881 ttcctattat aaaaccttgg acaagagcta tacttaaaaa agtaaggcct cttccaaagt
    11941 aaatttcata aggtttatag tttccatgta cagatgtaga caatagtgct gaaataagta
    12001 atataccaac ccatgtagaa agaacactat aaaatggtgt cattgcagaa ccataatttt
    12061 caattggata taatttctct gttttcacct caacaggttc ttttaagaaa ttagactttg
    12121 tcaatatatc actttcaagc aagctgacta attttttcat atcttcacca ttactaattt
    12181 tgcttaaagt atcaatcaaa tcatccaaca ttccttttgc taaaggaagt ctttctctta
    12241 tcaatgatat actttcctgt gcatttcctg aaagttttag agaagtagta agtatttctt
    12301 caacctttgg aagttttgct tctgccttat ctaaaacagt aattatatca tttgctactt
    12361 ttatactgtt tgcaaatata ctatttatag gttttgatat ttttgagtca aaattattta
    12421 aaatatttaa attaattctt ccaataccat ttgatagact taaaacatta ttcaaagcac
    12481 ttatagatgg ttgttgtcca gatataactt tgtttttaat atcatttaat gttgatatag
    12541 atgaatctat tttattgcta gagtcttcaa gattatctat tacatcatcc aatctattat
    12601 ttgatgtaag ttggtttaat ttagttaaaa agtccaccaa agtatcgttt aaagattgca
    12661 aattagaaag tttttctgat aaattatcta tcaacttagg tacatcttca ctgccactat
    12721 ttactgcatc aattaaattt aatgtaagag aagaagcaga agatgaaagg tcaaccatta
    12781 aatttaagtc agacttaata agaggagaaa gttcgtctaa attatcattt gtatcctcta
    12841 agaatttttt taagtcactt gataatagtt ttgtatcatt cagagtcttt ttaattgtag
    12901 gtaaatcaga tttaatatct ttaactgtat cagataattt tgaagtagca tcagaagcta
    12961 aatcaacagt cttatttata tggtcaaatc tcttttgaag gtcaataagg gaattttcaa
    13021 cttttgtcaa ttttggaaga ctgtgttcaa ttcctgcacc aactccgttt gatataccaa
    13081 gaacgatttc actaactgtt tttactatag tctgattaac ctgtagttgt attgtagaag
    13141 cacctttatc tgtaatcttt ggtgcaactg catttatttt ttcattaaca gtatagataa
    13201 tttcgccttt tttaacatca tctgtaatta aagatgtcaa atcttttgta aaattttttg
    13261 gaatcttaag tgatgcataa taagtacctt ttttaacacc ttcaagagca gttttttcat
    13321 ctacaaactg ccaacctaaa ctcttgttat ctttgagttg ttcaattaat tggtctccta
    13381 cattaatctt ttgtcctgct atttcatttc ctttatcttc atttgttact gctacaagca
    13441 tattagcagt gcttccataa gggtcccaag atgcctttat attgaaccaa gcatacagag
    13501 atggtaaaaa agcaagacct aaaactatta caagtacggc ataattgcca cgtatatctt
    13561 ttaggtcact tttaaaaatt tctaatatat tttttaaacc ctttgaaggt cttaaattct
    13621 tgatattatt ccagtttaaa atttctttta aactattagt ttttttcaaa tgtctcacct
    13681 cctaaaatat tttgataaag aaaaaagtta gatttataat caattttaaa ttatattact
    13741 ttttacacaa atagtaaagt taaaattttg tc
//
LOCUS       DAELJO010000092        13768 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELJO010000092 DAELJO010000000
VERSION     DAELJO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.092"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            84..419
                     /locus_tag="KPB88_002263"
     CDS             84..419
                     /locus_tag="KPB88_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054536.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            556..1833
                     /locus_tag="KPB88_002264"
     CDS             556..1833
                     /locus_tag="KPB88_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG0054537.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1954..3346
                     /locus_tag="KPB88_002265"
                     /pseudo
     CDS             1954..3346
                     /locus_tag="KPB88_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            3717..4430
                     /locus_tag="KPB88_002266"
     CDS             3717..4430
                     /locus_tag="KPB88_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG0054538.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4548..5417
                     /locus_tag="KPB88_002267"
     CDS             4548..5417
                     /locus_tag="KPB88_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0054539.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5572..6717)
                     /locus_tag="KPB88_002268"
     CDS             complement(5572..6717)
                     /locus_tag="KPB88_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0054540.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7051..7827)
                     /locus_tag="KPB88_002269"
     CDS             complement(7051..7827)
                     /locus_tag="KPB88_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0054541.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8409..9383
                     /locus_tag="KPB88_002270"
     CDS             8409..9383
                     /locus_tag="KPB88_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0054542.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9481..9786
                     /locus_tag="KPB88_002271"
     CDS             9481..9786
                     /locus_tag="KPB88_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0054543.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9942..11102
                     /locus_tag="KPB88_002272"
     CDS             9942..11102
                     /locus_tag="KPB88_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0054544.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFVNMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11152..12054
                     /locus_tag="KPB88_002273"
     CDS             11152..12054
                     /locus_tag="KPB88_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0054545.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12134..13039
                     /locus_tag="KPB88_002274"
     CDS             12134..13039
                     /locus_tag="KPB88_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG0054546.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNICQLTK"
BASE COUNT         5339 a         1522 c         2368 g         4539 t
ORIGIN      
        1 agctaggtat atttataata ttaataaata tattgctata aaagtttgtt taataaatta
       61 aataaagaaa agaggagaat aaaatgtaca acatagatga tatgatatat aattgtccag
      121 tagaagcttt atcaaatatt ttaggaaaaa aatgggtggc tataattata tggaaaatac
      181 aagaagataa aaaacgattt ggagagctac aaagagctat accagactgt agtaagaaaa
      241 tgttagttca acaactagat tttttaattg aacaggggat tatagtcaac caaaaaaagt
      301 tggttaataa tgttgtagag tcaacatatt ttttaagtga atcaggatta cgattgcttc
      361 ctgtcatgga aaaaatgatt atgtggagca ataaaaattt aaattgtgat aaatactaaa
      421 atttactctg taagtaatga aaatttaatt aagctatgag tagaaaaatg tattaaaatt
      481 acataatttt ttagttagat tttaatttag aaatgggatt gtaatattgg aaataaaata
      541 aggaggaatt ttaatttgca aaatgtaaat ttatttttag tatttataga aggtatagta
      601 tcattctttt caccatgtat actgccaatt ttgcctattt atttgagtat attgtcaaat
      661 agtagtgtag aaaatttaaa agaaggaaag acgagtttta taggaagttc tttatttaaa
      721 aatactatat tttttgctct tggaatctca acaacattct ttatattggg ttcatcagta
      781 aaagtattga gtatgttttt taatgaaaat aaagatttaa taatgtttat tggtgggatt
      841 ataattatta tcatgggatt attttacatg ggaataatta aatcttcaat attgaataga
      901 gaaaaaagat ttaatgttaa gtttaaagaa atgaaagcaa taacagcttt tatattaggt
      961 tttacattta gttttggatg gacaccttgt attggtccaa tattggcatc tgtattagtc
     1021 atggtttcaa gttcaagtaa tcatcttagt gcgaatcttt taatagcagt atatactatt
     1081 ggttttattt tgccattcat aattacagct atgttttaca gtaaactatt taaaactata
     1141 gataagataa aatctaacat ggaaattatt aaaaaaatag gtgggattat acttatagta
     1201 tcaggaatat taatgatggt aaatggattt ggaagtataa gcaaacactt taatacgtca
     1261 caaaatagta aaattgaaag caagcaagaa gaaaataagc gagaaaattc tactgataaa
     1321 gaagaaaatt ctgatggtaa tgatagtcaa aaagactcta ataatgacaa taatgataaa
     1381 gggtctaatg atgaagatag aataaagtct attgatttta ctttaacaga ccagtatggt
     1441 aaaacacata aattaagtga ttatgaaggc aaagttgtat tcttaaattt ttgggctaca
     1501 tggtgtcctc catgtaaaga agaaatgcct tatatagaac aactgtataa agactataat
     1561 aaaaataatg atgatgtagt aattttagga gtagcttcac ctaatttagg tagagaaggt
     1621 tcaagggaac atgtagttaa cttcttaaaa gaccaaggtt atacattccc tgttgtactt
     1681 gatgaagatg gagcattagc atatcaatat ggaattaatg cttttccaac tacatttata
     1741 attgacaagg aaggatatgt aactcagtat attccaggag ctatggataa ggcaactatg
     1801 gcatcattta ttgaaaatca gagaaataaa taaaagtatt taagtataag tttataaatc
     1861 aatttaaggt tacaaaaaag tgcctaattg taccacctaa ataatcttgt tatagtaaaa
     1921 gtataaacaa tacaagaaag ggttgaattt tatatgaaaa aaacatttga tgctataata
     1981 ataggatttg gaaaaggtgg aaaaacatta gcaggggatt tggcaaacag gggattaaaa
     2041 gtagctttaa tagaaaagtc aaacaagatg tatggtggaa catgtgtaaa tgtggcatgt
     2101 ataccaacaa aaagcttaga aaattcagca aacagcgtaa aaacaaagaa tataaattct
     2161 tgggatgaag tacaagctga atatgagaaa gctatagata aaaaagaaac tttaattaca
     2221 aaactcagag aagctaacta taataaatta aattcaaatg agaatgtaac tatattcaca
     2281 ggaatgggaa ctttcattga tgaaaaaaca gttcaagtca aaactgaaaa tgaaatttat
     2341 gagttagtag cagataatat atttattaat actggttcaa gaccatttat acctaatatt
     2401 aagggaattg aaaataagaa tatagtatat gatagtgagt ctcttatgaa tctaagaact
     2461 ttacctaaaa aaatgacaat tataggagct ggatttatag gtttagaatt tgctggtatt
     2521 tatagttcat ttggagcaga agttacaata ttaaactcta ataatggcat tttaccaaat
     2581 gaagatgttg aggattcaga agaaataata aaattacttg caaagagaaa tgtaaaaatt
     2641 gttaataatg caaatataaa agagattaaa gaagtttctg aattagcaat agtagaatat
     2701 gaagtagatg gtaaatctaa agaattaaca agtaatatga tacttgtagc tacaggaaga
     2761 aaagcaaaca ctgaagggct agggctagaa aatgcaggta tagaattaaa tgaaagagga
     2821 tttattaaag tctcagaaac tcttaagact aataaagagc atatatgggc tataggagat
     2881 ataaatggag gtccacaatt tacttatatt tcattggatg attatcgcat agttattaat
     2941 cagttatttg gagataagac tagaacaact aatgatagaa agaatatccc aaattctata
     3001 tttataagtc cagcattttc aagagttggt cttaatgtta agcaagctaa agaaaaggga
     3061 tatgaagtat tagtggcaaa gatgccagta gaagcaattc caagagctaa acagattggc
     3121 aaggcagatg ggtttataaa aatagtgata gataaaaaat ccaataagat acttggagct
     3181 agctagtatg atatgtgaaa attcaagtga gattatccat cttattcaat tagctgtaga
     3241 tttagaagta gagtatacat atttaagaga ccgtgtttat gcacatccaa ctatgacaga
     3301 agctcttaat gatattctat ctcctaatat gataaaagaa gtataacata agtttaatat
     3361 ataaatctaa aattatcatt ataagtagat tttagaaaga ggctatctca aaaaatagag
     3421 ataagtctct tttttactgt ttaaataata aaaatatatt tgattttata aaagaagatt
     3481 attttatgag ctaaaaagtt tatttagaaa cactacctat ttatgttata aagttataaa
     3541 tataatatag aatctattat tctgtattat taagtggggt taagcgttat aattaattat
     3601 aaatattgaa taatctaaat ggataagata atggtattaa aaattatctt aaaaggtgaa
     3661 ataatctgta tttaatgtag tacagttata tctttaaagc aaggtggtga aatgatataa
     3721 ataagatatc taaaagaggg tttgatgata gtgataaaag atggttttca agtaaagaat
     3781 taacaagact tataaaggct aaagaagaaa tagaatggtt attaaatagg gattacaaaa
     3841 tagacccagt cgttactttt gttggtgata gatatcagtt ttctataaga caaagagatg
     3901 cattaaaaag ggcagtctgt actgatgaaa aaaatattat aagacaatca aagaggcttt
     3961 ctctagataa aatcaaagaa gataccgtaa atatagatgg ctttaatttg ataatatcca
     4021 tagaggtagg attatctggt ggtactctag taataggaag tgatggcaat ataagagatt
     4081 tagcaggtct taggggaacc tataagatta tagataaaac agaagaagct ttgaatctga
     4141 taggtaaatt ttttaataaa tacaatgcac agaatcttaa attttattta gatgctccag
     4201 tttctaattc aggaaattta aagtatagga tattagagca tgccaaaact tggggaattg
     4261 aaactgaagt agaattagta aaaaatgcag atgttgtatt ggaaaaatta gatagagtag
     4321 ttagtagtga tgcagtcata gttgacaagt gtataagtta ttttaatgtg gctagaggta
     4381 taattgaaga gtatattaaa gaatgtaata tcataaattt aaataaatag ataaatatag
     4441 atgagaatat ataagtatat gtagcaatat tattagtaat acaagtaaag tttgaaaata
     4501 ggagaacgaa tacatatcga atttcataaa atgttgtaaa aagacatatg agaggatgga
     4561 tatgtgtgaa attaactaaa ctaaatatta aaaaatatag agcacctata tataaatatg
     4621 ctttagcaaa tgtaaatttg aggtctgcta aatctactaa ttcaagtatc ataacagtaa
     4681 tacctcaggg agctaaaatg gaagtattgg atgaagaaga tgactggatt aaggtgatgt
     4741 acaattctca agaaggatat gtgtataagg atttggtatc agtaagcgaa tatgcttgga
     4801 gtaacttaaa cttaagagaa gataaatcta caacttccaa tataattaca gtaattcctg
     4861 aaaaatctag ggttgaagtc cttcaagtag atggagattg gagtaaggtt gtatatgatg
     4921 ataaaatagg atatgttttt aattattttt tatcaattga tgggaataaa ccaaatgaat
     4981 tagattataa gtatttttat actgacatga ttaaatttgt taacgaaaat aatattaaaa
     5041 gtactagcga ttatctaatt gtaacagacc ttagaaataa atatacttat atttttaaga
     5101 aagataatgg aggctgggga caactttaca agtggcaatg tactataggc aagcctgaga
     5161 ctccaactat aacaggtata ttttatataa gtggaagaaa accttctttt ggaacggatg
     5221 agtattcagt taagtatgct actagaatta aaggtggata ttactatcat tctgtactat
     5281 atgattcaac aggaagttat ataatagatg gcagacttgg tgaagctctt agtcatggat
     5341 gtataagatt aagtacggaa aatgcaaagt ggatatatga taatatacca gatacaacaa
     5401 cagttataat tcattaagaa aaattttgag ttttttattg ggataatgat tcttaaatta
     5461 ataggattat atattaactt ctcataaaga gtactattta aaaagtgtaa taagagagtg
     5521 ctattaacaa aacaaagata ttttgaggca ctctcttata aatatacata attaagatat
     5581 tttcataaac aattctataa tcttatcttt atcaaagaaa acaggattag caccagcaca
     5641 agcatctttc atagagtttt ctgcaagcaa ttcaaaatta ggattatcaa cacctacatc
     5701 ttttaaagat tttgggattc caacctcttc agaaagagct tttatcttat ctattacaaa
     5761 gtcaacacac tctttatctg atttattatt tacatccatt ccaataactt cagctattgg
     5821 tcggaatttg gcaggggctg atttagcatt ttcctcctca actattggaa gtagcatggc
     5881 attacatact ccatgaggta aatcatataa acctcctaat tgatgagcca tagcatgtac
     5941 atttccaaga cctgcattac taaaggcaat tccatttaaa aaacaagcat aacacatttg
     6001 ttctctagct tctatgtcat taccattttt aacagctctt ggaaggtatt taaatatctg
     6061 ctttatagca tataaggcag ttgaatctgt aacatcataa gcaccattag ctactacagc
     6121 ttctatagca tgagttagtg catccatacc agttgctgct gttaaattag atggtttgct
     6181 aatcattaat tctgggtcat ttactgtcat tgtggctagt gaattggtat caaccattat
     6241 catctttaca tgtctgtctt catcagttat aacatagttt attgtaactt cagcagatgt
     6301 accagcagta gtagttatag ctatgatagg aagacatttt ttagaagttt tgttgatacc
     6361 ttcatagtct ctaatatctc ctccattagt ggctaatata gagatagctt taccacagtc
     6421 ttgtggagaa ccacctccta tagttataac gaagtcgcaa ttttctgatt ttaatatttt
     6481 aagtccttta ttcacattag ttacagttgg attttgttta acttcatcat aaacaacaaa
     6541 gtctacatct atctgagata aaatatctgt tacttttttt acagtaccat ttgaaactag
     6601 aaatttatca ctaacaataa atgctttttt agttcctaat gatttaattg aatctttagc
     6661 ttcattaaga catcctttcc caagtaaatt tattggtggt acataataaa tgctcataac
     6721 taaaatctcc ttttattttc attgattttt atgtaaaaat atatatctaa caattttcac
     6781 taatttactc ataatatatc gtatcttaga ttgagatagg gttgctggtt cttatataag
     6841 aacttaattc gtaattacga ttatgattag aatttaacat agtattttta tcatgtcaat
     6901 aaaaatataa aaaatattat aaaaaaaaac aaagttactg tagttaaatc agcaactttg
     6961 tttttatagt ataaatcaaa taaatacatt agtttaaaac aaaattttta attattatgt
     7021 tttgatatct tccatataca gaatattaat ctaatttatt acaatgtctt cattacgttc
     7081 ttccatcatt gattgaatat ttttctgagc atttaagagt aattttttta tcagttctat
     7141 tttttcgtca ttagctctaa ataaaggcag tgttacacta acagcagctc ttatacatcc
     7201 attttttctt attggtacgg caagacattt taggtgttcc attgattcct cacactctga
     7261 agctatttgt gttctttgaa cttcaagaag ctggtcgtat aaaacatcaa aatctgtaat
     7321 tgatttttct gtaaaacctt ttagaccatc tggatataag cttattagtt tttccttgtc
     7381 aaaatcacat aaaagagctt ttccaagacc tgtacaatta gcaggtattt tttttccaac
     7441 ataagaaata agtctaattg gttctggaga atctactttt ccaatataaa gaacttcatt
     7501 attgtcaagt ataccaagct gacaagtttc agaacaggaa ttaactatat tagtcatttc
     7561 gtcaattata tagttaaaaa tagttctttt ttcaaaaaat gaagacccta atgtgtaaga
     7621 acgtatccct atagaatatt tggatgtgtt tttatcaata gtaatgtatt ttctagcatt
     7681 aagtgtatga actataggaa agatgctact ttttggagca ttgataacat ctgctatttc
     7741 tgtaagagta tatccatttt cggattctgc taatgcttct aaaatatcta atatcctagc
     7801 tgttgggcga tgattatctg aagtcataaa tttccccctc tttttattaa tttaaagtat
     7861 tagaatatgt tgattttagc acactatacg atattttaca atgtagaaaa taaatatgta
     7921 aatttatata taaataagct cttgatttag atacagatag atattttttt agactgtaca
     7981 ttaaggaata actaatttcc aaaatatacc taaagaaagt aagtgatatt tagttatata
     8041 atttaagtaa atatatgcaa tagttatttt tatatgtaaa tatcaaaata ttatgaactt
     8101 atattttatc tattttttac actgtataaa aactttatag ttttgaatac tatataattt
     8161 taaatacgct gtatttttgc atataaggaa gtcatttgat aatatacaag gtattatcag
     8221 aaagtttgaa aaaattttga caaaaattta ttttttttat tgactcataa ataggaaaat
     8281 gctatcatta attcaaagat tcgtaattaa aaacttagtt cttgaatacg aaccgaaacg
     8341 gccgttagaa ttaaagctaa aaacttaata agagtaatta aatatcatgt tttaggagga
     8401 ttaactttat gataatggat tttattaaaa aaattcctgc aggtatgatg atagtgccaa
     8461 tgtttatagc agctttcatt aatacctttt gcccagatat tgtacaaata ggttcgttta
     8521 cgacagcagt attttcaagt gttgggtctg caactataat tggggttcaa ctggtatgct
     8581 tgggaactca gcttcaattt agagaaatgc caaaagtttt aaagagaggt ggtatacttc
     8641 ttggttctaa atttgctata ggtgctttga taggtatact tataggaaag ctatttggaa
     8701 tggatggtgt ccttggtctg acaacattgg ctgttataag tgcagttaca aatagtaatg
     8761 gtagtatata tctttcattg atgacttcat atggagatga aacagattgt gcaactatga
     8821 gtttacttac tttaaatgat ggaccatttt ttacattaat agctcttggg acttcaggtc
     8881 ttgcaaatgt accattgttg tcattattgg ctgcaatagt gccaatactt gttggtatga
     8941 taataggaaa ttttgacaaa aaaatgaaag agtttttaga gccagggtgt aatttactcg
     9001 taccttttgt agggtttgca cttggtagta gcataaatct tgcaagtata ctaaaaggag
     9061 gtttatcagg agtattactt ggattaatat ctgtatttgt aggaggtata tttatagtat
     9121 tctgtgataa atttatagga aaacgtccag gatatgcagg atgggctgta gctacaactg
     9181 ctggaaatgc aattgctgta cctgctgctg ttgcattagt tgacccatca tggcaaccat
     9241 atgttgcaac agctacaaca caagtggcag ccgcagtagt tgtcactgca atattagtac
     9301 ctcttataac tagttggtgg gctaagaagt atggatgtcc taagttccca aaacaaaatg
     9361 aggatggtac aataacagct taatttatat tacagtgagt tgttggtaaa ataataaatt
     9421 ctttaattag attttaggat attaataaaa ttatattatt tataaaggag agcaagaata
     9481 atgataaatg cagtgataat tgatgaaaaa gataatgttg cagtagctat tgaagcaata
     9541 gctaaaggta gtgaaattag ttttaaatta aaagacaaaa cagtaaaaac gattacagca
     9601 ctagatgata taactattta tcataaattg gctataaaag atatggccca aggtgataaa
     9661 gttactaaat atggtgaaca tataggagaa gcaaatcagg aaataaaaag tggtcaacat
     9721 gtacatattc ataatgttag aagtgttaga gaggatttgg ataaacaagt tatgactaat
     9781 atataaaata gtatttatag agttataaaa gttgtattca ttgcttttag tgtcaaatag
     9841 tttttaaata ggattttagc tgtttgatta gtgagtgtaa taaaaattat aatatataaa
     9901 aattataaaa agtatataga ctattttagg aggaaatcag tatgaaattt tatggatata
     9961 aaagaccaga tggaagagtt ggtataagaa accacatatt aatcttgcct acgagtgtgt
    10021 gtgcatcaga tacaactaga ataatagcat cacaagttca aggggcagtt acctttaata
    10081 atcagaatgg atgttcacaa gtacattcag accaacaatt aactatggat gttatggctg
    10141 gctatgcagc aaatccaaat gtatatggtg taatagttgt atctttggga tgcgaaaatt
    10201 gccaaaatga tttggtagtt gatgcaataa aagagagaac aaataagcca ataaaaactc
    10261 ttgtaataca agaagaacat ggaactttaa aaactataga aaaagctgtt cgttatgcta
    10321 gagaaatggc acaagaagct tctttgctta gaaaagaaga atttccaata tctgaactta
    10381 tactaggaac tgaatgtggt gggtctgacc caacaagtgg attagcagca aatccattaa
    10441 taggtgaatt aagtgataaa cttgtcgatt taggagcaac atcaatttta agtgaaacta
    10501 ctgaatttat aggtgcagag catatactag ctaggagagc agtaaatgaa gaagtaaaag
    10561 aaaaaatatt acatatagta catagatatg aaaattcatt aaaattggtt ggagaagaag
    10621 ttagagaagg taatccatct cctggaaata tagctggtgg tttaacaagt ttagaagaaa
    10681 aatcacttgg ttgtatacat aaaggtggtc atagacaaat aagtgaagta tatgattatg
    10741 ccaaacaaat tgataagaaa ggtcttgtta taatggatac ccctggaaat gatgcttcat
    10801 ctgttgctgg tatggtagca ggaggagcac aaatagtggt atttagtaca ggaagaggaa
    10861 caccatcagg aaatccaata gcaccagtaa taaaaataac tggaaataaa ataacttttg
    10921 taaatatgga agataatatg gattttgatg ctagtccagt tatctatggt ccacaaacaa
    10981 tggaagaatt aacagatgat ttgttaaata tggttgtaga tgtagcaaat ggaaaacaat
    11041 caaaagcaga gtcccttgga tatactgaaa tggcaatagc tagagtatgc aactatgtat
    11101 aatctttaag taagaaaata aatacataga attttaataa agggtgagat tatgaagttt
    11161 gtaagtttta ctgaaggaga aaatgaatgt aaaagaactg gagttttttc taaagatgaa
    11221 ttatttatta tagatgttaa ctctttaaat ctcagtagaa attttaagga tttaaatgaa
    11281 ttaattcaaa atgtatctag tgaagatatt attaagttga aaactattat aaatgaaagt
    11341 acattctata attatgatgt ttttaaaaga gagagatgta ctgtacatgt tcctatagaa
    11401 aaaccaatac atgatatctt atgtgtggga gtgaactata aagaccatct tgaagaaact
    11461 caaactcact ttgatgatag ttttgaagaa ccacaaaaaa cggtttattt tacaaaacgt
    11521 gtgacaaagg caataggtcc agaagatgag atagatggtc attttgagat aaataatcaa
    11581 ctagactatg aagttgaact tggagtagta ataggtaaaa caggtgttaa tattagaaaa
    11641 gaagaggtag aagattatat ctttggttac actgttataa atgatatttc tgctagaaag
    11701 ttgcaaaaag aacatgttca atggtttaga ggaaaaggat tagatacatt tacctcttta
    11761 ggtccatgta ttttacataa aagtgcagtt ccttttccca taaaacttaa agtatgtagt
    11821 agggtaaatg gtgaagaaag acaaagttct aatacaggat tattcatatc agacgtttca
    11881 gacatagttt cagaactttc aaaaggtatg actttagagg ctggagacat tatagctact
    11941 ggaacacctt ctggagtagg gatgggattt tcacctcctc gatatatgag tagtggagat
    12001 gtagttgaat gtgagattga gagtattgga attttaaaaa attacattaa gtaactgttt
    12061 taatataatt aatattaaag ctaaatctga tgtattaatg aacatattaa aataaatttt
    12121 aggagtttaa attatgaaag cagaattttt gacaccagtt gttacaatat ttgatgaaga
    12181 aggaaattta gataaagaag gtaataaaaa agtgtatgac catcttatag atggaggtgt
    12241 agatggacta gtcataatgg gaagtactgg agagttcttt aatatgtcta tggatatgca
    12301 aaaagaactt atagatttag taactagtca tgtaaataag agagtaaaag tgtttatagg
    12361 tacaagtcgt atgtgtataa atgaatctat agaactttca aactatgcac ataatagtgg
    12421 agcagatggt gttatgataa taagccctta ttatttctct cttgatgatg atagtattga
    12481 gttattttat agtgaaatag ctaagaatac agaggctgaa atatatttgt acaattatcc
    12541 agatagaact ggttatgatt taagtccaga acttacatta agacttgcaa gaaaacataa
    12601 aaacatagtt ggatataagg atacagtaac tttgatggga cataccagag atttaataaa
    12661 tactatgaaa atagagttcc cagaatttaa ggtttatagt ggttatgatg aaaactttat
    12721 acataatcta atgtctgggg gagcaggttg tatagggggg ttatcaaatt taattccaga
    12781 agtttgctcc aaatgggctg aagcttacaa taatagagat atggagaagg ttatagaaat
    12841 tcaaaaatat attaataaag caatggattt ttacttaata accaatccat ttataccagc
    12901 aatgaaaaaa gctatgaata tcagagggtt acaaataagt gattatatga ctgctccatt
    12961 tgtaaaacca aatgaaaaac aagtatgtga aattaaaaaa ttgatgaatg aattaaatat
    13021 atgtcaactt actaagtaag atatatatga gcaaaaaaag agtgtttaaa ctgattttta
    13081 taatcattta aacactcttt ttatatatgt taaatcaact ttgaaaagtg gtcaacaata
    13141 aattacaatt atgttaataa aaattaaata atactaagat agcatattag tttaaaagtt
    13201 agcacaaata ttaaagcaat agcctttatc tattaaaaat aatcttggag ttttacaaac
    13261 atttttagat aagtataagt taacaataag agccacatat aaactactac aaatcatgta
    13321 ataattaatc taataaatga taaacactta gacttcaagc ttaaaagtat aaagataaat
    13381 gaatttacat atcaagattg gcaataattt tgtgatacaa agcactatag taagaatgat
    13441 ttaataccaa tgattttgaa agaacatata gaaaaataca attagaataa aataaatttt
    13501 agagggaagg cattgccttc cattttttgt gtttttttag aattgggtaa attttacgat
    13561 tctagtaaag cacaaaagaa aatctgttgc tcttgaacat tttatattag taattatagg
    13621 tagcaatata caattactac aaaagtgaaa tattaatgaa atgtaaatca atttaaaaaa
    13681 attaaattaa aaattacaat agatatataa gaaatttaaa ggagaaataa gacatgaaaa
    13741 atgtattaag tgttgaaaaa atagaaaa
//
LOCUS       DAELJO010000093        13629 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELJO010000093 DAELJO010000000
VERSION     DAELJO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.093"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            136..1587
                     /locus_tag="KPB88_002275"
     CDS             136..1587
                     /locus_tag="KPB88_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0054547.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1806..2267)
                     /locus_tag="KPB88_002276"
     CDS             complement(1806..2267)
                     /locus_tag="KPB88_002276"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG0054548.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2372..3565)
                     /locus_tag="KPB88_002277"
     CDS             complement(2372..3565)
                     /locus_tag="KPB88_002277"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG0054549.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGTDTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDIYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3588..4253)
                     /locus_tag="KPB88_002278"
     CDS             complement(3588..4253)
                     /locus_tag="KPB88_002278"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG0054550.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4287..5399)
                     /gene="ribD"
                     /locus_tag="KPB88_002279"
     CDS             complement(4287..5399)
                     /gene="ribD"
                     /locus_tag="KPB88_002279"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG0054551.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5531..5652)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5841..8324
                     /gene="recQ"
                     /locus_tag="KPB88_002280"
     CDS             5841..8324
                     /gene="recQ"
                     /locus_tag="KPB88_002280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG0054552.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNINSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILTNKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8413..8524
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8666..9967
                     /gene="thiC"
                     /locus_tag="KPB88_002281"
     CDS             8666..9967
                     /gene="thiC"
                     /locus_tag="KPB88_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0054553.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            10021..10215
                     /gene="thiS"
                     /locus_tag="KPB88_002282"
     CDS             10021..10215
                     /gene="thiS"
                     /locus_tag="KPB88_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG0054554.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10208..11011
                     /gene="thiF"
                     /locus_tag="KPB88_002283"
     CDS             10208..11011
                     /gene="thiF"
                     /locus_tag="KPB88_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG0054555.1"
                     /translation="MVKIKIFVNELLTDVEENTTVYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11074..11844
                     /locus_tag="KPB88_002284"
     CDS             11074..11844
                     /locus_tag="KPB88_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG0054556.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARVSRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11861..12964
                     /gene="thiH"
                     /locus_tag="KPB88_002285"
     CDS             11861..12964
                     /gene="thiH"
                     /locus_tag="KPB88_002285"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG0054557.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12965..>13629
                     /locus_tag="KPB88_002286"
     CDS             12965..>13629
                     /locus_tag="KPB88_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0054558.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTIS"
BASE COUNT         5115 a         1612 c         2294 g         4608 t
ORIGIN      
        1 attcatttta aaccttgtaa tttgatttta taaatattat aattaaatta atgaattttt
       61 aacgaggaat tattcatttt tttgatttta agatttattg agaaaacaaa actaaatagg
      121 ggggtaatta atataatgga ttttttcaat ggtattttaa cgagtattaa caatgcagta
      181 gtatttgtaa acaattactt atggtcgtat atcttaatta taatgttagt tgtaattggt
      241 atttacttta ctattaaaac aaattttgta caattcagat attttataga aatgtttaga
      301 cttctgggtg atggtacagc gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt
      361 caagcatttt gtataagtac atcttcaaga gttggaactg gaaatatagc tggaatagca
      421 attgcagtgg tagcaggagg acctggtgct gtattttgga tgtggcttat agcattaatt
      481 ggttctgcat caagctttgt tgagagtaca ttagctcaga tttacaaagt gaaagatggt
      541 caagctttta gaggtggacc agcgtattat atggaacaag gtctaaataa aaaatggatg
      601 ggtattttat tctcaatact tataactata tgttatggtt ttgtatttaa tgcagttcaa
      661 gcaaatactg tttcacttgc atttaataat gcatttggaa taagtaaaat ggctatggga
      721 ataggtcttg gaattttaac tgcaatagtt atttttggtg gagtgcatag agttgcaaaa
      781 gtttctgaaa taatagttcc tgtatttgca ggattatata tattagttgc tttggctata
      841 gttgtaatga atataacaga gataccaagt gttatagcat tgatagttga aagtgcattt
      901 gattttaaag gaatggcgat tggtacattt atgggagttg taatgactgg agtgaagaga
      961 ggtctatttt ctaatgaagc aggtatgggt agtgctccta atgctgctgc aacagcacat
     1021 gtaactcatc ctgtaaaaca aggtttaatt caatctttag gtgtttttac agatactata
     1081 ataatatgta gttgtacagc attcatggta ttattatatt caggttatag tagtagtgga
     1141 gcaactggaa ttgaattagc tcaagaagct ttaactcatc atataggacc agttggaaat
     1201 atatttatag cagtttgtat attcttgttt gcatttagtt ctatagttgg taattactat
     1261 tatggtgaat caaatatgga gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga
     1321 gtttttgttg taggtatggt tttatttggt tcacttactc aagtagatgt agtttggaat
     1381 cttgcagact tatttatggc attgatggcg ataataaact taatagcaat agcactttta
     1441 ggaaaatatg catttattgc tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa
     1501 gaccctgtgt ttattgcaga tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg
     1561 gaattatctg aggaacattt aggataaaaa atagtataaa attataaaac cattttgaaa
     1621 gactagagta tgtatatact ttatatgaat ataatattga attaatttat attactggaa
     1681 tttttaattc cagtaatatt tttttattat ataatttagc tttattaaaa gtttttatta
     1741 ttaaaaatag cttatttatc gtaaagtgaa tgatattggg ttttaattat tatgaatgtt
     1801 agtatttaat ttaaagactt aaataaattt gccatttcta gcgcattcat agcacaatca
     1861 taacctttat ttccagcttt tgtacctgct ctttcaatag cttgttcaat attttctgta
     1921 gttacgattc caaatataac aggtaattca ctgtctaaag atactttggc tatcccttta
     1981 gagacttcac tacatacata atcataatga gaagtagccc ctcttataac acagcctaaa
     2041 cagattatag catcatactt accactttta gccatttttt gagcaacaag aggaatctca
     2101 aaagctccag gtacccaaac tatttctata ttttctggag aaacgtcatg tcttaaaaga
     2161 cagtcttcag ctcctgataa aagttttgat gttataaatt cattaaaacg agaatttatt
     2221 atacctattt ttaaatcttt tccaataagt tttccttcat atatcatttt tatttcctcc
     2281 aattttattt atgttataga tttatattta taaatatctt taatttaatt ataaaaaact
     2341 acttagtaga aaaatatcgt catataatca tttatataat atctaaaaga tgtcccattt
     2401 tatctttttt agtttttaaa tatagcttat taaagtcatt aatttcttca ttaataggga
     2461 ttctattttc gatttttatt ccatatttct ctaattgatt tattttatct gggttattac
     2521 ttaaaagatt tatacttttt atgtttaaat tttttaatat ttgagcagcc atatagaaat
     2581 ctctcatatc ttcttcaaat ccaagcatta aatttgcttc aacagtatcg taaccttctt
     2641 cttgaagttt ataagcctta attttattta aaagaccaat acctctacct tcttgcttca
     2701 tataaattaa aactccactt ccattttcaa caattttctt catagaagat tctagctgta
     2761 atccacaatc acattttaga gagtggaaca catctcctgt taaacattca gaatgaagtc
     2821 taactagagt attttctata tttatatttc cataagtaag ggcaatgtgt tcttcatttg
     2881 aataaatatc tctatatcct atgatttcaa aatttccata ttttgtcgga aggaaagcac
     2941 tagatacttt ttctatagta gtttcattta ttttgcggta ttctataagg tcttttatag
     3001 tgatgatttt taaattatgc ttttctttga aaatcattaa atcatcaact cttgccattg
     3061 taccatcatc tttcattatt tcacagataa gaccaatctc tttaaaacct gcaagtctag
     3121 caaggtctac tgaggcttct gtatgtccat ccctgacaag aaccccacca gtttttgcta
     3181 ttaaaggaaa gatgtgacca ggcgttttga aatctttact tgtagaattt tcatcacaga
     3241 acaattttac agtttcagct ctttcatatg cagatatacc agttagagta tctgttccat
     3301 ctatagtaac agtaaaggct gtttgaaatg tatctgtatt ttctctaacc ataagaggta
     3361 tattaagact tttaaatttt ttttcagtgg caggtagaca aattagacct ctaccatatg
     3421 tagccataaa atttattgat tcaggagtag ccatttcagc tgccatcaat aaatctcctt
     3481 cattctctct actttcatca tcaactacga ttaccatttt cccatttttt atatcttcaa
     3541 tagcttcttc aatagtatta aacatgtaat tcactccttt attattttta aaaaccattt
     3601 ttaaataaaa aatctttatc tatatttgat ttatggttac tactattaga atttaattcc
     3661 ttgtagttat tagccataaa attattaaca tactttccaa ttacatcgct ttctaaatta
     3721 acgaaatctc caacattctt tgtcaagaga atagtgttaa ttttagtatg aggtataata
     3781 gaaaccttaa atatttcatc atctaaatat gatatagtta aacttacacc atcaatagca
     3841 acagaacctt tgagtatcat tgaggataaa agttttttat caggtcgtat acttactaaa
     3901 actgcatttt catctttttc aaatttgcag atagttcctt taccatcaac atgacctgtt
     3961 actatatgac caccaaatct tgaagaaaga ctcatagctc tttcaagatt taccttgtca
     4021 tttgcattta ataaacctaa atttgtggaa cgaattgtct ccatcataac atcggctgta
     4081 aattcatttt ttccgaggtt tgaaacagtt agacaaactc catttacggc tatactatct
     4141 ccaagatttg tgccatctaa tattttattt gatagtatgg ttacttttcc actcttacca
     4201 ttagcagtaa tttttctaag tattccaact tcttctacta tgccagtaaa catcctaacc
     4261 tcctatatga atgtattaac aaaaatttaa tttaaaacat ctgattctat aagaatatcg
     4321 ccatctatca aagttacatt attgattgtt aactgtgttg aatctgacaa atgatttatt
     4381 ccttctccag aaacgaatgt aggtgcatct ttaccaccta taatcttggg tgatagaaat
     4441 aatttgactt tatcaactaa cttatttcta aagagactgt cattgagagt agcaccacct
     4501 tcaactagaa cagaatcaat gtttaactct cctaatttat acattacaaa ctccaaatct
     4561 actaagttgt tttttgaagg agaaattatt gtttcaacat tattttcttt taacatagat
     4621 aattttatac tttctttgca acatgtgaaa actatagttt tactggtttt atctttaacc
     4681 aaattactat taagtggcaa ctttaggtgt gtatctatta ctattcttgt aggatgagac
     4741 actttttctt gattgagcct gcaagttagt aaaggattat cgttcaaaac tgtatttatg
     4801 ccaaccatta tactttggta tttatttctg tatttatgag ttagatacct tgatgattca
     4861 ttagatatcc attttgattc tagtgatttt gtagcaattt taccatctag acttactgct
     4921 gattttacaa tacaaagagg tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa
     4981 tctcttgctt catcttctaa tattcccaca gtaacatcaa tattgttatc ttttaatttt
     5041 tttactccat taccacaaac aagtggattt gggtctaatg tagatattac aactctctta
     5101 atcttatttt gtataatttt atctacacaa ggaggcgttt tcccataatg agaacatggt
     5161 tctaagttta cataaagagt agagtctttt agactttgtt tagcactgtt tatagcattt
     5221 acttcagcat gattagagcc aaatttctca tgatatcctt tgcctattat attactatct
     5281 ttaacaatta cacacccaac taatggattt ggatttacaa agccttctcc atttttagcc
     5341 aattcaatag cttttttcat ataataaata tctttttctt tttgattcac aaaattcaca
     5401 atatgtacac ctcctattta attggataat ttttaaacaa aaaaccctga gaattaactc
     5461 agggtttaga ataatctaca ataaatgttt aataaatgta gaacaaaatt gctctacaaa
     5521 aacactcatt aaatatcttc ttccatccag actttactgt cggctttgga atttaaccaa
     5581 atcaaccaag ttttaccttg gctcgcgggc tataccgccg attgggaatt tcaccctacc
     5641 ccgaagatta agtattaaat ttatatttag aatgtatcat aaattatttt actttgcaaa
     5701 ctattttttt aaaaaatata tgttaatata taatatagaa tgtctataaa ctttgaaata
     5761 ccacgattgt gaatataaaa ataaaaattt gaacataatc aggaaagaag tctaattaat
     5821 ttaaattagg agagtaatat atgaataaaa aacttttaga aatactatca aaatactatg
     5881 gatatacaag ttttagaaaa ggtcaggaat ctataataaa ttctatctta agcgaaaaag
     5941 atgtgttagc tattatgcca acaggagggg gaaaatcaat atgttatcaa attccagctt
     6001 tgattttaga tggaatgaca atagtaattt ctccacttat atcacttatg aaagaccaag
     6061 tagatgcctt aaagactatg ggaattagtg ctgcttttat aaatagttca ttaagtagca
     6121 aagaatttaa tgaaatatta aataatatta gaaaaaataa ttataaaata ctttatattg
     6181 ctccagagag attagatgca caagagttta ttgagctaat aaataataac aatgtaagtc
     6241 aagttgcgat agatgaagca cattgtgtat ctcaatgggg gcatgatttt aggttgagtt
     6301 atagaaggat ttctgatttt ataaaaaatt tgcctaaaag acctatagtt acagcattta
     6361 ctgcaacagc ttcagaagag gtaagaactg atataataaa tttactgtgt ttagaaaatc
     6421 cagattatta tataactgga tttgatagag agaatttatc aataaatata gtaaaatcat
     6481 ctagtaaaaa taagtatatt ttagattata ttcaaaatca taaaaatgag tctggaatta
     6541 tatatgttgc aactagaaag gaagttgaaa atatatataa tggtttatct aagagaaata
     6601 tatctgtgtc aaaatatcat gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag
     6661 attttataaa tgataatgtg gatataatgg tagcaacaaa tgcatttgga atgggtattg
     6721 ataaacctaa tataagatgg gttatacatt acaatatgcc tcaaagtata gaaaattact
     6781 atcaagaaat aggtagagct ggtagggatg gagaaaaaag tgagtgtgta cttttatttt
     6841 ctccaggaga tgtacatata caaaaatatt taattgatat tggagttgaa aatcctgaaa
     6901 gaaaattatt tcagcataag aagcttcaat acatgattga cttagtttac agtaattcat
     6961 gctatagaaa gacaatattg agttattttg gagaaaatta tatagacaat tgcaataatt
     7021 gtagcaattg tttagtagag ggtgaaatag tagataaaac tatagatgca caaaaagtca
     7081 tttcatgtgt agctagaatg aaaagaagtt atggtgttac tactattgta gatgtgctta
     7141 gaggttctaa gaataaaaag attttacagc ttggattaaa tactttgtct acatataaca
     7201 ttatgagaga ttattctagt gaagacttaa agaattttat aaatacattg gtttctcatg
     7261 gttttttaga tttagttgag actctaggca atggaaatag aggtagtttt cctactataa
     7321 gattaaatga gatgtctatg aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa
     7381 ttgttatgac aaaatcattg gaagtagagg atgagttata taatgcttta agagaactta
     7441 gacattcaat tgcaagtgaa gaaaggatag ctccatatat ggtatttgga gatggtacac
     7501 ttagagccat gtctagtagt tatccaatta ataaagaaga aatgctagat atctctggag
     7561 taggagaaat taaatatcaa aaatatggta gagattttga agcagtaatt aaagaatatg
     7621 tcgaaaaaaa taatataaat aaaaaaatag atgaaaaaaa tgtcgattca aagaacatta
     7681 atagtgattt cttagaggtt aatacagata aagttttata tgaaaaactg ttagtcatta
     7741 gagaagaata tgcaacaaag gaaaaattac ctgcatatat gattttaacc aataaggctt
     7801 tgaaagaaat aagtggaaga tatccacttg atgaagaaca attaaaagat atttctggca
     7861 taggtggagt aaaatttgaa aaatatggag ctagtatttt ggcagaggtt aataaatatg
     7921 taaaagaaaa taatattaat gttatttggg agaaaaaggg caaaaggaag ttaatattag
     7981 atggagaaag tagaaaaaat aatgaaatag cattagattt gctaaatcaa ggaaaagata
     8041 tacaaagtat cagtcaagaa atagaagttt cactgtcaac cattattgga tatgtgcatg
     8101 actatgtaaa ggatggacat agtatatcat ttgaattagg actagaaaat ttttataatg
     8161 aagataaaaa gaagatgatt ttagaagttt gcgaaaaagt tggctatgat aatttaaata
     8221 acataaaaag aaaacttcca gattcaataa agtatgaaaa tataagagct gtgatacttg
     8281 actcttatct tgaaaactta aaaaataata ttatagcaga gtaattttta acttaataaa
     8341 aaataaaatt tgtattattg tgttgaacta ataattttgc tgtgttatac tgaatttgta
     8401 aaaataatga aaaattagag ctaggggcgc tagaattggc tagctgagag ataaagccgt
     8461 aacttacaaa ccctaataac ctgatcaaga taatgcttgc gaagggaagc tttttaatat
     8521 aattttatta tataaatagg caaattattc aatgttagga gaaatttgtc taagtgtata
     8581 tacgattata agagtgcttt ccaaacgagg aaaagcactt tttttatttt caaaaactat
     8641 aactttaatc ttggaggatt gtaatatgaa ctatacaaca caaatggatg ctgctagaaa
     8701 aggcataata actaaagaaa tggaaatcgt ttctcaaaaa gaacaggtag atgtaaatga
     8761 attaagagaa ttaatagcaa atggacaagt tgttatacca gctaataaaa atcataaatc
     8821 tttaagtgca gaaggtgtag gtaaaaattt aagaacaaaa ataaatgtaa atctaggaat
     8881 ttcaagagat tgtaaggata tagaaaaaga acttgaaaaa gttagagtag caatagacat
     8941 gaaagctgaa gctataatgg atttaagtaa ttatggcaaa actagagaat ttagagaaaa
     9001 ggttgttgaa atgtcacctg caatgatagg ttcagtacct atgtatgatg cagtaggata
     9061 tttggaaaaa gaattaaaag atataacaga agaagagttt ttaaatgtaa taagacaaca
     9121 tgctattgat ggagtagact ttattactat acatgcaggt cttacaagaa gtgtttgcca
     9181 aaaaataaaa aatcatgaga gattgactca catagtatct cgtggaggtt ctttattgtt
     9241 tgcatggatg gaattaaata ataaggaaaa tcctatttat actaattttg ataaaatatt
     9301 ggatatatgt gaagaatatg atgttacatt gagtttagga gatgcttgta gaccaggttg
     9361 tataaaagat tctactgatg gtgttcaaat tcaagaactt gtagtattag gtgaacttac
     9421 taaaagagca tgggaaagaa atgtacaagt aatgatagaa ggtcctggac acatggctat
     9481 tgatgaaata gaagcaaatg tagtattaga aaagagatta tgccatggag caccttttta
     9541 tgttcttgga ccattggtta cagatatagc accaggttat gaccatataa caagtgcaat
     9601 aggaggagct ttagcatgtg ctaaaggagt agatttttta tgttatgtaa ctccagcaga
     9661 acatttaaga ttgcctaatt tagatgatat gaaagagggt ataatagctg caaagatagc
     9721 tgctcatgca ggagatatag ctaaaaatgt taaaggtgct cgtgaatggg ataataagat
     9781 gagtaaagct agagctgatt tagactggtg tgagatgttt agacttgcaa tagaccctga
     9841 aaaagctaag agatacagag atgaatcaac tcctactcat gaggatagtt gtactatgtg
     9901 tggaaaaatg tgctcaatga gaacagttaa gaaaatatta aacaatgagg aacttaatct
     9961 aatataattt ttggctagta tgactggaat aaaaataagc tgaaaagagg aggtgattta
    10021 gtgaaggtta atgggaagga aattgaattt gaaaaagatt tgactgttat agatttatta
    10081 aataaatata atttaaaaag tgatagagtt gtagttgaag tgaatttaga gataattgaa
    10141 gaatctaact acaatacata tgtattaaaa gacgaggaca ttgtagagct tattagcttt
    10201 attggaggtg gttaaaataa aaatatttgt aaatgaatta ttaacagatg ttgaggaaaa
    10261 tacaactgtt tataaagtta gagatagcta taacaataaa tgtgatgtat tggtgttaaa
    10321 tggatatcca attaaaacag atatgcccct ttgtgaaaat gataaattaa cacttataca
    10381 aaaaggagtt aagccatcac ttgatgaatt ggaaagcttg ttgattgcta gacatactcc
    10441 aaatatacat aataaattaa aaaaagggaa agttgctata ttgggattgg ggggacttgg
    10501 ttccaatata gctatctctt tagctagaat aggagtggga gaacttttac ttatagatta
    10561 tgatgttgta gaaccatcta atttaaatag acagcagtat tttatagatg atataggaaa
    10621 actaaaaact gatgccatga tagaaaatat aaaaaaaata aatccattta taaaattaaa
    10681 taaacgagat atttttttaa acaagaacaa tatggatact attaaggatt cagatttaat
    10741 aattgaggcc tttgatgatg cttcatgtaa agcacaggta tgtaattatg tattaataaa
    10801 tttaaaagat aaatatttaa tagcatcatc tggtatggca ggttactatg attcaaatat
    10861 aataactact aagaagataa aagacaggtt ttatatatgt ggggattttg tgaatgaagc
    10921 taaatttggt gaagggctta tggctccaag ggttgccatc tgcgcaaatc atatggcaaa
    10981 tttggcaacg caaatactta tagatatgta aatattaaaa ttagatataa aaaagattga
    11041 atattaaaaa aaattaacgt ggagggaaat aatatggata aattgatact tggtggacat
    11101 gaatttaata gcagattatt ggttggaaca ggtaaatatg gttcaaataa tatattgcct
    11161 gaggtaataa aagaaagtgg aagtgaaata ataacaatgg cacttagaag agtggattta
    11221 gacaataaac aagaaaacat attaacgtat attccaaaag agatgacaat acttccaaat
    11281 acatcaggag ctactaatgc agaagaagct gtaagaatag ctagagtatc tagaaaaatg
    11341 ggatgtggag attttataaa aatagaagta atatcagata ctagatattt acttcctgat
    11401 aatgaagaaa ctataaaagc tacaaagata cttgctgatg aggggtttat agttcttcca
    11461 tatatgacac cagaccttta tgctggaaga agacttatag aagctaatgc agcggcagtt
    11521 atgccacttg gagctccaat tggttcaaat agaggtcttc aaatgaaaga aatgataaga
    11581 ataatgatag atgagttgga tataccaata atagtagatg caggtattgg aaaaccatct
    11641 caagctatgg aggctatgga aatgggagct gatgcagtac ttgtcaacac tgctatagct
    11701 tcagcaggag accctgtaca aatggctaga gcatttaaat tagctgtaga aggtggaaga
    11761 gaagcatata ttgcaaaaac aggaaatgtg tcagaatttg caaatgcatc ttctccttta
    11821 acaggttttt taggcaattt atagaaagtg gtgattggtg atgagttttt atgatgtaat
    11881 agaaaaatat agggattttg attttgatgg atatttaaat aatgttactg ataatgatgt
    11941 tttaagaagt ttatcaaaag acaagcttga agattttgat atattaaatt tgctttctaa
    12001 aactgcagta aaacatttag aagatatggc acaaaaagca cataaattaa gtgttcaata
    12061 ctttggtaaa acagtatgtt tatatacacc aatgtatata gcaaattatt gtgttaatca
    12121 atgtgtttat tgtagctata atataaagag tggaataaaa agaaaaaaat taactatgga
    12181 tgaaattaga gaagagggag aagcaatttc aaaagaaggt ttcaaacatc tacttgtttt
    12241 gactggagaa agtagttttc actcaagtgt agaatatata ggtgaggcta tagaaatatt
    12301 aagagagaaa tttccctcta taggtataga agtttatcca atggaagtag aagaatataa
    12361 gtacattgta gataaaggtg ttgaaggatt aacagtatat caagaaactt atgacgagga
    12421 aatatataag agagtacata taaaaggccc aaaatcaaat tataaattta gactagatgc
    12481 tcctgaaaga ggggcaaaag caggtatgag gactttatca ataggtgcac ttttagggct
    12541 aaatgatttt agaaaagaaa ctttttttac aatattgcat gggaaatact taaaaacgaa
    12601 atatcctcat attgagcttt catattcaac accaagaatg agacctttta aagggtgttt
    12661 tgaggaattg gtagatataa gtgatactga tttagttcaa gctatggtat gtatgagatt
    12721 atttgaccct catgcagcta taaacatatc cactagagaa aatttagaaa tgagaagtca
    12781 tataatacct ttaggagtaa ctaaattgag tgcaggtgta tctactgatg taggaggtca
    12841 ttctcaagat gaacatgata ctgctcaatt taagataaat gatgaaagta ctgttaaaga
    12901 tgtggagaaa atgctaaact caattggtta tcagcatgtg tttaaagatt gggaaagatt
    12961 ttaaatgtat ctgataacaa acagaaaatt atgtagtgag gaacgatatt tagaagttat
    13021 taaagagtct atattgagtg gtgtagaaaa tataataata agagaaaagg atttagaata
    13081 tcaagagcta agaaaattat atatgaaaat aaaaactaaa atcaattgta tagattttca
    13141 agagcaaata tcagatgaaa gtttgaaaac taatataaat caaaaagagt gtagaaataa
    13201 attcaaggtt aattttataa taaatagtaa cattgaattt tttgaaaaag tggattgtca
    13261 aggtatccat cttcctttta aattattttt gaatttaatt gaaaataagt ataattttaa
    13321 tgaaaataaa atactaggat tatctttaca taaagtagag gaagtagatt atttagaaaa
    13381 attaataaga aatcaaaata taaaaataga ttacataaca ttatcacata tatatgaaac
    13441 taaatgtaaa gaaggattaa atccaaaagg aattgagctt ttaaaagaag ctaaaaagat
    13501 tactgatatt aaaataattg cacttggggg aattttacca tctaatgtca aagaaacatt
    13561 aaaatactgt gatgattttg ctataatgtc aactataatg aggtctaaag atattaaaaa
    13621 aaccatatc
//
LOCUS       DAELJO010000094        13445 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELJO010000094 DAELJO010000000
VERSION     DAELJO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.094"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            204..1532
                     /locus_tag="KPB88_002287"
     CDS             204..1532
                     /locus_tag="KPB88_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0054559.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
     gene            2285..3118
                     /locus_tag="KPB88_002288"
     CDS             2285..3118
                     /locus_tag="KPB88_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0054560.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3211..4143
                     /locus_tag="KPB88_002289"
     CDS             3211..4143
                     /locus_tag="KPB88_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0054561.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4195..4677
                     /locus_tag="KPB88_002290"
     CDS             4195..4677
                     /locus_tag="KPB88_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0054562.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4689..5558
                     /locus_tag="KPB88_002291"
     CDS             4689..5558
                     /locus_tag="KPB88_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0054563.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5601..6356
                     /locus_tag="KPB88_002292"
     CDS             5601..6356
                     /locus_tag="KPB88_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0054564.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            6398..7219
                     /locus_tag="KPB88_002293"
     CDS             6398..7219
                     /locus_tag="KPB88_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG0054565.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7224..7985
                     /locus_tag="KPB88_002294"
     CDS             7224..7985
                     /locus_tag="KPB88_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG0054566.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIFVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8146..8403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8516..8755
                     /locus_tag="KPB88_002295"
     CDS             8516..8755
                     /locus_tag="KPB88_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0054567.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            9059..10054
                     /gene="ilvC"
                     /locus_tag="KPB88_002296"
     CDS             9059..10054
                     /gene="ilvC"
                     /locus_tag="KPB88_002296"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG0054568.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            10107..11795
                     /gene="ilvB"
                     /locus_tag="KPB88_002297"
     CDS             10107..11795
                     /gene="ilvB"
                     /locus_tag="KPB88_002297"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG0054569.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVASMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(12156..13286)
                     /locus_tag="KPB88_002298"
     CDS             complement(12156..13286)
                     /locus_tag="KPB88_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0054570.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPSILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT         5358 a         1368 c         2323 g         4396 t
ORIGIN      
        1 acttagaagt attattatat ttggattttt ggtttttata atttttttat atttaaacat
       61 aactaaaaat aaaagattac taaagctaag tagagtttat tttattttaa taaataataa
      121 agctattttt ctatatattg gcataatata tgcttattta actatgaaat taaaattaaa
      181 taggattaat gaggaggaaa aaaatgggaa gtaatgaact gaaaaagact cttggcgtat
      241 cagcagcatt atcaactgtt gttggttccg ttataggagc gggcgtattt tttaaaccac
      301 aagcagtcta tacattaact ggaggagctc caggtcttgg tatattggca tggttaatag
      361 ctggaatcat aactataaca gctggtctta cagctgcaga ggtatcagtt gcgataccaa
      421 aaactggtgg aatgatggta tatataaaag aaatttatgg agaaaaatta ggttttttaa
      481 ctggatggat gcaaattgta ttattttatc cagggatgat ggcagcatta ggtgttatat
      541 ttggagaaca agcatcagca ctaattggta gtccatcact tttacttcca atagcaattg
      601 gaattataat aatagttgca gggcttaata tgttaggttc aaaaactgga ggagtcatac
      661 aaactgtttc tacaatttgt aagttaatac cacttatttt aataatggtt gttggattta
      721 ttaaaggtgg tggaaataat ccaatactta cacctatggt tggtgaagga ttaagcctgg
      781 gtagtgtact aggtcaagtt ttaattgcta tattatttgc ttttgatgga tggatgaatg
      841 ttggtacttt agctggagaa atgaagaatc ctggaaaaga ccttccaaaa gctataatag
      901 gtggattgtc agttgttatg gctgtttatt ttataataaa tttagcgtat ctttgggtat
      961 taccagctaa tgaattagca aattatgctt ctcctgcatc agcagttgct gaagttatat
     1021 ttggttcaat gggtggaaaa ataatatctg taggtatatt aatttcagtg tttggagcat
     1081 taaatggatt tttactaact ggttcaaggg tggcatatac tttagctaca gataagactt
     1141 taccaaagta tggtatattt tctaaattaa attctgctca agttccagca aatgcaatag
     1201 cattagtatc tgttattgca tctatatatg ctttgtcagg tcaatttaat ttattaactg
     1261 acttagctgt atttgctact tggatattct atgtattaac ttttataggg gttatgaaac
     1321 ttagaaagac tcatccaaat ataccaagag agtataaagt tccattatat cctattgttc
     1381 ctataatagc tatagcaagt ggtatatttg ttgtagttaa tcaattgtgt tttgcaggta
     1441 tgaaaactac tatgatatct ataggaggtc ttgtaataac agctataggc ttaccagtgt
     1501 atgcttatat gactagagga gtaaaaagat aaaccaagta gtttacaaat gcttcataat
     1561 taaaataaaa gagactgtct caaatagaga taagtctctt ttttattgtt aaaatggtaa
     1621 agatatgttt aattttacaa aaaaagagcg ttccatgaac taaaaagttt attttaaggt
     1681 attcatttta tttttagttt tttgattata aaattaaaca tctaaaaaat tatttatttt
     1741 aaaatacttt attttaaagt gttttttatc aaactaagtg gtatctgtac aacaaatatt
     1801 atacatagct tttatgaaaa ttaaatcttt ctgttatata taatagaata ttgtattata
     1861 tagaaaatat tacctgatta atatagtgat ttataaatga gattagaaaa ttttatttga
     1921 taacaaaaat tagataatat gaatatataa tgtatgtatt aaaaatgtgg caaatagttg
     1981 ttttgctatt tattatttgt ttaatatttc taatttataa aaaaaaaatt ataaatttta
     2041 aaaattataa atgacatttt aactagaatt aattgtaaaa aacactatat aatactcaat
     2101 tttcaacatt ctatacaatt atacagaaat tatacagcat aataatcacc gaaataacaa
     2161 attttaaaaa ttaagcaaaa ctttttgtcg aagttcaacg tctatatata tgtagtgtaa
     2221 atttttggga taaaatacta caaaaatata aatgtaaaaa tatagtaata ttggaggtcg
     2281 aacgatgaga aataaaaaga aaaaaataaa tagaaagaag ttgtatttgc ttttaagtgc
     2341 tattgtagtg tgtctggctt ttatagtagt tggtgttcgc gtcagctctt taaatatgaa
     2401 ggaaaatgaa attagtagaa atgcaaaact gtcaagttct actattacag atatagtatc
     2461 taatacatca atagaagtta gtaaagggcc aagtaaaagt tctggaaaaa tagcatacat
     2521 aactatagat gatggtccat ctaaatttac tgacgagatg ataaaaactt taaataaata
     2581 caatgttaaa gcaacttttt ttatgataga tgggaatatg aaggaatatc cacagcaagt
     2641 taaaaatata attaaaaatg ggaacacagc aggttttcat agtgtatcac atgatattca
     2701 caagctttat gttactagta catcagcaaa agaggaattt gatacaaatg atcaaacttt
     2761 ttataagata actggtaaac attcaaaagt aataagaata ccttatggaa gcaaaccata
     2821 tacaccacaa gcctcttatc aggctttagt tgatgctgga tataaaattt gggactggga
     2881 cttagataca gaagactgga gatctaattc ttctcaaatt gtacaaaatg taaaaaatca
     2941 tattaagaat agaaaaggtg aagataaaga ccaacttgta gttttaatgc atgagaaaaa
     3001 acagagtgca gaagctttag attcagtttt aaaattctta tcagatgaag gttatgaatt
     3061 tgcgccagta gatcaaaacc aaataccaaa aaattattgg ttacgtaatt tagagtagtt
     3121 aaaatataag aatattgatt gatattttta gaaattgaga ataaaaatga gtgaggtgta
     3181 ggtatattga ataaaaaact ttatatagga atggtcggaa tattgtcttt gatgatggtt
     3241 ggttgtaata aaagtttagc aaaagtaaat gatgtggaaa tcacaaaaga acagtacaaa
     3301 aaaacaaaag cagttttatc tgctactaat aattatataa atgggcagtc tttagatgaa
     3361 ttagaaaaaa ctttggataa aaaaggtaga aataaattag aaaatgttat aatatcgttt
     3421 atggtggata atgaactttt atatcaagag gctaaggata aaggactaac tccaagtaaa
     3481 agtgaagtag actcaaaata tcaagaacta gaagataaga tgaatttgaa tacaagctat
     3541 aaggaaaaaa tggacaaagc aggtgttgat aaagattatt taaaacaaga aatatctaga
     3601 gatttagcaa tagataaaaa taaaaaggcc tttgaagata gaataaacat aagtgataat
     3661 gatatggaag cctactatac aagtcataaa aaagatttta atgtagaaga agtaagtgct
     3721 tctcagatac ttatatcgac tttagataaa aataaaaaag aagtaagcaa agataaaaaa
     3781 gaagctttga agaagaaggc agataatata ttgactaaaa tcaaaaatgg agaaagtttt
     3841 gaaagtttgg ctaaaaaata ttcggatgat aaagcaactg gaaaaaatgg aggacagcta
     3901 ggatatttta caaaggatga taaaaatgct gagtttacaa aggaagtgtt caaattaaag
     3961 aaaaatgaag tttcaaatgt gtttgaaact agttatggat atcatatagt caaagttact
     4021 gataaaagag aaagacaaaa aagttttaat gagtgtcaga gtttaataag agaatctatt
     4081 ttaaatgaaa agtatattga gcatataaaa aaacttaatg aagatgctaa aatagacaga
     4141 tagaatttct aacagctcaa tcctagcctg taatcggagg gagattttac aggaatgttt
     4201 aagagtttaa agagaacgaa agttgagaaa tacattatcg ataataaaga ctctttttat
     4261 agaatagcat atagttatac gaagaatgaa gaagatgcct tagatgtggt tcaagaagcc
     4321 atgtataagg cactatactc agtggaaaat attaaagaag taaattatat aaagacatgg
     4381 ttttataaaa ttttagttag aacgagtata gattttatta gaaaaaaccg taaatataat
     4441 aatatgacag atattgacct tatagatgag actggagagt atgataaata tactgatttg
     4501 gatttaagaa gagctttgga agaattacct attgaataca aatctattat aatattaaga
     4561 ttttttgaag atttaaagat tgaagaagta gcaataatat tggatgaaaa tgtgaatact
     4621 gttaaaacta ggttatatac tgctttaaaa aaactaaaat taaaaattga agagtaggga
     4681 taaaaagaat gacaagcaaa gaaaggttaa ataagttaaa agatgagtat cataaaattc
     4741 caatacccaa gaaattagat actataataa atcatgaaaa aatagaaaac atatatcgag
     4801 agaaaaataa aaaagtaaat agattaagat ttaaagttgc aatagccttt gcatgtatat
     4861 ttacagtatt agtaaatata agtcctgtat ttgcagacaa tttttctaag atacctgtaa
     4921 ttggagcaat agtagaagta ataactataa aaaattatag tttaaaaagt gaaaattatg
     4981 aagctgagat tgatattcct aaaatacgag gtttaaaaga taaaaactta gaacaaagat
     5041 taaacagtag ttttatggaa gatggaaaaa gactttatca tcaattccaa gagagaatgg
     5101 agaagataca atcatcaaag aataaaggat ataaatcttt aagtttgagt tatagtgtaa
     5161 aaaataattc taaaaaattt ctatcaatag agatgacaaa aaatgagata gaggcttcat
     5221 catatgtgag taaggtacat tatactattg ataaaaaaag acaaatagtt ttgactttgc
     5281 caatgctttt taaagatgat aagtatataa aagttataag tgataatata aaagaacaaa
     5341 tgagagaaca aatgaaaaag gatagtacaa aatcctattt tatagatcaa aaaaaagact
     5401 tgcctgttga agattttaaa acaattaata agtatcagga tttttatttt aataaaaatg
     5461 aagaccttgt aatttgtttt gatgaatatg aagtagcacc tggttatatg ggcgcagttg
     5521 agtttgtaat accatataaa gtaataaaag atttgtaaat tgaatagtag tttatatata
     5581 aatcttttaa ggaggacaat atgactacat ataaaaaact taaaaaatct atatttaaaa
     5641 ttgctatatt ttcaatatgc ttattagttt cattagtaga tacaaaccct atatttgcag
     5701 gtagttcctt taatgaaact tcaaatagtg atacgataaa ggtgttaaat gctaagagta
     5761 tcaactttaa acaagacaat tatgaagtga atgtgagtat tcctaaaata aaaggtttaa
     5821 aagataaaac tttagagaat aaattaaaca atgagctttt gcaagatggg aaaaaaattt
     5881 acagtgaatt tcaaaataaa atgaaaaagg aagataataa gggtcataga tacctaaatg
     5941 taatccataa tgtcaaaaat aattctaagg actttttatc aatagaaatg acaaaagaag
     6001 agatagaagc ttcctcatct ataactaaga agcattacac tgttgataaa aatagaggaa
     6061 tactattaaa tttaccaata ttttttaaag atagtagata tattagagct ataagtgata
     6121 atataaaaga gcagatgaga aatcaaatta agaaagatga tacaaaatct tattttatag
     6181 accaagataa agactcgcca gtgaaagatt ttgaatctat aaataagtat caagattttt
     6241 attttaataa aaatggaaat cttgtgatta gttttgatga atatgaagta gctcctggtt
     6301 atatgggagc agtagaattt gtgataccaa ataaagtaat aaaaagacta agatagatat
     6361 atagaagatt tttagataga ctggtaagga gaattatatg aatatatatg aacaagcaat
     6421 aagattaata gaaaataatg aggatttcgc atttgctaca ataacgtctc attcaggttc
     6481 aacaccacgt gaaactggag caatgatgat tgtgaaaaat gattctacta tatttggctc
     6541 agtaggagga ggtagtgtag aagcagaatg tataaaacat gcaattaatg ttataaaaaa
     6601 cagagaatca atgttgtata aatttactct aaacaaatct gatgttgcaa aactaggaat
     6661 gatatgtgga ggtactggag aaatacaaat agattttata gatagtaaat taaaaagtaa
     6721 tatagaaaaa tttaataaga gactaaagga aaatacaagt aaagcatata tatttggtgc
     6781 aggtcatatt tcaagagatg ttgctgtgat actatctctt ttagaattta gaacagtagt
     6841 aatagatgat agagaagaat ttgctaatca tgaaaggttt ccagaatcag aagtagtagt
     6901 tttagattct tttgaaaaca taccagattt tccaactgac gaaaatagtt atataattat
     6961 tttaacaaga ggtcatctat atgattcaag tgctttagaa tgggctctta aaagagaggc
     7021 tggttatata ggtatgattg gaagtagaac aaaaattgga ttgacttacg aaaaactcat
     7081 gaaaaaaggt tttaagaagg aagaattatc aaaagtacat gcaccaatag gcataaaatt
     7141 gaatgctcaa acaccagctg aaatagctgt atgtatagct gctgaactta taaattgtag
     7201 agctaataag gaaaaataaa ctaatgaaca cagggagtaa agatattgat actttaaagg
     7261 ttaattttag agggattgaa ataaaagcag ttttagtata taaaaataga aaaaatattt
     7321 ctataaaaat agatccattt ggcaaaataa ctgtaatgag tcctcctaaa atatccaaaa
     7381 aaataataaa agatataatt attgaaaaag gagactggat acttaaaaag tcagaaaaat
     7441 gtaaaggaag agaggaagta tataaacaaa gaatgtttat aactggcgaa aaatttcttt
     7501 atttaggtga agagtaccat ctagttataa aagaactgtt gagagattct gtcaaaaaaa
     7561 gtaactgtag aataactatt aacgaatatc aaatatttgt tcagacaaat gatacaagta
     7621 cagattttat taaaaaatct ttaaaatctt ggtataagat ggaaagtgaa agaatagtat
     7681 tagagagaat agattttctt aaattaaatt gtgaaataat gaagcaatta gttccagcga
     7741 gtgttaaggt aaaagaacaa aataagagat ggggaagttg tacagctcaa aaaaatattt
     7801 atataaactc aaaaatatct atggcaagat tagatgtaat tgattatgta ataatacatg
     7861 agtttagcca tttagtacat atgaatcact ctaaaaaatt ctatgattta gtaaaatcca
     7921 ttatgcccga ttataaagat aaagaaaatt ggctaaaaat aaatggatat aaaataataa
     7981 tctgaaaacg attattccaa aatataggcg taaagtataa aagaagtatt attgtgaaaa
     8041 aaataaataa tctgaaaatt ttaaaaaaag tcattgacat tgtaaatgat agggaatata
     8101 atctatatta acaacaaaac agaattaaaa tataaaatta aaaaattaaa agcaatgaca
     8161 gagacaacaa tactaatagt gttatccaca gagaaccaat gttgctgaga attggtgtta
     8221 acagtagtat aatgatcact ctggagcttt tgtactgaat atttttagta agtacaaacg
     8281 tatgtcaacg ttacatgact aaggttaagg taattaatta cccgacctga aaaaagaggg
     8341 gtagatgtat tttttatatc tacaaataag gtggtaccgc ggaatataac tttcgtcctt
     8401 ataagtaata tttatattgc ttataaagat gaaagttttt ttattttaag cgatataaat
     8461 aaatttaatc catataaaat taataaaaaa ataataaaaa taaggaggga tttttatgga
     8521 aaatactgta tatgctaaat taaaccaagg aatagattct tttttaagag tggctatgac
     8581 tctaagaaga agagaggttg atatacaatc aataagtatg actgtagata atatgaataa
     8641 ttcaggtata aaattaatag taaatgaaga aaagacatct ttagattctg tcttaaacca
     8701 tatgaaaaaa ctacatgata taagagagat aactgttgaa agaatgagac attaattaaa
     8761 tatattttat aaaactaaag ataaatatgc ttattttaaa ataaaaaact aaagataaat
     8821 attaaataag aaaaatctta gattaaaata taagtgtcat tgaatttatt agtttatcac
     8881 agttaataaa tttattagtt tgttgtagct aataaaaact gaaaattcta aaattataat
     8941 tgtaagatta tataaattta gaatttaaat ctgttgaata atattaaaac aatgataatg
     9001 agtatgaatt tctaataaaa ataaatatca taaaaataag aaaaagtatt gggaggaaat
     9061 gaaaatggca agaatgtatt atgaaaaaga tgtggattta gaggttttaa aaaataaaaa
     9121 ggttgcagtt ttaggttatg gaagtcaagg acatgcacat gcacaaaatc ttagagataa
     9181 cggtgtacat gtaatgatag gtctttatga cggaagtaaa tcagcacaaa aagcaaaaga
     9241 agatggattt gaagttaaga gtgtagcaga agctacaaaa gaaagtgatt tgacaatgat
     9301 gttaatgcct gatgaaaaac aaaagaaagt ttatgaagag agtgtaaagg ataacttaaa
     9361 agaaggtcag acattagctt ttgctcatgg attcaatata cattataatc aagtacaacc
     9421 accagaattt gttgatgttg taatggtagc tcctaaagga cctggtcact tagttagaaa
     9481 tgtatttaca aagggaagtg gagttcctgc actatttgca gtatatcaag actacactaa
     9541 aaaagctaca gagacagttt tagcatatgc aaaagggata ggagcaacaa gagcaggtgt
     9601 tttagaaact acttttaaag aagaaacaga aacagattta tttggagaac aatcagtact
     9661 ttgtggtgga ataagtgaac ttataaaatt gggatataaa acgcttgtag atgcaggtta
     9721 tcaaaaagaa gtagcatatt ttgaatgttt acatgaaatg aaactaatag tcgaccttat
     9781 atatgaaggt ggatttgaaa gaatgagata tagtataagt gacacagcag aatatggaga
     9841 ttatgtatct ggtaaaaggg taataactga tgctgcaaaa caaggtatgc aaaatgtttt
     9901 agaggatata caaaatggaa aatttgcaaa agcgtggatt aaagaaaatg aagaaggacg
     9961 tgaaaacttc ctaaaaacta gagaagaaga atataataca gaaattgccg aagttggtag
    10021 aaatctaaga agtatgatgt cattcttaaa ataaatatat attaaatgga ttagtttgag
    10081 ggaaagagtg gtgtatagta gtgcgtatga atggagcaaa ggtaatttta gaatgtttaa
    10141 agaaagaggg aattgacaca atatttggat atcctggtgg agctgtgata cctctttatg
    10201 atgctctata cgattattca gatgatttta aacatataag gacatcccat gaacaaggtt
    10261 tagttcatgc tgcagatggg tacgcaagaa gtactaatac agtaggagtt tgttttacaa
    10321 catctggtcc aggagcaaca aatgccataa ctggaatagc aacagctttt atggattctt
    10381 cacctatggt tgtaatttca gggcaggtgc caactagttt attaggaaaa gattcttttc
    10441 aagaaattga cataactgga gcaacattat ctatgacaaa acataattat ttagttagaa
    10501 acactaaaga attagttcct acaataaaag aagcttttag agttgcaaat tcaggtagaa
    10561 aaggacctgt acttgtagat gttccaaaag atttattttt agcagaaatg gattttagtg
    10621 gtgaggacta tgatttatgt caaatagatg attatatgga ttataagagt gattttgatc
    10681 tagatgatga aactaatatt aagcttttaa atgaagcaat agatatcata aaagaatcta
    10741 aaaagcctat aatatatgca ggtggaggtg ttaaatcttc agacagtgag gaaattcttg
    10801 aaaaatttgc aactaagata gatacacccg tactaaatac acttatgggg cttggaaata
    10861 tagatagaaa gaatgaatta tctcttggaa tggttggaat gcatggaagt agagaaagta
    10921 atttagcact ttctaattca gatttagtaa tagcgatagg tgcgagattt agtgatagag
    10981 tgataagtaa aagttctgaa tttgctaaaa atgcaaaaat aatacacata gacatagacc
    11041 catctgaaat aagtaaaaat atagaatcta atgtatcttt ggttggagat gtaaagctag
    11101 tccttagttt attaattgaa agggttgaaa gtaagaataa tagcaattgg aaagaagaga
    11161 taaagacatt tagaaagagt gagggtgtac aaacatatga atttcatcca caaaatatac
    11221 ttaaaaaaat aaatgaaaaa tatgaaacat taaagaaacc cactgtggta gtaactgatg
    11281 tcggtcaaca tcaaatgtgg gctgctaaat actggaattt taaaggtaat aaaagtttta
    11341 taacatcagc agggttagga actatgggat ttggtttagg tgcagctatt ggaactaaag
    11401 ttggtaatgt agataaaaat gtagttttag tcactggtga tggaagtttt agaatgaact
    11461 gtaatgaact agctacagtt gcaaattata atgtacctat gcttatttta ctactcaata
    11521 atagaacatt aggaatggta aggcaatggc aaaagttatt ttccaatcaa agatactctc
    11581 aaactgacat taatgaaaat gtggattatg ttaaactcgt aaatgcatat aatattgatg
    11641 gatataaggt agccagtatg gaagaattag gaaaggcgct agatatgata gattttaata
    11701 aacctgtatt tttgcaatgt gatatagata aggattatga tgtttatcct attgtagcac
    11761 ctaatgatgc attggaaaat ttgatatgta actaaaactt atactgaata ttgataaaat
    11821 tttttattaa atttttatta gtattcagtt tttgttgttt agagataaat tggcattttt
    11881 tatttttgca aataaatcaa tgactatata attaagaact tattaaattt taattataca
    11941 atttagaata gtattacaac ttaagtaaat attaagtttt atttgttaga tttattatta
    12001 ataccttata ttagttatgg ttgttatata ctctaaagcc ttgaaattac aagtatatag
    12061 atgattatat aaatttgata attttagctt ttaaaataga taaattagat tttgccaccc
    12121 atttgccacc gtattatatt tttgggtggc aaactctaat ttgtaatttt ttcaaatata
    12181 tctacagttt catttttcat tttatcagtt acatgtgaat aggtatccat tgtagttgat
    12241 aattggctat gacccaaacg gttttgtatg tctttaatgt tagcaccatt ttctaataat
    12301 agagtagcat gtgcatgtct taaagaatga aaatggaagt cattgtttaa agctactcga
    12361 atttgtctta ctatagtgtc taaagtgtga gtattcactt gttgaccatt ttctttggta
    12421 catacccaat cactatcaaa gtaaaattct ccgtatttta atttcatttt cttttgatat
    12481 aatttatgtt cttttaatgc ctttattaaa gtgtcacctg taaatatagt tctgcaagag
    12541 ctttctgttt ttggttgccc taattcaaac attccatttg gttttttaat caaagtatgc
    12601 tttactgtga tagttttatt atcaaggtct atattatccc attttagtgc aataatttca
    12661 cctcttcgca taccagtatg aaatccaatt agtaaaacta tacgttgaaa tgaatcttga
    12721 ggaaatatat ttagtatttg attaaattct tctaatgtaa tagttttaac tttattagtt
    12781 tctgttttag atttagtttt tggtatgctt acatattgca tagggttttc tcgtatgtgt
    12841 ttgtaaggat ggactgctga ttttaatgac ctatgtaata tggcttttaa tacttgtaat
    12901 gtattttgag agtaatcctc tttgtacttt ttatttatga agttttgtag tattgaagga
    12961 gttaaagctt ttactttgta agctcctagt tttggcttta tatgtttttc tatgtttatt
    13021 cggtagcttt cttgagtgtt gtatttacag ttaagtaaga catattcttt gtaccaaaaa
    13081 tctaagtagt ctgataaact gatgttgctt tcttcaaata ctatgccaga gttttcatat
    13141 tcatttagtg cttctcttaa ggctttttcg gcttcttttt tagtattgcc tccaactctt
    13201 tctacttttt ttctttttcc ttctactatg cctaggtcaa agtagtaata ccatttgtta
    13261 cttctttttc ttactccacc tttcataata gtatccctcc cttttagaat gtatgtttgt
    13321 ttggtgttta tataaaagag cagattaact gctctttata tacttttgtt atgtacaata
    13381 ttatcttaat tttgtttatc tggtttttac ttaattattt tcttaatatt cttacaattt
    13441 tttag
//
LOCUS       DAELJO010000095        13410 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELJO010000095 DAELJO010000000
VERSION     DAELJO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.095"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(118..369)
                     /locus_tag="KPB88_002299"
     CDS             complement(118..369)
                     /locus_tag="KPB88_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054571.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            458..1189
                     /locus_tag="KPB88_002300"
     CDS             458..1189
                     /locus_tag="KPB88_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG0054572.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFNKLNDVYKSLPAGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKVLDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYKELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSERLSFFDELMTLDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLI"
     gene            1248..2159
                     /locus_tag="KPB88_002301"
     CDS             1248..2159
                     /locus_tag="KPB88_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0054573.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2210..3022
                     /locus_tag="KPB88_002302"
     CDS             2210..3022
                     /locus_tag="KPB88_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG0054574.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3132..3824
                     /locus_tag="KPB88_002303"
     CDS             3132..3824
                     /locus_tag="KPB88_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0054575.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3911..4852
                     /locus_tag="KPB88_002304"
     CDS             3911..4852
                     /locus_tag="KPB88_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054576.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNNIKEKNNSDSNVSVNNKSEKQEDTTTENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNNIN"
     gene            4889..6046
                     /locus_tag="KPB88_002305"
     CDS             4889..6046
                     /locus_tag="KPB88_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0054577.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTSVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6198..6758
                     /locus_tag="KPB88_002306"
     CDS             6198..6758
                     /locus_tag="KPB88_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0054578.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6786..7277
                     /locus_tag="KPB88_002307"
     CDS             6786..7277
                     /locus_tag="KPB88_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054579.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7506..8057
                     /locus_tag="KPB88_002308"
     CDS             7506..8057
                     /locus_tag="KPB88_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054580.1"
                     /translation="MQSLNEIIAENLKKIRKEKRLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQENDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8143..9075
                     /locus_tag="KPB88_002309"
     CDS             8143..9075
                     /locus_tag="KPB88_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG0054581.1"
                     /translation="MNNKKAVFFFCSNLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGKSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYSEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKIGEKLNVPVFKHKILKNPKDLPIW"
     gene            9130..9993
                     /locus_tag="KPB88_002310"
     CDS             9130..9993
                     /locus_tag="KPB88_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0054582.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10240..12105
                     /locus_tag="KPB88_002311"
     CDS             10240..12105
                     /locus_tag="KPB88_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0054583.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDINDSTIKTEEKINLGARFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLNCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLAIMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAVVLPFMVATGMHKALIPYAINTIGKLGYEALYMTASLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGTFLG
                     LFTVKGFVLVGPGLASMSMFIDPNNGNNLIFAIIGFVVAILGSFIITLIIWKEDANTA
                     ESSTEDIVEEELIEEEKEVNILVSPVEGKVIDISKVNDELFASKTLGDGVAIIPANGN
                     LYAPCDSEVVMLFETKHAIGLKTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEG
                     DLLIKFDLDKISHANLDSTVMIVNNNGTDYAYKVLNKSYGNVKKGSILFDVKGGI"
     gene            12108..>13410
                     /locus_tag="KPB88_002312"
     CDS             12108..>13410
                     /locus_tag="KPB88_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0054584.1"
                     /translation="MKKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVIDMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWG"
BASE COUNT         5306 a         1323 c         2349 g         4432 t
ORIGIN      
        1 tagaaaaaaa gttatcttaa agtctaaaaa agcaataaaa gataactttt aactataatt
       61 atatagaatt ataactgtac atttattaaa ttttaatttt agaattattt ttgataacta
      121 tcttttttta tttatgaatt ttgttcctaa atattttttg atatcttctt caggagaaaa
      181 gtcatcaata agtatttctt tccctgtgtc ttcaaatact acaagaactt cacagtcacc
      241 ttgtaagaaa aattttctaa ctgtatcttc tttaacttct ctaactttta ttacattctt
      301 tttttctttt tcaaagtctt ttgtgtaata acttcccatc atattaatta tttttgtata
      361 tctattcata aaatacctcc atatgcgatt aaatttaaaa aatatgttat aataattgtt
      421 tgtattaact taataattat aacaaggagt aataataatg actagtataa aaaataaaga
      481 tattttaaat tgtatagatt attccattaa aaataagctc tttaacaaac taaatgatgt
      541 atataagtcc ttgccagcag gaaattgttc tggatgtgga aattgttgta tggagtcagt
      601 aggtataaat ttaattgagt ttttgaatat attttgctat cttgaagata gagttgattt
      661 aaaaaaaagc tgtataagta aagtattaga ttattatttt gaggagtata caagaaagaa
      721 cccatgtcca tttaaagata ctaataatag atgtttaata tatgaagtta gaccattaaa
      781 ttgtaggatg tttggacatt ggaaaaaaga ggattataat aaaaatttag ataatgttat
      841 tgagaaaaat aataactata aagagttaat gaaaaagcaa tatggatttg aaataaatga
      901 tgaagtagta aattacagga tagattattg tgatagattt attccaaata aagattattt
      961 aagcaaatct gagagattaa gcttttttga cgaacttatg acattagact caaatattta
     1021 ttcaagtgga aatatagata tagactttag ggatagaggt atagttgaat attttataga
     1081 gtcattactt tatagaaatt tagcatataa tgtgaaaata aggatatcta aagaatacaa
     1141 aataagaaaa agaactatag atagaataaa aaggattgtt cttatatagt ttcatattag
     1201 taagtaataa attgtatcat aaatattgtc aaaatgaggt gaagaaggtg aatcttacaa
     1261 aagtaaaagg aaataccttt tttataaaag gtggcacgaa tacaggaata tatgtatttg
     1321 aagataacac agcacttatg atagacccag ggttatgtgg ttcaagacct aggagagtaa
     1381 ataaagtatt agatgaaaat aatatacagg ttagatatat tataaatact catggtcatg
     1441 atgaccacta tggagcttgt aatcaattta aagagtatta taaagatgtg tgtataatgt
     1501 catctgaata cgataaatta tatatagaaa atccagaatt gttttcaaag tatattgttg
     1561 ggggaaagag caatgtattt atggataata tcttaaaaaa taaaatgttg gatgatataa
     1621 aaatagataa atctatagaa gctggaaaaa tagaattaaa taatgaaatg tttgatataa
     1681 ttgagttcca gggtcataca cctggtagta tagctgtttt aacgaaggat aatgtgattt
     1741 ttgttggtga tttattgata ggagatgaaa ttctatcaaa atacgatttc ttgtttctat
     1801 atgatataca agaacaaata aattcattag aaaaattaaa aaatatagat tttgagtact
     1861 tagttttagg gcatggcaag caagttatat caaaagaaga ttcttataaa ttaatcgaaa
     1921 aacatttgaa tgctttaaaa aaatatttat atcaaataag agagttgctt gtagaaccga
     1981 aatctttaga aattttatta aaaaacatta taaataacaa taatttaagc aataattata
     2041 aagagtatca tttttttaaa tcttcgttag tatctgtcat aagttactta gttgatttag
     2101 aagaaatagg gtatatattg gaaaatggag aattgcttta ctatacaaaa acaaaataaa
     2161 ttatgataaa ataaagaagg tcataaaaaa caaataaagg gtgagcaata tggaatacga
     2221 aaaatggaat gagatactag caccttatga acatgcagta gaggaattga aaataaaatt
     2281 taaaaatata agaaaagaat atttagcaaa aggtgaatat tctcccatag agtttgttac
     2341 aggcagaaca aaaaaaatat cttccataat atctaagtta aaaaggataa atgcaaaaga
     2401 tatagaaact gaaattgatg atattgctgg tataagaata atgtgccagt ttgttgagga
     2461 tatatatgct atagttgatt tgataaaagt aagaaatgat atgactataa taggagaaaa
     2521 agattatatt acaaattaca aagatagtgg ttatagaagc tatcatgtta taataaaata
     2581 ccctataaac tccattgcgg gttcaaagga aattatatgt gaaatacaaa taagaactct
     2641 tgcaatgaat ttctgggcta caatagaaca ttcattaaaa tataaatatg accattacat
     2701 acctgagact cttgctgaaa gacttagaag agcatctgat gctgcctttc ttttagacca
     2761 agaaatgagt gaaataagag aggacataat gaaagctcag gctatgtacc aaatgaagtc
     2821 tatagctatt agagatactc taaatagaat acaagagtta tatgacttgg gtgatacaca
     2881 taaagctatg cagtatcaaa gaaaacttga tagaactgaa acagataaaa atataactga
     2941 aattttggaa ttaaaacaag aaatagatat attactagag caatataagg atactaaggt
     3001 agatgataaa gtagttcagt aaaaaactac ttgtagaaaa tttgctaaat attaatgggg
     3061 ctgcctctta atggaaaata atttctaaaa agagacagcc tatttagtgt aatcaagaaa
     3121 ggaatgatat tatgagttta tatgctatag gtgatttaca tttttcaaca tcagtcaata
     3181 agccaatgaa tatctttgga agtaattggg atggtcatga aaagaaaatt atagataatt
     3241 ggaaagaagt tgttaaggaa gaagatatgg tcttagtact aggagataca tcttggggaa
     3301 ttaatttatc agaagctaaa aaagatttag atataataag caaacttcca ggtcagaaaa
     3361 tacttataaa aggaaatcat gattactggt ggacaaccgt tacgagttta aataagctat
     3421 atgaagacat gcgttttatt cagacaaatt tttatgaata taaagattat gcaatttgcg
     3481 gaggtagggg ctggatatgt cctaatgatg ttaaatttga tgagactgat gaaaaagttt
     3541 ataaaagaga agaacataga cttagattat cattagaatc tgctcgtaaa agtggtcact
     3601 caaaaataat tgtgattact cattatcctc ctacgaatga taaattagaa gaatctttat
     3661 ttacaaagtt atttgaagaa tataatgttg aaaaggtgat ttatggtcac ttgcatggta
     3721 aagaatcttt taaaatggga ttgaagggga ttcgaaatgg agtagagtat acattagcat
     3781 cttgtgacta tacagaattt aatttaataa aagttcacga ttaaaaaaca taaattggta
     3841 aatactataa gatacatgat taaaaataaa tatttgaggg atttcggttt tgagaaagga
     3901 gacacgactt atgaggtgga tattagtttt tatattttta tatcttattc ctttagtagt
     3961 gatattcaga aattacaaga attttaagag gtcttgtatc tacagtagta tttatgtggt
     4021 attagctaca acaataatga taagtaatat ttatatgagt ggacttaata aaataaaaga
     4081 atctatgtat tatcacaatt atatttctga tgagacatat gatgaaaagt atgtatcaaa
     4141 ttttaaggaa aaagaaagta ctgatttaaa acaggataag aaaaagaatg aagagatatc
     4201 aaataataaa aatttaaaca acaaacaaga tagaaatata caaaataaag aaagtaacga
     4261 agaaggaagt aataacataa aagaaaagaa taattctgat agtaatgttt cagtgaataa
     4321 taaatcagaa aaacaagaag atactacaac agaaaataat aaatatacaa gtaaagaaaa
     4381 aaatagtgaa gatataaaaa aaatagatat ggaaagtata ggaattttta agaaagaaat
     4441 ttataatata gaaagagcag ctttagttcc aatgagagaa tgtatacctt acactaagga
     4501 tatcgcaaaa aatataacta aattatctag tataaaaaaa gatgttacaa atgccagaaa
     4561 caagtgtaaa gaagttgtaa aagaatatga aaatatgcag attccagaac tttctaagga
     4621 gaagtataca gaaacattga gtaatgctag agatgacgta aagaaagcat atgagcttag
     4681 agaaaaatct atggaaagtg ctttgaaatt aataaatact aaaagcccta aatatatagg
     4741 gaagataaca gaatatcttg atttatcaga taagcagata gaaagtttta aaaatagatt
     4801 gaacgattta aaagaagaaa taaataatac agaaacaaat aatatcaatt aataagattt
     4861 aaggatataa gttaaaaggg agattatgat gaataaacag agtataggaa aactaaatat
     4921 gttttttatg gggattactc aaagaaacct tgataatagg gattttttta ttgagttaga
     4981 ggtaattttt aaatctggaa gaaaagagtt taaaggaatt gcaatagagg aagataataa
     5041 atataaattt aattttaaag gaaaatcaga tttatatacg tttaatgaat tgcttaagaa
     5101 tatttctaaa gaggcagaaa attatgatgg attagtattt aaatatgttg agcgaggaac
     5161 tagtgttgta attgaaggag acaataagaa ggtaaatgta aaatatttag ataataagga
     5221 agaagtacct aaaatagatg aatttacggc atctcaaata aaaaacagag attattatgt
     5281 aaaagtagga caagcaaatg cacttctaaa agaaattgga gtacttacaa aagatggtaa
     5341 gataaaaaat gataaaataa gaaaatacaa tcagatagac cattttgttg aattgataga
     5401 taatatttta aaagaaataa aggataaaga ctgtataact atcttggatt gtgcttgtgg
     5461 aaaatcttac ttaagctttg tcttaaattt ttatataaaa gaagtattaa agaaaaactg
     5521 ttattttata ggcatagatt attcagatgt agttatagaa gcatcaaaaa atatggctaa
     5581 aaacttgggt tataaaaata tgtcatttat aaaagaggat ttaactaatt atacacctaa
     5641 tagggatgtt gaccttgtaa taagtctaca tgcttgtgat acagctacag atatggcaat
     5701 aggtctagga attagagcaa aatctgaagc tatagtagta gtaccatgtt gtcataagga
     5761 gttattaggg caatatagat atgaagctat ggaaccaata ttaaaacatg gtgtatttaa
     5821 agctagattt gcagatttaa taactgatgg tttaagaaca ttgttattgg agggcaatgg
     5881 atatgatact tctgtagtag aatacatatc tccacttgat acaccaaaaa atttaatgat
     5941 aagagcgata aaaactaaaa cgaataatga caaagcatta aaagaatata aagaactgaa
     6001 atcacaattt ggagtggaac caaccttaga aaaacttata tattagttaa aaatgtaaaa
     6061 tttacgaaaa gcttgcttaa atatgcaagc tttttggtat tatataggta tagtcatatc
     6121 tagagttttg atttataatt aaagctgtgc tataatattt gattaatcaa atcaataaat
     6181 gttacatgga ggtaaaaatg aacaaattga atatatgtat tgatatagat ggaacaataa
     6241 caagccctta tcattttctt ccatacctaa atgatatctt taataaaaat ataactgaag
     6301 cagagtgtat aacacataat tgggaagaat tgtacggttc gggaataaga gatgtgtatg
     6361 cggagtttaa taataagtat atacactcat atgatgaagc aaagattgta gagggtgcta
     6421 cagaggtaat tagcacatta tctaaaggac acaatttatc ttttgtaacg gctagacatg
     6481 agtgtttaac tgatgtaact aaaaattggc tatcaagaca aggattttca gatatagatg
     6541 tttacttatt aggaagtgat tataaagttg aaaaggcaag agaactgagt tgtgacatat
     6601 ttatagaaga taatccttta aactcagtgc aactagctga tgatggtgtt aaagtaatac
     6661 ttctggatac taactataat aaagatgtta aacatcaaaa tatagttaga gttagtaatt
     6721 ggatagatat tgaaaagatt ataaaacaag gtatttaaag atttgtagat tgaaaggggg
     6781 agcatatgat agatttaaaa attgaaaata gaaaaggaaa aatccatgta aagagcagtg
     6841 aaataaaaga cattttagaa ttacgtccag acttcgaata tgtacaagat atatcaaata
     6901 ctataaatca agaaaatata ttggtatttg attgccagtt gacaggaaac tcttttgata
     6961 tggatgattt agatatagaa gaaatattgg aagagttagg tgaagaaata gatgagtctt
     7021 atttcagtgt tttatttgaa gatgtaagag cgtatttaaa agatgctact gatgagatag
     7081 aagcagactt acaagataat tacttatttg ataatttgag atgttatttt gatatataca
     7141 atataaatca agaatttaca gattttaagt ttgtattttt agtttctttt aaagatataa
     7201 aaatttcttc attagcaaac ctagctaaga ttgtttctaa gagacagctt ataggtgcat
     7261 ctaagtttta tagttaaaat gaaatattat tataataaaa gctagtagcc ttgtaaaaat
     7321 ataggttact agttttttat ttgttcattt tcacaaaatt tcagaaaact atttacattc
     7381 taaaaaaagt aatgtatatt aaacataatg tacattataa tggataaaaa gtttctatta
     7441 aattactaag tttatttaat aatcaaaaga atttatctta tattagtatg tattaggggg
     7501 tatttatgca aagtttaaat gaaataattg ctgaaaattt aaaaaagata aggaaagaaa
     7561 aacgtcttag tttagacaag attgcacaac ttagtggtgt aagtaagagt atgctatctc
     7621 aaattgaaaa gtgtgaggta aatcctacta tatctacttt aaagaaaata actaatggac
     7681 taaaaatctc atttacatca ttaatggaga ggcaggaaaa tgacatagaa ttgattcaaa
     7741 aaagtgatat agaccatttt attgaggata atggaaagta tataagttat ccaatatttc
     7801 catttgactc caaaagaaga tttgagatat ttatgattga aatagaagaa ggtggaaatt
     7861 tagattcaaa tgctgaactt ccaggaacac aagagtttat aactgttttt agtggcgaag
     7921 taaccattaa aataaatgga gaagattaca ttgtatctag tggaaattct ataagattta
     7981 aagctgatgt atcacatata tatagaaact caggcaaagg tatagctaaa atgagtatgg
     8041 tagtatatta tatataaata atttaatgga actagagtga tatttagatg attttaggta
     8101 tgaagatata ttatatttaa gttataggag tgtgatttat ggatgaataa taaaaaagca
     8161 gtttttttct tttgtagtaa tttaaagaaa gacccagtag ccagtaatgt gataaagtgt
     8221 tgtgaagaaa taatggattt aaatcaaact gatattgtta ttgatgataa ttttgtatta
     8281 gagtttaagg ataaaaataa taacttattt cattttgtaa aaacaaaaga tgtaataagt
     8341 cataattata aatattatct tccaatttta aatagacatt ttagagatta tgattttgca
     8401 ggagttgtaa attggcatga gggagaaaat gccccagaac atatcctaac agctcataca
     8461 attggagatg ttccaacagg agaatttgga aagtcaaatc caagatactt taaaaatcta
     8521 atcaatgcta ttgaggatat aaaaaatgaa aatctacttg atgaatttac tacattaaca
     8581 gaagcaactc actggtctgg aacaacatat agtgaagaat ctaaattaat tacagaatat
     8641 tctgtaccta tgttggatat tgagatagga agtagtagcg atagttttaa taatagtata
     8701 gcaatacagg tattggcaaa atcattaatt agagtatttg attgtgatga acctttgaag
     8761 actttattat gtgttggagg ggtacatttt gagaaatctt tctcagatat tataaaaaat
     8821 aaagaatata acatatcaat tggacatgta ttgccaaatc aatggattgt aagtggaatg
     8881 tatgatgatg aatcgggatt tgagaaacta gaaaagtgta taaatagtat tgaaggtgga
     8941 attgattgta tagtttttca tgataaatta aaaggtacct ataaagaaca atgtcgtaaa
     9001 ataggtgaaa aacttaatgt acctgtattt aaacataaaa tattaaaaaa tccaaaagac
     9061 ttacctatat ggtagtctta tacagttaat ataacaataa gtcggattaa tttttaagcg
     9121 aggtcaaaaa tgatattatc ttattttgca ttattaatta gagtagtgct attagctttt
     9181 gaaagagtag ttgtaagatt attaggtgat gaagaaggag atatttataa aaatatagct
     9241 tcatcatttc tatttttctt tataggtgga gtatgtttac ttccatttag cataatggat
     9301 agagttagtg attggtcgtt tttaatacca tgttatataa gtagtttagt ctattcaatt
     9361 ggttcagtag cgtatgtaac gtctttagca actggggaag tttcacttgt tactccaata
     9421 aatagtttaa attcattgtt tttactgctt ttatcagtta tatttttagg tgaaggttta
     9481 agtttagcaa aagttgcagg aatcataatt atgattggtg gagtgtttat attaaaaaaa
     9541 gtaagctctt ttttaaagaa tgtctctgta atttttaata atttaccatg tagattaatg
     9601 tttttataca tatttttgca gtcagtagga agagtattgg acaaggcttt ttttgtacaa
     9661 gtatctccta tattatattc aactatactg tattttttcg taggtttaaa tttatttatc
     9721 tttttagcat ttaaaaagaa acaaaaaata atttgtgaga tttttagtaa taaaaagggg
     9781 ctttcaattt taagtggagc tattaatgga tattcttatt tagcattatt aattgcttta
     9841 aataatctag aacttagtat agcagagcca ttttctcaag tatctatgat aataacttta
     9901 atacttgctc attttatatt caaagaaaat ataaaagaaa aaataccagg ttcaattttg
     9961 atactaattg gtgggtggtt actgttactt taattgatat aaaaaatgaa ttataaaggt
    10021 gctattaatt agtatcagaa aatttaaaaa agtattgaca attctcttag gcaaatgtta
    10081 atatgataat acaaaatagt ataaatagaa gacatgttac tggtaatgca ggcaggatcg
    10141 aatatattgc tttgattttt caaggtgttt atataggttc tgcctttttt gcatcttgaa
    10201 tggccattca taaaaaatta tggagaagga gattacaaga tgaattataa aaaaactgcc
    10261 attgacatta ttgaacaagt tggtggttca aaaaatattt tagctttaga acactgttca
    10321 actagactac gctttaagtt gattgataga agtaaagtca atcaggatgc tttaaaaaaa
    10381 gtacaaggtg ttatgagtat agttaatggg gcacagtttc aagttgtcat tggaaatagt
    10441 gttgttgatg tttatgatga aattttaaaa gtttgtcctt tagatataaa tgatagtact
    10501 ataaaaactg aagaaaaaat aaacttagga gcaagattta tagaatttat tattaatatt
    10561 tttcaaccat tgatttttgc tattgcagga gctggtattt taaaatcact gttgatgtta
    10621 ttagcaatgt ttaatatttt aaaatcagat tctactatat atacattatt agttgctatt
    10681 tcagatgcaa cattctattt cctacctctt atggtagctg taactactgc caatgtattg
    10741 aactgtaatc gtttagtagc tattgccgct gtaggatact tattgcttcc agcaacaaca
    10801 actgctttaa gtgagggtgt cgaactattt ggatttacaa ttccaaacat tgcctataat
    10861 gcacaggttt tcccagctat cttatgtgtg tctttcttgg caataatgga gaaaattttt
    10921 aacaagtatt caccaaaacc aattcgaact ttctttgttc ctatgatgtc tttggcaatt
    10981 actgttccaa ttactctaac tattttaggt cctttaggat ataacgttgg tacaattttt
    11041 acaaccatta ttttattctt atataacaaa atgggattct tagttgtagg attacttgct
    11101 gttgttcttc cgtttatggt tgcaacagga atgcacaaag cattaatacc ttatgcaatt
    11161 aatacaattg gaaagttggg ttatgaagca ttatatatga cagcttcatt agctcacaat
    11221 atttccgaaa gtggtgcttg ttttgcagta gccttacgta caaaagatga aacattaaaa
    11281 caaacagcct tatctgcagg aatttctgcc ttgatgggaa ttacagagcc agctttatat
    11341 ggtattactt tacaacataa aagagcaata ctaggtgttg tattgtcaag tgcgatttct
    11401 ggtacatttt taggattatt tacagtaaaa ggatttgtac ttgttggacc agggcttgcc
    11461 agtatgagca tgtttattga cccaaataat ggaaacaatt taatttttgc aattataggt
    11521 tttgttgttg caatattagg ctcatttatt attacactga ttatctggaa ggaagatgct
    11581 aatacagcag agtcatcaac tgaagatatt gttgaagaag aattaataga agaagaaaaa
    11641 gaagtaaata tcttagtatc tccagtagaa ggaaaagtaa tagatataag taaggttaat
    11701 gatgaattat ttgcaagtaa gactttggga gatggagttg caattatacc agccaatgga
    11761 aatttatatg caccttgtga ttcagaagtt gtgatgctat ttgaaacaaa acatgcaatt
    11821 ggtttaaaaa caaaaaatgg ggctgagatt ttgattcata ttggaataaa tactgtaagt
    11881 atgaatggag atggatttaa aacatttgtt aagacaggcg ataatgtaaa agaaggagat
    11941 ttgttaatta agtttgattt agacaaaatc tcacatgcta atttagattc aactgttatg
    12001 attgtaaata ataatggaac agattatgct tataaagtgt taaacaagtc ttatggcaat
    12061 gtaaaaaaag gaagtatctt gtttgatgta aagggaggaa tataaatatg aaaaagtttc
    12121 cagaaggatt tctttgggga ggagctacag ctgctaatca atttgaaggt ggttggaatt
    12181 taggtgggaa agggtggtct gtatcagatg tagcaaaggc acactttgat gtagatgtga
    12241 aagactataa atctaataat gaaatcacaa caaaagatat agaagaagga ttagcacatc
    12301 ctgaagatga agtaaattat ccaaaacgtc atggtagtga tttttatcat cactataaag
    12361 aagatattgc tctaatggca gaaatgggat ttaagacata tcgaatgagt attgcttggt
    12421 ctcgtatttt tccaaatgga gatgataaag aaccaaatga agaaggttta cagttttatg
    12481 atgatgtatt tgatgagtta ataagttatg gaattgaacc acttgttact atgtcacatt
    12541 atgaaccacc attaaatatt gttttaaatt atgatggatg gtattcacga caagttatag
    12601 atatgtttgt taggtatgta gaaactatct gtgaaagata taaaaacaaa gtgaaatatt
    12661 ggcttacatt taatgaagtt gattctatga ttcgtcatcc gtacactact ggtggtttgg
    12721 ttcgagaccg ttttaaggat aaaaactttg aggaagtcat ttttcaagct atgcatcatc
    12781 aatttgtagc tagtgccctt gcaacaaaaa tttgccatga gattattcca aattcaaaag
    12841 tagggtgtat gttaacaaaa ttaacatatt atccatatac atgtagacca gaagatgttt
    12901 tggcagctca acaaaaaatg agaagtattt atgcatatag tgatacacag gtttttggtg
    12961 agtatccagt atatttactg tcgtatttta aaaataataa cattcaaata gcaaaagaag
    13021 aacatgatga tgaaattatg aaaaagtatc cagtagattt tatttcattt tcttactata
    13081 tgtcttcttg tgaggcagct gatacaactg gattagatat tacacctggg aatacgttat
    13141 tagctgtaaa aaatccttat ttagaaatga gtgaatgggg ttggcaaatt gattcaatag
    13201 gtctacgtat atctttgatt gaattatatg atagatatag aaagccttta tttatagttg
    13261 aaaatggatt aggagctaaa gatattttaa ctgaagataa aaaagttcat gaccaatata
    13321 gaattaatta tttaaaagaa cactttaaat gtatgttaga tgcgattatc gaagatggcg
    13381 ttgaactttg gggatacact tcatggggtt
//
LOCUS       DAELJO010000096        13401 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELJO010000096 DAELJO010000000
VERSION     DAELJO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.096"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            211..1407
                     /locus_tag="KPB88_002313"
     CDS             211..1407
                     /locus_tag="KPB88_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG0054585.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1500..2024
                     /locus_tag="KPB88_002314"
     CDS             1500..2024
                     /locus_tag="KPB88_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG0054586.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2049..2225
                     /gene="rpmF"
                     /locus_tag="KPB88_002315"
     CDS             2049..2225
                     /gene="rpmF"
                     /locus_tag="KPB88_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG0054587.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2432..2989
                     /gene="fapR"
                     /locus_tag="KPB88_002316"
     CDS             2432..2989
                     /gene="fapR"
                     /locus_tag="KPB88_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG0054588.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            3000..4022
                     /gene="plsX"
                     /locus_tag="KPB88_002317"
     CDS             3000..4022
                     /gene="plsX"
                     /locus_tag="KPB88_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG0054589.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSNGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4048..5034
                     /locus_tag="KPB88_002318"
     CDS             4048..5034
                     /locus_tag="KPB88_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG0054590.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5143..6072
                     /gene="fabK"
                     /locus_tag="KPB88_002319"
     CDS             5143..6072
                     /gene="fabK"
                     /locus_tag="KPB88_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG0054591.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6088..7038
                     /gene="fabD"
                     /locus_tag="KPB88_002320"
     CDS             6088..7038
                     /gene="fabD"
                     /locus_tag="KPB88_002320"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG0054592.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7038..7787
                     /gene="fabG"
                     /locus_tag="KPB88_002321"
     CDS             7038..7787
                     /gene="fabG"
                     /locus_tag="KPB88_002321"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG0054593.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7841..8065
                     /gene="acpP"
                     /locus_tag="KPB88_002322"
     CDS             7841..8065
                     /gene="acpP"
                     /locus_tag="KPB88_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0054594.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8094..9332
                     /gene="fabF"
                     /locus_tag="KPB88_002323"
     CDS             8094..9332
                     /gene="fabF"
                     /locus_tag="KPB88_002323"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG0054595.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9558..11276
                     /locus_tag="KPB88_002324"
     CDS             9558..11276
                     /locus_tag="KPB88_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0054596.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11307..13307
                     /locus_tag="KPB88_002325"
     CDS             11307..13307
                     /locus_tag="KPB88_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054597.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
BASE COUNT         5433 a         1391 c         2625 g         3952 t
ORIGIN      
        1 acaatttacc atactagaaa tatgatataa tataaaaaaa aagtatatac ttttatatta
       61 aaaggtgata tattatttat gaaaatcgtt taatgtatac aataacatat ctataacaaa
      121 ataataaaag atagagtttt atttagacat ttattacaaa tcaaaattta ttacatatta
      181 tttataaaaa aatctaagga ggaaataata atgaaaatat tagtattaaa ctgtgggagt
      241 tcttcattaa aatatcaatt aatagatatg aataatgaag aagttttatg tataggattg
      301 gttgaaagaa taggaataga aggttctata ttaaaacatg aaaaagcagg tagagatgat
      361 aaatacgttg ttgaacaacc aatgaaggac cacaaagatg caatagcatt agttttagaa
      421 gctgttgctc atccagaatt tggtgcagtt aaagaaatga aagaaataga tgcagtagga
      481 catagagttg tacatgctgg agaaaaattt gcaacttcag tagtaataac tccagaagta
      541 gaagaagctt taaaagaatg tatagattta gctccacttc ataatccagc aaacataatg
      601 ggaatagatg cttgtaaagc tatacttcca gatgtaccaa tggtaggtgt atttgatact
      661 gctttccatc aaacaatgcc taaatcttca tacttatatg gtttacctca tgagctatat
      721 actaaatatg gggtaagaag atatggattc cacggaactt ctcataatta tgtatctcaa
      781 agagcagcag agatactagg aaaagatata aaagatttaa aaatagtaac ttgtcactta
      841 ggaaatggtg cttctatagc tgctgttgat ggtggaaaat gtgtagatac atctatggga
      901 ttcactccat tagaaggatt aataatggga actagatgtg gagatataga cccagctata
      961 ttaccattct taatgagaaa agaaggttta gatgctgatg gattggataa attaatgaat
     1021 aaagaatctg gagtatatgg aatgactgga atttctagtg acttcagaga tatagaagat
     1081 gcagctaaaa atggagacga aagagctcaa gctacattag aagcgtatgt taaaaaagta
     1141 caaaaatata taggcgctta tgctgctgaa atgaatggat tggatgttgt agtatttact
     1201 gcaggcgttg gtgaaaatgg aaaggctata agagcagata ttgcttctaa catggagttt
     1261 ttaggaatga aattagataa agaagctaat gatgttagag gaaaagaaac agtaatatct
     1321 actgctgatt ctaaagtaaa aatgctttta ataccaacta atgaagaatt aatgatagct
     1381 agagatacat taagattggt aaaataatta aattaaacct atgcctatca agtaaaaata
     1441 cttgacaaac aaatgctaga tttgtataat aaatttggtg atatgttgag gtgaacttaa
     1501 tgaaagttag catagaaaaa ataaatagaa aagaaactga caaaatagac ttgaattttt
     1561 gtgaaaaaat tgatactatt agttattgtg atgagattta caaattagta tcacctgtaa
     1621 atttaaaagg gaaagtgtca aagactaata aaggtttgta tctagatata gatgtcaatt
     1681 ttacaattgt tgacaattgt tcaagatgtc ttaaagaagt agaaatacca ctagagtatt
     1741 ctatacaagg ttttttagta aaagaagaag attatgatga agatgagttt gaggaatttg
     1801 atccttttat attcgatggt gaagaaatcg accttattga tataatagaa cagacattag
     1861 attttaatgt acctcataaa gttctttgta gtgaaaactg taaaggtctt tgtcaagtat
     1921 gcggggcaaa cttaaatgaa gaagagtgtt cttgcagtga aattacaaac gatgaggaat
     1981 acatagatcc tcgttttgct aaattaaaag atttatttaa ttaaattttg actaaggagg
     2041 tggcgtaaat ggcagtacca aagcgtaaaa cgtctaaatc aaacacaaaa atgagaagag
     2101 cagctaactc aaagatggaa gcaacaggat ttgtaagttg cccacaatgt catgagccaa
     2161 aattaccaca tagagtgtgt ccagactgtg gatattataa aggtaaagaa gttgtgtcta
     2221 agtaatagct tctaaaaagc atgtagagta tactctacat gttttttttg tacatataaa
     2281 aaaataagat gatttactag agaaaataag tggattttat gcatattaaa aaatagaaaa
     2341 ataataatat aagtattgaa aatttttgtg tgttactata tactactatt agtagctggt
     2401 actaattaca gctataaaat ttggagggaa catgaaaaag aaaagtaaag cccaaagaca
     2461 aaaagagctt atagatatgc taaagacaga tcctttttat actgatgaag aattatcaag
     2521 tctgtttgat gtaagtatac agaccataag attagataga atgagtctaa acataccaga
     2581 gctaagagaa agagtcaaat caatagcaga aactcagagt tcaaaagtaa agactttagg
     2641 agtaaaggaa attacaggag aaatcattga tttatctgta gggcgattag gtatatctat
     2701 gttagaagtt acacaggaca tgatttactc taagactaat actttaaaag atacgtatat
     2761 tttttctcta gcagattcat tggcaatggc tataatagat gcacctaagg ttattatgag
     2821 agttgcaaat gtaaaaagtt ttaagttgat agaacaacaa gataggctta tagcaaaagc
     2881 agaagtgtat agaaatatag ataaaaagca ttatgtaaaa gttgttatca acaataaagc
     2941 ccaagagcaa atatttagag gaaagtttat ctttgaagaa ttagattagg agggaaatta
     3001 tgaaaatagt aattgatgga atgggtggag ataatgcacc taaatctaat gtagaaggcg
     3061 cagtaaatgc tataaaagaa tatcaggttg atttaataat cacaggagat aaagatttac
     3121 ttgaaaagga attttcaaat tatgaatttg atagaaataa attagagata gttcatacta
     3181 ctgagattat agaaaacgaa gataaaccag taaaggctat aagaagtaaa aaagattcat
     3241 ctatggtagt agcattaaat cttgtaaaag aaggaaaggc agatgctata atatcagctg
     3301 gaaatacagg agccttactt gctggtggat tatttgttgt tggtagaata aaaggaatcg
     3361 atagaccttg cttatgttca gcaataccaa atgtaaagcg aggaatgaca cttatagcag
     3421 actgtggcgc aaatgcagat tgtaaaccta agaatctagt tgaatttgca gctatgagta
     3481 atatttattc aagaaaagtt ttaggtcttg aaaatcctaa ggtagcatta gcaaatgttg
     3541 gactagaaga aggcaaaggc aatgatttag tcaaaaggtc atatgaagaa attaaaaaat
     3601 tagatttaaa ttttataggg aatgtagaag ctagagaagt tataaatgca tacacagata
     3661 taatcatatg tgatggattt acaggtaata tattacttaa atctgctgaa ggtgtagcac
     3721 tttcagttat gagtcttata aaggaaactt ttatggccag tacaaaaagt aaaataggag
     3781 cattacttat aaaggatgat ttaagaaaat taaaaagttt tatagattat tctgagtatg
     3841 ggggagctcc acttttaggt ctaaatggag gagttataaa agctcatggt agttctgatg
     3901 ctaaagctat aaaaaatgct ataaatcaag gaataaaatt ttcaaatgga aaagtcgttg
     3961 aagatattaa ccaatttata agcaaataca atgaagaaaa taaaaataac gaagatgaat
     4021 aaactcgttt tggaggttta atatataatg aatacaaagg ctggaatatt aggagtagga
     4081 agttatctac ctgagcaatc atatgataat tttcacttcg aaaaaataat ggatacatca
     4141 gatgagtgga taagtactag aactggtatt aaggaaagaa gatttgccaa agagtctgaa
     4201 gcaacttctg acttagcatc taaagctgcc ttaaaagcta tagaatgtgc aaagttaaac
     4261 gtagaggata tagagctgat tatattagct acaattactc ctgatatgtc tttgccttca
     4321 actgcatgta tagtacaaga tgcaatagga gcagtgaatg ctactgcatt tgatatatca
     4381 gcagcttgtt ctggatttgt atatggagta actattgcaa agcaatttgt ggaaacaggt
     4441 tgctataaaa atgtgcttgt aataggagca gaaacttgtt ctaagttttt aaattatgat
     4501 gatagaacaa cagcagtatt gtttggagat ggagctggag cagctgttat tggccctgta
     4561 aatgagggtg gaatactgtc tacacatatg ggctctgatg gtaaaggaaa agattgttta
     4621 aaagtacctg ctggaggttc aagacttaag gcaagtaaag agactgtaga agctaatcta
     4681 catacaatag aaatggcagg tagtgatgtg tttaaatttg cagttagaaa gatggcagaa
     4741 acttctttaa gagctcttga aaaagcaaat ctaaatacaa ctgatataga ctacttagtg
     4801 ccacatcaag ctaatataag aatcatacaa gcttcttcta agagattaga gcttgatatg
     4861 aaaaaagttt atgtaaatat agacaaatat ggaaatatgt ctgctgcatc tataccagta
     4921 gcattagatg aagcttatag agaaggtaaa attaaaaagg gtgataatgt agttttagtt
     4981 ggctttggtg gaggtctaac ttggggggct tcagttgtca agtggacttt atagtataaa
     5041 atatctaaaa tgatttaatt aacaaaatta aacttataca caaaataaag ttgattaata
     5101 ataaaaagtt tacttttatg atttcaagtg aaggagaatt gtatgaataa aatttgcaaa
     5161 atattaaata taaaataccc agttatccaa gggggaatgg catgggtagc tactgcatca
     5221 ttagcaagtg ctgtatctaa tgcaggagga cttggcataa tagcagcagg aaacgcacca
     5281 aaagaagcta taaagaaaga aattgttgag tgtaaaaaat taacagataa accttttgga
     5341 gtaaatgtaa tgcttatgtc gccatttgtt gatgatataa ttgatttgat tatagaagaa
     5401 aaagttcaag ttattactac tggtgctgga aatcctgcaa agtatatgga tagattaaag
     5461 gaagctggaa caaaggttat tcctgtagta cctacaatag ctttggcaca aagaatggaa
     5521 aagctaggag ctacagcagt aatagcagaa ggtactgaag gtggaggaca tataggagaa
     5581 cttactacta tggtcttagt tccacaagtt gctgatgctg taaacatacc tgtaatagct
     5641 gctggaggaa ttgtagatgg tagaggaatt gcagcatcat ttgcattagg tgccagtgca
     5701 gttcaagtag gaactagatt tatttgcagt gaagagtgtt ctgtccattc aaactataaa
     5761 aacttagtac taaaagcaaa agatagagat gcaattgtaa caggaagaag tactggtcat
     5821 ccagtaagaa cattaaaaaa taaactatca aaagaatttt taaagatgga acaaaatgga
     5881 gctactcctg aagaactgga taaaaaaggt acaggagctt taagatttgc aacagtagat
     5941 ggagacatag aaaaaggttc atttatggca ggtcaaagtg ctgctatggt aaaagaaata
     6001 acaccttgta aggaaattat agaggctatg gtaaatcaag caagagagat tatgccagca
     6061 atagaactgt aaatcaggag gagcaagatg ggaaaagttg cactagtatt ccctggtcaa
     6121 ggagctcaat atgtaggtat ggccaaggat ttatatgaaa ataatgctgt tgctaaaagt
     6181 gtaatagatg aggctagtga tgccttaaat atggatttaa aaaaccttat gtttaatgga
     6241 aatgaagaag aactttctaa aactgaaaac actcaacctg ccatggttac acatagtgta
     6301 gcagtattaa aagcggtaca agctcaaata gatttaaaat ataatgcatg cttaggatta
     6361 tccttaggag agtatagtgc gttggttgca gcagatgcaa ttgactttaa agatgctgta
     6421 tgtttagtta aaaaaagagg taagtttatg caggaaacag ttccagcagg tgttggagct
     6481 atggcagcag ttttaggttt agatagaaat gtacttgaaa atgtggttaa agatatacaa
     6541 ggaggtattg tagaagtagc aaattacaat tctcctggac aaatagttat atctggtgaa
     6601 aatgaaaaga tagaagaagc aatggtaaaa tgcaaagaag caggagcaaa aagggcagtt
     6661 aaattaaatg taagtggacc ttttcactca tcaatgttaa aagaagctgg tgtaaaattg
     6721 gccagtgaat tagaaaaagt taacataact aaacctaaag ttgatgtggt ggcaaatgta
     6781 aatgcagatt actataaaaa cgaaggtaaa gacctgctta taaaacaagt aagttcatct
     6841 gttctttggg aagattctat agaaagactt ttaaatgatg gatatgatac ttttatagaa
     6901 atgggacctg gaaaaacttt aaaagcattt attaagaaga tagcttccaa taaaaaagca
     6961 aatgtcaata tttataatat agatggtata gattctctta atgaatttgt acaaaattat
     7021 agaaatggag agatataatg ataaatctta caggacaagt agcagttgtt actggtggct
     7081 ctaggggtat aggaaaagaa atagcaaaaa aactagcatc ttttggagct gatgtagtaa
     7141 tcaattatac ttctaaagaa gatgaagcac taaaaactaa aaatgaaata gaaagtatgg
     7201 gggtaaagtg tacctctata aaatgtgatg tatctaaatt tgatgaagtg aatcaaatga
     7261 tagattctgt tgtaagcgaa tttggaaaaa ttgatatatt ggttaataat gcaggcataa
     7321 ctaaagatgg tctgcttatg agaatgaaag aagaagattt tgatagagtt atagatataa
     7381 acttaaaagg tgtctttaat tgtacaaaag cagttactaa acctatgatg aaaaagaagt
     7441 atggaagaat aataaatatg acttcagtag ttggaattat gggtaatgca gggcaaacta
     7501 attattgtgc atcaaaagca ggtgtaattg gatttacaaa agcttctgca agagagttag
     7561 catcaagaaa cataaatata aatgcagtag cacctggatt tatagaaaca gatatgacta
     7621 aagtactaag tgatgatgta aaagaatcaa cactagcaaa cataccaaag aaatcttatg
     7681 gtaaaccaga agatgtagcc aatgccgtag catttttagt tagtgacatg tcaagttata
     7741 taacaggaca agtaataaat gtagatggtg gaatggtaat gcaataataa tttaaaaagt
     7801 agagaaatct attaaaataa aaaattttag gaggtacaca atgtttaata aagttgtaga
     7861 aataataata gagcaattag gagtagaaga taaagaaata actatggaaa catcattaat
     7921 gaaggattta gaagcagatt cattagatgc agtagaaata ataatggcac ttgaagatga
     7981 atttggtata gaaataccag atacagaagc agaaaacttc aaatctatag gagatatagt
     8041 taagtatata gaagctaata aatagtcgag ctttaaaaaa gtggaggtat ccgatgaata
     8101 aaagagtagt aataacagga cttggatgtg ttacgccgtt gggaacagga aaagaagagt
     8161 tctggagtaa tataaaatct ggagtatctg gtatagataa aataactaat tttgatgcta
     8221 gcacatatca aactcagata gcaggagaag ttaagaattt tcatccagaa gaatatataa
     8281 gtaaaaaaga attgaagaga ttagataaat ttgctcaatt tgcaatagta tctgctaaat
     8341 tagcagttga agatgcaaat cttgatttag ataaagttga tagagaacgt tttggtgtta
     8401 taataggttc tggaataggt ggagttgaag ccatagaaac tcaacataaa attcttttgg
     8461 aaaaaggaaa taaaagagta agttcacttt ttgtacctat gatgatagga aatatggcag
     8521 ctggtcaagt ttcaatattt ttaggagcaa aagggcctaa cactaatgta tgtacagctt
     8581 gtgcatcagg aactcattca ataggtgatg cttttaaggt aattcaaaga ggtgatgcag
     8641 acattatggt agcagggggt tctgaagctg ctgtaacagg tcttgctttt gctggattct
     8701 gtaacatgaa ggctatgtca actagaaatg atgaccctaa aactgcatca agaccatttg
     8761 ataaagacag agatggtttt gtaatgggag aaggagcagg tattgttatc ttagaagact
     8821 tagaacatgc tttagcaaga ggcgctaaaa tatatgcaga agtagttgga tatggtttaa
     8881 ctgctgatgc ataccatatg acaactccag ctgaaaatgg agagggagca gctaggtcta
     8941 tgaatatggc tttgaaagat ggaaatgttc cattagaaga agtagattat ataaatgctc
     9001 atggaacttc aacttattac aatgatttat atgaaactat ggctataaaa actgtatttg
     9061 gagaaaaggc atatgactta tgtgtgtcat caacaaaatc tatgactggt cacttattag
     9121 gagcatcagg tgctatagag gctgttgttt gtgctatgag tatagaagat agttttgtgc
     9181 ctccaactat aaacatacaa gaagttggtg aagatttgga tttagattat gttccaaatc
     9241 aaggtaaaga aaaaaatatt agatatgcac tatcaaattc tttaggattt ggtggtcata
     9301 atgctacaat agtactaaaa aaatatgtat aaataaatat taaatataaa actaaaaaac
     9361 aagcacttgt tttaactgta taaatttata ttagcttaaa tttaaagtct cctaaattat
     9421 tttaggagac ttttttatta agatttaagt ctaggatatg ttgtaaatca ttttgatata
     9481 ttgtattatt aattattaag aggttattta tatatgcaag ttttcattct acaatataca
     9541 aaaggaggac taaaattttg aattttagac taccaaagaa aaggttaccc aaaaagataa
     9601 tttttatatg tataatactt attagtatta ttttgtttat aaagtatgac caaaatggat
     9661 tgaaagaaag agaaattata aataagggaa aatatatgtc tgaaagcctt gtggcagata
     9721 aggagtatga tgaagcaaaa aaagtggtta agacttcttt tgaatctatt ccagaaaaaa
     9781 aatacaataa agtatataat gaagtttggg atatgttaaa gactatttca tatgttccag
     9841 atggaacgaa gatagtcatt gattctttag aaaagatgag agaatcagat gtgaaattat
     9901 ctgatgaatg tagatttaat atagaaaaaa gattagcatc agtgtatata atggataata
     9961 attattctaa ggcagtagat ttaactgtaa agtctataaa gttggctgag aaattgggga
    10021 ataattatga aaaagcaaga ctagatgttg atttggcgag catattttta aagataggtg
    10081 gctatgaaac aggagagaaa ttaataaagg attcttttaa aatagatatt gatgaaggtg
    10141 aaaaaaatgg aatggttaga ttatatgcta ttataaatct agcagaaata tatgtagaag
    10201 agggagaata tgataaagct attgaaataa gtagcaggat aaaagattat aaaaagtttg
    10261 taaatacgga taattacaat gattttgata taatggcctc tataatgcag tcaaatgcgt
    10321 atgtgggcaa aaataatata caaaaggcaa agactttttt agaaaaagct gattatctta
    10381 taaaatcaga tagaacagtt tatatattag ataaagatat gtactattat atggcaatgg
    10441 gaaacttaga atataaaagc gaaaattata attctgcaat agataattat aaaaaaagtt
    10501 tagagatatc taccaaaaga aagttaaccc aggagaaaat aaaaaattta aataagatat
    10561 taaatatata tgaaaaacaa ggaaatatag aatctttaaa tgagtatcaa aagatgttaa
    10621 taaatgaata taaagagaac aaaagtataa gagattcaga gactagtttc tatataattg
    10681 ataaagtatc aaacgaaagt aagctatttg aaaaaactaa aaaagaaata aaatattata
    10741 aaatattatt tacagtaatt ttaattgcta tattagggtc tactttttta tacaaccgat
    10801 tgaagtattt taaagcacaa aatttacatg atggtcttac aaatgtttat aatagaaaaa
    10861 gttttgatat tatgtatgaa aagtacagag agaaagatga taattttgca cttgtgatga
    10921 tagatattga taatttcaag ctcataaatg ataattatgg acataaattt ggtgacactg
    10981 ttataaaggg aataacaaat accatttgtg ttatgttaga acaagatgat aaagtcttta
    11041 ggtacggagg agaagaattt tctgtattaa taagaaataa atctggtgag gaagtagcag
    11101 atataattga cagtataagg gttgcaattg caaataaaaa gtggaatgat gatgtgactg
    11161 tgactataag tgcaggtgta gctcatgttt cagatagcaa agatgtacta gaagaagctg
    11221 ataaaaattt atataaagca aaacaacttg gtagaaataa agtagtatat aagtaattat
    11281 atactatata aataagaggt ataaatatga aacaatcaat agctatagta actgataaaa
    11341 taactaaact atcatctttc ttagaagaaa atatacgttt ggttttaggt aattatattg
    11401 atataaacca ctactatctg gaagttttaa aagagaatga taaaatacaa ggtgattttg
    11461 tactagtaat gaatgatgat agattaaaca gtatgagaaa gcatttagta caagatagta
    11521 agattatagt gataaggaga acacttaaag aaaatgaaat atatggatta ttttcaatac
    11581 caaatggaac tgaagtattg gttgtaaatg atacaaaaga aactacatta gaaacgataa
    11641 gcttatttta caaaatagga gtaaaaaatt taaagtttac tccatatatt gaaggaaaaa
    11701 tttataaaaa tatagacata gctataacgc ctggagtaaa gggaaaagta ccaagttata
    11761 taaataaagt aatcgacctt ggaaatagat atattgatat atctaccttt atagaaataa
    11821 taaatggtct tagaatagat gttaaagaaa tacgtaaaaa tttaatggca tattctgaga
    11881 gtttaataag tttagataat ggagttaaag ataattatag gcaactgttt ttaaaaatag
    11941 aagaacaaga tgttatattg aatctatcaa aagatggaat aatatttact tcagcagatg
    12001 gtataataaa cacatttaat aaagaagctt taaaaatttt aggtataaat gaaaatccac
    12061 aaggcaaaaa catagaagat atcatttctg aagatttaaa tattttactt gaagacaaag
    12121 aaataattga tgaagtggta tttacaaata aaaaatatat aaatgtaaat aaaaaaagta
    12181 ttttttctat ggggaataaa gctgggatat attataactt acaagaaata acgtatataa
    12241 aaaaattaga acaaaatctt actaataaat taagagaaca aggtcagata gcaagatata
    12301 catttaaaga tataaaaact aaaaataaaa atatggtaaa gtgtgtagaa ttggctaaaa
    12361 aaatatctaa atcagacctt tctgtattaa taataggaga aagtggtact ggtaaagaat
    12421 tattatcaca atcaattcat aatgcttcca atagaagtaa tcaaccgttt atagcagtta
    12481 attgtgcagc agttccagat agtctattag aaagtcaact gtttggatat gaaaaaggct
    12541 cttttacagg agctttaaga gaaggaaaaa agggtctatt tgaacttgct aataatggaa
    12601 ctatatttct ggatgaaatt ggagatatgc ctccattgct acagacaaag ttactaaggg
    12661 tattacaaga aaagcaggtt atgcctatag gttcccataa tgttataaac atagatgtta
    12721 gggtcatagc agcaacaaac aaaaacttac taaagatgat tagagaagga aaatttagag
    12781 aggacctgta ttatagacta aatgtattgc caatagatgt accatcattg aggaatagaa
    12841 aagaagatat aataacactt atgaatttct ttttgaaaaa caatataaga ctttcgccag
    12901 aagttataac tattttagaa aaatataatt ggccaggaaa tataagagag ttacaaaatg
    12961 ttgcttctta tgtaagtcta atgtgtgata gtatggtttt tagagatgac ttaccaccat
    13021 atataagggt agaatgtgct aacaattatg aagatggatt tggttataca agaaaagaat
    13081 ttgaggaaat actgaggatt ctatatgaaa gaagagagtc atcctctgga attggaagag
    13141 ggtttataat cgaaaagctt atacaggaag gtattgagat ttctgaaagc aaagttagaa
    13201 aggtgctatc ttatttaagt gaatgtggat acatatcatc taaatctggt agaggaggaa
    13261 gtcaaataac ctcagagggg ataaaacttt ataataatat aaattaagct aagtatttga
    13321 tattgaaatc acatattttg aatttagata tttaaataat aataaacttt tatatttata
    13381 gagattaatt tatattatgt c
//
LOCUS       DAELJO010000097        13371 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELJO010000097 DAELJO010000000
VERSION     DAELJO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.097"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            61..474
                     /locus_tag="KPB88_002326"
     CDS             61..474
                     /locus_tag="KPB88_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054598.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            544..960
                     /locus_tag="KPB88_002327"
     CDS             544..960
                     /locus_tag="KPB88_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054599.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            961..1173
                     /locus_tag="KPB88_002328"
     CDS             961..1173
                     /locus_tag="KPB88_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054600.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            2217..3050
                     /locus_tag="KPB88_002329"
     CDS             2217..3050
                     /locus_tag="KPB88_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0054601.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            3176..5017
                     /locus_tag="KPB88_002330"
     CDS             3176..5017
                     /locus_tag="KPB88_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0054602.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            5040..6473
                     /locus_tag="KPB88_002331"
     CDS             5040..6473
                     /locus_tag="KPB88_002331"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0054603.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            7178..8056
                     /locus_tag="KPB88_002332"
     CDS             7178..8056
                     /locus_tag="KPB88_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG0054604.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            8092..9987
                     /locus_tag="KPB88_002333"
     CDS             8092..9987
                     /locus_tag="KPB88_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054605.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            10255..12162
                     /locus_tag="KPB88_002334"
     CDS             10255..12162
                     /locus_tag="KPB88_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0054606.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSESNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            12152..12601
                     /locus_tag="KPB88_002335"
     CDS             12152..12601
                     /locus_tag="KPB88_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054607.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            12701..>13371
                     /locus_tag="KPB88_002336"
     CDS             12701..>13371
                     /locus_tag="KPB88_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054608.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTG"
BASE COUNT         5335 a         1334 c         2378 g         4324 t
ORIGIN      
        1 aagttatata tttataacat aaggtgttat aaatatataa cttttaaggg gtgattttat
       61 atgtctaaat cagagcttgg tgttaaggca aatcttgttt cagagttaat gatgctatcc
      121 tattttttta tagacttgtt attacaaaag tttattttta aaggagaagg tatttggttt
      181 acttttataa ttttattagc tttttgggtg tgcaatctca tgtataataa aaaatataat
      241 gatttagttg atgaggcatc aaaacttatg ttgttaaagg ttgatcagat tgcatcaaaa
      301 ataatgttat actctatgtt tgtaatagct gttctctttt cagttaatca tacaagagct
      361 ttggacatat caaatttgga tataggtata atgctcttag cagttttatt catacaatct
      421 ttgtcaaaat taattttgta tatatatttt gataaaaagg gaatatgtaa ctaagtaata
      481 atatttatga aaacaaaaat taaaaaatat agagaatata taacttttaa ggagtgattt
      541 tatatgtcta aatcagaaac tggtgtaaga tcaaagctca tcttggaatt acttgtaata
      601 acttattttt ttatagctgt attagctact aaatatattt ttaaaggtcc aagcactgct
      661 tctcttccag ggttgagttg gttaatagtt tttggatgtt attcaattta taataaaaaa
      721 tataatgatt tagttgatga atcatctgaa tttatactat caaaggttaa taagattgca
      781 ataaaaataa tgatatattc tatagtaata atatctattt tctttgcaac accttataca
      841 agtaatataa atatatcaaa cctagataca ggtataatcc tcattgcagt tttatttata
      901 cagtctttat ctaaattagt gttgtatata tactttgata aaagggggat atgtaactaa
      961 gtggcaatat tgatgaaaac aaaaattaaa gaatacagag aaaaactcct tatgacgcaa
     1021 aatgaacttg ctaaattggt tggggtaaga cgtgagacta ttgtacactt agaaaatggg
     1081 aaatataatc cttctttgaa gttagctatg gatatcgcta aagtattcga tactacagta
     1141 gaaaatttgt ttgaatttat tgaagaagaa tagtggaaag tttataaaca tttataagat
     1201 ggataatgaa atatagttat ttaattatta agtttattaa aaatatttat ataatatgtg
     1261 attaaggtta gattataatt gttttaagat aataagaaga gtattataaa tgataaaaaa
     1321 tagtgaatat acagttaatt aataaacaat agataaaaat ttaaagttta aatgtaaaga
     1381 ggatgtataa ttttatgaaa tctaataaga cactgttata atcgctatgt aaggaaatca
     1441 tattagaatt ttctaagaaa acagttaaga atttggaagt aagtatgtgt tatttatata
     1501 cttctgaaac atataaacta ataagtgagg ttgtatcaga tatgtcttgt aagggtaatt
     1561 tcttcaggtt catcaactat cataactcta cagagggtat aagatatgct ttgtaagggt
     1621 aatatatgct tgattcaaga attagtgtta taaaatggat tagcatacca aaaggtctat
     1681 taaagatata cagttaaaaa gctatatagt aagttaggta aggttaataa tccaaacaat
     1741 gatttgatga ttaaactgtt tagagcctta gatacaacag taaaagaatt attttttaat
     1801 aaggagaagt aaaagataaa agaaaatcaa aacttaggca gttattgtag ttacaaaata
     1861 aaggatttcc ttatatatgg ataaataatc gagtatatat aataaaaaat atatgacctt
     1921 aattttgtta tgaaataact tcaaaactta ttttagcatg tttagattat ataaatcata
     1981 taaaaaaagt agaatgagtg aatacttatg acaataatct agtagagata tacatgaaat
     2041 tatatattga ctaagtacag ttattatttt ataatgaaaa taaagttatc atacagataa
     2101 ataaacggat cgttactgag atagatgtaa tcaggcgtga cctaaaaatt ctagtaaatg
     2161 cttaaagtat tattagtctt ttttaggtct ctttttgttt acagtggagg tagtttatgg
     2221 ttataaagaa agtatttaat aataatgttg tatttgccag agataattca ggacaagaag
     2281 taatattaat gggaaaagga attggattta ataaatttac taaagattta gttgaaatag
     2341 ataaaattga aaagaaattt atattcaata atgaggaatc attaagtgaa ttacattcac
     2401 tactagatag gataccttta aaagatgttg agcttgtaag tgaaattatt caaatgggaa
     2461 gagaagaatt gggtgataat cttaatgata gtatattaat cacattatca gaacatataa
     2521 gttatatgtt aaaaagaatg gatgaaggat tagtatttca aagcccgctt caatgggaaa
     2581 taagacaaat atatcctaat gaatataatt ttagtaagaa agctgtagaa tatttaagaa
     2641 aaaaattaga taaaaatatt cctgattctg aaatagcatt tattacgata cactttgcaa
     2701 atgctcagtt agaaacaaaa gatatgcaag aaactttatt gttaactaag ataattgata
     2761 atgtattaga tatcataaag tatcactatg gaatcgaaat aaatgaatat tcctttgact
     2821 acacaaggtt tataagtcat ttaagatgtt ttataaaaag gcaattaagt ggtgagaata
     2881 caaatgtaga cacatcattg ttagatataa taaaaatgaa atataaaagt gattacaatt
     2941 gtgcaaaaaa agtaaaaaac tttcttgaaa agacgtataa atggaaaatt agcgaaaatg
     3001 aattgttgta cttaacattg catttaaata gattatcaaa tataaagtaa atagtgtagg
     3061 attgttactg tttggcaggc atgacctaag ttggtataat tagtttttaa tatctaattg
     3121 tattgattta ggtctatttt tatacataaa attatgaaaa tggaggatag attgtatgaa
     3181 atttgaaaga gagtctgaag aaattataaa atatgtcgga ggaaaagaaa atattattag
     3241 tttagttcat tgtgcaacta ggctacgttt taagctgaaa gatacttcaa aaactaacaa
     3301 agaagagtta ttaaaaatga aggatgtact atctgtagta aatagtggag gacaatacca
     3361 agtggtaatt ggaaataaag taactgatta ctttgaaact attataaaaa aagcagtttt
     3421 acaaggacaa aatactgaag aacagggaga aaaagtttca atagtttcta aaatatttga
     3481 atcaatttca ggtgcatttt ctccattaat acctgcacta gctggagcag gaatgattaa
     3541 ggcactgctt acaatactaa ctactatgaa atggatgtct gatacaagta gcacttatgc
     3601 tatattatca gcagctggaa atgctgtatt ttacttttta ccaatattct taggtattac
     3661 cttagctata aaacttagtg caaatccata tgttggtgga gtgataggag cagctttgtt
     3721 agatcctaat tttacagctt taattggagc aaaaggtggt gctaactttt ttgggatgcc
     3781 agtaacagca attgattatt cttttggtgt attccctata tttgttgcta tatttattta
     3841 ttactatgtt gataagcttt taaagaagat tatattaaag gatttacaat tattcttagt
     3901 accgatgata tcattattag tcatggttcc tttaactgtg attttatttg gaccaatagc
     3961 aactaatgtt ggtgatataa tatctaatat ttttatggga ttatttgaat ttaacagagt
     4021 aattgcagga gctgcaatag gtatagctat gccattctta gcagtattag gactacactg
     4081 gggcttcaca ccaattactt tgcaaaactt ggctacaatg ggtggagacc caatagaagg
     4141 tgcagctgtt gcagcagtat ttgctcaaat aggtattgca ataggtttat ttatgaaggt
     4201 tagaaaatct aaaaaaaatt cagaattaaa ggtattagca ggttcaacag cagttacagg
     4261 aattttagca ggagtaacag aacctataat ttatggaatt atattaagat acagaagaat
     4321 gattccagta ttggctattg caggaggtat tggtggtggt atatgtggtg tatttggtgt
     4381 tacttgtaat gcatatgtat ttcataatat attctcaatg ccagtttata caccattcct
     4441 aggatatata ttaggaatag gaacagcatt agtgctagga gcagtattgt catatttctt
     4501 tggattgagt aaagaagaat gtgaagaagg taatgaagaa gttgcttttg atgaagatga
     4561 tggtatagag gagatattat ctccaataga aggcttacaa atagagttat ctgaagtaga
     4621 agatgaagtg tttgcatcag gaactatagg taatggcatg gcaataatac cttcaaaggg
     4681 agaagttgtg tctcctgtaa atggaacaat aacaactatt tttcctacaa agcatgcaat
     4741 aggaattacc tctgataaag gtgtagaaat attaatacac gtaggtctta acactactat
     4801 gttagatgga aagtattata ctactcatat gaatgcagga gataaagtta aaaaaggaga
     4861 taggattgtt tctttcgata aggataaaat agaaggtgaa gggtatagta ttgttacacc
     4921 aataatacta actaacacaa atgatttagc agatgtaata gttaaagaaa ataaacatat
     4981 taaaattgga acgcctgtaa taaaagttgt ttattaatta tactggagga gatatttcta
     5041 tgaaatctaa tcaagaattt ttatggggag gggcagttgc tgcccatcaa gttgaaggtg
     5101 gatataataa aggtggaaaa ggaataagta ttgcagatgt gatgactgca ggtactcata
     5161 ctatttcaag aaagataaca gatggagtaa ttgagggttt aaattatcct aatcatgaag
     5221 ccattaattt ttatgaaaat tataaagaag atattagatt atttgctgaa atgggatata
     5281 agtgttttag gacaagtatt gcttggacta gaatttttcc aaagggcgat gaatcaactc
     5341 caaatgaaga tggattgaaa ttttatgatg atttatttga tgaattatta aaatacaata
     5401 tagagccagt aataacttta agtcattttg aaatgcctta tcatttagta aaaaactatg
     5461 gtggatggag aaatagaaaa ttaatagatt tctttgtaaa cttctgtgaa gtggtaatga
     5521 atagatacaa ggataaagtt aagtactgga tgactttcaa tgaaataaat aatcaaagta
     5581 taactacaaa tcctatttat gcatttacta actcgggaat aatctatgaa gagcaggaag
     5641 acaaagaaga agttatgtat caagcagttc attatgaatt tgtagcaagt gcaaaggttg
     5701 tgaagatagg tcatgaaata aaccctgagt ttaaaattgg atgtatggtg gcagctatgc
     5761 caagttatcc ttattcttgc aatccagagg atatgattaa atttgtagaa tctaatagag
     5821 aacaacttat gtttactgat gtacatgtaa gaggtcatta tccaagacat acactaaaat
     5881 tatgggaaag aaaaaattat aatttagata ttacagagga agataaaaaa atcctaaaag
     5941 aaggaatagt tgattttatt ggatgtagtt attatttaac tactgtagta acagctgata
     6001 aaactatgaa aacaacaggt aatgactcgg caggaaaggc tgatgtagtt gaaaatccat
     6061 acttaaaaat ttctgattgg ggatggaata tagaccctgt aggtctaagg ttttacctta
     6121 atcaactata tgataagtat gaacttccta tattcattgt tgaaaatgga tttggagcag
     6181 aagatgtatt aaaatcagat aatactgtag atgatgatta tagaatagag tatttggcta
     6241 gtcatataag ggaaatgaaa aatgccattg aaattgatgg tgttgatgtt atagggtata
     6301 ctgtttgggg atgtatagat ccagtatcat ttacaacagg agagatgaaa aaaagatatg
     6361 gatttatata tgtagataaa aataatgatg gaagtgggac tcttaaaaga tataaaaaga
     6421 aatcttttga ttggtataaa aatgtaataa aatttaatgg agaaatttta taggtagata
     6481 aattttaata tttataagat tatgttaagg aaaccattaa aaattagatt aaaatcgtgg
     6541 gtatttaaat ccactaaaag atattgacta tttagtcaat atcttttttt attaaaaagt
     6601 aaatataaag aggtaaggtt aaaaattggg atatatatga tttatatagt atgatttaat
     6661 tagaaggagc tatattagta ttcatttgta tagttggaga ataaatgtca gtcttacctt
     6721 acgattttta taatagtcct atttaatttc tattgtaact aatatgcaag aatttaagat
     6781 agaagtatct atgacttatg gtgtattaat aattggtatc tgctatctag tatcttttta
     6841 atttttttga ttccaatata gtgctaacta cgatacgaat atctttaaat ttatagctaa
     6901 actatctaat tgtttattgg aaagagttaa gaaggctaaa gcataaaaca cttaaaaaga
     6961 gaagatgtat atgagaaata taattaacta tgatgcagac ttctaaaaat tatttagtga
     7021 aaaggaactt agagtgtata ataaaagttc cttttcacta aggaaaacga ttgttatttt
     7081 ctgatcttca ttaccctgat attatttaaa tataaacaac aacaaaatta acaagaagca
     7141 aattcaacct aaaatttaaa atatcgggag gtacaatatg ttaataaata tgaaggaaat
     7201 gctaaaagtg gcacaagaaa atcaatttgc tgtaccagca tttaatatag gaagtgggca
     7261 aatacttaaa gcagtagtac agagtgctaa cgaaaaaaat gcaccagtaa tacttgcaat
     7321 acacccaaat gagctaagtt ttctaggaga tagctttgta gctagttgca tagaagaagc
     7381 aaataagagt aaagtaccta tggtaataca cttagatcat ggggaaaata aagaacaaat
     7441 attaagggca ataagatgtg gatttacatc agtaatgatt gatggctcac atttaccata
     7501 tgaagagaat gtagctatat ctagggaaat agtagaaata gcaaaaggat taaatgtgtc
     7561 cgtagaagga gaacttggaa caataggaac aacaggaaca tcaagtgaag gtggtactga
     7621 tgaaataata tatacagatt caaaattagc taaggatttt gttgaaaaaa caggagtaga
     7681 cacactagct attgcaattg gtacagcaca tggtatatat ccaaaaggtt ttaaaccaga
     7741 gcttaaacta gatttattaa aagaaataag agaagtagta gatatacctt tagttcttca
     7801 tggtggttca tcaaatccag atgaagaaat agcacaagca gttaaactag gagtttgtaa
     7861 aataaatatt tcgagtgatg ttaagtcggc atattataag aagtgcagag aactattaga
     7921 acaaaaccca agcttatatg agccagatac tatatatcca ccatgtataa aatctgcaag
     7981 agaagttata gagtttaaaa tgaatctatt caatgctata gataagttaa agtgctttta
     8041 taaaaataaa aattaattat gaggtaacaa attaaaagga ggggagaatt tatgataggt
     8101 aaaatactac gaagcgattg tattgtacta gataaacaat tacaaacaaa aaaagatgta
     8161 attaattata tggcaaatca tttgtaccat ggaaattata taaataataa agaaaagttt
     8221 atagctgata ttaataaacg cgaaaaagaa tcatcaacta atatgggtaa tttaatagct
     8281 atacctcatg ctaagtcaaa tgtagtaaat caacctgtaa tgatgtattt aagaacagat
     8341 agagagatta cagataatga tggtcaaaaa cttaggtcat tttttatgat agcagttcca
     8401 gaatcaggtt cagatgaaca tcttaagatt atatctaaac tagctacata tctaatggaa
     8461 gaaaattttc ttaagcatct actaagtgca gaaactccat acgaaatatt aagtatattt
     8521 aataataaag ctgcagaatc aggagatttt agtaataatc aaaataaagg tcttatagtt
     8581 gggataacag gatgtccaac aggtatagct catactttta tggcaaaaaa agcattagag
     8641 gaagcagggc aagaacttgg atatgaagtt aaaatagaaa ctcatggagc tataggtgta
     8701 gaaaataagt tgactaaaga agatataaaa agagctgaat acgtaataat agcttgtgat
     8761 aaagaagttc aactgagtag atttatagga aagaaagtgc tacaaaagaa agtagtagat
     8821 gctaagaatg tttcattagc taaaaattta attataaacg ctgaaaatgg aatggctaaa
     8881 gaaattaaac caggtggaaa gactttagaa gattcaaaag aaatgcttca agggtcagag
     8941 aaaaagggtg tttataagca cttaatgagt ggtgtatctg aaatgattcc atttgtagtg
     9001 ataggtggaa ttggtatagc catagctttt atgtttggta tatttgcatc agacccaact
     9061 catgaaacat acaaccctat agctggattc tttggacaac ttggaggcga ttcagcattt
     9121 aagttattta taccgatact tgctggtttt atagctaaat caatagcagg aagacaagga
     9181 tttgctcctg ctatgatagc tggttttatg gcagttattg gaggttcagg gtttataggt
     9241 ggtatggtag ctggttttgc aacaggatat atagctttag gagtaatgaa gataacagaa
     9301 aatttaccag atgtattaaa aggagtaaat gctgtattga tatgtccact tttgattgca
     9361 tttacgagtg gagctataat gttctttgta gttaatacgc cagcatcatg gttaaatgaa
     9421 gctttacaat cttggttaca aagtatgtct ggaactaata aagtattatt aggtgcaata
     9481 ttagcaggga tgatggcatc agatatggga ggacctatta ataaaacagc ttcagctttt
     9541 ggattggcta tgttttcaag taatatattt gaaccatcag cagcattaat ggtaggagga
     9601 atggtacctc cattagcaat atcattggct atgttaatat tcaaaaataa gtttactaaa
     9661 gaagaaagaa agtcatctct accaactgct ataatggggg catctttcat aacagaaggc
     9721 gcaataccgt ttgcagctgc agacccatta agaatgattc cagcaaatat aattggttct
     9781 tgtataggtg gggcaatatg tatgagttta gatataactt taatggcacc acatggggga
     9841 atatttgtaa taccatttgc atgtagtgca ccagttgcat atatagcttg tatattgatt
     9901 ggtacagtaa taacagcatt gattataggg gtaacaaagc ctactataag gagtgaaaat
     9961 agtgaagttg aattacttgg tgcttaaatt ataaataata gtaaaaataa ttaatggaat
    10021 aacaacaagt atatagaata ataataagga tatttagaat cagttaacta cttctaaata
    10081 tccttaattt ttattttgaa attatattat ctattatatc aatttagtaa ggtgtatcat
    10141 aaattctttt atgatacaga agtatcttag taattttaca tagttagtaa atatgttaaa
    10201 atagtactta atataaacat agaaatatat aaacataggt agggagggct aagtatgctt
    10261 gtgtcgaggc aaattgaatt tcttaaattg atactaaacg aaaataaata tagacctata
    10321 aaatacttta aagataaatt aaatgtatct gataaaactt tacaaaaaga tttgaagatg
    10381 attgagaaat atttagaaac ttttaatata aaaattgata taaaaagagg ctatggaata
    10441 ttaatagaag atttagcaaa gaagaacata gacttaatta atagtttaaa tatacaatcg
    10501 aaggaaaata aatctttatc agtagagcag agaagaatgg agattttaaa gtatttatta
    10561 ctaaattcaa ataatataac ttcaataaat cagttagcag ataaatatta tgtaagtaaa
    10621 acttctatag taaatgattt taaatatata gaggcatgga taaaagaata caatttaaaa
    10681 cttaataaaa ctttagaagg aactaagatt ataggtaaag aagtggatat aaggaaatcg
    10741 attgccaaaa tgatggatga tttattagat gaaaattatg aacaacatga tatatctgag
    10801 ttaacaagat tagattctgc tacatttagt gcattgataa atttatttga tattgattct
    10861 attatatttg tagaaactat aattacagaa ttagaataca atttaggata tactataaat
    10921 cagccctact atataaattt aattacccat atacttatat gtttaaagag aatcgaagaa
    10981 ggtaatcaaa tagaatctca ggaagaaaga gaaatttctg tagataattt ggacgagtta
    11041 gtatataaaa atgtcactac acttatagat aaaatagaaa atagatacag agtaaaaata
    11101 acgaaagatg aacttatgta catatatatt tttttggtat catctggatt tagtagcgaa
    11161 agtaatagta gttacaatga tgaatcagat agattaagtg aaagtgaaaa agtttcatct
    11221 ataatgatag agaatatgtc acgattttta aatataaatc tcaaaaatga tgacttacta
    11281 cagaaaagtc tagcatctca tgtaaggcct atgttaaaca gattaagata tgatatccaa
    11341 ataaaaaacc cgttgttagg agagatacaa gagcgatttt cagatgtgct gggattatgt
    11401 atgatgacta taaatattat gacagagtat gataatttga agaatattag tatagatgaa
    11461 gtctcgtatc ttgccacata ttttcaagcg gcaatagaaa gaaatatgag tagtaaaaga
    11521 gtaatagtag tttgtcatac aggatatgga acatctcagc tccttgcagc cagattaaaa
    11581 agagaatttc ctgaatggac tatagttgat atagttccta tgcatcagtt gagtaagcga
    11641 aatttagatg atgtagactt tgtaatatct actgtaaact tagatttgaa agataaatta
    11701 catatagttg tatcagtact attaatggaa aatgatatta ataatataaa aaatgcttta
    11761 ctttctaaac ctaaaaaaag tatgaactta gatacaaact tattaggaat gtatttagaa
    11821 gataatatat attttaattt tgatgtagct cagatggaaa gtttaatagg tgtgaattta
    11881 gggagtaaat atgaatctat ttcattagga aaagatttaa atatatatat acataagcaa
    11941 agtaaattaa ataaagggat aatgaatatc aataatgtta ctagaacgat tgatatttat
    12001 ttagaaatta gtaatcaaag ttttttaaag aatattttaa tggaaatatc aaatttatat
    12061 aggcaaaaga actatataaa ctatataata gattgcaaga gcaaagagga tattaaaata
    12121 ttatttaaga ataatctatt ggagaaaaca aatggaaatt agagaaatat taacagaaga
    12181 aagcattatt ttagatttga aatcaactac aaaagaaggc ataatacagg aattagtaga
    12241 taaattatac gatacaggaa gtataacttt aaaaagtaag tttataaagg cattaaaaca
    12301 tagagaaaaa ataggctcta ctagtatagg ttatcaaatg gccatgccac atggaaaaac
    12361 aaaatatgta aagaaggcct ctgtagtaat aggtaaatct aaaaaaggtg tagattggcc
    12421 aagtatagat ggcttacctg taaaagtaat aattatgttt gctatgccac aatctggagg
    12481 caatgaagat atgatattaa tatctaaaat agcttacatt ttaggtggta aaaatatagg
    12541 tgaaagactt ttaaatgcac atacaaaaaa agaaatatta gatatatttt atgaagtgta
    12601 ggtattgtat aaattataaa tttgaggttt ttaaaataaa atgtgtattc ttttaaatag
    12661 tatagattaa tttttagaca ctagagagga gaggatatat atggcaatat atttagaaat
    12721 gcctaaatta atacatgaat ttccttttcg tagtctatca aatgaaggag aagtattaac
    12781 aacaccacac tggcataagg aaatagaaat attacttata acagaaggtg tagtaaatct
    12841 atttataaat gacaagccta tgcaattaaa aaaaggcgag atagtgataa taaaaggagg
    12901 agatattcat tatatactgc catcaccggg aagtgaaaga ttagtatttc aatttgatat
    12961 atcttttttt aatgatttaa ttttattaaa tgaggaaaaa gaaagcttaa ttaatttatt
    13021 cagtagtata aaaaaatgta gtagagattg gtcagcaaaa ataagaaagt cagttttcga
    13081 tatattgata gatatctaca atgaagacct atacaaaata gaaggatata gttatgcaat
    13141 aaaaggtaaa atgtataatt taattcctat attatataga cagattccta gagaaattga
    13201 agaaaataca gtagaatatg aaataaactc taaagagata ttagagagat tgaacaatat
    13261 attcaaatat gtagaaaaaa attataaaca acctataaaa ttagaagatg tgtcttatga
    13321 agtaggattt agtgtatatt attttactaa atttttcaaa aaaaatacag g
//
LOCUS       DAELJO010000098        13335 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELJO010000098 DAELJO010000000
VERSION     DAELJO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.098"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(188..712)
                     /locus_tag="KPB88_002337"
     CDS             complement(188..712)
                     /locus_tag="KPB88_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0054609.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGMDYSDVEKN"
     gene            complement(777..1529)
                     /locus_tag="KPB88_002338"
     CDS             complement(777..1529)
                     /locus_tag="KPB88_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0054610.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1560..2858)
                     /locus_tag="KPB88_002339"
     CDS             complement(1560..2858)
                     /locus_tag="KPB88_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG0054611.1"
                     /translation="MDSINFAKNLIDFICDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2875..5139)
                     /locus_tag="KPB88_002340"
     CDS             complement(2875..5139)
                     /locus_tag="KPB88_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG0054612.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5394..6212)
                     /locus_tag="KPB88_002341"
     CDS             complement(5394..6212)
                     /locus_tag="KPB88_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0054613.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="KPB88_002342"
     CDS             complement(6205..6903)
                     /gene="cbiQ"
                     /locus_tag="KPB88_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG0054614.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6906..7190)
                     /locus_tag="KPB88_002343"
     CDS             complement(6906..7190)
                     /locus_tag="KPB88_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0054615.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7180..7932)
                     /locus_tag="KPB88_002344"
     CDS             complement(7180..7932)
                     /locus_tag="KPB88_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG0054616.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8115..8307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8439..10211)
                     /locus_tag="KPB88_002345"
     CDS             complement(8439..10211)
                     /locus_tag="KPB88_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG0054617.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVRKYLTNRIRKGYNKDIILGLIQVILNTIVEEKAIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDEDNVKSVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKSSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLARILVEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10225..11154)
                     /locus_tag="KPB88_002346"
     CDS             complement(10225..11154)
                     /locus_tag="KPB88_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0054618.1"
                     /translation="MDIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDAYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11618..11770
                     /locus_tag="KPB88_002347"
     CDS             11618..11770
                     /locus_tag="KPB88_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054619.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNTDPPKGIIERINEWT
                     DKIGLD"
     gene            complement(12142..12750)
                     /locus_tag="KPB88_002348"
     CDS             complement(12142..12750)
                     /locus_tag="KPB88_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG0054620.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(12928..13044)
                     /gene="rrf"
                     /locus_tag="KPB88_002349"
     rRNA            complement(12928..13044)
                     /gene="rrf"
                     /locus_tag="KPB88_002349"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(13171..>13335)
                     /locus_tag="KPB88_002350"
     rRNA            complement(13171..>13335)
                     /locus_tag="KPB88_002350"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         4269 a         2411 c         1431 g         5224 t
ORIGIN      
        1 aaattttttt aataaaactt tattttttag ttaaaatcat acaatatata gatttccaca
       61 atacaaataa attaaaaagt gaaatgtatt tgtctaaaaa cataataaca tttcacttta
      121 tcctcatttt taagtaatat atatttttaa tttttattta taactaaatc tagtttttat
      181 ataaatttta gtttttttct acatctgagt aatccattcc aaaagtttct acagtaacag
      241 atttcataac ttgtggagtt tgtggcttat cagctgaatc tgttctaact gatgctattt
      301 catcaactgt atccatacct tctatcactc taccaaaacc tgcatactga ccatctaagt
      361 gtggtgagtt tttatgcatt atgaaaaatt gagaaccagc tgaattaggt gccatagttc
      421 tagccattga tatgactcct ctttcatgtt ttaaattgtt agtaaatcca ttaccactaa
      481 attcaccttt tattgagtga cctggaccac ccattcctgt accatttggg catcctcctt
      541 gtatcataaa tcctggtatt actctatgaa atattattcc attgtaatat ccttcattta
      601 ctaatgatac aaagttttta actgtatttg gagctatatt tggatataac tcaattttga
      661 tttcttttcc attttccatt tctatagtta ctataggatt tttattttcc ataatttatt
      721 accttccttt tcattagtta aattacataa ttacatttat tatattatac tctaaattaa
      781 agtctatcaa gtatattttt tcctatttca tatccttctt gataaatagt tttacaaaac
      841 tcatcatcaa agttaagcgt gtctttagga gcaaattgag tattaggtct aactactatt
      901 attttatctt catcatatac ttttttaata tcttctggca actcataatt atattttgtg
      961 gatattaaaa taattttatc cacattatcc ccaattaatg gttcaattaa tgcactccta
     1021 attaaacctc catctaattt gaaaccatca taaattcctt cttgcacatc tttcacaaat
     1081 tgttctttaa atccaagagt tgcgtttgga tttgatggta acaacccact gtatgaaatg
     1141 ctgataatgg cttcattacg aggcattttt gatacgtcaa caatcttttc tgatactact
     1201 ttattatcaa cttcaacata attaacataa aagttcattt cttctatatt actatcatct
     1261 agttccctta ggttgtctat agcaggagaa ttatccacag tattatttac tatttttaca
     1321 tttccattct ggatatcttt tatatttgtc cacattttct ccaagttatc aacattccct
     1381 gtgtacaaat aatatccatt tattgctcct attgatgtcc ctgaaatcga ataaaattta
     1441 tttattcctc catcatacaa ggctttaact actcctgctt gataagcccc ttttgctcct
     1501 ccaccttcta aacataatcc taatttcata gtctcatctc caaattgttt atcttatatt
     1561 tatatttcaa aaaacttact aaatgtttta tatatactat agtggtcctc aacatttcct
     1621 aattctctta tagagtgcat tgctaaaatt ggacttccca catccacaga tggtatatct
     1681 atatgagtag aagatattgg acctatagta cttcctcctc tttcatctga cctattaaca
     1741 aattcttgat actcaacatt agcttctcta catatatttt tataaatagc tatagaatat
     1801 ccatcactag tatatgcctg actagcattt atctttataa ccggaccttt tcccattatt
     1861 ggtttattag ttgggtcatg cttttctgga atgtttggat gaatagcatg agctaaatct
     1921 gatgatatca taaaagatga ataaatagac tcaaagaatc cctctctatc cttacccatt
     1981 gatatgcaaa ttctctccaa tatatttaac aacatgtttg aatctgcacc ttgtttagta
     2041 gagctaccaa cttcttcatt gtcaaataca gccattacat ttacaccatg ttgaccttta
     2101 gtattaacaa gagcattcaa actagcatgt accattgcaa gattatcttg tctcccaata
     2161 gatataaatt catcattagc ccctattaaa cttccttttt caaattcata taaaaataaa
     2221 tcaaaatcta taatatcgtc catatttaca tctaagttag atgctatttg ctttataaga
     2281 aaattatctt tttctaatgt atcatttaac atcccaacta aaggtaacat atctttttgc
     2341 ttattatatg agtgaccatc atttatactt ctatttaaat gtattgctag atttggtatt
     2401 atacaaattg gctttttaaa gtctataagc ctttcatttg gttttaatat actatcacct
     2461 tttaaaacta ctctaccagc tatcccaaga ggtctatcca accatgtact taatattgct
     2521 cctccatagc attctgtatt taacttcaaa taagattttt cagcttctat ttctgcattc
     2581 ggtttaatac taaatgtagg agaatctgaa tgagagccta ttattctaaa tccatctctt
     2641 tctatatctt ctgaatttac tacaaaagct actatagctg ataaattctt agtaacataa
     2701 tactttccac caactcttag attccatctt tgattcaaaa ctaattcttc aaatccattt
     2761 ttatctaaga tttccttagt actctcgact gcatgaaaag acgacggact atcacatatg
     2821 aagtctatta agtttttagc aaagtttata ctatccattc ctcatttacc tccattataa
     2881 aatatttagt gttgtatatt ctttatttat ttttttaatt ccctgttggt caaaagctat
     2941 agtaacatct gtaccaatta tagaaaccac tgtcccaacc ccaaatttag gatgatgtac
     3001 cttagaacca agttttatat catctggatt agtttcttta ttagaatctt ttattgtagc
     3061 attaacttta tctgcaactt tagatttgct tatagtactc atatactttt tagtatattt
     3121 atctaacata ttataacttg ctttagaata gcttagttct ttttttactt tgtacaatct
     3181 ttctatacat tcttctggca gttcttccat aaatcttgaa gctattgcaa catttgtttt
     3241 accatataat gttctcttct ctgtcaatga catatatagc tcttctttag ctctagttat
     3301 accaacataa caaagtcttc tctcttcttc tatttgtgaa tcactcatag attttatagc
     3361 tcttgatata ggaaataacc cttcttccat acctgttaaa aacacaacag ggaactctaa
     3421 acctttggat gtatgaattg tcataagcga aaccctatca ttgtcttctt catcatttga
     3481 ttcagatgta agtgctatac ttgttaggaa tgtctctaag tctttatttt cataagtatc
     3541 atcattacta ctttcaaact ccaaagctat agaaataaat tctctcaaat tatcaattct
     3601 gtcttgagct tcttcacctt tgcctgttaa atctttttca tttttatttc ttatctctac
     3661 aagctcgtcc atatatcctg tagtatccaa gactttttct ataagtttgc taacaggata
     3721 aacttctttt atagttctaa gagtccctat aatatcaaca aatccactta tacttgccct
     3781 tgccttagta gatatatctg aatttgtttc tatatcaatt aatactgagt atatactttc
     3841 ctgttttaag ttagctctat cttcaatttt ttctatagtt ctaagcccta tacctcttct
     3901 aggaacattt ataatccttt ttatagatat atcatcttgt ggattttgaa taactcttaa
     3961 atacgccaac aaatccttta tttcttttct ctcatagaat tttgtaccac catatatatt
     4021 gtatggaatt tgacttctat ttaaagcgtc ctctacaggt cttgcctgtg catttgctct
     4081 ataaagtact gcaaaatcct tataaggtct attttctttt ctagctattt gtgcaattga
     4141 atcagcaatg aaatcagcct cttctatttc actaccagtt aattgtatct ttataagttc
     4201 tccttctttt ttttcactcc aaagcttttt tctttttctt tctatattat tagatataac
     4261 tttattagct gcatctaata tcacttgtgt agacctataa ttttgttcca actttacaac
     4321 atgtacattg tcataatctt tctcaaattc aagtatgttt cttatatctg cacctctcca
     4381 tccataaata ctttggtcat catctccaac aacacaaata ttttgatgtt ctcttgctaa
     4441 taattttatc aattcatatt gagctttact tgtatcttgg tactcatcta ccattatgta
     4501 tctaaatcta cttctgtaat atgctaacac ttcatcatta gccttgaaaa gttctacagt
     4561 tttaagtatt aaatcatcaa agtcaagtgc actatttctt tttaaacggt cttgatatag
     4621 cgcataaata tctgcaatct taaccattct attatctgcc atattgatat ctttaaactg
     4681 ctttggagta taaagtttgt cttttgctcc tgaaattgca cttataactg cttttggttc
     4741 aaacacttta tcacttaaat ttaattcttt tagacagtct ttaaccaatg taacttggtc
     4801 tgctgaatca taaattacaa aacttctatt gtaaccaatc ttatttatat cttttcttag
     4861 tatacgaaca cagcaagaat ggaatgtact tatccacata tccttagttt caggacctat
     4921 attttgttca actctctctc tcatttcatt tgcagctttg tttgtaaatg ttatagccaa
     4981 tatatttggt gcttgtacac ctttatcttc tataagatat gctattcttg ttgttaatac
     5041 cttagtttta cctgaaccag caccagccag tataagtact ggaccttcag tcttttctac
     5101 tgcttctctt tgagcaggat ttaatgtatc taaattcata tttctcctcc tgttaacagc
     5161 cataaatata ttttaatcta aaatatgaat tacttcaaat tataattatg tattatatta
     5221 atagattatt tccaatatat tacaattaaa taacattttt ccatataatt tatttcattg
     5281 tttaataaaa aaaataacct tatgatttgg ggaatcataa ggcaccttta aggggtctag
     5341 gttaagggtt attatatata aatctatttt tttaaaatag gtttagggct taattattta
     5401 tttgtattaa gttctctttc cttccaatac ttatacaaat cttcttcttt cttaaataag
     5461 ggtaaattca tatttctatg tacttttact aaccatgggg attctaggcc agcttgtata
     5521 atattatttt catttataaa cacattttct gcctttcctt catcaatcaa aatcccctta
     5581 tccaatacat atatgtaatc acatatttca tacattaaat tcatatcatg actggatatt
     5641 actattttta tattattttt atttaatccc tttattatat ctacaataga cctagtactt
     5701 actgggtcta aaccagctgt aggttcatca agtaatacaa tttcattttc cattgctata
     5761 actgatgcta ttgccactct ttttttctgt ccatagctaa gaaaatgtac tggcctgtct
     5821 ataaaatcta ttccattaac agctactaga gctttgttta ttctctcttt aattgttttt
     5881 tcatccatac caatgtttct cattgcaaag gctatatcat catatacttt tgaataaaaa
     5941 atttgttttt ctgggtcttg gaagacaata cctacatttt ttctaaggtt gtataaactt
     6001 ctcttatcat attttaactt ttcttcttta aaaagaattt ctccagatgt aggctttaat
     6061 atacccatta aattcataaa taatgttgat tttcctgaac catttgaacc tattatacct
     6121 ataacatttc cctttgaaaa atccatatta atgtttaata gtgcattggt atttttttca
     6181 tattgatacg ttaaattatt tattttaaac atcattatcc cctactatgt gaaatttacc
     6241 atcatacaat tttatgtcta atgatataca catatcatca taccttgtca taagtctttt
     6301 gtacagcata cttccaagaa tacctaagga tttatatgaa gtttttaaat ttatatatcc
     6361 aaatcttagc tcttgtgact tgtatatttc tgaaaattct tctaaaaata tgaatataaa
     6421 cctatatatt aacattgata actcgacaaa agtgtcaggt aaatgaagtt ttttaaaaaa
     6481 gaatattaat tgattaaaag gtgttgtaag tatacaaaaa tacacacaag tcaaacaaga
     6541 catcgctcta aataacacat gaatagacat gtcaatagac gcctttgata ttcctacatt
     6601 aaaactaaaa aattcaaaac tatataataa atctgcttta ttaaaagata tatttacaag
     6661 agttattcct atactaagaa ataaaaagta cattggtatt ctaagtaatt ttaaataatt
     6721 ttttaaatct attccagcca cacaaacaac caaaatactc attaaaatca taattccact
     6781 taaaataaat atattttgta ttaccataga agcaatcaga aaaattactc ctatggctac
     6841 ttttttgttt ggatttactt tactcaatct atttgtatat gcatatttat caattataag
     6901 catgattaac ttctctttcc tttttgaaga cctaatatgt atcctattac accagcccct
     6961 aaagctgctt gtgcagagaa taataaactt tctatttctc cacttggtgg ctcatatgga
     7021 ctgctaaacc atggtttgta atttggattt atcttagtaa tttcactttc agcttgacca
     7081 tctgctcctc catattcagc acctgagttg acaagtaatg ggaatataat aagagctact
     7141 actaataaaa gtaataatat gttagttttt gtttttgttt tagcactcat tagctttaac
     7201 tcctttatca ttattttcta gtatgctttt ttctccgttt tctgtaatta aattatatac
     7261 aattacagta agaataccct ctgctattgc tattggtatt tgagtcataa agaaaattcc
     7321 taaaaattta attgctgaag ccattacccc acctgatggg tctgggaaag ccagtgctaa
     7381 ttgtattgaa gttattgtat atgtggctaa gtctcctatt gctgctgcaa gaaatactgg
     7441 aatagcatta tttccatctt ttttctttaa agccttaaat attaggaatg atacgattgg
     7501 tcctattatc gccattgaaa aagcattagc tcctaaagta gttattcctc catgtgctaa
     7561 tagcaatgct tggaatatta aaactatagt tccaagaaca aacattacgc ttggtccaaa
     7621 cattattgct ccaagaccta caccagtagg atgtgaacaa cttcctgtta ctgaaggtat
     7681 ttttagtgct gataatacaa acacaaatgc tccacataga gctaataata ctttcttttt
     7741 agggtcttgc tttactattt tacctattga ttttaatccg aataagaaaa atggtataaa
     7801 tataactccc catgctatag accattttac tggtaagtaa ccttccatta tgtgcattgc
     7861 atttgatgta actggagtta aaatgattaa cataagtgca gctataaccc ctaatttaat
     7921 attctgtttc atcttagtat ccctctctcc ctttctaata attaaaacat aataatacat
     7981 gtctgatttt atttaataaa ataaaaaagc ctaactcgat ggttaggctt ctaattttcc
     8041 acaatctaaa tacaaacaca taaaacaaac atatctgtat aattattata agaaactttc
     8101 tccatccccg aagaaattac tttaatattt taggcaggtc tcctgactta gcttcttcct
     8161 actcctcttc cttcccatga gatataaaaa atatcatcat agtggctaac aaaagtttcg
     8221 tctgcatcac agtagcgggg gctgtaaagg aatttaacct ttttccctat taatcttgct
     8281 taaagaacct ataatattat atatttaatt tttaaattat tcttaaatca ttttctatat
     8341 aaatcattgt ataccttttc tgtgaaaatt tctacatttt ttataaattt tattcatttt
     8401 tatattttta tgtatataaa ttcttttttg taagtttatt aatttctaat tatcttcttt
     8461 aaatacattg aaactacatc agaaaatcta gttatatctc ttgaatatat aaaatcattt
     8521 ccatatatta atctttcagc ttcaagttct gattcttttc ctgtaaatac tcctagtact
     8581 aatatatttt gctttctagc ttttctcact tcattggctg tatctctcaa accaactata
     8641 cccctataag ccttttctcc tcttaatgtt ctctcactac tttttcctat ctttacatca
     8701 tttggtcttc catcgctgag aactatcagg attttatttt cttcatctct atttattaga
     8761 ccactacata tagattttat tgccaaacca tctctattat taccagcaca aaaatattca
     8821 aatatatttt cactattttt tatactatca tcataatctt taaatctttt taatattgta
     8881 tagtctaaaa aactagaaaa tcccatcact ctatttggta tgccaacttc tgttagagca
     8941 tttgcaatta tgtaagcttg aattgccaca tttccttgat ttctactttg agagccactt
     9001 gcatccaata atatatcaat aacgtatttc cctttttcat tttctatatc tttataaaat
     9061 attttattat tattacttct accaactctc caagctctat ttgctaatat agtaccacaa
     9121 tcagaatata ctctactttt agcattttct tctactaaaa ttctggccag actttctttt
     9181 aatctcttta tttgctgtct atacattcta gcatgttctc taaattttcc taaattattt
     9241 tctttttgtc ttgttacata ttttagttga aatacattat tgcactcact tctaagaact
     9301 ccatctgtaa agtgtattct acaaccttca tgaacatttc tacaattttg tgtctcaatt
     9361 tttttaattt cacctttact caaataagtt tttccataat aatgttctat ttttgaatat
     9421 atcttatttg cagtgtcttc atcaacatat ataactcggt ctgctgaatt gctagatttg
     9481 gaatcatcaa aatcccctac accttctaca agcatggatg aagatatcga atctatatca
     9541 ctttcaataa tattttcttc ttcatcacta tagagttctt catacatgaa atcagcaaat
     9601 gtatcaaaat ttacttctac actttttaca ttatcttcat caataaggct attttcttct
     9661 ccatttaata tgtattcaaa ataagttaaa tatataacat ctaatgcttt taaaatttcc
     9721 ataatatctt gattggtatc tatatttctt atatcattaa ctattcttct agtcatacta
     9781 tctaatgctg ggtgcttgtc catactataa agtacaaatg tgtatttaat tttatccata
     9841 atattatcat tgtgtatttt ggtaaaacgt gaaacaatat ctttataagc atttcttctt
     9901 atgtcttcta tccctggtct atctttaatg gccttttctt caactattgt atttaatatt
     9961 acttgaatta gacccagtat aatatctttg ttgtatcctt ttcttattct atttgtaaga
    10021 tattttctaa ctatatccca atcaacatac tttcttctag cacctgcaat tattgcaaaa
    10081 tacagaccta tttcctttga agaataatct ttttctgata tgtctatact ctcattgtag
    10141 tcgccagcta ttgtccaaga taagttattg gctctatttt caaattcata atcatcatat
    10201 atccatttca taatctcacc tatattaaat atgacttatt ggaaacacat ctatactctc
    10261 ccatttatta ggtattctag ttttaattac atctccaacc atttctttct catatgcatc
    10321 aaaagtcttt cctgtaagcc ccatattaat tgcaagagtt ggtttcaatc ctcttcttat
    10381 agtcttaaga gatgccataa gaccacgtaa atcaatagct ttactagata tctcaccatt
    10441 ttgagacttc aattgtaaat ctaaaaatat tcctgcaaat tgtattaatt tttcttcatc
    10501 agcatctgaa aattcatttt ttaaaatcat cattaactta tcttcttcta taggaggaat
    10561 atctattacc ataaatctag ataccagtgc ttcattaagt tcttttgttc ctgcatattc
    10621 ataattcata gtcccaataa atcttgttgc tggatgtaga ttgactctct catacccagg
    10681 tacatcaatt attcttctat agtctaaagc tgaatgtacc acaactatag catcattttt
    10741 tgccatatta atttcatcaa ataccccaaa acctccatta atagcacact cataaactga
    10801 acctcttcta agtttaactt cattgtctat aaacgtatct gtacctataa gtgttgaact
    10861 gtctgtattt atatgaaatg atgtattcca ctgaggtcta ttaaatatct cacccaaatt
    10921 atcagcaagt aagttttttc ctgttgcctt tggtcctgat aataatatat tttcttcttc
    10981 taaaatagct gctatacaca tagataatat atcttttcca taaaaataag ttttagactt
    11041 tgttactcta tattctaaat ctttttctaa tccataataa tttctaaaat acatcacatc
    11101 ttcaataagc ttttcatcta ccccttgtgc ccttagatta tctattatat ccattattct
    11161 tacccctttt atataaatag tatttgtttc atttcttatc accattttag cataaaagga
    11221 tataagctag aattgatttt ttaaactaac ccaccttaaa caagacaatt tatcatttta
    11281 gcactacaaa aattaataag gacgacatga ttaagaagga ttcaataaca aatctacctt
    11341 tacataagaa tattacacta ctgtacaatg aaatcagcaa gtacaagata aattttgtag
    11401 atgtgacaat cacattatat atgtttatac gttcttacga ttaaccagaa taaagcatat
    11461 tacttactta tatttcttga ttttattcgt atacacatat ataatagtag aaaaaattat
    11521 gggaagtatc ataagtttgt tgatttgtgt ctgcctttct gcatttatac tgaaagggct
    11581 aaaatacaat gaaaaaaatt aaaataggta tcttaaaatg aaaaagataa aaaagctgat
    11641 tatactaatt gtatttttgt ttctttttgg tatgctacac tgttatactg aatggaaatt
    11701 caatactgac ccacctaaag ggataataga aagaataaat gagtggactg ataagattgg
    11761 actggactag taacttaagt taattatagt gaatgtaata ttagcaagtt attgtttcat
    11821 attttaatac tataacttta aatatttggt tttaatctgc caaacgaatg taaaaaaact
    11881 gtcacagagg caacatattt ttgaatattt ataaagcggt agttttgatt ttcaaagctg
    11941 ccgcattttc ttttatataa gcaaacttac caccaacaaa ttgtaaaaca tggtatattt
    12001 taaaaaaaag caccacagac ataagcgata aaagaaacgc cgagaaccta caaaaacgta
    12061 gtgtttatgc agatttacga cgccttctat tttctcaacc gtcttaatga ccttgtacaa
    12121 aagaaatttt tctaatattt tttataatct tatcttgtga tgttttttat gtataaatga
    12181 cttaataaca aatccaataa aaattatgga tattggtata attagaacta ggaatttata
    12241 atcattataa tgtgacatta ttcttaccca actctcagaa aaaatatatc ctatactgat
    12301 taatgaagta ttccatatgg ttattcccac gatagatgat actacaaaag ttataatatt
    12361 ttgacctgct atacctgcta taaaagatat gtatgttcta cataaaggga ttaatctaca
    12421 tacacaaaca gaaatagaag agtatttatt aaaataatct tgagatgcaa atattccttt
    12481 ctttgccttt ggacatatct ctattatttt atttatgata gatttacctc cccaatttcc
    12541 cactgtataa caaaatatcg aaccaagtat acctgcaatt atacttaaaa ttaaaattgt
    12601 caaaaatcca aaatgatttc ttgcagcaat tgctccacat aaaggtaaaa caacttcgct
    12661 aggtaatgga aaacaagcat actcaatcat aattaataca aatacagaga ttaatccata
    12721 ttgtaaaata aaactattaa ttacagacac ttacttatct atctcctttc ttatttaaag
    12781 gttttaaggg tacatatgct aaggattctc accctatatt taaagaatca atctctttaa
    12841 actattatat gtatttacac aaagcattta taacatactt taaaaatagt accttaaaat
    12901 aagaaactta ataaataaaa aaagattacg tggctacgtc ctactctccc aggcagtttc
    12961 ccaccaagta ccatcagcgc tagagagctt aacttctgtg ttcggaatgg gaacaggtgt
    13021 atcctctctg ctattgtaac cacataatct ttatgcttaa tatttctatt aagtttttaa
    13081 taagtttata gaacctagta atactagttt ctttagagtt aacactctaa aaactacaca
    13141 tatatcttaa tgaatgaaca tcaaataata ttggtcaagt cctcgaccta ttagtatcga
    13201 taagctaaat acattgctgc acttacacct tcgacctatc aaccagatag tctttctggg
    13261 gtcttaccct tgcggtggga aatcttatct tgaagttggc ttcgcgctta gatgctttca
    13321 gcgcttatcc attcc
//
LOCUS       DAELJO010000099        13318 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELJO010000099 DAELJO010000000
VERSION     DAELJO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.099"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            114..611
                     /locus_tag="KPB88_002351"
     CDS             114..611
                     /locus_tag="KPB88_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0054621.1"
                     /translation="MNYEIVEINEKIVIGISKVTTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            1355..1717
                     /locus_tag="KPB88_002352"
     CDS             1355..1717
                     /locus_tag="KPB88_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0054622.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            1782..2399
                     /locus_tag="KPB88_002353"
     CDS             1782..2399
                     /locus_tag="KPB88_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG0054623.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            2396..2881
                     /locus_tag="KPB88_002354"
     CDS             2396..2881
                     /locus_tag="KPB88_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG0054624.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2924..3442
                     /locus_tag="KPB88_002355"
     CDS             2924..3442
                     /locus_tag="KPB88_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG0054625.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            3578..4537
                     /locus_tag="KPB88_002356"
     CDS             3578..4537
                     /locus_tag="KPB88_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0054626.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            4563..6266
                     /locus_tag="KPB88_002357"
     CDS             4563..6266
                     /locus_tag="KPB88_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG0054627.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDGIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            6563..8665
                     /locus_tag="KPB88_002358"
     CDS             6563..8665
                     /locus_tag="KPB88_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG0054628.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPNDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            8678..9118
                     /locus_tag="KPB88_002359"
     CDS             8678..9118
                     /locus_tag="KPB88_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG0054629.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            9154..9957
                     /locus_tag="KPB88_002360"
     CDS             9154..9957
                     /locus_tag="KPB88_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG0054630.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNAPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9997..10593
                     /locus_tag="KPB88_002361"
     CDS             9997..10593
                     /locus_tag="KPB88_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG0054631.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAM
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            10699..11562
                     /locus_tag="KPB88_002362"
     CDS             10699..11562
                     /locus_tag="KPB88_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG0054632.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            11629..12618
                     /locus_tag="KPB88_002363"
     CDS             11629..12618
                     /locus_tag="KPB88_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG0054633.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            12710..>13318
                     /locus_tag="KPB88_002364"
     CDS             12710..>13318
                     /locus_tag="KPB88_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054634.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAKYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5401 a         1278 c         2492 g         4147 t
ORIGIN      
        1 tcactgagtt taattatatt aaaaatatga cataaaggtg tcgtatatta taaattataa
       61 ttgaattagt taaaaataaa atatattaat aaaaacaaag tgaggtataa agtatgaatt
      121 atgaaatagt agagataaat gagaaaatag ttataggaat atcgaaggtg actacaaata
      181 aagatggaca agcggtaaat gatataggtg aattgtggaa aaaatttatg ggcaaaggga
      241 tatataatgc tataaagggt aaaaagaatg acaagacaat aggtctatat acagattacc
      301 agggtgattt tacaagtccg tatagctttg ttgcatgttg tgaagtaaat agtaattctg
      361 ataaagagaa gaatttagaa gaattaaaca ctaataatac tgttggagaa agtattatta
      421 gtaaagttat accagcaggt aagtatgcaa aatttgttat agtaggaggt caaaaagaag
      481 taggagattt ttggtttaag ttttggcaaa tggattttga taggacttat ataagtgatt
      541 ttgaagagta tcaatgtaat acttttgata caaagaaaca agaagttcat atttacatag
      601 gaataaagta gatttgttat tactatattg aatgaagtat tgagtatagt acatttatta
      661 tagatagttt tattatttat aactataagc agtaaatctg taaaagattt actgcttttt
      721 tagtaattaa tattatcagt atcactttga atcttcagac atattcttca tttaatattg
      781 tttattgata aatgatactt gtaaatataa tgatttgtta tttaatttaa aatatgaaaa
      841 aaagtattat aaacacatta gaatacttct ggatttatga gtatacaatt tgaaaaatat
      901 aaaaaataat gtataaagct aaatctaatt taatcgataa tacatataga gaaatataat
      961 taagtaaata aacaactatc tattagatat tgagaaagtc aaatcctata tgaatctaca
     1021 gaaatgattt taatgaatct gaaaaatttt tttatacaaa cataaaaaag gtataaagtt
     1081 gttttattat aatttaatta cttttgataa ctttttatgt tttatacaat tatattttta
     1141 ataactcgtc aaaacgccaa acatatatta caatagatat ataagttttt ttgttgacaa
     1201 attatgttaa gagaattaag tataatatgg cataaataat tacttttatt tatcaatgaa
     1261 atgtatttat tgtgtagcga aaaatagtca gatagtatag acaataactg aagataaaag
     1321 cgtttattgt tgtacatatg gaggagttag ttttatggaa aataaaattt tgatagtaga
     1381 tgatgctatt tttatgagaa tgataattaa agaagctctg agtaaaagtg gctataataa
     1441 tttaatagaa gcaaatgatg gagaagaagc gtgtaatata tttaaatatg aaaagcctga
     1501 tttgatgttg cttgatatta caatgccaaa aaaagatggt ttagaggtgc taagagaaat
     1561 aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct gcaataggac aagagaatat
     1621 gattgtagaa gccataaaac ttggagcgtt agattttata gtgaagccct tcaaaacaga
     1681 aacattagtt aaagttgtaa ataatgtttt aaaataaaag tgtaaatata cactgataca
     1741 atgaaaagca aataaatgca ataataaagg gaggggttta tattataaat tatttagaac
     1801 ttgatgatgt tcatatagat gctcttagag aaattggtaa tataggctct gggaatgcaa
     1861 ttactgcact tgcatccatg ataaatagca atgtagaagt attaatacct atggtcaaga
     1921 tacttgaata taatgaagcg acaaatttgc ttggtggtcc agaaaacaaa gtagtatgta
     1981 ttttactcga tatgaaaggt gatatcaatg gaatgtttat gtttttgttg gatgaatcaa
     2041 taactcaact tatgctaagt tctcttttta ataaagaaga agctttttta gatgagattg
     2101 aggcaattga aatttcagca attaaggaaa taggaaatat tatggcaagt tcatatgtaa
     2161 atgctattgc atctatgtta aacatgacta taagtgtttc aattccagat atatgtattg
     2221 atatggttgg agctgttcta aatgtaccta tgataagatt ttcagatgtt ggtgacaaag
     2281 tcttatttat agaaaataaa tttaaaatga gtgataatta ttttactagt cacattttga
     2341 tgattccaga aatgagctca cttagagaaa tactcgtgag gttgggatta gaagtatgaa
     2401 tagagagata gtagtgaaca ttgcagatat gaagatagcc tatagaccaa atgttttagt
     2461 tacttatgca cttggttctt gtgtaggagt atgtttgata gataaagtag ctggaattgg
     2521 tggaatgtta cacgttatgt tgccatatag taaagatgct attaacattg aaaataagtg
     2581 taaatttgct gacacaggaa taaatgagct cattaaatct atggagaata taggtgcaaa
     2641 tagaatatat ataaaagcaa aaattgctgg aggtgctcag atgtttttag gaagtagcaa
     2701 ttcagtaatt gcaagtatag gacataggaa tgttgaggca gtaaaaaaaa ctcttttagg
     2761 attaaatatt ccaatattag ctgaggacac aggtatgaat tatggtagaa caatacgttt
     2821 ttacactgag acgggtgaat tacttgtaga ttcaataaat aaaggtaaga agaaattata
     2881 atatatattt catagaaagt gtattttagg agggaggtat taaatgagat ttccagatga
     2941 cccatattta gattatggac taattgatga aggaattaag tatctaaagt ttaaagtaga
     3001 tgagcaagat tttggggttg aacttgaaaa ggtaatagaa attactggaa atcaagaaat
     3061 tatatttatt ccagaactac ctacatattc taaaggcata ataaatctaa gaggaaaaat
     3121 cattccgata atagatatgc gtttgagact taaaaaagat gaattaatga ctacaaattg
     3181 catgtatata gtagtaactg aaataaagga cttggtagtt ggatttgtgg ttgatagagt
     3241 tgatgatata gtaagcttag aaaaaagtaa aatatcacct ccaccaagag ttactataga
     3301 atattcggat tattttgtaa gtggagttgg agaattagaa ggtaaaataa ttactttact
     3361 agatttagag aaacttttga cagataaaga tgaagtttta atagatgaat taatcaatgg
     3421 gtttgattgc actatcatgt aatataggga tgtagttgga gtgttattat aaaaagattg
     3481 tgatgagaac atttaagaat atagtggtat aattattaag tataaaaatt ttaaatcatt
     3541 aaaatttgaa tatattatgt ttaagagagg tttgtatatg aaaaataagc aacagggaac
     3601 aattttagtc gttgatgata gtgttctgat gtgtgactta atagataaaa cacttacaaa
     3661 agataatttt aaggttaaaa aagcatttaa tgcaatagag gcaaaagaat ttattgaaga
     3721 atttatacca gacattattt tgcttgatat aattttacca gataactctg gttttgagtt
     3781 ttgtaaggaa gtaaaatcta atgctcgtac agcagatata cctataattt ttataacttc
     3841 aaaaaataca gatacagaca tagtaaaagg atttggtgta ggagcaattg actatatggt
     3901 gaaacctttt agtatgacag aattaaaagc tcgtgttata gctcatcttg aagtaaagaa
     3961 atctcaagat aaactaaaaa agataaataa tgaactggaa tcttctcttg aaaaattgaa
     4021 tagactcgtt gtaagagatt atcttacagg tctttacaat aggcgacata taattaatca
     4081 gcttatggag caaagaagaa ttaataaata tagtgaaaca tcaatatccc ttatccttgg
     4141 tgatattgat gactttaagg ttatcaatga tacttatggg catgaagcag gggatttcgt
     4201 gttaacagta atatcaagta taataaaaaa gaattgtaga caaattgata ctgtatcaag
     4261 atggggtgga gaagagtttt tgataatgct cacaaatact ccaatagaag gtgctaagat
     4321 actttcagaa agaatccgta aagagataaa ggagtttgat ttcaactata aagaacataa
     4381 aataaagtgc actattacat taggagtagt agaagtaaac tcaaatatat cattagaaga
     4441 aaacatatcc ttagcagata aagcaatgta tgagggtaaa aacttaggaa aagattgtag
     4501 cgtagtgagt acaatgaaag gattaaaaaa gatataaact tactttatga aggagtacag
     4561 atatgaaaag agaagtaaag ctttctaaat tagcaattgt attgttgatt attatagtat
     4621 tggctgttct agcagggatt ttttcttcta tttcatctta taaagcattt gaaaaagtag
     4681 aaaaaagtat taatgatatg aataatttga ggactcaaag tcagtatata aaagatacaa
     4741 ctagagagat aagtgatgta ttaagaagat atgtatttac aggagaaaaa aagaataaag
     4801 atatttatga aaaagaatta tatggaaaaa caagagaatc agctttaaac aaattagaaa
     4861 aaattggtct tacaaagagc gagtcaaata aaatcttaga agcaaaagaa atatcagatt
     4921 ctttagtacc aacagaacaa agtgcaataa aggcagtcga aagtaatgat aagagtaaag
     4981 ctcaaagtat aatttttggt agtgactata taaactccca agagaaaatg gattctttaa
     5041 taaatgaatt tcaagaggat atagttaccc gttcaaattc caaaattgta gaagtaaaaa
     5101 aagaattaca aagtctaata ttaagaacaa tatttatact ttccctttta ataatattga
     5161 caataataga atatacagta ataaaattaa aaatagtgat tcctattaat aaagtagaaa
     5221 aacacttttc aagaattgca tctggggact taagaacaag tatagacgtt gaagaaagta
     5281 aatctgaaat agggctatta gtgagttctg tcaaaaagat gcaaagtatg cttttaaatt
     5341 acatagacct tatagacagt acattaacta gtattgcaaa aggagattta agagtagaga
     5401 tagaccaaga atttataggt gattttaaat caattaaaat atctttagaa agtataataa
     5461 actcttttaa tggtgatttt tctgaaataa atttatcagc agaacaagtt gcaagtgctt
     5521 cagaacaagt tgcagcaggt gcacaagctc tttcacaggg tgctactgaa caagctagtt
     5581 cagtagaaga attagcttcc acgattaatg agatatcaga tggaattaag aagtgtgcag
     5641 atagtgctag tcttgcatgt gaggtatctg accaatctgc atcacaagtt ggtttcggaa
     5701 gtagatgtat ggatgatatg atgacatcaa tgggtgaaat aagtgaaaaa tcgagtgaaa
     5761 tatcaaaaat aatcaaaaca atagatggga tagcattcca aactaatatt cttgcattaa
     5821 atgcagctgt tgaagcagca agagcaggtc aatatggtag aggatttgca gttgtagcag
     5881 atgaagttag gtctttggct ggtaaaagtg caaaagcagc tcaaaataca gctattctca
     5941 tagaagaagc tattaaaaca gtagaaaatg gtgtcagaat tgctaaagag actgcaaatg
     6001 cattggactt ggtagtaaat ggtgtagaaa aaactactaa ttatattaat ggtatttctt
     6061 cggctgtaca tagtcaagaa gtagcaatta cacagatttt atctggaata gagcaaattt
     6121 ccatagtagt ccaaatgaac tcagcaacag cagaggaaag tgcagcagcc tcagaagaga
     6181 tgtctgctca agctcaaatt ttaaaagaat tagtagaaaa atttcatcta aaggaaagtg
     6241 aagtaagaga atcatcatgg gtttaaatta tgaggtgaat attgaagtaa gagaatcatc
     6301 atgggtttaa attatgaggt gaatgttgaa gtaagagaat tatcatgtat ttaaattatg
     6361 aggtgaatgt tgaagtaaga gaattatcat gtatttaaat tatgagatgt atattgaaat
     6421 aagaaaatga ttgtgtattt aaattatgag gtacatatag tgagataaga gaatcatcaa
     6481 ggatttaaat tatgagatgc atatataata gaaaattatt tgtaggtcgt aggtgataaa
     6541 tgtgaattat attgaaccga gtatgagaca aatgttagat atgtttatat ttgagacaag
     6601 tttattactc gaacaattag atgaaattat gattcaaata gaaaaagaga aagtatttac
     6661 tcataacaac attaatgaaa cttttagaat aatgcatacg ataaaaggtt cagcatctat
     6721 gatggggtta gacaatatat ctgctttagc acatagtgta gaagacttgt tttatgctat
     6781 acgtgaaggt aaaatagaag ttttagacaa tgacctatta tttgatttag tctttcaatc
     6841 attagatttt ataagaagag atatagatga gttacaatca agaggttgta tatcttctga
     6901 ttactctaat tttattgaag gaattgaaga gtatgtgagt actttggaat tatcaagttc
     6961 tatgaaatca aaggataatg caaatttaga aagtacagac gaaaaagaag taatagattc
     7021 aaattttgaa tacaaagaag actctgatgt agtaaaaata atctttgaac aaggttgcat
     7081 gatggaaaat gtgagagctt tcatgcttgt atttgaactt agagattact gtgaatttat
     7141 agatttttat ccaaatgatg tagaaacaaa tgcagagtct acagagtata ttaaaagtta
     7201 tgggttttat atacaatttg tagctaaaga ggataaggca atagtttata aaactataga
     7261 agataatctc tatgtaaaag aatacataaa tattagtaaa aaagaaatag aaatgaaaag
     7321 tacatcaaac aatgatgcag tagaaaataa attaaaagat aataataaag taagtaaaag
     7381 ttatcaaaat aactatacag aagatgagat acaagaagat gttttaaatg aaaaaactga
     7441 tatggaaaaa gatagtctaa gtgaagaaaa tgaacattta ccaataagta agcagagtat
     7501 tatgagtgta aatttagaaa aattggactt acttcaaaat atagttggag aaattgtaat
     7561 tatggaatca atggttgtaa atagcgtaaa ttctacaggt gttgatatag ataatttcaa
     7621 taaggcaaca agacaattac acaagcttac agatgaattg caagatatag taatgtctat
     7681 gcgtatgatt caaattgctg gtgtatttca aaaaatgcat agaattgtac gagatatgaa
     7741 taaaaaatta gataaaaatg tagagttaat aactatagga gcagagacag aggtagataa
     7801 gagtgtaatt gataatttag cagaaccttt tatgcacctt ataagaaatg ctatggacca
     7861 tgcaatagag cctattgagg aacgtatagc gaaaggtaaa gaagaagtgg gaaagatagt
     7921 tttatctgct gaaaatctag gcggagaagc tgtgataagt atttcagatg atggtagagg
     7981 cattatgaga gataagattt tggcaaaagc taaggaacaa ggtcttctta aacatcaaag
     8041 taatgagtat actgatgacg aggtcaattc tttgattatg actccaggtt tttctacaaa
     8101 agaatctata actgaatttt ctggaagagg agtaggcatg gacattgttc gtaaaagtat
     8161 tgaaaaagtt ggaggttcaa ttgaagtaaa aagtaaacag gatgaaggga caacctttac
     8221 tataaaaata ccattaactc tttctatagt agagggtatg aagtttaaag ttggagattc
     8281 actatttact ttaccaatat catccataag aagaacattt aaacttacag acttaaatca
     8341 aataattaca gaaggaaata agacagagtt aattacaata tatggagcat gtattcccat
     8401 tataaggtta cataaaatat ataatattca aacaaaagta accaatctga tgcatggtaa
     8461 tatgataatt attgaaaatg agcataaagc agtatgcatt tttgttgatg aaataatagg
     8521 taatcagcaa gttgttgtga aaccatttcc agtttatttt aaccgattta atataaaaaa
     8581 taagggttta tcgggatgta caatacttgg ggatggaagt ataagtttaa ttatagatgc
     8641 agatagcatt atagaaatat attaggggat ttaaagtatg gctaaagatg aaattattaa
     8701 acaagtatta acattttatg tcaatgatgt tatttatgga atagaattag aaaatgtgat
     8761 tgaaaccata agatttcaat caataacgta tgtaccatgt cttccaacat atataagtgg
     8821 agttattaat ttgagaggaa gaataatacc agttattaat atgcatataa agtacaatct
     8881 tcctaaagct gattataatg agagaacttg tataataatt acaaaggttg atgagtatca
     8941 agttggaatt attgtagata aagttgttga tgttattcat atagaaaatc taaatttact
     9001 agagacgact aattctaaca acactaatat taataaagat ataaaagaga ttgcgaaagt
     9061 tgaagataat tctatattaa ttttagatat tcgaaagttt ttgattaata gcatatagtt
     9121 agttctgata aattagcagg aggaagtttt tgcatgataa aactcacaga tgaagaattt
     9181 atagttcttg taaaccatgt aaaaaaagaa tatggtattg atttaagtaa aaaaagagcg
     9241 ttaattgaag gtcgcttata caatacaatg atagagaaaa aacttagttc ttttagtcaa
     9301 tatatgaatc tattatttaa agataagaca ggaaatgaag ctataaacct aataaataga
     9361 ttgagtacta accacacatt ttttatgaga gaacctcaac attttgagtt tatacagaat
     9421 agtatacttc ctttttggga agaaaataat aaaataagaa acataaatat atggagtgca
     9481 ggatgttcta gtggagaaga agcgtattca atagctatga ctttggatga ttattttgga
     9541 tataataaag agttgtggaa cattaaaatc atagccacag atatttcaac taactcactt
     9601 gaaaaagcta aaagaggtat atacatagaa tcaaatgtta acaatgctcc tgagttatgg
     9661 aagaagaagt attttataga caacaaggat ggtacattta aaatttgtga taagattaga
     9721 aaaagtgtaa tttttaagcc gtttaattta atgaatgatt tctcatatca acattttgat
     9781 ttgatttttt gtagaaatgt tatgatttat tttgatttaa aaacaagaga agaacttata
     9841 aataaatttt ataatgctac caaaaaagat agttttttgt ttgtaggaca tgcagaagta
     9901 atcaacagaa gtagtactaa atacaaatat gtaaaacctg ctatatataa acgataaaaa
     9961 ggtatttttg atatatgatt ttttggaggc ggtaatgtgg aacgttttaa taatatggtt
    10021 attgctatag gcgcatctgt aggtggtaca gaggcaatac ttgaaattat aaaagaccta
    10081 ccaaaatcaa caccagggat tgttgtagtt caacatatgc cagctatgtt tacttatatg
    10141 tatgcacaac ggcttgacaa acagtgtatt atgaatgtaa gagaagctaa gaataatgat
    10201 agagtagaac aaggaaatgt gttaattgca cctgggggtt accagatgaa attgtgtacg
    10261 gataaacaag gatattatgt aacttgtgaa aaaggagaaa gaataagtgg tcactgtcca
    10321 tcagtagacg ttttgtttga ttcagtagca gaagtagcag gtaaaaattc aataggaata
    10381 atacttacag gtatgggtta tgatggagca agtggacttt taaaaatgaa agaaaatggt
    10441 tcatttacaa ttggacagga tgaaaattct tgtatagtat atggaatgcc aatggtagct
    10501 tttgatagag gttctgttat gttacaatta cctcttggtg aaatttcaaa ctgtttgata
    10561 agacatatta atattttaaa acaaaaagat taatttttta taaataaaaa ttaatctttt
    10621 attaaattat atggataaaa aaccctagaa actccaaata taataagaga gatttatatt
    10681 taggaggaaa atgataatat gaaatttttt aaagtcgata caaaagctaa agttttaaga
    10741 tatgtatttg ttttaacatt gactataatg gctgttttgg caattaaaca tgactataaa
    10801 tataataatt taaggaaaga tgcttcaatt aatttaaata gtaataagga agcagtgact
    10861 gttatgagta accagtcatt accaaagggt aatacaaata gtaaacaaac tttatcagta
    10921 agtacaatag gaagtaaaac aatagtgaaa aatgatgagt atttagaaag tactataaac
    10981 atgcctgtaa taacaaattc aaataaaatt gttgaaaggt ctacaaatga taagataaaa
    11041 aatgacatat ttgaatttta taataaatca tataaagaag caaagcagta tcttaaggat
    11101 aatcctgacg agaaaaataa gtttgttgca aatgtagact ttgaacttaa aaagaatact
    11161 gattctgctc ttagtataaa agtgagatat tatacttata gtggtggagc acatggattt
    11221 tatcaagata tagcatataa tgtggatatg agaactggaa aatttttaga attaatggac
    11281 ttgtttaagg ataatactaa atataaagaa gttatagatg aagaaattaa aagacaaata
    11341 gctgaacttg aaaaaaagga tgaagaaaat atcggaatat ataattttaa aggtataaaa
    11401 gagaatcaaa acttttactt gcaagatgag aatctagttg tatattttga tttatatgat
    11461 ataactccat atgcagcagg aataccagaa ttttcaataa ataaagcgtt gataagttct
    11521 atgctaaaac cagagtatgt agatttattt gatttgaaat agaagattaa attaaatgtt
    11581 atgctataat atttagcata gagacttatt aaaattgggg gtataaaaat gctactttta
    11641 aaaaaagacg acataaaaaa agtatttaca atgagagatg caattgaagc tgataaggaa
    11701 gcttttagaa tttattgtga aggaaagagt gtaaatcctt tgagaaccaa tatatctgtt
    11761 ccaagtcaag atgctagtat gttatttatg ccaggatacg tagaagaatt aggctgtggg
    11821 ggaataaaaa ttgtatctgt atttcctaaa aatgctcaaa aaggcaaacc agtaattcca
    11881 gcaacagtac tattattaga tggagaaact ggagaagtta gtgcagtatt ggatggtact
    11941 tatgtaactc agattcgtac tggagcagcc tctggagcag ctatagatgt attggctaaa
    12001 aaagattcta agattggagc tttgataggt ctaggaggac aaggtgagcc acaacttgaa
    12061 gccatgatag aagctagaaa tttggatgag gtaagagttt ttagtagaaa caaggaaagt
    12121 agagaaaaat ttgcagaaga aatgaactta aaattaagta aatataatac taaaatagtt
    12181 gcagttgaat catctgatga agccatagat aacgcagatg tgatagtttt agctactcca
    12241 tcaaaacaac cagtacttaa tggaaactta gtgaaaaaag gagctttaat aagtgcagta
    12301 ggttcatata tgccagatat gcaagagttg tgcccaaact gtttaactag agcgtctaag
    12361 atattttttg aatcaacaga tgcagtttta tctgagtcag gagatatatt aattccatta
    12421 aaagaaggta agataagtaa agatgatttt agtggtgatt taggaaatgt tataaacgga
    12481 actattcctg gaagagaaga tgatgatgag ataattgtat ttaaaactgt tgggataggt
    12541 gtacaagatg tcgtaacagc aaaaagaata tacgataaag caaaagaaaa cggtattgga
    12601 atagactgga agtggtaaaa tattcaaaat aattctatag ttagacaaat ttatgttata
    12661 atgttgataa ataaattttt tattatttta attgagggag gatgtttgta tgaaaatggt
    12721 aaaaaagtta atattatcta cgattatggc tctagtttta gttctaacta tagggtgttc
    12781 taatgcagaa gaaaaagtaa agtcgaattt tgaagctaat ttagctgcat tagaaatgta
    12841 taagtatgat gaaaaaggaa tttctgatgc attaacagaa gctaaatata aacagattca
    12901 aacttataac agcaaggaca aggtttcaat gattgctcaa ttgacacctc tttatggtaa
    12961 agaaaatgct gaaaagatat acgaaacaat tgttaaatta attcaaggtg ttgaaaagac
    13021 agtaacagtg gaatcttcta gtaaggataa gataactttg aaagttgtaa gtaaagcttt
    13081 agatactagt aaagtaatga gtgaagcgaa cactttagta aaatcttatg taactgatga
    13141 aaagaattac gataaaaaag aaaaagaagt tcaagatgat atgacaaaaa tgattataga
    13201 cctttacaat aagaaaccaa ctaaggaagt tacaactatg attacatata ctaaaaaaga
    13261 tggtaaatgg caaccaccta gtaatataca aaagttatgt gaaggaatat atacaatt
//
LOCUS       DAELJO010000100        13315 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELJO010000100 DAELJO010000000
VERSION     DAELJO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.100"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..591)
                     /locus_tag="KPB88_002365"
     CDS             complement(<1..591)
                     /locus_tag="KPB88_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0054635.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="KPB88_002366"
     CDS             complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="KPB88_002366"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0054636.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKLLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2761..3299)
                     /locus_tag="KPB88_002367"
                     /pseudo
     CDS             complement(2761..3299)
                     /locus_tag="KPB88_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3376..5181)
                     /locus_tag="KPB88_002368"
     CDS             complement(3376..5181)
                     /locus_tag="KPB88_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054637.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5577..5954)
                     /locus_tag="KPB88_002369"
     CDS             complement(5577..5954)
                     /locus_tag="KPB88_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0054638.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6859..7344
                     /locus_tag="KPB88_002370"
     CDS             6859..7344
                     /locus_tag="KPB88_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054639.1"
                     /translation="MSKSELTAETTEEMLKVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7544..8083)
                     /locus_tag="KPB88_002371"
     CDS             complement(7544..8083)
                     /locus_tag="KPB88_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0054640.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8076..8489)
                     /locus_tag="KPB88_002372"
     CDS             complement(8076..8489)
                     /locus_tag="KPB88_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0054641.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8525..9646)
                     /locus_tag="KPB88_002373"
     CDS             complement(8525..9646)
                     /locus_tag="KPB88_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0054642.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNIEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10245..11897
                     /locus_tag="KPB88_002374"
     CDS             10245..11897
                     /locus_tag="KPB88_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG0054643.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLVYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(12280..13206)
                     /locus_tag="KPB88_002375"
     CDS             complement(12280..13206)
                     /locus_tag="KPB88_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0054644.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNLYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTENSTYKNPESIHEII
                     IRKLNNESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4431 a         2224 c         1484 g         5176 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaaatatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt ttcatttatt tagatttttc atttgtttta gtttttattt
      841 tatttaaagt tttcacttat ttaagttttt attttcctta aatttttcat ttatttgtta
      901 agtttttctt ttccttaagt cttttattta ctttggcatt caccattcca aactatatct
      961 ctatatttat cagggtcagt atctccttct gtacttatta ataatacttt agagttctca
     1021 tctagtttca aagcttctct taagtctttt aaatcttcac tttcaaggat tgaaactaat
     1081 gctccagttg taactgctcc agattctcca gagattactt tctcatctcc tcttagaggg
     1141 tttcctaata ttctcatacc tttagcagat acccagtctg gtgctgaaac gaatgctgat
     1201 gaataatttt ttaacacttc aaaacctata gtgtttggtt ctccacaagc aagccctgcc
     1261 attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat
     1321 ttatagtaac aatcagcctc atcagcctct actactactg ttattgggca ttcatcatat
     1381 attgaagcaa catatccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat
     1441 acgtgtgttg gtctatctac tccatattct tttaattgtt ctatagcttc tttagccata
     1501 gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct
     1561 tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca
     1621 tcatagttta agtctgttat actagcatct gcaccttctc ctttgatttt atttaatcta
     1681 aattcagaag aaccttttgg cattaataca acagattttt gacctaattt attagctgtc
     1741 caagcaactc ctctaccatg atttccatct gtagctgtaa agaaagttat ttctcctaat
     1801 ttttctttta tttcttttga agttaattta tcatacccta actcagatat atctgtattt
     1861 aatctttgag ctaagtatct acccatagag taagaaccac ctaaaacttt aaatgcgttt
     1921 aagccaaatc tataagattc atctttaaca tatattccgc ttacgcctag taattttgct
     1981 aagttatcta atttaactaa aggagtcttt tcatattgtg gaaagctttc atgaaaagct
     2041 ttagcctttg caatctcttc atcttttaaa aattcaattc ctttttcttt ttcctctgat
     2101 ttaggtaagt tatttaattt ccattttatt tctctcattt attttatatt cccctttcaa
     2161 atttttcggt gtcattttaa ttttttatct ttgtgcctta tagcaaagca attaccgtgc
     2221 caatattatt tattctaaaa acaatttttt tatgttggaa ttacatactt ttataaaatt
     2281 tctttaaagt ttataaattt tgagtgtgaa aaataagatt tataaaagaa aaaattctca
     2341 tattgggaaa tttttatcaa tacgagaatt atcaattcga gaaatcgttt atttgtctta
     2401 attcttcctt tattatgctc ttatttttta tatgtctatt gttttgtcag atatattact
     2461 acttttattt tctataaata tatggctata accttcttac gcataaactc tataaattta
     2521 tatactttat attctttatt acccaaatat tctttattac ccaaagcctc caaaatggct
     2581 taaatctttt catagcttct tacagaagtt tacatgggcg tccccccaat aagcaaaagt
     2641 gcaatatgtg taccattgtg ccctacttgc gactattgag ctgtcgaaat tcgcaacttc
     2701 gcatttatct gatgaaatgc actatgcaaa atattcataa catccacaac agagttaaag
     2761 ttacctattc atattcactg taaaaatttc ttccacagga aatgtaattt taaccaatcc
     2821 tattgagaca aactcaccac ttattttttt gaactcacgc agtaatacat cacttgcccg
     2881 aaaaaaagcc tcactttcat catctccagt aagtgctatt gtgcaatggg gtacccaacg
     2941 attggggtgt accattccca accatttgaa tcaaaatctt gtagtacctc atataattct
     3001 ctctgaaact gatacatact gctattcatt ataggtgata caaacaccgt tttagtatca
     3061 ttaaacatgc ctactgaacc aatgtaagca ggtattacct tatggttttg tgcaaacccc
     3121 tttagcttgt ctatgcaaca agcctcatcc acatcattga aacaagccaa agtaagatga
     3181 ggtcttgttt tccattctaa gtacttcgta ctaatttttt catcagcgat ttttttagat
     3241 aaatcaaaaa gttgcttttc cgttttttta tcataatata gctctatcgc atattgcata
     3301 ataacacctt caccataaag taaaaatacc tatctataaa aacacacaaa tacctatcat
     3361 tttaatatta tattactact gttcctcttc caattttcta tatagtgtag ccaaacctat
     3421 gcctaagtct tttgctatct tcttcttcgt ctttgtatct cttccatact tattcaacaa
     3481 cttgtttatg taagtcaact ctaattcttt aattgaaagt atcctttctt gattctctac
     3541 tatccctcca actatatcat cttcaaatat tatatttaca tccttatttt tataaatatt
     3601 tccattaata ttataatagt ttaaaatatc taatggaagc atatctttat atattattcc
     3661 atcatctcca accaaattca tcatatattc aactgcattt tccagttccc ttatatttcc
     3721 cggccaatta tagttaatca acatattcat tacattttct tctattttat ttaactgaat
     3781 acctaattct aaagaatatt tagaagcaaa cttcatcatt attgcctcaa tatcatcctt
     3841 tctttcccta agaggtggca aatttatagg aattacattc aatctatagt ataaatcatc
     3901 cctaaactta ccttcattta ctaatttcaa taagttttta tttgtagcag caatcactct
     3961 tatatctaaa tctataagtt tatttgaccc aattctcgca aattttctct cttgtaaaac
     4021 tctaagtaac tttgcttgta aaggcattga caaatctcct atttcatcaa gaaatataac
     4081 accttcattt gcaagttcaa atttacctac tttccctcca ctactagctc cactaaatgc
     4141 accttttata tagccaaata gctcactttc aaggagattt tctggaatag cagcacagtt
     4201 tatcgcaata aatggcttgt tccatctgct accttcagca tgtatagccc tagcaattag
     4261 ctctttaccc gtaccacttt ccccagttat aagtacagtt gaatttgact ttgctaattt
     4321 cttagttctc tcttttactt ttaacattgc ttctgaattt ccaattatag actctatatc
     4381 actatttccc cagccactat tcacttttcc atgtccttta tgattgatat atgtcttgtc
     4441 aaatattatt attttgtcgt attgatttat atatggatat accggaatta ttttggcatt
     4501 tatattataa attttgttat caatatcaat ttctagtaat tcatgtccat ttgaggaaga
     4561 ttcaacagat actatattta ctatattttc tattatattt ttatctatat ctaatttttt
     4621 tagagctata ttgtttatat aagatatttt gctatttatg tctagtatta taacgccttt
     4681 ttcaacattt tcaatgattt ctatcatcat atccattttt ctactgctat tttttgacgc
     4741 ttcatgttct tctattttca tagaaattaa atcgcatata tgattgacaa attttaaata
     4801 attatctatt tttgcactta tttctacttt tttagtttta tctgtactaa tagcaccaat
     4861 tactcctata gttcgattat tatattttat tggaactgct attattactt tatttaggca
     4921 tttcatgtaa tgggaacatt ctatacaaag ctgattatca catatatcca atacgactaa
     4981 ttcctgacct gtctgtataa cattgtcata aataaatcct tctgtaacaa cgctttcatt
     5041 tatctttgat ttatacagac ctgtaccact aattcttatt aatctatcgt caactatttc
     5101 aatatctaca tctaacacac tagctactgt ttcagtgtat tttatcattt cttgttgaaa
     5161 ttcttttaat ttacttaaca tagtttcccc cattagaatt taatttacca aattatcagt
     5221 ttagctatat tttaacatat agtcactctt taaaatatat ataaaacatt acttatttat
     5281 atatttgcgt taaaacagac aatattaatt tataaaaagt ttgttttata tagtaacctt
     5341 aattataata tgcagacaat aaaataaatc acaatacaat aataaaaaaa agctttcctc
     5401 atataacaaa tatatttttg acataaaaat atacattata tgagaaaagc tcaatagtta
     5461 aatatttaga tagattttta tatagacttt tttaatataa acttttttaa ttgtagactt
     5521 tttttattag tttttaattt ttattagttt tgttattggt tttattgtta taatacttat
     5581 aatactgcca ttgcttcaat ttctaaaact gcatcttttg gtaattgagc aacttgtacg
     5641 catgaccttg ctggtggatt ttctttaaaa tatgtagcat atacttcatt aatatcatta
     5701 aaatccttta aatcttttac gaatactgta gtcttaaata ttttatccat acttgtacca
     5761 gcttgttcta atattgcttt tacattatct aaagaagctt ttgttgcttc ttttgggtca
     5821 gatactaact cgccagtctt catatctatt ggaagttgac ctgatgtaga aactatgttt
     5881 ccaacttgaa ttgcttgaga atatggtcca attgctcctg gagctttctc tgtagctatt
     5941 actacttttg acatgacttc acctcctatt attaagttat atttacagga aaataaaatt
     6001 tcctaaacac caatattaat acgaaccttt agattaaatt tcaaatctaa aacttcaaat
     6061 aatcaatact tagatgatta tgtttatttg tgtcttgtta acttttatta aagcaattaa
     6121 cgtgccaaac tttatttata tataaaattt tgtgaagtca ttcaaaatag ttacttctga
     6181 attttttcgt aaaaaaacgc tctaaaatta cttttttatg atgtcaattt ttttctcata
     6241 tctagaaatt taccaacatg agaattcttg ttttgagaat ttacagatta aagttatttt
     6301 ttaaccttaa attctgaacc tcccatgact aaagtcacag ggttcctgct tcatagaatt
     6361 ttgcataatc aagttattac atatgaaaga actatagttg acaatcccac tattataaat
     6421 aataaaacct ttacccatac cttatttacc ctagttatca tagagcctat tgaaaatata
     6481 acagctaaat ataataataa ccaaaaaatt atcaatatct accactgttg ctgaaaatcc
     6541 aaaaaactac cttttgatag tattacttaa caatctaatt aatctcataa taaatttaca
     6601 acttataaat aaattgttat attggccgta tctcaacaac tttctccttt cctagtttta
     6661 aatcttttat agaattgtac cacgtctaag taaattttca tactattaag tcttatttct
     6721 aataaatcat tagctctcat tctatgccca ataatgtaag attgcagtaa ttttattgat
     6781 tttaatagtt ttatttaata tacttatatt aattattttc atatgaaatt attatttata
     6841 tagggggatt tttttaatat gagcaaaagt gaattaacag cagaaacaac agaagaaatg
     6901 ttaaaagtac taagtggtaa agattatgat attgcatgtc atttacatga acttggtaaa
     6961 tcattagatt gtaaaattga accaaaaaca ggtgctcgtt cttacaaaat agtatattca
     7021 actaagaaac caaaacgtag cttatttact attgaatgta atgaaaaaaa atggagagtt
     7081 aaagcaaatc tttttcatct aaatacatat aaagatgctg tggaggaatg ctctaaaact
     7141 attaaagata gtataactaa aactcgtact tgtaagaaat gtaattcaaa gtgtattgga
     7201 ggttcttcgt ttgaattaga tggaaagtct tacctgactt gcataggaag tggtcattat
     7261 tttgcaaata tggaagaaat ggattggaaa aacctagaaa aattaattac taaagaaaat
     7321 aatattatgc aggaatctgt atagtttttt aagggtaatt attaaaatat atgagcaaca
     7381 acagatactt aaataggcat aaaaaaataa agggtataat aaggatatag aataaaaata
     7441 aatgatatag cacaaagagg ctgtctcaaa atacagataa gcctcttttt attgttgaaa
     7501 tgataagaat atattcaatt ttatacaatt ttatacaaac atactacgcc aacttaccca
     7561 tttcatatgc ctgtttcatt gccttacttc ccttaatatc acctacattc caagcaccta
     7621 caccatatac tatacctttt tccttagagc catttagaca tgatgtaaat cctctaaatc
     7681 cttctattgt tctttccata tcagccacat tttcatctgc tgctgtaacg ataaaataga
     7741 aatctttatc acaaatttca gtatatctag agcaagttct atcaatcata gttttcattt
     7801 gcgcattcat agtataaaaa taaactggag tcgctaaaac aatcacatct gccccaatca
     7861 ttttttcaag aatttctccc atgtcatctg tctgtgaaca atttgcttct ttttcataac
     7921 aagcaccaca tccagtacaa tagttaatat ctttatcttt taaaaatatc ttctccactt
     7981 gatttttaga ttctaatacc ccttgtataa actggtcaca caataaatca gaatttccac
     8041 cttttctagg actagctgat actattaata catttttact cataagatac tgcctccatt
     8101 ttcttatttc tactgctctt taatactttt tctttctcaa taataccttc ttcatatcta
     8161 tcaatcttaa agtttaatcg ttccaaagtt ttttgcattt cttctattct ttttgctagt
     8221 tggtttctct gctctattaa aagttctttt ctagctttga tagtactgct cccttgttga
     8281 aacatagcta catactcaat taatgtctca atagaaagac ctgcacctct catacacttt
     8341 atgaaattaa cccactcaca atcttcttct gtaaaagacc taattccact tttatttcta
     8401 tttacaggtg gtattaaacc aattcgttca taatatcgta aagtatccgc tgaaagctcg
     8461 tattttttac taacttctga aatcgtcata catatcttct cctcttgata ttgtatagat
     8521 tgaattattt ctcaaaggtt tctgctacat tctttaaagc atctataatt ttaatgtgtt
     8581 gaggacagct ttcttcacat tgtttacatt caatacaatc tgaggccttt ccataagttt
     8641 tagtataatt attatagtag accatctgtg ttgaaaatcc agtattaaga gcttgttttt
     8701 ctgcattgta caacgcaaaa tagtttggaa tagcgatatt ttttggacaa ccttctacac
     8761 aatactgaca ggcagtacaa ggaactaaaa ttgattcgtt gataatctct actgcttcat
     8821 ctataatatt atattcctct tgtacgaatg gtttgaaatt ttgcatatat cctgtattgt
     8881 ctaataattg ttctatattt gacatcccac ttaagaccat cataacatta tctttacttg
     8941 ctgcaaaacg tattgcccaa gatgaaattg acatatctgg attgtagtca ttcaataatt
     9001 tctctgcttt ttctggtatc tttgcaagtg tacctccttt tactggctcc ataacaatta
     9061 caggcttatt atgcttctct gccacctcgt agcattttct agattgtatg ctttcattat
     9121 cccaatctaa ataattaatc tgtaattgca cgaagtctac ttctggatgc tcactcaaga
     9181 ctttatccaa taattctgca ctatcatgaa aagaaaaacc aatattcttt atttttcctt
     9241 cttttttctt ttcttcaata aacttaaagc tatcaaattt tttagctact tcataatgtg
     9301 acacgcctat attgtgtaaa agatagtagt caaaatagtc tacaccacat ttttctaatt
     9361 gctcattaaa aatggtttct tgctcttctt ttttctttaa agccattaat ggaagttttg
     9421 tagctatagt aaaactctct cttttgtgtc tttttactag agattctcta agtgctatct
     9481 cactttttcc catgtgatac acataggctg tatcaaaata tgtaaatccc ctttctaaaa
     9541 aagtgtctac cattttattt aaatgttcca tatcaatagt tgtaacatca ttttcatctt
     9601 ttattggtaa acgcattaat ccaaatccta atttttttgc ctccattgtt ttacttcccc
     9661 ctttaaaact tgtttgtttt ttctctctac tttaactatt cttacaagta tataattctt
     9721 gtataattat actttactac ttggagttaa cttcaagtca atagattttt ttatttttaa
     9781 gacaaatatt ataaaaatac agttctttaa tattaatcca ttacatgttt ctttcaccct
     9841 ccaattctca aacttcacaa actccaacat gtaaagaaat atatacttta agaaattcag
     9901 tccattaata ttactacaaa tactttttag atatgtttaa aaagaaattt cacctaaaat
     9961 atttataaaa gatttagaaa taggtcttgt gtctatttta aaatatataa aacagtggta
    10021 aagacttatt tataagataa aaaatgaata gtgtataccc tttttaaaaa gttttttcct
    10081 ttttatattt gtgatacaat gttaaatata aaaaggaaaa ttttgtgtta aagaaatctt
    10141 atttgttgat tttgtaactt caaactcagt atcaatttga ttttaagatt atattttatt
    10201 aagtatgcta aataacaaat ttttaaagat tgtgaggtaa aaatatgaat aagaaaaatt
    10261 tagctatatt aatatcaggt atgatgtttt tttctagttc ttcagcaatt tttgcagaca
    10321 atactacaaa gcaagaaaga ttaataggta aaacaagata tgaaacagca gtagaagtaa
    10381 gtaaattggg atgggttcaa tcaaaaacag ccattatagt taatggtaat tctattcaaa
    10441 gtgcgctttg tgcaaatcca tttgctaagc taaagaatgc acctatactt ttagtaaaca
    10501 acaacagtat tgaaaattca actaaggctg agcttaaaag gcttggtgta gataatgtgt
    10561 acattgtaga tagtggaaat agcatttctt ctaaagtaga aaatgaaata aaatcactta
    10621 acataaaaat aaataaaata gtaggaaaca atatatatga aatgtctaca aatgtactta
    10681 aagaaataga taagataaaa aaggtagaaa atgttgcagt tgtaaatgga ctaaagggac
    10741 tatctgatgc agttagtata gcttctccag cagcaataca taatatgcct ataatattag
    10801 tatcagatga aaacagcaat gatagtagta taaatttctt aaacaatagg tctataaaaa
    10861 aatcttacat agttggagga aataagattt tatcagacaa tacagtaaag aaatatccaa
    10921 acagagaaag aatcagtggt tcagatagaa acaatactaa tgcaaaaatt attgataaat
    10981 tttataaaga taaaaaaata aataatgtat ttgtagctaa agatggtatt aaccaagaaa
    11041 atgcattagt agattcatta tcaataggag tactagctgc taaagaaaat tctcctataa
    11101 tgatatctaa tggtagcctt gtaaatagtc aaaaagaatt tattaaaaat aaagatatag
    11161 atgaaataac acaagtagga ggaggatata atcaaaaagc atttgaagat attttaaaac
    11221 taaaaaatgt gaacataaga aataaaacta aaaaaatatc tttaatacag gctaaagaaa
    11281 tattaaaaca agctcttcca ggaagcaata ttttagtttg ttattatgat gaagatgata
    11341 atgaatatga ctgcaaagtt ataaaggata atatcctata tgagatagaa attgatgcct
    11401 actctggtaa aataatagaa attgaaaaag atgatgactt aaatgataat aatttaggta
    11461 cacaaaatat atcattggat aaagctaagt ctatagcatt agatgctgtt cctaatggta
    11521 atttagtcta ttgtaaatat gatgctgaag ataatgaata cgaggctaaa gtaataaaaa
    11581 ataagaaaac atatgaatta gagataagtg catttgatgg aagaattgta gatattgaca
    11641 tggacaatga ttctgatgat gactttgatg ataatgataa gaataactct aatactaatg
    11701 acaacagttc tattattagt gctgaaaaag ctaaatcaat aatgctagat aaagtgcctg
    11761 gtgggaaaat agtaaaattt aagtatgatg aagatgacaa agaatatgaa ggtgaaataa
    11821 taaaaggaaa aactgaatat gagataacaa ttaatgcaat tactggtaaa atattagaat
    11881 ttgaaataga tgattaataa aaaatataaa tagatgatta ataaaaaata tatattctat
    11941 taattattac aagcaataaa gccataaata ttgaaatttt caatatttat ggctttattt
    12001 tattttttta atttaaatcc acattatgcc attatatata cctatgcact cttgtaaaat
    12061 gggttatatt tctccagtat attaaaaaaa gtgagtgtct caaaataaat cttctaactc
    12121 ataaagcact ctcctttgta ttaaatcaaa tatattttca tcatttaaac aattaagaag
    12181 aggcttctct ctattttgag acaactccat tttaatacac tatttataca taaacatatt
    12241 ttaaacaact aaaaagcaac taatatctta ataaatatct caatacatat cttcataaca
    12301 catatcacca taattacaag cagataaaga actctcctca tttaaatacg actcaaattc
    12361 atacccaaac agaattgttg gttctatata tatttccata tctgtatatc tccatcttct
    12421 tacctttgcc tctataacct tgtaaaaatc ttctaaacta tacccttctt ttaatctttt
    12481 atctataagt gccttagtta tactcaaatc agctttaaaa ttttttcctg attcattatt
    12541 taattttctt ataataattt catgtatgct ttctggattt ttataagtag aattttcagt
    12601 caaatcttca tccgaatttt caaaggtatt tttatttaaa ttctttttat ttaaattatt
    12661 ttttaataat tcttttttct ttgtctcatt ttctgtttca tacccagtat gaattatcaa
    12721 atcataatct ttgtctatac tctcttgatt tatacggctg tataggttgg tattactgtt
    12781 cttaaacaac tcatctctat gtttatattt cattgttttt tggttattgt taatagttgc
    12841 atcaatgtat ttctctatat ttttgaaaat atcaccattc tttgttacaa tttctgcatt
    12901 actactttca gaacaaacat aactatatac agaacaacaa tttcccttta ctcccctact
    12961 tattaatctc aatataccta aatctataaa ttcatttaca agtctactta ttgtagatat
    13021 agatacatca aggtcagatg cagctttcct tactgtcatc ataaattgtc ctcttggcag
    13081 agttcctttt aatctttgta tattttcttt tctcattaca tagtgattaa accttattct
    13141 atttacatct cttttttcat taaaatataa tactgctgaa tttaattgat aaaaactatt
    13201 actcatattc ccactcctcc aatctaaata gataatttgt tgtaaagttt ttagattttt
    13261 aaataactta ttaaatgatt atttttatta aaaagtattc tttgatattt tcatt
//
LOCUS       DAELJO010000101        13267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELJO010000101 DAELJO010000000
VERSION     DAELJO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.101"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(155..1036)
                     /locus_tag="KPB88_002376"
     CDS             complement(155..1036)
                     /locus_tag="KPB88_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG0054645.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1492..2466
                     /locus_tag="KPB88_002377"
     CDS             1492..2466
                     /locus_tag="KPB88_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054646.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2582..3643)
                     /locus_tag="KPB88_002378"
     CDS             complement(2582..3643)
                     /locus_tag="KPB88_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0054647.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4252..5103)
                     /locus_tag="KPB88_002379"
     CDS             complement(4252..5103)
                     /locus_tag="KPB88_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0054648.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5120..6040)
                     /locus_tag="KPB88_002380"
     CDS             complement(5120..6040)
                     /locus_tag="KPB88_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG0054649.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6150..7064)
                     /gene="murQ"
                     /locus_tag="KPB88_002381"
     CDS             complement(6150..7064)
                     /gene="murQ"
                     /locus_tag="KPB88_002381"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG0054650.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7144..8241)
                     /locus_tag="KPB88_002382"
     CDS             complement(7144..8241)
                     /locus_tag="KPB88_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0054651.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8474..9820)
                     /locus_tag="KPB88_002383"
     CDS             complement(8474..9820)
                     /locus_tag="KPB88_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054652.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9909..10217)
                     /locus_tag="KPB88_002384"
     CDS             complement(9909..10217)
                     /locus_tag="KPB88_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054653.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10479..10877)
                     /locus_tag="KPB88_002385"
     CDS             complement(10479..10877)
                     /locus_tag="KPB88_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054654.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(10879..12039)
                     /locus_tag="KPB88_002386"
     CDS             complement(10879..12039)
                     /locus_tag="KPB88_002386"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG0054655.1"
                     /translation="MITMYSVGLMSGTSLDGIDAVLAEISGNGRNTKVKQIEFITLEI
                     SKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12470..>13267)
                     /locus_tag="KPB88_002387"
     CDS             complement(12470..>13267)
                     /locus_tag="KPB88_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0054656.1"
                     /translation="NSGEIDSYLVDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKE
                     LDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTM
                     FEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIIN
                     NAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYK
                     STGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         4233 a         2345 c         1470 g         5219 t
ORIGIN      
        1 ctagtaataa aaaattactt tcccaaagta gatataagct tcattttatc gttcaaataa
       61 tgaaaatata tttgatttca tgcaaaaaga gtgcctaatg aactaaaaag ttcattttga
      121 gacactcatt tttttatata agtttatata gtttttaatc gttcgcattc aaccactcag
      181 ttaagacttt tatatatttc tcattttctt caacgaatgg catatgtctt gaatactcaa
      241 ataattccca cttactatta ggtattctat catacatagt ctttgcaata tatggtgaac
      301 ataaatctat tgctccacta gttactagac aaggctcttt tatttcatgt aatctatctg
      361 taaattcata tccagaaaga gttcctgtag gagaaaattc attttgtccc catcctacaa
      421 tatatgcttc agaacctgat tttttaggtc ttctcaggca ctctggagaa tcttctgtca
      481 cttcacctgc acaatacatt tccataaatc tttctaatgc atcattatat tctttgctag
      541 agtaatctcc agtatttact gcatcaagta atgctttttg gtcagcctca ctcatatatg
      601 atattcttct tttttgttct ttctcccaaa gttttgcaga agaaagagta gatgaaagga
      661 tgtaagactt aatacctttt ggtttatatt caatagcata ccaaatagcc tgcatacctc
      721 cccaagattg acctaacaga tgaatttcat caagtcctag atgttttctt agttcaataa
      781 gctcctctat ccaagtatct gcattaaata gctcaggatg cccttctaca aatgaatttc
      841 cacaaccaat ttggtcatac attattactt gtctaccact ttcagctatc ttatccaata
      901 cctcaaaata gttgtgtgta gaacctggac ctccatgaag taatactaat ggcttcttcc
      961 cttccgtatt ttctcctact atacggtaat acgttttaaa acctttaaaa ggcatatatc
     1021 cttcagtaat tttcatgttt atctcctcca atttgtaatt ataaacaata atcaagttca
     1081 ttaacaatac tatttaaaac ctcttataca tatttgatta tgtattttaa tctttatacc
     1141 tacttttata aatagtataa cataaaagtt aaaaatctct atactaaacc aattataaaa
     1201 tcatattaac tatataattt tattttttaa taatagtata tacatatttg attattttca
     1261 tttgctgctt aaaatcaatt ttaagttttc ttcctataat aaacttcctc tttgtggtct
     1321 catgttagtc tctaaattta aaattttaca aaaaattttc ataaaataaa attatattga
     1381 ttgtatctaa acaaataggg caataaaata attaagtata gttacttacc tttcaagcaa
     1441 tattagtttt atgttagaat tttattatat tgatttacag gaggtttgca tttgaataat
     1501 attaattcac ttaaagtaat aaaattatct cttggatgtt ctcttgctat atttattgct
     1561 tggctattaa aattagaata ttcaatggtt gctggagtta tagtactact tacagtaaag
     1621 gacactaaaa gagaaactct taaaggctcc attggaaaga tttatggttt tcttttatgt
     1681 actatttttt catatttatg ttttaatatt ttaggatata atttgacttc attttcaata
     1741 tttatattta taattatacc actttgtttt ttgttaaata ttcaagatgt tattgctatg
     1801 tgtgtagtca tagcatccca ttactacctt caaggagaaa cttctatcaa atggatttta
     1861 aatgaagctg gaatatttgc agtaggaact ggaataggtg tattaataaa tatgtatatt
     1921 ccaacaaata tacataaaat ccatgaagtt caaaaaaagt tacaggaaga agtgagtatt
     1981 gtacttatag acattgcaga tattattgta aatcctaaaa aagaaaatgg atatagtaga
     2041 gatttatata cattaaattc acttattgac agcagtattt ctgaaactta cgataatatt
     2101 aataatactt tattaagtga tactagattc ttcttagagc atatggatat tataaaaagt
     2161 caacgggata tcttagaaaa tttgtatagt tatgtatctc aactaaattc tacaccacca
     2221 caagctcata ttctatctgc ttttatacat aagataggtt atactgaatt tgaagctgaa
     2281 acaggcaatc ttcttcttga agaattaaag agactaatgc ttagtatgaa aaaccagcct
     2341 ctacctgttg acagaacaga gtttgaaaat agagctattt tattcctatg cttgactgag
     2401 ttaaaacagt ttttagttaa tcgtaaacat gctcagatgc ttagagataa taatttttat
     2461 aaataaaaag gggagaaatc tagcagttga cctgactaca aaatatcaga tataatatta
     2521 aatgcgcccc tagtaagagg gcgcaatgtt tataacttat tctactgtca tgattaaatc
     2581 attattcata tcgaacatac attatttgac caagtccaat attagctttt tcatcagtga
     2641 tttctatttt aatagtcaca tcattttttt tacagtactt cgctaattct gcaccagaac
     2701 gatttttacc actaaaatct aaagaaaatg gcttatcata tttaaaagga tattcaacag
     2761 gtttaccctc tggtgctaat gcaaacatcc catatggtgt gttcataaat cctattacag
     2821 caatcgtaag atttgatggg tctaaatttg attcactacc tgtaaatgta attgtattta
     2881 ctgatgaatt aactacacta catcgaacac cttcatacat tacatgtttt tcatttatat
     2941 cttctccttt ttttccaaaa tttccaacag cctttgcccc ttcttgatgt ggtaacatag
     3001 tgtgcttccc aggtggtgct ggtttctgtg tctgttctgg ttttactgtc tgtactattg
     3061 caccaacttt tggatatgct gtcttatcaa atggtaatgg gtggctaaga ctcttgtata
     3121 tgatatcctt tcctgctgat ttattagcat caatatctac atgatacaaa cgcccatcac
     3181 caaaacggat aagattccac tggtggtcat ttgatgtgct gtgtacataa taagattcaa
     3241 ttcccatagc atcacacaaa gctttcatgc tcctagccat tcctgagcat gcaccttttt
     3301 tagtaaccaa tgtataggca gtatttgctc cctctactgc attccagtca acatatgttg
     3361 tgttattttt aatgtgattg taggcaacta aaatcttagc atctgtggtc atattctttg
     3421 tgattttctt gttcataaaa tctgctgttg cttgtgctac tgagtttaat tgggcttgtg
     3481 acagctttgg accatatacg ctattggttc cagtgacata gctttttggt gtttttgctg
     3541 ttacctcttt ggctgtcata ccataaactg tcatactcat acctgagatt aaaactattg
     3601 ttgtaatcaa taacttggta gctaacttcc taattttctt catacttaat attctccttt
     3661 ataaaacatt gttacttcct attctctacc attaataaaa ttaatttagg atttaaaaag
     3721 gtggattgtc aacatgtata tttctccatt catacattcg tgaaatattt aaatttcatg
     3781 gaaaatctaa tagatatttt ctagataatt tgtatacaat acaactcgca aatcagggtg
     3841 tttgctgtta gtttgagaaa atgatgatga ttaatacatt cggaatcttt gacctataat
     3901 aaataccatt gtcttcaatc attaataatg gtaagagtaa agagaattat caataataca
     3961 gttaaaatca actttgtata catttcatac attctctccc aacaacatta tacttcttat
     4021 aatttgtata ttcaatacgt tttctgggaa aatataggat atatctataa aaatataaaa
     4081 aaggagttga acttaaaatt tgattaaaaa aaatcaattt taagacaact catttgtatt
     4141 ataaaaatgc tatatattat taagtaagtc aatattttag atatttatct atttcttatt
     4201 gacttacgct tatagcctaa cttatttgtt tagttgatat ataacctagt tttaagcatt
     4261 attaatattt aaataatcaa gtatcctttt tgtttcttgt agtttctgtt ctgcctcatt
     4321 taagttttct tgaattatag aaatatatat tatatctatc aacgtaagca ttgctattct
     4381 tgaagaaata gctccttctc taagtctttt ttctatattt ggtactacta gctttaaatc
     4441 actaatctta ctaagatgat tgtcactata tttagttata caaatgacct tacatccttt
     4501 taattttgca ttttctatag actttattac ttcttttgat tctccagagt aagatattgc
     4561 tatagctata tcttctttat ctacatttga agataccata agttgtgtat gactatctaa
     4621 agaggtaaat gcctgtttat ttatccttaa aagtttcatc tgtaaatcta gtgcaactaa
     4681 agccgaacca cctactccaa atatatatac atttttagcc tttattatct gttcaactgc
     4741 ttcttttatt atatcttttt cattcaacaa ccttgtttgg ttgatagtct ctatgttttt
     4801 gtttgtaatt ttgtttataa cactatctat attacaatca tagttttcat ctacatatgt
     4861 gtattcattt tctttgccca tcaaatcttt agctatctca atttttaaat ccccaaaacc
     4921 cgaatatccc atttttctag aaaacctaac tatacttgct gtacttgttt ttgagtttga
     4981 cgccaattct gatacagaaa gcttacttat agcctctgta ttattaagta tgtaatcagc
     5041 tattttttta tctacttccg ttaaatcttc atatatctgt tttaatttta aaatgcaact
     5101 catactctat cacctctacc tatttactat aatatttctt cttagcaagt aaacatgctc
     5161 ctaaatctgg agtcaacact ggtctcacta taccaaatct cgttttattc aaatattttt
     5221 taagtggctc taatattaaa tctcctgact taaacactcc acctgaataa gataccttta
     5281 tctttgtacc ttgttcaaaa tgatgctcta atcctattat aagcctactt aattcataag
     5341 ctgcatcatc aaatatttta ctagcagtat tatcaccttc aataactgct tttaaacata
     5401 tcgtagcaaa ctttgctatt tctattctat ctccttgtat ttcatcattt acatatttta
     5461 ttatgtcata atcattctct atattaagat gtctttttat taaatcatat aattttgttt
     5521 ttgaacacct tccatcagac tgcattgtga acatttttaa ggtagccatt cctatataat
     5581 acgctgaacc ttcatctcct aatacatagc cccatcctcc acatctgtga agattcccat
     5641 ctttatctaa agcctgtgct attgaaccag tacctgcaat tatatttata cctttttccc
     5701 catttaaaga acctgctaat gctatttcta catcattacc tagagtataa tttatatgac
     5761 tatatgcatt ttttgttgct acttctaatg catacaatac ttcttttatt tttccatatc
     5821 cagcaagtcc aacaaatgca tacgttattt cttctacatt tatcttagca tctttacaaa
     5881 cttcttccaa cccagttatt aataatttcg ttagattatc aaatcctatt tgattataat
     5941 gacaagttcc tttggttatg gttcctacta tatttaattc ttcatctact aatgtaaatg
     6001 ttgttttcgt tcctccacca tctacaccta aaaaatacat actatcctcc tacaatatct
     6061 ttaataattc tttcaatact taatatctaa aaataacctt ccaatgctgt ttttttaata
     6121 ttgaaaggtt atcagtacta ttatttattt tacaattttt ctattgattt taaagcttca
     6181 cttatataac cttcttgtct atctaggata ctctttgcac tatccttatc taaaccactt
     6241 tttatcataa atatagccaa tttaacgcta ttatctgctt cttctaagaa tttttctgca
     6301 acatcatatt ttacatttgt agctatcatt attattcttt ttgctctttc aaccaacttt
     6361 tcatttgatg tcttaacatc taccatcaag tttgagtata cttttcccat cttaaccata
     6421 gaagctgttg ttaacatatt acataccatt ttttgagctg taccactttt cattctagtt
     6481 gaacccatta ttacttctgc ccctactata atgtttattg gtaaatccgc ttttttactc
     6541 atttcactat tcggattcat agttattgat agtactgcac accccagttc cttagcatac
     6601 tccattgcac ctataacata aggagtcctt cctgatgcag ctataccaca tataacatcc
     6661 ttagatgtaa ctcctctatc tattatatct tgctttccaa gttctttatc atcttcagca
     6721 ccttctattg ctttaaatat tgcttgattt ccaccagcta ttatagcttg aacaagctca
     6781 aaatccactc cgtaagttgg aggacattct gtggcatcta gtattcctat cctcccactg
     6841 gtacctgctc ctatatagaa taatctacct ccctgcttca ttctatctac tattatatta
     6901 acagcttttg ctatactctc cttttgttct tcaacagcat aggctacagt tttatcttcg
     6961 ttatttatta attctattat gcctaatgta tcttctttat ctatttgcaa tgtattctta
     7021 tttctacctt ctgtaactaa attttctaaa gtttttatta acatatcatt tcctcatttt
     7081 cttaatatta aatttttaaa cacattttaa ttccaacttt cataattata tttttataat
     7141 aaattatttt gttgagggtt taaaattaaa cttttgccat ggtttaatat aatctaatat
     7201 aaataattct tcttttctta tatatcctac tacattcgat ttaccactgt ttttcatatc
     7261 agtcaaggct atttgtaact cccctgcata atgaccatat tcactacttt caattactat
     7321 atctcctctt tttattattt taggagtatt aaatagttca aatttgtatc ctttatattt
     7381 aacacgagat tgagtagacc ttatcagact gtcagatatg tcccctctat taaaatgtat
     7441 ttcttcaaag agtatcttct tctcaacttc tcgtatattt tcctctattg ctacatcaaa
     7501 tgtaaccata tcttttctca tagaacctaa tacctttagc tcctcatatg ttggataaca
     7561 atttgatatt attatatcat ttatattatt catagctatt atatgcttta cttgaatatc
     7621 tataggtaga tttctgtgca tctcaattgt tggaagccca tcagttacag gccatggtcc
     7681 aaatgtggtt tcattttgac ttgttacaaa aactgctgtt cttaatccat acttattaaa
     7741 tctttctgta cattttataa agtgttctaa atttaatcca ctatatctat gtggataaaa
     7801 gttatgacat cctgtcaaat tttctttatt tggttggtag tccattattg tatctatata
     7861 attagtatta ttactcatat tgatttctat tttaagattt tgaggattaa aagtcattaa
     7921 ggcctcttca gaacctgtaa atcccatatc aagccttatt ccgtctgcct tcgtcttttt
     7981 aaaaaaattc aaatctttgt agcttatttc taattcacca aatactctgg gatttacatc
     8041 aagtattacc tcaaaacctt taaacttagc gtattcattt atacttttaa attttttaat
     8101 tatattttct ttagtatctt taactgataa taaacatgaa aatatacgtg taaatccatt
     8161 ttcagcagct ttatctatat aatcatatat ttccttagat gttgtttttt ctggataaat
     8221 tgatattcct aatcttccca tctgtgtcat ctccttatat cctatatgtg tttaaattta
     8281 tttcgaatta cacgaaactt ttttaaaaat ttgatgaaaa aaagtttcat atatatttat
     8341 aaaaaaagaa aagtttacct tttctttttt tataaacaat caacaaaata tcttcgttac
     8401 ttatttcata actgttatac tttgaaattt atactaatct aatatagttt ttaatttcca
     8461 atatagtttc taattaataa gcttcttgct cttcaacttt ttcttcatga agcaacctct
     8521 tatcgtacat cttgacaaat ggatagtaaa ttattattga tatagctata cataataagt
     8581 taagaactga agctctccaa tctccaccag tggctaaata tgcccctact ggtcctggta
     8641 atgtccaagg agcagtaact actactctgg atactaaatt agcactcata gcaaaccatg
     8701 aaactgttgc cattactatt ggtgctacaa caaatggtat tattaatatt gggtttaata
     8761 ctattggcgc tccaaatatt attggttcat ttatattgaa tattgctggt attacagttg
     8821 cttttcctaa agactttcca taagctgatt ttgctctaaa tgcaagaagt aaagctaagc
     8881 ctattgtagc tccagaacct ccaatccata taaaccattg aaaaaagggt tcagcagcaa
     8941 tacctggaac tgcttgaccc gcagcatatg aagctgtatt agcatctaat aattgtatcc
     9001 ataaaggtct tgccaatgaa cctattattg atacaccatg tattccaaat gaccaaaaaa
     9061 acgttattaa aaatattagt ataattactg atggtaatgt gtcagtggct gaaactaatg
     9121 gagttactaa tttagctatg aagccatgcc aatcaaaccc taggaaataa gtaacaatag
     9181 ccattaaaat tattattaat gcggctggtg tcagagcttc aaaagacctt gtgactgatg
     9241 ctggaactgc ttctggcatg gttatcttga atttagattt gtcagtaaat ctataaatct
     9301 caacacctat tatagatgtt acaatagcaa caaacattga acctccacca agattagcca
     9361 ttggcatttg ccatccttga ggaattgaat tataccaagc taaaagttct ggactctttt
     9421 caactatctt taataaatca ggggtaagac cctctattgc agtaggaact attgtcaaaa
     9481 ggaatgtagc tgtagctaat aaacctcctg tcaatccatc taacttatat gactttgcca
     9541 aactataacc tatgccaaat gtagaatata ctgatataat aaaaaatgtc attctatatg
     9601 gtagtaatat tgtaactgca ttttccttta gatactgagt taccccccat gaatctggta
     9661 atggaggaaa tgctaatatc aagaaaaatg acccaactat tataagtggt aatgaagcta
     9721 cgattccatc tcttacagct cttaagtgac gttgttctgc caatctggcc atagaagttg
     9781 ataatttagt gtctaaaaat tctgtaaatt tatctagcat cttttctctc cttaattaat
     9841 atttattttt attatcatta aaatttttat aaaatcaact ttcttctaac ctaacaacac
     9901 tgcaactact atttactcaa cagctcttga acttgtttta ataactttgg tcctcctaat
     9961 ggactataag cttgtgcttg tattaatgca catggtacac taacctcatc agcaaaagct
    10021 tttaaatagt caaatctatg tctgacttgt ggagctacca tagcaatatc ccatccattt
    10081 ttaacttcat cctcaaactc ttgagtacca acagccaaaa catctatatc tattccattt
    10141 tttgctcctt ctttttgtaa tgcattaact gctatagcac ttgacatccc tgatgaacaa
    10201 acaaataata ctctcatatt gattccccct gtttttttat ttcttttcaa tcattatagc
    10261 atgtattttt gattacagga atacgtttgt atatattttt tcatatttta catttttatt
    10321 tgtaatttta tttcatgaat ttatatcttg ctttaattgc tttatttatc taatcattct
    10381 tttgtctaaa attacacact acttaaaata ctcatatctt ttcttaaaaa agaaaaaaga
    10441 ctatcttatt attaacataa aatagtctca atactttttt aaatatgtat tcctttatat
    10501 atccagaagt aacctattat tgcaaatgat aaagcaaata aaatatacag tctaaaaaat
    10561 cccaaagaaa aacctctctt agtttcaaca aagtatcttg tatatgcaac tgcaaaagat
    10621 gtaattaatg aatttcctat aacaactcca aatctattat ctattccaaa tatagataat
    10681 aacaaaggaa aaactacagt acttacaaga gatgaaacta ttagtattaa tccactttta
    10741 aaacttatag ttggaatatt ataatctgtt ctttgatttt tctccatttg tttcttagta
    10801 tttttattac tttttgtact ttttgcattt ttataattat tatttttctt tcttttcttt
    10861 tttttattat tagacatgtc atttacctcc aaaaggttta ggtgttatat tccctaaaat
    10921 aactttattt ttagcccctg ttgcagatat tacattagaa aaagaattat ttaatgtttc
    10981 attgcccaag attacaaatg ccaaagcctc ctttgcatca gaagaatacc catattcatc
    11041 ttgagttaat acttcaacct ctaataataa ctcttttata tatcctatta aagttagatt
    11101 atgagctcct ccacctccaa ttaataaagt atctacatta acctttggaa gtatgaagtt
    11161 tttataatta taatatatag cttttgccgt aaacatagtt actgttgcta tcaaatcata
    11221 tttatctaca tgtttatatt tgttaagcat gttatccaaa tagacttgtc caaatacctc
    11281 tcttccagtg cttttaggtg gagcttgact aagatactta tgactcatca aatcttttaa
    11341 catttcttca ttaattttac cttttgatgc aaagtatcca tttttatcat actttctatt
    11401 aaataatctt tgacatactc catctattat catatttcct ggtcctgtat caaatgcaaa
    11461 tacatcatct atgttacatg atttaggtat tatagtaata tttcctattc caccaatatt
    11521 ttgaagtgct aaatttttat ttttatcact atataataaa aattcactat atggtactaa
    11581 tggagcacct tctccacctg ctgctatatc cattactcta aaattagata ttacctttgt
    11641 atttgtttca tatgctataa ctgctggttc acctatttga agcgttgaag atacaaaatt
    11701 attataactt cttggtatat gaaagattgt ttgaccatga gatgcgataa aatccaaatt
    11761 tgctatatga aagtttgctt tatgacacac tgattttaca gctttactaa ataaatatcc
    11821 aagcttaaaa tttaagctac agattaaatc aacactcgat tcctcttcta tacagcactt
    11881 tcttatttcg tcttttatat ctttagaaat ttctagagtt ataaattcta tttgcttaac
    11941 tttagtattt ctaccattcc cactaatttc agctagtact gcatcaattc catctaagga
    12001 agttcctgac attaaaccta cactatacat agttatcatc cttttaagtt tatagttatt
    12061 cctccccaaa tcaaggtatt aagtttaata attttagaat taatcttatt tcatctttaa
    12121 ttacgataat ttactcttat gaaattaatt gagttaatct tatacacgtt ttttacctta
    12181 gctaaatctt acattaattt attttcagtg tcaatgtaat accatgtaat tttttttcac
    12241 aaacaaaaca tattctctct ccaaaaacca tattaaaata gttactacaa ataaaaaaca
    12301 taccaggtac ctatctgata tgtttttcat atataataat tattatttta taatatagat
    12361 agaacatcat ttaaggcact aattaaatct tcactatttt caagaactaa atttattctt
    12421 attgtatatg tcgatttgta tatgatgata gattttatag tctaataacc taactatcta
    12481 ggcttaaata atccgatata tcataaaagt agatattaaa aattgtatat gaaccatttt
    12541 ccgaacatac ctcaatctca tgccctaaac tatctatcat tttctttaca aaatacatcc
    12601 ccattccagt agacttatac aattgatttc ttccattttt ccccgtaaat cccttgtcaa
    12661 aaaccctaga tatatcttcc tcatttatac ctataccaaa atcttttata gataatacca
    12721 ctccattttc taactttttt gaattaaatt caatcttacc aacttccttt gaatacttta
    12781 tagcattatt aataatttgg tctaaaacat atgacatgca tttagaatct gtgtaaacat
    12841 cattttctaa ttcattcaaa ctaacttcta cattattttt tataagcaaa aatgaattat
    12901 tttttataga catttttacc aatttttcta aattaaattt atttataaat acatcttcaa
    12961 acatagtgtt gcttctacta ctatacaaaa tagagtttac taaaaaattt atctttgcaa
    13021 cctgattctt aatatccaaa ctatcttcta tgctacttaa cctatctgta attatactta
    13081 aagaagatat tggaattttt atttcatgaa cccatttagc tatgtactca tccagttctt
    13141 ttaatgattg tttataagtt tctatgtcac tattattttt tatttttaag ttttctataa
    13201 tatcttttat tagattttct tcaaaagatt gtccatcaac taaataactg tctatttcac
    13261 cactatt
//
LOCUS       DAELJO010000102        13245 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELJO010000102 DAELJO010000000
VERSION     DAELJO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.102"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            271..1689
                     /locus_tag="KPB88_002388"
     CDS             271..1689
                     /locus_tag="KPB88_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG0054657.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1765..2577
                     /locus_tag="KPB88_002389"
     CDS             1765..2577
                     /locus_tag="KPB88_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0054658.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLDEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            3000..3617
                     /locus_tag="KPB88_002390"
     CDS             3000..3617
                     /locus_tag="KPB88_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG0054659.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3774..4226)
                     /locus_tag="KPB88_002391"
     CDS             complement(3774..4226)
                     /locus_tag="KPB88_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG0054660.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4674..6032
                     /locus_tag="KPB88_002392"
     CDS             4674..6032
                     /locus_tag="KPB88_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0054661.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6305..6685
                     /locus_tag="KPB88_002393"
     CDS             6305..6685
                     /locus_tag="KPB88_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0054662.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6810..7010
                     /locus_tag="KPB88_002394"
     CDS             6810..7010
                     /locus_tag="KPB88_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054663.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7206..7934
                     /locus_tag="KPB88_002395"
     CDS             7206..7934
                     /locus_tag="KPB88_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0054664.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7956..8873
                     /locus_tag="KPB88_002396"
     CDS             7956..8873
                     /locus_tag="KPB88_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG0054665.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            9015..10199
                     /locus_tag="KPB88_002397"
     CDS             9015..10199
                     /locus_tag="KPB88_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0054666.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10306..10494
                     /locus_tag="KPB88_002398"
     CDS             10306..10494
                     /locus_tag="KPB88_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0054667.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10643..11293
                     /locus_tag="KPB88_002399"
     CDS             10643..11293
                     /locus_tag="KPB88_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0054668.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            11396..12334
                     /locus_tag="KPB88_002400"
     CDS             11396..12334
                     /locus_tag="KPB88_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0054669.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12360..13211
                     /locus_tag="KPB88_002401"
     CDS             12360..13211
                     /locus_tag="KPB88_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0054670.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYIYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5297 a         1278 c         2332 g         4338 t
ORIGIN      
        1 aaaattacta taaaaaatga aatatattaa acaatcaact gaaaaatgaa atgttaataa
       61 aaaaaaattc aaattataaa tattaaatga atataattaa aacaaatata taaatttaag
      121 tataacactc acattttaat gcattgtgaa atttttgttg aactttatgt accataactt
      181 ataaaattta tgtcatgtta tatttgaata agtaataaaa ttgtagatgt aaattaatct
      241 acaaaattta tatgaaatgg agttgatgct atgacagaat taatgattat aagtgggtta
      301 gtactattga tttgtataac ttctagcaag gtattgtata aatttggagt accaatatta
      361 ttgattttta tcttactagg gatgttattt gggtctgatg ggatggtagg aatctatttt
      421 gataactatg aactaacaaa acaattatgt tctattggac taatatttat aatgtttttt
      481 ggagggtttg gaacaaactg gaatatggct aaacctgttg ctataccttc tatattaatg
      541 tcttcactgg gtgtaataat tacggctggg gttacaggta tgttttgcca catggtaata
      601 ggaactagtt tacttgaagg tttattaatt ggggcaattg cagcttcaac agatgctgca
      661 tctgtgtttg ctgtgttgcg ttctcaaaaa ctaaacttaa agggttcact tgcatcatta
      721 ttagaagttg aaagtggaag taatgaccca atagcgtata tgttgacact aattcttttg
      781 acattgatga ataattctgg ggtagaactt attgcaccaa tggtaataaa acaaatagct
      841 tttgggttag ctataggatt tatactagca aaattatctg tttatatttt aagacattca
      901 aacttcgaga tagaaggatt ttatactata tttataacag ctattgcaat actttcttat
      961 gcatttagcg agtatcttgg aggaaatgga tatttaagtg tttacatttc aggtattatt
     1021 ataggaaata gtaggatacc tcttaaaaaa agtttggttc atttctttga tggaatttca
     1081 tggattatgc aaataatgtt attctttatg ttaggattgt tatctttccc ttctaagtta
     1141 ccaaatgtat ttggtatagc aatggccata tctatattca tgatagttat tgcaagacca
     1201 ttagcaacat ttatagttct agcaaaattt aaattctcaa ataaagaaaa attattcata
     1261 tcgtgggtag gtcttagagg tgcggcttca gtagtttttg caatttatgc tgttactcaa
     1321 ggagtagcca tagagaatga catattccat ataatatttt ttatagcgtt gctttcagta
     1381 ggaatacaag gttcattgat accaactgta gccagaaagt tggatttagt tgatgataat
     1441 acacctgtat taaaaacatt taatgattat gatggagaaa ttagctctaa acttatcgaa
     1501 gtaaatgttg atgagggtaa taaatgggct aataagagta taatagattc taatatacca
     1561 gacgatatat taatagttat gataaaaaga aaggaacagg ttttaatacc taaaggttca
     1621 accattataa aaacagggga tattttggta ttgagtggag atagaattga agaattaata
     1681 caattataat ttattttgta ttgactttaa cctaagggga aagtttaaac ttaaattaat
     1741 caagtctaat gcgcaggtga tgagatgttt tctataggaa tgttttctaa aataaataaa
     1801 ataactacaa agacattaag gtattatgaa gatatagggt tgttaaaacc agaatatgta
     1861 gatgaattta caaaatatag gtattataca acagagcaat tacctaaatt acatcagata
     1921 ataactttaa aacaaatggg tttatcatta gatgagataa aaaaagctgt agaaaatcct
     1981 gaagaagttg aaaatatttt aatgaataaa gaaaaagaaa tgttggatgt aattaaaagt
     2041 gaagaatata aaataattaa actacgtaat taccttatga atttgaaagg agaagtcaat
     2101 atgaatgatg taattattaa gtctttacca aaagttaaag ttgcttctat gagaaaagtg
     2161 ttaaaggggt atgatgaact ttttaatcta tttccaaatg taatggctga agaaatgatg
     2221 aaaacagggt gtacatgtgc agttcctgat tattgtttta atatatatca tgatggtgaa
     2281 tatagagaga ctgatatgga tgttgaaatg tgtcaagcta taaatgattt aaaggaagat
     2341 acggatatat taaaatttaa agaattagat gcagttgaaa atgcagtttg tatattacac
     2401 aatggtccat attcaacatt aggaaaaact tacgcagatg catttaaatg gatagaggac
     2461 aataaatata aagtggttgg aaatcctaga gaatcttata tagatggtat atggaataaa
     2521 gagagtgaag aagactggtt gacagaagtt cagataccag tgaaacgcat gttataatat
     2581 gtctacaaag tataaataag aataaaaaag gtggtgtctc aaagtaaaca ttttagttta
     2641 tgagacactc tttttcatat gaaatcaagt atatttttat aattacgatt atgaaaaaag
     2701 gcttatctat atttttagac agcctcttat ttttttatta taatagtttt ctaaagatgc
     2761 tctatttata aatttttatt aaagacttta taaataattg tatttaatat acaaaatata
     2821 tataaatatt gatgttttat atataaagtt gtatattttt ttaaggatat aaagtaaaaa
     2881 aatatacaat atcaagaaaa aaagatattg ggtatttaaa gtaatacttt atttaatttt
     2941 aagtaaatcg ggtatctaca tatatagata ctaatttcaa agaataggag ggtgtttata
     3001 tggaaatatt cgcaatagga cattcaaatt atccatatga taagcttatt aatatgataa
     3061 aaaaatatga cataaattgt gtagttgaca ttagagaaac tccatattct aaatataata
     3121 ttcaatacaa taaagaggca tttaacgaat ctcttagaaa ttccggattt ttatatatat
     3181 acatgggaaa agaatttgga gctaaaagaa ctaataaaga tgtatatacg caagaaggtt
     3241 atgcagattt tgagaaagta gcaaaagaag atattttttt aaacggaata gaaagattaa
     3301 aaaagggttg tcaaatgggt tataggatag ttttattagg agctatgcaa gaacctataa
     3361 gatgtcatag aagtatcttg gtgggaaaag tattaaataa agaaggtttt gatgtaaaat
     3421 atataatgca tgaaggaaat ttggcatatc aggaagatat agaggaatca ttattggata
     3481 agtattttag tgatagaaag caactgagta ttgacaattt attagggagt gcattaacga
     3541 gagaagaaat gatacaagaa ggttataagc tagctaataa agaaatagga tatagaactg
     3601 agaaactagg taaataaaaa tatacaagta gataaaatac agtacttatc ttaagtaaaa
     3661 taatattatg aatttataat agctaggcat gatgtgccaa ttaaaaatat tctagttatt
     3721 taataatatc attaaattta gggtattttt taatagcaca tcatgttgca gttttaaaaa
     3781 ataaaatctc taatattgcc ataatcaact gttctattaa gagtaggatg actagatata
     3841 aatctaactg taatttttga atttaacaaa caaggatttg aaattaaaaa tactctatct
     3901 ccattgacaa tagaagtaat agctactggc aaaatatcta caaatgtatc atatctgttt
     3961 ttagcaattt gataaggata atttcctaca aaaacaaata gatttaaatc atatatatta
     4021 tcagtaaacc aagcccattg accataaact ttgtcgatat tcattgtgcc taaaccaagt
     4081 gtatcaaagt atgttgctat agttaaataa atattttcca tgttttctaa atatgaaaca
     4141 gtatatcttc tgaaacgtat gggtgctgtg gaagaaatag gataaaccat atttacattt
     4201 agattatttg catttaaatt aaccaaagta tcacatccta aaattagtct ataaaataca
     4261 atatttaaat ggatgcgatt ttgttacatc taaagtgtaa tattttatat attgaaaaag
     4321 tggtaaaagt ttggatgatt aaataatatt ataaaaaata tataaaatgt acttattgtt
     4381 attgctacac gatagtgata caatattaac gaaatatagc tacttttaat aataacggaa
     4441 aatatttata ttaaattaat aaatatttta aatgtggtat ccataaaaaa taataaacat
     4501 aagataatta taaagatata ctgtgttcaa atgatattaa acaagattta tattatttga
     4561 aagcagtata ttataatgcg aacaatacaa ttctaaactt tgcattgtca atttacagtt
     4621 cgataaacta attaaaaatt aaatttatat aaagagggaa aagaggaata gggatgttta
     4681 taaggaaaaa tgttataaag ttagcaattc ctataatgat agagcagacg tttgtcatgc
     4741 ttttaggagt atttaataca atgatggcag gtcatattgg ggaagaagct gtatctgcag
     4801 ttggaatggt tgattcaata aataatatat ttatatctgt atttgctgca ctatctgttg
     4861 gagctactgt tgttgtagct caacaaatag gaaaaaaaga tagtgaaaag gtgaatgaaa
     4921 ctattaagca ggctctagtg tctggagtaa tattatcctt tacaataaca atattgatgt
     4981 gggtttttag aactggtgta attaatttat tttatggttc cgcagaagaa ttggtaaaat
     5041 caaatgcaaa attatacata gagtttacat tatttacgta tccatttatt gcgacacaac
     5101 aaatagcaaa tggtatttta agagggtgtg gagatgtaaa gaaacctatg tatgtgacta
     5161 tgtttatgaa tatagtaaat gtaacattag gatatatact tatatatggt attgatttaa
     5221 atcctctagg aataaatttg caaacaccat cctatggcat aacaggtgca gctatatcta
     5281 ttgctatagc tagaataatt ggatgtatag taattataag agctttattt aaaggaaacg
     5341 aatttatatc tatgggtaaa atatttccat ttaagattga tgttgaaact caaaagtcta
     5401 tatttaatat aggaatacca gctggagttg aacaactact ttttaatgca ggtaaattga
     5461 ttgttcaagt atttatagtt actatgggaa catcagcaat tgcatccaat gcaataggaa
     5521 tgtcaattgc atccataata aatgtaattg ggaatgcttt agctttgtct gctacaacct
     5581 tggtgggaca gtatatagga cgtggagata ttaaaggagc taagagtact ttattatatt
     5641 taactaaatt tgcaacaatt tgtttattta tagtaggagc tatatttata ccaattgcac
     5701 cctggatttc aagtatgtat actcaaaatg ctagtgtaat tgacatctca acacaattaa
     5761 ttcgaagtga ttgtttagct atggtaatat ggcctatatc ctttgtatta tcagcagggc
     5821 taaagggagc cggtgatact agatatacaa tgatgactgc agtaataggt atgtggattt
     5881 ttagaatttt tacaggttac gtattggggg ttgtattaga atttggagtg ttaggaattt
     5941 ggataggtat gtatactgat tggcttgtta gagggacttt atattgttta agattaagag
     6001 gaacaaagtg gctaaaacac aaagttgcat agaatatatg aaatcacttt tagatggata
     6061 actctattta gaagtgattt tttatgtagt tattatcata attataagta tgatatataa
     6121 ggtgattaaa ttacataaat aaaggctata atagtatgga tattataaat tatatattga
     6181 gcagaattac ataatttggc gataaatatt agaaatatat atctatagaa aataatatat
     6241 atgggtatat tattaaatat aaaaaatatt aggtatttat ttaataataa aggtgggtat
     6301 tgttatgata aataaaatag gaaaaataac tctctatgta aataatcaag aggatgcaaa
     6361 gttattttgg acagaaaaat tgaactttgt agtaacttat gaaaaggaaa tgggtccaaa
     6421 tatgaaatgg gtggaagtag caccaagtaa aaatgaattt acaacatttg tgttgtatga
     6481 taaaaatcta atgatgaagc aaaacaaaga gataaatgta tctcatccat caataatttt
     6541 aagtacaaga gatattaatg gaacttatga acaaatggca aataacgagg ttgaggttgg
     6601 ggaattaatg gagatgccat atggaaggat gttttctttt aaagaccaag atggaaatga
     6661 ttatttggtc agagaggata aataatttta caaagtatta aatttactca atatctgata
     6721 taattagaga caataattat tataaactag tctaggatta gaattatata attaaaaatt
     6781 aagtgtaaat tttgagtaga ggtgataaaa tgtttaagaa aatggcagta ctaaaagata
     6841 tagcaactaa aataggtcgt aaaaaagcgt atgaactatt agaaatggtt gaaggtaatg
     6901 atgcctttgt agctgaggta aagataaaaa agaatggaat agaatctaaa aaagaagaaa
     6961 ttatgttaaa agataatcaa aaaataatat tagagtatat agaaggttaa attttaaata
     7021 catataagtt gatatactaa atttctgaga tgagtttaca taaaactcat cttttttact
     7081 tttcaataac tttgttttat tatttaatat acattttatc tccttagagt tgctgatata
     7141 taaaaattac tgatataata aaagaatgga tattaatgta atattttata gaaaagaggg
     7201 gtattatgct tgaaataaaa aatctttcct ttaatgttga gtctaacaat gaggaactag
     7261 gaataataaa tgatgttagc ctttcatttg agaggggaaa acttattgtt atcacaggtc
     7321 caaatggtgg aggaaaatct acaattgcta agcttattat gggaattgaa aaagctacga
     7381 gtggtcagat aattttagat ggagaagata taacaaatct tagtattaca gaaagagcaa
     7441 aaaaaggaat aggatacgca tttcaacaac caccaagaat aaaaggtatg acagttgaaa
     7501 atttacttac acttgctcat ggaaaaccat tgtctactga tgtatgttgc caatacctta
     7561 cagatgttgg attgtgttca aaagactatt taaatcgtga agttgataac tctctttctg
     7621 gaggggaaat gaaaagaatt gagatagcaa ctctttttgc tagagattta aaggtttcta
     7681 tatttgatga acctgaagca gggatagacc tttggagctt tggaaaactc aatgaaagtt
     7741 ttaagaaaat acatgaagag tctaatcaga ctataatcat aatttctcac caagaaagaa
     7801 tattagagtt agctgatgaa ataattgttc tacaagatgg ttcagtaaaa agccatggaa
     7861 caaaagaggc tatattacct gaaatcatgt gtcaagttaa ttcaagttgt gaattaatga
     7921 aagacatgaa ttaattagaa aggaaggcaa attaaatgaa caaaatagat ttaaatcttt
     7981 tagaaaaagt tgctgaactt cgtggaactc cattaggagc atacaatata cgtaaaaatg
     8041 ggcaaggagt agagagacat tcaactgaaa atgtaactat aattcctaaa actgataaac
     8101 ctggaataga tattatagta aagccaaata caaaaggaga aaatgtacat ataccagtaa
     8161 tagtaacaga ggctggtgta caggatatgg tatataatga ttttgaaatt ggagaaaatg
     8221 cagatgttgt tataattgct gggtgtggta tacataactg tggttgtgat gatagtgaac
     8281 ataatggtat acatagattt ttcttacgta aaggagctcg cctaaaatat gtagaaaaac
     8341 attatggtga aggtgatgga gaaggaaaga gaattttaaa tccagttact attgtcaata
     8401 tggatgagga ttcatattgt gaaatggaaa caattcaaat taagggtgta gattcaacta
     8461 aaagagaaat ggatgcaaac ctaggtaaag gagctaaaat agttgtattt gagaaattat
     8521 taactcatgg taatcaagtt gcagaatcta atatgaaggt tatcttagag ggtgaaaact
     8581 cttctgctca agtagtttct cgtacagttg ggcaagataa ttctaagcaa aagtttaatc
     8641 cttgtgttat tggggattcc ctttgttctg cacatgttca atgtgattca ataattatgg
     8701 gaaattctca aattagtgct atacctgaaa ttgctgcaaa tcatccagat gctcttttag
     8761 tacatgaagc agctattgga aagattgcag gagaccaaat actaaagatg atgacattag
     8821 gtcttactga agaagaagcc gagcaacaaa tacttaattg cttcttaaag taaattaaag
     8881 attttattta atttaatata attaatatag tataatatag tgaataagtc taaaatattt
     8941 ttatttttag gcttattttc tttatgtaag aaaagctatt agagaaatta tattgtaatt
     9001 aggatgtgga ataaatgaaa aatttactta taagaaatgg gacaataata tctccaacag
     9061 atggtatcaa atttgaaaaa agagatatct tggttttaca tgggaaaata tatgctattg
     9121 gattgaacct taaagagaaa ataagaaaag aaaaattact tacaaataaa gagttcgaga
     9181 taataaatgc cgataatatg tatatttccc caggatttat agatgttcac acccattgtt
     9241 ataagagaaa attacctaca ggtatggatt ctgattcaat tggaattgaa aaaggttcta
     9301 cagttatatt tgatgcaggt acagctggtc cttcaaacta ctatgatttt aaaaaaaagt
     9361 atatggatga gtgtaaaaca gaagtatatt cttttttaaa tgttacaaat gagggactaa
     9421 atatattgaa tgaatcaact tctttagatg ttataaattt tgacaaggtt gagtcagttg
     9481 tagaaaaaaa tatagaaaag ataaagggta taaaagtaaa agcgagtaaa tttcaatcta
     9541 atgaaagtgg tatagatata attaagaaat caaagcaagt tgctaaaaaa ttaaatttac
     9601 ccttagtggt atatatagga gagtatccat catatataga tgaagtaatg aatattttgg
     9661 gaaaaggaga tgtggttaca ccagtatatg gaaatagtac aaatggaatc cttaaagaat
     9721 ctggcacatt aagaaaatca gtaataaatg caaagaatag aggagtattg ttcgatgtat
     9781 gtcatggagt ggaccaattt gattttaaag tttttaaaaa agcaataaag caaggtttag
     9841 agcctgacct aataagtact gatatgcata taaaaaacat gaaagacgta aactattctc
     9901 ttttaaatgt tataaataaa attatggaac ttgggatatc cctagaaaag tgtatagaaa
     9961 aagtaactga atacccaaca aagattttcg atttacatgg aaataaaggc agaataaaga
    10021 ttggtggaga aggtgatttt acaatattca caatggagga gtgtaatgat attataaaag
    10081 actacaataa taatgaactt gttttaaata aaaaattaag attgcaatat actgtaaaat
    10141 catggatgaa aagcagtgaa gtttatagac atggatacga aaatactata ataaactaaa
    10201 caatagtgat aaaataataa atatatgttt aaattggtat atattggata aatatctaag
    10261 gaaacaacaa ttaaaaaata ataattttag gggaggtatt ttagtatgaa agcatttgtt
    10321 gataaggatg tatgtgtagc ttgtggagca tgtataggaa catgtccaga cgtttttagt
    10381 atgagcgatg aagggcattc agtagcaatt gaaggagaaa taccagcaga tttacaagat
    10441 ttatgtaaag aagccgaaga aggatgtcca gtttcagcta taacagttaa atagagctgt
    10501 ttaaatttat ttttaaaata aatatataag atttaattgg tacttaagct atagtattag
    10561 aaatttctat tactatagct ttttaaaata gataaactta atatataaga tattactttt
    10621 tacaaatatg gaggactgaa atatgaaaaa aaattatgaa atagtattat tcgatttaga
    10681 tggaacactt acagacccta aagaaggaat aacaaagtct attcagtatt ctttaaatag
    10741 ttttggcata aaagaagatt tggaaaactt agaccaattt ataggaccac cattacatga
    10801 tactttcaag gagtattata aatttgaaga taaaaaagct aaagaagctg ttgaaaagta
    10861 tagagaatat tttgctgata aagggatatt tgaaaataaa atttatgaga atatgaaaga
    10921 gatattagaa atgctctata aaaatggtaa aatattatta gtagcaactt ctaagccaac
    10981 agtttttgca gaaactatac ttagatattt tgatatagat agatatttta aatatatagc
    11041 aggtagcaat cttgatggta caagagtgaa taaaaatgaa gttatacaat atgtactaga
    11101 cctatgtaat gtaaaagata aagataaagt tattatggtt ggagatagaa agtatgacat
    11161 aattggagct aaaaaaatag gtatagattc tattggagta ttgtatgggt atggttcttt
    11221 tgaagagatt agtgaaagtg aacctactta tattgtcgaa aatgtagagt ctataaaaga
    11281 tattttgtta taaatatctt ttatagacaa aaatattaca aaatggtgtc gcatatttaa
    11341 aaatatgata ttatagttaa ggaagtcatt taaaaaagag gaggatgcac ttaaaatgaa
    11401 atctatttta attatgggtg gaagcgactt cataggttct gcacttgcta agcgcttgat
    11461 taaatgtggg tatcaaattg atatacttac aaatggtaaa aaggaaatag attataatgg
    11521 ttttaaagaa catctaattt gtgatagaaa agttcgaaaa gatatggaaa acattatcac
    11581 aggaagaaaa tatgattaca tatatgatat gacagcatat acaaaagaag atgtaagtaa
    11641 tctgattgat tttataagca tggataactt aaaaaagtat atagtattgt cagcaggagc
    11701 agtatataaa gatagtggaa gaaatataaa agaagaaaat gagaaaggtg aaaatgaaaa
    11761 ctggggaaaa tatgggttaa ataaaaaaga ggctgaagat tttattataa atagccctat
    11821 accatatata attattagac caacttacat atatggtgaa aataataatt tatatagaga
    11881 gtattatttc tttgaaaaaa tagaaaaaaa tgaaaagata ccagtaccaa aaggaaaaca
    11941 agttagtaat caatttatat atattgggga tttggtaaag gtattggaaa gcataatgaa
    12001 aaatcctcat gtaagagagg cctataatgt gacaaatcca cagttaattt cttgggatga
    12061 ccttatatat acatgtggag aaattattgg caaagaacct ataataaaat atgtagatat
    12121 ggaaaaagta gagtttagag aaagaacgta tttcccattt agaaatatag actttaatct
    12181 tgatataaat aagcttattg aacatgggct ttatatacca aacgtgttac taaaagaagg
    12241 tcttacagca acatataaat ggttttcagc aaataagcca aaaatgcatg acagaaaaat
    12301 gaacaaagtt gagtctgtaa tgcaaatagt ttaaaaaaat aaataagtag gtagatattt
    12361 tgaactcaaa aattaagtat tttttaaaat tttttatctt aatatttatt ttgattttta
    12421 tatactcagt tgcgatagag ccgagtctat taattgtgaa aaaatataat ataagtgaaa
    12481 atggagcatt gataaaaaat gattcaattg agagtaaatc tgaaagtgta aaagtagttc
    12541 aaatttcaga tactcaaata ggtagttttt attcaactaa gaatctcaaa aaagtagcta
    12601 ataagattaa cactttgaat ccagatataa tagtatttac tggtgattta attgattact
    12661 ccaataaaaa tccaagtgta gatgaaatta caactatttt atcaagtatg aatgctaggt
    12721 tgggaaaatt ttcagtattt ggcaatcatg attatatata taaacttcct aggtattata
    12781 ggcagattat gaaaaattcc aacttcaatc ttttagtaaa tgaaaataaa aaaataaaat
    12841 taaaagatga taaatatatt aatatattag gtgttgatga aatattaaat ggaaatccta
    12901 atataaaata tctggagagt caaatagata ataaaagttt taatttattg ttagcacatg
    12961 aaccagattt agtagatatg cttagtaaag atacaatgaa tttagttcta tctgggcata
    13021 gtcatggtgg tcaaataaga ttacctatta agggagcatt agttactcct ccatatggaa
    13081 ggaaatatac taaagggttt tatgatataa atggaaatca tttatatgtg agttcgggtc
    13141 ttggaagtac aaagttacca tttagatttt ttaatatacc agagataata gaatttaata
    13201 ttataatcta acataaaata tttagaaaat ataattttgc ttttt
//
LOCUS       DAELJO010000103        13216 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELJO010000103 DAELJO010000000
VERSION     DAELJO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.103"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(114..857)
                     /locus_tag="KPB88_002402"
     CDS             complement(114..857)
                     /locus_tag="KPB88_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0054671.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYSKKLINHFHQKDKLLDVDYTNI"
     gene            1023..3590
                     /locus_tag="KPB88_002403"
     CDS             1023..3590
                     /locus_tag="KPB88_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054672.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3820..4224
                     /locus_tag="KPB88_002404"
     CDS             3820..4224
                     /locus_tag="KPB88_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054673.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4248..4745
                     /locus_tag="KPB88_002405"
     CDS             4248..4745
                     /locus_tag="KPB88_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054674.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4748..5149
                     /locus_tag="KPB88_002406"
     CDS             4748..5149
                     /locus_tag="KPB88_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0054675.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5167..5655
                     /locus_tag="KPB88_002407"
     CDS             5167..5655
                     /locus_tag="KPB88_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054676.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5682..6437
                     /locus_tag="KPB88_002408"
     CDS             5682..6437
                     /locus_tag="KPB88_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054677.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6437..7300
                     /locus_tag="KPB88_002409"
     CDS             6437..7300
                     /locus_tag="KPB88_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0054678.1"
                     /translation="MQEIKNKEVKNGQDPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7347..7826
                     /locus_tag="KPB88_002410"
     CDS             7347..7826
                     /locus_tag="KPB88_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG0054679.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLAKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7891..9198
                     /locus_tag="KPB88_002411"
     CDS             7891..9198
                     /locus_tag="KPB88_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0054680.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9508..9648
                     /locus_tag="KPB88_002412"
     CDS             9508..9648
                     /locus_tag="KPB88_002412"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG0054681.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            10158..11252
                     /locus_tag="KPB88_002413"
     CDS             10158..11252
                     /locus_tag="KPB88_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054682.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11257..11457
                     /locus_tag="KPB88_002414"
     CDS             11257..11457
                     /locus_tag="KPB88_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG0054683.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11457..12374
                     /locus_tag="KPB88_002415"
     CDS             11457..12374
                     /locus_tag="KPB88_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054684.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12371..13129
                     /locus_tag="KPB88_002416"
     CDS             12371..13129
                     /locus_tag="KPB88_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0054685.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVTTLFLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
BASE COUNT         5070 a         1391 c         2442 g         4313 t
ORIGIN      
        1 cataaaagta attaagcttt attaaatagc ttctttatta taatttcttt atttcatata
       61 atataaatag tattaaaatt tttatgggag ttaaagtaag catgtattat tacttagata
      121 tttgtataat caacatctag taatttatct ttttggtgaa aatgattaat aagtttttta
      181 gaataattca ttattaattc atctggaaaa ttaatttcct ttaagatatt ttcagaatac
      241 tcatagttac ctatgtcaaa acaaatgtga gcatctgaag ttagtatgat tttagcacca
      301 tattttttgc aaagtgtagc aatatactta cagttatccg agctaccatt tcttgacacg
      361 cctttaatag aacaattatt tatttctatt aagatattct tttctatagc taatttaatg
      421 attaattcat agtcgattgg gtagtttggg tttccaaggt gtcctaatat ttcaatatta
      481 tcatgcttat ttattgcatt tattaaagct ttagtattat tctctaatgt atttggagaa
      541 aacacagctt cgtgaaatga caagataaga taatctaacc gaggaattac aaaaggctct
      601 aggtctatgt ttcctttaat atctagtata tttgcttcac agcctcttaa aattataatg
      661 ttatttatga ttcttgggat attttgaatg ttgtgaaagt accatctgtg gggagaacca
      721 ggcatcttag gaccatgttc agatgtacct agtatcttaa ttccattttc tttacaataa
      781 ttaatatttt ctagtaaagt gctatagcca tgaccactga ctatagagtg ggtgtgtaaa
      841 tctgataaaa aattcatata aattcctcct ctataaaaga tgagggtaac gtaatttata
      901 ttataaaacg aataggaaag aaaatcaata gttacataat tagacaaaaa tattaaaata
      961 ttgtagaatt aagcttaata ataaatgttt aacagtatta ctgcttatag gggaggttaa
     1021 ctatgacaca aaagaagcta tcaaggaaag aaaaagtttt aatttgcttg aaaaggttaa
     1081 cagaagaaca cttgaacttt aataatggca ttagatttgg atttgatgca gaatatatag
     1141 ctactgaagt aggtataaat agaagtaatg ctagtaagga attaaatcaa ttaataaaag
     1201 aaagtattat tataaagata aagggtaagc ctgttcagta cttatataaa gaaagtattg
     1261 agagtatttt aggaaagaaa ctaaataaag ggatatttga taatattaac tctataaacg
     1321 aatatagtga aaatgattct atcaaagatg agtatatttt agagagtata aaaaaagaga
     1381 acaatatttt taatttagta ggatgtgatg gaagtttaag gtcccaaata gaacaggcta
     1441 aagcagcagt aatatatcca cctaaggggt taagtacatt aataatagga cctacaggag
     1501 ttggtaaatc tatatttgca gaatatatgt ataagtatag tttaactata aaagaagata
     1561 gtgataatgc accatttatc atatttaact gtgctgatta ttctgataat cagcaattat
     1621 tattagcgca gctttttgga tatgtaaaag gagcatttac tggagctgat aaagataagt
     1681 atggtattgt acatgaagct aataatggag tattgttttt agatgaagtt catagattat
     1741 ctgcagaggg acaagaaatg ctatttctgc ttatggataa agggatatat agaaggttag
     1801 gagaggctaa taaggtacac gagtgtagag ttatgataat tgcagccact acagaagacc
     1861 cagaaacagc aatgttggaa acttttttaa gaagaatacc tgtaactata aagattccgt
     1921 ctttagagga aagaggcttt caagagagaa tgaagcttat atgttatttt tttaaagaag
     1981 agagtgttag gattggtgtt aagttaaagg tttcaaaaga agttattaag gcatttattc
     2041 tatataagtg taagggaaac ataggccaat taaaaagcga tatacaatta atttgtgcaa
     2101 gagctttttt agattatatg acctataaaa gggaatgtgt atatgtaagg ctctctcttc
     2161 taccaaataa tattagagaa tcattatatg gaaataatag aaaagaggag tttgtacaaa
     2221 atttcagttt tattggaaaa gatgatatta tttttttacc agaagaaaag gattttgaaa
     2281 gtgaaaattt attaatagaa aataaaaaat atgatttaga tttttatgga atgataaaag
     2341 aaacttggaa taaacttcaa caagaaggta ttaaagagtc acaaattaga agtgttatag
     2401 atggagacat acagaagtat tcctataatt taatgaatac ttttatatat aatagtagta
     2461 atcaaacagc ttataacaac attgttaatg attcaattgc aactactgta aaatatattt
     2521 tacaaaagca tgataaatgg agcaaaagag aaggattaga taaattaata aaagctatag
     2581 ctttacatat tcagaattta attgaaagaa taaagattgg aaatatagta aagcatccta
     2641 ataaagatga aattatgaag gaaagagcat atgaatataa catagcaaaa gaaatattat
     2701 ctaacatgtc attaatgtat ggagtagagt ttccagaaga tgaagcaatt tttttagcaa
     2761 catttcttta tttatcgtat gtgggattaa atgaggagag tatagcaata ttagtcataa
     2821 tgcatggtga atctacagca tctagcatgg tgaatgtagc caatacttta ctggattgta
     2881 accatgcagt ggcaattaat atgggattag aagatagagt tcaggatatt ttagtacaag
     2941 ctgtagaaat agccaataaa attgataaag gaaaaggaat tttaatttta actgatatgg
     3001 ggtctatttt aacatttgct aaagttattc gtgatgctac aggaaaagaa gtaaaagcaa
     3061 tagatatggt aagtacacct attgtaatag aagcaactag aaaggctcta actccagaaa
     3121 tgacattaga gaaattatat tttaatatta tagaatctat aaagaaacat tatggagata
     3181 tggaagttac atatgctgaa aaagataatg gtggtagata ttttgataga ttactaattg
     3241 ataatattag taaaactttg acttttttaa atggggaaaa ggcttatttt atattaaaag
     3301 aagtaattaa tagaattagt gaacactatt ctttagtaat tcaagatgaa cttttagtaa
     3361 agtttatatt tcattgttca tgtatgattg aaagagtaat aataaaagag tctttagttt
     3421 ataaatcata tgaagaaaga attagtagaa ataaagaatt atatttagtt ataaaagaaa
     3481 gttttaagct agtagaagag acattcgata taattatttc agatatggaa tatgcaaata
     3541 tattagatat atttgaatca caatatgata cagataaatt caaaagttga tacaaaattt
     3601 aaaaatagcc aaaaggctat ctttttttta ctttttttga tacagatttg atacaggttg
     3661 aaaaaatctt ttgatacata tcgaaataat aagtttttaa aaaaaattga gtaaaaagtt
     3721 ttgattaaat cctgtaaaat agccatttgt tattgataca cataagttgg cacggcaatt
     3781 gcttatataa aaagtgacaa gaaaagtgag gtgaaattgg tgataggaat aatagttatg
     3841 actcatggga gttttagtga agaaattata aaaagctgtg aacttattgc aggaccagca
     3901 gaaagaacag cagcaattaa actaaatagg aatgataata ttgaagactt aaataaaaat
     3961 tttgtagaaa agttaaatga attagatgaa ggtgacggag ttttagtatt agctgaccta
     4021 ttaggaggtt cgccaagtaa tgttgcaagt ttaaacctta aaaaaggtgg caagtttcat
     4081 gcacttactg gagtaaatct tccaatgcta cttgaagctt taattaatag agagggaaaa
     4141 agtttagaag agttagcaga agcatgtatt gaagcaggac aaactggtat aaacaatatt
     4201 aacaaagttc ttagctcaat gtaatagaaa aaggagtttg attaaaaatg tctttagtaa
     4261 aatattgcag atgtgatgat agattaatac atggtcaagt tatatataaa tgggttaagc
     4321 atctaggtgt aaaaaagata gtagtagtag atgatgaaac aaccaatgat gttattgcta
     4381 aaggtttgat aaaaatggca gcaccaaaga atattgattt atcgatatta actgtaagtg
     4441 aatcaaggag atatttttat aataatcaag ctgatgataa cgtttttgta ttaattaaaa
     4501 atttagatac tgcaaataga atgatagaag aagggattaa tatcaaaaaa cttattgttg
     4561 gaagaatacc tacaggaata ggaaaaaaga aaatatctca aaatgtatac attaataaaa
     4621 aagagtttct tttaatagat gaatttatta aaaagaatat taatgtatca attcaaatgg
     4681 tacctgatga agaagaagta gaattaaacc aaaatattaa tcgctataaa ggagagttta
     4741 tttagcaatg aataaatata aggtaatcat actgtgccat ggaacttggg gaaaagtctt
     4801 agtagaagaa acaaataaaa actttggatt gtcaaatcaa tatgaagttt tatcactaag
     4861 ttctgaaaaa gatgtttctg tatttatgaa agaggttgaa gaagtagtag ataaagaaga
     4921 tgtgaaaatt ataatatcag atttatatgg aggatctaca tcaagtgtcg caatagctgt
     4981 tgctataaga agaggaatag atgcaataaa tggacttagc ttacagacaa ttttaatcgt
     5041 agatgaagaa ttaagcaaag aagataaatt acatacatta ccagaaagaa tcgtcaaaaa
     5101 aaataataac ctttgtgtgg atttaattaa agaatttaaa aaaatataaa ttatgaggtg
     5161 aatataatgg caaaaataac attagtaaga gtagaccaca gattaattca tgggcaagtt
     5221 ataacaaaat gggtaaaaat cgctcaagct caaaaaataa taatagttga tgacttctta
     5281 ggtcaagatg aatttatggc tgacatatat aaaatggcag caccatcagg agtagaagta
     5341 gttattttaa cagcagaaga tgcgggacaa gcattccaaa acaacacttt aggagataaa
     5401 aatatattta ttctatttaa aaatgttgat atggcaaata aggcttataa agctggactt
     5461 aaatatgaaa agattcaatt aggaggtatt ccaaatgaag caggaaagaa aatggtgttt
     5521 acagctgttt cattaggcaa tgaggatgta gaacaactta atgaacttaa tgaaaatggg
     5581 gttgaaatag tcctacaagt tataccagag gaatcatcta tgacatatga aaatgcgtta
     5641 aagaaattta aataaaaaat ataaattagg aggaattata aatggaatct gttttgatgt
     5701 tggcaattgt aacaggatta tggtattggt ttgcagcagg tcttgcaggg tatacccttt
     5761 tctcaacact aaagtctcct ttatttatag gttttagcct tggattactt tggggtgatg
     5821 taacaactgg tatgattgtt ggtgcaagta tagaaatggt ttatctagga atggtcgcag
     5881 cagggggaaa tattccatca gataaatgtc tagctgctct tattgcaata ccagtagcac
     5941 ttcaaactgg tgttaatgca gaagttgcag tatctattgc ggtaccttta ggtgttatag
     6001 gagtattagt taataactta agaagaactg gtaatgctgt attggttcat aaggcagata
     6061 agtatgctga agaaggtaat acaaaaggca tctggagatg tgcaacactt tattctttaa
     6121 tatttgggtt tgttttaaga tttccaatag tttttgtatg taactttttt ggagcagatt
     6181 tagtccaatc attattagat gttataccac aatggttaat gaatggatta acagtaatgg
     6241 gcggtatatt acctgcatta ggttttgcaa caacaatatt tactataggt aaaaataaat
     6301 tcttaccaat gtttattata ggatttttta tggttcaata ttttgaaata tcaattactg
     6361 cagctgcaat atttggtgtt tgtatagctc ttttaattac ctttatgaaa gaagacaaaa
     6421 gggtaggtga agtataatgc aagaaataaa aaataaagaa gttaaaaatg gacaagatcc
     6481 aactgaagtt aataagacaa gagtacttac aaagaaagat gtaacaaaaa catatctaag
     6541 atggtggtgg acagcagaat tatccaactc ttttgaaaga atgcaagcat tagcagtttg
     6601 tgcaagtttc actccggctt tagaaaagtt atataagaga aaagaagatt tagtagatgc
     6661 gttaaagaga cacttacaat tttttaatac tcaagctata tggggaggat taattcacgg
     6721 tacagtttta gctatggaag aagagaaagc tactgaagga aaaatcccag gagaagttat
     6781 ttcaggtgtt aagaatggtc ttatgggacc tttggctgga attggagata ctttagattt
     6841 tggtacattt caaacaatat tcttagcttt aggtgcttca tttggagcag aaggaagtgt
     6901 tataggtgca tttttcccaa taatgtttag tatattacta ttttgcgaag gttattactt
     6961 atttcattta ggatattctt taggtagaga ttcaattaag aaaattctat caggtggtat
     7021 tgtcaataag ataattgatg gtgcttcaat tctaggaatg tttatgatgg gagcattatc
     7081 tgcaactaca gttaagcttt caactccgct atcatttgat attggtggta aagcaattgt
     7141 tgttcaagat acattaaata tgattgctcc aggtttatta ccacttggtg ttgtattctt
     7201 cgtatattgg ggaatgaaat acaagaagtg gacaataact aagttattag taattttagt
     7261 tgtattagca ttggtagggt cattcattgg aatattctaa ttcacagtga ttaaaatata
     7321 gaatttaatt ttaggaggag aaagaaatgt cagaaaaaat gtatacagaa aaggaatatt
     7381 tagaaggaat tagaggagca acaggagaaa gagcagtatg gttctatctt cttatgaaag
     7441 aagctgaaaa attaggagct aatccagatg atatatgtaa agaagcaata tatggatttg
     7501 gtaaaatgag aggtcaaaaa tataacgtag cagatactcc aggaaaaatg gcagaaatgt
     7561 tatataacag taaaggacaa aaagtgtttg aaatggaatt agtagaaaat acagatgaga
     7621 atggcgtttt aaaattccat cactgtccat tagatgctgc ttggaaagaa tatggattag
     7681 ctaaagaaga aagaaaagaa atttgtagat tagcttgtta tggggattat ggaagagttg
     7741 actgtgcaca aggtgttaaa ttagattttg ctcaaaaatg tgctcatgat gatgaagttt
     7801 gtgaattagt atttacaaaa aaataatttt taatgctaaa agcctgttct ttaaggacag
     7861 gctttaaaaa gatagaaagt aggttgattt atggatataa gcagaagtgt agataatagt
     7921 ttaaatagaa ccatagaatt tctaaaagaa ttgataaaaa ttgattcaca acaaggtgaa
     7981 cctatttcac aatgtccttt tggtatagga cctaaaaaat ctttagataa aactttagat
     8041 tactgtgcta gtttaggttt tagtgttaaa aatattgata attatattgg atatgcagaa
     8101 attggagagg gagaggaatt aatagggatt ccaatgcatt tagatatagt tccaccagga
     8161 gaggggtgga gtgtagaccc attttcggga gcagtaattg acaatattat ttatggaaga
     8221 ggtgtcatag ataataaggg tgctgtatct atgcttatac atgtattgaa aaacatagag
     8281 gatatgtatc ctacaattaa taaaagaata agattaattt ttggtacaaa tgaggagaca
     8341 ggtatgaaat gtatcaaata ttacttagat aaaggtgaag aaattccaag tatgggattt
     8401 actccagatg ctatgtatcc agtggttaat ggagaaaaag gaagggttca tataaggata
     8461 gaaaaagaaa ttaaaattga caaaagtaaa ccttacataa tagtaagggg aggaactaaa
     8521 gaaaatgttg ttccatctca ttgtactgca aaaattatta atggaattat ttcagagtta
     8581 actactaaag gagtagctgt acatgcaagt aatcctgaaa agggagaaaa tgctatatct
     8641 aaaatggtaa taaagatagt tgaggataat atggattttc agcatagaga agatatagaa
     8701 ttagtttcaa aatacctttg tagtgattat tatggagatg cattggggat taatcaatat
     8761 gatgaggtat tcaaaaacac taccttaaat ttaggaatat taaaagtaaa tgaggaaaaa
     8821 atcgtatgtg agttagatat aaggtatggt aaaaatatag ttttaaataa cataatagat
     8881 agattcaaaa aggttttttg taatggttgg aaaatagagg ttattgctca taaagactta
     8941 cattatgtgg atgagagtaa tttagtactt aaaaaattat tagaagccta tgaagaagta
     9001 acagatgaaa atggatatac aattgctatg ggtggtggca catatgcaag ttggtttaaa
     9061 gatatggtgg cgtttggacc taagttctta gcgtataaaa ctggtggtca tggagtagat
     9121 gaaagagtac caataaatca tatacgaaag aatatggaga tatatacttt ggctttaata
     9181 aaactattag aattgtaaaa gaagaagaga ctttatgtgc aaatttacag atatcttaaa
     9241 aagagagata aaacgagttc aaccttattt aaatgttcaa caccatcaaa tggggctgtc
     9301 tcaaaataga gataagcctc ttacttattg ttgaaatgat gaaaatatat tttacttcat
     9361 acaaaaaaga gtgccttatg aagtaaaaag ttaattttga gacaccctca tttgatgatg
     9421 ttgaagagaa aataagtatg aaattattaa aaaagatgat tttataaaag ttaaggttga
     9481 aaagaaaagt taattaaatg gaattagata aatggagaag gatgtatact atctaataaa
     9541 ataacaaaag agtgttataa agttggttat atgattagag aaaagtctat tgatacttat
     9601 ccagatagtg gatggggatt tatgacttgg gatgaaaatg gagaataata aaaattatag
     9661 tttatacagt ttaataagat atatgtacaa gcagttaatc taaatgattg actgcttttc
     9721 ttttatagat ttttatataa tagacaaata ccaatacatg atagttgcct gttataatta
     9781 gaggaataaa tacgaataat aactttataa aagaattaaa tggaaaatcg aagagttata
     9841 ttcagtattt taaaaagaat atgaaatgag aaatgaaaat aaggtgttta tattaaaaga
     9901 ggttttacac aaaatattta aaaacgtgat aaataaaaac tgtatttaga tagattaact
     9961 attagtttta tataatatgg attttattta caaagtttac atgttttaca aaagagacta
    10021 aaaataaatt taaatatata ttttatatac ttgtttattt gtttctctgt ggttgatttt
    10081 ttcgtcaaaa gtcaacttca aatcgcttgt tagctttaca tgtttaagga tataattata
    10141 cataaggagg gattgacatg aatgaattaa acatcgcaaa aacgctgatt ttaaagcgta
    10201 aagaaaaagg aattacacaa gatgaacttg caaattatat aggcgtttct aaagcttctg
    10261 tatctaaatg ggaaactgga caaagctatc ctgacataac ctttttaccg caactcgcga
    10321 catatttcaa cattaccgtt gatgagttaa tatgttatga acctcaaatg atgaaggaag
    10381 atattaataa actgtataat aaactgtgca aagactttac ggttaaacca tttgatgaag
    10441 tcatggatga aataagagaa atcataaaaa aatattattc atgttttcct ttgatttttc
    10501 ggatgggtct tttaatagtt aatcactatg atattgtaga tgaaaaaaag cgtgaactat
    10561 taattagtga agcattagaa atatttattc gtatacagga aacttgtaat gacatagata
    10621 tttgccgtca agcaaaaagt atggaggcaa cttgttatat attgttaaat caacccatac
    10681 aagtgattga tttattacaa aacagcaatt ttcccatgat aaatgaatct atacttcttg
    10741 cacaggggca aatgatgaat ggccaaatgg atgaagcaag agaaacattt caacttggtg
    10801 catatcagaa ccttatatca ttagtccaaa atctagtggg tattttgcaa aatgcagata
    10861 aattacaaat gaaggaaata gaaagacgta ttctagcaat ttcagatata tttgagcttg
    10921 atacattatc tcctgctatt atgctttctt catatttaac acaggcacaa attaatttaa
    10981 tacatggtga taatgaagga gctattaaat cattacgaaa atatgtagat ttagcaacac
    11041 gtgatatata cccaattata atacatgggg atgacttttt taataaactt gacagatgga
    11101 tttctgaaat tggaacaggt attacacgtg atgatacaat cgtaaaagca ggtattgttg
    11161 ctgctattaa aaataatcca atgttttctg tattaagtga aaacaaagaa tacaaatttc
    11221 ttatagaaaa attatcttta ttggaggaat aagattatgg aaaatttgat ggaatactta
    11281 ccacttttgg ttcctgttat tattttagat ttaatactta taattactgc acttgttcat
    11341 gtgttgagac atccaaatta taaaattgga aataaggcaa tatggattat tgtagtactg
    11401 tttattagtc taataggacc aattttgtat tttacaatag gaagaggtga agaatgatga
    11461 atattgtctc aataaatgga ttatcaaaag gttttggtaa tcgaaagata atagataatt
    11521 taaattttac tgtaccagaa gggtcagtct ttgggtttgt aggtaagaat ggtgcaggta
    11581 agactacaac aatgaaaatg gtacttgggt tacttaaacc agatagtgga actatagatg
    11641 tttgtggtga gaaagtgacc tatgggaaaa caagttctaa tcgtcatgta ggttatctgc
    11701 ctgatgttcc agagttttac aattatatga gaccacttga atatctttct ctttgtggtg
    11761 aaattacggg actttcaaaa aaagaaatac agataaggag tgaagagttg ttatcacttg
    11821 tgggtcttgg aaatgaaaag agacgaatag gtgggttttc tcgtggaatg aagcaacgac
    11881 ttggtatagc tcaagctttg ctttcacgcc ccaaattact tatttgtgat gaaccaacaa
    11941 gtgcacttga ccctgttggt cgaaaagaaa tattagatat tatgcttaaa atcaaagact
    12001 caacaactgt aatattttca acacatatac tatcagatgt tgaaagaatc tgtgaccatg
    12061 ttgctatatt aaacaaagga agcattgccc ttagtggtac actttcagaa ataaaaagta
    12121 tgcacggaaa ggatagactt ttactggagt ttgcaagtaa tgatgaaatt caaaagttta
    12181 agtctagtga tggtattaaa tccttactaa aagattcaga agaaactaat atggaaattg
    12241 ttttgcatgg taaagatata aaagcaatac aaaaaacagt tatatctaca ctcgcagaaa
    12301 tgaatctttg tccagtaaag atggaactaa tagaattatc acttgaaaat ttatttttgg
    12361 aggtagtaaa gtgagagaat acatagcatt tacaaaaaaa gaatttaaag agaatttaag
    12421 aaattataaa ttatttagtt taattatttt atttctaata tttggtataa gtagtcctat
    12481 ttctgcaaaa tttatgccag acttgattgc tcattttgct ccgactttaa aagttactgc
    12541 tgcaccaact gctttagatt cttggacaca attttttagc aatatctctg gtcttggtat
    12601 gagtttaact ctaattattt tttgtaatat tttatcaaat gaatattcta aagggacact
    12661 tgttattatg ctgactaaag gtttatcacg ttcatctata gtattgtcta aattctctgt
    12721 tacggtgatt attatgacaa ttggtttttg gttgagtttt ttatttgctt atggctatac
    12781 aatgtatttt tggccaactg caaatcttaa tcatattata ttttcagcat ttaatttatg
    12841 gcttatagga attatgtata ttagtgtatt aattcttggg tgtgtactat ttagaccagc
    12901 atttgccagt gttttacttg tgttggtgac aactcttttc ttgagtttaa tttctattcc
    12961 taaacagatt gcaccttata ctcctaattt cttgatgtca aaaaatatag acttgatttc
    13021 agggaaagtg gcaatgccag aatttattat tccaattatt ataacagttc ttatttctat
    13081 agtatgttta ctagcagctg ttattttatt taataaaaaa ccagtttaat aataccttta
    13141 tcacaaggta aagtaatctt atgttgttaa aaactacacc taaaaatcaa aaagacttgg
    13201 acctaagttt caagtc
//
LOCUS       DAELJO010000104        13189 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELJO010000104 DAELJO010000000
VERSION     DAELJO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.104"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(146..799)
                     /locus_tag="KPB88_002417"
     CDS             complement(146..799)
                     /locus_tag="KPB88_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0054686.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYEMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPMEEMKKEKLL"
     gene            complement(1044..2909)
                     /locus_tag="KPB88_002418"
     CDS             complement(1044..2909)
                     /locus_tag="KPB88_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG0054687.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2914..5334)
                     /locus_tag="KPB88_002419"
     CDS             complement(2914..5334)
                     /locus_tag="KPB88_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG0054688.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5348..6790)
                     /gene="glgA"
                     /locus_tag="KPB88_002420"
     CDS             complement(5348..6790)
                     /gene="glgA"
                     /locus_tag="KPB88_002420"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG0054689.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6819..7949)
                     /gene="glgD"
                     /locus_tag="KPB88_002421"
     CDS             complement(6819..7949)
                     /gene="glgD"
                     /locus_tag="KPB88_002421"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG0054690.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7951..9111)
                     /locus_tag="KPB88_002422"
     CDS             complement(7951..9111)
                     /locus_tag="KPB88_002422"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG0054691.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            complement(9882..10925)
                     /locus_tag="KPB88_002423"
     CDS             complement(9882..10925)
                     /locus_tag="KPB88_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG0054692.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(10946..11392)
                     /locus_tag="KPB88_002424"
     CDS             complement(10946..11392)
                     /locus_tag="KPB88_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG0054693.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVDTKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKADATVNVTNQ"
     gene            complement(11566..13146)
                     /locus_tag="KPB88_002425"
     CDS             complement(11566..13146)
                     /locus_tag="KPB88_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG0054694.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         4163 a         2421 c         1264 g         5341 t
ORIGIN      
        1 ttgatttata tatgttaatt taaatacatt attctatcgt agtgccttag agtttttatt
       61 tgtatttaaa gttaaattta ttcttgtttt tcctttaata tttaatagta aaacaacaaa
      121 attataaaaa aaataaagag aaactttata aaagcttctc ttttttcatc tcttccatag
      181 gtatcgtttt ccaatgttct ctcaaaactc tttcaacatt ttctaaatca ttctccctca
      241 tagattttac tacttcaata tgttgattat tgctgtatgt caacatttca tataactcat
      301 cttcattatc actagtatat gactttgaca taaaattata tcttaaagag ttcaatagat
      361 ttactaacac ctgattatct gatttagtta tgtatatatc gtgaaattca ttacttaatt
      421 ttaaatagct attgtaattt ttatacttta tagagaccgc catcatttcc gttaattctt
      481 ccatttttac taggtctgat tcagttaaat tttgtaatgc tgaagaaccg gctaacgcat
      541 ccaatactcc aattacggaa taggtgtcta atttctcctt ttcctgaaat tcttttacta
      601 caaatccctt attaggtatt ttttctagta aattttcacc tgctaaccta gttaatgctt
      661 ctctcgctgg tgttctacta actccgatag atttacaaat ctcagattca gtaattctct
      721 gattagcttc atattctcca ttttggattt tctttgatat atagtcatat acttgagttg
      781 taagtggatc taccttcata agacgttcct ccatattaag attataaaag ttaaatctcc
      841 ctacaacttt ggtagagagt tcagttttat cttactaatt tatataatat cacaattatg
      901 atatttttta tacttctttg taatattttg aaatactaaa attcttatga taatttatac
      961 aaaaaaagta gaaaaagttt tgataaaaca aaactctttc cacattcacc ttattttcta
     1021 tttatttttc taaaaaaaga tatttaattt actctaaaaa ttctaaatga tttagagtct
     1081 aaatctactt tcaactcatt attttcaatt tgtatagtac tatttgcatt tttaagacta
     1141 tactttatag gtatttcctc aataaaacta ctctctatac ataatgatat actttccaca
     1201 ttttcacttc tatttactaa aattaacatt ctatcattat tttcattaac tctaataaaa
     1261 gaaaatacag aatcattatt agtatatata aattctgtat ttccagaact taaaagttta
     1321 ttcttatttc taatttttat aacatctcta taaaaattaa gcatatcttc gtcttcattt
     1381 ttccatggat aagttcttct attatctggg tcaacacctc cacaaagacc tgcttcatct
     1441 ccataatata tatatggtac tccttcaaat gtcatctgta tagctacagc aagcttcact
     1501 aaatctacat catcatttaa ttccgtttta gctcttgtaa catcatgtgt tccaattaaa
     1561 tttaaattag acctaaatga ttcttttgga taattttctt taatctgcat atatctatta
     1621 caaagttcat atgaattaat ttctcctttt aagaaagaaa acatgttatt tctaaaagga
     1681 taacccataa cagaatctaa ttcttcacct aataaataac ttcttctctg accatagcta
     1741 atcttatttg atgcatcttc ccatacttca cctataagaa ttgagtcatc atctgccttt
     1801 tttaattcct tttttaactc tcttataaac ttagttggta attcgtctgc aacatctagt
     1861 ctccagccct taatacccat atttacccat ttatttataa cgctatcatt gtcatatatt
     1921 atataatcca tatagctatg ttctaattca tttatatttg gcaatgtctt aacatcccac
     1981 caacttttat atttttgagg aaattcttca aacatatacc atgaataata tggagattct
     2041 ttagattgat atgcccctaa actattatag tttccataca tattaaaata tttactatct
     2101 gctccagtat gactaaaaac tccatctaat actattgata tacctttttc ttttgcttta
     2161 tcaataagtt ctttaaatgt atcttcatcc ccaaacattg ggtcaatttt tttatagtca
     2221 cctgtatcat acttatgatt actagaggct tcaaatatag ggcttagata tagtatactt
     2281 acacctaatt tttttaaata tccaagttta ttgattatcc ctcttaaatt tccaccatgg
     2341 aaatcccatc ttataacatc cccttgacta tcctttatgt acataggtgt atcttcccaa
     2401 tttccatata tgaaactatt ttttttagga ttatcaacct taccactacg atttccatta
     2461 ttgaatctat ctacaaatat atgataaagt attccttctt tgtaccatgt tggtacttta
     2521 aaatctttgt aaacagtgat ttgatattta ttaatatcac tataattata ttcacaagaa
     2581 tatccattat ctctgttttt tccataaaat aaagtcttta tctgaccatc catttcaaca
     2641 tcaactttaa agtaataaaa ataaacacct gtaccatcaa acttttcaat cttaccacaa
     2701 aagcactttc caagtttatc attatctaat tcttcattta aagttattgt tctcatttct
     2761 tcttctctat ttatttctag ggaaatagac ttgatattgt atccctcatt tactttaaca
     2821 caaatggtca attcctcatc aaattttaaa gctccaaacg gagccttaaa atttttatcc
     2881 caagagttat attctattac actttgcatt taatcacacc cttttaaacc aaatgtcttt
     2941 tgcatactca gaaattgttc tatcagaaga aaaaaatcca gagttagcta tattaactaa
     3001 agacatttta ttccattttt ctttatctct atataacctg tttatatctg cttgagcttg
     3061 accataattt tcaaaatctc tcaatacaaa atactcatca ttgtaagtag tgagtgaatt
     3121 ataaatactt cttccttctt cgccaagatt aggaataaat ccatttatta aatcatctac
     3181 tacccttttt atatccatat tagaatgata taggtcaagt gaagaatatc caccatattt
     3241 attataattt agtacttgtt ctgcactaag tccaaataaa aacatatttt ctttaccaac
     3301 ctgttcacat atctctacat ttgcaccgtc aagtgtagcc aaagttatag caccattcat
     3361 cataaacttc atattacttg ttccagatgc ttccttagtt gtagttgaaa tttgctcact
     3421 aacatttgct gctggtatta ttatttctgc taatgatact ccataattct ctaaaaatac
     3481 tacttttaac ttgtccttta ctctaacatc attatttata gtacttgcaa cagaatttat
     3541 aaatttaatt atacatttag ccaaataata tcctggtgct gcttttgcgc caaatataaa
     3601 agttcttggg tcaagattca aattaggatt atcaagtaat tcatgataca tatgaagtac
     3661 attaaatata ttcagtaact gtcttttata cgcatgcaat ctcttaactt gaacatcaaa
     3721 tatcgaactt gggtctacat ttaaatcgta tttatctttt ataaactttg ctagatttgc
     3781 tttattattg tattttatat catcaagtct ttgtaaaaca gaggaatcat ttttaaattt
     3841 ttcaatattt tttaattgta atgtatcttt cttccatgaa tcaccaatta aatctgtaat
     3901 taaattgcta agttgaggat ttgaacttat aagccatcgc ctatgtgcaa ttccattagt
     3961 cttattatta aatttatttg gttcatcttg atagaaatct tttaatacct ctgtttcaag
     4021 aatatgagta tgaagcttag caactccatt tacactatga cttgtgacaa tacataagtt
     4081 tgccatattg atattgtcac agtctattat actcattctt tttattctat cttggtcata
     4141 tcctttatta tttaattcct ctacatatct tctatttatt tcttctatta tcatataaat
     4201 tcttggtaat agttctttca tcatacttac aggccatttt tccattgctt ctgacattat
     4261 agtatgatta gtataggaca ctgttttttt agttatttcc catgcttcat cccaagataa
     4321 attttcttca tcaagcaaaa ttctcataag ttctggtatg cataaggtag gatgtgtatc
     4381 atttatatga attgctacct tatcgaataa attattaata tttaatttgt tctttttata
     4441 ttttcttatt atatcttgta tccctgcact tacaaaaaag tattcttgtt tcaacctcaa
     4501 caacttacct gcatagtttg aatcatcagg atataatacc tgtgatattt cctcagtata
     4561 atacttatgt tttaaagcct cttcatagct accagaataa tttagagcat ttgaggtaag
     4621 ttcaccaaaa tctctctttg gtatttcact cttaaacaat cttaaagtat ttatacattg
     4681 gttttggtat ccgattattg gtatatcata aggcattgcc attacaggaa ggtagttttt
     4741 atgaattact tttaaatgac taccttcctt gattaattct acctcaccac caaacttcac
     4801 cattgttgct tcatttggtc ttacagtttc ccatgcatat cgtccttctg taagccagtt
     4861 atcaggcact tctacctgat aaccatttac aaatttttgt tcaaacagac catatttata
     4921 ccttattcca tatccttgac cacttatgtt caaagatgcc atagaatcta aaaagcaagc
     4981 tgctaatcta ccaagtccac catttcctaa ggctgggtct acttcagctt caattaaatc
     5041 atccaagttg attcctaatt cagataaacc ttctctaact tcttcttcaa tatttaaatt
     5101 taataaatta gattttaact gtctccctat taaaaattct aaactaaaat aataaacttc
     5161 ttttttctta tctaactttg tagcaaccca tttttttgct atctcatctt tcaatagact
     5221 acataataca tttaacaatt gttcatcagt agcttccttg atagattgtg catataagct
     5281 atacattttt acttcaaatt tttgcttgaa tttctttttg cttattgtta ccaaaaaaac
     5341 cactccttta atcacatatg tctctatatg tttctatata ctccttagct gattttttcc
     5401 aactattatc tgttttcatt gcattttcaa ctaatttaat ccatttttct ttgtccttaa
     5461 atactttgat tgcatttttc aaacagaaaa acatctcatg agcattgtaa tttttaaaac
     5521 taaatccatt gccttctcca gtaaatttat tataaggtgt tactgtatct ctaagccctc
     5581 cagtctctct aactattggt aaagaaccat atctcatagc taacatttga cctataccac
     5641 aaggctcaaa taatgatggc attaagaaca tatcacttgc agcatatatc tgttgagcca
     5701 atgatgcatt aaaagtaatt ctagctgata ctttatctga aaaattagaa tcgtaatagt
     5761 tgaacattga ttgatattga tgttctccag ttcctaaaac gacaatctgt aaatcttctc
     5821 tcataagttc tggcatcatg tatgctatta aatcaaatcc tttttgagaa actaatctcg
     5881 aaactatacc tatcaacgga atagatgaat ctttttttag tcccaatata tcttgtaatc
     5941 tcaatttatt ttctactttt ttgtcaattg agtctacatc ataatgaaca aatatatctt
     6001 tatcttttgc agggtcattt aaatcataat ctataccatt taatatacct tttaattttc
     6061 ctgactcttt tctaagtaat ccatctaatt gttcgccata aaaagatgtt tgtatttcat
     6121 ttgcataagt agggctaaca gttataattt tatctgcaaa atttatacca gctttcataa
     6181 aatttattga gccataatac tctatatctc cattatcaaa gtatttttgt ggtaaatcca
     6241 atatatcata taaaacatgc ttatcaaata caccttgata ttggagatta tgtatagtat
     6301 acatagtttt tatattttta tacccctcaa gggttgcata tctttctttt agaattaatg
     6361 gtaacatccc tgtatgccaa tcatttatat gtattacatc tggataaaaa tcaattctac
     6421 ttatagcttc taaaactgca tctgtaaaga aagtatatct ttctgcatca tctccatatc
     6481 catagatact ctttctttca tcttctcttc taaaataaaa ttcattatct ataaagtaaa
     6541 attttacacc atctaattcc atttctaaaa gaccacagta ttgatttctc catccaacac
     6601 gtacactaaa cacagctatt tcttttagtt gatcttttaa ataacttggt atagttgaat
     6661 atttgggcat tattaccctt acatctacac cttctttttt taattcttta ggcaatgcat
     6721 aagaaacatc tcctaatcct ccagtttttg caaagggcca acactctgct gttacataaa
     6781 aaaccttcat tagtcattcc cccttctaaa aaatttattt atatatttgt attcttttcc
     6841 actacaagag gatatttttc atctcctctt aattctttcc catttgatac actcgtattt
     6901 ttatctaata ttgcattata tagttttgca tttgacttta ttacacaatt ttgcattact
     6961 attgagttct ctataactgt tcctttttca acatttacct ttctaaatat gatactatct
     7021 tttacagtac cttctattaa acaaccagat gctataaagc tattttctac tttagcacta
     7081 tctgtataaa ttgtaggtga ttcattcttt tcttttgtaa atatctttct ttcagaatat
     7141 aataattcat ctgcaacttc taaatttaat aaatctttgc tcatttcaaa atatgattca
     7201 acagaattta cacattttag atatcctgta tattcatagg ctcctatttt catgttttct
     7261 gcttcttcaa taataaattc ttctaaataa caataattgc ctttatttac aatgttatat
     7321 atacattcaa caaaatcttc acgcttcata atatatgtct ccatacatat gtttgctcta
     7381 gggaacttac ctaaattatt tcctacattt attaccctat tgttactatc taaatttaga
     7441 gttaagttac cagcaaaatc tttatttgca ttatttacat ttttatatat tatagttata
     7501 tcattatgag attttttatg gtattccaat gcttgagaat aatttaaatt gcatagcata
     7561 taacttggag ctattaaaat atattcttct tcactctttt ctatataatc tagattagct
     7621 aagatagtat atatatcacc ttctctatgt gagtaagtat ttctatactt agtgttttct
     7681 ggactaaaaa ttgataggcc accttttttt ctacttaaat cccaatcttt accactacct
     7741 atatggtctg ttaatgatct gtatttttct tttgcgaata ttccaacttt agtgattcct
     7801 gaatttatca tattagataa agcaaagtct attattctat atcttccagc tattggtgta
     7861 gatgctattg gtcttccata gttaagtttg tttattgctg ggtctccttt tttgtttaaa
     7921 tttattatcc ctaaacattc atttctcatc ttaaagacct ccttctatta atacgcatct
     7981 aggttcgata ttttcatact cagaaactac atttatttca ttatttacat tctttatctg
     8041 aacattatca tttatagttg cacactcacc tatcatagct ttttctatag ttacattttc
     8101 acctattaca acatttggca ttattactga atcaattact ttacttcctt taccaacaac
     8161 aactccatgt gataatacag agttacctac ttcacctaaa attctacaac catcagctat
     8221 cattgatcta tgaacatttg catttttacc tatatattgt ggaggcattg ccatagtatt
     8281 tgtatatatc ttccacttag ggtctgctaa atcaagtgtt tctggagatt ttattatatc
     8341 catattagca tcccatagac tttgtatagt tcctacatct ctccagtatc ctttaaatgg
     8401 ataagcatac attccaacat tatcttctaa cattttaggt attaaatttt ttccaaaatc
     8461 atcataattt atttctggat ttttttctgc ttccttaaaa taatttctca acattttcca
     8521 atcaaatatg tatactccca tagatgccaa attgctctta ggttcacttg gtttttcttc
     8581 aaattcatag atacttgaat cttcatttgt attcattatc ccaaatcttg aagcttcatc
     8641 ccaaggaact tcaatcactg ctattgtagc tttagagcct ttttccttat gaaattttaa
     8701 cattttgcta taatccatct tgtatatatg gtcaccagat aatatcaata catattctgg
     8761 attataggta tctacaaagt ccatattttg atatatcgca tgtgctgtac cattatacca
     8821 atttccttct ttttcattca taaatggttg taaaacatag acaccaccat ttattctgtc
     8881 caaatcccaa tgacttccca tgcctatatg tgaatttaat atcaacggtc tatattgagt
     8941 taaaacacct acagtatcaa taccagagtt tgaacaattg cttaacacaa aatctataat
     9001 tctatactta cctccaaatg atacagctgg tttggcaatt ctttttgtaa aaacacctaa
     9061 tcttgaacct tgccctcctg ccaaaatcat agctaacatc tcttttttca tttaatgaca
     9121 ctcccctcat ctagtaatta atatttataa ttgcattttt acttttataa actttatcag
     9181 tcaataaatg tcgtgtataa atgttgctta aataatacct taataaaaat aaaataatat
     9241 caactataca attgatataa tgtatacccc ataatggact taccgacgta tacattattt
     9301 tccataagca ctatagtatt tattaatatt caattatcgg ttaaaaaata atacaaattt
     9361 tattttataa atataaaaac attttttata aatcccttgt gtcatataaa catgcttatt
     9421 ttttaatttg ttaaattcgt aatagcaaaa tataatctaa tatttgttaa ataattatcc
     9481 aaagaaaatc aagtaattat taatcaactt tattttttaa ttgatacttc tttgtctata
     9541 atttaaacaa aaggatataa acttttaaat tatttattag ttttcttagt attaatgttt
     9601 atatttttat aaatatatat taataatata aatagactaa ctaaaaccat gcttatttgc
     9661 tatatgtttt atataatatt atataacact ttttacatta gtaaaggata aattgtataa
     9721 agattctgtt aatttatttt tataatttca tctttctaat aaaaacaaag aagaagactt
     9781 aaaaatttat aagtcctctt cttttcaacg tgcatttttg tgctatattt aattaattcg
     9841 acctttaatt tccatatcct tcctatgata acaaaactct tttatttttt taatgattca
     9901 ttatcattta aaacttcttt tatatcaagt acttctttgt tatcatcact cataacttcc
     9961 tttattcctt tgaatgttcc taggaaatta actgcttctg aaggtaaaac cagttttgtt
    10021 gacttacctt cagccatttt ttcaagagct tccatagatt ttaatgctag aatattattg
    10081 tctatatcag cctctttcat tgctgaaaaa actaatttta aaccttctgc tgttgccatt
    10141 tgagttcttt ttatcatttc agcttcacct tgtgccttag caatagctgt ctgtcttata
    10201 gcttcagctt caccttctgc cactaatata gcagattctt tttcaccttc tgctacacgt
    10261 accatagctt cttttttggc ttctgctgtt aatattgcag attgtttttc accttctgct
    10321 tgaagtattg cagctgactt attaccttct gcttgaagta ttgcctctct tctttctctt
    10381 tctgctctca tttgtttttc cattgcaact tgaatatcct gaggaggcat tatatttttt
    10441 aactctactc tatttacttt tataccccat ttatctgttg cttcatcaag gatagttctc
    10501 atttttacat ttattatatc tcttgatgtt aatgtttcat ctaaatcaag ttcaccaatt
    10561 atatttctta gtgttgtagc tgttaaattt tcaatagcag ctattggatt agctatctca
    10621 aacacatatc taactgggtc tgtaacttta taatatacaa ctgtatcaat ctgcatagtt
    10681 acattatctt tagttataac tggttgaggt ggaaaatcta ttacaatttc tctaaggtca
    10741 attacatatg ccattttatc taaaaatggt attaagaaat gtacacctgt ttcagccact
    10801 ttttgaaact taccaagtct cattattata cctactttag attgttttat aactcttata
    10861 caagttaaac ttattgctac gactactact attaatacaa tacttaaaac aattttagta
    10921 cccatactat aaatcctcct ttattctatt ggtttgtcac attaactgtg gcgtctgctt
    10981 ttttaacaac taattttacc ccttcaattt ttaatacttc tactgtagaa cctttttcta
    11041 tcttttcacc atctatagaa atagccgtcc atgtttcatg ttcaactaca actagacctg
    11101 tgttatttgg agaaatctct tctgtaacaa tacctttttt agacatcatt gcttgtaaat
    11161 tagtgttaga tttatagcct ttatcttttt ttactatttt tttagtatct acgactaaca
    11221 ttgctataga aattactgca aatattaaaa tttgtaagaa aatactctct ataaagctac
    11281 ttaaaaatat aagtattaca gctcctacac taaaccatat caatgttaag cttggtgtta
    11341 acatttctgc tattccaaat aagactgcta ctatcaacca tattaacttc atactatcta
    11401 ccctcttttc atatgaatta taaaacccta tataaattat atgtttatac aatgtttttg
    11461 aatataaatt taaattacaa aacaataaaa taaattaatt aattaatagt attatatcaa
    11521 catactttgt taaaattgtt acaaagtagt atcaaatatc accaattata tatccaacaa
    11581 ctctttcatt gttaatgaaa tgttacttat taaatccgta gcatttaccg tatacattgc
    11641 tttgctcaat ttatctccta tatagccatg tatatatgct ccactaactg tagcttccaa
    11701 tatatccata ccttgaccta taaatgaagt aatcattcca agcaaacaat cacccattcc
    11761 accagttgcc atacagctat ttcctgtagg atttatatat gtttttttac catctgttat
    11821 gacagtttga taacctttta aaagcaccac cagattatat ttttgagcaa attcttttgc
    11881 aacatctact ctatgttctc ttatataccc aattgaatct cctgtcaacc tggccatttc
    11941 tcccaaatga ggtgtaatta caaatctatt tttccattct aaaaacttat aacatctgtc
    12001 cttcataacg ttcaatccat ctgcatctaa tactattggg caattagaat tttctactat
    12061 cttaagtaat ttatcaaatg tttgactatt atctcccata cctggaccaa aaccaattgc
    12121 atcactagaa ttaagtaatt tatttaatct ttcttcttca aaatttaaag tcattgcttc
    12181 tgtaagtcta atagatacct tttcttgcac atcttcacta ctaattaatg ttacaagtcc
    12241 actcccactc ttaacagcag attcagttgc tatgaaactt gcaccataaa atccttttga
    12301 acctgcaact atagatactt ttccaaaatc acttttaaaa ccatgcttat ctcttcttct
    12361 aatattattt tttacaaaag tcttatcagt tatgaactct tcatcatggt attttcttgt
    12421 aataaaatct ggaacaccta tcttttctac aactacatca cctataagtg tgctagtgtc
    12481 atagtttaga aaacctcttt tataaaactc aaagctaatc gttttgtttg ctctaataca
    12541 agttcctaat acatttccat tatcgctatc tatacctgaa ggaatatcta tagaatatgt
    12601 atttttgcta ttatcattta ctatgtcaat aacttcttta aaaataccag ttatatcacg
    12661 ttttaatcca gtcccaaata tagcatctac tacaacatca cttttagata ctctatcttt
    12721 aaaaattgtt aaatttttat tttttataat attttgtttc tcaactttaa taattttatc
    12781 tacttcattt tcatctccat aatttttttc acttaaatca tttttattaa tatttataat
    12841 attaattccc atatttaaaa gtacattgta atttatttta gagcattcac ttagtttttc
    12901 aagattacct actaaaaata cgtttacttc ttttccatat gtatttagat gtcttgctat
    12961 tccaagacca tctcctccat tatttccaac accacataca actgtataac tattatacat
    13021 gtctatatcc atatttttca ttgcagatat aaccgcattt tccatcaata caatcattgg
    13081 aatccctact tcttctacac atgcagtatc aacatttctg cttaatttag atgtacctat
    13141 tagcataaat tatcactcct ataaagttaa agacttctta aatacttac
//
LOCUS       DAELJO010000105        13128 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELJO010000105 DAELJO010000000
VERSION     DAELJO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.105"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            157..1218
                     /locus_tag="KPB88_002426"
     CDS             157..1218
                     /locus_tag="KPB88_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0054695.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1231..1428
                     /locus_tag="KPB88_002427"
     CDS             1231..1428
                     /locus_tag="KPB88_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0054696.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1592..2296)
                     /locus_tag="KPB88_002428"
     CDS             complement(1592..2296)
                     /locus_tag="KPB88_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0054697.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2461..3186
                     /locus_tag="KPB88_002429"
     CDS             2461..3186
                     /locus_tag="KPB88_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0054698.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3246..4838
                     /gene="hcp"
                     /locus_tag="KPB88_002430"
                     /gene_synonym="priS"
     CDS             3246..4838
                     /gene="hcp"
                     /locus_tag="KPB88_002430"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG0054699.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5210..6115
                     /locus_tag="KPB88_002431"
     CDS             5210..6115
                     /locus_tag="KPB88_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0054700.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6239..7192
                     /gene="msrA"
                     /locus_tag="KPB88_002432"
     CDS             6239..7192
                     /gene="msrA"
                     /locus_tag="KPB88_002432"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG0054701.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYNGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7345..8475
                     /locus_tag="KPB88_002433"
     CDS             7345..8475
                     /locus_tag="KPB88_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054702.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8592..9560)
                     /locus_tag="KPB88_002434"
     CDS             complement(8592..9560)
                     /locus_tag="KPB88_002434"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG0054703.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            9895..10083
                     /locus_tag="KPB88_002435"
     CDS             9895..10083
                     /locus_tag="KPB88_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054704.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCEKGQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            10343..11542
                     /locus_tag="KPB88_002436"
     CDS             10343..11542
                     /locus_tag="KPB88_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG0054705.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11594..12097
                     /locus_tag="KPB88_002437"
     CDS             11594..12097
                     /locus_tag="KPB88_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0054706.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12204..12521
                     /locus_tag="KPB88_002438"
     CDS             12204..12521
                     /locus_tag="KPB88_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054707.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12679..>13128
                     /locus_tag="KPB88_002439"
     CDS             12679..>13128
                     /locus_tag="KPB88_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0054708.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFP"
BASE COUNT         5095 a         1392 c         2249 g         4392 t
ORIGIN      
        1 caaaaaaact atagaaattg acatatattc cagtatttgc taaagtagct gttggggaaa
       61 attacaaaaa ttattaatgc gcattagata atttacttta atttttttag catttggggt
      121 aatttataat ataaagcatt agaaaggaaa atataaatga gggacttttt aaaaaattat
      181 aagtcatcaa ttatactttt agcatgtact atagtagggg cgatagtagg attagtactt
      241 ggtccaaaag cttcaatact aaaaccattt ggagatttat tcataaatgc tctattaatg
      301 atagtagtac cattagtttt tcttagtgtt tcttcaggtg ttgctagtat aaaaaatatg
      361 gaacgatttg gaaaaataat gaagggtaca gctatagtat ttatgggaac aacacttgta
      421 attgcatttt taggtattac atctgcatta ttatttaatc cgtctaaagg attagatgta
      481 tcaagtgtaa aaaatttaat ggtggctcaa gatgcagaaa ctttagaatc aaaaggaacg
      541 ttattagacc aagtagtatc aagtattaca gttaaaaatt ttggagattt attttccagt
      601 agtaatttac tacaattagt tatatttgca gttttaattg gaacagcaat agctatgatg
      661 ggagataggg gaaatacaat aagaaagctt ttatcagaag gaacggaagc aatattaaaa
      721 gtagtaagta tattcatgct ttatgctcct atagggctag gctgttatac agctacaata
      781 attggggaac ttggaagtaa aataattcaa ggatatgtaa gggtgtttgt aatttattca
      841 ctaacagcag ccatgtattt cttattattt tacagtctgt atgctttttt atctgcagga
      901 aaacaagggt ttagacaatt ctggagaaat gctataaaac caatagcaac ggcactagct
      961 acttcttcaa gttcagcatg tataccatca aatttagaag ctagtaaaaa tataggagtt
     1021 cctgatgata tatcagaaac agttatacct cttggaataa atatacataa agatgggtca
     1081 gttttagcag taataataaa agtggcattt ttatcaggta ttttaggtag tggaatggca
     1141 aatccagcaa cgataggtca attatttata actgtatttg ttgttggagt atttatggct
     1201 cctgtagcag gtggctgact tattggtgag atgctcgtgg ttagcatctt cggttttcca
     1261 gtggaggcaa ttccactgtt aacagttata actactataa ctgatattcc taatactata
     1321 ttaaatacaa ctggaaatac agtatcatca atgttagttt caagattagt agaaggaaaa
     1381 gattggctta tagataaaac agcaattact acaaagaaaa taagctaatt gtacacttaa
     1441 ttaataaagt taataggtat cttaatttta ataaattaga tacctattaa ctttttaagt
     1501 aaaaatataa atttaagatt caaatatgat atttataatt tgaatcaact aatatagtat
     1561 atattgaaat aaatatgttt aaatatctat tttatttttt aaaataaaag atttcaaatt
     1621 tatttttgtt aaaagataac aagccttcat ctcgcatctt gccaagctct cttgacatag
     1681 aacttctgtc aacacataaa aaatcagcca tttcatttct tgaaaaggga attgtaaact
     1741 ggtggctacc tgtttgttct atctgttttg aaaaatacat aagcaatttc tcgcgagttg
     1801 ttttacgaga aagtaattct attttattgt gaagtaaaat gtttttttca gctattaatt
     1861 gtagcatatt ttcaacaagc ctattatgaa atacacatgt agatgaacaa ataccagtta
     1921 cacttttaaa ctttataaat aaaacttcgc aagaagatgt tgtggttact gtaacattac
     1981 ttttatgtag tctagtacat gcgaatacct ctccaaataa atctccaggc aagagttcag
     2041 ctacaatatt cctattccct tcgatatctt cctttattat atgagcactt cctttaagca
     2101 taattccaat agaagtaact tcacttccaa cagagaatat tatactatct tttgggtaag
     2161 ttttatctac aacacctaaa cattccaaca tttcttcaag ttcatcactt ttaattcctt
     2221 caaatagagg aattttttta agtaggtcta tatgcttttc taaaaatttt ttcaaataat
     2281 cacttccttg ttgcatatgc aaccgataat ttgttttcat tgtactatac taagtttata
     2341 aaagcaaggg gatataaaaa tcctactaca agaaatttga aataaagaaa tataggtatt
     2401 aaaaataaat atataagaaa tcaatacttt aaattaaaaa aattttatga ggtgatataa
     2461 atgatgagaa aaataattaa aattgatgag gaaaagtgta atggttgtgg actatgtgtt
     2521 gaagcttgtc atgaagaagc tataggaatg gtaaatggaa aagcaaaact tttaagagat
     2581 gactattgtg atgggctggg agattgtctg ccaacttgtc caacaaatgc gatttcattt
     2641 gagtatagag aagcagcaga atatgatgaa gctgcagtaa aagcaaatat ggaagcaaag
     2701 aaagcacaaa agaaaacttt agcatgtgga tgtccaggtt ctcagtctaa gtcaataaat
     2761 agagaggttt caaatagtac gtctataagc aatgatatag tagagataaa aggttctcaa
     2821 cttaatcaat ggccagtaca aataaaatta gtaccaacta atgcatcata tttaaagaat
     2881 gcaagtcttt taatagcagc agactgtaca gcgtatgcat atggaaattt tcataataaa
     2941 tttatgaaaa ataaggttac attaatcgga tgccctaaac ttgatgaagt tgactatgct
     3001 gaaaaactta cagagatttt aaaagaaaat gatattaaaa atatagtagt aacacgtatg
     3061 gaagttcctt gctgtggtgg tatagtaaat gctgttaaga cagctctaca aaatagtgga
     3121 aaaatgatac catggcaaat agttactata tctgtagatg gaaaaattgt agatggagaa
     3181 atttaattaa gtgaattata gaaaaatata ataaatttta ataaataaaa agggaggaat
     3241 aaaaaatgga aaaaatgttt tgttttcaat gtgaacaaac agcaggaggt aaaggttgta
     3301 ctataaatgg tgtctgtggt aaaaaggggg gtacagctaa cttacaagac gaacttacag
     3361 gagtgttgat tggtctttca agagcaacta ttggaaataa gaatagacca acaagtgaaa
     3421 cagataaaat tatgatagag gggctattca ctactataac gaatgtaagt tttgatgatg
     3481 aagctataaa aagacaaatt gaaaaaactg aggtagaaaa aagtaaactt gtacctagat
     3541 gttcagattg ttcaacaact tgtaatagaa atgataacta tgatatgaag gatttatgga
     3601 atgataatga agacatacgt tcattaaaat cattaatctt atttggatta agaggtatgg
     3661 ctgcctatgc atatcatgca atggtacttg gttatacaga caaagacgta aatgaatttt
     3721 tctatgaagg attaatagca gttggaggag acttgtctat tgacgaactt ttaggtctag
     3781 ttatgaaaac tggagaaata aacttaaaat gtatggaatt gcttgataga gcaaatactg
     3841 aaacttatgg aacaccagaa cctacacagg tatctatgaa aatcgagaaa gggccattta
     3901 ttgtagttac aggacatgac ctttatgatt tacacaaatt attagaacaa acgaaagata
     3961 agggtataaa tatatatact catggtgagt tattaccagc acatgcatat ccaaaactaa
     4021 aagaatataa acatctaaaa ggtaattttg gtacagcatg gcaaaaccag caaaaagaat
     4081 ttgataatat tcctgctcca atattattta caacaaactg tttaatgcct gttaaagata
     4141 gttatagaga tagagtattt actacagaag tagttgctta tccaggtatg gttcatattg
     4201 atgaagataa agatttttct agtgtaatag aaaaagctct tgaacttgga ggatatagag
     4261 aagatgttca tatgactggt attaatggag gagatacatt aactactggt tttgcaagag
     4321 gtacagtttc atctgtacaa gataaagtag tagatgctgt aaagagtgga gctataagac
     4381 actttttatt ggttggaggt tgtgatggtg caaaacctgg aagaaattat tacactgaat
     4441 ttgtaaaaca agctcctaaa gacactgtta tattgacact tgcatgtgga aaatatagat
     4501 ttaatgattt agaccttggg gatataggtg gcttacctcg tttaatggat atgggacaat
     4561 gtaatgatgc ttatagtgca attaaagtgg cagtttcatt ggcaaatgct tttgaatgtg
     4621 gagtaaatga tttgcctctt agcatggtac tgtcatggta tgagcagaaa gctgtttgta
     4681 tacttcttac actacttcat ttaggtatca aaaatatcta tataggacct tctttaccag
     4741 cattcttatc taagaatgta ttagatatat tggttgataa atttgcattg acacctatat
     4801 ctactcctga agaagatatg aagaaaattt taggataaat aaatatatct atacttaaag
     4861 atatttatag ttaaactatt tttaagtatg ccacaattaa gataaggggt taaacttaaa
     4921 atttgattaa aaaatcaatt ttgagatgac cctttattta ttttttgtaa taattgaaaa
     4981 ataaacctgc tttcagtata ttttttttaa gaatctttgt attaaattta taatacttag
     5041 atatataata tcttatatat tttattttaa gtattaaagg tttgaatgta acttaaatat
     5101 taaaagagga acattgtaat atgtaacttg cataaaatat atatactata gtgaaattat
     5161 tttgattaaa atttaaaata taaggtgttt tatgtggggg tatatgtata tgaaaaattc
     5221 taattttaat atattggcat ttgatggtgg tggattaaaa ggggcattga gtatatccat
     5281 acttgagagg atagtaaaag agtatccaaa tctattaaac aatataaata tgtttggagg
     5341 tacatctaca gggagtctaa ttgcactagg gcttgcatat ggtgtaagcc ctaaagagat
     5401 aaaagaactt tactcaattg aaaattcaaa gtatattttt aataaaagtt atgcagaaat
     5461 actcagacca aagtatgaaa acaaaaactt aaaagaagtt ttattaagta tatttccaga
     5521 ggaactagaa ttaaaagatt taaataaatt agtaatgata ccaagttttt atataggaaa
     5581 tgaggaaaat gcatggaaac cagtatttta taacaatatg ccaaattctt ttacaaagac
     5641 tagcaaagtt gtagatgtag caatggcaag tagtgctgct ccggtatttt ttccaacata
     5701 taatcgtcat gttgatggag gaataatagc aactgacccg agtttagcgt gtataattca
     5761 tgccattgac agtggattta gacttaagaa tacaagactt ttttcaatag ggactggata
     5821 tgtatataac agtataaagg cagatactac cgaatgggga gctattgact ggataattaa
     5881 taaagaaccc gatttaccaa ttatatctat taccttagaa ggaaattctc aaatgagtca
     5941 gttattttct caaaaacttc ttggagataa ttactataga ttaaatccta agatggaaaa
     6001 agatgtagct atggatgatt gtgatgcatt agaatatcta atgtctttag gagaaaattg
     6061 tgatataaaa gatgcttttg gatggataaa gaaaaagagc ttttatagaa attagattca
     6121 atttgcaatt gtatttttac acatgatttc attgagtagg taaaaataat aaaatgcaga
     6181 ttattaagtt acttcttagg gaataaataa agtataattt atttaaggag tgatgaaaat
     6241 gataaaactt gctacttttg caggtggatg tttttggtgt atggtaaaac cttttgataa
     6301 gtataatgga gttattaaag tagtttctgg atatacaggt ggtcatgttg aaaaccctac
     6361 atatcaagag gtatgttcag gaactactgg tcatttagaa gcaattcaaa taacatatga
     6421 tgatgatata ataagttatg atgaacttct aaatatcttc tggaaacaga ttaatcctac
     6481 agatgatggt ggacaatttg gtgatagggg aactcaatat aagactgcta tattttatca
     6541 tgatgaagaa caaaaaaata tagctttaga gtcaagagaa catctggaaa atagtaaaat
     6601 atttgatagt aaaatagtga ctgatataat tccagcaact aaattctacg aagcagaaga
     6661 aaatcatcag gattattata agaaaagacc attaaactac aatatgtatt ataaagggtc
     6721 cggaagatat aattttgtaa aaagtcattg ggatggaaac aatataaata gggaagagtt
     6781 aaaaaagact ttaactccta tacaatttga agttactcaa aatgatatga cagaagtacc
     6841 ttttgaaaat gaatattatg acaataacaa agagggaatt tatgttgata ttgtaagtgg
     6901 agaagtgtta ttttcttcaa aagataaatt tgattctgga tgtgggtggc caagtttttc
     6961 aaagccagtt atagataaat ctatcattga gagaagtgat ttctcacaag gtatgtacag
     7021 agttgaagta agaagtgcta aaggaaattc tcatttaggt catgtatttg atgatggtcc
     7081 aaaagaactt ggaggtttaa gatattgtat aaattctgct tctcttaaat ttataccaag
     7141 agaaaatatg gaagctttag gatatggaga atatttaaaa ctttttgagt aattaggaat
     7201 atttattata aacttatcta aaaggtgaaa atctaaaaaa atattttcac cttttgtttt
     7261 tttgtcaata gtatgataaa ttattaattg taatcttatt tataccaata gattaagaat
     7321 ttaattttga aagagagaaa acaaatgaat ttaaaagact ttaatggaaa gagattaaaa
     7381 tcagctagaa tttttagagc aaagacgata gaacagttgt ctaaagaaac taaaataaat
     7441 aaaaaagatt taaaagcttt tgaggaaaat aagtatgtac caaatataga aaatacatta
     7501 aaattatcta atatattaaa ttttccaaaa gagtattttt ataaaaatga aaatataaat
     7561 atcgttgtag aggatagcca ctttaaccct caatctaaac tacctagagt agaagagatt
     7621 tcttatagag aaaaaataat tataatacat aaaatatatt catttatgga gaattatatt
     7681 aagtttccaa aagaaaactt gccaaataga aaagtaatgt cagatattaa tataaatgat
     7741 atagaatctc ttgcatataa gactagagag ttttgggatt taaatagtac tccaatagta
     7801 aatatggtaa gtttgttaga gtctaaaggg attataatat caggtatgaa tgtagataga
     7861 aaaggggcaa ctatctttac tcaaaaacag agaattagta aagaatctaa atatctaata
     7921 tctctgggta atgacaaaaa gtcagcatca attagaaatt atactttggc atgtgagctt
     7981 ggatatataa tatcaagtga attaagaata ccaagtaagc aattttcaga agatgagtat
     8041 gcctgtgcat ttttattgcc aaaagagagt tttttaaaag atttgactca tccagaagat
     8101 ttagattatt atgtagaact aaagaaaaaa tggattgtac ctatatctgc tatgattttt
     8161 agagcatata atctagaaaa gataaattac aaaaaataca attatcttat gaatgaaatg
     8221 gataaaaaag gctggttgat agaagaacca ctagacaaaa tgaaaggttc aagcccaacg
     8281 tatttaaaaa gagcagttga attattgata gaaaataaaa taatgagtgt aaatagtata
     8341 gtatcaagtt tagaggaatt tcgaataaat ctttatccag aagacttaga attattgatg
     8401 ggattgaaaa aaggattgtt atctcaagag gtaaataaaa aaagtaaagt tataaaattt
     8461 gatggtaaaa aataggttta tattttagta ttaaaattaa aagcaggtac aaatgattta
     8521 aacattaact tagttaaaag tcttttcaat aagtcattta cctgctttta gtgtaagaaa
     8581 gaatattgaa tctaaagtgt ttttgaaagt gcaatatatt cccttaaaac agaacaagat
     8641 ttattaaatt cgttttgctc atattcagtc atattgattt caataatttc ttcaatacca
     8701 tcttttccta taacacatgg aacacctgca aatacatttc tttcgttata ttgaccttct
     8761 agtaatgtag aaactggaag aacttttttc tcattgtgta gtatagcttt tacaatttct
     8821 actaaagcag tacctatacc aaattctgta caatctttac catttattat acttatccct
     8881 gtttttttat ttttttcaac tattgatggt aagtctaaat tgctataagt acttgggttg
     8941 tcttttatca tgtctaagat tggtttacca ccaattgaaa catgagacca aggaaccatt
     9001 tgagaatctc catgttcacc catagaataa gcttgaattg attgttgagc gattccagtt
     9061 tcttcagata aaatccttct aagtcttgtt gaatctaaag ctgttccagt tccaatgact
     9121 ttgtttttag gaaagccaga tttttcccaa atataatttg taactacatc aacaggatta
     9181 gatatgacta caaatatacc atcaaagcca gaatcaacta tgggttttac tatagatttt
     9241 atcattccaa tagttggtct tagtaaatct aatctagttg tgttttgtct acgtaaaggc
     9301 ccttctgaag agtctgcaac acttatcact actatgtcag aatctttaca gtcagaaaaa
     9361 gttccttttt ctatatgtac tttatgtggc agatatgaaa ctgcatcagc taaatctaaa
     9421 gcttgagctt ttgatttaga ttcatctata tcaatcataa agagttcatc acaaactcct
     9481 tgagttatta aactaaatcc acaatgagaa ccaacatgcc ctgaacctac aatagatatt
     9541 tttcttggct ttattgccat tttaaatcac tccccctaaa ataacgatac attgaaaatt
     9601 atagatgtta ttaaaagctg tttttggata ttttaatatt ctattatttc agaaaattta
     9661 tttaatatga ttttatacta aaatttttaa aaaagcaatg ttaatatact ctctattagt
     9721 gaatattaaa gtattatatg tatattaata attattaaaa tatgtaatgt cactgatagt
     9781 tagaatattt aagtgttaaa agatgtgtat aaataataat gatgtcagtt tataatgata
     9841 ttaaattgag gtatactgtt tctctaaatt aaaaaataaa ggagtaaatg tgttatgaat
     9901 tatcatacaa aagaagaatt ggtggaagca ctgagagtag tatcttcaag tattattaat
     9961 tgtgaaaaag gacagaagaa attttctgag gagacatctc atcatacacg ttttaagaat
    10021 attatagaag caatgtatat ttcaaaatca ttaataatgg atgaaattag taaaagagat
    10081 tgaagcttat tttgcaattt aacatgtttt aatatacatt ttattatata agaaaataag
    10141 taaacataca tgttataaaa tattggaata atatacaatt ataaatttgt taacactaat
    10201 atctatatgt taaaatatag tagaagatgt tagtaaataa taggatgagt tctattttaa
    10261 tacaattaaa ttaagcttag tttgaaattt gtatctaaaa agtaaagtaa tctagaatta
    10321 agaaaatgga gatagaatta taatggaaaa attatactat acaaatcagt atataagaga
    10381 ttttacagca gaaataattg aaataaaaga agtagaaaat aagtatcatg ttttattaga
    10441 taagacagct ttttttcctg gtggaggagg tcagtttgga gacctgggaa aaatagagaa
    10501 tgatgatgta ataaatgtgt atgaagatgg aaaaaaaata taccatgtat tagataaaaa
    10561 acctataaag atacataagg taaagtgtag tattgattgg gatagaagag aagatggaat
    10621 gcatcaacat tttggacaac atgtattgtc tggatgtttt tttaaaactt ttaatgcaaa
    10681 tacgacaggt tttcatttgg gaaatgagta tagtacagta gatatagaag gtaatttaga
    10741 agaggataaa attagagaga tagagttgtt tgccaatgaa attattcgtc aaaatataca
    10801 agtagagaca ttacttccaa cgaaaaaaga actaaaaaag atttggttaa gacgtgattt
    10861 gccaaatact gatgaagaga taagagtaat aaaaataggt gatttagata cgaatgcatg
    10921 ttgtggagtg catccaaaat caactggtga attgagaatg ataaaaataa agaaatggga
    10981 aaaacataag tcaagtacaa gaatagagtt tctagcaggt aaaagggcag tggatgaagt
    11041 attgaagagg gatatatatt tgactaaaat atgtagatat ttaagctgta gtgaggaaga
    11101 tgctataaat ggtattgtaa atctaaatag taaagtggaa gaggctttaa ttaaaaaaag
    11161 aaggcttgaa gaagtagtat ctacatatca ggtcaaagaa atgattgaaa aagcaaataa
    11221 ggttggcaat atttcggtca ttaaaaaaat ttatgatgat gaagatttga aatatgtaaa
    11281 taagatagca aataaaattg cagaatatga aaatagtatt gcattattag ctgttaaatt
    11341 taatgataaa ataaatttag tttttgcatg ttctaaaaat ttaaaagata ttaatatggg
    11401 agttttatta aaggatgcaa taagtcttat agatggcaag ggtggtggaa gtaaagtatt
    11461 agctcaagga ggaggtaaga ataatggaaa tttagaggct acacttgatt atgcatttat
    11521 gaagatagaa aaaactctat aaatatatta tataaaaaac cttagtagat aaaatagata
    11581 tggaggaata agtatggagc aacaaaagga ctacatagag ttatatgaaa atgaagaact
    11641 aatagagata agagaagcac gtatggatga tttggataca atagcaaaat ttaattataa
    11701 tttggctaaa gaaactgaag gaaaagaact tgatatggat gttttaacta aaggtgtaaa
    11761 agcattgtta ttagatgaga gaaaaggaaa atatcatgta tatacagtat ttgataaagt
    11821 tgttgcacag attatgtata catatgagtg gagtgattgg agaaatggaa atttcctttg
    11881 gattcagagt gtatatgtag ataaggaata tagaagaaaa ggtatcttta attatttatt
    11941 taattatata aaaaatatat gtgataaaga tgaaaatata gttggaatga gattatatgt
    12001 agaaaaagaa aacataaatg caaaagcaac atatgagtct ttaaatatgt atgagtgtga
    12061 ttataatatg tatgaatacg aagtaataca ttcgtaatat aaatttgtaa tactgttata
    12121 ctgatgttat aaagtattga ttatattgac gattatatga aataaactgg atattataat
    12181 tagaattttt agggggtata aaaatgggcg ttttaaaagg aatgttttat gtatttttat
    12241 ttttagactt agtgtcaata ttctgtttct ttttcaataa aggaaaaata gcatctaata
    12301 aaattgtgtt taatgcaata ggagtactta catttgtact ttgttttatg ctttttagtt
    12361 attatcctaa taataatctt attggaaaat ttattgcttc tctatttttt atatttggag
    12421 ttgcaggagt tatgttgaaa gaaaaaaatt ttttatatgc tagacttctt ttaactgtag
    12481 ttatagtttt ttctacatta agattgttcg ttattcaata aaatgtattt gtatgaaaat
    12541 ttattaaagt ctacacattt aagtgtaggc ttttttgatt ttttgtttga aaattaagga
    12601 aattttaagg aaattttaaa atcgtcttaa atatttattg atatacttaa aatattaata
    12661 gcgaaaggag acttatcagt gatagaaagt aaaaagtcag aaaaattgtt actgtcaatt
    12721 atatacataa tatttttggt atatatttta atgattataa gaataatttt atttagagat
    12781 gtaccaattt acgctatttt caagggaacg tttaggaatg taaatttaat tccattttat
    12841 acaatatatc aatttatagt agatagtaat cttgacttta tgaaagctac tgtaaatatt
    12901 atagaaaata taggtatatt tattcctatg ggaatttttt tacctatagt atgtaaaaat
    12961 ctaaataaaa aaactataat tatagtaatt atacttgtta gcctagcatt tgaattaaca
    13021 caatatattt ttgctcttgg aagtagtgat atagatgatg ttattttaaa ttcattaggt
    13081 ggcataattg gaattaatat ttacataaac atgaataaat tatttccc
//
LOCUS       DAELJO010000106        13055 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELJO010000106 DAELJO010000000
VERSION     DAELJO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.106"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            110..823
                     /gene="ispD"
                     /locus_tag="KPB88_002440"
     CDS             110..823
                     /gene="ispD"
                     /locus_tag="KPB88_002440"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG0054709.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            820..1305
                     /locus_tag="KPB88_002441"
     CDS             820..1305
                     /locus_tag="KPB88_002441"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG0054710.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1400..1608
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1672..3387
                     /locus_tag="KPB88_002442"
     CDS             1672..3387
                     /locus_tag="KPB88_002442"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0054711.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLVSQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3509..4954
                     /locus_tag="KPB88_002443"
     CDS             3509..4954
                     /locus_tag="KPB88_002443"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0054712.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMLLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5394..6875
                     /locus_tag="KPB88_002444"
     CDS             5394..6875
                     /locus_tag="KPB88_002444"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG0054713.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7096..7251
                     /locus_tag="KPB88_002445"
     CDS             7096..7251
                     /locus_tag="KPB88_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054714.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7396..8793
                     /locus_tag="KPB88_002446"
     CDS             7396..8793
                     /locus_tag="KPB88_002446"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG0054715.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEYKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGTRIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8778..9191
                     /locus_tag="KPB88_002447"
     CDS             8778..9191
                     /locus_tag="KPB88_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG0054716.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9297..10055
                     /locus_tag="KPB88_002448"
     CDS             9297..10055
                     /locus_tag="KPB88_002448"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG0054717.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10068..10805
                     /gene="rlmB"
                     /locus_tag="KPB88_002449"
     CDS             10068..10805
                     /gene="rlmB"
                     /locus_tag="KPB88_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG0054718.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            10808..11335
                     /locus_tag="KPB88_002450"
     CDS             10808..11335
                     /locus_tag="KPB88_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG0054719.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11400..12047
                     /gene="sigH"
                     /locus_tag="KPB88_002451"
     CDS             11400..12047
                     /gene="sigH"
                     /locus_tag="KPB88_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG0054720.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12121..>13055
                     /gene="tuf"
                     /locus_tag="KPB88_002452"
     CDS             12121..>13055
                     /gene="tuf"
                     /locus_tag="KPB88_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0054721.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEV"
BASE COUNT         5433 a         1353 c         2591 g         3678 t
ORIGIN      
        1 taactattta tacaactgaa aatttaatat ataattaatc agatggttaa aataaaaata
       61 ttaaaagttt taataaatga tggttaaaaa taaaatacga ggtgatacca tgtatagtgt
      121 tataattgtt gcagcgggaa gcggaaggag aatgaactta gatataaaca aacagttcat
      181 aaaattaaga gaaaaagaaa ttattgctca tacaattcaa gttttttatg aaaatataaa
      241 tatagatgaa atagtggtat gtataaaaaa agaagaagag gattttttta aagaaaatat
      301 aataaataag tataacttta aaaatataaa aatagcatat ggcggaaaag aaagacagga
      361 ctcaatatat aatggtttaa aaaaattaga tgaaaattgt gatatagtac ttatccatga
      421 tggagcaaga ccatttgtcg accatagaat aatcaacgaa tctataaagg tagctaaaga
      481 aaaaaaggct gtggttgtag gtgttccagt aagtgatact ataaagatag tgtcagatgg
      541 aacagtacaa gaaactccag aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt
      601 tgaatataat ctcataatag atgcttatga acaagcatat aagaacaatt attatggtac
      661 tgatgattca atgttggtag aaaatatagg tcaaagtgtc actatggtaa tgggttctta
      721 tgaaaacata aaaataacta gtccagaaga tttaaatata gcagaacaaa tattaaatat
      781 ggaaaaaaga gatgaagtta acagtagaag gagaattata tgagaatagg attaggatat
      841 gatgtacaca aattaacaga ggatagaaaa cttataatcg gtggtgtaga gataccacac
      901 gataaaggtt tgttgggtca ttctgatgct gatgtattga tacatgcaat aatggattct
      961 attttaggtg ctctagcatt aggagatata gggaagcatt tcccagatac agatgaagaa
     1021 tataagggag cagatagtat gaaattatta gaacatgtgt ataatctaat aacaagtaaa
     1081 ggatataaaa ttggtaatat agacagtact ataattgctc aaagccctaa gatggctcca
     1141 tatattgaga gtatgagaag taatatatca aaagttttaa acacagacat tgataatata
     1201 aacataaagg caacaacaga agaaggtctt ggttttacag gagcaaaaca gggaatagct
     1261 tcacaaagta tttgtttatt attgttgaca agtcagaata attaatatat tattagtaat
     1321 aagtttaatg gatatcaagt aataggccta tatagaatat ctataatgta tgaatttaag
     1381 tgaaattaat ataaatataa aattagctat gataaagaaa tagtaaaatg atgaaatctt
     1441 cacagagaga aaacggtggt gagagttttc aagaggaagt ctttgaacct gtcttttagc
     1501 ttaaaggtat aaaaatcttt acggtaggaa ccgttatatt cccaaaagag agctattcat
     1561 atagctaact agagtggtac cgcggaaatt aaacctttcg tctctatact tgtatagaga
     1621 tgagaggttt tttatatttt caggaagtta atattcagga ggaatctatt aatgaaaatg
     1681 tcaaaaatgt ttatgccgac attaaaagaa atcccagcag atgctgagat tacaagccat
     1741 caattaatgg ttaggtcagg catgataaaa aaaatgactt caggtgttta taaccaatta
     1801 ccaatggggt taagagtgtt caaaaaaatt gaacaaataa ttagagaaga attaaataaa
     1861 aaagattgtc aagaaatact ttgtgccgcc ttacttcctt ctgaattatg gaaagagtct
     1921 ggaaggtgga ctgctatggg tgaagaaatg tttagactaa aagacagaac agaaagagag
     1981 tactgtcttg ggccaacaca tgaagaagca ttcactgata taataagaca agaaattact
     2041 tcatataaac aattgccact gaacttgtac caaatacaag taaaatatag agacgagaga
     2101 agaccaagat ttggtgttat gagaactaag acttttacaa tgaaggatgc ctatagtttt
     2161 gatgtagatg ataaaggttt agataagtca tatcaagata tgtttgatgc atatgtaagt
     2221 atatttgata gatgtggatt ggagaatagc cctgtacagg cagattcagg agctatagga
     2281 ggttctacat cagccgaatt tatggttaag tcagaagttg gtgaagatga agttgtattt
     2341 tgtagtggat gtgattatgc agccaatgta gaaagagcag aatcttgtaa tctagtatca
     2401 caaaaagaag aaatgaaaga attagaagag gttcatacac caggagcagc tacaataaaa
     2461 gagttagaag aatttttaaa aacttctcca gataagtttg ctaaaacttt agtatatgag
     2521 gcagatggaa aaacagttgt agtcgttgta agaggagata gagaagttaa tgaaatcaaa
     2581 gtatctaatg ctataggttc tgttatagag tttgctcttg ctacagatga tgtagtgaga
     2641 aaagtaacta atgcagaagt aggatttgca ggtccaatag gtataaatgc tgactatgta
     2701 tttatagata aagaaattgt tgaacaaaga aacatagttg ttggggctaa taaaactgag
     2761 taccatataa aaaatgctaa ctatggaaga gattttgaag gaatagttgg agactttaga
     2821 aatgttcaag aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat agctagaggt
     2881 gttgaagtag gacatatatt taaactagga actaagtact cagaatctat gaatgcaaac
     2941 ttcatagaca aagatggaaa atcaaagcct atagtaatgg gatgttatgg aataggtgtt
     3001 gaaagaacag cagcagctat aattgaacaa cataatgatg agaaaggtat aatatggcct
     3061 ttgtcagtag ctccatacca tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa
     3121 atatcaatag ctgaaaatat atataatgat ttgcaagcta tgggtgtaga agttttatta
     3181 gatgacagag atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat aggaataccg
     3241 atgaggatta cagtaggtaa aaatattaat gaaggtaaag tagaatttaa acttagacat
     3301 aaagaagata aagagataat tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt
     3361 atcaggaata atgttaggtt aggataataa cttaaataag atattgtata gataatcaaa
     3421 gttgtacttt atagacacca ttttatctat atataaggat ataaaatggt gtttttttat
     3481 aaaacaaata aatgaggagg taattgaaat ggcaaaaaat gaaaaacaat ttgttgaaga
     3541 aataacaaag atggaagatg attttccaca atggtataca gatgtaataa ctaagactga
     3601 cttagttgat tatgcaccag taaaaggttt tatggttgta aagccatatg gatatgcatt
     3661 atgggaaaag atgcaagaat ttatggataa aaaatttaaa gaaactggac ataaaaactg
     3721 ttattttcca ttattaatac cagagagttt attaaacaaa gaagcagaac acgttgaagg
     3781 ttttgctcca gaagttgcat gggtaacaca tggaggaaat aaaaaattag aagaaagatt
     3841 atgtgtaaga cctacttcag agactataat atgtacaatg tatgcgaaat ggttaaaatc
     3901 atatagagaa ttaccatatc tttataatca atggtgctca gttgttagat gggaaaaatc
     3961 aactagacct ttcttaagaa catctgagtt tttatggcaa gaaggtcata cgttacatga
     4021 aacagcagaa gaagcacaag aagaaacaat acaacaatta gaagtatata aagcgctatg
     4081 tgaagaatta cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg agaaatttgc
     4141 aggtggtgaa agaacatata ctatagaagc tatgatgcat gatggtaaag cattacaatc
     4201 aggtacaagt cacttcctag gtcaacactt tactaaggct tttgatatta cttttgcaga
     4261 tagagaagga aatttagcta atccatacca tacatcttgg ggagcatcta caagacttat
     4321 aggtggactt ataatgactc atagtgataa cagaggttta gtattaccac caagagtagc
     4381 tccaattcaa gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt
     4441 tgataaaata tatgctgatt taaaagctaa gggagtagct gtagaagttg atgatagaga
     4501 taactataca actggatgga aatttaatga gtgggaaatg aaaggtgttc cagtaagagt
     4561 tgaaattgga ccaaaagata tagaaaataa tcaagctatg gtatttagaa gagatacttt
     4621 agaaaaggat tctatgcttt tagaaggatt agctgatgct atatgtgatt tatttgatgt
     4681 tatacacaat gatatgtttg aaaaagcaag aaaacataga gaagacaata catctatagt
     4741 tgaaaatatg gatgagttta gaaaagcttt agaagaaaag ccaggattca taaagactat
     4801 gtggtgtgga gatgcagaat gtgaagctaa gattaaggaa gaaacaggag caactataag
     4861 atgcttacct tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc
     4921 agatagtatg gtagtaatgg caaaagctta ctaaaataca tttaaataaa aactggggta
     4981 gaaagtattt aatactttat cccagttttt ttgtttttta tgagtaataa aatgtatttg
     5041 taactatgtc ataagataag tcatagatac ttatattaaa aagaatataa tagagaacta
     5101 tttttaataa ttacatatta atgaaagcaa aatatttttg ttatatagat tttcaataaa
     5161 gcaaacaata aatataaatg aatattagta aggagtgctt ttaattttta gaaaataaat
     5221 gacttaaaag aaagatttat gaaattaaaa ctaggatttt ctgatataat aaacatgtgt
     5281 atttttaaat aaaaaggata ttattattag atttgattaa aaataaactt gcaaaacaat
     5341 gtactagttt aatattatag ttatattgaa taaaatatca ggaggaagta aaaatgaatg
     5401 taagagtaag gtttgcacca agtccaacag gatttgtaca tataggtagt ttgagaactg
     5461 ctttatataa ctatttattt gcaaaaaaaa tgggcggaga atatatatta agagtggaag
     5521 atacagacca aagtagatta gtagaaggtg ctatagagaa tatgcttaat gctatgaaat
     5581 gggctggggt caaccatagt gaaggtgtta tattagatga tagtggaaaa gtagtacaaa
     5641 aaggagaata tggtccatat attcaatctc aaagattaga tatataccaa gaatatataa
     5701 agcaactttt agatagtgga aaagcttatt actgtttctg tacaaaagaa agactggatg
     5761 aagtaagaga tgctcaaaga gcagcaggag aaacagctaa atatgatggt cattgtaaga
     5821 atctttctaa agaagaagta gaagcgaata taaaagcagg aataccatat gttataagat
     5881 taagattgcc agagaatcat acaattaagt ttacagactt agttagagga gatatggaat
     5941 ttaatactaa tgacttagat gaccaagtat taatgaaaac agatggattt ccaacttatc
     6001 attttgcagt agttgttgat gactatttga tgaaaattac acatgttata agaggagaag
     6061 aatgggtatc atctactcca aaacatgttt atttatatga agcttttgga tgggaagcac
     6121 ctgtatttgt gcatttacca aatatactta ataaagaaaa gaaaaaatta agtaaaagac
     6181 aaggtgatgt agctgttgaa gactttaaga aaaaaggata tttaccagag ggattagtaa
     6241 actatgtagc attagttggt tggtctcctg aagataatca agaattgttt acaatggagg
     6301 aattagaaaa agccttctct gtagaaagag tatctaaaag tggtggagta tttgatactg
     6361 aaaaattaaa ttgggttaat cagcactata taaaagatgg tgatgatgct tatctaacag
     6421 acttagcaat accattttta atagaagatg gatttataac agaagaagaa gctactaata
     6481 aatatgattt cttaaagagt atgatatcag tacttaaaga gaaattacaa tatgtaaaag
     6541 aagttactga acatgcaagt atattttttg gagataaaat agaggtagaa actgaagagg
     6601 gaagagagtt cttaagatta gaacatatac ctactttaat agatgctttg agagaaaaaa
     6661 tagaaaaaac agaggtatta aatgctgagt ttgttcaagc tatgcttaaa gaaatacaaa
     6721 aagagtacaa aataaaaggg aaaaatttat ttatgggctc aagaataatt ttgacagggc
     6781 aaatgcatgg tccagattta ccaaaagtaa tggaagtttt aggaaaagaa acttgcttaa
     6841 atagaatagc ttatgtaaaa aataatatat tatagtcata aaagtccgct aatttagaag
     6901 cggacttttt ttgtgttagt atttggtgaa aatatctgct taagaaaatg aaggtattta
     6961 aggtaataaa tttaatatat aaaaagcaaa gcaaaaatat aaataaaata tgaataataa
     7021 actaataaaa tattaagcat agaagaaggg aatctattat tttagtttga tttaattatt
     7081 aagggggtaa ttactatgta tagtaatatt gatgatgtta aaaaggaatt aaaggagcta
     7141 tgcttagaat atgttacaat tttagaaaaa ttaaaagatg aaaaaatgat tacagaagaa
     7201 acttttgaaa agtgctcttc tcaaaaaaaa atatttttag aagaacaata gaggatggta
     7261 atagtattat tattatgtag tttgaaagat agtacgaaaa atgatagtaa tactattatt
     7321 tattgcaatt ttcaactata atataatata taatgttaag aagataattt ataaaaatac
     7381 agtgaggtga tgagtgtgaa agtttataat acattgacta gaacaaaaga agaatttgtt
     7441 ccattagaag aaggaaaggt aaaaatgtat gtgtgtggtc caacagttta taactatata
     7501 cacataggta atgcaagacc atttataata tttgatactc taagaaggta tttagagtat
     7561 agaggatatg atgtaactta tgtacaaaac tttacagatg ttgatgataa gattataaat
     7621 agaagtcatg aggagggaat atctcctgaa gaagttgcgg ctaagtatat aaaagaatat
     7681 tttgtagact gtgatgggct aggaataaaa agagctactg ttcatcctca ggttacagat
     7741 aatatacaac agattataga gtttataaaa gagttggaat ataagggata tgcatatgca
     7801 gtaaatggtg atgtatattt tgatacaaat aagtttgaag gttatggaaa actttcaggt
     7861 caaaaacaag aagatttaga ggcagggact agaattgagg ttaatgacca aaaaagacat
     7921 cctatggact ttgttctttg gaaggcaaaa aaagaaggtg agccaggatg ggatagccct
     7981 tggggagaag gtagaccagg atggcatata gaatgttctg ttatgtctaa gagatattta
     8041 ggtgaaacaa ttgatataca tgcaggtggg caagatttaa cattcccaca tcatgaaaat
     8101 gaaatagctc aaagtgaagc tagaagtgga aaaatattct caaaatactg gatgcataat
     8161 ggatatataa atataaatga tgaaaagatg agtaaatcaa aaggtaattt ctttacagta
     8221 agagatatat caaaattata tgatttagag atagtaagat ttttcatgtt atcagcacat
     8281 tatagaaatc cagtaaactt tagtgatgag atgttaaatc aagctaaagc aggtcttgaa
     8341 agactatata atactaaaga aaaattagaa tttacgctaa gtaatttagt agaatctccg
     8401 ttaacagaaa aagaagtaga gcttgttaaa gaattagatg actttagaca aaaattcata
     8461 gatgctatgg atgatgatgt taatacagca gacgcagtaa gtgtaatatt tgaattagct
     8521 aagttaataa attctaatgt agatgaaaat tcttctttgg agtttgctaa aaaatgctta
     8581 gatgaattta atgaacttac aggagtatta aatattgtaa ataagaaaaa ggatactgta
     8641 ttagataaag acattgaaga attgatacaa aaaagaactg atgcaaagaa aaataaagag
     8701 tttcaattag ctgatgatat tagacaacaa ttactagata tgggaattgt attagaagac
     8761 acaagacaag gtgttaagtg gaaaaggata taaaaatgga aaaaactgaa ttagttacta
     8821 tgtcaccatt agttttagca tacttaggtg atacagtgta tgagacatat ataagagaat
     8881 atttaattag acagaataca caaagaaaag ttaatgattt gcataaacta gctataaagt
     8941 atgtaaaagc taaagctcag gcaactataa ttcatgaaat tgaaattgag ttaactgaag
     9001 aagagagtaa aatttataaa aggggaagaa atcaaaagtc aaatacttct cctaagaatg
     9061 cagatattat agactacaaa catgcaactg ggtttgaggc attagtggga tatttgtatt
     9121 taaataatga aatagagaga cttcaatata ttatcaataa aggaatcaaa attattgaaa
     9181 gagatatgta aatattaaag agtgttgtct taataataag taataaggca acactttttt
     9241 tataactaat gtataataaa tatatagaaa ttagtaatga gaaaggataa aaagttatga
     9301 aagtaaaatt aatatcacat acacctgaac cagaaaaagt aatagctatg gcagctaagc
     9361 tatgttattc accagttggg acagacgaaa tagaaaaaga cttaacagat gaaagtatag
     9421 agaaattttt aaatatgtta ttaagtatag gtcatgggtc aatattagaa catgcttcat
     9481 ttacattttc aatagaaggt atttcaaggg cttgttcaca tcaaattgtt agacatcgta
     9541 tagctagttt ttcacaacaa agtcaaagat atgttaaatt agaacaattt gagtacataa
     9601 ttcctccaga aatagaaaaa atagaaaaag caaaagagtt atttatagac tccatgaaaa
     9661 aagaccaaga gaattatgat aagttagttg aaatattgtt tgaaaatcat tataatgacc
     9721 taataaaaaa cggtaaaaat gagaaaacag ctaaaagaca agctgaaaag aaagcaatag
     9781 aagatgctag atacgtattt ccaaatgcat gtgaaacaaa aatggtattt actataaatg
     9841 ctagaagttt gtttaatttc tttgaacata gatgctgtga aagagcacag tgggaaataa
     9901 gaaatttggc tgtggagatg ttaaaagagg ttaaaaaggt tgctcctata ttatttaaaa
     9961 aaactggtcc aagttgtgta aatggaagtt gtccagaagg aactatgact tgtggagata
    10021 tagttcaagt tagagaaaaa tttaaggcct tgtaggaggt gaataatttg gcgagtatag
    10081 aaggaagaaa tccggtaata gaagcaataa aaagcgacag agaaatagat aaaatattaa
    10141 ttgcaaattc agctaaagaa ggttcaatta agaaaataat aggaatggct aaagataaga
    10201 atataattat tcagtatgtt gataaacata agctagatga agtaagtaca agtcattcac
    10261 atcaaggtgt aatagcttat gcaagtgagt ataagtatta tgaattagat gagctaatag
    10321 atttagcaaa gaataaagat gaagacccat ttttcattat attagatgag ataacagacc
    10381 cacataattt gggttcaata ataaggacag cagatgcagt aggagcacat ggagttatca
    10441 ttccaaaaag aaggtctgta cacataactc ctgtagttgc caaagcatct gctggagcag
    10501 tagaatatat gccagtttgc aaggtaacca atatagttaa tacaataaaa agactcaaag
    10561 aagaagggct gtggattgct gcagctgata tggatggaga aactttttat aaacaaaatc
    10621 ttactggtcc tcttggtgta gtaattggaa gtgaaggttt tggaatatct agattggtta
    10681 aacaaaactg tgactttata gtaaaaatgc caatgatagg taatgttaca tctttaaatg
    10741 catctgtagc aggtggaata cttttatatg agatattcag acaaagactg gataaaagta
    10801 aataggcatg aaaaaaaata taaatcatta cttaataata gatggctaca atattataaa
    10861 tgcgtgggac aatttgaaag aactggctaa agaggactta gaagattctc gagaaaagct
    10921 tattgatgac attatagaat tttctgagtt tatgggttat aagactataa tagtttttga
    10981 tgcttataat gttaaaaatt ctcgagaaaa agttgaaaaa agaaaacaca taacaatagt
    11041 ttatacaaga gaacaccaaa cagcagatag ttatatagag aaatttataa cttctttatc
    11101 aaagtatgat gatgtaaaag tagccactaa tgactatgca gaacagcaaa tgattttagg
    11161 aaaaggtgct actagaatgt ctgcaagaga attaaagtta gagttagatc gttccaagaa
    11221 taaaatgaag gaaaaaaata tcggtttgag gaaaaaaatt cagcgaaatt ggttggagga
    11281 aaggttagat aaagaaacat tgtcgaaact tgagaacatt cgtagaaagc gttgaaatta
    11341 ctaggttctt tagactataa tatacctata aatttgtttt atatatgggg gagatttaca
    11401 tgttggtagc aaaagaaaaa agttatgagt tagtggacaa ttgtcagcag gatgagtata
    11461 acattgtatt aagagcaagt gaaggggata aaatagcact agagtacatt attactaaat
    11521 acagaaattt tgtaaaggca aaagcaaagt catatttttt aattggagca gataaagaag
    11581 atataataca agaaggaatg ataggccttt ataaggcagt tagagatttt gatggaagta
    11641 agaccaattc atttaaatgt tttgcggaga tatgtataac aagacaaata ataactgcta
    11701 taaaaactgc aacaagacaa aaacatatac ctttaaattc atatgtttct ttgaataaac
    11761 caatatatga tgaagagtca gacagaacac ttttagatat tatagctaca agtatagtaa
    11821 cagatccaga agagcttata attagtaaag aagagttaaa aaatatagaa tcaaaaatga
    11881 acgaattgtt aagtgacttg gaattagaag tactagaatt atatttaaat ggaaagtcat
    11941 accagtttat agcggacaag cttaaaagag atgtaaagtc aatagataac gctttacaaa
    12001 gggttaagag aaaattagaa aaacatcttg aaaacagaaa tgattaatag taaaatactt
    12061 accataagct tattgtgagc aaaattaatt tatatcgaaa gataagggag gaaattcaaa
    12121 atggctaaag ctaaatacga aagaacaaaa cctcatgtta atatagggac aataggacac
    12181 gtagaccacg gtaaaactac attaacagca gcaataacaa aaacattata tgacagatat
    12241 caattaggag aagcagtaga tttcgcaaac atagataaag ctccagaaga aagagaaaga
    12301 ggaatcacaa tatcaacagc acacgttgag tatgaaacac caaatagaca ctatgcacac
    12361 gttgactgcc caggacatgc tgactacgtt aagaacatga taacaggagc agcacaaatg
    12421 gacggagcaa tattagtttg ttcagcaaca gatggaccaa tgccacaaac aagagagcat
    12481 atactattat caagacaagt tggagtacca tatatagtag tattcttaaa caaatgtgac
    12541 atggtagatg atgaagagtt attagagtta gtagagatgg aagtaagaga tttattaaca
    12601 gaatatgatt tcccaggaga tgacactcca atagtaagag gttcagcatt aatggcatta
    12661 gaagatccaa agagtgagtg gggagataag atagtagaat tattcgagca aatagatgag
    12721 tatataccag ctccagagag agatacagat aaaccattct taatgccagt agaggatgta
    12781 ttctcaatca caggaagagg aacagttgca acaggaagag tggaaagagg agtactaaaa
    12841 gtacaagacg aagtagaatt agtaggatta acagaagcac caagaaaagt agtagtaaca
    12901 ggagtagaga tgttcagaaa attattagac caagcacaag caggggataa tataggagca
    12961 ttattaagag gagtacaaag aaacgagata gaaagaggac aagtactagc aaagactgga
    13021 tcagtaaagg cacacacaaa gtttacagca gaagt
//
LOCUS       DAELJO010000107        13009 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELJO010000107 DAELJO010000000
VERSION     DAELJO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.107"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            49..1086
                     /locus_tag="KPB88_002453"
     CDS             49..1086
                     /locus_tag="KPB88_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0054722.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            1167..1532
                     /locus_tag="KPB88_002454"
     CDS             1167..1532
                     /locus_tag="KPB88_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054723.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            1537..1854
                     /locus_tag="KPB88_002455"
     CDS             1537..1854
                     /locus_tag="KPB88_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054724.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            1902..3506
                     /locus_tag="KPB88_002456"
     CDS             1902..3506
                     /locus_tag="KPB88_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054725.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            3630..5258
                     /locus_tag="KPB88_002457"
     CDS             3630..5258
                     /locus_tag="KPB88_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0054726.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            5523..6872
                     /locus_tag="KPB88_002458"
     CDS             5523..6872
                     /locus_tag="KPB88_002458"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG0054727.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVMSKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7028..8107
                     /locus_tag="KPB88_002459"
     CDS             7028..8107
                     /locus_tag="KPB88_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0054728.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            8452..9360
                     /locus_tag="KPB88_002460"
     CDS             8452..9360
                     /locus_tag="KPB88_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0054729.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9365..11734
                     /locus_tag="KPB88_002461"
     CDS             9365..11734
                     /locus_tag="KPB88_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG0054730.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKRDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDTRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            12060..12863
                     /gene="proC"
                     /locus_tag="KPB88_002462"
     CDS             12060..12863
                     /gene="proC"
                     /locus_tag="KPB88_002462"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0054731.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5232 a         1388 c         2451 g         3938 t
ORIGIN      
        1 taataacaaa ttgtacttta atttaataat aagggagtgg aagtcatatt gttagggaga
       61 aatgagctat gcccttgtgg aagtggcaaa aaatacaaga aatgttgttt aaataaagat
      121 attgtttcag aaagagcatg cagaaaaact gaactttcac aaaaacaata tacagattta
      181 tattcaaagt tatatgatta ttcaagacag gataagttta aagaagaatg tgaaaaagct
      241 aaagaaatgt tttatataat gcaaaatgat agtgtaaatg aaaaatttga aagatttttt
      301 aatacttatt taatacaaga ccacataatg gaaaataaaa aggttatgac agttggtttt
      361 cttgaagaaa atggtgcaaa cctaagtcaa tcagaagtat caatattaaa aagtatgttt
      421 gaatcatacg taagtattta tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag
      481 gactgtttaa gtggaaatga actttgtaca gaagatgtaa aacttctgag aagttttaaa
      541 atcggaagtt gtatgatagc tagaatagtt gatattgaag gaactaacat attgatagat
      601 ataactataa gtatatcaaa tgaggttaaa gatataattt taaaggatat tatgaatcta
      661 ttcaatcaat atcaagatgt atacaaagat atgaagtcgt tcttaattta tcatacacat
      721 atactataca agtatataca acagctttta gacccaagta tagctgaata tttaaagaga
      781 gaaagagata aaaacgatac aaaagttaat tcagaaaatg aattagctga agaagaatgt
      841 aaagttttag atactcttaa agaaaatgta gaaaaagaaa atatagatgc atgtatagag
      901 ttttggaatg aatataagaa aaatcatgaa aatataaaag gagctgaaaa tggttgggca
      961 gctgctgttg aatactatgt taaaaaagaa gcaggacaac ctataactca agttcaaata
     1021 tcaaagaaat acgatataag ctctagtaca ctaggaaaga gatacaagga tttaaaaata
     1081 tcttaattaa ttataaaaag ctttaagtca tagataaaga atctatagta taatatatgt
     1141 ataatgttta gaaagaggta ttaataatga gtcttagttt aggaaatgca attgctgtat
     1201 caacgaactt tataaaatct aacccagctg tacctgtgta caacggagaa gaaatatatg
     1261 cagttattta tcatatagaa ataggatata ataatggtgt atcacctgat accaaacaat
     1321 atataataat agactataaa gagtttgatt tatctatgga taaaaaaaat gaactaataa
     1381 atattgctaa agagtactgt aaatctaaag gtattgtaga aaacgaaaac cttgaaataa
     1441 accttttaga caatgaagaa gcagagaact tcttagaaaa ggtgtttaat aatatgaagg
     1501 ttataagagg tcttataact aaaaaaggat aaataaatgg actatatagg aatagaaaat
     1561 ataacacctt atgaaaatac atatgaattt agtgtatatg aatatgatga tgaaatcacc
     1621 ttaggtagtg aaaagttata tgtatgtgaa ttgagggttg tattgattaa agttaattct
     1681 ctgtatgttg aaagattgca taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa
     1741 aaagatttag ataaaacact tgttgtaaac aaaataaaga attttgtgct agatgagatt
     1801 tgggtagaaa atctagtaaa agagaatata gaagttatat ttgtagaaag ctaggaataa
     1861 ccctagcttt tttatattac aatctaagag aggaaaacaa tatgttaaga gtatctaatg
     1921 taaaacttgg aattgataaa gatatatctt tattaaaaga tgttatactt aagaagctaa
     1981 aaataaaaga aaaaaactta gtcaaatata gtatatataa agaatctata gatgctagaa
     2041 aaaaaggaaa aatggaattt gtttattctg tagatgtaga agttaaagat gaaaataaaa
     2101 tacttaactc aaaaataaaa gatgtaacaa aaataaagga aatcagatac gtaaatgtac
     2161 ctgtgggtga taaaaaatta aagaataatc ctttggtaat tggtagtgga ccagctggtt
     2221 tatttgcagg tctgctttta gctcaaatgg gttatgaacc aatcatactt gaaagaggtc
     2281 ttgatgtaga cagtagaacc aaagatataa gtgatttttg gacaactaga aaattaaaaa
     2341 ataattcaaa cgttcaattt ggagaaggtg gagcaggaac tttttcggat ggtaaactta
     2401 caactagaat aaaagatata cgatgtagaa aagtattaga agagttagtt cagtttggag
     2461 caccagatga aatattatac tcacataagc cacatgttgg aacagatata ttaaaaaatg
     2521 tagttaaaaa tataagaaat gaaataataa gtttaggtgg tcaagttaga tttaattcaa
     2581 aagtaacaga tatagttgta tcaaatgatt ctatacaatc tgtaataata aatgatactg
     2641 aaaaaataga tacagataca attatattag ctgttggaca tagtgcaaga gatacttatg
     2701 aaatgttgta ttcaagaggt ataaaaatag ttcaaaaacc ttttgcaata ggagctagag
     2761 ttgaacatcc acaagattta ataaataaag cacagtacaa ggatttttat aatcatccta
     2821 ggttaggagc tgcagattat agacttatag agcatgtttc aaatggaaga acagcatata
     2881 ctttttgtat gtgtccagga ggtagtgtca tagcatctgc atcaaatgat tttgaaatag
     2941 ttacaaatgg tatgagtgaa catgcaagag ataaaataaa tgcaaatagt gcatttttag
     3001 taaatgtaac gccagaagat tttaagagtg ataatccact agcaggtgtt catttccagc
     3061 agaagtatga aaaattagct tttgaattag gtggaaaaaa ttataatgca ccagttcagc
     3121 ttgttggaga ttttataaac aataagactt ctttgaattt aggaagtgtt gagccatcat
     3181 atagaccagg atatacactt acaaatttaa gtgaatgttt gcctagcttc gtaacagaga
     3241 ctatgagaga agcgtttata tctcttgata aaaaattgaa tgggtttgca atgtatgatg
     3301 ctgttttgac gggagtagaa actaggtcat catcaccaat aagaatagta agagatgaaa
     3361 atactttaga atcagtgagt attaagaatc tatatccatg tggtgaaggt gctggatatg
     3421 caggtggtat agttacagca gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa
     3481 aatacaccaa taatacaaaa atttaacata aacttaatga ttttttaata tttaacatga
     3541 atttaacatt atcactccta taatttgata aaatacaata ggttataaaa tttttagata
     3601 cagtaatctc tttacaagga ggaaataatt tgaacattat aataagtcta atgggtggat
     3661 taggtctgtt cttatatggt atgaacctta tgggagaagg tcttcaaaaa tctgctggga
     3721 caaagctaaa aaagataata aaactactta ctagtaacct atttatggga gttttggtag
     3781 gtacaggagt tacagcagta atacaaagtt caagtgctac tactgtaatg gttgttggtt
     3841 tcgtaaatgc aggaataatg actttaaaac aggcaatagg agttataatg ggtgctaata
     3901 tagggactac tgttacagcc caattggtat ctttcgactt aactggtatg gccccagtag
     3961 cattaggtgt aggtattata ctttatttat ttggaaataa gcctagaata aaaaatatag
     4021 ccgaaatact tataggtttt ggtatattat ttacaggtat ggattttatg aagatggcag
     4081 tggagccact aagagactat caaggattta ctgacttact tgttacattt ggtagatatc
     4141 cactacttgg attattattg ggatttggga ttacagcaat aatacaaagt tcaagtgcat
     4201 ctatgggtat gttggttgca ctggcagcag aaggtctagt gccattatca gcagcactgc
     4261 caattcttta tggccaaaat attggtacat gtgtaacttc actgttatca agtattggtg
     4321 ctaacaaaaa tgcacgaaga gcagctatga tgcatctaat cttcaatgtt ttaggaacag
     4381 tgatattctt aatattcttg aataaaccag tagtaagcat ggttacaagt tgggacccaa
     4441 gcaatgttgc aagacaaata gctaatacac atacattgtt taatataata tctgtattga
     4501 tacttttacc atttacaaat cttattataa aactagctat aaagcttgtt cctgatagag
     4561 caggtgatat agatgaagat gaaactaaaa ctattaaata tatagatgat agaatgatag
     4621 aaactccttc tatagctctt gcaaatacga taaaagaagc acttcgtatg ggtgagaagg
     4681 cgaaggaaag cctaaatgca tctatggaag ctttagtaga acattcaact gaaaaaatag
     4741 ataagacata taggcgagag agattaatca atgatttaca gaaagctatt ttaaattatt
     4801 tacttaaact ttcaaaagca cctttagatg atgattcaag agaagttgtg gatactttgt
     4861 ttaatacagt caatgatata gaaagaattg gtgaccacgc taaaaatata gctgaattat
     4921 cgcaagttgc aatagattca aatatttctt tctcagaaga aggacaatct gaacttgatg
     4981 ttatgtataa tagagttgtt tcagcatata cttatgcatt agaatcaatg agaactgata
     5041 atgttgattt agcttgtaag gttataaaaa ttgaagaaca agttgatata atggagaaat
     5101 cttgtagagc taatcacatg tatagattga acaataatct atgtagtata gaaaatggag
     5161 ttatttattt agacgtaata agtaatttag agagaatatc tgaccatgct gtcaatatag
     5221 cacaacaagt tatagcgaag agattaggaa atgattaaaa ataattaatt taaaataata
     5281 taaaatttaa aatagaggat aaataaaaga gaaactttaa atgatacgtt tagagtttct
     5341 ttttttgtaa atttatttga ctaaaatttt taaatttata catagttata gcatttagaa
     5401 gaatcgattt cctgataata aatgatgaga tatgtatgat tttgtggcaa acttttaaaa
     5461 aaaacaccta tattttgtgt tataatctct ttaaaacgaa tatgctgaaa agagggattt
     5521 aaatgggaaa aataaatttt gattatagta aagctacaga gtttttttgt caaaacgaaa
     5581 tagatgtcat gcaaccatat gtagatgttg cgcacgacat gttacataat aaaactggtt
     5641 taggaaatac ttttttagga tggatagacc ttcctaaaaa ttatgataaa gaagagtttg
     5701 acagaataaa aaaatctgct gataagataa aatcagactc agatgttctt ttggtaatag
     5761 gtataggagg ctcttattta ggttcgagag ctgctataga tatggtaagt cattctttta
     5821 ggaatggact taaaaaagag caaagaaagg ctccagaagt atattttgta gggcataata
     5881 taagctcaac atatattatg gatttattag atataattga aggaaaggat atttctgtaa
     5941 atgtaatgtc taaatcagga actactacag aaccagctct ggcttttaga atatttaaag
     6001 attacttaga gaaaaagtat ggtaaggaag aagcaagaaa gagaatttat gcaacaacag
     6061 atgcaagtaa aggtgcactt agacagttag ctacagaaga aggatatgag acttttgtaa
     6121 taccagatga tgtaggcggt cgtttttctg tattgacagc agttgggtta cttcctattg
     6181 ctgctgctgg acttgatatt gatgctatga tgaaaggcgc aaatgatgct agagaagcat
     6241 ttcaaaatcc tgacttaaaa agtaatgata gttatagata tgctgttgct agaacaattt
     6301 tacatagaaa aggaaaagat gtagagttat tagtgaacta tgaacctcaa ctacattatg
     6361 taagtgagtg gtggaaacag ctttatggag aaagtgaagg aaaagaaaat aaaggtttat
     6421 tccctgcttc tgtagatttc tcaacagatt tacattctat gggtcagtac atacaagatg
     6481 gaaaaaggct tttatttgag acagtactta atgttgaaaa ctgtaagaga aatataacta
     6541 taagttcaga ggaagtagac ttagatggtt taaactattt agcaggtaag actgtagatt
     6601 ttgtaaatca taaagctttt gaaggaactc tattggctca tacagatgga aaagtaccta
     6661 atttagtaat aaatatacct caactagatg agtataattt tggatattta gtttatttct
     6721 ttgagaaggc ttgtggaata agtggatatt tattgggagt taatccattt gaccaaccag
     6781 gagtagaagc ttacaagaaa aatatgtttg ctttactagg caaaccaggt tatgaaaaag
     6841 aaaaggaaga gctagaaaaa agacttaaat aattttaaat agttaaggtt taattaagat
     6901 taaaataata taatgaaatc atcaaaaagg agtttactca aaatagatga tttcattttt
     6961 tttatgaaaa attgggaaaa atatattttg ataatttata ttatattaag atagggagtg
     7021 gtatgagatg tcaagaagaa agaaagggat aagtttagta atattagttg ctataataag
     7081 ttcaatactt agtagttttc taacaataat tttagttaag gacaatttag taagtaaatc
     7141 tacaggaagt tctacgccga tagttgtaaa tgatgatggt aagagtcaaa atatttatca
     7201 agcagttgca gaaaaagcta cgccatctgt tgtaggtatt acaacaacta gtgttgatac
     7261 tagtaatatg tttgcaatac caactgaaac acaaggtgta ggaactggaa tcatagttga
     7321 ttcaaatgga tatatactta ctaattctca tgttatatca gatggtcaag ctacaagtgt
     7381 aaatgtatta ttcaatgatg gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca
     7441 acttgattta gctatagtta aggttgataa aactggacta acaccagcag aatttgctga
     7501 tagtgacaaa gtaaaagttg gagatatatc aatagcaata ggtaatcctc ttggattaga
     7561 ttttcaaaaa acagttactc aagggataat aagtggatta gatagaacta tacaaacaga
     7621 aaaaactaat atgactggtt tattacaaac tgatgcaagt ataaatgcag gaaatagtgg
     7681 aggaccattg ttaaatcaaa aaggtcaagt aattggtata aacacagcaa aagcttctca
     7741 agcagaagga ttaggatttg caataccaat caatacagct aagagtatag ttgaagaagt
     7801 aatcaaaaat ggaaaatatg aaaaagtaac tttaggcata aaaggtacag atgtatcaaa
     7861 ttatgaagca gcaacaggta caaaattaag tactgataaa ggtgtttatg ttgcagaagt
     7921 aatatctggt tcatcagcag aaaaagctgg agtaaaggta ggggatatta taactaaagt
     7981 aggagataca gatataacag gtatgaatga tttaaataaa aagttgtata ctttctcaaa
     8041 aggtgctagt acaaaaataa cagtaaatag aggtggaaaa gcagtaacaa taaatgtgaa
     8101 tttctaaata tgaccagaaa gatagagact acatggtaaa gtgtagtctc tatttttatt
     8161 taatttttat agtatataca tctaaattaa ttaaaatgat gtattgttta tagtttattg
     8221 ctatattttc ttataaaaat aaatttaata aatatttaaa tttaatttac aaaaacaata
     8281 taaatatcat aataaaataa aattttttaa tattactaaa aacatataga caaaataata
     8341 acaaatatag tattatgtaa ttgtagaaat gcttttcata ggtatattct atgaagatga
     8401 attgcataaa aatgaaaaaa tggaatggat attatttagg ggtgaaaaat catgaatcca
     8461 ttagttataa acttacaaaa atgtagcata catgatggtc cagggataag aagtacagta
     8521 ttttttaaag ggtgtccact tgaatgtgtt tggtgtcata atccagagag tcaaacatat
     8581 acaaaacaag ttttgtataa tgaagaaaga tgttcaaaat gtgaagcatg tataaatata
     8641 tgccctcata aggcaattta caaaggtgaa acaaaaatat gcttagacca agataagtgt
     8701 gagttttgtg aaacttgctt agactattgt gtaaacaatg caagagagat tgttggtcaa
     8761 gagtattctg taagagattt ggttaaagag atagagaagg atagaatttt ctatgaagaa
     8821 tcaggtggtg gagtaactct atcaggtgga gaagtaatgg ctcaagatat ggacttcatt
     8881 tgtggagtaa taaacatgtg caaatcaaag ggaatccatg tagcaattga tacatgtggc
     8941 tatgcaaagt cagaaaatta tgaaagagtt gctaaatgtg cagatttgtt tttatatgat
     9001 attaagctta ttgatgaaga taagcacata aaatttacag gaaaatcaaa tgacttaata
     9061 ctgaaaaatg ttaagatttt aagtgaatta ggggctaaca taaatataag aataccatta
     9121 attgtaggag taaatgtaga tgatgaaaat ttagaggtta aaaaaatgat agagttttta
     9181 aagcctttga atatacaagc agtaagtctt cttccatatc ataatattgg taaacacaag
     9241 tatgataaaa tttataaaaa gtatgaagga gaagaactac aaagaccatc agaagaaaaa
     9301 ttagaggaaa taaaaagact gtttgaagca agtaatttca atactaagat tggagggtaa
     9361 taaaatggca agaggaactt ttgagagaac taaaaaatta agagaagaaa gtattaatgc
     9421 agagccacac atatctattg aaagagcagt acttatgaca gaagcttata aaaaatatga
     9481 gggaagtgta gagattcctg tacttcgtgc tttatcattt aagcattata ttgagaatag
     9541 aactcttagc ataaatgatg gagaattaat agtaggtgaa aaaggtgatt ctcctaatgg
     9601 agcaccaaca tatccagaaa tttgttgtca tacaatggaa gatttagaag taatgcacaa
     9661 tagagatata ataaatttta gcgtatcaga agaagctaga aaaatacata aagaagaaat
     9721 aattcctttt tggaaaaaga gacaaacaag agataagata ataaatgcaa tgacacctga
     9781 gtggttagca gcatatgaag caggtatgtt tacagagttt atggaacaaa gagccccagg
     9841 acatacagtt tgtggagata caatatataa aaaaggattt ttagatttaa agagagacat
     9901 agaagcaaga ttaaaagagc tagattttct taatgattta gatgcatata ataaaaaagc
     9961 agatttagaa gctatggcaa tagcttgtga tgcaatggtt atattaggta aaagatatgc
    10021 tgagaaagca agacaaatgg cagaagaaga aacagatgaa gctaagaaac aagatttatt
    10081 actaattgct gaaacttgtg atgttgtacc agctcacaaa ccagaaactt atcatcaagc
    10141 tatacaaatg tattggtttg ttcatatagg ggttactact gaacttaata tatgggatgc
    10201 ttttactcca ggaagacttg accaacatct aaatcctttc tatgaaagag atgtagaaaa
    10261 tggaatacta gatagagata gagcacaaga attacttgaa tgtttatggg ttaaatttaa
    10321 caatcagcca gcaccaccaa aagttggtat aacattaaaa gaaagtagca catatacaga
    10381 ttttgcaaat ataaacactg gtggaataaa tccagatggt caagatggtg ttaatgaagt
    10441 tagttatata atacttgatg ttatggatga aatgaaattg atacaaccta gttcaaatgt
    10501 tcaaataagt aaaaagactc ctcaaaaatt cttaaagaga gcttgtgaaa tatcaagaaa
    10561 aggatggggt caaccagcat tttataatac tgaagcaata gttcaagagc ttatggaagc
    10621 tggaaaaact atagaagatg caagacttgg tggaaccagt ggttgtgttg aaactggatg
    10681 ttttggtaaa gaagcatatg ttctaactgg atatatgaac attccaaaaa tacttgaatt
    10741 aacattaaat aatggatatg acccaattag taaaaaacaa ataggtattg aaactggaga
    10801 cactagaaat ttccaatctt atgaggaact atttgaagca tttaaaaagc aattacatta
    10861 tatgattgac ataaaaatag aaggaaatgc tgtgatagaa aatatatgtg caaaacacat
    10921 gccttgtcca cttatgtcta caatagttga tgattgtata gaaaaaggca aggattacca
    10981 aagaggtgga gcaagataca acactagata tatacaaggt gttggtatag gaactataac
    11041 agatagttta acagcaataa aatataatgt atttgataag aaaaaattcg atatggatac
    11101 attgcttaaa gcacttgatg ctaattttga aggatatgaa gctatactta atttagtttc
    11161 aaataagact cctaaatatg gaaatgatga tgattatgct gatgaaataa tgcaagagat
    11221 attcaatgca tattacaatg aggtaactgg tagaccaact gtttgtggtg gagaatacag
    11281 agtagatatg ttaccaacaa cttgtcatat atactttgga gaaattatgg gagcaagtcc
    11341 aaatggtaga ctttgtgcaa aaccagtttc agagggaatt tcacctgaaa aaggtggaga
    11401 tactaatgga cctacagcag ttataaaatc ttgtgctaag atggaccaca taaaaactgg
    11461 tggaacatta ttaaatcaaa gatttgcacc atcagtagtt caaggagaaa aagggttaga
    11521 taatatggct aatctagtaa gagcatactt taatatggat ggtcatcata tacaatttaa
    11581 tgtatttgat aaaaatgtac tgttagaggc tcaaaagaat cctcaggatt ataaagactt
    11641 aatagttaga gttgcaggat atagtgacca tttcaataat ttaagtagaa cactacaaga
    11701 tgagataata ggaagaactg agcaaacatt ctaggtttgc ttgtaaataa aacttcttac
    11761 tatataaaat cgtaactgat ataataaagt ataaatggtt tgattccatt tttaaaattt
    11821 tagattaatt atgagctaac tttctaaata ggaagttagc tcgttaagtt taatgaaaaa
    11881 atcgttaaat ttatatttag taaatcatac acataattag taagtaatac atacaaaaga
    11941 ataatttatt atagttataa taaggttata ttttataaaa ataagataat tttaagcagt
    12001 taaaataaag tcaatttagc tactttaaaa taaaactaat agaaaaaagg agatttgata
    12061 tgaaaacttt aggatttatt ggttcaggga atatgggaag tgcaatgata ggtggcatag
    12121 tacagtcagg attagtaaag gcagaagata taactgtatc agatttaagc caagttgcct
    12181 tagataaagt aaaagaagaa tttggagtaa atactacaac tgattcaaag gtagctgtta
    12241 caaattctga tgtggtaata gtagcactta aacctaatat atgcgaaaag gctttaactc
    12301 cacttaaaga attaatagat gcaaataaaa taatagtatc tatagcagca ggtaaaacta
    12361 tagaaagttt ggaaagtttt ataggttcag ataaaaaaat agtaagagta atgcctaata
    12421 ctccagccct tgttggtgag ggaatgtcag ctctttgtaa aaatagtaat gtaacagatg
    12481 aagaattaaa tatgattaag gctctgtttg aatcttttgg tgaggcagaa atagtatcag
    12541 aatatttaat ggatacagtt acaggtatta gtggttcttc tcctgcatat gtatttatgt
    12601 ttatagaagc tatggcagat gcagcagtat tagctggaat gccaagacaa caagcttata
    12661 aatttgcatc tcaagcagta atggggtcag ctaagatggt tcttgaaact ggaaaacatc
    12721 caggtgaatt aaaagatatg gtttgttctc cagcaggaac tactatagaa gctgttaaag
    12781 tattagaaga agaaggtttt agagcagcag ttataaaatc tattgttgct tgcatagata
    12841 agtctaaaga tatgagcaaa taacagatgt aattaagttt actatatggt ttcttgaatt
    12901 gtttttttag tgcttattta tatctataca taaatgtatt tggtataagt aaaataagag
    12961 taatttacta taaaaaggaa tttaatgatt gagtgagaat ttattactt
//
LOCUS       DAELJO010000108        12984 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELJO010000108 DAELJO010000000
VERSION     DAELJO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.108"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            49..750
                     /locus_tag="KPB88_002463"
     CDS             49..750
                     /locus_tag="KPB88_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG0054732.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1021..1839
                     /gene="larE"
                     /locus_tag="KPB88_002464"
     CDS             1021..1839
                     /gene="larE"
                     /locus_tag="KPB88_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG0054733.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1894..3042
                     /locus_tag="KPB88_002465"
     CDS             1894..3042
                     /locus_tag="KPB88_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0054734.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3186..4574
                     /locus_tag="KPB88_002466"
     CDS             3186..4574
                     /locus_tag="KPB88_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0054735.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4603..5301
                     /locus_tag="KPB88_002467"
     CDS             4603..5301
                     /locus_tag="KPB88_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054736.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5304..6737
                     /locus_tag="KPB88_002468"
     CDS             5304..6737
                     /locus_tag="KPB88_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0054737.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6759..7496
                     /gene="truA"
                     /locus_tag="KPB88_002469"
     CDS             6759..7496
                     /gene="truA"
                     /locus_tag="KPB88_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0054738.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7607..7814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8067..9266
                     /locus_tag="KPB88_002470"
     CDS             8067..9266
                     /locus_tag="KPB88_002470"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG0054739.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9513..10559
                     /locus_tag="KPB88_002471"
     CDS             9513..10559
                     /locus_tag="KPB88_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG0054740.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIDKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10667..11830
                     /locus_tag="KPB88_002472"
     CDS             10667..11830
                     /locus_tag="KPB88_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0054741.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPTWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11919..12299)
                     /locus_tag="KPB88_002473"
     CDS             complement(11919..12299)
                     /locus_tag="KPB88_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG0054742.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12302..12961)
                     /locus_tag="KPB88_002474"
     CDS             complement(12302..12961)
                     /locus_tag="KPB88_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG0054743.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5221 a         1287 c         2258 g         4218 t
ORIGIN      
        1 aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg agataaatat gagtattgaa
       61 gatttatatg attttgaatg tgttcctttg aacaatgaga ttgatattga tgttttattt
      121 gaaaagatga tagaagatgg aaaagaaaaa gaatatacac caattatagt cattgatgaa
      181 aaagtagggc tattaagaaa aaatatagat atgattacta aagagtatgg ttctcttgaa
      241 aactatagag agtattgttt aacaaaatat aatgaaattg atgtaaatca attttttgat
      301 tttaggaaga gtgagatttc agaattatta aaggagctaa ttgatttaga agatgatttt
      361 tatgattata aacattttag accaattatg aatatagata tatttcatga atataacaaa
      421 gtttacatag ctaaaattcc aacaattaaa ccatatgaag tatttgcata catacctatt
      481 ggcggattta atgattgtcc tagagatgaa gaacatatag ctattgcaaa atattggtat
      541 gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata cagttcaata ttctgtaaaa
      601 aatttaacta aagatggtaa aaaatttgat gacttatgta ttgaattagt attttattgt
      661 gaggatatta ttatacaagg gtatgaaact ttaaaggctc ttaaggatgt attaaaacgc
      721 tctacaatag tgcttttttg gtgggattga tattctatca tttagatagt attaaattgt
      781 tagtcaccaa gaatccactt gctttagtta tggggagtgt gtcaaactag gtttttatac
      841 acagtgtagc atagtaatat gtttaaatta caaaggttaa aaaaatactt taatattttt
      901 aatattataa ttaatagatt taaaggtaat tataatttat aaaataaata ctttatatgt
      961 tataatcatt aagaatgtaa aaaaataata tatagtttta tagttcatat ttttccagat
     1021 atggaggtaa attttgtgaa ggaaaaagaa aaattagata aattaaaaaa aatgttactt
     1081 gaattaggca gtgttgtagt tgcttattct ggtggagtag atagtaattt tttgcttaag
     1141 gtcgcaaaag atacattagg tgaaaatgtt atagctgtga ctattcatgc aatgatgcat
     1201 tcaagtaggg aaattgaaga ggctaaacaa tatactcaaa attttggtgt taaacatatc
     1261 atattaaata tagaaaactt tgatttaaaa gaatttaaag aaaatggtgt tgatagatgt
     1321 tatcattgta aaaaatatat attctctaaa ataaaagagg tagcaaaaga acataatata
     1381 aagtacatag tagatggaac aaatatagat gatttaggag actatagacc tgggcttaag
     1441 gcattgagtg aattaggagt aattagtcca ttaaaagata gtagtttaaa aaaagaagaa
     1501 attaggtcgc tttcaaaaac attaggatta aatactttta ataaaccatc atttgcatgt
     1561 cttgcgagta ggatacctta tggagttgaa ataacagatg aaaatttaag aatagtagaa
     1621 aagagtgaag aatatctatc caatttaggt ttttctcaat ttagagtgag aatgcatgga
     1681 gatatagcta gaatagaagt tggacaggaa gaactaggta aattttttga aaataacaat
     1741 tttaataaag ttgatactaa attaaaaata tttggcttta aatatgttac actagatatg
     1801 tcaggatata aaatgggaag tatgaattta aatgtgtaga ttattttaaa aataaataag
     1861 ttttatagtt aatataagga aacaggaggt tttatgaaaa attatacgtt aatatcgcca
     1921 tgtttttttg gaatggaaaa aatgctggct agagagatta ctaatttagg atatgaaata
     1981 ataaaaacag aagatggaag aataacatat aaaactgatg agtttggaat agcaaagtca
     2041 aacatgtggt tgagatgtgc tgaaagagta catcttaaaa ttgctgaatt tgaggcaaaa
     2101 agctttgatg aattatttga aaatacaaaa agaataaact ggtctagata tataccttat
     2161 ggagctcaat ttcctatatc aaaagcttcc tctataaaat caaaattata cagtacacca
     2221 gatgtacaag ccatagtaaa aaaggctata gtagaaagct taaagaaaag ttatttggag
     2281 gatggtttgc ttaaagaaga taaggagaaa taccctattt ttgtatttat acacaaagac
     2341 aaagtaacca tttctataga tacaacagga gatgctttgc ataagagagg atatagagaa
     2401 aaggcaaata aagctccaat aagagagact ttagctgctg ggcttattta tttaactccc
     2461 tggaaagcag gtagagtttt agtagaccct atgtgtggtt ctggtactat attgattgaa
     2521 gctgctatga ttggtataaa tatggcacct ggcttaaata gagagtttat atcagaaaag
     2581 tggagaactc ttgataagaa aatatggtgg gatgtaagaa aagatgcttt taataagata
     2641 gataatgaat ctaaatttaa gatttatgga tatgatatag atgaagaatc tatagatata
     2701 gctagagaaa atgctgaaat agcaggtgta gatgaatata tagagtttaa tgtaggagat
     2761 gcaactcaat ttaaaagtga agatgagttt ggatttataa tcactaatcc tccttacgga
     2821 gaaagattag aagataaaga cagtgttaaa cagttgtata aggagcttgg ttatgcattt
     2881 agaaaattaa aaaattggtc gtattatttg ataacttctt atgaagactt tgaatatgag
     2941 tttgggcaaa aagcagacaa aaaaagaaaa ttgtataatg gtatgttaaa aactaacttt
     3001 ttccaatatc cgggtcctaa gcctcctaga aataataaat aagttttaca tgatttattt
     3061 gtaaaatata aaattttatt aaaataagta aaacattatg atgccttgaa acgtctaatt
     3121 attgtgagga gatttttgtg aacaaattta aagatttaaa gatttatagg attaggagga
     3181 gttaaatgaa agtgttaaag cttattactg tagtagccct gatgatgtta tttgttactg
     3241 gatgtaatat aaaaactgaa tctccagaac aattagcgaa gatacctgac tatgatgata
     3301 caaaaaaagt attgtatgat ggtattgacc agttactaaa accagattca tctatgatat
     3361 tgccttctaa tacaaaagaa gtaggaaaaa taaataaggt agatttaaac agcgatggaa
     3421 ttgatgaatt agtagtattt gaacaaaaag aagatttaag taataatgtg agtcaggtag
     3481 ggtttgtaac cttgagttac aatggagaaa aatacgtact tggagaccat tttttagaaa
     3541 atggcgaatc aatagaatat gctaattttt atgatttgga ctcagatgga tataaggaag
     3601 ttatcttatt ggttaaatcc aaagacaaaa cgaatatgca tatatataaa gtagtagata
     3661 atgaaataac aaaaatatat gacttggatg catcatggct tcaaaataaa gaagatttta
     3721 atgacatgaa agtaaaaatt ggatatatag atggagatga caagttagac atattgatgc
     3781 ttcatttgaa taataagact aatgaaatgt ttgcaagtgt tgcaaatttt gatgggaaaa
     3841 tgaaaatcaa agattctgtt aagtttgaaa atgtaaaaaa cttaagtgaa ttatatataa
     3901 cattaggtaa tgttgcttca tctgttggaa attcttctgc agctataaaa ggtattgtac
     3961 ttgatattcc tatattgaaa gataataact atattacgca gatactttat atgaaagatg
     4021 ggaaaattaa caaagctttt agtgattatg ataaaactat tactaaatct tattatatac
     4081 ctgtagatga tattgacaaa gataagatta tagaaatacc tatagttgca ggcagtacag
     4141 gtaataataa aaatacttat agttctaaat catctgcaac tataagttgg tatagatgga
     4201 atggaaaaga aggtgctgac tcaagtttaa tatttacaag tcagatatat tataattata
     4261 aatataattt taaattattt attccaaata atttatttga taaaatactt gttgaacagg
     4321 aatttgttgg agaaaaagca gtatttaaat tttactattt tgactattca gaccgtaaga
     4381 atttatttaa tatagtagta gagagtaaaa ataaacttga agatagaaaa aatacaggta
     4441 ctcaaaattc tattgtactt caagagagcg atgaatatac tttcttacta gttgtaaaca
     4501 atgcaaatga aatggataag ctagatatga ctatagacgc attaaaagaa tatttctcat
     4561 taatatatgg ttaatattat actcaattgg aggttttatt ttatgaagga aaagatactt
     4621 atactagaag atgaaattgg tattagaagt tttgttagta taaacttaaa aagggaaggc
     4681 tatgaaatag ttgaggcagg tactggaaga gaagctatag aaaaaatgac aacagaaaag
     4741 gatattacaa tagctctttt agatgtaatg cttccagata taagtggtat agaagtatgt
     4801 aaatttataa gggagaattt tgaccaagta ggtataataa tgcttactgc taaagctcaa
     4861 gaagatgata aaatagaagg atttatctca ggagcagatg attacatcat aaaaccattt
     4921 agtataaaag agcttttagt cagagtttct gctcttctta gaagagtagc aaaggatgat
     4981 tcaagtgtta agtcgagtga gatagtttct ccaccattta tacttgatat agacaaaaga
     5041 aaattattta aaaatggaaa ggaaattgag cttacaccaa ctgaattttc aatagtgaaa
     5101 tatctaatat caaatgctaa gcagtcgtta agtagagacc agatattaga cgaagtatgg
     5161 ggaactaact atttatatga ttttaaaata gtagatgtaa atataagaag gataagaaat
     5221 aaaatagaag atgacccatc aaaaccaaaa tatatacaaa ctatttgggg ttatggatac
     5281 tgttttagaa aggaagaata atagtgttac attttgaagg tctgagaaaa aaagtaataa
     5341 aaaactattt tataataatt attataatgg ttactctgtt tgaaggattg ttcatgtttt
     5401 acatacaaaa ctactattat gattctgtca aacagctact tgaatctgaa ataaaatatg
     5461 ctgatgaata taatgctatt acaatggaaa ctacaagttt tgagaaaaaa gtaaaaaata
     5521 tatttgataa acaaccctta actaaaaatt cagaatttgg aatttcaata atagataaag
     5581 ataaaaatat tatattagac caatatggtt ttaaaagtaa agaaaaagca aactatgaag
     5641 atgttaacaa tgcattaaaa gatataaaaa ctaaaaattt aacaccttat acttatagaa
     5701 taccagatac aggggagcat gttatgagta tctccttacc acttaaggta aataatataa
     5761 tagaaggtgt tgttagatat acagtatcat tagatgctat tgataatgca atattaaagc
     5821 aagctacatg gcttatatta gcaggaatat ttatacttat tatagctata ctaataagtc
     5881 ttaaatttgc tgagactctt ataaaaccat tacgagagct aaagaaattt gcaaatgaat
     5941 tggctgtagg aaattataat ataaaattag aaaagatgaa gattgtagat gatgaaattg
     6001 gtgatttagc acagacattt gaacacatgg cacatgagat tgacaaaagt gaaaaattaa
     6061 aagaggaatt tatatcatct gtatctcatg aactaaggac tcctcttaca tctataaagg
     6121 ggtggagtga aaccttaggt tatgaaagta taacaagaga agaactagac ttaggtttag
     6181 gcattataca agatgaaaca gaaagactta taaaactagt tgaagaatta ttagactttt
     6241 caaggctttc ttcagacagg ataaaacttc atgttgatat agttgatgtt gaaggtttaa
     6301 ttgtaggtgt tgtaaatcaa cttaaagtta aagcagctga aaaagatata tcacttttat
     6361 ttgaatttga aaatgaattt atagagaaca ttcaaggaga taaaaataga ttaagacagg
     6421 ttctcataaa tttaatacaa aattcattta aatttacgag tcaaggtggt tacataaaag
     6481 ttgtagcttc tcaagatgaa gagataacaa ccatatcagt agaggataat ggttcaggta
     6541 tagaaaagca aaatttaaat aaagtgttag ataaattttt ccaagaagat tataataagg
     6601 ctggtagtgg attaggacta gcaattagta atgaaattgt aaaacttcat ggtggaagaa
     6661 tgaaaataga aagtgaaaag aatgttggaa caaaaataac ttttaatata aaaaataaat
     6721 ttgctaaaca agcataaatt cttagaaaga ggattaagat gagaaacata aaaatgatag
     6781 tagcatacga tggttcaaga tataaaggat atcaaaaatt aggtgacaat aatatgacta
     6841 ttcaagaaaa acttgaaaat gttttaagta aaatgactaa tgaaacagtg gagataatag
     6901 gttctggaag aactgatatg ggagctcatg caagaggaca agtggtaaat tttagaacta
     6961 attgtatgga ttctttggat aaaattcaaa aatatttata tgaatattta ccagaagata
     7021 tagttgtaaa aactgttgaa gaagttgatg aaagatttca ttcaagatac aatgtaaaat
     7081 caaagactta tatgtataaa attgacaata ataagtatca taatcctttt ataagaaaat
     7141 atgctactca tgtaagtaaa aaattagatt tagatagaat gagaaaagca agtgagtatt
     7201 tagtaggaga acatgatttt actagttttg cttcttctaa atctaagaaa aaatcaaatg
     7261 taagagaaat ttattctata aatataaaag aagatgataa tgttatagaa atatatgtag
     7321 aaggaaatgg atttttgtat aacatggtaa ggataatagt aggtgctttg atagatgttg
     7381 gtctaaaaag aagagctcca caagatatta agtatatgct tgaatcaaaa gatagatgtc
     7441 aatcatcaga tactgcacca gctaaaggac tatgtctttg gaaagtaaca tattagtcaa
     7501 ttatataaaa ctctctttaa aataaaaata aaataaatta tatagttgac ataaatggaa
     7561 aaataaacta ttatatattt tataagagag atataaaact ttatatttaa atcctgtgag
     7621 aaagagagta acttctatgt gttttataga gagctgagga tggtggaagc ttagcaaata
     7681 agtattagtg aaaagaactt tggaggatac taattgaatt tagtatgtta gtaggtaatc
     7741 tcgccttaag agaaagagtg gataagttta tcttatcaat tagagtggta acgcggatat
     7801 atttcgtctc ttggtttttc caagaggcgt tttttaatat atttaattat tagtgtttat
     7861 ctggtatgtt gaacatttac taattgatta gctaagttta tcaataagag tggtaccgcg
     7921 gatatttcgc ctcttagtat tttaaaatac taaggggttt ttttagtatt aaaatgattt
     7981 tatgtgagtc taaattctaa gagtcattca atattaagaa tttctataaa ataaaaatta
     8041 taatttaaga ttgggaggaa ttaaaaatga aggaaaaagt agtattagca tattcaggag
     8101 gacttgacac atcaatcata ataccttggt taaaggaaaa ttatgaagat atagatgtta
     8161 tagctgtttg tggaaatgta ggtcaggaag ataaaatgga agatgtatat gaaaaagctt
     8221 tacaaagtgg agcatcaaag gcatatgtgg atgatataag tgaagaattt gtaactgaaa
     8281 ctatatttaa agccgtaaaa gcagaggcta aatatgaagg aaaatactta ttaggaactt
     8341 ctttagcaag acctataata gctaaaaaat tagttgaagt tgctcataaa gaaggtgcaa
     8401 aatatatatg ccatggatgt acaggaaaag gtaatgacca agttagattt gaagctacaa
     8461 ttgcagctct tgacccgact ataaaagtta ttgcaccttg gagaatttgg gatataaaat
     8521 caagagaaga tgctatagac tatgctgaaa aacataatat aaaggtaaca gcaactaaag
     8581 ctaaaatata ttcagtagat gctaaccttt ggcatgtttc aacagaaggt ggagatatag
     8641 agcacctaga aaatgaacat aaaaaagatg tttataaaca atgtgtagac ccagaagatg
     8701 catgtgatgt agctgaatat gtagaagttt actttgaaaa aggtgtacct aaaaaggtaa
     8761 atggtgaaga attatctcca gttgcactga ttcataagtt aaatgagttg ggttgtaaac
     8821 atggaattgg tgttatagat atagttgaga atagacttgt tggtatgaaa tcaagaggta
     8881 tatatgagac tccaggagga acaatccttt atgaagcaca taatatttta gaaagtgcta
     8941 ctttagacaa agatacatta catttcaaac aaatggtatc ttataaatac ggagaactta
     9001 tttacaatgg actatggtat tgtaaattaa gagaatctat agatgcattt atggaacaaa
     9061 ctcaagataa tgttacagga acagtaaaag ttaaacttta caaaggaaat ataaaacctg
     9121 ctggaatatt tactgaaaat gctctttatg atgaaggaat atcttctttt ggaaatagtg
     9181 aactttatga ccacaaagat gcagaaggat ttattaattt attcacttta ccattaaaaa
     9241 tcagagctat gaaagctgga aaataataaa ataaattatt taagagtgta taaaaactat
     9301 ttttaggttt ttatatgctc tttttttatt tgaatttatt aacttataca gtaaatagta
     9361 taaaaaaata tattatagtt ccaaatggct taataatgtg aaaatataag acctaaaata
     9421 taaattttat aaatacaata gttattgact ctctcattgt aagagggttt atactgaatc
     9481 taatgaaaat ttatagtgag gagatagatt atatgagaaa aataattgaa attaaacatc
     9541 attcacatga ttatgagtgt atgtggaatg gtatagaaga tttatatata aataaaaccg
     9601 gtgaatgcct tccaaacgat tttttctttt tactgtcagg ttttggttca ttttgttata
     9661 tgaaaacaaa taaagctgat ttgaaaagaa tggtagcttt gggagatgga aggacaaaac
     9721 agatgtataa atttttagca ccaattgttg gatttgaata caaacatcat gagtttaaaa
     9781 agtatgagca agcattaaag aaagcaaaat ctgaaattga tgatggatat ccagttattt
     9841 taggagcttt agatatgtat tatctaccat attataagaa gctatatcat aaagaacata
     9901 ttccttttca ttatatatta atggtaggat atgatgatga aggaatatat ctgtatgatt
     9961 gtggtagaac agaattatta catctttctt atgataaatt acgtgaaagc atgaattgca
    10021 attcccctgg attaagcaaa gaaaatacta tttgcacaat aagaatgaat acttcaaaga
    10081 ataaatataa cattgctaga gaagctattg ctataaagcg tgatatgttt ttaaatccac
    10141 ctaaaggatt tttgggatat aaaggttttg aaaagtttat taaagagttg ccacagtgga
    10201 acactgaatt ggataaagat gattatgata agattttgac caatatggtt atgttttttg
    10261 gaacagtacc aacaattcca aatgcaatca aaggaattaa agaaccagat actgttcaat
    10321 ttaaaggtgg atttgataaa atgagtagaa tgctcaacag tattggggag gaatacaaaa
    10381 acgactcttt tatacaagta gctgatattt ttgataaaac tgctattata attgataaaa
    10441 tatctaatat tataatagat tatttaactc aaacatgtga tgatacagaa caacttccgt
    10501 tattgttctc tgaggtctta gagcatatga aaacaggata tttaatcttg gatgtataaa
    10561 aaatatattg ttgaccctaa cattacgtaa tagtgtaata tgaaaatata gcttgaatat
    10621 attagtaggt attgatggct gtaaaattaa aatttgaggt gtggatatga gaacagtgaa
    10681 acaagtttct gatttaactg gaataagtgt gagggcatta cattattatg atgaaatagg
    10741 tcttttaaaa ccaaataaaa ttacagatgc aggatataga ctttatgatg atgaatctat
    10801 taaaactctt caacaaattt tattttttaa ggagattgat attcctttaa gagaggttaa
    10861 ggaaataatg tcaagccaat attttgataa ggttgaagct ctaaaaaatc aaaaaaaatt
    10921 acttatttta aaacgaaaga gactggatga attgatagag cttataaatc aaactctaag
    10981 aggagaagga aatattgatt ttaaagagtt tgatatgagc gagtattttg atgttttaga
    11041 agaatttaaa agagagcata ggaataaggt aattaaaatt tatggtagtg tagaaaaata
    11101 caatgaatat atagaaagag ttaagtctaa tgaagagaaa attgctaaaa tggctataaa
    11161 acaatatgga actattgaga agtttgccca agctataaaa acaaatttta gcagtgatat
    11221 attgaattta ggagaaaaat ttgatagata taaaaatgat tgtttaaaag aaaaacatcc
    11281 aaaattaaaa gaattatata gaaagcttgt agaagattta agtaggaatc cttcttcaac
    11341 agaccttcaa gaaatagcaa aagcgataac agatatatct aaaaaagatt atgaaatttt
    11401 ctctatggat actggtgatg acaactggta ctatatggtt caaaattatc tagtaaatcc
    11461 tacgtggata gaagaagtag ataaaaagta tggaagtgga gcaggtaagt ttattggaca
    11521 agtactaaag acttatctaa gagatagaaa accaaagata aatacattgt atgaaaaact
    11581 tgtagaagat ttgagtagag attgttcttc aagggaagtt caaagcattg ttgaagaaat
    11641 agacaatgaa atgaaaagaa gtaatgaatt ttataaaata gacaatggtg aaagatactt
    11701 tgattatatg tcagagcttt atttacaaga ttctaattat ataaaagtaa ctgataagaa
    11761 ttatggtgat ggtgcatcta agtttattgg agaagctttt aagatttatt ttgataataa
    11821 taattgttga aaatttggaa gtaaaaaata tgtaagtttt ataaaagcac aaaaataagg
    11881 atgtagatta gctccatcta tatccttatt taaagttatt aaaatatata gtttacatca
    11941 aacttaacac tttcaataat atttcccaaa tatgttttta tactttcaac ttcttctaaa
    12001 gattttaatt tattttcagt ataaattgct ttgtccagaa tattaaacct taatcctatt
    12061 tcatcaaagt actcatgatt taaaactttg taccaatggt ttttttcttt attccaactc
    12121 gtagatattg aatcagcttc tttttgagct ttttcccaat cttcattttc tatagatttt
    12181 tctaaaacag atatattgtc tttatatcct tctgtaaaat catatagttt attgttaaca
    12241 taaaatccaa ataatacaaa taagatagtc catagtataa caaatgttgt agatttcaat
    12301 attaatcacc ctttttattt tgaataaata ttttgtcatt ttcatctaaa acacaaataa
    12361 gaacatcttt aaaagtttct atgtgattag attttaaaat acccattaac cagttagtat
    12421 ctttttgagc tatgtctaga tttttattta ttattctacc atccagtata agtgggatag
    12481 gtaaatgatt aaaagctctt ggaggagtac tattgtagct tgatgctggt acaacactta
    12541 gattaccatc agtctccaaa atagcatatt gtacatcttt taggttaaag tatccttgaa
    12601 tcctaagttg ttctaataat tcatctatac taattctttc ttttttaagc tctttatata
    12661 caatttttcc tttatctatt aatacagaag gtttacctga taaaaatgaa cttaattttc
    12721 tactttttaa tgataagaat gaaattagag tctgcatgat tacaagtcca gatattgctg
    12781 caacaccatt tatgattggt atattattat tttccattgg gacagatgca acttctgcaa
    12841 ttaatagtgt tataactaaa tcaaaacagt tcatttcagc tatttcacct tttcccatga
    12901 ctctaagtgc aattagcact gttatgtata atataatact tcttattaat actactgtca
    12961 tatctatcaa ttactccttt aaaa
//
LOCUS       DAELJO010000109        12921 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELJO010000109 DAELJO010000000
VERSION     DAELJO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.109"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..138
                     /locus_tag="KPB88_002475"
     CDS             <1..138
                     /locus_tag="KPB88_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0054744.1"
                     /translation="HRTKDGKGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFD
                     A"
     gene            131..571
                     /locus_tag="KPB88_002476"
     CDS             131..571
                     /locus_tag="KPB88_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054745.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRILKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYINVNEDLVRGTNMN
                     YEIIDNVLHFFVDYNYFTMKSNSTDKMDTVELFGGLKRGDSIEN"
     gene            555..743
                     /locus_tag="KPB88_002477"
     CDS             555..743
                     /locus_tag="KPB88_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054746.1"
                     /translation="MVLKTNDLDKKSNDYKFTKEQIVNSKKYVNRKDLLNAILKEDEL
                     YSFSEVEDMINKFMKGVS"
     gene            744..2054
                     /locus_tag="KPB88_002478"
     CDS             744..2054
                     /locus_tag="KPB88_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG0054747.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPIEL
                     DWGINEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGIVSIENKIKDKDLVESSLIYWVTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNDKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            2071..2541
                     /locus_tag="KPB88_002479"
     CDS             2071..2541
                     /locus_tag="KPB88_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0054748.1"
                     /translation="MAQTINAKDTVSAKKAECFVTIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDMQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            2611..3051
                     /locus_tag="KPB88_002480"
     CDS             2611..3051
                     /locus_tag="KPB88_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0054749.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASDRFVDENGKPVEWELKVLSSE
                     EDESLRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTTFEDKVEEAKN"
     gene            3394..3555
                     /locus_tag="KPB88_002481"
     CDS             3394..3555
                     /locus_tag="KPB88_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054750.1"
                     /translation="MDNFLIGVLASLTASLIGYIVCLCIKKVKSHSVQESDLDVELKF
                     SFKFKKNKH"
     repeat_region   3837..4591
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3837..3865
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4725..5249
                     /locus_tag="KPB88_002482"
     CDS             4725..5249
                     /locus_tag="KPB88_002482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054751.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            5427..5615
                     /locus_tag="KPB88_002483"
     CDS             5427..5615
                     /locus_tag="KPB88_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054752.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            5734..6603
                     /locus_tag="KPB88_002484"
     CDS             5734..6603
                     /locus_tag="KPB88_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBG0054753.1"
                     /translation="MENLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QMAVCEIIKKIDLEDSEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIQG
                     EKKNFHVVYKEAM"
     gene            6603..6752
                     /locus_tag="KPB88_002485"
     CDS             6603..6752
                     /locus_tag="KPB88_002485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054754.1"
                     /translation="MGDNLLNNVCNDEKEYLISYLKTLRQKDEHDFYVFKQIVDKYCK
                     QAKYK"
     gene            6882..7658
                     /locus_tag="KPB88_002486"
     CDS             6882..7658
                     /locus_tag="KPB88_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG0054755.1"
                     /translation="MGLFRKSKEPCCICGKEKTDQKIKDGAVCIECLMDYMEYKDKQF
                     TFKDGLPTKSEIQIVLDSKKQNDFLVEKFNVTKEINSFIKFDETNKLIYIETKRKNGK
                     IKKNVYSFENITGFELLEDGETITKGGLGSAIAGGVLFGGTGAIVGSIVGKKKTKKII
                     ENLQIKLTLKTISEPVAYINLINVKTKTNSIGYERAYAEAQEILSILAIILKDIEKKR
                     EEINNNNSNADEILKYKNLLDLGAITEEEFNTKKKELLNL"
     gene            7721..10072
                     /locus_tag="KPB88_002487"
     CDS             7721..10072
                     /locus_tag="KPB88_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG0054756.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADGQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQQSDGGLNKMIFQSAERSRASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRSMASEGMLTADIVKNSLLSAAEQTNAEFE
                     KMPYTLGQIFTSVKNNAVMIFGAIQKKIEDTVSSKGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGSSLVVQGLGWIITQALNVANVFAQNWSIIAPVIWG
                     IIAVIAIYKITIISLLAIQTLHTTLTLAQSFATALLNGELMAETRFLLLNKLETLGLS
                     QANALLCVSILKVVAVMALVIAAIFVGVAIFNHFAGTSISATGVVVGCFYFLGACIYD
                     VFAAAWNIIMAFAEFFVNSFNIVIYNVQLLFYKFQNFVIDSMGDVGGSFDNCATALAN
                     AFVSAANIAIKGINGVIKTLNLIPGVNIKTFGSLDKIDSFVKQYKDYQKTLKEPVKPA
                     EWKAPTMKLKNPVDSFKKGYEVGQNLENKLKDAFDINKIAEKAKKDLRLDDLWDKKYG
                     LGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFT
                     GVNIKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            10089..10784
                     /locus_tag="KPB88_002488"
     CDS             10089..10784
                     /locus_tag="KPB88_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0054757.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVIAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLKLG"
     gene            10777..12669
                     /locus_tag="KPB88_002489"
     CDS             10777..12669
                     /locus_tag="KPB88_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0054758.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSNNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            12683..>12921
                     /locus_tag="KPB88_002490"
     CDS             12683..>12921
                     /locus_tag="KPB88_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0054759.1"
                     /translation="MSQELLQIIKKTAIDAVETSNPMQIAFGTIESLNPLVVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQ"
BASE COUNT         5163 a         1352 c         2489 g         3917 t
ORIGIN      
        1 catagaacta aagatggtaa gggatgggtt aaaggacaac atttcttaac tatttcagaa
       61 atggagttac aaagccaagt tgataagatt atagagaaaa agttattaat tttgttgaaa
      121 ggagtgtttg atgcttaata atattataga tggaatatct attaaattag ataaatcatt
      181 tggaaatgaa tatacaattt acagtgaaga tgtagagcaa ggtataaatg aaccttgttt
      241 ttttattgtt cctttaaatc caagcaaagt atcatatcca agtggcagga tattaaaaaa
      301 gaactctttt gatgtacatt attttcctaa aagtaatgat aaatcatttg aaataaatga
      361 ggtagctgag atgctactgg aggaattaga gtatataaac gtgaacgaag atttagttag
      421 aggcacaaat atgaactatg aaattataga taatgtactt catttcttcg ttgattacaa
      481 ttatttcact atgaaaagta atagtacaga taagatggat acagtagagt tatttggtgg
      541 attaaagaga ggtgatagta ttgaaaacta atgatttaga taaaaaatct aatgactata
      601 agtttactaa agagcagata gttaattcta agaagtatgt aaatagaaaa gacttgttaa
      661 atgcaatttt aaaagaagat gaattgtatt ccttctcaga ggtagaggat atgataaata
      721 aatttatgaa aggagtgagt tagatggctt taggtggagg aacatttgta acacagaata
      781 aaatacttcc aggtgcttat ataaatttta tctcagctaa gagggcaact agttcattat
      841 ctgatagagg tattgtagca atgccaattg agcttgattg gggtattaat gaagaagtat
      901 ttcaagtaac cagtgatgat tttgagaagt attcagtaaa gtattttggg tatgattata
      961 ctcatgagaa gctgaaaggt ttgagagatt tattcaaaaa tataaggttg ggatattttt
     1021 ataaattaaa taaaggtgtt aaagccagtt gtactatagc cacagcaaaa tatagtggta
     1081 tcagaggaaa tgacttaaaa gtaacagtta caacaaatat agatgataat gctaagtttg
     1141 atgttgtaac acttttagat aataagaagg tagatactca aatagcaaag gttattacag
     1201 acttacaaga caatgactat atcacttgga agaaggatgc aacactagaa gcaagtgcag
     1261 gacttgtatt tactggtgga actaatggcg aagctgtgac aggagcagag taccaagctt
     1321 tcttggataa aatagaaagc tatagcttta atgcactagg gtgtttggct acaacaacag
     1381 agattaaaag tttatttgta gaatttacaa agagaatgag agataaggta ggagctaagt
     1441 tccaaacagt actatataag aaaagtgatg cagattatga aggtatagta tctatagaaa
     1501 ataagattaa agataaagat ttagttgaat ctagcttgat ttattgggta actggagcta
     1561 tagcaggatg cgatataaat aaatctaata ctaataaaaa gtatgatggt gagtttgatg
     1621 ttgatgtaaa ttacactcaa atacaacttg aagaagcact aaagagtggt aaatttatat
     1681 ttcataaagt tggtgatgaa gttcatgtgt tagaggacat aaatactttt gtatcattta
     1741 cagatgataa aaatgacgac ttttcaagta atcaaagtgt tagagtatta gaccaaattg
     1801 ctaatgacat agcgacttta tttaatgata aatatcttgg taaagtgcca aatgataaag
     1861 ctggtcgtat ttcattttgg aatgatgttg ttaagcatca caaagaatta gaaaatataa
     1921 gggcaataga agattttaaa actgatgatg ttagtgtaga gcttggaaat gacaagaaaa
     1981 cagttatagt aagtgatgcg gttaaggtta taaatgctat gagtaaactt tatatgactg
     2041 tttcagttag ttagggagga gagtgaaaat atggctcaaa caataaacgc taaagacaca
     2101 gtcagtgcaa aaaaagctga gtgttttgta actatagaag gcaaaagata taattttatg
     2161 caagctatag atttagaagc taaaatggag aaaaataaaa gtgaggttcc aattttagga
     2221 agaacaacaa agggaaacaa aacaactggt agtacaaata ctggaagtgc aacatttcat
     2281 tataatactt ctatttttag agagttactt tatagatata aagaaactgg tgaggatatt
     2341 tactttgata tgcaagttac caatgaagac cctacatctg ctgtaggaag acagacagta
     2401 gtacttaaag attgtaatat ggacagtgga ataattacta aatttgatgc tgatggtgaa
     2461 tatttagatg aagacatgga ctttactttt gaggattggg agctagttga aaagtttaat
     2521 ttattagcag gaatggagta aaatacacat ttataaatta tagatgtgta ttttttatga
     2581 gaaaaattaa aataaaagga gaattaagaa atgagtaatt taagtgcttt tttaagtcaa
     2641 aatgcaataa aggttgataa tgtaaaatat gtggcaagtg atagattcgt agatgagaat
     2701 ggaaaaccag tagaatggga acttaaagtt ttatcatctg aagaagatga atcattaaga
     2761 agaaaatgca ctaaaagagt gaaagttata ggcaacaatg gtaaacatac aggtcaatat
     2821 acaagtgaaa ttgattataa cagttatgta gctgaattat gtgtagcatc tacagtattt
     2881 ccagatttaa aggatgccga actccaaaat agttatggag tgatgggaga agctcagtta
     2941 ttaaagacaa tgcttacagc aggtgagtat gtcaactata cagtaaaagt taatgaggtt
     3001 aatggatttg atacaacatt tgaagataaa gtagaagaag caaaaaacta attaggggcg
     3061 gcgattttga tgctagtatc acgcattatt gtatccaaaa attaaagtgg aaaccaggtg
     3121 attatatggg attagagatt aatgagagag cattagcagc cgcctcaata cttattaaga
     3181 tagaggatga agaggaagca atgaaagaag ctgaaagaga gagaaagagg ggtagaagaa
     3241 gatagcaaaa taaaaaaata aatatagaat aggtaaaata tgtaataatt atatgttata
     3301 atatttttag caagaagatg taatctacaa tttatagagt ggagttcata caaaagatta
     3361 tcctcccaac gtatagaagg gaggtgaata tgtatggata attttttgat tggtgtatta
     3421 gctagtttaa cagctagtct aatcggttac atagtttgtc tatgtatcaa aaaagtaaaa
     3481 agccactctg tgcaagagag tgacttagat gttgagctta aattttcatt taagttcaaa
     3541 aaaaataagc attaaattgt ttagaacttc actctacttc caaatagatt gtagttcttc
     3601 ttgtttttat tataccacaa attggtacag atattcaaaa ataatatatt tatgatataa
     3661 taaaaatgta gagattttgc agtgttcgat ttttgtaata aaatatggct taacaattgg
     3721 aatacaaggc attgagggtg tgtgataaat gttatcaatt gcactactca tggttcactg
     3781 caaatttgag agaggtgtgt atgtgtaggt attggaaatg ctaagtttat tttggggttt
     3841 tagattaact atatggaatg taaatgggac agactcaacc ctactattta caagctttat
     3901 aagttttaga ttaactatat ggaatgtaaa taatatattc tattattaaa agtaattgta
     3961 tcgggtacgt tttagattaa ctatatggaa tgtaaatttt atcaaataca catacatttt
     4021 aatattttct tcatgtttta gattaactat atggaatgta aattctaaaa ggtcacatat
     4081 atttattaaa tctgttgcct tgttttagat taactatatg gaatgtaaat cagctttaca
     4141 tatctatcta tgatttttga atctccatgt tttagattaa ctatatggaa tgtaaattct
     4201 aatttagttg ttaaaataaa ttccttacca attgttttag attaactata tggaatgtaa
     4261 attgagctgt tagataatat cctagcattt ttatttttcg ttttagatta actatatgga
     4321 atgtaaatta tgtacctaga aatttagaaa gttttggaga aatagtttta gattaactat
     4381 atggaatgta aatttcgcaa cttatgatgg tgaaatgatt acattaacag gttttatatt
     4441 aactatgtgg tatgtaaaga ttactctaaa agtcttttta tcattttcta cctcaatttt
     4501 atattaacta tgtggtatgt aaataaagca aaagatagga atgataattt taaaactcca
     4561 aacttttata ttaacaatgt ggagaaaaac taaatagaag aaagaagcac ttatttttgg
     4621 taggtgcttt tattttgctc aaaatatttt aaatcaatag actaagttct tatttttaga
     4681 tagaattata tttgaataaa gaatttaata ggggagagtt cattatgtgg ggaaaattta
     4741 aaaaattaag tttgttgaag aaaattttag tgatattttt aatatacttt gttgtattta
     4801 cagtatcaat gatgattcat caagcgatac gagattcaaa aaatagagat gaagtaaatg
     4861 aaggaaatat tacaaaagaa aatataatta gcgaaagaga aaaagaagat atttataaac
     4921 aagagatgca agcaaaagta gactctatga taccagaaga cttaaaagat aaaacaacat
     4981 attatgttaa catattaaac ccaacaaaag gtgagggata tatagttagt attcaagtgg
     5041 aaaattctag gtttaatgat gaaaatgagt gtagaaactt tactaaagaa tttgtaaaca
     5101 atataaaaga tatgaatgat atccattcag tgagaataag ctttattgtt gatgtgacac
     5161 tcacttataa tgtattttta gatgattgga ataatataaa aaataatgta aatttaattg
     5221 atgatttgga tttctcgtct ggaaattagg tgaatacttt attttaaaat taaatctgtt
     5281 atatagaaag cacttacttt ttggtaggtg cttttgtttt gctcaaaatt ggtcggttga
     5341 gtaaaataat tagaaaaaat tagtaaaaac tcttgaaaag tgtcgcgata caatgtataa
     5401 ttatattatc gcgatacaga aaagaggtga aaattattac tgatagcagt agagcagatt
     5461 acttcaagca gagacgacag aataagaaaa cttttagtgt tctactagat agagagaaag
     5521 tagaaaaaat tgaagaacat ttaaaaaagc agaacaagac taaaactatt tggcttgaag
     5581 aaaagattaa tgaagagtta gaaaaagagg aataaaaaat aagagacgtt ctccccgacc
     5641 aaagattgaa catcccttat tgacgtatat tatatacact aactatagta tacgtcattc
     5701 cttaaaaaaa ttcaattaag gagtgtaata gttatggaaa atttaatagt aaaagagttt
     5761 aatggaagtc aaatttatac ttttatgtgg aaagaaaaat cttgttggat agctaatcaa
     5821 atagttggat tattcgatta tgctgatgta tctaaaacaa tacaggattg cataaaagca
     5881 gaagactttg agattgaaca agagtatgat gtattgaaag gaaatgaatt taatgatttt
     5941 gtaactactt taaatgtagt cgcaaataat ataattagta ataaagctag aagtataact
     6001 attttttatg aagatggttt gtatggattt ttacaataca cagataaacc aattggtgta
     6061 cagtttagaa aatggcttag acgagaagtt ttaccaagca taagacaaac tggtgcatac
     6121 ataactaaca atgctaatcc cgaaaaacta agagaaaaag caagtgagat tgagaaatta
     6181 cagttggctt ataacagtac atctatgcta aaagagttat tagatggtgc aggctttgac
     6241 aataaatcca aactattaac agctaaaaca ttatataaga aagcaggaat tgatttacca
     6301 atagagataa acgaagaaga acattatttt gatacaaagc aaatagcatc taaactgaaa
     6361 atatattcta agagtaataa accagctcag atggctgttt gtgagattat taaaaagatt
     6421 gatttagaag atagtgaagt taagggagtt tgggaaacta atggaagttg gactggcact
     6481 gtaaataaat atacaaagag tgtaatagat aaggtcagaa attggataga ggaaaataat
     6541 agacctgcta agatacaagg tgagaagaag aatttccatg ttgtatataa ggaggcaatg
     6601 taatgggaga taatttacta aataatgtat gtaatgatga aaaagaatat cttatttctt
     6661 acctaaaaac tctaagacaa aaagatgaac atgattttta tgttttcaaa caaatagtag
     6721 ataaatattg taaacaagca aagtataaat aagaaattaa ttatataaat taaaaacact
     6781 tacttaggta ggtgtttttt tattgaaagg aagtgattat aatgtaaaaa ttttactaat
     6841 atagtataat aatcttataa aattatgtag ggggtaatat tatggggtta tttagaaaat
     6901 caaaagaacc atgctgtata tgtggaaaag aaaagacaga ccaaaaaata aaggatggtg
     6961 ctgtttgtat agaatgtcta atggattata tggagtataa agacaaacaa tttactttta
     7021 aagatggatt acctacaaaa tctgaaattc aaatcgttct agattctaag aaacagaatg
     7081 attttttagt tgaaaaattt aatgtaacta aggaaattaa cagttttata aagtttgatg
     7141 aaactaataa actcatatat atagagacta aaagaaaaaa tgggaaaata aagaaaaatg
     7201 tttatagttt tgaaaatata acaggctttg aattgttaga agacggagag actataacaa
     7261 aaggcggtct tgggagtgct atagcaggag gcgttttatt tggtggtaca ggagctattg
     7321 ttggtagcat tgtaggtaag aaaaagacaa aaaagataat cgaaaattta caaataaagc
     7381 ttacattaaa aactatttca gagcctgtgg catatataaa tttaattaat gtaaaaacaa
     7441 agacaaattc tattggatat gaaagagcgt atgcagaagc gcaagagatt ttatctattt
     7501 tagctataat tttaaaagat atagaaaaga aaagagaaga aattaataat aataactcta
     7561 atgcagatga aatattaaaa tataagaatt tattagactt aggagcaata acagaagaag
     7621 aatttaatac taagaaaaaa gaattattaa atttataata actagacact tacaaaagta
     7681 agtgtttttt tatggaaatt tatgaaagga gagtgataaa atggcaacaa tacaaacatc
     7741 tattcgaatt ttcgatggaa tgacacctgc ttttagacac atgactaatg ctatgaatat
     7801 tgtattaagt tcattcgagc aattacaaag aacatctagc aatgctatag atgctaatag
     7861 tattagaaca gctagagaag aactagcacg tgcagaagct gggtttgata gattagaaca
     7921 acaaataaga gaagctgatg ggcaacagcg aagacttaat gaggatataa ataagggtgc
     7981 aagttctaca gatagattag ttggaagtgc aaagaagtta gcagcaactt atttaggtat
     8041 aagaacatta ggaggtctag gaaatttaag cgaccagatg acaagtacta atgcgagact
     8101 gagtatgatt aacgatgggc aacaatcgga tggcggactt aacaaaatga tatttcaatc
     8161 agctgaaagg tcaagagcat cttatttaga tactgcaaaa atagtttcgc gtgtaggtat
     8221 gaatgcaggt aaagcattta gcagtacaaa agaaattgta ggttttgcag agcaattaaa
     8281 caaaaaattc gtaatagcag gtgcaagtac tgaggaaatg aattcggcat tgttacaact
     8341 aacacaagga ttaggaagtg gtgtgcttag aggtgaggaa ctaaatgctg tatttgagtc
     8401 agcacctaac attatccaat cgattgccga ttatttagac gtggatatag gaaaaataag
     8461 gagcatggca agtgaaggaa tgttaacagc agacattgta aaaaactcat tactttcagc
     8521 agcagagcag accaacgcag aatttgagaa gatgccttat acattaggtc aaatctttac
     8581 tagtgtaaaa aataatgcag ttatgatatt tggagctata cagaagaaaa ttgaggatac
     8641 agtttcaagc aagggattta gaactttcat aactgatgtt aaagactcat tatacgtact
     8701 tggagcagtt ggttttaatg tatttagtgg atttattaat ttactgagta gtccagcttt
     8761 tcagaatttt tttaatgtga tgattgttgg aagtagttta gttgtacaag ggctaggttg
     8821 gataataaca caagcactta atgtagctaa tgtatttgca cagaattggt caatcattgc
     8881 tcctgtgatt tggggaatca ttgcagtaat agctatttat aaaataacta ttatttctct
     8941 cttggcaata caaacactgc atacaacatt aaccctagca caaagctttg caacagcttt
     9001 attgaatggt gaattaatgg cagaaactag atttttgtta cttaataaat tagaaacgct
     9061 aggtttaagt caagcaaatg ctttattgtg tgtaagtatt ttaaaagttg ttgctgtaat
     9121 ggctctagta atagcagcaa tatttgtagg agtagcaata ttcaatcatt ttgcaggaac
     9181 aagcatttct gcaactggag tagtagtagg gtgtttttat ttcttgggag cttgtattta
     9241 tgatgtattc gcagcagctt ggaatattat aatggcattt gcagagttct ttgttaattc
     9301 atttaatatc gttatctaca atgtacagtt attattttat aaattccaaa attttgtaat
     9361 agattctatg ggtgacgttg gaggaagttt tgacaattgt gctacagctt tagcaaatgc
     9421 ctttgtaagt gctgctaata tagcaataaa aggtataaat ggagttataa aaacactaaa
     9481 tttgatacca ggcgttaata taaagacatt tggaagcttg gacaaaatag attcttttgt
     9541 aaaacaatat aaagattacc aaaaaacgtt aaaagagcct gtaaagcctg ctgaatggaa
     9601 agctccaact atgaaattga agaatcctgt agattcattt aaaaaagggt atgaagtagg
     9661 tcaaaattta gaaaataaat tgaaagatgc ttttgacatt aacaagatag cagaaaaggc
     9721 aaaaaaagac ttaagattag acgatttatg ggacaaaaaa tatggacttg gtgacggact
     9781 cggttcggca gggcttaatt ctccgctcag cgatgcagca aaaggagcaa aagacacggc
     9841 aggcaatact gcaaagatgg caaagactat ggataaaagt caagaggact taaaatatct
     9901 tagagatatt gcagaacagg aaacaataaa ccgttttaca ggagtcaaca ttaaaattga
     9961 catgaacaat acaaacaaca taagtaaaga tgcagatgtt gatggaatag taaacgtctt
    10021 aacagaaaaa ttaaatgatg ccatggttgt ttcagccgaa ggaatagttt aggaaggaga
    10081 gtgagaaaat ggcttatgat ttttatttag atggagtaca attaccaata cctccaccaa
    10141 agttagagat taaagttaca aataaaaaca agacagttga tttgataaat actggagaag
    10201 taaacatatt aaaaaaagaa ggattatctg aaataagttt tgaagcagaa tttacacata
    10261 ataaattacc tttttatcgt ggaactttta gggatgttca attcttttta agtaaactag
    10321 agttactaaa aactgattgt aagccatttc aatttattgt atcgagggaa ttaggtaata
    10381 aagtactatt taacactaat ataaaagtat ctcttgaaga gtatgctatt tcagaagatg
    10441 cagataatgg ctcagataca aaagttgcaa taaaattaaa gcaatataga gattactcaa
    10501 ctaaaaagtt agttattgcc cctcctaaaa atgagactgg tagacctaat gtaaagatag
    10561 agccaaaacg agttgattca gtcaatgcca caaacactaa aactaaaaca tatacagtaa
    10621 aagcagggga tagcctttgg tcaatttgtc agaaacaact tggtaatggt tcattatata
    10681 agaaagtata cgaactaaat aaatctatga tggataaggc aaataagggc aaaaacttaa
    10741 gtaaatacac tatttataaa gggcaggtgt taaaacttgg ttgatgaatt agtgttagca
    10801 aatgatagag atgtaaggtt agtaatagct cattgggaag atttctacga acctgctgtc
    10861 attgatggta tcacatggga gatagaaaga agaggaacac cttctaagtt agaatttaca
    10921 atagttatgg atgatatatt agagttttgc gaaggaaatt ctgtaaggct gtattataaa
    10981 ggaataggta tcttctatgg atatatattt cagaagaaaa gagataaaga aaatcatatc
    11041 aagatagttg cctatgacca gctaagatat tttaagaata aagatactta tgtatatagt
    11101 aataaaactg caagtgaact tgtaaaaatg ttggctaaag attttaattt aaaatacaat
    11161 gtcatagaag atactaagta taaaatatct aggatagaag aaaataaaac actctttgac
    11221 atgatactaa cagcactaga tgatactcta agagagaaaa aagaaatgta tgttttatat
    11281 gatgattttg gaagaataac attaaagaat gttgcatcaa tgaaattaga tactgttatg
    11341 aacaatgatg taatagaaga ttttgactat aattcatcaa tagatagtga tacttacaca
    11401 aagattaaac ttgtgagaga taatgaagaa acaggaaaaa gagatgtgta tattgctcaa
    11461 gactcaacgc atatgaggag ttggggaata cttcaaatgt ttgatacagt agacaaaaac
    11521 atgagtgaag cagagataaa acaaaagtgt gatatacttc taaaactata taataagaaa
    11581 actaagtcat taagtttaaa aaatgtactt ggtgatatta gagtaagagc aggttgttta
    11641 gtacctgttt ttttagattt aggagatatt aaacttcaaa attatatgtt agttgagaaa
    11701 gtaaaacata catttgagaa taattcgcac tttatggatt tgactcttgt tgatggagat
    11761 gaatttgctt cttattcttc aagctcatat tcaagtggaa atactaataa taaagatgaa
    11821 aagaaaaatg gtcctgcaca aagtattatg aaaaaaaata caggtaaaaa agttcctgct
    11881 atatttactg catattatcc aggaaacaat gcaatggaag gtggaaaaac agattgcaat
    11941 ggaaagccac ttgatgtaaa atcaagaact gttgctggtc caatgaatcg agaaggagtt
    12001 aagaaaactt ggtatactga tgattttcta aagaaacatc cagtttttga atatggagat
    12061 aaagtaaaaa ttatacttcc tggtactgcc tatgacaaca aagtatatac agttaaagat
    12121 aatggaggaa gaatatatgt tgaaacaaac ggaacatatc atatagatat actattagct
    12181 aatgctagtg aatgtaaaaa atttggtaga aagaatggct atataattat aggtggagat
    12241 gaagaacaaa catatcaagt tgaaggtaat aaccaaagta gtacaaataa taactctaaa
    12301 gaagataaat taattagtat agcaaaaagt aaactgggtt gtaattatgt gtatggagca
    12361 gaaggtccta ataattttga ttgcagtggg tttactcaat ggtgttataa acaaataggt
    12421 ataaaaattc ctcgtactgc ttctgcacaa agtaaagcag gaaaagcagt agatttaaaa
    12481 gatagaagca agtggaaagc aggagattta ttgtgcagaa ttggaggagg aagtaacaac
    12541 catgttgtga tgtatattgg aaacaatcaa ataattcatt caccacaaac aggagatgtg
    12601 gtaaaaatag aatctgttaa ttcttataga aaaggaaaag catatacaca tgtgagaaga
    12661 tttatataag tgaggtgata aagtgtcaca agaattattg caaataatta agaagactgc
    12721 aatagatgca gtagaaacaa gcaacccaat gcagattgca tttggaacta tagaaagtct
    12781 taatccatta gtagttaaga tagaacaaaa actatctatt ggtgaaattt ttctaataca
    12841 aacagataca tttaaaagat atacagataa aaagatagga gataaattag tcttaattag
    12901 gatgcaagga gggcaacaat a
//
LOCUS       DAELJO010000110        12843 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELJO010000110 DAELJO010000000
VERSION     DAELJO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.110"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..719)
                     /locus_tag="KPB88_002491"
     CDS             complement(<1..719)
                     /locus_tag="KPB88_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG0054760.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEA"
     gene            934..2757
                     /gene="acd"
                     /locus_tag="KPB88_002492"
     CDS             934..2757
                     /gene="acd"
                     /locus_tag="KPB88_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG0054761.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            2972..7270
                     /locus_tag="KPB88_002493"
     CDS             2972..7270
                     /locus_tag="KPB88_002493"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG0054762.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            7474..7935
                     /locus_tag="KPB88_002494"
     CDS             7474..7935
                     /locus_tag="KPB88_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG0054763.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            7965..9119
                     /gene="nusA"
                     /locus_tag="KPB88_002495"
     CDS             7965..9119
                     /gene="nusA"
                     /locus_tag="KPB88_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG0054764.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9126..9407
                     /locus_tag="KPB88_002496"
     CDS             9126..9407
                     /locus_tag="KPB88_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG0054765.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            9397..9708
                     /locus_tag="KPB88_002497"
     CDS             9397..9708
                     /locus_tag="KPB88_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG0054766.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            9730..11670
                     /gene="infB"
                     /locus_tag="KPB88_002498"
     CDS             9730..11670
                     /gene="infB"
                     /locus_tag="KPB88_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG0054767.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            11713..12093
                     /gene="rbfA"
                     /locus_tag="KPB88_002499"
     CDS             11713..12093
                     /gene="rbfA"
                     /locus_tag="KPB88_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG0054768.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12074..12751
                     /locus_tag="KPB88_002500"
                     /pseudo
     CDS             12074..12751
                     /locus_tag="KPB88_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5128 a         1447 c         2480 g         3788 t
ORIGIN      
        1 gcctcatata aaacttctcc aataggttta tttattacaa gagaggctga cttatcatag
       61 actgtatttg ctttattaat aagagcaata taatcgcctt tgtaataatc tataaaactt
      121 gctgctgggt atacagctaa tgatgtacct cctataatca ataaatcagc attacttata
      181 gcagaaattg ttttagtaat gacatcactg tcaagagctt cctcataaag tactacatct
      241 ggttttacta tagaaccaca gttgtcacag taaggaatat tacctcccag atttaacata
      301 gattctaagt caaaaaattt accacattta gtacaataat ttctatgtac agaaccatga
      361 agttctagca catttttact tccagccatt tgatgaagtc catcaatatt ttgagttatg
      421 acagctttaa gcttacccat ttcttcgagt tttgctaaag ctatatgagc attattgggt
      481 tttgcattag gatatattaa tttatcctta taaaattcaa aaaattcttc tggatatcta
      541 acaaaaaaag tatgtgaaac taattgttca gcagtaaatt gtttattgag tttttggcta
      601 aataatccag tagaacttct aaagtctgga atattagatt cagttgaaac acctgctcca
      661 ccaaaaaata caatattatt gtgattagct attaagtctt ttaagctatt tgcatccata
      721 gttacacctc caaattatta tatataaatt gtactacata ttaggtcgtt tatgttttat
      781 taatttaaat aaatatatag tttaataagt taaataaagg gaatataaat aaaatgtata
      841 cagagtataa atagacaata tttactaaaa taacaagttg tcgtataatt atattaaaat
      901 tgttttagtg tataagaagg gcgggggaag tacgtgaaga aaaaagcagc tttagcaaca
      961 ttggctatgt tacccttagg tgtggtaaat gcacatgctg atggagatat aggtatagtg
     1021 actataaatt atttaaatgt aagaaatgaa ccaactgccg aaagtagcat agcctttgtt
     1081 gctaaaaaag atgataaagt tttgattaaa gactcttcta atggatggta taaaataaaa
     1141 gctgaatctg gacaagaagg ttgggcttca tcaaaatata tagcaaaatc aaatagtgac
     1201 tctttaagaa cgtctacaaa taaagaaaaa caagttattt caaatagttt aaatatgaga
     1261 aatggagcag gtacaagtta cagagtaata actgtactaa aaaaaggtca aaaagtagaa
     1321 gtaatatcag agagtaatgg atggtctaaa atcaaatatg atggaagact aggatatgta
     1381 tctagctctt atttaggaga cgtttcaaac tcaactaata aatcaaaaac taaacaagtg
     1441 aatacaactt cactaaatgt aaggagtgga ccaaatacaa gttatggttt gttaggcaag
     1501 ttgccaaaag gaagtaaagt agaagtaata tcagaaagta atggatggtc caaaataaag
     1561 tataatggaa aagatgccta tgtatctagt atgtacttat cggatgtaag tcaaagtaat
     1621 tcagacaatt ctagtcaaag caatgataaa aagaatactg ataaggtcgt aaatacagct
     1681 tctttaaatg taagaagtgg accaggttct acatatagta agttgggaaa agtttataaa
     1741 ggaagtaaag taactgtact atcagaaagt agtggatggg ctaagattaa ttttaacaat
     1801 aaagaagcat ttgtagtagg caattattta tctacttcag cagatacttc aaataataac
     1861 tcaaatagta actctgataa cagttctaat agtaatggca ataattcttc atcatcaggt
     1921 caagtaaatg gtatgtcagg tataagtggg gctaaaattg attataaatc tttgagttat
     1981 actttagaat ctcatataag taaacaagtt gagaaagcag catcaggagg aaatgtgata
     2041 gctccaagta ataggaaaag cactccaagt cctgaattta gtactttttc agctcaaaga
     2101 acaagctcat ttgtaaatgc aagttcaagt gatatagaat attatttaaa tcctaaaaat
     2161 tttacaaata ctactaaagg tatgatgcag tttttaaaga ttaacagtta tagagatggt
     2221 atttcagaat caagtcttaa ctcgtatctt aatggtttat cttctagtgt atttaaaaac
     2281 cagggagcag cctttataaa tgctgccaag aagtataata tagatgttgt atatctagta
     2341 tctcatgcta tgtgggaaac tgcttatggt aagtctacac ttgcccaagg tcaaacatta
     2401 acatcttata agggacaacc tcttagtaag ccagttaaag tatacaattt ttttggtata
     2461 ggagcaatag ataaaagtgc taatgtttca ggtgcagaag cagcgtattc caatggctgg
     2521 actagtgtag aagctacaat agatggctct gcaaagtgga tatcacaaaa ttacgtaaat
     2581 agttctaagt acaatcagaa tactatctac aagatgaagt ggaattatga ttatacatgg
     2641 catcagtatg caactgatgt gaactgggct aatggaattt ctggaattat ggaaaattta
     2701 attggtcttt atggaggagg aagtagtttg gtttttgaag ttcctcaata caagtaatac
     2761 ataaataaaa aatatagact atgggtagtt ttcattttat ttaaattatc tatagtcttt
     2821 ttgtatataa tttataatct gtaaatttaa tgttaacaaa acttttatgt tataatatta
     2881 attctaagta aatataagtg agtaataggt agaaatagat aaaaagtggt aataatatat
     2941 ataaagataa ttttgagaaa ggaggatata tatggagagt attaaggaat atttggataa
     3001 gttagaaatt aataatagtg gtcttggaaa gcaacttaaa gaagtttata taaatagagt
     3061 agtttatttt aaggaagata agatagttta tttttatcta acttctaaag atattgtttc
     3121 tcatgaactt ttagataaat ttaaagaaga attgatgtat aaactcgatt attttaaaga
     3181 tatgaggatg aagataagat ttactggtct tgaaagaaaa tccaataaag atgttataaa
     3241 aaaatattgg aataatatat tgtatatatt aaagtatctt tgtccatcta ttgcagggtg
     3301 gtataaacaa gtagaatttc tttgtttaga agaagaatta aaaataaaac ttccaaaagg
     3361 tatattttat gagcgattaa tgaaaaaaaa tgtaacttat gtacttaaaa ccgtgcttag
     3421 cgaagagttg ggattagatt taaatataac aatagaaaaa gctgttgatg aaaaggtaaa
     3481 taaagaaaga ctcataagaa tcaatgatag agaaatggaa gaaaaaatta gggctttaga
     3541 aattggaaaa gttaataact gtgaagaaaa tgaagaggaa agttatgtca ttaagtctga
     3601 tgtagatgaa aatttaatct atggagataa tgcaaatgct atgattgaaa atatagttga
     3661 attaaatgca tcttctggaa cagttgcagt tgttggagat atatttgatg ttgacactaa
     3721 agaacttaaa aacggaaaaa tacttatgat agcttcaatt acagattata ctagctcaat
     3781 aagctgtaaa ctttttttaa cagatacaaa taaggatgga gtacttgaaa atgttaaaaa
     3841 aggagcatac ttaaaaatca agggtgacat agtttatgat acatatcaaa gagaaattag
     3901 tatgatgatt agtgggataa gaaaagaaac tagaatagag agagaagata aatcagagga
     3961 aaaaagagta gaactccatg cacatacaca aatgtcttct atggatgcta tttgttctgt
     4021 taaaaagctt gtagagcgtg cagctaaatg gggtcattct gctattgcta taacagacca
     4081 tggtgttgta caaggatttc cagatgctat gaatgcaggt aagtcaaata atataaaaat
     4141 actttatgga gtagaagggt acttggttga agatgattct ctcattatag aggatgcaaa
     4201 tgataaagaa ctttcacaga catttgttgt atttgacata gagacaactg gtttttctaa
     4261 tacaaatgat aaaataactg agattggtgc tgtaaaaata gaaaacttta agattgtaga
     4321 tagatttagt gagttaataa atcctgaaaa agatatttct tataagatac aggaattgac
     4381 aggaataact aatgaattaa ttaaagacaa gccaaccatt gaagaagttc ttcctaagtt
     4441 tatggaattt gttggagata gtgtattagt agcacataat gcagagtttg ataccggatt
     4501 tatatcgcaa aaatgtaggg aacaaggctt gttatataat aacaaaaaag tagatacact
     4561 tatgttagca agagtaatgt tgccaaactt aaaaagacat agattaaatg tcgttgcaaa
     4621 agaacttggt attcctcttt taaatcacca tagagcagtg gatgatgctg aggctacagc
     4681 acttatattt aataagtttt tgcagatgct tacagaaaaa ggtgcaaaaa ctctaagtga
     4741 tgtaaacaat atacttggaa agattgatta cacaaagctt agcacaaatc atataacctt
     4801 gatagcaaaa aattctatag gtataaaaaa tttatataaa ataatttcag atgcacatgt
     4861 aaatcatttt tttagagcac caagaatcct tagaagtgta ttaaatgaat ataaagaagg
     4921 attaataatt ggttcggctt gtgaagcggg ggtggttttt caagctgtaa agaaaaacgt
     4981 gtctgatgag gaaatgaaaa aaataataga tttatacgat tatattgaag taatgcctat
     5041 agataataat agatttatga ttgataaagg agaagtaaag gatgaagaag agttaaggga
     5101 gttgaatagg aaattaattg atacagccaa aaaatttgac aagattccag tagctactgg
     5161 ggatgttcat tttttagaca aacatgaagc tgtcttaaga aaagttctta aatattctca
     5221 aggttttaaa gtagatgaag aagaaactta tttacatttt agaacaacag atgaaatgtt
     5281 ggaagagttt agttatcttg gagaagaatt ggcatatgaa gttgtagttg aaaatagtaa
     5341 tctgattgcc gatatggttg aagatataaa acctatacca gatgatactt atcctccaat
     5401 tatagagggc tcagatgtgg agttaagaga aatgtgttac aaaaaagcca aacgaatcta
     5461 tggagaccca ataccagaaa tagttcaaaa aagactggat agagaattaa attcaataat
     5521 aagcaatggg tatgcagtta tgtatattat agctcaaaaa ctggttgcaa aatctctttc
     5581 agatggatat ttagttggtt caagaggctc agttggctca tcttttgcag ctactatgag
     5641 tgatataaca gaagttaatc ctcttccagc acattatatt tgtgaaaacg aagactgtaa
     5701 atattcattc ttttatgaaa taggtgaatg gggctcaggt gttgatttac cagacaagga
     5761 ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat gatattccat ttgaggtttt
     5821 cttaggtttt gaaggtgata aggaaccaga tatagactta aatttctcag gaaattatca
     5881 gccaacaatt cataaataca cagaagaact atttggagag gggtatgtat atagggctgg
     5941 gactataggt actgtagcag aaaaaacagc ttttggatac gcaagaaaat atgtggaaga
     6001 aaataatata agtgtaccaa atgcagaagt acttaggctt tcaaatggat gtactggagt
     6061 aaaaagaacc tcaggacaac atcctggtgg agttatggtt ataccagact ataaagatgt
     6121 atacgatttt acacctatac agtatccagc aaatgatact tcttgtggtg ttataactac
     6181 tcactttgat tatcattcaa taagtggtag aatacttaaa cttgatatac ttggacacga
     6241 cggtcctacc attataagga tgcttgaaga tatgactgga ataaatataa cagaaattcc
     6301 tttagatgat aaagagacta tgtctctatt tacatctaca gaagcattag gtgttacacc
     6361 agaagaaata aactgtccta taggttgcct tgctgtacca gagtttggaa ctaagtttgt
     6421 acgacaaatg ttacttgaca caaaacctac tacatttgca gaacttgtac gtatatctgg
     6481 tctctctcat gggacagatg tttgggtaaa taatgcacaa gacttagtta gagaagatat
     6541 agttggatta aaagatgtta tatctacacg agatgacata atgaactatt taatatttaa
     6601 aggattacct cccaaaatga gctttacaat aatggagagt gtaagaaaag gtaaaggttt
     6661 aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt ccagaatggt atataggttc
     6721 atgtaaaaag attaagtaca tgttcccaaa agcacatgct gttgcttatg taatgacatc
     6781 atttagaatt gcatattgta aagttcacta tccagaagca ttttatgcga cttattttac
     6841 gacaaaagtt gaggattttg atgcagattt aattgttaaa ggattagatg ctataaaatc
     6901 aaaaataaat gaaattgagt cactgggaaa tgatgcaacc acaaaagaaa aaggtatgtt
     6961 gactgtttta gaagttgctc ttgaaatgta tgctagagga ataaaattat taccagttga
     7021 tatatataaa tcagatgcaa ctgagtttat agttgtagga gaaaaaacat tacttccacc
     7081 aatggcagca atacaaggtc ttggaggaaa tgcggctata aacatccaaa atgaaagaaa
     7141 aaatggtgaa tttatatcaa aagaagattt gagaaagaga acaaaaatat caaaaacggt
     7201 aatagaaact cttactaatc atggttcact tgagaatatg agtgagagaa atcaattgtc
     7261 attgttttaa gttagcccaa aaagttgcaa ataaaagaat tttatgatat catagtatag
     7321 aaaatagtat actttgcaga aactaagagt gagacagctg ttctcactct tttattttaa
     7381 atatattatt aaaaagctgg tttattttta ttaaatatat tttatttaag attatataaa
     7441 ctggtcattt acaattgaat aggaggaact ttaatgaaaa aaaatttaga agctacaatt
     7501 gaggagattg taactaaaat aacggatgaa catggatttg aaatggttga tgttgaatat
     7561 gtaaaagaag caggagaata ctacttaaga gtatacatag ataaagaaga aggaatatct
     7621 ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa tactagatga aaaagaccct
     7681 ataaaagaaa attacttttt agaggtatct tctccaggac ttgatagagc attaaaaaaa
     7741 gacagagatt ttgttagata tcaaggtaga gatgtagatt taaaattata taaaccatta
     7801 aatggatgta agcaatttga aggtgaactt gtaggattaa cagaagataa taatataaaa
     7861 atcattgcaa atggtaagga aatggaattt aatagaaaag atgttgctat tgtcagatta
     7921 gcaataaagt tttagtggta tattaaatag ggaggatata aaaaatgaat catgaattta
     7981 tggaagcatt agatgaatta gtagttgaca gaggtataga taaagaaata cttatagata
     8041 ctatagaaca agctctttta actgcataca agaaaaactt tggttcagca caaaatgtta
     8101 gagttgaatt tgatagagac aagggagata taaaagtatt ttctcaaaga gttgttgttg
     8161 atgaatcaga tttatatgat actttcttag aaatagagtt agcagatgct agagagataa
     8221 accctaatta tgaattaggt gatattatag aacatgaagt tacacctaaa aactttggaa
     8281 gaattgcagc tcaaacagca aagcaagtag ttgttcaaag aataagggaa gctgagagag
     8341 aaatagtgta taatgaattt atagaaaaag aaaatgaaat agttacagga gaggttgcta
     8401 gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat agaaggtata atgactcaaa
     8461 gtgaacaaat accatttgag aattataaag caggtcaaaa aataaaattc tatatacttg
     8521 aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc aagaagtcat ccgggtctag
     8581 taaagagatt gtttgaagaa gaagttcctg aaatatatga aggaatagtt caaataaaat
     8641 ctgtagcaag agaagcaggg tcaagaacta aaatggcagt aagatctata gatgaaaaaa
     8701 tagacccaat tggagcttgt gttggaccaa aaggacttag agtaaaaaat atagtggatg
     8761 aacttggcga tgagaaaata gatataataa aatatagtga agacccagca gagtttatat
     8821 cagcagcact tagcccatca aaggttgtta gagtagatat agatgaagat gaaaagtctg
     8881 ccttagtaat cgttccagat tatcagttat cgcttgctat aggtaaagaa ggtcaaaatg
     8941 caagactagc agcaaaattg actaactgga aaatagatat taagagcgaa tctcaagcag
     9001 aacaagaaat gttaaatctt gaaaattcca aagaaataga agctgaggat gttgaagctg
     9061 aatttattga ccaagaagag ttagatactt cagaggataa ttttagtgaa gaagagtaag
     9121 gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag aaaatgcata gcatgtcaag
     9181 atagagactc taaaaaagag cttataagaa tagttaaaaa taaagaagga aaaatatttt
     9241 tagacccaac aggaagagcc aatggaagag gagcatatgt atgtaaaagc tctgaatgtc
     9301 taaaaaaagc tataaagagt aaagcattaa ataaagcttt taaaatagat gttcctgatg
     9361 aagtctatga taatctactt atggagttag aagaatatga aaaataataa agaaaaagta
     9421 ctttcgttct tagggctcgc aactcgtgca gggaaaatag tgtctggtga tgattcaact
     9481 ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa tcgcagaaga tgcatcaaat
     9541 aatactaaaa aactttttaa agataaatca acttttagaa atattccata tctatttttt
     9601 tcaacaaaag aggaaatagg atttgcaata ggaaaatctc caagagcagt tgtaggaata
     9661 aaagatgaaa atttttctaa aaagataatt gaactaatag aaatttaaaa taaaataggg
     9721 ggtgatcttg tgtcaaaaac aagagtatac caaatagcag aagaattaaa tatatcaaat
     9781 gaagagctta taaataagtt agctgaatta gatataaatg taactgacaa agattcagta
     9841 ttagaaggtg aagagttaga attagcactt gaaatgttag gtgaagattt atctcaagaa
     9901 aatggaaatg ttatagaaat agatggaaag ttgacagtac aagtattagc aacgaaatta
     9961 gataaatctc catcagaaat aattatgaag cttatgaaaa tgggaactat ggcaacaata
    10021 aatcaagaga taagttttga gattgctgct ttggctgcca aagactatgg atttgaatta
    10081 acagtagctg aaagtgatga tacagaagct ttagaaatag aagctttaat ggaaattgaa
    10141 gaagataaag aagaggattt aaaacctaga ccaccagttg ttacagttat gggacatgtt
    10201 gaccatggta agacatcttt actagatgca ataagaaaaa ctgatgtgat ttcaggtgaa
    10261 gctggaggaa taactcagca tataggtgct tcagaagtta aaatcaatgg acacaaaata
    10321 gtatttttag atactccagg acatgaggct ttcacatcaa tgagagcaag aggagcacaa
    10381 gttacagata tagcaatatt agtagttgca gcagatgatg gtataatgcc tcaaacagtt
    10441 gaagctataa accatgctaa agcagcaggt gtacctttaa tagttgctat taataaaata
    10501 gataaaccag gtgcaaaccc agataaggtt aagcaggaat tagcagatca aggattatta
    10561 gttgaagact ggggtggaga agtaatagca gttccagtat cagctaagaa aaaagaagga
    10621 atagatacac ttttagaaat ggtattattg gttgctgaaa tggaagagtt aagagctaat
    10681 ccaaataaaa gagcagtagg aactgttata gaagctgagc ttgataaagg aagagggcca
    10741 gttgcaacag tacttgttca aggtggtact ttaacagtag gtgacccaat cgttgcaggt
    10801 gttgcatgtg gtaaagttag agcaatgata aatgcaaaag gtaagagagt aaaaacagca
    10861 ggaccatcta cagcagtcga gatattgggt ctatcagaag ttccacaagg aggagaccaa
    10921 tttgtcgaag ttccaactga taagattgct agaagtgtag cagcaagaag acaacaaata
    10981 gttagagatg aaatgttaaa atcaactcaa agattatcac tagatgctct atttagtcaa
    11041 atgagtgaag gttctataaa agaccttaat atagttataa aagcagatgt acaaggttca
    11101 gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg aagaagttca agttaaagta
    11161 atacatggtg gtgttggagc tgtaacagaa tcagacatat tacttgcagc agcttctaac
    11221 gcaataataa taggattcaa tgtaagacca gttccaggtg cagaatctct aggagaaaaa
    11281 gaaaatgttg atataagaac atatactata atatacaagg caatagaaga tatacaagcg
    11341 gctatgactg gtatgttaga ccctgaatat gttgatgaag aaacaggaaa agctgaaata
    11401 agagaaatat ataaaatatc tggtgttgga acagtagctg gatgttatgt gactaatggt
    11461 aagatattta gaaattgtaa agtaagatta gttagagata gcataataat acatgaaggt
    11521 gagcttgcag cacttaagag atttaaagat gatgtaaaag aagttaattc tggatacgaa
    11581 tgtggtatga gttttgtaaa ttataacgac ataaaagaag gcgatatagt agaagcttat
    11641 ataactaaag aagtagaaag aaaattataa attcgtatca aagaaaatca gttagtaaag
    11701 aaggtcgatg gtatggcatc atataataga acaagaagga tagcagaaga aataagaaaa
    11761 gtcgtaagta ctatgcttat aaatggagta aaagacccta gaataacatc tatggtgagt
    11821 gtaactgatg tagaagttac aaatgaccta agatatgctt atgtatatgt aagtattcta
    11881 ggcggagatg aagaatccac actaacaggt cttaaaagtg ctggaggata tataagaaga
    11941 gaagtaggaa aaaatataaa acttagatat attcctgaga tagtatttaa acttgatgat
    12001 tcaatagaaa aaggaatgta tatggatagc cttataaaaa gagttaatga aaaaaatgct
    12061 caacaaaatg aggatgaaaa ttatgatgaa taatatggat agtattataa actatattta
    12121 tgagggtgac tattttatag ttacctctca tatcagtcca gatggagaca atattggttc
    12181 tacactttca atgtattata cattgaagaa tttaggaaaa aatgtatatt atgtacttga
    12241 tgatgaagct ccattaaatt taaggttttt ggttgaaggt gtaaaaatct ataagtctaa
    12301 tgaatttaat atgaagaact attctctaat tgcacttgat tgtggggata agatgagaat
    12361 atgtgtgagt gatgaaatca aaaataatgc atcaaaatta atctgtattg accatcatgc
    12421 tagtaatgat tcatatggag atttaaatta tgtagatatt gatacatctt ctacatgtga
    12481 actagtttat aatttattag ttagatttca gcaaataaaa gatattaata ttataaatga
    12541 agatatagca acatgtttat atacaggtct tgtaactgat actggaaact tttcgtattc
    12601 aaatgtacat gcaagtagct ttgaaatggc aaaaaattta cttgttttag gtgcacaaaa
    12661 aaatactata atacaaaata tatatcaaag caattcttct ggttattaca aattattagg
    12721 agaagctcta aagggtttgg agatatttga ttaaaaaata gttacgcaca ctcgtgactt
    12781 tagtcatgag ttagtagcta aaatagtcag catataggga aacttatatg tagagatagg
    12841 ata
//
LOCUS       DAELJO010000111        12799 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAELJO010000111 DAELJO010000000
VERSION     DAELJO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.111"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            58..1131
                     /gene="nth"
                     /locus_tag="KPB88_002501"
     CDS             58..1131
                     /gene="nth"
                     /locus_tag="KPB88_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG0054769.1"
                     /translation="MKNVNLKEINEKDVIVEEEINLKGMDKKDIIEKKEINKKEMDKK
                     DIIEKEEINKKGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSI
                     KVSKKSTKKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKL
                     YPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISK
                     EIKSCGLYKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNH
                     PAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKAR
                     NPECASCPIKEDCNYYKELNETK"
     gene            1325..1795
                     /gene="trmL"
                     /locus_tag="KPB88_002502"
     CDS             1325..1795
                     /gene="trmL"
                     /locus_tag="KPB88_002502"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG0054770.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1831..2679
                     /locus_tag="KPB88_002503"
     CDS             1831..2679
                     /locus_tag="KPB88_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0054771.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            3035..4039
                     /locus_tag="KPB88_002504"
     CDS             3035..4039
                     /locus_tag="KPB88_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0054772.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGVKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRTKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKIIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            4165..5277
                     /locus_tag="KPB88_002505"
     CDS             4165..5277
                     /locus_tag="KPB88_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0054773.1"
                     /translation="MKIKRILMVCFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDIEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            5766..6527
                     /locus_tag="KPB88_002506"
     CDS             5766..6527
                     /locus_tag="KPB88_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054774.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            6530..7348
                     /locus_tag="KPB88_002507"
     CDS             6530..7348
                     /locus_tag="KPB88_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG0054775.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            7528..8130
                     /locus_tag="KPB88_002508"
     CDS             7528..8130
                     /locus_tag="KPB88_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG0054776.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    8691..8929
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8993..10912
                     /gene="thrS"
                     /locus_tag="KPB88_002509"
     CDS             8993..10912
                     /gene="thrS"
                     /locus_tag="KPB88_002509"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG0054777.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            11680..12771
                     /locus_tag="KPB88_002510"
     CDS             11680..12771
                     /locus_tag="KPB88_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0054778.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         5266 a         1208 c         2332 g         3993 t
ORIGIN      
        1 tgattaaact atttataaat agcgttttat aaattagtat tttaaagggg gataattatg
       61 aaaaatgtaa atctaaaaga gataaatgaa aaagatgtaa ttgtggaaga agagataaat
      121 ctaaaaggaa tggataagaa agatataatt gagaagaaag agataaataa aaaagaaatg
      181 gataagaaag atataattga gaaggaagag ataaataaaa aaggaatgaa taagaaagat
      241 ataattgaga aggaagagat aaatgaaaaa gaaatagata aaatgattgg taaaaaagat
      301 gagataattg atgagaaaga tatagagaat tttatggata ttgaagaaga taaaaaaagt
      361 atcaaagtaa gtaaaaaaag tactaaaaaa ggtgaaacgg agaaaaaaac aaagaagagt
      421 agttctaata aggctacaca aaaaacaagt aaagaaacta aaagtaagtc taaaattaaa
      481 aatgagtcag caaatgaaaa agacgtaaat aagattttag acgagttaga aaaactgtac
      541 ccagatgcta aatgtgagtt aaattatggg acagcctttg aacttttaat agcaactatt
      601 ttatcagcac aatgtactga tgtaagagtg aataaagtta cttcagagtt gtttaaaaag
      661 tacaacacag ctagagactt tgctaatttg agcattgaag aaattagtaa agagataaag
      721 agttgtggtc tttacaaaag caagtctcaa aaaataaagg atacatctga acagctatgt
      781 gaattatatg atggtgaagt acctgatagc ttagaaaagc ttataaaatt acctggtgtt
      841 ggaagaaaaa cggcaggggt tgtacttagt aacgctttta atcatccagc aatagctgta
      901 gatactcatg tatttagagt tagcaataga ataggaatag tggacgagcc aaatcctcaa
      961 aaaacagaat ttgcacttat ggaagctata ccaaaagaaa gatggtcaca ctcacatcat
     1021 gtattaattt ttcatggaag aagaatgtgt aaagctagaa atccagaatg tgcaagttgc
     1081 ccaataaaag aagattgtaa ttattataaa gaacttaatg aaacgaaata acttttagaa
     1141 agtaagttgt acaagaggga ttttctgata taataatact tgtgaaaatg ttagataaaa
     1201 aactattata tacatgaaat aaaaattatt attaacatta ttttatataa tagtataata
     1261 ttattgttat tctgtttata aaataaaatt tgataaataa aagcaatact tagggaggat
     1321 taaaatgtca ttaaatatag tattagtaga accagaaata cctcaaaata caggaaatat
     1381 aataagaacc tgtgcagcaa caggttcaac tttacattta gtaaaaccac tgggtttttc
     1441 attggatgat aaacacctaa aaagatgtgg gcttgactat tgggatatag ctgatataca
     1501 atattacgat agctttgaag aattacaaga aaagtatcca aatagtaaat actttttttc
     1561 tacaacaaag gctaaacagt cacattcaga tgtgaaatat gaagaaaatt gttttatagt
     1621 atttgggaaa gaaacaaagg gattaccaga acctttgttg aaagcaaata tggatacttg
     1681 tattagagtg cctatgttgg atgttgaaaa agctaggtca ttaaatttat caaattcagt
     1741 tgctattgta gtgtacgagg ctttaagaca aataggatac ccaaatatga ggtagtttta
     1801 acaagcctaa atgagaaagg agcatactta atgaaaaatc taaagataat ctgtgacagt
     1861 ttatcggacg tgccaaaaga tttgttagat aaatatgata tagatatagt ttcattaaca
     1921 gttatactgg aggaaagaga atacaaggat agaattgata tatctggaga tgaattttat
     1981 aaaaggctta gagaagaaaa tgtatatcca aaaacttctc aagctactta tgcccaattt
     2041 aaggaaattt ttgagaaata tacaaatgag gatagagaga tactttatat agcagggtct
     2101 gccacagcta caggtacgta tcaaagtgct gtaatggcaa agaatgatgt agatggtaaa
     2161 atatatacat atgactctaa cttgctttgt atgggaactg gtttacttgt aataaaagca
     2221 ggagaacttg caagtgaagg aaagtctgtt gctgaaatat tgcctgtttt ggatgaatta
     2281 aaagagaagg cttttgttat attttcagta gatacattgg aatatttaca aaagggaggt
     2341 agaatatcat ctactaaggc agcaatagga agtatattaa atataaaacc gattcttgaa
     2401 ataagagatg gattagtttc gcaaatatct caagtaagag gaaagaagaa tgtaatttct
     2461 aagatgatgg aattgataaa atcaaattgt ggtgatgatt tatcagacca aattgtgtat
     2521 ataggatata gtgatgattt taaggaaaaa gaacaattat ctaaggttat gcaagatgaa
     2581 ttaagaccta aagaaattag atttttccaa attggtgtgt gtataggtgc acatgctggt
     2641 cctggtgtta ctggtatcat atgcttaaaa aataaataat atatttttgg atgacattat
     2701 ttattttttt attaaattag gatagaatat taattattga gatgttaaat tggatttatt
     2761 ttggatgaaa ttaagtgttg atatattaga tttagtttgt agggaagtgt gtaaatagat
     2821 aagaatctat tgcacacttt tttatagtaa gatttattaa aaaaatttca aaatgtactt
     2881 gtaaattgta tataaaaaaa ttataaatag gatgtaaaag taaataggat gtaaaagtaa
     2941 ataggatgta aaagtaaata ggatgtaaaa gtaaatagga tgtaaaaata aataggatat
     3001 aaaaatatcg taaatgtatg aggagtgaaa tttaatgcgt ttcaaaagaa tattgatatt
     3061 gatgttaact gtagcaatga tagctggaat gttggttggg tgtgctaata acccaagtaa
     3121 taatcctagc tctagcaaag ataataaaag tgatataagc gataaaagta attcaaaaaa
     3181 atctgtatca atgatactag atattgaagg aacaaataat gaagctatga ataattcagc
     3241 tttattagca ttaaacaatg ctcaaaagaa acttaatata gatacaaaca aagtggaatc
     3301 agatgattcc tcaacttttt ctaatagtat agatatttta tgtaatgaca attacgattt
     3361 aattatagct gtaggagcta gatttgcaaa accattggag atggtagcca agaaatatcc
     3421 aaagcaacaa tttgcaatta tagactatga gtatgacaag caaccttcta atataacttc
     3481 aatttcttat gaagataata aatctggata tctagctgga ttaattgctg gaaaaatgac
     3541 tgaaagcgat aaggttggtt ttataggagg agtaaagagc tctagtaggg ataaatttga
     3601 atcaggtttt agagagggtg ttaaattttc taatagtagt ataaaggata tttcagttga
     3661 gtatgcagat gtttttaaag atagcaaatc agtagaatct atagctaaaa aaatgatgga
     3721 taatggagtg gatattattt tttctactac tgaagatgat tcaaaagctg taatagatgc
     3781 agtaaggaca aaaaataaaa aagttatagc aacgaataaa gaccaacatg agttagctcc
     3841 agaaaatgtt ataagttcta ttgtaaaaga ttttgagaat cctacttata atctgataca
     3901 aagctttgta aaaggaaatt ataaaggtgg aaaaattata gaaaacacag ttaagtctgg
     3961 aagtactggg cttgcccaaa atagttctca aaatattcct ccagatgtgt tagaatatgt
     4021 taataagaac aataaataat ctactataat atccaagaat acttatgact ttagttaaga
     4081 ggtgaattgg atgttataat agacatatat agaaagacat atgcagaggt aggttagacc
     4141 tatctaaatc agggagtgaa ttttgtgaaa ataaaaagga tattgatggt ttgctttgta
     4201 gtagttttta caatttttat attagctata tgtaattttg attttgggtt tgataaagta
     4261 aataagaata attctaatat agatagtgct aaatcgaaac agagtgagtc taaaagtttg
     4321 ccaacttcag cagaaaagga taaatcaaat agtaaaagta agaataaaga agatgacaca
     4381 gaagatgatt catatgtaag aaaaactatt ggagtattgt tagataccga aaacttaaat
     4441 aacgaatcat ataataacaa tgtactatca gccttaaaaa gggctgacga agattttgat
     4501 gttaggataa aacttgaaaa accaaagact tctaaaagtt ttaaagagag tatagaaaaa
     4561 ctgcataagt atgaaccaga tttgataata gcagttggag ccagatttgc agagccaatt
     4621 aaagaagctg ctactaaata tcccaaacag caatttgcaa tagttgacta tacttataaa
     4681 gttcagccag ctaatgtaat ttctgtatca tttcaagata atgttactgg atatttagca
     4741 ggacttattg caggtaaaat gactgatact gggaaagtcg gttttatagg aggagttgag
     4801 ggttctagta gagataagta tgaatctgga tttagaaagg ggcttaaaac ttcaaataaa
     4861 gatgctgagt tactagttaa gtattcaaat atatttgaaa accctgaatc agctagaact
     4921 attgcaaagg aaatgagaga ttctgataga gatgttgtat tttcagctac agaggatgat
     4981 agcaaaagag ctattcaggt agcaagagaa aatggtggta aagttatagc tattaataaa
     5041 gaccaatacg atatggctcc agataatgta ataagttcaa ttataaaaga tatagaacaa
     5101 cctgtatatg aactgataaa gaacgttgca aaagatggtt ttaagggaag taaagtaatg
     5161 gaatttaaaa taaaagatgg ggaacttggc ttgacttcaa agagcagtag aaatatacca
     5221 ccagatgttt tggaatacat aaaaaaagag tataacaaag tgaaagatac atattaatta
     5281 ttgagtagaa gattataaaa atatactgct taagtttaga ttggtgataa aaggatatat
     5341 ttattaaatt tgtgctaact gtacaaaata tattgattaa gtttagatta gtgataaaaa
     5401 gatatattta ttaaatttgt gttaacagta tggaatatac tgcttaagtt tagattggtg
     5461 ataaaaggat atatttatta aatttagtct aactgtacaa aatatattga ttgagtttag
     5521 gttagcaata aaaagatata tttattaaat ttgtgctaac tgtacggaat atactgctta
     5581 agtttagatt agtgataaaa agatatattt attaaatttg aaccaactgt acaaagctat
     5641 actaattaag cttagattaa ctatataaaa ttataacaat aagtgtgtgg gcagaaaaat
     5701 tctgctacac actttattta tattcacata aaaaatttat caaaaaaaag aaaaaggagt
     5761 aaaaaatgga atttacaaga gaaccattta tactagatga cgatttgtgt ctagctttgg
     5821 tggacaaaag aattagttat ctaatggaaa atgaattaaa attaagaaat atagagataa
     5881 taaaaacaat agaatgtaaa gaattatatg aaagcataaa atatcacccg gatatatgta
     5941 tttgtaattt aggaaaagga gatataattg ttgcgccgaa tgtatataat caatacaaag
     6001 agttattaag tagatataaa tttaatataa ttaagggaga agctaattta ttaaatagat
     6061 acccttatga tataccttac aatattgcga tagttggtga ctatgctata cacaatttta
     6121 agtatactga caaagcaata ttagattata ttgaaaataa taaacttaaa aagataaata
     6181 tagagcaggg ctattctaaa tgctcaatct gtatagtaga tagaacgtct ataataacat
     6241 cagataaagg tatttggaaa agtatgaaga atacagaaat agagtgttta ttgatagaaa
     6301 aaggtcacat aaatttattt gagatgaatt atggatttat aggtggatgt acagggttga
     6361 tttctaagga taaactagct ttttgtggag atgttaaaaa acatcctgac tatgaacgta
     6421 taaaaagttt tgtagaaagt aaaaacaagg agatagttac acttagctgt gaaaatttac
     6481 tagatatagg ttctatagtt cctctcatga caagaaagga gcgataactt tggttgagaa
     6541 ggataatatt tcttccatga aatttttgag gaataataat gtaaacgata gattaatata
     6601 tacgaatgac attgtagaag tagtgataga ttctgcaaag caagaagtgt taaataaaaa
     6661 aatacttaat ggtactaaaa ctgaacaaag agctttaaat attgatgaga tatcaacgga
     6721 aataactagt atgataataa gtataatgaa agagaaactt ttgaaagaat acattctaaa
     6781 atgctatggg cgtacatatc ctgaagataa aaataatata tacatatatt cactgaacat
     6841 atttgctaaa aaagagattt ttatgagaga aacagtattt actagggtga gaaattatat
     6901 agaagaaaat gattttataa atgtagaagg ttttattaga tttagaatga gagaatttat
     6961 gaaatatatt tctgctatag gtgatattgc agttgaggag tatttaataa agaaagacca
     7021 agatgaattt ataagagttt taaaatactt tatagatact caagaagaaa agattgattt
     7081 gcttaaagtt catataatgg aagataatac atttgtttta tacgataaaa atggaaataa
     7141 gatagacagt atagatgatg aagaaattat aaatatggta ataagagaaa atttaaatta
     7201 tgaggacttt ttaataagta ccttattaac tctatgtccc aaaaaaatag aaatattgga
     7261 tttgttaaat aataattgct caaaggaaat tatagatact gtagaagcaa tttttgaaaa
     7321 taaagttagt ataattatgg aaaactagaa tctaagcata taaaagctat ccatataaaa
     7381 ttaaaaaaat tctttttaaa tcgacacaaa agacttataa ttgtatataa agctatagta
     7441 taactagagt tattaagtat aaagaatatc tacttgatta atgcaatata gctggcaaat
     7501 atacaattga taaggagtaa ttttataatg gataatttaa ttaggatact gatattagct
     7561 gttattggtg gatttatagg ttatgttacg aatgtagttg ctataagact gatatttaga
     7621 ccaatagagc ctattaaaat acctatttta aatatagaaa tagtaggtct tataccaaag
     7681 agaagagcag aaatagcagc aaatgtagga gaaataattc aagaagaatt tctttctatg
     7741 gacgaaatac ttgcaaatat cattactgac gaagataagg aagaagttgt aagatacata
     7801 aaagcaaggg taaaaattat aatacatgag aaagtttcat ttatcccgag tggtataaaa
     7861 aacatgatac aagattatct aggagagata attgagtctg aagttaagca aagtatagat
     7921 gaattgagta aaaatataat aaataaagca aatgaacgta tagatataca aaaaatggtc
     7981 gaagataaga ttaatgaact agatttatat gaattggaag agataataat aagaatagct
     8041 aagaaagaat taaaacatat agagtttttg gggcttgtct tagggttttt aataggtata
     8101 gctcaagggt taattactat gtttatttaa atatatgaga agtagcattt atttaagtac
     8161 taattatatt tacctatttg agtgaattac ttattaataa catttagata ttaattgtat
     8221 ttacctattt gagtgaatta cttattaata gcatttagat attaattgta tttacctatt
     8281 tgagtgaatt acttattaat agcatttaga tattaattgt atttatttat ttgattgtgt
     8341 tacttattaa tagtatttaa gtatttactg tactacttat taaccatatt aagtattgat
     8401 tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt agaagttagt tttaataaaa
     8461 attagtttta gtatttatct agtcatatct atcatgcatg tggatacgtt ttaaaaattt
     8521 taaatttatg aaaatattat aatatcacta taaatcaaga gattaattta atataaaaat
     8581 ataagaatat tggtttacag ctgattgaga tgaaaaataa tgggtaatta atatattaca
     8641 ttaatgttga ttgatgtata actatctgtt ataatgaaaa tatattaaaa atcatagtgt
     8701 tgaagaggaa gagtagagta gaaaccattc tcagagaatt tgggtctatg gctgagagcc
     8761 catgtaatag gtctatttga aaactacctt ggagctgagc ttattagcag tatttgtatt
     8821 tgtactatac gtgaaagtgc tcgggtgacg accttatagt catcaagagg gttgtatata
     8881 cttgtatacg ccaacttggg tggaaccgcg aattaatatc gtcccaaaca tatttggggc
     8941 gattttttat tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa caatgattaa
     9001 agttgcttta aaagatggct caataaaaga atttgaaaat gctatttctg ttatggatgt
     9061 agctaaatcc attagtgaag gattagctag aaatgtggta gcagcgtctg tgaacggaga
     9121 ggttgtaggt ctggaccaca ttatagatac tgattgtgac ttgaatttat ttaagtttga
     9181 agataaagaa ggtaaggaag tttttagaca tacatcagca catatattag ctcaagctat
     9241 aaaaagactt tacccagaag caaagcttgc tataggacca agtatagaaa atggattcta
     9301 ctatgatata gatttagacc atagattagt tccagaggac ctagagaaaa tagaagctga
     9361 aatgaaaaaa atagctaaag aagatttaaa aatagaaaga tttgaacttc ctagaaatga
     9421 agctttagag cttatgaaag aacaaggaga agactataag gtagaactta tatctgattt
     9481 acctgaaagt gaaataatat ctttctacaa gcaaggtgat tttactgatt tatgtagagg
     9541 accacattta ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg tagcaggtgc
     9601 atattggcgt ggtgacgaaa ataataagat gcttcaaaga atatatggaa cttcttttga
     9661 gaaaaacaaa gacttagaag agtacttaca tttactagaa gaagctaaaa agagagacca
     9721 cagaaaatta ggtaaagaat taggattatt tatgatacct gaagaaggtc caggattccc
     9781 tatgttttta ccaaaaggta tggaacttaa aaatgaatta ttaaaattct ggagagaaat
     9841 acacagaaaa gctggatata ttgagataga atctccaatc atattaaata gaaaattatg
     9901 ggagacttca ggacactggt atcattacaa agaaaatatg tatacagtta aaatagatga
     9961 tgaggattat gctataaaac ctatgaactg tcctggtgga ttaatttact ataactctca
    10021 actacattca tatagagatt tcccaatgag agttgcagag cttggtagag ttcatagaca
    10081 tgagttatct ggtgcattac aaggattaat gagagttaga gcatttactc aagacgattc
    10141 acatatattc atgttaccag agcaaattaa ggacgaaata aaaggagtag ctaacttaat
    10201 agatggtatc tacaaaactt ttggatttga atataattta gaattatcta caagaccaga
    10261 gaactctatg ggaagcgacg aagagtggga agcagcagaa aatggattaa gagaagcttt
    10321 agaagagtta ggtctaccat atactattaa tgagggtgat ggagcattct atggtcctaa
    10381 gatagacttc catctaaaag attgtttagg aagaacttgg caatgtggta ctatccaatt
    10441 agatatgcaa cttccaagac aatttgataa tacttatatt ggtcaagatg gtgaaaagca
    10501 tagaccagtt atgattcata gggttgcttt tggtagtata gaaagattta taggtatatt
    10561 aatagagcat tatgctggta aattcccagt ttggttatct ccaactcaag ttaagatact
    10621 tcctatatct gataaattta tggattatgc aaatgaagtt aagaaagagt tatttgataa
    10681 gggtataaga gttgaattag atgatagagc tgaaaaaatt ggatttaaga taagagaagc
    10741 tcagcttgaa aaagtgccat acatgctaat agtaggtgaa aaagaagtag ctgataataa
    10801 tgtttctgtt agatcaagag ataaaggtga aataggtagt attaaattag atgaatttat
    10861 agctagtatt tcaaaagaaa ttgaatcaag agaaagtatt attcaagact aatatatatt
    10921 atattagtag ctaagatatt gttatataag ataagtttaa tcaatatgtt gatatgttga
    10981 tgcatagtga ttaaatttat gaaaagatga gtataattag aggtacagtt aatcatattt
    11041 ttatgattaa ctgtacctct agttttttgt atggtagatt tattaataaa tatatcatag
    11101 aagtgttttg aacatggaaa tatttatgtg cattctttaa ataaaagttt atttttgagt
    11161 gataattaag tgcttgttgg taaggttatg gctgtaaaaa tcataacttt attttcatat
    11221 ttgttccatt ttcataaaaa ttattatccc tttaataaaa tttcttaatc ctcttgaaat
    11281 atttcctttt tagtttaaag ttaaatttct ttcttcaaaa tgaatattta aatgaactaa
    11341 aatttaagta cattttctaa tattatgagt ctatttttca tagataattg ccctataata
    11401 gaagaatcta tgttggtgct atgtggtgac aataacagca ttgtcagtga ttaattaatg
    11461 ctttataaat gattaattag tgttttatgt tatttttgga cttaccctct atacatagat
    11521 taatttattt aactttagat atagagagaa tgtcaagtac atacacagac attagagttt
    11581 aagaaagtgt ttatggagca agaactttta aaagaggtag tgacttagtt taatttcaag
    11641 aaatgataaa tacaaaagag gaaaaagaga ggtgttgaaa tgatagaaaa agtagaagaa
    11701 aaatctaaga ttattaaaac atcaaaagaa tataagcgaa ctataattga agaaaaagat
    11761 aaagaatgga ccaaaaaatt aaatgatata cttagtagaa gtagtttttc taatgatgaa
    11821 ttagataaaa tcaaggcgtt aattccaaaa gagattttga ctaatgaaaa tgttggagaa
    11881 gttgtgactg aagatggata tgctaagcct gagtatgatg aggtatttaa atcattgttc
    11941 actttaaata atgaatatga tttacttgta aattttataa atgatatcct aaaagatgct
    12001 ccatatgcta acaggaatat caaaccgttc actcaaataa agagaataat tagagttgaa
    12061 actgatccaa caattaatta cattggagag aaaagaccta gacttgatat tttggctgaa
    12121 gatgaagaaa ataatcatat aaatattgag atgcaaagag cttttgagaa cgattatttg
    12181 gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga aggtaaagag
    12241 tataaagaaa ttggaaaaac tattggaata catatactaa atcatgtaaa atataatcac
    12301 atagaagatt atgtaaattg cctgagatta acaatggacg ggcatccaga tatttattct
    12361 agtaaaactg cattatattt tatagagctt ccaaaaattc ataaatctga ctatatagta
    12421 aatagaatta tgctatgggg aaaacttatt agtaatcctt ctaacttaga tgtaagagtt
    12481 atagctaaga atgattctat tattaaaaaa gcacttgata gattaaaaga attaggtagc
    12541 aatgaagaat atttattaaa tctgaaaaga ggtgcttata tcatgaataa aagtagaaat
    12601 tttaaagaag agattgaaag agagacagca attagagttg caaaagaaaa agattataaa
    12661 ataattataa tgttgaaaaa aaatggtttt aaattagatg acatattaaa taaatttaat
    12721 gattttgact taagtgaaga tgagataaaa gaagtatatg aaaataattg atgataaata
    12781 aaaagtgaac ttatataag
//
LOCUS       DAELJO010000112        12798 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELJO010000112 DAELJO010000000
VERSION     DAELJO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.112"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(46..1044)
                     /locus_tag="KPB88_002511"
     CDS             complement(46..1044)
                     /locus_tag="KPB88_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG0054779.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            1148..1585
                     /locus_tag="KPB88_002512"
     CDS             1148..1585
                     /locus_tag="KPB88_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0054780.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRLLKKINDNFKNAD"
     gene            1671..2258
                     /locus_tag="KPB88_002513"
     CDS             1671..2258
                     /locus_tag="KPB88_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0054781.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(2558..3178)
                     /locus_tag="KPB88_002514"
     CDS             complement(2558..3178)
                     /locus_tag="KPB88_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG0054782.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(3186..3953)
                     /locus_tag="KPB88_002515"
     CDS             complement(3186..3953)
                     /locus_tag="KPB88_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG0054783.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(3994..4815)
                     /locus_tag="KPB88_002516"
     CDS             complement(3994..4815)
                     /locus_tag="KPB88_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054784.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     VNIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(5174..7084)
                     /gene="phnW"
                     /locus_tag="KPB88_002517"
     CDS             complement(5174..7084)
                     /gene="phnW"
                     /locus_tag="KPB88_002517"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG0054785.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSSISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(7363..8148)
                     /locus_tag="KPB88_002518"
     CDS             complement(7363..8148)
                     /locus_tag="KPB88_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0054786.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(8365..8595)
                     /gene="dltC"
                     /locus_tag="KPB88_002519"
     CDS             complement(8365..8595)
                     /gene="dltC"
                     /locus_tag="KPB88_002519"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG0054787.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(8614..9762)
                     /gene="dltB"
                     /locus_tag="KPB88_002520"
     CDS             complement(8614..9762)
                     /gene="dltB"
                     /locus_tag="KPB88_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG0054788.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(9762..11276)
                     /locus_tag="KPB88_002521"
     CDS             complement(9762..11276)
                     /locus_tag="KPB88_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG0054789.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(11397..12587)
                     /gene="dltD"
                     /locus_tag="KPB88_002522"
     CDS             complement(11397..12587)
                     /gene="dltD"
                     /locus_tag="KPB88_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG0054790.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         4180 a         2272 c         1257 g         5089 t
ORIGIN      
        1 tcatttttgc ttaaaaccta taatcctaat tataaattta ttcacctata ttttttctaa
       61 atcaactact gttcttccaa catgttttcc tgccattatt gaatctatac tatcatttaa
      121 ctcttccaat gatactacat ttaccatatc atctaaatca tctaatttcc attcatttga
      181 taataaatcc catattttta atcttaagtt cattgggcaa tccacagaat ctataccata
      241 taaagtaatt ccctttaata taaatggata tactgaactt gtaaaatcaa ctcctcctgc
      301 attaccacaa gttgttactg ctccacaata attagttgtc tttattgctg ttgctagagt
      361 atttcctcct actgtatcta ttactccagc ccatcttggt ctaagcagag gtcttcctga
      421 gttatcatcc acttctgacc tatgtataac atctttaaca cctaagttta gtaatacttc
      481 tttttgttct tctaattttc ctgttgaaac tacaactgaa taacctaatt taatcaatat
      541 tttagttgca attacagcaa ctcctccagt accaccagtg actaatacat ctcccatatt
      601 cttgtctaca gaatctataa atttatacac agataaagct gctgtaaacc ctgctgtacc
      661 ataatccatt gcttctttta gtgaaagatt tttaggaagt ttaactaccc aactagaagg
      721 aactcttatg tattctgaaa aaccaccaga agtattcatt cccaagtcat aacctgttac
      781 taaaacttct tctccaattt taaaatcatc acaactacta gattctacta taccagatgc
      841 atcaattcct ggtgtatgag gatactttct cgtaacaccc ttattaccat ttgcagaaag
      901 tgcatccttg taatttaaag aagaatattt tacttttatt aaaacttcgc cttctggcaa
      961 tgtatccact tctctttcaa ctatatttct tctaaaattt ccttcttctt ctgtaatata
     1021 tagtgattta aattttttat tcataaattt cctcccaata catttagttt tatgttaaac
     1081 taattatagt ttataaatag ttttatgtca aactaattta aatagattaa agattggaga
     1141 ataatttatg gaagataaat atattgtaca tttcataagt aaaacaaagg ctagtatgat
     1201 taaattcatt gaaaataaac tcagtaaaaa tggtttaggt gagttaatac ctactcatgg
     1261 aaatatttta actgcacttt atgaaaataa tggaatactt acaatgaaag aaattgcagc
     1321 taaaatagga aaagataaat ccactgttac agtgttggtt aataaactta taaacctagg
     1381 atatttagaa agacaaaaat gtacaaatga caaacgtatc acgtatataa agcttacaaa
     1441 aaaggcactg cttatagaag atacttttaa tagcatctca acacaagtta aagagacagc
     1501 ataccataat attacagaaa atgaaaaaca agaatttctt agacttttaa aaaaaattaa
     1561 tgataatttt aaaaatgctg actaaattga gcataattat atctcctatt ggaataataa
     1621 ataaaaagaa tcaaatattg taaaaattta ttgtgataga gaggtagaat atgaatttaa
     1681 aagatagtaa aactaaagaa aatttattaa gagcttttgc aggtgagagt caagctagaa
     1741 atagatacaa tatttctgca tctgtagcaa aaaaagaagg attatatata atagagtctt
     1801 tatttaatta cactgctgac caagaaaaat cacatgcaaa acaattcttt tctaaattaa
     1861 aagaatttgc tggtgaaaat ataagtatat caggagcata ccctgtagat ttatatgaca
     1921 agacagcaca acaccttaaa gctgcacaac ataatgagct tcaagagtgg gatgatgtat
     1981 ataaaaactt tggaaaaaca gcacgtgaag aaggatttga atctatagct aagttatttg
     2041 aaaatatagc tgaaatagaa aaagttcatg gtgatagatt tggtagatat gctactgatt
     2101 tagaagaagg tactttattc agaagaaatg aagatataca atggatatgt acacattgtg
     2161 gccatataca tataggtaaa gaagctccaa aagcttgtcc tgtatgtctt catccacaag
     2221 gatatttctt agattttaaa aactcattat ttgaataaat taaggcaata attctattta
     2281 aacaatttac tatatttata tcaagtattt ttataaatac tgtattatct taataacaat
     2341 aaaatgaacc attgatatta ctataagtag aaaatgtagt ttaaatacaa tttatggtag
     2401 ttcatcatgg ttcattttat tgtttactaa tccctataac ctacttaaat ctatctttaa
     2461 cttttacaca ctcacataaa atctcatctt taaactaaat atttaaataa agtgtatttt
     2521 aagtagatta ttataataca ttatataaga taatacacta tgataacttt atacttatat
     2581 catttatttc ttttgtcaag tcaatctcaa taatcttaac tacatctttt tcaaacttta
     2641 cttttttata atccagacat cctactacga atggaactcc aaactttcta aacaaataaa
     2701 tcttcccact ttcattgtat attgatgtaa aattttcttc tattatattc actatcaatt
     2761 ttaactcttc aaaagttatt atagatttaa ctaaaaactt ctctattatg ctcaatataa
     2821 gcagttcttt tttacctatg ctttgctctt ttttatataa attttgaaat atttctttgg
     2881 tataattaca tataacttct ttactgagaa taaaatcaat gtcaaattct ttattaatat
     2941 cctttttcat aaacatattt gcaatatcat caagagaagt ctcttctttc atagacaata
     3001 ttaactctat tctttctagt attttatctc ttggaaagaa agtctcttga cctgtaaaag
     3061 ctgatttttt tataaaccat tcctctggta ttatgttttt tcttttccat ctatataatt
     3121 gaccataaga tatattagtt ttgctcaata aatctttctt agatattaat tcttccataa
     3181 cttttctagc ccacctttat actgtttttt acagtagaat tatctgaaac tgataaatta
     3241 tcactgtatt caatatttcc aacatcacaa ttatctacta gagatatgct tttacctcgt
     3301 atatctttta ttgatgcatc ttctaattca atagtatctc cctctatcaa attacattta
     3361 aactttcctg aaaaaatatt aatgatttta attaatgaaa aagcatcttt gcttcttcct
     3421 atttttatat tagttgcccc tatctcttta caataagaac ctcctcctga ataaacatat
     3481 atattttctc catttaaaag accatcacat tttattgctc catatacctt tatatcttca
     3541 cattctatat ttgatttatt tctcatttcg ccatagattt ttatatcttg tgcatacata
     3601 ttttcattta cacttaattg tcctcttatt gtaacattat tacatttaag tttgccatcc
     3661 actttctgtt gagcagttat aactaagtca tctatttctg catctccctc tatattaagt
     3721 tgaccattca ctcttacacg ctctgcttta agattgcctc ttataatagt ttttccgtta
     3781 acaacaaaat tttttatatt gatgtcacct tcaaattttg catctccaag gacttttact
     3841 tcgttacttt taacatttcc ttttgattta gaacttccca caatttttat attttcatac
     3901 tcaccactac ttataaagcc ttctccaagt atttttatag accctttttc catattatta
     3961 cctcatttta aattttattt ttaaacctac ttattactta tccttcttta attttatttt
     4021 ttcacttata ttctttattt caccaactat tgaatcttca tcaatctcta tactttcact
     4081 gtactccaaa ctttctacat aacatccctt gcctatttta atattttttc ctcttacaac
     4141 tttgacttca ctattttcta agcttatttc atctgcttct ataatatttg caaacaattt
     4201 actttctgta tactttctaa ttgataatag acctaaactt attaaatttg aagacacttt
     4261 ttcaaagttg atatgctttt tttctagcat acgaaatttt ttgcaataat ctccatgccc
     4321 cttttttata tttacactac ttgctccaac ttcattaaaa ttagaagttc catttaaact
     4381 aatattaact tcttcacaat tcaaaaatcc attacactcc aagattccac ttaaattgat
     4441 attttcacaa tctatattct catttgcttt taaatatcct tctccatgaa taatatccga
     4501 ttttatactt ccttctacat taataattcc actagtatca atttcaacag aattaatatt
     4561 tcctgtacat ctaagaagtc ccgatatctc aacttttgat gccagtatgt ttccttctac
     4621 tttacttaaa ccagatgtaa aaaattcatt acattctgtt gtcccaaaaa cttttgctga
     4681 accagaagaa tcaaatttac tacattttac acttccttct attttgcctg ttcctgatat
     4741 attaacatta tcaaaaattc cacctttaca ttttccaaca ccatcaaatc ttagtgaact
     4801 taattcttca ttcataatga tatcctttct ttaccgtatt ttatctttaa acctaatata
     4861 acaaaacatt gttacgttgt caagtatgat aataatactt aataaagcaa ctatcattta
     4921 ttttctataa ataggcataa aaataatagg gaaattcttt atttaaacta taaatctaat
     4981 ttcttagatt gtggtttaaa taaaatatat ccctattact caatctttat atattcattg
     5041 tataaacttc taaaatataa atctttaaat tctactgtca atacttttta tacactgttt
     5101 gtgctatcat ctcacacacc taaaggagtg ggctttccgc actattctgt aacctacaag
     5161 acactatctt cttctatcta ttgataaatt tttctattac atcagctagt ctctccatat
     5221 cagttggata aacttcacct atgctaccta ttctaaatgt atctatatca gtcaatttac
     5281 ctggatagat aacaaatcca ttatctttta aataagtata aaattccata aattcaaact
     5341 ttgcattttt aggatataag aaagttgtta ttacaggtga ttgagcatta tcattcacca
     5401 aagtatcaaa tcctaaagat tttaatcttg aagctattgt aaactgattt tctttatatc
     5461 tagcatatct tttttcaaca gaaccttctt cttccaattc taataaagct tgataaaaag
     5521 ctcttacaac atgtgtaggt gaggtaaatc tccatttacc attatttttc tccatagtct
     5581 cccattgcgc atacacatca agagataatg atttagctat acctttacat ttagataatt
     5641 tctctttatt agctattata aaaccaaatc caggtactcc ttgtatacat ttgttagaac
     5701 tactaactaa aaaatctata ttaaaatctt caacatcaat ctcaattccc ccaaaactac
     5761 tcatagcatc aactatataa attttgttat attcttttgc aagtttccct acctcttgta
     5821 taggatttaa tctacctgta gtagtttcac agtgaaccat tgatatatgt gttatatcct
     5881 tgttttcttt taataaattt tctactacat ttaaatccac tgcttctata tctttaaatg
     5941 tgcaatcaac aaattctatg ttaagataat tacatatatc tttcattctc tttccatatg
     6001 ctccatttgc aattacaaga agtttaccat cttttgaaat agaacttcct attatagctt
     6061 ctactgaaaa agtcccactt ccttgcatta atacacttgt gtatttatct gtattttggg
     6121 ttgctagaga tactaatctt cttctaacat cttgtactaa gttattatat tcaacatccc
     6181 aagtacacca atcttttaac atagatgctc ttactgattt agtagtagat agtggtcctg
     6241 gtgtaagtaa tatgtaatca ttttctggta caaaatctga tttaatagtt tcattctcta
     6301 tttttattag tatatcttct aactcagcca tagtttctat tacaaaatgt gctcctgcct
     6361 ctttaaattt ctttgaaact atactcatct tagcttttaa ttcttcctta tccatgttct
     6421 ctacttcttc ttgtgtaagc cctaactcac ttgaaccttt tattacagca acactccaca
     6481 ttcctgcatt tacgccttct tttacatcac ttattgtatc tcctactttt accatagaac
     6541 tcataggaga cacccctaaa gcttcagcat ttttatagca catccaagga tacggtcttc
     6601 cttgagatac ttctgatggt gttacaagaa aatcgggtga gtatcccttc tttgctgcat
     6661 ttggttcaac tatgttcatc atctctcttg tataacccgt tgtagaacct atttttaatc
     6721 cattttttct caacttttca atagtttcaa caacatgagg aattggagtc gtataatctt
     6781 ctaatgtctc aaataacatt ggctcaaatt cagcataaag ttcatttaca tcatcttcag
     6841 ttggcacttt accaaactta tctgaccaaa gattttttat cctatccatt tcacacattt
     6901 ctcttatatg gtctattttt aattttccca taggttttct tgcttcttcc attgttacat
     6961 ctatacctct tcttttaaat atctctataa atacattaag aggtgcaaaa catccataat
     7021 ctactgttgt tccagcccaa tcgaatacta ctgcttttat tttttctcca tatatctttt
     7081 tcatatttat agcctccata gattcttttt agaatttttg tgtttttaat tgtttttact
     7141 tttaaagtat aactttcata tgttaattat acatcatatt taggttaaat atatattaaa
     7201 tttctgtttt tagttgcatt tagttgtttt tattttgtat cacattccca taaaatccta
     7261 agttacaatc taaaatttac tttctaatct taatctatga actgactata ttttatgaat
     7321 ttagttgtat tgagcatagt tgtattatat aaaactaagg gtttattttg cttctatcca
     7381 tgttatattt atctcattta acttatcctt ccattcattc ggaggttcta catcacaaat
     7441 aacaaaatct aattcctcta aatctgctat cttatacata ctagcaattc ctattttaga
     7501 actatcacat aatacaaaac tttcctttga attttttata tattcttttg acaacaatgc
     7561 cttattttgg tctatactac taattccaaa actatcaagt ataccttcac tagatataaa
     7621 agctttatct acgtataagt ttttcatagt atctattgat attggacatg ctgtcctttg
     7681 gtgttttaaa tttacttttc caccaataaa tataacttct ccatcaaaca aaccttgatt
     7741 ctcataagaa atcaattttg atagaactgg ataagagttt gtaataattt ttaaattgtt
     7801 tacagatatt atataatcta caatctgcaa attggtagta ccttcatcta tgataattga
     7861 ctcattgtca tttacaaact tagctgcata tttagcaata ctttgctttt tatctaggtt
     7921 agtattgttt ctctttatat gttctggttc aatcactcca taatttattt ttatagcacc
     7981 accatatacc ttttttaatt tattttcatt ttctaattct tccaaatatc ttcttattgt
     8041 ttctgatgat acttctaatc tttttactaa ttctttagtg tttaccttcc cttttgaatc
     8101 taataattta agtatttctt tttttctatc tatcgaattt aaagacattc attttcctcc
     8161 taaatataat atatttttct cctataatat tacaatgata cctaagattt atgaaatcat
     8221 atttatgtat taaatttttt acttaattaa agttattttc aaaagacttt gtattatcat
     8281 taaaaaagtc catcaaatat tagtatcaga tggactttgt aaaattttat tttataaatt
     8341 aatatttaaa gtctaactac taatttattt tcttgtctcc aagaatttta ctaaattatt
     8401 tacagtagac atatcttttc tctctaaatc agttggttga agctctatcc ctaatctatt
     8461 ttcaatttca agtaaaactt caattatagc taatgagtct aaaagttccg tctcaaataa
     8521 attaaggtct aaatcttctc ttatctcatc agtccctaat acatcttcaa atatttctat
     8581 aactgtttct tgcataaatt ttcctcctta aaattaagct aaataacctg agaatatcag
     8641 caatccaaaa cacacaatat ggaatgtaat gaaaatctgc actctctcga accatttatc
     8701 ttttttatgt tttttataaa atttagattt tcgctgatat atatcagtta acacaagtgc
     8761 caatccttgg tatacaccat aagcaacata ataccaagtt agaccatgcc aaaatcccat
     8821 tgttatcatg gttatcatct gagccacatg agcagcagta gtccttttct taaatctttt
     8881 ctttctcatt gatgacataa caaatctaga aaaaatataa tctccaaacc atcttgaaag
     8941 acttatatgc catcttgtcc agaactcttt catatctttg ctgataaatg gcttgtcaaa
     9001 gtttataggt acttgtattc caaatatgta ccctgtacct atagcaaata aactatatcc
     9061 tgcaaaatca aagaacaggt ataaactata tgcgtacata taacttaaaa tatttataaa
     9121 actcatatct ttaggtattc ttgataccca atatgtattt atcaaaaatg ctataacaaa
     9181 cttataacct actcccatga caatattttt tattcctggc agtaagtatt cttctatata
     9241 ctcttttcta gagatttgtt tttccaagtc ctgttcaaat cttcttgacc tatcaatagg
     9301 tccagaactt aaagttggaa aaaataacat aaaataaagc atctttgaaa ttttaacttc
     9361 ttttatagct ccatcataaa tttcaataac catttgtata gttctgaaat ttaagtatga
     9421 tattccaata aatccaatta ttccaaaaga agtaactgga gatattttat ttattattat
     9481 tgggagcatt gatgcaaaca agaatcccca atatatgtat ttatttttag tttttcttct
     9541 tacatattca tatcctttaa ctattattac ttcaagaata ataaatatta ccaagtactt
     9601 tagttggaca tcaattccta ctattaagaa tatcataaac aaacttgcca acataccata
     9661 atactttata ttcttgccca ttagacctaa aattactgct ggtattgatg ttaataataa
     9721 tatataaaga taaaaataat ctccatattg tgaaaaaatc attaaatttc ctccataagt
     9781 ttctttctgt ctattttacc attgatattt gttggaaact cagagattat tgaaatattt
     9841 cttggaatca tatatgaagg tatatacttt ccaagttcct tttttattat catcccattt
     9901 ttaatattac ttaaatcatt cttttcattt aactcaacaa taccttttaa gtaagcaatt
     9961 ttttcgtcct tatatactgg taaaactact gcatttttta cattatgtac ttttctcaaa
    10021 ttattttcta tgtcttctat ttcaattcta aatccattta atttaatctg aaaatctttt
    10081 cttccacaat aatatatatt tccatctaaa agatatccca tatctccagt tttataagct
    10141 ctccacttta caccatctat ttcatcatag aaaaatactt catcagtctt ttctttatta
    10201 ttgaagtaac ctttagaaac tgatggtcca attattataa tttcaccctt ttcattttct
    10261 ttaagctcat ttccatcttc atcaagtatt tttattttac aatttgacat agggtatcca
    10321 actggaagac ttttttcatc atctatagcc ttttgagtca tatcatttac acttacacca
    10381 acagtagcct cggttggtcc atatccattg ataattcttg tgtttggaaa tctactcata
    10441 agttctttag taagattttt tgaaagtgct tctcctatga atatcattga ctcaaggtta
    10501 ggaagcattt tactattaaa ctctttttca gttatacata ctcctgcaaa agaaggagtt
    10561 gacacccata cagctatatc tgagatacta aattgtctaa atagttcttt atagtctgct
    10621 aatacatctt ttgatattga aaataatgta gctccatgga taagaccagg gtatattgtt
    10681 gtaactgaaa ggtcaaaaga atatgctggt tggttcatta ttactttttc acttccatct
    10741 atgtttagat atgggcttat ccaatctgaa aagctatcta aattgtttga acttatttga
    10801 accccttttg gctttcctgt acttcctgaa gtaaatagta tatatgcatt ttcatcatct
    10861 tttacccagt tttctttatc taatgatttt ccctgatatt catttattat ataatttaat
    10921 ttatccatat caattacatt gatatctcca aaatttcttt catcactaaa gttaaataat
    10981 acctttggtt ttatctcttt agtgacttca aaaactctat caattggaaa actaatatca
    11041 agaggcacat acgctctacc tgattttaaa gctcctatca tacatgccat tatcatgttt
    11101 tctttatttc cataaattac tataggagta tcttcttctt tgtatacatc ctttagaaat
    11161 actgatatag catctgaata ttcatttaaa tctttataag aaagtttatc tccattacac
    11221 attagtgctg ttctatctgt atttgaatat ttttttattc cttcaataat tttcatataa
    11281 gaatgctcct ttaaaatccg ttataaataa atggcaattc tttaaatgga gtaaatgtta
    11341 gaactcctat cgccaatatt ataaccatag ttaaacaaat actagttaac aaatctttat
    11401 tgttctttaa atattttaaa taatctctca cagacatcta cccatccttt cgtaccaaga
    11461 tgcattacat cccttaaata atacttatct gaccctttat ctttaaggtt tagaacttca
    11521 aagcctttgc tttctgctat tttttgtgct ttatcgtagt actgatttct ttctttttca
    11581 gaaataccag tcaaattata gaatttatcc atacttggga ttaatacaac aactggctta
    11641 atacctaagt cagtacatac atttagcatc agtttataat tttcaaactc cttagatgtt
    11701 aataaattta catctttgtc tcttcctttg tattgctcta taccatcttt aaaatgctct
    11761 ttataatagt acttatcaat atttagtgga tttttaccaa ctcttttttt agcatcttca
    11821 atggctttct ttctctcata tgaccaattt attggtcttt ttctttttgt tgctctcttt
    11881 ttatctagct taattaatcg tttataaaga ataccctttt ctttaagagc tatacaatac
    11941 tttcttcctt gaaaataagg ctctaaaagc accttttcag ctttttcagg aaatgtctta
    12001 ggctcatata gcttagcata taatgcttct gctttatact cgtcacttcc ccataataac
    12061 ttacttgatt tttttgcata ttcaatctta ttttgtttag atatttttgg attatctaaa
    12121 aatcgataaa attgtattgg tgaaaatcta gactgataat ggtgacttgt gactccgtct
    12181 ttttccataa accattgcat agaaattaac aagacaactt tcttattatc aatattagga
    12241 ttcatacttc ctaatattgc agcatcttgt aatgtctgag tataggctct cccaattgca
    12301 aacaccttat ttttactcct atttgtatta aaatagtact ttggatgttg ttttgttgaa
    12361 ttactaagtt ctgatgaccc caaaatcata atatcctttt ctcttaaaaa atgattattt
    12421 gctgtaaccc ctttatcttt tatatcactt aaagtatcat ccataattgg aagtagattt
    12481 ttctccctca aaagttcatc agttttagaa tcaagaaatt tgtctaaccc aaataaaaat
    12541 actacgccta ttataaaagg tgtaataaag tatatcaatt ttctcatatt ttctctcctc
    12601 taaaaatatt caaatttatg ttattattaa tcatattagt tttgatatgt atattcaact
    12661 acaaaattgt aataaaatcg tcaaaacttt ttttgatact gttacaaaat tgtaaaaaaa
    12721 aagaaaaaaa cgccaaaaat aaatttcttt ttagcacttt ttcatactta taatttgaca
    12781 ttttaacact tggttaag
//
LOCUS       DAELJO010000113        12547 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELJO010000113 DAELJO010000000
VERSION     DAELJO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.113"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(215..1882)
                     /locus_tag="KPB88_002523"
     CDS             complement(215..1882)
                     /locus_tag="KPB88_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG0054791.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1956..2711)
                     /locus_tag="KPB88_002524"
     CDS             complement(1956..2711)
                     /locus_tag="KPB88_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG0054792.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2721..3257)
                     /gene="lepB"
                     /locus_tag="KPB88_002525"
     CDS             complement(2721..3257)
                     /gene="lepB"
                     /locus_tag="KPB88_002525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0054793.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3423..3959)
                     /gene="lepB"
                     /locus_tag="KPB88_002526"
     CDS             complement(3423..3959)
                     /gene="lepB"
                     /locus_tag="KPB88_002526"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0054794.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4059..5369)
                     /locus_tag="KPB88_002527"
     CDS             complement(4059..5369)
                     /locus_tag="KPB88_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG0054795.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYLDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5448..6164)
                     /locus_tag="KPB88_002528"
     CDS             complement(5448..6164)
                     /locus_tag="KPB88_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG0054796.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6409..7680
                     /gene="sleC"
                     /locus_tag="KPB88_002529"
     CDS             6409..7680
                     /gene="sleC"
                     /locus_tag="KPB88_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG0054797.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8188..8844)
                     /locus_tag="KPB88_002530"
     CDS             complement(8188..8844)
                     /locus_tag="KPB88_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054798.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPGGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDDM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10117..10527)
                     /locus_tag="KPB88_002531"
     CDS             complement(10117..10527)
                     /locus_tag="KPB88_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054799.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10743..12125)
                     /locus_tag="KPB88_002532"
     CDS             complement(10743..12125)
                     /locus_tag="KPB88_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0054800.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12136..12495)
                     /locus_tag="KPB88_002533"
     CDS             complement(12136..12495)
                     /locus_tag="KPB88_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0054801.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
BASE COUNT         4194 a         2291 c         1240 g         4822 t
ORIGIN      
        1 cataaactaa caaaatatta aacaaaaaag taaaatttaa aataaaaagc ttgcttcata
       61 caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct gtatcaattt
      121 aattctccac tttacactta atatacttaa cataaaccaa taacactact tgctatgcca
      181 tttaatcttc ataaaacaat ctataataca cacattaatc aactatttta cttcctccaa
      241 taaattccct aagaattgaa atacctggta tgtcagaagt atcgtccaat tcaacaaaat
      301 actgcaaaaa ctttagtaat ctagtagctt caatatgtgc aggattatct tcctttatct
      361 cttccaacaa aggtttcgca aatcgtttta aaactgccaa ttcatataca caagcagagt
      421 taaatatagc atctgcctcc tcttggtacg ggaatatatt cttcttttct cctcttctaa
      481 ctgatgccca ttgtaaaata gtattatcag cactatatcc tctaaaatta tagtctctaa
      541 caattcttct tatcattctc aaatcagcag tagggattcg attatggtca tccaaattta
      601 tttgagtaag ggcacttatg tatatcttaa atttatcttc atctggtata gatgatgtta
      661 aaataggatt tagcccatgt ataccttcta atataattgg ctgatttgaa tctatcttta
      721 tctttttacc agtgtattct ctcttacctt ctttaaaatt gaatcttata tcatcaattt
      781 cttcaccggc taaaagtttc tttaagtcta agttaaactt ctctaaatct attgcataaa
      841 ttgattcaaa atcataattt ccatattcat ctaaaggagt atgttttcta tctataaaat
      901 aatcatctaa agaaattgaa ataggattta aattatttac tctaagatgt attgataatc
      961 tgtgagcaaa acttgttttt ccagatgaag atggagctgc tattaaaact actctcttat
     1021 tgccatcctt tatcatatcc gctatttggg ataacttctt ttcatgcaat gcttcaaatg
     1081 tccttattat atctccatat tcattgtttt ctattatttt attaagagat actaccttgt
     1141 caattcccat aagtcttgac cattcttctg cttcataata tacacttgat agcttaggtt
     1201 gaggtacaaa tttcatagga agagtcttat cttcttcact aggtcctaaa attataacgc
     1261 ctccattata aagcttaata tcaaatgatt ttatataacc agttgatggc aacatataac
     1321 cataaaaatg gtctatatag tttctacact tatatacttt agcactatca tactctttgt
     1381 atttcaaaag ctctgccttt tcgtacattt cattattttc aaagatagaa atagcttcat
     1441 ttcttgtaac ttcaattttt tctataacat aatcgttgtt tactatctct ttcatcttgt
     1501 tttttatatt ttcaacatct gcttctttaa gctttctatc tatatgaact tcacaatata
     1561 gcccatctga tagtgaatgt tctattgtaa ctctgctatc ccaaaagatt tctctacatg
     1621 ccattacaaa tataaatgac atgactctaa agtaaactct ctctccatct tcattagtag
     1681 tatctaaaaa atttatctca caatccttct taactctaca atttagctct tttagtttat
     1741 tgtctacaac tgcaagtgtt atataaccct tatgctcatc acaaaattct tttgctatat
     1801 ctcccaatct gattccaggg gaattttctt ctaatgaata ttccttatct tttccttcta
     1861 tttttaagtt tatttttttc acttcaacat ctccttcctt ttgatgaata taaattttaa
     1921 ttaatgaggc atatcaacaa gataaagaga ggtatttaac cctctcctac aatattattc
     1981 aatttactta tagtatcttc aatgtcttca taatttattt ctaatactaa atattctgca
     2041 tctaatgtta agacctcttt aatcccctct tcagataaaa ttcctcttcc tataccaaca
     2101 tgtgtatcat tgataccatc attatcactt aagtgtatca gtcttatttt atcctttaag
     2161 ttctttacat aagcatcctc atcaattaaa cagtgtccag tatcgtaaca aaaacaaatc
     2221 ttagaatttt taatcctctt aaatatatat tcaaaatcat aagatatatt tccaatattt
     2281 gaaaaatccc caccttttga ataagtattc tctatacata catatgaatt tgtatttaat
     2341 ttatctaaaa aatccacact atttcctaaa tatttgtctc tattctttct aactctattt
     2401 gtcataacat atcccaaatg aaggttaaaa taccttaaat caaatccttt tagttcaaat
     2461 tctatcttct caataaaatt tatccatgaa tttcgtatat actctatgtt ttcgcaagta
     2521 tttaattcca taggcaagtg tatccctata gataatccta acttagaaat tctctcttta
     2581 tatttacata actcactaca ttcagatata ttgtcaattc caatctctaa atgagttatt
     2641 tgctttatac tctcacaaat gtttaaagcc tcctcaatat taaacaataa ggacgctata
     2701 ccaactgcca tacaaatcac ctactcaaaa atacctatat ctgtaaaagg aaatactctt
     2761 aaaataactt ttcctttaat attttcttca tctactaatc ccacttcttt atatctactg
     2821 tctaaactaa cttctctatt gtctcccatc gcaaatactt ttcccttagg aatcaccata
     2881 tcaatatcgc cttcagtata attatcatgt atatatgaaa cttcatttaa taacttacca
     2941 tttatataaa ccttagaatc cttaattttt agatggtcac caggaacacc tataaccctt
     3001 tttacaagct cttttttcct tccattctct tgtaataaat cagtcttaaa tatcattata
     3061 tctccacatt ttggttctga taacttatat gtcatcctgt ttgctaccaa ataatcatgt
     3121 gatttaagtg ttgggtacat tgattctcca tttactaatg ttggttttat aaaacgagtt
     3181 ataataaatg caaaaaaaag tgcagtaata attattttta tccactctac aatctcttct
     3241 ttaatagttt cattcattca caaattctcc tttatagtta tattatcttt attgataatg
     3301 tctcttaaac actatctttt agataagtct actaaataag gtgttgtctt ttttatatag
     3361 acaactattg tgtatggtct ggtgatttca gctaatcagc gattgctcca aattttatat
     3421 gtttagtcaa tagttcctat cttagaaaat ggatacaatc tgactagaac ctttcctaaa
     3481 atggtatgct cgtcgaccaa tccaacctca tcgtatctac tatctaaact tttttctcta
     3541 ttatctccca ttgcaaataa ttttccttct ggaactacga tatcaatatc tccatcagta
     3601 cgattattat gtatatagga agtctcatct aacaacttat catttacata taccttggag
     3661 tcttgtattt ttacatggtc accaggaacc ccgataactc tttttactaa atcttttttc
     3721 tttccatttt cctctgttaa gtcggttttg aatactatta tatctttgta ttttggctct
     3781 cctaccttgt acgcaattct gttaactatc aaataatcac gttcaactaa tgtaggatac
     3841 attgattctc ctttgactat tgttggcact ataaaacgag ttattgcaaa tgccaaaaca
     3901 agagctatga caatcacttt tatccattct acaacttctt ttttaactgc ttcacccact
     3961 aaaatcgtct cctctgtgtt aaatattaag tcaatctaca gcaaaataat ctaccataaa
     4021 cctacaatat aaatattata cttctttttt ctaatagttt aaaatacttt tctaagacct
     4081 ttaggataat gattcttaat tacaccagaa gattcttttc cccaacctaa aggtattcct
     4141 tcaaccgaaa ccaaaaccca tcctctacta tcattagaat ttacaacatc cccttttaaa
     4201 tatttaagaa ttgattcact ctgtacactg aagttttcaa cattttttac atcttcaact
     4261 tttaagtaat gagatagtgc atgagaaggc tcaaatctat tcttctttaa aacccctaaa
     4321 tgcaaaccat atctcaatac ttttagtttt ttcgtatcta gatatatttc aggaagtagg
     4381 tacaaatttt ctcctcttaa ataaaattta ttttttttat tactataaaa atcaaccttt
     4441 aaaaatttct tttcaaactc cctataatcc tttatttctt tatctaaatt ttttattttt
     4501 aattctttga cattacattc ttcttcttct aattttttta ttttggcaac aaaatgacct
     4561 tctcccttta tcttatgagg ccataatctt tccatttcaa taagttctgc atgtggatat
     4621 tcactgataa actcatttat aacatgctca ttttcctccc ttgaaaaagt acaagttgag
     4681 taaattaaaa tgccatcctt ctttaacatt ttatatccat ctcttataat ctctttttgg
     4741 atggattgac attctactac ctttgcataa ctccaatctg atatggcaac ctcatccttc
     4801 ctaaacatac cttgcccaga acaaggtgca tctataataa ccttatcaaa atacccatta
     4861 aacgtctttc ttaagctatt agaatcggtg tttgttataa tacaatttct cgctccaaat
     4921 cgctctaaat tttcccctag agccttaatt ctactagcat taatttcatt agaaaccaat
     4981 aaacctgtat cgtttagctt agatagtata tatgtagatt ttccaccagg agcagcacac
     5041 atatcgagaa ctttttcacc ctctttaata tctgcctttg gtattacact cattgcagaa
     5101 ggctcttgca aatagtatgc tccagcctca tgcagtggat ttctaccagg tctgtcaatt
     5161 ttatcatcat aataaaaacc ttcttctgcc catggtattt gattcaaatc aaacaactcc
     5221 aaatcaaata attttttctt gtcaattttt aaagtattta ttctaagccc agtagtctta
     5281 ctttcctcat aactttttat aaaatcatca tattcatctt ttagtatttc ttcaatatct
     5341 tctaaaaatt cctttggtaa ttctgtcaaa tctataatcc cccttcaatc tgctttactt
     5401 gtgtgtcatt tattatactt taattattta tgtctttcct ttaaatttta tttcttcttc
     5461 tttttcctaa gattaccgaa aaacactttt ttagagtgat tcatatattt ctcaagttta
     5521 tcaagattta tcttccacaa aattattaca taaactataa gtaatataat agcccatcta
     5581 tattctatca tcaatatgaa atcaaaaact ccatgtaata aaattggaat taacactgcc
     5641 ataaacaaat actcacgtct tttattttta tttccctcaa atttatactt agaaatatag
     5701 tagcccatag taattgcaaa cattacatga gctggtatag agataactgc cctattaata
     5761 ccaacttgca gtgctagatt tctactttca gaaaaaatat aaaccatatt ttcaattgtt
     5821 gcaaatccta aagctaaaaa tacagagtaa ataataccat ctaacttttc agtaaagtgt
     5881 ttttctttaa ttagggcagg aatcagtatc agagctttta caccttcttc tacaagtcct
     5941 gcaactacaa atgatacata tattagttct gaataccctt caaatacatt aaacttcatc
     6001 agtaaatttt ctaaaagtat aattataaca cttagaaaag cacctattaa aaaaaattta
     6061 attagatgga ttaggggttc tctacaatat ctatctttag accttatcca aaacatccct
     6121 atcaatatag gaactattgc taataaaaat aaatctagct tcatagatta ttttcctttc
     6181 aaaatttttg atttatttat gatttatatc atctacttat ttttaactta ttcgtgtttt
     6241 aaatacatac taaaatatac attaaattat tttaaattat ttaaatacaa atatactcaa
     6301 atactcaaaa aaatttaaat ttatatctat attatttaac aaatttcaag ctatattcat
     6361 atcttgaaat ataagggaac taaattcatt taaagaaagg gtgatttgat gcaagatggt
     6421 ttcttaacag taagcataat tgatgctact aacaatagac ctattcaaaa tgcagtagta
     6481 aacatatatt caatgtctaa tggtagccaa agctcatcaa ctttatacca aaacctaaga
     6541 agtaatgaat ctggtcaagt gactggttta gtattaccag caccagatgt agattactct
     6601 ctacaaccat ctgatgttag accttatagc cagtatatag tggaagccat agcagatgga
     6661 tatgagacag ttgtcataga aggaacacag ttgttagcta caattgaagc aagacaagga
     6721 gttcccatgt ctcctagaac aagaagtaag cgttctttct caagacaaag tgagttgatt
     6781 tttgacatag gagaacatac tctatatggt acttatcctc ctaagattcc agaaagtaac
     6841 ttaaaaccat taccacctcc tactggtttc gttgtattag ataaccctgt agtgcctgaa
     6901 tttatagtag ttcatgatgg tttgccagaa gattcatcag ctccaaacta ttggattcca
     6961 tttaaagagt acattaaaaa catagcgtca tctgaaatat attcaacttg gcctgaacag
     7021 acaatttatg caaatgtaat agctataata tcatttactt taaatagagt ctttactgaa
     7081 tggtatagaa ataaaggata taactttaca ataacttcaa caacagcata cgaccataag
     7141 tttataaata atagaaactt atttgaacca attaacgtag ttgtagatgc tatttttaat
     7201 acttttataa aaagacctcc tacatcaaga cagccactat tagcacaata ttgtgatgga
     7261 caaaaatccc aatgtcctga ccaaatgact caatggggca gtaaagactt aggtgaccaa
     7321 ggatatgact atgagagtat actaagatat ttctatggag atgagatagt atttgaaaga
     7381 gcacctatag taagtggtgt accagtgtca ttccctggta ctacattaca agttggttct
     7441 tctggacagt atgttagaac catacaaaat cagttaaatg ctatatctaa ttcttatcca
     7501 gctgtaccaa aagttataga ggatggtatc tatggaacag atacagaaaa cgctgttaag
     7561 atattccaag gaatctttgg tttacctcaa agcggagtag tagattttaa aacatggtat
     7621 gaaatatcca gagtttatgt agctacaact agaatagctt ctttaaatcc tttaatttaa
     7681 agcttgattt agaacataaa ctcaaattat tgaataaaat tattaattta aaataaaaat
     7741 ggctgttatg aactaatata taccataagt attacttata agtattgtag tcttaacagc
     7801 cattttttag ttataacttg tttttttaga atcttaaaac tttaaatggg ggattttaag
     7861 cttcttaaaa acagtgctta gcaacttaga tttctttaaa atattatatt tatttttaag
     7921 tttaataaaa tgctattcta acgaaaaacc tacttattat ttaaaatctc acttattatt
     7981 taaaatgcta cttctcactt agagtattac tccttattat ttaaaacact atctatcact
     8041 tagagtatta cttcttatta tttaaaacac tatccatcac ttagaatatt acttcttata
     8101 taaattatta tttattataa atacaaataa tattaattta aaaagttatt cctaatttaa
     8161 ataatactac tatcttcatc agtgaaccta aactgcaact ttagacttat tagctaaagc
     8221 caatattaga cctaaagcta tagatgcaaa tgcagaagca attactctat actcagcagg
     8281 gaataagaat acaattgtat gacatggtat ccagaaaaat ggtatagttt ttaaaacact
     8341 aaaaccaagg taagcattcc agtccacaat atttaccaca tctttaagtt ttaccttaat
     8401 cccctttcca acctcatatt ttttatctat aaaacagtcc ataaccttat ggaataaaaa
     8461 tacagttggt gacattagta tattcatagt tgcactctta aatagagcat ttgcaaaagc
     8521 tattccctca aatggaagca tatcatccac ttgtgcttga cccacacctg atgagtagat
     8581 tgacattcca tatgtggtaa ctacacctat tattccccaa actattgctc tataaaatac
     8641 tcctcctggt attttccaat ccttatacaa aattctagca ccaagtaaat ctcccattgt
     8701 agctaataca gcaaacttta aaaaacctcc aatatatgga tgtgcacctg ttgcttccat
     8761 aaatacttct ctactacttg gtattgctaa gacagcaata atacaaataa gaactccact
     8821 ccatataaaa tcgccttttt tcatatttat aaatactatt taatatataa atagttctcc
     8881 tttctattaa agatttattt aatttacaat ctatctacaa ttatttatta atcacttatt
     8941 attagatatt cattttccat atttaaattt attctatccc tttatctctt tattttctta
     9001 ctactctatc caccattatc tatatattta ttttacgatt actctttcac cctaaccctt
     9061 taattatcta tttttttctg atattcttat ctaccatcat ccatatattt attttacgat
     9121 tactctttca ccctaaccct ttaattatct attttttctg atattcttat ctaccattat
     9181 ctatatattt attttacgat tactctttca ccctaaccct ttaactatct attttttctt
     9241 actattctta tccacatcat ccatatattt attttacgat tactctttca ctctaaccct
     9301 ttaactatct attttttctg atattcttat ccaccatcat ccatatattt attttacgat
     9361 tactctttta ccctaaccct ttaactatct atttttttct ggtattctta tctactatta
     9421 tctgtatatt tattttatga ttactctttt aacctacccc ctatacttta tagtaatatg
     9481 tttttaacct ttttcattat tcccaatatg ctttataact acaaatactc tcccccctta
     9541 aaaataataa taaaattaat caataaattt ataatcatat tattactcta tgtattaagc
     9601 aattactatg ccaataaact ttgatttttt taaacaaatc ttaaaaatcc taaaatgcta
     9661 attttcttta acttttaatg aatctaatag ataacattta attttcgtta tatttttcag
     9721 aatatttcta ttattatttt taagaaaaaa aaataatgtt aacatttctt aacatacatt
     9781 aaaaaatgtt aacattataa attgggttta atacttttac tttgactcga tttactttta
     9841 ttctacttct tactttattt ttaacttaga ctttattttt ttattactta tttgagtctt
     9901 atttatttta ttctcactcc atatgttcaa ttacatttgt ctaaattctt tgatactttt
     9961 attacataat tttaacaagc ttaagtgtta tttgggtagt gcagttgata attatttaaa
    10021 taatcatcaa ctgcatgttt atatcattac agtagcttaa tgtatttaat catacttact
    10081 taggattttg aaatatctta gagattatat ttaacattag tcaattgctg cataattgta
    10141 agcaattgaa atttgagacc catcttcatt ttctatagtg ataggcttca taatttttaa
    10201 tccttcagtt tttatctttt ctaattcttg ttccattttc ttaattgttt cctcaaggtc
    10261 tttttgactt acatctttga ctgcattagg gtctttagca tagtctttta attcttgaat
    10321 atgtttagca tactcttcat aagtataatg ctcaacacca tacttttcca attcagtagg
    10381 gtcagttatt cctgatttta ttagagattc aaatttaggt aaagcaatat tagatgaatc
    10441 atttccattt gcaaatactg ctccagttcc tcctagtacc attgcagata ccatcattgc
    10501 acttataagg gattttttta ttttcataac caatcttcct ttcttagaga tttatttggg
    10561 tagtgtagtt taaaatgact attgcaatag ttatcaacca cattcttaga ctatcacagt
    10621 agtcatgttt atgtcaagcc tttttaaaaa acttttcaat aaaaaatact atctcaaaac
    10681 aaaattaatt attttgagat agtattttaa ttctaaatct tttatattac taagatttat
    10741 ggttactcta tccatgttct atattgttct ttattaactg ctaacaaatt cccttttctt
    10801 gatatgacaa agcgattatc attatagcca ctttgctcaa ttgaaacttc tcctgaatca
    10861 gtataaagta catccttact attactagat ttatttacat acgtttcttc actattctca
    10921 gaattagtta ctttgctcat agatgattct tcatgctctc taaattcaat tacagatact
    10981 tttgccgtac taaaatgagt ctctccatca cttttagctt tactaacaag aatatcagat
    11041 tctactttgg gagaatataa atcagttgat ttactactta ttgaaaatcc aattttatca
    11101 ctttgttcat tttttgttaa accaaaagat tcaacctcat taatctcttt attttctgtt
    11161 gcaaaaacaa gacctgtccc acaaactaat acagttgaaa ctaaaaaagt aacataagtt
    11221 gattttttaa atttcataat attaactatt ctcttttcta tttttgtctt tccaaatcca
    11281 ctacatattg tcattgaaaa tggattattc tcctctgcta gacttattag tgtggttgca
    11341 taatcagccc tatattcttc accaagtata ctcataacct tttcatcaca actaaattct
    11401 atgtctttat ttagtaaaaa aaacatcacc cagactaagg gattaaacca atggacaaca
    11461 agagctacaa gcataaataa ctttaacaga ttatcaaagc tattaatatg aaccatttca
    11521 tgagttaaaa caaagtctaa agtttgatta ttagcataat tcatttgctt tggcaaaata
    11581 attcgtggtt ttaaaatacc agatgtaagt ggtgtcctag taaattcaga tatgcaaaca
    11641 ataaatcttc taaaacggct attcttacac atccattttt taatgaactc atgatttttt
    11701 atatgaattg aagttttaaa aagattaatt attttaaaat atctaaagca taaaagtatg
    11761 ctaaacataa tagtaactat aaaagacaaa atgcccaaca tttccaacat cataatattt
    11821 tcaatattag acattatttc aaattcgtca gaattgtttt tacctgtaga gtcactagct
    11881 ttcttaagag tataattttc tgttgatgta ttacccaatt ccacatcaaa agtcgattca
    11941 attatactat aaatactaaa accagaattt aaacttactg gtattagaag tttaaataaa
    12001 acaatcaacc acaataatac aaataccttt ttaggtaatt ttttaaataa acatgctctt
    12061 ataaaaataa ctattaaaag taatactcca gaagtaaaac tcatttctaa caatttaatg
    12121 aacataaatc ttacctcact ttaatttttc aacaatttgt ttaagttttg taatctcatc
    12181 acttgaaaga ttttctttat taataaaagc tgaaagaaat ttttctgaag accctccaaa
    12241 cattttatta attaaagttt ctgtttcctg tttttgtact tcctctttgc ttattaaagc
    12301 tttacaaaca aaattcggct caattctctc tattgcacct tttgacacta atttttttat
    12361 aacggtatac gtagtgtttc tattccatcc tgtttcttct tttaaaattt ttactaactc
    12421 tccagccttg ctttctcctc tctcccatag aacttccatt actttaagtt ctgaatcaaa
    12481 taattttatc tccatataaa atcgctcctc aaatatagtt atcacaatag tcattactat
    12541 aataaaa
//
LOCUS       DAELJO010000114        12401 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELJO010000114 DAELJO010000000
VERSION     DAELJO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.114"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            259..2064
                     /gene="lepA"
                     /locus_tag="KPB88_002534"
     CDS             259..2064
                     /gene="lepA"
                     /locus_tag="KPB88_002534"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG0054802.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            2213..3550
                     /locus_tag="KPB88_002535"
     CDS             2213..3550
                     /locus_tag="KPB88_002535"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG0054803.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            3783..5096
                     /locus_tag="KPB88_002536"
     CDS             3783..5096
                     /locus_tag="KPB88_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0054804.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            5268..6443
                     /locus_tag="KPB88_002537"
     CDS             5268..6443
                     /locus_tag="KPB88_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG0054805.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            6546..7565
                     /gene="hrcA"
                     /locus_tag="KPB88_002538"
     CDS             6546..7565
                     /gene="hrcA"
                     /locus_tag="KPB88_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG0054806.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            7595..8215
                     /gene="grpE"
                     /locus_tag="KPB88_002539"
     CDS             7595..8215
                     /gene="grpE"
                     /locus_tag="KPB88_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG0054807.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            8307..10154
                     /gene="dnaK"
                     /locus_tag="KPB88_002540"
     CDS             8307..10154
                     /gene="dnaK"
                     /locus_tag="KPB88_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG0054808.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            10365..11519
                     /gene="dnaJ"
                     /locus_tag="KPB88_002541"
     CDS             10365..11519
                     /gene="dnaJ"
                     /locus_tag="KPB88_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG0054809.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
BASE COUNT         5013 a         1349 c         2291 g         3748 t
ORIGIN      
        1 attaaaataa gccttatttt agttatatat ttttaatgta taaaataaaa tttcattaac
       61 tgtattttat aggatttact ttaagtttac gatattatat aatatttagt gttagacttg
      121 ataatccaca tataatgtgg ataattttgt ttagtgccaa ttagtttata ttcaaaaatt
      181 ttaaatttgg tatataaaga gtttttagat aatgtaaaat aataactata agaataatca
      241 agaggaggga attttaaggt ggacaataaa caaagtagaa ctagaaattt tagtataatt
      301 gcacatatag accatggaaa gtctacctta gctgatagat taatacaaca aactgggctt
      361 gttagtgaaa gagacatgaa aagtcaatta cttgacaata tggaccttga aagagaaaga
      421 ggaatcacta taaagcttca aaacataaga ttaatgtata aagcgaagga tggaaatgaa
      481 tattatttaa atcttataga tacacctgga catgtagact ttaactatga ggtatcaaga
      541 agtttagctg catgtgaagg agcactatta gtagttgatg cagctcaagg tgttgaggca
      601 caaactttag ccaatgttta tcttgctata gaccaagatt tagaaatatt accaataata
      661 aataaaatag atttaccaag tgcaagacca gaggaagtaa aaaatgaaat agaagattta
      721 atagggcttg attcaagtga agctcctctt atttcagcta aaactggttt aaacatagaa
      781 gatgttttag aagatattgt aaaaaatgtt cctccaccta aaggagataa tgaagctcca
      841 ttaaaggcat taatattcga ttcttactat gatgcttaca aaggtgttgt agcttatgtt
      901 agagtatttg aaggaactgt taaaaagggt atgacaataa aaatgatgaa taccaataaa
      961 aagtttgagg taactgaagt tggtgttatg gctccaggtc aaactgaact tagtgaatta
     1021 tcagcaggtg atgttggtta tattgctgct agtataaaag atataagaag ttgtcgtgtt
     1081 ggtgatacaa taacagattc aaataatcct acagaagaac ctttaccagg atacaaaaaa
     1141 gctactccaa tggtatattg tggtatatat ccaggagaag gagaaaagta tgagaatgtt
     1201 agagatgctc ttgaaaagct tcaagtaaat gatgctgcac ttgaatatga agctgagact
     1261 tcagcagcac taggatttgg ttttagatgt ggtttcttag gtcttttaca tatggagata
     1321 atgcaagaaa gacttgagag agaatttaac cttgatataa ttactacagc tccatctgtt
     1381 atatatagag ttacaaaaat ggatggagag gttgtaatga tacaaaatcc tgcaaactta
     1441 ccagaaccat ctgagataaa gatgatagaa gagcctatag taaaaggaga tataatagtt
     1501 cctaaagact atgtgggtgt tgttatggag ctttgccagg aaagacgtgg aaatatgtta
     1561 aatatggaat atatagatga gagaagagta atgcttcact atgaccttcc tctcaatgaa
     1621 gttgtttacg atttctttga tgctttaaaa tcgagaacta gaggttatgg ttctcttgat
     1681 tatgaggtta aaggttatgt agcatctaca cttgtaaaac ttgatatatt aatcaataaa
     1741 gagcaagtgg atgcacttag ttttattgtc catgagacac gtgctttccc tagaggaaag
     1801 gcaatgtgtg agaaattaaa aggagaaatt ccaagacatc agtttgctat accaatacaa
     1861 gctgctgttg gtaataaggt tattgcaaga gaaacaataa gtgctctaag aaaagatgtt
     1921 cttgctaaat gttatggtgg agatatatct cgtaagaaga aacttcttga aaaacaaaaa
     1981 gaaggtaaga agcgtatgag acaaattggt tctgttgaag ttcctcaaaa agcatttatg
     2041 tctgtgctta aattagatga gtaaatcctt ataaatttag ataaagatat ttaaaagcta
     2101 gagtgatttt agaattattt ctaaattagt ctagcttttt tatggtatat tttaagtaaa
     2161 ctcatagaga atataagtaa gaatattaaa gaagaataaa aaggtgatat ttatgattaa
     2221 tatagaaatt cctaaaaaag tagactatat aattaaagaa ttagaaaaga atggttatga
     2281 agcttatata gttggaggat gtgttagaga ttgtttatta gaaagagttc caaacgattg
     2341 ggatataaca actagtgcta gaccagaagt ggtagtagaa ttatttgaaa aaacaattcc
     2401 aactggtata caacatggga ctgtaacagt tatgattgaa catgaaccat ttgaagtgac
     2461 aacttataga attgatggaa attatagtga tggtagacat ccagattcaa tagaatttac
     2521 aaataacata gttaaggact tgtcaagaag agattttact atcaattcaa ttgcatacaa
     2581 ttcaaagaca ggattagtcg acccatttaa tggctatgaa gatatacaaa ataagtatat
     2641 acgttgtgtt ggaaatccag tagatagatt tgaagaagat gcacttagaa tgcttagagc
     2701 agttagattt tcagcacaac tcaattttaa aatagctgaa ggtactaagc aaagtattca
     2761 taaaaaagca gatttgataa aaaatgtttc tattgaaaga atccagacag agtttaataa
     2821 aatattagta tctgattcat ctaaattaaa cttattaaaa tcaacaggtt tgttgaaatt
     2881 tataattcct gaaatatgtg aattagaaga tgttactcaa cacaatccat atcacatcta
     2941 tgatgtacaa aaacatactt taatagctac agaagtaatt gaagatgaac tgtatttaaa
     3001 acttacaatg ttgtttcatg atttaggtaa aaaagtaact aaaacaactg ataaaaatgg
     3061 tgttgaccat ttttatactc atagtagaga atctgtaaag attgcaaaga agattttgaa
     3121 aagacttaag tatgataatt atactattaa taaagtttta atattaatac aatatcatga
     3181 ttatagaata gaacctaaaa ggaagataat taaaaagtta ttaaataaac ttgaagatgt
     3241 tgaattattt gaagatttaa taaaagttaa ttgggctgat acacttgcaa aaaatccaaa
     3301 gtatgcaaaa caaaagatat taaatcttat tgagtgtgaa aaagaattta aacatataat
     3361 aaatcaaaag gagtgcttta atcttaaaga tttagctatt aatggaaaag atttgatatc
     3421 tataggtata aaaccaggta aagatatagg atatatttta aataagatgc ttgaaatagt
     3481 cataaataat ccagagttaa atgaaaaaga aatactaaaa gaaaaagcat taaatatata
     3541 tacattttag attcaaatat aaaaaattta attactttaa aaaacataga ataaaatact
     3601 tatttagata aaataaataa tttttagaga atattttaga agttacgatt gtatgatttt
     3661 taaatgcata ggcatttact ctaggactat agactaatat aaaaaaaaac tttgggaata
     3721 ctaaaaccat aaatttataa aattaatatt ttatatactg taaaacagtg aggagggaca
     3781 gtatgcatac tcaactacaa aagactattg gtctttcagc agctctttct actgttgtag
     3841 gtatggttat aggttcggga gtttttttta agccacaagc aatatacact acaactaatg
     3901 gagctccagg tctaggtatt atagcgtggc ttttaggtgg ttttataaca ataactgctg
     3961 ggcttacagc tacagaaatt tcagctgcta taccaaaaac tggtggtatg atgatttata
     4021 tcgaagaaat atatggagaa aagttaggtt ttttaactgg atggatgcaa actgtattat
     4081 ttttcccagg tacatcagcg gcattaggag taatatttgc tcaacaagca tctgaacttt
     4141 taggaatgag cccaaataat atggcaaatg tacttcctat agcaattgga gttatactat
     4201 ttttagcgtt attaaatata ataggttcat ccttaggagg aaaagttcag acagtagcca
     4261 ctattggaaa gatgatacct ttaattttaa taatagtctt tggatttatt aaaggtcaaa
     4321 gtagtgaagt attaaatcca tttgtaggag atggtgtaaa tgcatcaaat gcacttggac
     4381 aagtattaat agctactttg tttgcatatg atggttggat aaatgttgga gctatatcag
     4441 gagaaatgaa gtctccagag aaagatttac caagagcaat agttggtggt ttatcattag
     4501 ttatggcagt ttatattata ataaatatag cttatctttg ggtagtacca gcaagtgaat
     4561 tagcaacagt tacttcccca gctacattag tagcaacaag attgtttgga aatataggtg
     4621 gaaaagtaat aactgtgggt atattaatat cagtatttgg gacattgaat ggatatttat
     4681 taacaggttc aaggatacct tatacattag ctgaaatggg aactttacca gcatctaaaa
     4741 ctcttttaaa agtaaattct ggaggttctc ctgtaaattc aatactactt ataactgtgt
     4801 tagcctgtgt atatgcatta tctggtcaat ttaatctttt gacagactta acaatatttt
     4861 caatatgggt attttatgta ttgactttta taggtgttat gaggcttaga agacaaaagc
     4921 cagatttaca cagaccatat aaagtccctt tatatccaat aataccaatt atagctatat
     4981 taggtggact gtttgtaata ataaatcaaa tattgacttc aacagtcata tctttaggcg
     5041 gaatatttat aacactatta ggtttaccag tttattatta tatgaaaaaa agataactac
     5101 ttaatttaag ttagataaat ttgggtaaag gtgaaaatta acttttatcc aaatttttta
     5161 tactgttata caatataagt attatatatt gcttgatgtg ctattttgtt tgatttataa
     5221 tttagtaatt gatgttttaa gataatttaa gaaatgagga gtgaaagatg ctaggacttt
     5281 atgtacatat tccattttgt gtgaaaaaat gtaagtattg tgattttaat tcttataaaa
     5341 tggacataga ttcaaaaaaa agatatatag aagacttaaa aatagaaatg gaattatata
     5401 gtaataaatt atataaagat aataaatata aaaataaaga atgctgtagt ctaaataaaa
     5461 atgacaaaat aacgagtata tttgtagggg gaggtacacc tagtatacta acctctgatg
     5521 aaataagaga agtatttata agtataaaag aaatgtttga tatagatgag aatgcagaaa
     5581 taactataga gtgtaatcct gggacattaa cattagaaaa attaaaaact atgaaagaga
     5641 ttggcataaa tagacttagt atagggctac aagctattca agaaaagcat ttgaacttta
     5701 taggaagaat acatacttat gaagaatttg agaaaaacta taaagatgca ttaagtgtag
     5761 gttttaaaaa tattaatata gatttaatgt acagtcttcc aaatcaaact ctatgtgatt
     5821 ggaaggaaac tcttgaaaaa gttgtacact taaatccaac acatatatca gcttattccc
     5881 ttatactaga agaaggaact gaactataca atatgtatga aagtaataaa tttgaactta
     5941 tagatgaaaa tgtagatata gaaatgtatg agtatacaat aaactactta aagtcaaaag
     6001 gatataatca atatgaaata tccaactact caaaagaagg ctataattgt gagcataata
     6061 ttttatattg ggaatgtgaa cattatatag ggattggagc aggagcttct ggctatatta
     6121 atgagaacag atacaataat gtagaatctt tagaagatta tcatttaagt ttggtaaaaa
     6181 gagaaaaacc aatacaagaa aatgaaattc tttctgagaa agatatgata gaagaaaaga
     6241 tatttatggg gcttagaatg aataaaggca taaaatttga agattttaaa aaaaaatttg
     6301 gaatagattt tagagagaag tataataagc aaatagaaat gctattagct agaaagctta
     6361 taaatcagag ctttgaaggt atacaattaa ctcaaaaagg aagagaaata tctaatagtg
     6421 tatttataga atttatggaa taatttttaa ggtgttgaca aaataaattt ataatggtat
     6481 ataatatata taatcttttt agcactcgaa cttagtgagt gctaacagct aggaggtata
     6541 ataatatgga attaaatgaa agaaaattaa atatcctcaa agctatagtt aaagattata
     6601 tagaaacagc tgaagctata ggttctagaa caatatctaa aagacatgat ttaggtgtta
     6661 gtgctgctac cataagaaat gaaatggctg accttgaaga attagggtat ttaattcagc
     6721 ctcatacttc tgcgggtaga gttccatctg aaaaaggcta taagttatat gtaaactctc
     6781 ttatgagtaa aagcgaattg gatgataatg ataagattct tattgaacaa tgtatgaatc
     6841 acaatataaa tcatataaaa gaattgattc atgaaacttc taagctatta tcgcaactaa
     6901 caaactatac aactgtagca gttactaaga gtttaataaa tcaaagtgtt ataaagcaca
     6961 tacagttggt tgctatgaat gacaataata ttttacttat agttgtaaca gataaagggg
     7021 acttaaaaaa agctaacctt acaactaatg tatatttaga tcaatctaag ttaaatttga
     7081 tttctgataa tcttacaagg aagctcttag gtaaaagtat aacagatttg gatgataatc
     7141 taattgcttt tattaaatac gaaataagtg agtattctgg gcttatagat gagcttttaa
     7201 atgctttgaa ctcaaatatg aaagaagaag atttttctct atcattaaat ggagctacta
     7261 acatatttag ttatccagaa tttaatgatg tattaaaagc aaagtcattt ttaaatatgt
     7321 tggaaaaaaa ggaaacaata gctgacataa ttaaatcaaa gggaatacaa aaagacaatc
     7381 taaatataat aattggtagt gataatgatt gtgaactggc acaggattgt agtatagtta
     7441 cagcaactta taatgtagat agagacttgg ttgggcgaat aagctttata ggccctacca
     7501 gaatggatta tgctagaatt tactctataa taaattatat gagtttatta attaatagaa
     7561 agtaaagaaa ttttaaattg cgaggtgtaa agtcttggat aaaaaaaatg agcaacagga
     7621 agtaagagaa gaaaatgata cttctattaa ccaagaatcg gaaactcaag tagagttaga
     7681 agaggaagtt gttaatgaag aatgtgaaac atcaagtgaa aaaactgatg aaaaagaagt
     7741 tgatgatgaa aatgtaacag atataaattc taagttagca gaaaaaaaat tgcaggatga
     7801 gttggatgaa ttaaatgata aatatcaaag acttcaagcc gaatatgcaa actacagaag
     7861 aagaactcaa caagagaaag agacaatagg cgtatttgca aatgaaaaaa taataactga
     7921 attgatacca gttatagata gtatggaaag agccttagat gcatgtgaag ataaggaaga
     7981 tactatgtat aagggtataa gccttgtaca taaacaatta atagacactt tagtaaaatt
     8041 tggagtagaa gaaatagaag cagaatctaa ggaatttgac cctaatctac atctagcagt
     8101 tatgcaagaa agtgtagatg gagttgaagc aaatcaaata gtaatggtac ttcaaaaagg
     8161 atataagcta ggaactaaag ttgtaagacc ttcaatggtt aaagtttctt gctaagtata
     8221 aattaaaggt taaattattt aacctaatat aaaatattaa aataagtaaa acttaattat
     8281 ataattactc gaggaggatt ttagtcatgg gaaaaataat aggaatagat ttaggaacaa
     8341 caaattcatg tgttgcagtg ttagaaggtg gagaagcaca aataatagca aatagtgaag
     8401 gtatgagaac tactccatca gtagtagcat ttacaaaaga tggtgaaaga atagttggag
     8461 aacctgcaaa aagacaagca gttacaaatg cagataaaac aataacttct ataaaaactc
     8521 atatgggaac tgattataaa gtaaatatag atggaaaatc atatactcca caagaaatat
     8581 cagcaataat actacaaaaa ttaaaatcag atgcagaaag ttacttagga caaacagtaa
     8641 cagaagcagt tataacagta ccagcttact ttacagatgc tcaaagacaa gcaactaaag
     8701 atgctggtag aatagcaggg cttgatgtta agagaataat aaatgagcca acagcagcag
     8761 ctcttgctta tggtatggat aaattagacc aagaaaagaa aatattagta tttgacttag
     8821 gtggaggaac ttttgacgta tctatacttg aaataggaga tggaactttt gaagttttag
     8881 ctacagctgg taacaataga ttaggtggag atgactttga ccaaatagta atagattatt
     8941 tagcagagga atttaaaaaa gctgaaggtg tagatttaag aaatgataaa atggctcttc
     9001 aaagattaaa agaagcagca gaaaaggcta agaaagaatt atcatcaaca atgagttcaa
     9061 acataaactt acctttcata actgctacag cagaaggacc aaaacactta aatatagatt
     9121 tatcaagagc taaatttgaa gaattaacta gaggtttagt tgaaaaaact atggaaccaa
     9181 ctaagacagc tttacaagat gcaggacttt ctacaggtga tatagatgat gtattattag
     9241 taggtggttc tacaagaata ccagcagtac aagaagctgt taagaagttt ataggaaaag
     9301 aacctcataa aggtataaac ccagatgagt gtgttgcagc aggtgcttct atacaagctg
     9361 gagttttagc aggggatgtt aaagacttat tattactaga tgttactcca ttatctcttg
     9421 gtatagaaac tatgggtaat gtaatgacta agataataga aagaaatact acaataccaa
     9481 ctaagaagtc tcaaatattc tcaactgctg ctgataacca aacagctgtt gatatacatg
     9541 ttcttcaagg tgaaagaagt atggcttatg acaatacaac tttaggaaga ttccaattaa
     9601 cagatatacc accagcacaa agaggaatac ctcaaataga agttactttt gatatagatg
     9661 caaatggtat agttaatgtt tcagctaaag atttaggtac aggaaaagag caaaaaataa
     9721 ctataacatc aaacactaat ttatcagaag cagaaataga gcaaaagata aaagaagcag
     9781 aaatgaatgc agaagctgac aaacaaaaga aagaaaaaat agaagcattc aatcaagctg
     9841 aatctactat atatcaaact gaaaaaactt taaatgaact tggagataaa ataagttcag
     9901 gtgaaaaaga ggatatagaa aaagctatag cagacttaaa agctgtaaaa gataatcaag
     9961 atgctacagc agaagaactt aagaaagcta cagatgaagt aatgactaag ttccaaaaag
    10021 tatctcaaga aatgtaccaa aaagcagctc aagaacaaca agcggcacaa ggtgcagaac
    10081 aagcacaaga caatggacct aaagatgaca atgtagtaga tgctgacttt aaagaagtag
    10141 atgaagataa ataagctata taatatgtaa cactaaatga taattggaac atatatgatt
    10201 tgtgaaaatc atatagatgt taaagatata tggacaatat acatcataaa gaatataata
    10261 tatcatagat tatataactt aaagtagaat ataagaatta atgatagctt gtagtttatt
    10321 attctacaag ctatcatttt gtaaaatgaa ggtggtgtca atttttgagt acaaaaaggg
    10381 attattatga agtcttaggt atcagtaaag gtgcagaagc tcaagagata aaaaaagctt
    10441 atagaaaatt agcaatgaag taccatccag atagaaatcc tggagataaa gaggcggaag
    10501 aaaaatttaa agagataaat gaagcttatg aagttctatc tgatgataca aagagaaaaa
    10561 cttatgacca gtttggacat gatgggctaa atggtcaagg cggttttggt ggtcaaggag
    10621 gatttggcgg tcaaggcttt ggtggttttg aagatatgtt cggagatata tttggtgaca
    10681 tgtttggagg aagcttcgga ggtggaagag caagaagaag agggcctcaa cgtggtgcag
    10741 atataagaca gagtgttact atatcttttg aagaagctgc atttggtaaa aagatgtcta
    10801 taaaagtaaa tagaagtgaa gaatgtgaag aatgtaatgg aactggagca aaaccaggaa
    10861 catcaaagaa aacttgttca acttgtaatg gaactggtca agttagaact gttcaaagaa
    10921 ctccatttgg taatatagca agttcaagac catgtagtgc ttgtaatgga actggagaag
    10981 tgattgaatc tccatgtagt aaatgtcatg gaactggaaa tacaagaaaa gttaagacta
    11041 tagaagtaga tatacctgct ggtatagatg atggtcaaat gataaaactt tctggtcaag
    11101 gtgaagtagg agaaaaaggt gctccaagag gagacttata tattgtagtt aatgttaagt
    11161 cacatccact atttactaga gatggaaatg atatttattt tgaaatgcca attacatttg
    11221 ttcaagcaac tttaggtgat gagatagaag tacctacttt ggatggaaaa gttaaatata
    11281 gtgtgccaga ggggacacaa actggaactg tatttagact taaagagaaa ggaatcccta
    11341 gaattagagg taattcaaga ggagaccaat atgttaaggt tgtagttgaa attcctaaaa
    11401 aattaaacga taagcaaaaa gaactattga gagaatttgc gaaggagtgt gggtcgaacg
    11461 tccacgaaaa gaagaaaacc ttcggacaaa agatagaaga tatgtttaag aaaaagtagt
    11521 taaagtagcc aatatataat catattttta aaatcttagt tatttataag attgtcaaag
    11581 gagatattat gatttttata ttggctattt ttgtataaaa aataaatgtg cataaaataa
    11641 aactgaatat aaataatttt ctgaaaaata cttgtcctaa aatatataat atgctataat
    11701 ataatcaaat aaaagaaagg ggtgattcca gtgggaacgg atgtggcact gccaaatgat
    11761 gaagaaaact ttattgtaat atacaaaata aaattagata cttttagtgc tagattaagt
    11821 actggaaaaa ctcatgagct gggataatta ttgtaattac ctagactcaa acttttagta
    11881 gtaaaatagt aatattatat agacaaggta tctaattttt aaatcttttg tatgaattaa
    11941 aatttttata aagggtatat ctgattagaa tgtaattagg gaaacctagt tgtgaattac
    12001 ttttagtata tacatgtgta aagaaatatg tatatggtat tggttattgt gctagatgtg
    12061 tatgtaaagg aatatgtgtc tagttgggta tatgtaaaga aatatactta taatctatga
    12121 aaaattttta gtataaattg ctaaaacatt gagattgttt tttatagaaa gttagtttat
    12181 accaactaaa aatgtgctta agatttgttt gatgtttaat tataaataag gttgtagata
    12241 aaaaaataag taaagacaaa ggtttaaaat acttgcttta aatatagttt gttttgaaca
    12301 cttgccaaat taaaataatt aataaaatag acatcttctc atagtaaaat atactgtgag
    12361 aggatgtttt tgctttgttc ataaactttt atgaaaaatt t
//
LOCUS       DAELJO010000115        12311 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELJO010000115 DAELJO010000000
VERSION     DAELJO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.115"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..378
                     /locus_tag="KPB88_002542"
     CDS             <1..378
                     /locus_tag="KPB88_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054810.1"
                     /translation="IFTLKNGESKTATKLPAGIDYEVTETEENQDGYVTSKKDDTGTI
                     TKDVTGTAVFTNAKDVTPPEPENPVKPYAPVIADTPNQDVLSPNTGDNTNLNLYISLF
                     IMSGLFIVILEVMYRKRNSKKDN"
     gene            677..1096
                     /locus_tag="KPB88_002543"
     CDS             677..1096
                     /locus_tag="KPB88_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0054811.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVTKGIVRNYQKELKRRKNKEIPFC
                     ELPELAIEKLSVLDDYDSDYTLFNVCGNDIRILDDELAEALKKLSDRNRENLLMYYFL
                     EMSDTEIAKLQNISRSGVFQNRYNSLELMKKLLKGEK"
     gene            1100..1348
                     /locus_tag="KPB88_002544"
     CDS             1100..1348
                     /locus_tag="KPB88_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054812.1"
                     /translation="MKRMFKKPSFYLISSATDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            1730..1933
                     /locus_tag="KPB88_002545"
     CDS             1730..1933
                     /locus_tag="KPB88_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0054813.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            2011..3201
                     /locus_tag="KPB88_002546"
     CDS             2011..3201
                     /locus_tag="KPB88_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0054814.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRELPLTE
                     RAYQAIQRIVKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKSHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(3396..4130)
                     /locus_tag="KPB88_002547"
     CDS             complement(3396..4130)
                     /locus_tag="KPB88_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0054815.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            4255..4971
                     /locus_tag="KPB88_002548"
     CDS             4255..4971
                     /locus_tag="KPB88_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0054816.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            5105..5797
                     /locus_tag="KPB88_002549"
     CDS             5105..5797
                     /locus_tag="KPB88_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054817.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            5787..6968
                     /locus_tag="KPB88_002550"
     CDS             5787..6968
                     /locus_tag="KPB88_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0054818.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            7250..7705
                     /locus_tag="KPB88_002551"
     CDS             7250..7705
                     /locus_tag="KPB88_002551"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG0054819.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            7695..8420
                     /locus_tag="KPB88_002552"
     CDS             7695..8420
                     /locus_tag="KPB88_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG0054820.1"
                     /translation="MGNNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            8392..10773
                     /locus_tag="KPB88_002553"
     CDS             8392..10773
                     /locus_tag="KPB88_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG0054821.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPIVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(10978..12207)
                     /locus_tag="KPB88_002554"
     CDS             complement(10978..12207)
                     /locus_tag="KPB88_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0054822.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         4716 a         1640 c         2215 g         3740 t
ORIGIN      
        1 atattcacat tgaaaaacgg agaaagcaaa accgcaacca aactgccagc aggtatagac
       61 tatgaagtaa ctgaaacaga ggaaaatcag gacggctatg taacctctaa aaaagacgat
      121 acaggaacaa tcacaaaaga tgtaacggga acggctgtat ttacaaacgc aaaagatgta
      181 acaccgccag aacctgaaaa tcctgtaaaa ccttacgctc ctgttattgc tgatacgcca
      241 aatcaagatg tattaagtcc taatactgga gacaatacga acttgaatct ctatatatcg
      301 ttgtttatca tgagtggttt atttattgtg attttagaag tgatgtatag aaagagaaac
      361 agtaaaaaag ataattagaa aatgatgttt ttggatactt aagaaagtgc tttaaagagg
      421 attaacgtta tggaatgttt taagtacaac tctatataat caaatacaat ataataaaac
      481 tttactagaa atcaaatatg atacagcttt aaaggctcgt acaaactata tgttttgtgc
      541 gggctttttt tgtacccaaa aaaatttttt taactttttt cagcttcaac ttagcaaatc
      601 acaccttgct gtacagatat ggcgtggaaa gagggataaa cacttttcaa gtcatagctg
      661 gaaagggggt gagattatga agccgtctga tttccagaaa acaatacaat gtcaatttga
      721 atgtaagcta aagaaagtta caaaaggcat tgtaagaaat tatcaaaaag aattaaaacg
      781 gagaaaaaat aaggaaattc ctttttgtga gcttccagaa ttagcgattg agaaattatc
      841 cgttttagac gattacgata gcgactatac attattcaat gtatgtggca atgatattcg
      901 tattcttgat gatgaattgg cagaagcctt gaaaaagctg tctgaccgta accgagaaaa
      961 tttactgatg tactatttct tagaaatgag tgataccgag attgctaagt tacagaacat
     1021 ttccagaagc ggagtttttc aaaatcggta caactcgttg gaacttatga aaaaattact
     1081 taaaggagaa aagtaaaaga tgaaacgcat gtttaagaag ccgtcttttt atctgatttc
     1141 atcagctaca gatggggacg aaaaagccat tgaaaaggta cttgcatttt atgaccctta
     1201 catatcaaag tgctgtttgc gtccactcta tgatgaatac ggtaatgttt atatcgttgt
     1261 agatatggag ctaaaaggac ttatccgaga agcacttatc aaaatgattt taggatttga
     1321 tattgcctta gaaattgaag aagaataaca agcagtaacg cagagcatga tttgttcaat
     1381 cccctctttc ctgctctgct cctgctttta catgaaagca taccgctttc gccacagctc
     1441 tttgaaaact gaataaagca gacagatacg tttgcaagga tagagagcca cgagaactgt
     1501 acgccatgac ctttccaaaa gaaagcgagc gactcacaca gtgatccgag agcgactggt
     1561 ggaaaagtcg ttttgccatg acctaccctt acagcataat gatacgtccg catggtgcgg
     1621 ttctgcccac aagaatggga atagttgaaa tactaacgga gctttccaaa gccgtctgcc
     1681 tgcttatata acttatagac tgacacacag taaagggggt gcataaacta tgaacaatac
     1741 tgatgtgcct atctgggaaa aatatacgct gacgattgaa gaagcgtcca aatacttccg
     1801 cattggagaa aagaaattgc gtaaattagc cgaagaaaat attgacgctg gctgggttat
     1861 cgtgaacggc aaccgtattc agataaagcg aaaacaattt gaaaaaatca tagatacatt
     1921 agacgaaatc taatgccata gagacgtaga tatggtataa taaatatagc gtatcacggc
     1981 tcattccatg acggaaagga gctttacact atgtctaatg tgaaacgaaa agacagtaaa
     2041 aatcgcaatt tgcgtaatgg agagagccag cgaaaagacg gaagatacgt ttataaatat
     2101 accgatatat acggaaagcc acagtttatt tattcttgga aacttgtacc gacagacaaa
     2161 acacctgctg gcaaacgtga tgatatatcg ttgagggaaa aagaagcaca gataaaaaaa
     2221 gaccttaacg acggtatcga cacagtcggc ggtaaaatga cagtctgcca gctctacgac
     2281 aagaagaaca gccaaagaaa gaacatcaag agggctacgg aaaaaggacg acagtatctt
     2341 atgaacgctc tgaaaaatga cccattgggt atgagggcga ttgataccgt caaacagtcg
     2401 gacgctaaag agtgggctat cagaatgagt gaaaaaggat atgcctacaa aacgattgac
     2461 aactataagc gttccttgaa agcgtcattt tatatggcga tacaagacga ctgtatcaga
     2521 aagaacccgt ttgaatttaa gctaagtgat gttctggaag atgatacaga gccgaaagtt
     2581 atccttacac cagagcagga agaacgcctt cttgccttta tggaaacaga taagatttac
     2641 agcaagtatt atgatgaggt tgtgcttctg ttggaaacgg gacttcgtat ttctgaattt
     2701 tgcggactga caacgcatat tgatatgcag aacagaatac tcaatataga ccaccagtta
     2761 ttgaaagata gcgaaatcgg ctactatatt gaaacaccaa aaaccaaaaa cggaaaacgg
     2821 gaacttccat tgacagaacg ggcttatcaa gcaatccaaa gaatagtaaa gaacagagga
     2881 aaagcacaac cgctgattgt aggtggttac agcaatttcc tattcttaaa ccgtgagggc
     2941 ttacctaaag ttgcaggaaa ctatgagggc atggtgcgag ggctgattaa gaaatataac
     3001 aagtcccata aggacaagtt accgaacatc acaccacatt cattccgaca cacttattgt
     3061 acgaatatgg caaacaaggg aatgaacccg aacaccctgc aatatctcat gggacacgct
     3121 aacataacta tgacgctggg ctactacgca cacggtacat tccaatccgc aaaagcggaa
     3181 ctggaaagac tggcttgtta atatcggaac gtatatttac tactcattta ctacttttgg
     3241 ttgcattttc atgctggtaa atgccagctt atgcaaggta tcttccaaaa agaaaatacc
     3301 gataaagccc taaaatacgg gatatatcgg catttaccag cttatgaaaa gataatcaga
     3361 aatttagtaa gttttttatt taaaaatttt aattattatt taaaattttt tatcttctct
     3421 ttatcccata taaacaattt ctcagaatta tttaaagcac caaatacatt tcttttaaaa
     3481 tcaggcatct ctttatttaa attagcaata agatttttta tgtactctct cctagtatga
     3541 ccatttgcag ggcagggatt tgtgataata ggatagttag aatcttttga aattttctta
     3601 gtcatatact cttctatgta aaccataggt cttataactg ttaaatcctg tctgtctagg
     3661 tatgtctttg gggaaaaaca atttactcta ccctcataaa acatagacat taaaaatgtc
     3721 tcaattgcat catctttatg atgaccaaga gctactttat tacatccaag ttttttagca
     3781 ttatcgttca aagcacctct tcttaaatta gcacacaatg cacaaggatt cttttcttgt
     3841 cttatatcaa atacaatttc ttttatgtca gtttgaaatt cataaaaagg aacatttatt
     3901 tctttacaca atttatctag tggagaatta tcaacacctc cagtatttaa tgttatggct
     3961 atcaattcaa agttttgtgg agaaaatctt tgataacttt taagtatatg aagtagagtt
     4021 aaactatctt ttccaccaga aagtccaacc gcaattttat cattttcttg tatcatatca
     4081 aaatcttgta tagccttacg agctttactt agaagttttt gtatagacat tttatcacca
     4141 tcctttaaaa atattaaatt taagatttat ttaagattta cttataaaat acttaagtct
     4201 tatactctaa aattaacatt atagcaaaca taacaaaaag gagaagattt aattatgaaa
     4261 aaatttaaat taataggagc cattatttta gtagtattta taagtgtatt tatatctaaa
     4321 gatagattac ttaagaaaga taaagatcca atagtagctg ctcaacatga ttcagaacaa
     4381 gatgttgagg caaattctca aaataaaaat cactcaaata gtatatcaaa tatattattg
     4441 gtaaacaaga caaatggaat tagcaaaaac tatactcctg aaaatattac taaggtaaac
     4501 ataccttttg ttgaagaagc aacagaggaa gaaaaacaaa tggcaggaga acctgcaaag
     4561 gcagttgaag atttagttaa gcaagctaat tcagaaggaa tacagttttt agggtcttca
     4621 gcatacagat cttatgatac tcaactggat acttatacaa gaagggtaaa atctcagggg
     4681 cgagaaaagg cagatgcata tgtagcaaag cctggttaca gtgagcacca aactggactt
     4741 tgtattgatt taacaaatcc agaaagatgg tttgttggaa gtacaaaaga agctaaatgg
     4801 ttggcagaaa atgctcacaa gtttggattt ataataagat atcctgaagg taaagaagat
     4861 ataacaggta cagcatatga accatggcat attagatatg ttggtaagga tgctgctgag
     4921 gaaatttatt caaaaggatt aactttagaa gaatatttac aaaataaata atataaatgt
     4981 gtaaataatt tgtattatat tggaaattta gaaattaata aatttataga ttacactaac
     5041 taaaatatag tataatttta tatgaataaa gctatataca aagattaagt taggatgtgt
     5101 aaaaatgaat aatacagttc ttgttgtgga tgatgaaaag gaaataagag accttataga
     5161 gatatatctt ttaaatagtg gatataatgt aattaaagct gaagatggaa aagaggctct
     5221 agatatttta aaaaaggaaa atattcattt aatgatattg gatataatga tgcctaaaat
     5281 ggatgggata gaagtttgta gaaaagttag agaaagtttg aatataccaa tacttatgtt
     5341 aagtgcaaaa tcagaagaca tggataaaat acaagggata atgactggtg cagatgacta
     5401 tttgacaaaa ccatttaacc cacttgaact tactgtgaga gtgaagtcgc tgattagaag
     5461 agcatattat tttagtggtg ctaataatga agataaagat gtcataaagg ttggagtagt
     5521 aattatagat aaggaaaaac atagtgtaac agtggctgga aatgaaataa ttttgacatc
     5581 aagagaattt gatatattat atttactagc aaataataaa ggcagagtat atagtacaga
     5641 agaaatattt gaaaaggtat ggaaagagaa atattaccaa tctaataata ctgtaatggt
     5701 tcatatgagc agaataaggg ataaaattga aaaatataca gatggagaaa aaataattca
     5761 tactgtatgg ggagtaggat ataaaattga aaaataaaag tttattaaat catatgtttc
     5821 gacccatatt agatcttata ataagctgtg ctttaacttc aatgcttatt ttaacaagta
     5881 tgtatataat aaaatcatta tatgtcaata gtgatatgtc tgtagttaat caacttgtat
     5941 atcaagcaag agatttcaga agtcttatag ggtatgattt attttatcca tttttattta
     6001 caatcacatc attagcaatt tattccatta tatcgtacaa gagaaataag caaattgtag
     6061 attttataag aaagactaat agaaaaaatc aaaaattaga aatcattaaa agtgctgtaa
     6121 atttaatgga tgaaggtaac ttagaaaaaa gtatagatat tgagtatgat ttagatatat
     6181 taaaggataa agaaaaggat gatattgatg aactagcaca taaaatcaat aatatagtcc
     6241 atcaacttag aaatataact attgaagaga gacaagctca gcagacaaaa acagatttaa
     6301 taacaaatgt gtcacatgac ttaagaacac ctttgacatc cataatgggt tatttaggag
     6361 tgatagaaga aggtaagtat aaagatgaag ttcagatgat gtattatgtt gatattgcat
     6421 atgaaaaatc gaaaaattta aatgttctta taaatgattt gtttgaattg actaaaatgc
     6481 aaaataatac aataaaatta aacaaaatag aaataaattt agtagagtta ttaagtcaag
     6541 tagtatctca atttgaaatt tattttaaac aagaatttat ggttagtaga attaatttta
     6601 atgaagaaaa attaattgta aaagctgatc caaataaatt ggtaagagca tttgaaaatc
     6661 ttataactaa tgctgtgaaa tatggaaaag atgggcatta tgtagatata gtcacagaga
     6721 aaaaggaaga tatggcggtt gtaaaagtga taaattatgg ggaaccaatt ccagtgttag
     6781 atttaccaaa tatatttgat agattttata gagttgaaaa atcgagaaat agaaatgttg
     6841 gaggttctgg attgggtctt gcgattacaa aaaatattgt aaatttacat gatggagaaa
     6901 ttactgtttc aagcgataaa tatcaaactg tgtttgaagt acaactgcca ataatacaat
     6961 cagcttagtt ataaaataaa agtacgatta tgtgatataa atattataac aataaagtgt
     7021 ttacaaaaat aaaaaagtaa tatataattt aagtcagttt aataagatac ttatcaagag
     7081 ttaggggaga gagttagctc agtgatccta taccaacctt ttatcaggtg gtcctgctaa
     7141 caacgatgag atgacttgaa ataatatact cttcttatca ataatagata ggaagagttt
     7201 tttttagaat taatgtaaca aattttaaag tataatggag gattaaataa tggaaaaagt
     7261 agaaagtttt aagctagacc atacaaaagt aaaagctcct tttgtaagaa agtgtagtgt
     7321 acttgatgga gtaaagggag ataaagtaac taaatttgac ttgagatttt tacagccaaa
     7381 tgtagaaagt tttggaactg ctgctatgca cggtttagag catcttttag ctacttattt
     7441 aagagataca ttagatggtg taatagactt atctccaatg ggatgtagaa caggatttta
     7501 tttaatacta tggggagatg tagatgccaa aacagttaag attggtttag aagaagcatt
     7561 aaaaaaagtt cttgaatcag ataaaatgcc agcagctaca gctatagaat gtggaaacta
     7621 cagagactta tctttatttg gggcaaagga atatgcaaaa gatgttttag ataaaggatt
     7681 ttctttaaat atatatgggg aataatttaa ttggaagaga agatataagc ataaatcaaa
     7741 gtgaagtttt aagatattta caatacagag gtcaagagat agataaagac cttaaatata
     7801 ctattgatga atgtgtagct ataactaaaa ataaaataaa tcctagatat ttatctagga
     7861 tttatcctat taaaataaat aaagataatg ttgtagaatt agagggaaca aatttaacat
     7921 tagagagtaa agatgtatat gaacttttaa aagaatgtaa tgagtgtgta attatggcag
     7981 ctactatagg gatagatata gaaaaagaaa taagacgaca ctcttattca aatttaacaa
     8041 aaggactaat aattgattca tgtggaacaa cagcaataga agaagtgtgt gacattgttc
     8101 aaaataattt agaaaaggtt tttttaaagc aagataaatt tataactatg cgatatagcc
     8161 caggatatgg agatttacca atagaaaaaa atatagatat attaaacgtt ttgaatgctc
     8221 aaaaacaaat aggtctaaca attacttcta atggaattat gataccgaga aagtcagtag
     8281 tggctattat aggtatttct aatacaagaa taaataaatc taagaaagaa aagtcgtgtg
     8341 aaaattgtaa aaactataga aactgtacat atagaaaagg agcgaactct tgtggaaata
     8401 agacaatatc tgaaaaataa tatattaata tttgatggtg caatgggaac aatgcttcaa
     8461 caaaaagggt taaagttagg tgaaaatcca gaagtatttg gtcttcaaaa tccagataaa
     8521 cttatagaaa tacatacagc atacttagag gcaggttcga atgtaattct tacaaatact
     8581 tttggatgta atgaactaaa attagatagt aagtatacgg tagaagaagt gattgacaat
     8641 gctgttttgg tggctagaaa agctatagaa aatgtagata atactaaacc aagatatgta
     8701 gctttagata ttggtcctat aggagaaatg ctagagccta tgggtacttt aagttttgat
     8761 aaagcatatg aaatatttaa aagacaagta ctacaaggcg ttaaatctgg agttgatgta
     8821 atagtaatag agactatgat ggatttatat gaagctaaag tagctgtatt agcagcaaaa
     8881 gaaaactctg atttacctat tttctgtact atgacatttg atgaaggtgg aagaagtttt
     8941 actggatgta tgccagaatg tatggtggct actatagaag gtctaggtgt agatgcaata
     9001 ggagttaatt gttcattagg acctaaacaa cttttaccaa tagttgaaaa aatagcatca
     9061 agagctacag tccctgttat ggttcaagca aatgcaggtc ttccaaatat agttgatgga
     9121 gaggctatat atgatgttga tgcaaaagaa ttttttgaag gggttaaaaa gttcgttgaa
     9181 gttggtgcta ctataatagg tggatgttgt ggtacgaatc caagttttat aaaagaaata
     9241 tctgagaata taaattctgt tacaaaggga tgtatcgaga aaattgataa atgtgtagtc
     9301 tgttcaccat caaaatttgt ggaagtacaa tcgcctatag ttgttggaga aagattgaat
     9361 cccacaggta gaaaatctct acaagaagca ttaaaaaatg aaaatgttga ttatgctata
     9421 aatttaggtt tagaacaagt aaatgctgga gcacaaatac ttggagtaaa tgttggtctt
     9481 ccagagattg atgaaaaaaa actaatgcca aaattaataa gagagattca agcagtagta
     9541 gatacacctt tacaagttga ttcttccaat gtagaggcct tagagcaagg acttagatat
     9601 tataatggta gaacaatagt aaactctgta aatggcaaag aggagtcttt agaaagtata
     9661 ttaccaatag ttaaaaagta tggttcatgt gtggttggac ttacacttga tgaaaagggg
     9721 atacctaaaa aggctgaaga aagatttgaa atagcaaaaa ggatagttaa tagagcagtt
     9781 tcttatggga taaaaccaaa agatatattt atagactgtt tatcattaac tgtttcagca
     9841 caacaagaag aagcaataga aactataaaa gctataacaa tggtaaagac actaggagta
     9901 aaaactatat taggtgtttc aaatatttca tttggtcttc caaataggaa agcacttaat
     9961 gcttcttttc taacattagc tttaggggca gggcttgatt tagcaattat aaatccaaat
    10021 gaatatagta tgatggaagc tatcaattct tttaaaatat taaataacac agataaagga
    10081 tgtataaact atataaatca atatagcaat ataaataatt caaaaaaatc ggattcatct
    10141 acaaagattg ataaagattt gcccttggat attttagttg aaagaggatt aaaagatgaa
    10201 gcaaaaaatg taacactgaa tcttttaaaa gagaaggatg aaaattatat attagatgag
    10261 attttaattc ctgctcttga taaagtagga aaaagatatg atagtggaga tatattttta
    10321 ccacaattga ttcaatcagc agaaacggta aaagtttcat taaatacaat aaaggaaact
    10381 ttattaagta aaagtagcaa taatgtatcc aaaggaaaaa taatagttgc tacagtaaaa
    10441 ggtgatatcc atgatattgg gaaaaatata gtaaaaataa tgctagaaaa ttatggatat
    10501 gaagttatag atttaggtaa agatgtccct attgaagaag tagtggaagt tgctaaaaag
    10561 agaaatataa aattagtagg attaagtgca cttatgacta cgactgttca aagtatgaaa
    10621 gatacaattc aggcactgag agataatgag ataaatgcaa aggtatttgt aggaggtgca
    10681 gttttgacag aagaatacgc tgaagaaatg ggtgctgatt attattcaaa ggatgcaaag
    10741 tctgctgttg aaatagcaaa gttaaatttt tagcatataa attaaaataa aggttttact
    10801 aaataggata ttttatatat aatataaata taagttttag atacttatgt ttaaaacaat
    10861 aggaattaat ctataataat gagattaatt aatataaaaa atatttatca tcaacattaa
    10921 aaagggctgg gaagtctggt ataacttcca tcagcccttt ttagttaaaa tcatatgtta
    10981 aaaagcccaa ttaccatttt taaaaatctg tatttgcttt ccatcataag tctcaccaat
    11041 aatatccata tcagcagaac caatcataaa atctacatgt gttaaactgt catttacacc
    11101 atgttcatcc aattcttcat cttttaaatt atttccacct tccagacaag tcttataagc
    11161 agaaccaatt gcaaagtggc aagaagcatt ttcatcatat agagtattat aaaaaattat
    11221 atccgtattg gatattggtg agttgtatgg aacaagagcg acttctccta aataatgaga
    11281 gccttcatcc gtttcaataa gtttttctag tgtatctagt cccttttcag cagagaaatc
    11341 tattatttta ccatctttaa atgttaagaa gaaattgtca attatgtttc caccataaac
    11401 aagaggtttt gtactataaa cagttccatt tactttaaac ttatgtggca ttccaaaaat
    11461 ctcttcagtt ggaatattcg gattaaaact tattccatta gggtctttgg atgctccact
    11521 tagccaaaca tgaccctttg gcaagtctag agtcaaatca gtttttgagg atttatattt
    11581 taaagtttta aagttatttt tatttagaaa gtccatttta gatttaagat tttcattatg
    11641 ttcttcccat gcttttatag ggtcctgacc atctactctt gaacatttaa atatagcatc
    11701 ccacagtttt tctattgctt tatcttctga taaatttgga aacacttttt ttgcccaatc
    11761 ttttgttggc acagagacta tactccattt acatttatca gttagagtgt aagaacgcca
    11821 ttcttttaaa gcttttccag atgccttctg aaaatttgca actctttcag ggtcaacatc
    11881 ttttaataaa tctggatttt gggcatagac tgataaaaat gccccacctt cttttgctat
    11941 atctacatat tgttctgctg tccatttggg gaactcatga aatgcttcat ctggagcata
    12001 taagtattta ataagagtac tttcttcatc agaccactct atataaacat ttttagcacc
    12061 acatttatat gcttctttaa gtgcatttct tgcaaaatca gcacattcta ttggtgactt
    12121 tataagtaga atttgacctg gctgtatgtt aactccaact tttattgcta attcagcgta
    12181 tttttctaaa tttctattta aattcatatt tatcctccta tatttttaat attgaataac
    12241 ttataaagta ttataacatt acttatgttt ttaatattga acaacttata aaatattata
    12301 actttactta g
//
LOCUS       DAELJO010000116        12251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELJO010000116 DAELJO010000000
VERSION     DAELJO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.116"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            308..1699
                     /locus_tag="KPB88_002555"
     CDS             308..1699
                     /locus_tag="KPB88_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0054823.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1952..3745
                     /locus_tag="KPB88_002556"
     CDS             1952..3745
                     /locus_tag="KPB88_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0054824.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3826..5208)
                     /locus_tag="KPB88_002557"
     CDS             complement(3826..5208)
                     /locus_tag="KPB88_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0054825.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5466..5813
                     /locus_tag="KPB88_002558"
     CDS             5466..5813
                     /locus_tag="KPB88_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0054826.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5831..6865
                     /locus_tag="KPB88_002559"
     CDS             5831..6865
                     /locus_tag="KPB88_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054827.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6914..7963)
                     /locus_tag="KPB88_002560"
     CDS             complement(6914..7963)
                     /locus_tag="KPB88_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054828.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8238..9773
                     /locus_tag="KPB88_002561"
     CDS             8238..9773
                     /locus_tag="KPB88_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054829.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9958..10644)
                     /locus_tag="KPB88_002562"
     CDS             complement(9958..10644)
                     /locus_tag="KPB88_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG0054830.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10769..12151
                     /locus_tag="KPB88_002563"
     CDS             10769..12151
                     /locus_tag="KPB88_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0054831.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4683 a         1484 c         2030 g         4054 t
ORIGIN      
        1 aaagatatta aaaagaaata tttttaattt gaattttttc aataaaaatt tacaaaaatg
       61 aaaaaataaa caaaaaagaa ttgaagattt aagttgttat ttaaatatat atatattaag
      121 attttctgct gaagatacta aaaaaataat atttttcata attttaaaaa taataataaa
      181 aaaatgaaaa tacaggcgat aatctattga gtaaacatag aaaagatgat attataagta
      241 tgtgctaaaa tggttaaagc aatattaata ttaaatttaa ctataaaaca aaagggggcg
      301 tttttttatg tcaagtacag aaagcgtatc taaaaaaagt ggtcatccga agagtttttg
      361 gcttatttgc atgacaattt tgtgggaaag atttagttat tatggtttaa cagctttatt
      421 ggtattattt tttacagcaa gtgtagctca aggtggttta ggtctttcta ctcaagaagc
      481 aacatccatg tttggcttat ttacaggtct tatatatctt acaccattag tcggtggctg
      541 gttagcagat aaagttttag gtcaacaaaa atgtatttct ataggtgcac ttttaatagc
      601 aataggagat ttcgtactat ttgcttcatc tacatctatt gcaatggttt gggtaggtct
      661 atcaataatt attttaggaa atggtttttt taaatcaagt tgctcaaata tagttgggga
      721 tatatatcct aaaactgatg tgtcaagaaa agatgcagca tacagtttat tctatatggc
      781 agtaaatgta ggagcattta tagctccaat aatatgtgga ttgctttctg ataatgtatt
      841 tgcagtaaga ggagcaagtg gagatatagt atcttatgga tataagatgg catttttaat
      901 ttgtggtata ggcatgatgt taggtatgat tatatttaca cttcttgcac caaaattatt
      961 aaatgaagtc ggaaaatatc ctgtgactaa aggtaataaa ggtgaaaaaa ttgagtatgg
     1021 accacttact aagttggaga aaaataggat aatagcaatg gttattttat ttttatttgt
     1081 ggtcttattc tggacagcat actatcaaac acaaagttct tttatgattt atgtaaggga
     1141 taatgtaaat agaactctat ttggatttga aatgccagta gcatggctta catcactaaa
     1201 tgctatatta tgtgtggctt tatgcccagt attagctacc ctatgggtta aattatctca
     1261 taccaaaaga ggtgatttct ctattccagt taaaatggga ttaggaatga taataatggg
     1321 tgttgggttt atagttatgg tatttgctac attagctaat ggtccagatg gaatggtaaa
     1381 ggctagtata ttgtttatag tattgactta tatatttaca acagtaggtg aattattctt
     1441 atctcctata ggacttgcaa tgttcaataa attagctcca gctaagtatg ctacgttgtc
     1501 tatgggagcc tggtatctaa caagtttttt tgcaagttta ttatcaggaa agattgctgg
     1561 ttttacagcc acacttggtt ttttacagat atttggagga atagcaacag ttttaataat
     1621 atttggttta atattattgg gaataagaaa tgtattaaca agattaatgg ctatggattt
     1681 actaacaaaa gaaaaataat tcatgacgaa ttattgtaaa taaatgaatt actttaaata
     1741 aaggaagtta catttaaata taaaattaaa tgtaatttcc tctttgatta tgaaaaaaag
     1801 ctttacacaa atagatatta taattaaaat tataattgtg ataattagag tgtataagat
     1861 ttatataaat tatacaaaaa atatttatgt aataaacttt acatacttat atatttagtt
     1921 attcaaacta ttatttcgag gaggtcttgt tgtgaatata aaagatagat taagtggatt
     1981 aagaaaattt atggaagaaa aaaatataga tgcctatatg attccatctt ctgataatca
     2041 ccaaagtgaa tatgtaggag attacttcaa atcaagagaa tttatatcag ggtttaatgg
     2101 ttcagcaggg acggttattg tgactaaaga cgaagctggg ttatggacag atggaagata
     2161 ttttatacag gcagaatctc aattagaagg aagtacaata aaattattca aaatgggaca
     2221 agaaggttgc ccaactacag atgaatattt atataaaaat attcctgaag gaggaacttt
     2281 aggatttgat ggtagagtta tatctgctag agaaggagct acattagctg aaaaattatc
     2341 taaaaaaggt ataaagatag aatatcagta tgatttaata gatggtatat ggccagatag
     2401 acctgcttta tcagatagta aagcattttt attagatgtt aaatattgtg gagaaagttt
     2461 tagctcaaaa ttagctagat taagagaaaa aatgagtgaa aaaggtactt ctactcatgt
     2521 aataactact cttgatgata tagcatggtt attcaatata agaggtggag atgttaaata
     2581 taatccagtt gtactttctt atgcagtaat aactttaaaa gaagtttatc tatttgttga
     2641 tgaaagtaag ttaaatgaag aaatactaaa tgaactagct aaggaaaatg ttcaaataaa
     2701 gccatataat gatgtgtatg aatttgtaaa aaatatagac aaaactgaaa aagttttatt
     2761 agatggaact aagcttagtt atacaattta caataatata ccttgtgaag ttgagaaagt
     2821 tgatgagttc aatccagtaa tgttttttaa agctcaaaaa aatgaggtag aattagaaaa
     2881 tataagaaat tcacatgtaa aagatggtgt agctttcact aaatttatgt actggttaaa
     2941 gaaaaatgta ggtaagatgg aaatcactga aataagtgct actcaaaaat tagaggactt
     3001 aagaagagag caagaaggtt tctttgagcc aagtttcaat acaattgctg catataaaga
     3061 gcatgctgca atgatgcact atagtgcaac tccagagtca aattataaat tagaggcaga
     3121 aggattattt ttagtagatt caggtggaca atattatgat ggaactactg atataactag
     3181 gactacagtt ttaggaccta taagtgatga attaaaacta cactttactt ctgtagcaag
     3241 aggaatgata aatctttcta aggctaagtt cttgcatggt tgtagaggat ataacttaga
     3301 catactatct agaagttgca tgtggaatat gggaattgat tatcaatgtg gaactggaca
     3361 tggtatagga tttgtgctaa atgttcacga agctccaaat ggatttagat ggagagtagt
     3421 acctgaaaga tttgatagtg cagttcttga agaaggaatg gtaactacta atgaaccagg
     3481 tatatatata gaaggttctc atggtataag aacagagaat gaaattgtag ttagaaaagc
     3541 tgaaaagaat ttctatggtc aatttatgga atttgaagtt gttacgttag ctcctattga
     3601 cttagatggt atagtaccag aattaatgaa taaggatgaa aaagattatt taaactggta
     3661 tcataagtta gtttatgata aaatatcacc tttcttaact gatgaagaaa gagaatggtt
     3721 aaaagtttac actagagcta tatagtttgt actagataca ataaaaatcg aggtattata
     3781 atgcctcgat tttttattgt atattttatt aactttaaaa aatttttaat ttgataattc
     3841 tactaatttt ttattcatta taagtagtat aattccaaat atcaataaaa atgatggtat
     3901 cactatgaag acttgtaaca taccaagttg gtctataata gcttgcgtat agccagataa
     3961 tttatttgct gcaaatttgc ctaaaaacca aacacccatt aggaatgaaa tatacttttc
     4021 aggcgaaagt ttatttacca taccataccc gactggagaa aagcaaattt ctcctatact
     4081 ttggaatgtt aaaaatccaa ctacccaaaa tacacttgct ttgtgaattg aattagggtc
     4141 tgttcctgtt tgtataactg ctactatcat aaacaaaaat gctatggcta atagtataaa
     4201 acctaagctc attttttttg gcacacttaa atcgcctttt ttagtttgag ataattttac
     4261 ccatactaaa gcagagatag gtccaagaat aacacaaagg agaccattgt atgaatctat
     4321 ccatgaagta ggaatttgaa aattgccaac agtaagattt atgtgttcct ttgtatagag
     4381 tgcaatagac atagaggctt ggttatatgc tatccaaaaa aatactgtga aaaaagctaa
     4441 caccaatatt actattattc tttttttctc aatcaaagtt aaaggtcttg aagcgagcat
     4501 tttcttatat tctagtgatt ttcttataga ctctctcccg ttaatagggt cattattcaa
     4561 aggtttataa aactttctgt ttccaaatat aaagaagaat aaacctatta gaaccgtaat
     4621 tgatgaaaat aaaaatacat atctatatcc atacatactt atagataatc cgcttttcat
     4681 agcaaaccat tcattagcaa ctaaaccgca tattaaaggg ccaaataaag tacctagatt
     4741 agtagccata tacgtaatag agaaagctcc atcttttctt gggtcatcat tggtatataa
     4801 gtttccaacc atagtatgta agtttccttt ataaaaacct attcctaatc cagataaaaa
     4861 tatcatgatg tttatatcca tcttactatt tgctatgtat cctagaaaat aaccaattga
     4921 aaccattagc attccaagta taatacagtt tttgggacct aaaaatctat cactgaccca
     4981 tccaccaata actggagaga aataagtcca tgctaaaaaa gatgcaagca ttgatgcgga
     5041 ttctgctggt gttaacccta aaccaccttt ggagacttgt tcagctaaaa acaataataa
     5101 taccaatttc ataccatagt atgcaagtcc tacaaaggta aatattgaac agcagaaata
     5161 taaatctcta ggttgtttaa gtttttcttg cgtttttaaa actgacatat gtcgagaacc
     5221 tccagataaa tagtttaaca aaattttacc atactatatg agtatatgtt tttttcatat
     5281 agacgtcaac ttgtaagata atttaattaa gtcgaaatat atgtaaaaat agtatcattt
     5341 ttgtgtattc agtaatattt ttttatgata atatatgtta ttattatatt aaaatacaaa
     5401 catatttatg gaaaggatat gccaatttag tagttagatt gattgtttgg caggtgggat
     5461 gagctatgat aagaaagaaa tctgaattac aagtagaagt aaaagaaaat ttaaatggtg
     5521 gtataggaaa ggttaagctt gaaaacataa tacaaaatga agagcttaaa ggaaaaggaa
     5581 gactatttaa aagagtagct ttaccagtag gaagttcaat tggtgtgcat gaccatacaa
     5641 cagattttga agtatattat atactaaagg gaaaaggtaa agtatttgat aatggggaat
     5701 ttgtagaagt aaatgagggt gatgtagtgt atacagctga tggcgaaaaa cattctatag
     5761 aaaatatagg agaagaagat cttgagtttg tagcactagt tttatatatg taaaatatgg
     5821 aggaggagtc atgacaggca agatagaaga aattaaggac aagataaata gattttgcaa
     5881 ctccaattta aatcaagaat acaaagatat aagctttaaa attttgcaaa accttttaga
     5941 taataataaa gagataattc attcttctag agctgatata tggtcttcag ctatactaaa
     6001 tatagtttta gaacaaaatc ttttatacaa taagaagcat ccactacata taacaaaaaa
     6061 agagttttca aaacaaatag gtgtttcttt aaatactata aataataaat cgtctttaat
     6121 aaaagatgtt tgtagtattg actttttaaa tcaagatact atagctattt ctaatgtaga
     6181 attttgggta aaagagacta attccaaatc aaagtctgta gatttagatt tagaaaagaa
     6241 gaaaatacta tataaaagat atattaagct atcacaagat gcgaatagtg atattgaatc
     6301 aattagatat atggaggaag ctgtaaatat aggtaaaagt atgattacta aagaagataa
     6361 agaacttgga ttttggaaag gtttatctac cagagcctat atgatagcac ttgaatccct
     6421 tgctagaaag ttagaaagta ttaataatct aaaggagtcc aaaaaagtat tagaatattt
     6481 aatagaaatt aatcctagtg atgagcaggg aataagatac aaattattaa atgtattaat
     6541 tagattaaat gatagaactt caataaataa attatttgaa ctttataaag aagaaaaatc
     6601 tgctacatgg atgtacagta aagctcttta ttactttaaa aataaaagta tgtttttggc
     6661 aaatgattct attaaggttg ctagaagtaa aaataagtac gttggattat atttgttaga
     6721 ttggcgaaat gcttttggta gagaatttgt tacagaggaa gaaaaggcag aagctgtcta
     6781 ttactatgat gaaaacatag tgatttggaa tgaagttaaa ggttctatgg attggctatt
     6841 aaaaaaaatg ttggaatttt cataaaaaaa actactcctt acttttatat aagtcaaagg
     6901 agtagttttt aatttataca tctagtttta aatcttcagg ttttattaga cctttttttc
     6961 tcataaattc tgaaatcaac aaagttacaa tagcaggaag tataaaatta agtaaaagta
     7021 taccaaccca catagatata cctccttttc cagcactttg catagcagtt actgttccaa
     7081 attgtccaac caatccacaa gtacccatac cagaaccaat aggaatattc tccattttga
     7141 atattgttgt agataatggt ccaagtaata ttgaagctac agtaggtggt atccaaattt
     7201 tccaattttt tattatatta ggtacttgca acatagatgt cccaagacct tgtgccacaa
     7261 ggccaccaac tccattttct ctaaagctca taacagcaaa gcctaccatt tggcaacaac
     7321 aacctacagt tgctgcacca ccagcaagac cacctaaact taacatcata caaatagctg
     7381 cactacttat aggtaatgta agagctattc caacaagtgc agagactaaa gctcccatta
     7441 ttataggttg catagttgta gcttgcatta ttatatttcc aaatgaagtc ataaaagcag
     7501 aaacagctgg accaataagt tcagctacaa gtactccaat tagtattgtc acagcaggtg
     7561 taaccaatat atctattttt gtttcttttg aaactaattt tccaacctct acagcaaaaa
     7621 gtgtagcgac aaatgctcca acaggtcctc ctaattcatt accacaagct ccaacaagtg
     7681 cacaagaaaa tagtacaagt ggaggtgctt gaagtccata cgcaatagcc actgctatag
     7741 ctggaccagt aacctgttta gcaagagggc ttataacttc agtaaaaaga gaaatattga
     7801 aattatctcc taatgtattg aaaattgttc caattaaaag ggacgaaaaa agaccgagtg
     7861 ccatataact tagtgcgtca ataagatacc tttgaacaga aatttcaata ttttttcttt
     7921 tagcaaatga tttgaaattt ctttttttat aattgatttc caatttaaaa tcctcctcat
     7981 ataatataag tataatttct ttttaattaa ttatataact tttcagctga caagacaaat
     8041 gttatttcac taaaattaga ctattgtaat agtaagaaat taacattaat tttagtttta
     8101 aattttaaat ttatggttta taattagtat agggatattc tgaaatataa taattttcca
     8161 gtttatccca atatttaact taggtagatt gcttacaaaa caaatgatta tatattaggc
     8221 taaaaggaag tggatagatg atatttgtaa aaaataagaa atacagaaaa attgccataa
     8281 ttatatctat tcctatattg cttgttgcgc agtactacct ttttaaattt ggaatactta
     8341 gaccaatggt aaagggagta gaaattgaaa ttgtagatgg ggaatatgtt aaagatatag
     8401 ataaatatgt agtaaaatta aatgaaacag ttactttgtc tacaggtgaa tatattaagg
     8461 ttccttcgta tgctaaaaat ccaaatattt ggtttaatgt attggataac tcagggactt
     8521 taaaaataga tggaaataaa atgacagcat taaaggttgg gtattcttct gtgggtatca
     8581 tgaaaaattc aagagtatta aaaaaggcta ctataaaagt agtagaccca gaggtagaat
     8641 cactagatat ggagattaat ggggatttaa aacatgttgg tgatagtgct actatagaaa
     8701 gtacaataga agttgattat gataagttta aaaaatcata taagccagtt tataagagta
     8761 gcaatgaaaa tattttaaaa atcaaaggaa ataaagtaag tgcagttgga gttggtaaag
     8821 ctacaatata tgctaagtgt ggaaataaag aaattgagag aacatttagg attcaagcta
     8881 gagttgctga aataaatatg gatgatataa aaatagaagt tgaccagaat gaaaaattga
     8941 aaccagatat aataacttct ccacctggtt tagaaccacc aaaaatatat tatgagttag
     9001 tagattcaaa attacctgta gaaagagcaa taagtttaag tagtaatgga aatgttgtag
     9061 ggcttagaga aggtaaagat agggtgagaa taacttgtgg agataaatct aaagtaataa
     9121 cagtaacagt agtcaaagaa actataacga atgactatat acaaaacttg gttggttcac
     9181 ataaaatagt aaacaataaa gttattatta aacttgaatg ggattatatg aaagatgtct
     9241 ttgattatga tatatatctt aaagataagt tgtcaggaga atcatttaag aaaattaaat
     9301 ctataactat aaatgaaagt gatttaggta agtctaacaa ggtatatgcg actatagaag
     9361 tagatatgaa gggtaatcaa aatatagatt ttgatattta tgtcataggt aaaaaggatt
     9421 cagatacaac taagccaagt aatgtaataa atattaccaa tggaaatgga aattcaaata
     9481 atgataataa tattaaagat atgagggtag aaaatatatc agctaatgtt gatagagata
     9541 ataatgtcat tagagttaac tggagtagta tatctcataa ggactgtact tacagtatat
     9601 atgtaaaaaa taatactaat ggagatggag aatttactct atatcaaagt gatatacagg
     9661 gaaatgagca tactataagt attcctgaag gagatttaaa tctagatatt tatgttgttg
     9721 caaattataa tggaaatagc tctcaaaata gtgatgttat aaatgtaaga tagatttgta
     9781 tacaaattga aagtgttgag tttatttaaa tattaaatta agaaaaagga tagtataggg
     9841 taattatact atcctttttt gttacaatat ataggtttaa aatctctagt aatataaatt
     9901 ataaaaagtc ttatttacac aaatctatag atattttttt tattaatcaa tattgtctta
     9961 aattgaactt agtgcagctt ctattgcatg tattactgtc gtatcaaata gtggaataga
    10021 agtgtcattt tgtttaatta aaagacctat ttctgtacat cctaatataa cagcctctgc
    10081 accttgtttg ccaagcttat ctataataga taagaaagct ttttttgaag attcagatat
    10141 aattcctaag caaagttcat taaatattgt gtcatttact attatacggt cttcttcatt
    10201 tggaattaag acttcaattc cattattaat aattacattt ttataaaaat cttgttccat
    10261 tgtatattta gtacctaata aggccacttt ttttatttct ttttccctta ataccgtagc
    10321 agttacatct gctatgtgaa gtaatggaat atgtatactt tcttgtattt tatctgatac
    10381 cttatgcata gtattagtac agataacaat aaaatctgcc cctgcctctt gtaatttaat
    10441 tgcagcatca gctaaaattt tggcgctttt ttcccaattt ccactagatt ggcattcttc
    10501 aatttcttga aaatctacac tgtaaagtat acatttagaa gagtgtagtc cacctaatct
    10561 ttcttttatt actgtattaa taacttgata atatgtaatg gtactttccc aactcatacc
    10621 accaattaag cctatagtct tcatattata cctctcttat ttatttattt tgaaatagct
    10681 atttacaaat acaagtatag catctaaaat agaagcatac aataataaaa ttgtatggag
    10741 tacaattata attaagagga gtaattatat gccaataaat tcttttgaaa actatcctat
    10801 gacatggaaa ccaaagttag ataaccgaaa accaccaata tataaaactt tagcaatgtt
    10861 attagaagaa gatattaaaa gaggaaattt aaatcctgga gataaattgc ctccacaacg
    10921 ggaattagca gattttctag atttaaactt aagtacaata acacgagcat ttaaactttg
    10981 cgaagaaaag ggactaatat gtgcaaaagt gggaaaggga acattcattt cttctgatgt
    11041 aaatgtaagt aatacattat tatatcaaac ggaatcaaaa gacataattg agttggggac
    11101 agttcatcct ccgtatgagc aaaatacata tattattgac tttattaaaa atgtactaaa
    11161 acaaccagaa atggataggt ttttacaata catgtctcca tctggcacat atatgcaaaa
    11221 aaagtcaatt gcaaaatgga tagaaagaaa taatgtatac accagtgaag aaaatatatt
    11281 attatctaca ggagggcaaa atgctatatg tgcaacttta ctaggattat ttaaagctgg
    11341 tgatagaatt gctacagatt ctttaagttt ttctggaatt aagtcaatag ctaaaatgat
    11401 aggtattcaa ttagtcccaa ttcctcaaga aaataatgaa atatcgattg aatatcttga
    11461 aagttattgt aaaaatgaaa atataaaagg catatatctt ataccagatt atcataatcc
    11521 aacgacacat actatgtctg atttatctcg taaaaagata gccaagatag caaagcaata
    11581 taatataata attattgaag atgctataaa tagcattttt agagatggta tacaaactcc
    11641 catattttct ttagctagtg ataatactat atatattttt agtacatcta aatttttatg
    11701 tgctggtctt agagtagcat ttgtagttgc tccaaaacga tatatagaaa atttagaaaa
    11761 tgcactatat aatatgaatc taatggtttc tccatttaca gcagaaatag tacaccgttt
    11821 attatattct cctattatag ataaaattat agaagagaaa agagatgcaa ttatagaaag
    11881 aaatgaagtt gctgataaag ttttgtcaga ttataatttg attggtgata agaattgcag
    11941 ttttcgttgg ttacttcttc ctgatgaatt ggatggaaaa tcatttgaaa tttctgcaaa
    12001 aaatctagga gttcaagtat attgtgctga acgattttca gtaggaaact ctacagttcc
    12061 aaaagcagtt cgtatttgtg taacagctcc aaaagatgta gaagaattag aaaaagggct
    12121 taatataata aagtcacttc ttaagagtta aatttatttt actattattt aattaaataa
    12181 ataaaaaggc ggagaaattt attataataa acctccgctt ttattattta atgaaatact
    12241 ttacttttta t
//
LOCUS       DAELJO010000117        11819 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELJO010000117 DAELJO010000000
VERSION     DAELJO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.117"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            291..968
                     /locus_tag="KPB88_002564"
     CDS             291..968
                     /locus_tag="KPB88_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054832.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1040..3055
                     /locus_tag="KPB88_002565"
     CDS             1040..3055
                     /locus_tag="KPB88_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0054833.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3105..3785
                     /locus_tag="KPB88_002566"
     CDS             3105..3785
                     /locus_tag="KPB88_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054834.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4302..4748
                     /locus_tag="KPB88_002567"
     CDS             4302..4748
                     /locus_tag="KPB88_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054835.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMQIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            5129..6046
                     /gene="pstC"
                     /locus_tag="KPB88_002568"
     CDS             5129..6046
                     /gene="pstC"
                     /locus_tag="KPB88_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG0054836.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            6048..6872
                     /gene="pstA"
                     /locus_tag="KPB88_002569"
     CDS             6048..6872
                     /gene="pstA"
                     /locus_tag="KPB88_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG0054837.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6891..7655
                     /gene="pstB"
                     /locus_tag="KPB88_002570"
     CDS             6891..7655
                     /gene="pstB"
                     /locus_tag="KPB88_002570"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG0054838.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7683..8336
                     /gene="phoU"
                     /locus_tag="KPB88_002571"
     CDS             7683..8336
                     /gene="phoU"
                     /locus_tag="KPB88_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG0054839.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8494..9363
                     /locus_tag="KPB88_002572"
     CDS             8494..9363
                     /locus_tag="KPB88_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0054840.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9450..10886
                     /locus_tag="KPB88_002573"
     CDS             9450..10886
                     /locus_tag="KPB88_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG0054841.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTNKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10934..11761
                     /locus_tag="KPB88_002574"
     CDS             10934..11761
                     /locus_tag="KPB88_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0054842.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         4634 a         1226 c         2119 g         3840 t
ORIGIN      
        1 actcaaacta gccaatagac aagtttctag aaaaataata aatttatacg tgaaaatgac
       61 tttaagctcc attctccaac aatcggagac tttaattagt atttaataat gaatcttaat
      121 gtttaataca atattcaaga attattcaaa aatacttaag tattatagtc tagtttgtaa
      181 agatattata aaataataat accagttata tatgaaaata aaacattata atttgtaata
      241 actataaaac ttggaattat aattaaaata cctttgaatg gagtggtgta atgtataatt
      301 tactggttgt agatgatgat gttgatattt taaacataaa tcaaatttat ttttctaata
      361 taggttttaa tgtatataca tcagagactg ctaaagatgc tatggatata gtagagagta
      421 tagttttaga ctgtataatt ttggatattt cattgccaga tatagatgga tataaaatgt
      481 gcaatgatat aaaagaaaag gttaacatac ctataatatt tttatctaat tatgagcatg
      541 aagaagaaaa agttcgggga tttttagctg ggggggatga ttatataaca aaaccttata
      601 gtttaaaaga acttgaactt agaatatatg ctcgtatgag gcagtacaaa aatattacat
      661 cagttcctaa tgtgttaaag ttttcagcgt tagtaataaa tgtaaattca agaaaagtaa
      721 cttttgatgg agaaagtata gatttaacta ctatggaatt tgaaatatta ttgtttctag
      781 ctgagaacaa agaacaagta ttttcaaaga tagagatata taaccaagtt tggaaaatgc
      841 ctgatattgg agatcaacat acagtgcagg ttcatattgc acagatgaga aaaaaaatta
      901 attctttaag cagggagcat caatacattc aaaccgtatg gggaaaagga tataagtttg
      961 taccataagt gagatactgt aattaataaa tagttcttgt tttatttaat acgactatga
     1021 aagagatgtg gttgatgcaa tgaaaaagta taatatacta aaaggaactc tcattgtatt
     1081 gatagtctct ctgttggcaa taacaggatt aaatcttctg tataaatatg ataataaata
     1141 tacatataag tctattagag caagtaatgg aattttaact atagataatg atactttcac
     1201 taaaggtaaa cttgtctttt tgatagatga ctgggagttt tataatcata aaatatttaa
     1261 accgaatgat tttacagagc aagatataaa accagaatat gtttttatag gtcaatatcc
     1321 agatttttct atgagaaaag ggactcagtc accatatggg aatgctactt atcgtatgag
     1381 aataaaaaat gaaggcaagg ataagctact aagtttagag cttcctgaga ttttttccgc
     1441 aagtgaagta tggataaatg gagaaatggt ttctaaatta ggagatgtag gaacagaaaa
     1501 atatagaccc aaaattaaaa attcagtagt aagttttatt gcaaagaaaa atacagaaat
     1561 tataatcaat gtatcgaatt tttcacatta ttatagtggt ttgtattatc cgcctgcatt
     1621 aggagaaaca aaggacataa gtaatatgat gttttatcga ttactttttt atagtataat
     1681 ttgcttcaca acttttgcaa tagctatatt ttcattgtct gtatggtttc tttcaaacaa
     1741 aagtaaaatt tacttatatt ttggatgtat gtgtgttttc tttgccatcc atgtatccta
     1801 tccctttgta catttgttag gactgccttt ggtaagcttt acatatgcgg ttgaagattt
     1861 gtcatatttt atggtagttt tatgtattat ttgtataaat ggaagtatat ccaaaataga
     1921 agaaaataaa gtgtatagat ttttaatttt accattagga attacaatga gtatcatatc
     1981 tatagtgata ccaattattc ttcttccata tgatatgtac tttgtcaaca tatatgggaa
     2041 gttaatagac atatataaat atattgtatt tatatatata ttaatgtctt cgctggtttc
     2101 aatatataaa aagacatgct ctgagcatat aagttcttat attttagttt cagtaaacac
     2161 tgtttttgga attagcatat tatttgatgc agttacgagt aatagatttg agcctatatt
     2221 tacagggtgg caaacagaat attgtgggtt tgtaattgtt attttatttt ctgttatgat
     2281 gataaacagg aatagaataa taataaaaga gaatgaaaaa cttacaagga atcttgaaca
     2341 tgaagttgaa gtgagaacta atgaacttac aacattgtta aatgaacgta aaagattctt
     2401 ggcagatgta gcacatgatt taaaggcacc tgtttcggct atacaagcat ttatagattt
     2461 gataaaggtt ggtaatattc atgtagatga agagacaaga acctatcttt atgctataaa
     2521 tcaaaagtca aatgaagtac aaaatagagt acgtagtttg caagaattta catcacaaga
     2581 taaaagcatt ggtattagtg aaaaaatatg tctgaataat tttattaaag agatatatga
     2641 aaataatttg ccagatacac aagcatgtgg tgtaaacttt gaagcttacg tattggagaa
     2701 aaaaatatat gcaaagataa ataaagagat gcttacaaga gcatttgaga atttaattta
     2761 taatgctttg acctttactc catttgatgg aaagataatt ttatcaatgg ataaagaaga
     2821 tggatttgca attattaaaa tttctgataa tggaaaaggt atacctccag aaaatatacc
     2881 aaaggtattt gacagattct ttacagaaag agacaataat gagccaaaag ggcaagggtt
     2941 agggctatat attgtcaaat ccattataag agagcatggt ggagaaatat ttgtagattc
     3001 agaactagga aaaggaactg tttttacaat aaaattaagt ctatgttttt tataataata
     3061 gcttatttaa tataaaagaa taatgatatt tgggagtgat tataatgcaa actaagatat
     3121 tgataataga tggagacaag gataattgcc agaagctaaa aggatttctg gaggaaaaag
     3181 gaataagtat agatttagca tataattgtg aagaagctat tggaaaaata tttagcaata
     3241 aatatgattt aatattttta gaaataattc taagtgatgg tgatggatgg actctttgta
     3301 aaaaaattag aaatgtaact acttgtccaa ttgtttatat gacttatata aatgaagacc
     3361 aaagcatttt aaatgcttta aattctggtg gagacgatta cttaataaag cctctaaatt
     3421 tagaaatatt atatgcaaaa gtaaaagcta ttttaagaag aatgaactca tatgtaaata
     3481 ataatgaaaa taatacaaaa ttagaggaat ataatagaac tagtggagta ataaagttag
     3541 aagataaaat aataaaattg actccaacag aaaataagtt gttaaacttt tttatagaga
     3601 atcctgaaaa gactttgact actaaagaga tatatgaaca tgtatggatg aatgaatact
     3661 tagaggataa ctatagtgtg gtagttgcag tgaatggatt aagaaagaaa atagagaaag
     3721 attataaaaa tcctcagaaa attataacaa taagaggagc aggatattat tttaataagg
     3781 aataataaat atattaatat cattaataat tgttataaat gtgaaatgct aacgatatct
     3841 aaactaaatt attataaaat agctgactgt aatttaattg ataaagccta ttcttctaga
     3901 ctttgccagt taaattacag tcagttttat tatataaaaa aatgcgtaac ggaactatta
     3961 ttttatagat tatatttgaa aagataaata aattgtatat ctaaatttat atattatttt
     4021 ttacctttaa tatatattaa ataataaaat agttaaataa taagattaag aatttcttaa
     4081 taaatccaga agatgctttc gattatataa tattttatat ttttatatta ttaattatat
     4141 atgttgcaat tgctgcacct actttttctg gtgagtatca gacacaatca gatagcatcc
     4201 ttcgttgtac gaaacatgga catattcgtc tagcgtttac taaaatatta gctctatttt
     4261 ctatatttat aataatgttt gttatatgta tttctatcca tttggcaatt tctgatttag
     4321 cttttggtac tgaatgtttg aaaacctcat ttcaaatgtt gttttctgtc attagtctag
     4381 taaatataaa tttactacaa gcacagattc ttatagctat tggtggtcta atttcaatac
     4441 ttgcaatggt tagtttaaca ttatttttat ctgcaaaatg taaggattct cttacttcta
     4501 tgcagattgc tttttctatt tgcttaattc ctatgggaat aggtgttagt tggattggat
     4561 acctattacc accaggggga attggtttta agattagttt attatatcaa atgacatcat
     4621 ttaatttctt acacttagga ggtatgagtt tttggacacc acacattata ctgtttttct
     4681 cagtgataga aatacctatt tttatatttt tggctattcg tacctattgc aaacatcaag
     4741 tatcgtaaca atttgatgga aataatagtg atataaacta gaggatacaa taattatata
     4801 tgtagtataa aagttaaatt tagttaacaa atcttaatag cagatatagg taataaatta
     4861 tacataaact gtattattat ctatatctgc tattttaata tagagaaaca acagtatatt
     4921 gtaaagtaaa tgtcaaatat agttttaaaa gtaaaattaa tgttaaaaag taaatataat
     4981 tttacacttg cttaacttaa tcctaatata gatttaactt aaaaaaagta gaataaaagt
     5041 ataggaaatt tgatgaagac gaattataaa aaattaaatt ttgaatacag aacgaaaatc
     5101 gataagtata ttttaatggg agggtattat gatagtaaaa tctagattag taggtttaga
     5161 aaaaaatgac aataatggca ataagacaaa atacgtaatt gaaaaagtag caaaaaatat
     5221 ttttctactt agtgctttag tagcagttgc aagtttactt ttgataatag gattcgtatt
     5281 ttataaagga ttaagaccat ttatatttga aggatattca tttatagact ttttaactgg
     5341 tgcagattgg gttccttctg ccaataaatt tggaatatca tctatgatag tagcatcaat
     5401 atttgcaaca cttggagcac ttgttatagg agtacctgtt ggtatattaa cctctgtatt
     5461 tatagcagaa gtagcaccta aaagattagc aaaaataatg tcaccagcag ttgaactttt
     5521 agcaggtata ccttcagtat tatatggtgt atttggtctt gcagtgatag ttccaactat
     5581 acaaagtata tttaatcttc caaaaggtca aagtttgtta gcaatagtaa tagtattagc
     5641 agtaatgatg ttaccaacaa taatatctgt atcagaaaca gcaataagag cagtaccaaa
     5701 agcatacaaa gaaggttcat tagcacttgg tgcatcaaaa atagaaacaa tattcaaagt
     5761 tgtattacct gctgcaaaat ctggagtatt agccgcagta gtgttaggag taggaagagc
     5821 tttaggtgaa acaatggcag taatacttgt tgcaggcaac tcaccagtta tgccaactgc
     5881 aataacagat agtgtaagac cacttacaac taatatagca cttgaaatgg gatacgcatt
     5941 tggtacacat caagaaatgc tatttgcaac tggagtagta ttgtttacat ttatattaat
     6001 tttaaactta gttttaaata aactttcaaa taaggcggta aattaagatg agaaagttca
     6061 aagagaatct attgaagtca ttagtatatc tatcagcact atttacaata gtatcattag
     6121 tagttatagt gggatttata tttataaagg gaattggcaa catgaatttt aatttcttat
     6181 ttagcaatta ttcagcatca ggtgatggtg gtatacttcc aatgataatt acaacattat
     6241 atacagtagt agtttctata gttgtagcaa ctcctatagg aatattggct gctatatatt
     6301 tacaagaata tgctaaaaaa ggaaaagcag ttacagctat aagatttgca actgaaagtt
     6361 tatcagggat accatctata gtatatggat tatttggagg aattttcttt gtagttacat
     6421 taaaaatggg atattccata gttgcaggtt cacttacagt tgcaataata atattaccag
     6481 taataattcg tacaacagaa gaagctttaa aaacagttcc tcaagcttat agagaatcat
     6541 cattggcatt aggagctaca aaatttcaaa ctttatataa ggtagtactt ccaagtgcaa
     6601 taccaggaat tttatctgga gtaatcttat cagttggtcg tataattgga gaatcagctg
     6661 caatattact tacagcaggt actgtagcca agatgccagg tggaatattt gatagtgcaa
     6721 gaacattaac tgtacactca tacttactga ctaaagagag tggagatata gcaacagcag
     6781 ccagtatagg aattgtttta atagtaattg tgttagcact aaacatgcta gcaagatttg
     6841 ttgctaagaa attaaataaa gctaattatt aaatttaaga ggtgtgaata atggaattaa
     6901 tcgataagat aaaaatgagt gtaaaagatt tggatttatt ttatggtgat aagcaagcat
     6961 taaagaaaat aaatatggat ataaaagaga ataaggttac tgctttaata ggtccatctg
     7021 gatgcggaaa atcaactttt ataagaacgt taaatagaat gaatgactta atcgaagatg
     7081 taacaattaa aggaaacatt tctgttgatg gagaggacat atatacctca gatgatgtta
     7141 taaatcttcg tactaaagta ggaatggtat ttcaaaagcc aaatccattt cctatgagta
     7201 tatatgataa tgtggcatat ggtcctcgta cacatggact aagagataag aagcaattag
     7261 ataaaattgt agaagaaagc ttaaaaggtg cagctatttg ggatgaggtt aaagacaggc
     7321 ttaaatcatc tgcactagga ctttctggtg gtcaacaaca gcgtatatgt atagcaagag
     7381 caattgctat gagaccagaa gttattctta tggatgaacc tacatctgct ttagacccaa
     7441 tttcaacatt aaaagtagag gaacttatag aggatttaaa aaaagactac acaatagtca
     7501 tagttacaca taatatgcaa caagctgctc gtatatcaga tgaaacagct tttttcttaa
     7561 atggagaggt tatagaattt agcgatacaa aaactatgtt taccactcct gtagataaaa
     7621 gaacagaaga ctatataaca ggtagatttg gttaaaatat ataactataa aggagttaga
     7681 caatggtcaa taatagctta gatataagta ttgatacttt aaaagaacaa acagtgctta
     7741 tgatggaaaa atgtgaagaa gcaatagaaa agtctgtgac ttgtatgata gagaaggatt
     7801 tagagggtgc aagaaaagta gtagaagatg atgaagaaat aaataaatta agagaagaaa
     7861 taagagataa gagtatagag ctaatagtat taaagcaacc aatggcaagg gacttaagat
     7921 ttatttattc attaggtaat atatctatag agctggaaag aataggagat tatgcagcaa
     7981 atatagctag agaaatgttg aagataggtg gagaggctta tataaaagag ctaatagata
     8041 ttcctaagat ggcaaaagaa tgtaagtcta tgatgtctca gactagagaa gctttaatct
     8101 tatttgatgc aagtatagct tatgatgttg gaactaaaga tgatattgta gatggattgt
     8161 atgaacaagt acaaattgat tgtttaaaaa taatgcatga aaactcaaat accatagacc
     8221 aaggggtaaa acttctactt ataggaagat accttgaaag aattggagac catataacaa
     8281 atgtatgtga gaagataatt tttgcaattg agggtaaaat ggttgagata ggataaaaaa
     8341 ctgctttaaa gcagtttttt tattgtctaa attaagaaag aattgataaa aaaatatgat
     8401 taatgggtaa atttaaaaaa ttgtgatata ctaggaacat attaaatatg aggatgcgta
     8461 ttttatataa ggtgcttttt aggaggtgga actttgaaac ttcaaaagat gggtatgagg
     8521 actataaaaa caggaattag tgtcacttta tgtatgattc ttggtgacta tttagtagag
     8581 aatatgtttt attctctggt agcatgtata atttctgttc aagatacagt aaaaggttct
     8641 ttaaagacgg gaatcaatag agtggaaggt actattttag gtggtataat aggattcata
     8701 tttgccatca ttaaagctgg agacccattt ttatgtggtc taggagtaat gtgctcaata
     8761 tatttatgca atctgtttaa aataaagtca gtagtagttg cctgtgtaac ctttctggct
     8821 atacatctag gtgtaggaaa tagtgaccct gtatattatt ctttacatag ggtgttagat
     8881 acatctgtgg gtgttttgtt tggagtactt gttaattatt atgttgctag accaaattat
     8941 ctaaatagtg ttatagatga gtttaaaaag atagaagaac tttcaatgtt gcttattgaa
     9001 gataaaattc ttagaaaaga aaatttaaat gtagagaaat ttgaagaaga agtagaaaaa
     9061 ttagaagaaa tatattcaaa gcttattgat gagttggatt atagtttgag tgaaactaat
     9121 atagaaaaaa ttgagaatgc cttaaggata tgtagagaaa tacattttca tatgcagtct
     9181 atagagttac ttgaaaataa actttatttg aaagagcata catataaata ccttaagaaa
     9241 acatataatg tggataagct agactggaat atagatgaag aaaaaagccc tgtattcaac
     9301 tatcatctaa tgaaaataat gattgaaata gagttgttga gaaagctaaa taaatcatat
     9361 taataatagt agttaaataa taaataaaaa atagtatctt tgtaaatatt aaacatatat
     9421 aagtttaatt tatggaggta ctatttttta tgaatactag aaatgacaat tctaaaatac
     9481 tagaaatata taaaatgaag ctttttggcg aacttgtaaa agcagtgtgg aatgatgata
     9541 tagaggatat tgaaaactta cctaataaaa ttttaaatga agatttaaag gataatttaa
     9601 ataattattt tagtggaaaa tttgataaag atactatggt aaatttaatt agggtaacta
     9661 tgggattgga tgtgacagaa gatacaaata aaaatttaaa agagtcacta catgaagctg
     9721 taaatttaga tgaagtcaaa agaccaataa tatctgtaat atcagatgct tgcaaatatt
     9781 gtgatgaaaa taataaggaa gatgagtcat gtaagataag aagtaagcat attaattgta
     9841 atgaggaaga tgtatgtagt gcttgtggtg attgtgtatc aagatgtaag ttgggtgcaa
     9901 tttcagataa aatagaattt ataccaatga taaaattgtt aaaagatata aagagccctg
     9961 tatatgcaat agttgcacca gcttttgtag gacagtttgg gaaagatgta agccctggta
    10021 aattaagaac tgcattaaaa aagataggat ttgaagatat gatagaagtt gctttggcag
    10081 cagatatgct tactgcaaaa gaagcatatg attattatga gcatatgaaa aaggacgaag
    10141 aaggatgctt tataactagt tgttgctgtc ctatttgggt tagtttgata caaaatagtt
    10201 ttcctcagat attggaaaat gtatcaccat ccgtttctcc tatgattgct tgtgggagag
    10261 ctgtaaaaat tttaaatcca gatgctaaag ttgtattcat aggtccttgt atggcaaaaa
    10321 agaaagaggc aatgttagaa gatataaaag atgcagttga ttttgtgctt acatttaaag
    10381 agttgcaaga aatatttgat gcattagagt taaatccagc aataatggat gagagtaata
    10441 gagtagaggc gtccctttca ggtagagttt atgctagaac aggaggtgta agtaaagcag
    10501 ttaagttatc agttagaaga attgacaaaa atataaaatt tgaagaaaag gcatttgaag
    10561 gaacaaaaga gtgcatggac ggtctaaaga aagttctaaa taaagaaata gaagctacat
    10621 ttatagaagg aatggggtgt gtaggaggct gtgtaggagg tccgaagcgt attttaagtg
    10681 tggaagaagg tactaagaat gttgatgaat atagtgaaag tacgaatatg gaaactccat
    10741 ttgataattt aaatgtatta caatttttaa cttctttggg aatcaaaaga gtagaatctt
    10801 taggagaaaa agaagaagaa aaggttaaaa aaatatttag tagagatata aaaagtaata
    10861 agaaagagaa taaatataaa aaataacttt ttaagtctta aacttatgct tttataaaag
    10921 ggagatatgg gggatgaaaa ttaaagataa taaggctgtt tttcatgtat ttataaggat
    10981 atttcttata gctgttttaa ttttaactgg aacattattg gaggaaaaaa ttaataatgt
    11041 tccagttgaa aatataagtt caaaaggtga aaatacactg gaactgcttt ctcaaagtaa
    11101 tactttagga gaaatcatat acaaagcatc tcaagaagaa acaaaaggtc caggggctac
    11161 ttctgaaaaa gtagcataca taacaataga tgatggtcca tcaaaatata caaatgcact
    11221 acttgatatt ttaaaagaaa atgatgtcaa agcgacattt ttcatgctta atcagaatat
    11281 gaaaaaccac aaggaagaag taaggagagt cttggaagaa gaaaactcaa taggttttca
    11341 tagtgtaagt catgatatac ataagttata taaaagccca gaagtaacag ttggggaatt
    11401 taatacatgc aataatactc tacaagaaat aactggtcag agttcaaaat taataagatt
    11461 accatacgga agtaaaccat atatgccaga aggttcatat aataaactta tggcaaatga
    11521 ttatttaatt tgggactgga atttggatac acaggattgg aagtcttcta caagtcagat
    11581 agtaagtaat atattatatt atggaagaaa aagagatgaa ttagtagtat taattcatga
    11641 gaaagagcaa agtttaaatg ctttaaataa tattataaga atcttaaaag agagaggata
    11701 tacaatattg ccaattacag aaaatataac acctaaaaat ttctggcaag aaaacttata
    11761 ataaatttaa atatagtaaa agataaagta aattatttat aaaataaaac atataatat
//
LOCUS       DAELJO010000118        11776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELJO010000118 DAELJO010000000
VERSION     DAELJO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.118"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            253..2862
                     /locus_tag="KPB88_002575"
     CDS             253..2862
                     /locus_tag="KPB88_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0054843.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKRNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYERFERPIEAILFLANKYNISLPINKS
                     KTNIKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(3048..3296)
                     /locus_tag="KPB88_002576"
     CDS             complement(3048..3296)
                     /locus_tag="KPB88_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054844.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(3322..4050)
                     /locus_tag="KPB88_002577"
     CDS             complement(3322..4050)
                     /locus_tag="KPB88_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054845.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            4186..4731
                     /locus_tag="KPB88_002578"
     CDS             4186..4731
                     /locus_tag="KPB88_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0054846.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            4724..5425
                     /locus_tag="KPB88_002579"
     CDS             4724..5425
                     /locus_tag="KPB88_002579"
                     /inference="COORDINATES: protein motif:HMM:NF025643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBG0054847.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            5494..5931
                     /locus_tag="KPB88_002580"
     CDS             5494..5931
                     /locus_tag="KPB88_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054848.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(6079..6411)
                     /locus_tag="KPB88_002581"
     CDS             complement(6079..6411)
                     /locus_tag="KPB88_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0054849.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            6527..6895
                     /locus_tag="KPB88_002582"
     CDS             6527..6895
                     /locus_tag="KPB88_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054850.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            7485..8120
                     /locus_tag="KPB88_002583"
     CDS             7485..8120
                     /locus_tag="KPB88_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0054851.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYKDVITGATKER
                     EALSRMLVEVNKGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTNNNT
                     YKMLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKIYEGGEIKRNRS"
     gene            8476..8724
                     /locus_tag="KPB88_002584"
     CDS             8476..8724
                     /locus_tag="KPB88_002584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054852.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNEPGVFRVKESDEQLLY
                     DCLEETQNIRQEKHIAYAIRFRGKSVYCVFYESPFWDL"
     gene            8740..9285
                     /locus_tag="KPB88_002585"
     CDS             8740..9285
                     /locus_tag="KPB88_002585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0054853.1"
                     /translation="MRVEPIENKKDIEKLKRYFKNKGKIRDLTLITLGLNIGFRIEDI
                     LKLKVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADDAIMKLNL"
     gene            complement(10400..10945)
                     /locus_tag="KPB88_002586"
     CDS             complement(10400..10945)
                     /locus_tag="KPB88_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054854.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
     gene            complement(11025..11228)
                     /locus_tag="KPB88_002587"
     CDS             complement(11025..11228)
                     /locus_tag="KPB88_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0054855.1"
                     /translation="MDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTNSIKFD
                     SLEKICMALKCSPNELFGWEQDK"
     gene            11397..11699
                     /locus_tag="KPB88_002588"
     CDS             11397..11699
                     /locus_tag="KPB88_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054856.1"
                     /translation="MLKDDLLRIGFDTYGVNLEDKLLENNEIYLNDKKESERIMNRLL
                     EILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFIDLNKKLCLKRN"
BASE COUNT         4774 a         1281 c         1821 g         3900 t
ORIGIN      
        1 aaaaaaaacg aaaaagaacg agtatttgtt tgacggcatc cactcattct ttccatttaa
       61 gggttaatca agattaggag ttagccacta ggggttaata ctaggagcta atcacttagg
      121 cttaattaac atataaaata tatatttcct tattctttta tatgttaatt atacgagatt
      181 tacggcagaa tgtaaataca tagcttgata aaacttgatt agcccctaac taaaaaaggg
      241 ggatttttac aaatgaaaat agatataaaa caattaattg atataataaa aaatgccaat
      301 atagatgaac tatatttaag agatatagga gtcgatacaa ctcaaaaaag gaatgttcct
      361 tgcccaattc atggtggtaa ggataaaaac ttccaattcg atttaaacaa aagaatatat
      421 acatgctata gccataattg tgttgttggt gctgatataa ttgaattatg tcgcacatat
      481 gaacgctttg aaagacctat agaagctatt ttattcctag ccaataaata taatatctca
      541 ttaccaatta acaagagtaa gacaaatatt aaaaaaacaa ataatactat taaaaacatt
      601 tacataaaga aaaaagatat aaatagcttc atagaagaaa agaaacaaga agcttttaaa
      661 aacaatgata ttgagttagc ttttgaattt gagtgtatga ctgataagga caaaagaaag
      721 tattacttag ataacaatat taaaataaaa tccatgctag aaaaacaaaa ctattcaagt
      781 tataaagctg attcgataat taatatagat aaatacataa gtgaaaatct aaaaggtatt
      841 agagagtcaa ttaaacatgc tacagaaggt aagaacgtat taatggtagc tcctacagga
      901 agtggaaaaa catactcaat tataaataca ttaaaagagc tggatataaa agcactcttt
      961 atattcccca actctgcgaa tgtgcaacaa gcaatagtcg aatataaaat agctggtgca
     1021 tatgataaga tacctactaa acacgcttta gagggaaaca agttagtggc tatgacgtgg
     1081 gataagactg aacagcttct aaaagaggat ttgagcgagt acataatagt tattgatgaa
     1141 atacatcaga cttacacaga tgcgttcaga agcaaagcaa taaagagctt aaataatata
     1201 actaataagt gcagaggtag actagatata actgcaacac cctctaaatt agaatttgag
     1261 atatataaca aagttattga atataaacaa aatatacaaa cggaatacaa agttaagtta
     1321 tacgataaaa tcaatattgg gaaagtaata gagatactaa ataattcaaa taattcaatg
     1381 cttttaaaag atagtatatc aacacttaac tatatatcaa agaaagttaa taaaaagagt
     1441 ggagttgtta tttcagatac taaagacact agcaaactat atgatagaat tgttagttac
     1501 tccgatatgg aaggctatga ggtgctttta aatacgtctg taatcacggc aggtgtaaac
     1561 ataaataatc ctaatgtaac agatattatt gtaattggag aaaaggatgt aggaaaaata
     1621 aaacagtatg ttgcaagagc tagaggggca aaaagtataa atgtacatat ttttaatagt
     1681 tacaaaacga caaatgagga tagcaatgta tattcggttg aatggtgcat aaatgagaac
     1741 ataaaagacg ttgagagcct aacaaatata tacaataaag cttctaagag agacttacca
     1801 tatagagcta taggaattga tataagccct ataaacataa aaaacattga tagtaatata
     1861 tactatgata aaaaagatat gtgctataaa acggacaagt tatacataaa aagtaatgct
     1921 tacaataact attaccaaac aagaacaata aacagcttca aagtgctatt agaggaatat
     1981 tttaataaaa ttgagatagt agaaactgaa aaagaaacta aaaaaataga ggaagaaata
     2041 aaagagcatg aggaagctat taaggaattt aagaaatcag ctatagaaca actagaagga
     2101 cataaaaagt atctagttgg ctatagtgaa attaagaagt caatgaccag cagtaactta
     2161 ttaaaatatc atcatgacat gaggatagat aataatatct gtttaaaata ttatatggaa
     2221 catgatttat atagcctaat aattaataat aattgtagaa aaacgataga cttatttagt
     2281 gattttgtat tagataatag ttatagtatt gacctatctt ggaaacttgc gacaatgtca
     2341 gacgagaaaa gaaatctatt ttttgaacag ataaatacaa caatttatcg aaaaattaat
     2401 aaaaaatacg caaattcttt gctaaatgat gataatattg gtacttatac ttacaattat
     2461 ataaacttca attttggagt tggtacaagt tatacaaaag aacacttaga agctctagcc
     2521 gttgatttac agacgttttt agcaactaag aaaaagctaa ctattaagaa aataagtcta
     2581 atattacaat caatttttgt tattgaagtc aaacaacaca ggggtgtcac agggttgccc
     2641 tataaatact ataaaaatat acttccaaac cctgtgacag ataaaaaagt aattagagtt
     2701 tacactatta agaaacatat agacatagag gacataaaaa aagaattagg actaatagac
     2761 aatgatttaa gcatagagca tttagttgaa gctagaataa ataaactttt aaatgcgatt
     2821 gatgaaacgg aaaaagaggt tcttttggaa ggattaatct aatagctaat cttaagtacg
     2881 aatagtacta aaaaatgttg ctttgtatta agggggacga agtcctgttc tctttttgag
     2941 gtcgagtttt gggtgttgta taaataaagg ttaaatcatg aaaggttgaa agtagcttat
     3001 aagtgtaaaa taaaaggact tataatttat aagtcctctt attttattta ttttttatta
     3061 atttgtttca gtttggttaa gttatagatt cctatggaaa atagtacacc acctattatt
     3121 aaaaacatat aggaatagtt ttttaatatt tgattttcaa ctatgtcttc gaagccttta
     3181 tcagctaagt atgttgcttt tagtgataat gctaagtcat tattaaaaaa taatattata
     3241 aagcctaata ctgctaatat taaactaacc cttaagtaca tatttttatt ttccatttta
     3301 atcaactctt tctattattt attatttgta tgcaagatat tctaacccat cacaaacttt
     3361 tcctgcttta agaggacttt ttgctactat tttttgagtt atagaagttc ctgcaagtat
     3421 cccatttact gtacctctta cttctttata atatggttta aatgctacat atcctatttt
     3481 acctgcaggt actttgtgtg tagttgtgac acttcttgtt aaagatttgc tccatgtaaa
     3541 tcctgcattt gctcttactg ctgcctttat tgcttctgta gttccaccaa cagtaaaact
     3601 ttcagttaca gttgttgaat ttcctataga tatagtagca ccttgttttc cacctgatac
     3661 gtcttgtgat attttgcgtg cagaaccagt aattttttta acacttgata ctccaaaaat
     3721 agacttagga gattttgtga taatttcctc atttaaatta aatttttcta gtgcttcatt
     3781 aatacttaat ttaaccaatt ttccatttac attttcatat acttcagaaa ctttttcgtt
     3841 agtagtttta tttaataata tttcttttcc tgtttcagta tcgaatgatt taacccactc
     3901 ttcttgctgt ttagattcat tattatatgt atattctgtt cctaaccctg ttgattccat
     3961 tgcatatgat gttgctgtta ctgctggtat tgctagcatt gccattgtca ttgtagttgc
     4021 taacattttg tttattcttt ttgatttcat ataaaacacc atccttttat gtttttattt
     4081 atatatataa caaatgaagt ttttattttt agacatttat atataatttt ttataaattg
     4141 tatataattt ttttaaggat aatatttttt aagggagaat aatttatgtt aatatattta
     4201 gctattatag attcaaaaga agaaagaatt aaatttgaag aaatatatat taaatataag
     4261 aaattaatgt tttatgtagc taatcaaatt ttaaaagatg atgttttagc ggaggatgct
     4321 gttcatagtt catttttaaa aataattgat aatcttgata aagtaaatga tgtgaatagt
     4381 ccaaaaacta aaggttttgt tgttattata gtaaaaagga tttcaataaa tatatataat
     4441 agaagaaaaa aagaggaaat aagtaatatt aatgatgata actataaatt cagctcttta
     4501 gatttatcga ttgaaaagtt aggtgagtgt agttatttag gagaagcttt aaaaaaactg
     4561 tcagagacag atttacaggt gattctctta aaatatagtc atggtttttc tatcagagaa
     4621 atttctaaac tactgaatat aaaagaggtt aatttgtata agagaaataa aagagcatta
     4681 gaaaaattaa aaaaaatttt aagcgaaatg gaggcattat caaatgaata aagataaatt
     4741 agacatagaa aaattattat acgaacagct tccaaaaata gaagaaaaaa tactgtatga
     4801 attaccttgt gatgaagata ttgactatga attttctata gaatttgaaa gtaaaatgga
     4861 taaaattata aaagaaaatg agaaaaaagt aaaaaagaac acctataaaa acttaaaaaa
     4921 aattgctatt gtttttataa taattttatc aagtatagtc actataacta ctataaaaac
     4981 agaagctttt ccagaaaatt taattagaat aatgaagaag gtatataaag aatttacaga
     5041 ctataatttt ataaaccctc ataaatcaaa tgtatcattt aaatatatag aacctaaata
     5101 catttctact ggatataaag aagttgagag aattgaggaa gaaaattatc tatcaatatt
     5161 atataaagat aaattagata actatataat ttatcaaatg atgttatatt ctagggatgt
     5221 tatagtcttg gatacagagg atgcttttgt tgagaatata aaatttaata atcagtatgc
     5281 acagtatgtt gaaaaaggaa atactcaaca attattgtgg catgataaaa ataatattta
     5341 tatgttatct aaagagctac ctacaaaaaa aataactaag aaagataaaa aagaatttat
     5401 taaaattgca aaaaatatta agtaaatttt tgtctaaaat agtgctagtt agttgttata
     5461 ttgaatatat aagattttat aaggaggtac ttaatgagag taaaaaatat aatctcaata
     5521 tttgtatgtg cgttgataat aataatgtca gtaaatgttg ttagttttgc tgaacagttt
     5581 aattcatctg atattatttt tatggaagat ggtaaagaag aaataaatcc tttttcagtt
     5641 tacatattaa atggtagatg taatttatca ttttctaatg gaaaagcaaa tgtaaatgct
     5701 tctgttgttg gaaaatctgg agtaactaga acatctattg taactaagtt acaagaatta
     5761 aaaggcggaa gttgggtaaa tttaaaaaca tctacaaaga aaactggagg aaaaacttgt
     5821 tctgttttaa actcatattc tgtgacaaaa ggacatactt atagagctgt tgcaataatt
     5881 actgctgaaa atgagtcaaa aacattaata agtacatctc aaaaatatta aaaaaagaaa
     5941 atacacttac aagagttaag aaacgttttg aaaactacta cagaagtatc taaatcaatc
     6001 aaaattagat agatatagga acattataga aaaacttaaa tagtacatag gtataataat
     6061 ataatttata ctcatgtact attttttatg tgtaatattg caatctttca aagagtttaa
     6121 aaggctttcc aatgaatttg aatgtatgtc atttagaaat ctttttgttt cagatatttt
     6181 ataatctttt tttgctatga gtatcgtata atgtgtcatt ctgcctattc gttcagatgc
     6241 taaaaagtgt ttaataacaa gccttgataa gattgttagg gttgttgtca ttttccaact
     6301 atattcaagt tccatagctt ttgcaacttc tttagatgtt acaatgtcgt ttttgctcca
     6361 aataaatttc atgactttga gttctgtttg aggtatcttt ggtatatcca tataattcct
     6421 ttctatacac tataactatc ataatgaaac attctattaa tttattataa tatactttgg
     6481 gaaaggttac aatatgtgtg aatacttgta ggaagggaat acgcatatgg agcttgattt
     6541 caaatttata ggtcaaagga taaaaattgc aagaatcaag aaaaagctaa cacaagaagt
     6601 tttatcagaa aaaatcaatg ttagtccaca acatgtcagc aatattgaaa cgggaaattc
     6661 aagtgtaagt ttgccaacgc tcgttgctat tgcaaatact cttggggtgt cggttgatga
     6721 attattatgt gatacaatct taagttctaa agctgtcttt gaaaatgagt tagaagaagt
     6781 tttgagggat tgtaatgagt atgaaataag atttataact gatattgtaa aagcttctaa
     6841 aaaatccatt agagatataa aactgtttca atcaaattta aatgatagat attaatataa
     6901 atatatgctt caataattta attattattg atgtttttat tatatttaaa taaaagttgt
     6961 ataaacttat gagttaataa ctgaaaatat gactaatttc catattttac tatcaacaaa
     7021 aaaagtataa atgtagaaaa aatacggtat ttttgttatt atttaagtat aaaccctatt
     7081 acaaaacaga tttgataaaa cgtatgtgat gattatgtaa ttatctctat ctacctctta
     7141 tgaggttctt aaatggtata tcattaagaa ttatattgtg tgaaaacata attcttactg
     7201 atataccatt tttgttttaa aaacttggtt gcctaaaaaa tataaaaaga ttttggcgat
     7261 tttatataaa atatttaagg aatagttgat ataaaaaagg tgatatgttc gtgttcggaa
     7321 aaaccaacgt ttgaccgtac acacaatatt tgtatgattt ttacatagaa tcattggaaa
     7381 tcaacaataa aatatgttcg gcattgcaaa aatgaaaaat atagtttgac cgaacaaaaa
     7441 atatattgta taattattat aacaagacaa atgggggtag ggttatgata ataggatatg
     7501 aaagggtaag tacaagaaaa caagataaca gcagacaaga gagagtttta gagaatgagg
     7561 gaattaagtt cgataaaata tataaagatg taataacagg agcaacaaag gaaagagaag
     7621 cattaagcag aatgttagta gaagtaaata agggggatac tgtttactgt gagtctataa
     7681 gccgtttagg aaggaattta aaagatttaa ttgatattat agatacatta gttcaaaaag
     7741 gtgtaagagt aaaaatatta aaagaaggta tagacacaaa taataataca tacaagatgt
     7801 tacttggtat atttggggca gttgcagaaa tggagagaga aacaattcaa gagagaacac
     7861 agcagaaaat agaccaacta aaagaggata agaaaaatac tggcgagata aatacaaaga
     7921 gtggaaaatg gttcggtaga caaaaagtaa cgaaggagct tttagaaact aacaaagagt
     7981 ttataaaata ttataataaa atccttgatg gaagcttaaa gaagaatgaa gtggcaaaaa
     8041 tattaaatgt tggtaggact acactttacg agtggataaa gatatatgaa ggtggagaaa
     8101 taaaaaggaa tagaagctaa ttgattataa tatatttaag tgttattatt ttgaacaaaa
     8161 attgaacaaa attagacaaa ataagaacaa aatatatagt ataattatat tattgaaagc
     8221 tttacaattt aatagctttt gcaaacaaga gtctaatttt ttaggctctt ttttttatta
     8281 taaatattaa attttgattt acttaagaaa ataagatttt ttttggtata gtagatagaa
     8341 tgataaaatc acatcaaatg attttgtaaa ttaacaagta ataaaaaact acatattttg
     8401 aacaaaatag agacttttgg attgtaaaaa tatattaaaa taatttatat aaatagaaaa
     8461 atgagagggg aaattatgga aggaactaaa agaagttata ggaaaatacc aaaattagat
     8521 ttaagaaaag taaaattagc agatgtattt gaaaagaatg agccaggagt ttttagagta
     8581 aaagaaagtg atgaacaact cttatatgat tgtttagaag aaactcaaaa cattagacaa
     8641 gaaaagcaca ttgcttatgc gattagattt agaggaaaaa gtgtatattg tgttttttat
     8701 gagagtccat tttgggattt ataaatagga gtgagttaaa tgagagtaga acctattgaa
     8761 aataaaaaag atatagagaa gcttaaaaga tattttaaaa ataaaggtaa aataagagat
     8821 ttaaccctaa taactttagg gttaaatatt ggctttagga tagaggatat tttaaaacta
     8881 aaagttaaag atgtatacaa aaaaagagaa ataaatataa aagaaagtaa gacagggaaa
     8941 agaataagaa agcctataaa tgatagtttg aaagaaatat tagatatata ttgtaaagat
     9001 aaaccaacta atgaatactt atttaaaagt caaaagggag agaatgaagc aataagcacc
     9061 acacaagcat atagaattat taaaaaagct tcaaaagctt gtaggataaa attaaatata
     9121 ggtactcata gcatgaggaa aacacatggt ttacatatat ttaaggataa aggaattgaa
     9181 gtcgcacaaa gagctttaaa ccatgctaat caaatagata cattaactta tattggggta
     9241 gtacaaaaac aagctgatga tgccattatg aaacttaatt tatagttaaa aatgtatgat
     9301 tttctttgtt gtttttacat tttatagtta atgattaagt aaataagctt atgtataagt
     9361 aattgcaatg gataaagata actaaaataa atgtaatagt ttatagtaat cgtacatttt
     9421 tttatatact tctatctatt gctattactg gtatagatat tgtttttatc ttgtttagaa
     9481 aaaatatcta ttattaattg attattaaat gataatagat acatattgaa gcttaatatt
     9541 ataatatttc aaaattacta aactaagtga aaaaacaact cgaaaaacac ttttaattta
     9601 agtaaaatca atgcgtatgg ttattttttt tctgtatttt gaaataaagt tacaaactat
     9661 atattttatc ttattaaaat ttacttaatt tagattgaaa caattcaatt aagggtttga
     9721 aataaagtta taccaataca tgtaaggcta tttaatgcac tgaaaaaaag tataatgttt
     9781 agcccccctt gctgaaaata acgctgttga agaaaaatat gtgaaccgag gtaggtatat
     9841 ggcttcggag ataagtattg ttttgtgtaa ggtgtggggg tggggtgttg caacttaaaa
     9901 acatggttgc agatgcaaaa atacattaat aaagctatgt aaaatgttac tactaaagat
     9961 agtcggagtt gtttaaaatg ctatatttgg ctaataaagg atataaatat ttataactgg
    10021 ttaagtgttg ggaaataaag agcttgcata tgcaactgca cccctaaaaa gtatataggt
    10081 tgcaatgcct atatagcact tatagcacct ttaaaaagga atgatttaaa tattaatatc
    10141 aaaactaaag aagttagtaa ttgcaatgta ttaaggggca aattgtatat ataatcttaa
    10201 aataaataga aatataataa tcaaactaat gctatgtttt gatgagtata tgctcgatag
    10261 aaatatatat aatacgcccc ctatcggatt aataaagtaa ataaagatag aggactggaa
    10321 ggagtacata tttttcacag gaagccaaaa aatgagattt tcattgaaaa gtttttagaa
    10381 tatcagaaag taagtaaaac tacttatatt tatccataaa atccctaaga gcttggctga
    10441 taatatcttg taccctgtac ttcttattag cttcacagaa ggctacaaac tctttttgaa
    10501 tatcctcata gaccttatag cttctaacaa ctatattgcc tttaaaatcg tctatattta
    10561 gtttatcagc cccaactact ttattactac tttgtgattc gtaccaacta tatacttcat
    10621 ttagtttatt attcatatca ttgaatttag ataaaatgtt attaaaatca atattggttg
    10681 catcaactaa agtattgtca ttgctagtgt cgcccgcaac tacttttgaa ctactttcta
    10741 attcgacaac ctcaataata gatatatata gcttcttagt tggattaaat tcatatccat
    10801 gttgtttaaa tctatttctt atagttgtac gaccaatacc aatatcttta catgtgttag
    10861 ttaatgtatt gccctctaag agcttgttat taatatattg tatttgctcc ttgataggta
    10921 agttatcaaa tgtatatcta tccatgttta aacctccaaa ataaaataac tactaaagta
    10981 gttttctagt agttataaat aaacccttta ggatttatat ttttttattt gtcttgctcc
    11041 caaccgaaca gctcgttagg agaacatttt agagccatac aaattttttc taaagaatca
    11101 aatttaatag aatttgtatt attgttggct agtttagtta agttttgatg agtcattcca
    11161 atttgctttg ctaaccaata acgtgtttta ttttgatttt ccaataattt atttatttgt
    11221 atatccaaca atttaatcgc cacctttttt attatatagt atcggaaata tgtaccaaaa
    11281 acaatatata tgcttgacaa tacatagtaa ataattacat aataataaat agaatatatt
    11341 accatattta ggggtgcaaa agatttaagt gtgcataaaa aggagtgtta aaagtaatgt
    11401 taaaagatga tttattaaga attggatttg acacatacgg agtaaattta gaagataaac
    11461 tactagaaaa taacgaaata tatttaaatg acaaaaaaga atctgaaaga ataatgaaca
    11521 gattgttaga aatattggaa gaaaaagaac ataaaaaaat gctaattgat tttgaggaaa
    11581 aagtatttgg taacagtgta attgaaaaag aatacgcata ccgaatgggg gttttagatg
    11641 ggcttgacct agctgaattt atagacttaa ataaaaaatt atgcttgaaa agaaattaaa
    11701 aatatatatt aaaaccgtca gaaaaataat tctaaacaat gaattataac tttaatagtg
    11761 atataatact aataac
//
LOCUS       DAELJO010000119        11769 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELJO010000119 DAELJO010000000
VERSION     DAELJO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.119"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            128..1168
                     /locus_tag="KPB88_002589"
     CDS             128..1168
                     /locus_tag="KPB88_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054857.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1523..2845
                     /locus_tag="KPB88_002590"
     CDS             1523..2845
                     /locus_tag="KPB88_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0054858.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3142..4503
                     /locus_tag="KPB88_002591"
     CDS             3142..4503
                     /locus_tag="KPB88_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0054859.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4576..5970
                     /locus_tag="KPB88_002592"
     CDS             4576..5970
                     /locus_tag="KPB88_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0054860.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6032..6502
                     /locus_tag="KPB88_002593"
     CDS             6032..6502
                     /locus_tag="KPB88_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG0054861.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6505..8721
                     /locus_tag="KPB88_002594"
     CDS             6505..8721
                     /locus_tag="KPB88_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0054862.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8804..9475
                     /locus_tag="KPB88_002595"
     CDS             8804..9475
                     /locus_tag="KPB88_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0054863.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9838..11052
                     /gene="dpaL"
                     /locus_tag="KPB88_002596"
     CDS             9838..11052
                     /gene="dpaL"
                     /locus_tag="KPB88_002596"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0054864.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11179..>11769
                     /locus_tag="KPB88_002597"
     CDS             11179..>11769
                     /locus_tag="KPB88_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0054865.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4441 a         1352 c         2394 g         3582 t
ORIGIN      
        1 tactttttat taagttttgt tttaaaataa tattattaaa ttataactaa gcttttctgt
       61 tatgatataa ttaaatagat aatgtgaatg attattatat agaatactta tttggagttg
      121 atattttatg gacaaacaca gtgattcagt aatgtatggt ggaatggaag tatataatat
      181 ttggggaaca acaacagtag actccataat tggagagtct gatgtaatgc gtgctcttaa
      241 gaatagaatt tgtaaaattg gaaatagtga ttctactgta gctataactg gagaaagtgg
      301 cactggaaaa gaattaatag caagagccat tcatctcgca ggaaacagag ggaataaaac
      361 attcgttgct ataaactgtg ctgctatacc agaaccttta ctagaaagtg aactttttgg
      421 atatacaaaa ggagctttta gtggagcaga ttcaaaaggt aaaattggta agtttgaatt
      481 ggccaatgga ggggttgtat ttttagatga gataggagat atgccaattc aactacaatc
      541 aaagttattg agggtattac aggaaaagaa gtttacgaga ataggttcaa atgaactaat
      601 tgatatagat gttagaatca tatcagctac taatagagat ttatttgagt tggttaagga
      661 aaataaattt agagaagact tatactatag attgaatgta atacctttag aagtgcctcc
      721 acttagagaa agaggagacg atatacaaat attaataaac aattttatag acaaatattc
      781 gagaaagatg aagaaaaacg tgtaccatat agagccagat gtggaacaaa tgttgcttaa
      841 gtatccttgg ccaggaaata ttagagagtt ggaaaatact atagaactgg cattaaacct
      901 tcttgaggaa gatggaataa tacataaagg aataataaat agaaagatac ttgaatattt
      961 taagttaaat aatataaata taaagttact tgaggaaagt gactatgaga taagtatgga
     1021 acaagacata cttacacttg aagagtttga aagagcctat ataaagaaag tgttaaagtt
     1081 ttacggaaat gatactaagt ctaaaaaact ggctgccaaa aaacttggaa tatcactagc
     1141 tacattgtac agaaaaatag atgtataata attaggcttt ttattctcaa ttctaaaatt
     1201 tgaaattata aaattgataa aaaacacttg gaaaactatt ttctaagtgt ttttttgtgt
     1261 aatagcattt tattatccaa aaataaaaaa aatttaaaag aatttaaaaa aataaatgct
     1321 ttaataaatt atcaaatact aacaaatata ataaaaatta tatatgtaaa acaattatgt
     1381 taaaaaattg gcattataat tgcttcatat taagttgaga tgacaaaata aaaaaagaaa
     1441 ggatgttgtg tgagataata ataactgtca ttaaaaatag ttgggagtga taaccttgag
     1501 taaatatcaa aagaatgttt ctatgtttga tattgatgga aggccaccaa ttttaaagtg
     1561 tttgccacta tcaatgcaac atatactagc aatgatagtt ggaactgtta cagtacctat
     1621 agtcgtagga ggagctgtgg gtgttagccc tacacaaata acacttttag tgcaatatgc
     1681 attaataata gctggtatat caacacttat acaactatat ccaataggaa atattggttc
     1741 aagattgcct ataatttttg gagttggttt tacttatgta ccaacacttg tagcagtcgg
     1801 aggaaattat ggattagcaa gcattttggg tgcacaatta atagggggag tggctacagt
     1861 attaataggg atattcataa aaaagataag aaaatttttt ccgaatatag ttgcaggaac
     1921 agtggttttt acaattggat tatctttgta tcctatagca ataaattata tggcaggtgg
     1981 tgtaaatagt cctacttatg cctctatgca aaactgggca gtagcggcta taacattagt
     2041 ggtagttata ggtcttagtc aatttgcaaa aggatatgca aaattggcag caatattttt
     2101 gggaattata gttggatatt gtgtatcttt agttctgggt ataataaatt ttgaccctat
     2161 tagagaggca acttggtttg cgatacctaa accgcttgaa tttgggatgg aatttgatat
     2221 gcctgtaata atctcagtag taattgtaag tataatcaat gctgttcaag caattggaga
     2281 tttatcagct actacaatgg gaggcatgga taggaatatc acagataagg aattatcaag
     2341 tggagtaata ggaagtggaa tttgtactat gataggagct ttgtttggag gtttaccacc
     2401 atcatcatat agccaaaacg tggggatggt agctatgaac aaagtaataa gtagatttgt
     2461 gcttggaata gctggtatat ttatgttatt gtcggggttt gtgcctaaat ttggtgcatt
     2521 aatgactact ataccatatt ctgttttagg aggagctaca atatctatat ttagtatgat
     2581 aacaatgaca ggtataaagt taattattac agaagagttg tcatcaagaa atattaccat
     2641 tgttggtttg gcaattgcct taggcatggg aataacatca gtaccagcag cccaagaggc
     2701 atttccttca tttgtaaagt tagtatttgg agaatctcca atagtaattg caacattggt
     2761 tgcattcgtt ttaaatctaa taataccaaa taaaagtcta gaggatgagg ctaaagaaag
     2821 agctgttatg gaagagacta attaagatat aataatttaa aagaattgtt taaattattt
     2881 agcatgagat tgttagatta tttttgatag ctattcattt taataaaaaa tttaacaaat
     2941 aaatatatac caggtaatta atgttcattt aagttgaaaa tcggggaggg aatatttaga
     3001 aatttgttaa ttttttataa aaatagctca gtttagctgt gtttatcggt tgagcaataa
     3061 attcatggtc ataaaaatat ttatagaaaa taattataaa aatatttagt caagatattt
     3121 atgtatggga ggatttattt tatgaaaaca cgtaatactt caaaatatga tgtcgatggg
     3181 ataccaccac taaaggaatc aatacctttg gccttacaac atctgttagc aatgatagta
     3241 ggaaatatgg tacctgcgat attaatagca aacgtggtag gacttaacca ggggcaagca
     3301 acaatgttga ttcagggttc tatgttagca gcaggtttag caacgttttt acagttatat
     3361 ccaatacctt tatttaaagg atttaagtta ggttcaagat tacctgtaat gatgggaatg
     3421 agttatgtat ttttaggagc ttgtctttca gtagcagcta aagatggttt agcagctcta
     3481 tttggagcac agattgctgc tggggtaatc gtattctttg taggatttgg agttaagaaa
     3541 attagacata tattcactcc aatagtttct ggaactataa tagcatgtat gggacttgga
     3601 ctattcgcaa cagctataaa gaatttagct ggaggagaag gcacacaaac atttggaagc
     3661 ccaataaact tcacagtagg agttatagtt gcttttgtaa taataatgat aaataaatat
     3721 ggtaaaggtt tagtaaaaaa ttcttcaata ttaataggaa tattagtagg ttatgctata
     3781 tctttagttt taggattggt tgatttctca gccgttcaag gagcagctat agtatcttta
     3841 ccaacaccag cagcttttgg attagaattt agaccagagt taatagtaat gtttactatt
     3901 atttacataa taggtatagc tgatatgatg ggtgcctgta caatagctac agtaggagca
     3961 atggatagag aggtaactga cgaagaatta gcatcagttg ttttaggaaa ttctataact
     4021 tctataattt catcactatt tgcagctctt ccaactggag tatttagcca aaatacagta
     4081 attgtatcta tgaacaaagt tacaagtaga tttgtaatag cactaggggc acttgtatta
     4141 ttactagcag ggatatctcc agcattgggt gcaataatga caactatacc atcttgtgtt
     4201 gttggtggag caactttagt agttttctca tcaatagcaa tgtctggttt ctctattatg
     4261 agtatggatg gattcactga agaaaacaat cttatagcag gtgtatctat agcaacaagt
     4321 atgggtctta caactgctcc acaagtttta gaccaattcc cagagacaat tggaactgtc
     4381 cttggagggt caagtatagt ttctggagca ataatagcta tattacttca aacattattt
     4441 aaattaaagt cacgtaaaag tgctgaaaac gttacttcaa atcttgagga aaatattgga
     4501 taaatgtatt agatataata tgagaaaacc atatggtaaa atgttgtaat gaaagtattc
     4561 aaagggggat ttaagatgat tgatatacta ataaaaaatg caataattgt tacagttaat
     4621 aaagacagag atgttatatt tgatggagca atagctattc aaggtgacag aatcttggac
     4681 ataggaaata cagttgatat tgagcctaag tatcttgatg ctaaaaaagt tatagatgct
     4741 aatggaaaag taatattccc tggctttata aatactcata atcacctgtt ccaagtgtta
     4801 ttaaaaggtt taggtgatga tatggcactt catgagtggt taaatacaat gatgtttcca
     4861 tcagcaaaat tcttaacaga acaagatact tatgatgctg caatgcttgg atgtatggaa
     4921 ggtttaaaaa gtggtataac tacaatggtt gattatatgc atactcacaa tagaccagga
     4981 cttacagatg gaatagtaaa ggcatataaa gatttaggta taagaggggt tgtaggtaga
     5041 ggttgtatag atttaggtat tcacaaagag cttatagaag atgtggaaac ggttgaaaaa
     5101 gatttgagaa gggtattcga aaaatatcat aattcagaaa atggaagaat aaaaatctgt
     5161 gtagctcctt cttcaatgtg ggctatatct gaagaaatgg gtaagatgct ttggaatata
     5221 gttaaagaat atgattcata ttttacagtt catatatctg aaacagaatt tgcaagaaca
     5281 gcaacaaagg atatacatgg agaaatagat ataaaattac ttgaaaaatg gggtatagta
     5341 ggtccagaag ttgtagcagt acattgtgta tgtataactg atgaagacat ggaaatgctt
     5401 aaaaagtatg acataaaagt ttctcataat gtagcaagta acatgtatct tgcttctggt
     5461 gttgcacctg tgccagaaat gttaaaaaaa ggtataaatg tgagtttagg tcttgacggt
     5521 gcagcaagta ataacgcaca agatatggta gagcttatga aactaacagc tttacaacac
     5581 aaagtaaata attgtgaccc tctggcaatt tctgcagaga aagttcttga aatggctaca
     5641 attgagggag caagaactct tagaatggaa gatgaaatag gttctttgga gattgggaaa
     5701 aaagctgacc tagtaatatt taactcaatg ctttctccaa aatcaatacc tcttcacaat
     5761 cctgtatcaa ctttggtata ctcagctagt atgcacaatg tagagagtgt tttagttgat
     5821 ggaaatgtaa tacttgaaaa tggaaaagta actacaattg aaagtgaaga acaagtgttg
     5881 cttaatgcac aacatgcagc agaagaatta tgtaagagag ctaatgtaac taatagaatg
     5941 gaaggacata agtggaatag cttatactaa tattaaaata agattgtaat aatattctat
     6001 tacaacatta gtttatctgt aggaggagaa aatggaaaaa ataaaattaa atattaatga
     6061 ttacatgtat gaaatagaag ttgaaaagaa tgaaactctt ttgcatgtac ttcgtgaaaa
     6121 attaaatcta actggagcga aacaaggttg taatagtggc agttgtggag catgtaaagt
     6181 tttgatagat ggagttgaca tgaaatcttg taaacttctt gcaaaaaact gcgaaggtaa
     6241 agttatatac actatagaaa gttttgctaa tggagaaaat cttcatccaa tacaagaagc
     6301 ttttatagaa gttggagctg tacaatgtgg atattgtact cctggtatga ttataacaac
     6361 acaagctctt ttaaatagaa atgcatctcc taatgaagat gaaattagag aagcttttaa
     6421 agaaaatctc tgtagatgca caggatatgt aaagattata gaagctgtaa aattatctgc
     6481 agctagatta gggggtagat aattatggaa aaggtaatag gaaaatcata ccctctaaaa
     6541 gatgcagctt taaaagttac tggtcaaatg aaatacgtgg ctgatatgaa accacaaaat
     6601 gtactttacg ctaagatgct tttaagtcct gtagcacatg caaaaataaa aagtatagat
     6661 acaagtgaag ctgagaaagt agtaggtgtt agagctatag ctacttatct caatacatca
     6721 caaagaaaat acaatggttc attaagattt tatgagcaca aaatacctga aaatgaagtt
     6781 atattttcag atacagttag atttgttggt gaccgtgttg cagcagtagc cgcagagact
     6841 ccagaaatag cagagaaagc tgtaaaactt ataaaagttg aatatgaaga attgccagct
     6901 gtatttacta tacaagatgc aataaaagaa gatgctatct gcatacatgg agattcaaat
     6961 atagtttctc aaaacaacat aaatggggga gatgtagaaa aaggcttcaa ggaatgtgac
     7021 tatatatttg aagatgaata ctctactcca gcaatacatc atagtgcaat agagactcat
     7081 agtgtaattg cacactatga ttataatgat aaattaactg tacatacacc ttgccaaaat
     7141 acatttggat ttagaataat actatctcaa atttttggga tgtcattaaa taaagtaaga
     7201 gttataagac ctgctattgg aggctcattt ggtggaaaat tagaacttac aattgaacca
     7261 gtagcagccg cattatcaaa aatgacaaga agaccagtta aaatggttct tacaagaaaa
     7321 gaatctatga tatcgacaag aactcgtcat gggtcttttg taaaattaaa aactggtgta
     7381 aataaagatg gtgaagtaat agcacaagat ataaaaatat atacaaatac aggagcatat
     7441 gcatcaagtg ctttaaatgt tataggagca cttagtcata aagtatttaa agtttataaa
     7501 ataccaaata taaagtttac tgggatgcct gtatatacaa atacaccaat agcaggagct
     7561 atgagaggct atgggtcacc tcaaatattt atggctcaac aagctcaatt tgcgaaaata
     7621 gcaaaggaaa taggtatgga tttagtcgat tttcaaaata aaaatgcagt agagccagat
     7681 gatgtcgata taatcttcca cggttctctt ggaaatccta gagtattgga ttgtatagag
     7741 caaggtaaga agatgtttaa gtgggatgaa aagaaaaaac ttccaaaaga agaaggaaaa
     7801 tatttaagag gtataggtat ggcaataggt gcacatggta atggagtttt tggtgcacat
     7861 agagatgttg ttacattgac tcttaaatta aatgaagatg gtacgttgac actactaaca
     7921 ggagctcatg atatgggtaa tggtgttgta actatgcaaa caatgatggt agctgaagtt
     7981 ttaggaataa cacctgataa agttgataca tttgaaactg atacggatgc atgtacattc
     8041 aaccttggtg actatgcaag tagaggggta tttgtagaag gtggaggagc taaaaagaca
     8101 gcagagaaat tgaaaaatct tatattagaa gaagcagaaa aattacttga aacatcaaaa
     8161 gaagatttat atctagacaa tggatgtgta attagtaaac ttgatgaaaa tgtaaaagca
     8221 tcactttctg atgtcgcagt atatagtcaa agtaaatcat taagagagtt aactgttgtt
     8281 gaagattatt cttcaccagc tggacgtact tcttatggtg tacattttgc tgaagtttta
     8341 gtagataaag aaactaagga tataaaagta gttgattttg tagctgtaca tgatgttggt
     8401 agagttataa atcctctaaa tttagaagga caattagaag gtgggattca tatgggtcta
     8461 ggatacgcat tgagtgaaga attggcattt gatgaacaag gtagacctaa agctactgat
     8521 tttaaaacat ataaattatt tagaactgta gatatgccaa aatgtcaagt tgcatttgta
     8581 gatggttatg aaaaagctgg gccttttggt ggtaagagta ttggagaatg tgccgttgta
     8641 ccagttgcgc cagctgttgc aaatgcagta tataatgcaa ctgatgtaga gatacattca
     8701 ttaccaataa aattaaaata gtataaatta aagcagaaat aacaatttat tgtatttgta
     8761 aatagataat aaaaatagat ttatttggtg aggagatatt attatggcaa agatattagg
     8821 tatttgtgga agtccacgta aaggagcaac agaatatgca gtgttagaag ctttgaaaga
     8881 agcagaaact atacctggta tagaaactga gtattggtca gttagaggga aaaaaataag
     8941 tccttgtgta cattgtgatg catgtataag aaaagaaact atgtgtatta taaaagatga
     9001 tatccaagaa ttagagcaaa aaatattgga ggcagatgga attatagtag gttctccagt
     9061 ttatgatatg aatataactg ctcaacttac agcagtattt aatagacttc gtccaatgta
     9121 tttagttcat ccaggaaaac ttcaaaataa ggttggtgca gctataacta ctggtggaac
     9181 tagatatggt ggccaagaat tgacaaaact tcctataata aatttcttcc taatgcatga
     9241 aatgttagtg tctggaggtt taggtggatg ctatataggt ggtacaatat ggagtaaaga
     9301 tggaaaagca aaaggtgccc aagaggatga gacaggtatg gacacagtaa aaagacttgg
     9361 aaaaggtgca gcagaggcag ttatggtttc taagtttggt cttgaaaaat ggaatgtcgt
     9421 aaaagaagaa ttagatgtta aaaaagatga aaaatcacct cttagagacc actaaagatt
     9481 tattatatta ttgaatatat acaaatagtg atgtaataga ttattgcatc actattttat
     9541 gttttaattt agattttata gattttatat taaaaaatat agaaacaaag tgttttgaga
     9601 tttattttta aattttaaaa atgagaatta aaaagtgaga agatagaaaa ggttttttag
     9661 ttattaaatt ctacatacat tgtatgaact aaagatagaa aagaaaaaat aataaaagca
     9721 ttgcaatttc tatactaaag agtcatagaa aaatatctag tatatattta gtgttggcat
     9781 gttaattgct tatatattaa agtataaaaa tttaacaata atttggaggt attttagatg
     9841 ttaaaagaaa ttaaatggaa agtaaataat ttgccaaagg gcgataagga aaattgtatc
     9901 aaatttttaa atgaagaaga aataactaag gttagaaatt ttcacaaaag ctttcctcaa
     9961 tataaagaaa ctccattagc aaacctagaa ggtttagcaa aaaaattagg tgttgcagga
    10021 gtatatgtaa aagatgaatc ttatagattt ggattaaatg catttaaagt attaggtggt
    10081 tcttattcta tgggtagata cttagctcaa agattagata cagatatatc tgagttaggg
    10141 tatgacaaat taacttcaaa agaaataaaa gaaaaattag gagaaataac tttctttaca
    10201 gctacagatg gaaatcacgg tagaggagtt gcttggacag ctaataaatt aggtcaaaaa
    10261 tctgttgtat taatgccaaa aggttcttct gaatttagat taaataaaat taaaggagaa
    10321 ggtgcagatg ctagtataac agacttaaac tatgatgatg cagtaagatt agctaatgat
    10381 tatgcagaag ctgatgacca tggagtaatg gttcaagata cagcatggga cggatatgaa
    10441 gaaataccag cgtggataat gcaaggatat ggaactatgg ctcaagaagc tatagagcaa
    10501 ttaaaagaat atggagtaga tagaccaaca cacgtatttg ttcaagcagg agttggttca
    10561 cttgctggtg cagttcaagg gtatgttgct tcaatatatg atgaatgccc aataacagta
    10621 gtagtagagg ctgatgaagc tgattgttac tataaatcag cagaagctgg agatggaaaa
    10681 ccaagatttg ttggaggaga tatgccaact ataatggcag ggcttgcttg tggagagcca
    10741 aatactatag gatttgaagt attaaaaaat catgcagcag catttgtttc agcaccagac
    10801 tgggtttctg ctaagggtat gagaacatta ggtaatcctt taaatggtga tgaaaaggta
    10861 atttctggag aatctggagc agttacaact ggtcttttag tagcagctat ggaaagagaa
    10921 gatttagcag atttaagaaa agatttaaaa ttagatgaaa attcaagaat acttttaata
    10981 agcacagaag gagatactga ccctgacaaa tatagaagta tagtttggga tggagaatat
    11041 ccaagtatat aaggaattac taaaatgcaa ttattagata ttggtttaca tatttagaag
    11101 atatataagt tctaaaattt agaaaatata taagtatata aaatacatac attataaaaa
    11161 atctaggggg ataatgaaat gttaaacgaa caaagaaaac aagaagtaac tgaaatatgc
    11221 caaaaattaa ttcaaaatcc tagttcttct ggaaatgaag aaggtgttgt aaaagcaata
    11281 gaagagtctt ttgaaaaatt aggttttgat agttggtcaa gagatagata tggaaatata
    11341 gtaggatgta taaaaggtaa caagccaggt aagaaaatat tatttgatgg acatatagat
    11401 acagttccag ttccagatgc aagtaaatgg agtgttcctc cttttgaagg aaaaatagtt
    11461 gatggaaaga tatatggtag aggaacttct gacatgaaag gtcaatatac tgcaatgatg
    11521 tctgctgtag cgtattttgc taaagacact aatagagatt ttgctggaga actatatgta
    11581 gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcgg ctagagagat aagtaaggct
    11641 gttcaacctg actatgttgt aataggtgag tcttcagagt taaacttaaa gattggtcag
    11701 agaggtagag gagaaatagt agtagaaact tttggtaaac cagctcactc tgctaatcca
    11761 gaaaaaggt
//
LOCUS       DAELJO010000120        11720 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELJO010000120 DAELJO010000000
VERSION     DAELJO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.120"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            210..674
                     /locus_tag="KPB88_002598"
     CDS             210..674
                     /locus_tag="KPB88_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG0054866.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            681..1175
                     /locus_tag="KPB88_002599"
     CDS             681..1175
                     /locus_tag="KPB88_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG0054867.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1191..2216
                     /locus_tag="KPB88_002600"
     CDS             1191..2216
                     /locus_tag="KPB88_002600"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG0054868.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2203..4650
                     /locus_tag="KPB88_002601"
     CDS             2203..4650
                     /locus_tag="KPB88_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG0054869.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4830..6203
                     /gene="radA"
                     /locus_tag="KPB88_002602"
     CDS             4830..6203
                     /gene="radA"
                     /locus_tag="KPB88_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG0054870.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6206..7276
                     /gene="disA"
                     /locus_tag="KPB88_002603"
     CDS             6206..7276
                     /gene="disA"
                     /locus_tag="KPB88_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG0054871.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7365..8459
                     /locus_tag="KPB88_002604"
     CDS             7365..8459
                     /locus_tag="KPB88_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG0054872.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8588..10765
                     /locus_tag="KPB88_002605"
     CDS             8588..10765
                     /locus_tag="KPB88_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054873.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10951..>11720)
                     /locus_tag="KPB88_002606"
     CDS             complement(10951..>11720)
                     /locus_tag="KPB88_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0054874.1"
                     /translation="SGSYVANHWHNSLEIIYITSGDLQINMEGYTYNLKANECMFINS
                     GIIHSTRCIYQNTSILLQVPLSFLNKYIPDFKNCYFDFKVNANDNNYKKNSSKVKLIL
                     ENMREIKLSSPPAANLLFTSLLFELLFELYTNFKISVGNKNMKKTVLDLSKFEPVLEY
                     TKINYKMPISINKIAKVAHLQPEYFCRKFKQYMGQTYLEYLNDVRISHIYKDLINTND
                     TLCSILEAHGFTNTKLFYRIFKEKFKCTPKHIRKKST"
BASE COUNT         4857 a         1198 c         2111 g         3554 t
ORIGIN      
        1 tctgaaatct aatcatgaag caaaaaatta gcatttggtt atcaataaat ataataaata
       61 aaaactcata attaatgaaa gaataaattc atatctaaat ttctataaaa aatttaaatt
      121 tatacaattt agggctttaa aaatttaaat aataggtgta aaataatagt caaagaaagt
      181 caaaaataaa aaggtgtgtg gtgatattaa tggcaactat gacagatata attgaaaaat
      241 ttattaaaga tttgatggaa gaagataata gcatacaaat tcaaagaaat gaactagcaa
      301 acttgtttag ttgtgcacca tcacaaataa attatgtctt aacaacaaga tttacaatag
      361 atagaggata ttacattgaa agtaaaaaag gtggcggagg atatgttcaa atagaaaaaa
      421 tacgaaaaag taaagatggg catattagag aactactaaa tgaaaaaata ggaagtcaga
      481 tatcctataa aaaagcaaaa gaattattag aaggtttaaa agagtcggat ttgattaacg
      541 aaagagaatt aaaattaata ttatatgcaa tagatgataa atcattgtgt atgccaatat
      601 atgaattaaa agagaaggta aggtctaata ttttaaagaa tataataata ggattattta
      661 gtatagagga gtgataattt atgctatgtc aaaaatgcaa taagaataaa gcatctgtat
      721 attataataa gatagtcaat ggagaaaaaa cagagatgta tttatgtagt gagtgtgcaa
      781 aagagaatac agaaatgaat tttaacttag atatgccttt ttcaatgatg gatatatttt
      841 ctaatttagg ttttcaacct aaaaaagaac ttgaggaaaa gctagtttgt cctaaatgca
      901 atacaacata tagtgagttt aagaataatg ggagatttgg atgtagtgaa tgttataatg
      961 catttagtag tcaagtaaat ccaatgcttc aaaatataca tgggcatata gaacatacag
     1021 gaaaagcacc taaaaaatct ttttataaga taagtgtaga aaatgagatt aaagaactaa
     1081 aagaagattt agatagagct ataaaaaatg aagagtatga attagcagct caatttagag
     1141 ataaaattaa atatttaaaa ggttctattg actaagattg gagtgatatt atgaaagaga
     1201 acatagtcat gaaatcaaga gttaggctag ctagaaattt aaataactat ccatttccaa
     1261 ataaattaga taaagaatgt gccatggaaa taatagaaaa agtaaaaaat gcgtttatta
     1321 attctaactt agaacaaaaa gaagaatttg atttctataa aattgaagat ttagaccaat
     1381 ctaaaaaaat gcttatggtt gaagaacata taataagccc agatttagca gaaaatgata
     1441 aaagtgcagt tatagtaaaa aaagataaaa ctataagtat aatgataaat gaagaagacc
     1501 atataaggat acaaactata tgtgatgatt taaacttaga atatgcatat agtgtagcaa
     1561 atgaaataga tgatttgtta gaatcaagtt tagaatatgc ctttaacaca aagctaggtt
     1621 atctaacatc ttgtccaact aatacaggaa caggaatgag agcttctgta atgatgcatt
     1681 taccagcatt aagtcaacta ggctatatgg atgaacttta taaaatttca tcacaaatag
     1741 gtatagcaat tagagggata tatggagaac gaacagaagc attgggaaat atatatcaaa
     1801 tatctaatca acttacactt ggaagaacag agtctaatat tatagaaaat gtaagtggac
     1861 ttacaaaaga tgcaatttca aaagaaataa aagctagaga aatattgcaa aaaaaattag
     1921 gtataaaact agaagataaa atatttagat cgatagggac acttgaaaat tcaagagtaa
     1981 tgagtagcgc agaggcaatg agccatctat caaatataaa aatgggaata gaaatgaact
     2041 atatagataa actagattta agggctatag aacaacttat gataggtata caaccagcgc
     2101 atcagtcaat aatgtataag agtgatgatg ttgagaatag agatataaat agagcaacat
     2161 atattagaga aactttggaa aaattaagag gagggatgaa ttgtgaactt caatagattt
     2221 acacaaagag ctaaaaaggc aattgattta gcgtttgaat ctgctaaaag tctaggacac
     2281 aatattgtcg gaagtgaaca tatactttta ggtcttttaa gagaagaaga aggtatagct
     2341 gctaaagttc taagtaaagt tggatttaca gaagcttatt tagaaggcaa aatagttgat
     2401 atggaaggta agggagaaga aatctcagaa gatatagtat taagtccaag aagtaaacaa
     2461 atacttgaat tatcaggaat gtttgcaaat aaattaaaaa caaattatat tgggactgag
     2521 catatattat tagcaattat tcaagaaggt gaaggaatag ctaataagat tttaaattat
     2581 gctggagtaa atgataggac tttagcccaa ttaacaattg atatgatggg tattagtgat
     2641 aaaaatcaat acaaagcaga aaatagttac acgggcaacc agaatcaagc agaatctaaa
     2701 gtacttgata agtatggaag aaatcttaca ctatatgcaa aacagaataa aatagaccct
     2761 gtaataggta gagaaaaaga aatacaaaga gtaatacaaa tattaagtag aagaactaaa
     2821 aacaatccag tgttgatagg agacccaggt gtagggaaaa cagccatagc tgaaggtctg
     2881 gctacaaata tagccttagg aaatgttcca gaaacactaa aaaacaaaac tttatactct
     2941 ttagagatgg gttcattatt ggctggggct aagtatagag gagaatttga agaaagaatt
     3001 aaagaagttg tagatgaagt tgttaaaaat ggaaacataa tcttatttat agatgagatg
     3061 cacacaataa taggggctgg gtctactgga gaaggctcta tagatgcttc aaatatatta
     3121 aagccagcct tagcaagagg agaaatacaa gttataggtg caactacaat tgatgaatat
     3181 agaaagcatg ttgaaaaaga ttctgctcta gaaagaagat tccaaccagt tatggtagat
     3241 gaaccaacta aagaggactc aataaaaata ttagaaggat taagagataa atatgaagct
     3301 catcataaag ttaaaataac tgatgatgct ataaaggcag ctgtagaatt atcaactaga
     3361 tatatatcag acagatatct gccagataaa gctatagact tgattgatga agctgcatca
     3421 aaagtaagat taaaagaaaa tacacctcct tcagaaataa aaaaattaga actagaaata
     3481 gaaaatatag ataaagaaaa agaagaagca gtaagatgtc aagactttga gaaagcagcc
     3541 aaaataagag atgaacaagg tctacttaaa aaacaattag aagatgttag agaaagatgg
     3601 aacaagtcat ctaaacattc agatttagtt gatggagaag ttattgctga agtagtgggc
     3661 ttatggacag gaataccagt taataaaatt cttgaagaag aggctgatag acttttgaga
     3721 cttgaggaaa tactgcacaa tagagttata ggtcaagaac aagcagtaaa atctatttca
     3781 aaggcaatca gaaggtcaag agcaggtctt aaagacccta atagaccaat tggttcattt
     3841 ttatttttag gtccaacagg agtaggtaaa acagaattat ctaaggcatt agcagaagtg
     3901 caatttggag atgaaaatca aataattaga atagatatgt ctgaatatat ggaaaaacat
     3961 gctgtgtcaa gaatgatagg ttcacctcca ggttatgtag gtcatgatga aggtggtcaa
     4021 ttaactgaaa aagtaagaag aaatccatat tcagttatct tatttgatga gatagaaaaa
     4081 gctcatccag atgtatttaa tattctgtta caaattctag atgatggtag actaactgat
     4141 tcaaaaggaa gaactgtaga ttttaagaat actattgtaa taatgacatc aaatgttgga
     4201 gcatctacaa ttggtagaca aaaaacttta ggatttagta tagctaaagg agacgaagaa
     4261 gaaaaatctc aatatgaaaa aatgaaagaa aatataatgg gtgaattaaa acaaagattc
     4321 agaccagagt ttttaaacag aatagatgat ataattgttt tccactcact aaatgaaaat
     4381 catatatcaa aaatagtact tttaatggca gcgaaactac aagagagatt aaaagaaatg
     4441 gatataaaat tagaaatgag tgatgaggct gttaaattaa tttctaaatc tggatttgac
     4501 ttagaatatg gagcaagacc tcttaaaaga gctttacaaa aagaattgga agatgagcta
     4561 tctgaagcaa tcttaaaagg taatgtgaaa aagggaagta atgtagtagc aaaagtcaaa
     4621 gatgaaaaaa tagtttttga aactaaataa tttaatagag ttgcatttta aaatattgca
     4681 aatataagtt ttataatcta aacaaaattt taagggcaat gaatctataa taattcattg
     4741 tccttaatta ttttatttaa tctttacata ttgtctttca gatatgctaa aattatagta
     4801 tagaataaaa aattaaggat gataaaagta tggcaaaaat aaaaacaaaa tatgtatgtc
     4861 aatcttgcgg atatgaaaca gctaaatggc ttggtaaatg tccagaatgt acaaaatgga
     4921 atacatttgt agaagaaata gaccaaaaaa gcaccaaaaa agaggtcttt ataatagata
     4981 aatcttcatc taaaccagtc agtataaact caatagaaag taaagaagaa gagcggttta
     5041 caacagatat aaatgagtta gatagagtgc ttggaggagg tatagttaaa ggctctttag
     5101 tgcttgttgg tggtgaccct ggtataggta aatcaacact tttaatccaa gtatctagta
     5161 atgttgccaa tttaggaaaa actgttttat atataactgg agaagaatca gaatctcaaa
     5221 taaaaatgag agctaaaaga ttaggtatta actcagaaaa tttatatata tttgctgaaa
     5281 ataatttaag tataattgaa tcttatttag aaagtgtaaa tccagaatta ataattctag
     5341 actcaattca aactgtcttc agtccagaaa tatcctcggc accaggaact gtcagccaaa
     5401 ttaaagaagg tacttcaaaa ttcatgaaaa tttctaaaaa aatgggaatt tcaacgttca
     5461 tagttgggca tgttacaaaa gaaggttcac tagcaggacc aaaattatta gagcatatgg
     5521 ttgatactgt tctatatttt gaaggagaaa gatataatac ttatagatta gtaagagctg
     5581 ttaaaaatcg atttggttca actaatgaac ttggagtatt tgaaatgagg gatttaggat
     5641 tagtagaact tgataatcca tctaaaattt taatctctga aaaaccaaaa gatgtagcag
     5701 gttcagtaat aatatctact gtagaaggta cgagaccaat gttacttgaa ttgcaagcat
     5761 tagtttctcc tacaagtttt ggtataccaa aaagaacatc tacaggagtc gattataata
     5821 gagtaggtat gttattagca gtattagaaa aacgtgttgg tttgcaaata caaaatcaag
     5881 atgtctatat aaacatagtt ggaggaataa agataaatga accctcaatt gatttaggga
     5941 tagccatttc tgtagcatct agttttagaa atataccgat agatgaagat atagctgtta
     6001 caggagaagt gggacttaca ggagaagtta gagcagtaag ctttatagaa aagagaatag
     6061 ctgagtgtaa aaaacttgga tttaaaaaga tagtagttcc tagaagtaat tatgatgttg
     6121 taaaagagac caaaggcata gaaatatggc cagtagataa cttaagacaa gctataaata
     6181 tagtacttgg gaggaatcaa taaatatgga gaattttcta gataataaaa atatgctata
     6241 tgcattaaaa atgatatctc ctggaactcc acttagatta ggtctaaaca atgtactaag
     6301 agctaagact ggtggattaa ttgtaattgc aacaaacgaa gatgtaatga aaatagtaga
     6361 tggaggattt gctataaatg cagaatattc accatcatat ctatatgaat tagctaaaat
     6421 ggatggagct atagttttaa gtggtgatgt aaagaaaata ttatttgcta atgcacaact
     6481 tatacctgac tattttatag aaacatcaga gacaggaaca agacatagaa cagcagaaag
     6541 agtagcaaaa caaactggtg ctatagtcat aggaatttca caaagaagaa atgttataac
     6601 agtttataga ggaaatgaga agtatgtagt cgaagatata tctaagatat ttactaaggc
     6661 aaatcaggct atacaaactc tggaaaaata taagacagta ttggaccaag ctgtaacaaa
     6721 tttaaatgcc ttagagttta atgatttggt aactatttat gatgttgcat tagtcatgca
     6781 aaagatggaa atggtaatga gagttacaag tataattgaa aaatatgtga tagaattggg
     6841 tgatgaagga actttagtaa gtatgcaatt agaagaatta atgggtacaa ccagaataga
     6901 ccagaaatta atattcaaag attataataa agaaaacaca gaaataaaag aacttatgaa
     6961 aaaggtcaaa aatttaaatt cagaagaact aatagaattg gtcaatatgg caaaactatt
     7021 agggtatagt ggtttttcag aaagtatgga tatgcctata aaaacaagag gttataggat
     7081 tcttagcaaa atacatagac taccaacagc aataatagaa aacttagtaa attattttga
     7141 aaactttcaa caaattttag atgcatctat tgaagaatta gatgaggttg aaggaatagg
     7201 tgaaataaga gcaacatata taaaaaatgg actcataaaa atgaaacaat tagtcttatt
     7261 agatagacac atatgatgaa ttttaaatga attttactac ttttttcttg aatataatat
     7321 acactttggt ctataataga taagtataac tagggggtga agcattgata aggaaagtaa
     7381 caagaatatt gtttatttta cttggattta caataggtat aacaacctat ttaacattaa
     7441 tgaaagattt tgaaatatta acatttggaa aagaaacata tggttatatc gcagctgtag
     7501 tagcaggtat aataatagcc attttaggat atcttataga accatgggtt gtaaataaag
     7561 taaaagaaat tgctaaaata gtggataaag aattgtcaaa atatcctcag actgatatat
     7621 tgttagggtc tatgggtctt atagtaggat ttgttattgc gtatttatta agtggattag
     7681 taaatagaat accaatagta ggtggaatac tatccttact attatattta ttcctaggat
     7741 atcttggaat gaaagttgcg cttaaaagta aaaatgattt atttaatatt ggaaaactag
     7801 gaagattagc aaatcctatt aaggataaag ataaggataa agaaaataaa aaagaagtaa
     7861 aagccattcc tccaaaagta ttagatacaa gtgtaataat tgatggaaga atagctgata
     7921 tttgtaagac tgggtttata gaaggaaaac ttattattcc tgcttttgtt ttggaggaac
     7981 taagacatat tgcagattct tcagatgatt taaaaagagt aagaggtaga agaggtctgg
     8041 atatattaaa tataattcag aaagaattaa atatagaagt tgaaataagt gagagagact
     8101 ttgatgatat agcagaagtt gatagtaaat tattaaagct tgctcaggtt ttaaatggaa
     8161 aagttgttac aaatgattat aacttaaata aagttgctca attccaaggt gttgaagtat
     8221 taaatataaa tgagttagct aatgctataa aaccagttgc tataccaggt gaagatatgg
     8281 ttgttcaagt ggttaaagaa ggaaaagagg ctcaacaagg tgttgcatat ctagatgatg
     8341 gaactatgat agttgttgat ggaggaagaa aatatatgaa tgagactata aaagtactcg
     8401 ttacttctgt tcttcaaact cctgcaggta gaatgatatt tgcaaaacct aaaaattaat
     8461 atgttcaaag aaacctataa tttgggctgt ttattttaaa tagatggtcc aaatttattt
     8521 atttataacg ataatgttta atgtattctt aaagatgggg atttaaatca gaaagcaggg
     8581 agaatttatg agaaaaaaga ttatatcttt cttagcaaca tttatcataa tattaacatc
     8641 agcaagtcag tatagttttg cagatgatat atcaacacgt ggaaaagtaa tatttataga
     8701 tatgaataga actagtatga gtaatatgtt aagaataaaa tcattaagag aagaactaga
     8761 caatagaggg tatattggct tgatgaatat aagaggcgat aaaggaagtg atgatagaag
     8821 gtcatatgct agtatgggtg ctggtggtag agctaatgtg gctaatgaag aagatattaa
     8881 ttttgaaagc tcgagtaagg atagaaatat agtttttgaa tcagctacag gaaaaagtgc
     8941 aaaaggtata aataatttaa ctataaataa gtctataaat gaaaatttaa actttggaga
     9001 gtatggttct gtattagggt cattaggaca aagtttatct gataatggat taaaagcatc
     9061 tgtattgggt aattcagata taattgaaaa tggtcaactt ataaaaaata gaaacttatg
     9121 tctaactgca atggatgaat atggaagaat acctaatgga aatgtagata ctataaataa
     9181 aaaagactta tctatgcctt atggaatatc aactgattat gataaattga ttgtagagac
     9241 taaagaagct tacaaaaata atgatgtgat ttttgtggag cttggagaca catatagatt
     9301 agatttatat aaaccaaatt taaatgaaaa aacatatgag agtatgaaag ataacataga
     9361 aaaaaatata gatatatatt taaaaaatat attttctatg gttgaagaaa atgacactgt
     9421 ctacatagca agtgcatttc caagtgattt agattataaa aataaaagaa gactttcgcc
     9481 aataataaaa ttaaatggag aagggaaagg tatattatca tcttcaacta ctagaagaga
     9541 aggtattgta acaaatttag atgttggggt tgaaatatta gacaacttta atataaaaaa
     9601 tcaaaatatg gttggaagaa aatatgaact tattaacaga gatgataata aagaattttt
     9661 gatggatgaa tatcaaaaga tagtatctat ttcaagcata aggtctacaa tactgaatgg
     9721 ttttgtaagt atagttttct tgtcatggat agttgctatg attgcaattt tatttagaaa
     9781 gcatatatca aaaaattata aagaaacaac attttttata ttaaaggaac ttttaaaaat
     9841 aggaatagta atgccactgt catttatgat aacaccaata atgaatttca aaacaccatt
     9901 agcaatatct ttaggaataa ttataataac acttactata tatcttataa gtaaagtttt
     9961 aataaagaac gatttaaaaa acatgctgtt ttttactgga ttaaccatag taattatggt
    10021 tattgatgct gggtttggct catatttaat gaaaagcaat gttatgagtt atgattgtat
    10081 aattggagct agatattatg gagtaggaaa tgagtatcaa ggtgtggcaa tcggtagtgc
    10141 aatatttaca tttgcaattt tacttacata taaaaatata ccaaagtggt ctataatagt
    10201 tttttctttg gttatcttaa ttacaagtgc atctccaatt atgggagcaa atgttggaag
    10261 tgctatttct gaatgtgttg catttttact atttatacta ttgatatata atgtaaagat
    10321 agatttcaaa aaaatcgtat tattaggtat agcagtagta tttgtattag gagtttttgt
    10381 agcaatagat atgattttgg gaagtaattc acatttaggc atgtttgtaa aagaaattta
    10441 ctttaatggg ccaggagaaa taatacaaac atttagtaga aagatagaga tgaatttaaa
    10501 gctagctcaa accagtgcat gggtaaatat actgttaact ggaataggtg taattttagt
    10561 tcttatgatt aatcagatta gatactttaa gcaactgatg gatgagtatc caattgtatt
    10621 taaaggattt atagcatcaa tagcaggatg tttagtaact cttttagtga atgactctgg
    10681 gattgtgtca tcagctacag cctttattta tgtaattgta cctatgataa cattaagtat
    10741 aaatcttaca gcactaaaag aataagtact gtatgaataa tatgtaaata ttaatacatt
    10801 aatttaactt ctttatttac aaaataaaga agaaagttta taaaaaataa attataataa
    10861 aaaagagttt atctcaaaat tgattattct aatcaaattt taagataaac tttttttatt
    10921 ataaagataa ataacatata acctcatatt ttaggtagat ttttttctta tatgtttagg
    10981 agtgcattta aatttttcct taaaaattct gtaaaataat tttgtattag taaatccatg
    11041 agcttctaat atactgcata aggtatcatt tgtgttaatt aaatctttat aaatatgaga
    11101 aatacgcaca tcatttaaat actctaagta agtttgtccc atatactgtt taaattttct
    11161 acaaaaatat tctggttgta agtgtgctac cttagcgatt ttgttaatag aaataggcat
    11221 tttgtaatta atctttgtat attctaaaac tggttcaaat tttgacaaat caagtactgt
    11281 ttttttcata tttttattac ctacagatat tttaaagttt gtatataatt caaataaaag
    11341 ttcaaataat aagcttgtaa agagcagatt agctgcagga ggagaactaa gtttaatttc
    11401 tctcatgttt tctaatataa gtttaacttt acttgaattt tttttatagt tattgtcatt
    11461 ggcattaacc ttaaagtcaa aatagcaatt tttaaaatca gggatatatt tgtttagaaa
    11521 agacaatggt acttgcaata atatagaggt attttgataa atacatctag ttgagtgaat
    11581 aattccagaa ttaatgaaca tacactcatt agcttttaaa ttataagtgt aaccctccat
    11641 gttaatttgt aaatcaccag aagttatata aattatttct aatgagttat gccaatgatt
    11701 tgctacataa ctaccactat
//
LOCUS       DAELJO010000121        11688 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELJO010000121 DAELJO010000000
VERSION     DAELJO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.121"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            132..785
                     /locus_tag="KPB88_002607"
     CDS             132..785
                     /locus_tag="KPB88_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0054875.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            926..2197
                     /gene="brnQ"
                     /locus_tag="KPB88_002608"
     CDS             926..2197
                     /gene="brnQ"
                     /locus_tag="KPB88_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0054876.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2399..3586
                     /locus_tag="KPB88_002609"
     CDS             2399..3586
                     /locus_tag="KPB88_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG0054877.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3816..5840)
                     /locus_tag="KPB88_002610"
     CDS             complement(3816..5840)
                     /locus_tag="KPB88_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0054878.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYNKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            6230..6898
                     /locus_tag="KPB88_002611"
     CDS             6230..6898
                     /locus_tag="KPB88_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054879.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            6900..7385
                     /locus_tag="KPB88_002612"
     CDS             6900..7385
                     /locus_tag="KPB88_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0054880.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7446..8567
                     /locus_tag="KPB88_002613"
     CDS             7446..8567
                     /locus_tag="KPB88_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG0054881.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            8848..10038
                     /locus_tag="KPB88_002614"
     CDS             8848..10038
                     /locus_tag="KPB88_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0054882.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    10172..10427
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10486..11487
                     /locus_tag="KPB88_002615"
     CDS             10486..11487
                     /locus_tag="KPB88_002615"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG0054883.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
BASE COUNT         4339 a         1286 c         2024 g         4039 t
ORIGIN      
        1 tgatataatt ataaaagtac tgaatacatg gatatttcgt aaaaactttt taatatatca
       61 aatatgtata ttaagttaat atattaaaaa aaataatatc aaaaataata taatttatag
      121 tggaggggaa aatgactaaa attattaaag tgcaaaaacc taaatttaca ggagaattag
      181 aaattattga aaatcttgaa gatatacttg aagatatatt taatgatgaa aaaatattta
      241 ataaaattat agaaaatgaa gttataagtg atttagattc tgtaagagtt agctttgaat
      301 cttgtatatt tagaaatgtg tcttttgaaa attgtaattt aaaaaagatt gatttacttg
      361 atgtggtatt tgaaaactgc aatttatcaa attcattttt tgatggagga agtatataca
      421 gagttgaatt taaaaatagt aaattgatag gagttagatt tgatgattgt atacttaaaa
      481 acgttttgtt caaggatgtt ttaggtaagt attctaactt ttcattctct aaatttaaga
      541 tagtttctat cttagattca aattttgaaa attctgtatt tcaagaagta aaaggtgaca
      601 atctagtatt tgagtataca aatttaaaaa aagcaaactt taatggaact aaactttctg
      661 gcatagattt tactactaat aatattgagg gaattgagat tggtatagat gatattagag
      721 gagcaatttt tgatgtgtca caagcaattg atttaacgaa attgatggga attatcataa
      781 aatgaaaaaa atattcataa aacaaattgg gaaaacgtta acaatgtgtg aatttttacc
      841 agattgacat ttttctgaaa ataaagtaag attatcttaa tattcaaaat attttcaaag
      901 ggggtaattt agtgtgagta agcagatgag agatattttt atagttggtt ttgctttatt
      961 ttctatgttc tttggtgcag gaaatcttat gtttcctcca tttttaggga tggaatccgg
     1021 caaagactgg ttaattcctc ttattggatt tgttgttgca gatgctggta tagcaatgct
     1081 tgttattata gcaacagcaa gatgcaaggg aagcatggat gatgtactta ggagagctgg
     1141 taaagggtta gcaagagtaa tgagtgtagc tgctctagca tgtttagcac ttcttgtaat
     1201 accaagaact tgcgcaacaa catatgaaat gggtattatg cctatatttg gagaccatgg
     1261 tccagtagct aaagctgtat tttcaataat cttctttggg ttaactttag catttactat
     1321 taggtcttct aaggttatag acataatagg taagtattta actccagcgt tattattagt
     1381 attatttgtt ttaatagtaa agggtataat ttctccagca ggacctatga gtcctgagca
     1441 tatgatagat aaaaatctat ttggtgaagg tatatcacaa ggttatcaaa caatggacgc
     1501 tctaggtgca gcatctatgg caactataat gttgatgtct ataatagcta aaggatatac
     1561 atcagataaa gaccaaatag gaatgactat taaagctggt tttgtagcct gtgtatttct
     1621 agcagtagta tatggtggtc ttacttattt aggtgcaaca gtttcaactt tatatgatac
     1681 tagtatacct caagcatctt tattagttga aataactagt atattgttat atggtcaagt
     1741 aggaaaggta attttaggtg taattgtggc acttgcctgt ctgactactt ctacaggttt
     1801 aacagcatca atatcaactt attttgaagg aataagtaag gcgagttata aacaactggt
     1861 tataggtata tgtgtagcta gtatgcttat atcaaattta ggtgttgatt ctataatagc
     1921 catatcagtt ccaatcctac aaactatata tcctgtttta ttagcaatag ttgttatgga
     1981 gttggctggt aaacatataa agaatgataa tgcttttaaa ggtgcagcat atgttacatt
     2041 agtgattagt ttattatcag ctataaatgg aatgacagga gctgttccat ttatacaaag
     2101 cttgccactt gcaggtttgg gctttaactg ggtaatacca gctatattgg gtggaatagt
     2161 tggtaacttt ataaaaagca acaaacaagt agcataataa tttgtaatta atttaatttt
     2221 ataatatatt aatttttagt actatgggta ggtttaatct aaacctaatc tacccatagt
     2281 actttttaga ttggattata atagcatgta tataattttt tataatataa tttttctttg
     2341 aagtatgata aaattagtaa tagtgataag aatgtacaaa tttaggaggt tttaattaat
     2401 gcttagaaaa ataacagcat ttatgctatc catatgttta attattggac ttacaggttg
     2461 tagtttactt gtaggtaaga aagataaaga gaaaattata gatgatttta aagttgcagt
     2521 ggtaactcaa ccattatctg aaaataaagt acaatacaat atggtggaag agatggcaaa
     2581 agaatatgag gaagagaata aaattgataa agacaaagat ggtcaaacta aagttaaaca
     2641 aactataaag catgttgtat tgccagagaa ttttacttct aatatagata gtgctattaa
     2701 taagatagta aagttggctg acgataaaga agttcaggct attgtagttt caactgacca
     2761 agcagggctt ttacctgctt tacaaaaggt aaaggaaaag cgtccagaaa taataactat
     2821 ttctgcccct atgggagatg ataaaaacca attaagccaa tttgttgatg tgaatctagg
     2881 ggttagtgca gaggaaagag gaaaagtatt agctgaacgt tcaaaagaaa tgggagcaaa
     2941 agcttttatt cattatgcct ctacagatga tttgaaagat gttaatatag ccaaaagatt
     3001 agaaatgatt aaagagactt gcaaaaatat aggattgcca tttgtacaag ttaatactcc
     3061 aaatataaat actgaagaag acaaaaacaa agttaagcag tttttaaatg aagatattga
     3121 aaaacaagtt aaaaaatatg gaaaagatat aaatgtattt ggtgtaaatg agtatatgga
     3181 tgaagttata ttgactaagg ctttagaatt aaagtatata gttgcagagc aaagcaatcc
     3241 atcaccaata caaacatatc ctagtgttat gggtctaaaa atatctgaaa aagatgctca
     3301 aaattatgat aaaataaacg acatgataag tgaaaaggct aaggcttttg gtatgtcaaa
     3361 tagacttggt ggatatccta tgccaatgga tgcttttcta ccatcattag caatttatct
     3421 tgctactgaa atggtgaaac aagatttaac acaagaagat gtatgtgacc cagattattt
     3481 agaggcgttt acagaattaa gatttggaat cggttcagaa tttactcctt taacagaggt
     3541 actttataat tatcaatcag taattttaag ccaattaatt tattaatatt aaggaagtgt
     3601 ctcaaaatga actttttagt ttatgaggca ctctttttct attaaatcaa atatattttt
     3661 atctttttaa caattaaaaa gaagcttatc cctatgttga gatagcttcg tttttaatat
     3721 aaataagttt tattatttta taaaaaattt atagaaaaat aaaaaattat aattatatat
     3781 aaattttgat aaagtaaaga tgaattacta taggtttaaa ttatattatt ttctattatt
     3841 tttatgcctt tctcagttat tgatgagcct tttcttcctt tactagaaat taagtatcca
     3901 ttacgtgtca aatattctag catatttctt acttcgtatt ctgtgacatt atattccata
     3961 aatttcatta actttgtcct ccccattggt ttcatgtgca atgattttag gatagaaatg
     4021 catatgtttt tgttatattg gtttaaatca ttgaatatgg acatattact tgacatatga
     4081 ttattattta attttgaaga tatgttttga ggtaaatcat ttattgtaag ataattggaa
     4141 cccatgtaag ccatatattc aacacaattt ctcagttctc taatattccc catccaagtg
     4201 tatgaattta aagcttctaa aagactctta tcgagtttta tgtttttata gggaagttct
     4261 tttaaaaatg attctaaaag aagaggtata tcttctaatc tctttctaag aggaggtaaa
     4321 tctaaagtga acatacttat cctataaaat aaatccatcc taaatttatt ttctataatt
     4381 aatttctcta aatctttatt tgtggcagct aatattctca catcaattgg aataatactt
     4441 gtaccaccaa tttttctaat ttctttttct tgtaagactc ttaaaagttt aacctgtaaa
     4501 tcatgtggca tatctcctat ctcatctaaa aatatagtgc cagtgtgagc taattcgaat
     4561 aatccttttt ttccaccttt ttttgctcca gtaaaagaac catcttcata tccaaatagt
     4621 tcactttcaa gtaattcact tggtaatgaa gcacaattta ttgctacaaa aggtttattt
     4681 tttctatttg aatgattgtg tattgactga gtaaaggctt ctttacctgt tcctgtttca
     4741 cctgttatta atataggatt atctgtaaga gccatttttt ttgccttttt aatacaatct
     4801 tttataatag aactatttcc aataatatta ttaaatttat atttagctac ataacctctt
     4861 ttgacagaat cacttcttat tttttgttct aatatttcta tattgtttac atcttttaat
     4921 gtaattaagc aaccctctat atttttataa agataaaatg gttttttaga aagtatgaat
     4981 tttttatcta tttcgtctat gtatataatc atattatcta tatttaagtt ctttgtaatt
     5041 ttatctaaga aactctttgg catatattcc attaaggaag gtgattttat aagtttttca
     5101 tctagttcca acatattaag taaagactta tttacaaaag taaccttgtt aaaggtgttg
     5161 aaaaatatta gaccatcacc tatttcatca atggtcatgt aaagtatagt ttttaattta
     5221 gatatattat ctaatgatgt atttaagaaa tcatgactta gtgtctcctt ggacaatttg
     5281 tatagttttt ctataaattt ctcatttttt aattttaata ctactaataa tgacatatat
     5341 gtatctaatg agatttttct ccaacctaaa tcaacaatat tttttatgta ttttggtata
     5401 aatctaatct gacctggtgt tatagctgta tctaaatcat atatttcatc acaatctgga
     5461 tatgtcggta caaaatctat atgatttata ccaattttat atagataagt agccatctca
     5521 aatgctgaag ttttattggt tgtcataagt aacgattttg tacctggagg tagtttcatc
     5581 attttttcat atccaagttt tgaaagtgct ttcgttccat gaataatttc aatatcttcc
     5641 ttaaaatgtt ttattaaaaa attactagtt aaaggtgata gagaaagcac tacatctccc
     5701 tctaaatttt cttttattcc atcaagtata ttttgtgttt ctatgattat ttcgtctcca
     5761 aaaaaatttg ttaattcttc tttgtagtgg tcactattca atttatcata tgttataagg
     5821 attaacttct ttttatccat tttatttctc ctaacaaaat agtatttatt tttaaagtat
     5881 ttaaagtata aggcaagtta tattatacaa caaatagtag tataaattta tatatttgat
     5941 taaataatag gtttttttag gattttatcc tataaaaacc tattttaaaa ttatttttgc
     6001 aagataagga aaatagcttt ttatattgta atgtatattt taaataatta ataaaaaaat
     6061 attatatttt taaacttgaa aagtaagtaa ttataagcta tttaatcgct ggaataaatt
     6121 tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa gttttgctta
     6181 ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata tgagtaaatt
     6241 agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat taatattggg
     6301 agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag tattggattt
     6361 taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg taggtacatt
     6421 agctgcaaac tttagaggtg atggaggtac tggccctcga gatggatggc tatatgcatt
     6481 gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag accacttaga
     6541 tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat tagggttacc
     6601 tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag ctgcatctat
     6661 gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg tattttgtgc
     6721 atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag cagcactatt
     6781 tccatttata ggagatgtac caatatttat accacttgct ttaatattaa ttatgaagtt
     6841 tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg aagcttaaca
     6901 tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata gatgcgttta
     6961 ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca aatgtcatct
     7021 tcggttttgc tataatttct gtgttcaatt taacaggtct tttagatgta atagggacaa
     7081 tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca gctataatga
     7141 caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt acagcaggtc
     7201 aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca ggagcgcaga
     7261 tacaatttat ggggagactt ttaggaacag ctgagttaaa aactaaatat tatccacata
     7321 tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat tatttcgtag
     7381 tataggtaat tataataaaa atttaatata aatggaaagt ttaaatttta aggaggagct
     7441 agattatggg aactaacaaa gattatattg agtatgtaca taaagtgtat gattatctac
     7501 acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt ttaaaagagc
     7561 ttgaaaaaat ggggtttgaa aatattaata aaggagttgc aggaactggt attatagtaa
     7621 ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg gatgcattgg
     7681 aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat ggtcatactt
     7741 ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa agaggtaagt
     7801 taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga gtaattgatt
     7861 ctggtttgct ggatgatgta gaagaaatgg tagggatgca tttaagacct ggacaagatt
     7921 gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata atggaattta
     7981 cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat gcagtagatg
     8041 ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct ataccaaatt
     8101 ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata ccagatgaag
     8161 catttatggc tttagatata agggcagaaa gtaatgatat aatagaagaa ttaaaagaaa
     8221 aagcaataca tgctgtaaaa actgttgtaa gttctataaa tgcagaagct gtactaattt
     8281 ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg gctgaagaaa
     8341 ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat tctggtggag
     8401 aagacttcca ttattataca caaaaactaa aatgtaaaac tacatatatt ggattaggag
     8461 ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa gctttagaat
     8521 atggtgtaga tatatggtgt agattggttg aaaagagatt gggataacat ccaaactaat
     8581 aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt atgttaatta
     8641 gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt ggaatattga
     8701 aaaaatatga tggaaaagta attatatttt aaatctattg gacaaaaaag tgatattatt
     8761 ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatagact taacaattat
     8821 aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag agtaatagat
     8881 agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac aaaagcacaa
     8941 aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga accaataata
     9001 gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa tgatagtgat
     9061 aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga aataaataga
     9121 aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt aagaatatta
     9181 actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt tgaagctaag
     9241 ataaagagca ataataggga cgttgtaaac aatcctctga tatatgagaa tggtacttat
     9301 aggatggatt atgatgattt agaagaaaaa gctaagtgta gcaacaataa agtactaata
     9361 ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt aaaaaaggtt
     9421 gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca ttctgattta
     9481 attagaaaag gatttaaaca tactccttta cagtcattat gcccagagta caaggataaa
     9541 atagttacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca gttgtctaat
     9601 ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac agctgttgga
     9661 gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata taatgaaagt
     9721 gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt tattgatgaa
     9781 tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac atatctagca
     9841 tggttagatt ttactgctta tggattaaat gaagtagaac ttgaagattt aatgtttaaa
     9901 aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat aggatttgaa
     9961 agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag attaaagact
    10021 atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa ataaaaaaat
    10081 aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa atagaaaaca
    10141 taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa gagtagttat
    10201 agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct atagtgaaga
    10261 acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa ccaaaccgtt
    10321 atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg aatttatttc
    10381 aaactaatca gggtggtacc gcggaaaagc aacctttcgt cccttttata tagggatgaa
    10441 gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag cacaataata
    10501 ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat aaaaaaatta
    10561 aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt ttcttctccg
    10621 ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa ggctgtaagc
    10681 tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc aaaatggaaa
    10741 agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac agatatgaat
    10801 gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga ccaatgggat
    10861 tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga tgttgtaaaa
    10921 agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta tgaaggtata
    10981 aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga agatatgtat
    11041 ccagaattat caccaaagga aagagaagat aaaattgtaa aagagaagaa agcagtattt
    11101 ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag aagtcctgat
    11161 tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt agattcagca
    11221 ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag acaattgaaa
    11281 cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt aaatggcgaa
    11341 cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta tttcttacaa
    11401 aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat tcaaaattgt
    11461 agtagtgcag gaattgaatt attataattt aaatataaac taattgataa aatttatatc
    11521 taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat aattataaga
    11581 ggtgataaat atggagtttt taaaaataat cattaatata gttttagata ttttaaaaaa
    11641 aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatt
//
LOCUS       DAELJO010000122        11668 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELJO010000122 DAELJO010000000
VERSION     DAELJO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.122"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..1353)
                     /locus_tag="KPB88_002616"
     CDS             complement(<1..1353)
                     /locus_tag="KPB88_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBG0054884.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSDIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWDVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     E"
     gene            complement(2626..3705)
                     /locus_tag="KPB88_002617"
     CDS             complement(2626..3705)
                     /locus_tag="KPB88_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0054885.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPATVLQTMSAERVLQDVHEVTAYLKQQFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMMDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(3789..4445)
                     /locus_tag="KPB88_002618"
     CDS             complement(3789..4445)
                     /locus_tag="KPB88_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054886.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            4767..4973
                     /locus_tag="KPB88_002619"
     CDS             4767..4973
                     /locus_tag="KPB88_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054887.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(5126..5554)
                     /locus_tag="KPB88_002620"
     CDS             complement(5126..5554)
                     /locus_tag="KPB88_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0054888.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEERYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKMLAE"
     gene            complement(6030..6968)
                     /locus_tag="KPB88_002621"
     CDS             complement(6030..6968)
                     /locus_tag="KPB88_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0054889.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(7482..8294)
                     /locus_tag="KPB88_002622"
     CDS             complement(7482..8294)
                     /locus_tag="KPB88_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0054890.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIIKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(8317..8949)
                     /locus_tag="KPB88_002623"
     CDS             complement(8317..8949)
                     /locus_tag="KPB88_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054891.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            complement(9293..10948)
                     /gene="ilvD"
                     /locus_tag="KPB88_002624"
     CDS             complement(9293..10948)
                     /gene="ilvD"
                     /locus_tag="KPB88_002624"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG0054892.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELETLAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(11272..11571)
                     /locus_tag="KPB88_002625"
     CDS             complement(11272..11571)
                     /locus_tag="KPB88_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054893.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         3686 a         2153 c         1309 g         4520 t
ORIGIN      
        1 ttcgtttata ttttcaatta gatttccttt atcttttctc caaaatgctc tattcttatc
       61 aatacactct ttggaaaatt caaacaatac atcccataaa tcttcatcac tgtttacaag
      121 tgatattttt ttattatatt tatttcttaa ataagatata agttttaaat cctctaaatc
      181 tctaagcact gttctagaca atacaaatat catttcctga atagtatctc taacatcatt
      241 atatcttaat ttgtttgtat ctattagatt ataaacacct tctaaattca tatttttaat
      301 ttctttttca atataaagtt ctaattcaaa gggatattct tttgaagaat tattgaacat
      361 atctatcgga ttaataatat actcttcttc taataatcta ctatttgctt ttttaagtgc
      421 ctcttcaagt cttatatttt cttttctact cttcaatatc tcctcatctt ttatactaag
      481 ttgattttca aaaaaattcc tcttagctat ttcctgaata aattcagaca tcattaataa
      541 attttctttt aatacatata atctactttt aggatttaat ggaaattcat tgtaaatttt
      601 gtttatgtat tttatatcaa tatcagaact acttgataat tcagataaat ttttatctat
      661 atcaatagac ttatatacta taaaattacc acattctata tatccaacta tattttcttc
      721 taatactata ggtatctcta aactggaaac accatactca caatggtaca ctttttcaaa
      781 gttatcatta tttatgcttt taacaagttt tttagtacac tgttttatat ttttacaaat
      841 atcgcaaaat ttattttctt tcttagtctc aaatatttct ttatacttta agtcatatac
      901 agatattcta agcttcaata tattagatat ttgatttgca agattttcaa ttatgtttac
      961 attaactaac tcctgtatat tgttttcaga aaatactata ggtatttgtc tcaaactcga
     1021 aaatttatga ggaacataag taattaatat tactaaatca attttaccag tgtttataat
     1081 ttcatgttct gaatatggtg ggcaataaac tatagatgtt tcctttatat tgaccttttt
     1141 accatcaatt atttgtattc cttctccctg aatcacatat aaaagctgtt cttcacttaa
     1201 atgaaaatgc ttttcttggc agtttcccgg aaaaaatctt acaattccag cactaagtct
     1261 attgaattgt gtttttgatg gttcgtgtat ccacataacc tctccccaat caaatttttg
     1321 tacttcatta tttgactcac taaaatactc cataagctca cctcttagtt tttgtctaaa
     1381 tattttttct tcccttctta tattaaaata atatcatcat cattaacttt cattactata
     1441 aaatcatcat actcttttaa tactcctgta attttagttg cacttagttt actacaattc
     1501 agttttttaa tatattgata aattctataa ttactttttt attttgtaaa atcagtgttt
     1561 tcccaaaata attatatcta taaatgaaag tatttttact gcttacatca atatatgtta
     1621 tcataatttt cctttttatt gcatatataa ataactgtat ataacattgt ccaatactat
     1681 atagaaatac atctaagaaa gaatattatt aaattgttcc aattagtatt atttttctta
     1741 tagaaataaa aaaactgttg gagtctttta gaaaaattat tttttacaga ccttttttgc
     1801 ttagatagtc tatcttctca gctattttgt atatacactc ttctagttca tgattttatt
     1861 ttaatatctt gcttatattg ctgaaataat tgaattcttt tatctatcat attgtctctt
     1921 atatcacaaa catattgttt cttatatcac aaaaatgaaa gcattcttat atcttcaata
     1981 tataaacctg ctttttttaa tcttgtaata aatataggat actctatatt ctcttcctag
     2041 aaataactaa taccacttat ttttctatta tatagaaagt aataaatctt tttcttaata
     2101 aaattcaata gtaagtggaa taaaatttta tatgaataag aacattataa aaatacttta
     2161 tacaatagtc aataaaagat attagcttca aattatgtta aagttgtatt atttaaagtg
     2221 aaaaaacaaa taaaaaagaa ataatagtat aagtagaaaa atatacttaa aacaaggcga
     2281 agcctctctt gtatctaaat caagtagaga attaaatcta aaaaagaaaa taaatataaa
     2341 agatgactcc tgtacattac aagaatcatc ttatggtttc ataatcactc catggttttt
     2401 aactgtctga tataaaggtc tcagttcact catagcatga ccaccttttc cgattgctga
     2461 ccccgtcact tatggacaaa atgtccataa gtgatagtcc tctcttaaaa ccctctaaat
     2521 agatatatct caagcaccgc attgaaacca gttgctggaa acaaactgaa gcgttctgga
     2581 atagtaaaag aaattgacac catatccacg ttactgtcct agttgttact ttgctaactt
     2641 cggagcgatt tcctctaaca atacgcagtc aaattctgcc tttttgctaa gaaatggcag
     2701 atggtctgca tctggtataa aaaaaatctg tttgtctggc gccactattt catcaaaaaa
     2761 tttcttcgct ggaagatagg ggctattata gtcattttct cccatgatgt agtatacagg
     2821 gacctgatag tcaactccat aatttcttat gtcagcttct tccactaaaa accgcaatac
     2881 atcaccttga ttgtcaatcg catttggatt gacaatggcc gtatttttta actctctcaa
     2941 tgaataatac ggggttgcca gtatactgat tatattgccc aaatccatat ttatagaaag
     3001 cccatacttg ctttggtatg ttcttactgt cataagagct gccaaatagc ttggaccata
     3061 ttttgctggt ggatatggtg caatagcttc cagcgccttg atatcttttt gcttccctgc
     3121 agctttcacc atttcaagtg tcttttcata gcccacgcgc tcattgtcca tcatattaac
     3181 ggtctgcgca atcccaatgt acgcgctgta atcctccgga taggtttgca cagccatagt
     3241 tcccagcact gttccccagc tatgacctat aaggataatt ttctccttgt tgaactgttg
     3301 cttcaaatat gctgtcacct catggacgtc ttgcagaaca cgctccgcgc tcatggtctg
     3361 caaaactgtt gccggatcgt tcgcaaagta tgttttgcct acgtttcgct gatcccagtt
     3421 caccacagta aaatcatttt cccactcata ctggtacaga tgtaaaaatg gcatcattga
     3481 attaccagga cccccatgaa cccaaaggat gatggggttg tctgcgtttt gtccccggat
     3541 atataacgcc tgctgtatgc cgccgatctc caccatttcc atgcggtcga tgccaggagc
     3601 ttctatttta tggcttgtac gtataatcgt gggggctatc agacgcaaac cggcaaacgc
     3661 cagaacaaaa acagcaacta gtatgcaaag tgtgattaat atcattttaa gtaccttctt
     3721 tcatgaattt ttttagccat agatatctcc tctaacatta aaaaaagtat gttgctattt
     3781 tagttttgtt actctacctg tcccattggt ttcctttcat aaaaaatatt aattgattct
     3841 ataatctcat cttccaggat atcaactcct atttgtctca taagaaactg taagaattga
     3901 aaccgtttac gaagtgtagg aaagtccccc tgaaatacat ctgtatcaca ccaaaagtta
     3961 aagagcagca aaattacctc tgcgcattca tctgggaact ctgtgatgat gcttctatcc
     4021 tctatgcctt ctcgtaaaac atttgctaat attggtgcta gttttttcaa attatcctgt
     4081 gtaaatgcca gtatgatatg cggactttct atcgcgcttg taatcatgtt actagattct
     4141 tttatcacct catccgttaa atgccgccta actaaacttc taagcttctc ttttgctgta
     4201 agtccatgca tttcaccgag ccattgattt actgtctcta ttatctgttc aaagcgcctt
     4261 tccatgacca cattaaaaat atcttctttt gaactgaagt gatgaaaaat tccgcccttt
     4321 gacattcccg aagcatcaac aatattttgc atacttgttt tgtcatatcc tttttcagca
     4381 aacaactttg cagaaatggt aatgatattt tcaatagctt gctttggatt gttctttcta
     4441 gtcattatat aactccttct tattaaaaac cgactgttgg tttttattat ataagctatt
     4501 cctgcttctg tcaagcaaca ttatgtttat ggcaaaacaa taaggtttgg tggttagaca
     4561 ggtattaatt agactaatgc gactttcccc tagatggaac ccttggtaaa tttgcaatct
     4621 ttaccggctt tgattatatc tgtaagcaag gcaggtatgg ccacacctgc cctgtttgac
     4681 cctcggatat tcattcctat ccctcctctc cttagccatt acaccaaaac gccgctcatt
     4741 gtctccaatg gcggcgtttt aaagatatga tttctcatgc tgctgaaata tgcaaaaaat
     4801 ataagatgcc ctttattatt tttcctacaa tagaaaagaa aagtattgaa gaatgggtta
     4861 atattggagc aaatatgctt tgttttgcac aagatactac ctttatcact tatgttatca
     4921 atcaattatt atctttttat aatgagattg taccaattca aaaacaacat taaattataa
     4981 atctaatttt atattaaaag agactaatat tttatctatc tctattaaga atattttctt
     5041 atgtatcata atattagtct ctcttattat tatatactgc tatcatcatt agttgctatt
     5101 tatactattt ttataatata caaatttatt cagctaacat tttgcctaat tctatacact
     5161 catcttttgc ttcttcattt gggtcttcca ttgctgtaat tccttctcca ccaataattg
     5221 tagcaccagc attttgcata cgttcttccc agtctctcat ccattcacaa tttccccatc
     5281 catatgaacc aaataatcca atctgtttac ctgataccat agattctaat tctgaaacaa
     5341 atggctccat atacctctct tctagttgct ctgctcccat agatgggcaa cctaatgcaa
     5401 atttatcttc ttcttttaaa tcacttggtt tcagtaagct cacatctaaa acttctggtg
     5461 tcttaccttt taacttcaca ccttcagcca caaaatttgc cattttttca gtatttcctg
     5521 ttccactcca ataaacaata tatattttac tcatttttac ctcctgtagt attttttaat
     5581 taatcaatag ttagttatag ctaactaaca tctaaaaaac aaaagatttc tttatctgaa
     5641 atctctttat ttttctaaat atagaatatc ataattgatt ttgaaattca atacttattg
     5701 ataatattta tcatttatat tttatattca ttttataata tcaaaacact gtatcgtatt
     5761 tctaatcttt catagaaaaa tttctaactt atagtacttt tgcatctctt ataaatcttt
     5821 tatatgtata agtacataga aaaacacacc ttttccagat gtgctatcta taatatcgta
     5881 tatttatttt tcaattctta ctagctaata tgtttataaa ttttatcact taataccttt
     5941 atacattgat gtaaaatctt acacctaagt aatttttatt atttgtctcg actctatata
     6001 atatagctat tttatcatca aatcaaaaat tatcttttgc tattattctt gtatttataa
     6061 agatttaaat ataagtatcc atttccttgt tccgttctag gtattccaag cgctctcgta
     6121 cattttatta attgtgcata cagttcagct tcatctaaat ctctttcaaa ctcctctctt
     6181 gcccattctt taattagtgc taatgagcct gatacgtatg gtgcactcat actagtacca
     6241 ctcataatag caagtttatt atccatataa gtcgatataa tatttcttcc tggagccacc
     6301 aaatctattg tagtatttga attactaaac ttttcaacta aatagttttc atttattgca
     6361 cctacttcta ttacctcagc ataactggct ggataactat actcacttgt actagaatca
     6421 ccatctccat tattacctgc tgcacacact acagaaatat tattttttac tgcttgcatg
     6481 acagcatttt ttaacttctt atcatctttg tttcccccaa gagacataga tataatatca
     6541 accttgttat ttacagcaaa gttaatagca ttaattatac tttgatatgt tccagtacca
     6601 tctttattta atgcttttgc tattaataat ttacaatctg gagctactcc cataacttca
     6661 ttattatagt tagatgcagc tataataccc gccacatgag ttccatgacc attaaaatcc
     6721 tcatatatat ttttatttcc attactgtca tcactaaaat ttgcaccacc aattattttt
     6781 ccttttaaaa gaggatgaga tatatcacaa cctgtatcta ttataccaac tacaatattt
     6841 ttaccagtat aaccttcatc ccacattccc ctagcattca tttgttttat tccatatggg
     6901 aatttatttt ttgcacctct tagattttca tttatttcat acggaattag tttcattctt
     6961 ttattcatca tatatcctcc taaaaatttt attgcttata aaaatcatga attttatttt
     7021 ataataggtt tataaatcaa attaaaacct atatctactt atttatattt atgatatatt
     7081 ttttattttt aggagtcata ttttattgta tataaaataa tatttagata actatattaa
     7141 aaaatacatg tttgcttata tacaaaactt actaaaatat cttcaatatt tttgtataca
     7201 aacattatat tttttatata aaaatctcat aaaaattttg attatatttt cataagttaa
     7261 caaatgtggg tataatatga atttacaaaa ttcctctgtt tgtataagtg ctttaccaat
     7321 acactaatat ataatcacat tgaagctata tttgtattta tagtagataa tgtatcaaaa
     7381 attggttatg aatagtaaaa ataccagtta agcatatttt catgtttcta tttttaacat
     7441 taaatttcca atcttcatat gtatattttt aacaaaggtg tttacttatt taaaaactct
     7501 aaaactcttt cattaaaatc ctgtgcttct tcatatgtag cgtgtcccaa tccctcatag
     7561 ataaatattt cgcttttttt aatcttttct gctaaatgaa aagaagcatt atttccaaca
     7621 attttatcat tagcacctcc tataattaag gttggacatg ttattttatt taattcggaa
     7681 aaggcattat gttctatgca agaggtcgct tgaataataa atcgtttaaa atcctttggt
     7741 ttacctactt ttcctaaaaa aggaataaat aattggtatt tttttaaata tctttcagaa
     7801 taggatttct ttgctgtgtc aatcattaaa tcattgtaat tctgcttctt cgccatatca
     7861 atccaactac aaataacatt ttggatagta tcattttgct ttgaagaagt aactgccaac
     7921 acaagtttct caactaactc aggataatca attgccagat attgagctat cattccacct
     7981 tgagaaacac ccattacttc tgctttaata attccaagtt ttttcattat atctgcttgg
     8041 tctttagcca tatcccttgt ggaatatttt tctgtaattt gattttttct actaaataca
     8101 taaaccttat aatcttttgc aaattgctta tatctaaaag caaaagcaat cgcctgtatt
     8161 tttccatgta aaggaaataa accatctcct aatcctggta aaataattag tgtcttctta
     8221 ccactaccaa attcaatata attcactgta ttacttccta tcatcatttg acaacttttt
     8281 gcattataaa acatagtcta ttcctcgctt ttcctattat aattgtctaa ataaatcttt
     8341 aaatttttga ataccatact catcaattag aggaatatcc atttttttta acatgaataa
     8401 taaaaaatcc aacctattac atacttcact gtattcacgt ttaaataata cagggtcaag
     8461 taaaatatca atactaaaaa cgagcatttc agctaattcc tctggatatt cacaagaaaa
     8521 ttctttattt tcaacacctt gtttaattat cttagcaata ttgggtgcaa ctacattgtt
     8581 gagattacga acttcctcta ttaaagcgaa tgggattttt tctaaccagc tacaatgtac
     8641 catttttcta cgtgtgtaat cattcgcact atgaacaacc aattctatta ttttttcatt
     8701 tgctgtaagg gtgtcatcag caacaagatg ctctaaaaaa ttattcattt gtgagtaaag
     8761 ttcttttgtg acacttctca gtatatcttc tttagtttta aagtgatgat acattgcacc
     8821 tctagataga ccagataaat tgataatatc ttgtgtagat gttgaatcat atcctttaga
     8881 cgaaaatagt gaaaatgctg attggataat tttttcttta ctatttttac ttttttcttc
     8941 tctattcata tttccctcct aaaaacagac tgtctgtttg ttttttatta tacattttaa
     9001 tatttattat gtcaagaatt aacttacttt caatttcttc atagttcaga tggaacgaaa
     9061 agcattccac atcaaacttt taaaaagttg tattacctca acaaggtcta aaagatacat
     9121 ctctatgctg tactcgtatt ccttataata gagatgtatt gttttcaaag ataatcaaga
     9181 ttttgctatg tttttcatac aaaataaaaa ggatacctca ttagtgctat aacacctaag
     9241 atatccaatt aatattttct taactttttc taatttacat aaatatagtc tactatttta
     9301 aaactgctcc tgtatttgct gatgttacca atttagcata tctacttaag tatccatgct
     9361 ttattttcgg ttctaaaggt ttaaatttta attttctatt ttctatttca acttcatcta
     9421 cttttaactc taattttttg tctggtatgt ttatagaaat tatatctcct tcttcaacta
     9481 acccgattaa accaccttcc atagcttctg gagaaatatg gcctatagat gctcctctag
     9541 ttgcccctga aaaacgacca tcagtaagaa gtgctacatg cttatcaagt cccattcctg
     9601 caactgctga tgtaggagat agcatttctt tcattcctgg accacccttt ggaccttcat
     9661 atcttataac tataacatca cctttgttta tttttttacc aaaaatagca tttacagctt
     9721 cttcttctga attaaaaact cttgctggtc cttcatgaac taacatttct tctgcaactg
     9781 ctgacttttt tacaacagct ccatttagag caagatttcc tttcaatatt gcaagcccac
     9841 cttggttact atatggattc tttaatgtat gtattacttc ttcattttct atttcacaat
     9901 ttcctatatt ttcccctata gtcttgcctg ttacggtttt gcaatctaaa tttattccat
     9961 ttatctttga aagttcgttc attatagctg gtattcctcc tgccatatgt aaatcttcaa
    10021 tatgatgttt tccacttgga cttaattttg ttaaacaagg agttttttca cttatttcat
    10081 caaaaaaatc taagttaagc tctattcctg attcataagc tattgcaggt aagtgaagta
    10141 ctgtatttgt agaaccagcc attgccatat caacagctat agcattttta aaagcatcta
    10201 ttgttaaaat atctctaggt tttatgtcgt tcttaagtaa ctccataaca tacatacctg
    10261 catattttgc catccttttt ctttctccag aatgtgacgc agcagtacca ttatatggta
    10321 tacccatgcc taaagcttct gttaaacaat tcatgctatt tgctgtgaaa agaccagaac
    10381 atgaaccaca tccaggacaa gagttcatgg ctaacgtttc taattcttct tcacttactg
    10441 ttccatcttt acatgctcca acaccctcca ttgcagagtt aaaatcatat acttttccat
    10501 tttttttacc tggaagcata ggccctccac tcacaacaat acttggtata ttaagtcttg
    10561 cagcagccat taacatacct ggcactatct tgtcacaatt aggtataagt accaaagcat
    10621 caaaaccatg tgccatagcc atagcttcta ttgaatctgc tattaattct cttgaagcca
    10681 gggaataatt cattccaaca tgccccatag ctataccatc acatacccct atagcaggaa
    10741 attctaatgg caaaccacca ctcattctta ctccattttt tgcagcttct gctatttcat
    10801 caagatgcag atgaccagga atcacctcat tttgtgcatt tactatacct attaatggtc
    10861 tttcaatttc ttctttagta agacccattc catacattaa cgctctcttt ggagctcctt
    10921 ctattccttt ttttatatca cttctcataa tattacctct ttcttacttt tttaaatttt
    10981 cataatatta ttataatata tttttctatt aattaattac gaaaattgta aaaaaatatt
    11041 agcatatact aatgaaaatt tattacgatt ttttatcaat atcttacaca attgtattat
    11101 ataatattat cttttcaata tattttataa actactatat tttacaaatt gttgagtaat
    11161 attttatgat ataaatcaga agaggcactt ctataataga aagtgctcaa ctatgaataa
    11221 gattaataat atatttgcct tagatatata gacttttata aatattaaag attaatcaaa
    11281 tctgatgttt gaaactttat cccaattaat cttccttgaa tagtcttctg gatgtgataa
    11341 tctaactata tgagttgaat tatcatagct gtaataaatt gtagtagtac catcaactgg
    11401 attctttttg tatccttgat acacagcact atttggtact ttcttttcaa caaagctata
    11461 tgcagacgaa gtatcaatag tttctatttt attaggaaat ataaacatat aaaaagctac
    11521 tactataaaa atacatatgc atgtaatact ataccattta atccatttca taaaattcct
    11581 cctactttac caaaatatac tattattatt tccttatatt tataatattt atacatacga
    11641 taaaattttt atatccctaa ctatataa
//
LOCUS       DAELJO010000123        11624 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELJO010000123 DAELJO010000000
VERSION     DAELJO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.123"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..115
                     /locus_tag="KPB88_002626"
     CDS             <1..115
                     /locus_tag="KPB88_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0054894.1"
                     /translation="AKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRYFGK"
     gene            375..983
                     /locus_tag="KPB88_002627"
     CDS             375..983
                     /locus_tag="KPB88_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG0054895.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            1310..4207
                     /locus_tag="KPB88_002628"
     CDS             1310..4207
                     /locus_tag="KPB88_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0054896.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            4528..4740
                     /locus_tag="KPB88_002629"
     CDS             4528..4740
                     /locus_tag="KPB88_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0054897.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            4753..4974
                     /locus_tag="KPB88_002630"
     CDS             4753..4974
                     /locus_tag="KPB88_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0054898.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            5074..7230
                     /gene="feoB"
                     /locus_tag="KPB88_002631"
     CDS             5074..7230
                     /gene="feoB"
                     /locus_tag="KPB88_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0054899.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            7256..7408
                     /locus_tag="KPB88_002632"
     CDS             7256..7408
                     /locus_tag="KPB88_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG0054900.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            7588..8298
                     /locus_tag="KPB88_002633"
     CDS             7588..8298
                     /locus_tag="KPB88_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0054901.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKK
                     DSTPSDRMSSGGLRLGID"
     gene            8383..9153
                     /locus_tag="KPB88_002634"
     CDS             8383..9153
                     /locus_tag="KPB88_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0054902.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            9146..9877
                     /locus_tag="KPB88_002635"
     CDS             9146..9877
                     /locus_tag="KPB88_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054903.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            9911..10870
                     /locus_tag="KPB88_002636"
     CDS             9911..10870
                     /locus_tag="KPB88_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0054904.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10968..11564)
                     /locus_tag="KPB88_002637"
     CDS             complement(10968..11564)
                     /locus_tag="KPB88_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0054905.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4473 a         1183 c         2074 g         3894 t
ORIGIN      
        1 agcaaaagaa ttaggattag atgctataca tgatacagta catgaaatgt gtaaagatga
       61 agctagacat ggtaaagcat ttttaggatt attaaataga tattttggaa aataaattta
      121 atataaagta gattttagga gtatctcact tataattttt aaagttatga gtgagatatt
      181 ttttaattat ttgtacttta ttttttattt ctaacgatta tatttttaat aatagagaat
      241 aatttaatag taataatgca caacttttaa aaataaatga ttaatccaaa aataaatttg
      301 attttagaaa agtaaattga catgtaaaga agaatatatt aaactaagta taaaaaattt
      361 gctgttttgt aaaggtggtg gataatctga taaagtatat acataaaagt gatgtagcta
      421 ttgttgtttt gcatgaaatc tatgggatta atagctttat agaagatgtt tgtcaaaagt
      481 attataaata tggatatgat gtattttgtc ctgaacttct gggagaaaaa agagtttttc
      541 catattcaaa agtccaagag gcatatgatt tttttataaa taatgttggt tttgatattt
      601 ataaaaaagt tgaaaagctt ataaatgatt taaaatcaga gtataaatat gtatttgttg
      661 ctgggtatag cgtgggagca actattgcat ggagatgcag tgaagattct ttttgtgatg
      721 gcataatttg ttgttatggc tctcggatta gagattacat ggatgtaatt ccaaaatgtc
      781 ctaccttatt agtatttgca aagcacgatt catttgatgt tgattctatt tcttaccaac
      841 tttctaggaa acaaaatatc caaattgaga ttttagatgc aaatcatggt tttatagata
      901 gatattcaaa taattattct gatattcaaa caaaagtatt tactattaaa gaaaaaagtt
      961 ttctgtctaa atatttagca tgatttagat tattataaaa tatatagaaa gatttaatta
     1021 atctgaaaag tttttatgac actattgata tttaaaatat tagtagtgtt attatatttt
     1081 ttgaaataaa attaatcact agaattatag tgttagttgt gcttttatga cttataagaa
     1141 actaatatta gatagtgtat ttaaatagat aataataaat tcattatagt tgaataagaa
     1201 tattatatga gaatattgca atttgatata tttaccatta caataaaagt agttacaact
     1261 agaaaaccaa ataagttaaa gtaagttggc aaattggagg tttttaaata tgaaagcatt
     1321 taaaaagcac ttcttgatac tttctttgat atttttactt attatatcat gttttaacaa
     1381 tatactatgc tttgctgatg agtctgaaaa ttcaaaaaaa ataagagttg gatattgtga
     1441 tgactacgga ataatatcct catcaaaaga gcattcttat acaggatatg gttatgatta
     1501 tctaagagaa atctcaaaat atacaagatg ggaatatgaa tttgtaaaag ggtcttggga
     1561 agaatgttta gacagactag aaaagggtga aatagactta ctcggacctt tacagaagaa
     1621 tgatgaaaga aataaactat ttaattttcc taaactaagt tcaggatatg aatatagtgc
     1681 actatataca aaggatagta acaataatat gttttatgaa gatataagtt cctttaaggg
     1741 aatgagagta gctgtactta gaggaaactt tcataatact gcatttgaga agtatagaga
     1801 agaaaataat ttttcagttg aatatatata ctgtaattct attgatgaat taattgagtc
     1861 cgtcaatgaa aaaaaggcag atgcatttgt ttgtggaagt attataaatg ctaaaggaat
     1921 gaaaatagtt agcaaatttt cagtagaacc tttttatttt gctactgcaa aagataaacc
     1981 taatttagta aaagaattag attatgcttt aaaagaatta aaaataaatg atatgtatta
     2041 tgagttggaa ttatataaaa aatatttcaa aagagaggtc aataattcca tagcatttac
     2101 tcggcaagaa atgaatttta ttaaagcaaa cccaaaactt actatggtat atgattcaga
     2161 gtggtctcct gtagagtatt atgataaaga aagcaattca tttaaaggta taagttccga
     2221 tttaatgtca attatatcaa aaaaatgtgg tataaaattt gaatatatta aaacaaaaaa
     2281 ttataatgag agtcttgatt acataaaaag tggtaaggca actatgctat gtggctcaat
     2341 caatgaaaat gataaggcaa aacgatttaa tatgaaattg acaaatccat acataaatat
     2401 tccaatgatt atggtaggaa aattggatac caatctcaat aatgatttga atattgcttt
     2461 aactagtagt tataagtcta ttgattctta tatagaaaag agttttgaaa atgctaagac
     2521 aacatcttat aagagtgtag aaagttgtct aaacgctata aatgaaggta aagcaaactt
     2581 aatgatactt agctcttatc agtttgatga acttttaaga gaaggaaaga gtgagaattt
     2641 atctgttata tctgttttag atacaagcta tgggatgcgt ataggggtat caaataaaac
     2701 tgaccctata ttagtgtcta ttttaaataa atcaatagac aaaattactg aagaagaatt
     2761 aagtgattgt atatattcta atactattga caaaccttat aaagtaccat ttggtgtgat
     2821 ttttaaagaa tattcaatac aaattatttc ttttgtatgt attttattct taattgctat
     2881 taaatatata atttataata aaaagaagaa agaggattat ctaaggaaga tagcatatac
     2941 agacccattg acaggagcag atagtataga taaatttaaa ataaattcta ataaactgtt
     3001 tgctaaaaat aatccagaag aatatgcgtt gttttatata gatgttgaca aatttaaata
     3061 tataaatgat atgtttggat atgatatggg aaatgatact ttgatacata tatcaaatac
     3121 tattgcaagt gaattaaagg aagatgaaat ttttgcaaga gtttctgctg accattttgt
     3181 tcttcttatt aagtataaga cagatgatga tattaaaaca aggttaaaca atatttacaa
     3241 taaggttcaa atacttagta atcctaaaat aaactattat aaattgattt tagattgtgg
     3301 tatatataaa ataagtaaaa gtgataatga cataaatact attatggata gagctaatac
     3361 tgcaagaaaa actataaaag gtggtcataa aaattcattt gctttttacg ataaggaaat
     3421 gcacaaaaaa atattaaaag agaaagaaat tgaaaattca atggtagatg ctcttaataa
     3481 tggagaattt attgtttatt ttcaacctaa atatagtctt tcagattatc aaattatagg
     3541 ggcagaggct cttgttcgtt gggataatcc acaaaaaggt ctaataccac caatagaatt
     3601 tattcctgta tttgagagga atggttttat tgtaaatatt gatttctatg tatttgagga
     3661 agtgtgtaaa aagattagag aatggatgga tgaaggacaa aaagtagtac ctatatctgt
     3721 aaacttgtct cgaatgcatt ttgtaaatag taattttata gagaagttta aattaattgt
     3781 agataaatac aaaattccga caagacttat agaattagag ctaacagaaa cagcagtatt
     3841 agataatata gaaggattac ttgatacaat gaacaatttg aaagaaaagg gatttgtaat
     3901 ctctatggac gattttggta caggatattc ctctcttaat cttttaaaag aattgcctgt
     3961 tgacatatta aaactagata gagcattttt tacagaaaaa gatgagagta ataatgaaaa
     4021 aattgttata tctaatgtca ttaaaatggc aaaggaactg aaaatgaaag taatatcaga
     4081 aggtgtagag actatttcgc aagtagaatt tctaaagcaa attgggtgtg atatggtaca
     4141 aggatatttg ttttctaagc caatgcctgt aaaagagttt gaaaaaatcg cttttaaaaa
     4201 agaataaagc aatgtgtaaa taatgacata tataataaag gtgctttaat attagttgag
     4261 tatactattt tagagcatct ttatttttgt attaattttt acgattttat taataaaata
     4321 aatcaaatga gaaaaaaaat caaaagaata caataatcac ttgcaaaaaa tatactattg
     4381 tgatagactt aaatcaaaat taggaattgt aaaaagaagg aaaaaatagg atattatttt
     4441 aatagattaa gatttaaggt attgtaattt atatataaga taacgattgc ttgtagattt
     4501 aaattacttt aagaggaggg atattatatg atgccattga tattatcaaa tacaggtgaa
     4561 gaagtagtta taaagaaaat ttcgggaagt gataagacga ggactttttt aaatagtata
     4621 ggttttgtag tgggtacaaa tgttaaaata gtttctaaga tagatggtaa cttaataata
     4681 gacgtaaaag atactagagt tgcattagat aaaaaaatgg caagtagaat aataatatag
     4741 ggaggtaatg acatgaacag actgaaggat attaagtgtg gagagactgt aaaagtcaaa
     4801 aagttagaag gagaaggagc tacacgaaga cgtataatgg atatggggat tacaagaggt
     4861 gttaatatat ttataagaaa agtagctcct cttggtgatc caattgaggt aacagtcaga
     4921 gattatgaac tatcaattcg taagtcagat gcagaaaaaa ttattgtgga ataataaaga
     4981 ttaattagag tagggttaat gtaattatat aatgagaatg attttctgta tcacaaacag
     5041 tgatgtattt aaataataat ggagggaatg gaaatgtcaa taaaaatagg tctcatagga
     5101 aatcctaact gtggtaagac aacaatgttc aatgggctta caggctcatc acaatatgtt
     5161 ggtaactggc caggagtaac agttgaaaaa aaaggtggaa aattaaaagg aaataaagat
     5221 gtggaaatag tagatttacc aggtatttat tctttatctc catatacatt agaagaagta
     5281 gttacacgta attttatgct agatgataag ccggatgcag taataaatat tgtagatgca
     5341 tcaaatatag agagaaattt atatcttaca actcaagttt tagagctagg tataccgacc
     5401 gttatagctc ttaatatgat ggatattgta aataagaatg gtgataagat aaatattaaa
     5461 gaactatctg aagttatagg ttgcccagtg gtagaagtaa cagcagttaa aggtcaagga
     5521 attatggagg ctgctgaaaa ggcagttgaa cttgcaagta gtaacaataa attaaatttc
     5581 aaattaccct ttgttgatga aagtaaggat gcaatagaaa aaattgaaaa aataatagaa
     5641 gaaaagactc catatataga tgtagaaaca cgctggttag ctataaagct atttgagaga
     5701 gatgaaaatg ttatacaaaa acttgatatt tctaaaacaa tattgaatag tattgaagaa
     5761 ataactagaa attgtgaaga tgaattggat gatgatagtg aaagtataat aacagccaat
     5821 cgttatgaat ttattagttc aataatttca agtataataa agaagaatcg aaaaggtaag
     5881 gaaactgtat cagataagat agataagatt gtaacaaatc gtatactagc attacctatt
     5941 tttgcactta taatgtgggg agtttactat atagcagtaa gttcattggg tactatagca
     6001 actgattgga caaatgatgt actgtttgga gaaattattc aaggcaatgt aagtaatttt
     6061 ctagcttctt taaatgtagc agaatggtta caaggacttg tagttgatgg tctaattggt
     6121 ggagttggag cagtactagg atttgtacca caaatcatgc ttttattctt attattatca
     6181 atattagaag attgtgggta catgtcacgt gtagctttta taatggatag aattttccgt
     6241 aagtttggtc tttcaggaaa atcatttata ccaatgctta taagttcagg ttgtggagtt
     6301 ccaggagtta tgtcaactcg tactatagag aatgatagag atagaaaaat gactattatg
     6361 ttaactacat tcattccatg tggagctaag attccaataa tagcattatt tgcaggggca
     6421 ttatttggag gtgcttcatg ggtagctcca tcaatgtatt tcttaggtat tgcaatgata
     6481 atcatttgtg gaattatatt aaagaaaact agtttatttg caggagaacc atctccattt
     6541 gttatggaat tacctcaata tcatattcca agtgcaaaag gtgttttaat acatatgtgg
     6601 gatagaggaa aagcatttat aatcaaagca ggaacaatta tatttgttgc ttgtggagta
     6661 atctggttct tacaatcatt taattggtca ttacaaatgg tagatgctgg agatagtata
     6721 ttagctagct taggaaatat agttgcacca atatttgctc ctcttggatt tggaaattgg
     6781 caatcatcag tagcaacagt tacaggtcta gtagctaagg aaaatgtagt tggaacattt
     6841 ggagttttat ttggtatatc agatgcaaca gagcaagacc caacacttct tgcttcagtt
     6901 gcaagcatgt ttacagtagc tagtgcgttt gcatttatgg catttaatat gctttgtgca
     6961 ccttgttttg cagctattgg agccataaaa agagaaatgg gttcatggaa atggacatgg
     7021 attacattag gattccaaac tttaacagcg tatataattg ctcttcttat aaatcaagtt
     7081 ggaagtttag ttttaggtac tggaggaagt attgctgggg caataatatc tatatttata
     7141 gcagtagcag tagtctttgt agttttaact tattcaaata aaaatatgaa aaaagaaaaa
     7201 atgggtaaat taagttatat gaaaaattaa ttcatataaa taaggagtga tgaatatggc
     7261 tacatttatt atagcagcta ttgtagtagt attaatggca ctagctgtaa tgtatatggt
     7321 taaaaattca aaaaagaatg gtagcagttg tggatgtggt tgttctggat gctcaagtag
     7381 taagagttgt catagcgcta agagataaat aggataagag cctgataaaa agttgaattg
     7441 ctttattatc aggttcttat tttttgtata aataacaaat ttcatatttg aagtaggtta
     7501 gactatgctg taattttact gtataattat atataaatat atgattagga gggattattg
     7561 ctaaattaag caatataagt gaatattatg tttaaatttg atgttacaaa cgagcttatg
     7621 tgtatagcaa ggggaagtgg taaatttttt gcaaaaaaag gtgctatggt agcctttaaa
     7681 ggaaatttta attttgaaaa attgctgtta ggtccaagta atggcggtgg attgggaaga
     7741 gcattattag gtcatgttca gaggtcattg acaggagaac aaatgccaat aatggtggtt
     7801 gaaggtgaag gtgaaattta cctagctcaa aatgcttacc atgtagatgt aatatcaatt
     7861 gacccaggtg attccattta tgttgagagt gaaaacttat tggcattttc tgaacaatta
     7921 aattacagtg taaaattgat tggttcaggg gtaatatctc aaaaaggtct ttttactaca
     7981 catttagtaa ataaaacagg tcaaaatcaa gatgttgcta taataacaga tggtaatcct
     8041 ctaattcttg aaggaccttg ttgtgttgac ccagatgcat tagtagcttg gactggaaga
     8101 gaaccatatc ctaaagtatc aaaactatca tggaagactt ttattggtca gacttcagga
     8161 gaatcttatc atatggaatt tgttgaacct ggacaaatag tcattataca accatctgaa
     8221 agattgtctg gcttaaaaaa agattctaca ccttctgata gaatgagttc aggtggatta
     8281 agacttggca tagattaaat cttatgatat agcttaatta ttatttaaga tattaaaaaa
     8341 taaaaagggt agcagggata gatatgttaa aaggagtttt atatgtttga gaaaattcat
     8401 aaaaggctta aaggatatta tattttttta ataattttaa ttttatggaa gatagtatgt
     8461 gttttgggta tatggagtag ctatattctg cctcctcctg aaatagtctt agatactttt
     8521 ataaaaatga tatatgacgg ttctattttc ttaaatgttt atgccagtgt aagaaggata
     8581 ttaataggtt ttttaataag ttcattgatg gctataccac ttggagtttt atttggagca
     8641 aataaaaaaa tgtatgaata tttcaaacca ctatttgaat ttatgcgaag tacacctcca
     8701 ctagctttgg tgccaatgct tattttatgg tttggtatag gtgaagaatc taaaattata
     8761 attattattt tggcatcctt ttttccaata tttttaaata cattgaaagg tataaaaaat
     8821 tgtgacaata aattaataga agtaggtaaa tcttttaatt tatctaaaaa gagaattttt
     8881 tataaaataa taatccctaa ttctatgctg gatattgttg ttggacttag tttagggctt
     8941 gggtatagtt ttagggctat aattggagca gagcttattg cagcttcttc aggactagga
     9001 tatttgattt ctgatggaaa agatatgtct agaactgatg ttgtattggt aggaattttt
     9061 gttattggat ttttaggtat actttcagat tatctatttt caaagataat aacaaaattt
     9121 agcaaaggga agcaggttga tttaaatggt tagaagtgga tttttgctag agaatatata
     9181 taagaaatat ttagttgata ataaagagca tttagttctt gataatatat ctttaaatat
     9241 atctagtgaa gagataactg ttatattagg tgaaagtgga tgtgggaaaa caacgctact
     9301 tagaatttta gcagggcttg aaaatgctac tagtggaaac atatattttt ttaataatga
     9361 taaaaagtgt actccaaaag taggaatggt atttcaagaa agtagactta tgccatggct
     9421 gaatgtaagt gaaaatatac ttttacatac tgaaaaagat aatagaaata aagttgattt
     9481 agacaagtat ttaaaaatga tgaagcttga aaagtttaaa aactcatatc caaatgaatt
     9541 atcaggagga atggctcaca gagtaagtat agcaagagca ctttccttca atcccgatat
     9601 tttgctgatg gacgaaccat ttgcagcact agattacttc acaagaagaa aaatgcaaaa
     9661 agaagttgta aatatccata aaaatactaa aaaaggtgta gtatttgtaa ctcacaatat
     9721 tgaagaagct atggaaatag caaagaaaat aattgttttt agtaaaaata aaaggattaa
     9781 acaatttagt gtcgaagatg aatataatag agatttaact aaaaactatt atataaactt
     9841 gaaaaaagaa atattaaggg aattggggga gttttaattg attaaaagaa acaaaaagat
     9901 tttagcagta atgacatctt taataatggt gttggttggg actgtaggat gttcaacaac
     9961 aaatgataag aaagatgaac ctgcaaaaaa agaggctagt ttacctaagg aaataaattt
    10021 aacttatgta aaatcaccac ttaatgttcc tagtatatta gaaaaaaagg atgatttatt
    10081 tggaaaggaa tttaaaaagg acaatataaa agtcaatttc catgaattga caacaggacc
    10141 agagcaaact caagcattag cagcaggtga gctagacttt ttacatgcat taggaggaac
    10201 gtctgcaata atagctgcat caaatggtgt tggtcttaaa attacgaata tttatagccg
    10261 ttcaccaaaa ggatatatgc ttataactaa taatgataat attaaatcta taaaagactt
    10321 aaaaggtaaa aaaattgtag gtccaaaggg gactatactt catcaactgt tgattgctgc
    10381 acttgctaaa gaaggctata ctatagatga tgttgaatat gtaaatatgg atattcctag
    10441 tgctgcttct gcattagaaa gtaaaagtgc tgatgtagca atgcttgcag gtccagtggc
    10501 attgaaaacg ataaattcag gtgccaaaat gattgtaaat ggagaaggat tagtaagtgg
    10561 aataatagtt actgctgtaa gtgatgactt tgctgaaaaa tatcctgaat tagtaaaaag
    10621 atttatgaag gttcatgaag aaactttaaa atatatgaat gaaaacaagg atgaagtgat
    10681 ggatgttgta tctaaagaag ttggtttatc tttagatgaa acaaaagaaa tgtattcttg
    10741 gtatgacttt agttctaaaa taacggacaa ggatataaaa gaattagaag atacacagga
    10801 atttttaatg tctaatggaa tgcaacaaaa gaaaataaat attaaagata tgttgtacaa
    10861 tcaaaattag aatactttaa aatattaaag tggaaaatac acccttattt attatggaaa
    10921 ctgtatggta aaagagggtg tatttttatt attatacaaa aacataatta aaaacagctt
    10981 actattttat ctacacttat gtagtctagt aattttctca ttttcttacg gtgaagtaaa
    11041 aacttcgaat atgtgtcctc ggattgtttt gtgtcaccat aaactttcca atatttaatc
    11101 atagaaactt cataattact atatcccata aatccaatca catctttaag aggatatcct
    11161 aaaaaaatac ctatttcatc aggaaaaaca tcactcttaa gtttatctac taaatgctct
    11221 aaataagcat taacattcgt attttgtttg tatcctaaaa actttaaaaa gtttactgtt
    11281 tttttacatt ttaatttttc tgataatgct tttttattta caaataagat ttttaatccc
    11341 tttttttctt tatcaataac tataaaatct attttcttac atttatatag atatttataa
    11401 atatcattaa ttttttcctc tctatttaca tcagtaaacg aaatatttat tatttcagca
    11461 ggtttagagc ctaaaaagac tggacctaaa acttctaaaa gccattttat atatgatgat
    11521 tcaattttat ttttacaaca aaattgtgaa ttacaacaag acataaaaga taacctcctt
    11581 caaatgaaaa ttaatatcaa gtaattagat tatactatga atga
//
LOCUS       DAELJO010000124        11539 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELJO010000124 DAELJO010000000
VERSION     DAELJO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.124"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(78..764)
                     /locus_tag="KPB88_002638"
     CDS             complement(78..764)
                     /locus_tag="KPB88_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG0054906.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(813..1346)
                     /locus_tag="KPB88_002639"
     CDS             complement(813..1346)
                     /locus_tag="KPB88_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0054907.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1390..2022)
                     /locus_tag="KPB88_002640"
     CDS             complement(1390..2022)
                     /locus_tag="KPB88_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG0054908.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2112..3191)
                     /locus_tag="KPB88_002641"
     CDS             complement(2112..3191)
                     /locus_tag="KPB88_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0054909.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3324..4670)
                     /gene="pepV"
                     /locus_tag="KPB88_002642"
     CDS             complement(3324..4670)
                     /gene="pepV"
                     /locus_tag="KPB88_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG0054910.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4739..6019)
                     /locus_tag="KPB88_002643"
     CDS             complement(4739..6019)
                     /locus_tag="KPB88_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG0054911.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6146..6406)
                     /gene="hfq"
                     /locus_tag="KPB88_002644"
     CDS             complement(6146..6406)
                     /gene="hfq"
                     /locus_tag="KPB88_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG0054912.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6520..7455)
                     /gene="miaA"
                     /locus_tag="KPB88_002645"
     CDS             complement(6520..7455)
                     /gene="miaA"
                     /locus_tag="KPB88_002645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG0054913.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7493..9460)
                     /gene="mutL"
                     /locus_tag="KPB88_002646"
     CDS             complement(7493..9460)
                     /gene="mutL"
                     /locus_tag="KPB88_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG0054914.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNYIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9471..>11539)
                     /gene="mutS"
                     /locus_tag="KPB88_002647"
     CDS             complement(9471..>11539)
                     /gene="mutS"
                     /locus_tag="KPB88_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0054915.1"
                     /translation="NSSEYMVLDMFTRTNLELTQTIRGNKKKGSLLHVLDKTSTAMGG
                     RLLRKYVEEPLINKSKIENRLDVIEEIKDDFILREDLNDILKNIYDIERICGKIAFER
                     VTPKELIHLKNSIEKLPNLKDTINLSNAKILKEYVSEMDKLDDIYNLIDEAILEEPTI
                     TIKDGNIIKSDFSDELKELREISKNGAFLVKEIENREREKTGVKSLKIGFNKVFGYYI
                     EITKANFKQAKLDETYIRKQTLSNAERYITPELKEIEEKILHAEEKIKSLEYEIFVEI
                     RDTIYKNIDRIQKVAKTIANIDVFVSLATVAHINNYVKPAINENNKLDIRNGRHPVVE
                     NIVGEENFVPNDTYLNRGENIINIITGPNMSGKSTYMRQTAIIALMAHIGSFVPAESA
                     DIPILDRIFTRVGASDDLSQGQSTFMVEMNEVSLILKNATERSLVILDEIGRGTSTYD
                     GISLAWSIVEYIQKNIRCKTLFATHYHELTDLEEEFKEVKNYSIAVKEDGEGIIFLRK
                     IIPQGADKSYGIYVAKLAKLPDEVIERAKYILKDLEKNHVYNSVAINGDKENNNIINS
                     NLDEELVKVNQSNFKNKYESLKIEHELIVKDYKHIKKDYDKLNSKFKALNDEVALMKQ
                     NDDKEKINQEDSVKEVALTQISFDSVNRDILSEEILNLDILNMTPLDAINSLYNLQRK
                     AKEIKS"
BASE COUNT         3532 a         2024 c         1184 g         4799 t
ORIGIN      
        1 tccttctaaa taaaaaattt gtctaaagtt tctaaaaaat ttagacaaat cttaggttat
       61 tactctttaa attattttta aattattcca aatacgccaa ataatattgg tataaatata
      121 gctggtaaca aattggctac ttttatattc gttaatttaa gtacatttag accaagtcct
      181 ataattaata aacttccagc tgctgtcatt tctgtgacta caggatcact taaaaatcca
      241 tttaaaaatg atgctcccac acatataata ccttcataca aaaatacaga tattgcagaa
      301 aacattacac ctatacctaa agaagcagta aatataattg aagctattcc atctataaca
      361 gattttacaa ataaagtatc atgacttcct gtaagaccac tttctaatga accaactact
      421 gccatggctc ctatacagaa taataaacta gatgatataa aaccctctgc tatagaatct
      481 tttttatttc ctttagaaaa tttactttgt aaaaaagcac ccaacttatt tacccatttg
      541 tcaatatcta ttaattctcc tatcagagca cctacagcta cggatattat tgtcactaat
      601 gtgttttttc cttctagtgc tcctgatata ccaatgtaca atacacataa ggcaattcca
      661 ttcattatag tatcgcttac tctttgaggt aatcctcctt ttattataag acctactata
      721 catccacctg caattgctag actatttgct attactccaa acattttttc tcccctcgat
      781 tagctaattg aaatttatcc tattattaaa acctaaacat ctatactttg tattggatat
      841 tttaaatatt ccaaaaattc ttcaaaacct tttctagata tatttacagt tttagtattt
      901 atgttaggat gaaaatttaa gtaatcttct ttaattacat ctttatctat gtaaagctca
      961 acttttcttt ctgtatcatt tattaatgaa aaaggattta cacttccagg aagaagttta
     1021 agtacattgt atagtctttc aggtgaagca aacgataatc tagaacttcc tatcttatct
     1081 ttaagtgagt ttaaatcaac ttgtttttct cctttaacta caataagata atacttattt
     1141 cctttggcat ttcttaaaaa taaattttta cattgacacc cctttgttat tccatctaaa
     1201 atgactaatt gctctgctgt atatacagct tcatgctcta ctacatcata ttcaacattt
     1261 aatttatcca aaatatcata tattttagtt tcattcatgc tagattcatt ttcatcttgt
     1321 tcttttttat tatttaaatc actcatactt ttctccttaa acacttcatc ataatatcat
     1381 tgtgaggtat tattctacta tagctattgc tcttactgga gctccatcag catcattata
     1441 cttaaatggt gctgctataa atagaaattt ttctggaaca ttttctaaat ttgtaagatt
     1501 ttctatgatt atttttcctt tttcaaataa aatatgatgc acttcaaata ctgatgtgtc
     1561 atatctatcc actgatagca tatctattcc tatgcctttt atattagtat tagctatata
     1621 gtttgcagct tcttttgtta atgtaggata acctacatag tactgctttt tatcccaaaa
     1681 ttttgaccaa cctgatttaa atattataaa atcactattt tttatttcat cttcatattt
     1741 ttttagatat tctaattcta tattctcatt tcccttttca agttccaata cataagcttt
     1801 cccaacaaaa ttttctatat ccaacttatc tagggtctca cctttagtat acatatgtct
     1861 tggtgaatcc atatgagttc cattgtgaga aaatacacta attaatgttt cttgataacc
     1921 attttcttca ataatagcta ctttcttgat atctggtcta ttaggctctg catatacagg
     1981 catgtcatta tgtgtcacat gagttaaatc aaaaaccttc atctttatat accccctatg
     2041 tttttatttt ctattatata cctaaattca ttttatttct atcagatttt attccattat
     2101 ataccatact actatatatt ttttataaac ctcaagggat ttaccgaaaa atcttggaaa
     2161 gaattatcag taaatataac atcttcaccc aaaaaacgtt tataccttcc ttcttctttc
     2221 tttaaagtaa ttctctgttt ttgtgaatgg ttattaaaaa ccattatctt aagaggaggt
     2281 ttttttctat tagtttgcac tctttttaac atgtatttta tgtgcatatc agcttcagga
     2341 aatgaatatc cacaaaatat taaaaggtca gtatctctta aagttttttc tgtttcattc
     2401 caaacattac ttaaaaatat gtttgacata ttcttaaaat atgttggtgg cactataata
     2461 ggttctgtta actcaccaca ctctaaacat tttgtctctg aactattttc aataagcttc
     2521 atgacaccac cttcatgtgg tgtaagagtt acactattac aaatggggca ataaagccaa
     2581 ttcaatgacc catgtatctt atataatttg attaatggtt cttttggttt tctccaattt
     2641 aactcctctt taaaatttgt aaactccaca ccataatcaa gcataattgg caaatcattc
     2701 ttatctctta atccagtaac tgtattgtct atatgtatat cataatttgc actaataaaa
     2761 gtagtatcaa gaagcaaatc attttccaaa agattttcaa ctaaaagttt atgatattta
     2821 ttatctgttc taggtgtatt atgaattgct tcagccatta gcagtattaa atattgtctt
     2881 aaaaaacgta tactatcact tctgttattt agattttcta gtccaaaatt tctaaatgct
     2941 tcttttcttt gttctgctaa gtctaaaaga cctaaaactt cttcaaaggt aggaaaattt
     3001 gcattatcta tattatcttt tactatatca atattgaaca tctgtttaaa gaaaatatac
     3061 aattctctat tcattttggt attataattt gtgggattta gttttttaaa atattcgcta
     3121 aaaagttcat tttgaatagg tagattctct gctgctgaag ctcctgctcc taaaaatatt
     3181 gctattttca tatagttcct cctaatataa atattactcc tatttttata tccattaaat
     3241 atccatatca tttattttat gatacaacaa taaagctgtc ttttactaga aaaaatccta
     3301 ttacaagaca gccttatatt atattaaatc tttaataatt cgtaaataga aagagcatat
     3361 atttgagtag cttttataat atcctctact attaagtatt cattagcttg gtgttctgtt
     3421 tctggttttc ctggaaaaat agctccaaat gcaacacaat tatctaaagc tcttgcgtat
     3481 gttgctcctc cagatgaaag tggtgttcca tcaagaccag tttcttcttc atatactttt
     3541 ctaagtgtct taacaagaag agtatcttct ggaacataga ttgaatccaa gaaatcatat
     3601 tcttcataat ttaaattgta tttatctgtc attttcttta actcttttac aaaatcatcc
     3661 tttttgtatg ttactggaat ccttacatct attcctgcaa attctttttc gttatcaata
     3721 gttactctac ctatatttac tgttagttta cctgatacat catccttgca attaggaatt
     3781 atattgtttc cattagcatc ttcaccaata acttctgcta aaaacttaat tatattagaa
     3841 tctataccta tattattaag agctatacaa agtctagcca ctgcatttat accagtatca
     3901 cttgctgctg aatggacact ttttccaatt atacagattt tattaccttc aacagtatac
     3961 tcaaaattta atttatcaag ttcttttttt aatttatcag agtattttcc aatatagatt
     4021 gctttacctg gaactgcatt taaagccttt ccaactgaca actctatatc acttttgcta
     4081 tcacaagtca aatgaacttg taaaagacct ttctctgcat ttgttattgg gaatctagaa
     4141 tctggagtaa atccatagtt tggtatttct tcattatttt ctttatattt atttatacat
     4201 ctccaaagat tttcttcatc agccccaaaa atgaatctta tcctcttatt aaagtctaca
     4261 tttaaatcca taagagcttt cactgcataa attgctgata ttgtaggtcc tttatcatct
     4321 tgtgttcctc ttccatacaa tttaccatct tctaaaacag cttcaaatgg agggtaattc
     4381 cagctttcta aatcaccttc tggaaccaca tcaacgtgac caagtatacc tatcattttt
     4441 tcaccttgtc ctatttctgc atatccataa tatccatctt tgtatactgt cttaaatcct
     4501 aaagatttac ataagtctaa tatacctctt aaagctttat ctacattttc accaaatggg
     4561 caattttcag ttgcttcaga tataacacta ggtattttta cactttcttg tattgaagaa
     4621 atcatttcat cttgtagaga atttactttt tctttaattt gctctttcat attatcaccc
     4681 ttcaattttc atcataatat actaataaat attttgacct tttaaaaaaa aaatatgctt
     4741 attttttcag tttttttata aattcaatcg cttttaaagt ccccattttt gaatggtcaa
     4801 atgtaagtcc accttgaaaa taaacattat aaggaggtct tattggagca tctgcactta
     4861 attctataga agaaccttgt ataaatgctc ctgctgccat tatgacttca ctttcgtatc
     4921 caggcatgtc ccaaggaaca ggttttacat aagaatcgac tggtgcagct tcttgtatac
     4981 cttcacaaaa acttattact tcatcagcat tattaagtct aatacattga ataatatcac
     5041 ttcttaagtc atcatatttt ggtaatacat catatcctaa tttttcaaaa gctcttgagc
     5101 aaaagatagc tcccattaca gcctgagata ctatataagg cgccaagaaa aaaccctgaa
     5161 gtacatttct agttgttcca aaagtaagac cacattcttt tcctattcca ggagaagtca
     5221 ttctataaga tataagctca atcaaatctt ttctaccagc tatatatcca cctgtcaatg
     5281 caagcccacc accaggattt tttattaaag agccagccat aacatctgct ccaacatcag
     5341 taggctcttt ggtatctaaa aactcaccat aacaattatc aaccattact atagcttctg
     5401 gtttaacaga ctttattact tctatagctt cttttatatc actaattgaa agtgattttc
     5461 tccaagaata tccttttgac ctttgtatca taacgagttt agttctatca tttattttgt
     5521 ttttaattcc ttctaaatct aagtttccat cctctaaaaa atctacatca tcatatgtaa
     5581 caccatactc tttcaatgaa cctttttctt ctcttatgcc tataactccc tctaaagtat
     5641 catatggtct accagtaaca gataaaattt catctcctgg tctaactata ccttgtatac
     5701 ataaagtaag tgcatgtgta ccatttacaa ttattggtct tacaagagca tcttctgtat
     5761 taaatacttt agaataaatt tcctctattt tttcacgacc aatatcatta tatccatatc
     5821 ctgtagtcca gttaaagtgc atatcactta gtttagattc ttgcatagct tttaaaactt
     5881 tatattgatt atattctctt atttctttta tttcttcaaa cttatctttt atttcttcca
     5941 ttatttcttg cgatagttta aaagtatcat catctattcc atagtagtct tttaataatt
     6001 cttttgtctc attaagcatt ttatcacctg tctctttatt attttattta aacatgtaaa
     6061 ggagactatt ttactaaaac aaataaatta tagtttataa gttagtataa gtctcctctc
     6121 aatcatctta aattaaatta attatctatc tgttgttatt attattgttg ttttggtttt
     6181 gatttgttag attaatatac tttccaggtt gtatagttga tacagcatgt ttataaatca
     6241 tgttttgttg tctattatcc ccttctatta atattatata actatcaaac ccctttacaa
     6301 gccctttaac ttgcactcca ttaactaaat atatagtaac aggtatcctt tctttccttg
     6361 cattatttaa aaacaaatct tgtaaattta aaactgtatt tttcattaga tttgtaccct
     6421 cccaagatta aattgaactt ctaaaatatt cctaccttat attctatata agttatgaca
     6481 aattttcaat attaagttgt atacttaaaa atttatatat tattttatag aatctttaat
     6541 atataagatt atttcatttt ttaattcgtc tatattttca tattggtcta aatcaaacca
     6601 ttttgctgtt ttatatcttt taaaccatgt tatctgacgt tttgcatacc ttctagaatc
     6661 tcttttaatt atttcaatag cttcttcgta tgtatattct ccatccagat actttaatat
     6721 ctctttataa cctatacctt gcattgatat catatctttt ttaaatccca tacttaaaag
     6781 tttttttacc tcttcaacca atccattttt aatcataata tcaactctca tatttattct
     6841 ttgatacaaa tgttctctat ctctatttag aactattatt attggttgat attcttcatt
     6901 aaattttaaa tcacttgaaa aatcattcat tttcttacca cttagacaaa cttcaagagc
     6961 tcttatgacc cttttagtat tgtttttatg gattctatta gcagattctt catcaagttc
     7021 ttttaactta ttatgcatgt aatctattcc cttttcagca agttgttttt gaagttcttc
     7081 tcttaattca ttattggcat cagattgtgc aaaatccata ttatatatta aagagtttaa
     7141 ataaagtcct gtgcctccag ttactaaaac acatttttcc tttttattta tttcatgtat
     7201 atattttttt gctctttgtt gaaactctga aactgaaaat ggatagtcag gcttaacttc
     7261 atctataagg taatgcttca ctccttgcat ctcttcctct gtaacttttg cacttccaat
     7321 atccatatat tcatatattt gcatagaatc agctgaaatt atctctgcat tcatatcttt
     7381 tgctaatttt attgacaaat ctgtttttcc aacagcagtt ggacctgtaa gtattataag
     7441 tggtattttt ttcataacat tcctttccat atattttaaa tatccaaata ttttacataa
     7501 ttcttttaaa cattttttct atttctgtct tagatatttc gaccattata ggtcttccat
     7561 gaggacatgt aaatggattc tcacactttt ctagctgttc caataaactt tttatttcaa
     7621 tatcgtatat tttatcattt gcttttatag cagacctaca tgccatagat gcaaatctct
     7681 ctccttttaa atcataatta cttgtaattt cttctatatt atcaattatt tgaagtataa
     7741 atttctcagt ttcaggtact ccaaatatag taggaacgca tcttaccatt atataattat
     7801 tgccaaaaat ttctagctca aaaccaaatt tcataaataa ctccaagtta ttttcaattt
     7861 gaagcatatc tacatttgat acttcaataa ccactgggtc taaaagtatt tgcatattta
     7921 tatcttgtct atagaacttc tccatatatc tttcatatag tattctttca tgtgcagcat
     7981 gttggtccaa caaatacata gaatcgtctt tgctcaaaat tatataagta ttaaatacaa
     8041 ctccaattac agaatatccg tataaagaaa acttgcgttt tgaactacac aagtattctt
     8101 cttcactgtc cttaatacta tctcctaagt aataatttcc ttcattttta attccatcaa
     8161 cttgaaactc ttcttgtata ttatcatttg cactatcttc acttagataa ttaatgtttt
     8221 ttacatcatc ttctacactt gcatttagga cttcacttac tgattgaaat tcatttattg
     8281 gtttttcact tgataaagta acaacttcaa tgacttcatc cttaccttta gtaatattac
     8341 tatttttagg agtagattct aataaatcat tatttcttaa tttataatct gtactttttt
     8401 ctctactgtt aatagcgatt ctaggttgtg tttttttatc actataagta gcatattttc
     8461 caataagatt tgagtgtatt aatttaacct ttaaaaaatc tctaagttct atatacactt
     8521 cttgttcttt ttcaaattta atttcaagtt tatttgggtg tatatttaca tctatacatg
     8581 atgggtctac ttctatattt aagaagcaca ctgcatgttt acctattgga attatagact
     8641 tataactttc tgttattgca tctatgatta ttttactttt tacaaatctt ttatttatat
     8701 agatatgttg taagttttta tttgaacggt atatattgtt attacctata tatccattca
     8761 ttttaaagtg attgcactta aactctacat ctattatatt ttccgttatt tcttttccat
     8821 atatacttct aatagtgttt acaagttttc catcacctgg agtatttaac atctgtttat
     8881 tgttatttgt atatttaaat tgaatgtttg gattacctat tgcaagttta tttatcaaat
     8941 cacttatgtt tatagtttct gcatgagtag attttaaaaa tttctgtctt gctggtgtat
     9001 taaagaaaat atcttttata atgatggtag ttccattagt agaacctatt ggttctttcg
     9061 atattatttt tccaccttca acatatattt tagttcctat tatttcatct ttagttttag
     9121 tcgtcatttc taattttgat acagctgaaa tagatgccaa tgcttctcct ctaaaaccaa
     9181 gagagtataa atcatacaaa tcatcaattt ttttaatttt actagtagca tgtctcaaaa
     9241 atgatttttc aacttcactt gaaggtattc ctattccatt atctgtaatc ttaattagac
     9301 tttttcctcc atcaattata tctatggaaa ttttatttgc acctgcatca atggagtttt
     9361 caataagttc ttttactaca gaagaaggtc tttctacaac ttcacctgct gctatcttat
     9421 ttatagtcaa atcatctagg atgttaatta tatttttcat ttttacacct ctaagatttt
     9481 atttcttttg cttttctttg aagattataa agagagttta tagcatctaa tggagtcata
     9541 tttaaaatat ccagatttaa aatttcttct gatagtatat ctctatttac actatcaaaa
     9601 gagatttgag ttaaggctac ttcttttaca gaatcctctt gattaatttt ctctttatca
     9661 tcattttgct tcattaaagc aacttcatca tttaatgctt taaatttaga atttaattta
     9721 tcataatcct tttttatatg tttgtagtct tttactataa gttcatgttc tatttttaga
     9781 gattcatatt tattcttaaa attactttga tttaccttaa ctaattcttc gtccaaatta
     9841 ctattaatta tattattgtt ttctttatct ccatttattg caacactgtt gtaaacatga
     9901 tttttttcca agtctttaag tatatatttt gctctttcta tgacttcgtc tggtaattta
     9961 gctaatttag caacatatat tccataactc ttgtctgctc cctgaggaat tatttttctt
    10021 aaaaatatta ttccttcccc atcttctttg actgctattg aataattttt aacctcttta
    10081 aactcttctt ctaaatcagt aagttcatga taatgtgttg caaataatgt cttacatcta
    10141 atattttttt gaatatattc cactattgac catgctaaac ttataccatc ataggtacta
    10201 gttccacgac ctatttcatc taatataact aaacttctct ctgttgcatt ttttaatata
    10261 agggatactt cgttcatctc taccataaaa gtagattgac cttgagataa atcgtcacta
    10321 gctccaaccc ttgtaaatat tctatctaat attggaatat cagcagactc tgctggtaca
    10381 aatgagccta tatgtgccat aagagctatt atagcagttt gtctcatata tgttgattta
    10441 cctgacatat taggaccagt tattatgtta ataatgtttt ctccacgatt taaataagta
    10501 tcatttggaa cgaaattttc ctctcctact atattttcga caacaggatg tctaccattt
    10561 cttatatcaa gtttattatt ttcatttatt gctggtttta catagttatt tatatgtgca
    10621 actgttgcaa gggacacaaa aacatctata ttagctatag tcttagccac tttttgtatt
    10681 ctatctatgt ttttatatat tgtatctctt atctctacaa atatctcgta ttctaaagat
    10741 ttaatctttt cctctgcatg tagtatcttt tcttctattt cctttaattc tggagttata
    10801 tatctttctg cattactaag tgtttgcttt cttatatatg tctcatctag ctttgcctgt
    10861 ttaaagttag cttttgttat ttctatataa tatccaaaaa ctttattaaa tcctattttt
    10921 aatgatttaa ctcctgtttt ttctctttcc ctgttttcta tttcctttac aagaaaggct
    10981 ccattcttag aaatctctct tagttcctta agttcatcac taaaatctga tttaattatg
    11041 tttccatctt ttatggttat cgtaggttct tctagaatag cttcatctat taaattgtat
    11101 atatcatcta atttatccat ttctgaaaca tactctttta ggatttttgc atttgataaa
    11161 tttatagtat cttttaaatt aggtaacttt tctatcgaat tttttaagtg aattaactct
    11221 tttggtgtta ctctctcaaa tgctatcttt ccacatattc tttctatatc gtatatattt
    11281 ttaagtatat catttaggtc ttctcttaat ataaagtcat cttttatttc ttctattaca
    11341 tctaatctat tttcaatttt agatttattt atcaaaggtt cttctacata ttttcttaaa
    11401 agtctacctc ccattgctgt agaagtttta tcaagtacgt gtaataatga accttttttc
    11461 ttattccctc ttatagtttg agtaagttct aaattagttc tagtaaacat atctaaaacc
    11521 atatattctg atgaattgt
//
LOCUS       DAELJO010000125        11387 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELJO010000125 DAELJO010000000
VERSION     DAELJO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.125"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(130..1860)
                     /gene="lpdA"
                     /locus_tag="KPB88_002648"
     CDS             complement(130..1860)
                     /gene="lpdA"
                     /locus_tag="KPB88_002648"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0054916.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2027..3073)
                     /locus_tag="KPB88_002649"
     CDS             complement(2027..3073)
                     /locus_tag="KPB88_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG0054917.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3086..4072)
                     /locus_tag="KPB88_002650"
     CDS             complement(3086..4072)
                     /locus_tag="KPB88_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG0054918.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDEDVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4097..5059)
                     /locus_tag="KPB88_002651"
     CDS             complement(4097..5059)
                     /locus_tag="KPB88_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG0054919.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5097..6050)
                     /locus_tag="KPB88_002652"
     CDS             complement(5097..6050)
                     /locus_tag="KPB88_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG0054920.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARVFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6495..7868)
                     /locus_tag="KPB88_002653"
     CDS             complement(6495..7868)
                     /locus_tag="KPB88_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0054921.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VISASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(7965..9611)
                     /locus_tag="KPB88_002654"
     CDS             complement(7965..9611)
                     /locus_tag="KPB88_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG0054922.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMENESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9633..10799)
                     /locus_tag="KPB88_002655"
     CDS             complement(9633..10799)
                     /locus_tag="KPB88_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG0054923.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYIAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMAKGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
BASE COUNT         3435 a         2241 c         1240 g         4471 t
ORIGIN      
        1 taaaaaatat cccttattat gcaaataaat tatttgcata ataaggggta tggctaagta
       61 agtgttttaa aattttatga tataaattaa tattaataat agacttaaat taataattta
      121 tgtttagatt tattttttag gtaagtgtaa acattctcca tttacatctg ccaaagcttc
      181 cattaaagct tctgaagtac atggatgacc aaatatcaac tcacttgctt catcagtagg
      241 tatctctaac gctttaaatg atgctgcatg atttataagt tctgctacac cacatccaaa
      301 catatgtata cctaaaattt ctccatattt agcatctgct actactttta catatccttg
      361 ttcttgacca gaagctaaag ctcttccatt tccagcaaaa ttaaacttac caacttttac
      421 attatatttt tttctagcat cctcttctgt aataccaact gatgctactt ctggaattgt
      481 ataaacacaa ctaggtaatg cacctaagtc aacttcttta ttcacgccta gagcatttga
      541 agctgctacc tctcccattt taaaagcagc atgagctaac attacacctc cagtaacatc
      601 acctactgca tatatgcttg gtatacttgt ttccatttta ctatttacaa ctattgaacc
      661 tttatctatt ttaatatcta agtcctctat gccagaaaga ttagcttctc ttcctatagc
      721 atatagacat aaatctgctt ttattggctc ttcaccttca atgcacacta atatgttatt
      781 tccttcttct ttaaattcag aaactttctt tttagttaat acatttatac cttttttact
      841 aagagaatat tttaaagatt cgcttaactc tttatccatt cttgggatta ctctatcttc
      901 catttctact atagttactt tagaacctct tgaattgaat atctctgcaa attcacaacc
      961 aatcactcca ccaccaatta ttactaattc ttctggcact gtttccaaat ctaatgcttc
     1021 tgtactagtt attattaaat ttgattctat tccttttata ggtaatatcc ttactttgga
     1081 acctgtagca attattatat tttctgtatc taaaactttt ccatctgata aaattacttt
     1141 atgctcttct ttaacactag ctttaagatt aaacacatct acatctctac tttttaataa
     1201 tccgccaaca ccagctgtaa gttttttaac tactctattt ttatatttta tggccttctt
     1261 aatgtcctgt tctttatcta cagttacttt tacacctctc tttgataatt ggtcaatttc
     1321 ttctaatatt tctgctgttt ttatataagt ctttgtaggg atacaacctc tatttaagca
     1381 agtacctcct agtatatttt cttcaactaa agcaactctt ccacctaaaa gagctgcttt
     1441 taatgctgat aaatatcccc caggtcctcc tcctattacc actacatcat aatcatgatt
     1501 acactctttt ttgtttattt tttcttcttt aaattctata ttttgattct cttcaactat
     1561 atcttctttt gaaacatttc cagataaata tttagctttt acttcctctt gattttcccc
     1621 tttttctgct attacaccta ttactgtaaa tactggtaat acttcacctt cctctttatg
     1681 tatgattaca gcaagtgtac cttctccttc tgactctatc tccatattaa ctttatctgt
     1741 tgttatttct agtataggtt ctccaatctt tacttcttcg ccttcttgct ttagccaaga
     1801 tactatagta ccttcttcca tggctactcc tgcttttggc attattactt ctactgacat
     1861 aggctctcct cctatttatt ttcatatatt ttaattaagc aaatcatatt cacatatagt
     1921 tactgttaaa aataattctt aatatataaa agctttaaat ttactgaaac aattataata
     1981 atataatcta ttcaaactat ttatatcagt aacgaaatat caaaacctat acaagcattg
     2041 ataaaggatt ttctaacaat tcttttaaat cttttaagaa ttttgcagct actgttccat
     2101 ctattactct atggtctgat gttaatgata agttcattat aggttttatt ttagcctctc
     2161 catttactgg aacaaactta tcttgtgtag cacctacccc taagattgca cttgaaggca
     2221 tatttattat aggtgtaaat gtagttatac catacattcc aacattactt attgtaaatg
     2281 tattaccttc ttggtctgct ggcataagtt tccctgtctt tactttttcc gcaagttctt
     2341 tgctttcttt tgctatttct tttaaagact ttttgtttgc attttttact accggaacat
     2401 ataagccttc atctaaacca actgcaatag ctatatttac atctttatat ctaaatatac
     2461 cctcatctgt ccatgctgaa tttaaggctt gatgatttgg aagtatttta gatactgcca
     2521 ttactattaa atctgtcata gttaatttta ctccagttga ttcttttact gtgtcgatta
     2581 actttgctct aagtaccttc aattctgtag catctacttc tatattgaat gtaaatacag
     2641 gagcactaaa ataactttct gacattcgtt ttgctacagt agctctcatt ggatttggct
     2701 tttcaaatat tccttctact gtgttaaaca ttcttgaatt ttcattttca attttcagct
     2761 cttttttctc actagattta gatgtattag ttgatacaat agtttctttt ccagttaatt
     2821 tttccacatc acactttctt atctttccat tatatccagt acctacaata gtttctaaat
     2881 ctatatttaa atcttctgct attcttctag ctaaaggagt agtatttgct ttattatctt
     2941 ttaaataatt taacacatct actaaatgaa tccttccatt ttctccactt ccaatcagcc
     3001 tatctaattt tatattgtct tttcttgcct gacttctagc tgctggtgtg gcttttattt
     3061 tatttgccac catatctatc cctccttatc taacaattaa agattttatt gcttcttcta
     3121 tttcatcaac tcttgggaca actgcttttt ctaactctgg attatatggt attggcacat
     3181 cttttccaca aattcttttt actggagcat ctaaataatc aaatgattca ctttctgtta
     3241 tcagggctga tatttctcca cctagcccac cagtcttagc tgcttcatgg caaattaaaa
     3301 ctctcccagt tttacataca ctctttacta tagtctcttt atctaatgga tataaagttc
     3361 ttaaatctat aatttcaaca tttatatttt cttttgatag agtctctgct gcttcttcaa
     3421 cactttgtaa cattcttcca taagtaatta ctgtaacatc agtaccctct cttttaatat
     3481 ctgcctttcc aatttcaatt acataatcat cttctggcac aaaacctttt tttctgtata
     3541 acaatttatt ctcaacaaat atgactggat tattatctct tatagctgct ttcaacaatc
     3601 ccttagcatc tgctggtgta gaaggtgcaa ctacttttac tcctggtaca tgacaaaacc
     3661 aagcttctaa actttgtgaa tgttgctctg ctgaaccagt tccagaacct cctggacatc
     3721 taacaaccat tggaacttgt gcttttcctc caaacatata tctcattttt gccgcttgat
     3781 ttacaattgc atccatagaa attgttacaa agtccataaa cattacttcc ataataggtc
     3841 ttaagccagt tgctgctgca cctgctgccg ctcctgctat agctgcttct gatataggag
     3901 tatcccttac tctttctggt ccaaattcat ctatcatacc aactgataca ccaaatgctc
     3961 ctccatatat tcctatatct tctcccataa atattacatc ttcatctctt ctcatttctt
     4021 ctgacattgc ttcctttatt gcttgtgcat atgttaattc ccttgtactc atttttattt
     4081 tcctccttta ccattcttag tctgcatata catcctctaa taaagactct atttttggat
     4141 ttgggctatt ttgagcaaac tctactgcat cttctattga ttgctttgca aattcttgaa
     4201 tcttatctaa ttcatcttca ttagatagat tattttctat aagataattt tttaaaaatt
     4261 caatagggtc ttttgctttc catgattcta tttcttcttt tgttctatat acatttgcat
     4321 ctgattttga atgacctaac catctataag ttctactttc aattaataca ggtccttctc
     4381 ctcttctaca cttctcagca gctttttgta ctgtttcgta tacttctatt gggttatatc
     4441 catctattga tattccttct attccataag aagctgctct agtggcaata ctttctatat
     4501 tcatatgacg ttttatgctt gttgacatac catataaatt attctcacag taaaatatta
     4561 ttggcaattt ccatattgaa gataaattta ttccttcatg aaaagtacct tcatttgatg
     4621 ctccatcacc aaaactacat aaaactattt tgcctgtttt tttatactgt tgtgtaagtg
     4681 cagctcctgg agctattgta agacctccac ctacaactcc atttgctcct aagttacctg
     4741 actcaatatc tgctatatgc attgagcctc cttttccctt acaaaatcca gtttctttac
     4801 ccataagttc agccatcatt ttatttaggt ctattcccat tcctataaat tgactatgtc
     4861 ctctatgagt taatgatact aaatctcctt tttctaaagc cattacagcc gccacacttg
     4921 acgcttcttg acctacagat aagtgagttg ttccatgaac catacctctt gcaaaaaacc
     4981 aactaacttt ctcttcaaat tttctagcct gattcattct cttatacatt tcaagcaatg
     5041 tttcttttga tattgacata tacattccct cctgttaact tttatctatc tttctattag
     5101 tcacaatctt ctaataattt aagtgctaaa ctgtgattaa ttgctaaact atttataaat
     5161 ttcgctttta atgatgcctt tattgcctct attttttcat ctcctgaagc gacacctatt
     5221 gttcttttta tcttttttaa atcttccagt ttcactccta atactctttg attaaattca
     5281 agtatagaag tttttccttc tttatcaaag gcttgtaaac aaattgcacc tattgagttg
     5341 tattttttta aatcttctat atctgcttca ttcatgtatc cagaagccat tattgttgag
     5401 tttaaactca taggatttcc tatacccaca attgctatag ttgttttatc tattaaatct
     5461 agtacttctt taattttttc gtctttgcaa agtttctctt ttgtagacaa gtcagacata
     5521 acagctggag catgtaataa tttaaaatca cctccaaaaa ccctcgctaa acttactgct
     5581 atttgatttg catgaatatc tatttcattg tctccaatcc ctcctaatag aggtataaat
     5641 gtaacatttt tacaattact tttttccaca tatcttggta tttcttttag tgttgttcct
     5701 attgaaacac ctattatatc attttccttt actactctag ttaaatattc agatacagct
     5761 cttgctagtt cttgtttttg tgttagtgca tcttgtttat catctactac tattacttct
     5821 tttaatttat attttctctc caaacttctc tctatttttt ggtaatcatt tttcaaaaca
     5881 tcaactatct gtatttttac tattccttgt tcaaaagctt cttttaatat tcttgatatt
     5941 gttggtcttg atattcccaa ttgatttgct atttcctgtt gattcaaatt atcttcgtaa
     6001 taaagtgaac aacacttcat cattaatctt aagtctccaa tttttttcat ttgctttcct
     6061 ccaaagtcaa gcttttcaat tatctttaca tttgttcaat cattttactt aatttcagta
     6121 acattataga aggggttcat ataaaaagtc aactgttttt ttgtataaca aaaaaaaggt
     6181 gatacttttc ttgtaccacc accttcataa atagttgaaa aattgcttct tagtgcatat
     6241 tatatattac ttctaattgt taaatatcat ctccataaat taaaaattaa ttgtccactt
     6301 ataatgcttt aaataacaat aacttattaa atcacttcta aaaaaccaac catttcatca
     6361 tttaaagtct tcttatattt ttattattcg cttctctaaa aattcttcaa taatcataaa
     6421 tatataatag tttttatagg gaacatttac aatagacctt aattaaactt aagactttat
     6481 aagaaacttt ttcattattt agtatttaat tcattaagta tagtactata ttctttatca
     6541 agcttataaa aagacattat gataatttgt aatattgtta taaatatagg taggtatata
     6601 aaacatactt ttattgatac tatagctaaa tcactttgag tagctgatgt tcctacataa
     6661 ccaccaattg ataatactgc acctaatatt gctgaaccta atccatttcc cactttaaac
     6721 ccaaaactag atgcactatt tataagcccc tcagttctat atccagtctt ccattctcca
     6781 tattcaatag tatctgaaac aatagcaaac attgttgatg ctattccagc acttcctatt
     6841 ccctttatag cacttgcaga tattactcct atatagtttt gtccaactat tataaacata
     6901 acatttgacg atattaatat tattgaacct actattaata catttctttt tccaaatttt
     6961 tttataaaag gagcaaccat aaacatagct ataatttggg atatatttga aaccatattt
     7021 ataggaccaa ataatttttc atctcccaaa ataaactttg cataatatac ggatgaacca
     7081 ccatttatcg caaatacaat aaatatgcaa actaaacata atgttatttg tatccagtac
     7141 ttatttctaa ataaagattt aatacctatt ttaaatggta ttacttcatt cctatcttgc
     7201 ttaactgctt ttactctttc cttggttcca gtaaatgtta taaaaaatac cattatagat
     7261 gctattccaa atacaaagaa tgttttagtc catgctgata aattatttcc aaagtattca
     7321 actagaggta gtgtaagtcc atttattagt aaggtaccac aagtggctaa tatcattcta
     7381 aatatactta atactcctct ttcatactgg ttttgagtta taagtgaatt tagagttgca
     7441 tagggtacat tgattgctgt ataaataaca gtagaaacta agttatatgt aataaaaata
     7501 taaattaatt tagaagtgat accaagattt gatggaacag aaaataataa tactgctgct
     7561 atagcaaaag gaatcgccat tctcaaaatc caaggcctag ccttaccgta tttggattta
     7621 gttctatcca ctataattcc cattgttaag tcactaaacc catccagtat cctagataca
     7681 agcataatac tacctacagc tgctgcacta acatctgcat aattagtata ataaaacagc
     7741 aaaaatgctg acatagctga gtatatgata ttgcacccca aatccccaca accataagaa
     7801 attctttcaa aaaatgaaat cttttcatat ctaatcgtag ttgctttaac attaacttct
     7861 gtattcataa taccatactc ctttttgtaa gatttgcctt ctaatgtttc ttatttcaga
     7921 gcataagatt tacctttgct ctgaaaattt tagtaatgat tttattaatc gacatctttt
     7981 gattcatcta ctatatattc aaaaaaatca aaatcagcat agtgtttatg ctttacacga
     8041 tctgcacaag ttaatcctat aaacgtacct gtaaattctc catatttaca gtactcatct
     8101 gaaaacttag ttgtatcaaa tacctttcct attctctggt agtttttttc atcataactc
     8161 cattcaaaat atgacttacg accttgtata tacagacgaa gatatattgg tatatctttt
     8221 attggtatcc tagtatttaa aaattccgtt ttctctccat tttcaagatg tattattgat
     8281 agtgcacttt gacccaatgt ttcactataa tactttctta aatttatata attcatatta
     8341 tcataataca ttatcaaacc cgcactatgc tgatgtacct ctgggtaaaa ctccatctta
     8401 gtggttattc ttgcatatac tgaagttaat tttcttgcca atatgctcac tttatttaat
     8461 gatgttcttg attcttgtcc tctaatcctt acatatcctg gtctagactt aacatctgca
     8521 aatgataatg gtgaaatcct aggagcatag tactgtaaac ctaattcatt tgaatcaaag
     8581 tcatcaaaac ttggtataga tgaaactaaa tgttcttcta gtttactttc tgatacgtat
     8641 tcttttgcca aattactttc attttccatt cgaagccaat tatcctttgt ccatttcatt
     8701 ttttgaatag ctgtttctct acctaaagta caccttaact ctggtacaaa tggtctagat
     8761 gttagatgaa ctaaatatac ttcacctgat gttgtttcta tatagcttcc atgaccagat
     8821 ttttgaagtt ttgattctgg gttatagtat ttaggcttta gatggtcagg gtcatgtctt
     8881 tcataaaaat cgcctggaat tgaagttaca attggattca ttgaatcttt ttcataaggt
     8941 ccccatatat ttttagccct tcccatagtt acactatgac catatccagt tccaccttct
     9001 gcacacatta tataatagta atctccacgc tttgttatat gtggtgcttc aatacaacct
     9061 ctatcagttc cccctgacca tattctcttt ggatatccta ctatttcttt cttttttgta
     9121 cagtattcca ctaaacatat aacaccaggt ttttcatatc cttctcttgt ctcccagtct
     9181 aatgatgcaa tccacttttt accatcatca tcatgaaaaa tagacgcatc aaaacctgat
     9241 gagtgtagat atactggttc actccactct ccctttatat cttttgatgt aattaaataa
     9301 ttatcaacat caaaatatct tgcattcata gagttcataa ttccataaac tatataaaat
     9361 aaatcttctt cttcacaata agtcaaacag ggtgcccaaa ttcctttaga tgaaggaagc
     9421 ttcttcaaat ctatttttgt ttcatctgtc aaaatatgag tatataactc ccaatttttt
     9481 aagtctttag agtgataaac aggtatacct ggaaaccatt caaaagatga aactacaaga
     9541 taataatcat ctccctttct acaaatacaa ggatctggat taaaacctgg aagtataggg
     9601 tttttaatca tatcttttcc tccaatttta gtttaatatt ctataggata ccattcttga
     9661 gctacacatt tgactaggtc ccaactatcc catcctatcc aaccttcttc attaactgta
     9721 tctagtaata acttataatt ccaatagtag tgaccacttc catttctcca tgcatcaagc
     9781 tgtgcttttg ctatcttcct atataattct cttttatcgt cttttgataa tttatctata
     9841 tttgattcaa tcccatttag aggtgattga cctccatttg tatctatacc acaagcatat
     9901 gaattaaata agctccactc cccacatata acaggaaaat atttttgcat ttgtaaaata
     9961 tctttagcat aattttctct aatatatttg agatagctat caatactttg ttcacatcca
    10021 tctgcttcag ctaacattaa atattggtgt gtatctagaa caacattttt aaattcttcc
    10081 tctctcataa agtctttcca tgcttttaat tcaaatgcat catgaaacac tataacgttt
    10141 tcctctctca tataaggacg cattacctta tatgctttta tataaaaatc acgtaaaaac
    10201 tctaatgaat taggtttgct cccctttgcc atctccttat ctacagcctt aaaccgattt
    10261 ggaacattca ttatgttcca cattctttct gttataggct catttaaaat ttggattcca
    10321 tacaattcat gcctcatccc ataacgtttt gcaagtcttt ctaatacatt taatgtaaac
    10381 gctatatatt ctggattttg tgaccagcta catatacctg atattcctcc attatcaaat
    10441 ccattttggc ttcctggaac tgtatgtaag tctatcaata tactaagccc atacttatct
    10501 gcccaagcaa atgctttatc aagttcttta acacatccta tgaatggttc acaatctcca
    10561 aaaataaagt atggtactgg tatacgaact gaattaaatc ccatagattt aatcgttgca
    10621 aaatctctct ctgtgatgta ttcactacga tgagtcttaa ttctagattc atatgcctct
    10681 cttgaaagct gtcttggtaa atagtattca tcttcagctt ctgtgccttc aaataaactt
    10741 gaggtcatcc atttttctaa aactaaccaa cctcctaaat ttacaccttt tatatacaca
    10801 taaaatcctc ccctcttatc ttatttaaat aggtatattt tgcacttata ataaaataat
    10861 ttttttaagt tattttaata tacagttatt atatttgatt ttatccgttt tcttaagtat
    10921 gtttattatc taatttatta gtctatatta tctttttaat attgaaaatt ataaatttac
    10981 tatttataat ttaaaattat aaggaggata ttattatgaa cagaaataat ttttcacatg
    11041 aaataattga acctattgat aatttagatg ttaatttcaa actatttgat taaaaaatag
    11101 ctacgcacac tcgtgacttt agtcatgagt tagtagttaa aatagtcagc atatagggaa
    11161 acatatatgt agagatagga tagaacctat ccaacactac ttgaattgct ggaaacccct
    11221 aaagctaacc aaactaaaac ataaggatga aataaaccta agtgtgaaag ttacgaaagt
    11281 agaaaaaatt ggttagatgg tataaggtta aaccctaagt actttaaaat gggcaatcag
    11341 caggtaaact ccgaatagga gaaacttcaa cgactattcc tcttgag
//
LOCUS       DAELJO010000126        11367 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELJO010000126 DAELJO010000000
VERSION     DAELJO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.126"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            130..447
                     /gene="flgB"
                     /locus_tag="KPB88_002656"
     CDS             130..447
                     /gene="flgB"
                     /locus_tag="KPB88_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG0054924.1"
                     /translation="MNTYDIIKMALDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKIDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            455..859
                     /gene="flgC"
                     /locus_tag="KPB88_002657"
     CDS             455..859
                     /gene="flgC"
                     /locus_tag="KPB88_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG0054925.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            869..1186
                     /gene="fliE"
                     /locus_tag="KPB88_002658"
     CDS             869..1186
                     /gene="fliE"
                     /locus_tag="KPB88_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG0054926.1"
                     /translation="MPITQINNLIQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTV
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1211..2761
                     /gene="fliF"
                     /locus_tag="KPB88_002659"
     CDS             1211..2761
                     /gene="fliF"
                     /locus_tag="KPB88_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG0054927.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVVAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPINGGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGAVAVVAIGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2774..3844
                     /gene="fliG"
                     /locus_tag="KPB88_002660"
     CDS             2774..3844
                     /gene="fliG"
                     /locus_tag="KPB88_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG0054928.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDIKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLQSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDDISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3837..4568
                     /locus_tag="KPB88_002661"
     CDS             3837..4568
                     /locus_tag="KPB88_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG0054929.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFKDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQRA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKYNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4572..5891
                     /gene="fliI"
                     /locus_tag="KPB88_002662"
     CDS             4572..5891
                     /gene="fliI"
                     /locus_tag="KPB88_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG0054930.1"
                     /translation="MYKNNISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            5922..6368
                     /gene="fliJ"
                     /locus_tag="KPB88_002663"
     CDS             5922..6368
                     /gene="fliJ"
                     /locus_tag="KPB88_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG0054931.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYNKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEHLKEQNTNDEFASMSYLRNRQVL"
     gene            6396..7601
                     /locus_tag="KPB88_002664"
     CDS             6396..7601
                     /locus_tag="KPB88_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG0054932.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KGRREISKQNISSDKPENSDNECHLDEDKSNHLKDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANTDHSNDIE
                     NLDIMKLRELLENNADEDIEVISKTTENDIKSIDLYSFDSNRMDNLNSNKQRDDSSDI
                     LEKLAGVNGNKSGNFSQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDNRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7619..8227
                     /locus_tag="KPB88_002665"
     CDS             7619..8227
                     /locus_tag="KPB88_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG0054933.1"
                     /translation="MYGIATVLTGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKENENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8254..9249
                     /locus_tag="KPB88_002666"
     CDS             8254..9249
                     /locus_tag="KPB88_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG0054934.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAAAGPLQGVKGGRLVNGDGYMLVGQIMTGKYDANK
                     GTFTPDAKPAAGWPNEPVVIPLQSISPKDGTVQNVLEYNISKDGTIEFLLSDGQRTQF
                     VYDIPDNSEVGVVPNPAIATDSGKLQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQI
                     AGATGNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9262..9465
                     /locus_tag="KPB88_002667"
     CDS             9262..9465
                     /locus_tag="KPB88_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG0054935.1"
                     /translation="MIRVTDLNGRRYILNSELIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9465..10283
                     /locus_tag="KPB88_002668"
     CDS             9465..10283
                     /locus_tag="KPB88_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG0054936.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMEIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIIKSNKNTERAMSNGA"
     gene            10273..10968
                     /locus_tag="KPB88_002669"
     CDS             10273..10968
                     /locus_tag="KPB88_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0054937.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIANALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLKQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMIKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10980..>11367
                     /locus_tag="KPB88_002670"
     CDS             10980..>11367
                     /locus_tag="KPB88_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG0054938.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTAENLKNIKEEIKNTLNI"
BASE COUNT         4771 a         1234 c         2245 g         3117 t
ORIGIN      
        1 cattattata ttaatgtaat ggaaatgtga ttaatttaaa tgaataaaga agtcattttt
       61 gtaatatagc aacttcattt ttttattaat aaacaaaata gataaaatat attttgagga
      121 ggagtgagtg tgaatacata tgacattata aaaatggcat tagatgcaac taatttaaga
      181 agtcagacaa tagccaataa tatagcaaat ataaatactg aaggatataa gagacattat
      241 gtaacttttg aagaaacatt aaatgatgct atgcccaaaa tagaagaaaa aatagacaat
      301 tctaaaggat taagagtgga tggtaataat gtagatatag aagaagaaaa agtaaatcaa
      361 gcaatggcaa cattacaata taatgctttg ataagttttg caaatggaaa aatagcaatg
      421 accaaatcaa taataagtgg gaggtaatta gaagatgagt gtgttcagtg gaatgagaat
      481 atctgctagt ggattaacag cagaaagttt gaaaatggat atcatatcat ccaatgtagc
      541 taatataaat gctacaagag gtccaaatgg agaagcatat aggagaaaag ttgtttcatt
      601 tgaagaaaac tatgataata aattaggtat gctaggagta aaagttacat ctgttgaaga
      661 tgataaatca cctcttaaag aagtatatga cccatctcat ccagatgcaa agccaaatgg
      721 atatgtagaa tatccaaatg taaatatatt ggtagaaatg acagatttaa taacagcttc
      781 acgagcatat gattctaacg tagatacatt gaatgctcag aagtctatga tatcaaaagc
      841 tttggaaata ggaaagtagg gataaattat gccaattaca caaattaaca atttaataca
      901 aggtgcaaag ttagataatt tgattggaga agcaaagcca ttaactctta ataataaaga
      961 agaaaaaaag aattttgaag atgtaataat agatactgta gataaagtta attttcaaca
     1021 agtaacagca gatgatatga aagaaaaatt tataaaaggc gaagatgttt ctatgcatga
     1081 agttatgtta ataggtcaag aagcacagct atctatgcag tatttaatag aagttagaaa
     1141 taagattcat gatgcatatc aagaaatgaa taaggtacaa atttagcctt ttgaacagga
     1201 gtaataatca atgatagcca gttttaaaaa taagattaaa gagcaagttg ctaaatctag
     1261 gacaaagata agttctctaa aaaagggaac taaaatagca ttaggtatag gggttgttgc
     1321 tatatgttta gcgctagtat ttacagcaat atctgcgagc aaaaataagt atgcagtctt
     1381 attttcagaa ctagatgttc aagatgctgc agatatatca gcagaattag agtctaaaaa
     1441 gatagatact aaggtaaaag ggagtacaat ttatgtacct agaacagaag ttgataagtt
     1501 aaggctggag ttagcaccaa gtcttacagg tggttctaaa ggatttgcac taatggatga
     1561 tagttcatca ataggactta ctgatgaaga gtttcagatt aagaagcaaa gaatgataca
     1621 aggtgaaata gaaaaaacaa taaagagttt tccgcaagtg gaagatacta gagtacatat
     1681 aacatttgga gagtcatcag tatttgagaa ggaggctgtt ccagggagtg cagcagttta
     1741 tttgatgcta aaaccaggga ctactttaga aaaaaaccaa ataaaatctg ttatggcact
     1801 tatatcagga agcgttacaa atataccaga gaaaaatgtt caagtcattg ataacaaaat
     1861 gaatttatta tctgaaggta ttttagagga tgaagatgtt gatgagaata atcagccaat
     1921 aaatggtggc attagtgttg ataaaaataa ggcatctgaa caagctttga ataaacaact
     1981 agaaaagtct attttagaaa tgctagagcc aatatttggt aagggaaagg taaaagctac
     2041 agtaaatgct gatttaagtt ttgatacagc tgaaaaaact gagataaaaa ttgaccctga
     2101 taaagttgca ataaaggaga caaggtcgaa aaattcatct aaatctgcta caaataatgt
     2161 tcaaggtgta gatgctaaca tgaataacca aggaaataac aatggcacta atacagaaga
     2221 tagtttagat gagagttttg agtatgaaac aggtaagatt gaaactcaca ctatagttgc
     2281 acctggagaa cttaatagat tgacagcttc tgttgcaata gatggaaaaa tagctaaagg
     2341 tgttcaagct gacgtagagg atattgttaa taatgctata ggtatggatg gagcgagggg
     2401 agatactctt agcgttgttt caatggtatt tgacccagaa gggaaggcag aagctaaaca
     2461 agagctagaa gagattaaaa aagaaggatt taaaaagaac ataataaaag cagttattgg
     2521 agcagtggca gtagtagcaa ttggagctat tggatactta atatataaga aaagaagtga
     2581 acaagaagat atggattatg aagatgaaga ttatatttct aaatctgatt ctgttgatag
     2641 aaaagataat cctctattag gagatgaccc agaactgaca ttagaggaag ctgttaaaat
     2701 gtatgcagaa gaaaagccag accaagtaac agagataata aagacttggt taaatgagta
     2761 agggggatag aaagtgtcca cggaaattac aaatgttaat gcgtttgact taagtgatat
     2821 aaatgaaagt gataagaatg agaaagtaga aggtgagggt ttaaaaaaag ctgctgtatt
     2881 attaatgaca ttaggaccaa atgtgtctag tgatataata aaaagccttc ctgataaaca
     2941 agttcaaaaa ataggtttgg aaattgcaaa tatttcttca atagcttcga aagaaagaag
     3001 agaaatctta aaagagtttg tagaaataaa tagggcaaaa gattatgtta tagaaggtgg
     3061 gcttgaatat gcaagagagt tgttaagcaa tgctttagga aatgctaaag caaacaaatt
     3121 acttgaaggt atatcaataa atacaagtac aaaacctttt aaacatatca taaaactaga
     3181 tataaagaaa atattagaaa tacttaggaa tgaaagtgca caaactatag ccgttttact
     3241 tgcacatatg gagccagaaa atggagcaga agttttatca tacttagatt ttgatttaca
     3301 aaatgaagta gctatgagaa taagctcaat gggttcaata tctccagaag taataaaagt
     3361 aattgaaagt tcattagaag ataaattaca atcaataagt ggtgatgatt ttaaggatta
     3421 tggaggagtg tctaccttag ttcaaatatt gattaactct gatagaaaaa cagaaaagaa
     3481 tattttggga actattgaag aacaagaccc agatttagca gctcaaatta aatctcatat
     3541 atttgtcttt gaagatattg tcaagttaga tgatataagc atacaaaaag tgttaaaaga
     3601 agtcactatg aaggatttag tatatgcctt aaaaggtgtg gatgatgata tttcagatgc
     3661 tatatataga aatcaatctt ctagagcagc agatgcactt aaagaagaaa tggaaatggt
     3721 tggtgtcata aaagttacgc aggttgaggc tgctcaacaa aagatagcaa atatagtaag
     3781 aaaacttgaa agagaaggtg ccataactat tgtaaggaat tttggtggtg actttgttga
     3841 ataataagat tattaaaagt aaaaaagcat catataaagg agtgctagat ttaaccataa
     3901 tagataaagt tagagtggga gaatcaaatg taaataaaga aaatgattgt agatttaagg
     3961 atgaggtaat aaaagaagct atagaatctg ctgagataaa aaagaatgag atattagaag
     4021 aagcaaaaat gaatgcacaa aatatagaaa agcaagcata tgaaaaaggg tatagtcaag
     4081 gtcaaaaaaa tggatatgaa gatggatata aagagtcata tgatgaatat acccaaagag
     4141 ctaaagatga agcagatttt ataaaaaatc aggcagactt aatactatta caagctaatg
     4201 agaagatgac tgagtacata aaagaaaaaa ataaagagat tgtaagactt gctattaata
     4261 tggctagttc tgttctaaaa aagcatttcg aagatgaaga atctatgagt gatttgttaa
     4321 agcaaattat tcaagattat gaaggaagta gttcatttat agtaagatgt aattctttct
     4381 ataaatataa tatagagaaa gagtttgaat tattcaaaga ggaaacatca ctaaaatcaa
     4441 gtgtatttgt tattgcagat gatagtatag ataaaggcaa tgctatcatc caaaaggaaa
     4501 atggaagaat aatagttggt attgattgtg ctatggaaaa actaaaagaa gaattgcttg
     4561 gtgaataaag tatgtataaa aataatatat ctatagattt tgacaaaata aataagaaaa
     4621 tagaaaattt agaactcact atatcagaag gtagagtaaa gaagataata ggacttactg
     4681 tagaagttga aggaataaaa gctttcgtag gggagttatg cgttatatac aatcaagtca
     4741 ataaaccagt gaattgtgaa gtagtgggtt ttaaagataa agaagtcata ttaatggcgt
     4801 tgggagagct aacattgata gctcctggtt gtaaagttat ttctaagggg atacctttaa
     4861 gtgttatgtg tagtgataat ttactaggta aagtacttga tggtcttgga aatcctatag
     4921 acaactcaga tgctgtttta ggagatagat acaacttgaa caatgaacct cctgacccaa
     4981 tgaagagaaa aaagatacga aatataatgg aaacaggtgt aagagcaata gatgcattta
     5041 ctacttgtgg tgaaggacag agaatcggca tatttgcagg aagtggagta ggtaagagta
     5101 cgactctagg tatgattgct aggaatgcaa aagcagatgt aaatgtaatt gctcttattg
     5161 gagaaagagg aagagaagta ctagatttta ttgataaaga tttaggtgaa gagggaatga
     5221 aaaaatctgt tgttgtgtgt gcaacatcag ataaagcccc tctggttagg ttaaaaggtg
     5281 ctcttactgc tactgcaata gcagaatatt ttagagatca aggtaaaaaa gtaatactta
     5341 tgatggattc agtaactaga tttgctatgg cacaaagaga agttggtata gcaataggag
     5401 agccaccagc ccaaaaagga tatacaccat ctgtatttgc cattttgcca aaacttatgg
     5461 agagaacagg gacatcagat aaaggttcta taactgcatt ttatacagta ttagttgatg
     5521 gagatgactt taatgagcca atagcagata caacaagagg gatacttgat ggtcatatag
     5581 ttttatctag agacttggca aataagaatc actatccttc tatagatgta ttgaacagtt
     5641 taagtagact tatgaatgag attgcttcaa aagaagacat aaaaatagcg tcttttgcga
     5701 gagatatgct tgctgagtat agagaagcag aagatttgat aaatataggt gcatatgctt
     5761 caggtacaaa caaaaaaata gatgaagcaa tctattatca tgagcatatt attaattttt
     5821 taaaacaagg gataaatgaa aagagttctt ttaatgaaac tataagtagt ttgagaagag
     5881 tttttgaata aacaattaaa aaaatatttg aataggttgt gatggaattg gataaaaatt
     5941 ttaagtttag attacagaaa gttttagact taaaaataaa agatgaagaa gagataaaaa
     6001 tggaatttgc taagattcaa caaaaaaaaa tagatataga gactaattta gaaaacttag
     6061 aaagcaatta taacaaatat tctataagta aaaataatga tagtattcaa aatcaaaaaa
     6121 taactattaa ctatttgcta gctttaaaca attctataat ggatttatca gaagagctgg
     6181 ataagtctac aaatgaactt gaaaaagcta gaaaacagct tataagcaag cagatagaga
     6241 gaaagtcatt agaaaaatta aaagaaaaaa aatatggaca gtattataaa gaagaacatt
     6301 taaaagaaca aaacactaat gatgagtttg caagcatgag ttatttgaga aatagacaag
     6361 ttctataaaa tagtaggaag tgaggatatt attgtatgaa gatacaagca gattcttata
     6421 atattattaa agatattggg aatagaaaaa gctcatctaa gaataaaaat ataaaaaata
     6481 ataaatttga agataatcta tataaggcaa cagatcataa aaaggttaaa ggtagaaggg
     6541 aaataagtaa gcaaaatatt agctcagata aaccggaaaa tagtgataat gagtgtcatt
     6601 tagatgaaga taaaagtaat cacttaaaag atgaaaatac atatgaaaaa gattgtagta
     6661 agaataatga agctttacag caattactga atcttttaaa agataattac aaaaaagata
     6721 aagatataaa cactatattg aataatatag aaaaactaag tatatcagat gaattaaaac
     6781 aagaacttaa gcttaataaa aatatattaa ataagattgc aaatcaaaat tcagagctaa
     6841 ataaagccaa tacagatcac tctaatgata tagaaaattt agacataatg aagttaagag
     6901 aacttttaga aaataatgcg gatgaagata ttgaagtaat tagtaaaact acagaaaatg
     6961 atattaaaag tatagatttg tatagttttg atagtaatag aatggataat cttaactcaa
     7021 ataaacaaag agatgatagt tctgatattt tggaaaaact agcaggggtc aatggaaata
     7081 agagtgggaa ttttagtcaa gtaataaata aaagcgttga tatgaataaa attaaaaatg
     7141 ataatgtcaa tttaaaacag atagaatcaa atgtaatgga tgactctata aaagctatta
     7201 agcatatgaa aacaaatgat atacaagaat tgacaattaa attaagacca aaagaattag
     7261 gagatatgaa catacaattg ttaaaagatg gtgaaagtat gagagctgtt gtgactgttt
     7321 tcaataaaga cgtctttgac tctataaata aaaacatagc agatttaaag caacatcttg
     7381 aacttacaaa tgtaaatata aaagatgtat cagtgcaaat gcacagcgat aatacaaata
     7441 catctgatac ctttgataaa gcatttgaac aacagaatag acaaaataat caagaaaaca
     7501 tgagtaagca gaatacaaat gataatcgta acaatgtagt catagaagat gagattaaag
     7561 aaaatattat tgatgatgat agagtagatt tactagctta ataaggggga agtattgcat
     7621 gtacggaata gcaacggttt tgactggaac tcagggaaat tataggtcaa ataatttaag
     7681 ccaacttgaa agaaatatta atgatattgc caataaacaa gttagaagta aaggaaatca
     7741 gacagtaaca gacaatggga ctccaattat aggacaaggt gatgatgctg ataaagactt
     7801 gtttatgaag atacttgtag cacagatggg taatcaagac ccaatgaatc cgcaagaccc
     7861 aacagaatat gtgtcacaat tagcacagtt tgcaacttta gaaaagatgt cagctatgaa
     7921 tgagaagcta gatgtacttt tagccttaag taattccact ctaataaact ctgctttgag
     7981 tatggcaaca tcattaattg gtaaagaagt agaagtatct ggggaagaga aagaaaatga
     8041 aaacattgta ggagtagtaa agtccacata tataaaagat ggggaagtac acttagaatt
     8101 agaagtagat ggaagtgaca aaactgtaga tgttaaatat gaaaaattac ttaaaatagg
     8161 agaggtagat gaaaattcag gagaagaaaa tgactcttca gaacacgaat ctccagaaga
     8221 agcataatat taaatgagaa aagaggtaaa ttcatgataa aagcaatgta ttcaggtgta
     8281 agtggaatga aagcaaatca aacaaaacta gatgtaatag gtaacaatgt agccaacgct
     8341 ggaactactt cttttaagaa aagttcagct agaataacag attcatttta tcaaactgta
     8401 ctatatgcaa gtgctccaac agcggcatta gggggaacaa acttaggaca agtaggtgta
     8461 ggtagtaaga tatcaagtat aaataaagat atgacacaag gaaatgtaca accaactaat
     8521 agaccatctg atttaatgat agatggagat ggatatcttc cagtagtaag acaaggtaca
     8581 gttatgtaca ctagagatgg ttcttttaac ttagatacag gatttaaagc agcagcagga
     8641 cctttgcaag gtgtcaaagg tggaagactt gtaaatggtg atggatatat gcttgtagga
     8701 caaataatga ctggaaaata tgatgcaaat aaaggaactt tcacaccaga tgcaaaacct
     8761 gctgctggtt ggccaaatga accagttgtt ataccacttc aaagtataag ccctaaagat
     8821 ggtacagttc aaaatgtatt agagtataac atatcgaagg atggaacaat agaattttta
     8881 ttaagtgatg ggcaaagaac tcagtttgtg tatgatattc ctgacaactc tgaagtagga
     8941 gtagtaccaa atcctgcaat agcaactgac tctggtaaac ttcaaaaagt acaaacatat
     9001 gcattccaaa atccagcagg gctagatgca gaaggaggaa acttatttaa accatcagca
     9061 aactcaggag acccacaaat agcaggagct acaggaaaca taatacaagg ggcaatagaa
     9121 gcttccaatg tagatatagc agaagaattt acagaaatga taatagcaag taggtctttc
     9181 caagcaaaca gtaaaataat atcgacatca gacgagatac ttcaagaaat aataaatctt
     9241 aagagatagt aggtacatat tatgataaga gtaacggatt taaacggaag aagatacata
     9301 ttaaattcag aacttatagt gagaattgac tccgtgccag aaagcaggat tctccttaca
     9361 acaggagaaa aaaatcttgt aaaagagtca gtagatgaga tagtagataa agtaataaag
     9421 tataaaagaa aaataaatgt aggttatatt aggagcgaaa aataatgaaa aaaacagatt
     9481 cactgacatt tattggacta atagtatcta cagtattagt tttagttggt gccgcaaaag
     9541 gaagtagttc agggttaaag aatttttttg atgtttcatc aatattaatc acagtgcttg
     9601 gttcatttgg agctctaatg attacattta ctatagatga tataaaactt ataaaaaatg
     9661 cattacaata ctcatttaag acaatgagtg tatcaaagtt agatttacta gaacaattta
     9721 aaacactttc taaaaaagct agaaaagaag gtcttttatc aatagaaagt gatgtaatgg
     9781 aaatagaaga tccatttatg aagaaaggat tagagctatg tattgatgga ttggaaatca
     9841 atgagataag gtccatctta gaactagaga tagattctgt tgaagataca aataacagat
     9901 actctaaact atttaaaact tggggaacct ttgcacctgc ctttgggatg ttgggaactc
     9961 taataggtct aatacaaatg ttagctgacc ttacatctcc agacttaata gcatctggta
    10021 tgggaaaagc attaattacg acattttatg gttctttact tgctaatatc gctttgaacc
    10081 caattgcata taatatagat gagaagactg aaaaagaaat ttatgtaaaa gaaatgatgt
    10141 tggaaggtat aataagcata caatctggag agagcagtat agtagttgag gaacgtttag
    10201 ccacatacct ttcaaacaat gagaagttag aaattataaa atccaataaa aatacagaaa
    10261 gagcaatgag taatggcgcg taaaaaaaat aagaaaaagg atgagataaa tccagatgca
    10321 tggttggcta cttatgcaga tacgataaca ctgattctta cattttttgt gttattgtat
    10381 gctacatcag ttgtagatat ggaaaaattt aaaaatatag caaatgcgtt aaaaggtcag
    10441 ttttctggaa caagtatgtt cagtacaggt tcaagtacaa gtgagaaatc tccaatagag
    10501 agtgttttag tggataaaaa tgataagtca aatttaaaac aggatttaaa agataaagtt
    10561 aataattcta acttacaaaa tagtgtaaca gtcaaagatg atagtagagg gattttacta
    10621 gagctagatg attcaatttt atttgactct gggatttctg aattaaaagc agatagtaaa
    10681 gccactctta ataaagtata tgacatgata aagatgataa aaaataagat agttatagaa
    10741 ggccatactg ataatgtacc tgtaaaatca agtacacatg aatccaattg ggatttatct
    10801 tctagtagag cagtaagtgt tgtaagatac tttgtagaag aaaagggcat ggaccctaag
    10861 ctattttcag caacaggatg tggagagtac caacctttgg tatcaaatga aacagcaaag
    10921 ggaagagctg agaatagaag agttaatata cttataacta ttgactagga ggaagaggtt
    10981 tgttcaaaaa taaaaaaatt attataatta ttttgatact gattattttc tcagcaggag
    11041 caggtttttt tatattaggt gaaggtggaa ataaacaaat tgacaagatt gcaggtttgt
    11101 ttattccaac aggaaaaggt aaagatgaag taaagaccga aaatcttgac ttagaagaga
    11161 tggtattgaa gctagctgat gaaggtgtaa gatatttaaa agtatcaata actgtatctt
    11221 atgattcttc atataaagat atagaaaaaa atacgccttt aattagggat aatattataa
    11281 attgttttat ggcaaaaaga gcagatgatt ttactgctga gaatttaaag aatataaaag
    11341 aagaaattaa aaatacatta aatatac
//
LOCUS       DAELJO010000127        11306 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAELJO010000127 DAELJO010000000
VERSION     DAELJO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.127"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            398..2893
                     /locus_tag="KPB88_002671"
     CDS             398..2893
                     /locus_tag="KPB88_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0054939.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            2908..8118
                     /locus_tag="KPB88_002672"
     CDS             2908..8118
                     /locus_tag="KPB88_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0054940.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYSKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELERGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFASLKMENSEDISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVVLTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8343..10442)
                     /locus_tag="KPB88_002673"
     CDS             complement(8343..10442)
                     /locus_tag="KPB88_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG0054941.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTIGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIILKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(10485..10880)
                     /locus_tag="KPB88_002674"
     CDS             complement(10485..10880)
                     /locus_tag="KPB88_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054942.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNNTCTTE"
BASE COUNT         4293 a         1317 c         2051 g         3645 t
ORIGIN      
        1 ttaagcaaat aaaaataaaa tattcaagct ggtataggtg taaaacttaa gcagtagtat
       61 aggaatgttt aaattgatgg tacaaatata attaaaaatc tatatgtaat aaccatagga
      121 aaataatgga catataatat aaaaaacaac tgtattacat aaaatacaat aaaataagcg
      181 cattaaataa aataactgtg ttgataaaga ttatattata caacacagtt attttaataa
      241 acaaagaaga ataattattt agtgaaaatg caaatttagt gaaaatgcaa atttattaaa
      301 aataaaaaac aaatttaaat atagtctaaa aactttgcaa tctacttaag ggattaaact
      361 ttatttaaat aaaatatata accaaggggg aaaataaatg ccagaaaata acaatcaact
      421 tttagaagaa ataacactct tattaaatga aggaaaagta gcaataaata aggctgaaaa
      481 tcaaaattta attctaattc ttggtgggac aggatgtggc aaaagtactg caattaacta
      541 tcttgcagga tgtgtaatga tggaagtaga agatgaagat actggtatat catatattga
      601 ttgcttagac cctgtggcag agattggttc aggagctgtt tctcaaactt tatacccgaa
      661 cattgtaaat atgagaaaac caatagaagg aatggatgca aaattatgtg atactgcagg
      721 ttttagcgac aataggggag tagcccatga tattagtgca gcagtttcat taggtgaagt
      781 atttaaaaat tctgcatcta tatgtgcagt aattgtattg ataccagaag cagatatatt
      841 agattctaga cttacaaaag tactagacct gttccaacag ttaaatatgt gtcttcataa
      901 agaaaatttt aagggtagta taactttttt catttcaaaa agtttcttag gaagaacaga
      961 aaaacaaata tataatgtta taaaaagaaa atatgaacaa ttatgcgaaa atttagacag
     1021 taatatagag gaaaaagctt ggttatttga agaattgcta aaagataatg gaaaaaatat
     1081 tcatctttgt aagccacttg catcagatga gagggaaggc ttattaaagc aagtactaga
     1141 attagatgaa gttaaagata agacatcagc attccaatat ccaattagtg cagatgcaag
     1201 attagtttta gatacgttga taaaagaaat tcaaaacaat atcgtatcta gcctagaaga
     1261 ttatgttaag gcgtatacag aagattttat caatattatt ggaagtataa caatgattca
     1321 agaaatagca aaatatgatg aggttgtaaa agtatttaaa aactggttgg aaagtacaac
     1381 aagtcttact atagagagat ttatcaacgt attagccaaa caaagtggct tattcttaca
     1441 agatagttgc atgaaattac aaaataagat aaaaggaaac ttagatataa ttgatgcttt
     1501 aagtaaatat acagaacatg gaactgggga aattttatta ccttctgtac atatggaatt
     1561 tattatagaa aaggcaagta agaagataat ggaacaaaac ttcattataa taagaaagaa
     1621 tttagaaaaa gttttagttt cttatgaagt gcaaaaacag gtatttacat taccaaatag
     1681 agaaaaatta atggaagttg caaataaaaa ggattatgta tatagtgtaa gtgattttaa
     1741 ttcaattttg acaaatgtta cagttacaca aaatgaagag ttagaagaca gtataacaag
     1801 attaagtgca tatggagaag aaggtaatga gataattaga gaatttattg gtaaatacat
     1861 tttaaatcca tttatagtaa aaacaattaa tattaatgat aagaaaggta ttgaaataag
     1921 agcattaatt ccaaacttag taattagcag tgtattggaa tatttcaaaa atagaaaagt
     1981 agatgacaat gtttctactt ttatgttctt tgctaaagat actatttata tggatgataa
     2041 ccttggaatg aactttgcat cagagaaaaa tattattctt gcttctaata atcttgatgt
     2101 aggtagtaat gtaaaaatta atgtaagtgg aaagagcgga gaacttgtag ataattctat
     2161 gtatgggaaa aatggtgaag atggtagaga tgcaggtaat atttacttat atattaaaga
     2221 agaaattaga aattatcgtc taacactgat tgcaaatgga ggaaatggtt ctaaaggaat
     2281 agatggggaa aatggtgaaa caggagcttc tggaagtaat ggagctgatg caggatatac
     2341 aagttatttc cacggacaga tgtttaaagg gtattttgaa aaaggtacaa gtggcaaaaa
     2401 aggtgaaaaa ggtggagctg gtggaaatgc tggaagaggt ggaaatgctg gtaagaaagg
     2461 taatatttac ttcatttcag attcagagga acaaaaaaat agaataatta atcattgtga
     2521 tataacagtg aaagatggag aagcaggtcc aaatggtaga ccaggtttag gtggatatgg
     2581 aggaagtggt ggctatcacg gatataatac actttcattt tctccaagtg gtatagataa
     2641 gactgtatac tatacaggat attttacaag ttgggattat aagttagata gtataattaa
     2701 atatcatata gaatttagtg gtggaagctg gaatgaaggt aaaacaggac cagcaagaga
     2761 atacgctgaa tatggagata gaggagacaa tggaggattt aatgctccaa acactaagtt
     2821 atcaacagct gtttcaaagg tagaagagaa tcttattaaa gaaaaatatc aaggctattg
     2881 ttccgaaata tagaaaggga tggatgtatg gcaaataaac tttatagtga aatcgtaaac
     2941 cttttggaag aaggaagaga cgagcttaga aaatatgact taaaagaaaa gtcaattttg
     3001 ttattcttag gtgcttcagg cgttggaaag agtacttgta taaattattt aaaaggctgt
     3061 gtgatggaag aaaaaacgga tgaagagact ggacaaatat atataactgc aaaagattca
     3121 gctgtcgaaa ttggaaatgg tgtatattct aagactttat gtccagaagt tgtagatatt
     3181 gcaaatagag atttttcatt atgtgattgc ccaggttttt ttgataaccg tggagcagag
     3241 tatatgattg ctggtgctat gcttgtaagg gaaactattt ccacatcttc aaaggtgaaa
     3301 gggatggtag ttattttaga ctatcaatcc ctactggact ctagacatac attattgata
     3361 gaaacaggca aaaatttgaa agatatgctt ggtgattatt caaagtataa agaaaatttc
     3421 ttctttctta ttaataaaat acctaaaaca ctaattggta atgtaacaga agaaaaaata
     3481 agaaatatat tggaaaaagc aaaagaagac ttagatagtc aagagaaaga ttacgacggt
     3541 tgtaaggaat tagtgtactt attaaatgta gcattatcag atggtgtatc tattaaatta
     3601 tgtaatccac tagataaaca gccagaaaag ttgttgaatg aaatgaaaaa tctacctgaa
     3661 acatttgaaa aaacagattt gggattgatg cttactgctt cttgtaaaat agaaatacaa
     3721 aaagctgttg atgtaaccta taaagaccta gaaaaacttt tagttcctta tatagaaagt
     3781 gttaaaaaag gtatggaaga ttctatttcc attctatctt ctataaatag tctaaaagat
     3841 aaaaagattc aattagataa gatgtataca gatttatgta gtaggactaa taatatgaaa
     3901 gagcttatgg agtttgtaca agaactttca tatggtgatg gcgtacagaa agtgaatata
     3961 ttagaagaaa cacgtcttct tttagagtta aatagttact gtagtgagac tcatattgtt
     4021 ggtctaagtg agttgttaaa atcattgcta aaaaatactt tagaagatac attaaaagat
     4081 atcaaatatt ggatagcaga agaagaatat agggaagaag aagccaaaaa gcttgaagat
     4141 gcagagtata ggatagagaa gattcaaagt tgtacatata taaaatcata tgagaaagaa
     4201 aatgtacttt ctatatatat tcctatacct aaagtgaatt tatcggattt gactaaaata
     4261 atatctggtt atcaacttaa tattttgtca tctatatata tgtatggaga ttgtgagttg
     4321 aaaattgact gtaatatgga gaaagaagtc ttctcaggta agaatattgt actacttaca
     4381 gaaaaattac atataccaga agggacttat tcaatcaacg tatctggtaa ggatgcagaa
     4441 aaattggttg gagaaagttc tatttacgac ggaatgaatg gaagaaatgg caataatgca
     4501 ggtacttcag gtgatatcat attatctttt aaagaaatag aaaaaagagg agaacttaaa
     4561 ctttacgtag atggtggacg tggtggaaat ggacaagatg gaaaaaaagg agatacaggg
     4621 ataactggag aagctggggc aaatgctaaa ataggttcta aaaaagagtc aaaagaattt
     4681 gctaatgtta aaggtacaaa atactatgtg gtaaaagcag aaaaaggaaa acaaggtgga
     4741 actggaggca caggaggtaa tggtggaaat gcaggtcttg gaagcaagtc tggacaggtt
     4801 tatctccttg gaaaagctcc tttattaaaa agtgctataa gtaaaaaaga tggaaatcat
     4861 ggtttagcag gaaaaggagg ctctggagga gctggtggtg caggaggtct tggtggaatt
     4921 agaggtatat atgaagaaac aataagatat ataggtacct ctcctccaat ggataatagt
     4981 gatagaaact actattattt tgatagatat gtgtttaatc atgctttaaa tcagaagcaa
     5041 gcagcagatg atggagagaa attaaatact ctttatgaag aacataagga ctggacaatt
     5101 agcaaacgtg aagattcagg ttcagccgga gcagagggaa aagccggtca ctctggagat
     5161 gcactaaaag aaaaggaatt gttaaagcag gagtattctt tgtcaataga tacattcttc
     5221 tatgaatttt tcgagagatt tggtttttct ggaattaaag gttatagaaa tcttagactg
     5281 ttgtgtgcag taggaatttt agaagctgat attttagaac tttcacatat ttatgaaatt
     5341 tttcatgcag ttgagaaaat agagatgcaa ttaaatagag aaatacttca gaagtactat
     5401 agttttgaga agaaaaataa agcagtactt tctttggatg atatgaatac aaagaaagaa
     5461 ttttataaag aagcaaagga aatctatgaa taccttataa ataaattgat gcagtctgat
     5521 agaatagaat cttttgtagg aaaaaatcag aattttagcg aatggagtat gattttattt
     5581 gcacttgagc agaagacagc attttatgtg cagatgattg aagaaagtga aattggagga
     5641 aatcgtgtta cagatattcg ttctttgaca gattatctgg ataagacctt aagagaattg
     5701 gaacgaggga agaaagatga gttaaaagaa ggatatttca atacatataa ggaaagtatc
     5761 ctaactcaaa ttgaatctgg aaatcaacaa atagaggttt taaaaaatga acttgagaat
     5821 aaaatgcttt ctgttaatgc aaaaatgaac ctgttgattc aagaaattat aaagatgaaa
     5881 gaagatgaac aagaagacat agaggaacta aaaaagaaac aggcaaaact tgaggaacaa
     5941 atgaaaacac agttaattct ttctggaata caaagcgcat tgagtttact gactgcattt
     6001 agtggtgttg gtcaagcttg tctaggggca tttgacagtg caaaatcttt gaaggaaaat
     6061 ctttcatcta aagaagagga atacaaatgg actaacagga tggttaattt ttcagatttt
     6121 aaattggatt ctgatggtat gagatttgta cttggagaac agctaagtga agatagaaaa
     6181 aatgctttag ataaaatgac ttataatttg gcaggtgttg atgaagaaag aagaactaaa
     6241 tttgaccaaa ttaaggagat tatagttaag tctatagatg atgataatta tgcagacaaa
     6301 gctgacgata ttcaaagagc aaaagagggg ttaagtgaaa gcgagtataa ggaaatttac
     6361 acaaaaatcc agaaaaaaga aataggtatg ttggaggcaa attacaataa taaacagcaa
     6421 aaagataagt tagctgcact agagaaaagt aaacaagcta ttgaaagaca gcaaaagaca
     6481 tctaaaatta aaaatgcatt gagtattgga agttctgctt gttctcttat aaatactgcg
     6541 attagtggtg ttaataattg tcttaatgta aaatctagtt ttgagagtaa aatagaggta
     6601 gttaaagagg caattaaaga taaagaaaat aatattaaga aattagatga attgaatact
     6661 caaatggtgg aatttaaaga taaaactttg aaagaatctg ttataccatt tgtagaaaaa
     6721 ttcgatagta cagttaaaaa tcaagatatt tttgaaaatg agataagtaa attaaagatg
     6781 aaggaagttt taactgaatt aagtacaaag tttgcaagtt taaaaatgga aaattcagag
     6841 gacatttcta taatcttcaa aaaagtacag gatatgatgg aaactcaaat tatcattact
     6901 gataagattg ataagaaaaa agataatctt gcaatgggag aattgattta tcaaatgtca
     6961 gatagtggta atcagatgaa tgaatatcag aaaaaactta tccatcaaat tgaattaaat
     7021 caggttagat atttgtgtca ggttgaggca gcagcttttc aactatggac attcccattt
     7081 ggaagcaata tagtaaatat attaaataga tgccatgtag ggattacaga agatgaaaac
     7141 gaggttataa atcaggcgaa agagcaaaat agacagatta gagagtttct tgaccaagat
     7201 gcttaccaat ggcaaaaaac agataaccat attttgaagg tagtattaac aggcagttta
     7261 gatgggaaat cacgtgtggg atttgaaaat acatattctc tatatgattg taatgaagaa
     7321 gaagtgaaaa agcttttgac aggagatgaa attagttttg aagttccatt aaataaaaac
     7381 tttgatgcca taaaatatgt aaccatgtat gcaaatatcc cgaaccttag acgaaatatt
     7441 ttagaagata gtttaaaagt aaaggtgcat attcatttat atgatagtgg atacttctcc
     7501 ctatatgatg ataaaaaaga tgtctattat caatatgtat ttccacaaaa ctatataaat
     7561 attacacatg gtcttgatgt taaatacaat gattcagatt caataaactc aatatggtct
     7621 tcagtagaag ataaaggaat tgaaaagttc aaaaatgcag gattaaactc tggtagaagt
     7681 ccatattcta tggctaagat ttctttgaag gcacatttta ataggaagct ttcttatgat
     7741 tcacttacaa atgagcagaa gaagaaacta gaatcatact ttgataaacc tagtaagtat
     7801 gcatggagcg atagtataga tatattgaag agtctagggt atattgattt gaagggaatt
     7861 ttgtcaaaga gttggtatga aatcaagaaa gatatagata gtgatgggga gattatgaag
     7921 catttttcta tattacaagt tgatttattg gaaagaatta tttatgagca gactagtata
     7981 ctaaaggaaa atatacctaa tttgacaatt gaattggtag gctgtggtat ttatgtagag
     8041 tctgaattta attatctaaa gcaaaatgat ttaaaatgct atgaagaaag attggttgaa
     8101 aatacaatta ttggttgaaa atataattaa tatataaatt ttaaagaaat ttacaaaagg
     8161 ggatgtttca aaatgaaaat ttgaatcatc tccttttttg taaaaaatag aaataagttg
     8221 agtttaaaat ttgtaaagta tgctttatat aattttgatt agaaaaaagc taattttaag
     8281 acatattctt tttaaaagat gaattataaa ttttttatat catatgtttt atgtaatgga
     8341 gattaatggt agttataagt attggtgttt tccaataaat ttgtcatact gtttaaactt
     8401 attccgacag tagatgcgtt gtgtaaaaag gcagatgtac ttggtctaac aaatccaccg
     8461 acaccaagta aaatcagacc taaattaaag ccaataataa acttagaact taaatcaact
     8521 cttttcataa gcttattact aatctgcttt aaaatgatta aattattcaa attatctgct
     8581 gatatggtaa tatctgaaat ctctcttgct atttcagcac cttcactcat tgcaattccg
     8641 acatctgatt ctgaaatagc aggagaatca ttgattccat caccaatcat aattactttg
     8701 cgacctttta gtttttcact tttaacaaaa ttggctttgt cttctggtaa gacttctgaa
     8761 taatattcat caacaccaac tttacttgct atagatttag ctgttttttc gctatctcca
     8821 gtcatcatca caatcttttt tattccgcat tctcttaaat ttgaaattac atattttgct
     8881 tcttttcgta aagggtcatt tatgcaaata actgctgata atttacctga tattgccata
     8941 aataggtgtg aatactcatc tgatagattg ttgtacttat cctcctcacc gtctgggata
     9001 gtacacttct catcttcaaa tacaaaatgt ttgctaccta ttacaacctt tttatcatca
     9061 acttttgttg aaatgccatg agcaataata tattctacct ctgtatgcat ttctttgtga
     9121 ctaagccctt gtttttcagc ttcgtaaact actgcatttg ctatggagtg tgggaagtgt
     9181 tcttctaagc aagctgcaag tcttaacata tcctccttat tataattttg gaatgtaatt
     9241 atatctgaaa cagttggttg agcttttgtt aatgtgcctg tcttatcaaa tacaatagta
     9301 tctgcacatg caacaccttc aaggaatttt cctcctttta cagtaatgtt gttattgtta
     9361 cattctctca tggctgatag tactgaaata gggatagaga gttttaatgc acaagagaaa
     9421 tctaccatta atatagagag tgattttata gggtttctag taattgcata ggttaatata
     9481 cttcctaaaa agctgtatgg aactaatgta tctgctagat gttcaaattt actctctaca
     9541 gaagatttta gtttttcaga ttcctcaatc atagttacta ttttctggaa tctagtagta
     9601 ccagcttttt ccttgacgca cataacaata ttgccttgct ctattacagt tccagcatat
     9661 atgtaagatc cttctttttt gttaacggca agggattcac ctgtaagtga tgcctgattg
     9721 accattgctt ctcctgatac tataactcca tccaatggaa tcatatttcc tatagttaca
     9781 cttactaaat ctccttcctt tatctctgaa attggaatta agatttcagt gtcacctttt
     9841 cttaaccata ctttttcaat gtttagtgac atactttgag ccaagtcgtc tatagatttt
     9901 tttctagtcc attcttctaa tagttcaccg ataccaagaa gaaacataac tgaaccagcc
     9961 gtattgatat cttttctaaa aatagaaata cctatagcag tggcatctaa aacagaaact
    10021 tctatttttt tatttagcag ggaagagaga ccttttttaa aatattttat agaatgaagt
    10081 ccaattaaaa aaaatcttaa ggactttgga aaaaatattt tggagatact tttgactagc
    10141 agtttatttg ctaatttgtc tctataataa ttgtttaatt ccctgctggt atgttcagga
    10201 actaggtttt ctattttgct attgttaaaa ctgaatttag tgatttctct tattatgtat
    10261 tctctttcac cagtgtaata aatgactgca tcaccagttc tatcgtaaac ttttgctttg
    10321 gtaataccat ttatagaagt taaataatat aagagagtat cggactgctt gttgtttaat
    10381 ttgccaagtt gtaagtcaaa gcgtatacga cctcttattt cgtgttttat ataaaaattc
    10441 aatttacttc ctcctgatat tattaatcat aataatcatt aaatttattc tgttgtacaa
    10501 gtattatttt tttcttcaat tacgttatct ttgatttctt cacatttaat gctattagaa
    10561 ttagtatctt ctacagtttt tatagtttca gttttaagaa cttcttgttc gcatttaaga
    10621 gtatcttgta caacgtttac cttagatgta gataagtctt cttgctcgtt gatttctttt
    10681 gcttcagcta aaatatcttc tacattttct tgaattttcg tagttgtttc aagaacagaa
    10741 tctttaactt tcaatgctcc tgctgttgca cgagtgcaag tttttttaac acaatcactt
    10801 gaaagtatct taacacctac agtaccaaaa agtactcctc ctaaaaatat ccctgttttt
    10861 ttcattttta aaatattcat tttacattat cctttcattt agttagattt tgtttgtact
    10921 tatgtataag gtaacaaatg ttaaaaatag agtatgtttt acaaaattag tttagagagt
    10981 ttagtaattt tatttgattt atattagtaa atttacatga attttacatg tgtagtttat
    11041 atagttgaaa taattcttat atagcttata tatgcagttt atatatctag cttatgtatg
    11101 taatttatat atctagctta tatagttgag gtaatacttt atctagctta tataatttat
    11161 ataaatttat aaccgaatac tacacagttt atacataaca tattgtttga gcttatctag
    11221 ttcatataaa tttataactt atataaattt tttgtacttt gtattttaga aagttcttga
    11281 tatttagtcc aaaaacttta aaatac
//
LOCUS       DAELJO010000128        11267 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELJO010000128 DAELJO010000000
VERSION     DAELJO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.128"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            34..453
                     /locus_tag="KPB88_002675"
     CDS             34..453
                     /locus_tag="KPB88_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054943.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKAIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            478..5535
                     /locus_tag="KPB88_002676"
     CDS             478..5535
                     /locus_tag="KPB88_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG0054944.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALKLIFTSEQVEELCQLIKR
                     KIDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEDVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     CCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFNARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDLNLENI
                     ASSLIKSSNSQKCLGGLEIANLLKDNTDRQELYNKLILLIEEIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSHSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENANYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRQTSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRS
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKSIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFIFNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(5721..6458)
                     /locus_tag="KPB88_002677"
     CDS             complement(5721..6458)
                     /locus_tag="KPB88_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0054945.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6477..7412)
                     /locus_tag="KPB88_002678"
     CDS             complement(6477..7412)
                     /locus_tag="KPB88_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0054946.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7620..10196
                     /locus_tag="KPB88_002679"
     CDS             7620..10196
                     /locus_tag="KPB88_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0054947.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10208..10879
                     /locus_tag="KPB88_002680"
     CDS             10208..10879
                     /locus_tag="KPB88_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0054948.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            10906..11247
                     /locus_tag="KPB88_002681"
     CDS             10906..11247
                     /locus_tag="KPB88_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054949.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         4383 a         1462 c         1666 g         3756 t
ORIGIN      
        1 aaatacataa ttaatatttt gcgaggtgat tttatgcaaa tttattttag agttaaggct
       61 gttggcaaac gcaaacctat gctagaactt accccttttg atgtatctga caacatcaat
      121 tgcctaaaag aagctatctc tgaaattgta tctaaaaatg ttcttgaata taatgagaaa
      181 atcccagaaa aagcaataat aaacttctta actaatgaag aaattgaatc tcaagctgat
      241 attggaaaag ttggatttgg ttctatttac aatgagaata aacaatctgt agaaaaggca
      301 atagaggtag cgttacaggc atttgaagat ggaatctaca aagtgcttat aaatgaagat
      361 gttattgaaa aactcgattc ccctataaat cttaaaagtg gagatatatt tacttttata
      421 agacttacct tgcttgctgg aagaatgttt taaaataaat aatttggagg tacataaatg
      481 ttagagcgta ctagagtaga aaaaaatcct aaaataattg aaaattttaa aaataatttt
      541 actaaatctt cttcaaaaca gtactacgac ttagcaaaat ctctttggga ctgcatagaa
      601 aatagttcat actattatag agaacctgaa gacaaattct taaatatgat aaagaaatac
      661 tacaattgcc actactacga aattaaaaat atggacgagc tcctttctgg acctttaaaa
      721 aatgcattaa aattaatatt tacttctgaa caagtagaag aactttgcca attaattaaa
      781 agaaagatag attatacata ttcaggagga tattatcgta aagggtatcg ctcaaataaa
      841 cttgtggatt attttagagg tattataagg tttatttcaa gctatgttga tttaatttat
      901 tatgattttt cagttataga ttatcttact cttccaaata aggtcttgta ctatgactct
      961 ataatgagtg ataaaatagc actagaaata gacaaaggaa atcaaaaagt aataagtgcc
     1021 ttagaagacg taatatttgg agaaaatcaa actgctctac ttacaagaca aatgataatt
     1081 ggtatcatga agagtcatag tgataaatgc tatgaaatgc tttctaaact tcttttggca
     1141 gccaaacaac aagaaggact tagacagcaa attgtagaga gtttagatga gggtacaatt
     1201 tctgcatcaa tatacatgtt aaaaactatt ttggacaaca atttagagcg atttagttct
     1261 gttataagag ccttggatac atggacagga cttggttttt cagaccaaaa acctggaata
     1321 atacgtaaat gtttagaact ttcatataaa tcattgactg atgaaaagtt ccgtatagaa
     1381 tcaattaata gcaatgataa tcttgaaatt tattttgcct tatgggctac tgcaacttac
     1441 aacatatatg actcttactc tcttattgaa aacttacttt gttgccagga aaaatataaa
     1501 aaacttgtag ctctttactt tttatcgcat acagaaagta catattttaa acattctata
     1561 gcatctaaaa acattaaggg tgatgattta gagatactag catgggtaat tacaaatttc
     1621 tatgtagaag gttctgttgg ctactactat agttggcaaa acagagaaac taattttgac
     1681 ttgtcaaaca ctcctattga caaagacttt aatgctagaa aagaacaatt tgaaagttta
     1741 aagcatactt tagaaatttt aaattcaaag acaaaatcat tttcaggaag tgtatttcca
     1801 tggacttgga ttgaaatttc ttctcatgaa atacttaaat gtatgatgac tttaacagca
     1861 tacgatatga atgctcaatt tatagatgtg cttatggaaa attgtcatct tatgagttca
     1921 gatttaagag cttcacttat agtcaatttt gtaaatgaac caaagacaga tagacaacgt
     1981 gaatttttat tggaatgttt aagtgataag agtgtttcca atagagaaac ggtattgagt
     2041 ctacttagaa agcttgaact gacagatgta gaattagata agataaccaa tctattgtct
     2101 ttaaagacaa gctctattag acaaacagta ataaaaattt tacttgaaca agatgattta
     2161 aatttggaaa atattgcttc ttcactcata aaatcttcaa attctcaaaa atgccttgga
     2221 ggtcttgaaa ttgcaaactt attaaaagac aatactgata gacaagaact atataataag
     2281 cttattttac ttatagaaga gattgataaa tctaaaaaga atacatctca agttcaaata
     2341 ctaattgata atttgttagg taaagcaaaa aatgattact gcaaagaaaa tggttttgga
     2401 atatataacc ctgaaaacaa agttcaaatt ccagaacaat ttgattttaa agatgattct
     2461 catagctcat caaagcttaa taaaattatt tcaaacataa gtttatctac tcgcgaggat
     2521 tctgtttctg tagcaacaaa aaaagaagca aaaaaaattc tcgatacgga tataaataaa
     2581 ataataaaag tttatgaaaa atttgatgag cttattactg aaaatgcaaa ttatgagtat
     2641 gaagttgaaa gatggtcagg taatgtagac aaagtacttt taggttattc tacttataat
     2701 ttgactccta ttaaatataa agtttctaat gatactcttg ataattatcc attggcagaa
     2761 ctttggagac aaactagcaa aaaacttgac ttagattaca aattaatact tgatatctta
     2821 ttttatagta taagtacatg tggatataat gaaacagaaa aaagttggtt ttcatctata
     2881 tattcaaatg tccttgactc taaaaaacaa aataaaatat atacaaaact tgaaaaattt
     2941 aaatttaatg gacttacaag aaaactttta agagttttac taaatgagtt tgaccataaa
     3001 gaaatattta actattgttt ggaaatatac tataaactta gaagcttatt aactatagaa
     3061 caatgtaaaa aacaaagagt tgaatttact aaaaactatt acagacaaaa tgaatactgc
     3121 cctgctttaa atgcccctat tttaaactat tggcttgata aagcacattc gtttatttat
     3181 gatgatattt cttttactaa gtttttctac actacgtttt caaattatca gttgctagaa
     3241 tataagtttc ctttctatac tagcataaat gtgtttgctc gtgcacatga actaggatta
     3301 atgactgaag agcaagttaa tagagaaatg ataagtggat acaacgcttc aaatataata
     3361 agaacattaa caaatcctag atgggctggt gacacattag aaaagtaccc ttggcttaag
     3421 gataatttaa aaactgttac aaaccgaatt atagaaattg aatcacgtag aggtgatatg
     3481 ccaacagaag tgtctcatct agctaataat atacaatact ttgaaggtat aaattatttt
     3541 gtaaatattt tggtcgctct tggtaaagaa actttccaga gaggatattc atatggtagc
     3601 aattattcaa aaaaagatgt tttaagttct ctacttaaaa attgctatcc tactaaagaa
     3661 gatgattcaa aaaaattaaa aagtttactt aaaaataccg acattactga gttaagattg
     3721 atagaatctg ctatgtatgc acctcaatgg gtaaacataa ttgaaaaata tttaaactgg
     3781 aagggtctga aaagtacaat ttggtacttt cacgcacata taaatgaagg attctctgca
     3841 caaaaagaat cagaagttgc tctattctcc cctatttcac cacgtagttt cattgatggt
     3901 gcatttgatg tagattggtt tttagattca tataaaacta ttggagaaga aagattcaat
     3961 atactttaca aatcagctaa atatataact agtggtggta actctcatag acgtgcacaa
     4021 ctctatgctg atgctacact tggtaagctt gataaagaca tgttgatgaa agaaattgaa
     4081 acaaatagaa atcaagataa attgagaagt ttctctttac ttcctatgaa aaaaggagac
     4141 ctaaaagaag caaccttccg ttatgaattt attcaaaagt ttgctaaaga aagcaagcaa
     4201 tttggtgcac aacgtcgtga aagtgaatca aaagcttgta ctatagcact agaaaatcta
     4261 gctagaagtg ctggatttgg agatgtaaat cgtatgatgt gggctcttga gactgaaaaa
     4321 ctaaatgaag ttgctcatct atttaaacca aaagatattg atggcacttc aatttggata
     4381 gatgttgatg aaaacggaaa agcgtctact aaagttcaaa aaggcaataa gtctttaaag
     4441 agtataccaa aatctattgc taaacatcca tatgtacttg aacttaaatc tgttcaaaaa
     4501 gatttaaaga gtcagtattc tcgtgcaaaa cagagtttag agcaagctat gaaaaatgaa
     4561 atagtattta cagttaaaga aattgccaat atctataaaa atccagttat ctgccctttg
     4621 attaaaaatc tagtatgggt atgtgatgat aatattggat atgtttataa tgataagtct
     4681 aagttcttta ttttcaatat agaaggtgaa acttataagt tgacagcaaa aaataaatta
     4741 aaacttgcac atccagttca tttgtttgag tctggtaagt ggagtgagtt ccagcatgat
     4801 ttatttagta agcaaattgt tcaaccattt aagcaagtat tccgtgagta ttatagaata
     4861 aacagtgatg aattatctga aaaaactata tctcgcagat atgctggtca ccaaattcag
     4921 ccaaagaaaa ctgtagcact tcttagaaat caactttgga ctgtcgatta tgaagaggga
     4981 ttacagaaag tattttataa agaaaatata gtggcaaaga tgtttgcact tgcagattgg
     5041 ttttcaccag cagatataga gcctcctact attgaaacag tagaattttt tgatagaaaa
     5101 acttataaaa gcttagaatt agcttctata cctccaattc tattctcaga agttatgcgt
     5161 gatatagacc ttgttgtaag tgtcgctcat gctggtggtg ttgaccctga aactagccat
     5221 tctactgtag agatgcgtat agcaattgct aaagaacttt tattattatt aaaaattaac
     5281 aatgtcaatt ttgaaggctc tcatgctctt atagcaggta atttaggtga gtattctgtg
     5341 catatgggtt ctggtattgt acataaaaaa gggatgggtg ctttgaatat acttcctata
     5401 cattctcaaa gtagaggtaa gatattcctt ccattcgctg atgaagaccc taaaactgct
     5461 gagattatgt ctaagatact attacttgct gaagatagta agattaaaga ccctagtatc
     5521 ttaagtaatt tataagtata aaaactatcc ctaaaatata ttggactttc ttaaaaaaaa
     5581 ttagaaagtc caatatatac tatactgtaa tttatataga ttaaatcata attacaaata
     5641 atttttatat aaacttctct tatacattat ctttatggta cttattatat cacacaaaaa
     5701 gtatttttct tctatcttca ctagaatctt ttaaccctat aaaatctata taaaaaatct
     5761 ttttctagag atttgtttaa tatggcattt tcttgaaaat ttataaacct tatctctaat
     5821 gaagatttat ttataggtat aaccttatgt ataaatctta gatttactat gtattgtcta
     5881 tgggtcataa agaaattagg aggtaggcgt ttttttaaat catcaaagtt ctcataaaac
     5941 tttattatat cttcatcttt agtgtgtaga ttaattgctc ttgaattttt ctcaaacata
     6001 actatatcat caaaattaat catatgtatc tgcttcctta ttttatatac aaaaagggtg
     6061 tcttctataa aattattatt agcaatctta tgggagttaa tatggtctat tgctatgtta
     6121 attgaatcaa gtattttttc ttttttaaat ggtttaacta caaaatctat aggatgcact
     6181 ttaaagcttt caagactata atcagcgaaa cccgtcacaa atactatatg tatatcctta
     6241 tctatagaaa gaatttcttc agcagaatca atcccatttt tatatggcat atcaatgtca
     6301 ataatcaata aatctatttt ctcatttttt ataaattcaa ttgcctctat accatcctta
     6361 acttttttaa cataatctac atatccagac tctttagcta tatttgctat atcatctctt
     6421 acaatcttat catcatcaca aatcaaaact tttatcatag acttttctcc tcctaactaa
     6481 taatattttg ggattaagat tacaaattca gttaaaaaat catcactatt tacaagtata
     6541 ctataatcat acttatctaa tatattttta actaaatgta gcccatatcc cctatcttgt
     6601 ccttctttag tactaaatcc attccaaaat atactttcaa cattatgtat tttaggacca
     6661 ttatttttta tagctataat atactgaaat tcatcttcat atgcatgtac tcttatatac
     6721 ttcttttctt tttttatagt agacaaagac tctattgcat tatctattat gtttaatagt
     6781 actgaactca tatcattagg ttcaatctct aaattatcca taaaatcttg aatgtagaca
     6841 tcaaaagata tatccttttc aagacattcc tcgtatttta atgaagctat agcatctaaa
     6901 taagatactt ttccatacct tgaatttttt gatttaaaac tatttgatat attcattata
     6961 tattctttgg catcttcatc tagcccagta tttaatagag aatacaccac ttgcaaatgt
     7021 ttcaaatagt catgtttttg acttcttaat ttgataatta tattatcctt atgatttatt
     7081 ttactatcta gtttttgtag tttatattcc tgttcattat tattatttat caatcttata
     7141 actttaaagc ctgtataaaa acttaaaata attaaaacca aaaaaagtat aatatttaat
     7201 actccttcaa gtaaaaaact atatcctaac cttaattgta tatatttagt gccctccata
     7261 aataaaatca atgcaaaaca cgatataaaa aatgttaata tgcctctttt taatatgtct
     7321 aattctttgt aaaatttata cttattcatt ataagattgc taaatataaa caaagttagt
     7381 attactatag gaaatacaaa attgttagcc atgatttccc tcccctctac aatttatata
     7441 gggattatat cattttaaaa aaattatgta aaaagttaaa atttacatat agattaatta
     7501 aacttgtaaa aatacagatg aatttgtaca attaactact taaaacttga aaatacttac
     7561 tttgctatat attttagata aatatataat tgatttagtt aagaaaggaa gtggtaaata
     7621 tgaaagataa tttgaaaata gtactaaggt atataaaaag ttataaagct aggtctttag
     7681 caattgttgt gagtataatt cttgctacat cactcgttgt tggtattggt accctctcaa
     7741 gaagtgctca gcaagcagat gtagatagat tgaaaagaga gcttggaact gaccatgtat
     7801 actttaaaaa tataaagaaa aatcaattaa agtccataaa atctagcaaa gatattaaaa
     7861 atctgggtat aacatcttac tatgcgtcta cagatgttaa tgaaaaatta cctataaaca
     7921 tactatatgc aaataaaaat tatttgacta gtcattctaa acttataaaa ggtcgatttc
     7981 caaaagctag caatgaggtg gtagtcgaga aatggatttt aaatagtatg ggcttaaaac
     8041 cagagataaa tcaaaacctt acttttaaat tatatcaaaa agaaaatcca gaaactttta
     8101 aagttgttgg aatccttgaa gatagattta tagagaaaaa aaagggacaa tgtgaaattt
     8161 tttctaatct caatgaatca gaactgaatg attttacaac ttatattgaa tttaatgaag
     8221 gcagtgatat aaatacaaac atagacaata taataaaaaa tgctatgtta gatgagaaaa
     8281 gtataggaag aaactcaatg cttatagaat ctactatgaa aaacggtact ttagataatt
     8341 caagtaaata cgttgcaatt gctatgagtc tatttgctgg tattgtaata tatagtatct
     8401 atgttatatc aatataccaa agagtacagg aatatggcgt cttaagagct attggttcca
     8461 ccaactttag gattttcaaa cttatgcttt atgaactttt catacttgct ctaatagcaa
     8521 tgcctatagg tatatgtata ggtatgggag gtgcacaaat atttaatagg tctgtaggaa
     8581 atattcaatt tgaaggaaat ataaatgtta caccttttgt tattccagat aaaataattc
     8641 tcttatcaat tgcatgtaca atactaataa tactaattat aagtttcttt acatacttaa
     8701 aaataagaag aatttctcct attgatgcta tcagaaaaac ttttggagct gataaaaatg
     8761 taaccaaagt taattattta atatcaaaat taactttaaa tatgtcagta acaaaatcta
     8821 tatctgcgaa aaacatcttt agaaataaaa aggggttcat tataattgta ttatctatga
     8881 gtataggagg tataatcgtc ataaaagaca attataaata ttctttttta gatgtgcaaa
     8941 acaaatctac tcaagaacaa acatatatga atgcagattt tattctatca aatgtttatc
     9001 tcaatgaaat tggtaacaat acttctttta aagatataaa agggtttaat gataatcaga
     9061 tagataaaat aaaaaatata gatggaattg acaaagtcaa aactgctagt atcttaaaca
     9121 caagaataga ggtaaataaa ataaatagat tagattacta cgaagctata aattcaactc
     9181 cttattataa gctaaatcct ctgttcataa aaaataagac tactggcaaa tatacactaa
     9241 agcaaaagct aaaaggttat aatgatgaga tgataaagtc attagaaaaa tacttagtaa
     9301 gtggaaatat aaacttgaaa aaaatgaaaa aagaaaattt agctattgta tatgtaccag
     9361 aaatttctaa aacacagaaa tacacaaaga cttttactcc tggtgaagga acaccagctg
     9421 tagatataaa agtaggtgat actataaaag tcaaatatcc aaagggtggg atggatacag
     9481 ggctttatga aagtctaaaa gataattatg aatacttaga atatgaattt aaagttggag
     9541 ctattataag ttatcctttt gcagataatg gtatgtactc tggagatgat ggtgtagatg
     9601 taataacgag tagcgaatac ctaaaaaaat taactggtgt agataagtat aacttagttt
     9661 atgcagacct taatgaaaat gcaaacaaga aaaaaataaa taacctttta ggtaaaattg
     9721 gaagtgaagt tcctggaact acaacagtag atatgatggt agaaaaagaa aattctgata
     9781 agatgacatc tagaactatg atttatagtt atggtatagt agcaataatg ttcataatca
     9841 gtgtacttaa cataataaac aacataagtt ataacttaac ttcaagaact agtgaatttg
     9901 gaatgttaag agctattggt atctctgaga gaggctttaa aaatatgata ctttatgaag
     9961 gaatattata tggtgtactc tcaagtataa ttactatcgt gtcaggaatt ataatacaat
    10021 ttaaaatgta ttatatgcag ggctttgtat catatggatt gggattttca atagattaca
    10081 aaatatatat cctagttatt ttagcaaata taattgttgg tattctagct acatatattc
    10141 catcaagaaa gataaataaa attagtatag ttgaagctat aaacattaca gaataggagg
    10201 gataattatg cctatattgg aaactattaa tttaggtaag atttatggga aaaaggaaac
    10261 atctgtacat gcactaaaga atgcaaattt aaaaataaat aaaggcgaat ttgttgctat
    10321 aattggacca agtggaagtg ggaaaagtac atttctacat ttagtaggtg gtcttgaaag
    10381 accaagtaat ggtactataa aggtagatgg taaagatata tgttgtcttt cagataaaga
    10441 attagcaaga tatagaagac aaaaggtagg atttgtcttt caacaatata acttaattcc
    10501 tgtattaaat gtaaaggaaa atatagaatt accactaaag ttagacaata aaaaaataga
    10561 taaagaatat attgatgacc ttataaattt attgggtctt aatgaaagga aaaatcatct
    10621 tcccaaccaa ttatctggtg gtcaacagca aagagttgct attgcacgtg ctttatctgc
    10681 aaaaccaagt ataatcctag cagatgaacc aacaggaaat ttggacagta aaactacaga
    10741 agaagtcatg gatttactaa aaagttctat aaaaaaatat aaccaaacac ttataataat
    10801 aacacataat gaaaatattg ctagaaaagc agatagaatt atatctataa tagatggaga
    10861 gcttaaatta acactctaaa actatttata gaaaagagga tttaaatgtt attgatttta
    10921 ggtaatttaa atttaaactt ggattttgac tacagaataa taagagaaga aaatgaagat
    10981 gtagatatat ttatagatat taattatcgt agcttagata tagatactga tggttctaac
    11041 ttatttaatt ctagaataca atttccattt gtaagagcat tgattctaag gctaaataag
    11101 aacaatcaat gtatgacaat tcatttgtta agagatatag atttattctc tgcttttgcc
    11161 aactttgaag tggattatac tgactctata ataaatataa aaaatcaaaa tgagaaagta
    11221 attttaaata aatctatcaa aaaatagtaa gggggcctta ataggcc
//
LOCUS       DAELJO010000129        11252 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELJO010000129 DAELJO010000000
VERSION     DAELJO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.129"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            327..1073
                     /locus_tag="KPB88_002682"
     CDS             327..1073
                     /locus_tag="KPB88_002682"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG0054950.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            1103..2038
                     /gene="rsmH"
                     /locus_tag="KPB88_002683"
     CDS             1103..2038
                     /gene="rsmH"
                     /locus_tag="KPB88_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG0054951.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            2519..2866
                     /locus_tag="KPB88_002684"
     CDS             2519..2866
                     /locus_tag="KPB88_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054952.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            2878..4857
                     /locus_tag="KPB88_002685"
     CDS             2878..4857
                     /locus_tag="KPB88_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG0054953.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            5024..6397
                     /locus_tag="KPB88_002686"
     CDS             5024..6397
                     /locus_tag="KPB88_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG0054954.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            6438..7406
                     /locus_tag="KPB88_002687"
     CDS             6438..7406
                     /locus_tag="KPB88_002687"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG0054955.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            7425..8780
                     /locus_tag="KPB88_002688"
     CDS             7425..8780
                     /locus_tag="KPB88_002688"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG0054956.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            8832..9962
                     /gene="spoVE"
                     /locus_tag="KPB88_002689"
     CDS             8832..9962
                     /gene="spoVE"
                     /locus_tag="KPB88_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG0054957.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            9980..11206
                     /gene="murG"
                     /locus_tag="KPB88_002690"
     CDS             9980..11206
                     /gene="murG"
                     /locus_tag="KPB88_002690"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0054958.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         4468 a         1207 c         2172 g         3405 t
ORIGIN      
        1 aacaaattta taaaatatat ttatataaat tgaggtaaaa atttaagagc tatagataag
       61 aattagaaga ggttgatgta tatatgcatc aatctttttg attaaaaaat atctatgtac
      121 acttgtaact ttagtcatga gagattcagt ttaaaaaatt ttatagtaat tagatataat
      181 atagtgtagg gagttgtaga ataaatttta atattttttg tagaattaac attaatgtgt
      241 tatcataatt tagtataatt acaattaatt gtaattatag catattgctt tggaaatatt
      301 ttcaattaat acaaagaggt gacgttatgg atagagtagc atttacactg tttggcatag
      361 acatcatgtg gtatggaata cttatggcat gtggaatgat tttaggaact ttaatagcaa
      421 taaaagaagc aaaaagagtg ggaattaaag aagatgatgt attaaatata gctataatag
      481 caattccagt tggactaatc tgtgcaagaa tctattatgt tgtatttaat tggagttact
      541 atgctcaaaa tatgtctcaa atttttaatt ttagaggtgg tggacttgct atacatggag
      601 ggttaattgg tggaatatta gctggttata tatacactaa gattaaaaat attaacttct
      661 taaaaatggc tgatacagtt atacttggaa tgcctttagc acaagctatt ggaagatggg
      721 gaaattttat taatggagaa gcacatggag gtgctaccaa tttaccttgg ggaataatgg
      781 ttgatggagt aaaggtacat ccaacttttt tatatgagtc tatatgggat tttggaatat
      841 ttatagtttt attactattt agaaaaaata aaaaatatga aggtcaagtt atagttactt
      901 atataacttt atattcaata ggaagatttt ttatagaggg attaagaaca gatagtttaa
      961 tgttaggacc acttagaatg gcacaagtta taagtctaat tggagttatt ggtggcataa
     1021 ttgcacatgt ttatttatca aagaaaaata aaaataatat tagtgaagaa tagtttaaaa
     1081 agtgaattta agggaggtaa ctatggaatt tcatcatgta tcagttcttt taaatgagtg
     1141 tatagagaat ttaaatataa agccagatgg cgtgtatgtt gactgtacta tgggaggagc
     1201 aggacattct aaagaaatag taaaaaaact gtcagataag ggattattta tagggtttga
     1261 ccaagataaa aatgctataa gcacagcaaa agagagattg agtgaatatg aaagtagagt
     1321 gaagtttgtt cacagcaatt ttgaaaatat aaaagaggaa ttagaaaaaa taggagttta
     1381 caaaattgat ggtgtattag ctgatttagg agtttcatct catcaacttg atgaagctga
     1441 tagaggattt tcttatatgc aagatgcacc acttgatatg agaatggatg ttaggtgtga
     1501 gttttctgca tatgatgttg taaatactta tacagaagat gaattaacta agataataaa
     1561 agattatgga gaagataact gggctaagcg tatagctaaa tttatagttg aagaaagagc
     1621 aaacaaacca atagaaacta caggagaatt agtagatgta attaaaaaag ctatccctaa
     1681 gaaagctaga atagatggac ctcacccagc taaaagaact tttcaggcaa tacgaataga
     1741 agtaaataat gaacttggag taattacaaa gatgataaat gatgcttctt ctataatgaa
     1801 tgaaggtgga agaatatgta taataacatt ccattcatta gaagatagaa tagtgaaaaa
     1861 tgcattcaaa catttagcat cagattgtat atgtccacaa catttaccta tatgccaatg
     1921 tgataaagaa tctgaggtga aaataataac tagaaaacct atattaccta gtgaggaaga
     1981 aatagaagta aatccacgtt caagaagtgc taagctaaga gtagcagaaa aaatataagt
     2041 agttataagg ctaaagaata atcttatata taagattatt ataattaatt aaaaagtatt
     2101 atatattttg ttgcactggt aaagcaatta aaaaacaatt aacactataa gaaatgttct
     2161 ttgttattga gatatataat gaaaatttag atataaaaat atagatatag atggtattaa
     2221 gtaatataat ggagaactta ggaatcagag agtagatagt tagttacata ttatatttat
     2281 ggaagaaatt atctaaaaat actagaagtg ataaaaataa atagaaatta atatctaacc
     2341 tatagattca tataaaagta taagtgaatt tataggtttt tgttgtgtat agaaaaaaat
     2401 acaattagat tgtgtaatag tgtattaatt tagcgatgaa attttagagg aagaaatatg
     2461 attaataaca aatatagtat agaggaaaat ttaaactata tactatcggg gtgagaattt
     2521 gaaaaaaaat tataaaatac ataatctaaa tgaatataaa aataaaaaaa agaataatta
     2581 taaaaagaga aataagaaaa aaataggagc taaatgcatt ttactgattg caactttttc
     2641 agtaataata ttgaatctat gtggttattc aaaaatctcg gaaatgaagt acgatatcta
     2701 ttatttacaa aaagatttaa gaaaaaaaga gatagtatta gaggaactaa aggcaaaaga
     2761 aactgaaaaa acatcaataa aagaaattga gctaaaggct aaggaattgc tcaatatgga
     2821 ctatccaaaa gatggacaaa taaagtatat agatgtggat aactaggggg aatagatttg
     2881 agaaaagtaa agaggataag taagaaaagg ctggtactcg ttcttattct agcatgtgca
     2941 ttgttttttt gccttgttat taggactggg tatctacaac ttatgaaggg aaattggttg
     3001 agtacaaaag cactagaaca acaaacaaga gatataccaa tagaacccaa aagaggaact
     3061 atatatgata gaaatatgaa agagttagca gtaagtgtaa ctaagtatac tgtttggtgt
     3121 aaacctgtgg aagtagaaga taaaaaagaa gcagccgaaa aggtagctga gattttggat
     3181 gaagattata aagatattta cgctttaata agcaaaaaaa atatggctct tgtaaaagta
     3241 aaaagatgga tagatgatga taaggcaagt caaataagag atgctaaatt aagtggaata
     3301 tgggtggcag aagataatca aagatattac ccatatggga attttgcacc atatgtgtta
     3361 ggacatactt cttcagatgc aacaggtata tctggtgtag aaatgcaata tgataaaaag
     3421 ttaaaaggaa agcctggaaa acttatagta agtactgatg cttcaggaag agaaatacca
     3481 caaggaatgg aaaaatatta tgagcctgta caaggtaatg gtcttgtgtt gtctatagat
     3541 gaagtaattc aacactatac tgaaaaagca gtacagaaag cttatgagtt aaataatgca
     3601 aaaaaagtta ctgcaatagc tatgaaccct aaaacaggtg atatacttgc tttagcatca
     3661 aaaccagatt atgaccctaa tgactcaaga acgccaatat atccatatta tcaagaagaa
     3721 cttgaaaaat ataatgataa agataaaata aaaggctatt atcaaatgtg gagaaatcca
     3781 gcagtaagtg atacatatga accaggttct acatttaagc ttattacatc ttccagtgct
     3841 cttgaagaag gagtaattaa agatggagaa aaattcacat gtacaggtag tgtaactgtt
     3901 ggaggaagaa agattaaatg ttggagacat tacagacctc atggaacaca agaatttaaa
     3961 caagcagtac aaaactcttg caacccagta tttgttgagt taggaagcag attaggtgtt
     4021 ggaaaaatgt atgattatat agaatcattt ggtcttatgg ataagacagg tatagattta
     4081 ccaggagaag ctaagggaat actgtataac gaaaaaaatg taggaccagt tgaattggct
     4141 acaatatcat ttggtcaatc tatatcagta actccaatac aacttataac agctatatca
     4201 tcaatagcta atggtggaga tttaatgcaa ccaagagtgg taaaatctta tactgacaat
     4261 aaaggaaata taactgaaac agtaaaacca aaaaaagtaa gaagtgttat atctaaagaa
     4321 acatctaaaa aaatgttaga aattgcagaa tcagttgtaa ctgaaggtgg aggaaagata
     4381 gcatatatac caggttatag acttggtggt aaaacaggta cagctcaaaa agttatagat
     4441 ggtaagtatg ctccaggaaa gtatatatgt tcatttgttg gaatagcacc ttgtgatgac
     4501 ccgcaaatag tagttcttgc tattgttgac gaaccaacag gagtaagtgc atttggtagt
     4561 actacagcag gaccaatagt gaaagaaata atgaatgatt ctttaaaata tttgggagtt
     4621 aaaccagtat ataaagaaga agaaaaagct gagtatgaaa agaagcaagt taaagtgcca
     4681 gatgtaagaa atctaaaaat aggagatgct gtaaaggcat tggaggatgc gaagttaaaa
     4741 ccagacttag atgcagatat agaacttcca gaagatacaa aggtaaagga tatattccca
     4801 aaacctggag taaaagttaa tgaagactca agtataaccc tatattttga aaactaaaaa
     4861 gtaacagata attaacatat acccataaac atcttcaaaa aaacagaaat atagtatata
     4921 attataaagt taagagttat cgtaggcaat gtatatataa cggtatacat tgccttttgt
     4981 tgctaagaga atatagctaa ggttttaaag gaggagttta ataatggagt gcttaacaat
     5041 aaaagagtta gtacttgcaa ctaatgggaa gttaatatat ggagattata atgactgtgt
     5101 aagtgatata gttatagata gcagggaggc aaatgcccaa aatgcatttg tagccatagt
     5161 tggagaaaat ttagatgggc atacatttat gaagtctgca tatgatagtg gatgtaaaac
     5221 ttttataaaa aatgaaagta atggaataaa attagaaagt tcggatataa atttaataga
     5281 ggtaaaagat acatctttgg ctctaggaga tatttcaaaa tactataaag aaaaatttga
     5341 tattccattt ataggtgtta caggtagtgt gggaaaaact actactagag atatgattta
     5401 ctctgccata tctgcaaagt taaatatact aaaaaatgaa aaaaacttaa ataatcattt
     5461 tggagttcca ttgactttat ttaatctaaa taaagaacat gagtgtgctg taatagagat
     5521 gggtatgtct ggattcaatg aaataaaata tttagtggat atagtaaatc ctaaaattgc
     5581 agtcatatcg aatataggat tatcacatgt agaaaaacta ggttcacaag agggtatttt
     5641 gaaagcaaag atggagatta cttcaaactt tgatgaaaca aatactttaa ttgtaaatgg
     5701 agatgataag tttttatcta cattaaaaga aaaagaacat gcatataagt taaaaacatt
     5761 tggctttaat aaaaacaatg atgtttattg tgaaagttat actatggaag aggatagttt
     5821 aacatttata tgtgtaataa atgggaaaaa agaggaaata tttataccaa caataggaga
     5881 acataatata tataatgcaa tgtctgctat attagttggt ttgtgtctaa atatttcttt
     5941 agattatata aaaaaaggtt taaaaaattt taaagggaca aaaatgagac tagacataat
     6001 aaaaaatgaa aaattaacaa taattaatga ttcatataat gctagtccag attctatgga
     6061 tgctgcttta aagatacttg gaagatataa aggtagaagg gtagcgatac ttggagatat
     6121 actagaaatg ggtgaaattt cagagtatgg tcatagatta gttggaaaat catctatgaa
     6181 taatactgat attataataa ctataggtga aaattcagtg tttattggag aagaagcaaa
     6241 acaattagga ttcaattcag ccaatatata tcactttgag aatagagaag atgtgtttaa
     6301 caagttaaat gaattagtaa aaacaggaga cactatttta gtaaaaggct caagaggcat
     6361 gagattagaa aaaatagtcg aatatctaaa taaataagct taaactaatg agttaattga
     6421 caagggagag gagttttatg atgttaggaa taacagagct tacgtacacc gcattgattg
     6481 cgtttttaat agtaattata ataggaccaa tatttatacc aatgcttaga aaatttaaat
     6541 ttggtcagac agttagagat gatggacccc aaacacatct agctaaaaac ggaacaccta
     6601 ctatgggtgg aattataatg attgttgcaa ttctaataac tggacttaca agagtaaaag
     6661 ttagtcatga tatggcagta ggtcttatat gtattgcagg ttttggtttt attggctttt
     6721 tagatgattt tataaaaata aagctaaaaa gatcacttgg actaaaagca tatcaaaaaa
     6781 taatccttca agttgcatta tctttttatg ttgcttttta tcagtataca tcatcatcaa
     6841 gtgcttcaca acttatgata ccatttacag attttgtgat aaatgttgga atattatata
     6901 taccaataat gatgtttata atcgtagcaa tagtaaatgc tgtaaacttg actgatggat
     6961 tagatggatt agcatcagga gtaacattaa tagtttctgt attctttatg ctatttgcaa
     7021 gttctattgc tggaaataca gaagtcgcag ttttagcagc agcaactgtt ggagcatgtt
     7081 taggtttctt aggatttaat tcatatccgg ctagagtgtt tatgggcgac acaggttcaa
     7141 tggcattagg aggtgcagtt gtagcatttt cagttctaac taactcagtg cttataatac
     7201 ctataattgg aggaatttat tttgcggagg ctctatcagt tttgattcaa gttggatatt
     7261 ttaaggcaac aagaaagaga ttctttaaga tggctccaat acaccaccac tttgaacagt
     7321 gtggttggcc agaaacaaga gttgtattta ttttttggat tataacagtt gttttagctt
     7381 ggataagtat aatagcagta ttttagggct agggggaatg aaaaatggac ttaaaaggaa
     7441 aaaaagtttt attagtagga cttgcaaaaa ctggtatatc tacaattaaa catttagata
     7501 aattgggtgc aagtataatc gtaaatgata taaaagatga aaataaattg agaaatatac
     7561 ttgatgaatt gaaatctata aatgacataa aatacatatt aggacatcac ccagaagatg
     7621 tagatgatat agatatggtt gtagtgtctc caggtgtacc actagaccta ccattcatat
     7681 tgaaattaaa aaattcaggc aagtatataa ttggtgaggt agaattagct tttaaactat
     7741 cgaataatcc aatttttata gggattacag gtacaaatgg aaagacaact acaactagtt
     7801 tggtaggaga aattttttca agagcaaaac gagatacata tgtagttggt aatataggaa
     7861 atcctgtaat agatactata gaaacttcaa gtgaagagtc tgtgttagta actgaactaa
     7921 gcagttttca gcttgagagt atagatgaat ttagacctaa agtaagcgca atattaaata
     7981 ttacagagga ccacttaaat agacaccata ctatggaaaa atacatagaa gcaaaggcaa
     8041 atatatttat gaaccaaact gttgaggatt tctgtgtatt aaactatgat gatgagatag
     8101 taaaatcttt agctgataaa tgcaatgcca aagtaattta cttttcaaga actaaaaaag
     8161 taaatggagg agtatattta gaaaataatg acataataat agatatagat gataaaatca
     8221 aatttctaaa taaagatgat gtaagtttac ctggaggtca taacttagaa aattgtatgg
     8281 cagctatagc aatagcatat gtctgcaaaa ttgatttaga agttataaag gatgtactta
     8341 tgacatttaa aggagtggaa cacagacaag agtttgtaag aaatttagac aatgtaatat
     8401 atgtaaatga ttcaaaggga actaatccag attccactat aaaagctata cagtcttatg
     8461 atagacctat aatacttata gcagggggga tggataaggg aagtaatttt gatgaacttc
     8521 ttgaaactgc aaagtcatat gttaaatcat tggtcttatt aggagaaact gcatcaaata
     8581 tagaaaattg tgctaaaaat aaaggattta atgacataca tatagtaaaa gatatggaag
     8641 aagcagttaa gacttcttat gaaatttcaa aaagtggaga tatagtgctt ctttcaccag
     8701 cttgtgcaag ttgggatatg tatgaaagtt ttgaagtaag aggaaaagac ttcaaagata
     8761 atgtaaacaa tttaaaataa tctcttacct ctaaacttac ttaaatggaa gaatggaggt
     8821 agattagttg gatgcctaaa gaaaatttga aaaaacaaat tgacataagg atgaagactg
     8881 aatttgatgg agtagtgttt tacactacta tgcttttagt atttgtagga atagttatgg
     8941 tgtttagtgc aagttttata caatcttcat ttaaacacaa tgatgcgtac tattttctaa
     9001 aaaagaatgt aatatatgct gtacttggat ttatagttat gattattaca tcaagaatag
     9061 attatagttt ttggaagaaa aatgctacag caattggagc tatagcagta gtattattat
     9121 tgttagtttt gaccccttta gggatagaag caaacggagc aaaaagatgg ttgggaatag
     9181 gagctttaac atttcaacct gcagaaatcg caaagtttgc tacaataata ttaactgcaa
     9241 aattaattga aaaaaattat gataaaataa aatctttgac aaaaggtgtt gtaccacttc
     9301 tcgtagttcc agggatattt tttgcgttga taatacttca gccaaatttg tcaacagcag
     9361 gtactgtaat tctagttact tttgtaatga tatttgttgc tggaatggat atgaaaattg
     9421 tatttgctat gatagggagt ggggctgctt tatttgctgc attagtaatt gctgaaccct
     9481 atagacttag tcgtgttaca tcatttttag acccttttca agaccctctt ggtaaggggt
     9541 atcaggtaat acaaggtcta tatgcactag gttcaggtgg attgtttggt ctaggcttag
     9601 gaaagagtaa acaaaagtat ttttatatac cagagccaca aaatgacttt atatttgcta
     9661 taataggaga agagttagga ttgattggtt gtataatagt tattatgcta tttgtagttt
     9721 tagtctacag atgtgtaaga atagctttga agacttctaa tgtatttgct tgtatggttg
     9781 ttataggtat aggtgcacaa attggaatac aagcagcttt aaatatagca gttgcgacat
     9841 catcaatgcc agctacaggt gtagcactac catttataag ttatggagga acttcactca
     9901 ccatctttat gggagctgta ggaatagtgt taaacatctc aaagcatgtc aaaattaatt
     9961 aacaggagat gatgatttga tgaaagtttt attatcaggc ggtggaactg gagggcacgt
    10021 gtatccagct atagctattg caaacaaaat aagagatgag catccagacg ctgagataat
    10081 ttttgttggg acggaaaagg gaatagagtc agaaatagtt cctaagtatg gatttgaatt
    10141 aaagactgta acagttcaag gttttaagcg aaaaatagat ttcgataatg taaaaagagt
    10201 ttttaagctt tttaaaggtt tggagcaatc aagaaagatt gttaagaaat ttaaaccaga
    10261 tatagttata ggaactggtg gatatgttag tggacctgtt ttatttaatg catctatggg
    10321 gaaaatacca gccataatac atgagcaaaa ttcttttcct ggtgtaacta ataagatttt
    10381 atctaaaaca gttacaaagg tacttacaag ttttgaagat tcgcataaaa gatttccaga
    10441 ggcagcagaa gataaattag tttttacagg aaatccagtt agaaaagaaa ttttattatc
    10501 aagaaaaaat attgcaagaa agaatctatc aataagtgac gaaaaaagaa tggtactttg
    10561 ctatggtggt agtggaggtt caagaaaaat aaatgatgca atgagacttg taataaagaa
    10621 tatggtaaat gaagatatag cttttatatt tgctacaggc aaaagctatt atgatgaatt
    10681 tatgggaagt attagcgata taaatcttaa accataccaa aaagttgtgc cttacttaga
    10741 agatatggca aatgcacttg cagcaagtga tttggttata ggtagcgcag gagcaatttc
    10801 attagctgag ataacggctc ttggaaagcc atctataata atacctaaag cttatactgc
    10861 tgaaaatcat caagagtata atgccaagag tatagaaaaa caaggggcag gaattgctat
    10921 tttagaaaaa aatctaactc cagagagcct aaatacagca gtatttaaac ttttaggaga
    10981 tagagaactt ttggttgata tggcaaatgc aagtaaaact ataggtaagc ctgaagccat
    11041 agaccttata tatgatgaga taatgaaagt atacaattca acacaaaagt caacttctaa
    11101 aaaaacaaaa aaagaaaaag ttattaagga agtaaaagaa ataaaaaaag aaacgactcc
    11161 aagtatagaa ggtcaagcta aggtaatagg aataaagaaa agataagtaa atatttaccc
    11221 ctaatttata atatattaga ttttctaaat ta
//
LOCUS       DAELJO010000130        11127 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAELJO010000130 DAELJO010000000
VERSION     DAELJO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.130"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            139..1011
                     /locus_tag="KPB88_002691"
     CDS             139..1011
                     /locus_tag="KPB88_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0054959.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            1138..1410
                     /locus_tag="KPB88_002692"
     CDS             1138..1410
                     /locus_tag="KPB88_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054960.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1572..2144)
                     /locus_tag="KPB88_002693"
     CDS             complement(1572..2144)
                     /locus_tag="KPB88_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0054961.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2328..3170
                     /locus_tag="KPB88_002694"
     CDS             2328..3170
                     /locus_tag="KPB88_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0054962.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3273..4349)
                     /locus_tag="KPB88_002695"
     CDS             complement(3273..4349)
                     /locus_tag="KPB88_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0054963.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            4845..6248
                     /locus_tag="KPB88_002696"
     CDS             4845..6248
                     /locus_tag="KPB88_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0054964.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    6339..6579
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6622..7320
                     /locus_tag="KPB88_002697"
     CDS             6622..7320
                     /locus_tag="KPB88_002697"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG0054965.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            7343..8344
                     /gene="trpS"
                     /locus_tag="KPB88_002698"
     CDS             7343..8344
                     /gene="trpS"
                     /locus_tag="KPB88_002698"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG0054966.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(8996..10563)
                     /locus_tag="KPB88_002699"
                     /pseudo
     CDS             complement(8996..10563)
                     /locus_tag="KPB88_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(10538..10681)
                     /locus_tag="KPB88_002700"
     CDS             complement(10538..10681)
                     /locus_tag="KPB88_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054967.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(10899..11081)
                     /locus_tag="KPB88_002701"
     CDS             complement(10899..11081)
                     /locus_tag="KPB88_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054968.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         3987 a         1351 c         1718 g         4071 t
ORIGIN      
        1 tgtgatagaa tattaatgaa taaacgttta agtgaggatg aataatattt ttaggaaact
       61 acttctgata aatttatttg taaatttatc gtgtgttatg cagggtggtt tatttattat
      121 gcaaaggggt gcgttttgat gtatggtgaa agtaaaaagc taagtattga taataatata
      181 tttttacaat attgtcttat tatattgggt atgattctat caaccttagg aataaattta
      241 tatctagcac cagccaagtt attaagtgga ggagtagctg gaatttgtgt tatattatac
      301 aaattatttg gtataaatca gggaatatca agtttcttga tgaacatacc aatatttatt
      361 atagctagaa agtattttga taataaattt ttgctaataa gttttgtcaa tatgttacta
      421 ttttctctag cactggggct aacacaagat attgctaaat atttcccaat agatgatact
      481 atgctacagt gtatttatgg tggtgcatta actggtatag gtatggggtt aacttttaaa
      541 gcaagagcta ctgctggagg acttgatata atagcagcca taatgaaaag aaagtatgat
      601 atacctatga aaaatacatt tttatttatt aatttttttg tagtatgtgc aggagctttt
      661 ttatttggag ctaaattggt gatgtacact ttaattacaa tgtatataat ttcttttact
      721 atggatatag gaaaagattg ctttgataga aaaaaatcta tacttttaat atcaaataaa
      781 tatgatgaaa tatccaaagt gataatgaat aaaatgggta gaggagtcac ttttttggag
      841 gcagaaggtg catatacaca aaataagaaa aagatgattt attgtataat atctgcaaat
      901 gaaataggta aatttaaaga tttaatttat gatatggata gtgaagcttt tatttctgtc
      961 aataacgttg aagaagtaaa aggtggaggt tttaaggata aatttcttta agattatata
     1021 tgttaattat atatgttaaa aaatgccata tgtattgaga ttatacctag ttagatgtat
     1081 actgtagtta atggggtaca tgaagtactc aataatatac taggaggtat aagaattatg
     1141 ttaagagaga gatttgtagt aagaaatcat cataatccac atacaagtgg gtgttgtggg
     1201 actggaggag aaggatgctg tggaggagat aaccatgaac attcacatca acattctcac
     1261 agtcatgggc atgaacatga gcatgtaaat ggtgtaggtc acgaccataa tcatgatgat
     1321 ggtttttcag gtactcatga gcatagccat gagcatacac atacacatgg tcatgaacat
     1381 tcagatgggc atacacatga acataaataa aaacatttta aatttataag agatttttat
     1441 tttgtgattc catgaaggtt taaataaaaa ttgaatgttt taagtaaaat tattgagaat
     1501 aaataagaaa gtgaactgtt aaagtagata acttaaatag ttcactttta ttaattatct
     1561 aagtattaag tttaaatcca atgattaact aaaaaagaag ctatattttc ataaggtatt
     1621 ttttctgttt ttaataaact ttggacaact aaaccatcaa ctattgacat gagtaaaaaa
     1681 gaaaatgatt cagcatcctg ttcattttcg ctactattat atttttctag tgattcaaca
     1741 atttcagatt taagagtagt tctccattca atatatttta atcttattct ttcctttaaa
     1801 tcattatttg aagtaattgc ctcacaaata agatacatat gtaccctacc acgactttct
     1861 atagtagata ttttctctaa gattaagtta accatttcta tttgagaatt ttttgattta
     1921 acatttttta cacagtttag tatagcacta gttataactt ctaaatgagt atccgcaata
     1981 tcatatataa tatcatcttt agatgcgtag tgataataca aagtaccttt acttatattt
     2041 acatcttttg caatatcagc aaggctagta ttttgaactc catttttggc tattaaattg
     2101 gttgcagatg ataaaatagc ctgtttcata ttatttgttt tcataaaacc tacctttcca
     2161 tgtatattta taacaaatta ataacattat aagcttatgg atatttattg tcaataaagc
     2221 gagaatctat atctattgac tttttttcat tcaatatata aaataataat taaatcggtc
     2281 gtacgaccga ctgaataaat aaaataatat cttatagtga ggttaatatg gataaaataa
     2341 aattgatagt ggacagtgca tgtgacttac ctgatgatat aatagaaaaa tacaacatag
     2401 aagttgttgg tttaaatgta tcttttggag aagaaagtta tatatctgga aaggaaatag
     2461 ataatgaaac tttctataga aaaatgagtg agagtaaagt actaccaaaa acatcatgtc
     2521 cttctcctga taaattttta gaagcatacc attgtcagga agaaagtgta ttagtgatta
     2581 atataagttc aggattatct gggacttata atagtgcact tttagcaaaa gatatgtttg
     2641 aaaaggaagg aaataacaag aaagttgagg ttatagattc acttagtggg tcaattggaa
     2701 taggacaatt agtgttaaaa gcagcaatat taataaaaga aggaaaagaa attgaagaga
     2761 ttgtttcaat aataaataaa tataaagata atcctccatt ttttggaaca cttgaaactt
     2821 tagaaaatgc tattaaaggt ggtagaataa atccaatagc aggaaaaatt ataaatacct
     2881 tgaattttaa ggcgataata caagttgcag atggcgttgt aactcctata gataaggcaa
     2941 gaggcgaagg aaacagtttg aaaaagctga ttactttagt agagtcaaaa attaaagata
     3001 aagaagaaaa ggttttattt ataggacatg ctaattgccc agaaaaggct caaaaggtaa
     3061 gggaagttat ggaaaaggat gtaaaataca aagatgttgt aatctgtgaa gttggttcag
     3121 tgatgggtac atatacttca aagggtgcaa tcttaataac tgcaatataa ataatggatt
     3181 gaaataaatc atagattgag aaattaaagc catataaaaa tgtttaaaac atttttatat
     3241 ggctttttaa ttttatcaaa ttgattttag aattaaccaa ctacacatat ttctttgtca
     3301 tgtttgttta atatctcaca accatcttca gtaacaagta ctaaatcttc tattctaact
     3361 ccgaactcac ctggtaaata tatacctggc tctatagaga atatcattcc tggttgtaca
     3421 gtatctgtat ttatagaaga tacatctcct ttatcatgaa cttcaagtcc tatagagtga
     3481 ccagttctat gagtgaagta ctgtccatat cccttttcag ttatgtagtc acgagcagca
     3541 gcatctatat cacagaatct aacacctggt tttacaatag cttcagctct cttattagct
     3601 tcaagaacta tttcgaatat ttctctacct ttttcaggta tttctttata gaatacagtt
     3661 cttgtcatat cagcacaata gttatctttt atacaaccta tatctaatat tattgcatct
     3721 cctggtttca caagtgcatt tccaggttca ccatgtggat tagctccatt aggtccaaaa
     3781 cctataatag ggtcaaatga aaatccatca gtacctaggt cttcatatat tttagataat
     3841 ttaccaacaa gttgtttttc tgtaagttca ccagaaacag tagctttaag ttgttccata
     3901 gccttgtcat ttaattttga agctattctc attaactctt tttcttcttc atctttacac
     3961 attcttaaag tatctattat ataagatgaa tttacaaact tacttcctcc acctaattct
     4021 attaagttta ataagaatct agctggccag tttttatcaa cacccatagt agcatttttg
     4081 tctatatgtt cagttattat ttctactgga ttttgagtat cattaaacca aaccatttca
     4141 acacctaaat cttctgaaac tgggaataat tcatttataa ataatttatt attaccattt
     4201 aaatttaagt ataatgctaa tagtctttcg cctggatgga ttagtactcc tgtaagataa
     4261 aatatagaag tagggtcact tactaacatt tgagaaatat catctttttt catttgttca
     4321 agtacagcat ttaatctttg agctttcata ataatttcct cctaatcaaa attcaataca
     4381 aatcaatttt agcattatta aacaaaaaag agtatacttt tcttctagaa atataaaaaa
     4441 atttgttata tattttttat attaagttta atctctactt ttgcattaaa tacggaagta
     4501 aaatgacttt ttattgtgaa aattctataa aagtaataaa ttaagtctat aaagttatgt
     4561 attttatctt tttatatact aatgaaaaat aataaataaa attttataat aaaataatat
     4621 tgaaacaatt ttaaaaattt agtatcatta gaattaaagt ggtaaaactt ttaaatttat
     4681 catttttaat aagatttgat ttcaaagtat ttaaggttta ttattaaaac tgtttttgaa
     4741 tatgcaaata ttttgaaaaa tatcagttta ataataatga atctatttta ttaattttga
     4801 aattaaattt aaaataatta atagagaggg gaaattaaat taggatggaa aagaaaaaaa
     4861 taggcctatg gagtttggta tttatgaatg tatcagcttt atatggtata agatggatag
     4921 ctaaatctac agcatcaagc tttggtttag gattaggtgc aatacctatg tggttattat
     4981 tctcatttat atactttgta ccaggagctt taatttgtgc tgaattagct tctacatatc
     5041 aaaaaggtga tggaggtctt tacgattggg ttaaggaagc ctttggagaa aagtatggat
     5101 ttttagtttc ttggttaaac tggacagcta aaatattttg gtattcttca tttttaactt
     5161 tcttagcagt aaatgtttct tatacaatag gaaatccagc ccttgctgat aataagatgt
     5221 ttgtacttat attatcttta ataatattct gggcattatc tcttatagca actaaaggaa
     5281 taagttttac aaaaatattt actaatacag gtgcattagg ttcaacaata ccaagtgcta
     5341 tacttataat attttcattt gtagcagtat ttgtgttgaa aaaacatgat atagcttcta
     5401 catacacaat agcttcaatg actccaaaac ttaatatgga tgcatttgtt gctatatcag
     5461 ctataatgtt tggtttagca ggagcagaga ctgctgctaa cttcataaca gaaatagata
     5521 agcctgaaaa gaacttccct aaggcaatat taatatcagc tggaatagta gcttcattat
     5581 atgtattagg ttcaattgca ataacaatga taataccacc agaccaaatt actgcttcta
     5641 aaggtgtatt agatgcactt tctgctgttt gtgcgtcttt aggaattggt tcttggttgg
     5701 ttcaattaat agcttttggt atagcttttt cagtattagg agcaatagtt ctttacatag
     5761 catcaccaat aaaaatgtta tttggtagtg ttaaaaaagg aatatttcca gatagtttag
     5821 tagaggtaaa tgaacacaag ataccttcta aagcagttat acttcaagca ataatagtta
     5881 ctataatact attggtgact acattaatgc cttctgttga tgctatatac aatgttcttg
     5941 taactatgac agcattaact gcattattcc catatgtatt gttatatgct tcttatatta
     6001 aactaagaaa agagagacca gatgaaatta gaccttatac tatggcaaaa tcaacaagta
     6061 cttgcctagg tttagcaaaa atggttttag ttgtaactgt agtgggtata ttattatctg
     6121 cagcaccagt tatgcctaca tttgctgaaa atgttatata tgagatagag atgattggtg
     6181 gaggtcttct agttatatta tctggtctat ggttatggaa tagatatgaa aagaaaacag
     6241 ctaaataaaa tgtattttaa aaatttcttt tggatattat aatatatagt tgacaattta
     6301 tagtgtaagg aatattataa aataaagaaa ttaataaatt aaatcctgag ataagaaata
     6361 gtaaataaat atatttttgc acagagaatt aaggatgctg agaacttaat aaaaagtatt
     6421 tattgaatgg gtctttgagg agtaaaatga aatgtaattt aagattacag agtatttgat
     6481 atcgtgtatg cacgttatag catatattaa gatatatata ttcaattttg agtgtatata
     6541 aatttaggtg gtaccacgga aaatatatcc gtcctattga tttaggacgg attttttaat
     6601 atagaaaatt taggaggaaa aatgaacgta ataggaatta taggtgcaat ggatgaagaa
     6661 gttagtattt tagttgattt aatggatatt agagagacta taaaaaaagc tagcttagaa
     6721 ttttataagg gaattttgga aggtaagaat gtagtgttag taaagtgtgg cataggtaag
     6781 gttaactctg ctctttgtgc ccagatacta ataagcgaat ttaaggttga tgctattgtt
     6841 aatacaggtg ttgcaggagc tttaaacgaa aaacttgatg ttaatgatat agtaatatca
     6901 actgatgcta tacagtatga tgtagacact acagcatttg gagaccctaa gggagttata
     6961 ccaagaatga agacttctgt atttaaagca gatgaaagac ttatagatgc tgcttacaaa
     7021 tcttctgtgg aagaagtaaa gactcataaa gtattaaaag gaagagtagt tacaggagat
     7081 aaatttataa attctaaaga attaaaagaa gaattagtaa atgattttgg tggatattgt
     7141 ggagaaatgg aaggtggagc tatagctcat gtatgctatt taaataatac tccatttgta
     7201 ataataagag caatgtctga taaggcagat ggaagtgctg atgttactta tgatgtattt
     7261 gtacatgatg ctgccaataa ctctaaagac atagttttaa atatgttaaa atcaatataa
     7321 atatataagg aggtacaaaa aaatgagtga aaaaaaagtt atattaagtg gtgcacagcc
     7381 atcaggcaaa atgactttag gaaattattt aggagctata aaaaactgga ctgaacttca
     7441 agacaattat gattgttatt atagtatagt tgatttgcat gcaataacag tacctcaaga
     7501 ccctaaagtt ttaagagcca atactataga attacttgct caatatatag cttgtggatt
     7561 agacccagaa aaaaatacta tatttataca atcacatgtc aaagagcatg ttgaacttat
     7621 gtgggtattg aatactatga cttatatggg tgaattaagt agaatgactc aattcaaaga
     7681 taaatcacaa aagagtgaag caaatctaaa tgcaggttta ttcacatatc cagttcttat
     7741 ggctgctgac atattgttgt atcaaactga cctagttcca gttggagatg accaaaaaca
     7801 acatttagaa ttagctcgtg atttagcaaa taggtttaat aatagatttt cacctacttt
     7861 tgtagttcca gaaggatact acccaaaagg tggagcaaga gtgatgagtt tgcaagagcc
     7921 aactaaaaaa atgagtaaat ccgattcaaa tgaaaatgca tttatactat tagcagatga
     7981 ttcagattct ataaagagaa agataaaaag aagtgttact gattctttag gtgttgtaaa
     8041 atacaatgat gaacaaccag gtattaaaaa tctaattgat atatattcta atttaagtaa
     8101 aaagagtgta gaagagattg taaacatgta tgaaggaaag ggttatggca tatttaaaga
     8161 agatgtggct gaagtagtat cagaagcttt aagacctata agagaaaaat atgttgacct
     8221 tcttaataat aaagattatt tagaaaaaat atatagtatg ggagcagaaa aagccgaaaa
     8281 acaagctaga aaaactctta gaaaagttta taaaaaagtt ggatttatag agagaagata
     8341 ctaattatta gctatcttta ctttataaat aaattttaaa gttattaaaa aggtatatga
     8401 tatgaaagag agagtctcaa agtgaacttt tgttcatgag gcactctttt ttgtatgaaa
     8461 taaaatatat tttaatcatt tcaacaatga aaaagaggat tatctctgtt ttgagacagc
     8521 atcttttgat aataaaaaaa gatgagcttt tgctcatctc aaaggggtta gattattctt
     8581 actttgggga aagtagaata aaggtaaaag ttaactgatg tcaactttgt cttttttgca
     8641 tatcactact gagcataaaa aaatcagaaa accatagcct tctgaaacaa cttctaacat
     8701 acattgtatt ttatacccaa tatgtaattt atgagttaat tttaactctt ataatgaaaa
     8761 ttgtcaataa tatatcaaaa aaatattttt taaatttata taattaaatg aatttatgtt
     8821 tatatatagg tgatatgata cagataatta tctttttaat acaatatagt tctaaaaaag
     8881 aatatattag aagaagataa tatttaaata aaaaatacct tataaagtta attttttaat
     8941 actttataag gtaaatacat attgtattgt ggggataaat ttaaatcatt tgatactaat
     9001 caacactaag aaccaataac tctctatcat cttgagtaat tgtatatatt cttaatttct
     9061 tgtatgtcat aatattttct ccacctgtaa aataactcct aagattaaca caatcagttt
     9121 taggttgatt tgtatctcct aaataattac gcaattcttt atctttagtt tcaatatctt
     9181 taaatccaaa atattgtaaa gtagcttcaa ctctatattt ttggacataa tcgagagctt
     9241 ctttattagt ttatgttggt gtaaaataca taccatttat ataagccttt attgtacttg
     9301 gattaaattt aaaatctgca gaatatattt tatgtgtaac tattatttct tgaacagttg
     9361 gaattttaac ttctggttca tcaagtattt caggagtact atttagctct gtaatagata
     9421 agttatctaa gtatattgta tcactatcaa ttaatgctat atttatatta gaagaatctt
     9481 tttcagtagt ttcaaattca taactaaatt ttgtatatcc ttgttctagt accaaataat
     9541 ccgttttttc ttcttttact tttatattta caattattga attatatgtt aaaggatttt
     9601 ttgaatttta ctaaaaacat aacgtttata aggttttagt tcagacatag gtattttaat
     9661 cttcgtctca ccatttaatc tatttgctga gccttttaaa ccatcttgat ttgtagtatt
     9721 aacattactc catatactag ggtaattatt gtaatcatca aaattagtaa agtaagttgg
     9781 tgtttttata agtatattca ttcctctttc aagtttaaca ttacatattt gtttatcaga
     9841 caaagttttt aaactgtctt taatcttatt gcctatatta tcatcaaata tgagttcaac
     9901 acaactttca tctattggta tattattaaa atataacaaa ccatcttttt tagtagtgcc
     9961 aaaagttttt tcaattgctt ctccaattgt aagttcaggt gttttatctt ctgggacctg
    10021 taaactttta gcagtaattc ttcttttgta agattcattt tctgtatcta atataataga
    10081 tacagaaata ctgtcaattt gacctatatc gtcttaccaa ctatttcatt ctgttactat
    10141 ttgtccagaa ccattttttg taccaaaatt tccacttact tgtgttgttt ctaatttaat
    10201 ttgctttcca tcatctaatt tttttaatta attataattt ataggaatca gtctagaact
    10261 aaattgatac attgtgttaa gagccatagg tgaaaatcct tttttgagat aagtatttcc
    10321 aggagataga ttattgccaa tttgattttc ttgtgctttg atagttgata atgcatctcc
    10381 atctaacact aaatttgttg ttggtgtcac tttgtacata ggtgcagtac ctgtgttgta
    10441 gtatctaata tttgcattta tatatgcaga ttctcctttg tttatactta atccagtatt
    10501 tcatgattat ccattgctat tttgaacagc agttgaatta tctgttgtat ggtaataatt
    10561 tgtcttgtat agtttcttta aactcattaa atttttctaa tgaaatttcg ttttgatttt
    10621 ttgttcttat tactttatta aatgcaaatt tttctggaga ttcaaaataa tatacataca
    10681 taggtttatc tattttattt tttgatatag catttcgaag ttctctatat tctttttctc
    10741 aagaatttgc ttctatttga taatcataaa aataatttct tgtctgagaa tatttgctta
    10801 tctctataga atctttttta tatgatccta atacttcttt ttcagatttt tcaagttttt
    10861 gatcttttct ttctcattct ttagcctttt ctttatcttt aaaaaaatcc ttctgatctt
    10921 tctataggag ccttgtaagt agtattacaa acttttccac tatagctttg taaatcttgt
    10981 gcataaatgt tattaggtaa caaactacct aaagttagat ataatatcaa caatatagat
    11041 atacaattac taatactttt atgtttccta aattttttca tttattctcc ctacaaatat
    11101 tagtttagtt aacttttgaa ttttaat
//
LOCUS       DAELJO010000131        11107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELJO010000131 DAELJO010000000
VERSION     DAELJO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.131"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            404..1726
                     /gene="dnaA"
                     /locus_tag="KPB88_002702"
     CDS             404..1726
                     /gene="dnaA"
                     /locus_tag="KPB88_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG0054969.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1968..3074
                     /locus_tag="KPB88_002703"
     CDS             1968..3074
                     /locus_tag="KPB88_002703"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG0054970.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3211..3417
                     /gene="yaaA"
                     /locus_tag="KPB88_002704"
     CDS             3211..3417
                     /gene="yaaA"
                     /locus_tag="KPB88_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG0054971.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3435..4550
                     /gene="recF"
                     /locus_tag="KPB88_002705"
     CDS             3435..4550
                     /gene="recF"
                     /locus_tag="KPB88_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG0054972.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4551..6452
                     /gene="gyrB"
                     /locus_tag="KPB88_002706"
     CDS             4551..6452
                     /gene="gyrB"
                     /locus_tag="KPB88_002706"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG0054973.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMIAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6472..8898
                     /gene="gyrA"
                     /locus_tag="KPB88_002707"
     CDS             6472..8898
                     /gene="gyrA"
                     /locus_tag="KPB88_002707"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG0054974.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8967..9263
                     /locus_tag="KPB88_002708"
     CDS             8967..9263
                     /locus_tag="KPB88_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0054975.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9263..9523
                     /locus_tag="KPB88_002709"
     CDS             9263..9523
                     /locus_tag="KPB88_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG0054976.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9535..9852
                     /locus_tag="KPB88_002710"
     CDS             9535..9852
                     /locus_tag="KPB88_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG0054977.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYILNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9877..10263
                     /locus_tag="KPB88_002711"
     CDS             9877..10263
                     /locus_tag="KPB88_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0054978.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10263..11036
                     /locus_tag="KPB88_002712"
     CDS             10263..11036
                     /locus_tag="KPB88_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG0054979.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4511 a         1150 c         2017 g         3429 t
ORIGIN      
        1 gtttatattt ttatcaacaa ttagttaata ttgtggataa attagttaca aatcattgaa
       61 gtttgacata tattttgata ttataaatat acttgttgat aatgtgcata aatgttatca
      121 acttattaga aattattcac agcttgtgga tacttttgtg tataactttt aagattactg
      181 acaatactct agttataatc accatttatt gtatgtttat gttatttata actttatcta
      241 catattgttt atatcttttc atattttttt aataatttat actattatta atttatcaac
      301 accattatcc ataaatttag ttattctgta aaatttatat atatttttgc ttttctgtta
      361 ataactttgt ggaaaatatt aattattctg ttcggaggtt tatattatgg atatagtttc
      421 tttatgggac aaaaccctac aattaataaa aggtgactta acttcagtaa gttttaatac
      481 cttttttaaa aacatcgtac cattaaaaat acatcttaat gatttaatct tactggctcc
      541 tagtgatttt aataaagata tcttagagaa tagatattta catttaattg aagatgctat
      601 tagtcaactg tcattaaaaa aatataatat taaatttgta ttgagcgaaa aagaggttgc
      661 tgatttaaat tctgatagca cagatttaaa ttatagagtt ctttacccca atctaaatcc
      721 aaaatacaca tttgatacat tcgttattgg taatagcaat agatttgcac atgctgcatg
      781 cgttgcagtt gcagaatctc cagctaaagc ttataaccca ctttttttgt atggaggagt
      841 tggattaggt aagactcacc taatgcatgc tataggtcat catattgtta gtcaaaaaaa
      901 agactcaaaa gtagtttacg tttcttcaga aaaatttact aatgaactta taaactctat
      961 aaaagacgat aaaaacgaag aatttaggaa taaatataga aacgtagatg tattactaat
     1021 cgacgatatt caatttatag caggaaaaga aagaactcaa gaagagtttt tccatacatt
     1081 caatactctt catgaagcaa acaaacaaat aattatatct agtgatagac cgccaaaaga
     1141 catacctaca ctagaagata ggctaagatc gagatttgaa atgggactta taactgatat
     1201 tcaagcacct gactttgaga caagaattgc tatacttaga aaaaaggctc aactagaacg
     1261 tatagatgtg ccaaatgagg ttatgtcata tatagcaaaa aatattaagt caaacataag
     1321 ggaactagaa ggtgcattaa ctagagtggt tgcttattct tctcttagca atagggtaat
     1381 atcttttgat cttgctacag aagctttaaa agatattata acaacttcta aaaatgaaga
     1441 aataaatgtt ctcagaataa aagaaaaagt atcttctgta ttcaatttaa agatggaaga
     1501 ttttaattct aaaaaaagaa cacgttcaat agcttatcca agacagatag ctatgtattt
     1561 aacgcgcgag cttactgatt tatcccttcc taaaataggt gaagagtttg ggggtagaga
     1621 ccatacaaca gtaattcatg ctcatgacaa agtatctaag gatatagaag aaagtgaaga
     1681 aattaagact aaaatagaca aaattatatc agatttgaag ggataattta tacacaattg
     1741 tatgtttata tctcttgaat aacctgttaa tacttttttt gaacaaaaat tattaacaaa
     1801 ttatcaagtc taaaataaca aagatattaa catcttatac acatgttaat atctttgtat
     1861 ttataatgtt atacatactt attaacatta tcaacaacac ctactactat tactactatg
     1921 attttaactt atttttatat ataaaaagcc agaggaggtt atccacattg aaaataattt
     1981 gtaatcaaaa aatcctagca aatagaattg gaattgctca aaaagctatt aatggaaaaa
     2041 caacaataga attattaaaa ggtatactta tatctacaga agaaggtcaa ttaaaactta
     2101 ctggatatga tgctgaaata ggaatagaaa cttatgtaca agcagaaatc atagaaaaag
     2161 gtgatgttgt tgtagatgca aggctatttg gagatataat tagaaaatta ccagattcct
     2221 ttgttgagat agaaactgat tcagaaaata atatttacat aaattgtgta aattcaagat
     2281 ttaaaattaa aggttatgca gcaaaagaat ttcctaaatt accagaatta aatgaagaag
     2341 atttatatag catacctcaa gaaatcctaa aaaatatgat taaacaaact gtatttgcta
     2401 tctcacagga ccaaacaaaa cctgttttaa tgggagaact tttagaaatt gtagatagaa
     2461 acttaaattt agtagcaatt gatggatata gattagctgt aaaaagttgt tcagttgata
     2521 gtttaactga aaatataaaa gtcataattc ctggaaagac acttatagat gtaaatagtt
     2581 tgctttctgg agaggataat gttaaagttg gatttaacga aaaaaatgct atttttataa
     2641 ttaatgatac gaaaattatt acaagattgc ttgaaggaga ttttattgat tataaaaaat
     2701 tattgccaag agaacataac agcagagtta aattaaatac taaggaactt ttaaatagta
     2761 tagagagagc gtctttattg tctcaatcag aaaaaaataa tcttataaag ttatctataa
     2821 gagataaagt aatggcaatt acttctaata cagaaaaagg aaatgtgtat gaagaagttg
     2881 aaatagattt agatggagat tacctagata tagctttcaa ttctagatat tttatcgaag
     2941 gtttgaaaaa tatagataat gaggaaatat ttatagaatt tactacaaat gtaaatcctt
     3001 gtataattaa accaacagat gatgttaatt atatttactt actgcttcca gtaagaatat
     3061 catcaaatat atagcagaca tgaaggagtt gtcttttgta gaaaattttt ctaatcttag
     3121 acaactcctt ttataagcat atttatggta taatatactt ttagtgtatc aataggaatt
     3181 tgtttaaaat agtgacaagg aggaactatt atgactgaaa taactataga atcagaatat
     3241 ataaagttgg atcaatttct taaattagca gagattgcat caacaggagg tcatgcaaaa
     3301 tttttaattc aagaaggttt agtgacagta aatgatgaaa tagaattaag aagaggaaaa
     3361 aagataaaat caggagatat agttgaaata gaaggaacta aaataaaagt attgtaaaaa
     3421 aaggagaact ttttgtgaaa cttaaaagtt tgcaacttgt taattttaga aattataaaa
     3481 agttgcatct ggaatttaat gggaaagtaa atctacttgt tggtaaaaat ggtcaaggaa
     3541 agacaaatat agtagaatct atttatatgc tttcttttgg aaaatcattt aggactaaca
     3601 aagataaaga aatggttaga tttaatagtg aaaacttata tattggagga agtttttcaa
     3661 aatataataa atatagtcta atagagctaa ttattggaaa agataaaaaa gggataagaa
     3721 taaataaagt tcctttacaa aagatacaag aactacttgg caacttaaat gttgttatat
     3781 tttctcctga ggatttaagg ttggttaaag agggtcctaa agaaagaagg gcatttatag
     3841 acaaagagat tagtcagatt atacctaaat attataaata tcttaccaat tataataaaa
     3901 cactttctca aaggagtagg gtgcttaaaa atatacatgt tgatgaagct ttattagatg
     3961 tatatgatga tacgttagca aagtatggaa gttatattta tattttgcgt agagacttta
     4021 taaaaaagat agctaatata tctgaaaata tgcatatgaa tttgactaat ggagttgaaa
     4081 gattatcaat tcgatataaa aatcaaataa atataactga tgaagatact atagatacag
     4141 tatataataa atttttagcc aagttgtcat cgaatagacc taatgatata gaatctaaga
     4201 ctaccagata tggtatacac aaagatgact taaatatatt cataaatgat ttagatgcaa
     4261 gactttttgg ttcacaaggt caacaaagaa ctgcttcaat atcattaaag ttatcagaga
     4321 tagaattaat aaaaaatgaa gttgaagaat atccagtatt aattttagat gatgttttta
     4381 gtgagttaga tgaagcaaga caaaaacttt tagtaaataa tttaagcaat gttcaaatgt
     4441 ttataacaag tgctgaagtt tcacataaaa aaatatttga tgaaaaaaat gttacaatat
     4501 ttaatattga aaatggcgat gttattagca tagagaatgg aggaaattaa atgaaacaag
     4561 aatacggtgc aagtcagata caagtattag agggattaga agccgttaga aaaagaccag
     4621 gtatgtatat tggtagtaca agccctagag gtttacatca cttagtttat gaagttgtag
     4681 ataatagtat agatgaggct ttacaaggat attgttcaga catatatgta tctattaatg
     4741 aagatggcag tgttttagta aaagataacg gaagaggtat acctgttgaa atacatccta
     4801 aaactggtaa atcaacttta gaaacagtat taactaatct tcatgcagga ggaaagtttg
     4861 gaggcggagg atataaagta tctggaggtc ttcatggagt tggtgtttct gtagttaatg
     4921 cattgtcaaa atggatgata gcagaggttt atttaaacgg aaagatatat aagcagacat
     4981 atgagaaagg attgccaaca tctaagcttg aagtagttgg agaatctcaa gataaaggaa
     5041 caatgattca atttatgcca gatgagacaa tatttgatga aatagagttt aaatatgaaa
     5101 cattggagta taggcttaga gaactttctt tcttgaataa aggaataaaa atagtatttg
     5161 aagataaaag agaaggacaa gaaaaaagaa aagaatttca ctatacaggt ggtttggtag
     5221 aatatattaa gtatttaaat aaatctagaa ctggtataca tgatgacata gtttatatag
     5281 ataaaaaggt tgatgattgt tttgttgaac tagctatgca atatacagat ggttatacag
     5341 agaacatata ttcttttgca aacaatataa atacacatga aggtggttct catttaagtg
     5401 gatttaaagc agctcttact aaaactgtaa atgattatgc taagagaaat aaatttttaa
     5461 aggaaaatga tgttaaccta cttggtgaag acataagaga aggtcttaca gcagtagtat
     5521 cagttaaatt accagaacct caatttgaag gtcaaactaa aacaaagcta ggtaatagct
     5581 ttatgagagg tatagttgat agtgtaacag ttgatgaact ggggtctttc ctagaagaaa
     5641 acccatcaac agcaagaatc atagttgata aagctttaag agcacaaaga gctagagaag
     5701 ctgcaaaaaa agcaagagaa ttaacaagga gaaaaagtgt attagaaagt acatctttac
     5761 ctggaaaact tgcagattgt gcagaaaaag atccatctaa aagtgaaata ttcttagtcg
     5821 aaggggattc agcgggaggt tcagctaaac aaggtagaga tagaaatagt caagctatac
     5881 ttccattaag agggaaaata cttaatgttg agaaatcaag actagatagg atactatctt
     5941 cagatgaaat aaaaaatatg ataacagctt atggttgtgg tattggagaa gattttgata
     6001 tagataaggc taggtatcat aaaattataa ttatgaccga tgctgatgta gacggagccc
     6061 atataagaac tttattatta acattcttct ttagatatat gagacctctt atagatgagg
     6121 ggtatgttta tgcagcacag ccacctttat ataaagtgac aaagcaaaag aaggaacatt
     6181 atgtttactc agataaagag ttaaatatct tactagatga aattggaaga aatggtgttg
     6241 aacttcaaag gtacaaaggt cttggagaga tgaatgcaga gcaattatgg gaaacaacta
     6301 tgaatcctga gacaagaact ttattacaag ttactgtaga agatgctgca attgcagatg
     6361 aagtattttc aatgcttatg ggtgataagg ttgctccaag aaaagaattt atagaagaaa
     6421 atgcaagatt tgttagaaac ttagatatat agagagaggg gattagtata catggaagaa
     6481 aataacaaaa tactccctat tgaaatagcg gaagaaatga aaaaatcgta tattgattat
     6541 tcaatgagtg ttatagctgg acgtgctctt cctgatgtta gagatggttt aaagccagtt
     6601 catagaagaa tattatattc aatgagtgag ttaaatttaa ctccagataa accatacagg
     6661 aagtcagctc gtattgttgg ggacgtttta ggtaagtacc atcctcatgg agatactgct
     6721 gtttattatg ctatggtaag aatggcacaa gatttttcaa ctagagcact tttagtagat
     6781 ggtcatggta actttggttc tgttgatggg gattcaccag ctgctatgcg ttatacagaa
     6841 gctaaaatga gtaaattatc attagaacta ctgagagata ttgaaaagga aactgtagac
     6901 tttaaaccaa actttgatga gtcgttaaaa gagccttcag tattgccagc tagatatcct
     6961 aatttattag taaatggctc aaatggtata gctgttggta tggcaacttc aatacctcca
     7021 cataatttag cagaagtaat tgatgcaact gtatatttga tagataatcc agagtgtagt
     7081 gtagatgatt taataaaatt tgttcaagga ccagatttcc ctacagctgc aattataatg
     7141 ggaaaagaaa gtatagcaga agcatacaga actggaagag gaaaagttaa agttaggtct
     7201 agagctttta tagaagagct accaaaagga aaacagcaaa taatagttac agaaatacct
     7261 tatcaagtaa ataaggctaa actggttgaa agaatagcag agttagttaa agaaaagaga
     7321 atagaaggta tatcagacct aagagacgaa agtaatagaa atggtatgag aattgttata
     7381 gaattaaaga gggatgctaa tgctaatata gtattaaata atttgtataa acattctcaa
     7441 atggaagata cttttagtat aataatgctt gcacttgtag atggtcagcc aagagtttta
     7501 aatcttaaac aaatattata tcattatatt aaacatcaag aagatgttgt tacaagaaga
     7561 actaaatttg aactaaataa agctgaagca agagcacata ttttagaagg attaaagatt
     7621 gctttagata atatagatgc tgttataagc ttgataagag cttcaaagac tgggcaagaa
     7681 gctaagctag gtttaataga aaaattcaaa ttaactgaaa tccaagcaca agctatatta
     7741 gatatgagac ttcaaagact tacaggttta gaaagagata agatagaagc tgaatatgaa
     7801 gatttaatca agaagataaa tagattaaaa gagattttag ctgatgaaag attactttta
     7861 aatgtaataa aggatgaaat tacaataata aaagaaaatt actctgatga gagaagaaca
     7921 gaaataagac atgctgaagg cgaaatagat atgagagatc ttataagtga tgaagaaata
     7981 gcaataactc ttactcactt tggatatata aaaaggcttc catctgatac ttataagagt
     8041 caaaaaaggg gtggaagagg tatttcagca cttacaacta gagaagaaga ttttgtaagg
     8101 cacttggtaa ctacaactac tcatagtaga ctattattct ttacaaataa aggtagagta
     8161 tttaaattaa atgcatatga aataccagaa ggtaagagac aggctaaagg tactgctata
     8221 gtgaatttac ttcaattgtc agcagatgag aaaattgcta ctctaatacc tattgatggt
     8281 aatgatgaaa atgaatattt attacttgca acgaaaaaag gtattgttaa aaagactaag
     8341 agagaagagt tcaaaaatat aaataaatct ggtcttattg caataggttt aagagatgat
     8401 gatgagctta ttggagtaga acttacagat ggaaaacaag aagtactttt agtaactaaa
     8461 gaaggtatgt ctataagatt tgatgaaaat gatataagat atatgggtag aacagcaatg
     8521 ggtgtaaaag gtataacttt aagtaaagaa gattttgttg tgtctatgaa cctttgcagt
     8581 aaaggtacag atgtgttagt tgtaagtaag aatggttttg gaaagagaac aaatatagaa
     8641 gagtatagaa gtcaaataag agctggtaaa ggaattaaaa cttataatat atctgaaaaa
     8701 actggtacaa ttgtaggtgc agatatggtc aacgaagatg atgagataat gattataaat
     8761 tctgatggag ttcttattag aataagagtc aatgaaatat cactgtttgg aagagttaca
     8821 agtggtgtta aattaatgaa gacgaatgat gaagtcaatg tagtttcgat tgccaaaata
     8881 aatattgaag aagaatagga taaatatcct attctttttg tataatgtag ttagataaaa
     8941 atttaaaatt gaaaggagta attgttatgt gcaataaaag aagttgtaaa aaaggtggat
     9001 ttttattatt aggagctatt ttagggttta tatttggtat gttttttgca cctaaaaaag
     9061 gttctgaact aagaaaagaa acaaaagaga agtttaatga tgtcaaagaa aatccaaaag
     9121 aagttctaca tgaaacattt aatgatgtta aagagagaat aataaattta gtggatgatg
     9181 ataataatga agaagatata aagatttcag aagaagatat agtaataagt aaaagctttg
     9241 atgatgaggg agatgttaac taatgaatgc atgggggtgg caaattggag cagtgttagt
     9301 aggaagctct gctcttatag tagcaattta tttagctaaa actctaaaca gcataaataa
     9361 ggttgttgaa aaggcatata agattgtaga ttataatgaa agacatatac aagatattgt
     9421 tgaaaatgca tcatcaatat caaagggaat agatgatata gtttatgtaa ttaataaaat
     9481 attgagtata ggaaatatat ttaaaattat taaaaggaaa taggaggttt ttacatgtct
     9541 atgaatattg attcaaattt agattcacaa aataaatttt ggaatgtttg tcttgatgga
     9601 gaacttgatg tttctacagc agataaacta aaggaacatt tacatgcttt aatagaaaaa
     9661 aatatgttag atgtaaagat taatcttaaa gatttagatt atatagactc aacaggctta
     9721 ggagctatga taggtgtatt aaaaaaatta aaaataaatg aaaaagagat atacatatta
     9781 aatcctaaga gtaacgtaag aaagattttt actataacag gtctagataa aatatttaaa
     9841 gtggagggat aaactttggc ttgtgagact ataaagatgg aaattactac aaatccagat
     9901 tatgttagta taataagatt aacagcatct ggaattgcca ataagatggg ttttccaata
     9961 gatgatatag aagatataaa ggtagcagta tctgaagcat gcacaaatgc aataaaacac
    10021 agtaaagatg atgtgtttaa tatagtattt aatattttag ataatgcaat taatatagaa
    10081 attcaagata atggaaaagg atatgatgtt agctcaataa gtacaccaga tttagagaat
    10141 ccaaaagaga gtggtcttgg attgtttata attaaaactt taatggatga tgtagatatt
    10201 gaatcagaac ataatcaagg gacaaaaata aaaatgacta aatatttagg agttgatatt
    10261 taatgaaaaa tgtagctaat gctacacatt acctaaatat ggatacaaaa gagctattta
    10321 atttatataa taaaaataaa aatgtagaca ttagaaatat tcttattgaa agacatctgt
    10381 atttagcaag attgttagct aagaagtata taaacaaagg tgttgatttt gaagatatat
    10441 atcaagttgc ttcattggca ttaatttatg ctattgatag atatgatgtt gagaaaggat
    10501 ttgagttctc cagctttgca acaccaacta tagtaggaga aataaaaaag tattttagag
    10561 ataaagtatg gacacttaga gtcccaagaa gaatacagga attaagcaaa aagataagtg
    10621 atgcgaagat taaattagaa caagaaaata agaagcatcc aaaggtaaaa gatatagctg
    10681 attatatagg cgttagtgaa gaagatgttt tagaggctat ggaggcttct tatggatatc
    10741 aaccaatgtc tttggattca tcaagtaatg atgattcaga ggataaagat ataactctta
    10801 ttgataagat tggaaaagaa gaaggtaact ttggaagtat agagtatgaa gattttatta
    10861 ataagtttat tgaaacttta aatgaattag aagttaaaat ttttaaagat agatttttct
    10921 ttgataaaac tcaatctagt atagctaaag aactagggat atcacagatg actgtttcta
    10981 gactagagaa aaaggtagta gagaagttga aaaaagaata tgaaaaaaat ttataaaaaa
    11041 atataaaaaa agtattgacc tactgtttta aagatggtat agtattactt gtccttgaaa
    11101 aaaggaa
//
LOCUS       DAELJO010000132        10905 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELJO010000132 DAELJO010000000
VERSION     DAELJO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.132"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(108..3215)
                     /locus_tag="KPB88_002713"
     CDS             complement(108..3215)
                     /locus_tag="KPB88_002713"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG0054980.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3308..3540)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3715..4242)
                     /locus_tag="KPB88_002714"
     CDS             complement(3715..4242)
                     /locus_tag="KPB88_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG0054981.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4277..5062)
                     /locus_tag="KPB88_002715"
     CDS             complement(4277..5062)
                     /locus_tag="KPB88_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG0054982.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5072..5329)
                     /locus_tag="KPB88_002716"
     CDS             complement(5072..5329)
                     /locus_tag="KPB88_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG0054983.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5348..5806)
                     /locus_tag="KPB88_002717"
     CDS             complement(5348..5806)
                     /locus_tag="KPB88_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG0054984.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(5846..6550)
                     /locus_tag="KPB88_002718"
     CDS             complement(5846..6550)
                     /locus_tag="KPB88_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG0054985.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6560..7195)
                     /locus_tag="KPB88_002719"
     CDS             complement(6560..7195)
                     /locus_tag="KPB88_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0054986.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7576..8616
                     /locus_tag="KPB88_002720"
     CDS             7576..8616
                     /locus_tag="KPB88_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0054987.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9092..10447)
                     /locus_tag="KPB88_002721"
     CDS             complement(9092..10447)
                     /locus_tag="KPB88_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG0054988.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10473..10742)
                     /locus_tag="KPB88_002722"
     CDS             complement(10473..10742)
                     /locus_tag="KPB88_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0054989.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         3290 a         1984 c         1213 g         4418 t
ORIGIN      
        1 ggaatattaa aacattttat tgaatagata attatattac ataaaaaatc cctatagaaa
       61 tctataaact aatttctata gggattttgg ttaactaact attcttttta tttttttata
      121 acttttatac ctgtcatatg gtcatttaag ttttgttcct ctaaagattt atcatctact
      181 ctgtttattt ctaaagctaa agtttcagac ttgatatatt cattaaaatg gtcaacagcc
      241 ttagcaattt catcatcacc acagtattct attactatat tatctaatac ttcaaagtta
      301 ttagattttc ttaattgctg tactttagat ataaattctc tagcataacc ttcattaatc
      361 aattcttcag ttagcttagt atctagtata acaaaaagat tgttttcaac agatacatta
      421 aatccttcct tagcagttat acctattaat actaaatcct tattaaattc aaaagcttca
      481 ccatctaaat caactgttaa agtctcacct ttttcaagtt tattagcagt ttcatgagca
      541 tttaatttac ttaatgctcc aacgaataaa ttcatcttag aacctaataa tggccctgct
      601 tccttaaagt ttggttttaa actaaagttc atatactcat ctaaatcctt agcaaatatt
      661 acctctttaa cattaagctc ttcttttatt aaatcaacta catcacttat tgtatcttca
      721 aacttaccat ctactaacac tttttgtaat ggttgacgaa cttttattct ttctacttct
      781 cttgaagctc tacctaaagt aactaagttt ttagctaaat ccattttttc ttctaattta
      841 gtatctacta actcttcatt acatttagga tattcagcta aatgaacaga tacttctcct
      901 gttaagtttc tatacatttc ttctgacata tatggtgcaa atggagctat agctctacaa
      961 agaccatgta acaattcata agttgtattg tacactgctt ttttatcttc tgttaattca
     1021 gttgcccaga atcttcttct tgaacgtctt atataccagt tagataaatc atcatttata
     1081 aaatcttgta tcattcttaa agttttattt aactcaaata tagctaaatt ttcttctaca
     1141 tctttcatta aattattgaa cttagataat atccatctat caagttctgg tctatcttta
     1201 tattctacaa aaaattcagt tggatttata tcatctgtgt ttgcatataa agtaaagaag
     1261 ttatatacat tcttcatagt acctataaat ttactttgga tttcttttaa tccatccata
     1321 tcaaatttag ttggagtcca tggtggagaa acatatagta aataccatct taatgcatct
     1381 gctccatatt ggtcaaataa ttccattgga tttactgtat ttcctcttga tttactcatt
     1441 ttcttaccat ctttatcaag tacaaggtct ggaactaaaa cactcttata tggtgctttt
     1501 cctaatacaa atgttgatac tgcaagtaat gagtagaacc aacctctagt ctggtctata
     1561 ccttctgata tatagtcagc tggaaacaat tggtcaaagt tttccttatt ttcaaatgga
     1621 tagtggtgtt gagcaaatgg catagcccca ctatcaaacc aacagtctat aacctctgtt
     1681 actcttgtca ttggttttcc acatttttca cattttaagt gtatatcatc tacatatggt
     1741 ctatgaagtt caactgtttc tggatttaca tcttctatag ctttttcagc taattcagct
     1801 cttgaaccaa ctgaatctgt atgaccacat tcacatctcc atataggaag tggagttccc
     1861 caatatctag ttcttgatat tgcccagtca tttagatttt ctaaccagtt tccaaatctc
     1921 ttctctccta caaaacttgg gaaccatttt acaccattat tattttctat taatttatct
     1981 ttaagtttag tcatctctat ataccagctt ggttttgcat agtatactaa tggagtttga
     2041 catctccagc agtgtggata attatgaact actttttctt tgctatataa tttattttca
     2101 gaagctagcc attttattat ttcaacatct agcccatctt ccattacaaa tctaccttcc
     2161 caaggagtag atgtaaattt accagtttca tctactggtt gaataactgg taaatcatat
     2221 cttttacctg tgttgtagtc atcttcacca aatgctggtg ctgtatgaac tataccagtt
     2281 ccatcttcag tagttacata atctcctact gtaataaaaa atgctttttt gtcaacttct
     2341 acaaaaggca ttaattgttc atattctaaa ccttctaggt ctttaccttt catttcttct
     2401 aaaacttcat aatcttcacc tagtacttta tcagctaaag gtttagatac ataatatacc
     2461 tcatcacctt ttttaacttt taaatagtct acatcagcat ttactgttaa tgaaacattt
     2521 gatggtaaag tccatggagt tgttgtccat gctaagaagt attcatcagc atcttttttc
     2581 ttaaatttag ctataactgt attagttttt acttctttat atccttgtgc aacctcatga
     2641 ctagcaagac cagttccaca tcttgggcaa taaggaagta ttttatgccc ttcatatata
     2701 tatccttctt tgttgaattt gtttagtatc caccatacag actctatata gttattatct
     2761 aaagttatat atggattatc taaatcaact tcataagcca ttctctcagt cattattctc
     2821 cattgttttt caaatgagaa aactgactct ctacattttt ggttaaattc agctattcca
     2881 tattcttcta tttgttgctt actagtaagt cctaactctt tctctacttg tatttcaact
     2941 ggtaatccat gtgtatccca accagctttt ctctttactt gataaccaga cattgtttta
     3001 tatctacaaa ctgaatcttt aagcgttcta gataaaacat ggtgtactcc aggatttcca
     3061 ttagctgttg gaggtccttc atagaatacg aaacttgggt catctttccc tttttctaaa
     3121 gttttttcaa gtatatttat gtctttccaa taatcaaaaa cttgtgcttc tgcttgtttt
     3181 acagaactgt ctaccaatgg cttaaatttc gccatgtaaa tcaccctttc ctactaaata
     3241 ttataattcc aaaatttttt attctttcac ataaatttaa atactaaaaa actcgccccc
     3301 tttaaaccaa agggacgagt tataaatcgc gttaccaccc taattctata tgtaaactaa
     3361 atactagcta catataacac tctaatagta taacggtact aaccggcaca gcttagtaga
     3421 atttaatcgt tcagcctgca gctcaagagt gatttttatc tttataaatt cattggttct
     3481 cagcataccc aattctctgt aaaattatct aaagataaat gtctctatca acgcttttaa
     3541 aaatttatct gtgtttatat gaaattctat aacaaataat ttgaatagtc aatatatttt
     3601 tgaaaaatat aataatttct atttttctta catttttatc ctatttttaa tctttgttat
     3661 agttttctca aactcacaca attattctac ataaaaaata aacaaattac aagcttattc
     3721 taaagttgta gcagcttcat cattactata attatacact gttgttcctt caaatgcatt
     3781 tacacatttt tcatctacat cataaaaaat ctcatcaaca cttcttattt catcttctaa
     3841 cagtgattta aatttatttc taaatatctt aaattcttta actaaagcat catactcttt
     3901 tcttatctct ataacacggt tattagcttg ttctataatt tgtctagctt ttaaatcagc
     3961 ttcttcaaca attatcttag cctttttatt agccgcgcta caagtgtctt ctgctgcact
     4021 ttgagcagtt attaaagtag cttttagtgt ttcttcaata ttttcatact tgtttacttg
     4081 gtcttgatat aaccctaatt tttctttaag agctgtattt tccctacata gagattcata
     4141 atcttccttg atgatatcta aaaattcatc aacctcttca tctctatacc ctcttaaacc
     4201 ttttttaaac tctttatttt ctatctcaat tggagttagc attacatttc ctcccagcta
     4261 taatttaaat tagtccttat acaattaatc ttgcttgaac ttttattttt ccttttttag
     4321 taatatctcc tatttgtgtt accttagctc ttcctttgcc tctaatagaa atcaaatcat
     4381 cgttttttac tattttagaa gttgaagtta tcttttcata attgacatgg actttttctc
     4441 catttatgta ttttgcacta tcttgtctag atatattgta aataccactt atcacacaat
     4501 ctaacctatc tgaagatact gtaaatgata cttctttata ttttgaagaa ctattaacaa
     4561 tattttctct ggaaatttcc tttacaatta cattatttct tgaaaccttt tctaagttta
     4621 caattataaa atcgcaaata tcactactta caactacttg acaaaagcta tcatgtataa
     4681 ttatatctcc aattttttct ctttttatac ctagacccag tattgaccct aagtaatctt
     4741 tatgagatat atttttaaat ttgaaattcc cttctatttg taaaaatcta agagtatctt
     4801 ctatatcttc atattccatg taaaatggat aaataaatac agtgcttctt tcagcttgtt
     4861 cataacctcc atccacactg tattttattt catttgtaga atttaatatt gctacagcat
     4921 ttttcacttc atatggatta agaaactccg tagacttaac atcatagttt tttatacaac
     4981 tattagcctt gtctatcact ttaaacattt tatttttcaa gtctatatcc tttatgtgat
     5041 ttgttagttt taatttatcc atcctatcac cttagaatat aaccaatact aattgtctta
     5101 ggaacatcaa taatactatt gcaattaaag gagaaaaatc tataggtcca tttgtatatt
     5161 tatacattat agaccttatt ggcccttcta ttggctctgt aagattttcc aaaatatcaa
     5221 aaattttact ttgtcttcca tttggaaacc aagtcatcaa gctttttata actatcatcc
     5281 atgctattat atctaaaaga taataaagtg caattcttat tgttcccata tattaaccgc
     5341 ctttttacta tttattttgc caagggaaga atgctttact ttctaattct tttttcatat
     5401 ttgcatctat atccacatta ttaggagcta gtataaatat tgatttagaa accttttgta
     5461 tacttccatc taagacataa actgccccat tcataaagtt aaatatagat ttgcgaactt
     5521 cagaattagt caatccttcc aaattcacta taactgccct tctttgtttt agatggtctg
     5581 ctatagttgc agcttcatca taaaccttag gttcaactat tacaactttc atttggctag
     5641 ttgtatgtaa atttacaatt ttatttgctt ttgcagtact aaagccactg ttcatttctt
     5701 cttcttgaac tatatcgtcc atgccacttt catattcatc ttcaatgtaa tcttcttctt
     5761 catcaactat ccaattctta aatttagata ttattccatt tgacattatt aacttcctcc
     5821 ttaaaattta ttcaaatttg agtaattatt ttttattgta gtttctttct ccaaagattg
     5881 atgttccaac tctaacaatg gtagcccctt cctctatagc aatttcataa tcgtgactca
     5941 ttcccattga caaaacatcc atccctacat tagggatgtt tagttctttt atttgttttg
     6001 ataaatcttt aagaccttta aatacacctc ttatctcatc ttcatcttct ataaaaggtg
     6061 ccattgtcat tagacccttt atttttatat ttttatattt ttctgacact tctttgacaa
     6121 agtctacaac ttcttctcta tcaagtccat gcttagactc ttctttagat atattcactt
     6181 gtactaagca atctatttct ctgtttattt gttctgcacg tttttgaatt tcttcgcata
     6241 atgcaattct atctaaagag tgtatcatac aaactttatc gattatgtac ttaactttat
     6301 tggtttgcaa agttcctatc aggtgccagt ttaatttatc acctataata tcatactttc
     6361 tagctaattc ttggggttta ttttcaccaa cagaagtgat tccttccctc attgcctcat
     6421 taactaagtc tacatcaaca gtctttgtga ctgccaaaag ggtaatctct ttgctgtctc
     6481 tatctacttt ttttgcagct ttatcaattt tgtttcttat ctcagacaaa ttatctttaa
     6541 tagaacccat tattatcacc tactttatcc ttatatttgt gtttatactg ttaggtctta
     6601 aaattatttt gtcatataga ttaacacttg taactccatt tatgtcattt ttataataat
     6661 ccacaaatac agaagattca ttctcataag ttattccttc taatatgaca aattttacac
     6721 tttgtgattg ttcatcaatt acatatacac cacgtttatt gtcttttgtt tttatagaag
     6781 ttttaggtat ttttaaacat tccatctgat tatatattat atcaaattct tcgacacttg
     6841 tatcctgatt tgcaacaatt tggttactta tttttaattt taccattatt ttattattat
     6901 tttcatatat tttgtaaact tttgcatcta atttttgctc atgatattca attattacat
     6961 tttgattttt ttcaaagtta ttagaatctt ctttactgat gtatgtagat agataattac
     7021 tctggtcatt ggcatctttt tctatagttc cttttacatg aatctttgca tcaacttttt
     7081 cattttttaa caccaacgta tctacatttt tgctaattat cattactaat atattaaata
     7141 ctaaatatat aagaatacca agtatcacta atctggtata ttttaccttt ttcaaagact
     7201 ctccccctat aaaattactt tcattataag tacttcttct aaaaaatgtt tattcctttt
     7261 ttataagcta atattttacg ttctaataat tattttttcc atattaaatt tattttttat
     7321 caatcttcct atacaagact gcattattta tatataattg tattataaag tataagttta
     7381 atttacattt tatacgttaa gcttacttta tatgttttta tagttatttt tatttttaga
     7441 tgcaatataa aaccaattaa gatttaaaat aacttttgtt atgttgacat ttatttaggt
     7501 aatcatttat gaatcaggat aacgttatca taacaaaagt ttatgtccta aaaaaaatac
     7561 aaggaggatt ttcttatgtc agaaatttta ttaggaacag gtttactttt attggttcta
     7621 tgttgtttca caatttttag ttacaaggcc ccatatggta tgaaggctat gggagctcta
     7681 gcaaacgctg catgtgctac tttcttagta gaggcattca acctttcttt acttggtgat
     7741 gttttaggta tggaattttt tagaagtgtt ggagctgcta atggtagtct tggtggagtt
     7801 gctgctgcta tacttgtgcc tttagcctta ggtgtatctc cagtttatgc agttttaata
     7861 ggattatctt gtttagattt ttctatatta tctggattcg ttgctggata ctttgtttct
     7921 ttcttaatta agtttataga gaaaaaagtg ccacctggag tggatttagt ggttgtaatt
     7981 cttctatgtg caccactatc tcgtcttata gcatcaaatg tagacccatt agttaatagt
     8041 acactactac aaattggaca agttttgata gaagctacca acattagccc tataataatg
     8101 ggaattatac taggaggtct aataacagta gttggaacgt ctccacttag ttcaatggct
     8161 cttacatcta taattggtct tactggtctt cctatggcaa taggagcatt aggtgctttt
     8221 ggttcagctt ttatgaactc aacactattt gcaaaattga aattaggttc taaaaaagat
     8281 attatatctg ttgctattga accattaacc caatcagaca taatttcagc taatccaata
     8341 cctatatatt ctactaattt ctttggagga gccttttctg gtatcattgt agccttgatg
     8401 ggtcttgtaa acatgacacc aggtacagct acacctgtgg ctggttttgc tataatgttt
     8461 gcttataatc cagttcaaca agttcttata acagctggtt tatgtgcttt attcaatgta
     8521 attgcaggat ttttaggttc aaaaatattc agtaccttta aagttcttac tgctggtgaa
     8581 atacgtggta ctgatgtaga agaagaaatt gcttaaaaaa caataagatt tataaattta
     8641 tatctcaagt agtagagttt tatattgtag gcaattttat taattgcaaa cgttttattt
     8701 tttatttatg tttatgatta ttatgtgtgt tgaatattta gataaatttt tatttattat
     8761 ttttatttat tatttttatt tagatttttg tttagttttt tatttggttg atatattagg
     8821 aataaaaatt agtatagttt attactagtt agatagcata actttttaga tacataaaaa
     8881 attttatatt aattagatat tattaaaata aaagcgatac atgaattttt aaatgtccat
     8941 gtatcgcttt atttttgcca taatcttaac aactaatttt taaagtttat tatcttaaca
     9001 aagttcatta tcttatataa tatttaaatg catagacttc tttaatttaa ccatctaaat
     9061 atttaattaa atattattat atgtaatata actaattttt aaatgaatgt ataggagctg
     9121 gaatccttcc tcctctttga gtaaataatt cagatgaatt tgtatttata ggcattactg
     9181 gtgctcgtcc taaaagtcct ccaaattcta ccatatcacc tacatcacat cctggggctg
     9241 gtataattct aacggctgta gtcttattat taatcatacc tattgctgcc tcatcagcta
     9301 tcatggctcc aagcgtacct gctgtagtat ttcctggcac agctatcata tcaagaccta
     9361 cagaacaaat agcagtcatt gcttcaagct tatctatact taaagcacct ttaataacag
     9421 catcaatcat acctgcatct tcagatactg gtataaacgc tccactaagt cctccaacat
     9481 gtgaacatgc cattactcca ccttttttaa cagcatcatt taaaagtgct aatgctgctg
     9541 tagtcccatg agtaccaaca acttcaagac ccatttcctc taatatattt gctactgaat
     9601 caccaatagc aggtgtagga gctagtgata agtctattat cccaaatgga acatccaatc
     9661 tactagaagc ttcctttgcc acaagttgtc caactcttgt aattttaaat gcagtctttt
     9721 taactgtttc tgaaacaaca tcaaaaggct ctccctttac tttctctaaa gctctcttaa
     9781 caacaccagg gccacttaca ccaacattta taattctatc agcctcacca acaccatgga
     9841 aagctcctgc cataaatgga ttatcttcta ctgcattaca aaatactact aattttgcac
     9901 aaccaaaacc ttttgcatct tttgttaatt ctgcagtttc ttttatgatt tctcccattt
     9961 ctttcactgc atccatattt ataccacatc ttgtagaacc tacatttact gaagcacata
    10021 ctttattagt acttgctaga gcttttggaa ttgacttaat tagtatctta tcaccttttg
    10081 tatatccctt ttgaactaat gctgagaatc caccaataaa atctatacct aaatcctctg
    10141 cagccttatc caatgtctgt gcaaacttta catagtcatt ttcatctgtt gcacctgcaa
    10201 taatggaaat tggagttact gatacccttt tatttacaat tgggatacca aattcacgct
    10261 ctatatcttt tccaaccttc accaaatctt tagcagaact cataatctta ttgtatattt
    10321 tttctctagc tttatctcca tcagggtcta tacaatctaa taaagaaatc cccatagtta
    10381 tagttcttat atccaactgt tcttcatcta tcatctttat tgtttcaagt atatttccta
    10441 cattcactat tatcacctcc ccttactgtc tactacagat tatgcattga attaaatatt
    10501 tcttctcgct gaatttttac atccactgat aatttcttac ctctttcaac caagtcctca
    10561 accgttttct caaaagaatt taatgatttc tccaaatcaa ctatcataat catagtaaaa
    10621 taatcctgta atattttttg tgatatatct actatgttga tattttgctt gtataattca
    10681 tcactaattc ctgccactat acctggttta tcctttccaa ttactgttaa aattgctttc
    10741 ataataatat cctcccttat ttttttataa atcttatatt tttacaaatt gaactatgtg
    10801 ttaacatata tttcatttat ttgttagtaa accacttttt atgtatatat ttaatatatt
    10861 ttaataccta tacattcatt tgtcaatttt ttttcaatat tctat
//
LOCUS       DAELJO010000133        10736 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELJO010000133 DAELJO010000000
VERSION     DAELJO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.133"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            283..741
                     /locus_tag="KPB88_002723"
     CDS             283..741
                     /locus_tag="KPB88_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0054990.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHDKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            802..1077
                     /locus_tag="KPB88_002724"
     CDS             802..1077
                     /locus_tag="KPB88_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0054991.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1136..2410
                     /locus_tag="KPB88_002725"
     CDS             1136..2410
                     /locus_tag="KPB88_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0054992.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2540..3592
                     /locus_tag="KPB88_002726"
     CDS             2540..3592
                     /locus_tag="KPB88_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0054993.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DNHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3592..4623
                     /locus_tag="KPB88_002727"
     CDS             3592..4623
                     /locus_tag="KPB88_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG0054994.1"
                     /translation="MKAVVKTNPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4715..5527
                     /locus_tag="KPB88_002728"
     CDS             4715..5527
                     /locus_tag="KPB88_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0054995.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5530..6450
                     /locus_tag="KPB88_002729"
     CDS             5530..6450
                     /locus_tag="KPB88_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0054996.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NVSTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6625..7071
                     /gene="rpiB"
                     /locus_tag="KPB88_002730"
     CDS             6625..7071
                     /gene="rpiB"
                     /locus_tag="KPB88_002730"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0054997.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7101..7766
                     /locus_tag="KPB88_002731"
     CDS             7101..7766
                     /locus_tag="KPB88_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0054998.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTD
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7876..9378
                     /locus_tag="KPB88_002732"
     CDS             7876..9378
                     /locus_tag="KPB88_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG0054999.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAKKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9690..>10736)
                     /locus_tag="KPB88_002733"
     CDS             complement(9690..>10736)
                     /locus_tag="KPB88_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055000.1"
                     /translation="LLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEA
                     IAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEIST
                     LAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDR
                     ETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDK
                     IQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENI
                     ILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIID
                     ADLFKVMLTFRRVN"
BASE COUNT         4283 a         1220 c         1969 g         3264 t
ORIGIN      
        1 aaaaaccatt tgtaaaaaac aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata
       61 taaaaaagta tggctagtta agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa
      121 atttatcaat gaaagttagc taaaatcctg gaagagagca ttatttttgg gaaaacattt
      181 tcttttgttt cacaaaaaaa gccacttttt ggcacacaac atgctttata taaagtcaag
      241 aaaaactaag gtataagaca aaggcaatta ggaggtgtaa atatggagtt tataaatttt
      301 gtaagtgata aagttataga ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa
      361 gaaatacatg ataaagtttt tgatttaggc tttgtaaaag aagaatttgg agaaaaaata
      421 ttagcaagag aaaatgtatt tccaacaggt ttaaatttag gtgattatgg agtagcaata
      481 ccacatacag atgcagaata tattaaagag caatttatat cagtctgtac ttttaatgaa
      541 ccagtagtat ttagttctat ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc
      601 gtattaggac ttaatcaacc acacagtcaa ttatctgtat taacagaatt aatgggaatt
      661 atgcaaaata aagagttagt tgataagttg atgagttcta ctgataaaga agaagtttta
      721 aagacactaa agtctttata aaaataaaaa agtactaaaa ataaaaaata taaaaataaa
      781 aattattagg aggaaataaa aatgaaaaaa atattagtag catgtggagc aggaatagca
      841 acatcaacag tagtttgtga caaggtagag agattagtta aggaaaataa tatacaagca
      901 gaagttatac aatgcaaaat agctgaatgt gcaacaaagc aagaaggagc agatttaata
      961 gtatcaacaa ctatactacc aacaacttat aatataccag caataaaagc aacagcatat
     1021 ataactggag taaatacagc agcattagat aaaaaaatat tagatgcttt aaaataatcc
     1081 tatcatagat acattgaaat aatattttat aaaacagaaa aaggaggatt tataaatgga
     1141 taaattatta gcagtagtac aatacatttt aaatatggga ccgacagtaa tactaccaat
     1201 aacaattgta gtactaggaa tgatatttag aattaagttt acaaaagctt taaagtcagg
     1261 tcttacaatt ggtataggat ttgtgggtat aaacttagtt gtaaacctat taacatcaac
     1321 actagggcca gcggctcaag gaatggttga aagattcgga cttaatttaa cagtaataga
     1381 tgttggttgg ccaacaacag catcagcaac atgggcttca ccagttgcac caatattaat
     1441 accagtttgt ttattagtaa acttactatt attatacttc aataaaacaa agatattaaa
     1501 cgttgatata tggaattatt ggcactttat agtagcaggt gcagttggat atatagtaac
     1561 aggaagttat gtttgggcta tattctgtgc aatactaatg gagttattag tactgtttat
     1621 tgcagaaaag acagcaaaaa atgttcagga gttttatgat ttagaaggta tagcattacc
     1681 aactggttca acagcttcat ttgctccact tggatattta gtaggtaaat ttgttgaaaa
     1741 aataccagga ataaataaaa tacaagcaga cccagactct atacaaaaaa gatttggtat
     1801 atttggagaa ccaatgatga tggggattat actaggtata atacttggag cacttgcggg
     1861 atatgatgta gcaggaatat tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat
     1921 gcctagaatg gttagaatat taatggaagg tttaattcca atttcagaat caataagaga
     1981 tttcttacaa gcaaaatatg gagacagaga actttatata ggtctggatg cagcagtagc
     2041 aactggacat ccagcagtta tatcaacagc tcttatactt gttcctataa ctttgttctt
     2101 agcagttata ctaccaggaa acaaagtttt accatttgga gacttagcga caataccatt
     2161 ttatatggca tttatagtgt gctttagaaa aggtaatata gttcaatcag ttataacagg
     2221 aacaatagta atagctatat cactttatat ggctacaaac tttgcagatg ttcatacaca
     2281 attattacaa caagcaaata tgatgccaaa aggaacaaca atggtcacaa gtatagatac
     2341 aggtggaaac ttgctaaaat ggggaatatt gaaattatct caattagttt cttcatttgg
     2401 aatattctaa tatgtagtta atagagatta ctctttttta gtaaagtatg cgaaaattta
     2461 aaatttagca gctactaaaa aagagtaata ttttttataa atttttaaaa atacaaaata
     2521 caagaggagg agatttatcg tgaaagcagc agtattacat ggcacaaatg atatgagatt
     2581 tgaagatata gaaataaaac catgtgaaag tgatgaagta aaaataaaag ttatggcagc
     2641 aggaatatgt gggtcagatc ctccaagagt attaaaacat tggaaatatc cagttccagc
     2701 tataccagga catgagtttt ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt
     2761 aaaagttgga gatagagtag ttgcaattcc ttttatacct tgtaatgaat gtgaatattg
     2821 taaaagagga ttattttcat tgtgtgataa ccatggaatg ttaggagcta aaagttttgg
     2881 agcttttgca gagtatgtaa atataaaagc tacaaatgta cttccaatag gagatatgga
     2941 ctttgaagat gctgcaatga tagagccact agcagttgct atgcatggag tacttaatat
     3001 aggtgttcaa gttggagaca ctgtagccgt aatgggtagt ggaacaatgg ggcagttggt
     3061 aatacaagga cttaaaatag caggggcagg aacaattata gcagttgata tatctgataa
     3121 taaattaaga gaatcaaagg aattaggggc tgatataata attaatgcta aagatattaa
     3181 cccagtagaa aaaataaaag aacttactgg tggaaaaggc gttgacatag ctcttgaatg
     3241 tgcaggttca aaaataactc aagaacaatg tttacttata actaaaaaga aaagtaagat
     3301 aggattttta gggatagcat attcagatat aactttatca gaagaagcat ttgaaaatat
     3361 atttagaaaa gaactagaat taaaaggttt ttggaactct tattctgcac catttccagg
     3421 acaagaatgg actaagggaa tcaatctagt taatgaagga aagataaaat taaaagaaat
     3481 ggtatctcat agattctcac ttgaagatac ttacaaagca tttgaaatga taagagatag
     3541 aaaagaagaa tttaacaaaa tattaatttt accacaaggg gttgaaaaat aatgaaagca
     3601 gtagttaaaa ctaatccagg ctatgataat gtagaagttt tagaagtaga agaaccaaaa
     3661 gctacaggag acaaggttaa aataaaagta gaatatagtg gtatatgtgg ttcagatata
     3721 cattcattta aaggtgaata tgcaaatata aaagccccag taacattagg tcatgaattt
     3781 tctggaattg ttgtagaagt aggagaagat gttaaaaata taaaagtagg agatagagtt
     3841 acatcagaga ctacttttgc aacttgtgag aaatgtatat attgtgaaac taaagattat
     3901 aacttatgcc caactagaaa aggtattggt actcaagtaa atggaagttt tgctgagtat
     3961 gtattatcaa gagaagaaag catacatgta cttcctgaaa atgtatcttt attatcagca
     4021 gcccttacag agccactagc ttgttgtgtt catgcagctc ttgaaaagac tactatagaa
     4081 agttctgata cagttttaat aataggacca ggaccaattg gtttattatt agctcaagtt
     4141 gttaaatctc aaggtgctac agttataatg tctggtgtta caaaagataa ggaaagatta
     4201 gaaatagcta aaaatcttgg agtagataga actgtaaatg taatggaaga aagtttggca
     4261 gatgtagtga atgaaatgac tgatggaaat ggagtaaaca aaggatttga ttgttctggg
     4321 tttatgccag cagttaacga agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa
     4381 gttggaatct ttgctaaaaa attaaatgaa atggaccaag aagcaataat ccaaagagag
     4441 ttacaatata taggaagcag gtctcaaaag ccaagttctt ggattacagc attagattta
     4501 atggcaaatg ggaaagtaaa tacagaagct ttagttacta gaactgttgg tttagacgaa
     4561 tttaaagaag gtattacagc tttaatgaac ggggaagaaa taaaagtagc agtaaaatct
     4621 taaaaaaaca tttgtattat tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat
     4681 gtaaggtctt aaaaaataac aggaggaaga aagtatgagc actcttagag atcattctaa
     4741 taatattaga aaaaacataa taaaaatggt tgcagaagct aaaagcggac atccaggtgg
     4801 ttcattatca atagctgata tgttgacagt attatatttt gataagatga acgtatgtgc
     4861 agaaaatcca aaaatggctg atagagatag atttgttcta tcaaaaggtc atgcagcacc
     4921 agctttatat gctacattag cagaaaaagg atttttccca gaagaagaat taataacact
     4981 aagaaaattt ggttctaagt tacaaggtca cccagacatg aaaaaagtag caggaatcga
     5041 tatgtctact ggttctcttg gtcaagggct atctgcagca aatggtatgg cacttgcagg
     5101 aaaattagat aacaaagact atacagtata tactatatta ggtgatggag aaatccaaga
     5161 aggtcaaata tgggaagctg caatgacagc atcacattac aaattagata atttaattgc
     5221 atttgttgac ttaaatggat tacaaataga tggttctaat gaagaagtaa tgaatgtaag
     5281 tccagtagat gacaaattca aatcttttgg atggaatgta atagtaataa atggacattc
     5341 ttttgaagaa ataggaaatg cagttgatga agctaaaaaa gttacaggaa aaccaactgt
     5401 aataatagct aaaacagtaa aaggtaaagg tgtttcattt atggaaaata atgctgcatg
     5461 gcatggaaca gctccaaatg cagaacaagc agagcaagca ttgaaagatt tagaaggagg
     5521 tctataggaa tgggaatagc aactagagaa gcttacggac aagtgttaaa agaacttgca
     5581 gaaaataaag atatagtagt attagatgca gatttaggaa aagctacaaa aagtatatca
     5641 tttaaagaag ttgccccaga tagatttttt gacatgggga tagcagaagg tgacatgatt
     5701 ggtacagcag caggtcttgc tacttgtgga aaaatacctt ttgcgagtac ttttgcaata
     5761 tttgcagcag gaagaggcta tgagcaaata agaaactctg tagcatatcc aaatttaaat
     5821 gtaaaaatag cagctactca tgcaggtgta acagtaggtg aagatggtgg tagccatcaa
     5881 gctatagagg atatatctct aatgagaggt ataccaaata tggttgtatt aaatccagca
     5941 gatgctttag aagcaagaca agctattctt gcatctattg attataatgg acctgtttat
     6001 ataagattag gtagagctgc aactcctgat gtaaacagtg aaaattataa atttgaaata
     6061 ggaaaaggaa ctgttttaag agaaggttct gacataacag taatagctac aggaataatg
     6121 gtagcaaaag ccttagaagc agcagaagaa cttgctaaag aaggtataaa tgtagaagtt
     6181 gttaatgtat ctacaataaa accattagat gaagcattaa taaaagaaac tgctaagaaa
     6241 actggaaaag tagttacagc agaggagcac agtataatag gtggtttagg ttcagctgta
     6301 tgtgaagctt tagcagaaac taaagatgta gtagttagaa gaattggtgt taaagatgta
     6361 tttggtcaat caggtacacc agctgattta ttaaaacact atggtttaac tacagaagat
     6421 atagtaaaaa atataaaaga gttgttatag atataaatat atctaaataa caaattgatt
     6481 tgacataagt caattaaagc tgtcttaaaa atagaaagaa atttctacaa tgggacagct
     6541 ttatttttac aatactttat ttttatgata attataaaat agtctgaaag taatcaaaaa
     6601 aattataaaa agaggtgcta tttaatgaaa atatctattg gatgtgacca tggtggttat
     6661 gaattaaagg aatatataaa ggaaaattta ataaataaag gtatagaagt tgaagatgtg
     6721 ggtacaaatt caaaagaaag tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat
     6781 gttcaaagta aaaaatctga tttaggcata ctttgttgtg gaacaggaat aggtatgtct
     6841 attgcagcaa ataagtttaa aggaatcaga gcagctgttg tttctgactg tttttcagct
     6901 aaggcaacta gagaacacaa taatacaaat gtactttgtt taggagaaag agttattggg
     6961 aaaggattag caatgagaat tgttgaagaa tggttaaatt caagttataa tggagaaaga
     7021 catgcaagaa gattacaaat gattgatgat atagaaaaag aaaatttata aatatataat
     7081 ttataacaag ggggagcgtt atgtctatta tttgtgcttc aataatgtgt gcagaccaaa
     7141 tgaagttaaa agaggaatta gaagctcttg aagaagcagg tgtaaaatta cttcattgtg
     7201 atgttatgga tggaatattt gttaaaaact tggctatggg accagagtta ttaaaatcaa
     7261 taagtgaaaa tacaacaata cctttagaca tacacttagc cacagaaact ccagataaat
     7321 atattgatat gatgtcttat ataaagccaa aatatatttc ttttcatgta gaatcaagta
     7381 caaatgtaaa agctgatata caaaagttaa gaaattatgg tatagggcca gtgttagcaa
     7441 taagccctca aacaagtgtg gataaaatag aagaatatat aagtttagtt gagggtatct
     7501 tagtaatgac agtaaatcca gggtttgcag gtcaaaagtt taatctttct gtattggata
     7561 agttggataa acttacagat atactaaaag attatgataa tcctccattt atagaggttg
     7621 atggaaatat aaataaggat actattaaac ttatgaatgg taaaaaagta gatatatatg
     7681 ttgttggtac ttcagcttta tttaatgata agcctccaat ttcatataaa gataaaatag
     7741 aagaactaaa agaaagtata aagtaaccat gtaataaaat tataatatgt ttctgtttaa
     7801 tagtaaaaat ttaggtggaa acaagaaatt ttataatatt taataaaaat tttggttgag
     7861 taaggagaat tttttatggg agacctatat aaactacaaa atggtactga tattagagga
     7921 atagcgtatg aaaatgattc taaagaagtt aacctaactg tagaagaagt aaaaaaaata
     7981 gctaaagcgt ttcatatttg gttaaaagaa aagactaaga aagataaagt aactgttgcc
     8041 ataggaactg attctaggat aacaggaagc caatttagaa gtactgttat tgaaacttta
     8101 actaatgacg attgtaatgt aattgattgt gagattgcca caactccagc tatgtttatg
     8161 acaactatta tggatggata caattctgat ggggcgatta tgattacagc aagtcactta
     8221 ccttattact ataatggttt aaagtttttc acagaaagtg gaggtttaga aaaaactgat
     8281 ataaaagaga tgttggatat agctgttaaa aatgatagtg tagactatga ggaagtaaat
     8341 aaaaaaggtg aagttatagt aaaaaattta atagaagatt attctaattt attgatagat
     8401 aaaattagaa aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc tgggcttaag
     8461 atattggttg atgcaggaaa tggggctggt ggattctttg ctaaaaaagt tttacacata
     8521 ctgggagcag atacgacagg tagtcagttt ttgaacccag atggtatgtt cccaaatcat
     8581 atacctaatc ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt gttggataat
     8641 aagagcgacc taggaataat ctttgataca gatgtagaca gagctgctat tgtaggtaaa
     8701 aatggcaaac caataaataa aaatgctctt atagctgtta tatcatcaat agtattagaa
     8761 gaacatccaa aaacagctat agtaacagat tcgattactt ctgaaggact tgctaaattt
     8821 ataaatgaat tacaaggaag acatcataga tttaaaagag gatataaaaa tgtaataaat
     8881 gaagctataa gattgaatag tgaaggtgaa gagtgccatt tagctataga aacgtctgga
     8941 catgctgctt taaaagaaaa ttatttctta gatgatgggg catatttaat agctaaaatt
     9001 ttaataaaag tagcaaagct atccttggaa ggaaagacta tagaagaatt gatagaaaac
     9061 cttgaagaag ctcaagaaga gaaagaagtt agaataggca ttaataaaga agattttaga
     9121 ccatatgcag aggatatatt aaaagattta gattctcatg tcaagagtat tgatggatgg
     9181 tctgtagcac caaataattt tgaaggcata agagttaatt gtgatgaatc aaatggagat
     9241 ggatggtttt tacttagaat atcactacat gaaccattat tagctttaaa tattgaatca
     9301 aataaaattg gcggagcaga acaaatttat tctaagttaa aacttttctt agaaaaatat
     9361 gatttaaaag gtctttaggg taaatagaaa gaatatattt atattgaaga ataaatgtaa
     9421 atttgtacct aagattacaa atattacaaa attgtaatct taggtaacag agatgtaact
     9481 gattatggaa acactatgtg atattataga tacagagaga tgaaatatgt gatgaattat
     9541 agagaaatat aatttaaaat gtattaatat cacaccaaaa aggtataatc aaaaaatatg
     9601 taaatataaa ccccaacatt tagaaaaaga cctctaagga ggtttttttc ttgtttaaat
     9661 aacttaaaat atatttttat attaatatat caattaactc ttctaaaagt aagcattacc
     9721 ttaaataaat cagcatctat aattatttcc atgtgtgcat tttctaattc aattatattt
     9781 tgagcaattg ataaaccaag accagagcca tctgtttttc tagatatatc accccttcta
     9841 aatctttcta ttaactcttc tggctttaga tttagtttat catttgaaat atttttaaac
     9901 gttaatatta tattttcttc ctttataaac atatctatat agaccctagt gttaggcata
     9961 gagtatttta gtatattgca aattagattc tcaaaaactc tccaagtttt tttaccatct
    10021 atcatggtaa aaacagcata ttcaggtgtt tctattataa aatctaaact gctattttga
    10081 attttgtctt caaattctac aatactttga attaaaagtt ctcttatatc ggtttttgtt
    10141 atattaaatt ctacatctcc actacttagc ttagatgctt caaatatgtc atctattaat
    10201 aattttaatc tttgtgattt tttatctaaa atatttatat agtcatttat agtctctctg
    10261 tcaaagtcat tattttttaa catatcaaca tagcttaata tagatgtaag tggagttttt
    10321 aagtcatgag atacgtttgt gataagctct gccttcatcc tttcattttt tatattagca
    10381 tctacagcag tttcaagacc gcctgttata ttatttatac ttttggctaa agtagatatt
    10441 tcatcatctc ctataacttc taatttatac tttaaattgc cattttctat attttttata
    10501 ttttttgata tgttatttat gtaattaaac ttaacagata aaaagttgta tatataaaaa
    10561 tgaaaaactg tgtttacaag tgtaaataaa attactatag ctattgcctc agcataatat
    10621 atatacataa agaatatcaa aacaagatat tggataatag cttgtacaac tatagatatt
    10681 aaaaatacta tagctaattt tttatttata gtttttttat tatacatact aagtaa
//
LOCUS       DAELJO010000134        10716 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELJO010000134 DAELJO010000000
VERSION     DAELJO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.134"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            29..904
                     /locus_tag="KPB88_002734"
     CDS             29..904
                     /locus_tag="KPB88_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG0055001.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1263..2567
                     /locus_tag="KPB88_002735"
     CDS             1263..2567
                     /locus_tag="KPB88_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG0055002.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            2751..4142
                     /locus_tag="KPB88_002736"
     CDS             2751..4142
                     /locus_tag="KPB88_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG0055003.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4319..4906
                     /locus_tag="KPB88_002737"
     CDS             4319..4906
                     /locus_tag="KPB88_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055004.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            4939..5730
                     /locus_tag="KPB88_002738"
     CDS             4939..5730
                     /locus_tag="KPB88_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0055005.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5911..8328)
                     /locus_tag="KPB88_002739"
     CDS             complement(5911..8328)
                     /locus_tag="KPB88_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055006.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8341..9006)
                     /locus_tag="KPB88_002740"
     CDS             complement(8341..9006)
                     /locus_tag="KPB88_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055007.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9088..10119)
                     /locus_tag="KPB88_002741"
     CDS             complement(9088..10119)
                     /locus_tag="KPB88_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055008.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10109..>10716)
                     /locus_tag="KPB88_002742"
     CDS             complement(10109..>10716)
                     /locus_tag="KPB88_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055009.1"
                     /translation="ITSKFTAKSALKSIFECEFDLIILDINLPDKSGFELCNEIKENY
                     NIPIIFLTANDMECDMIKGYELGALDYITKPFNINIFKQKVKAFLNHLTVKTKQDYYR
                     DGYLEINFSELSANINGNQIIFTPLEYRTLKLLTENPKSILTRKVLLEKLWDIDANFV
                     DEHTLTSVISRIRSKIEKDNLKYIKTVYGMGYMWLGERNEF"
BASE COUNT         3916 a         1308 c         1587 g         3905 t
ORIGIN      
        1 atgaaactaa tggggaggtt aaaactcaat gattactttt gatgtaaatg gagctattat
       61 cagatttgat gatacaaaaa ataattataa taagataagg aaaatcttta aattatatgc
      121 tttggaaata tctaaaaact ttgagagaga ttgtttaaat agttttcaaa atctaaagca
      181 aatatctgat agaggtttaa gtttaggaga aaaatatata gaagagtctt ttaagaaagg
      241 aattgagact atagtaagtt ttggaattat aactatagat atcaatacct ttaaaagtgt
      301 ttattgtgaa aaatatttag attttaagag actttttaat aacttgaata aaacttttat
      361 tataccaaat aaaaataaaa aaaatggcca cgcgagcttc ttagaaatag aatcgacaat
      421 aaagaaaatg agcaattatt tatacaatga ttgttttaaa attcattatg ctgttgtaga
      481 tgcccttata gaaaatcgta taactgcggt acattcttat attgatgaag aatctataaa
      541 aaaatctaat gccttattta ataattataa agatggtttt attgggaagg ctgacgaatg
      601 caaagtagta aaacaaataa taactttaaa tccatatagg gaagatgtat acgaattttt
      661 ggtaaaagaa gatggagatt ttagtaagga aattgagaga ttgactgaat ttttaggcta
      721 tgatataaga gaatataaag cgtcattaat ggatatttat ataaatgagt taatggagag
      781 cgatgaaaat gatgttgaaa atgcaaaaga aaagattgaa aaatatgcta gatatattgg
      841 atgtagtgat gagaatttat atgtagcaag agttaatgcc atatatactt ttgcaaatgc
      901 ataattttat taaacattaa atattagatg ttcgattata taaatttaaa taaaaaaata
      961 tccttattaa taacaaatag tttatattaa aaaccattct taaaatgagt ggttttttat
     1021 tttgaatata tatattctaa acctaatagc attcctatct ttaattagcc aaaaatcaac
     1081 tggatttcat atagactaca taattttaaa ttgctaaaaa agacaatatg ttgacaaaaa
     1141 tagaatatgt tgataaaatg aaatagtcaa tatggtgtct taatttaaaa atttatttat
     1201 gttgtattag ataaagctgt tttatataaa acataaataa ttattataag gagaaataga
     1261 ttatgaaagt aaatgtatac aatgtagaat catttgtgga tgaaagactt aagattatgg
     1321 tttcggactt aattccaaat actaaactta aaataagtgt gaaaatggaa tttccatggt
     1381 gcaagggaga agaattttca tcttatggag tgttttgttc aaatgaaaaa ggagaagtag
     1441 atttagattt agttgaacct atagaaggta cttataaggc tgataattgt atggggttaa
     1501 tttattcgct ggaaaaatct aaaactgaag gtaaaaattt tgcagaaaat atatctattg
     1561 ataaaccaat aattatgaat atgatatttg aaacttctat tgaaagaaaa gaagttgaat
     1621 taaagagaat atttaaatca gaagatatta ccacaaaata tatatctgat gagtttatag
     1681 gaaaattatt ttataaacaa aattctaata ataaaacaat tttaatgcta ggtggttctg
     1741 atggaaattt agacgctcta gaccttttag cagctccact tgcatctaga ggattcaatg
     1801 tactaacagt tgcatacttt gctttagaag ggttaccaga taaattagaa gaggttccat
     1861 tagaatattt tgaaaaagta tttgagtgga ttagtgaaaa tgaaattacc agtactaaag
     1921 aaatatttgt acatgggaca tcaaagggtg gagaattggc ctttttactt gcttcaagat
     1981 atgaacaaat taaaaaggtt gtagtatcac aacctcacat ttattgtttc caagctctta
     2041 atggattgat gagtggaaat gatacttcat catggtcata taaaggagag ccatttccat
     2101 atatcaaagt agacaatgat atattctttg aagaacagaa aaaaaatata tcaaaaggta
     2161 ttccatttgg atttaatagt acttataaga aaagtcttaa aagagcagag aatagaaaag
     2221 aggctagaat aaaaatagaa aattccaaag cagatatact tatgattgct ggaaaagaag
     2281 ataacatatg gaatagttat gaagcttgtt taaaggcatt agaaatcttt gaagaaaata
     2341 attatccata taacgtagaa ttattaacat acaataatat gggtcatccg ttaccaattc
     2401 catatattat gcctctaaag gagactttat gtatgagtat gagtggaggg attttttctt
     2461 caggtggaac tgtagaagga aattcaaaag gacagtttga atcatggaat agaactattg
     2521 aattttataa aaaaccttta tcaagtgatt taaataacta ctattaagtg atttaagtaa
     2581 ctaaaaatga agcggtcttt ctatatatgc acaaaatcta tcaattttat agatttaatt
     2641 tattgtttat ataatttgca tgttatatac taattttaat gacattgata atttataaac
     2701 aattataaag ttgtaaactt ataagagtta ttgaaaggtg gagtgaagtt atggaaagac
     2761 tcgctagaag ggaacaacct catataaaat ttggaatgtt taagataaga attccattta
     2821 tacattacag atttgagaaa ccagaagcta ttcaaggtat aatatcaagt atgacaagtt
     2881 taggcacaat tggccttagt acacaaattt tgggattacc ctatgaagtg gcatggagta
     2941 tggctatcat taactcaata ttatactgtt tgcatgtttt tatgggagac ccagttgtac
     3001 ctggctggat tacagcttct ataactctta caactgcata tttgttaaag ttcagcatgg
     3061 gcattgaaag aatacaagca ttaacagctt tacaaataga tttaggaata atatttattt
     3121 taatgggtat aactggtgta gctggaaagt tagttagtaa gataccaaat tctattaaag
     3181 gtggaatact aatgggtgtc ggtatatcga ctataatatc agagtttaat cctaaaggga
     3241 gatttgattc ttatcctata tctataacag taggaatttt ggttgcttgt tttgtaatgt
     3301 tttctgaaag atttgatact ctaaaagtta agaataaatt tttatttcat ttaggagaat
     3361 atggtgtagt atcagctatt ttagtgagtt taatagtagg tatatttagt aaagaaatag
     3421 aaattcctag atttagtttt gacaatttag tttacatagt agattttaaa aatctgataa
     3481 atacagtatc tccttttgga ataggttttc caagtgtaat gttatttata caagggatac
     3541 caatggcttt tatgatatac ataattgcct ttggtgattt tataacaggt gaaaacttag
     3601 tactttctga aagtgaaaat agaaaagatg aatatataga tttcaattca aataggtcta
     3661 atgttataag tgggataaga aatattttta tggcaatctt ttctccatat ataccaatgt
     3721 gcggtccatt agctgcgaca cttacaggaa gtgttgctca aaggtacaag gttggaaagg
     3781 aagccatgca gtctgttttt agtggcatgg gtacattagt atgggtttca gcaattttta
     3841 tctgttttta tccaattgcg cagattgcta cacctcttat accattagca ttgtcagtaa
     3901 cacttttagt tcaaggatat ttatgtacaa agctatcaat ggagctatgt aatacaggtg
     3961 ttgagaaggg attgattgga cttatgggag gtgtaattgc tgctaaaggc ggaacatggg
     4021 gacttgctgt aggttttata ctgtatttca ttttaattga ttctaaaaaa agaaaagaaa
     4081 aagatattaa ttcaatagaa gattgtgtat atgaaatatt agacaaaaag aagagtattt
     4141 aaaggaaaat ctgagttgct gttatctatt aatagattaa ttgcaactca tgttttattt
     4201 ataagtttta ggaattgcaa aaaaatgcaa agctttttct tgacataatt tcttgatatg
     4261 ttactatagt tatataacac atgtgtttta taaaaattta atggagaggt gaacatatat
     4321 gccacctaaa gtaaagataa caaaagaaat gattttgaat acagtcttgg atattacaag
     4381 agaagtggga tttgaagctg taaatgcgag gagtattgca agtaaattac agtgttccac
     4441 tcgaccaatt tttacttgtt atgaaaacat gaatgaatta aaaaatgaat ttcttgcttt
     4501 tgcatatgaa tattataatc agtatgttgt taattatcgt aattctaaaa gtgtaagccc
     4561 ttatttactg cttccgcttt catacattga atttgctcaa gaagaaacgt atttatttaa
     4621 gttgttattt ataaatgatt tagatttgat gatgaaagag gcgaatgatt tttataaaga
     4681 aattgacaat gaaaaaaagg caagactttt ttcggaaagc attggaatag atttggaatg
     4741 tgcaaaagta atatttttag atttatttct ttatactcat ggcatagctg tcttaacagc
     4801 aacaaaaaaa ttaacattag atagatatag tgctgaaaaa atgttgaaaa atatattaac
     4861 agcttttata agacaagaaa aatcagattg gaatttgtct atttgatgtt tattaactaa
     4921 tatagaagag gtgaaattat ggaaacaaaa caatacttaa ttgactttta taatacttat
     4981 gatgaagata gtcgacttgc attaaaacat ggaatggtgg aatttctcac tactatgcat
     5041 tacattgata agtacataaa atcaggtgat tgtgtgcttg aaattggcgc tgcaactggt
     5101 cgttattctc acacactggc acgtcaggga tatgatgttg acgcagtgga attagtcgaa
     5161 cataatatag aggttttcca taaaaatact caatcaaatg aaaatataag cattactcag
     5221 ggtaatgcta tggatttatc tgttttccca gataataaat atgatattac gttactgtta
     5281 ggaccactat atcatcttta taacaaagaa gataaacaac aagctttaca tgaggcaatt
     5341 cgtgtaacaa aacctggtgg tgtggttttt gctgcatatg ttatatctga tggctgtctt
     5401 attgacgagg ggttccatag aggaaatatt aatgtatctg aatatattga aaaaggtttg
     5461 attgatccac agacatttgc tgctaagtct gaaccaaagg atttgtttga gcttgtacgt
     5521 aaagaaaaca ttgatgattt gatgtctgca tttaatgtaa ctagactgca ttatgtcgca
     5581 tcagatggtt tggctttgta tatgcgtgaa gcagtagact ctatggatga tgatgcattt
     5641 gcattatatt taaaatatca ccttgctact tgtgaacgtg aagatttagt aggggttaca
     5701 agtcatgcaa ttgacatatt tagaaaataa ggaatatgct tgtttatgtc tctggtgatg
     5761 ttgctcggag atataagtaa gtgtatctgt ataaaataaa aacaagtata gggatagaaa
     5821 attatgtaaa gtcaaaattt tctatccttt tagcttttaa aaaattatgt taaaacttgt
     5881 ataaaaaatg gctatttttt atatacattc ttactgagaa cgtaagcgtt ctgtaagtga
     5941 ttttttttct atatttctta caagtaattc agtcactaaa acttgtatac caattaatac
     6001 gaatgaaaaa attaaaatag taaatataga aaatttaaaa ttaataaagt ctaatctaat
     6061 tacatctttt ataaaagtgc aaagtagatt tgaaccaaaa taacctagta tacttgaagt
     6121 acatatagct ttgatactta tgtttagacc ttctctataa agcatatttc gcagttgctt
     6181 tcttgtcatt ccaacggctt gaagtaatgc aaactctttt tttctaatta aaacgctgtt
     6241 tattagggta ttggtcatat ttagtatacc aaagcaagaa attaaaccaa caaaaatgta
     6301 tgaagctata attatatatt gaaatgctct agtaatcatt ctataatcat cttcatatga
     6361 ataaagcatt aaatttgaat ttttctgaat tatacttaac aacttttctt caacttcttt
     6421 agaatgcttt ttatctatta aaatagaaaa atctaaattt gtattatatg gtgttagaga
     6481 ttctaaagtt ttgtctgaag caaaaaacat acctcctgta ttggaattgt caatgattcc
     6541 tataattttc attttctttt taataatatc atttccacta aagatattaa atgtaactgt
     6601 atctccaact tttaaattta atccataata ttttgttcta tatttattta taattaaact
     6661 atctgaatct aaatgtgtat agtctatttt tccactgaca atatattctt caagtgattt
     6721 tgaaagttca ggcgtaatac ctttaattag agtaataaat tgtttttcgt ctctatcttt
     6781 gatgtaaggt tctataatat tacttttaat tgatttggat tcaattaatt tttttactcc
     6841 atgaatagat ttaatttcat tcttaagaga atctgttagt ggattatttt gttggatttc
     6901 tgtaatatgc tcatataagt tatcactctt aatgctaatt atataatctt cgttatagac
     6961 aaattgttga gatagttgtt ttttgaaatc tattgaatta gcaagattag cgattgaaat
     7021 aaataaaact ccactaatgc ttagcgatat taatgcaatc aaatttttct ttctatttga
     7081 ctcgatattc tttaatgcta gattttttat atttatataa ctaaaatttt ttgctttttt
     7141 tctaaattta gtttctgtat attgcatggc ttcaatagca gatattttag aaataattct
     7201 agctggttta aataaagaaa ttctaacagt taaaaacatt accagtgata aaattaaaat
     7261 tataaataaa tcagctatca tttgccatgc taatggttga attatgtaag caattataca
     7321 agaagctata acccctaatg gaataaaaat aattgacaaa atatttcctt gtagagaaat
     7381 aatctttttt aactgaaaag aagtcatacc taacgatttc aacttagcat acatctgaac
     7441 cgaattaaca actgaaatat aaaaaatact atatataaca aaaacgctag aaaaaagaac
     7501 aactattcca actactaaaa ctgtaataat ctgctcacca tcaattaaat ttgattcaat
     7561 atagttatca tttaagtata tattatattt cttaatacct aaagataaac caattttctt
     7621 taactgtgtc ttaattgaag ctgaatcttt ctctgtattt tcttctgata gtttaactaa
     7681 tacatctaca gtatcaggtc ttgactcgtc aaaagtatta cttttatttt gtaaaggtgt
     7741 agaagctata ttcttaaaat aatcatttga aacgactgta tagtattgct tggcattatc
     7801 ttgataataa ttttctagaa atccaacaat aataaaatca cattgaactt ctttattcgt
     7861 aatatcatca taataattta atttaatgct atctccaata tctttatcta aaccaaattc
     7921 ctgtaaatat ttttctgaaa tagcaatttc atttttattt gtaggtagtt tgcctttcaa
     7981 tggttgaaaa ttcataagtt taattgctgt atcatctgca taaacaatcg acataataca
     8041 atctgtcttt ttattacttc caattaattt gtaagttcct acgccactaa agtttttatc
     8101 tttatatagt atattttttg ctttttcatc tacagattta tatatagcat gatatggaga
     8161 tgcattaact aaatctacag aggcattata tgaaactaag gtcactgtta ataataaaca
     8221 tgtagaaatc attataatga tacctgaaag atatgtttta agtttattaa tttttaaatc
     8281 atttaaagct atttttctta taaatgtgcg attattattt ctaaacatat actacacctc
     8341 ctagttaaca atttttccat cttcaatacg aataattcta tcagttagtt gtgcaatttc
     8401 attattgtgt gtaatcatta caattgtttg gttgaatttt ttacttgtca ctttaagtaa
     8461 actcagtaca tctgaactag ttctactatc aaggttacct gttggttcat cagctagtat
     8521 aatagctggt tttgttacaa gtgctctagc tatagcaaca cgttgctgtt gaccaccaga
     8581 aagattactt ggcatatcat ataatttttc ttcaagagaa agcatattta taatttcatt
     8641 tataaaatcc ttatctatgg tctctccatc caatcctatt ggtaagataa tattttcata
     8701 tacattaagt aaaggaacaa gattataatt ttgaaatata aaacctatat ttctgcgtct
     8761 aaagattgta agctgttcat cagtcatagt agataattcc ttacccttta taataacctt
     8821 tccagatgta ggattatcta atcctcccat catatttaat aatgtggatt tgccactacc
     8881 agatgtacct acaatagaaa taaattcgcc tttttcaata gatagagtta caccatcaag
     8941 agcttttgta atgtttgaac tttgtccata atatttcttt aaattaatta cttgtaaaat
     9001 actcataata atacctcctt aacatcttgt aaatacagta taaaaccaaa atgttgcaga
     9061 atcattacaa atgaaatatt taaaaattta tttattaggt aagaaaatag aaaaagtcga
     9121 cccttttcca atatctgatg taactttaat atagccacct tgaagagata taatttttct
     9181 tgaaagatag agacctattc caataccttc tatgtcatgg acatcttctt cacgataaaa
     9241 tcttttaaag atttctgctt ggcgactttc tgatattcct tttccattat caataatatc
     9301 tatttttgta tacataaccc aacattctgt agctacacgt atcttaccat tagaacttgt
     9361 gtattttaca gcattatcta atatattaaa aatagcttca gaagtccatt tcttgtcatg
     9421 aattatatat aaatttgggt cacaatcaac tgtaacttca atattctttt tttcagcatc
     9481 aaacaaaatt ccactaagtg ctgtagcaat tgtttcataa attgaatttt tctttttaga
     9541 tagtgtgatt atacctgtct ctagtctgga ggtttttacc atagattgca ttaaaaaatc
     9601 taatttatct aattggtttt ccatatccat taaaaaatcc ttttgaattt ctgttttaaa
     9661 tgtttgattt agtagagtag tattaatcat ttttaaattt gcaataggag ttttaatttg
     9721 atgagatata tcagaaacta gttcttgtaa atcagctttc tctatagcaa tactattgcg
     9781 attttcttga ataacttcat acattctaat taatctgtga ttgatacgtg ataaaatagt
     9841 ttcttcttct aattcaattt caatattatc ttcactattc atcatactat ctaaagtgtg
     9901 acatatttta gaacaaaata taactatttt tttatgaagt aaaaatattg ttaatgctat
     9961 aaatccacaa agtactattg tatatattaa taatagagca attagtttta aatcatcagt
    10021 tattagataa attgagatac taaataaaat agctgtaaaa attataccaa cagcaattat
    10081 taaataaata gaactaaaag aaaatttttt aaaattcatt tctttcacct aaccacatat
    10141 atcccatacc atataccgtt ttgatatact taagattgtc tttttcaatt ttgcttctaa
    10201 tcctacttat aacagatgtt aaagtatgtt catctacaaa gttagcatct atatcccaga
    10261 gtttttctaa aagtactttc cttgttaaaa tacttttagg attttcagta agcagtttta
    10321 aagtacgata ttctaatggt gtaaatataa tttgatttcc atttatattg gcagataact
    10381 cagaaaagtt aatttcaaga tatccatccc tatagtaatc ttgttttgtt tttactgtaa
    10441 gatgatttaa aaaagcttta actttctgtt taaatatatt gatattaaat ggttttgtta
    10501 tataatccaa agctccaagt tcatagcctt taatcatatc acattccata tcattagcag
    10561 ttaaaaaaat tattggtata ttgtaatttt cctttatttc attacataat tcaaacccac
    10621 ttttatctgg taaattaata tccaaaatta ttaaatcaaa ttcacattca aaaatagact
    10681 tcagtgcaga tttagctgta aattttgaag taatta
//
LOCUS       DAELJO010000135        10622 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELJO010000135 DAELJO010000000
VERSION     DAELJO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.135"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..222
                     /locus_tag="KPB88_002743"
     CDS             <1..222
                     /locus_tag="KPB88_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0055010.1"
                     /translation="YKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIGFGPSKENLA
                     HTIDEYIEQEQLFIGTEGYYGILKSVYGK"
     gene            355..1725
                     /gene="hydA"
                     /locus_tag="KPB88_002744"
     CDS             355..1725
                     /gene="hydA"
                     /locus_tag="KPB88_002744"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0055011.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLETKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1743..3149
                     /locus_tag="KPB88_002745"
     CDS             1743..3149
                     /locus_tag="KPB88_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0055012.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            3221..3667
                     /locus_tag="KPB88_002746"
     CDS             3221..3667
                     /locus_tag="KPB88_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0055013.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3706..4500
                     /locus_tag="KPB88_002747"
     CDS             3706..4500
                     /locus_tag="KPB88_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0055014.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMSLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            4490..6781
                     /locus_tag="KPB88_002748"
     CDS             4490..6781
                     /locus_tag="KPB88_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0055015.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGISLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKNLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYDPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6782..8095
                     /locus_tag="KPB88_002749"
     CDS             6782..8095
                     /locus_tag="KPB88_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0055016.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIEGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8131..9216
                     /locus_tag="KPB88_002750"
     CDS             8131..9216
                     /locus_tag="KPB88_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0055017.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9446..10584
                     /locus_tag="KPB88_002751"
                     /pseudo
     CDS             9446..10584
                     /locus_tag="KPB88_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4284 a         1112 c         2171 g         3055 t
ORIGIN      
        1 tataagggat taaaagaagc tggaatagat tcagaaataa ctcaatactc attctgtacg
       61 aatggaagtc actatgcagg tgaagctgga ataaaaacta taggatttgg tccttctaag
      121 gaaaatcttg ctcacacaat agatgagtat atagagcaag aacagttatt tataggaaca
      181 gaaggttact atggaatatt aaaatctgta tacggaaaat aatatttagt tcacaataaa
      241 ttatttaaat atagtctaga ggtgctgaat atagcacctt tagaaaatac aattttaaat
      301 aaagttttac aatacttaga aaatacttta aagcttaaaa gttaggaggt atatatggga
      361 acaatactaa gaggtggaac tataattacc tctgataaga cttatataag tgatttaagg
      421 atagaagatg aagagatagt tgaaatagga aataacttag aaataaatgg tgataaggtt
      481 attgatgcta caggtaaaat tgtaattcct ggtggaatag acacacatac tcattttgat
      541 atgaatgcag gttctattac aactgcagat aattttgaaa caggaacaag agcagctata
      601 gctggaggaa ctactacaat actagatttt gcagaagcca atgaggggga aaacctactg
      661 caaggtgtag aagcatatca taaaaaagca agtggaaatt gttattgtga ttatggtttt
      721 catatgacaa ttacatgttt aaataaagat acatttaatc atatggaaaa acttatttcg
      781 gatgggatag tatcttttaa gatgtatatg gcatatgatg gcatgaaagt agatgatgga
      841 acaatataca gggttttaaa gaaagcaagg gatttaggtt gtatagttgg atttcattgt
      901 gaaaatgggg atttactaga tgtattgata gaagaaaata tacaaaatgg taatttagag
      961 acaaaatatc atccacttac aagacctaac atagttgaga aagagtctgt ttcaagactt
     1021 gctgatataa ctaaattatc taattcaaaa tcctatgtag tccacttaag ttgtagggag
     1081 tcacttgaaa ctgtaaaaat ggctagagaa aaaaacatag atatgatagt agaaacatgc
     1141 ccacagtatc tattattaga agacaattta tataataagg ataaatttga gggtgctaag
     1201 tatgtaatgt ctcctccttt aagaaaaaaa gaagacataa attacctttg ggaaggattg
     1261 tctagtggag atattcaaac agttggcaca gaccactgtt catttaattt taaggggcaa
     1321 aaagacttgg gaattgatga ttttagtaag ataccaaatg gagcacctgg tgttgaacat
     1381 aggttagcac ttttatatac atatggggtc ttagaaaata gaataagtgt aaataagttt
     1441 gtggaagtta cttctacgaa tgctgcaaaa atatttggaa tgtatcctaa aaaaggtgag
     1501 atagctgtag gaagtgatgc agatatagtc attttaaata tcaataaaga agaaacaatt
     1561 agttataaaa ctcaaaaaca aaatgtagat tatactccat atgaaggatt taaggttaaa
     1621 tgtaaagttg aagatgtatt tttgagagga aatcatgtag tacaaagttg taatgttaaa
     1681 gaacatccaa caggtcaata tattaaaaga aaaattacta aataatgcgg ggggaaataa
     1741 gaatgatgga tatacttatt aagaatgcca taatagtaac tgtgaataaa gagagagaag
     1801 ttatttttga tggggcatta gtagtaaaag ataacaagat tgctgatatt ggaaattcta
     1861 aagagataga aagtaaatat actgatgtta aaaaaattat agatgctaaa ggtaaagttt
     1921 tattcccagg ttttataaat acacataatc atttattcca aactttatta aaaggattgg
     1981 gagatgatat ggtacttaaa gactggttag aaactatgac atttccagca gctaactatt
     2041 tagagccaaa agatacttat gatgctgcaa tgcttggatg tattgaagga cttagaagtg
     2101 gtataacaac aatggttgac tacatgtatc ctcatagtaa gccaggtctt tgtgatggaa
     2161 taatagatgc ttataaagaa ttaggaataa gaggaatact tggaagagga tgtatgaata
     2221 caggagctca atttggagtt catcctggaa taatgcaaga tgtagaaacg gttgaaaaag
     2281 atgtaagaag attgtttgaa aaacaccata atacagaaaa tggaagaatc aaaataggag
     2341 tagctcctgc agcaatatgg tcaaattctc aagaaatgct tgaaatgcta tggagagtag
     2401 taaaagaata tgatgatgct ttatttacag tacatatatc agagactcct tttgatagag
     2461 aagcagcaaa agaattgcat gggcaatatg acatagatgt tttagaaaaa ttaggaatac
     2521 ttggaccaaa tgtgcttatg gtacattgtg tgtatctaac tgaaaaagat atggaactta
     2581 ctaaaaaata cgatatgaaa gtgtctcata atacagcaag taatatgtac ttatcttcag
     2641 gtgttgcacc agtgccagaa atgcttaaaa aaggaataac agttagttta ggagtagatg
     2701 gagccgctag taataactct caagatatgc ttgaacttat gaaattaaca gcacttcaac
     2761 ataaagttaa taagtgtgac ccattagcaa tgtcagcaga aaaagttctt gagttggcaa
     2821 ctatagatgg agcaagagct attggaatgg aagatgagat aggttcattg gagataggta
     2881 aaaaagcaga cttactaata tttaatccaa tgctttcacc aaaagcgata cctatgcata
     2941 atccagtatc tacattagta tattcttcta gtatgaaaaa tatagagagt gttatagtag
     3001 atggaaatat aataatggaa gattcaaaaa tattgacagc taatgaagag aaagcattaa
     3061 aagatgctca agatacagct gagagacttt gtataagagg aactataaaa aatagaatgg
     3121 aaggtcataa atggaatagc ctttattaat aaatgtaatt tattcataaa aaagtatagt
     3181 aaaactttag cttaaaaata tgagttttga ggtgatttat ttgattatca aatgtaccat
     3241 aaataataaa gaaaaagaat taagtataag accagatgat tttttagcag atacattaag
     3301 agaaaatgga tatgtaagtg ttaaaaaagg gtgtgataca ggctcatgtg ggttatgtac
     3361 agtgatggta aatgacaagg ccgtgctttc atgtaattat cttactgcta gagtagaagg
     3421 tcagcatatt acaacacttg agggtgtctt ggatgaagca aagaagtttg gagattttct
     3481 tgcagacgaa ggggctgacc agtgtggatt ttgtgctcct ggatttataa tgatggtttt
     3541 ggcaatgaaa agagagcttc aaaatccaac agaagaagaa gttatagatt atgtaaatgg
     3601 taacctttgt agatgtacag gatataaagg tcaattaaga gcaatacata aatatttaaa
     3661 cacttagact tttaagtgaa aattaagttg atatggggag agagtatgat aactataaag
     3721 gaatatgtag ttccaaaaag tttagatgaa gcttatgaac tgctaatatc aagaaaaaac
     3781 aacataatac ttggaggatg tggattttta aaactaggta gtaaaaatat tggctcagca
     3841 atagatttaa aagacttagc tttagattat ataaatgaaa ctgatgatag catattaatt
     3901 ggagcagaca caagtttaag aactttagaa ttaaataagg ttattaaaaa ttactgtaat
     3961 ggggtaattt caaacgctgt ttctaatata gtaggagttc aatttagaag tggagctaga
     4021 gtaggagcta gcgtatttgc taagtatgga ttttcagatt taataccttc attactagtg
     4081 gtagatgcaa aggttaagtt atataaaaaa ggggttatga gcttagctga ttttttagaa
     4141 aatgagcttg aaaaagatat attaatagaa gttatattac ctaaaaaaga tgctataggt
     4201 gtatttgatt ctataagaaa atgtacaggt gattttgcag tattaaatgg agcaatgtta
     4261 aaagaaggaa gtatttataa aattgcgata ggcgctagac caagaagagc aaaaattgcc
     4321 tataaagcaa gtgaaatatt aagtttagaa aatgatatag aaaaagctgg tgaagtatca
     4381 agtgaagaac tttcatttgg aagtaatata agaggaagta aagaatacag aaaagatatg
     4441 gcaaaagcct tagtagtgag aatgtataat agtattggag gtgagtgtga tggtaaataa
     4501 agaggtaaga aaaatagata gtgaagccat tgttactgga aaaccaatat atacagaaga
     4561 tttaattttt cataaagatg ttttaacaat taagcttctt agaagtccac atgcttttgc
     4621 gaagataaaa aacatagata taaaaaatgc attaaaggta aaaggtgtag taaacatatt
     4681 cacttacaaa gatgttccaa attatagata ttcaatggtt ggagagtcat atccagaagc
     4741 atcacctcat gaccaattaa tacttgaaga cgtagttaga tatgtaggag atgaagttgc
     4801 tataatagcc gctgaagatg aaagggctgc tattaaagct atgaaactta taaaagttga
     4861 atatgaagta atgactccta tattggatgc aagagaatcg gaaggaaata aaattttaat
     4921 ccatgaagaa gatgatttgt tttgtccatt tgattttgga ttggatgcta caaaaaatat
     4981 tgtatcgaga gaaaaaatgg gtaaagggga tgtagaaaaa gagtttaaag aaagtgatgt
     5041 aatagtagaa cattcttata tgactcaatc acaagcccat tgtatgatgg aaactcatag
     5101 agcttatacg tatatagata cgaatggcag attagtagta acttctgcaa accaatcagc
     5161 ttatcatatg agaagacagg tttctaaagc actgggaata tctctttcaa aagttagagt
     5221 aataaaacca agaataggtg gaggttttgg tggaaaaaac atagctgtaa cagagatata
     5281 tgcatcattt gtaacttgga tgacaaaaag accagcaaaa ctaatttata ctagagaaga
     5341 aacttttgca atgactaata ctagacatca gatgtttttt gatataaaaa ttggggcaga
     5401 taaagaagga aacataaaag ctatagatat gaaagcattg aacaatacag gcgcttatgg
     5461 tgataatgga ccttcagttt gctctgaatc aggtcataat gtacttccaa catataataa
     5521 tgtacctgca ataaaatttg atgggagaac tgtatatact aatttagttc caggaggagc
     5581 attaagagga tatggagcaa cacaaggtac atttgcactt gattgtgcaa ttgatgaatt
     5641 agcgcataaa cttaatatgg acccaacaga aataaagtta aagaatatta taaaaaaaca
     5701 aactgaaggc ggaataatga attttccaat aagaagttgt gcacttgatg aatgtataat
     5761 tagaggtaaa aaccttattg gatgggatga aaagtaccct gttagggata ttggcaacaa
     5821 taaggtgagg gctgttggaa tgtctgtggg aactcatggt tctggtatag ccaacataga
     5881 catggcaata gtaaatatga ggatggatga agatggaact tataagttgt tttctgggtc
     5941 ttcagactta ggaactggtt ctgacacaat acttgcacaa atagctgcaa aagcattgaa
     6001 tacatctatt gatagaattt ccgtttatac aggagataca gatatgtgtc catatgatac
     6061 tggtgcatac gcttcttgta ctacttatgt aactggaaat gctacaataa aatctgctga
     6121 aagtttaaaa caaaagatat taaaagtagc acagaagaaa ctcgatttac caatagatga
     6181 tttaatacta tatgaagaca gagtttgtta tagtgaagat gtagagaagt ttgttttgtt
     6241 aagtgaatta ggaaaagaat ctgttggtgg tggaaatcaa gaagttttat attcatctga
     6301 atcttatggt atacaagaga gtgctaaacc attcttagca ggatttgctg aaatagagtt
     6361 agataaaaca acaggggagt ttaaagtaat aaattatgct tgttctacag attgtggtac
     6421 agttatgaat cctgcacttg ctagaataca agttgaaggt ggtgtaactc aaggtatagg
     6481 gcttgcaatg tatgaagacc ctagacatgg ttctgatgga aagcttcaaa cttctaatct
     6541 attacaatac aaagttccaa caaaaaaaga tataggaaac ataattgttg attttgtaga
     6601 taattatgat ccaacaggtc catatggagc taaatcttta ggagaaatag ttattcatac
     6661 acctgcacca gcaatagcaa atgcaatata caatgccaca ggagttcgta taagagaact
     6721 tcctataact tctgagaaag tttatatggc catgaaaaaa ttaaatcagg gaggtaaata
     6781 aatgattgct attaaaagta aatacttaat atcttctgct gatgaaatat atacaaattc
     6841 tgctgttgta gtagaaaatg acattataaa agatatattg ccaaatgaag aagttgaaat
     6901 aaaatataaa aatattgaag aaataataga taaatcagat gctataataa tgccaggatt
     6961 cgtcaatggt catatgcatc aatatggagt tctttctagg ggaataccgg caaatgtaca
     7021 ttttactgac tttgaaggtt tcttaaatga ctattggtgg ccatttatag aaaatagaat
     7081 tggattgaaa gaagtgaagg caacaactaa agcatcagca atagaactta tagaatctgg
     7141 tgtggtagct ttttgtgata ccctagaagc tccaaataca gaagaaggaa ctttaattga
     7201 gcaaggaaaa atactagaag aaataggcat gaaagcgatt ttatccttag aaagttgtga
     7261 aagaataagc tttgaaaatg gacttagatg tttagatgaa aattctaatt taataaagtg
     7321 gagtagagaa aatagtaaat taataaatgg aataatgtgt acacatacaa gcttcacatg
     7381 ttctgataga tttattaaga aagctaaaga agatgcaaaa aaattaaatg caccaatgca
     7441 atttcattta tgtgagagta tatatgagcc aaactatgct gaaaaacatt ttggcaaaaa
     7501 agcagttgac tactataatg atttagatat attggatgaa actgtattag catctcagtg
     7561 tgtaaaagta aatgatgaag aaatagatat attaaaagaa aaaggtgtaa aagtggttca
     7621 tatgcctctt agcaactgtg aagtaggtgg aggtttttct ccagtaccaa aaatgattaa
     7681 aaaaggtata aaagtggcac tgggtactga cggatatata aatgattttt ttgaagttat
     7741 gagaggtgca tttttaatgc ataaatcagt tgaagaagat gcatcagtaa tgccagcaaa
     7801 tttagtattt agaatggcaa cagagcatgg tgcttatgta ttaggacttc aaaattctgg
     7861 taaaatagca gttggtaata aggctgatat aatagttatg gaagatgaat ttaaaacccc
     7921 tgtgactctt gataatatat ttgaccagat agtagttcag ggtaaaaaag aatttatatc
     7981 aaatgtatac atagagggta gacatatttt aaaagaaaaa cagttagttg atttagataa
     8041 aaaagctatt gttaaagaaa tgaaagaagt tgcttgtgaa ttttggaaat tttaacgaat
     8101 gtaaatatat atgaaaaggg gaagcagata atgtataatt ttttaggtaa agaattaaaa
     8161 tcaccactta taataggttc aggaccatta acatatagtg cagctggatg caagatatta
     8221 agtgatgcag gagcaggggc agttgtgact aagactataa gaaaagaaag agctataaat
     8281 ccagcaccac atatggttag aaatacagct aatgctcttt taaataatga aaaatggaca
     8341 gattttgagc cagagcaatg gatagatttt gaaataccac aaatgaaaag agatgggact
     8401 gtctgtatag cttcagtagg tcatacaatt gaagaaagta gcgaattagt agagaaagtt
     8461 gcaaatgcag gagctgactt tattgaatta gtatcttatg actatagaga tttgatacca
     8521 atgcttaaag atgcaaagga aagagtaaat atacctgtaa tagttaaact tcctcctatg
     8581 atagatgaga taggcgattt tgctaaaaaa ttagaagaag caggagcaga tgcaataact
     8641 gcttgtgact ctgtaggacc agcatttaga atagacatag aaacagggca acctttatta
     8701 ggtggaaatg gaataggata tttaagtgga gaaactatca agccaataac tttacagaga
     8761 atatatgaaa taaaaaaaca agttaatata ccaataattg gcttaggtgg atgtgtaagt
     8821 ggagatgatg ccttagaaat gattatggca ggtgctgact ttgttggtat atgttcagta
     8881 gtaattttaa aaggcgcaca agtaatatct aaaatacatg atgatttaaa atctaatctt
     8941 aatagattag gatataatac tatagaaaat gcttgtggta tagtacataa tcatatggga
     9001 gatgttaatg aaagacttag ctttgagttt acttatgatg aagaaaaatg tactaaatgt
     9061 aagatgtgcg agagagtttg tgcatatcaa gcgagaaaat tgaatgataa gatggaattg
     9121 aatgaagaag agtgtagata ttgtggactt tgtatatcag tttgtaaacc aaaagcactt
     9181 ggaagaaaaa tttcatgttc agagctgaat gtatagatta ttttataaaa aacaaagttt
     9241 taaataaaaa taagaaaggc tatctcaaag agataagcct tttttgttgt tgaccttatt
     9301 tttgtttatt tgtagttatt ttagatatta attagttttg aagtaataaa gtgaatcaat
     9361 tttgtttaat taaaatttta ataattctga tatatatgtt aacattgtat agcatttgtg
     9421 taaaatgtta taataattaa tagggatggt gatgcaagtg caagagaaga ttttagagtt
     9481 attatatact ataaatgaag atgtagttag cataaaaaat gatatgtcgg aagtaaaaca
     9541 agaaataaat gtgttaaaag aagatatgtc ggaagtaaaa caagaaatga ctgtaatgaa
     9601 agaagatatg tcggaagtaa aacaggaaat aaatgtatta aaaggagata tgtcggaagt
     9661 aaaacaagaa atgactgtaa tgaaagaaga tatatccgaa gtaagacaag agataaatat
     9721 aatgaaagag gatatatccg aagtaagaca agaaatgact gtaatgaaag aagatatgtc
     9781 ggaagtaaaa caggagataa atgtattaaa agaagatatg tcggaagtaa aacaggaaat
     9841 gactgtaatg aaagaagata tgtccgaggt aagacaagag ataaatataa tgaaagagga
     9901 tatgtccgag gtaagacaag agataaatac aatgaaagag gatatgtcgg aagtaagaca
     9961 agaaatgact gtaatgaaag aagatatgtc ggaagtaaaa caggagataa atgtattaaa
    10021 agaagatatg tcggaagtaa gacaagagat aaatataatg aaagaagata tgtcggaagt
    10081 aagacaagaa atgactgtaa tgaaagaaga tacatcagag gtaaaacaag agataaatgt
    10141 aatgaaaaaa gacatgtcag aagtaagaca agaaataact gtaatgaaag aagatgtttc
    10201 cgaagttaaa tcagaagtaa actttatgca aaataaaata aataacatta atgaagatat
    10261 gaatggaatt aaggacgagg tatccatagc aaatgaaaaa ttatatggaa ttgaaattaa
    10321 gattgatagt ctagaatgtg aagataaatc aatgaaagag atacagatag aacagaataa
    10381 tattttagga agtttattac ataattctga aataaataaa gctactcatg ataatataga
    10441 acataacata gcatagcata tataaaaggt gacactaatt ctataaaaga ggatatagct
    10501 gaaataagaa gagacttaaa tttagtagag ttagccacta gtaaaaattg gagtgatatt
    10561 gtcaaactaa aatcagtaaa gtagtatttt aattcactaa tttatcatcc acaaactaag
    10621 aa
//
LOCUS       DAELJO010000136        10579 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELJO010000136 DAELJO010000000
VERSION     DAELJO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.136"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(174..800)
                     /locus_tag="KPB88_002752"
     CDS             complement(174..800)
                     /locus_tag="KPB88_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0055018.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(876..1847)
                     /gene="nrdF"
                     /locus_tag="KPB88_002753"
     CDS             complement(876..1847)
                     /gene="nrdF"
                     /locus_tag="KPB88_002753"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG0055019.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(1860..3959)
                     /gene="nrdE"
                     /locus_tag="KPB88_002754"
     CDS             complement(1860..3959)
                     /gene="nrdE"
                     /locus_tag="KPB88_002754"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG0055020.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4437..5162
                     /locus_tag="KPB88_002755"
     CDS             4437..5162
                     /locus_tag="KPB88_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0055021.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(5290..6486)
                     /locus_tag="KPB88_002756"
     CDS             complement(5290..6486)
                     /locus_tag="KPB88_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055022.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(6489..7514)
                     /locus_tag="KPB88_002757"
     CDS             complement(6489..7514)
                     /locus_tag="KPB88_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0055023.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(7586..8716)
                     /locus_tag="KPB88_002758"
     CDS             complement(7586..8716)
                     /locus_tag="KPB88_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055024.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(9310..9705)
                     /gene="tnpA"
                     /locus_tag="KPB88_002759"
     CDS             complement(9310..9705)
                     /gene="tnpA"
                     /locus_tag="KPB88_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0055025.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(9870..>10579)
                     /locus_tag="KPB88_002760"
     CDS             complement(9870..>10579)
                     /locus_tag="KPB88_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055026.1"
                     /translation="VISRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIEN
                     NSNEITKKIDTQLDKVENFVEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDF
                     INKNIKLELGEINKTVYSDSKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVL
                     TIEDNGVGIVERDINKVFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKV
                     NVGTVIKVSFPIDYNLF"
BASE COUNT         3419 a         1858 c         1111 g         4191 t
ORIGIN      
        1 aataatactt atataattag tattattatt taatagcaac agttattcac actaataaaa
       61 gtagaaagtt tttaaaatat tatgaaacat caaatttaat tttaaaattg catcaaaaaa
      121 tgaagtctca acagacttca ctttttataa ctcttataaa ttattcaaaa tatttatact
      181 tccctttcta cttcaaaacg taaaaccgtc ttcaatgatt caggcgatac ttttctagca
      241 tcacttatat aaatttcacg atgtgtgtaa gattttctta taagtttatt ttttttgcaa
      301 aattcgttca taatgtcaaa gctttctggt tcattatcat atgaacctat atgaagcatt
      361 tgaacacata gcccttcatt aatagaccca aatctaactt tttcaagaag attatgaggt
      421 tttttcttct ttactatatc agtagcttta tccaacaggt cttgtgtaac aaaatctggc
      481 tgacgtatca ttatagtgta aattaaatct tctttaataa gtacatctaa gcctttactt
      541 ttttctccct tatcccatat accttctaaa ggaaaaaccg tatagtcaaa atacccttct
      601 ggtacaattc cttttttagg catggttttt attgcatatg ccaaagaata aagtactcct
      661 attgcttcag taaattcttt agaatttggg tctccactcc cttttaacat gataaatttc
      721 tgttctggta tttcaactat tacaggattc tttttaggta aatataactc tttatctctt
      781 tttctccatt catatttcat aaaaatacct ccatatatat tttatacatt gatattatca
      841 cgctaaaagt gacaacagtg tgtcataatt ataaattata ttataaaatc ttcatcctct
      901 aattcctcat acactccttt ttggtatcca ttacccttag ttgaaaaaaa atcatgtgtt
      961 ttggtttcag tgctaagacc atttaataca attggattta tagcatctac agtatatagc
     1021 ttctcaaacc ctagattttc taaagctcta ttcgcattgt attttaaaaa gttcacaacc
     1081 tctttttcaa gcccactttc tctgtatata acatttgtat actctatctc attctccata
     1141 aggctataaa gtatagaata catcttctct tctaacatct ccttatccat tttatcaaat
     1201 gaatcgtata tttcttgtgc tagcaatcct atatacttac catgtaaaga ctcatctcta
     1261 agaataagcg atattatttc tccactagct accatctttc cttgaccaga tagatacaat
     1321 ggataaaaga atcctgaata aaacaagaaa ctctctaaaa atacacttgt aaccattgat
     1381 aggtataatc cctcttgatt tgtagtattt tcgtattgag ctaaaactaa atcagccttt
     1441 ctttgtaatg ttggttctgt ttctatccat tcaaataatt catctatttc taaattagaa
     1501 agtagagtca taaatataga agaataactt tttgcatgta tttcttccat agtccccata
     1561 aaagacaaga cagcttttct ttgtaggttt tctgttaaac tcatcataga tggtattcca
     1621 ttattcccct gttttgtatc cagtaatgtt aaaccaccca atactttttt atataattcc
     1681 ttttctttat tacttagttc attccatacc tttatgtctt tagaaacaga tatttcttca
     1741 ggaagccaaa attgttttac attttgttcc caaaaagctt gtgtaaatcc atcttcttct
     1801 ctattccagt taactgcatt atgtatttta tgtaaattaa atgtcatata tttctctccc
     1861 tatactgaac acactagaca ctcatgagta tctcttgtca tctttgttct tgtgtaatat
     1921 aaacttttca aacctttttt ataggcatat atgtaatgtc tagctatatc tctagttgtc
     1981 ttttcatctg taacaaacag tgttgttgat ataccttggt caacatggtt ttgaactgtt
     2041 gccactaaat ctatgacctt tctcatatcc atcctgtatg ctgattgata atacaacata
     2101 ttatcatttg ttaaaaatgg cattggatat attgtagtag agtctccata tgttcttact
     2161 tctactggct cagtaattgg cattatactt gatgtagcat tttgaacata actaatgctt
     2221 tgagttggtg ctactgccat taaatatgca ttatatatcc ctttttcttt tacctcatcc
     2281 aataattttg tccagtcttc ttttgttggt atataaatcc cttcaaataa tgctcttacc
     2341 ttttctgact taggtagata agattgttca tagtattttg ataaaacctt gctatttctt
     2401 cccttaacat attctgattt atcaaatcct tcaaaagttt gatttctttc tatagctatt
     2461 ttcatagaag ctttaattga atagtatcta accattgcaa aaaatacatt agaaaaatct
     2521 atagcatcat ctgaagtata taaaatattt tctcttacta agtatccatg taaattcata
     2581 gctcctaatc ctatagaatg agacttgctg ttactattgc ttaccgttgg cactggtttt
     2641 atatctgtgc tatctgctac aaatgacagt gctcttattg ctgtttcaac tgtactttct
     2701 atagttttat tatccattac attggctatg tttaaagaac ctaggttaca acttatatct
     2761 tgtccccatt cattttttcc tccatacccc tctatttctg aaggtgtttg atattgaaat
     2821 atctcacaac ataagttaga cattttaata attccaacat cttttaatgt atgttctcta
     2881 ttagctgtat ctatgtacac tacatatgga tatccacttt cttgttgcat ctgtgctatt
     2941 tttgtcaaca tctgcctagg atttatttct ttttttctta tatcactatc attaaccaat
     3001 ttatcatacc actcatccat ttctatctca tcaagagaaa ctccatattt cttatacaca
     3061 gtatgaggat aaaatgcata tgctacttca tttttttctg ccaactccat aaatttatct
     3121 ggtattatag ctcctaatga taatgtagca agccttattt tttcatctgc atttatcttt
     3181 ttagtatcca tcaacaattc aaaatcagga tggaatacag taaggtacac cgcaccagaa
     3241 ccttgtctag ctcccatttg attaaagtaa ctaaatgatt gttctaacat tttagctact
     3301 ccaacaacac ctccagctgc accttctata tctttgatag attcgccaga tgctctaagt
     3361 ttagataaat ttagtgcaac tcctccacca atctttgata agtgatttga tgaagatata
     3421 gcatacgata taccttctgt actatcttct acagatagta aaaaacatga taccatttct
     3481 ccagcccttt ttcttccagc atttaaaaaa gtaggtgtag ctggttgaaa ttcttgtttt
     3541 actattttat ctgccaagtt taaagctaaa tctttattgc catttcctag tgtcaaagct
     3601 acaattaaca ctttatcatt atatgtttct aatatttcct ttccatcatt gctctttaat
     3661 gcataatttt gataaaactt atttgcactc atatatgatt gaaattcaaa cttatgttta
     3721 tcaatcatat catatatatt tttaataaca tctatgctat actgatttat tacttctttt
     3781 gaataataac cttcatctat taaatacttc aatctttctt ctaggttatt aaattttctc
     3841 aactttggct gtataaattc ttctatgtaa ctacttaagg cttccttatc cttttccaat
     3901 tggtacttgc cttgttcatt tttaattatc acttgattgt taagctctat ccaattcatt
     3961 atttaccgtc cttttaacat aatcttgtgc cataatttca cttaatttgc ttattatttc
     4021 ttttggtact tatcagttga aatcccaaaa ttaatctata attatagaaa tgcaataagt
     4081 atttttcaaa aacaaaagca ctacatatag tcagtgcttt atctattata tactacatat
     4141 agtatataca caaatttttt tatatctaaa cttttattac tttataattg acattatttt
     4201 tatttaaaga aatttttatc aactcaaata taaaacaata caagcttatt atgtcctgtt
     4261 taaaaattta aattatctct aaatagctta aaaatatatt ctgatatatt gaaaaaagta
     4321 ctttcaagat gatattttat atattattta gaagtttttt atttattgtt atataattaa
     4381 atatatgact attttgctac tacatatata aaaataaatt tatgaaggtg attttaatga
     4441 aaactatttc taaacaagaa ttagaaaaaa cacttgagaa acattattta tggcttaaaa
     4501 acaataatgc tggagaaaag gcagatttaa aagaattaag attagaaaat atggacttaa
     4561 gaggatactg cttaagtaat attgactttt cgtggtctga ccttatcaat ttaaatttag
     4621 aaaaagcaga tttagaaaac tctattttta ataattctta tctttcggat tgttcattaa
     4681 aaaattctat attaaaaaat gcagacttaa gtggtgcaag ttttagattt tgtgacttat
     4741 ctaactcaga tattagagga gctaatttag aaaattccaa tttagaacat gctactttaa
     4801 atggagtaat atcagatgaa agtactcgtt tttttaaatt gtattgtcct gaaacaggag
     4861 catttatagg ctataaaaaa tgttttaatt ttagaatggt acaattatta atacccagcg
     4921 acgcaaaaag agtgtctgct acatcaaatg cttgtagatg tgataaggca aaagtactgt
     4981 caattactag tatagatggt aaagaatctt ttaaaagtgc tcgtgcatat gctgatgaaa
     5041 attttatata tagagtagga gaaacgattg tagctgaaga ttttaatgaa aatagatgga
     5101 aagaatcgac tacagggatt cacttttttt taactagaga ggaagctatt ggttatctat
     5161 aaaatgctaa agttttataa atatataaat acataaacaa aataacaaaa gagtatagat
     5221 tgtaatttca aaaaaattat aatctatact cttttttcta tagcacataa gtacatttta
     5281 tttcataaat tataataaat tatttatatc tgcactattg attatttgag aaactttatc
     5341 tatatgttct atcttactat ccaaactgtc ataatacttc ttactctcat ctctatttct
     5401 aaaagaataa acttttttat ccaattttaa agcctctttt ataatatcta tattcctctt
     5461 atttgtctct cctacactaa atcctgtatc tattataatc ttggaatcat ttaaatttct
     5521 aatagccaaa tcgaaactct catcacttat aggctcaaat ggattttctg taaacatctt
     5581 tatccccata gttctaccaa tttcataatc aatatcattt tcatgtatta ttccagaata
     5641 cagacctata ccttttttag taaaagctct ataaattggt gttgctttac ccccaccacc
     5701 aattataaaa acttcatttt tagatttatt actaatttct actgaaccta aaagactatt
     5761 aaagttttta tcatccaatt cataaagcga atttataata tcctcatcta ctacatcctc
     5821 aggctgacca taagctatga ctttattatt ttttataaga gcaactttat cacaactttt
     5881 taatgctatg tctatttcat gcaatgaaag tattactgat attgactttt ctttacttaa
     5941 ttttttcagt atgtttatga gttccaatct atgttttata tccaagtgag ttgtaggctc
     6001 atctaatata attatttctg gttcttgaac aagagccctt gcaaccaaca ccttttgttt
     6061 ttctccatca cttagttcat caaaatatct tttctttaaa tgtaaggcat ctacagactc
     6121 caatgcttca tctaccatta ttaaatcttt cttagataga ctcccaaaaa atcctgtata
     6181 tggatacctt cctatattga ctatatcttg tacagtcatt aaatcaccta ataatctatt
     6241 tgtaagtact aaagccattt tttttgataa tgcttttctt gatatacaat ttatgtttac
     6301 accattcaaa tatatttcac cttttattgg tgatataagt ttagataagg accttaatag
     6361 ggtagttttt ccagctccat tacttcccaa aagacatagt atttctccat tttttacttc
     6421 tacatttata ttacttataa caactttgtt attgtatcct acacttaaat tattagtttt
     6481 caacatgtct aagccctatc cttccttttc accaataaca ttactacaat tggagctcct
     6541 aataaagatg ttatcgtact tattggtaat tctactggtg ataaaattac tcttgatatt
     6601 aaatcacaaa gagctgtaac tatactgcct aaaaatatta ctactggtat caaaactcta
     6661 ttatctgatg ttttaaatat catttttgaa atttgtggca ctgcaagtcc aataaaccct
     6721 ataggtcctg caaatgctga tactattgct gctaatatac tagatataaa tactatcaaa
     6781 actctaaaaa atcttatatt tatgccaata ctttttgcat actcttctcc tagtaaaaaa
     6841 gcatttaaag gttttattat gaatacagtc atcaaaagac ctaatatacc aactattata
     6901 agtatttcta ccttttccca tgtaaatcca gaaaaacttc ccatagacca catagtaaat
     6961 tcttttaatt tatcactatt tgcaaatgat tgtaacacat ttgttatacc actacataca
     7021 tatccggtca taattcctat tattaaaaga gttgttatat tctttacctt atttgcaaat
     7081 aaaatgacta tagacattac aattaaagct cctataaaag ctgctacaaa tatactcatt
     7141 gaatttacac tctcaaatcc caatgtgtat cctcccaaaa tcatgagagc tactgataaa
     7201 gtagaacctg aagatactcc taatatatac ggttcaacta ttgggttttt aaaaaatatc
     7261 tgtaataaaa tacctgatac tgctaggcat gctccaccta ccatggttgc aagtactcta
     7321 gggattctta tattgtatat tattttataa tgttctgtat tatccatacc tcttaataca
     7381 acatctatta cttctttaaa ttcaattttt acagagccaa atgctacaca cactaaaaaa
     7441 gtaagtatca tacttataat taatactata aatattgttg tattccttat tctcatttta
     7501 ttgataaaat ccatttgtat actcctctta gcaaagctat attaactaaa atctataaaa
     7561 cctatctttt tgtgtcctct ctgtactatt tatcataatt tatataatgt ttgatttttt
     7621 cacctgtagt acctgggtgg aacatctcaa ctaaatctac tattaattcg tccgtcttat
     7681 ctactgattg ataataatct ggagcaaaca cccataggtt tccgtttttt acaacatcca
     7741 aatcagccaa tacaggtgca ctagatttta tagaatctaa atttggtgca taatctgaac
     7801 ttgatgagta tacaaatata tctgcatctt ttgcaacttc atgcaattgt tcaatactta
     7861 ctcgcccttc atttgaatct attgaagcac atgcatttat tccaccagcc atttttatca
     7921 tattatccac atatgaatct ttaggagcta tataagcact tccttcatat acagatgccc
     7981 atacaacttt aggtttagct ttgtttttta tttttttact tacttcttct accttttgta
     8041 cagttttatt tagttcacta gtagccatat cttctttatc ataaaatgca gctattaaac
     8101 ttgtccactc cattcttcca aatggatttt gctctttata ctcattgtct acaacataat
     8161 taagccctaa ttcttttaat ttgtcaataa attttgtatc agcctctgat aaaccactac
     8221 tcactatagt taagtcagga tttaagtcta ctattttttc ataatctggt gctaaatttg
     8281 aaccaacgta atgtattttt ccatctttca ttaaatcttt aatttcttta atagtccagt
     8341 actgctcttc tgttgttacg gcttttatac tatctagttc atctattgtt ctcagagaac
     8401 aagcctgtgt tgtagatgct attaatacat tgtccacagg cgttctaact atattagcat
     8461 ttttatattt ttcaggaatt ttaatcttct ttgggattaa aatcagttct cgattatctc
     8521 catcagttac ttttttaatt ccatcttcta aatcttcaac actaaatcca ctagcatatt
     8581 ttaattctaa tttcttacca gtactagctt gtttattctc tttgtcactt ttactacacc
     8641 ctataagacc tcctaccata aaaattacca tagcaattaa aaaaaactgc tttaaaacat
     8701 ttgatttaaa tttcatctct ttcactccaa tatcttattt tttatgagtg gctaaaaaac
     8761 tttaataaga tatacctttt aaagtatatc ttattaaagt tatattaaac catctttaac
     8821 aagtataact ttttaataaa attatagtga attttgattt cacatgcaat taactactta
     8881 tacacttaac tacctacgac aacgtttgaa gtggcatctt ctttgtcaaa atagctattg
     8941 ttattagtat ctccaagcta ataggatagt tcccaacact tacgattagc atctgagtca
     9001 ctacgttggc aaactaatag aaatataaca tttatttgat gatgctttag atacagtaca
     9061 actctatcaa attgcctatg ttgttttatc ctaatcattg attttaattt aagcagtttt
     9121 atatacccat ttctatatgc acaatatctt ttgagtaacg tgttgacaaa ctattttggt
     9181 tagtagtata gctatgatag ttaattttct ttgatagata agtccgttac atccaaaatt
     9241 cttaggaaaa tattcctttt attcactgtt tgtataaacc tgtacttata agctcttaac
     9301 atattatcac tattcttctt gattttctat gtattgttcc aaatcttcca atgttgcacc
     9361 accagttgtt actaaacaat aacttttact ccaaaagtat tctttccata agtatttgcg
     9421 gatacttgga aactcattct ttatccttct gcttgatgca gatttatagg catttaataa
     9481 cttggaaatt tctgtattag gctttgcttt gaataataag tgaacatggt caacataatg
     9541 attccattct atcaacacca ccccataatt tggtgcaata tctacaaata tatcttttag
     9601 tctatcagat atagaatcat ctattacttt tcttctatat tttatacaca taactaaatg
     9661 ataatgcaat aagaagactg aatgattatt actatctaat ttcattataa ttacttccct
     9721 tcagctttta ctactaaatt aatgatataa tacgttttta tgaaaatcaa gtaaaaaaaa
     9781 gaacctatca attatgctta catccttaac tttcttaaat aaatacaaaa aactgacaac
     9841 taaaaattgt cagtttttat aactatacac taaaataaat tgtaatctat agggaatgat
     9901 acctttatga ctgtaccaac atttactttt gattctaata ttaaacctaa acctaactta
     9961 ttacataact ttttacatag atataatcct attccagtac tttttccaaa tactcttcca
    10021 ttctctcctg taaacccttt ttcaaatacc ttattaatat ctctctctac tatacctact
    10081 ccattatctt ctatagttaa cactattgag ttttctaccc tttttgcact tatttttatt
    10141 aaaccatctt ttgactttat atattttata gaatttacca caatttgatt taatataaac
    10201 tcaacccatt tcgagtcact atatacagtc ttattaattt caccaagttc taattttata
    10261 tttttgttta taaagtctct atagtttctt ttgataactt catttacaac ttcttcaagg
    10321 tttagctttt ttattatata atctttattt acattatcaa tttttgaata atataatact
    10381 tgttccacaa aattttcaac tttatctagt tgagtatcta tctttttagt aatctcatta
    10441 ctattatttt ctataataag tttactagat gctataggcg tttttatttc atgaacccaa
    10501 gtttcaatat attctttata ttcttcttga ttatcattat aatatttaat attttcgtgc
    10561 atgtttctac ttattactt
//
LOCUS       DAELJO010000137        10503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAELJO010000137 DAELJO010000000
VERSION     DAELJO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.137"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..345
                     /locus_tag="KPB88_002761"
     CDS             <1..345
                     /locus_tag="KPB88_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055027.1"
                     /translation="YSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLG
                     LSMKSFSRLVRINKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRA
                     NMSDFYSEIAKF"
     gene            383..844
                     /locus_tag="KPB88_002762"
     CDS             383..844
                     /locus_tag="KPB88_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0055028.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            875..1123
                     /locus_tag="KPB88_002763"
     CDS             875..1123
                     /locus_tag="KPB88_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0055029.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGST
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            1576..1896
                     /locus_tag="KPB88_002764"
     CDS             1576..1896
                     /locus_tag="KPB88_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG0055030.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            2034..2678
                     /gene="trmB"
                     /locus_tag="KPB88_002765"
     CDS             2034..2678
                     /gene="trmB"
                     /locus_tag="KPB88_002765"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG0055031.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            2807..3553
                     /locus_tag="KPB88_002766"
     CDS             2807..3553
                     /locus_tag="KPB88_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055032.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(3682..3960)
                     /locus_tag="KPB88_002767"
     CDS             complement(3682..3960)
                     /locus_tag="KPB88_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055033.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            4235..4681
                     /locus_tag="KPB88_002768"
     CDS             4235..4681
                     /locus_tag="KPB88_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055034.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            4951..5403
                     /gene="accB"
                     /locus_tag="KPB88_002769"
     CDS             4951..5403
                     /gene="accB"
                     /locus_tag="KPB88_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG0055035.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            5419..6780
                     /gene="accC"
                     /locus_tag="KPB88_002770"
     CDS             5419..6780
                     /gene="accC"
                     /locus_tag="KPB88_002770"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG0055036.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            6795..7649
                     /locus_tag="KPB88_002771"
     CDS             6795..7649
                     /locus_tag="KPB88_002771"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG0055037.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVITGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            7670..8614
                     /locus_tag="KPB88_002772"
     CDS             7670..8614
                     /locus_tag="KPB88_002772"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG0055038.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            8803..9063
                     /locus_tag="KPB88_002773"
     CDS             8803..9063
                     /locus_tag="KPB88_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG0055039.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            9216..9587
                     /locus_tag="KPB88_002774"
     CDS             9216..9587
                     /locus_tag="KPB88_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0055040.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            9617..10462
                     /locus_tag="KPB88_002775"
     CDS             9617..10462
                     /locus_tag="KPB88_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055041.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFFHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         4088 a         1103 c         1737 g         3575 t
ORIGIN      
        1 tactcatatg agtttatttt ggcaattcag ttgattattc aaaactctgg aaatatatct
       61 tcacaagaaa tttctaaaaa agtattttat agttctaggc atttaaacag actctttaat
      121 cagtacctag ggttgagtat gaagtcattt tctcgtttgg tacgtattaa taaatctata
      181 aaacttctaa acaataataa aacttcattg atgtctatat gtaatgaatt aggattctat
      241 gatataccac attttattaa agattttaaa attgtctgtg gaataactcc tcaaaaatac
      301 cgagctaata tgtctgattt ttacagtgaa attgcaaaat tttaagttat aatacttata
      361 acaaagaaag gatagtgata agatgaaatt tggaggagta ttgctaattg tcaaagacat
      421 aaataaatca aaaaagtttt atgaagatgt tatggagcaa aaaatcacat tagatttagg
      481 agaacatgta tcatttgaaa atggatttgg attacaaagt aattataaag ctataattgg
      541 atatgattta ccaatatatc aacaatcaaa taactttcag ttgtattttg aggttaatga
      601 catcaaacaa tgggaaagta agataaatag aataggaaat attgaatttt tgcattatat
      661 aaaagaatac ccatggggtc aaagaacttt taggttttat gattttgata aaaatattat
      721 agaaatttca gaaagtatgg aaagcgtcat aaagagactt ttaaaacaag gtttagcttt
      781 agaagaaatt tcaaaacgca ctatgtatcc agttgaattt attacacaat ttcaacaatt
      841 ataaaatatt aaaatatata attttttctt tacattgcaa aaaacaaagg cttggggcat
      901 aatagaacca gaaaaacctg aatctcaaca gcaaatggct catatggatt tcgctgttaa
      961 tgatttagaa gaagcagttc aatatgcaat tcattgtgga tctacaatcg cagaggagca
     1021 atttactgat gactggagag ttatgattga cccagctgga cacccttttt gcttatgtca
     1081 gatgaaatca attatggaaa gtagccattt ttcattgtta tagaccaaaa tattgtttat
     1141 taattacaaa aaagcacaac agtatatacc gttgtgcttt tacttattat acatgaatac
     1201 actaaaaaat ataaatagta gagctttatc aataatgata atttttaaaa aatagtattt
     1261 ttttattgaa taatttctta gatattttaa agtatataaa ttttgcatat atgaattgga
     1321 cattatcaaa tgtgttataa tattaaaaca acaaataaaa ttattatatt ttttagaata
     1381 ataattttat ttgtttacta agtttatgat agggtattta tttaaggtaa taaaatatta
     1441 tataaaatat tttaaagcct taggtaatat taggtagaag ttttagtaaa atatgcaaaa
     1501 actaattgat tatgtacctt gtatgggtat ataatagata ttgttttaat aaaatatttg
     1561 aaagggaatg atttgatgaa aattacttta ccagaaacag ctattgatac tttaaaaagt
     1621 atcttaaaag acaatcaaga taaacctaat aatataagag tatactttgc aggtgttggt
     1681 tgtggaggtc cttcttttgg attagcttta gatgagaaaa aagaagatga cttaacttat
     1741 gaagttggag aattacaatt tgtaatgagc tctgatgaat atagtcagta tggagatata
     1801 ataatagaag atacaggatt tggatttaga gttattcctg aaaatatgaa agatcaaggt
     1861 ggaggaggat gctctggttg ttcagggtgc cactaagatt aagtttttag taagtttaga
     1921 actctcttta tagaaataaa tttctatttt aagagagttc tttttatttt ttggtgagat
     1981 aatgtataat aaaaatgtat tttactaatt ttttatatgt aaggagaatt attgtgagaa
     2041 gaagacgaaa aaaaggtgca gatgaaaagc ttttaagtta tacaaaatat gttttaagag
     2101 acgatataga caaattaaaa ggcaagtgga atttaaagtt tagaaatgat aatccaatac
     2161 atgttgaatt tgggactgga aaaggtaagt ttataacaac tttagctaag cagaaccctg
     2221 acataaatta tattgccatg gaacttaagg aagaagttct tttaaaagct gttgaaaaag
     2281 cagatgcttc taatttgaat aatatactat ttttatgggg agatgtaagc aatattttgg
     2341 attactttga agctaaagaa ttatcgagaa tttatataaa tttctgtgac ccatggccta
     2401 aaaatagatg gagtaaaaga agactcactc attctggatt tttagaaatg tacaataggg
     2461 ttttggaaga tgatggcgaa attcacttta aaacagataa tgaaaaatta ttcgagttta
     2521 gtttaaatga aattgctgca aataattggc tattaaagaa tatttctctt gatttaggaa
     2581 atagtgagta tgaaaataat gttacaacag agtatgaaga caaatttatg tcacaaggta
     2641 tgcgaatatt tagatgtgaa gctaaaaaac gtaactagta atatgtttat aagtttaata
     2701 gtagaaattt attttaattt atattaataa agtgtacgaa agtaggtata tataacaagg
     2761 aaacagaacc ctctcataga aattaaatta ttataataaa aaatttatgt gggagggtac
     2821 tatgaattta gctaatttaa taccatttat tatttctgca gcagctttaa gtccaataaa
     2881 ttcaaatgat aaggttttga gcgaaaatca acttaaagat atttttcctg atgcaaattt
     2941 aagagcagtt gtaaaaagat atataaatcc agatgagatg actataagca atataaaggc
     3001 tttagatgga gaattttatg caactggtga gagtatttca aatttaaaag gtatatcata
     3061 tcttgaaaat gtagacaatt ttattttttg gaataataat ataaaagagg ttcctaagga
     3121 agctttaagt ctaaaagata tggattcaat aaatcttgcc aataattatt tgattgatga
     3181 cgatgtagta aattctcttt ctcataatgg tgtggatgta aattgcgatt taaattttat
     3241 tgatacaaaa gataatcagt ataaactgga ctctaaatat cataatgttg atatagcaaa
     3301 aggtgaaagt attgatttga gaaaaataat tacgaagaaa attgatagtt attataaata
     3361 ctgggaagtt actgataacc ttccaaaaga tttaaatttt attgtatctg taagtgataa
     3421 gagtgtactt tcatgtgaag atatgataat taaaggtaat aaaacaggtc aagcagtggt
     3481 aaaagttata ctaagcgaca aaaatgattt aaatacatct caagaagttc taattactgt
     3541 caatgtaaaa taatctatca acttattcaa tcattctatt ttcaatttaa gtgtataatc
     3601 aaaataaagg ttgttagtaa cagaacgcta atattagtga tttgttacta gcaaccttta
     3661 aattttatag aaatagatta tctaaaaaat tccatttctt ctgttagtag cccatattcc
     3721 tattaaaatt atcagtgaat atattatata caaaactatt cttatatatt ggacatctaa
     3781 tccaaaaaat tcaatgttac tctgtgaggt atgcaacatc acaaggactg cagatatacc
     3841 aataagcaaa ggattgatta ctatttgctt atcagttctt aaaatcatta ttttacccat
     3901 ccaaattatt gataatataa aaaataaaat tttaagacca aattctaatg tatctaccaa
     3961 agcgaaaaca tcccttcatt tataataaat ttttcagctc aacaaaatat aaaaccctac
     4021 aaacaacttt tgaatccata ataagataac ttgattaagc actatgaaat aagtactata
     4081 aaacttcatc tacattaata tatactacac tatacttcaa atttcttaaa cataaattaa
     4141 tgaaattaac aaaattttat tcagaaacat ataaagatat attttactat ttaaatgcat
     4201 atcattatat atacagtttt aggaggtgag gtatatggaa tctaaatttt ttaattggta
     4261 cactcaatcc ttagggggaa cttttggcat tatatcatgt atatgcgcat atttaaatgg
     4321 atacatgttt gtttatacaa atagtgctag caaatatttt gaaagtatag gatttttggg
     4381 aataatttca agttatacac tacttccact ttgtatgctt actttgattt ttggagttat
     4441 atattcttat actccagaaa catccgacaa aagtattttt aatatacctt tttttacttt
     4501 aaataaaaga ttaatactta ttacagtagt ttttggtttt ataggtgcta gagtttactt
     4561 tataattcct gcaattctaa taacttttaa cctatatagc aatgttttga tagctaaatt
     4621 aaagtacatc ctatgtaaaa ataaagaaaa tgatactaat ttgaaagaaa gcgaagtcta
     4681 aattgcataa tattggtatt tgtcttaaaa attcccgagg tattaatttg tattctcttg
     4741 tattttgcaa aaaatattga attttattca ttttatactt ataatttata ttaataatgg
     4801 aaaattaaaa caaatataag ttactggttg ttaatttaaa attaaaagat atattgatgt
     4861 gttgattctc tagtcagcac tttttctttt ataaatttgt tttttgtgat ataatattag
     4921 caccaggtaa taatacaagg gggtaacaaa atgaacatca atgaaattaa ggaattatta
     4981 aaggccatag actcaactaa cttagaatat gtaaaacttg aaagtagcga tttaagatta
     5041 gaagtatcta aaaaagctca atctacaagt tcacctgttt taagtgtaca acaagaatca
     5101 gtagtggatc tttctttaga aaagccagtt gtaaacgata cacctgtcac atctaatgag
     5161 aatctatcag tagtagtagc tcctttgatg ggaacattct atgattctcc aagtccagat
     5221 gcagatagct ttgttaaagt tggcgatgta gttgaagaag gcgacacttt atgtatatta
     5281 gaagcaatga agcttatgaa tgagataaca agtgaaatta aaggtgaaat aattgaagta
     5341 ttagttagta atgaagaact agtagaatat aatcaacctt tatttaagat aaaaccattg
     5401 tagtaggagg tatttaagtt gataaaaaaa atactagttg caaatagagg cgatatagca
     5461 gttagaatca tcagaacatg taaagaacta ggtataaaaa ctgttgctat ctattctgaa
     5521 atagataaag attgttttca tagatatata gctgatgaat ctatttgtat aggaccaaat
     5581 aatataagta agagttataa taatatagaa aatataatct atcttgctct taaattaaag
     5641 tgtgatgcta ttcatccagg ctttggattt ttatcagaga atcctgaatt tgcaaagcaa
     5701 tgtgaagata ataatattat ttttataggt ccaacaagag aacaaatgat tttgatggga
     5761 gataaatcta gagcaagaga aactatgatg gaattgaata tccctgttgt tcctggttca
     5821 gaatctgttt tgaaaactaa ggaagaagct ttagaagttg caagagaaat tggatatcct
     5881 gttatgataa aagcctcaag tggaggcggt ggaaaaggta tgagaatcgt tagaaaagaa
     5941 gaagaacttt tttcaaattt tgatatggca agttcagaag ctctagcagc attttctaat
     6001 agtgatttat atatggaaaa gtttatagaa aatcctagac atatagaagt acaagtattt
     6061 ggagataaac attcaaatgc aatacattta ggtgatagag attgttctat gcaaaggcga
     6121 aatcaaaaag taatagaaga atctttaagt ccataccttt cagatgaaga aagacaaaaa
     6181 cttcataaaa ttgcagtaga tatagtaaaa ggagtaggtt atatcggtgc aggtactatt
     6241 gagtttatag ttgacaaaga taaaaacttt tattttatag agatgaatac aagaatacaa
     6301 gttgaacatc cagttacaga gatggtaaca aatcttgacc ttataaaact tcaaatatct
     6361 atagctaatg gggataaaat tccttttaaa caagaagata ttacttttag aggtcatgct
     6421 attgaatgta gaataaatgc agaagattca tcaaagaatt ttgctccatc accaggtaag
     6481 atagaatcat taaatttacc tggaggtttt ggtgttagat ttgatacttt tgtatatgca
     6541 ggatatacta taccaccttt atatgattct atgataggta agttaatatg ttgggctgaa
     6601 actagagaag aatgtataag tagaatatat agagctctag acgaaattat tgtagaaggt
     6661 attaatacaa atgtagaatt tcaaaaggca cttgtaacaa gtgaggagtt tagagaagat
     6721 actcatcata ccaaatttat agaagatgtc tttatgaaga aagaatttgc tacattataa
     6781 ggagtaaata gattatgata aaaaaatttt tatcatcaaa agaatctaag tatgttacga
     6841 tttctttaga tgacaacttt aaaaagaata gtgtagatga taaattttgg acctattgca
     6901 aaggttgtga tagtcatgta tttagaaagg acatagaaga aaattcattt gtatgtccaa
     6961 aatgttcaag acattatggc ttaagagcaa gaaaaagaat aaatttattg attgataaag
     7021 gaacttttat ggagtttaat tctgatgtag aatttcaaaa tccactaaat tttcctaaat
     7081 acaaagaaaa agtagactct tataaggaaa aaacaaaaga gtcagaagct gttatcacag
     7141 gttatggaag aataaatgga attaaaacag ttatttgtgt aatgaatcct gattttatga
     7201 tgggaagtat gggttctatt gttggggaaa aaataactta ttcaatagag tatgctgctg
     7261 aaaataattt acctataatt atttgttctg cttcaggagg agctagaatg caagaaggta
     7321 tggtatctct tatgcagatg gcaaagactt ctcaagcact ttctaagctt gaagaaaaat
     7381 cattgccata tatatcagtt ctaactgacc ctactactgg aggagtaact gctagttttg
     7441 ctatgcttgg tgatattata atttctgagc caaatacgtt gataggtttt gcaggtccaa
     7501 gagtaataga gcaaacaata aatcaaaaac ttcctgaagg atttcaaact tctgagtttt
     7561 tacttgaaaa aggttttatt gacatgatag tagatagaag aaaaatgaag gaagtattat
     7621 atcaaattct tgctatgcac aagaaatagg gcataaagga ggaactgttt tgattgaaaa
     7681 taatagtgaa aaaataaaag tcttagaaaa tgatattaaa caattaataa ctatttctaa
     7741 ggcaaataat atagatttaa gtgataaaat aaattcactt aatgagaaat tggaaaagct
     7801 taaggaagat gctttttcac atctatcagc atatgaaaag gtaacactaa gtagagatat
     7861 aaagagacct acgacattgg aatacataga acatatttgt tctaattttt tagaacttca
     7921 tggagatagg ctttttaaag atgacccttc tatggttgga ggaattggtc agataggaaa
     7981 gtttaatgtt actatagttg gacaccaaaa aggaagagat acaaaagaaa atataaaaag
     8041 aaattttgga atgcctcatc cagaaggata cagaaaagca ttaagactta tgaaacaagc
     8101 tgaaaaattt aatagaccta taataacgtt tattgataca tcaggagctt tttgtggtct
     8161 agaagcagaa gaaagaggac aaggagaagc tattgcaaga aacttacttg aaatgagtaa
     8221 actttctgtt ccagtaatca cttttgtaat tggtgaaggt ggtagtggag gagctttagg
     8281 tatcggagta ggaaatgatg tttgtatgtt agaacattca gtttattctg taatttctcc
     8341 agaagggctt tctagtattc tatttaaaga ttcatctaaa gctaaagaag cttgtgatgt
     8401 tatgaagctt acaagtaatg atttatatga tttaaaaata atagataaga ttataaaaga
     8461 acctcttgga ggagcacaaa aagatgtaga agctgtttca aaagaaataa aagcttacat
     8521 tttagaaaga ttaaatcatt acaaagatat ggataaagag gaaattatag ctcaaagata
     8581 taaaaaattt agaaatattg gaaaatgctt atagaatata aagaaatttg tagttttttc
     8641 ataataaaat ggttaaaaaa aataaaggtt ttcttaaaac gattatagaa gtatattttt
     8701 ataaaagtat actttaaatg atgattgtaa ttagtaaatg gcaattatga tttagtactt
     8761 taggtaatgg atactttaac aaattaacaa ggagatgttg aaatggaggt actaaaagtt
     8821 tcatcaaaat ctaatcctaa ttctgtagct ggagccttgg ctggagtact aagagaacga
     8881 ggcgttgcag aaatacaagc tataggagct ggagctctaa atcaagcgat aaaatcaatt
     8941 gcaatagcaa gaggttttgt tgcacctagt ggaatggatt tagtttgcat tcccgcattt
     9001 actgatatag aaatcgaagg tgataagaaa acagccatta aacttataat tgaacctaga
     9061 taatatatct ctttattaga atttaattaa gttttaaagg ttggctatag aaataggaca
     9121 ctatagtgaa ccttttttac tttatatgtt ttattttatg taacttttaa tgtataatct
     9181 taagtaaaca aataagattg gaggaaatat acattatgaa attaaaatta ttggtagaag
     9241 agtatgcagt atgtagatta aataatgaca gtaaaatacc aacatggata gatacagaga
     9301 agttttattc tataactaga actgatgatg aattaagtgt tgtatgttta aataataata
     9361 ttccttctga tgtcaagtca gaaaaggagt ggagaatact taagatacta ggtcctttag
     9421 acttttcact tgtaggaatt ttatctaagt taagtagttt gttagcagac aacaaaataa
     9481 gcatatttgc aatttctact tatgatacag actatatctt agtaaaagaa aaagatatag
     9541 aaaatgcttg taaaatttta agctgtaatg gatatgaagt agagtagtta aatactttta
     9601 catgaggact taacttatga tttatataat cgaaatttta aaaataagtg taataaatat
     9661 tttttgtttc ataggaatat ttattgtatt tggattgatt tttagcatta cagaaaatct
     9721 aaataataaa tttatatttt catcttttgg aaaaactgga attattgtaa ctggagctat
     9781 aggaacgttt tttcatgaat tgagtcattt gataatgtgt ttgattttca tgcataaaat
     9841 aaattcagtt aaatttttca gaccaataga aagtaaaaat gatggtatac taggatatgt
     9901 aagtcatagc tataaaaaag ataatcttta tcaaagtata ggaaatttct ttataggaat
     9961 tgcacctatt ataggtggaa caataatgat tattttaatt tttaagatat tactgccaga
    10021 tagttatatg aaagtgactc aaaatataga tttaaagtta tatgtaagta tgattaataa
    10081 tttaaatata aatggtttta tacacattat tttaggtgat atttccaatt ttataagaat
    10141 aatgttgtta acacctagtg tatattcatt gaagtatttt atatttatgt ttttaatgta
    10201 ttctatatct acacatatgt cacttagtag tgctgattta aaaaatagct taaatggtct
    10261 gagctttata gttatagtta tttttgtaat aagttcaatt acatacttat ttggatttga
    10321 taacttgaat atatccagtt taatagttaa gtataacata tttataagtt tctttatggc
    10381 aatagggttt attttttcaa tagtaactct cataatatcc tatattttaa gcttagtcag
    10441 cccatttaaa aactttagtt aaataaatac atattttact aatatttggt ttcgttagaa
    10501 aca
//
LOCUS       DAELJO010000138        10473 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELJO010000138 DAELJO010000000
VERSION     DAELJO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.138"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..689)
                     /locus_tag="KPB88_002776"
     CDS             complement(<1..689)
                     /locus_tag="KPB88_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055042.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKI"
     gene            complement(704..1465)
                     /gene="pgeF"
                     /locus_tag="KPB88_002777"
     CDS             complement(704..1465)
                     /gene="pgeF"
                     /locus_tag="KPB88_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG0055043.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(1462..1923)
                     /gene="nrdR"
                     /locus_tag="KPB88_002778"
     CDS             complement(1462..1923)
                     /gene="nrdR"
                     /locus_tag="KPB88_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG0055044.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(1945..2208)
                     /locus_tag="KPB88_002779"
     CDS             complement(1945..2208)
                     /locus_tag="KPB88_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0055045.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(2312..3085)
                     /gene="sigG"
                     /locus_tag="KPB88_002780"
     CDS             complement(2312..3085)
                     /gene="sigG"
                     /locus_tag="KPB88_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG0055046.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(3177..3899)
                     /gene="sigE"
                     /locus_tag="KPB88_002781"
     CDS             complement(3177..3899)
                     /gene="sigE"
                     /locus_tag="KPB88_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG0055047.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(3924..4796)
                     /locus_tag="KPB88_002782"
     CDS             complement(3924..4796)
                     /locus_tag="KPB88_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG0055048.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(5086..6360)
                     /locus_tag="KPB88_002783"
     CDS             complement(5086..6360)
                     /locus_tag="KPB88_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0055049.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(6644..7801)
                     /gene="ftsZ"
                     /locus_tag="KPB88_002784"
     CDS             complement(6644..7801)
                     /gene="ftsZ"
                     /locus_tag="KPB88_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG0055050.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(7991..8344)
                     /locus_tag="KPB88_002785"
     CDS             complement(7991..8344)
                     /locus_tag="KPB88_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG0055051.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(8443..9117)
                     /locus_tag="KPB88_002786"
     CDS             complement(8443..9117)
                     /locus_tag="KPB88_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0055052.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQGNNGNK
                     VNLFAK"
     gene            complement(9122..9820)
                     /locus_tag="KPB88_002787"
     CDS             complement(9122..9820)
                     /locus_tag="KPB88_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0055053.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKAIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(9843..>10473)
                     /locus_tag="KPB88_002788"
     CDS             complement(9843..>10473)
                     /locus_tag="KPB88_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG0055054.1"
                     /translation="KKIDVIGNKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNP
                     YIENVEIKRKLPNKIIISLKEKEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLI
                     VDVDYSIDDNKSIKFKNYKTKENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSN
                     IKILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         3125 a         1933 c         1016 g         4399 t
ORIGIN      
        1 tctattttgt atcctacacc tcttatagtc tctatatatt tttccgattt atcttcatct
       61 tctatttttt ttcttaaata tcttatatga acatctactg ttctagtttc tccatagtat
      121 tcataccccc atattttatc caaaagataa tttcttgaaa gtacttttcc tttattttga
      181 attaataatt ttaacaattc aaattcctta agagttaact ctatcttttc agagcctttg
      241 gtaacttcat gttttgataa atctagtttt aaattacctg tagtaagtat attgttttct
      301 tcacctaagc tagtcaaatt atatctcctt aaaacagaac taatcctagc taacaactct
      361 tttatactaa atggcttagt tatatagtca tcagcaccta tttcaagccc ttcaaccttg
      421 tcattttcca tatttttagc agtaagcatt ataacaggaa tattttttaa gtccttatca
      481 cttcttattt ttctcaaaac ttctattcca cttatatttg gtaacatcca atctaaaagt
      541 attaaatctg gtttaatttc cttagccttt ataaatccgt caaatccatc atatgaataa
      601 ctaacttcgt aatttgacac ctccaaattg aattttaata gctcaactat atgcatttca
      661 tcgtctataa caagcacttt agtattcatt aaaaagctcc tctctatttt atctgtagta
      721 ccgttcctat tcgttctgat gccttattat gttttctata agagtggaat ttatctgctc
      781 tacaactagt gcataaattt aaatttatta tattttcttt tttaactcca ctacatctaa
      841 gcatatactc attaattttc cataaatcta aatagtaact tccctctttt attatataga
      901 acttttcatc attatttgta agaattgtgt taaatttttc tactaaatct tcagacactt
      961 cataacaaca tggtccaatt gatggcccaa ttatacatac taaatcttca ggaactgttt
     1021 tatagcattc agacattaat tttattgttt tatgacctat attactaaaa gtccctctcc
     1081 aacctgcatg agccagtcct atggctttat ttttagcatc tataattgat ataggtacac
     1141 agtctgctgt aaacagtaaa agtggtactt tttccaagtt tgttataagt gcatctccat
     1201 ctcttctttt ccctatagta tctttattga ctatattaac tatatctgaa tgtatttgag
     1261 aattactagt tagattttca aatgtcaaat cacattcttt acatactttt aacatatctt
     1321 ctctattttt tgcatctata ttcttacatg ttataactag atttacagaa tttaattttt
     1381 catcaagttc ttcacaatta ccacttaaat taacatactc tttaatatct tgtacatttt
     1441 ttatatttat ataatctttc atcatttgtc tcctttttct atcagtatac ttttaagttc
     1501 atttacaaaa gtatttatat ctttaaattg tctatacaca gaagcaaatc ttacataggc
     1561 aacctcatct aactctttca atttatccat taccatctca ccgatttttt tggtttcaat
     1621 ttcttctata aaacatttat ttatttcatt ttcaatatgt acaacaatac tttcaatctc
     1681 gtcaatagaa acaggtcttt tttcacatgc ttttagcaat ccatatttta tcttttctct
     1741 atcaaaatat tctctactat tatccttttt aatgaccatt aaaggagttg tttctatttt
     1801 ctcatacgta gtaaacctct ttttacatga ttcacactca cgtcttcttc tgatagattt
     1861 taaatctgta tgtctagaat ctattacctt tgattcttta taattacaat aaggacattg
     1921 catctatatt cctcctttgt ttaactatat ctcaaactca tctaagctta attctgaacc
     1981 tatatttact attattgtat cacaacctat tttaagtata tcattccaaa atataacttc
     2041 ttcatcccct cctcttgaaa aaaaacttct acttccatct ccaaaaatgg taaatgagac
     2101 taccttacct tcgtgaatat ccatgtctat gtctattata aaccccatcc tttttccatt
     2161 ttttacattt ataatttctt tttccattat atcagaaact ctaatcatat tgtactcacc
     2221 ctttctttaa aaataaatat gtatcgaagt aaaaaaaaat gacctatata ggtcatttaa
     2281 attaaaataa tgtttacagt ccatggatta cttatacata ttttctcata ttctttaaag
     2341 catttttttc aattcttgat acttgtgctt gagatatacc gatttcatca gcaacttcta
     2401 tctgagttct tcctttgtaa aaccttaaat ctaataccaa cttttctcta ctatttaatt
     2461 tcttaatagc ttctttaagt gatatttctt gcaaccaatt ttcatcagta tcttttttat
     2521 cttgaacctg atccataacg aatattgcat ctccattgtc ttgatatact gggtcaaaca
     2581 gagatattgg gtcttgtatg gcatcaagtg ccattacaac tgattctact tctaattcta
     2641 attcctttgc tatttctgat actgtaggtt ctttagaatt tgttctaatt aatctttctc
     2701 taacttgtag tgccttatat gctatatctt ttaatgacct acttactcta attgggttat
     2761 tgtctcttag ataccttcta atttctccta taatcatagg aacagcataa gttgaaaacc
     2821 ttacattttg gcttaaatca aagttatcta tagcttttat aagaccaata caacctattt
     2881 gaaataaatc gtctatattt tctcctctgt tgttaaactt ttttattaca ctcaagacta
     2941 atcttaaatt ccctcttaca aactgttgtc tggcttcttc atcaccattt tttatttgta
     3001 ataaaagttc cttcatttgc ttatttttaa gaactggaag ttctgatgta ttaacaccac
     3061 atatttcaac cttattaact tgcatatatt ttcagtcctc tctcaaaagt attcatctat
     3121 tgaaattatt tacatttcca atttttttat actttttaag aagaattaat ttagcattat
     3181 acaaattttt tcatttcttt ttgcaacctt gagattattt tcttttcaag tctagatatg
     3241 taggactgtg atattccaag cattccagca acttcttttt gtgttttttc aataccttta
     3301 tcaataagac caaatcttaa ttccataatt tgcttttctc tgtcacttaa tctgtcaagt
     3361 gccataacca acaaatctct atctatttct tcttcaatta ttttgtatat ttcatcattt
     3421 tctgtaccca atacatcaga taataaaagt tcgtttccat ctaaatctat atttaaaggt
     3481 tcatcaaatg atacttctgt tttcttttta ttattttttc ttaagtacat taatatttca
     3541 ttttctatac atctcgaagc ataagttgcc aattttatat ttttattcag tttaaaagta
     3601 ttaactgctt ttatcaaacc tatagttcct attgaaatca aatcttctac atctatacct
     3661 gtattttcaa attttctcga tatgtagact actaatctaa gatttctttc tattaaaatt
     3721 gactttacac tttcatctgt ttctagcttt tgtaaaagct ccatttcttc ttctggtttc
     3781 aatggaggag gaagtatatt tgcccctccc atatagtata ttccctttgg ctttattatt
     3841 tttaatttaa tacctaacat agtaataaag cttattattc tttctttcaa tcgtaacatt
     3901 ccttgtcccc cttaaatttt cacctaaaat ccaggcaata tatttggatt cattattgct
     3961 ccatattctc tatcattaaa atttgaaata ccaagtatta tatttcctat cttttgctca
     4021 tctacttcta tatagtcagc ttttaagcct attattatgc ttgtcttatt acttccagca
     4081 tgtttataag gtattattct aactctacct gaagttttat catctaaact tcctataatt
     4141 tcttgtgctt tcttcatatc tatatcttca taattaatat ttaatagttt ttctggaata
     4201 aatccttcta acacactaga cttaactatt attacatcac tttgacttat tgggtctttt
     4261 aacaaattac cactatctac taaagcttta cagcaaaaac ttttttctag taagtttata
     4321 tttatagtct ttgtaagctc cttaatgtac ttaagcattt tcaaatcttt ataaatgtat
     4381 tttaataatt cacaactcac ataagtacat gcaattaaga aagatatttt taagtgactt
     4441 attcctgtaa aataaattat aaaataagtg cttccagaaa taaaaatgtt tactagataa
     4501 aaaactaacg ctacatgaac ataatctttt ttgctcttac ttctaaatga aattaaagtt
     4561 attatactca tgattattat tttaaatggt acagtaaata gtatattcat tgatggatac
     4621 aaataagcaa cagaataggc acctcctaaa ctagccccca ataacttatt ttttttagga
     4681 ttatatcttt tagtaagaat agaagtacaa cttataataa tataatttat tactaggttt
     4741 tctataacat agtactcaat atacaccatg tatcccccct taaaaccttt aaacatatta
     4801 taagatagta tgtacaaaat tttatgtcac ttttgatggt cgaatccaaa acaaattcta
     4861 tttaaaaaga acaatgatat atgtcttata ctttatgtcg tcaaaagata tttttttaat
     4921 gccaaacaaa tagacttttt tgtagaatcc aatatatcta ggtagaagtt attcgctcta
     4981 cttttggtat atttacaaaa aaagactaca taatttaata atatatcaag taaatctttt
     5041 acttatataa aatcaaatta tatagtctgt aaattcaaat ataaattatt gtgctagtaa
     5101 tccatttgca tattttgcag attcatttag tgcttgttct ggagttatgt ctccagctat
     5161 catcatttga agttgtttcc ataaatattc atatatctct actccatgag gtccaactgt
     5221 caatggtcta tacatatgtt tatctcttgt caccatttct tcaaatcttg ggtctccttg
     5281 gtatttttca cctttagcaa ctggagctct aggtatttct ttgtggaaag cttccattga
     5341 ttctttgctt agcatatact caagaagttt aaatccaagt tctttatttt tacatgctga
     5401 cattaatgtt aaatcatcta ctgaagcaaa acttccaact gtcttatctt ctaatgatgt
     5461 tacatatccc cagtttaaat ccgggaatga tttatcaaaa gtacttgtag cagcagagga
     5521 tagcattata gtaaatgcag cttttcctgg tccaaataca ctttcaatca tttcattact
     5581 atcttttgat aaagcatctt ctggaatata ctttttcata tcatttataa agtttgcagc
     5641 tttcaagcct tctgcattat taaatttaac tgattttaaa tcatcattat atatatctcc
     5701 accagcttgc cataaatatg gataccagtt ccagtttaag cttccaaata ctttagaacc
     5761 ccaaccttgt gcaagacccc actggtcgat ttttccatct ccatcagtat cttttgttgc
     5821 tttttcacag attcttttaa aatcatccca tgtttttgga ggtttttctc ctaatttttc
     5881 tagtatatct ttattgtagt acaatacgcc tggattagca gcttctatag gtaaaccata
     5941 aataccaccc atcatttttg catcatctaa gtatagataa ttatctgaag ttcctgattt
     6001 ttctaaataa ggtgttaaat cttctaccgc acccatttct ataaattgag gaaacatctc
     6061 tgcatacatg tatcctatat ctggaccttc acctgcactt attgctgttg catatttttc
     6121 tgcgtagtta tcccatggta ctatctgaaa ttcaatttca cagttatttt ctttttcaaa
     6181 tttttcaaat atcggcttcc atgcatctgc atcatggtct gttaatggtg cagaccatac
     6241 aactaatttt tctttaccct ctttgccttt agaatcttct ttttttgaac atcctgcaag
     6301 aattgatact gatagaatcc ctaccataac taaagaaaga acctttttta gtttaatcat
     6361 gcttaatccc ctttcagact ttaatttgtc tttttcctca aatgtttaca tttaattcag
     6421 atttaatatt aacaaataag attactcttg tctaatcata aaaaactata ctaactccta
     6481 taactatatt ttttttctat acctattaga tttaattcat actaaaaact tactttttaa
     6541 attttatcaa tatttaaaaa tttttttaag tctagccaca aactaactat ctaaattgag
     6601 caaaataaaa agaagtctat tgacttcttt ataagcttac atattatctt cttcttctta
     6661 ggaatgtagg tatctccatg ctctcatcat catcttcttc aattatactt gcctttttag
     6721 gaggctctat tttttcctca gcaattactt cttcttcaat ttcttttaca gcttgtttta
     6781 ctgttgtatt taatgaagaa cgtatgcttg atttaggttt agtatctaaa tctaaggcac
     6841 cattttgtaa tccttcaaat cctgtcgcga ttactgtaat cattatttca tcacctaaat
     6901 cttcttttat agaagctcca aatataacat ttgcttctgg gtcacaggat tccataacta
     6961 aagttgatgc ctcattaact tcaaataatc ctaagttagg accacctgta acattaagaa
     7021 gtactccttt agctccttgt attgatgttt ctagaagtgg agattgaata gcttgtcttg
     7081 cagcatcaat tgctctagtt tcaccactag cactacctat acccatatga gcaagtccct
     7141 tgtctttcat tatagtagta acatctgcaa agtccaagtt tattaaccct tcaactgcta
     7201 taaggtctga aattgattgt ataccttgtt ttaatacatc atctgcaact gcaaaagcat
     7261 ctaacattga tgtatttttt tgtactatct gtaaaagtct atcatttggt atcgttataa
     7321 gagtatctac ttttgattta agttctgcta ttccaccttc agcgttcttc attcttatct
     7381 taccttcaaa tgcaaaaggt ttggtaacta cacctactgt aagtatcccc atttcttttg
     7441 ccaagcctgc aaccactgga gcagctcctg taccagtacc accgcccatt ccggctgtta
     7501 caaagaccat atcagctcct tgaagtaact ttactatttc atctttactc tcctcagcag
     7561 ctcttttccc tacttctgga tttgctcctg ctccaagtcc tctagttaat ttttctccta
     7621 tttgaacttt atattcagct tttgatgtat ataaagcttg tttatctgta tttactgata
     7681 taaattcaac gccttttagt tgagcttcta ccattctatt gacagcattg tttccaccgc
     7741 cacctacacc tattacttta atttgtgcac attcttctaa ttctacgtca aagtttagca
     7801 tcataaaccc ccttctctca tgattcaaag ttatcgtatg tattaattat atcattttct
     7861 tcttttattt tgttaacctt taagtaacat attccacaaa aacgtaaatt ttccttttta
     7921 aatttcttac ttatattgtc aaatattact ctttaagtat aaaataattt acgtatatat
     7981 taaatgctct ttaattttta cgttttttgt tcataatatt actaataata tatcgtctaa
     8041 ttatagataa gttattaaac aatctcatac caaaaactaa tacagcaaca tagtaaagag
     8101 aaactcctaa cttttctcct atataagtca ataatatagc caatatagca ttacctatga
     8161 aaccagtaat aaaaattaca ttatcatagt tatcttcaaa attggatttt atcgctccaa
     8221 aaatagaatc cattattgca agaatagcta ctgacatata tatggaataa tccatagaat
     8281 atgtaaatgg aatatagtaa cctactacta caccaaatgc taatcctcct aaaacccata
     8341 acataaatct tcacctcttt attttacttt gaaacacaac tacattacaa tcctttttat
     8401 taattttttt tactttttct ttattttttt gtatttttcg ttttattttg caaataaatt
     8461 cactttattt ccattatttc cctgaacttt tatattaatt ccatatatat ccttaagaga
     8521 tttagcatat gattctggag agtttatagc tgcactcaat aaatctatat ctcctatagc
     8581 ttttattata aatggttgtc catatgtagt atcatttact gtaatagtag ctcctgaaca
     8641 ttttatctta cttgttgcta gtactctttc tccatttata gaaattgctc ttgctccagc
     8701 cttttttaaa tcatttaaaa ccctcaaaat atctatatca tgtataatca aatcgtttgg
     8761 attctgtccg tcttttaact ctctctcact atccctcata gtaatagtta tgcctggccc
     8821 tgtaacagtt gaataaccac ttaactcttt aacttcttca agctcttttt gcataagtgt
     8881 ttggatagat tcatttccat tcttttttgc ttctttatat tcattcaaag tttttttgtt
     8941 ctttttaaga gtttctttta aattttttat ttcttttttt gtattttcta cctcactttc
     9001 aatactttca gtttgaccta atgttatgta tactctattt gggtctagtg attttatata
     9061 agttgatatg aaaacgccta atatgaatgc tcctaatata actattttct ttttcatact
     9121 attatttccc ctctgctttg tttatatcac tatgaccatt tatttctata ttgtcttctt
     9181 tggtcacttt tactttggca ttataatatg tttctatttc ccagacaata ccatacttta
     9241 tattaagtgc tgaagccaat gtatcagaat caccaattgc ttttattata attggttctt
     9301 ttatgacagt gtcattcata taaagcttgt cttcttttaa atacatatat gtattagaaa
     9361 ctattctctg gtcgtttata gatatggcac ttgcttgtgc tgaatttaat ttatttatca
     9421 ttgacaataa taaatcataa ttatatagta tactatcatt tttatcagag tttcctaagt
     9481 cttttgtaga atttatctca atttttatgc ctgttccttc aacatctgta tatccagcta
     9541 atgtttcata tttttctatt tctgacttaa gtatgtcttg ttctccttca ttgtttttgc
     9601 ctctataagt ctctatagct ttcttcacac tattaacttc tgattgtaga ttctcttttt
     9661 ctttttttaa tgatttaagt tcttctgtaa gttgctctcc tcttttaaaa gttgtcatcc
     9721 ctctattttc tatattaata attttaagtt gtaaactaac aagaacaccc aatacaatac
     9781 ttcctatgat tatactttta tttataaatc tttttttcaa tatatcactt ccttacaatt
     9841 gtctatcctt ctggactata cactgccaac tttccatagt taagatttat agtccctcca
     9901 ttagtatttt tattttgcag gtcaatcaga attttactta ccctagatat attataatct
     9961 aagttatcat cattagaaag taagattttt atattacttc ttgtaagcat ttctatattg
    10021 ctttcttttt ttaagtttac ataatttatt tttctataaa ttccttcttc ttttaaataa
    10081 tttaaagttt taaatacatt ctccttagtt ttataatttt taaattttat agatttatta
    10141 tcatcaatgc tataatctac atctactatc aagtcttttt tactttcatt agaacctctt
    10201 agttcttcca ataaatttcc ttttttatct atataacaat aattatcttc atcttttaat
    10261 actgcaaata tttctttttc ttttaaactt ataattatct tattgggaag ttttcttttt
    10321 atttccacat tttctatata tggatttttt ataagtttat ttttcatatc tttaaaattg
    10381 tatgcaaaaa tattttcatt tagatttaca tttagttctt tcattatatt tgatttggtc
    10441 accctcttat ttccaattac atctattttc ttt
//
LOCUS       DAELJO010000139        10468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELJO010000139 DAELJO010000000
VERSION     DAELJO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.139"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            325..1749
                     /locus_tag="KPB88_002789"
     CDS             325..1749
                     /locus_tag="KPB88_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG0055055.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     SPFVKRASANQSEEESTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1784..3913
                     /locus_tag="KPB88_002790"
     CDS             1784..3913
                     /locus_tag="KPB88_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0055056.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KNLSELIEDSSRIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3943..4365
                     /locus_tag="KPB88_002791"
     CDS             3943..4365
                     /locus_tag="KPB88_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055057.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4384..5535
                     /locus_tag="KPB88_002792"
     CDS             4384..5535
                     /locus_tag="KPB88_002792"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0055058.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5641..5742
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5925..6497
                     /locus_tag="KPB88_002793"
     CDS             5925..6497
                     /locus_tag="KPB88_002793"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG0055059.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6561..7211
                     /gene="fsa"
                     /locus_tag="KPB88_002794"
     CDS             6561..7211
                     /gene="fsa"
                     /locus_tag="KPB88_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0055060.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7363..10287
                     /locus_tag="KPB88_002795"
     CDS             7363..10287
                     /locus_tag="KPB88_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0055061.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4295 a         1019 c         1952 g         3202 t
ORIGIN      
        1 gtgtataaac ctcaaaaata cagataaatt aaatctatat tagtatatcc aaaagtacat
       61 ttaataaatt tatttaactg aattattatt ttcatttcta ttaaatttaa atattttata
      121 aaaatagtat tatttacaaa atcatatact ccaaaatact acataaataa aattaaacaa
      181 aaactaaaat gacaaatgtc attatctaaa gtgacataga aacggtttct taaagttgtt
      241 actataaaat ataaactcta taataaaagt ataacaaaag ggaatcatat cgatatagaa
      301 tataattcaa gaggaggtaa aattatgagt agttctaatc aatcgacaat aaagacatct
      361 atacagcgag tagggaaatt tttaagtggt atggtaatgc caaatatagg agcttttata
      421 gcatggggtc ttataacagc attatttata ccaacaggat ggatgcctaa tgaaaaattg
      481 agtactatag gtgacccaat gataaaatac cttttaccac tattgatagc ttatactggt
      541 ggtaaagcaa tagcaggaca aagaggtggc gtaataggtg cagcagcagc tatgggagta
      601 attgttggtg cagatatacc aatgtttatc ggagcaatga taatgggacc atttgcagga
      661 tgggtaatta agaagtttga taaatttgta gatggaaaga taccaacagg atttgaaatg
      721 ttagtaaata acttttcaat aggtataata ggtatgcttc ttgcaatact aggattttat
      781 gcaattggac cagcaatagt agcagggaca gctcttatag aatctggagt tcaatttata
      841 gttagcaaaa gtttattgcc tctagtttca gtatttatag aacctggaaa agtattattc
      901 ttaaacaatg caataaatca tggtatatta gggcctatag gtatagctga ggcaaaagaa
      961 gcaggtaaat ctataatgtt cttactagaa agtaaccctg gaccaggttt aggagttcta
     1021 ttagcatatt ggatgtttag tagaggaagt gtaaaacaat cagcaccagg agctgtaata
     1081 atacatttct ttggtggtat ccatgaaatt tacttcccat atattttaat gaatccagtt
     1141 ttaatattgg ctacaatggc aggtggagca gcaggaatat tgactttttc aatattagga
     1201 gctgggcttg tagcagcacc atcaccagga agtatatttg cacttatggc actagctcca
     1261 aagggaggat tacttccagt attagcagga gttgcagtgg caacagttgt atcattctta
     1321 gtggcatcac catttgttaa gagagcttca gcaaatcaaa gtgaagaaga aagtacttct
     1381 ttagaagaag ctaaagcaaa aatgagtgat atgaaatctg caagtaagaa ctctgagaaa
     1441 aatattgagg aaaaacagct tgaagtaaat gagattaaga agattgtatt tgcatgtgat
     1501 gcaggaatgg gttcgagtgc aatgggagct tccagattta aaaatagaat aaaaaactta
     1561 gacttaaata tagaaattac aaattcatct gttgataatc ttccagatga tacacaaata
     1621 gtagtaactc acaatacatt agttgaaaga gttgctaaga ataattcaag tgttgagata
     1681 gtttcaataa acaatttttt aaatgaccca aatttagata cattgtttaa aagattagaa
     1741 tctaaataaa aataaaaagt accactaatt aggagtggat aatttgaaaa aaaagaagat
     1801 tacatctagg caaaaaaaga taatactaat gatagtagaa aattcaaaga aaaacattcc
     1861 aataactatt tctgagattg cagggacttt agagttaagt tcaagaactg tacttagaga
     1921 tatgtctgga attgaaaaat ggtttgatga aaatgatttt aactttgtaa aaaaaccagg
     1981 agtgggattg attttagagg aaaatataga aaatcaaaac tttataattg agttgttaga
     2041 agaagaaaaa atagaaaaag aatacagcaa ggaagaaaga aatttaatta tattatcaaa
     2101 gctactagtt tctaacgagc cagtaaaatc ctattatttt gcaaaaatat taaaggtatc
     2161 tgaaggggta cttaataatg attttgcctt agcaagtaaa tggttggaaa gatttgatat
     2221 tgagcttgtt agaaaaccag ggcttggtgt ctacttaaaa ggtcaagaaa aaaactttag
     2281 agaggcatat gtaaatctta tatacgattc attcaatgaa aaagaaatac tagatatggt
     2341 cagaaatata agtgaaaata tacaaacaga taaagcaata gaaattttaa gtgaaaatag
     2401 acttttaaat ttaatggaca ggtgcataat aagaaaagtt gaagctacat taacaaagaa
     2461 gctttcagat ttagatgtaa atttagctga tagtgcatat atagggcttg tagttcatat
     2521 ttcattagcc ttacagagga taaaaaatgg tgaaaatata actatggata aagagtttct
     2581 aaaagaatta tctataacag aagagtttaa attagctaaa gaaatcgtaa aaggtatgga
     2641 aacagatttt aacatggata taccagtaga tgaggttgga tatataacca tgcatataag
     2701 aggtgcaaag caaaggtcat cttcaaacca taaggcttta aacttagatg atatagaaat
     2761 aatggaaatt acaaataaga tgatagattt ggcggaagat gaatttaaaa taagtttaaa
     2821 aaatgacgag aggttattta aagacctagc aaatcactta gggccttcta taaatagatt
     2881 gaatatggga ttagaaataa gaaatcctct tttagatgaa ataaaaagca agtattcata
     2941 tgcctatgat ggagttgaaa agatttctag aattataaaa gataaactaa atataaattc
     3001 aatacctgaa tcagaaatag gatatattgc aatgcacttt gcatcagcca tagaaaaaaa
     3061 tcttatgatg aatactaata taaatattgt tgtggcatgt cccactggaa taggtacttc
     3121 aagattctta tcaactaaga ttgaaaataa atttcctaat ttaaatatac ttgaaaccat
     3181 atcagctata aacatagatg aagagtattt aaaggaaaaa gatgttgatt taatagtgtc
     3241 tacagttgag ttaaatacaa gtttaaatta tatatgtgta ggtccattta tgagtttaga
     3301 tgatgaacag ataatcaagg aaaaaataaa atctatagct caaaataaat taatcaattt
     3361 aaatgcgaaa aatgatacta agagtaagaa taaagtgtat gaacagataa cagaaagtat
     3421 gaatatagga aaagatattc ttcaattttt agaggaaatt agatttgaaa aatttgaaag
     3481 taaaaattta agtgagctta ttgaagactc atcaagaata tttgcaaaat ctagtgaaga
     3541 tataatctct ataaaagaat cgttaaaaga aagattaaaa atctcaattc catatataga
     3601 agaatcaaaa attctattgt tacattgtat gagtgaaaga atagatatta tgaagttatc
     3661 aataataaaa ctagagaata gaatcgtgct agattcaaat gaagaaatcg acaatgtagt
     3721 ttttatgtta cttcctaaaa attcaccaag ttatcaaaga caaataatga gtgaaataag
     3781 tggttcatta atagacaata taatttttac taataagata aataaattct ctatagaaga
     3841 gatggcatta gaggtaaaag atatagtttt taatttttat acaaacagat taaaagcatt
     3901 tatagataaa taaaattaat attaaaaagg aaggttgata ctatgagtaa agtattgaat
     3961 gagaataata tatttttagg gttagatagt gtttcaaagg aagaagctat aactttagca
     4021 ggaagaaaat tagttgaaaa tggatatgta aaagaagaat atataccagc aatgttagaa
     4081 agagaaaaag taatgactac atatatggga atgggagttg ctattccaca tggtgtaaat
     4141 gaagcaaaaa aagaaatatt atcttctgga atagttatat tacaattccc taatggtata
     4201 gattttgatg gagaaaaagc atatttatta ataggaatag ctggtgttgg agatgaacat
     4261 ttagaaatac tatctaatat agcagtagtt ttagatgatg acttaacaga aagactaaaa
     4321 aattcaaatg acaaacaagc ttttatggaa gcttttgcca actaatctta ggagggattt
     4381 aaaatgaaaa aggcaattca gtttggagca ggaaatattg gaagaggttt tattggaggc
     4441 ttgttagtta agtcgggtta tcatgtagta tttgctgatg taaatgagga tatattaaac
     4501 tcaataaaca aagataaaaa gtatactata cacataagag atgtagaatg tatagatgaa
     4561 gtaattgata atataagtgc ggtttcatct ataaaagaag aaataataga tgagattgta
     4621 caggctgaaa taataactac agcagttgga cctttagttt tgactaaaat agcctctaca
     4681 attgcaaaag gtataaaggc tagaaaagaa aagggattaa caagtaatct aaatataata
     4741 gcatgtgaaa atgcaatata cgcaagttct tctttaaaag aagaggtact taagtattta
     4801 aataaggaag aagtagaata tttagagatg tatgttggtt ttccaaactg ttcagttgat
     4861 agaatagttc ctcctggtaa aaacgaaaat cctcttgatg taacagttga aaatttttat
     4921 gagtggaatg ttgaaaaaca aggatttaaa ggggaaatac caactatagt tggaatgaat
     4981 ttagctgata atcttatggc ttatatagaa cgtaaattgt ttacactgaa tacaggtcat
     5041 gctataacag cttatatagg atatttaaaa ggatataaga ctatagaaga gagtataaaa
     5101 gataaattta tctgtgatat agtcaaaagt gcaatggtag aaagtggaga aggattgatt
     5161 aaaaaataca attttgactc agaagtgcac tataaatata ttgataaaat tttaaataga
     5221 tttaaaaatc catatcttaa tgatgatgta ttaagagtcg gaagggaacc attaagaaaa
     5281 ctaagtgata aagatagatt gattaaacct ttaatgacag caaaatcata tggattatca
     5341 gttgataatt tgattcttgg aataggtgca gcgcttcatt ataataatag tgaagatact
     5401 caaagtgtag aattacaaga gttgataaag tctattgggg taaaaaaagc cgttgctaaa
     5461 atagctaata taagcaatga cgaagaactt ttaaataata tcgaaaaaag ttatatattt
     5521 atgaaaaatt tataaattta cttgaaaaaa agtagttctg ataaagtaac tataatatat
     5581 tattctacac agtttgaatt atttgttgaa taatagagaa ttataatata taattaaaag
     5641 gaataaatta atatatttac ctgtatatgt ttgataatat ggttcaaatg tttctactaa
     5701 gctaccatta atagcttgac tacaggagtg aagaagatat agtttatact tattttaaat
     5761 tatatattaa gttaattact ttgaatttta agctcctgtc tatgatatag acaggagctt
     5821 ttttagtatt ttaatataaa atacttacaa gaatagatag caaaattaga acaaatataa
     5881 tttttttatt aatataattt tagaaagggc taggtggaag ttcaatgaag gctttacaag
     5941 aaaagatatt gagagaaggc agtgtttcag gaaatgatat actaaaagtt gatagttttt
     6001 taaaccatca aattgatgta gcttttttaa atgaaatagg aagagaattt aaagagagat
     6061 ttaaaggaga aaaggtggat aagatattta caatagaggc ttcaggaata gctatagcat
     6121 caattgtttc acagtatttt gataatgcac ctgtagtatt tgcaaaaaaa tcagaatcta
     6181 aaaatttgga taaagatgta tatgaaacta atgtatattc atttactaag gcaagagagt
     6241 attcagttaa agtttctaaa aagtacataa acaaaggcga aaatatattg atagtggatg
     6301 attttttggc aaatggaaga gctgctcttg gacttaaaga tttaattgag caagcagaag
     6361 ctaacttagt tggagtagga attgtcattg aaaaaggatt tcaagctgga ggagcacttt
     6421 taaaggctaa tgatgtaaga ttagagtctt tggcagtagt tgaatctata gataatggaa
     6481 ctgtaaaatt taggtaattt atgatattat atacttgtgt ttagcacata ttttaactat
     6541 gtaattatgg aggatttttt atgagatttt ttatagacac agccaatatt gaagaaataa
     6601 aagaagcaaa tgatttaggt gtaatctgtg gtgtaactac taatccgtct ctaatagcta
     6661 aagaaggtag agattttata gaggtagtta aagaaataag tgaaatagta gatggaccaa
     6721 taagtgcaga agtaataagt ttagaacata aaggtatgat agaggaagca gaaaaattat
     6781 ctaagataca taaaaatata gttataaaga tacctatgac agcagaaggt ctaaaagcgg
     6841 tgaaagtact atcaagtaaa ggtataaaga ctaatgtaac gcttatattc tctgctggac
     6901 aagctctttt agctgctaga gctggagcta catatgtaag tccttttgtt ggtagattgg
     6961 atgatatatc acaaaatggt cttgatttaa ttgaagaaat cgttgatata tttagtgtaa
     7021 atagtattga ggctcaaata atagttgcta gtgttagaaa tccaatacat gttttacaag
     7081 ctgctagaat gggagcagat atagcaacag ttcctcttaa agttataaac caaatgatta
     7141 agcatccttt gacagacaaa ggaatagata gctttatgaa ggattgggaa ggtgcttctt
     7201 taaaattata attataaact acgctttata agtatattaa agaggttatt atggaaattc
     7261 taagtaataa ggctaggcta ttttgcctag tcttattttg gtgtataata tattgaaaca
     7321 tattattgaa atagattaat tttttaagtg aggaggcgaa aaatggataa aatattaaga
     7381 gagatacaaa gagaagataa aaaaaatcca tatactgacc aagaacttgc tgaaatgtta
     7441 aatgtagcta ggtctgaagt gatatcagtt aggaaaaaaa ataatatttt agattcgaga
     7501 gaaagaagaa aaaaaatatt aataaaagat atatcaaaaa ttgtaagtga aaatcctcaa
     7561 atgtctgaaa gaaaaataac tgaaatatta atagaaaatg gatataatat atcgagaagt
     7621 gcagtttcaa aattattaaa agaggaaaat ttaaatcagt caaagattgg gattaaggat
     7681 aaagacccag catcatcagg aaagtatgtg aaggtgagta aagttgtaga agaagatgga
     7741 tttgaagaat tgataggtat aaagggaagt ttgaaagaaa aggtaaatct agctaagtca
     7801 gcaataatgt atcctcctaa tggccttcat actataatat atggagaaac aggagtaggt
     7861 aaaagtgaat tagctacatg tatgtataaa tatgctataa aaaacaacat taaagaagag
     7921 aacagcccat ttatagtgtt taactgtgca gactatgcag aaaatcctaa tctattgata
     7981 gctcaactct ttggggtagt aaaaggtgca tatacaggag cagatgcaaa tagagaaggt
     8041 cttgtagaac aagctaataa tggaatattg tttttagatg aaattcatag attgcctgct
     8101 gctgggcaag aaatcttatt ttcattaata gatagaggag aatttagaag gttaggagaa
     8161 agtagttcta atagaaaggc aaatgttctc ataataagtg ctacaacaga aaatccagat
     8221 tctaatttac ttcaaacatt tagaagacgt ataccaatgg taataaattt acctaatatg
     8281 agtgaaagac caaaatctga aagatatgaa ataataataa agttttttga aagagaagca
     8341 aagcgagtaa acaagaattt tataataact aaagaagtta tgtgtgcact tatgaattat
     8401 aagtgtactg gaaatatagg tcagttaaag agtgatatac aagtaacttg tgctagagct
     8461 tttagtaaat cgatattttc tagcgataag gtaattatag atttagatag tttaagggat
     8521 tatataaaaa gtggatatta tgattgtaat tgtaaaaatg aaaagtattc tgagttttca
     8581 gttgaggata tatatataga tattagtgaa ttggtgagtg gaaaaaatag aattttatct
     8641 gataatgaga taagcgagat atataattat gcagaaaaag agcttaaggt tttggaaagt
     8701 aagtgctttt cagaagaaga gttaaaaaat gcatttatag aaaagttaga taagaagttt
     8761 aatgctatta aaaacaataa aaatttatct gatagaagaa gtcgaataga ctggggagga
     8821 caattaaaag agtctacact ggagataatg gataaggtaa ttgtatttat taaggagcag
     8881 tttaagcatg taaatcaagg actgtacttg gctctagcaa ttcatattga gcatgctata
     8941 ggtaggataa aagatggaaa aactataata aatccgagtt tagataaaat aaaagcatca
     9001 atgccaatgg aatatgagtt gtcaagatat ataatgggta tagtagaaga tttgacacaa
     9061 gtaacatttc cagaagatga gcttggatat ttggcttatt atataaataa attttgttat
     9121 aatgaggaaa gtataaaaga taaagttaaa gtagtaatag ttacgcatgg taaagttgga
     9181 attgaaatgt ctaaagttgt aaatcatatt ttaggtatag aatgtacact aggtatagaa
     9241 atagcactta cagattcacc atcagagggg atagagcatg tattagaaga gcttcaaaag
     9301 atagaagcta gaaaaggtat tctagttcta attgatatgg gttctttggt aattcttggt
     9361 gatgaggttg aaaaaaggct tggaattaga tgtaaaactg taaatagagt agatacatta
     9421 ttggcaatgg aagcaggaaa acttgcaacc attgaaggga aaagtttaga tggtataata
     9481 gctgatttaa agaaaaataa gaattatgct atgataaata caaataaatt tagttacaga
     9541 aaaaacgagt atggaaagaa aaatgtaatc attactttat gtttgagtgg tgtaggtaca
     9601 gctttaaatt taaaagagca tatagaaaaa caaattaagg aatatgacac tttaattgaa
     9661 gtaaagccag ttgctttttt aaataataat ttagaagaag agttaaatga tattgaatct
     9721 gaatataata ttattgctat ctgtggaact attgatatag attataaaaa cgtgcctttt
     9781 atttcttata atgaggtact tggtaaaaat ggtattaata aagtattaga gcatttaaat
     9841 agtaaacaac cagctttaaa tattgaaaac cagttgaata atttaataca tgaagatttg
     9901 attttataca attttgaagg aatttcaaag gagtatataa ttgatactct tgtttcaaaa
     9961 cttgaagaag gtggatatgt tgactcaaag tatatattaa gtgtatataa aagagaagca
    10021 atgggaagtg ttgtaatggc ctctaaggta gccgtgccac atggacttcc agaaaacgtt
    10081 ataaagcctg caatagcaat tgcgaggttg aataaaccta tagtatggga caataaattt
    10141 atggtggatt tggtggtact actggcactt aaagaaaata ataagaaaga aatacgaagc
    10201 ttattttcaa agattaatga ccagcatact ttagaagttt tattaaatac tgaagataaa
    10261 aatgaaataa aagaactttt aagctaatta ctagtaccaa aaagaaagac aaaaaaattt
    10321 gctttagtat aaaatttaaa actactcaat tgctaagtag ttaattactc tatataaggc
    10381 tagttctatg aaatttattt atatgatttt atagaattag cttttttctt ggtaaataaa
    10441 atatatgaat ttatttatgt aaaaagta
//
LOCUS       DAELJO010000140        10269 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELJO010000140 DAELJO010000000
VERSION     DAELJO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.140"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            162..929
                     /locus_tag="KPB88_002796"
     CDS             162..929
                     /locus_tag="KPB88_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG0055062.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     TKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1147..1360
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1614..2648
                     /locus_tag="KPB88_002797"
     CDS             1614..2648
                     /locus_tag="KPB88_002797"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG0055063.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2689..3906
                     /gene="argJ"
                     /locus_tag="KPB88_002798"
     CDS             2689..3906
                     /gene="argJ"
                     /locus_tag="KPB88_002798"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG0055064.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3921..4781
                     /gene="argB"
                     /locus_tag="KPB88_002799"
     CDS             3921..4781
                     /gene="argB"
                     /locus_tag="KPB88_002799"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG0055065.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4811..5998
                     /locus_tag="KPB88_002800"
     CDS             4811..5998
                     /locus_tag="KPB88_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG0055066.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6107..7129
                     /gene="argF"
                     /locus_tag="KPB88_002801"
     CDS             6107..7129
                     /gene="argF"
                     /locus_tag="KPB88_002801"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG0055067.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            7256..7873
                     /locus_tag="KPB88_002802"
     CDS             7256..7873
                     /locus_tag="KPB88_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG0055068.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8093..8791)
                     /locus_tag="KPB88_002803"
     CDS             complement(8093..8791)
                     /locus_tag="KPB88_002803"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG0055069.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8953..9342)
                     /locus_tag="KPB88_002804"
                     /pseudo
     CDS             complement(8953..9342)
                     /locus_tag="KPB88_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(9448..>10269)
                     /locus_tag="KPB88_002805"
     CDS             complement(9448..>10269)
                     /locus_tag="KPB88_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0055070.1"
                     /translation="FLNNKTKETYTQNGEERRYTRGNLKAIYGVLNSALKSAVYPYKL
                     IKENPAQYTNIPKNIIDTKKDSENKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMR
                     KGEILGLCWDNIDLDNNIIKVRKNLIKRKVSEFELTSPKTKTSIRDIRIGDTLSKVLK
                     EEKLNQKKQKIKLGKWYTETEYDWVCRKKDGSFVNHNNIDACIRTINKKLNLDFNFHC
                     LRHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILENLVR"
BASE COUNT         4015 a         1054 c         1750 g         3450 t
ORIGIN      
        1 tatttaatta tatatacaat ttaataattc agataatctt aaaataatat tatgtaaaat
       61 aaaaatatat atgacatgat gttgttgtta ttcaaaatat taagataaaa atatatagaa
      121 tatatttaaa aattattatg caattagaaa aggagataag tatgcagatt attgatataa
      181 atataaataa tattgacaat gaacatatat gttgttcaat ttctgaaaaa aaaggagaaa
      241 attgtgtttt ttctaaaaaa gcttggatga aaaataattt tgataaaggt ctagtcttta
      301 aacgatttga tgtaagaggt aaagctttta tagaatatat tccagcagaa aatgcatggt
      361 gtcctattac tgcggacaat tatatattta ttaactgttt ttggatatct ggaaaattta
      421 agggtcaagg atattctaat ctattgctac aagagtgtgt tgatgataca aaatcaaaag
      481 gtaaatatgg attagttgtg ctttcgtcta gtaaaaagat gcctttttta tctgatgcta
      541 aatatctaaa atataagggc ttttgtttag cagatacatc agaaccaaat tatgagctat
      601 tatattttcc ttttactgat aatgctccta ttccatattt taagaattgt gtaaaaaaaa
      661 gaaaattaaa tgagaacggt atagttttat attacagtaa tcagtgtcca catacggaaa
      721 aatatgctcc tattttgaaa aaaattgcaa attctagagg tattgatgta aatcttataa
      781 aattcgaaac agcaaaagaa gcacaaaatg ctcctgcacc atttactaca tacagtcttt
      841 tttttaatgg tgaatttgta accaacgaga tactttctga gaaaaagatt gagaaatttt
      901 tagataccaa cattaaaaaa tttaactaaa aagagatgta aatattgtat tttgaaaata
      961 tattatttta taatttaata tattttggat gttttttaaa ttgacgaatg aaatataaaa
     1021 attatatcgc aatttagata caaaatgctg gaaattaacc atatagaaaa tatttccaaa
     1081 aatttaaaaa ttatattgac aaagttaaaa aaataaacta tcatataaaa caataagaaa
     1141 ataaattgaa atcctgtgaa aaagagagta gcataaagct agttgataga gagctgggaa
     1201 tggtggaagc ctagtaacga attttattgt gaatagagct ttggaggaga ttaccagaag
     1261 taatagtatg gtaattggtg aatctcgcct taagagatta gagtgggtaa gattatctta
     1321 tcaattagag tggtaacgcg ggtgtatatt cgtctcttgg tctttgacca agaggctttt
     1381 ttaattttaa atgtacattt tgtagatact ttttaatttg agttttaatg attattgtaa
     1441 ttttaccaat aagagtggta ccgcggaaaa tcgcctctta gtatttataa tgctaagggg
     1501 ttttttgttt taattagagt attttaatct acgtattatc aaatgattat catatagtaa
     1561 agtggaaaga taggatttta agactatttg aatataaaaa tggagggaac aaaatgaata
     1621 aaataaaagt tggtattatt ggtgcaactg gatatgtcgg ggcagagttg attagattat
     1681 taatgaatca tgataaagtc gaagtgacag cgataggttc aaattcgtat gttggaaaag
     1741 atatagttga tatatatcca agtataggat ataaaaataa catgatatgt atagaaaatg
     1801 aaaaagttat agatatgtgt gatgttgtat ttacagccct tccacatgga gttagtgaaa
     1861 agtttgtaat aaaggcaatt aaatcgaaaa agaaagttat agatttaggt gctgacttta
     1921 gaataaaaga tgaagaagta tatagtaaat ggtatggagt atcttttata gataaaatct
     1981 tacataaaaa agctgtatat ggattaagtg aaatatacaa agaagatata aaagatgcag
     2041 atataattgc aaatcctgga tgttatccaa ctagtatttc tttaccattg atgccattac
     2101 taagttctaa attgataaaa aataataata taataattga ttctaaatca ggtcttacag
     2161 gtgctggtag agaattatcg attagtagtc actttacgga ggttaatgaa aatatcactg
     2221 catataaaat agggaaacac agacatactc cagaaataga acaaaattta agtgaatcct
     2281 gcaaagaaaa agtaagtgta gtttttacac caaacttaat cccagtaaat aggggaatct
     2341 tatcaactat atattgtaca aaagaagata atatttctat aaatgatata cataggaaat
     2401 taactgatta ttatgaattt aaagaattta tagaaattct tccattagat aagatagctt
     2461 ctttaaagaa tgttagattc tcaaataaat gtgtaatatc attacatgaa aatggagata
     2521 cattgataat ctgttcagct atagataata tgattaaggg agcagcaggt caagctatac
     2581 aaaacatgaa tataatattt ggtattgaag aaaatacagg tttaaaaaat attgcaccat
     2641 cattttagaa ataaaaaata ctaaactcat ataattagga ggataaatat ggagattata
     2701 aagggtggag ttacagtttc agaaggattt tttgcttcag ggattcactg tggcttgaga
     2761 aaaaataaag aaaaaagaga tttagctttg gtttattcag atgttttatg tgatgcagca
     2821 gcagtagtaa ctcaaaataa agtaaagggc aatcctgttt atgttacaca ggaacacatt
     2881 aaaaatggaa aggcacaagc tatcatagta aatagtgcta atgctaatac ctttaatgga
     2941 aaagaaggac ttataaatgc ttataaaatg gctaaattca ccagtgataa attaaatatt
     3001 aaagaaagtg atgttttggt tgcatctaca ggtgttatag gaaagccatt aaatatagat
     3061 ttaatagaag aaaatatgga tgaattagtg aataatctaa gtaaacaagg tcatattgga
     3121 gcaagagaag ctattatgac aactgatatt ataaaaaaag aaatagcagt agcaataatg
     3181 tatggagata aaaaaataac tattggaggt atggcaaaag gttctggcat gatacatcct
     3241 aatatggcaa caacgttagg atttataact acagatgcaa atattgatgg agttttgcta
     3301 aaagaagctt taaaaattgc tgttgacaaa agttttaata gagttagtgt agatggagat
     3361 acaagtacta atgatatggt aatgattcta gcaaatggaa aagcaaaaaa tgatagaata
     3421 aacaaaaaag atgaacatta tcaagtattt ttgagtgcac ttacatatgt ctgtatagaa
     3481 cttgctaaac ttgttgcaaa agatggagaa ggatgtacaa aactaattga atgctatata
     3541 aatggagcat taagtgaaga acatgcagta aaattagcaa aaacagttat atcttcttca
     3601 ttagtaaaga cagctgtatt tggagctgac gcaaattggg gtagaatact gtgtgcttta
     3661 ggttatgcag gtgaagaaat agatatggaa aaagttgata ttatttttga gagtatgaaa
     3721 ggatatattg aagtatgtaa aaatggaaat ggattagatt ttaatgagga aaaagcaaaa
     3781 aaaatccttg aagatgacga aataagtata ttagtagatt taaatatggg caatgccaga
     3841 ggaaatgcat ggggatgtga tttaagctat gattatgtaa gaataaatgg aagttataga
     3901 aattaggagg atatatttta atgattaata tagaaaaagc aaatacactt atagaagctt
     3961 taccatatat agaaaaacat cagggaaaga ctattgtagt taagtatggt ggaagtgcaa
     4021 tgaaaaaaga tggtctaaaa gaatctgtta tggaagacct tgtgttaatg agttatgttg
     4081 gaatcaatat agttttagta catggtggag gggcagaaat caataagatg ctagcaaaag
     4141 tggatataga aagtaaattt gtaaatggtc ttagatatac agatgaagag accatggaaa
     4201 ttgtaaaaat ggtacttgct ggaaaggtta ataaagacct agtaaataaa atacatacaa
     4261 aaggtggaaa agcagtaggg ctttgtggaa tagataataa tatgatttta tgtgacccat
     4321 ataaaaatta tgaacttgga tttgtaggag aaatcaaaaa agtaaatgtt gagcttatag
     4381 aatcttgtct aaagtcaggt tatatttcag taattgcaac tataggtgtt ggagatgatg
     4441 gagagactta taatataaat ggtgacacag cagcttctgc tatagctaaa gagttaaatg
     4501 cagataaatt gatacttctt acagatgtcc ctggtttgtt aagagagcca gatgaagaaa
     4561 aatcattaat aacagaagtt atattagagg atgttgataa attatttgaa gaaggtataa
     4621 ttactggagg tatgatacct aaaatagagg gatgcgtaga tgccttaaat aacggagtaa
     4681 atagggtaca tattttggat ggaagagttc cacattctat aataacagag ctatttactg
     4741 atagtggaat aggtacatta ataagaaaag aaaatgaata aaattataag tttaagaggg
     4801 gagaataaga atgaataacg acaatacaat ttcaaaatgg aatgaatatt ttatagatac
     4861 atataaccaa cccaattttg tcatagatta tggtgaagga agttgttttt ttgatactaa
     4921 tggcaataaa tacatagatt ttacaagtgg atatggagta tcttctcttg ggtatagtaa
     4981 tagtaattta aagaatgctc taaaagagca agtagataaa ttattacata cttcaaattt
     5041 atattttaat gagccagtgt tgtattcagg agagaaaata attaattcaa gtggaatggc
     5101 taaagtatat ttttgtaatt ctggaacaga agcaaatgaa acagcattca aaatagcaag
     5161 aaagtatagt agtgacaaat atggtaatgg tagaggaact atcataagtt taaaagactc
     5221 ttttcatggt agaacaatga tgtctttaat ggcaactggt atggacaaat accataagta
     5281 tttttatcct cttcccgaag gttttaaata tatagaaaga aataatattg aagatttaaa
     5341 aaataatctt gattccacag tttgtgcaat aattcttgaa gcgattcaag gcgaaggtgg
     5401 agttaatgtt cttgaaaaag attatgtttt ggaaatagta aaaatatgtc aagaaaagga
     5461 tattgtagtg atttttgatg aagtccaatg cggtatagga agaactggta aactttttgg
     5521 ctatgaatat tttgatgtaa aaccagatat agttaccgta gcaaaagggt tgggagcagg
     5581 aataccagta ggaggagtat tggtaaataa aaaactttca aaagtactag gaaaaggaga
     5641 ccaaggtaca acttttggag gtaatttatt agctatggtg gcagcttcag ttgtattaga
     5701 tgagatatct aaagatggtt tttataatga agtattagaa aaaggaaatt atataagaaa
     5761 atctattgaa agttttaata acaaagttgt attaaagaca aaaggtattg gtctaatgat
     5821 aggaattgaa actaatattg aatctagtat tatagaagag aaagctagaa aaaaaggatt
     5881 gcttattttg actgctggaa aaaatgtatt gagattttta ccaccattaa caatcagtta
     5941 taaggaaata gatgaggctc ttgaaatcct aaaagatata ttactagaaa ttaactaaat
     6001 attcaaaaaa tagttgatta aagataaaaa ttagaagtaa ataaaaaagt aaagtgctaa
     6061 caagtttaat tgaatttaaa aagtttataa gaaggggcga attaatatga ataatataag
     6121 tttaaaaggt aagagttttt tgacattaaa ggattttaca aaagaagaga ttagatattt
     6181 attaacttta tcaagttatt taaaaactaa gaaaaaagtt ggtataaaag gcgatttgct
     6241 acaaggaaaa aatatagctt tattatttga gaaaacatca acaagaacta gatgttcatt
     6301 tgaagtagca gctcatgatg aaggagcaca tgtaacttat ctaggtccaa atgattgtca
     6361 tatgggtaaa aaagagtctg tatcagatac agcaaaagtt ctaggaagat tctatgatgg
     6421 aatagaattt agaggcttta aacaagaaac agttgaaagt ctagcaaaat attctggtgt
     6481 acctgtttgg aatggactta cagatgaata tcatccaaca caaatactgg cagatttttt
     6541 aacagtaatt gaaaatgtag ataaagattt gaataaagtt aaatttgtat atgttggtga
     6601 tgctagaaat aatatgggaa actcactgat gataggatgt gctaaaatgg gaatacattt
     6661 cgtagcacta gcacctaagg aactttggcc aaatgaagaa ttggttaagg aaatgaagga
     6721 aatagccaag aaatccaaag gagatataat gttgactgaa aatattgatg atgttaaagg
     6781 tgcagatgtt atatatacag atgtatgggt ttctatggga gaagaggagc aatatgaagc
     6841 aagaataaat caacttaaag attatcaagt aaatatggat atgataaaga aaactgaaaa
     6901 tgaaaatgtt atatttttac actgtcttcc agcatttcat gatttagaaa ctaaggttgc
     6961 tcaggagatt tatgaaaagt tcggattatc agaattagag gttacaaacg aggtatttaa
     7021 ttcaaaatat tcaaaggtat ttgaagaagc tgaaaataga atgcatacaa taaaagctgt
     7081 tatggtttct actatgactg atgtaaatat gaacaataat ttagtataga tatgttttaa
     7141 tatagacttt actttaatga aaatttataa aatattttaa atatattatt caaaaactct
     7201 cattatatgt taaaataact tatgtatcaa caaattaaga gggaggattt gagatatgaa
     7261 aaaaatcata ttagctttaa caactttttt attagcaata tctttaacag catgttcaag
     7321 tgcaaaacca gaagatacaa ttgatagttt ttttaacagt gcgaaaaagt ttgactttga
     7381 aggcatgaat aaggtaatgg aaaataatga cgaaaaatat aaggatatat taaaggaatt
     7441 agatacaaaa gacccaaatg cccaatatgt tttagattat ctaaaacaga atgcatctaa
     7501 aatcacatat acaataaaag acagcgaggt taaaggtgat aaggctacaa tcaaagtaga
     7561 atgtaaattt atagattcaa caccattact tcaagaaata gtagctgagg catttacaaa
     7621 gatgattggt atgagttttt caggacaaga cttaactgat gagaaaacaa ctgaaatgtt
     7681 agtatcaatt atgaaagaaa aacaaaaaag cgttaaagag acttatgtta caaaaaatgt
     7741 agagtttgaa tgttctaaaa aggataataa atggataata agttcagtaa atgatgcagt
     7801 agctgatgta ttgttatcta atcttgttac agcaggacaa gaattttcta actctatgaa
     7861 tacacttgaa taatattgtt taggtgtagt aaagtaataa atatatgatt atttttataa
     7921 ataaacatat aaatatttat gaaggatacc atttttggta tcctttttat taatataatg
     7981 aaatacatgg ttaatttcta tattgtcaag aaactatgat atatgttata agtctatact
     8041 atttaaaata aacttataac atatattttt tataatatag tgcatgaaaa atttatttaa
     8101 ctaattttag atatttattg acaactgatt cagctaagca ttttgatgaa tcaacaatat
     8161 ttattgaagg atatgatttt tctaaaacta catttaaatc agtacaagca attactatat
     8221 tttctataga ttcattttta acatcttcga taagtttatt ccaaatatca atattgtgtg
     8281 gattttcttt atccattttt atattttgaa taagattgtt taattttatt tgccattcat
     8341 ctttaaatat aaattcatgt ccattatgta ttataccatt ctggtaaatt gtagactcaa
     8401 aagttgaact agtcgaaaaa agagttactt tttgtgagat agtaggtaat tttttaactg
     8461 tttcttctac aatatttaat attggtatat taatagactc ttttaattct ttaaagtata
     8521 tatgtgctga attgcatggc attgcaataa aatttacacc agttgattca agcttttgta
     8581 atccatcaat tatagtttct ttcataagtt catgatttat tggacggtca atataaaatg
     8641 gggtaggtaa tgaatatatc atcatctttg gaaattcatc atcatatttt gctccatatt
     8701 gagattgaca ctcatcaata attaaatcaa caaaaggggc tgttgacctt ggacccattc
     8761 cagccaaaat accaattaat ttttcattca taaaaattat ctcctttata tataaatata
     8821 tttatgtata aaataaaaat ctataaaagc ataaaattat attttataat agattatatt
     8881 gtacacttat ataatataaa tataaagtga ttttttagat tatatatttt aatatacttt
     8941 cttatataag aattatttta aatcaagatt atctagtatt tttaaaaata tttttacagc
     9001 agtatccaag tcatctacag aaacactttc attaggatta tgacttacgc ccccagcgca
     9061 tcttataaat accataccaa tatcagttat attatccatt tcctgagcat catggccagc
     9121 accactataa atataaaaag gattaagatt taaatctata aaactttttt ctatgatttt
     9181 tgttatttta ttagaacatg gtacaggaac attttcaaaa gcaagttcac ttgtataatt
     9241 aagttttcta ttttcacata tatgtgatat ttcattaaat atcttttcta cagaattatc
     9301 aagaatttct tgtgaacaag agcgtacatc aagagtaaat tcgattattc tatataaaat
     9361 ggctaaatag ctggatttaa gtaaaaaaaa tagaatttat cattattttg ccacccattt
     9421 gccaccgtaa tttttttcgg tggcaaatta tctaacaaga ttttctaaaa tatctattgt
     9481 ttcactttcc attttacttg taacatgtga atatgtgtcc atagtagttg ataattgact
     9541 atgtcctaat ctttgttgta tatattttat attagctcca ttttctaata ataatgtagc
     9601 atgtgtatgt cttaaacaat ggaagttgaa gtcaaggttt aattttttgt taattgttct
     9661 tatacaagca tcaatgttgt tatgatttac aaatgagcca tctttttttc tacaaaccca
     9721 atcatactca gtttctgtat accattttcc aagtttaatt ttttgctttt tttggtttaa
     9781 tttttcttct tttaatactt tagataaagt atcacctatt ctaatatctc ttatggatgt
     9841 ttttgtttta ggtgaagtta attcaaactc tgaaactttt ctctttatta aattttttct
     9901 aactttgatt atgttattat ctaaatcgat attgtcccaa caaagaccta atatctcccc
     9961 ttttctcatt cctgtatgaa atcctataag caaaggaata tatatatttg tatttttagg
    10021 atatatctct agaattttat taaattcatc caatgttata gttttgtttt ctgaatcttt
    10081 ttttgtatct ataatatttt ttggtatgtt ggtgtattga gcaggatttt ctttaataag
    10141 cttgtaagga taaacagcag attttaaagc agagtttaat acaccataga ttgctttcaa
    10201 attacctctt gtatagcgtc tttcttctcc attttgagta tatgtttctt ttgttttatt
    10261 gttcaggaa
//
LOCUS       DAELJO010000141        10252 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELJO010000141 DAELJO010000000
VERSION     DAELJO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.141"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            45..800
                     /locus_tag="KPB88_002806"
     CDS             45..800
                     /locus_tag="KPB88_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055071.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            897..1934
                     /locus_tag="KPB88_002807"
     CDS             897..1934
                     /locus_tag="KPB88_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055072.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            2744..3646
                     /locus_tag="KPB88_002808"
     CDS             2744..3646
                     /locus_tag="KPB88_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055073.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            3633..4388
                     /locus_tag="KPB88_002809"
     CDS             3633..4388
                     /locus_tag="KPB88_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055074.1"
                     /translation="MLQIRNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            4519..5541
                     /locus_tag="KPB88_002810"
     CDS             4519..5541
                     /locus_tag="KPB88_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055075.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(5705..6262)
                     /locus_tag="KPB88_002811"
     CDS             complement(5705..6262)
                     /locus_tag="KPB88_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0055076.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(6268..7344)
                     /locus_tag="KPB88_002812"
     CDS             complement(6268..7344)
                     /locus_tag="KPB88_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG0055077.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(7345..8028)
                     /gene="modB"
                     /locus_tag="KPB88_002813"
     CDS             complement(7345..8028)
                     /gene="modB"
                     /locus_tag="KPB88_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG0055078.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(8177..8989)
                     /gene="modA"
                     /locus_tag="KPB88_002814"
     CDS             complement(8177..8989)
                     /gene="modA"
                     /locus_tag="KPB88_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055079.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKNKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            9182..10111
                     /locus_tag="KPB88_002815"
     CDS             9182..10111
                     /locus_tag="KPB88_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055080.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         3649 a         1559 c         1425 g         3619 t
ORIGIN      
        1 caaagctagt accaaaaata aatcagaaaa aagaggtgtt gtagatgtta caaattaaaa
       61 atctttcaaa gagtttccct aatccatatg gtgaacctaa tactatcttt gaaaatttat
      121 caatcgatat agaagatggt gaatttgtaa gtatcatagg tagtaatgga actggtaaat
      181 ctactttatt aaatataata tctgggctta taaaagaatc ttctggtcaa attactttag
      241 atagtactac tataacaaat ttagctgagt ataaaagaac tcaaatagta agtagagttt
      301 ttcaagaccc aagccttgga acttgtcctt ctatgacggt tagagaaaat ttatctctag
      361 cacttaataa agggaaatta ttgaacttaa aaaaatgtct tcgtcataaa acgaatgact
      421 tggaacactt acttgaagga atttctttgg atttaaaaaa atatttagat attaaagtac
      481 aatatctttc aggaggtcaa agacaatcac tatcactaat tatgtctagc ttagcaagtc
      541 ctaaggtctt attactagac gaacatacag cagcacttga cccaaaaaca tcaaatgaag
      601 taatagagtt aacagataaa atcgtaagag agaaaaatat aactacactt atggttactc
      661 acaacttgaa acacgctctt caatatggag ataggttgat aatgcttcat aagggtgaag
      721 ttgtattaga tgtaaaagat aaagaaaaag aagatttaac agttgaagaa atcttagaaa
      781 aatttgaata tgcagtataa gaaattaaaa tcttagtttt aatcagaaat atttataatc
      841 tatcatatga atatatgaac aaatataatt aataaatttt aagggaggaa aataaaatga
      901 taaaaagtaa aaaaatatta agtttaatta tagcaggagt acttggtgta tcaatgctta
      961 ctggatgttc tcaaaatgat ggctccaatg cttcaaatga aaataaagaa acagatagca
     1021 aaaaacaaaa aaatataggt attagtcaat tagtggaaca tccgtctctg gataaagcaa
     1081 agaaaggatt catcaaagca ctcgaagata aaggctataa agatggagat aatataaaaa
     1141 tagatttcca aaatgcacaa aatgatatgc ctactacaca aagtattgct agtaagtttg
     1201 tatccgacaa aaaagatttg atatatgcta tatctactcc atctgcacaa gcagcttata
     1261 atgctactaa agatattccg ataataatga ctgctgttac agaccctgta gaagctggac
     1321 ttgttaaatc tcttgaaaaa ccaggtggaa atgtttctgg tacatctgat tatctttcaa
     1381 ttgataaaac attagaattg gttaaaacat tgactccaaa agcaaagaaa ataggggtta
     1441 tatacaatac tagtgaagtt aattcaaaaa tccaagttga ttctctacat gattatgcta
     1501 agaaaaataa ttatgaagta gttgaaaaag gaatcagctc ttcaagtgaa gttaaccaag
     1561 ctatttctag tttagttggc aaaatagatg ttttatatgt ccctactgac aatttaatag
     1621 tttcttctat gccaatagtt tctaaagttg ctaatgaaaa caagattcct ataatagcct
     1681 ctgaagaagg ttctgtatct tctggtgctt tagcttgttg tggaatagat tatgaaaaac
     1741 taggttataa ggctggagaa cttgctattg aagtattaga aggaaaatct gttggcgata
     1801 taccagttac tacattagat gaaactgaaa taataattaa cgaagataca ctaaaagcac
     1861 ttgacatgca aaagttatca gcagataata taaagtatat aaagtcagat gaaaatgcaa
     1921 aatctgcaaa ataaaactta ttaatttcaa aaaataggta atatgaagta ggtaatatga
     1981 aaactttata taataagtac tcttatccac cctaaccctt tgaaaattgt gcttattgta
     2041 gcaatttgaa tattgcatat aaagttattc attttccata aaagtcgtct atattgacga
     2101 ctgattatga aagttatata actttctaat cactacttga gctgtccaaa agaattttaa
     2161 tattctccag acagcttttt tttatgatat ttgattgata tacatgtcta gttattccat
     2221 atttagtaaa tattttgata aaatttccac attcacttat aacttaggat aacgtttttt
     2281 tgcaaacaat acattaattt aaaagtattc tgagtataca ttatttagta ttttaattaa
     2341 atttagcagt tgacttttat tattgtataa tttataattt ataacaagtc gagaatacat
     2401 ataattttct gactaattaa aatattaaaa gggagagata attatgaata ctttattaaa
     2461 caatagaaaa tattattatt attatagcca ctatttttag ggtttgtacc tacgttttaa
     2521 catagataca gaccccttaa gactactaaa attttgggat tgtatctatg ttggctatat
     2581 tgtttataaa gttttcattt aactatataa gagccacata gattaaacta tgtggcttat
     2641 tcgtgttttt accacatagt tttgtacact atgtggtttt tttatacaca aaattagcaa
     2701 ataattaaaa ttataaaaat attttcaaag gagtgtttta tttatgtcag gtattatatc
     2761 tgtaatgacg caaagtttaa ttttatcgat tatggcactg ggggtttata taacttacaa
     2821 aatcttagat tttccagata tgtctgctga tggtagctac acaatgggtg cttctattgt
     2881 agcattttct cttactaatg gtatttctcc tgtagtagca actttaatgg ctatcttatg
     2941 tggttgtact gctggtcttg ttacagggat tcttcacatt aagtttaaga tatctaactt
     3001 actttctggt attttagtta tgggaatgct atactctatc aatttaagaa taatgggaaa
     3061 atcaaatatt cctctattta gctttaaaca cttattcaat ggggaaatat ctcctatagt
     3121 tttagcctta gcttttgtat tcatctgtaa agtcctttta gatttattcc taaaaacagg
     3181 tcttggatac actttaaaag gtgttggtga taactctcaa atgattaaat ctctaggtat
     3241 aaatataggt tctataaaaa tactaggact tatgatttca aatggactta tagctttatc
     3301 tggtagtctt atggctcagt tcttgggatt ctcagatgta aatatgggta taggaacttt
     3361 agttcttgga attgcctcta tcataattgg tattacacta tttaagaaat ttacttttat
     3421 caaagatact actgcaataa ttgttggctc ttttatatac caattcacta tatactttgc
     3481 aatgagttta ggaatgttat caactgactt aaaactaata actgccatag ttataatagc
     3541 tttcctagct actggtaact taaatatttc actaaaaaaa aacaaatgca aagctagtac
     3601 caaaaataaa tcagaaaaaa gaggtgttgt agatgttaca aattagaaat ctttcaaaga
     3661 gtttccctaa tccatatggt gaacctaata ctatctttga aaatttatca atcgatatag
     3721 aagatggtga atttgtaagt atcataggta gtaatggaac tggtaaatct actttattaa
     3781 atataatatc cgggcttata aaagaatctt ctggtcaagt attattaaat aaacataact
     3841 tgtctaattt agctgaacac agaagaactc aaatagtaag tagagttttt caagacccaa
     3901 gtcttggaac ttgcccttct atgactgtta gagaaaactt gtctttagct cttaacaaag
     3961 gaaaactatt aaatctaaga aaatgtcttc gttacaaaag agatttctta gaaaatttat
     4021 tggaaggtgt atctcttgat ttaaaaaaat accttgatgt tcaagtaaaa tttttatcag
     4081 gaggtcaaag acaatcatta tcactaatta tgtcgtgctt aacaaatcca tcagttttat
     4141 tacttgatga acatacagca gcacttgacc caaaaacatc aaatgaagta attgaattga
     4201 ctaataagat tgtaagggaa aaaaatataa ctacacttat ggttactcac aacttaaaac
     4261 acgctcttca ttatggtgat agattgataa tgcttcataa gggtgaagtt gtattagatg
     4321 taaaaggtaa agaaaaagaa caattaacag ttgaagaaat cttagaaaaa tttgaatatg
     4381 cagtttaaaa taatatatct attgtattaa actcggcttt aaaataaagt taaaattaca
     4441 ataattatgg ttactttgta ataattcata tgaatatatg aacatatata ataataaatt
     4501 tttgaggggg atataaaaat gattaataaa aaaagattgg caagtttgat tttagctggt
     4561 gctcttagta tatctatgct tacagggtgt tcacaaggag gagactctgg taattcaaaa
     4621 caagaaagta attctaaagg taaagaagtt aaaaaaattg gtataactca actggttgaa
     4681 catccagcat tagatgccac taggacaggt tttgtaaaag cattagaaaa gaacggattt
     4741 aaagatggag aaaatattga tatagacttc caaaatgctc aaaatgacat gcctactaca
     4801 caaagtattg cgagtaaatt tgcatctgat aagaaggact taatatttgc tatatctact
     4861 ccatcagctc aggcagcatt taatgctact aaagatatac ctatacttat tactgctgtg
     4921 agtgaccctg ttgctgctgg attagttaaa actcttgaaa agccaggcac aaatgtatct
     4981 ggaacatcag attttgtttc tgtagacaaa ggtttagaac ttcttaaaat atttgctcct
     5041 aaggcaaaaa ctataggagt tatgtacaat actagtgaag taaattccaa agttcaagtt
     5101 gatgctttaa aagaatatgc atctaaaaat ggatttaaag ttgttgaaaa aggtataact
     5161 acttcaaatg aagttaacca aggtatttct agtttagttg gcaaaataga cgtattatat
     5221 gttcctacag ataacttagt ggcttcttct atgcctatag tctctaaaat agctactgag
     5281 aataaaatac ctgttatagc agctgaatct gggccagttg aaaaaggtgc tcttgcttgt
     5341 caaggtatca actacgaaaa actaggttac aaaacaggtg aaatggcagt taaaatttta
     5401 aatggcgaat cagtatctga tatgcctgtg gctacatcag atgatacaga tatcattgtt
     5461 aatgaagata tattaaaagc tttaggtatg gaaaaacctt ctaatgaaaa tatttcttat
     5521 gtaaagacta aacaagaata gaaagatatt ttctatatac gattaatatg cctgtttcta
     5581 taaattaatt tatagaaaca ggcatatatt tttattttaa tgtattatct atttaaagat
     5641 tatatgtaaa tgctcttaat ttgtttgtat aaattttact tgtataaact ttatacaatc
     5701 atatttattc ttctattttc tttattactc tacatggatt tccaacagct acagtatttg
     5761 gtggtatatc ttttgtgact acacttcctg cacctattac tacattatca cctatagtaa
     5821 ttcctggtgt tatgattact cccccaccta tccatacatt atcacctatt tttataggag
     5881 aaccatactc tatgcctgaa tttctaagtt gtgcatcaat tggatgatag gctgtataga
     5941 tttgaacgtt tggagctaac attacattgt cacctatttc aattttacat acatctaaaa
     6001 aaatacaatc ataatttgca aagaaatttt cgcctacatg tatgttatat ccataatcac
     6061 acctaatatt ttgttctaca tttatttgct tgccaactga accaaatagt tgtcttaaaa
     6121 tatcttctct tttttcatat tcatcctcta aagtattatt aaacaatcta gttaattttt
     6181 tacaatactc tctttctttt actaacaatt catcatttgc atagtatcct ttcccagaca
     6241 acattttttc tttttcagtc atatagttta ctcctttttg ttataattgt atctaaagca
     6301 aaataaatat tcctgtggaa aatctaaata tattctgtca cctttcttaa atctcatttt
     6361 acttttttcc aattctattt gaataggtac acattcgtta gataaatcag tttttctaac
     6421 atatatagta tatgtaaaag gattttctac aatgttaatc aattcgaaac ataacttttc
     6481 atttaaatct tcattagaat ccaagacctt aatatgatgc tccctgattc ccacatattc
     6541 tatattatca cctatttctc tatttaatgt caactcgcat ccccagtctg tagcataaat
     6601 agtattttta tccagtctat ttaatttgga tatattttta caccctgtta tcttagcttc
     6661 tataaggtac tttggagatt caaagagctc ttttttaggt ctctttggaa gaccttctcc
     6721 tttgttgtac actataatat catcacaaac tcggtaagct tcttcaatat catgagtaac
     6781 aaatagtata tctcccttgt aatcttttaa catattcata agttcttttt ccatattgct
     6841 tcttaaatga tggtccaatg ctgagaaagg ttcatctaaa agtaatatat ctggagatgt
     6901 tgccaatgct ctagctagtg ctaccctttg ttgttgacca ccagataatt gccaagggta
     6961 tctcccctca aacccattta atttaagtat atctaagtac cttgctacta tttccttttt
     7021 ttcactctta cttagtttta aaagcccaag ttctatattt tgacttacag tcatatgtgg
     7081 aaacaaagca tagttttgaa ataaaaatcc tacctttcta tcttgtgttg ataaatttat
     7141 cttcttttca gaatcaaata gaactctatc atttaataca attttacctc ttgttggttt
     7201 ctcaagccct gctatacatt ttaatgtcat actttttcca gaaccagatt ctcccaaaaa
     7261 ccccagagta cctttttctt gtttaatttc tacctttagt ttaaaagagg ataaatcttt
     7321 ttcaatatcc acgtataagc tcaactatcc acacctcttt cctataattt tctgctgaaa
     7381 ttccgaccaa taatttagta ataaaatcat tgctatagat attgctacaa taatcataac
     7441 ccacatcata gctttattca tatctccgcc ttcaacagca aaaaatatag ctattggcat
     7501 agtttgagtt tttccaggta tatttccagc tatcataagt gtcgcaccaa attcgccaag
     7561 agctctggca aacgacagca ccaatccacc aattattcca ggccatgcta atggaactgc
     7621 aatcttaaaa aatactttcc attcattaag acctaatgtt ctagcagctg acaacatatt
     7681 attatcaatc tgttcaaaag ctgaccttga agttctatac atcataggaa aagacaccac
     7741 tactgctgaa attacagttg ctgtccaact aaatattaaa gtcctatcga aatttaataa
     7801 aaactttcct ataaatccat tcttcccaca tagtaataat aaaaagaaac ctacaactgt
     7861 tggtggcaaa attagtggaa gtgtaaatag tccatctata agacccttcc acttacctct
     7921 atagttagcc ataatatatg acacaaatat tcctattaca aaagttatta ttgttgacaa
     7981 aatagatgtc ttaagtgata tccataaggg tgaccaatct gttcccatct ttccctcctt
     8041 aattttaaat ggaaatagtg aaactaataa ctctattaat ttcactattt ccattttatt
     8101 ataatacaca attattttca attttcattt atttatatat aatttatact taattacaca
     8161 taatttattt ctaagtttat tctacttttt tatatccaaa ttcttcaaaa atcttttgtc
     8221 ctgactcact taataaaaat tcttcaaatt tcttagcagc atctacgttt ttactagctt
     8281 taattactga aactggatat gtaataggcg aatgagtttt ttcgtctgtt ttttcaacta
     8341 cttttatctt atcattattg acagtatcac taaagtaaac gaatccaaca tcagcatttc
     8401 ctgattgcac ccatgctaaa acttctttta catcttttgc aaatactagc ttatctttta
     8461 acttattttt taaatttaaa ttagttaaca cctcatctgc atacttacca gctggaacac
     8521 tttcagcttc accaacagca attttcttca ctttatctgt agtcaaatct ttcatacctg
     8581 atacactact gtcagcagaa gaaataagaa ctaaatcatt ttttactaaa tctttcatag
     8641 tatcagaaac taataacttc tcttcatcca aagctttcat ttgtttttga cctgctgaaa
     8701 taaataaatc acaaggtgct ccttgttcta tttgttgttg taatgagcct gatgctccat
     8761 agttaactgt aagttttaca tttgaatcaa cttttttata ttcttcttca atctttgcca
     8821 ttgcttcttt taaacttgca gcagccgata tatttaactc tactgaatca ctacttttag
     8881 ttgttttttc ttgttttcca tcatcttttt tgtcttcatt actattgcac ccaactgtcc
     8941 ccaaagttaa acatgcaact aatcctaata ttcccaatat ttttttcata acaatctccc
     9001 tttttgtaaa tttgatacac attttaacat gttttattat gttttatcat gttttgttat
     9061 atatagttta attatatata catgataata aattttcaag taatttattt acactacttt
     9121 taaatatttt gctacatttt ccttttttta atgttaaaat aaccttatat ggaggtggat
     9181 tatggactca ttatcatctt tgacacctgt cgaggtagct gaattattaa agataacaaa
     9241 aaatactgta tatgaattaa tcaaaagagg tgaacttcct tcttataaag tagggaaaaa
     9301 aataagagtt gatactaaag atgttgaaga atatatcaac agccaaaaaa ctggtaaagt
     9361 aaaggataca catttcaaca ataaaaatat agatataaat aataatttca aaattattat
     9421 ttctggtcaa gatgtaatat tagacatttt agctaggtct gttgaaaaaa aattggatgg
     9481 aataagtaca tttcgctctt atataggtag ttataatgct ttgtatgagt tatataatgg
     9541 cagagtttct atagcatcat cccatctatg ggatttcgaa acggatgaat ataatagcac
     9601 ttttgttaaa aagctattgc caggaatacc atgtgtactt ataaatctag cttatagaat
     9661 gcaaggtttc tatgttgcta aaggaaatcc taaagagata acaacctggg aggatttaac
     9721 aaaaccaagt ataaccattg taaataggga aaaaggttct ggaactaggg ttttactaga
     9781 tggtaaactt agacttctaa actttaatgg taaacatata aaaggttatg ataatgaaga
     9841 attatcacat cttggagttg caagtactgt ttctcgtgga atagcagatg ttggtttggg
     9901 aaatgaaaaa gctgctttac aagtaaataa tatcgacttt attcctcttc acaaagagcg
     9961 ctatgattta gttattaaaa aagaagactt acaaaatcca gtttaccaaa ctataattaa
    10021 cataataaac tctcctgagt ttaaagctga acttcaaggt cttggcggat atgatttaac
    10081 tgatactgga aaaactatag caaaagttta agattaaaat cgcaatctat tttataagat
    10141 ttataatttt tcacaaaatt tataacttaa acacgaaata aattgcgatt ttagtgtctc
    10201 aaaaaattta tgtctttcat tttatcatcc aatacaaaat ttaatataat ta
//
LOCUS       DAELJO010000142        10177 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELJO010000142 DAELJO010000000
VERSION     DAELJO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.142"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            84..251
                     /locus_tag="KPB88_002816"
     CDS             84..251
                     /locus_tag="KPB88_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0055081.1"
                     /translation="MTNERVRFTFRLPAPLLKKIKNRASIEGSSMNSLILHILWDYIQ
                     EVESEEAKKHE"
     gene            244..1023
                     /locus_tag="KPB88_002817"
     CDS             244..1023
                     /locus_tag="KPB88_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG0055082.1"
                     /translation="MNNLSVIKNEGLIKVEVNEKQEQILSARDLHEFLEVGSRYNDWF
                     NRMIGYGFVENEDFISITQKKVTAQGNETEYIDHVIKLDMAKEIAMIQRNEKGKQARK
                     YFLQIEKDWNSPEKVMARALIVANKTIEKKSREIEEKDKVIQLQQPKVLFADSVASSN
                     DSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVNLGVIETK
                     EGTRVHPNGYISVTKTPKITGKGQVYFINKFKSSKQISMLS"
     gene            1059..1313
                     /locus_tag="KPB88_002818"
     CDS             1059..1313
                     /locus_tag="KPB88_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055083.1"
                     /translation="MSEDIFYGIKNTLDEIAEVQIKNKQGVLKEVGMLISGEDNSCIT
                     HCLDEDLIKFYIKEEAVLTIDKDSHLLCMLDALFYNFLDK"
     gene            1341..1784
                     /locus_tag="KPB88_002819"
     CDS             1341..1784
                     /locus_tag="KPB88_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055084.1"
                     /translation="MKKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKELREGVI"
     gene            1912..2100
                     /locus_tag="KPB88_002820"
     CDS             1912..2100
                     /locus_tag="KPB88_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055085.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQRGSKKFNKEKKVKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            complement(2235..2405)
                     /locus_tag="KPB88_002821"
     CDS             complement(2235..2405)
                     /locus_tag="KPB88_002821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055086.1"
                     /translation="MSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSIGREIEF
                     ILEKYFEDNPLE"
     gene            2548..2706
                     /locus_tag="KPB88_002822"
     CDS             2548..2706
                     /locus_tag="KPB88_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0055087.1"
                     /translation="MTNERVRFTFRLPAPLLEKIKNRASIEGSSMNSLILHILWDYIK
                     EIENKEVK"
     gene            2706..2933
                     /locus_tag="KPB88_002823"
     CDS             2706..2933
                     /locus_tag="KPB88_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055088.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            3056..4018
                     /locus_tag="KPB88_002824"
     CDS             3056..4018
                     /locus_tag="KPB88_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBG0055089.1"
                     /translation="MNKISSRRKYLDAFIVTDTKNIDKIDWLKNRQLGIGGSDASAVA
                     GLNPWKTSVQVYIEKKEEIPIETKSFRMELGNRLEGLVAELFTEETGLKVRNVNGMLK
                     NEKYPFAIADIDRAIVGEKAFLECKTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATH
                     CYIAALLGNEKFVWHKINRDNEVIKNLMKIESEFWEENVLKDILPIPDGSDAYSEFLK
                     TRYKNSVKEKIELNLLEDGISKLKRYDDIVLQMKELKGEKQLIEQEIQSEMREFELAT
                     LGGRIITWKGATKRSIDTKRLREEMPDIAEKYTNISSYRTFKIK"
     gene            4031..4864
                     /locus_tag="KPB88_002825"
     CDS             4031..4864
                     /locus_tag="KPB88_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBG0055090.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGHSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEEAINV
                     D"
     gene            4879..5817
                     /locus_tag="KPB88_002826"
     CDS             4879..5817
                     /locus_tag="KPB88_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055091.1"
                     /translation="MNEDKSVIEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRVHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISKRGLKEEKKKKKPCTKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIVKQPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNHV
                     SKNKFANFEQTFTKYSENELDDIIKKSQKEKFGVGS"
     gene            5837..6235
                     /locus_tag="KPB88_002827"
     CDS             5837..6235
                     /locus_tag="KPB88_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055092.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCIRKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEV"
     gene            6238..6510
                     /locus_tag="KPB88_002828"
     CDS             6238..6510
                     /locus_tag="KPB88_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055093.1"
                     /translation="MKHECKKLFLECDKGSFEINDTRIGEVMFESTEIDNPFERVKYE
                     GKATFEIVSGWEYLQREMLWLKILHLSAVVAKIMQYKMLGILIREM"
     gene            6515..6895
                     /locus_tag="KPB88_002829"
     CDS             6515..6895
                     /locus_tag="KPB88_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055094.1"
                     /translation="MAKIWMDAGTFLEKTIDIEDMFELNLRAIRKRNEKIKNEIEKQI
                     KYEDSEIKTTNRGGPGKIIKIFNINTGEVKILKSAEEASRYIKVSRSYASYLARENKS
                     TEDGWKAEYIQGATDGISKCGTSN"
     gene            6864..7004
                     /locus_tag="KPB88_002830"
     CDS             6864..7004
                     /locus_tag="KPB88_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055095.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSRYECEGML
                     EK"
     gene            7120..7287
                     /locus_tag="KPB88_002831"
     CDS             7120..7287
                     /locus_tag="KPB88_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055096.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAEQGRVRILDLETKEKY
                     DLLKNKLEEML"
     gene            7406..7645
                     /locus_tag="KPB88_002832"
     CDS             7406..7645
                     /locus_tag="KPB88_002832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055097.1"
                     /translation="MKKYKIKFEEKVTLEHEVIVEIPNEININDICNCIENKCQRVYD
                     IADYIREFNGRQIDFTEDTCGETEMLVESFRKYKE"
     gene            7760..8563
                     /locus_tag="KPB88_002833"
     CDS             7760..8563
                     /locus_tag="KPB88_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBG0055098.1"
                     /translation="MNKVLNWPGAKWSMSKNIVGILPKHNIYLEPYFGSGAVFFNKKA
                     CNTEILNDADKQIVNLFKCIRDNPNELMNAIYFTPYARDEYMNCNILETDNDIEKARK
                     FIIRTNMARGGIQNYKTGWKHTGPKESKKCFQKVVGKWNKLPKIILDTAIRLKDAEIE
                     NRDAVELIKKYNKTDCLIYADPPYLLKTRSQKMYNVEMETEEEHEKLLKTLLKFSGYV
                     ILSGYDSDLYNEMLNGWNKMEFKACAEQGKRKEMLWTNFETTKQLSLFK"
     gene            8576..8983
                     /locus_tag="KPB88_002834"
     CDS             8576..8983
                     /locus_tag="KPB88_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0055099.1"
                     /translation="MRINFTIDGEPVGKERPRFNLATKRTYTPNKTKSYEELIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            9296..10132
                     /locus_tag="KPB88_002835"
     CDS             9296..10132
                     /locus_tag="KPB88_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG0055100.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHDIEGGFGEDKKSMSVKDIAKI
                     HNRELKHVNELINNNIKRFKDGIDILDIKVSRSERPSYLKSLSLEMGYSNQSYANANN
                     IYLLSERGYSKLLKILEDDKAWEQYEKIVDGYFSMRKELNNPLLSASKELQAIFMLDK
                     KQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEVKRVATKSLGGHGSKAYKNKSL
                     RGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLLNSQLSI
                     VS"
BASE COUNT         4350 a          955 c         1976 g         2896 t
ORIGIN      
        1 aatgaagaaa gtcaataaaa atgcttgacg tcaaatatat gacgtgttat tattaaaaca
       61 agaaaggagt tgacgtcaaa aatatgacaa acgaaagagt tagatttaca tttagattac
      121 cagcaccatt gcttaaaaaa attaaaaata gagcatcaat agaaggaagc tctatgaact
      181 cattaatact acacatactt tgggattata tacaagaagt cgagagcgag gaggcaaaaa
      241 aacatgaata atttaagtgt aattaagaat gaaggtctta taaaggtaga agttaacgaa
      301 aaacaagaac aaatattaag tgcaagagat ttacatgagt ttttagaggt tggaagtaga
      361 tacaatgatt ggtttaatag aatgattggt tatggatttg tagaaaatga agattttata
      421 tctattactc aaaaaaaagt aacagctcaa ggtaatgaaa ctgaatatat agaccatgta
      481 attaagttag atatggcaaa agaaatagct atgatacaac gtaatgaaaa aggaaaacaa
      541 gctagaaagt attttctaca aattgaaaaa gactggaata gtcccgaaaa ggttatggct
      601 agagcattaa ttgtagcaaa taaaacaata gaaaagaaaa gtagagaaat cgaggaaaaa
      661 gataaggtaa tccagttaca acaaccaaaa gtattatttg ctgattcagt agcatcttct
      721 aatgactcta tattagttgg agaacttgca aagctactta aacaaaatgg tattgataca
      781 ggcgagaaaa gattattcgc atggcttaga gataatggtt acttaataaa acgtaaaggt
      841 gaggattata atacaccaac tcaaaaaagt gtgaatttgg gagttataga aactaaagaa
      901 ggcacaagag ttcacccaaa tggatatata agtgtaacta aaacaccgaa aattactgga
      961 aagggtcagg tttatttcat taataaattt aaaagtagca aacaaatatc aatgttaagt
     1021 tagaactttt aaaattaaaa actatggagg tagtgacaat gagcgaagac atattttatg
     1081 gtataaaaaa tactcttgat gaaatagcag aggttcaaat taaaaataaa caaggtgtgt
     1141 taaaagaagt tggaatgtta ataagtggtg aggacaattc ttgtataacg cattgtttag
     1201 atgaagattt aattaagttt tacataaaag aagaggcagt attgacaata gataaagata
     1261 gtcatttgtt atgtatgctt gatgctctat tttataactt tcttgataag taataaatac
     1321 agaatattct tgaaatggtg gtgaagaaat tgggtaatat atctaacttt aatttagata
     1381 aacaagaaga taaaagtttt aatgacctgg ataatatatc aatttgtttt tcagaaggta
     1441 ttcgtaaagt tgtagaaatg aatttaaaca actataaaaa taaaatctca aaatacttaa
     1501 atgaaacttc gaaaatagaa ttgttggaac caaaagaatt aacaattgtt ataagtaaag
     1561 gttatcccga ttatcttatg tctgttgaag aagcaagtaa aagattgaaa atagataaag
     1621 tatttggata tgagttaata aaaaatggac ttttgaagtc agttgatata ggggcaacta
     1681 aagtttctag ttatgaatta gatgatttta taactaggaa tcaaggaaaa aacatcaaag
     1741 aaatgcttag agaaatgaaa gaacttagag agggggtgat ttaattggaa ctagtaacat
     1801 acagaaacaa gcttgtttta ctaaaagatg gagaaaagat tgcaactata agtttaaaaa
     1861 ggaaatttct cagcaacaga cttaaattaa aaataagata ggagagataa attgaaaata
     1921 atttataaaa acaaagttta caaagtagaa caagacaaaa agttatttag aattacatac
     1981 tatgatgagc agagaggtag taagaagttt aataaagaga agaaagtaaa aagaagtgtt
     2041 ttaacaagag atatagagtt agttaacttg tatttaccag cacatttaaa aataaaataa
     2101 gctataaata attaaagaaa aaggtgatta gatggaaata gaacaagcaa caatacgcct
     2161 gcctagagaa cttaaagaca agcttctaaa acaggcaaaa gttaaaggat atacattaaa
     2221 agatatgata gtttttattc taaaggatta tcttcaaaat atttctcaag aataaattca
     2281 atttctctac ctatagaacg cttatctttt tgagcaagtt gttcgatttt ctcaaaaaga
     2341 agtttattaa ttcttagtgt aaatctttta tcctcttcac gagtataaat atctttatta
     2401 gacatattta tcattcctca cataaaattt gacgtcttta tgacacttta ataataaaat
     2461 aatataaaag aaaagtcaac aaaaatgctt gacgtcaaat atatgacatg gtattattaa
     2521 aacaagaagg gagttgacgt caaatatatg acaaatgaaa gagttagatt tacatttaga
     2581 ttaccagcac cattgcttga aaaaattaaa aatagagcat caatagaagg tagttcaatg
     2641 aactcactta tattacacat actttgggat tacataaaag aaatagaaaa taaggaggtc
     2701 aaataatggt tgaattggta aaagaatttg atttacaaac aattaaagta ggaaatgcag
     2761 taaaagtaaa ttgcaaaaga tttggttttg aaattgattg tatagtagta gtagcaacag
     2821 aaaaagaatt aaatttagca tactttgatg aaggtagagg ctgtatggag tatcaagcct
     2881 taataacaga agatattcaa gatggtgatt atgagattaa aattttatct taggaggaaa
     2941 taaaatggta ggtttatttg caatatgttt agcaggatta tttcaataaa aaaagtgctg
     3001 gtcaaagtaa ccaacacata caaaaaattc aacttattta ggaggatacc ataaaatgaa
     3061 taaaatttca agtcgtagaa aatatttaga tgcttttata gtaactgata ctaagaacat
     3121 agataaaatt gattggctta aaaatagaca attaggaata gggggtagtg atgcgtcagc
     3181 agtagcagga ttaaatccct ggaaaacttc tgttcaagta tatatagaaa agaaagaaga
     3241 aataccaata gaaactaaaa gtttcagaat ggaattaggc aatagattag aaggattagt
     3301 tgcagaactt tttacagaag aaactggtct taaggtccgt aatgtaaatg gaatgttgaa
     3361 aaatgaaaag tatccttttg caatagctga tatagacaga gctatagtag gagaaaaagc
     3421 atttttagaa tgtaagacaa caaatagttt ttctataaaa gaatgggaaa atggagttcc
     3481 acttcattat gaaatacaat gcttacacta tatggctgtc acaggagcta cacattgtta
     3541 tatagcagca cttcttggaa atgaaaagtt tgtatggcac aagataaata gggataatga
     3601 agtaattaaa aatctaatga aaatagagag tgaattttgg gaagaaaatg tattaaaaga
     3661 cattttacca attcctgatg gttcagatgc ttatagtgag tttctgaaaa caaggtataa
     3721 aaactcagta aaagagaaaa tagaactaaa tctacttgaa gatggtatat caaagttaaa
     3781 aagatatgat gatatagttt tacaaatgaa agaactaaaa ggagagaaac agctaataga
     3841 acaagaaata caaagtgaaa tgagagagtt tgagttagct acattaggcg gaagaataat
     3901 aacttggaaa ggagctacta aaaggtccat tgataccaag agattaagag aagaaatgcc
     3961 tgatatagca gaaaaatata caaatataag ttcatacaga acattcaaaa taaaataggg
     4021 ggtaatatat atggctagtg aaaaagcaaa aggagcatta gaaaagaaag tttcaggagc
     4081 aaatacagtc aaggtaagtc caagtaaagg tatggagcaa cttatgaata aaatggcaag
     4141 tcagataaaa aaagctttac ctagtatggt ttcaagcgag agatttcaaa gagttgccct
     4201 aacagctttt agtaataatc caaggttaca atcatgtgaa cctatgagtt ttatagcagc
     4261 aatgatggaa tcagctcaat taggtcttga gcctaatacg cctttaggtc aagcatattt
     4321 gataccatat ggaaataaag tgcaattcca aattgggtat aaaggtcttt tagaattagc
     4381 acaaagaagt ggaaagataa aaactatata tgctcataaa ataagagaaa acgataaatt
     4441 tgagattaaa tatgggcttc atcaagactt agttcatgaa cctaaattaa atggtgatag
     4501 aggggaaata attggatatt atgcagtata tcatttggat acaggaggac atagtttttc
     4561 ttttatgact aaagaggaaa ttatagaatt tgcaaagagt aaaagtaaaa gttatagtag
     4621 tggaccatgg caaacagatt ttgattcaat ggctaaaaag acagttataa aacagttatt
     4681 aaaatatgca ccacttagta tagaattaca aaaagctatg gtaggtgatg aaacaataaa
     4741 atctgaaata gatgaagata tgagcatggt cgtagatgaa agcgaaagtt tagaagttga
     4801 tttcgaagta aaagaaaata tggatggtaa agttagtgtg gaagaagcta taaatgttga
     4861 ttaagtaggt gaggcacctt gaatgaagat aagtcagtta tagagaaatt aaatatatta
     4921 agtggtggat acggtcttat gccaagaata atagcaagag ataggtggtt gacagttggc
     4981 gctagaatgc tgtattcata tctaactagt tttgcaggga atgatggaac atgttttcca
     5041 tctagggatt taatttgtta tgaactagat atatcaaaag acacatttac aaagtacaaa
     5101 aaagagctag agatgagtgg ctatataagg gttcataaga ataaatccaa acaaggcaag
     5161 atgcaaaaca atatatatga aatagtattt gatagaactt atatagatga atgtatttct
     5221 aagagaggtt taaaagagga gaaaaagaag aaaaagccat gtactaaaaa gcaagacacg
     5281 gaaccgtatc ctaaaaatgt agacatggaa ccatgtccta cttttccgga cacgactcag
     5341 ccggacacgg aaaatatgga cactaatagt aacagtatta atagtaacag tattaataat
     5401 atgtatatag taaagcaacc tgtggataac tttttaaaag aatttaagaa gctgtatgaa
     5461 gaaaatatag gagtaatata tccagttaca gctgaatggt tattagaagt atctaatgaa
     5521 gtagatataa gagtatttaa aagagctata gagatatgtg ctgaaaagat gaatatgaat
     5581 ctagcatact taaaaggtat ccttaaaaaa tggaaggatg caaatattac tacatatgag
     5641 caactggaat catatagatt acagcaagaa aataagaaaa caaaaaaagt agttaataac
     5701 catgtgagta aaaataagtt tgctaacttt gaacaaacat ttactaagta ttcagagaat
     5761 gagcttgatg acattattaa gaaaagccaa aaagagaagt ttggagtagg aagttaaaat
     5821 attggaggga tgaaagatgg agttaaaatt tagagaatgg aataaaaatg gtaaagaaat
     5881 gtatagttat gatgaaatgg tgtgctattc taaaaatttg cttagagagt gggtttatag
     5941 tggtgtttat ttaccaacaa gcaatgaaaa ttttgaggtt atgatataca caggtttgaa
     6001 ggattgtatt agaaaagaaa tctatgaggg tgatattgtt tcatacatct tatcatttga
     6061 agaatttata ggagaggtaa aatttgaaga aggtttcttt gtaatagata atgaagtgct
     6121 aggagaatgc gttgggttat ttcatgaaat tgcagtggtt aaagttattg gcaatatata
     6181 tgaaaatcct gagatgttag aaaagataag gaaacctaaa gtgttggagg tctaaaagtg
     6241 aaacatgagt gtaagaaact tttcttggaa tgtgataagg gaagttttga gataaatgat
     6301 acaagaattg gagaagtaat gttcgagagt acagaaatag acaatccgtt tgagagagta
     6361 aaatatgaag gtaaagctac ttttgaaata gtatctggat gggagtatct acaaagagaa
     6421 atgttgtggc ttaagatatt gcatttatca gcagttgtag caaaaataat gcaatataaa
     6481 atgttaggta ttttaataag ggagatgtaa taaaatggct aaaatttgga tggatgcagg
     6541 aacgttttta gaaaaaacta ttgatataga agatatgttt gaacttaatt taagagcaat
     6601 aagaaagaga aatgaaaaaa taaaaaatga aattgagaaa caaataaaat atgaagattc
     6661 agaaataaaa actacaaatc gtggtggacc aggaaaaatt ataaaaattt tcaatataaa
     6721 tactggagag gtaaagattc ttaagagtgc tgaagaagca agtagatata taaaggttag
     6781 tcgtagctat gcaagttatt tagctagaga aaataaatca actgaggatg gttggaaagc
     6841 agagtatatt caaggagcga cagatggtat tagcaaatgt ggaacaagta attaagttag
     6901 ctgaaaagat attaaataag aaaaagtgtt ctgttaataa agctattgat atagctataa
     6961 aaatattaag taggtatgag tgtgagggga tgttagaaaa atgaatatat tagctattac
     7021 aatgcttata ataggaagtt ttatagctgg tagagtttat gagtatagat tgaatctaaa
     7081 agagtgtgaa aattgcgaca ataaaagagg tgtataagaa tggatgatag attagaaatg
     7141 ataaatgctt ctgtaaatta tatacagatg atatgtgaaa gttcaaatat agccattata
     7201 gcagagcaag gaagagttag aatattagat ttagaaacta aagaaaaata tgatttatta
     7261 aaaaataaac tcgaggaaat gttataagaa atataagtga aaatatctaa ttaaaacagt
     7321 ttagagagtt gcaaaatgtc ttttagtata aattattgtt gaagtgtttt gtgactctca
     7381 aaaatgaaat aaaggagtat ttaaaatgaa aaaatataaa attaagtttg aggaaaaagt
     7441 gactttagaa catgaagtta tagttgaaat tccaaacgaa ataaatataa atgatatttg
     7501 taactgtata gagaataaat gtcaaagagt atatgatata gcagattata taagagaatt
     7561 taatgggaga caaatagatt ttacagaaga tacttgtgga gagactgaaa tgttagttga
     7621 atcatttagg aaatataagg agtgatttta tgaaacgaag aagatgcagt tggtgtggga
     7681 aattatttta tattgatgaa aaatctaagg atgtttattg ttgtaaagaa tgtagaaaga
     7741 aggctaagaa ggtgaaaaaa tgaataaggt tttgaattgg ccaggtgcta aatggagcat
     7801 gtcaaaaaac atagtaggta tacttccaaa acataatata tatttagaac catattttgg
     7861 gagtggagca gtatttttta ataagaaagc atgtaacaca gagatattga atgatgcaga
     7921 taaacaaatt gtaaatttat ttaaatgtat aagagataat ccaaatgaat taatgaatgc
     7981 tatatatttt actccgtatg ctagagatga atatatgaat tgtaatatcc ttgaaactga
     8041 taatgatata gaaaaagcta gaaaatttat aataagaacg aatatggcaa gaggaggaat
     8101 acaaaattat aaaacaggtt ggaaacacac aggtcctaaa gaaagtaaaa aatgctttca
     8161 aaaagttgta gggaaatgga ataagttacc taaaattatt ttagatacag ctataaggtt
     8221 aaaagatgca gaaatagaaa atagagatgc agtagagtta ataaaaaaat acaacaaaac
     8281 tgattgttta atatatgcag accctcctta tttactaaaa acaagaagtc agaaaatgta
     8341 caatgtagaa atggaaacag aagaagaaca tgaaaaattg ctaaaaacac ttttaaagtt
     8401 cagtggatat gtaattttaa gtggatatga ttcagatttg tataatgaga tgcttaatgg
     8461 atggaataag atggaattta aggcatgcgc agaacaaggc aaaagaaaag aaatgttgtg
     8521 gaccaacttt gaaactacta aacaacttag tttgtttaaa taataggaga gagtaatgag
     8581 aattaatttt acaatagatg gagaaccagt tggcaaagaa agacctagat ttaatttggc
     8641 tactaaaagg acctatacac ctaataagac taagagttat gaggagttaa taaaatggct
     8701 gtatcaatct aaagttaaac attactttga aggctatata aaaatgactt taagatgtta
     8761 ttattctata gctaaaagta acagtaaaaa agttaaagag cagaaaagaa ataatgtatt
     8821 aagacctagt aagaaacccg atattgataa tgtagtcaag attatagctg attcacttaa
     8881 tgagatagct tataaggatg atacacagat tgttgaggtt gtagctagta aatactatag
     8941 tgataagcca agggttgagg ttatattaga ggatgttata taaaaagaat ctaaataaaa
     9001 gtcacaagaa aattattaat atactctata aaaaacgata ttaataaaat taaacaaatc
     9061 catataaaga tatgtttttt aaatggatat gtcttattga gtttttgtgc agcaaatgcc
     9121 agtaaaaaat aaatacaaag tcttattaag aaaactatta aatttcccat aaaatcctcc
     9181 taaggttaga tgatataagg ttaggatact atgagggatt gataatggca atgtattttg
     9241 tagaattgta aataaattga aataaattat tcaaatgata aaggagagat agattatgaa
     9301 tgaaaacata aataaagaaa taacagtact tggaacttta gaaatcgagg gaatgaaatt
     9361 tcatgatatt gagggtggat ttggagaaga taagaaatca atgtcagtaa aggatatagc
     9421 taagatacat aatagagaac ttaaacatgt aaatgagctt ataaataata atataaaaag
     9481 atttaaagat ggtatagata ttttagatat aaaagtgagt cgttcagagc gaccaagtta
     9541 tttaaaatca cttagtttgg aaatgggata ctctaatcaa tcttatgcaa atgcaaataa
     9601 catatatttg ttatctgaaa gaggttattc aaaactacta aagatattag aagatgataa
     9661 ggcttgggaa caatacgaga aaatagttga tggatatttc tctatgagaa aagaactaaa
     9721 taatcctctt ttaagtgcat caaaggagtt acaggctata tttatgctag ataagaaaca
     9781 agaagtctta gaaactaaaa tagagaatgt taatgagaaa ttagagaact ttatggatga
     9841 tgcgccactt tttaacattg agtgtgagtg tattgttaaa gaggttaaaa gagtagcaac
     9901 aaaatcactt ggtgggcatg gaagtaaggc ttataaaaat aaatctttaa gaggtaaagt
     9961 atacagtgat atataccatc agattaaacg agagtttgga gtagatagtt ataaggccat
    10021 aaagcgttgt cagttagata aagtattaga gattgtaaac aattataagt tacctatagt
    10081 gtttgaagaa gaaataagac ttttgaatag tcaactatca atagtaagtt aaaagaaaaa
    10141 aggagtgctt tcacactcca cttgtcaaaa atataaa
//
LOCUS       DAELJO010000143        10163 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELJO010000143 DAELJO010000000
VERSION     DAELJO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.143"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(231..488)
                     /locus_tag="KPB88_002836"
     CDS             complement(231..488)
                     /locus_tag="KPB88_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0055101.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1068..1289
                     /locus_tag="KPB88_002837"
     CDS             1068..1289
                     /locus_tag="KPB88_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG0055102.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1981..2211
                     /locus_tag="KPB88_002838"
     CDS             1981..2211
                     /locus_tag="KPB88_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG0055103.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2224..2373
                     /locus_tag="KPB88_002839"
     CDS             2224..2373
                     /locus_tag="KPB88_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG0055104.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2630..3067
                     /locus_tag="KPB88_002840"
     CDS             2630..3067
                     /locus_tag="KPB88_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0055105.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSEEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            3324..4217
                     /locus_tag="KPB88_002841"
     CDS             3324..4217
                     /locus_tag="KPB88_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0055106.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLVASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            4395..5219
                     /locus_tag="KPB88_002842"
     CDS             4395..5219
                     /locus_tag="KPB88_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0055107.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            5318..5926
                     /locus_tag="KPB88_002843"
     CDS             5318..5926
                     /locus_tag="KPB88_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055108.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            6030..6206
                     /locus_tag="KPB88_002844"
     CDS             6030..6206
                     /locus_tag="KPB88_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055109.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            7283..8035
                     /locus_tag="KPB88_002845"
     CDS             7283..8035
                     /locus_tag="KPB88_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055110.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            8025..10136
                     /locus_tag="KPB88_002846"
     CDS             8025..10136
                     /locus_tag="KPB88_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055111.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKRNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3905 a         1321 c         1279 g         3658 t
ORIGIN      
        1 cccctttata caagcaattg tatcatttta tctatttttt agccaacaaa tattttcgac
       61 cattttcttt gtattctcaa tatatctcca gtatttaaca aattttttta tagaatatac
      121 aatgtattta aattaataag tagtaaattt agttaatttg tctaaaaaaa ctttataaaa
      181 catcttcttt gtctttactc ttacatttaa caaggctatt ttcattatgt ttaaacatta
      241 ttttcattga gtattcgctt aatattgctt acacacatat tatatctaat agccaattca
      301 ttgtggttga ctccattaaa ttgttttact atatccctat ttctagcata tctaaaaagc
      361 cccttatacg taggtatata agtatttgtt cctccataca acttggcaac ctctatgaac
      421 ttatcaattc cgattatttc taccattgca tgaaattgaa ctggtatatc ctccactttt
      481 atcttcataa gttacacctc ctttaagata tttttataga cttaacaaag tttttattta
      541 atacattatt gactaaacta tatttattag ttttattgaa tctaataaaa aatttcttag
      601 ctttaaaatt aatttttgta ttaacttcaa taaaatttct cataacttat aactttgtta
      661 gctttaataa agattttaat ggtttaaaag taaaatgtta atattgaact tttaactttt
      721 ttatttacag attaaattta ataaaatttt taacataagc tttaacattt aatttaacat
      781 aaactttagt acctaacatt taactctaca catatgaaaa tccatacaaa actatctcaa
      841 ctaagcatga atatatacaa atactatttc aaaacactaa gaacatatct tcacaaataa
      901 cttgtaaact ttttcatcag caaaaaagta aaatttttcc tataaaaatt tacacaaaaa
      961 atctttagaa attactgttt aactccgata ttatcttaga tgatagtatt atagctaaca
     1021 aagaaagtcg acaaatcttt gaaattaaat ttttaggagg taattttatg gaagttgttt
     1081 caacaaaaaa tttatctagt ataaaactaa aattggatgc tggttttgat gataagggta
     1141 aggcagttgt taaaagtaaa tcatttgcaa atgtaaaagc agaggcatta aatgaagatg
     1201 tttatgctgt tgcagaagca atagcaagtc ttcaagagaa ccctattgta gatattttaa
     1261 aattagatag tacatcatta tctaaatagt atcttttaaa cgtactctgc tttacaaatt
     1321 aaaaaaatta actctttata taaaagatta agttatctaa cgcacttaat acgctataga
     1381 tttggcagat tataacttca attttggtta taactttaat atattaatgc tttgaaaaat
     1441 taatatatta aacgtatttc aacttaaaca tgttagctat tttagatgct accctacttt
     1501 aatctaatta attgaaataa attaaatatt ttctatatca gcaataacta gagtcaacaa
     1561 ttaaaatact aatattgaca attgaaatat taatactaac aagcaaaaca ttaatattga
     1621 caattgaaac actgatacta acaaacaaaa cactaatatt gacaattgaa atactaatac
     1681 taacaagcaa aacactaata ttgacaattg aaatattaat actaacaagc aaaacactaa
     1741 tattgacaat tgaaatatta atactaacaa acaaaacact aatattgaca attgaaacac
     1801 tgatactaac aaacaaaaca ctaatattga caattgaaat attaatacta acaagcaaaa
     1861 cactaatatt gacaattgaa atactaatac taacaagcaa aacactaata ttgacaatta
     1921 aaataccaat attaacaagc aaacaattaa aatataaatt attatatcgg aggtaattat
     1981 atggaaatta agaaaaaatt agtcatgagc tttaaaaatg taaaggataa acaagtttct
     2041 ttttctatag aaaatccaaa ggatgattta actgaagaaa atataaaatc tgtcatggat
     2101 ttgatagtat ctaaaaatat tttttcggtt ggaggatttg acctagcttc tgttgttgaa
     2161 gccaaggttg tggaaacaaa taccactcct tatgacttag ttatagaata gggggctaat
     2221 tttatggatg atagtttaca actgttaata accaatgttg gttttcctat agctctatcc
     2281 atgtacttat tggttagaat tgagggtaaa ttaaatactt tatcaaacag tattgataca
     2341 ttatccaaaa atattttgat atttaagaag taatttgaca atatatgtaa gtcataaaat
     2401 aggtctattt gtaaaataat tagacatatt ctatgtgaaa tactaggata tttggataat
     2461 ttatccaaat atccatagta tttttttgaa ttttaccacc tcaaatttga atttcactgt
     2521 tttatttttg aatttcacta tgcttaattt gaaatttaga acgcatctag ttggactcaa
     2581 taagatataa tgctattcta atgacagtaa taaaatgagg tgatgattaa tgaaaataca
     2641 tgtcgaacaa ggtattaatt gtgatgatat tgaaataacc ataaaatgtt ctgctctaga
     2701 taataacatt gaaaaaataa tttcattatt gagtacaccg ttacttgata taaaaggtaa
     2761 aaaatcagaa gagatttata aactcaatgt ctgtgatatt ttatactttg agacagttgg
     2821 aaataaaaca tttgcttatt atgaagatga tgtatttgaa attgatttaa aaatatataa
     2881 attatcaaaa ttattagcta atacatcctt ctcacgaata tctaaatcca tgattttaaa
     2941 tatggactac ataaaaagta taaaagtaat atcacataat cgcatgcaag ctactttaaa
     3001 aaacaacgaa aaagttatta taaatcgtcg ttatataaaa aatattaaag aaaagctaaa
     3061 catttaataa aaatctaaag taatttatta atatcactaa ttgcaaattt attgacctat
     3121 ttaataatta ataataatat aatatattca atattaaata ctaattaatt atatttagtg
     3181 taaaaataat taatcaaaat ataaaaaata ggatattatt ataggcaata actaacatca
     3241 agcgataact taatacatat aaaaataaat aaacaattaa taaaaattaa aacttcatta
     3301 taaaacggaa ggatagtgaa aaaatgcaac tatataacac cccctttaat acaaacaaac
     3361 acttaatcta tttctttaca tttacgctag tattgaactg gatattcagt cttacacctg
     3421 cgttgctcgg aattacaaat acaatacttg gagaaatcat tttttatctt gcatatacat
     3481 caccagctat tgtaggtcta ttttttattt ctacaatcta tccacaaagt gcaaaacaag
     3541 attttattta tagataccta agctttaaag aaatcggaat aaaatggata ctattaacaa
     3601 tattattttt tgctttaact tttattactt cgttaataat cggaaattac tttaatgtaa
     3661 aaacttctga tatagattgg atacatatag cagtgtttag accttctaaa atagcttata
     3721 tgctagtagc aagttttata ttgagtatac ttagtcaaga agctggatgg agaggatatg
     3781 ccattgacaa attattagtt agatttggat ttataggctc ttcaattatt ttagggcttg
     3841 tttgtggaat ctggtatcta gggtcatatt ttacacctaa tcaaatacca tataatttgg
     3901 cacaatactc cctatttgat gcttttttat ttataccaaa cattgtactt ttaaacttta
     3961 taattaattt tgtatatatc aatacaagta gaagcatttt atcagctggg ttagttcata
     4021 tgatgtacta ttttttcaac attcaattac ttctacacta ccctattaaa ttatgtgtaa
     4081 tagctcaata tgtacaaata atatttggtt taatatttct tgtatatatg atttcttcta
     4141 ataaatttaa acagaaggta aattctgaaa ttgaacagat aaagtcggat gattttgaat
     4201 ttgagttaga ctggtaattc taatatgaat tataaattaa gataatctat ggtttgatac
     4261 gattactact taaatataat ttactctcta taatttattg caacatctgg aaaataattg
     4321 tatttccaca aacgcttgaa gtatataaaa acaaaatata tactgattat aagtgaaaag
     4381 gaggtcatat tttcatgttt aaaataggag aggtttcaaa actaacacaa atttctattc
     4441 gtatgttgcg ttattatgat gaacttggta ttttaaaacc tgcaaaaaca gacaaatata
     4501 caggtcatag attatactca gttgaacaga tttcaatatt acaaagaatt gtattattga
     4561 gagattcaaa atttagcgtt gcagaaatag caaatatcgt acataattgg aatgatgaat
     4621 ttgtaattaa ggaattgaat agaaaaaaga atgaaataca aaaggaaata aagcaggaac
     4681 aacaaagaat aaataaaatt gataaattta tagaagctat aaactgtgat aaagatgaaa
     4741 ttcattacaa tgttgtcttt aaaaagattc caagttataa aatcatatca cttagagaga
     4801 taattccaga ctaccaatct gaaggtatac tctgggaaaa gctatctaaa tttatcaaag
     4861 aagaacatat agaagtatca cgtcaatcca acaataacat agctttttac catgatgaag
     4921 aagtcaaaga taatggtgta gatattgaag ttggaatggt tgtaaagaag attggaaaga
     4981 acaaatctgg atttatatat cgtgagacag aagaaattga tatgatggca tgtactatgg
     5041 tttatggtcc atatgaaaat atagctggtg catatgaatc gttctgttat tggttagata
     5101 aaaatagtga ttatcaaata tcaggtataa atagacaaat cggacataaa ggtgaacata
     5161 atgaaataaa tcctgaaaat tatcttacag aaattcaaat cccccttata aaagtctaat
     5221 agattagtac ttaaatactt aagtactaat cacccattga catcccaaaa ttttcatgat
     5281 agtatctaaa cgtgtattaa acatgtttaa tttttttatg gagaaacttt taaggggaaa
     5341 aattatgaaa gcagattcaa caaaggaaca attaattaat gccacaatcc agttactatc
     5401 ttcacataga gatgtttcaa gcattacggc tagagaaatt gtagctaaag caaatgtcaa
     5461 tttagctatg attaattatt attttaaatc taaagaggag ttaattaata tttctattaa
     5521 aaaattttta aagcgtcata tggatgaata taaggctaca catgatacta aaaatttagc
     5581 acctaaacag caattaaggg aaatactaat tgcatttttt gattttatag gtgaacatag
     5641 tcaatttata aagatttctg taccctatat attgatacag gaagaaattg tttatccatt
     5701 tgagatacta cctttaatta aggctcacta tgaaaatcag aataacaaaa aaactgaagc
     5761 tgaatacaga actattgcgt atcaaataac ttcatttatt cagttgcttt ttcttcgttc
     5821 taatgctttt ttcaagtata gtggtattga tattcttaat gttgaacaga gaaatggttt
     5881 aatagatttt caattagatt tattattggt agatattaca aaataatact atattaattt
     5941 tttatgtaca tttttttatt ttatggtaac gtttgaaaat attctcattg actaaaaata
     6001 cttgcattaa ataaggggga ggaattttaa tgttttttac atttatagga gtaatatcag
     6061 caattttatt gttgtgtaca attatatgtg gtttatggat aaaacttaat aatcgtaata
     6121 atgactctaa ttctcacttt actttttcaa tactttcaat tgctttttca ctgttaattg
     6181 tggtggcctt tttagtaaga aattaatgtc tttgttatgc tggggtttat tattatagat
     6241 ttttattatt gcatgatttt tactatatta tagtattttt attatattac agggtcttat
     6301 ttattatagt gttttgttgt tacagcattt tattatatta cagagtccta tttattatag
     6361 gatttttatt atattgtaaa atcttattta ttataggatt ttgttatatt acagactttt
     6421 attatattac aatctcttat ttattgcagt gtttctatta tattgcaaac tcttatttat
     6481 tatggtgttt tgttattgca acatttctat tatattgtaa agtcttattt attacagttc
     6541 tttattatac tacagacctt tattattaca gcatttctat tacattgtaa agtcttattt
     6601 attacagttc tttattatat actacagacc tttattatta caacatttta ttattttaac
     6661 ttctttacta taatatagta atattttaat atctcaaaat catatcaatc acttttcagc
     6721 atagtctaaa atacttccac tttctaaaat tatctatcat acctatacta ataatacttt
     6781 ttaacctttt tattatacat ttcaattgtt tttaacaacc tatttatttc caaaacccca
     6841 atatattttc tcaattacat tgtgttaata gcccctaaat cctttcaaat tatttacaat
     6901 acattctatt aaataatctt actgctctat aattcttttt ttctttttaa ccctactata
     6961 cattatttac atcatttttt tcaatacaac attagttatt ctataacctt tttactttta
     7021 ctatatactt cttttctaac tacaccttca atgttccaca tcatattact ttagcacaac
     7081 aattaatacc taaaaataac cctctaaatc ctccaataat ataatcaatt taactataat
     7141 aataacctct cacactatcc aaacaatata ttttcaaaca ttacaaaaaa acatccaact
     7201 attacaatat atctcatatc cctcctcccc tttcaaatgc tatcattgac ttacaagata
     7261 aatgaaagtg aggatgatat atatgcttaa agtaaaccaa ttatgtaaat catataaaac
     7321 aggcagtaaa acatatcctg tattaaaaaa tatttctttt gaaatcaaca aaggagaatt
     7381 tgtcgcaatc atgggttcat caggaagtgg aaaaactact cttttaaatt gtatttcatg
     7441 ttttatccca attgataatg gggaaataat tcttggaaat aataatataa gaaatttaaa
     7501 agaaaaggaa ctttcagaag ttagaaatca acaactagga tttgtattcc aagacttcat
     7561 gctatttgat ggtcttagtg tctttgaaaa tatatgtgtt ccacaaatta tcaaaggtag
     7621 tcaagtctta caaatggaaa accgtgcaat taatctatgt gagctatttg gtattgaaca
     7681 tatcagaaaa aaataccctg ccgaaatatc tggtggagaa aaacagcgta cagcagtagc
     7741 aagagcactc atgaacaacc cagatttaat attagcagat gaaccaactg gtaaccttga
     7801 tagtaagtct tgtcgtacag tcattgaatc atttttaaaa gcaaagcaag aattagatgc
     7861 tactatactc atggttacac acgatagctt tgcagcaagc ttctgtgacc gagttattgt
     7921 gcttaaagat ggaataattt ataaagaagt tgtaaaatca aattcaagac ataaattcct
     7981 agatgaatta ctagaagttt taaaagaaat gggaggcgct caagatgaca attaataaat
     8041 tatacagtaa acttcgagcc tataattgga agaactatac aactttattc ttttgtataa
     8101 tactttctgt ggtattagta acttcatatg ctctaattta ttttagccca acaatacaaa
     8161 acatacttcc aactggtgga gatagcagaa aacaggctac aatgatattc tcagttgcta
     8221 tattaggatg cgctgtcttt actacatacg cttcttccct ctttttaaaa tttaaaagca
     8281 aggaaattgg tattttcatg gctctaggaa cagataagaa gaaattaaaa aaacttctat
     8341 tttcagagat attgttaatt tcaatagtat gctgtttttt aggtcttata ttaagtattc
     8401 ctgtatcttt tggtatatgg aaaatatttc aattagtgat tattgatacc agagaaatga
     8461 catatcattt tggaactcaa ggattcatat atggatttat atttagtgta tttgtgacat
     8521 tatgcatatt tatactttct gtaaaattta ttaatcgtac aaatataatt gatatattaa
     8581 atgaccagag aatatctgaa ccaatacagg atgtaaagga ctggtatggt atattaggta
     8641 taatttttat tatactcggt atttttctag gatatctcgt accttcacta actataaaca
     8701 aattaaatta tagactacca tctgtatgga gctttactta tctactctct cttattggaa
     8761 tatatatgat tatgacatac atagttgtac atgctaaaag aggaaaacat ccagagaaat
     8821 actataaaaa catcatatca actagcatga tgagatttat gggaagacaa acagtaaaaa
     8881 acatgtgtgt tatttcattt ttgattgcag gagcattatt tgctgtattc tatgttccta
     8941 atatggttac aggtatctat gatactctta aaaataaccc tatagattat acttttacct
     9001 acaatcaaag agataaacag gtatcaaaaa aagatattga acaattagca gataaacaca
     9061 atataaaaat tcaaaactat atggaaattc cttctataga attaattgtt gatggtgtag
     9121 aatcaatata tcataaagat ggaactatcg aaaaggtata tatgaaaaaa agctcctatg
     9181 atatgttctt taattctaaa gatttttcca agctaattgg caaaccaata actataaaaa
     9241 atggtgaata tcttgctgtt gtaaataaaa gaaatgaaaa tcaagattat aaatatacca
     9301 aatttatatc aagcccagtc acagaaaaag aaatcccctt tagatatgct ggaaaaataa
     9361 tatttaatgg ttcttttttt caaaatagaa gtatgaacgc ctatgtcctt aatgatgagg
     9421 attataaatc atttactaaa gatttatcca taaaaaatta caataattct atactattta
     9481 atgtagataa agatacctat gcttttgcca atgacttaaa gaaagaaatt ataaaaagaa
     9541 catctaagga aatgggcatg ttcagtggtt atgatgaata cgtaaaaaaa cgttatgaat
     9601 caactggaag aaaatatttt atggatgaag aatatccaag tggtgaaggt catttaaaac
     9661 tagacccaaa caacaatcag ttgtatctta attggaaata ctatcctaac tttaaaattt
     9721 tacaaagcca agatatgata aaaaacatgg cagtcttctt aatgttattc atatatgtgg
     9781 caatcatatg ttttacagca gtaggaatca ttgcatatac aaggggtgtt actatagcta
     9841 taaatcacaa gcaggtcttt atggatttaa atcgattagg agcaaacaat gattatataa
     9901 agttctgtat tagaaatcaa ttaaagaaag tattttcata ccctcttatt gcaggaagtt
     9961 ttttaattta tctgtttaat tttatgatta tgttaaataa tgatggtaga atcgtcaata
    10021 gtgaggtcaa tgctcttttg ataaacttag gttttatagg gatttgtggg gtttatatgt
    10081 accttatata tctattaact tataagaaat tcaaaaaaat tattggtatt ttataataaa
    10141 aaaggttatt agatttttaa agt
//
LOCUS       DAELJO010000144        10149 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELJO010000144 DAELJO010000000
VERSION     DAELJO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.144"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(11..643)
                     /locus_tag="KPB88_002847"
     CDS             complement(11..643)
                     /locus_tag="KPB88_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0055112.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(715..1068)
                     /locus_tag="KPB88_002848"
     CDS             complement(715..1068)
                     /locus_tag="KPB88_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0055113.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1146..1349)
                     /locus_tag="KPB88_002849"
     CDS             complement(1146..1349)
                     /locus_tag="KPB88_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0055114.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1739..1969)
                     /locus_tag="KPB88_002850"
     CDS             complement(1739..1969)
                     /locus_tag="KPB88_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055115.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1971..2378)
                     /locus_tag="KPB88_002851"
     CDS             complement(1971..2378)
                     /locus_tag="KPB88_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0055116.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2738..3187)
                     /locus_tag="KPB88_002852"
                     /pseudo
     CDS             complement(2738..3187)
                     /locus_tag="KPB88_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3201..4127)
                     /locus_tag="KPB88_002853"
     CDS             complement(3201..4127)
                     /locus_tag="KPB88_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055117.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4252..5034)
                     /locus_tag="KPB88_002854"
     CDS             complement(4252..5034)
                     /locus_tag="KPB88_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0055118.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5190..5810)
                     /locus_tag="KPB88_002855"
     CDS             complement(5190..5810)
                     /locus_tag="KPB88_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0055119.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5898..6596)
                     /locus_tag="KPB88_002856"
     CDS             complement(5898..6596)
                     /locus_tag="KPB88_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0055120.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6697..7512
                     /locus_tag="KPB88_002857"
                     /pseudo
     CDS             6697..7512
                     /locus_tag="KPB88_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8183..8950
                     /locus_tag="KPB88_002858"
     CDS             8183..8950
                     /locus_tag="KPB88_002858"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0055121.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9283..9960)
                     /locus_tag="KPB88_002859"
     CDS             complement(9283..9960)
                     /locus_tag="KPB88_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055122.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
BASE COUNT         3389 a         1608 c         1476 g         3676 t
ORIGIN      
        1 ttataaaata ttaaactaat ttatcaatat ttcctgattg aatatgctta atgtttgatt
       61 gaattttttt atatggccaa tcccaccatt taatttctaa taattttgaa attgtatcat
      121 tattaaatct cttttttata atttttgcag gtactcctcc aacaatagaa tacggtggaa
      181 cgtcattagt aactactgct cttgttccaa taattgcacc atcaccaata tttacacctg
      241 acataattat agaatcataa cctatccaca catcatttcc aattataatg ttacccttat
      301 tgtcccaagc gtccgttata tggttgatat cgagtttcca ttcttcataa aagatgggaa
      361 aagtatatgt ggattttgat ttcattgagt gatttccact agtcattaga aattttgcat
      421 tacatgctat tgagcaaaac ttaccaatta ttaatttatc attgtttata gggtaatgat
      481 acaatacatt gtttttctca aaatctcttg ggtcattata aaagtcatta tatattgtgt
      541 aatctccaac ttctatattt tcccttgtta ttacattttt aagatataca gtttgataat
      601 cattacttct tggatatacc ttatttgaac ttggaatagt catatttaaa tctctccttt
      661 aatatatatt atgtttttga ctattccact actacaatac atctcttcac agaatcactc
      721 cttgttaaga atcttcataa gataatggcg tcctttttta gtgttaagtc ggatattctt
      781 tcttagtcca ttctttttag cgtataactg acaaactgtc attttcttac caatagtgtc
      841 aatgctatca tcaaggtcat tttgtattgc tttttcctta tcccttaatg atatatcatc
      901 acgttttccc tttggaggtt tatcagtagg tacaagtttc caagagtaaa caaattgtgg
      961 tttaccaaat gaatatacat acttatatgc gtatcttcca tcttttctct ggctctctcc
     1021 agttcttaaa attctatctt tattatctcg tcttttttca gttgacatga taagaagctc
     1081 ctctcaataa ccgaattgag cttgatacgt catatgaata ttataacata taaggctcaa
     1141 tttcattaga taacgtcaag tgtatcaacg attttctcaa attgttttct ctttatttga
     1201 atgcgattgc tattcataat gacccagcca gaatcaaggt tttcatcagc acgtttacgt
     1261 aattttttct ctccaatacg aaagtatttt gacgcttctt caatcgtcaa ggtatatttt
     1321 tcccaaatag gtacatcagt attgttcata gaatgtatca ctcctttcta tgttcgtagt
     1381 tcagaagtag atatatggct ttggtaagct ccattggtat ttcaaccaat cccattctta
     1441 tgggcagaac cgtgtcatac aggtgtatca ttatactgtg gtacaggtta cggcaaaaca
     1501 accgttccaa aggttattca caaatcacta tatagtagct ctcttgatta aggtcatggt
     1561 gtatagtatt tgttggctat ctgtatcaca aacgtatcta tctactttat tcagttgtca
     1621 aagaacagtc ggcattgatt tgcctataaa aaatcacaat agcttgtggg cttttataga
     1681 caaataatta gtgattctcc atgtaccaaa tagcttgata catggagaga gggcgagatt
     1741 aagacaaatt aaatctcaaa atagcagtga ttagttttgt tcctagcatt cgctttgttt
     1801 cttcttctac atgaatatag ggatttccgt attcatcaaa taaagttttc tgtgaaagac
     1861 gaatgatata gtgttcaaaa tgtttaagta ttacgtttat tgcatttaca tcgccatgta
     1921 cagcaagtaa aataattgta aaaggtaata cttctttagt aggtttcatt tcaatgttcc
     1981 tgcatgagtt ctttcaactc ttttagtgca tttgttttgt gataatgtat ggtattttct
     2041 gctaaatcca tcaatgttgc tatgtcagca tttgtcatgt tgagaaagaa tgaaagtaga
     2101 attatatctt gttttctttt tgtaagtttt ccgattgctt gcgccattaa tacatcttca
     2161 attcgtatgt tgtagtcgta agaaaccata cagatgtagt tagaatcata atcatctaaa
     2221 atactcaatt tacttaattc tgcaagtgac aaagattcga gagataccaa ataatcattt
     2281 ctgcgtttat tttcgtcata aatatctcta gcatagttac gcaaaactgt tttacagaaa
     2341 ctgtcgaatt gatgttcaat tgtcttttca tcaaccataa gatttcaccc tctttccatc
     2401 gcaaggagtt aaataacctt gcttactcct atcacggtgg gagggcgtta aatcttcgat
     2461 aaatttcaaa aaaaagagaa aaaaatacac actctgccta tagggcaaaa atgtgtattc
     2521 tttaaaacgt atttaattta tattggttgt aagccaccac catattgatg tatggaaata
     2581 tgcattggct agcttttcat aaaaatacaa agatgcataa gaaattaata gaggggaaaa
     2641 acttattctg ttattaaaaa gtaggttaat tctaataaat gctattgcgg tcgaattaaa
     2701 ctatatacat ggcaatcaat catgtttcta ttctttatta tgaattgtga cagtattctt
     2761 caatcttaaa gccacatttt tccaatactt tacttgactc tctattgtag gaatatacat
     2821 tagcatgaat tttgttaagg ttgtattttt caaaggcaat tttgcatatt tcttttactg
     2881 catttgttgc tatccctttg cctcaatagt tcctagcaag ccagtaacct aattcagcat
     2941 ttttattaat agaggtatct tcttcaaatg ttaggctgat acttccaata gccctttctt
     3001 gataaaaaat agcatagcta agttgtttaa catctgaagt tgccaaacaa aaactaataa
     3061 aatctctagc gttttgctcc ctataagggt gggggaagtt gtcacgaaga ttctttgcaa
     3121 tttctttatc attcgcaaac tcaactaaat cggtaatata agcataattc cactctataa
     3181 gttccatttt tcttctcctt ttatgaaaca gctacaagaa atctttctaa gccaatacca
     3241 cttatcaaac agcgttcttt tttatttcca agcatttgat aagtccagtc aacaaatcca
     3301 ccgtcaacta tttcaacggt ttcatttcct tgtttgaccg ataatttgaa attgatacct
     3361 ttataataat tattatcaac atcatcatta tgaattgata actctatatt aggaaaaata
     3421 agtttgatag tttcaaccat tctgtcgaaa aatccgtcat tatctttgta tccgtttctt
     3481 ttgcgtagaa taatagagaa acgaacattt tctatttcac ttaatatatc tttataatag
     3541 tttaagtgct tttctaacaa cattttttca caagtgtaag accctgtatc tataccagat
     3601 gaaaccatac aatataagcc aaaatgtgaa tataaacctt tacctacaaa attttgtgct
     3661 cttattaccc tagcagtagt agccatgtgg ataggtatgg tattattttc ttcttttctt
     3721 tttaatttat cagcaataat tattgccaac atatttgagg ggtctgcaag tgtttctgtc
     3781 cctcgtagag cacttactac gttatattga tctacggagc caaaaacgct acagctacct
     3841 aatggggcag agggagaaag cataatcgtc ttgatatcca tattgaatgc ttttctcaat
     3901 aattttgatt ccaaagcatg aatttcttct ggatctatac cgctagggat agtaaatcta
     3961 tttagttggt atgactttag aatatcagtt gcagttaaag tatttgcttg tctatcaaac
     4021 acttctaata gaagtgagtt caaatctgat tttgatagag ctgataactt atcaactata
     4081 ttcttatccc caattttatt tgtaattcta cttaaaatat cactcatagt tatcctccat
     4141 actgttactt tcaaaataat tatagaattg ttacaacaac taatctaggt catttgacca
     4201 aaaatgaaaa aacactgaat atttactatt caatattttt ttactttggg tttatttgat
     4261 taaatcttga aaatactgta atgcaaccct ttcagcttca acctttcgga caataggact
     4321 ttgtgataat tcctctctta actcaagata caggttgata gtagcctgca atgcattaac
     4381 acagctttta tattcatggt tagcaaccgt cttttctaat gcttcaatat attggggtgc
     4441 gtccaattca atttttctaa ctccacgagg taatttacct aagagaagct gtattaatgg
     4501 ggcaagtact gattgtctta aaaaagataa aaaatcaatt gtttcaaata gttctctacg
     4561 tccaatctta gtgcaagcgt aatgaaccca aatccaaaat ctatcttcaa tccactgtaa
     4621 gtctggagtt gggaagtatg cttctttttt agaataaatt tctgttatgc agttgttttc
     4681 ttcatagaga atagctgaat cttcaaccct atctagtaaa ccatctaagg ttgtaaactt
     4741 aaaatcaaca tgaagaacag gggaatcata gagggaaatt atcaatcgag gttctccaac
     4801 gtgttcgcca gtaaaagcag aaactaatgt tccaaactgt tccacaataa gtaatctctc
     4861 ttcgataact tcttcatagg atttatcatc tgctacaatc agaaaatcca aatcagaata
     4921 ttcatctaac gattttgtga tataagaacc gctaattgca agtcctatta tcctactatc
     4981 tctttttgaa aattctaata cgttttgtat cattttctgt tgtgttttag tcataatata
     5041 acacctctca ctgtctttta ttagctatca atccaacata attatgcata ggagtattat
     5101 ataatgaaga aataaaaaaa aataggtcac ttgacccgaa ataaaaaagc attgaataat
     5161 tattattcaa tacttttttg aaattatact tatgttgtag gaagattctt taatacctct
     5221 cgctgattaa ttacactaaa tcttttgttt tcataagcaa ttagatgttc ttgtacacat
     5281 tcatttatag cacgattaat gcttctaatt ggagcgttta cttcgctagc aagctgtttt
     5341 ttggtaagca gatttaattc acttctataa tagtgtaagg atatacacct cagaagtcgt
     5401 tctttaacac ttaaagatga taattcttca attaattcag catttacaac taatttacta
     5461 gatatttctc gaatcagaaa ttttgtagct tcaaaatctc cccttaacca gtttaaaaaa
     5521 tcatctctat gaagtttata tacattacaa tctgtgaaag cgacaatttc aacgggtttt
     5581 ttcccatcat taaattgttc catttctcca aaaaaactac cacttctata aagatgaatc
     5641 gttgagatgt ttcctttcac atttaagatg aatgcttctg caataccgtc aatcataaga
     5701 attacatatt cattttcttg ctcagcaaaa agaatagtct ctttcttttt atacgttttt
     5761 ttaacaagct tctttttaat atcttcattc ataaaatcaa ttaaatgcat attcatccct
     5821 ccttaccaaa tctttgatta aattatatca tctttcaatt agttaggtaa gatagtaaaa
     5881 atatatttac atagaaacta ttccattatt attttccagt tgtcattttt ctgcaaaaac
     5941 ttatcactgt cacgactgga gataccagta aggaatgtta ggatatcaat cgctaaactt
     6001 tcagaatcct ttttacggct caaaatgacg taagggtcaa gcaatccttt gttttcatca
     6061 ttactggtta aattgacaat gtttatctca tgggctattt ctggtagcgt ttctttccat
     6121 ttgccgcgtt ctgctttagg gtcaactatg accgcctgtc cgccgaatag taccgcatag
     6181 taaacaagca tattgttact gaataattta ccaccaccca atgaaccgat aaacgcagac
     6241 gctaacgcat tggtaataga accctttacc ccttgactag caagggcagg ttttagatag
     6301 acattctttc cagtgtccag attgtagcca agatagatat cctcatgttc gccaagggtt
     6361 tgtgtcgctc caaaaccaag tccagccaaa aaatccgaag tgacatattg aatataatca
     6421 ttgatgtaac gtttgctgac aggaataaat tcgccatgca atcccaacat atccccaaag
     6481 ggacgcacga gcttgattga taagtcgtcg taaaagtctt tgacttcatc acaacgcctt
     6541 tttagctcgt caagattctt tgtagataca cggacaacat agctaagttt atacatagat
     6601 tctttacagt taaaagtctg tttttaaaaa aatgtataaa ttataaatca aaatatagat
     6661 actatcaatg acccactagg tgcaaaaagc attgatatgg ttaaattatc agacgctaaa
     6721 agctgggcta tccgaatgag tgaagatggg tgtgcttata aatccattga caattataag
     6781 cgctcactga aagcgtcgtt tttcatggca attcaagacg actgtattag aaagaatcca
     6841 tttaattttt agttaagtga tgttttggaa gatacaacag aaccaaaaat cgctctaaca
     6901 ccagaacagg aaatgggctt attatccttt atggggagcg ataagactta ttgtaaatat
     6961 tatgatgatg ttgttatcct cttagaaaca ggactacgca tttctgaatt atgcggtttg
     7021 actttagaat tggatatgaa aaataaagcc attatggtag accatcaatt gttgagtgat
     7081 actaagattg gctattatgt agaagttcca aagaccacac agggtaagcg agaaattcca
     7141 atgactgaaa gagcttatca atcatttgaa cgtatcgtgg aacggcgtaa gaacgctaaa
     7201 gcagaaccaa tcattgttga tggttaccag aacttcctat ttcttaaaca agatggatta
     7261 ccaaaacaag caactcatta tcaaagcatg ttaagagcgt tagtcaagaa gtataacaag
     7321 acccatgagg acaagctacc aaagattacg ccatatatct ttatgcatac actctgtaca
     7381 aatatggcta acaaaggaat gagtcctaat atattgcaat atattatggg gcataaaaac
     7441 attactatga cgcttggcta ttatgcacat ggttcattta gttctgcgag agctgaaatg
     7501 gaacggttat aagcctagta gtatgactag agtttactac ttatttacta cttttgatgg
     7561 cattcttata cggttaaatg ccaacttatg caaagtatct tccaaagaga aaatgccgat
     7621 actccttgta gatacaggat atatcgacat ttaccaactt atgaaaagat aatcaaattt
     7681 aatataagtt tttcaaataa aaagatacta aattcttttt agagaatcta tataacatta
     7741 cttaagcttg gttggggtat aaacaattga gtagctactt ttgtatggtg ttcaacttta
     7801 gtcaaaactc ttaaataatg caaatgattt agattcatta atattcaatc tcttcataag
     7861 tagtgaaatt cgagttattt ctgcataatt atactatgat ttatagatta attctattag
     7921 catccagaat aatattgttt atatgagtaa aatttaaaga atattgtgaa aattatagaa
     7981 tgttaaaata aatttaggta aaaaaaagtg tttgtatatt atagagtacc ttttctaaaa
     8041 tatgtgggat tgttttaaga attagaataa aaattatttt taaattagta tttttataat
     8101 gtatagtatt tttaatgtat aattaaattg tgcagaaaat agaatttaaa gtcctataga
     8161 atttctgatt atggaggaga atatgaaaag aaaagttcaa gtaaaaaata ttactattgg
     8221 agaaggtaga cctaaaattt gtgtaccaat tattgggaaa aataaaaaag atattataaa
     8281 agaggctaaa gagttaaaag atgcatgttt ggatattata gagtggcgag ttgatttttt
     8341 tgagaatgta gaaaatatta aagaagttaa ggaagttctc tatgaattaa gaagttatat
     8401 acatgatatc cctttattat tcacatttag aagtgtagta gaagggggag aaaaattaat
     8461 atcaagagat tattatacta ctttgaataa agaaatttct aatacaggtt tagtagattt
     8521 aatagatgta gagttattta tgggagatga ggtcatagat gaagtagtta attttgctca
     8581 taaaaaagaa gtgaaagtaa ttatatctaa tcatgatttt aataaaactc caaaaaaaga
     8641 agagattgta tctcgtttat gtagaatgca ggaattgggc gcagatttac ccaaaatagc
     8701 tgttatgccg caaaatgaaa aagatgtgtt agtgctactt gaagctacaa atgaaatgtt
     8761 taaaatttat gcagatagac caataataac aatgtcaatg tcaggaatgg gtgttataag
     8821 tagattgtgt ggtgaaatat ttggttctgc attgactttt ggagctgcaa aaagtgtttc
     8881 agcaccagga caaatatcat ttaaagagtt aaattcagta cttaatctat tacataagag
     8941 tattaattaa attaattata tagaaatatt agaaggtatt ttatttttga agatataaat
     9001 taaatataaa aaacttttca gatagtaaaa tttaaattgg gcaattatat ctgaaaagca
     9061 actatttagt gtgtattttg aaagtgatat agaaaatctt ataaatcaac tatggcatat
     9121 ttaaaatatg ccattaaaac ctactagaca ttagattata gatggtatac tagcatttct
     9181 attatttata gatagtaagt tttcataata actgttttat ctaaagtaga agatatattt
     9241 gtattatgta tatttgtcat tatttttttg taattatgta tattaatggt tatatatgta
     9301 atataatatt atcactctat tttttgtata ttgtacatta caacatttat atctatcatc
     9361 atcaattaca gtagaatgta tgggatactt attttcactt aaccaatatt tttgcatatt
     9421 ctttgtccaa gactttggaa acttcaatac ttttttgttt attataacat cctcatcttc
     9481 aaaaatatta acttctcttt ctatttttat ttttctgttg tatatcttat tgatagattc
     9541 tttgtcgagt tttatgtccc tatacatatc agggcaatag caacaaacat aatttctttt
     9601 ttttcgacgt ttaaatttca caactttctc tatgctaatt tctaattttt ttgtaccatc
     9661 ttttataaat ttaatatctt ctatgttctc gcttgaaaaa atttctgtca atcttaaagt
     9721 atctacagtt tctctaatta tttgcttttt ataagaagag aatccatatc caaaagaaag
     9781 gtttaattta ttagggatgt agccattttt atatttaaac atcttaattt tttccaattc
     9841 agcattatct ttaaatttca acttttcata cctaaaagtt actttgctaa aaagagtata
     9901 ttctacatca atatattctt ttgcaatctt aaatttatcg ctaccttcaa tcacaatcat
     9961 agcctatctc caatcgtttt atattaaaat aaggccatag aacatggtct aatagtactc
    10021 attagcacta taagacaatg ttaacacaaa aagacaaaaa aaggttattt atttcaaata
    10081 accttttttt ttatataaaa tataaagaat tatattttat tcttttttat gatgtctata
    10141 atctgtgat
//
LOCUS       DAELJO010000145        10037 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELJO010000145 DAELJO010000000
VERSION     DAELJO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.145"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            157..1308
                     /locus_tag="KPB88_002860"
     CDS             157..1308
                     /locus_tag="KPB88_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055123.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRMRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVNFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1426..2064
                     /locus_tag="KPB88_002861"
     CDS             1426..2064
                     /locus_tag="KPB88_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0055124.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTVKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2065..3087
                     /locus_tag="KPB88_002862"
     CDS             2065..3087
                     /locus_tag="KPB88_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG0055125.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3077..3697
                     /locus_tag="KPB88_002863"
     CDS             3077..3697
                     /locus_tag="KPB88_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055126.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3821..4507)
                     /locus_tag="KPB88_002864"
     CDS             complement(3821..4507)
                     /locus_tag="KPB88_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG0055127.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4521..5066)
                     /locus_tag="KPB88_002865"
     CDS             complement(4521..5066)
                     /locus_tag="KPB88_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055128.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5293..6540
                     /locus_tag="KPB88_002866"
     CDS             5293..6540
                     /locus_tag="KPB88_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0055129.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVNFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGVLMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6627..8621
                     /locus_tag="KPB88_002867"
     CDS             6627..8621
                     /locus_tag="KPB88_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0055130.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8621..9943
                     /locus_tag="KPB88_002868"
     CDS             8621..9943
                     /locus_tag="KPB88_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0055131.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         4074 a          992 c         1663 g         3308 t
ORIGIN      
        1 tttaactata aattaggttt atattttata aaaatttgct aaaaaaaaca attaaaaatt
       61 gtaaagaaaa aagatataat tagaactgta gtaataaatg ttttattata gataatagag
      121 ttttaagtat cttaaataat atttggagga atatgaatgg aattaagacc aagaagtgca
      181 aaattagaag aatttgataa agtaatagaa ttaataaact atgtatttag aatatcaaga
      241 aatcataaac caactatgat ggaagaattt cctcttttac taagtaaaaa taatatagaa
      301 aatatgatta taatatcaga agatgataaa gtggtttctg atgtaaacta tctaattcag
      361 gatgtatcta ttcaaggaaa cagattgaag gttgcagcta taggtggagt atgtactcat
      421 ccagattatg aaaagagagg ctattcatct aagatattgg ataaagtaga agaaaaaatg
      481 ttttatgatg gtgtagatat tgtaattata tcaggaacaa gaagtctata cagtagaaga
      541 aattgtagtt tggtaaagag tttttataaa tatacaataa aacctgaaga tgtaaaaata
      601 gcatatgaaa tagttgaatt tgatgaaact aattttgaaa aagataatga tttagataaa
      661 atgattgaac tatataatca aaatagtact agatttataa gaatgagaga tgagtttcaa
      721 aaactattgc atgcagcaac tattgcctgg ggaccaattg gttacaagaa ggtatttata
      781 aaggaaaata ataatataat aggatatttg ataataagaa caataaaaaa agaagattct
      841 actgtaggag aagttgctga aatagggtta aactctgtaa atgttgaaaa tatattgaaa
      901 tatgttgcaa ataaatttgg attagaatat ttaaattata aagtacatgt gaaaaatctt
      961 aaagaccagt tgaaatgtaa tgggacaaaa tctttagact atcaacaagg aactatgaaa
     1021 ataataaatt ttactaagtt gtgtgatagt ttaagaagtt attttagtca atatgtaaat
     1081 tttgaattac taaaatacat ggaatttaaa caagttgaaa ataaatatat tattaaatac
     1141 aaagaagaag aacttgtaat agaaaatttg gataagctta ataaactgtt ttttgaaaaa
     1201 aatgaggaac agtacaatga atttaaacat ctaaaaaata tatatgaatt tgctacaaag
     1261 gcatttcctg tagattttcc atggactgca aatttaaatt atcaatagtt ttagatgata
     1321 ttaaataaaa ttgattgata cctagagtta gtccttgtta aataagagaa attattggat
     1381 attgaaaaag caattttgta agtaattaga aaggatgagt attatatgga catacttgat
     1441 gatatagcag aatgtatatt aaatatgggt gtagataata tagagaattt agtaaaaatt
     1501 gctatagata aaaatattga tgtggaagat atttatgaat atggtttaaa caaaggtatg
     1561 ataggagctt tagataagtt tgagaataag gaatactatc tttcagaagt tatagtatgc
     1621 acagatgcat taaataaagg tattaatctt ttgaagggaa ctgtaaaagt aaagaaaaaa
     1681 tcaaagggag taattctgat gtctgtagta gaaggagata ctcatgagat aggaaaaaat
     1741 attgtaaaag taatggtaga agccactgga tataaggtta ttgacttagg tgtaaataga
     1801 aaaagtgaag atataataga agaagctata aaaaacaatg tagatataat aggcttatct
     1861 tcaatgatga caactacaat ggaaaatatg aaatcggtta tagacgaact taatatgatt
     1921 gagatagata aaagaccaaa agtaataatt ggtggaggac ctgtaagtat ggagtttgca
     1981 gaagaaatag gagcagatgg atacagttca aatgctccaa aagcagttaa attaataaat
     2041 aaacttatag gaggtgaagt ttaaatgttt tcagatatga ctttacttca gtatataaat
     2101 agtgaagata gaggtttttt tctgcctgat atgggtacaa atggattgtt cttaataaat
     2161 aaaaaggcat atgaagtata tgatagtgta gatttgcaat tagaattagc aaagactatg
     2221 gacaagtatt ttgaatcaga ttttatatac tcattctgtg atgggattac attttgtgaa
     2281 actctgggtt tagagacatt aagaccagat tatgattttc caagtgtatt gacacatact
     2341 ataactgaca gagataaatt aagaaaatta gatgttccag acccttatac gagtggacgt
     2401 atgccactca atatagagag cttatcagtt atatcaaaaa gtattaaaaa accactatat
     2461 gaatcgatac aaggtccatt tacacttgca ggtcaattag ctggagctac tcaactttta
     2521 agatgtataa taactgataa aaaatttgta gaagaattat tggaatttac aactgaattg
     2581 gtaagaagat atgctgtagc tgcaaataaa gcaggagcaa aatatatttc aatagcagag
     2641 ccaacatcag taaccctaag taaagataga tttgatgaat ttatagtaaa aaatttaaat
     2701 aaaatctaca atgaacttga ttgttggaaa ggtatgcata tatgtggaga caccagagaa
     2761 ttattagata atatgctaag ttgtaatata gatgcagtaa gtttagacca aattttagac
     2821 tatgaagaaa ttgctccaat gattcctaga gatatagttt tgattggtaa tttagaccca
     2881 ataaaacttc ttggacgttc taagccagat aagattagaa gagagacgct aaaattgtta
     2941 aagaagatga gaggatatga taacttttta tgtgattttg gatgtaactg cttaaatacc
     3001 actccagtag aaaatttaca agcagctata aaggcaggaa gaattagtta taaagaatta
     3061 gataggattg atattgatga attataatag taataaaaat aaatatcttt taaaggatat
     3121 aaatttagat gatattttta tagataagaa tattgtgcta aagtttttgg ggtatggaaa
     3181 tagaaaagct ccagatacaa ttagtaaaat cgttgaaaga gaaataagaa atataaatga
     3241 tattttagat atcaagatat atataagtga agttgatatt aataaaacac ctaaagaatg
     3301 taaaaaagca tttgctgtac tatatacaat aggggataaa attgatttta agatgaatga
     3361 ctatatggaa aattgcaata tgatggcagg gctagcattg gataaaatag gtatagtatg
     3421 tttagattat attaatgaga aaataaaaat gtatttaaaa ggaaagtatg atagtctcaa
     3481 aatatcgcat gaaatatatc caggagataa agattttgaa gtagagagac aaaaggatat
     3541 ttataactat gtaaaaaata aatataatag tatagaaata gaaatcaatg attatcatca
     3601 actatccccc ataaaaagtg ttgcaatgct tattttaatg ggggatgaag aaaatttaga
     3661 atcaagatgt agtaagtgtc ctagaaaatg cttttagtta attttcaaga ctaagatttg
     3721 ttttgtgtat gaattgcaat aatttttttg gtcattttat gtataaacta aaaaaattat
     3781 tgcaaagtaa tattctattt aatttgaaca gacttacaaa ctatagtagt ttctttaatt
     3841 caataagtgt atttatttca taagttggta tatgattagt ataatttata ctgttattgg
     3901 aatttaacca acaagtatct attcctgaat ttattccacc taagacatca gaggttaagc
     3961 tatccccaat cattaaggca gaagatttat catgacaatt aaactttttg agagcgtatt
     4021 taaatatttc aggatttggt tttgatattt tcaactcttc agatacaacc attccatcaa
     4081 tatatccttt tatttttgaa ttttcaagtc ttttcttttg aactgcagaa attccatttg
     4141 ttattataac aatagtatac ttttttttaa gatattgtaa tatatcgatt gcacctggaa
     4201 ttaaaaatgt acattcacct aaaaaattta agtacatttt gctaagagtt tcagaatcaa
     4261 catccaaaga tattttattt gcaaaaagtt caaatctaag taatttaagt tcattcaaag
     4321 ttataatatt tttttcaagg tcgagccaaa gcttatcact tatatttcta tatgactcta
     4381 tataattctc aaaattaaat tctaaatcaa actcagatag aagtttttta aaagcatgtg
     4441 attcactttt cttaaaatca aataaagtat catctgcatc aaaaaatata aatttatatg
     4501 tcttcatggt ttttactcct ttacaagatg actgcaaaaa ttactttact ttcactattt
     4561 gagtcatttt gccatttatg ctttgttcct aaaggaactt ttacactatc tcctttggac
     4621 aaagtaaatt catcatcatc cataaaaagt ttaacttctc cctccaaaac atatgcaatt
     4681 tcatctccat tatgagtaat cctatcaaca caagaagaag tgtttggagc taaatccata
     4741 attgcaaatt ctaaattacc actagagtct ggaattaaaa tctcatatat aacttctttg
     4801 cttccaggta aaataacttt ttttctatta tcatgtctta ctatcaagtt ttttttatct
     4861 acttcagata caaaaaatgt aaataaagga acgtttagag aagaggctat agattttaat
     4921 gaatttacag atggattagc aagacctcgt tctatttggc tcaataatga tggtgtaaca
     4981 tcagctagtt ttgctaaatc tcgaatactt aagttttgtt tttttcgaac ttcagctatt
     5041 ttttccccta aatttaaatc attcatataa atatcctctc tttttaattt aaaaatatta
     5101 acttaaaata atcaagtaaa atgattatgt agtatgaaat tattataaca tatagaatta
     5161 ggttttgaaa aaactatgtt caatataatt aaataaatta aaatataatt gacaaagtgg
     5221 atacagaata cttaaaatat atttaaataa aattaagcaa aagatagtta atttatgata
     5281 ggaggggata ttatgcaaat aacgactttt ggagctatat ttggtttatt aatagcaatt
     5341 attttaatta taaaaaaatt tcaagctgta tatagtttaa tgttaggagc atttattgga
     5401 ggattagttg gaggagcaaa tataacacaa actgtaaatt ttatggcaaa tggagctatg
     5461 aatatatcac catcaattct tagagctttg gcatcaggtg tattagctgg aagtttaata
     5521 aaaacagggg cagtagataa aatatcagaa cagattgtaa aaatatttgg tgaaaagagg
     5581 gctttatttt cgattgccat ttcaactatg gtacttgctg gagtaggtgt aaatttggat
     5641 gtatctataa taactgtagc acctatagga ttatacatag gtagaaaatt aaattattct
     5701 aaattgtcaa tactattagc tatgttggga gggggtaaag caggtaatat aatatcacca
     5761 aatccaaata ctatagcagt agcagataat ttttctgtca atctctctag tgttatgatg
     5821 gcaaatataa ttccagcaat tattggagtt gttattacag ttattttagc aagtatatta
     5881 ataaataaag gaaacaaagt acaatcatat gaaattttag agcaaagaga agatttacca
     5941 agtttattta aatcattatg tggtccaatt atagctatat ttctattgtt tttaggaaat
     6001 gtatctccta tagttataga cccaatgata gcccttccaa taggaggaat cgtaactcta
     6061 attgtcacag gaaacttgaa caattcaaga gaatatttaa cttttggatt atctaagatg
     6121 caaggggtat gcatattatt acttggaaca gggactatcg caggaattat acagatgtct
     6181 gaattacaac agagtacaat aggtgcttta caatttttaa atatgccaca atttttattg
     6241 gcaccagttt cgggtgtact tatgtcatta gcaacagcat catcaacagc tggtgctact
     6301 atagcatcat ctacttttca tgatgctata ataaatggtg ggctatcacc gatttcaggt
     6361 gcatcaatag taaatgctgg gtctagtgta ttcgagcaat taccacatgg ctcactattt
     6421 catactagtg caggtagtat aaacatggat ataggtgaaa gatttaagtt aattccatat
     6481 gaagcattga taggaatagt aatgactata atttcaacat ctattcaatt agttttataa
     6541 taaatataaa tatttaatga aaaaagtagg attttgttat taaatattga atttattata
     6601 gtaaaaaaga agattggagt tttaaagtga ataaaaaaaa gattgttatt atagggatta
     6661 tttattcatt tttagtagta ttttcactta caaatatgta tgtaaatatg gagtataatc
     6721 taaatgtatt tgaatatatt aagaagtcac ttccatttac agaagaagaa aaaaagtggt
     6781 tagaaaaaca caagaatttg atttacagct ctgaccaaag ttcacctcct ttgagatata
     6841 aaggtaagga agatggacag tataagggaa ttattgttga tttaataaat tctctgtcaa
     6901 ttcaaattgg tagagatttt tattttaaac caaataattg gtggaaagaa tctttcgtaa
     6961 attcaataga tgatagtata aagttttttg acttgattcc atcaaaagaa agggcaaata
     7021 aatttatatt tactgaccca atatacactt tgagtgcaaa tatattgaaa gataaaaagt
     7081 cccaagatat aaatagttac atggatttaa aggggaaaac agtagcaatt ccagaaggtg
     7141 attattctat aaatttttta aagcaaaaaa ttcaagatat aaacattttg ttgactccag
     7201 atataaagac tggagtcaac catttaatgt ctggaaaagt agacgcagtt gttggtgatg
     7261 aaccagtatt gcgatactat ataaataatt atggactttc aaataaatac agtgttttaa
     7321 gtaatcctat atatactaaa aaagcagttc ttgctgtacc taaacaatat gaagagctgg
     7381 ttagtatttt aaataaaggc atttttaaac ttcagaaaaa tggagtgtat aaagatttaa
     7441 agaaaaaatg gtattctacg tataatgaag ttgatgatat attgtatgag cgaggaattg
     7501 tgcctagtat ttatcttttt ataggtataa ttttaatatc aatttatgtt ttttatagct
     7561 atacatattt gttaaagata gaaatcaaaa ggaaaacaga acaggtaata gaaaataaaa
     7621 aaactttaga ggcaactttt aatagtatta cagatataat aatgcttgta gatgaaaata
     7681 acaatatagt agaatcgaac aaagtactat atgactttat gggtgaaatg agttataaga
     7741 tagcagattt gattagcatg ataaaaggtg ttattgaaaa taccttctct gaaaatacta
     7801 ataaaactag tgaaatagaa atacacaata aaattttaaa gataaacact tttcctgtag
     7861 agtataaaaa aaataatact gagtatatag tagttcttat taaagatata actaatgata
     7921 aaatagtaga ggcgaaatta cttagagaaa acaaaatgat atctataggg caattagctt
     7981 ctggggtagc acatgagata agaaatcctt tgggtataat aagaaataat tgctatctct
     8041 taaaagataa cgttacaatg gaagaagtaa atgattgtgt taaatcaata gaaagtaatg
     8101 tagatagagc aagtaatata ataaccaact tgttaaattt cgcaagaata tcagatgata
     8161 atttagaaca tataaatata agaaatttta ttgaaaatat agtgaaactt caatataaaa
     8221 tgttacagtt aaagaatgtt gaaataaaaa ttgactgtga acataattta atatgctata
     8281 taaatggaga atcgttaaaa catgtattta taaatttaat ttctaattca atagatgcaa
     8341 tacatcaaga tggaaaaatt attatatact gttatgaaaa aaatcattgt ttatttatag
     8401 attttaaaga taatggtgaa ggaataaaag aagatgccct aaaggacata tttaatccct
     8461 tctatacaac aaaaccaatt ggtgagggaa ctggtttagg tttatatatt acttataatg
     8521 agattaaaaa gaataatgga gatataagtg ttgaaagtaa attgggggtt ggaacttgtt
     8581 ttcatataaa aattccatta aataaagagg tgactatata atgaaaatac tattagttga
     8641 tgatgagcta gaatatggag tggtaatgaa aaaaattcta caaaagaagg gctatcttgt
     8701 agatatagca ctaagtggtg aagaagctat aaatataatt aaaaaagata aaaattatga
     8761 tttggtttta tctgatgtta tgatgaaaaa tatggatgga gtacagctat tagatagaat
     8821 taaatctatt aatagagata tagaggttat attagttaca ggatatggaa gtattgaaaa
     8881 tgcagttgat gctatgaaaa gaggagcttt atcttatttt ataaaaagta atcccattga
     8941 aaatttatta gaagaagttg aaaaggtaaa ggcaagtaaa atatctgttt ctgtacaaaa
     9001 aaataatttg gaatttactt tggaatctaa aaatagagat tttaataata tcataaaaat
     9061 agcaaaaaaa gctgcttgta aagatgttaa tatattaatc ttgggggaat ctggtgtagg
     9121 aaaagatata ttagctagat atatacatag cataagtcca agaaagaatg agatatttgt
     9181 acctgtaaat tgttgttctt tttcagaaaa tctattggag tctgagttgt ttggtcatga
     9241 aaaaggttct tttacaggag cagtagatag tagaaaaggt agatttgaac tttcgaataa
     9301 aggaactcta tttttggatg aaataggaga tatacctcta aatgttcagg taaaactttt
     9361 gaggacactt gaggacaaat ctatagagag gataggaagt aataaatcta ttaaagttga
     9421 ctttaggtta atttgtgcta tgaataaaga accaaaagtg gaaatatcaa atggaaatat
     9481 aagagaagat tttttttata gaataagtac aataacaata actataccac cactaagaaa
     9541 gagaagagaa gacttaacta ctcttataga atttttctta aataagtacc aaattgaaca
     9601 tgataaaaag atacactcta ttgataagga agtaaaggat tttttattaa attacaatta
     9661 tccaggaaat ataagagaat taaaaaatat aataaataga cttgtagttt taagtgaaga
     9721 aggcaattta tcaaaagaca atttaaattt aatatcaaat aatgtataca tagatgataa
     9781 aataagtatt agacctttaa gagaaataag aaaagagttt gaatgtgagt atattgaaaa
     9841 agttttatct ttgtgtggaa ataatatttc aaatacagct aaaaaattgg agattagtag
     9901 aagacagtta accaataaaa tatcagagta taatattaag tgagaaaaaa acttctcatt
     9961 aagtgagaaa aaaatttctc aaactgtatt aaaagataaa cagaaaaaat ctgtttatct
    10021 ttttttatta taatttt
//
LOCUS       DAELJO010000146        10012 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELJO010000146 DAELJO010000000
VERSION     DAELJO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.146"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            82..2112
                     /locus_tag="KPB88_002869"
     CDS             82..2112
                     /locus_tag="KPB88_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055132.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAKKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            2109..2561
                     /locus_tag="KPB88_002870"
     CDS             2109..2561
                     /locus_tag="KPB88_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055133.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            2563..3618
                     /locus_tag="KPB88_002871"
     CDS             2563..3618
                     /locus_tag="KPB88_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG0055134.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            3685..3993
                     /locus_tag="KPB88_002872"
     CDS             3685..3993
                     /locus_tag="KPB88_002872"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG0055135.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            4097..5362
                     /locus_tag="KPB88_002873"
     CDS             4097..5362
                     /locus_tag="KPB88_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG0055136.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            5450..6166
                     /locus_tag="KPB88_002874"
     CDS             5450..6166
                     /locus_tag="KPB88_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0055137.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENAMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(6361..6654)
                     /locus_tag="KPB88_002875"
     CDS             complement(6361..6654)
                     /locus_tag="KPB88_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG0055138.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(6660..6875)
                     /locus_tag="KPB88_002876"
     CDS             complement(6660..6875)
                     /locus_tag="KPB88_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055139.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            7322..7720
                     /gene="tnpA"
                     /locus_tag="KPB88_002877"
     CDS             7322..7720
                     /gene="tnpA"
                     /locus_tag="KPB88_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0055140.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            7717..8880
                     /locus_tag="KPB88_002878"
     CDS             7717..8880
                     /locus_tag="KPB88_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0055141.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(9264..9776)
                     /locus_tag="KPB88_002879"
     CDS             complement(9264..9776)
                     /locus_tag="KPB88_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0055142.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT         3839 a         1075 c         1732 g         3366 t
ORIGIN      
        1 cttagataaa aactgaaaca tacataataa tataaaattt gctatattaa taactctaat
       61 tattttggaa ggagggaaaa tatgataagt gggagaatgt tactcatcat gaattttcta
      121 aaaggaaaaa ataagacaag ttacaaggat attagcaaac aacttagtat agaagaaaga
      181 aaagttagat atgatataaa taatttaaat attgttttgc gttcattaaa caagtcagta
      241 atacaaaaga tgaataaagg tgtgttaatt attcctgatg acttccaagt agtatgtata
      301 aataataacg aatatgtatt ttccttaact gagcgagtat ctattatgaa aattatatca
      361 atgtttaaaa tagataatct aaacttagaa aaattaagta aagaatttca ggtttctaga
      421 agttcaatta aaaatgacct taatgttttg tcaagtgagt tagaaagaaa ccaaataact
      481 attacttatg ataaatcttt taaaataagt ggagaagaag acattatata taagcaaaga
      541 ttgaacatat taaaatcgta ttcttatctt tttggtaagg aaaaatctga tttaagtgta
      601 tttgagaagt atttagtgaa tgtaattgaa aaaatattca aagggattta tttgattgat
      661 atatataatt ggtctatgaa tttattattg caaatgggat ggactttaaa tgatgaatct
      721 tttacatggt atgtgtcaaa cattttcctt tttacatggt atatacattc agatgaaaaa
      781 catcctttag aatctgcttc attaagtgag ccgtgttttg aaaataagtt tgaccaagaa
      841 tttgaaacaa ttattcaaaa aaagttgact aaggaacaaa tttttctgtt aaagagtttt
      901 gtatttttta caaataaata tgcaagttta aacgaggata tggatttgat tagtacagaa
      961 attatagttc aagacctaat ttacaatatg tcagaaatat tagagattag ctttaaagaa
     1021 gatatgattc tttataaagg attactcaat catattgccc cattgataga gagaatcaaa
     1081 cagaatatac agatttatga ggaattactt catgtaatac cagataaata tcaatatgta
     1141 cttgaagcta ctaaggtttc tattcaaaaa aacagagttt taagtcaaat aaagaatgaa
     1201 aatgaaacaa tacttttggc aatacatttc ttaggaagta tacatagaaa tgagaggaat
     1261 aactacaaaa atatattgtt agtttgtgga tttggatatg gtgtaattgc tatgttaaag
     1321 gatagtttag taaatgatta ccagataaat atagtagaga gtattccatc atataagtta
     1381 caagattata aagaatggga aagtattgac ttcattataa caacatcaaa gatagtaata
     1441 gacttgccta aaacaattat tgagataaat ccatttttac aagaagagga ttatttgaag
     1501 tttgaagaaa aagggataaa aagaaagaac gtcttaacta attatatgtc aataaaaaaa
     1561 aggttggact ttttagaaga agatgccaaa aaaagagtat taagtattat atgtcaagaa
     1621 cttggataca gtgatgagag aatattattt agacaactta atttaagtga tttaataggg
     1681 attgatacca taaggataat tgatgaagat atgaaatggg aagatgctgt aaaaattagt
     1741 tcaaacatac ttgctaattg tgattttgta tcagcacaat atgctgagaa tattatagat
     1801 attttaaaaa atgtgggttt ttatgctgta aaagataacg aatttgcttt actacatggc
     1861 aatgatagtt cattagtcaa agtaagttcg ataagtttat taatatgtaa agaagcagtt
     1921 agttttggag ataaaaaggt taaaatcata ttttgcctag caagcagaga taaaaaagag
     1981 caaattcctg caataattaa tctaactaaa atggtttata agactaattt tattgctatg
     2041 ttagaaagtg caaagacaaa agaagaagca gaagttttaa ttcataaata tgagaaggag
     2101 gtcactgtat gaataaatta gttaatgtaa aatgttcatt taaaatacat gttaattcaa
     2161 aagaagaagc cattatatcg ttagttgatg tagtgtcaaa agaaggatat ttaatagata
     2221 aaaatcaatt tttaaaagat gttttaaaga gagaagaaac attgtcgact tatattggac
     2281 atggtatagg tcttcctcat agtcaaagtg taggtgttaa aaactcttgt attacaatag
     2341 gaaagcttga tactcctatt gaatggacag aagaaggaga aaaagtggat ttaatatttt
     2401 tgatatcagt aacaaaagac aatgaaaata atcttcattt aaagatttta tcaaaattag
     2461 cacgtttatt gatgcatgaa agttttagaa atcaaattag atgttcagat gaacaaactg
     2521 tttataatct tataaaagaa aaaatagagg aggaagatta aaatgagaat tttaaagttt
     2581 ttgcaaaaac atcttatgac agcaacatct tatatgattc catttgtggt tgcaggagga
     2641 atattatttg ctttatcagt aactctaagt ggacaagctg ctgttcctga aacaggctgg
     2701 ttggctaaac tgaatcaaat tggagctgct ggcttggctt tattcattcc tatattaggt
     2761 ggatatatag catttagtat ggcagataaa ccaggattag ctccaggtat gataggtgca
     2821 tatttagcaa aagaagtaaa tgcaggtttt ataggtggta tcatagcagg atttatagca
     2881 ggatttgttg ttttacaatt aaagaaaatt aaattagctc ctacaatgag aactttaggt
     2941 tctatattta tttatccttt gattggtaca ttgattacag gtggtataat tgtattttta
     3001 attggagaac ctatagcttc ttttatgaca tggatgacaa actggttaaa tggaatgtct
     3061 ggtgtttcta aaattccttt aggtggaatc ttaggaggaa tgatagcctc agatatgggt
     3121 ggtcctataa ataaggttgc tgctactttt gcccaaactc aagttgacac acttccatat
     3181 ttaatgggtg gagttggcgt tgcaatttgt atacctccaa ttggtttagg attagctaca
     3241 ttattgtttc caaaaaaatt tagcaaggaa gaaagagatt caggtaaagc ttcactatta
     3301 atgggatgtg ttggaattac agaaggagct attccatttg caacatcaga tccagttaga
     3361 gtaatacctt gtattatggc tgggtcgatt gtaggaaata ttatggcttt tttattagga
     3421 tgtttaaatc atgcaccctg gggaggcctg atagtattac ctgtagttga caataggttg
     3481 gggtatattg catcagtatt aactggagca gtagttgttg cagtattaat gaaattagta
     3541 aaaaaagacg tgaaagaaga tgaagaaatt gaagaggatt tagatgactc aattgaatta
     3601 atatttgaag aattataaat ataaatggaa ggaatttgta taaaaaagta aatataaaac
     3661 tataaattta aaggagataa gaatatgaaa attgtagcag taacatcttg tcctagtgga
     3721 gtagcacata cttacatgtc ggctgaatca cttgaattat cagccaaaaa atttggtata
     3781 gaaataaagg ttgagactca aggaagctct ggaatagata atgaattgtc acttaaagat
     3841 attgatgaag caacttgtgt aatattgact aatgatgttg agattagaaa tatggaacgt
     3901 tttaagggaa agaaggtagt aagaatgagt gtatctgaga taattaagaa atctgatgca
     3961 ttaattaaga aaataaaaga tacttttcaa taaaaattga tttattttac atataactca
     4021 aagatgtttt taaagaaatt tttattaaaa gcatctttga aaatatctta aaaatagaat
     4081 aaaaggagat atagtcatga aaaagttacc aataaagact gtcgtacaaa gattactaga
     4141 cctacaagag gaggggaaaa gtgcaactct tttaggaatt ggtcctatgt caccaaactt
     4201 gttacaagca agttttgaac tagcaaaaga tgatgatttt cctttaatgt ttatcgcaag
     4261 tagaaatcaa gtagatgcag atgaactagg tggcggttat gtaaatggtt ggaatcagga
     4321 aacttttaca aaagatatta agaagattgc cgacaaagtt ggatttgatg gtttatatta
     4381 tttatgtaga gaccatggtg ggccatggca aagagataaa gagagaaatg accatttgcc
     4441 tgttgatgaa gcaatggagt taggaaaaaa atcttattta gcagacattg aagcaggttt
     4501 tgacctattg atgattgacc ctacaaaaga cccatttgaa attggaaaag taattccact
     4561 agatgtagta ctagaaagaa ctgtagaatt aattgagtat tgtgaaaacg agagaaaaag
     4621 acttaatcta ccagacatag gttatgaggt tggtacagag gaaaccaatg gaggtctgac
     4681 ttcaactgag acgtatgaaa catttataac tagactaaag gttgaacttg ataatagagg
     4741 tttgccaatg ccaacattta tagttggtca aactggtact ttaactagaa agacagaaca
     4801 agtaggaact tttaatttta gaaatgctta tgatttagct caaatggcta agaaatatgg
     4861 agttggttta aaagagcata atggcgatta cttagatgat gtaactttat tagaacatat
     4921 accatctcaa attattgcta caaatgtagc ccctcaatat ggtactgaag aaactagggc
     4981 atatttaaaa ttagcagaag ttgagtgtaa attagaaaaa gagggcttag ttgagaaaac
     5041 ttctaatatc agacatgttc ttcttgttaa tgcaattgaa tgtggacgtt ggagaaaatg
     5101 ggtagtagga gaacaaaaaa atctaactac tgaagagata tttaaggatg aagttttatc
     5161 taatgaaata ctagatattg caggacatta tacatttaat aatgatgatg ttaaaaaaga
     5221 aatagaagtg ctttatgata atttaagtaa gaataatatt gatggtcaaa gatttgttgt
     5281 agaccatatt aaacgtcctt tgcgtgatta tgcagaatgc tataacttaa aaggtgttac
     5341 tacgaggatt ttaaataaat aataggactt atagtggtga gtattgttat actaggtatt
     5401 tatagttata attatactta ccactattaa atttaggagg actaatattt tgattaattt
     5461 aatttttgat gtagatgata ctttatataa tcaattaacg cctttttaca ctgcatataa
     5521 taaagtgttt tcttcaataa aggatatttc tattgaagat ttatatatga gtagtagaaa
     5581 gtacagtgat gaggtatttc atatgactga gaatggagaa atgccaatca aagagatgca
     5641 tatttatagg ataatgaaag cttttgagga gttgggaaat tctattacag aaaaagatgc
     5701 acaaagtttt caagatgagt atatatatca gcaatcacaa attacactaa taccagaagt
     5761 tgagtgggtt ttaaattttt ctaaagaaag aaatattaat ctaggaatta taactaatgg
     5821 cccatcaacc catcaaagaa tgaagttaaa gcagttaaat attgaaaatt gggttgataa
     5881 aagtaatatt tttatttcta gtgaggttgg attttctaag ccagatacta atatttttag
     5941 agtagctgag aatgctatga atttagatag agaaaataca tattatgtgg gtgattctta
     6001 tagaaatgat gtattaggtg ctaaaaaagc tggttggaag agtatatggt tgaaccatag
     6061 gggacatgag ttagaagagt tgttttataa acctgatttt gtaattttgg aacataagga
     6121 tttgatatct ttatttataa aaatatgtag ttataaaaat atgtagtaaa aaaatgttaa
     6181 taatagaatt gtataaaata attaataata actatataaa attatatgtt tagattgtga
     6241 gcaatctatt ttatgttaaa aatagattat ttagaagaag gggaaatctt aaaaaaattt
     6301 gaatatcccc tttttattat ttaattaaaa tgaaatagtt agaaatatat tttatttagt
     6361 ttagtcaata acattttggg attctttata actacatgtt aactgttgaa taggtttatc
     6421 atatggttta acccatcctt tattaatact taattctgta agtgctgtgt gaccttctac
     6481 catttgagtt aatgaagctg aatatatagc tcttagttca ggtgtactac ttgtcaatgc
     6541 agaattaaga tacatattgg cagatgcttt agcagatgaa agcatactta acattattac
     6601 tttatcgtct atataagtat tgttcttaat tagattttct atcatatttc tcatgttatc
     6661 tatacctcct cagaaattag tatttcattt tcaactataa attgttgtat agctttaatt
     6721 cttccttctg ttgctaatat actagattct gattgtcttt ttagatcttc atctgatatt
     6781 aacatattta ttgctctttg cataagtaat ccgtcagact ccatttttat aattgctgct
     6841 aaagatattt tttcagtttc tgaaagtatt ctcatttaat catctcctat attgtaattt
     6901 atgctttaat agtatgtctt tcttttcaag gaaataacca ttacactttt ttacagtatt
     6961 ttatattctc gttatatcaa aatttagtcg acttaatata ttaatataat tttgtaggat
     7021 atttatcatt aaatatctat aaaataaatt gtttgaatat tcataaagca aaatttattt
     7081 gtatgtactt cttcaagagt aaatatgagt tttttcaaaa aattaatttt tatgataatt
     7141 attaagggct atatttttct taaagttgtg attatcatta ttaatttaca ataaaattgc
     7201 cgaaaagccc tctcctttag gtgtagggat gaaaggtaag gtcttaatat aaaaaagtta
     7261 gaataatgct tgtaatctaa taccgctatt gataccctat atttaggagg tgttagtttt
     7321 tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg tattttgccc
     7381 taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag aaatactacc
     7441 atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata aagaacatgt
     7501 gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa aaggtttaaa
     7561 aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta gtatgcctag
     7621 catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag aagtagttaa
     7681 acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca tagagtgaaa
     7741 ttaaacctaa ctagaacaca atttgaatta gtaagggaaa agcaaatgga aagtgctaac
     7801 tgttggaact acatagttaa cttatctaaa gaatactatt ttgaacataa acaatggatt
     7861 aaaaagaatg atatacaaaa gttaatcaaa ggtaagtaca atcttcatag tcaaactatt
     7921 caagctatat cagataaatt tgatgcaaat agaaagacta tatcagaatt aagaaaaaaa
     7981 ggtaatacta aagcaaaata tccatacaag actaagaaat tttatataat accttttaaa
     8041 gctagtgcta ttaatagaaa tgctaaaggc aacttaaaat tatcaatgtc aaaaggcagg
     8101 tatttagaat tagattttaa tgttgagaat ataaaaactg ctgaaatagt atggcgaaat
     8161 ggatattatt tatactacac ttttgacaat gaattaaatg gagttgtagc caaaggcact
     8221 aataccgtag gtgtagactt aggggaaata cattcgatag ctagtgtaac aaatgaaggt
     8281 gtagggctta ttctatctaa tagagaaggc agaagtatta aacaatttag aaataagatg
     8341 tatgcttata tatctaaaag attaagtaaa tgtaaaaaag gtagtagaca atcaaagaag
     8401 ttatggagat taaaaaataa gataagaagt aaaacagata atcagttaat gaatttatat
     8461 catcaaacaa ctagaaagtt tatagacttt tgtgtagagc agaaggtatg tgaaatagtt
     8521 cttggggata taaaaggggt tgaaaaagat actaagaaaa agaaaagatt aaacagagtt
     8581 aatagacaga aaatatctca aatggagtat ggtcgaataa aggactatat aaaatataaa
     8641 gcaaaagagc aaggtattga agttaagtta gttaaagaaa actacacttc tcaaacttgc
     8701 ccaaagtgtt ctaaaaaaca caaaccaact ggtagaactt atagttgtac ttgtggttat
     8761 gaaactcata gagatatagt tggtgcttgg aatattttaa ataaaaagca taaatatggt
     8821 ttagtagatt ttagaataaa tcataagcaa ccaataaatt taaaagtatc taccgtttga
     8881 gccatgtgtg ggcgaataac tttaaggtag tagtggcgaa gacaccgtcc gaagtagctt
     8941 ggctttgtaa ccacctctgt tgtactgttt aagtataaat aagtgtgtta gccaccaaga
     9001 atccccttgc tttagctatg gggagtgtgt caataaaata ctttaatgaa aaatagctaa
     9061 ctttaattgt aggatattct taatatattt gaaataaata aaaatattga aggatataat
     9121 attcaattgg tatataaata ataaatgata gttaaatttt accttagagg ctaattaggt
     9181 gtttagtcaa agttaatgcc tttagaatac aaatgtattc taaaggcatt tttactatgt
     9241 attttcattt atattactaa acattatcta tttttatata catttctatg tattttattt
     9301 tcatcaaatc ggtctataaa ctcatttttt tcttcactat atccaataga tataagtgca
     9361 taaggaacta attttaatgg taagtctaaa gtttgtctaa ctttatttac cactttattt
     9421 ttaggaaata ctcctgccca acaagcagct agaccttgat tagtaacttc taataataaa
     9481 ttttgtgtgc aagcacctaa atcttgtatc catttaccag gttttaagaa tttatctcta
     9541 tttccaagca ctactataca taatggtgca aattctatat gctttgctct atgctgtgac
     9601 tttgagagct ctttcagcag ttccttatca tctactataa taaattccca aggttgagca
     9661 ttttttgatg aaggtgcttg catagctgat tttagtattt cagttatttt atcttcttct
     9721 atatttttat ctatatatgt tcttattgaa cgtctatttt ttataacttc taacatacag
     9781 attctccttt aaataaaatt atttctattt atataatatc attactttaa attttagcta
     9841 ttacttatat aaagaataaa ctttgtgttg gttttatcta agtaaaagta acattcagaa
     9901 ctaaaaaaca agactataaa tccaaagtat taattaaaaa agaagtaata aaaaataata
     9961 tacaagattt aaaaaagagt attgggctta aaatttaatt agaaaaccaa tt
//
LOCUS       DAELJO010000147         9983 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELJO010000147 DAELJO010000000
VERSION     DAELJO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.147"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            88..1518
                     /locus_tag="KPB88_002880"
     CDS             88..1518
                     /locus_tag="KPB88_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0055143.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1621..2724
                     /locus_tag="KPB88_002881"
     CDS             1621..2724
                     /locus_tag="KPB88_002881"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0055144.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2739..2975
                     /locus_tag="KPB88_002882"
     CDS             2739..2975
                     /locus_tag="KPB88_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055145.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            2992..4041
                     /gene="vorB"
                     /locus_tag="KPB88_002883"
     CDS             2992..4041
                     /gene="vorB"
                     /locus_tag="KPB88_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0055146.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4043..4783
                     /locus_tag="KPB88_002884"
     CDS             4043..4783
                     /locus_tag="KPB88_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0055147.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4786..5325
                     /locus_tag="KPB88_002885"
     CDS             4786..5325
                     /locus_tag="KPB88_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0055148.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5449..7548
                     /locus_tag="KPB88_002886"
     CDS             5449..7548
                     /locus_tag="KPB88_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG0055149.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7815..8330)
                     /locus_tag="KPB88_002887"
     CDS             complement(7815..8330)
                     /locus_tag="KPB88_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG0055150.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8422..9633)
                     /locus_tag="KPB88_002888"
     CDS             complement(8422..9633)
                     /locus_tag="KPB88_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0055151.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTHMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3747 a         1230 c         1847 g         3159 t
ORIGIN      
        1 tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata ataaaaagta
       61 agaaaatttt ataagggagg ggtagatgtg agtagtcaag aaagcaaacc aaaaggaaat
      121 cttatatcaa attttagaga tgatatgaga gaccctaaga aaagagcaat atacacatat
      181 tcaagttcta taaacttagc gtacaatttt ataggttact ttgtatcaac tgttacaaat
      241 atttttttaa ctgatgtggc aaaacttggt gtaattatgg caggtggtat gcaaattata
      301 caatctttca taaaaattgc tgtagctcct ataacaggta cagtatttga caaacaacca
      361 ttcaaaaaag gaaagtatta tccatggctt agatatgcac cagcagcgtt agcattaaca
      421 tatggaatat ttttcatagt tccattaatg aaaataagtc cagcattact tttacctttg
      481 atgacagctt tattattaat agcatcttgt gctcagcaag tagttttcac tataatttat
      541 gctatttacc ctgtagttgc taagacacct agagaccgag ttataggctc aactactacg
      601 aatatattta aagaagttgg taaattctta gtaggttttt catatccgtt attattagtt
      661 ttatttgcag atgtattagg tggagaagga atgggttatt taggtaccta cttattcttt
      721 gcagcaggat gccttttagt attttggttt tcttcaacag agataataaa atctggagca
      781 gaagaagaag ttcttcaaaa agaagagaag ccaaagatta aagcaagtga aatggcaaaa
      841 gctttattca ctaatgtacc acttatgatg gcattttgtt tagaatttct tatatgtata
      901 agatctatag gattaggacc tttggctcca tattatttta aatatgtaat agaagatgaa
      961 cgtggattag cagtattttt atcaatcatg cctttggtaa gtgtagcatt catgtttttc
     1021 gcaccagtgt ttataaaaat ctgtagagag caaaaactag cttcaataat ttctttttct
     1081 atatgcgcat tatgccactt attggttgca tggactcctt gggggaaaac tactttagga
     1141 gtaactatgt tgctggcaat tggtggaggt ttttcaaatg tagtatcaat aataaatctt
     1201 aatttctttg ccggttcttg tgattatggg cattggaaat caggaaaaga cttacctggc
     1261 ttaagtatgt cactataccc agttgcaata caagtaggag tattacttgc aactactata
     1321 agaacagttt taatgaactc aatgggatat caagctgata tggtagtcac agaagcagtt
     1381 aaatcaggtt ttataaatat gatttcttat tctatggcaa tacctttaat aatagctgtt
     1441 gttatagcaa tactatatcc agtaagtgac aagaaattaa accaaataag agaagagtta
     1501 aatcaaagaa atgcttagtt aaaagaaagt taagtagatg gttaatttaa gctattaaag
     1561 taagtagacg caatagcgtt tatataaatg aaaatacata tacagagggg gcttttaaaa
     1621 atgatagaaa aattattgag aaataccgta aaagaattac atcaatatgt tccaggtgaa
     1681 ccaatagaaa aagttaagga aaaatatggt gtaaaagaaa taataaaatt agcttcaaat
     1741 gaaaatcctc taggaccatc accaaaagca atagaagcta tgattgaaat gttaaaacaa
     1801 ggtcaacttt atccagagcc agaagctaac gaacttagaa gaaaattagc tgaaaagtta
     1861 gatttaaaac cagaaaattt tattgttgct aatggtgcag ataacgttat cactctaata
     1921 ggtgaggctt ttataaatag aggagatgaa gttatatatt gtaacccaac atttccatca
     1981 tatagaactg caactataaa aaatgaaggg ataccagtag aagtaccttt aacagaagat
     2041 tataagtatg atttacaagg tatattagat aagattacag ataaaacaaa attaatatgt
     2101 gtatgtaacc ctaataatcc aacaggaaca attgtggatg ataaagagtt agaagaattt
     2161 ttaaagaaag tccctgaaaa tattataact atattggatg aagcatatat agaattttta
     2221 acagttccta attatgtgga tggacttaag tatgtaaggg aaaattataa cgttatagta
     2281 acaagaactt tctctaaaat atatggtctt gcagggctta gagtaggata tggtattgct
     2341 aaagatgaga taataagaac tttatttaca gtaaaagagc ctttctctgc aaatcgtgtt
     2401 gctataagtg gagcaacagc agcacttgat gatgaagagt ttataaaaga aacatatgaa
     2461 ttaaatagag ttggaatggc ttactttaaa gaagagttta caaaaatggg atttgatgtt
     2521 gtagattcac aatcaaattt cctttatgta gatatgaaaa cagatatacc taagttattt
     2581 gaagacttga aaaaaagagg gtttgtaata agaccaagtg ctacacatgc acgtgtaagt
     2641 attggaacta tggaagaaaa taagaaattt gtagcagttt taaaggaaat attaaatatt
     2701 aaaggagagc caagtattgg ataatagata gaaatattat gaaacggggt gtagatatga
     2761 acttaaaaat aaacgcagag agatgtaaaa gttgtgaata ctgcgttata tcttgtaaaa
     2821 aaggagcatt aaaaatatca agtaagataa ataaagaagg ttatgctcat gtagaagttg
     2881 atgagagtaa atgcatactt tgtggaattt gctatcaggt gtgtccagat aatgtttttg
     2941 aaataattaa agaagaagca tgtcaagagg cttagaagga gttgggataa tatgaaaaag
     3001 atgtggaagg ggaaccacgc cattgcagaa gcagccctaa gaggaggttg tgaattttat
     3061 gcaggatatc caattacacc acaaacagag gtaatggaat ttttatcaca tagaatgtct
     3121 gaacttggaa gaacttttat acagtccgaa aatgagatgg ctgctatata tatggtatat
     3181 ggagcatatg caagtggaat gaggtctatg acgagttctt caggaccagg aatatcttta
     3241 aaacaagaag ggatttctta cttatgtgca aatcattacc cttgtgtaat cgtaaatgta
     3301 cagagatggg ggcctggtct tggctcattg gactcagcac agacagacta tctaagagat
     3361 actagagggg gaggaaatgg agattatcat ttgatagttt atgcaccaaa cagtattcaa
     3421 gagactgtag atttgatgta taactcattt gatgtagcgg agaagtacag agtgcctgtc
     3481 gaaattttga cagaagcagc tttaggtcaa atgatggaac cagtagaatt tccagagttt
     3541 aagaagagag aaggagattt aggttggact tatgatggaa gtaatagaga ccatgcaaag
     3601 gttgcagata accaaaagcc aactttttgt atggagaaac gtatgagaat aactgaaaat
     3661 gaacaacaat gggaagatta tcaagtagaa gatgcagaat atgtgtttgt agcgttcggt
     3721 ataccatcaa gaacaactat gaatgctgtt agaagactta gagaacaagg tgaaaaagtt
     3781 ggaataataa gaccaataac agtatggcct ttcccatata aagcatttga aaaggtaagt
     3841 aaagatgtaa aaggatttat ctcagtagaa tcaacagaca caggtcaatt agtagaagat
     3901 gttgctttag cctcaaagaa agtatgtaaa gaaaatgtac ctgtgtatgg tttatttagt
     3961 ggaaatcata taccaaaaac attgcaagta atggacactt atagcaaaat aaaatctggc
     4021 gaaatcaagg aggtgttcta atatggctac agaaaaatta acacctagcg ttgtttcaat
     4081 accaaacaag ttttgcccag gttgtggtca tggaatagta aatcgtataa tagcagaggt
     4141 aatagaagaa catggatatg aaaaaaatca tgtattaaca ttaggagttg gttgtgtctg
     4201 caatatgaat tttagctgga atggtgataa gatgcaaact gcacatggta gagcttcatc
     4261 tacagctata ggagttaaag tagcacttcc agatacatta gtaatgactt accaaggtga
     4321 tggagatgct tatgttatag gtctttctga aactttaaat acagcatata gaaatcataa
     4381 tgtgactgta tttgtaataa acaataataa ttttgcaatg actggtggac aaatgtcatg
     4441 gaccactatg ccgggtcaag tcacaacaac aagtgtaaat ggaagagata taaagactac
     4501 tggtagtcca ctgaaagttc ctgagatggt ggcaaacttc tttgatgtag catatgtagc
     4561 aagaggttca gttcatagcc caaaggaaat aattaatcta aaaaaatata taaaaaatgc
     4621 aatagaagct caattaaatg gagagggata ttccttagtt gaaatattag caccttgccc
     4681 aactaactgg ggtgtatcac tagagaaatc tatcaaatgg atggaagaag aaatagtgcc
     4741 atattatgct cttggcgaat ttaagcaaag ggatggtgaa tagttatgga aaaagagcta
     4801 gtatttgcag ggtttggtgg tcaaggagtt ttaactcttg gtctgattgt agctgagtca
     4861 gcacttgaat tggggaaaca agtaacttgg atgcctgcat atggtccaac tatgagaggt
     4921 ggaaaagcct atagtgtagt aaagttttct gatgagagta taggtgggcc tgatatggaa
     4981 gaaatagatg ttttagtagc aatgaataaa ccatctttag attatataaa tttagttaaa
     5041 gaaggtggaa cagtagttat taatacatct gcaatagatg agaatgtaac attaagagaa
     5101 gatataagtg tagttaaaat aaattgtcag gaattagctc aaaaagtaaa caatccaaaa
     5161 gcagcaaata tagtagtact tggagcatta attgctaaaa ctgggttatt tgaaaaagaa
     5221 ttagctttaa aaactatgtg tgatttcttt gaggaaaaag gaaaaggaaa gtttaatgtt
     5281 caaaatgaag cagcattcat ggaaggctat aataacgcca tttgataaat tacccttatc
     5341 ttaagtgatt tatctcaaga tttaataatc tttagtgcca agagaaactt catcttgaga
     5401 taaaattcac ttaaaatcac aactataaac atataggagc gtgaatttat gagtttggaa
     5461 aaacttatta gaccaaaaag tattgctata gtaggagtga ctgataaact tggttttgga
     5521 agaagtgcag cacttagtat agtaaaaagt aaggaaacag atagagtata ttatgttaat
     5581 cccaaaagag aagaattgtt tgggagaaaa tgttataaga cgatacaaga agttccagaa
     5641 gtagtagact gtgtagtggt atgtacgcct agaaatgtag ttccatctgt attaaaagat
     5701 tctggagagt taggaacaaa ggcagcagtg gtatatgcta gtggatttgc agaggaaggt
     5761 acagaggaag gtacagattt agaaaatcaa cttatagaaa tatcaaatac atacgatatg
     5821 aaaatattgg gtccaaactg catggggttg ctaaattgta tagataaggt taatctatgg
     5881 gcaggaggtt ctaaatggga tttagatact aaaaaacctg gtataggaat tgtaggtcaa
     5941 agtggattta taactgctga aattgtttca agtgattatt ttaatatatc ttatggtttt
     6001 tcaacaggaa atggaaatat agttacacta gaagatgcag tagatttcct tgtagatgat
     6061 gattatgcat cagtaatagc aatttacctt gaaggtttaa aaaatcctca aaaatttata
     6121 gatgctttaa aaagggcagc acaaaaaaga aagccagtaa taatattgaa atcaggaagg
     6181 tcagaaaaag gtgctatttc tgctgcttct catacaggaa atttagctgg ttcgagtaaa
     6241 gcttttgaaa gtatttttga aaaatatgga gtaatttctg tggaaaattt agaacagttt
     6301 atgtgtttag cacaagcttt tagtgtttta gatggtaatt tacctgcaaa ttctaatttt
     6361 gcagcaataa acttctcagg aggagaaaat actatttgtg cagatttagc agaagaaaat
     6421 ggtgtagaac ttgcagaaat atcttctgaa actaaagaag aaatgaaaaa atatcttcca
     6481 ggatttgcaa cacctaaaaa tccactagat gctacaacag cattattcca tgaaaaggat
     6541 atgatagtag gtttattaca tacctttgaa aaggatgata gtgttggatt tactatggta
     6601 ggtgcaaata taagagatga agaaaatgaa atgcatgaaa ccctttgtaa agctgtaagt
     6661 gaagctagag aacaaggatt gaaaaaacct gtgtttgcag ttcctacact tgaaggaaat
     6721 agatatttag attataggac tagattagaa gataatcaag tgcctataat gtcatcagtt
     6781 ggaactacat ttacttgttt taataaaatg gctaagttta tagattatga ttattcaaaa
     6841 agaactttag aatttaaagc tgttaagaaa agagagtcaa ataatgtagt agcattatca
     6901 gaattagata gtaagattga gatgaaaaaa tatggagtac cagttccagg acaaggtaat
     6961 gcaaaaagta ttgatgaatt agatgaaatc ttaaaatata ttaagtatcc tgttgtttta
     7021 aaaataaatt cttctgaaat attgcacaaa tctgatgttg gaggagttaa aataggaatt
     7081 aagaatagag atgaggcagt agatgcatat aatgagattt taactaatgt aaaaaaagca
     7141 aaaccagatg ctaatattga tggtatatta gttcaagaaa tggtagaaag tggtatagaa
     7201 attattattg gtataactaa tgatgaccaa tttggaccaa tgttattagt cggtttagga
     7261 ggagtatttg tagaggtatt taaagatact acattatatc cattacctat aaatcatgat
     7321 gaagctatta tgatgcttaa aaaactaaaa tcatttaagc ttttaaatgg ctatagagga
     7381 agtgagcctt gcgatattga tgctcttgca gatatgatgg tcaaattagg aaaatatgct
     7441 tatgaaaata aggatgaagt caaggaaata gacttaaatc cagtgtttgt atatcctaaa
     7501 ggaaaaggcg tatgtgcagt agatgcctta atagttaaat ataaatagtt tataagtttt
     7561 ggtattcaac cataattaaa gcaccgactg aaaatcacat tttgtaagtt agatagtgtg
     7621 attttcagtc accccaaatt taatttaaaa atattttaaa atgaaagtat agttttaagt
     7681 ttcattgtga aatagcactg tataaacaat aaaatactac gtataaaacg aataataaaa
     7741 ggtgttttaa tgataagaat caatcttgat tttcattaaa acaccttctt tattatatta
     7801 aaaattttta aacgttatat tgtcacaggt gggtttgttg gagctgcata tgtacgatta
     7861 gcaaattctt gatcaacaaa gaatagacca ccctctccag caagtttaac acgttttata
     7921 ttgtcttcac aatttgtttc ttcttctgct tgttcagata taaaccaaag taagaattga
     7981 gaagttctaa agtctgattc agagtttgct acagctgcta agttgtttat taaagctgta
     8041 accttttttt catgagctaa cgtacgctca agaatatcca ttgcagaagt aaagtctgaa
     8101 tctggcattg ggatagcatc aagctgaacc tttcctccta cattatgtaa gtatttgtaa
     8161 aaaaacatag cgtggtctgt ctcttcttta tattgaacat aaaaccaatt agaaaatcct
     8221 ttaagtcctt ctgcttcaag gtaagaagac atagaaagat aaagatacgc agaatataat
     8281 tcatggttaa tctgctcatt aagtaatttt tccattttag ctgaaatcat ttttatacct
     8341 ccaaatttta aatgctctta taaaatataa taatattaaa tggaaaaata ttcaataaaa
     8401 gtgtttaaat attttacata tttaataaaa atttatacta ccattaaaaa aattataact
     8461 tacaatggct atagagccaa ttaatggtgc atttgctcca aaatttgaat gccttacact
     8521 tattttttta tatttagaaa aacttgctga agcagtcaat ttttttaata atgtattttc
     8581 tataaatgta ccaggtatat taaagtcata gcctattatt atagaactac aatcaatcaa
     8641 tgtaagtgta ttaactaatg catatgatac gtatgtacaa aattcatcaa gcaaaaacat
     8701 ggcaagacta tctttattat ttgcctcatc tattaacttg actaaagaaa aatctttaca
     8761 gttaattaat ggggagttag gataaatatg gctcaatgat tcaattcttt taattagatt
     8821 actgacactt gtataataat ctaaacatcc attgttacca caactgcaca atggtccact
     8881 aaaattaatt gatgtatgtc ctatttctcc actttgtcct aggttgccag tatgaagttt
     8941 attttctaga actagaccag ctccaatacc attcattatg tgtaggtata tataatttga
     9001 tatattttta cctaaaccat acattttttc tgctaatgca cccgaattag catcatttat
     9061 caaaaatatt ggcaaatcag acatttcttt tataaaatct actatattta ggtttgatac
     9121 atttccaaaa tctgggggat ttagtatggt accatttata tcatctactg gacctattga
     9181 agaaatacca attccaatta tatctctctt gttgattttc attattttag taaacattct
     9241 ttttattata tcttttaaaa catcatttga tagacattta gggtaagttt cagaaagttg
     9301 ttttacaata ttacctttta aatctgaaat aattacctta catatatttc ttcttattag
     9361 gattccacaa atacaaggcg atatattaga aatatctaat aaaataggtt tacgacctga
     9421 tgaatgtgca tttaaagttg atgactctat ttccctaatc atattttcat taataagttc
     9481 agtaactaga tttgataaag tcattttagt aagtcctgta gacttagcta aatcaactct
     9541 ggacatatgt gtatttgtag aaagtaattt caatattatt aacttatttt taatttttac
     9601 atctttgatg ttagaacctt gtttgttact catcataaat ctccttaaat aaagttaagt
     9661 gttttattta ttttcaagta tactatcacc ttataataga gtaacatttt tttacttgaa
     9721 aatatcatta attagaatat atcacattat atatcataaa acatataaaa aaatgttaga
     9781 attaaattaa aagtgaaaag tagattgata tataaatagt aaatacttaa ttaaagaaag
     9841 ataatgatag caataattat tgttaccaag aatctactac ttcaagtgtt gccttaggta
     9901 tttaagttga tagtggtttg cacgtaataa acttgatgta acatatattc ttaataatat
     9961 cgataaaaga tttacttaaa aat
//
LOCUS       DAELJO010000148         9975 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELJO010000148 DAELJO010000000
VERSION     DAELJO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.148"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1020
                     /locus_tag="KPB88_002889"
     CDS             <1..1020
                     /locus_tag="KPB88_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0055152.1"
                     /translation="KKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEYIENME
                     SVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALEYVKE
                     QTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRRICLEA
                     IKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQYVNKQ
                     TDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDICIEI
                     VRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEYVKIA
                     IDDCI"
     gene            1159..1611
                     /gene="tsaE"
                     /locus_tag="KPB88_002890"
     CDS             1159..1611
                     /gene="tsaE"
                     /locus_tag="KPB88_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG0055153.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1611..2327
                     /gene="tsaB"
                     /locus_tag="KPB88_002891"
     CDS             1611..2327
                     /gene="tsaB"
                     /locus_tag="KPB88_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG0055154.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEIIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2317..2793
                     /gene="rimI"
                     /locus_tag="KPB88_002892"
     CDS             2317..2793
                     /gene="rimI"
                     /locus_tag="KPB88_002892"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG0055155.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2799..3815
                     /gene="tsaD"
                     /locus_tag="KPB88_002893"
     CDS             2799..3815
                     /gene="tsaD"
                     /locus_tag="KPB88_002893"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG0055156.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4112..6820
                     /gene="hpdB"
                     /locus_tag="KPB88_002894"
     CDS             4112..6820
                     /gene="hpdB"
                     /locus_tag="KPB88_002894"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG0055157.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6824..7081
                     /gene="hpdC"
                     /locus_tag="KPB88_002895"
     CDS             6824..7081
                     /gene="hpdC"
                     /locus_tag="KPB88_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG0055158.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7074..8024
                     /gene="hpdA"
                     /locus_tag="KPB88_002896"
     CDS             7074..8024
                     /gene="hpdA"
                     /locus_tag="KPB88_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG0055159.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8340..9278
                     /locus_tag="KPB88_002897"
     CDS             8340..9278
                     /locus_tag="KPB88_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0055160.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(9400..>9975)
                     /locus_tag="KPB88_002898"
     CDS             complement(9400..>9975)
                     /locus_tag="KPB88_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0055161.1"
                     /translation="KNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGII
                     KFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFT
                     LYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKEST
                     ITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3909 a         1040 c         1851 g         3175 t
ORIGIN      
        1 aaaaaattat ttttagatga taaagggtat tattttaata gtaattggaa agatattcat
       61 attggtattt atgatacaaa attaggagat tctttaaagt atatcactaa tgaatatatt
      121 gaaaatatgg aaagtgtaaa atacgattat cgtgaattaa aaaatataga aaatcaaact
      181 gatggtatgt gtattttagc aataaggcag aattacaagg cattagagta tgtaaaaaat
      241 caaaccgaag aattatgttt agaagcaata aagtataatt ataaagcact tgaatatgta
      301 aaagaacaaa cagaatactt gtgtcttgaa gctataaaaa aagattgtaa tgcacttaag
      361 tatgtacgaa ataaaacaga agggcttatt ataaaagcaa taagtcattc ttccaatata
      421 gatgttgtaa gtatactaaa ggctttagag actcaaacaa gaaggatatg tttagaagca
      481 attaaaaaag atggcaggtg tcttgcatac gttagagaac agagtgaaga attatgtata
      541 gaggccatta agcaaaatta taaagcatta aaatatgtaa aaaatcaaac ggaaaaaatg
      601 tgtatagaat ccgtaagaca aaatggtatg gcattacaat atgtaaataa acaaactgat
      661 aaaatatgta tagaagctgt caaacaagat ggaagaagta tacaatttgt caacaataag
      721 acagaagaaa tatgtataaa tgctataaga tatttaaata aaaaatataa tattaaagat
      781 gtactaagtt acatagataa gtatacagaa gatatatgta tagagattgt aagacaaaat
      841 ggaaaaatgc ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga agcagtaaaa
      901 gagaactata agtctcttaa gtatgtaaaa gagcaatctg agagaatatg taaagaagca
      961 ttgaagcaaa accataaagc aaaagagtat gtaaaaattg ctattgatga ttgtatataa
     1021 ggtgtagata cagccatcaa tagtgaaaga taaaaaaaga taaagacaag tactttataa
     1081 taatctaggc ttatactata attaattaag cagtgatata aattcacatg acgattatat
     1141 caaaatggag atgaattgat ggcaaaaata tatttagaaa atgaaaataa aactagagaa
     1201 ataggatata aattaggtaa acttttaaaa gaaggcagtg ttatctgttt agtaggagat
     1261 ttaggagctg gtaaaactac aatgacacaa agtttggcag attcattagg gatagaagat
     1321 tatataacaa gtcctacttt tactataata aatgaatatg aaggaaaaat acctttgtat
     1381 cattttgatg tttatagaat aggtagtagt gatgaaatgt atgatattgg ttatgatgaa
     1441 tatgtaaatt caaatggaat ctgtataata gaatgggcta atctaataga agatatactt
     1501 ccaaaagaat atttgaatat agaattgaga tataaagatg aaggaagaga aatgatatta
     1561 actccaaaag gagagtttta taaagagata gttgaggagt tgattaaata atgaaaatac
     1621 taggtatgga tacgtcttct atggcagcaa gtgtagcagt tgttgaagat gataacttaa
     1681 tatgtgagtt tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg ccaatgatag
     1741 aaaatatgtt gagtatgagt gatttaagta taaaagatat ggatttatta gctgtatgta
     1801 taggtccagg ttcgtttaca ggacttagaa taggtatggc tacagtaaaa gctatggccc
     1861 atgtaaataa tataccaata atagcagtaa attctttgga atctctagca aataacataa
     1921 atttctgtga tagaaagata tgttgtatat tagatgcaca gaaaaatcaa gtttattcag
     1981 ctaaatacaa gtttgaaaat ggagagataa tagaattaga tggtgttgat gttgtagagt
     2041 ttgatgcttt agtagatgaa attgtatcaa ctaatgaaga atttataata gttggagaag
     2101 ctgtatacaa atataaagac aagttagaga atataaaaaa tataaaaata ccttcaccag
     2161 ctaataatgt atctaacgca ggaagcttat gctctttagc attaaataaa tataataaaa
     2221 atatagatgt acatacttgt tatacgataa atcctatgta tatcagaaaa tctcaagctg
     2281 aagtacaata tgatgaaaaa atgaagaggc taaataatgg aagataatac aaaactaata
     2341 gtagatgata taaaaataga agaaatgact acagaagata tagatgaagt ttttgaagta
     2401 gaaaaaaatt gttttgaaga ttattggtca aaagagtctt ttagaaaaga gttgtctaat
     2461 gaagtagcaa ggtatatagt agctaagtta aatggaaaaa ttgtcggata tgtgggaatt
     2521 tggcttatac ttgatgaagg gcatataaat aatgtggctg ttcatagtga ctatagaggg
     2581 aaaaaaatag gggacaagtt gataaaaggc atagttgatt tatgtaaaga taataatatt
     2641 gcatctatga cattagaagt tagagcatca aataaaatag cacaaaattt atatagaaaa
     2701 tatggattta aaatgggtgg aataagaaaa gaatactaca gtgataataa agaagatgca
     2761 attataatgt ggaaccaatt aaaagaggtg tagagtttat gagtgatata ataacattag
     2821 ctatagagag tagttgtgac gaaacagctg catcagtttt aaaaaatggt agagaagtac
     2881 tttcaaacat aatctctact caaatcgaga cacataaaaa atttggtggt gtagttccag
     2941 aagtggcctc aagaaaacat gtggaaaata ttgatatagt tgttcaagaa gctttagaca
     3001 aagcaaatat aggttttaat gatatagacc atatcgcagt tacatatgga cctgggctag
     3061 taggtgcttt attggttggt ctatcttatg ctaaagcact tgcatatact ttgaatattc
     3121 cacttgtagg agtaaatcat atagaagggc atctaagtgc aaactatata gagcataagg
     3181 atttaaaacc tccttttata acattaatag tatctggagg tcatactcac ttggttgaag
     3241 taaaagatta tggcaagtat gaaattttag gaaaaacaag agatgatgca tctggagaag
     3301 catttgataa gatttcaaga gctatgaatt taggctatcc aggaggtcct attattgata
     3361 atctggccaa gaatggaaat aaacatgcta tagaatttcc aagagcctat ttagaagaag
     3421 atagctatga ttttagtttt agtggattaa aatcttctgt attaaattat ctaaatggaa
     3481 aaagaatgaa aaatgaagaa atagtagttg aagatgtagc agcatctttc caagaggctg
     3541 tagtagaggt tttatctact aaagcactaa aagcagtaaa agacaaagga tacaatatca
     3601 taactctttc tggtggggtt gcttctaatt cggggcttag agctaaaata acagagcttg
     3661 ctaaagacaa tggtattact gttaagtatc ctcctctaat tttatgcaca gataatgcag
     3721 ctatgatagg gtgtgcagga tattataatt ttataaatgg gaaaacgcat gatatgagtt
     3781 taaatgcagt tccaaattta aagataaatc agtagagtta gatgaatttg taaatataaa
     3841 agtattgttt caaaaaagta agtagaagtg aaattaaata taataaaaat aaatatagta
     3901 tatattaaaa cttgaagtaa aactatttat aatctttata ttccaatgaa ttgcaacaaa
     3961 ttttaggata tacagattgt ctttggctta gatttgcttc aagtttattt ttttctatag
     4021 aaagattatt ttaaaaagtg ggtaatatac cctttttttt cattcttaaa aagttttata
     4081 ctaattatag aaagattaag gaggggaaga aatgagtcaa agtaaagaag acaaaataag
     4141 aagtattttg gaagcaaaaa atattaaatc aaattttcaa aataaagaaa atttaagtga
     4201 atttaatgaa aaaaaagcaa gtaaaagagc agaagactta ttagatgtat attataatac
     4261 attatctact gctgatatgg aatttccata ttggtataat agagaatata gaaaatcaga
     4321 tggtgatata ccagtagtta gaagagctaa agctcttaaa gctgcttttt cacatatgac
     4381 accaaatata atacctggtg agaaaatagt aatgcaaaag acacgccatt atagaggttc
     4441 ttttccaatg ccatgggtaa gtgaaagctt ctttgttgct caaggagaac aaatgagaga
     4501 agaggcaaag aaattagcca gtaacacagc tgatgaactt acaaaatttg ggtctggtgg
     4561 aggaaatgtt actgaaagtt ttggaaatgt cgtttcaata gctggtaaat tcggaatgag
     4621 aaaagaagaa gtacctgtac ttgtaaaaat ggcaaaggaa tgggttggaa agtctgttga
     4681 agatttaggg tttcactatg aaaaaatgat gccagattat gatttaaaag aaaacctaat
     4741 gagtacactt atatgcatgt ttgattcagg gtatacactt cctcaaggta gagaagttat
     4801 aaattatttt tatcctttaa attatggatt agatggaatt atagaaatgg caaaagaatg
     4861 taaaaaggca gttgcaggaa atgcatctgg agatgggctt attggtatgg accgtctata
     4921 tttttatgag gctgttattc aagttattga aggattacaa acttggattt taaattatgc
     4981 aaaacatgca aaatatttag aaagtataga gacagattta gaagctaaaa aagaatactc
     5041 agatttagtg gagatattag aacatattgc acataaacaa cctcgtacat ttagggaagc
     5101 tcttcagttg acttatacaa ttcatatagc atcagtaaat gaggatgcta tatctggtat
     5161 gtctatagga cgttttggtc aaattttata tccttggtat gaacaagata tagaaaaagg
     5221 actaattaca aaagaagagg ttatcgaatt attagagctt tatagaataa aaatcacttg
     5281 tattgactgt tttgcttcag cgggcgtaaa tggaggagta ctatcaggca atacatttaa
     5341 tactttatct ataggtggat taaaagaaga tggttctaca ggagctaatg aactagaaga
     5401 gttattatta gaagcaagta tgagatgtag aactcctcaa cctagtttaa ctatgctata
     5461 tgatgaaaag cttccagaag atttcttaat gaaagctgct gaatgtacaa agcttggttc
     5521 tggttatcca gcatgggtaa acaactcaaa tggaacaaca tttatgatga aacaatttgc
     5581 tgatgaaggt atgactgttg aagaagcaag agcatttgca ttaggtggat gtttggaaac
     5641 gtctccaggg tgttggaaac agttaacttt aaacggaaaa acatactcta ttgcaggtgg
     5701 agcaggtcaa tctgctggct ctggagttca ctttatagct aatccaaaaa ttttagaact
     5761 tgttcttatg aatggtaaag actatcgtat gaatatacaa gtatttgagc ctcataataa
     5821 gccattagat acttatgaag aagtcataga agtatttaaa gattattata aacaagcaat
     5881 aaatgtttta gaaagagcaa ataacattga gttagatata tggagaaaat ttgatacttc
     5941 tataataaat tcactactaa agccagactg tttagacaaa ggtcaacata taggaaatat
     6001 gggatatcgt tataatgcaa cattaaacgt agaaacttgt ggtactgtta ctatggtcaa
     6061 ttcatttgca gctcttaaga aacttgtata tgatgacaaa gcatttacaa tagaggaaat
     6121 gaaggatgcc atattaaata attttggatt taaagatgca ttagaagtag gaaattattc
     6181 tatggcagac caagtaaaag tagataagac tggtaaatat gatgctattt ataaggcctg
     6241 tcttgatgca cctaaatatg gaaataatga tttgtatgca gataatattc ttaaaaacta
     6301 tgaggtatgg ttatcaaaag tatgtgaaga agcacaatct ttatatgcta agaagatgta
     6361 cccttgccaa atatctgttt ctacacatgg gccacaggga gctgctacct tggctactcc
     6421 agatggtaga ctaagtggaa caacatattc agatggttca gtctctgcat atgcaggaac
     6481 agataaaaat ggagtatatg cattatttga atctgcaact atttgggacc aagcagtagt
     6541 tcaaaattct cagatgaatt taaaacttca tccaactact ataaaaggac aacaaggaac
     6601 taaaaaatta ttagatttaa caagaagtta tttaagaaaa ggtgggttcc atattcaata
     6661 taatgttgta gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca attatcgtca
     6721 attaatggta cgtgttgctg ggtttactca atattggtgt gaattaggta aaccaataca
     6781 agatgaagta attgctagaa cagagtatga aggggtgtaa aatatgagaa agcatagtga
     6841 ttgtatgaat ttttgtgcag tagatgcaac caaaggaatt tgtagattat caaaacaaat
     6901 gattaattta gatgatgcag catgtccaga gataaaagta atgccaaaat gtaaaaattg
     6961 taaaaatttt gttgaagcta atgatgaagg tataggcaaa tgtgttggcc tagagaaaga
     7021 agactgggta tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt ttaatgagta
     7081 gtcaaaagca attagaaggc atgatttttg atgtacaaag cttttcagtt catgatggtc
     7141 caggttgtag aacaactgta tttttaaatg gatgtccact gagttgtaaa tggtgtgcaa
     7201 atccagaaag ttggactgta agaccacata tgatgtttag tgaattatct tgtcaatatg
     7261 aaaatggatg tactgtatgt catggtaagt gtaaaaatgg tgcattgagc tttaatcttg
     7321 ataataagcc agttatagat tggaacatct gtaaggactg tgaaagtttt gaatgcgtca
     7381 actcatgtta ttataatgcg tttaaattat gtgcaaaacc ttacactgta gatgaacttg
     7441 tgcaggtaat taaacgtgat tctaacaatt ggagaagtaa tggaggagta acatttagtg
     7501 gaggtgagcc actacttcag catgaatttt tacatgaagt attattaaag tgtcatgagg
     7561 taaatataca cactgccatt gaaacgagtg catgtgtatc aaatgaagtt tttaataaaa
     7621 tatttaacga tattgatttt gcatttattg atataaagca tatggataga gaaaaacaca
     7681 aagaacaaac aggtgtgtat aatgatttaa tattagaaaa tatatcaaac cttgcaaatt
     7741 ctgattggaa tggaagatta gtacttagag taccagttat ctctgggttt aatgatagtg
     7801 ttgaaaatat aagtgatatt atatctttta tgcacaaaaa taatttggta gaaattaatc
     7861 tattaccatt tcacagatta ggagaatcaa aatggattca attaggcaag gaatatgagt
     7921 attctgataa gggtgatgtt gatgaaggtc atcttgaaga attacaagat atatttttgg
     7981 ataatggtat agcttgttat gttggtcatg agacagcttt ctaaaattaa atacaagttt
     8041 taattaaaaa aaggatgtta cactaaaagc tagagttaca tcctttttta tgtggttttt
     8101 atgtagaaat aattaagtaa agttttatat gttttatata ttgtttatat tgctttatgt
     8161 ccataaatat tgctttaaat atactttata tattttttat agtttaaatt acagttagag
     8221 aatatattac gcttacttca ttgaagcaga agttttgtgg taaaatacta ctatgaaaaa
     8281 atttaataga atatatttta gaaacaacta aaaataatag ttaggaggaa gacatctata
     8341 tgaacaaatc atggagcaac ttgattatta agaataaaga caaaaaagta cgttcagggt
     8401 ggattattat agctgttatg gctacctttt acatacttca gtattattta agcatgatac
     8461 ttgtggaaat aataagaaaa atcctggttg ctacaggtga cattaattta aaaacaaact
     8521 atttttcaga atatgctaat tggttaaatg atgttgggtt acagattgta tttcaaattc
     8581 taacagattc acttatgatt attattccta taattgtatg gagatttatt atgaaaaatc
     8641 ctattagtga aatgggatta cattcttata gaattaagaa aaaagaagga tatgtaggaa
     8701 tgttattagg aatatttaat tgtacagtaa tctttattat aacaataaca attggtggtg
     8761 gtcatgttac ttcatgggta ccaaaaattt cacctttaac agtttcttgg atacttgtct
     8821 ttattttagt tgcttttgca gaagaaattt taaatcgtgg tttttttatg gcagttttac
     8881 gaagatgtaa aaatatttat tttataatga ttgttccatc tgttattttt gggttgattc
     8941 atatatggaa tcctgatgtt acttttttat ctgtaattaa tattataatt attgggattc
     9001 ttttttcata tatgtttata aaatcgtcga atatatggat gtgtataggt tatcatttta
     9061 catggaatgt atttcagggg attatatatg gtatgcctgt aagtggactt caggttccag
     9121 ggattataac tacacaaatc actcatggta atctattaaa tggaggaatg tttggtattg
     9181 aaggtggaat tttgactact tttgttaatc tacttagctt tatatttgta tggtattact
     9241 atagagattc taagtatgat tttgttagta ataagtaagt gtgaataatg ttaagaacta
     9301 attaattata ataaatttta ataatatatt ctaggcttaa agtattctgt aggattgttg
     9361 gaaatttaca aaaatataaa aataacttgg acattctatt tactccaagt tatttttata
     9421 ttcttttgta aatagaaatg gaacataaaa aattaaaaca accatagata ctattagtat
     9481 atcatggtaa gtgatagttg attctttaga atttgtgata agaaaagcac tattatttaa
     9541 aacatgaatt atagcacaaa accagatatt cttagttttt atataaagat atcctaaaaa
     9601 tattgaaatt ccaactatat gtattgaacg taatattatt cctaatattg gcgcttcagg
     9661 tgtatataat gtaaatatta gtggtaagtg ccacaagctc caacacatac ctaatattat
     9721 aactcctaac tttttaccaa atttatcaaa aaaaatattc tgtaaaaaac ctctccaggc
     9781 atattcttca ctaaaaaaaa tataaatagt agttaatgat gttattggag taaatataaa
     9841 ttttatgata ccactataat taggttgagc cccttgtagt attagtataa atgatgttgg
     9901 aacaattaca gaaatcataa agtaaataat taataaagaa cctagtttaa tattttttac
     9961 aggatataaa ttttt
//
LOCUS       DAELJO010000149         9961 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELJO010000149 DAELJO010000000
VERSION     DAELJO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.149"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            23..1090
                     /locus_tag="KPB88_002899"
     CDS             23..1090
                     /locus_tag="KPB88_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG0055162.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPMKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1260..3161
                     /gene="cooS"
                     /locus_tag="KPB88_002900"
     CDS             1260..3161
                     /gene="cooS"
                     /locus_tag="KPB88_002900"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0055163.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3154..3627
                     /locus_tag="KPB88_002901"
     CDS             3154..3627
                     /locus_tag="KPB88_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0055164.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3602..4828
                     /locus_tag="KPB88_002902"
     CDS             3602..4828
                     /locus_tag="KPB88_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0055165.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4967..6154)
                     /locus_tag="KPB88_002903"
     CDS             complement(4967..6154)
                     /locus_tag="KPB88_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0055166.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6427..7251
                     /locus_tag="KPB88_002904"
     CDS             6427..7251
                     /locus_tag="KPB88_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0055167.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVYKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7380..8645
                     /gene="gluD"
                     /locus_tag="KPB88_002905"
     CDS             7380..8645
                     /gene="gluD"
                     /locus_tag="KPB88_002905"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG0055168.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8858..9448
                     /locus_tag="KPB88_002906"
     CDS             8858..9448
                     /locus_tag="KPB88_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0055169.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYVLTIVNSMKRSTNFSNKFVAGLNNQSDIRKRVMNMFSEKHKNGI
                     LIALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9778..>9961
                     /locus_tag="KPB88_002907"
     CDS             9778..>9961
                     /locus_tag="KPB88_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0055170.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTI"
BASE COUNT         3883 a         1076 c         1789 g         3213 t
ORIGIN      
        1 attaatacaa aggagattta ctatgaaaaa taaattagtt tacatatgta ttatgagtct
       61 attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga
      121 ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga gaacctatga aaaaagatat
      181 aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat catattatgg
      241 cagttttgac catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt
      301 aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac
      361 agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat
      421 tttaaaatct ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt
      481 tgaagaagga atgaaagata cacagtttat attggacgag aataaaataa attattttgg
      541 attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa
      601 tggattatca actgaatata atgaaaaaaa tttaaaacaa atggaagatg atattaagtc
      661 cttaaaaaag aaatcggatg ttgttgtagt gtactttcat tgggggatag aattagatta
      721 ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt
      781 agttatgggg tctcacccgc atgtgattca aggaatagaa aagtataaaa ataagtatat
      841 agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag
      901 ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc
      961 aaatataata ccaacttcaa taacatctag taactccaga aataattatc aaccaaaagt
     1021 attggatgga agtgagaagg atagagtttt aaataaactt gaaaaaataa gcaaagattt
     1081 aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg
     1141 tttagggtat ggtaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca
     1201 attataataa agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa
     1261 tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag
     1321 cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt
     1381 ttggacttca gggggtttgt tgtagactct gttcaaatgg accatgtaga gtaactccta
     1441 aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa
     1501 gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt
     1561 taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag
     1621 cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag gttgcagaca
     1681 aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag
     1741 cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt
     1801 cagaagtttt tgatgcaata gtaaaatcat caacaaattt aaatagtgac ccagtagaca
     1861 tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta
     1921 atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta
     1981 atgtaataga cccagactac ataaatataa tgataacagg ccatcaacat tcaacatttg
     2041 caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa tctgttggtg
     2101 caaaaggctt taagctagta ggatgtacat gtgttggtca agatttacaa ttaagaggag
     2161 aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt
     2221 tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg
     2281 aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag
     2341 cgaatgcaga atacataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa
     2401 tagaaaaagc attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa
     2461 aagaccatgg atttgaacaa tctttaactg gtgtaagtga aaagaacctg aaagcgtttt
     2521 taggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg
     2581 cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac
     2641 ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag
     2701 gattagaaaa tgttggtctt atgtcacctg gagcagaaga attagcagga gaaaacttaa
     2761 aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta
     2821 taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac
     2881 aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag
     2941 cgtttggtct tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag
     3001 gaaaattggt gacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta
     3061 tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa
     3121 gaaaaaattt agggttaaag gatgtggaat gcaatgcata gaatttttat aaataaagat
     3181 ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat
     3241 gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta
     3301 gatagtaaac ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt
     3361 gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat
     3421 gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta
     3481 aagccagatg atagaagcaa gcagaacata cttaaatgtg atttatgtaa agatgaggag
     3541 tatcctagat gtgtagctaa ttgcccaagt ggagcaattg aattacagaa ggaggaaaat
     3601 gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag
     3661 agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaacg tatattcaag
     3721 atgtatgttg catcacgtta tatctgaaca cagaacttta aaacaaataa attttgttga
     3781 tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat
     3841 agacataaat aaaaaggtag ttcagacaga agacattact gtaaattatg ataaactttt
     3901 aatagctaca ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaattt
     3961 tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc
     4021 taaaaatgta gttataatag gtgctggttt agttggtata gatgcattag tgggattatt
     4081 taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca
     4141 attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt
     4201 ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg ttacaggtgt
     4261 tgcattttca aatggtgaag ttttagatgc agacatggta attgttgcta caggggttaa
     4321 accaaatgct gactttttag atggaacagg aatagaatat gatagaggta taataataga
     4381 tgatatgtgc caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa
     4441 tgctatttgg cctctggcag taaagcaagg aattgtagct gcctataata tggttggaaa
     4501 agataagaag atagaagatg aatttgcttt taaaaacagt atgaatttca tggatatacc
     4561 aactatttct ataggaatga atacacctgt agatgatagc tataaagtgc taactagaca
     4621 tggaattaat gattataaaa agtttgtttt taaagataat gtaatctatg gagcagttat
     4681 acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat
     4741 atatgattta gaaaatagga tatttgatat atgttatgca gactttttta atataaaaga
     4801 aaatggagaa ttttgttata gtgtatagta tatacgtgga gtaaggttta taaatacagt
     4861 aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt tagaaatagc
     4921 tctatttttt gtaattaaat ttaaattata ttcaatttgt aaaatattat tttgaacaat
     4981 tatacatata ctctctagtt agaggtaaat cattttttat acctttagtg aaaagtattt
     5041 gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg tataaatccc
     5101 acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc tcagagaaat
     5161 ttttttgcca gtatagtaag gtttttacat aatgtaatct tagactttct acgtctatag
     5221 tatgatattt gtaatcacta caaatatgta tcatttctct taggctagga ataactcctc
     5281 ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat ccacttatat
     5341 agtgaagtaa aaacaatcca ccatctttta aaacagcatt tacgttttta atgaaaagtt
     5401 catagttttc tctaccaaca tgttcaagca tcccaacgct tacaattcta tcaaacataa
     5461 ggtctgattt ttttaattct ctatagtcca taagtcttac ttcaagatag tcttgaaggt
     5521 tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagt gtaattccaa
     5581 cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa ccacaaccaa
     5641 tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg tgaactttat
     5701 tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa gaataactaa
     5761 gtgtcttatc taaccataag ctataaaaat cgttgccaat gtcatagtgg gcatgtattt
     5821 ctttcttttg atgtctcatt gaatttgatg agtgcaataa atgttttaca gctgaattat
     5881 caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat aaatctccct
     5941 caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta ctttttaata
     6001 gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag ccctctccaa
     6061 ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca aatttctcta
     6121 aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct aatgtaaatc
     6181 cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt aaaatttttt
     6241 caataaattt tttatttaaa ggaaatttat tttttataaa gaaatttcat gttgtagtat
     6301 attttttgct tataaatttt ggtactggat ataatattaa actgttaatg atataataaa
     6361 tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt tataaggaga
     6421 tatatgatgg aagtatatgc tttggttggg tcaagtggca cagggaagag ctttaaggct
     6481 ttagagttcg catatgaaaa tgatatagaa tatataattg atgatgggat tttaatatac
     6541 aaaaacaagg ttttagctgg gatatcagca aaacaagcaa atactactat agaagcagtt
     6601 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa
     6661 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
     6721 gagagattgt caattggaag ggtctataag tttataaata tacaagatat aagtactaaa
     6781 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
     6841 gcagttgaga taaaaagtat ggcaagtggt cttagtataa attctttgaa gagacttttt
     6901 agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag accaactttt
     6961 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
     7021 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
     7081 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
     7141 gacatacagc agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
     7201 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
     7261 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaatttagga
     7321 aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg ggctttcaaa
     7381 tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg
     7441 ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg agagttatag
     7501 aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa ggatttagat
     7561 cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat caaaatgttt
     7621 caagagacga agtaaaagct ttatctatat ggatgacttt caagtgttct gtaacaggta
     7681 taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta tctcaaggtg
     7741 aattagaaag attaagtaga ggatacatag atggaatata taaattaata ggtgagaaag
     7801 ttgacgttcc tgcaccagac gtaaatacta atggtcaaat aatgtcttgg atggttgatg
     7861 agtacaataa attaactgga caaagttcta taggtgttat aactggtaag ccagttgaat
     7921 ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt actgcaagag
     7981 aagctgctgc taaattagga atagatatga aaaaagctaa aatagctgtt caaggtatag
     8041 gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact gttgtagcta
     8101 tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat ggtttagatg
     8161 gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc ccaggagcta
     8221 agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata ccagctgcat
     8281 tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta gtttgtgagg
     8341 ctgctaatgg accaactact ccagaggctg atgaagtatt tgctgaaaga ggaatagttc
     8401 ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc gagtgggtac
     8461 aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta
     8521 tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt acaatgagag
     8581 aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta agaggatggt
     8641 actaagattt actcttaata aataatagga tataattatt taagatataa aggggctgta
     8701 catagtacag cccttaaatt atgtggcttg caatgaagta agctattttt gttttaactt
     8761 ataaataact tgataaatta tgtaatttag aatgtatttt ttaccaccac atgattacaa
     8821 attagaagag ttagattaga tttttagaca tgaacttatg cattttaaaa gcaaagacct
     8881 taatttaaag tatctagtat tatttttaaa aatagtatat tggtttaatc catttatata
     8941 tataatggaa aaatatatgg atttggattg tgaactttat tgtgatgaga gagttttaaa
     9001 aaattgtact gtacaacaaa agcaagaata tgttctaact attgtaaatt caatgaaaag
     9061 aagtacaaat ttttcaaata aatttgtagc tggattaaat aaccaatctg atattagaaa
     9121 aagagtaatg aacatgttca gtgaaaaaca taaaaatggt atcttaatag ctttgattat
     9181 aagcttgttt tcttctatta cattttttaa attcgattca actagtatta ttgaagagaa
     9241 tgatttctta gagaggtcaa gatatacgtt tgaaggaagg caagaagaca tgtttgtaac
     9301 aatagatttt atatatgcag atgcacctat aaaacttaga aaagagcatg aagaaaactg
     9361 tagagtcaat ggaatagtac caaaagattc agattcaata gaaattattc ctagatttta
     9421 taaagattgt atggatttaa aaaaataagt tagacttgat attgatgttg atattatgaa
     9481 ttttttatat ttaagcaatt gaaaatttta ttatagattc aattttatat gtgtttaagt
     9541 atgaaattta tagatagttg aaataattaa atatgattga tgcataggaa tcattactaa
     9601 gctataatta attatagggg agtttaaata aattatgagt tacacaatta taaaatgata
     9661 aaaatagtta tctgttacaa aaaatgtatc tttttaatta ataaagataa tatgaaaact
     9721 taaaaggcaa ataaacttaa acataaaata ctcttaaata agataggaga aaaatgtatg
     9781 tctaacttta ttctaaatgg gatttttaga acagttatag taagtagtct aagcattctt
     9841 atgatattga ttttcaagaa aaatgtattt aaaagattta gcaaaaaatt taattattat
     9901 atatggatga tagttgttat taaattattt ttaccattta catattatac ttttacaata
     9961 a
//
LOCUS       DAELJO010000150         9920 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELJO010000150 DAELJO010000000
VERSION     DAELJO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.150"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..302)
                     /locus_tag="KPB88_002908"
     CDS             complement(<1..302)
                     /locus_tag="KPB88_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0055171.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFE"
     gene            complement(525..1469)
                     /locus_tag="KPB88_002909"
     CDS             complement(525..1469)
                     /locus_tag="KPB88_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0055172.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            1855..2736
                     /locus_tag="KPB88_002910"
     CDS             1855..2736
                     /locus_tag="KPB88_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0055173.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(2797..4005)
                     /locus_tag="KPB88_002911"
     CDS             complement(2797..4005)
                     /locus_tag="KPB88_002911"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG0055174.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMDLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(4078..4330)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4482..5471)
                     /locus_tag="KPB88_002912"
     CDS             complement(4482..5471)
                     /locus_tag="KPB88_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0055175.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            5623..6063
                     /locus_tag="KPB88_002913"
     CDS             5623..6063
                     /locus_tag="KPB88_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055176.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            6390..6617
                     /locus_tag="KPB88_002914"
     CDS             6390..6617
                     /locus_tag="KPB88_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0055177.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            6643..8763
                     /gene="feoB"
                     /locus_tag="KPB88_002915"
     CDS             6643..8763
                     /gene="feoB"
                     /locus_tag="KPB88_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0055178.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(8896..9714)
                     /locus_tag="KPB88_002916"
     CDS             complement(8896..9714)
                     /locus_tag="KPB88_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0055179.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNKINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
BASE COUNT         3470 a         1504 c         1245 g         3701 t
ORIGIN      
        1 tcttcaaaag ctattctctt atacgcttca gctacttctg gataaccttc tctatcagct
       61 tgtctactca ttgctaaata cattcctact tctgtacact caccagtaaa gttagctctt
      121 aatccttcga ttatttctgc atctacacct tgagctactc ctattctatg ttcatcagcc
      181 caaaccattt cgcctttcat ttcttcaaat ttatcagctc caactttaca tactggacat
      241 tgtgctggtg cagcatctcc ttcatgtata tatccacata ctgtacaaac aaattttttc
      301 ataattaatt tcctcccaat tttatataat taaattttta tttttaagtt taataacttg
      361 ctacaagaat atatataccc cagactttat agcttaaaca ctaaaaataa aatttatttc
      421 ctaattaatt ttatcacttt gaaacaccag tatcaaccaa gttttgatgt caaatttcta
      481 cataatacat aatttatttg attaattagg aaatagttgt catattaaat actttctaat
      541 gaagactcct ctgaatcttt aagtttcact tttttgctct ttaaaaaaga taatttagtt
      601 tctgcagtta tgattgcaac aaatatcaag atactaccaa taaccaattt tgctgtaata
      661 agctccttta aaattattat agaaaatatt gtaccaaata cagcctctgt ggataatatt
      721 atagctgttc tagttccatc aactcttttt tgacatatag tctgaaataa aaatccaatt
      781 gtagtactaa atacagctag atacatagtt cccatgtaac cagataattc agcttccatc
      841 tttaattgac ctgcaaatgt ttgaactaca acagacatta aaaacgctac tgtaaattga
      901 atagctgtca atgccattgg gttgttatct tttgcaaact cacttgtaaa gaatatatga
      961 aatgcaaatc cgaatgaaca tataagtgtt agaaaatctc caaaattaat agagaaatca
     1021 gcttctaaag aaagtatccc aattcctata agagctacca aactacttat tattcctatt
     1081 ttatcaagtt ttcttctata aagtataaat cctatgaacg gaactattac aacattagct
     1141 gctgttataa acgcattttt agatggtgta gtgtacataa gtccaatagt ttgtactgtg
     1201 aatgctagaa ataaaaatat tcctaaaatc cctcctgctt ttaataattt tttacccata
     1261 ttggcacgaa tctgtttaaa aaacagtaaa ttcataatta tagctgccaa gaaaaatctt
     1321 aaagttatga tttgtagagg tgtaagccca ccgtctaatg caaattgggt tgctacaaag
     1381 ccacttcccc atataattgc tataaatatt agtcctattt ctccaaagta ttttttcaat
     1441 aaagccccct cctttttatt tttttgcaaa attataaacc ccctaaacag caagagagtt
     1501 tttctataag cattactata aattaatata gtaaattttt tcagtatttt aaatctaaat
     1561 ttacatacat tttgagctca aatttatatc cactatttaa cagtaatata ttaatcattt
     1621 tttgtaaaca cctttttaat aaatttttat attttttata tttttttacc tatactaaat
     1681 ttttttatag atatttttaa tatatatgat actttaatat tatgcaaata atgtcatata
     1741 agttacactt tagttatctt agttacaact ttgtcggttt actttttcta ctttcttcga
     1801 tataattata atatattttt gatacaattc gacaaaaggg ggaatgctgt aattatggta
     1861 aaaaaaagaa aaaagaaaac tgttacactt actatacttg ttgtaatagt tattggtatt
     1921 ttttctatgg catgggcgga catgtctaga aaaaaagaaa tgaaagaaac tcaagaaaga
     1981 cttaaagctg aaagattaga aaaagaaaga attgaaaaag aaaaaaatga acctattatt
     2041 ggtgcaaaaa aagaagctgt aaaagaatat ggatatgacg caaaaatagt ttgggacaaa
     2101 cttaacaaat acgactattc aaacaatgga gaaaagatag tctttcttac atttgatgat
     2161 ggaccatcta ctactaatac tccacaagtt ctagatatct tgaaaagaca tggtgttaga
     2221 ggaactttct ttattaaggg tgattctctt gaaagaaaag gagctaatga aattttaaaa
     2281 agaacatttg acgaaggaaa tgcaatagcc catcactctt atactcatga ctataaaaaa
     2341 ctatatccta acagaagcct aaatctcgat acattcgtaa atgaacttaa caaaactgat
     2401 gaagctatga aaaaagtctt aggaaagaat ttttcctcaa atgtagttag atgcccaggc
     2461 gggtatatgt catggaaaaa tatggaacct ctaggaaact acttaaaaga gaaaaacatg
     2521 gcatcaattg attggaacgc tttaaatgct gatgcagaag gtaaaaagaa aaatgcacaa
     2581 gaattatttg aacatgcaaa gaaatcttct gaaggcaaag aaatggttgt tcttttaatg
     2641 catgatactt atggaaaaca agaaacagta aatgcattgg accaaattat aacatatttt
     2701 aaagacaatg gttatcaatt taaaatttta atataataaa aaagcaagtt tattgaatgt
     2761 acattttata ctcaatagac ttgcttttac tttactctat tttactagct cgatttttac
     2821 aaattttttc ttccctattt gaacaactaa ttcactctct tttacaatag tgcttaaatc
     2881 ctctactttt tcaccattta cctttactcc accttgagaa gctaatcttc taacttcact
     2941 cttactctta acaatctcat tagaaactag tacttcgatt aagtcaaatc catcttcctt
     3001 aacttgaatt gtttgtatat cttcaggtat ttgacctttt tggaatacag atttaaatct
     3061 ttcttcagct tctttagcag attcttctcc atggtataat gttactattt ctcttgctaa
     3121 gtccatcttt atatctcttg gatttactga accctctttc aactgagatt ctattttatt
     3181 aacttcatca ggatgaacat ctgtcactaa attgtagtat ttaattataa gttcatcagg
     3241 aatttccata gatttttggt acattattcc cgcttcttca tctattccaa tatagttacc
     3301 taaactctta ctcatttttt ctttaccatc taatccttct attaaaggca tcattattac
     3361 tatttgagac tccataccaa attctctttg taatgatctt cccattagta aattgaatct
     3421 ttggtcagtt ccaccaagtt ctatatcagc ttctaatgct atagagtcat atgcttgcat
     3481 taatgggtag aagaactcat gtactgatat tggcatttgt ccttcatatc tctttttaaa
     3541 atcttctctt tctaacattc tcgcaacagt aatagttgct gctaatttta ttacatcttc
     3601 aaaatttaac ttagctaacc actcgctatt aaatcttaca atagtttttt ctttatctaa
     3661 aactttgaaa atttgttctt cataagtttt tgcatttgca agaacttgct ctgttgttaa
     3721 agcttttcta gctttagatt ttccagttgg gtctccaatt tttcctgtaa aatctccaat
     3781 tattattact atttgatgac ctaaatcttg taattgtttc atctttctaa gaactactgt
     3841 atgccctaaa tgtatatctg gagcacttgg gtctagaccc aattttacta tcataggctt
     3901 tccttctttt tctgatttaa taagcttttc ccttaactct ttctcatcta ttaaatcatc
     3961 tacacctttc ataattattc gcatttgctc gtctatcgat ttcatttttc attcctccta
     4021 aaatatttat ataatctata atttttattt ttataataaa aaaagctttc atcctcttaa
     4081 aaggacgaaa gcattgctta cgtgttacca ccttagttca tcaatatctc acgatattaa
     4141 tctcttagag tacaattaca tatactcgtg tactataacg tgtactaatc cgatatctct
     4201 taatggttgt tatctttcag agatagtgct ctgagatgta ttcaaactaa atcaatttat
     4261 tccatttcac caaactggaa ctctctgtta aataaaatat agccctactt cttctcttca
     4321 tagcatttat atattcagat tatttcatca attacaagaa atttttctta acttactcat
     4381 aatatattat tatttttata ttttgtcaac cttttttatt attatataca tgttttttta
     4441 cattatacta cttttttata ttttttacca atacccaaaa tttatttttc aatcaaaaga
     4501 tttatttgtt tattgactat attactctga ctcaatctta tatttatttt cttatttaaa
     4561 aatgcgttta taattattaa accaataact gttacatcaa atataaaaaa cagcaataaa
     4621 attattatca aaaataaaat tctagcatat aataattttc taaacttagc tattataaag
     4681 ttttgtagtg acatacatga gtctctaatg ataaaataga cacttatata ggttattgat
     4741 ataaagattg tttttaaata aacagaatca actttaacat tcaacagttt tagaacttca
     4801 aaaatggcta gataagaaac acatatgtaa aaagcttttt ttgagcaaca taaaaattgg
     4861 tttaaatttc taaagttctt aaatattaaa aattttttta aagaattaga atctaaaata
     4921 tgcaatttat tccctaaaat taaacttaat aaatatatgc aaaacaccat accaaaagga
     4981 aacaatgcta tcattaaata ataaataaaa tttgtatacc cattaaaata aatccatttg
     5041 ctatatcctt gaaattcttt cataaaacta taacctatta atgttgaagc atatatatca
     5101 atgaaaacca ttacaataac cccaaatact gaaccatata ctaaatcatc cataaccatt
     5161 gtaggcttat ttattagata gccacacatt ataccaagta atacactctg acttgcccaa
     5221 atagcagttc ctagatttcc aagtaccaga gaaattatta aaagcgaagt aattccagat
     5281 aaaatggtat actttaaatc acatttcaag cagattatac aaaaaaatac tggcactata
     5341 aaatctaagt aaatagcata tccaaatcca gtactcacag ctataattgt tactactatg
     5401 aataaagatg acaataaagc tgcttcagtt aattttttag tagtattcaa aacactgcct
     5461 ccataaatca taaattttct aactaaagtt ttgtttttaa tatagtatat cactttttaa
     5521 aaatattttt aataaatata aattttaata aaggtattta tgttaaaaat ggtataatgt
     5581 atcttaatat tcagtttttt aagatgctag gaggtaatta ctatgtttaa aattaaacac
     5641 tttaatgatt tgagtttaga tgaattttat gaaatagcaa aatcaagata tgaggtattt
     5701 gcttgtgaac agaagatatt ttcattaaac gattatgatg atatagataa aaactcttat
     5761 cacatatttt taaaagaaaa tggattgatt tgtgcttatg ctagaataat tcctaaagaa
     5821 tacagctctt ataatgatgt ttccattggt agagttttag ttctttctag tcatagaaga
     5881 aaaggattag caaaacaaat gatggattgt gctatagatt ttataaaagt taacttacac
     5941 gaaaataata taaccttatc agcacaaact tatataaaga acctttactt atcttgtggt
     6001 tttaaagaaa tctcagaagt atacgacgaa gcaggtattg aacatattaa aatgagatta
     6061 tagtgttgac aagctaatat tgttaatata taatttaagt ataaattaaa tattgaattg
     6121 atatttaggt atttgaaaat accttacata gagtcagaag gcttttgctt tctgactttt
     6181 ttattatcaa aattcaataa ttatatggat tcaaagtgtt aatttttatt ctatattcta
     6241 ctttccccaa aagtaagaat tatacccaac tttgtatgat aggcatcttg cctatctttt
     6301 ttttgtaaaa gtaacaaggt aataatataa tgcatagttt aaaataagaa gattagccca
     6361 ggctaaattt caagcatgga ggtgctatta tgaaaaacct taacgatatt caagtgaaaa
     6421 aaactgtcaa agttgaagat atattatcta gtggtaattt aagagaaaga atgttagcgc
     6481 ttggtcttac tagaggagct gttatagatg tagttagaaa aggtccaaaa aataatttaa
     6541 ctgtctataa aatcagaggt agcaagatag ctctacgaca agaagaaagt agcttaatat
     6601 tagtttctga catttaaaaa tttctagcag gaggatatta atatggggtt gacacataac
     6661 tcaactaaaa tgagttcttt aaaagacatg tttgatatag acaataagga agaccagttt
     6721 gtaattgcat tggctggcaa tccaaataca ggtaaaagta ctgtatttaa tcatcttaca
     6781 gggcttagac agcatactgg aaactggcca ggaaaaactg tagctacagc tcgtggtaat
     6841 tttaaatata aaaataccga atatgcatta atagatttac caggaacata ttctttattt
     6901 gccttatcac aagaagaaat tgtagctcgt gattttatat gttttggtaa tccagatgca
     6961 gtaatagtag tatgtgatgc tacatgtctt gaaagaaatt taaatttagt atttcaagtg
     7021 atggaactta cagataaagt tattttgtgt attaatttaa tagatgaagc tagaaaaaaa
     7081 ggcattacta ttgataaaaa acttttagag gattctcttg gtattccagt tattttaact
     7141 gctgcaagaa atggctctgg tatggatgaa ctattggata ctttaaatga tgtatctttt
     7201 gacaaatata aattaaataa taaacctgtt agatacaatg aaaatataga gaatgtggta
     7261 aaatcaattc aaccagaatt ggataatatt ataccaggta taaattctag atggttggga
     7321 cttagactta ttgatggcga tgaaagtata tttgaatcta tgagtaatta tatagacaaa
     7381 gattctatag atgctattaa tgaagtaaag aaaaagattc cagacaatat aaataaacag
     7441 aaaataagag atgaatttac taaaatcaac tatgattatg ctaaaaaact gagtgatgag
     7501 tgttgttcta atgttgctaa aaaatcaact gatagagaag aaaaagtaga taaaatatta
     7561 acatctaaaa tatttgggct tccaataatg ctcttattat taggtactat attgtggatt
     7621 actatagaag gagctaacta tccatccact ttattatcta atttattact cggatttgag
     7681 ccaagtattt cgggtatatt aaatagtatt aattgtccat catggttaaa tgatatgttg
     7741 gttttaggat tatacagaac attagcatgg gtaatctctg taatgctacc tcctatggca
     7801 atattttttc cattatttac cctacttgag gattttggat atttgccaag agttgcattt
     7861 aacttagacc acctatttaa aaaagcttgt gcacatggaa aacagtgttt gactatgtgt
     7921 atgggatttg gatgtaatgc agctggagtt attggttgta ggataattga ctctccaagg
     7981 gaaagactta tagctatact tacaaacaac tttgttcctt gtaatggtcg ttttcctact
     8041 ttgatagcta tatctacaat attttttagc tcagttataa ccaactcttt cgtatcaagt
     8101 gtagcaactg cactttgcat aactttactg ataattctag gtgttataat aacattgttg
     8161 gtttcttaca ctctttctaa gacgctatta aaaggagttc cgtctacatt tacattagaa
     8221 cttcctcctt atagagttcc tcaaatagga agaactttat atacctctat aatagataga
     8281 acaatatttg ttttaggaag ggctgtaatg gtagccatac ctgctggtgt cataacttgg
     8341 atatttgcaa atatatatat aggagattta agtatattat cacatgttgc aaacttttta
     8401 gaccctttag ctaaattaat tggtcttgat gggtttatat tacttgcttt tattcttgga
     8461 ttcccagcaa atgagatagt tgtacctata cttttaatgg cttacctagc aactggttct
     8521 atgatagaat tagatagctt tagtgcttta ggtcaagttc ttagagaaca tggatggact
     8581 tatttgacag ctttaaatgt tatgctgttc agtttactgc attggccttg tgcaacaaca
     8641 cttttaacaa taaaaaaaga aactggtagt ttaaaatgga ctgccttagg attcttaatg
     8701 cctactatac ttgcttttgt tgtttgtttt ttaacaacta cagtttacaa tttatttata
     8761 taattagtat taactgacat tagacatata ttagagaaac tatttaaaaa tttatatact
     8821 ttattataaa gcatagagat atattcaatg ataagtaaaa ttacttaaca tttgaatata
     8881 tctctatttt aattcttatc tggatacttg gattgaaatt tcagtcatat aattatcttc
     8941 attactttca caaaaaaagt caagtatact ctcctcatac gaatttccaa taattttata
     9001 ttcattttca tatatatatt ttttcaattt ttcgtaagat ttatatgaat cctcatattt
     9061 tccaatatgt tttatgcaag catacattcc ttctggcttt acatatagct ttttatcctt
     9121 tattttttta tctatcttca aataaaaact tgatatactt aaatagtccc cattttttat
     9181 attttcatta cttactatta ctcccaactc atatccttga tacaaatttt tcgatttaca
     9241 ataattggtg aaatctatta attccatcat aaaatcacta ttactcatat tactcaaatc
     9301 gcttgataga actaaatatt cttcaaactt tttggatata taaatttctt cactattcat
     9361 tttagaaatt ccttcaactg ttatatttat tttattttgc atcttagaac ttatctttct
     9421 tagttttttt attttttctc ttatcttttt ttcttcttct ttaagcattt tcacacaaag
     9481 ctggggagtt ctattttgta tatactcttt tatttcctct aaactcattc ctatttctct
     9541 caatgattgt aatatatcaa gtgtatcata ttgataaact gagtaatatc tataattatt
     9601 tttatcttta tattcaggag aaaaaatacc tattttgtca tagaaaatta atgtctgctt
     9661 agatatacca cacaatttag caaattcacc tgttgtaaaa tactgttttt ccaagggtta
     9721 tacactcctt ttctttttgt tgtctatata gttactatat tctttattat ttactaaatc
     9781 aagtagttat tattttagaa aatcaatatg gttttgattt taaagcttaa atatcagtta
     9841 gatgtcagtt ataaatattt tacccattcc catcataaaa aagatatccc ctcttaactt
     9901 ttggatggaa tattatattt
//
LOCUS       DAELJO010000151         9878 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELJO010000151 DAELJO010000000
VERSION     DAELJO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.151"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(28..906)
                     /locus_tag="KPB88_002917"
     CDS             complement(28..906)
                     /locus_tag="KPB88_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0055180.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(923..1240)
                     /gene="trxA"
                     /locus_tag="KPB88_002918"
     CDS             complement(923..1240)
                     /gene="trxA"
                     /locus_tag="KPB88_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0055181.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1390..2622)
                     /locus_tag="KPB88_002919"
     CDS             complement(1390..2622)
                     /locus_tag="KPB88_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055182.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEISLELKEDIEDEV
                     TLLISDTGIGIKKEEISLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2612..3283)
                     /locus_tag="KPB88_002920"
     CDS             complement(2612..3283)
                     /locus_tag="KPB88_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055183.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3299..4135)
                     /locus_tag="KPB88_002921"
     CDS             complement(3299..4135)
                     /locus_tag="KPB88_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055184.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4161..4835)
                     /locus_tag="KPB88_002922"
     CDS             complement(4161..4835)
                     /locus_tag="KPB88_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0055185.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4849..5577)
                     /locus_tag="KPB88_002923"
     CDS             complement(4849..5577)
                     /locus_tag="KPB88_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0055186.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5704..6393)
                     /locus_tag="KPB88_002924"
     CDS             complement(5704..6393)
                     /locus_tag="KPB88_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0055187.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6401..6964)
                     /locus_tag="KPB88_002925"
     CDS             complement(6401..6964)
                     /locus_tag="KPB88_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0055188.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6966..7934)
                     /locus_tag="KPB88_002926"
     CDS             complement(6966..7934)
                     /locus_tag="KPB88_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG0055189.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8035..8078)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8443..9072
                     /locus_tag="KPB88_002927"
     CDS             8443..9072
                     /locus_tag="KPB88_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055190.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASGNTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9047..9451
                     /locus_tag="KPB88_002928"
     CDS             9047..9451
                     /locus_tag="KPB88_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG0055191.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDSITSATYSSNTIRKAVENALRKGE"
     gene            9454..>9878
                     /locus_tag="KPB88_002929"
     CDS             9454..>9878
                     /locus_tag="KPB88_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG0055192.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDS"
BASE COUNT         3285 a         1648 c          994 g         3951 t
ORIGIN      
        1 tctttaaaaa gttaatcaat ataaatatta agccctcttt aatttatcta aatagtaaac
       61 agcatttaaa gctgctattt gcccttgacc agctgctttt atataagaat atggttttcc
      121 aacacaatct ccagcagcaa agcatccatc tatactagtc ttcatattat tgtcaacttt
      181 aatatgaatg ccatcaatct ctattccagg aaccaatgca cttggagccg tactatcttt
      241 tataacgaat actccatcaa cttcaatgtg gttttcttta aaagatactt tgtttacaag
      301 tttatctcca tctatctgta ctggtctgtc atgtataact tcaatagaat catctaggtt
      361 atctgaactt ctcataagat tatctttttt gtacataggt ataaaataag tctttgaagt
      421 gagttcactt aaaaaatttg cttcttcttt agattcttca ttatatccta taactgctac
      481 ctttttattt ctataaagtg gtgcatcaca tgtagcacag tagcccacac ctcttcctag
      541 aaattcttct tcacccttaa ttggtctagt atactctacc ccagttgcaa ggattacagt
      601 agtagcttca tacatatcat ttccagacat tattgtaaag tattctccca tagcataaat
      661 attatttatt cttttatttt caattgaaat atccatacta tctatatgtg atttaaactt
      721 gtcttttaat tcatcaccac ttatatcata aaaacctaaa taattatcta ttgaaggagc
      781 tttaacaagc ttattactta aattatcatt accaaacatg ataattgttt tatttcttat
      841 ttttgcattt atagcagcag acaatccagc tggaccactg ccgattatag ctatatcata
      901 tctcataata ctcacccatt ttttataaat atgattttac ttgcatttca attttgttct
      961 ttggcatgaa tccaattaaa gtttctactg gttttccatc tttaaatata atcatagttg
     1021 gtactgtaga tacattgaat tgttgagcta tttctaaact ctcatctata tcaactttta
     1081 caaatctagc tttatccttc atctcttcac ctaattctgc aaatacaggt cccaacatgt
     1141 tacaaggtcc acaccaagtt gcaaagaaat caactacaac tactccctta ctaccttcaa
     1201 cactacttct aaattcagat gtatttataa cttttgccat ttttaaatcc tcctattaaa
     1261 ctttattatc cctatataat tatactatga aatttataaa tataaattta cttttataag
     1321 gtctttttat attttatact aattagtata cccattcttc atttctaaaa acaatttttt
     1381 atatttttat tattttaact tttttggtat tgacaattca aatgtagttc caatatctac
     1441 atcagaagtt acatttatat caatagataa gtttaaacta atcatgcgag ctattgaaag
     1501 tcctattcca gaaccttcta aatctttatt tcttacattt tcacttctaa aaaatctatc
     1561 aaatataaga gatatctctt ctttttttat gcctatacca gtgtcactta taagtaaagt
     1621 tacctcatcc tctatatctt cttttaattc taatgatatc tcatcaccca attttgtata
     1681 cttaaaggca ttatcaatga aaattaatat aagctgtctt aatttatttt tatctgttat
     1741 tataacttta ttcttcagtt tagaattaaa cacgaatctt ttttcctgaa actcagcaat
     1801 gtcaatatag tcttctgata tttcttccaa taacttttct aaatctattt cttctaaatt
     1861 aactttaaca atagagtcct ctttagtaag agacaataaa tcagttatca tttttttaag
     1921 tcttctagtt tccttcattg cagtagctat tgtttcaact tcatcattta ctgtactttc
     1981 tggggatttt aacatacttt ctaattttga tgaaactatt gttattggtg ttcttaattc
     2041 atgagaagca tcttgtataa acttagcttg atttttccat gctgtttcta ttggaattaa
     2101 agcttttcta gtgagatata cagccacaaa gtaagtaatt attacagaaa ttaatattcc
     2161 aattataaga acagatgtaa gctgtcttaa agaattcatc tcagaatcta tatttctaat
     2221 tatctgtatt tggtatctac ctacatctac atttaattct ctaaaagtat atccattttc
     2281 tgtgtattta aaaaacgaat tttttttatc tagtcttcta tctggaagta actcatcaaa
     2341 atattcattt tgagtatagt aacttatcct tccatcttca tatacataca ccatatcttt
     2401 aggatctttg agtcttattg ggttcaaaaa tgaagttctt ttaaattgag agactatata
     2461 ttcaagttca tcatttatct cactatcaat tccactatat gtaagtcccc taaaatatga
     2521 gtatataaat atagaaaaga ttattaaaaa actacctact acaataatat acatttttat
     2581 taaatgattt ttagtactgg taaatacatt cttatttatc atcgaatata tatcctacct
     2641 tacgtttagt tttaatatat ttatcatatc catatttact tagctttttt cttagattac
     2701 ttacatatac ttccactatt tcaatagttg catctgaatc aattccccat attctatcat
     2761 aaatctgctc ttttaataaa attgaacctt tatttaatac aaaatactca agcaagttaa
     2821 actgtttgtt ttgaagtttt atttcttctt tttcaatata cactcttttt tctagtgtat
     2881 ctaaatataa agatttaaat tctaatccgt ttctttcttt tatctttcca ttagtcctta
     2941 atatagcgta taccctagct actaactctt ccatataaaa aggttttgtc aaataatcat
     3001 tagctcctat agtaaatgct tctactttgt catctaatgt ttcttttgct gtcaatataa
     3061 gtacaggtgt atctatatta ttaattctca ttttctttag gatatcaaat ccatttatat
     3121 taggaagcat taagtctaaa atgactaaat catatatatt ttgatttatt aagtataaag
     3181 cttcttctcc atcttcacac ttttcagttt caaaatgctt gctaagttct tctactacac
     3241 tttctaaaag tatcttatta tcttcaacta ttaaaacctt cataagtatc ccctaccttt
     3301 atttgtctat cttaacttta tgagatttta agtcaatatc cacattggct ttttctttta
     3361 tatcatcata cgtatatccc actatttcaa cactttttgc ctttgttgaa cacaatatat
     3421 ctatatctgt ctcttctgaa ggctttaaaa tcttatatga tggtacacga tttactgtta
     3481 aatattctcc attttcatta acttctgctg tcttaagaat aatatttcct aaatcttttg
     3541 atgatgaatt ttttactttt acttgatacg taattccctc atttgattca tttacttttt
     3601 taagttctga tgtagatagc acctcatact gactactatt ttctagctta atattattat
     3661 ttcttatatt aatagtattt tcttttaaat taacatacac aatcttaccc tctgcctcat
     3721 attcatatgc ataaacttct acacccttta caaaagtttt atgcccacat tctatgtaag
     3781 caactttccc aggcattaaa gtcatgtcca acaaagcctt actatccgaa gatataattt
     3841 ttttattctc atcatattca ttatatatca attcaacatt ggatatatta aatgttgaat
     3901 tattttctac ctttgttgat gtatctactt tatcaccttc atctatattt ataatttctg
     3961 attttcctat attcaatact tttgcatttc cctcatctat ttttatctca tttttatctt
     4021 ctgtttgaat atcaactgaa tcaggctgtt catctttttc acatgctgtc aacataaaaa
     4081 taaatattgt taatataatc attagagaaa ttgatttttt tactatttta ttcatattaa
     4141 gcacctcata tagtagtatt ttatagtaga tttttatact ttttcataaa ttctttcaat
     4201 gcctgtgtaa gtatttgatg tttttcaaaa ttcgaattac tacataatct ttcaaaatca
     4261 ttccatattt ctttatctat acgtattctt gttgttttta attcatctat gtgttcatca
     4321 tctatatgta catctttttt attagatact tttttaggct ttatattgta atcttttact
     4381 tctaaatacc aatcatatac ttcacaaagc ttatctaaat cttcttgagt tatatttatt
     4441 tttctatctt tcttaggctt aatattttgt tttggctttg cattttttaa acctgtatca
     4501 ttaaactcaa tttgttcaac acttttactt aaagcctttt cattagaaat cttttttttg
     4561 gtgcttactt tttttctatt acttatagta ttctttttac ttttttcata ctccttagct
     4621 ttctcttctt ccaagtttct attatccaaa cttttattat aatttaattc gtcttttaat
     4681 atgtatttac ctttttctga cttatatcct tttttattta aaaaagttct taaagtactt
     4741 tgactaatct ttagattttg agctatctct gcaaatttaa atcctttgct ttgaagttct
     4801 aaaaatttgt caattctgtc ttcattgtta ttcattaata ttcctccgtc aacatcttct
     4861 tgtctttctt ttcatacttc ttattatatt cttcttttct aaatctactc tatttgattc
     4921 aattattttt tgtagcattt tattataagt gaaatcatct aaattattat tttttatata
     4981 gagaaaagtt tcctcttcac atttaataaa acaaattgat agcaaccatg ccatacccat
     5041 tttaacataa tattcttcac tttgtatatt attacagtat tcaaatattt ctttaatatg
     5101 tttatcatca acataatata ttaaaaacat aactaatgca aatctaattt cccatggatt
     5161 ctttgaataa acatattttt taagtatatc atacatattt tctttatatt ttttcataga
     5221 ctttaaatca gaacaaaatc catcgcaaac tgaccaatta tcgattttag gtataaatct
     5281 ttttacatag tataaagttt cttcaaaatt actatttata ttgccaatta ccataccttg
     5341 tagcctaact tcttcatagt atgtatcctc tgctacttct aaaaattcct tccaatttcc
     5401 ttggcttatt tcttttgata actttcttaa attaggaact ctaataccta ttatattttc
     5461 tgttccaggt ataagctttt tattaaaatc tctatatttt atatcttgta tagacatcat
     5521 atattctaaa agttcattat aatccaattt attccatttt cccctatcaa acatcattta
     5581 attgctcctt aattcctttt ttcttattgt aacataaaac tctacaaaaa aagcttctaa
     5641 ctattagaag ctttttcaaa aacataagaa acttttcaat tttaaattta aactaaatta
     5701 atgctaatct cctacaccaa agtctgtatt attgtataaa acttctatat ttggattttc
     5761 atccaagaat ttatacttac taacaaacca tttcactaac tcttcatctc tttttatact
     5821 agaagaatta tttataaaaa cattcactgt cgctttttca aaagtattta ggataatctc
     5881 tatatcttta tctatcatct cttttgtttg tccttttatt ccaaccataa tacaaggtga
     5941 atcaaaatat tcttttacat cttgtggagt cttaaaattt gcatttttat ttaaaatatt
     6001 atttctaaaa tcattgtcaa aggtttcaac tccaatttta aataccacag gaatttcaaa
     6061 atactctctc atttccttaa gcctattttt gtaagaccaa tgactttcta aaaataattt
     6121 ttttatattt ttctctttta taatacactt tattttttct aaagtatcct taggtaattc
     6181 aaaacagctt ccagaattta tgacttcaag aactccatat ttcccagtta cgttttttaa
     6241 aacctttaga tttaatttat tcatctcttc ttctaaatta gaattatcat ctatataatc
     6301 acaaaaagaa cactttcccc atatacaagg aaatccttta agcagaacaa tttctctttg
     6361 atttttattt gttatctcac tatatctatc cattttctat ctattttcct ccagtcaatg
     6421 atacctttaa agtttttgga actgcattta atcctaaatt cactaaaaca actgctaaca
     6481 gcttatctcc ataatcagta aatacttggg ttacaaacac actaaaaaca tctcccagtc
     6541 ctaacacttt caagatttgc actatatatg atgaacctga agaggtcata cctccaaata
     6601 aaaatgctga aattattgaa cttattatag atgttggaat ggcaaataaa agtactccta
     6661 gtggagtttt taaacccctt aaaaaattct ttttaaatgc tattccagca agtaatccgg
     6721 ttgatatttg aactggtgca aaatagagag aatacacatc aaacgtaatt ccacttatta
     6781 aacttccaaa cacacctgta acaacagcgt atttaggtcc taaaagtgaa gctatcatta
     6841 tagttcctat tgaatctaag tatacaggta atcttaatcc catagctata aaagcgccta
     6901 ttatatttat agaaatacca aaagctataa gtgtaatagt ttttacacat atttttcttt
     6961 tcacactata tcactccttc cacagagtct ataatgtctt cataaccttt aaaaagcctc
     7021 tttaaaaaca tgtacataaa tttcttctca tcaacttcat ttagaacaat tgcattagga
     7081 ttttttttat agaaattgaa agaatccact atagactgac ccatagctat tccatcttct
     7141 acaacttcta catatgattc aaatccttta catatacttc tatctataaa gtatgctact
     7201 gctagagggt catttatcac acagccaatt attccctcct gttcccaatg gaaatcaata
     7261 taaaatctag ttatttcagt tatatacttt gccatcttct tatcaagtct atttataaac
     7321 tcaataatat taggagtaag tacaattttt ctagttacat ctaaacctac catgtggatt
     7381 tttttagata aattcttgta aacataatct gctccatgtg ggtctaccca ataattaaac
     7441 tctgctactg gagagcaatt tccatgaatc ctaaatgctc ctcccataga tacaaactca
     7501 tcgagatttt caaatgcttt cttatcttta ataagtgctt tagctatgtt tgtaagtggt
     7561 gcaagtgcta ttattgatac tttttcatga ttatataaag tatttattat aaaatccact
     7621 gctccatttt ttgctttagc tcctacaacc ttttgataaa agttttctcc aataccatct
     7681 tctccatgtg tatcttgagc tgttacaagt tttcttttta gtggtgcttc ttctcctata
     7741 tatactggaa tatttagtga agaacacatt tgaagtgttt ttagtgcatt ttctgcacct
     7801 atatttgctg gaacatttcc acaacatgtg gtaattccaa ttacttctag ctctggtgag
     7861 tttaaagcca gaagaattgc caaagaatca tcaattcctg ggtcacaatc aattattact
     7921 tttctctttt ccatttttta tcctccttga tttgtggtcg aggagaacta cacatctttt
     7981 ttccactttc aaaaaggtgt gtctaagggt attaattata tactgccact atataattcc
     8041 ttagtttttt atacttggag ttctcgaaca agtacagatg taaaatcata tttttaggct
     8101 acttactata gactcttaaa aactatagac tgttaaaaaa tttttaaaca tctgatttaa
     8161 tagctataaa ataaatttta tagcataaaa gaatttatta tattttaatg tatcttatta
     8221 aagtttattt agaattatga accaacttta ataagatgtg gcgcatttaa accgattatt
     8281 attatacaat aatatcttaa atattacaat taataacatt aatttattat ccataaaaat
     8341 atattaactt atattattta ataatttatt gacagtatta aaattaagtt atacaataac
     8401 agtgtaaatt aacttaacac cgttaagcaa agaggtgatt atatgagtat caaagaaaga
     8461 cgtaaaaatg aaaaagaaga aatgaagaaa aagattatgg atgctagcat cgaaattata
     8521 aaccaacatg gatatgaaaa tttatcaata agaaaaattg ctacaaaaat agaatattct
     8581 ccaactacaa tttatcttta ctacaaagac aaagcagaga ttatcagcga tatgaccaat
     8641 aaactttata acacagtaga aagtaatgct attgacatta tgaataattg ctcatcacta
     8701 ccaatagata aacaaatcat agaaattatg actagcttta taaaaactct ttctagtgaa
     8761 ccagagatgg caaaagctat catgcatagt agaatgaata ttatatttgc atctggaaat
     8821 acaaacaata cgcctagcaa taatggtata aaaatgcttg ataaattcct ttccattgga
     8881 attgaacaag gaatattcaa aaaaaatata gataaaagct catggatgct tataagtgca
     8941 ttgttaggat tcgttttatg tgttgttgaa aataaactat attctttaca taatttttct
     9001 caacttatag aaaatttctt agatatttta gtgggaggac tttacaatga aaattcgcaa
     9061 caaggctact aataaaaaga aaaaatatgt aaaaatatct attgctctat tctttgtatt
     9121 tatatgcatt attattgtaa ttgtaagcaa cttaaagcca gaaaacttgg tagtaaaaga
     9181 tatagatatt aataatgtta aaaatggcat ttatactgga agtgctgata ataacttggt
     9241 aaaagctact gtgtctgttg aagttaataa tggtaaaatc caaaatatta atatcttaaa
     9301 acacgaccat cttttaggta aacctgctga aaaaattaca acaagtatta ttaaacaaca
     9361 gtctttagat gtggattcta taacttctgc aacttatagc agtaatacta tacgaaaagc
     9421 tgtagaaaat gctttaagaa agggagaata attatgaata ctattattat atattcaagt
     9481 aaatatggtt gtacaaaaga ctgtgcaaat atactaaaaa ataaactatc agataatgtc
     9541 actattgttg atattaataa taataataat aaaattgagt tatcaaaatt tgataaaatt
     9601 attattggaa gttctatata tgttggttca gtttcaaaaa aaatacaagt gctatgtaat
     9661 gataatgtag agttattaaa caaaaaacaa gttggcattt tcctttgctg tggcttttct
     9721 gaacaagcag ataaatattt aaaatcaaat tttccatcat ccttattaga aagtgcaaat
     9781 gctattggaa tttttggtag tgaagctcga ttagaaaaaa tgaaattttt agataaactt
     9841 attatgaagg ctgtttcaaa aggtaattat gacagttt
//
LOCUS       DAELJO010000152         9304 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELJO010000152 DAELJO010000000
VERSION     DAELJO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.152"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            10..657
                     /locus_tag="KPB88_002930"
     CDS             10..657
                     /locus_tag="KPB88_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0055193.1"
                     /translation="MEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLINEIGEIIGED
                     MPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRKGVKITATAI
                     FTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDTKVLAASFKN
                     AQQVIELMEQGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKGSKTNNI"
     gene            complement(779..2173)
                     /locus_tag="KPB88_002931"
     CDS             complement(779..2173)
                     /locus_tag="KPB88_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG0055194.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYYNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2251..3597)
                     /locus_tag="KPB88_002932"
     CDS             complement(2251..3597)
                     /locus_tag="KPB88_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0055195.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      3847..3949
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4043..5419
                     /locus_tag="KPB88_002933"
     CDS             4043..5419
                     /locus_tag="KPB88_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0055196.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(5516..6661)
                     /locus_tag="KPB88_002934"
     CDS             complement(5516..6661)
                     /locus_tag="KPB88_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0055197.1"
                     /translation="MNTFEQLKISSTIIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            6914..7621
                     /locus_tag="KPB88_002935"
     CDS             6914..7621
                     /locus_tag="KPB88_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055198.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            7668..9263
                     /locus_tag="KPB88_002936"
     CDS             7668..9263
                     /locus_tag="KPB88_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055199.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNVYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
BASE COUNT         3353 a         1179 c         1384 g         3388 t
ORIGIN      
        1 acaggtaatg tggaggagat taaggagtta tgtacttgtt taccaataga tggtgtaact
       61 acaaatccaa gtattgtttc aaaagaaaaa aagaatttta agcaacttat aaatgaaatt
      121 ggtgagatta taggtgagga tatgcctata catgctcaag tgttaagtac aaagtatgaa
      181 gaaatattag aagaagcatt atatataagt agtttgagaa aaaatatata tgtaaaaata
      241 cctgtaacac aagatggttt aagagctata aaagaccttc acagaaaagg tgtaaaaata
      301 actgctactg caatattcac tgctcatcaa ggttttttag ctgcaaaggc tggagcaaat
      361 tatattgcac cttatgtaaa taggcttgat aatatatctg gtgatggagt agctatggtt
      421 tcagaactta taaaaataat agatacttat aaaatggata caaaagtact tgcagccagt
      481 tttaaaaatg cacaacaagt tatagaatta atggaacagg gggtgcatag tgttacagta
      541 ccatatgata tttgtaaatc tatgatgaat catccattaa ctgattggag tgtagataag
      601 tttatagagg attgggagaa tacttttgga aaaggaagta aaacaaataa tatttagtat
      661 tatggtaaaa gagtagtttt gtccaatttc acaagaactt atataaaaag atgaaaatat
      721 gaattttctt tttaataatt tcgttattat cttaagttaa ttcatatttt catctttttt
      781 agatttctag tcctataaat ttccaccaag ggaatgttat acatattatt ataaagtagg
      841 ttaaaggaat caaagttaaa ccgtatttga aaataacctt gctattatac agattttcac
      901 catgaccaac cattattgta gcatgatgaa atggtaatat gtactgtatg cttactataa
      961 tgtagacaaa tagacataaa gtaacactat tttcctttag tatatgtaaa cttccaagta
     1021 atggtattac tacagatgat gtagtgacag cacttccaag acatatattc aagattattg
     1081 tgagagttat caaaaacaat attgaaataa tttctgaaga gtttggtata aactttatca
     1141 aataatttcc cattatatct acaactccag agtactttaa cactcctcca attgaaaatg
     1201 ctgctataaa aaaaataagg accttccaat ttatttcttt taaagtacta aatccaacta
     1261 tttttcttag taataaaaga agtacacaaa ttgacattat aattaatgtg tttatgttat
     1321 gtatgaactg agttaaaaac ataaaaaaca ttattcctag gcataaaaat acgtgttttt
     1381 gctctttgtt tagcaaatta aattcttttt ttaaattata atatgaaaaa tgaagtttat
     1441 taccctcaag attttcatta gaaataaatt ttattgaatg caattctttt ttaaagacaa
     1501 agataaataa aaaataagtt ataaaacaag taattatagt tggaacagac atataaaaag
     1561 cccattgacc ccaatttata ttacaatttc caagttctag tacaacataa tttagaaggg
     1621 tgtctccatt tacaaaaaac attgatgtaa aggtagatgc tgtaaatata ctaaataaaa
     1681 gtatagattt tgaatcttca gttatctgtt gttcttttaa aaactccttg taaaaagcag
     1741 atacaagaat aacccttgga aatggttgtg ggataaaaaa tattaaaagt atacctaaaa
     1801 tatacgataa aagaatcatt tttttagggg tatttacagt atttaaaatt aacttctcag
     1861 aaataatttt tgcgacacct gtttttgtaa ctgcattagt aattatgtat gcaagaatta
     1921 ttatgtaaaa attttctgta aataaaaact gtaaaataac ctttattggt gcaatgttaa
     1981 atataaacgc tagaattaca aaaaacaatg caacaaaagt tttttcaact gagtctacag
     2041 cccatatcaa gatagttaaa aaagataagc atatagaaaa ttttatgttg aaactgtaat
     2101 taagagagaa gtagagaatt atggtagcta taacagtcat ataaaattgt tttggcttaa
     2161 gaataatacc catttttata ttcacctcca taatttatta tatcataatg gaggtgaata
     2221 caatgaatgt gaatacattt tataaaaaga ttattcattg attctatgat ttacaatagc
     2281 tttagttttc caatgtcctt tcttatagta gataactgta acaataagac ctaaagtcca
     2341 accaaaagga atacccatcc aaattccgtc tgttccaaaa aatttagaaa gcatataaga
     2401 agctggaagt cttccaagcc ataaagatac tattgagctt ataagtgcaa acatggaatc
     2461 accagcacct ttcattgcac ttgaaagtac aaaagaagtt ccaataaata aaaagaatgg
     2521 tcctattata aataagtaag aagagccaat tttaatgaca tctttatcag tattgaatag
     2581 agcaattaga ttttccctaa atagcaaaat taaagcaata actgtaactg ctaaaactaa
     2641 ggtcattttt atagattctc gaatgccttt tttaactctt tcattttctc cagcacctat
     2701 gttttgagca acaaaagtag aaacagcaga accaagattc ataataggta agcttataaa
     2761 attttctatc ctaagaccag caccaaaagc tgccatagca gatgtaccat aactattaac
     2821 taaaaattga agagccatat ttccaattga aaacaacatt tgttgaactc cacttggaag
     2881 tcctaatcta agtccatctt taaatatttt gttatcatat acaattcctt tgattttaaa
     2941 tttaagcact tcatgttttt tatttaaata aattatactt attataaatg atactgcctg
     3001 agccattata gtggcaagtg caacgccctc aacacccatt cttagcacta caataaaagt
     3061 taagtctaaa actatattta gaattgtggc tattatcaga aaataaagtg gagttactga
     3121 atctccaagt cctctaagta ttgcacttac tgaattatat ccagcagaaa ataatattcc
     3181 actaaatatt atagtaagat acaattttga ttgagcaaaa acggattctg gtgtattcat
     3241 aagcttaagc atggggccac caaatactat tccaagaata cttattaata tagaaccaat
     3301 aaataaaagt atatatgtag tatcaatagt agcttttacc ttttttaaat ctcctgagcc
     3361 ataaaattgt gacactaaaa tagaaaatcc aaggctaaca cccataagcg ctgccaccaa
     3421 taaaaacatt atagggttag ctccagagac tgccgccatt gcttcttttc caataaatct
     3481 accaactatt atactgtcag ctgtattgta tagttgttga aataaactac caataagcat
     3541 aggcatggca aaaaagaata tggactttcc ctcatgacct gtagttaaat ctttcacctt
     3601 attcctccaa ataaaaaaat tattaattta ttatagtata atcttaacat aaactttata
     3661 tccaaactat aaataaattg tatcaaaata tcatatattt ttttaaaatg taaagaatat
     3721 aattataatt tgaatttaat aaatcaatat ttaatataat cgaatattaa tacaaaatca
     3781 atacaatata tacgtatata ttgacaaaat taattatata aattaaaata atattcgtaa
     3841 agaaaaattc atgttttgta ctgtataaca tataattttg acaatatggg tcataagttt
     3901 ctaccggaat accgtaaata ttctgactat gtatactatt atttagttaa ttaaatattt
     3961 aattaactga attatgaagg atacggctca gcttatcaac tactgtgtcg tttttttgta
     4021 aaatttaata ggaggtgttg ttatgcaaac aacaaacagt agtactatgc agaaactttt
     4081 tccaattttg actaataaaa atgtcgacat gaagaaggaa ataattgcag gagtaactac
     4141 ttttttaact atggcttata taatagctgt aaatccaaat attttatctg agacaggcat
     4201 gcctgcaggt gcacttgtta caggtacttg tttagcagca gcatttggat gtattttaat
     4261 gggtgtagtt gctaaccttc cttttgcttt agcatcaggt atgggtctta atgcattttt
     4321 tgcatataca gtagttttac aaatgggtgt tccttgggaa gtagctctta cagcagtatt
     4381 tgtagaaggt attattttta tagtattatc agtaagtgga gttcgtgagg ctgttgtaaa
     4441 tgcgatacca aaaaatatga aattagctgt tacaggtggt ataggaatat ttattgcact
     4501 tataggtcta gttaacagtg gaattgttat tggagaccaa gccactctta tcaaaatggg
     4561 tagatttaca ccagcagtaa taataacttg tgtaggatta ataattattg ctgtattaga
     4621 taaaaaaaga gttaaaggtt ctatactttt tggaatagta gtgagttcat tacttgcttg
     4681 ggcatttgct tttataaatc cagagtatgc acaaaaatta ggtatatatt tacctggtgg
     4741 attatttaaa ttcgaaagtt tagcaccaat agcaggtaaa atagatttag gatatgtact
     4801 acatccaaca aatataggtg gttttttagt agtagtttgt acatttttat ttgttgattt
     4861 ttttgataca gtaggtacat tagttggtgt ttgttctaaa gcaaacatgt tagatgaaaa
     4921 agggaatgtt cctaatgtag gtagagcctt acttacagat gcggttgcaa ctacagttgg
     4981 tgcaggactt ggagtatcta cagttacaac ttatgttgag agttcaacag gagttatcgc
     5041 aggtggaaga actggatgga cagcaataac agttggaatt ttattcttag ctgcaatgtt
     5101 tttctctcct gtatttatag caattccatc atgtgctaca gcaccagctt taatatatgt
     5161 tggatattta atgcttggta cagttaagga tatagagttt gataatataa ctgaaggtgt
     5221 tccagctttt gttactattg cttgtatggc attaacatat agtataggtg atggtttaac
     5281 tttaggtata ttaacttatg ttttcgtaaa tatattttat aatatatttg gagctaaaaa
     5341 ggcagaagac aaaaaacatg tttctgtagt aatgattgtt ctggcaatat tatttataat
     5401 aaaactttgg ctactataat aggtattatt taaaatttta atttaatata aaataaaggg
     5461 ctgtaattta ttatcaattg tgatgataaa ctacagctct tttgtattta ttgtattatt
     5521 taataatgtc tattagttct ccattttgta aagttttagg tgtaattact atattgaact
     5581 ctttttgcaa atcttttata atatctaatt ctttttttgt tattattgag attgtgttac
     5641 cacttcgatt tcctctagca gttcgaccac atctatgcag gtactcattt ttactttttg
     5701 gaaaatccaa gttaaaaaca tgacttacat ccacaatatc tagtccacga gcagataaat
     5761 cagttgtaat taaaatcttt gctttaccaa gtttaaattt atttattgca ttctttctat
     5821 cttctttttc catatttcca aaaattccta ttgctttgta attatgataa tttagttttg
     5881 atactaggac ttctatattt ttttcattat ttacaaatac aatagctttt tttggatttg
     5941 tagctgctaa tgcctttcta agaaaagtaa atttatccct tatctcacct aataaatatg
     6001 agtgattgat gttaggattt atttgtgatt tttcctttgt ttttattatt tcaaactcct
     6061 tcataagttt atcacaaatt tttatagtgc tatcagtaag acttgcagag catcctataa
     6121 tttgtctatc tcttaacgtt gttcttatta tgtcttctat acaagttata ttttttccat
     6181 tcaatagatt gtccacttca tctaagacaa tagtttttat attatgtgat tttagtttct
     6241 tttgttttat caagtctaaa actcttccac aagagccaat tactatgtgt ggttttactg
     6301 cttttatatt ttttatttgt ttttgtatat tgacctcccc aattaaagct aaagaagtaa
     6361 cggagagttc tgcattttta gccaataatt caacctggtt tgtaatctgc attactaatt
     6421 catgcgtagg tgcaagtata agagcttgtg tttctctttt tgatgtatca attttttcaa
     6481 atataggtaa aaggtacgct agtgtttttc ctgttccagt ctgtgaatta atcaataaat
     6541 ccttattttg aattatattc cctataacaa gtgactgaac ttctgttgga gaagttatgt
     6601 cttgtttttt gagtccatct attatagtgg aacttatttt taattgttca aatgtattca
     6661 tattttactc cttatatttt aataattata gtcaactatt ttttattata cattgtttat
     6721 tcaaatcgaa ctatatattc ttttataatt atgtgtaatt tgaaagtaaa tatcaattat
     6781 atttatacct ttatatttct gatacctttg cattttatag atttctaaaa taaacaaata
     6841 aaagttgtaa ttgtttgata tttgttatat attatatata acaaatatca aaactataat
     6901 atggagggaa attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt
     6961 agtaagtagt gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa
     7021 tggagcaggt aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg
     7081 agaggtacta ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat
     7141 ggcatatata cctgataatc cagatttata cgagtcatta actggtatac aataccttaa
     7201 ttttatagct gacatatttg aagttgccaa agataaaaga gaagagttaa taaaatatta
     7261 ttcaaataag tttgaactca ataaagcatt aggagattta atatcttcat attcacatgg
     7321 tatgaaacaa aaattagcta taatttcggc tttgatacac agcccaaaaa tcttaattct
     7381 tgatgaacct tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag
     7441 ggagttttgt gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga
     7501 aaaaatttgt gataagattg ccataataaa agatggaaat ataattgctt atggaactac
     7561 agaagaagtt aagggaagta attcacttga aagtatcttt atggaattaa tagagaaata
     7621 aaatgttttt tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa
     7681 gtatattaat aaaaaataat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg
     7741 atggtagaga aaaacaaaaa ataatgggaa tgatgctttc agttgttata atagcgggtc
     7801 tattaatttt ttatgtaacg agcttaagtc tctctttagt tgatatctta aagcaaataa
     7861 atcaactgga aatgctactg atatttggtt ttggattatc aacattaaca actattgtta
     7921 catcattata taaaacgtca tcatatctat ttcaagcaaa agatttagac cttttgacta
     7981 gtttacctat aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa
     8041 actatttatt ttcttcaata tgtatgatta tacctgcagt ggtttacttt ttaaattctg
     8101 atgtaagtat aacttatttc ccttatttga tattgctatt tttagcttta ccattattac
     8161 cgatagttgc ttcatcaata atttttctat ttatagggca gatatcatca agaataaaat
     8221 acaaaaattt agtattaatc ataggtagca ttatattgat gttgctttat accctactta
     8281 tgtcaagact taattctatg tcagttgaaa tgctaaagaa tagtgtgtct atttcagaga
     8341 cgattagaaa agtatatcct cctatatatt attttgttga tgctcttaaa acaggaagta
     8401 tagtttcatt tttagcattt atagccatct caataatacc attttttatg tttgtaactc
     8461 tgtttgcaaa aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa
     8521 attatgaatt taagggtcaa aaaacttcaa gaccttcaaa agctttatta aaaaaagaat
     8581 ttaagagata tacttcttcg tttatatact ttttaaattc atctgcaggt ttgttatttc
     8641 taatagtagg tacaatttta atattattat ttggagcaga taaagtgggt gagttactga
     8701 aattgaatat agattttaat cttataaaaa tacaattatt gggagtattc ttctttataa
     8761 tagtaatgaa ttgtacaaca tactgttcaa tttctcttga aggaaaaaac ttatggatac
     8821 tgaaatcttc tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt
     8881 tattgaatgc cccattatct attttatgtc taatattagt atctataaga ttagactttg
     8941 aaattcagtt tattattgta tcaatctgtt tgattgtaag tttttcaatg tttgtagcat
     9001 tgatgggatt attatcaaat ttattatacc caaaccttga ttggaaaaat gaggtggctg
     9061 tagtaaaaag aagtgcaagt atgatagtta caatgttagt atcattagct tatgtaatta
     9121 tacttggagg tatatatgta ttacttggaa tttcgttttt aaatgtatat attattttgg
     9181 caagtatact tactttgata ttagatttta tgatatggag aattatttct actaaaggag
     9241 taaaaatatt taaaaattta taggtgtatt tatgtcaaat aaagacttat cttttttagt
     9301 cttg
//
LOCUS       DAELJO010000153         9175 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELJO010000153 DAELJO010000000
VERSION     DAELJO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.154"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            231..1310
                     /locus_tag="KPB88_002937"
     CDS             231..1310
                     /locus_tag="KPB88_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0055200.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPB88_002938"
     CDS             1634..2002
                     /locus_tag="KPB88_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0055201.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2916
                     /locus_tag="KPB88_002939"
     CDS             2119..2916
                     /locus_tag="KPB88_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0055202.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDTPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRNMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3308..4198
                     /locus_tag="KPB88_002940"
     CDS             3308..4198
                     /locus_tag="KPB88_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0055203.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECACYGILMD
                     MKEIIKAYKRSESYTQIKFF"
     gene            4461..4904
                     /locus_tag="KPB88_002941"
     CDS             4461..4904
                     /locus_tag="KPB88_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055204.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5076..5246
                     /locus_tag="KPB88_002942"
     CDS             5076..5246
                     /locus_tag="KPB88_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055205.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5620..6741
                     /locus_tag="KPB88_002943"
     CDS             5620..6741
                     /locus_tag="KPB88_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0055206.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            6946..7434
                     /locus_tag="KPB88_002944"
     CDS             6946..7434
                     /locus_tag="KPB88_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG0055207.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7520..7927
                     /locus_tag="KPB88_002945"
     CDS             7520..7927
                     /locus_tag="KPB88_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055208.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGISLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            7928..8146
                     /locus_tag="KPB88_002946"
     CDS             7928..8146
                     /locus_tag="KPB88_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055209.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            8791..9096
                     /locus_tag="KPB88_002947"
     CDS             8791..9096
                     /locus_tag="KPB88_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055210.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT         3659 a          903 c         1577 g         3036 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atggtttata gtttaacagt aggaaatatc
      121 gtatgagtaa gactatcata ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcact agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaataccc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agagtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttagtatggg acactcctgc tcatggaaaa tcaagacctt atttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggaa tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atacccatta
     2941 ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gtataataca aagcatgata tataaatttt
     3061 taagcgaaat acatcaaaag aaggattaga attagtggag ataaaacagc tattagagat
     3121 tataaataaa tagttttaaa ggtactacga atatatagga aggtaaatca tacgttattt
     3181 aaggattata aatgagtagt ttaaaagtac tatcggatag gtctctgcat atttaaacct
     3241 tagtaaaggt agttaaagtg gtagtttaaa aactaaaaaa ttatataaag gaattaggta
     3301 taagaaaatg attgagagta taccagcaaa acaaatttta caaaaagtga aatttgacaa
     3361 tacaagatgg tttggaattg actataatat gaatctctat cgtggttgtt ctcatggatg
     3421 tatatattgt gatagccgta gcacaatata caacatagag aattttgaca aagttagata
     3481 taaagaaaat gtgatagaaa ttttaagtaa agaattgcgt tcaaaaagaa aaaaaggggt
     3541 agtaggcatt ggagctatgt ctgacacata taatcctttt gaaaagcaac tttgtattac
     3601 aaagcaggca ctagatttga tttcggaaaa tcattttgga gtgtctattg atactaaaag
     3661 tagtttggtg gttagagata taccgatttt gcagaaaatt aaaaaaaata attctgctat
     3721 tgtaaagttg actataacca ctgcaaatga tgaattatca aagaaaatag aaccttatgt
     3781 aaatccaagt tctgtacgat ttgacgcagt gaaagaactt aatgattctg gaattttttg
     3841 tggcatatta ttgactccta tgcttccatt tttgactgat aataaagatg aaatacgagc
     3901 aattgtagaa aaagcacaca aggctaatgc aaaatttatt tattgcatgt atggtgtaac
     3961 aatgcgtagt ggtcagagag agttttttta tgagcattta agagatatat caccaaaatt
     4021 ggtacttaaa tatcagaaaa catatggttt aaactatgta tgtactgtac aaaataaaga
     4081 ttgttgtgag aagttgttaa gagaagaatg tgcatgttat ggtattttga tggatatgaa
     4141 agagattata aaagcgtaca agaggtcgga gtcatatact caaataaaat ttttttaaga
     4201 taggctttgg ataagatata atagtggtga ttgatgtgtt gatattatag ttgattgtta
     4261 gagaggatat tatagatgaa tattagtatt gatattctag gtaattgcta gagctgatat
     4321 tataatttat tattagtatt aatattctag ggaattatca ctatatttta aaaagtaaaa
     4381 atatatatca aaatgaatta aataagtact gtaagaagtt tgttaaatta aggtttgaat
     4441 aaagtgaggg tggagaactt atgatagaaa taaaaactat agaaataaat aaaaaaagtc
     4501 aaattgacct tttaatgtta gccgacccat gtgaagaaat gattgataaa tatctcaata
     4561 aaggaactat gtacgcattg tatgataata aagaacttac ttgtatagca gttgtaaatg
     4621 aaatttccaa agaaatatgt gaattaaaaa acattgctac atatgagcat tttcaaaata
     4681 tgggttatgc ctcaaagatg atttatcatc ttttagatgt ttatagtaaa aaatattcat
     4741 caatggttgt tggtacatct gaaagcggag ttccttttta tgagaagttt ggatttgttt
     4801 attcacataa aattaaaaac ttttttgtag ataattatcc agaaccaatc tttgaaggag
     4861 aattacaatg tgttgacatg ttgtatctat catataactt ttagatagga taatattttt
     4921 aggtagatat aattaattaa ttcatataat tatcggaatt ataatttata aattatgtat
     4981 aataagagta taatttattt agaagctgtt atattgtatt agattcaaat aagatatata
     5041 ataacttgtt tattttgaat tttggaggat taattatgga ttcaaataat aatactataa
     5101 aatcaactgt taaaaagggt atttcttttg gttcttgttt agcaatgatt atttcttata
     5161 ctgcatggaa atctattcca tgggctattt ttcatggctt aatgagttgg atatatgtac
     5221 tttattattg ggttaagtat gcatagatta aattaataaa taagtatgta atattgtaaa
     5281 gtttaagaaa caaaatgtaa gggagtgtct cgaagtgaac tttttagttt ataaggcact
     5341 cttttttgta ttaaataaaa tatattttca ttacttaaac aatgaaaaag atgagtatct
     5401 ctattttaat acatactctt atattcattc atttatcaac aagagtttta aaaagaaagc
     5461 tatctaaaat ctattgtaat ctaagtgctt ctctttaaag tcttaacttt agtaatgata
     5521 tataagttta taaggttctt atttcaataa tgcaaatttt acttagaagt tcagactttg
     5581 gtatgaagaa atgggtaata ataaaaaaga ggtgttgata tgaaagaaaa attagaagaa
     5641 aaatctaaga ttattaaaac atcaaaagag tataaacgaa ctataattga agaaaaggac
     5701 aaagaatgga ccaaaaaatt aaatgacata cttagtagag gtgatttttc taatgaaaaa
     5761 ttagataaaa tcaaggattt aattccaaaa gaaatattga ctagtgaaaa tgttggagaa
     5821 gttgtaactg aagatgggta tgctaagcct gagtatgatg aggtatttaa atcattgttt
     5881 actttaaata atgattacga tttacttgca aattttataa atgatatcct caaagatgct
     5941 ccatatgcta ataggaatat caaacagttc actcaaataa aaagaataat taaggttgaa
     6001 accgacccaa caattaatta cattggtgag aaaagaccta gacttgatat tttggctgaa
     6061 gatgaagaac gtaaccatat aaatattgag atgcaaagag ctcttgaaga ggattatttg
     6121 gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga aggtaaagaa
     6181 tataaagaaa ttggaaaaac tgttggaata catatactaa atcatgtaaa atataatcac
     6241 atagaagatt atgtaaattg cctgagatta acaatggacg ggcatccaga tattttttct
     6301 agtaaaactg cattatattt tatagaactt ccaaaaattc gtaaatccag ttgtatagca
     6361 aacagagttc taatatgggg taaacttatt gataatcctt ctcatataga tattagaata
     6421 ctatctaaaa cggattatgt tattaaaaga gcacttgata gattaaaaga attaggtagt
     6481 aatgaggact atttattaaa tttaaagaga ggtgcgtata ttatgaataa aagtagaaat
     6541 tttaaagaag aaatttttca agaaggtatt gaaaaaggta tcgaaaaagg tatcgaaaaa
     6601 ggtattgcaa aagggaaaag agaagagaaa tttaatatca tcataaaatt aaaaaataaa
     6661 ggatacaatt tgagtgaaat atgcgatatt atagatgatt tgaataaatc agaggttgaa
     6721 aaagtctata atcaaaacta atactatgat tgagatggca ctagataaat acaaagcttc
     6781 ctcttttaaa tttaaattaa tataaaactt tgctatttaa atatggtaga tataaagaga
     6841 tatataaata ataggtacaa agaggtgtat aaataaagga gataattcaa aataaaagtt
     6901 tgaaccatct ccttcttata gaaaaacaaa ggggttgatt tatatatgaa attttatact
     6961 atacagactt tagaattttg gaataacaat aaaaacaata aatatctatc aaatgattat
     7021 tcttttatat tagaagactt tgatattcca tacaaatgga tgtatagtca aatgacaaat
     7081 agaatatatg aatttgatga tagtatgata tgggtttgga cgaagagacc tgatttaaga
     7141 tgtagtggtt atttagaaaa aggaaaaaaa ggtgttttac ttgaaataga aataagtgaa
     7201 gacaatgtgt tattatcaga atttgattta tggcatttac ctttaatgga tattactgta
     7261 gagctttatg aagatgaaca aattgaaaag aaggtaagtt gggaaagaat atttgactta
     7321 gaactgtgtt caaaattaaa tgaagttaat atagaagatt tgacattgca aggagtgaca
     7381 tctaaagtgc ctctatctaa tgtaaaatta ataaaagaat ttgttgcaaa ataatatttt
     7441 tattgaaatt tattcaaaaa agttatatat ttataacaag aggtgttata aatacataac
     7501 tttttaaggg ggattttata tgtctaaaca acaaactggt attaaatcaa cacttatttc
     7561 agaaatattg ggaatttctt attgtattgg agctacatta gtaaataagt atttttttaa
     7621 aggaagtagt attgaatatc ttcctataat tatttgggta ttagctatgg gagctcagtg
     7681 ggtctataat aaaaagtatg atgatttaat tgatgaggca tcaaaatgta tactatcaag
     7741 agttaatgat attgcaataa aagtattgtt tttttctgta gctatagtat ctatatttct
     7801 tgtaactttg gacataaatg tgtcaaattt aaacatagga atatctttat tagtcatttt
     7861 gtttattcaa tccttgttga agttaattct atttatatat tttgacagaa aggggatata
     7921 caattaggtg tcagtattac taaagacaaa aattaaatcc tatagagaaa agaaccttat
     7981 gacacaaggt gaacttgcaa agtcagtagg agtaagacgt gaaactattg tacatttaga
     8041 aaatggtcgc tacaatcctt ctttaaaact ggcaatggat attgctaagg tatttgatac
     8101 aactgtagaa gagttgtttg aatttattga agaaaataat atttaaattt catgtattac
     8161 ttatgcatta gagaatgaat taatagataa aaaattcaag tttatgtatt agagaatgaa
     8221 ttaattatag gtaaaaaacc aagcttatag attagagaat aaatgactta tagatgaaaa
     8281 accaagctaa tggattaaag aatgaatgat ttatagatga aaaagaaagc ttatagatta
     8341 gagaatgaat taattatagg taaaaaccaa gcttatagat taaaaaatga atgacttatt
     8401 tatagatgaa gaactaaact tatagattaa aaaataaatg acttatagat gaaaaatcaa
     8461 gctcatagat taaagaataa ataacttatt agatgaaaaa acaagcttat agataagaag
     8521 tccatgctta taagccagaa aatgaatact ttaatagatt aaaaaataca tatttgtgaa
     8581 ttatagaatg aatgactcca taaactagaa aatttataat aatatctata tgaaaattag
     8641 aattatttta tcacaaaaaa attaagttaa ataaatatga aattacttcc aaatatactt
     8701 ccaaaaacat gtaatcgcat atataatata attataaaaa taataaatat ttttatagta
     8761 gtagttaatt attaataagg aggggtcaaa atgcctagca tgattatgga agttgtagga
     8821 gtactattta tagctattgg attaatcttg acattcattg aaataaaagg aaatttccct
     8881 tctttttatg gttttggtca aaattctata gggaaaaatg ctgtaggtat tatagcagca
     8941 ggaattgctt taattttctt tagaaatggc atagatagca tagattctat agctacttat
     9001 ggtgtatttt tagcaataga acttgttgta atagttgcat atttcttaat aagaaaaaaa
     9061 tttactaaag aagataaaca tttaagaact aattaactaa attatctact ataaaattgg
     9121 aaaattaaaa acaaataaaa gtttgttttt aattatttaa aatccaaatt tgatt
//
LOCUS       DAELJO010000154         9052 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAELJO010000154 DAELJO010000000
VERSION     DAELJO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.155"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(201..344)
                     /gene="scfA"
                     /locus_tag="KPB88_002948"
     CDS             complement(201..344)
                     /gene="scfA"
                     /locus_tag="KPB88_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG0055211.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(410..772)
                     /locus_tag="KPB88_002949"
     CDS             complement(410..772)
                     /locus_tag="KPB88_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG0055212.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(830..1123)
                     /gene="yajC"
                     /locus_tag="KPB88_002950"
     CDS             complement(830..1123)
                     /gene="yajC"
                     /locus_tag="KPB88_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG0055213.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1257..2378)
                     /gene="tgt"
                     /locus_tag="KPB88_002951"
     CDS             complement(1257..2378)
                     /gene="tgt"
                     /locus_tag="KPB88_002951"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG0055214.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2457..2942)
                     /locus_tag="KPB88_002952"
     CDS             complement(2457..2942)
                     /locus_tag="KPB88_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055215.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2954..3979)
                     /gene="queA"
                     /locus_tag="KPB88_002953"
     CDS             complement(2954..3979)
                     /gene="queA"
                     /locus_tag="KPB88_002953"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG0055216.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4297..5316)
                     /gene="ruvB"
                     /locus_tag="KPB88_002954"
     CDS             complement(4297..5316)
                     /gene="ruvB"
                     /locus_tag="KPB88_002954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG0055217.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5423..6025)
                     /gene="ruvA"
                     /locus_tag="KPB88_002955"
     CDS             complement(5423..6025)
                     /gene="ruvA"
                     /locus_tag="KPB88_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG0055218.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6018..6527)
                     /gene="ruvC"
                     /locus_tag="KPB88_002956"
     CDS             complement(6018..6527)
                     /gene="ruvC"
                     /locus_tag="KPB88_002956"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG0055219.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6854..7483)
                     /locus_tag="KPB88_002957"
     CDS             complement(6854..7483)
                     /locus_tag="KPB88_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055220.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7686..8009
                     /locus_tag="KPB88_002958"
     CDS             7686..8009
                     /locus_tag="KPB88_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0055221.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8277..>9052
                     /locus_tag="KPB88_002959"
     CDS             8277..>9052
                     /locus_tag="KPB88_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055222.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFP"
BASE COUNT         2878 a         1592 c         1038 g         3544 t
ORIGIN      
        1 tataagagta gctactttaa aataaatgct cttatatgat tagatatttt tgcataatta
       61 aacgataaga aatcaaattt aattttacat aaatagaata tatattttta tataataatt
      121 gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac cctcttacaa
      181 acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt acaagaagtt
      241 ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc agcactttgc
      301 tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa cgcctccatt
      361 tctaattgtt tcctattgat tatatcataa aactacttta atataaactc tatttaaagt
      421 tgacacccaa tacgcctcca atgcctcctg ctgctgatat taacaataac ttataaatca
      481 tccctacttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa atacatacta
      541 tgtacaaaag tcctactaaa agcccataca taagtccttt ttctttcatg tgttttgaag
      601 tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc atagttatat
      661 tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa acagccaaag
      721 ttattatata tgcatatcct agacctttaa aaatataatt agattttccc ataaaaaaac
      781 ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt tagtcttctt
      841 ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat ggaactttag
      901 ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct acattagcta
      961 ctattccacc tattgtaatg accttgtctc ctacacttag gctacttctc atttctttta
     1021 attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt gcgattacta
     1081 ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt tcactccttt
     1141 gtaaattata ctcttttgta aatcataata aatgtttata taacaattta ctttctctat
     1201 ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg caaaacctat
     1261 atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct atcttccatt
     1321 atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg agtagttatt
     1381 aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc tcttgaatag
     1441 tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc agcataagtt
     1501 gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct agcgatacga
     1561 gtaggtagga cacaatcaaa catgtctact cctcttataa caccttctac taagtcatct
     1621 ggactaccaa ctcccattag gtatcttggt ttatcctttg gcataagtgg agttgtatgc
     1681 tctaacacgt catacataag tggttttggt tctccaacac taagtccacc aatagcatat
     1741 cctggtaaat caatattagt tatttctttc gctgattgtt cccttaagtc tttatacatc
     1801 ccaccttgta ttataccaaa taaagcttgc ttgtcggtat tattatgggc atctttacat
     1861 ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct atctgatggg
     1921 tatggtgcac actcatcaaa tgccatcatt atgtctgaac ccaatgcatt ttgtatttcc
     1981 atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct aaattctaca
     2041 ccttcttctt ttatctttct taaaggccct agactaaata cctggaaccc tccactatct
     2101 gtaagtattg gtttatccca attcataaac ttatgaagac caccagctct ttttataagt
     2161 tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg tgaaccaatc
     2221 tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac tggcatgaat
     2281 attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc accactctgc
     2341 ttacaagtct ttatcaattc gtatcttact gcgtacataa agttctcctt aatcatattt
     2401 atttctcatt atttcttaag atatatttgt aataagctat tcatataatt tattgtctaa
     2461 atagatttat cttcgtcttc tttgttctgt tgttccttaa ttgcttcttc atcatgaata
     2521 ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat agcaaatagt
     2581 cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt aggcagtagc
     2641 aacatttttc tagcaattgc gtaaagtaat gcctcaataa atgctcctgg tatatgaaga
     2701 gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc taaaaaatca
     2761 ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc ccacataagt
     2821 cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc taaaacacgt
     2881 tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg taaactatgc
     2941 atctattacc tccctacttt ataatcatag catctccaaa actgaagaat ctatatcttt
     3001 cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata gcacttacta
     3061 acattattag tgtagactct ggtaagtgaa aattagtaat aagtttatct aaaactttaa
     3121 atttataacc tggatatata aatatttctg tccatccact agtttctttc atctttccat
     3181 cctcattaca agctgattct atagttctac aactagtagt accaacacat attacatttt
     3241 ttccattttc ctttgctcta tttattttgt cagctgcttc ttggctaacc atataatatt
     3301 ctgaatgcat cttgtgattt aaaacatcct ctacttttac tggtctaaaa gttcctaagc
     3361 caacatgtag agttacaaag gctatatcta cacctttttc ttttatttta tttaacaatt
     3421 cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc tttgaataaa
     3481 cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt agtggcatat
     3541 ttccaagttc atccaatatt tcttcaaaaa ttccatcata atggaatttt ataattcttg
     3601 aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca aaagaaaatt
     3661 tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta tcttcctctg
     3721 ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct cctattaatc
     3781 tagctggtat aactctagta ttatttaata ctaagcaatc tcctggattt aaatattcta
     3841 ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat accatcaatc
     3901 ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt aagtcaaatt
     3961 taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag ctacaataat
     4021 atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa ttgctatttt
     4081 taacagacac catatccaca atatatagat atttttccac gatatacatg ccttatccac
     4141 aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc tacagatata
     4201 attaccattt tgtggatatg tttgaatttt tagccatttt agaaatattt taatttaatg
     4261 actcttttat tttataaata ctcgtataat ctatttttat ttttcattgg gatatggaat
     4321 ttttaagtgt tcatatgcta atggcatagc tactcttcct ctaggacctc tatttataaa
     4381 tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat cttctccgat
     4441 agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta ttatagtcat
     4501 aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa gttccagagc
     4561 atcttttgcc actttatcag ttatttttcc atcagctctg acttgtgcaa aatctctaac
     4621 tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg caagttctaa
     4681 tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta ctatttttga
     4741 aagttcatca actgtataat agtccaattt acaaataaca ccaaatctat ctctaagtgg
     4801 atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag gaaggtcaag
     4861 tcttatgctt ctagcagatg gccctttccc tattattata tctaggcaaa aatcctccat
     4921 tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat ctataaataa
     4981 aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc tttctattgc
     5041 aggtcctgac gtaattctta aatttacccc catctcattg gctattatac ttgctagtgt
     5101 agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct catttctcga
     5161 tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac ccaaataatc
     5221 ttctaaagtt tttggtctta aactgttttc tatatctata tcttccatct tcattgtaga
     5281 agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc ttgtactttt
     5341 gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg ttttaaaatc
     5401 caaatattat aaaatccaaa tactatttca aggatttact cattatatat gtaagtgctt
     5461 tctttataat agcttctgta ttcataccat cttttttaca tttttgaact gcctcttttg
     5521 attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct tgtgatatca
     5581 aggtaggttt ttgtgataat aacgttggtt caaactctat gttatttttg tctaccttat
     5641 ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga gctttggata
     5701 attttccaat gtcttcactt aaaatatatg ctcctaactg tgctggtgat gcaaatgata
     5761 gtattgaaag cgccacctta ggtcctattt tagatacaga tgttagtaat tcaaacatct
     5821 taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt acaattaatt
     5881 tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg gatgatacat
     5941 ttattttata tcctatccca ttattttcaa caactatact gtctatataa atttcttcta
     6001 ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt tttttcaagt
     6061 ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc atctggtttt
     6121 ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc cttatctgct
     6181 ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata tacaggcttc
     6241 ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt tatagctgtt
     6301 tttacattct tgttaaagaa taattcttcc attccaactt catctatatt gtaattcttt
     6361 actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt catatgggca
     6421 ggtgttgtaa ctgctccata atcgattgct ttaaacttgc tatttttgta ttcaattata
     6481 ccatatccaa ctatagctat acctgggtca atccccaata ttatcatttt ttaaaattcc
     6541 ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt tctaaatcat
     6601 tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata aaatcaaata
     6661 aaaaagttat atcaaagcaa gattttacct caatataact tttatattta ttacaaataa
     6721 attattgtat ttttatagga attaaatgtg actacaattt taatactaat ctatttaata
     6781 ctaatctatt taatattaaa ttatttatta ttaatttact tagtatttaa cttaaaataa
     6841 tattaattta ctattaactt gcaaagtctt ctaatcttga ataggcctct tcttcagtat
     6901 ccataactac accttttttg atttcatctt gaactgtttt aggaagtgaa tctactctta
     6961 tttttgtttt ttctactaat gttttgcttc cactatcatc atatttatat agtgctatat
     7021 ttccattatc attttcaata gtatatttac cttttatagt aacatccatt gcttgtacat
     7081 ctttattatc actatattta gattctacca atatgatttc atttttatcc atactcttta
     7141 tttttttagt tggatattct tttttaaaat atgatactat ttctgacttc gtcttatcta
     7201 ataatgactc tggaactatt cctgccattg taagtgttct cttatagtta gtttcatttt
     7261 cgccttcact ttgaagccaa acttcacaat ttttatttaa tccaaaagct tcttttgact
     7321 tttcattcat attcgcacta gtacttacaa cttcaccagt ttgaactcca ttgtgtttta
     7381 atcttgtccc tatataaact ccgctactta atacaaatac tccaaccagt attaaggcag
     7441 atattttcca aggaaataat tcttttttct tcttttcaaa cattgattac gcctcctttt
     7501 tctattattt tctccaagct gtaagttttt atacaattta ggtaaatttt agaagaaaat
     7561 atatttatat ttcaaattac tctaagaata gatattttct aaaaggtagt tttctcttaa
     7621 tggttatatt cttgtaatta tatggacata atttataata aataatcgtt taggaggtaa
     7681 taactttgga aagaataaac tgtaatgtaa ttaactgttc acataacaac agccatatat
     7741 gttatgcaga taaaattttt ataaatggtg aaaaagctca caatgacaga catacttgtt
     7801 gtagttcatt tttagatgaa tctatttata gtaatttatc tagcaacgtt aataatagaa
     7861 attgtgagga attagtttgt actgtaaaaa attgtaaata caacaaagat ggtagcttat
     7921 gtacacttga taatatattg gttgctcctg aaactgatag agtaaacctt tatattgaaa
     7981 catgttgctc tagtttcgaa tgtaaatagt catagtataa atataagggt gtcaaatgta
     8041 attaaactaa catagtgtag agtatctaca actattagtt tgattatact tgacaccctt
     8101 atttatttta aataaaattt cattattata accatgttta taatgttcta actgtaatta
     8161 aatttgataa atttctataa attggtataa tcaatatgta aattgaattt agtaaattaa
     8221 tcataaaaat aaaatatcga gtatatagtg ttatactaac tagaaatgag gtgcttatga
     8281 gtacatttta tattcaattt ctttattttg taatttatag ctttatagga tggtgttgtg
     8341 aaactacata ttgttcagtt ttacaaaaac aatttgtaaa tagaggtttt ttaaatggtc
     8401 cattttgccc tatatatgga tttggagcat tagcaatagt tgctaccttg actccttttg
     8461 tacacactat tcctttgttg tttttgttta gtataattat aactagcata atggagtatt
     8521 gtacgagctt tattttagaa aaaatattta atatgacttg gtgggattat tctaaacata
     8581 aatttaatat tcacggaaga gtttgtctgg aaaactcact tatgtttggt gtattatcat
     8641 tgattgtaat gcttatagta catcctattg tggttgactt tattaactct atctctaaaa
     8701 atgtgctatt tatatttgct atatctatag aaatttattt tgttttagat ttagttatca
     8761 cagtccatac aattttacaa ttaaatggta agttaaaaca aatcaatctt ataattaagg
     8821 aattaaaaga taagaaagaa tactacaaac ttattacaca agaaactata gaaaacaaac
     8881 ttgaatcttt agttgaaaat agactggata ctttggaaga taggtttgaa atacttaagg
     8941 aaaaagaaaa ttatcgctat gctaaaaatc gtattgatga gctaaaaaat aaacttaata
     9001 ctttgctgtc taaccacaaa ttacttcata gacgtataat caaagcattc cc
//
LOCUS       DAELJO010000155         8974 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELJO010000155 DAELJO010000000
VERSION     DAELJO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.156"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            155..2221
                     /locus_tag="KPB88_002960"
     CDS             155..2221
                     /locus_tag="KPB88_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0055223.1"
                     /translation="MKEVVGNLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLHIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVIQTGSVDFIDVKDGNIVCVCCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQRLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            2238..4193
                     /locus_tag="KPB88_002961"
     CDS             2238..4193
                     /locus_tag="KPB88_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0055224.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            4209..5399
                     /locus_tag="KPB88_002962"
     CDS             4209..5399
                     /locus_tag="KPB88_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0055225.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6247..6430)
                     /locus_tag="KPB88_002963"
                     /pseudo
     CDS             complement(6247..6430)
                     /locus_tag="KPB88_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6547..6921
                     /locus_tag="KPB88_002964"
     CDS             6547..6921
                     /locus_tag="KPB88_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055226.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            6923..7780
                     /locus_tag="KPB88_002965"
     CDS             6923..7780
                     /locus_tag="KPB88_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055227.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQRIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            7783..8421
                     /locus_tag="KPB88_002966"
     CDS             7783..8421
                     /locus_tag="KPB88_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0055228.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         3255 a         1060 c         1630 g         3029 t
ORIGIN      
        1 aattaaatat agattaagta tagattaagt atgaattaaa tatactttaa attacaaata
       61 ttacaaggaa gccagaaggc ttcctaaaaa aataaccttt ataaaatttt ttattttaaa
      121 tttaaaattt tttattttaa atttaaaata aaaaatgaaa gaggtggtag ggaatttgaa
      181 aaaaattact aaagaaagga tagaaaatac tatgaaaaag ctatttacga gtgaaacatt
      241 tttggaatat caatttataa gtgattgcca aatatcacca aatggaggat atacagcatt
      301 tattgtaaaa aaagctgata tcaaagagaa tggttacaca agccaagttt atatattaaa
      361 caataaaact ggcgaattaa aacaaataac gtctataaac tctgttggag catatgcctg
      421 ggaagatgaa aatactatct tgttccctgc tttgagaaat gaaaaagtaa aagaagctgt
      481 caaaaacggt aagcaatgca tgagttacta tgctttatct ttaaatggtg gagaagcaga
      541 agaacttttt agactccata tcaaaggtgg taaattgaat cctataggaa aaggtttgta
      601 tgcagttatt gattcttatg acaatgaccg acctattgta gagggattgc cagaagaaga
      661 acaacagaaa aaaataaatg cttataacaa aagacattat gaacatttca aagaaattcc
      721 ttatgctgtc aatggggagg gatatattag cagaaaaaga aaacgcctat atatatacga
      781 cagtaatata aatgagttga aggcaattac agcacctatg tttaatgttg taggtatgaa
      841 aataagtgat ggaaaaatct tatatatagg gcaagagttt aaagatgtaa aaggtttgaa
      901 aaatggggtc tatgtatttg atacaaacac aaacacaaat gtctgtatat tagaaaaaga
      961 taagtatatt ataaaaggtt ttgaattata tcaaaatcaa gtgatactaa acctaactga
     1021 tgctttatct tatggaaatg gagaaaatgg agatttttat actattgata ttgatacgaa
     1081 agaaatgaaa cttctttcag ggcatcaaca tcattgtatt ggaaatacag taacatctga
     1141 tgttaaaatg ggtgctggtc agacaacaaa ggtagatgga gaatatattt attacacttc
     1201 tacagtagat atggactgca ttattgaaag aatacatatt ccaacaggaa aacaggaaaa
     1261 agtaatacag acaggttcag ttgattttat agatgtaaaa gatggcaata ttgtatgtgt
     1321 gtgttgttta ggaaatggat taccagaagt ttatacagta gagaatggaa cattgtgtaa
     1381 gaaaacacat ttaaatgacc atattttaga ggaatataaa atttctgtgc cagaatacat
     1441 agagtcaaaa ggaagttcta aatgggaaat acaaggatac gtatataaac cagtagatta
     1501 tgaggtgggg aaaaaatatc cagctgtatt ggcgattcat ggaggaccaa gattgacata
     1561 tggaccatat tttatgcatg agatacaggt atttacaagt gcaggttact ttgtattctt
     1621 ctgtaaccca agggggtctg aaggtcgtgg caatgctttt gcagatatta ggaaacagtt
     1681 tggagaaatt gattatatag attttatgga atttacagat actgtacttg aaaaatatac
     1741 agacattgat aaaacaaaat tggctgttga aggtggaagt tatggtggat ttatgacgaa
     1801 ttggataatt ggtcatacaa accgttttgc tgttgcttgt gctcagcgtt ctattgcaaa
     1861 ctggagtggt atggagggta caacagacat tggctattat ttctgcaaag gacaaacagg
     1921 tgcttctcat atggaaaacc acgagcttca atggaaacag tcacctcttg cttatgctga
     1981 taaatgtgtt acaccgactt tatttttaca tggtgaaaaa gactatagat gctatatgca
     2041 agaagcattc cagatgtttt cagcattgaa aattcatggg tgtcctacaa aactttgtct
     2101 atttgaagga gaaaaccatg aactgtctcg ttctggtaga ccaaaacaaa ggctacaaag
     2161 attagttgaa atgctagatt ggttttcggt atatataaaa aaagataaca taggacagta
     2221 gaaaggagaa tataaaaatg gaaaaaataa agattgaaag ttcagatatt tttgaatata
     2281 tattccctca tgatatttct tgttcacctg atggaaagca tattgcttat attatatcaa
     2341 atatcaacga ggaaaaagac tgctatgaac atgacttata tgtgatggac atcaaaacag
     2401 aaaaacaaat acatatgact caaacaaaag atgtaacatc atttagttgg atttctaata
     2461 cagaactact ttttacatct aaaagaaata agccaaaagc aggtacaaca gatttttaca
     2521 ctatttctat agaaggagga gaagcaaaaa aagcattttc tattccaaaa gcttgtagcg
     2581 tacctgtatc acttggaaat aaattgtggt tactgacaac aaaaaatcca acggattcta
     2641 aaaaatcaga accagatagg gcagtagagg gtgtagatta ttggacattt acagataaac
     2701 cttttataag agatggagaa aatttttctc aaagaagacg tgtaacatta gaattatatc
     2761 aagaaggtga aaatataaca aaagccatta caccaaaatt ttgtgaagta gcaggtgtag
     2821 acgtttcttc tgataaaaac agaattttat atactggtca gatttatgaa gattgtgcaa
     2881 ctcctttttc tggactttgg gagtatcata tagacagtgg tgaaaccaaa gaacttgtac
     2941 cacaaggaaa gtatcaaata agtttggcaa aatatattgg caaagataaa gtaatgttgc
     3001 aagcttcaac attagaccgt tctatcacac agaatcatga tatttttata cttgatttga
     3061 gtactaatga aataaatatg attgctagtc cagatggtat gtatgctaca ttgctggatg
     3121 tagatgcagt atatggtggt ggacgtagta ataaagttat aggagacaaa tttattggtg
     3181 caagaatttg cagaactatg acagaattta atgagtttga tacaaagaca ggtaatatac
     3241 gtatcatcac aaaagtagat gcttttacat cttttgatat ttacgacaac accatgtaca
     3301 cagtaatgtt gaaagattat gagttagcag aaatatatag cattgatatg actacaggta
     3361 caatgaaaaa aatgactgct ttttctaaac catatttaga tactcataag gtttcattac
     3421 ctgaaaaatt gacgtttgta gcaaaaaaca aagaagaagt tgatggtttt gttataccac
     3481 ctatagatgc taaagaaggc gaaaaatatc ctgctgtatt gttcattcat ggtgggccta
     3541 aatgggcata tggctatatg tttacacact tgaagcagtg tgtaacatca aagggaatgt
     3601 atgtgattta ttgtaaccct catggtggtg acggttatgg tgaaaaattc ttagaaatgg
     3661 tagaaagatg ggggtatgtt gactatgagc atcttatgga atttgtagat acttgtattg
     3721 aaaaatatcc tgggattgat gcagaccgct taggggttgc agggggaagt tacggtggtt
     3781 atatgacaaa ctggataatt ggtcatacag accgtttcaa agcagcagct tctcaaagag
     3841 gtattagtaa cttgattaca gcttctttaa ttattgactt tggggataga attatgaagc
     3901 agacttgtgg agataagacg ccttggaatc atgaggaggt tctttggaat cattcaccta
     3961 ttaaatatgt gaaaaatgtt aagacaccaa cattgttctt acatagtgat agggattata
     4021 gatgttttat gggggatact ttccaaatgt ttacagcatt aaaacagctt ggtgtagata
     4081 cagaaatgta tttgttccac ggagatacac atggattgtc tagaaatgga agaccatcta
     4141 atagaattgc aagagcaaat gctattgtag attggtttga aagatattta taataagggg
     4201 gttattctat gagtgatatg ttattatggt ttagtgtcgt attagtttta tttgctattg
     4261 gggatttaat tgcaagcaaa acaaaagcaa aagtatcagc tgtatttgtt acactacttt
     4321 tatttttgat tctatttgtc acaaaagcaa taccagctga tattattgaa aaagcaggta
     4381 tgaccgcagc agctagttgg agtgttccaa tgattatgtt tagtatggga acaatgttga
     4441 atgttaaaca atttatagac gaatggagaa cagtattgac agcatggctt ggaattgtag
     4501 cagttatagt ctgtgtctca ttatgtattc ctttgtttgg aaaatcaaca gtattgacaa
     4561 gcataccagt tataaatgga gctttacctg ctactactat tatgacacaa gcagcactag
     4621 aaaaaggact aacacttgca gcagctacag caacagttgt atttgcaatt cagaagttta
     4681 ttggtacacc aatagcttca agagcagcgt tacaagaggc aaaccgtctt ttagtagaat
     4741 atcatgaagc aaaatcaaaa ggcattgatt tagcaaatgt tgatactgat aagaaagaag
     4801 cagagaatac aggcacaaaa gtaaaacaag ctttctgtga aaaatacgat aaatattatt
     4861 ctacaaatgt ttgtatattt tttatagcat tattctctta tctagggtat gaactttcag
     4921 agattattca tgtaaattat tctattgtat gtcttgttgt tggtgttatt gtaacacgta
     4981 ttggtattgt accaaaagac attttggaaa aaggaaaaat caagggattt atcaacatgg
     5041 ttgtgtttgc agctgttatt ccgtctttag caaaagtttc tttgacagat ttaattagtc
     5101 tttttgtacc aattgtgggt atgtttgcag cttctatcat tggtatattc cttatgatga
     5161 aggtgttacc aggttggaaa attatcggtt ctaaacctct tgcttttgga gtagggtttt
     5221 gtcagatgtt gggattccca actacatatt taatttcaaa tgaagtgtgt aatgctgttg
     5281 gagaaacgga agaagaaaga gcatatttga tgtctaagat tatgcctaag ctggtagttg
     5341 gtggtatggc ttgtatgatt tctattgtgg tagctggtct tatggtacca atgttgtaga
     5401 taggtttcaa agttaaaaaa gtggtgttaa tttataggag aagctaaaaa gtagtattaa
     5461 tttataaaat aattttatat tagttaattt gttataagaa tattttcttt ttataattat
     5521 aattttgatt ttataagata gcagattttt tatttgctat gctaatagtt ataatttgtt
     5581 tttatttgct aaaatgcaac tttaatttga tttatgtata atatagggta tttaataata
     5641 tttttgaaga atttttataa aaaattaaaa agaggagaaa aaagggagtg ccacaaagtg
     5701 aactttttag ttctaaggca ctcttttttg tacgaaatcg aatatatttt caacgtttta
     5761 aaagtgagaa agagacttat ctctagtttg agatagccaa ttttaattat taaaattatt
     5821 ttttatttta tttatcaaag agtattcgtg ttttagaagt tagtattttt attttatata
     5881 tacaaggttt tccgtttcta atgttttctt ttttaaaata attagggtat tcaattatag
     5941 taaaaggtgg atggtatttt gcagtgatat tatttaaagc acagtattga atgttgaaat
     6001 cctcacattt tagagtatta tcttttgtaa tcaaaaaaat cttctatact tatagcaagg
     6061 ttatttctgc aaatattcca tgcatcataa tatctaaatt taaatttttt taatatagag
     6121 tttacctcta gtctacactc ttgaataact ctttcctata tccaaattct tataaattca
     6181 tcccatttat tactatctat ttattactat ctaaattttt tagtatgttt gtaattagaa
     6241 atggatttat tcctttatgt tttttataaa tagtttataa cttaatttat cattaaacaa
     6301 atcattcata caggattact ttttctttta tatattcaat tcttactttt aataaatatt
     6361 taatacatcc tatcttatgt ggttttaaaa agtgtttata gctatcaaca atctctaata
     6421 tttcattcat atttattttg tcatagtaat attttacatc ttttatattg acaaaaaagt
     6481 gctctaagtg tatagtgtgt atataacaac atcacactaa aaatgataaa aagggggtga
     6541 taaaaaatga aagtgctcat ttctaacaca tcagataagc cattatacca acaaattaaa
     6601 gagcaaatta aagaagcaat atttactgaa gaactgaaag aaggggatgc aatgccctcc
     6661 ataagaaatt ttgctaatga tttaaaagtt agtattttga ctattagacg agtgtatgaa
     6721 gaacttgaaa atgaaggctt tcttacacgc caagttggaa taggaacatt tatttcaact
     6781 ggaaatttag aaatactaag agactcaaag agacgtatag ttgaacaaaa aatggcagat
     6841 atgattaagg atgcaaaagc actaggaatt acacagatag agttgcaaga aatgatggat
     6901 attctatatc aggaggatta atatggaaaa tattttagaa gtttataatt taaataagac
     6961 ttataccgat ttttcattat ctaatataag ctttgcatta ccagaagatt gtataactgg
     7021 atttataggt gttaatggag caggaaaaac aacaacaatc cgttcgatat tggatgtcgt
     7081 aaagcgtgat agtggaaaaa taaaaatctt tggtaaagat ttcgatggaa atgagaagga
     7141 attaaaaaat cgaattggag ttgtatttga tgattcatat ttctatgagg agttgtcaat
     7201 ggcagaaatg aaaagtatta ttgcaccttc atatgataat tggtgtgaag aagattatca
     7261 acaaaatatg aagcgtttta atcttaaccc aaaacaaagg attgatacac tttcaaaagg
     7321 aatgaaaatg aaatttgctc tagcattagc attatcgcat aaagctgagt ttttaattat
     7381 ggatgagcct acaagtggtt tagacccact aatccgtagt cagatacttg atatactaac
     7441 agaatatatg aagaaatgtg gtcgtggtgt tttcttttcc actcatataa catctgattt
     7501 agacaaaata gcagatatga ttgttatgat tgacaatgga cgtattatat tagagcgtga
     7561 aaaagatact ctattggacg aacatagaaa agtaaaaggg gatattcaat ttctaaatga
     7621 tgaaaccaaa aaattatttt taaatttaga aattacaggc tatggtttta tagggattac
     7681 ctcaaaaatt aatgaagtgc aaaaaaatat gccagatatt ttaatagagc gtcctacaat
     7741 tgaagatatt atgcttgcat atattgaaag gagagagtaa ttatgttgat tcatttagtt
     7801 aaaaaagata ttctgattgt aaaaaaatat gtattgttga tattgtttat tgcaatagga
     7861 atcccattat ttatattgtg gcgtttacct ccatttgcac aaatattggg atttgtacta
     7921 tcaattatat tttcagaatt tatgctgtgc cagtatctat tattaaagga gagtcaatat
     7981 tcaaaagcat ctgtattact ttgttcaact ccttatccta gacgtggctt agttcaatct
     8041 aagtatgttt tatttgcaat ggtatttatc tattgtacat tagtttactg gattgaaaat
     8101 ttacttattc cacaaatagg aacttttgac ttgacatata ttttgattgt tttattaata
     8161 agttctatta tttatggagt ttatatgcct attcaataca aattaggata tgaaacaaca
     8221 aaattctttt tcgtgatgat aatcatggct tcatcatttg tattaccatt aactatagct
     8281 cacagcaatt ttgaaattcc atttatatat cagtatccat ccatgatagt gaatattgta
     8341 ttgttccttt taagtttaat tatactgtta gcttcaatgt ttatttctat taaaattttt
     8401 agtaataagg agcttgtata aggtaagggg gacttttata tgccacattt accagattgg
     8461 gctgttttac tttgtatctt acttccaata gtatataaag cttgtcagat atttaaaagt
     8521 aaaaaataga gtatatattt atattgccat ttttattgag tgaagttaga tggtatgaaa
     8581 aggttacttg aaaataaagt tacttggtgt gggagagtaa catcataata ttatagatta
     8641 tatgtttctt gtgaatcaat ttgtattttt taatagggaa tatccagtaa ttttgttttc
     8701 cattgattat ttttcaatta tgagtatgat tgtgtgtatt gtataccatc ttttaaggtg
     8761 tttttaaaaa attatatcgt caaaatataa atctaaggaa tcctataaat aaagcttttc
     8821 aattttagaa aactaataaa aagtaggaag ttaataaaaa gaagctgtct caaaatagag
     8881 ataagcttct ttttcattgc tgaaatgacg aaaatataat tgatttaata taaaaaagag
     8941 tgctttatga actatttatt ttgaacagta gata
//
LOCUS       DAELJO010000156         8764 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELJO010000156 DAELJO010000000
VERSION     DAELJO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.157"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            194..364
                     /locus_tag="KPB88_002967"
     CDS             194..364
                     /locus_tag="KPB88_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055229.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(540..1079)
                     /locus_tag="KPB88_002968"
     CDS             complement(540..1079)
                     /locus_tag="KPB88_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0055230.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1218..1850)
                     /locus_tag="KPB88_002969"
     CDS             complement(1218..1850)
                     /locus_tag="KPB88_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG0055231.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1986..3899
                     /locus_tag="KPB88_002970"
     CDS             1986..3899
                     /locus_tag="KPB88_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0055232.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3915..5291
                     /locus_tag="KPB88_002971"
     CDS             3915..5291
                     /locus_tag="KPB88_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0055233.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5431..6081)
                     /locus_tag="KPB88_002972"
     CDS             complement(5431..6081)
                     /locus_tag="KPB88_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG0055234.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6552..8564
                     /locus_tag="KPB88_002973"
     CDS             6552..8564
                     /locus_tag="KPB88_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0055235.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DLINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         3317 a         1105 c         1209 g         3133 t
ORIGIN      
        1 aatttaattt tatttttaaa ataaacttct ttaaaataaa taaaaatata agcagattaa
       61 actttattgt aaaactttaa aacaagaata tcataataaa gtttttgttg tagaattaat
      121 ttaattgtta tattcaataa tatttgttat aatgttattg aattacaata catataaaag
      181 ggaggatttc aaaatggcat ataaaattac tgacgcttgt ataagctgtg gtgcttgtga
      241 ggctgaatgc cctgttagct gtatatcagc tggagacgac gcatatgtta tagatgctgg
      301 ttcttgtata gactgtggtt cttgtgcagg agcttgtcct gtagatgcac ctcaaccaga
      361 ataataattc gttattcata aagagactga ttttatttta taaatactaa tcagcctctt
      421 tttaattatg tatgattcaa ttaatattat ttatatactt tattatgttg ctacctaatt
      481 catcttaaaa tactgttagc acatatctaa tatttttaac tttaatctaa tttataattt
      541 taatactcct cataaaatct ttcatattta ttaaagcaat caagacatac tttctttcct
      601 tcttcaatcc ttatatattg ctcagatgca aattcaccac acacttcaca tttaagagat
      661 ttaaatattc ttgctttttc aggaagtctt aacttagtat ctgtaatctc aaatatttct
      721 tcaaaatcac tttctaataa gtactttttg tatccttctc tatccatttg aaatggcttt
      781 gactttacat atattcttat agattttcca ttttccctat taaagaaatg atatactgat
      841 ttcccagtca tcctaagtag taaatttcct ttacccattg tgcaacttag cacgacttgt
      901 ataccatcta ttccacaagc atcattttca gaaatacata caatttcctc atctcctgac
      961 tgtttacatc ctaaatattc tcttgctgca agtgctgctt taaatcctat agctaatcct
     1021 ggacattcat gaccatgaaa ttcaattgcc tttttccata attcctccac aaaaaccact
     1081 ctccttaata ttgtttattt ccatacatta tttaaatttt tatcctaata tttcaaaaag
     1141 ccagaggatt taatattaaa tcttctgact ttctcaatac tctttacaac tgctcattat
     1201 taatatgttt aagttgtcta ctttccttct ttcattaaat acgataaggt aaataaactt
     1261 tctgttaagt ttacattttc aactataaca cctttcggaa cttctaaatc taaaggtgca
     1321 aaattccata cacctttgat tccagctttt acaactctat taataacttc ttgtgctcca
     1381 tttttaggta tacataatac agcaatatct atattattgt tttttataaa gtcttctaaa
     1441 gtatctgaat ctaatacttc aaactctctt atctttaaac ctatcattct aggatttgca
     1501 tcaaataaag cttttatctc gaatccagct tttctaaatc ctgcataatt ggctatagct
     1561 tgtcctaagt tacctgctcc tacaagaact gcgttgtatg gtcgatccaa cccaagaatt
     1621 ttacctatct cagtatgaag agcttctaca ttataaccat atccttgttg tccaaatcca
     1681 ccaaagttgt ttaaatcttg tcttatttga gaagcggtaa accctattat atcactcaat
     1741 tctttagaag atattctttg tatatcccta tctaataagt ctccaagata tctatgatat
     1801 tttgggagcc ttcttataac tgccattgat atatttttat ttcccaacat cttcacctcc
     1861 taaaagtgtc ctcttttttg tttgattcac aataaaatat ttctttttca aaactaatac
     1921 atataacctt gattatatta tatcaaaaca ttataatttt agatagttca aggaggtaga
     1981 aatttatgat tgttttgtca tgtaataact taaataaaag ttttggtata gattctatat
     2041 tagaaaatat tagttttaca gtaaatgaag gcgataaaat tggtataatc ggtgttaatg
     2101 gtactggaaa aactacttta tttaaaataa tttcaggaat ttatggatat gatagtggag
     2161 atatttacac ttctaaagat tgtgaaatcg gatatttaga acaaaatacc aatttttatt
     2221 cggataatac tattttggaa gaagtactag aagtttttaa aaatttgata gaaatggaat
     2281 cctatttaag agaattggag gttaaaatat ctgaagaaag tacaaaaaca aactctccaa
     2341 ttatagaaaa aatcatggat gaatattcac ataaactaga attattttca gatttaaatg
     2401 gatatggata taaatctgaa gccaaaggag ttctaaaggg attaggattt agtgacaatg
     2461 atatggataa gccaataagc atactttctg gtggtgaaaa aactagagtt cttttaggaa
     2521 aattacttct taagaaacca actctattgt tacttgatga acctactaac cacttagact
     2581 ctgaagcaat agaatggtta gaagtatttt taaaacaata taaagggact gtcatgttaa
     2641 tatctcatga tagatatttc ttagaccaat ccgtaaatag aatttttgaa gttcacaaca
     2701 aaaaacttaa agtatataat ggtaactact ctaaatttgt tgagttatct aaaatagaaa
     2761 aagaactaga gcttaagaaa tttgaagacc aacaaaaaga aataaaaaag caagaagaat
     2821 caattgaaag actaaaagct tatggtagag aaaaacacct aaagcgtgca agaagtaaag
     2881 aaaaagcatt agacaaggta gacgttttag ataaaccaga agcttatagg aaaaaagctc
     2941 gtatacaatt tacgccttct gtccagagtg gtaatgatgt tcttcaaatt agagatgtat
     3001 ctatgggtta tggagaaaga atacttttta aagacttgga tttagatata tatagagggg
     3061 aaaaagttgc acttataggt gcaaatggtg ttggcaaatc tacattattt aaaataataa
     3121 ctaatgaact acaacctctt agtggcaata ttaaatttgg aactaatgta catgtttcct
     3181 acttccatca ggaacaaaaa actcttaatt tagataacac aataattgat gagatttggg
     3241 aaaataatac tcatcttact caaactactt tgagaaatat gttaggggca tttttatttg
     3301 tagatgaaga agtttttaag aagatttcta cattgagtgg tggagaaaga gctagagttg
     3361 ccatacttaa acttatacta tcaaatgcaa acttattgtt attagatgag cctactaatc
     3421 acctagatat tgattctaag gaagtccttg aagaggcatt aactaattat gatgggacta
     3481 tatttacaat atcacatgat agatactttc taaatactgt tgttgataaa attttagttt
     3541 tagatgaaaa tggaattact gaatatctgg gaaattatga ttattatata gataaaaaaa
     3601 ggcaaatcca agagatgagt attatagaag aaaaagaaga aaaaactaga actcaaataa
     3661 aagatgaaaa aagaaaagag agagaacaaa gagaaataga aaagaaaaat agaataaaaa
     3721 gacaaaatat agaaaaagaa attgaaaagt tagagattga aattgaaaaa ttagatatac
     3781 ttttatgtca agaagaagta tactctaatc cagataaagc taaagaagtt agccaagaaa
     3841 aaattaattt agaaaataac cttgcctctc tatatgatga gtgggaagaa tttatgtaaa
     3901 aaggagagga ttatttgcaa gaactatttt ctttaaaaga cgaaaataaa agattttata
     3961 aaatacttct atctttatgt atacctatta tcatacagaa cttaatttct acttctgtaa
     4021 atgtaataga tacaattatg ataagtagtt tgggggaaac atctgtagct tcagttggag
     4081 ttgcaaacca atttttcttt ctttttaata tgtccttatc tggtatcaca ggaggtgctg
     4141 gagtttttat ttcacagttt tatggaaaga aggatgtgag taatataaga aaagtcacag
     4201 gtcttacttg tgtattggca attgttttaa gttttgtatt tgttattcct gctttactaa
     4261 caccaaagcc aattatacac atattttcgt atgattctga ggtggtaaaa ctttgtatag
     4321 attactttag tattgcagta tttagttatc cattaattgc tgtaagtact gtatttagta
     4381 ctggttcaag aggtgttaga aatcctaagt taggtatgat ttgtagtgca tttgcacttg
     4441 taacaaatgt tattttaaat tatggattta tatttggtaa cttcggtctt cctgcattgg
     4501 gtgtaaaagg tgctgctcta gctactgtta ttgctagaat atgtgaatta attttaatga
     4561 ttacttatgt ttacctttat aaaaaagact atatattaaa gtttggtcta aaaaatctaa
     4621 aagctattga taaaattttt ataaaatctt tttcatcaaa aagctttcct atatttgtaa
     4681 atgactctgt atgggcaatt ggaactgtcc tgtattctgt tgcatatgca agagctggta
     4741 cttctgctat agcagctagc cagatagcaa ccagtacagg taatttcttc ataatgacag
     4801 ctgtatgtat tgcatctggt gcatctatta tgcttggaaa tgaacttgga gctgaccata
     4861 ttaaaagagc cattgaatat gctaaaaagt tttctatact tgtattttca gctggtttaa
     4921 tacttggaat tattttaatt ttaaatatac cattattatt aaaaatgttt agtgtttctg
     4981 atagcttagc ttctgatata actaagatat tctttataat gggaatacta atggctctta
     5041 aatcttttaa tacattggtt ataattggta ttttaagaag tggaggagat actaaatatg
     5101 ctctatttct agaattagga tgtatgtggt tagcttctat tcctctaact tttatagctg
     5161 catttaaagg tgcacctata tttgtactcg ttttgctcac ttatagtgaa gaagtggtta
     5221 agtttatatt tggtgttcct agagcattat ctaaaaaatg ggctattaat atagtaaagg
     5281 aaattgatta ataattatac tatacattat aagtatacaa tatttctaaa cagtctcatt
     5341 gaaaataact aaagtatttt tgattttcag tgagactatt tctatttttt acagaaatta
     5401 ttatacttta ttttgattaa gtaacaattt ttactcatct tccaaaaatt cttttacttc
     5461 aaatgccaat ttatgtaatt catcatagtt taaaatttca tatgtattac ccattttttt
     5521 tactatctcc ttttcagtta actttagcag agaatggtat ctactttttc tactaattga
     5581 atagatttca ctgaaattac gaatagcttt tatattgcag acattatttt cttgtttatt
     5641 tgatatacaa taggcaataa cttctgtgca agaaaatagt tctcttacca ttctggtttt
     5701 atataattca tatattcttc ttgtacattt tctgtgaaat agtgcaataa aatctggttt
     5761 gagataaaac tcttcaaata catttttttc aaatcgaaat aattttatat tagtctttgc
     5821 aaatatatca catttcaaat tatgttcata tatgtaacta attttgccta taaattcatt
     5881 ttcaggtaca atgtcaacaa gaaatttatt tcctcctcta gtagtacact ccactaatgc
     5941 aactccctct attataaaga acacttcttg taagtcttta tctgatgcta caaatctttg
     6001 acccttttta tattcaacaa ttgatatttt gtttaaatta tttttaataa ttggatatat
     6061 acttgttttt tctgctttca tcaaagttac ctcctttaca atatattgta taaaatattt
     6121 tatttatatg ttttaattta tattattttt aataaaagat aaaattacaa ctaaattaaa
     6181 cttgacaaat tcattctact cacgcagaat aaacttatac atatatattt tcatatataa
     6241 tttattttaa tataattcta tatttacaaa attatattat caatagaatt tattaaaata
     6301 aatttttttg ggtgtatatt ataatttaat cgaatagaaa tgaaaaatat acatacagtt
     6361 taagttttaa gcaacaatta gggggatttg gtgacaagat ttttaattat aagaaagaaa
     6421 gtgtcttaat gcaactttta taaagtcatt atagacactt tcttttttat ttgataaatt
     6481 taaattatca aaattatatt gctttataca actattttgt tggaaactat ttattatata
     6541 aggagaaata aatgaaaaaa tcagtagctc tggtaaatga tagtagaaaa gatttaatag
     6601 actttttaga aaataatttg aaattagttt ttggagattc tataaatatt aatagatact
     6661 tcataaatga aataaatgat aatgatctta taaatgatga tgtgatatta gtaatgtcag
     6721 tagaaagatt agacaaaatt ataaataata tactagataa gaaaaaagtg attgtggtaa
     6781 gaagaacttt tagggaagat aaaatttata atctactatc tttaccacag ggtacaaacg
     6841 ttctaatcgt aaatgattct gatgaaacta ctctagaaac tataagttta ttttataaaa
     6901 ttggagtgac taatattaga ccaattccat atatgaatga taataactat aaaaatataa
     6961 aaatagctat aactccagga gttccagaaa aagtacctag ctttatctca gatatatttg
     7021 atttaggtca cagatatata gatatttcta catttataga aataataaat ttattacaaa
     7081 ttgattcaaa ggaaatacaa tctaatctag ttaaatactc agaagaaata ataagtttag
     7141 atactggtat aaaagataaa tataaggaat tatttttaaa aatagaagaa ttagatacaa
     7201 tattgaattt atcaaaagat ggtatattat ttacttcaaa agatggtgaa ataaatacat
     7261 ataatagtaa agtaaaagat attttagaca taaatgagga tatatatgga aaatacattg
     7321 aggatatatt tgtagatagt ttaaaagttt tattgagtga gaaagagata ctagataaag
     7381 ttgtcgtatt caataagaaa tatataaatg taaataaaaa aaacatatac aatagagatg
     7441 aaaaaatggg cacatattat agtttacaag aaataacata cataaaaaaa ttagaacaaa
     7501 atctcactaa aaaattaaga gaaaaagggc aaatagcaaa atatacattt aaggatataa
     7561 aaacaaatag tcctaaaatg tttgaatgta tagatttagc taagaaggtt tctaaatctg
     7621 acttaagcat acttatcaga ggtgaaagtg gaactggtaa agaactcata gctcagtcta
     7681 tacataacaa ctcaaataga aaaaatcaac cctttatagc agtaaattgt gcagccgttc
     7741 ctgaaaattt attagaaagc cagctattcg gatatgacaa gggaactttt actggaggtc
     7801 taaaagatgg aaaacaagga ttatttgaac ttgctaataa tggaacaata ttcttagatg
     7861 aaataggcga tatgccttta gaattacaaa caaaactact tagagttctt caagaaaagc
     7921 agataatgcc tgttgggtct cacaatgtaa ttaatataga tgttagaatc atatctgcta
     7981 caaataagaa tttagaacaa atgattgata atagccaatt tagagaggat ttatattatc
     8041 gattaaatac aattccaata aatatacctc ctctaagaga aagaaaagaa gacatattaa
     8101 ttattatgga agatttaatt aataagaaat tagtaatcac accagaagca aaaaaactta
     8161 tacaaaacta tatgtggaaa ggtaatataa gagaactcca aaatgttaca tcctatttaa
     8221 acattatgtg tgaagatatt gtattagaga aagatttacc tccaaactta aggtcttcag
     8281 ataacaaaaa tacatcatta aaactaaaat atagtaagaa tgatatattg aatatattag
     8341 agatactaat tttaaataag gaatcagatg ttggtattgg tagaggatta attttaaaag
     8401 cactactcga taagaacctt caaattacag aaggaaaaat taaaaaaata tttgaatatc
     8461 taaaaaaaga agaacttata atttgtagtt caggaagata tggaagtaaa ataactcaaa
     8521 aaggagaaga tttttacaat aaattaaaat ataaaggtct ataaaattat aatttatcaa
     8581 acaaagaaat ctattcagga ttaaagtact ccttcaatag gtttttttat ttttcaagta
     8641 ccctaaaatt ataatttatt ttgtacataa ataataggaa ttaataggaa taggataatt
     8701 tttttatttc ctattttttt tatataaact agcatataaa atatattttc ttcaaaataa
     8761 tatt
//
LOCUS       DAELJO010000157         8713 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELJO010000157 DAELJO010000000
VERSION     DAELJO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.158"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            32..1411
                     /locus_tag="KPB88_002974"
     CDS             32..1411
                     /locus_tag="KPB88_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055236.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            1652..2341
                     /locus_tag="KPB88_002975"
     CDS             1652..2341
                     /locus_tag="KPB88_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055237.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            2562..4934
                     /locus_tag="KPB88_002976"
     CDS             2562..4934
                     /locus_tag="KPB88_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG0055238.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            4949..5851
                     /locus_tag="KPB88_002977"
     CDS             4949..5851
                     /locus_tag="KPB88_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0055239.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFILFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            5871..6422
                     /locus_tag="KPB88_002978"
     CDS             5871..6422
                     /locus_tag="KPB88_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055240.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            6586..7458
                     /locus_tag="KPB88_002979"
     CDS             6586..7458
                     /locus_tag="KPB88_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0055241.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            7622..8602
                     /locus_tag="KPB88_002980"
     CDS             7622..8602
                     /locus_tag="KPB88_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055242.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
BASE COUNT         3529 a          817 c         1531 g         2836 t
ORIGIN      
        1 agaaattctt aaaatttctt ggtgatacaa tatgctaaac tttatgactg agctattagt
       61 agttttatct atatttatac agttttctat aatgaacgga attttaattt ataaaaagga
      121 tagtaaaaaa aatgaaaaaa tagtattcta tattgtcttg tttcttattt atattataat
      181 tcagataagt agattattta atggaggaaa ttactttagt aatataaact atttatttta
      241 tatattagca tttattgctt ggggaggata ctataattat ttttataaga tgggttatat
      301 taagtattcc attttctttt atctatttag tgtatattat aaatgtttgg atgctgttat
      361 atataggata ctattttttg caattacagg taatgttatt tcatctaaag aatctttatt
      421 agtacctcac ttagaatcta taaaaattca gatatatagt aatttaataa ttattcttat
      481 atatcttata tttagctttt ttagaaagtc tataaaatta attagccaag ataagagaaa
      541 ttatgtatat ttattatttg ccttattagt taatacaatg aatatgcttg taaattatgt
      601 attaaaaaaa ttagaacggt ttgggtcatt acattcagaa ggatactatt ttgataattt
      661 tgttaatcca aaattggtag gagctagtag tatatttctt atactattgt ttaaagaaat
      721 aataaaagag aatagattaa aatctcaagc tgaattaata aaaaataagt tagatatgca
      781 atatgcgcat tatttaagta ttcaagaatc tcatatgcga gttaagaaac tatatcacga
      841 tataaataat cacatttatt gtatagacaa ccttagaaac aatagcaaag aaattaatga
      901 atatgttaac aatttaaaag atgaaataaa aacatttaaa tatatctata atacaggaaa
      961 tatgatatta gacattataa tcaacgaaaa gagtgaaata tgtttaaaaa aaggaattaa
     1021 atttacttgc tctataaatt tttcaaaagt aaattttgta aaacctatag atgtgtcaag
     1081 catattttct aatattttgg ataatgcaat agaagcatgt gataaaatag tggatgaaaa
     1141 tattaataaa tatattagaa ttaaaggaac tataacaagg tcattttttg tacttaaatg
     1201 tgaaaatagt aaactaaatc agcttacttt taataaaaat atattgttaa caaacaaaat
     1261 ggataagttc gtacatggaa taggtattca gagtattaaa tcttctttac aaaaatataa
     1321 tggagaatta ttatttgaaa atagtatcga taaatttata ctaaatatat atataccatt
     1381 ggaccaagat actgacagtt ggggcgatta agttaccagt tgggacatat gtattgatac
     1441 aaaaaaataa ggatgttatc ttttaataac aaaagataac atccttattt ctgtattaag
     1501 gagcttataa ttttactaat agtaagttat aagaattgga taggaaggtg atatgtattg
     1561 aataataaat ctttaatata aagaaaatat aaatatattc tagtgcaatg aataatactt
     1621 tagagcggtt agtgtgatgg gaggattgtg tatgaaagta aaaaggaata taaatagagt
     1681 ggcaataatt gtggctttaa atcttagctt agcattgatg gtaggatgta tgtctcctgt
     1741 atctgataag aacttaagta gctcggaaca aattagtaaa gattcattga aagaacaagt
     1801 tgaagataaa aaagatgaat ctatcgagct atttaataat tactcacaaa agattaatga
     1861 tgcgaaaggt agagaagaag taaaaaaaga tttatctcaa agattttcga aagctataat
     1921 agacagtggt tatagtatta ctaactcaaa atcagatggt agctttgtag tatctataaa
     1981 tggtgctgaa aaaccacctg aaaaaaatat tcgaagttta ttctattcta tgtctgaaga
     2041 cgataaagaa ggaaaagtaa caatagatat tctctgtgct aaagagtatc cagaagatga
     2101 taagttatct gagagtgata aatatattaa atttatatac aatttattta aatcattgac
     2161 taatacgaac ttaactgaga aagaaatttt ttctatggtt gaagaagact ttaataaggg
     2221 agtaggtgtt gtagagcttc cttatatgaa ggatatacat gttgaagtta acaaagttaa
     2281 acaatcaact aaggttttaa aactaagtct agtatacaaa tttgatattt caaatcatta
     2341 gtatattaga aataactata acaatataga aaatatatca tagaaaattt ttttagattt
     2401 tataaattta acaaaaaaag tattaatata taggtgttca aggtagatga aaaaaaataa
     2461 gttattttgc ttccatttta tgctaaagga cagtataata taattaaagt atgttataac
     2521 atacaaaaat gtagtatata ttatccaaag ggggatttta aatgagtcaa actaatagaa
     2581 tagaagcatt tagacaagaa tatattaatt ctaaacctat gatatgttgt gaaagggcta
     2641 gaatttttac agaatcacat aaaaaaactg agggagaagc tatctgtata agaagagcta
     2701 aagctttttt ggagacatgt aaagaacttc ctataaaaat ctttgaaaat gaattaatag
     2761 taggtacagc aggaaaattt agaagaacag ggatattgac accagagttt tcttggcaat
     2821 gggtagacaa agaaatggat acttttgata aaagaactca agacccatat gtaatatcaa
     2881 aagaacaaat agaatttata agaaaagaaa tatttccata ttggaaggga aaatctttgg
     2941 aagaagtttt cttagcaaga atcccagaag atacagctaa gatattagta gatacaggaa
     3001 taatagataa tgattctaag tggagacagg cagttggtga agtaacacca gattatcaag
     3061 atatattgtt tgtaaaagga tataaaggga ttaaggaaga tgcagataaa aaaattaaag
     3121 aattagatat atcagtttca gaaaatattg aaaagataga tttttacaaa tctgtttcta
     3181 tagttgcaca gggtattatg acattggcac aaagatattc aaatcttgca aaagagatga
     3241 gtaagcaaga aacagatgaa aagagaaagt tagaacttat aaaaatttca gagatatgta
     3301 tgaatgtacc agcaaaccct ccaactaatt tttatgaagc aatacaattt gtatggtttg
     3361 ttcaattagg tggtattcta tcagaaaatc cattggcatt aaatttaggt agatttgacc
     3421 aatatatgta tccatattat gaaaatgatg ctagagaagg taaaataact gaatcagaag
     3481 ctcaagaatt gatagaagca ctttggatta agttatcaga atgggtatgg actatatcag
     3541 caaatacagc aaattacttt gcaggatata atcaattcca aaatcttact gttggtggta
     3601 aaaagagaaa tggtactgat ggaacgaatg atatttcata tatgtgttta aaggctacag
     3661 aaagtgtaaa aactcaccag ccaggattaa gtgttagagt gagtcaagga gcaccagata
     3721 atttcgtaat ggcagttgct aaattggtaa aacaaggaac tggtttccct gctatacaca
     3781 gcgatagtgc aggggcacaa atgttattac aagatggata tgatgcagaa gatgctagag
     3841 attggagtaa ctgtggttgt gtagttcctc attttagaaa aacaggacag tggacttcag
     3901 ccgtaaatat caattttgca gcagctttag aatatgctat gaatgaaggt aagagtagat
     3961 taactggaga aaaaatgggg ttagatacaa aaaatatcac agaatttact tcttttgaag
     4021 aacttaagga tgagttttta aagcaattag catatcttgt aaaaagttct gttataggaa
     4081 ctactgttgc tcaacaaatt cataaagaaa tggtaccaag accattttta tctacttgtg
     4141 tagatggatg tttggataag ggagttgatt taagtaaagg aggagcaaaa tataatatag
     4201 gtcctgtatt aactggtata gggttaggtg tagtttcaaa ttcattggca gctataaaaa
     4261 aattagtatt tgaggataag gtgactacgc tagaagaatt aacaaaagca ctaaataatg
     4321 attgggaagg ttatgaagaa ttaagaaaac ttgcgttgga tgttcctaag tatggaaatg
     4381 ataatgatta tgtagattca ttagcaattg aagtttctga tttttattat actgaaacta
     4441 gaaaatacaa ggatattttt ggctctaaat ttaatagtgc atttatgggt atatcaaact
     4501 atgtaccaac tggaaaaata gtaggtgcaa ctccttgtgg aagaaaagcg acaaaacctt
     4561 tgacagaggg agtttctcca tttgttggta ctgatacaac aagtccacta gcagcaatga
     4621 aatctgcatc aaaaataaat catgatgttc atactggtgg aacacttcta aatttaagac
     4681 tgaatcaaga tttggtggaa actgaaagag gacttagaaa tctaacatct atgattaaat
     4741 catattttgc tttaggagga ttccatgtac aatttaatac gatatcaaat gatactctat
     4801 taaaagctca agaaaatcca gaagaatata aagatttatt agtgagggtt gctggatata
     4861 gtactcaatt tgttaattta tcaagagaaa tgcaagatgc tataatagct agaaattcac
     4921 atagcaattt ttaattatga ggtagattat gagtaaaaaa ggtagagttg taaaagtaca
     4981 acatttttca gtaaatgatg gagatggaat aaggactact atatttttag aaggatgtaa
     5041 attaaaatgt aagtggtgtt ctaatccaga ttcctggagt aacatagtaa aattaggtgt
     5101 tatgaaagat aaatgtgtat cctgtaatag atgtatagat gtttgtcctc aaaatataag
     5161 ttcactattt gatagaacgc aaataaataa taaatgtgat ttatgtggag agtgtatcaa
     5221 ggtatgtctt aaagatgcca tttgtataat gaccgaagaa atgagtgttg aagaaatagt
     5281 agaggaagtt gaaaaagatt ttatactttt ctttgaatct aatggaggga taactttttc
     5341 tggaggtgaa cctacacttc agattgattt tttaagagaa ttagttgata ttttttatga
     5401 taaaggcata aatattgcaa ttgaaacttg tggatatttt gattggaata aggtaaatga
     5461 tgtatttgag aaaatagacc acatatttgt agatataaaa tctatggatg ataatattca
     5521 taaagaatat acaggagtaa gtaataaaat tatattagat aatatatgta ggctttcaaa
     5581 gttgaataaa tctatggtta taagagtgcc aattatttat ggagtaaatg atagtgaaga
     5641 aaatataaga aatactgcat tatttgtcaa acaaaatgtt ccaggaggca agatggagtt
     5701 acttccatat cataaatttg gaatcgataa atataaggca ttagggcttg aagattatat
     5761 atatgaattt gatgaaattt gtaacaatca tatgttaaaa ttaaaagaga tagtagaact
     5821 tacaggagta aaaatcatag aatacaaata gctatcagga ggattttaac atggataata
     5881 aatttcctaa tgtgggagaa aatttaaggt tattgagaca agaaatgggc ataagtttag
     5941 acaaagcttc aaagatgaca ggtgtcagta aagcaatgct tggacagatt gagagaggtg
     6001 aatcaagtcc gacagtatct actttatgga aaatttcatc tggatttaaa ataaatttta
     6061 ctacactatt aaatgaaaat acaaatacct atgaggttat aaagaaagaa gaagtagagc
     6121 ctattgttga gcaaaaaggg aacatgaagt tatatccaat ataccctttt agccctcaaa
     6181 gaaggtttga actttttata attgagcttg aggaaaactg tactcatgtt tcatctacac
     6241 atagtcatgt tttagaagag tatgtactcg taatagaagg taaacttgac cttaatgtgg
     6301 atgggaaaac ttatatttta gagtctggac attctattag gtttgatgga actttagagc
     6361 atatatataa aaatttaaat aaaggaaaaa ctatatttca caatatagag gtttatagat
     6421 aattttatag ttatcctttt ctaaaataaa tgaaaatttt agaaaaggat aacttatttt
     6481 tataatgtcg aaaagattta tatgtatgtg atgatgttat tgttttaaaa aaaacataaa
     6541 agcattataa taatattaac aaaatagaat aaagaggaag agaatatgat tataaaggtt
     6601 tcaatgacag tattagtatt aatattattg tatattctgt ataagtatga aactcagcat
     6661 atagaaacga cagaatatac tattttcaac aaaaaaattc caaaggaatt tgataacttt
     6721 aaaatagttc aagtgagtga tttacataat aaggtgtttg ataaaaataa taaggtattg
     6781 atagataaaa ttgaatcatt aaacccagat gttatattta taacaggaga tttagtagat
     6841 ggagaaaata aaaattttca agttgcatta gatttaatag ataatttggt tgaaaaatat
     6901 gaagtatatc atataattgg aaatcatgaa caaaaatctt taataaaaaa gtataaacat
     6961 ctatataagg attattttaa ggaactatat tcaaagaaaa taataaatat ggaaaatgaa
     7021 tctgtaagaa tatatagagg taatagccat cttaatttat atggtttaat tataccattg
     7081 gaatgctatc catatttatt tgctaataac aaaaaattag aactaagtga caattttgta
     7141 gaagacaatc taggtaaagt taatgaaaat gaatataaca ttcttttagc acatacacca
     7201 tttttctttg aaaaatatgc aaaatgggga gcagatttgg ttttagcagg acatgttcat
     7261 ggaggtataa taagaatacc ttttgttgga ggtgtattat ctccaaatag agagttcttt
     7321 cctaagtatg actggggaaa atatgaaaaa gataatagca ctatgatttt aaataaaggc
     7381 ttaggaggct caaaagtttt aattaggctt aattgtaaac cagagatagt taagattaca
     7441 ttgaagtatg agaaataaga ttttatataa catatataaa catcaataac atataaataa
     7501 ggttgatgtt aaatccattt aaacatatat aatgaccaat tttgaataat tgttgaatta
     7561 ataaaaataa agtgctattt ttagcataga atatttaaac tatggggggt agtaaagaat
     7621 catgggtgat acaagtagat tttatgagtc tgtaaaaaat aaatatccga aattaaaaga
     7681 tggaactagg gttcatctgt ggccaaaaga aaacattata attggtccta tttatgatga
     7741 agaagatgga tataaaaaaa gtatcggaaa tgtggatagc ttaagttgga agcaaattgt
     7801 tatactggct gaatgtgatg ggaaaaatac tactgacgat ataataaata tattatccaa
     7861 tagatataga aatatccata atatgaaaga aaaagttatg gaatttttta tgttttatga
     7921 gaatgtatac ttaacgtttg aagatgatat acagacacta tcaagtgtat ttgagataac
     7981 aggaaataaa tcatacatta ctcctttgta ctttactata gaaatagatg gagataataa
     8041 tacaaatgat tatttttctg aaatcacaag tttattgaac tgcatgtatg agaaaggctg
     8101 tagatttata gaggttattg gtgaggatat tttaaaaaat aaaagtatga gagaaatatt
     8161 tcaatatatg ttagaccatt ttgatttaat tgtggtaact aaagatagtt ttactattga
     8221 tagaagctta ataaaggaat tagataatta tcgtcataaa gtgatatgga aagtatattc
     8281 aaaaaatgat gatacacgta ttaaattaaa agaagatata aaaataacca gtttaataaa
     8341 aaggggtcac actgtagtaa gaggagacag agaaaaagca ataaaaatag aagatatgag
     8401 gtttaaagat acaaaggaga ggttttcaaa atatggagca gagtggagtc atttatatat
     8461 ttcttctgat ggtagtgtaa aatcttattc atttcaaaaa gataaaagaa tctatttagg
     8521 caatatattt aacaattctg ttgaagaaat tttcttagaa atgcaaaata aaattgacaa
     8581 actagaaatt gtgtatcaat agggggaaag tttaagattt atagaagatt tatagattgt
     8641 ttatttaagt ttgtgtcaat aattataatg aggtgagatt acttgttaaa attattggac
     8701 aaattttttt atg
//
LOCUS       DAELJO010000158         8713 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELJO010000158 DAELJO010000000
VERSION     DAELJO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.159"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            136..2313
                     /locus_tag="KPB88_002981"
     CDS             136..2313
                     /locus_tag="KPB88_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0055243.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2327..2749
                     /locus_tag="KPB88_002982"
     CDS             2327..2749
                     /locus_tag="KPB88_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055244.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3157..3960
                     /locus_tag="KPB88_002983"
     CDS             3157..3960
                     /locus_tag="KPB88_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0055245.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3953..4621
                     /locus_tag="KPB88_002984"
     CDS             3953..4621
                     /locus_tag="KPB88_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0055246.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4857..5519
                     /locus_tag="KPB88_002985"
     CDS             4857..5519
                     /locus_tag="KPB88_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0055247.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAIGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5579..6376
                     /locus_tag="KPB88_002986"
     CDS             5579..6376
                     /locus_tag="KPB88_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055248.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6494..7684
                     /locus_tag="KPB88_002987"
     CDS             6494..7684
                     /locus_tag="KPB88_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0055249.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7793..8542
                     /locus_tag="KPB88_002988"
     CDS             7793..8542
                     /locus_tag="KPB88_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0055250.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3468 a          866 c         1595 g         2784 t
ORIGIN      
        1 ttgaattatc ttataaacta tgaatgaaaa ataattatat aaatagatta ttaataaata
       61 taaaatctta ttattaaaaa tattcttaga aattacacta tataaaatat aaagtatgat
      121 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
      181 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      241 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      301 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      361 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      421 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      481 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      541 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      601 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      661 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      721 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      781 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      841 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      901 ttagctcttg gaatggcgaa tctatttata caaaagaatt taatagatag agaatacata
      961 gaaaagtatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
     1021 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt cgagatgttg
     1081 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1141 tttcaaaact atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1201 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1261 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1321 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1381 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1441 atgtttccta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1501 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1561 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1621 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1681 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg
     1741 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1801 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1861 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1921 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1981 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     2041 atattaaaaa atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     2101 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2161 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2221 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2281 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2341 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2401 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2461 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2521 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2581 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2641 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2701 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2761 agaatgactt atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata
     2821 atgaaaactc aatatagctt ttttatggat atagttggat actataatgt gattcttagt
     2881 aatagttttt tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt
     2941 cgaagtataa tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa
     3001 ggtataaaat cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc
     3061 agagctatta aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa
     3121 aaaattaaaa taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt
     3181 atgtttgcct tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat
     3241 aaagaagaac aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt
     3301 aaagataaag ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga
     3361 actaattgta aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa
     3421 gctgataaag cagatgttgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa
     3481 tatgcttttt cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat
     3541 actagtataa aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca
     3601 gtagcaaatg aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat
     3661 tcaagtgcga ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta
     3721 gttgcgcagg atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg
     3781 atagaacctc caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt
     3841 gaaaaatgga caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg
     3901 actaaaatat ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa
     3961 ttttaatttt gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac
     4021 aatactaata tctattgttg catttttttt atcaatatta ttaggtgtaa tacttgcctt
     4081 tttcacacag acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt
     4141 tagaggaaca ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt
     4201 ttcttttatg aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc
     4261 atctgcatat atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca
     4321 ggaagcatgt tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc
     4381 tcaagcagct acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg
     4441 ttcttcttta ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc
     4501 tgcttcaagt tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt
     4561 atctataata ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta
     4621 gatttcaata tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta
     4681 attttagaaa aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac
     4741 tgtataatca agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa
     4801 tttaatatgc gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg
     4861 cttttaatat gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa
     4921 ctgtgatttt ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt
     4981 tatcatctta ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt
     5041 ttagaggtac acctcttatg ccacagttat tctttttata ttttggacta gcatatatga
     5101 gtgattttgt taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata
     5161 tgggagctta tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga
     5221 tagaagcagc atatgcaata ggattaacaa atttacaaac aatgaaaagg atagtgatac
     5281 cacaggccgt aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag
     5341 ggtcttctgt agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag
     5401 gagcgttgag ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta
     5461 taataactcc tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag
     5521 ttattaagaa aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat
     5581 gataaaaaga aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac
     5641 tggttgttca aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa
     5701 ttcagctgac ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat
     5761 gtggaaagag attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag
     5821 ttctatattt ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat
     5881 gtctaaagaa agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg
     5941 tatagctgta aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt
     6001 tgtagcagtt tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa
     6061 tttgtttcaa gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg
     6121 aagagttagt gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa
     6181 tatgaaagta agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa
     6241 gaaaaacgat gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat
     6301 gcttgcagat ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc
     6361 tgatatacaa aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag
     6421 tatactaaat ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa
     6481 atcaaaatga gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact
     6541 aaaaaataga gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc
     6601 atttaaagaa tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga
     6661 gtacataaat attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg
     6721 aaaaactata ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt
     6781 agaatttaaa tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg
     6841 gttgttagga acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa
     6901 attactattt caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt
     6961 acttgaaggt gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa
     7021 aattggaatc gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat
     7081 tggcaagggt gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa
     7141 tgcagtatat tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt
     7201 agtagtgtct gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg
     7261 tacacttcaa gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat
     7321 tctagaatat atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa
     7381 gagtacagga ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag
     7441 tgcttgcaaa atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg
     7501 tgaggatttt gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg
     7561 agataaagaa aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa
     7621 atgtcttagt atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact
     7681 atagagtaca ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa
     7741 ttttgatgta gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa
     7801 agtagaaaat ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga
     7861 gataagtcaa agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt
     7921 acttagatgt ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt
     7981 aaaagtagat gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc
     8041 tatggtgttt caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga
     8101 ggcattgata gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt
     8161 aatagataaa gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg
     8221 acagcaacaa aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt
     8281 tgatgagcct acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa
     8341 atctcttgca aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa
     8401 agaagttgct gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc
     8461 tgatgaaata tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag
     8521 gaaagaagaa gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac
     8581 ccccattaaa tttataagtt agataataaa tttaatggga gtatattttt attattagat
     8641 ttattattta atttaaaatt agaaatgtaa ctatttgaaa attcttttta aaagtatatc
     8701 aaaatatcaa ata
//
LOCUS       DAELJO010000159         8371 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELJO010000159 DAELJO010000000
VERSION     DAELJO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.160"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..965)
                     /locus_tag="KPB88_002989"
     CDS             complement(<1..965)
                     /locus_tag="KPB88_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0055251.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDE"
     gene            complement(1013..1873)
                     /locus_tag="KPB88_002990"
     CDS             complement(1013..1873)
                     /locus_tag="KPB88_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0055252.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2142..2351
                     /locus_tag="KPB88_002991"
     CDS             2142..2351
                     /locus_tag="KPB88_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0055253.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2351..2617
                     /locus_tag="KPB88_002992"
     CDS             2351..2617
                     /locus_tag="KPB88_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0055254.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2646..3218
                     /locus_tag="KPB88_002993"
     CDS             2646..3218
                     /locus_tag="KPB88_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0055255.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3453..4643)
                     /locus_tag="KPB88_002994"
     CDS             complement(3453..4643)
                     /locus_tag="KPB88_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0055256.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4903..5334)
                     /gene="dut"
                     /locus_tag="KPB88_002995"
     CDS             complement(4903..5334)
                     /gene="dut"
                     /locus_tag="KPB88_002995"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG0055257.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5447..7675
                     /locus_tag="KPB88_002996"
     CDS             5447..7675
                     /locus_tag="KPB88_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG0055258.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7786..8271)
                     /locus_tag="KPB88_002997"
     CDS             complement(7786..8271)
                     /locus_tag="KPB88_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0055259.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT         2930 a         1319 c         1057 g         3065 t
ORIGIN      
        1 tcttcatcaa acatttttat tatatttttt atctcatact tgtactcatc ttcattcatt
       61 ctataaagca ttaaatcttc tagtacatct ttcatatcga agtacacatc ttctaataaa
      121 taaaaatagc cttcctctat atctttcaat ttatctatag tatccatttt tttacttata
      181 tctttcataa tttctaaatt ttgtatattt acatctatga ttgttaataa gtacattctc
      241 acagttttta ttatttcaac agaaaaatct acaatattat aatttccttc ttttttatat
      301 aatgactcct taaatttgtt aaaacaatta tctaaattag aatttatatc gttaaatggt
      361 ggtcttatta tgtcacgtat tattaaaaga ttttgtatat ctacattatt tgtgattgcc
      421 tcatccaaaa taaataaatt atatgcatca tttaatgggt catgggcatc tccactaaac
      481 tctaagttaa caaattcaag taaatttttc aatgatatat agttcataca tctcatacca
      541 caatttatat atttttcttt tatatctaca aataaatctc taatgttatt tacaaacctt
      601 ggatggttat tttttttagc acttctcata tctgtgttat tgaaacatgt caaatccaaa
      661 ttaccaaaag tatatattcc tttaatatca gaaaatatgc caagccattt tttaaacttt
      721 ctcatgacct cttcataact tggtgcactc ttcaattctt cagaagatat atgtgttaat
      781 tcttgtatat gaggatacaa ctcctcatta cttactggtc ttattaaaga tttaaattta
      841 tcaatatttt ttgttttttc atcatacata actgctccga ttgcaattat atcagaatta
      901 aatactgctc tttttgattt agaatttggc atattcattt caaaatctat atatactctt
      961 ttcaatataa acaccctttc caattgttta tagtgattct ttttaactat ttttattcaa
     1021 aattttctaa gaaagaatgt ttctccccat ctttttgcca accaagtact gaatcaagat
     1081 ttacattttc tgccgcctta aatatagatt tttctacatt tttaaaattt gattctaaac
     1141 tctttattaa gtcttttatc tcatatcttt tattacctgt ttttttagca gcaaccatac
     1201 atgcacaatt taatggccat ataccactat tttgaataaa tcttataata tgttcttctc
     1261 ttatataata aagtggtctt ataattttta taccttcaaa attagtagaa ttaagtttag
     1321 gaagcattgt tttaaaattt ccagcacaca ataagttaag cattgttgtc tctattacat
     1381 catcataatg atgacctaat gcaagtttgt tgcaccctaa ttcttctgcc tttgaatata
     1441 atgctcctct cctcattctt gcacacatat agcatggata atcttttgct atttcatctg
     1501 ctatctcaaa tatccttgaa tcaaataaat gtattggtat atttagatac tcacaattat
     1561 ctataagtaa ttgtcttata tttgcatgat atccagggtc catagcaata aattcaacat
     1621 caaaattaac ttgtccatgt ttttttaact cttgaaacat tttagccata agaatactgt
     1681 cttttccacc agaaatcgct acagctatct tatcaccttc ttcaaccaat ttataatctc
     1741 ttattgcctt cataaactta gaccataaat ttttcctata tttttttatt atactttttt
     1801 ctatatctct tactggaagt ctttcctcaa aaggaactat aatttcacag ccttttcctg
     1861 atagttcact catctaatca cctcgtatat ttatgttaat aatattttac aaaaaagtct
     1921 accttcatcc acaaaatact ataattttta ctttaatttt actaaataat ttatagttta
     1981 caataacaca taaacttata ataacataaa taacctcttt cttttattat cttttttatt
     2041 tttctcattt aatattttat tattagtaac gatttttaaa tgattgaata ttatacacta
     2101 gatagagtta cctatctatt tacaacaagg aggttttcat cttggaaaat aagactagag
     2161 atatcaaaaa aaattgtggt ccatctttgg ctagaccata cattaataat caaattttca
     2221 cagaaatgta ctgcctttct gatgcattaa aatttggaac tatatttcca gaactaaact
     2281 tactagagtc tgaaatgtac aacaaagaat tatatgctaa gccaaaaaaa tatagaggag
     2341 gtagaagata atgaaagact catctagagc tgaacttatg agaaaagttc aggaaacatc
     2401 ttttgcatgt gttgatatga acttatattt agacaatcac cctgatgata aaaatgctat
     2461 caacacttat aattcattat gtaatcaatt tgcgcaagct agatatgctt acgaaaataa
     2521 atacggtcct ttaactaact ttgggtatgc tccaagtaga tgcccttggc aatgggctga
     2581 ccaaccttgg ccttgggaca gagaatttaa ttattagaca tcaagtattg gaggattgaa
     2641 aattcatgtg gatatatcag aaaactatac aacacccagt taatataaaa acttgtgacc
     2701 ctagaatggc taaatttctt ataactcaat ttggtgggcc aaatggagaa cttgctgcat
     2761 ctttaagata tttaagccaa agatatacaa tgcctactgg taatatgcgt gcacttttaa
     2821 cagatattgg tacagaagaa ctagctcacg ttgagcttat atgtactatg gtttatcagt
     2881 taacttctga tgcaagccca gaagagttaa aagctgcagg tcttggttca aactatgctc
     2941 aaaatggata tggaatttat ccaacagatt caaatggtgt tccatttgat gtaagaccta
     3001 tagcagttat gtcaaatccc gtaaccgatt tacatgagga tatggcagct gaacaaaaag
     3061 cacttgcaac ttattatcaa cttataaacc taacagatga cgttgatgtt atagatgtat
     3121 taaaattctt gggtcaaaga gaaataattc attatcaaag atttggtgaa gccttaatgg
     3181 atgcttacga gttagaagaa tctcaaaaaa tgttctaaaa tttttatttg tataactatt
     3241 attatttgat aatttataat ttactgttgt gttaaaattt ataaaaaata taaatataat
     3301 ttattttcta taaacagaga aaataaataa aaagcaggta tcaatcaaat agtaacatgt
     3361 attatactat acattactat caatattgta cctgctttca tattataagc aataaagaaa
     3421 aatctattta ttaggtttta taataaaaac aattataaaa ctcttcttgc atttacatat
     3481 ctacttgaat agtagctact atttatatta gatattgtta ctttttttga tgaacctgaa
     3541 gatgcatgta tcatatctcc atttcctatg tatattccaa catgacttac agaaccatta
     3601 tttgaacctt gtgtatcaaa gaatactaaa tctccacttc taaggtctcc tctatttaca
     3661 tatgttccat acttactttg gtctctagat actcttggta tactaactcc tacagatttt
     3721 ttcataacgt actgcgtgaa tccagaacag tcaaaactat ttggaccttc tgctccccat
     3781 acatatggtt ttccaagtag tgtctttgca aaacttatta cactatcagc tccttgactt
     3841 ggagaattag tcgaataaga tggaattgaa gtatcaacag aacctttttc agcaaggtat
     3901 tgacttgaca catatccagt ttgattgcca taacttattt ttgtccatcc tccactttca
     3961 gaaatacttc caacctcagt accataactt aatgtagcta ttttagaatg acttgtacct
     4021 ggtcctgttc taacgttcaa tgactttgct gttacaacct ttgtaccctt tacaaggtca
     4081 tttgaactgt tttcagcatt ttcatttgta gagtcattta cactagttcc taaatattta
     4141 cttgaaacat aacctactct accattagaa cttatttttg accatccatt tgactcagat
     4201 atgtatccga cttcagtccc atatcctagt gttgatattt tagaactact tgtacttggt
     4261 cctgttctaa aatttaatcc ttttgctgtt acaacttttg tcgacttaac tgaagtatca
     4321 ctactttcat tgctacttcc aaggctattt attgctacat aatttccatg cacataacct
     4381 gtgtttccat tatatttaac ttttacccaa gaaccttctt ttcctacgta ctcaacttta
     4441 tcacctttgt aaagtttccc cattgattca tggtttgtac ctggtccttt tctaaaattt
     4501 accgcattcc ctgttattgt tctgctttct atactcacac tctctacttc agactcatta
     4561 gcatatgcta aattccccat agtaggcatt attatcgcag ttgccattat actggctgct
     4621 attctctttt gattaaaatt cataactttc cactcatctc ctcttttcta ctatctttat
     4681 ttcacttcaa aatataaaag aaataattga cactatcaaa atatcaatta tttcttttat
     4741 attaatatta ttacaaattt gtaactattt gtctatagta atatttgctt accataaatt
     4801 accatgtata aattttaatc tttaatagcc tgtaatttag taatatggcc atcttttacc
     4861 tttttttaaa atggctatat tttttaactt ttttgtaact ttttaaaatc cagttgagcc
     4921 aaacccacct tttcctcttt cggagtcact taattctttt acttcttcta tgttaatatc
     4981 atagataggt tttactatca tttgagcaat tttcatatgc ttttcaacct taaaatcatt
     5041 tttaccatga tttattaata taattttaag ctctcctctg tatccttcgt caatagttcc
     5101 tggtgtattt aatacagtaa cagaatgctt taaggcaagc ccacttctag gtcttacctg
     5161 cgcttctgtc atagtaggaa gctctataca tatcccagtt ttgataagtt tagtctctcc
     5221 aggaggtatt acaacctctt ctatagaata caaatccata ccagcatcac ctttgtgagc
     5281 aaaattaggt attatagcat catcatttaa tttttttact tttaaattgt acatcttata
     5341 tctcctttca actttctata aatattataa cttaaatttc gacctcttga agttttttat
     5401 ttttttgtgg taatataatt attagtgttt taaaggagga ttttttatga aacttgtact
     5461 tgctgagaaa ccatctgtgg caaaaactat agcatcattt ttaggtgcaa agaccagaca
     5521 agatggttac tttgaaggaa atgattatat aattacttat gcagttggac acttagtatc
     5581 attatatgat atgaaggatt atgataaaga taaatactct ggctcttgga aaatgaacaa
     5641 tttcccattt gttcctgagg ataagtttaa atttaagatt gatagttcta aaactaaaca
     5701 attcaacact gtaaaaaagc ttttgaatag aaaagatgta gaatatgtaa taaatgccac
     5761 tgataatgac cgagaaggag aacttatagc atttttaata tttttactgg caaaaaataa
     5821 aaaaccagta aaaagaatct tggtaaatga atggacacca gaagatatta caagaggtat
     5881 aaaaaaccta aaagatgaag atgaaatgag aaatcttcaa gctgctggat atactaggct
     5941 tattactgac tggttgattg gaataaattt cacttctgtt gctacattga aatacggaaa
     6001 tgggaaacta ttaaacatag gtagggtaat tcttccaaca gttaaattag tctatgatag
     6061 agatatggaa atactaaact ttgttcctaa aacttattat gaaatagaag ggcattttaa
     6121 agctgaagct ggtgaatata aaggtaaata tgttaagggt aaagaaagta aatttgatac
     6181 acttgaagat gcaaataaaa ttatagcttc aattacatct gaaactggta aaatactaga
     6241 taaaaaagtt actatgtcaa aggaatatgc accaaaactt tttagtttga catcacttca
     6301 aggatatata acttctaagt attccaattt tacatctgat aaggttttaa atgtctgtca
     6361 atctctctat gagggtaatg gtaagggtgg atacataact taccctagaa ctgattccat
     6421 tttcttagaa gaaagtttgg cttcaaaagc ttctcaaact ttagataaat taaaggttgg
     6481 tcttgaatat gaaaataaaa ttaaattttc taaaactaaa agagtttttg attcttctaa
     6541 agtagactcc catagtgcta taattccaac ttatataatt ccaaaaagtt tatctaagga
     6601 tgaacaatta gtttatgatg ccataaaaga tagatttgta gcaaacttta tgcctcctgc
     6661 tgaatatgag aatacagaaa ttaagactga agtagataat tgtactttct taacaaaagg
     6721 taaggtgctt aaatcaaaag gataccttga ggtatataat aaggaagaaa aaaacgattt
     6781 acttccactt gtaaataaag atgatgttgt agatgtgtta gaaataaaac cattaactaa
     6841 gcaaactact ccacctaaac cgtatactga ggacacactt ttaaaggcta tgaaaaattg
     6901 tggtaaaaat gtgcctgaag aagatacaac agtgctatct ggatattcga ttggaacatc
     6961 agccacacgt gctgatgtgt taaagaagat aagtcaagtg ggctatgtaa agaaaaaagg
     7021 taaatcttat tctataacag accttgggaa aaatttagtt gaaatttttc ctgttaaaga
     7081 cttatttgat gtagattata ctggaaaact tgaaaaaagt ttaagcgata ttcaaaaagg
     7141 aaagtataca agaaaagaat atcttactaa tatcattagt tttatatatc aaaatgtcaa
     7201 tattataaaa caagatgctt caaaaaatat aaacacagaa gcatatactt tcgattcaaa
     7261 aactaaaaaa tttactaaaa caaaagaaaa aaaacagaaa aaagatgtaa ctaatactga
     7321 aaaagaagtt gctatatcaa aggattctaa taacaaaaaa gaagttaaaa atggtgatga
     7381 aaatcaatct cttggcaaat gccccatttg tcaaggtgat gtactagaat ttgacaaagg
     7441 ctttgcttgt aaaaatcata aagaatgtaa atttgttatt tggaaaaatg ataagtattt
     7501 aagtctttat aaaaagaaag ttaacaaaac aatggtcaaa aatattttaa aaaaaggtga
     7561 aactaaagta aaatctttaa ctgctaaaaa cggtaataaa tttgatgcta cattaaaata
     7621 tgataaaaat ccagataccg gctatttcaa ttggaaaata gagtttgaca actaaatatt
     7681 cataagaaaa gaaatagcag tagtataata atctatatta tattactgct atttcttttc
     7741 ttatgaagtt ttaacttttt taatagtgac ttttacaatc acactctaac ctaatttctt
     7801 tactatttct gataacttta atgtatgttc atctaataaa gttagttcct cttcagaaag
     7861 attagataat ttttttacta gagattcttt gagaatagct tttactgcat caaataattt
     7921 aaatgcttta tctgttaatt caaccctaag aatccttcta tcttttgggt caggaaattt
     7981 attgactaaa ccatgttcca ctaatctgtc aataatcgga gtcatatttg attttaaaat
     8041 tcctaaatta gaagctattt gagaaattga tgatgaattt gatgtcataa gataaagaat
     8101 taccttagca tgtgatggag ccattggaca ttcttcaaaa taagctttca ttatatcaga
     8161 gttattttga aactttttta acaaatcgct ttctttaaat atattatttt caagcacaaa
     8221 taaaaaatct aaaaaagagt ccgctatttt aagtatattg ttgttatcca tatttgtaac
     8281 atcccttcaa tagataatat aataaaacat tgttgtttaa tatagtataa attataaact
     8341 attaataaat ttaaaacgag aaaaagttat t
//
LOCUS       DAELJO010000160         8352 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELJO010000160 DAELJO010000000
VERSION     DAELJO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.161"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(172..333)
                     /locus_tag="KPB88_002998"
     CDS             complement(172..333)
                     /locus_tag="KPB88_002998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055260.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            complement(412..912)
                     /gene="tcdE"
                     /locus_tag="KPB88_002999"
     CDS             complement(412..912)
                     /gene="tcdE"
                     /locus_tag="KPB88_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG0055261.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            complement(1034..8134)
                     /gene="tcdB"
                     /locus_tag="KPB88_003000"
                     /gene_synonym="toxB"
     CDS             complement(1034..8134)
                     /gene="tcdB"
                     /locus_tag="KPB88_003000"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG0055262.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEIFKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
BASE COUNT         2789 a         1420 c          832 g         3311 t
ORIGIN      
        1 cctataactt ttaaactatc aattaaatca tttattaagg tttgatactt attttctata
       61 tcattttgtc cttcatcacg tatttttgtg tttttgaata atttccatca caatttacac
      121 atatttctat gtttaactat tatagtttgt gatatctgta actaatgtag cttaatagat
      181 tttaccaact gaccatgcac catctttgtg aaccaatgtt tgatatacta ccttgtcatc
      241 tatatagtaa tgaacacttg cttcgaaatt atatgtattt aatacttgtg tgtctcttgg
      301 catctgctcc ctcgttttca ttattagttt catgaattat gataactttc agctattctt
      361 atttggataa cactttttat ttgttaatat ctttattata tctaccatta cttacttttc
      421 atccttagca ttcatttcat ctgtcattgc atctagtaaa attgctattt tttcctttaa
      481 tctcttaggt actggtaatc cacataagca catatttttt aatatactta cactttcata
      541 taaaataaat aaaatagaga aaaattcaga tattccaaga tgttttagtc ttaaaaaatt
      601 gatacaatct tgtggtaaca taaataaaaa gttaaacttt gtaagaatgt caactactga
      661 taaaaaaaat atacatgcta tcattgctac ttttctgatt cctccatcta ttccaaaact
      721 agaattaaat ttacgacttt ttattgcact taaacatcct aataatgtat caaatgatat
      781 agttaaaatt actaacttta taaatatatg ctctgataaa aaagatattg tcatattcat
      841 aacgcctcct aggtttatat aaaaaaatat tttgttacca ttagaaatat aaaaaggtga
      901 actactgtgc attcatcata gtccaccttt ttattggtaa atcttcatat tagatagaac
      961 actttatgta gtgtatcatt ttgtttattt ttatatattt aagtataaga ggatatattt
     1021 aacatatttt tatctattca ctaatcacta attgagctgt atcaggatca aaataatact
     1081 cctcaccatc aataataact gaaccagttg ctgcaatata ttcatctgta aaataatatc
     1141 tcttttcatc taaatctaac caaccagtat agtttattga ttctccctca aaattctcat
     1201 ccaaagtatt ttgatgtgca aagtatttaa atccatctgg tgtattaaat actccaatct
     1261 gcatgacacc atcttcacca aaatagaaca tcttatcttc tatatttata taaccaaatt
     1321 gcatttcacc attctcatta aagtaataat tattattttc aaatgatata agacccgttc
     1381 tcattatgcc cttctcatca aaataatatt ttatatcatc aattaaatta atacctttgc
     1441 atgctttttt agtttctgga ttgaaataat atttatcact ttcattttcc atatcatata
     1501 tccatccagt ctcaattgta tatgtttctc caaaataata tacatcttcc ccaactctaa
     1561 ctaaaccact atattcaact gcttgtccgt aaatattatc atttacagta ttagcaggtg
     1621 caaaatattt atatccatct gaagtatcaa atacaccaat ttgaactata ccattatcat
     1681 ctatatagaa ataattgtca tctatgtttt gtactccaga ttctataatt ccagagtcag
     1741 agaagtagaa gactttatca tttatagtga caaatccaac ttgcataatt ccatcatcat
     1801 taaaataata ttgaccatca tttattaatg acaaacctat atatgcttct gctgtatctt
     1861 catcaaaata atactttgaa ccatcctcta aatctttcca tccaactaca gctgtaaatg
     1921 aatcatcaaa atagtaaatt ttattattga aatttaatat accagaatat gagatttctt
     1981 caccttcttc atttcctaaa tcttcattat gatgagcaaa atatttaaat ccatcttctg
     2041 tattaaatac tcctatttgc atttctccgt tttcagcaaa gtagaaatgc ttgccatcta
     2101 tttcagtgta acctactttc ataacaccag aatcatcaaa atagtgttta ttatcattta
     2161 tactaacaaa tcctttttgc ataactccat cagaattgaa atagtattta taatcaccta
     2221 tttgatttag acctttaaaa gctttacctg tttctgggct aaaatagtgc atttcaccat
     2281 ctaattcttt ccattctaca gctcctctat aattatcatc aaaataataa atattttcgt
     2341 caataattaa ttttccagta aaatcaattg cttctccttc taggttttca tcaagtgtat
     2401 tagctggggc aaaatattta aatccatctt ctgtactaaa tacacctgtt tgtaacactc
     2461 cactttggtt gaaataataa tttttgtcat caattattgt ctctccaatt gaagcagctc
     2521 caccattaat tggattaaag tagtatttat catcgcctac agtcacaaat ccagttatca
     2581 aattatttac tggtggttta aaataatata atgaatcatt aatatatatt aatccagata
     2641 ccatcattcc aaagttatta atataaaatt tctcattata taaagaaact agacccaaat
     2701 catagccaat caatccatcc tcataatatg aaggtgtaaa tgataagatt atttcactta
     2761 caggtacatc ttgtttatca ctaaagttaa aagatagctt atttgccaaa gtcttatctt
     2821 taaaaacatt aacgaatctt attttaactt gtgacacctt attttcttca ctagttgaca
     2881 taagaataaa atcattacca tcattactcc atacatatcg tatagataga tcattgatat
     2941 taacatttat tttttcattt atataatttg catctaatac aataacttct ggataagtat
     3001 tatttgtttc atatacaggc gttatattta tttcatctgt ataaatattt ggtgaaatta
     3061 caactttatt aacacaactg tctattccat aaagatactt ttgagagaaa ttgataacag
     3121 ttgaagatat atctccagaa tcatctaaat cataatttgg ttccactatc atattttgtc
     3181 tatttcctac atataaagta taattagttt ctagtgtatt aaacttaatg aaatatggtt
     3241 gtatattatc attttcatca caaataaact caaattggcc aatagaagta gtaccactta
     3301 ttataaaatt agcatctaat ataaacttaa tattagattg taagaaatta acgaaaatac
     3361 tttttatatt catactttct aaaaagctca ttaaagaatc tgaagtattt gtattacctt
     3421 ttctattcat gaacttcaaa atctcagcta ctccactttc atctaaatgc acactattta
     3481 actttatagt tttttcatct tgtagagtca aagaaagaga gatttttata tcatccttaa
     3541 gataataacc tgttaatata ttaacattat ctttagttat aactttgaca tctttcaaat
     3601 tattactata atatccaaat gaaggcttac tatcatccat ataaacctta cttataagaa
     3661 ctacatcagg taattcagat acaaataaac cttcttttgt tgaaccatta ataaaaccat
     3721 tctcttttcc ttcactatct acaaagctat atggtatatt tttctgtaat tcgctattga
     3781 atcctatata atctattttc tgttgaatat gatttgaatt taacatcaat atttttaatt
     3841 cgccagaaat aagtaattta tatgatttag ataataaatc aacttctata attgcattta
     3901 ttccttctaa aattgaaaat gttaaagaaa caaatccatt acttccattt acctcaccag
     3961 aaaaattaat ctcatggcta tttaagataa ttttattctc ttcaatactt agtgtagata
     4021 aaataccttc tattaaatca ccttttttaa ttttatcaga ttctatagtt acatctctca
     4081 caacattatc aacatctata atccaaacat cactttcact taattctata tttataccca
     4141 tattatattg agaaagagac aatgcataag ttcctcctga accatagaaa gaatatgata
     4201 atttttctct tatatattct gtagttatta ttggaactat aaaacttcta gtattactat
     4261 ctaaatttat tcttatatta gtatcttcat atcttggttt taatgttgtt attaaagcat
     4321 cagctataaa agcaaaatat ctccaataaa actcaccttc atagttatct cttatacggt
     4381 ctaacagttt tgtgccatca ttttctaagc ttcttaaacc tggtgtccat cctgtttccc
     4441 aagcaaatac tctatttgga gcattaggta ataccattaa atcttttgac aaatcaagtt
     4501 cttctttttg tacttccaat acgtcatata tagataagtg tggctctcta tatgttattg
     4561 atggtgctga aaagaagtga tctatatcat cagttacagt atgacctgaa ccaccttcca
     4621 ttctccagat ttcacattta cctaaaacta ttgaattatt attaaaatct atttctgata
     4681 tcactaaatc atcttgtggc atcattattt tatcatctaa taaagtaaat actccttcag
     4741 tttcaactaa tgaaacatgt ttaaaataat ctacaacctt tgttgcctta tctcgaagta
     4801 caagttcatt gtttactaag cttggtatac ctgctgaaat tcctgctaaa ggaactaaaa
     4861 gtatactaaa tccactagct atccccaaag atgaagtaat gattgcagtt gtagctgttg
     4921 ttaaatttac tgccattata cctatcttag cttctatttc ttgtcttaat aatgggtcac
     4981 tcgtttcact tagctctttg attgctgcac ctaaacttac accatctata atagttgcaa
     5041 ttataggtaa tccttcagat aatgtaggaa gtaagtctat agtttcatct aatgcagttg
     5101 atactaattc aacaactttg gctgcatctg taatagtatt taaaccagta ctaaataatt
     5161 gagcgtaaac ttggactttc attgctacac ttaaattact aagagattct ttagaactat
     5221 tatattctat taatgattgt ataaaaaatg cagcatttaa agtatttact tcgtgtgtag
     5281 tatctaaatt tacttttttt actaacttac catttacagt atcaaatata gtacctttta
     5341 tcttagaaat ctcttcagtt atatgattag catattcaga gaatattgtt ttttcagttt
     5401 ctacaaatat agattctcca gtttctttat taataaatct tatactaaat ccctcatcag
     5461 tctctgatat gtcctcaaaa gatataaaat gagaatcttc taattcattc tgttgtttta
     5521 agtcacatag tgcatcagaa atagattcta ttagtttaaa ttcatctttt atataattaa
     5581 tagagtcaga agttaaattc ttagcttctt caatcctttc ctcaacaatt tgcgtatcta
     5641 tatttgaaat aacatttatc tcacattctg ttaacattac tttttcttct agttcaatat
     5701 cacttgaatt agaattattt ctaatttctt gtaataatgt agatagctca ggtaaatttt
     5761 tagattttac tgtaatttta ttttctttag gattaaatga tatatattct tttgatgaaa
     5821 tatcctttat aatactttct tctttattta tccattcacc agaatgatcc aataattctc
     5881 ttcttccttc actatttatt ctaacttcat attgatttgc acttactata atagagtctt
     5941 gacttataga tggcattaat tctgatattt tatctttaac tttaagtaat aattttccag
     6001 gataagtctc ctctacgttg atagagtagc taaacatatt acatcctaat aaatttattt
     6061 ctattgactt aggagaaata tcctctttag ctaaatctat tgctgcttct atttctgtgg
     6121 ataatgaatc tacatcaaaa cctgcaaata tatcagtatt aaattcatct ttaccatgac
     6181 caataaatgt taatttaatc ttaggtctat cagaaattat acttggaatt ttatacttgt
     6241 ctatttcttg tatttcacca tctccaggat tataataata tgcaatttct gaatcttcta
     6301 tatttttctg aaacagtaca ctatcataag gagtctttgc aaataagtta catgctgctt
     6361 cataactaat tttatctcct tgtaactgaa caatatagtg tatatatcct ctctctgaac
     6421 ttcttgctaa tgaagatatt ttttctaaaa gatactcctt gtcaactact atattttgag
     6481 aaaaatcaag attatcatct tcaccaagag aaccttcaaa ataattcctt ttatattctt
     6541 caaattgagc tttagctctt gcatcgtcaa atgaccataa gctagccatt tcttgttcag
     6601 ttgattgaga aatattagtt ttaaagattt caaagtttct taaatcagct tctatcaaat
     6661 ggatattcat actgccttct ttaaacatta ataaatcttg ataagctgcc gcatatgctt
     6721 cagggccact taagttaata gtagttttaa catctgggaa gaaaccaact cttaaatact
     6781 ttcctagttc catcataaat ctaccattat ctgcattagc ttcagccatt atactatcaa
     6841 taaaggtatt cgttgtagta ttaaaatcat tatcctcgct aatagctgga tttaaactat
     6901 tattcaatat tttatatcta ttctcgattt gttttactat taaattgcta caatatgagt
     6961 ctttcacaga aattagccct tgatttataa tacccttact attaaatgca attttaactt
     7021 ctagtggtga tgcctccata tcaccaagtg atgagaatat ttctgattta tctgacttag
     7081 aagctagaac agattcaaaa ctactttgaa cttcttcgtc taacatgtca aaatgttctg
     7141 aggtatattc tggtatatat tctttgtatt tcattatagc ttctaacttt gtcatttccc
     7201 aaaaatccac tgttactgaa ctaggtttct ctatagactc aaataagtct ggttgtattc
     7261 ctggtaacat atcaacatct aaatacatac caccaatttc ttttaatgca gatattctta
     7321 atatgtcaga agcagcagct aaattccacc tttctaccaa ctcttgttca tataagttga
     7381 atgactctcc atttttaaat tcttcaaagt ttctaacatc atttccacta ttctgtgtaa
     7441 ttttatttaa ggattcttca atataggtat taagttcatc tatctccttt gaatactcat
     7501 ttgaaagata tgtctttaca atatcatcaa ttataagttc aggattttct tctctttgag
     7561 ctttatagta gtttatgaaa tttttctgtt tatcataaat tatttccata cgttttctga
     7621 agaatttatt atagtcaaat ctagggtcat ttaagttttc tctaaatgat tcaagtgtat
     7681 catttattgc tgattctact acagtttttt tcaatgtgtt tatcaaaaat gcattactat
     7741 cataaaaaac attaacatta taatcactat ttacatcttt ccattgattt atataattaa
     7801 tagcagtgtc atttatttga cctccaatcc aaacaaaatg taaatttttc tcaactggag
     7861 ttaaattatt attctttagc tctaatactt ctgtaactag atattcctta aattttttta
     7921 aggctttatt tctaccagat tttttatatg tatctatata aatatctgtt aaactattta
     7981 tatcttttaa ttttaaatat ttttcgacta cagtattctc tgacatatta tgatattctt
     8041 ctaaagcatc caatattgca acatattcat cttcttgagt acgaaatctt acatttgcca
     8101 ttttttctaa ctgttttcta ttaactaaac tcataaaatt ttctccttta ctataatatt
     8161 tttacatcta aatgataaaa ctcttttata tatcctcctt tctatttata aaatatacta
     8221 atatctactt aggttttcat atacattcac ttcctaacat tttaaattga agacactcag
     8281 ttgattaatt tgctcttcga ttgaataaag acgttgtctg aaataaatat gtaaactttg
     8341 ttctatataa aa
//
LOCUS       DAELJO010000161         8343 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELJO010000161 DAELJO010000000
VERSION     DAELJO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.162"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            145..1242
                     /locus_tag="KPB88_003001"
     CDS             145..1242
                     /locus_tag="KPB88_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0055263.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     IFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            1233..1931
                     /locus_tag="KPB88_003002"
     CDS             1233..1931
                     /locus_tag="KPB88_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0055264.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            1934..2998
                     /locus_tag="KPB88_003003"
     CDS             1934..2998
                     /locus_tag="KPB88_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0055265.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     FNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            3181..3885
                     /locus_tag="KPB88_003004"
     CDS             3181..3885
                     /locus_tag="KPB88_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055266.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            3878..5200
                     /locus_tag="KPB88_003005"
     CDS             3878..5200
                     /locus_tag="KPB88_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055267.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            5387..6760
                     /locus_tag="KPB88_003006"
     CDS             5387..6760
                     /locus_tag="KPB88_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBG0055268.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(7132..8070)
                     /gene="pdaA"
                     /locus_tag="KPB88_003007"
     CDS             complement(7132..8070)
                     /gene="pdaA"
                     /locus_tag="KPB88_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0055269.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         3076 a          920 c         1377 g         2970 t
ORIGIN      
        1 atttttagat ggtctctttg tattttttaa ttaaaacttt aagatttctt aagaattata
       61 ctaggttttt ctaaagattt aaagtctata cttataagta tagtaatcat atgaaaagaa
      121 aagctttaac gaggagggaa tcttatgaag gtattaattc ttacaggaaa atttggtatg
      181 ggtcattact cagcatcaaa ttctttaagt gaagatataa aagcaaaatt tgataattca
      241 gaaataataa ttaaagatat atttgagtac attatgccta actattcaga taaaatgtac
      301 aagacttttt ctattcttgt aaatcgggga agtagtcttt ataatttatt ttataaatgt
      361 gctgaaaatg gaaagaaaga tataaaattt actttttcag attatttttt aaataaatta
      421 gatacattac ttcatgaagt acaaccaaca gtagttattt caacttttcc attttgttct
      481 caattagtat caagatataa ggaaaaatat aactcaaatc ttcctttaat aacatgtata
      541 actgatataa gtagtcactc agaatggatt agtaaaaata cagactgtta tttagtagct
      601 agtaaatcta ctaaggaaga acttattttt aaaggaatag atgaatctaa gattaaagtt
      661 aatggtattc cagtaaaaaa agaatttaaa agaatagaac atgtaaatca ttcaactaaa
      721 aaaaatattc ttataatggg tggaggatta ggattactgc ctaaatcaga acaattttac
      781 aaagaattaa atagtttaga aggagtaaaa actactgtaa taactggtaa taataaaaaa
      841 atgtactata aactatatgg tagatatgaa aatatagagg tagtaggtta caccaatgaa
      901 gtatataaat atatgaaaga ttctgacctt attatttcta aaccaggtgg aataacacta
      961 tttgaaacaa tttattcaga acttccaata ttggcattta atccattttt acaacaagag
     1021 atagataatg ctagttttat attaaataat gaaattggga gaatacttgg taaaaataaa
     1081 aaatactatg tagatgagat aaaagattta atttatgatg atgctactct taaagaaatg
     1141 agcagtaaca tgaaagaatt aaaaaaacaa tttgataata ataccttaga gaatatttta
     1201 ttttcacttg atgaacaagg agcgtgtaga gaatgtatgt agtaggttta attatcgttg
     1261 ttgcattgat ttttttagtc cattcaatta tacctacata ctataataaa ctattaaata
     1321 aagaggtatt aaaaaatatg gctggagaaa atgaaattgc acttacattt gatgatggtc
     1381 cagataagag atatacagaa aaattactag atgtgttaaa agaaaatgat atacaagcta
     1441 tgttttttgt agtagcaaag aatgctgaaa aagagcctga aattataaaa agaatgttga
     1501 gagaaaatca tatagttggt ttacattcat tggaacacag aaatgcatgg ctttatagtt
     1561 atagttatgt taaaaaagat tttattgaaa gtacaaatat aatgaaaaat ttaggtgtag
     1621 atgtaaacta ctatcgtcca ccatggggtc atactaatat attttcaaat tcatttgtta
     1681 aaaaatataa tctaaaaatg actttgtggg atgtaatggc agaagattgg gagaaagata
     1741 gtacagtaga cattattata aacaagttaa tgagtagaac aaaagaaaat tctataattt
     1801 gtttacatga tgcaggtgaa aattcaggtg gtgcagtagg tgcacctgag cgtactattg
     1861 aagctttaaa gatagctatt ccaaaactta aagccagtgg tttaaagttc gtaacaccag
     1921 aaaggatgta gttatgataa gtaaaactga aaagaaaaat aaatgcctag gtagtgtagc
     1981 ttttttagtt ttactaatgg gcataacagg atattttgtt tttagagggc agtcagttga
     2041 atcattgata aaatcattaa aaggtgcaag ccctatgttt atactaattg gatttgcaat
     2101 gatgtttata tatgttgcct gtgaaggtat aaatatatac ttaggtatga aagctttaaa
     2161 ccaaaagact actcttttaa aatgtatggg atatgctttt attggatttt attttagttc
     2221 tattacacct tcagcatcag gtggtcaacc agcacaagtt tattatatga aaaaagacga
     2281 tataaatata tcatattctt cacttatact attagtcatt gttgttattc accaagtagt
     2341 tatactggca tatagtggaa taatgttcat tatggaacgt gagtttatat taaataatgt
     2401 aagtggtatg aacattttac taatatatgg tgtaataaca aatgttgctc ttgtcattgg
     2461 agtaattgca attatatttt caaaaaaact tgtaaacaat tttataatat caataacaaa
     2521 tttattaggt aagttaagaa taataaaaga tgttgaaagt tcaagaaaag ttataatatc
     2581 tcaaattgaa gagtatgtta aaggtgctca atatataaag cagaatccaa aacttgtagt
     2641 ccaaatttta gtcataacta ttgtacaaat aacagctatg tttttagttc ctttctttgt
     2701 atacaaagct tttcatctat caacatatac tgtatttgag atattagcga tacaatcctt
     2761 gtttaatatt gctgtttctt cattaccatt gccaggggct gttggagctt ctgaaaatag
     2821 ttttatgaca ttatttaaga tatttttccc tggtcactta ttagttccgg caatgttact
     2881 tagtagggga ataagttttt atgcatttgt agctataagt ggattgatat gtatagtagt
     2941 acatgtaaaa tcttctgaaa aagtaaaagc agtgaaaaaa gttgcttatg taagataata
     3001 ttatatagat tatttttatt aatatgtcaa tttgtgtgta tattttaatt aagctttaaa
     3061 catgtttaca ggagtagaaa ctattaaaac gtgtgacatt gctaagcttt tgctaataaa
     3121 gtataatatt ttatatagtt tgaaatattt aagaaatata gcaaaaaaag gagcttggtg
     3181 atgaataaag agattccaaa aaaaatttta gtagctgatg ataatcctga gattagggaa
     3241 atcgtagaaa tacttttaac tggtgaaggt tatgaagtta ttatggcaac gaatggagaa
     3301 gaagctgtaa acttagtaga tagtacgatt gatttgatta ttttggatgt tgtaatgcct
     3361 gtaaaaacag gatttgttgc atgtaatgaa attagagaaa aaacgacagc accaatatta
     3421 tttttaactg caaaaactca agactctgat aaagttattg gattttcagc aggaggagat
     3481 gactatttat caaagccttt ttcatattct gaactaattt caagggttaa gtctctactt
     3541 agacgttatt atatatacca gggaaaggaa aaggaaagag agccaaatat attaaaaaca
     3601 aaagagttat gtgtcaattt agatactcaa gatgttacac taaatggaga acctgtgttt
     3661 ttaacaacta tagaatatag tatattaatt cttatgttga aaaatagaaa aaaagttttt
     3721 tcgtctgaaa atttatatga aactatttgg gatgagcagt atttttatac agcaaataat
     3781 acaataatgg ttcatattag aaatttaaga aaaaagttag aaaaagacca aaaaaatcca
     3841 caatatataa aaaccgcatg gggaaagggg tattatattg attaacaaaa ttaagccatc
     3901 taagttggga gttatactat tgatatatgt agtaatctca gctgcagtgg ctttcatttt
     3961 aaatatacca ttttactctg ctgttgaatc tatagtaaaa gatgtatcag aagggaaagt
     4021 atattttaaa agagaagtaa atcaacttat ggataatttt caaacttatg tgaataacaa
     4081 tggtataaga gcagatgatg gagatgatat agcaaaatgg aatagtgata attggtttgt
     4141 ttctatgaag atatataaag gaaataaggt tttttatgat acattatact atcctaatga
     4201 agtacctgat aagagtgagg aaaatgagtt ttatagcagt cccatggcta gaaactatga
     4261 gatacaattt tctgatatta aagctaatat agttgtagta gctttattta gatttagatt
     4321 tcaaaatttt atagaagcaa tttctattac atttatggca cttacattta ttataacatt
     4381 tttactgttg tttagtagaa agataaagta cctaaggaaa atagaaatgg gtataaagat
     4441 agtagagagt ggcagtttaa agtatagaat accagtaaaa ggtaatgatg aattatcatc
     4501 acttgcatct agtattaatg agatgagtca tacattggag aatcaactta taaaagaaag
     4561 ggagatgaag aataaaaata aagatatagt aacttcaata tcacatgatg ttagaactcc
     4621 attaacgtca gtaatttgtt atttagattt gattaaagac cataaataca atagtcctga
     4681 gcaattggaa caatacataa aaaatgtaag gtctaaagca tatcaaataa aagatttaac
     4741 agatgacctt tttacgcatt ttgttaattc tggagaggaa gtttcaaaag ggtatgaaaa
     4801 aataaatgct aatgaattta tttttcaatt acttttagat agttcatata cattggagga
     4861 gaaaggattt catataacaa tagaaaatga actttataac caatatcata tttatgtaga
     4921 tgttacacaa tttagaagga tatttgataa tttatgttct aatataataa aatatgctag
     4981 gaaagataag cctatagtat ttttaattaa agtagaggat tctaaattac taattataca
     5041 aaaaaataga gtaagtaatt tttctaagaa tgtagaaagt tttggaattg gtataaaaaa
     5101 ttgtgagaaa atagttgcat atcataatgg aagcataaat gtaaattcag atgataaaga
     5161 atttgctatt gaaatatcta ttccagcaaa agaatcataa aaatctaaat tatatattgt
     5221 gtaattgcta ttatatattg tgtaattgct tatgtaaaat tctatgaagc aggaagcatg
     5281 tgactttagt tatgggatgt tcaatattgt gtacctctag gcaagtaaca agtttactta
     5341 taagaccata taatattatt atcttaaaaa aaggggacat aataatatgg ttttgacagg
     5401 agcacattat atttacattc tatttatggt aattatatta attactatga ttatgaaaaa
     5461 ggatacaata gtaccatgta tattaggtgt tttttttatg gggttgttct ttgaaaaaaa
     5521 catttttggt gctataacag cagtgtttaa ctcttttatt atatctttaa atgagttggg
     5581 tccaattatc ttgattatag ctattatggt tgcattatct aaagccctag aagccaataa
     5641 tgctatacaa tatatggtga gaccattttc gagagttata aaaaattcta atacagcatt
     5701 ttttgtaact ggatttgtta tgttagttct ttcttggttt ttctggccaa ctccagctgt
     5761 tgccttagtt ggagctgtat ttttacctgt tgctatgaga gctgggctac cagcaattgg
     5821 tgttgcagta gcattaaatt tatttggtca tggtcttgcc ttatctacag actttgttat
     5881 tcaaggagct ccaagtataa cagctggtgc agcaggtgtt gcagtatcat atgttataaa
     5941 tgatggtatg attttgtttt gggtgatggg aatagtaacc atatctatgg cattttatac
     6001 attaaaaaga gatattaaca aaggaatgtt tagagaagaa ctaaaaggtt ttgaaagtga
     6061 agaagtagaa gagtttaatg gaaaatctaa aatagcaact attttagtag ctttgggatt
     6121 tcttgctgat ataattgcta tgtatgtatt tgacctaaaa ggaggagatg catcagctct
     6181 tttgggtgga acagcagtat ttttaataat aattataaat gttataaatt ttggcaaaga
     6241 tagtcttgaa aatgtatgtg agaatataat tgatggtttt gtgtttggga taaaaatatt
     6301 tggagcaatt ataccaatag cagcattttt ctatatgggt gaagttgcac ccttgacagg
     6361 tgtatttgga aaagtattag caccaggttc acaaggttta ttatcagata taggcatagc
     6421 tctttctcaa acaataccac ttaataaatt tgccgtatca ggtatagaaa ctgtagtggg
     6481 agcaataact gggcttgatg gttctggatt ttctgggatt tcattagttg gttcacttgc
     6541 ctctgtattt ggaactgcta taaatgcaag tgttggtgca ttagctgcat taggacaaat
     6601 cagtggtata tgggtaggtg gaggctgtct agttccttgg ggtcttattt cagcagcggc
     6661 aatttgtgga gttagtccaa tagaattggc aaagcgaaac tttgtcccag ttataaccgg
     6721 attggttgta acaaccatag tagcaatgtt tattatttaa taaatcgaat aaatctttaa
     6781 ttgtataaat taaaaataga tgtgtagcta taatttatta taattgtttt actaattcta
     6841 agccatacat ctatttttat tgatattttt attagtattc tttaataaaa taatattttt
     6901 cattatacta tacataacat ctaaatataa catctaaaat actgttatct atatttaata
     6961 tcttgccaaa ttttttgatg ttttattgcc ctgtcccacc aacatatata agctatcaca
     7021 agtttttacc tatactatca ccaaacaaaa attctaattt taatttatta ttttataacc
     7081 tatattatat taactatatt agtttgtaga ctttagatgt aattataaat attaatactc
     7141 caagagttca aattcatatc ctagttttct tgcatttgat ataaaatcat ttaaaatttc
     7201 tgtactagtt ttggaaactg catgtagtaa caagatagaa ccatcatgta gattatccat
     7261 tattttttgc gttgcttctt catgtgatgg ttgtttatca gtatcccagt ctctgtatgc
     7321 aaaactccaa aatacagtct tgtatcctaa atttttagtc attgccaagc ttttttctga
     7381 gtattttccc ataggaggtc taaaaaattt aaccatatct ttcccagtta catctttata
     7441 taacttttca acatcatata attcgtcatt aaagtttttt agattgcttg tttttgttgg
     7501 catagatgga tgggttttac tatgatttcc aactatatgt ccctcagaaa ccattctttt
     7561 tactaaatct ttattttctt ttatatatgg agctgtaaca aaaaaaactg ctttaacctg
     7621 gttttgtttt agagtgtcga gtatttttgt agtgtatcca ttttcataac cttcatcaaa
     7681 tgtaagatat agtgtttttt gaccatcttt tgtaggtcca ttgtataggg catcatagtc
     7741 agaaaattta aattcaatgt ccgtatttac ttctggtgtt ttatgcttat tgtttggtat
     7801 ataaaaccaa tcaagagtag tgttatctaa agaagataca tctaatttat tattatcatt
     7861 attagtagtg gcttttgatg gtttatctgt tccgtctgaa actattacat tggaagtatc
     7921 tttaccacta tctatttttt ccttatcttc ttgtaactgt gtttccttat tttgattttc
     7981 gttttgattg ttttgtgaat ttgaacatcc aacaataaaa aaaatagaaa tagaaagtag
     8041 tgttaaataa tgcaatactt ttctaaacat tttgaagctc ctttctaaat taataataat
     8101 tgcatataat aataattata tgattaatta caattgcata tagtattatt ataattggaa
     8161 attactatta ataatagtta tatctttaaa aataaagtca caatagtatg acaaagggtg
     8221 aataatcaat aaatttaaaa aaaaatgaaa aaaatagttg accatattat gagaagatgt
     8281 tattataata aatgtgctca acaagaagta acaaaataac aagcacaaag aagttaattg
     8341 aat
//
LOCUS       DAELJO010000162         8147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELJO010000162 DAELJO010000000
VERSION     DAELJO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.163"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(60..407)
                     /locus_tag="KPB88_003008"
     CDS             complement(60..407)
                     /locus_tag="KPB88_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055270.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(401..802)
                     /locus_tag="KPB88_003009"
     CDS             complement(401..802)
                     /locus_tag="KPB88_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055271.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(812..1045)
                     /locus_tag="KPB88_003010"
     CDS             complement(812..1045)
                     /locus_tag="KPB88_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055272.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(1045..1944)
                     /locus_tag="KPB88_003011"
     CDS             complement(1045..1944)
                     /locus_tag="KPB88_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG0055273.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(1956..2519)
                     /locus_tag="KPB88_003012"
     CDS             complement(1956..2519)
                     /locus_tag="KPB88_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0055274.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(2564..2794)
                     /locus_tag="KPB88_003013"
     CDS             complement(2564..2794)
                     /locus_tag="KPB88_003013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055275.1"
                     /translation="MKEYMIWFKSGNSISGIVDEDVADKLMQDFMEADSDCKDLKVYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(2837..3034)
                     /locus_tag="KPB88_003014"
     CDS             complement(2837..3034)
                     /locus_tag="KPB88_003014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055276.1"
                     /translation="MFYLLLQVISICLSITVFMYVTGNIVFKIYLKSLRKEYESNIHE
                     VSDEILKRIRKELNKSLDESL"
     gene            complement(3035..3427)
                     /locus_tag="KPB88_003015"
     CDS             complement(3035..3427)
                     /locus_tag="KPB88_003015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055277.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     VNRKGEVKKTICIKYKNGVIRATNGDWIIKDSEGKIYSCTCDEFEKYYEDLNNETKIS
                     NGGLECLREIALKDVNFKFELTGIKEEK"
     gene            complement(3759..3920)
                     /locus_tag="KPB88_003016"
     CDS             complement(3759..3920)
                     /locus_tag="KPB88_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055278.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
     gene            complement(3923..5284)
                     /locus_tag="KPB88_003017"
     CDS             complement(3923..5284)
                     /locus_tag="KPB88_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0055279.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIEKDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVYITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKVLV
                     NEFSKKFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCHCRTTTAPWFEDEEGYRAARDENGKTYYVPSGMKYK
                     EWYEKTFITDKKDDILSNKSWLKADFPTEKKFKKHVEKHLNEYGNITAEEYLSTARNL
                     LSEQLSNDVEGFLSKDNFLFKYRKSTNDFAVGRADGKISTLFKPKEGYNYWLEQIKEY
                     KEV"
     gene            complement(5274..6704)
                     /locus_tag="KPB88_003018"
     CDS             complement(5274..6704)
                     /locus_tag="KPB88_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0055280.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGNKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(6719..7972)
                     /locus_tag="KPB88_003019"
     CDS             complement(6719..7972)
                     /locus_tag="KPB88_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0055281.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSIGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(7950..>8147)
                     /locus_tag="KPB88_003020"
     CDS             complement(7950..>8147)
                     /locus_tag="KPB88_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055282.1"
                     /translation="SRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLS
                     KDDIGDDELKISVDYGDRNDS"
BASE COUNT         2394 a         1539 c          825 g         3389 t
ORIGIN      
        1 ctccatcttt ttacaaactc ttttaaactc tctaaaatca acactgcccc atctagccat
       61 tatgctttat ccttaaataa ctcaagtatt atttcttgat gatttggata tatagctgat
      121 tctccacttc ttacatactc tttatcattt ataataagtt ttgaacctgc tttaatttct
      181 atatctggag atataaagag tttaatagtt tgctctagct tagctacttt cccttctgta
      241 gcagaaacta tatttttata tgaaagttta catggttgat tttctaatac aatcacttct
      301 ttattgttag ttcgttttgt tacagggtct ttaattggct gatactcaac tatagtacat
      361 ttatctctat ataacatttc tattgctttt ctagttttac ttaccatctt aagcacctaa
      421 aggttaatat cttattctta ccataagtag taagataagc tactaagcta tcaaagcgtt
      481 gttctggtgt ttgagagcca cttcctatag caaaatctac ctttgtatca ccttctgata
      541 tagacttttc tacagcttca aagttaatgc tttctatatc taattgcccc atatttttct
      601 tggtaaataa gaactctcca actatcatat caacttcaat ttctttcaat tcaattggca
      661 tagtttttat attacaatct agtttaataa tattttctat tttttctctt acaaaatcta
      721 ttaaccactt atccccatct tttaatatat atccaaaact ttcaagtctt ttttctatat
      781 catcaattat attatttccc ataattttca cctacttttt agtaagttta tttttctctt
      841 taagctgttt attttcttct tctaaagact caacttttga ccttaaaata ttattttcag
      901 ctattaaatc ttttacattt aatgacttgc cataccttac taccttacca gtttcatcta
      961 tcaaatcata tcccatctct aagaaatcat ctattttaca ttcttctata gttaatattc
     1021 tatttaattt ccttacttgt gccattatgc tccagctcct tcaacaacaa attgtattgc
     1081 atcagctttt ttatttaata taaatacatc ctcaaaactt tcttcaaagt agaagtattt
     1141 tccctctgta actgctgttg gttcgtctaa cttagagaac tgataagaaa caggtgtaat
     1201 tattgcactt gggtgaacta aggacataaa gatttgttta gctcctgctc ctactttcca
     1261 tccagttgta aaatcatatg cagttttcat tagattagat ggtactttaa ttattttaac
     1321 tgtgtcaata tcagttgttt gacgattaag agaagttcct gcatccttta tatttactgt
     1381 tctttgtatc tcttttgcat ttttgataag tgtatttact actggagtaa catacaatat
     1441 tcttccattt tcaggtactc tagcttctgt cattttttcc attaacttat caaatacttc
     1501 taatacgttt gttgttgtaa gaacagttgt atctgctgta ttacctaatg cggtccaatc
     1561 agcatatatt ttagatatac agtaagcatc catctctgga aacttttgtt cctcattata
     1621 tacttttgtt atattgccta ttgaagccac ataattagtt tggtttatat ctgctggatg
     1681 aaccaatgtt gaccatttcc tttgattagt taatacctta ggttcccaag cattatcata
     1741 gtttctttga gctactgcta ttgtatctct gtttgaatct actcttccag ttgtagatat
     1801 agttggtatt tctattgttt tagaaccagt ccatctatat cttccattat ttggtgttgc
     1861 atacaaatcc ccgaagttta aagtataagg atatgcttgt gctaaaacat ttgaatattc
     1921 ttttgcataa tttagtgctg ccattttatt tcctcctatt tattattatt ttcatgaggt
     1981 cttaccccag taaaattaaa accaaaatca tttatcttag gctcttgccc tggtgttata
     2041 gtatctattt taggttcttc accttctagt gttgcattaa acaaataatc tttatcctgt
     2101 ttcaaaggat ttatttgctc ttcaaaagct ttttgtctgt ctttactatt tcttagtgct
     2161 tctatatcta aatgagcttt taatgctatt tcatctctac atttaacaga tttaaaagca
     2221 tcacctaacc agtaattaaa atctttttct tcaatttctt ttttgtaggt ttcttccaaa
     2281 gttttcttat cagtttcata agttgttttt agattctcta catcttcttt tgtcatacct
     2341 ccttcaaact ttttaatagt ttcattagct gtattaagct gtgtttcaag atttgtataa
     2401 tcttcttgag taattgtagt ctcttttatt ttcttttcta tagatttttg aagagaagct
     2461 acatcaattt tgttatcctc tacttttatt ccttctagca attcttttaa ccaatccatt
     2521 ttaaatatct cctttcattt tttacaaaat aaaaaagccc tatctaggac ttactaaaac
     2581 caatattatt attctcatta caattattta ttgatatagc ttctatttgt gataaatcta
     2641 ttactgttgt tccatcttca tctaaatata ctttcaagtc cttacaatct gagtcagctt
     2701 ccataaaatc ttgcattaac ttatcagcaa catcttcgtc tactattcca gatatgctat
     2761 tgccgctttt aaaccaaatc atatattctt tcaataacag aaacctcctt ttgttttcta
     2821 cctaataaaa aacttcttaa agactttcat ctaatgattt atttaattct tttcttattc
     2881 tctttaaaat ttcatcagaa acttcgtgta tattagactc atattctttt cttaaacttt
     2941 taagatagat tttaaagact atattacctg taacatacat aaatactgta atacttaaac
     3001 atatacttat tacttgtaac aacagataaa acatttattt ttcctcctta attcctgtga
     3061 gttcaaattt aaaattcaca tcttttaatg ctatctctct taaacattcc aatcctccat
     3121 tagaaatctt tgtttcatta ttcaaatcct cataatactt ttcaaattca tcacatgtac
     3181 aagaataaat tttaccttca ctatctttta taatccaatc tccattagtt gccctaataa
     3241 ctccattttt atattttata cagatagttt tctttacttc acctttacga ttcacagagt
     3301 ttaaagattc atcaagccat atagttccct tctcaaaagc ttgataaaac catataggag
     3361 tattaggact tcctaatatc catttaaaag cttctacttc ctctgatttc tttttaaatt
     3421 tagccatatt atttatcccc ctttaaattt ttaatcatat cttcattgct aactagtaaa
     3481 aaaaatagta tcagtatcac acctaaaata aaattaagca gtggaaataa cgctgggaaa
     3541 ataaaattat atttttttct tcttctgata ttcttacttt taagtaattc aattaattcc
     3601 tcattactat caaccttcat gttgaataaa taaagccctg tacaaaacac aattattgat
     3661 aaaataagta tagatataat ttaagcattt caaatcaccc tcgcaataaa tttttacata
     3721 ataaaagcac ctactaattt ataacttagc aagtgctttt atattacttt tttacctttt
     3781 tcataagcct ctttagcttc ttttaatgac attttatttg gtcctttgta attagtttct
     3841 tttggtccac tgttttgcca attacaatta tcacaaatat caaatacatc tacatcttta
     3901 ccacatactg gacaattcat gtctaaacct ccttatattc ttttatttgt tctagccagt
     3961 aattataacc ttcttttggc ttaaataagg ttgatatttt accatcagct cttccaacag
     4021 caaaatcatt agtactcttt ctatatttaa ataaaaagtt atctttactt aaaaatcctt
     4081 ctacatcatt acttaattgc tctgataaca aatttcttgc agtagataaa tattcttcag
     4141 cagttatatt tccatattca tttaagtgtt tttctacatg ctttttaaat ttcttttcag
     4201 ttgggaaatc tgcttttaac caactcttat tacttagtat atcatctttt ttatcagtta
     4261 taaatgtctt ttcataccat tctttatatt tcataccaga tggcacataa tatgttttcc
     4321 cattttcatc tcttgctgct ctataacctt cttcatcctc gaaccaagga gctgttgttg
     4381 tcctgcaatg acaatgaaat ggtggagctg ttattccaac ttgataatct ttcatatcaa
     4441 atacttttcc atctaactct ctgcatatat ttgatgtttt taaatctagt gtggcaataa
     4501 tctcatactt ttctacatct aaatcattaa aacaatcttt tcttgaagct gatgcaaaga
     4561 aagctgattc agtcattatt aaattcttag cttgtgattt agatacatta aacttcttag
     4621 aaaattcatt taccaaaact tttggatttt cacctctaat aattgattga gttagcttag
     4681 tatgtaactc attgattaaa gcaggtctat gcttacccca aattctttca ctaaagttta
     4741 atccatcact agtccaaggc ttagaaataa ctttatttat tctgttagta tcaagactca
     4801 ttaaactcca accaacgttt actccttgtt gaacattaaa agctgtatga tagtatccac
     4861 ttgtataaat atctctcatt agtttatcaa taccatcaag ttcatttcca tataaaactt
     4921 ctacttgttg ctgtatttgc agttttaaag cttcaagcct tgttatataa actcttgcac
     4981 tagcattttc taactctttc atccactttt gatttatagc attttcttta ccatatttaa
     5041 tatattcttc tacactccat ttaaactctt ctagttctct tgtatttagc agtttcttag
     5101 cttccaataa agatattccc tcattttcag tgaatctgtt ataccatata agtatatctt
     5161 tttctatatt agacattgct agtttatatt gtttttctaa ttcaagataa tattttatac
     5221 ttttattatt ctgagcttct tctaattgtt caaatctctt cctccaataa tctttatgtt
     5281 tcatctataa caccatcttg attattagga attaaatcat catactcttt ttgagtatct
     5341 tcctgttttt taagtctctc aagttcgtca ttaacatcct cgacccaagg atggttagaa
     5401 acaatagttt catctgatac aattccagtt gatttagctg ccatatctat cttttcagct
     5461 tcatttatta tcatagagtg attaaaagta atttgaactg ttttataatc atagctctta
     5521 ttaccactta tctttaaata ctcacacaca aaccataaaa gctctctaat tgctttttta
     5581 aacttctttt cagtcttaga acattttaag tcaagtagtg aatataaaaa tttaagtgct
     5641 acacccgatt tgtcacctgt gttttgagat tctggattaa ctccttgacc aaagataatt
     5701 atattctttt ccaatctatc aagaagctcc tttttagctt caactggtat atttatctct
     5761 agtttatcaa ctccacctcc accatctact ttaattgatt tatagtatct tatattatct
     5821 ataaactctt gtagacttgt tcctggatat tcttttaata cataaataac ctcttgtatt
     5881 tcatctaagt tatctgctag tgtagaaata ttattgtcat atatatctat taatgattta
     5941 tagaaagtta aatctgagac acacttttca ttatttttaa aaggtataaa tggaacttta
     6001 ccccatccct gttctttgtt atttattcta aaatgacctt cttgtatatc agtcattttt
     6061 ccatattcat catataaaaa ttcttgaata aaactattac ctctttcaat aaagtaagtt
     6121 acgtcatttt ctgtgtagta ctcaactctt tttattttat ttccatctat atcttcaata
     6181 taataaaacc taataaatgc aactaattcc ctctgtcttt tactatccca aataggaatt
     6241 gcttcttcag ctggaattat tacatattta aactcacctt ttctattaat atatggatgt
     6301 aaccattcaa cccctttatt actagcattg agatatagtt ctgttattgt atcgtcaaac
     6361 tcttctccta gtaagtcatt taaaagctta gtgagattat catcatctgc attaaatact
     6421 atgggatttc cgacactata gcctaccttt tggtcaacta aaagcttatg gtagttgtta
     6481 attgctttat tattaacttt agtaaaatca tcaaccttag ctccatctaa gagataatat
     6541 cttctcttat tgtttatatc tgcattgcca taatagtatt cttcaccttg tttatatttc
     6601 tctggtctat gttttaaaat ataatgctct ataactttta ctaggttaaa tgtgctctct
     6661 ttttttaact gaacttttat taaatctgtt tcacttatat aaatatttaa cgcctccttt
     6721 actttaagaa gcttattcca ttatttttaa gcttattatc tatagaatat ctaagcgcag
     6781 ccattgcatc atccataaac tcaactggtt catcaagata taatccagtt ctttcatctt
     6841 gtttccattt ccattgttgt atttctttta tggtattagt gcaactagga tgtacatgta
     6901 ttcttaattg tttcaaataa tctatttgag ctttaacact tcctggtcct tttttaactc
     6961 ctttagcttt atatcctgca ctcttccaca tcttaattct atctggttca gcactatcac
     7021 agtacataaa tagagtcttt tctaaaccta tactatttgc aatctttatg atttctgagg
     7081 tatccatttc atgtacatat atttcgttac atatatataa ctctccatcc ttaaagccaa
     7141 ttctaagtac tacatttgca tggttaaatc caaagtcttg tgataacctc atattgtcaa
     7201 aatactcaaa ttctgtagga aattcatgta taacataatt tttaagtatt gctccaccag
     7261 tttctcccca ttctccaaga ccatagactt tgtacccttc tgggtcttgc tcttttctca
     7321 tttgcattct tctgtagtaa gcttcatcta tgaatctatt ttgtagataa gtactatgat
     7381 gagtaaatat atcatcattt ttatagtcaa aatactttct ttttatccaa tgagtagctg
     7441 agactggatt aaatgtaaat gtcatttgat agtataggtt aggattagtt aaaatacctc
     7501 ttaaacggtc atctagtatg tctatgtcac tttccataag ttctgtagct tcttcacacc
     7561 aaacccatgt taattttcct ttcgagaagt taattgattt taatttttct ctttgttttg
     7621 catcattaac tcctctgaaa attatagagt taccagtaac tttactctta atttctaaag
     7681 gatttaaagt agttttccaa tacttatcag cttgtttacc ataaatacga tttatagctc
     7741 ctgtaagctc tgcatacgtt gaatacttat gtgtagcttc tgactttcta actactaata
     7801 gattagctcc ttgatacttc ttatctccta actttagtat ataatcctgt gccacattaa
     7861 cagattttcc actccctgct gaacctttca ttgctctgta tcttttttta gtaaaattag
     7921 cttccttgaa atctggatta aaatttactc taactatcat ttctatcacc ataatctaca
     7981 cttattttca actcatcatc tccaatatca tccttactta ggttatcaac ttcacatttc
     8041 aacttctcaa ctcttgtttt ctgctcctct gtagccaaat tccaatcctt atgaatcatc
     8101 tcatcatatt gtttaattaa acttctaagt tcactcatag ctctact
//
LOCUS       DAELJO010000163         8140 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELJO010000163 DAELJO010000000
VERSION     DAELJO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.165"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            130..2001
                     /locus_tag="KPB88_003021"
     CDS             130..2001
                     /locus_tag="KPB88_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG0055283.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(2093..3028)
                     /locus_tag="KPB88_003022"
     CDS             complement(2093..3028)
                     /locus_tag="KPB88_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG0055284.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            3548..4060
                     /locus_tag="KPB88_003023"
     CDS             3548..4060
                     /locus_tag="KPB88_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG0055285.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(4215..5276)
                     /locus_tag="KPB88_003024"
     CDS             complement(4215..5276)
                     /locus_tag="KPB88_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG0055286.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(5400..6293)
                     /locus_tag="KPB88_003025"
     CDS             complement(5400..6293)
                     /locus_tag="KPB88_003025"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG0055287.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(6303..7784)
                     /locus_tag="KPB88_003026"
     CDS             complement(6303..7784)
                     /locus_tag="KPB88_003026"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG0055288.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(7892..8123)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2953 a         1102 c         1151 g         2934 t
ORIGIN      
        1 aaatttaaaa tttaatgtta caatatagtg acaaaaggta aaaattcaat attatagcat
       61 taattgtaga ttataatgta taataatgtt tgagagaata tattttataa aatgggtagg
      121 tagcgaataa tgaaaaatac aaaattttct ataaaaaaaa taaaaaatat atctaatgac
      181 ccgaaagtcg tacatactaa gggtgccctc cttgaaaaga tgggtaagat ggaaagcgca
      241 atagagttgt ataagagtgc tgcattggat aattacgtaa aatctcaata tactttagga
      301 aatatatatg aaagtaaaaa acaatttaaa gaagcagaaa agtggtattc aatggcatat
      361 aaaagtggaa gtgaagatgc tgcttttgat ttaggtaata tgtactataa gttagatggc
      421 tatgaatatg ctatatattg gtatgaaaag atagcaaatt taggttattt acaagcacaa
      481 aataatttag gtgtatgcca ttttaaaatg aaagattttg taaggtgtga aaaatggtta
      541 aaaacagcag cagacaagaa tttaggtaaa gcttgtttta atttaggggt attatatact
      601 tttttggaga aagatgatga agcatatgaa tactataaaa aaggttctgt gcttttagat
      661 gatgatgcaa aatataattt agcaatatta aatggaaaaa agaaagataa taaatcaaca
      721 gtaagtttat ataaacagtt gtacaagtca ggtcatatga aaggttgctt taacttgggt
      781 atgattatgg aaataaatga taatttagaa gatgcagaga agtattataa aaagagtgct
      841 gatagagatg atgtgaagtc tgagtatagg cttgcatatg tatatgatag aaaagaagag
      901 ctgggagatg caatttatta ttatgagaaa gcaatagaaa aaaatcatac actttctaag
      961 tatagacttg ctaatttata taatagagaa aatgagatag agaaggcaaa aacttattat
     1021 aaaatggctg ctgaagatga tataacagag gcaaaaaata atctggcagg tatatatttt
     1081 gaagaaaaag attatgaaaa tgctattaaa tattatgagg atgctatagc tgttggatgc
     1141 aaaagttccc ttgaaaatct aggggattta tattatcaga atcaagatat agaaaaagcg
     1201 atttcgtatt attcgaggat tccaaataat gtatcttgtc aaatcaaatt aggaaatatc
     1261 tatgaagatt taaataatat tgaagaagcc ataagttggt ataagaaagc atctgaaaat
     1321 ggagatacga ggtctagtta tagattgggg tgtatatatg agagcttagg aaatacaaaa
     1381 aatgctagaa agtattttga aatggcaagt agcaaaaacc atatgaatgc tcgtatacat
     1441 ctaggtagaa tttattttag agaaggtaaa ttggaagagt ctaaaatgat gtttgataca
     1501 cctgccaatg aaaataatgt gtatgctcaa catatggtag gattaattta tgatatgttc
     1561 tataaagatt atgtaaattc taaattttgg tatgaaaagg ctagagcaca aggttgtgtt
     1621 gaatcaatat ataatttagg tcaaatttat ttaaaattaa atgatgacgc agaagctgag
     1681 aaatattata aagaaggtat taaatatgga agtaagaagt gtgagtatat gcttgcaggt
     1741 ctttattata aaaagagcct agatatgtac gtttctttag caaatgaaga gtacgataat
     1801 tgtgaagaga tagtagaagg ctttcctaat ttaaatatag actttgatga agtattaatt
     1861 cctccattta agttgcaaga agaggtaata gatgaagaag agtatgtacc aatttatata
     1921 ctaaatataa aagaaagttt agattcaatg ctagaagggc ttgaaactga aatgattata
     1981 gatgaagaac atgatagtta aaaagtatta taagattata tagatttaaa tatataaaag
     2041 ccattagaat aaatgtaaat caattaattc taatggcttt tttattaata ttttataata
     2101 gcttatttct tgacatgaaa aatgtaatca ctatacatat tatagaggta ataagaagta
     2161 ttatccaaaa tccgtttggg ttattagaaa atggcatgtt gatgacattc ataccaaaga
     2221 atcctgatat tattgtaggt attgagaaaa ttagtgtaac tactgttaaa atttgcataa
     2281 cattattctg attattactt ataattgcac tgaaagcatc cataattcta ctcaatatat
     2341 ctccatatat tttagccata tcaagagcct gtctattttc aactataaca tcttctaata
     2401 ggtcttcatc atctggatac ttctttatac cagcagttct aaccattttg tttaaaacaa
     2461 gttcattaga ttttagtgag gtagagaagt ataccaaaga tttttctaat tccaataatt
     2521 gaaccaattc tttgtttttc attgatttgt aaatttctcg ctctattatg attgtcattt
     2581 tatttatttt tcttaagtaa tgcaagtaat aagctgcatt tttgtgaaga atttgaagca
     2641 aaaagcgagt ctttttaaac gtaaaaaaat ctttaatatg accaactata aagtcattta
     2701 aaatcttagt ttgtgcatca caaactgtta caatagcttc ttcagttagt ataataccta
     2761 agggtattgt agtataatga gaagaattag agtcactttc gtctactggt atatctataa
     2821 gtataagagt atgattgtct tcaacatcta ttcttgagcg ttcttcttca tctagtgcag
     2881 attctatgct ttctacatct atatttaata aattagaaat ttcgttgatt tcactatgat
     2941 taggttcaac aagatttatc caacagccat cttcaaaaaa actaagtttc tctaattttg
     3001 aatctatagt tttgtaatat cttatcatgg caacacatcc tttattcagt tttatataaa
     3061 tctcatcatt taatagtatc tattaattaa ctaatttata taactgaata tatgtattag
     3121 ttttgatttt agatattatt aagtgatgaa aatgtaaaaa attctctccg caacggacta
     3181 gtatccattg cccatcactc tccttattta ttattttata tatagctatt tatgaaatgc
     3241 gtattttaag cactctttaa tataatacct tttccaggta tataaatcaa gttttgattt
     3301 gattaaataa tatttttacc ataatttaca cataaagtat ataagttata tttaatttga
     3361 gtaaagaata tatatattct gagtgaaatt attaaatata ttaaaaaata agagtgtata
     3421 tatatattta cttatttgca ataatatatt taaaaaagta ttttattttc aaaactagac
     3481 ttcatttata atttatcatg aaaataaaag gaatataaat ataagagaat gagaggttat
     3541 tagagatatg agtgatttta aagaaatttt ggcagaagag attaaaaata atccatttgg
     3601 attgataggt aaagattgga cacttataac agcagaaaat gaaggtaaag tgaatacaat
     3661 gactgctagc tggggagggc ttggagttat gtggggaaaa gatgttgctt ttgtagtaat
     3721 aagaccacag cgttatacta aggaatttat agataataca gataaatttt ctcttacatt
     3781 ttttgatgaa gattttcgta aagagctttc atattgtgga aaagtatctg gtagagaaga
     3841 ggataagata tcacagattg gatttaatat agaacactta aatgatacac cttattttaa
     3901 agaatctaaa atggctatta tatgcaaaaa aatgtatagt cagaaacttg aacctcaatg
     3961 ttttattgct gaaggtatag atggaagatg gtatccacaa aaagattatc atactttata
     4021 tatagctgaa ataaccaatg tacttgtaaa agaagattag ttataaaggt taatactatc
     4081 tctaattaaa ttaaaatttt cataaaagga aattgacaca aaaaagctaa ggacataaat
     4141 atgtttataa agattaataa ataacgtatt taaactcctt agcttgataa aattttaatt
     4201 tagttttctt taaattataa ttcagaaatc aattcattga taacatcttc aacagtactt
     4261 aatttatcaa ttttataggc atcatttcca caaaataaaa gtgaattttc aacatctcct
     4321 ttaactgcat tgattagagc ttgagaaata cagtaaggag tttcttttgg attacaagga
     4381 atcaaacaat tatagcactt ctttatttct ggacgattta ttttaacttc ttctataaat
     4441 ttgtttctta tggcgcgtcc aggaagacct acaggacttt taactatatg aacatcttct
     4501 tcactagcat ttatgtatgc cattttaaag ttctcatggg catcacattc gtaagtagca
     4561 acaaatcttg taccaacttg tacaccactt gcacccatat ctatatattt tttaacatca
     4621 ctactagaag ttataccacc agcagcaact actggtatgt ttctaccaaa tttttctcca
     4681 taagtatttg caacctttaa aatttcaaca aattccttat catagtctat agaatctatg
     4741 ttatcaagtt cctcgttaga gtatccaagg tgaccaccag cttttggacc ttcaacaact
     4801 attaagtcag cagtagtttt ctcttttcta tcccacattt ttaaaattac attagcagct
     4861 tttgcagttg aaactatggg tgctattttt acattacttc cctttactaa tgatggtagc
     4921 ttattaggta atccagctcc agatattata aggtctacac cagctttaac agcttccttt
     4981 acgtatgttt catattcttt catagctacc ataaaattaa taccaataat accaccattt
     5041 gagatttctt tggccttaga aatatgtttt tttatagctc ttaaatttgc atttatagta
     5101 ttagtttcaa aatcttcctc atcatatcca atttgaactc cagaaattac accaacacca
     5161 ccaagcttag caactgcacc agctagtgat gacctagata taccaacgcc cattccacct
     5221 tgaattatag gaacttttgc aactaaatct ctaattatta actcttttat gataatccct
     5281 cctcaatgta aacatgttta aagaactaga ttataatact taatattatt acctagtcct
     5341 aattattata ctacaaatta taataaaaga gtagttatat tttgtataga agacgataat
     5401 tatattatat ttaaaacagc tccattagaa tcgcaacata actcttttat ttcccaatca
     5461 gattttagtt tttgtaggta atttttcata ttatccacaa aactatcatc attttctttt
     5521 attaaagaca ttatagtagg tccagctcca cttaaaaata ctccaatact atttaattgt
     5581 tcagattttt caacaatatc attgtagttc tcaattagag ttcctctata atcttgatgc
     5641 aatttgtctt gacaagctac tttaagtagg tcaaagtttt tgtttagtaa ggcagatatt
     5701 agtaaagcaa ctctactaac attgaatacc ccatcagaat atggtatttc ctttggtaaa
     5761 acacctctag ctttttctgt tgataactta aaattaggta tcattgcaca aaatttaagt
     5821 tcttcaggaa tttttataat atcataatgt atgttctcat tatcagtaac agatacaatc
     5881 atatttccta aaatagcagg agcaacatta tctgggtgac cttctatttc tgaagctata
     5941 tttagtaatt gatttttatc taaattagca cctgatagag catttgcagc tataactcca
     6001 gctactatac aagaggcact actgccaaga cctctacata taggtacttc actttggatt
     6061 tttattttga taccagcagg tattttttct ggtttaactc tatcaaaaaa gtacttcata
     6121 gatgtataaa ctaagttatt ttcgtttttg taagcatctt cacaaccttc tatttcaaga
     6181 ccactttcaa tttcttcaac ataaaattta ttgtatatat ttaacgcaat tccaagacaa
     6241 tcaaatcctg gacctatatt agcacttgtt gcaggaacta taatctctaa cataataatc
     6301 acctatacct ttaagaattt ttttatagct agttttaatt catctttttc ataaatatct
     6361 ttatgtagta tttctgcatt tttaagattt ttaataggtt ttggaatatc tattttagaa
     6421 atacttgata atctatcaat tatagcaaag tcatcaaggt ctttaaaatc acaatctata
     6481 gactttagaa catcttcaga aaacttgaat ggacttgcag tacttacaat aacagttttt
     6541 gtgttatcag aagtttcttt tttatatttt tcataacaat tgtatgcaac agcagtatga
     6601 gtatctatca aataattata atttttaaac acattgttta tcgtgctttt aacttcttct
     6661 tcatttgaat attctccata gaatgaggct aaattttttt ccatatcatc acttattttg
     6721 tagactcctt tatcactaag gtctgataaa agcttattta caatatcagt atttctattt
     6781 gatatttcaa acagtaatcg ttctaaatta cttgaaatta atatatccat agaaggagaa
     6841 gtagttaatt tcaatgttct atttttatca tatacaccag ttttaaagaa atcataaaga
     6901 acattattat catttgatgc acatatcaat ttgtttattg gaagacccat ttgtttggca
     6961 taatatccag ctagaatatt accaaaatta ccagttggga caacaaaatt tattttatca
     7021 tttaaattta tctcactatt gctaactaat ttcatatatg aataaaaata gtatgcaact
     7081 tgtggaagaa gtcttcctat attaattgaa tttgcagaag ataatatata attattatcc
     7141 aatagttttt gattaaattc tttatcagaa aaaatcttct ttacacctga ttgtgcatca
     7201 tcaaaatttc cttttatacc aactacatat gtattttttc cagtttgagt tttcatttga
     7261 agtttttgta ctgggcttac accatcttct gggaagaata caattatctt tattttatct
     7321 atatctttaa atccctcaag tgcagctttg cctgtatctc cagatgtagc agtaagaatc
     7381 acaacatctt tctctaaatt atctttcttt atagatgttt taagaagatg aggcattata
     7441 gtaagtgcca tatctttaaa tgctaaagtt ggaccgtgat atagttctaa gaaataagta
     7501 tcatttactt tatttaatgg agctatacta ctacaatcaa acttttcatc atatgcattt
     7561 tctatacaat ttttaatttc atcttcagta aaatcacata aaaatttact aagtacaaaa
     7621 aatgctatct gagaatacgt taaatttact aaatttataa gttcattttt tacattggga
     7681 aattcacttg gaacataaag accaccatca cttgatattc cttttattat tgcttgtgat
     7741 gcagttactt tttcttcggt gcctctcgta cttgaataat acatttttta taatcctcca
     7801 tatattttaa atttttaatt agctaattaa attagataca gaatatttta aaaactgaat
     7861 ttaggtaata aaaaaagacc ctcatcccta aaaaagggac gagaatctgt ttcccgcggt
     7921 tccacccaaa ttgattttaa aaaatccact tgatacttta acgcaagtaa taaaaacgtc
     7981 tattcctact aaaatcatta agataacttc ggatagaagc tcctaggtag ttttcaatcc
     8041 ttctaattag ggaaacattt cagctagtag tttccttctc tggtaatcga ttcagattta
     8101 ctcttcctaa tcaatgcctt tcaatattct taatattatt
//
LOCUS       DAELJO010000164         8133 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELJO010000164 DAELJO010000000
VERSION     DAELJO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.166"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            1..272
                     /locus_tag="KPB88_003027"
                     /pseudo
     CDS             1..272
                     /locus_tag="KPB88_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            580..834
                     /locus_tag="KPB88_003028"
     CDS             580..834
                     /locus_tag="KPB88_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055289.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            870..1193
                     /locus_tag="KPB88_003029"
     CDS             870..1193
                     /locus_tag="KPB88_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055290.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1466..1618
                     /locus_tag="KPB88_003030"
     CDS             1466..1618
                     /locus_tag="KPB88_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055291.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1812..2734
                     /locus_tag="KPB88_003031"
                     /pseudo
     CDS             1812..2734
                     /locus_tag="KPB88_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     repeat_region   3001..3491
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3001..3029
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            3682..3834
                     /locus_tag="KPB88_003032"
     CDS             3682..3834
                     /locus_tag="KPB88_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055292.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            3954..4768
                     /locus_tag="KPB88_003033"
                     /pseudo
     CDS             3954..4768
                     /locus_tag="KPB88_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            complement(5310..5774)
                     /locus_tag="KPB88_003034"
     CDS             complement(5310..5774)
                     /locus_tag="KPB88_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055293.1"
                     /translation="MDFGEVKSELSDKGLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIDIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKSILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(5910..6227)
                     /locus_tag="KPB88_003035"
     CDS             complement(5910..6227)
                     /locus_tag="KPB88_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055294.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6510..6806)
                     /locus_tag="KPB88_003036"
     CDS             complement(6510..6806)
                     /locus_tag="KPB88_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055295.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(6825..7238)
                     /locus_tag="KPB88_003037"
     CDS             complement(6825..7238)
                     /locus_tag="KPB88_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055296.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     VIFNFCNAFSRKDIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7222..7455)
                     /locus_tag="KPB88_003038"
     CDS             complement(7222..7455)
                     /locus_tag="KPB88_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055297.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7674..8048
                     /locus_tag="KPB88_003039"
     CDS             7674..8048
                     /locus_tag="KPB88_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0055298.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
BASE COUNT         3276 a          874 c         1294 g         2689 t
ORIGIN      
        1 tgattaggaa tcgtaaacta tctcgctcaa tttctgatgt gtcatggtca gagtttattc
       61 gtcaattaga atacaaggct aattggcatg gtagacaaat tgtaaaggtg ggtaaattct
      121 ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa cgaggaagtg aaggacttaa
      181 atataagaga atggatttat cctagttgta atgaaactca cgatagagat ataaatgcaa
      241 gtataaatat attaaaagaa ggattaagac taaattgcat tcaaaagcta aagtggaaac
      301 ctagtgaata tatgaattta gaagttaatg agagagcatt agcagccgac tcaatactta
      361 ttaaaattga agatgaagaa gaagcaatga aagaagctga aagagagaaa aagagaggta
      421 gaagaaggta gaatttaaag ttaaaatagt cacattgcta atatatgaaa atgttacaat
      481 tttggaaata gatggatata tatgttgtat agtataatgt agataatata tcaccaagta
      541 aaaaacaaca aataaatcta aattatgagg ggtgtattta tagaaaataa agatattaaa
      601 cttacaatat gtatatttgc ttttgtaatt ggaataatag tgttattttg ctgtataggt
      661 ctaggtcaat ttaatatgga ttgtattata aaacaaaatg gtgggtcaat agctaccgaa
      721 caatacaaca tatatttaga acaatctatt tcacaatata gaaattttgg ttctatactg
      781 gttttactag gaggaatagg aatactgttt gataaaagtg tcagaaaata ctaaaaaagt
      841 atatttttat ttaggatgtg atgaatgata tggcaagaga tgtatttagt gtaaagatga
      901 caccactaga agcacagaaa atggtagaaa aaaatttgga agcagattta gtttctgttg
      961 atatttataa cctagaaaat aataagtcta taatagttac aatctttgaa aaatattatg
     1021 caagaagcaa tagtgatgca ggtttggtag tgatttgtga aaatactaca ggaaaaacac
     1081 ttgttaaaat tacctcaact ggtagtgcaa ttggactatt tcagattgat tggggaacag
     1141 gtaataattt gattaaaaga gtgaaaaaaa tacttattga ttatattatt taattatttt
     1201 atgtagatat tcttaggttg gtatggaatt tgtactaacc tattaagtaa tcaataaaac
     1261 taaataaaca tagaaagcac ttacaaatat gtaggtgctt tttattgaaa aaatagaaaa
     1321 taaaataaaa agtaagatat aggaaaaata tgtaagaact atatgttata ataattgtag
     1381 caagaagatt taatctacaa tttatagagt ggagttcatg tattaaaaaa ttatctccca
     1441 acgataagaa gggaggtgaa tatacatgga taatttttta tttaatgtgt tagctagtgt
     1501 aacagctagt tacatagttt atttgattag caagttaatc aaaaaagtaa aaagccactc
     1561 taccgcaaag agtgacttaa agattgatat aaattttaaa tatcatcgta aaaaataact
     1621 ttcagttata gaacttcact ctaccgcaaa tagattgtag ttcttcttgc ttttattata
     1681 ccacaaatta gaaaaaatat tgcatataat attttttata gtcaataaaa atatgaaatt
     1741 ttttatacac aatgatgatg aatctaacta gatgaataaa tttaattgta aataagaaag
     1801 tacttactta aatggtaggt gcttttgctt tgccaaatta tgctataata ttgagtgaga
     1861 tgagtaattt gtggggaatt ttacaatgtg gatattagta ccaataataa caatagtctt
     1921 acttataata gctgtttctt ctatgcaata tattttagtt atgatagctt ttttgttgat
     1981 tatatattct tttatagaaa agaaaatagt tatgggtctt gtgtcagttc tttttttcac
     2041 ttattcaatt tatctttgtg caacttggga agataaatcc ttgatagcag ataataaagt
     2101 tgagactgtt aaggcacaaa gagaaactgt agaaagagaa aaagaaatgg aaagaagaag
     2161 agtacaagaa gaagtagaca aagaaagata tattgaaaaa catggaatgg aaatatcaga
     2221 aaatgattta aaagttaagc tagaggcttt agtacctcaa gaatataatg ggaagaaata
     2281 tgaattaaaa gtaggaaaat ttaaaagata tagtatgtat tttgatttaa ctgtacaaaa
     2341 tgaaaaattt agtaattctg aagaatgtaa aaaatttgta aaagaaattg ctaatgatct
     2401 taaaaaaatt aaaataagca aggtgtactt taagtttcat tcaaaagatg acggtggtat
     2461 atataattct gtttatattg attattttag aaacatacaa aataatgtag ataatgttga
     2521 aaatttagaa ttcaatgagt ttaaacttaa aaccgaagaa gaagagaagc gagaacaaga
     2581 gaaaatagaa caagaaaaaa acagttataa taactacatt caaaacagag tggttgaccc
     2641 actagataga ataaagaaac taaaagagtt gctagattca ggagcaatta cacaggaaga
     2701 atataataaa aagaaaaaag aattattaga atagataata tagtaagcac ttacaagtat
     2761 gtaggtgctt ttaaatttac aaagtattcc attttaattt tatagtttag attttatgat
     2821 ataataaaaa tatagaagtt ttgcagtgtg cgatatttgt tacaaagtag ggcttaatac
     2881 ttgaaatcta agatgttgag ggtgcgtgat aagtgttatc aattgcacta ttgcccgctc
     2941 actgcaattt taagagtatt gtatatatgt aggtattgga aatgctaagt ttattttggg
     3001 gttttagatt aactatatgg aatgtaaata aagaaggtgg agaacaatat gcatgggtaa
     3061 agtggcgttt tagattaact atatggaatg taaatgtatt aattaaacct aaacaggtta
     3121 caacatctaa cagttttaga ttaactatat ggaatgtaaa tgcgcttaac tttctctttc
     3181 acttctattc ctccatttgt tttagattaa ctatatggaa tgtaaataga atagaatcta
     3241 acatatatac acctccaatt aagggtttta gattaactat atggaatgta aatcactagg
     3301 acaggcactc cacttctaaa gcttatactt gttttagatt aactatatgg aatgtaaatc
     3361 tcgaatcagg atataactat ttaaaagaaa acatagagtt ttagattaac tatatggaat
     3421 gtaaattgct gtgatttcac taataaaaca attaataatt gtgttttaga ttaactatgt
     3481 ggattcaaaa ttaaataaac aaagaaagca cttacaaata tgtaggtgct tttattctgc
     3541 tcaaaattgg tcggttgggt aaaataatta gaaaaagtta gtaaaaacct attgactgta
     3601 actcgttaca atattgttat taatgtaacg agttacagaa aagaggtgaa taaaatagca
     3661 actaaaagta gggcagagta tatgaaaaat cgtcgaaaag ataaaagagg ttttagtgta
     3721 cttttagaca aagaaaagtt agataaattt gatgaagtat tagaggaaaa gaatctaacc
     3781 aagaaagaat ggctagaaga aaaaatcgac gaggaactgg aacaaaagga ataaaaaata
     3841 agggtcactc ccaccgacca aagtttgagt aacccctatg acgtatacta tcgtatatca
     3901 attatagtat atgtcattcc ttaaaaaaat caattattaa ggagtgtaat attatgaaaa
     3961 atgaattaat gatgtttgaa ggaaaagaga ttgaagtatt tgaatttgaa ggaagaattt
     4021 tattcaactc aaaagatgta gtaaactgtt tagacataaa aaatgttaat gagaatataa
     4081 gacttatgaa tgaaaagcaa gttgtaaagt taagaaattc ggatatcagt aatactgaca
     4141 tccgaaaact taataatgct ggagaaaatt ttcttactga aagtggagtg tataaattaa
     4201 tctttaaatc aagaaaagaa gaagctgaga gattccaagg ctggataagt gatgaagttt
     4261 taccaagcat aagacaaact ggtgcataca taactaataa tgcaagcccg aaaagttgag
     4321 agaaaaagca agtgagattg aaaaattaca gttggcttat aacagtacat ctatgttaaa
     4381 agaattgcta gatgatgcag gctttgacaa taaatcaaaa ctattaacag ctaaaacatt
     4441 atataagaaa gcaggaattg atttaccaat agagataaac gaagaagaac attattttga
     4501 tacaaaacaa atagcatcta aactgaaaat atattctaag agtaataaac cagctcagat
     4561 ggcagtttgt gagattatta aaaagattga tttagaagat ggtgaagtta aaggtgtttg
     4621 ggaaactaat ggttcttgga ctgatactgt aaataaatat acaaagagtg taatagataa
     4681 ggtgagaact tggatagagg aaaataatag aactaccaag attgcaggtg agaagaagaa
     4741 ttatcatgta ttttacaaaa ttgagtaaat ttatcagttg tattaaataa tttagtttag
     4801 ttttgggggg attaatacaa tgtgtgagaa tttacttgat aatatgtata ttgaaaagag
     4861 aaaataagaa tatagaatta aacttttgaa aataagagaa acagatatag atatatataa
     4921 taagttagaa agtatagtat ataaactttc tgagaaaaaa ttagagaaaa ataataaata
     4981 gacatataaa gcacttggat attctactgt ttcaagtgct ttgtttggta taaaatggta
     5041 taatggagat agagaattat attaacccaa aaatggagaa aatagagaag atgatttagt
     5101 tgacgcttta tcagtaggag tacttgcagg taagacagaa tctctagtac tattagttgg
     5161 aaatggaata agtgatactc aaaaaagttt aataaataaa agagaattta gaaatataac
     5221 tcaaattggt ggaaatggaa atgagaaagc atttagtgaa gtagaaaact tagttaaata
     5281 gattagaagg tagcaactag agttggttgt taccttcttt ttttatactt tctctctcta
     5341 agtattttct ttttcatatc ttctaataca tctaatgcag tagtataaaa ttgtatatta
     5401 tttctataag gtatagctat tggaagaatg ctcttgttaa ctaaaaaaat aagaagtaca
     5461 ataaataaaa gtgtcataat cattctaata aaataattag tgatatctga taaatcaaat
     5521 gttaagttaa ttgttgtaga aaatacaaca gccataatag atatatacaa agcaaaaatg
     5581 gggttactat ttttttgtaa ctgtccttta agcctagatt gctctatgtc tatatcttga
     5641 atacttttta tggaatccat ttcactataa tacttataaa cttttaaata atatttttta
     5701 tacttgttta aagcttcttc attatcttta aggcctttgt cacttaactc tgatttaact
     5761 tccccaaaat ccataaaatc cctcctacaa tgttatataa tatactttaa tttatagcat
     5821 atttaagaac ttatatcagc aatttgtcga acaattttag ctaaaaatag agtataatga
     5881 taggcttatt atttataaga tattattttt tatatagtta tatatttctt tctaattaga
     5941 taatataatg ctgttgttat aatggctact aatattattg ctaatattgt aatatctaat
     6001 cttcttgtaa ataagcctat tgctatatac tcaagacctg ttacatagcg ataaagtact
     6061 tctagtttaa cagttttttt cttatctata tttccatata ttgattctaa tttattaaaa
     6121 tattttttac tacaaaaagc agttttaggt ttaattaaat atattattgt agttcctatt
     6181 ataactatta ttccaataag tataagattg atatttccaa aatccatagc agatacctcc
     6241 tattaaattt gcttttctta atcaaagtat atcagtaaca caagtacaaa gagtatctaa
     6301 aatattacaa atattaacag gatagataaa actatagtaa aaattgtaat aataaagcga
     6361 acgaaaatta tcatactaaa acataagaca tgttataatt gtattagata gaagattaac
     6421 atacaccaaa gcactcctta taataaagag tgcttatttt tttgaaattc atcaatatat
     6481 aaactatcaa gaaccttatt caacacacct taaaatcgat ttaaatagct ctttttgata
     6541 acataattta tatgatataa taaaaaaata gaagtgtacc accaatacac ttctatagct
     6601 ataactaagc attctcttgc tgggggagtg cttttttcat ttctccaaac aacattattt
     6661 acttcatcag taattatcaa atcaatctca ttatctttca aaatctctgt tgcagtacaa
     6721 attagattat ctaagtcaaa acttcgagtt atctctagtt gtttgttatt tacttctctt
     6781 tttagtatat aagttttaaa atacatttat atcacccctt gtatttactc ttttttctct
     6841 aaaaatatat tgataagtaa atatgcaaca aataaaaaaa tctcaatgac acatagtgtt
     6901 atagctataa ctagaagtga ttgatttttc catgcactat aagctatgat gaaaagaaat
     6961 gtaaaagtaa ataatgtata attcataatt ttaactaaga tatgacttgt tttcattgtt
     7021 aagtacaagt ctctttcatc tgtactatcg tgtaattctt caacaatgtc ttttcttgaa
     7081 aaagcattac aaaaattaaa gattactatt acaattaaaa gaaatgatga aattaggaat
     7141 cttttatctt ttaaaatgca ataaataact aaactagcac aacataacac acaaactata
     7201 atacccacta taaaacttcg tttactcttt actttcatgt tttttatcct ccatttcttt
     7261 attttctttt aaacaacata gttcttcaat cgttgtacca aacacttgtg ccatacgata
     7321 ggcaagcata agagaagggc tatattgctc tttttcaata gaaataattg ttctgtttga
     7381 tacatggact aaatcagcga gttgttgctg tgttagaccc atatttgttc gaaattctcg
     7441 tacttttgtt ttcattccat aacctctttc tatatgaagc taacttcatg tgaagttaac
     7501 ttcataatat cctaaaatga gtaaaatgtc aatctcatta gataagtatt ataaaaccta
     7561 aaagaataat atggtaaaaa ttagatggaa ttatttacct taattctctt gtttgtaaaa
     7621 ctttattgat atagaattta acatatacat aaatcggcaa aggggcgcga attatgaaaa
     7681 ttagtaaatt accagaagcg gaattaaaag ttatgagata tatttgtgaa gctaacaagg
     7741 tattaacttc tagggaatta gttgctgcaa tggaagaaaa atatgagtgg aatgaatcaa
     7801 ctacatttac agttttgaaa agattagagc gaagggagtt tttaagtaca gagaaaatag
     7861 gcaaacttac acattataat atactagtaa aagagaaaaa atatctaagg tttgaaacaa
     7921 aggaattttt gaagaacata cataaaaatt caatttcaag tttaatgtca gcgttacatg
     7981 gtgaagacga cgaagtggat gaagataaat taagtgaact cgaagaatat tttaagaatt
     8041 tgaaataaga aaatgacaaa ttgatacata ataaataaaa tgaaggtatg aaattataac
     8101 tttttctacc ttcattttgt ttattattct gaa
//
LOCUS       DAELJO010000165         8027 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELJO010000165 DAELJO010000000
VERSION     DAELJO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.167"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..773)
                     /locus_tag="KPB88_003040"
     CDS             complement(<1..773)
                     /locus_tag="KPB88_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0055299.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDE"
     gene            complement(1099..1950)
                     /locus_tag="KPB88_003041"
     CDS             complement(1099..1950)
                     /locus_tag="KPB88_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0055300.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1999..3168)
                     /locus_tag="KPB88_003042"
     CDS             complement(1999..3168)
                     /locus_tag="KPB88_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0055301.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            3639..4556
                     /locus_tag="KPB88_003043"
     CDS             3639..4556
                     /locus_tag="KPB88_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0055302.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(4685..5614)
                     /gene="pfkB"
                     /locus_tag="KPB88_003044"
     CDS             complement(4685..5614)
                     /gene="pfkB"
                     /locus_tag="KPB88_003044"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0055303.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            5881..6606
                     /locus_tag="KPB88_003045"
     CDS             5881..6606
                     /locus_tag="KPB88_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055304.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            6689..7843
                     /gene="nagA"
                     /locus_tag="KPB88_003046"
     CDS             6689..7843
                     /gene="nagA"
                     /locus_tag="KPB88_003046"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0055305.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
BASE COUNT         2780 a         1206 c          989 g         3052 t
ORIGIN      
        1 ttttcatcat aattaaggtt atatttttta tctctaaaaa ttttatctaa taatttatct
       61 ataattaaat cttctgtata taattttaaa tcgctagcat ttcccgctac tctcatacta
      121 actcttatat acaatataat aatttgtgct aatatttcct caagttctgt taaatttgct
      181 tctaaatcat gttcattaag tacatttttt attgtacttc taatattagc taattttaaa
      241 ttatctataa acttattcct gtactctgtt atatttctat tccctctaaa ataactatcc
      301 accaatatac tttgaatttc ttttatagaa ccttctattt taattccata atgagccttt
      361 cgctctagtt ttaattctgt atcctttatc ttttcatcta tcttttttaa atcatttttt
      421 atttgggatg aacttactag aaatttttta cttaactgtt ctatagtttt ataaccttca
      481 tttactaaaa gctctactat tatagactcc aaccgttgtt ctggattttc aactaaatct
      541 gagggtaagt ttcttagata atgctcgaat ttatcttggt ctttaatatt tagaatatat
      601 cctaatttag acttatattc taatttaaaa ccattacatt cttttatagt ttctatcttt
      661 tttatttcat ttctgatagt tcgagtagat acccccaact tatctgcaaa ataatctatg
      721 tttagataac cactactatt ttttaatgtt tcaagaataa tgcgttctct catatcccca
      781 cctccctcag tgttattatt gtgtttttta aagtggtata aaaaatattt tttataaaaa
      841 caaccttttc tttttattta agaattttat atcctagtat attttaattc cacaatttaa
      901 tataaatatt ttataataat aaaatatata gttcaatttt tagtatattt taattatata
      961 tgtaatactt taccaataaa agctgatatt tttcatcaat taaatgaaaa aaatacacca
     1021 tttacatata attaatataa atttttcata taacaaatat cctaaactta tctaaaataa
     1081 atttaggata ttttattttt atactctatt tacagaccca cacactttaa tcttttcttt
     1141 cacaaaatcc tttactgatt gaagccctgc taatccatac tttttagggt ctataacctc
     1201 agaatcttca tctaaaactt ttttaacccc tttactatat gcaattctta attcagttgc
     1261 atagttaact tttgcaatac ctctttttat agcttctttt ataatatcat catgtattcc
     1321 tgaacctcca tgcaatacta aaggtattga aactacattt ttaatttctg ataacctatc
     1381 taaatctatt ttaggttcac ccttatacaa tccatgtgca gtacctatag caatagccaa
     1441 tgaagatatt cctgttctat cgacaaactc ttttgcctct tttggatttg tataatcatt
     1501 actattcccg ccatctaaat catcttcttt tccaccaact ttcccaagtt ctgcttccac
     1561 tggtataaga gaaggactac atgcttttac tactgattta gatatagata tattattttc
     1621 aaagctttca tgagaaccat ctatcattat tgatgtatat ccacttctta aagctttcat
     1681 tgcaaggtca aaacttgaac catggtccag atgcattgct acaggtatat ttactctttc
     1741 tgcagcaacc tttgcatttg ctagataata atcaagacct gcatatttta cagtagatgg
     1801 agtagtttgt aatattactg gagaatttaa ctcctcagca gcttctataa cagcaattac
     1861 catttccata ttttctacat taaatgctcc tactgcatac ccttcatctt gtgccttttt
     1921 aagcattgat gttgttgtta ctaatgccac attgataccc tcccaaatta tttagttata
     1981 atattttaat cttatctttt aatattcata tattgttaca ccttttacta ctcgatttac
     2041 tgttcctgta gctgatggag tatctggctt atttccaacc tttaccgata caaacaatgc
     2101 tatagtttgt gcaaacaata tgtatggcat tgcaagatac acatctggta atttattata
     2161 cttttcttta aactcaaact ttgttcctga gaagttattt tcattttcta ctgacactgc
     2221 acaagtgaat ttagcaattt tatccctttt tatctcctct aaaacatcta aatcatattt
     2281 tcttgtatat aaacagttag atacaaattc aaatacaagt gtattttcat caataaatga
     2341 ttttggtccg tgtctaaacc ccataggaga atcatacact gtagatattt ttcctgctgt
     2401 aagttctaaa agttttagtt gtgcttcttg tgtcaaacca cctaatcctc ctgaacctaa
     2461 atatacaact ctatcaaaat ctttatttat taactcatgt atttcatctt ttctatctat
     2521 aacatttctt cccatttcaa ttattgcatt tatatatgac ttttctacat catcttctaa
     2581 actatcgaat attaacatag cagataacat catacacgaa aaacttcctg tcatggcaaa
     2641 gccttcatca tttgattttg atggcattaa taacaagtaa ttattttcgt cgtttttagt
     2701 catcttagcc agttctcctt ctggattaca agttatagct aaatgataaa aattatctac
     2761 aatcttattt cctaaattta aagcagccaa actttcagga ctatttccac ttcttgcaaa
     2821 tgaaattaat aatgttggta tatctttttt taagtagtca tatgggtttg aaactatatc
     2881 cgtagtaggt attgcttcaa atatgtattt tcttatgtca tttttatttt taaggtaagg
     2941 aagtattgaa tttcctatgt aagctgaagt tccagcacct gtaaatataa ctctaaactg
     3001 cccatgatta ttacttattg tatctatgaa tctacttaat ttttctttgt tagatttata
     3061 tatttcataa gtttctaacc ataattcagg ctgttgtttt atttcattag ttgttattat
     3121 tgcacctaaa tcttttagtt tttgttcttc taatttaaac attttcattc tcctttcata
     3181 cttgtaacgt ttacatttgg taatgaggtt ataaccactt taaactataa aaaaatatac
     3241 tcttttataa ttttttgctt agatttttta gtactactta aactcactat aaaatataca
     3301 ctatagattt taattaattt tatagtttat ctttaaaaat ttaataaaat catactctat
     3361 atactttagt atttttattt tacgataaaa acttgttcag accaaagtgg taattaccac
     3421 taatccaaat ataaattaat ttgaattttt tgtcaataca taaaatattt atatcaatca
     3481 attttatgga tatcctacaa aattacttta tcaatatttc ttatttcgct aatttactta
     3541 tataagcttt aaatttattt tttgatacaa agtttacaaa tttatgtgtc tgaattataa
     3601 tcaattctgt aaatataaaa accttaggag aaagtattat gaaaaatata aaaatgggaa
     3661 tgataggtct tggctccata gctcaaaagg catatttacc tatattaact aagtctgaga
     3721 gatttgagtt tgtaggagct tttactccaa ataaagttaa aagggaaaaa gtctgtagtg
     3781 attatagaat catgccattc gattctattg aatccctagc taaaaaatgt gattgtattt
     3841 ttctacatag ctctacagaa acacactacg aaattataaa aatattatta aatttaggtg
     3901 ttcatgtata tgtagacaaa cctcttgctt caacagtaag tcaaggagaa gaacttatcg
     3961 aattaagtac gaaaaaaaac ttaaatttaa tggttggatt caaccgtaga ttctgtccta
     4021 tgtacaaaga aattaaaaat aatgctacag aaattgtatc tatcaatata tgtaaacatg
     4081 gattaaattc cttaagaaat gttaaattcg acagtactct tatagatgac tatatacatg
     4141 ttattgacac tgctttatgg ttagcaaatg aagatgttga aattagtgga gaggatttat
     4201 ttttaacaga caataagaat cttatatttg taagtcataa acttaaaggt aaaaatttct
     4261 ctataaacac ctctatgcat agagattctg gaacaaagtt agaacaagtt gaaattcttt
     4321 caaagggtaa gattcaaaga gttaagaatc ttaatgtact ggaaatagaa gaaggtggaa
     4381 acctaacttt aaagcaaagt ggtgcatggg taaacatatt aaaacaaaag ggttttgagg
     4441 atatatcaaa tcattttatt gattgtatag aaaataatat aaaaccagct attaatggag
     4501 aagagtgtat caaggctcaa agattactag agaaaattat aaattcagta aaatagatat
     4561 ttattataaa aataggttaa aaatgaattt ttctaaccta tttttatata agactatatc
     4621 ttttatatta aaattaaaat ctattacaac aacattaaat ttattatatt cccattaaat
     4681 gtatttaact taactttttt acttcaacta aatcttgata tttatcaatt tctaccaaat
     4741 ctacaaatcc agtttccata tttaaagcat tacttatacc acaagcattt gcaaatttaa
     4801 gtaaattttc tattttataa ccccttaaaa tacctacact aaatccagcc actgtgctat
     4861 ctccactacc tacagtattt atacacttta caactggtac ttttacatca tatacattat
     4921 cttcgtttaa atacaccata ccatcttttc ctaaagatat acatacattc tctgcaccca
     4981 tttcaataag tttttttcct gacaagataa tttcttctct tgattctata ttcattccta
     5041 ataaatgtct tatttcatct atatttggct ttattaaata tggttttgat tttaaagcta
     5101 tttttaatgc ttctcctgat gtatctaata taaactttat attatttgct ctacatatat
     5161 ttcctatctt ttcataatag tctatcggca tattctcaca ataactacca gaagctacca
     5221 gtattttagt attttttagt acttcctcta tatcattttc aaatctctct aaatcgcttt
     5281 tttctactat tggtcctttt tctaaaaatt ctgtactaac tttattgtcc tcaattatgt
     5341 ttaagcaatt tctagtttcc tgctctattt ttgtaaattt attttgtata tctagttttc
     5401 taagttcatt ttttataaat tctccattaa atccgcctag aaatcccata gctaaaggat
     5461 tttcttttaa aattttacat accttagcta cgtttaatcc tttacctcca gcagttgcat
     5521 tcttacttac taccctttgc acctcaccta tgtttatatt attcactcta tacattctat
     5581 ctatagatgg gttgaatgtt attactgtaa tcatactaga ttctcctcat tattaatttt
     5641 tacttttcta aacaataaat taatctgtaa aataaaaaat acctcatata atatatccaa
     5701 agtggttatt accaatttga atataaatta ttttacttgt tttgtcaatg taccataatt
     5761 ttataataaa ttaatagcct tacttattga attttgatta aactttattc attgacataa
     5821 ataattctat attctataat caaattgtgg taataaccac ttagaaagga ctatattttt
     5881 atgataaaaa gaaatgataa aagaccaata tatgaccagc ttgttgagat attaagaact
     5941 aagattgaaa atgagatgga accaaacgat agaatgcttt ccgaaagaaa aatttgtgat
     6001 gaatatggag taagtagaac cacagttaga ctagctatgg ctgagttaga gcatatggga
     6061 tatatatata aaagacatgg aaaaggaact tttgttgctg cactaagtca aaattctcaa
     6121 aacttaatgg aaagttatag ctttacagat tatatgagaa cacaaggaaa aaagccaggt
     6181 acaaaggtat tatcatttga ggtagtagaa agtacaaagt atttctctga aaaattaggt
     6241 ataactcctg gtgaaaaaat gataaaaata atgcgtttaa gacttgctga tgaccttcct
     6301 atgatgcttg aaagaacata tcttcctatg aaagaattta taggattgac aaaagaaaaa
     6361 atcgaacaaa aacctttata tgaaatattt agagaagatt atggcgagat tattaaagtt
     6421 gctgatgaag aattttcagc tggaataatc tctgataagg aagctaagtt attggaagta
     6481 ccaggagatt caccttgtct gaaattactt cgtaccacat ataatgatga caatatagtt
     6541 attgaattta ctttgagtgt cgctagaagt gataaatttg tatatagagt aagacatgta
     6601 agatagtaag ttaaataact ttcaagaaaa cagaatgata ttataagcgt caatgccaca
     6661 atgatagtat aattataagg aggttatttt gaaaagtttt atttatgcag ataaattttt
     6721 tatgaaacat ggaatcaaag aaaaaggtta ccttagtata attaatggca aattcggtga
     6781 atttcaacaa gaaagaccta atattactga taatgttata gatttttcag gtaaatatat
     6841 tgctcctggc ttagttgata ctcacattca cggtctttta ggtgcagatg tcatggacaa
     6901 tacatttgaa gcaataaaca caatttcaaa aggcttatta aaatatggag taacatcttt
     6961 cctacctaca actttaactg attctgtaga tacactaagt gattctgtag aaaatataag
     7021 caatagcttc aatcgagttg agggtgcaaa aatacaaggt atttatttag aaggtccatt
     7081 ttttacagaa aaatataagg gcgctcaaaa tgaaaaatat tttaaagacc ctgacatcaa
     7141 tatattaaaa ttatggcaaa aaaaatcgaa aaatttaata agaaaaatag caattgctcc
     7201 agaacgtaaa ggttcaatag aattaacaag atatgcaact aaaaataata tatcagtttc
     7261 tcttggtcat agttcagcta catttgagca agttaaacaa gtcgttaatt caggtgctaa
     7321 agtgtttgta catacatata atgggatgag tcctttaaat catagagaac ctggaatggt
     7381 tggggctgct atggtactaa aagatactta tgctgaatta atttgtgatg gtcatcatgt
     7441 aagcccaata gctgttaaaa taatgatgga tgctaagtct agtaataaca tagctttaat
     7501 tacagattgt atgagagctg gtgctatgga ggatggaaaa tatactcttg gtaagtttaa
     7561 tgtaaatgta aaaaatagtg ttgcaagatt aaatagtggg agtttagctg gaagtgtatt
     7621 aactatggac aaggctgtga gaaatatagt tgattggaac atatcaactt tagaagatgc
     7681 aataaaaatg gctacatatg tccctgcttt aagttgtaat attgataatg tctgtggctc
     7741 tatatatcaa ggtatggatg cagactttat agtaacagat aatgacttta atatacacga
     7801 aacttatata aatggtatct gtaagtacaa agttaataaa taggttcaat ataaggttat
     7861 ggaggctgtc taaaaagagt ctggaaatat ttaaagttat ttctccaaaa tctgatttaa
     7921 cacttttatt tccatatcgt taataaacta ttgcgataga ggttatttta aacaaatata
     7981 tattataagt atttttgtga taatattttc gataatatgt acattta
//
LOCUS       DAELJO010000166         8012 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELJO010000166 DAELJO010000000
VERSION     DAELJO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.168"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            226..3174
                     /locus_tag="KPB88_003047"
     CDS             226..3174
                     /locus_tag="KPB88_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0055306.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3152..3616
                     /locus_tag="KPB88_003048"
     CDS             3152..3616
                     /locus_tag="KPB88_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055307.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEMDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVNLSEESVSDLLNSKLKN"
     gene            3729..4031
                     /locus_tag="KPB88_003049"
     CDS             3729..4031
                     /locus_tag="KPB88_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0055308.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4093..5457
                     /locus_tag="KPB88_003050"
     CDS             4093..5457
                     /locus_tag="KPB88_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0055309.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVIFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5492..5965
                     /locus_tag="KPB88_003051"
     CDS             5492..5965
                     /locus_tag="KPB88_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055310.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINNGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            6137..6787
                     /locus_tag="KPB88_003052"
     CDS             6137..6787
                     /locus_tag="KPB88_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0055311.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECSSTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6961..7893
                     /locus_tag="KPB88_003053"
     CDS             6961..7893
                     /locus_tag="KPB88_003053"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0055312.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKYNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFDNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT         3297 a          851 c         1368 g         2496 t
ORIGIN      
        1 ataacattat cattttagaa tgatttcaat tgtaattagg aaaagtgatt tttttgtttt
       61 taaaaaaagt attatttgta aaatataaaa aaaaatttct taatatttga ataaaagaat
      121 agcttaattt aaatgttatt actttataaa aaaataataa gtacaaaaaa aacaatatat
      181 ggcatataag ttgctacata aataataagg agggaacaat tataaatgag gcaaagaata
      241 aaaaacttaa ttgaaaatga agataagaaa aatcctttaa cagatgaagc gatttcaagt
      301 aggttaaata ttcgaagaga agatgttacc tttttgagaa atgagttaaa aatagaagat
      361 tcaagacaaa gaagaaaagt tgttttaatt aaagctataa aagatatttt aaaagaagaa
      421 aaaactataa ataaaaatga tataacaaga agattaaaca gcattgggtt tacagtatca
      481 agatttacag tcatacaata cttaaaagag attcaagatt ctggtgacct aaaacttgat
      541 aaagagagtg atgataaaaa agaagaaata atcaataata gagaaaataa agatatagca
      601 tttgataaac tcataggtag tagtggtagc ttaagtacaa ttataaaact agccaaagca
      661 gcaattcttt atccacctca tggtcttcat actattatta tggggccaac tggtgttgga
      721 aagagtgagt tagcagagtg tatgtataaa tttgcagtag aatcatccag attccctaag
      781 aatgcaccat ttatagtttt taatgcagca gattattcag agaatccaca gcttttgcta
      841 tcacagttat ttggacacgt taaaggtgct tttacaggag ctgatgagaa caaagaagga
      901 ttagtatcaa aggcagatgg aggaatattg tttatagatg aaatacatag attacctcat
      961 gaaggtcaag aaatactatt tcaactgatt gataaaggta tggtcagaaa gttaggagaa
     1021 acaagactaa cacataaaat agatgtaatg attatttcag caacttctga gccaatagat
     1081 tctcatttac ttaatacatt taaaagacgt atacctgtaa caattgaaat accagaatta
     1141 gttgcaagac cattaaatga gaggtttgac attataaata atttcttctt aatagaagct
     1201 caaagaatgg gagcaaacat acatataaag tcagatgtat taaaagctct tatgctttac
     1261 gattgtatag gtaatgtagg tcaacttaga agtgatattc aagtagcttg tgctagggga
     1321 ctattaaacc aacttacaaa caaactaaaa gaagtaaata taagcatatc agatgtacct
     1381 ggatatgtaa aacaaggtct tataaaaatc agaaattgta gaggaaaaat agaaaattat
     1441 gtagatgggg atttaattat agactcaaat cttcaaggtg agatagaaaa gaaaaatgaa
     1501 gatatatata cttttccaga tgaaatatac aaacttactg aaagaagaca tattgattta
     1561 ttggaacaag gtttagattt tgatgttata aatagaatca ttggaggaga attagaaagt
     1621 tgcattcaaa aatacataaa acaagttaaa aaagtttcta ttagtaacaa tcttccagat
     1681 ataagtccaa ttgttggaaa atatatcgta gagctaactt atgaagtaat caaaatagca
     1741 tcacaatacc taagttcact agaaccgaat ctacatttat gtttagcagt tcacctaaaa
     1801 gcagcttttg agagattaaa agaaggaaaa gtaatatcaa atgctcattt gaaagaaata
     1861 ataggaaatt atccactaga atattctgta gcagaaaagg tagctcaata tttaaaggaa
     1921 agatataaca taaacttacc aaaagaagaa ataggtataa tagcaatata ttttaaaatg
     1981 agtagtagaa gagatatgtc tgaacaaaaa aagataggcg tacttcttat ggctcacgga
     2041 agagtagcat cttctgtatg tgaggttaca aataaactat taggaattaa tcacgcaagg
     2101 tatttagata tgccatttga gaagaagccg gaagaaatga ttgaagaagc tactacaata
     2161 ataaaagata ttgatgaagg tagaggtgtg attatattag tagatatggg ttcactttca
     2221 tattttggag atattatttc tggaaagact ggaattaata taaggacaat atctagattt
     2281 gatacattgc tttctataga ggttatacga agagcagttt taccagattt aacaatagac
     2341 gagatagttg atgaagttaa aggcatagag ccacaagtaa aagaaaaggt tagtaaatgt
     2401 aacaatacaa aagtagattt tagaaaacct ataattttaa cattatgtat tactggatta
     2461 gggggagctt gtaagataaa aaaaatctta gaggaaaata ttaaaagtat aactgaaaag
     2521 gttgatatag aacctatagg tatgattgaa tatccaagtc ttgatttaga gataaaacaa
     2581 ataagcaatg ataaaaatgt aatcgccata gttggaacaa ttgaccctca gatagcaaat
     2641 atcccttttt ttagtctagt agacataaat gataaagcaa agttaaatca aataaataac
     2701 ctaattgata caaatcaaat tggtgaaaca aataaaaaat atgaattatc agatttaata
     2761 cataaagaag atatatttgt ggatttacag gttaaatcaa aagaagaagt tctaaaatat
     2821 atgagtaaaa aacttagaga gaaaggttat gtgacaaaaa atttctataa aaaagtagtg
     2881 gaaagagata acttatttcc aactgaatta tcagatgaaa tagcaatacc tcacacagat
     2941 ggaatagatg taattagacc agctataagt atagccatct taaagaagcc tattgtgtgg
     3001 agtaaaagta aagtaaaaat aatactttta ttagctgtag accaaaagtg ttttaatcca
     3061 ttaagcactc tactctcatt tatagaaaca gatgaattta agaagataaa aaacattaaa
     3121 gatagagatt ttattaggga gatgatatta gatggagtca atgaaaataa ttagtagcaa
     3181 tttaattttt aaaaatattg aagtgtctaa caatgaagat gcattaaagt ttttgggaca
     3241 acgtttgttt gacgaacagt atgtaaaaga aagctacata caagctgttg tagcaagaga
     3301 aaaaaagtat gctacagggt taccaacaga aatatacgga gtggcaatac cacatacaga
     3361 tatagtacat gtaaatgagc caggaatagc aattggaata ctgaataaac ctgtaaaatt
     3421 tataatgatg ggtacagacg atactgaaat ggatgtaaaa gttgtattta tgttagcagt
     3481 gaaagagcca caagaacaat tgcaactatt agaaaaactt atgactatat ttcaagataa
     3541 aaatatacta aataatattg ttaacttaag tgaagaaagt gtgagtgatt tattaaactc
     3601 taaattgaaa aattaaacat gtaaagtata ttaaaaatgt aaaattaaac atgcaaagta
     3661 tgttaaaaaa tataaaaaga ttattaaaga tttctaacta tagatatctt taaaataaaa
     3721 taatttcaat gggggtgact aatgtggcta aaaagatatt agttgcatgt ggaacaggag
     3781 tatgtacttc aacaattgct ataaacaaat taaagaaagc acttcaagac attggtaaat
     3841 tggatatggt taatattaca caatgtaaag tagtagaagt tgcatcaaaa gcacctgatt
     3901 atgatttaat aatttgtact acacaagtat ctagtagtat aaaaacacca gtgataaatg
     3961 gattaccttt tttaactggt gtcggaatgg ataaattaat caatgatgta ttagaagagt
     4021 tagaattata attaaaaaaa ttaaggaaca tggtctctaa aatcataagt tttagagact
     4081 taaaattaaa taatggaggg tctttatatg gagatattaa atttcattgt tggtctaggt
     4141 gcacaagtaa tgatgccaat aataatatgt atttttggtt tgatattagg tacaaaacta
     4201 ggaaaatctt taagagctgg tttaactgtg ggagttgggt ttataggtct taacactatt
     4261 atagctttgc taacagacaa tttaggtcca gctacacaac aaatggttaa gaatttaggg
     4321 ctaagtttat caataataga tgtaggatgg ccagcagcat cagctatagc ttttgcatca
     4381 acagtagggg ctttaattat tccaataggt cttgtggtaa atatagttat gctaattact
     4441 aatactactc aaactgttga tgtagacata tgggattact ggcattttgc ttttacagga
     4501 gctttagtgg caggggctac tcaaagtgta atgtggggag tatttgcagc agtagcaaat
     4561 atggtaattg tattggtaat ggcagattta actgctccag gtatagagga atatttaggt
     4621 atgccaggta tatctcttcc tcatggattt acacaggcct ttgttccaat tgctatagta
     4681 gtaaataaat tattagacct aataccaggc ataaataaga ttgagataaa tgctgataca
     4741 ttgcagaaaa aatttggatt atttggagaa cctttaataa tggggtctgt aataggtgta
     4801 ataattggta ttgctgcgaa atatgatata aaaggaatat tacaattagg tgtaactatg
     4861 ggagctgtat taatattaat tccaaagatg gcagcattat taatggaagg attattacca
     4921 gtatcagaag ctgcacagga atttattgag aagagattta aaaacagagg caagatttat
     4981 ataggcttag attcagcagt aggaataggt catcctgtta cattatcagt tgcactagtg
     5041 ttggttcctt tgactatact tatagcagct atattgccag gaaataaagt tttaccattt
     5101 gcagatttag cagtaattcc atttgcactt gtattaatcg taccaattac aaagggaaat
     5161 gtgtttagaa cattaataat aggaataatt ataattactt ctggattatt aatcgcaact
     5221 aacttagctc cattatttac ccaaatggct ttaaatgcta gttttaaaat gcctgaagga
     5281 gcaactatga tttcaagtat ttgtgatggt gcgaatccat taagttgggt atttgtaaaa
     5341 gttatgaact ataaagttat tggaggagta atttttggag ttatagcttt aggaatggct
     5401 atttacaata ggaatagaat aataaatgaa aataaaaagc tatctataga agaataaggt
     5461 taaaacaatt ttattaagga gtgtataggt tatgaaaaaa tataaatatg attctaaaag
     5521 gtacttactc agtgttacaa taccaggatt ttttatgatt ttaatattga tttatgcact
     5581 gtttaataac tatacaaatt ttaatttgaa tatatatagt ttgttaataa ttgtctgtac
     5641 ttatggtata ttcaatactt ttatatcatc aagtaatcct aaaaaaatta ttattgatag
     5701 caaatgtata cattttacct catttatgac tacacataag tatgaaatta aagatataga
     5761 aaagataaga ataagagaat tttataataa aaaaatttat ctaaaaatta ataatggaaa
     5821 cctttttaaa ggtaggtatt ggataagaac aaatatgttt aataatagta tagcattata
     5881 tagctatttc attgagttgg aagagtgctt atatccagat tcactaaaat ttagaaataa
     5941 aaaatttgag aataagaaca tataaaaata aaaaattgaa ttgaagccta gcttaatctt
     6001 ttttgataaa atataagttt gaactttata ctaaattttt agaattttca atcaagacaa
     6061 taacataata gctaagcaat tagaacacaa ttaagatgta taaggcatgt atatgatgaa
     6121 tgggaggatt taattaatgt tattagagaa attaagaaaa gaagtattac aagcatcttt
     6181 agacctttta aactataatt tagtgacatt aacaggagga aatgtaagtg ggagagatga
     6241 acaaactggc tatatagcaa tcaccccaag tggaatggat tatagaaatc ttacaccaag
     6301 tgatattgtt atagtagatg tagatggaaa tattatagat ggaaaatgga aagcatcagt
     6361 ggatttatca gaccacttgt atatatataa gcatagagaa gacataaata gtataattca
     6421 tacacattct acatactcaa gttgttttgc tattttaaat gaacctatag aatgctcaag
     6481 tacaactctt gcaaatgaag tagggggttc agtaccagtt gcaaaatttt caccaccaac
     6541 atctaaaaag atgggaaaat gtgttattga agcaattggt gacaaaagag catgtctttt
     6601 agcaaatcat ggcgtaattg cagttggtcc atctgtagga catgctctta cagccgctgt
     6661 aatgttagaa gattcagcaa aagtatatta cttagcaaaa tcaattggca caccagtgct
     6721 actgcctgat gaagaaattc aaagagcgag agatgtattt tttaatgtct atggtcagga
     6781 taaataaatt agataaaatt ttaaaagtaa tatgaaaata tagatattca atgatattaa
     6841 ggagatttat aatgatataa taagtttaat agttaagaag taaaatatga aatgtaatag
     6901 tatataaatt catttaagac taaaaataat aagataaaat aaaaaggagt ctgtatttct
     6961 atgtattact tattaaacaa aagcacaaat ccttacttta atttggcatt agaagaatat
     7021 ctatttttaa atgataaata taatgatgat attataatca tatggagaaa tgaagaatcc
     7081 atttttatag gtaaaaatca aaatccatat caggaagtat atcatgatgt tattgaaaag
     7141 ggagaaattc ctattttaag aagaatatct ggaggaggaa ctgtttatca tgacttaggt
     7201 aatatcaata tgtcattcat acaaaaagat aggcaacttc atgagataga ttttttggag
     7261 catactaagt ttatgcaaaa gatgctttca accttaggac tagatgtatc tataactgaa
     7321 agaaaagatt tatttttaaa tgggaaaaaa atttcaggtt ctgcacagtc tataaaaaga
     7381 aaaaattctc tttatcatgg aactcttctt tacgattcag atttaaataa attaactaaa
     7441 tatttaaatt caaataaagc aactgaatca aatgcaacta aatctgtttc aagtaaagtt
     7501 acaaatatta agtttttact cgaaaaagat ataaattact ttttagatta ttgtgttgaa
     7561 tatttaaaat ataatataaa gaatataaaa gaacttgaat tggatgaaga agatattaaa
     7621 agtgtataca atataggaga agataaatat aaaaagatag agtggacata tggtaaaacg
     7681 ccgaaatttc aagtagtgct accatttgat aatgaaagta aaataaatat acatgttaac
     7741 agatggaaag tatcaaaatt ttttatttct tatgatgata atgtaattaa tatggacaag
     7801 ttgttagatt tgaatttctt taaagaagaa ataaaagaga gtattttaga aaattatcca
     7861 cattacatag atttaatcaa tttaatattt taatataaaa cctctaattt gataaaattg
     7921 tcttatatca aactagaggt ttttaatatt cttgcatatt aacaaccatt acaaaattgt
     7981 aaaattaaaa acaacactat tgaaaaatca at
//
LOCUS       DAELJO010000167         7985 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELJO010000167 DAELJO010000000
VERSION     DAELJO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.169"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            115..1509
                     /gene="yfcC"
                     /locus_tag="KPB88_003054"
     CDS             115..1509
                     /gene="yfcC"
                     /locus_tag="KPB88_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG0055313.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            2026..3393
                     /locus_tag="KPB88_003055"
     CDS             2026..3393
                     /locus_tag="KPB88_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG0055314.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            3418..4026
                     /locus_tag="KPB88_003056"
     CDS             3418..4026
                     /locus_tag="KPB88_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG0055315.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            4074..4445
                     /locus_tag="KPB88_003057"
     CDS             4074..4445
                     /locus_tag="KPB88_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG0055316.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            4624..4962
                     /locus_tag="KPB88_003058"
     CDS             4624..4962
                     /locus_tag="KPB88_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG0055317.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            5429..5932
                     /locus_tag="KPB88_003059"
     CDS             5429..5932
                     /locus_tag="KPB88_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG0055318.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            5979..6938
                     /gene="moaA"
                     /locus_tag="KPB88_003060"
     CDS             5979..6938
                     /gene="moaA"
                     /locus_tag="KPB88_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG0055319.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            6942..7412
                     /gene="moaC"
                     /locus_tag="KPB88_003061"
     CDS             6942..7412
                     /gene="moaC"
                     /locus_tag="KPB88_003061"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG0055320.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            7448..7879
                     /locus_tag="KPB88_003062"
     CDS             7448..7879
                     /locus_tag="KPB88_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG0055321.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         3208 a          724 c         1447 g         2606 t
ORIGIN      
        1 ttttatttta tatctttgct aataaaaaat tgtaaaaagg gaaaacttta gtcagagaga
       61 ttaaacaata tcctagaaag atttaaaatt tagtaaagta tggaggacat tataatgact
      121 gacagtaaaa agaaacgatt tattatacca cacactttta caataatatt tttactaata
      181 gtcttgatgg caatattgac ttggatagta ccaagtggtg aatttagtag agaagatgta
      241 gaaggtagaa tggtagttgt accagggact tatcaaagtg tagaaagtaa tcctcaagga
      301 tttgcagatg tttttaaagc accaatagaa ggctttatcg attcagctga agttgtaggt
      361 tttgttttaa tagttggagg tgcttttggt attgtaaata ggactggagc tatagaagca
      421 ggaatagctt cggttatctc taagtttaag ggaaaagaac ttcttataat tccaatatca
      481 atgattttat ttggacttgg aggtacaaca tttgggatgg cagaagagac attaccattt
      541 tatatgatat ttgttccact catgacatcc ttgggatatg attctctaac agcaatagca
      601 attgtattta taggagcagc agcaggagcc gccgcttcaa caataaatcc attttcagtt
      661 ggtattgcac aggcactagc tcaacttgct cctggttcag gagtaactta cagagtagtt
      721 atttttatag catatatact aataagtata gcatttgtta tgtactatgc taagaaggta
      781 aaggaaaatc ctgaaagttc aatagtatat gaaatagata aaaataataa atcaatactt
      841 aatcaaactt caacagaaat aaaagaattt acttttaaag aagtatctgt tcttgctata
      901 tttggaatag ggatggcaat aatgatttgg ggtgttttaa gtcaaggatg gtacattcca
      961 gaaatatcaa tgtgttttgc tgcaataggt gtgctttctg gattggttgg tagattatct
     1021 caaattgaaa tctcagaaag ttttatagat ggagcaaaag atttagcatc tgcagctctt
     1081 gctatagctt tggctcgtgg aatcgtaata attgctcaaa atggatttat aatagatact
     1141 atattaaatt ccgcagcagc ctttctaggt gggcttccaa aggttatatt tgtatattta
     1201 tcatttttct tggaagctgc attggcattt ttaattccat catcttcagg tcttgcttca
     1261 cttacagtac ctgtacttgc tcctcttggt gatttggtta atatatcggc tcaagtgata
     1321 gtaaccgctt atcaatttgg tgtgggtctt acaaacttta taactccaac ttcaggagta
     1381 ctgatgggag cacttgcagt agcccatatt ccattttcaa aatgggtaag atttataatg
     1441 cctatcttgt tgattttgac agctgtatct ctagtctttt taacaatagg tatttatata
     1501 gggttttaat taagtgtagt tttatttaag atatttagta atggtataaa atgataatat
     1561 gttttcaata aattgccact aatttaatta gtggtaattt gttttttgta tttaaaaata
     1621 aatgtattta cttgtatttt gaattaatat ctacttagac attgttttaa ttgttatatt
     1681 tttatttaat taagcattga tttatcattt tttatatttc atataacaat gcaaaaaata
     1741 atttttatat agtaaatgac aaaagtttac aataaatgta tattttctta ctcattcctt
     1801 aaaattactc atttagttta ataactctca tttcatatgt aaagttggta aaaaaattaa
     1861 aattaaaagt aaaaaactaa ataaattaat gttaatttga aatatatatg aaggtttttg
     1921 gagaatactt acttatgata ggaaaaaatg ttaaaataat agtgtaatca aatataaagg
     1981 actgaaataa ttaacagtta ttaaatatta ggagtgtaaa aatttatggg attaaaagaa
     2041 tttgatttta ttgaagagtc tattgatatg ttgaggacaa tgtcaccaac attagaaact
     2101 atatcagatg aaatagaaga atattttgaa aatatcttgg atgaaaagaa tcaagaatat
     2161 ataaatgtaa cctctagaat aaaatcagaa tcaagtttaa gagaaaagat aattagaaat
     2221 agatatttaa aaaaatatgg agaagcaagt aatcttattc ataatgtttc tgacttaata
     2281 ggcctaagaa ttgagtgtag atttattgag gatgaaaaca aaatatatag acttttgaga
     2341 agatatttta ataaaacaga tgacaaaata aactattata ataaagagaa taagaatatt
     2401 aaattaaaac tatctgagag gcaacccaat aagcaaaaaa atggatttga aatatataaa
     2461 atagatggag tttttgcgta tttagataga gaagtaaaat ttgaacttca aattaaatct
     2521 cttgtaaatg tgttttggag tgaaatagaa cataaaataa tttataaaaa caatacatat
     2581 ttacttgctg ataaatttat aaaggatatg atggactcta taaagaataa tcttactatg
     2641 atagataatc agcttttaag tatttataaa aactttcatt cagggaagtc ttttaatatg
     2701 aaagtaagta aaaaagaaat tgaaaaatta tttgcgaaac tagtctatga cgctttttct
     2761 gaaaagatga ataaaagtat aggttttgtt gtagatttta aaaaaccatg tgagactata
     2821 ttgaactatt cttttaataa acaagatata agtgatgaag ttttaggtaa ttttatgctt
     2881 gatgagtttg caagattaaa tgaaattgta aacaaggata tagattttaa tgagcaaata
     2941 gaatttgaaa gagaaccaac atttgatgat aagttttgta aagacctagg aaatcatttt
     3001 agaagtagat taaacacaga atttccatgg aacctatttt ttagaatatt atttgaaata
     3061 gagccatata acaataccaa agactttgaa aactttgtag agtttataaa agaaaatgtg
     3121 ctactagaag aaaataggga gcaattatta tgccaatttg aagaggatag taagcttata
     3181 attgaagata tatatgattg tatatttaat agtataagtg agattgatag tgttgaaatt
     3241 ttatataact ataatcttga aaaaataaac tttacatcaa gtgaaatcat aaattgtata
     3301 tgtagagaat atgagtgtta tggtgaatac ttagaagaaa aagaaaagct tatgaataca
     3361 tttaaagaaa agattattca aatctttgag taataggtca atgctgaagt ggcgctaatg
     3421 cgccattttt caaatatata tgaattaagt aaaattattt tggaggaggc gatttgtata
     3481 gagaaaacag gtgtaatttt ggcaggggga aaaaattcta gaatgggtag agataaagcc
     3541 tttttagagt tacatgataa gctttttatt gaaattgcaa tagaagcatt taaaaacttt
     3601 gatgagttga taataatatc taacaatgaa gaattatatt ctaaatacga tattaaagtt
     3661 tataatgata tagtaaaaga tgttggtcca attggaggaa tatatactgc tcttgaatat
     3721 gcaaagtatg atatagttac aattgcatgt gatatgccat atctaaataa tcaaatagtc
     3781 gaaagaatag ctaataaaat ggatgataaa agtgtgattt cagtgacaaa tggaaagtta
     3841 caaccattgt gttctggata taaaaagagt ataataaata aagtatcttt atgtataagg
     3901 gaaaatgatt taaagctcag aagctttata gataaaatag ataaaagtta tatatacttt
     3961 gatgaagaag atttattttt aaatgtaaac actgttgatg agtataaaaa attaactaaa
     4021 gtttaattag ctatggttgt aaaatagagt aggatttata tggaggagag tttatgaatt
     4081 tacttaaatg cgattcttgg gcagtaatat taaataatag cgataaagaa agtaaagcat
     4141 ataaaatatt agatgaactt aaaaggaata tgtacaaagt tgttgctata gatgaagaga
     4201 aaaaacctat tgaaggaata gatgtgtatg aatgtttaaa agatgttcca cacaatattg
     4261 atgttgtagc tattattgat aaacaatcta agatggatgt gattttagaa gaagtagagc
     4321 ttttggatat acaaaatatg tggtttgaaa aaggaagctt tagtgaaatg atgattaaaa
     4381 agactaaaga tttaaaatta aatatagaat ataatttaag tttatatgat gagttgacta
     4441 gataaaatta tatttcttaa taataaaaat ataattagtt aataaaacat attattttat
     4501 agtatagatt ggttaataaa agtacagatt ggttaatgaa acattaaatt acaatatttg
     4561 ttaaaaaaat tgtgagttac atgatataat gtattaaata ggatactaag gagttgatat
     4621 attatgttaa tagtaactac agaaaaagta gaaggtaaaa agatatcaaa ggttttagga
     4681 ttagtgagag gaagtacaat aagagcaaaa catgttggaa aagatatagg agcaagtttt
     4741 aagaatcttg ttggaggaga acttactgga tataatgaaa tgctcactga agcaagacaa
     4801 attgccatag gtagaatggt tgaagatgca gaagctaaag gtgcaaacgc agtaatagca
     4861 tttagactgt cttcagcttc agttatgcaa ggagcagcag aaatgcttgc ttatggaaca
     4921 gcagttgttt tagaagatga taatagtatt cttgaaaaat aaaatataaa tagttaaaaa
     4981 gaggcatctt ttaaaaattt tatggatgcc tttttttatt tgaaaaaact aatttgagaa
     5041 gaaattttaa ctattatgac aataaaatta gggtgatatt atataaataa gtaaaattat
     5101 attatataat gtatggtata gactttaaaa attttatttg tagagaaaaa tatggacaat
     5161 gttataataa ttatagaaat taaaagtact tacattcatt atgtatgtga catgtataat
     5221 tagttttata tttaatacag ttttgaagta atatcttcat tttgagaacg ttggaataat
     5281 aaaaaatgta tgtattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     5341 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     5401 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     5461 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     5521 aaatacagga gcttataaag ttactgaata tgtacttata aaagatgata aggaaatgct
     5581 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     5641 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     5701 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     5761 catactaagt aggggtgtga gtggaataag aaataattct ataataataa atttacctgg
     5821 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     5881 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     5941 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     6001 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtctattgta
     6061 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     6121 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     6181 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     6241 gaataaataa cataggaatg accactaatg ctattgggct ttctgataga atttttgagc
     6301 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     6361 ataaatccgt aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     6421 taaatttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     6481 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     6541 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     6601 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     6661 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     6721 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     6781 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     6841 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     6901 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     6961 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     7021 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     7081 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     7141 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     7201 atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     7261 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     7321 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg gagaagactg
     7381 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     7441 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg taattaaagt
     7501 tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     7561 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     7621 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     7681 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     7741 tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag gtgactgtgt
     7801 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     7861 ttcaatagat gtaatctaat gaagtcacta ttaaaatata attataagta aatactatta
     7921 agatttaaat atatatttta agagagaatt aattttaata aaatatttta gttataaatt
     7981 ggaat
//
LOCUS       DAELJO010000168         7953 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.170, whole genome shotgun sequence.
ACCESSION   DAELJO010000168 DAELJO010000000
VERSION     DAELJO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.170"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(420..1082)
                     /locus_tag="KPB88_003063"
                     /pseudo
     CDS             complement(420..1082)
                     /locus_tag="KPB88_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1242..1778)
                     /locus_tag="KPB88_003064"
     CDS             complement(1242..1778)
                     /locus_tag="KPB88_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0055322.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1851..2264)
                     /locus_tag="KPB88_003065"
     CDS             complement(1851..2264)
                     /locus_tag="KPB88_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0055323.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2730..3326
                     /locus_tag="KPB88_003066"
     CDS             2730..3326
                     /locus_tag="KPB88_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HBG0055324.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISGIDDTISDMQGSISSMNGTISDMQGSISSMNGTISDMQDSITD
                     MQVEIKEVNSRLDNMEPDIKDLKTGQIETSSRLDTIQSDVTEIKSDVKINRNTLDTVI
                     KHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3976..4113)
                     /locus_tag="KPB88_003067"
     CDS             complement(3976..4113)
                     /locus_tag="KPB88_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0055325.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     H"
     gene            complement(4338..4871)
                     /locus_tag="KPB88_003068"
     CDS             complement(4338..4871)
                     /locus_tag="KPB88_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0055326.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5188..5538
                     /locus_tag="KPB88_003069"
                     /pseudo
     CDS             5188..5538
                     /locus_tag="KPB88_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6619..7191)
                     /locus_tag="KPB88_003070"
     CDS             complement(6619..7191)
                     /locus_tag="KPB88_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0055327.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7253..7516)
                     /locus_tag="KPB88_003071"
     CDS             complement(7253..7516)
                     /locus_tag="KPB88_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0055328.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7516..7737)
                     /locus_tag="KPB88_003072"
     CDS             complement(7516..7737)
                     /locus_tag="KPB88_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0055329.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2741 a         1376 c          952 g         2884 t
ORIGIN      
        1 acctatgcag ttctcttatg aacttcttac actttcatgt aagcacagac tatatcttat
       61 ccttcggcat tatccgttaa ggtctaccca cttccactac cgataacttg tagtgtactt
      121 ccctcaagag gaatagtcgt tgaagtttct cctattcgga gcttacctgc tgattgccca
      181 ttttaaagta cttaggattt aaccttatac catctaacca attttttcta ctttcgtaac
      241 tttcacactt aggtttattt catccttatg ttttagtttg gttagcttta ggggtttcca
      301 gcaattcaag tagtattgga taggttctat cctatctcta catataggtt tccctatatg
      361 ctgactattt tagctactaa ctcatgacta aagtcacaag tgtgcgtagc tattttttaa
      421 tcaaactgga atacagactc cattcctagg aaattggctg aaatatagtc tgctgtcaaa
      481 gatgctgtca tcacagaaat taaaacagat actgaaaagt ttttatggac ttcttcaagt
      541 gaaaacataa cacctgctag tggtgcatga aaggctgctg ataatccagc acttgcccca
      601 caggttaaca ggaatttttc ctctgtcttc cctcggtcaa gagctctaga aataccttta
      661 cctgccatag caccaagctg aatggaagga ccttctcttc ccaaagcaag accacccatg
      721 agtgataaga atcctccaat aaattttgtt ggcaacactc gccaccatac ttgttttatt
      781 ttccctgtca tttccccctc aagctgaggg ataccacttc cagaaatcat tggctcccag
      841 tccaccaact ttgatacaat ccacgcaagt actgcaagta ctgcaaacca agctattatt
      901 tttattgtag aaccttctac aaattttaaa atttggctaa gccaagtacc agcctgccct
      961 aaaagtatcc tgtataaaag cacaatcaaa ccagccacag ccccaaccat aagtccttca
     1021 ccaatcaaaa ttacctgaaa gtgctctgcc cgtttgataa tatgagatgt atcctttttc
     1081 atactgttcc tccttcatcc aataattcta cacaatctga atctccaatg actaaagtca
     1141 tgagtgtgta cagatatttt ttaatcaaca aaaatagatt tttacttact aaatataaga
     1201 aaataattgc tcaatttctc aataaatgtg aatttattct tttaggtatt tattctccac
     1261 agtcgtgctt cttccagcaa aatatttatt agttcttgct ttctctgtgt atagatttta
     1321 cgattgtcct caaactgtaa tgccaatttt tgtttatgac tatcatagag ttttgctttg
     1381 tctgtaaaag tatttagata atctcgaaaa ttgatataat tattccatgc aacactattc
     1441 cattcaacaa catgaatatg gtgagtacga gtatttttct caaaatctcc catagcaaaa
     1501 agtaattgtt ttggtacatc ttgacctcga aaaacgattc cacattgctc taacctttcg
     1561 aggtataatt tagcttcctc taaagaagca acacctacaa caatatcaat aataggcttt
     1621 gcgctaatac tgggaataga agtgcttcca atgtgttgaa tatcaatggc agtcttatcc
     1681 catatacttt tcaataatat aatgacatct tttgcacttt tatcccatat catctgatgt
     1741 tccaataatt taaccatacc acattttaaa cctatcacta tataccacct caatataatc
     1801 cacctttata tatattacaa ttttttctct cttgataaac agtgatttag ctatttcact
     1861 ttttcttgac actcgctaca ttccctatca tgaatagata taattcctcc acatttagaa
     1921 caagtatatc tctccttctg cttttgcata aacttctcta aaccgtactg ctgaacaaat
     1981 ttactatttt ccataaggct tgcttgatat ctcttgttat aacttttttc aagatttttt
     2041 atgagtttac aaggaaaatt gaaacattca aaacaataaa acaacccttt ttgacagaca
     2101 caatccttta ttttacactt acgacagtgt tctggctttc ctgtatcgcc gttcaaacaa
     2161 ccagcacatg gctttttgtg ataacaatgc ttatagcaaa ccatacaatt cattccacaa
     2221 ggagcaaaca taatcgtatc aatactctct tttggcattt tcataacatt ttactccttt
     2281 caaaaatcca atttgtcgaa ttgttatata ttcatattgt aacataaaaa atagctaaaa
     2341 aattcatgca ctttcattgc caataaattt tcaaggaaat atgaaggttt aaaactaaga
     2401 ataaaaagag ataaaaagga agctactaat aaaaatattc aaatacaagt aatcaatata
     2461 aataacttaa ggtatattta aaagtatatg atattaccag cagttttaaa tacaaatata
     2521 accacacatg cctcaaaaac acttattaca gtatcttaga aacttagaca atacataaga
     2581 ttcattaata aaaaagttct caatatagat atatttaacc atgttttgaa tagcagataa
     2641 ttatatgatt aaaaattaaa tatgacatag cgtttgtgaa atataacacc taaattatgt
     2701 tataataata aaacaggagt gtgatagtga tggatgaaaa aatattagag ttattacagg
     2761 aaatgcaagg taatattagt aatatgcaag gtagtatcag tggtatcgaa ggcactatta
     2821 gtgatatgca aagcaatatt aatggtatcg aagacactat tagtggcatg caaggcacta
     2881 ttagcgatat gcaaggcagt atcagtggta tagatgacac tattagcgat atgcaaggca
     2941 gtatcagtag tatgaatggc actattagcg atatgcaagg cagtatcagt agtatgaatg
     3001 gcactattag cgatatgcaa gacagtatta ctgatatgca agttgaaatt aaagaagtca
     3061 atagtaggtt agacaatatg gaacctgata ttaaagactt aaaaactgga caaattgaaa
     3121 ctagtagtag acttgatact attcagagtg atgttacaga aattaagtca gatgtaaaaa
     3181 ttaacagaaa tacattagat acagtgatta aacatacctc ttcattagta aaagatacat
     3241 cagatattaa aaatgattta aataatatag agatagtcac atctaagaat tcgaatgata
     3301 ttgctaaatt aaaatcagtt aaataatata ccatagtaaa aaatacctga aaatttcaca
     3361 tacttcttta aagttatacc aaattttata cagaaatact gattatccga caacacactt
     3421 tacttataaa taatttatta aaaaaatact cattttatcc taaaattaac acgactaaat
     3481 gagtcttgat ataataataa aaaataaggg agattgaaca ctaaaaagtt gtgtatctcc
     3541 cttatattct ataatatagt tatataattt ttcagcctaa aataaaagtc catctttgat
     3601 taaaacacat ccccttatca cttataaaag gataaaatct tgttttcatt tttccattta
     3661 tatcttcttg cttaagagta ataatttttt cttttgatac tttaagtgtt tggttaaatt
     3721 taaccaatcg actaagtttt gttcaacatc tatttttcat attttccaat acttaatcta
     3781 ttttttataa ataattcttt catttctaac tcacttacat tttttacata aaacgaaaca
     3841 tataaatttc aaccttatct tgtatggttc ttaatactta atgagtagct taagtatcaa
     3901 tcattataaa tttttccctt acatttaaca ctcttaccat ttccaaaata tacaaaatat
     3961 attttgaaca cttgttcaat gatgaaatac aatacatgaa aatacaatat cataactctt
     4021 gtctaaaaat agaagttctt caacataacc tttcacaaaa aacatattat ctaagtattt
     4081 tttgtctgct ccttgttttc ctgtttgtag catataaatt actgcatcta aacatataac
     4141 tactcacacg aaaaaaaaaa taaacttcca aaatacaagc tctaaaaact acctctaaag
     4201 ttgtataatg tttactagga ataaggttgt ataacatata gtttaaataa tcatttttta
     4261 aaatcgcaag ttattgcttt caaaattaga tgtttgtata tcaaataaat gattaagaat
     4321 ttctaaattt aatggcttta taatgcactc cctttctttg ggataaaata atgttgcaca
     4381 gatacttttt ctaaagataa caattttatt tttctatcaa ttccaccagc atacccagtc
     4441 aaactcccat ttgttcctac aacacgatga catggaataa taatagagat tccattatgc
     4501 cctactgcac caccgattgc ttgtgctgac attttagata ttcctttttg ttgagcaact
     4561 ttatatgcaa tttctccata agtgattgtt ttaccatatg gtatttgttg taaaattttc
     4621 cacactgtag tctgaaaagg agtacctacc atatggagtg atggtgtaaa attgggttct
     4681 tttcccgaaa aataaatatc taaccatttc tttgtctgct cgaaaattgg caattctttt
     4741 tcttgatgtt cttggtcaag attataagca taatactttt gattctcaaa ccaaagacca
     4801 gttaacccaa tattatctga tgctaaaaga atatttccaa ttggactatg gtaatagcta
     4861 atatattgca tacaattcct ccttatattc tgaaaacttc actcaaatca ttagacactt
     4921 cttttctatc taccattatg taaaaacata ctaatataat ttccttctaa ctacactttc
     4981 gttataatat ctatcctcat atcatattag ttttatattt acctgcatct gttcatcctt
     5041 ttttcccttt ccctttgatg gatataaaat tcatacattt aagcttccta ctgtattaac
     5101 ttttatagaa gtgtatctta taattttatc actaaatagg ccaaaaaaat atcgatatag
     5161 catcaagtaa tatcataata ctttcaaatg ggaaaagtaa gaataggctg taaaaaagaa
     5221 aagtattttt atgttagaaa tccagtaaaa tcattagtag atagaataac agaaaacgaa
     5281 caatttgcta tttatcattt tacacataaa tataattgac gcaataaagt gttttctatt
     5341 ctacaacgat taataaggtt agaaaaaagt aaaaatgaac tgtatatgta tgaagcatta
     5401 gtttctgttt ctttcctttg gcttattatg ataaaaaata ttgttttgcc accaataaaa
     5461 cacaaaaata taattaatat ccatatgcaa aaaattttac gttatattga aaaatactat
     5521 tgttttacat tatattgaag ggtactattg tgaaaactta attttggagt actatcaagc
     5581 agtggtaata tcaataaatc agagtgctcc agatgatttg aactaagttt aaatactata
     5641 ccttataaat attttactga gttttgttta ttaaaagcaa ctcaatttct aaaaggcaca
     5701 aatgagctaa taagtgacat tgctataaaa gttgtttttc atcaaataat ctattttagt
     5761 aaatgtttta aagaaaaaac gggatattct ccaaaagtgt acagaaatat aaaaaaagag
     5821 atatgatttt ttatttcttt tgattatcca aagagtaaat aaaaaatatt gtaaaaaact
     5881 tactaacaac ggcaatgctt tgaactatgg taatactttg aacttttgat tggacttttt
     5941 ttgctaaaat aatatattta aatagcaaag taatctgttt aatcaataaa aaataagaga
     6001 taaaagaaat caactaaaac ttttacctct tattttgata gtttatcaga gctaatattt
     6061 acactctttt tatttttgat acttcattaa aaatgatgct tactctccat tcacttttaa
     6121 ttcttcatta aaaatgatgc ttattctcca ttcactttta attcttcatt aaaaatgatg
     6181 cttactctcc attcgctttt aattcttcat taaaaatgat gcttattctc cattcacttt
     6241 taattcttca ttaaaaatga tgcttactct ccattcgctt ttaattcttc attagaacta
     6301 tgcttactct ccattcactt ttaattcttc attaaaaatg atgcttactc tccattcaat
     6361 tttgatactt cattaaaaat gatgcttatt ctcttttcac ttttaattct tcattagaaa
     6421 tgatgcttat tctccattca cttttaattc ttcattaaaa atgatgctta ctctctgttc
     6481 acttttaatt cttcattaaa aatgatgctt attctccatt cacttttgat tcttcattaa
     6541 aaatgatgct tattctctgt tcacttttaa ttctttatta aaactgatgc ttattctcca
     6601 ttcactttca attcttcatt aaaactgatg cttgcactct gtaaactcat aaacatccat
     6661 taatgcttca ccaaatcttt gatagtgaac tacttccctc tcacccaaaa atctcaatat
     6721 atctttcaaa tcagggtcat cagttaaatt tattaattga tagtacgtag ccaatgcttt
     6781 ttgttcagca gccatatcct catgtaaatc agttaccgaa tcgccaaaga cattaatata
     6841 acttgtagta aagtttactc cattagaatc tactggctga agaccatgtc caaaattagc
     6901 ataattacta ccaagtccag cagccttaag ttcttctgga gtacaattgc ctattaattg
     6961 atataacact gaagaaatta tctcaacatg agccatttcc tctgtaccta tatcagttaa
     7021 aagtgcacga gattttccag ttggcatagt atatctttgt tgaagatatc tagttgcagc
     7081 tgccaactca ccattaggtc ctcccaactg agtcatgata tattttgcca ttctagggtc
     7141 tgcttgtctt atgttaactg gatgttccag tgttttttga taaatccaca tattatctta
     7201 ctcctcctta atatatattt aataataaat ctaattttaa agtttaataa ttttatttat
     7261 aaaatgattt gtcccatggc caaggttcag atatccatga ccaaggatat tcacttgatt
     7321 catatccaaa attagttaat gctccatatt tagattcata atcacacaca gcttgtgtaa
     7381 atttatcgca gtatctgttg tatgaatcaa gagctttctt atcccttgga ttgttatcta
     7441 aatataaatt taattctata caggcaaatt gatattctga aattctctct aaaagttctc
     7501 ttctatttaa tgacattagt acatgttacc ccctttttta gctctaactc tatttttagg
     7561 atttgcatat agccaatcat tatagttatt tgactctatt aaatctaatt caggaaataa
     7621 agtacctctt tttaatgctg tttttggatt gtatgtgtca tcataaattt gacgattgat
     7681 gtatggtcta gcaaattcac aacaatagtc ttgtttatcc tcttttaaat atcccattaa
     7741 ttatcctcct taatttttag aattttaaaa tagcattatt tttaaaaatt gtatactggt
     7801 tctatattat aactggtagt tttaataggt ctttaataag attaaatgat taaataggta
     7861 tctttttttc ttattttacc tctaaaaatt tttctaatta gtctttcata aatatctatt
     7921 ttaaataaat aatatagagt ttatacaatt ttt
//
LOCUS       DAELJO010000169         7938 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELJO010000169 DAELJO010000000
VERSION     DAELJO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.171"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            27..101
                     /locus_tag="KPB88_003073"
     tRNA            27..101
                     /locus_tag="KPB88_003073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPB88_003074"
     tRNA            116..200
                     /locus_tag="KPB88_003074"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPB88_003075"
     tRNA            210..293
                     /locus_tag="KPB88_003075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPB88_003076"
     tRNA            317..391
                     /locus_tag="KPB88_003076"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPB88_003077"
     tRNA            416..492
                     /locus_tag="KPB88_003077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            500..575
                     /locus_tag="KPB88_003078"
     tRNA            500..575
                     /locus_tag="KPB88_003078"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Gln,seq:ttg)
     gene            584..659
                     /locus_tag="KPB88_003079"
     tRNA            584..659
                     /locus_tag="KPB88_003079"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Lys,seq:ttt)
     gene            662..750
                     /locus_tag="KPB88_003080"
     tRNA            662..750
                     /locus_tag="KPB88_003080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Ser,seq:tga)
     gene            754..829
                     /locus_tag="KPB88_003081"
     tRNA            754..829
                     /locus_tag="KPB88_003081"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Phe,seq:gaa)
     gene            836..909
                     /locus_tag="KPB88_003082"
     tRNA            836..909
                     /locus_tag="KPB88_003082"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Met,seq:cat)
     gene            924..1000
                     /locus_tag="KPB88_003083"
     tRNA            924..1000
                     /locus_tag="KPB88_003083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:958..960,aa:Ile,seq:cat)
     gene            1018..1094
                     /locus_tag="KPB88_003084"
     tRNA            1018..1094
                     /locus_tag="KPB88_003084"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1052..1054,aa:His,seq:gtg)
     gene            1103..1178
                     /locus_tag="KPB88_003085"
     tRNA            1103..1178
                     /locus_tag="KPB88_003085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Lys,seq:ttt)
     gene            1186..1259
                     /locus_tag="KPB88_003086"
     tRNA            1186..1259
                     /locus_tag="KPB88_003086"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1218..1220,aa:Cys,seq:gca)
     gene            1267..1343
                     /locus_tag="KPB88_003087"
     tRNA            1267..1343
                     /locus_tag="KPB88_003087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Arg,seq:acg)
     gene            1356..1431
                     /locus_tag="KPB88_003088"
     tRNA            1356..1431
                     /locus_tag="KPB88_003088"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:Val,seq:tac)
     gene            1507..1920
                     /locus_tag="KPB88_003089"
     CDS             1507..1920
                     /locus_tag="KPB88_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055330.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2229..5660
                     /locus_tag="KPB88_003090"
     CDS             2229..5660
                     /locus_tag="KPB88_003090"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG0055331.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5902..7830
                     /locus_tag="KPB88_003091"
     CDS             5902..7830
                     /locus_tag="KPB88_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0055332.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3109 a         1016 c         1590 g         2223 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatatt ggggattagc caagtcggta aggcacatga ctttgactca
      541 tgtatgcgta ggttcgagtc ctgcatcccc agccaaacag tatggtctat tagctcagtc
      601 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
      661 tggagaggtg tccgagtggt ttaaggagct ggtcttgaaa accagtgact tcgaaagggg
      721 ccgtgggttc gaatcccacc ctctccgcca aatgcccaga tagctcagtc ggtagagcag
      781 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccaa aatgtggcgg
      841 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      901 ttaccgctac taaaatttaa tgtgggccat tagctcagtt ggttagagcg cccggctcat
      961 aaccggtagg tctggggttc gagtccctga tggcccacca cattaaatta acttatggtg
     1021 ggtatagctc agttggttag agcgccagat tgtggctctg gaggtcgtga gttcgactct
     1081 cattatccac cccaacttat atggtctatt agctcagtcg gtagagcacc tgacttttaa
     1141 tcagggtgtc ccgcgttcga gtcgcggata gatcaccaaa aaggcggcga catagccaag
     1201 tggtaaggca gtggactgca actccttgat ccccagttcg aatctgggtg tcgcctccaa
     1261 taaaatgcgc tcatagctca actggataga gtgtctgact acgaatcaga aggttagggg
     1321 ttcgagtccc tttgggcgca ccatatgaat agcgagggat tatagctcag ctgggagagc
     1381 acctgcctta caagcagggg gtcacaggtt cgagccctgt taatcccacc agtgcaaacc
     1441 tctagtaaaa ctactagagg ttttattttt gattgaaaaa atttttatat ttaggaggta
     1501 aaaaatttgt atatagtaat aggaaatgaa atagttgata gtgaagaact taaaataatt
     1561 atagataaaa atagtgaatt taaagttgaa aaagatttaa gtaaaagcac aaaaagagaa
     1621 gatgtaatag catatcagtt aagtatagat ttaaattacc tagattctct tataaatgag
     1681 caatgtaatt tagccagtct atctgatgaa gagaaatttg atgaatatat gactttgtct
     1741 gatgaacttg cactggattt agaagagtta atgccaaaat atacaataat aaatgctaga
     1801 gcatataaat tagacgaggt atatggaatt gtaaaaataa tactagctgt agcgtatgca
     1861 gatttagggc acttaaaact ttctgatgta gtaaagaggc tatctagaca agtagattag
     1921 gatatgaata tgaactatat taaaaaaatg tataaaatct tacgtaaaga tgaaaataat
     1981 tatgtaaaca tagaaaaaaa cagtataaaa tcaagtcaat tgtagaaatt ttcacataaa
     2041 ttcatctgaa attctataaa aaatgcctta attttaatga ggtaaatcga gtttacacag
     2101 tctaaaaatt acttatattt ataaatttat cttattaatt aaggaacttt cttaaacatc
     2161 ttcaaaaata aagtatttta gtctataatc tttaatatta cacaaacaat aaaggagaag
     2221 aatagagtat gcttaaaaag tttaataaaa tactagtggc taatagaggc gaaatagcta
     2281 taagaatatt tagagcctgt tcagaattgg ggataaaaag tgttggtata tacagtaagg
     2341 aagataagta tggattgttt agaacaaaag cggatgaatc atatttgata ggagaaggta
     2401 aagggccaat agatgcatat ttagatatgg atggaataat agacctagca aagagaaaaa
     2461 aagttgatgc tatacatcca ggatatggtt ttctagcgga gaatgcagaa tttgctcgta
     2521 aatgtgaaga gaatggaatt acttttatag gaccatcatc taaggtaatg aatatgatgg
     2581 gggataagat taactctaaa aaaatcgcaa aagaagttaa tgttcaaact ataccaggag
     2641 tagaaaaagc aataagaagt actgaagaag caaaggaagt tgcaaacaaa ataggatatc
     2701 ctgttatgat aaaagcatct aatggcggtg gcggtagagg tatgagaatt gtacatcgcg
     2761 aagaagactt agagcttgaa tatgaaactg cttgtagtga atcaagaaaa gcttttggag
     2821 aagatattat atttatagaa aaatatatag ctgatccaaa acatatagaa gttcaaatat
     2881 taggtgataa ttatggaaat atagttcact tatatgaaag agattgttca gttcaaagaa
     2941 gacatcaaaa aataatagaa tatgcaccag ctttttcgtt agatgataaa gtaagaaaag
     3001 agatatgtga agatgcagtt aagttatcaa aacatgtggg ttattctaat gcgggaacat
     3061 tagaattttt agtagatgca aatggtgggc attattttat agagatgaac acaagagtac
     3121 aggttgaaca tactgtaaca gagatggtta caggaataga tatagttcaa agtcaaatac
     3181 taatagccca aggttacagc ttagatagtg aagaaattaa tataaaatca caagatgatg
     3241 tggaaataag aggttactct atccaatgta gaataactac agaagaccct aagaataaat
     3301 tcatgcctga tacaggtaaa attcaagttt atagaactgg ttcaggattt ggtataagac
     3361 tagatggagg aaatggattt acaggggcaa acataagtcc tcattatgat agtttattgg
     3421 ttaagacaat atcttgggat agaacattcc aaggagcaat aaataaaact ataagatcta
     3481 taaaagaact tagagttaga ggtgttaaaa ctaatgtagg attcctagtt aatgttttaa
     3541 ataatcctat attctcaaat ggtaaatgta gtactaagtt tatagatgaa aatccagatt
     3601 tatttgaaat aacagaaagt aaagacagag gaacaaaatt acttcaattt ataggagatg
     3661 taattgtaaa tgataatgct tgtaaagaaa aaccattatt tgatgcatta catgacccaa
     3721 gaatggataa ggatggttca aaatcagaag gaagtaaaat acttttcgat aagttaggta
     3781 aatctgcata tatagaaaag ataaaaaatg ataagaaatt actattaaca gatacaacaa
     3841 tgagagatgc acatcaatca ttattagcaa caagaataag aacatatgac ttattaaaag
     3901 ctgctaagcc aacagaaaaa tatcaaaagg atttattctc acttgaaatg tggggaggag
     3961 ctacttatga tgttgcatat agatttttaa aagaatctcc ttggcgtaga ttacaaaagc
     4021 taagagaaga aataccaagc ataatgttcc aaatgttgct tagagcatca aatggagttg
     4081 gatataaaaa ctatccagat aatgtaatag aagaatttac aaaagaatct gctagacaag
     4141 gtatagatgt atttagaata tttgattcat taaactgggt tgaaaatatg aaaccatcta
     4201 ttaatacagc tttagaaact ggaaaaattg ttgaagcaac tatgtgttat acaggtgata
     4261 tactagataa gactaaaact aaatacaatc tagaatatta tattaaaatg gcacaagaat
     4321 tagaaagctt gggagcagat ataatagcaa taaaagatat gtcaggtctt cttaaaccat
     4381 attcagctta cacactagtt aaagagttaa agaaaaatgt aaaagcacca atacatttac
     4441 acactcatga tacaagtgga aatggtgtag caacttgttt aatggcgtca gaagctggag
     4501 tagacataat tgatgctgca ttagaatcta tggcaggact tacaagtcag ccatcactta
     4561 atgctatagt agaggcactt aagaatacag agagagatac aggaatagat ttatttggat
     4621 atgatgagtt aggaaaatat tataaggatt taagaaaagt ttataataaa tttgaaagtg
     4681 atttaactaa ttcttgtgca gaaatatata attttgaaat accaggaggc caatatacaa
     4741 acttaaaacc tcaagctgat agtttaggat tagttaatag atttgatgaa gttaaagaaa
     4801 aatataaaga agctaatgaa gttgtaggag atataataaa agtaactcca tcttcaaaag
     4861 ttgtaggaga cttagctatc tttatgacta aaaataaatt agataaagat aatataatag
     4921 aagaaggtaa aaatttatca ttcccagatt ctgttgtgga ttactgtaaa ggtatgattg
     4981 gtcaaccaga aggtggaata ccaaaagatt tacaagaggt agttctaaaa ggtgaagaag
     5041 ctataacagt aagaccaggt tcattattac cagcagaaga ttttgatgaa atagctaaat
     5101 atctaaatga aaagtatgat ataaatgcta atataagaaa tgttataagt tatgctttat
     5161 atccaaaagt atatgaagat tatatcaaac atctacaaca ttataatgat atatctaaac
     5221 ttgaaagtga tgtattcttc tatggattaa ataaaaatga agaatgtgaa gtagaaatag
     5281 aagaaggtaa ggtattaaca ataagattag ttgaaattgg tgaagttaag gaaaatggat
     5341 ttagaactat aggatttgag ttaaatggaa tggttagaga agtagaaata aaagataaga
     5401 atttctctgg aaaaattaat aatgttgaaa aagctgatat gaatgaccct cttcaaattg
     5461 gagcaagcat accaggaaaa gttataaaaa taatggttaa agaagaagat gaagtaaaag
     5521 caaatcaacc attaatcgtt atagaggcta tgaagatgga aactataata gttgctaaga
     5581 ctgatggagt aattaagtca atcaaagtaa aagaagatga tatggttgaa gataaacaat
     5641 tattaatgat aatgaagtaa tttaattaaa ataaattaaa aagcactctg aaatgtttaa
     5701 taaaaaatgt tttagagtgt ttttatttat tttaattaaa tatttgttaa aaaaaagagg
     5761 acaattacct ccagatgtag aaataaagct agtaaggaaa atgttatcta aaaattctaa
     5821 atatagtaaa tattttccat aatatttctt aggaaataac agcaaagata aaatacataa
     5881 atttaaaagg tgggtgctta tatgaaggtt tatgatagta aaatgttgag aaatgtagcg
     5941 gtattaggac atagcggatg tggaaaaaca aatctaatcg aaactatagc ttacacagca
     6001 aacactaata agatacctaa attaacagac aaggtaaata tgacttatag catgggatta
     6061 atacctatag agtataatga ttataagttt aacttattgg ataccccagg atattttgac
     6121 tttagcggag acgttgtgtc atctcttaga gcaagtgatg cagcaataat agtaatagat
     6181 gcaacagcgc ctattcaagt tggtacagag aaatcattag aactaactga gagtattcct
     6241 aagataatgt ttataaataa aatagataat gaaaaggcaa gatacaaaga tgcaatagca
     6301 atgttaagag aaaaatacaa taataagata gttcctatga taagtcctat atacaaagat
     6361 aaaaactttg taaagttaca taatgttttt gaaaacatag atgatttaga aggtgaattt
     6421 aaggaacaag ctatgagtgt taaagaagca ttaatggaac ttatagcaga aactgatgac
     6481 caaatattag ataaatattt taatggagaa gaactaacta cagaagagat acaaaaaggt
     6541 attattatag gtatacaaag aggtgatatt atacctgtaa tatgtggttc aactataaat
     6601 aatataggca caaaggaaat attagataca atatcaagtt atttagaacc tatctttaca
     6661 gaggaaagca aaccatttag gggattagta tttaaaacta tggttgaccc atttgttgga
     6721 aagatgtcat atataaaaat tactgagggt gtactttcta aggataaaga tgtttttaat
     6781 ataaataaaa atgtaaaaga aaaaatagct aatatatata cattgagaaa tagtgaatta
     6841 gtagaaatag aaaaagctaa agctggtgat atagtagtta taactaaagt taattctcta
     6901 aaaacaggag acactatatc tgcagataaa gatgcagaag ctttagaaaa gatagatttt
     6961 cctaaaccac aaatatatta tgcagtaact cctaagaata aaggtgatga agaaaaagta
     7021 gcatctgtat taaataaact agtagaagag gaccctacat tacattggta tagaaataca
     7081 gagacaaaac aagcactttt aggaggtcaa ggagaacttc atataaaaac aattaaaaat
     7141 aaaatgaaag ataagtttgg tgttgatgtt gaattgaatg accttaaagt tccttataga
     7201 gaaactataa aaggaacagc tgatgtacaa ggaaaacata aaaagcaatc aggtggtcat
     7261 ggtcaatatg gagacgtaaa aataagattt gaaagatgtg aatctgattt tgaatttaca
     7321 gaagaaatat ttggtggttc tgtaccaaaa caatatatac cagcagttga aaaaggttta
     7381 aaagactcta tgcaaaaagg tatattagca ggatatcctg ttacaaacat aaaagctacg
     7441 ctttatgatg gttcatacca tgatgtagac tcttctgaaa tggcctttaa aatggcagca
     7501 agtgcagcct ttaaaaaagg tatggaagaa gcacacccaa tactactaga acctataatg
     7561 aaacttaaga taactgttcc agaagaatat atgggagatg tcatgggtga tataaataag
     7621 agaagaggaa aaatatttgg aatggaacca gatgacaaag ggaaacaaat aatatttgca
     7681 gaagctccac aagcagagac atttaaatat gcaatagatt taagagcaat gacacaaggt
     7741 agaggatact ttgaaatgga attagagaga tatggagagg ttccatcaca gtttgcagaa
     7801 aaaataattg ggctagctac tgcaaagtaa tgaacaaaat aaatagaaaa ataattaata
     7861 tatattatta aaaatatttt tagggtttta actaggtata ggtttatgtt aggtaaactt
     7921 atacctagtt tttataca
//
LOCUS       DAELJO010000170         7833 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELJO010000170 DAELJO010000000
VERSION     DAELJO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.172"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(335..2614)
                     /locus_tag="KPB88_003092"
     CDS             complement(335..2614)
                     /locus_tag="KPB88_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0055333.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2885..4339)
                     /locus_tag="KPB88_003093"
     CDS             complement(2885..4339)
                     /locus_tag="KPB88_003093"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG0055334.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4503..5372
                     /locus_tag="KPB88_003094"
     CDS             4503..5372
                     /locus_tag="KPB88_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055335.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDIFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5585..7657)
                     /locus_tag="KPB88_003095"
     CDS             complement(5585..7657)
                     /locus_tag="KPB88_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0055336.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PVLANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2665 a         1297 c          827 g         3044 t
ORIGIN      
        1 aaaactcttt ttttgatata ataaaattac ttacatatat aacatttaaa tttataaatt
       61 atctttttat tttttaaaat atgtataaca cacctataga aattactatt attactatct
      121 aaagtatttt gctataatcc catatactct tacctcaaaa acatccaaat gtaaaaaata
      181 ctacatatta ataaactttg cccaataatt cctaaaataa tatcctaaca atgatttatg
      241 tttttataat agtatatttt tacataaaaa agtgaacatt gattaacaaa gttcactttt
      301 ttataaattt ttatgttata attcacaatt ttatttaatt tgtttctttt ctatttaaac
      361 tatatctatc taatattaat ttttcatatt cttctattgg cattggtcta gcaaataaaa
      421 atccttgggc catatcacaa cctataaacc ttaaaaactc tgcttgctct acagtttcaa
      481 caccttctga aacaactttt atatcaagtt gctttgccat ttgaacaata cttgaaacta
      541 caatttgact cttttttgca tttcccactg ttataaagaa ttgcctatct aatttcaata
      601 catcaattgg catatcttta agcatattta aagaagaata accagaacca aaatcatcca
      661 tagaaatttt aaatccaaca gatttcaaag tttgcattat tttaaataat atttttgtat
      721 tgtcaaatat tgcactttct gtcaattcaa tctcaattaa atttggttca atttcatatt
      781 cttttgttat tctttcaagc tcagaaataa agcctggatt ctcaagatgt actcttgata
      841 agtttattga tataggaaat gtggggaaat tttcattttc ccaacgttta aaatttttac
      901 aaacttgggt aaatacaaac atatccaatt ctgttataaa gccatttttc tcaaaaatag
      961 gaataaatac acctggactt attagacctt ttttaggatg ttgccaacgt acaagtgctt
     1021 cagcacctac aattttagta gttgataaat caaccttagg ctgtaaataa actttaaatt
     1081 gttcattttt taaagcatca tgcatttctt gttcaatatc acgttcttct atgagagttt
     1141 cttttaaatt ttcagcataa atctcataaa cttttaaata ttttcttttt actgtagttt
     1201 ttgcaatcat agctttgtca agacaggtag aaatatttgt ttctccctct tcaacaaaat
     1261 atacacctat ggatggaatt aaattgagtt ctactcccaa acttgttttt atcattgaca
     1321 attttttacg tataagttca catatttcta taattgactc tctattattt ttcttttcaa
     1381 atattatagc aaaattgtca tttgaaattc ttgaaaatac agattgctct ttaaatatat
     1441 tcttaattat ctctgctatt tcctttaata ctctatctcc ttcttcatac ccaaacctat
     1501 catttactag cttaaactta tctatatcaa aataaattaa aactagattc tttttctcat
     1561 gagaggataa aaattttccc ccttcaagat taaatttatt tgaattgcca atacctgtaa
     1621 cgttatcttt aaaagcaaat tcttccaatt tttccttact ctttttatgt ataaatagta
     1681 tataaattgt taatgccgta aagattataa tcaagactac acatgctgat aatgtaagtt
     1741 ttataatagc atatgatttc tcagatatag cagttttagg aacttcagaa aaaagatacc
     1801 aatcattgat tcctattggg gcataactta aataatatcc ttttccaaac atactataac
     1861 ttaatgaacc tgatttaaag ttttttatat ttgacttcat attgttgata ctagattttg
     1921 atattgaatc acttctgcca atgtaagaaa ataaattttc tttagtagag ttggcgtttt
     1981 tactgcttgg atttaaaaca atatcaccat tacttctagt tatataagaa tatccttttc
     2041 cattaaatgt aggtacagaa agtatttttt catacaactt cgtactatgt gtggcaaata
     2101 taactccttt aacctcatta tctttataaa ttggtacaga atatacatta atattactat
     2161 tggtattttt atcatcttct attacatctt ttaatttgcc tgttataacc acttctcctt
     2221 gcattgattt taagaaataa tctcttttag agaagtcttc tatataacca tctgtagtgt
     2281 atgctgtacc atttggtaaa ataactccca ttcttttaaa tgaactatta atagcttgtc
     2341 tttttagtat agaaaggata ttatctactt caaaagactc ttctccttca ataaatattg
     2401 aaattgactc tagtaacttt atatctccat taatttgttt ttttaacaca ttgacacttt
     2461 gatttgctat ttctgaaaga tattgattcg tttcttcacc taatgtctta ttcaaccttg
     2521 aaccataaat caaacaggaa attgtaatta ctaagatact tatgacagca attatggaca
     2581 aaccatacct attatttttt gcatcactta acattatgct tcctgccttt ctcgaatcta
     2641 tgtatgtata atcattatct tatgacaatt attgtaaaca ttttataata atacttattt
     2701 ttccatattt actgtaatta tacaaaatta taaactttat tacaacaata aattaattaa
     2761 tatagatatt agtgtttaca ttattgcatt atctatttta caattattta catataaaaa
     2821 aatgtaatat caaagtgaag cttgatatta catttttgtt cttgtatttt gtttaatttt
     2881 tatgctacat cttacttaaa cactcatttg caacttcttt atcatcaaaa tgatgcttaa
     2941 ctttacctat aatttgataa gtttcatgtc ccttacctgt tataattaca acatcatcct
     3001 tttttgccat acttatagct tctgctatag cttgttctct atcaacaaca acatggtaat
     3061 tttctttttt cttgtcaatt ccttcaagta tatcttttat tattgcctct ggttcttcag
     3121 ttcttggatt atcagaagtt ataatacata aatcagaata tttttgtcca attgctccca
     3181 tcaaaggtct cttttcagta tctctatctc caccacaccc aaacacagaa attatttttc
     3241 cttctgcaaa ttgttgagtt gtctttagta cattttcaag tgcgtcagga gtatgagcat
     3301 agtctacaat tacgcttata cctttatcat ttggaactgt ttcaaacctt cctgatactc
     3361 cattagaaag tctaaggcct tctttatata ttggtttagg tatgccaagt acatagcaag
     3421 cagctataac tgctaaagta ttatatactg taaacattcc tggaactggt ataaatatta
     3481 cttcttcata tgaaggtgtt attaatctat aagatactcc atccgaatct gattttatat
     3541 ctctagccat aaaatcagcc ttagtatcaa caccataagt gtaacaaggt acatttatac
     3601 cttttatatt ttcatatatc ttttttccac cttcatcatc tatgtttatt atatttgcca
     3661 tagtagtctt aaaaaataat ttttcttttg cttttctata atcctctaaa tctttatgaa
     3721 aatctaaatg gtctggtgtt aaatttgtaa atatgcctag tttaaagtct gtttcatcaa
     3781 ctctatttaa tgccaatgaa tgtgatgaaa cttccatcgc acagtagtca catccattat
     3841 ccaacatatt attaaaatga tattgtaaat ctatactttc tggagttgtt gaattggaaa
     3901 caatgtcttt ttctccatca aaaattttta tagttcctat aagtccaact ttattcttat
     3961 ttaaagttaa tatctcattt agtatagtag ttatactagt ttttccatta gtacctgtaa
     4021 ctcctataac gttaaatttt tgagatggat gattgtaaaa attatcagct acttttgcca
     4081 tatcttttct tgtatctttt acctttataa atgtacatcc ttttattgct acatcttctt
     4141 caacaagaaa tgcacttgct cccttttcta tggcatcttt tatatactta tggccatctg
     4201 acacaaatcc ttttatacat ataaataatg taccttttgt aacctttctt gaatcatatt
     4261 gtacattatt aatatctata tttaattcac ccttgacatt gattatatcc aacccctgta
     4321 taatatcatt taatttcata aatgctttcc tccctttaaa ctctttctgc taaaattaca
     4381 ctacatataa tattatatta agaatttttt actatgtgaa tggttaaaaa tgcttgaaac
     4441 attgcaaaaa atgctctata attttctata atataactga ggaaaggtct tctataaaaa
     4501 ctatggataa taaaatcggg tacttaaacg ataactttaa aatatttaat attagagata
     4561 aaaaagatat acaatttgaa taccataatc atgattttaa taagataatt atatttataa
     4621 atggaaatgt aacttatttt gttgatggta ttccctataa actaaagcct tgggatatac
     4681 tctttatcag caataatgaa atccataaac cagttataga ttcaaatgtc ttttatgaga
     4741 gaattgcaat atggattagc ccagaattta tgaaaaaaaa tagtgatgct agtagtgact
     4801 tagaaaaatg ttttaaaata gctacagaga ataaatgcaa tagattgcgt ttaaatatac
     4861 atgatattga atctattaag agttttattg agcaaataaa tatttccgaa tccaatactg
     4921 aatttggaaa tgaaatattg agaaatacat tatttataca acttatgata ttcttaaaca
     4981 gaattttcct aaaaaatgaa aatatggaat caacagaaga tattttttat gatgaaacta
     5041 ttaagaaagt attaaactat ataaattcaa atatagatga taagctttca atagatagca
     5101 tatcgtctga attttttata agtaagtatt atcttatgag aaaatttaaa tctcatactg
     5161 gaacgtctat acacaattat atagttcaaa aaagattaat tttggcaaag tcactaataa
     5221 ccaatggtca ccttatgagt gaagtttgta ataaatgtgg attcaacgat tattctagtt
     5281 ttgtaagggc atttaaaaaa aactatgggg tatctcctaa aaattatata aacaataaaa
     5341 atcctttgga tattactctt ttagatgaat agataattta tgtaaatttt attttaataa
     5401 aacttgaagt attagtgtat ttatcttact aatacctcaa gttttttaat gttttaaaat
     5461 atatcaaaat ataatgatat atatttctat tacttaaaat atatattaaa taaattgcat
     5521 gttaattgaa cctatttata tatttattaa atcccacata acttttagtt acactatcat
     5581 atagctatcc tattttttca atatcatctc ttaatatact tactctattc tcctcttttg
     5641 ctgtggttac tcctttttta atgcttaatt tctgattttg ttctaaattc gtaaatacta
     5701 cagggcttat tgttgataga ccattttttt caatatattc taagtccatt ttaactaatt
     5761 tttctcccgc ttttacttca tctcctacat tcacaaatac ttcaaatcca tttccttcta
     5821 aatttacagt ttctatccct aaatgtataa gtatttctac tccacttttt tgggatttta
     5881 ttattattgc atgcttagtt ggaaatataa ctccaatagt tccatcaact ggtgaataaa
     5941 tcaatccatc actagattta attgcaaatc catcccccat cagtttactt gaaaatacag
     6001 aatctggcac ttcttctata tctaatatct ctccatttgt taatgaatca atcttttcta
     6061 ctaaagtatt ttccatattt gcaacttcca tatttttttt cactttattt ttatcaacat
     6121 ttttagtaac agtactttca acctttgttt ttccctctat tatatctttt atatcagact
     6181 ttattgaatc tgattttggt ccaaatatag cttgaacatt gttgccaata accataacac
     6241 ctgctgctcc aagtgatttc aatttttctt tatctacttt atttatatca tttacaacca
     6301 atctaagtct tgttatacac gcatctaggc tctttaagtt gtctttattt cctaaagcaa
     6361 tcaaaatctc tgatgctaat tcattcttag acatttcaat gacttcagcg ttagtttctg
     6421 actcatcttc tcttcctggt gtctttaaat taaactttct tatcataaat ctaaatacac
     6481 tatagtataa tactgcaaaa ccaataccta ccaagatagc caagtaccat ggagttctat
     6541 taggaagaac accaaataat aagaaatcta ttaatcctcc tgaaaatgtg aatccaattt
     6601 taactcctaa taattgcata attgcgtatg ataaacctga aaataaacaa tgtattgcaa
     6661 ataatactgg tgcaacaaat aaaaacgcaa actctattgg ctcggttatt cctgttaaaa
     6721 aagaggttaa tgctgctgat gccattatac ctgctacaaa ttttttcttt tctggctttg
     6781 cttcatgcac catagcaaga gctgctgctg gaagaccaaa catcataaat ggaaatgcac
     6841 ctgtcataaa agtacctgct gtgaaattta ctccatcttt aagttgagca aagaatatct
     6901 tttggtcacc cattacaagc tcaccagctt tatttacata ttctccaaat tgataccaaa
     6961 atggattatg gaaaacatga tgcaaaccaa atggtattaa tagtctttcc aaagaacctt
     7021 ccataaatgc tgctagtgtt tgatttgctt ctataacatt ttttgagaaa ctaagtaatc
     7081 cctcttgtat tggaggccaa ataaaagaca atataattcc tattgctata gcaactaatg
     7141 aagtaacgat tggtacaaat cttttacctg caaaaaaacc tagatactga ggtaattcaa
     7201 ttttataaaa ctttttataa agataagcag ccactatccc tataataata ccaccaaata
     7261 cccctgtttg aattgttggt attccaacaa tatttgcata catagaattg tctaccatat
     7321 cttctgtaac acccgccata acacccatag atgtattgag aattaaaaaa cctactatgg
     7381 ctgccaatgc tgcaacacct tctccttcag ttaaaccaac agctacccca actgcaaata
     7441 taagtggtaa gttattaaat ataatttgac ctgaactttc cattatactt attataagtt
     7501 gaaatgtagg attcgccaaa actggaaaat gagccaacaa tgcgtctcct gctaaagcat
     7561 tactcacgcc aagtaatata cctgctgcag gaagtaatgc aactggtagc attagagatt
     7621 ttcctacttt ttgtaaaaca ccaaatactt ttttcattta cttttctctc cttattattt
     7681 tatatttttt taaataaaaa agacttaagt ttataaaaag ttacattagt atacttaatt
     7741 ctaaaattaa acttatgtta atataaataa acctatatta acctaatttc agaattaaaa
     7801 ataaactatt ataacttttt tataaacata agt
//
LOCUS       DAELJO010000171         7766 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELJO010000171 DAELJO010000000
VERSION     DAELJO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.173"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            355..1674
                     /locus_tag="KPB88_003096"
     CDS             355..1674
                     /locus_tag="KPB88_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0055337.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(1749..2177)
                     /locus_tag="KPB88_003097"
     CDS             complement(1749..2177)
                     /locus_tag="KPB88_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055338.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2268..3644)
                     /gene="hydG"
                     /locus_tag="KPB88_003098"
     CDS             complement(2268..3644)
                     /gene="hydG"
                     /locus_tag="KPB88_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG0055339.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3645..4730)
                     /gene="hydE"
                     /locus_tag="KPB88_003099"
     CDS             complement(3645..4730)
                     /gene="hydE"
                     /locus_tag="KPB88_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG0055340.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTLVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(4766..5974)
                     /gene="hydF"
                     /locus_tag="KPB88_003100"
     CDS             complement(4766..5974)
                     /gene="hydF"
                     /locus_tag="KPB88_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG0055341.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(5989..6240)
                     /locus_tag="KPB88_003101"
     CDS             complement(5989..6240)
                     /locus_tag="KPB88_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG0055342.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSDFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(6621..7661)
                     /locus_tag="KPB88_003102"
     CDS             complement(6621..7661)
                     /locus_tag="KPB88_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG0055343.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         2654 a         1183 c         1082 g         2847 t
ORIGIN      
        1 aaacataaaa aacaacaaga gcaactagaa cattttaaaa aaattattaa tgagaaaagg
       61 taattataca atttaatgaa gacgaataga ataatggtat ttataagatg attttcagag
      121 gactctataa aaatagaata gagtccttta ttttttgaaa aataatgttt atttgataaa
      181 aataaaatat attgtttctg aataacaaat attttgaaaa ttataaaaac atatattgac
      241 aatttgtgtc aaaaatacta acattaagta tataaagaat tttatatgag aatatcttta
      301 taaatcgcaa taattttaaa acataaattt atttaaagag gggatgattt gccaatgagc
      361 gagataacta gtcaaatgat aagtacagaa gaaaaaaaat atgttgctaa aactcaaaag
      421 ataccttatt atccagtagc atttaaatca ggtgatggtg caatgctata tgattatgaa
      481 ggtaatgaat atgtagattt tttagcaagt gcaggctctg caaatgtagg tcatggtaat
      541 aaggaaatct cacaagcagt taaagaacaa atggatgata taacacaata tacacttgca
      601 tatttccata gtgatcctcc tgtaaaatta gctgagaagc ttgtggaaat agcccctgga
      661 gataatgata aaaaagtact atacagtgca actggttctg cttgtataga tgctgctata
      721 aaattagcaa gagggtatac tggaagaaca aagataatat ctatgtgtga atcttatcat
      781 ggaagtactt atggagctat atctatatct gctttaagta caaatatgag aagaaaaatg
      841 ggtccattgt taccagaagt ttatcacttc cattatcctg ataaaaatag aacagctaaa
      901 gaatgtttag atgagataga atatgctttt gcacactatc taccagcaga agaggtagct
      961 gcaatattta tagagccaat tgcaggagat gcaggaataa tagtgcctcc agtagagtgg
     1021 gtacaaggtt taagtaaaat atgtaaagaa aatggaatat tacttgtaag tgatgaaata
     1081 caacaaggta tgggaagaac aggtaagtgg tttggtatag aaaattttgg tgttgaagca
     1141 gaccttatag ttcttggaaa atctgttgga ggtggattac ctctaggagc agttgttgga
     1201 agaacagaaa taatgcaaag tcttgatgca ccagcacatt tatttacttt agctggaaac
     1261 acaacagtat gtgtggctgc tttaaaatct atagagataa ttgaaaaaga aaacttactt
     1321 caaaagagta tagaaatggg agattatatt aaagcaggat ttgaaaaact aaaagaaaaa
     1381 tacgatataa ttggagaaat aagaggcata ggattatcta taggagttga tatagttaaa
     1441 ggaaaaggtt ctaatgaaaa acatccagat gctacagcta agatatgtta tagatgtata
     1501 caaactggat taataatgat attcttagga caatctactt taagagtgca accacctcta
     1561 gtaataacta aggagcaagt agataaagca atgaatatca tagattctgc aatagatgat
     1621 tatttaaatg gtagaattgg tgatgaagta tatgaagtaa ctcaaggttg gtaaaataaa
     1681 tattaaatga aaataggagt tatctcaatt ttagaaaatt atttctgaaa agagataact
     1741 cttttaagtt acccacaaat aagaaaattg tttgattcat taaagactat atttcggatg
     1801 tcgtttttgt gagtatcaga gacagtatta cgagaataat tacctttatt cgtactatct
     1861 gttaatttag ttataattaa gatagctaaa agaataagaa ttactgtgta taaagcaata
     1921 acatatggtt ttcttttata tttgtatttg ttattgaaaa aagaatggcc ataattattt
     1981 ttatttactt tagatgtatt tgtattcttt ttcttgttac ctttcttttt atcatccttt
     2041 tttctatttt tagggtaagg aatgactttg tgtgtattat caagtttgat atttttattt
     2101 ttttgaatat tgttttctag gctagccttt ttttctccaa attgaatagt tttattggta
     2161 ttgttgttat ctttcaatgt taatctccct cccttaaact ttccctttag ataatattac
     2221 tactaaccta tggaatatac aataaataat atataagtaa taaaatttta taggtataaa
     2281 tctctttctc cacgctctaa ggcagtcata ttgttagata ctatgtttct tatatcttct
     2341 ctttctatct tacctatttc ttctgcgata agttttttac ccataatttt aagttcttca
     2401 tctccataat cgattaaaaa ttcatttaat gtagtaagag catttggtgt acatacatta
     2461 tggatttgtc gacttttagc taagctcatg aatctatcgc cagttctacc tttacgataa
     2521 caagctgtac aataactagg tatatatcca tcagtaatca gttctttaag cacctcaaca
     2581 gggcttctat ggtctccaac ttcgaattgt tctgtattgt caccatcttc atatgcttta
     2641 tatcctccaa cacctgttag agaacctgca ctaatttgtg atacaccata ttttagtaac
     2701 tcatttctca tttcagcagt ttctctagtg gacattatga ttccagtaaa aggaacagct
     2761 aatctagtta tagctactat ttttttgaac atatcgtcag aaactaagtg aggaaaatct
     2821 tctaagctca taccttcagc ttttttcaat ctagggaaag atatagtatg gaaacctaca
     2881 ccaaattttt cctctaagtg ttcgttgtgc atcataagtc ctagaacttc aaatttaggg
     2941 tcagataaac caaataaaac tcctgcacca acatcatcaa caccagcttc catagctcta
     3001 tcaaaagctg ttaaatggta ataatagtca ttttttatag attgaccatg catttttata
     3061 aatgttggtt tgtgataagt ttcttggaat aatatataag ttcctattcc tttttctttt
     3121 agtaatttat actcatctac acttgtagct gctatgttaa cgtttaccct tcttatattt
     3181 ccgttttcat tatatgttga ataaattgca tctaagcaat ctaagatgta atttatatca
     3241 caatttttag ggtcttcacc agcttctaga gcaagtcttt tatgacccat tttttctaat
     3301 attttaacct cttcccttat ctcttccatg gtaagttttt ttcttttaaa tttgttgcat
     3361 ttactaaaac cgcaataaac acattcattt acacagtagt ttgatacata aagaggggca
     3421 aataaaacca ctctattacc atatatttca ttttttattt gcccagctat actaaataat
     3481 ctatctagtt gttttttatc ttctacttca agaagtgtag ctatatcttt ataactaagt
     3541 ttttcccttc tatcagcctt atcaagtacc ttttcaatat catcagaagt tgaatttttt
     3601 gcatcttcta ataaagaatt aataagttca tggtttatga acatttacaa acgcctccat
     3661 tcaatattat caccgcgaga atagtcaact tcaagtccta atgacttgat gttttcttct
     3721 aaactttggt gatattcaca agcttcttga ccagtaaagg ctttgttatc gtaaagagaa
     3781 tattgttttc taaattcttg aggagaaagg tttggcataa ttacattgca accagctaaa
     3841 agacctgcat ttctaccact agggtttata gatgccaagg ctgttgtagc gggtaataaa
     3901 acttttggaa gtagaagtct tgtaattgaa agcataagta atgtcttctc taaatctcca
     3961 tgtttataat ccttaaaaat tgtatcatgg tgtggaatga aaggtcctat tccaaccata
     4021 tgaggattta actcttttag gtataataaa tcacgaacaa ggtcttcgtt gttctgaaat
     4081 ggtgattcaa ccataaatcc agcacctacc tgataaccaa tttctttaag gttttttagg
     4141 cattcttttc tagttctaag ctcaatgttt ggcggatgta attttttata atgactgtct
     4201 gtagcagttt catgtcttaa aagatatcta tctgcaccac ataaaaagta ttttttatac
     4261 gaatcataac tcttttctcc tagtgataga gttatagcac aatcctcata tttatttttt
     4321 atacttgaaa caatttcaca tattttatca tcagtaaaat atgcatcttc accaccttgc
     4381 aaaactaatg ttctgtatcc tattttataa cctatatcac aacattcaag tatttcatca
     4441 agtgaaagcc tgtatcttat gatattttta ttagaagatt gtattccaca atagtagcaa
     4501 ttatttttac aatagtttgt aagctctata agtcctctca agtagactct atttccataa
     4561 tgtttatttc tcatgtcgct agcttgttta taaagatagt ttcttaaatt ttgttcgaag
     4621 ttatattcct cattgtcgat attttctatt aaatatagta gttcatcaaa acttaatgaa
     4681 ttaatgctgt aaagtttatc tataaagtat ttaatcttat ttttagtcat attcaaatat
     4741 gtaattatta agtatagtta aatttttaat aattgaaaag ttcaagtgat ctttccaaaa
     4801 taccttttac atatgctatt aagacaccat agtttacaat aggtacattg aaagattttg
     4861 ccttttctat tctagataac attccagctc tattcatcat acaagctcca cagtgaacta
     4921 caagggcata tttatttata tcttcagtga aggatactcc agaactaaat tcaaaagtaa
     4981 tgtttttgcc agttttcttt ctaatcatat taggaatttt tactgttcca atatcatcag
     5041 tttgtctatg atgggtacat ccttcggcca tcaagatttt atcaccatct tttaaatttt
     5101 ctaaagcata agctccatta atcaattctt taaggtctcc cttttgtcta gcaaacaata
     5161 tagaaaaaga tgtaagagga atatcttttg gtgtatcttt atctacttgt ggaaatactt
     5221 gagagtcagt aataacaagt tttggttttt tgcctaaatt agatagagtt tcttttaaac
     5281 tatcttcttt tgtaactata gaaattgcac cattatccaa aatatctctt ataacttgct
     5341 gttgaggaag tataagtcta ccttttggag ctgctttatc tattggaaca actaaaacaa
     5401 ctaaatcatt tggttcaatt aaatctgata ccaatttgaa ctcagtgcta tccttaggca
     5461 aaacttttat tatttcattt tttaaatttt caattccaat tttatctgtt gatgagacag
     5521 aaacaactgg acatttaact ttatttttag ttaagtttaa aatttctgat tgattcttaa
     5581 tagtatcaat tttgttaaga attagaatat gagggatatt cttatcatta aatttttcaa
     5641 ttagtgacaa atcttcttgt gatataccaa tttggcaatc cactactagt aaagctatat
     5701 ctgtcttttc taaaacatct agtgatttac ctattctaag ttcaccaagt tcaccaacat
     5761 catcaagacc agcagtatct ataagaacac aaggtcctat aggaaggatt tccattggtc
     5821 taaatactgg gtctgtagta gttccagcaa tatctgagac tattgcagca ctttgatttg
     5881 ttatagcatt tattatgcta gatttacctg catttctttt accaaagaga cctatatgta
     5941 ctcttaccga ttgtggagta gaatttagac tcatttgtac tcaccacctt aaacttcttt
     6001 ttttgatata ctagttttta cagatacatg aggtatgttg cccaatttac cagtcaaact
     6061 attaatagta tccatttcgc caacaactgc tatacatacg attgagacac cttcttcttc
     6121 gcaaggaatg cccattcttc ctttaataat acctttaaaa tctgatacaa cctcattgaa
     6181 tttaaattga cattcttttg gctcttccaa aatagcactt ataactgcaa cctttttcat
     6241 aaatatcctc ctaactcaga aaatatgtct taaacaaatc caaaatcaaa aaacttttct
     6301 ttgtaaagaa aagtttctac aaaagaaaag ttattaatta cacaagtata atgtgctata
     6361 ttaaactatt tccttacaga ttagataact cttttctggt agggttagct tgtaaaaatt
     6421 taatttaaca acaagtaact gaagaaaatt tttatacaat ttatataaat tataataaaa
     6481 attaactaaa gaaaattgaa gttacttata taatagtagt aaattgaaat taagtctata
     6541 gaaaaaccaa tttttgtata aaaataaaaa ttaaagttaa ttatttgtta agaattgact
     6601 ttggtaaaat atataattaa ttattcttta agcatataat ttataagtaa ttttagtgta
     6661 ttttcaatag cttctatatg tgttctttca taatggtgac tattgtttgt tccaggacca
     6721 acacatgcta tttgaacatc ttctccccaa cgaatagctt ctgaagcatc tgaaccatag
     6781 aaagtaaata catcgacagc gtagtctaaa ttattgtctt ctgcaacatc aacaagcttg
     6841 tttcttaaat taaaatcata taatgataaa ttatcttttg caactatagt tacaccatgt
     6901 tcactagatg tttgacttgg tccagtaggt gctatatcta ttgctacaaa ttcttttgtt
     6961 ttttcaggta aacatttaga aacaccatgt ccaatttctt cataattact tatataaaag
     7021 ttagtagtat gcttaggtgt taaattgttt tctttaaaat atctacatat ttcaagaacc
     7081 attgctacag ctgatttatt atctatatat ctagatttta taaaaccaga ttctgtagcc
     7141 tcaaatctat ggtctaggca tacaaaatcc ccaacactta ttcctaaatc taatacatct
     7201 tgctttgtaa atactttttc atcaagtcta ataatcattg tttcttcatt tctttcagtg
     7261 gcagcttttg tttgaccata tatatgagta gaagcatact taaatacaac acttcctctg
     7321 atttttttac cttttctagt tataacagta cagttttctc cctctatagc tgcccaacat
     7381 ccaccaccaa ctttatggta tttaagagta ccatcagatg atatttcttt tactatagct
     7441 ccaagtgtat ccatatgtgc agatattgtt atttgatttg aatcatcttc accaggaaga
     7501 gtagctataa gtgcaccttt ttttgtaaga atagtgtcaa gacctaaatt ttcaaaatct
     7561 tttttgattt ctaaaactgc gttttcagta tatccagctg ggcttggaat gtccaaatat
     7621 tttttcatta aatttatagt attagttaaa tttaaattca tattgtttcc tcctaaataa
     7681 atatatttat acaaattata acataaatac aaatgttctg aaaactaaaa ttaaaagaat
     7741 agaatctaaa catatttaaa aaaaaa
//
LOCUS       DAELJO010000172         7670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELJO010000172 DAELJO010000000
VERSION     DAELJO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.174"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            38..370
                     /locus_tag="KPB88_003103"
     CDS             38..370
                     /locus_tag="KPB88_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG0055344.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            532..717
                     /locus_tag="KPB88_003104"
     CDS             532..717
                     /locus_tag="KPB88_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055345.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            744..1829
                     /locus_tag="KPB88_003105"
     CDS             744..1829
                     /locus_tag="KPB88_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0055346.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHT"
     gene            1822..2280
                     /locus_tag="KPB88_003106"
     CDS             1822..2280
                     /locus_tag="KPB88_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055347.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2315..3406
                     /locus_tag="KPB88_003107"
     CDS             2315..3406
                     /locus_tag="KPB88_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055348.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3411..3635
                     /locus_tag="KPB88_003108"
     CDS             3411..3635
                     /locus_tag="KPB88_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055349.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3645..4208
                     /locus_tag="KPB88_003109"
     CDS             3645..4208
                     /locus_tag="KPB88_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055350.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDVFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4169..4798
                     /locus_tag="KPB88_003110"
     CDS             4169..4798
                     /locus_tag="KPB88_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055351.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4838..4975
                     /locus_tag="KPB88_003111"
     CDS             4838..4975
                     /locus_tag="KPB88_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055352.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            4975..5727
                     /locus_tag="KPB88_003112"
     CDS             4975..5727
                     /locus_tag="KPB88_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055353.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5740..6225
                     /locus_tag="KPB88_003113"
     CDS             5740..6225
                     /locus_tag="KPB88_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055354.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6232..6381
                     /locus_tag="KPB88_003114"
     CDS             6232..6381
                     /locus_tag="KPB88_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055355.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6517..7338)
                     /gene="yidA"
                     /locus_tag="KPB88_003115"
     CDS             complement(6517..7338)
                     /gene="yidA"
                     /locus_tag="KPB88_003115"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG0055356.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         2943 a          849 c         1359 g         2519 t
ORIGIN      
        1 aattttgtaa aatataaaaa gtataggggg aataaaaatg aaagtagctt atatttttag
       61 tagtactaat acacataaaa ttctagacaa aatgataata ccacaacttg aacaaggaac
      121 tcatggagta gacgttctag ggatgttttt ctttatggat aataccttat ttttatctaa
      181 aggtaatcct gtaggcgaga gactaagtaa attacatgaa aaaactgata tgataataat
      241 ggcttgtgac caatgtgcta tagaaagagg tatagagaat aatttggttg atggagctac
      301 aattggatgt ttcccaaacc tatatgcttg tttaggcagt gcaggtgtgg accaagttat
      361 aacgttataa attagtatag gatacaatcc atagaaaaat ttattttctg tggattattt
      421 taagtaaaag ataaattgtt taaagtaggg gatgaacaaa tgtctaaaat tttagttgaa
      481 tttataaaca cttgaccatc ttgtgatgaa catggagtgt ttataaaaaa aatgtcagaa
      541 aagtatgctg ataaattgga aataaaatta tatcaagcag ggaaagattt ttcgtatata
      601 aaaaaatatg ggattataac aaaaggaact ttaataataa atcagaaaaa gaaatatgat
      661 agattaaata aagatacaat tgaaagggca atagtagaag ctataaacaa taattaaaag
      721 tttaaaaatt aggagtatca aaaatgaata tattagaaat tgttttaaat ggagttaaag
      781 atactatagt agcttctata gttacactga atggagcatc aggatggctt atgataagtt
      841 ttatattggc aggtgtactt cacaatgttt taactccaag aaggtttcaa aaacaacttg
      901 gaaataagaa attttcttct ttaattaaaa gtactatctc aggaatgttt ttaccaatat
      961 gtagttgtgg agtaatacca cttggaataa gtatgtatta ttcaggagca tatttaggac
     1021 cagtacttgc ttttatgaca tcaactccaa taataaatcc aatagctgta gttctttgtc
     1081 taggtctttt aggtcctaaa gttacaatca tttatctaat agctggtttt ttagtaccat
     1141 ttttagtagg atttttagga aattgttttg gaaaagaaga attatcactt cataatgatt
     1201 cagaggaaga agtaatagaa ttagaagaag aaaaaagaag ttttatggga aatatgattg
     1261 atggtcttaa atggtctttt ggtgaattag cccttactat tagtaaatat gtagtaatgg
     1321 gtatggtttt tgcaggattt ataacaacta tttttccaaa tagtataatt caaaaatatt
     1381 taggaaatcc aggtttgcta tctcttggaa gtatagcaat acttgcatgt gttatgtatg
     1441 tatgtgctgt aggacatata ccgtttatag cagcccttgt tgcaagtgga gcatctcctg
     1501 gtatagcgat aacatttcta atagctggag cagctacaaa tctaccagaa cttataagta
     1561 tgtttaaact tattggaaaa agaactgtta ttatatattc tgtaactttg acactgtctt
     1621 caataataat tggatatata acaaatttac ttttagtgaa taataactca gtaaatttag
     1681 atgcaacaaa ttcttcaata cagttggcaa ataaactaat gttaaacata cctgatagtt
     1741 ttgagtatgt atgtactatt ttaattattt tatttgcatt taaatcaata ataccaaaga
     1801 tgaaagaggt cataggaaat catacttaaa aagatagtta taatatcctg tatagtagtt
     1861 gtgttaatca ttttatcaaa aatagttgat gataacatca aagaagatgc ttctattcca
     1921 aatgtgaata aagaaacatt agaatatttt agaaaaaatt ataaagaaga cataataact
     1981 tgtgctgaag aagacttaaa taatgatggg aaaaaagatt tagtagttat atataaaaaa
     2041 agcaataatt caaatgaaat ggtagtcgta gtttctgata aaaatagcca ctatataaca
     2101 aagccaatac cagcacctat agagaatcaa actataacct ttaaaaatat tgatgataaa
     2161 gcaccaatag aagtaattgt atcagggtct aaaaatggta atgtaggata tgctatttac
     2221 agagtagagg gtaaaaaatt tgttgattta tttggcgaag atatggacaa atgttgctaa
     2281 aaataaaaat aatacataaa ggtgggaaat ttatatgaaa ttatctaaga agattcaagc
     2341 attaatctta tttggtgtaa tgggtacatc tatattaaca ggatgtagta gtaaaccaaa
     2401 agaaaaagaa gaagcaaaaa ctggaaaaga tgattataca ataacagtag gttattataa
     2461 ttgtgaccat atgactgcgg gaccagtagc agaatcggca ggaatatata aagatttagg
     2521 tcttaatgta aaaactgttg gaaatggtaa agttcctgaa gctatggcag caggaaaaat
     2581 ggatgcaggt tacataggaa ctaaggggtt agttggagca atacctaagg gttcgccaat
     2641 aacaatagca gctaacaacc atactggtgg ttctgaatat ttaatagttt caaaagatat
     2701 aaaagaacct aaagatttaa taggtaaaaa aattgcaaca gatatgagtg atttcttatg
     2761 gacaagtgat tatggtccag aaacaggtct accaactgac ccatcaaaat atgaagttgt
     2821 aaatatggat tcagacaaag ataaatattt agctcttaaa actgggaaaa ttcaagcttt
     2881 tacatcttgt gacccttggg gttctgtagc tgaaaatgac ggtgcaggaa aaataatagc
     2941 atctactcaa tataaagaaa aagctaatgg caaagaatat aactgttgtt cattctcatt
     3001 aaacaaaaac tttataaaag aacatccaga tttagcaaaa aaattggttc tagcacatac
     3061 aaaagcaata gaatatatat atacaaatcc agctgaagca gctaaaatat ttgcaaaata
     3121 ttataatgta gaagaagaag ttgcacttag aactatctat aagaagactg ttggagaagg
     3181 tagaactctt acttggaaag ttacaggtga agaatataaa aataatttac aaatgtataa
     3241 agatttaaag gctttagatg atatacctaa atatgaagat acgattgata caagcttatt
     3301 ggactcttgt ggagctgatg attttgacaa atttataaaa gaaaaagtta acccagagtt
     3361 tcctgaaggt atgagttatg aagactggaa agcaaaggta ttataaataa atggagaata
     3421 tgaaaaatat aataatactt tttatcatta ttttgggaag tatatttata atatttttta
     3481 attttgaaga taaagaagaa ataaaaggaa aattaagaat aggggtatct gatgatacct
     3541 ctggttttgt aataaattat atggtaaata gagactactt tgaaaatata aaagtaagtg
     3601 atataatgga tatttttaca attaatgatt gctgagcaag tacaatgcag tgggctctga
     3661 gttcagagga aatagatata gcaatcatat gtaaagatgc tgcaaaacaa tatgtaaatg
     3721 taaatagtgg atttgaaatt gttggtactg tagttcaaaa ttctgatgta tttctattaa
     3781 gtgataataa tcctgaaaag ataggagtca ttcaaaatag agattaccag tcagaattgg
     3841 ttaaaaaata ctacaagaat gtggaagtag ctcccttatt agggactgca ctagcatatg
     3901 gattagaaag taatttagta gatggtgttg taatagattg cataaagtca attgggttag
     3961 agggtaaaag aaaatctaca actaaattag gagattatga tacctatgta ttagttgtaa
     4021 ataaagagtt taaaaaaagt aaagcatata gtgattttat aacactttac aataaaagcg
     4081 ttgatgcttt aaagaataaa gctacattta agaaagtatt aaaagagtac aggaatataa
     4141 gaaatataga tatggaggaa ataagcaatt ggaaattaaa atttctaaag ataaaagcga
     4201 tagattaact aagaaaaaaa ctgatggtag tatggatatt ttatataaaa taatactatt
     4261 agttattata tttttagtct ggcattttgc agcaaaagat atagggagtt ctttgttact
     4321 tccaatgcca gtagatgtaa taaaaggttt ctttttctgt gtgacagatg cagagacagt
     4381 cactaatctt tttataacat tgcagagagt acttaaaggt tttatgtatg cattattgtt
     4441 tgggcttcca ataggattta ttatgggatt ttctaaaact tttgaaaggg ttttatctcc
     4501 agtagtagat tctgtaaggc aagttccaat aatggcatgg gttcctctta ctatagtatg
     4561 gtttggtata ggagatggtc caacaatatt tttaatagct ttttcagggg ttttccctat
     4621 aattttaaat actattcaag gagtaagagc aatatcaaag gattattata atgcagctag
     4681 aagtatggga gcatcgccaa ttgtgatatt tacaaatgta attgttcccg catctcttcc
     4741 agatatacta actggttcaa ggattgctat aagtacagga tggatgtctg ttatctgagc
     4801 cgagtttata gctacgagtg ccggtctcgg ttactcaatg gtacaagctc aaacattaat
     4861 gcatacagat gtattaattg ggcttatgat atttgcagct ttaataggat ttataataga
     4921 tagggtatta aagtttataa ataaaatcct ttgtagatgg aggtttgcag attaatgaag
     4981 ttaagtgtaa gagatataaa taagacattt gtaaataata gagtacatac aaaggtttta
     5041 gaagatataa gtatggatat agatgatgga caatttgtct gtcttttagg accatcaggt
     5101 tgtggaaaaa caactttact tactataata ggtgggtttc aaaaatcaga acgtggagat
     5161 gtttttataa atgataaaag agttaaaaaa ccgggcatag atagagcttt tatatttcaa
     5221 aattatgctt tatttccttg gaaaactata agaggtaatg ttctatttcc tatgaaacag
     5281 cagaaaatac caaaagaaaa aagagaagaa atgctagaag aacttttggt tatgtcagac
     5341 ttgaaaggaa aagaaaaact gtttccgcat caattatctg gaggaatgaa acaaagagtg
     5401 gccatgataa gagctttagc atgtaatcca gaggtattac taatggatga accattagga
     5461 gctgtagatt ttcaaatgag acaaaattta caggaagagc tagaacgaat atggataaag
     5521 aagaaaataa ctgcattaat ggtaactcat gatgttgatg aagctgtata tatgagtgat
     5581 agagttatag taatgtcgag agataaagga agaattattg atgatataaa tatagatatt
     5641 ccaagaccta gaattagagg aagccaaaaa tatgaagagt acaaaaacaa attaactgat
     5701 actttatcaa agtgttatga agtttaaaga ggagtagaca tgagtcaaat agaaaaatac
     5761 aatagtttgg ataatacaca acagaaaata agaatctcta ttatggatat gataatagat
     5821 aaaggttcta gtgttacact tcaagaagtg actgaatatg taagtaaaaa gttgaatata
     5881 gaaaaagaat atatagaaag gacactacaa tattttattt ataaaaatat aatggttgtt
     5941 gatgggaata gtataaattt tatatatcct gtatcagcat tgccaacaaa tcataaagta
     6001 actttgagag ataatagaag tttcagtgca atgtgtgcta tagatgctat aggaacctct
     6061 tgcacattca accaagatat aaaaataaac tctatttgta gtgctacagg taaggaaata
     6121 gaagtaatta taaaaaatga aaagattgag tatgtgaata atccaaactt aagagtgctt
     6181 catataaatc ttgataagca tctaaattgg gctgctagtt gctgaaatat aatgaatttc
     6241 ttctggacga agagtgattt tgatgcttgg acaaacgaag cgggattatc taatgatgaa
     6301 gatatatatt gtttagatat taatgaggca atagtagaat catataaaat atttaaactc
     6361 aaacaaaagg tactatcata gatatacata tatgaaaaac taaaaatgac ctttagataa
     6421 aaatgatagg taatatccat actatataaa ttagtataga gtagggatgt gaaaagtatt
     6481 taaatctaaa ggtatatttt tagttttttt agatgattag atagccttat taagtatata
     6541 tttatttata acctttgcaa ctccattttc attattagta tcagtaacat aatctgcaat
     6601 ttcctttata ctattacgtg cattccccat agccacacca agacctgcat actttatcat
     6661 atgttggtca ttttcttcat cgccaacagc aatagcattt tctataggta tatttagatt
     6721 cgtacataat gttttaagag cagttccttt attagtagtt ttacctaaaa attctagata
     6781 aaaaggagca gaccttacta tagtatattt attgaataaa tttttaggta gttgctgtat
     6841 gatttcttcc aatctttctg gttcatcaac tatcataatt ttacagattt taatagattc
     6901 atcaatttta tctttttgta tatatttgac ttctatatta tttaatttcg cttcatgaag
     6961 tgtatatttg ccaggcgttt catttggtgt aattaaattt gtcattgtat ttatgtgtat
     7021 tttagtctta tattttttac aaacgttatt atatataaaa tcaaaatcat ctaaagtcat
     7081 ttctacatca tgtatgattt tctcactttt aacttcttga ataattgctc cattaaagca
     7141 tataacataa tcaccttcat ctgttaaatt tagttgattt agaagttctt ttacaccagg
     7201 aagaggtctg cctgttgtta taactacttt tgccccagcc ctttttgcct cctgaatgga
     7261 ctcaaagact tcaggagtaa tccttttttg tgtatttaat attgttccat caatgtctag
     7321 tgcgattaat ttgtacatat caacctccaa taaattatat actcaaattt cctgtgaaaa
     7381 cgttgacaca gctagtgttg aacaaagttt cagtgggaaa tatggtttgt tttgtatact
     7441 attatatcaa gagataagta atttagaaag tttaataata ttaatttttt gataaaaata
     7501 ttacttatgt gagatgagaa tgtaaaaact ttgtaatcaa taattccaaa attcttaatt
     7561 acaaataatg gtatcgtttg cattgttaat ataaataaat gtatattata tagttagaag
     7621 tttgaataaa tagactgatg gtaatttgtt atattagtta gttttgtgtt
//
LOCUS       DAELJO010000173         7652 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELJO010000173 DAELJO010000000
VERSION     DAELJO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.175"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..289
                     /locus_tag="KPB88_003116"
     CDS             <1..289
                     /locus_tag="KPB88_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0055357.1"
                     /translation="CKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFEKLEVYFVT
                     NTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQLT"
     regulatory      382..474
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            574..1368
                     /gene="thiD"
                     /locus_tag="KPB88_003117"
     CDS             574..1368
                     /gene="thiD"
                     /locus_tag="KPB88_003117"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG0055358.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1392..2180
                     /gene="thiM"
                     /locus_tag="KPB88_003118"
     CDS             1392..2180
                     /gene="thiM"
                     /locus_tag="KPB88_003118"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG0055359.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            2203..2835
                     /gene="thiE"
                     /locus_tag="KPB88_003119"
     CDS             2203..2835
                     /gene="thiE"
                     /locus_tag="KPB88_003119"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0055360.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2974..3276
                     /locus_tag="KPB88_003120"
     CDS             2974..3276
                     /locus_tag="KPB88_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0055361.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3342..4091)
                     /locus_tag="KPB88_003121"
     CDS             complement(3342..4091)
                     /locus_tag="KPB88_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055362.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIILIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4085..5047)
                     /locus_tag="KPB88_003122"
     CDS             complement(4085..5047)
                     /locus_tag="KPB88_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055363.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5051..5509)
                     /locus_tag="KPB88_003123"
     CDS             complement(5051..5509)
                     /locus_tag="KPB88_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0055364.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5865..6221
                     /locus_tag="KPB88_003124"
     CDS             5865..6221
                     /locus_tag="KPB88_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055365.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6293..7009
                     /locus_tag="KPB88_003125"
     CDS             6293..7009
                     /locus_tag="KPB88_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055366.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7002..>7652
                     /locus_tag="KPB88_003126"
     CDS             7002..>7652
                     /locus_tag="KPB88_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055367.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIA"
BASE COUNT         2974 a          892 c         1253 g         2533 t
ORIGIN      
        1 atgtaaagat gattctctat acactggata taccagtaat atagttagaa gaatgaatga
       61 acataaatta ggaattaatt caaaatacac aagagcaaag ggatttgaaa aactagaagt
      121 ttattttgtg accaacacaa aaagcaatgc tatgaagtta gaatactata taaaaaaatt
      181 aactagaaat aaaaaattgt ctataataaa aaacccaagt atacttataa atttaattga
      241 taataaagaa gattatatta ttggtaagga aatagaacaa ttaacataaa tatttatagt
      301 aacattataa tgggtgcata tggtgtaaaa acttgacaat aaatactatt gtaatataat
      361 gaagttataa atatagtaaa ttaaaatagc taggggagcc aagagctgag aggtataccg
      421 acccttacac ctgatctgga taataccagc gtaggaaagc ttagtaaaaa ttcaagtatg
      481 tattatttgt gatatatgct atttctaagc tatacctatt gtaggtatag cttttttaat
      541 taagttattt aaattaaact ttaggaggca acaatgagta attataaaat accaacactt
      601 acaatagctg gatctgattc atctggagga gctgggattc aggctgattt gaaaacgttt
      661 agtgcaattg gtacttatgg tatgagtgta ataacagcta taacagcaca gaatactaaa
      721 ggagtttttg ctgtagagga tttaaataag aaaataataa aaaaacaaat agaggcagtc
      781 tttgaagata tacctccaag agctgtaaaa ataggaatgg tttcaagtcc tgagattata
      841 cttgaaatag ttgagaatct aaagaaatac aatccaaaat atttggttgt ggacccagta
      901 atgatatcta aaagtggata ttatctatta aagccagagg caaaagaaaa cttaattaag
      961 tatttgatac cacttgcata cataattaca ccaaatatac cagaggcaga agaaataaca
     1021 ggaataaaaa tacataatgt agatgatatg aagagagtag gagaagaaat actacaactt
     1081 ggacctaaat ttgtacttat gaaaggaggt catttagatg gagaagcagt ggatatttta
     1141 gttggtaaaa atatatttaa agtttataaa agtgaaagaa ttgataaaaa aaatacacat
     1201 ggaacagggt gcacattatc ttctgcaata acatcttatc tagcattagg atatgaaata
     1261 acagaagcag taaatctaag taaaatctat ataacagaag caataaaaag aagttttgat
     1321 ataggtcatg gagtaggacc agtacatcat ttttataaat ttgagtaata aaaaaatatt
     1381 ggaggtagaa tatgtataat ttaattaaag atgtaaaaaa attaaatcca ttggtaattc
     1441 attatactaa taatgtaact ataaatgatt gtgctaatgt aactttagct gtaggagcaa
     1501 gtcctcttat gagtttttca tatgaggaag tagaagaaat ggtaagtgta gcaaactctg
     1561 tagtaataaa tataggaact atgaactcaa atatgttgga tttattccta ttagctggaa
     1621 aagcagcaaa taagtacaat aaacctgttg ttttggaccc agttggagta tttgcaagca
     1681 aagcgagagc agaacttaca agtagacttt taaatgaagt caaatttagt gttgttaaag
     1741 ggaatgtatc agaaataaag tttataggtg gctttaatgt aaggggtaaa ggtgtagatt
     1801 catttgatga agaagaagat tctactgaga taataagaaa gattgctgaa aagcttgaat
     1861 gtgtagttgt agcaactgga aaaatagata taataactaa tggtaaaggt acatataaaa
     1921 taaacaatgg aactgataaa ttaaaaggaa ttacaggaac tggatgtatg actgctagct
     1981 tgattgcaag ttttatggct gttactgaga atatattaga agcagcaaca atgggtgttc
     2041 ttacaatgtc tttaagtggg gaattagcta atttaaataa tccaccaata ggtacattta
     2101 aagaaaatct tatgaatgct atttatcaaa tggatataga tgctttaagt aaaaattcta
     2161 atattgaatt tttaaattaa taaagagtaa gggagactta aaatgattga taaagaatct
     2221 ttaaaaaaat gtttaaagtt gtatttagta actgactctg aaatgcttaa aggtagagac
     2281 ttttataaat gtcttgaaga tgcaatatct tctggaataa caactgtaca acttagagaa
     2341 aaaaatgctt ctggaagaga gtttttaaga aaagctatga aacttagaga aataactaaa
     2401 agatatggtg ttaagtttat aataaatgat agagtggata tagcacttat ttgtgatgca
     2461 gatggagtac atgtaggaca gagtgacatt gatgttagag aggttagaaa gcttatagga
     2521 aacaataaaa ttctaggagt atcagcaaga actttagaag aagctatttg tgcaaaaaat
     2581 gatggcgcag actatttagg tgtagggtct atattcacaa catctactaa attagatgca
     2641 aaatcagctt cgtttgaaac tgttaaagaa attaaagaaa aagtagacat gccatttgta
     2701 ctaattggag gtataaattt agacaatata gataaactta aatgtttaga aagtgacggg
     2761 tatgctataa tatcagctat attaaaagct gaagatatct ctaaagaagt ggaaaagtgg
     2821 acattaaaaa tataaaattg gctctggtag tgaaaaaaat ggaaaaattt gatataattt
     2881 aagagtaaaa tttaagcaat tataaatgat aaacttttct acatatactt tttaatcaag
     2941 atatatgtag atttggttaa aaaggagcta taaatgatta ggatattgtt agtgtgtgtt
     3001 ggaggcatgt cttctaccct attggtaaat aaaatggaga aggatgcaaa aaaaagaaat
     3061 atagactgta aaatatgggc agttggagaa ggagatataa aatcggagct tgataacttt
     3121 gatattttac ttcttggacc acaacttaga tttatgcttg atgacgtaaa gtctatagtt
     3181 ggagatagag ctcctgtatc tataatagat atggtaaact atggtacttg taatggtcat
     3241 gctgtgttaa attcggtttt agaaatatta aaataaataa taaaagtgga ttctatataa
     3301 tagagtccac ttttattatt tgttattaat aggcttaaaa cttagcttac catttctttt
     3361 tttaatctcc acatagcaag tgaaaaactt gaacagcaca atattaaaaa tagtaggata
     3421 acaggcagta tatctccaat aagaatagtc tcaccaaata ttaagctacg taaagcgtta
     3481 ataacatggg taaatggatt gagcatcact gctactttta atcctccaga taaactttct
     3541 atagggaaaa gagctgaact taaaaagaaa ataggaagta caattgcagt cataattgtt
     3601 tcataaacta tttcatttgg caataataaa cttatagaat aagctagact tgataaaaag
     3661 aaggctgtca taaatatcag tattatcata agtaggattc caccaatgcc agattctatc
     3721 cttactgaga agaatatact tactatacac aaaatagtta cttctatgaa agaaactaag
     3781 atagcttcta ataattgacc aaggacgatt gaatacctgc tgataggtgc aattaagact
     3841 ctatagaaac ttccactgtt cttcattata aagtttataa taccaccact actacaagaa
     3901 gaaaacacaa ccagtacaat gatacctggt agtataaaag cagtataatt attgatattt
     3961 atattattca tagtttggtt tgcaatagaa ctataaagca caagccaaag aaaaggttgt
     4021 aatatagtaa ctacaaatga gattggattt ttaaaacgcc acttcatact acgccataag
     4081 atatttaaca ttcccattcc tcctttaagc tttttttaga acttgtaagt gctaaaaata
     4141 catcttcgag acttggctct acaatttcta ttgcatcaaa ttctatatca tgttctaaaa
     4201 gccatttatt tattaaagta aaagcagttc tactatcgtt tacacttata aaaattgaat
     4261 tttctcgtac gctcataaac tttactaaac cagtactttt aattgaatct ttatattttt
     4321 ttatatcttc agtattatga aaagttatac gtagtatatt ttgtctaatg taactacgta
     4381 aacttgatgg tgttccttgt gctaaatcct ttccattctt cataatacaa atattatcac
     4441 tcaattgttc agcttcttca agataatgag tagttagaaa tatagttgtg ccatactcat
     4501 ctctaatttt taataacata tcccacatag attttcttga atcgacatcc ataccaacag
     4561 ttggttcatc taaaaatagg attttaggag atgataccat attcatagct atgtccaatc
     4621 tacgttttac accccctgaa tatgatgaag taggatattt taagtaacta gataagtcaa
     4681 acttatcaat taaagagtct attctttgtt ttgcaatctg tggttctact ttatacattt
     4741 tactttgaaa taccatattt tccataaggg ataaatgttc atcaatagag atttgctgtg
     4801 ctacacaagc aatttgagta cgtatccaag aaggattatc tactaaatct tttccaaaca
     4861 ttgttacatt tccagatgta ggtttgtaaa aggtagtcaa aatatttata agagaagatt
     4921 taccagcacc attaggacca agtaaagaaa aaatttctcc agcatttaca ttaaagctta
     4981 gaccatttaa agctttcaca ccatttttat attcttttac taaattgtct atttcaatcg
     5041 ctaacatata ttattcctcc tctttaagtg gaaatattat ctctgtaata tagttatctg
     5101 ggtttccttt tatcaacata cctggtcctt taataaatac ttctctccaa ggagtttgta
     5161 tttttatatt attttcatga atatatttaa atatcatttc atacgttttt ggcaaattac
     5221 tataagagcc tgtgtgagta aaaaataaag cttttgtttt aggaaattct tttgtagtta
     5281 tacccatacg atttggagtt tcagcagttg gaacacataa ttccatttct gcaacaccag
     5341 ttttttggtc aacatcatag tagcaaaaga atggtgcacc gttagacttt ccttttatag
     5401 ctttaaatac atttggaaaa tactttgttg cctcagtcat aggtccatta taacgcatgg
     5461 tagccactgt gactgattcg atatttttga tttcaatttt gcaattcatt tgtttacctc
     5521 ctaatgtgtg ttcatattct tactagattt ttaagcatta atacatataa gattttgatt
     5581 agttgaaaac atataatctt atctcgtttg cttgttttca agtctaacat aacataaaat
     5641 atatatcctc tcatttgatg ctccattttg taaagttatt atttaagctt tactttagat
     5701 tttttagcaa attaaaataa agcaatttat aaaataagta tttacaaatt aagtgtatta
     5761 atcgtataat acacttaata ggatactaga taaagtctga aataaaggcg tattttttta
     5821 aagtaagtgt actatcgtat taatacacta gaataggagg aatcatgaat tttgaattag
     5881 ataatacaac tcccatttat ttgcaaatag taaagtatat aaagagacaa atagtaacag
     5941 gagagttgaa accaggagag acaataccat caagaagaga aatggcactt aatctaaaag
     6001 taaacttaaa tacagttcaa agagcatata aggagatggg tgatatgaat ataataaata
     6061 cttttaaaaa ttatcaaagc agtgtaacag ttgatgaaaa tatattaaaa aatttaaaat
     6121 tagagcttat aaatgaatca ctatctgttt ttatagaaga tatgaaagca ataaatgtta
     6181 gcaaagaaga agttttaaaa ataatagaag ataagtatta gttttttaac aaatagaaaa
     6241 cttagtttag aaagtttatt tataagcata ttggatgtgg aggatagtaa atatgataga
     6301 aataaagaat gtcagcaaaa catataaaag gatgcaaggg cttaaaataa gaaagataga
     6361 agctttaaaa aatgtaagtt ttaatataga aaagggaaaa ataactgcat tacttggtat
     6421 aaatggtgtt ggtaaatcaa ctatgttaaa agcaatagca ggacttataa acattgatag
     6481 tggagaaatt cgtatagatg gagaaaaaat aaatgaaaag gtatacaata aattggcttt
     6541 tgttccagat gtacaatctc actttagcaa tacaacaata aaagaaacat ttgaatttat
     6601 ggaaattttt tattcaaagt ggaataaaga aaaatcaaaa gaaatgatgg acatatttaa
     6661 attggatgaa gatgaaataa ttgataactt gtcaaaagga aacattgcaa gagtaaaatt
     6721 aatacttgga ttttgccaag acccagagta catacttctt gatgaaccat ttacaggaat
     6781 tgatttgttt aagagggaag agtttatagg agtaatagcg caatatatgg aggaaaatca
     6841 agccattatt ataactactc atgagatagt agagatagaa agtttagttg atgaggttgt
     6901 aattcttgat gaagggcaga ttataacttc atttaatgca gaagaattaa gagaaagaga
     6961 aggtaaatct atattagata agatgaggga ggtatataag aatgaataaa cttctatatc
     7021 taataaatgt agatttgaga agaagcaata agttttatat agcttatata tcattttttt
     7081 cattaattac acttagttta aatttattcg aaattaataa atttaaaaat tctaaattta
     7141 ttatgaactt agcaatagaa gattttggag gtatatttta tggactaggg atacttagta
     7201 atcttggttt tatccaaagt atattgttct ttgggatatt gggacttttt atatactata
     7261 tttttatgtg gaaaagagag ttcttttcaa ataatcaaag tatttataca cttatgatgt
     7321 tacctcaaaa taagtttaag atttgtatat ctaaatcaat agccttgata acaatgatat
     7381 atggtttttt gataagtcag acagcaacat tatttatatg taaatatata tttaatttca
     7441 tttttagaaa tatgcctata atcaatatga actttgccaa agatttatat tatttaaact
     7501 tgaagagtat accaattgat tttatctctt ttatggctat ttatgtattt ttactactta
     7561 tagctatttc tattgtaaat tgctgtatat tatttatctt ttcctatata aataaatttt
     7621 atgtaatttt aatcactttg ctgattatag ca
//
LOCUS       DAELJO010000174         7513 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELJO010000174 DAELJO010000000
VERSION     DAELJO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.176"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            130..1323
                     /gene="nifV"
                     /locus_tag="KPB88_003127"
     CDS             130..1323
                     /gene="nifV"
                     /locus_tag="KPB88_003127"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG0055368.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1348..3273
                     /locus_tag="KPB88_003128"
     CDS             1348..3273
                     /locus_tag="KPB88_003128"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG0055369.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3295..4290
                     /locus_tag="KPB88_003129"
     CDS             3295..4290
                     /locus_tag="KPB88_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG0055370.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4616..5089
                     /locus_tag="KPB88_003130"
     CDS             4616..5089
                     /locus_tag="KPB88_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0055371.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5123..5617
                     /locus_tag="KPB88_003131"
     CDS             5123..5617
                     /locus_tag="KPB88_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055372.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5714..6235
                     /locus_tag="KPB88_003132"
     CDS             5714..6235
                     /locus_tag="KPB88_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0055373.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNHF"
     gene            6424..6735
                     /locus_tag="KPB88_003133"
     CDS             6424..6735
                     /locus_tag="KPB88_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0055374.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6763..7449
                     /locus_tag="KPB88_003134"
     CDS             6763..7449
                     /locus_tag="KPB88_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0055375.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKGKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         2991 a          749 c         1477 g         2296 t
ORIGIN      
        1 gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa aaataaaaaa ataatcataa
       61 gttagaggta tcaatgaaaa ataagactta atgttttaaa attaaaagta tactaagggg
      121 ggcattaata tgtgcgttat atcaaaagat agagcaaagg aaataaaaat agtagatact
      181 actttaagag atggagagca aacagctggg gttgtatttg caaatagaga aaaaataatg
      241 attgctgaaa tgttaagtga cctaggtgtt gaccaaattg aagttggtat acctactatg
      301 ggtggagatg aaaaaaatgt aataaagcac atatgtagta gaaatttaaa atcagatata
      361 atggcttgga atagagcagt aataaaagat gttgaagaat ctatttcttg tggtgttgat
      421 gcagtagcta tatcaatatc agtatctgac attcatatag aaaataaatt gagaacttct
      481 agaggctggg ttttagaaaa tatggctaaa acagttgaat ttgcaaagaa aaatggatta
      541 tatgtttcag taaatggaga agatgcttca agagcagata tagacttctt aactgaattt
      601 ataaatgttg gaaaacaagc aggggcagat agatttagat actgtgatac agtaggagtt
      661 atgaatcctt tttctataaa gaatgcaata gaaactcttt atgaaagaac taattttgat
      721 atagaaatgc atacacataa tgactttggg atggcaactg caaatgcact tgcaggaata
      781 gcagcaggag caaattatgt tggtgttaca gtaaatggat taggtgaaag agcaggaaat
      841 gcagcattag aagaagtttt aatggcactt aagtgtgttt ataaatgtga tttaaataat
      901 atagatacta gaaaatttag aggaatatgt gagtatgtag cacaggcatc aggaagaata
      961 cttcctacat ggaaaccagt agttggggat aatatgttta tacatgaatc tggtatacat
     1021 gcagatggag ctttaaaaga cccacataac tatgaaccat ttgacccaag tgaagtaaat
     1081 cttgaaagaa aaatagtaat aggtaaacat tcaggaagag ctgcagttgt aaataagttg
     1141 agtgaatatg aaatgtatat ttctccagaa aacgctacca agctattaaa tgctataaga
     1201 gctacatcta taagattaaa aagaagttta atggataaag aaattcttca attatattgt
     1261 gatatattag cacatgagaa aggcacaaca gaagaagaag cagttagagg ttcatatatt
     1321 taagatataa ttgtttagga ggaaaatatg ggagataata tagtttataa aataattaaa
     1381 aaacatatag tagatggaga agctgttgct ggaagttcaa ttggtataaa aatagaccaa
     1441 actttaacac aagattctac tggaacaatg acatatcttc aactagaagc tatgggaata
     1501 gataaagtaa agactaaaag aagtgtggca tttgtagacc ataatatgtt acaacaagga
     1561 tttgaaaatg cagacgacca taagtatata caaacagtgg cagataaata tggagtttat
     1621 ttctctaagc caggaaatgg tatttgtcat caagtatttt tagaaagatt ttcaacacca
     1681 ggagatacac ttttaggttc tgatagccac acacctactg ctggaggtgt gggcatgatg
     1741 gctattggag ctggtggatt agatgttgct ttagccatgg caggaggagc atactatata
     1801 aaagcaccaa aagtctgtaa ggtgaatcta gttggaaaat taaataatat ggtatcatca
     1861 aaagatataa tattagaagt gttaagaaaa caaactgtaa aaggtggagt aggtaaagtt
     1921 tatgaatatg gtggagaagg tgtcaaaagc ctatctgttc cacaaagagc tacaataaca
     1981 aatatggggg ctgaacttgg agcaactact tcaatcttcc caagtgatga aaaaacacta
     2041 gaatttttta agagtcaagg aagagaagac gcatgggtgg agctaaaacc agatgcagat
     2101 gcagtttatg atgaagagat tactataaac ttggatgaat taaaaccatt agctgcaaaa
     2161 cctcatagcc cagacaatgt agatgaagtt gaaaatatag gcaagattaa aatagaccaa
     2221 gtggcaatag gaagttgtac aaattcttct tatgaagact taatgaaagt agcacaaatc
     2281 ctgaaaggaa ataaagtaca taaggatgtg agtttggtaa tagcaccagg ttcaagacaa
     2341 gttatggaga tgattgctag aaatggggct ttggcagata taataagtgc tggagctaga
     2401 atactagaaa attcttgtgg tccatgtatt ggtatgggtc aatctccagg tactgactct
     2461 gtgtctttaa gaactttcaa tagaaatttc tatggtagaa gtgggacttt gtcagcacaa
     2521 gtatatttag taagtccaga agtggcagca gtaagtgcaa tcaagggagt gcttacagac
     2581 ccaagagaat ttgatattaa atttacaaat ttagatgtaa atgaattttt gatagatgat
     2641 agcatgataa ttaaacctgc tgatgtagga agtgatgtag aagtagttag aggacctaat
     2701 ataaaacctt tccctcttaa tacagagcta agtcagtcta taggtggaaa agttatatta
     2761 aagacagaag ataatataac tactgaccat ataatgccat caaatgctaa gttacttcct
     2821 tttagaagta atataccgta tcttgcaaac tattgtttca atactgtaga tacagaattt
     2881 cctcagagag caaaagacaa taatggtgga tttatagttg gtggagataa ttatggtcaa
     2941 ggttcaagta gagaacatgc agctttagca ccattatatc taggtgttaa aggggtaata
     3001 gttaagagct ttgcaagaat ccacaaggct aacttgataa atagtggtat aattccaatg
     3061 gagttttgtg atgagaaaga ttatgaaaat atatctcttt tagataatct ggagatacca
     3121 aatattttag acaatctagg tagtggaatt ttagaagtta agaatacaac taaaggaact
     3181 tcttttaagg tcaaagtaga attgtcagca aaagaagttg atgtattaaa agctggtgga
     3241 aaattaaact acactaaaaa tcaagctaat taatatgaaa agagggaata ttgaatgtat
     3301 aaagtgacac ttataccagg agatggaata ggaccagaag ttgcgaaagc tatgaaaaag
     3361 gtagtagaag ctactggagt agaaatagaa tgggaagaag ttaatgcagg agaggcagtt
     3421 attgaagagt atggtactcc acttcctgaa tatattatag atagtataaa aaagaacaaa
     3481 atagctataa aaggcccaat tactactcct gtaggtaaag gatttaggag tgtaaatgta
     3541 actttaagac aggcattaga tttgtatgta aatttaagac ctattaaaag ttttaagggg
     3601 ataaaatcaa gatatgaaga tgttgattta gtggttgtaa gagaaaatac agaagattta
     3661 tatgctggaa tagaacataa aataggtgat tatgcagctg aaagtataaa gataataaca
     3721 agaagtgcaa gtgaaagaat cgttgatttt gcatgtaact atgttaaaga taataagaga
     3781 aaaaaagtta cagctataca taaggcaaat ataatgaaaa tgtctgatgg attgttttta
     3841 gatgtgttta gagaagttgc atcaaaacat ggtgtagaat atgatgattt gattgtagat
     3901 gcagcagcta tgaacttagt tttaaatcca gaaaattatg atgtaatggt aatgccaaat
     3961 ctttatggag atatactatc agatttaggt gctgggcttg ttggaggttt aggtataatt
     4021 ccaagtgcaa atattggtaa agattgtgcg atatttgaag ctgttcatgg aagtgctcca
     4081 caaatagcag gtcaaaataa ggctaatcca acagcactta ttcaatcatc agtaatgatg
     4141 cttagatact taggtgaata tgaaaatgca caaaaaatag aaacagcttt agaaaaagta
     4201 tttcttgaag gaagtaaatt gactgtagat ttgggtggct cagcttctac tacagagttt
     4261 gcggatgagg tgtgtaagta tattgtatag attttattta gggaaaaagt aaaatataga
     4321 ttgaaggaaa gaggctttct caaaatagag ataagccttt tttattggaa tgataaaata
     4381 tatttgattt tatacaaaag agagttcctc ataaacaaaa aagttaattt tgggatatcc
     4441 attttaattt taaaattaat tgattaatac gcaaagaaag attaaataaa tttattattt
     4501 ttaattgaac tttcaaccaa aatatggtaa aatttaaaaa agttgaactt tcaactaaaa
     4561 atgaaaatat tgattatgtg aaattctata aaacagaaac tatttgactt tagccatggt
     4621 agtttcagtg gaggtgtgtt tgattaagac tttagatagt aatatattaa gagaagtagg
     4681 tactttatct agagcagtaa attcaataaa tgatattaag tacaaggaat taaagttaca
     4741 aaaaggacaa tttacatttt tgacaagaat ctgtgaaaat cctggtataa atcttgtgga
     4801 attatcaaat atgttaaagg tagacaaggc tacaacaacc aaggctatac aaaaattaat
     4861 aaaggcagga tatgtagata aaaagcagga taagtttgat aaaagaggat ataatttaac
     4921 tccaactgat aaatctttgg aagtttatga gttgataatt gaagaagaga atagaagtat
     4981 agagatatgt tttgataatt ttacagatga agaaaaacaa gtggtaacta agttattaga
     5041 aaaaatgagc aaaaacgtag aaaatgaatg gtttaaagta aagagatagt aaataaatac
     5101 attaacttaa ggagatattt ttatgattag aaaagcgaat atgaatgatt tagaaagcat
     5161 tatgaaaatt ataaaatcta cagtggaaga aatgaagact tataataata cacaatggga
     5221 tgaaaactat ccattagaaa aagactttgt ttcagatatt aagaaacaag atttatatat
     5281 ttatgaagtg gatggagaag tagcagggtt tatatgtctt aattatgaag agccagaaga
     5341 atatttaggc ttaaattggt catctaataa aaaagcaatg gtaatacata gaatggctgt
     5401 aaatccaaac tttagaaaga caggaatagc atctaagtta gttgattttg ctgaaaaatt
     5461 agctgtagaa aacaatgtat catacttaaa gagcgataca tattcaataa actcaaaaat
     5521 gaatttatta cttacaaagt gtggttttat aaaaatcgga gaaatgagtt ttttaggaaa
     5581 ggagaaatct ttctactgtt atgataaaat attataaaga gcatttataa tacaaggggt
     5641 aaataataga ttttcagcat tagtaagggc ataatataac ctagtatatg ctattatgaa
     5701 agggatgatt tatatgattg gatttctaaa gaaaagaaga agtattagaa aatacaagga
     5761 tgttgaagta gaaaaagaaa aattagataa aatattaaaa gctgctcttt tagctccatc
     5821 atcaaagggg cttagaacat gggaatttat agtggtagat gataaagaaa agctaattaa
     5881 tttatctcaa tgtagaacta aaggtggtgg atttttctta aaaaatgcac ctttagctat
     5941 tgtaattatt gcagataaag aaaaaaatga tgtttggata gaggatgcaa gtatagctgc
     6001 ttcatacata caacttcaag ctcatgaact aggtttaggt tcttgttgga tacaagtaag
     6061 aaatagaatg catgatgata atatagaagc tgataagtac attagagaag aattaaaagt
     6121 acctagcaag tacagtgtag aatgtataat ttctatagga tatcctgatg aagaaaagaa
     6181 agcatacggt gactctgatt tagactataa aaaggttcat tttaaccact tttagtatat
     6241 aatatgttgt attaagacat tatatttttt tctattgtta atatatactt aaaattgtaa
     6301 aaatatatct gaattttact aacttatcta ggggttatgt acaaggattt taaagataaa
     6361 tatagaaatt aatgtttata atcgatacat aacttgagta acaaaattta aggaggttaa
     6421 agtatgggta tatttaaaac aaaaatggat gaggactgga aagtaaacta cattaaagag
     6481 tttaatgaaa tgagagattc ttatgagagt aaacttcaaa aaaaacagtt tgaggtagat
     6541 agtttgaaat cagaattgga tagactaaga agctataaaa atagccttaa gccaaaagaa
     6601 aaacaaatta ctgatgatga tataaataat ataaagagtt taagaagaga tggcttaagc
     6661 tataaagaaa tttcaaatca aacaagttgg agcaaagcaa ctgtaagtag ggtattaaat
     6721 ggactttacg attagcatat acatggggtg atttaaattg ggatgaagta tagttacaaa
     6781 aaatgcataa tagattctat tttattgatg tttatagttc aaatattaag gatgattttg
     6841 aattatgtac ttctaagtca atttgaattt actcttgaaa attttaatat tataaactta
     6901 atttctttta cattagttgg tttatcgtta attttatttt taaaagataa ttctttgtat
     6961 aacaaggtta gaaatagaaa aatcacagaa gcttttgaag aaaataaaaa caatatactt
     7021 attgaaaagt gtaagctgat actttttgtt gtggttttat ctttagcaat aattgtaact
     7081 tattgtacta agggatatgt actttttaat gtaactatga tgactttgtc tgtattgata
     7141 gtcccaatat ttgaagagtt attttttaga gaatatatat ggaactatct aagtaatttt
     7201 ataaagtcaa agggtaaaat tatctgtata acatctatac tatctggaat ttataatata
     7261 ggatacatag atgttataag aaattatgta atattgtata acaattcaag ctacaccttt
     7321 gaagttatta tttcaaaaat tatgataggt actgtttttg gtattgtact tggattagtt
     7381 aagtataggt ttagagatgt agggttttgt atattattga ggagcttatt tgctatattt
     7441 attaggtaga tagtataatc ggtttataag gttgttttaa atttaatttt aaatcaacct
     7501 tattttattt gtg
//
LOCUS       DAELJO010000175         7468 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELJO010000175 DAELJO010000000
VERSION     DAELJO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.177"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..1293)
                     /locus_tag="KPB88_003135"
     CDS             complement(<1..1293)
                     /locus_tag="KPB88_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055376.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVFMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGAVCLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTG"
     gene            complement(1293..2039)
                     /locus_tag="KPB88_003136"
     CDS             complement(1293..2039)
                     /locus_tag="KPB88_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055377.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2186..4405)
                     /locus_tag="KPB88_003137"
     CDS             complement(2186..4405)
                     /locus_tag="KPB88_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0055378.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     SEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            complement(4771..6165)
                     /gene="gltA"
                     /locus_tag="KPB88_003138"
     CDS             complement(4771..6165)
                     /gene="gltA"
                     /locus_tag="KPB88_003138"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG0055379.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            complement(6165..7058)
                     /locus_tag="KPB88_003139"
     CDS             complement(6165..7058)
                     /locus_tag="KPB88_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG0055380.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
BASE COUNT         2471 a         1326 c          714 g         2957 t
ORIGIN      
        1 tcctgtagga aaaattattt tattttttga tttttttact tctatattta aattttttat
       61 actataattt tttaattgtt ttaaagaatt tatatgttct tgtgaattgc tactaggaat
      121 tataatccct tcatattttt ctaactttga actaatttta tctaatccca attcctttga
      181 taatttgtta taattcttta cacttagtaa gacttcccta ttatatttaa gtacaggaaa
      241 acttaactcc ttataatcat atcctttgat tgcttctcta atttgttgta gttcttcata
      301 ttctttttca ttattatgct ctgaaaaata tgagtaaacc attgggaaat tatttttaga
      361 agcttctcct tgagaactta caagtgttga agttgttgta aatgctacta aagtagccga
      421 taaaagtatt gttattaaaa ataacaatcg tgtattatcc tttaatttat atatcaaatt
      481 acttataagt aacatattta ctttgttctt ataaaatttt tctttatttt ttaaatactt
      541 taatatcaaa atagtaaatt gagaaaaaaa taaaaatatc cccaaagtaa ttaatagtgc
      601 tgttgtatac atacctccat ttatttttaa cttattgcca aatagtatca tagcatatcc
      661 taatataaga cacactgcac caattattcc aaatagcttt gaagatttta tctctttttt
      721 aggctttttt gtacctacta aaagctctaa aatactcttt ttactcaata attttaagct
      781 cattggtcct gttatcaaaa ataaaatcat aaatgataca atagtcaata gcattgcttt
      841 tactggaaaa taaaattcag ttaaattcat ataaaacagt ttgcttaaat acaataaaaa
      901 caattttgaa attgataaac caagtaatat tcctaacatc actgctaaaa atcctattat
      961 caaattttca attaatatca attttttcag ttgtctgtta ctcattccaa ttatcataaa
     1021 tactccaaaa tctttcaatc tatattttaa gaaatttcct aaagagtaca atataaaaag
     1081 tagactaaaa ccgaatatta ttaattctga agtcataaga gccataaatg cagtagaacc
     1141 actctgcatt ccagattttg ttataattgg atggaacata gacactgcaa aaacaaaaaa
     1201 tatcattata gaaaaaacag aacttaaaaa atatgctaaa tatgccctga aatttctaac
     1261 tatattatta taagcaaact ttctaaaatc cactatctta cacctcctat ttgagacaaa
     1321 acagctagta tatcagaata gaaagaatta ctgctttcag atttttttat ttctgtatat
     1381 atacaaccat cttttataaa aattactcta tctgaataac ttgcactata tgcttcatga
     1441 gttaccataa gtgttgttac attttgttct ttgtttatcc tagtaaataa tttcaaaaca
     1501 tcatcagtag attttgaatc aagatttcca gttggctcat ctgcaagaag tatggcaggt
     1561 ttatttataa tagctctagc aatagcacac ctttgagctt gacctcctga aatttcatag
     1621 gtttttctat ctaaaatttt atctattcct aaaaatttag atatactttt agtttgtata
     1681 ttcatatctt tgatacttgc cccctctagt gtaagtggca acataatatt ttcgccaaca
     1741 gttaaagtgt caatcaaatt aaaatcctga aatacaaaac caagttgttt ccttctaaat
     1801 tcagcaagtt tttctccttt caatttactt acatcatatc catctaatac aacctcacct
     1861 tcactctgtc tatcaactgt agatattata tttaaaagag tagatttacc acttccagat
     1921 ggtcccataa cagacacaaa ttctcccttt tctatattta gactaacctt atctaatgct
     1981 ttaaatggaa tatttccaca atatgttttt gatatatttc taatactcaa tatgttcatt
     2041 ttatattctc cataattttt tatttttact acaataccat aatatcgtga atatactact
     2101 ttaaccataa aaatacccta acaattaact tacatatttg taagtaagtt gttagggtat
     2161 cttatagtct aataattatt aatgattaat caaatttaaa gtttaagtgt ttttcaaatt
     2221 catctaatac cataggttta gcaaataagt atccttgaac cttatcacaa cctatctctt
     2281 tcaagaactc tacttgttca taagtttcta cgccctcaca gattacttta atatcaatcg
     2341 cttttgccat ttccacaatc ttagaaataa taatttttcc acgttttgta tctgttgttt
     2401 cgattaaaaa tcctctatct aattttaaaa tatcaaaaga aaggtctttt aacagattta
     2461 aagatgaata tcccgaacca aaatcatcca ttgatataag gaatcctatc tctttaatct
     2521 ttttcattat atcaatcaat atatttaaat tatcaaacac aacactttct gttagttcaa
     2581 gttcaataaa ctcaggagat atattgtatt tacttatcaa atcagttata ttttctataa
     2641 aattatccct atataaatga actctagaca tatttacaga gattggtaca ggagtatacc
     2701 ctttatttat ccacttgttt aatgttttgc atacttcttc aaacacgaac atatcaatat
     2761 ttacaataaa accattcttt tcaaataaag gtacaaaatc tataggtgaa ataaacccaa
     2821 attctggact attccatctt atcaaggctt ctgctccttc aatttcattt acatcatcta
     2881 aactatactt aggttgataa tatactataa attcaccttt ttctaaagct ttattcatat
     2941 tatcttccaa tcgtttttcc ttgtacaatc gatttcttgt atcttcatta aaaaatgaat
     3001 atgaatgttc atatttttcc tttttacttt ttgctgcaat atttgctcta tcaattaaca
     3061 agtcaatctt tttaatatca tcctttttag taatcttata tattccactt gacataacaa
     3121 ttttatactt agatgagtta gtagtattta agttacaaat ttctttattt attttagata
     3181 atctatttaa aagttcatct tcagtgttac aagcaatcaa caatataaaa tggtcattat
     3241 ccatcctagc acaaatttca tatttatttg tattattatt taaaacttgt gatatatgaa
     3301 ttaatatctt attcccttca gcatatccaa acatctcatt tatatattta aatttattga
     3361 catccaattg tactaagtaa aaattatctt gataataatt ctttaaaata tacatagaat
     3421 cttttctaaa cttttcttta ttttttattc cacttaaaga atcataataa gccaattttt
     3481 taagttgttc tattaattta gcattactta atttccacaa tatcataaat attactatat
     3541 aaaaatacaa ctttatcata aaattaacta ttagattttt aaagttctta aatatattac
     3601 attttgatac taaagtccaa ttagtccact ttatgttcgt atattttaca ttatataatc
     3661 tccaattatc ccaagataaa aaatcacctt gacatttttt atttatattt tttaagattt
     3721 cacttttagt ggttcctata atattccctt ctaaatcaag catcttactt ttatcattta
     3781 catatttttt gtttggatga gaaataatag tataatcttt atctatcatt agaaatttaa
     3841 tagaatcatc aaaattgctt ctacttatta aattatttac atcttgaaaa taaaaagaag
     3901 caaaaatggt cccaatcatc tcactatttt ttacaattgg tacatatatg ataaatactt
     3961 tgtcacctgt tacattactg acttcaatat ctgaaactac tacttgcttt gttttaattg
     4021 ctttctcaaa actaggctta tcttttattg agttaacatt ttccctatat gtacttgaag
     4081 catttccttt tgtatctgtt ataccaatca taaaaagatt atattccttt tgatatggac
     4141 ttaataattt tcccttttca attagtgatg tttcaccatt agaaaatcta tcatcttgac
     4201 taagagcttt taataaagat atgtttactt ctaaatagtt ttcaagttct ctttttgatg
     4261 cctcaatcat atttctagaa gtaatttcca tagagttact tgcatatatg atggtacttg
     4321 ttaaatcaat caaaactaat agtattaata agattaatat atgcttaaaa actagcccat
     4381 caaatacttt ttctcttttt ttcatattaa aataccatta attacacaat ttatttttct
     4441 attatttaat gttagatttg acaaatctga ttttattagc cacacattat tcactcctta
     4501 caagaccttt attgtataac tattttatca tagttagaca aggttatgtt acttagtatc
     4561 aaatattaca ataataactg ataaattatt aaaccatcta aaaaagtggc tacatcaaaa
     4621 tagagattgt actaagatct aaaagttaga ctttctacag gaatgaaatt ttcattgttg
     4681 aaatgattaa aacatatttg attttgtaca aaaagagtgc ctcatgaact aaaaagttca
     4741 ttttgagaca ccctctcttt aatttacaaa ttaagcatta acttttgctt ttaaatattc
     4801 atctattgat gctgctgctg ttttaccagc acccattgca gatattactg ttgctgctcc
     4861 agtaacagca tctccacctg caaatacacc ctctttagaa gtctgaccat tttcatctgt
     4921 tattaagcat ctcttcttat ttatatcaag actctttgtt gtagaagata ttagtggatt
     4981 tgggcttgtt ccaagtgaca ttataactgt gtctacatca agtacaaatt cagaaccttc
     5041 tatttctata ggtcttcttc ttccagaatc atctggctct cctaattcca ttcttataca
     5101 aaccatacct ttaacatatc cattttcatc tactaaaatt tccttaggat ttgttaatgt
     5161 attaaatata atgccttctt cttttgcatg atgaacttct tcagctcttg ctggaagctc
     5221 tttttcactt cttctataaa ctatatatga ttctgaacct aatcttaatg cagttctagc
     5281 agcgtccata gcaacatttc caccacctac tacagcaact tttttaccag aactaatagg
     5341 tgtatcataa tcatctctat aagccttcat taagtttact ctagttaaaa actcatttgc
     5401 agaaaatact ccatttgcat tttcccctgg tatattcata aacattggaa gaccagctcc
     5461 tgaacctatg aatatagctt caaatccttc atcttctata agttcatcta ctgtaattgt
     5521 tttaccaaca attacattag tttctatttt tacacctaat tttcttatat tgtctatttc
     5581 aggttgaaca acagcttgtt ttggaagtct aaactcaggt attccgtaag ttaaaactcc
     5641 acctggctca tgcattgctt caaagattgt tacatcatag ccttttttag ctaaatcacc
     5701 agcacaagct agacctgctg gaccacttcc tattactgct actttttgat ttttcttagg
     5761 ctctgtatca gataaattta tgtcattttc ttttgaccaa tctgctacaa atctttctaa
     5821 tttacctatt gatacagcat ctgattttat tccaagtata catacacctt cacattgact
     5881 ttcttgtggg catactctac cacaaacagc tggtaatgaa ctgctcttag ctattacttt
     5941 agcagcacct tctatatcat cttctttaat ttttgttata aaccctggta tgtcgattcc
     6001 tactggacat ccacccacac attttggttt tttacaagct aaacatctat tagcttctgc
     6061 catagcttct tctttattat accctaagca tacctcatca aaatttgttg ctctcactgc
     6121 aggttcttgt tctctaactg gtacttttac ttttttagca tccattattt gttacctcca
     6181 catccacaat tactatgatg atgactatcg ccttcttcaa gttttagcat agctcttccc
     6241 tcttgagtct tgtacataga ctgtcttctc attgcttgat caaaatcaac taaatgacca
     6301 tcaaattcag gaccatctac acatgcaaac ttagtttcat ttccaacact aactctacaa
     6361 ccaccgcaca tacctgttcc atctatcatt attgtattta aacttactat agtaggtata
     6421 tttagttctt tagttaattg gcacataaat ttcatcatta tcattggtcc aattactatt
     6481 gcttggtcgt attttttacc ttgattatca actaaatctt ttaataggtc tgtaactctt
     6541 ccattaaatc catatgtacc atcatctgtt gatacatata catttttagc tacttttttc
     6601 atatcatctt ctaatataat taattcttta gttcttgcac caattattac atctacatct
     6661 aatccatgct ctttaaacca tttaacttgt ggataaactg gagcagaacc aactccacca
     6721 gctacaaata atatcttttt atttcttaat tcttctatat cttcatgtat aaattcactt
     6781 gcttgtccta atggccctac aaaatccatt acaaaatcgt tttcttcaaa cattgctaac
     6841 tttttagttg aagcaccaac tgtttggaat acaatagtta ctgttccttt ttctctatca
     6901 tagtcagcta ttgtaagagg aatcctctct cctttttcat catttttaat tattataaat
     6961 tgacctggtt gagaagattt tgcaactcta ggtgcctcta tttccattaa ataaatacta
     7021 tctgttaatt gtctcttact aactatttta ttactcatct cgctattctc cattctaaca
     7081 aatatatttt tcccacttta aatttaatta aaaacataaa cacacaaaac tataactctt
     7141 tataatataa tattagttct tggttgattg ttttgtattg gacatccccc ctataataat
     7201 tataactgcc caaactacaa atagttgata ttaacaaatt tcttgtaaat gccaataaac
     7261 actaagtcta tatcatacgt ctaatttgtt atagatttaa cctaatacac acttaaagta
     7321 tactatatta tgccaaattt gtaacaaaaa ttttataaat acataataaa atttttttat
     7381 tcaattattt atttaataaa ttaacttagg ttttcataat aacactaatt tctttaaaaa
     7441 ctaaacgtgt ataaggcttt aatctatt
//
LOCUS       DAELJO010000176         7432 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELJO010000176 DAELJO010000000
VERSION     DAELJO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.178"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            51..1028
                     /locus_tag="KPB88_003140"
     CDS             51..1028
                     /locus_tag="KPB88_003140"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG0055381.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1021..1989
                     /locus_tag="KPB88_003141"
     CDS             1021..1989
                     /locus_tag="KPB88_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG0055382.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2173..2763
                     /locus_tag="KPB88_003142"
     CDS             2173..2763
                     /locus_tag="KPB88_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0055383.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2782..3552
                     /locus_tag="KPB88_003143"
     CDS             2782..3552
                     /locus_tag="KPB88_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG0055384.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3689..4657
                     /gene="dusB"
                     /locus_tag="KPB88_003144"
     CDS             3689..4657
                     /gene="dusB"
                     /locus_tag="KPB88_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG0055385.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4766..5245
                     /gene="greA"
                     /locus_tag="KPB88_003145"
     CDS             4766..5245
                     /gene="greA"
                     /locus_tag="KPB88_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG0055386.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5260..6789
                     /gene="lysS"
                     /locus_tag="KPB88_003146"
     CDS             5260..6789
                     /gene="lysS"
                     /locus_tag="KPB88_003146"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG0055387.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7098..7292
                     /locus_tag="KPB88_003147"
     CDS             7098..7292
                     /locus_tag="KPB88_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055388.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2960 a          754 c         1505 g         2213 t
ORIGIN      
        1 tttattagaa gaaaataagt catgaaatta tgtcatatta gaggtgaaat atgcgagata
       61 aaataataga gattatatta aataataaat ctgaatttat ttcaggagaa gaattatcaa
      121 aacaattagg ggtgtcacgt gcagctatat ggaaacatat gaaagcctta aaagaagagg
      181 gatataatat agaatctgta aataaaaaag ggtatcgatt agcggaaaat ccaactgatt
      241 tattaagccc acagaatatt tactataaat tagatacaga gtttattgga aaaaatattg
      301 aacatcttga cactatagat tcaacaaatg aacatgctaa aaggatagct ataaatagtg
      361 tagatgggac tgtgatttta agtgaacatc aagacatggg gaaaggacga ttgggaagaa
      421 gatgggattc aaaacctcat gaaggaatct ggatgagtat tatattaaaa ccaaatatgg
      481 aaccattcaa agctccattt ataactttaa tagcaggagc aagtgtaacg aaagctttaa
      541 acaatctagg agcaaataca ttaataaaat ggccaaatga tgttatttta aatggaaaaa
      601 aaatatgtgg tatattaaca gagttatctg ctgaaattga aagaacaaat tatgttgttc
      661 ttggaattgg aataaatgtt aaaataatgg attttgaaca agaaatagct gacaaagcca
      721 catcattgta taaagaaggg tatatgttat ccagagtaga tattgttagg aatgtattga
      781 ctgaatttga aaaattatat ttagattata ctgtaaataa ttccaaagaa aaaaccttaa
      841 aaatatgtag agattattct gcaattatag ataaaaatat atatgtatta aaaggagata
      901 atagagaact agtgaaatgt ttagacataa atgaagaggg aaatttatta gttcaaagaa
      961 gtgatggaag tataaaagaa attatttcag gtgaagtttc tataagagga gaaaaaggat
     1021 atgtataata atattttgga tatagaaggc ttaaaggttg ggcaggttga ggatagaaat
     1081 ggtcttactg ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg tgttgatgta
     1141 aggggctctg ctccagggac tagagaaacc gatttattag accctgtaaa tatggttcaa
     1201 aaagttcatg cagtagtttt ggctggaggt tctgcatatg gtcttgaatc cacttgtggt
     1261 gtaagtagat acttagaaga aaaaggaatt ggctttgatg taggtgtagg taaagtgcct
     1321 atagttgtag gagcagtttt atttgattta ggagtaggga gctttaaatg tagaccagac
     1381 ttaaagatgg gttatagagc ttgtgaagtt gcaaatactg atattttaaa tcaaggaaat
     1441 tatggagcag gttgtggagc cactgttgga aagataagag gtcaggaata tgctatgaaa
     1501 ggcggaatag gtagctattc tataaaatta gataatggtc taattgtatc tgcattaatt
     1561 gctgtaaatg ctcttggaga tgtttatgaa aatggtaagg ttatagctgg tgctttaaat
     1621 gataaaaaga cagaaacaat tgatacatat aaattaatga aacaaggtga aagtaaaggt
     1681 ggatttagtg tagataatac tacaattggg ataattgcaa caaatgctaa gttaaccaaa
     1741 gctggatgta aaaagatatc tcaaatggga catgatggat atgctaagtc tatttttcct
     1801 atccatacac ctcatgatgg agatacaata ttcacaatat caacaggaga aatagaagca
     1861 gatataacat tagtaggttc attggctgta gaggttgtgg aaaaaagtat aataaatgca
     1921 gtaaaaaaag cggatagtgt agaaaatata atggcatata aagatatagt aaaaaaacga
     1981 gaaatatgat gaaattagca tatttttctt gtcaaattgt tgacaatggc aactcatctt
     2041 gataaaatta ttcttgttag agcgggcatc ctacaatggt agcttgactc atagttataa
     2101 gaaaaaaacg aaaaaaatta aaaaattaag tagtccggca tcttaatgag ctggaacgga
     2161 ggtgtatttt ttatgatgaa cacaagtgca tcagtaagaa aagtaaatgt aagaaagatg
     2221 actataatag gagtactatc agctatttct attatgctat ctatgactcc attgggattt
     2281 attccagttg gacctacaaa agcaactata atgcacatac cagttattat tggagctatt
     2341 atggaaggac caatagttgg ggcagcaata ggatttatat ttgggatatc aagtctctta
     2401 aatgctataa caaacccaac agtaacatct tttgttttta taaatccatt agtttcaata
     2461 ttaccaagag ttatgatagg tgtattagct tattatgtat atcagttaat aattaaggct
     2521 acaaataagg tatacatatc aggtttaatt acaggggcaa taggttctct gttaaataca
     2581 gctggagttt taggaatgat atatgtctta tatgctgaca agtatcttca tgcaatgggt
     2641 aaaagtggtt cagcaggaaa agtaataatg gctcttgccg cagcaaatgg ggtgccagaa
     2701 gcaatagtag gggctttagt agtagctgct gttgccactg tacttaagaa aagcaaaaaa
     2761 tagaaagaag gttaatgaca tatgcttcta gtatttgatg ttggaaatac taatatggtt
     2821 ttaggtatat ataaaggtga caaattagtt aattactgga gaattaaaac agatagagaa
     2881 aaaacgtctg atgaatatgg aatcctaata agtaacctat ttgattatga taatgtgaat
     2941 ataagtgata ttgatgatgt tataatatca tctgtagttc cgaatgttat gcattctctt
     3001 gaaaactttt gtataaagta ctgtaaaaaa cagccattaa tagtaggtcc aggcataaaa
     3061 acaggtctaa atataaaata tgataatcca aaacaagttg gggcagatag aatagttaat
     3121 gctgtagcag ggatagaaaa gtatggagca ccaagtatac ttgttgattt tggaacagca
     3181 actacatttt gtgctatctc tgaaaaaggt gaatatttgg gtggaacaat agcaccagga
     3241 ataaaaatat ctagtgaggc gttatttcaa agtgcgtcta aattacctag agtagaatta
     3301 gctaagccag gtatgactat ttgtaagagt actgtatcag ccatgcaatc tggaataatt
     3361 tatggatatg ttggtttagt tgacaaaata ataagtatga tgaagaaaga attgaattgt
     3421 gatgatgtta aggttatagc tacaggtgga ttagctaaac tgattgcttc agagacgaaa
     3481 agtatagatt atgtagatgg ttttttaaca ctagaaggat tgagaataat atatgaaaaa
     3541 aaccaagaat aattaatata cagggaattg ggttatatat ccaattccct ttttatgata
     3601 gatttctagc tatatgtggt atattataat gaggaaataa gaatttaaag ttaaatcaca
     3661 aaataaaggc aaaaatagga gaaaaacaat gaaaatagga aatttagaat tgaaaaataa
     3721 agtttttcta tcacctatgg caggtgtaac agaccttcca tttagattaa tttgtaaaga
     3781 acagggatgt gggcttttat atacagaaat gataaatggt aaggcacttt gctatgatga
     3841 tgaaaatact aaaaaaatgc ttaagataga agaggaagaa catcctgtag cagttcagat
     3901 atttggttca gaaccagagt ttatgggaag agcagcagag ataatgaatg attattcaaa
     3961 tgagattctt gatataaata tggggtgtcc agctccaaaa gtagttaaaa atggagatgg
     4021 ttcagcactt atgaaaaatc ctaagcttgc agaagaggta ttgagggcag tagtgaagaa
     4081 ctcaaaaaag ccagtgactt taaagattcg aaaaggctgg gatgataata gtgtaaatgc
     4141 tgttgaaata gcaaagatag cagaggattg tggaataagt gctttagcaa tacatggaag
     4201 aactagagaa cagttttata caggcaaagc agactgggat ataattgcag agattaagaa
     4261 aaatttgagt atacctgtta taggaaatgg tgatgtattt acaatagaag attcaataaa
     4321 tatgttagac aaaactggtt gtgatgctat aatgataggt aggggtgctc aaggtaaccc
     4381 ttggatattt aaaagaataa accattatat gaatacaggc gagattttgc cagagccaac
     4441 tcttaacgaa aaaataagta ctgccattaa gcatttaaaa cttgcagtag aagaacatgg
     4501 agagtatgta gctgtaaggg aaatgagaaa acacatcgct tggtatttaa aaggacttag
     4561 aaactctgca aaattaaggg atgaaattaa caaaatagaa gactatcaag aagttgtgtc
     4621 gaaattggag tactatatgg aagactcctt gacataaaaa taatagtgac ttataatata
     4681 gcgcataaat taaaaaaaga ttataaaatt tttaaaatat aattaggaaa attcctaaga
     4741 aatttgtgaa ggagttgtat ttataatgga agaaaataaa gagtttttat tgacacaaga
     4801 gggatatgat aaattagaag aagaattaga aaacttaaag gttgtaaaga gaaaagaagt
     4861 tgcagagaga ataaaagttg ctatatcttt tggtgattta tctgaaaatg ctgagtatga
     4921 tgaagctaaa aaggaacaag cacaagttga agaaagaatt ttaaaattag aaaatatggt
     4981 tagaaaagct gttatcatag atgaaagcaa aatagacctt aatgttgtta caataggatc
     5041 aatagttaaa gttaaggatt tagaatttga tgaagaggtt gaatatacta tagttggttc
     5101 gacagaagca gacccatatg atggtaaaat atcaaacgaa tctcctgtag ggaaagcact
     5161 tttaggtaga gctgcgaaag aagttgtgga agtgcaagtt cctgatggag ttgctaagtt
     5221 tgaaatatta gaaataagaa gataatttgg aggaaaaaaa tgaaaaataa tcaacaaagc
     5281 aatgaagaag cgcaaattca agaagacctt agtgaagtac ttcaagtaag aagagataaa
     5341 ctaaaaaaat tacaagaatc aggtagagat ccttttaaag aaagtagata tgatagaact
     5401 cattattcta tggatataaa agataacttt gattctcttg agggtaaaac tacaaaaata
     5461 gcaggacgta taatgagcaa gagaatacaa gggaaagctg gctttataga tattcaagac
     5521 caagagggaa gaatccaatc ttatgtaaga ctagatgcta taggagagga agaatatagt
     5581 gtattttcaa catatgatat tggagatata gttggaattg aaggagaaat ctttaaaact
     5641 aaaaaaggtg aaatatctgt aaaggctaaa tctgtagtat tactatgtaa atctttacaa
     5701 gttttaccag aaaaatatca tggattaaaa gaccaagaac taagatatag acaaagatat
     5761 gttgacttaa tagtaaatcc agaagtaaaa aatgcttttt taattagaac aaaagcatta
     5821 aaagcattaa gagcatactt agatgataga ggatttttag aagttgagac tccaatatta
     5881 aatactatag caggtggggc aaatgctaga ccatttataa ctaatcataa tactttacat
     5941 ataccaatgt atttaagaat agcgaatgaa ttgtatttaa aaagacttat tgttggtgga
     6001 tttgataaag tttatgaaat gggaagaatg tttagaaatg aaggtatgga tttaaaacat
     6061 aatcctgaat atactgcaat agaattatat caagcatatg cagactatac tgatatgatg
     6121 gaaataacag aaaatgttat tgcacatatg gctgaagttg ctacaggaag tatgatagtt
     6181 aactatcaag gaacagaaat aaactttaca ccaccttgga aaagaatgag tatggaagat
     6241 tgtgttaaag aatattctgg tgtagatttt tctactataa atacagatga agaggcacta
     6301 gaagttgcta gagaaaaagg tatagagata aaacctggaa tgagaagagg agaagtaata
     6361 aatgctttct ttgaagaatt tggagaagat aaattaatcc aaccaacatt tataactcat
     6421 catccagttg aagtatctcc attatctaag agaaatgttg aagacccaag aagaactgat
     6481 agatttgagg catttgctaa taagtgggag ttagctaatg cattttctga acttaatgac
     6541 cctattgacc aaaaaggaag atttatagac caattaagaa aaagagaatt aggtgatgat
     6601 gaagcttttg aaatggatga agatttctta aaagcattag aggtaggtct acctcctaca
     6661 ggaggactag gaataggtat agatagagta attatgttat taactaactc tccatctata
     6721 agagatgtat tattattccc gactatgaaa cctattgata acaatccaaa taaagaagaa
     6781 gaaaactaag aaatattagc attctactaa gttttaaata aagtttagct attaaattat
     6841 caataaatag ctaaatttta tttaaaatgt cagtgtagaa tgctttttat atgcttcaaa
     6901 gattctttta gatatttaat ttataaatta ccaacaaacg ttagaaattg atattagttt
     6961 ttgttgattg agactttata tttagaaaat atgattgaaa gtaatttatt atgaaaatat
     7021 ttagaataat ttatgagaag gtaaaaagaa taatttatga gaatactata tataaaaaat
     7081 tattggggga atgtctgatg tttcaagtaa ttaatttttt atgtttattt tgtgttggat
     7141 ttttggttct tgatgttatg atatattttt gtaaggatgc aatggatatt tttgagggta
     7201 caactaatgt taataaagtt aagagtataa atgtttgggc agatagagtg agagctaatt
     7261 catattctta tgaaaataaa attgcaaaat aggtaaaatt atatatgcat gcattgtttg
     7321 taatatcaag ggttatatct attagtttta attatattta ttaaaaaaaa ccaaaagtta
     7381 taaccttttt taaaataaaa aacaaaaaaa gtattgacga attattttta ag
//
LOCUS       DAELJO010000177         7383 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELJO010000177 DAELJO010000000
VERSION     DAELJO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.179"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            125..634
                     /locus_tag="KPB88_003148"
     CDS             125..634
                     /locus_tag="KPB88_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG0055389.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIKAIVK
                     ELKDNGILS"
     gene            646..1527
                     /locus_tag="KPB88_003149"
     CDS             646..1527
                     /locus_tag="KPB88_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0055390.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1655..1860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1986..3257
                     /gene="serS"
                     /locus_tag="KPB88_003150"
     CDS             1986..3257
                     /gene="serS"
                     /locus_tag="KPB88_003150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0055391.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3578..4033
                     /locus_tag="KPB88_003151"
     CDS             3578..4033
                     /locus_tag="KPB88_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG0055392.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4034..4125
                     /locus_tag="KPB88_003152"
     tRNA            4034..4125
                     /locus_tag="KPB88_003152"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4068..4070,aa:Ser,seq:gga)
     gene            4163..4427
                     /gene="ffs"
                     /locus_tag="KPB88_003153"
     ncRNA           4163..4427
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPB88_003153"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4504..6141
                     /gene="dnaX"
                     /locus_tag="KPB88_003154"
     CDS             4504..6141
                     /gene="dnaX"
                     /locus_tag="KPB88_003154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG0055393.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6200..6550
                     /locus_tag="KPB88_003155"
     CDS             6200..6550
                     /locus_tag="KPB88_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG0055394.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6563..7162
                     /gene="recR"
                     /locus_tag="KPB88_003156"
     CDS             6563..7162
                     /gene="recR"
                     /locus_tag="KPB88_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG0055395.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKKEIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
BASE COUNT         2991 a          747 c         1372 g         2273 t
ORIGIN      
        1 tcttttttta tttattataa aagttaagat taaatagaat ataaagtcaa tattgattta
       61 taataagata aaatataaaa ttatatagta taggtaaagt ttttatttgg aaagaaggtg
      121 taatatgaat tctaatgaaa gaagaagtaa attaatagat attttaaaag aatctaagca
      181 tcctgtaaaa ggtggaacac tagctgaact attaaatgta agtaggcaag taatagttca
      241 agatatagcc cttataagag ctagaggatt tgaaataata gctacacctc aaggatatat
      301 aatatataat caaccgtatt ttacaaaaca aataaagtgt aaaaatcata aggattctaa
      361 agaaatttat gatgaattaa agataattgt ggatttaggt ggtattataa aagatgtcat
      421 cgtaaatcat ccaacatatg gtcagataac tgcagaactt aatatttatt ctaaaatgga
      481 tatagacaac tttatgaaga aagttgagac aaatgaattt aaacaattat cggttctgac
      541 agaatgtagc catattcata caattgaggc aataaaagaa gaaactataa aagctatagt
      601 aaaagagtta aaggacaacg gaatattatc ataaggagga gttgaatgaa ttttttatca
      661 ttggctaaga ctgcctcaac aaatagcaat ataactaaga gaactttaga aaatatgctc
      721 gataatttta gtgataatat accaaatatt atatatgcaa taattgtatt tgtgataggt
      781 atttatatat ctaaaatagt aagaaggatg gtttcaaagt ttctaatcaa atatggaatg
      841 agtaaaggtg taaataattt tattgtatat ggaatatata tatctatgtt atctattata
      901 tcattgataa gcttaggtat tatagggata caaactacat cagttgtagc tgtgttagga
      961 gctgctggat ttagtatagg tttagctttt aaagaaatat tatcaaattt aggctctggt
     1021 atgattattt tattttttaa gccatttaat ataggtgatt atattcaagg ttcaggtgta
     1081 gaaggtacag tttctgacat acaaatattt agcactgttc ttaagacacc agataataaa
     1141 actataatta tacctaattt tcaactgaca agtaataata taattaatta cacacatcaa
     1201 aacaaaagaa gaatagattt ttcatataat atttcatatg atagtgatat agatgtagtt
     1261 aagtctatat taaatgaaat ttttacaaac gaaaaaagaa tattaaatga tccaaagcct
     1321 ataataggtt taaactctat aggaaataac accatgcaaa tagttgctag accttgggta
     1381 aaaactgatg attactggga tgtatatttt gatgtaatgg aaaaggtaaa aaataaattt
     1441 gatgagaatg atataaaagt tccatttgtt ccttcttcat tattatttaa taatacaggt
     1501 aatctaaata caatggataa aagataaggt cttaattaaa tagaaaaaat gaatttaata
     1561 taaaaaatta aagaaaattc taaaaaaatt tgaataatag tattgaaaga tgttaatata
     1621 tataatataa tgattatcaa ataaaacaat tagttaaaaa gctgtgaagg aaaatagtaa
     1681 aaaacagatt gtattcagag aatgagtgga tggtgaaaac tcattacaat ggtttttgaa
     1741 tctactctag agcttctagt taaagctaga cgtttaccta cgttataggt ctgagaggat
     1801 tatacatttt agtatagtca attagggtgg caacgcggat aaaagatttt cgtccctttt
     1861 atagggtaaa cggaggtctt tttttatttt taaaatttgt ttaaactaag agtttacaag
     1921 tataatgttt gtaaattaag ttggaattat aacttatatt atcaaaatta ttaggaggaa
     1981 ttaatatgtt agatattaaa agaataagag aaaatctaga tgatatcaaa aaagctatgg
     2041 aaagaagagg tgaaagagaa tttgaccttg atgctgtggt ggaattagat aataaaagaa
     2101 gagaaatact tcaagaagtt gaagttatga aaaatgagtt aaatgtaaat tctaaaaaaa
     2161 tacctcaatt aataaaagaa ggaaaagatg taacagaaga aaaggcaaga ttgaaagagt
     2221 tatcagataa gataaaaggt attgatgaga aagtaaaaga agtagaagca aaaatggaat
     2281 atacattaat gagaatacca aatgttcctc atccagaggt accacaaggt gaaacagatg
     2341 aagacaatgt tcaaataaga acttggggag aacctactaa atttgatttt gagcataaag
     2401 cgcattggga tataggtaca ggtctaggta ttttagattt tgaaagagca gggaaaataa
     2461 ctgggtcaag gtttacttta tataggggat taggagctag attagaaaga tctttaatga
     2521 atttcttttt aaatactcat acagctaagc atggctatac ggaagtctta cctccattta
     2581 tggccaatag aaatagtttt atagggactg gtcaacttcc taaatttgaa gaagatatgt
     2641 ttaaaataga aggattagag tatttcttaa taccaacagc tgaagttcct gttactaata
     2701 tacatgctaa tgaaatatta gatgttgcag agcttccaat taaatactgt gcatatactc
     2761 cttgttttag atctgaagca ggttcagcag gaagagatac aagaggttta gttagacaac
     2821 atcaatttaa taaagttgaa ttagttaaat ttgttaaacc agaagattct tataatgaat
     2881 tagaatcatt aactcatgat gctgaaacta tgttacaaat gttaggtctt ccatatagag
     2941 ttgtaaaaat atgtacagga gatttgggat ttacagctgc atttaaatat gatttagaag
     3001 tttggatgcc aagctacaat agatatgtag aaatatcttc ttgttcaaat tttgaagatt
     3061 tccaagcaag aagagcagga ataagattta aaagggataa aaaatcaaaa gcagaatatg
     3121 ttcatacatt aaatggttct ggattagcga taggaagatg tttggctgct atattagaaa
     3181 attatcagca ggcagatggt tctgtagtag ttccagaaga attaagacct tatatgggtg
     3241 ttgatgttat aaaataaatt tatattaatt gtatatgtag agtatgcttt gatgaagaga
     3301 atatattatg agtaaacaat aaataaaaaa actatgaaat tattataaat attaatttca
     3361 tagttttttt atgttaattt acacagcaga attttcatca taaaacacta aatattttta
     3421 taaaacactc ttgtatgaat atctaaaaat tattttagaa ctaatttaaa aaaaatatag
     3481 ctatatatag taataattaa ataaacaaaa taaaattaag ttgaattata aaatgaatta
     3541 tgataagata agtttgttat aagttttggg aggaattatg gaaagttcat tttatatgaa
     3601 agaagcatta aaagaagcat ataaagcata taataaaaaa gaaacaccaa taggtgctat
     3661 tatagttaaa gataatcaaa taatagctag agctcataac cttacagaaa cgttaaaaga
     3721 ctctacggca catgcagaaa ttttagcgat aaaacaagca tcagaaaaat taggtggttg
     3781 gaggttgact gattgtgatt tatatgttac aatggaacct tgcataatgt gtagtggtgc
     3841 aatagttaat tctagaataa aaaagcttat aatagggaca agacatgtaa aaaattcata
     3901 tatagaaaaa cagcatgagt ttaaattaga ttattttaat aataataatg taaaagtagc
     3961 ttttgatgtt ttgcaagaag aatgctcgat catattgcaa gaatttttta aggcattgag
     4021 aaaaagagat taaggagaga tggtcgagtg gtcgaaggcg ctcgcctgga aagcgagtat
     4081 atgggtaaaa ctgtatcgtg ggttcaaatc ccactctctc cgccagaaac aataaaagta
     4141 attaaatatg gttgtaattt tgctgtacta gatggggagg tagcggtgcc ctgtaacctg
     4201 caatccgcta tagcaggatt gaattccatc taaaggctta ttttctgtag ggccgcccta
     4261 aataagtggt gttgatgctt aggtcctgcg caatgaaagc tcatgaaccc cgtcagatcc
     4321 ggaaggaagc agcggtaagt gatcactttt gtgtgccgtg ggggtgccta agttgagcta
     4381 actgttttgg taacgcctat ggaattaagt cgatagatgg tgtacagcat ttaatatata
     4441 aaagactaat cccttagata atgagggatt tttttattaa ttttgctaaa gagaggtaaa
     4501 aagatgcata aagcattata tagagcatat agaccacaaa aatttgaaga tgttattggt
     4561 caagatcata ttattaagac tttaaagaat caaatatata gtgataatat aggacatgct
     4621 tatttgtttt gtggaacaag aggtactggt aaaacatcta ctgcaaagat tttttcaaga
     4681 gcagtaaact gtttgaataa aataaatgaa gaaccgtgta atgagtgtga gatatgcgaa
     4741 tctgtattaa aagataatac aatggatgtt gtagaaatag atgcagcttc aaataatagt
     4801 gtagatgata taagagagct tagagaaagt gtgaaatatt ctccagccaa tgctaaatat
     4861 aaagtatata taatagatga agttcatatg ttgtctcaag gagcttttaa tgcattatta
     4921 aagaccttag aggaacctcc atcatatgta atttttatat tagcaactac agagcctcac
     4981 aaaatacctg caaccatttt atcaagatgc caaagatacg attttaaaag agttacagtt
     5041 aaagatatga cccttagaat gaaaaagatt tgtgaagatg aaggaattga tatagatgat
     5101 aaagctctta atttaatagc tagaaattct caaggagctc ttagagatgc acttagtata
     5161 ttagaccaat gtatgtcttt tggagaaagt aagatagatt ataaagatgt tgttgaatta
     5221 atgggaagcg taaatataga acaattattt gaattgtctc aatgtattgt agagcaggac
     5281 actaaaaagt ctcttgagat attaaatgaa tttgtcttat ggggcaaaga tataagaaat
     5341 cttataaatg acttaattga tcattttaga aatttaatgg tatgtaaggt ttcatcagaa
     5401 ttggatgaaa taatttcatt accagaagaa actatagaac aacttaaaat tcagtctaaa
     5461 aatatagata taaatgattt aataaggatt ttaaatatat tatcaataac tcaggatgat
     5521 ataaagtcat cttcaaatcc aagggtttta gtggaaataa ccataatgaa aattgctcaa
     5581 ccaatgtttg atgaaagtaa agaagctctt ataaaaagag ttgagaactt agaaaaaatg
     5641 attgaactgg ggaactttaa gtctgaaaaa attgggaata ataaagaaaa agagtatgag
     5701 gtcgatagag aaatagatgt aaaacaagaa aatgtagttt atgaagatgt aaaaaatgag
     5761 gacgttatac ttatagagtc atcttggaag aatatactta aacaaattaa aaaagataaa
     5821 aaaatgccaa tatacgcact cttaagtgaa gtaaaaagtt ttaatgtata ttctaatatg
     5881 ttgtatgtta tatttgatga taaatttgat tttgcaaaaa ctagactgag ctctcaagat
     5941 acaataaatt atttagaaaa aacaataaga gatgttttaa atagaagttt taatgtaaaa
     6001 atagtactga catcagaagt taaagatata aatcttgaag ttaaagagaa aaaggacatt
     6061 ggtgaggaaa tattaaagaa catagtgagt gaagagatat tagaaataaa agatagtatt
     6121 gatgaaaatg agagtaaata ataaaatact attatgatat aataataaaa tgaaaataag
     6181 tttaggagga atagtgttta tggctaaaaa aggttttgga ggcggaatga tgcctggtgg
     6241 tggaaatatg aacaacttgt taaagcaggc tcaaaaaatg caagaaaata tgcaaaaagc
     6301 tcaacaagag ttagaaagta aagaagtaga agcttcagtt ggtggaggag cagttactgt
     6361 aaaagttaat ggtaaaaaag aagttataga tataactatc aagccagaag tagttgatcc
     6421 agatgatata gaaatgctac aagatttagt tttaagtgca gtaaatcaag cattgagaaa
     6481 tattgatgat atacaagcaa gtcaaatgag taaggtaact ggaggtatga atatacctgg
     6541 gttattctag taggtggtat ttatgcaagt ttatacaggt cccataacga gacttataga
     6601 ggaattttca aagcttcctg gagtaggaag aaagactgct caaagattgg cttttcatat
     6661 tattaatatg aatactaatg atgtggaagc tttatctaaa gcgattatag atgcaaaaaa
     6721 agaaataaga tattgctcaa tttgttgtaa tataacagat acagacccat gtagcatgtg
     6781 ttcaaataaa agtagagatt caagtgttat atgtgttgtg gaagatccaa gagatgtagc
     6841 agctatggag agaacgagag agtttaaagg acagtatcat gtattaaatg gagtaatttc
     6901 tccaatggat ggaattggac cagatatgtt aaaaataaag gaacttatcc aaagacttgg
     6961 caatcaggaa gtcaaagaaa taataatggc aacaaatcct actattgaag gtgaagctac
     7021 agccatgtat attgctaggt tagtaaaacc aatgggaatc aaagtcactc ggatagctca
     7081 tggtcttcct gttggaggcg atttagagta tgcagatgag gtcacaatat caaaagcact
     7141 agaaggcaga agagaaatat aaaaatcgag gttaatacct cgatttttgt agttttttga
     7201 gttaaattca aattaatttt gatttagatt tattgaataa atttcttctg tattgtataa
     7261 tttgttgtat aaatcattta aaaattttga taggggtgca acatgaaacc aattatgaac
     7321 ttagaagaac ataatgcaat gaaagaaaaa agagttaaaa aaatgaaatc aaaagataaa
     7381 cct
//
LOCUS       DAELJO010000178         7332 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELJO010000178 DAELJO010000000
VERSION     DAELJO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.180"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            160..1461
                     /locus_tag="KPB88_003157"
     CDS             160..1461
                     /locus_tag="KPB88_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0055396.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1537..1812
                     /locus_tag="KPB88_003158"
     CDS             1537..1812
                     /locus_tag="KPB88_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0055397.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGIYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            2926..3156
                     /locus_tag="KPB88_003159"
     CDS             2926..3156
                     /locus_tag="KPB88_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055398.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3149..3730
                     /locus_tag="KPB88_003160"
     CDS             3149..3730
                     /locus_tag="KPB88_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055399.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4309..4986)
                     /locus_tag="KPB88_003161"
     CDS             complement(4309..4986)
                     /locus_tag="KPB88_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0055400.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5175..6539)
                     /locus_tag="KPB88_003162"
     CDS             complement(5175..6539)
                     /locus_tag="KPB88_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0055401.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6667..7188
                     /locus_tag="KPB88_003163"
     CDS             6667..7188
                     /locus_tag="KPB88_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0055402.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2844 a          841 c         1267 g         2380 t
ORIGIN      
        1 atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa taatacagtc
       61 ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta tttaaaaatc
      121 aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc aaaagatgtt
      181 gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag ctataagcta
      241 gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt taaaattatg
      301 aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc aaagatagat
      361 agaaaatctc tagcaataaa aacgagtgat gtgactaatc taattacttg gggtcagaat
      421 tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag aagcataaat
      481 caaaactgcc aaaacctttt acacagccta aaccttggag aaaatatcga tacttgtatt
      541 tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga aaatgatgat
      601 gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga tattggattt
      661 aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg agagctaacc
      721 acaatagcag ctaaatcggg ggttgggaag actgctttgg cacttcaaat aatgttaaat
      781 tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag tgagcaagta
      841 tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa atctaaagaa
      901 atagatgaaa atgattggaa acttattgta aatgcgatag gtgatttaag cgaaaataac
      961 ttaatttata ttaatgataa gatagataca attagtgcaa ttagaaagag gataaggcag
     1021 gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca gaaaagcatg
     1081 gataagagag aaagggaggt agctactttt tcgagagaac ttaaaaatat gactttagat
     1141 tttaatatac ctgtaattca attaagtcag ttaaatgatg aaatgaaaga ttcaagacct
     1201 tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa caatgtggtt
     1261 tatatacatc aattaaaagg gagtgattat gaagaagcag tgagagatat tggagaaagt
     1321 gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt aatagttgcg
     1381 aagtgtagag atggacagac taggcataag catttttgtt actttgggga taagttacat
     1441 tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt aaaagtcggg
     1501 aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag aaaaattata
     1561 gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca taagtatagt
     1621 ttattggaat accttgaatt gtgtcaacaa agtgggggaa tatattttta tattaaaaca
     1681 ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt taatgggaaa
     1741 aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag gcatattgtt
     1801 agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa tagaagttat
     1861 tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt aagattatag
     1921 aggagtataa gtgtgattaa caacatgatt ttgtgaataa aattgaggat atatatgatt
     1981 taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta aaaggagggg
     2041 ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat ttaatagatt
     2101 aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa aatcttgata
     2161 tgtgattaat gatgcaatat gtgaataaag ttaagattat aatagattta acctgtggct
     2221 aatagaataa aattgtgaat aaagttaaga ttataatagg tttaacctgt gattaataga
     2281 gtaagagtgt aaatagagat aaacttcaat atgagcttaa cctatgatta atagagtaag
     2341 agtgtaaata gagataaact tcaatatgag cttaacctat gattaataga gtaagagtgt
     2401 aaatagagat aaacttcaat atgggtttaa cttgtggtta atagagtaag gtgtaactaa
     2461 agataaactt caatatgagc ttaacctatg attaatagag taagaatgta aatagagata
     2521 aacttcaata tgggtttaac ctgtggttaa tagagtaggg tgtaactaaa gataaacttc
     2581 aatatgagtt taacttgtgg ctaatagaat aagattgtga ataaagttat agttttagat
     2641 gggtattaac ctgttactaa tagaatatga ttaggtgtaa aattatagag ctgcataaga
     2701 aaatattata gatatattaa atattagata ataaatattt atatagatac acaaaagaaa
     2761 aggcgatatg aagctaaact taatgacttc atatcgtttt ttatttgaaa ctcctttgta
     2821 tttagagtgt gatttttaat attgaggttt tatgtgatta agtcacagat aaaaagtaag
     2881 tatcttgata taattcttat agattaataa tatagggagg ttattatgtc taataaaaaa
     2941 aagaagtatg caaatatttt agcaaatgag tgcgttgctt gtggtagctg tataaaagca
     3001 tgtccaagaa gtgctatctc agttccatgt ggcattagtg caaaaattaa tagagattta
     3061 tgtgttggat gtgggatttg tgaaaagata tgtccagcat ctgtaattga gattataaca
     3121 atattaaaag aggaagaagg taagtgtcat gaataataaa aaacattggt atgactattt
     3181 atggattttt tcttcaatat atttgatatt gggatttgta aatattattt tcgcttggat
     3241 tgggctaatt tgtttctttg ttccactagc tatttctatt gttaagggaa ataaagctta
     3301 ctgtaataag tattgtggaa gagggcaact gtttaatttg cttggaaata aattgaagct
     3361 gtcaagaaat agagatattc ctaaatttat tagaagtaag tggtttagat atgggttttt
     3421 gacatttttt atggtaatgt ttgctaatat gcttttttca acttatttag tgtttgaagg
     3481 aagtagaaat tttaaagaag tagtgacttt gttgtggact ataaaaatgc cttggcaatg
     3541 ggcgtatcat ggaactttag tttcaccttg ggtggtacag tttgcatttg gtttttatag
     3601 tgttatgctt acatcaactg ttcttggttt gattactatg atattgttta aacctcgttc
     3661 atggtgtgtt tattgcccta tgggtactat gactcagatg atatgtaagg caaagagtaa
     3721 gtctgtatag aaaggcctta tgtgatattt acagttttgt tcaagtagag atgagtagta
     3781 ggtttgaata tatatgacaa atatatgagt ataggggcat agatttagtt tgtgtaaggt
     3841 aaactgattt atgcttcttt tttatgtctt agattattgg ctaagtggaa acaatgaatt
     3901 tagatattaa gaattattca gttatttgaa gaaaatagaa taataattaa cattatatta
     3961 atggatattc attacatata agccagttaa tagcaagatt tatttaatat gaaataggtc
     4021 aagtatttaa actaaataag gtcatgacaa ttaatataag tatagatata aaaagattag
     4081 aagttataac ttataaaagg tattctatat aaatagtatg gggaaaatag tcaaattaac
     4141 ttattagaaa caaaaaatat acataaaaaa taaatgtaaa tatcaaataa gatactatat
     4201 caaaatagat ataagtatct tttttattat tgaaattatg aaaatatatt tgatttcaca
     4261 caaaaaagag tgcctcatga actaaaaagt tcattttgag acacactttt acagtgtcaa
     4321 agaacgaagt ttatccttat ccaatacttt tataccacca cgagataaag acacaatacc
     4381 ttctctagca aaatatttca acattctcga tacaacttct ctggcactac ccatatactt
     4441 agcaatttgc tcatgagtca tatttatagt gctactgcca tttttagcta tctcatcaat
     4501 taaaaaagat gccaaacgct tatcaaaact cataaaaaga atttgttgca tagcccacat
     4561 aacatcagaa aaacgttcgg tagctaattt atatgaaaat aactctgcat gaatattttc
     4621 tgtagataat tttgcaaaaa cagaggaact aatctgtata atatcgcagt ccgtttcagc
     4681 atctacatag acatcaaaag ttattgtttt tagaatacag gaagctgata aaatacatac
     4741 atctccatca tcaagtcgga acagtgtaac ctctcgacct tcatctgaaa gtatgtatgt
     4801 acgcactgtt ccagacttta taatcaaaat gccaatacag tcactatcac cacaatgtac
     4861 tggattccct tttttgtaat gtgaggtatt tgctgaagaa attaataatt ctttttgtaa
     4921 gtcagtaagt ttatcccaaa aaggcaagct atgtgtaagt atatcgattt cattttgtga
     4981 aatcatacaa tatctccttt cttaaattgt tataatccca tgatacaagt gaatagataa
     5041 attgacaagt agtttgttaa taggattatt tagtcagata gtaatagtat gatttatgta
     5101 gaaaaaatat gataatatgt attcttgcta ttaatgagaa agtaatatgt tttgatgata
     5161 gtaggtttgt ataactaatg cataccgttt atttttaaaa gattaagttc cttatgtaat
     5221 cttatagaca taaaaccagc aattatagca gccatcaaat cagctattgg ttgtgcatat
     5281 aagataccat ttaacccata gaatacagga ataatcaaaa ttatagggat aaaacagaca
     5341 ccttgtctac acatacctaa aaatccacct tcctttgctt ttcctagtgc gaggaataat
     5401 gtagaataca ctgtatagaa tccaaatagc ataaatgaaa acccatttgc cctaagagct
     5461 ttactaccat tttctattaa taaaatgtca ccacttgtaa attgtgaaat tatagatttt
     5521 gggaagagta tcaatataag accaagcact acacaaaata ctgtagtcca taatatggaa
     5581 gtttttattg ctatatgtaa tctatcgtag ttctttgaac caaaattata accagcaatt
     5641 ggttggaaac cttttataaa accaaataca acaagactac ctaaagacgt tattcgtgtt
     5701 acagcaccca ttccagctat cacagaatca ccatactttg tagcctccat attagttaat
     5761 gtaatggcaa gactagtcaa taattgaaat attaaggtag gtatgccaat ttttaggatt
     5821 tctgaaaaaa tttcttttga aaaacagcat tcgttaattc taaaactaaa tatacttttc
     5881 ttgcttaaaa tatatcctac ataaacaagt gttgaagcaa tctgtgagat agctgttgca
     5941 atagaagcac cccttacccc cagaccaaaa gtataaatga atatcgggtc taaaatcata
     6001 ttaagcaaag cacctaataa catagcacac atagttgttt tagcagctcc ttcacttgtg
     6061 acaatattag tcatagtgac attgaatata ttaaaaatag atgatacgac ataaatgcta
     6121 gcataagata ttgcataagg cataatactt tttgtagctc ctaataattt taaaattgga
     6181 ttgagaaaaa tcataatcac aataataact aatgtaccca ctataatact actgtaaata
     6241 gatgtactta ccacttttcc tgctttttca ttatcaccat ggcctaaaag tctggcaaca
     6301 tatgaagcag ctccatttcc aaacaataat cctaatccca caacaagctg acctaatgga
     6361 aatgcaacgg atatagctcc catttgactt gttccaagtc cacccacaaa gtatgcatca
     6421 accaaattgt ataatgcagt tatcatcata cctatcatgg taggtaatcc catagataac
     6481 aatgcttttg agattggggc agaccctaat aattcaattt ttttgttact ttctttcata
     6541 cttaacactc ctcttctatt gacattaagt ctaaattata atactataaa ttatagtaag
     6601 tatggaaaat agtactataa tttatagtat ttgtcaatag taagaaaact aaaggagatg
     6661 atttttatgg ctacaataga tttaattgta ttgggaatag taaagcaaca acccaagagt
     6721 gcttatgata ttcaaaaggc agtggaatac aggaatatat cacgttgggt taaaataagt
     6781 acaccatcaa tttataaaaa agtaattcaa ttagaacaaa agggatatat tgaaagtaaa
     6841 actgtgaaga atggaaaaat gccagaaaaa gcagtttatt cattaactga attaggtgag
     6901 acacattttt tagatttaat gtttgataca tctagcaaac caataaatat tcttttagat
     6961 tttaatgctg ttatagtaaa tttaaatagc cttacaccag aaaaacagaa attatgtctc
     7021 aataatattg aagagaatgt tcaagagtta aagaaaacta ttgaggaaaa tataagtata
     7081 aagacagaag taccagagac agcaaaagca gtgttggagc aacagttggt gctagcacaa
     7141 gcattggaaa cctggataaa ttctttaaaa gataatatgg gaaattagaa ttagagaagc
     7201 taacttaata atcattaaac aaaaaccagc atatttaata atgagatatt agaaaaaatg
     7261 aaacaaatga tataaactga aaagtatatt taaaataaag ctcacttaat tgagggagaa
     7321 tgttaaactt tt
//
LOCUS       DAELJO010000179         7318 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELJO010000179 DAELJO010000000
VERSION     DAELJO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.181"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            512..1204
                     /locus_tag="KPB88_003164"
     CDS             512..1204
                     /locus_tag="KPB88_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055403.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1400..3460
                     /locus_tag="KPB88_003165"
     CDS             1400..3460
                     /locus_tag="KPB88_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055404.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3666..3989)
                     /locus_tag="KPB88_003166"
     CDS             complement(3666..3989)
                     /locus_tag="KPB88_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG0055405.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3982..4683)
                     /locus_tag="KPB88_003167"
     CDS             complement(3982..4683)
                     /locus_tag="KPB88_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG0055406.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4961..6346)
                     /locus_tag="KPB88_003168"
     CDS             complement(4961..6346)
                     /locus_tag="KPB88_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0055407.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSSSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6454..6597)
                     /locus_tag="KPB88_003169"
     CDS             complement(6454..6597)
                     /locus_tag="KPB88_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055408.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(6792..7214)
                     /locus_tag="KPB88_003170"
     CDS             complement(6792..7214)
                     /locus_tag="KPB88_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG0055409.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         2821 a          830 c         1028 g         2639 t
ORIGIN      
        1 aaataagttt gtattttagt tatataaatt ttaatagaat atgtacagta tatagtagat
       61 agaatgtata aaaatagaat ttaaataagt agctattatt gcagtgaaat gaatgtttct
      121 aagtatattt gggtggaaga atggtggaag aaaattagtg aaatgtaatt cttccaccat
      181 tcttccaccc aaaaaataaa aactgttcaa taagaattaa tatacatcaa ttcagctgaa
      241 tgcaataaaa atacttaaat gaaagtaatc tgaaaacaac ataattcaaa tcaatttatt
      301 caaataaacc tcaatttatc ttaaatctta agaaatattt aagaattagg ataatatttt
      361 cttaagattt atattttatt atatagttat aagggaaagt tacaaaaatg taagccaaag
      421 taatataaca atttagtcat tgttgagaaa gggagtgaaa gatattgtat aatatagata
      481 agtattatta tacaatagag gaagtgaaaa tatgtacaat atattagtag tagacgatga
      541 taaagaaatt gttgattcga tagaaatata tcttaaatca gaaggattta aaatatataa
      601 agcatatgat ggaatgaaag ctttggaaat aataacagag aatgacattc atctaatact
      661 aatggatata atgatgccta aattagatgg aatcaaagca actgtaaaaa tacgagaaga
      721 aagaaatatt ccaataattt taatttcagc aaaaagtgaa gatacagata aaataatagg
      781 attaaatatt ggagctgatg attatataac aaagccattt aatctattag agcttatagc
      841 tagggttaaa tcaaacttaa gacgatatgt aactttaggt aattatgaag atacatgtaa
      901 agaaattcta aaaagtggag ctttggagtt aaatacacta acaaaagaag ttaaaattga
      961 tggtgaaaac ataaaaatga ctccaattga gtataaaata attgaacttc ttttagaaaa
     1021 taaaggacga gttttctcaa tagatgagat atacgaaaaa gtatggaatg aggaaagttt
     1081 taattcagaa aatacagtag cagtacatat cagacgaata agagaaaaga ttgaaataaa
     1141 tccaaaagaa ccaagatttt taaaggtggt gtggggagtt ggttataaga ttgaaaaatt
     1201 ttagtggaaa aattataaaa gaaaagagtt taatatttaa ttgcattttg attttagtca
     1261 tttttacatt tacacaaatg ttaggaattt taatatgttt taattttttc tctgagagtt
     1321 taatttactc tatggggatt atgcttttct taaactttat tttatataaa atactgacaa
     1381 tgtagatgag gtgggcaact tggagaaata tagagtatta aagttagttt cattaatcat
     1441 aatatctatc tcatttccat taactatatt gttggtgaca ataggacttg ctgagcctaa
     1501 atacaatata caaaaaactt ctacaaagag tgggattttt gatatatctg atgattttta
     1561 tgatagtaat atatttgacg aaggaacaat ggcaagtcta ttacccaagt ttaagctatc
     1621 tgtaggtggt aaattaacta gtaaagaaaa aggtaaattc caacaagaag gtaaaagtat
     1681 agatttaagt agtagtgatt ctgagtatga taaaatcatg attaatgaac aagcttctga
     1741 agaatattta agttatctaa aaaatataaa agtgtatgtt attaatacat acacaggagt
     1801 attttataca aatacgtcat atgctagttt atatgatttt aaacaaaaaa ctaagagcta
     1861 ttgtaatgta gagataataa ataagatagg aaagagctca tatattaaaa caataaatgg
     1921 agaaaaattt gaactgaata atctgagtct ggaactaaat gaagtagatg aaggttttga
     1981 ggcatatgta tcttttccag aagagccaac tatagaagat ggaatagtat atactaattt
     2041 tcaaatattt aaacaagcta cagaaaaagt aagattattt tttgtagcta gtttaagttt
     2101 agcaattata tgtttattat ctataatttt atatatcaaa ataaaaggtg aagttttaaa
     2161 aagaaataat gtatttttac taatatacag taagatacct atggaattta atgtaatatt
     2221 agtatctatt tcaattgcaa ttttaactat atatgctctt gctgtatctt atcatattga
     2281 tgtaattgca ttagtttctt tatctttact gatagtatca aataatatat tttttataaa
     2341 tgtgcaattg agttatttag aaaataaaat gtgtatattt aagacaagta ttatagtaag
     2401 agtgatttta tggttaataa aaactgtaag ggaggtcagt aacgctataa agaaagtatc
     2461 tttagcaagg aaagttattg ttatagtgat aatatcttta gctttaatca tggtaattag
     2521 cactcgaagt ataggtatgg gattactgtt ttctatatgt atttttttag gatttacaat
     2581 atatataact aaaagacttt catatcttag ttacgtaata gaaggtacag aaagaataaa
     2641 aaagggagaa ttagattata agataaaaat tactgggaat gataatttta cgtctttagc
     2701 tgaaaatata aataatattg gagaaggtct tgatagagct atagaagagc aagtaaaaag
     2761 tgaaagaatg aagtctgacc ttataacaaa tgtatctcat gatttgaaaa ctccacttac
     2821 ttctataata aattatgtag atttaattaa aaaagaagaa tcaatacaac cagagtatat
     2881 aaatgattat ataaatgtac ttgagtcaaa gtcaaaaaga ctaaaattat tgatagaaga
     2941 tttatttgaa gcgagtagag taagtagtgg caatatagaa ttacaaatta gcaagattga
     3001 tttagtgcaa ctattaagac aaagtatagg tgaacttgaa gaaaaattat ctaaaaataa
     3061 tttatattta aaattaaatg ttccaaatga taaggtgtat atatgggcag atggaagaag
     3121 aatgtataga gtatttgaaa atcttttatc taatatagca aaatattcct tagaagaaac
     3181 tagagtctat attgatgtat atgactatgg tgaaaatgta aaggtaacta tgaaaaatat
     3241 atcttcttat gaattaaatt ttgacccaag tgagattatg gaaagattta aaagagctga
     3301 tgaatcaagg aatactgaag gaagtggatt agggcttgct attgcaagag atttagtagc
     3361 tttacaaggt ggaaaatttt atatagaaat agatggagat ttatttaaag caaatttaga
     3421 gtttcaaaaa tatgaagaag aaataacaaa tatggaatga gatagtattt aagtatatat
     3481 atttttttta ctgtcgaaag cttttattat aatatattag aatatttaca gtagagaatg
     3541 aagagtaaaa acagagtaac tttaagtcac tctgtttttt atttaaaatc aataatataa
     3601 caacttaaat taaaattgta cacatagttt taaaataact tatacaataa aacattatcc
     3661 aaatatcaca agataattcc aaataaatat atagatatca ctcctgtaat aatagaaata
     3721 ccaatagatt taaacttata tgcaacaaga agtacaatca taaaagatat aattttaggg
     3781 ttagctatag ataaatatag actatctcca ttaaaaaata catctttagc agttaaggaa
     3841 gtaagtattg ctacaggtat aaatttcatg atttttttca aaaaatcagg catctctttt
     3901 tttgaaaaaa ttaacatagg aagcactctt ggtagatatg taacaatgta cattccaatt
     3961 ataataaaaa atatataatt attattcata agattcctcc tttttacagt aagattcttc
     4021 tataaagaaa ccaattaaag atgctaaaat tgatgcaact attattacta aattgctttt
     4081 gaataatgca tataaaataa cagaaataat gattgatgat ataaatacta caatataaat
     4141 cttatttttt atttgagaaa ctattaaagc tataaacata gaagtcaata tgaagccaga
     4201 aatagaactg tctatagata tactttctcc taaaaatgct ccaataaaat tagctaaaac
     4261 tgatgaaatt aagcagaaga aattaagata aatagcattt ctatcagacc aatttccagc
     4321 agtaaatttt tcataattaa ctgcaaaagt ttcatctgtt atagtatgac aaaatggcaa
     4381 tataaatttt ggacttttat tttttatgta catgttcaaa ttagaactca ttagaaaatg
     4441 tcttaagtta actataaatg tagtcaaaac aatagatgtt acacttgcac cagtagaaat
     4501 cattgaagct gcaatgaatt gagaacttcc tccaaatact aaagttacca ttagtgctat
     4561 ttgaaaaggt gaaagaccag atttttgtaa tagtattcct cctactatac ctataggtat
     4621 ataacctaag aatataggaa ttgaagcaat aaagctttct tttaattgtt gagttctttc
     4681 cacaataaat cactcctttt gtattaaatg tattaaagta ttcgtaaaac agaaaaatag
     4741 attaattgta taattgcaat tataattaaa aataaatttg gattaatata taaaatacta
     4801 tacaacaaat agataatctt aaacggttat taaattttat ctatttgtta ttatgatgta
     4861 ataatacata ttaatctcta taagttaatt actatctggt gtaattttat tttaacaaca
     4921 aaaaaaaata cacacaatta aaatattgtg tgcaatacaa ttattttttc tcttctaata
     4981 gtctttttaa tacgctcaaa ccagctttaa gttcttctgt actttctgta gcacatacag
     5041 ctattcttat tgcagaaatt ggtttttctt ttccaacagc aaaacgttca gaagcataga
     5101 cctgtacacc ttctttaagt gcttgtatct caaatttttc tgccattatt ccatcaggta
     5161 gaatcaacca tctaaatata cattcttcat ttcccaaaag attatatcca gataaatatt
     5221 gattaataat cttgtttctt cttcttgcag atttacggcg tgcattaatt aatttatcgg
     5281 cttcacctga tgtaatcatg cgataagcaa tttctgttaa aaaataagat tgagatagat
     5341 taatgttgta taatgcgctt gataatgcct ctcgatactg tttaggtgta gagatatatg
     5401 ctagccgtaa actaggtgca ataatttttg atgaactcgt aatataaatt gtttgattgg
     5461 gcagatagga agccactgga tttaaatgtg tttcattaag taaactatga atagcatcct
     5521 caataacaat taaattgtat ttagatgcaa tatttgcaat catttttcgt ccattttgtg
     5581 acattatatg tgtagttgga ttttgataat ctggtattat gtatagacct ttaatatttt
     5641 catttttaca agcatataat agtccttctt cactaatctc attatcttct tgtttaattg
     5701 gaattagttg aatcccaagc attttagctg ctgtctttat tccaggataa gttaatgggt
     5761 ctacacctat tctatctcca tgttgaaaca aaccagctaa aatagcaaca attgcatttt
     5821 gtccaccact tgcaggaagt aagctatctg atgtagtttc aagcccagct ttataaatta
     5881 atttggcaat agcttctttt tgccatttag caccatctct ttgtccatat tggaatatag
     5941 ttttaaaatc tatttcctta attatgtgtt taaacaaagt attcatttct tctagcgtga
     6001 aatctggatt cattgtgccc atttcaataa atgtaatttt attattactg gacatcaaga
     6061 atagatttga acgtgtatca taagatacaa aagttccact tcctgtaact ccacttatta
     6121 gacctttttt ttcacaaatt ttaaaagctc tagagatagt actcacatta acatctaaga
     6181 aatctgccaa ttcacgttgg ggtggtaatt tagtacctgg taataaaaat ccattgttga
     6241 tatcttgttc tagttgttct gcaagagcct tatataatat ttgacctttt gaaggtctct
     6301 ttggtttcca gttcatagga taattttcaa aagaattaat tggcataagt tttcacctca
     6361 cattattaat ttgatttttt ctttatctaa aacaataaat tctaaagata caaataaaaa
     6421 ctgataggaa ataattctat cagtttttgt cttttaattg atttcaaatc ttcctataat
     6481 ttttatacct tctttatcat aaagagggat tgttttttta aatatattaa aatttctttt
     6541 tcttgtatat aaaacagctt cttctggtgt ctttggttga ttatcacaat cgtacaaatt
     6601 tctttttttt aactaaaagc aataatacat aaaattatta ttgctaatga tactcttaat
     6661 gtggtatata gtcatagtgt cacatgtcta tcaatagttt tttcagtaat tattgtaacg
     6721 aatagtagga ctcataaaat acttagaaat atgtttttag ataaatttta aatatataat
     6781 ctaacttata tctataaatt aacactatta ttctccaatt ccatttctag tttttttata
     6841 aatttattaa attcttctgg ttgccttttt ccaaacaata ctcttacaag agctgaattg
     6901 atttttccaa atatattcct cttgctctca ctttcaataa aaataagttt tgtattttct
     6961 aagtcaatat gtactaattc atatctagta atagtacttt ctcttttatt gcttgttttt
     7021 atctcatata ctctattttt ttcgaaatta gttatttcaa ccttaaatct tttattattt
     7081 ttttgctgta caaatgttcc tactgctttt ttctcattaa aattaggaaa atttagtctt
     7141 gcagattttg taaatatatt aaatatgtct tttgcagaat attttaataa aacttcagtt
     7201 ttaaatgtat tcatatcgtt ttcccttctg tatataaatt taatatttat aagttaattt
     7261 ttaagttaaa atagtataat atgccatatt aaatcatgtt aaaactattt atatagct
//
LOCUS       DAELJO010000180         7304 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELJO010000180 DAELJO010000000
VERSION     DAELJO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.182"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..680
                     /locus_tag="KPB88_003171"
     CDS             <1..680
                     /locus_tag="KPB88_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055410.1"
                     /translation="NNSQKISSEDFSKLFNKNSIDTSIINNNVKQSSYEYFALVSLGF
                     LVIIFIMNNILSNYISESKGLSKRLYSMPITRVQFLIYDFVGLWIYSFIFLLLYVLFF
                     RILGITFKGNFAILLLLCALSSYFMTSISTFVTSFFSKKYGTAIVYALLFLQTIFGGI
                     FSMISDTFTKLTSLSPTYLIGELFSNYETFKTIDSIGNLIFTCLIISTILIALAIVKE
                     KYKWREV"
     gene            683..1747
                     /locus_tag="KPB88_003172"
     CDS             683..1747
                     /locus_tag="KPB88_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055411.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPAKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            1991..2488
                     /locus_tag="KPB88_003173"
     CDS             1991..2488
                     /locus_tag="KPB88_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0055412.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(2664..2912)
                     /locus_tag="KPB88_003174"
     CDS             complement(2664..2912)
                     /locus_tag="KPB88_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055413.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(2978..3994)
                     /locus_tag="KPB88_003175"
     CDS             complement(2978..3994)
                     /locus_tag="KPB88_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0055414.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(4052..4819)
                     /locus_tag="KPB88_003176"
     CDS             complement(4052..4819)
                     /locus_tag="KPB88_003176"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG0055415.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(4887..7076)
                     /locus_tag="KPB88_003177"
     CDS             complement(4887..7076)
                     /locus_tag="KPB88_003177"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG0055416.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         2377 a         1245 c          755 g         2927 t
ORIGIN      
        1 taaataattc tcagaaaata agttcagaag atttttctaa attatttaat aaaaattcta
       61 ttgatacttc tataataaat aacaatgtta aacagagctc ttatgaatat tttgctttag
      121 tttcactagg atttcttgta ataattttta taatgaacaa tattttatca aattatataa
      181 gtgaatcaaa gggactttca aaaagacttt attctatgcc tataactaga gtacaattct
      241 taatttatga ctttgtaggt ttatggattt attcatttat attcttatta ttatatgttt
      301 tattctttag aatacttgga ataacattta aaggtaattt tgccatattg ctcctacttt
      361 gtgctttatc atcttatttt atgacttcaa tatctacctt tgtaaccagc ttttttagta
      421 aaaaatacgg tacagcaata gtttatgcat tactcttttt acagacaatt tttggaggta
      481 tcttcagtat gataagtgat acatttacta agcttacaag tttatctcct acctatttaa
      541 taggagaatt gtttagcaat tatgaaacat ttaaaaccat agattcaata ggaaatttaa
      601 ttttcacatg tttaattatc tctacaatat taattgcact tgctattgta aaggaaaaat
      661 ataaatggag ggaggtttaa taatgattag attaactatt gcaaatataa aaagatatct
      721 aaaaaatcat actcttttaa ttaatatggt tatgcttcct attatactaa ttttttcact
      781 taattttttt atcaacaact cgggaaatca atcaatgtac ttttctccag tagcaattgt
      841 atctgactct tcaggaaagt atgaacataa gttaataaat agttctaaat taaaagaaaa
      901 ttcatttaga ttaaatgaac aagataaggc tatgaatcta ttaaaaaata ataaggtttc
      961 tgctgtattc gttttagata aaaatttttc aaatagtata gataaattac aacgacctgt
     1021 agttaaatgc tttaaaattg aaaatggtgg tggtagtctt tgggcagaat cacaaataga
     1081 atcttttatt actaaatcgt taaaattgaa agtggataaa aatatagatg ataaattgac
     1141 aaagacaaat ataattgata acaaaccagc taagaataaa ggttcatttt tagtagtctt
     1201 cttgatatgc tattttatgt atattaatgc ggcacatctt gcttctgatt tatttacatt
     1261 aaaaaaatca aatgtgctaa aaagacttat ctctacagat aataaagata taaaaataat
     1321 tttcagtata tttttaggac tattttttat tcaatcaatt gtatacactt ttgctctatt
     1381 atgtttttca attatagaag actttaattt gagtctaaat gtattgatga ttgtactagc
     1441 aaatagtttt gtttccactg gatttgtatt ttgggtagct agagtattta aaaatgaaag
     1501 ttctataagt cttatatcta cattttattc tttaataggt ctagctatat ctatctctag
     1561 tctaatacca tcaatggata aattatcctt tatgactaat ctttcaaagt tcacaccatt
     1621 ttattggact atagatgcta ttaaaaacaa tggtagtatt ttaataaata taatagctct
     1681 aatattaatt ggaattatat ttgtaactgc aggaagtctt aaactaagag attttgctaa
     1741 aaactaataa ttattaaata taattagtat ctatttttat ataattgtaa aagaatattt
     1801 caacatatta aattatttta tcttatattt ttctctttag tttcacacat caccaagtct
     1861 aaactttata ttatatatta atatttttga cttgtcaagt tatttatgtt ataatattaa
     1921 tcattagata attaaagcat tgtacctaag ttgcaatatc taaaattcaa tttggaggta
     1981 taactagcat atgtataaaa ttggtgaaag tgtaatgtat ccaaaagagg gagcctgctc
     2041 tgtaaatgat attgtaacaa agaaaataaa tcatgagatg caaaaatact atgaattaag
     2101 tgtcattttt aatagtaacc ttaaaatttc gattccagtt ctaaatgcag atagaattgg
     2161 catacgtcca gttatggatg gaaatgatgt tgataatttt atccaatcta ttaataaaac
     2221 agatggtgta tggatttttg atagaaaaga aagacttaaa ctgtaccaag ataaatttca
     2281 ttcaggagat gtttttgaaa ttgtaaaatt aatcaaaatg cttatgatac aagacagttc
     2341 aaaacaattg tgtagcacag ataaggagtt tttaaataaa gctcaaaaat ttgctttatc
     2401 agaattagct gctgctcaat gtaaatccta tacaatggta ttagaggaaa tgaaaaaaca
     2461 tatattaaat tcaaagaata taaattaaac cagtttttta aactggtttt attttttata
     2521 ctaatcatcc atgctacttt agcttatttt aaactgccta atctaatatt aataaagtta
     2581 tctcaattag atagatgtat tttattcaaa taaaaaaaac taagagtaaa tcatattact
     2641 gatttactct tagctaataa aatttaatct tcttcaagtt cttttttctc ttgtaataat
     2701 acacttaatt catttgcttc taaaacttgt gcctctgcaa tatcaagtaa ctcttgttgc
     2761 cattccacac atttatcttt gtaatcaaaa ggtagcgttc tgtcaactat gtacttatca
     2821 atacagtcct ccgcactttt aattatttct tttctttcat caataaattt ttctaattct
     2881 actaaggctt cttcataagc ttttctactc atataaattc cccctttgta caattattct
     2941 acattaaaat gatttttcct cttttttgta taaaatttta tttgtatata acttttccat
     3001 caatatcttt tatagtttga catccagtta atatcataga actacttagc tctgattgaa
     3061 gtttatttac ataagtttct acaccatcag ttgcaccacc aaaagaagca gtcacaaaag
     3121 gtcttcctat taatactgca tctgcaccta aacctatcat ttttagtacg tctaaaccag
     3181 ttctcactcc accatctgca agtattgtaa ctttaccttt aacagcatca gcaatttttg
     3241 gtaaaacttc acatgctcct ggtgtacagt caagcactct tcctccatga tttgatacaa
     3301 ctattgcatc cacacctgct tctactgcca ttaaagcatc ttcaacagtc attatacctt
     3361 tcaatataaa tggtagttta gtagacttta ctaactcctt tatttgttta acatttttag
     3421 gaagtactgg ttttccatgt aatgataaag ttactagacc acaggcatct atatcaactc
     3481 caactgcaaa tgcaccagct tcttcagcta atcttatctt ttttataata ttttcattat
     3541 cccaaggttt aataaataca attccagctc cattatactt tttgactaca tctaaatttt
     3601 ccattaaaaa agcatctact gccgtatcac caaccattgc ataaatacca ctattagcac
     3661 atcctgcaac tactggttct atgtaatctc tttcattaat ttttccaccc atatttaaag
     3721 tagttcctgt tactggagct gcaaatatag gtgcactcat tttctttcca aacatctcta
     3781 tagatgtatc aggatttgat acatcatgta ttactctcat atttactttt actttttcta
     3841 aactcttgct attttctata aaagaagaac cacttccttt acctcccatg ccaggcactt
     3901 cacctgcaca agcaacccca ttacaaacct tacaaacttt acaacttcca tttaaatttt
     3961 ctctagcact ttttaataat tcattatatt ccatctttaa tcccccatta actatagtaa
     4021 tttatattat atacattata tcatattata tttacagtat atttttcaaa aaactttttc
     4081 tgtggatagg agttatgcca tatttttcaa tagcttcata gtgttcttta gttccatatc
     4141 ctttgtgaga tttaaaccca tattctgggt atactctatc atattggtac attatactat
     4201 ccctagtaac tttagcaagt atacttgctg ctgctattga tatagatttt gaatctcctt
     4261 tcacaattgg attttgagat atgtcaatac ctggtattgt tgctgcatca actaataaat
     4321 aatccggtgc tttttttaaa caatttatag cctttttcat tgccatatat gtagcattta
     4381 atatattaaa ttcatctatt tcttcattgt ttactatacc tattccataa tctagtgctt
     4441 cttcttttat tacttcaaaa agttcatctc tttttgcctc actcagtttc ttagagtcat
     4501 ttacaccttc tattttggta tcttttttaa atactacaac agaagctaca acaggtccag
     4561 caagtggtcc tcttcccgcc tcatcaattc ctccaatata taaataacct ttatcatagc
     4621 cttcgttctc aaatatattt atagtttcca atctttcttc ttcttttcta atattatcca
     4681 gcttttttgc tagttttata gctagacctt gtacagattt tctttcatca actcttaaca
     4741 gctctatata ttccatatat ttttctactt ctaaagtctc tattatttct ttaatctccc
     4801 tcacactttt atcctgcata ccattccctc atctttcgtt tttaaaaatt taatttttaa
     4861 atatattaaa attttttaat tagagtttaa ttgcatccac tatagccaca ttttaaacat
     4921 atactacatc cacctgtatg tgcaattcct tcacttccac actcagggca cacaaatttt
     4981 tcatcttctt caaaattttt atttattatt tctttggctt tttcatcttt attatctttt
     5041 tcttttttat tatttttata atattctttt ttactatttt cttttttatc ttctttatta
     5101 ttttcttcct ttttttcatt tattaaaatt ccactccttt tacatccatc tctaaatatt
     5161 gttactcctt taagaccagc ctcccatgcc ttaatatata tattgaaaac atcttcaact
     5221 gtagtttcaa aaggtatatt tattgtagat gatatagaag catcaatata cttttgccat
     5281 actttttgca tgtttattct ttcttcatat cctatattca tagcagtaac aaaaaaatct
     5341 ggtaaacatt cttcatctgt aatacctttc ttgtccatat actctttaac aattggagta
     5401 tatactttat aatacacatc ctcatcatgt aagctttcag tttttcttat atatgaaaga
     5461 ttaaatatag gttctattcc accactgata cctatcattg tagatatgga acctgtagga
     5521 ggtatggtta aaagttgtga atttctaagc ccatactttt taaccatttc cttagtgtct
     5581 tcatttagat tttctataaa aaacttagat ttagaaatat tttcatattt atactcacta
     5641 aatgttccat attctttagc aattaatgct gattgcttca cagcttcatt taacatagtc
     5701 ttaccaatta aatcacatag ttctaaagat tcatcagaac catatcttat tcccattttt
     5761 ataagcatat cagctatacc cattacacct aaacctatct gtctatactt atgtacacta
     5821 tctctttgtt cttgtaatgg atgcaagttc aatccctcat ctaagacatc atttaaagct
     5881 actacagctt ctctaacgca agattttaat ttattaatat caaaagtagc atgttcacca
     5941 aatggttgta ttataaactc tgacaagttt attgagccaa gtagacaact tcctccactt
     6001 ggaagtggtt cttctgcaca aggatttact cctgaaaagc aaaagttctt atcttcactc
     6061 ataaaattat atgtttttat tttatcccaa aacaatatac ctggttctgc ataatcccag
     6121 ttatttttca caaatttatt aaataaatca taagcattta ctatcttaac tattttttct
     6181 ccagtagttt caacttcaaa agtacaatta tataactttt tttctcttac agcttgcata
     6241 aattcatctg ttactctcac agaaatattt gctttagtaa ccttctccaa atctgttttt
     6301 atatctataa agtcttctat ctctggatga tttacatcca tagaaatcat aagagctcct
     6361 cttctccctc tttgaccaat taagccagtt gtcatactgt aaagttccat aaaagagact
     6421 gctccagtcg tatgttttgc agcattgtta actttcgctc cttttggtct tagctttgaa
     6481 atatcaattc caactcctcc accataacta aaagttctag caagtttttt tgcagtatca
     6541 aatatatctt ctatgctatc ttttggtggt tctattacat aacaattaga atatgttact
     6601 ttttttccct ctttctgtaa tcctctattt gctaaaattc ttccagcaaa tagaaatctt
     6661 ttttgcctta ttagttttga tattcttgaa ctattatttg aaattctctt tatccattca
     6721 tcaaaactct caccattatg tctatacttg ttcttccata ttgatttttg taattcatca
     6781 aactcccaag ctttagtcct tatttcagag cgtctttctc tatataatat gtagtgtttt
     6841 gctatatctt ttctatttga ttccataagt agtacttcaa ctatatcttg tatttcttct
     6901 acggataagc atttttccat agaattatag tcattagcta tttttcttga tatatcatat
     6961 gccaactctc tatctactcc accttcacta tttatactag ctttatagac agcatcttca
     7021 atcttacttt tactaaaaag ttcttttgta ccatctcttt ttattatgta tttcattttt
     7081 aaacctccaa taaattagta ttaaattaat acacacatta actataactc taaacaaact
     7141 ttttatatat ttgtatattt tcatattaat tatctaacat aatatatatt atactatttt
     7201 atattcaata taacactcct tatagttttc ttaacttgta aaatatcttc ctaaaaaact
     7261 caaaaataat ataaaaaagt atataaaagt caaattatac tctt
//
LOCUS       DAELJO010000181         7286 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELJO010000181 DAELJO010000000
VERSION     DAELJO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.183"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(152..1402)
                     /gene="cme"
                     /locus_tag="KPB88_003178"
     CDS             complement(152..1402)
                     /gene="cme"
                     /locus_tag="KPB88_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG0055417.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAITMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1488..2234)
                     /locus_tag="KPB88_003179"
     CDS             complement(1488..2234)
                     /locus_tag="KPB88_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0055418.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2425..3447)
                     /locus_tag="KPB88_003180"
     CDS             complement(2425..3447)
                     /locus_tag="KPB88_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0055419.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIVSQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCNPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3447..5867)
                     /locus_tag="KPB88_003181"
     CDS             complement(3447..5867)
                     /locus_tag="KPB88_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0055420.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHNDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECHGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(5953..6873)
                     /locus_tag="KPB88_003182"
     CDS             complement(5953..6873)
                     /locus_tag="KPB88_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055421.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         2435 a         1163 c         1044 g         2644 t
ORIGIN      
        1 gggactcaaa ataaagataa acctattttt caatgttgaa atgataaaat atatttgatt
       61 tcatataaaa aattttcttc ataaattaaa aagtccattt taagccccct catttttaac
      121 cagataaatc gagttaaact atttaaatat attactcgga ttcatttgac ttagatattc
      181 cttgcttgta aaattgtttt gaatagggga tagccaaagc aaaaaataaa agtataatcc
      241 ctgctatgat tattaaatta ttaatagaaa ttacatctgc cattggtccg aaaatcatca
      301 taccaagagg aataaaacca gtatatataa taataggtaa gctaagaact cgacctagca
      361 tttgtggtgc tacatgctct tgcaacatag taatagctga tgcttgaatg attggaattg
      421 aaaaattcgt aaaaaacata aatataaaaa attgccaagc ttgtgttaca agacctagaa
      481 taattgaaaa aatagcatag gataatattg ctagaaaaaa ggtcttattt ctatttttaa
      541 aaccaccaaa aactcctata atcaatccac ctaaaaccat acctaaaaat ccaatcattt
      601 caataataga caggtatata taacccttgc caaatgttcg ttcaataaga agtcctataa
      661 gaaatcctga tggaacacaa agaagcatga atgctccata agtaaaaagc acttttctaa
      721 tgaacttatc tgaaaaacag tactttatcc ctgataccat atctgcaaaa aatgagactt
      781 tttcagtttg ttttggtata tcatgtttag gaattttgat gaatatcaat aatacaatag
      841 caataattgc agtaaataca tcaattaaaa gaacttgatt tattttatta ccaaatccaa
      901 gtattgcagc agctacaata ggaactataa attgtactat agattgtagg ctactattta
      961 taccattaaa acgcattaaa tgttcttctg gtacaagttg aggaatcatt gcgtttacag
     1021 caggtgtttg tacaccactt cctagtgaac gtataacaga tattactgat atggctatta
     1081 attcacttgt tgtgccattt gatgacatca taaatagaat tagaactaat gtagatatag
     1141 caattgttga atctgataca attatgagta cctttcgatt gtagtagtct gcccatacac
     1201 cagcaaaaaa agaaattatc atttgaggta taaaggagca tacagttaaa actgttaccc
     1261 atacacctga tgatgtctga agtgtgacat accatatcat tgccatttgt acaagtgatg
     1321 agccaaataa ggaaatatat tgacttgcta aaaaaagaat gacttttttc ttccatgtag
     1381 aattagtatt tttattttcc atcctattcc tcctttgatt tgtgtgttga atttttcttg
     1441 ctcttaagga taacacacaa atcaaagtct ggagttagag tggagggcta ggaaaattgt
     1501 tttttcataa tttgaagatg agatttcatg gtttttgcat tatgttcagc ttctttcaat
     1561 gaagttatca gtttgtcaca cactgagata atttcgtcat catcttttgg agtgtcaagg
     1621 acagttttaa tattagtatt aggattagaa gataaagcat ttaacattcg taaaatagct
     1681 gaaagagaat agtttgcaca acgaagtgcg cttataattt taagtcgttt tatatcttca
     1741 tcagtatata cacgataacc attttgtttt ctttttactt ttaacaaacc attcatttcc
     1801 caatttctta aagtatctat tgtaacattt aagtgttctg cagtttcttt tctagttaaa
     1861 aagagtttat ttgtttctgg agtatttcca tttaaaatct gtgcaacaat agtaagtgct
     1921 tcctcggcat tagatttttc tatttctaat tgttgcaagt attgatttac aagactaata
     1981 gccttatcaa agtccctatt cgctgatgtt cttacaatct caagggcatc ttttcttaat
     2041 cccctttgta ccacttcaat tttcaaggct gtacgagcaa atctaaattg ttctatatga
     2101 aaatcattaa agatacgata accattggta ttacgcttag gtttaggtat taagtttagt
     2161 tcttcatata aacgtactgt attaggatgt acaccaatta aatctgctac ctctgatgtc
     2221 gtgtaagtgt ccattagtat tcaactcctt ttagatatat agtattagtc ataataacac
     2281 caaacaaaag tctggtgtta taatggaggg tttggttatc taagagtaat taatttatga
     2341 aagagtaatt aatttatgaa atagtaatta atttatgaaa gagtaattaa tctatgaaag
     2401 agtaattaat ctatgaaaga gtaattaatc tattatagaa gtaagtatat atttttcaat
     2461 aaactcccat ctatcacgag gtttaaacac tattttagat ggtatggcta ttactaagtc
     2521 atattgtgga atacaacaaa tagcatttcc tccagctccc attgctgaat accacaaaac
     2581 accatctttt ttacatagcc accacagata accgtattca ttactattca ttttaactga
     2641 ttcttcaatc cacgatttag atataatacg tttattattc caaataccat catttaaata
     2701 cagaaaacca aatcgcaaca tatctctagc actcaatgta agtccccagc cacctataga
     2761 ataaccctgt ctatcttttg cccaaccctt aagatttttt ccaaagaaat cttcaaaacc
     2821 aaaatctttc atatcataat ctggtataac tttcatacca ataggtttaa ataagtattc
     2881 attagcaaat tcacgagcag attttccagt agtttttgtt ataatagctg aaagtaggtg
     2941 cgttccagct gaagaatatt tgaaagtacc aatttcgcct ttttgaccaa gttgattcaa
     3001 ggtatatttt atccaatctt tttgtttgca aagtctttct aaagactcag tcatattttt
     3061 aaaagggtat ggtgcagtca ttgtaagtag gtgacgtata gttactgctt ttttacatat
     3121 ttcagaagga ttacaagtat actcagggaa aaaatcaaca accttttggt caacatcttt
     3181 tatatatcct ttatctattg caatacctat taaggctgat ataatacttt ttgtaactga
     3241 tgcaacatga aaagtatcat cagcattata tccattatag tacttttcaa agacaacttt
     3301 tccttttctt gcaataatta ttccatttat attactatat tgtgagacta ttaaaggttc
     3361 aagttgtgag agcttttctg aactcatgcc aacacttgaa ggatttactt tttcaaatat
     3421 taaatctaag caattgtttt tattcattaa agatacctcc ctgttataga ttttggattt
     3481 tcttttagag tttttggaga accttctgca attatataac ctcctttgtc accaccttct
     3541 ggaccaagct caacaatcca gtcacaaaca tttaatacat ctatatcatg ttcaacaaca
     3601 attacagaat ttccattttc aaccaattca tctaataatt gaatcaactt agaagtatca
     3661 tatagactta gaccagtagt tggctcatcc aatacataaa ggatattacc atttcgtttt
     3721 ttaccaattt cttttgcaag tttgattctt tgggattcac caccacttaa agtcggtgta
     3781 ggttggccta attttatata ccccatacct atttgttcta atacatttaa gtgtgaaaca
     3841 atatttggaa tatctttgaa gaaatgtaca gattcagaaa cactcatatt taaaatatca
     3901 tgtatatttt tatttttata tttaatagat aaagattcat cattaaatct ttttccatga
     3961 cattcactgc atatatggtc tatacttaga ttatttccca accagacctt ttcatatcca
     4021 ctaccactac atttaggaca agcacctttg gagttaaatg agaaatgacc agctgtaaat
     4081 cctcgttcta acgattctgg ctgacctgca aatagtgtac gtatcttatc ccatatacca
     4141 atataagaag caggagttga gttcatattt ttgcctatag gggattgaga tatctctgaa
     4201 tatccagaaa tatgtttagt accaatcagt ctatctgcaa tagtttcaac aactgcataa
     4261 tcgtcttcct cacctatttc actctctgaa gaatttattt gattattgat agtatggtta
     4321 ttaaattggc ttctaagaag aggaagtagg gtgtctgata taagtgaact cttgccacta
     4381 cctgattttc cagatattcc aaccatagca cccaaaggta atgatacagt tacattttta
     4441 agattatttg tatttgcatt ttgaatagtt atacaaggta ttttatcatt atgtgtaatg
     4501 tttttattgc ttgttctatt aggcattgga tattttccag ataaatactg cccagtaagt
     4561 gatttgtcgg attgaagtag accttctaaa tctccttgat atactaattg accaccttca
     4621 acaccagctt ttggaccaat atcaataata tgttctgcca ttttaattgt gtttttatca
     4681 tgctcaacta ctatgaccgt attgcctaaa tctttaagtt tctttattga ttttaagatg
     4741 tctatttttt ctgattcatg caatccagct gttggttcat caagcacata aattaatgag
     4801 tccatctctg aatcgagatg tgaatttaaa aatagtcttt gcatttcccc accactcaaa
     4861 gttgacattt ctcggtataa ggatagatga cctaatcgtg attctattaa atgttgagtt
     4921 ttggataaaa tatcatttac aagatttgta ccaaatgtgt ttaaattacc ttgatttatt
     4981 aagagtttaa ggaaatcatc aatttctaaa atcgtcattt ttccaagttc tccaatatgt
     5041 tttccgttta aaagtactct acgtgcttct tcaccaattt taaaaccatt gcaatctgaa
     5101 caggcagtca tagtataaac tccatttaaa tcttcgcccc ttttgtaacg attgtgaaat
     5161 acctttgata aagaggaact ttttttacca ttttcataat gaccatagag tacttcattt
     5221 cttatttctt caggtagttg taagaattgt atgtctaaat aatcgttgaa ttttttagaa
     5281 aatactctca aatatcctgg tgtaatcttt gctctatcta agacttgttt tagggtgtca
     5341 tgcttatcaa ggactaattt ttcaatatta atctcaaaat atgcaccatg tccagagcac
     5401 ttcatacaca ttccatctga agagttgtat gagaaataac ttggtcttaa tcgttcttcc
     5461 tcatatccac aattattgca aattagatta tcgcttacag gagtatcaca catagtacac
     5521 attatttgac cttccactga atacaatagt gtaagcatgt tgagaatatt ggttcttgtg
     5581 cctactgttg aacgaggatt gctctggcga ataatatttt gttgaacagc tatagtggga
     5641 gctaaacctt gaatattatc aaacttatct tcactatcaa tacctgcaag tatacctaaa
     5701 gattgcagat attgtttacg cccttcctca aatacaatgt caaacataag gctagactta
     5761 cctgagccac tgacacctgt tgcaacaaca agcttatctt ttggaataga aacatctata
     5821 tttttaagat tgtgtatctt tgctccatta atttttattt ctttcattgg ttcacctcat
     5881 tttatcaatt ttatcaaacc gtaaaatagg gatatattaa tccttaatac aagaatagat
     5941 taggtgaggt tattaaaaag gttctatagg taaatatata tcgaccaagt gtctattttg
     6001 agggtgattg tgtgggttgt taagatatac ttcaaaaggg aaggaggcac gtagcttata
     6061 attaccattt gatagccatt cattatacat aaaatcccac gcatcactat attcagattt
     6121 tgaaatatca aaatgtccca caagatattt tccagaaggt attatcatat tgccaagatt
     6181 gtcattctct ttgacaacaa tgttatctgg aatggtcatg caaatactag tccttaattg
     6241 atgctcctgt gtaaattgtg gattatcatg ataaatggct aatatttgtg tgttttcaag
     6301 tccaagtaga ttctgctcac ttgcatatgt gaataaccgt tctaaaagtt ttgggaaagt
     6361 actggttaga ttaccatatg aaccagtata tctagtgtaa atagtattca tattatcaag
     6421 ttctagaatt tcaatttcac ctttaacctc tctagtattc ttacatttag gttttgatat
     6481 gcttctattg tactgagaaa ttttatatgg ttctttgcaa ttcttgctat aattgtttct
     6541 atattttata gggcttattt tatagtaatt tttaaatgct ctcgagaaaa ctgctgaatc
     6601 agtaaagccg aagtagtgtg caatatcagt aacagtcatg tcagttctat gaataagaaa
     6661 agcagttgca ttttctagtt ttagcctatt tacatattgt gccaaagttt ctttttctat
     6721 tgctttgaag attctatgaa aatgaaattt tgaaaaacct gctatattag aaagttcatc
     6781 aagagaaaga ggttcatgta aatgttcttc tatatagtct tgcactttgc aaatacgatt
     6841 tagatattct tttttacttt tagcatttgt catatatagc cctccttatt gtgtaaaatg
     6901 tgtatcaatt aatttatagc aatatagtaa taaaatttta tgtttttgta atttatgata
     6961 aataagatag cgtttaattt tttataattg ataaaagata tttataatta ataaaagata
     7021 taagatgcct tatatttgtc tttgcatata agtatagtct tcgtaataat tagtatacca
     7081 tagtgattta tttttaattg tgtatttata taaatacaca acttatgata tggacgaatt
     7141 attatgcaaa taagctgtaa ttaaataaat tgcaatataa accagttgaa acataagtta
     7201 gggaaatatt atcaaagtta ttacttagca taagagtata gatggaataa aataagatta
     7261 aaaaattaca tactaaattt aataaa
//
LOCUS       DAELJO010000182         7186 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELJO010000182 DAELJO010000000
VERSION     DAELJO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.184"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(66..1994)
                     /locus_tag="KPB88_003183"
     CDS             complement(66..1994)
                     /locus_tag="KPB88_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0055422.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2228..2680
                     /locus_tag="KPB88_003184"
     CDS             2228..2680
                     /locus_tag="KPB88_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055423.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDANHLVKYITNKMKEE"
     gene            2683..2991
                     /locus_tag="KPB88_003185"
     CDS             2683..2991
                     /locus_tag="KPB88_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0055424.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3105..4211
                     /locus_tag="KPB88_003186"
     CDS             3105..4211
                     /locus_tag="KPB88_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0055425.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4286..6964
                     /gene="mngB"
                     /locus_tag="KPB88_003187"
     CDS             4286..6964
                     /gene="mngB"
                     /locus_tag="KPB88_003187"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0055426.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         2856 a          898 c         1062 g         2370 t
ORIGIN      
        1 tactatttat gctaaaaaca aaaaatattt tattaaagtt tttaaaactt atgtaatata
       61 aaaatctata ctatagactt gattatggaa agaaaattat caaaatcatt acaattagat
      121 atactatctc taatatcatt gtcatgaatt atctttaaaa gtatatcata aatggagttt
      181 atttttatat aatcatcttt atttatagct aacatcatga ttaatttaac agtagaattt
      241 tcactccaat atattggttc attcaatatg gaaatacaaa tcttagttct tatagagctt
      301 aattccatag gatgaggtat agctaaaaaa tcatctatat tggtagaaga taaactttct
      361 ctgtaaaata cagattcagc aaatgatgga aatacaattt cactttgagt taatttgttg
      421 cacatcaact ttataagctc atctttatct ttgattttag gattaacaat aaataaattt
      481 ttatcaaaga aattgtccaa aagattacta taaatatggg aattattagt gattgactta
      541 gatatattct ctatatcttt ttttaatagt tttaaatcca ctaatacaat aggaatacta
      601 tcatgattta aaggaattgt cgatattatt atatctacat ctgacaattc actagctaaa
      661 aattgattaa atgatagaca ttgaagtata tttattttat catgaaaaac tttgtttaat
      721 tgaacctcta ataacctact tgaaccatac cctgaaccac atactaatac tacgttttta
      781 cacttaatat tactttgaaa aaatctctct attccagcac ctatatgcag ggatacatat
      841 ccaatttcat cttctgttaa agagtaaata ctatttttaa aaactttttc tatagcattt
      901 aaagttattt caaatgctaa aggataatta ctctttattg tatttataag gggattcacc
      961 ttatttaaat tataagattt agaattgaga attgatttaa agtgcagtac taaatcatca
     1021 aataatacac tatcatttct aaggtcaaaa gtatattgat tgttgattac ttctaaaatt
     1081 tcttcaacat actctataat tttagtatct accctattgg aaacgttatc caaaagagaa
     1141 cttttagtta taaaatgatt atatagataa actctgtctg cctttgaaat aattatagta
     1201 tatttactct caatataatt aaatatattt tcaacagttg tattcgattg aaaatctgtt
     1261 aaaacaacat ctaaaacatt atctaagcag taaccatcaa gtattctact tacagtaata
     1321 gctaaatgga taataaagtt ttttagatta aaatctagga aattgatatt aaatttatta
     1381 atttcactta acactaccct ttgaagctct attaaatcta cattattaaa taattctatt
     1441 tctctatctg taattccaat tatatagttt tcaaaattcc tatttatcat attattagaa
     1501 atacatcttc gaatatctat ttccttacct tcaactttaa ctccataata tggcttagaa
     1561 actaatatta aattatactt tgacaaaata ggctttattc tttttatgtc atttagtatt
     1621 gtaaatttac tcacatacaa gctattagct aaatcttcta gttttatata tgtattgtta
     1681 tagagaatat attttaaaat atattgattt ctttctatag gagaatctgg tatttcactt
     1741 gaatccataa tatcatcaga tattaatgct agatactgtt ggtataatgc taaattaaga
     1801 atatctatat aatatccttc tccttttttt attttaataa tagcaccatt tttctctaaa
     1861 gttccattta tagaagtaat atcatttctt atagttctct cactcacttt taaatgatta
     1921 cttaaagttt tacaagtaat tttatcatca ctttcattta ataatttaaa caacgtgagc
     1981 aatctcttgt gcataaatat ccccccttat agaatttctt ctaaattata ctatttacac
     2041 tcatataata caattactct aataaaattg tattataata atttatttcc acttctatat
     2101 ggaaattagt tattatcata atataaattt ccatgtcgaa gtggaaatta gttatattta
     2161 atttaattag gataatgatt acaatataaa tataagttaa gacattatga atggagtgga
     2221 aaaatatatg gtactacaag aaaaatatat atttatagat aaagacttaa aaagcaaaga
     2281 agaagtatta aattttattg cagatagtgc ttatatacta ggtattacaa ctaataaaga
     2341 ggctctttta aaagaccttc taaaaagaga aaatgaattt ccaacaggaa taaaagatgg
     2401 atttgcgata cctcatacaa aaagtgtaaa tgtaaaggaa gcttccatac tctatattaa
     2461 aacaactaac gagttagagt ggaaaacttt agatgatagt aaggttagat atgtatttag
     2521 tttgttagcc ccacataaaa atgaaggaaa tatacatttg aaaactctat ctaaattagc
     2581 cacatgttta atggaggatg attttacaaa tgaagttaaa aatgctagtg atgcaaatca
     2641 tttagtaaaa tatataacta ataaaatgaa ggaggaataa ttatgaaaat agtagcaata
     2701 acatcatgta tagcaggatt agcacacaca cctatggcag caaaagcttt agaacaagct
     2761 gcatcaaaaa tgggtcatga aatcaaagta gaacaacaag gtgctatggg acgagtaaat
     2821 actataacaa aagaagaagc acaagatgcg gactttatac ttatagcatc tgaccaaact
     2881 atatctggaa tggaaagatt tgatggtaaa aaagtgataa aagtcaaaat aggtatagcg
     2941 ttaaaaaaac cagaagctgt cttaacaaag tgtatagaag ctatttctta ataaaaaaat
     3001 ctcactctaa tatagaatga gaaaaatcaa aaattaaaaa attgtctact gtaattataa
     3061 catattatca aaaaatataa caaacttata taggaggatt aaaaatgagt aaaaaaaata
     3121 aatctctttt aagtgaactt aagggacact taatgacagg tatctcatat atgttgccac
     3181 ttgtaatagg ctcatcttta gttgtagcta tcccaaagct tacagcttta gcagtgggta
     3241 taacaagttt agatgcttat aaaaatgtat ctggttttta tcatattctt tacttaatgg
     3301 aacaagttgg ttggactggc ataggtctat taaatacggt attagcagga tttatagcat
     3361 tttcaatagg tgagaaacca gctatgggtg caggttttat tggtgggctc atagcaagta
     3421 atactaatgc aggattctta ggagcagtaa tagcaggttt ctttgctggg tatgtttgta
     3481 agtttttaaa gaaaaaaatt aaaatatctg ggtcagcatc aggaatgatg ccacttataa
     3541 taatgccact tataacagtt ggattaactg gttttttaat gagtgttgta ttagcaggac
     3601 ctttaggaaa cataaatact tcattaaatg cttgggtagc tgaaatgtgt caaaatggaa
     3661 caaattcggt ggtattggct ttaattttag gcgcaatgat aggattcgat ttaggtggac
     3721 cagtaaataa agcagcttgg atggcaggaa atgcattatt gttagaggga atctatctac
     3781 cagcgatatt ggtaaactgc gcaatagtta tacctccact aggatatggt ttagctacat
     3841 tcataagaaa atcaaaattt tccaatgcat tagctgaaac aggaaaaggc aatatagtta
     3901 tgggtataat tggaataaca gaaggtgcaa ttcctttcac tttaacaaat ccattaaaat
     3961 tagttcctgt taatatgata ggatgcgctt tgggtgtagg attatcagct ttgttaggtg
     4021 tacatgctat aatgcctcca gttggtggat tatatggatt tatatcaata ggttctggat
     4081 gggcttactt agtaggtgca atatttggag ctttagtaat agcaatatta tcaacaactt
     4141 tagtagactt caatgaagat gaaactacgg aaaatacaga atttgatgaa atagagttag
     4201 atataatgta aattaaaaac ttaattttag ataattgaga attgagtgta ctctcaattc
     4261 tcaattaaat tttgggagga tttttatgaa aaagacaaaa gtacatgtag ttccacacag
     4321 tcattgggat agagaatggt attttacaac atcaaggtca aaaatatatt taatgaaaga
     4381 ttttcaagat atattagaca tattggaaga aaaagaagac tttaaatact ttactatgga
     4441 tgcacaagct tctctcttag atgattattt aaaatggaga cctcaggata aatatagaat
     4501 aagagattta gttcaaagaa gaagattagt tattggacct tggtatactc aaacagacca
     4561 attggttata tcaggagaat ctataattag aaacctttac tatggaatag atagatgcaa
     4621 tgaatttggt gaacctatga gggttggata tgtaccagat tcttttgggc aatcagctca
     4681 aatgccacaa atatacagag gttttggaat agatagttca ttattttggc gtggagtttc
     4741 tgatgatatg gtagatacaa cagagtttat atggaaaggt tctgatggtt caaaagtatt
     4801 agcagttcaa ataccatttg gttattacta tggaggtaat ataccagaag atgattcaga
     4861 tttaaaagaa tatttaaaag atataatcgg aaaacttaag gttaaagctt ctactaataa
     4921 cgtatatttt ccaaatggat ttgaccaagc accaataaga aaaaaccttc ctgatatatt
     4981 aaaaaaagct aatgaaatag ataatgaaaa tgaatacgaa ataagtgata tagaaaacta
     5041 tatagattca gtaagaaatg aaagaaatga atttacagag cttaaaggtg aatttttaaa
     5101 tgctaaacat atgagaatac acaaatccat attctcctca aggtcagacc ttaaaataat
     5161 gaataacaaa ttagaaaatt atattacaaa tgtacttgaa ccattattaa cattaagtta
     5221 ttcacttgga aatgaatacc ctcatctagt tataaaagat atatggaaac ttatgtttga
     5281 aaatgcagct catgacagca taggttcttg taatagtgac acaacaaatg aggatgtata
     5341 cacaagatac aaacaagcca gagatttgag tataaattta ttagatttgc atatgagact
     5401 tataagtact caaatcaaaa ataataatga agaaataaca ttaacagtat tcaatggatt
     5461 cccagaagtc agaaatgaag ttgtagaatt tgatacttat attccaggtc aagattttgt
     5521 gataaagaat aaaaatggag aaattcttaa ttatataata aaagaaaaag aagatattac
     5581 aaattatata ttaactcaaa caataagatt aaacccaagt aaaaaaatat atatacccaa
     5641 aactgtatat agagcaaaaa ttgcactttc agtaaaagat ataccaccaa tgggatatac
     5701 tcaactagtt tttgaattgg atactaaaaa taaatgtgaa atagaaacaa gtaattgcaa
     5761 ggaaatagaa aacaaatact acaaaataac agccaataaa aatggttcat tagaaatatt
     5821 agataaagaa tctaataaga cttataaaaa ccaagctgta attgaagaca atggagatga
     5881 tggagattca tacaactatt ctcctccaag aaaagatatt tatgtttcat ctttagattc
     5941 tgtatcttca gtagaagtgc taaaatcaaa cattcaagaa gaaatgatac ttaaatattc
     6001 attgaccata ccaacttcac tggaagaaag ggctataggt aaagtttctg tgaaaatgcc
     6061 agtaacaatg aaaatcacct tagaacctaa agaacaaata attaagttta atgttaatat
     6121 aaaaaataac caagcactat ctcatagagt aagagtttta tttaatacag aaatagcatc
     6181 taagttttct atagcagatc aacaatttgg aataatacaa agaccaacag tattagaaaa
     6241 agaccttgca ttgtggaaaa gagacaatta ctcatggcaa gaaaaaccta taactatcga
     6301 accaatgcaa agttttgtta ctttagagga tggacaaaga ggaatagcat taataactgg
     6361 ttgtgtaaga gaatatcaaa tagttggtga taatttagat acaatagcat taacactatt
     6421 caggtcattt ggatatatgg gaagagaaaa tttactttat agaccaggaa gagcttctgg
     6481 agaaaaaata atagcaacac cagatgcaca attacttaaa gagttaaact ttgactttgg
     6541 tctttacata tataacaata aatttgatga agcaaatgta gctaatacag ctaagagatt
     6601 cttaacacct atacaaatat atgaatatgc tgatttttta aatggtagac ttatatttgc
     6661 attcaatgac gaaaaacaaa tatatgaaaa tgaatttagt ttgatgaatg taaataattc
     6721 aaatttcaca gtaagtgcaa taaagaaaga agaaaaagga gatggaattg tagttagaat
     6781 cttcaatggt atgaaaaatg aggatgctaa gggaagtctt aacatcaata aaaatgtcaa
     6841 tgatgcttat agtgcaatgc tcgacgaaat ctatgataat actagcaagc ttaaagtaaa
     6901 tactaaaact gtagaggtaa attctttatc acattgtaaa gtacaaacaa tagtaattaa
     6961 ataatataaa atagcttata caagatttca aaataaattg ctagtactat gttaatagta
     7021 aagccagata ttatgctatt tataagctct gtaacactaa ataacatttt gtaaaataaa
     7081 aaggtgaacc ttgtatatta ggattcacct tttttaaata aatcaacatt ctgttttaaa
     7141 ataacattta taagctgttt tgaaataatg cttacaaatt atttta
//
LOCUS       DAELJO010000183         6948 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELJO010000183 DAELJO010000000
VERSION     DAELJO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.185"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(54..914)
                     /locus_tag="KPB88_003188"
     CDS             complement(54..914)
                     /locus_tag="KPB88_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0055427.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            1407..2810
                     /locus_tag="KPB88_003189"
     CDS             1407..2810
                     /locus_tag="KPB88_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055428.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLKKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIIFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3029..5506
                     /locus_tag="KPB88_003190"
     CDS             3029..5506
                     /locus_tag="KPB88_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0055429.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            5521..6207
                     /locus_tag="KPB88_003191"
     CDS             5521..6207
                     /locus_tag="KPB88_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055430.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVAKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            6481..>6948
                     /locus_tag="KPB88_003192"
     CDS             6481..>6948
                     /locus_tag="KPB88_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055431.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELL"
BASE COUNT         2621 a          793 c         1033 g         2501 t
ORIGIN      
        1 aataaatcgt attttaatta aaatagtata tatacttaaa atattaattt ttattagttc
       61 tctaaatttt taaatccttc tccaagaacc tcatgtacat ctgtaacaat aacaaatgca
      121 gtagggtcag ttcttttaac taatctcttt agataaagtt cttgtttttt agaaactacg
      181 actaatagaa cttctttatt ttctctagtg tatccacctt taccatcaag aatagtaaga
      241 cctctatcaa gttcttcagt tatagcttgt cttaaacttt caggttcatc agagataata
      301 aaaaatgctt ttgaatggtc aaatccttcc attatcatat ctgctatttt tattaataca
      361 caaagtgcta taccagagta aagagcagtt tctaaactac gatttactat accagaagat
      421 ataacaacca ttccatctag gcatgacatg aattttgtag cttttattcc tgggaatttc
      481 ttactaagta tgagtgcaat taagtctgtt cctccagtag aagcatctga tttaaacatt
      541 attcctaaac ctataccaac taggattcct cctgcaatag aagacaataa taagtcatta
      601 gtaaatctta gttttgataa tgggtctgtt aattgaacta tagctgaaaa tacaactgtt
      661 ccaaataaag tttttaatga attttttgta cccataattc taaaagcaag tattactaat
      721 gggacaccta taattaacat gatagctgat actggaagtc ctgtaacctt gtttagtact
      781 aatgatagac cactaagtcc tccaggagca attgtatgtg gttttaaaaa catatttata
      841 ccaatactca ttgatataca accaaaaaat agtccaagca tttcaagtaa aattggcgac
      901 ttttgttttt tcatataaac acctccatga acttgtcaca atttaatatt ataacatatt
      961 tgaccagttt atattatata aaatttcttg atataagcaa tttcaagtat ttcattattc
     1021 ttaatatttt tgaattttta tatagtgttt tcatattttt actgatgttg ttacactcct
     1081 atgaagtttt tatatattat aattttttta ttcttttata aagtatcccc tataaagatg
     1141 gttctatgaa aattttctct atactgttta acgtatatag agattttgtg tataattata
     1201 aggtatttaa tggaaaaatt tatcttactt ttatatgtta tcaaaatgat ttattaaata
     1261 caacagacag cttgataaca tataattata taaatataat ttatgtagat agttgtattt
     1321 ttaaataaat ttttacatat caataattat tgtctatttt atataagcaa caatcaaaat
     1381 aaaagtttaa gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt
     1441 catacattat ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct
     1501 tatcacttat tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa
     1561 aatttacaga aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac
     1621 agtctggatt aaaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg
     1681 gaaatagagt ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg
     1741 aattattagc attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa
     1801 ttaaaaataa taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag
     1861 tgaatatgtt ttttgatgga ggtaaattta taggtggtaa gtcaattata ttttcaatca
     1921 tagggattat atttttaata attttggttt ctggaattgc ttatggttac tgggtaacaa
     1981 agtgcatatc aaatattaca tctgctgtta gcgatatttc aaaacgaagc tatcttccag
     2041 ttaatattaa aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa
     2101 ttcatagaag tgatgagata caaaataaaa ctgatattat gagagaagag tggatttcaa
     2161 atataactca tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta
     2221 cagatagtag tactaattat tcacctgaaa aagtatataa atatgcttca atcatgttaa
     2281 agaatataga atatacaaat acacttataa acgatttaaa attaacctat caattaaaaa
     2341 atggaataat ccctttaaat aaaaataatt atgatttagt aagattcttg aaagaattat
     2401 ccattgatat tttaaattat ccagaatatg aatcaaatat agttgaattt aagagtaata
     2461 tagataatat taactttgat tttgattgta cactattaaa acgagctttt agtaatctta
     2521 ttgtcaatgc ttttacatat ggagataaaa atactaaagt ttgtgtacat gtaacattaa
     2581 atgaaagaat tgaaatcatt atatctgata atggaaaagg gatgtcaaga gaagaagcat
     2641 caaatttgtt taatcgttat tatagaggaa gcaatacaaa acaaaaaata gaaggaactg
     2701 gtttaggact agcaataacg aagcaaatta ttgaagttca tggagggaat atatctgttg
     2761 aaagtatacc tggattaggt agctcatttt atatcacctt tcctatttaa ttaaggttaa
     2821 ttaaggttaa ataaaaaata tattaaggtt gatagattat tatatttata tagtagttta
     2881 tcaatctttt ttatttaagg ggtgatttga ttaaaaatat tataaataga atgataaaag
     2941 tatggaggag tctattagag atttaattta gattaaagta taaaaatatt agattgaaat
     3001 atcttagata aagaaagaag gtgtaaattt gaaaattata ttaaagtata ttttcacaaa
     3061 tattaaagaa cgaaaaatta gaacagcagt tatgttattg tccatagttt tatcaacagt
     3121 tttacttttt gtttcattct caattggatt atcttatgaa agtgcacaaa gaaaaatggc
     3181 aaaaggtatg tatggtacag cgactatatc tgtccaaagt aaaaatccag atatattaac
     3241 caatttagaa gacatacctg atttaaatgc tataaaatca aaggttggtg tattggaaag
     3301 ctctgctatt tataataaag ggggatacta tgaagaattt agtcttatat cagcagactt
     3361 aagtcaacta aataaaatca acaagccacg attagaaaat ggcgatagta ttactgattt
     3421 ttctggtgat aaaattattc ttccaaatcg ctttacatca aaatataaaa taaaaaaagg
     3481 tgattctatt actttgcaga tttatggaaa gtcttatact tttcaagtta gtgatattgc
     3541 atcttatgat acagtatttt tgaggaacac aagaggtgta aatgcactgc tacccaaaga
     3601 gactctatca aaaattataa ataaaggcag tggatataca agaattctta tagagtcaga
     3661 agaagaaatt acagaaaatc tagtaaataa attatccgaa gaactttcta ctgaaaaata
     3721 tatggtatca aatactataa atgaaactaa gataatttca gatgctagac aaaagacaat
     3781 gcctttcttt ttaataagtt tttttgcatt aacccttagt atatttataa tatacagcag
     3841 ttataaggtt attacattag aacgtcttcc ttttattgga acatttagaa gtattggtgc
     3901 caatgaaaaa actgtaactc gtatattgat gcttgaaagt atattatacg gaagtatagg
     3961 aggattaatt gctattccta ttggtgtagt tgttttaaat ttaatgcttc atgggttagg
     4021 aaattctctc gaacaaggaa tatcaattcc agtagtaata tcacccattg gagtgattat
     4081 atctgttata gtagcaataa tcgtttcttc atttagtgca tatatacctg ttaaaaaagc
     4141 tagccatttg ccgataaaaa atattgtact aggaacagta gaagaaaaga atgtttcaaa
     4201 tcgttctata ctttttatag gaagtataat gtttatttta tctattttac ttccaagaat
     4261 atcccctgaa aatacattgt atctagctgg tggattttca ttactaggat taattgtagc
     4321 tactattgtg ttgattcctc ttattactga tatcatgtct atagtttttg agtttgttta
     4381 taaaaatatt ttagggaatg agggtaaatt agctgctaga aatatgaaaa ataacaaaaa
     4441 tataatacag aatatcactt tactatttat aagtatttct gcagttattg caataagtgt
     4501 tgttggtaac tttgtcaaaa cttatataac agatgtgttt cgtgacgcag aattacaagg
     4561 ttttgcagat ggaaagatga atgaagagtt tattgaagat gttagacata tggaaggaat
     4621 taaaaaaata ttacctcttt atgttatgaa taatgagatt tctggtaatg gtgtaacatt
     4681 atcaagacta gagggtacag ataatataaa actatataac tctatgtttg gaatcaatta
     4741 tacaaatttt gagataaaaa aacaagtaat agaagctttt aatgataaac gttctgttat
     4801 tttgaatgaa gatactctaa aaaaagtagg cttatctata ggagatacaa taactctatc
     4861 taatgataag tatgattttt cttataaaat agtaggtagt tttaaatccc gtgcaaatga
     4921 tgtagaagct gttataccat cacattatgc agttaatgat tttgataaaa ctaattatgg
     4981 ttttttagta tatacagcag tcaatccaga tgctgttatg attcagataa gatatttatt
     5041 tggagataca tataattgga gtcgtacagt tgaagaattt aataatgatt ctttaaatac
     5101 tataagtagt tttttgtctc ctatgaataa gatgacctat ttcatttttc ttcttgctac
     5161 agtaggtatt attaataatc tactgattaa ttatattcaa aaaagacgtt ctatagctat
     5221 gtataaatct ataggactaa gtaataaaca aaatataaaa gtaacattga ttgaagggtt
     5281 tacttcaggt ttacttggag ctgttattgg tattgtgata tctatacttg agattcaaac
     5341 tatctttata gttgcaggac caaaaatttc aatgaaacca gatttagatt ttaaaacatt
     5401 tattattgtt gggttattgg gaattatagt aactttaata ggttcgatag tgcctattat
     5461 aaaagggaag aaaatgaaac tgatagaaga aattaagttt gaataattat ggaggttaat
     5521 atgaaaatta cacataatag tttagctgta gaagcaaaaa atattatcaa agaatataaa
     5581 attggaaata caacaactcg agtgttaaaa gaggtatctt tacaagtagc gaaaggtgaa
     5641 tttgtttcta tcatgggtca atctggttct ggtaaaagta ctcttttata tattttaggt
     5701 ggtttagata ctccaacgag cggaaaagtg tatatgaatg gagctgatat atcacatttt
     5761 aatgatgaga aaatgagcat aatacgtcgt agaaatatag gattcgtatt tcaattttat
     5821 aacttaatac caaatttaaa tgtggaggaa aatataatgc ttcctttgtt attagatggt
     5881 aaaaatttaa aagattataa aaatcaatta gatgaaatct tagatatagt tggtttaact
     5941 gatagacgta aacatacacc acgagagtta tctggtggtc agcaacaacg tgttgctata
     6001 gctcgagctt taattggaaa accagagatt ttatttgctg atgagcctac tggaaattta
     6061 gatagtaaaa cagggataga aataatagat ttactgaata aaataaacag agacaatgga
     6121 caaactataa ttatggtaac tcactcacca gaagcagcca aaagtagtag tcgtactatt
     6181 acagtttcag atggtttaat tgtataagga ctaattattg tatttgagat aagcattttt
     6241 aattatataa attagacata tgtaaagtgg atataagtta aatactataa atgtgctata
     6301 atattaaaat aacagataaa agtactatat tttttattga ttattttaat tttatttgtt
     6361 tattaaatat ctaatgagta ttcatactat ctctattatt aaaagcagtt aatgatatat
     6421 agagataata tggattcttc ttactttaat aaatattcac gataggacag gatataatat
     6481 atgttaaaaa aagaaagtat tcattttata aataaaaata ggtcacttat agtttgtgaa
     6541 gacaattttg tttatctctt gccttgtatg caattaagaa attggatatc gaattttact
     6601 atatcgtttc caaatcaaac aataatttct gataattaca cgattattcc tcatggaagt
     6661 gtaactttgg tgttttttta tgatgccaca gggttacata gtctattatt tggtccaact
     6721 actaaaccaa aaacagtagg aaatattgct aatagatgtg atgttatact aattatagaa
     6781 tttcaacctg ctggtttttt cccgcttata ggtatacagc aaagtgaact tattgataaa
     6841 gttgttccat tttctataat taatacttca ttagatttag aaattaaaaa aatatttaat
     6901 gaatctttat ctatagataa attgatttta aagttggaag aattattg
//
LOCUS       DAELJO010000184         6901 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELJO010000184 DAELJO010000000
VERSION     DAELJO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.186"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..762)
                     /locus_tag="KPB88_003193"
     CDS             complement(<1..762)
                     /locus_tag="KPB88_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG0055432.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIVLGIIFYLCRGKKFNLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYL"
     gene            complement(773..2176)
                     /locus_tag="KPB88_003194"
     CDS             complement(773..2176)
                     /locus_tag="KPB88_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055433.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2180..2905)
                     /locus_tag="KPB88_003195"
     CDS             complement(2180..2905)
                     /locus_tag="KPB88_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055434.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSNAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(3017..3790)
                     /locus_tag="KPB88_003196"
     CDS             complement(3017..3790)
                     /locus_tag="KPB88_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055435.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(3796..4683)
                     /locus_tag="KPB88_003197"
     CDS             complement(3796..4683)
                     /locus_tag="KPB88_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055436.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGRYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(4697..5719)
                     /locus_tag="KPB88_003198"
     CDS             complement(4697..5719)
                     /locus_tag="KPB88_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055437.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            6294..6470
                     /locus_tag="KPB88_003199"
     CDS             6294..6470
                     /locus_tag="KPB88_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055438.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2400 a         1103 c          631 g         2767 t
ORIGIN      
        1 taaataaaat aaatacttca tactcttccc tttttctcca ttgtaaagta acattaaagg
       61 tagagaagca atcatcatcc attggtaatt ttgtaaaata gaatttttta aactataatc
      121 tccatttgac attgttgtaa atataaatat acacattaaa acataacaaa tactcaaatt
      181 gaacttttta cctctacaca aataaaaaat aatcccaagt acaatccaaa ttggtccacc
      241 ttctactaaa aatggtactg gtaccaaaat cataaataaa gcaataactc tgttagtgac
      301 tttaaaaagt aaagctactg taaatattcc taaaattata ggaatgatta tcatcaataa
      361 tcctataata aaatatttaa tttctttctc ttttctaaat ttctttatga actctattgc
      421 ttgtaaataa atcgctatta aaaataatgt tgaaaaaatg ttatttataa ttatagctcc
      481 ttctggatgt gaaaatacat tattaattat ttgatttcct atagccataa taacagacca
      541 aatatacaat ctacttatat atttttttct gttactagtg tgataaaatc cttcaacagc
      601 catgaatata aacagtggtg ctgatattct gcctacccaa tgaaaccaaa ttggtatttg
      661 cattgtttgt ggcataaatg ctgctatatg gtcaaatgtc ataaatatta atgctaaaat
      721 cttaatagta aacccactta tacctttttt aaaaaaatcc atatcatatc tcctaatttt
      781 gttttatctc tataataatt tttgttccta ttccaatttc actacttaca taaattttac
      841 ctttatgaat ctctattatt tctttagaaa tagccatacc aagacctgaa cctttatgca
      901 tctcacctgt attagtacct ctataatatt ttttaaaaat atgtttcaaa tcacttttgc
      961 ttattccaat tccattatct tttattataa tttctatatt atcttttttt acaatttcta
     1021 cacttatcaa agttccttcg ctattatgaa ctattgaatt aaatataaga ttggttatag
     1081 cccttctgaa taagattgaa tctacatata cttcaattaa atcttcatta ctttcaaatt
     1141 caatattcct atttttatat ctattatcat ttaaaatatc aattattata tctctgacta
     1201 aactgactaa atttattttt ttcttgtcca gaactatagt attatttttt aatcgagtag
     1261 ataaatttaa atcatcaact aaatctttta tatacttaga cttattatat ataatttctg
     1321 tatactcttg tatttcatct tcttcaaact cataatcttt gtcattaatt atctctgcat
     1381 acccttgtat agatgctaga ggtgtcttta tatcatgtgt tatatttgcc agccattcct
     1441 ctctcaactc ttcattttct tttctctcaa cttcatttac tcttaaagta tcagctaaca
     1501 tatttatatt tttaaaaact tcttcatata tacctctgtc cttaagatat aaactataat
     1561 ttccattagc catagtctca atactccata gtacgcttga aattggtttt gtaagttttc
     1621 tactaaataa atatcctaca cctaaagcta ccacgctatc aattactagt gttataatga
     1681 taactttatt taaagttttt ttaatgttat tttgagaata ggttaaaatt attcttgata
     1741 tatctctact aggtatacct actattatgt ttaaatgttt tttgtttaaa tatttttcta
     1801 gtacaaaatt aacatacttt gtttctatat acttgtaatt attcaccatt tgaaagggtg
     1861 tatatttttt tggagtatcc tttggcacat ttactccata aacttcctct cctaaatcat
     1921 ttaaaacctg tatccaggaa tttgattttt ctaatttttc agctccaatc ttagatagtt
     1981 taaattctcc atctttttca tataaatctt tttcaaaaga acgtgcaaat tcttcaggat
     2041 tatttccaga atctacaact ttaaaaaaac tattagttga aatcacatac aatatagata
     2101 tgatatttat aattactact gatatagcta caaacacaat tgtaaatata aaatttctag
     2161 taatcttcca cttcatattc taatcttcct ttactgctaa tttatatcca agacctttta
     2221 ttgtcagaat atattcaggc tttgaaggat tatcctctaa tttttcccta atttttctta
     2281 tatgaaccat tatagtattg tcaaatccaa cataatcttc tccccacact ttatcccata
     2341 gtttttcttt acttataatt tgattttgat tatgtgcaaa taaagatagg atttttagtt
     2401 cctttgcaga cagtcctata gattttccat cttttataag ttctgcccta ctttcattta
     2461 atatatatgg gccaaatgat ataatttttt cttcattatt atttagattt ttactttcgt
     2521 tttgagcatt actaaaataa cttaatcttc tcaaatgcgc tttaactcta aaagccactt
     2581 ccttagggct aaaaggtttt gtaacataat catctcctcc aatcgctagt ccaagtaact
     2641 tatctaattc ttcggtttta gctgataaaa atattattgg agaattggag gtttttctta
     2701 tttctttaca aatatcataa ccttcgccat ctggtaacat aatatccaat acaatcaaat
     2761 caggattaat acttttaaat aattctaatc cttctttttt agtttttgca gtatatatat
     2821 tattaaggcc ttcttttttt aggacagttt ctaaaagctt caatatttct acttcatcat
     2881 ctattattaa aacacgattt tccataaaca acctcctgta ataatttaca tatatattag
     2941 catatatttt atctaaaatt catttatctt atttttaatt aaactaaaat ataaataaaa
     3001 ttactattat ttttagttaa aaccttttgc ttgatgtaaa attttcatat gttattttag
     3061 ttaacactat cattaatata attgagatta ctatagaaaa aactatcaaa atacttggta
     3121 cattatactt aaccatttgt tggctcatca taatccaatt tggagtcact gacataaatt
     3181 ttatagcttt atctggtata aaacctacac taaagtatgg aattgccatt atcaatccac
     3241 ctagtataaa tggaattaca gccgatttaa atatagatga aagtgttaag cctatttgtg
     3301 caaatataaa agaagcaaaa atagtagtca atataacaat tatatacatt tgtattactg
     3361 taagatttaa attactattt ccaaagtacc ataagttatg aatcgattca tttaaatttt
     3421 taaaatcaaa aaaactaaaa cctctaattc ctactaacaa tataaaagaa atattaataa
     3481 tagataaaat taatccatta gctactagtt ttgataatac tactgatttt tttccctttt
     3541 tagttgtatt tattaaacct gtcatattag aatcatattc tttaatgtaa tttccagaag
     3601 ttattataaa cattatcaaa cctattagag gtattgagcc ttctatcttg taaaatatat
     3661 ctgcataagt atctaaaaca tcaaactttc taatcagaaa aaatccaaaa aatattaaaa
     3721 acagccaaat tatccaaaca ctctttgatt ttaatatttt tatcatttca gctttaacta
     3781 aatacatcat ttctactaca cctcccttgc atcttccaaa tcaaaataaa acatgtatac
     3841 atcttcgaat tttgcttgaa ctggttgtat attatcctcg cttggaatat cctctgaaac
     3901 aattctaagt actattgaat taatatcact ttgaatactt acaaccttat atttagattg
     3961 tatatcatca acttcacatt catttttaac ttgaacatta tatactttat tttccatgtg
     4021 acttaatatg tctttgtgtg ttcctttcat taggagtttg ccatctttaa tcattattgt
     4081 ttcctttgca actgattcta tatcggaaat tatatgagta gaaagtatta ttatcttatc
     4141 tctagacatt tttgctatta aattcctaaa tctagctctt tcttgtgggt caagacctgc
     4201 tgttggctca tcaaggacta ttatcttagg gtcgtttagt aaaacttgtg ctatcccaac
     4261 tctcctcttc atacctcctg aaaattttcc tatagactta tttctcacat cataaagacc
     4321 tactaaatct aataattctc ctatcttact atttaaatat cttcccctta aaccttttaa
     4381 agctccaata tactgtaaaa attcttttgc tgtaaaattt ttataaactc caaattcttg
     4441 tggcaaatat cctaaaatat ttctataatc agcatccatt ttaaatatat cctcaccatt
     4501 ataaagtatc tgaccttcat caggttttat caaagttgtt atttgtttca ttaatgtaga
     4561 ttttcctgct ccatttggtc ctagaagacc ataaactcca ttttccagag ttactgtaat
     4621 cttgtcgttt gctacttttt taccatatat ctttgaaaga ttttctattt ctaatttatt
     4681 catcttaact ccccttttat ctacttatat aatctgattt tattactaaa aaacataatg
     4741 acatagcacc tatcaatatt gccatcaaaa gtcctattgc tatattgaca atgtctatat
     4801 tcactttaaa tatcataata tcacctagat aatttcctat tattttatta aaaccaaata
     4861 tcaaatcata ataatttaca tttgatacaa ctgaaagtaa ttttttaggt aatatatgga
     4921 ttaccgacat tatcttagtt atagatataa taaatataaa aaaacttatt gaagatattg
     4981 aagatttact tataaaagac ccaaatatgg ctatcatggc ccatccttca aacatcaaaa
     5041 gctctatgcc tatatttaat attacatact ttgttatact taaattagtt ttcaatgcaa
     5101 ctacattgtc tactattcta aaagattcta agtctccatt aacaggtact ccttgttgca
     5161 tacaagttat aataaatatt cccattaaat aaacagaata aattactaat ggaactaata
     5221 aagcaacaaa tattttagac tttaatgctt ttttcttctc ttttgatgat agtatgatat
     5281 cttttaccga agtaacgact tcatctacat aaatattgga tataattata gatataatga
     5341 ttaacattcc tacatttata agtgaatttg tgactctata gttaaatact ttccaaaaac
     5401 taatatcctt atattcattc acacttagat tatcaagttt tttctttgac attaattcaa
     5461 tttttttaga aaattcacct gtatctgttt gatttgaaaa ctgctttaaa acattaatct
     5521 tattttgaaa tttctcatta cctacgtcga taccacttct cgtatcttct atacgtcctt
     5581 tttctttatc tatataataa ctttctgttt ctaaaacagg cgtaataaag atactaatca
     5641 ttagtattaa cactaatatc cctagagata taaacatact cttagattta gccaattttt
     5701 ttatttccca atttaccaat atatttctcc tcctaaatca ttttaattac acatatatat
     5761 taaaacttaa atcttatata aatataatac taaccttaaa taaagcttaa attattttat
     5821 aatttatagg tgtgtttatt attggagtta catttattat ttttctggga aattatacat
     5881 tttcaattat ttttattatt tttgatattt ttattgattg gaaattttat tgttgttcaa
     5941 attgtagtta ttctttaaat ttaaagatgg agttttttaa gaatagaacc tactgtcctt
     6001 attatggaaa gattattaaa tagttgcata taactttatt tatcttgaat cttattttta
     6061 aaaacaatta taattttaga atattttcta ttctcaaaca agcatgttga ataatgaagc
     6121 atcttctagt attatatact tgtagaataa aactaaattg gtaaaactgg gaatatttaa
     6181 ccttaaggag ttaagttata gaaactagta ctaaaaaatt ttataaatta tgttaattga
     6241 ctaatagaat gcgacttttt atttttacta aattttttag ggggatggat aatatgttta
     6301 ttggagaaat agacaaatgt acacatatac taactgctta catcagtagt tcatatgatt
     6361 attgtaattt tttagataca cagttagatg attttatatc agaatatgga gaaacggtag
     6421 ttgaaacctg cttacatcaa gtcttattat tagtaagtag atacaattaa aactaaattt
     6481 ttctagaaaa acctttttag atttatctac tctaataaat atataatttt ttgtacttta
     6541 taagtaacta taacttctgc taaagtaaag atgtattggg agtacatctt tactttccca
     6601 ttatagaagt taaaagcata caccataaaa tcaatcatcc aatacaaatt atatatttcc
     6661 tatgtctaac ttaatttaca agaatcattc tatatgcata ttctgcaatc cactaaacat
     6721 ggctctaaac ctttactatt ttatacattc tctataaatc atacaaatca tatataaaat
     6781 ccccctaata tgtttaaacc acctataaaa taacaccaat atttagaata ttcaatatca
     6841 actgcataac tggtcaataa aaacgtataa ctagacaaat ttcaatattt atataaataa
     6901 t
//
LOCUS       DAELJO010000185         6875 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELJO010000185 DAELJO010000000
VERSION     DAELJO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.187"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            87..1868
                     /locus_tag="KPB88_003200"
     CDS             87..1868
                     /locus_tag="KPB88_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG0055439.1"
                     /translation="MSQYININSQLDALKLKEFEKLFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1950..2480)
                     /gene="lepB"
                     /locus_tag="KPB88_003201"
     CDS             complement(1950..2480)
                     /gene="lepB"
                     /locus_tag="KPB88_003201"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0055440.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2520..3344)
                     /locus_tag="KPB88_003202"
     CDS             complement(2520..3344)
                     /locus_tag="KPB88_003202"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0055441.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3601..4509
                     /locus_tag="KPB88_003203"
     CDS             3601..4509
                     /locus_tag="KPB88_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0055442.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4653..6098
                     /locus_tag="KPB88_003204"
     CDS             4653..6098
                     /locus_tag="KPB88_003204"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG0055443.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIINDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6409..6861
                     /locus_tag="KPB88_003205"
     CDS             6409..6861
                     /locus_tag="KPB88_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0055444.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2834 a          828 c         1036 g         2177 t
ORIGIN      
        1 tatttactaa atttgcctat aaaaagtata tataaaaata tagttcaaaa tggtataatt
       61 aagtataaca gaaggaggta atgtaagtga gccaatacat aaatataaat tctcagttag
      121 acgccctaaa attgaaggag ttcgagaaat tattttacga aaatatcaat gaggacgtta
      181 aagtagtgcc aaaagttact ccttttaaag gaataaatac cgatttgttg tatgttaaag
      241 atggaagaat tttatttata aaatttatgg atacaacaga agatatattt tttattttag
      301 aagaagagtt acttgaagtt atgaatgagg aatatgaact tttgaaacta aaaatgggac
      361 aaaagaatag aaatataagt tataactacg tttacattat gccatacgtt gaagttgagg
      421 aaacatatga atttgaagaa tttgttaata ataatataat agataaaaat aagcttcaag
      481 atataatgaa taagggttcg ctggaggaat atcttaatga tgagaatgat gaaataaact
      541 taaatttatt tttactagac atatgttcag aatactatat aataaatgat aaacttcatc
      601 taaatgaaaa atttaaaaaa atttcattct acaatgatga ctataagtat acagcaacta
      661 tgatggaaga cgttcaaatt aaagatgttg tttctattaa atatgggaat acacttattg
      721 aaggtggttc tggtataggc aaaacagcaa ttatgctttc tagggctata aaattagcaa
      781 aagtatatcc acatcataaa ttagtcatat ttacacatac aaaacaattg tgcaatgagc
      841 ttcgagagag gatagagtta ttatacaaag ataataacaa tttagaagtt cacacattta
      901 gttcttttat atttaaacta gctaaaaaat tcaatttaat tgtagattat aacatgttaa
      961 aaaatgatta cgaaaagacc tttaataatc ttgtaaaaca agcacagaac attataaaaa
     1021 ataaaaatat gttcaaggct atttttattg atgaagcaga aagcttttta gagtatgaaa
     1081 tagattttat aagggagttt ttatataaaa caaaattcat atttaatgtg tgttcttgta
     1141 actccttgaa tatctctaat agattgaata tatttaaaaa attatataat ggtatagaat
     1201 ttgatgataa gattatctta agtaaaaatt atagacaagc taaagagatt gtagatttta
     1261 caaataaatt ttcaaataat tctaactcat atatcaacga attaagacca aatacagaat
     1321 ttagtacttt cttttatact aaagcactta gaggtggaaa taagtctgtt gatataataa
     1381 aagtaagtga ccttgatgac caaataagtt ctgtaatctg ggaaattgag tatttaataa
     1441 gtaaaaaagg tcttgattat tctgaagttg ctatcgttta tccttacaat aagaagaaat
     1501 taaaaagtgg aaaaaccatt tactttcaat atatgttaaa aaaagcattg gaagaggcaa
     1561 aaattcctta tatatgtgca gaagataatt tgacaaatat aagtaagaag gtaggaacta
     1621 caatagcaaa tatatatgca ataaagaatt tagagtataa agcggtaata gtatgtgaat
     1681 tagagatgtt atacaatcaa actataaatg atattgaaca agattatcaa gtaaatgact
     1741 ttgtaggaga tttaaataag gtttatttag ctatgagtag agcaacagac tacctaagta
     1801 tagtaacaac ttttaatgaa gaagcaagtg atataataag acttattaca gaatcaaaag
     1861 atatttagta aaaaaatatt tgtaatttaa gaaaataaaa agagactgag atttgtaaaa
     1921 taaactttac caattcagtc tcttattttt tagtctactt tcccaatatt atcaagaggt
     1981 aatagtctca ccataacctt accaagaact tcatcttcat caaccatacc tacatcaggg
     2041 aatctactat cattactatt ttctctatta tcccccattg caaatagttt accttttgga
     2101 acaacagtat ctatatctcc agaagtatag ttattgtgta tatatggttc atttaataat
     2161 tttccattta catacacttt agaatttgat atttttattc tgtcaccttc agtagctata
     2221 actcttttta ttaagtcttt tttctttcct gtttctccat caactaaatt ggttttaaaa
     2281 actacaatat cgcctttttc aggtttacca accttgtatg atatcctatt taaaatcaga
     2341 taatctttat catctaaagt aggatacatt gattctccac ttacaataga aggtcttata
     2401 aattgtagaa ttacaaatgc aagtacaata gccatagcta ttgacttaat ccaatcaaat
     2461 atttcttttt taacactcat aacttttctc cttatcattt gttattaaat tagaatacat
     2521 tataatagaa agaaataagc aagaaccact atacccaaga ctattctata ataaccaaat
     2581 actttgaagt catgtttttt gatatagtcc ataaaaaatt taataactac aattgaaaca
     2641 acaaatgcta ctacagaacc aacgcctaaa ataagccatt caaatccagt aaatccaaat
     2701 ccagctttta gaagttttaa tgcactagct ccaagcattg tagggatggc taagaagaat
     2761 gaaaactctg ctgctacata tctagaagta ccaagtaata ctgcacctat tatagtagaa
     2821 ccagacctag atgtacctgg tataagagct agacactgga ataatcctat acaaagagct
     2881 aatttatatg taacttgaga aaaatcttta tacttaggtc ttttatttct cttttcaagc
     2941 cctatcatta ttataccata tacaataaga gcaatagcaa ctactgttga attaaagaat
     3001 aatctatcta tatcatcttc aaacagtatt ccaataactc cagatggtat aactgcaatt
     3061 ataaccttaa gccataaacg gactgtttct tttttctgct taatattctt tgcgctatca
     3121 aaaggattta attttctaaa aaatataaca agtacagcta atattgaacc aaattgaatt
     3181 acaactaaaa atgtacttat aaatgtattt gaaaaattca attttataaa ctcatcaact
     3241 aatatcatat gccctgtact acttactggt agccattctg ttattccctg aactatgccc
     3301 aaaatgactg ccttaaaaat ttcaagcaaa ctaaatgttt ccaaatcaaa atacctccaa
     3361 tacaaattta tagtagcata ttttattcat agattaataa aaaaatgcca ccttttacta
     3421 acttataata ccatagtact gtgtagtgca caatggaaga ttgatatttt cataaaacct
     3481 tcataatata acaaaataaa cttaaaagtc atctattgaa taaggggtat atttgagggg
     3541 gaaatatatg gtatagtaaa agtatagcta ttttatttta tgaaaaggaa aggagttatt
     3601 atgtatagta caaaaatact agatgattat gagtatgaaa cttttaatgt tttttctaca
     3661 aaattaaatc caaacacgaa acacgattat ttatcaaagg ttatactgtt taaagaattt
     3721 ttaaaaggga aagaattgat ttatgctacg aaagaagatt gtaaaaattt tgtagactat
     3781 atacagacta aatatgcgaa atcaacttgt gaaaagatat atagttattt acatagtttt
     3841 tataacttct taaaaaaaga aggatacata gatataaatc catttagata tgtagaaaaa
     3901 cccacagtaa caagaataaa aacaaaagat gatgttctaa gtatacaaga aataaataaa
     3961 cttataggta tcctaccaaa attaaatata agagatagag taataatagt ctgtctagtt
     4021 actactgggt gtttattaaa tgaattagtt agcttaaagt ggaaagacct tatggtagat
     4081 gaaaacgata actcatatgt aaggttaggt aagggaagaa aagaaagagt agttaaatta
     4141 catccatact tttttaaact tttagaagat tacaggaatt attctgggct tccagaggta
     4201 ataataccta gtgatgattt tatttttaca actcaaaaga gtaattctat aacagataga
     4261 aatgtcagac ttatagtcaa aaaagcatta gatttagctg gactttctca atattcagca
     4321 agagatttta gacattcatt tgcagcaata agtttaaggc ttggggcaga tgaaagtgat
     4381 gtaaaaaatc agcttggatg gagtgataaa tactatgcaa ttagatataa gtatgtatta
     4441 aattttgttg atagtgaaat agtggattac ttaattgaaa aagataattt ggatataaat
     4501 aaaaaatagt aataaataaa aaatactata tataaatgtg aacaaaaaat aaaaaagtct
     4561 tgttatagtt cgtaaaatta tatataatta tttatgtact aatgtgtaca cagtaatcac
     4621 acgacaaaag atttaaggaa ggtgctaata taatgataga taataaatat actacttact
     4681 caaacccact tacagacaga tattgtagca aggacatgag ttatatattt tctccacaat
     4741 ttaaattctc tacatggaga aggttatggg ttgcacttgc agaaggtgaa catgagttag
     4801 gaatcaatat aactgaagaa caattagatg agctaagagc taatatagat aatataaatt
     4861 ttgatgaagc tagaaaaata gaaaaagaag taagacatga tgtaatgtct cacgtaaaag
     4921 cttatggttt acaatgtgaa aaagccaaag gaataataca tttaggagca acatctgcat
     4981 atgttggaga taatacagat gttatacaaa tgaatgaagc tttaaaactt ctaagagtaa
     5041 agcttgtaaa tttaataaac aatttaaaag aatttgcact taagtacaaa gatatgccag
     5101 ctcttggatt tacacacttc caagctgctc aacttacaac agtaggaaaa agagcttgtt
     5161 tatgggctca agatttaatt cttgatttgg aagatttaaa ctatagaata gacaacatga
     5221 agttaagagg agttaaaggt actactggta ctcaagcaag ttttttaagc ttatttgaag
     5281 gagaccacga aaaagtaaaa gagttagata agaaaatttg taaaaagatg ggataccatt
     5341 cttcctatgc agtaagtgga cagacatata ctagaaagtt agattatcaa gtaataagca
     5401 tactatcagg gatagctcaa agtatgcata agatgactaa tgatataaga cttttacaaa
     5461 gtttaaaaga attagaagag ccatttgaaa aaaatcaaat aggttcatca gcaatggctt
     5521 ataagaggaa tccaatgaga agtgagagaa tagcatcttt atctaagtat ataatctctg
     5581 aatctataag ccctgcaatg gtacaagcca ctcaatggtt agagagaaca ttagatgact
     5641 cagccaataa gagattggca atacctcaag catttatggc agcagatgct atacttgaaa
     5701 tagggataaa tgtaactgat ggtttagtag tatatgaaaa tatgataaat agacgtgtaa
     5761 atgaagaact tccttttatg gctacagaaa caatacttat ggaagctgta aaaagaggtg
     5821 gagatagaca ggaattacat gaaataataa gagaatattc tatgaaagct gcctatagag
     5881 taaagcatga aggcaaggat aataacttaa ttgaacttat aataaatgat gattcattta
     5941 aaatgagcaa ggaagaaata ctatctataa tggacccaaa aaactttata ggtagagctc
     6001 cagaacaagt tgtagaattt gtaaatgaag ttgtagaacc tgctataaaa gattataagg
     6061 aagacttagg aaaagtagat gtagacctaa gagtatagaa ttgtacagat aaaatataat
     6121 taaaaacggc tatcttattt ataaataaga tagccatttt aatgggctag cgtcttctct
     6181 ccaccgctgt gaagagttct tctttactta ctataccatg aatttaataa aatagctata
     6241 taaataatat ttaataggaa taatataaat aaaaataaaa aaatgataag attactaatt
     6301 aaatttaaac attcagcaaa aactattgta cttgcaatat ttttaatgta aactgaaata
     6361 aagttattta gataatttaa taaattttaa aacaaccgag gtgattatat gaactatata
     6421 gtaaaagata ccataagttt tttagtaaaa acatttccta agatttattc tagcctgtac
     6481 ttagaagacc taaaaaaatt tgcaccagat tataatgtaa ataaaacaca attaagagca
     6541 ttagttttca taaaaaatta tggagtaata agtatgactg atttatgctc taagttaaat
     6601 atagaaaaag gcagtttaac tagtatggtt gatgatttaa ctgataagaa gtatgtaaca
     6661 aggaaaagag atttagttga tagaagaaaa tatctaattg acattactga agagggagat
     6721 aagatagcaa ctgattttat ggataaatta agtgatggtt taggagaaaa actttctaaa
     6781 ttaactgaag aagacagaaa aaaatattta gaagcaataa atactcttca atatatatta
     6841 aacaaagaag aatttagata gaaaaaatat aaaaa
//
LOCUS       DAELJO010000186         6875 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELJO010000186 DAELJO010000000
VERSION     DAELJO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.188"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1314
                     /locus_tag="KPB88_003206"
     CDS             <1..1314
                     /locus_tag="KPB88_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG0055445.1"
                     /translation="LISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFL
                     ILSINNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFY
                     KNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIY
                     SNGKEIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRE
                     LNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYI
                     SRCVYFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTI
                     IIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGII
                     STRVDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1319..1936
                     /locus_tag="KPB88_003207"
     CDS             1319..1936
                     /locus_tag="KPB88_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055446.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            2125..2469
                     /locus_tag="KPB88_003208"
     CDS             2125..2469
                     /locus_tag="KPB88_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG0055447.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            3039..3905
                     /locus_tag="KPB88_003209"
     CDS             3039..3905
                     /locus_tag="KPB88_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0055448.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            4018..4422
                     /locus_tag="KPB88_003210"
     CDS             4018..4422
                     /locus_tag="KPB88_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG0055449.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLVCGETTVNDGHCHKFLFTTQIEAPLV"
     gene            4598..5047
                     /locus_tag="KPB88_003211"
     CDS             4598..5047
                     /locus_tag="KPB88_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055450.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(5203..5580)
                     /locus_tag="KPB88_003212"
     CDS             complement(5203..5580)
                     /locus_tag="KPB88_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0055451.1"
                     /translation="MLTPYLIFNGDCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            5842..6726
                     /locus_tag="KPB88_003213"
     CDS             5842..6726
                     /locus_tag="KPB88_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG0055452.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEAVKEFWE"
BASE COUNT         2808 a          711 c         1078 g         2278 t
ORIGIN      
        1 ttgatatcta tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta
       61 aatggaaaaa aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt
      121 gtatcatttc ttatattatc cataaataac tgtattttaa attataatat gctacaaact
      181 cagttaggaa atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa
      241 gatacaggcg aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt
      301 ttctataaaa atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa
      361 aaacttgaag atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta
      421 agtcaatatg gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac
      481 tcaaatggca aagaaatatt taatctaatc gattatgacg ataagactat aaatttatta
      541 gttcctaaaa agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt
      601 tattttgaaa aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat
      661 aaaacaaaga agtcagattt aaatgttaat ataatatatg tagataataa tcaaagctat
      721 tttacttata actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca
      781 atagttgaca tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta
      841 tattttaatt ctaagaaatt agatgccctt gcagatataa gtaacattat tgaagctcaa
      901 ggtgtagaag cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata
      961 aataaactag aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca
     1021 aacttaatga ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta
     1081 tatattaaaa aaatatttgg ttattcaact acagctagaa gtatgttgtt aatagtctca
     1141 ttaatattaa caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat
     1201 aatattatct tatttggact tctgatatta gttatagaag taattgtatc tattgtatta
     1261 gacaagatta tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat
     1321 gatttcatta ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa
     1381 cctagaaatt tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac
     1441 aactttactt aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa
     1501 taatataaaa aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta
     1561 tctatttcaa aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc
     1621 tttaaagtat caaaaaaata tcaaccataa tgaaaaaata aattacgcac ttgagtctgt
     1681 aggtcttaga aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag
     1741 ggtagcatta gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac
     1801 aggtaattta gataaaaaaa atagagacat agtatttgat atacttaaaa gcctaaataa
     1861 gaatggtaaa actatcgtat ttgtaactca tgatttagag ttatcggagc aggcagatag
     1921 ggttataaaa ttgtaataat ataaactata ataaatataa gaaagagatt acagctaaat
     1981 caagtttaat aataatacga atagaattta aacattaagt aaattagtag tttgtaaata
     2041 taagtataat atgatatcat aaaggctcaa aagaaatttt atataataag aaatctaaaa
     2101 ataacaaatg gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca
     2161 gaatatactt atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca
     2221 ttaggattgg gagcagaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta
     2281 aatgatggag attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct
     2341 ataaagattg gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat
     2401 gatatagaat gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa
     2461 aaagcgtaat attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca
     2521 taagaatcta ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta
     2581 ttaaatataa attttattga acaccaaaat attctctcct ctacaaataa gccaatctta
     2641 tttaaattaa tatactttgt actatttata ctgaaattgt aaatttaaat actatgttat
     2701 ttaggtttgt atagttaatt tatatatttc tgattttata aattgtattt attattatat
     2761 ctttattcta aaatcattaa aaatttcaac ttctataaag atttacaatt tatcaaatac
     2821 attatgagta tataaacaat ttattattac agtgctacat ttaaaataag tcttagtata
     2881 aaggtattgt tacaagtata aagaaactta tgtatgctat tggaataaat aataccttaa
     2941 attataatta agaataagaa ctgttaaata agggacttga ttatcattta gttatagata
     3001 atataatatt aaaaataaag tttaagagga gaaaaaatat gatagatata cataaaaata
     3061 tatatgataa taagttattt gaggagctaa agattgactg taagaagtgc tttggacttt
     3121 gctgtgtagc tctttatttt tctgcttcag atggatttcc tatcgataag gaatcaggaa
     3181 aaccttgtat taatttacaa ccagatttta aatgttctgt acataatagt cttatgaagc
     3241 gaggttttaa agggtgtact gcatatgatt gttttggttc aggtcaaaaa gtagcacaag
     3301 taacatataa ggggatagac tggatgcaat caccagaact aacaaatcaa atgtctgaag
     3361 tgtttttaat tatgagacaa ttacatgaaa tgttatggta tcttaaagaa gcatctgtat
     3421 taaatatatc agatacaatc aaatcaaaaa ttgatttaat aattgaagaa actgaaaaga
     3481 taactaatat gggcccagaa cagataatta atttggatat catatcacat agaacaaagg
     3541 ttaatttatt attatcacaa gcgagtgagt ctgtgatggg aaaggttaaa tcatttatca
     3601 agacttctac tcttaaaaac atgaaaaaat taagtaaaaa tatagattta ataggagcag
     3661 accttagaaa aataaatcta ataggagcag atttaagagg tagatttcta atagcagcaa
     3721 atttaagaaa tacagatttg agtggggcaa atttaatagg agcagactta agagattgtg
     3781 atattagagg tgctaatctt gaaaatagta tatttttaac acaattacaa gtaaatactg
     3841 caaaaggaga ttctagtacg aagttaccag cttcactaat acgccctaaa tactgggaaa
     3901 aataattaaa ttaatacata attttacaaa atatgaactt ttttgacttt aattcatatt
     3961 atacattaga gattctttac aagaatttct taatataata tagggagaga tataaaaatg
     4021 agttatcaaa attataaatg tgaaagagat tatgtgtatg atgattataa atacaataag
     4081 tgcaacaagt gctataatga ttatgaggaa atgacacatg ttcatgaata ttcagaaagt
     4141 gttaagttag cagaagaatg tgaggataga cataaccacc gtgcagcagg ggttactggt
     4201 gaagttatac caataaatgg aggtacaaat catgttcata aaataaatga taatgtagat
     4261 ttccttgacc attttcataa gatatgtgtt acaacaggac cagcaattag aatacctgga
     4321 actgataaac acattcactt agtatgcgga gaaactactg ttaatgatgg tcactgtcat
     4381 aaattccttt ttactactca aatagaagct cctttagtat aaactaatat gaattattgt
     4441 agtaaatttg ctagattata gtatataaat gttaaaaata tataatagat aatatttaaa
     4501 cttaatgagg actatgatat ttaaccttcg tagtcctttc taagttttgt ataaagaagt
     4561 gttatacaat taaaagtgat atagaaagga aatttttatg aaatctggaa taattcttga
     4621 aatgaaacaa ataaattcaa cagaatatga cgaaatgttg caagtctggg aatcgtcagt
     4681 aagagcaact catgattttt taacagaaaa agatattgaa tctttgatac cattagttga
     4741 aattggactt aaagaagtag aaaatatagt ttgtataaaa gataatgata taataaaggg
     4801 ctttatagga atagataaag ataaaattga gatgctcttt atagaagata aatatagagg
     4861 aaatggtatt ggtaaaaaat taataaaata tgctatagat aaatacaatg taaaatatgt
     4921 tgatgtaaat gagcagaata aaaaagctgt aggattttat attcacttag gatttaaagt
     4981 gtttgatagg tctgaaatag atggacaagg taacccattt ccaatactac atatgaaatt
     5041 aatttaaagt tatacttctc acgtattagc aaaaatgttt acttaaattt ttatacaaat
     5101 aaaagccgtg acagtcttag gacacggctt ttttactaca ctattaaatt gaaatttatc
     5161 tgttctatat tatgattatt agacgtagtt tttttatttt aactatgtac ttaaatacca
     5221 agtatacccg tacctatcaa gtacttcagc agcaccatca ttttgatgtg gtggtgggtg
     5281 tggtaaaaat tcttgtacaa gtgtaccacc ttcggcaagt tttacagata tttcttctat
     5341 cgcttctcga gatggtagaa caacacaaat cgccatgcca gaaattacaa caggcttgtc
     5401 tgtatccgca cccattatac aaccttcgta ttttgtgaaa gtaacacttg cgtgcataac
     5461 aggattgttt ggattgctgt ctaatcgcgc aaatatagtt tttcctcctc cgaaagcctc
     5521 agcataaaaa ttaaatgctt tttcacaatc accattaaat attaaatatg gagttagcat
     5581 gaccaatata tcctccttga tatagaataa tttatagttg cacctgataa tctaaattat
     5641 gatttagtaa attgtatcat agggaaacct tgaagtatat aattttttat tattatatta
     5701 tacaaattta ttaatttaaa aaaatgttat atgtacggaa ggtttacagg ataaaatata
     5761 ataaaaagat taaggtgaca ttataaaaaa tgtatattga cattgacgct acgtaattgt
     5821 atatactttg tcttaaagga gatggtaaat atgaatatta tagaaattcg ctcagataaa
     5881 atatacaaga agataatgga tgcaccaata aacaaaaaag aagatatata cagatatgaa
     5941 ttgatgaagc cttttgaatt taagtggaag tgtatgaatg ttccaatagt tgctagacag
     6001 aaaggtggat atgatgtaat tatagcaagt gaaatgttag gggttttatc gcctaaggat
     6061 attgatgaaa agcaaaaaaa gaatataaat gtgttatctg ctgataaaat ttgggccact
     6121 tgtaaagaaa ccatagaaaa ctctataaat gcttttataa aagaagggta tgatttaaac
     6181 attaaggact ataaatattc aatattattg gcgaatccaa atagtcctta tacaatatta
     6241 agtgatggat actggggtga tggtgggatt cctggatata tatttctatc attggttcct
     6301 aatgaatata ctatcaatag attaccagta ttaatagcac atgaatgtaa tcacaatatt
     6361 agatttcagt ttatagagtg gaataataat ataacattag aagaaatgat gataaatgaa
     6421 ggtcttgcag aaaattttgc aacatggatg tttggagagg aaatgttagg accttgggtc
     6481 agtagaacag atatcgaaac attaaatact tatataaagc caataataaa aagtgcttta
     6541 aaagaaactg gatttcaaaa tataacatct tatctttatg gtgatgatat agctaaaatg
     6601 caaggatatt ttccagtagg gttgccttat tgtgcaggat atgcttgtgg atattatatg
     6661 attaagtatt atttagaaaa gacaaataaa tcaataatcg aagcagtaaa agagttttgg
     6721 gaataaattt tataaattgc ataatttgaa ataaattatg caattatttt atttttaaga
     6781 aaaaataaat tgacaataat ttataagaaa tatacaataa tatatataaa gaaatatttc
     6841 aatatttaaa agttttaaga tttcatatct agagt
//
LOCUS       DAELJO010000187         6856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELJO010000187 DAELJO010000000
VERSION     DAELJO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.189"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..283
                     /locus_tag="KPB88_003214"
     CDS             <1..283
                     /locus_tag="KPB88_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055453.1"
                     /translation="TNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQITQL
                     KNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(376..546)
                     /locus_tag="KPB88_003215"
     CDS             complement(376..546)
                     /locus_tag="KPB88_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055454.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(735..956)
                     /locus_tag="KPB88_003216"
     CDS             complement(735..956)
                     /locus_tag="KPB88_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0055455.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(1040..2266)
                     /locus_tag="KPB88_003217"
     CDS             complement(1040..2266)
                     /locus_tag="KPB88_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0055456.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(2328..3260)
                     /locus_tag="KPB88_003218"
     CDS             complement(2328..3260)
                     /locus_tag="KPB88_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG0055457.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(3349..3876)
                     /gene="hpt"
                     /locus_tag="KPB88_003219"
     CDS             complement(3349..3876)
                     /gene="hpt"
                     /locus_tag="KPB88_003219"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0055458.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            4105..5181
                     /locus_tag="KPB88_003220"
     CDS             4105..5181
                     /locus_tag="KPB88_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0055459.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVKEILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(5267..6475)
                     /locus_tag="KPB88_003221"
     CDS             complement(5267..6475)
                     /locus_tag="KPB88_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0055460.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(6636..>6856)
                     /locus_tag="KPB88_003222"
     CDS             complement(6636..>6856)
                     /locus_tag="KPB88_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG0055461.1"
                     /translation="VLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEI
                     NKYRVIAKKDKHSNIIENVEFSIKDEEE"
BASE COUNT         2340 a         1145 c          727 g         2644 t
ORIGIN      
        1 gacaaacttt aagatatctt atagaaacta tatagaaact ttaataaata ttcaaaatga
       61 aaatttatct aacttaagaa aaaaatctac taaaactgca aaatgtttca atatctctca
      121 aataacccaa ttaaaaaact tactaagtaa tactggttat aatttattaa ttccatataa
      181 atacttaact gctagagata tagaaggaat agttactctt attaatactg ggtctattga
      241 acttggaaat gagagaatct ataattatat atcaaaagca taataattta aacctattat
      301 aaaaaggcaa tttatttaat aaaaaatatt tttaatatta aataaattgc ctttttaatt
      361 ttggattaaa tactgctatc caagtgcttt ttctagttta gataaatgtt gtattggata
      421 atattttagt agctctttaa attgttctac tgataaacct tctgttgttg tatatattct
      481 tattttttca tctaagtcac tattaataaa tttgtttttt aaatcctcaa aatttacact
      541 actcatttta catcactcct tatgtaacat attcatttgt attattttat ccaaatacat
      601 aaaatttatt acataaaaat atatctattt aataatttaa taaaaaaaag ctatctcaat
      661 ttagaaatta ctttctatat ttgagatagc tctagctaaa ctaatagcca atagttataa
      721 ataattattt ataactatct gttgcttttt ccagccattt gttgttcagc catttcaact
      781 aattttttag tcatgtatcc acctacataa ccattttctc ttgcagtaag gtttccttta
      841 tcaacttgtt gataattact cataccaagt tcattagcta tttctaattt catttggttt
      901 aaagcctctt ttgctcctgg tactactgtt ctgttgttgt tattgttact tgccatgttg
      961 attaccttcc ttctgtttaa tttattttgt gttgctgata atattatgtg attttaaaca
     1021 tttttaattc aggtaatttt tagcaattaa aacctgccaa atatccagta gaaaaagcta
     1081 tttgcaaatt gaatccacct gtatatgcat ctatatctat tacttctcca gcaaaaaata
     1141 aaccttctac taattttgat tccattgtac ttgaatttat ttcgtttact tttacaccac
     1201 cagaagttat tatagcttct tcaataggtc tgtatctctt tactgtaaac tttaaatttt
     1261 ttaataaatg gactaagttt tttctctgct ctctagaaat ttggtgaaca accgtatttg
     1321 gattaatttc acttaagttt attattatag gtattagttt ttttggaagt aaatcatcta
     1381 atgctttttc aaatttttta tttgtatact tctgaaaatc tttttgaact cttttatcca
     1441 gtttttcctc atctaatgca ggttttaaat ctaataatat agtgtaattt tctttccttg
     1501 tatctttcat tctacaactt gcagacttga ttataggtcc atctaaacca aatctagtaa
     1561 attcaagttc tccaaaatca ctataaactt tcttttgttt gctgttaaac acccttattt
     1621 caacatttct caatgataat ttttctaaat cttttgtaaa actttcttgt acttctatac
     1681 caattaaaga tggttttgta tctataattg tgtgcccctg tgaaattgca aatttatatc
     1741 catcaccagt agaaccagta agtggataac ttaatccacc agttgctact acaactgaat
     1801 cacacttaat ttctttttta tcgtttaaaa ttactttttc aattttatta tttttagata
     1861 ttattttttc tactttcgaa tttaataata tatttacttt cttactttta agttgtcttt
     1921 caagagcatt tacaatatca aatgctttat cactctctgg aaaaaccctt tttcctcttt
     1981 ctgttttagt ctttactcca agattattaa acatacttat tacatcatca tttgtaaaag
     2041 tataaaaagc actataaaga aattttccat ttgtaggtac attttctatt aattcctcta
     2101 tttcacaagc gttagtaata ttacatctac cttttcctgt tattgcaagt ttttttccta
     2161 atttatgatt tttctcaatt aatgttacat catagccttt ctcacatgca gttgatgcgg
     2221 ctatcatccc tgctggtcca ccacctatta ttactacctt cttcaaataa tcactctttt
     2281 ctacccttaa tttttttaat ttaaactatt gactctatac cttaaaatca atttagtttt
     2341 aaaagtttaa ataatgctgg agctcctgct attccataaa aaacaactac tgtattctta
     2401 ataaaattca taaaaattat actcatagta gagtcactta tatttaaagt gactattaac
     2461 attgaaataa acaacttaat gccaatatat aacattccta ttgttactat cccaaccaaa
     2521 aacctacata ttcttgaaaa aattatattt ttactcccct tatttttatt gctaaaattt
     2581 atttttttgt tattattatc tgtattaaat ttaacaaatc tatcctcaac catatatcca
     2641 aatacaaatc ctactattat accaaatttt tcaaaatatt cagggctatt tagaaatata
     2701 gccactatta taaatggtat aagtaaaact acaagtaaaa tataattttt attatcatca
     2761 atatagtcaa atatttttac aaatacaact gtaaaaaata ttcctaatat ccaacccgct
     2821 atcacatcta ttggccaatg aacagctaaa tacagcctag acaatcctac tcctaaaacc
     2881 ataactgcac ctattaaatg aagatatctc tttttaaata tagtcataat actaacccaa
     2941 aatgttgttg cagtttgagt atgagcactt ggaaaagaat aaccaccagc agtagaaact
     3001 ctcaatgatt caagaccttt tattcctatt ggtctgggag cttttacaaa ctctttgatg
     3061 cctaaattta caacaaaatt tccaattata gcaaacagta ttttctgtcc acatttttta
     3121 tttatacacc agtatataat tgttgtaatt aatattaata acggtgcttc tgtgcttatt
     3181 gtaaagacaa gaaatataaa gttcaaaatt ggatttctta aattttggaa aaataataat
     3241 atatttaact gaatatccat atagatgtct cctttactaa aattttatat aattaactaa
     3301 aaagcactaa ctcccttaga aattagtgct tttgattcac atttaaatct attctacatc
     3361 ctcaactata cctatataag gtaaatttct atacttttca gcatagtcta ttccataacc
     3421 aacaataaat ttgtcttcta taacaaatcc aacatagtct acaggtatat tagcttttct
     3481 tctctgaggt ttgtcaagta gagtacatag ttttaaagat tttggatttc tagtttttaa
     3541 agctgcaact agattactta atgttaaacc agaatctatt atatcctcta caatcaacac
     3601 attctttcct tcaatatcta catctaaatc ttttaatatt ttaactgttc cagaagactc
     3661 tgttgagttt ccataacttg taacactcat aaaatctata tttacatcca aatctataca
     3721 tcttattaaa tctgaaacaa atacactagc tcctttaagt atacctacca ctagtaaatc
     3781 ttcgccttta aagtcttctt ctatcttttt tcctaattca taaacttttt cttttatttg
     3841 ttcttctgtc aacatttttc ctgtaacttt gtacattttg tatcctcccg ctttccttct
     3901 attaacttat atattatagc ataccaaaat atcaattaaa atctttttca tcatttttaa
     3961 acctaaatta acagcttatc catacttttt tgctttttac attaaaaatt tggaataacg
     4021 attcctttgt attataatct atatgtacat atattaatca gttattttta tcgattatat
     4081 aattttattt ttggaggtac ttttatgagc acaaaccatg gagctaattt atatagttta
     4141 tctagtaagt atggcttttc aaaggaagaa tttatggatt ttagctcaaa tataaatcct
     4201 tttggtacat caagtttagc caaacaatac attgtaaaca atatagatat ggtatctatg
     4261 tatcctgacc ctgattacat agacttaaaa acttctatat ccaattactg caaatgttct
     4321 atagataata tagtacttgg cagtggagct acagaactaa tttcatcatt tatacataca
     4381 attaatccta aacaagctct tttactctct ccagcctatt cagagtatga aaaagagtta
     4441 tctaaaatta attgttctat agaaaaatac tttgcaaaag aagaagataa ttttcatata
     4501 aatttagaaa atcttataaa aactatcaat gctaaagatt atgatttggt agtaatctgt
     4561 aatcctagta atccaactgg atttgcattt actaaagtag aagtgaaaga aattttaaaa
     4621 aatacagact catttttaat gattgatgag acttatgtag agtttactga tacagataca
     4681 tactcttgta ctcaattagt tgatgattat tctaatttat tcgttataag aggtacatca
     4741 aaattttttt caacgcctgg aataaggctt ggatatggtc ttatatctaa cactaatgtc
     4801 aaaaatgaaa taaataaaaa tctagattta tggaatataa acataatagc ttctaaaatg
     4861 ggtgaaatca tgtttagtga tttagatttt atatcaaata ctatttcctt aatgaacact
     4921 gaaagagatt acctgcttaa agagcttaag aatataaaaa gcttagatat atacaatact
     4981 aaaggtaatt tcatattgtg taaaatcaag acaaaggaat tgactgctaa atctcttcga
     5041 gagcaactgc ttccacaaaa aataataata agagattgtt gttcatttga aggtctagat
     5101 gagtatttct ttagagtatg tatattaaaa cctaatgaaa ataaattatt aatatcatct
     5161 cttaaagcaa tttttaaata aaaaaatcac tttattttat aattttttat accataaaaa
     5221 atgagctatc tctatttgag ataactcatt tttgattatt aaaaaactaa gcactttgct
     5281 cttctttatt tcctttttta ctcattccat catttatttt atctactact actgctattg
     5341 cattatctcc tgatacatta catgctgtac caaaactgtc ttgagttaag tataaagcta
     5401 tcattaaact tgcaagccca ccatctgatg gtattccaac cattggtagg aaaggaagtg
     5461 cactcattat agccccacct ggtgctcctg gtgctgcaac cattgctata cctaacattg
     5521 caatataagg gaataataca cctattccat gtggcatacc attcatcatt aaaactgcag
     5581 ttgtaaatcc tgttattgat atcatactac ctgataaatg tattgttgca caaagaggta
     5641 caacaaattc tcttatttgc ttacttactc catttttttc tgcacaacca acattaactg
     5701 gtatagttgc tgctgaagat tgagttccta tagctgttaa atatccaggt acttgatttt
     5761 ttaacattct tactggattt tttcctgaaa atacacctgc tgctgtgaat tgacatgcca
     5821 tatatacaaa atgtaaaggt attactacta agtatacttt ccagaatacc cctaatattg
     5881 tccatacttc ccctgctata gttatatttg caaaagttcc tgctatgtat aaaggtaata
     5941 aaggtataat cactgttgac aacgttttcg ttacgattcc ttgaaattct tggaagaaat
     6001 taaataaagt ctctcctttt ccatgatttc ttaaagctga tattccaagt cccattacaa
     6061 atgcaaatac gattgcagct gttacatcta ccataggctc taatggaatt gtgaatattg
     6121 gagctaacat tcctgcctct gggtctccaa tttgacttgc aacacttgca tcaataaaat
     6181 ttgggaagat taatgtagat actgcaaatg ataataatcc tgctattaag gtagaaccat
     6241 atgctattcc cgtagttatc cccaataact ttcctgcacc tgttgctaaa tcagctatac
     6301 ctgctgttac aaaacctata attataaatg gtataacaaa ttttaggaat gctgagaata
     6361 aagtactaac tgttactaat atttgtacta ttatttctgg tgcataactt ccgattaaaa
     6421 taccgactat gataccgatg attagtttgg gcactaaacc tatttttttc ttcataatat
     6481 ttaccctcca taactttttt aaattaagca ttcttgcaca ttgaatactt agttaaatat
     6541 tggatatcca cttattatga taatagagtt ttacctctcc gtcaacaatt taaccatttt
     6601 ttgttaataa aaaaaagaat aacatatata aattgttatt cttcttcatc ttttatagaa
     6661 aattctacat tttcaattat atttgaatgt ttatcttttt ttgcgataac tctatactta
     6721 ttaatttctt cattcactat acctatactc caaattaaaa ctatagcatc atctataaaa
     6781 ccaaatgccc ctgcaatcat ctctggaatt atatcaaccc cagatacaaa gtaggatata
     6841 gtaaaaatta atacag
//
LOCUS       DAELJO010000188         6853 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELJO010000188 DAELJO010000000
VERSION     DAELJO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.190"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..931
                     /locus_tag="KPB88_003223"
     CDS             <1..931
                     /locus_tag="KPB88_003223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055462.1"
                     /translation="KKIQNELPRFLQSKGFNIKRGHEHSKARHMQPEDYKKQQIQELN
                     ELENKLEYVKKLFKGFEKPLNSFDELEPANVKKSLITGKETVYKNDYDEVIDILKGTM
                     KENLELKEKLHNEQLKTRNLEVKLSTVNEYERLTYEKEKKLKLEKEEFEEQKQLISER
                     EKNIEKVLENKDKNNKKILDEKEIEINELEKQYKNLSRKYNNTIREYEKVIEEFSNND
                     IYIIFNKSSIKEIQENDTYRFKDANSLIKVLNERYFNSNETKEIGFNMSFGANNENVF
                     LQKNIILGQYIDIDLRQKIIISNRKHTLISRDI"
     gene            1039..1407
                     /locus_tag="KPB88_003224"
     CDS             1039..1407
                     /locus_tag="KPB88_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0055463.1"
                     /translation="MKKIPKTEHMVMNFIWSQNKSKVSSKDIANYMAEEYNWFKGTTG
                     KVLSRLSDKGFLEPEKEGRNTIYNVKINRDEYIKFETEEFFSFVHHNSIHSFISALGE
                     GEDISDEDIKELEDWIKNRG"
     gene            1415..2677
                     /locus_tag="KPB88_003225"
     CDS             1415..2677
                     /locus_tag="KPB88_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0055464.1"
                     /translation="MNIFIEIIKLNMYMSLPIILFILFNKKLLVKYTAMFSYTLCLGI
                     ILRMMIVSKVNLNIPIIDNENIALSRKSYIFSDYIFENNFLKILFYIWIIGVVLISLY
                     YIYCHIKFCNYITNLSKEILDTSIYNIFNDQKENLNIKKKFKLYVVEGIYSPAITGLR
                     KNKIIIPNKKFKEKELKLIFRHELIHYKRKDNFLKLIMLIVNILYWFNPICYLLKKHF
                     NELCELSCDELVIKGYNIDEVKDYAYVLLDTIKYKNKLKQSICISQFYTEKNSIIKRR
                     LDRILNVKSQKRGLNLGLLISLVIIGSIFTFNLNINAQNIIISNNENGNIRYNKNGET
                     YGTSIINKDGDIIEEPNLIYAQGENNVKGYVKKNDLYDEENQPQNPKEAVAYMKEKRK
                     LSLNPFYKNTIPIYDKDGITILGEFKID"
     gene            complement(2773..3531)
                     /locus_tag="KPB88_003226"
     CDS             complement(2773..3531)
                     /locus_tag="KPB88_003226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055465.1"
                     /translation="MIKKSKLSKALAISLAAASLFSNNAFADTNRPNLNIKEYSNVSM
                     DRKSTSNTTSFSANGKNYYNYTSVEGTYAGAYGSTVIKCTSGKVTDGIMGVHANLYNS
                     SGNVVKTTNWQYNTRTTDFHSASTKMHNVKGTYHTQGTTKAYNGSGYKTVGAKKSPIA
                     TVKSLNIDISEQELKERQYLYETKNLISAVGFNDIKGYVSLDDLYDEENQPQNPKEAV
                     AYMQRLERSNIKYKSIPLYDKDGETIIGEYHIDL"
     gene            complement(3893..4486)
                     /locus_tag="KPB88_003227"
     CDS             complement(3893..4486)
                     /locus_tag="KPB88_003227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055466.1"
                     /translation="MNKDKFNELDISEQIEYINNQLENNETLTYICKNIGIGRSTIRD
                     RFKKANYSYSKDLNKYIHECITDVLQDPSIKNNRCITTDIQKEKNNISNTNVIQSDAV
                     KEVINKSDKEIKDNLLDIVSNYDILKEIIELHKCNTSVIKQQIVIDLEDSDSKLTTLR
                     VNSKELELFNKFCDNNKQFKKMDLISQALKEFREKYK"
     gene            5504..5860
                     /locus_tag="KPB88_003228"
     CDS             5504..5860
                     /locus_tag="KPB88_003228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055467.1"
                     /translation="MKDYLIENTTKEQRREFVAGALGIASIDSHEPSEIILKLSDRYI
                     EGELEIEEIRDIVLSFYKRRYLDGDESIETLEDYVLILEKEKRRNNIVTDEEGIEKIM
                     DKIIDENLDALKKLSE"
     gene            5875..6099
                     /locus_tag="KPB88_003229"
     CDS             5875..6099
                     /locus_tag="KPB88_003229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055468.1"
                     /translation="MYIVAKKFGEVGCIASKVERGERVGALIEYLSLTMLDKGIEIVV
                     LDKPNMYGEYAPYEFLSEVEFVCSVLSMNH"
     gene            6384..>6853
                     /locus_tag="KPB88_003230"
     CDS             6384..>6853
                     /locus_tag="KPB88_003230"
                     /inference="COORDINATES: protein motif:HMM:NF013258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBG0055469.1"
                     /translation="MAFLVCHVAKYSRNNIVGIQKHNQRQNKSYKNKDIDITKSNKNI
                     DFENKTRIKYLEKVEKIIKENRTKGKVIRKDAILMCENVISASPEFFLGMEQSEKEKY
                     FKYAYEFLKDRYGDKNIVSATVHFDETTPHMHFNFVPITSDGKLSARDLITRIEL"
BASE COUNT         2720 a          713 c         1042 g         2378 t
ORIGIN      
        1 aaaaaaaata caaaatgaat taccaagatt cttacaatca aaaggattta atataaaaag
       61 aggacatgag catagcaaag caagacacat gcaaccagaa gattataaaa aacaacaaat
      121 tcaagaactt aacgaattag aaaataaatt agagtatgta aaaaaactat ttaaaggctt
      181 tgagaagcct ttaaatagtt ttgatgagtt agaacctgct aatgtcaaaa aaagccttat
      241 aacaggcaaa gaaacggtgt acaagaatga ttatgatgaa gtaatagata tactaaaagg
      301 aacaatgaaa gaaaatctag aacttaaaga aaaattacat aatgagcagc tgaaaacaag
      361 aaatttagaa gttaagttaa gtacagtaaa tgaatatgaa agattgacct atgaaaaaga
      421 aaaaaaatta aaattggaaa aagaagaatt tgaagaacaa aaacaactaa tatctgaacg
      481 tgaaaaaaat attgagaaag tactagaaaa caaagataaa aataataaga agattcttga
      541 tgaaaaggaa atagagataa atgagttaga aaaacaatat aaaaatttat caagaaaata
      601 caataacaca ataagagaat atgaaaaagt gattgaagaa tttagtaaca atgatatata
      661 tattattttt aataagtcta gtatcaaaga gatacaagaa aatgatacat ataggtttaa
      721 agatgccaat agtctgatta aggtattaaa tgaacgttat ttcaatagta atgaaacaaa
      781 agaaatagga tttaatatgt catttggagc taataacgaa aatgtctttt tacaaaaaaa
      841 tattattctt ggacaatata tagatataga tttaagacag aagattatta tttctaatag
      901 aaaacataca ttaatatcta gagatatttg aaaaaaattt agactactta taaactctat
      961 ttaaataaaa gaaattaaaa aaattgactg ttagacaaat gtctagtata ataaaaataa
     1021 aggttggtga tttgtattat gaaaaaaata cctaaaactg aacatatggt aatgaatttt
     1081 atttggagtc agaacaaatc taaagtaagt tcaaaggata tagcaaatta tatggctgaa
     1141 gaatataatt ggttcaaagg tacgacagga aaagttcttt caagattaag tgataaaggt
     1201 tttttagaac cagaaaaaga aggtagaaat actatttata atgtgaaaat taatagagat
     1261 gaatatataa aatttgaaac tgaagaattt tttagttttg ttcatcataa ttctatacat
     1321 agttttatat cagctcttgg ggaaggtgaa gatatctcag atgaagatat aaaagaactt
     1381 gaagattgga taaaaaatag gggatgatgt aagtatgaat atatttattg aaataataaa
     1441 attaaatatg tatatgagtt tacctattat tttatttatt ttatttaata agaaattatt
     1501 agttaaatat acagctatgt tttcatatac tttatgtcta ggaataattt taagaatgat
     1561 gattgttagt aaagtaaatt taaacatacc tattattgat aatgagaata tagctttaag
     1621 tagaaaaagt tatatcttta gtgattatat atttgaaaat aattttttga aaatattatt
     1681 ttatatatgg ataataggag ttgttttgat atcactatac tatatctatt gccatataaa
     1741 attttgtaat tatataacaa atttaagtaa ggaaatatta gatactagta tatataatat
     1801 ttttaatgac caaaaagaaa atttaaatat aaaaaagaaa tttaaactat atgtggttga
     1861 aggaatctat tctcccgcta ttactggatt aagaaagaat aaaataataa ttcctaataa
     1921 aaaatttaaa gaaaaagaat taaaactaat ttttaggcat gaattaattc attataaacg
     1981 aaaagataat ttcttaaaat taattatgct tattgttaat atactgtatt ggtttaatcc
     2041 aatttgttac ttattaaaaa aacatttcaa tgagttatgt gagctatctt gtgatgaact
     2101 tgtaatcaaa ggatataata ttgatgaagt taaggattat gcatatgttt tgctagacac
     2161 aataaaatat aaaaataaac ttaaacaatc aatatgtata tcacagtttt atactgaaaa
     2221 aaatagtata attaaaaggc gcttagatag aattttaaat gttaaatctc agaaaagagg
     2281 cttaaatctg ggtttattaa tatccttggt aataatagga tctatattta catttaattt
     2341 aaatataaat gcacaaaata taattattag taataatgag aatggaaata ttagatataa
     2401 taaaaatggg gaaacttatg gaacatctat tattaacaaa gatggtgata taattgaaga
     2461 accaaatttg atatatgctc aaggcgaaaa taatgttaaa gggtatgtga agaagaatga
     2521 tttatatgat gaagaaaatc aaccacaaaa tcctaaagaa gcagttgcgt atatgaaaga
     2581 gaaaagaaaa ttaagcttaa atccatttta taaaaataca ataccaattt atgataaaga
     2641 tggtataact atattaggag aatttaaaat tgattaaaaa atgagttata atatttaaat
     2701 aaaataaaac actagtctaa ataattattt agactagtgt tttattttat ttaaattaag
     2761 ttatgtagca tattaaagat ctatatgata ttcaccaata atagtttcac catctttatc
     2821 gtaaagaggt atgcttttat atttaatatt tgatctttct aatctttgca tatatgcaac
     2881 tgcttcttta ggattttgtg gttgattttc ttcatcatat aaatcatcaa gcgatacgta
     2941 tcctttgata tcattaaagc ctacagctga aattaaattt ttagtttcat atagatattg
     3001 acgttctttt aattcttgct ctgatatatc aatatttaag cttttaacag ttgcaatagg
     3061 gcttttttta gcacctactg ttttataacc gctaccatta tatgctttag tagtaccttg
     3121 tgtatgataa gttcctttta cattgtgcat tttagttgaa gctgaatgga aatcagttgt
     3181 tctagtattg tattgccaat tagttgtttt aacaacattt ccacttgaat tatataagtt
     3241 tgcatgtact cccattattc catctgtaac ttttccagaa gtacatttta tgactgtact
     3301 cccatatgca cctgcataag taccttcaac tgaagtatag ttataataat ttttaccatt
     3361 agcagaaaat gatgttgtat tactagttga ttttctatcc atagacacat tactgtattc
     3421 tttaatattt aagttgggtc tattcgtgtc agcaaatgca ttattagaaa acaatgatgc
     3481 tgcggctaga gatattgcta gagctttgct aagtttactt ttttttatca taaataatta
     3541 ccccttaaaa attatattag acaaaagtct tatttatcaa taattgtatt agacaaaagt
     3601 cttaatgtca atatatttat agaaatattt tctatttaga aattaaaaaa taattattgg
     3661 taagtagaat gtatagtaaa aaaactaatc cttaaattga cgtttaggga ttagttggat
     3721 ttatttcagt gttttcaata cctataaaaa taaaatagta ctaacttaaa ttcattaagt
     3781 tagtactatt tttaatttct tattataatt tggataaata atttaatata ttcgattatt
     3841 aactcttatt tctatttttt actttatttt gtttgattta gcctaatgag ttttatttat
     3901 atttttctct aaactctttt aatgcttggc ttattaaatc catctttttg aattgtttat
     3961 tattatcaca gaacttatta aataactcta attctttact attaactctt agagtagtaa
     4021 gtttactgtc agaatcctct aaatctatta ctatctgttg ctttataacg gacgtattac
     4081 atttgtgtaa ctctataatt tcttttaata tatcataatt agaaactata tctaacaaat
     4141 tatcttttat ctctttgtca gacttattaa ttacttcttt tactgcatcc gattgtataa
     4201 catttgtatt actaatgtta tttttttctt tttgtatatc agtcgtaata catctgttat
     4261 tttttatgct tggatcttgt aatacatctg ttatacattc atgtatatac ttgtttaagt
     4321 ctttggaata actgtaatta gcctttttaa atctatctct tattgtactt ctgcctattc
     4381 ctatattttt acatatatat gtcaaggttt cattattttc taattgatta tttatatatt
     4441 ctatttgttc tgatatatct aattcattaa atttatcttt gttcatacga cacccccaca
     4501 gtaataacag atgtattacg tctgttattt gaaattatat cataatatta tttttaataa
     4561 atttggagtt ttaaaatata attagcaaaa atttcaaaaa atatgttgtt atatcctcag
     4621 aaaaaattta tacttaaata tataaaaact aaataaaaaa gcacttacaa aaagtaagtg
     4681 ccttctgtaa cttcagttct ttaaatcgcc taggaaacga tttaaggtaa caactggtat
     4741 agaaagtacc aagaagttaa ggaataatcc aatcgcaaga taggctaaat ctatcaagga
     4801 gagttgaccc aaccgaaatt atttacaaaa tataattata gtatataaag taaaataatt
     4861 ttagtcaagc ggaaatgttg aacacctaaa gtatagaagt ctatacttta ggtgtttttt
     4921 aatgcaataa atgaggttgt atgttaccta tatgacatat agtgggacag cccttaacca
     4981 ctcaaaaaaa aggtcaggtg agttcagcca atgcagaaag tgaacctctg tacaatatag
     5041 ctaatccctt tttatagcta gtacaggatt gagtggtgac gacaacctaa aggtcgggtg
     5101 tgggagttct aggagcataa gtgcttggga actatgaaca gaattattgc attaggatag
     5161 aaccataatg tagaaagttt ataggtgttt acacgtaata aaaagggtac agatgataag
     5221 gtgaccgaag tatacggttt atagatttgt aattacttcc ttatgatagt caatttaaaa
     5281 gtaatgacta ttataaggga ataattacgt ccaatttcag ttccctcgtg ccggtttatt
     5341 attttagtga acttgtcaag ataaaattta aataggaata acaaaattct ccatatgcca
     5401 agccccttct ggcttggcat atggagaatg cgttagtaac cacttgaagc caaaggttaa
     5461 agtggtttta gccgttaggc ttaacaaaag gaaggtggag attatgaaag attatttaat
     5521 tgaaaataca actaaagagc aaagaagaga atttgttgct ggtgcgcttg gaatagcctc
     5581 aattgattct catgaaccta gtgaaattat tttaaagtta tcggatagat atattgaagg
     5641 agaattggag atagaagaaa tccgagatat agtgcttagt ttttataaaa gaaggtatct
     5701 tgatggagat gagtctatag aaactttaga agattatgtc ttgatacttg aaaaagaaaa
     5761 aagaaggaat aatatagtga ctgatgaaga aggtattgaa aaaataatgg ataaaataat
     5821 agatgaaaat ttagatgcct tgaaaaaatt atctgagtga tatgaggtga agtaatgtac
     5881 attgttgcaa aaaaatttgg tgaagtaggt tgtattgcat caaaggtaga aagaggagaa
     5941 agagtaggtg cattgattga atacttatca ttaacaatgc ttgataaggg gattgaaatt
     6001 gttgttttag ataagccaaa tatgtatgga gaatatgctc catatgagtt tttgagcgaa
     6061 gttgagtttg tatgtagtgt tttatcaatg aaccattagt ggtctattta gaccactagg
     6121 gtgtgccaaa agtgaccact tttggtatat agggttttaa tgcctcgcag agcttaccac
     6181 tttgccaaca tagtgtaaag tatagtaagt tagacttata tgtataaata tgctattctt
     6241 gattataaat aatcaagaat ttctataaca tgactatagt catgttatag aaggcttatt
     6301 tgtatgtata agtctttttt ctattaaatt acaaaacttt aaaaatgaat attgagattt
     6361 tctaattaga agggagttta agtatggcat ttcttgtttg tcatgtagca aagtattcga
     6421 gaaataatat agttggtatt caaaaacata atcaaagaca gaataaatct tataaaaata
     6481 aagatattga tataacaaag agtaacaaaa atatagattt tgaaaataaa actagaataa
     6541 aatatttaga aaaagtagaa aaaattataa aagagaatag gacaaaggga aaagttataa
     6601 gaaaagatgc aatattaatg tgtgagaacg taatttcagc aagtccagaa ttttttttag
     6661 gtatggagca atcagaaaaa gaaaaatatt ttaaatatgc atatgagttt ttgaaagata
     6721 gatatggtga taaaaatata gtttctgcta cagtacattt tgatgaaacg acacctcaca
     6781 tgcattttaa ttttgttcca ataacatcag atgggaaact aagtgctaga gatttaataa
     6841 caagaattga act
//
LOCUS       DAELJO010000189         6826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELJO010000189 DAELJO010000000
VERSION     DAELJO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.191"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..602
                     /locus_tag="KPB88_003231"
     CDS             <1..602
                     /locus_tag="KPB88_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055470.1"
                     /translation="IIKLILEQNENLIDEKISDDIDSEIFRIENSVEKILYLSRLEDF
                     HKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLNFKVLSDEKWLYFIFEQLLS
                     NSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKS
                     TGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
     gene            714..1943
                     /locus_tag="KPB88_003232"
     CDS             714..1943
                     /locus_tag="KPB88_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0055471.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1951..2625
                     /locus_tag="KPB88_003233"
     CDS             1951..2625
                     /locus_tag="KPB88_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055472.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2628..3860
                     /locus_tag="KPB88_003234"
     CDS             2628..3860
                     /locus_tag="KPB88_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055473.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(4146..5411)
                     /locus_tag="KPB88_003235"
     CDS             complement(4146..5411)
                     /locus_tag="KPB88_003235"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG0055474.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSSVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5464..6612)
                     /locus_tag="KPB88_003236"
     CDS             complement(5464..6612)
                     /locus_tag="KPB88_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0055475.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6712..>6826)
                     /locus_tag="KPB88_003237"
     CDS             complement(6712..>6826)
                     /locus_tag="KPB88_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0055476.1"
                     /translation="AKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRHFGK"
BASE COUNT         2406 a         1067 c          938 g         2415 t
ORIGIN      
        1 ctataataaa actcattctt gaacaaaatg aaaatttaat tgatgaaaaa atttcagatg
       61 acatagatag tgaaatattt agaattgaaa actccgttga aaaaatttta tatttatcaa
      121 gactcgaaga ttttcataag gattttttaa ttcaagaggt aaatattgaa aaaatcatta
      181 gaaatattat aaaaaaatac tcaaaatatt ttatttcaaa aaaaattatt ttagacttaa
      241 ataatttgaa ttttaaggtt ttatctgatg aaaaatggct atacttcata ttcgagcaat
      301 tattgagtaa ttccctaaaa tactcctcat taggagataa tatatctatc tattgcaaaa
      361 gaaccaaaaa ttatttacaa ttaagagtgg aagatactgg ttgtggtata aaaaaggaag
      421 atttaaatcg aatattcaat aagggattta caggtaataa tggaagaaac aattctaaat
      481 ctacaggtct tgggctttat ttagtaaaag aactttgtga taagcttggt cataaaattg
      541 atgtagattc agagtacaac caatatacaa aatttactat tacattcaaa tgtgatttat
      601 agtatcttat tattgatagt ttattttcat taacttatat tcaacttctt atagtaattc
      661 tatatgtaca tcccagtata taattaagta gtttttataa aaaggaggaa gacatgtctt
      721 ttctaaaaaa atttaaaaat aaaagattta gaaataacag ttttaaaagt aacaacttta
      781 gaaatagcag ttttggaagt agtggcttca agtctaaaaa taaaggtttt aaaaacaatc
      841 cctttaaaaa taagagattc aaaatatatg caggaatagc agtaatcata cttttaattc
      901 taggagttct tgcttatgtt gattcgaaaa acactgaatt acaagctaat gaaaatttta
      961 tcgaaacata tactattcct gaaaatgaaa aaatatttat aaatgggatg gttgttccta
     1021 agcaaactaa ggacttcaat atatctggag attatgagtt aagtgatgtt aatgttacaa
     1081 atggtcaaaa agtaaatcaa ggagaccttc tttttactgc taaaaatcca actataatag
     1141 cagaaataga tagtttaaaa tcacaactta gtcaatacaa aaaacaaaaa atatcacttt
     1201 ctgatattgc tgaaaataaa gatgctattg cttctataaa tgcacagata actgctttaa
     1261 actctcagat agcttctctg gataaaaaag cttatgacag agttactgct ccttttgatg
     1321 gcacagtata cttgaatgac caaactggaa atccagacca accagtttca tttatgacta
     1381 ttcaaggtct agagttttat atgaaaggac aagcaagtga acaagattta cctaaaatga
     1441 agattgacca aatggtcaat atactagttt tttcaactga ccaaaagctt actggtagaa
     1501 taagttttat atctgataaa ccatctactc caaatactga aatgggagct cagcagaata
     1561 cactatctta ttatgatata aacatagcat ttgataatca agaaggatta gtaaatggat
     1621 tccatcttca agcatcactt gaagtagcta attcaagctt taaaatacca gcatcttgtg
     1681 tacttaaaga caacaaacac tcatatgtat ttaaggattt agatggtata ttgaaaaagc
     1741 aaattgtaga tgtagcaagt caaaatgatg attttgctgt agttagaggc ggtcttgaac
     1801 aaggcgatat aataataaaa caccctacta aagaaatgaa agaaggagac ccagttcaag
     1861 gcgatggcgt aactgctggt tctaataatg gagatactac tccaaacaaa aaagtagaag
     1921 aagttactat ggatgtgaat taatttacat atgttaatta aattagaaaa tattcaaaaa
     1981 tattacaaag tagggaaaga tgaattacat gttttaaaat ccttaaattt agaaattgaa
     2041 tctggagaat ttgtcatgat aatgggtaag tctggtagtg gtaaaactac tttacttaat
     2101 atcttaggtt ttctagatgt atttgatgaa ggaagatata tatttgatgg aacagatgta
     2161 actaatttga gtgaaaatga acgttctgtt tttagaaata ttaatattgg atttgttttc
     2221 caacaattta atttaataga aactctaaat gtatatcaaa atgtagagtt gcctctaatt
     2281 tacaataaag cactaaaaaa atctaataga gaagaaattg ttaaagataa attaagttct
     2341 gttgggcttt tggacaagct taagcagaaa ccacttcaat tatctggtgg tcaacaacag
     2401 cgtgtagcca ttgcacgttg cctagcaaat gaccctcaga taatctttgc tgacgaaccg
     2461 actggggcat tagatagtga aactagcaga gaaattatgg agcttttaac aaggctcaac
     2521 aaacaaggta agactataat aatggttaca catgatcaag atttaactaa gtatgcaact
     2581 aaggttatac gacttaaaga tggtgtgttt acttcggagg tgtaatcatg agtttattaa
     2641 aaaattcttt ggccaattta aaaggtcaca agcttcgtgt atttgtagct ttactctgga
     2701 ttataatagg aataacttca gtaatacttg ttagttctat aggtaatgga tttcaaaaag
     2761 aaattaagaa atctgtaaac aatgtaaacc ctaataaaac tactatttcg tttgaatcag
     2821 ccgataacac aggtcttact gatgatatga gtattttttt aaaacccttt aatgcaaaag
     2881 atttagaaga attatcattt gttgaaggag tagaaagaat agctccttca agagatgggt
     2941 ttaaccttga ttcagtttac tcttcacaag catcttttga taaaaaaact acgtatgttg
     3001 atgttggacc agtaaaaaaa gattctaaaa taaatttaat ttgtggtaga gatttttctt
     3061 tagatgatga aaaaagaaaa gtaattttac ttacattaca aagtacaagt gaaatttttg
     3121 aaaatccaga agatgcctta ggtcatggta tcaacataaa tggaactata tttgaaataa
     3181 taggggtatt agatgattct cagcaaaacc aagctggagg attctttgga ggttaccaag
     3241 atatgcaatt tactacttcc ctagtaccta aaaaggcttt tgacacttta atgagtcaaa
     3301 attcttattc aaatgaaatt taccagttag acttagtttc ttctaaaggc tataatgtaa
     3361 atgaagttgc aaacaatgtt atagctaaat tatatgagat gcaccctggt atcaatggtt
     3421 cttatactac accagaccca actgaacaga cagcttatct tgaaagcatt aattccaatg
     3481 taaacaaata tgtatctata ataactgtag ttgcaatgtt tgttggtggt attggtgtaa
     3541 tgaatataat gtatgtatct gttatggaaa gacaaagaga aattggtata agacgtgcta
     3601 ttggagcaaa acctaggtct atattatttc aatttttagt tgaagctgtt tttataacag
     3661 tatgtggtgg aattttagga actatagttg gatttactgc tactaactat gtttctaaat
     3721 atataggttt tgaagctata cctagcttaa atagcttatt ttatgcaatt gtagctacta
     3781 tattgactgg agttgtattt ggtctgattc cagcctttaa agcctctaaa ctagacccta
     3841 taaaggcaat ttataagtag aaattgtata aagattttcg atttttagat tagtttatat
     3901 aaaacatact taaatatata acgcactatt atatatgata gattaggaac ttaatctttc
     3961 caaatataat agtgcgttct ttcataaaaa ggattctctt ccttactata tctaaattac
     4021 caagatgtta tgttctaatt gttcaaacta atacttccaa ttttgattaa tcaatctcat
     4081 tctcctaata tacaatcttt tactttcaat cattgtacac tccgccttaa aaaaataatc
     4141 ttaaattagg cgttatatac aatctcccca ttacatattg tataattaac ctttccatat
     4201 aaagatttac ctttaaatgg tgaattatca gctttagaaa caaagctttc aactgtccat
     4261 tcttcttctg gattgaatat aacaatgtct gctacagccc cttcttctat aaatccaata
     4321 tttaaattgt acaactttgc aggatttata gacatttttt ctattaactg catcatacta
     4381 agatgatttt tatgcaccaa atttgttatt ccaagagcta aagaagtttc aagcccaata
     4441 attccacttg gagcttttat aaactctcta tctttttctt ctttactatg aggtgcatgg
     4501 tccgttgcta ttatttcaat agtaccatca tttaatccct ctataatctt atctctgtcc
     4561 catttacttc ttagaggtgg attcattttt gcatttgttc caaactccaa tacatcttcc
     4621 tcagtcaatg taaagtgatg tggtgaagct tctgcaacta catttgcacc taagtatttt
     4681 gcaaatctaa ccatatcaac agatacactt gaacttatat gttgtatatc aactttagca
     4741 ccagtcttta gtgccaacat acaatctctg gctaccataa catcttctgc tacacttgat
     4801 gcacctttta atcctagttc tgaagctacc tttcctgcat ttacacctgg atttccaact
     4861 aaagaagggt cttcttcatg aaaactaagt ggtacatcca actcttttgc cttaatcatt
     4921 gcctccataa ttagattgct atccattaaa ggtaatccat catcagtaaa ccctggtaca
     4981 ccagcttttt taagagcttc catatcaact aactctttac cttcaaaacc tttagttata
     5041 gctgctgctt gtaaaacatt tatacatgca ttttttgact tttctttaac ataattaaaa
     5101 gtatcttcat tatctacaat aggattagta tttgccatac atataacagt tgtaaatcca
     5161 cctctagcag cagatttagc tccactttct atatcttctt tatatgtaaa cccagggtct
     5221 ctaaagtgta catgtacatc cactaaacct ggagcaacta cattaccact agcatctatt
     5281 attttttcat attcatcact ttcatcaaac ttgcctattt tataaatttt attttctttt
     5341 ataattaagt caatattctc atctctttga cttttagggt ctattactct accattttta
     5401 attaaaatca tattatttct cctttatatt tttaatttat atagactcat ataatattat
     5461 acattatttt acatacttaa atacaaaatt acgtttgaca tcaatagcct ttttaggaca
     5521 aagttcatgg caacagtaac atcttataca tttacttaag tctataatag cacccttagt
     5581 ccctatctct atagtggatg caggacaagc ttccttgcat tttccacatt ttatgcaatc
     5641 ttgaaatctt accacaggct ttggtgttat aagctttgaa ataggttcat ttaaaaattt
     5701 tggtagcttt acggaaaaca atcttaaatc cttacttaca tttggaatct tgtaattttt
     5761 tataattaaa tcttttatat cttcacctaa aatttcaata tctgaaaaat cttcttgtat
     5821 atatcctctc ttaatagcac ctctaattgt agcaactcta tttggaagta aacttataat
     5881 cttacatgca acaacatcta tagcatatgg attttgtgat gctaataaaa ctcctatttc
     5941 tcgagtagta ccagctgaag gtccatcacc ttccatacct actataccat ccataattgt
     6001 caatgttggt tttatataat cacaaatatc taaaagtaca ttttcgcaaa acaactcttc
     6061 tgtaggaaat ctataatgta tctctgcttt tttcaatcca gctatgcatc catataagtt
     6121 ttttgtacca ccagtaaaag ttgccattat atgagtcttt aatttacaaa gatttattat
     6181 ataatctgaa tctactattg gtttaataat atccatatat tttagaacat gtgctttttc
     6241 actatgtatc ttaacatctg aaacatcata atttaactta atgcccagtt catttgctac
     6301 ttcttcaatt ccagatgcct tataaatgct cttaagagaa ctttgattat atggaccacc
     6361 agggctatct gctattgtaa tatcacaatt taactctaaa agtttttcac atacaacttt
     6421 tacaaccata ggatgtgtag ttgtagcttc ttcaggcttt tttttcatta ataaatttgg
     6481 ttttataagt actttgcttc cttttttaac aaacttatct ataccaccaa tatcctctaa
     6541 atttttttca atagataact ttacttcttc ataattgtaa gttttacaac tcctaacaga
     6601 taccgacttc atattatctc catccttcta tcacaacagt ttatttatat aatttaaaaa
     6661 aggagaaacc aaagtttctc cttttaatct atgttttaat tgttttaatt attattttcc
     6721 aaaatgtctg tttaataatc ctaagaatgc tttaccatgt ctagcttcgt ctttacacat
     6781 ttcatgtact gtatcatgta tagcatctaa tcctaattct ttagct
//
LOCUS       DAELJO010000190         6811 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAELJO010000190 DAELJO010000000
VERSION     DAELJO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.192"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            59..2707
                     /gene="polA"
                     /locus_tag="KPB88_003238"
     CDS             59..2707
                     /gene="polA"
                     /locus_tag="KPB88_003238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG0055477.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2716..3318
                     /locus_tag="KPB88_003239"
     CDS             2716..3318
                     /locus_tag="KPB88_003239"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG0055478.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3311..3865
                     /locus_tag="KPB88_003240"
     CDS             3311..3865
                     /locus_tag="KPB88_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0055479.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3865..5580
                     /locus_tag="KPB88_003241"
     CDS             3865..5580
                     /locus_tag="KPB88_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055480.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5653..5865
                     /locus_tag="KPB88_003242"
     CDS             5653..5865
                     /locus_tag="KPB88_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG0055481.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6066..6665
                     /locus_tag="KPB88_003243"
     CDS             6066..6665
                     /locus_tag="KPB88_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055482.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
BASE COUNT         2935 a          639 c         1227 g         2010 t
ORIGIN      
        1 tatattggta tataatatat atgttacaaa aatagataat ttttaggagt gatataattt
       61 ggaaaaaaaa ttaattataa tagatggaaa ttcaataata aatagagctt tttatgctct
      121 tccagaaatg aataataaag aagggttaaa gaccaatgct atatatgggt ttactacaat
      181 gctatttaaa atgatagata tatataaacc aacacatata agtgtagcat ttgacagaaa
      241 agcacctaca tttagacatt tagaatataa agagtataag gctggaagaa agggaatgcc
      301 agatgaactg gcagaacaat tacagccatt aaaagacctt ttagataaat ttagaataaa
      361 tagattggaa atagatggct atgaggcaga tgatattata ggtactgttt ctaaaaaagc
      421 agaagaaaat ggatataagg tatacatagt aactggagat aaagatgcta ttcaacttgc
      481 ttcagataat acaactaccc ttataacgaa aaaaggggtt ggagaagtag aagaatacaa
      541 ctttaattct gtaattgaaa aatatgagat gactccaaca caatttatcg atttaaaggg
      601 tcttatggga gacaagtctg ataatatacc tggagtacct ggaataggtg aaaaaacagg
      661 tataaagctt ataaaagagt tttctagtat agagaatcta atagagaata cagataagct
      721 taaaggtagt gtaaaaaaga aaatagagga aaacaaagaa atagctattc aaagtaaaag
      781 acttgcaaca attataagag atgtgccaat agaagtggat ttagatagta tgatttttgg
      841 cgattatgat aaagatgaac ttttagataa atttagatac tttggattta caagtctctt
      901 atcaagatta gttgatttgg tcgattcaga tgacacagaa cacgtagaag aaaaaataga
      961 aatattaaaa ttaaaagata tagaaaaatt tatagatgaa gtaaacaaaa aaggacaagt
     1021 tattttaaaa actgttagag gacaaggtaa tatacttgaa aaaaatataa tatatatttt
     1081 tataagtgta gatggagaaa aaatatatta tgtagattca aatgaagtgt caaagttaga
     1141 tagtatattt tcaaataatg aaattaaaaa gtatggatat aatttaaaag atgattatat
     1201 atctttaaaa ccatataata taaacttaga aaatatgtat tttgatataa caatagctga
     1261 gtacttaata gattcaactt catctacctc atatgattgt agtgcaatag ctatgaagta
     1321 tttatcaaaa aaggttaaat caaaagaaga attattagga aagggtgtta aatctaaaaa
     1381 ctatgaggat ttagaatttg aggatttagc agagtatatg ggtcaaataa tatgcacagt
     1441 taaagaaaca gttcctatga tggaaagaag tctaatggat atgagtatgg atggtctttt
     1501 ttatcatgta gagatgcctt tagtagaagt cttaggtcat atggagtatg aaggtataaa
     1561 agtagacaaa agtatattgg atgaattgag tgtagagttt aaagaaataa tagctacatt
     1621 agaaaaggaa atttatgaat tatcaggaga gccattcaat ataaattcac caaaacaatt
     1681 gggtgttata ttatttgaaa aattagagct tccaataatt aaaaaaacta aaactggata
     1741 ttcaactaat gcagatgttt tagaaaaatt aagagataag catccaataa tagataagat
     1801 aactgagtat agacaaatag taaagttaaa ttctacttac gttgaaggac ttttaaatat
     1861 aataaatcca gaaagtaata gaatacattc atcatttaat caaactataa cgactacagg
     1921 aagaatttca tctacagaac caaatcttca aaatatacct ataaaaatgg aaatgggtag
     1981 aaaaataaga aaagtgttta ttgcagatga taactgtaag ttggttgatg ccgattactc
     2041 acaagtagag ctaagagttc ttgcacatat gagtcaggat gaaaatatga tagaagcatt
     2101 taaacatcat gaagatattc ataccaaaac agcttcacaa gtttttaatg ttcctatgga
     2161 agatgttact agtgaattga gaggtgcagc taaagctgta aactttggta ttatatatgg
     2221 taaaagtgac tttggtctag ctgatgattt aaatatacct gttccaaagg cgaaagaata
     2281 tatcgaaagt tattttgcta aataccataa aataaaagag tttatggaca caacagtaga
     2341 aaaggctagt gaggatggat atgctgtaac aattttaaac agaagaagat atataccaga
     2401 aataaagtca agcaatttta tggaaagaaa tagaggaaag agatttgcaa tgaatgcacc
     2461 aatacaagga agtgcagcag atataataaa aattgctatg ataaatgttc ataaaaaatt
     2521 ggaagagaac aatttgaaat caaaacttat actacaagtt cacgacgagt tgatagttga
     2581 agcaattgat gatgaaatag atatagttaa gaaaatagta aaagaagaaa tggaaaatgc
     2641 tgtaaatatg gatgttcact tagatgtaga tttaaatgta ggtagttctt ggtatgagac
     2701 aaagtaggtg attttatgtt gatattagga cttacaggag gaataggctg tggaaaaagc
     2761 agcctatcaa atatatttag aaatcttaac attcctatag tggatgctga tattatatcc
     2821 aggaagatat ttgaggataa gttgttattg gaaaaagtat ttgtccattt tgggcaaagt
     2881 ataaaaaatg atgatggaac tctaaataga aaagctcttg ggaaaatagt ttttagtgat
     2941 gaagaaaaac ttaaggaatt aaataacttg actcatccac gaataagaga aaaaataata
     3001 agtgaaatag aaaaattgag aaaaaaaggc gaaaatatag tagtgttaga tgcggcaata
     3061 ttggtagaaa gtggtttttt agatatggtt gacaagctat tagtagttac ttgtaaacaa
     3121 gaagtacaaa ttagtagaat acaaaagaga gataattgca gtgagcagga agcacttagt
     3181 aggataaatt cacagatgag tcaggaagaa aaatccaaat atggtgatta tataatagat
     3241 aattcgggaa caataactga attagaaagt aaagcacata aatttattga gtacatgaag
     3301 gagaattggc gtgaatagta agaaagtatt gattctatct atttttataa tcttatttgg
     3361 ggcactatta atggaaagca aagtaataca taaattttta tatcctaaaa aatattcaga
     3421 gtatgtagaa aagtattcga aagaatttaa tttagatgaa aatatagttt acagtgttat
     3481 taaagccgaa agtaagttta atagttctgc tgtttcaaaa aaggaagcaa aaggattaat
     3541 gcaaatatta gacataacta gagattgggg agcagaggaa ctaaatttaa aaaatgtgga
     3601 tattttcgac ccagagacta atataagact tggctgttgg tatttaagta agttatacaa
     3661 agaatttggt aaattagatt tagtgatagc tgcatataat ggtggttcag gtaatgtgaa
     3721 aaaatggtta gaaaataatg aatatagtaa agatggcgaa aatctacatg atataccttt
     3781 taagcaaact tcaaaatatg tagaaaaagt aaaaaataat tacgaacatt ataataagat
     3841 atatggcaag aaaggaaaaa actaatgaag agaataaaag tcttaacagt cgttttagct
     3901 ataactctta tggtagctgg ttgtagtaat actaaaacaa aacaaagtag tagtgattca
     3961 agtttacaaa gtcaaagttc tatagatgct aagtatataa acttaacaat ggtaactcca
     4021 aaaactataa atcctataac taatactgat aagtctgttg gttatataat gaatttagtt
     4081 tatgatagtt tatttactat agatgagaac tacaatgtaa ttcctcaatt agttaaggag
     4141 tataatatag ctcaggatgg aatgagtata gatataaagc taaaagatgc aaagtggcat
     4201 gatggaaaaa atgttacttc aaatgatgta aactatacta ttggtttaat tcaaaaaagt
     4261 gcagatagtc catataatga atttactaaa aatatagctt ctgtgaatat taaatcagat
     4321 aaagatttta caataaaatt taaagctaga tatgcttttt caattgatag tttaattttt
     4381 ccaattgtat ctcaaaatca tttagattca aaagatgtaa atgacaagaa taataatatg
     4441 ataggaaatg ggaaatataa aatagaatca tatacagaaa gagaaggaat ggttttatct
     4501 gtaaacaaag attactatga agaagttcca aaaaccatga agaacataaa agtaggtatg
     4561 gtgcctaatg aagatgcaag gacatctatg gttatggctc ttgatagtga tataaccaat
     4621 gttacattaa atgatttaag taagtttcaa gaaaaagagt ttaatataac aaaatatcaa
     4681 ggaagagatt atgagtgtgt attatttaat tataataatc cttttttcaa agatgttaat
     4741 tttagaaaag caattgcaca ctctatagat aaagatagaa ttattagtga aggttatatg
     4801 gatgatgcta ctccagttaa ttttccactt aattcaaaat caaaatacta taatagtgaa
     4861 atgaaggatt tagagtttaa taaagataag gcgatagagt gtcttgctaa ggtagaatat
     4921 gcaaatgtaa actcagtaaa tcaaaatgac aataaagtaa aaaatcagaa aaaatcagct
     4981 aaaaagaact caaagaagct tacgccagaa gaagaagcaa aagctaaaaa agcagaagaa
     5041 gatagaatca aaaaagaagc tgaagagaaa aaaaatagag aaaaagagga agtaaagaaa
     5101 agtctttctg aaatgaactt aaagataata gtaaacagag ataatagtga gagaataaaa
     5161 actgcaaaca taataaatga aaacttaaaa gctataggaa taaatagtac agtaaatcag
     5221 ctgtcagata aggatatgga aaatgctctt aattctaaaa attatgattt agcattagtt
     5281 ggatggaaac tatcaattat accagatgca agtagtatta tagcgagtag tggatatact
     5341 gatgataagt taaatggata tatgtctgca cttacaagtt ctacgtctga aattgaaaca
     5401 aaaaaagcat acaaagatgt acagacatat ataaaagata atgcaacatt tataagttta
     5461 gctgtgagaa acaactatat tgtaagtaat agtagattaa aaggtaaaat tactccaaat
     5521 gattttgatg tatatgaggg aatatctaat ttagatataa aatcgaatga atcaaattaa
     5581 atgattaatt tctatttaaa aagtataaac tatatgatag aataccctta tagggtataa
     5641 ggaggtaatt aaatgaagaa gaaactatta gtagaaggta tgagttgtgg tcattgtgta
     5701 aaccatctta aaactgcatt aacagaagat atagatggta ttgaagtttt agatgtagat
     5761 ttagaaaata agtgtgcatc tgtagatatg aaagatgatg tatctattga aaaattaaaa
     5821 gaagtgatag cagaattagg atttgaacta aagggaattg aatagaaatt gataaactaa
     5881 aaaaagacct ctattggggg agtaatagag gtctaaaaat gggggagatt gagtatgttt
     5941 gattttacat tactattatc tcccctttta aattaatata tacataaaaa gtaaaaaaat
     6001 tttgtaatga ctcacatttc ttgagctttt tgaatatact ataaatagct aagggaggga
     6061 gtatcatgga atctagggga tataaagagg atttaaaaaa aatgatactt gatatggccc
     6121 acaaagaact tgagagttat gtatcacaaa aaggagcaaa aaaatacttt gaaggtaact
     6181 tggataagat tattaataat aatataaaaa aattcaatca aagtattggt cttaatagag
     6241 tatatttaaa aatgttaaaa aagtgtgcat atcaagaaag tgtaacttca atatcaagga
     6301 ctataaagaa agaagcatta tttaaatcaa aaaaagagct atttgctttt tcagagtact
     6361 taaaacttga tattaacaag cgactttcat ataatcaaat tttaagaaaa atatcaagat
     6421 atatatacta caatagaagt tcatatgctc aaaaatacgt tgttttcaaa agaggagatg
     6481 aagaatatct tttagaacca gagaaaataa aagatgaatt aatatcaagc tataggtcaa
     6541 agactagaga agatatgagg tcaatagcaa aacttttgga tatagaagta gaagatgatt
     6601 ataatgctga agatattaga aaaaaagtaa taaattatat tatcaaagaa aaacttaaaa
     6661 gttagagttt taaagaataa gttctgccaa tttgcatgac taagtataac ttgggtataa
     6721 attttttatg catttgaaag agcttattct ttttttgaca tattctttta aataatttac
     6781 catgatttgg taattggtca tcatcattca a
//
LOCUS       DAELJO010000191         6802 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELJO010000191 DAELJO010000000
VERSION     DAELJO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.193"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..706
                     /locus_tag="KPB88_003244"
     CDS             <1..706
                     /locus_tag="KPB88_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG0055483.1"
                     /translation="NMVKDFQGRIYNIDTPVIFVCSIFEGLEKFDIQLALRNKLLIDG
                     YKVLQIGTRQVCELFDFYSFPEFMYSNNIEEQDKVLMLNNTLKKLELEKKPDLIIIGI
                     PGGILPFSSKAFGDFGITMYKVLQAVTPDCTILSIPYGKYSDEEISYFGKITKSKFNI
                     EVDYYNIAAKSILSHPTETMGKPFYLTLGKSLVEKKIATLQNKNVFCLSNKSEIDRLI
                     ELMIKEFIKYGTVASI"
     gene            740..1030
                     /locus_tag="KPB88_003245"
     CDS             740..1030
                     /locus_tag="KPB88_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG0055484.1"
                     /translation="MVNTEVMNKLDIYTVLVNIFEKRSKINFDENCYLKNENLFESKI
                     MMPARELVLTLYDIEDYFSISIPEDVILSGRFNSFNNIYDIVKKLENSINFN"
     gene            1072..1290
                     /locus_tag="KPB88_003246"
     CDS             1072..1290
                     /locus_tag="KPB88_003246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055485.1"
                     /translation="MSNFGKRNLYDSANGEIDPKGGCNCGALCANLCSCSCSGGTWSF
                     YHTNQVKGSKINNGQQFRSPGEIPGQKK"
     gene            complement(1494..3161)
                     /locus_tag="KPB88_003247"
     CDS             complement(1494..3161)
                     /locus_tag="KPB88_003247"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0055486.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMNDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNSY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(3358..4080)
                     /locus_tag="KPB88_003248"
     CDS             complement(3358..4080)
                     /locus_tag="KPB88_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055487.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            4471..5898
                     /locus_tag="KPB88_003249"
     CDS             4471..5898
                     /locus_tag="KPB88_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0055488.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            5891..6670
                     /locus_tag="KPB88_003250"
     CDS             5891..6670
                     /locus_tag="KPB88_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG0055489.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
BASE COUNT         2471 a          866 c         1047 g         2418 t
ORIGIN      
        1 aaatatggta aaagattttc aaggaagaat atacaatatt gatacacctg ttatttttgt
       61 atgtagcata tttgaaggct tagaaaaatt tgatattcaa ttagcattaa gaaataagtt
      121 actaattgat ggatataagg ttcttcaaat tggaacacgt caagtttgcg aattatttga
      181 tttctactca ttccctgagt ttatgtactc aaataatata gaagaacaag acaaggtact
      241 aatgcttaat aatactctaa aaaaattaga attagaaaaa aaacctgacc ttataattat
      301 cggaattcca ggtggtatac ttccattttc ttcgaaagca tttggtgatt ttggaattac
      361 aatgtacaaa gtacttcaag cagtaactcc agactgtaca atactatcta ttccatatgg
      421 taaatatagt gatgaagaaa tttcttattt tggtaaaatt acaaaaagta agtttaatat
      481 agaggtagat tattacaata ttgctgcgaa atcaatatta tctcacccaa cagagactat
      541 gggaaaacca ttttatttaa cattaggaaa atcattagtc gagaaaaaaa tagccacatt
      601 acagaataaa aatgtatttt gtttatctaa caaatctgaa atagacagat taatagaatt
      661 aatgatcaaa gaatttataa aatatggtac tgtagcatct atataaaata ttttacataa
      721 aggatggtga aatactgata tggtaaacac agaagttatg aataaactag atatctatac
      781 agttttagta aatatatttg agaaaagatc aaaaataaat tttgatgaaa attgttattt
      841 aaaaaatgaa aatttatttg aatctaagat aatgatgcct gctcgtgaac tagtattaac
      901 attgtatgat atagaagact atttttctat atctattcca gaggatgtaa ttttatcagg
      961 aagatttaat tcttttaaca atatttatga tattgttaaa aaacttgaga attctattaa
     1021 ttttaattaa ttaaaataca ataatataac aaatgagagg ggaaaaatga tatgtctaat
     1081 tttggaaaaa gaaatttata tgattctgca aatggagaaa tagatcctaa aggaggatgt
     1141 aattgtggtg ctctgtgtgc aaatctatgc tcttgtagtt gttctggggg aacatggtca
     1201 ttctatcaca caaatcaagt gaaaggttct aaaataaata atggtcaaca atttagatca
     1261 ccaggagaaa tacctggaca aaaaaaataa aaagagttaa atataattag ttttagcttt
     1321 atcaattaac ataattaact ttattaaaat taatagaatc taattacaat tgtttattat
     1381 atctataaaa gcaactttaa aataccttgt tttatatatg ttaattaaaa atggtaggag
     1441 ctgtcgtgct agaaaatagt gctacagctc ctaccatttt tacataaata actttattta
     1501 taatatatca ttatactttc gtaaggtttt atttccattt tattttcaat tatatctcta
     1561 gtgtaattgg tttcaagtat tgtgtagttt aatttattca aataagtttc aggtaattta
     1621 tatactacat tatcatgagt aaaattactt ataacaagaa gattttggtt tttatactct
     1681 ctttcataaa caaatagatt tttatgattt tcatctaaca atctataagt accttctgta
     1741 aaaatatcat attgtttacg atatttgatt aatctttgat aagtataaaa gactgaacga
     1801 gaatcattaa gctcattttc tacactaata ttgtcattat taacttttaa ccacggttta
     1861 tgattagaaa accccttata aatagtatta tcccattgca taggagtacg ggcattatct
     1921 ctggattttt gttgaagtat aagcaatgcc tccttttcat caataccaga tttaatcata
     1981 tgatagtatg aattcttact ttcagcatca tcatattcat ctattttagt aaagtaggca
     2041 tttgtcatac ctatttcttc accttgatat atgtatgtag taccttgtag accaaatagt
     2101 gttatggcta acatcttagc actttcttga cgaaaaaatc tatcatctcc aaatcgagat
     2161 aaagctctag gttggtcgtg attagagtaa aatagagcat tccatccctt tccttcatac
     2221 attttatatt gaaagtcaga ttgtgctttt tttaatttat ccagagagaa catttcatta
     2281 gtccattttg cgcctccttt ataatctgtt ttcatatgat ggaagctaaa aaccatagat
     2341 agttcttctc gattttctct agtatagttg atacactctt caggtgtagt agatgacatt
     2401 tcacctacag taactatatt tttaggtttg aaaacttttt gattcatatt atgtaaatat
     2461 tcgtgtatac gaggaccatc agcgtagtga gttcttccat ctccttttgg tcctgttata
     2521 gggtcatcag gtaatccagg tgttttggag agtaggttga ctacatctaa acgaaaacca
     2581 tcaatacctt tatcagccca aaattctagt attttgtaac attcttcacg aaccttttca
     2641 ttttcccaat ttaaatcagc ttgagtttct tcaaataaat gtaaataata ttggttagtg
     2701 gtttcatcat atttccaagc agttccacta aatctggata tccagttatt aggaacagaa
     2761 ccatcaggtt tagcatcttt ccagaaataa taatcacgat atggattgtc tttgcttttt
     2821 ttagattctt taaaccattt atgttcagta gatgtatggt ttaggaccat atccatcatt
     2881 atttttatgt ctcttttatg agcttctttt aagagttttt caaagtcatt cattgtccca
     2941 tattttgggt ctatattata ataatcttct atatcatatc cattatctct ttgaggagaa
     3001 acgtacatag gagtcaacca taataaatca acacctaaag aatacagata atctaatttt
     3061 tctataatac catttatatc acctatacca tcattgttgc tatctttgaa actttttgga
     3121 tatatttgat agacagttgc ttttttccac caatttttca tacttgatac ctcctgtaat
     3181 ttataagtta attataatat gtttaaacaa attgtcaagt attaaactga tattaaagct
     3241 aaaatataca ataaataatt atttgtataa acatataatt gtatatagaa tagtgaggtt
     3301 agtatgggat aaagtgtcta aacgtaaaaa aattatgttt aattaaacat aattttttta
     3361 atatctttga gtaacatcca tgaatacaaa tgtttcaggt gtatgctttg attcagtata
     3421 ttcaaaaagc tttccatcat cagtatatac actattttta ataacaccaa cacaattata
     3481 atcatttaga ggtagcgttt ttaactcaag ctctgtagct ttttcaattt taaatatctt
     3541 tctggatgaa acaatttttg tacctagaca ctcttctata tatttataaa tagagccttg
     3601 tgctatagaa acatcaaggt ctttaactat actacataga aaatagttaa tgtcgagaat
     3661 tttaggtatg ttatcaatat atcttagtct gtgaaggtaa tatacttcac taccaacttt
     3721 aaaacctgtc tttttagata acttattatc aattaagatt ttgctgaagt ggagaacact
     3781 agtagcagta ttaacctttt tatcttctac aatcgattct acacctttga aattgtgcat
     3841 atttagaaaa aaatcattag aacaactatt ttctaacact acaacaccct tgccttttat
     3901 actttgaaca tatccttctg tgtttaattg agaaattgct ctacgtacag tgtttctgct
     3961 acatgaaaac ctttcaataa gttgaagttc agtaggcagc atcatattac ttgtatattt
     4021 accatcaagt atttcctctt tcaatacttc atatatctct gaaaatttat ttttagccat
     4081 atacccctat ccctccttaa aggtctttag taatcttatt gaactgatat gaacaaatta
     4141 tatcaaatgt gtttagacat gtatagtgaa aatagaatca tatcaaccat aaaaaggaca
     4201 aaatagaatt attttgtggg ttgtttcaaa tacatggtta aacatatttt taaaataaaa
     4261 aaaatgaatt aaaaaaatag gctagatata gcaatacact taaaaggatt tatatgttaa
     4321 accaaatgat aaaaataaaa tgaataaaaa atattgacaa tttgtttaaa caaatataat
     4381 atttaaaata taaaatgtga ataaatcgtt ttcataaaaa attaaaccga aatagaaagg
     4441 ggattgtata tgaagaggtt cttaaacaaa atgatgcaac aactgtcact tttaggaaag
     4501 actatgctgg tgccaatagc tgtaatgcca gcagcaggta tattaggatt attatttggt
     4561 ccaaatatgc tcaatgtgcc agctatatct aatatatcta atatagtatt ttcaaatgtg
     4621 gactacttat ttttattagg agcagctagt gcctactcaa agtctaaaga caagacaagt
     4681 gtaatggttg cagccgtagt tgcatatatg atatttaagt caaatttaga gttattgaat
     4741 gatacattaa atgcaggtgt atttggtggg ataattgttg gagcttgtat agcatggact
     4801 tacaataaaa catataaagt taaagttcca gcatttttaa gcttctttgc aggagaaaaa
     4861 tgtgtaatta cactatcacc agtggttgct ttaattttat catatatatt tagtttatta
     4921 tggatatatc cacaagctgc aatgaatcaa tttggttttt ggttgggagg agctggtgca
     4981 ataggaatat tcttgttttt attctgtaac cgtgcactaa taccaactgg gttacatcaa
     5041 gttttaaatg cttacatatt atttgaaatg ggtgaatata caacatcaac aggagaagtt
     5101 ataagaggag aaattcctag atttatggca ggagacccaa atgcaggatt cttctggaca
     5161 ggattttaca taattatgat gttttcaata cctgctattt cttatgctat atataaaaca
     5221 gcacaagata aaaagaagca agaagtaaaa ggtgcaatga ccgctggagc attgacttct
     5281 tttatggcaa cagtaactga accaatagaa ttttcgtttt tatttgcatc tccaaagtta
     5341 tatataattc attcttttta ttctggtcta ggtggagttg tactatattt actgggtgct
     5401 cgcttgggtt catttaatgg agcaagtata attgactatg tcttaagttt tagttatgga
     5461 gataaagcgt ggttagttat acctgtagga gttgtattct ttgtattata ttatgtaaca
     5521 tttaaatata taataataaa agataatgtg caaacaccag gacgtgaagt tgagatagag
     5581 ataggtgata aagttagtga aaaagaaaag aatttaaaac tttctcatgg taatttttca
     5641 tatatggcta aacagataat taaaaattgt ggtggttatg aaaatatagt gactcttaat
     5701 aattgtatga cgagattacg tttagaagtg aaggatgcca ctattttgaa tgatgataat
     5761 attaaaaaaa caggagccaa aggggtaatt aaattgagta atacatcagt tcaaatcatc
     5821 ataggaacgg atgttgttaa agtaaaggat gagatggaaa tgcaacttga tgagttgaga
     5881 aagcaggcag atgcataaaa tgaagaaatt agtagttagg gcagatgata taggatattc
     5941 agaaggtgtt acactaggga taatatcttc ttataagaac ggtattgtta caagtgctgg
     6001 aatcatggtg aatatgcctt tttcaagaca agctatagaa atggtaaaaa atgatgatgt
     6061 atgttttggt ttacatgtta atttagtaat aggagaacct tgttgcaaag aacatattaa
     6121 gataaagaat ttgttagatg aaggtggaaa atttgtttct agcaaaataa gaagaacgca
     6181 actactgaca ggtaaagagg tttttaatta tgaagaagta tatgaagaag tttgtgctca
     6241 aattgaaact tacaagttgt atatgagtaa attgcctgag tatattgata tacatggtat
     6301 agaagaagat gtatcaatat cagcaatgtg tgatgctgca aaagaatacg gtataccaag
     6361 ttgtccctat tatttggatt catgtattag catgccaagt aaatatcctc agaatgattt
     6421 ttacaagtta aataaatctt ttgttaaatt atttgaagaa aaatatataa atttagataa
     6481 agatataaca ttgatggtta ctcatccagg atttatagat tatagtctta taaaatcatc
     6541 ttctcttatt caagagagag tatatgaaca tgcaatgcta tgtgataagg aagttaaaat
     6601 gtggttaaaa agaaataata ttgaattggt tgattttaaa gatattttca agtctaataa
     6661 ttatatataa agaatagatt acctaaagtt ttaattaaaa ataagtattt gaaaaaaatg
     6721 tgtattttct aaaaaaatat tgatagagat gtatcaaaat aaaaacttga atcatctcta
     6781 tcaatatagg aagtacacat tt
//
LOCUS       DAELJO010000192         6778 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELJO010000192 DAELJO010000000
VERSION     DAELJO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.194"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1179
                     /locus_tag="KPB88_003251"
     CDS             <1..1179
                     /locus_tag="KPB88_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0055490.1"
                     /translation="ILAMGGYIFYFKNFDKEIKEIDEYPMISILVPAHNEAKVIGRTV
                     ESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYNFTIINTDSLTGGKGKSN
                     ALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMDDELGAVIGKFRTRNKNKNLL
                     TKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIEEIGGWDSKAIAEDTEIS
                     FRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNIYVLMKYIKNIFKQGRNK
                     IVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISIPINFFLIWILSYLLFIIEVS
                     ISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYLKDIIFKREVKWYKTERF
                     "
     gene            1202..3283
                     /locus_tag="KPB88_003252"
     CDS             1202..3283
                     /locus_tag="KPB88_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG0055491.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLRGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            3320..4405
                     /locus_tag="KPB88_003253"
     CDS             3320..4405
                     /locus_tag="KPB88_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0055492.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4459..5583
                     /gene="wecB"
                     /locus_tag="KPB88_003254"
     CDS             4459..5583
                     /gene="wecB"
                     /locus_tag="KPB88_003254"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG0055493.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENINSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5661..6635
                     /locus_tag="KPB88_003255"
     CDS             5661..6635
                     /locus_tag="KPB88_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0055494.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2832 a          643 c         1130 g         2173 t
ORIGIN      
        1 attttagcta tgggtggata tatattttat tttaaaaact ttgataaaga aataaaagaa
       61 atagatgaat atcctatgat atcaatactt gttcctgctc ataatgaagc taaagtgata
      121 ggtagaactg tggagtcttt gctattactc aattatccta agagtaagat ggaacttatt
      181 gttataaatg acaattcttc tgataatagt aaagaaattt tagaaaatat aaaggatagg
      241 tacaacaatt ataatttcac tataataaat acagatagtt taacaggtgg aaaaggaaag
      301 tctaatgcat taaatattgg atatacgatt tcaaaaggag attttatagc tgtttatgat
      361 gcagataaca caccagataa aaatgcactt agatatctag tacaaactat agtaatggat
      421 gatgaattag gtgctgttat tgggaaattt aggactcgaa ataaaaataa aaacctatta
      481 accaaattta taaatataga aacattaagc tttcaatgga tgtcacaagc tggtagatgg
      541 caacttttta atctatgtac aataccagga actaacttta tattgaggag aagtatcata
      601 gaagaaattg gaggatggga tagtaaagca attgcagaag atacagaaat aagttttagg
      661 atatataaat tgggatacaa gataaagctt gtaccacaat ctataacatg ggaacaagag
      721 ccagaaactg ttaaggtgtg gataaagcaa cgtactagat gggcaaaagg gaatatatat
      781 gtattaatga aatatataaa aaatatattt aaacagggaa gaaataagat tgtgtttgat
      841 atagcttact ttttttctgt atattttcta tttttaacat ctgtaattat atccgatatt
      901 ttatttgttt taagtatatc taaactagta gaaatttcta ttccaattaa cttttttcta
      961 atatggatac tatcttatct tttatttatt atagaagtga gtataagcct tactatagaa
     1021 aaaggtgaag caacaattga aaatatattc atagtggcaa taatgtattt tacatactct
     1081 caactatggc tgtttgtagc tataaaagga atgatagagt atctaaaaga tataattttt
     1141 aaaagagagg ttaaatggta taaaacagaa agattttgat tcatgtacag gaggaattaa
     1201 catgaaaaaa tttattatat caattatatc tttagtattg tttttttcaa atatatcact
     1261 tatatacaaa gtaaatgctg atgagactaa agttaaaaat tataagtttg aaagagatat
     1321 aactattgat ggtgttatag gaagtaatag tacttttttt gaagtaaata aaaattggga
     1381 tattgaagaa gttttattgc atcttaattt tagtaagagc caaatattaa atggagatgt
     1441 ttcaagttta actgtattga taaataatgt accaataaaa tctattaaat taaatgctaa
     1501 gacaaattat aaaaatacat tagaagtatt agttccaaaa gattatataa ttcaaggata
     1561 taatgaaata aaaataaaaa catacaaaac aatatctgat aaaatttgcc aagatgattc
     1621 caatactggg aactggatgg ttattcacaa agaatcatat atatcaatta gatataagca
     1681 gaaaaaggtg gaaaactcaa taaatgaata tccgtatcca tatgcagaaa ttgaaaataa
     1741 tcataaatta gatacaacta tagtagtacc agataatatg actagaggag agactacagc
     1801 agtgtttaat ttggcatcag catttggaaa aataacaaaa aatgatgatt taaagttaga
     1861 tgttaaatta tattcagaaa tgaaaaattg gtcagatgat aatattatat atattggtaa
     1921 acctgaaaat acagcagaag aaattttgga tattttaagt ataaaggaac aaaccctatt
     1981 atcttcaaac tgtataatca agcaagtaga ttcaccatac aataagaata agaagatgat
     2041 ggtggtaatt ggtagtaatg aagatgattt aataaaggca tcaaatttat taatagaaaa
     2101 tagactttct aaccaagttt tatcatcatc agtacttgta aacaaagaga ctaatataaa
     2161 aattaatagg gaacaaaaat taaatttagg tcatttaaca ttaaaagact taggatattc
     2221 tgactttttg ttggaaggag catttaatca acaagctttg tttgatgtca aaataccaac
     2281 gggtaaggta ttagatgatg gttcaaagat tatcttaaat ttaagatatt ctgacaattt
     2341 agattttgaa aaatctttag ttacagtgtc tataaatgat gttattgtgg gaagtaaaaa
     2401 attggatagg tcacattcta ataatgataa gttagaatta aaaataccta aagatataga
     2461 taataaaaac tattatcaag ttaaattgac atttaatttg agtataaaaa attcgaattg
     2521 tgttactaga gagagtaata atccttgggc atatgtatca aataattctt atttggcgtt
     2581 atctacgaaa gaaaatgaaa ccttatcttt tgaaaattat ccgtatccat ttgttagaga
     2641 tgatgaattt aatgatttaa ctgtgataat gccagattat tcagggtcac aagctatgac
     2701 ctggatgttt agattaggtg ttactttagg tgcgaatata aattcacata atggaaatat
     2761 aaatgtaatt agaggaaaag aatttagtga caagtataag gatactaata ttgtagtttt
     2821 tggagtgcca cataataatt ctgttataaa gatgttaaat aataatttaa atataaagtt
     2881 cgataaaaat tactccaatt ttatatcaaa tgataagata agctttattg atgattatgg
     2941 aaaaaatatt tctactattc aattaataaa atcaccatat aataatcaaa aaaatatcat
     3001 ggtaattagt tcaatgaatg aaaaaaactt atatttagga atggattatt tgttgaataa
     3061 aagtaaagtt aatgatttaa gaggagatac actgataatt gatgaatacg gagaggtaga
     3121 agatttagct tataatttaa aacctaagaa agaagtaaaa gattcaagct ggaatatgag
     3181 tataaataag acaactaaag tatttttaat gatttctttt attactataa ttgtagtcat
     3241 gatattatca atgctatata taaaaaaata taaaagaaga taatatgata aatttattat
     3301 tttaaaagag gtatataatt tgaggaaata tatgaataaa tttaaattaa aaagaagtat
     3361 tttaatctca ttaataatct taatttttat atttatagtt tactttgtaa gtattgagtt
     3421 aaaagatagg agtctagtaa ataatcaata taaaggtaag attaaatcgg ccaatttatc
     3481 tgtagattat gaaataaatc aagttatgaa ggatatagat aaattgggat tgaatacagt
     3541 aaatgtacct ataattataa atgtagagtc cattaattct gatattatgt caatagataa
     3601 taatagtaaa gaaaaggcaa ttaagttaat aaaaaaatta aataaaaaag gtatttcaac
     3661 aatattagag gcatatccat ggattgaaaa tggaaaattg tatgagactg actggaaccc
     3721 tataaataaa aaaagatttt tttatacttg gcaaaatgcc atattaaatg aacttataat
     3781 tgatgtagca aatccactgg atgtaaatgt tttaaatatt ggttctaatt ttgtacattt
     3841 ggaagaatat caacaaaatt ggggtgaaat aatagatttt gtacaatcca aatttgacgg
     3901 tcttgtaacg tacagaacta attggtggta tacaaaaaaa gatgatttaa gtagtaagtt
     3961 gttttatgaa caaaagctga acaataattt ttttgataag ttagatttta tatctatagc
     4021 agcatatttt gagttaagta ataaacctgt taatacagtg gatgaattaa tttctgcctt
     4081 acactcatct actgtaaaca atagaggaca aaatataaaa caagaaatat ataaccttta
     4141 taaagctcat agaaaaccta tattttttgg agaattaggt ttttcaaata gagaaagtgc
     4201 aagttctcag ccgtggaatc atactccttc aaaagctgta aatggcgaag aacaagcaag
     4261 gtgttttgag gcatataaga aagtttttga aaatgaagac tggataaatg gtttttctgt
     4321 gttttgtgta ggtaaaatag atgacgaaaa aaatttttac ccatcaaaag agagtataaa
     4381 agttattaag tcttggtatg aataattttg aaagtgtaga atttatatag attaaataga
     4441 tgattaggag agatataaat gaatgacatc aaggttatga ctgtatttgg aactagacct
     4501 gaagctatca aagtagcacc tttaataaag gaacttgaaa aaagagagaa tataaatagt
     4561 attgtttgcg ttacagctca acatagagaa atgcttgacc aagttataga aacctttaat
     4621 ataaatgtag actatgattt ggatataatg gaaaaaggac aaagcctgaa tgatataaca
     4681 tgtaagattt taaataaact tcccttgatt ttaaacaagg aaaatccaaa tataatattg
     4741 gtacatggag atacaaccac aactctagct acaagtttga ctgcatttta taataaaact
     4801 cttgttggtc atatagaagc tgggcttaga acctatgata aatattctcc ttttccagag
     4861 gagttaaata gacaacttac aggtattatt gctgatatgc actttgctcc tactaatttg
     4921 gctagaaaaa acttgatttc tgaaggaaaa cctaataata atatatttgt aactggtaat
     4981 actgctattg atgctttaaa gatgacaata aaagaaaact ataatcatcc tataattgat
     5041 gagattggta acgataggat gattttgtta acctctcaca gaagagaaaa tttgggtaaa
     5101 cctatgaaaa atatattcag agctataaag cgaatagtag atgactttga agatgtacag
     5161 atagtatatc ccatacattt aaatcctaaa atacgaacta tagctgatga gatatttgga
     5221 aaatttcctg aaaaaattca tattatagag cctttagatg tagctgattt tcataacttt
     5281 ttaaataaat cttatatgat aatgactgat agtggaggaa tacaggaaga agctccatca
     5341 ttaggtaagc cagttcttgt gttgcgtgat aaaacagaaa gaacagaagg aattgaagca
     5401 aagacactta aattagtagg tactaatgaa gatagaatat ataattcagt gagcgattta
     5461 ttaataaata aagataacta tgttcagatg agtaaagcat ctaatccata tggagatgga
     5521 aatgcaagca aatatatagt ggatataata attaaaaaat ttaattgcaa atatttaaat
     5581 tagataaggc ctaaaacatt aactaattga tataagattt atgataatat taaggaatac
     5641 aatttttaag ggggagtaat atggctagaa aattaataaa aaatttgggt aaaagtaaaa
     5701 gtgtaaagag agtgaaactt ttatttaaga agatttttat tactgtattc atagtagcaa
     5761 gtatagttgc tatttttaat ataacgaaat attttgagga attatacaaa gtaagggatt
     5821 taaaaagtac taaaattgaa tactatatgg atgtggctga tgaagcagga gatggaaagg
     5881 tccaattaag ttggaaggcc ttacttgcta tagacatggt aatacatgat gaagatttaa
     5941 gtaatataaa aaagaaagac acattggata taggggaaaa gtttatagta gaagataaaa
     6001 atgataaagg cgaaaaggtg tataaggtaa aaaagtttaa taaggtatta agcgaattga
     6061 aatttgactc ttctcaaaaa agtagagcaa gaaaatacat gaaagattta gaatacacat
     6121 accttggaaa taaacaatta gatagtagtg atgaaaaaat taaatttata aagaagttag
     6181 aagactcagc tataagagaa tatattgatt atggaatatt gccctctata acaattggac
     6241 aagctatatt agaatctggt tggggaaatt ctaaacttac aaaacagagt aataatttat
     6301 ttggtataaa agcagataaa gcatggaaag gaaaaagtgt agaaatttca acttcagagc
     6361 attataatga aaaaattgta gctagtttta ggtcgtataa ttcattacaa gattctgtca
     6421 aagaccacag tttattttta attaataata aaagatatag aaaacatgga ttgtttgagg
     6481 caaaagatta tattagtcaa gctcaatcat tagaaaatgc aggatatagt acagcagaag
     6541 ataaaaaagg aaatcgtata tatgcagagc tattaataga tgttattagg agttataatt
     6601 tgcaattgat agataataaa gttgagacaa aataattgtt aactttatat gaggagataa
     6661 caagataaga ttcttttcta caaaaagaat agaaaaattg taaaaaaaac taaaaaaatg
     6721 tattgacttg ttatttaaaa ggtgatatta ttattactgt actcgaaaga gaacaaaa
//
LOCUS       DAELJO010000193         6694 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELJO010000193 DAELJO010000000
VERSION     DAELJO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.195"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(468..2855)
                     /gene="cadA"
                     /locus_tag="KPB88_003256"
     CDS             complement(468..2855)
                     /gene="cadA"
                     /locus_tag="KPB88_003256"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG0055495.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTINISNNFKEDDVINKIKEIINSTEPGLD
                     IQVKSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2878..3243)
                     /locus_tag="KPB88_003257"
     CDS             complement(2878..3243)
                     /locus_tag="KPB88_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055496.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3442..4245
                     /locus_tag="KPB88_003258"
     CDS             3442..4245
                     /locus_tag="KPB88_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055497.1"
                     /translation="MSNNKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGMPNNMSNNMNSNM
                     GMPNNMSGNMNNMYGDTGMPNNMNSNMGYNMNNTMGIPNFSCNQNMPPNVLMMIPGVI
                     CHNTMQGMPVVMPSTMPPNIYTTPYGSSNMSTQGIPQTTNIEEFDEEEM"
     gene            complement(4683..5771)
                     /locus_tag="KPB88_003259"
     CDS             complement(4683..5771)
                     /locus_tag="KPB88_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055498.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEIFDEISKSEFVSVYSVNSPEEFAVVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5896..6624
                     /locus_tag="KPB88_003260"
     CDS             5896..6624
                     /locus_tag="KPB88_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0055499.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2351 a         1103 c          720 g         2520 t
ORIGIN      
        1 cttaaggaca agtaatacta tatcacctta aaaataattc gtcaatactt ttttaaatta
       61 ttttttaaaa atatttactt tcttatatta cataatttat tgaatagcag ttatattgtt
      121 ggtttagagt attttaacac acacattttg ttgtaaaaat tataactatt aatataatat
      181 atatacaatc ataagtatta taatttttat gaatctaatt taaacttaat ataaatttat
      241 taataaacct tctatgtcta aattttaaaa ttacaatata aaagctctac actaaataaa
      301 tacattaaat aaacataatg gataatgaat ataaatttat atatgcaaaa gcactccaat
      361 aaaaaggtac caataaagtt aaaagaaact tattgatacc tttcattatt acatacttca
      421 atatataatt ataagttttt tgaatagtat ggatttacat ttatccctta tctcatagct
      481 ctcatagcat ttactactgc tataagtgcc acacctacat cagcaaatat agcttcccac
      541 atagtagcta ctcctccagc acctagtatc ataactataa tttttactcc tagagcaaat
      601 attatgtttt gccacactat cttattagtc ttgtttgcta tttcaattgc tttagatatc
      661 ttgctaggct catctgtcat aagcactaca tctgcagctt caatagcagc atctgaacct
      721 aagcctccca tagctatacc aacgtcagca cgagctaata ctggagcatc atttattcca
      781 tcacctacaa atgctatttt ctctttttca gttcttcctt catataaatc ttctaatcta
      841 tctacctttt cattaggaag taaatttgag taaactgtat ccaattctaa ttcttgagca
      901 atattttttg ctactttctc attatctcca gttaacatta caacttcttt tacccctatt
      961 gcttttaagc ttcttatggc atttttacta tcttctttta cttcatcaga tataactata
     1021 taacctctat atactttatc aacagcaata tagactactg tgcctacttc tttagctgat
     1081 gaataagata tattttcttt tttcataagt ttctcattac ctgctaatat aaaattgcca
     1141 ttatgcttta ctctaattcc ataagctgct atttcctcat aactgtctat tgtatctaaa
     1201 tcaatttttt tattatagta acttaatata gactttgcta ttggatgatt tgaatttacc
     1261 tccgcaatag ctgcatattc taataattct tcttctgata tcccttcagg atttaactta
     1321 gttacattga acacaccttt agtaagtgtt cctgttttat caaatactac tgtatctaca
     1381 cttcttaaag cttctaaata gttacttcct ttaataagga taccattttt agatgcaaac
     1441 ccaattccac taaagaaact aagtggtatt gataaaacta atgcacaagg gcaagaaacg
     1501 actaagaata tcaatcctct atataaccaa tcactaaata cctcacctga gattacaagt
     1561 ggtggcacaa atgctattaa taaagcagct attactacta tcggagtata gtatcttgaa
     1621 aacttagata taaagttttc tgtctttgat tttttaatgc tagaattttc aacaaggtca
     1681 agaatcttag aaactgttga ttctccaaaa ctctttgtaa cttcaatact caataaagca
     1741 tttttattta taacacctga taagatttca tctccttttt caacttctct cagcactgac
     1801 tctcctgtga gtgcagaagt atctaacata gaaactccat ctactactac accatctaat
     1861 ggtacttttt caccaggttt tactactatt atatcaccta catttacctc ttctggtgac
     1921 accaccttaa cttcagaatt aacttttaga tttgcatagt ctggtcttat ttgcataagt
     1981 gaagttatag actttctgga tttaccaact gcaacacctt gtaaatactc tcctacttta
     2041 tagaaaagca taacccctac tgcttctgat aactccccta ttgctaatgc tcctactgtt
     2101 gctagagcca tcaaaaagtt ctcatcaaat actcttcctt ttgatatatt tcttattgct
     2161 cttaaaagca catcaccacc tactattaca taggcaacta taaatactat attgctaaac
     2221 tgactttcat aacctgtagc tgtttgatat ataccaaata tatatacaag tgctccaatt
     2281 ataagtggaa ttaattcttt cttatttgta tcatttacag ctcctgattt ttcagtagtt
     2341 ctccctttta ctttgtcagt agactttact tgtatatcaa gccctggctc tgttgaattt
     2401 attatttctt ttattttatt tataacatca tcctctttaa agttattact tatatttatt
     2461 gtaagttttt tatttacaaa gtttaaatta gaagattcaa cttcttttaa tttagaaacc
     2521 ttgttattaa tctcctctgc acaatgtgca cagtttaatc ctcctaatat aagctctttt
     2581 ttaattgatg ttttactaga tgcattttct ttagaactta tttgtatatc aagacctggc
     2641 tctgttgaat ctattatatc tattatcttt tctatagtag tatcctcatt aaagctttcc
     2701 tttatattta ctgtaagttt tttatttata aaatttaaat tagaagattt aacttcttgt
     2761 agtttagaaa ctttattatt aatctcttct gcacagtgag cacaatttaa tcctcctagt
     2821 ataaattctt ttttaattga attatttgct tccatatata ttcactcctc tcatttatca
     2881 tttataagtc tctctaatat gatttaatcc acaatcaaat atctgactta tatgactatc
     2941 atctaatgag taatacactg tttttccttc tcttctaaac tttactaatc tagcctgctt
     3001 taatactcta agttgatgag atattgcaga gtgagtcata tttaataaac tcgctatatc
     3061 acatacacac atttcgtttg caaataatgc atataatatc tttactctag tagtatcccc
     3121 aaatacttta aataattctg ctaaatcata taacatctct tcatctggca tagttgattt
     3181 agcctctgtt actatttctt catgtactat attacaatta caatcattta tctcttctct
     3241 catttttaat cctccattat tttaatatat gaataactgt tcatatgttc attatatgct
     3301 atcatttcta aatttgcaac aataattttt attaatttaa tataattaga atgcacatat
     3361 tataaacttt tttaatatat atattattag aataagtttt tgtaaacttt tttatagttt
     3421 tctaggaggt gcaagtttaa tatgagtaac aataaaaaaa aggatttaga tactagttac
     3481 atgccttact tagacccagc gaactatgca gattatatta ctaatgaaga aattccccaa
     3541 gatgaattga gaaatgaccc tcaaatttct aatggttata caaaaatacc taataaccag
     3601 aacataaata acctatatcc taataatgta aatcattatc ctaaaagtag taattctcca
     3661 aacaacttgc ctaacacaaa taatacatcc ggaaatatga attacaatac atctgctaca
     3721 aataatatgc ctaataacat gaataataat acgggtatgc ctaacaatat gcctaataac
     3781 atgaatagta acatgggtat gcctaacaat atgcctaata acatgaatag taacatgggt
     3841 atgcctaaca atgtgcctaa taacatgaat agtaatatgg gtatgcctaa caatatgtct
     3901 aataatatga atagtaatat gggtatgcct aacaacatgt ctggtaatat gaataatatg
     3961 tatggtgata caggtatgcc taataacatg aacagtaata tggggtataa tatgaataat
     4021 actatgggta ttcctaactt ctcatgtaat caaaatatgc ctcctaatgt acttatgatg
     4081 ataccaggag ttatatgtca taatacaatg caaggtatgc ctgtagttat gcctagtact
     4141 atgcctccta atatatatac tactccttat ggttctagta atatgtctac acaaggtata
     4201 ccacagacta ctaatattga agaatttgat gaggaagaaa tgtaatatat atttttttaa
     4261 gcaagttgta taaaacatta ataattgacc ataataataa atgtattcct cataatattc
     4321 ccccaatatt attataggta tagaaagttg actgaatata cgcttatttg gtcaacagat
     4381 attaacataa tctaatagga taaacataga aaaagtcaga taaaattaaa ttttatctga
     4441 ctttttcatt taaaaaacac tctcttgtgt tgataggtta ggatggtatg gtactcgtat
     4501 ttgaaactaa tattggcaaa caacataaag aaaagtataa tttttttata ataaactgta
     4561 taaaatttta ataattgacc acaataataa atgtattcct cataatattc ccccaatatt
     4621 atagaaataa atttaagcga gattaaattc agttaatctc gcttaaataa tgtctaaatt
     4681 tattattcca aaaaatcatc aaaatctaat gaacctgatt ctgcaaagtc atataatgca
     4741 ttttgttcaa agaaaaattt ctcttcaaat ttaatatagt cactataatt agattgactt
     4801 atttcattga tataattgca atcattttcg ttataagtac caataaaaaa cttatttccc
     4861 attaagtaat atatagcttt catatcatta tttattatat atacattttc ttttacatgg
     4921 tcattattaa aattaaatct agtaaaaaat ataccttcat tcatattact ttttaacaaa
     4981 aatggatttt ctctataaaa tactactgca aattcttcag gtgaatttac actgtataca
     5041 cttacaaatt cacttttaga aatctcatca aatatctcaa aatcatacat aggttcttta
     5101 tctgtcacta atagagaatt aattctaaaa ccagtttcat aacacttact tatgcaaaaa
     5161 gcaattttac atatataata tccatcattg tcttcatata aagcttcact tatatatctg
     5221 ccttgacctt tatcacttac cacgttttta agtaatgttc catttatatt agtaatatgc
     5281 atatttgcaa tctcgtcact tccagaaaag tactttaaac tttctctatc ccatgctata
     5341 aatctcatgg tcatatagtt tataaattca acttcctcat caactctttt acatatttta
     5401 ttgtataatt tttctatagt taaatctgtt ttatagtttt ttataatatc aatatactca
     5461 catttatctc ctggcaatag cttatcataa taaagattct tattaccaaa ataagatact
     5521 aaaaataaag attcatcctt cgttaattca actctatctg aaccaaatcc tcccatcaat
     5581 ctttcttctt ccatataagc ttcttcttgt gttgggttat ttaaactcac ataatctgct
     5641 aatcctaatc cctctgcatc taataaaaaa tattgacaaa agtgattgtt ttcatcatct
     5701 atccaattta ttataagacc taaactgccc ataagtctag aatttgtagc ctttgcaaaa
     5761 ttaaaattca tatagtcaca tcctttaaac tctaaaatac ttgtttttta atatataaat
     5821 tattatacat atattttaac aacttagata caatttataa tataatataa ggaaatgatt
     5881 aattgaggtg gtcttatgaa aaatatagca gctttttttg atatagatgg tactctttat
     5941 agagactctc ttatggttga acattttaag aaattaatta aatacgatat aattgaccaa
     6001 aaaaattggt ataaacatgc tagagacact tttatggatt gggataaaag acaaggtaat
     6061 tatgatgatt atctattaga aatatgtgac ctttatgttg attcattaaa aggattagat
     6121 aaaacctgta tcaactttac aagtgaccaa gtaattaaat taaaagcaga tagagtttat
     6181 aaatacacac gttcaaggat tgagtggcat ctaaatcaag gacacatagt tatatttata
     6241 tctggtagcc ctggatttct tgttgaaaaa atggcaaaaa aatataatgt aaccgatttt
     6301 ctaggaagtg attatgtctt tgaaaataat atatttacag gcacagttat tccaatgtgg
     6361 gattctataa gtaagaataa tgctataaac gattttgttg taaagtatga tttagattta
     6421 tcaaactcgt atgcctatgg agatactaat ggtgatataa atatgctaaa aagagttgga
     6481 aatccaatag caataaatcc aacaaaggaa cttttatctc aaatatcact tgatgaggaa
     6541 atttctaaaa atgttgagat aatagtagaa agaaaagata ttatttattc tctatctaca
     6601 gatgtaaata tagtggatat ttaatttata ctatctattt taaaaactta tattatatct
     6661 aatatttata tctaacatat aaaagctttg acta
//
LOCUS       DAELJO010000194         6584 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELJO010000194 DAELJO010000000
VERSION     DAELJO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.196"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(619..1179)
                     /locus_tag="KPB88_003261"
     CDS             complement(619..1179)
                     /locus_tag="KPB88_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0055500.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="KPB88_003262"
     CDS             complement(1225..2208)
                     /locus_tag="KPB88_003262"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0055501.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="KPB88_003263"
     CDS             complement(2221..2583)
                     /locus_tag="KPB88_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0055502.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="KPB88_003264"
     CDS             3826..4473
                     /locus_tag="KPB88_003264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055503.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="KPB88_003265"
     CDS             4748..6385
                     /locus_tag="KPB88_003265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055504.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAELJO010000195         6554 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELJO010000195 DAELJO010000000
VERSION     DAELJO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.197"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            447..1361
                     /locus_tag="KPB88_003266"
     CDS             447..1361
                     /locus_tag="KPB88_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055505.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            1385..2020
                     /locus_tag="KPB88_003267"
     CDS             1385..2020
                     /locus_tag="KPB88_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG0055506.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAGGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            2396..3112
                     /gene="pyrF"
                     /locus_tag="KPB88_003268"
     CDS             2396..3112
                     /gene="pyrF"
                     /locus_tag="KPB88_003268"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG0055507.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            3138..4181
                     /gene="carA"
                     /locus_tag="KPB88_003269"
     CDS             3138..4181
                     /gene="carA"
                     /locus_tag="KPB88_003269"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0055508.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            4234..>6554
                     /gene="carB"
                     /locus_tag="KPB88_003270"
     CDS             4234..>6554
                     /gene="carB"
                     /locus_tag="KPB88_003270"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG0055509.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         2739 a          670 c         1277 g         1868 t
ORIGIN      
        1 aaaatatttt aattaaaaca aattatgagg ataacaaact tatttaagca gtatgttttt
       61 tgttgataat aatattaaag tagaaataaa aatacttttt tagtgaaaag ctatacaaga
      121 ataccgaaaa aaacttataa tataataatg agatagatat ttataaatga aaaaaaattt
      181 ttctggcaaa gcttaattaa aaaggtaata gctagtgttg ttatttaaat tagcatagaa
      241 aatcaaataa aaaaattatg ctaatgtata gacattgata aggtagaatt taagcctttt
      301 gaatgctata ttaaactaga gtagatattc ttatgaattt caaaataggg caggaggaaa
      361 aaggaaataa aactataaaa aatataaagt atttatataa atttatagag tttatacgtc
      421 agaagatttc agttaggagg gcataaatgg agctttatga agccattttt tatagaaaat
      481 ctgtaagaaa tttttccagt aagaagataa aaaagccatt gatggaagaa ataaaaagaa
      541 gttgtaaaaa tttagaatac ttaaatgaag atttaaatat taaagcacat gttgttgata
      601 gaggtcatat aatacatttt ttgatgggta aggaatgtaa agtaaaggca cctcattata
      661 tagtagttac atcagacaaa ggtgaggact atttacagaa tataggattt gctacagaag
      721 gtgtagtttt gcgccttaca acattgggaa tagctacttg ctggttggaa ggaaacttaa
      781 agagagaaga tatcttggaa tttgtagata ttgggaataa gaattttgaa gaagatgagg
      841 aagatgattt agattatcta gctggaataa aaacagaaga agatgatgaa aacaaaatag
      901 aaaatcctta tataataata gcatttggat atccagcaaa aaatgaagag ttgtttagga
      961 cgagatatgc tagacctgat agaaagccag taaaagatat gataaataag ataaatagga
     1021 atagaaagtg gaaagatatc ttagaattga caagaagagc tccatctgta aaaaattctc
     1081 aaccgtggta tttccataag gatgaacgag gtcttcattt atttgaaaag agacctaaga
     1141 aacactgtga agatatgaat aaggttagct taggtgtagc cttaagacat tttgatatag
     1201 cttgtataaa aaataaaata gatgtttcat atgaaaaact tcctataagg aataaaatag
     1261 gaaaatcata ttttataact gtagtcgagc atgtgaaacc agaagaagaa acccaagaag
     1321 aaaatgtaac tctagaaaaa gaagaatcgc aagatgaata aaattttgga aggggatatt
     1381 ggatatgtat aagtgtggaa aatgtaatct tagaggctgt tctaatggaa atatggataa
     1441 attaatgaag atatgtccaa ctagagaaat tgaactacaa caaaaagcaa agcaacttta
     1501 tgaagaagaa gagaatagaa aaatagctta taatgctgga ttagttgaag caggggggta
     1561 ttgtcaatat actagactaa aagagattat agagtttgct aaaaagtgtg gttataaaaa
     1621 gattggcata gcattttgta ttggactaaa aagagaaatg caaacagtgc aaaaaatatt
     1681 tgaacatcat gggtttgaag tattttctgt tatatgtaaa aatggggcaa tgcctaagag
     1741 tacaattgga ctagaagcat ctcaaacaat atcaaagtgt tcagatgagg taatgtgcaa
     1801 tccaatagga caagctttat ttttaaatga agctaaggtt gaccttaatg taattcttgg
     1861 attatgtgtt ggtcacgata ctttagccat gaaatatatg gaagctccta taacaacttt
     1921 agttgtcaaa gatagagtaa ctgggcataa tccagttgct gcaatatata atgcagaatc
     1981 atattttgag aaaaaattgt ttgataaaga tgaagaataa ttcaaagact tattgacata
     2041 aaaagcaatt agtggtttaa ttataaaaaa gataaaactt taaattatcc tatagataca
     2101 taaagttata taaataaaga aaaatattac agctaaattc cttaaagtag tccagagaga
     2161 ctaaagggtt atagataatg ttagtatata acctctagta aatcttctag aggtttttga
     2221 tgagtataaa ataaaaattt aaatataata aagtctttta aactagtcca gagagactgg
     2281 gaaagatatc tcatagctaa tgaatagagt tatgtataga taaaatcctt atgcctcttt
     2341 tggtgtgagg atttttttat gattatttag gctttattaa aaaggagatg taaaaatgat
     2401 aaatggtaaa gaaaaattga tagttgcaat tgatactgac gaatttgata aagctaaaga
     2461 actaatagac agattagaag atagtgtaga catatttaag gtagggctag agcaatacgt
     2521 tgccacaaaa ggtaaaacaa tagattatct taaagaaaaa ggtaaaaaaa tatttttaga
     2581 ccttaaattc catgatatac caaatactat gaaaagtgca gtgaaagctg cagtgagaga
     2641 taatgtttgg ttaatgacaa tacatgtatc agatatggaa ggaatgagac aatgtgcact
     2701 aattgctagg gaagaagcag aaagattaaa tatagaaaag ccattagtag ttggagtaac
     2761 agttttaact tcattaagca accaagattt acaggacata ggatgtaata tgacaacaga
     2821 agagttagct ataaagagag ctaaactggc aaaagaatca ggaatagatg gggttgtttg
     2881 ttcagcgcaa gaagtagata aaatagtaga agcatgtgga actgatttta taacagtttg
     2941 cccaggaata agacctaaat cagcagaggt tggagaccaa aaaagagttg taactccatc
     3001 agatgcaata aaaaaaggtg ctcattattt agttgttgga agacctataa caaaagcaga
     3061 aaatccaaga gaatcagcta ttaatattgt aagagaaata gaaaatgctt agataaaata
     3121 tttgggaggt agcttatatg aaggggaaat taattttaga agatggaaca gtatttgaag
     3181 gaaaagcatt tggatatcta aaggaagcta ttggagaagt tgtatttaat acttcaatgg
     3241 ctgggtatgg agaactattg acagatgcaa cttattatgg acaaatagtt actatgactt
     3301 atccacttat aggaaactat ggaataaatt tagaagatgt tgaatcaaat aaggtgcaag
     3361 taaaaggact tattgtaaga gaaaaaagtg ataacccaaa taattttaga tgtgaaatgg
     3421 atatagacac atatctaaag caaaataagg taataggact tgaaggaata gatacaagag
     3481 cattaactaa aattattaga aacaatggaa ctaagagagg aattataact ttagaaaatg
     3541 tatcattaca agatgttcag tctaaattag aagagcattg taacaaagat tcagtaaaaa
     3601 atgttacaag aaaagaaatt gaaaatataa aaggtactgg agctaaagtt gtagttatag
     3661 actttggtgt taaaaatagt gtactagagt ctcttaaggc ttgtaactgt gatataacta
     3721 tagtaccagc aatgactaca gtagaagaaa ttttaaatat aaatcctgac ttaatcttat
     3781 tatctaatgg cccaggaaat ccagaggata taaatgatat agttgaaaat ataaaaaaat
     3841 taataggcaa aaagccattg acaggaatag gtttaggaca tcaaatttta gcattggcat
     3901 taggaggaaa gacttcaaag cttgtatttg gacatagagg aggtaatcac ccagttaaat
     3961 gtttagaaac tggaaaggta tttataactt ctcaaaatca tggatataat gtttcagaaa
     4021 tgccagaaaa tacagaagta actcacataa atttaaatga caatactata gaaggaatga
     4081 gacataagga attaccaata tatagtgttc agtacattcc agaatacaca acaaaagaag
     4141 atttagattg tatatttaat aaattcttag cattaatata gatttagtat tatttacaga
     4201 tagtaaaaac catttagata aggagaaaaa tttatgccta aattagatag tataaagaaa
     4261 actttagtat tagggtcagg accaataata ataggtcaag ctgcagagtt tgactattca
     4321 ggaacacaag cttgccagtc tttgaaagag gaaggaattg aagttgtatt aataaatagt
     4381 aatccagcca ctataatgac tgataaagaa gtggcagata aaatatatat agaaccatta
     4441 acaatagaat ttatagaaaa aataatagaa aaagaaagac cagacagttt acttgcaggt
     4501 atgggtggac aaacagggct aaatctagca gttgaacttt atgaaaaagg catactagat
     4561 aaatatggag taaagataat aggtacttca gtagaatcta tcaaaaaagg tgaagataga
     4621 gatatattta gagaagtaat gaaagaaata aatcagcctg taatagttag tgatatagtt
     4681 acagatttac aagcaggtct tgattatgca cttacaattg gttatccagt agttgtaaga
     4741 ccagcataca cactaggtgg aactggtggg ggtatagctg ataatgagga agaattaaga
     4801 gaaatacttt cacatggtct acaattaagt cctgttggac aagtattaat tgaaaagagt
     4861 ataaaaggtt ggaaagaaat agaatatgag gtaataagag attcaaaggg aaattgtata
     4921 gttgtatgta acatggaaaa tatagaccca gtcggagttc atacaggaga cagtatagtt
     4981 gttgctccaa ctcaaacttt aagtaacaaa gaatgtgcaa tgcttaaaaa agcatcttta
     5041 gatatattaa atgcagtaga agttcaaggt ggttgtaacg ttcaatttgc acttaatcca
     5101 catagttttg aatatgcagt tatagagata aatccaagag ttagtagatc atcagcttta
     5161 gcatcaaaag ctacaggata tccaatagct aaagtttcat caaaaatagc tttaggttat
     5221 actttagatg agatagaaaa tgcagtaact aaaaaaactt atgcttgttt tgaaccaact
     5281 atagattatg tggtggcaaa aataccaaaa tggccttttg ataaatttaa aaaagcaaat
     5341 agaaaattag gaactaagat gatggcaact ggtgaaatta tgagtatagg aagtaatttt
     5401 gaggctgcta ttcttaaagg gataagatca cttgaaacag gaaaatattc attagttcac
     5461 acaccatctg aggagagaag cattgaggaa ttaaagaaaa gagttgtagt acctgatgat
     5521 gagagactat ttgatttagc tgaaatgata agaagaggct ataaagtaga aatgatagaa
     5581 caaattacag gtatagataa atggtttata aataaattta aatggatagt agagcaagaa
     5641 gaaaaattaa aagttatgaa aattgaagat ttaactaaag actatcttct tgaattaaag
     5701 aaaaagggat tctctgataa aggtatatct gacttaatga agataagtcc agaaaaatta
     5761 tatgaattga gaagtctata caatatcaaa ccagcatata aaatggttga tacttgtgca
     5821 ggtgagatag atgctatatc tccttactat tattcaactt atgagcaata tgatgaggta
     5881 gttgttagtg ataagaaaaa ggtgatagta ttaggttctg gtccaataag aataggtcaa
     5941 ggtatagaat ttgactattg ttcagttcac tgtgtaaaat ctttaagaaa aatggacata
     6001 gaaactataa tcataaacaa taaccctgaa actgtaagta cagactttga tacatcagat
     6061 aagctgtatt ttgaaccatt aacagaagaa gaagttttaa gtataataga aaaagaaaag
     6121 ccagaaggtg taatactaca gtttggtgga caaacagcta taaaattggc taaattttta
     6181 catgaaaaaa atatacctat attgggaact gattttgatg atatagatgc agcagaagat
     6241 agagaaaaat ttgatgattt actagagaga ctagatataa atagaccaaa aggaaagggt
     6301 gtatggtcac ttaacgaagg tattgaagtt gctaatgagt taggttatcc agtattagtt
     6361 agaccatctt atgtacttgg tggtcaaggt atggaaataa cttataacga agaaaaatta
     6421 tctcaatacc tacaagatgc atttgataga gaccataaaa atccagtact tatagataaa
     6481 tatctaactg gtagagaaat agaagtagat gctatatgtg ataaggaaga tatattgata
     6541 cctggtataa tgga
//
LOCUS       DAELJO010000196         6503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELJO010000196 DAELJO010000000
VERSION     DAELJO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.198"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            947..2305
                     /locus_tag="KPB88_003271"
     CDS             947..2305
                     /locus_tag="KPB88_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0055510.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2412..3206
                     /locus_tag="KPB88_003272"
     CDS             2412..3206
                     /locus_tag="KPB88_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0055511.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3199..5505
                     /locus_tag="KPB88_003273"
     CDS             3199..5505
                     /locus_tag="KPB88_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0055512.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVVAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5608..>6503
                     /locus_tag="KPB88_003274"
     CDS             5608..>6503
                     /locus_tag="KPB88_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0055513.1"
                     /translation="MSSRVYSKGGEFMSNRIKNIHYGTQRQQLKNLTKKEYQALREMC
                     FLAKNLYNVGTYNVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSS
                     FRSFFGLIKLAKEGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKK
                     LYGKVSIKVPQNILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLG
                     LENLCTCVTNLGDSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNK
                     RNNQVKDYVNKTCFYIIDYCVK"
BASE COUNT         2498 a          723 c         1248 g         2034 t
ORIGIN      
        1 tattttatgt tgcagataca aatatagtaa aggagagtac attggtacaa ctgagataca
       61 tagcttatta agtttttctg tcttatgaga attatattat aaaatattca taaatatttt
      121 gagtttaaat taaataatta ttttatgctt agattaattg atatttaatt aatttaataa
      181 caaaaacagt gaaagagtaa ataatattat ttacaggggg taaagcaaag tatgttaaat
      241 aaattgtact tttggccccg tacatagaga aatctatgta ggacacaact ttaaaaccag
      301 taaatcctaa agccttgcaa ctacaataaa agatagaaag aaacttttat gaatgtggtg
      361 aaaaccagaa acaatagtga ggatggtata tggtgagagc ctaagtactt aaaaatggac
      421 atttggtcgc caagcctaga aataggaagg tcaaacgact atctcgagag agagtacact
      481 ataagcgatt gatagtggaa acgggttgct cttagcaggt aatgctgaag atgaagaaat
      541 agtctacgct catatgaaag tatgaggtca ctggatttga agtgacatct ataaagtagc
      601 gtttataggt aaatgtttaa aacttgaaac atgtaaatac ttatgaaaac ttataactta
      661 taatgattaa gattgaaaga tttaaattga attaaattta ctataaaaaa ttaaaaattc
      721 atagctataa tactcttata tttgtaaagc atggtatata atgtatacaa aattaaagaa
      781 tacgtttatt tgttaataat taaatttatt gcatctgttt tttaagacta aaaacttaaa
      841 tatatgttgg agtatagttg atatttatga acatcattaa tttaatatat tagattttta
      901 agtatttata taattcaata agttttatgt aacgggagtt tttattatga aagcaaaaag
      961 tacatcaaaa tttgaattag acggaatacc accactaaag gaagcaatac ctttagggtt
     1021 acaacactta atggcaatgg taataggtag ttgtttacca gcgatattga ttgcaaatgc
     1081 agctggactt aatcataaga tgtcaacatt attgattcaa gccgcattag tttttgcagg
     1141 attatcaact ttattacaat tatatccaat acctttgttt aaaggtttta aattaggagg
     1201 aggaatacca gttataatgg gagctacagc tatgtttata ggagctgggg tatctgttgc
     1261 aagtaagtat ggactaccag tattatttgg ctctcaaata atagctggta tagtaataat
     1321 tataattggt tttggtctta aaaaaatacg atttatattt actccagctg tatcaggaac
     1381 aatagttgca tgtatgggtc ttggactttt tccaatagct ataaaaaact tagctggggg
     1441 tatgggcaat gagacttttg ggaatccaat taattttata gttggaatat ctgtatctct
     1501 tatgatatta ggattaaata aatatggaaa aggattattt aaagatgcat caatattagt
     1561 tagcataatt tttggataca ttttatcatt aattttgggt atagtaaatt tttcttcaat
     1621 tcaggagttt actttggttg cacttccaaa accacttgct tttgggctag atataagact
     1681 tgaagttgtt gtaatgtttt caataatata tttagttgag atagctgata taatgggtgc
     1741 ttgtacactt tctgcggtag gagggcttaa tagacaagta acagatgaag aactttcttc
     1801 tgctgtttta ggaaatggag tactctctac aataggtgca ttattttctg caactccaat
     1861 ggggatgttt ggacaaaatt ctgcaatcgt ttccaataca aaggttgtaa gtaaatttgt
     1921 actagctatt ggaggaattg gattattttt ggcaggaata tctcctttat tagctaattt
     1981 aataagaaca attcctccct gtgtggttgg aggagcaaca ctcgttatat tttcaactct
     2041 tacaacatct ggtttaagac ttgttagtat ggatggattt aatcaagaaa atagcatgat
     2101 attaggtctt tctatggctt ctggtattgg attcatggta gctcctcaag ttcttgaaaa
     2161 atttccaaaa tttatagaga cattacttgc agattcaagt gtagtttcag gagcaatggt
     2221 agccataata attcaagctt tatatatggt aaaatttcct ggaaataaaa gtgataaggt
     2281 tgaggataaa aataaaagtt tataatgtaa aggtttaata tatgtgaatt aaatatacta
     2341 caattataaa tataaataaa agatttatta aaaacttttg tgttttaata agttaatgat
     2401 ggggggataa gatggttact ataaaggaat attcagttcc acagagttta gaagaagcat
     2461 ataaaatttt aatttctaaa aaaaataatg ttattttagg tggatgtgga tttataaaat
     2521 taagtaataa aaatatagga acagcgatag atttaaaaga tataaatcta aattatataa
     2581 aagaggatga aaaaaatatt ttgataggtg cagatacttc acttagaagt ttagaaattg
     2641 ataaaactat aaaaaattac tgtagtggaa tactttcaag cgctgtttct aatatagttg
     2701 gtgttcaatt tagacaaggg gctagaattg gtgcaagtgt attttcaaag tatggctttt
     2761 ctgacttaat accagcgctt ttggttgttg gagcgaaggt gagattatac aataaaggaa
     2821 tactagagtt aagtgagttt ttggatagtg aattaagtag ggatatattg gtcgaagtaa
     2881 tactaccaaa aaaagatgca atagctgttt ttgactcaat aagaaaatgc acaggagatt
     2941 tttctgtatt aaattcagca atgctaaaag aaaatgatac ttacaaaata gcaattgggg
     3001 caagacctca aaaagctaga ttagcacttg aagcgagtaa tatattaaat aaagaaaaag
     3061 atatagataa ggcatctatt gttgctagta aggaacttac ttatggaagt aatatgagag
     3121 ctagtaaaga gtacagaaag gatatggcaa gtgcattagt taaaaggatg tataatgcta
     3181 ttgaggaggg aatgtataat gattaataag gggataagaa agatagatag tacagcaatt
     3241 gtcacaggaa aacctctata tactgaagac ctcattatgc ataaagatgt tttgacatta
     3301 aaactactta gaagtccaca tgctcatgct aagataaaga atatagatac atcaaaagcc
     3361 gaaaaaattc caggggttgt gggtatttat acgtataaag atgtaccaca ggtcagatat
     3421 tgtgttccag gtcaaacaga accagaagca tcaccttatg atagagtttt acttgaagat
     3481 acagttagat ttattggaga tgaagtagct ttagtggttg ctgaagatga gaaaacagca
     3541 cttaaagcct taaaaatgat aaaggtagat tatgaggttt taaagcctgt gtttgatgca
     3601 agagaagcag aaggaaatga agttgttata catccagaaa aagacatagt agagccatac
     3661 ccttttggat tggatgccaa aaataatata gtatcaaaag aattttttga atttggagat
     3721 gttgaagcta agcttaaaga aagtgatttt gtggttgaat cttcatacaa aacccaaagt
     3781 caatcgcatt gcatgatgga aactcatagg tcatattcat ataaagatat aaatgataga
     3841 attgttgtag tttcatcatt acaatcacca cacaatgtaa agagaatact tagaaaagta
     3901 ttgggtatac catcttcaaa aataagagta attaaaccta gaataggtgg tggatttgga
     3961 ggaaagaata tagcagcaac agatatattt actgcatttg ttactatgaa aactggtaga
     4021 ccagcaaaat ttgtatttac aagaagtgaa acttttgtag gttctaatag tagacatgaa
     4081 atgttttttg atgttaaagt tggtgcagat aaagaaggaa atttaaaagc aatagagcta
     4141 aagtcattaa acaatacagg tgcttatggt gggaatggtg tctctgtttc ttccgaatct
     4201 ggacacaata ccttaccaat atataatgat attgatgctg ttagatttaa tgctagaaca
     4261 gtatatacta ataggttacc agcaggagca cttagagggt atggtgctac acagggaata
     4321 tttgcaatag atagtgctat tgatgaattg gcacataaga ttggaatgga cccacttgaa
     4381 tttagaatta aaaatataat agataggtat accaatggaa aagcagctga agacgaaata
     4441 ttaagttgtg agctgagaca atgtatagaa aagggcaaga aattaattaa ttgggatgat
     4501 aaatatccat gtaaagatat aggaaacaac aaagtaagag cagtaggtat ggcagtagca
     4561 aatcatggtt ctggtatacc gcgtgtagat atggcaacag tcactataag aatggatgaa
     4621 gatggcacat ataaactatt atctggtctt gctgacttag gtcaaggggc tgatacaatt
     4681 caagctcaaa ttgctgcgga agctttaaat actactgtag atagaatttc tatttataca
     4741 ggtgattcag atttatgtcc ctatgataca ggagcatttg cttcttgtac aacaacggtt
     4801 acaggaaatg caacaataaa gacagcaaat aaattaaaag atatattact ttcagttgca
     4861 gagtcaaagc taggatttaa aaaagaagat ttagagttgg gaaaagacag ggtttttgtt
     4921 aaaaatgata atagcaagtt tgttctatta gaggaattgg gtagagaatc tgttggagga
     4981 ataggatttg ctagtgatga tgtaccagta gttttggaag caacagaatc ttttggcatg
     5041 ttgaaaaaaa taaaaccatt tatagcagga tttgcagaga tagaattaga taaaaataca
     5101 ggcgaatata aggtcataaa ttttgcatgt gtacctgatt gtggaacagt tataaatcca
     5161 actttagcta gaatacaagt agaaggtggt gtgatgatgg gtatagggtt tgcaatgtat
     5221 gaagacccta tatttgataa agatgggaga ttattaacag atagtttcct acagtataaa
     5281 gttcctacaa aaaaagaagt tggaaatata ttggtagatt ttgctgagaa tttcgaccca
     5341 ggaggtcctt atggtgccaa atcattagga gaaatagtta tacatacacc agcaccagct
     5401 atagcaaatg caatatttaa tgctacagga gttcgtttaa gagagcttcc tatgactttt
     5461 gaaaaagttt atatgggtat gaaaaataat aataaacaat tataataaat aataaggtgt
     5521 ataaaagcaa gtaaatttaa attattttgt aaaataaagt aattaaatgg acattatata
     5581 tggtgttttg taaaaatacc gtatataatg tctagtagag tatattctaa aggaggtgag
     5641 ttcatgtcaa acagaataaa aaatatacac tatggaactc agagacaaca attaaaaaac
     5701 ttaactaaaa aagaatatca agctctacga gagatgtgct tcttagctaa gaacttatat
     5761 aatgttggta catataatgt aagacagcat tatttcaatc aaggagaaca tcttaactat
     5821 gaagctaatt atcatgtatg caaagaaaat gagaactata agcttctcaa tagtaatata
     5881 gcgcaacaaa tattaaaaga agtagacagt tcgtttaggt cattttttgg gttaattaaa
     5941 ttagccaaag aaggaaaata tgactttaga gggataaaat cacctaaata tttagataaa
     6001 gaagggtttt ttagcataat aataggtcaa ataagaatca aagaagatgg gattttaaat
     6061 gtacctatgt ctccagcatt taaaaaacta tatgggaaag taagtataaa agttccacaa
     6121 aatatactag agaaaaaaat taaagaaata agaataatac ctaaacataa agctaggttc
     6181 tttgaaattc agtattgtta tgaaatacca aaatcagagg aagtttcaaa tcaaaaaaat
     6241 gcactagcta tagacttggg tttagaaaat ttatgtacat gtgttaccaa tttaggtgat
     6301 agttttatca tagatggaaa aaggctaaaa tctgtgaatc agtgggcaaa caaacagaat
     6361 gataaaatag aagccttaaa attaaaaaag aatataactg caataacaag aaaacaattt
     6421 aaagtttgga ataagagaaa taatcaagtt aaagactatg taaataaaac ctgtttttat
     6481 ataattgatt attgtgtaaa aaa
//
LOCUS       DAELJO010000197         6457 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELJO010000197 DAELJO010000000
VERSION     DAELJO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.199"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..456)
                     /locus_tag="KPB88_003275"
     CDS             complement(<1..456)
                     /locus_tag="KPB88_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HBG0055514.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQRKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDE"
     gene            1539..1943
                     /locus_tag="KPB88_003276"
     CDS             1539..1943
                     /locus_tag="KPB88_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0055515.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(2200..3195)
                     /locus_tag="KPB88_003277"
     CDS             complement(2200..3195)
                     /locus_tag="KPB88_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0055516.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(3479..4315)
                     /locus_tag="KPB88_003278"
     CDS             complement(3479..4315)
                     /locus_tag="KPB88_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG0055517.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(4803..5366)
                     /locus_tag="KPB88_003279"
     CDS             complement(4803..5366)
                     /locus_tag="KPB88_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055518.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(5464..6393)
                     /gene="glsA"
                     /locus_tag="KPB88_003280"
     CDS             complement(5464..6393)
                     /gene="glsA"
                     /locus_tag="KPB88_003280"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG0055519.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
BASE COUNT         2058 a         1173 c          599 g         2627 t
ORIGIN      
        1 ttcatcagtt atgatattat tatttattcc aaaatatcct attcctgata aataagctaa
       61 atacctgtca actaatggtc cattatcagc aaataacttg tatttaaaat ctgttactgt
      121 tttagaaata taatcacaaa tttgttgtaa aaagtccttt actacaatgt gataatcctt
      181 tccatgacaa tattttgata aatttgaatt tttattcttt tcttgtctat ttatatgata
      241 tggaaatgca cagactatta tagagttagc atccttcatt atatgtcttg ggttaattct
      301 gttctcaatt attggttctt ccataccagt taaataacct tttcgttgtc tgtccttaag
      361 tatcttttct aaattatcat acttatctac ccctgctact cctacacagt ttaatcctat
      421 taaattacaa aatgctttta attccttatt atccataaaa ttattccttt ttaatctaat
      481 tgacttttta aatttattta atttttttag tctttttaac cttttctagc tactaactct
      541 tttaaatact taatcttttt agctattcaa ttttcttacc tgcttaactc ttttagatac
      601 ttcgtctttt tatctattta atttttttac ctactaactc ttttaggtac ttcgtctttt
      661 tatctattta attttttacc tactaactct tttagatact tcatcttttt acctatttag
      721 ttttttacct actaactctt ttagatactt catctttttt atttactaac tcttttagat
      781 acttcgtctt tttcatttac ttatttaact tttttatcta cttaactcct ttaaataatt
      841 agtcttttta tctatttaat tattttacct actaactctt ttgaatactt agtctttttt
      901 atttatttaa tttttttatc tactaacgct tttagatact taatcttttt atctatttaa
      961 tttttttatc tactaactcc tttatatact tagccttttt catttactta tttaactttt
     1021 ttacctatta actcttttag atacttagcc tttttcattt acttatttaa cttttttacc
     1081 tacttaactc ttttagatac ttagcctttt taatctattc aacctttttt acccacttaa
     1141 ccttcttacc ttttaatata aactctaaaa cttcaaatat cattagttat attttgacat
     1201 tcttcctata gttaatgcaa aaagattgat tttttcttat aattaatatc ccatttacat
     1261 tgccatttat taagtcttta cttgacttat atggatttct ttttactctc tttactaaac
     1321 ttatacttat taatattatg aaggtcttat aatagtctaa tatgaatatg cccatcatat
     1381 atcaaatttt aaaatttgta agcaaaatac ttatcttata ttactacatc taaagtggaa
     1441 aattttacaa acaagctctg taaaaacact ttaaataaca atttgctttt aaatcatata
     1501 cagattataa taaatactgt ataaggatgg tgaaaaattt gattaaaata acaattccag
     1561 gtagacctat tagtaaatct aattttaagc ttcataacat aaatggtcaa gcatggatgc
     1621 cttcaaaggg aaaatattct aagtatcttg catacgaaaa catgattgca ggttttataa
     1681 accaacagta tcaaggggaa actgttgaag aaaatctaat aacagtcttg aaattattct
     1741 tcccaaataa gagaatgggg gatttacata attatccaaa aagtatttgt gatggtattg
     1801 aaaaaagtgg aataataaaa aatgataaac aacttaagcc agtactttta tttgatttta
     1861 tagataaaga taaccctagg gttgaaattg aactttatcc agtatctaaa tacgatatat
     1921 cctataatat ttttgaaaaa taaagatttt atgtgttatt cgacattata ttgatactcc
     1981 agtatttgaa actatactaa tacaacaata ataattgtgt tatgtctata ctcaaacaaa
     2041 tgtttttaaa aagcattaag taaatattta ataagtatta aacaaatatt aaatttatat
     2101 aatagctata actatgtaaa tttaatattt gtatataaga ccacatctaa taaaaagaaa
     2161 tcggagtaaa tttaatacac cgatttcaat aaataaaatc tatcctagct tttcaacaac
     2221 agtcttaagc atttctctta taggaagttc atatggacat ctactctcgc aaagcccaca
     2281 gtcaatacac tcagtaggtt taacatccat aacctcatat ttttctttag cccactcttt
     2341 taatccatat ctagtataat aaccttcaca taaaaaactc aatggtatat ttattcctac
     2401 agcacaaggc atacaatatt cgcatcttct acagaatttt ttacctaaag aatttcttat
     2461 ttcttcaatt tccttattat ctttttcatc tagcacaaga ttttcaagta cagcgacatt
     2521 ttgtctaacc tgttcaacac tctccatgcc tggtataact acatctatgt aatctttaga
     2581 tagtatatat ttaatagcca gtgttgcatt gtctaaagca cctccagcta aaggtttcat
     2641 tactattatc ccaattcctt tttcatgagc ttttttaaac acttcatctg cttgtccttc
     2701 aactatattg taagggaatt gtatagtgtc aaattttcca tcttctatag ctttttctat
     2761 agtatttaaa ttgtgactag ttataccaat atgttttatc ttaccttgct cctttgcctc
     2821 taaaagggca ctataagcca ttttatcttt aaaaagatta tcatactcct cttctttaac
     2881 attatggaat tgatataaat ctataaaatc agttttaaaa ttatttaaac ttatttcaac
     2941 atctctcttc attgaatcat aatctctgga catactttta gtagctaaga aaaatttatc
     3001 tctttttcct tcaatggcaa ttccgattgc ttcctcactt attgtatacc ctcttgcact
     3061 gtcaataaag tttattcctt gtttttctaa ctcatttata actaagttag tatcatcttg
     3121 agtaatcctt tgtattggaa tgcccccaaa gccaactctc tttattttca tgttagtatt
     3181 gcctaaattt ctcatatctt tccccaccta acttttaata attttttata taatataaat
     3241 atgtcacctt taattataca acaaattatg agaatctttg caatatattt acatactatt
     3301 cataatataa ttatatttta tatcagtcta ttcataatat aatcatactt tatacccagt
     3361 taatttgtat acaatttttg ttattcctat ccattaattt aaattatcac atttcatgat
     3421 tgtatcttca ccctaattat gttgagggcg gagtatttca catattttta ataaaggttt
     3481 atttactaga tagtatttct tttatcattt taagctctct cttgtgacct tcatcatcaa
     3541 aaggagtttc caagaaaaat ggtaaatgtt ttagactagg atgttccata aaatcaatta
     3601 atgcttttaa tcctatcttt ccttccccta taggagcatg tctgtctttt ttgtctccaa
     3661 atggcatcat actatcattt agatggacag ttttcaatct ttctatgcct ataaccttat
     3721 caaattcttc caaaacccca tctaaatcat tcactatatc ataaccagct gaaaaaatat
     3781 gacatgtatc aagacacaca cctatttttt ctttatgctc aattttgtct attatacttt
     3841 ttaattgctc aaatttaaaa cctatttcag ttccctttcc cgacatagtt tcaagaagta
     3901 tagttatatt ttcatcaccc tttatggcat tgttcaatcc ttttactatt ctatctatac
     3961 caaaatctac accaccacct acatgacttc caggatgaaa acacatgtat tcaattccta
     4021 atgagtccat ccttcttata tcctcatcta tcacccttgt tgcaaattcg tatacatcat
     4081 cctttgtacc acccagattc atagtatatg gtgcatgtgc aagcagtggt gcaaaattat
     4141 tttcttttct aatttcttga aatttaatta tatctttttc atcaaattct ttagcatttc
     4201 ctcctcttgg atttctacta aaaaattgaa atgtatttgc accaatatat acagctgtct
     4261 cagccgcatt agcaaatcct tttgctattg aaagatgtgt tcctaattta atcatattaa
     4321 tctcctttta tttgtactat tacttgtact caattttaat tttgaactac tccaccttaa
     4381 ctacatttag ccaatatatg aagatggaga ttcctatttg atagtacttt ttcactaaaa
     4441 attcataagc tctaagggat gttctatccc aactaatctc attcctcttt taacatattt
     4501 ctagcattga tttaatttat attaattttt aattttaata tttacacttt aattataccc
     4561 attttaatca atttcaaata ttataatctt acatactttg tatattatct aagcacttct
     4621 tagatagtgt atacatctta cactatacat attatttaaa aatacaatag gtataaaaaa
     4681 acaaaaaaat agagattaaa aatcattgtt ttacccatac caaaaactat tttttgatta
     4741 ttaagtaaat tttgatgttc aatctctaat attatatctt taattaatcc ataattaatt
     4801 ctctaactag gacattcctc ctcaagagta ggaactataa ccatagctat atcatactta
     4861 gaaccttcaa cattgtgttg tcttttattt aaaatttcat ctatctttga cttcatagtg
     4921 tcactaagtt caactaattc atcatgagta agagaaatat catgataatc aactatattt
     4981 tcattttcta aaacatcttc ctctgctgcc ttataaaaaa cttcactcat attttcaaat
     5041 tttgatatgt acaattcttc tttttcttcc ccattgtcta aagaaaaatt aagtatccta
     5101 ttccctataa ccacattttt tgcacttgga taatagtact tttccacaat ccccgacttt
     5161 attttttcct caactaattc taaaatacca actttgtata acatttttat atggtagttt
     5221 atttttgcat gaggttcttc caataattga gataattgtt ttgctgacaa tggcgatttg
     5281 tcaaatgctt ttagaatatc tattctacgt ggatgcgcaa tagcctttat gcactccaaa
     5341 tctcgtaata ccaatacctc tttcattgac ccctcctaaa aaatttattt aattcatatt
     5401 tctttacaat ttaattatat attaaattat atgttgttgt caaaaaaatc ctttcttaat
     5461 attttaaaaa atatttaatt tgagtttatt tgacaactcc tctaaaactt taactcctgc
     5521 gacgctgtta ccttttttgt caagcgcagg tccataaaca ccaatcccca tgcgtcccgg
     5581 cacagaagcc attataccac ccccaactcc agattttgca ggaatgccaa ctttaatagc
     5641 aaattcccca gaagcatcat acattccaca agtcatcata aatgtcttga caatcctaga
     5701 aaccatttca ctcattaaat gctcaccatt ttctatatct actccataat ttgcaagatt
     5761 tattccaatt ctcgctaaat ctacagaatc tatctcaatt gagcattgtt taaaataaag
     5821 gtctagaaca tcttctacat tccctctgat aaatccatca cttttcaata agtaagccat
     5881 agctctgttt ctatctccag tcattttttc agacttatag acatcatagt ttattcctat
     5941 attatcattt tttgctaact ttctaaagaa actaagcatt ctttcttctt tttcttctaa
     6001 acaactccca tttataaggg aagtggttac tatagcccct gcatttatca ttggattaca
     6061 gggttttttt gtgtcattaa tttcaagttt cattattgaa ttaaatgggt ccccacttgg
     6121 ctccattcca acgtttgaaa aaacgtcttc ccaatcatta tccataagtg ccatagcaag
     6181 cacaattggt tttgaaatac tttgtatagt aaactttgtg ctacagtttc cagcactata
     6241 aagattattt tcgctatcta tgatacaaat tcctaaatca tttcttctag cgttttttaa
     6301 ttctggtatg taactagcga cttgtccata attagtatac ttcttattag aacttataat
     6361 ttcttttaat aatgtctcat ctaacgtatt catatgcacc tcttatatta tgttatatat
     6421 tctattttag cacaagttta tactataata aaacaat
//
LOCUS       DAELJO010000198         6418 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELJO010000198 DAELJO010000000
VERSION     DAELJO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.200"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            148..1008
                     /locus_tag="KPB88_003281"
     CDS             148..1008
                     /locus_tag="KPB88_003281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055520.1"
                     /translation="MYSILSSLTKGEPNLTHLEIKTIYNNLKKQSTKLNDGSLEYSEI
                     TRFVFSVEEEDIEYVFLNIKLLEEYESSNKNELVRKLLNKIKVHIDLEQKRLSFLEKK
                     QTEQLTKISENNIEKFKNIKEEIDSHTNAITNSKQNLENNKREINNLYTNLITILGLF
                     SAIVITFFGGLSTINGILSNMDSISKYRLIFIILLMMFAMFNIIFLLLYYVSALTGKR
                     ISKNCEQNCEIYKKQRAEGNNHRSNNKWIECKNKKILCSKQRYPLIFYFDLLNIILLL
                     LVCIAYYLKI"
     gene            1651..1827
                     /locus_tag="KPB88_003282"
     CDS             1651..1827
                     /locus_tag="KPB88_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0055521.1"
                     /translation="MEQRELNISFHKSGNGYTTTRLSLPINWVKELGISQDERKVIVT
                     LEDGKIIIEKAENE"
     gene            complement(1872..2678)
                     /locus_tag="KPB88_003283"
     CDS             complement(1872..2678)
                     /locus_tag="KPB88_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0055522.1"
                     /translation="MKVVIVPGHTLTGKGTGAVGYIDEGKENRILNDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCEIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNKKLSIIFKNRGAKSDARGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGAVDKIPATVVGWGYNDGKILICDIK
                     DYIPGQTENLYVVGGGACEKIGSMTKENYTMIKGNDRFDTLYKALDFINK"
     gene            complement(2678..2944)
                     /locus_tag="KPB88_003284"
     CDS             complement(2678..2944)
                     /locus_tag="KPB88_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055523.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKVMLQKEGDK"
     gene            complement(2991..3161)
                     /locus_tag="KPB88_003285"
     CDS             complement(2991..3161)
                     /locus_tag="KPB88_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055524.1"
                     /translation="MYNILKRMIEQKNYETKEELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTPIEPII"
     gene            complement(3187..3504)
                     /locus_tag="KPB88_003286"
     CDS             complement(3187..3504)
                     /locus_tag="KPB88_003286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055525.1"
                     /translation="MNKIKFRIIREHNFSKEEIDNIGIDNFFIADNGQLWTPLISKGE
                     IIKTGEQSYNEWLELQINPVKPEQSLDRGTILEENQKEQDNLLIDNAYRIAILELNTN
                     NVL"
     gene            complement(3507..5837)
                     /locus_tag="KPB88_003287"
     CDS             complement(3507..5837)
                     /locus_tag="KPB88_003287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055526.1"
                     /translation="MLNEDVVKNINKLNNEQDNINLELSKKANKEDLEKLIQGGPNVS
                     VSKDISINNWILEENVYTAIVEHNLVTRKVLVSLIDKSTNNNVFCSYQILDDNSIKVF
                     NELNNELECIVVNGNSAINMISATIDDNRSTETTTYSSVKIKALLEVLENKINDIENL
                     KEIDNIKCSTDTGEYTIENSKKGYLTNFNIEGKTLIDLWGKTSSDFSLWKATFVDGKI
                     NILTENDIRYSNFFTINYTSYKPDTIYTIIVDVDKNTLPSTSGIYIHSLGEENSVFIP
                     NLTNIAIPGGVIGKFKYTFTTISDLSDCNAVLRSVLDNDTLTSGYEVSLKITILEGDY
                     TDINIDYSNELLSVGQRDKIEFLSYQYSGINIFNKNADFKDNYILQYLNGEELISEAS
                     NHKYTLDYIEIEPDTEYTFYNCSRNICWYDINKSFIPAPLNERIIGDKDIFYVARSPK
                     NAKYLRVTIIKDLHDNGNKTIITKGNKYDKKTIPHTLRGLPNGIRDEIVYKNGSYKLI
                     KRCEEIVFNGNENWIEVHGMYEFRLNLDISVLRLDMSKFITNLYKQSHVDYIFNVQTE
                     SALSISTENGIGVIDIVDTSISPFSIANFKEKLKKNPLKIIYELEIPREVELGLLNLE
                     QYDNQTGFICSSGIAIPNVSFESTRNLGSHIEVIRENLKDITTLTESNVDFKFINGWD
                     KVWQSATITKVGKYLFVNMIVTKSTSSTVLEEAIIKLPLGISRQNYSCPVMNSSCEQV
                     DTVGIACVNNTILMLTPGHDITDKGILISVIVNLEV"
     gene            complement(5862..>6418)
                     /locus_tag="KPB88_003288"
     CDS             complement(5862..>6418)
                     /locus_tag="KPB88_003288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055527.1"
                     /translation="IGVNTIINNSGFLNIVISSSTAMSAIAGSSTAMSAIAGSSTAMS
                     AIAGKPVAISAIIKSTMACSKIDSKIQSYRSTLVNSLNNASYFTKTTEISTNSNGNGT
                     TLDRTLYTGNKIIIPTQFRDKGSGANTITLYSSSTNIQIYNTTITQTTVSITSGVALR
                     GVRELHGTYFPNENEITYDVYTAR"
BASE COUNT         2155 a          955 c          747 g         2561 t
ORIGIN      
        1 ttataaaaat tatactcctt gggacatggt taaaaaaaca catgaagaaa aaccttggaa
       61 aaatacagta aaaaatgaaa ctataaaaac tcaatccata gcagagtatt ttttaaataa
      121 tccagatgaa atatatggag gacaatagtg tatagtatat tatcaagttt aacaaagggt
      181 gaacctaatc ttactcactt agaaattaaa actatctaca ataatttaaa aaaacaatct
      241 acaaaattaa atgatggaag tttagaatac tcagaaatta cacgattcgt tttttcagta
      301 gaggaagaag atattgaata tgtatttctt aatattaaat tattagaaga atatgaatca
      361 tctaacaaaa atgaacttgt taggaaacta ttaaataaaa ttaaagttca catagactta
      421 gaacaaaaaa gactaagttt tttggagaaa aaacaaacag agcaattaac taagatttct
      481 gaaaataata tagaaaaatt taaaaacata aaggaagaaa ttgatagtca taccaatgcc
      541 attactaatt ctaagcaaaa tttagaaaat aataaaagag aaataaataa tctatacact
      601 aatttaataa ctatattggg attattttct gctatagtta ttactttttt tggtggtcta
      661 agtacaataa atggaatttt aagtaatatg gattcaataa gtaaatatcg tctaatattc
      721 ataatattat taatgatgtt tgctatgttc aatattatat tcctattatt atactatgta
      781 tctgctttga caggcaaaag aatatctaaa aactgtgaac aaaattgcga aatttataaa
      841 aaacaaagag ctgaaggtaa taatcatcgt tctaacaata aatggataga atgtaaaaat
      901 aaaaaaattt tatgttcaaa acaaagatac ccacttatat tttattttga tttattaaat
      961 attattctat tgttattggt ttgtatagct tattatctta aaatataaat taaatactat
     1021 gttgtcttat ttattcaata gactatggat attaaatcta tagtcttttt tatatattaa
     1081 atcaaaattt gttatcttta accacttatt tacatataag aagctccaat ttattcaata
     1141 ctacagtaat catagtaatt attaaattta ttgaaatatg tagataaaag tgtcgtatat
     1201 tacgcaccgt aatattataa tatatttata cacaaggatg tgaaaaaata aaaaactaag
     1261 aaaggaggac aaagaaagtg gggatattag aaagaagaaa aaataaaaaa gagaacaaat
     1321 ttaatataat taaaaaagtt ctctctttta tattattaat actaaatata gctcttacag
     1381 ttttaagact aattaaacaa ctataagagt taagcctaga aggaaatgca gttccttcta
     1441 ggtgccttct ttctaatatt ataacatact ttctaaaatg ccatgaatag aaatttttac
     1501 aaaatatgtg ttgctgttgt tttaataact atagtgtttg atgtaatact cttaataaaa
     1561 agttttgata tatggaatat tattggatta ttaataagta tctcactatt agttttcttt
     1621 acaaaagaat taaaaaggga tggtaattaa atggaacaaa gagaattaaa tataagtttt
     1681 cataaaagtg gaaatggcta tactactact agattatctt tacctattaa ttgggttaaa
     1741 gaactaggta tctctcaaga tgaaagaaaa gttatagtta cacttgaaga tggaaaaata
     1801 attattgaaa aagctgaaaa tgaataaaaa aagaggtagt aactatttcc agttactacc
     1861 tcctaatcga tttacttgtt tataaaatct aatgccttat aaagagtgtc aaatctatca
     1921 ttacctttta tcatggtata attttcttta gtcatggaac ctatcttctc acatgctcca
     1981 ccaccaacaa catataaatt ttctgtctga cctggtatgt aatcttttat atcacatatt
     2041 agtattttac catcattata tccccagcca actacagttg caggtatttt gtcaactgct
     2101 ccatcataaa cgattgtatg tttgtacatg atttttccct caccattctc tttattatct
     2161 attgtcttat ttaaaatacc ttctgctatt aatttagcaa ctatgtcttt atgtctaata
     2221 taataatctg tatctgcttt actatctacg aagcacactt ctattaatat tgcaggagct
     2281 tttgtatgac taagccagta aagacctctt gcgtccgatt ttgcacctct atttttaaat
     2341 attattgata gttttttgtt gactctttca gcatatacct taccattgtt agttttgtat
     2401 attgtctctg tacccatagg atttagagtt gttttatttg cgttgaaatg gatttgtact
     2461 gctaagtcta cattttgcct attggcaatt tcacattgtt ctgctaaata gttattagat
     2521 ttatctactt ttccagtata cacaatagct ccaccttgtt tcaaccattt aacaattaaa
     2581 tcatttaaaa ttctgttttc ttttccttca tctatataac caactgcccc tgttcctttt
     2641 cctgttagtg tatgtcctgg tactattact actttcatca tttatcacct tctttctgta
     2701 gcatcacctt tatatctgtt acatcttcct ttatttcctc cacatcagtt ttcattgctc
     2761 ccatttcaac aagtatattt ttattgattt cctgttgctg tgtagatagt tcaataaagt
     2821 tttccactgt ctttttatac atgtctctat cttctttttt ctcttgcata gtatttttga
     2881 ataataaagc acataagatt cctattgctc ctaaactact taattctgtt aataattgtt
     2941 ccatgatgac ctcctactaa aattgatata aaaaaagaac cttttatact ttatataata
     3001 ggttctattg gtgtatcttc tttgtttaat aaatttgtta actctgtata ctcactttct
     3061 tttattctgt tcatagcgta aaatacatct aacttagtct gcaactcttc tttagtttca
     3121 taattttttt gttcaatcat tctttttaaa atattataca tgttgttccc tccgtttttt
     3181 atagttttat aacacgttat ttgtgttaag ttctaatata gcaattctat aagcattatc
     3241 tattaataga ttgtcttgct ccttttgatt ttcttctaaa attgtacctc tatcaagtga
     3301 ttgttctggt ttgactggat tgatttgtaa ttctaaccac tcattataag actgttcacc
     3361 tgttttaata atttctcctt tgcttattaa aggtgtccac aattgtccat tatctgcaat
     3421 aaaaaaatta tcaattccta tattatcaat ttcttctttt gaaaaattat gttctcttat
     3481 tattctaaac tttattttat tcatttttaa acctccaaat ttacaataac actaatcaaa
     3541 attcccttgt ctgtaatatc atgacctgga gttaacatca gtatagtatt atttacacaa
     3601 gctataccta cagtatcaac ttgttcacaa gaagaattca taactggaca agaatagttt
     3661 tgtcttgata ttcctaaagg taatttaatt attgcttctt ctaaaacagt agaagatgta
     3721 ctctttgtaa caatcatgtt tacaaataag tattttccta cctttgtaat agtagctgat
     3781 tgccatactt tatcccatcc atttatgaat ttaaagtcaa cattactttc agttaatgtt
     3841 gtaatgtctt ttaaattttc tctaataact tctatatggc tacctaaatt tcgtgtactt
     3901 tcaaaactaa catttggtat cgcaatacct gagctacaaa taaatcccgt ttgattatca
     3961 tattgctcta aatttagtag accaagttca acttctcttg gtatttctaa ttcataaatt
     4021 atttttaaag gatttttctt taatttttct ttaaaattag caatgctaaa aggagatatt
     4081 gaagtatcta ctatatctat tacacctatt ccattttctg ttgatataga caatgctgat
     4141 tcggtctgaa cattaaatat atagtctaca tgagattgct tgtataagtt agttataaac
     4201 ttagacatat ctaatcttaa tacagatata tctaaattta atctaaactc atacattcca
     4261 tgtacttcta tccagttttc attaccatta aaaactattt cttcacatct ttttattagt
     4321 ttataggaac catttttgta aactatttca tctcttattc catttggtaa acctcttaat
     4381 gtatgtggaa ttgtcttttt atcatattta tttcctttag ttataattgt tttattccca
     4441 ttatcatgta aatcttttat tatagtaact cttagatact ttgcattttt aggacttcta
     4501 gcaacataaa aaatatcttt atctcctatt attctttcat ttaatggtgc tggtatgaaa
     4561 cttttattta tatcatacca acaaatattt ctactacaat tataaaaagt atactctgta
     4621 tctggttcta tttctatata gtctagggta tatttatgat tactagcttc agaaataagt
     4681 tcctctccat taagatattg taaaatataa ttatctttaa aatcggcatt cttattaaag
     4741 atattaattc cactatattg ataacttaaa aattctattt tatctctttg cccaacactt
     4801 aatagttcat tagagtaatc tatattaata tctgtataat ctccttctaa tatggtaatt
     4861 ttcaaactta cttcataacc agatgtaagt gtatcgttat ctaaaacact ccttaaaaca
     4921 gcattacaat cacttaagtc tgatattgtt gtaaatgtat atttaaattt accaattact
     4981 cctcctggta tagctatatt tgtcagatta ggaataaata ctgaattttc ttcgcctaaa
     5041 ctatgaatat atattccact tgtactcggt aaagtgtttt tatcaacatc aactatgatt
     5101 gtatatatgg tatctggttt atatgaagtg taattgattg taaaaaaatt agaatatctt
     5161 atatcatttt ctgttagtat atttattttt ccatctacaa aagttgcttt ccataaagaa
     5221 aaatctgaac tagtttttcc ccataaatca attaatgtct ttccttctat attaaaatta
     5281 gttaaatagc cctttttgct attttctatt gtatattctc ctgtatcagt actacatttt
     5341 atattgtcaa tttctttgag attttctata tcattaattt tattttctag aacttctaat
     5401 aatgctttta tctttacaga agaataagta gttgtttcag tacttctatt atcatcaata
     5461 gttgctgaaa tcatgttaat agctgagttt ccatttacaa ctatacattc caattcattg
     5521 tttaattcat taaaaacttt tatactatta tcatctaaaa tttggtatga gcaaaatacg
     5581 ttgttgttag tagatttatc tattaaactt acaagcactt ttctagttac aaggttatgc
     5641 tcaactatag ctgtgtatac attttcttct aatatccagt tatttattga tatatcttta
     5701 gatacagata cattaggacc tccctgaatt aatttttcta aatcttcttt atttgctttt
     5761 ttactaagtt ctaggttaat attatcctgc tcattattta atttattaat attttttact
     5821 acatcttcat ttaacaatta atcaccttct tttgataaaa attatcttgc tgtataaaca
     5881 tcatatgtta tttcattttc atttgggaaa tatgttccat gtaattctct aactccacga
     5941 agagctacac cacttgttat actgactgtt gtttgtgtta ttgtagtgtt ataaatttga
     6001 atattagtac tacttgaata taatgttatt gtattagctc cagaaccttt atctcggaat
     6061 tgagttggaa ttattatttt atttccagta tataaagttc tatctaaagt tgtgccattt
     6121 ccattactgt ttgtactaat ttcagtagtc tttgtaaaat aggatgcatt attaagagaa
     6181 tttacaagtg tacttctata tgattgtatc tttgaatcaa tcttactaca tgccattgta
     6241 gattttatta ttgcacttat agcaacaggt tttcctgcga tggcagacat ggctgtcgag
     6301 cttcctgcga tggcagacat ggctgtcgag cttcctgcga tggcagacat ggctgtcgag
     6361 ctggagatta ctatgtttaa gaaccctgaa ttatttatta ttgtattcac gccaattt
//
LOCUS       DAELJO010000199         6414 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELJO010000199 DAELJO010000000
VERSION     DAELJO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.201"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            233..1147
                     /locus_tag="KPB88_003289"
     CDS             233..1147
                     /locus_tag="KPB88_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG0055528.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            1532..2179
                     /locus_tag="KPB88_003290"
     CDS             1532..2179
                     /locus_tag="KPB88_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0055529.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMSVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(2346..2915)
                     /locus_tag="KPB88_003291"
     CDS             complement(2346..2915)
                     /locus_tag="KPB88_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0055530.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(2920..3819)
                     /locus_tag="KPB88_003292"
     CDS             complement(2920..3819)
                     /locus_tag="KPB88_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0055531.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCRLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(3830..3979)
                     /locus_tag="KPB88_003293"
     CDS             complement(3830..3979)
                     /locus_tag="KPB88_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055532.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(3972..4556)
                     /locus_tag="KPB88_003294"
     CDS             complement(3972..4556)
                     /locus_tag="KPB88_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG0055533.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNGEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            4945..6270
                     /gene="cdeA"
                     /locus_tag="KPB88_003295"
     CDS             4945..6270
                     /gene="cdeA"
                     /locus_tag="KPB88_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG0055534.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
BASE COUNT         2301 a          935 c          788 g         2390 t
ORIGIN      
        1 aaaaatttct agtattactt gtactcttaa taaataaaag ccaacaagta ccttaaatca
       61 ttatatctat atctaataag aaacacagta agataatttt ttcgcccata acttaataaa
      121 aactgttgat attagtagca attgaattta gttaatttat ttataatata cataaattta
      181 aatattacga ataaagtatt ataaatagga tactttctaa agggaggaaa ttatgataga
      241 taatcaaaaa tatgttattt tatcactaga attacattta tttttttcaa gaattatgaa
      301 agagcatgct ctttttttag aagcaggatt cacaaataaa aattataatc ttgctatgga
      361 agctgaccac tataaaaagc aatttgaaga tttattatca tacactgtta gtgctagtaa
      421 tggtataatt agacctgata tattatattc agaagagctt gtaactactc tcacatcagt
      481 tgcagaacaa aaaacagaag agtttacagg gatagaaata aacaaaaaca tcactacaag
      541 agaattaaat ctacaaagtg gtgtaaaccc acaagttggt caagacttag tgaactatgt
      601 agctcaactt aactctgatg caataagatt acttgatggg cttattaatt ttaaagaaag
      661 agtcttagat ggtgtactat catgtactat atttacatca aactaccctc tactccttga
      721 acatataata catgaagcaa atttatatcg ttcttatgta gttgaccttg aaaataaaat
      781 agatattgag tcaaaaaacg ctaaagaaat agaattattc tgggaccata ttatgatgga
      841 acatgctctg tttatgagag gattactaga cccatcagaa ggtgaactaa taaatacttc
      901 aaatgatttt gctataaaat ttaatgaatt aattgaaaaa acaaacgaaa tgactgattc
      961 taatatcaag aacattacag aagaaactct aaatgaaact gttgagttta aagattttaa
     1021 agaagcagga gcatcaggaa tagaacagtg taagataaaa tctataatat taccactttt
     1081 agcagaccat gttttaagag aggcaaatca ttatattaga atattggaga gttataaaaa
     1141 catgtaatta aaaattactt ttaaaataat ctttgcatat ttagataaaa taaaggtaca
     1201 gttaactgta cctttatttt ttataaactt ataaaattta aacaagttat tcataaaata
     1261 ataatcatct ataacccaca actttatttt atattaaata aaaagtatta gtgattatgt
     1321 tttataagta tttctttatt cagttattta ttaaaaacta atagtaaatc ttatactaat
     1381 cgcatattta tgttataact atattgtaac tattttcgta atcttaggaa aagattactt
     1441 ataaaattaa ccttatacaa taatatatcg tataatctta attagcataa tagttacaat
     1501 attaaaattt aaatatggga gtgattttag tatgataaga caaacaccat tagagaaacg
     1561 atatgacaag ctaggtcctc atattgtaac agcactcaaa aaacgttatt ttgatgctta
     1621 ttactgtaaa actagaaatg aagctttgca acaaatttta gatttaattc ctcaaaatca
     1681 tgtagtatca tggggaggat ctgaaacact taaagaaatg agcgtacaga ctgctgttag
     1741 agagaaagga tttaaagtta ttgatagaga tttagcaaaa actccagaag aaagaactga
     1801 aattatgcgt caagcacttc tatgcgatac ttttttgatg agtagcaatg caattagtga
     1861 agatggacaa ctctttaata ttgatggaaa tggcaatcgt gtagctgcaa tgatatttgg
     1921 tccaaaaaat gttatagttg tagctggtat gaataaaatt gtaaaaaaca ttgatgatgc
     1981 aatacaaaga actagaacaa ttgcatctcc agcagttgtt cagcgttttc aagatgtcaa
     2041 tacaccatgc tatattaatg gaagttgtat agattgcaca agtcctgaaa gcatttgtgc
     2101 atacatggtt actacaagaa tcagtcgtcc agcaaaaaaa ataaaggtaa tattggtagg
     2161 agaaaatctt ggtctataaa atatacatac atgtaaatag ctctataaat aataaaatca
     2221 tatgaaataa cttatttcta aaagtggtag aataaaagct tctacaagac tttcattcta
     2281 ccacttttat attcttttaa tttgtgtttc tatattatta attgctccaa ttttcatata
     2341 tattactaac tatttttatt ttcataattt ttttgttctt catcaagctg tttataataa
     2401 tctttattat agtcagctag ataatctata cctcctcctg taatcacatt taataatttc
     2461 attgcagatt cttgattaac tttacaaaca taattatcac cagatttaac aagctctaat
     2521 gtaatattaa attctttttt ctttgcagaa tcaatatctt tcatagttgg atttggcatc
     2581 atttctccta gtttaggagg attctttttt aaaatagaaa ccatatcagt attgttaatt
     2641 tttaaatcta cagtcacttt atccttatta ttctttatat ctgaaatttc ccaagtcata
     2701 tttttagaat acattttaaa tccttcttta tcttcgtata taagattacc taaccctgat
     2761 tttgggaaat acttatcaac agtatctgca tcttgtgttt ttattgaatt aaacatacta
     2821 tccacttcat tttttatctt attatcatta gtacaaccag ctaacaccag tactgatatt
     2881 gcaaacatta taatacatat aataaatttc ttcattaaat tatatccccc tatacttttt
     2941 tgcacaaata ttattctctt ttttatcttt tgaaaaagtt gtagtaattg cattttgtgg
     3001 acaaactttt atacattcac cacacctaat acagtctgga ctatttacat ctttatatat
     3061 tggtatatca agcctacatt ttgtttgaca tgctgtacaa tttgtacatt tatctttatc
     3121 aattttaaat ctatatattg aaataggatt aaaaaaacca tagattgctc ctaaagggca
     3181 tatatacttg caaaatggcc tataagtaat gattgacatc aataatatca atagcaaaat
     3241 acttaacttc cagctaaata aaaatcctat tgattctctt aaattctcat ttcctaaaat
     3301 aagtggtatt cctcccaaaa gtgttcctga agggcaaatc catttacaaa accaaggatt
     3361 tccaccccct gctacattta caatagcaac tggcaaaata attacaaata ttattaagac
     3421 tatatacttt agatatttta gatatttatc accataaact ttttttatct ttactggaaa
     3481 gggtacttta tgtagtaaat cttgtacaag tccaaatgga caaagccaac cacaaacaaa
     3541 tctgccaaaa aaagcaccaa atgccattaa aaaaccaaca acataaaatg agaatttata
     3601 ctctctgctt gataaaactg cttgtaatga acctattgga catgctccaa gtgcacctgg
     3661 acatgaataa caattcaatc caggtacaca taatttttta ctttctcctc catatatagt
     3721 tccttctaaa aatccattta catatccatt tgttaatgct gtaaatgcaa cttgagtaat
     3781 aagtcttata ttattattta ctttagataa taacttcatt cctcttctcc taaccaatac
     3841 caatacactc aagacaaatc ataattgctt tagttaatac tgtatttgtt tctccacgat
     3901 atgtacctaa tgcaataaat ataatactaa ttgcaataat agaaatgcct tttttattat
     3961 ctttaataaa gttactcatt ggcttttacc atttttagtc tttcatctat aatttttttc
     4021 caatcttcct tactatttgc acctacaacc ttctcaccca caaagtttcc tttcttgtct
     4081 acaaaataag ttgttggaaa ctccataact cttttaaaat cagttttttt aagttcttca
     4141 gatacagtca attgttggta agttgcatct gactttttca taatgtcttt aactaaattt
     4201 ttctcttcat ttgaaagacc agttctcata tcagttttat caacttctac aactaatccc
     4261 tttatagcta cattggaatt attactctca tattcttttg ataatgcttc aagctctggc
     4321 atttcaccca cacatggtcc acaccaagta gtccagacat taataagagt aagttcttta
     4381 tttgaaaata tctcttttgt taccttattt ccatcaacat cctccgtatc taaattacta
     4441 aaccattcac cattattctt gtttttttct actggcttag attctttttt gctacatcca
     4501 gaaatcatac ctaaaacaca aatacatcct aaaactacta tgaaaaacct tttcatatct
     4561 cacactccta gaatttatat agtatataaa atcgctgctt gaaataaaaa taacttatct
     4621 tctatattaa agtaattata tttaataaat aagagctaat ttaaaaaaag atatacagtt
     4681 aaaatctatt tcttttcaga caaaatctta tatatatata atagcttgta aatcttaaga
     4741 ttatatacat atatttctaa agattttctt aagaaacagt tagattttac taatataagt
     4801 ttattaatta gcattatact aatttatctt aatatgattt tttgttgatt ttaagctgtt
     4861 tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat tatatataga
     4921 gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt cactactttt
     4981 gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc tttgtacaca
     5041 ataatagatg gcatctttgt atcgacatta gttggttctg atgctcttgc tagtataaat
     5101 attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc aactggtgga
     5161 ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa ctctacattt
     5221 tcttttatag ttttgttttc attgattgtt gggattttat tcacagtaat ctcatatttc
     5281 tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc atattgtata
     5341 acttatggta aagttatgat tttatgtacg ccattttata ttttaaaatt tatatttgag
     5401 tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt cattggtggt
     5461 gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg tcttttagga
     5521 gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat tatttacttc
     5581 ttatctaata aatctacact aaaattaaga aaaccaaaaa ccgattttag acttataaga
     5641 gatactatga taaacggttc ttctgaaatg gttacagaat tatctacagg aattacaaca
     5701 ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc tgctcttact
     5761 atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc tgctggagtg
     5821 tccccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa agaaacattt
     5881 aaacattctc taaaatttat atttgtttct tctcttttag tgtttattat tgctttagta
     5941 tttgcaccat ttattgttag ggtctttgta agtccagata acacagtatt taaactagcc
     6001 ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa tatatttgca
     6061 tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc ttttagtcgt
     6121 gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa catgactgga
     6181 ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat tctatttata
     6241 aaaaaatata aatgtagata taagtattaa aaattaatac ttaaaatcaa catttattaa
     6301 aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc aggttttatt
     6361 ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt aata
//
LOCUS       DAELJO010000200         6393 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELJO010000200 DAELJO010000000
VERSION     DAELJO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.202"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(268..867)
                     /locus_tag="KPB88_003296"
     CDS             complement(268..867)
                     /locus_tag="KPB88_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0055535.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(970..1767)
                     /locus_tag="KPB88_003297"
     CDS             complement(970..1767)
                     /locus_tag="KPB88_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0055536.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(1880..1986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2078..3064)
                     /locus_tag="KPB88_003298"
     CDS             complement(2078..3064)
                     /locus_tag="KPB88_003298"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0055537.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            3548..5278
                     /locus_tag="KPB88_003299"
     CDS             3548..5278
                     /locus_tag="KPB88_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG0055538.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            5388..5468
                     /locus_tag="KPB88_003300"
     tRNA            5388..5468
                     /locus_tag="KPB88_003300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5422..5424,aa:Leu,seq:tag)
     gene            5803..6318
                     /locus_tag="KPB88_003301"
     CDS             5803..6318
                     /locus_tag="KPB88_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG0055539.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         2206 a          890 c          953 g         2344 t
ORIGIN      
        1 aattaagtaa aattattttg tactgaataa atttttactt tatttacaga ataatgatta
       61 tttacagaaa agcgcgaaaa gtgtattcct ttaagtgtgg gatggatagc acaaataggg
      121 gagaaaaaat attggccata taagaatgca taataatata aatatttgaa aggtcagcat
      181 catataaaaa ttaaaaagaa aataaataat ttttctataa aaaagagact atgagaaaat
      241 ttactcatag tctcctaaat aatgtattta ttttcttcct gcaacccatt ttaatcccca
      301 atgaaattca gtatccattt ctctatgacc tttgaagtat ttaactactt tttcaacact
      361 gaatgtctca tcatttacat tttctatcaa tgctattgaa cacatattat agccattttt
      421 atgttcatca agtttgacaa ctaagtctga atcttgtttt tgtttgattg ttacaattcc
      481 atcagcttca gaccagttag ctacaccttc gtatataaaa gcataaataa gtattctttt
      541 tatatctttt attttattac catttattct taaattttct ccttgtgtat tacttcctgt
      601 tctgtcatct ccatctaatt gagcaaaagg tggattgtct aaactaccaa aggcatttcc
      661 taatgcttga acagcacctt ttacaccatt tttcatctca aataggcaac ccaaatctaa
      721 atctatatta ctgcttctta aagagccaaa aaaacccttt ttttgtactt tttgattcca
      781 atttaaatta actaagattt cacctaaatc gctattttcc ttttttgtaa ggtttatttt
      841 ttgtcccttt tttaattcta tagccatagt aatcttcctc cacaaacata gatttattta
      901 aatggtagca cttaataagt caatattcaa caagattaga ataataatat taaaattata
      961 ctaaatatac taaaaagtag gaatcacaat acctttgtat ttttcttcaa ttaagcttct
     1021 tgctttatct gatgtaagaa catcttgtag aactttaatc ttttcactgt ctttatcttc
     1081 agttctaaat gctaaaagat ttgcatactc gtcatttata ggagctgtat aaattccgtt
     1141 tttcttggca tcaagaccag caggtacagc ataagtagta tctataaatg cagcagtcac
     1201 atctggaaga gaagatggaa tttgagcaat atctaactct ttgaattgta ttccctttgg
     1261 attttctatt atgtccttag gactaactaa tccatcccct ttaggtaacg ttaaaagtcc
     1321 aatactttca aggtatctaa ggttcttaga acaagaagat gggttattgc ttattgcaat
     1381 tgtatctcct tttttaaatt catcaacaga ttttatcttg taagagtaaa gtattgctgg
     1441 gcatacatat agttttttac cagccattat tttgtaacct ttggccttaa cagcttcttt
     1501 tagataaggt tcatgttgaa atagatttgc atcaatttca ccattattta aagcttcgtt
     1561 tggaagtatg taatcatcaa agtttttaac ttctaaagta tatccttttt ctttaataag
     1621 tggttttaat tcttctaata actctcctcc tggaactaaa gtagcaccaa caacaatttt
     1681 tttatcatct ttagctttag aacatccaac cacagctaaa gttaatacta gacataataa
     1741 caaacataat aattttttga atttcatata aattccctcc tgaatataat tgattagtta
     1801 aaaataaaat accattcttt atcacactgc aaaataaaaa taagcctgag tttaaactca
     1861 ggctttaaaa gctaatttag actcatcttt cagtataata ctgcaggatt tagcaccaat
     1921 gtatatagaa aatatactgg ttgctgggtt tcatagggcc agtcccttca ccactctgga
     1981 taagaagtat caatttttaa ttatgttata aatattatca tgatttttta cgtctgtaaa
     2041 cataatttta taaatatttt aaaatcttta atgtatttta tttaacacct aaaattccag
     2101 aaactagaat atcagtatca gcacctaaga aatagtcatt tatttcaata gaatccagag
     2161 tatttctcaa agcattttct tcatgcctta taccaataag tttattttct acaaatgcaa
     2221 catcacattt tccaaaaaaa tctccaaaaa attttatact agttattatg cctttatcaa
     2281 catttaaatt aaactcaaca tttccaccag aatattttaa ttcatttgaa agagtatatt
     2341 ttggtgaatt accaaaattc caatcccaag tttcgtattt tgatttagta agcttttcta
     2401 tttgttttat atcatgctca ctcaaacaat aattagtatt atcagtattt gttttagaaa
     2461 gataatccat tatagcatct ttaaattcta atatgtccat ttttgaatgt aaatgttcac
     2521 ttatattggt tacacgagat tttacagatt ttattccttt tccttcaaat tttataggtt
     2581 taacctttaa tgcttttgag aggtcattta tttcagatga aaataaaaga gtcccatgat
     2641 gcattacttt gtttttgtag ttgtattgag catttccaga aaatttcttg ccatctataa
     2701 gaaggtcatt tcttccagaa aattcggcat tgacatctag tgttttaagt aaatctatta
     2761 tgggctgagt gaatcttctg aaatcactaa aattattatt gttacaagct ataaatgtaa
     2821 aatttatatt tcccaaatca tgaaatacag ctccaccacc agactgtctc ctaacgacag
     2881 gtatagaata ttcttttata tattctaaat ttatttcaga aagtgtgttt tggttttttc
     2941 caacaattat agaaggttca ttcctccata aaataaataa gtcttcatca aaatttttta
     3001 gaagatattc ttccattgct aaattaaaat aagggtttgt tttttcatta taaattaata
     3061 acattaaagt agttcctcca tagaattgat aaaattttta ttatgaatac aattaatttt
     3121 ataattcgac aataatataa aaactccttt aaattagaaa ttttaaaaac taccttgcaa
     3181 agtttaaata aataatatat actcaaaata tgtttaataa aagtgaatag aagggtagag
     3241 gcgcaataat ctatcagtac taataaggag ttaagcacag agatttatta ggaaaggaga
     3301 tattgccgaa gttttataat cgatgcttta agattatatt gctgggggta catagaaaat
     3361 atgtacaact gtcacaggtt tatttgtggt gagctatcat ttaaggaatt atattatatt
     3421 tatttttact attgttgata tattgtaatg tttaagcctt gagtgtttac cactcaaggc
     3481 ttttttagtt ttattgtaac ataataccct gtacagttca caaaattaaa aggagaggga
     3541 gatttatatg aggaacaaaa aaattaatat tatttttctg acgacaatca tgttcataat
     3601 gtcaacagta atggtttttg cagaagaaga tatagacact attgcactag ccaatgcaga
     3661 aaaatttggg attttaactt taataccacc attagttgct ataatacttg catttataac
     3721 taaaaacgta ataatttctt tacttatagg tattctttct ggtagtttta taattaaagc
     3781 tagtggtatt aatgtttttg cgacatttat acaggcattt ttagatttag tagacagagc
     3841 tttagtatct ttagcagacc cttggaatgc agggataata ctacaggttt tggcaattgg
     3901 aggagtaata aatcttgttg ctaaaatggg aggagctaaa gctatagctg aagcacttgc
     3961 taaaagagca aagtcagcaa aagggacaca gcttataaca tggtttttgg ggcttttagt
     4021 attctttgat gattatgcca attcactaat agtaggtcct atgatgagac ctgttgcaga
     4081 taaaatgaaa atatcgagag aaaagctagc atttataata gatgccacag cagcaccagt
     4141 tgctggttta gctataatat ctacatggat aggtcttgag gttggtttaa tacatgatgc
     4201 ttttgaaagt ataagtatag atgtagatgc atttggtata tttttaaata caataccatt
     4261 tagattttac aatatattaa tattagcttt cattgtaata tcagcattat tattaaaaga
     4321 atttggtcca atgagaaaag ctgagataaa atctagaagt agaaaaatta gtatagacct
     4381 tgatgaaggc gtagaagagt tggatgattt agctccaaaa aatggagtaa aattgagtgt
     4441 atggaatgct ataatcccaa taggaacatt gataatagtt gcattagcat cattttatta
     4501 tagtggatat acatctataa tgggaggaga cgataaggct ctgatacaac tatttacaaa
     4561 ttctccatat tcttttgaag caataaaaga agcatttagt gcatctgatg catctagagc
     4621 attattccag tctgcacttg ttgcaagttt agttgctatt ataatggctg tagtaaaaaa
     4681 gatttttaca atatcagaag caattgatgt ttggatagat ggaatgaaaa gtttagtaat
     4741 cactggtgta atattaatac ttgcatggtc attaagttca gtaataaaag agttaggtac
     4801 agctaaattt ttaatacacc tattatcagg ttcactacca ccattcttat taccaagttt
     4861 aatatttggt ttaggagcaa taatatcttt tgctactgga acagcttatg gaacaatggg
     4921 tatacttatg ccacttgcaa ttccattagc atactcatta aatcctgata tgtcttatgt
     4981 aatagtaagt actagtgcag ttttaacagg tgcaatattt ggagaccatt gttctccaat
     5041 ttcagataca acaatacttt cttcaatggg agctggctgt aatcatatag accatgttaa
     5101 cactcaaatg ccatatgcta tatttacagc agtaatcact attgtatttg ggtatatacc
     5161 agctggactt ggattgccaa tatatatagt attaccagtg gcaatagctg ctatatttgt
     5221 aggtattcaa ataattggta agaaagttga tgaagcggaa atagaacttg tagaataaag
     5281 atataaataa gtttcacctt ttattatcac tattttgtta gtttttagat aattttaaaa
     5341 agttattgaa aataatggcg aaatatagta aaataaaaga gtgataagcg gatgtggcgg
     5401 aattggcaga cgcactagac ttaggatcta gcgccattgg cgtgggggtt cgactccctt
     5461 catccgcact ttacagtact taattgtact gaaaaaaact ggggttgctg gctttcggag
     5521 tgggctatcc cgtttttatt tttttgtata tataatagat tggcacttta gtataaggtg
     5581 ctttttttat aggcaaaaac agtacattta tttctgtttt attatcattc atctattaga
     5641 atatacttta gttaagaatg tgttaataag cagtgttcac attcttgata aaatgtggaa
     5701 taatatgtat ttttactaag acggaaatga aataattaaa ccattaaata atattataag
     5761 atgatgaaca agtagataaa tcaaattttg taggaggata ttatggaaaa atatatatgg
     5821 ttgtttccat tactttttat ttttcatgat atggaagaga taataggttt tggaatatgg
     5881 cttaaaaaaa ataaatcaat gcttgataag aagtatccat ttatcagtaa aatttatgaa
     5941 aattatagta ctgaaggcat ggcatttgct gtgtttgaag aatttatttt atgcattatt
     6001 ttctgtatac ttacagttat aacagaaaat cagtatgtat atctactatg gttaggtagt
     6061 tttatagcat ataccttaca tttggtaatt catatagggc agtcaatcat aataagaaaa
     6121 tatatacctt cacttattac cagtatcata tgcttaccaa taagcatttg gtgtataagt
     6181 aagagtattt atatagttga ttgtgaaatg agtacaacaa ttctgtacag tataattggc
     6241 attataattg tagcattgaa tttaaagttt gcacagtcgc tgattggaaa atttacaaag
     6301 tggatgtcaa acatataaat tttagtttat tggacagttc aaggaatcag aaattggaat
     6361 tttatagtat taatatttta tagtattaat ata
//
LOCUS       DAELJO010000201         6389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELJO010000201 DAELJO010000000
VERSION     DAELJO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.203"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            235..1599
                     /locus_tag="KPB88_003302"
     CDS             235..1599
                     /locus_tag="KPB88_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG0055540.1"
                     /translation="MDIKELVKMQRKYYNTGKTRNISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVSQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1738..2904
                     /locus_tag="KPB88_003303"
     CDS             1738..2904
                     /locus_tag="KPB88_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0055541.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            3021..3803
                     /locus_tag="KPB88_003304"
     CDS             3021..3803
                     /locus_tag="KPB88_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG0055542.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3922..4575)
                     /locus_tag="KPB88_003305"
     CDS             complement(3922..4575)
                     /locus_tag="KPB88_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055543.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4599..5963)
                     /locus_tag="KPB88_003306"
     CDS             complement(4599..5963)
                     /locus_tag="KPB88_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0055544.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2436 a          776 c          948 g         2229 t
ORIGIN      
        1 attattaaag atggataatt taattgtttc aataaggtta taattttaaa aaatgtttat
       61 tataaaataa attattttat aagcttactt atacttaata aggggctttt tttattggaa
      121 gatttatgtt atattgagat gaatttatta tgatataaaa aataatgtta ggataaattt
      181 aaatcttttt ctaaaaatgt attaatataa ttcttaaaga aaggattttt aataatggat
      241 ataaaagagt tggttaaaat gcaaagaaaa tattataata caggtaaaac aagaaatata
      301 tcgtttcgta tagaacagtt gaagaaactt aagttagttg tatcacaaaa tgaagaaaaa
      361 atacttttag cactaaagaa agatttaaat aaatcggatt ttgaaggttt tatgacagaa
      421 gtaggcatgt tttatagtga attaaacttt gctataaaga atataagaaa atggagcaaa
      481 ataaaaagag ttaaatcaag tatggttaat tttccatcta tatccaaagt tgtttcacaa
      541 ccttatggtg ttactctcat aatgtcacct tggaattatc catttcaatt agcacttata
      601 ccattagttt ggtcattagc agcagggaat tgtgttatat taaaaccttc tgaatattct
      661 gtgtcaacat ctagtgtagt taaagacata gttgaagata ctttttcaaa agaatatgtt
      721 gcagttgttc aaggaagtca agaagaaagt gaaaagttac tattggaaag atttgattat
      781 atatttttta caggaagtac taatgttggt aaaataatca tgaaatcagc atcagagcat
      841 ttaactccta ttactttaga acttggagga aaaagtcctt gtatcatttt aaaagatgct
      901 aatatagacc ttactgccaa aagacttact tggggaaaac taataaatgc aggacaaacc
      961 tgcgtggctc cagattatgt tttagtgcat gaagatagaa aaaatgagtt aatagagaaa
     1021 ataaaatatt atactaataa atattttgga gataatccat gtaataatga gcaatttcct
     1081 aaaatcataa atcagagaca ttttaatcga atattatctc ttattgataa agataagata
     1141 gtctatggtg gaaattataa taaggagaca ttaaagatag agccaactat agtagacaat
     1201 gttaattggg atgataatat aatgaaggaa gaaatatttg ggcctatatt tcccatttta
     1261 acatataaag atttggatga agtaatacaa aaaattatac aaatgccaaa tccattggca
     1321 ctttatattt ttacaaaaaa taagtaccta gaaaataaac ttttagaaat gattccagca
     1381 ggaggatgtt gtatcaatga tacagttact catatagcta cgaattactt accttttggt
     1441 ggtattggtg aaagtggtat gggctcatat catggaaaag ctggattcga tacatttact
     1501 cactataaga gtgtgttaaa aaaattaaat ttggatgtcc ctattagata tgctccatac
     1561 gataataaat taattaaggt tcttaaaaag ataatgtagg ttatattgta gaataataaa
     1621 tataaataaa attgagtatt tgtattctga atatatttta tttttaattt ggtatatttg
     1681 ttataaaagt ggtgattttg tgataaaatt taattatata gaaataagga ggaaaaaatg
     1741 aaaattaatt ggaatacaaa atacacaaca atagcaattt atacatttat aatagctgct
     1801 agtagtatta tattttatct agtatcatca caaattgatg tattttctaa taatttagat
     1861 gctatattta caacattaca accttttata ataggatttg ctattgcata tcttcttaat
     1921 tttattttaa agttttatga agacagaatt tttataaaaa gtgagaaatt aaaaaaacta
     1981 aaacaaagtt caaaaagagg cttgggtctt ttattaacat atgcaacagc tacattgata
     2041 ctttacttat ttatgcattt cgtattgcca caagtaattg agagtattgt tggactagca
     2101 aatgatatac ctatgtatgt aaacaatgca actaagctta ttgataaatt aatgactgat
     2161 ttaaatcttg atgagcaata ctttaattta gcagttgata agtggaatga gtttgttaca
     2221 tatattataa agtttgtaac agatttaatc ccaatattag gaagtatgtt aaagaatgta
     2281 gcctctagta tatggaatat tgtactagga ttaattgttt cagtatactt attaattgat
     2341 aaagaaaaat tctatggatt aagcaaaaaa ataacttatg cagtatttac agaaaaacaa
     2401 gctgctagaa ttttagaatt aactcataga agtaactata catttggaag atttttaggt
     2461 ggcaaaatat tagattcatt cattataggt attctaacat ttgtaatact tacattagtt
     2521 aaaatgccat atacattatt aatatcagtt ataattggta ttacaaatat tatccctttc
     2581 tttggacctt tatttggagc tataccatcc acgctaatag tgatgtttgt atctcctata
     2641 aaagctttct ggttattgtt aattatatta ataatacaac agatagatgg aaatattata
     2701 ggacctaaaa tacttggaga ttcaattggt atatctgcat tttggatttt gttctcctta
     2761 ttggttgcag gaaaattatt aggttttatt ggaatggtta taggagttcc aatgtttgca
     2821 gtaatttatt ctataattaa ggatattgtt gaaagtaaat tggacaaaaa gggattaccg
     2881 acagatactt ccgattatat gtagctaaaa aatattatgt ataattaaaa atatataaat
     2941 taagcaatat ttaaagcact gagaaagtta aactcttggt gctttttaga tataaaatta
     3001 ttatagaaga ggtgtttttt gtggcattta tagaatgtaa atttaaatct aaatatactg
     3061 gaggagaaac tgatataatt gtaattttgc ctgtagcaaa tggaaaagaa ctttttagtg
     3121 gtaaagagat atatgatttt gaaggtaaat ttaaaacatt atacttattt catggtttag
     3181 aagatgacca aagctcatgg attagatata ctaatgtaga gcattatgct aaaaataaaa
     3241 atatagccct agtaatacca agagtagaaa ctagttttta tactgatatg gcgtatggac
     3301 accaatattt tacttttgta agcgaggaat tacctaaatt tgttagagct atatttcctt
     3361 tatcagataa aagggaagat aactttgtag ctggtatgtc tatgggggga tatggagctt
     3421 taaaattagc gctttcaaaa ccaaaagagt tttcggcagt tgcaagtttt tctggttcgc
     3481 ctgatatgat tcatgagtta gaaaatcaac aaatagaaac caatgcagac attctatttc
     3541 acgattttgg tagaccagaa gacgctagaa atagtgagaa tgatttactt tacatactta
     3601 agaaccttaa agaaagaaat gaagatattc ctaaaattta tcaattttgt ggtgatagag
     3661 attttttata taaaaataat cagatgttta aaaaatatgc agattctctg ggtgtagatg
     3721 ttgaatatga agagtgtgta gggggacatg aatggcgtct ttggaattta tggataaaga
     3781 aatttataga tataattggg tagtacactt atggtattaa caggtatact ataaaatatc
     3841 tttaaatatg ataaagactg aaatcctaaa atcaaattat gattttaaaa tttcagtctc
     3901 taagaattta ataaaatttt attaagttct tacacgagtt attattggag taaaattgtc
     3961 aactatatct atatagtatt tactcaattc ctctaaaatc tcttcgcata aatccgtact
     4021 atgaccaaga ttaataagaa actgttttgt aaaatgcttt attacagctt gagaagatat
     4081 atcgtacttg tgtgaagtat aaactctaat tatttcaggt ttcatagcaa gtataccaat
     4141 acaagaaagt attaattcat catgttctat agattcttta tcattaacat atcttttcat
     4201 gaccatttcg gttacacttc tttgatagct taaaatagct tcttcataag ctatatccaa
     4261 ataaaaattt ctatatgctt cgttttttag aaggaaacaa aggtcagcat tcatagcaag
     4321 aaagaacatc atcatagtac cttcttcatg gtagttatag attaaagact gcatttcatt
     4381 tcttatatct tcatatatta tagctcctat ttcgctttta cttgagaagt aatagcttat
     4441 tagacctaaa tttatattag ctctttctgc aatttgccta gttgttgtct tatggtaacc
     4501 ttgctcaaaa aataattctc tagcagcact tataatacta gcttttgttt ctttgccctt
     4561 ttctgatttt gacatattta ctgtgtatac ctctcttttc aatctttttt tgaaaatttg
     4621 ctaactataa atattgtaat agaaccacct attataggaa ccaaaattct gtcaaataca
     4681 gtaagaatac caatcatatt taataatgcc aatataacac ttactataac agcatagata
     4741 gctccttttt ttgtagcacc tttccagcaa agaccaatta caaatggtgc aaatattaca
     4801 cctgtacata atggagctgg ccaaaacaat aattctaata aactatcaaa tttaagtgct
     4861 atgattgcac ctattatagc tgtcaaagct gatgcaattt gagaatactt tagagtattt
     4921 tttaaatcat taaattctat attaggatgt aataatttgt tataaatatc tcttgatata
     4981 atagcactca ttgaggtcat taatgaatcg gcagtagaca ttatggcagc caatatagca
     5041 gctaagaata atacagatat taaaggtggc aaagtagtcg atatcatttg aggtaaaacc
     5101 tgaacagatg atatatttgg aaataaacta ccaccaatag ctccaattat tcctataggg
     5161 aaagcaagaa ataatagtat aaatcctgca attacactac ttttaactgc atttttagca
     5221 ttatcagcac attgatatct taaaaaactt tcataagaaa atattgagcc taaaactaat
     5281 ggtaaaactg tatacataaa atcactaatc gatttatttc ctggaaagaa tggttctttt
     5341 accattgatt gtaacataag taaagggttg tcgccaacta attttaagct tatataagct
     5401 actattggca aaaacactat acaaatagca agttgaataa atgctgatac tgcaactccc
     5461 cacattcctg atgtataagt atatataatg ataactataa ctcctataat tacagctata
     5521 tcataaggaa taccaaatgc agtagataca gaaccaaaag aaatcaattg actacccata
     5581 atagctagta tagatattgg tgttacaaga ctttgataag ctcttaaaaa tacactatga
     5641 ccatatctag tttcaatata atcagcaggt gttatatcac aagataagct tcttaatttt
     5701 cctgcgaaaa atgcatttat aaagcaagct aaagctgcac ctatagtaga atacattcca
     5761 acccaaagtc ctctttcagc tccccattga actccaccaa ctaagcaccc tcctccaaaa
     5821 gatgtagcag caatggttgc agctagtata ggcaaaggca gatttctacc tgcgattagg
     5881 aaatcggatg ctgttttaac tttttttgaa aaataaaaac caatccatat gtatattgca
     5941 cacgtaacaa ttaaaagtaa catagtcttt tctcctttat aaaatatttt aataaaatat
     6001 ataaaatata tttaaaatat attttatata aaagtagtac gatatttgtt ttattggttt
     6061 tagattaata tagctataat aatttatgat attagatgga aattacgtaa aaatagaaaa
     6121 ttgaaaattc tgaaattttt gcatattgat atttatctca tagattcttt tttgtaattg
     6181 taacacgctt ttatttttaa atcaatagta catgtataaa aataaaagga atttaaataa
     6241 gaaaaagata tttttaatat ctggatgtag tatagaaata tcttgaataa attattcttg
     6301 aataaaatta taaaaaatta aaatcactag aaatatgata aaataattta aaatacgcat
     6361 ttcaatgaca tttcaaaaca agaaaataa
//
LOCUS       DAELJO010000202         6238 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELJO010000202 DAELJO010000000
VERSION     DAELJO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.204"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            14..1897
                     /locus_tag="KPB88_003307"
     CDS             14..1897
                     /locus_tag="KPB88_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG0055545.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2121..2801
                     /gene="pgmB"
                     /locus_tag="KPB88_003308"
     CDS             2121..2801
                     /gene="pgmB"
                     /locus_tag="KPB88_003308"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0055546.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2798..5083
                     /locus_tag="KPB88_003309"
     CDS             2798..5083
                     /locus_tag="KPB88_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0055547.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLYFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            5309..6202
                     /locus_tag="KPB88_003310"
     CDS             5309..6202
                     /locus_tag="KPB88_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0055548.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         2603 a          561 c         1139 g         1935 t
ORIGIN      
        1 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgataca gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1921 tttcatgtta aattattata cagtttaaac aagtctttat aaacgataaa aaatatggtc
     1981 tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag tagataattt
     2041 tataaataat atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct
     2101 tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta
     2161 cagatactgc atattatcac tatatggctt ggagaaaact agctcataaa gttgggatag
     2221 atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca
     2281 ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg
     2341 ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca aatgatattt
     2401 tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat
     2461 cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg
     2521 attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta
     2581 tgtcagcgaa agggcttaat gtaaatccac aaaattgcat agggatagag gatgcaagtg
     2641 caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa
     2701 acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac
     2761 aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt
     2821 agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc
     2881 cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag
     2941 tggagataca cacaaggggt cttatatagc tggagtatgg tttccagaca aaactcgtgt
     3001 tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaacta
     3061 tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga
     3121 agatttttat agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa
     3181 tataaatgat aaaaaatttg aagttagggt tgctaggttt ttaagtatat ctgttaagga
     3241 acttgcagta attaaataca gtataaaatc attaaattct aatactaaaa taaaatttgt
     3301 accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa
     3361 tggaatttct tcaagttctt tagaatataa agggagtatt ctttctcgaa caaaggagaa
     3421 tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga
     3481 tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga
     3541 tgttttaaaa aatgaggttg caagtataga aaaatatgta tctataactt caactagaga
     3601 ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatagag aactatctaa
     3661 aagttatgat gatatattaa atgaacatac aaagttatgg aataaaagat gggaaagtgc
     3721 agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca
     3781 actattttca acatattatg gagaagattc gcgattaaat ataggtccaa aaggatttac
     3841 tggtgaaaag tatggaggag ctacttattg ggatacagaa gcgttttgtg ttccagtata
     3901 tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt
     3961 agaacaggca aaggataatg caaaaaaact agggcttaaa ggtgctttat atccaatggt
     4021 aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg
     4081 aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg actataaata
     4141 tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt
     4201 tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta
     4261 tgaaaacaat atcaataata attggtatac taattatata gctaaatggt gtttagatta
     4321 tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa
     4381 tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt atctgccgta
     4441 tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga
     4501 agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat
     4561 tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa
     4621 tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtcca
     4681 tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga
     4741 aaaggcttat gaattatatt caagaacagc taggcttgat ttagataatt acaataatga
     4801 tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg
     4861 atttgcaggc atgagaacat ggaatgaaac tctatatttt gaccctaagt tgccaaaaga
     4921 gtggaaaaat tattctttca atataaatta tagagataat attattaatg tagatgtatg
     4981 tagtgattta attaaaatag aaaatttaaa aggtaaacca ataaaaatta ttgtatatgg
     5041 gaaaaaatat atattagaaa aatgtataaa aattgataaa tagatttaat aatattttta
     5101 ttatataaat agttaaaata agcttatcta attatttatt tattttaact gttttgtgtg
     5161 atagaaaact atatattata acaatagtag tgcttttaat taaatatatt tcataaatct
     5221 tcataaaatc ttcaaaattt cgtaaagaat atgtcacaaa agtaaggtat attatattca
     5281 gcagatagta taagggagga caatatttat gaagaataaa aaattcattg ttataatact
     5341 gataatatca attttaatag gtgtattagt gaaggaatat tcatctagag agataaaaaa
     5401 ggatgatata aatgtcagta agtatataaa atatgctgat ttagcaagta aaaataatgc
     5461 acaagttaac tggaagtatg tagcttccat tgttgctgtg ttaaataaaa ataacttaaa
     5521 aaacgttaag gattcacaga tacaagaagt atcagattta tttgttaaga atttttctaa
     5581 aaataataaa ataaataagt taagtgatat tttggatgag ttagaattta gtaatagaca
     5641 aaaaagattg gttgataatt atatagacaa tcttaaggat tatggtataa aaccagaaag
     5701 attgaagtct gatacaaaat atatgaagtt tattgatgaa attaaaacag aagcaataca
     5761 aaattataag gattataaaa tacttccatc tataactatt gcacaagcta taatagaatc
     5821 tagctggggc aagtcaactt tagcaaaaca atataacaat ctatttggta taaaggctga
     5881 cgcttattgg aaaggtaagt cagtgacttt agaaacgaaa gaacatttag atacaattat
     5941 agatgataaa tttagaatat atgatgataa aaatgaatct ataaaagacc atgcaaaatt
     6001 tttagccaca aataaaaggt ataaaaacaa tggtgtattt gatgcaaaaa cgtatatata
     6061 tcaagctaaa gcattagaaa aggcaggtta tagtactgcg aaggatgaaa atggcaatag
     6121 tatatatgca gctcgtctaa tagaattaat tcaacagtat aatcttcaat taatagacag
     6181 tgaaattcaa agtgaaatgt aactttgatt aaatttttat tgataaggct atgatttt
//
LOCUS       DAELJO010000203         6234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELJO010000203 DAELJO010000000
VERSION     DAELJO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.205"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(35..1099)
                     /locus_tag="KPB88_003311"
     CDS             complement(35..1099)
                     /locus_tag="KPB88_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG0055549.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1361..2032
                     /locus_tag="KPB88_003312"
     CDS             1361..2032
                     /locus_tag="KPB88_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055550.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            2034..2942
                     /locus_tag="KPB88_003313"
     CDS             2034..2942
                     /locus_tag="KPB88_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055551.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            3019..3705
                     /locus_tag="KPB88_003314"
     CDS             3019..3705
                     /locus_tag="KPB88_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055552.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            3702..>6234
                     /locus_tag="KPB88_003315"
     CDS             3702..>6234
                     /locus_tag="KPB88_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0055553.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNSKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKNVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDI"
BASE COUNT         2462 a          734 c          954 g         2084 t
ORIGIN      
        1 ataaattcta gtacagattc tttattaaat aaaattattt caaccaagat ggatttccag
       61 gtataaatat tcttttacca aattttgaag tatactcatc taaagctttt tgaataagct
      121 cattattacc cttaaatgaa gtagtattct ttatagtttc gagattttgg ctaaatgggc
      181 aagcagatat acacatacca caatctgtac ctaaatatct ccatttaata tagcatgtct
      241 catgttcaat tacccaatta tattttccat catcaccaat tttaatatca ttagaaagcg
      301 aatgagaagg gcagttaaaa gagcattttt tacagacctt acaaaaatct tctagtccaa
      361 aatcaacata ttcatcttct aaaagaggaa tatctgtagt aacaacacct aatctaagtc
      421 tagagccata atctttattc gtaagtatag catttcttcc tatatctcct aaaccagcat
      481 cacgagctat aaaaacaggc attactaaat aattagcatc catatgattt ctagcatcat
      541 atcctaaatt tcttatatag taacttaaaa tcatgccgac gatagatgca tccacatagc
      601 actttgatgt ttctataact tcacatatca ttggagctct atttatcatt tctttatcca
      661 tctcacatcc aaatacaatt gcatacttat gagttagatt aatttcttca ccgtaatttt
      721 cttcatgtct acccctattt gtataaatat gatagtcttt taatttagtt ataccaacta
      781 cagaagctcc atactgcttt gcaagaccct taattttttt tgtcattgac tttgcatcta
      841 catctacttt agtatcagaa acttttcctt cgcataaaga tttgatatca gataaaaaat
      901 cgaatgcact acttgccatt ggagagttta actcattgta agtcattgtt ccttcactac
      961 ataaatttgg tctatttctt atactatcat caatttcttt tttgtcagga tttttcttat
     1021 aatagtcatt ataagcagta ctatcttttt tgtaatttgc tcttgcaaac atagtatttc
     1081 tctcatcaac ccttttcaat tctattcccc ctagtaaaaa tatatttaaa aaatataact
     1141 ttattgttgt ataatttaat tatataagaa taaagttata ttttcaattt aagcgtaatt
     1201 aaattgggaa atatataact atatgcttga ttatgatgtt tttattttga aaacaaattt
     1261 aaatacaatc tttttagtga aataaataaa attaaaatat ttttagtatg ataatgcaag
     1321 aaaataatta tttatctaaa aaatctaaag gagagtaaaa ttgaacttat taattattga
     1381 agatgatata aacttaaatg aaggtctttt ttatgcattt gagaatgatg gatttaatgt
     1441 atttaaggct tatacaaaac aagaaggatt aaatatattt aatagtaaaa atatagattt
     1501 tataatctta gactgcaatc ttccagatgg agatggattt gatgtatgtc aaataataag
     1561 ggaaaagtca gatataccaa ttataatgct tactgcaaga gactcagaaa ttgatgaagt
     1621 aaaaggatta gaaatagggc ttgatgatta tattacaaaa ccattttctt tatctgtatt
     1681 aaaggcaaga gtcaaagtag caataagaaa aaaatcgaat aataaagtaa tatattccaa
     1741 tggaataaaa ttggaccaaa agttactaaa agtatataaa aataaagaat gtttggaact
     1801 tagctctgta gaatataagc ttctaagtta tctaattgaa aataaaggtc aaatattatt
     1861 aaaggaacaa attttacacc atatttggga cagcgaagaa aattatgttg atgataatat
     1921 tgtatctgta aatataagaa gacttagagt aaaagtagag gatgacccat ctaatccaaa
     1981 atatataaaa acagcttatg gaatgggtta tttatggaat gaggtagaat aatatgttta
     2041 tattagcctt tataatatgt acagttgtta tatttatagt atctactaaa ataaaccaaa
     2101 gaagatatga tgaattgaat ttgatgttaa atcaaatatt ggaaggtaaa gaagttactt
     2161 atccagatac gaaggataca agagcgtcaa aaatttctca ccaagtaaaa aaaattaagg
     2221 atatgataga aatagaagta gaacagtcaa agtcagaaaa agaagcgatt aaaggtttaa
     2281 tatcaaatat gtcacatcaa ttaaaaactc cactttcaaa tataaccatt tattgtgaac
     2341 ttctggagaa tataaatatt tccacattac agaagaagga gtttttacaa aagatgaaaa
     2401 atgaaacttt taagattgac tggctgttac aatccttatt taaaatgact aaattagagg
     2461 atggagttat agaatttgaa gtagaagaac tactaattaa agatactctt attcaaagta
     2521 tcagtactat ctttaataaa gcagaagcta aaaatataag gataaatctc gaaccatttt
     2581 cagacataaa attagttcat aataaaaagt ggaccataga agcaattgta aatgttttag
     2641 aaaatgcaat aaaatactca ccatcagata gcacgattac tatttctgta ataaaaatgg
     2701 aactatatac gaagattact attaaagatg agggaatagg tattgatagt agagaactaa
     2761 atgatatttt taaaagattt tacagaagta aaaatgtgga aaaccaaaat ggcacaggga
     2821 ttggtctata tcttacaaga ttaattttag aaaaagaaaa tggaaacatt atagtagaat
     2881 ctaaactagg aagtggatgt tgttttagta tcttcttaca gaattgtaag agtttaaact
     2941 aattttgtag gatgagtgta attatcgtca cttacaatat tttatagaac atatatctgt
     3001 attgaaagga gaaattaaat gagtatatta gtgacgaata atttagtaaa acactatgga
     3061 aaaggtgaga ctaaagtaaa agccttaaat ggagtaagta tagaaattga acgagataca
     3121 tttactgcta tagttggaac ttctggttct ggaaaaagca cattgttaaa tattataggt
     3181 ggattagata atccaacttc aggtgatgta ataattaaag gaaagaacat atctaaaata
     3241 ggaaaaaaag atttgacagt atttagaaga agaaatatag gatttatatt tcaaaactac
     3301 agtttaatgc ccgtattaaa cacttatgat aatatagctc ttcctgttac attagataaa
     3361 ggaaaccata tagaccatga atatattgaa atgctgatga agacattggg aatttgggat
     3421 aagcgtttaa aatttccaag cgaactatct ggtggtcaac aacagagggt agcaattgct
     3481 agagctttgg caaataaacc tgcgcttata ttagcagatg aaccaacagg aaatctcgat
     3541 agtaaaacta caatggaagt agtatgttta ttaaaagaaa gtagtgcaaa attccatcaa
     3601 acgatactta tggtaacaca caatgaaaat atagcacaag tatgcgactc aattatccat
     3661 attgaagatg gagttgtggt gaatactggt ggtgaagtac tatgatgtct ctagtaaaat
     3721 tttcaattca atatataaag cattataaaa aacaatctat atctatagtt ttaagtatag
     3781 tactatcagt agcattattg acgggaattg gttctttagt tcatagtgca gacaaaagta
     3841 gaattgaaaa gataagggaa gatagtggag attatcatta tttctataaa gtagataagg
     3901 aacaacttaa taaaattaaa aaaaataaaa aatcaaagga atacactgta gatagacttg
     3961 gcgttacaaa acctaaaaat cagatagatg aaccttttat tatagaattt ttaaatgctg
     4021 attcttcata tttagacatg acaggtagaa aattattaaa aggaactctt cctagtaaag
     4081 atggagagat agtacttgac acatattctc taaacaattt aaatgtaaac aaagaaattg
     4141 gaacagaaat caatttagat ggtaaaatct ataagttatg tggtatttta tcaagtcttt
     4201 caaacccaga aactgctctg gagggatttg taaacgatta ttttcagcct aaagaaaagg
     4261 attcttatat agtttatgta aaatatgatg aaaataaaaa tattaaaaag caaaattata
     4321 aatttatgaa aaaattcaac atatcaaaag aaaaaactta tataaactgg cctctttcag
     4381 ttgaatttgg agtaaaacca ccaaaaaata tagaaaaata taatatattt gagttcttaa
     4441 aaaatttaga actaaataca aatgcaatta tattgcttgt tggagtgttt ggtgcagcag
     4501 cagtatatag tattttccat gtttcaatac ttcagagaat atcacaatat ggggttttag
     4561 aagtattagg cgctaataat aaacaacttt tatttctact atttttagag ttatttttac
     4621 tttttattgt tggatttcct ataggttgct tcttaggtat tggagtagcg tctacaatat
     4681 atgaacaatt tccacatata tttttgagta gtgatattgt tccaggagca ttttttataa
     4741 gtgaaaaatc tatttattta ggctttttat ttatcttatt gttgttaatt ttaattgctg
     4801 tacgtgttgt acatcagttg aataaatact ctagtataga atctatgaaa aactctaaga
     4861 ttactaataa gaaaaagaga atattagaaa gtgtaaaaca ctctaacatg acaaaagtat
     4921 tatctcataa atacatgact caaaaaaagg gaatgtttat tggaattttg ttttcattat
     4981 cacttggtgg aattatattt ttatgttcta gttactctat acagcttact aaaaataata
     5041 atgaacttac aatgaaagct gatgatggtt taaattctga ctaccaaata actatgcaaa
     5101 caacagattt tgatgtaggt ataccaaaag ataaaatttc aagattaaac aatgttgagg
     5161 gaatatcaaa agtctatcca gtaagatatt tatttggagc tatgcaaatt aaagaagaac
     5221 aactattttg gaaaaatttc tttaaacctc ttgaaaaaga ttacaggata aagacttttt
     5281 ttgatggtat ttgtacaaag caaaatgaag gtggctatct tttaaaaaca aatatttatg
     5341 gttataacaa tggtatgtta gaaggattaa attcttatat tatagatggg gagattaata
     5401 cccatgatat ggttaaaaat aataaggtta tagttagact acctatggat ggaactggga
     5461 tgtatgatgc agttgatata aatccaggag atacaataaa agttaaagtg ccaaagacaa
     5521 tgaaaccaac agatgaaatt gttaaattta aagaagaaaa taagaatgat tatacaacaa
     5581 aagaatttgt agttgctgct actgtaaaac gtgtgatggc aaataacata tatttcattg
     5641 gtgattatgg tatggatata gttatgacga atggtcaaat gaaaaataac tttgatatcg
     5701 aaaattataa ttcagtaagt ataaaaaaga cagaagatag taacagtgag gaattgtctg
     5761 agaacataaa aaatgttgta agtgatgtaa aaagatgtat cgtaacagat tataccattg
     5821 ctattgaaaa aaataatacc tacttaaatc aaaaactctt atttatatat ggtattgtat
     5881 ttgtacttct tgcaataagt ttgttccata taatcaatac agtaagctac ttaatttttt
     5941 caagaagaca tgagtttgga attttaagag ctatgggtat tacggataat aagtttttac
     6001 ttatgatgat aagagaaggt tttttatatg gattctatgc aagtattata atggtaattg
     6061 gaagtgttat tggacaattt atgatttatt ttatggtaaa acgtgtatac ttatatataa
     6121 atccaattct taaaataaat acacctctat acataggtat gattatttta aatatcacta
     6181 ttagtatagt tgctgtaatt attccagtaa gacaaatttt gaagagtgat atta
//
LOCUS       DAELJO010000204         6231 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELJO010000204 DAELJO010000000
VERSION     DAELJO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.206"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            225..1169
                     /locus_tag="KPB88_003316"
     CDS             225..1169
                     /locus_tag="KPB88_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG0055554.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1191..1817
                     /locus_tag="KPB88_003317"
     CDS             1191..1817
                     /locus_tag="KPB88_003317"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG0055555.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1851..2900
                     /gene="hisC"
                     /locus_tag="KPB88_003318"
     CDS             1851..2900
                     /gene="hisC"
                     /locus_tag="KPB88_003318"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0055556.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2903..3481
                     /gene="hisB"
                     /locus_tag="KPB88_003319"
     CDS             2903..3481
                     /gene="hisB"
                     /locus_tag="KPB88_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG0055557.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3483..4088
                     /gene="hisH"
                     /locus_tag="KPB88_003320"
     CDS             3483..4088
                     /gene="hisH"
                     /locus_tag="KPB88_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG0055558.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4085..4807
                     /gene="hisA"
                     /locus_tag="KPB88_003321"
     CDS             4085..4807
                     /gene="hisA"
                     /locus_tag="KPB88_003321"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG0055559.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4789..5547
                     /gene="hisF"
                     /locus_tag="KPB88_003322"
     CDS             4789..5547
                     /gene="hisF"
                     /locus_tag="KPB88_003322"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG0055560.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5575..>6231
                     /locus_tag="KPB88_003323"
     CDS             5575..>6231
                     /locus_tag="KPB88_003323"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG0055561.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     K"
BASE COUNT         2536 a          534 c         1237 g         1924 t
ORIGIN      
        1 ttaaatacaa aattaaatca tcaccatcat catagattaa agggattgta atatgaaatt
       61 aattcataga cacaagccct gtttttagtg taattggtct tgtatctatg atgggaagta
      121 attttaagag aaaattatag ccatataggt ataaagccta tatggctttt ttattgtttt
      181 aaatttgcaa atataagagt aaatttaatg aagagggtga gaatgtgaaa tttttgagga
      241 ttgaagatga ggttatttat tctaaaaaaa gatataaatt aaaaagggaa atagataaac
      301 tttttttgga tgaagaatat tttcaaatag aaccacaatt atttgaagaa tatgatgaat
      361 ttacaactat aaataataaa attccagaag aatctatggt aaaggttgta aatggaaaag
      421 taatggtact tagagctgat ataactacaa gtataataaa aagccttgta ccaagatggg
      481 aagatggttt aaagcttaag cttttttata attcatcaat atataaaaat aaaaatacag
      541 ttggaataaa agagataaga caaataggtt gtgagtatct aggagaagcc tctgtagaag
      601 cagatagaga agtagtgaaa ttagctctta agatacttga aaaatacaat aataatttta
      661 ttctagaggt tgggagtagc aagtatattc atggtttgtt agaagaatta aatttaaata
      721 agaattgtga aaatcaaatt aaaaatttac tatatacgaa gaacactcat gaactaaaag
      781 tatatattga agaattaaaa attaagcgtg aagtaaaaga gttattatca aatattttaa
      841 gtttacaagg gaatttatat aatgttattg aaaaatcaaa caagttctat tgtaataata
      901 agatgaagca agctcttgaa gaattaaagc aagtaaataa tttaattgag gaatgtaatt
      961 ttttagataa ggctagattt gatttatcaa tgattactat gcttgactat tatgaaggaa
     1021 ttatgtttag aggatactac ccaaactcat ataaagaaat tcttagtggc ggaagatatg
     1081 attctttaac taaagagtat ggaaaagaaa ttccagccat tggatttaca ctaagtgttg
     1141 atgagttaat gaagtatgta tataagtaaa gtttataagg agggattgtt ttggattatt
     1201 tgactatagc actagcaaaa ggacgaattg aaggggaatc atttaaaaaa tttaaaaaaa
     1261 tgggtctagg agatagtata gacacagata cgagaaaact aatttttaaa gatgaagaaa
     1321 ataaaattat atatatacat gtaaagccat cagatgtagt tacatatgtt gaaaaaggag
     1381 tcgctgacct cggtatagca ggtaaagata ctattttaga aaatgagaca gatgtatatg
     1441 aaatatatga tttaggtttt ggaaagtgta aatttgctgt agcaggatta aagggagata
     1501 gcatatatag agaagatgaa tatttaaaag tggcaacaaa gtatcccaat atagcaaaaa
     1561 agtattttaa agagaaaggt caaaaaattg aaataataaa attaaatggt tctgtagaac
     1621 tagctccaat agtagggttg tcagatgtaa ttgtagatat agttgagact ggcaatacac
     1681 ttaaagcaaa tggtcttgaa atattagaag acatatgtaa tataagtgct agaattatat
     1741 caaatagagc tagctataga tttaaatatg aacaaattca gaatataatt cgtttatttg
     1801 aagaacttga taattaaaat ataattgagc tttataagga gggatttaag ttgagagaaa
     1861 aagaaagcat aagagagtta aggggatatg aaccaaatca cgtaaactgt aaagtaaaac
     1921 ttgatgcaaa tgaaggaagt aagaggttgt ttaaatacct tattaaagaa atttcagata
     1981 gtgatataga tttaaattta tatcctgaag attcttatag tgatttgaaa gaatctataa
     2041 tagattacat aaatatttct ggagtaaata agaaaaatct tctagtagga aacggttcaa
     2101 gtgaaataat tgatttaata atacatactt ttgtagataa agatgaagta atattgagtt
     2161 tctctccaag ttttagtatg tattctatat atagtcagat aaatgggtct aaatttatag
     2221 gtgttgaaag tgatgaaaat ttagttataa atatagataa tgtgattgaa aaagtaaagg
     2281 agaataatcc taaaatagta attgtatgta atcctaataa tccaacggga acaatattaa
     2341 agagggaaga gataataaag cttttagatt caactaatag tctagtggtt ttagatgaag
     2401 catatatgga ttttggagaa gaaagcatgt taagtgatgt ttttaagtat gataatctaa
     2461 tagttcttag aactttatca aaggcatttg gattggctgg aataagaaca ggatatatgt
     2521 tatctaatag tagtttaata aattctgttg agaaagtaag acctccatat aatttaaatt
     2581 cattatcaga ctttattgct acaagagcac ttagaaataa agatgtggta aaggcttata
     2641 taaaggaagt aaaagaggaa agagaagttt tatatgaaga aatgattggt atgggaatta
     2701 aagcatacaa atcacaagca aattttatat tattttattc tgaaatcgaa aatttatcac
     2761 aaaaacttat tgatagaggt gttttaataa gaaaatttgg tggaaagtta gaaaattatt
     2821 acagagttac aattggtgat aaagaagaaa attctatgtt tgttggagct ataagagata
     2881 ttttgaaaaa ggagaaatag gtatgagaat ttggaaggta gaaagaaata ctcttgaaac
     2941 acagatttta gtagaattaa atattgatgg ttctggtaaa gctgaaatag atactggaat
     3001 aggattttta gaccatatgc ttaccttaat gtcatttcat ggcaagttcg atttaaaagt
     3061 gatttgtaag ggtgatacat atgtagatga ccaccatagt gtagaagata taggtatagc
     3121 tataggtgaa gcatttaaaa atgcactagg tgacaaaaaa ggaatacgaa gatattctaa
     3181 tatctatata cctatggatg aatctctatc aatggtagca atagatataa gcaatagacc
     3241 ttatcttgta tttaatgcta aatttgatac ccaaatgatt ggtagtatga gtactcaatg
     3301 ctttaaagaa ttttttagag cttttgtaaa tgaatcgaga gtaacattac atatcaatct
     3361 cttatatggg gaaaatgacc atcataagat agaaagtatt tttaaagcat ttgcaagagc
     3421 tttaaaagag ggtagtgaaa tagtttctaa tgagatagca tcttcaaagg gggttttgta
     3481 gaatgaatat aatagtagac tatggtcttg gaaatataga ttctgtgtct aggggtttta
     3541 gaaaagctgg aatagaaact aaaatttcta gtgatataga tgaaataaaa caggcagatt
     3601 cacttatact tccaggtgtt ggagctttta gagattctat aagtgcctta gataaactag
     3661 gattgatacc aataataaag gagcatgttt ctaagggtaa atttatgata ggtatatgtc
     3721 taggaatgca attactttat gaaaaaagtt atgagtatgg agaatatgaa ggattaggac
     3781 ttataaaagg aagtattgat aaactggata taagtttaaa agtgccacat atgggatgga
     3841 ataatctaaa atttaataag gcaaatgatg atatacttaa atatataaat gaagatgatt
     3901 atgtatactt cgttcactca tattatgcaa attcatcaaa tgaagaacta attgcatttt
     3961 cagaatatga aaagaaaatc cctgctattg ttagaaaagg caatgtatat ggtatacaat
     4021 ttcatcctga aaaaagtgga gaagtagggt taaatatatt aagagcatat ggggagatga
     4081 taaaatgata atatttccag caatagatat aaaagataat aagtgtgtaa gactcacaca
     4141 aggagaattt gacaaagtaa atgtatatta tgataatcca ttagaagtag catataaatg
     4201 gaaaaatgaa ggagcagaat atatacatat agtagattta aatggtgcta gaagtgaatt
     4261 tggtgtaaat acaaaaataa ttgaggacat agcaaacaat atagatatac ctattcaagt
     4321 tggcggtgga gttagggata aggaaaaagt caaaagttta ataaatgctg gtgttactag
     4381 agtaatatta ggcagtatag cgattgaaaa tttgaattta gttgaagaat tggtcaatga
     4441 atataaagag aaaattgtag tttctataga tgctaaagat ggaaaagtag cagttagggg
     4501 atgggaagtt gtaagcaatg tagactcatt aacattatgt aaacaacttg aaaagatagg
     4561 tgtacaaact attgtttata cagatatatc aaaagatggg atgttgcaag gtccaaattt
     4621 tgacatatat gaaagaatag caaaggaaac atcactaaat gttattgctt ctggaggtgt
     4681 aacttctata gaagacgtaa aaagactaaa agctatgaac ttatatggtg caataatagg
     4741 taaagcgctt tatgataaaa agatagactt taaggaggca caacaattat gcttactagg
     4801 agaataatac cttgtctaga tgtgagaaat ggaagagttg taaaggggaa aaaatttaaa
     4861 gatatagtag atgttgatag tccagaagta cttggaaaat tttatagtga ttgtggtgca
     4921 gatgaacttg tattctatga tataactgca tcaaatgaag agagaaagac atctttagaa
     4981 tttgtgacaa aagttgcaga aaatataaat ataccatttt gtgttggtgg aggagtaaat
     5041 aaattagaag atttcactga tattttaaga aaaggtgctg ataaagtttc tataaattca
     5101 tcagcagtca aaaatcctga attaataagg gaagcttcat taaaatttgg agcacaatgt
     5161 gttgttttat cgatagatgc aaagaaaaat gaagagggtt catggagcgt atacgtaaaa
     5221 ggtggaagag aaaaaactaa tctggatgca attgaatggg ctgttaaagg tgtagaactt
     5281 ggagcaggag aaatcgttgt aaatagtatg gatgaagatg gaatgaaaaa tggatatgat
     5341 atagaattat tatcaaagat aacttcttta gtaaatgtac ctgtcatagc atctggaggc
     5401 gctggaaaaa aggaagattt ttatgaggcg gttaataaat ccaatgtaga tggaatttta
     5461 gcagcatctg tttttcattt tggcgagata aaaataaacg atttaaagaa atatttaaaa
     5521 gacatgggtg tagaagtaag attataagtt aaatactaaa aggagattag aaatatggat
     5581 aataagtgta acaatgtata cagtgatgaa gtagagaaat ttataagaag tataaagttt
     5641 gatgataaag ggttagtccc tgttgttgtt caagaggtag ttagcaaaga tgtactgatg
     5701 ttagcatata tgaataaaga tgcgataaag aaaaccttaa aagataaagt agcttgctat
     5761 tttagtagaa gtagacaaga actttgggtt aaaggggaga catctggtaa tactcaaaaa
     5821 gtagttaaga tgtcttatga ttgtgatgta gatactatac tactttttgt tgaacaaact
     5881 ggtgtagctt gtcatacagg caattattct tgtttttaca gagacttgtt tgatgatagt
     5941 tcaaaaatgg agcttgaagt acaaacaaat atattgaaag aattatatga tttaatcaat
     6001 gaaagaaaaa ataatccagt tgaaggttct tatactaatt atctttttga aaaaggaata
     6061 gataagattc ttaaaaaagt aggtgaagag agtagtgagg taattatagc atcaaaaaat
     6121 accgataaaa gtgaacttat ttacgaaatt agcgatttag tatatcatac attggtttta
     6181 atgatagaaa aaggtgttga aattgatgaa ataaaaaaag aattacttaa a
//
LOCUS       DAELJO010000205         6226 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAELJO010000205 DAELJO010000000
VERSION     DAELJO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.207"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..434)
                     /locus_tag="KPB88_003324"
     CDS             complement(<1..434)
                     /locus_tag="KPB88_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055562.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENG"
     gene            complement(504..731)
                     /locus_tag="KPB88_003325"
     CDS             complement(504..731)
                     /locus_tag="KPB88_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055563.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            855..1172
                     /locus_tag="KPB88_003326"
     CDS             855..1172
                     /locus_tag="KPB88_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055564.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            complement(1152..1340)
                     /locus_tag="KPB88_003327"
     CDS             complement(1152..1340)
                     /locus_tag="KPB88_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055565.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(1417..2028)
                     /locus_tag="KPB88_003328"
     CDS             complement(1417..2028)
                     /locus_tag="KPB88_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0055566.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            complement(2028..2201)
                     /locus_tag="KPB88_003329"
     CDS             complement(2028..2201)
                     /locus_tag="KPB88_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055567.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            complement(2930..3436)
                     /locus_tag="KPB88_003330"
     CDS             complement(2930..3436)
                     /locus_tag="KPB88_003330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055568.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSTNIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            complement(3541..3717)
                     /locus_tag="KPB88_003331"
     CDS             complement(3541..3717)
                     /locus_tag="KPB88_003331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055569.1"
                     /translation="MKKFIYINRHRVYIRNLFKISAIYKENHYIREFKSECLSNNTLN
                     AVIESSVRIGFGSK"
     gene            complement(3740..4183)
                     /locus_tag="KPB88_003332"
     CDS             complement(3740..4183)
                     /locus_tag="KPB88_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0055570.1"
                     /translation="MKVNFTIDGEPKAKARPRMSTANGKAYTPDQTILYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKNAIDKVEGLIRPNKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDRPRVEVELEDVS"
     gene            complement(4173..4571)
                     /locus_tag="KPB88_003333"
     CDS             complement(4173..4571)
                     /locus_tag="KPB88_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055571.1"
                     /translation="MQKDVWLYSWDNKYLSSDEYESKEEAIQAAKEELKEFGNFGESI
                     YVGKKEEVSIPNIDIEEALECIQKKIDDEVGECGEDWFENICVEDMIILSNRVNEVFE
                     KWIDEFGYKPYWFKLTDKEEIELNEVANES"
     gene            complement(4725..4955)
                     /locus_tag="KPB88_003334"
     CDS             complement(4725..4955)
                     /locus_tag="KPB88_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG0055572.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLEIRKEIIYLLDNSNYLQDGYVVLGIK"
     gene            complement(4978..5250)
                     /locus_tag="KPB88_003335"
     CDS             complement(4978..5250)
                     /locus_tag="KPB88_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055573.1"
                     /translation="MITTQMYKINNEIFLNDLCLEENKEMTIWVEKNKDTHEFIWLKI
                     ANVNGKLAIFIQDIENAVIKEWQGQIAYKKVIYQIEYEQVEKGEID"
     gene            complement(5335..5502)
                     /locus_tag="KPB88_003336"
     CDS             complement(5335..5502)
                     /locus_tag="KPB88_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055574.1"
                     /translation="MTNKEMCKSKNLDEREIYKEFGKEICASCISDRVDCESKDCDIA
                     YENWLEKDAER"
     gene            complement(5511..>6226)
                     /locus_tag="KPB88_003337"
     CDS             complement(5511..>6226)
                     /locus_tag="KPB88_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0055575.1"
                     /translation="SCDLAICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMS
                     KNEINIQETKTVKIAALVGLSGMNDEYHLRVLDKDAEKEEANSKFITEFLNATKVKDD
                     KYKTKMFKSTAENWITNALGNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKL
                     KDSFKEHMEEKGLDKGFSIDKKWVEKKLKKRNIKTDNGFDIKGNLTDFEDPMKYTVRQ
                     NQNGSIDIIIKNVKFYEEK"
BASE COUNT         1864 a         1152 c          600 g         2610 t
ORIGIN      
        1 tcaccatttt ctgtgctttc atatttcttt aattctttaa ccatttcttc cttgccttta
       61 acatacatta tcttttgtgc tcttattatt gctgcatatt gaattgttat ctgttcccaa
      121 agaatatcaa atttatcttt tatagatatt tcttgtatca attccctagt ttcttcgggt
      181 agatattttg agaagaaacc aaacttttca gcgttcttat tctcttttgg agcaccatga
      241 ccaactgaat ttttattaga aaagggtgca cctcttttat ttataggtgc acccttcttt
      301 ttttcactag cccagttgta tctttttatc catgacttta aagtgtttaa gctaatgtca
      361 tactttgatg atatttcctt ttgtttcatt ccttttaagt aatcttgttt taccttttct
      421 ttgacatctt gcacatcacc acctctttat ttgtttgttt tgggaataaa aaaagaacct
      481 cataattgag attctttttt tgtttactct actttaagat tcaattcaaa ttcagtccca
      541 cattctaaac atttaacttt accatcttct agtatttcaa attcagatat tttttcgcat
      601 attttacatt tttctttgaa cttatttcca acattttgtg atgtaaatat agtagggtta
      661 ttttcaactt ctttttttaa atcatcaata acactgtcaa aattcaaatt accacttatc
      721 tcaaatccca cataatcacc tccttatagt atagtaaatt ctatgttatt atacaatatc
      781 cttcaacaat cgttcgacaa tagcagaatt caccacaatt ttatgctaat attctattga
      841 aaggaggtgt tcttatgagt aaaaatattg acattagaaa tttaaagcaa tttcaaaaga
      901 aaattgaaag aatggaacaa aatttaaaac ctgactttga taagattcgc acaataattc
      961 ttagcaagaa atttatgaac caacacacta attttgattc ttttgatgaa ctacttgcat
     1021 ttggtaatta catagtcaat tcagaagaag actttttagc tattcctgat aatgaatttg
     1081 atttatttat agttaaaaat actgatttcc cagattggca aaccatgctt gattcagcat
     1141 attcaaaata tttagagtct tgccttagat aaaacatcta gacaactttc aaaatgttta
     1201 tctaattctt ttatgatttc cctagattcc tttataaact cagccatata acacacacca
     1261 cttgcaggta tcttgaattc agaatctagg gaattttttc tttctgatac tacatcttgt
     1321 gtgtttttct taatctccat aatttcattc tccttttaat ttattgtata aaaaaagacc
     1381 atctatcaag atagtcattt tagattttat gtacttttat ttttttagat atttcaacat
     1441 ttgaactaaa tagtccccat ctaacgaatc tgtttcgtct ttatcaaggc ttctttttcc
     1501 aaagtctaca aatccattat ccttataaaa ttcaattaat ttaggtttat cttcacattc
     1561 aaggtacact attttcccac ccatatctaa ctgtactgct tttatcttgt cacatgcaat
     1621 ttttagaagt tcatcaccct tgattaattt attataatta tttgaataat tctttcctat
     1681 ttgccctatc aaaggtgctc caataatata tcttcttagt tcttcattgt attgtccaaa
     1741 cttcactatt ttcctagcta aagagtttga taatgttttt ctttttattg taaaatactt
     1801 attagctaga gtgaaatatc caactataac aggcttgcct ttataagaag ttagcactaa
     1861 atgtgtacta gccaaacctt gtttagaaaa ttcaatagct ttgtttttta aaaattcttc
     1921 tacatcttta ttaaggggac aagaaaaact ggagagaatt tttttaactt cttcttcctc
     1981 cagctctcct agcatattac ttaagtttac aattaaatag ccactcatta aaatctcccg
     2041 aatatatcct ttattttatc ttttggtact tctgaacatt ttttacttaa tacaacttct
     2101 ttttcttgtt tatttttagc attttctaga gctgatacta gatttcttcc aaatgccttt
     2161 tttcttacat ctacattttt taaaatactt tttgtagcca taagtaccac cttccacttc
     2221 actattataa tttaattata ctgacgttac gttaaatatg caatacattt cgaggaatta
     2281 tcagttggca ttttgtacat gattttatta ttattattca caatatgtac attttatata
     2341 ataaaattac tgttatttat aattaattta aataacttac actataaaaa attatgtcat
     2401 atatcttatg tatattaatt caaatgcttg ctaagtattt aattttaagt atgcacatta
     2461 taatatacct aaacaaatag tattatgaca ataaaaattt cttttttctt tttaaaaagt
     2521 aaaatgctaa gtttggagta aacttagcat ttttagtagg gagatacata tattatgtcg
     2581 caagttctag gaatcgaacc tagattaaac accagcacct acatggtgag tgaggttacc
     2641 aagccccact cggtttttag acttctgaat taagatacaa aattgtatga gattttaatc
     2701 ttaattcaac tactacatat agtgtattaa taggttttga acatagttag aattgaacta
     2761 acagcgtcct cacgccctgc ctagtctgtt cgtagtgact agggcaatcc cttaacccta
     2821 gtcaaatatt aagttttgag agggaaatct ttatttccac aatactatta tctcatgtct
     2881 aaaacaaaaa aacctgcaca ttttcagcac tcaaaattac tcttactttt taatttagtt
     2941 tgtaatctaa caattcaaaa agtggttctt gctctattaa tgctttcttc ccaaacagtg
     3001 ctattgatat tgagctaata gcttgactag ctcttacact taattgtctt tcttctaaat
     3061 gtactatatc aaccatttct tgccacatta gaccatctat atatttaagt tcaataattt
     3121 gtctgtgtat aggttttaaa tttcttatgg ctaaatctat tgtagattta attatttctg
     3181 cttcatataa ctctatttct ttttctgtta ttaagtctga tacattaaca atagcatcct
     3241 caatcatatt gctagttttg tttgttttac ctgttttgac actatcataa cttatccctt
     3301 tcattaaatc cccaaccgaa ttatctttca acatttgtat ttcatttttt agttttatta
     3361 tatttgtact taattgttta taattagaaa gttgtttctt agttgcatta aaaaactctt
     3421 ttttagtttt agacatactc acacactcct atcaattatt tatgttataa taatatttgc
     3481 atataaaagt tttatatttt tgacaagtag gagcgtgaag taatgctcct ttttttcttt
     3541 ttacttacta ccaaatccta ttcttacaga agattctatt actgcattta aagtattatt
     3601 agataaacat tcacttttaa actctctaat ataatgattt tctttgtata tagctgatat
     3661 tttaaataaa tttcttatat atactctatg cctatttata taaataaatt ttttcatatt
     3721 attaacctta atattctcct taactaacat cttccagttc aacctcaact cttggtctgt
     3781 cactataata tttcttactc actacttcta ctatttgaga atcatcttta taagctatac
     3841 catttaaact gtcagctaca gacttaatta tattgtctaa atcgggtttc ttatttggtc
     3901 ttattaaccc ttctaccttg tctatagcat tcttatacgc tttagtattt accttatttt
     3961 tctgtagtgc ttctctatct tttttagtaa tgtcaaagta acaaataact gtcattttca
     4021 cattaccctc aaagaaatgc tttactgtag agttatacat gagtctaatc cagttttcat
     4081 ataatatagt ttggtcaggt gtataagcct taccatttgc tgtactcatt ctaggacgtg
     4141 ctttcgcctt tggttctcca tctattgtaa aattaacttt cattggctac ctcatttagt
     4201 tctatttctt ctttatctgt aagcttaaac caatatggtt tatatccaaa ttcatctatc
     4261 catttttcaa atacttcgtt taccctatta ctaagtatta tcatatcttc aacacatata
     4321 ttttcaaacc aatcttcccc acactctcca acttcatcat caatcttttt ttgaatacat
     4381 tctaatgctt cttctatatc tatgttaggt atactaactt cctctttttt accaacataa
     4441 atactttctc caaaatttcc aaactcttta agttcttctt tagctgcttg gatagcttct
     4501 tctttacttt catactcatc actacttaaa tatttgttat cccagctata taaccaaaca
     4561 tctttttgca tattaatacc tccactattt atttttctta ttagccttct ttctacagtc
     4621 cttacaacaa taaatctcct tagatttttc cttaagataa aacagcttgc cacaccaact
     4681 gcatcttctt cgtttcataa actcacttcc tagtcacaaa acatttattt tattcctaaa
     4741 acaacatacc catcttgcag ataattgcta ttatcaagta aataaataat ttctttgcgt
     4801 atctcaagac ctgtgtattt ttctccatca aattcctgca acaccaacaa gtcgcctttt
     4861 ttaaaacctc tatcattctt cctaacttca aaatttttgt tcccatttac aacttcttta
     4921 aaatattgag gtaatatttt taattcatgt atcataagct cacacccttt aaaagtttta
     4981 gtctatttca cctttttcaa cttgttcata ttctatttga tatataactt ttttatatgc
     5041 tatttgacct tgccattctt ttattactgc attttcaata tcttgtataa atatagctaa
     5101 cttgccattt acatttgcta tttttaacca tataaattca tgtgtatctt tattcttttc
     5161 tacccatatg gtcatttcct tattttcttc taaacataaa tcatttagaa atatctcatt
     5221 atttatctta tacatttgag tagtaatcat aatctcacct ccttcctata agtcaaagta
     5281 agtctataac attctagttt cattcacaaa cttaccttga ctttattttt ataattatct
     5341 ttcagcatcc ttctctagcc aattctcata tgctatatca caatctttac tttcgcaatc
     5401 taccctatcg cttatgcaac tagcacaaat ctctttccca aattccttat atatttctct
     5461 ttcatcaaga ttctttgact tgcacatttc tttattagtc atatgctcac ctacttttct
     5521 tcgtaaaatt ttacattctt aataattata tctatagacc cattttgatt ttgtcttact
     5581 gtatatttca ttgggtcctc aaaatcagtc agattacctt ttatatcaaa gccattgtca
     5641 gtttttatat ttctcttttt aagctttttc tcaacccatt ttttatctat actaaatcct
     5701 ttatcaagac ctttttcttc catatgttct ttaaagctat cttttaactt atcatcttta
     5761 attgttttat caacaaaatt atttatatca atttcatgct tttctttcaa agtataattt
     5821 aatatacttc ttacatcttc tgcctgtttt atatcatttc caagagcatt agttatccaa
     5881 ttttcggctg tacttttaaa catcttagtc ttgtacttgt catctttcac tttagtagca
     5941 tttagaaact ctgtaataaa cttagaatta gcttcttcct tttctgcatc cttgtctaaa
     6001 accctaagat gatattcgtc attcattcca ctcaaaccaa ccaaagcagc aatttttact
     6061 gtcttagtct cttgtatatt aatttcattt ttagacattt gtatgttaaa tttatcatct
     6121 ttaaactcga ttgaatgagt atatgacttg ttataatcaa gttttaatat agcaactttc
     6181 ttttcatctt tttgagagta taagcaaatt gctaagtcgc aagatt
//
LOCUS       DAELJO010000206         6202 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELJO010000206 DAELJO010000000
VERSION     DAELJO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.208"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            248..1255
                     /gene="asrA"
                     /locus_tag="KPB88_003338"
     CDS             248..1255
                     /gene="asrA"
                     /locus_tag="KPB88_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG0055576.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYLYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIASGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     V"
     gene            1256..2047
                     /gene="asrB"
                     /locus_tag="KPB88_003339"
     CDS             1256..2047
                     /gene="asrB"
                     /locus_tag="KPB88_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG0055577.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2060..3031
                     /gene="asrC"
                     /locus_tag="KPB88_003340"
     CDS             2060..3031
                     /gene="asrC"
                     /locus_tag="KPB88_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG0055578.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3088..3864
                     /locus_tag="KPB88_003341"
     CDS             3088..3864
                     /locus_tag="KPB88_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0055579.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4135..6153
                     /locus_tag="KPB88_003342"
     CDS             4135..6153
                     /locus_tag="KPB88_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0055580.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT         2493 a          551 c         1136 g         2022 t
ORIGIN      
        1 cttttttatt caaaaaattg agtagatagt agttttattg tacttaatga ttgtatttaa
       61 gtgattagat ttgaaatcga ataatgtaga taataaaaaa atattaacag aacttaataa
      121 attccattaa tattttttta ttatttttga aactgtgata attatcacgg aagcatagaa
      181 aataaagtgt taagatttaa acaagatagc aagtaagtaa aactaaaata taagaaaggt
      241 gataaaaatg aaactaaaat tagattcatc taagttcaat gaaggtctga attatctaaa
      301 aaaagactac aagatatttg caccagtttt aatcccattt aaaggtactt tttcagatac
      361 tgatgttata agatacaagg aggtaaacgc ttttgaagaa atggaatttg ctaagaaagc
      421 aaacttttca ccaaaagaag ttgtacttcc cataaatcaa gtattgtttt attttactga
      481 aaaagagttt aaggaaagcg atttagacaa caagaaaatt ttaattttct taagagcttg
      541 tgacataaat ggtataaaaa gacttgatga aatatatcta aataatggtg aagaaaaaga
      601 ttatctttat aaaaatatca gagataaagt aaagtttgca cttgtagggt gtaaggaaag
      661 ttttagaaac tgtttttgtg tgagcatgga ttctaacaaa actgataatt attcaattgg
      721 attaaatata gaaggagata cactgtactt agatataaag gatagtgaat ttgaagtatt
      781 taatggagaa gcaactgaat ttaatgttga ttatgtaact gataatttaa tatctgtaga
      841 tgtgccagag aatattgatt caaatgaaat gataggaaat tcaatatggg atgaatatga
      901 tacaagatgt ataggatgtg gtagatgtaa ttttgtatgt ccaacatgtt catgttttac
      961 aatgcaggat attttctata aggaaaacga aaatgtaggt gagagaagaa gggtttgggc
     1021 ttcttgtcaa gtagatggat atacagatat agcaagtgga aattctttta gaaaaaaaca
     1081 aggcgaaaga atgagattta aaactatgca taaaatacat gattttaaaa aaagattcgg
     1141 atatcatatg tgtgttggct gtggaagatg tgatgatgcc tgtccgcaat acatatcatt
     1201 ttcaaagtgt atagaaaaaa taaatgattt agtaactagt aaggaggaag tataaatgaa
     1261 cccatatata ccagtaagtg caaaaatact agatatagtt aaacatactg ataaagaatg
     1321 gacttttaga gtaaactgca atacaaaagg tgtattgcct ggaaagtttt atgagatatc
     1381 tatacctaaa tacggtgaaa gtccaatatc agtatcagga tatggagagg attatattga
     1441 ctttacaata agaaatgtag gaaaagtaac tagcgagttg tttaattata aagaaggaga
     1501 ttcttttttt ataagaggtc cttatggaaa tggatttgat gtatcccttt atgaaggaag
     1561 agaaattgta gtggtggcag gaggaagtgc attagcccct gtaagaggaa tagttgaata
     1621 tttttataat aataaagaaa aatgcgaaaa gtttaaatta atagtaggat ttaaatcacc
     1681 aaaagatatt ttatttgcag atgatttaaa gagatggagt aaaaaacttg atattttagt
     1741 tactgtagat ggagcagagg aagggtatag tggtaatgta ggattagtaa caaaatacat
     1801 accagaattg gatatgaaag atataaacaa tacttctata gttgtagttg gacctccaat
     1861 gatgatgaaa tttacagtag aagaattttt aaaaagagat ttagatgaaa agaacatttg
     1921 ggtttcttat gaaagaaaaa tgtgttgtgg aataggtaaa tgtggacatt gtaaaatgga
     1981 tgatacttac atatgtttag atggaccagt atttgattat tcatatgcta aaaatcttat
     2041 tgattagggg gcgaagtttg tgataagaga tttaaatacc aaaaaagtaa tgaaaaatgc
     2101 ttatagaatt acaaaaacaa aatatgagac ttctttaaga gttagagtac ctggtggtct
     2161 tattgaccct gaaagtttag ctatagtttc taaaattgca agtgagtacg gcaatggaca
     2221 ggtgcacata actacaagac aggggtttga aatattagga ataaatatgg aagatatgga
     2281 agaaataaat aaaattatac aacctgttat tgaaaaaatg aatataaatc aaagtgagaa
     2341 aaacgcaggt tatccagcgg caggtacaag aaatatagca gcttgtatag gaaataaagt
     2401 ctgtccaaag gctcaatata atacaactga atttgctaaa agaatagaaa aagctatatt
     2461 tccaaatgat ttacatttta aagtggcttt aactggttgt ccaaatgatt gtataaaagc
     2521 tagaatgaat gactttggaa ttattggtat ggctttacca atatatgaaa aggatagatg
     2581 tgtaaattgt ggagcatgtg taaaaaaatg ttctaagata tctgttggag cattaaaaac
     2641 agaaaataat aaggtggtaa gagataaaga taagtgtata ggctgtggag agtgtgtact
     2701 aaattgccca acaaatgcat ggactagaga tgagaaaaaa tactatagat tagctataat
     2761 gggtagaaca ggaaagaaaa accctagact agcagaagat tttcttttat gggtagatga
     2821 ggacagcata ataaagataa tacttaatac ttatagatat gtagaaaagt atatagataa
     2881 agatgcacca ggaggaaaag aacatatagg atatataatt gatagaacag gatttatgga
     2941 atttaaaaaa tgggcacttg aaggtgtaga gcttccagaa ataactaaaa tgtacgaaaa
     3001 catatactgg agtggaatta aatatctata aattgagtaa aacatatatt tgaataaatt
     3061 aaaatttaat agaaggaaga gggaattatg cataaagaaa ctttggataa attaactaat
     3121 gcagctataa ataagataaa tttattaaac acaagtaaag taaaatattt ggtatcttct
     3181 gcgtttgcag gactttatgt aggtataggt atacttttga tatttactat tggaggactt
     3241 ttgactgatg caggttcacc tatgactaag atagtaatgg ggttatcatt tgcaatagca
     3301 ctaagtttgg ttataatgac gggaacagag ttatttactg gaaacaatat ggttatgtct
     3361 gctggtatgt taaataaagg tgtaagtata aaagatactt ctaaaatatg ggcatacagt
     3421 tgggtaggta atttaatcgg agctttggtt ttaggcatta tatttgtagg aactggtcta
     3481 gtagacaaag gacctgtagc tgaatttttt gcaaatacag cagctagtaa agcatccatg
     3541 ccatttacag ctcttttctt tagagggatt ttatgtaata ttctagtatg tgtatcagtg
     3601 ttgtgttcgt ttagaactaa tagtgatact gcaaaaataa ttatgatatt cttatgttta
     3661 tttgctttta taacatcagg ctttgagcat agtgtagcaa atatgacaat ttatagtgtt
     3721 tcattatttt caccaacaat aaatacagtt actattggag gagctattta taatttagta
     3781 gctgtaacac ttggaaatat tgttggagga gctttattta tgggtcttgg aacttatata
     3841 ttaggtaaag aaaagcttaa ttaaatttat tgtataaatt ataactctct tgataaatac
     3901 atttttagta ttgttgagag agttattttt atatttttga aaaaatattt gaaacaaaaa
     3961 aaatatggga gcatacgaaa atatatcagt tatatatata aaaaatacgt ttatatatga
     4021 attatatagt tctaaatata aaaaaagtta aagtcctacc gtaaaaaagc gcattttgaa
     4081 taagataaca aatatgataa aattggtctt gttgaaattt ttagggggga tatgatgaca
     4141 aagaaactag taatatcaaa aacaaagttg tttattttta taatagcatt tatttctata
     4201 tttatatatt tatttggaag taaaaataca cttataggtg taggtatagt aacagctatg
     4261 ctaacactct tggaaagaga tttaactatt tctccaatta aaaatttact gaagtattta
     4321 gcaataaata taatcttagg aatactatca ttttttgcag tacaaaatat gtatttagga
     4381 gttttattaa attttatagc attatttata atagggtatg tatttagtta tgacttaaaa
     4441 agagcagtat atgtaccctt tggtctaatg tatatattta tggtaagtat acctgtagga
     4501 aaaagtgaat ttcctatgag attgtcggct ttagctgttg gagcagttat aataatgatt
     4561 gctcaatttg ttatgaatag aaataggatg aaaaatgtcg gagataaaga actgatatct
     4621 atatgtgatg aattattaga aaaaattagt cttttaaaaa atactattaa tgatgatagt
     4681 tctataatta aatctagtat gagaaaaata gatagctgta attatagaat aaattctata
     4741 tctaaaaatt tgaaaatggt tatttttgat aatagaaaag atgactttta tatcagtatt
     4801 agaggtatag acataatgaa tattttattt tcattagaaa gaataagttt aatcctggaa
     4861 agatacaaaa aggatagtaa agaatttgaa gatgaagata taaaaaatat tttagaagaa
     4921 agtagcataa atagtaaatc agatgtttta gttgtagcaa cgaaagaaat aaactatatt
     4981 aagatgtgtt tagaaaataa agataccata actgataaag agatattagg atttagagat
     5041 tatgttattg ctcaggatac taaaaatata aatttaaaag aaatttatag tgttttagaa
     5101 aatttatatg agtttttagt agattataaa aaagtaaaat caagagacga aaagaaagct
     5161 gaaagaaaat caaagatacc tcatgaattt aaacgactat caatatataa gaaaaacttt
     5221 aatttaaatt ctataagatt ttcttatgca gtaaagattg ctttagctac agctgtagcg
     5281 ggttttatta tggattattt ccatttaaga gatggaagat ggataatgtt aactgtcttt
     5341 tcattaacac aaccttacgc agaaaattgt atacaaaggt ctagaaaaag aatagaaggt
     5401 acatttatag gagctgtaat atttattgta ttattttcaa taataaaaga tagtacatta
     5461 aggtcgttaa tagttctttt agcagggtat ataaactctt atgtggttga ttatagaaaa
     5521 ttaatggtat gtgtaactgt ttcagctctt ggttcagcag tagttatggg agacccaaat
     5581 gttttaacca taagcaggat ttcatatgta gcattaggag caattattgc attaatagta
     5641 aataagttta tattaccata tgatgctaaa acaggctatc aacatgttat agaaatgtat
     5701 aaaggaatag ttaaaaatat aattgatgaa gtcaataagt ctatagaaaa tgtagctgat
     5761 gtttactata taaaaaattt attattgata ccatcactta ttgaagatag acttatgttg
     5821 ataaatgcta tatataaaga taaacatcaa gaggattttt tagaaaatca gaaattacta
     5881 ataagtaata tgtataatct atatataaat gttaataaaa ataaaataaa agatgaagat
     5941 gttgaaaaaa tattgagaga tactaattat atttcaaatt acaatgtaga taagtatgat
     6001 gaaggaagaa gtgtaatttt agagagtatt gtaaatacta aaagtctagg tgacaagata
     6061 atatgtctaa acttactgca aactctaaat ggtgtcaaag aaatgtatag aataagtaat
     6121 ataactaaag tttcaataaa agaagcagct tagatattaa attatagaat agccatatat
     6181 ttaaaataaa gtatatggct at
//
LOCUS       DAELJO010000207         6107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELJO010000207 DAELJO010000000
VERSION     DAELJO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.209"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            243..1055
                     /gene="modA"
                     /locus_tag="KPB88_003343"
     CDS             243..1055
                     /gene="modA"
                     /locus_tag="KPB88_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055581.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1065..1856
                     /locus_tag="KPB88_003344"
     CDS             1065..1856
                     /locus_tag="KPB88_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055582.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1858..2553
                     /locus_tag="KPB88_003345"
     CDS             1858..2553
                     /locus_tag="KPB88_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055583.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2540..3271
                     /locus_tag="KPB88_003346"
     CDS             2540..3271
                     /locus_tag="KPB88_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055584.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3585..4385
                     /locus_tag="KPB88_003347"
     CDS             3585..4385
                     /locus_tag="KPB88_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0055585.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFAVKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4925..5641
                     /locus_tag="KPB88_003348"
     CDS             4925..5641
                     /locus_tag="KPB88_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055586.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2390 a          734 c          851 g         2132 t
ORIGIN      
        1 taaaaaatct acaataagtt aatttgattt aagtttttaa atttaaaata ctttaaagta
       61 ttaataaaaa ttaaaaataa aataactacc tatggaaatg acattataaa tagatttttt
      121 cttaactata cataacaaaa cataacaaaa cattataaaa cataacaaaa acttagacat
      181 cgttttctta gtatgatata ctaaaaaaga atttacacta atttactaag ggggaatatc
      241 ttatgtttaa aaaactttca gtactagctt tagctatgat tatttcatta agtattactg
      301 catgtaattc taaagaaaac aaagctaatg atgaatctaa tactaaagtg acaacagctg
      361 atgattcaaa agaaaaagag actctttttg tctattcagg ggcaggttta aagaaaccaa
      421 tggatgaaat agcaaaaaaa tttgagaaag aaaataatgt caaagttgaa tatagctatg
      481 caggttctgc acagctaata gctcaaattg agacaagtca caaaggagac gtatttattg
      541 ttggttctga accaatttat tcaaaggcct ttgaaaaaga acttgtaggt gaacataaaa
      601 cggttgcaca tcatacacct gcaatagttg tacctaaagg aaatccagct aatattcaaa
      661 aattggaaga cttaaataaa gatggagtta aacttatttt gggagacaaa gagtctaatg
      721 ctataggtaa gactacacaa aaaatacttg aaaaaaataa tttaaaatct ataaatgata
      781 atgttgtctc tacagctgca actgtaaatg aaatgattgt acagttaaca tcaagtaaag
      841 ctgatgctac aatagccaca aaagattcgg tctttggtaa tgaagatgta gaagttatag
      901 aaattgccca agataaaaat atagaccaac ttatatcagc aggaatcgta aactatagtg
      961 ataaaaaaga tttagctaat aaatttataa attatataga gtcagacgaa gctaaacaga
     1021 tatttaaaaa atatggtttt gaacctgtta agtagtgatt aaacttgaaa aataaatttt
     1081 ttactactct acttactgga ttttttgcta tagtcttttt gtttattgta tcaccacttc
     1141 ttatgttaat cattaaaggg ataagttatg ttcctatatg cttaaaatca gtggaagtac
     1201 agtatgcaat aacacttagt attaaaacat ctttaatatc tactatagta tgccttttat
     1261 tagctattcc agtagcatat tttttacata ttactaaatt accatttaaa aaactgatta
     1321 tacagataat aaatctccct atgtctcttc cacacttagt atcaggaatt gctcttttac
     1381 ttttgtttgg aagaatgggg attggagatt ccatttataa gatttttaag cttgatttta
     1441 tatttacaaa gcaaggtata gttttagcac aaatatttgt aaatcttcca cttactatta
     1501 aaatacttca cacttcatta aatgaaagta atgaaaagat gatatttgtt gcacgtaccc
     1561 ttggatgtaa tagttgggaa gcatttagat ttataattct tccaaatcta aaaactggaa
     1621 tcatatctgc aactgtaatg acttggtcaa gagcacttgg tgaatttggt gcagtagcta
     1681 tgatagcagg ttctacacgt atgaaaactg aaattattcc tacatctata tatttaaata
     1741 tgtctactgg tgatatagac attgctatcg gaatagctgt aattttgata tttctctcac
     1801 ttacatgctt gatgcttttt gagatatttt ttaatagaga ggttgataag aattaatatg
     1861 ttaaaaataa aaaatttatc tcttgattta cctaatttta aattacaaga aaatagttta
     1921 aatattaata atcatgaata ctttgtttta ttaggagcta gtgggtcagg taaaactcta
     1981 tttctagaaa cattagcagg aagatatcca tctgctaaag gaaatgtatc atacaaagaa
     2041 aatttaatat caaacttacc tccagaaaat agaaatatag gatttgtata tcaaaatttt
     2101 gaattatttc caaatatgaa tgttgaagat aatataaggt ttccacttaa attgagaaaa
     2161 atacctatag atgaacagat tcttaaggct aaccacttat ctaaattaat gcagatagaa
     2221 gatataaaaa ataggtatcc taaaaattta agtggaggcg aaaagcaacg agttgctttt
     2281 gcacgtgcct taatagctga acctgatata ttacttttag atgaacctat gagttcattg
     2341 gattatatta caaaaaaaca tataagcaaa attttgaagg atgtctacat aaaatacaaa
     2401 ccaacagtga ttcatgttac tcatgatatt tctgaagcaa tgttttttgc tcataagata
     2461 ggaataatga acaatggaaa aataaatcat atttttgatt taaatgaaga aataaaaaat
     2521 aaaggagaat cttttttcta tgaatatatt tgatattttg agagattatc tattagtaca
     2581 agtcgataaa tataatttaa atatggatat gataagtatt gtgtccaaat ctctatcttc
     2641 aaaagaagca attggaaata ctaagcgtaa agatttccca attatagttg gtaaagaaat
     2701 aatgcttgaa gctgacttta aaggagctaa aggacaagca tttacttcta ctccatctac
     2761 atttgaaggt tctttaaaag acatattatc attagattta catgacaatc ctcatgatag
     2821 aagtcttttt atagcatcat taaatgcagt tatgaaatac ttaggcaaga cggatagaac
     2881 cattcattgt aaaaataacg agccagaggt atgtgcaaaa aaattccctg agtttataaa
     2941 aatggaattt ggtaatccaa aagtagcaat tataggatat caaccagcca taattgataa
     3001 tattaaggac tttttcgaaa caagagtatt agatttaaat cctgaatttg tggatactat
     3061 tcaatataat gttaagattg aagatggtat acgagattat gaagatgtaa tatcttgggc
     3121 tgatttagtg atttgcacag gaagtacatt atgtaataac tcaattataa attttctatc
     3181 acttaacaaa ccagtttact attatggaac tacgatagct ggtgcatcaa atattttagg
     3241 tttaaaaaga ctctgttttt gtagtaaata agcaatcata tcatcaagga gtacacaaat
     3301 atatatgtgt actctttaaa tttaaaaagt tattactttt attctaagta ctaaagtaaa
     3361 attctattaa aatgaatccc gaataaatgt acaatccctt taaatctcaa ttcctatcaa
     3421 tcattaatat tttgattagt cttatacata aatgttttct ttattttaaa ctaataaaaa
     3481 aattttattc cattaatcta ctctatattt ttcagcattt aagaaataat ttttagtact
     3541 ttgtttgata atataactaa gacaactttt caggagatga ttaaatgaca tttaagaata
     3601 aaaaatttac tataaataaa tataaaattc tatctattat tctagtttta tgtttgattt
     3661 taaccgcttt tagtagctat gtgaattata gtcataaaaa atttaaagat tatgttgtct
     3721 tctatgttca gcatcaagat gatgaagtgt tgtgggctgg gagtgctatt gttaatgcta
     3781 taaaagaaag aggaaaaaat catgtatttg tggttttagt atctactgga tgtggtatat
     3841 ccgtctttga taaatataaa aaatatgaaa acctaacaaa tatacaaaaa tcagaatata
     3901 gaaatagaga atttttagct tctcttaata gccttggcgt aataagggaa aatataatat
     3961 tattaccaga aacaaataca aatggtaata ctgacttcaa ttacatggaa aaaattgctc
     4021 ttacatttga agaaaaattt aaaaatgtaa ctcatatagc acatacttat aagttagatg
     4081 accatttaca acatttaaaa aatggttctg ttatccaaaa tttatataat gctggtaaaa
     4141 taaaagatgc taaatatttt gtaaaaccta aatttgctgt taaaataact tttaataata
     4201 agatagttta taaagctcat tctactgaag actatgaaaa ggttaaaaat gcctgtcaac
     4261 aatacaaaat tgttgatgaa ttagaatata gagaaggtat tggctataag tctgaccata
     4321 aatcatttga taagttatta aataataaag atgttcctgc tatacttcat acacctaatt
     4381 tataagaaat atttattctt ttaatttaag ttataaaaag gaaattattt gagattttgt
     4441 gatatagcac tttttgtgct atactacttt ttctcataca actattaccc atatctaatt
     4501 tcatcatctc ttaaaatata taaagatatc atcattaaaa cctataagcc ttattaaatg
     4561 ctttatacat acctacatta aactacctgt ttatattaaa ctacattaaa ataaatattt
     4621 gttataaatt cttaataatt atgaaactca tttgttattt atatttgtta atatatgttt
     4681 aaacctaaaa aattaataaa ccaaaataac ataagaagtg tgatagatta ccctagatat
     4741 tttcataaac ttttaccttc agattaatat gggcgtctat acttttaaat ttactatatg
     4801 cattgacaat atattaaaag tactagtttt tatttcataa tgaatgggct tactgttttc
     4861 taatacctct ttttggtata aaataaaaat tggttaaaaa taaaatttca agggggttaa
     4921 taagttgaat caaaatatat tggttgtaga tgatgataga gagatagttg atgctataga
     4981 gttctatttg agacctgata actttaatgt tttaaaggca tatgatggac ttgaagcatt
     5041 ggacatacta atcgaaaatg atattaagtt aataataatg gatgtaatga tgcccaattt
     5101 agatgggctt aaaactacac ttaaaataag agaaaaaagg aatataccaa taattctctt
     5161 atctgcaaaa tcagaagata tggacaaaat tataggtctt aatatgggtg ctgatgacta
     5221 tataactaaa ccatttaatc cactagaact tactgctcga gtaaaatctc aacttagaag
     5281 gtatataaat ttaggtgatt ttagtaatac aaataactca aatgatacta attatattct
     5341 aaaaagtggt ggcctagagc taaatacaga tacaaaaata gtttctttag atggtgagga
     5401 aattaaaatg acaccacttg aatataaaat acttacactt cttatgtcac gaaaaggtaa
     5461 aattttctct tctcatgaga tttatgaaaa tgtctggaat gaggatgctt atagttgtga
     5521 gagaactatt gctgttcata ttagaagaat tagagaaaag attgaaatta atccaaaaga
     5581 accaaaatat ttaaaggtgg tgtggggcat tggttacaaa attgaaaaac ttaattatta
     5641 atgattctaa agcaaaattt attttattat tattactgtt tctattagtt tctatattat
     5701 ttgaagcagt ttatgatttg aataataact tttctaatat taattattat tatagagagt
     5761 ttgctctccc ttatttttat tttttcggta ctcctgtaca tttgtcaaaa gtagaattag
     5821 agtttgtctg cataggtatt atattatcaa gttttttatt gactatacta tgttttacat
     5881 tgtataaaaa aaataaacaa actaaagtca aattcttaga gaatgtcata agcctttttt
     5941 ctaaggtaaa aatagaactt acacttatat taactttgat actattaacc ttaaggtttc
     6001 atgcatttac aagtaataaa atgttttaca acactaattt aataatctac aattttataa
     6061 ttatatctat agtctatata atatatgaaa atattaaata taataac
//
LOCUS       DAELJO010000208         6064 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELJO010000208 DAELJO010000000
VERSION     DAELJO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.210"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..2212)
                     /locus_tag="KPB88_003349"
     CDS             complement(<1..2212)
                     /locus_tag="KPB88_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0055587.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENN"
     gene            2530..5958
                     /gene="uvrA"
                     /locus_tag="KPB88_003350"
     CDS             2530..5958
                     /gene="uvrA"
                     /locus_tag="KPB88_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0055588.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSNEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARIRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNLLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
BASE COUNT         2254 a          893 c          846 g         2071 t
ORIGIN      
        1 tattattttc atatgcaaaa ttttcaaagt cagaaattaa taatggtttt gaaaaaacat
       61 aaccttgaat ataatcacat cccacatttt ttagaaactc aacttgttct aattcttcaa
      121 ttccttcagc agttactttc atacctagcc ttttgctaag ttcaataata gacttaataa
      181 ctaccttacc acggtctata ttatttttgc taacaaagaa tgaacggtct aacttaatta
      241 tatcaacttt aaaatccttt aatattccta atgatgagta cccaaatcca aaatcgtcta
      301 tagaacactt aaatccatta gaatgaatat cctctattat atctatagct tctggatttt
      361 ctaagaaaat agattcagta agttctaaat ctatcagaca tgttggtaca ttgtatttat
      421 tgcatatatg cttatatttt tctaaaaaca atttatcatt cagatgttga cgtgatacat
      481 taacagaaat tctcaacact tctttacctt cttttatcca cttagaaatc aatttgcatg
      541 tttcttctag tatatataaa tctaacttac atataaatcc acttttttcg aaaagaggaa
      601 taaaatcact tggataaatt atgccctttt ctgaattttt ccaacgcaca agagactctg
      661 ctccactaat ttttccactt tttatatcaa ttttgggttg taagcacata aaaaattctt
      721 tattttcaag ggctttttcc attaaatttg aaatttcctt ttcagatatt aatcgttttc
      781 tttctatatc actataaaaa gaaaaatcac tttgatgagt tttattaaag ctttttcttg
      841 ctgtatttgc atggtcttgt attgtaatta aatcttcatt atgattttca attgtgtaaa
      901 tacctactga tagtcttaaa aagtatggat tttccctatt tttattaaaa taatttgtgt
      961 cagcttctat tttatataat aactccaggg cttctaacac tggtctattc ttaataagca
     1021 aatagaaaag gtctgcatca tgacggcaca caaattcttc attttcatca atgtttttat
     1081 acaaaatatt gtaaagatgt tttagtgtca tatttccctt ttcactaccg aaacaatcat
     1141 ttattagctt aaaatctttt atattcattg ctatcatcat ataagtacta tcaggtgatt
     1201 ctgaaattaa aggtttgact agcatactaa acttagttga atttatacca tttgttatac
     1261 tatctaaata tgcaatattt tctatttgat ttacatattt tcttcttata atatataaaa
     1321 ttaatgttat aattccaata atacatgtaa ttataaatgt cattgttatt gtaaaagagt
     1381 ttaatttatt aagttcacca agtacagcat cttcaggtac tatgagaaga aaatgccaat
     1441 catttaaacc tcttgtaatt ggatgataag ttaataagca ttgtacacct tttgatgagg
     1501 ttattgtaat attccctgat ttatcatttt taaaatcctc aagcatcttt tgtgcccaag
     1561 gttcatctcc agaactctta tatttatggc taaggtcctt tattaattca gtattttttc
     1621 ctatgaaaag aggttttccc ttactattaa taatttcaat agttcctttt ccattaaagt
     1681 tatctatatt aagaatctca ttcatacttt ctttattgga tacacctatt aaaatactag
     1741 atacttcatg attatcatta aatataggta cactataaag aacaaaaccc attgactcta
     1801 ttacttctga gtattcttta ccttcaattc cttttttata ggaaacagta ttctttaagt
     1861 caaacttttt tccacttaaa aactcagctt taccttctaa atctattatt ccaacatcat
     1921 aatacttata actagtcttt cttttagata aatactcata tcgactatca ccttcatatt
     1981 gcttaataaa atctgccatt attcttaagt cggataaaat aaatttgatt ttagaatcaa
     2041 cattcattac aatctgtgta gatacatcat ttaaatagtt tttagattgt ttattagata
     2101 tattgtgtag ttttacggta ttaaaaacag taattacaag gattgaaata ataagtgaac
     2161 ataaaattat agacagaact aaactatttt tcttatacct tgataatttc atttttttcc
     2221 tccaaattat aaacctattt tttaatgtat attaacactt acaaattttt ttactaaaaa
     2281 tccttcacac tatattatag tattctattt cttaatattc aagaatgcca tcttaacatt
     2341 tcattaacat tcaatagata atggttttat taaataatta tattatatgc aatttgaaac
     2401 tatatttgta ttacaattta tcactgtatc attttttatt tattttaaat tttggccaaa
     2461 aaatctaaat tgtccttgca tatgacacca tgtcagggta tatcatccat ttagagaggt
     2521 gaaatataaa tgaaaataaa taactataaa aatcaaagta tcatcacaaa tcctaaaaaa
     2581 tttgaaaata aatatcaaga tttaccaaag actccaattg agttattaaa agtggttcaa
     2641 agcctagtaa ttcatggaga ccaagggaaa ttatatggta tatcctttaa taagcaacaa
     2701 tctaatgagg aattgcttcg tacaattcct caaatgttaa aaagaatatt tgaaataaat
     2761 tctaacccac taactattcc aagaaaccca aaacaaaggc ttgttggaat gtgcagagat
     2821 tattctttac ttcttgtatc gctcctaaga tatcgaggtt ttgaggctag aataagagca
     2881 ggttttgcta attattttga aagtgagctt acttatgaag accactggct tgtagaatac
     2941 tatgatactc taacaaagag atggataaga atagatgcac aaatagatga catacaaaaa
     3001 aattattttc aaattaattt tgacactcat gatgtcggaa aaacagatgg ttttttaact
     3061 ggttcagaag cctggataag atgtcgtcaa ggacatgctc atccagatga ctttggatat
     3121 aataaaaatt ggaaaggttg gcattctgta aagggtaatc ttctacatga ctttaataat
     3181 atgattggtt tagaattgct tccatgggat ttatggacag aattaagttc caaaaaatac
     3241 aatctattaa caagagcaga aaaaaattta ttagacgaaa tggcagaaat attatcaagc
     3301 ggaaatataa aaatagaaga ccttaatttg ttaatagaaa aattgcctga agattatttg
     3361 aaatcaatct tttctcagtt aaaaatatta ggcatttcgg aaataaaaga attaggcaat
     3421 cctcttgaac tcgaaaagaa attcaaattt acaaaatcaa taaataaatc aattaaaaat
     3481 tcactttgtc ataataaaag ctctatctat ttaaaaggag gaaggcaaaa caatttaaag
     3541 gatgttgagg ttacaattcc aaaaaatcaa ataactgtta ttacaggtgt aagtggtagt
     3601 ggaaaatctt ctttagcttt cgatacgatt tatgaagaag gaaaaagacg ttattttgaa
     3661 aatctaagta atggagcaaa actttcagaa cagcttcaaa aaccggaatt tgatttactt
     3721 cagggattaa cccctactat agctatcgaa cagaaaaaag gaagccaaaa tcctcgctct
     3781 actgttggta ctcttacaag catttgggat tatttaagaa tgttatttgt atcaatcgga
     3841 aaaagttact gtccatattg taaaattcca ttagaaaaaa agaataatac aaaaaattat
     3901 tgtccacatt gtcaaactat tttttctaag ataaatactt caaccttcaa tgctaactcc
     3961 catactggag cttgtcacga ttgtaatgga ttaggtttta cctatcaagt taatcctcaa
     4021 ttgattgtaa aagacccaac tatttcaata ttagatggag ctacttacta ttttggaaaa
     4081 ttgaggggga aaaagccaaa tggtaattgg atggtaggtg aattatatgc aatagcaaaa
     4141 gataaaaata tagacctaga tataccttgg aatgaattac caagagattt tatagatgca
     4201 atcttatatg gtacagacga taagatatat gaatttagct ttgaatcaaa gggacgagaa
     4261 tcaaagatta gaagacctgc ttcaggagca atcaaccaca ttcaaagact ttttagagaa
     4321 tctagttctg aaaataatac cttgcatcaa tatatgaaca aaattccttg caatacatgt
     4381 ggtggcgaat tgctgtgcat tgaggctaga ttcactacaa tcaaaggtta tcgcttccct
     4441 gaactaacaa aaatgacgat agaacagtta tggaattggt tgtgtgaatt accaaatcaa
     4501 cttcaaaaaa atgagctctc tttggtaaat gatatcttaa cagaattaaa aattcgagtt
     4561 agttatctat taaaggtagg tcttagctat atatcaacag atagaacagc tccaacttta
     4621 tctggaggag aactgcagag agtacgatta tcaagtcagc taggaagtga attagtaggc
     4681 ttaacctaca ttttagatga accatctatt ggtttacacc caagagacca taatttaatc
     4741 atcaaaatga tagaagaact aagagataag ggtaatactg ttatagttgt ggaacacgat
     4801 aaagatacaa tactgtctgc tgattatatt attgatgtag gcccaagtgc tggtacaaaa
     4861 ggggggttca ttattgcaga aggtacaacc caagaaatta taaaaaatcc aaactctata
     4921 acaggtaaat atttaagcac atacaataaa ataagttcac aaaataaaac tataccaagt
     4981 aaatggttaa gcttaaaagg atgccatgca aataacttaa aaaatattga tgtagaaatt
     5041 cccttaaact gtatgtgctc tattacaggt gttagtggtt ctggtaagag ctctcttgta
     5101 tttcacagtt tacttcctgc tctagaagaa aaattgaaac aaaaatctat cccagataaa
     5161 aattatactg aatttactgg atttgatgct attgatgatt ttatcttaat ggaccaaaca
     5221 cctattggaa aatctagccg ttctacacct gctacatata tcaatatttt tgatgaaatt
     5281 cgttctctat ttgctgaaac tccacaagca aaacaaaaat tattggatga aagctatttt
     5341 agctttaata gcaaaaaggg gcaatgccca aactgtcaag gattaggtaa aacaaaaatt
     5401 attctgcaat atatggcaga ccaatgggta acttgtagtg aatgtcaagg taagagatat
     5461 caaaaagaaa tactgtctat acaatacaag ggaaagacaa ttgcagatat acttgacatg
     5521 gaagtagcag aagcaaaaac attttttagc gattgctctg atatatatag aaaactctcc
     5581 ttattagatg aagtgggact tggatatcta aagcttggac aaaatacatt aggattaagt
     5641 ggaggagaat ctcaaaggat aaaattagca aaagaattag ggacaaaaac taaaaaacga
     5701 atgctgtaca tattggatga gcctactact ggacttcatt ttaaagatat agaaaatttg
     5761 ttaattactt ttagaaaact tgtaaatgaa caacattcac ttttaattat tgaacacaat
     5821 acagaggtta tacgtgcttc tgattggatt atcgatatag gtccagacag tggaataaat
     5881 ggaggggaaa ttgttgcttc tggaactcct gatgaaataa aaataaatcc aaatagtatt
     5941 actggacatt ttatataaaa acaattaaag acacatgaac ttagatataa aattatagaa
     6001 tatcttatat tatagaaaat ttattataat agctatataa taaaaaaatt aactttagta
     6061 tatt
//
LOCUS       DAELJO010000209         6041 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAELJO010000209 DAELJO010000000
VERSION     DAELJO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.211"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            19..1332
                     /gene="aroA"
                     /locus_tag="KPB88_003351"
     CDS             19..1332
                     /gene="aroA"
                     /locus_tag="KPB88_003351"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG0055589.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            1310..2389
                     /gene="aroC"
                     /locus_tag="KPB88_003352"
     CDS             1310..2389
                     /gene="aroC"
                     /locus_tag="KPB88_003352"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG0055590.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            2392..3588
                     /gene="pheA"
                     /locus_tag="KPB88_003353"
     CDS             2392..3588
                     /gene="pheA"
                     /locus_tag="KPB88_003353"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG0055591.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            3635..4444
                     /gene="aroE"
                     /locus_tag="KPB88_003354"
     CDS             3635..4444
                     /gene="aroE"
                     /locus_tag="KPB88_003354"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0055592.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            4456..4989
                     /locus_tag="KPB88_003355"
     CDS             4456..4989
                     /locus_tag="KPB88_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG0055593.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            5010..5849
                     /locus_tag="KPB88_003356"
     CDS             5010..5849
                     /locus_tag="KPB88_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG0055594.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         2493 a          596 c         1077 g         1875 t
ORIGIN      
        1 ttttcaggag gtaagataat gggaagtcta aaaatatatc caagtaaatt atctggagat
       61 gtaaaaatac caccatcaaa aagtatggca catagggcag ttatttgttc atctctaagc
      121 aatggaaaaa gtagaatttc taatatagat ttttctgatg atataatagc tactataaga
      181 gcaatgacat cattaggagc tattatagaa aagaaagaag atattttaga aatttcggga
      241 attttttcta aagaaggtat tttaaataga gagaatcagt tgaatcaacc taaattgaca
      301 atagattgta atgaatcagg ctctacattg agatttttag tacctatatc tttaacattt
      361 gatggtgtaa aaaggtttat aggtagaggt aatcttggaa aaagaccact tgatacctat
      421 tatgagatat ttgacagaca gaatataaaa tatagttaca aagaaaatca gttagatttg
      481 attatatcag gtaagcttaa accagatgaa tttagagtaa aaggaaatat aagttctcag
      541 tttatcacag gtttactgtt tatattgcct acgctagaaa gtgattcaaa gataataatt
      601 actactgagc tagaatctaa aggatattta gatttaactc tttcaacgat aaaagatttt
      661 ggggtagaaa ttataaacaa taactataaa gaatttatta taaaaggaaa tcaaacgtat
      721 aaagcgagag attataaagt agaaggagat tattctcaag gagcatttta tttatcagca
      781 gatgctatag gagaagatat tagcatatta gaccttaaag aagattctct tcaaggggat
      841 agtgaagtag ttgaaatttt atctaggatg ggaatggaaa tattaagaga aggcaacaaa
      901 ataaaaggaa taacaaatgg tttaaatggt actttgatag atgcatctca atgcccagat
      961 ataatacctg tattatctgt agtagcatcc ttaagtatag ggaggactac aataattaat
     1021 gctggtaggc ttagaattaa agaatgtgat agacttcatg caataaatgt tgagttaagt
     1081 aaacttggag caaatataga ggagaaagag gatagtttga taatcgaagg agtaagcaag
     1141 ttaaatggtg gagtagaggt gtggagtcac aaagaccata gaattgctat gacacttgca
     1201 attgcttctt gtagatgtga taaaccaata atattaaaag attttgagtg tgtatcaaaa
     1261 tcttatccac atttttttaa agattttaag atgttaggag gtcgaattga tgagtggaat
     1321 atggggaaat aatttaaaag tatcaatttt tggagaatct catggaaatg ctataggtat
     1381 aaatatagat ggtcttcctt cggggattga acttgacctt gataatattg ataaagaaat
     1441 gaagagaaga gcacctggaa aaaattctat atctacttca agaaatgaaa gtgatatacc
     1501 agaaattcta agtggatatt ttaatggaag aactacagga acgccattat gtgcaatcat
     1561 aagaaatagt gatactcgtt caaaagatta tggagagctt aaaaatttaa tgagaccagg
     1621 acatgcagat ttcacaggta atgtgagata ttctggattt aatgactata gagggggagg
     1681 tcatttttca ggtaggataa cagctccatt agtattttgt ggggctatat gtaaacaaat
     1741 tttatcacaa aaaggaatag agataggagc acacataaaa aaaattaaaa atatagaaga
     1801 tatgagtttt gattatgtaa atatttcaaa acaacagtta tcaaatttgc aaactttgga
     1861 actaccttta cttgatttat caaaggaaga agccatgaaa aatacgatta tagatgcaaa
     1921 aaatcaagga gattcagttg gtggtataat tgagtgtgca gtagtaggta ttaatgtagg
     1981 gcttggaaat ccattttttg actctgttga atctacttta tcacatttat tgttttctgt
     2041 tcctgctgta aaaggtgtag agtttggttt aggttttgaa cttgcagata tgtatgggtc
     2101 tcaaagtaat gatgaaatgt attatgaagg aaatcaagtt aagagcaaaa caaataacaa
     2161 tggaggaatt ataggaggga ttactactgg aatgccaata attttcaaag tggcaattaa
     2221 gccaactcca tcaattagca gacaacaaaa cacagttaat ataaaagata aaaaggacga
     2281 tattttatat attaaaggca gacatgaccc ttgtatagtt caaagggcta tacctgtaat
     2341 agaagcagta acagccattg gaatttttga tttgatgaaa ggacgataat aatggaggat
     2401 ttaacgagat atagaaatga gattgacaat atagataaag agcttattca attatttgaa
     2461 aagagaatga atacagttct tgaaattgcc agacataaaa tgaaaaataa cactactata
     2521 cttcaaaaag atagagaaga aaaagtacta agtagagccg tagataattt agataataaa
     2581 gcatattcac aagaaacggt acagtttttt aattctataa tggaaattag tagaggatta
     2641 caaaaaagat tgatagataa tgatggtgag cagaaaatcg agtttagttt agattcatca
     2701 aataagaaat ttgatttgtc aaatgtaaat agatataagt ctttaaaaga tgaattaaat
     2761 aaaaaaaata tcctagtagg gtttccagga aaatcaggtt catttacaga agaagcacta
     2821 aataaattct tcaacaaaaa aatatcaaaa aaacaattta aagaatttga agatgttttc
     2881 atagcactta aaaataaaga aataaactat ggaataatcc ccatagaaaa ttcatctaca
     2941 ggagctatct ctgaaactta tgaccttttg agaaagtacg gtttttatat agttggagaa
     3001 gaatgtataa aaatagacca aaatttaata gggataaaag acacaaagct tgaagatata
     3061 aaagaaatat attctcatcc acaaggtata ggacaatgta gtgaattttt aaaacaaaat
     3121 agtacatgga aattgatacc atttaataac acagcaacaa gtgcagaact tgtaagaaaa
     3181 cttcaagata aaacaaaagc agcaattgca agcaaaaaag ctgcaagtat ttatggatta
     3241 gaaataataa gtccttgtat aaatgatatt gcaaacaact atacaagatt tgtagtaatc
     3301 agtaatcaaa tacacattga agaagaatca aacaaaatga gtgtagtatt ttctgttgaa
     3361 catgaagcag gaaaacttta taaagtactt ggatactttg ctaaaaataa tataaatatg
     3421 acaaaaatag aatcaagacc tatgaaaaat gcctcatgga gatatttctt ttatatagat
     3481 tttgagtgtt ctatatacaa tgctaaagtg tacgatttat tagaattgat agaacataat
     3541 acagcatatt ttaaatttat gggagtatat aaaaataaaa ctgaatagat aagataaatt
     3601 tttatttaat ttataattat taggaggtgt aagtatgaat tttttcggtc tagtaggtga
     3661 aaaactctct cacagtgttt cacctcaaat ccacaagaga gtattcgaga tattgaatat
     3721 tgaaagtgct tataaaaact ttgaaatttc aaaagaagat atatctaagc tagacggtgc
     3781 tataaaatta ttaggaatac aaggggtaaa tgttactgta ccatataagg agcgtattat
     3841 gaaatatctt gattttatat caccagaggc aaaacgtata ggagcagtta atacaatttt
     3901 acttagagaa aatatgttat atggttacaa tacagattac tttggtcttg atagtatgtt
     3961 taaaatggct aatattgatg ttcaaggaaa agtagcagtt atattaggaa ctggaggtgc
     4021 ttctaaagct gctcttacat attttattga ttctggtata gaaaagcttt atgtttcaac
     4081 tagaaaaaag gatgataaaa aattattaaa tagtaaagct atattaatag attatgaaga
     4141 acttaaacat ataaaaggag atataatatt aaatgctaca ccagtaggta tgtaccctaa
     4201 tgtaggaatt tcaccagtgt caaaatctat tatacaaaat tttgatatat taatagattt
     4261 aatttacaat cctggagaaa cagagttttt aagaattggt aatagtatgg gtaaaaaaac
     4321 ttgtgatgga ttatatatgc tggtggggca agcaataaaa tctcaggaaa tttggcaaga
     4381 tactaaaata gataatagta ttttagatgt tatatataat gaactaaaat tagaattttt
     4441 gtaaaggaga ttgttatgat aaataaaaca aaagaaaaat tgatattaat aggtatgcca
     4501 ggtagtggaa aaactacaat tggtaaactt ttggccaaag aatataattg tagtttctgt
     4561 gatatggatg attatataat tcaaatatct caaaaaagta tagcagagtt gttttcagaa
     4621 ggagaagata tctttaggaa ctatgagact caagcatgta gagaactttc tataagtgat
     4681 aaaacagtga tatctacagg tggaggtgta ataaaaaagg atattaatat ggaaatacta
     4741 aaagaaacag gtataataat ttttattgat agacctatac aaaagatttt ggaagatata
     4801 aatattaatt caagaccatt attaaaaaac ggaaaagata gattgtataa tctttacaat
     4861 gagagaataa atctttataa aaaattttca gatatagaga ttttaaatga taaatcatta
     4921 aataatgctg tatataatat tacaaatgca gtcagtgaaa attttaaatt tgactttaaa
     4981 gaaaaataat ctagggggaa ataaaaataa tgaatattgt tattgtagga ctaggagtta
     5041 taggaggctc ttttgctaaa gcattaaaaa aagctggata tgaaaatgta tttggagtag
     5101 atgtagattt agaaacttta aaaaaagcag aaaaagcaaa aatcataaaa aaaggatgta
     5161 ctacaggtaa agagttattt aaaaaagctg atttaattat actatctata tatcctagat
     5221 tagttgtaga ttttctaaat aataataaaa acttttttaa aaaagggact ataattactg
     5281 atactacagg aataaaagaa acacttatca atgatgtgtt gcaaattatt ccagatgata
     5341 tagattttat atttggacat ccaatggcag gtagggaaaa aaagggtatt gattttgcaa
     5401 gtgaacaagt ttttaatggt gcaaattata taattactcc aactggcaga aacaacataa
     5461 aaaatttaga gttagttgaa aatttaattt tagagattgg atttaaaaga gtaaaaaagt
     5521 taacttcaca aaaacatgat gagattatag cttttacttc tcagttacct catgttatgg
     5581 cagttgctct tataaatagt gatgaagaag gtagagatac gggtaaattt attggagata
     5641 gttatagaga tttaactaga attgcaaata tgaatgaaga cttatggagt gaactttttt
     5701 taggaaatag agataattta ctaaaagtta tagaaaactt tgaatcagaa gtaaatttag
     5761 taaaagaagc tatatttaac aatgataaaa ataaattaat agagtatttt aaaaaatcat
     5821 caataagaag agaagcttta gaaaaataaa tattaatatt taagctatat atagatgggt
     5881 ataaatataa gcgatagtgt atttaattgt attaataaat atggattcaa tataattaaa
     5941 tatgctattg atatatttgt acccttaact attgttatct tttaaatgat aatgatattt
     6001 atttaaatac tctttattaa caatttataa aacttaattg t
//
LOCUS       DAELJO010000210         5982 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELJO010000210 DAELJO010000000
VERSION     DAELJO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.212"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(73..2667)
                     /gene="clpB"
                     /locus_tag="KPB88_003357"
     CDS             complement(73..2667)
                     /gene="clpB"
                     /locus_tag="KPB88_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG0055595.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2704..3300)
                     /locus_tag="KPB88_003358"
     CDS             complement(2704..3300)
                     /locus_tag="KPB88_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055596.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3317..3721)
                     /locus_tag="KPB88_003359"
     CDS             complement(3317..3721)
                     /locus_tag="KPB88_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG0055597.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4088..4462
                     /locus_tag="KPB88_003360"
     CDS             4088..4462
                     /locus_tag="KPB88_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055598.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4459..5322
                     /locus_tag="KPB88_003361"
     CDS             4459..5322
                     /locus_tag="KPB88_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055599.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5322..5960
                     /locus_tag="KPB88_003362"
     CDS             5322..5960
                     /locus_tag="KPB88_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0055600.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         1974 a          919 c          689 g         2400 t
ORIGIN      
        1 ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata ttatgctcaa
       61 ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat ctataactat
      121 agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag tttctaatgt
      181 atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc cttctctcac
      241 cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt ttaatctatt
      301 ttttaaatct ttcataaata tatctataat tttcttgata ttttcttggt ctaaaggctt
      361 aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat gcttcatttc
      421 attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta atagataatt
      481 acttcctata ttagaagtca ttattataag agtattttta aagtctactg tcttaccttt
      541 attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata catcctcatg
      601 tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa ctgcttctgt
      661 aagttgacca ccctcttcat accccacgta ccctggagga ggtccaacta gtcttgatac
      721 agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt cactatcgaa
      781 taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag ggcctaagaa
      841 aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac gtattactgc
      901 atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa gttcatcttc
      961 tagtttaagt aatttttctc tttctccttc aactagttta gttactggta tacctgtcca
     1021 ctttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa gagaattttc
     1081 agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact taggtatttc
     1141 accatattta acctctgctg ctttgttgaa atcatattct ctttcatact tttcagcttt
     1201 acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt gactcttttc
     1261 tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta tttctttttg
     1321 aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg cttctctttc
     1381 agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca atgaatctat
     1441 ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat ctggtaaaaa
     1501 tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat tatcatgtat
     1561 tcttactcca tgatgtatct caaatctttc ttttaaacct ctaagtatag atattgtatc
     1621 ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag ccttatcttt
     1681 ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca attcacctct
     1741 tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt taccagctcc
     1801 aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac tttgaacttc
     1861 ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac cagcaattaa
     1921 agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga catctcctct
     1981 tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg gctcaccaat
     2041 caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc ttatttcttc
     2101 atctctccca attacagggt ctaatttatt tttcttggct aaatctacta aatttgttcc
     2161 atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc tttgactacc
     2221 tctcacttgt gataaaatat ttaaaaagtc attcttattt atattatatt gttttaatat
     2281 tttaccaaca gctgattttg attctgtttc catcattgca agcataacat gctctacact
     2341 tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca cctcatttat
     2401 ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatactt ttggaagctt
     2461 atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca ttttttcgat
     2521 tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat gaattacatc
     2581 tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac ttttttgaac
     2641 tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt atatccaaat
     2701 aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac tatcaagtaa
     2761 attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat ctgaattaaa
     2821 aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta taagtctttt
     2881 ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat caatagtgta
     2941 tagattttct aaaatattat attgataaat agcaatatta ttaaacactt ttaatatgtt
     3001 tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt cgtatatata
     3061 ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag ctggtatttc
     3121 aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta ctttgttcaa
     3181 actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca aactagtttt
     3241 ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt ttaaactcat
     3301 ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga ttatattctg
     3361 ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga tttatcatat
     3421 actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta aacaatgaag
     3481 ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt tttctaatct
     3541 tattattttt atccatagag tttattaatt ttaatggaat taaatccctc ttagtataca
     3601 ttccttctac tactttaata taattcttaa aacaaacatt catatactta cttttattca
     3661 taattaaact tttgctctct gtatattcct gatttaaatc cttttttaat gtcttatcca
     3721 aattaaatat ctcttctcac tctccttatc tctaattgat aatataaata tattacaaaa
     3781 aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa cttttattta
     3841 gctataagct aaactttatt atttttttaa tttagtattt ccaatttatt ggcaggattt
     3901 ttgaacaata tttataaatt ttttaaaaaa atttaaatct atttaatatg gcagactttc
     3961 ataaaataca ttacatttat taaatgtata atattggtaa ttagtttttt ctaaaatcag
     4021 ttgacatatt atatatatat ttatataatg tgtatatact atatatacaa tttaaggagg
     4081 tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg aacagattac
     4141 tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag ctcttccaag
     4201 tatgagacta ttggcaaaag aacttagaat aagtgtaatt actacaaaac gagcatatag
     4261 cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt ttgtatctag
     4321 taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat acctacaaaa
     4381 agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag atattttatt
     4441 tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa tttaaataaa
     4501 acttataaag attttagcct acaagatata tctttttcag taccaaaagg gagtgtaatg
     4561 ggatttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat tttaaattta
     4621 atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa aaatgaaaaa
     4681 gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga aaatttaaac
     4741 actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga cacattattt
     4801 aaagactact taaaaaaatt gagagtacca gataataaat taattaagga gttttcaaag
     4861 gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa attattaata
     4921 ttagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact agatatattt
     4981 ttagatttta tacaagatga agaacactct attatcttat cttcacatat tacaagtgat
     5041 ttagataaga tagcagacta tattacattt atacataaag gaaaaattgt atttagtgaa
     5101 aataaggatg aattaattga tactatggga gttttaaaat gtaaacctag tgattttgat
     5161 aacctttcaa gagaggatta ttcatattat cgtaaaagcc aatttggata tgaagtactt
     5221 cttaaagata agcatagatt tatctcaaga catccaaact gtattgttga taatacgtct
     5281 atagaagaaa ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt ctaattttaa
     5341 aagatttact taatttgaaa ggaaatataa agtttatact tttattcatc attatgtttg
     5401 gctttatgtc ttctttagga gatggaaatg ttaataactt tataggtgtt attatagttt
     5461 tatgtactac aatgattgtt tcaacatttt catatgatga tttgaataaa tgggatagtt
     5521 atgtacttac aatgcctatt aatcgcaatg atattgtatt aagtaaatat ttaactatgt
     5581 taatatttag ctttataggt gttcttgttt cacttattgt tagtgtaact attggttatt
     5641 ttaaaaatac acttatatta aatgaaacac tacttattaa tgcactaatc ttatcaattt
     5701 cagtatgttt tggtagcctt attctaccac ttatttataa atttggtaca gaaagagctc
     5761 gtttattaat gattttatgc tttttagttc caacactagc tttattagtt tttaaaagta
     5821 ttttagaaaa tattagttca cctatttcta tagaaattat acttaataca ctagtatatt
     5881 ctcttccatt tgttgctatt ttactatttg tgatttcata ttttatatca tcaaaaatct
     5941 atagtaaaaa agaagtataa aatatactat atatgtttct gt
//
LOCUS       DAELJO010000211         5954 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.213, whole genome shotgun sequence.
ACCESSION   DAELJO010000211 DAELJO010000000
VERSION     DAELJO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.213"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..799
                     /locus_tag="KPB88_003363"
     CDS             <1..799
                     /locus_tag="KPB88_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0055601.1"
                     /translation="NRFSYKTNFTNRNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNA
                     TISKEPSDRYYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKS
                     LNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIIC
                     IEDLQVKNMIRNHKLSRLISDVSWSEFIRQLKYKANWHGRKIVKVGKFFASSQICNKC
                     GYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            917..1606
                     /locus_tag="KPB88_003364"
     CDS             917..1606
                     /locus_tag="KPB88_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0055602.1"
                     /translation="MNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRD
                     YIQEEILGLKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQV
                     MAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDP
                     PRPGIHKDAIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTP
                     HCETVVKLIRE"
     gene            2129..3163
                     /locus_tag="KPB88_003365"
     CDS             2129..3163
                     /locus_tag="KPB88_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG0055603.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGSCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDIGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNNYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            3250..3453
                     /locus_tag="KPB88_003366"
     CDS             3250..3453
                     /locus_tag="KPB88_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0055604.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            3624..4784
                     /locus_tag="KPB88_003367"
     CDS             3624..4784
                     /locus_tag="KPB88_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG0055605.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNKNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            5044..5184
                     /locus_tag="KPB88_003368"
     CDS             5044..5184
                     /locus_tag="KPB88_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055606.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         2403 a          590 c         1046 g         1915 t
ORIGIN      
        1 taatagattt tcatataaaa ctaactttac aaatagaaac attatgtatt gtggtcaaca
       61 tataaaacta cctaaacttg gtatggttaa gataagggat aaacaagtac ctaaaggtag
      121 aatacttaat gctactatat ctaaagaacc aagtgataga tattatgtat cattatgctg
      181 tactgatgta gatattgaag catttgaaaa tactaataat cagataggtt tagatttagg
      241 aattaaagaa ttttgtatct caagctgtgg agaatttata gaaaatccta agtatcttaa
      301 gaagtcatta aacaaacttg ctaaattaca aagagaatta tcgagaaaga caatcggtag
      361 tttaaatagg aataaagcaa gattaaaagt tgcaagactt caagaacata tagctaatca
      421 aagaaatgat tttttacaaa aactatctac taaattaata aaagagaacg atattatttg
      481 catagaagat ttacaagtaa agaatatgat taggaatcat aaactatctc gcttaatttc
      541 tgatgtatca tggtcagaat ttattcgtca attaaaatac aaggctaatt ggcatggtag
      601 aaaaattgta aaggtaggta aattctttgc aagttcacaa atatgtaata aatgtgggta
      661 taaaaacgag gaagttaagg acttaaatat aagagaatgg atttgtccta gttgtaatga
      721 aactcacgat agagatataa atgcaagtat aaatatacta aaagaaggat taagactaat
      781 aacaattcaa aataaataac ttatgtaaga accgtaggaa ctacggggat ggcctgtgga
      841 gaattagtaa gacatatttt agtatgcaaa attctatgaa gcaggaaccc tgtgacttta
      901 gtcatgggag gttcagatga atgagtttaa agatatgctt ttagggattg atttaaaagc
      961 tgagttagta ggtgtacttc atactattaa tgatggttta gcagatgcag tccaatgtga
     1021 tgagttaaga gtattgtatg gaagagatta tatacaagaa gaaattttag gtcttaagtt
     1081 taagatttca ccattttcgt ttttccaaac caatactaag ggagcagagg ttctttatag
     1141 tattgctagg gattttattg gagattacaa tgataaagtt gtatttgact tgtatagtgg
     1201 tactgggtct attggacagg ttatggctgg tgctgctaag aaagtgtatg gtattgagat
     1261 agttgaggaa gctgttgttg ctgctaatga gaatgctaag ttgaatggac ttactaattg
     1321 tgagtttatt gctggggatg tggctaaggt tgttaaggat ttaaaggaca agcctgattt
     1381 gattattgtt gaccctccta gaccaggcat tcataaggat gctattaggg atatttgtgg
     1441 atttggtgct aaggagattg tttatatttc ttgtaatcct aagtctttgg ttgttgattt
     1501 ggttgatttt aaggggtatg gttatgagat taagatggtt aagtgtatgg atatgttccc
     1561 aaatacgcca cattgtgaga ctgtagttaa acttattaga gaataacagg gtttcagact
     1621 tttatattgt gtcttaaaag tagatttgcc acctatttgc caccataatt atttttgggt
     1681 ggcaaattta ttctacaatg attttaaaaa tatttaccgt ctggtttctc atgttattag
     1741 ttacatgtaa atatgtatcc atagttgttg aagttttagc atgtccgagt atatgctgga
     1801 tatcttttat attagtacca ttttctaata acatagtatc agggttttta aaataaagtg
     1861 tgtattcttc taaagtagtg ataattaaaa tatcaaatat cactatacaa aggaaagtgc
     1921 acgcttttta tgtttaacaa aaacgaaata ttaaaaaaac ttattgaaga atatgatgtt
     1981 aaaacaacta cagatattca atatatgcta aaagatttat ttacaaatac aattcatcaa
     2041 atatttacat tgtcgaattt taacggttta aacataatat attgactgat ataataaaat
     2101 catagaatat tttaggggga aatagattat gtttaagaaa aagttaatag ctgttgcaat
     2161 gagtataact atgattagtg tagggagctt ttcgtatgct cattcaggaa gaactgattc
     2221 atcaggaggt catcgtgata ataaaaataa atctggatta ggaagttatc actatcattg
     2281 tggtggttat ccagctcatt tacataataa tggtagttgt ccttatactg gaggaggaag
     2341 cagttctgga acaacaactt cggttaataa tgaagaaaag caaaagaggt ctgttggaga
     2401 aaaaggatat aatcaagggt atgaagatgg gtataaagga aattatagtt catcaaatta
     2461 tagtggagat tatagtgata catatgaatc aaagtactca gaaggatatg aaaaaggtaa
     2521 ggctaaatta gaagaggaag aaaaggtggc taaagagact ggatataatt tagggcttac
     2581 aggtgctaaa tctaataata catatgaaaa agaagtttta aaaaatgctt atgatatcgg
     2641 atattcaact ggatacaatg aatataagac taaaaagata gaagaatata aagctaaagg
     2701 gatagaggat agcaataaag ataaagaaaa aatgacattt gaagaaaaca tagattctga
     2761 atttatagat gcttataata atgcttatga cgaaatacaa gaacaattaa aaaataatta
     2821 cactactcaa ggatttgaga gtgctatcaa aggagaaaga tttgacacat caactatagg
     2881 taatgttaaa tatgctaatt ggtttaaaga aggatatgat aatggtaagg ttaagttacc
     2941 taaagcaaaa gaaagtgtat ataatcaagg ttacaatgaa gaagatttta gtataccaga
     3001 tgaaatgaag tctattgaaa ctaagttaaa aggtgtatat gatgaaggtt tagaaaaaag
     3061 agaagaagag aaaagtagaa atgctactta tggggttgga gctggaacag cagctgtagt
     3121 tgctggagta gtatataaga agaaaaaacg taaaaagata tagatgctag atataccaat
     3181 gatgacaagt gtataacaaa tataatttta agtataaata tttaaaatat agaagggaag
     3241 gttatatcta tgggatttaa ctttaggaaa agcataaatc taggaggagg actcaaatta
     3301 aatttaggta agaagagtgt aggtataagt gctggtgtta aaggtgctag agtgtcagtt
     3361 aattcaaaag gaagaaaatc agctacattg agtatacctg gaactggaat atcatataca
     3421 aaaactagta caaagaaacg taaacctaaa taaatatttg atatactaag aaatagactg
     3481 ttacaaatag tctatttttt agtatataga tttaaaaagt gacaaaattt tgaaatataa
     3541 actaatttca taaaggaaaa tattctttta tgtagaatta taatatttaa gtaatattat
     3601 aaaatttata ggaggatttc attatgaaat taagcaaaac actattggca atagggatgt
     3661 ctgtttcatt attgattgct agttgtccaa catcaaacgc tcttagttct attaattcaa
     3721 taaaaggtac aaataagtat gaaacagcag gtttaatagc agataaacaa aattatgata
     3781 ttgcaatact tattaattct gaaaatggat tatctgatgg attgtcagca agtggattgt
     3841 caggagctaa aaatgcccca atattgttaa ctaagaaaga tacaatccct tcagaaacat
     3901 taaaaagatt agaaaaggta aaagaagtat atctaattgg aggaaaaaat tcgataggag
     3961 catcagttca gaaaatatta agtgataaaa aaataagcat aaatagaata agtggaactg
     4021 atagaataga tacaagtcat aaagttgcaa aagaaatagg aagagtaagt acggttaaaa
     4081 aagtaatatt aactaatgct tttaaaggcg aaccagatgc tatgagtgtt gcttctatag
     4141 cagttagaga taaatctcca atagtattaa ctgatggaaa tactacaagt tttgctacag
     4201 atggattaga aacttatgca attggtggaa aagtcagtat gagtgataag ttaataaatg
     4261 aaactaaagc aacaagaata ggtggagtag atagatacga tacaaataaa caaataataa
     4321 ataaatttta taatggctca aaagaatttt acatagcaag tgggactgat ttagtttatt
     4381 ctttagttgg ttctacaatt gcacaaaaaa cacctatagt gttagttgga aatggaagta
     4441 ataagagtat attaaaaaat gcaagtaaaa taactgctat tggaaatatt gatgatgttg
     4501 ttataaaaca atgtttaaat ataactaata atgttgggga ttcaaataca ggtattgtaa
     4561 aagatactaa ttcaaataat aagaacaaac caagtaatga gcctagctct aataatcctg
     4621 gattaactga taaaagtaca gtatatgtaa ctcctaatgg aaaatcatat catctaacaa
     4681 aaaattgttc tacactaaaa agaagtaaaa caataaataa agtaacatta aaacaagcta
     4741 agtcacaagg taaaatagac ccttgtaatg tttgtgtaaa ataatgtatt agccaaatat
     4801 atattaaaaa agacaattaa ttaagattgt cttttttatt gaagaaatat aaatttatat
     4861 tatttattat taaaaagaaa attattaatt ataaaaaata ataataaaga cgataagtat
     4921 taaaatttta tgtttccaat atttatgata taattaatat ggcaaggaat aaatatttga
     4981 aaaagctatg agtggtgttt ccatggaagt ttcttcactt tcttatgaga ggaggtgaaa
     5041 actatggaaa atctaatgat gagtattata actggtgtta tagccagtta catttacgat
     5101 aaattaaaga gccactctga gcgaccaact aagagtggct gggaacttaa tataaagttc
     5161 cagaaaagta aatcttcaaa ataatttgtt gaaggaaaca tcactcaaag ttaagtaaaa
     5221 tatgtttcct tgcttttatt atatcacaaa ctaaaaatat taacacaaaa ttttgtgaat
     5281 atatacttat tattgttcta aaaaaatatg taaataattg atttgattgc atggaattat
     5341 tgatgtaata tttaagttaa atatacacaa tttcaattaa agaagataag tactataaat
     5401 tcatatcaaa aaaatacata aacttttaag ttataatttt ataaaaagtc ttttagtatt
     5461 attatttcaa gaggaattaa catatgaaaa aataacttca ttagaaaagt ggataaaaaa
     5521 ctggtaagaa gatgactaaa gattacatat actattgctt aaattaaaaa tttaatataa
     5581 tttataatgt atacaattta ttataaaaaa tttaatttta attttctatt tttgatacta
     5641 taatatataa tcttgataat ttaattaaaa atctagattt tacataccaa actggtttct
     5701 tatttcgcta tctttactta tgaactcata tattcctata aaggttattt tagaaaaagt
     5761 tggttattta aacattaata ccacaatttc tatagattgt ttgttgaata atctataatt
     5821 atattatgga ctaaaaaatc caatgagcta atttattggt tttttacatt tgagagatta
     5881 caacttatta gtttgtatat ctcttttttt aataaaatca ataagatata aaactattgg
     5941 catatttaat gaca
//
LOCUS       DAELJO010000212         5926 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELJO010000212 DAELJO010000000
VERSION     DAELJO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.214"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..380)
                     /locus_tag="KPB88_003369"
     CDS             complement(<1..380)
                     /locus_tag="KPB88_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0055607.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFV"
     gene            complement(528..1319)
                     /locus_tag="KPB88_003370"
     CDS             complement(528..1319)
                     /locus_tag="KPB88_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0055608.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1355..2011)
                     /locus_tag="KPB88_003371"
     CDS             complement(1355..2011)
                     /locus_tag="KPB88_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055609.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2004..2969)
                     /locus_tag="KPB88_003372"
     CDS             complement(2004..2969)
                     /locus_tag="KPB88_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG0055610.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3075..3181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3471..4415)
                     /locus_tag="KPB88_003373"
     CDS             complement(3471..4415)
                     /locus_tag="KPB88_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0055611.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            4526..4993
                     /locus_tag="KPB88_003374"
     CDS             4526..4993
                     /locus_tag="KPB88_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055612.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5163..5762)
                     /locus_tag="KPB88_003375"
     CDS             complement(5163..5762)
                     /locus_tag="KPB88_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055613.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         1933 a          984 c          633 g         2376 t
ORIGIN      
        1 acaaaagcac tataaaaaat tagtattggg cttattctta gtttttttac tttagaaatg
       61 gttaagtata taaaagtaga catattaatt atgcttaagt taagcaatac aatttggaat
      121 ttttgaattt ttaaatttac tactcctaaa atacttacaa aaactaatac aagacaccct
      181 actttcaata tatttataaa ttcatctgtt ttaaagctac ttatataaac tatagaatat
      241 ccaaatatcc aaagtaaaat tattctatac atatttatag aaactgtata caaatttata
      301 tttaaaagtg caataaccaa aaataaccca ttagctagta tatagacctt attcttactt
      361 aaagttcctt tagattccat attccccacc acttcttcat ttttatatag ttttacctat
      421 aaatatgtta ccacaattct tccaaaaaat ttgtcacttt ctagttataa aaaaaaatga
      481 gtaaatattt ttaattttac tcattttgta gattttgtat attcagttta aaaagctgga
      541 actatacttc ctttatattc atcttgtata aattttttaa cttctttact attcatagcc
      601 tctgataaag ccttaatctt atcactatct ttattatttt ctctacaagc tattatattt
      661 acatatggag aatcacttga ttctattact attgcatcct tagtaggatt taagttagct
      721 gttaaagcat aattagaatt tattactgct ccgtcaacat cttttaatac tgttggtaat
      781 tgctctgcat tcatttcttt tattactata tttttaggat ttttagttat atctttttta
      841 gttataagct ctccatcttt tacttctatc aatttatttt ttgctaataa tttaagtgct
      901 ctagctccat tagttgcatc atttggcaca gctataacag ctccatcttt taattcatct
      961 aatgacttaa ctttttctga atagaatccc attggctcta tatgaacttt agatgtataa
     1021 gtaagtttat atcctttctc cttcacagtt tcctctaaaa atggaatatg ttgaaagaag
     1081 ttagcatcca aagacccttc atctaaagct gtatttggaa gtacataatc atcaaatatt
     1141 tttacttcta aatcatatcc tttttctttt aacaaaggtt ttgcaacttc caatatcttt
     1201 gcatgtggat ttgatgaagc tcctactagt atttttttat cctctttgtt tgaacaacct
     1261 actgctgata ttgctattgc tgacaccaat gctactgata ataatttctt taatttcatt
     1321 tttactcccc ctctttaaaa tgtttataat taatttattt tagtttctta taagccagat
     1381 ttcctactcc ctggattatc tgaactaata ttataagtgc tattacagta tataccatta
     1441 ctgctgtatc aaatctttga tatccataaa taagtgctat attacctaat cctccagctc
     1501 ctactgcacc tgccatagct gtataaccaa gtatagatat tattgataat gtaattccag
     1561 atactattga aggcatagct tcctttatca taactttaaa tactatttgt ctttttgtag
     1621 ctccaaaaga ttttgctgct tctatcaatc ctttatcaac ttcatttaat gagctttcta
     1681 taattctagc gataaacggt gctgccccaa tagttattgg cactattgca gcagtttccc
     1741 ctatacttgt tccaacgatt aatctagtta ttggaatcat tgcaactata agtatcataa
     1801 atgggaaact tctaaatata tttactataa atcctaatgc actatttatt gtgctatttg
     1861 gttttaatcc atctggtttt gtaactacta atatgattgc taatataaat cctaatattg
     1921 ttgccactat agttggtact ataaccatat acaaagtttg taacaatgca tttggaaata
     1981 gtgttgttaa aaagtcaatt aaactattca tttatcattt cctcccatct tatgccctta
     2041 ctagttagat attgttttac ttgctctcca cttttatctg aaatgtttat aataagtgaa
     2101 cctaaaatat catctttaaa ttgttctaat ttcccaaata ttatagacac atctatattt
     2161 agttctcttg ccatagttgt aataatagct tcattagaaa tatctttagg gaatagtatc
     2221 tttatatttg ttccttttgg caatattata ctttcttcac ctattaattt tctaagtcct
     2281 ttagtatttc ttaagaatat ttcttctgta tctcctacct ctaatacttc tccattttcc
     2341 attattgcta ctcttccaca aatctgcttt ataacttcca tttgatgagt tacaactatt
     2401 attgttattc ccaatttttt atttatatct tcaagcaatg aaagaattga ctttgtagta
     2461 tttgggtcta atgctgaagt tgcttcatca caaagaagta cttgtggatt tagtgctaaa
     2521 gctctagcaa ttgcaaccct ttgtttttgt ccgccactta gatttctggg tttatcattt
     2581 ttcttttcag atattccaac tacttctaat aactctaaaa ctcttttttt aatttctgcc
     2641 tttgagtatc caaaacattc taatggcaat gctacattat caaatacagt ttttctttca
     2701 agcaatgaaa agtgttgaaa aatcattcct aactcttttc ttaaatctct catttgcttt
     2761 tcattcaatg actttacttc tttatcagaa acaagtacac tgccttcttg gtattcttct
     2821 aatccgttta tacatctcag tagtgttgat ttccctgcac cactatgacc tattattcca
     2881 aaaatttctc cagattctat ttcaatactt acatctttaa gcacttgaat ctttccataa
     2941 tacttattaa cattttttat gctaatcata aaatttcctc ccatatattc tgctcagttt
     3001 ttatgtttat cctctcttat gtattacaac aaaataaaag cccgagtata aactcaggct
     3061 tagaaagcta tttatactca tctatcaact atattagttg caggatttag caccaatgta
     3121 tataaaatat actggttgcc gggtttcaaa gggccagtcc ctccaccact cttgataaga
     3181 cgttatttaa tttaattcta ttatcatttg ttgctatgtt atatatgata tattcattaa
     3241 tagtatttgt caataaattt atttaaattt tttatttttt attatcgtta attaactatt
     3301 tttaattagt tttctagctc ctaacacttt atattttaat gtctatataa ctatagaaca
     3361 tcattataag aaataaatta aataacatca atctatctaa atcatctgta aagtaaatta
     3421 agaaaattaa cattttatcc aaactctatc ctactattaa ataaaataat ttaaatacaa
     3481 tgatgattat atagctctag tactttagac tttatcaaat gtttaagttc tataggttct
     3541 attattgtag catttttccc aaaagttaaa aaatattcag ccacccagtt aagtgtagat
     3601 tttggtatat acatatctat gtatccacct ttagacaaga ttttcaaccc tcttgccaac
     3661 aaaaattctg tttcacaacg ttttactccc tcatctgtca aattaatttt aatatgataa
     3721 tctgtaccta cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc
     3781 ttacttgata agttttctat actcaaaatc ttcacaattc tgtctacacg aaattcctta
     3841 atagattctg attttataca gtaagctgga caataccaaa gtccattcat ggcatacaaa
     3901 ccaataggaa caatggtacg tacactattt ttttgctttg atgaatattc aattttaatt
     3961 gtaaattcat tcattactat attaaacaat tctttcaata atggtgttga acagtgccta
     4021 tctggtgtcc aaaaaacaaa tttacgcctt atattctcaa tattatattg aatatcattt
     4081 ggtatacaat ttaaaaattt ttttaaaaca gaaatagttt cttgctcaaa tggcaaatca
     4141 ttataatatt ctagtgactg atatgcaaag aatatagatt ttacctcact ttctgaaaat
     4201 gcaatcggtg gaagtatacg ctctttaagt acatgatatc caccagccgc tccaactttt
     4261 gaatataatg gaaatccaat actctccagc acctgtaaat ctcttagtat agtacgtttt
     4321 gatacagaaa attcttctgc taattctcct gctgtaaaac ttctttttgc atttattgtt
     4381 atcatcagtt caattaaacg ctcagctctt gacatattta tactcctaat ttaaatatag
     4441 tgacaataat tgtcactatt atatgataag atattcttgc atctaaagca ataataatag
     4501 taaagaaaga agggattgat actttatgga aaatgatact attaaggctg atgatattct
     4561 caattattgt ctatcaaact tagatgatgt tgtactaatg gatagttggg gggaacgagc
     4621 aatttactac aatcctaatg gtgttttaaa gcgaggggta tatgttctta ccattaagga
     4681 aaaagacagt aataatgata aaggttcgtt agttagtcgt ccaaatgtat accgtgtgaa
     4741 tataggatta aaaaaagaaa cttttattga aatgtttgga tatattccaa agcgtccagg
     4801 tgtaggtcaa atagttgata tggattttga ttttacaaag ttggacacaa tcatgccaca
     4861 tcctatctac tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga
     4921 gaactttaaa actttaatag gagaatctta caattttgca aaacaaaaat ttaaaaagag
     4981 aaaaaacaag taaaaacgta tgaaaataat ccctaaataa attataataa aaatattaag
     5041 gttacaagat aaaaattaat atcttaataa catgcaaaaa agcgaagcca aaaaatgtat
     5101 tatttataag gcttcgctat atctcaaata atttatttca aatttcaaaa ctatttattc
     5161 tattatttct tattatttcc tgttattcct aatctatcat tcatatcttt tatcattttt
     5221 ctaaaaccac tacctatatc atttccaaca tcttcagttc catgaactaa ttttataaag
     5281 tcattttgac cttttttcat atcttcatca gataccttat ctaatttttc tattctctta
     5341 tctatgtctt ttactaatct ttccccttca gcgattaggt catcattcat tttctttaac
     5401 tctgtatctt ctgttttacc atctttttta agagatgtta caaatgattg tattttatct
     5461 ctactatctt tatatgcagc ctttacatca cttagatatt tttcatttcc tggatattct
     5521 ttatctttaa actgattatt tatactttct ggagtatcat aaatagaata tctatttaga
     5581 cctgctatat aatcattata gcttgttgaa taataattca tatatccttc attattattt
     5641 acagattcat taggattttt tcctgtattt tcacttggtt tttcattatt tttagtacat
     5701 gcagttatac ctatagctaa tactagtgct aatcctacaa ataaagttct ttttaatttc
     5761 atttttatta ccctcctaga tgttttatac tataaaaaat tttaataatt ttctttctta
     5821 atttagtatt tctatatttt tatattttat ttactaaaat taaacaagtt ataatttatt
     5881 tttttggaaa tcataatgaa taaataatta aataaaaaaa ttataa
//
LOCUS       DAELJO010000213         5894 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELJO010000213 DAELJO010000000
VERSION     DAELJO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.215"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(312..2144)
                     /locus_tag="KPB88_003376"
     CDS             complement(312..2144)
                     /locus_tag="KPB88_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055614.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2137..4383)
                     /locus_tag="KPB88_003377"
     CDS             complement(2137..4383)
                     /locus_tag="KPB88_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055615.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKEKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4451..4912)
                     /locus_tag="KPB88_003378"
     CDS             complement(4451..4912)
                     /locus_tag="KPB88_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0055616.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5217..5783)
                     /locus_tag="KPB88_003379"
     CDS             complement(5217..5783)
                     /locus_tag="KPB88_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG0055617.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1889 a         1087 c          634 g         2284 t
ORIGIN      
        1 atataatcat caactattaa aattaaattc tatttataat aaaatttaaa gaataaaatt
       61 aaatatacat tttgataatc acctccaaca ttttccttac tacatattat aataaaataa
      121 acactttgta aagacataaa attatgtggc tatctcaaaa tatagataat cctctttttc
      181 attgttaaaa tgataaaaat acactttatt tcatacacaa aagagtgcct tatgaattaa
      241 aaagttcatt ttaaggcacc ctcatttatt ttatcttatt aattacatgt ctatgctaag
      301 atagtttttt gttacattgc ttcatcttct tcaaattgag aattgtaaag attggcatag
      361 aatccgcctt tctttagaag ttcttcatgg tttccttgtt caactatatc tccttcattc
      421 attacaagaa tcatatcagc atcacgtata gttgataatc tgtgggctat aacaaaactt
      481 gttctacctt ccataagatt atccatagct ttttgaatca atacttctgt tctagtatcg
      541 acagaacttg tagcttcatc taatattaaa attttaggat cagctaaaat agcacgagct
      601 atagtcaata attgtttttg accttgagaa acattacttg cttcttcatt taatttcatc
      661 ttgtatccat ctggtaatgt cttaataaat ctatgaacat gtgcagattt agcagcttct
      721 ataacttctt catctgtagc attcaactta ccatatctta tattttccat aattgaacca
      781 ctaaataacc atgtatcttg aagaaccata ccaaacattt ctctaagctc actacgatta
      841 aaatctttaa tattatgacc atcaatcagt attgagccgc tattaacatc ataaaatctc
      901 attaaaagtt taactattgt agttttacct gctcctgtag gtccaacaat agcaactttt
      961 tgaccaggtt taacatttac tgagaaatca tttattatag tcttgtttgg gttatatcca
     1021 aagttgacat tttcaaagtc aattttgcct tctaaaccat ctattgaaac tgcattttca
     1081 acaatttggt cttcttcttc ctcttctaaa aactcaaata ctctttcaga agctgctgct
     1141 gttgattgca ataaattggc aacctgtgca atctgagtca ttggctgtgt aaagtttctt
     1201 acatactgta taaatgactg tatgtcacca acctcaattg ttttcttaat tgcaagccaa
     1261 cctcctaaga ttgataccat tacatatccc aagtttccaa taaacatcat aagtggttgc
     1321 attatacctg ataaaaattg agatttccac gctgaattat acaggctatc atttaattta
     1381 tcaaactcct caattacttc atcttcacga ttaaatgctt ttactattgt ttgaccactg
     1441 taagtttcct caacttgacc atttacattt cctaaatatt cttgttgata tctaaagtat
     1501 ttttgtgatt tctttattat tcttgaaatt acaaacattg atattggtaa tattaaaact
     1561 gccactaatg tcataattcc acttatagat aacatcatta tcaaaacacc tatcatagtt
     1621 gttactgatg ttataagctg tgtcatactt tgatttaaac tttgatttaa agtatctata
     1681 tcattcgtaa ttctcgataa tatttcacca tgtgtcttag tatcaaaata cttcattggc
     1741 aatctatcca attttgctga aatctctttt cttaaattat aggatacttt ttgagaaatt
     1801 ccactcatta taaatccttg tataaaagag aacagtgcac ttatcaaata aaggaaaagt
     1861 aaaaaggtta agatttttcc aattgcatta aaatctatac ctacgtttcc tccagaaact
     1921 ttactcacta gcccttcaaa tatctttgta gttgcctttc ctaaaatttt tggacctact
     1981 atagaaaacg acgcactacc aatagcaaat acaattacta gaattattga tattttaaat
     2041 ttagataaat atgcaattag ttttcgcata gtccctttaa agtcatttgc tttttgtcct
     2101 gctcccatag attttcccat aggacctctt cccattctac tcattagcta gctcctcctt
     2161 tgaaagttgt gataaagcta tttgctcata aacttcgcaa ttttttagaa gctctttatg
     2221 tgttcctatt ccaactattt ctccctcatt taaaacaatt atctgttctg catttaagat
     2281 agtactaatt ctttgtgcaa ctattagaac tgtactttca gcagtttttg attttaaagc
     2341 cttacgaaga gctgcatcag tttttaagtc aagtgctgaa aaactatcat caaatatata
     2401 aacttctgga ttttttgcta ttgctcttgc aatagaaagt ctttgttttt gacctccaga
     2461 tacatttgtt ccaccttgag aaatttcagt tttaaaagct tccggtttag aatctataaa
     2521 ttctgtagct tgagctattt cagctgctgt tctcatttct tcatctgttg cgtcttttct
     2581 accatatttt aagttggatt ctatagtacc agaaaacaat actccctttt gaggtacata
     2641 tccaattttt tcccttaaat catgttgaga aacatttttt atattaactc caccaagtaa
     2701 tatttctcct tcagtaacat caaagaaacg aggaattaaa tttataagag ttgacttacc
     2761 actaccagta cttcctataa aggcagttgt ttctcctggc tttgctgtaa aagatatatt
     2821 tgttaatata ggttcttctg catctggata catgaacgaa acatttttaa attcaacaag
     2881 acccttttta tcttccttaa actcttgtgc ctctgatttt tctgggtctt ttatgcttat
     2941 atctgacact aatacttcat ctatacgtgt agctgaaacg gatgctcttg gaagcataat
     3001 agataccatt gatatcatta agaatgacat tattatttgc attgtatatt gtataaatgc
     3061 catcatatct ccaacttgca ttgctccaga atcaacactg tatgcacctc tccaaactat
     3121 aagaactgtg ataaggttca ttataagcat catagcaggc atcatacatg tcataactct
     3181 atttacaaaa agatttgttt tagttaaatt tttatttgct ttatcaaatc tttcttcttc
     3241 atgtttttca gtactaaaag ctctgataac tggaatacca gtaattattt cacggcttac
     3301 taagtttaac ttatcaacaa gcttttgaac cagtttaaac ttaggcataa ccactgagaa
     3361 taacactaca attaatgata agatagcaac tactgctact gctataatcc aggccattga
     3421 agtatttgtg tttaaaacct ttatcactcc acctatagcc attataggtg catagaatac
     3481 tattctaagc aacataacca tcaacatttg tatctgttga atatcatttg tactacgagt
     3541 aattaatgaa gctgtagaaa attcattcat ttcagtattt gaaaatgaca taacttttct
     3601 aaatactcca cttcttaaat ttctacctac acttgctgct actcttgctg ctagaaatcc
     3661 tactgttatt gtagctacca tactaatcat tgctatacca agcattttag caccagtaaa
     3721 taatatataa ttattttgag ttttttctgt atcaatacct attttctcat attctgacct
     3781 tacatatgaa accgagcttt gtgttatcat actctctggc atactttcaa agcctttgct
     3841 catctcttta gtcaatgtat ctaactgttc ctttggcatt gctgataaca atttaaatat
     3901 atcaacatct ccactttgag tagcttgtgg tggaagttta ctcattaact gagctttcat
     3961 agcttttacc tctttactat cgccctcaag ccctgatact attaaaatag ctttaccaaa
     4021 aataacattt aaatcatcta tagtgtcttt gtctttagta tttaactcat aaagagattc
     4081 atcttttaga cctgggtact tatctttata cttaacataa tcctttttat tcagcaaagt
     4141 ataattatct agtactttct ccttactact tttatccata aataaagtaa ttttatttaa
     4201 ttcactctct ctaatcacag atggaacaga gttttctact ccaccttgtt gtatacctac
     4261 atttactata ttggatgtgt attctggtaa agaaaggtca cataccgctt gaattaccaa
     4321 tagacagact attattacta ttgataatgc cgatttcttt aaatatttaa ttaatttaaa
     4381 catacatttc ttcccttctg taatattaat tagtctaata acattaaatt gaaaaacatt
     4441 attttaaact ttaaaaatat ttttcatctt catgcttatg acatcctgct tttaaattat
     4501 ctcgtacttg cattagaagt cttctaagta ctattttttc ttcttcacta aatcctaaaa
     4561 aaaactctct ttccaattcc ttgttaaatt cttcaacttt attacatatt tctaaacctt
     4621 tgtctgtcaa aaaaatccta gatactcttt ggtccttttc atcttgtttt ctttccacaa
     4681 gtccagcttt ttccatcctc tttatcataa ctgtagttgt agatgctttt actttccttt
     4741 tatctgctaa ttctttttga ctttgcccat ctttcatctt taaagaaaaa agtatttggg
     4801 gttgaccatg atataagcct aatttatcaa aataaaaatg agttcttata aaatatattc
     4861 gcattaattg caagaaaaca tcatttaatt tttcttcaga attgctattc atacttgccc
     4921 tccttatttt ttatattact tagtcagcta agcattattt tactatactg catgcatttt
     4981 ttcaatatat ttataaaaaa ttcatataaa tttcatttat taaatatttt tatatcatat
     5041 agttataatt ttaataaata atagtaagat ttttaattag tcagctactt atatttttta
     5101 ttgcaaagct taaaataaac cttttcatat attttcaaat aaaaaagttg tcccaaaata
     5161 aattttgaaa caacatcata ttcaacagta tcttgattat ataacattat gatactctac
     5221 ttatcttcat atccatttac aacaacgtct aacttcccag tttcaggtga tataattaga
     5281 ccatgtacaa taataccatc tggaattaaa ggatgccctt ttattaaatc aatactatct
     5341 tttacagatt cttctgcaga atcgaatcca tgtaaccatt gttttgtatc aattccagca
     5401 ctacgaagtg taagtattac atcattagat attcctctat ctaaaatttt accaagtaac
     5461 ttatccgtat caacattaca cataccacat ccatgatgcc ctacaaccat aacctcatct
     5521 acatcaaatt cgtaaattgc aactaaaaca cttctcataa tacttccaaa aggatgcatt
     5581 atagtagctc ctgcattttt tattagtttc acatctccat ttttcaaatt catagcctta
     5641 ggcaaaagct cagttaatct tgtatccata caagataaga ctactatttt tttatttgga
     5701 tgttttgatg tcacatattg ttcatattct ttgttctcta caaatgattt attatattct
     5761 aatatctctt caagttttct catatgtaat atcaccctct ttaaaaattt ttttattatt
     5821 atatcactat taaagtacaa tatatacaca tataatttaa tttatattta ttaaattatt
     5881 ttataagatt atta
//
LOCUS       DAELJO010000214         5823 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELJO010000214 DAELJO010000000
VERSION     DAELJO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.216"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..390)
                     /locus_tag="KPB88_003380"
     CDS             complement(<1..390)
                     /locus_tag="KPB88_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055618.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTK"
     gene            complement(864..1748)
                     /locus_tag="KPB88_003381"
     CDS             complement(864..1748)
                     /locus_tag="KPB88_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0055619.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2039..2515)
                     /locus_tag="KPB88_003382"
     CDS             complement(2039..2515)
                     /locus_tag="KPB88_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG0055620.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVDLAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2782..3957
                     /locus_tag="KPB88_003383"
     CDS             2782..3957
                     /locus_tag="KPB88_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG0055621.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4038..4841)
                     /gene="proC"
                     /locus_tag="KPB88_003384"
     CDS             complement(4038..4841)
                     /gene="proC"
                     /locus_tag="KPB88_003384"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0055622.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4881..5465)
                     /locus_tag="KPB88_003385"
     CDS             complement(4881..5465)
                     /locus_tag="KPB88_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055623.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDTVKEA"
BASE COUNT         1948 a          988 c          650 g         2237 t
ORIGIN      
        1 tttagtaaat tttacggtaa gtaaaaacct ctctccttca aatcctatag caaatatact
       61 tacaagttgc tttgagaaaa taactcctaa tatagctaca actatgcata taccaactat
      121 tatatttaaa acattgttta aaaactttag tgcttgcttt tcaccttgtt tactacttat
      181 atcttgatac attggaataa aagtagtaac tatagcagta cctattgctg caaatataat
      241 atttggtata ttcatagctg ttaaataact ctctgtatat aagcctgtac cataaattga
      301 tgataatact aattctctac ccattccaag aatcttagag attatagtaa ctatcatcaa
      361 ataaaaagtt gctttagcta ccttgctcat tttatcactc cacttctaac atacctcttt
      421 ttaatcaagt aaaatttagc tcattagata taattctatc aactaatttg ccttgcttag
      481 ccctatcaaa atataattaa cacctatttc ttaatatatc acaaactaca ttattcttca
      541 tattttttta tttctccaat aagttttaca tttttcctat ataaattgta tagcttcaat
      601 tatattcaaa gaaatggagt tgctacccga taactttaaa agtaaaaaac agattttatt
      661 atctttttaa actatattct aacatatttt gtccatattg agaaatttta ctaaactatt
      721 atgtacttta ttaaataaat aaaataacag cacacctata aaatacaagt gtgctgttca
      781 taaacagatt ttataaaaaa ataattccta agtagagatt aacatgcttt ctcatactct
      841 catatataat gtgtataaaa atattaataa aaatgcttta attgttttaa cttacttgga
      901 tgtcttaatt ttcttattgc tttagcttct atctgtctta ttcgttctct ggtaactcca
      961 aatacctttc caatctgttc tagtgtttta gggtcatcat catcaattcc aaatctcatt
     1021 cttaaaactt gctgttcttg ctctccaaga cctgtaagta gttcttctat cttttctttt
     1081 aaattatgct gttcaacttc gtgtattaca acatctttaa aatcagcatc tgatggtata
     1141 aattcaacta aactactatc ctcatcttct tttaatggag tatccaaaga tacaacattt
     1201 ttatctcttc ttaataattc caacactttg tcaacttcta agccacatgc atctgcaatc
     1261 tcatctatag ttggctctct taataataca tttaaaagtt cttgtttctt tttctttaca
     1321 aaatttattc tctgatgaag atgtataggt atcctaatag tattttcgca atcgtcgata
     1381 tatcttgtta tactttgttt tatccaccaa gttgcataag tgctaaattt gtatcctttt
     1441 ttataatcgt acttttctac agcttttata agtcctatat ttcctgcttg aattaaatcc
     1501 aacatatcaa tactatctct tttatatctc tttgctatgc tcactaccaa cctataattt
     1561 gaatttataa atttttcttt tgctactgaa gatgagttga ttatttcctt agccagctct
     1621 acctcttctc ctgctgacaa cattttgtat tcctctattt cctttaaata cattttcatc
     1681 gcattcgata catttgatga acccacacaa atataatttt cataattatt ttctttttta
     1741 gtatccataa tcaaacccct tctattcata gaatttgcta aaacaaaaat aaataattta
     1801 aacctacata atctacaatt acataatatc ccattcttta aatgaattgt tttgtatatt
     1861 atgtatacaa atttataaaa actataccta taatattatt ctacttctaa cagcaatatc
     1921 ctccttatat atataattta ttttattata tgttgttata ttcttagttt tttgaatgat
     1981 atatgtctaa aaaaagactg tttactaaac agtctttttt tatatatatg ctcaatattt
     2041 aattttcatg atgatgatta catccacatc cacaattttc atcatggtga tgatgttctt
     2101 ctaaatcttt tacctgttgt tccatttcat gaaactcttt taccattata tcataagttg
     2161 gaagagctgc ctcagtatcg tattcttccc ctctccaatc agcgtacatc atagcatctc
     2221 cattagttaa gaccaatcta cctgacatag ttatattatc cacttcttct aaaaattcta
     2281 tttgtttttg taaaacttct ccttctaact ccacatcttc atctaaacat tgcactatca
     2341 ttacaggtgc ataataatct tcttcatctt taacatttac aacaatatct aaaattcctt
     2401 ctgcaccttc ttcaaattct gccaaatcaa catttgtatc aatcatatct tgaggtacta
     2461 ataaatcttc tattaaatat tttattactc tatcttttac taattctttt gacatttatt
     2521 tttctccttc cgattctata acattatcat aatcatttct atattatagt atactacatt
     2581 aattgttttt tcaatctctt acaaaaatta ttttttcatt atcttcctta tattcccttt
     2641 tttattacaa agttgtaatc gcattgattt aaaaaatagt ttatatttag tataatagaa
     2701 atgtagcaca tatttttatt caatatatta ataaaaaaat agattgaaaa tgtaatgtat
     2761 gttaaatcta ggaggaatat tatgaagttt aaaaaacaaa ttagttctat tgttgtgtct
     2821 actatgcttg tacttgggag tatgaacctt tcttatgcag acggtacaaa tgtagttaca
     2881 cttggagcta acttatcaga gtctcaaaag cagcaaatgc ttaattactt tggagtaaaa
     2941 aaagaccaag tcttagtttt agatgtaaca aatgcagaag aaagacagta tttacaaggt
     3001 gttgcaacag agggtcaact tggaaaggtt actatctctt gctcatacgt tgaacctact
     3061 aaaaaaggaa atggaattaa tgttaaaaca gcaaacttaa cttgggtaac tagttcaatg
     3121 attgcttcaa cccttacaac agctggtctt gaagatgcaa atgttattgc tgctgctcca
     3181 tatcctgtaa gtggtactgg agcattgaca ggaatcatga aagcatttga ggatgcttca
     3241 ggaaagaaac ttgatgaaac taagaaagaa attgcttctg aagaattggt agttacaggt
     3301 gacttaggag atgatatagg gcaagaaaaa gcaacaggtg taatgaatga tataaaaaca
     3361 gaaattgtaa agaatggtac aaaagatacc aatcaaatag caaatacaat aaataatgtt
     3421 gtaaataatt acaatattac tattacacca gaacaaaaag aacaaattaa aggtgtaatg
     3481 aaaaaaatag ctgaccaaaa ttacgattac aatagcatga aaaatacact agataatgta
     3541 tcagataatg taaatgaaca attaaagaaa ttaggtgaaa gtgttaaggg ttctggtata
     3601 ctagatacta ttggtggatg gtttagtgga ataggagact ggttctctgg tttattctca
     3661 ggcggagata aaaaagattt gggaatctta aataacacta atgatgagtc tcttggctct
     3721 gatgctgtaa taaattctac agaaggtgtt caaataaatc cacaaaataa tactaattct
     3781 aacaatgata gtgatacaaa tagctcaaaa aatgatactt caagtaataa tgaaaataac
     3841 gatagttctc aaacaaatga aaacaacaca acaaataatt cagaaaatca atcaaataat
     3901 aatgactcag ttggtttctt tgaaaaaata aaaaattggc tttcaggctt attttagaaa
     3961 ttaaacaaat aaaaagaatt gcctaaaatg gaaattttct ataaaaggca attctcttta
     4021 tttacaataa atttttatta ctcactcatt tttttagatt tttctataca tgcacatatt
     4081 gcttttatca cagaagctct aaaaccttct tcttcaagta ctttaactgc ttctatagta
     4141 gttccaccag gtgaacatac catatctttt aattcaccag gatgttttcc tgtctcaagt
     4201 accatttttg cagaacccat aacagcctgt gatgcaaact tatatgcttg tggtcttggc
     4261 attcctgcaa ttactgctgc atctgctata gcttctataa ataaaaatac atatgctggt
     4321 gcagaaccac ttgcaccaat tacagcttct ataagatact ctggtactac ctcagtattt
     4381 ccaaaagaat taaatacttc tgtaactgct tctaagtctt ctttgtttat atttttattt
     4441 atagaaagag aagacatggc ttcatttaca agagctggag tatttggcat agttctaact
     4501 atttttttat cttctcctat aatagactct atatctttta tagattttcc tgctgctatt
     4561 gtaactataa ttttattgtc atctataaag tcttttatct cttccaaaat atcatcatat
     4621 atatctggtt taacagctac aattactata tcagaggttc tagttacttc cttcgagtct
     4681 tttgtagtat ttattccaaa atttgcatct attctatcga gagttccttg actatatgct
     4741 gatgctataa ccatattagg ttcaactaat tttgaattta ctattccccc tatcatagcg
     4801 cttgccatat ttcctgcacc tatgaatcca atttttttca ctttagtacc tccttattga
     4861 ctaatagtaa tcgttaaaat ttaagcttct ttaactgtat cagacttttc aaatggattt
     4921 ggtatactta ctaccaaaat tttcactacc ttattagtat agttgaccca attatgattg
     4981 ttcttttcac tattatagtg tattgtgtct cctggataca tttctatttg ttcatctccc
     5041 aaaaacactg taagagtacc ctctaataca tacacaaatt cctcaccttc atgtacgtaa
     5101 caacaaattt cctcactact cttacttgga agaatttcaa ttaatcttgg tagcattgtt
     5161 ttttccttta aactagatga taaatgataa tgtataaatg ttgagttttc tacatcaaat
     5221 acttcttttt cataacttct taatacagct cttttatgct cttttggctt cataaaaaag
     5281 taagttaatt ctacatctag tacacttgct atttttccta aggaatctgt tgccacactt
     5341 gttaatcctc tttcaagttg tgataaaaaa ccaattgaca agtttgtttt ttcactcata
     5401 tccttcagcg tcatttgctt ttgagttctg agttgcttta tttttgctcc tatgtcctta
     5461 ttcaaaacca tcacccctaa ataattttaa ttttttcata atataaatac aaaaatataa
     5521 atataggtac tgaacctatc tttatttcat tttaaaaaat ttttataagt attactataa
     5581 tttatcaatc tttttttgtc tacattttta cgattattta cgatttattt ttcacatatg
     5641 tatatttaat cttgccaact aaagatacat tttttataag catttactct ttatataata
     5701 aaacttaagt tgttcatgta gtcttcttct ggtttaatta ttaaaatttc aaattatatt
     5761 attatttcta cacattatta catcctttag tacaattata acataatttt acaatttaat
     5821 gat
//
LOCUS       DAELJO010000215         5812 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.217, whole genome shotgun sequence.
ACCESSION   DAELJO010000215 DAELJO010000000
VERSION     DAELJO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.217"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            101..667
                     /locus_tag="KPB88_003386"
     CDS             101..667
                     /locus_tag="KPB88_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0055624.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGDGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            707..1288
                     /locus_tag="KPB88_003387"
     CDS             707..1288
                     /locus_tag="KPB88_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0055625.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1345..2262)
                     /locus_tag="KPB88_003388"
     CDS             complement(1345..2262)
                     /locus_tag="KPB88_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0055626.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2392..4023
                     /locus_tag="KPB88_003389"
     CDS             2392..4023
                     /locus_tag="KPB88_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0055627.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKTPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4026..4973
                     /locus_tag="KPB88_003390"
     CDS             4026..4973
                     /locus_tag="KPB88_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG0055628.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5258..5521)
                     /locus_tag="KPB88_003391"
     CDS             complement(5258..5521)
                     /locus_tag="KPB88_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG0055629.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMKVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5620..>5812)
                     /locus_tag="KPB88_003392"
     CDS             complement(5620..>5812)
                     /locus_tag="KPB88_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055630.1"
                     /translation="DFKNIPEYSDEEFYVTVYKDESSDSGYYALLENKEERVVWDGEV
                     VANNIFNNLWIVVNKVKTG"
BASE COUNT         2088 a          792 c          979 g         1953 t
ORIGIN      
        1 atatagccaa cttagcctaa atacgtatat aatatagcta atatggttct tatacttgat
       61 accatcaata aaactaaaaa tataaaaagg agaacttaaa atgagtgtta gtttacaaaa
      121 aggacaaaga gttagcttag taaaagataa taatcctgta aaaaatctag tagtaggctt
      181 aggatgggat atgaataaat tgggtaaaaa aaattatgac ctagatgcat ttgcaatagc
      241 attgactaac caagataaaa tgaatgttag aggtgacttg gtttattttg gaaatcttaa
      301 acacccttct aaagctatta tacacactgg agataactta actggaaaag gtgatggtga
      361 tgatgaacaa ataattgtta atttagaaga catacctgag tatgtacata aaatagtttt
      421 tggagtttct atatacaaag ctaaaaaaag agaccaggat tttggtcaaa tgaacaactc
      481 ttttataaga ttaattgatt caaattccaa gcaagaacta tttaaatata atcttcaaga
      541 agacttttct ggtaaagtaa cagttttggc aggagaaatt tatcgtagaa atgaagaatg
      601 gaaatttaat gctttaggaa ttggtcaaaa tgaagagctt cgtgaattaa ttaatacata
      661 taagtaataa ttacaataaa aaatttataa tgaggagttg gtttaaatgg gtataacttt
      721 agcaaaagga caaaaagtaa gtttaacaaa aagtaaccct ggtctaaaaa aagtaatagt
      781 aggactagga tgggatatta ataaatatga tggtggcttt gatttcgatt tagatgcctc
      841 tgcattttta actggaactg atggaaaagt aacgaatgat ggagacttta tattctataa
      901 taatttaaaa cacgcttctg gagctgttga atatatgggt gataatagaa caggtgttgg
      961 tgatggtgat gatgaacaaa taaatgtaga cttatctaaa atacctcaaa atattgctaa
     1021 aatatcattt agtgttacta taaatgaagc aattacaaga agacaaaatt tcggacaagt
     1081 tgaaaattca tatataagaa tatataatga agaaactaat gaggaactta ttaaatatga
     1141 attgggtgaa gattttagca tagaaacagc aatagttgtg gcagaactat atagacataa
     1201 tggagagtgg aaatttaatg ctttaggttc tggatttgag gatggacttg caggattgtg
     1261 taaaaatttt ggcgttaata taggctaata tatctaaata aaaataagag tatattgata
     1321 tactcttatt tttatttaga tatattataa actgtttaca cttctactct tctttctcgc
     1381 ttgttttttc ccatattcta ctgaaatatc aaaaaatgct gaaatatttt ctgtaaaaaa
     1441 cttgcttcta tgatatgcaa tatcaaatgt cctatgtaaa ttaatacctt caatatcaca
     1501 agcccacaaa agatgctttt ttaaataatc ctcaataagt aattcggata taactgcaat
     1561 gccaaaatta tctacaactg ctcttaatat agcttcagag ttataacatg accatctaat
     1621 attcattggg atttttaatt cttttaattg cttttctaat tgtgctcttg taccacttcc
     1681 taattcacgc ataattaatg gttgatttga aagttctgaa acttttatag agtctctttt
     1741 gtagaactca tgcttgtgtg aacatataag tacaagatta tcattaataa tgctttttgt
     1801 aacaatatct gggtgcttaa tttttccttc tacaatcgca atatctaatt cactattaag
     1861 taattttttt gcaatcagtc ttgtatcttc tacaaatact tcaggttcta taagaggatt
     1921 taccttgtat aattctaaca ttattggact tattacacat gtaccaacag ttactgttgc
     1981 cccaactctt atacggatat tttttgcaca atgcttcata caatcattca tttcttcatt
     2041 tacagctaaa agatgcctcc catatccaag aagttttttc cctgcctcag ttaaatataa
     2101 tttttttgaa attctatcaa atagcttaac accatactct ttttctattt ctgaaatagc
     2161 ttggctaact gatgactggg taatatacat atttcttgct acttcactca ttttaccaca
     2221 ttcagcaacc ttaacaaaga tttcaagact acgaattgtc atattatcac aaccttagca
     2281 aacattagta aatgcttatg attttattaa tattttagta ttttacaaat atattgtcaa
     2341 gaagtataat tttattatag agttataaag tagattggag tgtgatatat tgtgaaagta
     2401 ttaattatag gtggagttgc tgctggaaca aaaactgctg caaaaataaa acgtgaaatg
     2461 ggtgataatt gctctgttac aatactaaat aaaggtgatg atatttctta tgcaggttgt
     2521 ggtcttccat attacgttgg taagataatt gaagataaat catctttaat cgtaaataca
     2581 cctgaaagtt tttctaaact aacaggagca gaagttcgtt gtggagttga agttacatct
     2641 attgatagaa atgcaaaaca agtaactttg aaaacgcttt caacaggaga aaaagaaaat
     2701 ctaagctatg acaaacttgt aattgctaca ggggcagacc caataaaacc accaatacct
     2761 ggaatagatt tacctggagt attctttatg cgtactccaa atgatgctat aaaactaaga
     2821 gattctattg aaaacggtat taagagagct gttgttattg gtggaggttt cattggtctt
     2881 gaaattgctg aaaatctttc tgcaatgggt atacgtgtaa gtgttgttga tatggctgaa
     2941 catgttatgc ctggatttga tactgatttt gctgaatacg ttgaaaatca tatggctgat
     3001 aaaggcataa tgatttttac aggtgaccag gttgtaggta ttgaaggtga aaataaagtt
     3061 gaaaaacttc gtactaaaaa tagagctatt aaaacagatt tagtggttat gtcagttgga
     3121 atccgtccta acacaggttt tctaaaagac acaggtttag aatttgcccc taataataca
     3181 attattgcag atgaatatat gcttacaaat gacaaagata tatacgtagt tggagattgt
     3241 gcttttgtaa aaaattcttt aacaaataaa cctacatggt cacctatggg gtcatctgca
     3301 aatcatgagg gtagaatttg cgcacaaaat atatctggaa aatctaaaac ttacaatggt
     3361 gttcttggaa caacagtagt aaaattacct gagctcaatg caggaaaaac aggtctaagt
     3421 aaagaaatag ctgaaaaaga aggttataat gtttgctctg taaccatagc tactgatgat
     3481 aaagctcatt attatccagg tgcatctaat tttattataa gaatggttgc tgaaaaaaat
     3541 actagaaaat tattaggtgt tcaagttatg ggacctggag cggttgataa aatagtagat
     3601 atcgctgcta ctgcaataac tttaggcgct gacctttatt cattagaaag tatggacctt
     3661 gcatatgcac cacctttttc aacagctatt cacccttttg tagtggcagt aaatgtttta
     3721 caaaataaac ttaatggaga acttgatagt gttttacttg acgaaattga ggattttgac
     3781 agttggacta aacttgatgt ttcaaaaact ccttctattc ctagtttacg ttatattcct
     3841 gttggtgaaa ttaatggaga aattgaaggc cttgctaagg atgaaaaaat tcttcttata
     3901 tgtgctaaag gtaaaaatag ctatatggcg caaaatcgct taagacgctt tggctataca
     3961 aatacgaaag ttcttgaagg tggaatacta ttttatccaa atcttaaggt aatggaggag
     4021 taaatatgag taatgttaca gcagaacaaa aaaaggaagt aaaaggtaga ggttttctac
     4081 caagcaggga tggagaacat tttgcagcac gtataataac tgtaaatggt gtaataaatg
     4141 catcacaagc aaaaaaaatt gcagaagctg ctgaaaaatt cggaaacggt cagttagcat
     4201 ttactactag actaacagta gaattacctg gtgtaaaatt tgaagatatt gaagctctaa
     4261 gtgaatttat tgctagcgaa aatctaataa caggtggaac tggatcaagg gttcgtcctg
     4321 ttgtggcttg taaaggtact gtttgtgttc atggtctagc tgatacacag gcacttgctg
     4381 ctgaaataca cgaagaattt tacaaaaatt ggtatgatgt aaaactacct cataagttca
     4441 aaattggtgt tggtggatgc cctaataact gtattaaacc tgacctaaac gatcttggaa
     4501 ttgttggtca acgtgtacct gactatgatt cagagctttg tgttggttgt aaaaaatgtg
     4561 ctgttgtaga ggtctgtcct gttaaagcag ctaaattaac agataaaggt aaacttgaaa
     4621 tagatagtaa tctatgtaat aactgtggta agtgtataga aagttgtaat tttgatagta
     4681 ttgaagaaaa agaatctggt tataaagtct atataggagg gaaatgggga aaatcagttc
     4741 gtcctggaac acagattgat agattatttt caaaagagga acttatgact ttaattgaaa
     4801 aagcaattct tttatttaga gaacagggta acacaggtga acgttttgga ataactatcg
     4861 atagaatcgg tgctgataaa tttatagaaa tgcttttatc agatgaagtt ttggaaagaa
     4921 aagaagagat actagaggct cttttacacc ttacaggtgg agctgtatgc taacaataaa
     4981 tattttaatt gtacagataa cctatggaaa aaattttagt taagaataat taaaattcta
     5041 tatatgatat atgataaatg aggctatctc aaaacacaga tacgcctcct tttcattgtt
     5101 gaaatgataa agtatatttg agttcatata aaaaagagtg tctcgtgaat tataaaattc
     5161 attttgaggc acccacatta ttttataata attgcttatt aattttattt taactatata
     5221 aaatgtacaa aaagtaagaa tatccaatat tctaaaatta attttgaatt gtattgattg
     5281 aaaagtttac tattccacta tttccgtctt gatttactac tagataccat gttccagtat
     5341 taggaacttt cattctacat ggatttttct ttgcaaatcc accatagtaa tcataatttc
     5401 tttgattttt aaatttcaca taatttatat ggtctaacaa tattacattg gcttgtttct
     5461 ctaaatttac ctctacaata tccccttttt ttaagttcat cttactataa ctatattcca
     5521 ttgaattact cctccagtat cgaataatct ttgttattta ctcatctcaa atatgtaaaa
     5581 attcatttaa aatattacaa atactatata attagactat tatccagttt taaccttatt
     5641 tactacaatc caaaggttat taaaaatatt attggcaaca acttctccat cccatacaac
     5701 tctttcttct ttattttcta ataaagcata gtacccagag tcagaacttt catctttata
     5761 gactgtaaca taaaactctt catcactata ttcaggtata tttttaaaat ct
//
LOCUS       DAELJO010000216         5809 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELJO010000216 DAELJO010000000
VERSION     DAELJO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.218"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            551..820
                     /locus_tag="KPB88_003393"
     CDS             551..820
                     /locus_tag="KPB88_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0055631.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1038..1283)
                     /locus_tag="KPB88_003394"
     CDS             complement(1038..1283)
                     /locus_tag="KPB88_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG0055632.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1709..2407
                     /gene="tcdC"
                     /locus_tag="KPB88_003395"
     CDS             1709..2407
                     /gene="tcdC"
                     /locus_tag="KPB88_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG0055633.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            complement(2742..>5809)
                     /gene="tcdA"
                     /locus_tag="KPB88_003396"
                     /gene_synonym="toxA"
     CDS             complement(2742..>5809)
                     /gene="tcdA"
                     /locus_tag="KPB88_003396"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0055634.1"
                     /translation="IAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSS
                     SFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKDIKKLSLG
                     YIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFYFDPIEFN
                     LVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAP
                     ANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNPNNAIAAV
                     GLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDC
                     VVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDDNSKAVTGWQ
                     TIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIA
                     STGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTL
                     NGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGY
                     ITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTYNNNIEGQAIVYQNKFLTLNG
                     KKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAATGWQ
                     TIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTHNN
                     NIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTIN
                     GKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIE
                     GQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKN
                     FYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSK
                     AVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         2053 a          791 c          791 g         2174 t
ORIGIN      
        1 caaatatatt gaatgaatat ttgactcttg gaatgtgtaa ttaaattaag tttggttatt
       61 cgatggaagg caaatttgat ataaaactcc atttaaagtg ccagatagaa actttatggt
      121 tatataaaag ttaaaagtaa tttatagtgt tatagaacat tcaaagggat ttaaagtaat
      181 ctatggtgtt ttgaaatatt caaagggatt taaactaatc tatagtcttg tagaatattc
      241 aaagagattt aaaacaatct ataatgttgt aaaatatatt caaagggatt taaactaatc
      301 tatagtcttg tagaatattc aaagagattt aaagcaatct ataatgctgt aaaatatatt
      361 caaggggatt tgaagtaatt tattgtgttt tgaaatattc aaaaagattt ttggaatagt
      421 ttttataaat ttaccagtac taataacttt atgtttaaca tttagatgac ctaaaatcaa
      481 ctatttgcac ttttgtattc atctaaaatc attttataat atttttaaat aatggaaagg
      541 atgtttgaac atgtttgaat caagatgtgg tgtctgttgt gacagttgta caaaaaaaga
      601 acaagtaaat tgcactggtt gcccaactat ggaaaaacca ttttggggtg gagaatgtaa
      661 ggttaaaact tgctgtgaaa gtaaggaact taatcattgt ggtgaatgtg atactttccc
      721 ttgtgatatg cttttaaata agggaaaaga ccaaggtttt gaccctatgg taaatattga
      781 gcaatgtaga aaatggcttg aagaaacagt ttctaattaa ttgttttaga atgctttgtt
      841 aagtaattta tttaatcctt tgttaggtag tatgtttagt catttgttga ttggtatatt
      901 tatacattta ttgatgggta tatttagcca tacaaataga tttacataaa atttacgact
      961 aaaaaagagt tgagtattag tttttattat ttaaaacgga tattcaactc ttttttatgt
     1021 ctaacttttt tgatttttta atctttccaa taccaccact ttaatttttc tggttctgga
     1081 ttttcagttg atgcaaaata tactgcacat ctgtatacat ataactgaca tctgtctatg
     1141 tgctgtccac gcattaaaca ttccttttca tacatttgtg ctgggtttgc atctttaagt
     1201 gatttaatag ttgtatatcc taacatgatt aaagattttt ctgttgcttt ccctacccct
     1261 ggaatttttt gtagttctcc catacttcac cttctttctg gtatattctt tttatattat
     1321 acctattacc agatattttt ttattatagt taatatttaa tatttaattt tttattatat
     1381 caatttattt atgctctttc atatatatat attttaccac ctctaaatca ctgagtcact
     1441 taattacatc ataatatagt tttatacaaa taaaatactt tatgtttaat ttaatatata
     1501 aaattcacct tcaagaaaat tatattataa tctgacattt ttatcttatt ttttcaaaat
     1561 atattgaatc ttcttacttt attttgtaaa attatgctta ggggaaatat attttaggaa
     1621 aatatgaata tataattttt agtcaactag ttattttaag tttttaactt ttaaaataaa
     1681 atatatctaa taaaagggag attgtattat gttttctaaa aaaaatgagg gtaacgaatt
     1741 tagtaatgaa ggaaaaggaa gctctaagaa aataattaaa ttctttaaga gcacaaaggg
     1801 tattgctcta ctggcattta ttttaggcgt gttttttggc aatatatcct caccagcttg
     1861 ttctgaagac catgaggagg tcatttctaa ccaaacatca gttatagatt ctcaaaaaac
     1921 agaaatagaa actttaaata gcaaattgtc tgatgctgaa ccatggttca aaatgaaaga
     1981 cgacgaaaag aaagctattg aagctgaaaa tcaacgtaaa gctgaagaag ctaaaaaagc
     2041 tgaagaagct aaaaaggctg aagaacaacg caaaaaagaa gaagaggaga agaaaggata
     2101 tgatactggt attacttatg accaattagc tagaacacct gatgattata agtacaaaaa
     2161 ggtaaaattt gaaggtaagg ttattcaagt tattgaagat ggtgatgagg tgcaaataag
     2221 attagctgtg tctggaaatt atgataaggt tgtactatgt agttataaaa aatcaataac
     2281 tccttcaaga gtattagagg atgattacat aactataaga ggtataagtg ctggaactat
     2341 aacttatgaa tcaactatgg gtggaaatat aactatacca gggatagctg tagagaaaat
     2401 taattaatat tgttttgtat tatagttaat attttatatt atagtcaata tgtttaaaga
     2461 tgtttttata attgcaaata aacagttaca aggctctaaa ttagtttttg cttttagcat
     2521 attatctatt ttttatcaac tattaattat ttagtattaa tatttccata tatgaatttt
     2581 attataaaat agtcaagaat aatagattat taaatgatag aaaaatttta actaaaagtc
     2641 atgtattaca ataacacatg acttttaatt aaatctcaat atttattata taaaaataat
     2701 ttcttagtag cacaggaata attttttatc aaacatatat tttagccata tatcccaggg
     2761 gcttttactc catcaacacc aaagaaatat ataacaccat caatctcgaa aagtccacca
     2821 gctgcagcca tagcagtatc aggcataaag taatatactt taccattaat agtttgccat
     2881 ccagtaactg cttttgaatt attaccaaag taatatattt ttccaagtaa atgtaggaat
     2941 ctattttgat aacgtatagc ttgaccttct atattgttag catccgtatt agcaggtgca
     3001 aagtattcaa atccattaga ccctttaaac actcctatct gaggtaaacc atttctaaag
     3061 taaaaatttt tattatcaat agttttataa ccattcgcac ccatagctgt attaggctcg
     3121 aagtaatatc tattaccatc aatagttacc caaccagtag ccgcttttga attattacca
     3181 aaataatata tattgtcatg taaatatagg aatctatttt gataacgtat agcttgacct
     3241 tctatattgt tagcatctgt attagcaggt gcaaagtatt caaatccatc aggtccttta
     3301 aacactccta tctgcataat accatcagta ttaaaataaa aatgtttacc actaataatt
     3361 gtataaccag ttgaagctat agaagtgtta gtattaaagt agtatttttt accattaata
     3421 gtttgccatc cagtaactgc aacagcagtg ttagtattaa agtaatattt tttaccatca
     3481 atagttcgca atccggtaac tgcttttgag tcactaccaa agtaatattt tttaccattc
     3541 aaagttaaga atttattttg gtaaagtata gcttgacctt ctatgttatt attatgagta
     3601 ttagcaggtg caaagtattc aaatccatta ggtcctttaa acactcctat ctgcataata
     3661 ccatcagtat taaaataaaa atgtttacca ttaatacttg tataaccagt tgaggctatg
     3721 aaagtgttag tattaaagta atatttttta ccatcaatag tttgccatcc agtagctgct
     3781 tcagcagtgt taagattaaa gtaatatttt ttaccatcaa tagtttgcca tccagtagct
     3841 gcttcagcag tgttaagatt aaagtaatat tttttaccat caatggtttg ccatccagta
     3901 actgcttttg agtcattatc aaaataatat tttttgccat tcaaagttaa gaatttgttc
     3961 tggtaaacta tagcctgacc ttctatgtta ttattataag tattagcagg tgcaaaatac
     4021 tcaaatccat taggtccttt aaatactcct gttaccattt tagattcatt attagcatca
     4081 aaatagaaat tatttctttc aatagtaata tatccatttt gaagaattcc atcataacta
     4141 aagtaatact tgtcattatt tatagtgcat agatgaattg cagcaatagc attattagga
     4201 ttaaagtaat atttcttatt gttaataatt ctccatccag taactgcttt tgagtcacta
     4261 ccaaagtaat attttttacc attcaaagtt aagaattcat tttggtaaag tatagcttga
     4321 ccttctatgt tgttagcatc cgtattagca ggtgcaaaat attcaaatcc attaggtcct
     4381 ttaaacactc ctatctgcat aataccatca gtattaaaat aaaaatgttt accattaata
     4441 attgtataac cagttgaagc tatagcagtg ttagtattaa agtaatattt tttaccatca
     4501 atagtttgcc atccagtagc tgcttcagca gtgttagtat taaagtaata ttttttacca
     4561 tcaatagttt gccatccagt agctgcttca gcagtgttag tattaaagta atatttttta
     4621 ctatcaatag tttgccatcc ggtaactgct tttgagttat catcaaagta atatttttta
     4681 ccattcaaag ttaagaattt actttgataa actatagcct gaccttctat gttattatta
     4741 taagtattag caggtgcaaa atattcaaat ccattagagg tactaaacac acctattttc
     4801 actacacaat cactatcaaa ataaaagtgt ttaccatcaa tagttttata accattaaag
     4861 gcaatagcgg tatcagtatc aaagtagtat ctactaccat taacagtctg ccaacctttt
     4921 gagatgatag cagtgtcagg attgaaataa tacttattat tgtcaattac ttgcaatccg
     4981 actgcagcaa tagcattatt aggattaaag taatatttct cattgttaat aattctccat
     5041 ccagtgactg cttttgagtc attatcaaaa taatattttt tgccattcaa agttaagaat
     5101 ttactttgat aaactatagc ctgaccttct atgttattat tttgagtatt ggcaggtgca
     5161 aaatattcaa atccatcagg tcctttaaat actcccaact gcatcacacc atcattatta
     5221 aaataaaagt gtttaccatt aataatttta taactagtta aagctgctcc agtatttata
     5281 tcaaaataat attttttacc attgatagtt tgccatccag ttactaagtt aaattctata
     5341 ggatcaaaat agaataatga attatttata ttgattaatc ctttaactaa tttactatct
     5401 tcatcatagt aataagtttt attatctatt attttaaatc ctaaatgatc tctatctaat
     5461 tcattttcag aattaaatga tttaaaatta ctcattatat atcctaatga tagtttttta
     5521 atatctttaa aatctatact cattttatta aatgattgag tattagataa gatacctttg
     5581 attcttattt tttgtaatat ttttttatta ctttcttcta agtatctaac taaaataaag
     5641 tcacttcctt ctgtagacca tttatactca aaagaagaac tatctaaatt tatatttact
     5701 tttttgtgga atgtatttgg gttaagaact ataatctcag ggtagtactc atttgaataa
     5761 taattggtat taatatttat taaacttgta tataaatcag gtgctatca
//
LOCUS       DAELJO010000217         5796 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELJO010000217 DAELJO010000000
VERSION     DAELJO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.219"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            132..1244
                     /locus_tag="KPB88_003397"
     CDS             132..1244
                     /locus_tag="KPB88_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0055635.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1452..1928)
                     /locus_tag="KPB88_003398"
     CDS             complement(1452..1928)
                     /locus_tag="KPB88_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055636.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2127..3752)
                     /locus_tag="KPB88_003399"
     CDS             complement(2127..3752)
                     /locus_tag="KPB88_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055637.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4090..5109)
                     /locus_tag="KPB88_003400"
     CDS             complement(4090..5109)
                     /locus_tag="KPB88_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG0055638.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5106..5675)
                     /gene="mocA"
                     /locus_tag="KPB88_003401"
     CDS             complement(5106..5675)
                     /gene="mocA"
                     /locus_tag="KPB88_003401"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG0055639.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1978 a          976 c          596 g         2246 t
ORIGIN      
        1 ttgacataat atatacattt tttaaataaa tatatacaat gtattaaaat gtatgcaact
       61 ctatttcaaa attattttgt gatatatata tgttataata aatgtataaa attttgattg
      121 gtgggttata tatgaatgca tttattagaa aaagaaataa aaactatgtt gtttacctgg
      181 aatttgaaga tgaaaagaca ggtaaaagaa aacaaaaaaa tatgggtact tttgataaaa
      241 aaagggaagc aagtaaaaga ctaaatgagg taaaagaaag cttatataat gatggttttt
      301 tagtaccaag cgaaattaca gtgtcagaat ttctattaga ttttctaaat aaatatagtg
      361 aaaatatatc tttagctaca tataataatt atgttagtat ttgtaagaat tacattaatc
      421 catctattgg aaaatataaa atacaagact tacatccggt tcacatacaa aactacatag
      481 ataaactatc ttataaacta aatcctcaat caattaagat tcatataaat atattgaaac
      541 ttgcaataaa aagagcttat agactcaaat taataaaaga aaatgttatg gatagtatag
      601 aggctccaag atataaaaaa tttaaaaatg agatttatga tagagaacaa atgataaaat
      661 tattagattt agcaaaaaat acagaaatgg aattacctat taatttggct attggattag
      721 gtcttagaat ttcagaagtt ttaggtctaa cttgggataa tattgatttt gaagaaaata
      781 caataacagt aaataagata acttcccgaa ttaatggttc tgttattctc aaagagccaa
      841 agacagaaag ctctgttaga aaaatatctt ctcccaaaga attaatgtct ttattaaaag
      901 aatataaaat aaaacagaat aaaaatttat taaaatcttc cattagaaat aataataatt
      961 tgttgttttt caataagaaa tgtgaaccta ttgctgaaga tgttatgagt aaaaaattta
     1021 aaagattttt agaaaagaat gaattacctc atattagatt ccatgattta agacactcac
     1081 atgttacttt acttataaat tctaaagtac ctataaaggt tatatccgag agagtaggac
     1141 actcaaatat aagtactact ctaagcgttt attctcatgt acttaaagaa atggataaag
     1201 aggcttctga caaaatttct gaaagtttat ttaatgctaa ttaatattaa tttatcttag
     1261 ctttaaatta gcttagctta ttgagataga ttcattaata ttaaatgtat ctatctcaaa
     1321 ttaataacta cttaaataat ctgttttatt ttaatttatc gcctcagtta ttatactttt
     1381 ttacgaaaaa ttatacttta caataaaaaa gatattccct tatagaaaat atctttttaa
     1441 atactatatt attatatact tgctaacatt ggaatatcta acatcttctt cattacatca
     1501 tctacttttt ttgctttata ttcattgtat ccaattaaat atcctttttc atatgaactt
     1561 tttaaaatct ctttttcata taaattatca gatttggctt ctgtgacact taaattataa
     1621 agccctttac acccatcttc ataaccttta ttataccctt tttctgcaaa atagttttta
     1681 tgattttttt cgttatgtac tgaagatatt gttactccag aattttcttt tgtactagca
     1741 taaggacaaa aacctttatt gtgttgatgt gctgaatgtc ctccacaata ataacgtgtt
     1801 aactctgatt tatttttatt atgatgacct cctgatgaat tatgccctcc tgagtgagca
     1861 tatgaaaaat tccctacact aatcatagct aaactaatta ctacagctac taaccttttc
     1921 ttaaccataa tctattcccc ccaaaatgtt atatattttt attatatcat ctactatatt
     1981 atatttaaat tcttaatata caaaatatta ctctattctg ctaataaaca tgtttaaaca
     2041 acataaaaaa cctcctaata tttgattaaa aatttacaca ctcatgcata gcatgtctca
     2101 aatattaaaa ggttttatac taataattat ttatcttttc catacatttt agctaattgg
     2161 tcttgtatct ctttactttc taaatactca tcaaagtcca tttttctatc cattattcca
     2221 tttggagtta tctctataat tctatttgct atagtagata taaattcatg gtcatgagaa
     2281 gtaaataaaa gcactcctgg gaacttttca agtcctttat ttacagaagt tatactttct
     2341 aggtctaagt ggttggtagg gtcatctaat acaagtacat ttgcatttga aagcattagc
     2401 ttactaagca tacatctaac cttttctcct ccagatataa cttgagcttc ttttaatgct
     2461 tcttcacctg agaataacat tctacctaaa aatcctctta taaaactttc acttttttct
     2521 tcagagaact gtctaagcca gtcaaccaaa gaatattcta ctccgtcaaa aaacttatta
     2581 tgattttttg gaagataatc ttgagaagta gttactcccc atttatactc tccactgtct
     2641 ggttcaagtt ctcccataac tatgtttaaa agtgcagtag tagctatttc atctcccata
     2701 aatactactt tatcatctct atccaatcta aatgacacat tatcaagaac cttaacccca
     2761 tctatagttt tagttaaatt gtgtacttct aaaacttcat tcccaatttc tcttgcaggt
     2821 gtaaatccta catatggata tcttcttctt gatggttgta tatcttcaac ttctaatttc
     2881 tctaactgtt tctttcttga tgttgcttgt ttagcctttg aagcattaga actaaatcta
     2941 gctataaatt cctttaattg agctatcttt tcttcagtct ttttattttg gtccttagcc
     3001 agttgtaaag ccaattgact tgactcatac cagaaatcat agtttccaac gtacatttgt
     3061 attttaccaa aatctacgtc aactatatta gtacatattt gatttaggaa atgtctatca
     3121 tgtgatacta ttataactat agattcttct aaatccatta taaaattatt taaccagttt
     3181 attgatttaa aatctaagtg gtttgtaggt tcgtccatta atagtatttc aggcttacca
     3241 aaaagtgatt gtgctaacaa taccttaacc ttttccccac caactagttc cttcatttgt
     3301 ttgtaatgca tatctttcgt tatgccaaga cccataagta ttttctcagc attagtttct
     3361 gcatcccatc cgtcaagctc tgcaaactca ccttcaagtt cggcagcttt tatcccatct
     3421 tcttcattaa agtcttcctt catatacaat gcatcttttt ctttcattat gttccaaagt
     3481 ctctcatgac ctcttataac aacatttaaa acagtttctt cttcatattc aaagtggtcc
     3541 tgttttaaaa ctgacattct ttctttatca gttatagata tactacctgt attaggctct
     3601 atttctcctg ataatatttt taaaaaagta gattttcccg caccattagc tcctattatt
     3661 ccatagcaat tacctttagt gaattttaag ttaacatctt taaatagttc cttatcacca
     3721 aatctaagtc caacacctgt aacttgtaac attaatatat tttcctttct ttttgctcat
     3781 aatttatttt atcataaaac tttgataaca tttttattat atattatcac gtaaatagta
     3841 tattttttat actaaatttg tgaaatattt attaaaataa tactaaaaat tgtaaattta
     3901 taaaaattaa caatctctta tattatactt ataaagaaat caaattgcaa ctttattcat
     3961 tcttttacac attgaaaata agttattata atttctaaat aaatatgctt tttttaaaac
     4021 atcattctag ctgttttgtt tcatatttaa aaagagtact taatttgtaa atactctttt
     4081 ttaataactt tatattttag caaaattaca tgcaggataa gtacatacat cacagttcat
     4141 acaaaggcca ccatgaccat atctagctat atcatactta tcaagctttt catcaattaa
     4201 aaccctagat aaaactatat caaatactgt agttttgtga tacattgcgc atcctggtat
     4261 acccataatt ggaacttctc cgtaataagc aagtaaaaac atagctcctg gtaatattgg
     4321 tgaaccatat gtcactaaat cagctccagt ttcttttata gctgttggcg taacatcatc
     4381 tgggtctaca ctcatcccac cagtacaaca gataagttct gctccttgac ttataaattc
     4441 ttttattgca ctctttataa tttctttatc atctgggcat atagtctgtc ctataacttc
     4501 acaaccaaag ccttttactt tttcttctat tactggtcca aatttatcaa caattctgct
     4561 ataaaacact tcatttcctg tagtaacaat tcctactttc ttaggtttaa aaggtactac
     4621 atttactatt tttctattac caactacttt ttttgcatct tctaattttt tctcatctat
     4681 aaccaaaggt atcactcttg ttccagctac ttttaaacct ttatttacta taaaattatt
     4741 atgaagagta gccatcatta tttctccaac acaattcaaa tcaaaaagtg catcaacatc
     4801 tatctttaat agaccatcac atgctgctat aaaatctatt ttcccttctt ttatttcact
     4861 aaaatctaaa ttttctccag cagttagttc ttttaaaaac aatgcgcctt cattttcatg
     4921 aaccattcct tctgactttt cccatacata caaattatct ttaccaagag ataataacac
     4981 tggaatatct tcttctttta ctatgtggcc ctttttaaat tttcttcctt taaattctcc
     5041 aggtattatt tgagttatgt cgtgacataa aacttgtcca acagcatcta tagtttttat
     5101 tagcttcata atttaccccc aattctctca tagtccattt ttgtatcgat atcaaataac
     5161 atttctttag gaacttctac atactttatt tttgatgaat tttttaaaat ggttttacct
     5221 ttttcatctt ctttaagtgc caacaattct tctttcttgc tatatgggaa tatgactgga
     5281 tttccacaca tatctttgta tcttggaatt actataaaat ctctatctaa tttaaaagta
     5341 tttataattt tatctatata taagttatca ataaatggct ggtctcctac aaagaacata
     5401 taaccatcac attttgagct atttaaaact cctaatttta tagattcact ttgacctata
     5461 tttgcatttt tattatctat atattcaaaa ccatatttat tacacaataa caaaacctct
     5521 tcatattgac ttactactac aacttgatga aaatcaatct tgctaacttc tctaaataca
     5581 tgttctatta tagaacagcc tttataattt aataaaagtt tatttgctcc cattcttctg
     5641 gcaaagcctg atgccattat cactgcattt atcatatata tcatccttgg tatttataat
     5701 atattgatag ttctatgatt tttataatat ctctattgat attatctata tatataatat
     5761 catataaact attttaatac ttcaatatct tacaca
//
LOCUS       DAELJO010000218         5776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.220, whole genome shotgun sequence.
ACCESSION   DAELJO010000218 DAELJO010000000
VERSION     DAELJO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.220"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(246..623)
                     /locus_tag="KPB88_003402"
     CDS             complement(246..623)
                     /locus_tag="KPB88_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0055640.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(810..1781)
                     /locus_tag="KPB88_003403"
     CDS             complement(810..1781)
                     /locus_tag="KPB88_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0055641.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFITTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1815..2012)
                     /locus_tag="KPB88_003404"
     CDS             complement(1815..2012)
                     /locus_tag="KPB88_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG0055642.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(2080..3291)
                     /locus_tag="KPB88_003405"
     CDS             complement(2080..3291)
                     /locus_tag="KPB88_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG0055643.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            3572..4174
                     /locus_tag="KPB88_003406"
     CDS             3572..4174
                     /locus_tag="KPB88_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0055644.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(4430..5401)
                     /locus_tag="KPB88_003407"
     CDS             complement(4430..5401)
                     /locus_tag="KPB88_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG0055645.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(5427..>5776)
                     /locus_tag="KPB88_003408"
     CDS             complement(5427..>5776)
                     /locus_tag="KPB88_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HBG0055646.1"
                     /translation="DSNKFGLELFSSQDEGVKLEFNKLGNIVTLDRSNFKKVFGVEYG
                     TNRKEYINIDENTNIKVLADRSILEIFINEGEVVFTSRIFAKENSNQIRVYSDKIVEY
                     EYTKFKLKQGIEL"
BASE COUNT         1994 a          922 c          632 g         2228 t
ORIGIN      
        1 ataatacttt agattaatga atattgacaa gttataaaat tattttaaat tttaaaaatc
       61 atttcttatt taaataagtg gtatcattta acttgatact ccctattcca attattttag
      121 gcattttact aattagagaa cctatttaat attactttat gaatagtgag tagctgatta
      181 aataatacca tttatggttt tcagccatag attaattaaa ttcttagagt tccatcctga
      241 tataattact cttcttctaa atttttaaaa tgactttcaa ataaatccaa catatcttta
      301 tcaacaactt catcatcatg tagtgctgaa attaaactct gtaaagaatt gtcgtgtata
      361 ttgcttaaaa aatcttttgt ctcaacactt aaatactcct tatgtccaac aactattgta
      421 taatgagtgt atctatctat tttttctgca cttaaaaaat gttttttaac aagtcttgat
      481 aaaagtgtca atgtagttgt ttgtttccaa ccatattttc gttccatagc ttcaatagtt
      541 tcttttgatg ttactgtatc atttttttcc caaataaact tcataacttt taattctgct
      601 tgtggtattt ttgaaattgt cataacctta cgcccctctc aaacttttat taaattaata
      661 ctatcgcaat atagttttta agtcaaagat tttgtcgata ataatatttt ccaattttta
      721 ccatagggtt atcttttata taattctatg ataaattaca taaaaaatat ttattttaca
      781 tcataaaatt agaaatgtaa atagtatgat tatttagtct tatctttaat aaacaatcca
      841 gtaaatttat acatccaaag tgcattcata aacaacaata aacttgttga aaaaaatact
      901 gttctaatac ctaaatgtgc tgatatttgt ccccctaaaa atgctcctga tgaacagcct
      961 atatactggg cagatgcatt atatccaaat atttttccca catattcttc tggtgcaatt
     1021 tttttaagca agctgttaac agaaggatta agccctgcaa ctgacaaccc taataaaaat
     1081 ctcaacaaca tcagttgcca tgcagttttt acaaaagctt gtggtataaa tataatccca
     1141 gcccataaaa gtgaaattaa caatactttt tgaggaccta ttttatccga catttttcca
     1201 agtttttgag ctgctaataa atttgctaat cctgatgcag aaaatgtcaa tccagctatt
     1261 aaagctacat ttgatgcaaa atttgtaagt tgtgatatat atatggttac aattggttct
     1321 atagagtata aagctagttg agtaatgaat gtggttataa aaatagatgc aattaaaaat
     1381 ttatttggca atatatttaa ttgttcttta ggtatagaaa ttgattcttt tatttcttta
     1441 ggtttaaaat tatctactac aaataaaata gagatgataa atgatacaaa caacaatcct
     1501 cctgttataa taaatgtact tttaagacct aagattgatt ctatatatcc tcctactgtt
     1561 ggaccaatta atgagccagt agttgatgca gttgcaagag tacccaaagc ccatcctgaa
     1621 tgatttttat ctgtctgtgt tgcaataaga gtagtacatg cagtagcata acccgtaaaa
     1681 actccttgaa gtattcttaa tcctaataac tgatagacat tttgaacaaa cgccatggta
     1741 aatataacta ttcccatccc aatacttgca cgtaaaagca ttgattttct tccatattta
     1801 tcagcagctt tccctcaaat tggcgaaaag aatgctgcta ctacaaaggt acatccaaaa
     1861 attatacctg aatactgttg tattaaacta acatcagtta cccccaactg tttaatatat
     1921 aatggaagta taggtgctat ttggctcatc cctatagaac taaaaaacat acctatccaa
     1981 catacaaata aatttctttt ccacaattcc atatagaatt acaccccctg cattttaaat
     2041 tttttcaata atactaagtt tattaattta tgctattgtt tactcaatat atccatcttt
     2101 tcttttataa tattgtttag gaaattcata acttgaaaat aatagcggtg ataaagaata
     2161 ggagtaactt ccagcattac taattgaaat caaatctcct atgtttgctt ttttcaattt
     2221 aacattattt gcaattacat ctaatgatgt acagagattc ccaacaacat ttacatactc
     2281 ttcttctttt aaattattta atactgaaag agaaaaggca tgatttgatg tatataatgg
     2341 ctcttgacct tctaattcta ctccacaagt aatattttca attaaattag aaataatagg
     2401 tctcataaat ccattaagtc cattttttac aataagatat tttttccctc ttgatacttt
     2461 aatatctaca attttaacat aataattacc tgatttacaa actatgaaac gtcctgattc
     2521 aataagaagt cttgatttta gtgtgtttga attagtaaaa gcaatctttt gagttaattt
     2581 tgaaagctct ttaagattaa gtggattttc tctaagatta tcataagcta ctccaagtcc
     2641 actaccaaaa tttataaatt caatattaat atacttactt ttatttaaat ttaaagctat
     2701 aaaaaaacat ttttcataat aatgtcctaa cttttcaaaa tccaatacct gagatttaat
     2761 atgtatatga atcccaacta cagatacata atcacacata cttaacatct taaaaacact
     2821 gctcatatct tctatatcta taccaaattt gcttgattta gcactatcat catccatagt
     2881 aaaatcagga tttattctta ttcctatttt aactttctga ttatactttt tagatatctt
     2941 atttataagt tctatttctg taatactatc tgcaataatt atgcatttat ctaaaacagt
     3001 ttctatatca atctctgttt tgcctggtga tgaataataa atatcatctt tagaaactcc
     3061 acattctgat gaaatcataa cttcattagt tgaagaagca tctgctccaa aaccttgctc
     3121 tgcaatgctt tttacaattt tgttatatgg atttgttttt atagaatata aaaaatcaaa
     3181 acctaaaata ttatttttca attccttaca tttttcaatt atattagtct catcatatat
     3241 ataacatggt gcattttctt ctataaattt atagtcttca tttgagtaca tattttttac
     3301 ctccaagatt ttaaatataa tcataaatta agcaattata atcctataag ataattttac
     3361 aataaattac catagttaac tacacacata tatgtaaaaa catacctaaa atatttttaa
     3421 ttatagatac attccaactt ttattgataa aacaatttgt ttcaatttta ttacaaatat
     3481 acaaagtgta ttgttgttaa cgtattctca tagtatccta aaactatatt aagcactaaa
     3541 aactatttta aaatactgtg gaggtatcaa tatggaagta tgcattctta tgggaagtcc
     3601 taagaaaaaa ggtaatactg ctgctatttt aaaacctttt attgaggaat tagaaaatta
     3661 taattcaaat attgaagtaa tatggttata tgattgtaaa atagagccat gtattgcttg
     3721 taaaaaatgt caggataact tatttgattt tggatgttat tgtaaagatg atgttcagaa
     3781 aattttcaag aaaattctta tgtgtgattt aattgttttt gctactccaa tttattcttg
     3841 gtactgcact tctccaatga aagcactact tgacagattg gtgtatggaa tgaataaata
     3901 ctatggtaat gagaaaggcc ccgctctatg ggcaggaaaa ccagtagcac ttattacaac
     3961 atgtggatat agacctgaaa agggtgctga tatttgggaa aatggtatga agcgctactg
     4021 taagcattca cagttaaaat atataggaat gcttgttgaa cgtgatttag gtaaacctaa
     4081 ttttatgaat aaagaaaaag aagaacattc tcgtcaattt gcaaaacaaa tttattatat
     4141 tttagataat tcaattgaag tagataactg ctaaagacat aatactccaa gtaaagtatt
     4201 gataatataa ataaatctta cataatttgc ttatttaaaa taagatatat attcttctaa
     4261 aaagtaacaa taattaaaat attttttatc atttcaacaa tgataaagag gcttatctct
     4321 attttgagac agcctcttta ttgcttttta tatgttaatt attttcatag taaacatcct
     4381 taaaaactct ttacatgtta cttccttcaa aacctattac tcattcctac tataaatttt
     4441 ttataaattc agttatttct tcctttgttg catatgaact tatcgcacct ttaatagttg
     4501 tactacgagc tgcataataa ttagaaaatt ctaaatactc atttaacgtt tcttgtttca
     4561 tttcttttat atcattcaat gttatttgtt tatacaataa ggtgtataaa aatgaaccta
     4621 tataagaatc tcctgctccg gttgtatcta ttgcatttac tttaacccct ttacacatac
     4681 cttttacttt tttagtgtat gcatctactc catcttttcc ttttgtatat aatactagtt
     4741 caacattccc cacaaagagt ttatgtaatg atttttctat atcattttct cctgttataa
     4801 attctaattc ttcatcagat atcttaacaa tatgagcaaa aggtaaaaat tctgatattg
     4861 cctttttaca agattgctca ctatcccata aaggtaatct tatattaggg tcaaagctta
     4921 ttatactatt actttcaagt gcgtattcta ttgctttttt atgtgctaat ttcattggac
     4981 tatctattaa atctactgaa caaaaatgta ggatatgaca attattaaac cactcttttt
     5041 taacttcatc agcttctaaa agcatatctg cacttggatt tctataaaat gagaaatcac
     5101 gattaccatc ttcttttaat gatacaaaag caagtcctgt atttgcttta cttgttctca
     5161 atacataatc agtatttaca tttacttcat ttaatacatc tattatataa tcaccaaatg
     5221 catcttttcc taacttagat ataaaactcg attttcctcc aagctttgct acaacagcac
     5281 taacatttgc tggagctcct cctgctactc ttctaaattc tgatacattt tttaatttac
     5341 caccattttg attcggtata aagtctatta aagcttctcc tatgctaatt acttttttca
     5401 tcttttctac cccttataat gtgtttttat aattcaatac cttgctttaa tttaaattta
     5461 gtatactcat actctactat tttatcacta taaaccctta tttgattaga attttcttta
     5521 gcaaatattc tactagtaaa tactacttct ccttcattta taaaaatttc taaaatactt
     5581 ctatccgcta gtactttgat attagtattt tcatcaatat ttatatattc ttttctattt
     5641 gttccatatt caacaccgaa aactttttta aagttgcttc tgtctaaagt tacaatgttt
     5701 cctaatttgt taaattctaa ttttacacct tcatcttgtg atgaaaataa ttccagccca
     5761 aatttattgc tgtcaa
//
LOCUS       DAELJO010000219         5704 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELJO010000219 DAELJO010000000
VERSION     DAELJO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.221"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1119
                     /locus_tag="KPB88_003409"
     CDS             <1..1119
                     /locus_tag="KPB88_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0055647.1"
                     /translation="KAINDIYGHKKGNLILKDIADTLNELTNINETFARVSADNFNIL
                     LTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSIKNNSD
                     VNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEALVRWQ
                     YTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVNFSRVD
                     FFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFIVAMDD
                     FGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRVVAEGI
                     ETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1152..4589
                     /locus_tag="KPB88_003410"
     CDS             1152..4589
                     /locus_tag="KPB88_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0055648.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4607..5353
                     /locus_tag="KPB88_003411"
     CDS             4607..5353
                     /locus_tag="KPB88_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG0055649.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEKHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5302..5610)
                     /locus_tag="KPB88_003412"
     CDS             complement(5302..5610)
                     /locus_tag="KPB88_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0055650.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2381 a          567 c          872 g         1884 t
ORIGIN      
        1 aaagcaatta atgatatata tggccataaa aagggaaatt taatattaaa agatattgca
       61 gatactttaa atgaattgac taatatcaat gaaacttttg ctcgtgtaag tgctgataat
      121 tttaatatat tattaacata taacaaaaaa gaggatataa taaatattat caaaaagatt
      181 atggcaaata atgaacttgt aaacttatct tttggaattt atgaaatcaa agataaggat
      241 ttatctgtaa gtgtatatag tgatagagct tcacttgcaa aatcatcaat aaaaaataat
      301 agtgatgtaa attttgcatt ttttaatgat aaattaagag agaaattatt acttgaagat
      361 aagatagaaa aagaaatgga atatgctctt gaaagtgggc aattcgtaat gtatttacaa
      421 ccaaaataca atattaaatt agataaattt tgtggaagtg aagccctagt cagatggcaa
      481 tatacagaaa aagaggtcat atatccaggt gattttattc ctatatttga aaaaaatgga
      541 tttattagaa agattgatat gtacatatta gaacaagctt gtaaagaaat ccggtcttta
      601 tttgataaag gaatttcacc tttacctata tctgtaaact tctcaagagt tgattttttt
      661 aaaaaagatt ttattgaaaa catagtaaat atatgtgata gatataaaat accatatagt
      721 ttaatagaaa ttgaaattac tgaatctagt atgtttggag atactgatac tctatttaat
      781 gttagtagaa atctacagga tattggcttt atagttgcaa tggatgattt tggctcggga
      841 tattcttctg taaatatgct taaaaatata ccattaaatg taataaaatt agatagagga
      901 ttttttgttg atgataaaga tgttgataag agtcaaattg ttataaaaag catagtttct
      961 ctcattaagc aattaggaat aagggttgtt gctgaaggaa ttgaaacaag aagtcaaata
     1021 gaaatgttaa aaaaagcaaa ttgtgatatt gttcaaggat attatttttc aaaaccatta
     1081 ccaattaaag aatttgaaaa attagtctat aaaatatagt cagcgagtct taaatgggag
     1141 gtggtgatta agtgataaga gcaaaatcgt taaaaaatgt actgagtatt gttttaatat
     1201 ttttatttac aataatttct ttttctgatt ttgcatttaa aactaatgtc caggaagaag
     1261 aaaatagtaa aattaattta agggaaatat ccctacaaag ttccaatgct ataaaaataa
     1321 aattagatag ttctctatcc tttattaaaa gtgtggcaaa tatggtttct tattttaata
     1381 ctatagataa ctcaaaaata gttccattat tagctaatat atcaaaagaa tctgactttc
     1441 aaagaatctc tattgtatct cctgatggaa taagtcatac cagtgatggc aaaattatca
     1501 atgtgtcaaa gtatcagtat tttaagaaag ctattaaagg agaaagtaat atatctgaag
     1561 ttgtgaaatc tccaattgat ggaaaagata cttttgttat agctactcct gtatttaata
     1621 agaataacaa aataattggt gttttatacg gttcttacta caatgataaa ttagctgaat
     1681 ttatagatat aacaagtttt aatagtgaag ggtatataga tatatttgaa tctagtggtc
     1741 aatttgtttt aaaatcttca catacaaaca aactgattaa gtcaagtagt aacatatatg
     1801 atatatttaa aaaagcttct tttgatgatg gttatagcta taaaaaaata tatgaagata
     1861 caaaaaataa caagagtggc tttacctcat atgaatacaa aggagaaaat cgttatgcta
     1921 cttatacacc cattggtgta aatgactggt atgtaatttc tgtgatacca caatatactg
     1981 taagaagaaa cattgataaa ataaatcaat tagcattttc tcttattttc aaggtattct
     2041 tattgtttat tatgcttata atatatatta tttataaaaa caataaatct cagaaagata
     2101 tactaaaagc taacaaagaa atctcatcta ttacaaatag cattcctgga ggagtacaaa
     2161 agtgtaccct gggtgaaaca tgcaatatta attttttaag tgatggattt atagagttgt
     2221 ttggttatac aagagaagaa ataaaaacat tgtttaataa tgatttttat agtttaatac
     2281 ataaagatga tgttgaaaga ataaaaaaag aaatttttaa acaactttac aaaaataaaa
     2341 ctattgaatt agaatatagg attataacaa aaaatggtaa tacagtatgg gttttagata
     2401 aaggggagct tataacagaa cctgatggaa aagaatattt ttgttgtgta actgtagata
     2461 ttactagaac aaaacaatta gaacaagaga tggaacaaaa aaatctagaa cttgaaatga
     2521 tatcagaaaa tgttttaggt ggtataatta taacagaatt taatgatgat tttactataa
     2581 aatacgctaa ctctggttat ctttcaatga ttggatatac acgtgaacaa ttagaaaatg
     2641 aacttaaata caaagcttgt aatcttgttt tttatgagga taaaaagaag gctcttgaaa
     2701 gtatacaaca tcaattaaac acaaaaggaa tagctgtata tcaacataga ataagaagaa
     2761 gaaatggtaa tattatttgg gtttctataa aaggaaggct tattgttgat gatgatggta
     2821 ttgaaaaagg tatttggatt ctaaatgata taacagattt aaaaaatgca gaacaaaaac
     2881 ttaaaataaa tgaagaaaga tttagaattg cattaaagca aacatcaaac actatatttg
     2941 agtatgatat aaaaacaaag tcacttttaa atactagtaa agagcttgat aaatataata
     3001 tttataaaat tactaaaaac gttccatatt ctctagtaga atcaggagtt attcataaag
     3061 attttagtga tgattttata aatatgtatg aaaatgtaat aaatggagca aaaaaagcaa
     3121 gttgtgtagt taagatgaaa cttccagatg aaaattatat atggaataaa gttaccttga
     3181 ctactatttt tgatgaagat ggtaatcctg taagagcaat tggtatacag gaagatataa
     3241 gcaaacaaaa ggaagcagag ataagataca atcaagaagg taagtatcat tcagctatat
     3301 tgtcggaagc aatgataact tatgaaataa atgtatcaaa ggatactttt atcaaaggaa
     3361 atgaaagttg gcaaaaacca tttggaatta agcctgacaa taactattct caaatggtaa
     3421 aaactatagc aaatagtatt gtacataaag acaataaaga aacattttta aatacatttt
     3481 tatgtgaaaa cttattggat gcatataaaa ataatgtgac tgaaataaaa ttagaatatc
     3541 gaagaatgaa tcaagaaaat aaaatgattt gggtcatttg tacaatgcac ttaataaaag
     3601 attctattac gaacgatata aaagcacttg cttatgttaa agatattgac aagcaaaaaa
     3661 atgaagaaat tagattaaaa ttaaaagctg aaagagatac tcttacagga ttatataata
     3721 aaggtactac agaaaaattg attaatgatt tcttgaactc tgaggaagca agcttaggaa
     3781 tgcatgcatt tttaattgtt gatatagata attttaaatc tgtgaacgat aatttaggac
     3841 atgtatttgg agataaagta ttatttgaag tttctaaaaa cctaaaacct atatttagaa
     3901 aagatgatat agtaggtaga attggtggag atgaatttat agtattctta aaaaatattc
     3961 agtcttttga gaatatcaaa aagaaagcag aaaaaatatg cgatatattt agaaattctt
     4021 atacagaaaa taaaaagaca tataaaatat ctggaacagt tggaatttca tttgtaccag
     4081 aacatggaac tgtatttgaa gaactttata aaaaagcaga catagcactt tattatgcaa
     4141 agagcaaagg aaaagataag tatgcacttt ataatgattg tatctaccaa aatgaacttg
     4201 aaaataatat aagtgataat aatataaaat tagcagataa atgtgagaaa tccaaaccag
     4261 tacttaaaga aattcttgat tctattgata attatatcta tatagtgaat ccaaatactt
     4321 atgaacttct ttttgtcaat aataaggtgt ctcgtttgga tttagagata aaattaggag
     4381 aaacctgtta taaatcacta ttaaaatctg atagaccatg taataattgc cctcttaacg
     4441 gattagaaga aaataagcat tcaaaattca aaatagagac aaatacattt ggtaaaaata
     4501 tcagtatagc aagttggatt aattgcatag atggagaata caactgtctt atagagtgta
     4561 tagattcttc tttatacaaa aataaataaa aaaggactat gaagatatgt taaatagtat
     4621 aactttgata cttggtataa tatttacaat ttactatttt tatctcaaaa tagtttttgg
     4681 aggaatttca tttagtgaga ttttcttagt tgtaggaatt gtactaatta tctatcaaat
     4741 atttaagaaa aaaattaaag aaaagaaggt tttttatagg gtgttaaaaa taataatcag
     4801 cattttttta atagtatttg tagttactga gagtctaatt attttttatc ctaaaaatag
     4861 tttagaatca aaaagtgatt atctcttaat tttaggagct tctgtaaaaa agactacacc
     4921 aagtacaacc ctgaagggta ggttagacac tgcattgaaa tacctaaaaa ttaatgataa
     4981 ttgttatgtg gtagtatctg gtggtaaagg aagtggagaa aaaataactg aggcaaaagc
     5041 tatgaaagat tatcttataa aaaatggtat agataaaaat aggataatag aagaggataa
     5101 atccacgaat acttatgaga attttaaata ttcaaaattg aagatagaaa aacatagtca
     5161 aggaaaacta agcgatttaa aagtaaaaat agtaacaacg gattttcatg ttctcagaag
     5221 taaaatatta gcatatagaa atggatataa aaatacgtct ttttatgcta gtaaatcaaa
     5281 actttcattt gtaccaactt actatacaag agaatttttt gcattatgga aaacaatcat
     5341 ttttgataga taataaataa aaagactaga tataaactca tatctagtct tttctattcc
     5401 cttaattcta atccattttc caactttact tgttgtagaa atttcaacat tgggatttac
     5461 aagtaattgc ttataagaag ttttatattt tacgattcca aaatataatt ttccttcata
     5521 atccattata aatccgaatg gtcttactct tggtttatta tcttcaactg tagcaaaata
     5581 gaatgttgag ttttctttaa aaaaattcat tattttttca tgattaaatc ctctttgtat
     5641 cttatactaa tataatgaat ataaataaaa tattcataat actaatataa atcgatataa
     5701 aaac
//
LOCUS       DAELJO010000220         5703 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELJO010000220 DAELJO010000000
VERSION     DAELJO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.222"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            286..957
                     /locus_tag="KPB88_003413"
     CDS             286..957
                     /locus_tag="KPB88_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055651.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEEHHIVTLIRSGYK
                     FEVKE"
     gene            954..2084
                     /locus_tag="KPB88_003414"
     CDS             954..2084
                     /locus_tag="KPB88_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055652.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            2529..3374
                     /locus_tag="KPB88_003415"
     CDS             2529..3374
                     /locus_tag="KPB88_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055653.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            3374..4402
                     /locus_tag="KPB88_003416"
     CDS             3374..4402
                     /locus_tag="KPB88_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055654.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            4405..5418
                     /locus_tag="KPB88_003417"
     CDS             4405..5418
                     /locus_tag="KPB88_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055655.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         2126 a          560 c         1000 g         2017 t
ORIGIN      
        1 atttattcat taattgaaga tatttgtttt agaatatatt ttaatcgaaa aaatagaata
       61 gtgacaaaat aatataatta ttattaaaaa tattttagcc tgctaacata gaaaaagtta
      121 gtaggctatt tttatctatg atatcaattc cattaagaat agtctaaaaa atactatcaa
      181 aatgctataa aaaatactat caaaatacta taaaaaaata gttgaaaaat aaataaatta
      241 atataaacta aaataaagcc tacaaaaaag aggtgataaa tagaaatggc taaaatacta
      301 attgtagaag atgaaaaaag gatgcaaagt attattgtag aatatatgca aaagggagga
      361 tatacttgta taactgctga tgatggagtc gaggcactta caatattaaa gagcaacaat
      421 atagatttaa tgatattgga tattatgatg ccgtatttag atggattttc agtgtgtaga
      481 gtttcacgtg aaatgactaa tatacctatt attatattga cagctaaagg tgaagaggag
      541 gacaaattaa agggttatga atatggcgca gatgattata taacaaaacc atttagccca
      601 aaagtacttt tagcaaaagt taatgccttg ttacgtagat acactactga tattcccaaa
      661 aattcattga gtttaggaaa gatttttatt atggtagcat caagacaagt gtatgtagaa
      721 gataaattga ttgatttaac atataaagaa tttgaattat tgcgtttatt tatggaaaac
      781 cctaatcaag ttttttccag agagaaactt ttaaattgta tatggggata tgattttgaa
      841 ggaaacacac gaactgtaga tacacatata aaaaccttac gcaaaaagct tggtagtgaa
      901 gaacaccata tagttacatt aattcgttca ggatataagt ttgaggttaa ggaatgaata
      961 gtttaagaaa gattatagaa aaactgaaaa atgtagatat ttcaaattgg attaaacaaa
     1021 attttggact atattcagtt agaaaaaaag tgtttttact ttctaaatta tcaggagcgt
     1081 taattatatt attttatctg gttgttgaag aattttcgat aaactctaaa ttagaatttt
     1141 ggatttggtt tataatactg gttatattta ttatgagtat tgatttctta ttgggaaaat
     1201 ttatttcaga accaatcaca tctataaata aatctgcaaa aagtatgtcc caacttgatt
     1261 tttctaatcc atgtactgta aatactaatg atgaatttgg agaactttct aggagcctga
     1321 atacaatgtc tacaaatcta caacaggcat tatcagacct agaaagtgca aatattcaac
     1381 ttgaaaagga tgtaaataag gaaagaatgc tattggaaca aagaaaggag ttggtagata
     1441 caatttcaca tgaaatgaag actccacttg gcattatacg tgcgtataca gaggggctta
     1501 tggatgaagt tgatgaagaa aaaaagaaaa attatatgaa tgttataata gaagaaactg
     1561 atagaatgaa caatatgatt gtatcattat tagacttatc tgcattggaa gcaggagttt
     1621 ctaaacttaa cccagagcgt tttgatttta tagagtttgt tgaaactgtg gcaggtcgtc
     1681 ttttaattga tataccagat gccaattttt attttacata tgatttgcca gagtatgaag
     1741 tttttgtagt ggcagataaa atgagaatgg aacaagtagt agagaatcta gttattaatg
     1801 caaagaagca tgttgtatat aatggtaatt tagatttatc gattacatgt gaaaatggct
     1861 tgcttttatt tgaaatttat aatgatggta ggtttataga gcaagatgag atttcaaaga
     1921 tttggtctaa gttttatcgt agtgttcaat ctcagcgtac aggaggttca ggtctagggc
     1981 ttgcaattgt ttcacaaata cttacaatgc aagggttaga atatggagtg aataatcgtg
     2041 atagaggagt agagttttat tttatgattc caattgatga ataatttagt attaatttat
     2101 aaattataag tatctgatgt aaaatatttt tatttgtata tgtaagcatt taaagagaat
     2161 aaaataaaaa aacttaatga aaaaagctta atgaaaaagt ctaataaaaa gtttaataaa
     2221 aagcttaatg aaaagcttaa taaaaagtct tgttttcctg atgaaaacaa gattttttat
     2281 taagcttttt attatttcac ttcgatttca caagagagac actctgattt caccaaatag
     2341 aaataaaata gggttaaata aattaaaaaa tagaagcgta attaaataag gttacaaggg
     2401 ggagtactta tgaaatggta tacatggttg actattatat tgataatatt gctttctatt
     2461 ccgcttaaaa ttaaatttat aaaatggtgg gatagacgac aaaaaaatga taaaggaggt
     2521 tatgatagat gataaaagta ggagagttaa aattttctta tggaaaagat aaacagatat
     2581 tgcatggact taattttgaa gtaaaagaag gagaaatctt tggattcctt ggacctaatg
     2641 gttcaggaaa atctacaaca cagaaaatct taaatggtgt tttaaaaggt tacaatgggc
     2701 acgtatccct ttttgggaag gaagttaaag catatacaga gtctctatat caaaagattg
     2761 gtgttttatt tgaatttcct tatttatata ccaatttaag tgcaattgat aatttagaat
     2821 atttttcgtc cttttatcct aaaaatcaaa ggagagacat atatgaactc ttagatttgt
     2881 tagagtttaa gaaagagttt ataaataagc ctgtatcatc ctactcaaaa ggtatgaagc
     2941 aacgtattag catggcaaga gcattggtaa gtaatccaaa gttgttattt ttagatgagc
     3001 ctactagtgg acttgaccca tctggagctg tactatttcg taagattatt gaagaagaac
     3061 gaaaaaaagg aacaactatc tttttgacta ctcataatat gctagatgca gatttaatgt
     3121 gtaacaatgt ggcttttatt gcagatggaa agattatggt aatagataaa cccaaaaatt
     3181 taaaaatgaa aaatagcaat aataaagttg aagtggaatt tgtatataat ggaaatcgag
     3241 atattgaaat tgtagatata caagagttag aatctggaat aacttttaaa tatgatgaaa
     3301 tcttaagtgt acattcaaaa gaacctacac tagaagaagt ttttattaaa tacacaggaa
     3361 ggatgttagt gtaatgtatt ctaaattttt taccttagta aaaaaagatt tttggatgat
     3421 gatttatggt aaattttttg tgctgacact tggctctctc atactttatt cgtgttatat
     3481 taattttggt tatgtaaagt ttatggatga agatagttat cacaataatg tatatctata
     3541 taatccagag aatataagca tgagtgcatc tccattaatt aatcaagttt cttctatagg
     3601 tgaattaaaa gcaaaacttg atgaagatac aagtgcagtt tgtattgata tgagttccag
     3661 taagccgaaa gtaattttac atgaaagtat taaaggagtt gacaattata gagctgacta
     3721 tgcattgtca ttattatcat cagaaaaaga ccataatagc aaaatatcag acaacaatgc
     3781 aataattcaa attaatgata aggatacatc tgagataaaa caaagaaaag acatgacttg
     3841 tgagatttta ttctttgaaa ttgtagctat tggattttta gggattgcct ctttattatt
     3901 taaagaaaag cagatgggag taatgcgtat tactggtatt ttacctgtaa aaagaagtct
     3961 ttttatatta tcaaagatat ttgctttttt tatatgtgac ctatgtttta cagttctttt
     4021 agtagtattt aatattggat ttttagaagg aataaaaata cttccacaag tattagttca
     4081 agctagtatt ttatcaatta ttatgtcttt gattggaata ggatgctcaa tgttattgag
     4141 agattttaag caattttctc ttatatatct tgtggttgct gtattcataa ctacacctat
     4201 atttctatct gcaaatacat cttttgaaat agattggatt aaatatcatc ccttctatca
     4261 tgtttatagt cagcttaaaa atgcgttttt catgaatttt tcaaaagaat tagtttattt
     4321 tgtagtgtgt tctttgacaa taatagttct atttttactt gtaaaatata tttttaataa
     4381 agagttggtt agggagggat aatcttgata ggattacgtt accagttaaa gagtataaaa
     4441 aaagataaaa tgtgtataat ttccttcttt ttaccagttg ttatggcgat tttaataaat
     4501 tttatgggaa caattgatat atcttcatta ggagagatac gttttggtat agtgaaggat
     4561 acacttcctg tacaaacaga gcagtggttg aagaagtatg gtatggtaag tgtatatgac
     4621 acaaaaatgc atttaatacg tgaaattaag gatattaaaa cggacacaat tggagttgtt
     4681 agagatggta aagggataaa aactgtgctt tatggaaatg agattaatgc tataaaaaat
     4741 gttggaaaga cattgccaga gatgtttaga ctaaagggtg atttaaaaga tgttgaggtt
     4801 acaatattgc ctaaagatag tattttaaaa gagtttcaaa atgtttttat tgccatgatt
     4861 ttaattactg ctatgtttat gggatgcacc tttaatgcaa tgaatattat atctgaaaaa
     4921 gaagatggaa tatcatttat aaatgaagtt ttgccacaga gtagaagtca gtatattata
     4981 caaaaagtat ttttgggtct tttatgtggg tgtttatctt caatgataac tgtatttata
     5041 tgtttggata tttcaatatt agatatgata atgatgttgt tactcgttgt attttcagca
     5101 tttatttcat cacttatagg tttatttatt ggaaagttgt cagaaggtct tatggtagga
     5161 attgtttata ttaaaatagt aatgatttta tttataggta ctcctttgtt aatatatctt
     5221 ctaggggtta ataccagcgg tatagggaat ttatgctata taatgccatc agttgctaca
     5281 tttaatggta ttatggagat tattaatggt aatgtcatta ttataaaaga tattgtaatt
     5341 ttggctgtac attgtgtagt atggtttatg atatatatta ttttagataa aaaaagaaag
     5401 ttatctttta gtagataact cagtataatt ataattggga caatttaata ggtgatttaa
     5461 tatactttgt atagtgtaat ttatgtagtg caatttgtat actttaattt atgtagtgta
     5521 atagttagtt ttattttata attattcaat ataatttagt aagaagttca gtacaattag
     5581 agctacttag tggaaaactt aataggtact taaactatat aaatacttta ttttcctcca
     5641 tttgtactat aaatttaatt tatgatataa atggaggtat tattatatga tttgataaat
     5701 ttc
//
LOCUS       DAELJO010000221         5699 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELJO010000221 DAELJO010000000
VERSION     DAELJO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.223"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            168..797
                     /locus_tag="KPB88_003418"
     CDS             168..797
                     /locus_tag="KPB88_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055656.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(911..2425)
                     /gene="glpK"
                     /locus_tag="KPB88_003419"
     CDS             complement(911..2425)
                     /gene="glpK"
                     /locus_tag="KPB88_003419"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0055657.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVDDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2669..3211
                     /locus_tag="KPB88_003420"
     CDS             2669..3211
                     /locus_tag="KPB88_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055658.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3225..>5699
                     /locus_tag="KPB88_003421"
     CDS             3225..>5699
                     /locus_tag="KPB88_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0055659.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRTKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYK"
BASE COUNT         2117 a          871 c          785 g         1926 t
ORIGIN      
        1 agttttaagt ataataaaat catgtatact aaatgcatgt taaaatatta ttttaaaaca
       61 tgcacaatat ttgtacatat tgtagacata taaacctata aattatacaa tagtattgtt
      121 aagattatat atttttttaa cataaatttt gtattggggg aaaaacaatg gataaaaact
      181 atacgatgcc aatataccaa aaaatagcgt tggatattgc aaataaaata tacacgggtg
      241 aaatacaaga ggattcagta ctatttggac gctctgtttt ggctggtaaa tacaatgtat
      301 ctccagaaac gataagaaga gctgtcaaaa ttttagagga cattggcgtt gttaaaagca
      361 taaagggtaa aggtgttatt gtactatctc cagacaaagc ttcttctttt ataaaaaaat
      421 atagagatat tacaaatata tcatcatata aatcaacact ttataattta atagatacta
      481 aatctaattt agaaaatgaa atattagata ctataaataa aattttagac tattctaaca
      541 gacttgaaat aatcaatcct ttagtgcccg ttcagttcac cataaattct aattgcaaat
      601 atattggtca aacagctgca caaactaaat tttggcaaaa tacaggtgct acaatagttg
      661 caattaagag aggtgaagaa cttataatat ctcctggacc atatattgaa tttttagaag
      721 gagacattct cttagtagtt ggtgaccaac acatttacaa ttctatacca atgtttttat
      781 atgaaaacga aaaataaatt atataattta gtatgattat ataaaaatgc aaaattaaca
      841 acaaaaaaag agcttcatat tttacataaa gctcttttta aattcaaata aatttaactt
      901 tgattaattt ttaaatatcc aattcatctt ctttttccca gcttaaagct ctcgatacag
      961 cttttttcca tcctttatat aatttacatc tcttctcttc actcatattt ggattaaact
     1021 ctttttctac tgaccacttt gaagtaattt catttcttcc aatatagaat ccaacagaca
     1081 accctgctaa ataagcagct cctaaagctg tagtttctac tattttgggt ctatctatat
     1141 ctacatttaa tatatctgat tgaaattgca ttaaaaaatt attattactt gctccaccat
     1201 ccacttttaa tgattttagt tttaattttg aatcgttttg cattgcttct aaaacatcct
     1261 ttgtctgata agcaattgat tccaatgtgg ctcttactat atgttctttt tttgctcctc
     1321 ttgtaagtcc taaaattgaa cccctagcat acatatccca atatggagct ccaagacctg
     1381 taaatgctgg cactacatat acaccattag tatcatctac cctatttgca tacatttcac
     1441 tatcttttgc agattcaatt atctttaatt catctctaag ccattgaaca gatgctcctc
     1501 caataaatat acttccttct aatgcatatg ttacttttcc atcaactccc caagcaattg
     1561 ttgtaagtaa cccatgctta gatttaacta tgttactgcc tgtattcata agtaagaaac
     1621 aacccgttcc ataagtattt tttgcagtac cttcttcaac acaattttga ccaaataatg
     1681 ctgcttgttg gtctccagca catcccgcaa taggtatttt agctcctgat aacatgcgct
     1741 catctgtatg accataaaca tagctagaag gttttactaa tggcaacatg cttattggta
     1801 tgtccaattc tcgtaatata tcctcatccc attctaaatc atttatattg tacatcattg
     1861 ttctagatgc atttgagtaa tctgttacat gtacttttcc acgagttaaa ttccatatta
     1921 accatgtatc tatagttcca aataataact ctccttgttc tgctttttcc ctagctcctt
     1981 ctacattatc taatatccat tttacttttg ttgctgaaaa gtatgcatct attaaaagac
     2041 ctgttttttc ttttattgat tcttccaatc ctttattttt aagttcatca catatttctg
     2101 acgttctcct acactgccat actattgcgt tataaatagg tttaccagta tacctactcc
     2161 aaacaaccgt agtctctctt tgattagtta tccctattga agcaatttct tctggtctta
     2221 tacctgctgt ttctataact tctctcatta ccccactttg tgacccccat atttccatag
     2281 ggtcatgctc tacccatcca gcttttggat aaatttgatt gaactctttt tgtgcaattt
     2341 tcactatttc gcctttttta ttaaatagta tagctcttga actggttgtt ccttggtcta
     2401 atgccattac atacttcttt tccatcacca tattctcctt atcatataat atttaattta
     2461 ttaaaaagaa aaatttaata tcacatattt caataaataa tcatacttaa gtacaaattt
     2521 aatataatag atatttagta agaagacact tattttctat catatctata taataatatt
     2581 atacattata gcttacatat tgattaataa attttaaagg agtataataa acagtgttca
     2641 taataatttt aataaaagag gtggtaatat gccaaagatt ttagaaaatg taaaagaaga
     2701 tatattaaaa gtgtctcggg atatgatttt agaagaagat tactcgaata ttagcataag
     2761 gaaaatagct caaaggtgtg gtataagtgc aggtacagtg tataattact ttaattcaaa
     2821 acaagaaata atagaatata tcacaaaatc tgaatgggat ttattgataa ggcgaattga
     2881 atactccaat aaaaacactg aagattacat aaaaaagcta aacattattt ttactgaaat
     2941 aagaaatttt ataaacaaag ttcataatat ccaatacaat gactttttaa acacttttga
     3001 aacagagaga ttttttgaaa tgaaaaaaca caaagacgac tttcatgaac agctttcaaa
     3061 taaagtatat gaagctttag aaaaagaaag tttttttaat aatgacaaat tagtatgcaa
     3121 tatcataata aaaatgtttt tttcatattc aacatatctt catatagaat ttgaagattt
     3181 aaaaccctat attgaaaaat taatacatta aggtggtgtt taccttgaat cataatacta
     3241 gtattaagaa caaggacagt aatccaaaaa tatttttagt acttacactt tatctattag
     3301 gtatatttat gggagctatt gatactggaa tagtatcacc agcaagaacg attatacaaa
     3361 atagccttgg agtaaatgaa aaaacgggaa tttggatgat taccatatac acattaacat
     3421 acgcttctgt aattcctatc tctggaaaat tagcagataa aataggaaga aaatatgtgt
     3481 atctagttag tatatttcta tttggtagtg gttcattaat atgtggatta tcatcactat
     3541 tttccaattt ctatatttta ttaattggtc gtgttattca agcaattggt ggtggtggaa
     3601 tcatgccaat tgctacagca gaatttggaa ctaccttccc agaaaacaaa agaggcatgg
     3661 ctttaggttt agttggggca acatatggta ttgccaatat tttaggttca agcattggta
     3721 gcacaattct aagtatcttt ggaactcaaa attggaagtg gctatttttt gtaaatcttc
     3781 caatttgttt aatcatactt attggtggag ttttctgtat taaaaacaat aagtctgaat
     3841 ctaaagaaaa aatagataag cttggtacat tgatgttgat atgtataatc gtatcactat
     3901 tatatggtct tatgaatata gactttttta attttaaaaa ttctatacaa gatatatcag
     3961 tttatccata cttattgaca tttattgtac ttatacctat tttcatattt attgaaaata
     4021 gaactaaaga ccctatttta agctttgaat attttttaaa tccgagaata ttaatagtac
     4081 ttattttatc tttaattgtt ggtattggaa tgatgggtat ggtattcgtt ccacagtatg
     4141 ctgaaaatgc attaaagata aatgctggtt ctgggggata ttttgtagct atattgggat
     4201 tatttgcagg tattgctgct cctttaagcg gaagacttat tgacaaattt ggggcaaaaa
     4261 aaatactatt actaggattt tctgtatcta tgataggctc tctttaccta gttttaattg
     4321 ctctaaaaac caatactgta tttagtgtat gtgtaagttt aatatttatg ggattaggaa
     4381 tgggatttac tatgggaact cctctaaact atatgatgtt atcaaataca agattagaag
     4441 aatctaattc agcacttgca acattatcct tgatacgctc aattggtact tcaatttctc
     4501 cagctataat gattggattt atagcacatg caggattatc tgtgcaagat aatattatgg
     4561 acttagttgg aaagccctca actcctaaaa tagttcaact tgaagaactt gaaaacatga
     4621 tagatgactt aaaatcagac cctgaaatgg caaaaaattt aaaaaatgtc tctatcccaa
     4681 atatgaatga tacttcaaat ataaatatgg acatgacagg tggcaaactt ccaaaatcct
     4741 tacttaataa agttcagtca gcagatacaa ccaacattac caatataaca aaagaaatat
     4801 caacaacaat gtttgatgaa aaagttccat ctataattac taaaatacag aaaaatgtac
     4861 aaaaaggaat agatgggaca caaagtggaa taaacggtat tgaagatggt gaaaataaac
     4921 taaatagtgg tattaaagga gttcaaactg gtattgacaa tatggtaaaa gctagagctg
     4981 gtattaatca aggaatagat ggtgtaaaaa aaggtattgc tggtgttgac aaaggtataa
     5041 aaggtatgga acaaggtctt gaaaaacaag atgaggccat caaggaactt acaactgctt
     5101 ataataaaat acctactaca gaaaaaactc ctactgataa cgaaaataac tcaaatgaac
     5161 aaaaccagag taataccagt aattctgaat cagatgacac taagaatgat tctacagaaa
     5221 ataagaataa caattccagt gagaataaac caaatgtaaa tgaacaaaaa gagagtttaa
     5281 atgctcaaat acagcaacta aaaaagacaa gagatgactt aaatacaaag ttacaaaaaa
     5341 ataaaactca aaagaacgaa ttaagtaaaa aactaagatc aatggaatct caaaagaaag
     5401 agctacaaag caagttaaat aattctataa gccaaaagaa agacatggaa aaaagccttg
     5461 atacaatgca acagcaaaaa aaatcactcc aagcagtttt agcaaagaca caagaggtaa
     5521 aaactgaaat accaaaggct tttgataaat caaaattgga ttatataaat tccatagaaa
     5581 ataatagaac aaaaatagaa aacacactac aatctactct aaactcagga tttaaacaga
     5641 tgtatataac agtattctgt gtaaatctac tagcatttat aatattattg ttctataaa
//
LOCUS       DAELJO010000222         5674 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELJO010000222 DAELJO010000000
VERSION     DAELJO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.224"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            123..1700
                     /locus_tag="KPB88_003422"
     CDS             123..1700
                     /locus_tag="KPB88_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0055660.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            1848..2600
                     /locus_tag="KPB88_003423"
     CDS             1848..2600
                     /locus_tag="KPB88_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0055661.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            2739..4079
                     /locus_tag="KPB88_003424"
     CDS             2739..4079
                     /locus_tag="KPB88_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0055662.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPILYKENEK"
     gene            complement(4191..5387)
                     /locus_tag="KPB88_003425"
     CDS             complement(4191..5387)
                     /locus_tag="KPB88_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0055663.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         2075 a          720 c          911 g         1968 t
ORIGIN      
        1 tgaaaaaatt actatttatg taaaaatatt ttaaaacaaa ttataaagtt tcaaaaacaa
       61 aataaaaata tataattatg acatagaaaa cgttttaaaa atttaaaaat ggagggatag
      121 aaatgaaaca aaaaatgaaa gggttctttt tacagttagg aaaatcattt ttattgccaa
      181 tagcattaat atctgcagca ggtatatttt taggaataag ttcagcattt tcaaatccta
      241 atatagttag tgatatgcct tttttagcaa ataattttat tcaaggaata ttgggtttta
      301 caaaagctat aacaggagta ctttttggaa acttagcaat attctttgct atatcaatat
      361 ctgttggtct tgctaaagaa gaaaaagcag tttctgcatt ttctggtttt gttggttatt
      421 tagtattaca cactagtgta aattatatgt tagggtgtag ggggattctt gctgaacctg
      481 acatgatgag agaaatgggt caagctgtaa ctttaggcat acaaacatta gaattaggag
      541 tatttggtgg aattgtagtt ggtataataa cagcatcatt acataataaa tactataata
      601 taaaactgcc agattattta ggattctttg gtggaactag gtttgttcca ataataacaa
      661 cagtagtatt ctctattttg gggataataa taccatatat atggccatat gtaaatacaa
      721 ttattcagag tgtaggtatt ggtatagcta gtttaggata tttaggaaca tttgtatttg
      781 gatttcttga aagactttta ataccatttg gattacatca tatattaaat gctatgttta
      841 gatttactga agtaggaggt tctttagttg tgggagggca agaagtattt ggagcattga
      901 acatattctt agctcaacta agtgaccctg aaacaatcag tttttcaaca gaagcaacta
      961 gatttttggc tcaagggaaa atacctataa tggtatttgg tcttcctgct gctgcatatg
     1021 ccatgtataa aactgcaaag cctgaaaata ggtcgaaggt taaaggtata ttactagctg
     1081 gtgctttagc aacatttgta acaggaatta cggaaccatt ggaattttca ttcttatttg
     1141 tatcaccaat attatttgtt atacatgcat ttttatcagg tatatcattt atgatgatgc
     1201 atatatttgg agtagcaata ggaaatactc aaggtggcat aatagattta gtagtatttg
     1261 gagttatgca accaaataca aagtggattt attctataat acttggttta gtgtatgcaa
     1321 tagtttatta taatgtattt aagattttga tacttagatt taacttacaa actccaggac
     1381 gagaagaaat aacagatata aattttgatt taaaaccttt atcaaataaa gtagaaatag
     1441 ctcaaagtgg agaaactact aagagagcat tatctataat taaatattta ggtggtaagg
     1501 acaatataga agatataaca aactgtataa ctcgcttaag agtagtagtt aaagatatga
     1561 gtaaagtaga tgaaaatgct tttaaaaaat atacaggtgc aatgggagtt ataaaagtat
     1621 ctgataaaga tatccaaata atttatgggc catctgtatc tcaagtaaga gacgaagttt
     1681 cttttgcaat gtctatttaa agtaaatatt aaaaggcaat actaatttat aatttgtatt
     1741 gtcttttttg tagtataata aacataagat aaatgctgta tagtgtatat ttatataata
     1801 aatatgagaa agttgtttaa ctacaaatca ttattatgga ggtataaatg aacctaatag
     1861 acaaattaga tataaatcaa aaaaaattat ctacaaatga aatatctctt ttaaactatt
     1921 ttctaaaaaa ttctgaaaaa ataaatcaaa aaaagattaa agatatagca aatgaaactt
     1981 ttaattcgac tgcatacata gtaaggtttt gtcagaagct cggcttttct ggatatagtg
     2041 aatttaaaaa ctttttagtt ttttcatatt caaatagaaa tgaaagaaat aaggagttta
     2101 ctaaaagtca aagtgatatt tttactgatg ttatggatac acaaaattta ataaatgtag
     2161 agaatataga tatgtgttta aatgaaatac ataattctaa gcatatatat ttttttggtg
     2221 ttgggtcttc tagattagta tgtactgaaa tgtctcaacg tttttcagct ataggtgtaa
     2281 atgtaaaata ctatgatgat tcaactctca tgtatttggc tgcatcaaat attactaaag
     2341 atgaccttgt aattgctgta tctatgtctg gagagactgc tcaagttatt aaggcatgta
     2401 atatagcaaa aacaaatcaa gttaaagtaa tttctctgac aaatataagt gtcaattcat
     2461 tatcaaatat agctgatata aaattatttg tatcttctcc acaatataat tttaatagtg
     2521 ttaactatac atctagagtg ccagcacttt ctcttatgga atatatgttt cataaataca
     2581 ttgaaaaata caatagatga aaaaaattac aattttgaaa aaaatactaa aattgaacta
     2641 tttttaatga ttacctaaaa gtattcatgt agaaaacttt atatattata atcatcttac
     2701 aagttagtta ataaaaaaca ggggtgatta atatgacaat gaaaaaaaga tacaatatag
     2761 ctatagttgg aggaggcagt acttggactc caggactttt aaagtcttta tgtaaaatga
     2821 gtgatagatt tcctctaaat aaagtaacta tgttagatgt agtagaagaa cgtcaaagat
     2881 taatagggga atttggaaaa atattattta aagaagaata tcctaaagca acacttgaat
     2941 atactacaaa cccagatgaa gcatttatag atgtagattt tgtatttgtt caaatgagaa
     3001 ctggtggtct taagatgcga gaattagatg aaaagattcc tctacaattt ggactggttg
     3061 gtcaagaaac ttgtggagct ggtggatttg cttatgggtt aaggtctata ggtgatatga
     3121 tagaaatggt taagactgta agaaaatact ctcctaaagc atggatatta aattatacaa
     3181 atcctgctgc tattgttgca gaagcattaa aaagagtatt tccagatgat aaaagattac
     3241 ttaatatgtg tgaccaacca gttaacttgt taaggtctta tggaagattg ttagacatgg
     3301 attcaagaac atttgagcct gtatactttg gattaaatca ctttggatgg tttacacatt
     3361 tgtatgataa aaatggtgaa gatttggtac ctaagataaa agatttagta gcaaactctg
     3421 gattccaacc tgttgatgca gaacaaagag ataaatcttg gttagacact tatggaatgg
     3481 ttaaagatat gttggaagat gtaccagatt acttacctaa tacatatgta cagtattatc
     3541 tatatcctga taaaaaagta gctaaatcaa atataaattg tactagagca agagaagtta
     3601 tgaatggaag agaaaagcgt gtatttgaag aatgtaaatc tgttatagaa aaaggaacat
     3661 ctataggttc taatttagtg cataatgatg ctcatgggga atttatagtt gaagttgcag
     3721 aagcaatagc tcataacaaa caccaaatag ccatagttat aacagaaaat aatggggcta
     3781 ttaacaactt acctgatgat gcaatggtag aagtagctgc gattcttaca aagaatggac
     3841 cgagaccttt acatgtaggt aatataccaa cattttataa gggactgtta gaaggtcaac
     3901 tagcttatga aaagttagca gttgatgcat actttgaaca atcttatgaa aaagctattt
     3961 tatctttaac attaaataga actataatct ctccaactaa ggcaagacaa gttttagatg
     4021 ctttaattaa agtgaataaa gattactggc caatactata taaagaaaat gaaaaatagt
     4081 caggtctaag tcttaattaa tatattaatt aagaaatgtc cccttttcaa ataatatctg
     4141 aaaaggggac attttatatc attaaattta tataaattaa gtttaaaaaa ttatttttgt
     4201 aaagctacat attgctctaa accttctttt aataatgtag cagctctatg aaggtctttt
     4261 tcatttaaga tgtaagctaa tcttatttca tcacgtccta aacctggagt agcatagaat
     4321 ccttcagcag ggcaaaccat aactgtttca ttatctttgt taaattcttt taacatccaa
     4381 attgtgaaat cttctgcatt ttcaactggt aatttagcaa ctatatagaa agcaccagtt
     4441 ggttttttac atataacacc ttcaaccttc attaattcat tgtataatac atctcttctt
     4501 tttttgtact cttcattaac ttctttgaag taagaaacag gagttttgta taactcaaca
     4561 gctcctattt gctctaatgt aggaacacaa agtcttcctt gacataattt aagtatttga
     4621 gcgataagac ctttattttt gcaagcgata gaaccaattc tagcaccaca agcactgtat
     4681 ctcttagaaa cactgtctat tattataact ctatcctgta caccctctaa gttaccacag
     4741 cttgtatact caagcccatc ataaacaaat tctctataaa cttcgtctgc tattatccat
     4801 aaatcttttt ctttagctat ttcagctagg atattaagtt cttcttttgt ataaattgca
     4861 ccagttggat ttcctgggtt agaaagaatt atagccttag ttttatcatc tactttagac
     4921 aatatttctt ctttagatgg aaggtggaaa ccgttttctg cctttgttgt aacagcatta
     4981 acttctacgt ttacagattg accaaatcca ttgtagtttg tatagaatgg ttctggaact
     5041 aatagattat ctcctgggtc acaaactgcc atcatagtga aaagtaaagc ttcactacca
     5101 ccattagtta ctaataattc atctttttca aaattcatat cgtaagtttt atagtagttt
     5161 tgaagtgctt ctaataattc tggaatacct tctgatgtag catattctaa gacttcgcta
     5221 tcaaagtttt taacagcatc aaaaaatcct ttaggagttt ttatgtctgg ttgtcctatg
     5281 tttaagtgat atactttgat tcctttatct tttgctgctt gtgcaaatgg aacaagtttt
     5341 ctgattggag aggcttgcat tgcactaact ctgtttgaat aattcatttt aataccccct
     5401 atttaattta aatacgtata ttgtattatg taaattgtat gtttgaatac aagacatctt
     5461 tacaagataa taataacata cttatgggta aaaatcatca aaaaaaatta atatagcttt
     5521 aaaataaact tttaacaaat tatagtaaaa tacccatgtt tatgccacta aattatgtat
     5581 tcataaatta atttatagta agataatatt ctgatatact gatagtgatt ttttagttat
     5641 aatctttcag ctatagacaa aatatataat aata
//
LOCUS       DAELJO010000223         5659 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELJO010000223 DAELJO010000000
VERSION     DAELJO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.225"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            180..1448
                     /locus_tag="KPB88_003426"
     CDS             180..1448
                     /locus_tag="KPB88_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0055664.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1643..2431
                     /locus_tag="KPB88_003427"
     CDS             1643..2431
                     /locus_tag="KPB88_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0055665.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRILETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2431..2880
                     /locus_tag="KPB88_003428"
     CDS             2431..2880
                     /locus_tag="KPB88_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0055666.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            2884..5148
                     /locus_tag="KPB88_003429"
     CDS             2884..5148
                     /locus_tag="KPB88_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0055667.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5288..5599
                     /locus_tag="KPB88_003430"
     CDS             5288..5599
                     /locus_tag="KPB88_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055668.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2155 a          609 c         1089 g         1806 t
ORIGIN      
        1 ccttttaata actagcaaaa aatagcaaaa ttatataagc gttatttaat tgtcaaaaat
       61 gaaagctaaa ttgcatgtcc taaaaaaaat gattgataac atttatttgt gtgctataat
      121 tatatagtaa aacaataagt atattgcata atgtatgcaa atcgggagga gagacggaca
      181 tggaagcatc tatacacaca attgccagca ataatttatt gataattttt gctattgtaa
      241 gtataactgg aataatttgt agtaagctta gtgaaataat aaaggttcca gatgtagtgt
      301 tatatttact tgtaggtatt ttaataggac catcattttt aaaatttata gatattcgtg
      361 gatttcaaat agaaaatcaa ctaatactta catttggttc tgcatttatt ttatatttag
      421 gtggaaaaga aataagttta aaagtattga gaaatgtgaa gatatctgta ttcttattag
      481 ctactttagg agttgtgata tctgctttta taatgcaaca agtaatagga tttacttttg
      541 ggataagtgc aatgactgct ttactagcag gaactataat agcatcaaca gacccagcca
      601 ctcttgtacc tatatttaat caggtaaaga taaaggatag ggtaaagcaa actgtaataa
      661 gtgaatcagc ttttaatgat gcaacaggag ctatactaac atcagcagtg ttggctgtaa
      721 ttttgtcaaa taaattttca ttaggtgaaa atatttatga actggggaca atggttattg
      781 tcggagtttt agttggttta attacaggaa tactgctttt gaaattagtt aatgataaac
      841 cttatggaat atttaaagat tttgcaccaa taatatctgt tatttcagtt gtagttgcat
      901 atgaattatc tacaaaatta ggtggaagtg gatatatgtc ttgctttatt gtaggtataa
      961 taactggtaa caagaaaaac tttaaaattt ggttaagtca aaaatcttat gatgcagatt
     1021 tttatgttgc tgaaacttta ggtactattt gtcgtatggc aatatttata atattgggta
     1081 gccaagttaa tttggctcag ttaagtaaat attttatgcc atctgtaatt acagtattag
     1141 tattaatgtt tatagcaaga ccactttgcg ttttagtttg tacacttgta gacagggaag
     1201 caaaatggag taaaagtgaa atattattta tgatgtgggt aagggaaaca ggcgtaatac
     1261 cagcagcact ttgtggtatt atttcagcaa tgaaagttcc tggatacgaa gtgatttcat
     1321 ctgtagtatt catgactata ctaattacat tagtaataca aggaagtact acaaaattag
     1381 tagctaaaaa acttggatta ttagaagaag aagtgataaa tataagtgaa aaagtatcaa
     1441 ctttttaaag ttttgcttta aaatagtacc tgttaatggc tattttaata ttgtctaatg
     1501 atgattattg aaatagtcat ttttttatgt ataagtaatt tacagaattt acatattaga
     1561 catatttatt atcaaaattt gttgaagcta tgatataatt agttgtattt aaaataagaa
     1621 tagcaaataa gggaggaaat ttatgtttac ggttatggat attgtacagc cagatacagt
     1681 agaagaagcg tactcaattt taaataaaag aaaaactaac caagtaattg gtggaagtgc
     1741 ttttttaaga atggggaaaa aaagaatagg aacaggaata gaattatcta aattaaattt
     1801 agattatata aaagagtatg aagattacgt tgagattggt tctatgacta cttttagaat
     1861 attagaaaca agttccataa taaaaaataa ttttggaaga ataatagaag attcagttaa
     1921 aaatataata ggagttcagt ttagaaatgt agtaacagtt ggtgcaacag tattttctaa
     1981 atatggtttt tctgacttga tagtagcatt actttcatta gatacagaag tagaattata
     2041 taatattggg agaattagtc ttgaagagtt tctaaataga gattatgaga aagatttact
     2101 tattaagata tacataaaga agacaaataa gaatgcttct tataaatcac ttagaaatgc
     2161 aaagagtgat tatccaattc taaatgtatc tgtatcaaaa catatggaac aatttaagtt
     2221 atgtgtagga gcaagaccgc agaaagctac gattgcaaaa caagctagtg aatttctatc
     2281 taacaatgaa atcaatgaaa ttaatataga taaagcagta gaaatagcat cagaagaact
     2341 aacttttggt tctaacatga gagcatctag ggagtataga aaagctatga gtaaagtact
     2401 tttaaaaaga gctataatgg aggttattta atgttagtaa agattaatat aaatgggaag
     2461 aaaaaaagtg ttgatataga accagaagaa tatctggttg atactttaag aaaagttggt
     2521 aatttaagtg tgaaaagagg ttgtgacaca ggctgttgtg gtttatgtac agttttaatt
     2581 gacaagaaac ctacattatc ctgtgcaaca ctgacattga gagctttaga taaagaaata
     2641 actacaattg aaggtttaga aaatgaagtt aaagaattta gtgaagtttt ggtaaaagaa
     2701 ggagctgagc aatgtggttt ctgttctcct ggctttatac ttactgtact tgcaatgaag
     2761 gatgagcttg aaaatcctac agatgatgaa ataaagcatt atcttacagg aaatctatgt
     2821 agatgtacag ggtatatggg acagttaaga gctatcaaaa cttatctgga ggtaaaatag
     2881 aacatgaata tagttggaaa aggaatcaag aaaatagatg gaatgagtat cacaacagga
     2941 aagcctgtat atacagatga tttatcagca aaagatgctt tggtggtaaa gatacttaga
     3001 agtcctcacg cttatgcaaa aataaaagat atagatacat caaaggcaga aatggttgat
     3061 ggtgttgaat gtgttttaac atataaagat gtaccaaaat ctcgatttac acttgctggt
     3121 caaacatatc cagagccatc tccatatgat agattaattc ttgaagatgt tgtaagatat
     3181 gtaggtgacg aagtagctat agtggcagct atagatgaaa aaactgcaat taaagcaatg
     3241 aatcttatca aagtaaagta tgaagtactt acacctgttt tggattttga agaggcaata
     3301 ggaaataaaa ctatggtaca tgatgaagat agacattgta actttgatat aggtatgcaa
     3361 agagaaaaaa atatagtatc ccatcatgat tatacaaaag gtgatgtaga aaaagtatta
     3421 agtaattgtg atgtagtgat tgaagaaact tattatactc aagctcaagc acatgcaatg
     3481 atggagacgt atagggcata caattattta gaccatatgg gaagattggt tgtagtaagt
     3541 tcaactcaaa taccatttca tgtaaaaaga catttatctc gtgcactaga aataccagct
     3601 agcaaaatta gggttataaa acctagaatt ggtggcggat ttggtggtaa acaaactgca
     3661 tgtgttgaga ttttttctgc aattgttact ttaaaaacag ggaaaccagc taaaataatt
     3721 tatgatagaa aagaaacttt taattgttca acaagtagac atgcaatgag attaaaagtt
     3781 agactaggtg ctacaaatga tggcataata aaggcaatag atataaaagc actttctgat
     3841 acaggagcat atggagagca tgcttctaca actattggtt tggttggaga aaaaacttta
     3901 ccaatgtata ataaagttga agcaacaaga tttattgcag atgtggtata tactaataag
     3961 atgccagcag gagcacttag agggtatgga gcatcacaag gatgttttgc tgttgaatca
     4021 actataaata tgcttgctaa taaattaaaa atggacccaa cagatttaag gttaaaaaat
     4081 gtggtaacag aaggtgaaac tacatttgca tatggtaaaa ctttaaatag tgtcgattta
     4141 ataaaatgta taaataaagg caaagaactt attggatgga atgaaaaata tccttgtaaa
     4201 catcttgata atggaaaaat tagaagtgtg ggtatggcaa ttactatgca aggttctggt
     4261 atacaaggtc ttgataagtc ttcagctgaa ataaaattga atgactgtgg agattatact
     4321 cttatgatag gttctactga tatgggtaca ggaagtgata cgattttagc tcaaatggct
     4381 gctgaggttc ttgatacaag cattgaaaat ataacagtta tatcagctga tactgactta
     4441 gttccatatg acccaggttc atatgcttca agtactactt atgtaacagg tatggcagta
     4501 gttaaggcag ctcaagactt aagagaaaaa ataatttctg ctggagcaac taaactaggt
     4561 gttgagaagg atgaagtaga atttgatggt ataaaattaa ctagtaaaaa aggtagcact
     4621 ttattattcg atatagcagt tgattctaca attggtccag atactaatca attaataggt
     4681 cttgcttctc atggtagccc agtatctcct cctcctttta tagcaggttt tgcagaaact
     4741 gaaattgaca cagaaactgg aaaagttgat ttagtaaatt atgtagctgt agtagattgt
     4801 ggaacggtta taaataaaaa tctagctaag atacaagttg aaggtggaat tgttcaaggg
     4861 attggaatgg cattatttga agaagttaga tacacagaaa aaggaagaat ggacacaaat
     4921 acatttatgc aatacaagat tccagctaga tgtgatattg gaaatgttaa agtagatttt
     4981 gttgaaactt atgaaccaac tggaccattt ggtgctaaat ctgttggaga agttgttata
     5041 aatacacctt taccagcaat acaagaagct gtgtttaatg gaattggtgt aagaataaat
     5101 acactcccta ttacacctga aaaagttttt atggaaatga ataaataatt atttatgaaa
     5161 atttagacta gttttacatg ttaaacaacc agttatgtca tacctgttga aagatattta
     5221 ataagtagtt attataatat tatgaatttt acttataaaa tacaaaatta aataagggga
     5281 tgatgttttg gctggtaatc taaataatat gagagcagta aataatttta gaggagataa
     5341 gaacatttta gaatgtttag tcagctttga gggtcgttca ataagtcaga gaaaagtaag
     5401 ggtatttttt aaagaaaaac aaaatcaaat agaaattgat tttgcagaag aggaaatttc
     5461 taaattggtt gaaaatgttg ttttaaatac atcatatcaa gaaatgttat atgatgaaat
     5521 agagaaacaa ctggaaattg attgtatagg tacttggatg atattatcta aattaaaaga
     5581 tggtagtaga gttcactgat tttaaaataa attattagag cctaatttta aggctctttt
     5641 ttgctgtttt taattaaca
//
LOCUS       DAELJO010000224         5646 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELJO010000224 DAELJO010000000
VERSION     DAELJO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.226"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            161..661
                     /locus_tag="KPB88_003431"
     CDS             161..661
                     /locus_tag="KPB88_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG0055669.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            718..1704
                     /locus_tag="KPB88_003432"
     CDS             718..1704
                     /locus_tag="KPB88_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0055670.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1754..2476
                     /locus_tag="KPB88_003433"
     CDS             1754..2476
                     /locus_tag="KPB88_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG0055671.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2505..4439
                     /locus_tag="KPB88_003434"
     CDS             2505..4439
                     /locus_tag="KPB88_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0055672.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4432..>5646
                     /locus_tag="KPB88_003435"
     CDS             4432..>5646
                     /locus_tag="KPB88_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG0055673.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDD"
BASE COUNT         2359 a          428 c          971 g         1888 t
ORIGIN      
        1 atttgtatgt attatattgt ataatgtaaa atgaattgga aaaatagttc gataaaataa
       61 tttgttttta taaaattact gtttaagttt atagattttg tataaaataa agttatgtta
      121 ttaatttttt tagaaataaa acttttacta ggagacagat atgagaaaaa atagtattaa
      181 tatagcacta actgcagcaa ttacagcaat ctatgcagtt ttaacattat cattgggatt
      241 tataagttat ggaccaatac aatttagggt agcagagatt atgatgcttt tggcattttt
      301 agataaagga tatatagtag gtcttacatt aggttgtttt ttagcaaatg taataggacc
      361 atatggtgta cctgatataa tttttggaac attagccaca tttataagtg caagtatgat
      421 ttatattact agaaagttag gtaaacagaa taaaagtact ttagttatag cttctttatg
      481 gccaactgtt ataaattcga ttgtggttgg tctaatgtta aatgtattct ttgggcttcc
      541 attaattcta tctatgatac aagtaggttt tggagaattt gtagtagtga caattgtagg
      601 agtaccattt ttctgctttt taatgaaaaa atataaaaat gtcattaata ttaaatttta
      661 aatagtgtat agtaatttaa ttaattgaga gtattattag tgaaaagagg tgtttatttg
      721 tcgaaggtaa aaagattgac tgatgtttgg atatatatta atgataagca aaagtttaca
      781 ataaaagagt tgtcagagga atttaatctt tcagctaaaa caatacaacg atatttaaaa
      841 gaattaaata aaatgggact tccaattcaa gcagaacaag gtagaaatgg aggttatagg
      901 gtgctaaata atagctatat acctcctgtt atatttacag aaaaagaagt tatgattata
      961 ctatttgcac ttaagtcact tcagatttat aattataagt taatggaaat agaaattaat
     1021 tctataatga gaaaagtaag ttatgaacgt aaaagcagta taaaagtagg tatagaaaat
     1081 attaaaagat acattggctt tgtagctaat gatactaatg atggagattt taagagtagt
     1141 aatgtaactg agttttttag agcttcttca gaatctctaa ttttaaatat taagtataag
     1201 tgtggtaatc agattttgga aaaaaacgta tgccctatag gtatatattt aaataaggga
     1261 gcatggtttt caccagcata tgattgtaaa agtgataaaa taatattagt tcatatagat
     1321 aatataattg atataaaaag aacaggagaa agtaaaaaaa tacatataaa cttggaagaa
     1381 tggttacatg aagtatataa caatttatat ggaaatagtt caactactaa aaaatttggt
     1441 gaaattattt atctaaatgt attattaact aaagaaggtg tttctaaaat aaaggacagt
     1501 tcttggaatt tagatatgga gctaaatgaa gatggtagtg gaataattag tacattcata
     1561 aaggttgaag atatagattg ttatgcttcg attttatttt ttattggaaa taatgctaag
     1621 gttatagagc caaaattttt gaataagcta ctttatgata aggccaatga gttaagatat
     1681 ttttatgaag aaaatatggt ataatattta gaaatgttga aattaaataa ttattaagga
     1741 gatatacgtt ataatggaaa attttaataa gaatgctaaa ataattggta ttggagctga
     1801 aggtattaat ataataaatg aagtagaaga aaaaataaaa gcaaatatgg atatagaaaa
     1861 aataaacata aatcaagaga ttgaaaaaga atatgttaga agtcttttag atggtgtaga
     1921 tatattattt ttgatatatt catctgaaga taaacatatt agagatatta taaaagctgt
     1981 ttcatatatg tcaaacgaaa gaagagttct atctatagga atggattgtt cagagaaaga
     2041 aaataaagaa gatttagagc ttgggagaga gtttaaaatc aataatgata gtatatttaa
     2101 atttgttgat ttaatgaata tcatggtaga gtctatttct gattcttgca tgataaatat
     2161 agatattact gatttaaaag aagctattgt aggagataaa ggaattaagt attcttttga
     2221 agagtttgaa gatacaaagt catatagcga gatagctgac attttatttg acaggatgga
     2281 atatattggt gaggaattta taagtaagaa aggaattgta tttgttgaag gaagtccaga
     2341 attttctata atggaattga atgatttaat aagtaatatt cagagcaagg tagaagaaag
     2401 ctatgaagta atattttctt tatatataaa ggaaaattta aatggaaata ttagagtagg
     2461 cttattgtat aattagtata ttttagatat taaggagagt gtatatgtct gaaaatataa
     2521 gaggtacaga gtattggtta atggaagaat ttttttatca ggaaacagac acaccacaga
     2581 tttttagaga agaaagcata attgcacttg gatttgacat aaaaataaat ttaaaaaaag
     2641 tattcagtat ggggaaaaaa tcagaaaagt atattaatga gaattgtaat gataaagatt
     2701 ttataaaaaa gtttatgtcg attaaaaaag gagattattt tttatataaa gcatatcaaa
     2761 atatagaaga aaaagttaaa tgtaatgtgt ctattggcat agttgaagaa gattttgaaa
     2821 atggatatga attaagtcat attttagggc atacactacc agtaaaatgg atgcataatt
     2881 ttgatacagt ttcaaattgt aaagtatata caagtgaatt ttataaatta aataataatg
     2941 atattagctc atatttagaa attattaagt cacaagataa gaaattattg agaaaaagaa
     3001 tagaagaaag tgaaaataat aaatggtatt taaaaaacta ttattataaa aatgtagata
     3061 agtgtgtaga tgatgaagaa ttatttattc agcccattaa tctagaacaa ttagaaagcc
     3121 taaaaaatat aaagacagat gattatattt tattgtataa tagcgagaaa gtaatttgtg
     3181 aaaatgacca tacaatgaag agtctatttg gcgtatgtca agttatggaa gggttcgata
     3241 aagagaaagt aagtgctttg gaaggtggat atttttttcc tgttagatgg atagagtatg
     3301 aaaaggagaa tatagacgga aatgcatggg ttatatttga aaataatagt atagttgaaa
     3361 agtatgtaaa aagatttact aataaaatgc cattaaatac tatactttat ggaccaccag
     3421 gaactggtaa aacatacaat acaaaacaga tggtttacaa tctagtaagt gaaaccagtc
     3481 taagaaacaa atactataca ggttacttag attataaaaa caatgtagaa ttttgtacat
     3541 ttcatcaatc atatggatat gaggaattta tagagggtct taagtctgat ggtgaaggca
     3601 attttaagcc agaagatggg atattaaagg atatatctat agaagcatgt tataatgcat
     3661 tgaaatttga aaaaaagatg agctttgagt tggaaaaaga tagcttgact ttagaaacta
     3721 taaaagacag gaaaaagaaa ttagtattag aaaatataga atatagtgag agttttaatt
     3781 ttgaagaatc agaaaactat gttttaataa tagatgaaat taatagaggg aatgtatcta
     3841 aaatatttgg tgagctaata acattattgg aagatgataa aagacttact aaatcaaatc
     3901 aaactattgt caaactacca tattcaaagg aaaaattttc tctacctcca aatttgtata
     3961 tagttggaac tatgaacaca tcagataaat caatagcact tttagatata gctttaagaa
     4021 gaaggtttaa ttttgaagaa attatgccag attatgattt attgtctatt gtagatggta
     4081 tagatattag aaaaatgcta tatacaatca ataaaaggat agagttttta tatgatagag
     4141 atcatgttat tggacaagct tatctacttg ctgtagaaaa tattaatgat atagtagtag
     4201 tatttagaag taaaataata ccacttcttc aagagtattt ttattatgat tcagaaaaag
     4261 taggtttgat tttaggagga attggaagaa atgaaaaaga taaatacatt gtttataaag
     4321 aaaaacttat agcttctgag ttatttaaaa ataataatgc ctcaataatg gagagtaaag
     4381 ttaactattg tataaaacaa aacatatcta gtgaggagct aaaaaatata tatgagtaaa
     4441 aatatatatg ttaaggaaac ctatgaatgg ataagggtag gtaatggtga aaatgagctt
     4501 acagaaattg aatatgaaaa attgcttaaa tatctagaga acaataatga tgtattaaaa
     4561 agtaatataa tagatattaa atacaaaaaa cttagattta taaactatgt aggcatcata
     4621 tgttttgaaa atgtaatact tgaaatactt cctaaattgt cattaagtga taatcttgta
     4681 aaagatagag agatactatt acagatgctt tctatatgta ataaaattcc aattacaatg
     4741 aatgaaaaaa taagattgag tttgaaaaat tataatctct taaatttttt tgttatgtat
     4801 ttcattgaga gtatgcaaac acagatgaaa aaaggtattt attttgaata tataaataaa
     4861 atagagaact taaatgttat gaggggtaaa atattattaa gtacatatgc aaaagaaaaa
     4921 ggcatttctc caatgaaaat aagatgtgaa tatgatgagt atagtgaaaa taatttcttg
     4981 aatcaagttc ttaaaaaagc atgtatatct attttatgta gaataaatga caattcaatt
     5041 caaggcaaga ttaaaaaaat tttaagctat tttcaaaatg tagatttaat ttatatagat
     5101 agaaaaaaac tattggatta taaattttat aaaaacaatg atagatttaa agattgttat
     5161 ttactagcta gacttctact tttaaatttg tctatggata atagtcaaaa taatcaagag
     5221 gcattttcta tattatttga aataaatact ttgtatgaag aatatatagg aattttaatc
     5281 aaaagtatat gggacaattc atttagagaa acatatatac aagataagag taaatttctt
     5341 ttgaaaaatg aacaaacagg taagaaaaac tttaatttaa gaccggatat agttttatat
     5401 gacttaaaaa atgaatatga aattataatt gacactaaat ggaaagctat tgaagtagat
     5461 tcaaatgttt tttataggtc aagtgatata taccagatgt atgcatatat aacagcatat
     5521 gaaaatgcaa aaagatgtat attattatat ccttgtattc aaaaagataa gaattatagt
     5581 tcctggaaat tatcagaatc atttaagggt aagtttatag aagcaaaaac tgtgaggtta
     5641 gatgat
//
LOCUS       DAELJO010000225         5516 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELJO010000225 DAELJO010000000
VERSION     DAELJO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.227"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..566
                     /locus_tag="KPB88_003436"
     CDS             <1..566
                     /locus_tag="KPB88_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055674.1"
                     /translation="GEMKNHIKDLDVESISGKKCVIDRLISKYKDFYEKLDNKFCVLK
                     EIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYIEERKVHLKEGNVRAYF
                     IRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMVYNLSKELLLEGKVPCL
                     FYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(663..1487)
                     /gene="pdaA"
                     /locus_tag="KPB88_003437"
     CDS             complement(663..1487)
                     /gene="pdaA"
                     /locus_tag="KPB88_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0055675.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1708..4827
                     /locus_tag="KPB88_003438"
                     /pseudo
     CDS             1708..4827
                     /locus_tag="KPB88_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            4845..5444
                     /locus_tag="KPB88_003439"
     CDS             4845..5444
                     /locus_tag="KPB88_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG0055676.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
BASE COUNT         2280 a          480 c          921 g         1835 t
ORIGIN      
        1 taggagaaat gaagaatcat ataaaagatt tagatgtaga aagcataagt ggaaaaaaat
       61 gtgtaataga tagactaata agtaaataca aagattttta tgaaaaacta gataataaat
      121 tttgtgtatt aaaagaaata aaagaaatag atttttccaa catgaaggag tataaaatag
      181 aaaatgctca ggaaaaagat attgatgaaa taggaaaact tttaaatagg tcagactata
      241 aagtaagtaa aaattacata gaagaaagaa aagttcattt aaaagaggga aatgtaagag
      301 catattttat tagaaatgat gatactatga ttagcactgt gtcaactggt atggaaacta
      361 gttttttggc aatggtggtc tctgtaagta cagataaaag atatagggga aaaggacttg
      421 caagttatat ggtttacaat ttgagtaaag aattgttgtt agaaggaaaa gttccatgtc
      481 ttttttataa taatgatgta gcaggtaaaa tatatcataa tataggatac aaagagataa
      541 atgaatggac aattcttttt aaatagctat aaaaaataaa aaactctatc gagaattaaa
      601 gtatttataa ataactatat ttttactata attcatgata gagttttttt aaatttatga
      661 tactattttt tattatttaa ataatcaagt gttttaagtt tataaccttc tttttcccaa
      721 tgtgtaagaa cctcatctaa tatctcagta tttgttgatg aatttggatg cagtaaaact
      781 attgcaccag gatgagttct tgaataaatt ttgtttttgg caaattcatg agtaggttgt
      841 ttcttttcat accaatctac ataagcaaaa ctccaaaata ttgatttata tcctaaatct
      901 tgtgtatatt ttaaagattg ttcgctaaat ttacccattg gaggtctaaa atacttaggc
      961 atttcttttc cagtaacatc tttgtatgct ttttctacac ttgtaatttc ttctttgaat
     1021 ttttcaaaat ctgtaatgcc tgccattgat ggatggcttt ttgaatgatt acaaactaaa
     1081 tgtccttctt tttccatacg ctttattaac tctggattgc tttttatata actttcaact
     1141 acgaaaaatt gcgctttagc attgtgtttt ttaagcgtat ctaataattt tggagtgttt
     1201 ccactttcat atccagcatc aaatgataga tatataactt tttcattagg atttcctaca
     1261 taataagctc cgtatttttt aaaaaaatca gcttctttta taggtgatgg ctgtttacca
     1321 tcttccctag ggttaaaata ccaatcatat tcatgtgtgt cgagtgttgg actagaaatt
     1381 tgagtttttg ttttatctaa tgatttaaaa tttatactag caattccaaa aagtattaga
     1441 gcaaatgctc ctatcataat gtattttttt aggctatctt ttttcacaat ttcacctctt
     1501 atttaaaatt taaagtatat ttcttataat atagtttgaa tatttatagt taaaaatatt
     1561 cacatataca aataataaat gaaagaagtt tactgtaaga tataaagttg atattaatta
     1621 tcttaaataa agtgtatata aatgataaaa tataaagaga aatgattgta aataagttag
     1681 gctggtttaa cttgaaggga tataaaaatg gataatataa taagtgtatt gaatgatgtt
     1741 gtttttttag atatagaagt tagtggatta gattgtttaa attcagaaat tttagaagtt
     1801 ggagctgtca aggttaaaga ctggaaaata tatacctatg aatcattaat aaaaaacaaa
     1861 tttgaggtac ctatagaagt attctcagta tgtaaaaatt tagataaaaa tgatttagaa
     1921 atagctaatg aaatagaact ggtggaagat agacttgtaa attttgtaga agatagtttc
     1981 attatatgtc atgatttaag tctgaaaaaa aagttttttg agtatcatat gccaaagtta
     2041 aaaaacaaat ttattgattt aattgaatta gcagttattt tagaaccata tcataaatat
     2101 tatagcttag aatacttaaa aaatacttta acaaattgta attctaaagt ggaaaataga
     2161 gctttatcag atgctattga tataataaat attgtaaatt gccttttagt aaaatttaat
     2221 aactatgaaa aaactacttt ggagccattg agttttaaaa taaattcata tcttaaaaaa
     2281 tttaatcttc caacatggga atggagcaaa tttttagaag aggcaaatta tgatttatca
     2341 aataatataa atataaaaaa agaatataat atatttgact caaaagaaga aaagaagaag
     2401 gaaagagaaa ccttaaaaat tttaaatgaa gaagaaaaaa attatgaaga attattaaaa
     2461 tacaaaacta tttgggagaa taaagaagga tttacatatg aatatagacc tggtcaatat
     2521 gagcttacta aaacaataag agagttgttt agaaatagcg aagatgaaga aaaaattgca
     2581 tgtatagagg caccaactgg tataggtaaa agtgtaggat atttattgcc tgcaatatta
     2641 gaggctagaa ttaataaaaa aagattatta atttctactg acacaaagga attgcagata
     2701 caattaataa ataaagatat tccaaatgtg ttagattctt tgggtctaaa tggaaaagta
     2761 agttatggat atataaaggg aaaaaataac tatatttgta tagatagact ggaagcatat
     2821 atagatgatt atgagtctca aaaccctact aaaggagaaa ttttatcttt aatatttttg
     2881 aaaagattag ttgaaggtgg caagtatgga gatatagaag aaataaatta tttagtgttt
     2941 gacaacttca aggaaataag tactcactta agaaatgtaa gttgtgaccc aaatatgtgc
     3001 agacctaaaa aatgcaaaaa agattgttta tataaaaaca gaatagaaga attaaaagaa
     3061 gaacatataa cagtagtaaa tcattcacta ttagctaaat ggccgtataa ggatgaaaaa
     3121 cctttagaaa atataatagt agatgaagca cataatttga ctgaaaaagg atatgatttc
     3181 ttttcaagta ttataaattc aaagtcttta aggtacttat tacaagaaat ttatccttac
     3241 gagtttattc aaaatagtag ctttatatat aaaaaatata gtagaaatat gagaaaaata
     3301 aaggcttttg ataaatttta taatgtatta aaaattggaa gagaagataa acaaaaaatt
     3361 gctagaagca taaatctcat tattgaagag atagattcaa tattaaattt tggtaattgt
     3421 aatgaatata ataatgtaag taattataat ttaagatggg aactgaattt gtaaatagat
     3481 gaaattgtgg gaaaactaaa aaaggatggt atagatactg aaataagtta tagagcctat
     3541 agtgaaaaga taaagttgag ttgtgagaaa ataataaaaa atttagtttc aataataata
     3601 ataatttata ggaatattga tgatgatagt atagataaag aagcagatat atataaattt
     3661 ggaaaggcaa agacaagaga tttagaggat ataaagatta tatttgaaat ctttttagag
     3721 tatgatgaaa aggatgatta tgcaaggata gttgaaattg ataagaatta taatgacttt
     3781 gaatttcgag tagtaccatt aaaaatagca gatttatttg aagaaaatat tttatctcaa
     3841 ttagaaaaag gaatattttt atcagcaact ttgagtttat ctgaaagtat gtcttatttt
     3901 aaaaatacat taggaataga cagagttaag aatgtggaaa agattataga accaattttt
     3961 gattataaaa atagagtctc tgtagttgga ttttctgata tttgtgagta cagaaattct
     4021 gaatttccaa atgagatgag taaaattata tctaatatat caaagattac agaaggtcat
     4081 acattagctc tttttaatag taaagataga caagaaaaaa cttatgaaat attgaaaaaa
     4141 tacttacata gttttaactt agaaatttat gctgataaaa aggggattag acaccttaac
     4201 gatttaaata gaaaatgtgt agtacttggc tcaaaaggat gttttgaagg cgtagacatt
     4261 ccaggggatg gtttggtttg tgtaacttta gacaagcttc cgaatctaaa tccaaaagac
     4321 cctttgtatt ttactataat gaagaagtat ggtatagact actatactat aaattatcct
     4381 caaatgacca taaaggtaaa gcaagctatg gggagaattt taagaagcaa atatgactat
     4441 ggatgttttg tgatttttga tgtaggaact aatataagtg ttttaaagag attagagaaa
     4501 gatttacacg attgtaaaat ttcaaaggtt aatagcaatg agttttatac ttatataagg
     4561 cgacatttaa ataaatctag aagtttaata ctaaaatctg ttatatttga tactataaaa
     4621 gcgttgaatg ttgatgctaa aatggataac aatgatgttg ataaggatat tataaaaaaa
     4681 gatataaatg aaaatataag acaaagagct gtaaaaggag aagtgtacca tattgatatt
     4741 ataaaaaaag atatgaaagt aaaatatttt gatagaaact atcttataaa tttagatata
     4801 tttatgagag aagaagataa aaattaagag tgggagtgat atatttggca aaacaaaagt
     4861 tttatgctgt gaaaaaaggt aaaaatatag gtgtatataa tacatgggat gagtgcaaaa
     4921 aacaagtaaa tggattctca ggagctgaat ataagagttt ttctactttt caagaagcta
     4981 aagaatatat tgatgggtca gaaaaattga gttttcaaga agataaggag tttatagaag
     5041 catatgtaga tggaagttat gaacattcag taaaaatgta tggttcgggt gttgtgattt
     5101 taaaaaataa tgaagttata aaaacttata gtgaaaaagg aaaagagaaa actttagtaa
     5161 gtatgagaaa tgtagctgga gagatagagg cttctaaaat tgcaatgcag tactgtattg
     5221 acaataatgt tcaaaacttg attttatact ttgactatga agggattgaa aagtggtgta
     5281 ctggagtttg gaaaacaaat aaagaaggaa ctatagcata taaaaatttc tatgacagta
     5341 ttaagaataa gcttaatgta aaatttacaa aggttaaagc tcattctgga aataagtata
     5401 atgaggaagc tgataaatta gctaaaaaag ctattggtgt ataataaaaa aatatttata
     5461 cataattaca tggtataaga gtatatctaa attcctactt tataattaaa caactt
//
LOCUS       DAELJO010000226         5503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELJO010000226 DAELJO010000000
VERSION     DAELJO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.228"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     misc_binding    79..313
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            363..1382
                     /gene="pheS"
                     /locus_tag="KPB88_003440"
     CDS             363..1382
                     /gene="pheS"
                     /locus_tag="KPB88_003440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG0055677.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1399..3792
                     /locus_tag="KPB88_003441"
     CDS             1399..3792
                     /locus_tag="KPB88_003441"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG0055678.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3817..4407
                     /gene="zapA"
                     /locus_tag="KPB88_003442"
     CDS             3817..4407
                     /gene="zapA"
                     /locus_tag="KPB88_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG0055679.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            4688..5371
                     /locus_tag="KPB88_003443"
     CDS             4688..5371
                     /locus_tag="KPB88_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG0055680.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         2156 a          600 c         1077 g         1670 t
ORIGIN      
        1 atgtattgga tatatattaa aaatagtcac ataatttgaa tggaaatgat ataatactaa
       61 aataaacaat ataatattgt aaatgcaatg aaagaggaaa gtattttgat taaacttagt
      121 aaagagataa acacctaggc tgggagtgtt ttctaagagg tcatgagaag ttccctctgg
      181 agtaacagag ctgaaatttt acagtaggct ttgacgtcaa aaacgcgtta agtttgttag
      241 aggtggtttg atgattttta aattgttaaa ctactagggt ggtaccgcga aactatagcc
      301 tttcgtccct agacagggat tgaggggctt tttttataca aaaaaacgaa agggtgatat
      361 gtgtgcaaga aaaattactt gctttacgtg aagcagcttt ggctgaaata aaagaagcac
      421 aaagcataga aagtgtagaa agtttaagag ttaagtactt aggaaaaaaa ggtgagataa
      481 ctgccatact taaagaaatg ggtaaattat ctgctgaaga aagaccagta gttggtaagg
      541 ttgccaatga ggtaagagaa aacattgaac ttagcataaa ttctaaaaaa gaagaaataa
      601 atgctattga aaaagaaaga aaattaaaag aggaagtgat agatgttact caaccaggaa
      661 aagttttaaa ggttgggaag aagcatccaa taactcaaat tatagatgaa gtaacagata
      721 tatttatcgg aatgggattc tctatagcag aagggccaga agttgagact gttgaaaaca
      781 actttgacgc attaaacgct cctaaagacc atccatcaag agatatgagc gatacattct
      841 atatcaatga tggggtatta cttagaactc aaacatctcc agttcaagta agaactatga
      901 gaagtcaaga gttaccaata aaagtaattg caccaggtag atgttttagg tcagactcgc
      961 cagatgctac acactcacca atgttccatc agatagaagg gcttgttgtt ggaaaagatg
     1021 ttactatggc agaatttaaa ggaactatgg atatcttcgt tgaaaaattg tttggttctg
     1081 atatcaaaac taagtttaga cctcacaact tcccatttac agaaccaagt gcagaggttg
     1141 atgttacttg tttcaaatgt ggtggtaaag gttgcccaat gtgtaaatat gaaggttgga
     1201 tagaaatatt aggttcaggt atggttcatc caaatgtgct tagaaattgt ggaatagacc
     1261 cagaagttta cagtggattt gcatttggag ttggggttga aagacttgca atgcttaaat
     1321 acgaaataga tgatattaga ttattattcg aaaatgatat gagattctta aatcaatttt
     1381 aattaggagg gtatgtagat gttagtatct ttaaaatggc ttagagacta tgttgatata
     1441 gacatggatg taaaagagtt cgctgataaa atgacaatga caggaactaa agttgaaaca
     1501 atagattatt atggtgaaga aatagaaaat atattggttg gaaagatttt agaaataaaa
     1561 caacatccaa atgctgataa gttggttgta accaaagtag atattggaga taaagttgtt
     1621 caaatagtta caggagctac aaatatatca gaaggagatt atattccagt agctgtaaat
     1681 ggttctaagt tacctggagg agttgaaatc aaacagactg atttcagagg tgaattatca
     1741 gatggtatga tgtgttcagc agctgaacta ggtatagatg aacattacat tgaggagtat
     1801 aaaagaggtg gtatatatat tttagaccac gaagattctt atgaattagg aaaagatata
     1861 aaagatgttt taggattaaa agatgcttta atagattttg aattaacttc aaacagacct
     1921 gattgtaaat gcatgatggg tatagctaga gaagcagctg caactatagg aacaaaagta
     1981 aaatatcctg aaatcgaagt aaaagaaagt gacgaagaga tagatttcaa agttgagata
     2041 gataatccag atttatgtag aagatatgtt gctagaatgg ttacagatgt aaaaatagaa
     2101 ccttctccat attggatgca aagaagactt acagaagcag gagtaagacc tataagtaac
     2161 atagtcgata taacaaactt cgtaatgtta gagcttggtc aaccacttca tgcttttgat
     2221 ataaatcaag tagagactgg aagaatagta gtaagaaatg ctaaagatgg agagaaactt
     2281 gtaacattag atgatgttga gagaacatta gacaaagata tgctagttat aacaaatgga
     2341 gaaaaatcac ttggtttagc tggtgtaatg ggtggtgcta actcagaaat aacttctaat
     2401 acgaagactg tactttttga aagtgccaat ttcaaaccag aaaacataag aatgacagct
     2461 aaaaaagttg gtattaggtc agaagcatct tcaagaaatg aaaaagactt agaccctaat
     2521 cttgcagaga tagcagcaaa tagagctgca cagcttgttg aaatgttagg agcaggaaaa
     2581 gttttaaaag gtgttgtaga tgtatatcca aataaaccag aacctaaaaa attggtagta
     2641 aatcctcaaa gaattaacca cctattaggt gtagatgtac caatggaaca gtttgtagga
     2701 attttagaat cattagagtt taaatgtaat ttggtagcta atgataaatt agaaatagat
     2761 gtaccaagct ttagaacaga tatggaacaa gaagctgatg tatgggaaga aatagctaga
     2821 atttatggat ttgagaatat accttctgta caattagaag gaaatacgac agcaggaatt
     2881 aaaacttcaa aacaaaaatt catggatgct ttaaaagata attcaactgc tgtaggatta
     2941 aatgaaatat taacatattc atttgtaagc cctaagggag tagataagat aagagtacca
     3001 gaaggtaatg caaagagaaa ttttgttaaa ttacttaacc cattaggaga agaaacttct
     3061 gtaatgagaa ctactttaat accaaatatg ttagatgttt tatcaactaa tgtatctcat
     3121 aaaatagaag aagtatctgc atttgagtgt ggacatatat tcatcccaca agattcagaa
     3181 ttacctaagg aagaaaatag aatgtgtgta ggtatgtatg gtaaagatgt tgacttcttt
     3241 gcactaaaag gaactataga aactatactt gtaaatgtcg gatttaaagg ttatgaaatt
     3301 gaacctcaag ataataatac taccttccac cctggtagat gtgctaaaat agtgtataat
     3361 aataaatatg taggtacatt aggtgaattg catccagatg ttatagaaaa ctacaatcta
     3421 ggtcaaagag tttatgtcgc tgaaatagat attgactttg tatttgataa ttctgataga
     3481 acaaagaatt atgtgccatt accaaaatat ccatctacat caagagatat agctcttatt
     3541 gttaaagatg atgtatttgt aaaacaaata gaagatataa taaaggaaaa tggtcaagga
     3601 ttagtagaaa gctacaagtt atttgatgtt tacaaaggtt ctcaaataga agctggatac
     3661 aagtcaattg catattcaat aacttataga agtaaagata aaactctaac agatgaagat
     3721 gtagctaagg tgcatgataa gatattaagt gaattaagcg aaaaattaaa tgctaactta
     3781 agatcaaatt aacaactaat gtcgggggag gttaggatga acaaagtaat ggttaaaatc
     3841 catggcgctg aatatccaat ggttggagac aaatcagaga agtttatgat tagtattgct
     3901 gattttgtag acaaagaaat ggataaaata acacgtcaaa acccgaaatt gagcttatca
     3961 gttgctgcga tattaactgc attaaatata tcagaccttt tatttgaatg ttcagatgag
     4021 aatgagaagt tgattaaagc gaatgaagaa ttgagtaaga aggttggagc atctaatgaa
     4081 gagcttcaat tagaaatcaa atctttaaag cttacgatag cagaaaaaga agcagaaaat
     4141 cgtgaagctg aaactaagat gaaagagtta attgagatta tagaaaataa aaagcaagaa
     4201 atattcgaac ttagtaatac gacagaaggt tctagagcag agttagatgc ttataagaac
     4261 aagattgagg agttaagtac tcaacttgaa gaagcaaatg aaagagctac tatagctgaa
     4321 aacttagctt ctgagtttca aaacaaagct tatgatttac agttaaaatg tacagggtta
     4381 aataatgatg caaaaaatgt ggaataaact aaaagatttg ctgtctcttg ttttttgaga
     4441 cagcttcttt taatgtataa atataaatgc tatataaata tagataatgt ttaaatgtaa
     4501 ataaatataa atgctatata gatatagata atgtttaaat ataaataaat atgaatgata
     4561 tataaataca aataatgtat aaatatagac aatatttaaa tataaataat ttttttatat
     4621 caatgaaaaa tagaatctaa aatgattttg attttgtatc tatcattaga aagcgagagg
     4681 tttatatatg acaagcaaga gtcagatgtc tgaatcattc tttttatgtt ctttgttggc
     4741 aataacagga ggatttttgg atatttatac ttatgtggta agaggtaaag tctttgctaa
     4801 tgcacaaact ggaaatatag ttttgtttgg gcttaatata gcagaaggta atgtaaagga
     4861 atcattttat tatttgattc ctattttggc atttatgtta ggtgtattta tagccgagaa
     4921 gataagaaag tattttacaa ataataagac aaagattcat tggcgtcagg ttatcattat
     4981 aattgaaatg attacaatac ttattgtttc atttattcct agaggaagat ttaatatgtt
     5041 tgtaaatgtt gctatttctt ttatttgttc aatgcaagta gagagtttta gaaaagtaaa
     5101 tgggaatagt tttgcaacga caatgtgtac tggaaattta agaagtgcta cagaaacttt
     5161 atttcattat atacatacta agaatatttt tatgataaaa aagagtttac agtattatgc
     5221 tattattata ttctttatat ctggagctat tttaggaact ttttttacaa aaattttcgt
     5281 ggaaaaatca atactaatat gtttttttat tttggcagtg gtctttggaa ttatgtttat
     5341 caataaagat gattcattta atgaaaatta actttctgta aatttacaaa aaaaaaatga
     5401 agctgttatt tgatatactt tatatatagc aaatattgta tagattaatt aaagaattta
     5461 aagtatagta agcagtgtat aaattaatta aaatgtctta agt
//
LOCUS       DAELJO010000227         5451 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELJO010000227 DAELJO010000000
VERSION     DAELJO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.229"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            66..2075
                     /locus_tag="KPB88_003444"
     CDS             66..2075
                     /locus_tag="KPB88_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0055681.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLKDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            2319..3065
                     /locus_tag="KPB88_003445"
     CDS             2319..3065
                     /locus_tag="KPB88_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0055682.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDEEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLEAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQKHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            3106..4425
                     /locus_tag="KPB88_003446"
     CDS             3106..4425
                     /locus_tag="KPB88_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0055683.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            5090..5320
                     /locus_tag="KPB88_003447"
     CDS             5090..5320
                     /locus_tag="KPB88_003447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055684.1"
                     /translation="MKKYTKDLHIMSERNSYSKTDNDATFMRMKEDHMKNGQLKPAYN
                     IQFGVDSEYIVLVSSGHQPTDTITLIPFLKSI"
BASE COUNT         2211 a          536 c          985 g         1719 t
ORIGIN      
        1 ctattaaagt gaaaatcaga tgtatattat aaaagagtag agtatatttt aaaaggagta
       61 atcaaatgaa aacaatagca gtagtaactg atgggttgtc taaattagaa tgtttcttaa
      121 ataaaaatct agaaatgata tttaaagata aagtaatgat aaaaaattat tatttaaata
      181 aaataacaga gaaggaatta ataaaagatg acataatatt ggtaatgata gatgatagag
      241 tattaaaaat aaaaaaatat gtagaagatg tctcaaagat aataactata aataggagta
      301 taaagcaaaa ggatatatat aagttatttt cattgccaga aggaatagat gtattagtag
      361 taaacgataa taagcatact gtattggaaa caatatctaa tttatacaat ataggaataa
      421 accatttgaa ttttatacca tacaatccca atgaagaata tagaaatatt aagatagcta
      481 taacgccagg agagtcaagt ttagttccaa aatatataga agaagttatt gatttaggtc
      541 ataggtatat tgatagttca acgtttattc aaataatagc aaaactcaaa ctggatgata
      601 aagaaattac tgagaggcta attaagtata cagatgaaat aattagttta tattcaggaa
      661 taaatactaa atacaaagaa cttactataa aagcagatga attaaattct ataataaatt
      721 tatcaaactt aggtatggca atggtatcag ataagggaaa cataattatt tgcaataata
      781 gtttaaagga tatactagat attcaaagta atattatagg gaaaaatata gatgaattag
      841 agaatgagaa tattaaaaag atttttagtt tgagtaaggc acatgatgaa gttataaaat
      901 ttaataataa gtacttaaac gtaaataagt ataatataga gagttttgga agggttactg
      961 gatattattt ttgtattcaa gaaataactt acataagaaa attggagcaa aacctatcaa
     1021 agaagcttag agaaaaggga caagttgcta gatatacttt tgatgatata aaaacagttt
     1081 caacgaagat ggaaagaact aagaaattag gtatgaaaat agctgaatct gattatacaa
     1141 ttcttataac gggagaaagt ggaacgggaa aagaactcat ggctcagtct attcataatg
     1201 catcaccaag acatagtcag ccatttatag caataaactg tgcagctatg ccagaaaatt
     1261 tacttgaaag tgaacttttt ggatatgaag aaggtgcttt tacaggagct ttaaaaggag
     1321 gtaaaaaagg attatttgaa caggctaaca atggaactat atttttggat gaaataggag
     1381 acatgcctat atacttacaa agcaaattac ttagagtatt acaagagaat caagtaatga
     1441 gggttggagg agaaaatgta atagatatag atgttagagt aattgcggct acaaataaaa
     1501 atttattaga aatgataaaa ggagatagat ttagagcaga tttatactat aggataaatg
     1561 tattgcccat aaatatacca cctttaagag agcgaaaaga agatatagaa gttatgttaa
     1621 agcattttat gaaaaggaag agagcaatta gtcaagatgt tcaagacatt attaatgcat
     1681 atgaatggcc aggtaatata agagaattaa aaaatactgc tatgtatatt aacattatga
     1741 gttctgagga tgttatttta ataaatgatt tacctcataa cttattaaat ataactgaag
     1801 attttagtaa agaaataagt atattaaagg atagaacatc tatagagaaa gtaaagttag
     1861 taatggagtg tttaaaaaat gccaataatc ttaataaaaa tataggtaga aatggtattg
     1921 ttaattcaat aaatgaatta ggctacaata taacagaagg tgaggtaagg ggaatactta
     1981 atatgttaaa agagctggat ttgataatat gtgaatctgg tagaaaggga agtgaattaa
     2041 atagaagggg aaaaaatata ttaaacagta tttaataggt tgataaaaag gaaaatctaa
     2101 atgcctattt tacctattag ttgtacttaa aacaatttaa aaaagtcaaa ctaaacagga
     2161 ttaaaataaa atataagtaa ataaagttat ttatattact cgtattcaaa aaaagtattt
     2221 ctaaaataaa ttataatgtt caaataaata aaatcttggt atgctacttg ctctttaaga
     2281 gaagtagcat tttttattgt tcacaaataa gttaaggggt gaagaaattg gttaaaaaga
     2341 aatatccaat aataggaata tcaggtaatt tactaattga tgaaggagga atgtttcctg
     2401 gctatgaaag ggcttatgta aataatgatt atatacaatc agtagttatg tgtaaagcta
     2461 taccatatat tgtaccgatt gtatatgatg aggagattat aaaggagcag gtatctaata
     2521 tagatgcgct tatactatct gggggacaag atgtaaatcc actcatatgg aaagaagaac
     2581 ctcataataa gttggaggca atatcaccaa agagagatag ttttgatatg aaattattaa
     2641 aacatgcttt agatatgaaa aaaccagttt taggtatatg tagaggagaa caaattataa
     2701 atgtaactga aggaggtagt ttgtatcaag atttatcttt aattgaaggt gcttatatta
     2761 aacacaatca gaaacatcta tctaatgtac ctacacatac agtacagata aaggagggga
     2821 caaaattata tgaaatacta ggagaaaaag aagttttagt taatagtttt catcacttag
     2881 ttgtcaacaa agtagcacca ggatatattg tttcagctac atctaaagac ggtttaattg
     2941 aagctataga aaaggaagga agtgagtttg taatagggat acaatggcat ccagaaatga
     3001 tgactagaga ttatgataat atgaaaaaaa tatttatggc aatagttaaa gaagcatcta
     3061 aataaaaaat ataaatatat taagttggct ttggggggaa taacaatgga aaaaggcatt
     3121 aagttaggtt tttggagtat tgtactatta ggaatcaatg caataatagg gtcaggtata
     3181 ttcgggttac ctggtgatgc ttatacagat ataggtccag caagtatatt agtattagtt
     3241 ttttgtatgc ttttagctgt ttctattgct ttatgttttg ctgaagcagg tagttggttt
     3301 gatactgatg gagggcccta tctttatgca aaagaggcat ttggagattt tgttggattt
     3361 gaagttggat ttatgaagtg gatagttagt atgattgctt gggctactat ggcaaatttc
     3421 tttgctgtaa cactatcatc tgtatggcca caagcagcag aaccacttac taaaaatata
     3481 ataattggta tattagttgt agggcttggg attataaatt ttatgggaat gaaacaatct
     3541 aagcacttaa ataatatcat gactatagga aaattattac caatagtttt atttatagct
     3601 gtaggattat tctttataaa gggttctaat tttaccccat ttgtaataat acaaaaaggt
     3661 caaagtgcgt catcagcttt tgtagcagta tctataactt tattttatgc ttttacagga
     3721 tttgaatctt tagctgttgc agcaaaagat atggagaacc ctaagaaaaa tgtacctaaa
     3781 gcattggtaa tggtaatgtt tgtagtatca gtaatatata tgcttatact gggaataagt
     3841 ataggggtgt tgggtaatgg cttagcaggg tcagctactc ctgttgcaga tgctgccatt
     3901 aagatgttag gtcctatagg cgggtatata attacaatag gaactatagt atctgttgga
     3961 ggtataaata ttgcatcatc catatttaca ccaaggtctg ctgctgcttt ggttgaacaa
     4021 ggattaatgc ctaagtctat aagaaaaact aataaaaatg gagctcctta tatagcaatt
     4081 atagtatctg tcataggaac tttactaata gcatggtcgg gttcattcac aacattatct
     4141 caaataagtg ttgtatctag atttattcaa tatattccta catgtctagc tgtactaata
     4201 ttgagaaaga agtatgctgg taaagatgta aattttagaa tacctggagg agcaattata
     4261 ccaatatttg cagttattat aagtatatta ttactgataa aagcaggtat tgatgaacct
     4321 atgaagatag tttggggatt aggtgggatg ataattgtag ttcctatata tttctatatg
     4381 actaaagtat attcaaagaa atataatgat gtagaagtca aataattatt gtaatctata
     4441 gagattctat aaatattact ttgaatttag ggaaatgggg ctgtatcact agaaaatagt
     4501 gctacaccct ttattataca agaatcccaa gttcaaaaga gtttgggagt cttgtataat
     4561 aattgtgtgt taaaacaaaa tggtatatag tttaattata ctacttatgg cgataattat
     4621 caatttaaat taccactaag tattgattac atgattccaa aagatgattc tgtaaggtta
     4681 ttaagtcaaa ttattgagta gatgaattta gaaaaattat ataagactta tgctcgaatt
     4741 agaggaaata gagtaacccc tagacaactt ttgaaaatta taatatatac aaacattaac
     4801 tgtatttatt cttcaagaga aatagagtca tctgctaata gatatacttt tgtttgaaaa
     4861 aagctataaa taaaaatatg gctaaaatga tgaataatat atctgacttc atattaaaat
     4921 gtgagattga ttttggtatt aaaataattt ataaagataa aattaaaatc catcatttaa
     4981 ggaaattttt aaagaaggtt aaaaaatatc aaaagaatca aatataacat ttgttcatgg
     5041 aaagggaaag aacaaatcta tagagcaatt aaatgattgt attgatagat tgaaaaaata
     5101 tactaaagac cttcatataa tgagtgaacg aaatagttat tctaaaacag ataatgacgc
     5161 aacttttatg agaatgaaag aagatcatat gaaaaatggg caattgaaac cagcatataa
     5221 tatacaattt ggtgtagatt cagaatatat agttttggta agttcaggac atcaacccac
     5281 tgatactata acattaatac catttttaaa atctatttag ttccatacaa agtttaaata
     5341 tcctaaaata gtggatgatt ctgggtatga aagtgatgaa aactatacat ttttagagaa
     5401 atataaaata aataaaatat ttttaaaata gaatagtaaa agggaaaaca c
//
LOCUS       DAELJO010000228         5444 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELJO010000228 DAELJO010000000
VERSION     DAELJO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.230"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(204..1388)
                     /locus_tag="KPB88_003448"
     CDS             complement(204..1388)
                     /locus_tag="KPB88_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0055685.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KPB88_003449"
     CDS             1761..4112
                     /locus_tag="KPB88_003449"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG0055686.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KPB88_003450"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KPB88_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG0055687.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2137 a          612 c          865 g         1830 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttactatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggaa ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat ttgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaatcctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa atgaaaaaga tttaaaaaag tgttttaact
     5041 aaacacataa gtgttaaatt catgtataag tataggttag atacataaga aataattatg
     5101 taatatctat ctttgtaaaa aaatattaaa ttttgtaatt tgtggataaa tatgtttata
     5161 atgtggataa tatttaatgt aataagttaa agcaatatga atcaactgtt tataatgtgg
     5221 ataatattat aaatgtatga ataattgcag tatttaaaaa caaaaaaagt taaaaaagtt
     5281 gttgactcta aaagaaaaag ttggtatagt aatacttgtc ctcaagaaaa gggcaaacaa
     5341 ataaagcaca aagaactttg aaaattaaac agtaggttaa tttataaaac aagaaacaaa
     5401 ccataaagcc agatattttg ataacaatag tatctgagcc tgat
//
LOCUS       DAELJO010000229         5356 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELJO010000229 DAELJO010000000
VERSION     DAELJO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.231"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            3..401
                     /locus_tag="KPB88_003451"
     CDS             3..401
                     /locus_tag="KPB88_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG0055688.1"
                     /translation="MLKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSLS
                     WSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMINLFSL
                     NDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(466..2583)
                     /locus_tag="KPB88_003452"
     CDS             complement(466..2583)
                     /locus_tag="KPB88_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0055689.1"
                     /translation="MQISKKIISLLTVAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKSLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNRIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(2614..3321)
                     /locus_tag="KPB88_003453"
     CDS             complement(2614..3321)
                     /locus_tag="KPB88_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0055690.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(3428..5299)
                     /locus_tag="KPB88_003454"
     CDS             complement(3428..5299)
                     /locus_tag="KPB88_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG0055691.1"
                     /translation="MNKKNLSVIMAATMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTNNVVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
BASE COUNT         1543 a          976 c          571 g         2266 t
ORIGIN      
        1 tgatattaaa aaaacatcta gaaacaatat tatatctttt ttttggtgct ttaacagttt
       61 taataaacat tattacttac ctatttttta caagaattat taattttact tttttattat
      121 caaactctct atcttggtct atagcagttt tattttcata tataacaaac aaattttttg
      181 tattcaaaag tgaaaaaact gagtttttat ttctattaaa agaatttgta tcttttttat
      241 cttgtcgttt attttctggt tttatagaaa tattacttat gtatttaatg attaatttat
      301 tttctttaaa tgatatctta gtaaaaatag gcactaatat aattgtgata attttaaact
      361 tcgtcttcag caaggtagta gtttttaagc atagaaatta gcattattat atttatcttt
      421 attcagaata aggagttgcc taaaaaaaac aactccttat tagctttaag aaacaatttc
      481 tattttatct gttgctcccc atttttcaac tttattattt tctcctgatg ctaattctac
      541 ataaagtcca tttaccttat ttccttctaa aattatatca ctattagaag tacttttcca
      601 actggagtct atttcatctc ctacattcat agcttctggt tttgtagtct ttattctata
      661 gtaaacttta ttaccattgt atccagttac actaatagtt actttagaat tttcatcaac
      721 agaaacagtt gattttattc catttgctac tattttaaaa ccatcatctg tcgcaccagc
      781 tcttccccat cttgaaactt tctttcttgt ataatcaatt tcaacaactt ctatatatga
      841 tccattctta gcatttactt ttaatggttc cgtatcttca accaagtccc aatcatttat
      901 gttaattcta tcaccaaccc tcatagctga tggtttatat gacaaaactc tataataaat
      961 tttataattt gaatttttag gtttctgagg tacagttaac ataattcgat tattctcaag
     1021 aggagaacat gcacttatct ttggagataa tcctgctgct gtatcactat catcatcttt
     1081 atctgtatta ttagtgttag gtttattgcc acttattcct ggttctttat ttccactaac
     1141 agtatcaatc tttcccgaat ttcttatatt agtacttgaa gaatcattat ctatctttgt
     1201 tatagttcca tcatttctta tatttacatc ttttgcagaa ctaaatattg ttacaatcca
     1261 tatttgtcca ttattttcat tttcaatctt gcaaccatta tcatcagaaa tgtccatttg
     1321 atttatatat cctgaattag ttaaagttcc atcttttata tctttaagta tcaatgtttt
     1381 acttatatca cctgtattat tcaactctcc atttggcata tttatagtta ttgactgact
     1441 atattctcca tataaatcaa cctttatagc tttttcagtc tctaatttaa aagattcact
     1501 tatcttttta tcaggtctaa actttataat atcattagca tccactttct ttaaagcaga
     1561 atccaattct tcaattgttt caactgtata tttagtttct tcttgcatat ccaatatatc
     1621 tttatagaca ttttcatttc ctattccacc aacctgagta actgtactaa atatcttagt
     1681 atttagtaca tctttctgaa tagaatcaat tttatttcct actaatacca ctggagaatt
     1741 gttttttgca gccaacactc caactgatag tgaatctatt aaatcacttt gttttttcat
     1801 tccatccttg gttatatatg catttcttaa atctgtttct ttataaaatt cttttataac
     1861 attagcattt gtttcatttc tattatttcc tgatatcctc ttagaatttg gtaaactttt
     1921 ttctatttca ttagaaatgg aatttaatcc tccaataata taagaatttt ttatattttg
     1981 ggcttttata aatttattag ctacttcaac tccaaggttt gaatctgaaa gtataattgg
     2041 catgtaattt tgagctgcta ttgaaccaac acttacagca tctgctaatc ccttttctcc
     2101 atttactaca attatatcag aaatatcttt ttcattattc aatttttcag ccaattttaa
     2161 ggatgtttca tatctatcat tcccaaaaat tctttcaact gttattttct ctgattttaa
     2221 ttcattttct acatttacac ctagtgcatt tgaacctcct actaaaaata cattttttac
     2281 tccaagcctt tttagttctg attttgtcct aaaatccaat tctttacttc cagatagtaa
     2341 aataggtgca tctttcaatt tagcaaatgg agttgcagat aatgcatctg ctattgagtt
     2401 atcattcact aatacagcat tatcagatct taaccaaccg ttttggctta tttttattgc
     2461 tgtttcccat ctacctgaac ctattaaact gtctttatta gatgcattta ccacattact
     2521 aggtaaaaca attgataaaa atgctactgt taataaagat attatttttt ttgatatttg
     2581 cattaattat ctcctcctaa tttaaatata attttatgga gcatgtacat actgtgtttt
     2641 atttaattct aaatcatcaa aatgagattt tactgaatgg tatcctatag cataatgtcc
     2701 actttcagga ttccatttct tatatgcatt catcgcactt aataacttct cctcttgact
     2761 aggtaatacc ttccagactt ctacatttgg attaactttt ttaaaatcct tatagtcata
     2821 tggttctaca taaaatctta aatcttttat ttttccttct ctatataact ctagtgcagc
     2881 ttctcctaat gctctatgat cttcatgtat tccactagct ttaaacggag taacagtttt
     2941 tacttttgct tctggatatt cttctaagta tttaagtatt atttcctttg ccttttcttt
     3001 gcttaactca ccatcatgag ctctattatc ttctatatgt atattacttt cttttacacc
     3061 cattgcttca cagctatcct taaattcttc atctctatct tttgaaaatt cactttctga
     3121 taaagagtat ttatgaacat ctttaatatg ataagaacat gaccctccat catccaaaac
     3181 ttttcttatt atagatttac taccatctgt gcacaatatg acatgtgtat cacttttttc
     3241 aacatgttca attatagcac ttcccatact taaggtttca tcatcttgat gtggtgtaaa
     3301 aaataaagtt gcatctacca ctactacatc ctcctacttt ataaaatgtt ataatttaca
     3361 ataaatagca taattataga aaaagattcc tgtattaatt aaggaatctt ctccacagta
     3421 taagttatta ccaacctagt attttagcta tcttttgtat tactgtcaat tcaactccat
     3481 tacctatttg agttacttct gcaccaagat ttaatttttt actaagtgca ttcttttgtg
     3541 agtctgttat atcagtttta gctagaagta ttggaccttt acctgctgct agaggtcctg
     3601 ctgctaatgc atctactagc ataccattat ttccatatcc atcttttgct atatatagtt
     3661 tatctaattt gccataatat gtttctataa ccttagcatt ggtatcatgt cttgtttctc
     3721 cttcaactct ttgaactttt ctatctttat caatatcagc aagttgattt tcaatttgac
     3781 tagatacatt gttagaacca ccaactatgc caatctcttt tccatccatc aatttgatag
     3841 catctgctga tagatcattc cagccattta ctattatagg agccttaagt tcagcagctt
     3901 tagcagctat actcatagca tcagcctccc cttttgcgcc tactacaaat gcatctttaa
     3961 aagaacccat agcttttgct accgctacag aagtcgtatg tctatcatct ccagctagtc
     4021 tttcaacatt cttagttact gattctaatt gctttttagc agtatttgat actgctgatt
     4081 ctccacctac tatatatatt ttagctgatg ggcttttctt aattatatct tttatataat
     4141 ctaaagttac ttttggtatt tcattatcag atgctaacaa tataggtgct tttttagatt
     4201 gagcaagtgg tgtagctgct aatccgtcaa ctagtttatt tgaattaact ataactacat
     4261 tgttagtata ttttgtttgt tttgctatag ccactgcagt tgcgtatcta tcatctccag
     4321 ctatcaattt aacctttgaa ttaccttcta atgcagcctt agcatctttt aaatcttttt
     4381 cattagtact ttccacagta agctctattg cttttccaga tgcatcagta tacttaatag
     4441 taaatttata ttttcctgtt tctgaatctt ttgctgaaat atcactaatt gactttgatg
     4501 taccatttgc taccatatta cctttaacag ttaatctata tccatcatat aaatcttgtg
     4561 gtgaaactgt atttaggtca ctatttttta tagttatttc ggccaattgg acatcatcaa
     4621 tatttttagc tcctgctaca cctagtttat cgattttatt tactctaaat ccttctgcag
     4681 aagggtctaa atttgttcca gttgaatcaa ctggatttgc tgttaaatca actttttcat
     4741 caccaatacc tacatatata gttttagaag ttactgtttc tgttttatct cttaaagtta
     4801 taactaactt atctttagca ctatcatatg aagcttttac atctgcaact ttataaattc
     4861 cattagtttc agttttagat ttttctgtta tagagttagc ttcagcggaa aggtctgctg
     4921 aattttcata tttttcagtt gcttctgcaa ctacttcatt tgcactattt gttatatgcc
     4981 ctttatctgt tatagttaca ataagttctt tattttctcc taaagcattt actaatttct
     5041 ctaattcatc tatattagtt attattttta attttgataa agtctcacca acttttattt
     5101 catatattgg ttgcccaact tgagaaccac ctgtatactt ttgagacatt aaatctctaa
     5161 ctttcgaaac tagttctgta gcttctttct tagttattgt ttcttttttt acctgtgtag
     5221 tttctgcagc aaaaactgga gctactgatg tacttatcat tgtagcagcc ataattacag
     5281 aaagattttt tttattcatg gtattttccc cctaaaaaat tcaaattttt attatttttt
     5341 ccttatttac caatta
//
LOCUS       DAELJO010000230         5351 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELJO010000230 DAELJO010000000
VERSION     DAELJO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.232"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            373..1899
                     /locus_tag="KPB88_003455"
     CDS             373..1899
                     /locus_tag="KPB88_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0055692.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKKETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKVAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTVAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1927..2484
                     /locus_tag="KPB88_003456"
     CDS             1927..2484
                     /locus_tag="KPB88_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055693.1"
                     /translation="MNKLLKPITIILYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            3006..4049
                     /locus_tag="KPB88_003457"
     CDS             3006..4049
                     /locus_tag="KPB88_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055694.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4160..5302
                     /locus_tag="KPB88_003458"
     CDS             4160..5302
                     /locus_tag="KPB88_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0055695.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2267 a          438 c          941 g         1705 t
ORIGIN      
        1 aattaattta tataaaataa atttttaagt aaatattttt atagagagat tgttatatta
       61 catatcagaa aaatattaaa ctaactaaat caataaaaaa gttggtggct aacagtttat
      121 aattgttagc acaacatcat cttatgtatt gaaatctaaa atatcctcat ttttacttag
      181 cataaatact attaattaag aaaataatat agaaattata aatatatata agtttagtct
      241 ggtgtataaa acaagaaaac taagtgattt ttaatttagc tttttattaa gcttattttt
      301 tatactattt ataataaata aactccatga tgggaaaact attactattt aatatgtaaa
      361 ggagtatttt aaatgaatga atcatattgg tttttaaact caagtccaaa agaatataac
      421 aaattaggtg aaaatataaa aacagattgt cttatagtag gtggtggaat aactggattg
      481 acaactgcat atttattagc aaaagaaggt aaaaaagtag tgcttgttga ggctgataaa
      541 ataggatatg gaacttctgg aaggaatact ggtaaggtaa cttgtcagca tgatatattt
      601 tactctaaga tagagaaaaa atatggatta gataaagcaa aatcatatta taatgcaaat
      661 aatgaagctt taaatttagt tgaacaaata atagaagaaa acaatataaa atgtgatttt
      721 aaaaaggaaa cttcttttgt atttacagaa aaagaggata ctataaagaa tattaaagat
      781 gaatatcgta catgtaaaaa aataggaata aattgtgagt atcatgaaac tatagaaaat
      841 attccattag atataaaggg ggctattagt tttactaatc aaggtcaatt taacccaaag
      901 aagtatattg atggattggc aaaagtagca gtaaatttag ggctaaaaat atatgaaaat
      961 acaccagtag tagaccttga aaaaggaaaa atatgtaggg ttaaaacaag agaagataat
     1021 ataatagaag ctgagaatgt tataatttca tctcatagtc catggtatga tggattgaat
     1081 ttttattttg caaaagaata tgcagagcgt gcttatttaa tggttgcagt tttggaaaat
     1141 aaattagcag atggtatgtt tataagtata gatgacccat caataacttt tagacaatat
     1201 aatgatggaa gtgaaaattt actgattttt ggaggaggag accataaagt tggtcaggga
     1261 ggaactgaga aagaaatatt tgatgattta gagcattatg gtaaagaagt ctttaaggta
     1321 aaagatttta aggggaaatg gtctgctcaa gataatatga gttttgacaa tgttccttat
     1381 attggttata tcaacaaaag agaagacaat atttatgtag ctacagggtt ctctaaatgg
     1441 ggaattacta atggaactgt tgctggaata ataataaaag atttgataat aaataataat
     1501 tctgattata aagatacctt taatccatcc agattaggta gctacttttc aaaagatttt
     1561 ataaaggaaa atgcaaacgt agcaataaat tatgtaagtg gaaaattaaa aataggtagt
     1621 ggagatatgc ctaaaaataa tggagaagga aagattgtta atattgatgg aaaaagatat
     1681 ggtgtatata aggatgataa tggagatttt tatattgtag acacgacatg tactcattta
     1741 ggatgtgaac ttaattttaa cagtgaagaa aaaacatggg actgtccatg tcatggttct
     1801 agatttgact ataagggaaa tatacttgaa ggaccagctc ttaagccatt aaaactttat
     1861 ggacatggtg ataatgatgt taatcctaag ttattataaa attattttta ggaaggtaaa
     1921 gtgtatatga ataaactttt aaagccaata actataatat tgtatatagt ttatatttta
     1981 tctgccgtat tttttgtagc aaacaaagaa tatggaagtc taggtctagt agcaattagt
     2041 ttagttggta ctttcatttt gagttttata aataagcaaa ctgaaagatt attgagtaaa
     2101 gaattatata taatgcttgc tttatttata atgttttcat cattgttagg tacttgtttt
     2161 aatttttatg atataaatta ttatgatgat tttttgcatt tatggtcagg tataatatct
     2221 tgtattgtgg ctttttctat aattaaatac ttttatacac aaagagagat aagtaaaatg
     2281 agtaaagtgt ttttagttat atttttattt atgtttagta tgggagtagc gagtttatgg
     2341 gaaataggtg aatttttaat ggatactttt gttgggacta ctacacaggc cggagggtta
     2401 gaagatacta tgattgatat ggtagatgct ttaataggca caattataac tattcctttt
     2461 atagtaaaaa aatctaatat atagttttaa attttttata ttaattataa tggatttaat
     2521 taatcatgtt gagtttcaag gtctatatga ttaattaaat ccatttttgt tatttataat
     2581 tattattgaa tatattttta catataagat ataaggtgat agatagaaat tgttggatta
     2641 tgtaaataat taatatataa taccattatg ggcaataaat acaaatcaaa tgataatata
     2701 gtctatttat gtaaatgtta tattgtatcc taagtataat agtaagtttt ttgatagtgg
     2761 tgtagaaagt aaaaatctac tagcctaaat tgatgtatgg attagtttag gagcagtaat
     2821 tgcatatctt aagtttgatg tcatgttggg ctactagaaa taggtcatct ttttcacttg
     2881 aatatctttt ctttaaacat ggggagtatc atatcaaaaa catatgaaaa ttaagtgatt
     2941 tataagaaca gtaatatttt atatgatagt atagaatagt aataaagtat taggaggaac
     3001 attgagtgtt aaaaaagtgg tttggtattg tgaaaaaaag acaaaaaagt gagtcagtaa
     3061 aagaagaagg tgaagtaata ttaaaaaacg aaaaaatatt atctgaagaa aagttgatag
     3121 atgaagaagg agttgtagtt aatattgata atgaaatatt aactaaagta gaagtagtaa
     3181 atgatgacaa tgaaatagaa gaaaaaataa tagaagaaga ttggttaatt agtgaaaata
     3241 ccataaaatt agatgataaa gaagcaatta ttaatgataa aaacatagaa ttatgtaaaa
     3301 aagaagttca agttgaaagt gaaaaaatag gtttaaataa gtttgaagga cttgaccaaa
     3361 atgaaaatta taatctagaa aaaaatgtta ttgaagaaaa agaagtaagt gaatgtttga
     3421 cagaagaaga tttagagtac ataaaagaaa ttaaaataaa aagaggaaaa agtataaaag
     3481 ctataaactt gtatactaaa gaagagtggg tttttgacac tcatatacag tgtagtaaaa
     3541 aactcaaagt tccattaggg tacataagag aaaatttaaa atatggatat atggattact
     3601 ttggagatgc aataaattat ttaagtgaag tattaaatat agatgaatac tgcaaaagtg
     3661 aatggagcta tctcgataat agcaaatctc catctgagat atttaatatt ctaaacaata
     3721 aaatatttag tataaggctt tctaatgaaa aaagaaatga aattttgaca aatgataaaa
     3781 ttgaagcatt aaaaatgaat tatagatttg aatgtattga tgaagaatat gatgaatatt
     3841 ttaaaaaata taagtctata atcaaaagag gtggaaagaa aaaagttgaa ttagtaaata
     3901 aaaaaggtga cattttagaa atatttaagt ctttagaaga atgtgccatt tatttgcaaa
     3961 aggaaaagaa tgaagttata caaatgttaa aatatggaga tacaaaagta ggaagaaact
     4021 ttataaggta tagtttgaga agtatttaat ttcattttaa attcataaga gtttcatttc
     4081 tgattcacac atttaatatt attttaatgt aaaataataa aggtgaagta ttgcacaatt
     4141 aaaattaagg agggtattaa tgtctgaaga aaaaaatcca aaagaaaata cattagatga
     4201 atctaaaaat tcagataatg taaattctaa aaaagatgat gtgtcagatg aaaaaaatac
     4261 tgataacaaa gttaaaaaaa gggaaataaa gctaaacaac aaaacactta aagctattgt
     4321 agcaattgtt gcagtaggag ctatatgttt aggtggaggc tatgtttttg gcaaagaagc
     4381 tggaagaaaa ttgcctgcta cttcaaaaca ctataatagc agtgaggtag tagctactat
     4441 tggggataaa aaaataacag gtgaaatgtt aagaaagaga atggagccat tattttattt
     4501 aaatgcaaaa actactatga ctgatgacga aatacgttca tatgaatcaa atatgattga
     4561 aactatgatt acatctgata tattatcctc tgaagcagca aaagagaaaa taagtataac
     4621 taatgaccaa ctagatgctc agtataataa tttaatgagt agtatagaat cagcaatggg
     4681 aatgactaaa gaagagtttt taaaacagtt tgatttgact gaaaaatata taaaagatga
     4741 tatgaaaaaa gagctagtag catcaaaata tttaaatgaa aaatcaaaag ttactgataa
     4801 agaagcagag aactattaca ataaaaacaa gagcaattat ttacaagtta gagcttcaca
     4861 tattttaata aaaactgttg atgataaagg taagcaagtt agtagtagca aaaaagctga
     4921 acttaagaaa gaagctgaag aaatacttaa aaaagcccaa gctggggaag attttgctac
     4981 tctagctaaa aaatactctg aagatagtag tgcagaatca ggtggagatt taggtttctt
     5041 tggtaaaggt cagatggtgg aatcttttga aaaagcagcc tttgcattga agaagggaga
     5101 ggtatctaac aaacttgtag aaagtgatta tggatatcat ataattaaga aaactgatga
     5161 aaaatatcag ccatttgaag aggtaaaatc agatttagta agcagtttaa catcagaaaa
     5221 acaaaaccta cttatacaaa atctaaaaga aaaatataat gttaaaatta cagataaata
     5281 tcaaatgaaa gctaaagaat agataaagtc aatttattaa aaaaaagaaa aattaactgt
     5341 atggataaaa g
//
LOCUS       DAELJO010000231         5348 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELJO010000231 DAELJO010000000
VERSION     DAELJO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.233"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            98..934
                     /locus_tag="KPB88_003459"
     CDS             98..934
                     /locus_tag="KPB88_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0055696.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1287..2864
                     /locus_tag="KPB88_003460"
     CDS             1287..2864
                     /locus_tag="KPB88_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0055697.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPVEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTTVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2857..4041
                     /locus_tag="KPB88_003461"
     CDS             2857..4041
                     /locus_tag="KPB88_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0055698.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLKHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEESEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4059..4658
                     /locus_tag="KPB88_003462"
     CDS             4059..4658
                     /locus_tag="KPB88_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0055699.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4682..5152
                     /locus_tag="KPB88_003463"
     CDS             4682..5152
                     /locus_tag="KPB88_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0055700.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2005 a          562 c         1020 g         1761 t
ORIGIN      
        1 aaatatatac gaaagtaaaa ttttaaatat tgacattaaa ttaaatatac tatactatgt
       61 aagtgtgatg agagaatttt tttagagggg atttatagtg aatataaaaa agatatttaa
      121 taacaatgta gtagtaagct ccctagaaga tggcacagaa ataattgtca ctggtgctgg
      181 agtaggattt aagaaaaaag taggcgattt gatagatgaa aatttgattt ctaagaaata
      241 ttttgttcaa gacgatcaaa gggacaaata caatcaaata cttaataaga cctctattga
      301 atactttaaa atttcagaag aaattataga aaaggctaat gaagttttaa atactcaagt
      361 gaatgattca ataatattag ccttaactag tcacatagaa tttgctgtcc aaagagaaaa
      421 acaaggtata aaattaccaa acttaatatt gaatgagaca aaacaacttt atagggaaga
      481 atttgaattt ggattatggg caattgatga aattgaaaaa aggataggaa taaaattgcc
      541 tgaagatgaa gcaggatata ttgctattca cataatcaat ggtttggaaa attctcaaaa
      601 agatgaaggt attaatatac tagagttttc aaaacaagtt attcagataa ttgaagaagt
      661 acatggattt aaattggatg taaattcatt aaattatact agacttataa ctcacttaaa
      721 gttttttgtt caaaggattc tccgaaaaga aacttacaat gatagtgata ttgaagatat
      781 gtataagtta gtgaataaaa attacaataa acctaaagtg tgtactgaag aaattgcagc
      841 attggtatta gacagatttg aatataagat tagtaaagaa gaagaattat atttaatgat
      901 tcacataaat aaaataacaa acagtggttg ttaggattgt aactgataaa gcaggcaaaa
      961 cctaagtcat tgagcttaac tattagatta agttttttga tttaggtttt tttattagat
     1021 acaatcaaaa tacagacagt gacttttagg attgtaactg gttaagcagg caaaacctaa
     1081 atcattgaat ttaactatta ggttaagttt tttgatttag gtttttttat tagatacaat
     1141 caaatacaga cagtgacttt taggattgta actggttgag caggcaaaac ctaaatcatt
     1201 aaacttaatt attaaattaa gttttttgat ttaggttttt ctataaattt gattatgttt
     1261 ttagataaaa ataggagggg aaaattatga aaaaaaataa gttaggttta tggaacttct
     1321 tccaaatgtt aggaaaaacg tttatgttcc caatagcact acttagtgtt tctgggatga
     1381 tgctaggact tggggcaggt tttacagacc ctaagatgat tgaaatggtt ccatttttag
     1441 ggaatgaata tgttaactta gttttaaact tcatgttaac aataggatta tttgcattta
     1501 acaacttggg agcattattt gcaatggcaa ttccactagg attattaaaa aaagaaaaag
     1561 agtttggtgc tttttcagga ttagtaggat ttatagcaat gcacataggt acaaattttt
     1621 atttaacaag agctgatttg ttagtaccag tagaacagat gtcaactaat ggtcaagcta
     1681 tgattatggg tatacaaaca tataatacaa gtgttttagg tggtattgta gcaggattaa
     1741 ttgtttatgc aatatatgat aaagttagta atttaaagat accagaatca ttaggattct
     1801 acagtggacc aagattagtt ccaattgtta gtttaatagt tatgagtata gttggattat
     1861 taatacctat tgtttggcca ccatttttca atgcattcca aagtttagga agatggattt
     1921 cttcagcagg accattagga tatttttcat atgcagtggc agaaagagtt acaattccat
     1981 ttggactaaa tcatcttgta acatccgtat tccgttttac accaataggt ggaactactg
     2041 ttattgatgg agaaacgtac tttggtacat tgaacatgtt tatggcatat gtagaaaata
     2101 atcagattat acctcttgat ttagctggta aaatggaaca aggtaaattg atgattcaat
     2161 atggattagc tggtgctgca ttggctatct accgtacagc aaaaccagaa aatagaaagg
     2221 cacttaaagg acttttaata tcaggagtgt tgacagttat tataggtggt ataagtgaac
     2281 caatagagtt tctattctta ttcatatcac cagcattatt tgctttccat acatttatga
     2341 atggtctagc aaacatggta cttccatact taggcgtgtt gatgggattc acaggagatt
     2401 taattgcatt tataagtttt ggtgtgttaa gaggaacagc tacaggatgg ccaatagcag
     2461 tattagtagc agttttatat tttgcaatat actattttgt atttaagtgg gcaattatta
     2521 agtttaatat aaagacccca ggacgtgagg ataaagaaat aaaagtatct gaaggaaatg
     2581 gttcagattt caaaaatttg tctacatata aaggacaaca aatgatatct gctttaggtg
     2641 gtcaatctaa tatattatca ctagataatt gtgtaactag attacgttta aaattacaag
     2701 atgtatcatt aataaatgag gatgcaatta aagaggctgg tggtatagca gttgttaaat
     2761 tagatgagca tacaatacaa gtaattatag gaactcaagt gtattcttta agaaagcaga
     2821 tggataaagc tatggaaggt catttggagg catgtaatga ttaattttga taaaaaagta
     2881 gaccgattgg gtacatattg tacccagtgg gactatgtag aagatagatt tggtaagaag
     2941 gatttgcttc cttttacaat ttcagatatg gatttagagt ttccatttga aataacagag
     3001 gtgttgcagg aaaggttaaa gcatagagtt ttgggataca gtagatggaa acatgatgat
     3061 tttagaaatt cagttacgaa ttggtatatt aaaagattta attgtagcgt tgatagtaac
     3121 tggatatatt atagtccaag tgtaatgtat tcaattagca aacttattga gatgttaagt
     3181 aatgaaggag atggaatcct tataaatact cctgcataca atgcttttta tgatgtgata
     3241 ataaataaca atcgtaatat tataaagtca catcttgtaa ataggaatgg attctattca
     3301 attgatttta atgattttga gaaaaagtgt aaaaaatcta agatatttat tttatgtaat
     3361 cctcacaatc caactggtag agtatggagt gagttagaac ttgttaagat gataggaatt
     3421 tgtaagaata atgatgtcaa gataatatca gatgatatac acatggatat tgtatatgac
     3481 aatagaatga cacctatctt aaatgttgca aaggactatt tagattctgt attcatttgt
     3541 acatcagcgt ctaaatcttt caatatacca gcattgactg ggtcatatgt gattatacca
     3601 aatgaaggta ttagaaataa atttgaaaat atcactagat accgtgattt tgtaaattct
     3661 ccagcaattc tagcaatatt agctacaatg gtctgttatg atgactgcga atattggtta
     3721 gatgaactat taaaatacct taaagagaat cttaaatata cgattgatta tataaaagat
     3781 aatttagaac cattgaaatt ggtcatgcca gaaggctgtt actttgcttg gatagatttt
     3841 tccgaactta aaataagtgg tgagaaattc caaaaattat tgatggatat tggagaagtt
     3901 gctattatgt cggggtctgt atatggtgaa gaaagtgagt tttatttaag gttaaatgtg
     3961 gcatgttcaa gagaaaaact tgaagatggt ttaaatagga ttaaaaaaac tatggattat
     4021 attgcaaaaa gaattttata gtaggaaggg agatagttat gggatattta aaaacaatgg
     4081 ttgatgttat gactactgaa atgaatgcca taagatgttt aatagatgaa gcaggaatag
     4141 aatatgaaag tatagttagt gaaattgcaa attgtgaagg caaagtaatt tttatgggtg
     4201 taggaaaatc tgcacatata ggtaagaaat tggcagctac atttgctagt actggtacac
     4261 ctagcttttt tgtacatgca acagaggcag tgcatggaga tttaggaatg attgaaagta
     4321 aggatattac aattttaata tcaaatagtg gaaattctat ggaagtggta aactgtataa
     4381 aatatattaa agcaataggt tctaagacaa ttgcatttac ttcaaacaga aattcagtac
     4441 tggcaaaaga gtgtgattat gctttaatat atcctgcaaa ggatgaagca gaccatttaa
     4501 atctagcacc aacaacatca tctacaatta ctttggtttt aggagattct attgcatgtg
     4561 cattatctaa gagtagtaat tttggttctt cagattttta taagtaccat ccaggaggaa
     4621 gtttaggtga gaagttaaaa acagctaaca atggataaaa gcaaaaagga gagtagtttt
     4681 tatgttcaaa atgtttaaaa aaaaatcaat aaaatcaccc atatcaggaa aagtaataga
     4741 gttatctaaa atacaagatg gtgtattttc tcaaaaaatt atgggagagg gtgtagctat
     4801 agattctaca ggagacattg tatatgctcc aatcaatgga aagatagtag tagtagcaga
     4861 gactaaacat gcatttgtaa tagaattaga aaatgggatg gaacttctta ttcatgttgg
     4921 tttagatact gtaaatttaa aaggcgaagg ttttgaggta ttggtgagta tgggagaaca
     4981 agtgaaggaa ggaacaccaa tgttgaaaat taaccgtcct ttgattgaat caaagggtat
     5041 atcacttatt actccagtta caataactaa tcattcagag tacaatatga atacatgtaa
     5101 tgtaggcaat agtgttgaag gtgggaaaga tactgtaatt gagtttaaat aaagaactaa
     5161 aatacaaggt taatatttat aaatacctat aaacttatgt tttataagat tgattgtata
     5221 gatttgttgt ataaaaaaga actctttaat tataaattaa ggagttcttt tttattttgt
     5281 aaatattgtt tagatttata tagaaaaaca cttagtaata aatagaataa ataagtattt
     5341 atatagct
//
LOCUS       DAELJO010000232         5347 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELJO010000232 DAELJO010000000
VERSION     DAELJO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.234"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            83..283
                     /locus_tag="KPB88_003464"
     CDS             83..283
                     /locus_tag="KPB88_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG0055701.1"
                     /translation="MDDISRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            337..1500
                     /locus_tag="KPB88_003465"
     CDS             337..1500
                     /locus_tag="KPB88_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0055702.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(1750..2172)
                     /locus_tag="KPB88_003466"
     CDS             complement(1750..2172)
                     /locus_tag="KPB88_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG0055703.1"
                     /translation="MENKNSKCVMVIDENLPMGVISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(2193..3029)
                     /locus_tag="KPB88_003467"
     CDS             complement(2193..3029)
                     /locus_tag="KPB88_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055704.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3327..3923
                     /locus_tag="KPB88_003468"
     CDS             3327..3923
                     /locus_tag="KPB88_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG0055705.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            3953..4672
                     /locus_tag="KPB88_003469"
     CDS             3953..4672
                     /locus_tag="KPB88_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG0055706.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            4674..5213
                     /gene="scpB"
                     /locus_tag="KPB88_003470"
     CDS             4674..5213
                     /gene="scpB"
                     /locus_tag="KPB88_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG0055707.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT         2069 a          605 c          836 g         1837 t
ORIGIN      
        1 atgttacata aaataccaat tataacaatc ttttattagt aaaaactact tatatataaa
       61 ttttactgat aggaggattc ttatggatga tatttcaaga caaaatgcta taaaagcttt
      121 aaaacaaaca aaaatggaaa tagcaggaga gtatggaatg aattatgaag atgcttttga
      181 aataatagaa aatgcgtcta ataaaggtgt tttagaaggt tactttaaaa agcttgaaaa
      241 gaaaaaaaat ttaggtcaag gtatatctag gcatttagaa taaaaaaagt taaatgtgag
      301 aaaataagtt taaataaact ttaagaggtg acatcgatga aaaaattagc tagtactgct
      361 ttggctatat taattgcgct tactccttta agcttttctt ttgcaaataa taaggaaaat
      421 gctgatgcca accaactaaa tatatcttca aaatcagcaa ttttgatgga tgttggtagt
      481 ggacaaattt tatatgaaaa ggatgctcat aaaaagcttc ctcctgctag tgtaactaag
      541 gtaatgacta tgctacttat agtagaagct ttggattcag gcaagattaa gcttgatgat
      601 gaagttcaag taagtgaaac tgcatctagt atgggtggta gtcaaatatt cctagagcca
      661 ggagaaactc aaaaggttga tacattatta aaaggtatag ctgttgcttc tgcaaatgat
      721 gcttgtgttg ctatggctga acacttagct ggaagtgttg aaggatttgt tgatagaatg
      781 aatgctaaag caaaagagtt aaatatgaat gatactcatt ttgcaaatac aaatggactt
      841 ccagttgcaa accactatac atctgctcat gatatagctt taatgtctag agaactatta
      901 aaacacgaaa tgataagtaa atacttaact acatggatgg ataaggttgt agtagggaag
      961 aaacaagtta cagtaggtct tgcaaatact aataaactta taaagcatta tcaaggagca
     1021 actggtgtta aaactggttt tactcaagaa gcaaaatact gtttatcagc atctgcaaag
     1081 agaggtaata ctcatctagt tgctgcaact ttaggagcag agacttctcc agaaagattt
     1141 aatgatgcct ctagcctttt aaactatgga tttgctaatt atgaaagtgt taagttatgt
     1201 agtaaaggtg ataatatagc aacacttaca ttggataagg ctgatgaaaa caaagttaaa
     1261 ttggttgcta aagaagattt aaatgctctt attaaaaaag gaagttctaa agaatttgag
     1321 aagaaaattg aaatagtaaa aaatcctaag atgccaatta aaaagggaac tgttttagga
     1381 aaaataaaga tatgtaaaga taaaaaagta ataggcgaag tggaattgat aaatactaag
     1441 gatataaata aggcaagtta tctacaaatg cttcaaagaa taattgataa tatgatataa
     1501 ataattatca gatgaaatat aattataata cacaaagata tgattataat acacataaaa
     1561 aataccctta tgtaattatt tgccataggg gtatttttat ttaccatata gaagtatttt
     1621 ataatcttat gatttttatt taccatatag aagtatttta taatcttatg atttttatag
     1681 aaatatagaa aagtttatgt atcttaaaat atcatttgat ttaatatcat atatagggtt
     1741 gtttaacaat tatctaagta aaggcatact accagtaagt ttattcactt tctttttatt
     1801 cccataaatt gctatcccaa aatactgtaa ctcatcttct ggaacactag caatcttttg
     1861 tgtaaattct tcataaagat tgcaactttg tgctacatct gaaaaatcta caacagttaa
     1921 atcgttaaat tccaaagtat ataatttttt acgtaaatct tttattattt ctttgtttcc
     1981 ttttaaaata ggtacaggaa tgtcaataat tcctaagtgg ctattacctg ttttgtcaat
     2041 tacatctggg ccaacagttt ctggggcatg ttttcctagt gtaattccca ttatagctgc
     2101 tgtattggat ataactccca ttggtaaatt ttcgtctatt accatgacac attttgaatt
     2161 tttattttcc atttttattt ctccttttta ccttatttaa agttgtttga atcatcattt
     2221 ataaatatat tttgatattg ctttggtgta agaccaataa aattcttaaa aaagtttgta
     2281 aagtgacttt ggtctacaaa tcctgtttgt atcgcagcat ctataggttc tacgcctttt
     2341 tctaaaagct ttttagcttt gtttacccgt atattttcta ggtatctata tggtgtaata
     2401 ccttttaatt ttgtaaaatt acgaagtaga gagtacttat tcatacctgc tatagtgctt
     2461 aattcgtcaa gtattatatg ttcagcataa ttattttcta gataatcaca tactgcttga
     2521 atttctatat ttgtattttc caagttagat tgcaaatttg gctctgtatg ttcttcaatt
     2581 aattgttcaa ttaggaagaa aaaaagctct tcctttttaa aatccaattc ttcttccatg
     2641 attatatagt gtaattcttg tagtagaggt actagctcac tgcgaaatat aacaggttga
     2701 ttaaattttg gtaaataatt tttacctgta atttcaaaga cggttttttt cattatttct
     2761 ggcttaatat taatacatct gtaatcaagt actttatcat ctatttgttc acaagtatga
     2821 ctatcaaaag ggttaaaaag tagcaaatct ccagtacttg tggtatactc tttattttta
     2881 caagataaat atctttgacc tttttctata aaaccaatta cataatgttc atgaaaatgg
     2941 tttgggaatt tttgcattat gcctttaaat ctatatgctt caatttttaa ttccctatca
     3001 aagcatactg tgcgttcttc ttgtctcact taggtaacct cctttcatgt tatacaaagt
     3061 gtaacacata tttttttata cttcttgtat aatattgact aagagacaat aatgtcaatt
     3121 catgtcaata taatattaca cttacagtag gtgtaattat tatattttca caatgaatat
     3181 gatataataa taaaagttat aaaataaaaa gcaaatttta attttatgcc tagaagttaa
     3241 aattattata atgtaaaata attttgatta taagctattt aattgttata ataatattaa
     3301 aaatttaatt taggagatgt acattaatgt taagtatgaa tataggagaa atagtagcaa
     3361 gtttagttgg tatagctatg gcaatatcaa tacatgagtt tggccatgct tattcagctc
     3421 acttattggg agatgataca gctaaagctt atggtagaat gacattaaat cctgtaagac
     3481 atgttgaccc aataggtctt ttggctatgt ttatccttaa gatagggtgg gcaaagccag
     3541 taccagtaaa tccaaataac tttaaaaact ataagatagg aaacctgata gtttcacttg
     3601 caggcgtttt ttgtaatgtt ttgactgcta tactgtgtgt aattatcaat aagtatgtgc
     3661 atatgtatgc tataaataca atagctggat atgtgctttt atatagtatg ggatttgcag
     3721 cttttaactt acttccgata cctccacttg atggatgggg agttatatct acatttgtac
     3781 catataaatg gaatgaatat ttgtataaat ttgaaagtat gagttatata attttattaa
     3841 ttgctttatt tactggtatg tatagaataa tattaacacc aatatatgca gtgttgctaa
     3901 aaatagtata tttatttgtt taatataata aacagattaa agagggattg atatgaagta
     3961 taatatacaa ttacaagtct atgagggacc attagattta ctttatgatt taataacaaa
     4021 gcataaaata gatataaagg atatatccat aatagatata actaagcagt atctaaatta
     4081 tttgaaaatg ctagataaaa tggatttaga gataactagt gaatttataa ctatggcatc
     4141 aaaactttta gagataaagt ctaaatatct gctttataag caaaaagatg aagaagaaga
     4201 cccaagaata gaacttatgg aaaagttaga agaatataga aagtttaagg tagcatcaca
     4261 agatataaaa gaaaatataa cttatgttaa tgagagattt tatagaaata aagaagagat
     4321 aattattgat gacaatgtgg atttagaaga tatatcaatt gaagcaataa aaaatatact
     4381 tccatacata tttaaagtta aaacatcaca gatagaaaat gctaatgatg agaaactaga
     4441 taaaatagta aggaagaaaa taatatctgt agaagaaaaa atattatata taagagatat
     4501 tataaaagat aagattgaag ttacttttac aaatattata aaatcatatg aaaatgatga
     4561 aataatagct acttttttat ctatattaga gcttataaaa gaaaaagaaa tagtcgtagt
     4621 acaagacata ttttttgatg atattctaat aagaaaaagt tcggagtgtt aaaatgaaaa
     4681 gagaagatat aaagtatatt atagaatctg ttatgtttgc atatggggag ccaataagta
     4741 taaaagaatt aaactatata ataaataaag aactttcatc aaaagaaata gagattatgt
     4801 taaatttact tattgaggag tacagagaac aaaatagagg tattcaaata atcaagttag
     4861 agaataagta ccaaatgtgt acaaataaag actatgcaga atatataaag aagattattg
     4921 aaccaaagaa aaagaaaagt ttgagccaag ctacattaga aactcttact ataattgcct
     4981 acaaacaacc aataacaaag gtagaaatag aggatataag aggagtaaaa tgtgataagg
     5041 tattacaaac tttgtttgaa aatgaactta taagagaagc tggtagattg aataagatag
     5101 ggaaaccaat aatttataaa acaacagatg aatttttaaa gttgttgaat atagaaagtc
     5161 ttgaagaatt gccacctata gaaaattatc aagaagtggc aacaaatgaa taaaaatata
     5221 aaatcacaat aatttagtat gtaattttaa attttaaaag atttaaaatg ctctaagttg
     5281 ttgtgttttt ttgtataaat ataaaattat aattctctta aaataaatat ggataattaa
     5341 gtataaa
//
LOCUS       DAELJO010000233         5276 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELJO010000233 DAELJO010000000
VERSION     DAELJO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.235"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(25..927)
                     /locus_tag="KPB88_003471"
     CDS             complement(25..927)
                     /locus_tag="KPB88_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0055708.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(977..1438)
                     /locus_tag="KPB88_003472"
     CDS             complement(977..1438)
                     /locus_tag="KPB88_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG0055709.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1490..1945)
                     /gene="def"
                     /locus_tag="KPB88_003473"
     CDS             complement(1490..1945)
                     /gene="def"
                     /locus_tag="KPB88_003473"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0055710.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1917..2180)
                     /locus_tag="KPB88_003474"
     CDS             complement(1917..2180)
                     /locus_tag="KPB88_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055711.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2277..2993)
                     /locus_tag="KPB88_003475"
     CDS             complement(2277..2993)
                     /locus_tag="KPB88_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055712.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(2990..3673)
                     /locus_tag="KPB88_003476"
     CDS             complement(2990..3673)
                     /locus_tag="KPB88_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055713.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3670..4515)
                     /locus_tag="KPB88_003477"
     CDS             complement(3670..4515)
                     /locus_tag="KPB88_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055714.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTVIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4629..5219)
                     /locus_tag="KPB88_003478"
     CDS             complement(4629..5219)
                     /locus_tag="KPB88_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055715.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         1852 a          848 c          506 g         2070 t
ORIGIN      
        1 taaaaaaaat ttttttatat aaaactaatc aaaaaaagat atctgtacat ctcctgagtt
       61 ttttaacttt tccttcatta ttttagagta actgcttgat agagaatact tatatctaag
      121 ttcattaacc attttataca attgattttt atactcccta ttaagtgaac ctgcactata
      181 tagtattgat aatttatcaa ataattcagg aaactctttt ttaacaaagt caaaaaaaac
      241 acctctagtt atacccctta agtataaagt cccaggtaaa acataatgta cattacattc
      301 ttttgcatgt ctaaatagcg aatttatatt atcaaaatca tctgtaatat atggtataat
      361 tggcataata tgtagaccaa ctgatgcgtt agtttttcta aattctttta acattttaaa
      421 tcgtctcatt gaatccacac catttggctc aataagcctt tgtgtatctt catttactgt
      481 ggtaattgta gatgctacat tgatatatgt aatcctagat agcttatcaa ttaaatcgta
      541 atctcttaaa attaaatctg actttgtaga aataatagct ggtgtcttgt attttattag
      601 caggtttaat atttctggca taattttata gtcagcttct attggttgat aactatctgt
      661 aactcctcct atattgatta cttctttctt ccatttactg cttttaagtt gtctctcaag
      721 tttttctact atatttgttt ttacataaat ctcttcaaaa taattattgg agttaatgta
      781 cttatgagag taaatagcat aacaatattt acatccatgc tcacatccac gataaatatt
      841 taaatcccat ttatatggca tactcctttt taatttatta caagctattt cacaatttat
      901 tggaatatat tttattttat tatacaaata atcacctctt tatatacaat aatactataa
      961 tgttaatcta tagtaattaa aacatgatat ttccagacca caattcataa tctttactat
     1021 atttacaaag taatatattc cattcattta ttggtattcc tccacatgtt gctttttctt
     1081 ctaaattata taagcattgc tttaaataca ttttattatg ccaatcatca tataaatttt
     1141 ctaaatttaa actatgttca ttagatatat catttgcaaa atttctattt ttccatttta
     1201 atatacttgt atatttaagt tcattaggtt tataatttct ttttaacatt tcatcatgta
     1261 caatttttgt ataaaattta aattcatcta tactataatt caatacttta ttcactaata
     1321 gatggtttgg agtacctttt ttatctatag aaccttttat agcaagtagc tctctccatt
     1381 gacttactaa ttgattttta ggtaatacat caattaaatc cttatgccat aatctcataa
     1441 tttaactcct tactataaaa tattttataa attcaataac caatctatct tacaatttta
     1501 catgttcaat aattttatct gtaaaaacta taccatttaa atggtctatc tcatgacaaa
     1561 gacattttgc cataaaacct gaacctttaa tttctatttt ctcaccgtat tcgtttagag
     1621 cttgcactgt aacttttttt ggtcttttta atttacccca tacttcagga aaacttaaac
     1681 atccttcaac aacaatttgt tccccttctt gtttaataat ttcaggatta acaagcttaa
     1741 taagtccttc tcctaaatct ataacaacta atcgctttaa tactcctact tggcatgcag
     1801 ctaatcctcc tccatttgga gtattgtaca tggtctctgc catgtcattt aatatttctc
     1861 ttatcttatt atctacgttt tctacatact tactcttttt tcttaaaatt tcatcatcaa
     1921 atgttcttat ctttcttata gccataatat tctcccctac ttatctgtgc tcttgatttt
     1981 ctttcacttt cttcaaataa aaatacactt tcaattggtt cattaaaaat tcttgaaata
     2041 tcatatgcca accatattga aggttcattt ttaccagttt ctatagcatt tatagcctgt
     2101 ctagatgttc caactaatct tcctagttgt tcttgtgtca atcctaattt ttctcttaat
     2161 atctttattt tatttttcat atttgctcca ttcatataag gttaacttta catcttaaat
     2221 gtaacatttt tcaacattac tatcaagtca accttacatt tatatattaa attttcttat
     2281 ttaactattt ttttactaaa ctttttaaat aataataaaa tccatattaa tgatataaca
     2341 attgaaataa acatatagac ataattatct tccttaaatg ccttcgatat ccaaaaagtt
     2401 ggtaagaaga acagaatata ttgtgcttta tttaaaataa aaaatggtgc taaaataccc
     2461 atcataaaaa cacctgagaa tttcgtaatt accattcctt ctagtttatt tgatgataaa
     2521 gaaatcacta atattgatat aataattcct tgtaataaaa ctaataaaga aattgataag
     2581 tttaacaaga gagaaatttt agttagagaa aaaaagataa gtagaatcat agtaataata
     2641 aatgctaaga tacctggtat tccaaatcgt gaaataagat aaccagtttt accaagcgga
     2701 gttactatta aatatcttga aatgctgtca tcaatttctc ccaatatgac cattgtagaa
     2761 gcaaaaaaga acataaaagg tgtaataaaa gctaaaagta aatcaaacat caagaaatac
     2821 ggctctaaat ttaattgata ataaaacttg ttaagtgata tattctgtat taatggtatt
     2881 ccaaatttta taaatactcc acataatatt ggagaaacac atactataaa aagcatagca
     2941 tctctcttaa tttgttcaaa ctcttgttta aacgcattta taatctttat catagtttta
     3001 ctccacctac ctttttccaa ctgctacaaa taaaataata agttataaaa taaattatta
     3061 ttataattga tattaaaatt aaaatattaa tcattataaa atttttatct ccactaataa
     3121 gagaaatgca tatattaaaa gggtacaaat ttgcatatga ttttgtgtca agaagtacat
     3181 tcagtattgg tggaataaag catataatct caataggaat acttaaaaca ataaactgat
     3241 tcaaactaga tgctttactt gcaattatca atccaagcaa agaaaatata atagaagaaa
     3301 aaaaggtacc tatagttgat attataatat tatctaaatt taatgtaata gcaataaaca
     3361 tagctacaat actagatatt actcctaatg aaattacctt tgataaaata tactcagata
     3421 cttttactgg agatacagct aaggaattta aaaccctttg acttttctca agaagtacaa
     3481 tagctcccat aaaaaacaat cccattgctg ctgggtcaga ataaatcata attatagcta
     3541 ttttttctct cataaaagtt ggaaatacgt tgataataca aatatatata atacttaaaa
     3601 aaagatataa aaaataaaaa ccatacttaa attgaaactt tatatctcct aaaataagat
     3661 tttttagcct cattgtaaat tcctccctgt tatctcaata aaaatatcat ttaatgttgg
     3721 ttcactgcta tgtattgata aaagtttatt tttctcaata agcatattca aatttttatc
     3781 attagcagta ttgttcagaa aacattcaga agttttttct ccattatcaa agtaagtata
     3841 tctaacttta atagctcctt ttgacataat aagattatgt ggtgtatcta aagcagatat
     3901 tttaccattt acaatgaacg ctactctgtc acaaagctct gttgcatcta acatgttatg
     3961 agttgttaaa ataatagttt tacctttaga tttttctgat aaaattatat ctttcataac
     4021 cttgctattt gatgggtcaa gaccacttgt tggttcatct aaaaataaaa tatctggatt
     4081 atgtaataaa gcttttataa aatttaatcg agatttcata ccttttgaat actcagatac
     4141 ccttttattt gcttcatttt ctagtcctac tgattttaat aactcatcaa tagatagtag
     4201 ttttttagaa tatagagacc caaaatattt taaattttct attacagtca acttttcata
     4261 taaactagga aattcaaaat caactcctat attttcataa aatttatttg agtgtctttt
     4321 actctctact ccatttacaa tgacacttcc tccataatta gttatcattc caattagaat
     4381 cttttgtaat gtagattttc ctgcacctga tggtcctaaa aatcctagta tttccccttt
     4441 tcctactcca aagttaatat tttgtaaaaa atctctatct gtataactaa atgataaatc
     4501 atcaacttta atcattcaat atcacccttt caaatttttg actattttga ctatatggtc
     4561 aataaaatat aatttttttg tttgttaata atttttaata aaagttatta gcaaacaaaa
     4621 tttaaattct attccataag ttgtataact aatccgttta tcattaatct taaagcatct
     4681 tcaaaacaat tacttccaac ctcgcgctta tatagcatgg taagaaaaac tgctctaaat
     4741 gctccactaa agctttctat gtctttattt tttgcatttg gtatgcaaga tataatctct
     4801 ccaatttcaa agtcatcatg tctaaagtgt tcttcaatta tagaatctgg taactttctc
     4861 accaatatct caaattctcc atttatcatg agatttaaaa ttgaagtact atcaaccatc
     4921 ctatagaatt tcataaatat atctgtcaat tgttcacatg taatactttc atctactcta
     4981 tttatctcat ataagatttc tttttgtata gagtcatgta tatctcttaa tacctcaaaa
     5041 aagagcaatt cttttgattg aaaaaataga taaaaagttc cttttggtat ttttacccgt
     5101 tttacaagct catcaacagt agtttttcta ataccatatt gctccataca caatcttgct
     5161 tcttctttaa gtctttttat tatatactct ctttctttat cagaatatga tttaggcatc
     5221 ttttccctcc agtttgacta aaataattta tatagtcaat ataatacttt ttctat
//
LOCUS       DAELJO010000234         5262 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELJO010000234 DAELJO010000000
VERSION     DAELJO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.236"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(361..1767)
                     /locus_tag="KPB88_003479"
     CDS             complement(361..1767)
                     /locus_tag="KPB88_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0055716.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1812..2519)
                     /locus_tag="KPB88_003480"
     CDS             complement(1812..2519)
                     /locus_tag="KPB88_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055717.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2690..3604
                     /locus_tag="KPB88_003481"
     CDS             2690..3604
                     /locus_tag="KPB88_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055718.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3604..4365
                     /locus_tag="KPB88_003482"
     CDS             3604..4365
                     /locus_tag="KPB88_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055719.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4377..5114
                     /locus_tag="KPB88_003483"
     CDS             4377..5114
                     /locus_tag="KPB88_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055720.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1806 a          817 c          688 g         1951 t
ORIGIN      
        1 aggatgtcat tatatttttt cttaaaatta aaaaacacta taccataatt ctattttagt
       61 agaatttttt tgtcaataat aaaattaaat attaactaaa aaataaaaaa aatggaatta
      121 tctaaagaat ttaataacct tacaataatt ccaaaatatt atttctatga aaattttgat
      181 accatgagta actcagccat aagcgcataa agcacaagtt actctttact ttaataaaac
      241 ttgacaagaa aagaatttat ccttattcta ctctaataga gttaatttgt caataaagaa
      301 taaaattatt ttcaaaatat tttcattctt cattcttatc tgttattatt ttataaaata
      361 tcacttaatt agaggtaatt ttagtattgt ttttagtccc tgactatcaa tgctcgtaat
      421 atctaattta cctctatgtg cattaataat gccagatact atcaaaatac ctagaccatg
      481 attttgacct ttggcatatg cctcacttag tccattttgg ctctttaata aactattaat
      541 tctttcttga ctcatacctt ttccatcatc ttcaacaatt atatagcaaa aatctaaatc
      601 agacttagag gtaaccctta tattacatcc ttgttggtta tgagttatac tattttgtat
      661 taaattacta atagcccttt ttaataattt tttatctgca tctataataa tatcttcatt
      721 tagtatatca agttcaacgt aaaagtcatt atccaaatta ttatttataa actcagaaat
      781 aagctctctc aaaaacgcag aaactctaat cttatcacaa ttcaatgctt gcatattata
      841 ttcaagttta gacactaaat ttaggtcatt tactaattct ctgattttaa tcccttgatt
      901 gcatataata gacacttgct cttgttctct atttgaaagg ctactgcttt cttcaagttc
      961 tccagcatat ccaattatca ttgaaagtgg agttcttata tcatgagata ttcctgcaat
     1021 ccagttagac ctagcttcct ctttcttcct aagtttttca tctttcattt gcaactcttt
     1081 agatacagta ttaatagatt ttgaaatatc acttagtatt cctttttctt gtaaaagtac
     1141 tggttctcct tttgacataa gctttatgcc agtaataatt ggttttatag atttcaccat
     1201 ttttaaacct atgaatagag ctattaaaaa agtaactatt gcattaacca gaaacatacc
     1261 tattattaac ataggaaatt gttctatttc cttaaccgta taagtaaggt taaacttagt
     1321 gtacttgtct tttggataac ctgttactaa tattccattt ggatgttccc atacatacac
     1381 aggatagtcc tttaaaaaga accttgaaaa ctttgctaca tctgtcaaag tataatgact
     1441 tggaatttct tttggtaaat caaatcccca tattctatct ccattgttat caattaacat
     1501 tgcccaaact ttatttttat gtagtacttc cttactagaa tcatctaaaa taaactttcc
     1561 atttcttaaa atcaaattat cttcaatcat aatcaagttt gattttattt ctctgctatc
     1621 acttgtatct aaattatcag agtatttaga tacaaatagc atatatgaaa acagaagaaa
     1681 acctgctata tttaatacaa taaaaagtaa tgaaatcaag atacttgaac ctataaatcg
     1741 cctcaatatg tactttacac tatccaaaat taaatcactc ctcttgattt cttttattct
     1801 aatatctaaa attaattaat catcaactta tatccaagac ctcttactgt aataagatgt
     1861 ttaggtgtag atggagtttt ttctatcttc tctctaaccc ttctaacatg aaccataagg
     1921 gtattttcat atccatacat atcatctccc caaatggctt gacatagtga atcattagta
     1981 acaattctcc ctttattttc atacaacttt aaaagcaacg aatgctcttt agctgttaaa
     2041 gaaatctctt gcccatcttt ctcaacacat gcactattta aattcacaac aacagaatct
     2101 cccagtttaa atactggcaa ctcctcttct tctataggca catatacacg atttaatata
     2161 cccacaagcc ttaaagtaag ttcctttggt aaaaatggct ttactatata atcgtcagcc
     2221 cctaagccca aaccaatcaa tctatcttca tcttcacctc ttgcagagag aaaaatcact
     2281 ggtattttag atgtctctct aatttttctc ataatagaaa aaccatctcc atctggcaat
     2341 acaacatcta aaattgcaca atctggcttt acatttctaa ataccctaac agcttcctca
     2401 taacttgatg ctaaaaatac tctactaaac ccctcttttc tcaggatttc atctatcatt
     2461 tttaataatt ccttgtggtc atcaaccaca agtatttttt tatcttttat tgactccata
     2521 atcatcaccc tcctttagta taccatatac ccattttcac aactttgcct aaatagactt
     2581 ttaaggttat tgtaaggtaa atttcagcta attgaaatgt aaccaagtta ttatactgat
     2641 ataagttaaa agaatcaata agcacagatt aaaaaagggg atgaaacata tggaaatgat
     2701 aattagaaca caaagacttt caaaggaata caataaaata tttagagtaa aggatataga
     2761 cctaagagtt ccacaaggag ctgtatatgg ttttttgggt cctaatggag ctggaaaatc
     2821 taccactctt aaaatgcttc ttggacttgc taaacccaca caaggaaata ttaatatttt
     2881 aggtaaagag ttaaatgaaa aaaatagaat atcaatttta aaagatatag gttctttgat
     2941 agaatctcca tcatactatg gtcatcttac agggcttgaa aacatgacca taatgcaaag
     3001 actcttagat ttacctaaaa aaaatgtaaa tgaagcatta aaaattgtaa ggttagaaaa
     3061 tcaaaaaaat aaaaaagtta gtcaatattc tttaggtatg aagcaacgtc ttggcattgc
     3121 tatggcaata atgaagtttc ccaaattatt aatacttgat gaacctacaa atggactcga
     3181 cccagcaggg attgaggaaa ttagagaatt aataaaatct ctaccagaaa aatacgggat
     3241 gactgttcta atctctagcc atcttttaag tgaaattgac caaattgcca catcaatagg
     3301 aataataaat catggcgaac tggtttttca agatagcata aaagaacttc ataatagagg
     3361 tcaaagtcag attgctatca aaactaagaa tgttgtatct gcacaaaaac tattaaatac
     3421 taaaggatat atgtcactta tacaagatgg gtacttgact attgaaaatt taaaagactc
     3481 agatgtcgct aaaataaacg gtatgcttgt ccattcaaat atagatgtat ttagaataga
     3541 agaacataaa aaaagcttgg aagatatatt cttagaactt actggtaagg cggtgacttt
     3601 ataatgaaat accttggtct agaattttat aaacttaaaa gaaagaagat tattctgatg
     3661 acctttttat ttgtagctgt tgaaataatg tggtgtatca taaattcaaa tagagctatt
     3721 acacgtaatc cagatatgtt aggtggattt gaatattcct atatgcttat gagttttgca
     3781 agtttaaatg gtctattctt tcctatctta atatcaataa ttacatcacg tataagcgat
     3841 atagaacata aaggagatac atggaagctt ttaaaatctt ctgtgacatc tttaaatagt
     3901 atttatttat ctaaattttt atgttctgct attttggtat ctatacctgt attgatgcaa
     3961 gtgttatcta ttgtgttgtt tggacatttt agaggggtta ttgagtcaat ttctgtatct
     4021 ttacttttaa aatacacatt aggaacaatt cttgtaagta ttgctatcat tgcactacac
     4081 ctatggattg caactgtaat tccaaatcaa atgattgcaa ttacatttgg catgattggt
     4141 agttttatag gtttgacatc aggtttgttt tatcaaggta ttcgtagatt gtttatatgg
     4201 tcttattacc tagagttatc acctttaagt tatgcttatg ataatgtttt aggttcatct
     4261 gtctatagaa ttgatatgaa tttttctatt acgattttag ttttcttaat tggaatgcta
     4321 atttattata ttggaagaaa tcatttatta aaaaaagaag tataggaggg atttaaatgt
     4381 taaggaagtc agtcttggga gaaattctaa agttaaaaaa ttcttttata tggtatgtat
     4441 tattagcttt gccacttata agtgtgttga ttggttctgg taatttttat ctaaatcaag
     4501 gcatactcaa aaaagaatgg tacagcttat ggactcaagt tagtcttttt tatggggaat
     4561 ttttctttcc tatcttaatc gcaatctttt gtgcatatat ttgtaggctt gaacatatga
     4621 atcataattg gaataatgta atgacactgc cagttaattt aaaaaatatc tttttatcta
     4681 aacttacagt agtttcaata cttacagcta ttacacaagt tttctttgtt atcttttata
     4741 tagttgcagg aaatatgttt ggtttttctg gtagtgtacc taaagaattg actggttgga
     4801 ttgctaaagg ttggattatt tctatttcaa tagcttctat acaattatac atgtctataa
     4861 agattagaag ttttgcaact cctattggta ttagtctttg cctttctcta tttggtatgg
     4921 gattaatagt agcaaaacct gctgttggta tatttttccc ttattctatg ttaggtagtg
     4981 ctatgggggt tataaagcaa tctgctttaa atgttaatga cactatagtt acaatatgtg
     5041 ttgcatttat tactactata gtattttctg aaattgccaa tagaaatttt agaaagaatg
     5101 atgttatttc ttaatggtaa atgctagata ttttttggtt tgaaatttat attcttttta
     5161 gttgaaatct atatccattt tatttaaaaa tatatatctt ttatttgaat cttatatgct
     5221 ttatctactc gctatataat agatatactc ctaaaattaa aa
//
LOCUS       DAELJO010000235         5247 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELJO010000235 DAELJO010000000
VERSION     DAELJO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.237"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            168..857
                     /locus_tag="KPB88_003484"
     CDS             168..857
                     /locus_tag="KPB88_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055721.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGHAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            857..1774
                     /locus_tag="KPB88_003485"
     CDS             857..1774
                     /locus_tag="KPB88_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055722.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1847..2773
                     /locus_tag="KPB88_003486"
     CDS             1847..2773
                     /locus_tag="KPB88_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055723.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2770..3450
                     /locus_tag="KPB88_003487"
     CDS             2770..3450
                     /locus_tag="KPB88_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055724.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            3895..4596
                     /locus_tag="KPB88_003488"
     CDS             3895..4596
                     /locus_tag="KPB88_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0055725.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGKHTLDDVQETVNSSYVFDTIIDSLKEKITHNFLRNLHSSLIFNTTLN
                     SRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKILWK"
BASE COUNT         2022 a          562 c          806 g         1857 t
ORIGIN      
        1 gtatactata ttttaattct tttttcaata aagcttattg aaaagaactt taacttagaa
       61 gagtatcacc aaattgcaga tacaatatca taagcagtta tttattgaca acccttttta
      121 ataaatatat catttgaagt atactgacga aaaggagttg aataataatg gacatcttac
      181 tggttgaaga tgataagaat attcgagaaa tggtatacga ttttctgatt agtgagaatt
      241 actctgttaa aactgccatt gatggaaata cagctattga aatatttaaa aattattcat
      301 ttgatattat tttactggac ttgatgttac caaagtctag tggttttgaa gtattaaaat
      361 atatacgaag tgtgagtgtt gttcctgtta ttattactac tgcgaaagat agtgacacag
      421 ataaaatgct agggcttaat cttggtgcag atgattatat tacaaaacct ttttcaatcg
      481 tagaattgct tgcaagaatt aaagctaata ttagacgtgc aacagtgtat agcagttcaa
      541 gaaactctca agatatggtt attcagtata aagatttgat tataaacata tcaaaatatg
      601 tagtttcaaa gaatggaatt gacttaaact taacacatac agaatttgaa attgttaaat
      661 tactagcgac taatcaagga catgcttttt caaaggaaca attatacaac actatttgga
      721 aagaacctta ttatggaaat gaaaatgtgc taaatactca tattaacagg ttaagaaata
      781 aattgtctga tggtatagaa aaaaataatt atattaaaac tctttgggga attggctaca
      841 aaatggagga agaataatgg agttattttt agttgtaatt tctattgtac tatttgctct
      901 ctacttaaaa gaacgaaata aatcaaagaa aatggaaagt gattataagt atatcaattc
      961 aagacttcgt gacataacaa atgatgatga aattaattat attttagtac catctgatat
     1021 ttcgattgta aaagaaacag cagtagatat aaataaactt gttgaaaagt tttacagcag
     1081 gcaaattaat tataatagaa gtgaaaaagc gatattgcaa atatttacaa acatatcaca
     1141 cgatttacga actcccatca cagtattaaa gggttatatt gaaatgctat atcttcaaag
     1201 tcaaaaagaa gaattatctt cttcaatgag agcaactgtg gaaaaaatgc aaacaaattc
     1261 aaatgagctt gttaattcaa taaataacct gtttaatatg gcaaaaatcc aatctggaga
     1321 tatgatatta aatcttcaaa aatccaatct aacacaatta tgtcatgaga ttattttaga
     1381 gttttatgat ttgctagata aagaaaactt taatgttgaa gttaatattg aagatagacc
     1441 tatatatgca aatgttgaca cagaagcatt acaacgtata ctcaaaaatt taattgataa
     1501 tgctataaaa tatggttcag atggtagatt tttagggatt tcactatatg aaaaggaaaa
     1561 acatgttttt atagatattg aggataaagg ttcaggtatt tctgaaactg aaaaagagca
     1621 tattttcaca agagcttata ctgctgacag aaaaaaaggc aatggtttag gtttggctat
     1681 ttctcaaggg cttgtaaact ctatgggagg ttcaatttat gtaaatagta gacctaatgt
     1741 aaaaacagtt tttacggtaa aattgaaaag ttagaaaaaa gttagattta aaagagagaa
     1801 aagataattt tattttgtat actgttttta gaaaggaggg agtatgatga actatatcat
     1861 agagacccat caattaaaga aaatatataa agataaagct gttgttaatg ctgttaatat
     1921 tcatgtgaaa aagggagaaa tttatggatt tgttggacca aacggggcag gtaaaagtac
     1981 agtaatgaaa atgttgctaa atcttgttaa tccaaattct ggagaaatag ttatgttcgg
     2041 taagaaagtg gcagaaactg attttgaaat attaaaaaag attggtacta ttattgaaaa
     2101 tccgtattta tatgagaatt tgactgctaa acagaatctt gatttacact gtgaatatat
     2161 gggttattat aacaaagaac atatatcaga ggtattggaa tgtgtcggat tgtcaaagca
     2221 atcaaacaag aaagtttcaa aatattcttt agggatgaaa caacgtcttg caatagctag
     2281 agcaattctt actaaaccag aattattgat tcttgatgaa cctataaatg cactcgaccc
     2341 agaggggata agagaaatgc gagaattatt ccgtaagtta aatacagatt atggaataac
     2401 tatatttatt tctagtcata ttctttcaga ggtagaacaa attgcggaca ctattggaat
     2461 tattcaagat ggtaaactta ttaaagaaat ttctatgtca gatatacaca aatatcaaac
     2521 agattatgtt gaagtagatg tagataatgt ggagcttgct ggatacttgt tagaaaaaga
     2581 atttggaata aaagacttta aaattacttc ggaaagttgt attgaaatat atgatttacg
     2641 aaaagatgta aaagaaatat ccaaaatatt tattcaaaat caaattggta taaatagcat
     2701 tggacgtaaa caaagttctt tagaagatta cttcttccaa actacaggta caggaggtaa
     2761 agaaacacta tgatacattt aatgaaacta gagctaaaaa aagttgggct aaaaagatat
     2821 tttttatttt caatgctggg gattttctta tgtatgtttt ttgtctttac tggattgaat
     2881 gataaatcta cttcaatata tgattatgat gttacattta ggacaatcag tttatttttc
     2941 tgtttttact atattatatt atttgctgtg ttgaatgtgg catatataat aaatgaatat
     3001 acaaataaaa caatcttgat tttattctct tatccacttg accgtaaaaa acttatttta
     3061 tcaaagttac ttttgattac gactcttatg atatcatcaa tgatagttgg atatatctgt
     3121 tgttttacat tcgttgttgt gttggataag tattttaatt tagttattgg agattttcat
     3181 atttcaattt tatcttattg gataccagga gcaattaaaa gtattattgt attttgctca
     3241 ttaggtatat ggacattagt tgttggaatg ataagaaaat caatacctgt tacccttgtc
     3301 agttcaatta tttttatcaa tttaagacaa tttattttag ctggtacaaa tacaactcaa
     3361 gacagtttga tttctgtttt tattatagtt ggtataactg tgattggtat atattatata
     3421 ctaaatcata aagttactga aatcgactag atactgtgga taggaaggta gttattaatt
     3481 gtatcttctt gggataataa tgatttgcta ttatatcagc tattattaat attattagag
     3541 ttttaataat attaataata gctatatatt ttttcttctt atttataaat ttaatgattt
     3601 atagtcataa aatgaactct aagaagagat aaaaatacgt tttatatgtt tttagattaa
     3661 tataaaatat cacaatgaaa tgttgtacaa agtgatatat aataattata tgaactataa
     3721 aattgactaa acttattgac taataaatta gatgaaaagt tgagacttac tgaaaataaa
     3781 tacaatgaaa tatatactac aagtattaaa ttaaatctga actttacata ctattagaat
     3841 aagtattata agattataat gatttaaaaa tattaaaata attgagatga taacatgaac
     3901 aattcctttt tagataaatt gattgagaca aaagaactta aaaattcact ttataatgtg
     3961 ttaaaacaca attttcttta tcattctaat aagatagagg gcagtacttt tactactgaa
     4021 gcattagcac ttttactcga taagaatgtt gttacaggaa aacatacttt agatgatgta
     4081 caagaaactg ttaattctag ttatgtattt gatacaataa ttgactcact gaaagagaaa
     4141 ataacacata atttcttaag gaatctacat tcaagtttaa tcttcaatac aaccctaaat
     4201 tccaggggga tgtctggtat atataaaact atacccaata tgatactagg aactgatgta
     4261 agcatagcac aaccatttga agttgagcca aagcttgatg aattaattga atggtactat
     4321 agtcagagtg aagtatctat taaagttata gctgaatttc attatagatt tgaattgata
     4381 catccatttc aagatggtaa tggtcgcata ggtagatttg ttatgctaaa acagatgtta
     4441 gaaaataatc ttcctataaa aattgtatct tgggatagtg aggaattgta tagaaactcc
     4501 ttaaattctt gtagtttagg taattatgtc cctttaattg aatatttatc aagtttagaa
     4561 gattttagag aagtatataa aatattatgg aaataattgt ttacagcaaa aaaagttttt
     4621 atataaatat atttttatct atgctattta acgctgattt cttagttatt cttagcttgg
     4681 actatatcca ctattttaac tatataaaat agtggatata gtttaactat ctctttataa
     4741 ctatttttat gtatcttagt tgtttcttaa tatggggaca gttcataaaa ctaaaagtga
     4801 tttttaattt tatcatttat aatttaccat tacatcagaa aaatgcttat cattagaatt
     4861 ttcataaaag gtacctattt ttttcaatta actaaatcaa ttctattagt cttaatcttt
     4921 cgtatttcaa attgataata taagaaaata aaaaataaaa aattttaaat tttttattta
     4981 ttgcgaaaaa aataaaaagg tgctatactt tttgtaacat attattcaaa ttattaagag
     5041 atagtaaaca tttatagttt aatttgtagt acactagcat gatttgcaga acaatgaatt
     5101 aatctatagt ataatagttt aatttaaagt acaacgattt aatctatagt acactagcat
     5161 gatttgaagt ataacgattt aatctatagt acaataactt gatttgcaat acaatgaatt
     5221 aatctatggt ataatagttt aatttga
//
LOCUS       DAELJO010000236         5234 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELJO010000236 DAELJO010000000
VERSION     DAELJO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.238"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            58..261
                     /locus_tag="KPB88_003489"
     CDS             58..261
                     /locus_tag="KPB88_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055726.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            425..1579
                     /locus_tag="KPB88_003490"
     CDS             425..1579
                     /locus_tag="KPB88_003490"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG0055727.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1592..2596
                     /gene="rseP"
                     /locus_tag="KPB88_003491"
     CDS             1592..2596
                     /gene="rseP"
                     /locus_tag="KPB88_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG0055728.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2779..3834
                     /gene="ispG"
                     /locus_tag="KPB88_003492"
                     /gene_synonym="gcpE"
     CDS             2779..3834
                     /gene="ispG"
                     /locus_tag="KPB88_003492"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG0055729.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4182..5006
                     /locus_tag="KPB88_003493"
     CDS             4182..5006
                     /locus_tag="KPB88_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055730.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2039 a          526 c         1000 g         1669 t
ORIGIN      
        1 atgaaaattt tccaaaatag ataaaatgtg atagaatata ttttgcgagg tgataacatg
       61 atttcatacg ctccattatg gaaattgtta attgacaaaa aaataaagaa aatggaattt
      121 gtaaatatat caggtataag tatttctgtt ttaggcagac taggaaatga taaatctgtt
      181 tctatggata caatggagaa gatatgttta gcactagact gtaaaataga agatgttgtg
      241 gaaataaaaa agggcattta ataatactag aatattttat aatattccac aaaatacaaa
      301 aaataataga ttaattttaa tctgtggaat aatatatgaa atttctatga atagtaaaaa
      361 taattgtttt agtagtgtta taatgttttg acagggtata tttattatta gaaaggatta
      421 ttatatgaaa aagatatcaa ttttaggttc tacaggctct ataggtaaac aaactttgga
      481 tgtagttaga gaacatagag ataagtttga gatagttgca atatcagcta acagtaatat
      541 tgaattattg ctagaacaga tagtagagtt taaacctaaa tatgtaacag tttttgagga
      601 aaataaagca ttgaaattaa aagaaatgtt gcctaaaaat atagaaatag aagttttagc
      661 aggaatggaa ggattaaaaa taatatcatc acttgatgaa gtggatgtcc ttttaactgc
      721 tgtagtaggt atgataggat tagtcccaac tctttgtgct ataaagaagg gaatagacat
      781 agcattagcg aataaagaaa cgttggtaac tgctggtgaa cttgtaatga aagaagcaga
      841 aaaatacaat gtaaatattc ttcctgttga tagtgaacat agtgctatat ttcaatgtct
      901 aaatggagaa aataaaaaaa atatagaaaa gataatactt acagcatctg gtggtccatt
      961 tagaggaaag aaaaagggag aacttgcaaa tataactaaa aatgaagctt tgaagcatcc
     1021 aaattggagt atgggaagaa aaataagtat tgattcttca acgcttatga ataaaggact
     1081 tgaagtgata gaagctagat ggttatttgg agtagaacaa gaaaatatag atgtagtagt
     1141 tcacccacaa agcataattc attcaatggt gcagtataca gatagctcaa taattgctca
     1201 attaggatgt ccagatatga gactaccaat acagtatgct cttacatatc cagatagaat
     1261 ggaaagtagc tttgaaagaa tgaatttttc aaaatttagt actttaactt ttgaagaacc
     1321 agatttagaa acttttccat gtttaaaatt ggcatatgag tgtttaaaaa tgggaggaac
     1381 ttattcttct gttttaaatt cagcaaatga agttttagta agtgaatttt tagaggataa
     1441 aattggtttc tatgacatac catattacat agagaagact ttagaagttc atagtagcat
     1501 aagtgaacca acattagaag aaatcttaga aacagataga tggagtagag cttatgttgc
     1561 aaatctgata aaaaaatagg aaggtgaatt tatgactata atagccgcat taatactatt
     1621 tagtataatt gtattaatac atgagttagg acattttata tttgcaaaaa gaagtggtat
     1681 caaagtaaat gagttttcta taggtatggg gcctaagata tatagtgtaa aaaaagatac
     1741 agagtattct ataagagcac ttccaattgg tggatatgtc agcatggaag gtgaagatga
     1801 agaacaaata agtccaaatt cttttggaaa caagtctata ttacaaagat ttagtactat
     1861 tgtagcagga ccaatattca atataatatt agcagcaata cttttagtcc cagtattttt
     1921 gtatataggt tctccaacga ctaaattagg aaagataatg ccagatactc ctgcacaggc
     1981 tgtagggctt caagtaggag ataagataaa taaaattaat ggtaattctg ttaagacttg
     2041 ggatgaagtt gcaaatataa taaatacttc ttctggtgga gaattaaaac ttagtataac
     2101 tagagatgga agtgataaag ttgtaaatgt aacacctaaa aataacaatg gaaagtatga
     2161 aatagggatt caaccacaaa gagaaaaaga ctttttgggg tcaatagtaa atgcatgtaa
     2221 aactactgta gatatgacaa aacagatgct aacatttcta ggtcaaatga ttacaggacg
     2281 tgtaccagga gggattggca atgctgtagc aggaccagta ggtgttattg gtatggtatc
     2341 agatgctgct cgtacaggca tcataaatgt agtgtattta gcagctgtca taagcttaaa
     2401 tttaggtata gtaaatctat taccaatacc agcacttgat ggatggagaa tacttatgct
     2461 gttactagaa gctgttagag gtggtaaaaa actcgaccca aataaagaag gcatgataaa
     2521 tgttgttggt tttggagctt taatgttatt tatgcttttt atcacatata aagatatact
     2581 aagattattt caatgattta gttttttaga gattttaaga aaataggcta tgtagtttta
     2641 aataagaata aattaatttt tatatatgaa atttattctt atttaactag atagcttttt
     2701 aatttataat atacatgtag gttataatat tttaatattt aaagagattc aatataaaat
     2761 aatatataga ggtggaatat gagttacgag agaagaaaga cgagagaagt aagtgtagga
     2821 actgtaaaaa taggaggaga aaatcctata agtatacaat ctatgacaaa tactgataca
     2881 agagatgcag atgctacaat agctcaaata aagagattag aggaagctgg ttgtgatata
     2941 gttagagttg ctgttcctga tattaaagct gctaaaaata tagcaaagat aaagtctagt
     3001 gtaaatatac caattattgc agatatacat ttcgattata agctagccct tgaagctata
     3061 gaacaaggtg tggatggaat aagaataaat cctggtaata ttggaagtat agagagagtt
     3121 aagatggtag ttgagaaatg caaggaaaga aatttaaaaa ttagaattgg agttaatgga
     3181 ggttctttag agaaagaact tttaaaaaag tatggctctg caactgctga agcattagtt
     3241 gaaagtgcaa tgggacatat taagattctt gaagacttag attttcataa tatagttata
     3301 tctctaaaat catccgatat ttataaaact gttgatgcat atgaattaat atcaaaaaaa
     3361 gttgattatc ctcttcatat tggaattaca gaatctggaa gtgttcacaa aggaacaata
     3421 aagtcttcta taggggtagg agcacttctt cttaaaggaa ttggagatac tgttagaata
     3481 tctttaacag gcgaccctgt tgaagaggtt attgttggta aacagatttt aagaagtttg
     3541 ggacttttaa atgataagat taaagttata tcatgcccta catgtggtag atgtaatata
     3601 gacttaatta gtgttgtaaa tgaggttgaa gaaaagattg gtagtatgga aaaaaatatc
     3661 actgttgcaa ttatgggctg tgctgtaaat ggacctggtg aagccagaga ggcagatata
     3721 ggtattgctg ggggtaaagg tgaaggtctt ttatttaaaa agggtgaaat agttagaatg
     3781 attgatggta acaaattagt tgatgagttg ctagaagaga ttgaaaaatt atagtataca
     3841 attcatatat atattaattg actagatttt taaatacttt tgtaataagt ttttatttac
     3901 agtatttaat tatctagtca ttttttattt tataaacttc attaaatgta agaaacttat
     3961 attctaattt gactatatat gtatgagtta aatcatattg taaatttata aaattaaaaa
     4021 aagatttaat atagtgaaaa tttccatcaa tagtattgaa acttgatagt aactatgata
     4081 ggatatagtt actagaacag ttggtaaatt atgttaacat agattttaat gttttggtaa
     4141 attgttttat tataagttaa tagaaaaaga ggtgaagaac tgtgagatat aaaaaaatat
     4201 ctagcttacc tattttagtt gttgctttgc tgattactgg aacgacaaca acattttctc
     4261 ttagtacgac caattcagaa aaaacaaatg tatacttagc aaatacggta agtactgtca
     4321 caaagaaagt atcagatatt caaagtagta aaggtcttgt ttataatgag aatattccat
     4381 tatttataaa taatagcaaa atacagtatg atgatactcc atatcattgg cctagtaatg
     4441 ttataagctt aacaaatagc agtgaaaagg caattatgga ttatgaaatt acatgcttag
     4501 catatgataa aaatggaaaa ccacttgagc tatattggga tgctcaaaat gtggcagcag
     4561 atggcgaagt aggaagtgtt ggatttagcc cagctggtgt agactatggt attgttacag
     4621 gcatatctcc tgtgtctcca aaatcatatt cacatactta tagaaaaatg caacaatcac
     4681 caccacagga tataataagt atgtttgaaa aacaacaagg caaggcatgg gtagaaaatt
     4741 ggttgaaaga atggaaacaa atggagaaag aatatgcaaa gcaaaatgca atagcaccag
     4801 gtaagaatca aaatgatgcc tttctattgt ttgataaatg gaaacaatca actggagagc
     4861 atggtgtaaa gtacattata tcttgtgtaa aacaagtaac atttaatgat ggtagtgtat
     4921 ggaaaaactc agcatatgag aattggttga aaagttttca agggaaggaa gtttcaaata
     4981 gtgtattaga aaattactat aagtaatgtt taatgatagc aaaatgagta cgaatttaat
     5041 taatgttaat ttaattaaat aaggactatt ttgtttaaaa atatcagtgt tacaaggaaa
     5101 tttttattgt gacaaaatga tatatataca taagattata agcatgtgtc aagttttgat
     5161 acatgctttt ttattttgaa tatatgtttt atataaattt atgatttttt agttatgtaa
     5221 ttttatatgt gaaa
//
LOCUS       DAELJO010000237         5227 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELJO010000237 DAELJO010000000
VERSION     DAELJO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.239"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..320
                     /locus_tag="KPB88_003494"
     CDS             <1..320
                     /locus_tag="KPB88_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0055731.1"
                     /translation="IEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVK
                     VGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRIITI
                     QNK"
     gene            447..1616
                     /locus_tag="KPB88_003495"
     CDS             447..1616
                     /locus_tag="KPB88_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG0055732.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1779..2321)
                     /locus_tag="KPB88_003496"
     CDS             complement(1779..2321)
                     /locus_tag="KPB88_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG0055733.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPNLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2469..2753)
                     /locus_tag="KPB88_003497"
     CDS             complement(2469..2753)
                     /locus_tag="KPB88_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0055734.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2816..3580)
                     /locus_tag="KPB88_003498"
     CDS             complement(2816..3580)
                     /locus_tag="KPB88_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0055735.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3862..4128)
                     /locus_tag="KPB88_003499"
     CDS             complement(3862..4128)
                     /locus_tag="KPB88_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0055736.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4170..4979)
                     /locus_tag="KPB88_003500"
     CDS             complement(4170..4979)
                     /locus_tag="KPB88_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055737.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLALQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1831 a          697 c          737 g         1962 t
ORIGIN      
        1 gcatagaaga tttacaagta aagaatatga ttagaaatcg taaactatct cgcttaattt
       61 ctgatgtgtc atggtcagag tttattcgtc aattagaata caaggctaat tggcatggta
      121 gacaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat aaatgtgggt
      181 ataaaaacga ggaagtgaag gacttaaata taagagaatg gatttgtcct agttgtaatg
      241 aaactcacga tagagatata aatgcaagta taaatatatt aaaagaagga ttaagaataa
      301 taacaattca aaataaataa cgtatgtaag aaccgtagga actacgggga tagcctatgg
      361 agaattagta agacatattt tagtatgcaa aattctgtga agcaggaacc ctgtgacttt
      421 agtcatggga ggttcagggt agaaagataa atgaactttg taaagatgaa gttgtagagt
      481 tttgcaatag aataaaaggt aaagtggaaa gtgttgcaat aagtggagta tttgctccag
      541 taaataaaca acaagaagaa gaagttgcaa aatgggttag agaaatttta ggggatattc
      601 ctatatcttt atcaaatgaa ataggtaata taggtatatt agaaagagaa aatggagcta
      661 ttttaaattc tgcattgtct caagtaggta aagctgtatc tttaggattt gaaaaagcac
      721 ttaaagactt aggtatagat gctcaaatat atttcagtca aaatgatgga acattaatga
      781 atttagagta tacaatgaaa tacccaattt ttacaattgg atgtggaata acaaattcta
      841 taagaggagc ttccttctta tcaaaagtaa aaaatgcaat agtattagat gttggtggaa
      901 ctacaagcga cttgggtgta ctatataatg gattccctag agaatcatct attccagtta
      961 caattggagg agtacataca aactttagaa tgccagatgt tttaactatt ggtcttgctg
     1021 gtggtacatg tgtaaaaggc gaaaaagata atataaagat aggtcctgaa agtgtgggat
     1081 atagaataac tgaagatgca ataatatttg gtggagacac tctaacttta tcagatgttg
     1141 ttacaaaatt agatatggcg tttgaagata gaaaaagcaa tgttgaaaac ttagataaag
     1201 agtattgtaa tgaggtatat aagaaggtta ttttaaaatt agaagatgct gtagaccaaa
     1261 tgaagactag tcaagatgat gttgaattgg ttttaactgg tggaggaagt ataattgttc
     1321 ctgaaaaact aaaaggcata agcaatgtta taagacctcc tcattatact gctgcaaatg
     1381 ctattggtgc agctttagga caaataagtg gagatgtaga aaaagtatac agtttagata
     1441 aaatgtcgag agaagatgcc atagaagatg caaaaaaaga ggctgtaaat aaagctctaa
     1501 aagctggtgc taaagaagat actatagaag tattaaatat agaagatgtt ccacttgcat
     1561 accttcctgg taatgcactt cttataaaag taaaagttgt tggtgattta atttaaagaa
     1621 taaatataag tatttccaga attatataaa tatttctgga ctcattttta aacttatttt
     1681 atcaataaaa acagcataac ttttgactat attcttataa gtttccgaac agccgatata
     1741 tacctatttt ttgaggttta tcggctgttt gttatctttt atcgcattcc aactattttt
     1801 cttaccgtat ttgctgtacg aattgtaatc ttatcactta catttgaact ggcagtcttt
     1861 gaccaccaat ttgtcttttt ataatcttta cgactaaaag accaaaagat agcattttta
     1921 taatgatata ctttttcata ctcttcttta ggatttccaa cctcatcata aaactcgtca
     1981 taagataagt taggaaacat aaaaataaga ttatcatata tttctttgtt gttatctccc
     2041 caccaattag gagcattatt tagtatatct tttaactcct cttgtgaaat aataaaaact
     2101 ggtatttcca attcaaactg tttttttatc attaatctta ttgtatttga gataaccttg
     2161 ctgtcatcta catcacttaa aaagacaaca ttaccactat taaggtatgt agaaacttct
     2221 gaataaccaa gttctacaaa gcttgctttt aattcagaca taattatctt gttcttacca
     2281 cttatattga tacctcttaa taatgcgata taacttttca tataataatt tcacctctaa
     2341 acatttacat ttttctatgt agtacaaact tgaatttgca tcttttataa ttctttcaaa
     2401 acttcttcat atgtaatttt tttaatataa aaaaatcggt gcttaatttg taccgatttt
     2461 tgtttttctt agtaaagaac tcgttttttt gcttctgcta tagctctagc agcacttttt
     2521 gcttgcattc ttttttcttc ttctttttta aattcattta ttgtttcatt tagattctta
     2581 tcatgttcat ttttttcttt atcttcgttt tctctgtata ctaatatata atctattatt
     2641 gattcgttta ttatttgaga tatactttta ttttcattaa ttcctattct cttaacttcc
     2701 ttaagtagag tctcatcaat atgaaccgtt gtttgctttg tattttttgc caccttaatc
     2761 acctcttaaa aaaaattcta acattaaaat tttttttagt caataaattt taatattaaa
     2821 attctttaaa tgttttattt gattttctaa aattttctat tgtatttatc atcttatttt
     2881 ttatattttc acctagtaca gaagacttta gtctaggatt ttcgatatgg tcataaattg
     2941 gttctaaaaa ttcgacttcg acaggaacag agttaatttt accaataggt tggtatcctt
     3001 cataagtatc ttttgaattt tttattacta gtggaacaat agggcatttt gctttatatg
     3061 ctatctttaa agctccactt ctaaactccg aaaccaatga atttggttct ttaatccatg
     3121 ttaaatctcc ttctggaaac actgccatac tttgacctgt aagtatattt tgagaggctt
     3181 gggctatact ctttatacct tcacgattgt tttttctgtt tacatataca catcttagta
     3241 attttgccca ttctttaaat ataggtatat ttctccaaac tggttcatct gcaattacac
     3301 atccaattgg tctttctaca cttgctgtta gtatgaagct gtcaagcata ctagaatgat
     3361 ttacaacaaa tagaagtggt tcttttggca gtgtttcctt accaattata ttaagctcta
     3421 tgttcactat atctaaagat tttttagctt gtttttgcat aaattcaaat ttttccttta
     3481 agctaaattt atcaggattc tttttaattt taataagcct aggtatagaa gatgtaaaac
     3541 ctataagttg atatgtagca aattttaagt aattattcat aaaattatct ccttaaaata
     3601 aaattattta ttactgtaaa gattttaaca taaaataaaa tttaagtcta ttttaattca
     3661 attttgtaac atatatttta aatagacaga gattgagatt ttatatttcc aattttagta
     3721 atcagatttt atcaataaat tactattcta ttaatttaat taaataacct ttaaatttaa
     3781 ttataaaaac tgttgcagaa atttctacaa cagtttaaaa aatttacctt acaaaaactt
     3841 attttataac aagagagtat attataaggc tagtacagcc cattttttgc actgagaaat
     3901 tcttttgcca tcatcacctt gttctttgtc atatgcaaat tgattaagca tttcacatgg
     3961 aaatgtatca catagaccac aatgtgtaag gtttttatct tcacagcaag attttacagg
     4021 acattgttca ccccaaaatg gtttctcaat ctctgtacaa cctttacaat tgactttttc
     4081 cttgtatcca cattcataac ataaaattcc acatcttgat tctatcattt taagtcactc
     4141 cttaaatata aattttatat gattgtatat caaaatttat acttttcatt gtaaaaatcg
     4201 gacaaatttt ttatataggt tgttggatta acaccacaaa tagatttaaa atcatttata
     4261 aaatgagatt ggtcgtaata accaatatct tgtgctaaag ttgcaaatgg tattttatta
     4321 ttttgtattt cttgtaatgc aaggtttata cgaagtaatc taaggtatga tttaacactc
     4381 ataccaagag aattattaaa aattctattt aaatgtcgtt cactataaca actaatttgt
     4441 gaaatgtttt taacagacat aatagagttt tgttttctag cattttcaag aattggtata
     4501 gtcatatcta ctatatttga tttcaataag tatgataaga ataacatatc taattgttct
     4561 actaattcct ttatagtact tgttctttca aaaatggaat taacctctaa gctaaataat
     4621 gtgttaaaat cttcaagtga aatttttaaa tctgtagatt ctctttgaga aagacctgtc
     4681 aaataatata ctccacctgg gcgaaactca acgaatactc taaaaagtac attttctata
     4741 tcatttttta caatagtagt ttttgttgtg gctccccaga atgcgctttc tatacctttt
     4801 ttgtcaaatt taaatatcat acatccacta gcatcaggta ttaatgtaag attttcttta
     4861 atacttgtat ctggaataga tattgtatag tgtgctatat actgtcttaa caatttatgt
     4921 ggcaaaatat atgtatactg tttgcaggta tttaaaatgt tattgattat tttattcata
     4981 agtatatcct cctgatatat aaaaaataag tatactaatt tattttttat ataataaacc
     5041 agttttgttt taattgaaat attctaataa gatattttgt taaatttaga tacaataatt
     5101 gtaaagtaaa atctcaaaat atacaattat tataaatcat agatatttat taaataaata
     5161 tattaaagaa gaaggaatat aaaacaagat tagaaaataa tagtgttact ttcataataa
     5221 atacata
//
LOCUS       DAELJO010000238         5147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.240, whole genome shotgun sequence.
ACCESSION   DAELJO010000238 DAELJO010000000
VERSION     DAELJO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.240"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            139..2337
                     /gene="slpA"
                     /locus_tag="KPB88_003501"
     CDS             139..2337
                     /gene="slpA"
                     /locus_tag="KPB88_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG0055738.1"
                     /translation="MNKKSLAVVMSAVTVIGSAAPVFAAAEDTFPEGKSTVSSSKYSD
                     IERILKNYVKAGKTVNVNFYDKKGVSKGVSISGLKENQISYVKDEVKDLKSGESAKIV
                     VVGDKGEPAKYTDAELKELDGDAVVEQVKKATAVKFVDQDGNTLDPTKPEDKVKLQKY
                     DTAAQPSAGFVDATPEDYLQIIKDSVTKSKYTNKAGNAATGTVAITAALPAASNDGEL
                     QITVGTNSPAKTTLDTAVTTALDGTVVKFDFTKPIITLDTTTGASKVSFEKTDEIVGA
                     TGSEVTVIAASDETVTISGDAKEKAQTLAKKYVFKDSELENAYKTVTASDFEKTDNDY
                     YEVVLYPTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRT
                     YNNSYSNNSVLAGDDRIETAIEISKDSYNADKGVEGNYVEANEVVLVGSQSIVDGLVA
                     SPLAAVKNAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEV
                     ANELKDMGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNK
                     EMPIVVVDGKGKDLSADAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGD
                     DRQDTNAEVIKTYFEKDNDDSVVKNGVKNFYVAKDGSTKEDQLVDALAIAAVAGKYEA
                     PIVLATDSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            2573..4918
                     /gene="secA"
                     /locus_tag="KPB88_003502"
     CDS             2573..4918
                     /gene="secA"
                     /locus_tag="KPB88_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0055739.1"
                     /translation="MSVLDTILDKADEQEIKRLNVIVDKIESLEKDIENMSDEELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRKLNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATVTFQNFFRLYSKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     TNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKIIEMEKIILLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
BASE COUNT         2113 a          534 c          988 g         1512 t
ORIGIN      
        1 ttttgcataa attattgaaa ttctatttta tatagtctat cataagagtg tgagaaatat
       61 ttggttatat gaacatggaa ttttaatgta cataattaaa gagatgtaat taatataatg
      121 ttgggaggaa tttaaggaat gaataagaaa agtttggcag ttgtaatgtc tgccgttaca
      181 gtaatcgggt cagcagcacc agtttttgca gcagcagaag atacttttcc agaaggaaaa
      241 agcacagtat ctagtagtaa gtatagtgat atagaaagaa tacttaaaaa ttacgtgaaa
      301 gctggtaaaa ctgtaaacgt taatttttat gacaaaaaag gtgtgtcaaa aggtgtaagt
      361 ataagtggtt taaaagaaaa ccaaataagt tatgttaaag atgaagtaaa agatttaaaa
      421 tcaggagaat ctgctaaaat agtagttgta ggtgataaag gtgaacctgc aaaatatact
      481 gatgcagagt taaaagaatt agatggtgat gcagtagttg aacaagttaa aaaagcaaca
      541 gcagttaaat tcgtagatca agatggtaac acactagatc ctacaaagcc tgaagataag
      601 gttaagttgc aaaagtatga tactgctgct caaccatcag ctggatttgt agatgctaca
      661 ccagaagatt atttacaaat aataaaagat tcagtaacaa agtctaaata tactaataaa
      721 gcgggcaatg cagcaacagg aactgtagct attactgcag cacttccagc agcaagtaat
      781 gatggagaac ttcaaattac tgtaggtact aattcaccag caaagactac tctagataca
      841 gcagtaacta ctgctcttga tggtactgta gttaaatttg atttcactaa accaattata
      901 actttagata ctactacagg tgcttctaaa gtgagttttg aaaaaacaga tgagattgtt
      961 ggagctactg gttcagaagt tacagttata gcagcttcag atgaaacagt tactatatct
     1021 ggagatgcaa aagaaaaagc acaaacttta gctaaaaaat atgtatttaa agacagtgaa
     1081 ttagaaaatg catataagac agtaacagca agtgattttg aaaaaactga caatgattat
     1141 tatgaggtag ttctttatcc aacaggtaaa agattaaata cagcttcaac atatgcatca
     1201 tctaactata agccagaatt acctacaagt gatagagtag atacacctgc tataataaca
     1261 ttaagatcta ctaataagaa taacttaaaa tcagctttag atgaattaag aacttataat
     1321 aattcttatt caaacaattc tgtattagct ggtgatgata gaatagagac tgcaatagaa
     1381 ataagtaaag attcttataa tgcagataaa ggtgttgaag gaaattatgt agaagctaat
     1441 gaggtagtat tagtaggatc acaatcaata gtagatggtc tagtagcttc acctttagct
     1501 gctgttaaaa atgctccatt attgttaact tcaaaagata agttagattc atcagtaaaa
     1561 tctgaaataa agagagtaat gggattagat gacaaaactg gaataacatc taaaaagact
     1621 gtttatatag ctggtggaga gaattctgta tctaaagaag tagcaaatga attaaaagat
     1681 atgggattaa aagttgaaag attatctggt gatgatagat acgctacatc tttagaaatt
     1741 gctgatgaaa taggtttaaa ccataataaa gtatttgtag ttggtggtac tggattagca
     1801 gatgcaatga gtatagcatc agttgcttct aataaagaaa tgccaatagt agtagttgat
     1861 ggaaaaggaa aagatttatc agctgatgca aaagacttta taggaagtgc ttatgtagat
     1921 ataataggtg gaaaatctag tgtctctgaa gacatggaag atgctataga tgatgctaca
     1981 ggtaagagtc cagaaagagt tagtggagat gatagacaag atactaatgc agaagttata
     2041 aaaacttatt ttgaaaaaga taatgatgat agtgtagtta aaaatggtgt taaaaacttc
     2101 tatgttgcta aagatggttc tacaaaagaa gatcaattag tagatgcgtt agcaatagct
     2161 gcagtagcag gtaaatatga agctccaata gttttagcaa ctgatagttt atcttctgat
     2221 caatcagtag caataagtaa agttacaaat tctgatgatt ctaagaagct aactcaagtt
     2281 ggtaaaggaa tagctgattc agttattaag agaataaaag atttattaga attataagat
     2341 aaaattaaaa tatacttaag agagaacttc tttgaagaag ttctcttttt tataaaaaga
     2401 aataaaaaaa ggaactaaaa atagttcctt tttttgtgag aaatacctaa ataattaaag
     2461 gtgtaattta tttaagtatg attattatac tataaataaa aataattgtc tactattcta
     2521 taattttaag gtataatagt aattgtatga taaaaaagga ggtaaattaa atatgtcagt
     2581 tttggataca atattggata aagcagatga gcaggaaata aaaaggctta atgtgatagt
     2641 tgataaaata gaatctttag aaaaagatat agaaaatatg tctgatgaag aattaaaaaa
     2701 aatgacagac ctatttagag atagattaaa aaaaggagaa actttagata gtttattacc
     2761 agaagcattt gcagtggcaa gagaagcttc aaaaagagtt ttaggaatgc gccaatatag
     2821 ggttcaatta attggaggca ttgtaattca tcaaggaaaa atagcagaaa tgaaaactgg
     2881 tgaaggtaaa actttggtag aagttgcacc tgtttatcta aatgctttaa caggtaaagg
     2941 tgtgcatgtt gttacagtaa atgactatct agcacaacgt gataaagagc taatgcaacc
     3001 agtgtatgag tttttaggta tgtctgtagg agtaattatt gcagatctta tacctgaagt
     3061 tagaaaagtt caatataatt gtgatattac ttatggtaca aataatgaat ttggatttga
     3121 ttatttaaaa gataatatgg taactaaaaa gtttgataaa gtacaaagaa agttgaattt
     3181 tgccatagtt gatgaagttg actcaattct tatagatgaa gctagaactc cacttataat
     3241 tgctggagag ggtgatgaag attcaaaact ttatgaatta gcaaattcat ttataaaaac
     3301 aataaaagaa gaagattatg aattagatag aaaagataag accgtagctc taaaggaaag
     3361 tggtattgat aaggcggaat cattttttgg gattaaaaat cttacagata tagaaaatat
     3421 agaattatat catcatataa atcaggctct taggggtcac aaacttatgg aaaaagatgt
     3481 tgattatgtt gttgttaatc aagaagtaat gatagttgat gaatttacag gaagagtaat
     3541 ggatggaaga agatatacag atggcttaca ccaagctata gaagcaaaag aaggggtaga
     3601 aataaaagat gaatctaaaa ctatggctac agttacattt cagaacttct tcagattata
     3661 tagtaaactt tctggtatga cagggactgc taaaactgaa gaaggagaat ttgaatcaat
     3721 ttataaatta aatgttgtac aaattcctac taacaaacca attataagag cagatcttca
     3781 tgataaagtg tttagaacag agaaagaaaa gtaccaagct gttgtagaag aagtaataag
     3841 aatacataaa acaaaacagc caattcttat tggtactgtt tctgttgaga aatcagaaat
     3901 gttatctggt atgcttaaga aagaaggaat aaaacatcaa gttctaaatg ctaaggaaaa
     3961 tgataaagaa gcattgatag tgtcaaaggc tggtaagttg ggttcagtaa ccattgcaac
     4021 taatatggca ggtagaggaa cagatattgc tttaggagca ggtgataaag aagaagaaag
     4081 tagagtaaag gaattaggag gactttatgt aataggaact gagagacatg aatctagaag
     4141 aatcgataat cagcttagag gacgttctgg acgtcaaggt gatccaggaa catcaaggtt
     4201 ttttgttagt cttgaagatg atgtaatcaa attgtatggt ggaaaaagta tagagaaact
     4261 aatgaaacga acaaattcaa ataaaaataa ttctattgaa agtaaatctc ttacaaaggc
     4321 aatagagaga gctcaaaaag gggtggaagg taaaaatttt gaattaagga aaaatgttct
     4381 taaatatgat gatactataa atgaacaaag aaaagtaatc tataatgaaa gaaataaagt
     4441 tctaaatgat gaagatatac aagtagatat tcaaaacatg gttaaagata taataaaaga
     4501 agctgctgct gaatatttaa caaaaagaaa gagggattac tatggatatt ttaagcatct
     4561 ttataataca tttatgccag ctgatacctt actaatacct gacttagata ataaaagtat
     4621 tgaggatata attgatagta cttatgaaat atcaaaaaga gtttatgatt taaaaaagat
     4681 ggtagtaggc cttgacaaaa taatcgaaat ggaaaagatt attcttttaa aagtagttga
     4741 tcaatattgg gtagaacata tagatgctat ggagcaatta aaacaatatg taggatttaa
     4801 atcatatgct caaaaagatc ctttcaaaga atatgctatg gaaggttatg agatgtttga
     4861 atcattaaat aaaaatataa gagagacgac tatacagtat ttatataaat ttaattaaga
     4921 aatttaaact gtataggaaa aagaggctga taatcagcct ctttttctat gtgagaaata
     4981 cggaaaaaag agatgcttga aatttctttc ttccatttaa tattatacta taaagaagat
     5041 caaaagtcta ttagttataa actggattat taaaaaaaat tacaattttg ttactttttt
     5101 gtatatgtaa atattaaaaa aataaaatat aagaaaaagt ttatatc
//
LOCUS       DAELJO010000239         4951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELJO010000239 DAELJO010000000
VERSION     DAELJO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.241"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            578..1573
                     /locus_tag="KPB88_003503"
     CDS             578..1573
                     /locus_tag="KPB88_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055740.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1551..2414
                     /locus_tag="KPB88_003504"
     CDS             1551..2414
                     /locus_tag="KPB88_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0055741.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2411..3205
                     /locus_tag="KPB88_003505"
     CDS             2411..3205
                     /locus_tag="KPB88_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0055742.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3490..4869
                     /locus_tag="KPB88_003506"
     CDS             3490..4869
                     /locus_tag="KPB88_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0055743.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
BASE COUNT         1771 a          499 c          803 g         1878 t
ORIGIN      
        1 aataaaaata ttacagtaaa atgaccagtt ttaatatacg tattatattt ttaatttata
       61 aacgaatatt ataaatgaaa tttaaattca accttgaaga aataagctaa atgtattagt
      121 atattaatcc atgcttgaac ataaataacc tttatgatat gatatcaata ataatattat
      181 gagaggagtg ccttgatgtc ggagttatat aatggtaacc aacaattaaa tagttggaga
      241 cacatatatt ctgtataatc agaaatttca agtgtatttt taatacattt aatttttgtg
      301 tcttattctg cctttataga taactgacat atgacaacaa atagtattta aaatatatat
      361 tactttgtta aattatggtg tagtaggctt taatctgcta cacttttatt aattttgtat
      421 agtattattt tgataactat tttgaaatag agagcattaa tttttctagt ccacaggagt
      481 tttctgtttt tgtgctgtta aatataagca cagaaaaagg cagatatata cctgtggact
      541 ttttttatat aaattatgaa atatggaggt agttaaaatg agtataattg aactcaatga
      601 actgactaaa tcttatagaa gatttgagaa aaaggcagga tttatgggga gtgtaaagtc
      661 tttatttaat aggaaatata cagaaaaaac agctatatcg tcatttgatt taaagataga
      721 agaaggagaa tttgttggat taataggtca aaatggagct ggcaaaacta ctcttattaa
      781 gatgttaact ggtattattg caccgtcttc tggagaaata tctgtaatgg gttattaccc
      841 taacaaatta gaaaataatt ttaaaaaaca atatgctgtt gttatgggcc aaaaaagtca
      901 actattcttt gaattgacac cagcagatac gtttctttta tttaaagaat tatatgatat
      961 atctaatgaa gaatataaga aaaacttgga ttactttatc gaattatttg aggtatctga
     1021 ctatttaaat gttcaagtta ggacgctttc tttaggagaa agaatgaaaa tggaactaat
     1081 agtagccctt ctacataatc caaaaatatt atttttggat gaaccaacta taggcttaga
     1141 tgctatagcc caaaaacaaa ttaggtattt cctaaaagaa gtaaatgaaa caaaaggaac
     1201 tactattata cttacttctc attacatgga agatattaag agtctctgta gtagaagtat
     1261 tgttgtagaa aatagccgta aaatatatga tggttctaca gagaaactat ttaattattt
     1321 tcaaaaatat aaaaaaataa cagttagttt ttccaatgaa actaacattg tatttccaaa
     1381 agattgtaaa gttgttgaac aaacaaatca taagatatca gtaattgtgc caaaagaaag
     1441 aggaaatgaa acgattaaat ggatttttga taaatatgat gttaaagatg taatgattga
     1501 agaagaagat ataggtagta ttgtagagag gatatataag aaaggaagtg atgagtatta
     1561 tgagcaacta taatgaagca agttcaaatg attcaatgtt ttataaattt aaacagagta
     1621 ttagaaggta ttatgaagta gccaaaatta actttaaagt gcaacttatc tggagatttg
     1681 atgtggtggt taatatggtg tttacgattg taaaaatttt atttgcttat attttatgga
     1741 aagcaatttt tggtgaaaat aaaatagtct ctggttttac atttaactct atgttatctt
     1801 actatataat aagctcattt ttagcaggta ttgatatgtc aaagagtacc agtgaagaga
     1861 tatgtaatcg tgtaaaattg ggcacctttt caaagtacat gattattcca attaatgttc
     1921 aaaattattt tattgctaaa aattttggaa tgacatgttt ttattttgta tttaatatga
     1981 tttctgcatt tgtatggatt tttatattta aaatagattt tgcctttaca tcaaatccag
     2041 ttcaaatatt aatttcaatt ttgatgattt ttctagggtt tatttttatg atgaaactcc
     2101 acttctttat tgggattttg tcatttaaat ttcttgagat tagttttttt aaaatgataa
     2161 ttgaaaatat tgttgaattt attacaggaa caatgatacc acttgtactc ttaccagaaa
     2221 ctatagtaac agttatgaag gtatttccat tttattatac aacatatttg ccatctatgt
     2281 tactaatagg tagaaacagc aatgaggcaa tacaaggatt aattattcta gcattatggg
     2341 ttttattttt ttctgtagta aataaagtta tgtataaaag aatgagaaca ttatttgatg
     2401 gggtgggtat atgatgaaaa aaaatttgaa aataatattt atgttattaa aaatgaaatt
     2461 atcaaagatg atggtattta gattcgattt ttttggagca ttttttgtag atagttcatt
     2521 atttatatta caacttctaa tgtttaatag tatatattct catgtagact caataggggg
     2581 atggcaacaa ggagagatgt taatatttat aggaactttc tcattaataa atgcgataaa
     2641 tatgacgatt ttcttttttg gtatttatga tattccacga aaaatccaag aaggagaatt
     2701 agactactat attacaaaac cattagaccc actatttagg ttaacatttg aaaatattaa
     2761 tattggttca tcaccattaa ttctagcaag tatacttatt gttttatatg gaatatctga
     2821 gttaaactca gagataactt tagttatgat aataatatat attttcatgg tgttattgat
     2881 gacattactt ttttatgatg tatgtatcat acttagaaca gtttcatttt ttgttatatc
     2941 atcttctgcg ataatgagat tagaagataa tttattgcca atgaatatga aaattcctgg
     3001 tgtaatatat gaaggtattt ttaaagtatt attctattta atcttaccat atggaattat
     3061 gtcgacaatt ccgactcaaa ttttggctgg gacaataaca attaatggat taatatactc
     3121 aattttaatt gtatttttgt ttacattgtt tatgtggaga ttttggaagt taggtatgag
     3181 acattataaa agtgcaagta gttaataaat tagtaaaaat agacaatgta taactatata
     3241 taaatacgtt tcatatgaca ctaaactatg tgaacaagta tttgcgctaa aggcactgaa
     3301 agctataaaa tgattaaagt aaggatttaa aagtttcttt agtagaaata taattgtgta
     3361 aaaaagcaag attaattaaa actatatcca aataattaga tataattctt atgcagaaag
     3421 gaggtctgta aatgtctaat tgtttggaat atattttaaa aataattaat gtacagaaaa
     3481 ataaacaaaa tgagtgatgt atattattca gaaaatcatg aacaagataa tctttatgag
     3541 caagataatt tactggcaca aagatttaat ttatttactc tattgaagtt tgcatttcct
     3601 actattttta tgatgatttt tatggggttg tatacaatcg tagatactat atttgtatca
     3661 agatttatta atacaaatgc tctatcagca attaatattg tatgtccaat tattaatctt
     3721 atagttggaa ttggaactat gcttgccact ggaggaagtg ctattgtagc acgtaagatg
     3781 ggaaatggtg atattgaggg agcaaataaa gctttttctt taatcgtatt atcaggagtt
     3841 gtaattgggg taataattac tggtataggt attgtattta tggattatat cattatatgg
     3901 ttaggagcaa gtgaagtttt aattaaatat tgtcgttcat atctttttat tatcttggtt
     3961 tttgcttctg caaatatact acaagtatta tttcaatgtt tatttgtaac agctggaaag
     4021 cctcaatttg gattatggct agtgttaggt gctgggtgtg ctaatataat tcttgactat
     4081 gtgttcattg ttctgttaca aatgggtatt agtggagcag cacttgcaac tggagtagga
     4141 tacctaattc ctaccatttc aggtatggtt ttcttttcaa aaaataaagg gttacttaaa
     4201 ttttgtatac ctagactaga taaaagaata ttatttgaag cttgttttaa tggttcatct
     4261 gaaatggtgg gtcaactatc aacagcagtt actacttttt tatttaatgc tatgatgatg
     4321 aaattaattg gtgaagatgg ggttgcagct attacaataa ttatttattc tcaatttctt
     4381 ttaacttcgc tttatatagg tttctcaatg ggtgttgcac caataataag ttataactat
     4441 ggaagtgaaa ataatacgca gttaaatcat gtttttaaga tttgtatatt atttatttca
     4501 gtagcatctt tattggtatt tatcttttct atatcatgtt caccaatcat tgtagagatt
     4561 ttttctcaaa gaggaagccg agtttatgaa attgcaaggt ccggatttta tatattttct
     4621 tttagtttta ttttttgtgg atataatatt tttacctcat ctatgtttac agcattatca
     4681 aatggaaagg tgtctgcaat attatctttt ctacgtacat ttggatttat aactataggt
     4741 ttattgatat tgccaaatat attaaatata aatgggatat ggttagcagt accaagtgct
     4801 gagattttaa ccttattttt aactgttttt tgtatattta agtataagaa tagatataaa
     4861 tatatgtaga tattaaataa atagcttaga tagatatttt atgttttaat aagtaattca
     4921 attagtggta gatatatact attgaaaatt a
//
LOCUS       DAELJO010000240         4936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELJO010000240 DAELJO010000000
VERSION     DAELJO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.242"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            15..1322
                     /locus_tag="KPB88_003507"
     CDS             15..1322
                     /locus_tag="KPB88_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055744.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1408..1670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1796..2599
                     /locus_tag="KPB88_003508"
     CDS             1796..2599
                     /locus_tag="KPB88_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055745.1"
                     /translation="MKNILKKAGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2637..3248
                     /locus_tag="KPB88_003509"
     CDS             2637..3248
                     /locus_tag="KPB88_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0055746.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3280..3951
                     /locus_tag="KPB88_003510"
     CDS             3280..3951
                     /locus_tag="KPB88_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0055747.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4250..4612
                     /locus_tag="KPB88_003511"
     CDS             4250..4612
                     /locus_tag="KPB88_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG0055748.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         1978 a          455 c          876 g         1627 t
ORIGIN      
        1 aggtgggaat aaaaatgaaa gaaattgctg atgctataac atcagagtat ttaagctatg
       61 gtatcaatga agatattagt atctatgaag gtagattttg catatattta gataaaaaat
      121 atagatgtaa tgggaaaatt tattataaaa tgacacctcc aatatctata agttttaaag
      181 cagacatagg ttgtgttgaa gagatagaca atgaagatga caatttagct ttagattatg
      241 ataatgctat tcttgaagtt catggatata aaattatttc tataacaata aatactttaa
      301 gtgaatttag tgtagaaggt tatatcaatg atgactgcat aaaatctaag aactcctatg
      361 ttgaatatgt agattttaat ataataaact tagataaaat acctggaaaa ctcattaaat
      421 acaatgataa ggtatacgca ggtagaattg aatttgatat aaatgattat gttgtaacta
      481 tagacaagcg atatgattat agaaaagaat taaaatcaga attaaagtct aaatctggag
      541 caataataac tcatataggc aggattagaa gaaaagatgg tagaattttt agaacaaata
      601 atacaataaa tcttttagat aggatatcaa cagctttgag ttttatgtgt ggaagatatg
      661 tagggttttg tttggccaaa gggtatagaa gtggtaatga agtttataga atatggaatg
      721 aaaatcaaat atctcctttt agatatgtac caacatggtc agatacactt tcaaattatc
      781 ataatatgga aaaatatata agccttatgt gtaaaaagct agaagatttt tattatggtt
      841 ctgctataaa gagtgttgtt gattggtaca tagaatctct tggaagtgct accatggaaa
      901 acaatataat atctgtacaa attgcacttg aaactttgtc atatgtaatt ctagtggaac
      961 aaaataagat acttacagat gaagtatttg actgtaattt ggcatctaaa aatataagac
     1021 tcttacttga tacatgtaag ataccatatg gtaaacatga attaaatata tttgataata
     1081 taataaaaaa taaatttgat gatggagtgg atttagttat ttatttaaga aatagcatag
     1141 ttcatccaag tagaaagact catagagctg tgttagaggt tgaagatatt tggaatataa
     1201 tttcaatagg aacgagatat attgaacttg ttttactctt tattttaggt tatagaggag
     1261 aatattcaaa cagattagta gaaagatgtt atggtgaagt ggaagttgta ccttggaact
     1321 agatttttat atttatttta aaatatatat tgacatatat tgccttatgc tataaaatca
     1381 atatcataat aataattcag aataacatta aatgctaaga aaagaaatag taaaagtagt
     1441 taatcttaaa cagagagttt ccgattggtg agaggagcaa tagattttac tttgaacaca
     1501 tctttgagca ggactctgaa agagtagaaa tactttatta ggagatttcc ggaatccttg
     1561 cacccgttat agtgctagag tataataatt ttattgtact taaagaggtt agtgtcgtga
     1621 ggcattaatg aacagaggtg gaaccacgag agttaactcc cgtcctctag cagaagctag
     1681 aggcgggagt tttttaatat aattttttaa gcgcgcagag aattatattg caatagatac
     1741 attttttaaa aaataatcaa aatattttaa aaattaaaag acgagggaga atattatgaa
     1801 aaatatatta aaaaaagcag gaatatttac aattatgtta ggtttattag gtggagttgt
     1861 aggttgttca aaaccagata atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga
     1921 agtagttgta gggtttgata atacgtttgt accaatggga tttttagatg aaaagggaga
     1981 tacagtagga tttgacgtag acttagctaa agaaacattt aaaagacttg gaatggaagt
     2041 taagtttcag cctatagatt ggtcaatgaa ggaaactgaa ttaaatgatt caaaaactgt
     2101 agatgtatta tggaatggat attctataac agacgaaaga aagaaaatag tatcatatac
     2161 agaaccatat ttacaaaata agcagataat agttactcta agtgactcaa aaataaattc
     2221 aaaagctgat ttaaaagata aagaagttgg aacacaacaa ggttcaacag cacttgatgc
     2281 agtggaaaag gataaagatt ttatgaatag cttaaaaggt ggagcaccag ttctttatga
     2341 tacttatgat aaagcactta gagatttaga gataggtaga actagtgcag ttgttggtga
     2401 tgaagtttta attagatact atatgggaca aaagggtgaa gacaaataca aggtattaaa
     2461 agacgatttt ggattagaag attatgttgt agcaacttct aaagaaaatc cagaactttg
     2521 tgagaaaata aatgaaacat taaaagaaat gaaaaaagat ggaacttttg acaaaattta
     2581 tgataagtgg tttaaataga agataagtta gctgttaaga agggggcact aaaggcatgt
     2641 tacaaggttt agagatagtt atagcaatgt tttgtataac attgatagta tcaattccat
     2701 tggggattgg agttgcattt ttaagattat caaagaataa attagtaagt ggtattacac
     2761 aatgttatat attgataatg agaggtacac ctttactttt gcaaatgata gtcatatttt
     2821 acggattacc tcttttagga attgtatttg acagatttac agctggagta gtagctttct
     2881 tcttaaatta tgcagcatat tttgcagaaa tatttagagg tggaatacag tcaatcgata
     2941 gaggacaata tgaagcttca aaagtactag gatttgataa gtttactatg tataagagag
     3001 taatctttcc acaagttttt aaaagaatac tagcacctat ttcaaatgaa gtaatcacat
     3061 tagtaaaaga tacatcttta gtatatatat taggattaaa tgatatttta agaatatctc
     3121 aaatagcaat gaacagggaa gcaagtctgt taccattatt tgaggcagga gcgatatatc
     3181 ttatatttgt agctatactt actaagggat ttgaattact tgaaaaaaaa tattcttatt
     3241 atagataaag ttacaatatt ataggaggag gttttttata tgttgaaaat caaaaactta
     3301 aataaatcat ttaaaaaaaa tagagtactt aaagatattt cttttgagtt agaagaaggt
     3361 cagattggag ttttacttgg taagtctggt gcaggaaaga ccacaatatt aaggtgtata
     3421 aatggtcttg aagaatttga tagtggagag attataatag ataatgaggt aataaaaaat
     3481 aagagagata tggcaaaaat acgaggaaaa ataggtatgg tttttcaaaa tttcaactta
     3541 tttcctcata tgacagtcct tgaaaatata attgaatccc cagttaatgt ttttaaagtt
     3601 cctagaaaag aggctgaaga gagggctaga gaacttttaa gattagttga tttagaagat
     3661 aaattaaatt cttatccatt tgaattgtca ggaggacagc aacagagagt tgctatagct
     3721 aggtcatgtg cattgatgcc aaaagtcctt tgttttgatg aacctacttc tgcacttgat
     3781 attgatacta ttcaaagagt agtcaatata atgaacagac ttaaagacaa aggtatgaca
     3841 atattaataa ttacacatga tgttgtcttc tcaaataatg ttgcagataa aattataagt
     3901 ataaaagatg gtatagtaga aaatgttcaa ataaaagaaa aaattgttta gagttttata
     3961 aatataaact agggtagaat tttataatat aaataaaggg caagtaaaat caaatatact
     4021 atgccctttt gttatctcaa aatttaatct tatattttat gtttaagtta cattataatt
     4081 tataataaaa ataaaaggac tatttaaata tataatgtga ataaatgttt aataatttga
     4141 aatatgataa ttttttatgt tgaaagctta taatgattat gatataaatt ggttatagta
     4201 tacaatttat gggtataaca ataaaaaatt agaaaggaat ttttataaaa tgattaaatt
     4261 atatggatat acaaaatgct ctactgttaa aaaagctaaa aattggctta aggaaaataa
     4321 tttagaattt gaagatatag atatggttca aaaccctcct agtaaagagg agttaaaatc
     4381 aatttataag actagtggat atgatataaa gaaatttttt aatacaagtg gtatgaaata
     4441 tagagaattg ggtcttaaag atattgtaaa aactgaatct gatgataaac ttttagaaat
     4501 attggtaagt gatggtatgc ttattaaaag accattactt ttggatggta aaaatgtatt
     4561 attgggcttt aaagaagatg tatggaaaag tactttgcta aaagaagatt aggaagagat
     4621 aggtgtgttg attaaaatta ttaattaata cacctataaa tttatgtatt aaatctaatt
     4681 tttataaata ttaattagca aattcaaatc aaaaaaattc atatcaacaa acatattact
     4741 tcttattaca aattatatca tatgttttat attaaaatat tataatatta caacgaattg
     4801 ttacaaaatt acttattatt aaccatgaac atttgttttg tgctatcata tttatataag
     4861 ggaattgtca taaaaaatat cttgttcata cagttttata tagaaaaaag aaatgaatct
     4921 tttctttata taagta
//
LOCUS       DAELJO010000241         4927 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELJO010000241 DAELJO010000000
VERSION     DAELJO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.243"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            248..754
                     /locus_tag="KPB88_003512"
     CDS             248..754
                     /locus_tag="KPB88_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG0055749.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1167..1835
                     /locus_tag="KPB88_003513"
     CDS             1167..1835
                     /locus_tag="KPB88_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055750.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1994..2500
                     /locus_tag="KPB88_003514"
     CDS             1994..2500
                     /locus_tag="KPB88_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG0055751.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2628..3644
                     /locus_tag="KPB88_003515"
     CDS             2628..3644
                     /locus_tag="KPB88_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0055752.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3987..4691
                     /locus_tag="KPB88_003516"
     CDS             3987..4691
                     /locus_tag="KPB88_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG0055753.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         2085 a          419 c          817 g         1606 t
ORIGIN      
        1 ctacattttt ttattgtata ttactttagg tttaaaaata tattatatac tttataatta
       61 atatatttta tctatatttt acacttataa aatatgaaaa ctacttattg cgaaagtaat
      121 tgatttgttg tattataatt tacatgaatt atgtagggca atcaaaagtc agtattataa
      181 gagtacatat attcaaattt ttaacaatta atttatataa ttaaaaaatg aagggtagga
      241 tgaagtcatg ggttttagag aagtaaaaat tgaggaatta cagtttaatc catttactaa
      301 gattggcaaa gaatggttac ttataacagc tggtgattca gaaaagttta acacaatgac
      361 tgctagctgg ggtggtgttg gagtctattg gggtaaaaat gttgttacaa cttacataag
      421 accacagaga tatacaaaag aatttgtaga tagtaatgat acatttacag ttgccttctt
      481 tgatgaaacg tatagagaag ctttaaatat ttgtggtact atttctggta gagatataaa
      541 taaaatagaa aaagcaggac ttacaccata ttttgtggat gatacagtag cttttgaaga
      601 agctaatatg attatagtat gtaaaaagct atatcatggt aatatgccac cagaaaactt
      661 tgatgctaaa gaaaatgata aaaaatggta tccagagaaa gactaccata ctatgtacat
      721 ttcagaaatt ataaaagttc ttgtaaaaga ataattataa taaaaaatat atgaaaagaa
      781 cttattataa gtaaatgact gtgctttctt atctgatatc taagtagatg ctagtaacta
      841 aataaatatt aattttaaat aaatactatt ataaaaaaga tgaactatat ttactatagt
      901 tcatcttttt atttatataa gcacttatat aagtagagat ttatttgaat aaaagtattt
      961 atttgaaata cttgagtata ctttataagt atttttatgc taactagaaa cacttaattt
     1021 atggcatttg tacttgattt attttaatgt aaataatttc aaagttaatt ttattcaata
     1081 tagtactata aaagtataaa taacatttaa taaaaatata tattatatta ggtattttaa
     1141 ataaattttt taaagggtga ttttttatgg aaaatataac tagttatttt acaacaatac
     1201 tatgtgtatt tatatgctta tcatctgtat ttatatttac tcaactagct agagtattta
     1261 ttaataagaa gaagattaat caaaaaatta aatcaagaaa cggatttagg tatgatagag
     1321 attttattga agccagaaga gaagaaatac atataaagga taacaataat aataaaaata
     1381 agagtaacaa taagaaatta aaggaagaaa aagtatttaa atacgataat ggtgatttat
     1441 ataaaggtga gtttgttgat ggaaagaaaa atggatttgg aatatatatt ttttcaagta
     1501 aggaaaagta tgaaggatta tggaaagatg acaagatgca tggaatagga aaatatactt
     1561 atagagatgg ttcaatatat acaggtgaat ttaaatacgg attaaaaaat ggtttgggaa
     1621 aattaacata tcccaataat gacatttata agggatattt tttagataat aagaaaaatg
     1681 gtaagggtgt attatataaa aatgatggaa ataaacaagc aggaatatgg gaagatgatg
     1741 aacaatgtaa aagtttagat tttaaagatt taaataataa taaaataaat aatgtttatc
     1801 aaaatagatt aaataacaat aaattaaaag tttaattatt ctaataaaaa aatctttatt
     1861 tatgatggaa tataggatat atctatgttg taatttgtaa aatcttccta tatcatataa
     1921 aattaagtat tgattagtct attacataaa tatagaatat aaatatatcc atttaataat
     1981 aaggagaaga gatatgaaag agtatgtagt tgtggattta gaaactacag gattagatcc
     2041 atataaaggt tgtgaaataa tagaaatagg aataacagaa ataaaaaatg aacaaattgt
     2101 aaaaaactat tcaaggttga taaaacctaa ggggataatt tcttcattta taacagaatt
     2161 gacacatata agtaatgaaa tggtagaaaa tgaagaacca ctagaattag ttttacctag
     2221 atttagaaaa tacataggtg atagaactat aattgcacac aatgcgaaat ttgatttaaa
     2281 atttttaaat tactatctta gaatgttaga tttagaacca ataaacaatt atatatgtac
     2341 agtagagtta ttaaaaaaat gtaaaagtta taaaggaaaa aacaagaaac ttgaaacagc
     2401 ttgtgcttat tacaacatag agaatataaa tgctcataga gctgacagtg atacattagc
     2461 aactgcaaaa ctatttttga aaataaaaga tgaatattaa ttataaatat agagaaaatt
     2521 agtgaaaata tcctttataa aattgaccct taagcttaca attttgttct ttgatttatt
     2581 ttgtagaaca atgtataatg taccttatat gaagaaggga gactaaaatg ggttattaca
     2641 atgataatga taataataaa ataagacgta aaaaaattag taacaacaaa gatagtaata
     2701 aaaaatctaa acattcttat acttcggcaa ataaaaaaaa taaaagtcct aatacaagtt
     2761 ccagtaaaag aatgagtgaa aaagtagatt cacagaatct tataaaaaga gatagagata
     2821 gttttacaag agataatgta agacctttaa atacagatta caatcaaaga gaaagaagaa
     2881 gagtcgagta taaacaaaga gagaaaaaat taaaaagtaa aaaaagaaaa aaacaaaaaa
     2941 ggattagaat agttagggca ttattatcta ttatgttagt tgcactaatt atattttcag
     3001 tggcaggtgc aatatttaca gtttcagcta taaaagggtc accagaagtt actaaaaagt
     3061 taattaggtc taaatatata agtagtgagg ttgtaagtat aaaacaaatg ccaaatgatt
     3121 taaaacatgc aatagtttcc atagaagatg agagatttta taagcataaa ggagtagatg
     3181 ttatttctct agttaggtca gtattaaaca atgtgtttac agatacaaca caaggtgcaa
     3241 gtactataga tatgcaagtt tcaaaaaacc tgcttactag taatgataaa actatgaagc
     3301 ggaaaataag ggatatgtat aatgctaagc agatgaataa gattatgact aaggatgaaa
     3361 ttcttgaagc atatttaaat aatatgtatc taggtaaaaa tgcatatgga gctgcaaagg
     3421 gagctgaagt ctacttcggt aaaaaagtta gtgaattaaa tttagctgag tgtgctatgt
     3481 tagctggtat gacaaataat cctgcaaggt atattgacca tggagaggct aagaagagac
     3541 aagtgattgt actgtataag atgcatcagc ttggttatat aactgataag gagtatagaa
     3601 tagcaaaagc tgatgacact ccatttaaat cagaaattga ttaaaattgt aagaatatag
     3661 tattttcaat agtttaaaca aacaaaaatg taataaaaaa ggtgatttgc caccataatt
     3721 ataatctaat ggcaaattgt tttaatgtac aaactagata atagaatatt gttttaaatg
     3781 ctatattatg ctaaaattta attgtatttt gtaaattaaa aaacttataa tttagttttc
     3841 tagggttccg cattttataa tggtcaggtc caagagaaaa cgcgcaattg ttgtgtacac
     3901 ggaaggataa aagcctggga gatatttaag aatatttcta taggcttttt tatttttaca
     3961 tattttaaat ttgaggaggt tttctaatga aaaaatttgt tattgaggat gcattttgga
     4021 atctatttcc tagtgcaaaa attggagttg ttatttgtaa tgatatagat aactctataa
     4081 aagatgaaga ctataagaaa ataattttga agggagaaga agaagcatta aaatacttgg
     4141 aggactcaga atttagcaac aaccaagtga ttaaagtatg gagagaagca tttaaaaaat
     4201 ttaaaacgaa aaaaggagca aggtcttcta ttgaagcatt attaaaaaga gttcataatg
     4261 gaaataatat aggaactatt aaccctttag tggatatcta caattttatc tcattaaaat
     4321 atgcattacc ttgtggaggg gaggatatag ataaatttat aggagatatt aggctaacta
     4381 gagcaattgg taatgaggag tttatcccac tagggacaga tgaaaattca tttccatatg
     4441 aaggagaaat aatttataaa gatgatggtg gagcaatttg tagatgttgg aattggcgtg
     4501 aagcagtgag aacaatgctt actgaagata ctaacaatgc ctttttatgc attgaattag
     4561 tagatgaaag tagatttaaa gaatttgaaa atgctttaaa tgaattagct aaaatagttc
     4621 aagaaaaatt gggtgggaat tgtaaaattt caattttaga tattgataat aaagaggtat
     4681 cagtggatta atatggaggt gctgttgtaa tggactattc ttagttataa agatacttat
     4741 atttagggta agaaggtcta ttttagatat aacgattatc aaaggatata atatagaaaa
     4801 taaaaatgta gaatataaaa agcctttaaa ataagaaaaa aatgtatttt aaaggctttt
     4861 attatacttt ttttatttgc ataattaaca ataaaaatat attgttaatt ataatatttt
     4921 tattgaa
//
LOCUS       DAELJO010000242         4887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELJO010000242 DAELJO010000000
VERSION     DAELJO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.244"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            42..845
                     /locus_tag="KPB88_003517"
     CDS             42..845
                     /locus_tag="KPB88_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055754.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYANNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            857..1987
                     /locus_tag="KPB88_003518"
     CDS             857..1987
                     /locus_tag="KPB88_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0055755.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSKPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1991..3400
                     /locus_tag="KPB88_003519"
     CDS             1991..3400
                     /locus_tag="KPB88_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0055756.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSRKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3611..4120
                     /locus_tag="KPB88_003520"
     CDS             3611..4120
                     /locus_tag="KPB88_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0055757.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2030 a          414 c          856 g         1587 t
ORIGIN      
        1 taatatagat ataaaaataa ttatagaaag aaaggcacat tatggaaaaa ttgagagtta
       61 ttgaaatgat aaaaaataat cccaatatta tagctacaat agataatcca actgatgaaa
      121 tgaaattgtt agctattaaa gaaaatgggc ttgtactaga atatataaat aatccaacca
      181 gagagatgca agaactagca atagataata atattagagc aattaaattt atagataatc
      241 caacagaaga tatgatgata aaagcagtaa atgagggatg gagtatactg gattatatca
      301 agaacccaac tgataaagtt attgaaatgg ctataaatca agcaggatgg gcaattaaat
      361 atgctaacaa tccaagtgaa gaattacaac tattagcagt aagaaaaaat tatgattcga
      421 taaagtttat aaaacaacct tatgaaagtg tacaagagga ggccgtgaga ataagttatg
      481 atgccttgag atatatagat tcacctagtt ataatgcaga acttatagct ataaaaaata
      541 atgaagcagc tattagcttt ataactgatt tagataaaaa taaaatgttg gaatttttaa
      601 aagtgaatat tttagtgatt aagtacattc tgaaaaaagt atctaaagat gaattagaga
      661 gtgtattaaa agaagtatta tccaaagaag atgttgagga aaagtatatt agagattttt
      721 tgaattgtag tgttatagat agaaatagcg aaagtatacc aatagataaa attatgttta
      781 tttataaata tggaagtaag acagcaaaaa aaatagctgt agatgagaaa ttaaagatta
      841 tgtaggagag agaaaaatga attttataga cacattaaaa agcgttgact taatattatc
      901 cactggagaa gtacctttaa tagtaggtga aagtggaata ggaaaaacag ctttagcaaa
      961 taagcttgcc aaagaaaatg attggagttt aattgttatt gatggaaact tacttaaaga
     1021 gggtgaaata ggtggtcttc caactataga atcttatgta ggagttaatt ctaatggata
     1081 taagactgaa aagaaaacaa cagtgtatgc tgttcataac aaattaagag aaattgatga
     1141 agagatatct aaaagtaaga cagttctttt atttatagat gagataaatc gttgtgagca
     1201 tacagtacag caagaactta tgaatttaat tttaaataga gaaattaatg gatataaact
     1261 gcatgatgat gtaaagattt tagcagcaat gaatccatca agtaaatatg gttcagattt
     1321 tgattatcaa gtggtagata tggatgcagc ccaagaaaat aggtttgttt ggttaaatat
     1381 ggagagtgac catacacaat ggataaaatg ggcaatagat gagggtattg aaagaaaagt
     1441 tatcgagttt atttccactt ttccagaata tctgcataaa ataaatgaag atgatgtaag
     1501 agccactcct agaagttatg agagagtatc taaaatttat aaagtttata aagaaaaaaa
     1561 taattctatt cctagagcag tatttttaaa tgttgtaaag ggtaatgtag gtaaagttat
     1621 tgcagaagaa tttataagtt ttattgaatc aaactctaaa ccattaatat cttatgaaga
     1681 tgttttttta ggagaatcta ttgatgagtc cattgtagaa agagtaaaaa atgagagtca
     1741 tacaagactt tatctatcag ctatgaacat tctaaaagac ttagaattaa atattaagaa
     1801 tgataaatat gaatcaaacc actatattaa tagatttatt gaatttttaa aaatgtatcc
     1861 tgtagattta atgataggta ttatgaagga tattagaaac agttatattg aagtatataa
     1921 aaaagctata gaaaatgaag agtttgtaaa atcatatttt gaatcttata gtttaataag
     1981 gggataatgt atggaaaatt attttgataa acaagcaaaa gagctttata ataaagcaag
     2041 tgaaattata aatactcatt ctatgctaaa ggcaaatcgt gctaatgaga aatttgatat
     2101 agatatacca caagatttta agtatgagtt ttttagtctt gtagataaag tgaatttaag
     2161 tcttatggaa gaagaagata atttttatgg atattttttg tttcagatgt caagagaaat
     2221 aagatttgat ataagtagtc ctacaggtgt gaattttaag ggagctaaat atgttatata
     2281 ttttaatcca ataatctttt taacgcttga cataaagcaa atggagacta caattaaaca
     2341 tgaaattcat catatattat ctatgcattt gatgagagca aaggagttaa aaggaaagta
     2401 tagtacatta gcaattaata tggcaatgga tatagtagta aataaatttc taaacaattt
     2461 accaccatat gcaactacct tagagtgggt aaatttaaag tattccttaa aacttgaacc
     2521 atatgaacct tttgaatatt atgtagaaaa gattcagact gaattagatt tgatggaagt
     2581 ggatgaagat ggtgaggaag atgatagtga taaatatgga gatatagaaa ttgattatag
     2641 ccccgaaaga actcatgata tatgggatga atccaatgag atagatgaaa atacccttag
     2701 agaatttact gaaaaattta ttaacagttc tcgaaaaggt aaagttcctg cttatttaga
     2761 aagtatgata tcatcactta aaaatagtaa gggtgaattg ccatggaatt tatatcttaa
     2821 tagaataatg ggaacagttg aaaataataa aaagaagact ataacaagaa gaaatagaag
     2881 acaaccaaat cgtttagatt tgaggggaga acttagaagt cataaggcag aaatagcagt
     2941 tgcacttgat gtaagtggaa gtattagtga tgaagagttt aaacaagcta ttaaagaagt
     3001 tcttagtata gtgaaaaact ataatcatga aattacaatt atagaatgtg ataatgaaat
     3061 tagacgtgta tataaagtga gaactgaaaa agatataaaa gatagaatta ggataggtgg
     3121 aggtactaga ttttctccag tctttgagta tgctaataat aaaaagatta atttgttagt
     3181 ctactttact gatggtaaag gtgaaaataa actacaagta atacctagag ggtataaaat
     3241 tttatgggtt atttctggaa gaggagataa aatttcatta aaagaacctt atggagcagt
     3301 taaaaaactt actaaggtta aaacaaatga tgaaatatta gaaataaatg atgtaagaaa
     3361 tgatgggtac tcaatgaata gccaagcacc aattttatag gtattcacag gagataagct
     3421 gtattaattt attttgatat gcttgttata aaaatctaaa aaatacatta atcaatggtt
     3481 aatgtatttt ttaattttaa taatataagt tttacttaca tttaagtctt aaaatgtgtt
     3541 aaaatataat atatagcaga tagttttggc aaggtggatt tagttgtcaa aactattttt
     3601 tttattaagg atggtgatgt ttttgtttaa gattgatgat tatataatgt atggtatgac
     3661 aggagtctgc aaagtcatgg atataacaaa tgaacgattt acaaatggaa taaaaaaaga
     3721 gtattatgta ttgagtccta tttattctaa taatacagta ataaaaattc ctgtagataa
     3781 tgaaaaagta ccaatgagaa agttactttc taaaattaat gtgctttcat taataaatga
     3841 tattcctaat atggatacat catggataga taatgaaaaa ttgagaagtg agcaatttaa
     3901 aaaaatactc agaggtggta aatgtgaaga gttaataaaa ttagtaagaa gtatagataa
     3961 taacagagag tatgtaaaat ctattggtaa aaaaacacat caagcagatg ataatataat
     4021 gaaagaagct gaaaggttat taaatgaaga atttgcaact attttagata tttctcctaa
     4081 tgaagttagt tcatatattt caagtcatat acctcaataa agtgttttag tatcctttat
     4141 ttaatctatt ttaagtatta tttatgtatg ctggttttgg taaaataaaa tatataatta
     4201 aattatataa aaaataataa tgattgtatt gaagaattac agaatagtgc ggagagccta
     4261 ctcctttagg tgtgagatgg atatcactta aagatttaaa ttgttattgt agatgttttt
     4321 aatataaaac aagattaata agtgtattgt aagctttaag tgaaattgat actataatga
     4381 gttacttaga ttttaaaata tataatatta taaaaaatcc ttttaggttt tgaatgaata
     4441 agatggatac tatgttgaat agaatccatc ttatttaagt tttatttatg ttgtttattt
     4501 gtaagcatta tttttgtaca tgaaaacaca gtgtaaaaat atgcttagaa ttatgaagtt
     4561 ttattaaaaa gatattcgac aaaataagac taaaaatatt cttaaactta taactaagat
     4621 aaatattgta tatttatagt gtagcctgaa aaattgtgga tgtacgaaat ttatttagta
     4681 ttttattgaa acaaagtaga agaagtacta tagtttatct ttgaatagat tgttattttt
     4741 tagtgaaaaa gttattaggt taatacataa atataatagc ttataaatag tttaagcatg
     4801 ttaaaaaagt agttattaga gatatatagt tacattgctt ataagatact taaaatgata
     4861 gcattcactt atatttagaa aattaag
//
LOCUS       DAELJO010000243         4887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELJO010000243 DAELJO010000000
VERSION     DAELJO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.245"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            467..976
                     /locus_tag="KPB88_003521"
     CDS             467..976
                     /locus_tag="KPB88_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG0055758.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1004..1198
                     /gene="rpmI"
                     /locus_tag="KPB88_003522"
     CDS             1004..1198
                     /gene="rpmI"
                     /locus_tag="KPB88_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG0055759.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1254..1610
                     /gene="rplT"
                     /locus_tag="KPB88_003523"
     CDS             1254..1610
                     /gene="rplT"
                     /locus_tag="KPB88_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG0055760.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1920..2465
                     /locus_tag="KPB88_003524"
     CDS             1920..2465
                     /locus_tag="KPB88_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055761.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2467..2558
                     /locus_tag="KPB88_003525"
                     /pseudo
     CDS             2467..2558
                     /locus_tag="KPB88_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
     gene            2900..4825
                     /locus_tag="KPB88_003526"
     CDS             2900..4825
                     /locus_tag="KPB88_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0055762.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
BASE COUNT         2054 a          484 c          929 g         1420 t
ORIGIN      
        1 tagtatatta gagtacaata tagaatttgt tatgtagtat agtagtatat tagaatacaa
       61 tatagaattt gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta
      121 gaatatatag agtcatgtag aatatacata tataaaagga ttttgggtat aaatttaaaa
      181 attaatagca aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt
      241 cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaaa taaggtaaga
      301 ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa
      361 gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt
      421 acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt
      481 agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca
      541 acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt
      601 ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt
      661 tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt
      721 aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa
      781 tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag
      841 agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa
      901 agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat
      961 agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac
     1021 tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa
     1081 agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa
     1141 atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga
     1201 tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa
     1261 gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag
     1321 gattcagagg atctagaagc aaattatata gaccagcaaa tacattcgta atgaaagcat
     1381 taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac
     1441 aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat
     1501 taaaattatc tggtgttgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc
     1561 cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca
     1621 tgagatgtaa agtctttgag ttatgtaaat aattcaagga cttttttatt ttttgaaaaa
     1681 tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata
     1741 gaattttatg agatttatta taagaattgt tacaattatt tatgatagct ataaaaagtc
     1801 atagtaaaaa tgatattgat gtatgttgat atatgtataa agctttcaat caatcttgaa
     1861 ttaatattat caacatgata atattatctt attaataata taaataaggg ggagataata
     1921 tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa
     1981 ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa
     2041 aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtagttg
     2101 aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg
     2161 atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc
     2221 aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata
     2281 tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag
     2341 ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac tcacatgaag
     2401 aaggtagaag aatgcattct ataggagtga taattactcc acctaaagag gatgaaggga
     2461 aataaatctg atttacatgg ggtagagcca atttatacag ggaatcgttt tatgatttac
     2521 atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtacc atttcatcca
     2581 atgacttgga gtggtacaat gaagtttttt aatttttcat attaatgcta aagaaatgaa
     2641 ctttagacca catatgcaat taggttgcat tatacaatta gaccacatat acaaaataaa
     2701 aatcagtatt tttatagcag ataaattgac tgttattctt cttaaaaatc atctattagt
     2761 tattgttagc atattgaaag aaattatagt aatctattga ataaatgaat attgcccata
     2821 atcatataaa ctgctatagt ttaaaataaa gcaggacgtt aaattttata gtaatctaaa
     2881 aatttaagag ggggatttta tgaaaataaa atttgttatt atgccattgg tgatattttt
     2941 aatggtatta tttgtggggt gtcaaagtac aaaagataca gaagttaaag caaaagatac
     3001 agaaattaaa atattaggta caggtgattt gcattcaatt ttgacagact ctatggtttc
     3061 gtatgtaaat gaggaaaggg aaaagaataa aaatctgctt atggtagatg cgggagattt
     3121 ctatggcaca caaagtagag aaatgtggga atggtcttct ggaaaaaagt taattaatat
     3181 taaaagaaat ggtagagcag agtatgatga cataattaag agtagtaaag atgaagtacc
     3241 tatagttaaa gatatggcaa agttgaaata tgatgctgta gttttgggag ataatgaatt
     3301 tgtttcaaat gataagcaaa gtcttgataa actagtttct gattttaaaa ataataatat
     3361 gccattggta tcagcaaata tatatgaaca atctggagaa aattatgtac aaccatatgt
     3421 aatgaaaaat attaagacag atgagggcaa tgtaaaagtt ggaatactag gtcttactat
     3481 aaaagaagta ggggaaaccc ttggtcttga ggattttgaa aaggataaaa aagctagaga
     3541 gcttggagaa caagctgatt acaaaggaaa attatatgca aatgatttag tagaagatgc
     3601 aaaaaaatgg ataaaagtta tggaaaaaga aagtccagac ataatagttg cagttgtaca
     3661 ctcaggggaa gaacctaaaa cagatagaaa tccaggaaat agaataaaag agttagcaac
     3721 tacagtggat ggaatagatg caattgtagc tggtcatact catgaaaaaa tagatgaaca
     3781 caaatataaa aataaatctg gagatgaagt aattgttaca caaccaggtt ggcatggaga
     3841 tagagtatca gagattaatt tcaaattaaa taaagagaat ggaaagtggg acataaagga
     3901 aaagtctagt aaattaaaaa taattgataa agaagtaagc atagttgcta ctgcagattt
     3961 acattcacat ttctctgata aattaacagt tgaccttgtt aatgaacgta gtaacccaat
     4021 tgaacctgtt gtagtagatg caggcgactt cttagaccct caaacagatg aaatgactca
     4081 atggtataaa gagtggaaaa gtataagaga taataatagt gataaaacga ttagtagatg
     4141 cccaatggtc atggatatga atcagggaat gtatgatgca gtagtacttg gaaatcatga
     4201 atttgtatct gaaaaagatg aattttggtc agatgaacgc cttttagatg ctgttataga
     4261 ggattttgaa caggtggcaa tttctgtgtt atcggcaaat atatataggc aatctggtaa
     4321 aaattatgtt aaaccatata ttataaaaga tgtggagacg agtgaaggta atgtaaaagt
     4381 aggaatattg ggacttacta taaaagaagt aggtaaaggt cttaaaaatg taaacttaca
     4441 agagcaattt caatataagg ataaattgta tgcaaatgat ttagtggaag atgcaaagaa
     4501 atgggtaaaa gttatgaaaa agaaaaaacc agatataata gttgcagttg tacattcagg
     4561 agaagaacca aaaaatccaa aacatccagg aaatagaata aaagaactag caactacagt
     4621 agatggtatt gatgcaattg ttgctagtca tactcatgaa aaaatagatg agcatgaata
     4681 taaaaataag tctggagaaa aagttatagt gacacaacca ggtgagcatg gtaaatatta
     4741 ttctaaaatt acctttaaag taaataaaga aaagggttcg tggactatta atgataaatt
     4801 cagtaaaaca attgaggttg aataacttta taaaatttat agataaagta aattatctga
     4861 tattgacatg atatataaga taaagtt
//
LOCUS       DAELJO010000244         4870 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELJO010000244 DAELJO010000000
VERSION     DAELJO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.246"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1568
                     /locus_tag="KPB88_003527"
     CDS             <1..1568
                     /locus_tag="KPB88_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055763.1"
                     /translation="VNLNSNLYDDVDRVEYIIRKILTLSNKEKNTIKLEDLADSMYVS
                     VSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVDLSNKRDNQLSLND
                     FLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLSRHKYNEKEFIENY
                     IENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKVFEEFLYKHISSLC
                     KKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINEDEVANIALHIGGA
                     VERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILKIIPSYLVEYIKVI
                     DVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLDLDIKSLFDSELFF
                     TDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIGNMVTIPHGAIGKI
                     FDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIYKRVDSIAKVISIC
                     ENSSFEKFVNLFN"
     gene            1735..2652
                     /gene="pfkB"
                     /locus_tag="KPB88_003528"
     CDS             1735..2652
                     /gene="pfkB"
                     /locus_tag="KPB88_003528"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0055764.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            2689..4608
                     /locus_tag="KPB88_003529"
     CDS             2689..4608
                     /locus_tag="KPB88_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0055765.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1972 a          469 c          897 g         1532 t
ORIGIN      
        1 aggtaaatct aaatagcaat ctctatgatg atgtagatag agtagaatat ataatcagaa
       61 aaatattaac cttaagtaat aaagaaaaaa atactataaa acttgaagat ttagccgatt
      121 ctatgtatgt aagtgtttca acagtaaaaa atgatttaaa agaagtaaaa agaatgttgg
      181 aaaagtattc aatatcagtt aaatctaagc ataaacaagg tatatgtgtt ttggaaagtg
      241 aagataagat attaaattgt atagttgact taagcaataa gagagataat caattgagtt
      301 taaatgattt tttaaatgat gatataaagg aaagaaaact attaataaaa aaattattat
      361 tagacatatt aaatgaaaat ggattagtat taactgatat agaatttaag agtgtgttaa
      421 ataatatatt agttatgtta agcagacata agtataatga aaaagaattt atagagaatt
      481 atatagaaaa ttataggagc aaaagaagct caattttaga aaatgaacaa aacaaagaaa
      541 ttataataaa ttcaataaaa acattttgta aaaatttaaa attagctaca tcaatagata
      601 taagtaatga taaggtattt gaagagtttt tatataagca tattagtagt ttatgtaaaa
      661 aaataaagtt aggtataaat caatcaccta taatggctca agatataaaa ataaaatatc
      721 catttgcttt tgaactagct aatatagcta aaaagacaat agaaaaagat ttgaagatag
      781 aaattaatga agatgaagtt gcaaacatag ctctacatat aggaggagca gttgaaagag
      841 catcttataa taataaagat aaggttttca aaactataat agtttgtact tcaggcatag
      901 gcacatcaat gttaataaaa gctaaattag agaatatatt taaggaaaaa ttagaaatac
      961 taaagattat tccttcatac ttagttgaat atataaaagt tatagatgtt gattttgtaa
     1021 tatctactgt tcctattgaa gtaggagatg tgccagtaat aaatatatct ccaattttaa
     1081 gtgagaaaga aattagatta attgaaaaat atatagaaac aggaaatata tacctagatt
     1141 tagatataaa aagcctgttt gatagtgaat tgttttttac tgatttggtg tttaaaagga
     1201 aagaagaagt tatagatttt atggcaagta aacttgtaca aaagggctat atagatgagg
     1261 atatgaaaaa atcttacttt gagagagaaa aaatagcaac aacagaaata gggaatatgg
     1321 taactatacc acatggagct attggaaaaa tttttgataa taaaatagca attggtatat
     1381 taaagaagcc tatttcttgg gaaatatcag atgttcgttt aataataatg ttagcccttg
     1441 ataaagataa aatattagat tatgaagtaa tattttcaaa aatatataaa agagtagatt
     1501 ctatagcaaa agttataagt atatgtgaaa atagtagttt tgaaaaattt gtaaatttat
     1561 ttaattagat gggtagtatt gtatatacac ctgtaaattt ggataatata aaactaaata
     1621 agataaaaac gataaatttc tcttttaatt aagagaaatt ttttgacttt caaggatatt
     1681 aatataaagt taagatataa acataaagca aataaataaa aaagaggtgg caatatgatt
     1741 tatacagtga cattaaatcc atcaatagat tatatagtta agctagatga gctaaagaca
     1801 ggaagtacaa atcgtgtaaa agaagaatac gtttatccag gaggaaaagg tataaatgta
     1861 tctcgcatac ttaaggaact tggaaatgat aatacatctt taggttttat aagtggtttt
     1921 actggagaat atataataag aacattggaa gaaaaagaat tgaagacaga ttttataaag
     1981 ataaaaagtg gtttttcaag aataaatgta aaaataaagg cgttaaagga aactgagatt
     2041 aatggacaag gtccaaatat agatgatgaa gacattgata ctttatataa aaaacttgat
     2101 aagctaaatc aagatgatat tttaatattg gcaggaagta taccatctac tttagatgaa
     2161 aaactatacg aaaatataat ggctagatta gaaaaaaaga atataaaagt tgtagtagat
     2221 gcaactaaaa atcttcttct taatgtttta aaatacaaac cttttctaat taagcctaat
     2281 aacgacgaat tagaagaact atttggtgtt aaattaaatt caattgaaga catggtaaaa
     2341 tatgctagaa ggttaaagga aatgggagct ataaatgtct tggtatcaat gggaaaagat
     2401 ggagcattgt tgattacaga ggaagatgaa gtgcttataa gtgatgttcc aaaaggaaaa
     2461 gtaaaaaatt ctgtaggagc aggtgattca atggtagcag gatttatatc tgggtatttg
     2521 aatactggga aatatgatta tgctcttaaa ttgggagcag cttcaggtag tgcaactgct
     2581 ttttcatatg acttagccaa aagagaatac attgataaat tggtaaatga aatatctgta
     2641 aagaagtttt aaaatattaa aaaatatata gaaaggtaat taatatttat gaaaattata
     2701 gatttattag atgaaaagtc aattaagtta aatttaaatt caaaaaccaa atcagaagca
     2761 atagaagaat tagtagattt ggtcgcaaat agtggaaatt taaatgataa agaaaactac
     2821 aaaaaagcaa ttttggctcg tgaagaaatg agtacaacag gaattggaga aggtgtagct
     2881 atacctcatg ctaaaaattc ttcagttact aaagcttgta tagcagcagc agtgtcaaaa
     2941 gaaggtatag attatgaatc gtttgacgga agtttatcaa atctattttt tatgatagct
     3001 gcaccagacg gagctaacaa tactcatctt gaagtattat caagattatc tacaatactt
     3061 atggatgaag actttagaaa taaacttata aattcaagct ctgaaaaaga atttaaggaa
     3121 ataatagata aaaaggaaag agaaaaattc agtgaagaat atcaagatga gaaagtggct
     3181 gaaaaaaata taatagatac taaagagaat gatgctaata agtatcctaa ggttttagca
     3241 gtaacagcat gtccaactgg aatagcacat acatttatgg cagctgaaag tttaaataaa
     3301 atggcagaaa acaaaggtgt atcaataaaa gtagaaacta atggttcagc aggtgttaaa
     3361 aataagctta caaaagatga aatagaaaat gcaacatgta taatagtggc agcagataaa
     3421 aatgttgaga tggctagatt taatggcaaa aaagtcataa aaactaaggt ggctgatggt
     3481 atacacaaag cggaagaact tatagacaag gctgtaaatg gagatgctcc aatttaccat
     3541 ggaggagatg gaagtcacaa cgaatcaaat gaagaaagtg aaagtggatt tagaaaagta
     3601 tacaaacact taatgaatgg tgtatctaat atgttaccat ttgttattgg tggagggata
     3661 cttatagcga tagccttttt acttgatgat tatactatta acccttcaaa ctttggttca
     3721 aatacaccta tagcagcatt ttttaaagga ataggagata aagcttttgg atttatgtta
     3781 cctgtacttg ctggatatat agcatactct atatctgata gaccagcgtt tgttgtagga
     3841 tttgtaggag gtgctctagc aggtgatggt ggttcaggct ttttaggtgc actacttgca
     3901 ggttttatag caggttattt agtagaaggg cttaagaaaa tatttagtgt actaccagct
     3961 tcattggaag gaataaaacc agtattgtta tatcctttac ttggaacact tttgatggga
     4021 attataatga catttttagt aatacctcca gttacagcaa ttaataatgc aatggttgga
     4081 tttttaaatg gtttaggtgg aacttcaaaa atagttttag gattagtatt aggtggaatg
     4141 atggctgtgg atatgggtgg acctgtaaat aaagcagcat atgtatttgg agttgcatca
     4201 cttgaatcag ggcagtttga aataatggca gcagtaatgg ctggtggtat ggttccgcca
     4261 cttgctatag ctcttgcaac tacatttttt aagaatagat ttactaaaga agaaagagat
     4321 tctggtaaag ttaattatgt aatgggatta tcatttgtta ctgaaggagc tatacctttt
     4381 gcagctggag acccacttca tgttatacca gcttgtgttg gaggttcagc agtggcagga
     4441 gcacttagta tgttgttcaa cgcagctctt cgtgcacctc atggaggagt atttgttata
     4501 ccagtggtta cccatccatt tgcatatata ttagctatag ctgtaggtgc tttggttgga
     4561 atgatgcttt tagcattact taaaaaacca ttaaatcaag aagtatagca attaaaggat
     4621 attcaagaac ttaaaatatg atgttttata aaaattaata tattgctgaa ttattttaaa
     4681 ataaggattt tatgaggctg ttatataacg atttgtaatt gttatcttga cagtctttta
     4741 tttttatata agtaaaataa gtgtgttttg ctttatttgt atttaaattg tttttaagtt
     4801 agaatataaa gaaaattata tattatttta atattcactc gaactagaaa ataatatttg
     4861 ttgtattttt
//
LOCUS       DAELJO010000245         4840 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELJO010000245 DAELJO010000000
VERSION     DAELJO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.247"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      353..457
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            667..1758
                     /gene="gcvT"
                     /locus_tag="KPB88_003530"
     CDS             667..1758
                     /gene="gcvT"
                     /locus_tag="KPB88_003530"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBG0055766.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKK"
     gene            1837..3141
                     /gene="gcvPA"
                     /locus_tag="KPB88_003531"
     CDS             1837..3141
                     /gene="gcvPA"
                     /locus_tag="KPB88_003531"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG0055767.1"
                     /translation="MLKVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKR
                     LSEENLSLEDLTCFLGAGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFE
                     FQSMIAEITDMEIANASMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTY
                     LQYKDCEIVEIDFCNEYGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITH
                     ENKAMLIMSVDPISLGVLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTR
                     KMPGRIVGQSLDVEGKIAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMG
                     KEGFKEVGMQSMKKAHYTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEE
                     NILGGYNLEYNYPELKNSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            3141..4598
                     /gene="gcvPB"
                     /locus_tag="KPB88_003532"
     CDS             3141..4598
                     /gene="gcvPB"
                     /locus_tag="KPB88_003532"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG0055768.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRDDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         1946 a          526 c          886 g         1482 t
ORIGIN      
        1 caaattctat aaaaactaaa taatttcaag tatattttaa aaatcttatt tttcacaagt
       61 ttaaaattat aaacgatttc atcaagtcta tgaatatact tttaacatgt tatagtaaat
      121 ctcaatataa atttacattt ggttctttgt gtgttatttt aataataaat taagtgaaaa
      181 tgttatttta cattaatata taatatcttt gcataagagt gaataatata aaagagaaat
      241 aattgataat aaaaacttaa caaaaaaatt aatacataaa aaaaaataaa tataatgtgt
      301 tgacatttaa ttgaaatatt catacaataa aattgaaaat aaatatttcc aaatgagatt
      361 agcgagagag tcctttatac gaaaaggcgc cgaagaagaa atttaagaaa tgccaatttt
      421 tagagaagct ttcaggcaaa aatatcgcta attgatggga ctctggagag acttaagtta
      481 agatactata agtcaccgaa gaagcaaaat aatattaaat tattaaatct ttcaggtata
      541 caaacaggga tatataggct aggattattt tccatgctga tatatctttt ttttatgaaa
      601 aagtacagga aataatatca aagttactta aatacagtag gtctaatttt ctagggaggg
      661 ttatatatga atgaattgaa aagagtatct ttgtacaata tacacaaaga gcttggagca
      721 aaacttgttg aatttgctgg ttgggaaatg cctttagaat atgagggtat aaataaagag
      781 catgagaagg tcagaaaaag tgcaggtata tttgatgtat ctcatatggg agaggtacag
      841 ataaagggag cagagtcaga aaaatttata caaaacctag ttacaaatga tataagcgca
      901 ttaaagataa atgatattat atatacacct atgtgttatg aaaatggtgg agtagttgat
      961 gatttattaa tatataaatt tggtgaagaa gattatttat tagtaataaa tgcaggtaat
     1021 atagataaag atgtagcatg gataataaaa caaagtgaag gatacaatgt agatataaaa
     1081 aatatatcta gtgaagtgag tcaattagca attcaaggac ctaaagcaga agaaattctt
     1141 caaaaaatca cagatataga cttaaactca ataaaatttt ataagtcaat tccatcaaca
     1201 aaagtatgtg gttgtccttg tttagtttca agaacaggat atactgggga agatgggttt
     1261 gagatatact gtaaaaataa atatgtagaa ataatttgga atgaagttct aaaggttgga
     1321 ggagaagata tatgtccagc tggtctaggt tgtagggata ctttgagatt tgaagcagct
     1381 ctacctctat atggacatga aataaatgaa cacatatctc caattgaagg tggtttatct
     1441 atctttgtga aaacaaataa agaatcattt attggcaaat caattttatc aaaagaaaaa
     1501 gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc aaggtaaagg aatgcctaga
     1561 aatggatatg atataaggat tggtgataaa acagttggat ttgtaacaac aggatgtgca
     1621 tctccaacga caggaaaaat tttaggtatg ggaattatag attctgaata tgcaaaagta
     1681 ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc ctgctgtgat agttaagaaa
     1741 cctttttata aaaaataata taaaaaagat aatattatat taaacaaaga aaataaattt
     1801 tcatatatac ctgctacaag tgaagataaa tcaaaaatgt taaaagtagt tggattaaat
     1861 tcggttgatg aattattttc agatatacca gaagaagtaa aattaaaaag agatttaaat
     1921 ttggaaattg gaaaatctga attagaggta agcaaaatag taaaaagatt gtcagaagaa
     1981 aatttaagtt tagaagactt gacttgtttt cttggtgctg gtgcatatga ccattatata
     2041 ccatcaatta taaaacatat aacttcaaga tctgaatttt atacagcata tactccatat
     2101 caagctgaaa taagtcaggg aactttacaa gtagtttttg aatttcaatc tatgattgca
     2161 gaaattacag atatggaaat tgcaaatgca tcaatgtatg atggagcaac tgctgcaata
     2221 gaagcatgta taatggcaat gaatcagact agaaaaagta aaatagtagt gtcaaaaact
     2281 attcatcctg aaactttgtc agtattaaga acttacttac aatataaaga ttgtgagata
     2341 gtagaaattg acttttgtaa tgaatatggg actacagata tagaaaaatt aaaagcttct
     2401 gtagacaaag atacagcatg tgttcttata caaactccta atttctttgg aattattgaa
     2461 gaaatggaag aaatagaaaa gataactcat gaaaataagg ctatgttaat tatgagtgta
     2521 gacccaatat cattaggagt tttaaaaaca ccaggtgaga taggtgcaga tattgttgtt
     2581 ggagaagctc aatcacttgg aaaccctctt aattttggag gtccttatgt aggattttta
     2641 gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa tagttgggca aagtttagat
     2701 gtagaaggta aaattgcata tgtattaact ttacaaacta gagaacagca tgtaagaaga
     2761 gaaaaagcaa cttctaatat atgttcaaat caagctttaa atgctcttgt agcttcaata
     2821 tatatggcta ctatgggtaa agaaggattt aaagaggttg gtatgcagtc tatgaaaaaa
     2881 gcacattata cctacaataa acttgtacaa actggaaaat ataagccaat atttaaaggt
     2941 aagtttttca aagaatttgc tgtacaagga aatcttaata tagaaactat aaatgataaa
     3001 ctcttagaag aaaatatatt aggaggttat aatttagaat acaattatcc agaattaaaa
     3061 aattcaactt tgctttgtgt gactgagaaa agaagcaaag aggaaataga taagttagtt
     3121 ggaataatgg agggattata atgaaagaat acaatagttt gttgatagat atatctaaaa
     3181 aaggtaggaa agcatactca cttccaaagt tagatataga tgatataaaa atagaagata
     3241 tgattgaccc agatatggca agacaaagtg agttgaattt gccagaagtt ggtgaattag
     3301 aattagttag acattataca ttattatcaa ataaaaactt tggagttgat acaggttttt
     3361 atcctttggg ttcttgtact atgaaatata atcctaaaat aaatgaagat atggcagcac
     3421 ttccaaactt tactggaatg catccatacc aatcttcgga tacagctcaa ggttctcttt
     3481 ctttgatgta tgatttatca agaagacttg cagaaattac aggtatggat gaagttacac
     3541 tacaaccttc agcaggttct catggtgagt ttacagggct tatgattata aaagcatatc
     3601 atgaaaatag agatgaccat aagagaacca aagtgataat tccagattct gcacatggaa
     3661 caaatccagc aagtgcagct atggcaaact ttgatgtgat tcaaatagca tctgataaaa
     3721 atggagctgt agacataaat gtattaaaag aggttttgaa tgatgaagta gcagctctta
     3781 tgcttactaa tccaagtact cttggtttat ttgaaaaaaa tataaaagaa atagctactc
     3841 ttgtacatga agcaggagga cttttgtact atgatggtgc aaacatgaat gctattatgg
     3901 ggataacaag acctggagat atggggtttg atgtggttca tcttaatctt cataagactt
     3961 tttcaactcc tcatggaggt ggaggtccag gagctggtcc tgttggagtt aaaaaagaat
     4021 tagtaccttt tttaccaata ccagtaattg aaagaaaaga agataagtat gtactaaatt
     4081 atgatagaga aaaatctatt gggaaaatta aaaatttcta tggtaatttt ggagttcttg
     4141 taagagctta cacatatata ctaactatgg gaagggatgg acttaaagag gctagtgaaa
     4201 tggcagtttt aaatgctaat tatataaagg aaagtataaa agatgattat attttgccaa
     4261 tagatacctt gtgtaagcat gagtttgtat taggtggatt gccaagagga gaggccaata
     4321 taaaaactat cgatattgca aaaagactac tagattatgg ataccatcct ccaacaatgt
     4381 actttcctct tattataaat gaggcactta tgatagaacc tacagaaagt gagagtatag
     4441 agacattaga tagttttata gaagctatga aaagtgttgc taaagaggca aaggaagaac
     4501 cagaactatt gaaaactgct ccacataata ctttggtcaa aagagtagat gatgcaaggg
     4561 cagtaaagaa acctatacta acatggtcac aaagataaat agttaaaaag tagagattta
     4621 aagtataaag agaattaaat ttataagaaa aagctatcta acttaatcta gtttagatag
     4681 cttttttgta tattagtata tatggtattt cctatataaa gttttaaatt atattagtgc
     4741 ttatatagaa tattgatatg atgattatgt atagatatta ataagaattg ttgaatttat
     4801 cttgccttaa ctttatttac tcttttatat taagaaaaac
//
LOCUS       DAELJO010000246         4806 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELJO010000246 DAELJO010000000
VERSION     DAELJO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.248"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..536
                     /locus_tag="KPB88_003533"
     CDS             <1..536
                     /locus_tag="KPB88_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0055769.1"
                     /translation="GSEVLYYSNKGLEYATRICDKLGTVFKNRGAKLDKSLYILNSSK
                     PTAILIESFFCDNKEDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDG
                     EVDKIPATVVGWGYNDGKILICDIKDYVPGQTQNLYVIGGGACEKISSITKEHYTMIK
                     GNDRFDTLYKALDFIDR"
     gene            781..1410
                     /locus_tag="KPB88_003534"
     CDS             781..1410
                     /locus_tag="KPB88_003534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055770.1"
                     /translation="MFMKDDLNNDDIKKQICDILKVIRDTEDGIEILETIFDCHIKES
                     DEEELIKNIVFHAKESFNYDFSIILMYFSKQEFLSEKDINDSSLIEPLHILNKLKRKY
                     GYILNTIQRSSGVNSFLLKTITLSGSLDSQVCTLFLKRNDNEKFSLMLNSENLLTIHT
                     LSAQALDTMIDNKVYNFNIEALITYLKESQKVLNKISNIVDDFEKATNK"
     gene            1440..1862
                     /locus_tag="KPB88_003535"
     CDS             1440..1862
                     /locus_tag="KPB88_003535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055771.1"
                     /translation="MKELSSQEKWYNSNIVSAIIGAVIAIAVALLTIYITEKLKIKEE
                     KKNTIILITQTNQELFYNIYIGSKSNNNINHSKFRELVKHSSIIFIMPQNLKNEFSKM
                     FEIYSLIGDDFVARKDEVHTHCINIVKIIEEYGVDVFE"
     gene            1855..2214
                     /locus_tag="KPB88_003536"
     CDS             1855..2214
                     /locus_tag="KPB88_003536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055772.1"
                     /translation="MNNENNNENNIDEFINDSKQHNVDFENVKNTIKNQLADTDVLLD
                     RLCRMGKIRDMLVESAYIKKDSVMYETINKSISTATDLYSDNCHSIENIYTIHEKYEN
                     VISIAARVKACSGESAT"
     gene            2258..2554
                     /locus_tag="KPB88_003537"
     CDS             2258..2554
                     /locus_tag="KPB88_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055773.1"
                     /translation="MFKNENSFYPNNIFPSLDFTKIKAQLKNINYIYESDLIDCGSIC
                     IIENENYALSINSIGEINIYYNKEYKNKIQIIVDEIEQLFKSQVKNFRVSKFKN"
     gene            complement(2584..3087)
                     /locus_tag="KPB88_003538"
     CDS             complement(2584..3087)
                     /locus_tag="KPB88_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055774.1"
                     /translation="MNLTSWNQNRKLKKRFKGNKKNIKKYHEYYIKIYNHYNEIETVD
                     SIRKIEIEQTRIRGQLEKNNNPGNALYIALIAIVFSLLYNLSSNIVESYLSNLVFNIL
                     KIKCPNLDLLIQLICIIIFLAYVQSTSLSIAKPYRDDIQFYTTALDVLEDMKKKILRE
                     RKYKKRR"
     gene            complement(3425..3808)
                     /locus_tag="KPB88_003539"
     CDS             complement(3425..3808)
                     /locus_tag="KPB88_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055775.1"
                     /translation="MRKKLFLGIFIVLVIATIVVLNIKNDKNIFIGESEHWTAKFVTT
                     EEQSELEIKYKDSKDAVKQKFNYSYEFIDGEDAIIILKDAVLESGQEKIIKRQRPGLY
                     SNRESNYKVIISWNKNEETINLKKK"
     gene            3956..4285
                     /locus_tag="KPB88_003540"
                     /pseudo
     CDS             3956..4285
                     /locus_tag="KPB88_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4484..4699
                     /locus_tag="KPB88_003541"
     CDS             4484..4699
                     /locus_tag="KPB88_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055776.1"
                     /translation="MYINQTGFVSNGLGIKQQQRKDPTSNSFSYNAPSSWKPVGRDSL
                     QYNCGFESIATLVRGGSSWSFNFIVQP"
BASE COUNT         1923 a          524 c          696 g         1663 t
ORIGIN      
        1 aaggttcaga agttctatat tatagtaata aaggcttaga gtatgcaact agaatatgtg
       61 ataaactagg tacagtattt aaaaatagag gagctaaatt agataaaagt ttatatattt
      121 taaacagttc taaacccact gcaatactaa ttgaaagttt cttttgtgat aacaaggagg
      181 attatgagaa ggctaagaaa ctaggtcatg aaggtattgc taagttaatt gtagaaggtg
      241 tattaaataa aaatataaat aatgagggag ttaaacagat gtacaaacat acaattgttt
      301 atgatggaga agttgacaaa atccctgcaa ctgtagttgg ttggggttat aatgatggga
      361 aaatactgat atgtgatata aaagattatg tacctggcca gacacaaaat ctttatgtca
      421 tagggggtgg agcatgtgaa aagataagtt ctattactaa agaacattat acaatgataa
      481 aaggtaatga tagatttgat acactttaca aagcgttaga ttttattgat agatagatta
      541 tgaattaaaa aactttctac tcaaagtgta tatacacttt acctacactt tgcctacact
      601 ttatatacac tttgtagact atttttagat tcatatatta tatagtaaag ataaggatat
      661 cctaatataa attatataaa tataattcct tcaaaatata gataaatatt gaacaaaatt
      721 tataaaatct atggtactat tatagtgtag aataatagat ttatacaagg gggagtttgt
      781 atgtttatga aagatgattt aaataatgat gatataaaaa aacaaatatg tgatatatta
      841 aaagttataa gagatacaga agatggaatt gaaattttag aaactatttt cgattgtcat
      901 attaaagaaa gtgatgagga agaattaatt aaaaatatag tatttcatgc aaaagaaagt
      961 tttaactatg atttttcaat aattttaatg tacttctcta agcaagagtt tttatcagaa
     1021 aaagacataa atgatagtag tttaatagaa cccttgcata tactaaataa acttaagaga
     1081 aaatatggat atatattaaa tacaatacaa cgaagttcag gcgtaaactc ttttctatta
     1141 aaaacaataa ctttgtcagg gtcacttgat tctcaagtgt gtactttatt tttaaaaaga
     1201 aatgacaacg aaaaatttag tttgatgctc aacagtgaaa atttattgac aatacataca
     1261 ttatcagcac aagcattaga tacaatgata gacaataaag tttataattt caatatagaa
     1321 gcattaatta catatttaaa agagagtcaa aaagtattga ataaaatttc aaatatagta
     1381 gatgattttg aaaaggcaac aaataaataa tgagctctat tactacatat agctctattt
     1441 tgaaagagtt aagttctcaa gaaaaatggt ataattcaaa cattgttagt gcaattattg
     1501 gggctgtaat agctatagct gtagctttgt taacaatata tataactgag aagttgaaaa
     1561 taaaagaaga aaaaaagaat acaattatat taataactca aacaaaccaa gagctgtttt
     1621 ataatatata tatcggtagt aaaagcaata ataatattaa ccattctaaa tttagagaac
     1681 ttgttaaaca tagtagtata atatttataa tgcctcagaa tctaaaaaat gaattttcta
     1741 agatgtttga aatttattcc ttgatagggg acgattttgt agctagaaaa gatgaagtac
     1801 atacacattg tatcaatata gtaaagatta ttgaagagta tggagttgat gtttttgaat
     1861 aatgaaaata ataatgaaaa taatatagat gaattcataa atgattcaaa gcaacataat
     1921 gttgattttg aaaatgtgaa aaatacaata aaaaatcaat tagcagatac agatgtattg
     1981 ttagatagac tatgtaggat gggaaagatt cgcgatatgt tagtagaaag tgcatatata
     2041 aagaaagatt ctgtaatgta tgaaacgatt aataaatcta taagtacagc tacagactta
     2101 tattcagata attgtcattc tattgaaaat atatatacta ttcatgaaaa gtatgaaaat
     2161 gtgataagca ttgcagctag agtaaaagca tgctcaggtg aatctgctac atagaactat
     2221 atgatatata aaataaaaaa gtataggagt gaatggaatg tttaaaaatg aaaacagctt
     2281 ttatccaaat aatatatttc cgtcactaga ttttactaaa ataaaagctc aattaaagaa
     2341 cattaattat atttatgaaa gtgacttgat agattgtggt agtatatgca taattgaaaa
     2401 tgagaattat gctttaagca ttaatagtat aggtgagatt aatatttatt ataataaaga
     2461 gtataagaat aaaatacaaa ttatagttga tgagattgaa cagttattca agtctcaagt
     2521 taaaaatttt agggtatcaa aatttaagaa ttgatgatta agaaggtagc aactggttag
     2581 ttgttacctt ctttttttat actttctttc tctaagtatt ttctttttca tatcttctaa
     2641 tacatctaat gcagtagtat aaaattgtat atcatctcga taaggtttgg ctatacttaa
     2701 agaagtagac tgtacatagg ctaaaaagat aataatacat attaattgaa taagtaaatc
     2761 taaattagga catttaattt ttaagatatt aaatactaag ttagataaat atgattctac
     2821 aatattagat gaaagattat ataataacga gaaaactatt gcaattagag ctatatacaa
     2881 tgcattacca ggattattat ttttttctaa ttgtcctcga attctagtct gctcgatttc
     2941 tatctttcga atgctatcta cagtttcaat ttcattataa tgattatata tttttatata
     3001 atattcatgg tattttttta tatttttctt atttccttta aaacgttttt ttaattttct
     3061 attctgattc caacttgtca aattcataaa atcccccata aattcatata taatatactt
     3121 taatttataa cacacttaat aatatatatc agcaatttgt cgaacgattt taaataaaaa
     3181 tagagccagg atacaagaga aaaatttaca gagatttatt ctgatgatgt gtggtcaaca
     3241 atggataagg ctagataatt tgtaaagaca aaattataag ttttatattt ataaagataa
     3301 agctttgata tatttcatcc ataattttca aagacttata taaaaaggag tgttttattt
     3361 ttctaaacac tcttttttat ataaggattt caataatcaa tttttaaaat taataatatt
     3421 gttatcattt ctttttcaag tttatagttt cttcattttt attccaagat ataattactt
     3481 tataattaga ttccctattt gaataaagac ctggtctttg tctttttatt attttttctt
     3541 gacctgattc taaaactgca tcttttaaaa ttataattgc atcttcacca tctataaact
     3601 catatgaata attaaatttt tgcttaactg catctttact gtctttatat tttatttcta
     3661 attctgattg ttcttcagta gtaacaaatt tagctgtcca atgttctgat tctccaataa
     3721 agatattttt atcatttttt atattaagta caactatcgt agcgattact aaaactataa
     3781 aaatacctag aaaaagtttt tttctcatac tgagccctcc tttgaataat tatatgtatt
     3841 ataacatgaa aacagttatc taacatcatt attatgaatt ttctgaattt ttttgatatt
     3901 tatattctat catgatagaa tatgtggtat aattaataaa aaaggaggga ttagtatgaa
     3961 ttctaggttt aaaaaatctg caacaagtat tttatcttta cttgcaatga caatactatt
     4021 accagcaagt gtaaatgcta gtgaagtaga aaattcatct atgaaagtaa catattcaga
     4081 gaaacaagta acagatatga acaagttata tgatatggct aaaaatggaa tcactgatgt
     4141 taaaagtccc aacgaaaaag gtataatcaa aaatgaaaga acaggagaat ctatgtatgt
     4201 tgatgctata tcaacaacac aattattaga agtcaaacaa tctaaagatt taacatctaa
     4261 gacatattct aaaacagttt ttttagatac tgatacaata aagcccctta gaaaagcaag
     4321 tatatcagaa gacggatggg ataaaactgg tggtgtaaaa gcatctggta cagttacata
     4381 tacaatatca aaaggagatg gagcattcca atatatttct gtcagtaaag tttctggaaa
     4441 ctggtctatt gcagatggtc agattcgtgt aacaaataga aaggtgtaca taaaccaaac
     4501 tggatttgtt tctaatggat taggaattaa gcaacaacaa aggaaagacc caacatcaaa
     4561 ttctttttca tataatgcac cttctagttg gaaaccagtg ggtagagatt ctcttcaata
     4621 caattgtggg tttgaatcaa ttgcaacatt agtaagagga ggaagctctt ggtcattcaa
     4681 tttcatcgtt caaccttaaa acattttaag tgaattttat taaaattaaa gatatattga
     4741 caccataaag atataataaa aaataaaact atatagtata agttgtaata ataaaacgaa
     4801 cgaaaa
//
LOCUS       DAELJO010000247         4776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELJO010000247 DAELJO010000000
VERSION     DAELJO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.249"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            314..1789
                     /locus_tag="KPB88_003542"
     CDS             314..1789
                     /locus_tag="KPB88_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0055777.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1876..2289
                     /gene="speD"
                     /locus_tag="KPB88_003543"
     CDS             1876..2289
                     /gene="speD"
                     /locus_tag="KPB88_003543"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG0055778.1"
                     /translation="MKFETLGRHILVEYYNCDENILKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2319..3170
                     /gene="speE"
                     /locus_tag="KPB88_003544"
     CDS             2319..3170
                     /gene="speE"
                     /locus_tag="KPB88_003544"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG0055779.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3160..4038
                     /gene="speB"
                     /locus_tag="KPB88_003545"
     CDS             3160..4038
                     /gene="speB"
                     /locus_tag="KPB88_003545"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG0055780.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNILNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4218..4418)
                     /locus_tag="KPB88_003546"
     CDS             complement(4218..4418)
                     /locus_tag="KPB88_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0055781.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1952 a          516 c          787 g         1521 t
ORIGIN      
        1 attaaaaata gtaaactcaa aagcttacta aaactcaaaa aagtaaaaaa accaaaataa
       61 taaaaaataa gaaagtttat aaatactaag ttttaaaaaa attaatataa acaacaaaaa
      121 attaaaaagt ttataaatat tgatatagct aatgtatatg tttttgagta acttcaaaaa
      181 aacacttgta aaaatgctga gatgttaata taataaatat catgagttta ataaaagtaa
      241 aaataaaaaa tagaaataat aaataataaa tattaaaaat atttgaatat agagaggaga
      301 tgaatttgaa atcatggata gcgagtttgc aaatgcagtt gaattaagct atgagtgtgc
      361 tccattatta gagtcgttaa aggaatactc agagaaagat atagcttgtt ttgatgtgcc
      421 tggacatgta aaaaatagag gtgtagctat attaaataaa tatctaggtg aaagtctcat
      481 gaaaatggat ataaattcat caccaactat ggataatgta tctgcgccaa atgggattat
      541 aaaaaatgca caagatttgc tagcacaagc atatatggct gatgaggcat tctttataac
      601 gaatggcaca actcaagcaa tacatgcaat gattttgagt gttataaaac caggtgaaaa
      661 agtattactt ccaagaaata tacataaatc agtaataaat gcgttaatac tttgtggagg
      721 aataccaata tttatacaac cagaatttga tgaaaaacta ggtataagtt taaatattac
      781 tcttgaaaaa gttagaacag agatagaaaa agattgtaat ataaaagcat tatttttcct
      841 taatccaact tactatggag tttgtgcaga ccttgagagt attatagaac tatgccataa
      901 aaataatgtt ttagtgttag ttgatgaagc tcatggtgca cattttccat ttcatttaga
      961 tttacctcct tctgcaataa gtttgggagc tgatatggta gctgtaagta tacataaaac
     1021 tggaggagca ttgacacaat cttcagcact cttattgaat agagataatg taagttttga
     1081 aaaggttctt caatcaatca atatgctaca atcaacatca gcatcttatt tgcttatggc
     1141 aagtatagat ggtgctagag ttaatctagt tgagaatggt gaaaagcaac tgtcaaaagc
     1201 attaaatcta tctaggtatg caaaatctaa attaaacaaa attgatggaa taaaagtttt
     1261 atctacagaa atattgaaac aaaaaggtgt taaatttata gatgaaacaa aattgtgtat
     1321 aaatgtaaaa gaattaaatt tgactggatt tgaagtatac gatttattat ataaaaattt
     1381 ttcaattcaa gtagaattgg gagattcata taatatactg gcacttgtgt ccattggaac
     1441 aaataaatca gatatagata gattagtaaa ggcactttct ataattgcaa agatttatag
     1501 aaaagaatca acattaaatg aatttaatat ggttcaaata aatccaatta ttaaacttaa
     1561 tccaagagaa gctttttatg caccaaagga aagtgtagaa atcaattcat gtatagatag
     1621 aatttgtgga gaatcaataa tggcatatcc accaggtata ccgattattg ctccaggaga
     1681 attgataaca gaagaaatta tggagtatat tattttctta aaaaatagca atgcgtatct
     1741 tacagatgta caagataaaa acttagatag aatactagta ataaaataaa ttactttatt
     1801 ttgcaagggc aagtgtttgt taagtagagt ttatcaaata gaacttatta aaagtataaa
     1861 ggagttgggg ttgaaatgaa atttgaaaca ctaggaagac atatactagt agaatactat
     1921 aattgtgatg aaaacatact taaagaccca cgtcttatag aaaattttat gaatgattca
     1981 gcattaaatg caaaagcgac aattgtggat tctgtattcc accattttaa tccttggggg
     2041 gtttcaggag ctgttataat tgctgaatct catttgacaa tacatacatg gccggaatat
     2101 ggttatgcag cagcagattt ttttacttgt ggtgatatag acccatggaa aagttttgaa
     2161 ttattagagc aacttttaaa gtctgaaaga agcgagtcta cagaaattcc aagaggctta
     2221 actacaaaaa ttaaaaaata ttcaaaaaaa gatttaggta agattactca taaaccagaa
     2281 gcagtataaa acatacaaga gaaagtgagg attattatat ggaactttgg tatacagaag
     2341 aatggacaga aaatgtaaga ttttctataa aagtaaataa acatctattt gaaggaaagt
     2401 ctcaatttca aaggatagat gtgtttgata gtgatgaatt tggaaaattt ctaacaatag
     2461 atggattaat gatggtaaca tataaagatg aatttatata ccatgagatg ataactcatg
     2521 ttcctatggc tacaaattta aacataaaga aagtattagt cataggtgga ggtgatggag
     2581 gtacagtaag agaattaagc cgttatcctc aaatagaaaa gattgacatg gttgaaatag
     2641 ataaaatggt tgtagatgta tctaaggagt atatggatat atgctcatgt aaattagatg
     2701 ataaaagagt gagtttatat tttgaagatg gtgttaattt tgttaagtgt gcacatgata
     2761 aatcatatga cctgataata gtagattcaa ctgacccaat aggaccaggc gaagggcttt
     2821 tctcaactga tttttataag gattgttata gaatactgac tgatgatgga attttagtaa
     2881 atcaaagtga aagtccgtat tttgacttta atgctaaaga gatgaaaaga gctaataaaa
     2941 aattaaaaca gatatttcca atatcagaag tttatcaagc acatatacca acatatccat
     3001 ctggtcactg gttgtttgga tttgcttcaa aaaaacttaa tccagtaaaa aatcaagata
     3061 gaaatggatg ggagaaatta agtttaaaaa ctaaatatta taatagtgat atacatttag
     3121 gttcgtttat gttacctcaa tacgtgaagg agatgctaga tgaagaataa tttctatcat
     3181 atgaacactt ttatgagtat ggacaaaaat tatgaagaat ctaatcttat agtatttggt
     3241 gttggatttg atggaactac ttctaacaga cctggggcta gatttgcaag tagctctatg
     3301 agaaaagaat tttacggtct tgagacatat agtccttttt tagatttgga tttagaggat
     3361 tataatatat gtgattatgg agatttagaa attagtgttg gaagtacaga acaagtctta
     3421 aaagaaatct atcaagagac atataagatt gttagagatt caaaggtacc ctttatgatt
     3481 ggaggagagc atttagttac attaccagcc tttaaagcag tacatgaaaa gtataatgat
     3541 atatatgtaa ttcattttga tgcccatact gatttgaggg aagaatataa taatagtaaa
     3601 aattctcatg caacagtaat taaaagaata tgggatattg taggtgataa taaaatattt
     3661 caatttggta taaggtctgg gacaaaagaa gaatttaaat ttgctacaga agaaaaacac
     3721 acatacatgg aaataggagg aatagatacg tttgaaaata tagttaatat actaaatgga
     3781 aagaatattt atctaactat agatttagat gtattggatg catctgtgtt tccaggaaca
     3841 ggtacaccag aacctggtgg tgtaaattat agagagtttc aagagatttt taagattata
     3901 aaaaactcta atataaatat agttggttgt gacattgtag agttaagccc tgattacgat
     3961 acaacaggtg tatcgacagt tatagcttgt aaaatcttaa gagagttatg cttaataata
     4021 tctgataaaa ttaaatagag tattttatat aaatagtata tattataata taagaagtta
     4081 ttttaaaaaa gttagtttaa gtttgacgct tttctaaagt aacttcttat ttttgtaatt
     4141 aaaaataaat ataataaaca acaaaaaaga gccctcgata gctccttttc taaaatatat
     4201 atatttaaat taaataatta tattctattt acgttttcag cttgaggtcc tctattacct
     4261 tgagttatat caaagctaac cttttcgcct tcttctaaag ttttgtatcc atcattttgt
     4321 atagctgaga aatgtacgaa aacatcatct tctccctcta cagatatgaa cccaaatcct
     4381 ttttcattgt taaaccattt tactattccg tttttcatta taagaaaacc tccgcattat
     4441 taaaaaatac aaactaaaaa aataagtctg ttctattaat ataacataaa ttttaaataa
     4501 aagcaaataa aattttttaa aaaattaatt acttttagaa tttaataaaa attgttaaat
     4561 tatacagtga gaagtattgt aaaatttgac gaaaatgtaa atgtagataa taaatttaat
     4621 atcaaaaaat gtaacaaaaa ggtatgtaag taaaaagtaa aattaaaata tataataaaa
     4681 aatattatat attagattga aaagtaaaaa taaaaatagt gaaatttcag aaatatcaat
     4741 aatattatat aataaaacaa aaaaacacta tatata
//
LOCUS       DAELJO010000248         4771 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELJO010000248 DAELJO010000000
VERSION     DAELJO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.250"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            187..2688
                     /locus_tag="KPB88_003547"
     CDS             187..2688
                     /locus_tag="KPB88_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG0055782.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2848..3516
                     /locus_tag="KPB88_003548"
     CDS             2848..3516
                     /locus_tag="KPB88_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055783.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3500..>4771
                     /locus_tag="KPB88_003549"
     CDS             3500..>4771
                     /locus_tag="KPB88_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0055784.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFK"
BASE COUNT         1986 a          497 c          870 g         1418 t
ORIGIN      
        1 cttttctatt aaatacaaat aaatataact aatacattta tttattaaaa aaatattgat
       61 ttaatatacc tatagggggt ataataaaaa tatagatttc taaataagta atttatcaat
      121 attacatatc aaaataaaac gttacaaatt agctgaaaat atattgaaag aaaggaagat
      181 tttattatga gttcaaataa agtggtagaa agttataaaa ttacaggtat gacatgtgca
      241 gcttgtgcaa aggcagtaga gagagtaact aagaagatgg atggagtata tgaccaaagt
      301 gtaaatatag ctactgaaaa acttaaaata gaatatgata attctaaggt taattttgat
      361 gatataaagc aggttgtaga aaaagcagga tatggaatta taaaggaaga aagtaataaa
      421 aaaattgata tgaaaattga tggaatgaca tgtgcagctt gtgcaaaagc tgtagagaga
      481 gtggtaaaaa aattggatgg agtagaaagt attagtgtga atattgcaac agataaagct
      541 aatatagact atgacccatc aaaagtaaaa ttatctcaaa taaaagcagc tattgaaaaa
      601 gctggatata aaccaataga agaggtaaaa aataaggttg atgtggatga agataaattg
      661 agaaaagaaa gagaaatgaa gtctttattt gttaaattta tagttgcaat agtatttgca
      721 gtacctctgt tttatatagc aatgggacct atgataataa agcctatagg gccatggcct
      781 ttgccagaaa ttataaatcc tatgacaaat acttttaatt atgcattaat tcagttaatt
      841 ttagtaatac cagtaatgat agctggttat aaattttata taaatgggtt taaatcactt
      901 ttttctttaa gccctaatat ggattcttta gttgcaatag gaacattagc cgcattttta
      961 tatagtctat atacgacact acaaatagcc aatggacaaa ttcaaggtat gcaccatcat
     1021 cagttgtatt atgaaagtgc aggaataata atagcactta tcttacttgg taaatattta
     1081 gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa agcttatggg gctacaacct
     1141 aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa ctcctataga agaagtagag
     1201 ataggagata tactattagt aaaaccagga actaaaatac cagtggatgg agttgtaata
     1261 gaaggatata catcagttga tgaatcaatg cttacaggtg aaagtatacc agtagaaaag
     1321 aatgttggaa gtaaggtgac aggagctagt attaataaaa atggtgttat taagtttaag
     1381 gctgaaaaaa ttggtggaga tactgctctt gcccaaataa taaagttagt tgaagatgca
     1441 caaggtacaa aagcacctat tgctaaactt gccgatactg tatctggata ttttgtacca
     1501 atagtaattg caatagctgt agttgcatca ttattgtggt ttttaatagg aggaaaagat
     1561 atagtatttg tacttactat atttatatca gtacttgtaa ttgcttgtcc ttgtgcatta
     1621 ggattagcaa caccaactgc tataatggtt gggactggaa agggcgcaga aaatggaatc
     1681 ttgataaaag gtggagaggc actagaatct gcacataaag taaatacagt gatatttgat
     1741 aaaacaggta ctataacaga aggaaagcca aaagtaacag atatagtatt aaataataat
     1801 gtaaaagaag aatatcttat caagattgct tcaagtgcag aaaaaggttc agagcatcct
     1861 cttggagaag caatagttaa gtatggagaa gagaagaata taaaatttga aaaagttgat
     1921 aattttaaag ctataccagg tgctggtata caagttacta ttaatgatga aagtatatta
     1981 cttggaaata gaaagcttat gaatgacaat aatataaagc ttggggattt agaagaaaaa
     2041 tcaaatatat tggcaagcca aggtaaaaca cctatgtata tagctgtaga tggaaattta
     2101 tcaggtataa tagcagttgc agatgtagtc aaagaaagta gtaaaaaagc aatagaaata
     2161 cttcatgata tgggaataaa agttgcaatg gtaactggag ataatgcgaa aactgcaaat
     2221 gctatagcaa atcaagtagg tatagatatg gttttggcag aggttttacc agaagataag
     2281 tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg ttgcaatggt aggggatgga
     2341 ataaatgatg ccccagctct tgcaaaagca gatataggta tagcaatagg aagtggaacg
     2401 gatgtagcca ttgaatctgc ggatatagta cttatgaaga gtgatttaat tgatgttcca
     2461 acagcaatta aacttagtca tgaaactata aaaaatataa aacaaaacct attttgggca
     2521 tttggatata atacaattgg tatacctgta gcagcaggaa tattatatgt atttggagga
     2581 cctttattaa acccaatgat tgcagcagca gcaatgagct taagttcagt ttcagtcgta
     2641 tcaaatgcac ttagacttaa aaacttcaag gcatataaga gagattagat taataaatta
     2701 aataaaatgt atccaaaaga gctatcttta aattaaaagg attaatttac aaattaagat
     2761 agctcttttt tcatgtaaaa ttcacaacaa taagataaaa tataagtaat atacgaaatt
     2821 ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa tacttgtaat tgaagatgat
     2881 tcaaatatac aagagttaat atcagaattt ttaagtgcag aaggttatca agttgataca
     2941 gcaaatgatg ggttggaagg tatacaaaag tttaagcaag gaagttatga tttagtaata
     3001 ttagatataa tgatgccaaa cttagatggt tatggagttt gtaaaatgat aagaaagtca
     3061 tcaagcgttc caattatatt tttaactgct ttaaatgatg aaggagacca acttaaaggg
     3121 tttgacttag aatgtgatga ttatataaca aaaccatttt catttaatct tcttataaaa
     3181 agagtagaag caattttaag gcgaagtaac aagactataa atgacaaatt tatagttttt
     3241 gaaaaattaa aattagattt aaacacatat attgcagaga tagatggaga acctatagaa
     3301 cttacactaa aagagtttaa tatattaaaa gctttgatag aaaagtatcc acaagttata
     3361 actagggaag gtcttttgga tagtatatgg ggttatgact attatggaga tactaggatt
     3421 gttgatgcac atataaaaaa tataagaaaa aaaatatcat taccatatat taaaactgta
     3481 aaaggaatag gatatacact tgagaaggat atttgataag tgggaaaagt taagtataaa
     3541 gtataagtta ttttcaataa cgacatcatt attaatagca ttagctttaa taatatatct
     3601 aattttatat tttttattgc catcatatta tcatgagtat aaaattgagt ctcttcaaga
     3661 aagtttgaaa tctttagtag atagttcaat tcatttcgat acatatactt tagaagaaag
     3721 actttattat atggctaaag accaaaattt ggctatttta ttaaaagata accaaggtaa
     3781 aattgtatat ggtaagaatg aagtagttat tttaagatat agtaagtaca tgattaatag
     3841 tcttgaggat gagtatagaa cttctatacc tatatatact aaagatgcaa aagatggtcc
     3901 atatacttta gaactcgtta tgccattaca accaattgac gaagcaaacg aagttataag
     3961 aaaactaatg ccatacatca tatcaatagc aatattgata gcgattattg gagcatatat
     4021 atattcaatt gtaataacaa agcctcttat aaacataata gagagtgaga gagaacagga
     4081 atatagaagg aaggattttg ttgctacaat atcacatgaa ctaaaaactc caataaccat
     4141 aataagtggt caaattgaag gtatgattta tagtgtaggt aagtataagg atagggatac
     4201 ttatctgaaa aaatcgtatg agtgtacaca agaattaaaa gatttagtaa atgaaatgat
     4261 agaggtttca aaatcagaaa tacttgaaaa agatttgaaa ttggtatcaa taaatataag
     4321 tgaattatta aatagacttg taaagagaca agtattttta atagaagaaa aacatatgaa
     4381 aactatttta aaaatagaag aaaatttgga aattaaggca gaccaagaga gaataactaa
     4441 ggcaatcaat aatataataa ataatgccat aaaatattct cctgaagaat cagagcttat
     4501 aattagactc tatgataaaa ataaacgaat aagtaaaaag acttctaatc aaagagtggt
     4561 tttagaaatt gaaaatactg gtgtaactat cgagaaacgt tatttagaag aaatatttaa
     4621 tccattttat agaatagaaa aatctcgttc aagaaaaact ggaggaagtg gacttggact
     4681 ttatatagtc agccaaattt ttaaaagtca tgggtttgat tatagcataa aaaacaaaga
     4741 aaactctgta gtattcacag tagaatttaa a
//
LOCUS       DAELJO010000249         4738 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELJO010000249 DAELJO010000000
VERSION     DAELJO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.251"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            190..1341
                     /locus_tag="KPB88_003550"
     CDS             190..1341
                     /locus_tag="KPB88_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG0055785.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKKVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1387..2562
                     /gene="thiI"
                     /locus_tag="KPB88_003551"
     CDS             1387..2562
                     /gene="thiI"
                     /locus_tag="KPB88_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG0055786.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2698..3501
                     /locus_tag="KPB88_003552"
     CDS             2698..3501
                     /locus_tag="KPB88_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0055787.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3744..4385
                     /locus_tag="KPB88_003553"
     CDS             3744..4385
                     /locus_tag="KPB88_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0055788.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1905 a          449 c          918 g         1466 t
ORIGIN      
        1 tagggtatag tataagtata ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac
       61 tagcttttca tgaaataata ttttataatt taagattgtc tttttaataa agattaacaa
      121 aaaaaatact ataattagtg gtataatagt tagagttgaa gattttaaat aaaggtaggt
      181 gtaattaagt tggaaatata cttagacaat agcgcaacta caaaacctta tcaagaagtt
      241 atagataaaa tggtttatgc acttaatacg gaatatggaa atccttcttc tgtccataga
      301 aaaggtgtag aagttgaaaa agctataaaa aaagtaagac aagatatagc taaatctcta
      361 ggagcgaaag aaaaggagat ttattttaca tcaggtggta cagagtgtaa taatactata
      421 ataagaggta ttactagttt aaataaaaaa agaaaaaatc acataatatc tactaacata
      481 gaacatcctt ctgttttaaa tacattaaaa gatttagaag aagatggttt tgaagtaact
      541 tatttagagg tcggaaaaga tgggaaaata aatatagagg atttaaaaaa tgcgataaag
      601 tcaactacat gcttagtaag catgatgcat gttaacaatg aaataggaac tatacagcca
      661 ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa aaatttattt ccatgttgat
      721 gctattcagt catatggaaa aattaacttt agaccatcta aatacaatat agattttatg
      781 agtgtaagtg cacataaatt tcatggtcca aagggaattg gatttatgta tataaaagaa
      841 aataatcgcc ttaagcctat gttaacaggt ggtggtcagg aaattggaat taggtcggga
      901 acagaaaatg tacctggaat atatggtctt ggagaagctg ttagaatact aaacaaagat
      961 ttagatgctg ttatttcgaa agtagataac ttaaaaaata tattaaaaaa tgaaataatt
     1021 gataatatag aagatataaa aatcaattct ccagaagatg gagtatgtca tatattaaat
     1081 gtatcattta gagggacaaa aggagaagtt ttacttcatt acttagagca aaaaggtatt
     1141 tatgtatcaa ctggctctgc atgttcttct aaaaagaaag gtagttacgt attaaatgct
     1201 ataggtctta caaatgaaga aataaatgga acaataagat ttagtttatc tgatatgaat
     1261 acagaagaag aaatgttaga agctgtgaaa gttttgaaag aatcaatttg tgacttaagg
     1321 tcaataatga aaagaaaata aagaatactg tataaatagt gataataaat tagtttaaga
     1381 ggtgaaatgg tgtataatat attaatagta aagtatggag aaattggagt aaaaggaaag
     1441 aatagatata tatttgagaa taggcttata agaaatataa gaaatatgtt aaaaccaatt
     1501 ggaagattta atgtatataa agagtatgga agaatatatg ttgatttaga agactatgat
     1561 tatgaagaag taattgaaga agttagaaaa gtatttggga tagtgggtgt gtgccctgga
     1621 gtaagagcta aaaaagatta tgatacatta aaagaaatag ctttaaaaat gttagaagaa
     1681 aaaatagagg ctggatataa aacttttaag gtagagtcaa gaagaggaga taagtctttc
     1741 gggttgactt ctcaagaaat gagtatggat ataggtggat acttattatc aaaggtagga
     1801 gataggatta atgttgatgt aagaaatcca gaagttaaaa taaagtgcga atacagagaa
     1861 tttcatacta tggtctatag tgatactata cctggatatg gtggattgcc acttggaacg
     1921 aatggtagag ctatgtctct tttatcagga ggtatagata gcccagtagc tacatggatg
     1981 gttgcaaaaa gaggtatgga ggtagaggca gttcactttc atagttatcc atttacaagt
     2041 gaaaggtcac aggaaaaagt taaagattta gctaaaatac tggcaaaata ctgtggtaga
     2101 gtaagacttc ataaagttaa catattagaa atacaaaaag caattggtga gaattgtaat
     2161 gaagaagaag ctactattct ttcaagaaga tttatgatga ggatagcaca gagactttct
     2221 gaaaaaagac attgtgatgc tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa
     2281 actatacaag ggcttacttg tactaatgcc gttgtagact taccagtgtt tagaccactt
     2341 atagcaatgg ataaatcaga tatagttgat atagccaaaa aaatagggac ttttgaaaca
     2401 tctatagtgc ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc cgtcactaaa
     2461 cctagattag agaaaataga aaaatctgaa acagcacttg acattgaaaa attagtacag
     2521 gatgctattg acaaaataga ggtagaggat attgagtttt agcatacgag ttatttaatt
     2581 attaaattga atattgagta tattagtagt tctgttgaaa ggatttatgt agacaattta
     2641 ttaatatgta gtatttaaaa ataagtacta taattttatt taatgggaat gattattatg
     2701 aataaaaagg gttttacatt aattgaattg ttggtagtta tatctataat aggcatttta
     2761 gttatagtag ctgttccagc gttatttaga aatatagaaa aaagtaaagc agttacatgt
     2821 ctttctaata gagaaaatat aaagactcaa attgttattg caatggctga ggaatcaagt
     2881 aaagacaaga atgaagtcat aaaagaggta ttagaaaaca aagatggtaa gtactttgaa
     2941 acagaaccaa agtgtaagtc aggtggaata tattcagcaa cgtttgatga tggttatgat
     3001 ggaataactg gaatagaaag cattgcaaaa gtgtatgtta cttgtacaaa acatccagac
     3061 ggtgttgaaa tggctaggga tgtacatcaa agtatgaaag atttgattgc atcatttgca
     3121 caagaccctt ctataatacc aggagcttca aagggcaatg atgattttag aaaatattta
     3181 ttagacaata aatataaaaa tgggtggcct acaattccag atgaatttaa ggcaaaatat
     3241 ggattaagta aggatacact atatatacaa ccatatgcat ataatcctac taaatctgat
     3301 gctactgtag ttgtatttgc aaataataag actgggggta attggtatac ttccctagtt
     3361 tacgattatg atgaaggtag atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg
     3421 tcatgggatg ttgacacaga tagtgttaag tctgtaaaaa cagagattca ttctaaagag
     3481 ggatggggtc ctttaaatta atatatcatg ttttattaat ctggtattga ttaaactata
     3541 gaaaagagca gtttctatag atgacgaata ccagattttc atttatatat ataattgaga
     3601 tatattaaaa gtgtttatat ttttaagtct tcacatacaa gctaaaaata attataataa
     3661 tattgtaaag aaatttaatt ataagattaa ttttctgaag atttagtatt tgtactaaga
     3721 aatacataaa aaggggaata gatatgaata ataattttca agacaaccaa acaataaatt
     3781 taatacaaag tcgtagaagt atacgaaaat ttacgacaga acaaattagt gatgagcaag
     3841 taaacacatt attacactgt gcatttgcag caccttcagg atgtaataaa cagccttggc
     3901 acataactgt tgtgcaagac cagaaacttt taaaagagat tagtgatgat actttatcta
     3961 ggattcatga ggtgagtaat gttgagataa ataaaaactt taagttattt tatggtgctc
     4021 caaccgtttt atttatttct tatgatgaaa gtagttcatg ggctccatat gatataggaa
     4081 tacttacagg gaatataaca acagctgcac aagcactagg gcttggaagt tgtataatag
     4141 gaatggttag aggtttattt acacctgttg aacaagggga tattgaaggt cttgtgagtg
     4201 tattggataa agaagatgta aaagaatcag agtcaataaa gatgaagttt gatacaaata
     4261 aaaaatacag agaactactt gatataccag aagggtattc tgtaccattt ggtattgctg
     4321 taggtattcc agatggaaat ttaccaaatg ctagagaggt agtttacaaa gtttccagag
     4381 tttaggataa agtattgcaa gaaccaatca gttaattgaa gaattaaatc agtaatatac
     4441 aaataaattg atggaagagg aagctattta aaaagtagtt tagatttttc gttgtaaaat
     4501 aattaaataa agaataaggt gataagtatt ttatttatta tcttattctt ttttattttt
     4561 gttttgtaga atgataaaaa taaggattaa gtgtaataaa atagaaaaca agtatgttaa
     4621 ttttaagtat atgctttaaa atttatccaa gttaaaaatt taagatatct tttcagtata
     4681 atggaaaaaa tgtcaaaata ttgaaatagg gaaaaaatag tgatataata agaaaaaa
//
LOCUS       DAELJO010000250         4709 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELJO010000250 DAELJO010000000
VERSION     DAELJO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.252"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            398..4540
                     /locus_tag="KPB88_003554"
     CDS             398..4540
                     /locus_tag="KPB88_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG0055789.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATVSSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIVNTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTDEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         1796 a          698 c          810 g         1405 t
ORIGIN      
        1 tttacgatat tttgtttgat gatttataat ataaaagcat actcttaatc ttcaaaattt
       61 aaaaagagta aaaaataaca aaaaaatcac tgacatttca ctgacaaatt gcaattttgt
      121 tattataaat acattgaaaa tacttgatta ttgaaaaatg gcataaaata atacatttta
      181 tgccatttac tattatttga tagtttttaa cttatgacat aagatatttt ttagcatata
      241 caataaaata taaaaaaatt gatattgcaa ataaaaaaaa tacatattag atgttataga
      301 aaaaaagcgc acttataggc ttaaaatgtc acataaaaga gttttaaaga ataatatatt
      361 atgaacacaa attttgtaat cacaaaggag tgataatatg gcaacagtat caggacaaat
      421 agtttttgat agaaatagaa gtgctaccat tgattctgga gactcaggaa tagccaatat
      481 accagttgta ttacaagata tagcaactgg agttagactt gtggttctca cagatgcatc
      541 aggaaattat gtttttacta atgttccaaa tggtaactat agaattgtag aagcttttgg
      601 aactacagga ggagtaccaa caccaggaaa ttttgcaaca gcgatagtag gtccagttcc
      661 aattgcagaa atgccaccta ttagttctgt gacaaatcct cctattggtt caacaaactt
      721 agatggtaca acacctaaca cactattaat aacagtagca ggttcagata taacaaatca
      781 aaatatatta aatgggcctg tgatttatac acctatagag aatatattgg attcttgcgt
      841 atctgtatct aatataaacc ttattacaga tgcagataac ggaacattcg gtttttttcc
      901 accaggtaca ccaagtaata caggacctga aatagaacct tacccaaatg tgacgccaga
      961 ttttacgtat gttgtaccag acccaactaa gtttacacct ttagatggag aatatacagt
     1021 acaaaatatt atgacaaatg caatgagtaa tgttatagga gcttggtggc gtattgctga
     1081 ccatactaca ggaaatgaaa caggaagaat gatggtggta aatggtttta atcctggagc
     1141 tgtatttttt aaatcaacag tacaagttac acctaacact aattatttat ttagcacttg
     1201 gattttaaac ctttttaagg taacaggata tccaccacca cagctaggtg taagaatatt
     1261 tgaccaaaat aataatatac tatatcaagc aacattagga gctcaaattc cagttaatac
     1321 aaacgcacca gaatggaaag aagtaggtac tgtgattaat tctcaaaata ataatcaact
     1381 tacagtagaa ttttttagtg aaggagaaag cgccattgga aatgactatg ctatagatga
     1441 tgtttcttta agagaaatag ctgttccaac ttttactcca ataaaaacaa gtaacacaga
     1501 aactgctaat gtaggtgata taatcacata tacagttaca ttagataata cttgtgatag
     1561 ccctttaact aatgtatttt ttagggattt aattccaaat ggtttagagt ttatacctgg
     1621 aactgtaacg gttgatggag tatctgtacc aggagtagac cctaatacag gtttcccact
     1681 tccagatatt ggaggaggaa caggggttga agtaactttt gatgttgtgg ttgaaagtat
     1741 tcctaaccca aatcctacta ataatatagc taatatagat tactcttata cacctgtaga
     1801 aggcggtata ccaaatgatt tttcagtaga ttctaatcca gttcctgttg aagtaatctc
     1861 agcagatatt gaagttacaa aattatctga acctactata gtgaatccag gagaggaatt
     1921 aatatacact ataaaagtag taaataatgg accattccca tctgaaaatg tggttttaac
     1981 agatgatgta ccagcttcta ttataaatcc agagtattca ttagatggag gagtaacttt
     2041 tcaaccatgg actggaagtt taaatatagg gacattagaa gtaggagaga caagagttat
     2101 tataattagg ggtattgtaa atccatcaac agtaggaatt ataacaaata cagctgtagt
     2161 atcttcaact acagcagacc caaaccttaa taataataca tctacaatag agacagaagt
     2221 gaatttatta gcagatattt tagtaatgaa aacagcagaa cctaattcag ctgttccagg
     2281 tactttattg agatatacta ttcaagtaga aaacttagga ccagcaaatg ctgaaaatgt
     2341 aatattaaat gatgatattc cagcttctat tataaatcca gaatattcat tggatggagg
     2401 agcaagtttt caaccatgga atggaagtct taatatagga actttaaact ctggaatttc
     2461 attaacagtt ttaattcaag gaacagttag tttaaattct tcagaatata tagtaaatac
     2521 agcaacagtt tcttcaacta cacctgaccc agatttaagc aacaatatat ctaccattat
     2581 tacaccagtt aacccacaag caggtatttc tattataaaa gtagctgacg aggatgtagc
     2641 tgttccagga gaagaatttg tatatacaat tgaaattttt aatgaaggac catctaatgc
     2701 tacgaatgta gttttaacag atgatatacc agatgttatt ttaaatccag aatattcatt
     2761 ggatggagga gcaactttcc aaccatggac tggaagtctt aatataggta cagttgcacc
     2821 agggcaatta ataagaatta tcataaaggg attagtaagt tcaactgcta caggagatat
     2881 tactaataca gcagaagtaa gtgcagatgt accagaacca gttacggatt catcaacagt
     2941 gacaactcct atagttccat cagcagatat agaagtaata aaaactagca atatggatac
     3001 agcagttcca ggagaaactt ttagttatac tataacagtt acaaatttag gaccatctgc
     3061 tgcacaaagt atcgttttga cagatgatat accagatgtt attttaaatc cagagtattc
     3121 attggatgga ggagtaactt tccaaccatg gaatggaaat cttagtatag gcactttgga
     3181 tgctggagaa ataagaagta ttataataag aggaacagtg agccagacag cagtaggtac
     3241 aattataaat acagcaacag taagttcacc tacacctgac ccaaatccag ataataatac
     3301 ttcaacagat gaaacagata taagtccatt agcagatatt tctgtgataa aaggcaacga
     3361 accagttgct ataccagggg gaagatttat ttatggaatt gaaattgcaa atgcaggacc
     3421 atcttttgca gagaacgtaa ctttaacaga taatatacca gcttctattt taaatccaga
     3481 gtactcaata gataatggag tgactttcca accatggaat ggaagcctta atataggtac
     3541 tttagatgct ggagagataa gaaatatcat aataagggga acagtaagtc aaacagcaat
     3601 aggcacaatt gtaaatacag caacagtaag ttcaactaca cctgacccaa atctaaataa
     3661 caatacttca acatcagaag cagaggtaag ttcatcagca gatatttctg tagtaaaacg
     3721 aagcaatcag actgttgttg taccaggaga tgtactagac tatacaattg aggtgagaaa
     3781 tgcaggacct tctactgctc agaacgtaac tttaacagat aatataccag cttctatttt
     3841 aagtccagaa tattcaatag ataatggagt gacttttcaa ccatggaatg gaagtcttag
     3901 tataggcact ttagatgctg gagagataag aaatattata ataaggggaa tagtaagtca
     3961 aacagcaata ggtacaatta taaatacagc aacagtaagt tcaactacac ctgacccaaa
     4021 tccagataat aatacttcaa cagatgaaat agatataagt tcatcagcag atatttctgt
     4081 aataaaatta gcaaataaaa cagaagcatg tgtaggagag caaatagatt ttgtaatagt
     4141 agtatcaaat gcaggaccag aaaatgctca aaatgttact ttaatagaca atgtttcaga
     4201 taagcttaag aaagcagtat tttcacttga tagaggtgta agtttccaac catggactgg
     4261 aagtcttaat ataggtacac taccagctgg aactttaaga gtgattatat taagaggaat
     4321 tataaaatca acatgtcttg atagattaac aaatattgca gaagttactt caacaacacc
     4381 agattcaaac cttaacaata atatatcaag agtgcaagta gaaataaaac aatgttgttg
     4441 taatgattgc tgttgtaaca actgtagtaa ttgttgttgc aatgattgtg gttgtaaaga
     4501 ttgttgctgt aattgttgta aaaatgactt ttggtgttga ttttataaaa taattttgta
     4561 taccttaaat taaactgaaa taaaaaataa attcacatat gagtaaaata ttttataaat
     4621 attaataaat tttctatctt tgatatggac cacattcgta atactagtta ttaaatgaag
     4681 tggtcctttt taattcatta aaattattt
//
LOCUS       DAELJO010000251         4675 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELJO010000251 DAELJO010000000
VERSION     DAELJO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.253"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(1012..1209)
                     /locus_tag="KPB88_003555"
     CDS             complement(1012..1209)
                     /locus_tag="KPB88_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055790.1"
                     /translation="MQIKIMELLDKKGKTRYWLAKEIGIKQQSLNKIASNETVSIRFD
                     ILEKICTTLECTPNDVLGWDK"
     gene            1342..1650
                     /locus_tag="KPB88_003556"
     CDS             1342..1650
                     /locus_tag="KPB88_003556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055791.1"
                     /translation="MINKNILKEQQLNKIKVLKNKNDKNIKNNIFKMFNSDMEEMKSI
                     IKNELFFENIYQDELEFHMNKIKNIENIADKFYVQFMISGEEGFEITCLRTALANRSI
                     "
     gene            2323..2943
                     /locus_tag="KPB88_003557"
     CDS             2323..2943
                     /locus_tag="KPB88_003557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055792.1"
                     /translation="MNKKVEKPFVYKREYDLTKYDTKVLQKYNLEQAIYIYVGSSCAY
                     NMRARSSKWRYHIRKNNGSICYTIRNFIHNLELFYKRELELPDNIIDDKLYYSNVVEF
                     EEFETLEKAREIESTIISQYQFLDSVNYMLKQKIILLSNNDSALCMVKNGNQVSLKIK
                     NKYIEKYKNKPIMRYHIDCQFNTDGSVKSITQEFEPILELSNKKIL"
     gene            3162..>4675
                     /locus_tag="KPB88_003558"
     CDS             3162..>4675
                     /locus_tag="KPB88_003558"
                     /inference="COORDINATES: protein motif:HMM:NF020291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG0055793.1"
                     /translation="MKGKDIQEKLITYFKFIFPKLETDEKYRAYMLVENSKTNFTRTF
                     KLNTYHELVEVVNKYNSKANIYISTCLYDNTKDKTKENILSKSVIVVDVDNADFDISE
                     IYKLAKINQVFIHLVVRSGRGWHLYFKLDKEYSNVKQLEKASKHLCELFGGDKNSLDV
                     GHVYRVPFTKNLKYDNTWSSIVNTINKTFPHTLDKLINHVPIKSSLSGNTELQVDNVE
                     QLSCFKNLVKYGISKGFRNKAMIAISNTCKYIGLSEVEALQYAYEFNSNCEVPQRKTE
                     VVKVVKSIYNNSSLIKPCKDNLNKKLCNSHCMGRLITVDDVLNKIDENIDNDIQVAFS
                     NKLIGNIKTYINKDGKKEKGTMLSVLSGAEILIISELKTFRNEIYSVDDLIDTTNLSK
                     PTIIKSLKRLEEFNIINSTKQHFNRSSKPTTIYFYNDECEQKYKEIHSIGRGLFIIRR
                     NKLITDNDMKVYIALKYLINKRMSTTQNDIELISGVTRNNINRSIKNLIEQDLL"
BASE COUNT         1914 a          468 c          709 g         1584 t
ORIGIN      
        1 aaaaaaacaa gttcaaagta caaatggaac atttaatatt tatacattaa aattatagaa
       61 aataaaaagc acttacaatt aatttgtagg tgctttttta aaacaagtta aaaatcaaaa
      121 atagtattag gaggcattgg aaatgctaga ttctaaaata gaaaagtaaa aaattgatgc
      181 atagtccaaa atacttcaaa ctattgaaat atacatattt tttgatgaga aaagtaaaaa
      241 attgatgcac tatctaaact tatctatatc tcttcttcct ttgttaggga ttaccttagt
      301 tacaatacag aggatatgct tatattaagt gagagtaata acccgtgttt aatatagtta
      361 ctagaattat agttactata gtttcgtatc tgtggcacaa taatagaata actaaaataa
      421 tgttagatgt ttacatgtac tttagttagg aactcgaatt ttacgagttg taatatctaa
      481 cactctaaat aatagctata gatattaaac gtgtacttga aacggacact tgaatatgtt
      541 tagaccactt gtgggagaaa gtataattgc cacgtgtggc taacacgcac gcgttagata
      601 taaattagtt aaatcctaat atgtggtata ccacttgaat atacattgtt gtgcaatatt
      661 gcaaatagta atagtcccaa caagttgggg aagtaacaaa tgaattttta cgttggttaa
      721 ggtgtaccca ttttggatac accctctata atttagttat gcaatatatg tttatcccaa
      781 atagtgatat taaaatactt gttttattct aatttaacaa caaaaccctt gtatgctagt
      841 tattgcaatg tttacattag ttttttatat gtcagtaagt taagacataa aactaagtat
      901 tttatgccat atttatatat atattagtag ttgcaatgtt tttataccta tcataggtat
      961 tgatttaaag ttaatttgtc agttacatgt cagtatattt gccataatag attatttatc
     1021 ccaacccaat acgtcatttg gagtacattc taaagttgta caaatttttt ctaatatatc
     1081 aaatcttatg gatacagttt catttgatgc tatcttattt aaactttgtt gtttaatacc
     1141 tatttcttta gccaaccaat atctcgtttt acctttttta tctaaaagct ccattatctt
     1201 aatttgcata aaatcctcct ccttaaatta ttgttaagag taagtatagt ttagaaggtt
     1261 ctgtttaaca ataatgtcta ttgacggtac ttacgctaca atggtattat gtttatatat
     1321 ttttgaagaa gggataaaaa gatgattaat aaaaacattt tgaaagaaca gcaactaaac
     1381 aaaattaaag tattaaaaaa taagaatgat aaaaatataa aaaacaatat atttaaaatg
     1441 tttaatagtg atatggagga aatgaaaagc attattaaaa atgagttgtt ttttgaaaat
     1501 atctatcaag atgaattgga atttcatatg aataaaatta agaacataga aaatatagca
     1561 gataagtttt atgtgcaatt tatgataagt ggagaagaag gttttgaaat aacatgtctg
     1621 agaacagcac tagcaaatag gagtatatag taaaggttta tataatatga tgacctcata
     1681 caagcctgta agagccgttt aaaagctgtc ttagtgtaat taatccttga aaatttaaaa
     1741 tacgtttaaa tggcttgtta aatatggtgg tattactatt aaatataggg ggtagttaca
     1801 tagttacatg ttgttttaaa aaataaattt aactatagtt ctttcacttc tacattacaa
     1861 aaaaataaat tgagtgtgta tgtttttagg tcaaaatgat gtttaaatat tgaaaaatag
     1921 ggatatggtg tatgtaaaat ttagaatagt gtatgtaatt ttaggcataa aaatatgtga
     1981 ttaaacctta aaaatcagga atatagtgta tgttaaaaaa gattgacata cgtgttaaaa
     2041 aaagttataa ttaaggatta gatatatatt gacatgattt attttctatg ttattatatg
     2101 tttagattaa aacatatcaa attaccatat ctatttatat attgactatt tgttaatatg
     2161 taacatgttt gtatttcaat gtaaatacaa tcttttatat cctgtgtgag ggtataccat
     2221 taaaagtatc ttatattcaa caaattaaaa aaatttaata ggaggtgtag tgtaaaagga
     2281 tttttagtta ctaagtttaa ataaaaaata ggaggacact tattgaataa aaaagtagaa
     2341 aaaccattcg tgtacaagag ggaatatgac ttaacaaaat atgatactaa agttctacaa
     2401 aagtataatt tagaacaagc tatatatatt tatgttggca gtagctgtgc atataacatg
     2461 agagctagaa gtagtaaatg gaggtatcat ataagaaaaa ataatggttc tatatgctat
     2521 accattagaa acttcataca taacttagaa ttgttttata aaagggaatt agagttgcca
     2581 gataacatta ttgatgataa gttatattat agtaatgtag ttgagtttga agaatttgaa
     2641 acattggaaa aagctaggga gatagaaagt actattataa gtcaatatca atttttagat
     2701 tctgtaaatt atatgttaaa acaaaaaata atcttgttaa gtaataatga tagtgcatta
     2761 tgtatggtta aaaatggaaa tcaagtatct ttaaaaataa aaaataaata catagaaaaa
     2821 tataagaaca agccaataat gagataccat atcgattgtc aatttaatac agatggaagt
     2881 gtcaaaagta ttacacagga atttgaaccc atattagaat taagcaataa aaaaatactc
     2941 taagccgaca agcagagtat ttagtataaa aaagatttat ttcaacaaca acactattga
     3001 tatagtgtga atgagttagc gttcattcaa gttgtttatt gactatattt aaaattacat
     3061 atattaataa gattttaaac ttatgataat ttaatgtaat ttaatgatat tattaattag
     3121 acattatgtc aagagtgttg ttctgaagaa aggagcaaca ggtgaaagga aaagatatac
     3181 aagaaaaatt gataacgtac tttaaattta tatttccaaa acttgaaact gatgaaaagt
     3241 atagagcata tatgcttgta gaaaattcaa agactaactt tacaagaaca tttaaactta
     3301 acacttatca tgagttagta gaagttgtca ataaatataa ctcaaaggca aatatataca
     3361 tatcaacttg tttatatgat aatacaaaag acaaaacaaa agagaatata ctatcaaaat
     3421 cagtaattgt agtagatgta gacaatgctg attttgacat atcagaaata tataaattgg
     3481 ctaaaataaa tcaagtattt atacatttag tagtaagaag tggacgaggt tggcacttat
     3541 actttaagtt agataaggaa tattcaaatg taaaacaatt agagaaggct tctaaacact
     3601 tatgcgaatt atttggtggt gacaaaaatt cattagatgt tggacatgta tatagagtcc
     3661 catttactaa aaatttaaag tatgataata cttggtcaag tattgtaaat actattaata
     3721 aaacatttcc acatacactt gataaattaa taaatcatgt accaattaaa tcatcactta
     3781 gtggaaacac agaattacaa gtggacaatg tagaacaatt aagttgtttt aaaaatcttg
     3841 taaaatatgg gataagtaaa gggtttagga ataaagctat gattgctata tcaaacacat
     3901 gtaaatatat tggtttaagt gaagttgaag cattgcaata tgcttatgaa tttaattcaa
     3961 attgtgaagt tccacaacgt aagactgaag tagttaaggt tgtaaaaagt atatataata
     4021 attctagttt aattaaacca tgtaaagata atttgaataa aaaattatgt aatagtcatt
     4081 gtatgggaag gttgattact gttgatgatg tacttaataa gattgatgaa aatatagata
     4141 atgatataca agttgcattt agtaataagt taattggtaa cattaaaact tatattaata
     4201 aagacggaaa aaaggagaag ggaactatgt taagtgtttt gagtggtgct gaaatattaa
     4261 taatatcgga gctaaaaaca tttagaaatg aaatttattc agttgatgac ttgatagata
     4321 caactaattt aagtaaacca acaataatta aatcattaaa aagacttgaa gaatttaata
     4381 taatcaacag tactaaacaa cattttaata gaagttcaaa acctacaact atatattttt
     4441 ataatgatga atgtgaacaa aagtataaag aaatacattc aatcggtaga ggtttattta
     4501 taattagacg caataaatta ataactgata atgatatgaa ggtttatata gcactaaaat
     4561 atcttatcaa taaaagaatg agtacaacac aaaatgatat tgaacttatt agtggggtaa
     4621 ctagaaataa tatcaatagg tctattaaaa acttgataga acaagattta ttaag
//
LOCUS       DAELJO010000252         4641 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELJO010000252 DAELJO010000000
VERSION     DAELJO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.254"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..519
                     /locus_tag="KPB88_003559"
     CDS             <1..519
                     /locus_tag="KPB88_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055794.1"
                     /translation="NFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYL
                     VELQQSKILQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNK
                     IVSIEIGTLFFIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLL
                     KKIYTRKVWFFD"
     gene            638..2029
                     /locus_tag="KPB88_003560"
     CDS             638..2029
                     /locus_tag="KPB88_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0055795.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2136..3176
                     /locus_tag="KPB88_003561"
     CDS             2136..3176
                     /locus_tag="KPB88_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0055796.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3665..4318
                     /locus_tag="KPB88_003562"
     CDS             3665..4318
                     /locus_tag="KPB88_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0055797.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            4394..4603
                     /locus_tag="KPB88_003563"
     CDS             4394..4603
                     /locus_tag="KPB88_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055798.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         1882 a          447 c          800 g         1512 t
ORIGIN      
        1 aattttcatg gatttgatta taaaaactgg caaagcagtg caaagataac tgaaaattta
       61 aaagaaaaac attttaattt ggatagagaa tataacaggt catatttttt aacattacca
      121 gatatgtatt tagtagagtt acaacaaagt aaaattcttc aatttatggg tgtttttata
      181 ggagtgatat tatttatagc agtttttagt tttttatact tcagattata tacagacgag
      241 atagaagata atgtaaagta caaaaaacta tcaaaaatag gtctatcttt taaaaatatg
      301 aataagattg ttagtataga gataggtaca ttgtttttta taccgtattt tatagcaatt
      361 ataaatagca taatttcttt actgttttta aatcaagtat taaataatag ttttaagtta
      421 gaaaatttgg gtatattact actgatatca tctatatatt cactttattt ttacttatta
      481 aaaaaaattt atactagaaa agtttggttt tttgattaaa gtggtatata atagatatat
      541 aaattaatat ttaaaaagta gttaatatta ggggagggca tttgcacctc ccttgtaata
      601 tgtggaaaat tgaatattag aagaaggagg agacttgatg gaaaatcaac aattattagg
      661 aacagaacga attagtaaat tactactaaa atattctata ccagctataa taggtatgtt
      721 ggtaaatagt ttatataatg tagtagatag aatttttatt ggaaatatac caggggttgg
      781 accacttgca ataacaggtc ttggtgtaac aatgccaata atgacaataa tacttgcatt
      841 tgggatgttg ataggtatag gaactactac taccatatca ataaaacttg ggcaaggtaa
      901 agtagaagag gcaagaaaac ttataggaaa tgcaatgacg ttatctgtaa taacaggaat
      961 tatcataatg atacttggaa tattatttgc aaataaaata cttacattgt ttggagcaag
     1021 tgaaaatact ttaatatatg caaaatcata tatcaatatt atattattag gtacagtagt
     1081 aaatcttctt tccttttctt taaaccactc aataagagct gatggtagtc ctaaaatttc
     1141 agcaggaata atgatagtag gatgtttaac taatatagtt ttagactgga tattaatatt
     1201 tggattcaat ttgggaatac aaggagctgc aattgctaca gtaacttctc aggctcttac
     1261 agctatttta actatagggt attatataag tggaaaatca aacttacgat tcagtaaatc
     1321 taatctaaaa ttagataaaa agctaataaa agcagtgttt gcaataggta tgtcgccatt
     1381 tgctatgcaa cttgcagcaa gtttagttca agtaatatca aatattgcac ttaaaactca
     1441 tggtggagat ttagctatag gggctatggc tactatttct tctattgcaa tggtattttt
     1501 gatgcctatc tttggaataa atcaaggagc gcaacctatt ataggtttca attatggagc
     1561 tgaaaaatat gatagggtta aaaaagcata tttaggttca ttagtagtag ctactataat
     1621 tttatgtatg ggtatggtag tagttatgct tttcccagaa gctattatag gtatatttaa
     1681 taaagaccct gaacttatga atatatctgt taatggatta agaatatact tacttatgtt
     1741 gccaattgta ggcttatcag ttacaggaac aaactttata caatctatag gaaaagcaaa
     1801 aatggctatg ttattgagcc ttttaagaca agtaatttta ttgataccag cagtattaat
     1861 tcttcctaca ttcttaggct tacaaggtgt atggacagct caaccagtgt ctgattttat
     1921 agctacagta attacaggta tagttgtatt tagagaatta aaaagatata ctcctaaaac
     1981 tgaaaaatta aatgaaaatg aaagactaaa cgagataact actgagtaaa aatataaatt
     2041 cagataattg agcaagaaat tgcaaaaaat acgattatat gataaaataa aaccgctgtc
     2101 aaggataaga gaaattctaa ttatggagtg attttatgga atttacaaag aaaagcaaat
     2161 taaaaaatat gtatgtaaac atgaatgtag ctagaatgtt gtctaagata aatgagtata
     2221 agggtagaca actgctttac aaaaaacaac caaaagaaat tttagaaaac ttagaaaaaa
     2281 aatctttagt agattgttct gaatctacat acattggaaa tcaaagagat agtagtaatt
     2341 ttaatctaga aaagttgata agtaatgaag ttacacctag aagtagagag gaattgagta
     2401 ttgttgaata cagagatgtg gtaaaaacta ttaacagtgc ttatgagagt attcccatca
     2461 gttcacaaac gattcttgag cttcatggat acttatataa attttcatct actagaggag
     2521 gaagttataa gtcagataat gattttattg aacacaattt aaaacctagc gaatttataa
     2581 gtactgattc aagtgtcaat aaagttgaaa aagctgtaga agaaatatgt gaagcttata
     2641 atacattgat agaggaagat gaaattgata tattaatatt aatttctgca tttgtgttag
     2701 atttcatatt gatacatcca ttcaaagaag gtaatataaa aatggcgaga gtacttatcc
     2761 tattgttact aaataaaaat gggtatgaag ttggaagata tataagttta ggaaagatat
     2821 ttgatgatag ttcatatgaa tattatagta atttaaatta tttgaaagcc tcaataggta
     2881 gtgaaaaagc agatatgaat gcatggattg agtatttttt agagacgata ttaactgcat
     2941 atgagaagct ggatgatagt ttaaatatat ctgataagaa aagacaaaca aaaactagta
     3001 gaatagagaa aataataaat tctacccttg gatattttac aaaagaagat ataagagatt
     3061 tatgtcctga cattccagaa cctactataa atagagtttt taataatcta agaaaacaag
     3121 ataagataga agttgttgca agaggaagaa gtgcaaaatg gaagaaaaaa tattaaaaaa
     3181 tgtttagata actaaaatgg tatagtgttt tacactatac catttttaat gtcattagca
     3241 tgagcaataa tttaacaact taagtaatat caattttatt tattgattat ttttgaactg
     3301 aaaaatggcg ttatatggta tacctcagtg gtaattaatt ttggatgaga agatataata
     3361 ttacagaata tattaggagt atatgtagaa aaaatataat aaaagtgtga ggcttagtaa
     3421 ttaacaaatt atgtggcttc acactttttt ataaaataaa gatacttagc agaataatat
     3481 tttagttgat atgtttataa gatggtactt gtttgggtaa aagtataaat agtaaaaaag
     3541 tagaagtaat gtgtataaaa acataagtta cattctgtaa taatagttat tttataattc
     3601 aacaaaagaa tagaatgggt atatttgtga aataataata taaaataatc ttaggagtga
     3661 gcttatgaag aaaaaattat tatatataaa tgtaaattca aaacctgaag acttatcttc
     3721 aagtaaaact gtagcaagaa aatttataaa taaatttatg gaaaaaaata aagattttga
     3781 ggttgaagag atagaccttt ataaagaaca tataccaaga cttgagtatc agtactttga
     3841 aaaaagaaat agcatagtta gtgaagaaaa tgctaaaaat ttagacgata aggatagaaa
     3901 agaacttaca aagattagaa atctttgtga ccaatttgtg agtgcaagtg tatatgtaat
     3961 agcagcacct atgtggagtt tatcttttcc agcaccactt aaagagtata tagattgtat
     4021 aattcaagat ggaaaaacta tatcttttga aggcaatgat aaaccacaag gaattttaaa
     4081 tgatattgat agaagtatgg tatatataca atcttctggt ggacacatac catgggtgtt
     4141 gaagccagtt atgaataaag ggttaaatta tgtggaaagt attatgaaat ttataggaat
     4201 taaaaaattt gatgaattgt tagtagatgg aactggaact agtgaagaag aaagacaagc
     4261 tgcaatagaa aaagcatctg aaaaaataga tggtataata gatgggatga aattttaatt
     4321 ataatatttt attaaaaata taaaggaaaa acctctttta atgtagaaac ttatattgaa
     4381 ggaggcgaga atcatgcgag aagaaaaaag taatgaaaag tatgattgtt attggtgtaa
     4441 tcaagagaat aatttctgtg tagaaataaa agataatata gtcatgatag atgatggcac
     4501 tggtacgtta aaacaagccg ttttcatagg gtataaacaa atccaaatta atttaaattg
     4561 ttcacattgt caaaacttaa atagaataaa attaaatttg tagaatttta aaatggaaat
     4621 tattgaagga attaaaaata g
//
LOCUS       DAELJO010000253         4626 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELJO010000253 DAELJO010000000
VERSION     DAELJO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.255"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            134..2545
                     /locus_tag="KPB88_003564"
     CDS             134..2545
                     /locus_tag="KPB88_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0055799.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2842..4419
                     /locus_tag="KPB88_003565"
     CDS             2842..4419
                     /locus_tag="KPB88_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG0055800.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         1876 a          528 c          847 g         1375 t
ORIGIN      
        1 aatcaataaa tatgcagtat aaaatagtat tttatatatg tataattata attcctattt
       61 ttataaaaat aaaatatgac ctagatttag aaatctgata taataaaaat taaatacata
      121 caagggggta aatatgagaa agtataaatt aaaaaaattg tcaaagctac tggtgttgtt
      181 gacagtttgt tttttaattg tgtcaacaat acctgtctca gcagaaaaaa ataaaattct
      241 gactggagta gaaactgcag agtattctga atcatatctt caatacttgg aagatgtaaa
      301 aaatggagat atagcaaaat ataatggagt aataccaact ccttatgaaa tggaaggaac
      361 aacacttaaa actaatgtaa gaagtagtct tccagcatca tataaatcaa gtgtatcata
      421 tgatccaaga aaattagatt taactacacc agcaaaagac caaggaaaac ttaatacatg
      481 ttgggctttt tctggaatgt caacactaga agcatatttg aaattaaaag gttatggaac
      541 ttatgatctt tcagaagagc attttagatg gtggtcaact ggaggaatac atggatggaa
      601 cttaactgat atgacaggta gttcgaatgt aactgcaatt ggatatctta cagcctgggc
      661 aggacctaag cttgaaaaag acattccata taattttaaa tcagaagatg aaggagcaac
      721 taggcctcaa aatatggata ctgcacctac acaatttaat gtaacagatg ttgtaagaat
      781 tagtaaagat aaaacttctg ttaaaaatgc aataatgcaa tatggtgcag tgacatcagg
      841 atatgcacac tattcagcct acttaagtga tgatgaaaat tcgtataatt gtaatgataa
      901 aagtgaacct ttaaatcatt ctgtttcaat tgtaggatgg gatgataatt attcaaaaga
      961 taagttcaaa ccaagtgtta gaccagaatc aaatggagca tggttagtaa aaagtagttg
     1021 gggagaattt aactcagaaa aaggattttt ctggatttct tatgaagaca aaactctttt
     1081 aaaagataca gataactatg caatgaaatc agtatcaaaa ccagatagtg ataaaaaaat
     1141 gtaccaactt gaatatgctg gtcttagcaa gataatgtca aataaagtaa cagcagcaaa
     1201 tgtatttgat tttagcagag attctgaaaa acttgactct gttatgtttg aaacagattc
     1261 tgtaggagca aaatatgaag tatattatgc accagtagta aatggagttc ctcaaaacaa
     1321 ttcaatgaca aaacttgcaa gtggaacagt atcatattct ggatacataa atgtacctac
     1381 taattcttac agcttaccaa aaggtaaagg agcaatagta gtagttatag acaacacagc
     1441 aaatcctaat agagaaaaat caactttagc atatgaaact gatatagatg gatactatct
     1501 atatgaggct aaagcaaact taggtgaaag ctacatactt caaaacaata aatttgaaga
     1561 tataaataca tatagtgaat tttctccttg taactttgtt ataaaagcta taacaaaaac
     1621 atcttctgga caagctactt caggagaatc tttaactgga gcagatagat atgaaacagc
     1681 agttaaagtt agtcaaaaag gatggacttc ttcacaaaat gcagtattag taaatggaga
     1741 tgcaatagtg gatgctttaa cagctacacc atttacagca gcaattgact ctccaattct
     1801 tttgacagga aaagataact tagattcaaa aactaaggca gagttacaaa gattaggaac
     1861 taaaaaagtt tatctaatag gtggagaaaa ttctttaagc aagaatgtac aaactcaact
     1921 tagtaatatg ggtatatcag tagaaagaat ttcaggtagt gatagataca agaccagtat
     1981 atctctagct caaaagttaa atagtataaa atctgtttca caagttgcag tggcaaatgg
     2041 tgtaaatgga cttgcagatg caataagtgt tggtgcagca gctgctgata acaatatgcc
     2101 aataatactt actaatgaaa aaagtgagtt gcaaggtgct gatgagtttt taaattcatc
     2161 taaaataact aagtcttata taattggtgg aacagctact ttatcatcaa atttagaaag
     2221 taagctttca aatccaacaa gacttgcagg aagtaataga aatgaaacta atgctaagat
     2281 aatagataaa ttctatccta gctcagattt gaaatatgct tttgttgtta aagatggttc
     2341 aaagagtcaa ggagacttga tagatggact agcagtagga gcattaggtg ctaaaactga
     2401 ttcaccagta gttttagttg gaaataagtt agatgaaagt caaaaaaatg tacttaagtc
     2461 taagaaaata gaaactccta ttagagttgg tggaaatgga aatgaaaatg cttttaatga
     2521 actaaatact cttttaggaa aatagtgaat ggaaagatag taaggatata cattttatta
     2581 ttaaaaaata ttcaatagaa gtttataaat ataaaataac aaaacggggt gtctcaaaat
     2641 gaacttttta gttcatgagg cactcttttt tgttgtttta tatttttatc atttaaataa
     2701 tgaaaaatag gcttgtctat atttttagat agttttctta ttattgatga aaaatgtaat
     2761 aattttaaac tgtaaaatag tgtaaagtta ttttataata taaatatagt aagattttaa
     2821 acaaaataag ggggaataat aatgaaagca ccaaaaacta ttttaacaat actgacaata
     2881 gcgcttactt taagtagcat ttctataata ccatcatatg cacttacaga ggaaaaatta
     2941 ataggtaatg gtagatatga aactgctgta aagataagtc aaaaggcata cagttctagt
     3001 cctaatgtag tgctagttaa tgacaattca ttagctgatg cactttcagc tacaccattt
     3061 gcaaaggcta aaggagcacc aatacttttg acagaaagtg ataagctaga tgataggact
     3121 gaaaaagaga taaaacgtct aggagctaaa gatatttatt tgataggagg tactgctgta
     3181 cttaataaag atatagagaa caagttaaaa agtaatggac ttaatgtaga aagaattaat
     3241 ggtaaggata gatatgaaac ttcactgatt ttggctaata agcttaagga tataaaagat
     3301 attaaggaag ttgctgtagt aaatggtgaa aaaggattga gtgatgctgt tagcgttggg
     3361 gcaccagcag ctcaaaataa gatgcctata attttgtcta gtcctaagga cggagtagaa
     3421 gcatttgata aatttataag ggatgagaaa gttataaaag catatgtaat tggaggtaca
     3481 aattctgttt caagagctgt agaaaagagt cttcctaatg cagaaagagt gagtgggaaa
     3541 gatagaaatg agactaatgc taaggtcata gaaaagtttt acactgatac aaatttaagt
     3601 aatttatatg taactaagga tggtagtaaa aatgagaatc aactaataga ctcgttagct
     3661 gttggagtat tggctgcaaa aaatgagtct cctgttgttt tggttggtaa taaattaaac
     3721 acaaaacaaa gggacatact tagtactaaa aaattaaata ctataacaca ggtaggtgga
     3781 aatggaaatg aagaagcttt tgatgaaatt aaaagtctac aagaaaaaac tgtgtttgaa
     3841 gcaaaaactg ttgaggaatt aactgatatg ataaatatag caagtcctaa tgatattata
     3901 aactttaaac caaaagagaa tactgtaaat gaagctttta gaatggtaac aaataaacca
     3961 ataactgtga atataaaggg tgattgttca aagactctta cagtagatat gcctaatggt
     4021 gaagtgaata actatgctac tttggtaaat gttatagtta gaaatattgg agaaggtgga
     4081 tttaataacc atgatacaat cactatattg tcagttcgtg acaaaaatgg tagagttata
     4141 gaaaatacta gaaattcaga tatagatacg ttgatgatat tagcttctgc taatgataca
     4201 aaattgatta atgatggata tataggaaaa cttatagata attcatctaa tagtgatata
     4261 actaataatg gtactataga taaaaaagtt aatcaggttg aagatttaga ggctaaagta
     4321 gattcaattg aaaaagctat agattctata agtcaaaaag tcaataaaat acaagatata
     4381 ctagataagc ttggatttct aaagaaattt cttagctaag aatatatata tttaagtgaa
     4441 gtgtttgttg taattgttct aaagttatgt agttaaacag gtttatatac aaatagattt
     4501 atagataaat atgtagttta gtatatattt gaataagctt tggggaggta acatggggat
     4561 tggaattgaa tgtaacaaaa aaataaataa aagtattgac aaaactagaa taaagaaaaa
     4621 taatct
//
LOCUS       DAELJO010000254         4599 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELJO010000254 DAELJO010000000
VERSION     DAELJO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.256"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            68..757
                     /locus_tag="KPB88_003566"
     CDS             68..757
                     /locus_tag="KPB88_003566"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG0055801.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            758..1330
                     /gene="yqeK"
                     /locus_tag="KPB88_003567"
     CDS             758..1330
                     /gene="yqeK"
                     /locus_tag="KPB88_003567"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG0055802.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            1447..1797
                     /gene="rsfS"
                     /locus_tag="KPB88_003568"
     CDS             1447..1797
                     /gene="rsfS"
                     /locus_tag="KPB88_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG0055803.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            2151..4571
                     /locus_tag="KPB88_003569"
     CDS             2151..4571
                     /locus_tag="KPB88_003569"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG0055804.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
BASE COUNT         1859 a          507 c          888 g         1345 t
ORIGIN      
        1 aattataagc tttaagaaat aaaaaataat ggtacaatag tattataaca ttaaaaggag
       61 acaaaatatg agaagtgaaa atctagttaa gatggcagag ttgaaaaata ctgaaaaact
      121 agagaaattt aatcgccata aaggcaaaat caaaataggt atattgggag gtacttttga
      181 ccctatacac tatgctcatt tagcaacagc agaatttatt agagacaagt atgacattga
      241 caaaataatc tttataccat caggaaatcc accacataaa ttatgtatca ctacagataa
      301 gtatgataga tataatatga cacttctagc aactgaaagc aacgaagatt ttttagtttc
      361 aaaagtagaa atcgaaagaa agaaaaggac ttatacaata gatacattaa aatacttaaa
      421 gaaaaaatac aaaaatgcag acatttattt tataacaggt gcagatgcaa tttgtagtgt
      481 tgaagagtgg aaagatgtaa agaaaaactt tgagttagct acttttatag ccgcaactag
      541 accaggaatt agtttgttaa ggtcacaaga gactatagaa aaactaacta agaagtataa
      601 tgctgacatt ataacagttt atgttccatc actggatata tcgtccacat atataaggga
      661 acagctaaat gaaggcaagt caatacgtta cttagtaccc gaaaatgtag aaaattattt
      721 atatgaaaat aaactatatc aatatggaga tgattaaatg aacttagaaa atattaacca
      781 aagactaaat caaatgctac cagtggggag gcttagccat tcaaaaaatg ttgcaaaatg
      841 tgctgaaaaa ttatgtgaga tttatggatg tgacaaagaa aaagcttact tagcaggaat
      901 gattcatgat tgtgcaaagt atttaagtga caaggaaata gaggattatg taaataagta
      961 tgaaatatat ttagacccaa tagaggatgg aaatcgttcc ttgtctcata gtgtaattgg
     1021 ggcgtacata tgcgagtatg aatttgaagt agaagatgaa gatattataa atgcaatcaa
     1081 atatcataca acagggagag aggacatgtc tcttttagaa aaaataattt acatagctga
     1141 tttaattgaa gaaggaagaa aattcccagt tgtagataca ttaagagaat tagcatatgg
     1201 agggaagctt gatgaagcac ttttaacttc ttttaataat actttaatgt ttgtcatcaa
     1261 taaaaaagaa gagatacatc caagaacagt tatggcaaga aactatctaa ttaaagaaaa
     1321 gttattataa aaaagctata taaaggcaaa catattaata aatttaaatt tgactatgtt
     1381 acaaaatcgt attaatatgt tacaataact aagtgtattt taattaggaa agtgaggaat
     1441 ttttatatga cagtggaaca aatgacaaaa atagcatatg atgccataga agataaactg
     1501 ggtcaagata cagttataat aaatatagga aaagtttcta gtttgtgtga ttattttatt
     1561 attactacag catcttctca gagacaggta aaagctattg ctgacaatgt agaagatgaa
     1621 ttagcaaaac taggattaga gccaagaggt aaagaaggac aaggaactca aacttgggta
     1681 ctccttgatt atggagacat aatggttcat gtatttaatg aggaaaatag agggttttat
     1741 aatttagaaa aattatggaa agatgcacct tatatagata ttgacacatt agcataaaca
     1801 tgataatata tgttattata attagaaaat ttcaaaaatt atattaaatt gaatagacta
     1861 gagcagtaaa agattatatt ttttcagaga gttagtggaa ggtgagaact aatattatat
     1921 aactttgaac ttactagatt aaaatctatt ctccatggct ggattaaaag ctatctaaga
     1981 gagtctatat atgtagtagg ctaattaggg tggtaccgcg aagatttatc ctcgtcccta
     2041 aacgtaagtt tagtgacgag gattttttat tttcaaagaa ttatttggaa attataagta
     2101 aataatacta aataaggtag ttatacacaa ttaaaaatag gaggcaaaat atgagcgtat
     2161 ataattttaa agaagtagaa tcgaaatggc aaaaaatttg gaaagataat aatcagtata
     2221 aaatggatac tgcacaaact gaaaaaccta attattacac attagaaatg ttcccttatc
     2281 catctggaaa aatacacatg ggacatgtta gaaattattc tataggtgat gttgtcgcaa
     2341 gattcaaaaa aatggaagga tataatgtac ttcatccaat gggatgggat tcttttggat
     2401 taccagctga aaatgcagct ataaaacatg gaatacatcc acataaatgg acaatggaaa
     2461 atatagaaga aatgaaagag caattaaatt tattaggtat tagttatgat tgggataaag
     2521 aagttgcaac ttcaactcca gaatactata gatttacaca agaaatattt ttgaagtttt
     2581 tagagcatgg gcttgcatat aagaaaaaat catatgttaa ctggtgtcct tcttgtgaga
     2641 ctgttcttgc aaatgagcaa gttgttcaag gagcatgtga gagatgtaaa gcaacagtat
     2701 taaagaaaga tttagaacaa tggtatttta agactactga atttgcagaa gaattactta
     2761 atgacttaga tacattagat ggatggccag aaaaagttaa aacaatgcaa aaaaattgga
     2821 taggtaaaag tacaggagca gatttagttt ttgatataga tggaactgat aaatctatga
     2881 cagtatttac aactagacca gacacaactt atggagttac ttatatggta cttgctccag
     2941 agcatgaatt agttaaagaa ttggttgcag ggactgaata tgaagcagat gttgaagctt
     3001 ttgttcaaaa aatgcatact atgacagaaa tagaaagaac agcagctgat gtagaaaaag
     3061 aaggaatgtt tataggtaga tatgttataa atcctttaaa tggcaagaaa gttcctctat
     3121 ggatagctaa ctatgtatta gttgagtatg gaacaggtgc tataatggca gttccagcac
     3181 atgatgaaag agatagagat tttgctgaaa aatataattt agacataatt gatgtaataa
     3241 cagaagataa caaaatgata aattcagaag aatttaatgg attagatgct tcagaaggat
     3301 ttgaaggaat aatcgataag ttagaaaaag aaggtagagg aaagagaaca attaattata
     3361 gacttagaga ctggttagtt tcaagacaaa gatactgggg ttgtcctata cctgtggttt
     3421 attgtgatga atgtggaata gttccagtta aaaaggaaga tttaccagtt cttttaccaa
     3481 cagatgttga atttactgga aaaggggaat ctccacttac gacttcaaag caatttatgt
     3541 caacaacttg tcctcactgt ggaaaaccag ctagaaggga agttgatact atggatacat
     3601 tcgttgattc ttcttggtat ttcttaagat atgtggatag caataatgaa aatgaaccat
     3661 ttagtaagga attagttaat agatggcatc cagtagacca atatataggt ggagtagaac
     3721 atgctataat gcatttactt tatgcaagat ggtttgtaaa ggcatttaag agtatgggaa
     3781 tggtagactt caatgagcca tttaaaaact tacttactca aggaatggta cttatggatg
     3841 gttctaaaat gagtaagtct aaagggaata cagtatctcc tatggatata atagatgagt
     3901 atggagcaga tactgcaaga cttttcgtat tatttgcagc accaccagaa agagatttgg
     3961 actggtcaga gcaaggtgtt gatggatgct ttagattctt aaatagggtt tatagattgg
     4021 ttgacgagtt agcagatgtg gttaaaaagg atgtagagtt tggagaatta aattcccaag
     4081 ataaggatat gagatatact atacactcta cacttaaaaa agttactgct gatttaagtg
     4141 aaaaattcgg atttaatact gctatatcag ctcttatgga attaataaat gatatgtata
     4201 aatataaaga gttagataat ataaacgaag cagttataaa agaaggtgta caaactatag
     4261 taactataat tgcaccattt gcccctcatt taggtgaaga actatggact atgataggta
     4321 aagaaggaag tgtatttgat atagactggc ctaaatatga cgaaaaagct ttagtaaaag
     4381 atgaaataga agttgttgta caagttaatg gaaaagttag aggaaaatta actgttaact
     4441 caaatatatc taaagatgag atggaaaagg tagctttaga agatgaaaaa ataaaaggat
     4501 tagttgaagg taaaactata gttaaagttg tagctgtacc taaaaaatta gtaaatatag
     4561 ttgttaaata actcaaaaat ataaacaaaa atataaatt
//
LOCUS       DAELJO010000255         4590 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELJO010000255 DAELJO010000000
VERSION     DAELJO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.257"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            180..509
                     /locus_tag="KPB88_003570"
     CDS             180..509
                     /locus_tag="KPB88_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055805.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            513..1304
                     /locus_tag="KPB88_003571"
     CDS             513..1304
                     /locus_tag="KPB88_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055806.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            1868..2950
                     /locus_tag="KPB88_003572"
     CDS             1868..2950
                     /locus_tag="KPB88_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0055807.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEAVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(3162..4355)
                     /locus_tag="KPB88_003573"
     CDS             complement(3162..4355)
                     /locus_tag="KPB88_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0055808.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT         1723 a          593 c          716 g         1558 t
ORIGIN      
        1 taaaatttga taaatagctg tatattagta tttatagatt aaatattaat atacagctat
       61 ttattatttt tgagtctatt ttagaatata aaccttgcat tatagagtac tatgtattat
      121 tatagtaaat ataagtacta tacattatag agtagtaggt gaaaaggcgg tggtacataa
      181 tgcagttaaa taaagaagtg ttaaaaggtt atatcgatat attaattgtt tctatattag
      241 aaaagaaaga ttgttatgga tatgaaattg caaagcaagt aagagaaaga agtgagttcg
      301 aattaaaaga aggaacaatg tatcttgcac ttaaaagaat ggaatctaaa aatttaatta
      361 aatcttatta tagcaatgaa caaagttcag gtggaagaag aaaatattat aatttaacta
      421 atgaaggtaa agatttttta gaaataaaga aacaagaatg gagatttatt aaaaaggtta
      481 tgaatcaatt tttgggagag gtggattaat ttatgaaact aatagatgaa tatttagata
      541 aactatataa aaaatgtgac aataaaagta ctatagaatt aaaacaggaa atgagatgcc
      601 atcttataga atctgcaaat gaatttaaat tagaaggatt agatgaagaa gaagcatgta
      661 aaaaagcaat tgaaagattt gatgatggtg atgaaatgca gtatgaatta tgtaatataa
      721 taaaagaact atctttatca ttggataggc ataaatcaat tgtaatgggg tttaaaaaag
      781 tattaggtta cataagtata attgcatttt taatttctgg atttatgtgg tattacaata
      841 atagtttaca acataatatg tataatttag gaaaagaact tgatggagaa ataaaacaat
      901 tggcagaaag acatgatatg acaaagattg gtgaatataa gttagaactt gaaaaaatac
      961 ttgataaaga taagtatagt aaagtcaaag ctcttagact atatgtaata gatatgaaag
     1021 atggaaacac taatttaagt tcttctggat tgaatgctaa tatggtatat gagagggaag
     1081 cagattataa taacataagt aattttatac agcatttagg atataatgga aaagattttt
     1141 tagacaagaa tgggaatata gtaaatccag atattttctt agaatatttc ttttattttg
     1201 aatcggagat gttaattcca gtagcatttg catttggatt attatgtata atagcgtatt
     1261 ttatattaag atttaaaatt tctttaataa aaaataataa ttaaataatt caccaaataa
     1321 tcttaatttg tagagtaaat aagatgtttc aaaataaaat ttgaatcatc ttatttttta
     1381 ttgtttgtta tgctcttttc ttttatattt ctccatagtg gattgtatct atacataatc
     1441 acatattttg atataattat actttccaaa aaatgtattg aatatacaaa ttataatctg
     1501 tataatgttg ttaggggaga atgtatgaag tgtatacaat tttaatttta aatgtattat
     1561 tgacaattct cctcactctc gccattacca ataattggaa aattgacata cctgttacag
     1621 accaacattt ttgaatatat taattattat cattttattg tgatgatatc aaaaatgcag
     1681 gtttgtgagt tggattgtat acgaactata taggaaatat agaacagatt ttccaagaaa
     1741 tctaaatatt ctatgaccac gtgaattgct atgcatacat gttgataatc catattttaa
     1801 atctaaaatg aacttaatta atggtaggga attgaaagta aaatatatta taaaggagaa
     1861 tgctaatatg aaaaaaatca ggaaattagc tactaaacta ttgattgcaa tgatggtttt
     1921 aacctcatgt atgagtgtaa cagtttatgg tatgacagct aaagatgtta ctgcaaagac
     1981 accgaaaagc tatgtagctg gaaccaacag tatatatggt ccaaagttat cacaagctca
     2041 acttaattcg gtagcacaag caacagcaga ctttatgaac aagaaaatta caaagaacat
     2101 gaccacagat gctaagatta gggttgctta taatcacatt aaaaacaaca caacatatgt
     2161 taactggaac gcagcagagg gagccaatac tgcttataca ttggttacta aaaaaggtgc
     2221 ttgttctgga atgtgtagga gcttgaaagc tttatgtgat gctatgggaa ttgaatgtta
     2281 ttatgtacat agtacaacaa atgaccatca gtggaatctt attcgttttg aggatggacg
     2341 tttgtatcat gtagatattg atgccaacaa atcagcagga gcagacatca tatacaagag
     2401 tcttactcac ccattaccat ttgacaagaa agcatatcca gctgttggcg aagcagtaaa
     2461 gacacagaat ccaacgccaa ctgttaagaa acctacgcca gctaagcctt ctgcgccagt
     2521 tggacctggg aatcacacta tgatagcaga tcaacaaggg gcaatacttg atgaagttta
     2581 tggaaataat ctacgtccaa aaattgaaat gtatgaggga gttccgtgta gtttaatcaa
     2641 ctcatcaata aatacaatta agtttacaag caaaaattca aatttagacc catcaaagca
     2701 tacagttact gtaatagggt ttatgaatac accatatggt atggttgcat tagctccaga
     2761 tggtgtacct gatgagtatc cttttgaata tgataagcca ttttctttag attttactgg
     2821 taagaacctt tctgcagcag aattatcaaa aaagtgtaaa gaaaataatg taattattaa
     2881 agtggaaatt ggtgaaacgg catctaattc tggaagaggg atggtagcat acgctagata
     2941 tgtgaattaa tacaaatata ctagtgtaca aatagaacat attataaata atgcaccctc
     3001 gcacaaaggg tgcatttatc attatatcag tcattttaga aaatgaaaaa ggagttgaac
     3061 ttaaaattag attagaaaaa ttaattttga gacaactcca ttttaattta tttttaaata
     3121 taaaatcatt tattttaaat aatattatgt gtgattaatg gctatttaac taattttgca
     3181 aattctgcac caaatgccat acactctttt aatgtctctt cactagggaa gaattttttc
     3241 ttaagagatt ctacaggcat tttgaatgac atagatttaa gtaaagtctc agccatagtt
     3301 ataccttcac cactccaacc aaatgaacca aatactcctg ctatcttgcc ttggttagcc
     3361 attgggtcta taacagagaa taaatcccac ataggtttaa ccatagtttt gtttatagtt
     3421 ggagagccta gtaatattac ttttgacact actaatgtat cgtgcatttc tgttaatgtc
     3481 atatcttcta aatcatataa ttcagccttt gcaccttctt tatcaagtcc ttcttttatc
     3541 tttttagcca tctctaaagt attactataa gctgataagt agaatataga aacttgattt
     3601 tgattagttg tattaacagc ttctgtagac cactcaacat atcttttaac agcagccatt
     3661 gggtctttag taagcattgg accatgagaa gtcaatatag tatcaaattc tatgtttagt
     3721 cctaccacct tatctacagc acttaaaaca tgtttagcaa atggcttaac tatacaatca
     3781 tagtagtgtt tagctgattt tagataatct tcactattca ctacctctgc atcaacactt
     3841 gcaaagtgac aaccaaatgc atcacaagtt aaaagagtct tgtcctcctc aatgtaagta
     3901 aacattgtat caacccaatg taagaatgga gcagttataa atctaagatt tcttttaccg
     3961 atatttaaaa tttcaccatc ttttattaca tgacagttaa aaggtctatt tatttgacca
     4021 tctaagtata gtttagcagc tttagtacaa taaacttcta tattaggatt tatatctaat
     4081 aagtatttta aactacctgc atggtctggt tctgtgtgat gaattacaac atagtcaatt
     4141 ttagctatat cagtaacctc tgataaatta cttaaaaact catctttaaa atttgccttt
     4201 acagtgtcaa ataaaaccgt tttttcgtcc ttgattaaat aagagttgta agttgtccca
     4261 aactcagttt ccattattat gtcgaatact tttaaacctt catctactac gccagtaaag
     4321 tagatatctt tttttacttc aaaaacttta ctcattttta accctcctaa aatttatcat
     4381 tatataagta acatacccca ttttgaggtg aatgaaacaa aagatgtcat gataagtcat
     4441 gtaaaacaat agttttatgt ttataatata tcatactttg taaaaaaatt gtatttcaat
     4501 aaaaatttaa tagaaaatat tcttaaatat ttttagctat tctattttgt gaataatatg
     4561 tattttaaat tcaatatttt agttatattt
//
LOCUS       DAELJO010000256         4558 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELJO010000256 DAELJO010000000
VERSION     DAELJO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.258"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..738
                     /locus_tag="KPB88_003574"
     CDS             <1..738
                     /locus_tag="KPB88_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0055809.1"
                     /translation="FIEFQNELYIIEVNPRASRTVPYISKVSGVPIVDLATKCMLGAK
                     LKDLGYGIGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVLGVGENLEEALY
                     KGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGI
                     EADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEFSTEIMTS
                     LDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            996..2045
                     /gene="carA"
                     /locus_tag="KPB88_003575"
     CDS             996..2045
                     /gene="carA"
                     /locus_tag="KPB88_003575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0055810.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            2238..>4558
                     /gene="carB"
                     /locus_tag="KPB88_003576"
     CDS             2238..>4558
                     /gene="carB"
                     /locus_tag="KPB88_003576"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG0055811.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         1870 a          461 c          918 g         1309 t
ORIGIN      
        1 tttatagaat ttcaaaatga attatatata attgaggtta accctagagc tagtagaaca
       61 gttccgtata taagtaaagt aagtggagta ccaatagttg acttggctac taagtgtatg
      121 ttaggggcta aattaaagga tttaggatat ggtataggag tttacaaaga gccaaaatta
      181 gtatcagtaa aagtaccagt attctcaatg tctaaattat ctaaagttga agtaagtcta
      241 ggacctgaaa tgaagtctac aggagaagtt ctaggtgtag gagaaaattt agaagaagcc
      301 ttatataaag ggttcttggc agcaggtaga catatgtctg atgaaagagg agtagtactt
      361 gctactgtaa ataatcatga taaagatgag tttatagaga tagcaaaaga tatgaaagaa
      421 ttaggatata cttttgttgc tacagaagga acagcgaaga gtttaagaga aaatggaata
      481 gaggctgata tagtaaatag agtagaagaa tctagaccaa acatattaga tgctataaga
      541 aataaacaag ttgatatagt aataaataca ccaactaaag gaaatgactc tacaagagat
      601 gggtttaaga taagaagaac agctattgaa ttttctactg aaataatgac atctctggat
      661 acactaaaag ctttagtaga agttaagaag aaacatttaa ataaagatga gttaaaagtt
      721 tacaatatag ctgaatagaa attaagttga ttgtatagtt taagaattta attattaaat
      781 aaaaaaaaag aggttatttt ttctaggagt ttttactttt gagataactt ctttttttta
      841 agaaatttgt agtataatag aaaaagataa aaaataaatg taaataagta taaaactaga
      901 atgtaaaagt gaaaaataga tatagatatt taaaagctta tatttagtta atgttcttta
      961 gtttgtctaa agagaattat aggaggtact gtagtatgaa agcaaggtta atattagaag
     1021 atggaacagt ttttatagga aaggcatttg gatatttaga agaaagtgta ggagaagtag
     1081 tatttaatac ttcgatgata ggatatggtg aagttttaac agacccttct tactatggtc
     1141 aaatagttac tatgacttat cctcttgtag gaaattatgg tataaattta tcatcagcag
     1201 agtctgaaaa agttcaagta aagggattca tagtaaggga gaaaagtgat tctccaagta
     1261 attttagatg tgaaattgat atagaccaat atttaaagca aaataaagtt atcggacttg
     1321 aaggaataga tacaagagct ttgactaaga tacttagaaa taatggaaca atgaaaggta
     1381 taattacatt agaagacagt aaattagaag atgttaaaca taaattggat aagttttcaa
     1441 atacagaagc agttagaact gttacaagaa aagaagtaga gcatataaaa ggaaatggtc
     1501 caaaagtggc tgttatggat tttggagtaa aaagaaatat attaagatca tttatagctc
     1561 gtggatgtga tataacaata tttccagcta caacatctcc agaagatgta ttgagtataa
     1621 accctgactt aatattcttg tcaaatggac ctggagaccc agaagactta gaagatgtta
     1681 tagaaaatat aaaagctctt attggtaaaa aacctatagt tggaatatgc ttaggacatc
     1741 aacttttagc tttagctctt ggaggaaaaa ctgctaaact taaatttggg catagaggtg
     1801 gaaatcatcc agttaaagat ttggaagaag gaaaagtatt tataacttct caaaatcatg
     1861 gttattatgt ttcagaagtt cctgaacaaa tgaaagtgac acatataaat ttaaatgata
     1921 atactgttga aggaatgcga catgagaagc tagatgtata cagtgttcaa taccatcctg
     1981 aagcttgccc aggaccaaaa gataatgact atatttttga taaattttta gaattagtaa
     2041 aataaataac attagaatag ctaactaaaa aacaagaata acttaaatca aaaagtttgg
     2101 tttaattaac atagatgtat taaatagatg tattgatgag taaatttgaa cttaagataa
     2161 aataaagtaa ttaaagttga ttgtatagat gatgaagata agaactgtga aaattgttta
     2221 gataaggaga aagagttatg cctaaattag atagtataaa gaaaacttta gtattagggt
     2281 cagggccaat aataataggt caagctgcag agtttgacta ttcaggaaca caggcctgcc
     2341 aagcattaaa agaagaggga attgaagttg tattagtaaa tagtaaccca gctactataa
     2401 tgactgataa agaaattgca gataaaatat atatagaacc attaacaata gaatttatag
     2461 aaaaaataat agaaaaagaa agaccagaca gtttacttgc aggtatgggt ggtcagacag
     2521 gattaaactt agctgtagaa ttacatgatg ctggtatatt agataaatat aatgtaaaag
     2581 ttataggaac atctatagaa tctataaaaa agggcgaaga tagagattta tttagagaag
     2641 taatgaaaga aataaatcaa ccagtaatag ttagtgatat agttacaaat ttagaagcag
     2701 gtcttgagtt tgccaataag ataggatatc cagtagttgt aagaccagca tatacgctag
     2761 gtggaactgg tggaggtata gctgatactg aggaagaatt aagagaaata ctttcacatg
     2821 gactacagct aagtcctgtt ggacaagttc ttcttgaaaa gagtataaaa ggttggaaag
     2881 aaatagaata tgaagtaatg agagatggaa atggaaactg tataactgta tgtaatatgg
     2941 aaaatgtaga cccagttgga gttcatacgg gagacagtat agttgttgct ccaagtcaaa
     3001 ctctaagtga tgaagagtat caactactta gaaaggcatc aatagacata ataaatgcaa
     3061 tagaggttca aggtggatgt aacgttcaaa tagctttaaa cccacatagt ttagaatatg
     3121 cgataataga aataaaccca agagttagta ggtcatcagc cctagcatca aaagctacag
     3181 gatacccaat agcaaaagta gcagctaaga tagctttagg ttatactttg gatgagatag
     3241 aaaatgcagt aactaaaaaa acttatgctt gttttgagcc aacattagat tatgtggtag
     3301 taaaaatacc aaagtggcca tttgataaat ttagacatgc caatagaaaa ttaggaacta
     3361 agatgatggc aactggtgaa atcatgagta taggaagtaa ctttgaggct gctattctta
     3421 aaggtataag atcacttgaa acaggaaaat attcattggt tcatgctcca tcagaaggta
     3481 gaacattaga agaattaaag gctagagtag tagtacctga tgatgagaga ctatttgact
     3541 tagctgaaat gataagaaga ggctataaag tagaaatgat atcagaaata actggtgttg
     3601 ataagtggtt tataaataaa tttaaatgga tagtagaaca agaagaaaag ttaaaagttc
     3661 taaaaataga agacttagat aaagaatatt tacatgaatt aaagaaaaag ggattttctg
     3721 ataaaggtat atctgactta atgaagataa gcccagaaaa attatatgaa ttgagaagtt
     3781 tatataatat acaaccagtt tataaaatgg ttgatacatg tggaggagaa tttgaagctc
     3841 tatcacctta ttactattca acttatgaac aatatgatga ggtagtagtt agtgataaga
     3901 gaaaagttgt agtattgggt tctggtccaa taagaatagg tcaaggaata gaatttgact
     3961 attgttcagt tcactgtgta aaatctttaa gaaaaatggg aatagaaact ataattgtaa
     4021 acaataaccc tgaaactgta agtacggatt ttgatacatc tgataagttg tattttgaac
     4081 cattaacaga agaagaagtt ttaaatataa tagaaaaaga aaaaccagaa ggtgtaatac
     4141 tacagtttgg tggacaaaca gctataaaat tggctaagtt tttacatgaa aaaaatatac
     4201 ctatactggg aacagacttt agagatatag atgcagcaga agatagagaa aaatttgatg
     4261 atttactaga gagactagat ataaatagac caaaaggaaa aggtgtatgg acaactaatg
     4321 aaggtgttga aattgctaag gagttaggtt atccagtatt agttagacca tcttatgtac
     4381 ttggtggtca aggtatggaa ataacttata acgaagaaaa actaattcag tatttggatg
     4441 atgcatttga tagagaccat aaaaatccag tacttataga taaatatcta actggtagag
     4501 aaatagaagt agatgctata tgtgataaag aagatatatt gatacctggt ataatgga
//
LOCUS       DAELJO010000257         4397 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELJO010000257 DAELJO010000000
VERSION     DAELJO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.259"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>4397
                     /gene="tcdA"
                     /locus_tag="KPB88_003577"
                     /gene_synonym="toxA"
     CDS             <1..>4397
                     /gene="tcdA"
                     /locus_tag="KPB88_003577"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0055812.1"
                     /translation="SLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENKY
                     LQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIG
                     GEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQ
                     NPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETV
                     LESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLALK
                     NFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFD
                     KLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGSY
                     LTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIAP
                     YLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLSQ
                     LTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKIP
                     SNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLSD
                     DGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLDT
                     IKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIGA
                     NQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPGL
                     ASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDDL
                     IDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVET
                     EKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLIDY
                     SSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEGI
                     PIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIGA
                     EVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKILV
                     PIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSIY
                     SAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPGK
                     FYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGAG
                     GTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDIN
                     KNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNYL
                     ISNLSNTIEKINTLGLDSKNIAYNYTDES"
BASE COUNT         1810 a          530 c          641 g         1416 t
ORIGIN      
        1 gtctttaata tctaaagaag agttaataaa actcgcatat agcattagac caagagaaaa
       61 tgagtataaa actatactaa ctaatttaga cgaatataat aagttaacta caaacaataa
      121 tgaaaataaa tatttacaat taaaaaaact aaatgaatca attgatgttt ttatgaataa
      181 atataaaact tcaagcagaa atagagcact ctctaatcta aaaaaagata tattaaaaga
      241 agtaattctt attaaaaatt ccaatacaag ccctgtagaa aaaaatttac attttgtatg
      301 gataggtgga gaagtcagtg atattgctct tgaatacata aaacaatggg ctgatattaa
      361 tgcagaatat aatattaaac tgtggtatga tagtgaagca ttcttagtaa atacactaaa
      421 aaaggctata gttgaatctt ctaccactga agcattacag ctactagagg aagagattca
      481 aaatcctcaa tttgataata tgaaatttta caaaaaaagg atggaattta tatatgatag
      541 acaaaaaagg tttataaatt attataaatc tcaaatcaat aaacctacag tacctacaat
      601 agatgatatt ataaagtctc atctagtatc tgaatataat agagatgaaa ctgtattaga
      661 atcatataga acaaattctt tgagaaaaat aaatagtaat catgggatag atatcagggc
      721 taatagtttg tttacagaac aagagttatt aaatatttat agtcaggagt tgttaaatcg
      781 tggaaattta gctgcagcat ctgacatagt aagattatta gccctaaaaa attttggcgg
      841 agtatattta gatgttgata tgcttccagg tattcactct gatttattta aaacaatatc
      901 tagacctagc tctattggac tagaccgttg ggaaatgata aaattagagg ctattatgaa
      961 gtataaaaaa tatataaata attatacatc agaaaacttt gataaacttg atcaacaatt
     1021 aaaagataat tttaaactca ttatagaaag taaaagtgaa aaatctgaga tattttctaa
     1081 attagaaaat ttaaatgtat ctgatcttga aattaaaata gctttcgctt taggcagtgt
     1141 tataaatcaa gccttgatat caaaacaagg ttcatatctt actaacctag taatagaaca
     1201 agtaaaaaat agatatcaat ttttaaacca acaccttaac ccagccatag agtctgataa
     1261 taacttcaca gatactacta aaatttttca tgattcatta tttaattcag ctaccgcaga
     1321 aaactctatg tttttaacaa aaatagcacc atacttacaa gtaggtttta tgccagaagc
     1381 tcgctccaca ataagtttaa gtggtccagg agcttatgcg tcagcttact atgatttcat
     1441 aaatttacaa gaaaatacta tagaaaaaac tttaaaagca tcagatttaa tagaatttaa
     1501 attcccagaa aataatctat ctcaattgac agaacaagaa ataaatagtc tatggagctt
     1561 tgatcaagca agtgcaaaat atcaatttga gaaatatgta agagattata ctggtggatc
     1621 tctttctgaa gacaatgggg tagactttaa taaaaatact gccctcgaca aaaactattt
     1681 attaaataat aaaattccat caaacaatgt agaagaagct ggaagtaaaa attatgttca
     1741 ttatatcata cagttacaag gagatgatat aagttatgaa gcaacatgca atttattttc
     1801 taaaaatcct aaaaatagta ttattataca acgaaatatg aatgaaagtg caaaaagcta
     1861 ctttttaagt gatgatggag aatctatttt agaattaaat aaatatagga tacctgaaag
     1921 attaaaaaat aaggaaaaag taaaagtaac ctttattgga catggtaaag atgaattcaa
     1981 cacaagcgaa tttgctagat taagtgtaga ttcactttcc aatgagataa gttcattttt
     2041 agataccata aaattagata tatcacctaa aaatgtagaa gtaaacttac ttggatgtaa
     2101 tatgtttagt tatgatttta atgttgaaga aacttatcct gggaagttgc tattaagtat
     2161 tatggacaaa attacttcca ctttacctga tgtaaataaa aattctatta ctataggagc
     2221 aaatcaatat gaagtaagaa ttaatagtga gggaagaaaa gaacttctgg ctcactcagg
     2281 taaatggata aataaagaag aagctattat gagcgattta tctagtaaag aatacatttt
     2341 ttttgattct atagataata agctaaaagc aaagtccaag aatattccag gattagcatc
     2401 aatatcagaa gatataaaaa cattattact tgatgcaagt gttagtcctg atacaaaatt
     2461 tattttaaat aatcttaagc ttaatattga atcttctatt ggtgattaca tttattatga
     2521 aaaattagag cctgttaaaa atataattca caattctata gatgatttaa tagatgagtt
     2581 caatctactt gaaaatgtat ctgatgaatt atatgaatta aaaaaattaa ataatctaga
     2641 tgagaagtat ttaatatctt ttgaagatat ctcaaaaaat aattcaactt actctgtaag
     2701 atttattaac aaaagtaatg gtgagtcagt ttatgtagaa acagaaaaag aaattttttc
     2761 aaaatatagc gaacatatta caaaagaaat aagtactata aagaatagta taattacaga
     2821 tgttaatggt aatttattgg ataatataca gttagatcat acttctcaag ttaatacatt
     2881 aaacgcagca ttctttattc aatcattaat agattatagt agcaataaag atgtactgaa
     2941 tgatttaagt acctcagtta aggttcaact ttatgctcaa ctatttagta caggtttaaa
     3001 cactatatat gactctatcc aattagtaaa tttaatatca aatgcagtaa atgatactat
     3061 aaatgtacta cctacaataa cagaggggat acctattgta tctactatat tagacggaat
     3121 aaacttaggt gcagcaatta aggaattact agacgaacat gacccattac taaaaaaaga
     3181 attagaagct aaggtgggtg ttttagcaat aaatatgtca ttatctatag ctgcaactgt
     3241 agcttcaatt gttggaatag gtgctgaagt tactattttc ttattaccta tagctggtat
     3301 atctgcagga ataccttcat tagttaataa tgaattaata ttgcatgata aggcaacttc
     3361 agtggtaaac tattttaatc atttgtctga atctaaaaaa tatggccctc ttaaaacaga
     3421 agatgataaa attttagttc ctattgatga tttagtaata tcagaaatag attttaataa
     3481 taattcgata aaactaggaa catgtaatat attagcaatg gaggggggat caggacacac
     3541 agtgactggt aatatagatc actttttctc atctccatct ataagttctc atattccttc
     3601 attatcaatt tattctgcaa taggtataga aacagaaaat ctagattttt caaaaaaaat
     3661 aatgatgtta cctaatgctc cttcaagagt gttttggtgg gaaactggag cagttccagg
     3721 tttaaggtca ttagaaaata atggaactaa attacttgat tcaataagag atttataccc
     3781 aggcaaattt tactggagat tctatgcctt tttcgattat gcaataacta cattaaaacc
     3841 agtgtatgaa gacactaata ttaaaattaa actagataaa gatactagaa acttcataat
     3901 gccaactata actactaacg aaattagaaa caaattatct tattcatttg atggagcagg
     3961 aggaacttac tctttattat tatcttcata tccaatatca acgaatataa atttatctaa
     4021 agatgattta tggatattta atattgataa tgaagtaaga gaaatatcta tagaaaatgg
     4081 tactattaaa aaaggaaagt taataaaaga tgttttaagt aaaattgata taaataaaaa
     4141 taaacttatt ataggcaatc aaacaataga tttttcaggc gatatagata ataaagacag
     4201 atatatattc ttgacttgtg agttagatga taaaattagt ttaataatag aaataaatct
     4261 tgttgcaaag tcttatagtt tgttattgtc tggagataaa aattatttga tatccaattt
     4321 atctaatact attgagaaaa tcaatacttt aggtctagat agtaaaaata tagcgtacaa
     4381 ttacactgat gaatcta
//
LOCUS       DAELJO010000258         4327 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELJO010000258 DAELJO010000000
VERSION     DAELJO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.260"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            116..1546
                     /locus_tag="KPB88_003578"
     CDS             116..1546
                     /locus_tag="KPB88_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0055813.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(1694..2569)
                     /locus_tag="KPB88_003579"
     CDS             complement(1694..2569)
                     /locus_tag="KPB88_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0055814.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLDEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESVNSAMDILKSGEADICFCEGIEPHGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDIENKMLAILHKDKNI
                     TQPFEIILDNISNVL"
     gene            2689..3252
                     /locus_tag="KPB88_003580"
     CDS             2689..3252
                     /locus_tag="KPB88_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0055815.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRLAGTLGVVISCTAAIIPPLVILGLVSVF
                     YTTFISNAIATAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSVMIPIAFFANFVM
                     SINVIFILSVCCCVCVMQVFYKKRENK"
     gene            3249..3815
                     /locus_tag="KPB88_003581"
     CDS             3249..3815
                     /locus_tag="KPB88_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0055816.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKLASLGLIISAAATIIILVLFGTNKVYFNSIFEILNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGTFFYL"
BASE COUNT         1592 a          526 c          716 g         1493 t
ORIGIN      
        1 tattagaagt atataaaagt attttatgtc tatgaaatgt atgtctataa aacatataga
       61 tgacttttgt atattaatgt atgaatattg atattataga agaattgagt tgtcaatgat
      121 actaaatcaa atatttatag aagctggagg gattatgaga aataaaataa ttataggaat
      181 tactttttca cttgtattac taatgtctgg atgtgcttca aaacaaaccg cagaaataaa
      241 cgtattagca actactgatt tacatggagt tataccttat gagttaactt cttatgtaaa
      301 agaagaaaga aaaaaagata aaaatctaac tttagtagat gcaggagatt tttttgatag
      361 tggagaagtg tttggttcta gcatggatag ttattttagt gctagatata aagatggtga
      421 taataacact gaaagttata ttgagacgcc tatttcaaaa gatatgaaag aggtaggtta
      481 tgatgttgca gttcttggga atcatgaatt tatatccaac aataaatttt atctagataa
      541 tatgatatct gattttgaga agcaaaaaat aaatatttta tcggcaaata cgtataaaaa
      601 aaatggagac agttatacaa aaccatacat aataaaaaat attaatacac ctgaaggaaa
      661 cgtaaaacta ggaattttag ggcttactat aaaagaagtt ggtgagagga aacagtggaa
      721 agatggaaaa cttgttgact ctaagtcttt agaactaaaa gaccaagagg gctacaatgg
      781 tgaattgtat atgaatgatt tagtagaaga tgctaaaaag tgggttagta taatggaaaa
      841 agagaagcct gacattatag ttgctgtggc acatacaggt gagaaaccta agaaacctag
      901 aaacccagga aatagaatac aagacttagc tcaacaagtt gatggaattg atgctatagt
      961 tgcaggtcat aatcatgttc aaattgagca acatgattat aaaaataaat caggagaaaa
     1021 tgttatagtt actgagccag gaaagcatgg agaatgtatt tctaagatta attttaaatt
     1081 agaaaaaaac aatgataaat ggaatatagt tgacaaatct agtaaattaa ttaaatttga
     1141 aaaaaatcaa gaaagtgata aatttggaga atttatttct tcaattaata aagtaagtaa
     1201 agataagaaa gaaataagat tatcagaagt aaagactcct gaatgggata aaatatatgt
     1261 ttttaaatct ggaacatctg cagatgatat atataaaacg ataggatata aatggagaaa
     1321 tatatcatct ccaaagggtg atgacatggt gcagattgtt gtaatgaata ataataagcc
     1381 tgttagctat gtatatggtg aagctgaagt tatgccaatt aatataaaat gtgatgaatc
     1441 agagtataaa gataatgttg taactattta tcctaataaa aatgataaat ttaaaattga
     1501 aaaaggtaaa caagaatata gtattaattt aaaacatata caatagaaac ttttatctta
     1561 tttaagttta gattgtactt aaaataaagt gtgtattatt ttaaaaagtg atgataatta
     1621 aaatcacaac attaaggaat agtacacact ttttatgcta aaaaatagaa tattaaaaaa
     1681 ttaaaccttt aaattaaagt acattgctta tattatccaa tattatttca aaaggttgtg
     1741 taatattttt atccttatgt aatattgcta acattttatt ttctatatct atcccatcta
     1801 attcaatcaa tctcaatttt ttatgtaaga gtgaatctaa taataaattt tctggcaaaa
     1861 tggttatacc taaccctgct tctgcagctt taataagtgc aaaagaatta acactttccc
     1921 aaacaggata agctttttgg tttgaaagag ataatacact gtccaaagta tcgcgaatag
     1981 cacttccttg ctcacgtaac aataacggaa acgtgcataa ctgtcgtaga gataaggttt
     2041 gttcagataa aggaaaagtt ggagcacatg caacacataa cttataggaa cctaataaaa
     2101 ttgtacggaa tgccccatgt ggttctatac cttcacaaaa acatatatca gcttctccac
     2161 tttttaaaat atccatagct gaattaacac tttcgacacg tacattcact ttaagagatg
     2221 gaaatgaatt ttttagtcga cttaaaattg gtggaagtaa aaaagaggca attgtaatgc
     2281 tagaaacaat gtttataggt gtacattcct ctaaacgact gattcttttg tctaaattgc
     2341 gacaagcagt taagatacta cgtgcttcat ctaaaagaga aatgccacag cgagtaagac
     2401 atacccctcg tggtaatctg tcaaatagtg ctgtttcagc ttgttgttca agttcagcta
     2461 tagcatgaga tacagcagac tgtgtgatat gtaatttttt tgcagcacct gtaaaagtgc
     2521 ctgtttgttc aattgcttct aaaatctcta aatatcgtat gtttatcata ataaatatcc
     2581 tcactagaca tgaataaaat taattaattc cattaaattc tatcatttta cagattgcaa
     2641 agcaacaggt atactgaata atatgaagtg aaaagaagga ggaatatttt gaatcaaagc
     2701 agaaaaacat atttttggct tttaattatt aacttgttta ttagcacatt cacttttggt
     2761 ggagggtatg tagttgtacc tatgattaga cgctattttg taaatcaaaa gcaattcttt
     2821 acagaagatg atttgatgag tatggcagca gttgcacaat caactcctgg agctattgca
     2881 attaatttat ctgctttggc aggttatcga ttagctggta ctttaggggt tgtcattagt
     2941 tgtacagcag ctataatacc tccactagtt attcttggat tagtatcagt attttacaca
     3001 acttttatat ctaatgctat tgcaactgca gtattaaaag gaatgcaagc tggtgttgct
     3061 gcactaattg tggatttgat tattgacatg tgctctatga tattaaaaca acgttctcta
     3121 tttttatcag ttatgatacc tattgctttc tttgcaaact ttgtgatgag tataaatgta
     3181 atttttatat tatctgtttg ctgttgtgta tgtgtaatgc aagttttcta caagaagagg
     3241 gaaaataaat gaatacaatt tggaacttat tttttatatt tttaaaaata ggcttgttta
     3301 gtattggtgg tggttatgca atcatacctt taatccaaga gaaagtagta aatcaaactg
     3361 gatggataag tgagaaaatg tttacagata ttataactat ttcacaaatg acaccagggc
     3421 cattggctgt taatacttct acttttgttg gacttcaaat tggaggtttt tggggagcag
     3481 ttgcagccac tttaggttgt gtattatgtg gtgtagtaat atctctattt ttatataatt
     3541 tttttcaaaa gcatcagaac tctacttata tatttgaaat attaaatggt ctaaaattag
     3601 cttcattagg tttaattatc tcagctgcgg ctacaattat aatccttgta ttatttggga
     3661 cgaataaagt gtacttcaat tctatatttg agattctaaa ctggactgca ttaataatct
     3721 tttttgttac gctactgata gtacgtaagt ggaaaattaa tcctattgtg attataataa
     3781 taagtggtat actgggaaca tttttttatt tataagtttt tggtaagtaa aggatatcta
     3841 tttttttata tcttaaatcc aaatagccaa taaagtaagg ctatttggat ttttaataaa
     3901 ttctgaaaag ataatcagaa acccatataa catttagatt atatcactgg ttttctacaa
     3961 accaatcata agcttcttgg caaaatttct cactatctat atagtaacta ttatgaacat
     4021 tattaatttc aacaagcttc tttttacatt ttagtgaatt ataatagcta tcaaaatatt
     4081 gggctttaaa atatctatca tgaagtgttt taaagataag agtaggtatt tgactccatc
     4141 ttttatctta ctatcttctg caataaagca ttctaacatt gtattaatta tctttgtact
     4201 atttttccaa atatgaggat ttttcttttc aaatatttta taaaattcat tatcatagac
     4261 ttcattataa cctttatatt taggtggttt tactgttatg tgaaaattag gaagaaactt
     4321 aaagaaa
//
LOCUS       DAELJO010000259         4305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELJO010000259 DAELJO010000000
VERSION     DAELJO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.261"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            412..891
                     /locus_tag="KPB88_003582"
     CDS             412..891
                     /locus_tag="KPB88_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0055817.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            888..1667
                     /locus_tag="KPB88_003583"
     CDS             888..1667
                     /locus_tag="KPB88_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055818.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1917..2417)
                     /locus_tag="KPB88_003584"
     CDS             complement(1917..2417)
                     /locus_tag="KPB88_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055819.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHAYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3029..3736)
                     /locus_tag="KPB88_003585"
     CDS             complement(3029..3736)
                     /locus_tag="KPB88_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0055820.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1577 a          638 c          465 g         1625 t
ORIGIN      
        1 cattgttttt tcttaaaagc ctacttttag tcaagatact gttatcttta ctcattattt
       61 aaagaactta ttttaaatct gtacatgaag taaataatcc aaaagcatta ttattctaac
      121 tactcaacat taaatactta ctattttcta attactcaat ataaacactt actataaaac
      181 taaatatata aatggcttct aaagtttaat atatctacat ggctttatat atgacaggaa
      241 aaatactaga aaaaatatat tatagaatac ttgtatataa taggcaaaac tttcaaactt
      301 aaatttatca aataatcata aaaaaagtga accatctata gtaaaatttt atctaataag
      361 tgaaaggcaa aaaagtagcg ctacgatttg catagaaggg aaggaactta gttggataaa
      421 actacattta caaacaatat acttgaatca gaacagactt tgtatcgagt atctaagtct
      481 attctaggaa acgaccaaga ctgtgaagat gccgtcaaca atgctatttt aaaagcatat
      541 gaaaagttgg attctcttaa ggaagaacaa tactttaaaa catggctgat tagaatagtc
      601 ataaatgaat gtaattcatt aagacgcaaa cgattaaaat ctctatcatt tgaagatgtc
      661 ttcaaaaaca aaaaaattga tgaaaaagat gactatagcg atttgtatac tgctattcaa
      721 agtctaccta aaaaaattaa aattccaata gtcctttact atattgaagg ttattcagta
      781 gatgaagtta aggaaatatt agatatacct caaggaactg taaaaagtag attatcaaga
      841 ggaagaagat tattaaaaac taaattagaa aacacggagg taatcatatg aataaaaata
      901 tattaaataa ttcgtttcca gaagttccag aaagttttca taatagttta agcaaaacac
      961 tgaatagttt accagaaaga gaggaaaatt ataaaatgat taataataaa acacacaaat
     1021 taccatttag aaaaggttta gttgcaactt tagccattac tttgatattg agcacaacag
     1081 cccttgcagt aggacatata tatagtttag ttggaaaatc aagtactaag gctacctata
     1141 catctatccc aactacagaa caagtaaaaa aagactttaa ctttactcct aatatagttt
     1201 ctgaatttag taatggatat aaatttaaag gagcatatac tgtaaataaa aaagcatttg
     1261 acaaagaaaa taatgtttta ggaaaatcaa aagcacttag ttttgaatat aaaaatggca
     1321 atgatgaaat atctcttgac acagcaaata atgtacttgg gaagagttct gacaatctaa
     1381 aagttgtaga tacttacaaa gatattaata tttcttatga agcattcgca caaaagtttg
     1441 taccagaagg ttataaaatg acagaacagg ataaaattga tgaaaaatca ggcaaataca
     1501 catttagtgt agggccaaat aatgaatcag acaaagttga agttaataaa tttaagtttt
     1561 tgacatggaa acaagatgga gtttactatt cttttacagt tcaagactca aatttatcta
     1621 tgaatgactt agttaaaatg gctcatgaag taatagatgc aaaataaaca taagtaataa
     1681 taagttgttt atgagtttat tatttatgac tttaattttt ttattagtaa acatggttgt
     1741 atcttccatc tttatatttt ctttaaattt aatatataaa attttagata tttagctacc
     1801 ttcaaaagaa ccattattaa aaagccctta aaaatatttc ttttatatgg aattttaagg
     1861 gctttaactt tattttatac aagtgtatat ggaattttaa agactttaac tttattttat
     1921 ataagtgtaa gctccatttg aattacttct tcttttacag gtctatcatt ccatgtaaaa
     1981 ccactagata ttgtatttac ctctacaaac cctaaagatt tataagcatg aatagctcta
     2041 ctattacttt taaatacatc aagctcaatt ttttcatacc catattcttt tgataaatca
     2101 ataatttgtt ttaaaagact acttccgata cctttttccc tatattcttt aagaataccc
     2161 atgcctaaat aaccaacttt ttcagtttta ggtaaaacgt cacaccaacc aatacagtta
     2221 tctgattcta aatcaattat aaacagttgt ggtaagtttt tatttataat attttttata
     2281 aattcaactg tttcttcaaa aggaaaggct tcatccatag ctagatattt cctctctttt
     2341 gctattttat caaaagtttg ccaatacgaa taaatatact tttcttctgc ctttataaat
     2401 tttatgcctt ctatcaactt aacatttccc ctttttaatt tgtgattgta gttaacttat
     2461 tttatatgat tctagtttat aatgctatat aaattactat atattagctt aaaatatctt
     2521 cgtatatttc tataaaatta aaaccaatat atctatctct tgtatctcta ttaaaataat
     2581 ttttaatagt ataataaata taactcttta atcttttgag tatatatact taatcatttt
     2641 gaaatttgat ttaaagtata ataaaatata taaaaaaata tataaaatgg tactataaaa
     2701 tttttagtct tcatcaatac cttttttgct ttcattgtta aaactatttt ttctattagt
     2761 ttatttctta taaaacaaac atttagtatt aaaatatata tcacacaata gtatttgcaa
     2821 tttaacttta aaaaaataaa aatacccaac tgacctaagc aactcgattg ggtataaata
     2881 atctaaaaaa tcatttttgt ataaactctt tatttaactt acatttttat ttaccttcta
     2941 ctttaattca acttatattt ctatgcttct tatactctca ttcaacttat atttctactt
     3001 atcttatact tttataaatt catcacaatt atttagttct cgctatcaaa gaaattgtat
     3061 taaaatactt tccattctct acttccatca tttttttgtc atttatagca tatgaattat
     3121 cacaatttat ccaatccttc caagcttcat catgacaatt taaagcttca catttctcta
     3181 caatcacaga ctccgattca gaccaaagtt tattccacca tgtaattgaa tggaagttca
     3241 tattatcttg ccaaaatgga atcagttcat ctggtataca atccacgaaa tcttctttta
     3301 atccaggcat tgccatagca agtattcccc cctcttttac aagaggtgat atatgattgt
     3361 ctaaaaatcc ttctttgttt ccaaaatagt gataagaatc gatacttatc accgcatcaa
     3421 agaaaccttc tgcataaggt agttcatgtg cctctgcctg tattggaaat attttatcat
     3481 ccaatttaaa ctctttaaat cttttatagt tttcagttgg ttctatccat aaatctgttg
     3541 caaatacagt agcatcaaat tcctttgcta aaaatatcga agatatacct ttaccacaac
     3601 ctaagtctaa aatacgcatt cccttttcta gttttatctt acttgttaat tcttccaata
     3661 tttttataca attaggtccc atcatatttt tcatgagaaa gtccttatcg tatttattac
     3721 tttttgtaaa tatcattcta tctttccttt ccctatcagt tattatttat taaattttat
     3781 acgtttgatt ttttattttt tatatgcttc ttatattgag caatctcttt tttattcatt
     3841 ctttaacaga caattttaat gtgtaatttt atccactctg tgatagtaac tttaatgaat
     3901 aatcttatct actgtgatag ttaactttga taggtaattt tatctactct atgataatta
     3961 ttttagtgag taattttatc tactccacag caatcgcttt aatgagtaat cttatccact
     4021 ctatgataat tattttagtg agtaatttta tctactctac agtaatcgct ttaatgagta
     4081 atcttatcca ctctatgata gttattttag tgagtaattt tatctactcc acagtaatcg
     4141 ctttaatgag taatcttatc cactctataa tagtcacttt aatgagtaat cataattttt
     4201 atatcatttt agatacttac tctaataaga attcattttc taatacttaa taagttttaa
     4261 ttacttccac tttaacctat atactaatta acttatttaa tttag
//
LOCUS       DAELJO010000260         4252 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELJO010000260 DAELJO010000000
VERSION     DAELJO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.262"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            610..1518
                     /gene="trxB"
                     /locus_tag="KPB88_003586"
     CDS             610..1518
                     /gene="trxB"
                     /locus_tag="KPB88_003586"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0055821.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1608..3431)
                     /gene="typA"
                     /locus_tag="KPB88_003587"
     CDS             complement(1608..3431)
                     /gene="typA"
                     /locus_tag="KPB88_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG0055822.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3643..3885
                     /locus_tag="KPB88_003588"
     CDS             3643..3885
                     /locus_tag="KPB88_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055823.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4033..4224
                     /locus_tag="KPB88_003589"
     CDS             4033..4224
                     /locus_tag="KPB88_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG0055824.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1569 a          548 c          674 g         1461 t
ORIGIN      
        1 aagtaatcaa aagataacta ttaaatatct gtaatattac aattttatac tacactatat
       61 tacaaaaaaa gtttacaaaa ctttaatttg aaaaatgtaa ttatatggta tatcatataa
      121 atataagtaa atagtgctaa tatttaaact attaatcata attggttcaa caaaagtata
      181 tagttaataa tgtttaattt gaagaaattg ttagtgtgaa attacatata tattcaaatt
      241 aaattcataa atatttcaca taataatagt atactgtaaa caaaaggagg tattaaatat
      301 gggaatagta cttataacag cagcattagt tttggttatg tatggacttt caattctatc
      361 atatatatca gaaagcaata tattagaaga cactgtaaat gataaccaat aaggttttga
      421 attatataaa taagtgagaa ctgtaaataa aagttaattt ttatttgcag tttttttata
      481 tatatgtaaa ataaagttaa ttaataaaaa ataatgtttt gtttatagta tatatggata
      541 ataatagtaa agtataagaa tgatgagaat aatatataag aaataataat ataaagagag
      601 gtaaacgtta tggtagatat cattgtaatt ggtgctggac cagcaggttt aacttcagca
      661 atttatgcta tgagagcagg gttaagcgtt acagtatttg aaaaaaatat ttatggaggt
      721 caagttgcaa gtacctctga ggtagaaaat tatccagcag ttcaaaagat ttctggggta
      781 gaattttcta ataatatata caatcaagca gttgctcaag gtgttgatat tcagtttgat
      841 gaggttgaag agataaattt agagggaaaa gtaaaggttg taaaaacttc ttcaggtgaa
      901 cataaagcaa aggctgttat tcttgcaaat ggagttgaaa gacgtaaatt aggttgtgca
      961 ggggaacaag aatttacagg tagaggagta tcttactgtg caacatgtga tggagccttt
     1021 tttaaagata aagaagttgc tatagtaggt ggaggaaata ctgcactaga agatgcttta
     1081 tttttagcta ataattgtac aaaagtgtat ttaattcaca gaagagatag ttttagaggt
     1141 gaagaagtgt tggagaaatc tgtgaaagca agagagaata ttgagatact ttacagtcat
     1201 ggtgtagaaa aaatagaagg agaaaagact gtatcgaaaa tagaagtaaa aaacttaaag
     1261 actgaagaaa aaagaactat agatgttagt ggtattttta ttgctatagg tcttaaacct
     1321 aataataaaa tgtttgaaaa tgttcttgat ttggatgaag gtggatatat aatttcagat
     1381 gagtcttgta caacatcagt agaaggtgta tatgtagcag gagatagtag aactaagttt
     1441 ttacgtcaaa taataacagc agcatcagat ggagctattg cagccgttca agcagctaat
     1501 tatataaatg ttgaataata taaatttcaa ataaaaatag ccatataaac tgaaataatt
     1561 tttcagtact atatggctat tttgttaatt agaggtaaat gtacgtttta tttaccttgt
     1621 ctagatttgt tatatcttaa tctatcatgt tcatttaaga atctttttct aagtcttata
     1681 gaatcaggtg taatttcaac taattcatca tcttctataa actctagtgc ttcttcaaga
     1741 gtaaatattc taggaggaga taattttata gcatcatctg aaccagaagc acgtacgtta
     1801 ctcattttct tgtttttaca agggttgact accatatcat cttttctgga attcatacca
     1861 attatcatac cttcgtaaac ttctacacca gggtctacaa acattacagc tctatcactt
     1921 aaggcattaa gagaataccc catagtaaca ccaggtcctt gtgctattaa aacaccattt
     1981 cctcttgaag gtatttctcc tttaaattct tcaaattttt caaatgaacg aactaaagta
     2041 ccttctccac gagtagcgtt tataaattca cttcgatatc ctaaaagacc acgagtaggt
     2101 gctaagaact ctatttttac atagtctcct tctattgaca tagactccat catacctttt
     2161 ctcatgttta attcatttat aatagtacca gagtaaactt caggacagtt tacaacaact
     2221 ctttctattg gttccattaa ttttccatct tctttatgca ttaaaacttc aggtttagaa
     2281 acaccaactt cataaccttc acgtctcata ttttcaagta gtatagataa gtgaagctct
     2341 ccacgtccag aaactttgta tccatcagta gtatcaagag gttcaacttt aagaccaaca
     2401 tttacttcaa gttccttttc aagtctatct ttcaaatgtc tagttgtaac gaattttcca
     2461 ctttttccaa cgaatggaga gtcgtttact aagaagttca tagaaagagt aggctcttct
     2521 atttttatca tttccatagg tagtggacta tctaaatcgc atatagtttc acctatagat
     2581 atatcaggaa taccagctat aacaactatt tctccactag tagcttcatc aacttcaact
     2641 tgttttaaac cttcatatac agaaagtttt gatacttttc ctttagatac aacagaatct
     2701 tctctacata tagcaacttg tgtattgttt ttaagagtac ctttgtaaat tctacctata
     2761 ccaagtctac ctacataatc atcatatgca agagctgata tttggaattg tagaggttca
     2821 ttatcgtaat taggataagc ttcaacgtga tttacaatag ttttaaatag aggagaaagg
     2881 tcttcactat catcatccat ttcaagttta gcaatacctt gtttagcaat accatatatt
     2941 ataggaaatt cgcattgctc atctgttgca tttaagtcta caaataaatc gaaaacttca
     3001 ttaactactt cttccgctct ctggtctttt ttatcaatct tgtttataaa taatattggt
     3061 tttaaaccga actctaaaga tttttgtaaa acgaatctag tttgaggcat tgggccttca
     3121 ctagcatcaa ctaatagaat aactgtgtca acagttttca taacacgttc aacttcagaa
     3181 gaaaagtcac tgtgtcctgg agtatcaact atgtttattt tgtaatcttc atagtttatt
     3241 gcacagtttt ttgaatatat agttatgcct ctttcttttt caaggtcgtt actgtccata
     3301 acgcagtctt taactacttc gttctttcta aaaacaccgc tttgagaaag gaaagcatca
     3361 accaatgttg attttccagc gtcaacgtgg gctataactg cgatatttat aattttatgt
     3421 ttttgtgaca tttttgttgc tcccttcgat taaaataact tattaattat agcacaatat
     3481 attattaggt acaaggagga atatattaaa ttgtattaat atagtttaaa ttggataaaa
     3541 ctgttagttt tgcatctgaa aattattctg tttaaaaaaa ttttttagac aaataataac
     3601 cttaatagat aaaaagatta ataaaagagt aaaggatgat atatgttgga agtaaccaaa
     3661 ataataatta aatattttgc aagtgaaagt gatacttttg taaaagaaga agtttgtaat
     3721 tttgaagaac ttcttaagtg gtctcataaa tttattgaag gtaaaaagat agaagatgtt
     3781 ttgtatataa agtctaaaga aaactataaa gtaaatgtga ttaagtcttt agaaattaag
     3841 gaattggaaa tagtgagaaa acaacagata ccaccataca tgtagtatta ataatatata
     3901 aaattaatat gtaatactcg caataggata taaatggtat atatggtaat aatttatatt
     3961 aaaaaaactt ttataaagtt tatatttggg tataaaagta ttaattagac attaattagg
     4021 aggaacttaa atatgataag agtatgtcca aactgttcaa atgtggatgt aaataagcta
     4081 aaaactttgg ttggagaaga aaatgtaaag actggttgta taggtcagtg tagagcattt
     4141 aaaaaagaag ctgttggaat aatagacaat gagcttgaaa taaaggaaac agaagcagag
     4201 ttttttgaag catgtaaaga ataaatttta aataaaatat aaattataat aa
//
LOCUS       DAELJO010000261         4208 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELJO010000261 DAELJO010000000
VERSION     DAELJO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.263"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            205..570
                     /locus_tag="KPB88_003590"
     CDS             205..570
                     /locus_tag="KPB88_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055825.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            648..1202
                     /locus_tag="KPB88_003591"
     CDS             648..1202
                     /locus_tag="KPB88_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0055826.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            1422..2096
                     /locus_tag="KPB88_003592"
     CDS             1422..2096
                     /locus_tag="KPB88_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0055827.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2451..2547
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2601..2777
                     /locus_tag="KPB88_003593"
     CDS             2601..2777
                     /locus_tag="KPB88_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055828.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2964..3203)
                     /locus_tag="KPB88_003594"
     CDS             complement(2964..3203)
                     /locus_tag="KPB88_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055829.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(3348..4157)
                     /locus_tag="KPB88_003595"
     CDS             complement(3348..4157)
                     /locus_tag="KPB88_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0055830.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1541 a          581 c          501 g         1585 t
ORIGIN      
        1 agttatttta aagttaaatt tctatagtca tgttatatat ttatttactt atatcttact
       61 aattatttat gtactaaata tgaactacaa tagaagaaat ttagaactta ataaaacata
      121 aaatttatat acagttcata tatatttgtc atatcacaag acaagtgata taatttatta
      181 tcattttact gggggtgata tgctatgaaa ataagagctg aatatacatg tcctcttgaa
      241 attgtacatg acataatcaa aggaaaatgg aaaactatcc ttatatttga attaagacaa
      301 tctaataaaa cattttctga attgcaacat aatatttatg gaataaatca aaaaatgttg
      361 attcaacaat taaaagagtt acgtgatttt gggattattg ataaaataag ttctgctgga
      421 tatccattac atgttgaata ttttcttact aatcgtggta aaaaaatgtt aaaagcagtg
      481 gaaataatgc aagaaatagg aatcgaatat atgcttgaac atggtcaaca agaaattcta
      541 gataataaag gaatttgcta caataagtaa tcctatacat accaaaaagt aagtaattta
      601 cattttttac aatagtatta taataaaaat aaataggagg tataatcatg tctatagctt
      661 taatattgat tgatattcaa aatgattatt ttaaaaatgg aaaatgtgaa ttatttcaat
      721 cagaagaaac tgctgaaaat gctaaaaaaa tactactgtt ttttagaaaa aacaatttac
      781 cagttattca tatacagcat atatcaacag ataaaacagc ttcttttttt cttcctaata
      841 ctcatggttc agaaataaat aaaattgttt ttccactaga taatgaagaa attataatta
      901 aacacacacc agatagtttc tttgaaacag atttgcaaag tatccttgag aaaaaaaata
      961 ttactaatct agtaatctgt ggaatgatga gccatatgtg tattgataca agtgtaagaa
     1021 cagcaaagaa acttcgatac gacataactc taataagcga tgcatgtaca acgaagaatt
     1081 tatcttggaa taatatagaa attaatgcaa atactgtaca tcaagtattc atggcatcac
     1141 tacagaacag ttttgcagat gtaaaaaaca cagataaatt tttatctaac tatcacaaat
     1201 aaaaacatac actatgttaa aagagcaagt ttgatttatt taagcttgct ctttaatcat
     1261 ttaaaattag atgctttcta tcatttttat atacataata tacgtttata cattttatat
     1321 tcatatataa ctattatata aattttaaac tttactatat tcacataaaa aatgtagtat
     1381 tatagtatat ataatattat atagaagggg gtgctagata aatgaaccat catatattga
     1441 ttcaatttga atatatagca agaatcatta ctgctggttt ctgtggggct ttaattggat
     1501 atgagagaaa gaatagacta aaagaagctg gaatacgcac tcattttata gtggcattag
     1561 cttcagcact tataatcatt gtttccaagt atggatttaa tgatatagtg ggtactccag
     1621 gtattggtct agacccagcc cgcgttgcag cacaagttgt cagtggtata ggttttttag
     1681 gtgctggatt aatatttatt agaaatcaat cagtaagtgg tcttactaca gctgctggta
     1741 tatgggcaac agccggtaca ggtcttgcca ttggctctgg attatactta gttggtattg
     1801 tttcaagtat cattattgtt attgtacaga taacacttca tagagatagg aaatggatga
     1861 aactccctac agcagagcaa gtttctctta aaatttctaa cagcactgat tccatagaat
     1921 atattgaagg taaatttaaa aaatctaata ttgaaattat aaacatgaat attaaacgcc
     1981 ttgatgggga ttggttagaa gtagttatat atactaagtt acctaaagga tataaaaaaa
     2041 caaatttatt aaaattattt agcgataatc ctcttataga aactttagaa atataatata
     2101 aaaattgaag cttaaaaata ataatatatt taatcaaggt caatatataa ataattacgg
     2161 gatgagtcta tataaaattc atcccttgtt ttttgccatt catatatatt ttttcaagat
     2221 atttttgttc atctattatc tagccaagta acccttgtga actaaacttt ttaatataaa
     2281 aacttcaata caaaactaaa tataattttt attaaacatt ctatacatat agtttgtttc
     2341 ttgagtgaaa aatagatttc atattgttaa gatattgtta aatatttcaa ttttctgaca
     2401 agtatgttga acaataactt atcctagtgt attattttag ttgtagaata aaactaaatc
     2461 ggcaaaacta gagaaattta gtgacgcaaa gctataggga ctaacgctta taaaataaac
     2521 tatgagttat gtcagccagt tgccaaaaag atattgttct ttttgtttta tgaaagtttt
     2581 ttagggggat agtaatatat atgtttagag atgaaatgga taaatgtaca cacatgttaa
     2641 ctgcttatat tagtagttta tatgattatt gtgattttat agatacacag ctagatgatt
     2701 ttatactaga gtacggagaa aatgtagtag aatcttgttt acatcaagtg atggtattgg
     2761 taagtaagta taattaaata gaaattgttt tctagagaac cctttttagt tttatctaca
     2821 tttaaaaatg ttattatatt aaaaaactaa tctcccctat atgcaaagtt tcttccatac
     2881 aacaatttct tcccatttat actttttcac ttgtatcttt aataaaattc aacttcaaat
     2941 attaatatac ttcaaaaatt attctatttc ttatctttac accaattcct atcagtatta
     3001 tcctttatat aggttctaac tatttctcca caatctaaac ataaatctga atatatagta
     3061 gattgtactg gaaaagtttt atttataact ttatctaatt ttccatctcc tctatatttt
     3121 aatccaatag tttcagcata tatgccacca gttgttaaaa ttcccttttc aatatttaca
     3181 cttccacaat tagaacattt cacatttata accccctaaa caattatttt ttcatgctat
     3241 cttataattt aaaatataat ttgctaccga ttttataaca cagtgacaaa tcggtggcaa
     3301 atcaacattt tttcattcta tttaccatct aatccattga aatatagcta tttagttata
     3361 ggaacttgaa tttccgttac aaactcctct acaattccag tctcatgtat atctatcttg
     3421 tagatttcta tagggtcagc aattatctta taattgtttt cttctataaa tttaaacacc
     3481 ttctccatat acatcttatt attagaatta ctcccagtat aatttaaagt tacataatat
     3541 ccttcatcaa aaactatatt atatactttc tcattacttt ccagaaaaca aaatacagat
     3601 ttaaactcat taaatatacc tttattaatt gcttcagtag aaaaggcaga accaatattg
     3661 ttatttccaa gtatattaaa tctatcttca tattctttct gaagtttttg aattaaaaaa
     3721 tcgaagtctt catccctctt tatatcagca tttaattcta aagcttttct tttttctata
     3781 tatacaacct ctatctcatc tatttttgta ttttgaatta cactttcaat cgaactcaat
     3841 ctttttataa tattttcctt atggcttata aattcagcta tttttttatc aattaaatcg
     3901 agttcttcat ttaaaatatt tttcgtagac tctatacttc tgtcatctag gtattctttt
     3961 attttcttca ttggaaagtt caagcttcta aattctttta ttaaatttaa tttccatata
     4021 tctgatatat tatacattct atatccattt atatctctaa ctggtcttag aatgcctatt
     4081 tcttcataat acattaatga atctctacct attccatata ttttagatat ctctcctatt
     4141 ttatagtaat ctttcattta tataataaac tcctccgaaa tcttattttt attttaaatc
     4201 aacattat
//
LOCUS       DAELJO010000262         4207 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELJO010000262 DAELJO010000000
VERSION     DAELJO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.264"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            109..1707
                     /locus_tag="KPB88_003596"
     CDS             109..1707
                     /locus_tag="KPB88_003596"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG0055831.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1942..3951)
                     /gene="bclA1"
                     /locus_tag="KPB88_003597"
     CDS             complement(1942..3951)
                     /gene="bclA1"
                     /locus_tag="KPB88_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG0055832.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGATGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGPTGNTGVTGEIGPTGVTGPTGVTGEIGPTGATGPTGVTGEIGPTGVTGPTGVTGE
                     IGPTGATGPTGVTGEIGPTGATGPTGNTGATGSIGPTGVTGPTGATGSIGPTGATGAT
                     GVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGT
                     FTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYF
                     AGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1243 a          763 c          642 g         1559 t
ORIGIN      
        1 aatatttttt cactaaattt ttttcttttt acaacaaata ctcactacct tttgatataa
       61 tatttttatc aattattatt atattcaagt tttgaaagga gtattaaaat gagtttatta
      121 gttttcggac ataaaaatcc agatacagat tcaatatgtt ctgcaatttc attagcacat
      181 ttaaagaaca aactaggaat aaaagcaaca gcttatgctt taggagatat cagaaaagaa
      241 gctaaatatg ccttggatta ctttaaggta gatgcacctg aaattttaga taatgttaga
      301 atacaagtta gagatttaaa ttatgataaa gtagtacctt taacaccaac ttcatctata
      361 ttagaggctt ataaccttat ggatgaaaaa aatgttaaaa cactacctgt aacttatgat
      421 gatggaactt ttgctggtat cattactatg aaagaaatag ctaaaaatct acttcatcaa
      481 cactttacaa ctatacacac atcactatca aacatttgta aaaaccttaa tggtactatt
      541 cttgtggact gtggtgaaga tataaaaggt agagtagtca ctttatcatt tggaatggag
      601 actctaatag agactttaaa agaaggagac atagctattg ttggagatag atatgatagt
      661 attgaatatg ctattgatac aaaagttaaa cttttaatac ttactaatca tacagagatt
      721 tcaaaagatt tgctagctct tgctaagata aatggagtgt ctgttgtttc tgttgaaagt
      781 gacaattata aaacttctaa tgtaataaat caatgtagtt tcttagactc tatagaagct
      841 acagaaaatt taattacatt caaagaagat gattatatgg atgaaataaa agagattatg
      901 ctagaaacaa acttcagatc ttatccaata ttagatgatg ataataagtt tttaggttta
      961 gtttcaaaag gtcatctatt aaacccatct aaaaagaatg ttgttttagt tgaccataac
     1021 gaatatgctc aaagtgctga tggaattgaa caagcaaaca tagttgaaat aattgaccat
     1081 cataaacttg gtggtatttc tactgatgtt cctatgtcat ttagagttat gcctgttgga
     1141 tgcaatagta ctataattta tcaaatgtac aaagaaaata atgttgagat accttatgaa
     1201 atagccgggc ttcttttatc tgctatatta tcagatacat tattatttaa gtctccaaca
     1261 acaactgata tagataagaa agcttgtgaa gaattaagta aaatagcaaa agtagacatg
     1321 gaaaaatacg ctatggatat gtttaaatgc ggtacttctt tagatgaata tactatagaa
     1381 gaaatagtaa atatggactt taaagaattt aatatgagtg gacatagagt tggtattgga
     1441 caagtattta ccttagacat agactctata tttgctaaaa aagatgagtt tttatcttat
     1501 ataaattcga ctgattatga taaattaatt ctttcagtta cagatataat caaagaaggt
     1561 tcttatttaa tctataaagc agaagatagc ttaattgcta acgcatttgg tgttgaaggt
     1621 gttcaaggca catttgcacc aggtgttgta tcaagaaaaa aacaattagt ccctaattta
     1681 actactgcaa tcaaaaattt taaataatat aaaattttta aataacgaaa actgtctcaa
     1741 atctatattt gagacagttt tttattatat atatattgat aataaagtta aataaaaatc
     1801 cataaattta aaatttttat gaatatattt tattattatt gttattttat tcattctaaa
     1861 ataggtaaaa ctcaatatat aaaaatactt ttgtcattta aataaagcct aaatagtttt
     1921 taagattatt ttagacacgt tttaagtcat aacagtatca gctattctaa atatcgtcaa
     1981 agtagccgca gttcctgctg attgcccatt tcgtatccct actgttgtag atgaaaaatt
     2041 atttattgta aaagtagtcc caccaaataa aaaaccagca aaatatccag aaagtactcc
     2101 tccgcctcct ggctcagtag taagccctgc caaatctttt cctcctaaaa ttgggaatag
     2161 gatagcgaaa gaatattgat ttatacttga tgaacctggt tgtaatgcta ctgaaactga
     2221 tatataatac acaccatttt cttgtactgt aaacgtacca tttgcatcta ctgttatact
     2281 tgaactattt cctattattg tttgccaacc tgttatagca tctccattat ttagtgtttg
     2341 tggagatggt gcatttacga ggaagttagc aactggctga gaggaattgc ctgttgctcc
     2401 tgttggacct gttgggcctg ttactcctgt cgctcctgtt gctcccgttg gtcctatact
     2461 tcctgtcgct cctgttggac ctgttactcc cgttggccct atacttcctg tcgctcctgt
     2521 atttcctgtt ggtcctgttg ctcccgttgg tcctatttct cctgttactc ctgttgggcc
     2581 tgttgctccc gttggtccta tttctcctgt cactcctgtt gggcctgtta ctcccgttgg
     2641 tcctatttct cctgttactc ctgttgggcc tgttgctccc gttggtccta tttctcctgt
     2701 cactcctgtt gggcctgtta ctcccgttgg ccctatttct cctgtcactc ctgtatttcc
     2761 tgttgggcct gttgctcccg ttggtcctat acttcctgtc actcctgttg ggcctgttgc
     2821 tcccgttggt cctatttctc ctgttggccc tatttctcct gtcactcctg tcgctcctgt
     2881 tggacctgtt gctcctgttg ctcctatact tcctgtcact cctgtcgctc ctgttgctcc
     2941 cgttggtcct atttctcctg ttggtcctgt tgctcctgtt ggtcctatac ttcctgtcac
     3001 tcctgttgga cctgttgctc ccgttggtcc tatttctcct gtatttcctg ttgggcctgt
     3061 tactcctgtt ggtcctatac ttcctgtcac tcccgtattt cctgtcgggc ctgttgctcc
     3121 cgttggtcct atacttcctg tcactcccgt atttcctgtc ggacctgtta ctcctgttgg
     3181 tcctatactt cctgtcgctc ctgtacttcc ggttgggcct gttactcccg ttggccctat
     3241 acttcctgtt gctcctgtat ttccggttgg gcctgttggt cctatgcttc ctgtcgctcc
     3301 tgtacttccg gttgggcctg ttactcccgt tggtcctgta cttcctgtcg ctcctcttgg
     3361 tcccattgga cctgtaattc taattggaag acactcacat ttaggataac aattatctat
     3421 aattctatct tcaaatgtta caagtatata cggtttgtta caacctctac tagatgtaaa
     3481 ttcaagaatc tgataagggt aatttaaaga taaagctaac ccataattag gatatttatt
     3541 cattgcccat gcttttatag ttcctgtaat atttaaacaa atatagttgg atgtatcact
     3601 tatcccaact ggtaaatatc ccctaatatt ttccatagac actctatcat tccatgttat
     3661 agttcgtaaa ttataatatt cacttattct tttaatttca atatctactt tttctttaaa
     3721 aatatgattc ttatttgaat caatatgaat aaataactct gcttttaata tacttttgtc
     3781 ttttaatata tctggtattc tcactttaaa aaaagtaatc aacttttctt ttacaaaact
     3841 atttgaacat ttacttccta ttaagcaata atctaaatta ctaaagtttt tatctggata
     3901 ttttttagat ataaaagtat catcatttaa ataaagtata atatttctca tttataatcc
     3961 ctccattttg ggtctatatc atactatgtt taaaatacat gcttagttac aatataatta
     4021 aaatttatag tattaaattt ttatataata atgctgattt ctacaaaaaa agaacatgtc
     4081 attagaaatt tctaatagca tgttcttttt cattattata tattacactt taaattattc
     4141 cttgactggc ttagctggtg taaactaaac cagtctttct aaaatattaa gcactttttt
     4201 cttatta
//
LOCUS       DAELJO010000263         4201 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELJO010000263 DAELJO010000000
VERSION     DAELJO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.265"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            207..1940
                     /locus_tag="KPB88_003598"
     CDS             207..1940
                     /locus_tag="KPB88_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0055833.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2332..2755
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2397..2425
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(3035..3187)
                     /locus_tag="KPB88_003599"
     CDS             complement(3035..3187)
                     /locus_tag="KPB88_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055834.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
BASE COUNT         1637 a          567 c          568 g         1429 t
ORIGIN      
        1 tcttttttta aacttgtaca ttagtttata aatatgtatc tatacaagat attgacatta
       61 agtactgaag catatattgt ttcagttctt ttttgttaaa attaacttat ttaacaaaga
      121 tatcatagtt taacaaagct tttaatttaa atatattgat gtataatata attaaaatat
      181 aatagcaaaa agaaaggagg aatatcatga gcatactcct aaaaaaagca cccaaattag
      241 caaaacatat tataacaagt ttttatataa atcgggatat tgatgaagta ttaaaatacc
      301 tttgtgaaaa tgttacatgg atcggaccag gagaacagga atttctcact tcttttaatg
      361 aaataaaaaa ttatttttat gcaggtcaag atgaaattcc ttcttgtgat ataaataatg
      421 atatatttga aactgtaagt gaagatgaga atagatgtat ggtattagga agatatactg
      481 ttcgaactaa agaaaatgct caaatgattt tagaggtaaa tcaacgttgt acatttgaga
      541 taattgagga tagaggaaaa ttattagtaa agcacatgca catatcaaac ccttatggtg
      601 aaatgcaact tgatgaatac tttcccacta aaattggaac tcaaagttat gattatttgc
      661 aaagattact aaaggaaaaa acagaagtta ttgaaatgat tacaaataat atcaatggtg
      721 gcttaaaagg tagcaatgat gataatacat actcattttt ctatgtcaat gaaggattgc
      781 ccaaaatatt aggatacaca tatgatgagt tcatggaaat gagtggtggt tcagcagtgg
      841 gtgctgtata tccaccagac ttacctaaag cactagaaga ttgtgaacga tgttttgcaa
      901 aaggacctac atattcatct gaatatagaa tccgaaaaaa agatggaaca ttaatgtggg
      961 tattagattc tggaatgaaa tcgttaaata gtgatggcat agtaaaaatt aatagtatta
     1021 ttactgacat aacccaacta aaaaacatag aatctgaact aaaattagaa agagagcgat
     1081 atcgtattgc tttgcaaaat attacagata tcatgtttga gtatgatatt gaaaacgata
     1141 cccttataaa atatcaacgt gtagaaattg acaaaaaaat tgaattagaa aattttgaaa
     1201 caaaaaatta ctccaaacta ttagaatctg gtaaaattat tcaccttgat gatattggaa
     1261 aactactaga agtacttcat ggaaatcttc gtgaaacaat tgagataaga caagtaaatt
     1321 cattaactaa aaatgaatgg agatggattc gtgttcaatg ttcagttata tatgatagtg
     1381 accataaccc tatcaaaaca ataggcgttt taaaagacat aactgaagat aaatctaaat
     1441 tagaaagctt aattaatcag gctcaacgtg acccactaac acaattatat aatcaaagag
     1501 tatcacaaaa tcttattgaa gagtatctat gtagctctga ttccaaaaat aatgatgctc
     1561 tattagttat tgatattgat gattttaaaa ctgtaaatga tacttttggg catttagagg
     1621 gaaatgaagt tttagttgct gtatcaaaaa tactattaca taacacacat gataaagaca
     1681 tagttgctcg aattggtgga gatgaattta caatatttat aaaatcatta acaaaggatt
     1741 taataataaa aattactaat gatattttaa atgatgcgag taaaataaaa gtaaaggaca
     1801 atcataaaat aacattaagt attggtattg ccttcactga tgattcaact aaattatata
     1861 aagatttatt ttctaaagct gataaagctc tttatctatc aaaagcagat ggcaaaaatt
     1921 gttatagtac atacgaataa taaagctatt tatacgacca agaatgaacc tttaaattta
     1981 atttcatagt ctaacaatca aagagttgaa gctaatctta tagctagtca actagttcct
     2041 agaaaaaatt taaacaatat atttattgat actgctcatt tttctaaaga gcaaatttat
     2101 ataaaaaatt aaacttttat gtacatacag atactaatga taaacagtta tcattagtat
     2161 cttctatctt aaaacaaaat gataatttat ctatataaaa aagtgctttg ttgacagagt
     2221 tcactttttt atataggtac agaatttaag tataaatgca tgttttattc tatacatttt
     2281 agttaatgta aaatttcttg attgtactct ctaacttttc aacattatac tatttatata
     2341 ccacttggtt aatataaaac tattattata gtgtcgtgtg gagggcggtg tgacttattt
     2401 acataccact tagttaatat aaaacctggg agttgatgtt tcaacatatt ttactgctgc
     2461 attatttaca taccacttag ttaatataaa acaccctact gttatacttg ccacattaaa
     2521 agatttacat ttacatacca cttagttaat ataaaaccct gttatatcct gtttgcattt
     2581 ccataatttt ataatttaca taccacttag ttaatataaa acaatggaaa gcaggagacc
     2641 tattatgtag agttggtgga tttacatacc acttagttaa tataaaacgg gatatagggg
     2701 gagaatagcc atttcctaat tccctaattt acataccact tagttaatat aaaaccccaa
     2761 aataaactgg gcattttcaa tgcttacaca tgtgcaactc tcttaaattt gcagtgaacc
     2821 atgagtagtg caaatgataa agcttatcac atgccctcaa caccttatat ttcaagcgtt
     2881 aagccctact ttgtaacaaa tatcgctcac tgcaaaacta tttacatttt tattatatca
     2941 taaatatatt atttttgaat atctgtacca atttgtggta taataaaaat aagaagaact
     3001 acaatctatt tagatgtaga gtgaagttca aatactaatg tttattcttt ttgaacttga
     3061 tttttagttc aaaactaaag tcactcctgc cagagtggct ttttactttt ttaaataatt
     3121 tactatttaa gtaaactatt aaactggcaa ctaaacttgc tagtacacct tgtaaaaaat
     3181 tatccataca tactcacctc ccttctatac attgggagga taatcttttg tatgaactcc
     3241 actctataaa ttgtagatta catcttcttg ctaaaaatgt tatagcatat aattattaca
     3301 tattttacct atatattact tttctaaaga ttcgtctata tatttctgta tacatctact
     3361 ctatcctcga tttgttacaa ccccaatcaa aaaagtagtt caacttctaa gttttataca
     3421 aagcaggaaa ctcaattatt attagaaaaa tcaaaaaata ctcctatagg tataccaatt
     3481 tttttgatgc taactcttgg attacgtttt ggtgaagctg tatcacttat atggtcagat
     3541 gtagaccttg ataaaaaatc tcacatgtaa atcaaacatt agtttatgta tacaataaaa
     3601 taatcttcaa aggccctaaa acccctagat tcaaaagaag attatttgtt cctgatgaac
     3661 ttataagttt gcttaaaggg gaaaaattaa aacaaaataa gttaaaatta cagggtatat
     3721 tgaaaaatga atttgatttg atatgcttaa ataaaagatt caacccgtga gttcaaccaa
     3781 cttttgttgt tcattttaaa aaactattaa gagataataa tcctagagat ataaaagtac
     3841 atgaattaat acatgcaaat gcgactttga tattactttc aggtactaac ataaaagcta
     3901 tttataaaat attagtacat gcagatataa aaatagctat gaatatatat tccatattct
     3961 cacttattag atgaaatgaa taaagaaaca tctgaaaata taagcaaaat actattcaaa
     4021 taatgttcag ttttatgtca attaaatgtc actcaaagtg ctttttttcc atttgtcagt
     4081 taaatgtcac ttaaagtacc tttttcctat ttgtcagtta aatgtcagta atatataaat
     4141 aagatatatt tataagaccc ttaaaatcac tattatttaa taaaatttat aattatatac
     4201 t
//
LOCUS       DAELJO010000264         4175 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELJO010000264 DAELJO010000000
VERSION     DAELJO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.266"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            41..128
                     /locus_tag="KPB88_003600"
     tRNA            41..128
                     /locus_tag="KPB88_003600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Leu,seq:caa)
     gene            complement(315..758)
                     /locus_tag="KPB88_003601"
     CDS             complement(315..758)
                     /locus_tag="KPB88_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055835.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(761..958)
                     /locus_tag="KPB88_003602"
     CDS             complement(761..958)
                     /locus_tag="KPB88_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055836.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(1150..2016)
                     /locus_tag="KPB88_003603"
     CDS             complement(1150..2016)
                     /locus_tag="KPB88_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055837.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPN"
     gene            complement(2242..3111)
                     /locus_tag="KPB88_003604"
     CDS             complement(2242..3111)
                     /locus_tag="KPB88_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0055838.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3617..3838)
                     /locus_tag="KPB88_003605"
     CDS             complement(3617..3838)
                     /locus_tag="KPB88_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055839.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
BASE COUNT         1433 a          701 c          414 g         1627 t
ORIGIN      
        1 caaacaatat atttttttgc tataataata tacatgacat gcccgagtgg tggaattggt
       61 agacgcagtg gactcaaaat ccaccggact aatactccgt gccggttcga gtccggcctc
      121 gggcaccatt aaaaactcct aaagctattt attcaataga ttaggagttt ttttattttt
      181 gatttaaatt ttaagttatt ttttgaaatt actgtgtctt tttaaaattt atataattat
      241 ataaatttat cttctagcat taatttaaaa actaatttac ttttatataa ttttctattt
      301 tatatcataa cttattacaa ccttttatct aatttattgt atacaacaac actacaaata
      361 aattgaaata aaataagcaa tccaagaaca actaaaaccc acttgcttat taccattaca
      421 gaaaatacac tcaatataga aacaataaat ataagaatta caagaacata gcacatgatt
      481 tgattagtaa tacgtccagc tttatcacgt aacataattc ttctttcatc attcatctct
      541 atcctttgat tttttatctt ttcttcataa tctactgctc tttcaggttt actccaatac
      601 atatattgaa gaatcataca tacacctgga cctaaagcag ctccagataa tccccaaata
      661 aaagcctcaa atgaaaactc agtaaatgta gccaataaaa caagtaaaat tccacaaata
      721 aaatatataa ttccaacata taacatattc tttttcattt ttactcctcc tcataaataa
      781 atatttcttc aatactcata tcaaagtatc tagctatctt aaaggctaag atgatagaag
      841 gattatacct accattttct aatgaaccta tagtttgtct tgatacttgc aaaatagaag
      901 caagttcttc ttgttttatg cctctttctt ttcttatttc ttctaatcta tttttcacat
      961 ctatccccct ttaatatctc aactatggaa agtctacttt ccataattat acaactatat
     1021 tccatttatg taaagcttac tttccatatt tacaaaaaaa taacagacta ttttcagcct
     1081 gtaattttag aatttcatgt tgtttattaa ttttaatgtt atttattaat tccatattat
     1141 ttattaattt taatttggtt tctgaattgg aatccaaact tctgtagtat aatcaggagc
     1201 attgatatcg ccattgggat aaagttcaat atctggtaaa ttagcataca catacccaga
     1261 acttggtaac cactcagtaa taattctttt ttgcaattcc tgtattgcca gagcatttgg
     1321 cattggagaa acacattcaa atactgccca tgtgcctgat ggtattaagt attcatgagt
     1381 atcttctaat acaggcttat ctgttggagc agcaatataa taatctaaac ccttactatt
     1441 tgtacacaca ctaactccaa gcaatccaca aggttcgtta tttattaact ctgaaagttt
     1501 atcaattgtt ccatttttta tgcatttagc ccataactta ggaactttct caaaacactc
     1561 ttctacaatc atgttcatac tttcttttat accaacaata cgaaaccctt tcttttctac
     1621 aatacgatat tgcatttcta tatttccttt tacataaatg cttaatgtca tcttaggata
     1681 tgcttttaaa acaacaccct ttgctctagc tgcagatgga gaaatgttat gtatattttg
     1741 aaaagctcta ttaaaagatg ttggtgaatc gtaaccatac ttctcagata gttctaaaat
     1801 tttgatgtta ctgttctgaa gttcaaatgc cgccttagtc atacgtcttc gtctaatata
     1861 ttcagataat ggtacttctg caatatatga aaacattctt tgaaaatgaa aggttgaaca
     1921 acatgcaatt tttgctgctt ctgcataatc cactccactt tcgagatttt cttcaatata
     1981 agtaatagct tgatttaatt tatttatcca ttccatttga tattacctcc aagcaaatta
     2041 tacagtttaa taaaataata tacctctcta ttcatgcaca aaattgtaag catttaaatt
     2101 cataagtatt tttcaatcaa tacaataaat ataaaaattg tattatacaa taaaaaagtt
     2161 caataacatt atattgtaaa atgttattga actttttata aaaattaatt taagtgtagt
     2221 taaattaagt attacactat cttatatatt acaatatcct actatcttat agtcttctgg
     2281 cactttataa tctttgtctg gagtacctaa attccatttt aaatcaaaca tagttgcact
     2341 tattattgtt gcaaaataaa gcataactat accaatgtct attttttctt catataaatt
     2401 agtatgacca tcttttctaa cagctaaaac tacagtgcct ccatcaacta taaatctcca
     2461 tggttgtcta tttaatgtag atggagctaa tcttgcatag tgaaatgcat caagaagtgc
     2521 tctttcttct aagatttcta tacttgcaac atttccccat tcgttcatat aaacaacatc
     2581 ttctacacct agtctagtag accccttatc tgttacaact tgcatatcag attttgaata
     2641 attttgccca gtttttgtag catttaatac tttagcatta cttacatttt ctccatatcc
     2701 aatagctata attcctgtta cctctttatc tgaattaatt ccaagttttt cttttacctt
     2761 ttttccatct tcaaaagtaa cccaacaaga accaattcca agttctgtta ctttaaagat
     2821 tatactttct cctatgtacc ctgaattttc aatgtaatta tcttttatgt cagataatat
     2881 tataatataa cttggagaat ctatcatatt tcctttgtac cctgctactt tatctaaatt
     2941 attaaaaact tcacttctat tcataattct tacatcaaca tctatttctg gaactagttt
     3001 cttacaagat tctgagtatt tcttaatttc actaagatat tcttctttaa tatctgtgtt
     3061 cttatattct ctaacagact ttctatttaa tatgattgtt ttataatcca taattttatc
     3121 ctccccaaac tagtattgtc ttaattatat acccattaat atattatttt actcatttat
     3181 ttaataaata aagattacaa ttttgtaatt attatttatt aataatattc cacttttaca
     3241 ttaataatga ccagttgtgc aaaatccatt gccaaccacc aaataaaaac atattataaa
     3301 ggtaatataa aataacaaac aaatcttaac aaaaaattaa acttttttta cacgaaagaa
     3361 ggcttcccgt tgccttcttt atttttataa aaaaataagc tgtttctaaa atgatttcac
     3421 atttttgaaa cagccaagtt attctataat gacaactata atttgaggat gtgagggaga
     3481 gttgtcatta taaatttagg gtaattatat tatgttttaa gctagttctt tctatattta
     3541 tataatacat tttatatatg aattttatat gagattggta taaatgatat atgaaattac
     3601 atatgtttat attatattaa attttattat tacattttcc acaacaatac tcatatgtct
     3661 ccacattatt tggaatattt acaaatacaa attctttaca gttctcacaa tactttaata
     3721 tagatatagt tccacaagtg taacattcaa atattacaca atcctcattt tcctctacta
     3781 tttcaaactt tttactatgt tcaccacact ctgggcactt gtgattattt acattcataa
     3841 atactcctcc ttattagtat agttacatgt caaaaactta attaatgtat tctatattac
     3901 ttattatata ttctataata aatcatattc taatataata aaacaaatta aataaaaaaa
     3961 aaccataaat tttaaattta tggattaatt cttgtaatga taactctatt tgaggatgtg
     4021 agggagggtt atcattacaa tagggtatta tattatggtt tttaagctag tctttctaca
     4081 cctcatatga tacatatgct atatgaattt tatatgattt ttacataaat attttatgaa
     4141 atttaaattt tttagtcatt ttgaaatttg taaaa
//
LOCUS       DAELJO010000265         4171 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELJO010000265 DAELJO010000000
VERSION     DAELJO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.267"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..297)
                     /locus_tag="KPB88_003606"
     CDS             complement(<1..297)
                     /locus_tag="KPB88_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055840.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIAL"
     gene            complement(415..2238)
                     /locus_tag="KPB88_003607"
     CDS             complement(415..2238)
                     /locus_tag="KPB88_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055841.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSMIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2251..4026)
                     /locus_tag="KPB88_003608"
     CDS             complement(2251..4026)
                     /locus_tag="KPB88_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055842.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINASIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1357 a          737 c          368 g         1709 t
ORIGIN      
        1 aagagctatt ctcttataag cttctcctac ttctggataa ccttctctat cagcttgtct
       61 actcattgct aaatacattc ctacttctgt acactcacca acaaagttag ctcttaatcc
      121 ttcgattatt tcttcatcta caccttgagc tactcctatt ctgtgctcat cagcccaatt
      181 tttttcgcct ttcatttctt caaatttatc agctccaact ttacatactg gacattgtgc
      241 tggtgcagca tctccttcat gtatatatcc acatactgta caaacaaatt tcttcataat
      301 tactttcctc ctaaatttta tatatcaaat taaatttttt atttattaag ttagtctata
      361 tatacccgca ccatatacct ttaaacaatt tttttaaaag ttttttctaa atacttattt
      421 tccctgtgtc aataatatgt ttctataata atgtcccttt aattttatca attctttatg
      481 atttcctgat tccacaattc taccattttc taaaactatt atattatcac aatttgatat
      541 ggtacttaat ctatgagcta taatcatact tgtctttcca taagttaaat tatttattgc
      601 ttcttgaatt ttagcttctg ttgctatatc tatgttactt gtagcctcat ccaaaataag
      661 tattggagca ttttttaata ctgctctagc aatacttatt aattgttttt gaccttcact
      721 taaatcttca cccctatccg ataatacagt actatatcct tcacgcattt taactataaa
      781 tttatgtgca tttgctattt tactagcata tataacttct tcatcagtag cctccagatt
      841 tccatacctt atattttcca taactgtatc attgaatata taaccttctt gtggaactac
      901 tccaaaacaa gttctcaaat agtttctgtt aatagagtag atatccttac catctaaaaa
      961 tatacttcct tcatctatat gataaaaatt tgtaattaga tttataatcg ttgtttttcc
     1021 acttcccgtc tctcctatga tggcatttga tgtgccagct ttgattttta ggtttatatt
     1081 atctaaaatt ttcttttttg aatcataaga aaaacttaca tctttaagct caatttctcc
     1141 ctttacaaaa tgagagttta aattaccatc tttattatcg ttaatctcac ttttaaacat
     1201 gttatgcact tcttgctctt cttgttcatc tataatttca aaaactcttt cacatccagc
     1261 tatggctgaa agtaatgtat taaatagatt tgctaattca tttaaaggtc ttgtaaattg
     1321 tcttacataa cttaaaaaac tagctataac ccctatggtt ataatatctt tagatgcaag
     1381 aatgcctcct ataatacata tagttgcaaa tcctatatta ctaatcacat tcataatagg
     1441 cattaaaaga gaagtgtaaa tttgcgattt aagggaaata tttagaagtc tatcattgac
     1501 ttctttaaaa tcatttacag attttctttc ataattaaaa gattttacta catttatgtt
     1561 tgatatcatt tcttcaatat gtgcatttag ttttcccaac tcaacttgtt gttgcttaaa
     1621 aaaaactcta gttctctttg caataaaatt actcacaaac attactatcg gtattaatat
     1681 tacactgcaa aaggttaaaa ttgggcttaa tacaaacatc ataataaaag tcatcaatat
     1741 gtttaagatt cctgtaatta actgtactaa tgaatttgat attcctgtac tgatattatc
     1801 aacatcattt gtcactctac tcataatatc tccagtagaa cttttatcaa aaaaggttat
     1861 tggtagattt ttaaatttta aaaataaatt acttctcatt tctctgacta tactttgaga
     1921 taatctagct gtacatatcc ccttagacag tttaactgaa aactcaatta tataaaatga
     1981 aagtaaaata attattcctt ttaagaaaat ggattttgaa taagtatcga tatctattat
     2041 gtcaactacc tttccaatag tatatggtat tacagttgat ataattgaat caattattac
     2101 tagcaaaaaa ataaatctag taatcttctt atctttttta aagtacataa aaattctttt
     2161 aaacgttttt ttaaaatctt taggcttttc ttttttcttt aatctactga ccttctgatt
     2221 tccagtactt aaattcaata tacttcctcc ttaatttaca tcttcactta ctaaattttg
     2281 caacgcaaaa atttttttat aaattggaga actaaccagt aaatcttcat gtgtagcaaa
     2341 tccaaccacc tcaccctcat ccaagactaa aatcttatca cagtcaatta tagaactaat
     2401 cttttgtgaa atagttatta cagtacattt cttactttcc ttttctctta aattacttat
     2461 atactctttt atattctttc ttataattgt ctctgtatta atatccattg cactaaaaca
     2521 atcatcaaga attaatattt gtggtttttt ggctatagct ctagctatag taattctttg
     2581 tctctgtcct cctgataaat ttttacccct ttggaataaa acttcatcat aagtattttc
     2641 gagttttgtt ataaattgat ttgcatttgc tatttcacat gattttacaa catcctcttt
     2701 gcatatatct ctatttccca taactacatt ctccaatata ctttttccaa acacactact
     2761 attttgaaat actacaccta ttctttccct taaactttct tcagaaatac tgtatatact
     2821 acgtcctcca atcttaatat cacctttatc aattatatat aatttttgaa ttaaatttac
     2881 taaagaagtc tttcctgaac cagtttgacc aatcacacca atattttgac cttcttctat
     2941 tttaaaggat acgtttttta atattttttc atctgtattt ttgttatacg aaaaatctac
     3001 attttcaaaa attatatcac aatttacaaa ctttaaatct ttttctccaa catctaaact
     3061 ttcttctctc tcaattactt cttcattctc atcaaccaaa acctcactta ttctctcata
     3121 agatgctttt gccctaacaa aaacattaaa aacatgtgaa atcattataa gagatgttaa
     3181 tattctagtc atatagttta cataagcaac tataatccca attttaattt ctcctgaata
     3241 aattaaatgc cttccaatca gtaatattat tattatggaa gcattaatga ttattgtaat
     3301 tgtcggtcta aaaaatgaca tcatagttgt agcttttcta ctatttttaa aaagttcatc
     3361 atttaactct tcaaagcttt ggttctcata atcgtatcta caaaatagtt taactacttt
     3421 tattccactt aaaaactgtc ttaattttga atttacttta tctgtacttt cttgtacatt
     3481 tttaaataat ctatacccta tcttcatatt aagcaatatt ataataaata taattaaaac
     3541 tgcaattaga ataattattg acatctttaa atttaatttt atagctataa ttaaacttcc
     3601 tatacacatt attggtgact ttataaaaac tctcatcatt ccattaacga attgttgcat
     3661 ttgattgata tcattagtaa ttcttgttat taatgaagct ctaccaaatt tatctatttg
     3721 ttttaatgag tatgtattta tcttttcaaa tacatcctgt cttaaatcat aagaaaaatg
     3781 ctgagaaact cttgccgaga ttacacttct tattattgca aatattgcac ctaaaaaagt
     3841 tatacctatc attatagctc ctgttgatag tatgtagtct aagttcttat ttgaaacccc
     3901 tatatctatt atttttgcca ttatagttgg ttgagctaaa tcacaaaaag cttccaatgt
     3961 taaaaaaaat actgctgtta gaaataatac tatatatttt tttatgtatt ttttaaaata
     4021 cctcattcaa tcatcccttc atagaatttt ctcattacca aaagtatatc ataatggcta
     4081 atttaattga ttatatcaat tataaatatt tgatttatta ttaattttta taccatatat
     4141 tttataaatt atttgttttt atttcctata t
//
LOCUS       DAELJO010000266         4168 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELJO010000266 DAELJO010000000
VERSION     DAELJO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.268"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..226)
                     /locus_tag="KPB88_003609"
     CDS             complement(<1..226)
                     /locus_tag="KPB88_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055843.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIH"
     gene            complement(257..1276)
                     /locus_tag="KPB88_003610"
     CDS             complement(257..1276)
                     /locus_tag="KPB88_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG0055844.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(1294..2247)
                     /locus_tag="KPB88_003611"
     CDS             complement(1294..2247)
                     /locus_tag="KPB88_003611"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG0055845.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(2589..3365)
                     /locus_tag="KPB88_003612"
     CDS             complement(2589..3365)
                     /locus_tag="KPB88_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0055846.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(3399..3920)
                     /locus_tag="KPB88_003613"
     CDS             complement(3399..3920)
                     /locus_tag="KPB88_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055847.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
BASE COUNT         1278 a          736 c          489 g         1665 t
ORIGIN      
        1 catgtatatc aaaatttggt atgtcaaaat caaattttgt tatatcaaat ttaatacttg
       61 gaaaatcata ttgtttcatt cctagcatac tttttgcatt actgtctact ataaatacac
      121 aaactgatgt acctataatc atagtaatca aaaataagat tctcataata tacttgtatt
      181 ttcttagttt acttttactt tctatatttt tttctaatcg gctcatttag catgctccta
      241 attataactt aaagctctaa tttttttgtt taaaatattc atccaaaatc tcagctaaat
      301 actttgtagt agccttagat tcttctacac ttgttgcgtt attccctatt tctactaaca
      361 tatgattctt aactgcaaac tgattgaatc tagcatagtc tttttctact attggagata
      421 ctagaccagg atactttttc tcagcaaaag cagttatatc ttctgctaaa gctctaagtt
      481 gttttctatt tgtattttta cctcctacaa caaagaagaa ctttgatact ctctctccat
      541 ttatcatagt tgtatatttt aaatggtctt tttccttagt ggctggatta gttaaatccc
      601 tagcatctct atgtaagtct atagctatat ccactgagtt atatttaggt aatatacttt
      661 gtattgtttt taagctactt gcataagagt tattataaga tggatagtca tggtatttag
      721 tagtatggac tacaccccat cctttgctat ctaaagcact agttagtgca cttcctactt
      781 ccattactga atttttctta tctcttgaat gataatttcc atcaggtgaa tttgaataag
      841 tctcacatcc atgagtatga tatatcaaaa ttcttggatt ttctttggtt actcttaaat
      901 catccttata atctgaacta gatgttgtgt tagtttcagt tgtgtctgaa ttttttgact
      961 caatatctgg aacattttct ttaccaacat aaagttttat atattctttt tcactctctt
     1021 ttttattatc gactttactt gcatctttag atttagtgtt tccttcttta ctttcttcct
     1081 tattttcctt acttgtttct ttatttttat tattcttttt attttctgta tcatttcctt
     1141 cgactttagc ttctggataa gatgaattta ctaaaaactt taaaaaatca tcttgattca
     1201 aagctaatga ttttcctggt aaaatgcata ctaatataaa agtcaaggtt actactttaa
     1261 tgcatttttt aaacataatt gctcctcctt attttatacc aatctacctg gatgtaaaga
     1321 cctatttaag ccttcactta ttattataga tagattttcg attgtatcat ctatatcctt
     1381 tggtgttact attaagttct tgtcatatgg gtctaacgaa tcttttatta gatgatattt
     1441 ttcttcttct ttaagttttt tcaacatttc ataaaaactt tctgtttctt ctgattgagc
     1501 tattaaattg tctattgcca tatctaagac atctattgta agagttgctg catcaaccac
     1561 cgttggaacg cctattgcaa taacatccac gcctaaagtt tctttagtta gagattttct
     1621 agtatttcca acacctccac caggtgatat tcctgctgtt gatatctgta tagtagagtt
     1681 tactctctcc atttttcttg aagccaatgc atctattgct actactctat ctggcttaac
     1741 cttctcaacc aatgacttta ctatttcact agtttctatt ccagtaacac ccataactcc
     1801 tgggcttaaa gctgatacct cagtgaaatc atcatcatag tctttattat aatttttaaa
     1861 aagatgtcta gttacaagtg tttttgatac actctttggt cctaacgcat cagaagtaat
     1921 atttctattt ccaagaccaa ttaccagggt ttttttattt ttgtcttggc caaatatatc
     1981 tactaactcc tcttttaaat aacttatcat ttcttctctt gactcatcat catcaaactt
     2041 cataagatta ctttccagag ttatatactt tcctatatct ttattcatta tctctgcgcc
     2101 ttgcctgtct attacttcaa ctttagttac aatgcaattt tctaattctt tagtttctat
     2161 ttttacacca ggaatactac ttccttcttg agacttttca cacatttcac tagcttctaa
     2221 agctaaatct gttcttacac taatcatata aacctccagt ataaattaaa tgctaattat
     2281 aattgttaac aaaatttagt attttataac aagaaattgt aaatttattt aaaacaagta
     2341 aaattaaggc tttcaatgta agaaaattgg gtttagtcag tgatttgtca gtaaaccttc
     2401 ttatctatca tttatttaca aacagaaaaa ggtagcaact atttatatag gcgctacctt
     2461 taatctattt ataattttcc tattaagctt tcactaatat attaactaaa ataatttttt
     2521 atatttatac ctaatttatt tactatataa aaaaacagca ataattatac aataatcacc
     2581 taacaccttt attccttttg gaaaaatggt tctgcaattt ccaaccattc atttggaagg
     2641 taaagtgata aatacccatg attatagctc ttagcttcat aaatctcaca agagggcaca
     2701 taagactgaa acatttttgc agattttttg acacacttca tttctttttc tccataccag
     2761 tacattactt gtgctgtggt ttttttaaca ttctctttaa gtgtatagtt catcatataa
     2821 gtacgataca tattatagaa tgtttctttt cttacatgtg gcatatctgt catataatac
     2881 gcttttattt catctggata ttgcatattt tcaggtaaca attttggcat catagcaatt
     2941 tgaaagcgac aagccttctc actaaacaaa agtcctccaa aaaatcgaat accagcaata
     3001 caaaaccttg ccataagagg tcttgggtag caaatactac catcaataat tgctttttta
     3061 gtaatattag gttttcgaga taaaagttcc atgacaattt gaccaccaag tgatacccca
     3121 cacaaagcga aaagatgtcc actgcaattt tcatctatat atgatatcaa cttatctgct
     3181 gtatcctctg tagaaatgta tgttgtttca tattcttctc catgaccatc caatgtagga
     3241 agtatcacat gatatttgtt agacaaaact tctgcttgtc gcaaatagtt ccaccatgca
     3301 ttccctcccc catgaatcaa cataatatgt ggtctgtttt tatttccaaa ctcatgaaat
     3361 ttcatatata acctcctttt ataaaaatgg gttgcatgtt attttgtttt atcattatca
     3421 gctacaggtg gagaagatat aattgaaagt tttctaagtt ttagatttga tttaggcttt
     3481 tgttctatat atggtttcaa taaatcagct atacttttaa gaagattttc atattcatta
     3541 tcactcatat atagtaatgc tgaagagaaa aatacattat cctttttatg gtcttgctct
     3601 tttctgctaa agtattcatt catttcctgc atcagaccta cataaaatgc tgtagaaagt
     3661 gcaacgaatg tctctccttc catagatgtt gtatattcct ttagtgcata aaccttttca
     3721 atagtaccac gcacacgatt ttctttaatt acttcaatca tattgttctc ttctaaaatt
     3781 tttacatggt gataaactgt tgcacgagga atatcattta aatactttac aatatcagat
     3841 gtgcgaacac tgctatgaag tcgaatatat tgtaagatac gaagccttgc aggatttaaa
     3901 attagttttg ctatgtccat cttatcagcc ctttctttag tctataaaaa tattatagtc
     3961 tattttttta gactattcaa gtctatgata taagtagtaa ttacaactat aataaatgct
     4021 acaaatgcta ttctatataa acactgagat taattacata taaccttcta acatattagt
     4081 tatattgttt gatgcatcta tcatacatac taatgaaaaa aattatcttg attattggaa
     4141 aaattatctt aattaagcat ataaattt
//
LOCUS       DAELJO010000267         4151 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.269, whole genome shotgun sequence.
ACCESSION   DAELJO010000267 DAELJO010000000
VERSION     DAELJO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.269"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            202..867
                     /locus_tag="KPB88_003614"
     CDS             202..867
                     /locus_tag="KPB88_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG0055848.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            889..1821
                     /locus_tag="KPB88_003615"
     CDS             889..1821
                     /locus_tag="KPB88_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG0055849.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1842..2483
                     /gene="pcp"
                     /locus_tag="KPB88_003616"
     CDS             1842..2483
                     /gene="pcp"
                     /locus_tag="KPB88_003616"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG0055850.1"
                     /translation="MKILLTGFDPFGGEPINPAQETVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2603..2935)
                     /locus_tag="KPB88_003617"
     CDS             complement(2603..2935)
                     /locus_tag="KPB88_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055851.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3201..3821
                     /locus_tag="KPB88_003618"
     CDS             3201..3821
                     /locus_tag="KPB88_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0055852.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         1564 a          556 c          629 g         1402 t
ORIGIN      
        1 atattagctt aaaatataat ctttgttatt caaattccta tacacaacca ttatttactt
       61 tgatacttta aaacatcttc cctatttttt attaaatttt ataataatag tcctattaac
      121 gcaaatacta taaaaagagt ttaactttat ccattaaata aatttaaatt aaaacatata
      181 gaaatggaag gagaacaatt tatgataaaa ctaatcggta tattgatagt tgtaattgga
      241 tttatactta aaattgacac attatttact gtattattag caggagtggc aacaggaatt
      301 gttgcaggat tagattttaa tcaaattttt acaattttag gagattcttt tgtctcaaac
      361 agaggtgtat cattattcat attaacattg ccagtaatcg gtgtattaga aagatatggt
      421 ttaaaacaaa gagctgtttc tttaatagaa aaattaaaaa gacttacaac aggaaaagta
      481 cttactatat acatgatagc tagacaaata gctggtgctc tatcaataag aatgagtgga
      541 catcctcaat ttgtaagacc acttgttaat cctatggcac aagctgctgg tttaagtaat
      601 agtgatgaac taaaagaatc ggatgaagaa gccataaagg cattatctgc tgcatcagaa
      661 aactatggaa atttttatgg tcaaaactta tttgcaggta gtagtggagt attacttata
      721 gcatctactc tgactcaatt tggatacaat gtaactggac ttaatatcgt aaaggctagt
      781 actattatgg cagtaatagc acttgtagtg gctacaatcc agtttacctt atatgataaa
      841 aaattaaaca aaagtcataa aaaatagttt tatttaggag gaataaaaat gcaaaatttt
      901 ataaacatat cattagaaat cttttacgca ctaatgggat ttttaatgat tgttatagca
      961 tataagtctt ttactaccat aaataacaat aagaaatatg gtacatcatt attttggata
     1021 cttatatcac tgccattcat atttggtaga ttgattccag ccaatataat aggaataata
     1081 ttaatattat ctagtttatt aacactttct aaacaagttg tatttgcaaa gtatgaagaa
     1141 ccagatgaaa attttggaaa agaacaagca gacaaactta aaaataaaat atttatacca
     1201 tcattaatat tagcatttgc tgcagtagta gtagctatga gcctttctaa ttttgataat
     1261 tcttctcaat ttgcaatagg tgtgggttca ataattgcat taatatcagc ccttataata
     1321 actaaggcaa aacctgctac atcagttcaa gatggctcaa gattacttca acaaatggga
     1381 cctgctagta tgttaccaca acttttagtt gcattgggag ctttatttac tcaagccgga
     1441 gtcggtgaag ttatttcaac catgatatct ggtgtagtcc ctgctgatag cagattattt
     1501 ggtgttatag cttatgtatt aggtatggtt atatttacca tgataatggg aaatgcattt
     1561 gccgcatttt cagtcataac ggcaggaatt ggtattcctt ttgtattgtc tcaaggtgga
     1621 aatcctgcta taattggagc attagcatta acagcaggat attgtggaac tctacttact
     1681 ccaatggctg ctaattttaa tatagttcca gcagccttgc tagaatgtaa aaatgattat
     1741 attgttataa aatatcaagc tccagttgct ttggtattaa ttatagccca catattagta
     1801 atgtactttt taggatttta atataattag gaggtaaaat aatgaaaatt ttgttaacag
     1861 gatttgaccc atttggtgga gaaccaataa acccagctca agaaacagtt gaaagggtca
     1921 ataataatat aaacggtgca gaaatcataa agataactat accaacagtt atgacaaaat
     1981 ctgttgaagc tatagataaa gctattcaag agcacaatcc agatatagta atatcagttg
     2041 gacaagctgg aggtagattt gacattacac ctgagagagt tgctataaat atagatgatt
     2101 ttagaataaa agataatgag ggtaaccagg ttatagatac tattataaaa gaagatggtg
     2161 aacctgcata tttttcaaaa ttgcctgtaa aagctatggt aaaacatatg aatgaaaata
     2221 aaattccagc tagtgtttca aacactgcag gaacatttgt ttgcaatcat gtgatgtatg
     2281 gaattcttta tatgatagac aagaaatatc ctaatataag gggtggattt atacacatcc
     2341 catatacaac ttcacaagta atagataaaa aaaatacacc attcatgtct ttagaagaaa
     2401 tagtaaaagg cttagaatta gctatagaag cttgtattat atataaagaa gatgtaaaag
     2461 aaattggtgg tgaaatttca taataaattt tttagtattt ttataaatta aaaatactaa
     2521 ctattaattt aaatatagac tgtgagtaga ttattcaaac aattttactc acagtctttt
     2581 tatggtatct acagatacaa aattaaggtc tattcctctc tccccactca cacattgcat
     2641 ctaaaattgg aatcagagat tttccccttt ctgatagact atattctacc ttaggaggta
     2701 tttgaggata ttcagtacga ataatcaagt tatctgcttc taattctttt aatgataaac
     2761 tcaaagtttt ataggagata gtatttatat aacgttttaa tgcattatat cttacaactt
     2821 caaattctac aagacagtaa agaattatca tcttatattt accactaatc agtgaaagtg
     2881 tataactaaa tcctgtttca cttaaatctg tatttgatat acagtctata ctcatatatt
     2941 acactctcct tttggttagt attgaacaaa aatgtgcgta cttgttttta gaataattta
     3001 taataaaatt atagtgtaag aataattgaa agtcaatttt ataataggag gtgtatttac
     3061 taaaaaaaaa gttattttaa ataggattct aagattagac atttctatat aaattgataa
     3121 tattcatgaa aatcctattt tataaaaaat aatgtctctt agaaaatcta ttaaataaaa
     3181 ttcattggag gaaatttata atgaaagttt tattgataaa cggtagccca aaccaatatg
     3241 gatgtactta tacagcacta aatgagatta ctaaagtatt gagtaaacac gatattaaaa
     3301 cagaaatact gtatttaggt aaagaaacaa tcccaggatg tattagttgc gcatcctgtt
     3361 ttgaaactgg aaaatgcatt cgaaatgata aagttaatga attgattgaa gatctggata
     3421 atattgatgg tattattatt ggttctcctg tttacttttc tagtgcaact ggtcaattaa
     3481 cttcttttct tgaccgtcta tttttcatag ctggaagtcg catggctaca aaattaggtg
     3541 cttcagttgt gtcatgtaga cgtggtggtg caagtgcaac atttgaccaa ctaaataaat
     3601 atttttcaat cagcaatatg cctattgtat catcacagta ttggaaccag gtacatggct
     3661 ttactccaga ggatgttatg aaggatgaag aaggattaca gactatgcga actctaggag
     3721 aaaacatggc atggcttctt aagtgtatat cagcaggtaa aaaagctgga attaaagaac
     3781 cacaatatga agaaacaatt atgactaatt ttataaaata aataaaaaat gtactaatat
     3841 taattaaaac aaaacactaa tttataaata aaataagcat aaatgtagga taaaaagcac
     3901 taaaacagag gtattgaatt ttatatcaaa cctctgtttt attattacat taataaccaa
     3961 accatatata acatcatata tttctaaata aattataata ttatattcta atgttattga
     4021 aattacaata actgaatttc tgctcataaa tttaaatatt ctacaaatct catctaaata
     4081 acaattttaa tagacctaaa acaatcctca tactgttcat tttgtattaa aaatatcact
     4141 tacaatcaaa a
//
LOCUS       DAELJO010000268         4101 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELJO010000268 DAELJO010000000
VERSION     DAELJO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.270"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     repeat_region   571..2233
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=571..599
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            2344..2628
                     /locus_tag="KPB88_003619"
     CDS             2344..2628
                     /locus_tag="KPB88_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055853.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(3399..3614)
                     /locus_tag="KPB88_003620"
     CDS             complement(3399..3614)
                     /locus_tag="KPB88_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055854.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(3625..4092)
                     /locus_tag="KPB88_003621"
     CDS             complement(3625..4092)
                     /locus_tag="KPB88_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0055855.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1573 a          416 c          561 g         1551 t
ORIGIN      
        1 gttatttttg cacttattca atttattttt gtgaaacctg gaaagataaa actttaatag
       61 cagataataa ccttgagatt gtaaagtaac aaagtgaaaa tgtataaaga ggaaaagaaa
      121 tggaaggaag aaaagtatat agataaagag atataacacg tattttgatt taactgtata
      181 agatgaaaaa tttgatgatt ttgaataatt taaaaaattg ttaatgaaaa taaaagactt
      241 attgagctaa ggtgcgatta cataggaagg atgcaataaa aagaaaaagg aatcattgga
      301 atagagaata taataaatac ttataagaat gtagatgctt ttagtttgta aaattatccc
      361 atttttattt tatagtatga gttttatgat ataataaaaa tataaaagtt ttgcagtgag
      421 cgatttttgt gataaagtag ggtttaatag ttgaaatata aagcgttgag agtgtatgat
      481 aactgttatc aattgcacta ttgctcgctc actgcaagtt taggagaatt gtatatgtat
      541 aagtattgga aatacttaat ttattttggg gttttatatt aactatatgg aatgtaaata
      601 atcatgcaac agtacgaacg tacaaaccat caattagttt tatattaact atatggaatg
      661 taaattttct atatcttgaa aattttgtta atgatgttgg agttttatat taactatatg
      721 gaatgtaaat cctgttgtaa ttcaagcggt cagaggaata gaaataagtt ttatattaac
      781 tatatggaat gtaaatctgc gacggaagca tataaaactc aatgtttaaa atagttttat
      841 attaactata tggaatgtaa atccttgaca caacagaata aacataatat ataagcatag
      901 ttttatatta actatatgga atgtaaattt atttctttca aactactatt ttcatttaac
      961 tttgcgtttt atattaacta tatggaatgt aaatctttta actgaaagaa tagtaatatc
     1021 aacatacaat gttttatatt aactatatgg aatgtaaatt tatcattttt tccgcttctg
     1081 cttgtaattt agactcgttt tatattaact atatggaatg taaatttccc taatgctcca
     1141 ttttcattat aacctggtgt agttttatat taactatatg gaatgtaaat gtaggtgtcg
     1201 gagagctagg aaactatagc ataatggttt tatattaact atatggaatg taaatcgttc
     1261 ttggaatgct aaaactcgtc cttcttggaa taagttttat attaactata tggaatgtaa
     1321 attgatttca ttaaatgtgt atttgaaaat ttctacaaaa gttttatatt aactatatgg
     1381 aatgtaaatc tggttattgt ctcatcacca ttatgcttag gtatggtttt atattaacta
     1441 tatggaatgt aaatctatat gcatctgtat aagtttgatg tatttcatca gttttatatt
     1501 aactatatgg aatgtaaatt aagcctctca caacatcttt cccgatactc ataatgtttt
     1561 atattaacta tatggaatgt aaattttcaa tcattgctat agagctgtta acaatatgtg
     1621 gttttatatt aactatatgg aatgtaaata ggaacaaatt gctaaaaaat taggggttgg
     1681 ttattcgttt tatattaact atatggaatg taaatctgag aaagttttat tcttttttaa
     1741 tgttacaaat cgttttatat taactatatg gaatgtaaat aaattattcc ctaaaaaaag
     1801 tttatttctt tctactagtt ttatattaac tatatggaat gtaaatatta cagtttcttt
     1861 attttttatt attgtacttg gcgttttata ttaactatat ggaatgtaaa tatagaaaat
     1921 ggcgataaac ctgttcgtcg attagatagt tttatattaa ctatatggaa tgtaaatata
     1981 agatacattt agtttatcta tctctttttc aaaggtttta tattaactat atggaatgta
     2041 aatttttcct cttctcgcac atggtcattt attacattgg gttttatatt aactatatgg
     2101 aatgtaaata caataccccc ctattgcaat gcttcattaa ctattagttt tatattaact
     2161 atatgtaata taaatgtttt ttcttattaa ataaagattg agtcacttta tatttaataa
     2221 tacatattct aaattgattt tgtttttata taagagaatt tgaataatat ggaaaaagat
     2281 gattaataaa ttattaatta gggtataaaa tgatataaat agaataaata aggggtggat
     2341 gaaatgcttg tatataataa aagtttttat cctaatgaca tatttccaag attagatttt
     2401 tcaaaaataa aaaaacagtt aaaattgata gataatgacc tgtcagattt tggaagcata
     2461 tgtataatag aaaaagaaca ttatacaata agtgtaaaca gtataggtga aataaatgtg
     2521 tattatgatt tagagtacga aaataaggtg tatagaatag tttatgagat tgaaaagtta
     2581 tttaaatctc aagttggaag gtttagcata tctacataca gaaattgata attaaaaaag
     2641 tagaaattaa aaaacttaat actaaagata tagataaata tatgaggtaa cagctagaga
     2701 gagttgttac cctttttaaa tactaagtat atattattct tattaatttt ttgtaaattc
     2761 actaaaagac ctttttatag aatattattt tcttttttta ttaaataggt aaatcagtaa
     2821 aaatgcaatt ccagttccaa ttattattcc aatgatattt tctataatta atttttttaa
     2881 tataaaagta aataataaaa atgctataca taaacctaaa atatatcctt taaaataggt
     2941 tgaaagtttt cttttcatat aatcaccaac ttgtatcaga tttagtttac ttaattagag
     3001 tataacagtg ttgtaacttc aaagagtatc taaaagatta caaatgttag ataaaatcat
     3061 caataatgta atttataaca cgtagctata aactatgcaa taatataaat ggaggtataa
     3121 gtaaatgaaa aagttccatg ttttgctaac agtttaaata aatattaggt agaaaagaat
     3181 gtatatagga atttgtggta aaattatcaa tttaaaagta tgatttagtg tgatacagtt
     3241 cttataggta atggataaca aatagataaa ggaacagttt ctttcttaga acatattttg
     3301 ttatgacttt aaacaaagtt aatattaatg tcaataatat agtaaaagaa taaaaatcat
     3361 atagtataat aaaaaagagt gtttctaaac actctttttt aatcattttc atcttctaca
     3421 tattcaagta tatcacctgg ctgacagtct aaagctttgc aaatttcgtt tagagtggta
     3481 aatctaatag cttttgcttt tccagttttt aaaattgata aatttgcatt tgtaatatct
     3541 attttttctg ccaaatcttt aagtgatatt ttcctttttg ccatcattac atctaggtta
     3601 actactattg ccataacatc acctctatat tgttaaatca ttatcttctt ttatttttat
     3661 agctttatca aatgtttctg caattacaaa gcacattagt gcaaatataa aagaaataac
     3721 cataagtggt gagataccgc cactattatc cccaaatatc attataagac tagtatttga
     3781 gttataacca attacacatt caattatagt gtttatcaat aaacaacatc ccattacttt
     3841 aaaccttttt acattttcaa gtacgaatgg ggaatactta gtactattaa ttataaatat
     3901 caagaataag atgactataa agtagtcaga aaaatatagt attcttgcaa ttaaagcgat
     3961 tactttgtca gaactaaaat catataaaaa cataggtatc atacataaga atattatacc
     4021 agcaactcct aaaagtgata ataggaaata tagaaatact gataatatat tactaaaaaa
     4081 ttttgtaatc ataaaatatc a
//
LOCUS       DAELJO010000269         4075 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELJO010000269 DAELJO010000000
VERSION     DAELJO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.271"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(89..1186)
                     /gene="ychF"
                     /locus_tag="KPB88_003622"
     CDS             complement(89..1186)
                     /gene="ychF"
                     /locus_tag="KPB88_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG0055856.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1502..2767
                     /locus_tag="KPB88_003623"
     CDS             1502..2767
                     /locus_tag="KPB88_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0055857.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2782..4068
                     /locus_tag="KPB88_003624"
     CDS             2782..4068
                     /locus_tag="KPB88_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0055858.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
BASE COUNT         1564 a          459 c          650 g         1402 t
ORIGIN      
        1 ataattatta taagataata ttgtattatt ataatcttag ttttttagtt attatatata
       61 atagtcaaag tgtatacgat taatcatttt aaacattaaa tctaaataat attacatctc
      121 catctttaac tatgtattct ttaccttcta atttaaccaa ccctttttct ttagcagcag
      181 ccatagtacc atgctcaacc aaatcattaa aggctattgt ttctgctctg ataaatcctc
      241 tttctatatc agaatgtatt tttcctcctg cctgaggagc tttacttcca acttttatag
      301 tccatgctct aacttctttt ggaccagcag ttaagaatga aattaaacca agtaaagcat
      361 aagaagattt tataagctta tcaagccctg attgttcaag acctaatgtt tctaaaaact
      421 ctttcttttc ttcagcagaa tcaagttcag aaatttctgc ttctatttgt gcacatacta
      481 caacaacttc ggcatcttct gtttctgcaa atgctttaac ttgttgaaca tatttattat
      541 tttctccatt atcagctaaa tcatcttctg atacatttga agcatatatt acaggcttag
      601 atgtaagtaa atccaatgaa tttacgaatg tttgttcatc ttctgtaaat tccatagttc
      661 ttacacactt actttcttct aaagtagaca ttattgattt taataaatca agttcagaac
      721 ctaaagtttt atcagcctta gcagcttttt gagttttatt aattcttctt tcaagaatct
      781 ctatatctga gaaaattaat tctaagttta tagtttctat atcacgaagt gggtctacag
      841 aaccatctac atgtacaaca ttttcatcct caaaacatct aacaacatga actattgcat
      901 caacttctct tatatgtgat aagaatttat ttcctaaacc ttctccttta gaagctcctc
      961 taactaaacc tgctatatca caaaattcta tagcagtagg aactattttt tcagaattat
     1021 ataattcttg tagtttgttt aatctttcat ctggaacaga tacaactccc acatttgggt
     1081 ctattgtaca aaaagggtaa tttgcagatt ctgcacctgc ttgagttatt gcgttaaata
     1141 gtgtgctttt acctacattt ggtaaaccaa ctatacctaa tttcatatta atcgtcctct
     1201 ctcttattct tttttatatt ctttatataa ctttaaacaa tcaatctata ggattgcaaa
     1261 attgttttaa ttatagacat acatattgat tatacatcat atatatcaag cttgtaaact
     1321 agactttaaa gcatattgat ttaaataata tttgtattta atttaaataa aaatattgct
     1381 tttagaaaat aataaaaagg atataattaa atagaaggtt tttatatgtg agtatatgta
     1441 attaaaaaat aaataaattt caattttaaa attgatgttt aaataaaaat aggaggtaca
     1501 tatggttaat actaaaaaaa taataaattt aggtaataat attaatctta ctttgataaa
     1561 aacagaaaag tttaaatcga atcttataag tttatatgtt cagagactat tggatgaaaa
     1621 agaaactaca aaaaatgctc taataccatc aataatagca agtggaagcg ctaaatatcc
     1681 tagtgctaga gcaatatcca ataaattaga tgatttatat ggttcatcta tgggagctga
     1741 tgctgtaaaa cgtggagaac gtcaagtatt gagttttaaa gtaattaaca taagtgaaaa
     1801 gtatttagat gagagtatat ttgaagaagt agtagaattt tttaatgaag taataaataa
     1861 tacgcttgtt gtagatggtg ggtttaaaga agaatattta aatattgaaa aagagaattt
     1921 aagagaaaag atacaatcaa taataaatga taaaaaggaa tatgcgcaag ataaatgtat
     1981 tgaggctatg tgtaaaggag agagatatag tgtaagtgaa tttggttatg aagatgaaat
     2041 agatagtata acttcaagag agttatatga acattataaa aacatactta aaacatcacc
     2101 tatagatata gttgtagaag gtaattttga tgaagataaa gttgtagata taataagtaa
     2161 aaatctaaaa tttgaaagag aagaaattat aaatatacca agagcagatt ttataaaaaa
     2221 tgtggatgag gtaaaagtta tagatgaaca aatggaaata actcaaggta agttagttat
     2281 gggttataga gcaaatgtgg attatgcaga catagataaa tattatgctt tagtagttgg
     2341 gagcaatgtc ttaggtggtg gtccacactc taaattattt gttaatgtaa gagaaaaaga
     2401 aagtttatgt tactatatat tctcttcaat agagaagtat aaaagtatca tgtttatatc
     2461 atctggtata gaaactaaag attatgataa agctgtagaa ttgataaaaa aacaagtaga
     2521 aagtgtaaaa gcaggtgata tatctgatga agaattagaa aacagtaaac ttgctttagt
     2581 taattctatg aaatctataa ctgataatat aggtggtatg tctgattttg ttttctctca
     2641 atctatggca aagacaaatt ctgaagtaca agacataata tctagtatag aaaaggttac
     2701 aaaaaaagat atagttgaag ctataaaaaa tatagagctt gatactgtat acttcttgag
     2761 aaattaatgg aggagatgtc tatggaaaaa atagttaatg atatattgaa agaagaagtt
     2821 tattatgaga aattgcaaaa tggattagat gtatatttta tgcctaaaag gggttttatg
     2881 aaaaagtatg ctattttggc tactaattat ggttcaaatg acttagaatt tgtaccaatt
     2941 ggagaagata agaaaattag agtgaatgaa ggcattgcac attttctaga gcataagatg
     3001 tttgagcaac ctgatggagg agatgcattt gataaatttt caaaattagg agtaaatgca
     3061 aatgcattta ctaattttac aatgacagcc tacttatttt ctgctactga aaatttttat
     3121 gagagtttag aacatctaat tgactatgtt caaacacctt attttacaga tgaaaatgtt
     3181 gaaaaggaaa aaggtataat agcacaagag ataaaaatgt acaatgatga ccctgattgg
     3241 aatgtatact ttaattgctt gaaggcaatg tatgtaaatt atcctgctag aattgatata
     3301 gcaggtactg ttgatagtat atataagata actaaggaag agctatataa atgttataat
     3361 actttctata atccaggaaa tatggcttta tttgttgttg gtgacttaga tgtagaaaaa
     3421 gttatagatg taactaaaaa gtcaaacaat tacaaagtag ataaactctc taagagtata
     3481 gaaagatttt atccagaaga accagaaggt gtaaaagaaa aagaggttat agaaaaattc
     3541 cctatatcta tgcctatgtt caacataggt tttaaagata gtaatgttgg tttaaaaggg
     3601 aaagaacttt tacgtaaaga gattgtaact gatatacttg tagggatgtt atttaaaaaa
     3661 ggaagtaagc tttatgaaga tttgtatatg caaggtttaa taaatgaaaa ttttggagct
     3721 ggattttcgt ctcaagtgga ttatgctttt agcataatag ctggagattc taaagagcct
     3781 aaaaaagtaa aagaaataat tcttgattat atagaaaaat caaaaaaaga aggattatca
     3841 aaagaagagt ttgagagaac taaaaagaaa aaaatagggt catttattaa gtgctttgat
     3901 tctataaact ttataggtaa tagttttata tcttatgttt ttaaggatat aaatctattg
     3961 gagtatttag atgttattaa agatattact tttgaggaag ttgaagaaag attaaaagag
     4021 cattttaaag aagagtattg tgttatttct attgtagagc ctaaatagaa actta
//
LOCUS       DAELJO010000270         4051 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELJO010000270 DAELJO010000000
VERSION     DAELJO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.272"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..891
                     /locus_tag="KPB88_003625"
     CDS             <1..891
                     /locus_tag="KPB88_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0055859.1"
                     /translation="RYDTNKKIINKFYNGAKEFYIASGTDLVYALVGSTIAKNTPIVL
                     VDDNSNKTALKGATKITAIGNLNNNAIEQSLNITKNIASPTTGGKYTVKEATERIAYI
                     LDEKVFVDYESAIKAKTDPDECFRYYIKNFTYQGKDYYAIGADDDDFRFLVNMNNLND
                     ILQWYSNDTIKRFDAEGYLKERKAIDMAFEACSSKYGISEDDLILDREEHNISTDFYV
                     VVLYESSGIDSYWVFIDTDTWTVHDIQRLNQNEELVSYHDVNPNTEADKHYNRDVDDV
                     NIIFVPSENNPFDNDVDGIN"
     repeat_region   1137..1627
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1137..1165
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            complement(1661..1885)
                     /locus_tag="KPB88_003626"
     CDS             complement(1661..1885)
                     /locus_tag="KPB88_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055860.1"
                     /translation="MLRQLRKMRHLTQLELAEKINYHKSYISKLEKGDYKNVTVTTIV
                     DLAIGLEVNYLIVAAVFIMEELKRRKEKID"
     gene            complement(2447..2818)
                     /locus_tag="KPB88_003627"
     CDS             complement(2447..2818)
                     /locus_tag="KPB88_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055861.1"
                     /translation="MNVSQRIKYLRENMNMSQKELSEKANINTSVMNRIESGERAIRD
                     EELIIFAKIFDVSTDYILGLSDTEKLNIDETYEFIDSLNSPDDIKELIKIAISLDDET
                     RDKMLKIARVFVTEKDKHNDN"
     gene            2984..3187
                     /locus_tag="KPB88_003628"
     CDS             2984..3187
                     /locus_tag="KPB88_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055862.1"
                     /translation="MKKINHDFIRKRRNTLNLSLQNVAKELGFKNASTYFKYETGQYS
                     IRADMLPKLSKILDCDIENFFTN"
     gene            3225..4016
                     /locus_tag="KPB88_003629"
     CDS             3225..4016
                     /locus_tag="KPB88_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG0055863.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRFQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
BASE COUNT         1572 a          417 c          635 g         1427 t
ORIGIN      
        1 agatacgata ctaataagaa gataataaat aagttctata atggagccaa agaattttat
       61 atagctagtg gaacagattt agtgtatgct cttgttggct ctacaatagc taagaatact
      121 ccaattgtgt tagttgatga caatagtaat aagactgcgt taaaaggtgc aactaaaata
      181 acagcaatag gaaatctaaa taacaatgct atagaacaat ctctaaacat aacaaaaaat
      241 attgccagtc caactactgg agggaagtat actgtaaagg aagcaactga aagaatagct
      301 tatattttag atgaaaaagt atttgtagat tatgaatcag ctattaaagc aaaaacagac
      361 ccagatgaat gttttaggta ttatataaaa aactttacct atcaagggaa agattattat
      421 gcaattggtg cagatgatga tgattttaga tttttagtaa atatgaataa tttgaatgat
      481 atactacaat ggtattcaaa tgatacaata aaaaggtttg atgctgaagg atatttaaaa
      541 gagagaaaag caatcgatat ggcatttgag gcatgctcta gtaaatatgg tattagtgaa
      601 gatgatttga tactagatag agaagaacat aatataagca ctgattttta tgtggttgta
      661 ttgtatgaga gttctggaat agattcatat tgggttttta tagatactga tacatggact
      721 gttcatgata tacagcgttt aaatcaaaat gaagaattag tatcatatca tgatgtaaac
      781 ccaaatacag aagcagataa gcattataat agagatgttg atgatgttaa tattatattt
      841 gttccatcag aaaataatcc ttttgataat gatgtagatg gaataaatta aatatttatt
      901 ataaggagtt aaactataaa ttgatataaa tatttaaaaa taatgtattt atgatataat
      961 aaaactatgg aaattttgca gtgagcgata tttatgataa aatggggctt agcagttgaa
     1021 atataaggca ttgagggtgt atgataagtg ttatcatttg cactactgcc cgctcactgc
     1081 aaatttaaga gagttgtata tgcgtaggca ttgaaaatac tcaacttatt ttggaggttt
     1141 tatattaact atgtggtatg taaattttag atagtaacta tatgatgtaa tttctttttt
     1201 tgttttatat taactatgtg gtatgtaaat ttgtattacc atgtttagca aagaattgat
     1261 tatactagtt ttatattaac tatgtggtat gtaaattttg ttgttttcat atctactgat
     1321 tgaaatatct gaaagtttta tattaactat gtggtatgta aattcataat tgacagcgtc
     1381 accatctatt agcccaaaca gttttatatt aactatgtgg tatgtaaatt agatgaggag
     1441 aacaggaaag caatagagaa aattagtttt atattaacta tgtggtatgt aaatataaat
     1501 atctctgcaa cttgcatatt ttatttttat tgttttatat taactatgtg gtatgtaaat
     1561 tcttgataat gtacatttat tttaatactt ttctcttgtt ttatattaac tatgtggtat
     1621 gtaaataatt tttaaataat agaaatagac tagtatttag ctagtctatt ttttcttttc
     1681 ttctttttaa ttcttccatt atgaatactg ctgcgactat taaatagtta acttctaaac
     1741 caattgctag gtcaactatt gttgtaacag taacattttt ataatcgccc ttttctaact
     1801 tggaaatata acttttgtga tagttaattt tttctgcgag ttctaattgt gttaaatgtc
     1861 tcatttttct cagttgtctt aacataaatt taaatcacct tttaatttat tttttagtaa
     1921 attcagataa ttatattatg tgcatttttg tggggaaaag tttcctatta gtcgattttt
     1981 ttgttggaaa tttgtgctaa aatataagta agaaataact ctagctagat agagaaaaaa
     2041 tagaaaattg tagattttga taagtcttgc aattaaattt atgcaaatgt aagcactttt
     2101 taaaagatag gggatggttg taattaattt tgcgaacata tgctcgtgat ttagaaatac
     2161 ataagactta taaacaaata catagtatat ggggatggtg tgtatggaat attcaagaag
     2221 attaatatta gaactagaag atttgtttaa aaaatttaaa gaagctgaag aagatgaatt
     2281 taaaaaatat gaaaaaatgt taaaagaaga aaaagaaaaa agagaggatt agttcctctc
     2341 ttctaaacat atgtaatttg ttatgtaacg gttatattat ttatatttca tgcaatatct
     2401 tcatcaaaaa aatgttatca acatttataa taccattatt tcagttttaa ttgtcattat
     2461 gtttgtcttt ttctgttaca aatactcttg ctatttttag cattttatct ctagtttcat
     2521 catctaagct aatagctatt tttattagtt cctttatatc atcaggacta tttaaactat
     2581 ctataaattc ataagtttca tcaatattta gtttttctgt atctgataaa ccaagaatat
     2641 agtcggtaga tacatcaaaa attttagcga agattattaa ctcctcatct cttattgctc
     2701 tttcaccaga ttctattcta ttcataacac tcgtatttat attcgctttt tcagacaatt
     2761 ctttttgtga catattcata ttttctctaa gatatttaat tctttgactt acattcattt
     2821 ataatcacct cggaaatttt ctattatgga aatataatag catatttttc tataatagaa
     2881 ataaaaaatt tctaaaataa atacaaaaaa gtcttgaatt tctgttttag aaatggtatt
     2941 attgaaatat ggaaattgct aaaatagaaa ttgaggtgaa tgtatgaaaa agataaacca
     3001 tgattttata agaaaaagaa gaaatacatt aaatttatct ttacaaaatg ttgcaaaaga
     3061 attaggcttt aaaaatgcat caacttattt taaatacgag acaggacaat attcaattag
     3121 agctgatatg ttacctaaat tatctaagat actcgattgt gatatagaaa atttttttac
     3181 aaattaaatt tctaaaatag aaattttata aggagggtta aaaaatgaat aacttacaag
     3241 taatagaaag aaacaacgaa agagttttaa ctacacagca attagcagat gtatatgaaa
     3301 cagatgcaag aaatataagc aacaatttta ataataacaa agatagattt attgaaggta
     3361 aacattattt tttattgcaa ggcgatgatt taaagaattt taaaggtatt catacagaat
     3421 atgaaaacct aaaatttact tcaaaaatgt atctttggac ggaaagagga gcaaacagac
     3481 actgcaaaat attagataca gataaggctt gggaacagtt tgataactta gaagaaacat
     3541 atttcaaagt taagcaacat aagccaactt gtatagaaga tgtattaata gaaagtttaa
     3601 aagaaatgaa agatttaaga tttcaagtta atcaagcaaa tagcattgct ttagaagcaa
     3661 agacagaggt tgaaacaata aaagatgtag tttcattaga ctcaaatagt tggagaacaa
     3721 atacacatca actaattgca agaatagcaa aaaaacaagg tggttttgaa catataaata
     3781 tgcttagaac agaaagttat gaattattaa ataagagatt tggagttgac ctacatagaa
     3841 gattgatcaa taaaagaaga aaaatggcag aagaaggtgt atctgaatct aaaagagaga
     3901 aagttaacaa tttagatgta atacaagatg ataagaaact catagagggg tatgtagcta
     3961 ttgtaaaaga tatggcttta aaatatggaa tatcaagtga tttaagtaag aattaggtta
     4021 aataaaacta gcactttgaa aactaaatac a
//
LOCUS       DAELJO010000271         4045 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELJO010000271 DAELJO010000000
VERSION     DAELJO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.273"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            91..1011
                     /locus_tag="KPB88_003630"
     CDS             91..1011
                     /locus_tag="KPB88_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG0055864.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1126..2283
                     /locus_tag="KPB88_003631"
     CDS             1126..2283
                     /locus_tag="KPB88_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0055865.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2368..3867)
                     /locus_tag="KPB88_003632"
     CDS             complement(2368..3867)
                     /locus_tag="KPB88_003632"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG0055866.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         1596 a          561 c          619 g         1269 t
ORIGIN      
        1 ttcaaattcc ttgacagtaa taagtaagta attgtataat caaactgcaa tatattaata
       61 atattagtga aaatgaaagg attataaaat atgtataaat cagaaaattt ttcaaaaaaa
      121 tctgtattaa taaattcatt tttaagtaaa tttttaaaaa taattggatg gggtttggta
      181 agcttattta cattatcagc cttagtactt tcaactattt ccattataat tggatgggct
      241 gtaatacttc ctattcaagt atgtgcagta tttttactta taggaggagt ttgtattttt
      301 aaaggtaaga gattaggaga ccaaatttct cgttataaaa aatattgtac aattataaac
      361 aatagaaata ttattccagt agaacttatt gcagaaaaca catctaaatc attaaatttt
      421 ataataaaag atgtacaaaa gatgatagac aaaaactact ttataaatac ttatattgat
      481 aaaagaaata atcagattgt gttaacaaat gaagattttg ttccaccaag atatgaagat
      541 gtagcatatg aagtcaaaga agaagaaaag actgatgaat ccaatgaagt tgatacaatt
      601 ataaataaag gaatgaatta tctaacacaa ataaaagaag ctaataaaaa tattaaatca
      661 gaatcaatgg gcgataaaat tgtacaagta gaagatgtta caagtaaaat atttgatgtt
      721 gtaaaacatg acccatcaaa acttactcaa atacaaaaat ttatggatta ttatcttcca
      781 accactttaa aactattaaa ttcatatcat acattggaag aacaaggaat tgatagagaa
      841 aatataacaa caactatgga aagtattgaa aatacaatga atacgattgt agtagctttt
      901 gaaaatcaat tggactatct gtttgaagat gaagccattg atatttctac agatattact
      961 gttttagaga atatgttagt ccaagaagga ttaacgagtg gtaaatttta acttcaatag
     1021 aaaaaatatt ctgttatttt taaacaatta attagtcata aagtataaaa tgtgtattat
     1081 ataagttaat cagtacttta tgaaggaaga ggagagaaag atacaatgaa taatttagat
     1141 gatataccag ttatgccaac tttgacactt gacccatttg gagaagaatc aaacactaat
     1201 gatatagata attctgactt attaatgaaa aaggatgaaa aagacccaga agaagaaaaa
     1261 ctgtctgaat ctgaacgcaa gatggtaaag gaatttgcgg aaaaaataga tattacaaat
     1321 acaaatatga ttttgcaata tggagctggt gctcaaaaaa aggtagcaag tttttctgaa
     1381 acagctttaa agagtgtaaa gacaagagat ttaggagaag ttggagatat gcttacaaat
     1441 ctagttactg atttaaaagc attttcagca gatgaaaaag aacaaagtgg ttttttagga
     1501 atttttaaaa aggcaaataa taaaatttca aatttaaaaa ctaagtatga tagtgcagag
     1561 gtaaatgtgg ataaagtaag caaagagctt caaaagcatc aggttaaaat attgaaagat
     1621 atagcaatgc ttgataagat gtatgaattg aacttagctt actcaaaaga gcttactatg
     1681 tatattatag caggaaaaca aaaattaaag gatatgaaag aatatgagat gccaaagttg
     1741 agagaaaaag ctcgtttatc tggttcaaca gaagatgctc aatctgtaaa tgatatggtt
     1801 tcactttgtg acagatttga aaagaagatt catgacttag aattaactag aatggtatcg
     1861 cttcaaatgg ctcctcagat aagattggtt caaaatagta ataatctgat ggcagaaaag
     1921 attcaatcaa ctatagtaaa taccattcca ttatggaaaa atcaaatagt gcttgctctt
     1981 ggaatttctc acttaaatca agctatgaaa gcacagcgtg aagttagtaa tatgacaaat
     2041 gagcttttat taaagaatgc agaaactttg aagatgggaa caatagaaac agcaaaagaa
     2101 tcagaaagag gaattgtaga tatagacaca attaagaaaa cgaatcaatc tctaatatct
     2161 actattgatg aggtagttaa aatacaacat gatggtagaa taaagagaca agaagcagaa
     2221 gttgaattat caaaaataga aaatgaactt aaaagtaagc ttcttgaatt tacagttaaa
     2281 taaaaattat actaatataa aaagagactg tctcaaaata gagataagtc tctttttcat
     2341 tgtttaaatg attaaagtat atttatatta taaatttcta ttatctttta acataacatc
     2401 atgagctcca ccttcaacta tactagtaga agatacaagt gtaagtttag catttttttg
     2461 aagttcagga atgctaagtg ctccacagtt acacatagta gaacgaactt tacttaaaga
     2521 aagagttaca ttgtctttca aagaaccagc ataaggcaca taagagtcaa caccttcttc
     2581 aaaagaaagt ttcttatctc caccaaggtc atatctttgc cagtttctag cacgagcaga
     2641 accttctccc caatactctt tcatataact accatttata ttaactttgt tagttggact
     2701 ctcatcaaaa cgagagaagt atctaccaag cattataaaa tctgcaccca tagctaaagc
     2761 taaagttata tgatggtcat aaacgatacc accatcagag caaattggaa tataaatacc
     2821 tgttttttca aagtattcgt ctctagcttg agcaacttca attatagaag tagcttgtcc
     2881 acgtcctata cctttttgtt cacgagttat acatatagaa ccaccaccaa taccaacttt
     2941 aacgaagtca gcaccagctt cagctaggta aaggaatcct tcgcggtcaa ctacatttcc
     3001 agctcctacc ttaactgtat caccatattt ttcacgaatg aaatcaagtg ttattttttg
     3061 ccactcagaa aaaccttctg aagaatctat acataaaaca tcagcaccag cttcaactaa
     3121 agcaggaaca cgttcagcaa agtctctagt attaatacca gctccaacta catatctctt
     3181 agaagaatct aaaagttcaa gtggattttc tttatgagaa gagtaatctt tacggaatac
     3241 catatacata agattaccat tatcatcaag tacaggaaga gaattaagtt tgtggtccca
     3301 tataatgttg tttgcttcct ttaaagtaac atctttgtta gcataaacta tagatgacat
     3361 aggagtcata aattcactaa ctttagtatc taaatccata cgacttatac gataatcacg
     3421 acttgcaaca atacccaata atttaccagt tgaagttcca tcttcagtaa cagctactgt
     3481 agaatgtcca gttttttctt ttaagtcaag tatgtctttt aatgtgttat caggtttaat
     3541 atttgaatca cttacaacaa aaccagcttt gtgggattta acacgtgcta ccatagcagc
     3601 ttcattttca atagtttgag aaccgtatat aaatgaaata ccaccttctt tagcaagtgc
     3661 aatagccatt ttgtcatcag aaacagattg cataatagca gaagtcaaag gtatattcat
     3721 ataaatatct gcttcctcgc cctttttaaa tttagtaaca ggtgttttta aacttacatt
     3781 agcaggtata caatcagtag aagagaaacc tggtacaaga aggtattcac tgaaagtatg
     3841 tgatggtgta tcaaaataaa acgccatttt ttaatcactc cttatatttt tatacttttt
     3901 ataattgata gtattaatta taacttattc tttaatttta ttaaagtatt ttgtcaaata
     3961 ttgagatttt tgaaaaattt aaactatttt aattaaattt gtttaatata tattgcctta
     4021 tagggtaaag ttgtatatta aataa
//
LOCUS       DAELJO010000272         4007 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.274, whole genome shotgun sequence.
ACCESSION   DAELJO010000272 DAELJO010000000
VERSION     DAELJO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.274"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(93..1514)
                     /locus_tag="KPB88_003633"
     CDS             complement(93..1514)
                     /locus_tag="KPB88_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0055867.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(1660..2430)
                     /locus_tag="KPB88_003634"
     CDS             complement(1660..2430)
                     /locus_tag="KPB88_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055868.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(2435..3637)
                     /locus_tag="KPB88_003635"
     CDS             complement(2435..3637)
                     /locus_tag="KPB88_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0055869.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         1343 a          722 c          408 g         1534 t
ORIGIN      
        1 caataaaaaa gagacttatc tctattttga gatagtctct tctttgaaca taatttaaat
       61 ataatttttt aatttttata gttattattt tactaacttg ccaatttatg attattttgt
      121 tctttaatat gttttgcttt tttaaagaat aaaagaccaa gaattattgc tacaataaca
      181 cctattccat tagatatatt aactggtagt ctaaatcctt cttttgcttg cattatataa
      241 ctaaatgtta ctacagtcat aaaaagtgct ggtaatgtag ttatataatg gtttttattt
      301 gctttgacta aatatgcaga accagtccac aacattatca ttgcaagtgt ttgatttgcc
      361 caagagaaat atctccaaat tatattaaaa tctataaatg taagagcaac accaactgcg
      421 aataatggta aagctattaa aaatctattt ttgactgcat cttgattata attcattgca
      481 tctgctattg tcagcctagc acttctaaat gctgtatctc cagaagttat aggacatgcc
      541 acaactccaa gtatagctaa agctcctcca ataggaccca ttaatccaac agatactgta
      601 tttactactg ttgctgctcc accatcatta aatatcacat taagttgagg tatgccatgg
      661 aaatatgaca tggctgctgc tgcccaaact aaagctatta taccttctgc aaccattgac
      721 ccataaaata ctggtcttgc ctcactctct ttctcaacac atctcgccat aagtggtgat
      781 tgagttgcat gaaaacctga tattgctcca catgctattg ttataaataa gtatgggaat
      841 aaaggtgttc catttggatt catattttgt aaatttatat ttggtatgtt ataccccttt
      901 attattaatc ctccagctac acctactgcc ataattaata atgctgctcc aaataaagga
      961 tatattttac caatcaattt atctattggt aaaacagtag ccataatata ataaagtatt
     1021 atcactccaa cccatatttg tgcgtttaca cctgtcaatg tctttaataa tcctgctgga
     1081 cctgttacaa atacaactcc aaccaatact aaaagaataa cactaaatat tctcattgct
     1141 atttttgcac catttcctag ataaaggcct accaactctg atgcagatgt acctttgttt
     1201 ttcattgata gatatcctga agcaaaatcg tgtactccac cagcaaagat tgttccaaac
     1261 acaatccata aaaatgctga tggtccaaat aatgctcctt gtatagctcc aaatatcgga
     1321 cctaaccctg ctatatttag aaactgaatt aaaaagattc tcttccattt cattggcaca
     1381 taatctactc catccctaca agctatagct ggagtttgat ttttgtcatt ggttccaaaa
     1441 accttttcta caaaagtacc atagacaaaa taacctacta taagtactac tattgaacct
     1501 aaaaagctta ccataaattc atccccttag tttttatata attgctttat aaatttttat
     1561 gagtttaaga aaactatttc cagttcaaat agtaatactt ttttatgcta atataaatat
     1621 taattacatt aagtgcatat ttaagtacat gaaatgactt tatatatgca ttaacagcct
     1681 aaattctttt actttgcttc tacttacagt tacttcataa tcactatttt ttaattttaa
     1741 tatgtatgta ttattaaacc atggtataat ttcttttact ttggttaagt ttacgatata
     1801 agacctatgt gttctaaaaa atgttttatc atttataaga ttttcgactt ctgatatgcc
     1861 ttcttttaat ataaactttt ctttctcagt atatataaat gtctgccttt cattagcttc
     1921 acagtaatat atatcatcaa tatctattac aactaattta tcacctttcc aaaggctaat
     1981 tttatgtgtt atttcttcta tctcctgatt tactgcttcc ttcttatatt tgtataaatt
     2041 actatttaca ttagatagtt caatatcact catctcactt ttttctaatt tattcagcat
     2101 agaaataatt ctctcttcag aatatggttt caatatataa tcaaatacat ccaaagaaaa
     2161 agcatctact gcataacttt catatgctgt tataaaaaca attttaggtc ttgatttaaa
     2221 ctgatttaat gtttttgcaa gtagcattcc atcaagatga ggtatattta tatctaaaaa
     2281 aattacatct attttatttt cctgaataaa gtctaacacc tctataccat tggtaaattc
     2341 acttactacc tctattccac ttttattttc aataaaatat ctcaattcct ttcttgctgg
     2401 aaattcatct tccactatta tagctctcat acttctactc ctttacataa aatactatca
     2461 aagtcccatt atctattctt cttatattaa gccctcttcc atacatcagt ttaagcctta
     2521 aatgtacatt ataaagccca atcttatttt ctggcatatt atcttgatat acattattta
     2581 taatttcctc acttatacct ataccattat cctctattga tattctcaca gtattttcta
     2641 atgaatacgg taactttttt atggatattt tcactacacc agctctacca ctttctaaaa
     2701 taccatgtat tattgcattt tccactaatg gttgtataat caaacttgga atctttatat
     2761 ttacatcttc tatatcataa gatacagtta gtaattctcc aaacctagct ttttctatct
     2821 ctacaaaaga tttaacttgt tcaatctctt tatttatatc aattaagtta tcactatatt
     2881 caagattata tcttagataa gttgataaat ttattattaa ctctctagcc ttatctggac
     2941 taaatcttat aaatgaagct attgtgttta gggaattaaa caaaaaatgt ggattaattt
     3001 gtctttgaag tgcttttatt tctgctttat ttctcgcttc tatatctttt ttatctttct
     3061 caatcccctc tactatagat ataacaaatc ctattccaat ctgcccaact attaaaggta
     3121 aatatattcc accgattatt tgaattgcta aagaatgtgg atatgatatt aagtatataa
     3181 gtaatatagt aaaactttca gaaatcatgc ctaccaatat tccatacatt actctatgtt
     3241 gttttggtat cctcttatat agaaatcctg ataatacacc tgctagtata cttgataaaa
     3301 gacatggaac agatgttatc cccccaatat ccataaaata tctatgaatc cctgaaaaaa
     3361 ctcctgctgt aatacctacc atcggaccaa ataaaatgcc acctgatact atagatatta
     3421 ttctagtgtt tactattgac cccatataat ttataccact ataagttcca aatatggcta
     3481 aaaaagtaaa aactaatgct atacagacca agtcattaaa actatattct tccttttgaa
     3541 atatctgctt aaatattttt aactttgtta ttagaagaaa caatactaca ataaggcttg
     3601 tcttttctat taatgcgatt aaaattgatg ttttcataat ccccctttaa aaaataaatt
     3661 tttacacact ctaaatttat tatggttgtt ataaattaca tatttgaagt aatcctatca
     3721 caaaactttc cacatttaat cttttaatta aataagaaat agttgtactt ctattaacaa
     3781 ctaatactag ctagcataag cttgtaataa cttgtagtta ttattaactt tacaattcta
     3841 gtatttaatt ttatacttta gaaatctctt ttcttagaaa taatactttc gaatatcata
     3901 taatacaata ttatgtagat tcttactcat acaacatatt tctttaaaaa ttttatacta
     3961 ttctttgttc aaatatctca tttgatttaa ttgtgttaaa cacacat
//
LOCUS       DAELJO010000273         3994 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELJO010000273 DAELJO010000000
VERSION     DAELJO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.275"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..690)
                     /locus_tag="KPB88_003636"
     CDS             complement(<1..690)
                     /locus_tag="KPB88_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055870.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPF"
     gene            complement(691..1566)
                     /locus_tag="KPB88_003637"
     CDS             complement(691..1566)
                     /locus_tag="KPB88_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055871.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1570..1938)
                     /locus_tag="KPB88_003638"
     CDS             complement(1570..1938)
                     /locus_tag="KPB88_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0055872.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2120..2779)
                     /gene="deoC"
                     /locus_tag="KPB88_003639"
     CDS             complement(2120..2779)
                     /gene="deoC"
                     /locus_tag="KPB88_003639"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG0055873.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2815..>3994)
                     /gene="murJ"
                     /locus_tag="KPB88_003640"
     CDS             complement(2815..>3994)
                     /gene="murJ"
                     /locus_tag="KPB88_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0055874.1"
                     /translation="EPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSIPYN
                     IVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSLIKL
                     LALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIVSVM
                     YPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAFDAR
                     ATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLVLIK
                     PMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVLSYF
                     TYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLHLKK
                     "
BASE COUNT         1342 a          676 c          398 g         1578 t
ORIGIN      
        1 aaaaggattc tgaaatgaat aaataatttt ttctaaatac aatagcattt tatgcatatt
       61 tttagttgca aaattaacta tataaattat cattaatatc ggtaatccta tcaatatagt
      121 aatactagta acttttctaa ctctatatgt aaaagctcca agtaacactc ctataaaacc
      181 tatacatatt tcatttatac atatttgtac aaaataattt aaataagtta gtggcatact
      241 tgatgttgaa ggatctatca gtgctgatat agatgtccaa atcatatctc tctttagtaa
      301 aactgatagt tctatactgt aacctgttat aatttctata aataacttcg ataaaaaaat
      361 caaaactata cctaaaatag aaatcacaaa gcttaaaata attaaatcta tgacaatagc
      421 ttttatacaa ctttttctat ctgctcttat acttaaagct cctgaaaagt ctttatttgc
      481 tatatttact ccatacacaa atagcagtat agttatatat gttaaaatag gattcacaat
      541 cgtggataaa ttagttttag atgcttctcc aaaaatcttg aggataaaca ctggcaaaat
      601 attcataaca attcccacaa tcataaatat aattatatac aatttagtat ttttattaaa
      661 aaattttata tatggcttta attcattcaa ttacaattcc tccttcttag tcatgttaac
      721 aaacatatct tgtagcccta tgtatcctac atcaatattt gaatttttaa gtattttttc
      781 atcattttca ctaaaatcac catagtaaaa ataagcgact gcatttccaa atgccttttt
      841 aggttttata ttttttatac attctaattt ttctaactct tctttatttc cagtaatata
      901 atgagctttt tgttttatga catcaatttc ttcatctata ataatttttc cctcatttaa
      961 tattattaca tgctccagta aatcatctat ttcatcaatc aaatgagttg atattattat
     1021 tgtccttgga ttttttatat aactatctaa aattatgtta taaaactctt gtctatttac
     1081 tgcatccaaa ccaattgttg gctcatcaaa tatggtgata gctgaattag aacaaatacc
     1141 tattatattt gaaagtatag ttttcatacc tcttgacagt tgtttatact tttttttagt
     1201 atttaaatca aaatatttgc ataattcttc ttctaagcct ttatcataac ttttataaaa
     1261 actagaagaa aattcaaata tctgacctac tttaaaatct ggactataaa attctttttc
     1321 cctgacgata caaacttctt ccaaaacttt tatatcttct tttgggttct ttcctaatat
     1381 ttctatctca ccttcatttt gaattaactg atttaccatt atatttaata atgttgtttt
     1441 tccagctcca ttcttaccta ataaaccata tatcttattt ttttcaaagt ttagagacat
     1501 attattaaaa atattttgct tcttaaaact gtgagataaa ttttctatct taatagaact
     1561 actcatatat taatcctcct tgaaattaat tataatatca accaattctt ctctatttat
     1621 ttctaatttg ttagcttctt gaagtaggtt ttttacaaaa ttatgtttaa aattttcttt
     1681 tctagcttct tttataatag tttttgcacc atctgataca aacatcccta tccccctctt
     1741 cttatataaa atatttttat caaccaatat atttatacct tttaatactg tagctggatt
     1801 aattttataa agctttgaaa gctctgttgt tgatggaacc tgttcttctt cttttattcc
     1861 atttgatagt atctcatctt caattgctcg tgctatctga ataaatatag gttcttcatt
     1921 atttaatact aatttcatag gtgcctcctt tctccattag ttagttactc aactaattaa
     1981 ctatataact tataatatct tgaaaaatat tttctgtcaa tactaaataa aaaacagagg
     2041 gaaaattccc tctgtttgtt gttctttata tttataatta aattttaatt tttgtaatcc
     2101 tatccataag ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata
     2161 ttgagctagt tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg
     2221 atttaactcc ccctgcagct tttattttta cattttttcc aacatgctct ttcataagct
     2281 ttatatcttc taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg
     2341 aacctgacat tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg
     2401 tctctataat gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt
     2461 ttcctatatt atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt
     2521 catctgctcc attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat
     2581 atcccaaagg gaaccctatc actgtacata tctttatttt accttttaga tattctgatg
     2641 ctcttttcac atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat
     2701 cacataaaat tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg
     2761 tttttaatat atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt
     2821 ttttaaatgc aattttcttt ttgcaatatt cagtatcata tcaacctctt caactttaaa
     2881 aatagtcatt agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac
     2941 aaactcatta aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga
     3001 taaaacaccc ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc
     3061 ttgtccaaaa tatccaactt ttctttttag gttcaagaag agtaacaata tacatgcaat
     3121 tgatgatgaa ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa
     3181 gtccaatact atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc
     3241 ttgtaatgaa taaaaaacct ttcccaaaat atctcttaat ccaaatgcag tcataccaac
     3301 tgcataaaaa attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct
     3361 ctcaaaaatg attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat
     3421 catagaaatt attatcatat ttatactaga tttaactgaa ctagtaaact tcttttgatt
     3481 attttctgca cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa
     3541 tgttcctgta acaaaaccat ttaatctatc tgcataagta agtgctggaa tgcttccttt
     3601 aactaatgtt gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc
     3661 tataaatact ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa
     3721 gtagtataag tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac
     3781 taccatagca accacagcac caattggaag tatgtatggt ccaaatatgg tacttaacat
     3841 tattgaaata attattacta tattataggg tatacttcca aatccaacta caataaagtt
     3901 ctctttaatt tgtaaaaatg ctgacataac acttgtaata cctatgaata ttattcctgt
     3961 tctgaacctc ccatgactaa agtcacaggg ttcc
//
LOCUS       DAELJO010000274         3993 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.276, whole genome shotgun sequence.
ACCESSION   DAELJO010000274 DAELJO010000000
VERSION     DAELJO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.276"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            189..1694
                     /locus_tag="KPB88_003641"
     CDS             189..1694
                     /locus_tag="KPB88_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0055875.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEIIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1808..2506
                     /locus_tag="KPB88_003642"
     CDS             1808..2506
                     /locus_tag="KPB88_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0055876.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2791..3879
                     /locus_tag="KPB88_003643"
     CDS             2791..3879
                     /locus_tag="KPB88_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055877.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1585 a          392 c          641 g         1375 t
ORIGIN      
        1 aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa aaaaataatt
       61 aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat atgaaagagt
      121 atgtacacat tttttttact aatttttcac aatatgaaca taaataattt acaggaaagg
      181 aatcttttat gaaaattcaa aatactgagt ggggatatat tgaatggaaa catacttatg
      241 atgaaaataa tccaaaacaa gctatgaata tatacattgc agtaactatg cctggtaaga
      301 aacattttaa tcatgttcat tatggtcaag agcagatgat atatatttta gagggagagg
      361 gtttgtatat tataaatgga gtttggaagc cattttatca aggtatgatt ttttatatag
      421 aatctggtag cacacatgaa actataaata caggcgatag agaaataaaa gaacttattg
      481 tttcaaataa tgtagatgat gtaggagaga gtgaaattat agatattaat cctaataatt
      541 atttaaagaa gaccttgata aattatagtg aaagtacatt aaatttgtat gcagcagtag
      601 aatcaattcg tggtcaattt atagacccgt ttaaaattcc attaattata tatgatgatt
      661 catggaatat agttttaaaa aatccttatt ttccactttt ttgttttgaa aaatgtaatc
      721 ctatgaaatt tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat caatttgtat
      781 gtgaatatgg gataactata tataatatac caatactgta caaaagcaat agcataggtg
      841 ttataagagg agggtatgta ctattatctg atttgaattt agatacagag cataataatt
      901 tatatgatat tccagaggga gctgctagaa gtataaaacg cctcttgaaa caaatatcta
      961 aaaatataat aaatttttgc tcttttaatg atataagaaa ggatttacaa gaaaaagaga
     1021 agactatagc aagaacatat cattatggag agcaacttga aatgaattta aaagtagctc
     1081 aagatatggt tactaattta agaataaatc atcacttttt atttaataca ttaaattcaa
     1141 tggcaagcat agcactagat gatggtagtt atgatttgta tagtgcaata atagatttat
     1201 caagaatgtt tagatataca atgaggtctg atttaagatt tgtagaatta gaatctgaaa
     1261 tattatacat taaaaattat ttgaatttac agaaattacg ttatggagat gcacttaaag
     1321 taaaatatct tattccagag aagttatata acctttctgt gccttttaac tttattcaac
     1381 ctattgtaga gaatgcattt acacatggat ttagagatat tgacacagaa aagcgaattg
     1441 aaataattgc aagattagat tctcagtatg ccattataga gattcataat aatggaacta
     1501 tcttagatgg aaataatata gacaaaataa aggcaggtat tcgtagtaat aatggtcatg
     1561 gtttgtcttt aatatataca aaattcacat ctgcttatgg aaataatttt gatatggata
     1621 taaagtcgag tgataatgaa ggaacttaca taatgattaa aattcctatt gaaaattata
     1681 aggagtatgt atagaacttg tgaaaaatta ataagtttta taaatgttaa gaaatatatt
     1741 agatgttttt atgttgtttt atatatctta cttttttata ttattaagct aaaagggagg
     1801 tattctcatg agaagagcac taataataga tgatgaagaa gctgttctaa aaataataac
     1861 acattttata gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa catctggtga
     1921 tgaagcagta gataaaatca ttggtttaga gccagacata gtttttatag atattcaaat
     1981 gccaatatac aacgggttag aagtaataga aaaaacatct catttatcta aaaaaataaa
     2041 ctttgtagta ataactgcat ttaactattt tgaatatgct caaaaagctt tgagattaaa
     2101 tgtaaaagat atactcctta aaccattgga tatgaaagaa tttactaagt ctgtagaaaa
     2161 aattattgga tatcaatata ccaataatga tttattaaat gagatactgg aatatataaa
     2221 tctaaactat agcaatgact taaagttaaa tgattgtgct agattatttt taaccaatcc
     2281 atctaatata tctaggattt ttaaaagtaa tttaaataca acttttatat catatctaaa
     2341 tcatataaga atagaaaaat ctattgaatt acttgaaaat actacaaaaa gtataaatga
     2401 gattgcagaa atagttggat ataatagcct aaataacttt tataaaaact ttaagataga
     2461 aaaaggaatg actccaaaag tttataaact gaacactaaa aattgaaatt gataaataaa
     2521 aagtcaatat agataattgt ttgagtgtgt ctgaataagt atactttaat tatgataaaa
     2581 attaaagcgc tttaaacata tttaataata ttaaacctta taaaagctgt acctatttat
     2641 ataaaaaaat atatataaat aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa
     2701 tactgtttta aaaagacaga attttcattt aatttactga gagtttatat ttcttaaagg
     2761 aaggtaactt tataatggag gtaatttaat atgtcagaca aagtgaatat gaaaaatatt
     2821 atctgtattg caggcgctta tctagcaaca gcaattggtt ctgggtttgc tactggtcaa
     2881 gaaattctac aattttttgc ctcacaaggg tatatgggtt taattggagc attaatatca
     2941 atggtattgt tttcatttct tggtgctgaa gttataacta gaggtagaga attaaaactt
     3001 aaagaaccaa caagaattta cactttttat tgtgggaaga ttttaggaaa attttacgaa
     3061 tggtttagtc caatattttt atttgggatt cttatagtaa tgatttcagg tgcaggctca
     3121 actttaactg aatactatgg tataaatcct tacttaggaa gagtattgat ggctgttgct
     3181 actttaatta cagtgtctat gggtttagaa aaattagtaa gcatacttgg acgtattgga
     3241 ccagtgatag cagtatttac aattgctgtt ggattaataa gcttgtttaa gcattttgac
     3301 ggtttatcta tggttggaga agctatgaaa aatattgaag ttacaaaagc atcagaaagt
     3361 ccaatcgttt ctggtgtaat atataataca ttaaatatta ttgtaatgat tgttttttta
     3421 acaggaatgg gagcttctat tgaaagtaaa aaggatgctt tttggggagg tttattaggt
     3481 ggaatagtgt ttatgactgc tggtatagtt atgtatttgg ctatgatttc agatatagga
     3541 aatttgtata tgaaagaaat accatcactt tatttagcag ataatatatc tccgattgta
     3601 ggtgtatgtt tttcagttgc attaatatta ggtatctata caacagctgt tccactttta
     3661 tggtctgtaa ccaatagaat tgttgaagat gaacatccta aatttaaatt ggtaacaaca
     3721 gtaatagcta ttttggcttg tataggaggt tttttaccgt ttggtaaatt agtaaacatt
     3781 ttatatccat atacaggtta tatggggctt ttaattttag tctgtatggt gtataaagca
     3841 attacatgta gaaaaaaatc aacacaaagt caggaataga gccacttatt aatagttttt
     3901 atttaaagaa attttttata taatccgaat ttaaaaagtc aaaatagtta aataaattgc
     3961 aaaacatgat aatatattta attaaaataa tat
//
LOCUS       DAELJO010000275         3989 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELJO010000275 DAELJO010000000
VERSION     DAELJO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.277"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            108..446
                     /locus_tag="KPB88_003644"
     CDS             108..446
                     /locus_tag="KPB88_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0055878.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(852..2288)
                     /gene="miaB"
                     /locus_tag="KPB88_003645"
     CDS             complement(852..2288)
                     /gene="miaB"
                     /locus_tag="KPB88_003645"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG0055879.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2536..3972)
                     /locus_tag="KPB88_003646"
     CDS             complement(2536..3972)
                     /locus_tag="KPB88_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG0055880.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         1276 a          645 c          434 g         1634 t
ORIGIN      
        1 tatgataatc tatttaataa agtatgaagt aaatgatttt aatgtgcctt tatggtaaaa
       61 ttactaataa gtaataaaat taattaaaat atatggaggt gcttataatg tttgctgtag
      121 gatttgttgt tataattgca cttgcctttt tcattttaag tgtactatta tttaatggaa
      181 aagccagtat gctactagca ggttataact ctatgagtaa gaaagaaaaa gcacgatacg
      241 ataaaaaaaa attatgtaga agttctagca ttgttacttt tattgtctct ataatgctat
      301 ttattatggc gtttttggga tatagaatag agagtggtca aatggacgaa aaaaaaatgg
      361 ttcccttttc tataatattt ataatagtca tactttccag tgtatttttt aatatcctct
      421 actctaacaa aaaatgtaaa aaataaatag ttgtaagttg agcagttaat tataaaagat
      481 tgactgcttt ttcatgtata ttgactactc ttattaaata ttttaacatt taaaatctat
      541 gtaaaaataa tgaatatagt acaaacttct aataatgatt ttacctttgt ggttacagtc
      601 cactatatat aatttaatat gataacaggg aattactaat gacaactagg taagctttta
      661 taaaatataa taaaacaaaa atagatgtac aatgaaatta tatcttatat caaatttatt
      721 tttattaata gcaaaaactt acattagcat aacatatgac aaattcgctt aaaataaaaa
      781 ttttaaatat aatttgttct atagtacatc tattttgatt agtttacata attttattat
      841 tttgtacaat attaattatt tactaatatc ccatttaatg aaaaagttct aggttcagta
      901 attttgacca taactagctt accaattaaa tcatcatttc caccttcgaa atttacgagt
      961 ttattttgtc tagttctacc agcaaactta ttttcattat ttttacttct tccttcaact
     1021 agaacttcaa ctattctatc tttatatcca tcatttattt ctgcagaaat cctatttaca
     1081 gcttctaaca ccctattaaa tctatcatgc tttatatctt ctggtatttg attttccatt
     1141 ttagcagcag gagttccctg tcttttagaa taaataaatg taaaagctga atcatatcta
     1201 actttttcaa ccacatctaa agtgtccaat aaatcttctt ctgtttctcc tgggaaacca
     1261 atcattaagt ctgtagaaaa agcaatacct ggaacttcct ttttagcttt ttctattatt
     1321 tctaaataat attcctttgt ataatgtcta ttcatttttt tcaaaaggct actgcttcca
     1381 cattgtattg gtagatgtaa aaactcgcac accttatcac aatctctaat tgcgtatatt
     1441 acttcatcag atatatcttt tggatgagat gtcataaatc gaaccctttc aattccttct
     1501 atatcattca cttttcttaa aagttctgag aatgaaactg gattttcaag tgtcttacca
     1561 taagaatcta cattttgtcc aagtaaagtg atttctttcg ttccatttgc aacaagctct
     1621 tttatctcgt taattatatc ttcaggagtt ctacttcttt ctcttcctct tgtgtatggg
     1681 actatacaat atgtgcagaa attattgcat ccatacatta tatttacaaa tgcttttaat
     1741 tcaaattttc tattacttct aagaccttct attacttctc catccacatc ccatacatcc
     1801 actaacattt tgtcagaatt tattgattct gtaagtaatt gcggaaattt atatagatta
     1861 tgtgttccaa atataaggtc tacatgttta tatttttttc ttaattcctc tacaacatgt
     1921 ggttgttgca tcatacatcc acatacagct attttcatat ctggattttt tccttttaga
     1981 tgttttaatt gtcctaagtt accatacact tttagctctg cattttctct aactgcacag
     2041 gtgttatata ttattaaatc actttcttca accatcattg acatttggta tcccatctct
     2101 tctaacattg aacataattt ttctgaatca tgttcattca tttgacaccc gaaggtttga
     2161 gtaaacacga gttttctctt tccagttctg ttgaagtatt cttcattttg tctgtgtatt
     2221 tgttctatat atttatcttg ttcttgtatt ttctctatag gtattgttac ttgttttctt
     2281 ttgctcacgt ctattcctcc tagttaattt cttatattac acataacctt aattatatca
     2341 aataagaaaa atcttttaaa gatattatta tttttctagt tgtgttttaa tagtaaatct
     2401 aatgatttat atatactata tttctaaaat ataaaatcat ataattatta taataataaa
     2461 aagatgtttt aaattaaaac ttaaaacatc tttccttaaa taaaatatat gatttactat
     2521 taaatacaat tacatctatt cattttcaat taaataacaa tcatttaata ttaaatattg
     2581 atcttcctca accgttatat atcttcttcc tgaaaaagta tcattagtaa ctattccctc
     2641 tgcattacct aagctatcat ttctaacttc ataatatgca tcagaagaat ttgacattat
     2701 aatatattct ccagcaggaa tttctaatcc aattttatac ataccatttc caaacttacc
     2761 attcttggcc ttctgggctt tagcatactt tgctaatgtc attgttgaat cctcaatttt
     2821 aatatattga ccattcttta aggtgatata tctagtccca ctaaaaatat catttgaaag
     2881 tatactatca gcattacctg tactatcact tgtaacttca taataagaag cataacttcc
     2941 tgagtttgaa gttattaagt attcacctgc tgagatatct tttccaacct tatacattcc
     3001 atcttttata gtctttgtag taggtttaac attttttaca accccagtat taatatctcc
     3061 aattccattt gttatattta aacactgttg tactatattt gaatctatat atcctataga
     3121 agttatcttc ttagcatttt ttaaaatgct tttattgcta ccatcattaa ctaaaaccat
     3181 tggaccatgt tttgataaag aagatcctac aagtgcattt gttaattcat aagctccagc
     3241 tatataaaac tctctagcat ccttataaaa cttatttatt attgctttat tagtttcaaa
     3301 acgagtactc ccccctagtc tagtagattt agtattgttt actaaagtgg tactcattga
     3361 agctgttcct cctattacat aagactttaa accactagtt gaaaaaggta tactttgtcc
     3421 attagtaaga attattggtg ctttatctct tgctgctaca gatgcaatac ttatagcatc
     3481 tgcttctcct ttatatgcat tggttagcat aacagtattg acttttttta ttgaatttat
     3541 ttcttttgca acattataac ttgtttttat tctgtcattt cccttaattc ttacaacttt
     3601 cattttctta gattttaatg aattttcgac agatgtacta atagaatatg ttcctccaat
     3661 tatataaact tttgatacat tttttaatct tgcagatgtc tcagttggta tagtattttt
     3721 ttcagttaat agtatagggg catttaaagc tcctgctagt ccacttgcac ttaatccatc
     3781 tgcaatagag ttactagtat taattaatat agcagtttta tatgcatttt tatctgctat
     3841 tttacctgct atttcatact tattttttcc ttgtattttt tctacagatg atattgcatc
     3901 tactaattga atatttgcaa atacaaaaga aatacttaaa gtcaatgtta atattctttt
     3961 atagaatttc ataataaaat tgtccccct
//
LOCUS       DAELJO010000276         3977 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.278, whole genome shotgun sequence.
ACCESSION   DAELJO010000276 DAELJO010000000
VERSION     DAELJO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.278"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(161..730)
                     /locus_tag="KPB88_003647"
     CDS             complement(161..730)
                     /locus_tag="KPB88_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0055881.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARVHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(802..1410)
                     /locus_tag="KPB88_003648"
     CDS             complement(802..1410)
                     /locus_tag="KPB88_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0055882.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1418..3181)
                     /locus_tag="KPB88_003649"
     CDS             complement(1418..3181)
                     /locus_tag="KPB88_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG0055883.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3224..3691)
                     /locus_tag="KPB88_003650"
     CDS             complement(3224..3691)
                     /locus_tag="KPB88_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055884.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLVFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1332 a          683 c          361 g         1601 t
ORIGIN      
        1 atattacatc atatttttgt aagtttttat tacatttttt ataatacccc tttattttat
       61 aaattaatct aattgattaa atttatccta tagaaattta ttacatttaa aaatatgcta
      121 gcaaataaaa tatctactag cataaagtct atttataata ttaatcaact attaattcta
      181 ctaaatctcc atttttaacg cctgctgcat ttccttcttc catatcaaca tgcatttcta
      241 gattaaagct gtcactagct cttactaata cattttcaaa tactaatgct cttggtccag
      301 atgttttaac ttttactatt tgcttatcag ttacaccaaa tttagcagca tcttcattgc
      361 tcatatgtat atgtctagaa gcaactataa ctccttcttt catttcaact tctccagcag
      421 gtcctactaa tttaacacct ggagttccag ctatatcacc agattctttt attggtgctt
      481 ttactcctaa tgggaaacca tctgctaaag atatttcaac ttgagtatga actctagctg
      541 gtcctagtac tctaactcct tttaaagttc cttttggtcc tactaaatcc actttttctt
      601 cacaggcata ttgccctggt tgagataatg gtttaaattt agttaactca tgacctgccc
      661 caaataatac gtctatatca gcttgactca aatgaacatg tttattagat aatgctattg
      721 gtaatttcat tgaaattccc tccttttaat tatttaaatt attgcaatac aattatacat
      781 aatattacct acaaaatcaa attatattga atcaatatat atctttaaat ttttatatat
      841 tatatcttta aattcttgat tatctatata agatacatca tctgtattgt ttaaatatat
      901 attgtgtaaa agtttagtat ttatactctt aaagaaaaaa tccatcacaa tctgtccacc
      961 tttaaactgt gaatcatatg atttactccc ccccactgca atatacattc ctatccgttt
     1021 cttacttcta tcaattgaag gtttatttaa tacatattta cttgcatata tagcttgagt
     1081 tctatcaaca acagtcttta atttagctga tattgaatca aaatgtacag gacttattac
     1141 tatagttcct tttgaatcat caaacatttt atataatggc ttcatatcat cattaaactt
     1201 gcaataccct gttttttcac aaaatccaca tgctgtgcaa tattcaatat ccatattgta
     1261 gatattaaat attttacaac ttaatccaca ctcttctagc atagctttta cttcatttgc
     1321 tataaaactg caatttttat taattcttgg acttccattt attattataa tctctggact
     1381 tttaagtcct tttttatcgt ttgaatccat aatcaatcta cctatccttt aacataacta
     1441 ttaaaatatc atcatcataa ttatcttttg atgagaagtt ctttaaatcc aattttagat
     1501 tttctactat taactgttga tttaatctaa aattttcttt tagaaagttt tctaatcggt
     1561 ttataccata ctcttctttt gatgaatttt ttatctctaa aattccatca gtgtacatac
     1621 ataccattgc ataatcttcc attgaaagag tattattttc ataagtagca tcttccatta
     1681 ttccaatagg tatgcccttt ttacaattta aattattttc tattgtgcca tctttcttta
     1741 caactacagg actatagtga cctgcattag acacagtaat catattttct ttagtatcta
     1801 taatacctat caaacaagtt gtaaatactc ccatcttgtc aaattcatca tataacatcc
     1861 tatttaaatt tgtcattatt tctcttggtg tattgtattg attacataat actttaaaag
     1921 caccttttat cattgcaaca acataatttg ataccattcc atgtcccatt acatcagcaa
     1981 caatataaac tatatgttca tcatccactt ttgtagcata ataaaaatct ccacctacta
     2041 cccttgctgg tttatggtaa aaataaagtt ctttctcatc attacaatcc attttacctt
     2101 tatccataat aagctgttgt tgtttattta atatgttcaa ctcgttttca agaactttgt
     2161 gttttacttc cttttcatta tgttcatata attgcatacc aacagaaact tgtttggaca
     2221 atatactaag aatatttaaa tcatcactag tatagttttt gctatttgca caaactatac
     2281 atcctatagt atcattttta tcctgaagtt tgtaatatat ataactttcc aaatttattc
     2341 ccttttttga gcatggtttt aaattattta gatacccccc caatgactct ttctttaaaa
     2401 acatatcaat cataggatat aaatttgttt tctttttttg ctcaacatta taacaactaa
     2461 ctatagcatc cttattatga tgactattta taattattaa agcttcttta ctattagtaa
     2521 tattacacgc ttgtttactt aaattttcca aaaagccatc aatactctta ttaaggtata
     2581 gttttttagt agcttcattt atggcactta ttgagcctct tagtttatca attaaaattt
     2641 gtacattatt atttttatta gttatttcta ttgataaact taagagtgat gcaacagatg
     2701 atataaaatc tatatcttgt tctgttatat aatcatcctc ttttaaaaaa caagtcataa
     2761 atccaacaac tacgttattt attataaaag gaaaaactat tctcccaata taaccttcag
     2821 cttttgctaa atctcgttca ccttctgctc tagaatcttc aaatacattt tccacatata
     2881 ttatcttttt ttcatttaca gcctcatgta tatactctgg atatgtgtca aaatcaattt
     2941 ttgcaccttt aagactattt attggaaaaa tttgaggtac atattctaag gtttgcgaac
     3001 atactaagta ggcatattta gaatcatttt tataaaatag attcacacat gcctttgtag
     3061 ggtgaacaac ttctaacatt ttttctatta tgtcatcttt tatattaaaa aaatctgtag
     3121 attcagtcac catagcggat atttccacta aattcttcat tttataactg ccattagtca
     3181 tactcaattt ctccccctat atattaaact atttattagt attttaacat acattcttta
     3241 tagtaaaaac aaaaacaacg acaaaactag caactaatat ccagttaaat cctgtcaata
     3301 atcctataag aagtaatgta attaatgcat ttagacctgt tctccctttt tcatccataa
     3361 aagttatttt agagatgtcg atattatttt tcacattatt tatatgctct tttacttgtt
     3421 cttccatatt tcttttatat aatatataaa ctacaccaac taaaaacact cctataccta
     3481 aaaccatgtt taaattaaaa gctaccataa gcaatattga accaaaaact aactttaaaa
     3541 gcttaaaaaa tacactatct atctttagag ttatatttct gatttcttta cattcatctc
     3601 ttaaagaata tccacttgaa tctaagttat taatctttgt ttttacaatt tcagaataaa
     3661 aaagctttat tatcccattt aacattttca tcactcctaa atattttaca tatatttgta
     3721 agttttacaa ttataatatt ttttatactt ggtaataata aaaaataaca aatattttat
     3781 gatttagtat ttattgtaaa taagttattg aagctttatt agttaagtat agatttttaa
     3841 taaatatttt tataattaga aggaagtggt tttgtgcaaa tagaacaact cgatttagaa
     3901 acgagaaata aaatatactg ctatactaaa aaaattctta taaaatatca aaaaggcata
     3961 acatctggaa aattaac
//
LOCUS       DAELJO010000277         3973 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELJO010000277 DAELJO010000000
VERSION     DAELJO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.279"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            188..1000
                     /locus_tag="KPB88_003651"
     CDS             188..1000
                     /locus_tag="KPB88_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG0055885.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1089..1280)
                     /locus_tag="KPB88_003652"
     CDS             complement(1089..1280)
                     /locus_tag="KPB88_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055886.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1551..1790
                     /locus_tag="KPB88_003653"
     CDS             1551..1790
                     /locus_tag="KPB88_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0055887.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2099..3718
                     /locus_tag="KPB88_003654"
     CDS             2099..3718
                     /locus_tag="KPB88_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0055888.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT         1483 a          471 c          565 g         1454 t
ORIGIN      
        1 gaaataatca ctttttcaag aatgttataa tttttttatt gtattctttt tgctatagtc
       61 tatgaataaa tcaatagaaa atcttctaag ttgcttttaa ataatgactt tatggttcaa
      121 ttgtactaaa atagtatatg taggttacaa ataatctaat aaatattaaa gtttggaggt
      181 aatagatatg gcagattgta attcatgtcc atcaaaggga aattgtaata gtcaatcaaa
      241 ttgttctatt gaaaataacc ctaacaataa atttggaaaa ataattggtg ttatgagtgg
      301 gaagggtgga gttggaaaat caactgtaac tgctttactt gctaataagc taaacaaaat
      361 gggatataag gttggtatat tagactcaga tataactgga ccaagtattc ctagacttat
      421 gggtgtaaaa aatgtaaaag cttattctga tggctcttat atttaccctg tagaaaactc
      481 aaataacata aaagtaatgt ctataaacct tatgatagat gatgaaaatg aacctgttgt
      541 ttggagagga cctttacttg gaggggttgt aaagcaattt tatactgatg tactttggga
      601 agaattagac tatttattaa tagatatgcc tcctggtact ggagatgttg ccttaacagt
      661 tatgcaatct attcctataa gtggaatagt tatggtttca gtacctcaag atttggtatc
      721 tatgattgta tcaaaagctg ttaatatggc taaaaaaatg aacataaatg tccttggagt
      781 tattgaaaat atgagttata tccaatgtcc tgattgttct aaaaaaatca agttatttga
      841 aggagaatct actgaaaagt ttttagatga tttagactta gagcttttag gagaacttcc
      901 tatgactaaa gaaattatag atattactca taatggagtt acagaaatta gtgatgattt
      961 agattcaata ttgactaatg ttgttgaaaa aattaaataa ataaaaattt tataatttta
     1021 tatttatcaa aaaaagaggc ttgtatttat tttataatca tacaaataaa tataaacctc
     1081 ttttatgttt atctatgttt taaatacaca aaatattctt ctacttcatc tccatttttt
     1141 acttttacta aatattcatt ataatgagca ttatatggct gtgtcatttt gcattctatt
     1201 acattttcta tgtcttctaa ttctaaaagt tctacatcct ttaaaaattt tctttctatg
     1261 agttccatta atctttccat aatatatcct cacttcccct ataattaaga taaactatat
     1321 atcctcttaa tgcagagttt atctgtttat tatattatat ccaatatatt acagtttcta
     1381 acatcattca taaattattt taacaaatag agattgattt tttaataaat atgccttgat
     1441 aaaatgaaac atagaccaac tcttagtttt atgtaaagaa aacttaaaga aaactataaa
     1501 ttatattcac aaaaattact cagaagatat aacagtaagt aatatatgta atgaattaca
     1561 acttaataaa agttattttt tctttgttta agaaaaaaag tggatatact tttactaatt
     1621 ttttaaataa agttagagtt gaaaaaagca aaaagtatat attaaaaaaa gacttgtcta
     1681 ttttagacgt tgctgtacta attggattca atagtcaaaa ttactatagt ataattttta
     1741 aaaagtttaa taactttaca ccaattgaat ataaaaatat ttataactaa aaaatatcat
     1801 tctaaaagga tgttattttt ttacatattt tttacaatta tagttaattc tttgttaaat
     1861 tgattgtaca taaaatacta aaaatctaag ttcaagtatt taaattgcta tatatcataa
     1921 attttctaaa attaacaaca acttaaaaat ttaacattct cttaacgtat acttaacaaa
     1981 acattaatat ttttattgac aaaatttgtt attctatatt atggaaacaa taaattttag
     2041 ttaataaagt ttataattca gataaaaaaa ttattaattt cttaagggag gaaatatcgt
     2101 gaatatagta attagcctta tcggtggact tggacttttt ctttacggaa tgagtttaat
     2161 gggagaaggt ttacaaaaat cagcaggaga caaattaaaa aaaattattg aactcttaac
     2221 aagtaatgtt gttatgggtg tactggtggg tacagtcgtt acaggtatta tacaaagttc
     2281 aagtgctact actgttatgg ttgtaggatt tgtaaatgca ggtataatga atctaagtca
     2341 agctattggt gtaataatgg gggcaaatat aggtactaca gttactgctc aattagtttc
     2401 atttaatctt gaaggaattg caccaattgc tttaggtata ggtattttat tctatttatt
     2461 tacatcaaat caaaaaacaa agcatttatc tgagatactt ataggttttg gtatattatt
     2521 tacaggaatg gagtttatga aagatgcagt agctccactt gctgaataca aagcattcac
     2581 tgatgcactt ttatatttta gtaaaaatcc tgtattaggt atattggcag gatttgccat
     2641 aactggtatt gttcaaagtt caagtgcatc tatgggtatg ttaatagcac tagcttctca
     2701 aggaatactt cctctatcat ctgctttacc tattctttat ggagataata taggtacttg
     2761 tgttacttca cttttatcaa gtgttggtgc aagtagaaat gctcgacgtg cagctgtaat
     2821 gcatttaagt tttaatgtaa ttggtacaat aatatttatg cttgttttaa ataaacctat
     2881 atcagccata gttactcatt ttgacccaac tgatacagct agacaaatag caaatgcaca
     2941 tactttattt aatcttacaa atgttataat attattacca ttttctaaat acatagttaa
     3001 attagcaaat agacttatac ctattaaaga aactgaatca gaaatagtaa ataatactaa
     3061 gtacttagat gaaaggatgt ttagcactcc atctatagca cttggtaata ctgtacaaga
     3121 agttgtaaga atgggtcata aagctacaaa ctctttggaa cattcaattg caggattttt
     3181 aaataagagc aatgaagata ttaataaagc ttttgagtca gaaaaagttg tcaataagtt
     3241 acagaaagat atattgaact atttgttaaa actttctaaa gaacctctta gagatgatga
     3301 acgctttaga acagatttgc tttttaatac tgtaaatgac attgaaagag tctctgacca
     3361 tgcagaaaat atagctgaat tggccatgag tgttaaggaa atgaacatta gtttttctga
     3421 tagtgcgata agagaaatat acgaaatata caataagaca attactaatt ttaaggatgc
     3481 cttaatagtt ttagatgtta aagattttga gttagcaaat aaagttttag aagttgaaaa
     3541 tgaagtaaat tatttagaaa aaacttttag aaattcacat atgattagac taaataatgg
     3601 aagttgtaca attgatgctg gtgtacttta tcttgactta cttactaatc tagaaagaat
     3661 ttctgaccac tcaacaaata tagtaaaaca agtactaaaa cttaaacaaa aaatatagtg
     3721 attattttta tatataatct taatcaatga gaagtaagaa gctatagata atgcttatat
     3781 catctatggc ttcttatttt ttatgtaata ctcaaatttt tatagtattc tcttattatt
     3841 aaacaatttc tgcctagaat attccatata tatatgactt ctttcataaa acttgaactg
     3901 cccataacta aagtcacaag tatacataac tattttttaa tcaattataa ccaatatttg
     3961 ttttatttat ctt
//
LOCUS       DAELJO010000278         3964 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELJO010000278 DAELJO010000000
VERSION     DAELJO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.280"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..426
                     /locus_tag="KPB88_003655"
     CDS             <1..426
                     /locus_tag="KPB88_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG0055889.1"
                     /translation="NIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYK
                     KNINIEILLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGS
                     EAISIWTKNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            413..1294
                     /locus_tag="KPB88_003656"
     CDS             413..1294
                     /locus_tag="KPB88_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0055890.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVITDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(1520..2875)
                     /locus_tag="KPB88_003657"
     CDS             complement(1520..2875)
                     /locus_tag="KPB88_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0055891.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1530 a          576 c          489 g         1369 t
ORIGIN      
        1 aacataaaag gagataaaaa tatattaaaa aaagcaatag aaatgataaa aaatgcaaaa
       61 caagaaatta taatttcttg cccttatgaa gaaattcttg aattaaaaaa agaactccta
      121 attgcttaca aaaaaaatat taatattgaa atactacttt tagaaaacaa aagtctgaaa
      181 ggtcttgata atattcatat tcatggaggt aataaaacta cacttgaaaa taaaatgctt
      241 tacaaaggaa ttattttgat tgtagataat gatgaaatat tgacaggaaa cttatcagaa
      301 ggcagtgagg ccatttcaat ttggacaaaa aactctaaca ttttatatat tggagttcaa
      361 tacataaaac atgaaattta tatttcagaa caaataaaag gaggttataa aaatgaacaa
      421 aattaagatt aaacaagttg atgcttttac aactatacct tttggtggaa atcctgctgg
      481 tgtcattact gatgcctcta acatttctga tgaaattaag cagaatgtgg ctaaagaaat
      541 gaatttatca gaaactgcat ttgtttcaga gtcaaatatt gcagatttta aggtacaatt
      601 tttcacacca ggtttagaag ttgatttatg tggtcatgca acaataggta ctttccatgc
      661 acttttggaa gaagggaagc ttaatactaa taagagtata ttttatcaag aaacaaaagc
      721 tggtgtatta tctgtagagc ttaagaaaat aaataatgaa aatatattta tgatggaaca
      781 gaatacacct gaatttgaaa atttagataa ttgcagaaaa gaacttgcac aaatgatagg
      841 tgtgcatgaa gatagacttc ttgaatatcc tattataaag gtaaatactg gactttggtg
      901 gcttgtattt ggtctaaaat ctttagatga tttaaaaaat atctctccag atttaaataa
      961 aatcaaaaaa tttagtggtg ataataatat aattggtata actccatttt gtatagaaac
     1021 tatagataaa aagtgtgact accatttaag atctatagca ccatatgttg gagttacaga
     1081 agaccctgta tgtggaactg ggaacgcttg cgtatcatct tatatagtac atcacaatat
     1141 tttagataaa actagtttta ttggtgagca aggtaacttt ataaacagac ctggaaaggt
     1201 atatgtaaat ataactagag aaaacaatga cataacttct gtaaaaattg gaggaagtgc
     1261 ttatactatt ttaagtggtg aaattatgta ttaatcccat acctaataaa aacaaataaa
     1321 gatactagct aactaaagct agtattttta tttgttattt ttatttaata atcatactct
     1381 aacaaatact ataattatat atatcataat attgtattta gaagtaaaat tttattttta
     1441 ttattaatta gttataaatc aatctaatac aacataatta cagtagatta acctataaaa
     1501 cataataatt gcttactgat tatttatcgt cctcaacaag tctacttaat gtcttatcag
     1561 ataagaataa taatatcgct gcaacaaata gtattactgg aattatagta tatactttaa
     1621 tcatatcaaa tttaagagta aatgcatata tatatccgta tcctttacca gctataaaag
     1681 tagcaaatgt ccatactccc ataagaacac ctagaagctt cttaggtgca tacttactta
     1741 caaaagagtt accaagtggt gagaataaca tttcaccaag acttaaaaga ataccaaaca
     1801 tgattatcca tagtatacta gctttagcag tttcaggagc tccaacccct cttgaaaatt
     1861 ctgcaccaac aagcattaag aatgcaagac ctaagaatat taatccaaga cctgtctttt
     1921 taaataaact catatctcct tgtggtctac tagcaagttt aaaccataat gctccaagaa
     1981 ctggtcctaa tactatacat acaagtgaat ttaatgagtc aaaccaagca agaggaacat
     2041 caaatcctcc aacattcatg tttacaaatg ctgcaccata atcataaact gcaagataag
     2101 ttagatacca gaatacccag aaaattacag agaagaatga tataagtata atagcccaaa
     2161 ttctcttctt atccatgctt gtaagaggag acttatcttt ttcttcaact ttttcagcaa
     2221 ctacaccttc tttaaaaggt ctctttcctg cattcccaag gaatctccat ccatatatga
     2281 accatattgc acctattaca caaagtacag ctgctataaa gaaacattga gaaaatccaa
     2341 gtacaccatt ttttgcaaat gttttaaggt aaagaatacc aactgctata gtaccaataa
     2401 aagaacctat attaacaaaa gaatactgta ctgaaaatgc tgtatcaagt tcttcttgac
     2461 tatcaaaaag ctgtccattt accgcagata cgttaccttt aaagaatgca gtaccaattg
     2521 aaactaagat tatcattgca tttaccatag aaactgtagt tgcaatagaa ccaagatagt
     2581 atcctacccc catgataagc atacctatag gtatagtata tctagctcct atccatctat
     2641 ctgatatata tcccccaatt ataggtgcaa ggtatgtgaa tgcaacaagg ttggcttgca
     2701 ttatagcagc ttgtccttta tctattccaa gaccaccatg aactatagct gttgtaagga
     2761 atatatatat taagtacttt gaagcataat atgctgctct ttcaaatgag aaagttgttg
     2821 agcagatata aaatcccata ggaaatttac ccttcttttt tactgttgag ctcataattt
     2881 taaatccttt cttaattgta tttttatttt ttacgttttg tccaaattct ttcttttatg
     2941 aaagatataa tattattatt ttgtaaaaca atttgtaatt attattaaga taattgcgca
     3001 tttatttgta gagtacatat tctcatggaa taaaattaat acacttctta atcataaatt
     3061 taaattatat aaattcacta ttttcagata tagttataaa ttaatatatt ctcatctaat
     3121 accatgaaaa aaccttcgcc atatgcaatt cttatttttt acgtgatagt attttacaaa
     3181 ctaaaatccc ccttttgtag caacttatgt aattacattt tagtgctttt ttaaaggagt
     3241 ctcgaataaa gcaacatata ttttgttcaa ataatgtttt tatttatttt ttaaatttat
     3301 atattacttc acccaaccaa ttctcaactt aaataactaa actactatct attcacagat
     3361 taggcttatt tcttacacat tagtttataa taaacactgt gaatctaaac ataaaaactg
     3421 ttattttaaa gtaattaccc atatattcgc tttgatacta tttattgagg ttatactctt
     3481 tatgtataat atattattta taaaatacct caaaatatat tattcataaa atacctcaaa
     3541 ctgaaaactt tttcacgaat atttattttt cacctcccct tttttatttc accatgtaaa
     3601 tcgtaccata aatatgcact tatatgcaag aaaaaaatct attaatcgac agtaaataat
     3661 tagaaaaatt caaattatat ttatcaagta tattatataa aatatttcct atgaaaatat
     3721 aaattaaatt aatgttcaga attgttttta gatatgaaat cttgattttt ttattacttt
     3781 acttatttgc tcaaatatat ttttgtatgc agtatataat aatcaaatta tacacttctt
     3841 ataactgcat tgatttgaaa ataatattaa taattaatag aataaaaaat ttaaattaaa
     3901 tttaaaaaaa tttgttaagt tgattaaata cttttttatg tatttaatta attttataaa
     3961 ttca
//
LOCUS       DAELJO010000279         3954 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.281, whole genome shotgun sequence.
ACCESSION   DAELJO010000279 DAELJO010000000
VERSION     DAELJO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.281"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(326..1393)
                     /gene="rsgA"
                     /locus_tag="KPB88_003658"
     CDS             complement(326..1393)
                     /gene="rsgA"
                     /locus_tag="KPB88_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0055892.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVILDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVYRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1390..1791)
                     /locus_tag="KPB88_003659"
     CDS             complement(1390..1791)
                     /locus_tag="KPB88_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG0055893.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLISIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2584..2680
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2734..2910
                     /locus_tag="KPB88_003660"
     CDS             2734..2910
                     /locus_tag="KPB88_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055894.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3320..>3954
                     /locus_tag="KPB88_003661"
     CDS             3320..>3954
                     /locus_tag="KPB88_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055895.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEADFKKYKLEVKKDDKYIYKKNYENYVVLIIKKDEQKLYSLIWN"
BASE COUNT         1483 a          590 c          499 g         1382 t
ORIGIN      
        1 aaattagcaa tattgcagtt ttgctcttaa gaaacatctc tatcatacat aagcatatac
       61 tctattatgt attagtataa atctgtcttt tcttatctta cataagacct ctttataaag
      121 gagactatcg taacaaatat gtatacatta tcacatgtat atatcatcat ataaaaaatc
      181 aaactttatt aaaaaaaata ataaattgtt ttaaataata ccacaaatca aatttgccat
      241 caaaatcaca ttaaagtaac aaattaatgg caaatttaat atattaatag tatatagact
      301 catataatga acaaaaaaac tacacttatc ttctaatctt cttatttatt tttgagatat
      361 ttttaaattt cttttctttt tttgctaagt attcgctttt attgtctgca aatactgctt
      421 cagttttaag cctacgatag gactcccatc ttttaggcga aagttcttga tgagcaatag
      481 cttcccagat agcacagcct ggttctgagg tgtgagtaca attacgaaaa tgacattttc
      541 cgaaataagc ttctatatct gcaaaacttt tatctaaacc ttccgaaaca tcccacattc
      601 ccaactctct catacctggt gtgtcaataa caataccacc acctgaaatt aaaaatagct
      661 ctcgtctagt cgtggcatgt cttcctttat catcatctat gcgaatttcc tttgtattta
      721 aaaattctcc acccaacaga caattaatta atgaagattt tccaactcct gaagaaccaa
      781 gtaaggcaac agtacagcct ggtttaatat acttagaaat ctgtttataa ccatctggct
      841 ctatagatga ggtaacaact acatctacac ccaaggctat agaattaact acttgtaatt
      901 tacgatacac attgtcacat aagtctgatt tagtaagtac tactactggg atagctccac
      961 tttcccatgt taaagacaaa tacctctcca atctatgaat gttaaaatcc ttattcagtg
     1021 acatgcacaa aaaaacaatg tcaatattag ctgcaaccac ctgctcctca tttacctttc
     1081 cagcagcctt acgcacaaac aagctttttc tatgaagcac atgatgaata atagcattac
     1141 catttttatt attgcaacga tccaatataa cgaaatcacc aactgtggga aaatcagatt
     1201 gtatagtagc ctcaaaacgc agctttcctg aaacctctgc aaacagtaca ccatttacac
     1261 atacagcttg ataaaagttc ttttcctgcg agataattcg agcaaggtat aaatcatgat
     1321 attccatact ttgagtaata aaattcctat ttagaccata gtcttgtaaa ttgatttgtt
     1381 tcataccatt cattctgttt cctccttttc caacttttca atgattcttt ctagtgggaa
     1441 agggggctgt gccagtggtt ttaatgcaat tgatattaat ttcactgaat tatcatcagc
     1501 tgcaatacga ttagaactaa gatgtccaca gcgtgtacaa cgatgaataa ttgcccactc
     1561 accatttttt ctcacccaca ctccaatagg ttccatatta gcatcacaat ctgcagcacg
     1621 gtcaccagga atgatatcta catgaagact atgtaaacaa tgtggacaat gatttctatg
     1681 ttgtgtacca gctccttcag gaactacaag gcaaccacaa tttttgcaaa taaagttatc
     1741 catacaggaa ttttcctgat aataagctcg ttttctattt ttataattca tttttatttc
     1801 ctccatatcg tgtattttct acaaaaaaag tccacaggta tttatatctg cctttttatg
     1861 tggtatacat atttacctac acaaaaacag aaaacccctg tggactagaa aatttaatat
     1921 cttctatttc aacataatta tattcaaaat aatactgtgt aaaattaata aaagtgtagc
     1981 agattaaagc ccactacacc acaatttaac aaagtaatat ataatattat tatttgttgt
     2041 ctaatgttag ttatctataa aggcagagta agacacaaca atcaaatgta tcaaaaatac
     2101 acttaaaaat tctgattaaa cagaacccat atgcgtctcc aactatttat ttgttggtta
     2161 ccattatata taactctgac atcaggcaag acactccttc actaataatt attgaaattt
     2221 tatcataaaa tataagtatg ttcaagtata atttaatata ttcatatatt tatgtttgtc
     2281 tcattttctt attctataaa atctagtggt attttaaaat ttaatgttca cctagtatta
     2341 ataataaaaa ttaactgcta ttatttgttt tctcatttta tgcctattgc tcaatttatc
     2401 tcacttaaat taataacaac tgtcttttca tacaaaacct acaatcaagt ttttccatat
     2461 attccacatg catagtctat ttttttgata taaaataacg ttcatatttg taaatttatt
     2521 taattttcaa ataagtatat tgaataataa gcaattctat tgtattattg taattgtaga
     2581 ataaaactaa atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaacgc
     2641 ttataaaata aactatgagt tatgtcagcc agttgccaaa aagagatatt ctttttgttt
     2701 ttatgaaagt tttttagggg gataataata tatatgttta gagataaaat ggatagatgt
     2761 acacatatgt tgactgctta tattggtagt tcatatgatt attgtgattt tatagataca
     2821 caactagatg attttatact agagtacgga gaaaatgtgg tagaatcttg tttgcatcaa
     2881 gtgatggtat tagtaagtaa gtataattaa atagatttgg ttttctagag aacccttttt
     2941 agttttatct actttaaata gccttatgtt agaaagctaa ttaagataga tttctagtat
     3001 tcataatttt attttttatt taaaatacat ataataaaga tgtaaatttg tatcaaataa
     3061 taaatatgag aacttaaatt ctcttatcta tgtgaaaata aacatctctt aaaatgaaat
     3121 tctgtaaaca taaatgattt tttagtttga aggattagat taacaaaatt ataaatttgc
     3181 aaataagcta tattaaaaaa tataataatt ttttatataa cttattcttt tataactttt
     3241 aattttaaaa ggggagaatg tatataagag tattcaatgt actagaaaaa atcaaaagaa
     3301 atattagaag aaatggatat tgagtaatca aagaaaagat tttgatatta caaatgatat
     3361 gtttcaagaa agcattccta tcacaacaaa aattattctt gaagacatgc cttcaaatga
     3421 tgaattaaat catgtttttt caaaaggttg tgaacgaaaa atgaaaaaga gaaaaattgt
     3481 attaatcaca ctactattaa taggtgtttt gttattagga agtatcctat ataatctttt
     3541 tttaggaaaa acagctaata tttcaatgct aaaggaatct tggaattttg atataccaat
     3601 acctaacaaa gaaattgaag tatttgatac tcaagatagt attaatggag atggacaaag
     3661 ttattttatt caaggatttt ctgaaaaaaa tttcaaaaaa gtttttaatt taaaaggtgg
     3721 tatagttgta agcaaagata atattaatga aatagaaaaa tatattgata aatttaaaag
     3781 agattctgtt aacattaata aaagtaataa aaataaaatt gaagcagatt ttaaaaaata
     3841 taagttagaa gttaaaaaag atgataagta tatatataag aaaaattatg aaaactacgt
     3901 agttttaatt ataaaaaaag atgaacaaaa attatacagt ttaatatgga atca
//
LOCUS       DAELJO010000280         3937 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.282, whole genome shotgun sequence.
ACCESSION   DAELJO010000280 DAELJO010000000
VERSION     DAELJO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.282"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(181..3756)
                     /gene="cbpA"
                     /locus_tag="KPB88_003662"
     CDS             complement(181..3756)
                     /gene="cbpA"
                     /locus_tag="KPB88_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG0055896.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAEED
                     SNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAEDPTSVGDFKI
                     SGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKT
                     ASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRD
                     IEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNT
                     ESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAG
                     IGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTN
                     RGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLE
                     KKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQGVLYLWFSDQ
                     FKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDTPPYLIIKRY
                     DGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDTNLG
                     SDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTETSLVVEESK
                     AKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSE
                     SHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVD
                     GDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDK
                     NIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNG
                     GNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNGKPDPD
                     NGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGD
                     GKPDINIDIDGDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTD
                     ESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1094 a          860 c          434 g         1549 t
ORIGIN      
        1 tgatatcaat tccattaatc tcttttaata aaaaattctc ttttctatat gaaaattatt
       61 tcaaaacttt taagttaaat acaaaaatga ctataaatag atttttattt acacaaatct
      121 atttatagtc actatagttt atccaataaa ataataaaat aaatatgtac acaataaatg
      181 ttatttacgt cttaagtatt tatttgttag atttttaatt aaaaaaaaca agctaatgaa
      241 cattaatgct aaatctagca ttatattact ttgatctcct gtttgtacat tttgacctac
      301 attactatct aaactttcat ctgtatctat atttatatct ggttttccat tcccatctgt
      361 atctatattt atatccggct ttccatcccc atctgtatct atatttatat ccggttttcc
      421 atctccatct atatctatat ttatatctgg ttttccatct ccatctatat ctatatttat
      481 atctggtttt ccatctccat ctgtatctat atttatatct ggttttccat ctccatctgt
      541 gtctatattt atattcggtt ttccatcccc atctgtatct atatctggtt tgctatttcc
      601 tccattatct gggtctggtt tactatttcc accgttatct ggatccggtt tgctatttcc
      661 tccattatct ggatctggtt taccattgcc accgttatct ggatccggtt tgctatttcc
      721 tccattacct gaatccggtt tgctatttcc tccattacct gaatctggtt tgctatttcc
      781 tccattacct gaatccggtt tgctatttcc tccattatct ggatccggct tgctatttcc
      841 tccattatct ggatctggct tatttagatt tggattacct gtatcataac ctcctgagcc
      901 agtatctaca tttttatcag gcttccaata atcatctact ccatcattat ctgtatctat
      961 tgctggacga ttataatctg tatcagggtt tccatctcca ttggtatcta tatttttatc
     1021 aggtttccaa ataccatctc catttccacc ttctgtacta ggcttccatt cacctgtatt
     1081 atcagtatct atgttaatat ctggcttcca atcaccactc gtatctatat tgatatctgg
     1141 ccttccatca ccatcagtgt ctatatttat atccggtatt ccatccccat ctacatctac
     1201 atttgtcata tctaacttta acttcttttc atcctcacta ccttcaatgt aatttatatc
     1261 atctccaata tattttacag taatagtttg ttcagtagta aaaatatgat gattgctatc
     1321 taaaggtctc cattcaatag atgcagtact ataattatga ctctcactac ccttattatg
     1381 agtttttaat tctactggat ctttatattc ctttccatta atatagaatt gtacgtaacc
     1441 tcttggagag gcacaatctg gtccttcttt cttatctggt gaaatagtaa catttaatgt
     1501 aaacgtatct gttggtttaa aaacatcagt tttagcttta gactcttcta caactaatga
     1561 agtttctgta tcagcaggaa taatttctgc cttataatat gctctatgcc cccaataagc
     1621 ttcattaaaa ggatatacat ttgaaaactg tgttgatgta atgcttaatt gatattttcc
     1681 agcatcaact tgctttgtaa ggtcatcact tcctaaattt gtatctggtt ccattgtttc
     1741 ctcatttaaa cgttgagaac taatagtcat aaatttttta tctgtgagtt tttcaccttt
     1801 aggagtaatt attggaatac ccccatcttt taatatccaa tctaaaaata atttttctac
     1861 atcttcttca aatggtaatc catcatatct tttaataatc aaatatggtg gagtatcatc
     1921 aatagtaaat tcttcatatt gctttaaaaa ttgagaacct gaacctatat ctttaacata
     1981 aaaatcatct ggttttatat ttcctccagt aattttatct ataacaacta aattaacaga
     2041 tacttcagat cccttaaatt gatcagaaaa ccataagtaa agaacccctt gatcatctgt
     2101 ataactaggt gctccataag gataaatttt tccatcaatt agcattttaa aatcatcaac
     2161 taatgtagta ctaactttat cttgtcctaa atactttaaa gatattttat tcataaaaac
     2221 ttttgttttc ttttctaaat caccgtatgc aactgctcca cctaaacttt tgaatgttgt
     2281 tgcttttaat gaaccacctg ttactataac atctcctgtt gtagcactta ggtcaaaaat
     2341 ccaatctccc actcctgtca tataaggtaa aatcgttcct ccagttacaa gtatacgata
     2401 tcctctattt gttcctggat taacacatcc atccccaaat gcactaccat gtgcacctcc
     2461 atatgaatag gaaagtccac cattaattgt aatatttcca ctataaccag aacctgtgct
     2521 aggatctact actgagggac ttaatgcatt ctttgttgtt gtatagtatc ctccacctat
     2581 tccagctcca tggtatgcac ctttagcagt tatagtacca ccattgattg tgatatctcc
     2641 accactacca ttaccgcctc cacctattcc agctccatat cctgtattac gactcgattc
     2701 ttgacctata gcagttatag caccaccatt aataataata tttcctccac tttcagtatt
     2761 tccaccgcca atacctgcag aataataatt tccaacaact tttaaacttc ctctattatt
     2821 actatctagt agtgtaaggc gatctccttt ttttaacttg tctccatttt ctagagttac
     2881 atctctagga atttttccac cctcaggaac tataaactct ccttcaatat ctctatttac
     2941 aacagaatca tcaataatta gagtagtacc ttttcctgct ctcatacctg gataatatac
     3001 atcacctgac tctagcttgt tttcactacc atcttgtaat ataacatgtg catatgcacc
     3061 accatcttta tctggtgtta aagtaaatgg gctattagat gcagttttaa tatttactcc
     3121 atcaaatatt aattcagctt ttacatcttt aggtataaca atcctatgtg ttgttggagt
     3181 atttggatta ttattttgaa ttgtcatttg ttttccacta agaatagtta aaacgccact
     3241 tgcaaattta aaatcgacat ttatttctcc accactaatt ttaaagtctc ctacagatgt
     3301 aggatcttct gcagtggctt cttccacaac taatgcattc atatcttctt ctgcatttcc
     3361 tgcattagaa tcttcttctg catttcctgc attagcatct tcttctgcat ttcctgcatt
     3421 agcatctcct tctgcatttc ctgcattaga atcttcttct gcatttcctg cattagcatc
     3481 ccctcctgca tttcctgcat tagcatcccc tcctgcattt cctgcattag catcccctcc
     3541 tgcatttcct gcattagtat ctccttctgc atttcctgca ttagtatctc cttctgcatt
     3601 tcctgcatta gtatctcctt ctgcatttcc tgcattagta ctttcttcta tagtatctgc
     3661 gaaagtattt gacgcattaa atgatgctac tgatccaagt cccattgaga tacttaaaat
     3721 cattgatagt gctttctttt tacatttttg ttgcataaat tccccctccc cttaataatt
     3781 tttaatatac actgtataca tccttatata cactctaata attcttaata tacactgtat
     3841 acatccttat atgcactcta ataattttta atatatactt aaaacttata tttgtcaatg
     3901 aatatactgt ttttttcaac cttatatatt ataagta
//
LOCUS       DAELJO010000281         3927 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.283, whole genome shotgun sequence.
ACCESSION   DAELJO010000281 DAELJO010000000
VERSION     DAELJO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.283"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(228..608)
                     /locus_tag="KPB88_003663"
     CDS             complement(228..608)
                     /locus_tag="KPB88_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0055897.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(809..2002)
                     /locus_tag="KPB88_003664"
     CDS             complement(809..2002)
                     /locus_tag="KPB88_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0055898.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2065..3321)
                     /locus_tag="KPB88_003665"
     CDS             complement(2065..3321)
                     /locus_tag="KPB88_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0055899.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1425 a          727 c          415 g         1360 t
ORIGIN      
        1 ataaagaata gaaacaagtt ctagattact atagagatga catatcaaaa tatttttaaa
       61 aagcaattta agtattagaa gtaattgaag ttagtaaata taaatgcgtt tacttttaag
      121 tattgcttaa gggaataaaa aataaaaata aactctgcaa ccatctaaaa atagttgcag
      181 agcttacaaa accaaaatta tctaaattgt aataaaaata ataacattta tacataagct
      241 tcttcccaat ttactaccca gttttctaac gactttatct tttcctctgt tagctcctct
      301 tcgtgaaatg atactaataa attccttata aaattattct tcaaaagatt ttgcacattt
      361 cttgatataa actctaaata tgccttttct ttgattgcaa ctgtataata catacacttg
      421 cctgtttctt gaacatataa gaatcttttc tttgatagtt ttgataaagt ttttaaagct
      481 actttttctg gtaaattatg ttcttctttc atataatcaa taatctcata tgaattggtt
      541 tttatatcta aattccaaat aaatttcata ataactaact ctaattttga taattttttt
      601 aaacacatag ttatcactcc gcttctttat ctatataatt aaatattaca ataataattt
      661 cacaaaatca attctaataa tcataacata ccttaaaatc tgttcttaac ttctaccttc
      721 ttaatctccc aaatgatttt tttatattgt attcaatagt ataatccttt tccaaaatat
      781 aacttaccag ttaaaataat ctttattttt acttactctc gccttttaca gaagttaata
      841 gttttttata agttacatca attccaaaag ctccaagagg tgcagttata agtatagcta
      901 gaactgctaa cgtcaaaata agttgtccag atgccatccc cattgcaagt ggtacacctc
      961 ctattgctgc ctgtactgta gcctttggaa tatagccaat catacagaac aacctttcct
     1021 ttattgtcaa atttgtctta ataaggcaga agaatactcc taccatacgg aataaaagaa
     1081 ctccaaatat taaaattaca gctccaattc ctgctttaaa tgcataactt atatctactg
     1141 ttgcccctac caaaacaaac aagagaattt cagcaccaac ccatagtttc gaatacttta
     1201 tagacaatct tcttgcaact acacgatatt ttatctgaag gaaaatccca atactcataa
     1261 ctgctaataa tccagaaaat ggcacaatac cctctaaagc agtttctaaa cttatcatta
     1321 aaaatgagat acttaaaagc aacacccctt tagcagaatc tctcatatga accttcttaa
     1381 aaaatactgc taaacagatt ccaatcactg ctccagctat tacacctaat ataattgata
     1441 ctggaatttg taaaaaacta atagctgata tactatttcc ttgtgccaat cctgtgaata
     1501 ctgtaaacat cacaattacg aaaatatcgt ctatagaagt tcctgctaaa agcatctgtg
     1561 gaatactttt ttctgtacca taaccttctt ccattaattt aagcatttta ggtacaataa
     1621 ttgcgggaga aactgctcct actactgcac ccatgactgc tgcttccaat atagaaacgc
     1681 ctagtaattt tggtgctaaa actatcattc caataatttc aaaagtagct ggaataaagc
     1741 acataagtac agcaggtctt cccactttct tcaaatcgtt tatatctaaa gataatccag
     1801 cccttgttaa tataataatc aatgcaatct ttctaaggtc agaagaaata tctagtattg
     1861 aattatcaat caaatttaaa acatatggac ctaatatgat tcctgtaagc aacattccca
     1921 ataagttagg taattttatc ctcttaaaaa tacctcccga ggccattcct aataaaaaaa
     1981 tcaaagctaa acttgtcaac atattcctac cctatccttt ctctaatatt ctttctcttc
     2041 taatcataca ttatgttgtt ttatttattg ttttgttgaa attttttgta gtgaataaat
     2101 agatttatcc atattaaata tatatgaaat agcgcatact acaaagaaat aaggtaaata
     2161 gctgtaccca aaaacttctg cccctataaa tacaggtgca aaaaaagtat ttgtagcact
     2221 accaaagaca ctagcatagc caagcgctgc aactaattca attggcaaat taaaaaatcc
     2281 tgctaacaat acaccaagac tagctccaat ggaaaacaaa ggtgtaacct ctcctccttg
     2341 aaaacctgct gacaaagtca gtattgtaag tataaacttt aacagccagt caaatgaata
     2401 aatttctcca ccatagaaac tattttgaat taaatttgtc cctaaacctg aatatcttcc
     2461 tttataaaat aaaaggaata atacactaag acatactcca ataatagcaa ttcttatcat
     2521 aggatttttt aatctattcc ctatttttct ttttgacaat ttcagacacc atgcaaatgc
     2581 tccaccaacc attccaaaaa taattcctaa aacaataagc ttccaaaata ttgacaagtc
     2641 aaatacaact ttagaagaaa gtgcaaaaga aaatttctct aaccctaaag cttttgatac
     2701 tgcacttgct gtaaaagaag cagtaaacgc aggaaaaagc gattgatact ccagtgaacc
     2761 agctaccaaa acctccatag caaatagtat tgctgctatt ggtgtctcaa ataatcctgc
     2821 aaaaccagct gccataccag ttactagaaa aatactagaa gcatttttta taggcaacct
     2881 tttacctacc caatgtgaaa aagttgcacc aatctgaaca gcaactcctt ctcgtcctgc
     2941 actaccacca aataaatggg ttaaccatgt acccgatatt ataaatggaa ccaatcttaa
     3001 aggaataatt tcttcttctc catgccctac ctcaaatatt agattcattc ctttactact
     3061 ttttcctcca aatttaaaat agcaataagc aataacaact cctactactg ctaaaaatgg
     3121 tatcagatac attggatatg tttctcgaat atctgtaact tttagaagaa ccgttccaaa
     3181 tatggtgtct ataagaccta taattgcacc aattggtata cctatgagtc ctaacaaaaa
     3241 tagtccacca tatgttttat aaacgctttt aattcttatt atcattttaa tccatattaa
     3301 agttttaaat ttttctatca ttttaattcc tttctatttg catcataaga ttttttgaaa
     3361 gttttattgc atcaactgta gtcaatgtaa tgactatcat aattgttttt taaattgata
     3421 ggatataggt atactcttag cccattctta ataaaataat taacatataa tctactattt
     3481 tttctattaa ttaaatattt cttcaaattt aaaaaataaa aatttagtaa gtacttcacc
     3541 tccattttat cgatttaaac aacaaaaaaa gctggtggca tctatgatat catagaagtc
     3601 atcagctcat tggcagttta ggatattatc atccaaggga gaacctcatt cccataatta
     3661 ttatataatt ttttattttt catatgtcta ttttatgtaa attgtatttt taatgttata
     3721 aataatataa cctatcaatt aaaatattaa agtctctaat attatgagtg ttcaatactt
     3781 catattattt ttatgatata taatttaaat gattttcttt actttgtatt atgatacata
     3841 acttaaatat ttttcctcac tccacattat gatatattat ttaaatattt ttcttcactc
     3901 tgcttatatt atgatacata atttaaa
//
LOCUS       DAELJO010000282         3921 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.284, whole genome shotgun sequence.
ACCESSION   DAELJO010000282 DAELJO010000000
VERSION     DAELJO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.284"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      32..131
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            262..552
                     /locus_tag="KPB88_003666"
     CDS             262..552
                     /locus_tag="KPB88_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG0055900.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     gene            527..1297
                     /locus_tag="KPB88_003667"
     CDS             527..1297
                     /locus_tag="KPB88_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055901.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIESIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            1319..2332
                     /locus_tag="KPB88_003668"
     CDS             1319..2332
                     /locus_tag="KPB88_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0055902.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            2301..3035
                     /locus_tag="KPB88_003669"
     CDS             2301..3035
                     /locus_tag="KPB88_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055903.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            3196..>3921
                     /locus_tag="KPB88_003670"
     CDS             3196..>3921
                     /locus_tag="KPB88_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0055904.1"
                     /translation="MQIDINKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYI"
BASE COUNT         1532 a          427 c          688 g         1274 t
ORIGIN      
        1 ataagtgtag tataatcaag tagaaaagat ataatatatt tcggggagct aatgtgctga
       61 gaggagattt tatctcgacc ctttgaacct gtttggataa tgccagtgta gggagaccag
      121 ttaagataaa aataagtctt atcctatttt ggataagact tttcttttat ttaaatataa
      181 tatcttttta gtaataaaat tttttaataa aaaacaaaat atacctagta ggtattaatt
      241 atttttaaag gagtgattta aatggtaatc gcagacatag cagtaatgcc actaagacct
      301 tacaaaggtg aagaagagat gtataaagta gtagatgctt gtattgctga aattgaaaaa
      361 agtggtctta aatgtgaaat aggagcaatg agcactactg ttgaaggtga ttttgatgaa
      421 gtttttgaat tattaaaaaa ggtacataaa atccctttta atcttggatg tgaaagagtc
      481 ataacagtag ctagagtaga tgaaaaagct ggaggtctga ctatagatga aaaacttaga
      541 aatcatagat aaagtcaaag atagtatata tccaatagca acatttttgt ttatacttat
      601 tctttggcaa tctatggtag gagttttaga agttcctcag tatatactac ccacaccagt
      661 agatataatt aatgtgtttt ttaaagatta tcaaaattta tccatgcatg ctgtggttac
      721 tataggagaa gcaatacttg gttttatagt cgcaattata atatcattgg taattggaat
      781 attgatggat tttgtatcaa taataaaaaa atgtctttat cctattatgt tggttacaca
      841 gatgatacct acaataatta tagcaccact ctttatgata tggtttggat ttggaacaat
      901 gccaaaagta ttgatggtaa tgcttacttg tttttttcct atattaataa gttttgtaga
      961 tggcattgag agtattgata aagattattt aaatttattt aagactatgg actcaaataa
     1021 agttaataca ttcattcacc ttaaatttcc tatggcaatg gataaatttt tttctggact
     1081 taaaatatct gcaacgtatg ctgtaatggc agcaactgtt gcagaatggc taggtggaac
     1141 gaaaggactt ggtgtttata tgcttaggtc aaaaagtgca tatgcacttg ataaagtatt
     1201 tgcatctact atattagtag ttatatttag tttaatgttt gtaggaattg tacaggctgt
     1261 aaaaaaagtt gttataagac atagattaat agattaaagg tatttgaggg aggcaattat
     1321 gaattttaaa aagttaggaa tattaggttt agtaagtgtt tttacattgg caacaataac
     1381 tggatgttct tcaaataaca ataaagacaa aaacaatgat aaagatactt cttctaaaaa
     1441 agtaactatg gtgcttgatt ggacaccaaa tacaaatcat actggattat ttgtagcact
     1501 tgataagggt tactataaag aagaaggttt ggatgttgaa atagtacaac cacctgaaag
     1561 tggagcagaa actttagtag caacaggaaa agctgacttt ggtataagct atcaagaaca
     1621 agttacttat gcaaagacta gtgaagatcc acttccaata aaagctgtag caactgttat
     1681 acaacataat acttctgggt ttgcatcacc aaaagaaaag aatataacaa cagctaaaga
     1741 ttttgaagga aaaacttatg gtggttgggg ctctccatca gaagaagctg tttttaaagc
     1801 agttatgaag aaaaatggag ctgattttaa taagttaaag atagttaata ctggtcaaga
     1861 tgatttcttt gcagctatga aaactgtaga ttttgcatgg atatttgaag gttgggatgc
     1921 agttaaagct gatttaattg gatatgactt aaactttata ccagtaaaag atttagatga
     1981 gagattggat tattatacac cacttataat ttctaatgaa actgtactta aagataatcc
     2041 agagcttgct aaaaaattct taaaagctac aacaaaaggt tatgaatatg caataaaaaa
     2101 tccagaagaa tcagcaaaaa ttttagtaaa acatgcacct gaagtagatg aaaaactagc
     2161 tttaaagagt caagaatatt tagcaagcaa gtacaaagat gatgcaccac gatggggaga
     2221 aatgaaagat agtgtatgga acaactatac atcattctta aaagaatata aattaataga
     2281 taaggatatg aaggcaagtg atgcttacac aaatgaattt ttaccacaat aaaaaagtaa
     2341 aagtatctat agaaaatgta tcaaaaactt ataaagatat tgaagtttta aaagatataa
     2401 gtattgatgt ttatgaaggt gagtttgttt ctatattagg gccaagtgga tgtggaaaaa
     2461 gtactatttt taatattata actaaactta cagattatga tagtggcaaa gtgaatataa
     2521 atggtaagta tagttatatg tatcaaaaag atttactctt gccatataaa actataatag
     2581 ataatgtgtc actaccacta attttaaaaa aagaaaaaaa gagtaaagct aggtcaatgg
     2641 taaaacctta ttttgaggta tttggattaa gcggatatga agataaatat ccttcagaac
     2701 tttctggagg aatgagacag agggctaact tcttgagaac ttttataaat tctaatgaca
     2761 taatgctttt agatgagcct tttggggcac ttgattcatt aactaaaaca tccatgcaaa
     2821 aatggctttt agatgttaag aaaaaagtaa attcaacaat acttcttata actcatgata
     2881 ttgacgaagc tataatgcta tcaaacagaa tttatgttat ttcaaaaaaa ccatcaatag
     2941 taaagaaaga atttgttata gatagtagaa agttaaatga agataacctt gaaaatatca
     3001 ttaaacttaa aaaagaaata atatctttat tgtaaaaaaa aatattttta tttaataagc
     3061 attaaactta gcaatccaaa atgtgagtat acattttgga ttgtttttag ttatatgtca
     3121 aaattttata tattgtgtta ataataatca tctactatga tataatattt aattagaaaa
     3181 gaaggaggtt tgataatgca aatagatata aataaattaa ctcctatgat gaaacagtat
     3241 cttgaagtaa aaaatagata taaagactgt atactgtttt ttagacttgg agatttttat
     3301 gaaatgtttt ttgaagatgc attagtagct tcaaaagctc ttgaaatagc attgacagga
     3361 aaagcttgtg gtttagaaga acgtgctcct atgtgtggtg ttcctttcca ctcagccaat
     3421 tcatatatat caaaattagt tgaaaatgga tataaagtag caataggtga acaaatggaa
     3481 gacccatcta cagcaaaagg aattgttaga agagaggtta taagagtcat cactcctggt
     3541 acagttttag atggaaattt acttgaaaat aaaaagaata attatttatt atctttgtat
     3601 aaggatggta ctaatatagg tctaacttat gttgatataa gtacaggaga aactaatgca
     3661 acatgtttaa atgaagataa ggtaattgaa gaaattgcaa aaatccatcc aactgaaata
     3721 attataaatg atttagactt tatagaaaag cttagagata tagctactgt aagtaatata
     3781 tacataaatg aaagttttag tgataactat ttggatataa atatattaaa agaatatttt
     3841 ccagatgttt atctacaaaa actaaagttt gatgataaag gtctaataaa gtcaagctta
     3901 tcaattcttc tgaattatat a
//
LOCUS       DAELJO010000283         3900 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.285, whole genome shotgun sequence.
ACCESSION   DAELJO010000283 DAELJO010000000
VERSION     DAELJO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.285"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            228..1151
                     /locus_tag="KPB88_003671"
     CDS             228..1151
                     /locus_tag="KPB88_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG0055905.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1126..1953
                     /gene="panB"
                     /locus_tag="KPB88_003672"
     CDS             1126..1953
                     /gene="panB"
                     /locus_tag="KPB88_003672"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG0055906.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1972..2820
                     /locus_tag="KPB88_003673"
     CDS             1972..2820
                     /locus_tag="KPB88_003673"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG0055907.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3231..3413
                     /locus_tag="KPB88_003674"
     CDS             3231..3413
                     /locus_tag="KPB88_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055908.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVIILLCECGLA"
BASE COUNT         1531 a          462 c          567 g         1340 t
ORIGIN      
        1 taaatatcat aattttttta ctgatatttt attatataaa ttttatagaa ttaattgtca
       61 attttttatg aaaatggtaa gatatttatg gatggtatct tgttataaga actatacttt
      121 aaatgttttt aattattttg tcttatgtaa ctcatatcct caggaatgag attagaaatt
      181 aagatgttgt atcacaaagt ggaggtgata ctattaaaat aggctttatt ggttctggaa
      241 aagttggaac atctttggca aattattttt tatcaaaagg ttattgtgtt tcaggctttt
      301 atggaaggaa tcaattatct ttattagaat caacaaattt aacaaaaaca aagatttaca
      361 gcaatttgaa agatataatt tatgaaaacg atatattatt tattacaaca tcagatgatt
      421 ctattgaaat tatagacaaa aaactttcca aatttaattt aaaacataaa tacatttgcc
      481 acacaagtgg ctctttaaaa tctagtatat tgttctgttc aaaaaaagca ggtgcattaa
      541 tttattctat ccatcctata tttgcatttt ccaataaaaa taccaacata aaaaaaatga
      601 aagacatttg tttttccata gaaggtgata atgaacaaga tgatttagtt attcaaaatt
      661 tcatagatga tttgggaaac caatttttta taagagataa ggatacttca tccacttatc
      721 atttagctaa tgttttagtt tcaaatttag tattatcttt actagatata ggagtaagtt
      781 actttattaa attaggttta tctgaagagg aatctttaaa agctatcagt cctttagtaa
      841 aaaaaaatat agaaaatata cttgataatg gctttactaa agctcttact ggtcctgttt
      901 ctcgtggaga tataacacca ataagaaaac atctatctgt attggataaa aaagatgagg
      961 atatttacaa aatactatcc ttaaacctac taaaaataat tgccttagga aatgacaatt
     1021 cattaaaagg tataaaaaat attactatag aaaatgcaat cgaaaattta ataagtaaat
     1081 ctgaaaaata caaagaaatt tacaaacttt taggaggaaa aaacgatgaa aaataccata
     1141 cagactttta agaatgctaa atatgaagga aaaaaactat ctatgcttac tgcttatgac
     1201 tactcaattg caaaaataat ggatgagtgt gatataaatg gtattttaat aggagattca
     1261 ttaggaatgg tcataaaagg tgaagaaaac actctatctg ttaccataga cgaaattata
     1321 tatcatacaa aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag tgatatgcct
     1381 tttctatctt accatgtttc tatagaagat gcagttaaaa acgctggtag acttataaaa
     1441 gaaggaggag ctcatgctgt aaagttagaa ggtggctcaa acgttataaa gcaaatagaa
     1501 agcattgtaa atgctcaaat acctgtaatg gggcatttag gattaactcc tcaatcagta
     1561 aattcatttg gtgggtttaa ggttcaagga aataccagtg aaacagcaag acaacttatt
     1621 gaagatgcta aattaataga aaaagcaggt gcattctcta ttgtccttga aggagtgcct
     1681 acgaaaatag ctgagatggt aactaattct atttcaattc ctacaatagg tataggggca
     1741 ggaattaact gtgatggaca aattttagtt tatcaagata tgttgggaat gtttggagat
     1801 tttgttccta agtttgtaaa gcagtatgca aatataggag atataatgaa agattcaatt
     1861 aaaaattaca tcctagaagt taatacaggt acatttccac aagaaaaaca cagcttttca
     1921 ataaatgaat ctgagttaga aaaattatac taatattaag gatggtacaa aatgcttgta
     1981 aaagaaatta aattactaag aaatataatc aaagattgga gaaaacatgg ctattctata
     2041 gggcttgtta caacaatggg atttttacat gaaggacacc aaagtcttat aaaaaaagct
     2101 gtaaaagaaa atgataaagt agtagtgagt gtatttgtaa atccaactca atttggacca
     2161 aatgaagatt ttaatagcta tccaagagat atagataaag attttaaata ttgtatggat
     2221 agtggagcaa cagtagtctt taacccatca ccagaagaaa tgtatttaaa aggtaattgt
     2281 actaccatta atgtttctgg acttacagat tttctttgtg gagctaaaag acctgttcat
     2341 tttggaggtg tctgcctagt tgtttcaaag tttctaaata tagtaacacc tgataaagct
     2401 tattttggag agaaggatgc ccaacaatta gctgtaatta aaagaatggt caaggattta
     2461 aacatagata cagaaataat aggctgtcct ataattcgtg aaaatgatgg attagcaaaa
     2521 agttcgagaa atacatatct ctctgaggaa gaacgaaaat ctgctctaat attaaataag
     2581 agcttaagtt tagcaaaaga agaattagta aaaggcaatc taaatccaga aaatataaaa
     2641 gaattaataa cagctaaaat aaattctgaa cacttagcaa aaatagacta tgttgaaatt
     2701 gtagatagtg agacattaca acctgttaag caaatagaac attctatact tgtggcaatt
     2761 gctgtgttta taggaaaaac cagactaatt gataatttta catttgagtt aaatatatag
     2821 tctataattt tatataatat atctaaaaaa taatagaata cctaaaaagt aataaattac
     2881 ctaaaaaata ataaattgct tagaaaataa tagattacaa taactaaaat tttaaaatta
     2941 aattccttaa aagtatgatt aattataatt atataagtat attcttatta cacttatatt
     3001 aatttatatt aaatatctac taactcaaat attcataatt aaactttaaa ctggataaat
     3061 tttcttttaa gaatgaaagt ttatccagtt ttgcgattat aataaccagt tttacatatc
     3121 cacttgtaaa atatatctaa gcaatatatc atactatata gatttatgag tgaaataata
     3181 tctgctattt actgaatatt tttatttttc gttttttagg gggattagtt gtgtttaaag
     3241 ataagattga tgaatgtgtt catataatga ctgcatatat tgctagttta aaagaatact
     3301 attcattcat agaaactcaa attggtgatt ttattaaaaa atatggagaa gatgttgtag
     3361 agttatgtct ccatagagtg ataattctac tatgcgaatg tggtttggca taaaattaaa
     3421 tttattaaaa tgggtaagaa gaatcctata taatattctt cttacccatt ttaatatatc
     3481 aatttcatag cttatcgttt aaaatatttt acatccatta ctagacaact ttacattctc
     3541 tttttaattc tagtatattt aaatcatttt agttgctcct tatttattct tattacttgt
     3601 tttattttat aaataaaata attcttttta ataatgtttt ttgttgatat tcctatcaca
     3661 catccaatta tttatcattg tcttgaaatt gtttgcatac ttaatttaat ccaattgaaa
     3721 caccccagca tagaatcccc tgtaaaatag aagttggatt aaatcctaac atctatacag
     3781 aaaatactac tccaagcaca agcaatataa tggaatatat ttatcactta attttttata
     3841 ctttttaaat cccataccta aaacattaag tgcagctatt agaattaata attgttgtgg
//
LOCUS       DAELJO010000284         3896 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.286, whole genome shotgun sequence.
ACCESSION   DAELJO010000284 DAELJO010000000
VERSION     DAELJO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.286"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(7..1026)
                     /locus_tag="KPB88_003675"
     CDS             complement(7..1026)
                     /locus_tag="KPB88_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0055909.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1202..1741)
                     /locus_tag="KPB88_003676"
     CDS             complement(1202..1741)
                     /locus_tag="KPB88_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0055910.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1842..2522)
                     /locus_tag="KPB88_003677"
     CDS             complement(1842..2522)
                     /locus_tag="KPB88_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG0055911.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILETLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2551..3840)
                     /locus_tag="KPB88_003678"
     CDS             complement(2551..3840)
                     /locus_tag="KPB88_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0055912.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1293 a          711 c          368 g         1524 t
ORIGIN      
        1 tataaactat atactatcag ctggataaac aatactactt tgtcttctta tctcatccat
       61 aatttccatt actattattg aattttcatg agaatttata tttgattcta ttttattgtt
      121 atttataaga cttataaact ctacaagttc ataatacatt ccttctattt ctctaggctt
      181 ttcctctgct ttttcttttg gaatagatat atcttcttct cttccatctc tgtatttaat
      241 aatagctttt tcaaatagat taagcttttc aattattata cttccctctt ctccctgtat
      301 ttcagatggt atataagaat cagctacttt agaatactga attatcgcat ccatatcatc
      361 atattgaaat acagctgaac cttctccatc cactccagtt tgaagtatat gagaaactgc
      421 ctttacagtt tttggagcac caaataaatt caccatagga tgtatacaat acactcctat
      481 atccattaaa gctccatttg acaactcttt tttaaaggca ttttctataa ttccattttt
      541 atatttatca tatcttgaag aatactgaca atagcttcca aaaaatcttc ttattttccc
      601 tattttatac aagttttctt taactgcttt aaagtttgga acacatgtaa ttctcatagc
      661 ttccataagc aatacattat tatctttggc tgtttttatc attgatttga cttcttttaa
      721 attagatgct aacgactttt cacaaagcac atgttttttg tgttttaaac aagtaatggt
      781 ttgctttgaa tgtaatgcat ttggagaagc tatgtataca gcatctatat ccttcgattt
      841 agccatttgt tctaaatcat caaaaaaaat acttgctcca tatagagttc caaattctct
      901 agctttttct aaatttctag aatatacagc aaccaattca aagtctttaa ccctagatgc
      961 agctcttaga aatatatttg ctatattgct tgtcccaaca ataccaaatc ttattttacc
     1021 catcataaat cactccccat tttctacact ttattttaat tttaataaaa aactttacaa
     1081 cttatagata tattataaaa taattttatc aaataaaaaa gaaagctaat tttaaatgat
     1141 ttcaccattt taacatagct ttcttttaac tacaatctaa attcaactta tatttttaaa
     1201 gttatctttc tactatatct ttatttcccc tcaaaaattt tatagtaaga atttcatcca
     1261 caaacaatgt tactaaaagt atagttacaa caattggcca ataaggtatt ataagactat
     1321 ataaactatc aactatatac acttgaaaat atctaactcc aataaatgtc aatatagtcc
     1381 aatatacaga aaactttaga catactattc ctctactatt gtacttataa tctgaataat
     1441 cccaatagac cttattaaaa tatactttca taagtattcc tgtaatatat tctactactg
     1501 tcggcactac tagacataat aaaagcaatg caaatatatt attggggtca ataaatcttg
     1561 agacctctat tataatcgac attgcaattg catacattgg tttaaatgga ccatttaaaa
     1621 atccatcttc ttgaaaatgc ccttgtttat aataactata taagttttca attatccaac
     1681 ctataaaacc atacaagaaa aaattaaata aaaaatataa tagagaacct acattatcca
     1741 tgaatattcc tcctagctgt ttatacttat tttttctcat attcatggat ttattctaaa
     1801 tcaaagaaat tatatttaat ttatttaatt aatactttat attatagatt gtgacattct
     1861 ttagagttca aactttttag gtattctcta acttccagta aagtttcaag tatcttatgt
     1921 gattgtgatt taatttgctc atctggctct tttaaatctg gtacatttat acatcttata
     1981 ccaccattgt atgcagcttc gactcccata ggcgaatcct ctatgacaat acaatttttg
     2041 ggattaacat ttatcttttt agctgctttt aggaatatct cagggtttgg tttagagttt
     2101 acaacatcat ctccacaaac tatagcatca aagtaatctt ttaaatttgc cttagctaat
     2161 ctctttactg ctctttctct ctttgttgat gttgcaacag ccattttgta tccattttct
     2221 tttaaaaaag agattagttc attgactcct aatttgatag gagctccctt cctctccata
     2281 aattcaatca tatttttagt tttttcgtca tataaatcta ttattggtac agaactatcg
     2341 tatatatctg taagaccttc tattattcct ttacgatttc ttcccataac tgatgtataa
     2401 atttccttag tcatagtata tccatacttt tcaaacgtct ccatccaaaa ttcaagagaa
     2461 attctctctg agtcaaacaa aactccatcc atgtcaaaaa taattccttc tactttttgc
     2521 atacaattcc tccaatattt tttgatataa ttatattaca ctttttatga ttaattttac
     2581 aaccctcaat ttatccaatt ctaatattgt aaaattgtaa ttttcaaaag ttacactttg
     2641 gtttacttta atatttgaac caagcatata ggatatccat ccacctatac tgtcaaatcc
     2701 atcatgctct atatcaagtc caaaatactt attaatatag ttgatagatt cagttccatc
     2761 aactaagtaa gaacctgctc ctattttctt aatatgagtc tcttctttgt caaattcatc
     2821 ttgaatttct ccaactatct cttctagtat atcttctatt gttaccacac cagatgtacc
     2881 accatattca tccacaacta tagcaagttg aagtttttcc tttcttattc tctcaagagc
     2941 tttatctata gttaaatttt cagatatata tataatatct ctcaatattt cttctagttc
     3001 aattttattt tcatttatct tttgattgta taaatctctt atatgtacaa aacctaatat
     3061 atcatcttta ttttttctgc atactggata tcttgtaaca ccttcctctt ttaaaatagc
     3121 taatatcttt tcttcagaat cactttcata tatacatacc atatcagttc taggaaccat
     3181 tatctctcta attttctttt cttcaaattc aaaaatatta tcaactaatc tttgctctga
     3241 ttcatctata agtccacttt tataactttc ttctactaat agtcttattt cgtcacctgt
     3301 atggggttca tcaacctcat cagcttgtga atatccaaat ggtttaagaa ataaatcagt
     3361 acttaagttg aaaaaatata ttattggata tgttaactta taaaatccta ctaataaaaa
     3421 agatgtactc aacattattt tttctgtatt ataaagtgct aaagctttag gaaccaactc
     3481 tcctattaca acctccatca ttgttataat taaaaaagaa atggaaattg atatagtata
     3541 tataatactt tcactaagat taaatacact tactattggt aaaattaact ctttaactgt
     3601 agattctccc atccaaccta atgcaagtga acaaagtgtt ataccaagtt gacatgcaga
     3661 aagataggaa tttaagttgt cttttattat tttacatctt tttgcattat tattaccttc
     3721 agttatcatg gtatcaatcc tagaactacg aagttttacc atagcaaatt cacaagctac
     3781 aaaaaaagca tttatcaaaa caagcattac tataagtgtt acttttaata actcccgcat
     3841 aaagtattgg ttcttataat gtaagaaatc tataagaact caaagttccc ccctta
//
LOCUS       DAELJO010000285         3893 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.287, whole genome shotgun sequence.
ACCESSION   DAELJO010000285 DAELJO010000000
VERSION     DAELJO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.287"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(227..1045)
                     /locus_tag="KPB88_003679"
     CDS             complement(227..1045)
                     /locus_tag="KPB88_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0055913.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1213..1794
                     /locus_tag="KPB88_003680"
     CDS             1213..1794
                     /locus_tag="KPB88_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0055914.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1791..1985
                     /locus_tag="KPB88_003681"
     CDS             1791..1985
                     /locus_tag="KPB88_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055915.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2149..3891)
                     /locus_tag="KPB88_003682"
     CDS             complement(2149..3891)
                     /locus_tag="KPB88_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG0055916.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNKVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         1370 a          528 c          510 g         1485 t
ORIGIN      
        1 agataatatc ccaatcaaat tctagtcatt taaacataac atcacaagtg atttatctga
       61 caaattaaga taatatccta attaaattgt agatatttat acataacact aaaagtaatt
      121 tgtttaacaa attaagataa tatcctaatc aaattgtagg tatttataca taacactaaa
      181 agtaatttgt ctaacaaatt aaaatattat ccaattaata taaaaattac ttctttttaa
      241 ctgggaagca tacctctgtt acccaattat caggattact atcgttgcca gggcttacat
      301 gataaatatt aaacatagga ccatccacat catagttatt atctgatatc caattaccaa
      361 tagcctcaca agcgtctgca acttgattga aatttccatt aacaattgca gttgcagcag
      421 tcacacttgg aacagtctta aatttaacat gttcagtatc tttatacttt ccagatacag
      481 caacttgtac ttcaacatct acataatctt ctttatatcc agtatcataa aaaactgctt
      541 tagaatactt tgggggttca atttgaacat tctggtcttt tgtctctaaa aatgcttgat
      601 gccataacat tccttcgtct tgataagttg gtataaccct tctaagtgtc atcatatatt
      661 ttggtgcaaa attctttata gttacatcat aatttttcat aatatcattt cctccaattc
      721 ttttaatagt ggtttctatt tttaatagtt ttttctgtgt atcttctaat tgttctttta
      781 cttgagaatg gtggatattt agatatttta tgagactttc tttatcgtta tattctgtca
      841 aaatttcttt tatagaatac aagccaaagc ccatatcttt tagtgcatgg attctgtttg
      901 tagtagaaag ttggtctgca ctatagtatc tatatccatt agttttattc gtatgagaag
      961 gagttaaaag tcctatttca tcataatgac gtaacatacg aatactaatt ttacttagct
     1021 ttgaaaaatc gcctatctta aacatgtttt tgtctccttt ataaaggtac ttttagctta
     1081 atattaatat aaaccatatc ataatgttag agtcaactaa aattataatt aaattatatt
     1141 gacatatgat gaaaaaaata ttatactgaa catgttcaga accaaaaatg ttaattgttt
     1201 tggaggtcta ttatgaaaag taatgataaa gatgctagag aacgtataat tgaagttaca
     1261 ttgaatcttt taaatgaagt agatgatatt gaggagatta cagttagaaa aattgctgag
     1321 cgtgcaaatg ttggagttgg tttaataaat tatcatttta aaacaaagga taatttgttg
     1381 agtactgcca taggagatgt gatgtcaaat ataatagcag agttatatga cgatagtgta
     1441 tatactctaa gacctattga agatttgaaa aatttattaa aaaaattgtg tgatactgga
     1501 ttacattatg aaaaagtgct tccttttgtg ttgaatcaat gtataaccaa tggagatatg
     1561 caagcagaac ttgatattgt ccctatgctt cgtaaaattt ttggaaataa aaaagatgaa
     1621 atgagtctaa gaatcattgc cctgcaaatt attttaccaa tacagatttc tgctttatct
     1681 acagaatcat ttcagttata cagtggtata aatataaaga ataaatatga gcgagataaa
     1741 ttcattgata ttttaataga aaatattatt ggagaggatg ttgatgtaag atgaaaatta
     1801 taactattgg ttcaagtctt atcactgttt tactttttct ttcaactatg gtttgtgggt
     1861 tctggattaa gaataataag gtaacagatg caagttctat taagttccat atgaatagtg
     1921 ctatttttac agggatattt ttattgattt ccacaatcct tttaattata tatataaaaa
     1981 aataataata ttattaataa ataaagtatc agaaattata aagacaagag tcttaaaaat
     2041 ttctgatact ttaccatata attttattaa acattaattg tgtgtgaaaa aatatcactt
     2101 atttcaatct aatactaaat cttaagagtg agtataaagt attagaaatt aagaagcatt
     2161 ttttagttca agtggattat ctataccaaa ataatcatat aaagtagagc ttatttcctc
     2221 taaatttcca tcttggaaag gtaattgtaa atttaccttt tctaccacat ctttaaatcc
     2281 agcatcagta ttttgattta aaaaatccat atatacatca acagcttttc tgtaatcttc
     2341 ttgtgataat tgccatatct gaagtgatac atcagatgca agaccataac tcaagtaata
     2401 aaaaggaact tggaagttat gagatacaag tatccaaaaa ggagcatttt tttctctaag
     2461 tatattattg gaaactccat attcttcctc taaatcaaaa aacaatttat tgatatcatt
     2521 tagactcata tatggattct tgtatataat ttcttgaaat tcatcataaa gacaagcatc
     2581 aataatagta tttaaaacaa tcgataagtg ttcttttttt atagcttcag attgtttacc
     2641 aaaaaaatca tcaaaatatt tataaaatag aatttctaaa gatgttgaat gaacttcaca
     2701 tacatctata cttggttgca tcttattatt taagttaata ctgttgtagt aattataaaa
     2761 atgaccaaac tcatgagcaa aactagtaac acctaaaaaa gaattgtccc atgtattaaa
     2821 caagaaaggt gctttatatt tatttatata agtagtatat cctccaggtg atttgttttt
     2881 tccagaagaa taatcgtaca agtcatattt tttcatatag tcgaagctct cttttagttt
     2941 tggagatata tcttcaagta ctttatcaaa cttcctaaat gagcgatttt tatatacttt
     3001 aatataaata gaagaatcag atggtttaga tgaaatctct ctatataatg gaactatgta
     3061 atcttttaca tcttgtctaa attttttagc ttcttcttga gaatagtctt tgtttaggtt
     3121 catataggca taatctatat aattcttaaa tccttgctta cttgctattt cagttcttgt
     3181 ctgtataagt tctaagaata tgttaccaaa tataggattg tattttttta agtaatctga
     3241 ataaattttc acatattctt cagggctaag atttggtctt gacaaggctt cttcaaagtc
     3301 tacctctata ccatctattg atactgtaga tttagctaat aagtcttcat attttacaac
     3361 taggtcttgc tcttttttaa gcaaaccttc aacttcttta gaattaagtt ttccaatgtt
     3421 ctttagataa ttaaatgtat cctttccaag ttctttttct aattctttag agaatttaga
     3481 agttacaaat ctatcttgaa agtctttgta tgacatgtca acatctactg atttactcat
     3541 aagatactta tattccttag agtaaaattc gtctgatgaa tctatgttat ttctaatttt
     3601 tgctatagta agtgtactgt aaaaatcttg ataaatttta tcaactttat taaataaatt
     3661 taattgttcc ttagctgttt tagctgttaa aagtttgtcg ttacacttgt taatgttttc
     3721 gcaaattgac tgtatattag gacgcttata ttccattttt tcaaaaggaa tagttcctct
     3781 aacagaatct ttttttgtat cattagtata atttcttatg tatgatgcaa atataataaa
     3841 aactgcaatt aaaattagaa aaacaaatat tttcttcttg gatttaacca taa
//
LOCUS       DAELJO010000286         3886 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.288, whole genome shotgun sequence.
ACCESSION   DAELJO010000286 DAELJO010000000
VERSION     DAELJO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.288"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(216..1466)
                     /locus_tag="KPB88_003683"
     CDS             complement(216..1466)
                     /locus_tag="KPB88_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG0055917.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1498..2439)
                     /locus_tag="KPB88_003684"
     CDS             complement(1498..2439)
                     /locus_tag="KPB88_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG0055918.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2679..3755)
                     /locus_tag="KPB88_003685"
     CDS             complement(2679..3755)
                     /locus_tag="KPB88_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0055919.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1164 a          714 c          417 g         1591 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaacga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta
     1501 taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat
     1561 ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga
     1621 aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat
     1681 agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa
     1741 accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc
     1801 agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt
     1861 ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg cctcacccat
     1921 tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc
     1981 aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt
     2041 tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag
     2101 aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt
     2161 cataactttt atgccattag cttgagcata tttaacatct atattatcca gacctactcc
     2221 acctcttact atcaacttca actttttacc ctctaatgct ttatctatta catctttagt
     2281 aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac
     2341 atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc
     2401 ttctttctct attccatcag taactaatat attatacata acaaactcct ccaattttta
     2461 ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact
     2521 taatttaact ttctgtttat ataattttca aaatattttt ttattaaagt catatcaaat
     2581 ttttctattg tttgttaaaa atttaatatg taatttttat aaaatattat ttattatata
     2641 ttctaaattt atcttaaaac atacatattt ttacaatatt atttattaaa acctaatata
     2701 tcatcaatat tatctaataa ttcttgtaca tcttcgacag tatagtcacc catatgtgaa
     2761 attctaaaag ttttttcttt taaatctcca tagccatttg ctatttggaa tcctcgttct
     2821 tgtaattttg aatttaaatt tgatacacta ataccttgag tattttctat aacagtaagt
     2881 gtattggata agtgattttc attggtaaaa atttggaagt gttttttagc ccagtttcta
     2941 actaagtttg ccatgtcttc atgtctgttg aatctgttat ctaggccttc atctaatata
     3001 ttatctaatt ggaaatctaa agcgaacatg tgagaaagag atggtgttga agggtattgg
     3061 tagtttttct tgattaagta ttcatacatt gctaacaaat caagatatac tcctctaaaa
     3121 ggtacttgct tagctctatc gattgctttt tgagaaaatg tgcaaataga cattcctggt
     3181 ggtaatccca gtgctttttg tactgatgtt atacaaatat ctattccaat cttatcaacc
     3241 tcgactttta ttccaccggc tgaacttaca gtatctacac agtagattac atcctcatac
     3301 tttttaacaa cttctccaat ttcttctatt ggatttctta ttccagttga agtttcattg
     3361 tgagttacag ttattaaatc gtattctccg gtagaaagca ccttatcaac catttctggc
     3421 gttgttgcct ctcctaattc aactttaaat atatccgctg ggacattatt agccactgcc
     3481 attttgtacc atctatcacc aaaggatcca caggaaaata ctgctgcctt tttggatgta
     3541 caggaacgaa tggagccttc cattaaacca gttccggatg acgtggataa aagtatcgtg
     3601 ttgtttgtat agaatagttt ttgcatcttt tcacttatac tcttttggag catagatgca
     3661 tcttttcctc tatgtcctat cattggtgta gccatctttt gcaagacttc ttctttaaca
     3721 tcaataggac caggaataaa taatttctta ctcattataa ttcccccctt tttagtttgt
     3781 aatatggata gcgtttttac tgtccttatt tttaataact ataatatcaa gctgaattaa
     3841 ctatgtcaac ataaaattta ttttttatat ataatttatt ttatat
//
LOCUS       DAELJO010000287         3879 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.289, whole genome shotgun sequence.
ACCESSION   DAELJO010000287 DAELJO010000000
VERSION     DAELJO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.289"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..558)
                     /locus_tag="KPB88_003686"
     CDS             complement(<1..558)
                     /locus_tag="KPB88_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0055920.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFEDDKFNIQMSKNEINIQETKTIKIGAIIG
                     LSGVNDEYHLRVLDKDAEKEEANSKF"
     gene            complement(570..779)
                     /locus_tag="KPB88_003687"
     CDS             complement(570..779)
                     /locus_tag="KPB88_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055921.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKEALRKKAKALAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFKNL"
     gene            complement(859..1932)
                     /locus_tag="KPB88_003688"
     CDS             complement(859..1932)
                     /locus_tag="KPB88_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG0055922.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            complement(2040..2375)
                     /locus_tag="KPB88_003689"
     CDS             complement(2040..2375)
                     /locus_tag="KPB88_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055923.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
     gene            complement(2493..2858)
                     /locus_tag="KPB88_003690"
     CDS             complement(2493..2858)
                     /locus_tag="KPB88_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055924.1"
                     /translation="MLKVEKYFSGSLGTNIIDDDPTCRNYLALYCCVHGVRKNGELIF
                     PTCEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            complement(2872..3129)
                     /locus_tag="KPB88_003691"
     CDS             complement(2872..3129)
                     /locus_tag="KPB88_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055925.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKECVYAMFGDMDNFKKIGEYEDE
                     KRAIQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            complement(3463..3870)
                     /locus_tag="KPB88_003692"
     CDS             complement(3463..3870)
                     /locus_tag="KPB88_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0055926.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
BASE COUNT         1136 a          784 c          377 g         1582 t
ORIGIN      
        1 aaacttagaa ttagcttctt ccttttctgc atccttgtct aagactctta aatgatattc
       61 atcattgact ccactcaaac cgataattgc tcctatttta attgtcttag tctcttgtat
      121 gttaatttca tttttagaca tctgtatatt aaatttatca tcttcaaact caattgaatg
      181 agtatatgac ttattgtaat caagttttaa tatagcaact ttcttttcat ctttttgaga
      241 gtataagcaa attgctaagt cgcaagattc taatgtagca ttcaatttca taacatcaaa
      301 taaataagct gcaatctctt tagagttatt taaaaatgaa ctttcatcat aaataatttg
      361 ttcacaacac ttcttaatta gattgttact atagtcatta aatactgctg ttctgatgtc
      421 attatctctt gatactttgc ttattttctt ttgaaaaaat aggtccatat cttgactaac
      481 tctaccttca aaatcattta gtattggtgt atcgctgttc ttatctaaaa catgtattat
      541 aaatttgtgt attatcataa tttcacccct tataaatttt taaagcgttc tataatcttc
      601 tcgcttatag tattttttat aacttcgtct accttatcta tagttattag tactatattt
      661 tcatctttag ccaatgcctt tgctttcttt cttaaagctt ctttacttcc atatgtataa
      721 tgtatttttc tattttctaa cgataatcct atttgccatc ttaatatata ttcatacatt
      781 tatcccaccc cttattttca tttttgagag ttacaaaaca cttcaaaaat attcatacta
      841 aaagacattt tgcaactttt agcccattct ttttgctatt tcatatacaa catttgcagt
      901 aacagcattt cctgcttgct tgtacagttg actatctgag catacacttg ctgctctttc
      961 gtaatattta tccggaaatc cttgcaacct aaagcattcc tttggtgtta accttcttat
     1021 atctccattt ttcaaaattc catgtttatc ttgagctgtc aatgtgaaca ttgtttctcc
     1081 gctttcttta attctacgac cattttgtct tttatttacc ctatcgggcg ttaaaactgc
     1141 attaactaaa actccactat tatcacaatt tctatttgtc acacctgcat tatattttgc
     1201 tttaaggcat ctagcattta ttgttacttt agagttttta tttaagtcta taaagtatag
     1261 acctgtttta gcacctccac ctcctgcctg acttctaata catctagcaa ttccaactgc
     1321 atcataaatt ctatttgtac tatgagttgg attatttagt tgctcaagat tttttctact
     1381 ttttctttcg ataggaaata cttttcgtgt acttcgtcct ctaaaatgtc caacaatgaa
     1441 tattcgttct ctattttggg gtactccgaa gtttttagaa ttaagaactt gccactctgc
     1501 atcatagccg atttcatcca gttcaacgag aactttgagg aaatcaaatc ctccattaac
     1561 actaagtaga tttttaacgt tttcaataag taaatacttg ggtctatctt cttctttgag
     1621 ttctctaata agttttgtaa ctgtaaaaaa taaacttgaa cgttctcctc tgaatccaaa
     1681 ttgtttccct gcaacagaaa tgtcttgaca tgggaatcca aaacaccaga catctgctct
     1741 tgggatattt tctgttctaa tttctctaat atctctttca aaccattcat cctccttcgg
     1801 tttgtgcatg gcattataac ttaaatttgc gaatttatca tattcgcaat gtcccaaaca
     1861 tttatgtcct gctttttcca tccctagcct aaagccacct atccctgcga ataaatctaa
     1921 aaatgtaagc aatacaacgc ctccttattt tcatttttga gagttacaaa acatctcaat
     1981 gataatttta ttaaaaaaca ttttgcaact ctctaaactg ttttaattag atatttcttc
     2041 tattcaaata taagttcttc gctatcaagc cactttttaa taccatcttc acaatcatat
     2101 tcaatatcat caatcttaca gtcataaata caacattcgc atatcttttt atcatgtaaa
     2161 aaatctatta atctattgat gaatagtaac tctttctctt gtaacttttc tttaagactt
     2221 ttattttctt ctcttaacac actaatttca ttaaaaacat ctaaaagtac ttttgaatca
     2281 gcttcatcat tttcatttaa attcaatcta tactcataaa ctctaccagc tataaaactt
     2341 cctattacta atatcacact agctaagata ttcactttta accatctcct catattcttc
     2401 tctagcctta tctatagcaa taaatatatc ctctccatta tcatataact cttttgctct
     2461 tttaattgtg tattcagtcc ttgaaacttc cattattcct cctcaatata ttcagctttc
     2521 cagccacttc ttgttttagt tttcttttta attgtttggt aaactgcctg actctgtagt
     2581 cttaaaaaac atgctgcact atctatagaa tcaaatattt tttcttcacc agttttggca
     2641 ttaatcaact ttacctttga acctttcttt ttcttttttc tatttttatc aacattaaac
     2701 tctattaaca ttttttcaca tgttggaaat ataagttctc cattttttct tactccgtga
     2761 acacaacaat atagtgctaa gtaatttcta catgtagggt catcatctat gatatttgtt
     2821 cctaaagaac cactaaaata tttttcaacc tttaacattt cagtaacctc cctatttaac
     2881 tggcatttga aatattctat ttctataact tctaacctta taactgtcta tagaatctgt
     2941 tctagttcca ccttcaataa atctttgtat attatccagc acttgtatag cccttttttc
     3001 atcctcatac tcacctatct ttttaaagtt atccatatct ccaaacattg catatacaca
     3061 ttctttatca acatttatcc aatcagcttt tactaaatca gttttatctt gacttctaat
     3121 tattatcatt cctaataccc ccatcatcat tttttctgct ataacttcta ataaatacaa
     3181 tattttaagc cacagcattt cttcaaaaag cagtttacct agatatagcg tcactgtagc
     3241 tgttccttct gcctttaaat tccaaggtct atgcgaatta tctgttttta tattatttaa
     3301 gcttattttt attgctcctt cgcaattttg taccatctca attttctcac cattacgctc
     3361 taaaaaaaat tttctatact cttcttccat tttccatccc tccaatattt taactcctag
     3421 gaagtaatat tgcataatta ctccctagat tatttaactt aattaaaaag gtatatcgtc
     3481 atcatctatt gcttgaaaac cttgtgggtc taatcctggt ggtacatatt cttgtttagc
     3541 attattatca tttttactag aaagtagttc taaagcattt acattaacct tagtaataga
     3601 tttccagcaa ccattttcat ctttgtaatt atatatattt aactctccaa cagcatatat
     3661 aggcttacct ttaacaagat attgcactaa attctctaca tgttttccta attgctcgca
     3721 ttgaataaaa tcagttattt tatttccatt tttatcttta aaccttctat ctactgccat
     3781 tgaaaaggtt atttttggag tacctgaatt tggaaggtac ttcaattctg catctgcaac
     3841 taatcttcca actaaagtta ttgtattcat ttaactagc
//
LOCUS       DAELJO010000288         3872 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.290, whole genome shotgun sequence.
ACCESSION   DAELJO010000288 DAELJO010000000
VERSION     DAELJO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.290"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..595
                     /locus_tag="KPB88_003693"
     CDS             <1..595
                     /locus_tag="KPB88_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG0055927.1"
                     /translation="IIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYMFNL
                     ININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFG
                     TGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKAL
                     FNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            549..977
                     /gene="ytfJ"
                     /locus_tag="KPB88_003694"
     CDS             549..977
                     /gene="ytfJ"
                     /locus_tag="KPB88_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG0055928.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1225..1665
                     /locus_tag="KPB88_003695"
     CDS             1225..1665
                     /locus_tag="KPB88_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0055929.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1681..3132
                     /locus_tag="KPB88_003696"
     CDS             1681..3132
                     /locus_tag="KPB88_003696"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG0055930.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDIITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIIKNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKAMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3223..>3872)
                     /locus_tag="KPB88_003697"
     CDS             complement(3223..>3872)
                     /locus_tag="KPB88_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG0055931.1"
                     /translation="SSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIICTLLVIIM
                     ALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGEL
                     IFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSF
                     IFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
BASE COUNT         1615 a          350 c          670 g         1237 t
ORIGIN      
        1 aataatagct gtaattttta ttataagtgt aactatgttg attttaatat ctcatataaa
       61 catcttaata atagctgata taaaaaacaa agatatttgt ttaaagctta atataaaata
      121 catgtttaat ttaataaata taaatagaca actttatcca gctgaaaata gtaaaaataa
      181 tgataaaaaa gaggggatga agaataatat agattcaagt atattattag ctgatgacct
      241 tttgagcatt tatagattat taaaaaagat aaaaatacat gaactttatt caaatattaa
      301 ttttggaact ggaaatattg gcttaacatc ttctgtatat gtattgataa atactttgta
      361 tggaaattta tttaatatga ttgatgcaga aaaaatgtat ttaaatgtaa atccagattt
      421 tacaaaagat tatgttttag gaaatataag gatacatata aggccaagaa taaaagcttt
      481 atttaatatt ataataatga ttaataaaat tatgaataaa aataaaggaa ataaggaagg
      541 tgatagtaat gagagcaaca ggttcgatac agagtcttat ggaaacaact cttgaaacaa
      601 ttaaaggttc tatagatgca aatactatta taggagaccc tataaaaaca gatactacag
      661 tggttgtacc tatatcaaag gttacaattg gctttggtat aggaggagga gaatactcaa
      721 aaggatatga tgataaagat agggaaattg aattaaaaaa tgaaaaatca gatacgaatt
      781 ttgctggagg tagcgcaggg gctatatctg tacaaccagt agcatttgta gtagttgaaa
      841 gtggagagac tagaataatg agtttagata gtaatatcaa tctagtggat aatatattat
      901 ctataactcc tagagtattg gagaaaattc aaaatatatc acaaagtaat aagaatcaag
      961 ataaaaacaa tatgtaagaa taaaatattt ttaaatatgt atatagtttt aagtaaagta
     1021 gtggggttaa tataaatccc actattttgt tgtgtacttt aaacatttat atctaataag
     1081 attttagttt aaaacaattt aaatagataa taaagttgat atatagtata attataaaaa
     1141 gaaaagaagt attatattat agatatataa acgcatttga gatataagag ggagttataa
     1201 atcattagtt ttgaggtgaa tgttatgagc gataaagaat tatctatttt gttgttagaa
     1261 agtatgaata attttcataa tttaataaag attattaata acgaaagata taaaaaagat
     1321 aaggttctta cagaaagaca gttctttgcc ttagtaaaaa taagaaaaca tgataaaata
     1381 gagcttaaaa atctaagtag agatttgcat gtatctactt ctagtctgtg tatactactt
     1441 aataaattgg ttgagcaaga gtatgtatat agggaagaag atagtagaga tagacgaaat
     1501 actttttatg gaataactaa aaatggagaa aagatattgg ataatgaaat actaaagttt
     1561 gtatctataa taagtgataa aatggattgc ctagatatag ataataaaga taaattattt
     1621 acttctttgg aagagtctaa aaatataata gaacaattat tttaaaaagg agaatttatt
     1681 atgaatataa agtattctaa agagatatta aaaagtttag aaaatgctat aacagtagta
     1741 gctctatttg agggagaaga tgtttttacg acatttgata aaataaatat gataagcaga
     1801 aattttaatt ctaaagaaaa tagtatagat actgtaacat tggttgaaaa taacaatttg
     1861 gtaaatgttt gtcttgttgg ttttggtaag agagagttat tgaatagaga aaaaattaga
     1921 atcataggtg ggaatttgtg taaaaaatta aaaaatatat tgaaaaatga agcatataag
     1981 agtagtgatg ttaatgtact gaatcttaat ttaaatagtg atgaaatagg ttgttttgta
     2041 gaagggatat tacttggaaa ttataagttt gataagtata agacaaaaag taaggatttt
     2101 gaaagaaaag aagttgatat tataactata tttactgaaa aagaagttga gtgtcaaata
     2161 gaaaaggcta aaatattagc taattcaact ataatagcta gaaatctagt taatgaacca
     2221 tcaaacgtaa tatatccaaa aacattagca gtagaaacag ttaaacttgg tgcagagttt
     2281 gggtttaatg ttgatgttta tgaagaagaa aaaataaagg cattgggaat ggatgctttt
     2341 ttcgccgttt caagaggttc aattaataaa ccaagattca tagtaatgag atactttgga
     2401 gataaagaaa gtacagatat attaggtcta gtaggtaaag gattgactta tgatactggt
     2461 ggatattctt taaaatcaaa tgcttctatg ctagatatga agacagatat ggctggtgct
     2521 gcgagtgtta taggtgctat gtgtgctata tctcaaagta aattaaagaa aaatgttata
     2581 gcagtagtgg ctgcatgtga gaatgcttta tcaggaggtt catacaaacc tggagatata
     2641 attagttcta tggcaaagaa aactattgaa gttttaaata cagatgcaga aggtagactt
     2701 acattagctg atgcaatata ctacattata aaaaatgaaa aagtaacaaa ggtggttgat
     2761 gtagctacat taactggtgc tgctttaaca ttgttaggca atgttgcaac tcctatagta
     2821 acaaataatg atgattttta ttgtgaatta gaaaaagctg ccactttatc aggggaaagg
     2881 gtatggaaga tgccaattta tgatgaattt aaagctatga taaaaggaga agaagcagac
     2941 cttaaaaaca ctggtggtaa aaatgctgga tgtataacag caggagcttt tattggtgaa
     3001 tttgttggga atactccttg gatacatatg gatatagcgg ggacaagtac atcatcaaaa
     3061 tcaattggat acaaagcaaa gggggctaca ggagaacctg ttagaacatt gtactatttg
     3121 gcagaaatct aaaaaacaat tataaaatat aatttataaa gccacgtgga tatttttgta
     3181 atttaagatt atctatgtgg ctttattttt tatttaaata aattatttac tctttttctt
     3241 tttttcaagt ataaaagtat ctctattaaa taaaagagca ggtatgtaca taatactagt
     3301 aacaaaaata aacgtaaaaa tagcagttgt gctatgactt aaaaaaccat aatagtacct
     3361 agagttggta aatgtgttta gtaaaaatat gaatgaaaaa taaataaaac atatagctaa
     3421 gttatctcta agtgcatatc tgttaactct aaattttcta acatcaacca tatatatatc
     3481 tgcataaatt atcaagcact tagagaagat aaagataaaa tatattaagc taggaaattc
     3541 atagcccaag tttttagaaa aaatgagttc tccaactgaa caaactaaac tccaagaaaa
     3601 aaatatatta aaaagatgat actgcttaaa aattagtatg gctatacaca gatatataca
     3661 aatataattg attccaattg gtaaagagtt tagagtacta taattacctg aagaaataag
     3721 taataattgc tctagagcca taattattac aagtaatgta caaattatct tctcaactgt
     3781 atagctataa ggtaaaatat ttttggttag ttttggacaa tagtataaaa ataatccaaa
     3841 tataaagaga cttacaaatt gttcactaga aa
//
LOCUS       DAELJO010000289         3856 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.291, whole genome shotgun sequence.
ACCESSION   DAELJO010000289 DAELJO010000000
VERSION     DAELJO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.291"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            10..882
                     /locus_tag="KPB88_003698"
     CDS             10..882
                     /locus_tag="KPB88_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055932.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            879..2168
                     /locus_tag="KPB88_003699"
     CDS             879..2168
                     /locus_tag="KPB88_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055933.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVISNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKIISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2098..2742
                     /locus_tag="KPB88_003700"
     CDS             2098..2742
                     /locus_tag="KPB88_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0055934.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3009..3473)
                     /locus_tag="KPB88_003701"
     CDS             complement(3009..3473)
                     /locus_tag="KPB88_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0055935.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLRDGEFFVMRYQ"
BASE COUNT         1445 a          457 c          585 g         1369 t
ORIGIN      
        1 tatttatata tggaaatgaa aatagcagta tccaatctta gtaaaagatt tgggaaaaaa
       61 caagctcttg ataatataaa tcttgaaatc gacagtggta tgtttggtct tttaggaaga
      121 aatggagcag gaaaaaccac atttatgaga attttagcca ctttattatc aaaaacagaa
      181 ggttcaatac atatgtgtgg agttcctatt gagaatacta aggaaattag aaatatggtt
      241 ggttatttgc cacaagattt ttcaatgtat cctaacatga cagtatataa agcaatggat
      301 tatttagcag ttttatcgga tataccacta agaaaaagaa gaaaaattat agcaaagtta
      361 ttaagcaaag taaaccttac taattatcaa aatgtgaaag taaaatcttt atctggaggt
      421 atgaatagaa ggcttgggat agcacaagca ttaattcata atcccagagt tttaatagta
      481 gatgagccga cagcaggtct tgacccagaa gaacgtataa gatttagaaa tttactatgc
      541 gaaattgcaa atgatagaat cgttatttta tctacacata ttgttggaga tatagaagct
      601 acatgtgaaa acgtagctat attgaatagt ggaaaagtca tatacaatgg ctcaacagaa
      661 gcattagcaa aactagctga agggaaagta tatagtattg aagtagataa aaaagacatt
      721 gaaaatataa aaagtagatt tatagttata ggaatgttga ctcatggtgg aaaggctata
      781 cttaggatta tatcagatga taaacctttt gagacagcag taaattgtaa tccaactatt
      841 gaagatggct atatgcttat aatgggagga gataatatat gaatctattt ataaaagaat
      901 gtaaaaatat attaaagagt atggtatatt atatttttat tgtagtttta gtttttttct
      961 atatttctca acttggaagt tcagtttcaa atgatgtaag taaaaatatt cctaatgatg
     1021 gaaatccatt agttgctcca caaaaagtag agaatattga ggaatattac tctgtacaca
     1081 atcaatatcc atatggtcaa aaagaatcta tgaaaacaga aaaagtcatt tcaaatgcaa
     1141 ctataaacct aattagagaa tatcaaaaaa acacatatac aacgtatcca acaggtgtag
     1201 ttaagtatat aaaattagac caaaataaaa cgaagaccat agaaaaatat atagaagaaa
     1261 taactggtaa aattattagc gaaataaatt cactttggaa aactgatgaa agtataaaaa
     1321 tagattattc taaagtatct cttgaaagat ttgaagaaat aatgcaaaga gtagacgaca
     1381 ttttaggagg agggtctgca tatgcttttg acaagttaca aagatttgga gtagttccag
     1441 ttacctatga tgaagctatg aaaaattaca actatacaat acaaaaagat aaagttagta
     1501 atgggtatgc aagaattttt tgcgattata taggtatagt tttaagtata tttccagtat
     1561 ttgtggcagt ttttatgtca cttaaagata agcgttcaag aatgagagag attatttatt
     1621 caaaacaagt atcttcatgc aaaatagttt tatcaagata ttttgcactt gtctttatga
     1681 tgatattgcc tgtattaatt ttaggtatga aagatttact tcctttgatt ttctttgcta
     1741 aagataaagg tatatctatt gataacttag catttataaa atatacatta tggtggatac
     1801 taccaactct tatgatagtt acagctgtag gaatgagtat tactatatta acagatacac
     1861 cagtagcaat tattgtgcaa ttaatttggt ggctttacag tataaattct acaggactca
     1921 aaggtgatta ttcaacaatg ggactaatga taagacataa tagtgtatta aatggggata
     1981 ttattataca aaacttcaat caaattatga ttaatagaat tgtgataagc ttgggagcat
     2041 tggtgcttgt tttgttagct atattcttat atgagcaaaa aagaaggggg aatcttgatg
     2101 tttgggcaaa gttttcaaaa ctgtttagtt ctaataaaag aaaacttcaa attaagcact
     2161 ttaactaatt taatatgttc aattatattg ctatgtatga ttcctctaat acaaggagta
     2221 gctaatttag acagaatttc aacagctata tgtttagaaa attatgttgc aataataggg
     2281 atagtaatgt tggtacccgt attttcgcta gagcaatcaa aagaaattga tgagatagta
     2341 tcttcaaaag cattatctca agttattgta caattaatta ggatactgat gtcaattata
     2401 ggtatagtgg tattaatcat aggttttgta aatgttttaa tatatcttgg atgtaatttt
     2461 ccagtagttg agtatattat tggaagtttc gcatcagcta tgtttatagg aagtttagga
     2521 atgtttttct caaatttatt taataataca atcataggtt atatgatatc tataggatat
     2581 ttaattttaa atatgatgac taaaggcaag tatgttggaa aattttttat aatgtctatg
     2641 agtcaaggta gttttgatga gaaatattgg ctgatacttg gaagtgttgt gttgataatt
     2701 gcatctctct taataaaacc agtaaaaagg aagattagat aataaaatga gagcttagat
     2761 aaaataaagg taatacaaat aaaaagtaca tttatgaaat ataaatctat ttttatatac
     2821 acaaacctac ttacaatctc tttatatatg tagatagatt tatatatccg tagtataaaa
     2881 tatatatgta tacaaaattt tatcaaaagc tctcaaattt atatttcaaa gacacttatt
     2941 gctatggcct acacatatgt atttttttat tttaattgtt gtattaacaa taagactatt
     3001 gttataaact attgatacct cataacaaaa aattctccat ctcttaaatg cgttttttgt
     3061 accttagtct caactatttt aaatccttga gactcataac attttacagc tctcatgttc
     3121 caggttctta catttaatac aattggttta gtactatatt tatttctaca ttcttgtaat
     3181 cctaggctaa ttatttcttt accaattccc attccacaat aattcggttt cataccaata
     3241 ccaaagaata cagagcttcc ttcatctaat aagtttataa aacctatcaa ttctttattg
     3301 tcatttataa aacttataaa attttttctt ttatcttctt ttgctagtga aaaattcttc
     3361 tttttaattt cctcccatgg aggtagattg tatattgaat atttaccttc atatttccaa
     3421 ttagtaactt caatagcatg ttcttcagta aatttaactt tatttaaatt catatgtcct
     3481 cctattaaaa tatttaaggt aattaatggt attttaaagt tttagaaaat aagtaaaact
     3541 atttatattt atccataaaa tctttaaaaa ttttactaac aattcttgta ccctgtactt
     3601 tttattggat tcacagaaga ctataaacta attttgaatc accttctaat tcaaaaacat
     3661 cacgatatat ataatttctt agcttgatta aattctgtta aatccgtttc ttatagttgt
     3721 ataatcaaca tcaatatctt tatgtgtatt agttaattta ttggttttag gaacttatta
     3781 ttaatatatt gtatttgctc caataatcca agtagtaggt aaattatagg caaattatca
     3841 aatgtatatc tatcca
//
LOCUS       DAELJO010000290         3825 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.292, whole genome shotgun sequence.
ACCESSION   DAELJO010000290 DAELJO010000000
VERSION     DAELJO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.292"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(148..543)
                     /locus_tag="KPB88_003702"
     CDS             complement(148..543)
                     /locus_tag="KPB88_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0055936.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            684..1076
                     /locus_tag="KPB88_003703"
     CDS             684..1076
                     /locus_tag="KPB88_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0055937.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1176..1592)
                     /locus_tag="KPB88_003704"
     CDS             complement(1176..1592)
                     /locus_tag="KPB88_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0055938.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1607..2518)
                     /locus_tag="KPB88_003705"
     CDS             complement(1607..2518)
                     /locus_tag="KPB88_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0055939.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2700..3722)
                     /gene="splB"
                     /locus_tag="KPB88_003706"
     CDS             complement(2700..3722)
                     /gene="splB"
                     /locus_tag="KPB88_003706"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG0055940.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1275 a          656 c          429 g         1465 t
ORIGIN      
        1 tatcattatt gaaatacaca tctaataact aaaaattttt acatattcat tatttaaaaa
       61 tctttttatt gcatttaaat taaatctatc aatatttcag taataacgtt ttcttaattt
      121 tcactatgga tgtcgattca attataatta aagctctaca tcaatctttt taccactcat
      181 tgaacaacaa ctaacttttg ccttatcaat agagaatatt tcaaacttac catctcctac
      241 agagtacatc tgacctaaac ttggcattat ttcaagtgcc ttagcttgtg tttcttttga
      301 tgtaacaaat actgcttcac ctaaaatacg tagagtatta aggttttggt ctatacaaca
      361 aagttcaacc tttggattaa aaataagttg cttaaacata tccttttgat tagatgtgca
      421 aaatgtaagt ttaccattgt attccataac aaatcctaaa ggacgcacat gtgcttgatt
      481 gttctcatca actgttgcta aataataaac tcctgccttg ttcaaaaatt ctaatgcttt
      541 cataattctt acaccccttt taaatacttg tattaaggat tatcctttga tataatttta
      601 atataaaata cagaaaaaac aagtatgcag ttttttctga taaagtataa aaaagatact
      661 actataacta aggagtaatt aaaatgagta taaatactga aaaaaatata actaaaaacg
      721 ttgaagaatt aacctgtcca atccgttatg cattagacat tgtcggtggt aagtggaaat
      781 tacctattat atgtatgtta gcagttgaaa atccaattag atatagtagc ataaaaagaa
      841 aattggatgg cattacaaat actatgctag ctcaatcatt aaaagattta gagtctactg
      901 gtattgttca tcgtaaacaa tataatgaaa ttcctccaaa agttgaatat acattaacaa
      961 gtaaaggtaa aagtattgtg cctattttac aacaatttgc taattggggt gctactaata
     1021 tgcaagaaaa aaacacatgt ggacttagtt gcaaagaatg tagaaagata aagtaaattt
     1081 aaatttacta aattattcat attatagact atatattaat ttatctgatt gaatataaaa
     1141 acaggatgac ttatatattc atcctgttct atatgttaag ctctaaattt aattagttca
     1201 agcatatcgt ttaataccaa taaactatct acttgaatca ttagccattt acttccatta
     1261 aaaaagttta cctcattata tatttttttg atataatcac aacaagtatt aataaataaa
     1321 tcaacctcta ctctattgct ttcacttaca ataaccaaag ctttaaaata accttcttgt
     1381 ggatataaag tacatagtga tttacctctt tttttatatt ttacattcca acctctttgc
     1441 attgaacatt tactgtattc aagcttaggt tctacattat aagtagaaac caaagtttta
     1501 tttaacttgt cccataatgg attattaata aattcattaa tttgctcaaa tgtaggttta
     1561 gtatcttgaa aaaacgcatc ttgccaattc atattcatca tcctttctat tgttcaatag
     1621 gaaccggaac aatccaatgt tccagatatg ctgcttcagg tgtagtgggg taatacatct
     1681 caattgcaaa atcagtagtt gttaaattat gtttcttcat ccatctttcc ataaatttat
     1741 ctgctttaaa aacagctgaa ttaactaact cattaaagtt ttctgcctca aatccacata
     1801 ctaaatattc tttagctggc aactcaaaag tactatatcc ttctactgat gtacctgact
     1861 ctacctctac acctgccata tacatacaat ttccttcttt tgcatttccc atataaagag
     1921 cagcaaattc atttccacct ggaatttgat ttggtatatt atgtctttga cttccaagtt
     1981 tcatccataa ttcctctact attgcaattc cagttgtttc accgcctacc aactggcaaa
     2041 taggtacttc tatttctatt ccaacaaatg tccttggatt actcaatgtc ttacgattga
     2101 cttcaatcac tatatcatct gcaatcagtg gaacaccttc atcaaccata gcataattta
     2161 aaattaattc tggcttaata aaatgattga gttttactgg atttgctcta tatttttctg
     2221 gagttaatcc atatgctttt ttaaaggaac gtgtaaatgt ctcatgacta gcaaatccaa
     2281 aatttaaagc tacatcaagt atacgattct tatgttctgc taagtattca gaagctcttg
     2341 caagacgacg aagctttata tactccatta cagtcttatt taccaatctt ttaaatagtc
     2401 tttgatagta aaatggtgat aaatttgctt tatttgctaa ttcattaatt gatatttctt
     2461 ctgataaatt tgcttctatt aaatccaatg taatttgaat agactcccat gcatgcatac
     2521 tataactcct cctgatattt ttgttactct tatcctttat agtatcaaaa ctagaaaaaa
     2581 tgttcttgat agatagtgcc ctttttaatt ttcttatatg atagtgctaa atctcattgc
     2641 tttattataa acataaaata tataaaaata aaatatagca aatctttagg taagatttgc
     2701 tatattatat attttattaa actattttta aatactccat ttatttcctt aagaaagaac
     2761 tctttcatat catctaattg ttctttatca tacacgtact taccatatcc aaattgaccg
     2821 tatttaaact ttctatcttc attttccatt ggcaatagtg tatctggata aatctctagt
     2881 attctatttt ttgctttagt tgtatatcta tgagatataa cttcaaatat aattttttta
     2941 tcaaaatcct ttggtaaagc ttcttttata cttataatca gatttttata ctcttctttc
     3001 caaccttcat ataaaaacac aggtgctatt atgaaaccaa tctgataacc agccctagca
     3061 agttttacag ctgactctat tcttttattt gcagaggctg tataatgctc atgttcattt
     3121 ataattctag gagtattgat actaaatcgt atttctgtat gattattatg ttcagcatcc
     3181 aataagctat ccacatcact gtatttagta acaaatctaa atcttccttt ttcctctctt
     3241 ccaaaaaact ctattgtttt cttcaaagaa ttagtatatg gctctactgg aactgggtct
     3301 gatgtagctg ccccttcaaa tatggttacc tgtggctttc tatcttcaat atattttttt
     3361 gtctgttcaa gtatttcatc tacatttaca aacaccctta taaatggttt atcacctaat
     3421 tgtgtgttca aataacaata ctcacatctt cccatacatc cactgacaag tggtagttga
     3481 taattagcag aaggtttaca tgtttgaaac ttcagacttt ttctaatccc aactaccata
     3541 gtctgcttcc cacttctata ttgttcatat agattttcac ctggaatatg ttctttaatc
     3601 ctatttgatg atatattaat tatctctaca tctttattat ctctaaattt attgtatatt
     3661 tttttaccca catcagtgtc taaagttcct ttttcgaata ttattctttt gggattaaac
     3721 atttatttca cctctactta aagtttttaa atcgaagaat tttataaaat attacttaat
     3781 taatcttccc ttttttaaac tcctttattt attagaaagt aaaat
//
LOCUS       DAELJO010000291         3821 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.293, whole genome shotgun sequence.
ACCESSION   DAELJO010000291 DAELJO010000000
VERSION     DAELJO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.293"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            95..1147
                     /gene="ytvI"
                     /locus_tag="KPB88_003707"
     CDS             95..1147
                     /gene="ytvI"
                     /locus_tag="KPB88_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0055941.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1237..2463
                     /gene="pepT"
                     /locus_tag="KPB88_003708"
     CDS             1237..2463
                     /gene="pepT"
                     /locus_tag="KPB88_003708"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG0055942.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2670..3722
                     /locus_tag="KPB88_003709"
     CDS             2670..3722
                     /locus_tag="KPB88_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG0055943.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1480 a          401 c          700 g         1240 t
ORIGIN      
        1 atatttatac acttcttttt attttgtatt caaataaaac tagtatgaaa tggacaaaat
       61 aaaaatataa attagaagaa aggagatttt taagatgagc atactagaaa ataatttttt
      121 atacaaatta aaaaacaaca ctatcttttt ttgtatatat acattgttat ttatttttat
      181 ttacaaagcg tttccataca tagctccatt ttttctaggt gctataatag cacttatgat
      241 taatcctata tcacaaaagt tagaaaataa gtttcgtata aataaaggta tctctactct
      301 tgttctaagt tttcttgcag tggctatagt cagtacagtt actacaataa tagtaataaa
      361 ttcaatgaaa gaattaatgg gatttttaaa taatatatct gctaatcctg aggatataag
      421 taatactatt atgtattttt taaataaaat aaatgacttt atgaaatcat ttcaagaaat
      481 agccaatttt gatgtagaac aattagtaaa caaattcagt ggtgaagtaa tgcaaattac
      541 aaaaaatctt cttactagta tacttggatt ggctacttct attccatata ttataatatt
      601 tataattacc ctgtttattg ctacctactt tatagcaaaa gaccttgata agattgaaaa
      661 tagtttttat aatatgttta ctgttgatgt tagaaagaag gtaaaaaacg taaagaaaga
      721 ggcagggctt tcacttgttg gatatataag ggcctataca atacttatgg ctataacatt
      781 ttttgcaatc tggggaagtt ttgctttatt tggattgaaa tacggtctaa tagtagggtt
      841 tgtaggagct ttaatggatt taattccatt cctaggtata ataacaatat atcttccagt
      901 aattgtttat tattttctta taaaaaatta ttttatagct ataagtatga ctgtgatatt
      961 ttttgttttg tctttgatta gagaaatact tgaacctaaa ctagtatcag taaatgttgg
     1021 actaaatcca ttagctaccc tagcagcgat ttttattgga atccaagtaa aaggaataat
     1081 tggagtaatt ttttgtttgg gtttggtatg tatgcatgat atacttaaga aggtggatat
     1141 tttttaatta taataattga tagatttttt aaaaataaaa ttttattcac aggtaaataa
     1201 aaaaatatta atcttattaa gaaagagggt atatacgtga aacaaaaagt agtggaaaga
     1261 tttttaaaat atgtatcatt tgatacaaca tctaattctc agtgtgagaa ctgtccatct
     1321 tcagaaggtc aaagagtttt agctaagtat atagttgaag agctaaaaac aatgggtgtt
     1381 gatgatgtga gtttagatga aaatagttat ataatggcta ctttaaaagg aaatactgat
     1441 ggtgtggata ctataggatt catatcacat ttagatacaa ttgaagatgt tagtggaaaa
     1501 gatattaaac caagaataat agaaaattat gacggaaaag acatagtttt aaatgaagcg
     1561 ttaaatgtta ttacatatgt aaaggattct ccagagttgg aagaatttaa aggtgacgat
     1621 ttaatagtca ctgatggaac aactctactt ggctctgatg ataaagctgg tatagctgag
     1681 atagtgactg caatagagta tctaataaat catccagaaa ttaagcatgg agatataaaa
     1741 ataggattta caccagatga agaaattggt agaggtgctg atttatttga tgttgaaaaa
     1801 tttggagcaa aatatgcata tactttagat ggaggaatag ttggagaact tgaatgtgaa
     1861 aattttaatg ctgcaaatgc aaccattact atacatggta gaaatgttca tccaggttca
     1921 gctaaaaata aaatggtaaa tgctatacat atagctgctg aaatatcaga aatgtttcca
     1981 gcagatgaaa gaccagaaac aacagaagga tatgaaggat tttggcattt aaattctata
     2041 ggtggaaatg tcgagaatgt aagtatggct tatattatta gagaccattg tagagaaaaa
     2101 tttgaaaata gaaaatctat tatgatagag aatatagaaa aaataaataa gaaatacgat
     2161 aatagagtgg aattggattt aaaagactct tattacaata tgaaagaaaa gattgagcca
     2221 gttatgttta tagttgatat agctaaagaa gctatggagg aattaggtat aaaaccacgt
     2281 ttagttcctg taagaggtgg tactgatgga gcaagattat cttttaatgg attaccttgt
     2341 ccaaatatat ttactggtgg attaaacttc catgggaaaa atgagtgtat accagttagt
     2401 tctatggaga aagctactaa actaattgta agaatagctg aaaaatatgc tgaaagagtt
     2461 tagttaaaaa gtatttaaaa attaagcatt taaagttata atagctatat cattctttat
     2521 aagtcaagct gattataagc ttgtgttgta cttaaaatgg tatagctatt tttgtaatta
     2581 tttttataaa agataattat ttttagttta gggtatcaat aaaaagtatg aatatcaata
     2641 ggtattaaca tttaaaaggg ggaattgtat tgaaaattaa atctaaaaaa ataatagcat
     2701 ttgcactttc acttgggatt atgttgccaa gttgtgtata tgcagacact attagtaaaa
     2761 atcaaaatgc acaggtagat atgaacaatt atcttcaagg tagaaaatct ccaaaggagg
     2821 aaaaacttat tggtgaagat agatttgata cagcgataaa aatatctcaa agtggttgga
     2881 ataatggttc agaaagggtg tttctagtta actcaaattc attgccagat gcattggctt
     2941 caactccttt ggcaagtaaa ttggatgcac caatactgct tacaaataaa aatagtatac
     3001 catataatgt aatagaggaa ataaaaaggc ttaatccagc agaagttatt ttaattggaa
     3061 gtgaaggagc tatttcatca agtgtaaaag aatctcttga agatatggaa gtattagtca
     3121 gtagaatcgg tggagcagat agagaagaaa catctttact tcttactaga cagctagatg
     3181 atactggtga tttaggtgtc tcaaaagtgg cagtggttaa tgggtataat ggactggcag
     3241 atgcaacttc aatatcaagc ccagctgcaa gtgataatac agctataata tatacaggta
     3301 aggatagcat aagaagtgag gcaaagaatt ttattacaca aaactcaact aagacataca
     3361 tagtaggtgg ggagtacagt ataagtaaaa aacttgaagg acagttagta aactcagaaa
     3421 gactagcagg aacagataga aaagatacta atgcgaaagt tttagaaaag ttttatggaa
     3481 aatcaagtaa ggttaataat atgtattttg caaaagatgg aagtggtaga gaagctgatt
     3541 tagttgatgg gcttgctgct ggagtattag catcaaaaac aaagtcacct gtagtacttg
     3601 caagtggaag tttaagtagt gcacagaaaa gttttataaa aaaagtaaag gctgataaat
     3661 ttgtacaagt tggtggaggt aaaaatagta aaccatatgc agaagcatta gccttacagt
     3721 aatattgtgt aaatcaaaat taatatataa taatagcaag taaaccaagt tgcacttcaa
     3781 tatatataat aaaataagta aactaagtta tatttcagta t
//
LOCUS       DAELJO010000292         3810 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.294, whole genome shotgun sequence.
ACCESSION   DAELJO010000292 DAELJO010000000
VERSION     DAELJO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.294"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            57..1010
                     /locus_tag="KPB88_003710"
     CDS             57..1010
                     /locus_tag="KPB88_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0055944.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFMFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRESIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1000..1956
                     /locus_tag="KPB88_003711"
     CDS             1000..1956
                     /locus_tag="KPB88_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0055945.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1953..2708
                     /locus_tag="KPB88_003712"
     CDS             1953..2708
                     /locus_tag="KPB88_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0055946.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2800..3765
                     /locus_tag="KPB88_003713"
     CDS             2800..3765
                     /locus_tag="KPB88_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0055947.1"
                     /translation="MNKKAAIVATVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1534 a          362 c          651 g         1263 t
ORIGIN      
        1 agtgaaaatg ataataattt tcattttaat taataaaaga tttttaagga gggagaatga
       61 aaaaaaggta tttagtcata ggaataataa ttttgtctta tttttcttta tttataggag
      121 ctgaagatat aaatataatg catatatttg caaaaaatca gcataagctt atgatattta
      181 tcatgagtag ggtaccaaga ttaataagta tattgattgc tggtgtagga atgagcatag
      241 caggtcttat aatgcaacag ataagtaaga ataaatttgt atcacctaca acaggagcta
      301 caatagatgc agctcaattt gggatagtaa tatgtatgct tttagtacca actgcctcta
      361 tttttacaaa gacaataata gcatttgtat tttctcttgt agggactttt atgtttatga
      421 aaataatagg gaagttacaa tttaaaaata taatctttgt accattagta ggtataatgt
      481 ttggaaatat aataggttct atgacggatt ttatcgcgta taaatacgat ttgagtcaaa
      541 atgttagttc ttggatgcaa ggtgactttt ctatgatact taaaggaaat tatgagatat
      601 tatatatcac aatacccctt ataatattag catatattta tgcaaataaa tttactgttg
      661 ttggtatggg tatggatttt gcgacaaact taggtcttag ttacaaaaga attgtcaata
      721 taggtttgat aatagtagca cttgttacag tatgtgtagt cgttacagct ggtaatatcc
      781 cttttatagg tctaatagtt cccaatatcg tatccttgta tatgggtgat aatataagag
      841 aaagtatatg gtatactggt ttacttggag caatatttgt acttatatgt gatatttttg
      901 gaagaattat tatttatcca tatgaaatat ctatagggct tacagttgga gttataggaa
      961 gtatactatt cctttattta atattaagga ggaacgtaaa tgaagcttag tctcaatcaa
     1021 aaaatttact ttgtaagtat attactattc atatcaatag cactttttgt aatttatgga
     1081 atagacatga atcacttaga atatgcatta tcacaaagaa taccaaaaat atttgctatg
     1141 gtattaggtg gaggatgtat agcatttact actgtagtgt ttcaaacaat tacaaacaat
     1201 caaatattaa caccaagtgt actaggttta gattcattgt atgtaatgat tcaaactata
     1261 atagtattca tatttggttc aagcagtagt ctgattataa atgaaaatta taattttatt
     1321 attaatgtag ctatgatgat tggagcttca ttgttattat ataagacatt atttgaaaaa
     1381 aataaaaata atattttctt tttaatccta gtaggaatga tatttggaac cttatttaaa
     1441 agtgcaacaa catttattca ggtaatgata gacccgaatg aatttttagc tttacaaacc
     1501 agtataatgg caagtttaaa caatataaac actaatgtat tgttaatagc atttataata
     1561 attattgcca taataccttt tatatatgat gaaataaagt atttagatgt attgtcgtta
     1621 ggtaaagaac aagcaattaa tttaggtgta gattttgata aagcagttaa aaaaatgata
     1681 atattaatag caattttagt ctcaatatca acagcactta ttggacctat gacattttta
     1741 ggacttttac ttgctaatat aacaagagaa atttttaaaa catataagca tacatacttg
     1801 atttcgggtt ctatgttgat agggatgatt acattgataa taggtcaatt ttttatacaa
     1861 catgtattta agtttgatac aactttaagt gtagtaataa attttatagg aggaatttac
     1921 tttattcaat tactattaaa gggggcaaat agatgataga gataaaaaat atatttaaaa
     1981 gatataagaa taaaaatgtt gtagatgatg tctcttttag tatagaaaag ggaaagataa
     2041 catcttttat aggaccaaat ggagctggaa aaagtacagt actatcaata gttacaagac
     2101 ttataggagg tgatggtgga gaagtaataa tagaaggaaa aagcttgact aattatagca
     2161 ataaagaatt agcaaaaaaa atagctattc ttaaacaatc taacaatata actttaaagc
     2221 ttacaataag agaattagtt ggatttggaa gatttccata tagtgaaggt aatctaacta
     2281 aagaggatga aaattatatt gatgaagcaa tagaatatat gaagttgact gatatacaac
     2341 ataagtattt agatgaattg agtggaggtc aaagacaaag agcttatata gcaatggtaa
     2401 tagctcaaga tacagagtac atattacttg atgaaccact gaacaatttg gatatgaacc
     2461 attccgtaca gatgatgaag gtacttagag gtctttgtga tgagttagat aagactatag
     2521 tattagtaat gcatgatata aattttgcat catgttattc agataatata gttgcattaa
     2581 aaaatggaaa agttaaaaaa gtaggtcgta cagatgagat agtaaatgaa aaagttttag
     2641 aagatattta tgagatgaat tttaacatta agaatataaa tggaaatcga atctgtattt
     2701 atttttaaaa tattgtatat ggaaataaac ttgctatatg attttttata aagtttttaa
     2761 attaaaataa agattaatag gagagaggaa aatattgata tgaataaaaa agcagcaata
     2821 gtagcaacag tagctattat aggattagtg acagtgtttg cattaggtgg aagtaaaaag
     2881 aatgaatcaa aaacatcaga aaattcaaat aatactatga agataactca taacttggga
     2941 gaaacagatg taaaattaaa tcctaaaaaa gtagtagtat ttgattattc ggctttagat
     3001 acaatggatg cattaggtgt agctgagaat ttagtaggac ttccaaaagc gagtttacca
     3061 gcatccttag aaaaatataa ggatgacaag tatgcagatt taggaggctt aaaagaacca
     3121 gatttagaag gtataaagtc ggcaaatcca gatttaatta taataaatgg aagacaagaa
     3181 gatttttatg agcaattgtc aaaaatagca ccaacaataa gtacaagtaa agatgataag
     3241 aagtacttag aatctgttaa aaataatata gacaaaatag caaaaatatt tggagtagaa
     3301 gaaaaagcaa atcaagaatt tagtaaaatt gaaaagaaaa tagaaacttt gaataaaaaa
     3361 gtaacagata aaaatttaaa tgctttaact ataatggtaa atgaaggtaa tttaagtgta
     3421 tttggtgaag aatcaagatt tagtatatta tacaatagtt ttggatttga aaataaagat
     3481 aaaaatataa aagaatcaag tcatggacaa aatattactt ttgaatatat agctaaacaa
     3541 aatccagaag ttatgttcgt tatagataga ggaatagcta ctggcagtga tgtaaaagaa
     3601 agctcaactg caaaatctgt tttgaacaat gatataataa aatctatgga tgcatataag
     3661 aatgataata taatatattt agattcacca acatggtatg taaatgatgg tggtctaaca
     3721 tctttaaata aaatgataga tgatgcttca aaagcggtaa attaataatc cgatataatc
     3781 aaaatgtatt ttttataaaa tgattatcaa
//
LOCUS       DAELJO010000293         3790 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.295, whole genome shotgun sequence.
ACCESSION   DAELJO010000293 DAELJO010000000
VERSION     DAELJO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.295"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..238
                     /locus_tag="KPB88_003714"
     CDS             <1..238
                     /locus_tag="KPB88_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055948.1"
                     /translation="IKHKVNYCNKKSLDKLELKYGNIDREKAVKLEIFYQYLIELEYI
                     EMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   339..1028
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=406..434
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1687..3780)
                     /locus_tag="KPB88_003715"
     CDS             complement(1687..3780)
                     /locus_tag="KPB88_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0055949.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1358 a          582 c          369 g         1481 t
ORIGIN      
        1 aattaaacat aaagttaatt attgtaataa aaaatcttta gataaattag agttaaagta
       61 tggaaatata gatagagaaa aagctgttaa actagaaata ttttatcagt atttaataga
      121 acttgaatat atagaaatga gtttattaat aagaagatta gatactgcaa taacatcatt
      181 agcattagta tctattataa caatagcatc atattattta atcagaaaaa atgtgtaatt
      241 atgtaagcta tttataataa aacactgata aaattaataa taaaaaaggt gataagattt
      301 ctatattacc ttttgacttt tataatatag tgtaatttta ttatattttt gtatgctaat
      361 ataactatat aatcataaaa taacacctta aaaaaaatta taaaaattta cattccatat
      421 agttaatata aaacacttgt aaaattaaaa gatgaagtta gtccaacttt gagatttaca
      481 ttccatatag ttaatataaa acattcttgg gttgaaaaag attttagcaa ggacgtaact
      541 ggatttacat tccatatagt taatataaaa ctcccgctat ttttgatatt acgcttaaca
      601 tttttcaatt tacattccat atagttaata taaaactttt gaaagtgata atgctgtttt
      661 accgctttcg aaatttacat tccatatagt taatataaaa cgaaactaaa acctgctatt
      721 gcaaaacata gacgaacatt tacattccat atagttaata taaaactttc tcctgcaagt
      781 aacattcttc ttaaaagttc tttatttaca ttccatatag ttaatataaa accttggcac
      841 tattatttgc acaggataag gctgatggat ttacattcca tatagttaat ataaaacgat
      901 aatatacgtg attatgatgt cgcctttaat tatatttaca ttccatatag ttaatataaa
      961 actaccatgt gatttacaat tttcaaaatc taaattcata tttacattcc atatagttaa
     1021 tataaaaccc caaaataaac ttagtatttt caacacttac acgtatacaa ctctcttaaa
     1081 tttgcagtga acgagcaata gtgcaattga taacgcttat catgcactct caacgcctta
     1141 tatttcaact gttacgccaa attttttcac aaatatcgct cactgcaaaa ttcctataat
     1201 tttattatat cataaatata ttacttttga atattgatac caatttatac cataataaaa
     1261 gcaaaaaaac gtaatctatc tacattagat ttaaattcta taacttaaag ttttttacat
     1321 aatatttatg cttgtttaat caatctaata tatcattctt gtatattgct tttaatagtc
     1381 ttttttctta aataaatatc tatctgaaca agcatagttt aattaaaaac ttttgtattc
     1441 ccttaaaact gtatctacct cttattattt tatttatgat atattatttt taatctaaaa
     1501 atagatatct atttttatta tggttatccc tttttaaaat actttaatca tatactattc
     1561 ccaatttaac atccattgaa atgctatttt cttatattac aatttataat tgatatatct
     1621 tcaatataat aatttaatgt atttttgatt ttaaagcttt tcagtgatat tattgataaa
     1681 ttagaattaa ttatctagct ttaaaaggtc aaagatttct gttgctgtct gcggttttcc
     1741 aaataaatat ccttgtatgt aatctggctt tatttgttta actaaatcat actcttcttg
     1801 tgtttctaca ccttctaaac atacttttat tcctacgtca tgacaaattg caacaataaa
     1861 atgtataaat gtagcatcaa acttactctt taatatatct ttaacaaata ctctatcaat
     1921 tttcactata tctattggag aaaattttaa aacttctaga gacgagtatc ccgtaccaaa
     1981 atcatccata gcaatataaa ttcctaaatc ttgtaaagcc ttaaatttaa actgtaaaat
     2041 actcatattt tgtactgtat gactttctgt caattctaaa acaagatttt gataaggaaa
     2101 attttcatca tctataattt ttactatatc ttcaataaat gtgtcttcta atatttgaac
     2161 agctgaaacg tttacactca ctttaaaaaa aggatagtaa tcaatccatt tcctaaaagt
     2221 acgtaatgcc attcgaagca cccaagcccc tacaatattt atcatatcat tttcttctaa
     2281 tattggaata aattccaaag gagaaattgc tttgcatttg tcattagtcc accttaaaag
     2341 aacttctaca cctattattt tatgactttc tgtatctact tgtggctgga atcgaagact
     2401 aaacccccta aaatcatcat taattgatgc ctttaaatcc cgcatcattt ctaatgaata
     2461 cattttattt tttaatattt cttgggagaa aaaaacaagc ctgtttttac catgctcttt
     2521 cgcatattgt aaagagtaac tagcacactt ataaagttct tttactgtat ctccatgctt
     2581 tggatatatt acacaaccag cagaaattgt tatatttaat ccatattttc tccatagttg
     2641 aaggtgtata atcatattct gtatttgatt atatagagtt agaatttcat tttcttcaac
     2701 attatcaaca agaataccca tttcgtcccc atcaagttta taaagttcag cattatctgg
     2761 taaaatagat tgtatagact gtgctagtgt ttttattata ttatctccaa aatccctatc
     2821 atacatctca ttaacatttt taaaatcgtc aatatttaat aatactatgc ataatttttc
     2881 aatcatagga tttgaaattt tatcttcaaa tacactcata aattcatgat aattcagtaa
     2941 ctgtgtcaat gaatcaatct tattttgttt acctaattgc gtcattattc ctgcaaaaat
     3001 acttggttct ccaaattcat cacgcattag ttgacctcta catcttaacc atatatattc
     3061 tccactacga tttttagccc taaattccac tgtatgataa tccatctggt ttttacctat
     3121 ttcagtgttt gacttataaa aacgattcca gtcttctgga tgtacaattt ttttccagta
     3181 aacaagcgga ttttcaacaa tttctcctgg taagtcaaat aattcaactt gtgatggtga
     3241 atatcgaaat acgcctgttt tcatattgca aatgtatata tattcatctg tacctctaat
     3301 caaagcatca taaagcaatg tagaattata atcaaaattg tctcttgaaa aattaaaaac
     3361 tcctttttct tctttattat ttgatatttc atgaaattca ttaatcttaa ttggtttatt
     3421 taagcttgtt gagtttgcaa tatctattga ttgatgcata tgaacaactg tgccatcttg
     3481 ccatgtaatt aaactatcat aattttcaaa aacacgattt aacttattac atttttcata
     3541 ccactttatt aatactcctt ttttatcatt ctttaataat tctaaaactt tacaaaaact
     3601 acaagtagaa tttttttcag gatataatac ttgccaacaa acttttcctt ctggatctaa
     3661 aatattatat tcttctttca tttttttgtt cataaaaata atttcattag tatgaatatt
     3721 tgtaacatat atattaatct gtagttgatt taatataact tcaaaattat gtttgttcat
     3781 atacttatct
//
LOCUS       DAELJO010000294         3789 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.296, whole genome shotgun sequence.
ACCESSION   DAELJO010000294 DAELJO010000000
VERSION     DAELJO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.296"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            313..1689
                     /locus_tag="KPB88_003716"
     CDS             313..1689
                     /locus_tag="KPB88_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG0055950.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1976..2647
                     /locus_tag="KPB88_003717"
     CDS             1976..2647
                     /locus_tag="KPB88_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG0055951.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2836..3633
                     /locus_tag="KPB88_003718"
     CDS             2836..3633
                     /locus_tag="KPB88_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG0055952.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1451 a          414 c          690 g         1234 t
ORIGIN      
        1 atttaacttc aaaaaagtat atgaaattta tactggtaaa atgataaata aattttaagt
       61 acaaaatagt aaaagctatg atagttttag acctattata tgtaaaaaat atagatgttt
      121 ttggtgcaat ttaaattaaa agatatatat aaaaatttaa aatattccaa acagaaatta
      181 taaaaattac tatcattgtc tttatacaat atttatacta taatagaata ataatttaga
      241 ggaaagctct aaaaataaac aaattaaggg tcttaacagt agtgatttta tgttaaggag
      301 gtataaagaa agttgaaaac aatagttaga agacttattc tctacgcaga tggaatgcgt
      361 cctactcaaa taatggtatc tgggtttgct gcgattattg taatcggagc attgttgtta
      421 acactcccga tagcgtcgca gagtggagag agtataggct tattaaatgc tctatttacg
      481 gctacttcag cggtttgtgt tactggtcta gttatggtag atacagcaac ttattggagt
      541 ttatttggac agatagttat aataacattg atacaaattg gtggtcttgg atttatgacc
      601 gtagccacaa tgttttcact tatggcacga aaaaaaatac aactaagaga aagattatta
      661 atacaagagt cattaaatca agctgattta tcgggacttg taagacttac aagatttgta
      721 ttaataataa ccataacaat agagggaata ggtgcactgg ttttatcaac tgtctttatt
      781 ccacaatttg gtttgtctaa agggatatgg tatagtgtat ttcacgcaat atcagcgttt
      841 tgtaatgccg gatttgactt gatgggcagt gtaagtggcc catttacatc gttaaattct
      901 tatgtaaaca actttacagt atcaatgact gtttgcgcac ttatagtgct tggagggcta
      961 ggattccctg ttgtacttga tattgtaaga aaaagaagat tttcaaaatt aaatgtgcat
     1021 tctaaggttg tattgttttc aactgctaca cttatttttg tgggtgcatt atttattttt
     1081 cttatagaat ttaatcaaaa agctactatg gcggacttgc ctttaaaggg aaaagtacta
     1141 tcagcaatat ttcaatcagt aacagcaaga actgctggtt ttaatacgct tgatttagct
     1201 actttacgag aaagtagtgt atttgtaatg ataattttga tgtttattgg agcatcacct
     1261 gcatcaactg gtgggggtat aaaaacaaca acactagctg ttttaatcat tacagttaga
     1321 agttttctat caggaaaatc agatatagaa gcttttgaaa gaagattagc tccatcaact
     1381 ataaaaaaat ctcttggtat atttgtaatc agtatatcag cagttatttt tggaacattg
     1441 attatttcaa taactcagcc taattttaca ttagttcaat cagcttttga ggttacatca
     1501 gcacttgcta cagttggttc aagtttagct ggaactccaa acttaaatgc tttaggtaag
     1561 attataataa taatctttat gtttatgggt agagttggtt ctcttacttt atttatggct
     1621 atactttctg gtggtagaag aaagagtcaa ccaatcagat atgcagaagg taaaattatg
     1681 gttggttaag ttaccaagga gtaatataaa tttataatgg atttagagta gcactgtaaa
     1741 tctgttgata aaataaatga gcctagatgt aattatatga agaaataaaa tcgactaacg
     1801 aacaaaatta attaatgaaa taaaaaaaca gggaaataaa atacaaataa aaaggtaaat
     1861 caaaattaaa ttaatctgtg tatataaaat tatatattgt gtataagttt tgtatgtgta
     1921 aacaattaat ttaagagata ttatataaat tgtgtattag ggaggaagtt tcgagatgaa
     1981 gcaatatata gtcattgggt gtgggagatt tggaagttca gttgcgtcta ctatgcatct
     2041 tttaggacat caagtaatgg caatagacaa aaatgaagat tcagttcaaa gtatatctga
     2101 caaggtaacc cattcactta tagtggatgt tactgatgag caagcgttaa ggtcattagg
     2161 tttaggtaac tttgatgtag cagtagttgc aataggttct gatataaggg catctataat
     2221 ggcgactctt atagccaaag aaatgggtgt agagttgata atatgtaagg caaaggatga
     2281 attacaagct aaagtgcttt ataaaattgg cgcagataga gttgtatttc cagaaagaga
     2341 tatgggagta agagttgcac acaatttagt ttcagataat atattagacc atattgaact
     2401 tgacccagag tattcaattg ttgaaatcgt aactccaaat agttgggttg gcaagacact
     2461 tatagagctt gaattaagag ctagatatga gataactgta cttgctataa aaacaggtaa
     2521 aaatataaat gttacacctt ctccagatga ggaacttaca gccggaagta tcctagttat
     2581 aatcggtcaa aatactagta taacagcgat aacatctgga aataagggga taattagaag
     2641 aagataattt actatttaat atatatttaa ttgcaatgaa agtagatagt atcatataat
     2701 tatgaatagt tatatgatac tattttttta atcgaaggta gtatttattt gtaagattag
     2761 ataaagagaa attaaattaa aagtaaggag gctgtgctaa tataaaaatt tataagttat
     2821 tagcatttag ataaaatgat tacaaatata aacagtaagg ataatgaaaa gttaaagtat
     2881 acaagagcac tattaaaatc aaagaatagg aataaagagt caaagttcat aatagaggga
     2941 tacagaatag taatgcttgc acttgaatgt atggcaaacc ttgattatgt atttatcaat
     3001 gaagaatttg aaaataagaa agaacatgta aaactattag aagatttgga taaaaaaaac
     3061 acaaagatat acaagactac taataaaaac tttaaagaat tagtggatac agaaaatact
     3121 caaggaataa taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag tataaataaa
     3181 aaagataaat ttgtattgat tttagacaga atacaagacc caggaaatat ggggactata
     3241 ataaggactg ctgattctgc tggagtagat gccataatag cactaaaggg atgtgtcgat
     3301 atatacaacc caaaagtaat taggtctact atgggttcta tttttgatgt gaatataatt
     3361 gatgcttcac aagatgaaac tgtggacatg cttaaatcat tggattttaa tatagtttca
     3421 agttacttaa atacagaaaa tttttatgac aaaatagatt atggctcaaa agtagcattg
     3481 gtgataggaa acgaagcaaa tggaataaat gaagaacttg tatcaaagtc tgatattttg
     3541 gttaagatac ctatatatgg taaagccgag tcgttaaatg ctgcgataag ctctgctata
     3601 ctgatgtatg aaataaaaaa atacttaatt taatgtattg taataaatat aatgtcatgt
     3661 tataatcttg aattaaatag tagagtatca aaaatagtca atatatatta aaaaaataat
     3721 taaattaatt atatattaga tgtatattat ataaaaaata attgagttag ttatgcatta
     3781 gatgtatat
//
LOCUS       DAELJO010000295         3638 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.297, whole genome shotgun sequence.
ACCESSION   DAELJO010000295 DAELJO010000000
VERSION     DAELJO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.297"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(146..682)
                     /locus_tag="KPB88_003719"
     CDS             complement(146..682)
                     /locus_tag="KPB88_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG0055953.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFDSDIEEEN"
     gene            complement(700..2013)
                     /locus_tag="KPB88_003720"
     CDS             complement(700..2013)
                     /locus_tag="KPB88_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG0055954.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2368..2808
                     /locus_tag="KPB88_003721"
     CDS             2368..2808
                     /locus_tag="KPB88_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0055955.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2993..3181)
                     /locus_tag="KPB88_003722"
     CDS             complement(2993..3181)
                     /locus_tag="KPB88_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0055956.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
BASE COUNT         1249 a          634 c          351 g         1404 t
ORIGIN      
        1 gaatataatt ataaaaataa aaaaagaata ggataatttt cctactcttt tttctgtact
       61 tacatataat attaaaactc tttatttttt aatatatatt ttaaattttc ttttttaata
      121 aatctttttc ttttaataaa tctctttaat tttcttcttc tatatcagaa tcaaagggaa
      181 tccatgcatt accaaactct ttatctttaa ataaataagc taatccagtt atttgcttca
      241 atctaagaat ttcatcaact tccataccca ggtgctttga tatccattca tcagattttc
      301 caatttcaca aagttcagtt ataattttac tcattaaatc cacattatga gaacctcttg
      361 cacgattgtg tcgaatagta cttgccattc tttcatcaag agatttatca attactgaaa
      421 caggaagaag tccgttttct ctatcatata tatcctcatt ttcaagcatt actctatatc
      481 tatgaaaacc atctacaata atatacttat ccttttcctc attataataa cagacaattg
      541 gcattgtata cccatcttct tttatacttt tatacaacaa ctgcatttct ggtggggcta
      601 ctgcatttgg gttatagtcg ttagattcta tcttttcgat tggcactgcg ataattccat
      661 atacaggact tttatattcc atagcatctt aactcctatc taatgaattt tttcatattt
      721 cttcttaata tattccgttc taattctctg ttcttttgta ggaccaaatc ccataaatct
      781 acacatatgg tcatttttta aaatacaata acacattctt ttccaacttg gtatatcttt
      841 ggtaaattta atatcatccg tatcatcagg tatatcatcc aaaaatatga ctctactctt
      901 tttatctttt gtatgatttg aaactccatt taattctatt ttataacctt ttctcaacaa
      961 ttcacgtata actatttctg acaaaccacc acctgtattc tgccaaaact caatagatgt
     1021 tgaaaatttt ttaatatagc tattccttat attttcaggt aaggtatcaa gcaaaaaata
     1081 tgtgaaactt ttccatgtgt gtccatatgg caatttaaca ctacgatatc ccatagcttt
     1141 tgttttgcca taaatagaag taaaattaac tccttgtact ctagcaacca atttcaccca
     1201 tacatctggg tccaatattc tgtataaatt taaactctct tttgctgaat cattaaaagg
     1261 tgatgccact ctcatttttc ttggagctac tccagcttta tagaataagt cataaatttt
     1321 attatagtca tagtcaaatt tatagtgtgc taaccatata tcttctattt cccaatcata
     1381 tataggagat gccgtccaga catttttaaa ttccttagtt atccagcact ttcctttata
     1441 tccatgctgt ttattcacaa tagaacaata acgttgtaga gattctgctg ctcgtgttcc
     1501 taataagcaa acaacttttg caccttcatg tatatctcta atccatctac caaattgttt
     1561 tgccaaactt tcttgtctca ttttatattt ataataagta aaaggattat ttttttgatt
     1621 tattatgtat tctttgactg gcatttctct tacccataac tcttctttct catcatccca
     1681 tggataccaa tacatttcat aatttcctac agcagttctt gtagccattg gtaaacacac
     1741 ccaatatggt tcaatatctt caatattatc ttcaaatact ttttcaacat attcagtagt
     1801 caaactatat tgagcctcaa aatcttgatg aaatactcct attttggaac ttatattatt
     1861 ctttctctta taatccaaaa ctaaatttag cagtaaacca ctatcttttc ctccgctaaa
     1921 agatacatag atataatcaa attcattaaa aagataagaa agtctttctt gaaaagcttc
     1981 aaatacatcg gattctaaat atctcttatc catttgaatt atcccctttc aattagaagt
     2041 ttataaaacc ttacaatgca atattattat gcttttttag cagtaatcag cataaattat
     2101 acattattta aatacacaaa tccaactaaa tacatataat tataatgtaa ttatactttt
     2161 gtattataaa ctatatttta tcattatttt acattttttt ccaatatata taattatcat
     2221 atttttctat tagctaaaac taattacctt atatttttac gtattggtat aataatacat
     2281 aaaaacttga ataatgcata aaaacttgac atactgttgt cataattact ttgctataat
     2341 taaaagtaaa aattgaggta aattctaatg tatgaacgta tgcttgataa aagcaataaa
     2401 ccctgtttgt ctcaaataat agaatatata ggtaaagatg gatatgcacg attgtgtaag
     2461 ttggaaactt acttgaaaac tcaatataat ttatcaagag aacttcgatt tccttttgga
     2521 aacaattatg gttggggtta caaatatagc cataaatctt ttcatttatt ttatgctttt
     2581 tttgaaaaag gtgcatttac tgttctgatt cagataggtg acaagcaagt tccagcagta
     2641 gaaaaaacgt tggtagcttt gacaccaaaa gctaaagaac tctgggtcac tcgttatcct
     2701 tgtggcaata atggaggctg gattaagtat cgtattttag aagatgcaga cttacttgat
     2761 attattcaat atataagagc aaaaaaatct ccacctaaac aaaattaact tttatttttt
     2821 gtgatttaaa tatagtgtat ataaattcca ttctttccat ataaaaaaaa taatataaaa
     2881 aatatctatt agtataactc atttagtatg tcttattcta ctttttacag ataattaaaa
     2941 agttttatca taaataaatc ataactagac atcctagtaa atcaaaatta atttattctt
     3001 ttttattcga atttttgata atagtcccta tacatatgcc aattatcatt ccaaaaatta
     3061 gaccataaca cattgtaata cctacatttc ctttagaaat acctatagca caacctatag
     3121 ccattccaag tatcatgcct ataaacatac atataataac taaattgttg tttttattca
     3181 tagtttttct ccattcaata tattttaaat tgcctagctc gccttcttag cttaaaacaa
     3241 agaaatataa attaactttc tttcattcta atatctgcct gtataaatgt caaacaacac
     3301 atttcttgtc ataacctcct ctttttgaag taagtacaat ctcccagacg caataatatc
     3361 aatttcctaa aaccatttaa gtaactttta tatttacata atatttacaa tcataaacta
     3421 tattgttatt catttataca tcataaacac tccctcctaa tcattattat acttaaaata
     3481 atgttcattt ataataatat atctaacgca ctttaattga aaaaactaaa actttgcctt
     3541 tcattcccat atataaataa gtatactttt tgatgctcta ttacaaatca actaaattaa
     3601 ttgatataaa ttatcatttt ctgttgaaaa taccattc
//
LOCUS       DAELJO010000296         3591 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.298, whole genome shotgun sequence.
ACCESSION   DAELJO010000296 DAELJO010000000
VERSION     DAELJO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.298"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            117..1745
                     /gene="aspD"
                     /locus_tag="KPB88_003723"
     CDS             117..1745
                     /gene="aspD"
                     /locus_tag="KPB88_003723"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG0055957.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1866..2861
                     /locus_tag="KPB88_003724"
     CDS             1866..2861
                     /locus_tag="KPB88_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG0055958.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3050..3433)
                     /locus_tag="KPB88_003725"
                     /pseudo
     CDS             complement(3050..3433)
                     /locus_tag="KPB88_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1266 a          498 c          558 g         1269 t
ORIGIN      
        1 ttaatttcat actttacttt ttctataaac ctattataat taaagtataa tcatttttgc
       61 aacttgttta ttttattata aaaatgtgaa acttattaca taaggaggaa tttaacatga
      121 aaaatcatac ttataaagaa atcaaaaaca tatatggaaa aataagccct tttgaattta
      181 aggacaaatt aatagatatt gcaaaataca ctgcaaaaga aaataacaga gaattacttg
      241 atgcaggtcg tggaaatcca aactggactt gttcaactgc tagagaagcc ttttttactt
      301 ttggtcattt cgctattact gaaactcgtt ctaattggga tttaggtcat ttggctggaa
      361 tgcctcaaaa aaagggtatt aaagaaagat tttttaaatt tataaatgaa aatatagata
      421 tgcctggagc atatttagct agagatatta taaactttgg tataaatgaa cttggatttg
      481 atggagatga atttgttcat gaactagctg atggaattat tggtgataac tatccacttc
      541 cagatagaat gttacctcat atggaaaaaa tagtacacga ttacctagtt caagaaatga
      601 aatatgatat atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa ggtgctacag
      661 ctgctatgtg ctatatattt gattcattaa tggcaaatga acttctaaaa aaaggagata
      721 caatagcctt aatgactcca atatttacac cttatcttga aatacctaac cttcctcgtt
      781 acgactttaa agtggtaaat ataaatgcta atgaagttga tgaaaaaggt gctcatacat
      841 ggcaatatac aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa gctttattcg
      901 ttgttaatcc taataatcct gcttctatag ctatggatga aacatcttgt aataatctaa
      961 tagatgtaat tgaaaattat aataaagatt taatgataat atcagatgat gtatatggaa
     1021 cttttgtaga agatttttca tccttaatgt caaaactacc ttacaacact gttggagtat
     1081 attcttattc aaaatatttt ggagttacag gatggagact tggtacattt gcccttcata
     1141 agaaaaatgt ttttgataag aaaataaatg atttaactgg agaattaaaa aaatcagtag
     1201 ataagcgtta cagtgatatg agtcttaacc cttctagcct ttcatttatg gaaagagttg
     1261 ttgctgatag cagacttgtt gctctcaacc atactgctgg tctttctact ccacagcaag
     1321 tacaaatggc gtttttctct gcttttgcat tgattgacaa agtagatgcc tacaaaaaac
     1381 tcaatatgag tatctgccat agacgtcaaa aactattatt tgaagcttta gagcttccaa
     1441 ttaatgaaaa caaaaacaat gctgcatatt acactcaatt tgatattgaa gaatgggcta
     1501 aattaaacta cggagaagac ttatttaaat ttataagtaa aactgcatct ccagtagatg
     1561 ttctttataa attagctaat gactattcag tagttttact tagtggaaat ggattctacg
     1621 gccctgaatg gtcaattaga atatctctag caaatctata tgatgaagct tatactaaga
     1681 taggaaaagc cataagagag atattaaatg gttatatcgt tgaatggcaa aaaattaaga
     1741 aataattata ttacactgaa atatctaaat tatatgaata aaagagttta tgtagacctt
     1801 aactactttt tttgatataa tcctatcgga attataaatt ttatttatga aaggcagaga
     1861 atagtatgag tattaagaaa aaagttgcca tagtttttac tggtggaact atatctatga
     1921 cagtagatga taaagtcgga gcagctatac caactttatc aggtgaacaa atactatcaa
     1981 tggttaccaa cattgataaa gttgcagatg ttgaagtttt taattttgat gaaattccag
     2041 gacctcacat aacaccttat agaatgatgg aattgaaaaa ctatgtaaat gatttattag
     2101 caagaaaaga tattacaggt gttgtaataa cacatggtac agatagttta gaggaaacag
     2161 cttattttct agaccttaca attgacaaca tcaaacctgt aatagtcaca ggtgctatga
     2221 gaagtagttc tgaacttgga tatgatggtt caagtaattt atctgcatca gtttgtactg
     2281 cagtatccaa agatgctatg ggaaaaggag tccttgtagt tttaaacaat gaagtattat
     2341 tagcttctga agctactaaa acaaatactc tttctctaaa tacatttaaa tctctaacta
     2401 gtggcccatt gggaataatt gactgcaacg aacttgtcct tacaagagat attgtaaata
     2461 gaacaattat agatactgat aaggtagaat ctaaagttgc cttatttaaa gcatatgtag
     2521 gagaaaatgc tgactttata agatttgctg ttgatagtgg ttataaaggt atagttattg
     2581 aggcaatggg cagaggtaat attcctcctc aaatgctttc tggagttgaa tatgctagag
     2641 aaaaaggact tcctgtagtt atagtttcta gatgtcattc aggtagagta ttcgatagtt
     2701 atggttactt tggttctggt agagatttaa aaaatcttgg ttgtatattt ggtggggact
     2761 tgccaggtca aaaagctaga ataaaactta tcttagcact tggaaaaact gataaccttg
     2821 atgaaatcaa agatttcttt gaaaaaggaa tctattgcta attctaataa cttctattta
     2881 attatttatt gatatttcaa catttttaaa tcaaaatcta atacttatgt cagttaaatg
     2941 tcagcaaaat attttatcca caatttttac acaagttttc cacaacttat ccccaatatt
     3001 atccacaaga aaaaaggtag cacttatatt agtagctacc ttttatttat taatcttcat
     3061 ctttccagct ttttatccaa tttcctattt ctattatttt ttcttcagtt atttcttctc
     3121 catcatgcaa ttttgaaatt agttctgata atggattgtt atgtagacct ccaaaaattc
     3181 tttttccctc tattttgaga tattctttct cttttattaa tactctatag tgtacatgtt
     3241 tcccaattct ctgcgatatg aaaaaacctt ttttagttaa tctagctaaa agtgtaattg
     3301 tagttgtatg cttccaagca tatgtttttt ctatttaatc tgcaacatct tttgatatta
     3361 cagttttata accagtattc catatgtatt tcataatttt taattctgca tctggtaatt
     3421 ttttattcaa cataatatat tccttctttc aatttatgtc acttcctcaa acttatctag
     3481 cttaaattaa taataggagc tttttatcac aaaaactaca ggtaaatttt tctatatatt
     3541 ctacgtattt agtctgtttt taagtgtgaa attacgttca tacctataaa t
//
LOCUS       DAELJO010000297         3492 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.299, whole genome shotgun sequence.
ACCESSION   DAELJO010000297 DAELJO010000000
VERSION     DAELJO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.299"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1804
                     /locus_tag="KPB88_003726"
     CDS             <1..1804
                     /locus_tag="KPB88_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055959.1"
                     /translation="FLNRTKTKNYLYQIFNVIINSTKLIFNYSWIYIFLSIIFSVFQG
                     FFPIISLLIMQKMINAIQMKNIGQDIFLKLIFIYIVLEMIDLMAVEIYNLYSTTFMKR
                     FTRHIEILMLEKANRLTTKDFENKEIYDLINRAQSQNGATVIDYFNSWILIFQQAITI
                     FSSIIIIISYQYWIVILIIIAPVIQFYYSQKLGHEQYEISMKRTSEERKCWYINFLML
                     LGNSIKETILYGLGNLFINQYGKLKEKFILQDYKIMKKTFEMNFIIIIIGQLFTGVIY
                     FHILLNGFWQKIYMGDVTTYITCITTIKNGIEVILSMMSTIYSKSLYMELLFRFFDIP
                     EKTELNKTKLKIDNIEKIELKHLYYKFTEDSGYVLKDINLELKKNSPIALVGRNGSGK
                     STLIKIILGIYDDYEGDILINGINLHNIEKENYYNKISCVFQDYTKYEASVKENVGYG
                     NLDQINNEDLIKESLIKSGLDINQLGSNKLNTILGYWFGENQLSEGQWQKIAISRAMM
                     KDADFYILDEPDAALDGIAENDMLLTYKKMFENKFGIFVSHKTHHLNLLCENIIVLNN
                     GQIAECGNHEELIEKEGIYHQLYSSQIISKLETN"
     gene            1833..3260
                     /gene="ccpM"
                     /locus_tag="KPB88_003727"
     CDS             1833..3260
                     /gene="ccpM"
                     /locus_tag="KPB88_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG0055960.1"
                     /translation="MEQKPFIHLFQTSTGFYFYDVNTDSILNIDENVYDYLKKEQLGE
                     SSSNTLEYVEFLRKQGYLKSNKVKITEHPETEVLKYHCENRVEGIILQVTQNCNLHCD
                     YCTYSGGYINRIHTNKRMSKETAKRGIDYLISHSRDCQYVSIGFYGGEPLLEFDLIKW
                     CIEYSKANIEGKNLKYNLTTNATLITEEIIELFEENNVSIMISLDGPAEIHDKNRKFA
                     NNNVGSFSTIMKKLDMISTKYPNYFKNNVHFNSVLATDNFSCVNKFFTKEELFQNSLF
                     LSSIVSEVNAKVKTEKSQSFYEESQYALFIGYLTLLGRIKKEDSSKLLQTQISTIGDT
                     RNGKQGKQRLVMPEKWHHGGPCVPGVMRLFINVDGYFYPCEKASEASDSMNIGNLDQG
                     YDLKKVEKLLNIERINGDKCHSCWAYENCRICAVSLSVDSTQEDIAKECEYIRSSIES
                     DFQDYCVLKELGFDFETKELIENTI"
     gene            3323..>3492
                     /locus_tag="KPB88_003728"
     CDS             3323..>3492
                     /locus_tag="KPB88_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG0055961.1"
                     /translation="MKKVLIYPFTEEMAPFVKQENLLKDIDITSIVSLKGNGILGDKY
                     KFNGKSLEVSEN"
BASE COUNT         1443 a          335 c          543 g         1171 t
ORIGIN      
        1 cttccttaat agaactaaaa ctaaaaatta tctttatcaa atttttaatg ttataataaa
       61 cagtactaaa ttaatcttta attatagctg gatatatatt tttttaagca ttattttttc
      121 agtatttcaa ggtttttttc caataatatc tttgttaata atgcaaaaaa tgataaatgc
      181 aattcagatg aaaaatatag ggcaagatat ctttttaaaa ttaattttta tctatatagt
      241 cttagagatg attgatttaa tggctgtaga aatttataat ttatactcta cgacatttat
      301 gaaaaggttt accagacata tagagatttt aatgttagaa aaagctaata gattaactac
      361 gaaggatttt gaaaataagg aaatttatga tttaatcaat agagctcaat ctcaaaatgg
      421 agctacagtt attgattact ttaactcctg gattttaatt tttcaacagg caatcacaat
      481 attttcctcg attattataa ttatatctta tcaatattgg attgtaatat taatcataat
      541 agccccagtt atacaatttt attactctca aaaattaggg catgagcagt atgaaataag
      601 tatgaaaaga acttctgaag aaagaaaatg ttggtatatt aattttttaa tgctattagg
      661 aaattcaatc aaagaaacaa ttttatatgg attaggaaat ttatttataa atcagtatgg
      721 aaaattaaaa gaaaaattca ttcttcaaga ttataaaata atgaaaaaaa catttgagat
      781 gaattttata attataatta ttggtcaatt atttacagga gttatatatt ttcatatact
      841 tcttaatggg ttttggcaga aaatatatat gggagatgta actacttaca taacctgtat
      901 aacaacaata aaaaatggta tagaagtgat attatcaatg atgagtacaa tatatagtaa
      961 atctctatat atggaactat tatttagatt ttttgatatt cctgagaaga cggaacttaa
     1021 taaaacaaaa cttaaaatag ataatataga aaaaatagag ctaaaacatt tatattataa
     1081 atttactgaa gattcaggtt atgttctaaa ggatattaat ctagaactaa aaaagaatag
     1141 tcctattgca cttgttggta gaaacggttc tggtaaatca actttaatca aaattatact
     1201 aggaatttat gatgattatg aaggagatat cttaataaat ggaattaacc tacataatat
     1261 cgaaaaagaa aattactaca ataaaataag ttgtgttttt caagactata ccaaatatga
     1321 agctagtgta aaagaaaatg taggatatgg aaacctagat cagataaata atgaggattt
     1381 gataaaagag tcattgataa aatctggact agatattaat caattgggga gtaataaatt
     1441 aaataccatt ttaggatatt ggtttggaga aaatcaactt tcagaaggcc aatggcagaa
     1501 aatagcaata tcaagagcta tgatgaaaga tgctgatttt tacattttag atgaaccaga
     1561 tgccgcttta gatggaatag ctgaaaatga tatgctttta acttataaaa aaatgtttga
     1621 aaataaattt ggtatattcg tatctcataa aacacatcat ttaaatttgt tatgtgaaaa
     1681 tataatagtt ttaaataatg gacaaattgc agaatgtgga aatcatgagg aattaatcga
     1741 aaaagaagga atttatcatc agttatattc aagtcaaatt ataagtaaac tagaaacaaa
     1801 ttaataatta ctttgaaaag gagttaagaa ttatggaaca aaaacctttt attcatttat
     1861 ttcaaacatc aactgggttc tatttttatg atgtgaatac tgattctata ttaaatatag
     1921 atgaaaatgt ctatgattat ctaaagaaag aacaacttgg agagtcaagt agtaatacat
     1981 tagaatatgt agaattttta agaaagcaag gatacttaaa atcaaataaa gtgaaaataa
     2041 cagaacatcc agaaactgaa gtattaaagt atcattgtga aaatagggta gaaggaataa
     2101 tattacaagt aactcaaaat tgtaacttac attgtgatta ttgtacttat tctggaggat
     2161 atataaatag aatacataca aacaaaagaa tgtctaaaga gactgctaaa agaggaattg
     2221 attatttaat aagccattca agagattgtc aatatgtgag tataggtttt tatggcggag
     2281 agccattact agaatttgat ttaattaaat ggtgtattga atattcaaaa gcgaatatag
     2341 aaggtaaaaa tttgaaatac aatttaacaa ctaatgcaac tcttataaca gaagaaatta
     2401 tagagttatt tgaagaaaat aatgttagta ttatgattag tctagatggt cctgctgaaa
     2461 tacatgataa aaatagaaag tttgcaaata ataatgtagg atcttttagt actataatga
     2521 aaaaactaga tatgatatct actaagtatc caaattattt taaaaataat gtacatttta
     2581 attctgtatt agcaactgat aacttctctt gcgttaataa attctttact aaagaagaat
     2641 tatttcaaaa ttcattattt ttatcttcaa ttgttagtga agtaaatgct aaagttaaaa
     2701 ctgagaaatc acaaagtttt tatgaagaaa gtcagtatgc tttattcata ggatatttaa
     2761 ctttacttgg tagaataaaa aaagaggata gttctaaatt attacaaact caaatctcta
     2821 ctattggaga tacgcgaaat ggtaaacagg gcaaacaacg tttagtaatg ccagaaaaat
     2881 ggcatcatgg gggtccatgt gtaccaggag ttatgagatt attcataaat gttgatggtt
     2941 atttttatcc atgtgaaaaa gcatcagaag catcagatag tatgaatata ggaaatttag
     3001 atcaaggata tgaccttaaa aaagtagaaa aattattgaa tattgaaaga ataaatggtg
     3061 ataaatgtca tagttgttgg gcttatgaaa actgcagaat ttgtgctgtt tctttaagtg
     3121 ttgattcaac acaagaagat atagcaaagg agtgtgaata tattagaagt agtatagaaa
     3181 gtgatttcca agattattgc gtattaaagg aattgggatt tgattttgaa acaaaagaac
     3241 ttatagaaaa taccatttaa tttaagatag catcttaaat tattacatta tgaaactaat
     3301 tagaatctat tggaggatta aaatgaaaaa agtattaatt tatccattca cagaggaaat
     3361 ggctccattt gttaaacaag agaatttact aaaagatata gatataacat caattgtaag
     3421 tttaaagggg aatggaatct taggagataa atataaattt aatggtaagt ctttagaagt
     3481 ttctgaaaat tt
//
LOCUS       DAELJO010000298         3461 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.300, whole genome shotgun sequence.
ACCESSION   DAELJO010000298 DAELJO010000000
VERSION     DAELJO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.300"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            323..1036
                     /locus_tag="KPB88_003729"
     CDS             323..1036
                     /locus_tag="KPB88_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055962.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1308..2846)
                     /locus_tag="KPB88_003730"
     CDS             complement(1308..2846)
                     /locus_tag="KPB88_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0055963.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENGQYLIKASDYQEQLIANNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         1203 a          463 c          488 g         1307 t
ORIGIN      
        1 agtttaaaag ggagttgaac ttaaagtttg attagaaaaa gtcaatttta agacaactcc
       61 ttttatttta ctattgtatt aatctgtatc tgtaagtgta ttagtctaat attataaact
      121 ttggatgaaa ttaattttct ataggtttta atgtatcata cactttattt taaagaaact
      181 tatcttaatt ttcatagtga ttgaatttgt ctattagaga tgttactatt agtaatgtaa
      241 aagcaagagt agtgaatata aaaaacaaga tagtgatact gcaagattag aatataataa
      301 atttttatgg agagatttca atatggatac atgttttaat aaaaaaatat tacttgttga
      361 tgatgaaaag gatatagtag atttaattga agaagtactc ataaatgatg gttttaaaaa
      421 cattattaaa gcttataatg gtctagatgc tatttcgctg tgtaagatag catgtccaga
      481 tgttgtcatt cttgatataa tgttaccaga tatagatggt atagaagtgt gtaaaaaaat
      541 tcgagagttt tcatactgtt ctattctttt tttatcatca aaaaatgatg atattgacaa
      601 aatactaggt cttagtagtg ggggagatga ctatataaca aagccattca gtccaagaga
      661 aattgctttt cgagttaaag cgcaattacg tagacaacag tatcaaagca ttgtaccatc
      721 agatagtgaa gttattaaaa taggagatat tactattgat atagaaggaa accgtgtata
      781 taaagataga aatgagattg agttaactgg aagggagtat catcttttaa gctacatggc
      841 tcaaaatgta aataaaatta taggaaaaga aagactctat gaacaagtat ggggagtata
      901 tagtagtatt tgtgacaata caattatggt gcatatacga catataagag aaaaaattga
      961 agataacccc tcaaatccta aaatacttat aacagttaaa ggactaggat ataaacttgt
     1021 gaatagaatc gattagaata gattttaata ttaatgaaga tatctcaagt tgaattttta
     1081 gttaatgagg cattctttta tgggtgaaat caaatgtatt ttcgttatct caacaatgaa
     1141 gaagaggtct atctctattt tgagatagcc tcattaagtt tatatcctgg taatgtactt
     1201 tatgttctaa taaaattttt ttagtttatt tgcagtgaaa cattaactag aacattaaaa
     1261 tttgcattga aataccaatt aaataatatt gtatattgtg tataaactta tactaattgt
     1321 ttttctatat taaatacttt atcaatccaa cctttataaa atttttcttc atgggaaact
     1381 aggattatac ttcctctaaa ctgtttcaaa gcattttgta aagcatcctt tgtctctgca
     1441 tcaaagtgat tggttggttc atccagaata agaaaattac aaggagataa catcatacaa
     1501 cataatttaa ccttagattg ttcaccacca cttaaagtag atacactcct tgaaacatgc
     1561 tcttccttca caccacatct ggctagatga cgtctaatct cttttgtatt taattttgga
     1621 tatttatctg ctactatttg aagtggggtt ttatcaggat tttcccattt taaatcttgc
     1681 tcataataac caattttaac ttgttctgaa aactggaaat ctccagatat acgaggaata
     1741 tcttttacta atgtctttaa caaggtagat ttaccgattc cattaaaacc agttataact
     1801 attttttgac ctccatcaac agaaaaattt aattttggaa gtaaagaata gtagtatcca
     1861 acttcaagat ttgttatagt aagagcattt tgagccgata tttcaatttc tgaaaattga
     1921 atatttggtt ttcctgtaaa actaggagga gctattcttt ctattttatc caattgtttt
     1981 tgtcttcctc ttgcaatttt tgaatttact ccagctttat ttttacgtat aaatgtttct
     2041 tctttttcta ttttcttttg ttgtgcttga taccttctaa tataatcttc tcttaaatgt
     2101 gatttttgtt taaggaactc tgaatatttt ccatgatatt tcttaatcat gccaaattct
     2161 atatcaagaa tacctgtaga aattttttct aaaaagtcaa aatcgtggga aactacgata
     2221 aatgccccat taaatgtatt taaataattt gagagccatt caacatgttc tttatctaaa
     2281 aagtttgttg gttcgtctaa taatagaaca tcatggttag aaagtagtaa ttttgctaaa
     2341 attacctttg ccctttgacc accactaagc tctccaacaa cacgattcat tcctattgaa
     2401 tctaatccaa gaccatttgc tattttattt atttcattat ctatagaata gaaattattt
     2461 gctattagtt gttcctgata atctgatgct ttgattagat attgcccatt ttcagatata
     2521 gcactttttt gatagagtag attcatttcc ttttctattt tataaagttc ctcaaatgca
     2581 gtgtgtaaat agagtgatat tgttgtatct ctattgattt ctgcatattg gtctaaatgg
     2641 cctatattga tattaggttg ccacttgatt tctcctgaat ctggaacaac ttctcccaat
     2701 aaaattttaa ttaatgtact tttacctgta ccattttggc caactacacc tatatgttca
     2761 cctttgaata aatcaaagtt agctttctca tacagtgatt tatccataaa acattgtgat
     2821 aaattagtta cttttaataa actcataatt caaactcctt ttcttttatt aaaaaattta
     2881 tggaaataaa aaaagcagag gaatggcatt gtaaaatgcc gacctctgct tttggaaaaa
     2941 caaaaggtca tagacaagca ttgttgtcta tgaccagaaa atctatatta tctatcggca
     3001 aataggtaat tttaaataag catagccaaa aaactatact aaacctatat ttgccgatga
     3061 agatagaaag ctttaagctt tcagattaac ctctacacaa cgcttcatcg tatacatttg
     3121 tgtagagtta agttttctaa caagtttaac taattctata taaatcataa ttattcacat
     3181 cctaatctta gtattaattc tagtttatct atatttaaat tgtacttttg tactttgtgg
     3241 attatcttat ttatttaaaa tacgttttat taattatagc ttaagttatg ttatcataat
     3301 acttagttat agtcaataag aaagttatat ttactattat tatattaatt tagtaaatct
     3361 atcattacaa agttatttga agactcgcta tcaataaaat tttatagtct ataagaaaat
     3421 taagattatg agttttataa ttttgatata gggatactat t
//
LOCUS       DAELJO010000299         3413 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.301, whole genome shotgun sequence.
ACCESSION   DAELJO010000299 DAELJO010000000
VERSION     DAELJO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.301"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..249
                     /locus_tag="KPB88_003731"
     CDS             <1..249
                     /locus_tag="KPB88_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HBG0055964.1"
                     /translation="NIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGITNDDIGVIEV
                     QDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            323..556
                     /locus_tag="KPB88_003732"
     CDS             323..556
                     /locus_tag="KPB88_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055965.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPSTRHVILHLNDCCKWKKYFRTKKKFYRS"
     gene            712..1476
                     /locus_tag="KPB88_003733"
     CDS             712..1476
                     /locus_tag="KPB88_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0055966.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(1761..>3413)
                     /locus_tag="KPB88_003734"
     CDS             complement(1761..>3413)
                     /locus_tag="KPB88_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0055967.1"
                     /translation="ILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIVDKSK
                     NNKIHKNKALYINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDY
                     QINGLNWFKVLDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKN
                     EFEKFAPDIKVLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCIL
                     DEAQNIKNPVALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKF
                     QELFINKEDNVKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYS
                     KDIQDKMKDKNLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSI
                     NENHKILLFSQFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVF
                     LISLKAGGTGLNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTI
                     EEKIIKLQESKKELINQFINGELSNEGVLKSLSDEEIINLFN"
BASE COUNT         1205 a          399 c          484 g         1325 t
ORIGIN      
        1 aatatagaaa gtgaagttgt taaattatat ttaaatgctg gtaagaagaa gaaaataaga
       61 gttttagata tagttggtgc atttagtaat ataaaaggta taactaatga tgatataggt
      121 gtaattgaag ttcaagattt atgttcatat gtagatatat taaattataa gggagatttg
      181 atattaaaaa aatataagga gatacctata aagaaaaaaa tggttaaagt gaaaagagat
      241 atacgataat ttagtcaata ttgatgaatt gctttgttat cgaaatgaag ttgcaaaaaa
      301 cacaatagaa gttgttaagg atttgaaagc tgaagatatg aaacgaaagg tctctccaat
      361 tgacttagaa aggattttgg tgactggagg agtaataaag catgaagatt ctatttggtt
      421 gttggacttt tgggggaata aagatattgc tggaattttg cttatgcctt ctactcgtca
      481 tgttatactt catttgaatg attgttgtaa atggaagaaa tattttcgta caaaaaagaa
      541 attttacaga agttaaatta taataaatta gacaaacaat agataaatta gcaaggaatg
      601 ttgattttat ctacattcta agttatacta tttttaagtg gctaatggaa tagtcatttt
      661 aaatataagt aataaatata tacctatagg gatattaaag gggggaaata tatggcatat
      721 tttaactata acgacaaaca gtgctactat aatgaaattg gggatggtac accattactt
      781 tttttacatg gaaatacagc ctcttccaag atgtttaatg atataatcga tttttataaa
      841 gatgaatata aagtaatatt aattgatttt gtaggtcatg gaaaatctca aatggtcgat
      901 aaattttctg ctgacttatg gtttgatgag gctatgcaag tgatatgttt tttagaagca
      961 atgaactata aaaaagtaaa cataattggt agtagtggag gagctcttgt tgcattaaat
     1021 gttgcacttg agagacctga tttagtgaat aaggtaattg cagatagttt tgaaggagaa
     1081 gttcctctag agtcatttgt acaaaatgtg aaaatagagc gtgaagcatc taagcaagat
     1141 gatggtgcaa aagcttttta tatttataat caaggagaaa actgggaaaa agttgtagat
     1201 aatgatacag aagctatttt tgaacattat aaaactatag gaaagttttt tcataagcct
     1261 ctagaaacta tgcaaccaga ggtgctttta acaggaagta gagaagatga gtttgtatct
     1321 ctaatatcta gtgatttttt tgaaaatact ttttcattac ttcttgaaaa gattaaaaat
     1381 ggtaaaatgt atttgtttga taaaggtggt catcctgcaa ttttatcgaa tggattagat
     1441 ttttcaaatg tagcaaagaa atttttagag gaataattta accaaaattt acaataatga
     1501 aaactgtaaa atggattgca aaacttacaa aagtgagtgt acatatactt cattattatg
     1561 atgaaatagt attgttaaaa caaaatattc taatagtcaa gttattacat ttgtatatgc
     1621 aatatgtata gaaagagata tcattagatt gtaaagttgg gattaaataa caatattaaa
     1681 ttttatttat tcagatattt tagcttaact atataaatgt tagaacaaat ttatatagtt
     1741 aagctgtagg attaaataat ttagttaaat aagtttatta tctcctcatc agatagactt
     1801 tttaatacac cttcattaga aagttctcca tttataaatt gatttataag ctctttttta
     1861 ctttcttgta attttattat tttttcttct atagtccctt tagcaatcaa ttttataact
     1921 tgtactgaat ttttttgacc aaatctatga gctctatcac tagcttgatt ttctactgat
     1981 ggattccacc atgggtcaaa gtgaattacc atgtcagcag atgtaagatt taaaccagtt
     2041 ccgcctgctt ttaaagatat taaaaaaact tttttatcca tactattatt gaactcatct
     2101 acaagttcaa gtctttcttt ggcattagtt ttaccatcta tataatggta tttaatttta
     2161 tacttatcaa gttccttact tatgttctta agcacagaag taaattgtga aaacaataat
     2221 attttgtggt tttcgtttat actatcccta agtatttcta aacatgtttc aatttttgaa
     2281 ctttttttat tgtaatcttt tactactata cttgggtcta aacaaagttg tcttagttta
     2341 gttaaataag aaaatataac tattttatct ttctttagat ttttatcttt cattttatct
     2401 tgtatatctt ttgaatatac actatatatc tttttctgct ctttattcaa ttcaacaaaa
     2461 aagttttttt caatcttatc tggaagttct ttcataactt gttttttact tcttctaaga
     2521 ataaaaggtt ttatcaattt tttcaggttt ttaacattat cttctttatt tataaacaat
     2581 tcctgaaact ttgctttact atatagatag ccaggcatta taaaatcaaa tatagaccat
     2641 aattctagta gattattttc cataggtgta ccagtgagtg caaatttatt ttctgcattt
     2701 atacttttta cagactcagt tactaaagct actggatttt ttatattttg agcttcgtct
     2761 aatatacaat aatcaaactt tatttcacta tatttttcta aatcatttct aagagtacca
     2821 tatgttgtaa gtattacatc aaaattttct aattccataa aacatttttc tctatctctt
     2881 ttatttccat gtatcaataa aacttttata tctggagcaa acttttcaaa ttcatttttc
     2941 caattataaa tgagtgatgt cggtgtaaca ataagacttt ttttattaga taatgataat
     3001 aaaaatgcaa tagtctgtat tgttttacca agacccattt catcagctaa tataccacca
     3061 aatttataat aatctaaaac tttaaaccaa ttgaggccat ttatttgata gtcacgaagg
     3121 tttgcattta aatttgcggg aatttctaaa tttatgctat caaaatttct aaatttttca
     3181 catatactgt ccagttcttt tttaccatct ataaacttta aatttttact acgtattaca
     3241 tcatttatat atagagcttt atttttatga attttattat tttttgattt atccactata
     3301 tctagatttt caactaattc aaaaaaatct tttgtttctc tttctgataa atctataaag
     3361 tttccatctt taagtttata aaaagtacgt ttttcttgaa aagctttcaa aat
//
LOCUS       DAELJO010000300         3354 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.302, whole genome shotgun sequence.
ACCESSION   DAELJO010000300 DAELJO010000000
VERSION     DAELJO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.302"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(83..448)
                     /locus_tag="KPB88_003735"
                     /pseudo
     CDS             complement(83..448)
                     /locus_tag="KPB88_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(469..1548)
                     /locus_tag="KPB88_003736"
     CDS             complement(469..1548)
                     /locus_tag="KPB88_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG0055968.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1602..2891)
                     /locus_tag="KPB88_003737"
     CDS             complement(1602..2891)
                     /locus_tag="KPB88_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0055969.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3054..3265)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1107 a          675 c          369 g         1203 t
ORIGIN      
        1 ctacatatag gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac
       61 gagtgtgcgt agctattttt taatcaaact catatccacc agataccaat attgaatcta
      121 attctatttt ttctcttaag tcttctggcc aatctataaa tggaagaatg gcagtagtgg
      181 ctggctttcc tattcttatc atagccacat tattaagtct ttttctttct acaatagcat
      241 ttgtacaatg agttgtacca agcatagcat attttattaa tgaatggtca atcttacttt
      301 catctataac tttttttaag gcattgtaaa caccagtttc aacatctttt gtagttgcca
      361 ccttaactga atgaattact tttaattccc catccagtat acaagcatct gtatttgttc
      421 ctccaacgtc aattcctaca cgatacatca aaatttcctc ctaaactttt attttccttg
      481 taattcttct actgttttat attccacatc atatccaaag tatccaggtc caactgtctc
      541 aattcctttt tctgttctcc acttttcatt acaaggaaaa gctactacca taacacgaga
      601 gccatatttt attctctctg tagttattgg aaatccagtt tctaaatcta atactgctat
      661 taaatctggt gtaatacaaa gtggtgtatc accttttttg gcaattaaat tctcattttg
      721 gaataatata gaatactctc catcatactc attaatccca gcaaaaaagg catgtcctct
      781 agtaaatcca cctttcaact ctctcttaat atcaattact tttccttcaa ataacttgta
      841 tccacgcaat tcttttagta tattctcaac agcacctttt gaatcagatt tattttcaag
      901 taaaaaccct cctatagttt cagctattgt aagagtatta cctatagcag cttttttaac
      961 ttgagctcca cttaaattat agtcactaat agaagaactt cctcccattt ctacagttaa
     1021 agttctagct aatctttctg accaaattcc atccacagga taaaggatgg cagaattacc
     1081 tttttcatca gacattacgg caggagatgc ctcatatcct tcaagataaa aagttaccat
     1141 ttgagcttca gggaaagctc tacccataga atctgcatct aagataggaa gtcctttttt
     1201 agcagcaact gctataggca taagtgagtt tatccctcct atttctatag gaattataac
     1261 atccactttt ctatttaata atttctccat tatttctaaa ggggtagtta actctacttc
     1321 tgaaagtaat ttttctattg taattacagg agaacccata ccagatactg gaactaccaa
     1381 agcatcatca tcaagttcat ctaaacttat tacttttaca ggaccatact ttttaatggc
     1441 ttgttttgca accaatttac ctacatatgg gtcacctcca cctccagttc caagcactgc
     1501 tgcaccaact gctattttat ctacactttc ttctgttaaa aatctcatgt tactttctcc
     1561 tcatatttct aatttatttt atactttata aacaaatttc tttatactgc tttgtctatt
     1621 tcttgcttat tttttgttga acaaagcact tttccaataa tagattgtac tacaaaccct
     1681 gctataaaac catctaaagg tgctatagta gtaagagtta taaatccata tgttgaaagg
     1741 taagttatta atataccaat tatccaagac actattgaac gtttaactat tggtttatat
     1801 aatactcctt tatcaaagcc tttaagttct tgctttacta tgtaatattc tgctgtatat
     1861 acaccaccaa caggtgcaat tacaattcca agtaaattta aaaatcctat aaaaccttca
     1921 tatattccaa atacagccaa taatgttgct ataataccaa ggactatagt taatactttc
     1981 tcaggagcct tcttaagtac aagagctata cttaatgatg ctgaatatac attacttgta
     2041 tttgtagtcc attgtgctaa tgtaagtact attatacctg gtatagcaat tcctaatgta
     2101 ataaaaattc tcataatatc tgaagttccc atacatttta ctaatactat cgaaactatt
     2161 atcataaaag catttccaaa ttgtattccc aaaaagcttg aaagagcaca gtctcttcta
     2221 cttttagccc aacgacttat atcaggagat acaattgcac caactattat tatactcatt
     2281 gacagagaaa ctccacctgc aaatgtcata gtagatttca tattatcatc aactgaaatt
     2341 ccatttgctc taaaagctaa gaatatagtt agcatcataa gaactaataa aaatggtact
     2401 gaatacacac tcaacttctc cattgcctta tatccataaa ttgcagtact catcatcaag
     2461 agaccaccta ttaggcttaa aatctgcata ctgacatcta aattaaatat atcttttatt
     2521 attatctgtg cattttgagc aaagaaacct acttgaactc caaaccaacc taatagagaa
     2581 acccctataa ctagagaaaa tatttttgct ccagcttctc caaatacata ctttgttatc
     2641 atagatgtag ataaacttgt agatgaacca accaaagagc atgtagtata caaaattgca
     2701 gaaaaaaatg aaccaacaat tactgacaat attgcttgtt tcattggcat actctgtgcc
     2761 aactcagcgc ctaaaataac agatgataaa tccacaccta ttgctactaa aactgaaaac
     2821 atagctaccc aaccacgttt ttgattctct ggaatctgtt ccagagcata gttaattttt
     2881 tcttcttcca ttttcatccc tccatttaaa tcgtaaaact actgatctta acatatttta
     2941 caattttcta atttataaat ttattaacta tcatattttt ataattttca tttaatatat
     3001 agttttaaat tttctgaaat aagtttataa taaaaaacgc ctcttggaaa aatccaagag
     3061 acgaatataa atccgtgtta ccactcttat tagtaaaaat taattttact cactcatttt
     3121 cttaaggcga attaaaccac tattctaaca taattacaat agtattctcc aaagtccttt
     3181 tcacataaat tccaatatta gttctcagcc aaatctaact ctctgtaaaa ttcctttatg
     3241 ctactttctt cttctacgaa tatataatat tgatttgtag ttaatttatg atttatatca
     3301 taatatatat gcaatatttt gtcaacttaa aaagtcaatt acttgcattt ttta
//
LOCUS       DAELJO010000301         3341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.303, whole genome shotgun sequence.
ACCESSION   DAELJO010000301 DAELJO010000000
VERSION     DAELJO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.303"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     misc_binding    182..444
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            520..3186
                     /locus_tag="KPB88_003738"
     CDS             520..3186
                     /locus_tag="KPB88_003738"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG0055970.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEDEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1354 a          364 c          630 g          993 t
ORIGIN      
        1 aagcattaaa attaaaataa tcttgcaatt attgacatta taagacagaa taactgtaaa
       61 aacatatttt aaaatataac aactatataa aagagaatag acataattaa gaaaattcag
      121 aaaaaataaa ataaaaataa ttgacaaaga gaataatatt tgtatataat aatatgtaat
      181 attaaagact gagaagaaga ggagtaagta aatactacta aaagagagga aaattcactg
      241 gctgtaagat tttctaagta taagatttac tgaatgtagc tttggagttt ctaaactgaa
      301 ctataagtaa gtttagacgg ttagaaccgt taaattcttt gagagtcaaa gttgataaaa
      361 taacattaac caataattta attatatgtt aatgtatttt aagctttgga attaaggtgg
      421 taacgcgagc ttttcgtcct ttttaaagag gatgaagagc tcttttttat ttctaaagat
      481 atttaaaaaa tttatttata aaatacagga ggcaggacaa tggaaaatac gaatttatca
      541 aaaacttata acccaaaaga ttttgaggct aggctatata aaaaatggat ggatgaaggg
      601 tattttaaat ctaaaccaaa tccagataaa aaaccattta caataatgat gccaccacca
      661 aatataacag ggcaattgca tatgggacac gctcttgacc atacacttca agatatatta
      721 atcagatgga aacgtatgga tggatatgaa gctttttggc ttccaggaac tgaccatgct
      781 tctatagcaa cagaagttaa agttgttgaa agaataaaaa aacaagaagg caagacaaag
      841 tatgaaatag gtagagaaga atttttaaag agagcctggg aatggaaaga tgaatttgga
      901 ggaaaaatat ctaatcagct taaacaacta ggtgattctt gtgattggga taaagaaaga
      961 tttactatgg atgaagggtg taatgaagca gttatagaat tttttgtaag cctatatgaa
     1021 aaaggtcaca tatacagagg aaatagaata ataaactggt gcccagattg caaaactact
     1081 ttatcagatg cagaagttga acatgaagaa cacgacggaa acttttatca tataaagtac
     1141 ccactaaaag atagtgaaga ctttttagaa atagctacaa ctagacctga gacaatgata
     1201 ggagatactg gtatagcagt aaatccagaa gatgatagat ataaacattt aataggaaaa
     1261 acagcaatac ttccattagt aggaagagaa cttccaatag ttgcagatag ttatgttgat
     1321 ttagagtttg gaactggtgc agttaagatg actccagcac atgaccctaa tgattttgaa
     1381 gtaggattaa gacataatct tgagcagtta aatactatga atgaagatgg aactatgaat
     1441 gaagtttgtg gaaaatatga aggtatggat agatttgagt gtagaaaagc tatcgtagca
     1501 gacttaaaag aacaaggtta cctaattaag ataaaagagc ataatcataa tgtaggaact
     1561 tgttatagat gccatacagt agttgagcct agattatcag agcaatggtt tgttaagatg
     1621 gaagaacttg caaaaccagc tatagatatt cttaaaaaag gagaattaga atttgttcca
     1681 gataaatttg ataaaactta tttacaatgg ctagaaaata taagagattg gtgtatatca
     1741 agacaattat ggtggggtca tcaaatacca gcttattatt gtcaagagtg tggagaaata
     1801 gtagttgcta gaaaaatgcc agataagtgt cctaaatgtg gaagcactca ttttaaacaa
     1861 gatgaagatg ccttagatac ttggttcagt tctgcattat ggcctttctc tactctagga
     1921 tggccaaata agactgaagc tttagattat tattatccaa caagtgtact tgttacagga
     1981 tacgatataa tattcttctg ggtagttaga atggcatttg caggtatgtt ctgtatgaat
     2041 gaaaagccat ttgaccatgt tttggttcat ggattagtaa gagattctca aggtagaaaa
     2101 atgagtaaat ctttaggaaa tggtatcgac ccattggaga taatcgagca atatggagct
     2161 gatgccctaa gatttacatt aacaactgga aattccccag gaaatgatat gagattctat
     2221 atggaaagag tagaatttgc tagaaacttt gcaaataagt tgtggaatgc atcaagattt
     2281 gtatttatga atattgatga agatataatt aaaaacatga ctagagaaag tgttaaagag
     2341 gacttaactc tagctgataa atggataata tcaagagcaa ataatattgt aaaagaagca
     2401 actaataaca tggataagtt tgatttagga atagcacttc aaaagatata cgattttact
     2461 tggtcagaat actgtgactg gtatattgaa atggttaaac ctagattata tggagaagat
     2521 gcaaatgcta aatctgcagc attatataca ttaacttatg ttcttgaaaa aatattaaaa
     2581 ttattacatc catacatgcc atttataaca gaggaaatat atactcatct tcctactgtt
     2641 gaaggatgta taatagtatc tgaatggcca aaatataatg aagaagataa catggctgaa
     2701 gaagaagata tgatgaactt attgatggaa ggtataagaa gtataagaaa tgtgagagca
     2761 gaaatgaatg taccaccttc taagaaagct aaattgataa taatcccaag tgaagaaaaa
     2821 atagaagcta ttgagcttgg taaagactat tttataactt tagcatctgc ttcaaatgta
     2881 gagatagcaa aagataaatc taatgttcca gaagatgcag ttggagtagt tattgatggt
     2941 gttgaaatat ttatacctct taatgaatta gttgattttg aaaaagaaat tgagagatta
     3001 tctaaagaaa agaaaaagtt agaagatgaa ataaagagag ttaatggaaa gcttgcaaat
     3061 caaggtttct tagcaaaagc tcctgaaagt ttaattgaag aagaaaaagc aaaaaaagaa
     3121 aaatttgaag agatgattaa atctgttgag gaaagattaa caaatttaga atctaaaata
     3181 aaataaatat aagaattagt aaaaacatac aaaaagtata tattctatac tttttgtatg
     3241 tttttcttta tatatataca aatatgatgt tataattctt tacagatgtg ttatattttt
     3301 attttatgtt gtgaatatat gaattttatg tcttaaaatt t
//
LOCUS       DAELJO010000302         3331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.304, whole genome shotgun sequence.
ACCESSION   DAELJO010000302 DAELJO010000000
VERSION     DAELJO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.304"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..754
                     /locus_tag="KPB88_003739"
     CDS             <1..754
                     /locus_tag="KPB88_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0055971.1"
                     /translation="VLVSVWIAITIFYLVYTLLKYIKLKNMINDLSYDVEDDKIKNLY
                     ENLLKELKINKKIRLKYSYEVESPVFFNSCVILPPCDYTLKELNWIFRHELMHFKSKD
                     LYIKYLVIFLKCVYWFNPFVYIMEKFIDLDCEFYCDERVLKNCTIEEKQDYALTIISA
                     MRKGSNSSNKFVAGLHKESDIKKRVFSMFNEKYRNGILIAVILCLLSSITYFKIDLIT
                     TKSIFYPLSKQTPIENRTNSKPTYQVNVYIDN"
     gene            1106..1675
                     /locus_tag="KPB88_003740"
     CDS             1106..1675
                     /locus_tag="KPB88_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0055972.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDIAKEYEKLKLNLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(2012..3184)
                     /locus_tag="KPB88_003741"
     CDS             complement(2012..3184)
                     /locus_tag="KPB88_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0055973.1"
                     /translation="MIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGINFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWLLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT         1291 a          339 c          497 g         1204 t
ORIGIN      
        1 tgttttagtc tctgtttgga ttgctattac gatattttat ttagtttata ctttattgaa
       61 atatattaag cttaaaaata tgataaatga tttgtcttat gatgttgagg atgataagat
      121 aaaaaatctt tatgaaaatt tattaaaaga gcttaagata aataaaaaaa tacggcttaa
      181 atattcatat gaagttgaaa gtccagtatt ttttaattcg tgtgtcattt taccaccatg
      241 tgattataca ttaaaagagt taaactggat atttaggcat gagttgatgc attttaagag
      301 taaagacctt tatattaagt atttggtgat atttttaaaa tgtgtttatt ggtttaatcc
      361 ttttgtgtat ataatggaga agtttataga tttggattgt gaattttatt gtgatgaaag
      421 agttttgaag aattgtacga tagaagagaa gcaggattat gcattgacaa taattagtgc
      481 aatgagaaaa ggttcaaata gttcaaataa atttgtggct ggattacata aggagtcaga
      541 tattaaaaaa agagtgttta gtatgtttaa tgaaaagtac agaaatggta ttttgatagc
      601 agttatttta tgtttattat cttctattac atattttaaa attgatttaa ttactactaa
      661 atcaatattt tatccacttt caaaacaaac acctattgaa aacagaacta attcaaaacc
      721 tacatatcag gttaatgttt atatagataa ttaaaaaata caaagtttaa caagagatta
      781 atataaataa ttttaataag tatctttcac atatgttaaa tttactttaa ttatattaaa
      841 gccaatgaat tattctttag aagctccaat gccagtaaat acaggtaaag aaaaaaatac
      901 tataattgta gattaaaaat gttctaatat ttaaagaaat gcaaaagtgt aagaagggta
      961 aaaatctgtt ataatacatt aaaaactaaa tttagaaaaa tttacataat tagaaaaatg
     1021 atttgataaa aaataaagtt aaatattaac taagtaaatt tgaaaaagaa aaaatataaa
     1081 gtacaaattt aaaaggaggt aactaatgaa aaagaagttg gaagatatga ctttagagga
     1141 gttatggaac ttgtttccta tatttttgac tactcacaaa gaatgttgga aaaattggtt
     1201 taaagaagaa caatatagac taactggaat attgccaatg caaaaaattg ttagaataag
     1261 tcatattgga agtacttctg tgagtacgat ttatgccaag cccattgttg acatactgat
     1321 tgagataatt cctgattata atatgaaaga tattaaagat ttgattgagt cgaatggata
     1381 tatttgtatg tctgagagca gtagtaggct ttcacttaat aaagggtata cagtagatgg
     1441 gtttgaagaa cgagtttttc atttgcatat aaggtatgta ggtgataatg atgagttgtt
     1501 ttttagagat tacttaattg accacccaga tattgcaaag gagtatgaaa aacttaagtt
     1561 gaacctttgg aagaagtatg agcataatcg tgatgcttat acagatagta aactagattt
     1621 tgttagggag tttactgaaa aggcaaaggt gttgtattct aatagatata attagaaata
     1681 ggtacaatta gatatttatc ttatatatcg tattatattt tttaagaaag taattaatag
     1741 aaaaaggggt tgtttcaaaa tggagagata aatccctttt tcattgttga aatgataaaa
     1801 atatatttga tttcatgtaa aaaagagtgc ctcatcaact aaaaagttta ttttgagaca
     1861 cacacatttt tattaaggta aataaaaata tcacatttga ttaaatttta agttaatgaa
     1921 tatatgtgta aatatttaat tataagttat tataatttta catagtataa aacaatctaa
     1981 tttgtattat ataagataat ctaattttag actaaaataa tctaatataa agaaataaag
     2041 aaaattcact ctcagtataa gaaatatcca attttccatt gtacttatta acaatttctc
     2101 taatattctc aataccatat ccgtgagaat tactatcttt tttatctgaa ataaatagtt
     2161 cttgttttct attgatagga tttaccttgt tgtttgtcaa taaatagcta aaatactcct
     2221 tctcacaacg tgcctttaat accacttttc tatttgacaa atcaggaatc ttcaaagagg
     2281 cttctatagc gttatctaaa gaatttgaaa aaatagtaca taaatctata ggttctatac
     2341 ccaagtgatt gtctatatct atattaaagt gcacatcaac accattttct tctaaacttg
     2401 tatgacgaag atttaaaatt gagttaagag cttggttttt gcaaaattgt tttccactaa
     2461 gagtacctat acctattttt aactgattaa aatacttttt agctccagca atatcttcaa
     2521 tttctaaata cgattcaatc atttgaaagt gattattcat atcatgtcgc attttgcgta
     2581 tttgaatttg ttgattttct agtaagttat agtatttttc ttttaggtga tttgcttgtt
     2641 tttctatctt catctttaca ttttcaatca atggctgtaa aagaagtatc acaccgatat
     2701 ttgccagaat tgctgacaag atgattagat aagtgccata aggtaagttg cctaagataa
     2761 tattcttatt actacccata gggattaaat tagtaggtgg cagaataata gcaattaata
     2821 ttgataaaaa tgaagatata cataccatat caataaaaat ccatatttta tttgataagt
     2881 attgttttat attgaccaat ctattcttta ggaaaatatg tattaaaagc caaaataata
     2941 atttaattag agcagaaatt atctggacta ttgaaagtat taaaatatac tcttttgagt
     3001 gtataagttc ctcatatata gggttgttta taaaaagaaa attaatccca attataatag
     3061 gataaagtat catgacaact gatattatag gaattaattt gcttttgcta catattataa
     3121 gtagtataaa atatccgaat agtgtaaaca aaatatttat ggggtcggaa agccatatta
     3181 tcatttgact aataatagag tagcccaaaa tcaatatgat tttgaaaatc caatgattcc
     3241 taaagtctat aaaataggaa gtcaacttat aaagaaaaaa ggctatgaaa atattgatta
     3301 atattagaat aaaatttatg cttgtagaga t
//
LOCUS       DAELJO010000303         3263 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.305, whole genome shotgun sequence.
ACCESSION   DAELJO010000303 DAELJO010000000
VERSION     DAELJO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.305"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            334..2976
                     /gene="adhE"
                     /locus_tag="KPB88_003742"
                     /gene_synonym="adhC"
     CDS             334..2976
                     /gene="adhE"
                     /locus_tag="KPB88_003742"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0055974.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1269 a          406 c          619 g          969 t
ORIGIN      
        1 aaataacata taaaatttaa taagtaaaaa aaatgaaaga tataaattat cattttagta
       61 ttttatttag tcgaagtaaa ttattcaaat gaaatatatc tcatttagat aaaaaatttt
      121 agtaaaagta aaaaaaataa tatatttgtt aaaaaataaa ttgacaaaat attcagaaaa
      181 aaacttcttg acctaagaga gtaaaataaa taaaatctaa ggtatagaga tgaaatctat
      241 aaaattattt taagtataag ttaaaaatat agatgatgca tttaagattt ataaaaatta
      301 gagatgcata attattttag ggaggttttt agtatggaga aaaaagaaaa ggtagtagaa
      361 aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca gtgttgaaac attaagaatg
      421 agattagaag aaattagatt ggcacaaaaa gaatttgcaa catttacgca agaacaggta
      481 gataagattt tcttagctgc ttcaactgca gctaatcaac aaagaatacc tctagcaaaa
      541 atggcagtgg aagagactgg tatgggtatt gttgaagata aggttataaa aaaccatttt
      601 gcctcagaat atatctataa tgcttataaa gatactaaaa cttgtggtgt catagaaaaa
      661 gatgaagcat ttggttttac aagaattgct gaaccagtag gtgttttatc agcagttata
      721 ccaacaacta atcctacatc aactgcaata ttcaaatccc taatagcact aaaaactaga
      781 aatggtataa ttttctctcc tcatccaaga gctaaaaact gtactataga agcagctaga
      841 gttgtacatg atgcagcagt taaggcagga gcaccaaaag gacttatagg ttgggtagat
      901 gttccttcga ttgaattaac taatgttgtc atggcagagg cagaccttat attagctact
      961 ggtggacctg gaatggttaa atctgcatac tcttcaggta aaccagctgt tggagttggg
     1021 cctggaaatg tacctgcaat aatagatgag agtgcagata taaaaatggc tgtaagttca
     1081 atcttagtat caaaatcatt cgataatggt atgatatgtg cttccgagca agctgtaata
     1141 gtaccagaaa aaatatatga agaagttaaa aaagaattta aatatcgtgg agcacatttc
     1201 ctaaataaag aagaaactga aaaagtagga aaagtagtaa taattgatgg ttcattaaat
     1261 gccagaatag ttggacaacc agcacatgta atagccaaaa tggcggatgt agaagttcca
     1321 aaaacagcta gaataataat tggtgaagta gaatctgttg aattaaatga accttttgca
     1381 catgaaaaac tttctcctgt acttgcaatg tacaagtcta aaagttttga agatgcagtt
     1441 gctaaagcag aaaaattagt tgcagatgga ggctatggtc atacatcttc attatatgca
     1501 gactctataa atcatccaga gagagtagaa aagtttgtaa atgctatgaa gacttgtaga
     1561 gtattagtta atactccatc atcacaaggt ggtataggtg atttatataa ctttaaattg
     1621 gcaccgtctc ttactcttgg ttgtggttct tggggtggaa actcagtatc tgaaaacgtt
     1681 ggagtaaaac atttgcttaa tatcaagaca gtagctgaaa ggagagagaa tatgctttgg
     1741 ttcagagcac cagaaaaggt ttatttcaaa aaaggatgtt taggtgtagc agcaagagaa
     1801 tttaaggatg ttatggataa aaagaaagca tttatagtaa cagattcttt cctatataat
     1861 aatggatata ctaagaaatt aactgattta ttagatgaga tgggaattaa gcatactaca
     1921 ttctttgatg tagctccaga cccaacatta gcttgtgcaa gagaaggagc taaagctatg
     1981 gctgatttcc aaccagattt aataatagct gtaggtggag gaagtgcaat ggatgctggt
     2041 aagataatgt gggtaatgta tgagcatcca gaagtagatt tccaagactt agctatgaga
     2101 tttatggata taagaaaaag agtatatgta tttcctaaga tgggtgaaaa agcgtatttt
     2161 gctgctatcc caacttcagc aggaactggt tcagaagtaa ctccatttgc agttataact
     2221 gaccaagatt caggtgtaaa gtacccatta gcagattatg aattaatgcc aaacatggct
     2281 ataattgatg ctgatatgat gatggagatg ccaccaagat taacagcagc atcaggtgtt
     2341 gatgctttaa cacatgcatt agaagcatat gtatctatgt taagaactga accagctgac
     2401 ggtctagcac tacaagcagg taaaataata tttgaatatt tacctcgtgc atataaaaat
     2461 ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg catctactat ggcaggaatg
     2521 tcttttgcaa atgccttctt aggaatatgt cactctttag cacataaatt aggtgcattc
     2581 caccatgttc aacacggtgt tgcaaatgca ttattaataa atgaagtaat caagttcaat
     2641 tgtgcagaag ctccaaataa aatgggtgca ttctcacaat acagatatcc tgattgcata
     2701 caaagatatg ctgaatttgc ttcttttgca ggtattaaag gaagtacaga ccaagaaaaa
     2761 gtagataatc ttataaaagc aatagatgaa ctaaaggcaa aagtaggtct tcctaagact
     2821 ataaaagagg ctggagtaga agaatctaaa ttcttagaaa gattggatgc tatggttgaa
     2881 caagcttttg atgaccaatg tacaggtgct aatcctagat acccattgat gagtgaatta
     2941 aaagagatat atctaaaagt ttattatggt aaataatatc tattgtgcaa cataggttct
     3001 attgggtttg tatataaatt attagatttg aatataaata gagttttatt agagacacgt
     3061 agaagtaact ataaataata gattataatt taaattctaa atggaaatga taaaaagtca
     3121 gtaatttaat ggtaatttcg aatatgtgta tttaatcaat taactttaaa attagtcgga
     3181 aaattttttt attacatata attttaaagc ataatatata aaaaacttta tgaaaaaatt
     3241 ataaaaatat tcagaaaatt cta
//
LOCUS       DAELJO010000304         3230 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.306, whole genome shotgun sequence.
ACCESSION   DAELJO010000304 DAELJO010000000
VERSION     DAELJO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.306"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            50..748
                     /locus_tag="KPB88_003743"
     CDS             50..748
                     /locus_tag="KPB88_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055975.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            926..1804
                     /locus_tag="KPB88_003744"
     CDS             926..1804
                     /locus_tag="KPB88_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055976.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVELRADG
                     KYYYINKGMEGLGFYAVALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLTVIRN
                     FLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASSFHLL
                     TRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDATREM
                     TTYYDAKALSILGFNQ"
     gene            complement(2206..3162)
                     /locus_tag="KPB88_003745"
     CDS             complement(2206..3162)
                     /locus_tag="KPB88_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0055977.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1259 a          373 c          482 g         1116 t
ORIGIN      
        1 gttaagttgg tttttataaa cataaattta ttttagagag gtgaaataaa tgaaaatatc
       61 tagtcaatat agaagtcaat attcatttag atatgaaagt aatataaata atacaagaat
      121 aaatgaaagt atggttaaga aaaatgaaac tgtaggaaaa gacacttatt tatctaatat
      181 tatgaaacaa aagcaagaac ttaatgatag aattagagat ttaaaatata gacaagaggt
      241 ttatactaaa aaaataaatg acgcaattaa gaacttatgt aaatcagaaa taagagaaac
      301 aactaataac ttttctaata tagaaatagg tattaaaaat agcattatag aagataaaaa
      361 taaaagtaca atgttagatg aaaattcaac ttatctaaat acaaatgatg aaaaagaatc
      421 tttaatcact aaagagtcta atgaaaaaat tgaagaagaa atattaaatg atgaaaaatt
      481 agaagagtta gaacaaaaaa aggattataa agaggattct aataaaaaag agaaagtatc
      541 tgaggactta tctttagtag gtaaaactcg tgaagagctt gaaaatatgc ttaaaaattt
      601 tataaattta acacaagaag aaataatgaa acttgagtcg agaatagaaa agttagataa
      661 aaatgctgaa gaatacaaac aaaattcaaa gactaatata tttgataaaa cagatgaaca
      721 aaaaaaacat ataaatgtac tgatttaaac aatatgtttt aaaaaaataa gtttattata
      781 taatgaaaat ataaaggtac aatttcaata cacttctatt aaaccaataa tttatgaatc
      841 agatttaatt tcaatttact aataaccata tatttatgag ttaataattt tatgttttaa
      901 aaagaattaa ataaggggga gtagtatgaa gaagaatata ttaactacta tgattaccat
      961 gattattata ttgatgatga gtatgacagt aatggcagag ccatatcaag aagatgaaac
     1021 atctagcttg aaaaaacaga gtgcaaatga acgtaatcag actaatatag gcacaatgca
     1081 aacaaatgca ataagtaaag gtgctaatta tgaccctaaa tatcctctta aaggaatgtt
     1141 aacacaactg ggtcttaata caacaggtaa agagggatgg gatttactct atggaggaaa
     1201 tcccaatgga gttgagcttc gagcagatgg taaatattat tatataaata agggtatgga
     1261 agggctagga ttttatgcgg tagcattgtc aggtggctca tatcagagaa taggctttgg
     1321 aaaacttgac cctatagaag taatagctga tggaaattgg atttcttaca aacaagctca
     1381 agatacactt acagtaatca ggaatttttt aaatagcttt gactggagaa atgcatctga
     1441 gatggaaaga gctaatagag ctgcaaaatt ggtaaccgaa gcaaaatatg cagactctaa
     1501 atattgtaac atagtctatg gtaatctggt tgataaacgt ggtgtatgtg gaagttttgc
     1561 aagtagcttt catttattaa caaggttaat gggaatggat tcactatcta tattaaatcc
     1621 atcattaaat catgcttgga actatataca aattgatggg aaatggtata ggtttgatgg
     1681 gtctgaaata tcagcttttg gtggatcatt agactttgat taccgtaaat taaaggatgc
     1741 aacaagagag atgactactt actatgatgc taaggcattg tctatattag gatttaatca
     1801 ataaaaatag ggggaggctt cttatagagc ctttttttat tgatttgaaa tttaataagg
     1861 aattttatat gaaaatttaa tcaataaaag caaaaaaggc tctttataaa gcctttctaa
     1921 gtgttttttt aagtaaaatt ttttatatca acatcagttt taatcaaaac atataacatt
     1981 ttggacttac ttttaaaaat aaaattatta atatataata ttaatcatgt taataatatt
     2041 tttagtttat tatattctat aatagtggcc atgttgacta ttgtagtttt attaagaata
     2101 gttggtttat ataaatatat gcaaagatag attgttgttt actttactta ttaatatata
     2161 ggacaagata ctctaataaa tttgcctatt ttaatatctt ttacactaaa accaactaaa
     2221 gtttccttta ttatttcgtt ttgaataata caaagcataa tccgattgtt tgtataaaga
     2281 ctggaaagta tcagagttat tttctaattt agatatacct atagaacaag aattactctc
     2341 taggcataat tctaaaactt tctttgcttt attttcaaca ttctcttttg attgattact
     2401 atattcaaaa acagcaaatt catctccacc aagtcttcca ataatagcat catttacgaa
     2461 tactttagtc aaaatatttg aaaaatgtat tagtatttca tctcctcgaa catgaccaaa
     2521 gatatcattt acctgtttaa aattatctaa atcaaagatg taaaagtatc catgtgaaac
     2581 agttttaagc atttctttta tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat
     2641 aaggtctttt tctgatttta gtttaaattc atttgccatt gctagtgctt cttgtgcttt
     2701 ttcagttatt gttttagggt aatactcatc tttcatttgg tcaaagtttg gaatagaatg
     2761 gtgaacatgg ataagtttaa tcatatcatc tgtaactctg tataccatag taaatcgagt
     2821 atccatttct acatagatat ctttttctag gtaatctttt tctattaccc aaagagtacc
     2881 atatactata aaagcttcgt ctgtgacttt ggtacatgaa taccactcat ctataatctc
     2941 aaagtctatt tgctctgatt ctgtttggtc tttttcaaga cctataataa attcttctaa
     3001 cgtttcgtaa aattcatgtt ttcctgttcc aattaatgca aggttgtcat ctaataagga
     3061 aataataaat ttaaggtttt cttcttctgg atttaaaata taaatatgcc aaattttttt
     3121 tgataaatct cttaattgtt tttgtattgt attattttcc atagattacc cccttaagat
     3181 ttacagttta tattttaatg tattataagt tgaactctta ttacaatatt
//
LOCUS       DAELJO010000305         3218 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.307, whole genome shotgun sequence.
ACCESSION   DAELJO010000305 DAELJO010000000
VERSION     DAELJO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.307"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            13..1122
                     /locus_tag="KPB88_003746"
     CDS             13..1122
                     /locus_tag="KPB88_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0055978.1"
                     /translation="MIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKDYFIYLK
                     GSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINLFLGDYC
                     SRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPIKLPKPQ
                     PDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGLRLNELR
                     ELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLNDEEKDAL
                     FLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGFSIYDVQ
                     NLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            1226..1861
                     /gene="lexA"
                     /locus_tag="KPB88_003747"
     CDS             1226..1861
                     /gene="lexA"
                     /locus_tag="KPB88_003747"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG0055979.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2008..3165)
                     /locus_tag="KPB88_003748"
     CDS             complement(2008..3165)
                     /locus_tag="KPB88_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0055980.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         1177 a          465 c          460 g         1116 t
ORIGIN      
        1 aatgataaga aaattataaa agtacataat aaatcagata aacctgataa tattgttatc
       61 aaagaaaatg aactcataga aaaatgtcta cttttggaaa ataaacttcc tgtatttatg
      121 aaggattatt ttatatattt aaaaggttct gttgcagttt ctacacgact tgcttattta
      181 gaagatatta attttttttg ttcatatttg attgaaacca aagaattaac aaatgcagat
      241 tgtgtaaaag atattacaga agatgatttt aatacaataa aatcaagaga tataaattta
      301 ttcttaggtg attattgcag tagatattac aaaaatacag aaaaaaacac attgattttt
      361 gaaaataaca atagagcttt agcaagaaaa aaatcatcca tctctactct atttaaattc
      421 ttatacagaa actctcagat agataataat ataactgatg gattcaatcc aataaaactt
      481 cctaaacctc agccagatgc tattaagcgc cttgagatag atgaagttgc taagatgctt
      541 gaatcagtag aaacaggtga agggcttaca gagaaagaaa aggtatattg gagaaaaact
      601 aaattacgtg acaaagctat actagcatta tttgttacat acgggcttag attaaatgag
      661 cttagagaac tcaatatctc ctcttttaat ttttcaagag gtgaatttaa aatatataga
      721 aaacgtggga aagaagtttt aatgcctata aatcatactt gtgaacatgt aataaaagat
      781 tatttgcaaa acgaaagaac aagagatgaa ctattaaatg atgaagaaaa agatgcttta
      841 tttttgtctc ttcaaaataa gagaattaca gcaaaggcta taagaacact ggtaaaaaaa
      901 tatacatcta ttccacttga tactactaga gacaatggat atagtcccca taaattaaga
      961 gcaacggctg caacttctct aatccaaaca ggattttcta tatatgatgt tcaaaactta
     1021 cttgaccatg ataatgtcac aactactcag ctttatgcag ctcataagaa aaatgtaaaa
     1081 cgtgatatag tcaagaattt tgaatggatt gaggacgatt aaaaatcagg aacaaatgtt
     1141 tgcagatata aatggattat gttataatta aaaatataaa gtgtatggaa ataaaaacaa
     1201 aatatgtttg gaaatgagtg atgttatgta tttagatcta actgaaaagc aagtcttgat
     1261 attggaattt ataaagtctc aaatcatatt aaaaggttat ccacctgctg taagagaaat
     1321 atgtactgct gtagggttaa gatctacttc aactgtgcac tctcatctaa ataaacttga
     1381 aaaactggga tacataagaa aggatcctac taaaccaaga gctattgaag ttttagaacg
     1441 tagtaaagta aatgatgttt ctggagctaa tcaagaaata atagagcttc ctttagtagg
     1501 tcaaataaca gctggagaac ccattttagc tcaacaaaac atagaggaat acattccatt
     1561 ccctgccagt ttagtaaagg gcagtaacaa ttttgtatta agagtaaaag gcgaaagtat
     1621 gattaatgca ggaattttag atgaagatta cgttgtagta gacaaaaaaa atacagcctt
     1681 aaattctcag atagtagttg cacttataaa tggtgaatct gctacagtta agaggttttt
     1741 taaagaagga aatttaataa gacttcagcc agaaaatgat tttatggaac caattatgct
     1801 caatgactca gaagttgaaa ttgtaggtat tgtaactggt gtatttagag ttattaaata
     1861 gctattaaaa aatacttaaa aaaataagct attcgtatag ataaaatgtg ttatctattg
     1921 attatagctt atttttttat gcatttattt tattttttaa atgatttaaa gttcagaagc
     1981 tttatagata aattggttgt ttttttgtta ttttccataa catatatcac atgggtcatt
     2041 tttcttactt tctgcaattg tgcctgataa tattttttta cttcttgaaa gcccactaca
     2101 attttttgta gagtgataac tcttaccttt tggagtaaaa tacactattt cttctgaatt
     2161 ggatgtattt tgtttatttt taaaagaatc atttttactt gtattactgt ttgattttcc
     2221 agagtttcca tcttctctaa cgcctttatt ataacttcca tctttacaac ttgtaaatac
     2281 tccatcacct gttgattcaa atattatatc tgaacactca tcacttctat gtacttttac
     2341 tcccattttt tttaatttat tcattgtctc ctgatgtgga tgaccatatg aattaccctt
     2401 gcctactaaa agaaccgcat attcaggatt tactttatct aaaaactctt gagatgaact
     2461 actacgagaa ccatgatgag ctacttttaa aaggtctact ttctcaagct taggtagtat
     2521 ttctttttcg gtaccttctt cagcatcacc tgtaaataaa actttatcat tcccattagt
     2581 atatacaagg actagagatt gttcattagt agtatctccc ccatttgtat ttagaacttc
     2641 aaaataagaa tcttctaaat agaacttatt attttcaagc ggtacacttg gggataaacc
     2701 cttatttgct aaagcattta taaagtccct ataactcttt gtttctgctg aaccattcga
     2761 tacaaaaaca ttttctatat taaagttttt gacaacagaa tcaagtccac ctaaatggtc
     2821 tgcatgagaa tgggtagcta taagatattt aatatctttt acaccttgat tgtttaggta
     2881 atctaccatt aatccctcat catcattatc tcccccatct atcaatgtaa aagttttacc
     2941 atcttgaatc aatattgcat cactatttcc agtgtcaata aaatgaattt ttacattctt
     3001 atcacttgat atactactag acaaagaatc attattattt gattgattat ttccattatc
     3061 tgatgtagta caaccagtta aataaatcga tatagatact atcaataaaa gtatcaaagt
     3121 tttaatttta gaattttttt tattttttat acttatttct atcacaaact acgcctccat
     3181 attcataata ctctagtaat tataacatga tatgcgtt
//
LOCUS       DAELJO010000306         3184 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.308, whole genome shotgun sequence.
ACCESSION   DAELJO010000306 DAELJO010000000
VERSION     DAELJO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.308"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            174..890
                     /locus_tag="KPB88_003749"
     CDS             174..890
                     /locus_tag="KPB88_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0055981.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            865..>3184
                     /locus_tag="KPB88_003750"
     CDS             865..>3184
                     /locus_tag="KPB88_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0055982.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVGSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDISLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAEMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTIT"
BASE COUNT         1330 a          289 c          560 g         1005 t
ORIGIN      
        1 aaacaaaatg aaaaaatatt aaatttaaac tagaaggagg tttctaagat ggacattaaa
       61 tacaaataac cagaacaaaa gatataaata tatatataaa tggaaaaact tctatcatat
      121 tgtataatca tatatgtaaa gaaaatttat aaattaatat aaaggagatt agaatgaatt
      181 catataatat tttggtggta gaagatgaaa aggagatagc agatgctata gaaatatatc
      241 tattaaatca aggatataat gtttttaagg gatacaatgg attagaaggt cttaaggtta
      301 tagaaaacca agaaatacat ttagctatag ttgatataat gatgcctaaa atggatggta
      361 taacacttac aatgaagctt agagaaaact ataattttcc agtaataatg ctatctgcaa
      421 aatcagagga agttgacaaa ataatggggc ttaatatagg agcagacgat tatgtaacaa
      481 aaccttttaa gccattagaa ctattggcta gagttaattc acaacttaga aggtacacta
      541 agtatctaaa catggttgga aataaagagc aaaaggtaga aagtgacgat agtgtgtttg
      601 caataggcgg attagaatta aatgaaaata caaaagaagt tagtattgat ggaaagtata
      661 taaaaacaac tcctatagaa tttaaaatac taagtttact tatgagaaat gcaggaagag
      721 ttttttctgc tgatgaaata tatgaaagag tttggaatga gaatgctgta aatacagata
      781 cagttatggt acatgtgagg aatataaggg aaaagataga gattgaccca aaaaatccta
      841 aatatttaaa ggtggtgtgg ggtgttggat acaaaattga aaaaatttaa agataaatat
      901 atagttacta ttatagtttt tatacttatg ttatcagcat cattaggtat gattagtcaa
      961 tattctacta tacaagcagg tgctaaaggt ggagcaaaga atccatttga acaggaaagc
     1021 tttgtaggta gtatatataa aggaagttat gttttatatc ataatatgaa ggaagaacaa
     1081 gaaggtaaga tgatacaacc atcaaagcta tttatcaatg aagaaactat gaactatata
     1141 aaaaatgtaa gtaaagaaaa agatacttat gatggttcag aaacttacta tacagatgat
     1201 agtattaatg cagattttaa tgaaaacttt gaaaattggg agcatttcat taaagcgtca
     1261 ggaaagaatt taaagtacta tttagttgat aaagaaagca attttgaatt ttttaatgat
     1321 aataaagaat taggagtact tgatacaaca aagaatgatg cttcaaaaga aaattctgat
     1381 gaaaatcagt caaaaaatac agaaatgaat gatgaggaaa gacaaaagca gattacagat
     1441 ataaaagaaa actatagatt ctacgttgta atgaattttg ataaagatgg aaaagtaaat
     1501 attgtggatt cttatggagt aaatcaaaaa gttattaatc aaatgttgtt atctaaagca
     1561 agagaagatt taatggatat aagtttagga ggaaattcaa tttcatataa gttatctcca
     1621 ataaaaaata taacattagt atatgctgta cctaaagtaa taagtaaagg tgatagtgta
     1681 gagtatggtt atggaaatac ttattttgat gatggtataa tttcatatat aagcaatgta
     1741 gaaaataatt cttattataa gatttctgga atgattttaa aaattatatt agctatagtt
     1801 gttattgtag ctttagcatt cccatataga aaatctaaag agttgctagg atttgataca
     1861 ttatcaagaa taccgttaga gatactcttt attgctcttg ctattataca ggggcttgta
     1921 gatttatatc ctactgttat aataagtaat accttgagtg ggaattatgt agagtacctt
     1981 ataaacaatg acataagtgc tgaaatggct agtatattgg taagtttaat gaatttaact
     2041 tattggtttg taatattctc aataatattt gtatttataa tacttttaaa acatatttta
     2101 aatgttggaa taagagagta ttttacaaaa catactgtga caggaatgtt ttttgtatgg
     2161 ctaaaaaaag caggaaaaac agttattaat caaattagaa caataaatct aaaagaaaag
     2221 ccaaataaaa ctatagttac aattttggga ataaatttat tgattatagt tataatgtgt
     2281 agtatgtggt tctttggaat aatactcgca ttaatatatt cagtagtatt atttaagata
     2341 ctgtcagatt actctaaaaa gacaatatat gaatataatc aacttttaga tgttacaaag
     2401 aaaatttcag atggaaactt agatgtaaat atggaaaaag atttaggatt ttttaatcct
     2461 ataaaagacg aattaggaaa tattcaaact ggatttaaaa aagctgttga cgaagaggtt
     2521 aagagtcaaa agatgaaaac agagcttata tcaaatgtat cacatgactt aaaaacacct
     2581 ctaacatcta taatcactta tatagattta ttaaaagatg aaagtataac agatgaaaat
     2641 cgtaagttgt atattgatac attagacaga aagtcacaaa gattgcaaca tctaatagaa
     2701 gatttgtttg aagtaagcaa ggcaaatagt ggagatgttt atttgaatat agtaaatgtg
     2761 gatattgtat ctctaatgaa gcagacactt ttagaattag atgacaaact tgcagattca
     2821 tctctaatag taaagaataa cttttctaat gaaaaaataa tattaccatt agatagtcag
     2881 cgtacattta gagtatttga aaatcttata ataaatataa gcaaatatgc aatgccaaat
     2941 tcaagagtgt atattgatat tttagaaaca gataataaag taaatattat gcttaaaaat
     3001 atgtctgcat ctgagattga ttttagtgta gatgatataa tggagagatt tgtaagagga
     3061 gataaatcac gtaacacaga aggttctggt cttggtttag cgatagcaaa aagttttata
     3121 gaattacagg gtggtaagat gaatataagt gtggatggtg atttattcaa agttacaatt
     3181 actt
//
LOCUS       DAELJO010000307         3171 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.309, whole genome shotgun sequence.
ACCESSION   DAELJO010000307 DAELJO010000000
VERSION     DAELJO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.309"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..189)
                     /locus_tag="KPB88_003751"
     CDS             complement(<1..189)
                     /locus_tag="KPB88_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG0055983.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTL"
     gene            complement(221..358)
                     /locus_tag="KPB88_003752"
     CDS             complement(221..358)
                     /locus_tag="KPB88_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG0055984.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEIVGEDYI
                     A"
     gene            complement(359..658)
                     /locus_tag="KPB88_003753"
     CDS             complement(359..658)
                     /locus_tag="KPB88_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055985.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIEELPT
                     PKYTENKETKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(655..1886)
                     /locus_tag="KPB88_003754"
                     /pseudo
     CDS             complement(655..1886)
                     /locus_tag="KPB88_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1921..2103)
                     /locus_tag="KPB88_003755"
     CDS             complement(1921..2103)
                     /locus_tag="KPB88_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055986.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(2103..2399)
                     /locus_tag="KPB88_003756"
     CDS             complement(2103..2399)
                     /locus_tag="KPB88_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055987.1"
                     /translation="MDRANRIICDQTGKILLQTGEATGDVLPHNKITELHYIDIPYGS
                     IDYIKNRIVGINIETKQPILEEIPVYVSEAEKEKQELENQLLLLTNQEIGGGIL"
     gene            complement(2413..>3171)
                     /locus_tag="KPB88_003757"
     CDS             complement(2413..>3171)
                     /locus_tag="KPB88_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055988.1"
                     /translation="ALHTLKIVVTDSNNATAEKVLSISKNIMSLPENVNLNDISTKLV
                     EVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKASFDSSDASVQDLMNQLTQA
                     NNTITQLNSQFKVAGGTSVAQRSDGTKIAYEYKRSTTSKFGGWLKINGLAFKPNIFVA
                     DCEYYDSDYRVDYKFFTFACCGFTTSGKQDFVAVAIYSRSSGSDKYTGDGFIYNNNEG
                     DVWFNANGVQVPAYLPGGSESFTYAWRAVKFMNT"
BASE COUNT          975 a          569 c          332 g         1295 t
ORIGIN      
        1 aagtgttgta atcattgtac caattagcca tttgagcata cttgtaagtg agtttagatt
       61 ctcacacaat gcttttaact ctgctttaga ctctatattt gctattttta attcgtctat
      121 ttcttctcca tgtttattta ttcttgtttc atgtcttttt aaatctgctt cgaaaagttc
      181 ttcattcatg aaaacctcct aatttttgaa ttaaaaaaga ctatgctata tagtcctctc
      241 ctacaatttc tttatattca cttgctgtta tcttattctt ttctactgcc gttttaactt
      301 gctctttagt ccaattacca ttattataga aatctgttat tatcttgtac caattcattt
      361 atatcacccc atttgacatt aattgaaatg ttaaatctgc tattgtttgt tctgtagaat
      421 ttactttgtc ttctatgcta cttttaatat ctgtatatct atagaaaacc tctttagtat
      481 ctatatttat aaataacttt gtttctttat tttctgtgta ttttggtgtt ggtaattcct
      541 ctatcaaaat accttgtttt aagttttcct ccgataacaa atttggttca tagtgtatca
      601 taccaacata ttttatattt tgttcttctg tatccattaa atttcctaaa taaatcataa
      661 tgattctcct ttctcatccg aataaacctt ttttgacaat atatttctaa aaattccact
      721 actaataaag aaaactatat catttattac aaacatacca gcacttgggt atgtttcagt
      781 aagttttcgg tagctgtcga tacgtttaag agtatttaaa cttattttta caaaaggact
      841 actagaaagc gaatatatag catatatata tccattatat atatcaaaat tgtcatattt
      901 gctatcgtct gaatacacaa ttaaatttaa atttgcgtca tattttacta aagcactttt
      961 ttgttgctat caatttctat acctctttct gcatcagatg caataacaaa atcatttaaa
     1021 aacttaatgt ttttcatatg caaatatcct ccaattctaa aatctttagc aatggcaaaa
     1081 tcaaaattta ttttagataa atgacatgtt gtaatactac ttgaatttga gtgttctgtt
     1141 gtagcataaa tagcattctt attgcaaaca aacttacctc ctttaaaatt gtaaatacca
     1201 ttagaaacta aatttttagt taacattata tacatatcag atattctaat tttatgaagt
     1261 gtagaagaag tctcatctcc atatactcca taaataaatt ccccataagt acataactta
     1321 taataagcac cttctattga ctgcacttca attcctgttg tcttatttat tttatataat
     1381 ttagtattgt cagatataaa taaatactct tgtgtaacac agatacatga gaagttagca
     1441 ttagctaaag taatgtcaaa aactactgtt tcatcaatag cattaatttt aattagatga
     1501 gtttctttaa ttacataaaa atatggttct tcatattcaa aagttttgaa gttcccacta
     1561 catctttcaa tatattttat agctccattt gtaactaaat acggatagtt atttgaaagt
     1621 gttgtttctc ttaaatctaa tcgcccctct tttatatcaa ttttattctt tatttcttcc
     1681 catgtatcgc ttgtagtaac ctctgcacct ttggagttta atgctgttac tacattattt
     1741 ttagcattaa ctccactttg aaaaacctct tttaatgctc cttctacatt atcacttgta
     1801 aaattattct ctgtatcttc tatagttaca ttctttgctt ctaatacaag atttctaact
     1861 ttattaacta actctttaaa agtcatttag tcaccttctt tcaataaaaa aaagaaccta
     1921 ctacgctgtt ggttctattc cttctactac tccactattt tttataatat aatcctctac
     1981 tgcttttctg tattctgtgt tagttacatc atcaagttga aactctctat ttttcaaagg
     2041 gtttaaccct ccatttaata ttctttctgc taatattctt accacaacat tatttatatt
     2101 cattataaaa ttcctcctcc gatttcttga tttgtcaata aaagtaattg attttctaac
     2161 tcttgttttt ctttttctgc ttcacttaca tatactggta tttcttccaa aattggctgt
     2221 tttgtttcta tatttatacc tacaattcta tttttaatat aatctatact tccatatgga
     2281 atatcaatat aatgtaattc agttatttta ttgtgtggta atacatcccc tgttgcttct
     2341 cctgtttgca agagtatttt acctgtttgg tcacatataa ttctatttgc tctatccact
     2401 ttatcacctc tattaagtat tcataaattt aacagctctc caagcataag taaaactttc
     2461 actaccacct ggtaaataag caggaacttg aacgccatta gcattaaacc atacatcccc
     2521 ttcattattg ttataaataa aaccatcacc agtatattta tcgctaccac ttgagcgact
     2581 ataaattgct acagcaacaa aatcttgttt accacttgta gtaaaaccac aacaagcaaa
     2641 tgtaaaaaat ttatagtcaa ctctataatc gctgtcataa tattcgcagt cagctacaaa
     2701 aatattaggt ttaaaagcta aaccattaat tttgagccat ccaccaaatt tagaagttgt
     2761 agacctttta tactcataag caattttagt tccgtcgctt ctttgtgcaa cagaagtacc
     2821 acctgctact ttaaattgag aatttaactg tgttatggta ttattagctt gtgttaactg
     2881 gttcatcaaa tcctgcacac tagcgtccga actatcaaat gatgccttta ttttctctga
     2941 taattctact aaggtattat ttaaacttgc ttctatattt ttaagtgcta aagtgtttat
     3001 aatacttgtt ttcccaactt taaatcctgc attaacctca actaattttg ttgatatatc
     3061 atttaaattt acattttcgg gcagtgacat tatattctta cttatactta acactttttc
     3121 tgctgtagca ttattactgt ctgtaacaac tatcttaagt gtgtgtagtg c
//
LOCUS       DAELJO010000308         3150 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.310, whole genome shotgun sequence.
ACCESSION   DAELJO010000308 DAELJO010000000
VERSION     DAELJO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.310"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            269..1378
                     /locus_tag="KPB88_003758"
                     /pseudo
     CDS             269..1378
                     /locus_tag="KPB88_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1695..2363)
                     /locus_tag="KPB88_003759"
     CDS             complement(1695..2363)
                     /locus_tag="KPB88_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0055989.1"
                     /translation="MEIVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
BASE COUNT         1225 a          471 c          401 g         1053 t
ORIGIN      
        1 aaccagcaac aaacattgac actaacccta gtgataataa tttctttaat ttcacaatat
       61 ctcccccttt tatatatgaa atactatata tcttaataat tctacattaa attttctttt
      121 cctcctttaa cgatatttat cttcctgtta atttaagtgt atacaaatac gtgttataat
      181 gtaactgtaa agaattaaaa agataatgac ataattagag cagtccaact gttcttttat
      241 aaaactaact tataaaggga gtgtacatat gaatatcaaa tcagctttta taagaaaaag
      301 atgggaaaaa ttctatgtat atgtggaata agaaactggc aaaaagaaac aaaaaagtta
      361 tggaagctat aaaaagaaaa aggatgctga aaaacattta attgaaataa aatctactat
      421 aaacaataat aagtttgttg ctccaaaaga tatgacattt gtagaaagat gttataaata
      481 catagacatg aataaaaata attggtctcc atatactgtt acaaatacaa aatcttgggt
      541 taaaaattac atagaacctt tttttaagaa tctaaaactc acagaagtaa atcctagttc
      601 agtacaagct tttgtaaatt ataactttaa taattctacc tttactagtg ctaaagtaag
      661 gtatagcttt ctaagttctg taataaggga agcatataga ttaagagaaa tacaagaaaa
      721 ttcatgtgat tttataaaac ttccttctaa agaaaaatat tctacaaatg aaatatacaa
      781 tagagaagaa gtattattgc taatagaaaa gttacttggt aataatatag aaatacctat
      841 tcttttaatg ttacttctag gtttaagaat gggagaagct actgggctta ggtggtcaga
      901 tgttgactta gataacaatc tgataaatgt taatcaaatt cttgtctatt ctaatggaaa
      961 aatatctttt aaagagccaa aaacaccaaa atcaaaaaga gctttatcag ctccaaaaga
     1021 attaattgaa aaattgaaaa tagaaaaact aagacaaagc aaattaaaat tgcaaggtat
     1081 cctggaaaac aaaaataact tagtttgtct aaacaccaat ttaaatccgt ttctaccttc
     1141 ttcactaaga aaaacgtttc ataattttac aaaaaaaaat aatctaaaaa aaattagaat
     1201 acatgattta agacatacaa gtgctactct gctgcttttg ggaggtacta atatgaaagt
     1261 tgtttctgaa agattgggtc atacagatat aaaaataact atgaatagat attctcatgt
     1321 acttaaagaa atggacaaag aagcttccaa caatctaagc gaacttctat ttaaatagta
     1381 aaaactgact gtcagttaaa tgtcagttaa acctaaaaaa taggttttgt cagttgattg
     1441 tcagtcaatt attttataat agtttcactt aaaaacgtct atatctacac attttactat
     1501 ttatttatgc ttattaatat ctatctgtta ttattaattc taataacttc tatttaatta
     1561 tttagttcag tttacaagaa aatacacaca aaccataaaa tgagatgttg acaatataac
     1621 agacagtatg ctcctgtaaa aggtaacata ctatctgtta tattgtctgg attcccagat
     1681 tagctatttg gaacttaact aaaaaatcct aatataaggg caactattat aatggcaatc
     1741 ggaatacata ctgtgtaatc ctgttccata gagtcatcat ctgaatgtag ttttacaatt
     1801 cgcaatttag caatcagttt gaagcagaaa tagcctatta ttgttccaat tacattcgtg
     1861 agtaagtcgt ctatatcggt tgctctgtgt aacgtaaaaa gctgaatagt ttcgataaaa
     1921 agagataatc caaaaccaat caagaatgta tttctcaaac gttcaaaggt tttgctgata
     1981 agcggacata aaaaacctaa tgggacaaaa agtaaaatat tcaaaataaa actcaaacta
     2041 aatccatcac taaaaggaat aagatttatg tttggattaa agaacgtttc ccctaactgt
     2101 gacagtctta tatattcact gaatgtaggg ataccaacaa tatgcgatag tgttaaacat
     2161 aagtagtaat acattgccag tgataaaata gatgttttta cctttggaat atcactattt
     2221 ttcttgaata ttacaactaa gaaaatacca atcaagataa ttcccaacaa gaaaatagga
     2281 ttaattaaca ataaatcttt gatagcttca aacagactga gttgaatttc tacacctgat
     2341 tgattatttg ttacaatctc catttgtaca tacctcctca taacatttca attatttcta
     2401 aaaaaaatag aagataaatc ttaaaatcat cctatcaaaa tttttaaatt tgcctttatc
     2461 agtttatacc accagttaat tcctgtctta cttctgtatg gtattcttgg gattctttca
     2521 tcaaattaga caaaaaaatg gttaccatat caataaactg gttacgctcc atatctcccg
     2581 aagcaaccat ctcctgagaa taaatccgta tcaggaatct ccacatctac tgcacctatc
     2641 acaagttatt tgataacatc ctcaaacaat cccaacttgg atatatcgac aacacccatt
     2701 agaatgctac tgctgaccat ccttgttttc ctggtgataa ttttaatcaa ctggtaagtt
     2761 gaaacgccta ttttttttgc aagttccttt ttagaaacat ggtagctctt atatgaatct
     2821 gaatttcgtt gtctgggtgt ttcatataaa gcatagatac gcaccttttt tgcctatttt
     2881 cattatatag aaaaaaattt caaaatacaa taattatatt tttttcgttt aagcttagag
     2941 cagttccttg atgaacaaca ctttggaaac tgaatgattt acataccact taatacatta
     3001 actcgattta acattaatta taataatatt taaaacaaca actagggtat ataagaatga
     3061 actttttagt tctttaatca tttttttgta taaaatcaaa tctattttta taatttcaac
     3121 aattttataa tttcaacaat tttataattt
//
LOCUS       DAELJO010000309         3148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.311, whole genome shotgun sequence.
ACCESSION   DAELJO010000309 DAELJO010000000
VERSION     DAELJO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.311"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            220..1662
                     /locus_tag="KPB88_003760"
     CDS             220..1662
                     /locus_tag="KPB88_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0055990.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1704..2057
                     /locus_tag="KPB88_003761"
     CDS             1704..2057
                     /locus_tag="KPB88_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055991.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2248..3069
                     /locus_tag="KPB88_003762"
     CDS             2248..3069
                     /locus_tag="KPB88_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0055992.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1218 a          324 c          555 g         1051 t
ORIGIN      
        1 ttgttactaa atataggtac attagaacaa aaatctagta aattgtaact aaaatgtaaa
       61 tttttccttt aatctataca atattatgga aactttttgt aatttatgat ataataacaa
      121 atatataatt attaaattat aataaatgac tatagcgtag atgtaatact atgttatagt
      181 catttttgaa ttaaacttga tatatcaagg gggaagaata tgagaacaat tagaagagtt
      241 ttagcattag gtttaacatt agctatattt ctaataaatg tacctaatgt agatgcatta
      301 acatcagata caattaaagg taacaatata tatgagacgg ctggattaat agcagataaa
      361 aagagttatg atacagcaat tatggtaaat atggataatt ctatagcaga tggtctttca
      421 gcaagtggtc ttgcaggtgc tgttgatgct ccaattttgc ttgcacaaaa aaataagata
      481 ccaaatgaaa caaaacaaag attaaaaaat gttaaaaaga tatacataat aggtaaagaa
      541 ttatcaataa gtaagtcagt agaaactgaa ctaaaaaaca caggggcaca ggtaactaga
      601 ttgggtggag atgacagaat aaagactagt tatagtgttg ctaaagaggt aaatggtatc
      661 aaaaaagttg atgaagtgat attaactaat gcatataaag gagaagcaga tacaataagt
      721 gctgcacccg tatcagtaag agatatagct cctatagtac ttacagatgg aaagagtgtg
      781 cctttttcaa caagcaatgt aaaaacatat gcagtaggtg ggagtatatc aatgagcact
      841 agtttagtta ataaaactaa tgctaaaaga cttggtggtt cagatagata tgatacaaat
      901 aagaaagtta taaaagagtt ttatccagat gcatcagaat tttatttgag tgatggatat
      961 gatttagtaa atgcacttac aggttctaca attgctaagg aaaatccaat tgtattggtg
     1021 tcagaaagta gtgataagtc tatattagca ggagcagata agattactag attaggttca
     1081 ataagtgaca gtgtgtataa taagtgtgtt tctgctgcac aaaataatgg cgattcgtct
     1141 acaaaaggtg agtcacctat gaaaaatgag acaagcatat taggtcagcc aacagctagt
     1201 ttagaagcgt gcttaaaatg ggcaaaatct aaaaaagcta atgatttatt tatagagtta
     1261 ataccaatat tatatgatac tgctgttcag gaaggtgtta atcctgtttt ggcagtagct
     1321 caatctgcaa aagaaactgg tttttgtaat tttggtggag tattagatgc atcatttaag
     1381 aatccttgtg gacttaaaac ttctgtaggt ggttctgata ctgataagaa tgcccattca
     1441 agatttgata cttgggaaga aggaatatta gctcaaattc aacatttatg cttatatgca
     1501 ggacaagatg gttacccact ttcaaatcca gtagacccta gacatgaaaa atctttattt
     1561 ggtaaggcta aaacagttga aagtttatct aataattggg ctggaggtca atatggacaa
     1621 gatttagtaa gaatgatggg agaaattgag gcaacaaaat aattgaagat tatcaaatat
     1681 ccaataaata aggattgatt attatgaaaa agagtaatat ttttagtatc atatttccta
     1741 cagtcataat ggtgctaatg tttttaactt ttgctacaga tatatttaat attccagata
     1801 ttcaatctaa aagtattttt gttgtgggaa tggttttgat ttttccaatc tcttttttga
     1861 tacaagggat aatttgtgta ttaaataaga ctaattggat tttatctctt attgtgtctt
     1921 tagtaactta tatagtatta atgtatatat ttttaaatga ttctgcatat atatatgttt
     1981 tactttatac agctttttat atgattggac atataacagc taaactctat tacaaaatta
     2041 agacttttaa aaattaaatt gaagtatata aaagtaacat taagagaagt atccttaatg
     2101 attttctaaa atcagaaata ggtgcttctc ttttttgtaa aaataaaagt atgtaaataa
     2161 aatttaacca ttataatttt cttttaacaa atgtatacaa taaacttgtt gtataattaa
     2221 gatagataaa ataaagggag ggataatatg aatttaatag ataatcatat tcatactaat
     2281 ttttcaagtg atggaaagga ttccatggaa gatacaataa aaaaagctat aagtattggt
     2341 gttagatatt tgacttttac agaccattta gaacatgatg aagatagagg attttgcata
     2401 aattataatg actatgttcc agtatttaat aagtttaaag aaaaatataa aaaggacata
     2461 gagttattac taggtgttga agtgggttac agaaaacatc taaaaaatga aatagaagaa
     2521 ataataaatt ctaatccttt tgattttgta ttgtgctcaa ctcatacaat tgacaatgta
     2581 cctgttccat caaaggcgta ttttaaaggt cttagcaaag aagatgcgta ttataaatat
     2641 tttaatagca tacttgaaac taatcgtgaa tttggagatt acaatatata tggtcatttg
     2701 gattatatat ctaggtatgg aatatattca gataatagag ttatatacaa tgactttaaa
     2761 gatgttattg atgaggtctt aaagtcaata atcaataatg ggagtggtat agagctaaat
     2821 acttctggtt atagatatgg attaaatgct atacatccaa atgaagatat tctaaaaaga
     2881 tataaagaac ttggaggatt tatagttact gtaggttctg attctcatag ggtagaagat
     2941 atatgtaaag attttgatgt ggcctatgat atgcttaagt atcttgactt taaatatgta
     3001 tctttattta aagaaagaga aacttatttt gtaaatatag aaaaagttaa atctaacaat
     3061 atagcttaga gtgtgttttg aagtaaatta atggatagtt aaaaagttaa tagttataat
     3121 attgtatcct ctagaaaaat actagagg
//
LOCUS       DAELJO010000310         3122 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.312, whole genome shotgun sequence.
ACCESSION   DAELJO010000310 DAELJO010000000
VERSION     DAELJO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.312"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..562)
                     /locus_tag="KPB88_003763"
     CDS             complement(<1..562)
                     /locus_tag="KPB88_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0055993.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNY"
     gene            complement(562..1422)
                     /locus_tag="KPB88_003764"
     CDS             complement(562..1422)
                     /locus_tag="KPB88_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0055994.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            1557..2357
                     /locus_tag="KPB88_003765"
     CDS             1557..2357
                     /locus_tag="KPB88_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG0055995.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2382..2951
                     /gene="yfcE"
                     /locus_tag="KPB88_003766"
     CDS             2382..2951
                     /gene="yfcE"
                     /locus_tag="KPB88_003766"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG0055996.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1167 a          461 c          380 g         1114 t
ORIGIN      
        1 cataatttga aagatatact cctccaactt tactataatt aaaatgcttt acttgatata
       61 caatcatact aatcatcttc aaaagacttc catatacaaa gtaaattaaa atacttacta
      121 taaaaccaca aactttcatt tttccatatc caaacttaaa aattaaaggc agtaaaatcg
      181 tcaaaattgc taaaaatata tttacagaaa aaaatacatc atttaaaacc atatttctaa
      241 agaacactag tgatgtcaat ttacttagta aggacacaat tattgttaca gaaattatta
      301 atcctacacc taataaatat tttgagtaaa ctatatcttc tatactaact ggcatactcc
      361 ttataaaaat ccttgaatca ttttcataat cacactcaaa tgtatttata aatatcaaat
      421 aactaacaac aatacttact gtataatatg aaattgaatt aaggaaaaaa taaaacacaa
      481 aaaatagcaa tatgtacttt aaattacgga taccatctga actaatccct attattaaat
      541 cttttcttat taaattgaac atcatctcac ctcatttaca taataataca ttagttcttc
      601 tatagtagga tttttgacct tatgcttatc aatttctatc aataggcttt ttactttcaa
      661 gctatctcca actttaacaa gtgcctcatt ataatattta tactctttca caccaactat
      721 gctttcttta attgcttcta actctgcttt atttgcccta attatcttat attcttgttc
      781 tattaattct ttcttaacag taaaaatctg atttcctcta tttataaaaa caagataatc
      841 agcaatttta attaaattat cagtattatg agtagaaatt attacactac tacttccatt
      901 tttcacaaaa tcttgtaagt actgcattat ttcagttctt acaattgggt ctatcccagc
      961 agttggttca tccaaaatca ataacttagc attgtgacta agtgcgcttg ataacataag
     1021 cttttcagat tgacctttag ataaattttc aatatgcata ttttcttcta tatcaaactt
     1081 attaaggtat tcatcaaacc tttctctatc aaaattctta taaaataaag aaactatttt
     1141 tctaaattcc ttaaccttta aatgcggata aaattgtaaa gaatcatata caaaaccaat
     1201 attgttttta aagcttattg gagaattttc aatagtttca ttacaaagtt caatttcacc
     1261 ttcatctgca tcaataatcc ccattataat ttttattaaa gtcgtttttc ccgaaccatt
     1321 tggtcctata atcccaacaa tatccccttc ctttaactct aagtttatat tatttaactt
     1381 gaattttcct aatttttttg tgatattcct tatgctaaac acatcttcac ctcttatgca
     1441 tttttttata cccacctata catattttaa cacattcact acaaaggtca tttataaatt
     1501 tataaattaa gaattataaa actatgttat aatacaaagt atgaagaggt gaatatatga
     1561 aaagaattat aactataaat acaaaccaat taaataagtc ccttgagact aaagaactct
     1621 taactagaaa acttatcaat gctggctttg aagtgtattc agatatttat ccagatacag
     1681 agcttattat atcaattggt ggtgatggct cttttttaag aacagttaga gatttcgatt
     1741 ttcctgaaat tcccataatg ggaataaata caggtcatct ggggtttttt cctgatattt
     1801 taccagataa gattgatagc tttattgaag cttatacaaa aaaagattat attattcaag
     1861 aaatgtcact tcttaatgcg gaggtatata caactactag tggttctaat atgcttgctg
     1921 taaatgaggt tgttataaga ggagacaagt ctaggactat acacctaaat ttaagccttg
     1981 ataataaaca tatacaaaat tttagtggag atggtatgat tatttctact tccacaggtt
     2041 caacagccta taactattct gctggaggta gtattgttga tataaatctt gaacttatgc
     2101 agattacacc tttacatccg ataaacacca atgcatatag gtgctttaca tcaagtataa
     2161 tatgttcaaa tgaatctgtt ataaagattg caccagaata tagatttgaa gattctgtat
     2221 tgattgtcgt tgatggagtt gagcacagat ttagacaaat cgaaaatata aaagttagta
     2281 tatctgatgc aaaaataaaa ttacttagaa tgtcaaacta tgagttttgg catagagtat
     2341 ctgaaaaatt tttatagact gtacttagga ggatttttaa tatgaaaata ggaataatga
     2401 gtgatactca tggtagctta ctttactttg aaaaagcgtt aaatgtatta tctgattgtg
     2461 atgttttatt acatggtgga gatgtcttat atcatggacc tagaaatgat attccagaag
     2521 gatataatcc taaaaaattt attgagacat taaataaatt agaaaacatt gtgattgtaa
     2581 aagggaactg tgatgcagat gttgaccaga tggttataga acatcctatt cagagtcctt
     2641 atgtcatgag ccaatttggt gaaattagaa ttatattgaa tcatggttat atagaatcag
     2701 aagaagaaat tattgacaaa gcaaagaaaa tgggtggtga catattagtt ttaggacata
     2761 ctcatgtaaa aaaactatac atggatgata atttaatagt aataaatcca ggaagtactt
     2821 ctatcccaaa agatggaagc cattctgtcg caattataga tatcataaaa actgatgatg
     2881 aggatgagct tgaacttgat ataaacttaa ttgacatcaa tactggaaat ataataaata
     2941 tcaatgatta gtaaattttt aataaaaata aaataagctt taatttatta aattcataat
     3001 aaaaacaagt aaaccatttt atttctatct caattagaag tttaagtaca ataccttaaa
     3061 cttctaatta aatagaatct ggtttacttg ttttattata tgctatattg aaatataact
     3121 ta
//
LOCUS       DAELJO010000311         3077 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.313, whole genome shotgun sequence.
ACCESSION   DAELJO010000311 DAELJO010000000
VERSION     DAELJO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.313"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            171..1439
                     /gene="eam"
                     /locus_tag="KPB88_003767"
     CDS             171..1439
                     /gene="eam"
                     /locus_tag="KPB88_003767"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG0055997.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1532..1888)
                     /locus_tag="KPB88_003768"
     CDS             complement(1532..1888)
                     /locus_tag="KPB88_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0055998.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2039..3037)
                     /locus_tag="KPB88_003769"
     CDS             complement(2039..3037)
                     /locus_tag="KPB88_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0055999.1"
                     /translation="MISLLMSLLTPFIIAFVLAYIFNPLVKFLESKLNFKRIYSLLLT
                     YGVLIVILISFILFTVPSIVNSLADLVAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKE
                     YGDKIMSVMPKFSNLLIGSLGGIFSTTFSVGKFIVQFLLGFIICFYILLEKEKFLLFT
                     KKVVYISLGKKYGDFIIELCQSLNLNIGKYFTGKILDSFIVGVLSGIGLYFLKSEYAL
                     LFGTLMGVMNMVPYFGPVIGMAPVVIINLFSNPTIALTSLIYLIIIQQVEVTFIEPKI
                     VGGQLGLSPFFTILAVTVGGGFFGIPGMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1252 a          413 c          450 g          962 t
ORIGIN      
        1 aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa attaattcaa tttcatttgt
       61 tataaaatct atatggcaaa attaaatctg cctatcagaa tgatataaac acatcagaaa
      121 ggaggacgct atcaaataat acaagtagtt aactttgata gcaaaaaatt atgaatgaac
      181 aaactagaat atccttagag agagctgctg aattaaaatc aaaaattgat gattatattc
      241 aggctagaaa aacgattaac agaggtcttg aaaaagaaga agagataaat aaacgaaaac
      301 agaaaatatt aagtatctta aatggaactg aagaggattg gaataactac aaatggcaat
      361 tatcaaatag aataacagat gtagatactt tatcaaaaat tataactcta actaaaaaag
      421 aaaaagaata tataaaagag gttggtactc aatttagatg ggcaatatct ccatattatt
      481 tgagtcttat agacccagaa gatatatgtg acccaataaa attactgtct ataccaacac
      541 atatagagtt ggaagatgaa caagaagatt tggacccaat gggagaagag tatacaaacc
      601 cagcaggatg tataactaga agatacccgg atagattaat aataaatgta acaaatgagt
      661 gtgctatgta ttgtagacac tgtcagagaa gaagaaatat tggacaacaa gattctcata
      721 agtcaaaagc tattatccaa gaatctatag actatatcag agaaaatgaa gaaataagag
      781 atgtactagt aactggtgga gatgctctta cattaaaaga tgattattta gagtggattc
      841 ttagccaact taaagagata ccacatgttg attatgttag attaggtact aggactcttg
      901 ttacaatgcc acaaagaatt acagatgaat tttgcaatat gctaaaaaaa tatcaccctg
      961 tatatataaa tactcatttt aatcatccaa tggaaataac taaggaatct aaagaagctt
     1021 gtgaaaagtt agcaaatgca ggagttccat taggaaatca ggcagtatta ttaaatggaa
     1081 taaataatga taaatttgta atgagatgtt taaatcaaga attactgaaa ataagagtaa
     1141 aaccttatta tatattccaa agtaaacatg taaagggaac aaaacatttc aatacatcag
     1201 tagatgatgg tcttgaaatc atggagtatt taagaggata tacatcagga atggctatac
     1261 caacatatat agtaaatgct ccaaaaggag gaggaaagac tcctttgctt cctcaatacc
     1321 ttgtatcaaa aggaacagat tacgttatgc ttagaacatg ggaaggaaaa gttataaaaa
     1381 tggaagatga acctgctgta gatataaaga aacttataaa agaacaagca caggattaaa
     1441 aaaatattta aagtttataa aaagaaacaa ctttaaaata aaaagaaaat ggatgtcaaa
     1501 tttatatcaa tttgcatcca ttttttatta gttatttatt attttgttgt tgattaacta
     1561 attcaagata tccacttggg tggaagtaat atatctcaga agtattttca tttacaaagt
     1621 aaccaatatc accatctgca tctggtaaaa atacataacc attaagattt ttagctttta
     1681 atgcttcaaa atcactagca tttccttgat atatgtaatc aacttcattg ttgtatttta
     1741 ctaaaagttc tttagcttgt tcttgagtca atttagtatt ttggcttaca ggaacattct
     1801 tttgagaatc tgcataagaa gattcattta ctggtgcagt taataacaaa caagataaaa
     1861 ctaataaaga tataagtgct tttttcatac taaaaccctc tttcatgttt aattatataa
     1921 gattcattac ttaataatat tgtatgtttc ctataattaa atattaccat taaagagaat
     1981 aatattacag ttacacagct gtaacttttg attacaaaat cgtaattaaa ttgtattttt
     2041 attccatttg tattttatca tgtctacgat ttacaaactc acagaagtat atctttataa
     2101 cgcccatcac aggagcagat agaatcatac caggtatacc aaagaatcct ccaccaacag
     2161 taactgctag gattgtaaag aatgggctaa gacctagttg tccacctacg atttttggct
     2221 ctataaatgt aacttcaact tgttgaatta ttattaagta aattaaagaa gttaaagcaa
     2281 ttgttggatt ggagaataaa ttaattatta caactggtgc catacctatt actggaccaa
     2341 aatatggaac catattcatg acacccataa gtgtaccaaa taatagtgca tattcagatt
     2401 ttaaaaagta aagaccaatt ccagaaagta ctccaactat aaaagaatct aaaatcttac
     2461 ctgtaaagta tttaccaata tttaaattta agctttgaca tagttctatt ataaagtctc
     2521 catatttttt acctaaggaa atataaacta ctttttttgt aaacagtaag aatttttctt
     2581 tttctaataa tatatagaaa caaattataa aacctaaaag aaattgtact ataaattttc
     2641 caactgaaaa tgtagtgcta aatatcccac caagtgaacc aataagcaag ttactaaatt
     2701 taggcattac actcataatt ttgtcaccat actcttttaa tgtattaggg tcaactgatt
     2761 gtaaactctt acctaagtcg aataagaatt gctctgtttt atctatgtat gtaggaattt
     2821 gagcaactaa atcagctaaa ctatttacta tggatggaac tgtaaataat ataaaggaaa
     2881 ttagtataac tatgagtaca ccataagtaa gaagtaacga atatattctc ttaaaattta
     2941 atttactttc taagaacttt actagtgggt tgaatatata tgctaataca aatgctatta
     3001 taaatggtgt tagtagtgac attaataaac ttatcactcc aaaaaaatat ttgtaattat
     3061 caataacttt aatcaag
//
LOCUS       DAELJO010000312         3068 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.314, whole genome shotgun sequence.
ACCESSION   DAELJO010000312 DAELJO010000000
VERSION     DAELJO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.314"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            295..2430
                     /gene="rnr"
                     /locus_tag="KPB88_003770"
     CDS             295..2430
                     /gene="rnr"
                     /locus_tag="KPB88_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG0056000.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1350 a          284 c          534 g          900 t
ORIGIN      
        1 accaatccta tttgatggtg tataattagg tgataaagag taatttaagc aattatgata
       61 aagaataact taagtaatta taatgatatt tacaaataat ttataaaaca tcataatttg
      121 tttgaagtta gtatgacaaa gcttacatta aatcaaaaat ataaataagt aattttaact
      181 tataataata agaaaatata gaaaattatg tgataagtaa aaaatgtttt gtctgaagaa
      241 atataaaaga caatataaat aataatataa tgaaaagtaa aacaggaggg aaaaatgata
      301 ccaggactta aagaaagttt gctaggatta ataaatgaaa gtgcatataa tcctctaaaa
      361 aaagaagaat tagcagaaat atttgatata cattcatcag aaatgccaat gttttataat
      421 tttttaaatg aattagaaga agatggttat atctgcttta ctaaaaaagg aaaaatagta
      481 tctcccaatc aaatgggata ctttgttgga aaatttgtat cacacaaaaa gggttttgga
      541 tttgtagaat ctgatgttga atatacacag gatttattta tctcaagtga caatataaat
      601 ggtgctatgc ataatgatag agttatggca gaaatagtag tacctgcaac tgaagaaaag
      661 agagcagaag gaagaatagt aaaagtaata aagagagaaa taactagaat agtaggtacc
      721 ttccaatcaa gtaaaacttt tggatttgta acaccagaca acaagaaatt tactaaagat
      781 atatatatac ctaaaaaaca ttttagtgga gcagtagata atgataaggt agtatgtgaa
      841 attacagtat ggccacaaga ggatagaaaa ccagaaggca aaattataga gatactaggt
      901 caaaaaggtg aaagaggcgt agaaatagat tctataataa gagaacatgg tcttccagaa
      961 gaatttccaa agaaagtatt gcaagaagca gaagctgtag ctgttgagat tcctcaagaa
     1021 gagataaaaa gaagaagaga tttaagagat ttaaacatat ttactataga tggagatgac
     1081 gcaaaagact tagatgatgc catatcaata gaagtattac ctaatggaaa cttcaaacta
     1141 ggagttcaca tagctgatgt tacacattat gtaagagaga aaaataaact tgacaaggaa
     1201 gcgttaaaaa gagctacatc agtttattta gtagataaag taattccaat gttgccaaaa
     1261 actctttcaa atggagtatg tagcttaaat ccatttgaag ataagttaac tctttctata
     1321 tttatggaaa tagaccataa aggaaatgtg gttaaacatg aaatatgtga atctgtaata
     1381 aattcaaaag ctagaatgac ttatacagaa gtatctgata tattagaaaa ggatgatgaa
     1441 aaacttaaaa agacttttga acatgttgtt gaagatttta aaaatgctga aattttagct
     1501 aggattctta tgtcaagaag ggaaaaaaga ggagctatag acttcaattt cccagaggca
     1561 aagataattc taaatggaaa aggcgaagtt gtagatataa agccatatga aagaagaata
     1621 tctaataaga taatagaaga atttatgctt ataagtaatg agacaattgc cgaacatttt
     1681 tactggatgg gaataccatt tgtgtataga atacatgaga caccatcttt ggaaaaaatg
     1741 gaagaattga gtaagtttat atctacattt ggatatataa taaaaggtga taaagaggaa
     1801 gtacatccaa aagcattaca aggtataata gagaaaataa agggcaaaaa agaagaagga
     1861 gctatcagta ctataatgct tcgttcttta aaacaagcta agtactcacc agaatgtagt
     1921 ggacattttg gattagcagc taaattctac tgtcacttta cttcaccaat aagaagatat
     1981 cctgaccttc aaattcatag aataataaaa gaatctttaa ataataaaat aagtggaaaa
     2041 agacaagaac agcttactac tatagtggat tatgcatctg ttcaatcatc cgagaaagaa
     2101 agaaaggcag agttagctga aagagatgta catgatttct ataaggctct ttatatgaag
     2161 gataaagttg gtcaagaatt tgagggtgtt gtatctagcg taacttcatt tggaatattt
     2221 gttgagttag ataatactat agaaggattg ataagacttg caaatatgaa tgatgactac
     2281 tatatttatg atgagagttc ttatagtgtt ttaggagaaa gaactaagaa gtcatataag
     2341 ataggagacc ttgttaagat aaaggttgaa agtgttaatg tagattttaa agaaattgac
     2401 tttgagatag ttgaaaaaat agaggagtag ataaaacagc taaaataaga gataacaagt
     2461 tgtagtattt atgatatgta taaatatata gtgcacaata ctctataaaa taaatgataa
     2521 aaataggtaa ttgagaaata tctaatggaa atagttaact gaaagataaa taattaagaa
     2581 atatttaatg aaagtagcta attgaaaaat aagtaattga gaaatgttta atagaaagag
     2641 ctaactgaaa gataagtaat taagaaacat ttaatagaaa aagccaactg aaaaataaat
     2701 aattaagaaa tgtttaatag aagtggctaa ttgaaaaata agtaattaag aaatgcttaa
     2761 tagaagtggc taattgaaaa ataagtaatt gagaaacatt taatagaaga agctagctga
     2821 aagataagta attaagagat atttaatgta aacagataat ttaaaaatac ctaattaaga
     2881 atagatatta aaaaatataa tttcaatata aaaataagtg tatcatttaa aatttaagat
     2941 acacttattt tttatttaag ataacttttc tttttataga atttataaca ataggttaat
     3001 atatttataa ttaatgtaaa aattattaat atctattaaa tcaaattctt acacatataa
     3061 ttattgat
//
LOCUS       DAELJO010000313         3030 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.315, whole genome shotgun sequence.
ACCESSION   DAELJO010000313 DAELJO010000000
VERSION     DAELJO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.315"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..415
                     /locus_tag="KPB88_003771"
     CDS             <1..415
                     /locus_tag="KPB88_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0056001.1"
                     /translation="FEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQK
                     KGDLSEKEKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNI
                     SNKEFKRRYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            521..2758
                     /locus_tag="KPB88_003772"
     CDS             521..2758
                     /locus_tag="KPB88_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0056002.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYSLKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         1260 a          270 c          586 g          914 t
ORIGIN      
        1 atttgaagaa gataaaccac tagaaaaaag ttgttttaaa tgtggaaagt gtgtaaaata
       61 ttgtccggga aatgctatat tgggaaatta tgatatgaac cccaaaaaat gtttatcata
      121 tataactcaa aaaaaaggag atttgtcaga gaaagaaaaa gttgttttaa agaatggcaa
      181 aaaggtattt ggatgcgata tttgtcaaga agtatgtcca cataatgttg aaataccaac
      241 aactcatatt ttggaattta aagaaaatat aatagattat ttgaattatg atgagataca
      301 aaatatttca aataaagaat ttaaaaggag atatggagat agagctttta gctggagagg
      361 aagaaatata ataaagagaa atatggaaat aattttagat aagtatgatg actaataata
      421 agagtctgtt tcatatactt taatgtgtat ttactttaac acttgttgtg taaaagtcat
      481 attatatcat taaagttaaa tatggggagg tgggggaata ttgaaatata ataatacatc
      541 tgaaataaat ccactagatt atatattggg tcaagaaaga gctgtagaag ctatggaatt
      601 agggttgaaa ataaataatc cagcgtacaa catatatata gctggtgaac ctggaactgg
      661 taaaagtact tattccttaa aggttttaaa tgagtatgca tccaaaaaaa atacgcataa
      721 agattggtgt tatatatata attttgaaaa ccctagagag cctattatag ttgaattaga
      781 aaaggggttt ggaagagaac ttaagaaaga tatggagaaa ttaattgaaa gtcttttaga
      841 agattttaaa aatgcgtttg aaagtgaaaa ttttgaaata gaaaaaaata aacttctgga
      901 tgaatatgaa atagaaaaag atatgctttt gaagcaaatt aaaaaatatg gagaagaaaa
      961 aggatttaaa ttaaaacaga gtaaaatggg aatagttttt atccctttga aagaagagga
     1021 agatgaaagt gatgagtctg atgaagagtt ttacaaagct aaaagagaac tagaaaatat
     1081 ggcgatacag gtcgtatata aaattagaaa cttagaagaa atagctgaaa aagcagtgtt
     1141 agagcttgaa gaagagatag ctaaacttgt agtagaacct catatcaaga gattgtgtga
     1201 aaaatatgaa aattatgata agattcaaac ttatctggaa aatataaaga aagatataat
     1261 agaatatatg tatttattct atttagatga agaggagtta aaagataagt atgataaaga
     1321 gcattttatt aaatataaaa ttaacctatt tgtagacaat ggaagctcaa agaataaaga
     1381 atcagcacct gttgtagtag agataaaccc aagtccagca aatctatttg gaaaggcaga
     1441 gtatgattat gcaaatggaa atataaagac tgatttcaca aaactattat caggagcatt
     1501 tcatagggct aatggtggtt atttagtact ttatgcagac caattactaa aatatacaat
     1561 gtcttgggaa attcttaaaa agactttgca gactaaaaaa gtaatattgg aaacacaaac
     1621 agcaattaaa ccagaaaata tgccattgga tgttaaatta gtactgattg gaagccacta
     1681 tatatatgat attttatata gatatgatga agattttgaa aaatatttta aagtatttgt
     1741 agactttgat agtgaaatgg ataaaaatga agataatgaa gaaggaatag caagatttat
     1801 agcataccaa tgtgataaga ataatttaag gcattttact aaatcagcag ttgaagaggt
     1861 tattaaattt agcactagat tatctggtga tttagaaaaa ttatcaacta agtttaataa
     1921 aataatggaa gttgtaatag aaggaagtgc ttatgcagag tttagaaatt cggagtatac
     1981 agagcagtgt gatgttaaaa aagcaataag tgaaaagaga aaaagaatca atagagtgga
     2041 aacacatatg gatgaatcaa ttgaaaatgg atttactcta atagaaacag agggaagaaa
     2101 agtaggagtg ataaatggtt tgtcagtctt aagtacagga gaatactctt ttggtagagc
     2161 ttcaaggatt acagcgacca ctagcccagg aagtaaaggc atagtaaata tagaaagaga
     2221 agtaaatatg agtggctcta tacataacaa aggagtactt atattaggtg gctatctttc
     2281 tgagaatttt gcacaggatt tactattgtc actaaatgca tatgtttgtt ttgaacaaaa
     2341 ttatggtgga atagatggag atagtgcatc ttctgcagaa ttatatgtac tattatcatc
     2401 tcttagtgga gtatcaatta aacaaaatat tgctgttaca ggttctataa atcaaaaggg
     2461 tgatatacaa gtagttggtg gtataagtga aaagatagaa ggattttatt ctatatgtaa
     2521 aactaaaggg ttaaatggtg agcaaggtgt cataattcct agaaaaaatc aaagaaatct
     2581 cgttttatca gatgaagtta atgatgcagt gcgagatgga aaatttaaga tatatactgt
     2641 tgaaagagta gaagaggcta tagagatatt gacagatgtt aaatttgaag aaataaagaa
     2701 attggtaaaa gaaaaattaa tatctttcag caagatacaa actgtgtcaa aagaataagt
     2761 ttattataga atttaatggg tatttatgta taataaaaag caagaacttt aataggtttt
     2821 tgcttttttt gtaaatatat ataaattaaa ttcttatttt gtatgtttta tatattgatg
     2881 tttatttttt gttgattaaa aaatagttac gcacactcgt gactttagtc atgagttagt
     2941 agctaaaata gtcagcatat agggaaactt atatgtagag ataggataga acctatccaa
     3001 tactacttga attgctaaaa acccctaaag
//
LOCUS       DAELJO010000314         3015 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.316, whole genome shotgun sequence.
ACCESSION   DAELJO010000314 DAELJO010000000
VERSION     DAELJO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.316"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(71..679)
                     /locus_tag="KPB88_003773"
     CDS             complement(71..679)
                     /locus_tag="KPB88_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG0056003.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(788..>3015)
                     /locus_tag="KPB88_003774"
     CDS             complement(788..>3015)
                     /locus_tag="KPB88_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0056004.1"
                     /translation="YKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNH
                     TYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQR
                     KELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEK
                     LKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKD
                     YENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNI
                     DFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNK
                     LFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFREL
                     FGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFG
                     QKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQ
                     LIENLYKKGKIIDFQDRLKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFD
                     IIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLE
                     GGEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCL
                     TKSLIEIQNGKIYIDSKVGEGTSVKIIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIY
                     K"
BASE COUNT          961 a          495 c          237 g         1322 t
ORIGIN      
        1 tctataaaaa aataagatac atacctataa aataccttcc tatagataca tatcttacct
       61 atataaaaac ttattctata taaaaacgcc ataaaaaatc ttttgcttct tctgcataat
      121 ctatatttat acgtttacta gtttttatat ctaactcatc tttactaaat actttttttc
      181 ctttatcatc ataataaaaa tcacttatat ataattcttc acccattata ctcttactat
      241 ttagacttct atctattttt aaagctttgc aaagttttcc tggtccgtta gtaatatttt
      301 ttacttgata tttactcaac tcagtgtaag ctttgttgta tctattcata gatatctcat
      361 ctaaagaagt tataggctct acccctctta ttaatacaca ctctggcaca ttttctatat
      421 ttgcagataa attcaagcaa ttatacatac cataaattaa ataaacatat atatgtccac
      481 catctaaata taatggctta gttctatctg tctttttatt gccatataca tgtgctcctt
      541 tatcatttac acccatgtat gcttctgtct ctatgatttt ggtaacaatt actttatttt
      601 catatttcct tattagatat ttacctaata ttgatttggc taaatcaatt ccattttttc
      661 taaaaaaatc cttttccaaa atttcacctc ataaaattta tttaaaacgc atcaaataca
      721 actaaagtaa taaataaaat taactcttat taatgtgata tatataaaca ataaatttag
      781 acatttatta tttatatata tcaaaaaatt ctatctcaaa cttttcaata ttatcattgt
      841 aatttgaatt atcatatact tcttcaacaa cctctttaat tgggaatata atttttacac
      901 tagttccttc tcctacctta ctatctatgt aaatcttccc attttgaatt tcaatcaatg
      961 attttgtaag gcataaacct ataccacttc caaattcatt tgatatacca ctgttttcaa
     1021 tttgtttaaa tcgattaaat atatcattta atttttcttt gtctatgccc accccagtat
     1081 cttttataat tatctctact gtttcgtctt ttttatatat acctacaagg atttctcctc
     1141 cttctaaagt aaattttata gcatttgata atatatttag tattattctt tctatcgatt
     1201 ctgggtcaag ccctaccata acagtttctt catctgtatc aaaaattaaa tctattcctt
     1261 tagacttagc ataactacta atagatgtaa ctattctttc tgttatgtca ataatatcaa
     1321 aattaactgc tttaatctta tatatgtctg acttaacttg agaaatattt attatattat
     1381 ctattaatct caataatcta aacatatttt gtttagttat ttttatatgt tcttttagtc
     1441 tatcctgaaa atcaattatt tttccttttt tataaaggtt ttcaataagt tgaactgaac
     1501 tatatatatt atttaaaggt gtcttaagtt catgagatat attagcataa aactccatct
     1561 tcatttggtc atactgtctt tgttttatta atgctttttt aagtctttca gactctcttt
     1621 gcacacttaa atctttaata attctcactc tacttctttt ctgtccaaag tataatgttc
     1681 ttgatatcat ttttacttct ttaatgtgat tattttcatt tactatatgg atactcttct
     1741 ctctttctcc tatatttaaa ttgtttatgt atttttttac taatatattt ctatattttt
     1801 gtgttgtaac tcctaataca tcactagaaa ttccgaacaa ctctctaaag gctctatttg
     1861 tatatagtac tcttgatgta cttaaatcct caagaaaaat agcctctggt ataacatcca
     1921 gtaatttttc atatattatt ttttgttcat ttactgttct aagaatattt atctctttag
     1981 ttatatctct aaatgtaagc aatttaaaaa gtttattatc tgaccaatag tctccaactc
     2041 taacttcttc aacatgttct tcaccttctg agtcagacat cacaaaatta actatacagt
     2101 ttgctgtgtc attgtaactt actatttcag ctttcttttc ttgtgttaga tttaatcttt
     2161 taaactccct attgtcaaag aaatctatgt taaaagtttc aaaatattgt tctaatgtca
     2221 tatcgaccaa atcacttgaa acacatttaa aactcttttc atatttttta tttatactta
     2281 ttaccttatt cttctttata ccaccttcag cacttcttac aagtatagga gatggaatat
     2341 ttagtataat gttttcatag tctttattta tatttcttat ttcttcatct attttgattt
     2401 catttgtaat atcatttaaa cttattaagt aattgctttc atctttatta ttacttaaac
     2461 ttaacaaata aattttatca tttaactcta ccagtttatc aattatactt atatcgttat
     2521 caaatttctt caatttttca gcaacttttt catcttcctt tataaaagtt aatattttat
     2581 aataattatt catatccttt gaaaattcat ctttaaattt attatttgtg taacttatat
     2641 taaaatcata gtctgttact atccatacat ttggtattgt atccagagct tgtctaagta
     2701 attcttttct ctggtctatg tagtttttta atgttctttg tataatcata tccttttcta
     2761 ttgctaaatt tatctttcct aattgctctg actttttact taatttttta ttgattgaat
     2821 caagttcata atatggatac actactctat tattaagact gtatacatat acataataag
     2881 tatggttaca taatagtaaa tatattactc ctattaatct atctgaatct actatattaa
     2941 tcttagaaat tgctacaaaa catataatag ttgtaaaaaa aacaaaatat gttaaatctt
     3001 ttattaactt atagt
//
LOCUS       DAELJO010000315         2968 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.317, whole genome shotgun sequence.
ACCESSION   DAELJO010000315 DAELJO010000000
VERSION     DAELJO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.317"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            33..1142
                     /locus_tag="KPB88_003775"
     CDS             33..1142
                     /locus_tag="KPB88_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0056005.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1519..2703)
                     /locus_tag="KPB88_003776"
     CDS             complement(1519..2703)
                     /locus_tag="KPB88_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056006.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1118 a          446 c          406 g          998 t
ORIGIN      
        1 aaagaaaatt tttcatgaaa ggtaagataa caatggattt caaaagctta gggattagtg
       61 aaaatacaat aaatattcta aaaaaatcag gaataacaac acctacacct atacaaaaag
      121 aaagtattaa actaataaaa gaaggaaaag atgtaatagc agaagctcaa actggaacag
      181 gaaaaacact agctttttta cttccgatat ttgaaaatat atcattagat ataaatgata
      241 tacaagtact tatactttct cctacaagag aactagctat tcaaataaca gaagaagcta
      301 tgaaacttaa agaatctaaa gatgtaagta ttcttgcagc atatggagga aaagatattg
      361 gttctcaaat aaagaaacta aaaggaaata ttcatatgat tatagctaca ccaggtaggc
      421 ttttagacca tctaaataga aaaacaatag accttagtaa gttgaagact tttgttttag
      481 atgaagcaga ccaaatgttg cttatgggat ttaaaaatga agtggaagct attttaaaag
      541 agacttcaaa caaaaagcaa acactatgct tttctgcaac tataaattct caagtaaaaa
      601 aattagctta tagatacaca aaaaatcctg ttgtagtttc tattcaaaaa gaagaaatca
      661 ctcttaataa tataaaacaa gaagtagtag aaactacaga tagaaaaaaa ttagatgcct
      721 tatgtaaggt actagatgaa gataatcctt ttatggcaat aatattttgt aggactaaga
      781 gaagggttga caatcttgaa gaagctcttg ctataagagg atataattgt caaaaacttc
      841 atagtgatat tgctcaatca aaacgagaaa gaataatgaa atcatttaga aatttagata
      901 tacaatactt aatagctaca gatgtagctt ctcgtggtct tgatatatct ggagtaagtc
      961 atatatataa ttacgattta ccagaaacac cagaagacta catccaccgt attggtagaa
     1021 ctggtagagc tggtgaagaa ggatatacat gtgcttttat tgaccctaaa aatgaacgaa
     1081 tgttaagtga aatagagacg gctatagaat ctaaaattag tagaagaatt atagaattat
     1141 aatttataga ttgcttaaaa taccaatgcc acacaaacac tgtaatatat agaaagctac
     1201 agaaaacatg tatcaatcta tactttgctg ttatactatt ttatattaaa gaaaaaagag
     1261 gctatccaaa gtaggattag tctcttttat tgtcaaaata atgaaaatat atttgacttc
     1321 atataaaaaa gagtgcctta taaactaaaa gtccattttg agacaccctc tttttatctt
     1381 ttattcttag ttttatattg cttgtttata gttgtttaat attagatttt gcaaataatc
     1441 ttaataaaca tgcagatata ttatctatag tatccttatc tatagtattc tctttagcaa
     1501 tattttttgc tactacatct aatttacatt ttcatttaat tccgctggcc atttagcttc
     1561 tggattttcc tttgcaaact tcttattctt ataatgtcct agtgtaagga atggaattac
     1621 tatagtgaat agaccaagat aaccacaata tccatatcca tatttaacaa tattacttaa
     1681 tcctgtaagt gatataagca ttgacactac cattattgca caagctgtaa atacccttct
     1741 tttcattctt gaagaaagga aagaaagtgc tttatggtct tcaaatctat ttacaagacc
     1801 aaatatagta gttactcctg tagacattaa acaaaggaat aatgcaacat tataaattat
     1861 gtatatagta tgatttccag attgacctgc aacataaaga gtagggagtg ttgtttgccc
     1921 agcagcaata atttctctat accatcccat tagcatagtt acagcaagtc ctaatgcaat
     1981 tgcattcata acaaatgaaa ttatcattgc cttagtagct tcttttgagt tcttaagaag
     2041 tggtcttgaa catcctgcaa gtgtaggtat aacaaccgat tgaaaacctg cataaataaa
     2101 agtattaagt attggcttga tatagccagg tgtgaaactc gttctatttg ataatatttc
     2161 agttatttct ggtactttag cttttatacc aacaataaaa atagtaagag tacatactag
     2221 aatagctatt gtcattgctg tagctgcttt agatacaagc atagcaccaa atatagtaaa
     2281 gacaagaagt acaactccaa taataaatac agctacaaaa taatttactc ctatactttg
     2341 aaatactgct gctgcacctg ctataacagc acttacagct attaaaacca ttagatagta
     2401 atatatctcc catattattt caagttttgg atatggtctc caaagctcac aaaataaatc
     2461 cttataattc ctacaattgt tattattata cattatgata acttctctca tggtagcagt
     2521 aagtataatc attgctaaaa ttgccataag tggtgctgtc catccatact gcacatagta
     2581 ctgagtttct tgattccctg tagcaaatcc tcctcctgca tgtgaactaa aaagaacgct
     2641 tgcaacacca aaaatagcta acatagagcc cgttactttt gctttcttta cacttttttg
     2701 catgaaatca tcctccctaa aatgatattg aaagttttta ttttaacact ttattgataa
     2761 aacaataaca tttttgctca ttaaattcaa ctatattttt ccaatgactt tacattttca
     2821 acttatttcc acctttatat taaatatttt ttatatattt attttatttt ttaaatagta
     2881 ttcaaattca ctttgttttt taattttatg aaatatcttt tttaatgtta aatattatac
     2941 attttttctt tattattata taattttt
//
LOCUS       DAELJO010000316         2962 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.318, whole genome shotgun sequence.
ACCESSION   DAELJO010000316 DAELJO010000000
VERSION     DAELJO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.318"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(164..1180)
                     /locus_tag="KPB88_003777"
     CDS             complement(164..1180)
                     /locus_tag="KPB88_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0056007.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKESGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWN"
     gene            complement(1508..1690)
                     /locus_tag="KPB88_003778"
     CDS             complement(1508..1690)
                     /locus_tag="KPB88_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056008.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            2003..2422
                     /locus_tag="KPB88_003779"
     CDS             2003..2422
                     /locus_tag="KPB88_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG0056009.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            2553..>2962
                     /locus_tag="KPB88_003780"
     CDS             2553..>2962
                     /locus_tag="KPB88_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0056010.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIF"
BASE COUNT         1137 a          407 c          360 g         1058 t
ORIGIN      
        1 ttaaataaat ttagctaata ttttgttttt gttaaatata aaaagtgtct accataattt
       61 gtatagtaat atttgagatt ttaattatca ctattttttg taagaataca caatttatat
      121 ttaaatacct gacagaatat ttaaatataa atttgtttta aaactaattc catataactc
      181 taccatctat atccttaaca ttttgacaac cagtcaaaat catagtctca cataactcat
      241 tcctaacctt ctctataaaa aactccacac catcaagacc accgccaaaa gaagcagtaa
      301 caaaaggtct tcccatcaaa acagcatcag cacccaatcc aagcatctta actacatcaa
      361 caccagtcct aactccacca tcaacaagta ttgttatctt tccctttaca gacttagcta
      421 tatctggaag tacctcacaa gtaccaggag tataatcaag tactcttcca ccatgatttg
      481 aaacaataat ggcactagca ccagactcta cagccatcaa agcatcatct acagtcataa
      541 ttccttttat aataaaaggt aatctagtgg attcaaccaa ttctctaatc tcatcaattg
      601 ttttaggaga aaaaggattt tcttgaaatt gattattaat aagtccacaa gcatcaaggt
      661 ctacaccaac tgcaaaagca cctgcttctt cagacaatct aattttatct attatctttg
      721 aattattcca aggctttata aatacaattc catttcccct attatccttc aatacatcta
      781 aattatccag taaaaaagta tctacattag tatcgccaac catagcataa attccagaat
      841 tagaacaacc tctaacaaca ggctctatgt attctttttc ggaaacttta ccacccatgt
      901 tcaatatagt acctgataca ggtgcagcga atataggaga actcattttt ctaccaaata
      961 attctatgga tgtatctggc tttgaaacat tatgtatgac tctcatattt atctttattt
     1021 tttctagact ttttctattt tcaatgaaag acgaaccact ttctttacca cccataccag
     1081 gaacatctcc agcacaagct aatccattac aaattttaca taccttacat gttccattaa
     1141 agttctctct agctgatttt agtaagtcat tgtaattcat acaaaatctc tcctttttat
     1201 aatattaaaa ttgagtatta ctatattgaa aatatcttaa ttgtaaatat agggatgtta
     1261 ttatgatgag ctaaactaga taatacatat tagatatttt ttattgttgt agatagttaa
     1321 attatatcat attaaatagt caaattttga aaaaaactaa cttatttaag tatacatttt
     1381 gaaaaattaa aaaagctata tatagatttt taaagaaatc tatatatagc ttttaagttt
     1441 atgtcaatct tttttgttaa tgaataaata atatagaata caatatacaa aaaagtttct
     1501 tgaaatgcta agataatccc catttacaaa gcaacatcat tatactgtgt aaatttgaat
     1561 caacaatgtt ttcaccatat ttcatcataa aatcctctaa ttgtgtgtca ataaatgaat
     1621 aatatccact tatactatcg atgtatgcag tcattaagtg tatacattca tcacttttat
     1681 ctttatacat atatatctcc ccctatatac aatatataaa tttagatacc aaatcgtatc
     1741 atattaaata gttaagtgtg taacatctat gcagttatat atttatatca ttatattaat
     1801 aactaaagtg agttttgaca atagattttg ggaaactgga tgttcaaagt gcaaacttgg
     1861 attttaaaat cattaattaa gatagcgcat aggaatatat gtatacataa cttaattata
     1921 aagattataa acaattactt ttattaactt tatttaaatg gtagaataac ttggagatta
     1981 ataattataa ggtggatata atatgagttt atataagttt atatatgatg ataaggaata
     2041 tttattaaaa gaggagaact gtagtgcttt aataaatgat gaagaaaatc cagttcaagg
     2101 agtaagtatt tcaaaaataa ttgatatact taatgaagca gaagaagttg attttgatgt
     2161 agaatattat caagaagcat gtccattatg tttagaagga gtaaaagaaa agaaaaaatt
     2221 ctttcctttt ttagaatatc acttttatat tttcagtaaa gatggagaat atgtaataag
     2281 caatatatct gttgattata aaggactatc ttttaataaa ctatcaaggg caaataaagt
     2341 tgataatagt tatattgtaa gtgtgataat ttgcgaaaat tgtcaggatt atatagttca
     2401 gatagaaaat tgtatagttt agtgtaaagt atgtcttaat ttaagagaaa tttctaaatt
     2461 gagacatcaa cttttttcaa aaatagtatg gaaaattact aaatttatga gataatgtaa
     2521 gtcggaggtg agttacaata tatagtaaat atttggaaga gatagatgat acaattttag
     2581 attcaatgtt taaactaacc ttgaatgaaa gattattgac taataacgat tttattgaaa
     2641 ataaaattga tgtagtaaag aatttaattg attgctataa aactgaacaa aatagtttaa
     2701 tgttattaaa atcaaatttt ttgctagctt tattatatgg tattcaaggg tttagtgaaa
     2761 aaatgaaaga gtatattttg atttcctgta aatatattga taaatgctta cccaaagatt
     2821 ataaattttt agcacgattt tattcacaaa tagctatttt aagtttaaaa aatgaagatg
     2881 taaataaggc aaatttatat atggataaat ttaatcttat ttgtgatgaa aataattttc
     2941 taattgaaga aattattttt aa
//
LOCUS       DAELJO010000317         2905 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.319, whole genome shotgun sequence.
ACCESSION   DAELJO010000317 DAELJO010000000
VERSION     DAELJO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.319"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            20..814
                     /locus_tag="KPB88_003781"
     CDS             20..814
                     /locus_tag="KPB88_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0056011.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            815..2875
                     /locus_tag="KPB88_003782"
     CDS             815..2875
                     /locus_tag="KPB88_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0056012.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1100 a          302 c          486 g         1017 t
ORIGIN      
        1 gaaaggagtg gtttgataaa tgattgaggt agataaattg tgtaagtctt ttacaagggt
       61 tgtaaaagat gataagaata aaagttcaat aagaaagcta aagaaagtaa aaactaaaaa
      121 agaagaattt tttgctgtaa ataatgtttc atttaaagtt atggaaggtg aaattgttgg
      181 aatattaggt ccaaatggtg caggaaagac tacattactt agaatgttag gtggcatatt
      241 aacaccaaca tcaggaagta taaatatttc tggatatgat tattcaatag atagaaattc
      301 agcaaaaaaa gaaataggtt atctctctgg aaatacaaaa ctatatggaa ggctttctcc
      361 gagagaactt ctaacaactt ttgcaagtct atatgagatg tcaaaagaag atatagaaga
      421 atcaattgaa aatattgtaa aaataatgga tatgagtgaa tttatagata atagaattga
      481 aaatctatca actggtcaga ctcaaagaac atctatagct cgttgtttaa tacattctcc
      541 aaaagtatat atttttgatg agccaacact aggtctggat gttttaagta gtgcatctat
      601 tatagatttt atgaagaatg aaaaactaag gggaaaaact gttctttact caactcatta
      661 tatggaagaa gcagaaactt tatgtgacaa gatttttatg attcacaatg ggaaaataat
      721 tgcatcagga acaccacaga gtttaaaaga ggaatctgga gtaaataatt tgagagatgt
      781 attcataaaa cttgccagaa aggaggacat ttaaatgaga agtaagattg taaaatacat
      841 atttaaaaaa gaaatgttag acatattgag agataaaaag actttattta tgatgattgt
      901 tgtgccactt cttttatatc ctttacttat gttattaatg attcaagtaa tgaatatgtc
      961 tgcaagtagt atgaatagta aggacattaa tatagctttt aataataaac ctaataaggt
     1021 tttagtatcc atgattgaag atgataattt agaaaaagat actaaaaata cttcaaatga
     1081 taaaggcaaa ataagagtgt tagatgtaga taactataaa aagtcattag aagaaaataa
     1141 aattgatgct tatataaaga tggaagaaaa aaaatcatct ataaattatc aaatatatat
     1201 aaattcttct caagaaaatt cttctaatgc actaaaaaga attgaaactg ttttggataa
     1261 atataaaaga tttaccatag aaaaaacttt atctgaaaaa ggattagatg tacaagctac
     1321 tttagaacca ataacatata gcactgtaga tgttgcaaag acggaagaag tggcaggata
     1381 ctttttaggt cagatacttc catttatatt aataatagga gttttactag gttcaatata
     1441 tccagcaata gatgttatgg ctggagaaaa ggaaagaggt acattagaaa cattatttac
     1501 actcccaata tcaaacttag aactggttat gggaaaatat atggcagttt ctcttagtgc
     1561 aatagtaaca gcaattttaa acattttatc tatgggactg actatggcat ttattttagt
     1621 tagtggaggt attagtagcc aatttggctt tggaaacttt aattatggag atttagtctt
     1681 accaattata actactttaa tttgcatatg tcttttttca atggtagttt cagcaataag
     1741 tatgtgtgta tgttcattag caaaaagttt taaagatgct caaaactaca taacacctct
     1801 tatgttaatt attatgatac catcatatgt ttctatgata ccaaatatag tattagatag
     1861 agtaacagct gtaatacctg tagtaaatat atctttactt ataaaaagtg tattatcttt
     1921 taaaagcgat ttaaatttaa tagcattagt tttagcatca aatgtagcct ttgttatatt
     1981 ggcaatcata ctactgtcta agatgttcaa ttctgaggaa atactttttg gtaatagtaa
     2041 gagtttttct ttcttagaga aaagaagtaa tataaaaaaa gggtctatac caactgtaag
     2101 tgatggtgtt attttatatg cagtaggatt agttctattg atttatgttg gttctttagt
     2161 tcaaattaaa tttggtatgg caggtattat aatgacacaa ttcatgataa tttctttacc
     2221 gttattattt gcatactata ttaaagctga ttttaaagag gtatttagct tgaaagttcc
     2281 caaattaaag catgtgtttg ggagcattgt tctttggatt ggtggataca tacttattat
     2341 gattataaca cagctaattt tattcttatt tcctcaaaat atggaagttg ctgaaagttt
     2401 aaataaggct ctttttatca aagatagtgt tttactgaat ttactagcaa tagctttatt
     2461 accagcttta tgtgaagaaa tgttttttag gggatttata ttttcttcat ttagtaaatc
     2521 aaaagataaa aataagtcta tcaagctagc tataatatgc agtggggtac tatttggtat
     2581 tatgcatatg gattttatta gaataattcc aacatcaata ttaggtatta tatttgcata
     2641 tagtgtgtat aaatctggtt caatatttgt atcaatgtta ttgcattttt taaataatag
     2701 tgtagcagtt gtgttaaatc attatacaac tggaaatata accaacttat ataaatttgt
     2761 tgaagttgat ttttcgaact taaatgtact taaatttgcc ttaattcttc taataagtct
     2821 tatattaatt atgttaggat taagagtttt agatagaaag tctttaagaa attaagtata
     2881 aagttattat atagattaaa atatt
//
LOCUS       DAELJO010000318         2903 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.320, whole genome shotgun sequence.
ACCESSION   DAELJO010000318 DAELJO010000000
VERSION     DAELJO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.320"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            275..1546
                     /locus_tag="KPB88_003783"
     CDS             275..1546
                     /locus_tag="KPB88_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0056013.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(2185..>2903)
                     /locus_tag="KPB88_003784"
     CDS             complement(2185..>2903)
                     /locus_tag="KPB88_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBG0056014.1"
                     /translation="LKEAMKLKLIPHNKIVLFEDDLSIGNIFDVNDYELRENIFKIRN
                     VFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVSKFQDKNIKIALVD
                     EKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWDVLVKENSIFRVLV
                     GNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDWFILWRLEKMASLK
                     IVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1077 a          375 c          344 g         1107 t
ORIGIN      
        1 aaacgatatg gataaaaaag cttctgaaaa tataatcaaa gttctcttta aataacattc
       61 cattttatgt aagtaggcat tatatttttc ttaaatgtca cttaaatgtc agttatatat
      121 aataatagta aacaactgta tttcactaat atttgctatt atctaatagt atttatagtt
      181 tagatttgat ataatacatg tggtaaaatt ttgatttgta aaacaaaatt ttacctacat
      241 atattaaaac aaatttaaaa ctaaggagac ctatatgtta aaagtattaa aaggaaatat
      301 aatttttaca aaaacttcag aaactttcac agtatttgaa aatagttata ttatactgga
      361 aaaaggtaaa gtaaagggaa tatttaaaga gatacctaag gaatacaaaa acctaaaagt
      421 agtagattac actgataagc ttataatacc tggtatgaat gacctccatg cccatgcctc
      481 tcaatttaaa aatcttggaa tggctatgga taaagagctt ttgccatggc ttgaaactta
      541 tacatttcca gaagaatcaa attataaaga tatagaatat gcaacaaaaa tgtataaaaa
      601 atttgtaaaa gaactttgga aatctggaac tacaagaata gcagtgtttg caacagtaca
      661 taaagaagcc tctatgaaac ttatggatat attcaaagaa gttggtcttg gagcatacgt
      721 tggtaaagta aatatgaata tgaattgccc tgatacttta ttagaaaaca ctcaggattc
      781 tattactgat acagaagaaa ttataaataa gtatagtgat acagattcta ttgtaaaacc
      841 aataataaca cctagattta taccaagctg tacaagtgaa cttttaaaag gtttaggaga
      901 tttatgttta aaatataata ttcctattca atcacactta tctgaaaatt atgatgagat
      961 tgaatgggtt agaagtctag aacctgaatc taaattctat ggagatgcat ataatagata
     1021 taatttattt ggtcaaactc ctactttaat ggctcattgt gttcattgtt ctcaggatga
     1081 aatagattta atgagagaaa ataatgttat ggcagttcac tgtccagctt ctaactttaa
     1141 tgttggtagt ggtgctatgc ctatcagaaa attaatcgat aataatatta gattagcact
     1201 tggatcagat ataagtggtg gacatacatt gtcaatattt aaagcaatgg tatctgcaat
     1261 acaattatct aaattatatt gggttaactc tggtaagaag tataatttct tatctttatc
     1321 tgaggctttt tatattgcaa ccaaaagtgg tggaagtttc tttggaaaag ttggtagctt
     1381 tgaagaaggt tatgattttg atgcattgat tattgatgat tcaaatttaa atcatgacaa
     1441 ttattctata cttgaaagac ttgaaagatt tatatatgtt ggtgatgata gaaatatcat
     1501 tcatagatat gtatgtggaa aactaattga agaacctaat atataattat aataaaaatt
     1561 ttaattatat attatggtta atttatacaa ctataattaa taaaagagta tattttaaat
     1621 ctaaaagcta taggttgaaa catactcttt ttattttgtt tatttatagc tttagttatt
     1681 aatcatatat aaatctacaa aacgtattcc tagtaactta tattttttga tatctacaat
     1741 cataagacta tattattgga ttcatacctc aaaaatattg ccttctgaac attattatat
     1801 tcaaagttat gttaatttac aatgatatat ctaacatgta ttgattaaaa aaaattaaaa
     1861 cttgcttttc atctcctata tctaaagaag tggcatttag aataaactat tgtaatatta
     1921 aagttgaaat tttctagttt atttactcta tagcaccata ttttaatact attaagtaaa
     1981 aatgtagtta aaatatatac taatatctct gaaataagta tttctttttt atttagcaca
     2041 cttattcgta aggtattagt tattatattt tcaaattata gaataaatat tatacttatt
     2101 gctaattata atattaatac tgttaagata tttttcttca tagttatacc cattatttaa
     2161 tacaatttat tataaattca atttttaaac ttttcttatc tccttatcag aataaaaatc
     2221 ccctttactt ccttttattt ttactatttt taaacttgcc atcttctcta atctccaaag
     2281 tataaaccaa tcttttacac gcatttcatc ttcaaacata cagtttgcaa ccacttcaat
     2341 taaaggttgc catttgtcac tgatatttct taatatatat ttatcaaaat aagtttcttc
     2401 aacactttta atttcgttac ctactaatac tctaaaaata gaattttctt taactaacac
     2461 atcccattta tttttaaatt ctagtttttc attaaagcca atttctagtg tcttatctaa
     2521 aaagaatgga aaatcttctg gagacatttc actggtaaat gaatatctaa ttagagcatc
     2581 ttctgtcttt ttttcatcta ctaatgcaat ctttatattt ttatcttgaa acttactcac
     2641 tacataatat actccacaca aatcttttgg gttatttgca caccatatta ttatatttct
     2701 acattttgat aatttcctat cttctaacaa atctggcttg aataatagtt tcggattatt
     2761 ttcaaaaaca tttctaatct taaaaatatt ttctcttaat tcgtagtcat ttacatcaaa
     2821 aatattacct attgataaat catcttcaaa taatacaatt ttattatgtg gtattaactt
     2881 taacttcatt gcttctttta ata
//
LOCUS       DAELJO010000319         2880 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.321, whole genome shotgun sequence.
ACCESSION   DAELJO010000319 DAELJO010000000
VERSION     DAELJO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.321"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..997)
                     /locus_tag="KPB88_003785"
     CDS             complement(<1..997)
                     /locus_tag="KPB88_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0056015.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AGNIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDK"
     gene            complement(984..1751)
                     /locus_tag="KPB88_003786"
     CDS             complement(984..1751)
                     /locus_tag="KPB88_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0056016.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2039..2713
                     /locus_tag="KPB88_003787"
     CDS             2039..2713
                     /locus_tag="KPB88_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0056017.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT         1062 a          402 c          315 g         1101 t
ORIGIN      
        1 atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa ctgtatttat
       61 aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac cctagcatag
      121 ttacacttgt agctattaat attgctatta cagccaatac actactattg ctctttaatt
      181 taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa ctttttctct
      241 tcttaacagc atcaataatc atatacatag ttgatgaaaa aaataaaaaa gttccaggta
      301 ttattgtaac taatataaaa actgaaagca taatatttcc agcaataaaa taactaattg
      361 acattaaata agaaatagct attaataaaa caccaagtac acattttaac tttataaatt
      421 ttttaaactt atacttttct ggatatttat taaataactc tataattctc ttatttctga
      481 taactatagt atttcttatt ccaataacta taaatattat taaaaataca gctaatgtct
      541 gaactaaggc ctttatactt aagttcatat ttactactat catttcattc attaatttta
      601 caagcatcat agtcataagc tttgaaaata acaatcctaa aaatatacct attaatattg
      661 caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga tttttcctca
      721 ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa aatgagtttg
      781 aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta ttaatactca
      841 tcctggtttt ctctccaagt gaactcaacg cttcattata ctctatagat ttaaaactaa
      901 aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta aaaaaactat
      961 tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc tccaattaag
     1021 gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt tacaatttct
     1081 gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc acttatagca
     1141 tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa tagacaattc
     1201 aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc aagtactaat
     1261 gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc accagaaact
     1321 tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat ctcttttatt
     1381 ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat tatattttct
     1441 tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag attatcacgt
     1501 ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt tatataaata
     1561 tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga tttaccagca
     1621 ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt tattcctctt
     1681 agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc aacagataat
     1741 atttcttcca tagttaataa tcacctttca tcacaattag ttatttatca attctcacaa
     1801 atatattata tatattaaaa tggcttttaa acatttaatt atattaattt aaggttacat
     1861 tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa aatataattt
     1921 atcaagtaaa atatagttat attaatataa ttattttagg taataaggta gaaatatttt
     1981 aatttatcaa gtagaatata gttatgttaa tataactatt ttgaattagg aggtaagaat
     2041 gtttaaaata atggtagttg aagatgacgt ctcattgaaa aatatcatag caaagtgtct
     2101 tactaaatgg ggacatgatg ttcatcaaat agaaaatctt gaaaatataa tagaagagtt
     2161 caaaaactat aacccagaat tagttttatt ggacataaac cttccatttt atgatggttt
     2221 tcattggtgt aatgaaataa gaaaaatttc aaaagttcct ataattttta tttcttcaag
     2281 aaattctaat atggatgtaa taatgggtgt aaatttaggt gcagatgatt atatacaaaa
     2341 acctttttct gtagatgtct tagtagctaa agttaatgcc cttttaagaa gaacttataa
     2401 ttttgttgat aataactcaa atcagattat tcacaatggt gttactttag atttatcaac
     2461 tgcaacaata aactatgaag ataatactat tgagctaaca aaaaatgaaa tcaaaattct
     2521 ccatgagctt atgaaatata aggggcagat agtaagtagg aataaactca tgaaaaaatt
     2581 atgggataat gattggtttg tagatgataa tacattaact gtcaatgtaa atagaatacg
     2641 ctcaaaatta aatgaaattg gtttagaaga ttttatagaa acaaaaagag gattgggtta
     2701 tataatcagt taaaacaatt ctaagtaggt gaatttatga gctttaaaga ttttctcaag
     2761 gataaactag gttttttagt atacaatttt actttcttaa tttttacagt atcagttatt
     2821 atattttctc ctgtagatgt ttttctgact gatactattt tatatatatt agttgtaaat
//
LOCUS       DAELJO010000320         2878 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.322, whole genome shotgun sequence.
ACCESSION   DAELJO010000320 DAELJO010000000
VERSION     DAELJO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.322"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..367)
                     /locus_tag="KPB88_003788"
     CDS             complement(<1..367)
                     /locus_tag="KPB88_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0056018.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDD"
     gene            complement(381..1316)
                     /locus_tag="KPB88_003789"
     CDS             complement(381..1316)
                     /locus_tag="KPB88_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0056019.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(1445..2092)
                     /locus_tag="KPB88_003790"
     CDS             complement(1445..2092)
                     /locus_tag="KPB88_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0056020.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(2085..>2878)
                     /locus_tag="KPB88_003791"
     CDS             complement(2085..>2878)
                     /locus_tag="KPB88_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0056021.1"
                     /translation="ALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKK
                     VNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLEDKPLIYD
                     LVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISK
                     NTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNI
                     KKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
BASE COUNT          956 a          425 c          329 g         1168 t
ORIGIN      
        1 tatcatcacg agttccaagt ttttctatgt ttagagtatt tgatttttca tttaataaac
       61 tactctcata gcctttaggt atagtaagct ttaattctgc cttagaatca tcttttttaa
      121 ctgttaatat ataagataaa tcatttttca aatactttgt caaatcattt gataatcttg
      181 tgttatcttt gtcatatata acaatggggg tagattctgt ttttataggg ttttgaaaca
      241 tgctttctgt aaccatccca agaaaccaag ataaaatcag aggcaaagct aaaaaatata
      301 ttaaattaag tataaaggag ctttttaaac tctttagcat agtttttaca ctcatgtata
      361 atttcataaa atcacctcca ttagtccctt aaattttttc ctgttagatt taaaaatacg
      421 tcttctaggc taggttcttc ataagaaatt ttcattattt ttgaattgtt tttgtcaata
      481 acagagagta tatctgtaag ttgtaatttt ttatctatat ccaattctaa taatgaatta
      541 ttttctaaaa cagatataac acctttagta ttttttaaat caaagataag ttgggcattt
      601 atatttttta tttccataat caattttgta tttgtagata cgagtgattt taaataatca
      661 ttatctccaa aagcaatttc tttaccttca tccataataa aaattttaga acaaaggtgt
      721 tcaacttctt ccatataatg ggaagtatat attacagtag tttcatgttt tttagatata
      781 tcttttataa aattgaaaat atgatttctt gattgggggt ctacacctac agttggttca
      841 tccaatatta atattttagg atgatttaag agtgctattc caatatttaa tctcctttgc
      901 ataccaccag ataatttttt gacctttttc tttttgactt cagtaagacc tgtaacttct
      961 aatgcttcaa tgattctttc tttaagtagt tttcccttta aaccgtagaa agctccaaag
     1021 tactctaagt tatcaaaagg agtaagagtt tctataattg ctaattcttg tggaactaac
     1081 cctattatag actttgcttt tatataatct tttgataaat ctaaaccatt gatttgaata
     1141 ctaccactat ttggtaacat aagatttgta attatattta taagtgtaga tttacctgca
     1201 ccatttgggc caataagtcc aaatatctca ccttttttaa cagaaaagct gatgttatca
     1261 agtactaatt tatcattaaa tctttttgtt atattattta ctctgactat atccatagta
     1321 atccctccaa aataattttg tataagttaa aaagctataa tattataata aagaattgtg
     1381 aattataatt ctattactat aagtcactaa aaaaagtgac ttaagtcact ttatattttt
     1441 agatttattc tattacaatt ttatttttga aagcaaagat tgctagttga gttctgtcac
     1501 gaagttctag tttagctaaa atattagtta tgttattctt tattgtacct tcagataaga
     1561 atagttcttg acttatttct ttattattaa gtccatttgc aattagtcta attatagata
     1621 tttctttttc tgttaaattg tacatgtcat aaagatgctc ttgtttaacc gttttttcag
     1681 aggtcatgat tttttcagct acacttttat caagcacaat attgccaaaa aatgaagaac
     1741 gaatcccttc atgcaatact tcatcagagc tatcttttaa aaggtatcct gaagcaccaa
     1801 acttcatagc atcttttata tattcatcat cattaaaagt tgttaaaatt aataatgtta
     1861 tagaattatt atattccttt ataagccttg tacctagaac cccatcacat actttcattc
     1921 gtatatccat aagaactatg tctacatcat tcattttaca aaattctaaa gcttcatatc
     1981 cattagaagc atctcctaca atttctaaat cattataaga tgataatata attttaagac
     2041 cttttctaat taatttttcg tcatctacta taagaacttt aattttattc aaaaaaatca
     2101 ccccctgaaa atttaggaaa agaagctcta attgtgaaac cattattaga attatcaaat
     2161 tcaactttac catttaattc atatattctc tcattgatac tattaagtcc atttcctttt
     2221 tttatattgc cacatcctat accattatca ctaatagtaa ttataagatt tgaagtattg
     2281 aatgtaataa aaattctgat ttcagttgcc tttccatgcc tagatgagtt ggaaagggat
     2341 tcctgtatga gtctatataa agctatagat tgatttctgg ataagttcca agtatttttt
     2401 gaaatagtta atttaacatt aatatttgta agcttaataa agcttttaac taattctttg
     2461 attttaaata aactttggta attttcatat tcaacaggtt ttaactcaga tatagccctt
     2521 cttacatctt gaaaactttc agttacaaat tctctaagtt catgtactaa gtcataaatt
     2581 agaggtttgt cttctaagag tttttctata gcatttaatt gtattattgt tgtagaaaga
     2641 gaatgtccta cactatcatg tatttctctt gatattcgat ttctttcttt taaaattgct
     2701 aattcttcta tagaacttgc ataagtttcc aaatcagcat taactttttt taattcatct
     2761 ttagaaattc taagcttatc ataaagattt tgactgtata attttgataa attctcattt
     2821 tctatatata tagaaagtac agttataatt agcaatatag agcttgactc cagtgcca
//
LOCUS       DAELJO010000321         2864 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.323, whole genome shotgun sequence.
ACCESSION   DAELJO010000321 DAELJO010000000
VERSION     DAELJO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.323"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            255..1532
                     /gene="hflX"
                     /locus_tag="KPB88_003792"
     CDS             255..1532
                     /gene="hflX"
                     /locus_tag="KPB88_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0056022.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEELLNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSAKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINIEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1765..2262
                     /locus_tag="KPB88_003793"
     CDS             1765..2262
                     /locus_tag="KPB88_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0056023.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2311..2835
                     /locus_tag="KPB88_003794"
     CDS             2311..2835
                     /locus_tag="KPB88_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0056024.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1179 a          258 c          522 g          905 t
ORIGIN      
        1 agtagcttgt tttgtattac ttagatttgt aaggagtttt tttattgaaa aaactgcata
       61 atatatttgg gagggggtgt aagaaagtga ttttttatat aagattgaga aaataatgca
      121 cgattatggt tattataata gtaaataaga agtgtcattt aaggataaga taaaaagcaa
      181 aattcttgat gaatttaatt atagagccat aatctcgtgc tatagattaa ataaaaatag
      241 cagataagga gaatatggat atggcgaaaa aaggaataac agtaggatta aacataaata
      301 ataaatctga ggattttaat gaactaatga ttgaacttga aaacttatgt tcagcatgtg
      361 atattgatgt tgtagggagt ataactcaaa atgcaaagca ggttaataga gcattttaca
      421 ttgggacagg taaagttgag gaactattaa acttaataaa gaaagaaaac atagagatag
      481 ttatatttta caatgaactt tctacatcac aactaaaaaa tcttgaagaa aagcttaatt
      541 gtgaaattat agataggacg gcattgatac ttgatatatt tgcacaaagg gcaaaaacta
      601 gagaagctaa acttcaagta gaagtagcaa gtttaaaata tatgttacca agacttatag
      661 gttcaaatga aaatttagga cgtcaaagtg gtggagttgg tactaaaaat agaggttctg
      721 gtgagaaaaa attagaactt gaccgtagaa gaatagaaga aaagataaca tctttaaata
      781 gggagttgga tgatttaaaa ttccaaagag aaactcaaag aagtatgaga agaaaatcaa
      841 atcttccaaa tgtagctcta gtcgggtata caaatgctgg taaatcttca ataatgaata
      901 aactagtaga tatatttaaa aactctgaag aaaagaaagt gtttgagaaa aacatgttat
      961 ttgctacact tgaaacttct gtcagaaata tagttcttgc aaataacaaa gaatttttat
     1021 tatctgatac tgttggtttt gtaagtaatc taccacatga cttagtaaaa gcatttaggt
     1081 caacattaga agaagcatgt gaggcagacg tactgttaca tgttattgat atttcaaatc
     1141 caagctataa aagtcatatt aaagtgacag aagatacttt aaaacaaata ggtgcagatg
     1201 gtatacctat gatacatgtg tataataaaa ttgatttaat agatgtagac gtattggata
     1261 gaattttaga tagtattgat aaagaaggta tatttgtttc agctaaaaaa gatataaata
     1321 tagataaaat gataaaatgt atttgtgata gcatatttaa agattatgta agatgtaagt
     1381 tcttaattcc atatgataaa ggtcatgtag tttcttattt taacgaaaat acgagcatta
     1441 taaatataga atacagagaa gatggagcta ttttagatgt agaatgtagc aatatagaat
     1501 ataataagta taaaaagtat gctttagagt aaattttgaa aaacttatta tagcctaaaa
     1561 tatatttaat ttataattta ttataaattg attgtaaata tgattttatg aaacggtata
     1621 gccataatgc tatgccgttt tgccatttat aataaaaata aatttattga cacaatagta
     1681 actaattgct ataatataaa gtaattagtt actatttaaa attatcaaaa aattaaaatc
     1741 taagataaag agaagtgtat aataatggat gcagaaactt taatgaaaaa atatatggta
     1801 gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca aaataaactt
     1861 caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt acttttagct
     1921 attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt aatggggtgc
     1981 tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt tatacaaatt
     2041 gagcagaatg aaaaagactc aagagctatc tgtcttgtga caaaagatag agtggaagag
     2101 tattctaaaa aagtaagagg tatgcaggaa aaagtattac agcttttatt tgaagatttt
     2161 acagagaaag aagtggagca gttatttttt ggaattatga aattaagtat aggaattgaa
     2221 aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat caaatcaact
     2281 ttgtatgata tcagagaggg gagaatttat atgaaaagtg tagtagtgat ttataagagt
     2341 aagtatggtt caacaaaaaa atatgctaaa tggctttcag aattattgtc ttgtgatatt
     2401 tttgaaacta agaatattaa tatagaaaag gttatgaagt atgatacgat tattttagga
     2461 ggaggtcttt atgcttgtaa tatagcggga atttctttct taaaaaaaaa ttataaagaa
     2521 ttgcagaaaa aaaatttagt agtatttggc gtaggagctg ctccatatga tgatgaagta
     2581 ataaagaaat taagagaagt aaactttaag gatgaattaa acaaaattcc ttgcttttat
     2641 tgtcgtgggg catgggatga gacaaaaatg tcttttatag attatactct ttgtagtttt
     2701 tttaaaaaat ctatagctaa aaaagaacgt caaggtctta aaccagcaga agcaccttta
     2761 ttgtctggag aagcctttga ttggtgtaat aaagaaaatt taactccaat acttgattat
     2821 ataagagaac tttaagaggt atatatttat aattatttat tatc
//
LOCUS       DAELJO010000322         2849 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.324, whole genome shotgun sequence.
ACCESSION   DAELJO010000322 DAELJO010000000
VERSION     DAELJO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.324"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            35..886
                     /locus_tag="KPB88_003795"
     CDS             35..886
                     /locus_tag="KPB88_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG0056025.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGKLEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1019..2632)
                     /gene="ggt"
                     /locus_tag="KPB88_003796"
     CDS             complement(1019..2632)
                     /gene="ggt"
                     /locus_tag="KPB88_003796"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG0056026.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKYGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
BASE COUNT          913 a          478 c          390 g         1068 t
ORIGIN      
        1 aataaagata atataaacac aggaggtaca ttctttgaaa aacattattt cagcaaaaga
       61 acttatatca aaactagaaa aaaatgataa tctagttgta atagattgta gatttgatct
      121 tatcaataga acttatggta ttgattctta taaaaaagga cacatcaaag gcagttttat
      181 cttagatata gacaaggatt tatcatctcc tacaaaagaa catggtggta aaaatccttt
      241 acaagaccct ttaatactaa aagaaaaatt agaaaaaatg ggtgttgata atgacactac
      301 gattgtaact tatgatgatg gagatttaaa tggagcttgc agattatttt tccaattaaa
      361 acatttgggg cttaaaaatg tctatgtact ggatggggga ataacatctt tcgtaaagga
      421 aggtggaaaa ttggaagaga aagtgaatat accaagttgt acaggtaagg aaattagacc
      481 aaatataaac aatgatttag ttgttccaat ggaatatgtt aagtctaaat tatacaaaaa
      541 agatactgta ataatagatt gccgtgctaa tgagagatac caaggtttag ttgaacctgc
      601 ttactcaaaa gctggtcata taccaagtgc aaaaaattat ttttgtaagg atttaataaa
      661 aagtgacttt gaaaatggct cacttaaaga tatagaattt ttaaaaaaat tctttaaaga
      721 tttaaataat tatgatgaag taattttatc ttgtggttca gggatatctg cttgtgttaa
      781 cagtcttgct cttagagaac tggatatacc tcacaagatt tatattggta gctttagtga
      841 ctggattagt tatgatgaca atgaaatcaa aactggtcaa gaataaggct cataatctat
      901 ataaaatcag catatgataa cttaaatttt gtaaaggcgt cagtcattgt agactttcgc
      961 ctttacttat tatttcatat actctttaaa tttattatat atattttagt tgataatatt
     1021 aataaactgc aatatgacca tcacatcttg attccgtccc acaaatataa ctttcctgtt
     1081 ctgttttaaa tattatttga cctcgtccca tgtcaacagt atcatgaatt acttttattt
     1141 catgaccctt ttcaattaat gaatctatta tatgctttgg catctctggt tcaacttcta
     1201 tttcttttcc ttttatccac tgccatcttg gagcatctag cgcctcttgt ggatttaatc
     1261 caaaatcaat catatttgtt aacacttgaa tatgtccttg tggttgcata aaagctccca
     1321 ttacaccaaa tggacctatt gctttatctt ctttacctaa aaatccaggt attatagtat
     1381 gataaggttt tttaaatggt tttactacat tgtggtgttt agggtctaaa ttaaaattat
     1441 ttcctctact atgtaaagca atcccagttc ttggtatcac aattcctgaa ccaaaattta
     1501 tgtaattgct ctgtatatat gaaaccatat ttccatactt atctgcagta caaaggtata
     1561 ctgtaccacc acaatatggc tctcctgcag ttggatacaa agcctcttta ttgtcaatta
     1621 ataaacttct ttttttggca tactcttggc ttagtaattc ctgaatcttt accttcatat
     1681 gctctatatc agttacatat gttttagaat ctgcaaaagc taattttaaa ctctctataa
     1741 ttttatgtat gtcttctaca ttttctatat ttccttctaa ttccaattct tttaatatgt
     1801 taagtgccat taaaactgtt attccatgac catttggtgg gatttcaaat attttatatc
     1861 ccttatattc tgtacttata ggttctaccc atgttggata aaaacactct aagtcactct
     1921 ttcttatagc cccattaaat tttttagaat attcatctat tttatcagca agtcttcctc
     1981 tataaaagct ttcagcctga gtatttgcta tctcttctaa tgtacttgcc tgttcttcac
     2041 atataaatat atctccagcc tctggagctt taccatcttt agaaaatgta tcaaaccatg
     2101 gtttgaactc ttctccaata agttcttttt catagagttc atatgatttt ttccatacct
     2161 tagctacatt aggagatact acgtatccct ctcttgcata gtttatcgct ggagataagc
     2221 attccattaa gctaagttta ccatattttt tatttaattc tgaccaagct gctggtattc
     2281 caggtacagt tactgctcca aacccatatt ttggcatttc tttcatatta ttataatttt
     2341 ttaattccat attttctgga gcaaagccac tagaatttaa accatacaat cgtttttctt
     2401 cctctatcca aacaagggca tatgcatctc ctccaatccc attgcttgta ggttctacta
     2461 ctgctaatgt ggcggctgtt gcaactgctg catcaacagc atttccaccc ttttttagta
     2521 tttcaagtcc agcttgagat gctagtggtg ttgatgttgc aactgctcca tttttagcat
     2581 acacgacatt tcttattgat gagtatttat gttggtaggc attaaacttc ataaatttca
     2641 gctcctcgtc attttttatt tgatattctg cttagattta ttatataaca tcaattaatg
     2701 taactgatac aaaatgatat aaaatataga ctttttccta actttaattt tatctttaaa
     2761 taaatatctt aaactcaatt ctagtatttt ctatctgcac ttaaataagt tattttattc
     2821 aaaacatata tctaattctt ttaacttaa
//
LOCUS       DAELJO010000323         2848 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.325, whole genome shotgun sequence.
ACCESSION   DAELJO010000323 DAELJO010000000
VERSION     DAELJO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.325"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(660..1289)
                     /locus_tag="KPB88_003797"
     CDS             complement(660..1289)
                     /locus_tag="KPB88_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG0056027.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1368..2330)
                     /locus_tag="KPB88_003798"
     CDS             complement(1368..2330)
                     /locus_tag="KPB88_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0056028.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
BASE COUNT          960 a          441 c          298 g         1149 t
ORIGIN      
        1 tctatcatct caatcatatc atttactaac tattattcta atgagcatat aacattgaaa
       61 tttataatta tacaacctaa atgcctccca atgtaccctc aaaaacatca tttcctgtaa
      121 ctgaagtata attggtatat tttatcactg aaataaaaaa acactagtca ataaagacta
      181 gtattttcaa gtattttatc attctttttt taattattgg agacgaatca tcagaataat
      241 gcccataatt tatctatatg caaattctaa atttaaaata aagtttcaga tgaatttact
      301 attctatgct atgatacaac agaaaaaact gaaatctatg agtggtagct tcatataatc
      361 ctagttccta cagtaatcaa aactgaggtt tattctatat gtaactaatg ataaatttta
      421 ttataggagt tattagctct atttgtgtta ctataagtag taattatata tatgataatt
      481 ttttaacact tttctgttgg ctatatagaa agtggtttag aatttaaatt taaataaaat
      541 ttaaattcac caaaaagaaa tctatattag aaaccactac tctattccac cagattgtaa
      601 ttttttgttc tttatatcac aatgctatac catattaaat tgaaaaataa attttaaatt
      661 tatttgttac aattcattaa ttcaaaaaaa tattttgagt catgagtttt gtaaagattt
      721 agccatgaat ttaatgcttc tgattcaaaa acatctaaaa gtttcaatac ttgaaacgta
      781 aattccaacg gagcgattcc tgaagccgta actaaatttt catcactaac tacagattcc
      841 gttttataat atttttctcc agagtaattt ggtattacca ttttaagaaa ctctaagtca
      901 ttacttgtat gatttctaac atctaatagc ccttttttag ctaggcctaa tgtagctcca
      961 catatggctg caactataga atcttgtatt aaagcttctt taactttttc taatattatc
     1021 tcatgattca tggataacca agtatttccc cctggcaaaa ttaataaatc cttactttcc
     1081 atattaaact catcaactgt aatttcaggt aaaactttta acccacccat tgttataata
     1141 ggatttttat caattcctac agtaattact tttagtggct ttaatccttt tttaaaatat
     1201 cttcctgtat ttaattcagc agttaaatat gccacttccc aatctgacat agtatcaaat
     1261 acatacatat atacttttct atttttcatc ttttttactc cttatgtaat taatttatat
     1321 atctaattat aaaataactt aactgacata ttcagtcagt taagctatta aatttgataa
     1381 taatcaatta attctagtaa atcttcaatt attttcttcc taaggcttag tggttcaatt
     1441 acttgtattg tctttctata aggtaaaagt atatttggta tgtacttgca tattacttcc
     1501 tcttcgagca taaatattat tttattaggt gttctttctt tcaagtagtg ccctaaaaac
     1561 caatgttggc ttaaactatc taaagaatct atactccctt ctacaactaa ttgaattata
     1621 ttttgttcat cttttatttc aggtataatt ccttttataa aaaattcact tgctgaaaat
     1681 tctgtcggtc tgttaaatgt atttgatgtt tctatgattt cactaatcct atccactctg
     1741 aaactacgta cctcatttct catattacaa aatgcaacta cgtaccaatt attattccag
     1801 tgaacaattc catatggatt tataattcta tattttgatt ttgctttacg acctgcatga
     1861 tagttgattt ctacagatag ctcctttaat atactattct ccaatttttt aagtactgat
     1921 tctatagaca ctttcccaat tggttttatt acttcaaggg ctcttaagtg gtttgttaaa
     1981 atatcttctt ggttttgatt tgaatacatt tttaacttta atatcgattt atctaatgct
     2041 tcgtctaaga agtatccttc ttttttagca aatagagctg catgaagtag agatttttgc
     2101 tcttctatat caaaaaaaag aggtgcttta gtaaaattat ttaaaaggct atacccacca
     2161 ttatgacctg tatctgaaat tataggtact ccactcgcag aaagtacatc aatatatcgg
     2221 tacactgttc ttatatttat ttctaatttt tcagctatct gttttgctgt aatttttttg
     2281 tcagagttta acatccacag tatagacatc atattgtcaa ttttagccat gtagtttcac
     2341 ctctttctat ttgttatata aagttattat ataacttttt gattgatttt ttacaaaaaa
     2401 acattagtaa actgctaata atttatacta agatttattt caaatattta tagatagtct
     2461 ttttctatag ttaagttatc cattaaatta catagtagta tatccatttt agttgatata
     2521 ttttcagcta cctcccttga atattattac aataagtcta aatcttcttt cttccataaa
     2581 tatattatga ggaaagacta tcatctttaa tggtttaata gtataaaaga tattaattta
     2641 aaaaaatcta tttttatatc agatgggtat tgttttttat aaaactgaaa aaatcaccta
     2701 atagaatcta tgatttttta acataacctc cataaattag taccaattat cacttcaaat
     2761 tcaaattttt atctgaagaa tatacaatca tatttccata ctcaacataa tattgagtgt
     2821 ttggcttgta tttacaaatt attattct
//
LOCUS       DAELJO010000324         2838 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.326, whole genome shotgun sequence.
ACCESSION   DAELJO010000324 DAELJO010000000
VERSION     DAELJO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.326"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            61..2418
                     /locus_tag="KPB88_003799"
     CDS             61..2418
                     /locus_tag="KPB88_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG0056029.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2504..2821)
                     /locus_tag="KPB88_003800"
     CDS             complement(2504..2821)
                     /locus_tag="KPB88_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG0056030.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1055 a          326 c          473 g          984 t
ORIGIN      
        1 attttataat atatttgtag atttgagtat aaaaaagtgc taagttgaaa ggagtaaaat
       61 atggataagg ttaatttaga aaggtatagt tgtgatataa atgttggttt aaataaaaat
      121 caacttcaaa gtagaataga tgataattta attaatgcat ttgataatgg aaaaaccaaa
      181 acatataaac aaatttttaa agacaatata tttacacttt ttaatctcat aaatattgtt
      241 ttagtctgtt tattagtttc agttggttct tttaaaaata ccttatttat atttattgct
      301 gtaattaata ctattatagg agtaatacaa gagataagag ctaaaagatt attggacaat
      361 ctatctgtta tagtttcaaa taaggtatct gtcataagag aaaaagagaa aaaagagatt
      421 gatgtacaag aattagtatt agatgatatt atgattttaa aaacaggtaa tcaaatttgt
      481 gcagattcaa aagttttaaa tggaaaatta gaggtaaatg agtccttggt tacaggtgaa
      541 tctgatatta ttataaaaaa taaaggcgat tttttatatt ctggaagttt tgtagtttct
      601 ggagaagctt ttgtaagagt tgaaaatata ggaaaagata actatgcaaa taagatagct
      661 aatgatgcaa agatttctaa aaaacataat tctcaactta gaaattcatt aaatgcaata
      721 ttaaaaattg taggtataat aataattcct ctaggaatta tattgtttgc aaaacaacat
      781 tttggaaatg gagattctat agctacatct gtagtaggta cagtggcagc tgtaaatggt
      841 atgataccag agggattaac attacttacg agcattgctc tagctgtagg aactataaaa
      901 cttgctagtc ataaaacttt agtgcaagaa ctgtactgtg tagagactct tgctagagtt
      961 gatacactat gtcttgataa gacaggtaca attacagaag gtaaaatgca agttgatgat
     1021 atagtaatgt tggaagaagt ggatattaat gaaataatgg ggaatatatg taattctctc
     1081 aaagatgata atgcaacact aaatgcaata agagataagt ataatgtttt agatacgtat
     1141 aaagctaaga atttaatacc attttcttca gaaagaaaat acagtggggt tacctttgaa
     1201 aataaaggta catatttatt aggtgcattt gagtttatat ttaaggaaaa aaataaaaaa
     1261 cttaggatag aagttgaaag atattctaaa aaaggaaaca gagtaatagt tttagctcat
     1321 agtgattctt atatggaaga taataatatt cctgaagata taaagccact agcttttata
     1381 ttgatattgg ataaaattag agatgaagct aaggaaactt tagaattttt ttacaatgaa
     1441 ggtgtagata taaaaattat atctggtgac aatcctataa cagtaagtga ggtagctaga
     1501 aaagcaggtc ttaaaacagc tagtaatttt attgatgcaa caacacttaa aaatgatagt
     1561 gatatataca aagcagttga taaatacagt gtttttggaa gggtaagccc acaacagaaa
     1621 aaacaaatga ttttagcatt aaaaaaacaa aatcatacag tagctatgac tggagatggt
     1681 gtaaatgatg tgctagcact taaagaagcg gattgtagca ttgccatggc atctgggagt
     1741 gatgttacta agaatgtgtc taatttagtc ttattagatt caaactttgc atcaatgcca
     1801 aaggttgtta tggagggaag aaaagtaatt aataacattc aaagagcatc ttctttgttt
     1861 ttagtaaaga caattttttc attcttcttg tcgttattaa cattattctt cttcagtaga
     1921 cagtatccat ttgttcctat acaattatca ctcattgggg ctgtaacagt gggtatacca
     1981 tcattcttct tagctcttga agcaaacaaa agtagaattt caggaaattt tttactcaat
     2041 atattaaaaa atgctttacc aggagcacta tgtgtgacag taaatgtaat tattgtttac
     2101 aatatagttg aatatttagg ttatagttca agtgtatttt ccacaatgtg tgctatatta
     2161 actggtgtat gtggtctttt aatattaaga caacaatgtt tgccatttaa taaagaaaga
     2221 ttatttttaa tagtaagtat gactattgca tttgttattg ggattctatt tttaggtgga
     2281 ttattctcat ttgtagcatt gagtagagaa ttaattctca taactatggc attagcaata
     2341 ttaatgccaa ttatgataaa agttatgtta tttgtcttag atgagatatt agagacaatt
     2401 gttccagatt taaattaatt ttaaaatgat ataaataaga gcagttttag ttgaactgct
     2461 ctttttagat gtgtttaatt ttatgtaaga atatgtattt ttattaatca gctatatatg
     2521 agcaacagta gtcaacaact tcattaacct ttaagtcaaa acttgaatca ctaggaacat
     2581 taaatttttg cccttcttta taagttacaa actcattgct tccaggcagt tttacatcca
     2641 tacttccagc aagcatctcc attatttctt cttctctagt agaaaaagtg tattctcctg
     2701 gcaacattaa tcctaaagtt tttctttctc cattaggcag tattattact ctgctactta
     2761 ccttaccatc aaaataaacg tttgccttct ttactgcagt aacattctta aattcactca
     2821 ttagataaaa cctccaaa
//
LOCUS       DAELJO010000325         2822 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.327, whole genome shotgun sequence.
ACCESSION   DAELJO010000325 DAELJO010000000
VERSION     DAELJO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.327"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..423)
                     /locus_tag="KPB88_003801"
     CDS             complement(<1..423)
                     /locus_tag="KPB88_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0056031.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNN"
     gene            611..1516
                     /locus_tag="KPB88_003802"
     CDS             611..1516
                     /locus_tag="KPB88_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG0056032.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1525..2709
                     /locus_tag="KPB88_003803"
     CDS             1525..2709
                     /locus_tag="KPB88_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056033.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKLFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKIYIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
BASE COUNT         1124 a          251 c          454 g          993 t
ORIGIN      
        1 attgttatct atgtttaaaa catcattttt atagagatgc tgaatagata agtttttatc
       61 cgtacaagaa tttattagat tttgataagc ctcactatca gttgattgtt caggcatata
      121 tatggagttt acattaaatt tttttataat attatcaaga cttcctatgt ggtctgaatc
      181 tggatgagtt gctattataa tatcaatagt ttttattctt ttttgtctaa gataactaat
      241 aattatattt tcgctatctt catcacctcc atcaattaat atattcttat ttgtgggtgt
      301 ttgaactaaa atactatcac cttgtccaac atcaatcata tgtatagata gtagagattt
      361 tttgtcacag ccgattaaaa gtgatattat aataagtatt gataaaaaaa ttttatttct
      421 caataaaaaa tcactccatt cattacatgt ttatataaat ttatgatggg taatataaga
      481 tatatatgac ttattatatt ttatatttta aacaatataa atgaaattaa taccataata
      541 aaattggagt tttttatata tacaaaataa aatttttaaa attaagcaat aaaaaaggta
      601 ggaggatttt atgtttttca aagatattga actaaattca aaaaaagagt tagacccata
      661 ttttgattta gtagattatg aagcttgtga gtactgtttt agtactttat atatgtggca
      721 acatgtttat aagacaggat attatatagg tgaagatttt gccgttttag ttggggaata
      781 cgaaggagat agcttctcaa tattaccact tgctaaaaaa gataaacttc cagaagttgt
      841 agattttgtc cttgaatatt tttctaaaaa taataaaaaa atatacttaa gaggtattac
      901 tactgaagta gtagagtttt taaaagaaaa atatccaggt agatttgagt atatagaaga
      961 aagagattta tttgactata tttatgatgc agaaagttta agaactctag ctggtaaaaa
     1021 gaatcaaaag aagagaaacc atataaacta tttcttaaaa gagtatgcag gtagatatga
     1081 agctaaatta ttagataaag aaaactttga tgagtgttta gtactaatga aggaatggga
     1141 aagtaataag gaagaaaata atgaatttga tgaaagtatg gatgatgaat taataggtat
     1201 caaaaagata tttaaccatt atgacatatt aaaagataag gtaaaagtgt ttggtgttta
     1261 tgtagatgga aaattagaag cttttagtat aggagagctt ttaaatccaa atatggcatt
     1321 gattcatata gaaaaagcta atcccgatat aagaggtctg tatccattta taaatcaaca
     1381 atttttggta agtgaattta aggacgttga gtttgtaaat agagaagaag atttaggtat
     1441 tgaaggatta agaaaagcta aactttctta tcatccttgc agatttgttg aaaagtacag
     1501 tgttagggag gcttaataga ctatatggag attaggtatg ctaaagaaga agagatagaa
     1561 agtataaaag agatttggag ttattgtttt aacgatacgg agagttttat gaagtactat
     1621 tttaatgata aatataaatc tgaaaatact gtagtagctt tagatgaagg aaaaattatt
     1681 tcttctcttc agctaaatca gtataagtta ttactaaata gcaaggtata taatacatca
     1741 tatgtagtag gagtttctac acttccagag ggaagaggag ctggatatat gaacaaagta
     1801 atgaaattta ctttaaacga attgtataaa aaagggcaat tagtttctat attgatgcct
     1861 attgactata gactttatag aagatttgga tatgagcatt gttacgacca gattgaatat
     1921 actattaaca cagatgattt aaaaaatttt aagtcaagtg gaaaaatgat aaagtctaat
     1981 ttatcacaaa ttgatgattt gatacaaata gatagaactt ttttaaatga ggttaatggt
     2041 aatgtgttaa aagatgaaca ttattatgaa aacttattta aagagattca aagtgaagat
     2101 ggatttttat acattcatga aggaaatgaa aaagatggat atatagttta ttttcttcag
     2161 gaagataaac tgtttgtcag agagttattt tataaaaata tagatgccct taaatcaatg
     2221 cttaaattta tatataatca caatactcaa tgtaagatag ttactatatc tactcctaca
     2281 atagataaga taagatttat attagacaac cctaaagata gtgatataaa aataaaacct
     2341 tttatgatgg gaagagttat aaatgtaaag aagtttattg aagatataga tattgagaag
     2401 gatattaata gctcatttaa tttattaata gaagacaagt ttatagatga aaataatggt
     2461 ctatttaaaa tttatataca gaataagaag gtatctgtag agcagttaga taaaaaaggt
     2521 gcagaaaagc ctcaagaaga ttttgatatt aaattagata taaatacctt aacacagtta
     2581 tcattttctt atatagatgt aaatgaagct attttcttaa atgatataaa agatgtttca
     2641 gaagaaacac tagaaacact taattgtatt ttttctaaaa aaaataacta tataaatgaa
     2701 tatatatagg tagagtacgt taaaagattt taaaaatctt ttgacgtatt tttttgttta
     2761 gataacaatt tatcttgttt aaaaattttc tatttgaaaa taatctaaat aatatacttt
     2821 ta
//
LOCUS       DAELJO010000326         2816 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.328, whole genome shotgun sequence.
ACCESSION   DAELJO010000326 DAELJO010000000
VERSION     DAELJO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.328"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(62..232)
                     /locus_tag="KPB88_003804"
     CDS             complement(62..232)
                     /locus_tag="KPB88_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056034.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(621..1115)
                     /locus_tag="KPB88_003805"
     CDS             complement(621..1115)
                     /locus_tag="KPB88_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0056035.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            1919..2296
                     /locus_tag="KPB88_003806"
     CDS             1919..2296
                     /locus_tag="KPB88_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056036.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDSKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            2401..2776
                     /locus_tag="KPB88_003807"
                     /pseudo
     CDS             2401..2776
                     /locus_tag="KPB88_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1170 a          308 c          336 g         1002 t
ORIGIN      
        1 taataaaaca aatagaagat ttaaaatatc aatagcatat cagaaaataa caaatgatat
       61 gctagaattt ataaaatata tctaatgata aaaatggaaa atcaattata aaaaaggcat
      121 atcccgattc attttttgtt atatagtcaa ataagaatac tatagatgaa agatacgatt
      181 ttgcatatgt attaagtaag tattctgatg tttttggttg atacacatgc aataaagaag
      241 ttgtgtttgc agataaatta gcaaaactat agtataagtt gcaacaataa agaaaatgaa
      301 aattatcata ctaaagcata aggcatgtta caattttatt agataggaca ttaacataca
      361 ttaaagtact ttttattata aagagtactt atttttttga aattcattaa tgtataaact
      421 atcaagaata ttactcaaca taatttaaaa acaacttgaa taggtatttt taaattatgt
      481 gatataataa aaaatagaag tgtatgccaa tacacttctg taattataac taagcattct
      541 attcttgagg agtgcttttt tcatttctcc aaacaagatt gtttatttca tcagcaatta
      601 ttaaataaaa tttattgtat ttaagtattc gatttatcat ttaaaaattt tattacaata
      661 tctattttgt tttgctcgtt cattatgtct attgaacttc cataatcact ttttaattta
      721 tctagaccac ttttgcttat ttctccttct ggaagtatac ttaattctag atatttttca
      781 tgaccaggag atgacattaa tagatatgaa ccattataag gcatacctaa attttctatt
      841 ttatctgcta atgggtcatc tttaaagcca ccatttatta ttatttttcc atctttcttt
      901 acccatgata tcattcctcc atcagtatat tttgattcat ctgcatagta gctaagagac
      961 gcacttttta ttgcacttat atcagcttca agtttagcta tctttgcttt ttctatattt
     1021 ttaaataatg ctggaagtgc tactattgcc aatattccta ttatagcaat tactactaat
     1081 aattccacta gagtaaatcc ttttttattt ttcatttgtt tcccctccat ataaaattaa
     1141 taatatataa atttttattt tgttgatttc tactttctat attttaccat atatagacaa
     1201 aaaaatcact ttctatgaca aatatactat tactttacaa tttatggaaa tgatttaaaa
     1261 tgacatttct tgttagaaaa gatataagaa atggattaaa ataaagaaaa ctacagtcat
     1321 atcaatgtca ataatattat ttaaaagcag atgaaaataa aaagaggact cagaaaataa
     1381 tgtctattta attatactta cttaccaata ccataacttg atataaacaa gattatacta
     1441 cattttttta tgctctagta taaaatcatc taactgtgta tctataaaat caaaataatt
     1501 atatgaacta tcagtataat aagtcaaaat atgtatacat gaatacatat tttaaaagat
     1561 atatattatt acatacctaa aaaactttca taaaaacaaa aataaaatat gcttttagta
     1621 acttgttaac ataacttata atttatttta taagcattag cctatatagc tttgtgctac
     1681 taaatttctt tgattctagc gatttagtgc tattctacaa ctaaaataat acaatagagc
     1741 tatttattgt ttggtagcat tgtttgcaaa ttaaacaaat ttatatatat gaatattatt
     1801 ttacgtttaa aaaaacagac taaatatgta aaatatttgg taaaacttac ctctagtttt
     1861 tgtcttaaaa agctcctatt attaatttaa gttagaagaa ttaaggaagt gacataaatt
     1921 gaaagaagaa atattaatag aaaaattatt aagagcagaa ttaatagtaa tggagtattt
     1981 atggaaaaaa gacactctaa tgcctaaaaa agaaattgta aaagaaataa aacagattta
     2041 tggatggcat aaaagtacaa taaaaatact actaaaaagg ctagttgata aaaggtattt
     2101 ggctagatat attataaatt ttcaatctta ttataaaata ataatagata gcaaagaata
     2161 ttatactttt aagaaaaagg tactaaaatc tagtaaaagc ataaaaataa tgcattcact
     2221 taccactata cataaaaatg taagtaaaga gaagttagac ctattagatg agtattatag
     2281 aaacttaaaa gaatgatata gtaaaaatcg aatgtaatta tttaccttaa ttcctttgtt
     2341 tgtaaaactt tattgatata gaatttgaca tatacataaa ttggtaaagg gatgtatact
     2401 atgaaaatta gtaaattacc agaagcggaa ttaaaagtta tgagatatat ttgggaatca
     2461 gaaaaggtat taatatcaag agaaatcacc aagtctatgg aacaaaaata tgaatggaaa
     2521 gatacaacta tatttacagt attaaaaaga ttactaaaga agagagtttt tagaaacgga
     2581 gaaaatagat aagtgtacac actataatgt attagtaaaa gaaaaataat atcagagatt
     2641 ttaaacaaaa gaatttttaa agaatacaca taaaaattca atattaagtt taatgtcagc
     2701 actatgtagt aaggatgatg aagtagatgg tgatatttta aatttattag aagaatattt
     2761 taagaatttg aaataagaaa atgacaaatt gatacataat aaataaaaag aaggta
//
LOCUS       DAELJO010000327         2781 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.329, whole genome shotgun sequence.
ACCESSION   DAELJO010000327 DAELJO010000000
VERSION     DAELJO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.329"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            100..468
                     /locus_tag="KPB88_003808"
     CDS             100..468
                     /locus_tag="KPB88_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0056037.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            471..842
                     /locus_tag="KPB88_003809"
     CDS             471..842
                     /locus_tag="KPB88_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG0056038.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(987..1442)
                     /locus_tag="KPB88_003810"
     CDS             complement(987..1442)
                     /locus_tag="KPB88_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056039.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1481..2746)
                     /locus_tag="KPB88_003811"
     CDS             complement(1481..2746)
                     /locus_tag="KPB88_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG0056040.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1027 a          401 c          386 g          967 t
ORIGIN      
        1 atttataata tactccaaaa tttaaaaata atggttgaaa aatctaatta agagtactat
       61 aatttaatta agtaattact taatcaaaac gaggtactta tggaaaactt ggttaaaata
      121 tttaaagcat tgagtgatga aactagactt aacatactta tattagtatc caagagaaat
      181 atatgtcaaa aggggatttc taaatatcta gggatttcag attctgctgt ttcacaacat
      241 ataaaaatat taaaagatgt tgggataata acaggatata aagaaggata ctatgtttta
      301 tatcatatta ataaagaatc atttaatata tgtgtagatt ttataaattc tatgttaagt
      361 aattctagtg aaagctttat agatgtattt gatgtaaata caatacatct aggatgcagt
      421 aaagaatgta aatctataaa aaaatgttgt aagagaaggg agaaataata atgaaagtat
      481 gtataccagt agaggagaat aaaggattgg atagtaaacc atatggacat tttggttctg
      541 ctccaatttt tgttgtgtgt gatttagaaa gtggagaagt taaatcttta gataatggag
      601 atttagatca tgaacatgga aaatgtcaac cattaaaagc attgtcaggg acagctgtag
      661 atgcagtagt tgttggagga ataggacaag gagctataat taaattaaat ggaatgggaa
      721 taaaagttta cagagcagaa ggtgatacta taagtgctaa tttagactta cttaaaaatg
      781 gaaaattagt agaatttcca tcagaccata cttgctcaca tgacggatgt ggacatcatt
      841 aaaaaaaaac agctgatgaa atatatactt catcagctat tttttctttt aataaatacg
      901 attttattct tttaaaacca aaatttagat ttttatattt tttgtagtat ccttaataat
      961 atttcaagtt tacaagttta ctacttttag attttttttg tatgtttttc tatatagtca
     1021 atatcattta atatgtcatt gagttcttta ttaagtgttt ttaaatcatc accatctaca
     1081 ttgtagttta aaaatagttc ctctacatta tcaatgatag aattgacttt tctagtaact
     1141 tcaacaaatg tcttaaagtt actactattt ttagcattta gaaatttttt taaatccttt
     1201 gatataaatg atactatttc atattcatca ttaaatagac ctctaccaat aggatatggt
     1261 tcaattctct gtaaaaaata ttggtcttta gcattaatat tataagtata agatatttcc
     1321 caaccgtctc ctaaatcaaa cttgataaaa tataatgtac tacttacttt tgatattctt
     1381 gtggcaccaa gctgtcttaa aagaacttca atcgaatcac cttgaacagt attaatattc
     1441 atatatacac atccccttta gtattattat actatatttt ttaaaatttt ataattaaat
     1501 aaacagtaga tataataata gatataatca taagtggaaa acctattttg aaatactcct
     1561 taaaagagat aggatgaccg tgtttattac ctatacctgc tagtacaaca ttagcagatg
     1621 ctcctattag tgttccattt cctccaagac aagctccaag agatgttgcc caccatagtg
     1681 gcattacatc aataccttca gcttgcatag tcaaaataag aggaattagt gttgcaacaa
     1741 atggaatatt atctaagaaa gaagagacta tagctgacaa ccataatatc aacaacattg
     1801 taaatacaag atgtccttct gtaagtccaa tcaatgcttg agccaatttg tttattatcc
     1861 caacttctac taatccacca actactacaa ataatcccat aaagaataaa attgtagacc
     1921 attcaatacc tgccataatt tcatctacat cttgttttcc tattaaaagc attatagatg
     1981 ctcctgtcag tgctacaaca gaagaatcta tgtggattgt actgtgaaac ataaaaccaa
     2041 aaagtatgaa tgctattaat ataaggcttt ttataagtag aggtttatct ttaacagatt
     2101 ttttttcatc tagttttaat attgcatttt tagctctttc attcacaaca agttcttttc
     2161 cataaataaa tctaaagcat attatagtta tagcaagtat tactataaca gcaggaccta
     2221 aatttattac aaaatccata aaactaagat tggcagcact accaatcata atatttggag
     2281 ggtctcctat aagagtagct gttcctccta tatttgaagc taaaatctgt gttattaaaa
     2341 atggaactgg attaagtcct aatatttgtg taataactat agtcattggt ccaataagta
     2401 agacggttgt aacattatct aatacagctg ataaaatagc tgttataata gcaaagcaaa
     2461 tcattatctt ccatgggtca cccttagctt tctttgcagt ccagatagct atatattcaa
     2521 ataaccccga gtttttgaca acagctacaa atagcatcat accaactaat acaccaatag
     2581 tattgaaatc tatatggctt aatccttcat caagtgtaag tatattgaat attatcatta
     2641 atagtgcacc accaacagct gctacggatc tgtttacctt ttctgtaata atagctgcca
     2701 taactattaa aaatatacat atagctatta tttgttgaaa ttccataata taagtacctc
     2761 catttgtatt attttgtgcc t
//
LOCUS       DAELJO010000328         2778 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.330, whole genome shotgun sequence.
ACCESSION   DAELJO010000328 DAELJO010000000
VERSION     DAELJO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.330"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..392
                     /locus_tag="KPB88_003812"
     CDS             <1..392
                     /locus_tag="KPB88_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056041.1"
                     /translation="STQTITIDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENI
                     VPNFKIVDDNSIEIRSEVKVELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIED
                     RLVNIEEKLSGGLSNIATSVNELITYC"
     gene            407..1711
                     /locus_tag="KPB88_003813"
                     /pseudo
     CDS             407..1711
                     /locus_tag="KPB88_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2089..2382
                     /locus_tag="KPB88_003814"
     CDS             2089..2382
                     /locus_tag="KPB88_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056042.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILQHDTITELHCIDVEYGS
                     IDYTKNRIIGINIETKEPILEEIPIFVSEEEKRIQELENQLLLNENEKVGGLL"
     gene            2382..2564
                     /locus_tag="KPB88_003815"
     CDS             2382..2564
                     /locus_tag="KPB88_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056043.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGAEPIK"
     gene            2606..>2778
                     /locus_tag="KPB88_003816"
     CDS             2606..>2778
                     /locus_tag="KPB88_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBG0056044.1"
                     /translation="MNEELMKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGV"
BASE COUNT         1053 a          322 c          573 g          830 t
ORIGIN      
        1 catctactca aacaataaca attgacgatt gggttgagga tgcagaaaat ggattcaaag
       61 caactgtaac acatagttta ttaacacaga gaatagttgt aaatattata gatgctacta
      121 caaaagaaaa tatagttcca aattttaaaa tagttgatga taattcaatt gagattagaa
      181 gtgaagtaaa agttgagtta aatgtttatg ttattaatgg aaatgcagaa actcatttta
      241 ttaatgcaac tgtagatgat aacagagtat ctgaaatgac tacttattca tctaagaaaa
      301 tcgaagatag attggttaat atagaagaaa aattaagtgg tgggttatct aacattgcaa
      361 caagtgtaaa tgaattgata acttattgtt aaaggagagt gagaaaatgc agacagaatg
      421 gaactttggg tacaatggtt cgccacaaag tgttatattg aaacctggca aatataaatt
      481 tgaatgctgg ggttcttctg gaggtatcaa caattcttct tggtatactg atgctaaagg
      541 tggatattcc aagggtgaaa ttacattaaa aaaacaaact acattatata tttatgtcgg
      601 cgaaagtggt tatgcttctt catctacagc taataacact aaaagtggtt ttaatggtgg
      661 cggtaaaggt tacttaaatc aacaggttat gggtacttat tattctatgt acggtggtgg
      721 tgctactgat ataaggctcg ttggtggtgc ttgggatgat gagcaaggtt tgctatctcg
      781 tataattgtc gcaggtggtg gcggaggttc atatcatcct tacactggtg gtgcaggagg
      841 aggattagaa ggaggtactg ggtatagttc taatgacaga taccgtcccg gtggtactca
      901 atatcaaggt ggtattggtc gtgtaaatac agaaaacgga agttttggaa aagggtgttc
      961 tgttaaagat tcaactggcg aaggcggcgg aggtggctgg tttggtggtg caggaatgaa
     1021 tggtgtggga gcaggtggag gtggaagtgg ctacgtatta actaaagata gttataagcc
     1081 tactggctac acaccaacat ctgaatatta ttttgataat gttgttatgg aatctggtgg
     1141 aaatactgct ggtgcttatg gttatgctaa aataacatta ctacaagcat taccattttt
     1201 aacagtatct tcttataatt ccattacagc tacatttaaa gctgaccaca cagaccctgc
     1261 attactaact aaaatagaag tatttataga tgatgtatta aaagaaacta taacaacaga
     1321 tttaactctt gaaaaaacaa ttaactatac attagaagat aatgctctac acacacttaa
     1381 aatagtcgtt acagacagta ataatgctac agcagaaaaa gtgttaagta taagtaagaa
     1441 tataatgcca ctacccgaag atgtgaattt aaatgatata tcaacaaaat taacagaagt
     1501 taatgcaggg tttagaactg gtaaaacaag tattataaac actttagcat taaagaatat
     1561 agaagcaagt ttgaataaca cacttgtaga attatcagag aaaattaaaa caagttttga
     1621 tagttcggac gctagtgtac aagagttgca aaataggata acagaattaa ctaatcaact
     1681 aagccaaaga aaaagatggg ctagtggtac ttatactttt acccaaagtg atgttgctga
     1741 ctttgttgca aaagttggga aattatctta tttaactgta caaactaatt tgaattttac
     1801 tccaaaaata attgtagtac gtggtataaa attaaaaaaa acggctagtg tttttcttga
     1861 tttttctgat attactaatt ttggtgattt tggtacaaca ggacattatt attttaatgg
     1921 acgtcattat tatgattttt gtattaaatt tgaaaatttc tctagtaatt cttttcaact
     1981 tcgattatat agcatggaag gtttggaggg ggatggaata actagtgttt gtgtacttgg
     2041 tgatacgttt acatggtatg cttatgaata agaaaggtgg taactaaaat gaatagagca
     2101 aatagaataa tttacgacca aacaggtaaa atactcttgc aaacaggaga agcaacagga
     2161 gatatattgc aacatgatac aataactgaa ttacattgta ttgatgttga gtatggaagt
     2221 atagactata ctaaaaatag aattataggt ataaatatag aaacgaaaga accaattttg
     2281 gaagaaatac caatattcgt ttcagaagaa gaaaagagaa tacaagagtt agaaaaccaa
     2341 ttattactaa atgaaaatga aaaagtagga ggattattat aatgaatata aataatgttg
     2401 tagtaagaat attagcagag aggattttaa gtagaggctt aaatccttta aaaaatcgag
     2461 aatttgaatt agatgatgta actaacacag agtatagaaa agcagtagag gattatatta
     2521 tagagcatag tggagtagta gaaggagcag aaccaataaa ataggtaatg ttcctttttt
     2581 taatacaaag caataggagg ttttcatgaa tgaagaactt atgaaggaaa aaataagtac
     2641 acttgaaaca agagtaaaag agcatggtaa acaaattgat agaatagaga ttgaacaagc
     2701 aaagtttgct atacaaatac aaaacctatg tagtgatata aagaatttaa ccggagtact
     2761 taagtggctt gtaggagt
//
LOCUS       DAELJO010000329         2715 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.331, whole genome shotgun sequence.
ACCESSION   DAELJO010000329 DAELJO010000000
VERSION     DAELJO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.331"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            402..1676
                     /locus_tag="KPB88_003817"
     CDS             402..1676
                     /locus_tag="KPB88_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0056045.1"
                     /translation="MQFIDVSKYIEGANLKVKTKNGEAVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKVENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERQLFLYAKEEMSKIPNIVIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1883..2200)
                     /gene="trxA"
                     /locus_tag="KPB88_003818"
     CDS             complement(1883..2200)
                     /gene="trxA"
                     /locus_tag="KPB88_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0056046.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2467..>2715
                     /locus_tag="KPB88_003819"
     CDS             2467..>2715
                     /locus_tag="KPB88_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0056047.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKK"
BASE COUNT         1034 a          379 c          340 g          962 t
ORIGIN      
        1 taaaatcttt ttttctaatt atatacaata tattttccat tatgttctaa tcaatttctt
       61 tactattttt acaactttat tagtcatatt cataattatt tctaattagt gtttaacatt
      121 ttatgtaaaa tttataaaaa aatgtatata aattaaaata agtgactata ctaaaaatat
      181 gatacggata aacaataagt tcaacctatc tcccccaaaa tcaagtgagc tacagtttcc
      241 cccaactgta gctcattttc gtgtaattta ataaacatct caacaagaat taattaacaa
      301 aaccccttat aaaaacataa catatattaa acaagctctt gattaaaact tgagcttttt
      361 taataaaaaa cagaatagtt tattagataa ggggtttttt tatgcaattc atagatgtat
      421 ctaaatacat tgaaggtgca aaccttaaag ttaaaactaa aaatggtgaa gctgttaaaa
      481 gaatatattt taacaactct gccactccat tagttttaaa aaacgtagtg gataacttaa
      541 actgtgagat tccttggctt acttatatta atgctccagg aataatctca gaaaaaaata
      601 ctttaaaata tgaaaatgta cgctctacta tcctcaaact tattgatgga gacgaagaaa
      661 aggattctgt tatttatgtc aaaaatgcta cagaaggaat aaacttctta gcaaaatttt
      721 ttaaagttga aaatccagat aaatttgtta ttaccacagc aatggagcat atggctaatt
      781 acctgccttt taaagttaat tttcctacaa aagttgttgg aattacagaa aaaggagaac
      841 ttgatttatg tcaattagag aatatactta aaaattatgc tggtaatgta tctctcgtta
      901 cagttacagg agctagtaat gtcacaggta taaccactcc tatatatgaa atagcaagaa
      961 tagctcataa gtatggtgca aagatattgg tggatattgt gcaagtaatt cagcataaac
     1021 ctttttcaat gatgccacat gaatgtgatg agcatataga ttttgttgtt tttagtgctc
     1081 ataaatgtta ttctccacta aatggaggag ctctggtagg acctaaaaaa ttctttgaaa
     1141 aatttaatcc tgtactatat ggctcaggtt ctaccaaatt tgtaaatgat aacaaaatac
     1201 tatatgcaaa cgtaccccaa cgttttgaag caggataccc agagtatttt ggaactcttg
     1261 caatgggaaa agcactgaac actttaaata aaattggtct aaacagaatt tcaaaatacg
     1321 aaagacaact atttttatat gctaaagaag aaatgagtaa aatacctaat atagtaatat
     1381 atggagataa atcaaataat gtaattatcc caaatatatc ttttaatata agtaatatgt
     1441 attacaaaga tgtagcaaaa tatttagttg ataactttgg aatagaaact ggagctggag
     1501 tagtaggtgc agatatttat gtacaaaaat tattaggtat aagtccaaaa gaagcatacg
     1561 tcagatttat gagggaaaat cctgtaggtc ttgttaggat aagcttagga atgtacaata
     1621 cttttgatga ggtggatagc tttatacatg ctattaaaac tttggctttt aaataagctt
     1681 tttctattct tttttatcaa taaatagaga gtagttttgt tttcacaaaa aaacaaatta
     1741 tatcaacagt caattttatt agcaattaga cactcatttg taactataca aactatttta
     1801 cactatacaa acaaaaaact cattacaatg agtttttttg tttatatatg agatttattt
     1861 taattacttc aatttacttt aattacatat atgattcaat tttgtttttt aaattctcct
     1921 taggcataaa acctattaat ctatctacag gttttccatc cttaaatatt agcatagttg
     1981 gtactgtaga tacttcaaat ttctgagcta attctatact ttggtctatg tccaccttca
     2041 agaagtttgc tttttcaacc atctcatttc ctaaagcttc atatattggt gatagcatct
     2101 tacaaggtcc acaccaagtc gcaaagaaat ctacaactac taaaccatct ttattttcta
     2161 cttcatttat aaattcatta ttatttatta tttttaccat aatatacatc tcctttatta
     2221 tcttcttgat attaatatac tacgccctat ataacagttc aattaggcac ttttttgtaa
     2281 ccgcctattt ttaatagatt taatattatg ataaatagat ttataagatt aaatatttat
     2341 gagactaaat atttctaata tattttattt ttttcttaat atagttgaca ttttttccct
     2401 taaattataa aatcaaatta ataactacat aggtagttac taatctttta tcaaaggagt
     2461 ttctatatgg ataatataat taatgaatta caaaatatag gatttactaa atacgaatct
     2521 caaatataca tatcattatt aaaggaaagc cctttaacag gatatgaaat aagtaaatta
     2581 tcaggtgtac cacaatcaaa agtctatgaa aatataacaa agctattaaa caataatata
     2641 attatcaatg ttggaactga ccctattaag tacataccaa taaaccctaa tgaattatta
     2701 aaaaataaga aaaaa
//
LOCUS       DAELJO010000330         2715 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.332, whole genome shotgun sequence.
ACCESSION   DAELJO010000330 DAELJO010000000
VERSION     DAELJO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.332"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            82..396
                     /locus_tag="KPB88_003820"
     CDS             82..396
                     /locus_tag="KPB88_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056048.1"
                     /translation="MESLKRIKKMVQKQLVLAELEINKNSKLYEELENKDRGLIDDIH
                     MREYLREKVAWERVKYAIENILGGINLEIKSKEHEESEDYKIFQLILEELERDKPIDV
                     QI"
     gene            480..989
                     /locus_tag="KPB88_003821"
     CDS             480..989
                     /locus_tag="KPB88_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG0056049.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNIIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            999..1607
                     /locus_tag="KPB88_003822"
     CDS             999..1607
                     /locus_tag="KPB88_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG0056050.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            1617..2498
                     /locus_tag="KPB88_003823"
     CDS             1617..2498
                     /locus_tag="KPB88_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0056051.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAISNGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNNKAVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            2558..>2715
                     /locus_tag="KPB88_003824"
     CDS             2558..>2715
                     /locus_tag="KPB88_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG0056052.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTE"
BASE COUNT         1205 a          259 c          527 g          724 t
ORIGIN      
        1 tgctttaata atggtaggtt tatttgcaat atgtttagta ggattagttc aaaatagaga
       61 ttaaattaag ggggattaat tatggaaagt ttaaaaagaa taaaaaaaat ggttcaaaaa
      121 caattagttt tagctgaatt agagataaac aaaaatagca aactttatga ggaacttgaa
      181 aataaggata gaggtttaat agatgatata cacatgagag aatatctaag agaaaaagtt
      241 gcatgggaaa gagtaaaata tgctattgaa aatattttag gtggtataaa tttagagatt
      301 aaatcaaagg aacatgaaga aagtgaggat tacaagatat ttcaattaat tttagaagaa
      361 cttgaaagag ataaacctat agatgttcag atataagaaa agagccattg ccgtggctca
      421 attcaaataa atatatcaaa atttaattaa gctaattata gcacaaacgg agggaaatta
      481 tgagtacttt atatgaatta actacagatt tattagaaat agaagaaggt ttaacagaaa
      541 caacaggaaa tgaaactgaa aaactagagg aaataaaaga aataataaaa caagagatac
      601 aaaataaaaa cactaggata gtttcagtaa tattaaacat tgacagtgat ataaactcta
      661 tagattcaga gattaaaaga ctgcaagagt taaaaagggt caaaaagaat actcttgata
      721 gattaaaaag caatataaaa gactgtatgg aattacttgg tactaaaaaa gtagaaacag
      781 ttttaggaaa tataagtata agaaagtcag caggtagctt agtcatagaa gatgaagaaa
      841 agatacctgc tatatataaa acagtagagc aagttgtaaa agtagataag aatatcatta
      901 aagactttat taaaaaaggt catgaggttg aaggttgcag gattgaatat ggaactacac
      961 taacaattcc aaaagctaaa aaagagtagg tgaggaccat ggaaactaat aatgtttata
     1021 taaaacttgt aaatatacag agtactttaa aagctcctaa aagtcaattt aatagctttg
     1081 gtaaatacaa ctataggagt tgtgaggata tactagaagg tttaaaacct attctaaagg
     1141 aagaaaaagc attagttata ttggatgata atattgttca gataggaaat agattttatg
     1201 tagaagctac agcaacttta atagatgcag aaacaggaga gaaagtatct acaaaggcat
     1261 tagctagaga agatgaaact aaaaaaggta tggatttagc acaagtaact ggaagtgtat
     1321 caagttatgc aagaaagtac gctttaaatg gattattttg tattgatgat acaaaagata
     1381 gtgatgcaac aaataaacat ggaaatgagc agaaaaaaaa agaagttaat gagagtgaat
     1441 taaatacact atattcgcta ggagaatcta tagaaaaaga taaaaataga gttgatagtg
     1501 aagtatataa gaagtttgga aagttagcag tagatttgac taagcaagag tatgagaaag
     1561 ttttaaatgg atataagagc attttagaga agcaaaaaca agagtaggtg atagtattgg
     1621 ggattataag agtaagcaaa gacaaagata atccatatgt agttttaaat aaaacttgtt
     1681 tggaagatgt aaaattaagc tggcaagcaa aaggtttaca ttcatatctg attagtaagc
     1741 ccgaccactg gaaaatctat gttaatgacc tttgcaagag aagtaaaaat ggaagagatg
     1801 ctacagcaaa tattttaaaa gaacttatag aaaatggata tataacaaga aaaccttgta
     1861 gagattctaa tactaataaa atgcttggag ggtatgatta tgaagtatat gagataccac
     1921 ttgaaaatcc tcagaagcta aaatcccgaa aaactgattt cccggaaacc ggatttcccg
     1981 gaaaccgggt ttctcggaaa ccggaaaaca cggaagtagt aagtaatgac tttaaagtaa
     2041 ataatgatat tactactatt gttattaatg aacaatccaa taaagacaaa accacctata
     2101 taaaaaaata ctttgaaaaa tatataggtg tgattactcc taataacttt atagagttaa
     2161 tgagttactt agatgatgga atggaagctg atgtaattat aagagctatt gatgaagcaa
     2221 tatcaaatgg agttaagaat tataagtatg taaagacaat attaaacaat tggatagaag
     2281 caggtgtaaa aactaattta gaacttacag agtatcaaaa tgagtttgag aggaagaaaa
     2341 agagtaaaca ggagaagaaa cagtctaata ataaagctgt gaatactcat aatgtgaata
     2401 aaaataaatt tgctaacttc aatcagactt tcactcaata tgaagaaaaa gagctagatg
     2461 agattattaa aaagagtcag aaggaaaaat ttaaataaaa ttaaacttct aggaagtaaa
     2521 tatcaatata ttgtttcctg gaaaagggag gtataaaatg gcgagaatat atgcacaaag
     2581 aagtggttct ttaaatgagc aggatagatt ggaattgtta agattacttg ggaaagctgg
     2641 atatacagta aagattgcta gagagaagca aaatagcaag acaacttata cttactttgt
     2701 tgagtataca gaaga
//
LOCUS       DAELJO010000331         2655 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.333, whole genome shotgun sequence.
ACCESSION   DAELJO010000331 DAELJO010000000
VERSION     DAELJO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.333"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..560)
                     /locus_tag="KPB88_003825"
     CDS             complement(<1..560)
                     /locus_tag="KPB88_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0056053.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTEL"
     gene            complement(572..1189)
                     /locus_tag="KPB88_003826"
     CDS             complement(572..1189)
                     /locus_tag="KPB88_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG0056054.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(1182..2231)
                     /locus_tag="KPB88_003827"
     CDS             complement(1182..2231)
                     /locus_tag="KPB88_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0056055.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            complement(2232..2651)
                     /locus_tag="KPB88_003828"
     CDS             complement(2232..2651)
                     /locus_tag="KPB88_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0056056.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
BASE COUNT          774 a          510 c          283 g         1088 t
ORIGIN      
        1 tttaactctg ttttagcagt ttctaaatta tttgttaatt ctgttttagt tgtatctatt
       61 ttagtattaa cagtacctat tttagtttct aagtcttgta tatctttgag tgttgcaaag
      121 attattgttg ggtcaatttt aagttctata ttatttacat tagatacaat aagcacagtt
      181 ttaaccttca tgtctaccac tgcaccttgt tctatagaag gtttataaca ctctttgtat
      241 ttagaaatgg caattaaatt attttcatca tctaaatatc ctatttctct tatcataaat
      301 ccgcctacac ttgatggtat taaactctct aatattatac aatttggtgc agtttcatct
      361 gtagttgtat ttccaatatt gccttcccat accacgtttt tgagagctgt ctgactctca
      421 gttggagtat attcactccc tcctccatca ccaagttgaa tttttacaaa tcccacttta
      481 ttacctgtga cacttgcatt tgctatcttt gcttttccta catctgtaat tatagtgtaa
      541 taacttttat ctatagccaa tatatcacct cctaaaatat tgttatctct tggtatccaa
      601 ctccattgcc agttaataca tcaatttctc cataagtttc tatatctggt ggactccaag
      661 ggtatatagt tatttcttga cccattaggg ttgttatacc aaaattcata taattgtctt
      721 tgcttataag cactctagtg taatctaaag tcatattaca tggcttaata ttacttacaa
      781 aagaatgaac ttcctcaaac caatcttgat ttctagcatc actttcaaga tgtatattat
      841 aagtagcatt attaatagtt aactcataat taccttctcc aactatacta tctagccaat
      901 tccttaaaaa tctctctgag taaggtaatt tacttatata tttactaaaa atcctaaacc
      961 ttctatcttc taaactctca ttacttttag gagttataga cattatcttt tcccatcttt
     1021 ttataccact tatagttagg tcctctaaaa actggtcatt tgataggtcc tttaatttat
     1081 catgtaatat ttttatttct ttatttccta cattaaatac ttttatatat tcttctttat
     1141 cttgtagaat ttgtggtaag taatttatta gattaatctc tttatccaac tacctcacct
     1201 ctcactacta tactgttact atttattgtt agattagatt taacctcatt tatcattgta
     1261 tttgcaatgt ctaatactcc atcaatacta agtaatctag tttcaatttg agatatacgg
     1321 actattaagt tttcttcatc ttcccaactc atgttaagtt catttaaata gtcgtctatt
     1381 gcttcttctg caattgattt tatattctcc caagtgtagc catttttgta tgttatctct
     1441 gctgatatat ttatagttgt acttacaaca cctgtaacag taactttatg ccctattggt
     1501 gctaatccta agccttgtcc ttgatgtcca attgggtcaa tttcttcttg cactaaatta
     1561 actaaatcct ctgatggtac tttgaaatta gagttaatta ttactaactt aacagttcct
     1621 ccaccgtccc acacaggata aaccttaact cctccaacat cttgtatttt gttaacttca
     1681 tctttataat tttgcatatt tccaccaaag ctctgtgaat ttaggctatc ataatatctt
     1741 tgtcttaaac tatcctcact ctcttcatct tctccattta tcagtatttc tgtcagttct
     1801 gctgtttcta atttgtctat atattcgata ggtattagtt gtcccaactc aaaaataggt
     1861 cccgcagtct cacatttcat cttatatata ccttcagata ttctctcaat tgctacatag
     1921 ttatattctc ctagattaaa cctagaatca ataggaatat ctatgttaaa aactcctttt
     1981 gcaattgtat tagtggcttc aagtggtgta atacctctct ctttacatct cttctctaaa
     2041 taataataac tagcagtatc tacgaatgtt tggtctagta attcatccat agcaatgtat
     2101 gtttctgtaa gttctatagc aacaggagca agagcattat atattataga accttccctt
     2161 ttatcaaaag tatctggtac actatctaac attcttttaa ttatattttc aaatgtcatt
     2221 aactcaaaca attatacact caccaccttc tctgctttta tatttccata ttttgtatga
     2281 actgaaaatc tacattgtac tttacccttt atattttgaa actcaaaatt atctatattt
     2341 tcaatcctat catcttgaat tagtgcttct gttatccttc tttcaagttc gggtattaca
     2401 tatgaaatag gttctccaat taaatcattt aattcaacac cataatttct agaataaata
     2461 aggtgctcat acctttcagt atttaaaatt aaaaaaatgg tttgttttaa tgcttctaca
     2521 tcatcacaaa taccatctac tctatttttc tctatattca atttaaacgt cttacttggt
     2581 tcttgtctaa catcaaaatt aattatcgat acatcttcta tatcatagtc aatgttatct
     2641 gttggtaaca tttca
//
LOCUS       DAELJO010000332         2639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.334, whole genome shotgun sequence.
ACCESSION   DAELJO010000332 DAELJO010000000
VERSION     DAELJO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.334"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..427)
                     /locus_tag="KPB88_003829"
     CDS             complement(<1..427)
                     /locus_tag="KPB88_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056057.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDA"
     gene            complement(655..1074)
                     /locus_tag="KPB88_003830"
     CDS             complement(655..1074)
                     /locus_tag="KPB88_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056058.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            1373..1582
                     /locus_tag="KPB88_003831"
     CDS             1373..1582
                     /locus_tag="KPB88_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056059.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1614..1823
                     /locus_tag="KPB88_003832"
     CDS             1614..1823
                     /locus_tag="KPB88_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056060.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(2040..2438)
                     /locus_tag="KPB88_003833"
     CDS             complement(2040..2438)
                     /locus_tag="KPB88_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0056061.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          977 a          337 c          324 g         1001 t
ORIGIN      
        1 tagcatctgg attataactt atctgaacca tttgattttt cttaacatgt tcaaggtctt
       61 caccaccata agctatttgt aacttaactg gtaacttacc ttgttttata atagcaacgt
      121 actctttttt accttttttc tctaaactaa tcaaattgcc aacataaggt ttaaagttct
      181 gatacttttt actagaattt cttatgtaga agaaagcacc aacagcaata actaaattta
      241 tgccaagtgt aacccaagaa ttgattttaa gcatagctcc agcgattatt atcacgaaca
      301 ttaaaacgat aggtaatata gctttcttaa gaagcaattt acccattatt tcattagcct
      361 ttttctcagg gccactcata gtttttgatc tagcaaatga ttgcgcgaat ttgtctctta
      421 agcccatttt atcctcctaa ttttaataaa tatttagtta taataacgag atattacttg
      481 aaactaaaaa tttactacat ttatattatg tttgactttt gtataaataa ttacattcaa
      541 gtaaagcaaa atatactaat tattttatca taaaattata aaaaagaaaa taaatgaaat
      601 aaaaatatta gaacaaagaa atgatgtaaa atcgtatcaa aagcaacata aaaattattt
      661 atctattttc tcatctttat ttttgttata ctcaattttt cctaaatcct tctctttttc
      721 atattcatga agttttaatt caatcatacc ttctattttg gctttatcat aatcatttaa
      781 ctttctaaag ttgtttaaaa gttttatttc attagagttt atgctattaa gtggatagtt
      841 tgaggaggaa tcgcaaatta aatctgattt atgtgataga ttatctccat ttaatagcca
      901 gtctactgaa acattaaata tctcagctat agattttaat atttcataat ttggttttct
      961 aatgtttctc tcaaatttac ttaagttgtc acagcccaac atttcttcta gttcatattg
     1021 tttaaggttt tttgcttttc tcaaataaac aattctttct cctaaagtat ccataaacac
     1081 tctccattca attaatgtca aaaagacttt ttaagatgta aatagtttca aattaaaggt
     1141 caaaatgaca taaaaagcat tgacttaagg tcaaaatgac tttataatta acttaatgat
     1201 acgaatttac atcctaattt tagcacaaag taatcaaaaa atcttattta gtattaaata
     1261 aatttatata cttaatatgt gtacatatta aaaatatata ctaaatagag ggggtgcgta
     1321 agctaaaata atataaaagt aaatataaat cacttagaaa ggaagttgat aaatggatgc
     1381 tcgaaaaaaa tggatacctt ttttgggagt gcaagtcaag caaagactta ttgaattaaa
     1441 tatgactcaa agggaattag cgaagaaaat aggtgttaat gaaaactatt tgtcagctat
     1501 tttaaatgga agaagaacag gtaaaaaata taaatcatca atttatcaat tacttaatat
     1561 agaatattca gaagatgatt aataaatagt atataaagta ggtgaatatt cttgtgtgta
     1621 aattggattc agatggggtt atagagtgtt gtagagcaat tgatgatttt attacagcac
     1681 tcagtaatat aaaaagctta aatatggaaa gattaaatac tttaactaaa tattctagta
     1741 catgttcaat ccttcttaaa gaggggaatt atgaaggatg tacaattgtg tatagaaaga
     1801 tgttggaaga attaaaaaca tgagtaatgc atttcttagg aatataagtt atacatagag
     1861 atgtattata tttttcaaag tacttaaact aaaatatgga taagataatc taaatattat
     1921 aaatgtgctt gaaattagac tatacttgtt tttaaataat ccaatatcca tattttagta
     1981 atatactaca aaaaaagaag gttaatagat gatgtaaaat cgtatcaaat tatgtatgtt
     2041 taaaccattt tatcttcatt attattagag gaatgctttt ttaagtcttt atattcagat
     2101 atcttaagtt caagtattcc ttctattttt attttatcac gttcgtttag ttgtctgtat
     2161 agatttaata tcgtcatttc atcattagta acatgtaagt aatcttcttt atcttctttt
     2221 acactactat tgacatttac cttctcttta ccatagagaa gccagtcagt cgtaacatta
     2281 aaataatcag ctattgacat tagtatatca caattaggtt ttctatctcc tgtttcatac
     2341 ttgcctaagt tttcaaattt taaaatatcc ataagtttgc gctgagtaag ttttttggag
     2401 tttctcaaat aagcaattct ttttcctaaa gtatccacaa aatacactcc tttcttttta
     2461 tgagtaatgt ctaaatgaca tttaaaatta aaaatatata aatttataat ataaaactac
     2521 taaattaaag tctaaatgac attttgctta aattaatatg ctcataatat gattttaaca
     2581 tattatagtt gaaaatatat ggtttatttt gatttgtata tataacaata ggtttaatt
//
LOCUS       DAELJO010000333         2637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.335, whole genome shotgun sequence.
ACCESSION   DAELJO010000333 DAELJO010000000
VERSION     DAELJO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.335"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            211..1392
                     /locus_tag="KPB88_003834"
     CDS             211..1392
                     /locus_tag="KPB88_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0056062.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVEDIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1534..2451)
                     /locus_tag="KPB88_003835"
     CDS             complement(1534..2451)
                     /locus_tag="KPB88_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056063.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1019 a          322 c          423 g          873 t
ORIGIN      
        1 aaaaagatta tctttatttt aagaaagaca tttatttata attatataca aaaatatttt
       61 ttaatgtttt tgtgtataat tataataatt taatttatat taatgcactt aaatttgaaa
      121 tagaatgctt atagtggtaa aattaattat tagaatatta gaaataataa ataaataata
      181 cattgtactt aaaaggggga gaaatttaag atgaacaaaa aatatattaa agcagggaat
      241 ttaattgatg gaaccggaaa tagtattatg caaaataagg gtataattat tgatggagat
      301 actatagttg aaattgaaga tataagagat gatttaaatg gatatgatgt gtacgattac
      361 ttagataaaa ctgttatgcc aggcattatg aattgtcatg ttcacttgac aatggagcca
      421 gttggaaatc caaagatata ttatgataat gtatcagatg tagagcttgt agtaaatacg
      481 ataaaacaac ttgatgctta tttggattca ggagtaacat atattagaag cttaggttgt
      541 cccaaatatg tggatgtaca acttaaaaat cttatagaca aagggtctat agatggtcct
      601 ggtatagtta cttcaggtcc agttatatgt atgacaggag ggcatggata ttattttggt
      661 attgaaagtg atggagtgga tgaatgtaga aaatcagcca gaacagtttt aaaaaatgga
      721 gtagattgtg taaagataat ggcaactgga ggtgtaacaa ctgatggagt tgagccaggg
      781 tcaccacaat taactttaga ggaaatgaag gctgctgtag atgaagctat aaaagcaggt
      841 aaaataactg ccacacatgc acaaggaaga actggtataa aaaatgcagt gcttgcagga
      901 attacttcta tagaacatgg tgtttattta gatgatgaaa taattgattt gatgcttgaa
      961 agaggaactt acttagttcc aactgtggct gcaccatatt ttattctaaa tgcaggaaaa
     1021 gaaagtggaa taactgaagc tactcttaga aagtgtgaga ttgttgccat tccacacaaa
     1081 gagtcattcc taaaagcata taaaaaagga attaaaattg cagttggttc tgatgcagga
     1141 acttcttata atgaacatgg aaaaacatat tatgaaatga aactaatgtc agattatggt
     1201 atggataata tggatataat agttgctgct acaaaaacag catctgaact tttaagaatt
     1261 gataaaaatt atggaactct agaaaaagga aagaaagcag atgttatagt tgtaaatgga
     1321 aatccagtag aggatataga cgtacttgca gatgtgttag cagtatttaa acttggtaaa
     1381 aaagttagat aaatagtata aaaataaaaa agagtgtcta aagaaatttt taaataatta
     1441 tagacactct tttttattag taataattaa attttttata ctttagaaaa tagattctat
     1501 tttatattta gatataactc tttaaaatca ttcttatgca atataatcta tagaatctac
     1561 acatacaaag tatattcttg aatcattaga atttccaagt actataacat catttttctt
     1621 acctagaact tcaacaccag ttagaaccaa actaccagct actacagtaa cttgattatt
     1681 tatatttgac atataaagat tatctaggat acctttatta caacagcaat catcacaatc
     1741 gcagttacaa ttacattctt tgtaatgttt tttatcaaga tagtaaccta aagatgctat
     1801 gaaatcttca taatctgcag gtgtaatatc aaatgcaaca gctttaagat tacacaatga
     1861 tgctaaatct acgtttgcta atggagtaga tgcagcaagt ataaaatcaa ttatagcagc
     1921 aagtattgca tctaatatag ccccaaggtc tatcgtagga ggaataactg catcaataag
     1981 tgcaattaat tcagctactc ctggtatagt tcctatagta ttaattaatc cctcaagagt
     2041 taaagggact ggaataggat aagatactct accagctata tctattaaat cacagttaca
     2101 agcagaaaat ctttcaaaag taccatcaag tccagataaa ttatcttttg gaggagctga
     2161 aagatctata cctaccaaat tagcacctac tataaagaaa tctgagataa aagcaaattg
     2221 attaaagttt acaaaaggtc ttagagcatc atatcttaga agttctaagg cctctctcat
     2281 acttttttta caacaacatt gttttatttt aggttcttta tatttatttg gataattata
     2341 gtatctatca gctacttgat aattttcata gtatctttca tttgtatttg gataatcttc
     2401 ctcatattct tgtgtaaatc taaagtcctc tctacattta ttattttcca ctaaatacac
     2461 tccttttata ttttaattta aatgtataga taattaagct tatctatata gtaatattat
     2521 atttataatg taaaaaaatg ttacaaaaga tattataaac gaataaaaaa taataatttg
     2581 tcagataaac ttgtaaattt ttatgaataa tccattctaa aacaaaaaac tgtgtat
//
LOCUS       DAELJO010000334         2625 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.336, whole genome shotgun sequence.
ACCESSION   DAELJO010000334 DAELJO010000000
VERSION     DAELJO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.336"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            79..1890
                     /locus_tag="KPB88_003836"
     CDS             79..1890
                     /locus_tag="KPB88_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0056064.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     TSLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(1978..2559)
                     /locus_tag="KPB88_003837"
     CDS             complement(1978..2559)
                     /locus_tag="KPB88_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056065.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          970 a          291 c          361 g         1003 t
ORIGIN      
        1 ctgtaaaata ttgataatca ttttttataa gagattgatt atttgcaaat tatatatatt
       61 acaaatgagg taacaataat gaagaaaaaa tatgattttt taatttattc tgctatttgg
      121 ctagttatta ttattctttt tatattttct ctatatcaca gtatagagca tattaaaatt
      181 ataagtcaca ctggtactat tcgcagtgaa actcaaaagg ttgttaagca agaattgaat
      241 aatgaaagaa atgatgactt aataaaacga ctggataata ttctgataaa gctaagaaca
      301 ggtaatggag aaaatggatt tcaaagatgt gataataaag aatttcaaca aaaactaaat
      361 caaatggatt ctatgtggga atctatgaag aaagaaatta taaaagttag aaatggtgct
      421 tcaggtgata aattatataa attgagtgaa gagtattctg tgttgtctaa tcaaattgtt
      481 tttatatctg aaaaacactc taatgcaaaa ttgtattctt ttgctactgc actatttatt
      541 tatcttatat tctcaacaac aagtctttta atttgggaat attataataa aaaaaggttt
      601 aaaagaatat tttatactga taatttaaca aaaataaaaa atcaggttgc atttgaaaac
      661 agagcgattg aaattttgca caatgcatct aacaaagagt atgtactgct aaatattgat
      721 attgacaatt ttaaatatat taatgataca catggatatg aatatggaga taaagttcta
      781 ataatagttg cagctgcact ttctaaaact tttaatatca aagaaacttg tgcaagaatt
      841 ggttcagata attttgtaat acttgcaaaa tatagagatt ctcttttaga ggatattcgg
      901 gaaatgctaa ctaatgctat aatctctgaa ttagatatga atgtaactca aactatatct
      961 tattgtatag gagcgtactt agttgaaatt gataatttag gttataaatc catcaattct
     1021 atgatggata aggcaaatat tgcacacaag gtaagtaaaa caagaggaat ttcctccaca
     1081 gtatggtata atgaaaatct gttaaaacag ttacaaatgg aaaatagtat ttataattat
     1141 atgtataaag cgatagaaaa tgaagaattt catatgtatt tgcaacctaa atttcaaata
     1201 tcttctttaa atgttgtgag cgcagaagct cttgtaagat ggttttctcc agaacttggt
     1261 tttttatcac cagatgaatt tattccattg tttgaaaaaa gtggatttat tattgaattg
     1321 gacttttata tgttaaaaaa agcgtgttca tttgtaaaaa aaacgtttat gaaaaaaaat
     1381 caatatacat atccgattgc agttaatttt tcaagggtta ctatttacca aaatagtttt
     1441 tatcaaaggt ttttagatac tgttaaagaa tatgaaattc cttttaagta tatagagata
     1501 gaggtaactg aaagtgcttt taatgaaata tctcaaccag ttatttcgat tttagaggag
     1561 cttaaaaagt taggattttt aatttcaatg gatgattttg gatcaggata ctcttcatta
     1621 agtcttttat gttctctatc tattaatgga ttgaagttag ataaaagctt acttaaagaa
     1681 acatttaacc gggagaaagt atatagtatt attcagtgta ttattgaaat gtcacatcgt
     1741 ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa aagacttgga atttttaaat
     1801 acagttaaat gtgatgtagg acaagggttt tatttttcta aaccgataga agaaaaagaa
     1861 ttttttaaca aatatgtaac taaaaaataa tataattatt tttatcagtc tatacattga
     1921 gtcattttat atctcgttaa atgactcaat gtattgttat gtattttaaa atataaatta
     1981 tttaaaacgt tttttgtact tatatagaaa ttcttctata ttttttgatt gttctcttat
     2041 ttcaaccgct tctatatcta gtaaataaag tatagaatca tttgtaagtt caacatattc
     2101 accatcttct gaaccgagtg catgtctctc aatttttaat cctttttctt cataatattt
     2161 ttttgtttct atatatagtg attctcgctt ttgtatgtag tctttcactt cattatttgg
     2221 aaagttatat gaatccacta atgtttcgcc atttataaaa gttttctcgg tagcaacttt
     2281 ttcatcccaa caatggattt cttcccaagt aaaaaatatc tcagttccat ctggaatgaa
     2341 tgaaaacctg gtaataatat ataaataatt attgaaattt acttcataat attcaggaat
     2401 atcttcaaat tccatatagt aatttgtata attaaaatta ctattatctt tttttacaaa
     2461 gggtattttt tcaataaatt tatatattga ttgttcattt ttaaaaattc ctatatcata
     2521 acttttttca aattgattaa tttttaataa atacaacatt ctttctcctt ttattcaaaa
     2581 tataattttt ataaatttac taatctttag cttttttaat tatat
//
LOCUS       DAELJO010000335         2609 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.337, whole genome shotgun sequence.
ACCESSION   DAELJO010000335 DAELJO010000000
VERSION     DAELJO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.337"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            270..1115
                     /locus_tag="KPB88_003838"
     CDS             270..1115
                     /locus_tag="KPB88_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0056066.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVSLVSGLILGLLVSQGLLILLLKLFEFNMSEYSFVISIPAIIK
                     TIVYFGIIFLLVMIFNIYLISKYKMIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLF
                     TVYNTTNHIIINLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEY
                     IEKYK"
     gene            1370..2137
                     /locus_tag="KPB88_003839"
     CDS             1370..2137
                     /locus_tag="KPB88_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0056067.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
     gene            2127..>2609
                     /locus_tag="KPB88_003840"
     CDS             2127..>2609
                     /locus_tag="KPB88_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0056068.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIK
                     T"
BASE COUNT         1093 a          243 c          381 g          892 t
ORIGIN      
        1 ttgacattaa caattaaagg gaaaacaata taaatttgat tcaaaagcat caagattatt
       61 acttgagagt tttgaaagtt taaatcaaga tttaaaaaca acaattttaa tgctaactaa
      121 tgatgctttt atagctagtt atactcatag aaacttattt ataaaataca gaaaaatatt
      181 tatagagtta gcaagatgaa ctgattcaat aaaagagtct tttaatagaa taattgaaga
      241 aatagctata atcagaggag atgataacaa tgtattctaa aatagcgatt aaaaatgtta
      301 gaaaaagctt taaggattat agtatatatt ttttaacttt aacattagta atttgtattt
      361 tctatagttt taactctatg gaatctcaaa aagctttaat ggaaatgagt acttcaaaaa
      421 agcctgattt agaaatgttt aagaatatga tttcatatat atctgtgttc gtatcagcta
      481 ttttatgtac tttaattctt tatattaata acttattaat caaaaaaaga aataagaaac
      541 ttggaattta tatgacttta ggaatgggaa aaattgaaat gtcgaaaata ttagtttttg
      601 aaactataat agttggaata gtctctttgg taagtgggct aatacttgga ttattagtat
      661 ctcaagggtt attaatacta ttattaaagt tatttgaatt taatatgagt gaatatagct
      721 ttgtaatatc aataccagca attattaaaa ctattgtcta ttttggaata atatttttac
      781 ttgtaatgat atttaatatc tatttaattt ctaaatacaa aatgatagat ttattaattg
      841 tggaaagaaa aacagaggat ataaaattta aaaatccatt tgtatatatg attacatttg
      901 tatcatgctt tattttctta tttactgtat ataacactac aaatcatatc ataattaatc
      961 taatagatga taaacaatta aactccaagt ctaaaggtgt agaaataaat gaatttacat
     1021 atcaagattg gcaacaattt tgtgatataa agtattatgg aaagaaaggt ttaatatcaa
     1081 tgactttaaa agaatatata gaaaaataca agtagaataa aataaatttt agatggaagg
     1141 cattgccctc tatatttttg tgttttctag aattgagtaa attttgttac tctagcaaag
     1201 tataaaagaa agaatgctgg tcttgaatat tttatattag taattattag gtaataatat
     1261 ataattacta caaaaatgaa acattaatga aatgtaaatc aatttaaaaa aattaaataa
     1321 aaaattacaa tagagatata agaaaattaa tttaaaggag aaataggaca tgaagaatat
     1381 attaagtgtt gaaaaaatag aaaaatatta tggaaacaaa gataatatta caaaagcaat
     1441 agataatata agctttaaag ttgatgaagg agaatttgtt gggataatgg gtccaagtgg
     1501 tagtgggaag acaacacttc ttaactgtat atcaacaata gacaatgtaa caataggtaa
     1561 aataataata aataataatg atattacaag attaaaatct aaaaacttag ataagttcag
     1621 acaaaatgaa ttaggattta tattccaaga ttttaatctt ttagatactt taacagctta
     1681 tgaaaatata gcattggcat taacaattaa aggagaaaaa ccatctaaaa ttgattcaaa
     1741 aataagagat gtagctacat acttagaaat agaaaaagtg atagataaat atccttatca
     1801 aatgtcagga ggacaaaagc aaagagtagc atcagctaga gcaatagtta ctaatccatc
     1861 attgattttg gcagatgaac ctacaggagc acttgactca aaatcagcaa gattattact
     1921 tgagaggttt gaaagtctaa atcaagactt aaaagcaaca attttaatgg taactcatga
     1981 tgcttttaca gctagttatg cccatagaat cctatttata aaagacggaa aaatatttac
     2041 agaattagta agaggagctg attcaagaaa agagtttttt aatagaataa ttgaagtaac
     2101 aactctaata ggaggagatg ataacgatgt attctaaaat agcaattaaa aatgttagaa
     2161 aaagctttaa ggattatagc atatattttt tgactttaac attagcaatt tgtattttct
     2221 atagttttaa ctctatagaa tctcaaaaag ctttaatgga aatgaatgct tcaacaaaat
     2281 ctcatgtaga aatgcttaag aatatgattt catacatgtc tgtatttgta tcagttattt
     2341 tatgcagttt aattctttat gctaataact tcttagtcaa aaaaaggaat aaggaacttg
     2401 gaatttatat gactttaggg atggaaaaag ttaaaatatc gaaaatatta gtttttgaaa
     2461 ctataatagt tggaatagtt tctttagtaa gtggactaat acttgggtta ttagtatctc
     2521 aagggttatc aatattatta ttaaagttat ttgaatttaa tatgagcgaa tacaattttg
     2581 taatatcgat atcatcaatt attaaaact
//
LOCUS       DAELJO010000336         2603 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.338, whole genome shotgun sequence.
ACCESSION   DAELJO010000336 DAELJO010000000
VERSION     DAELJO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.338"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            225..1211
                     /locus_tag="KPB88_003841"
     CDS             225..1211
                     /locus_tag="KPB88_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0056069.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1380..1526
                     /locus_tag="KPB88_003842"
                     /pseudo
     CDS             1380..1526
                     /locus_tag="KPB88_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1548..>2603
                     /locus_tag="KPB88_003843"
     CDS             1548..>2603
                     /locus_tag="KPB88_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBG0056070.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQ"
BASE COUNT         1168 a          246 c          432 g          757 t
ORIGIN      
        1 taaattagag tatttatcaa ttcatagtag ataaaattga aattaaatat aacaacaaaa
       61 aatgaaaatt acaattaatt taatgttaaa gactcaatta tttggatgtg gaatggatag
      121 tacaagtagt atataaaaag tattgactgg acaaataact atattttatt ttaaatatat
      181 aatataatgt aataaaatat atcaaatgta gaaggtgata acctatgaaa aaaataacaa
      241 taaatgatat tgctaacttg gcaggagtat ctaaaagtac tgtatcaaga tatctaaata
      301 ataaagatat cagtgattct actaaagaga aaataaagac aataatagat gaatatggat
      361 atgagccgaa tgcatttgct caaagtttga gagctaaaaa aacatacttt attggtatta
      421 taacaccgtg tttagattct tttgtaaaat ctaaaattat gatggcaata gatgaagaac
      481 tcaaagaatt aaaatataca tcactaatta ttaatactaa tagaaaaata aggtcagaaa
      541 tagatagtat atcaaaatta gctagtctaa aagttgatgg aataatacta attggtaccg
      601 aaataactaa agaacataag aatgaaattg aaaagttaga tataccgata gttgtagttg
      661 gacaaaaagt tgatggtatc aatagtatag tgaatgatga ttatggagca ggatataaaa
      721 tgggtcaata tattgctaat aaaggttaca aaaacattgt atatttaggt gttgatgaaa
      781 gtgatatatc agttggttta aatagaaaaa atggagtact taatgggtta aaagataaag
      841 ggtatgatgc aaaagtattt tatactgact ttgaccaaga aacgtctata caaagaagtg
      901 gagaaatgct agaaagtgaa aatccagata taataatatg tgcaactgat aatatagcaa
      961 tagcaacaat gaaagaaatc aataagagag gtaaaagtat accacaagat atatctgtag
     1021 ctggatttgg gggatatgat gtattgtcta ttataagccc taaacttaca acaataaagt
     1081 ttgaaaataa aaatgcaggt aaagtagcag caaatacgat agtaaactta atacaagaaa
     1141 gaaaagaacc actattaaag gagattaaat ttgaattaat agaaggtgaa agtacaataa
     1201 ataaaaatta agatacacat tacatatatt agtaatgtgt atttttttac taaaaaaact
     1261 ataattctaa aaattaggaa aacttttttg aaagaaatat tgcatctgaa ataaatttat
     1321 gctaatcttt aattaaaccg gtttcgaaac cggtttaatt aaaccgatag aatagcaaaa
     1381 tgagtgcaaa aataactgat tgtaacaaag tatcaaagta attattagag tacataggaa
     1441 ataaataaaa tataaaaggt gtagcacatt gtgtaacaag attaagaatt gttttagata
     1501 gtaataataa agcaaatata aaattataaa agaaaaggaa taaaaatatg tacaaagagc
     1561 ctaaatatag aaccatatta gaatcgacaa aagaagaatt aaataaatta agaaatatat
     1621 ctttaaatga taaatataaa ccactatttc atatacatcc acaacatggc ctacttaatg
     1681 acccaaatgg attagcatat tataatggta agtatcatgt attttatcaa tggtatccat
     1741 atgatgcaac tcatggaatg aagcattggg cttatgtaag ttctgatgat tttgtaaact
     1801 ggaatagaga agatgtagca ttaataccga tcgaaagtta tgaatctcat ggagcatatt
     1861 caggtaatgc gatagaggta gatggaaaat tgcatatgta ttatacaggg aatataaaat
     1921 atagtgcaga agatagatat gcatatcaaa acttagctat aatgaacaag gatggtaaaa
     1981 taactaaata tgaaaataat ccaatagtaa gtgaaatacc aaaaggatat acaggacatg
     2041 taagagaccc aaaagtattt aaaagaaagg ataaatactt tatgttactt ggagcacaaa
     2101 caagtgataa aaaaggagtt ataatagtat atgaatctaa aaactctata gattggaatt
     2161 ttaaaggaga attaaatgta aaaaatatag atgaagattt tggttacatg tgggaatgtc
     2221 cagattatat taatatagat gaaaaagata tattaatatt ttcaccacaa ggagtagaac
     2281 ctaaaggatt tgattatcaa aatatatata atgtagtata tgctattggt aatatggatt
     2341 tagataattt aacatttgaa atagatacaa tgaaagaact ggaaaaagga tttgatttct
     2401 atgctccaca aacatttata aaggactcac aaataatact atttgcatgg gctggaatgg
     2461 gagaagtttt ataccctaca gataaaaaca aatgggcaca ttgtctaact gttccaagaa
     2521 aattaaatat aaaaaataat aagctattgc aaatgccagt agatgaatta attaaattaa
     2581 gatatgatga aacaagtggg caa
//
LOCUS       DAELJO010000337         2592 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.339, whole genome shotgun sequence.
ACCESSION   DAELJO010000337 DAELJO010000000
VERSION     DAELJO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.339"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1017
                     /locus_tag="KPB88_003844"
     CDS             <1..1017
                     /locus_tag="KPB88_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG0056071.1"
                     /translation="NYKKVKSTIEKLETRGKMPKWYLELINKVKNILENMLYNRNLSD
                     EQIFYLNLFLDIQNLFLGIYELKSESVIFENTLDDNKIIAKMDTYIIIDYINASFEYL
                     GYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKIETPKPIVGENYVTVRDAIGD
                     LAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIYNHVCTDTRNIALKRFEYINQ
                     GNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAVSAR
                     EAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVAEKILNLFNDEPNEYLRDVFE
                     ALDG"
     gene            complement(2017..2589)
                     /locus_tag="KPB88_003845"
     CDS             complement(2017..2589)
                     /locus_tag="KPB88_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG0056072.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
BASE COUNT          985 a          307 c          348 g          952 t
ORIGIN      
        1 aattataaga aagtaaaatc taccatagaa aaattggaaa caaggggaaa aatgccaaaa
       61 tggtatttag aattaataaa taaagtaaaa aatatattag aaaatatgtt atataataga
      121 aatttatcag atgaacaaat attttatttg aacttattct tggatattca aaatttattt
      181 ttaggaatat atgagttaaa atctgaatct gtaattttcg aaaatacact tgatgataat
      241 aaaattatag ctaaaatgga tacttacatt ataatagatt atataaatgc atcctttgag
      301 tatctaggat atgtattaaa tggaagtgca ctaaattcaa ttgattatgg agtacctcaa
      361 aatagagaaa gatttgtatt gataggagca aaaaaagact tcttaaaaaa taaaaagata
      421 gaaactccta agcctatagt tggagaaaat tatgttacag taagagatgc aataggcgat
      481 ttagctgaat atgaggcttc taaaggtagt atggattatt gttttgaaaa aaatagtaat
      541 aatattgaaa aagattttta tagaaagata atattggata gcaataaaat atacaatcat
      601 gtatgtacag atacaagaaa tatcgcacta aaaagatttg aatatataaa tcaaggaaat
      661 aattttcatt ctttgccaga tgagttaaaa ggaacttatg ctgatccaga aagaactcaa
      721 aatactattt ataaaagact agtatatgat aagccatcag atactgttgt aaatgtaaga
      781 aaatctatgt ggatacatcc tattaaaaac agagctgtta gcgcaagaga ggcggctaga
      841 ttgcagtcat tccctgatag ttataagttt ttaggtacaa aagattctgt atatcaacag
      901 ataggaaatg cagtaccacc tttattaggt cgagtagtag cagaaaagat attaaattta
      961 tttaatgatg aaccaaatga gtatcttaga gatgtgtttg aagcactaga tggatagttc
     1021 aaataaaata gtgggtatta ttcttgtgat tcttatccaa cattaatcca cgaaataagg
     1081 actgtcgaat gagtaatttt aaattgctgt gcgacagttc tttttttact tgcttttcat
     1141 taatttatat ttatgcaaaa tttgagcttg aaaacggtca taagaaataa aggtataaaa
     1201 atacaatttt aaaatagaat aatttacaaa gtgtaagatt aaaaaatatt tttgagagga
     1261 tgaatataca tactacattt attaatgatt atcttatttg tgaaacttag aaatactata
     1321 ggctaacact acaggtataa actctgaaag aatattttga ttgcctttga ttagaattct
     1381 tttaaagttc atactatgtt ttatttgggt gaaagctcct tatgcctgaa tacttctatt
     1441 cattctaagt tcacatcctt gtgtaccagt gattctttct aaattatctt aatgccaaaa
     1501 tcttgttcgc atttcaaaat aaagtcaggt agctacttca acattttagt tatatttctt
     1561 caatagtttg tctccaaaca aaggtacatc tattagaaac agatttaatt ttatttatat
     1621 caataaataa tatatacttt gaaacttcat taatatcacc aagataattt ataagttaag
     1681 ctattatata ctatcttcac caactacatc ctccatagtt ttacactttc ttaattcagc
     1741 ttggtaatct aaatctgtaa taattatttc tctttgattt ttcatttctt tgcctacaaa
     1801 agtttaatat aaatattatt ccatacttac caattttacg ttcaaaaaag actgtagata
     1861 agtctgcatt tacacaaacc tacttatagg tctctttttt gaaattaatt tagcatgaac
     1921 tatagtaaga gcatctttaa attcatagtt acaagtatac aaagttataa ttttatctga
     1981 aatatctacc tttatatcag atttgtgtac tgatttttag gttatataat tcaaagaata
     2041 tttaaactca tcattattat taaagtgtat acttaaataa tctctgttaa ttaagtcagc
     2101 agtatatact gaaaaaattt cataagtata agtttgtact ttataattta tttctactaa
     2161 gttattttct ttgaaaaata cttcatcagt atagttttct agttgaccaa acatagtttt
     2221 atttttcata taatgaccat agactattaa actcttatca ttttcaaaat tatttctata
     2281 atccatgaaa atactaccta aaggaagata atttttataa aaatcatgag ttaggtagaa
     2341 atcattgtta tatccttgta ctacaggata gtttatattt acgttgttta ttttaagcca
     2401 gaatctataa tcattatttt tatcataaag ttcttgatgt ttgctattat tgatagtgtt
     2461 ttctctgagt tccgtatata cttcatcagc ttttttatat tcagataatt ttatgtaaat
     2521 cgaaaggcta gagaaagtta gcgcaataat gcaaataata tttattaata tttttactag
     2581 tttttcaatt aa
//
LOCUS       DAELJO010000338         2592 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.340, whole genome shotgun sequence.
ACCESSION   DAELJO010000338 DAELJO010000000
VERSION     DAELJO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.340"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            83..556
                     /locus_tag="KPB88_003846"
     CDS             83..556
                     /locus_tag="KPB88_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056073.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGIMTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(925..1482)
                     /locus_tag="KPB88_003847"
     CDS             complement(925..1482)
                     /locus_tag="KPB88_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0056074.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            1740..2438
                     /locus_tag="KPB88_003848"
     CDS             1740..2438
                     /locus_tag="KPB88_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0056075.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQRGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLIECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          973 a          326 c          310 g          983 t
ORIGIN      
        1 atttataaaa caaattgtat tgaattgaat ttatttaaga tatataatta taattaaaat
       61 aataatacag gtgttgttta taatgttcaa agaatactta ataagagaat taaaagaaga
      121 tgatattaaa aatgcattag acttaatctg gaacgtattt gaagaatttg aatctccaga
      181 ttattgtgat gaaggaatta tgacatttca tcaatttata gaatataact ctattattga
      241 aaaaatcaaa gaaaaaactc tcttcttttg gggttgtttt gactctaaaa aacttattgg
      301 agtcttagct attagaaatt taaatcatat atgtatgtta tttgtagata aaaactatca
      361 taaacatggt gttgcaaaaa aattaatgac tacaggcata caatactgta tttgtaataa
      421 atttaattta actgaaataa ctgtaaattc atcaccttat gctgttgaag tttatcataa
      481 attaggattt aaagatactg ctgttgagca aattgttgac ggtatacgtt ttacacctat
      541 gaaattgtta atataataaa ttaaataaaa agcaaagtct cttaaatatt ttatagttac
      601 tttgctttta aaccaagatt taaaataata ctatcttttt tgttttttaa taatatactt
      661 taaaatcctt actaatatga actataattt attttttgat atcctttttg attaacatat
      721 aaattaattc aattattaaa catgaactat gttgtttctc tttatatctc ttctttgtaa
      781 acaatattgg gcatttgaaa ataaatattc ttaaaatata ctgttctcct gttgattttc
      841 ctttggtaca aatgataaaa atttaattta catgccccat aatgaagtgt tcaataaata
      901 agttaaaata atttatttac ctccttaatc atatttttta tgaataccta ataacacagt
      961 atcaatagaa tcgactaaac cttttatagc ttcttcttta ctcaaaaatg atttcccaag
     1021 ttgcaaagtc gaataaccat gaagtacact cattattaag tttaatactt cacctgtatt
     1081 ctttgtattt agattacaag aaagaataag tttttctaat aaggatttat attcatcaaa
     1141 gatttctaat gtttcattat ttccatgcca agtagcccac tgaattgttt cataaacacc
     1201 aggattagaa attataaaat taaagtatgc tatacttata cactttatag ctgtatctcc
     1261 aaaatttcca atagtcgcct ttatcatttg actattcatc tctctcattc ctttatgagc
     1321 tacttccagt aataaatcat ctagactttc tatatgattg tacaaagatg gtgtacgtat
     1381 tcctagcttt tctgcaataa cttttaaaga aactttgtta agcccatctt tattaactat
     1441 atctgatgta gcttgtataa ctatttctct actaacacgc atgattttcc ctctttcatt
     1501 taaaattaat tactttatta gtttaactaa tctaattagt ttcgtcaatt attgtaattt
     1561 ataaaattct ttatactatt ataaaatctt gacagtttaa ttataattta atataatact
     1621 aattgaatta gttttaatac taacataatt agtattatat taaattctat attacaacat
     1681 tattttatag tttgattaat ttaacaatca ctaatatatc aaactgaaag gagattttta
     1741 tggaaaaaaa atatttcgaa attcatggag ttccttcaat cctttgggga aatccttcaa
     1801 gtaaattata tatttacata catggacaac gtggcaataa agaggaggct gaagcttttg
     1861 ctaatattgc agttccacat gagtgtcaag ttttaagtgt tgatttgcct gaacatggag
     1921 aacgtaaaag agaaaaaaat tcatttaacc catggcatgt tgttccagaa ttagtatcta
     1981 ttatggaata tgcaaagtgc cactggaatc aaatttcgtt atatgcaaat agcattggag
     2041 catggtttag tatgttaagc tttgaaaaag agaacttaat agagtgtatt tttgtatctc
     2101 caattctaga tatgcaacag ctcatttcaa atatgatgct ttgggcaaat gtatcagaag
     2161 aacagcttaa acatgaacta attatcccaa cttcatttgg gcatactctt tcatgggaat
     2221 atctacaata tgtaaaacaa catcctatca ctcaatggaa tgtaccaaca aaaatattat
     2281 atggtaaaca tgatgaactt actgaaatcg gtgttgtaga gaaatttgta gaaaagttta
     2341 attgcaacct tactattata gaaaatggca aacattggtt taacacacca caagaattaa
     2401 attcattata tgaatggtta aatactttag ttaaataaaa aataattata ttattaatat
     2461 gattaaatta tttataaatg ttatttaatt aattttattt tagcattatt aaaataacta
     2521 cataaagaat aaattagtta ttattaaaga aattaaaagt atatagagta ttaataattt
     2581 tttaataatt ag
//
LOCUS       DAELJO010000339         2563 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.341, whole genome shotgun sequence.
ACCESSION   DAELJO010000339 DAELJO010000000
VERSION     DAELJO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.341"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            486..1307
                     /locus_tag="KPB88_003849"
     CDS             486..1307
                     /locus_tag="KPB88_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0056076.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKDNEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(1617..2396)
                     /locus_tag="KPB88_003850"
     CDS             complement(1617..2396)
                     /locus_tag="KPB88_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0056077.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          935 a          371 c          304 g          953 t
ORIGIN      
        1 atatactatc cttatactct agttaaatat attttaatca ctaatataaa ttagtttttt
       61 taacaaaaca ataaaagagt aaagtacaaa taattccttt gtgctctact ctttttaact
      121 ttctttagac aataatacaa tgataaatgt aaaaaattat atattccatt ctatagaaat
      181 atgctcacca ataacatgta tgttgttaat caatatatta tatcaaatta tatcaaaatt
      241 acataaacct tataggacaa tcatatctta ttacaagaaa aatgtcttat ccatattgaa
      301 taagtcattc agttcatata caaagtcaat ttcattgaga aacatttaat atatgtttat
      361 aaaaatttca tactattctt tcatatttgc atgtgatata atttattaac tcataatttt
      421 gttaatcagt tgtgcttgta tattatttat ataatctaat attttaagaa ggtggtgtaa
      481 taactttgaa attaaaaatt gttggtagtg gcggtatgtc aattattcca agttcatttt
      541 gcaaatgcaa aatatgtgaa gaagccagac aaaaaggcgg aagatatgaa cgattagggc
      601 caagtttata tattgatgat ataaaaatgc tgatagacac acctgaagac attgcagtag
      661 cttgtaacag acaaaaaata tcggaagtaa aacatatttc aatttctcac catgacccag
      721 accatgttaa gggtattaga attgtcgaaa aaataggatg tgactttatc acaggagaaa
      781 ataatccaat tggcttttat gcgcttcctg aagtaattga agatataaac cgaacgaatc
      841 tcgattgtct taaatattat ggtgatgtta taaattgcat ttctattaat gaaacttcgc
      901 atgtaaagat aaataatttt gatattgatt tgattaacaa taatccagac aggaatataa
      961 ccttctatgt tatcaaagat aatgaaaaaa aagtcatata tgcctgctgt aatcccaagc
     1021 catttactca tagtgatatg tactttgatg ctgatgtttt aataataagt ctagtttctg
     1081 atgatggtat attaaatgat ggtacaaaac ttgaagacac accatttaaa gatgaaatat
     1141 ttactttaga tgagattgtt gaaattaaaa actattatag aattaaaaag attattgtta
     1201 cacatattga cgaaatgtgg ggaaaatcat atggttatta cagtgaatta gaaaagaaac
     1261 ttgataacat ttattttgca tatgatggta tggaaattat agtctaaatc attaagtaat
     1321 aagcataatg caaaataaga aaacttcaat ttaacagtat agtcaaacta tgtgatatca
     1381 acatatctca gtttttacaa aatatcatag tatttatctt tataaaattt caagtcctac
     1441 atattttaat aattcaatat ctaaataaag taaaatcaaa aatatatttt atatacatta
     1501 tagacggctt atcggcacta gataagccgt tttgtcacaa ttattatttg ctcaacaaaa
     1561 cttctcataa tatataacag attgaatctc caactgatac ttaattaccg aaactcttat
     1621 tccttaatat gttgttgcat taaccaaacc ttcatcatca ttttttgtgg caatagtttt
     1681 gctaataaat gactggattt tgtatttata ccatacacag aaatatcctt atttttcttt
     1741 gcatccttca atgctttttt ggcaacaatg tctggagaca ctatgcctgg gaaatttttt
     1801 gtcccttttt cagctacaac tatacctctc ttaaatagat ttgtactcat ccaacctgga
     1861 catacagcaa ttgcatttat ccctttactc ttaagttcaa catttaatgc tcttgtataa
     1921 tttcttataa acgctttagt tgaactataa atattttgat atggcaatgg ctggaatgat
     1981 gcttgtgaag aaatattcat gatatggctt cccttttgca tatatggaat acatataagt
     2041 cccaatgcta caacagcact aatatttaaa tcaatcatat taatagattc atctatactt
     2101 aaatcattgt atgaacagaa ttttgcaaaa ccagcattgt ttataaggta ttttatacat
     2161 acattttctt tacttaaaaa tgtaccaatt tcttttactt cttctatttt tgataaatct
     2221 tttgaaaaaa tttttacctt acttccaaat tcatctacta attgatttaa tctcgccttg
     2281 tttcttgcaa ctgcccatat ttctgttatt tcttcttcat ttattaataa ctttagaaac
     2341 tctttaccaa aaccactatt agcacccgtt ataattgcaa tcttcttata actcataatt
     2401 attctccttt accataagtt tatacgctca aactgtaaac ttatttttta caaataaaac
     2461 accctctatt cagatgttat attcatatta tttgagatat tatatttata tcattttcaa
     2521 taattgttat atagcataaa aaatagtgtt aatattatag aat
//
LOCUS       DAELJO010000340         2562 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.342, whole genome shotgun sequence.
ACCESSION   DAELJO010000340 DAELJO010000000
VERSION     DAELJO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.342"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            48..224
                     /locus_tag="KPB88_003851"
     CDS             48..224
                     /locus_tag="KPB88_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056078.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            450..650
                     /locus_tag="KPB88_003852"
     CDS             450..650
                     /locus_tag="KPB88_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0056079.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   1013..1243
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1079..1109
                     /rpt_unit_seq="actttacataccacatagttgatataaaacc"
     gene            1469..1651
                     /locus_tag="KPB88_003853"
     CDS             1469..1651
                     /locus_tag="KPB88_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG0056080.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            1722..>2562
                     /locus_tag="KPB88_003854"
     CDS             1722..>2562
                     /locus_tag="KPB88_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056081.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHL"
BASE COUNT         1008 a          291 c          341 g          922 t
ORIGIN      
        1 tgttcttttt gtttttatga aagtttttta gggggataat aatttatatg tttggaaata
       61 aaatggataa atgtacacat gtattgactg cttatattag cagttcgtat gattattgta
      121 attttttaga tacacagtta gatgatttta tattagagta cggagaaaat gtagtagagt
      181 cttgtttaca ccaagtaatg gtattggtaa gtaagtataa ttagatagac attgatttct
      241 agaaaaccct ttttagtttt atctacattt atattaatat atagcaatgt aaaacgttta
      301 aatatatttt taatattaag aataattaaa atgttaacta aaaaatataa ttaagtagac
      361 atttctatta atctatgata caatagtatt tgtggtaata cacaattaaa tcaagtatat
      421 cttgatatag gttaatagga gtatttttaa tgaataatgg aatagtgaaa tggtttaata
      481 atgaaaaagg attcggtttt atatcaatgg aaggtagaga tgatgtgttt gctcatttct
      541 cagctataca aacttcagga tttaaatcat tagaagaagg tcaacaagta agctttgata
      601 tagttaaagg tgctagaggt cctcaagcag aaaatataac tatattataa tatatagatt
      661 tttcgggaaa gactcttttt agagtctttt tttattgtta atatatgtaa gataatcatt
      721 ggtgaagtaa ataaggtata ttgagtaatg ttcttgatag tttatatgct aatgaatttc
      781 aaaaaaataa gcactcctat aaaaagagtg ctcttaatgt aaatataaac cttcattggt
      841 ccagcctaat accccgtatt ctccaagttc taactctttt ttatatacat tatcactttc
      901 agccatatct aacacaatca tttcttcttt agaatcaatt tgtaaaatat gaggactgtg
      961 tgttgtgaca attatttgtg tttttgggag aattgctttt aatgtaataa aaattttaca
     1021 taccacatag ttgatataaa actattaagt tttctatgtt tatattcata ttcaaacaac
     1081 tttacatacc acatagttga tataaaacct tagtgtataa acatccttta aggtcaatag
     1141 gctcttgtct ttacatacca catagttgat ataaaacatg tagttgttac tttccagcta
     1201 cactcatgct ccactttaca taccacatag ttgatataaa accccaaaat aaacttagca
     1261 tctccaatgt ttacacatat acaatgctct taaaattgca gtgaacgcgc aatagtgcaa
     1321 ttgataatac ttattatata ttctcaatgc cttatatttc aactgctaaa cctcaatttg
     1381 tcacaaatat cgctcactgc aaaatttcta tatttttatt atatcataac atataattaa
     1441 tattttcaat taatttttac atttaattat gtttaaattc tataataatg caactttaat
     1501 gtttctttct ggaactaata taaaaactat ttcagaaaga ttaggtcatg cagatataaa
     1561 aataactatg aatagctact ctcagttttt agaagagatg gacagagaag catttgaaaa
     1621 tttgagcaaa atactattta ttgtatttta aagaagatga cttattcaat atttgatgct
     1681 agaatataat taataaaaat ttaattatag gtgattgtat tatgaatatt attaaaagca
     1741 taactttact cttaacatta ttaattgtga tattaatgac aggctgttcc ccaagagaaa
     1801 gtgctagtga agttgcaaaa aaatatattg ataagatgaa ctctggtgac tatgaaacag
     1861 catatagttt attaagtaaa gatagtaaaa aaaaaattag tatagaaaat cttgaagaat
     1921 ttcaaaatat tttagttagt acaaaaagaa taattagtta taaaataggt aaagaaaaaa
     1981 tcataaaaaa atataaacat gatggaaaag aatataaaaa tgttgttaaa ttagaagaaa
     2041 cttttaatat aaaaaacctt ctttataaaa aagatgattc tctaaaagta aatagatatt
     2101 ttgtagttga aaataacaaa tacaagctat tattgtatga aagtttcaaa aaagaatttg
     2161 gtattaatag tatggacctt gctcaactta agcttaatga ttttggacta aaatacttta
     2221 aagaagttga ccttatacta aaaaaagcta ttgctattaa tcctactgat tcaaagcttt
     2281 attatgccca agcatgtaat tatatgaatt ttgatggatt tcctggttat tcagatacaa
     2341 catataatgc tttagattca attaataaat ctttaaagta tttagataaa aatgattctg
     2401 actacaaaaa aaatgcaagt aatatatata acttaaaagg agttattctt atgtttcaca
     2461 aaagatttga tgaagcacaa caatatttaa ataaagcctt atactttaac aaagataatg
     2521 aagacccaaa atataatcta aatacaataa aaaaacatct aa
//
LOCUS       DAELJO010000341         2521 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.343, whole genome shotgun sequence.
ACCESSION   DAELJO010000341 DAELJO010000000
VERSION     DAELJO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.343"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(582..1133)
                     /locus_tag="KPB88_003855"
     CDS             complement(582..1133)
                     /locus_tag="KPB88_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056082.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKILKAVIINYYPV"
     gene            1324..2286
                     /locus_tag="KPB88_003856"
     CDS             1324..2286
                     /locus_tag="KPB88_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG0056083.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          966 a          271 c          413 g          871 t
ORIGIN      
        1 taccattatg ttttaatata ctattcaatt atttaacttc ttaaattaca tttatatata
       61 ggagtcttat gataaaagta agcttatgtg ttatttgtag taaatataaa gtgcatatag
      121 tgataaaaca aaagtttcat ataaatgaag aaaaaatata aaataattta tttaactact
      181 taaaaataaa ctaaaaggga taataaaata gcttttaggt tatggtaata aaggtattta
      241 aaaagagtgc ataagcactc caattgtgag aaatacatac acaaatcatt attacaacaa
      301 tgaaaaatgt acatataaga gacgttttgt atttgtttta agtgataata ttttaatact
      361 aactaaataa ttatcacaat agtaaaaaag aaaaaggagt attttgtact ccatttgtca
      421 aaaatataaa acttttatat aagcttatta taacataatt gataaaagtg taaagatata
      481 taatgaattt ataaagaaaa ataaaataaa aaagataaat cctaatcaaa ggatttatct
      541 ttttgtatag aaaataatta ttctattatt aaatggcatg gctaaactgg ataataattt
      601 attattactg ctttcaatat tttttctcct tgattatggt atgtatgaat tgattcagct
      661 gaaaaacaaa tagagtcatt ttctctaagt tgataaatag agtcatttac ttctaatttt
      721 aattgacctt ctataatcat aatatattcc tgtgattttt cagaatgacc aacagatgta
      781 taactatgac cttcttctaa ttccatttga aataattcga agtttctagt tggtgtattg
      841 ggataataac aataaagacg ataatcttta ttttctgaaa cttgtacatc tatatcagtt
      901 tttgatacaa taaaagtttc attttgaggt tgttccagta ttgcagtata tggaacattt
      961 agtccacttg caattttcca aattgtgttt actgttgggt ttgagtctcc cttttcaatt
     1021 tgcgaaagca ttactttact tacgccagat aattcagcaa gttgacctag acttaagtta
     1081 cgttcattac gtaatctatt taaattttta gcaataacag tattaatact catagtagct
     1141 ccttaaaatg taaattattg acaaatataa tttacgaatg tataatttat ttaacattat
     1201 ttataattta caaatgtata atataatgaa cgttaaatac actgtacatc cgtaatataa
     1261 attatactgc ataaagagtt gtttttcaac ggatgatagt tgagagatta agggggaatt
     1321 gtaatgcgtg tacttaataa tgttacagag gctataggca atactcatat gttacattta
     1381 tccaaaatag cagatgaatt tggagttcaa ggtaatattt atgcaaagat ggaatactta
     1441 aatccgggat ttagtaaaaa ggaccgtgta gcattacaaa tgattaccga agcagaaaat
     1501 gcaggtttat tagttccagg tcaagcagtt gtggaattaa caagtggtaa tactggaaca
     1561 ggactagcaa tagcttgtgc atgtaaagga tataaattta tagcgtgtat gtcaaaagga
     1621 aattctatgg aaagagcaag aatgatgaaa gctttaggtg ctgaagtagt tcttgtagac
     1681 caaatgccta attcagttca tggacaggta tctggtgaag atttggcact tgtagaaatt
     1741 gaggctcaaa aaatagcaaa agaaagaaat gcatttagag cagaccaatt taatttagaa
     1801 gcttgtaatc atgcacatga aaagtataca gcagaagaaa tgtgggaaca attggatgga
     1861 aatattgatg tatttgtaga ttttcttgga agtggtagta cttttgcagg atgttcaaaa
     1921 acacttaaaa aacataattc ttctatcaaa tgttatgttg ttgaacctga aacagcagca
     1981 atttattcag gtaaagaaat aactgaccaa gggcataaaa tacaaggtgg aggatattct
     2041 atggatttgc cattagtaga caagaaactt attgatggtt atattcaagt tacagacgag
     2101 gaggcaactg atgtagcaag aaaattagct aaattagaag gtgtatttgc aggattttct
     2161 tcaggggcaa atgtatgtgc agcaattaag ttgttaaaga gcagtgaaaa aggaaaaaat
     2221 atagttttaa ctcttaatga tagtggttta aaatatttaa gtactgattt atatgaagaa
     2281 ttgtaataat agcacaatag ttatgcaatg ttttttaaat taagtatata gatttaaatt
     2341 ataaaaagta tctcaatgtg atttttgaaa gttataaagg agatactttt ttattttgta
     2401 aatatggagt atcctactta taagtattaa gctatataga tattgatgat tagctatgca
     2461 ataaaaaaga gtatttgttt tgcatttata catcataact tggaataata catattaatg
     2521 t
//
LOCUS       DAELJO010000342         2509 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.344, whole genome shotgun sequence.
ACCESSION   DAELJO010000342 DAELJO010000000
VERSION     DAELJO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.344"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(136..1509)
                     /locus_tag="KPB88_003857"
     CDS             complement(136..1509)
                     /locus_tag="KPB88_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0056084.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHTIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(1545..2480)
                     /locus_tag="KPB88_003858"
     CDS             complement(1545..2480)
                     /locus_tag="KPB88_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0056085.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRAECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
BASE COUNT          894 a          469 c          267 g          879 t
ORIGIN      
        1 taattcaaaa tattgtatac ttatcgagta ctctatgctt caaaacttag aattaataaa
       61 tatcaaacca gttatgtttt acaaacaata tttttaaact tatcagttac tagacttatt
      121 aaatccataa atccactatt ctacttttct taattctttc tttaatataa aatttaataa
      181 aaaacttatt gtacctaaga ccaccataac ataacctaat atctgaaatt gttctgatgt
      241 tatatttact tcaaatacca ttccaagtaa aacagttgct aaaatagaac ctaaataacg
      301 actagtttga aacagaccag aagctgtccc tatactgtca cgaggacttt cttcaaacat
      361 agaggattgc agggctacat taccaactcc ataactaata cctgctaatg ataatataat
      421 accctttcct attacaggta tatggataaa aactactgaa agaagtactg cactaataaa
      481 cataaagatt gatgatgtaa caagaggaaa tctagttcca gatttatcta tccattttcc
      541 agttaatgga gatataaata tacctatacc agacataaat agcataaata atccagtcga
      601 ctttgcacta taatgtaagg catcttgaaa ataactagga agaccaaaaa acaaaatata
      661 attaaagaaa tttaatataa taaattgcaa gtaaactttt gacaatttag gattacttac
      721 aaaaagctta atatctataa atggcaattt tgttctcaat tcatgccaaa taaaaagaca
      781 catcagtatt ataccaaata caagttgttc taagttaaaa gattccttca tagatagcaa
      841 aaatagtaat ataaaaatca ttgcaattga aaatagaagt atgccaataa tatctaatct
      901 tgacaaaaca ttttttaaag tttcttttat agatttcttt tcttttactt cttttgggaa
      961 taaaaaatac cctaagcata tagaaaccaa aacaacaggt atatttacaa gaaatatagt
     1021 atgccatcct cctaagtcca acaataatcc tccaagtgtc ggacctaatc ctgctgtagt
     1081 tgaagcaaaa atggttagaa ctgctaatgc agaagattgt ctttctttta tattattttg
     1141 tataagagca actccagatg gatatagggt acttgttcca attgcttgaa ttactcttat
     1201 aaaaatcaat ataaagaatg attgaacaag tggtactatg gatgctgata aaattacaag
     1261 catcaaacca ctcaaaaata agacctttct acctattaaa tctcctatct tacctgaaac
     1321 tggttgtgca atagcactag ctaggtaaaa tgctgaaaca atccaagata cagttgtaaa
     1381 actaatatga aaatcatttt gtatactatg taaagcaagt gaaatcatag aagaattaag
     1441 aggattcagc atagttcctg aacctattgc tatcaaaaaa agcgttcttc tagcatttgt
     1501 tttacccata tatcatcacg cctccaataa gcagttcatt tacattataa aaaagctgct
     1561 cctataattc ctgcgtctga acctaaagtt gcacatgtaa ttgtagctat atcttctgtt
     1621 ttgtataata caaattttct taccaaatct tctattccat caagtattat atcgctagat
     1681 ttagaaatgc ctcctcctat agatataatt tctgggtcta aagaatttat tgtgtttgca
     1741 aaagtttttg ctaaatactc tttaaatcta tttattactt taattgccac taaatcattt
     1801 tctctatatg catcaaacac catctttgca tttacatttt ctaaatttcc ctctgccaaa
     1861 tctaaaatcc tactcttttc tccttcttct atcagttttt gagaatactt tataattgca
     1921 gttgctgaac aaaaagtttc tacacaacca ttatttccac aattacaatt aaaatagttt
     1981 tctcctataa tttgatgacc tatttctgaa cccaatccat gtgctccaga aaagactttt
     2041 tgattaatta ctattcctcc acctacacca gttcctaaag tatataatac tccaacatta
     2101 gcacctttca tacttccaaa ctttgcttct ccaagtgctg ctactgttgc atcattttct
     2161 ccatgaattt caacatctgg aaatcttctt tttaactctt tattaaaagc ttttttattc
     2221 cattttaaat ttacacaagt tactaatcct tctttattta taaaacctgg aacaccaaat
     2281 ccaatagatt ttatgtcact catagtaagt ttattatctt ccaacaactt ataaatcatt
     2341 atttttatgt cattaagtat tccttcaaag cctttttcaa ttactgtttt acactctgcc
     2401 ctaaaaatta ttttcccata gttatcaact actcctgctt gtatacctgt tcctcctatg
     2461 tctacaccta tataatacat ctcttttcac ctttctcttt tgaatcatt
//
LOCUS       DAELJO010000343         2486 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.345, whole genome shotgun sequence.
ACCESSION   DAELJO010000343 DAELJO010000000
VERSION     DAELJO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.345"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1051
                     /locus_tag="KPB88_003859"
     CDS             <1..1051
                     /locus_tag="KPB88_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0056086.1"
                     /translation="FLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGVS
                     ISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRAG
                     AEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVNY
                     NEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDV
                     VKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDI
                     YGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAIY
                     WKKNGFISQVSDYLF"
     gene            1373..1957
                     /locus_tag="KPB88_003860"
     CDS             1373..1957
                     /locus_tag="KPB88_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0056087.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          985 a          230 c          393 g          878 t
ORIGIN      
        1 atttttgaag aatgtattta taaatagatg tattgcattt gaagaatatt ttagattgat
       61 tttgtcaata ttcattttta tattggacat tggatttatc tcagcaactt attatcaata
      121 taaaggagta agtatttcta aaaataatat taaaaaagag aaaaaatcta tatcaaaaat
      181 tgtttttaat ttagtattaa tattattaat ttcttatata gagtcagttg cattttcttc
      241 acatataaat ggtatgttta atacgtcatt ttcattttat acaacagcaa ttgctcatag
      301 agcaggagct gaaatggctc cagagaatac tttggcagca atagaagaag cttcacatgc
      361 taaagctgat tatgcagaaa tagatgttca agaaactaaa gatggagaac ttattgtact
      421 tcatgattca aactttaaga gaactacagg agtagataaa aatgtttggg atgtaaatta
      481 taatgaagta aagacatatg atgcgggaag cttttattat tatggaaaag gttattatga
      541 tgaaaagttt gttggggaaa agataccaac actcaaagaa atgataaaat attctaaagg
      601 taaggtgaaa ttattaatag aaataaaact taatggacat gaaaagggag acgtagtaaa
      661 aaaagttgtt cagttaatta aagaaaataa ttttgaaaat caatgtatag ttgcatctat
      721 ggataaaact atattacaaa aagtaaaaaa actggatgag aatttactga catgctattt
      781 tatggcaatt gcatatggtg atttttcaaa gttaaattat gttgatatat atggaattga
      841 atcaactttt gttaataaaa cagtagtaga aaagattcat aaaatgaaca aaaaaatctt
      901 cgtttggact gttaatagtg acagtttaat tgagaaaatg cttgacctaa atgtagatag
      961 tattattaca gataatccat accttataga gagtgcaatt tattggaaga aaaatggatt
     1021 tattagtcaa gtgtcagatt atttatttta aatattctat aattgtatta tccagtaaaa
     1081 gagatattat taaaatcaat aatatctctt tttaacataa ataaggttgt caaattaagg
     1141 ttattcttat aaaaattgtg cttatcagct ataaacaagg aataaaagtg aaaaaattgc
     1201 tctgagatta ttacagttaa aagagtttta agatgacaga gagatagatt gtcaactgta
     1261 tatttaataa atcttgttat ttattaaatt ttatataagt aaaatatata aattaataaa
     1321 tcttatgcta gaatatatat gaaaaacaat ataaatttgg aggattaaaa acatgacaga
     1381 gcgagaaaaa atgttggcag gagaacttta cgattgtggt gatattgagt tgttaactca
     1441 atggcataaa gcaaaagacc tagtaagaga ttataaccaa acagattcct cagatttgga
     1501 aaaaaaagaa aaaatattaa atgagttact tggtggtaag ggtaaaaact tatggattac
     1561 tgcaccgtat tatgtagatt atggaaataa tatttatttt ggaaataatt gtgaagtcaa
     1621 tatgaattgt acgtttttag atgataataa aattatcatt ggggataatg cactaatcgc
     1681 accaaacgta caaatatata ctgcatttca tccaactaat gctggtgata gatttggtaa
     1741 tgccaaagaa gatggttcat ttgaattttg taaaactcaa acagctccag ttactattgg
     1801 agataatgtt tggattggcg ggggtgctat tatcatgcca ggtgttacta ttggagataa
     1861 tgtagttata ggtgcaggaa gtattgttac aaaagatatt ccaagtaata tgattgcata
     1921 cggaaatcca tgccgtatta ttcgagaaaa taaataaagt taaattttct tgcaaaatct
     1981 caaaaaacac ctttcttact ttatacatag ggtattttct aatactttca catccatatt
     2041 catttgttaa atttttgtgg aatgtagttt ttatcacttc tatcttacat ttccaccatt
     2101 tttttataaa agtcattcct ttatagattt atttataata gtatatattc tatttgatgt
     2161 ttgaataagc acttattttt aagtaaaata ctaactttta ggttagtatt ttgctatagt
     2221 atgatgatta agtaagaata aaatactata atacataaag cttaataaat ataattttta
     2281 ttttacttag atattttctt ttgattagca atcaaaagta gttataattt ttttaattaa
     2341 taagagtaac atttatttta tgaaagagga ttatattaat atatatttaa ataggaaaaa
     2401 ttgtaaaaag cataaaaata atataattaa ttaatgagat aaaagtggat aaaaatattg
     2461 ggagtttaat ttttgtgaaa ttaaaa
//
LOCUS       DAELJO010000344         2456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.346, whole genome shotgun sequence.
ACCESSION   DAELJO010000344 DAELJO010000000
VERSION     DAELJO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.346"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            288..2192
                     /locus_tag="KPB88_003861"
     CDS             288..2192
                     /locus_tag="KPB88_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0056088.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAESRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         1034 a          259 c          428 g          735 t
ORIGIN      
        1 aacaagacaa ataaaaatat tttaacatgt aagatatagt atgaactatt atactaatgc
       61 ttactttata atttgaacat attagaataa gtcttatttg aaaattttag tgtattagag
      121 taagtattat tttaccagta aaaaatattg agaactatat gtagatttgc ttaaaaagga
      181 atttacatat agttcttttc gtataataaa tagtacaatc ttatatggta aagataaagt
      241 gtgatattaa attttattat catccaaaat ctaaggagga gtctcttatg aaaataaaat
      301 ttattatagt agcatcaata atatttttaa tagtattact tgtggggtgt caaagtacta
      361 aagacacaga aatcaatgta ctcgccacaa gtgatttaca ttcaattatt ccagataatt
      421 taatttccta tgtagaagag gagagaaaat atgatgagaa tctacttatg gtagatgcag
      481 gagatttttt tgacatgcaa agtggagaga tgaataagtg gtttactggt caaaaattag
      541 taagatttaa agatgggata ccagagtaca aaaaaatagc tgaatcacgt gaaggagaag
      601 ttccattggc taaaaaaatg gcaaagctga agtatgatgc agttacattt gggaatcatg
      661 aatttgtatc taatgacaag cagagccttg ataaattggt ttctgatttt aaaaataaca
      721 atataccatt agtatcagca aacgtatatg aacaatctgg agaaaactat gtaaagccat
      781 atgtaatgaa aaatataaat actgacaagg gaactgtaaa ggttggtata cttggtctga
      841 ctataaaaga agtaggggaa cgttcaaggt ttaaaagttt tgaacaagat aaaaaggcta
      901 gagaacttgg ggaacaacct caatacaaag ggaaattata tgcaaatgat ttagtagaag
      961 atgcaaataa gtgggtaaag gttatggaaa aaaaagaaaa gccagatata atagttgcca
     1021 ttgtacattc aggtgaagag ccaaaaaatc ctaaaaatcc aggaaataga ataaaagaac
     1081 tagcaacaac tgtagaggga atagatgcta ttgtagcagg tcatacacat gaaaaaatag
     1141 aacagcatac atataaaaat aattctggtg aagaagtaat agtaacacag cctggagctc
     1201 atggtaatag tatatcaaag attaatttta agttggataa gaagaatgat aagtggttta
     1261 taaaagacaa atatagtgaa ttaaaggttt ttggaaagga agtaaatata attacaactt
     1321 ctggtctgaa tccaaaattt tcagatgaaa tggcagttaa tttgtttaat gaaagagata
     1381 gagatgttca acctatcttt atagatactg gagatttttt aaatactaat acaaaagaaa
     1441 tgactaagtg ggctaaagag tggcaataca atgaaaataa aggcatctat aaaaaaataa
     1501 atgaatggcc aattgtatat cttggttatg atgctgtagt acttggtagc catgaattta
     1561 cagaggataa agagactttt gaggaaaaaa aatctacatt ggattatatt atagaaaact
     1621 ttgaagaaat gaagatacca gtattatcag ctaatatata tgatgaatct ggagaaaatt
     1681 atgtaaaacc ttatataatt cataatgtag aaactaggga aggaaatata aaggttggag
     1741 tattaggtct tactactact gataaggatg aagaagactt aagtagctca agcttaagca
     1801 aagactcaaa gacttcgaaa attcctgaag aaacaggaaa gttatatact aacaatctag
     1861 taaaagatgc aagcgaatgg gtaaaagtta tgcaaaagga aagtcctgat gtgattgttg
     1921 cagttgtaca ttcagataat aatagtactc taaaaaatcc aagtagtgaa gtcaaaaaac
     1981 ttgcaatgtc agtagatgga atagatgcta ttgttgtagg tcatacgaaa aataaggtag
     2041 aagaacatat ttacaaaaat aagtctgggg atgaggttat agtaacacaa tctggagagg
     2101 attgttattc aaaaataagt cttgaactaa gaaaagaaaa tggcaagtgg aatatcataa
     2161 acaaatatag taaagcaata aaaatgaagt gaatttattg atatataaac cattattaat
     2221 actgattcta caatataatt aacactgatt tttataatat aataaatact gattctataa
     2281 tataattaac actacttttt ataatgtcat caacactgat tctataatat aattaatact
     2341 acttttttaa atataatcaa tactgattcc ataatataat taacactact tttttaaata
     2401 taatcaatac tgattctaca atattattaa tactactttt tataatataa tcaaca
//
LOCUS       DAELJO010000345         2430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.347, whole genome shotgun sequence.
ACCESSION   DAELJO010000345 DAELJO010000000
VERSION     DAELJO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.347"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1382
                     /locus_tag="KPB88_003862"
     CDS             <1..1382
                     /locus_tag="KPB88_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0056089.1"
                     /translation="IKEIETLYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVN
                     FLKARKYADLLKDMQVSSLCNIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQK
                     YRASNNITKILIKTRNYPVAINNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKS
                     NEKSFEQSICYLDRAKNILNMIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKK
                     LLEKSKNAKNYNYVKLAYKSIYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYID
                     SLNYRHEYIEKEINNMRKVKLDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFM
                     IDVDYFKEYNDTYGHYNGDIALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKK
                     SKIFGRKLCRIVKKFLNNNLTVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRC
                     LHYHDFKNF"
     gene            1409..>2430
                     /locus_tag="KPB88_003863"
     CDS             1409..>2430
                     /locus_tag="KPB88_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0056090.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVF"
BASE COUNT         1065 a          183 c          357 g          825 t
ORIGIN      
        1 taattaaaga aatagaaacg ttgtatataa agataaaaga aattaacgat tttaattgta
       61 aaaaaatata tttttttatt attggtaaaa tatatttttt gtttttggat gatgctctaa
      121 ttgctaaggt aaatttttta aaagcaagaa aatatgcaga tttactaaag gatatgcaag
      181 tatcttcact atgtaatata aagataggtg agtgtgaatg ttcgtttgaa aattatgaat
      241 gttccatgga atatttcaat gaagtaataa gcaataaaaa gtatagaaat gtaaatataa
      301 ttcaaaaata tagagcatca aacaacataa ctaaaatatt aataaagact agaaattatc
      361 ctgtcgcaat aaataattta gcaaaatctg aagtatatct agaaaaaatt aaaaatcata
      421 gcttaaaaga agtagagaag tttgatttat ttataagttt agccatgtat tatgcaaaaa
      481 gtaatgaaaa atcttttgaa caatcaatat gctatttaga tagagctaaa aacatattaa
      541 atatgataaa aaatatagaa gaatatataa tatatgattt agaaataatt tatcaccagg
      601 caaagatata ttatatcttt ggatattatg acaatgcctt aaacacaaat aaaaaactcc
      661 ttgaaaaatc taaaaatgct aaaaattata attatgtgaa gttagcatac aagagtatat
      721 atttgtgttt tgaaaagatt caaaattatg aaatgagtat gaaatacttt aaaatgtatt
      781 atgagttaaa aatgatgtat ataaaagctc gaaatagaaa ttatatagat tccttaaatt
      841 atagacatga gtatatagaa aaagaaataa ataacatgag aaaagtaaaa cttgatttag
      901 ataaaaaaaa gtatgttgac catttgacaa gtgcatataa tagaactttt ttgaacgatt
      961 ttttagaaaa acaagaagtt agtgaatatt gcacagcatt tatgatagat gtagattact
     1021 ttaaagaata caatgatact tatggacact acaatggaga tattgctctt aaaaatataa
     1081 ctataataat aaaaaaatat ctaaaaaaag acatgcttat gataaggtat ggtggagaag
     1141 aatttctgat attatctgta tgtaaagatt ataaaaaaag taaaatattt ggaagaaaat
     1201 tatgtaggat tgtaaagaaa tttttaaaca ataatttgac agttagtatt ggcattgata
     1261 catgtaaaaa tagtagtata gatataaaag aaataattga aaatgctgat aaagctttat
     1321 ataaagcaaa ggaaagtggt agaaatagat gtttgcatta tcatgatttt aagaattttt
     1381 aactgttgaa ataggattgg tgatttactt gaaaaggagt ttaatagaag atttaaagag
     1441 aaatataaac aagatagaat acgatatgtt gactaaattc tatctaaatt tgacagattt
     1501 aaactacatc ttggattatc acaaagacct tttaaataca aaattagaag ataatagtag
     1561 agtctatact ttatatttaa ttagtcttat aaactatata aaaaaagata gagaagcatc
     1621 atacaactac ttgatagagt cattaaagta tattaaaaaa ttgtcattaa aagatgattc
     1681 agatataata tctaaaattt atatttatct tactttatca gctatttcta taagaaaata
     1741 tgataaggta aataattttt atttctccgc aaaaaaaatt ataagacaaa atcatttaaa
     1801 tgagttgttg gtattgttaa atatgagttt atgtgtggaa ataagtgctt tatatcagac
     1861 caaaacggat gtaatagctt tagtagaaga agtgtctttt tttaaaactt taaaaaataa
     1921 agctcttgta agtagggcaa attatatatt tgggagagtt tatctatatt tatttaataa
     1981 ctttataaaa agtatggaat acttaattga atgtctaaaa ttggctcaag aaaataattt
     2041 atactctata gaggcttttt gtaaatgtat aataagtttg tgttatttag agagtggaaa
     2101 taatgtagaa gctataaaat acataaataa tgtattagat gttgcacaca gtaataactc
     2161 aatatcagta actgaaagag tgtctataaa aatagacctt atatgggcat atttaaaaga
     2221 agatatgaat aaagaagcag aagttatttt attaaagact ataaaattaa tgaaagttgt
     2281 tgaaggtgta tataaggatt atttatattc atttatattt ttatattcag ctgaaattga
     2341 gttaaagaaa gataattgta gttttgaaaa ggtaaacacc tatttaattt tatcaaaaaa
     2401 tatatataaa agttttggag atgtttttat
//
LOCUS       DAELJO010000346         2423 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.348, whole genome shotgun sequence.
ACCESSION   DAELJO010000346 DAELJO010000000
VERSION     DAELJO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.348"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            83..1411
                     /locus_tag="KPB88_003864"
     CDS             83..1411
                     /locus_tag="KPB88_003864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056091.1"
                     /translation="MVLKVGLNPKNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNK
                     NIYFKGLNIFVVKQINSKINTNFISISIICLMLFLTIGILSTGFGFKSALNAGLKGAT
                     PFDASATMIVHEKCKVKNIKESFEKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSG
                     RVDFEVAYIKSSQYNEIRKLRGEKSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKI
                     DNKAYNIKNKKVIKDNLRTDFTETNALTVVVNDKLCDSMEISSSIVNINFASDNNEKS
                     QENFDKRLKSKYSSTSFYEVGTVSGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSM
                     AVLALQQLSEASDSIDRYVSLKKLGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGI
                     QVANNFISRLNKPDIGASSLMTVGILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            1488..2159
                     /locus_tag="KPB88_003865"
     CDS             1488..2159
                     /locus_tag="KPB88_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0056092.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            2156..>2423
                     /locus_tag="KPB88_003866"
     CDS             2156..>2423
                     /locus_tag="KPB88_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0056093.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNF"
BASE COUNT          976 a          225 c          399 g          823 t
ORIGIN      
        1 agaggatata aaatttaaaa gtccatttgt atatacaatt acatttatat tatgcattat
       61 ttccttaatt attgcatata gcatggtttt aaaagttgga ttaaatccta aaaatcctaa
      121 gtttattatg tcaatagtat taggtgtaat tggaactata ttattcttct atagtttagc
      181 aggctttgca ttatatattg ttaagaagaa taagaacatt tactttaagg gattaaacat
      241 atttgtagta aagcaaataa atagtaagat taatacaaac tttatatcaa tatctattat
      301 ttgtttaatg ttatttttaa caataggaat actatcaaca gggtttggtt ttaaatcagc
      361 tttaaatgca ggactaaaag gtgcaactcc atttgatgca agtgcaacca tgattgtaca
      421 tgaaaaatgt aaagtgaaga atataaaaga atcttttgaa aaggttggaa ttaagtttaa
      481 taaaggtgat aaagttgctt actatgatga atatataaat ggtaataaac tttcagatgt
      541 tatgcaagga caatattctg gtagagtgga ctttgaagta gcatatataa aatcttcaca
      601 gtataatgaa ataagaaagt tacgaggtga aaagtcaata actttatcaa aagatgaagt
      661 attaataaca tctaatgcta cagaggtagt accttcgata gaagaataca tgaaaaataa
      721 taaaattata aaaatagata ataaagcata taatataaaa aataaaaaag taataaaaga
      781 taatttaaga actgatttta cagaaacaaa tgctcttact gtagttgtaa atgacaaatt
      841 gtgtgatagc atggagatat cttcaagtat agtaaatata aacttcgctt cagataataa
      901 tgaaaagtca caagaaaatt ttgataaaag gctaaaatca aagtatagtt ctacaagttt
      961 ttatgaagtt ggaactgtat caggtggatc aagaaatgct atgtataatg ataataatgg
     1021 tatgacttct acaatactat ttgttggaat ctatttagga atagtatttt taataagtag
     1081 tatggcagtc cttgcacttc agcaattaag tgaggctagt gatagtatag atagatatgt
     1141 atccttaaaa aaactaggag caagtaaaaa tagtataagt aaaactatat ttacacaaac
     1201 tctaatgtac tttagcatac ctgttggact agctttagtt cactcagtag taggaataca
     1261 agtggctaat aattttatat caaggcttaa taagccagat ataggagctt catcacttat
     1321 gacagtagga attttaatgt tagtatatgc agtgtatttt tatacaactt atcaagggta
     1381 taaaaatata gttataaaaa agcttaatta atacaactaa ctaaaatctg tttacagtaa
     1441 aaatcatact gtaaatagat tttttgaaaa taaagggtga aaaattaatg gaaaaaataa
     1501 ttatagtgga agatgaagaa gtaatcagag atgaattaaa aagcttttta gagaggtatg
     1561 gatatgaagt tatggctcct actgaatttg ataatatagt agagtttata ttaagtgaaa
     1621 aacctaattt agtattactt gatataaact tgccaatata tgatggatat tatatatgta
     1681 gagtattacg taaagaatcg aatataccaa taataattgt aacaagtaga aacactgata
     1741 tggatgaact aatgagcatg aatttaggag cagatgattt tgtgacaaag ccatataaaa
     1801 cacaaatact tttagcaagg attgttgcat tgttaaaaag aagtggatta aattcgaatg
     1861 taaataatac tttaatattt aagggtattg aattaaattt atttaatgca acactcagat
     1921 ataaagataa agaaatagag ttaactaaaa atgaatttaa aatactatct tatttagtgg
     1981 agaatcaagg ttcaatagta tctagagatt catttatgga gtatctatgg cacacaaatt
     2041 ggtttgtaga cgatagaact cttactgtca atataacaag gctacgtaaa aagctagatg
     2101 aaataggcat agaaaatttg atagagacaa gacgtggttt agggtatata atatcatgag
     2161 attaagagag tatataggtg ataaagttgt ttttctaggt actaatttaa ttatattttt
     2221 aattgcatca ggtatcatgc tttatagtaa aataaatgtc gttttaatat ttatgttatt
     2281 ttgtatatgg tttttaccat tatttactta tatgttaatt gattatttac aatataagaa
     2341 gtactttaat tctatagata atattttaga tgctttagat aaaaaatatt tgttgccaga
     2401 agttattgat gatgtaaatt ttg
//
LOCUS       DAELJO010000347         2422 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.349, whole genome shotgun sequence.
ACCESSION   DAELJO010000347 DAELJO010000000
VERSION     DAELJO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.349"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..1103
                     /locus_tag="KPB88_003867"
     CDS             <1..1103
                     /locus_tag="KPB88_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056094.1"
                     /translation="KTIKGDSNELDHININFGYALNGYINSYYTINGSNKTKKTELIK
                     LQKDSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEKGVFPSEGSDK
                     EIEYEKLDMNNFTRTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYATSDSSENNKEY
                     SSTIDIIKINPENKKIKTIKSINLSNYFSEKYQNIENIGVSYASKYEDKMYILVNVLN
                     TNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLYMVMSNDYDNSS
                     ITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRLNSILSWDDKF
                     MLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1112..1924
                     /locus_tag="KPB88_003868"
     CDS             1112..1924
                     /locus_tag="KPB88_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056095.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1935..>2422
                     /locus_tag="KPB88_003869"
     CDS             1935..>2422
                     /locus_tag="KPB88_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056096.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     EN"
BASE COUNT         1039 a          196 c          367 g          820 t
ORIGIN      
        1 taaaaactat aaaaggtgac tctaatgaat tagaccatat aaacataaat tttggatatg
       61 ctttgaatgg atatataaat tcatattata caataaatgg aagcaacaaa accaaaaaga
      121 ctgaattaat aaaactacaa aaggatagta agaacacaaa taatttaatt gatgtatcta
      181 gtggttcaga tttaattgct catgttggat ttgaaatgaa tgaggagtca aaagatataa
      241 actttgtgat agatgagaaa aaagaagatt tagaaaaagg tgtatttcca tcagaaggta
      301 gtgataaaga aattgaatat gaaaaattag atatgaataa ttttacaagg acaagatata
      361 aagtagataa aagtacagat gattattatg caaagatact agcatcaaaa agatataaaa
      421 aatacttata tgtatctgta ctttatgcta caagtgattc atcagaaaat aacaaagaat
      481 attcaagtac tatagatata ataaaaatta atccagaaaa taaaaaaatc aagactataa
      541 aaagtataaa tttaagtaat tatttttccg aaaaatacca aaacatagag aacataggtg
      601 taagttatgc tagcaaatat gaggataaga tgtatatttt agtaaatgta ctaaatacaa
      661 acataggtga taatagtaat tctaaatttc atttacttga gtataatctt gaagaaaata
      721 aggaaagcat aatggaatta aaaactgata gcaaaatggc tttagcaaaa gcaaagttag
      781 aagctaataa attatatatg gtaatgtcga atgattatga taatagttca attacagagt
      841 taaatttact tacctataat atattagata aaagcttaga tgaagataaa tatgttttag
      901 ataatagaga aaaaagagga attagaagta ttgaaattga ttcaaataaa atcgttgcat
      961 atacatcaag gctaaactct atattatctt gggatgataa atttatgcta tatataataa
     1021 ataaaaattc taaggaaatt gtatatgaag gagaattaca atttaacagt tcaaatagta
     1081 tgttagaatt tgaaataaag taggtgagtt gatgaaagat ttaataagat tattcgaatt
     1141 tgaattaaaa agaaatataa agaattatat atttataata atttgttgtt gttcttttgt
     1201 aattttaaac atagtaaaaa atttaaatga ctataattac ataatagaaa atgcagttaa
     1261 aagtgaacag ttaacaaaaa tcggaaatac gatagaaacg gtaaatgtta caggtttctc
     1321 aagctttaga aatataatgg gttctacaga atcgtggttt atgtttggta taattgcatg
     1381 tatttgctat gcttttttta tttggtatag agactttaat ggtagaagta aatctatata
     1441 tacacttata atgctaccta aaaatagaat caatatttat atatctaaat tattaaatat
     1501 attatttcta gtgtatagct atacagtagt acttacaata tctttgttta ttgcaagtaa
     1561 gttgttgcct agacatatgc ttggtaatgt cacaaactat ggttttgtac aagaaacaat
     1621 atatgagtta aaaatgttac ttccatatag ctttgaaata ctatttgtag agtatatatt
     1681 tttattgata ggatttgtat ctgtagtatt tacatcaatt cttataaata aatctattta
     1741 taaagtaagc actttaataa gttttctatt tttagttata gaaattttag tgtttataat
     1801 ttctatagaa tttattatac cctacgaata cagtgatgta tttactgttc tatattcaag
     1861 tataaacata tttgtatgta gtcttatatc taataaactt ttgaagcaaa agattgactt
     1921 ttaggaggga tattatgaaa gcaaaaaata taggaaaata tacaatatgt tttataatag
     1981 tatgtttttt aatgattact atgaccttaa tattgagtta ttttaataag gataaactat
     2041 ctgtaaaaac tttaaaaggc aatgtaaaag atatagggag agtaagtgta gtatatacac
     2101 ctaatataag caaattttct tatgaggagg taattataag taaaaatgga gtaagaaata
     2161 atacaaaccc aaaatcaaga attagaaatg attatttaaa tattgacaaa aaatacagtg
     2221 actttgttaa tgataaagga aaagaattta tatataaaga tgatttgaat ataggagaag
     2281 cgtatacctc tgttaagtct gatttagtac aaggaatgaa tgctttaaat gattgtataa
     2341 aggtttcaaa taaaaattta aaaagtgggg tagaagaaaa ttttgatatt aatttaaata
     2401 agtctgattt taaagaaaat ag
//
LOCUS       DAELJO010000348         2415 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.350, whole genome shotgun sequence.
ACCESSION   DAELJO010000348 DAELJO010000000
VERSION     DAELJO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.350"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..686)
                     /locus_tag="KPB88_003870"
     CDS             complement(<1..686)
                     /locus_tag="KPB88_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0056097.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGILVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIAGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVKGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTP"
     gene            complement(726..1073)
                     /locus_tag="KPB88_003871"
     CDS             complement(726..1073)
                     /locus_tag="KPB88_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056098.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1291..2247)
                     /locus_tag="KPB88_003872"
     CDS             complement(1291..2247)
                     /locus_tag="KPB88_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG0056099.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPIRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          819 a          362 c          280 g          954 t
ORIGIN      
        1 ggagttctat caaatttgag ttcaaaattt tcatcttcaa agtagggaat ataatataaa
       61 ggtgttttga gagcaggtaa tttattatat aattcttcaa gaatatcgat atttttaggg
      121 gagttaaaga aaaagcattt acatacattc attaaatcca cagcatctga aagtgagtta
      181 tgtttaatat ctccttttac agaaaaaatt tttttcatat tcagtaaaga tatttgctta
      241 gataagattc tattattgta tgtaatatgt tgagatataa aaggttgaat gtcaatcatc
      301 atattaaata actctaagca tttacctgta tatttataca ctctaaatga agttagaagg
      361 ccagctatat caaaatcacc ccaattatat attgtaacat catcaaattt acaaaaccat
      421 ttataaaaat catccataac atataaaaga ttaggagcat tattgatatc tagttggttc
      481 aatttggtta aatttttaca tttatttgaa agtattttat tgtattttgg cttaactaaa
      541 gagtaataat tatctattgt attaaattcg ttatcaacta aaattccacc aatagaaatt
      601 aattccgcac cattatattc ttttggttta tttttattat catctagtat attaaattca
      661 aagtctataa ataacttgta tttcataaat ctaaagcaca tcctcaggta aattatttat
      721 tgagcctaag catcataaga atcaatagat ataatgactc taaacatcaa aggttcatta
      781 caacttttat ttatttcata atttaattca aataaatctt ttaagatata atttataagt
      841 attctttccc aacccatttt taaattaact ttagagtcta ttaatatatt ttctatcttt
      901 ttaaatattt ctatatttgc tggtattata ctgagctcaa tattactttt aaacgggtta
      961 gactgtaaat ctgttataag tatatttttt aattcctctg taagttcaga aattaaacta
     1021 tcacctatgt atttttttat gatttcctct ctaatttctt ttaaatttag caatattagt
     1081 acctgctact tacatagtag cttttccttt ccttttaata ctattattca tttttgaatc
     1141 ttatttagat atattttaag attaaaagtc tttagctata taatttttta cccattttta
     1201 acttatatta acatattaca ggcttatatc tccaccctgc ttacattgag gatggagtat
     1261 attgcctatt ttgataaaaa tgataggttg ttatttgcct attccttttt ctgataattt
     1321 ttctaacata tctttggtca ttttatctaa atcataacta aacttaaagt tccattcttt
     1381 tactgcacta ctactgtcta gagagttagg ccaagaatca gctatacttt gtcttatagg
     1441 gtctacatca tattctatca caaaatcagg aatatatttt tttatagaat cagctatttc
     1501 ttcaggagaa aagctcatag cagtaatatt aaaagcattt ctatggatga gtttatcagc
     1561 tggagcttcg attaaatcca ctattgattg aagtgcatca ggcatataca tcatatccat
     1621 ttttgtacct tctgctatgt aagatttata tctcttattt tttaatgcct cataatatat
     1681 atcaactgca taatctgtag ttcctccacc aggaggcgta acataagaaa taagacctgg
     1741 aaatctaaca cctctagtgt ctactccaaa ttttgagtga taataatcac atagtaattc
     1801 tccagaaact ttagtcacac catacatagt attaggtctt tgtaatgtat cttgaggagt
     1861 catatttttt ggagaatttt caccaaaagc agcaattgaa ctaggtgtaa aaaatttaca
     1921 atccaattct ttggctattt caagaccatt tattaatcca ttcatattta agttccaagc
     1981 ttctagaggt ttactttcag caactgcaga aagtaaagat gctagatgaa ttatagtatc
     2041 tacatcatat ttttttgcaa tttcaaaaaa acgattctta tctgtaacat ctaatatctc
     2101 aaatatacca gattcacata ctggattacc ttcttttact ctccttgaac tagcgattac
     2161 attttgttct ccatactcat ttctaagctt tattgttaat tctgaaccta tttgtcctaa
     2221 tgcacctgtt ataagtattt ttttcaattt tacaacctcc taagggttta atttatgtag
     2281 tactacttat ttttatttta gcaaatcttt tgtttaaata caaagaatta aagataataa
     2341 tatacgactt aattgaataa agtgcagtaa gtgcctaaaa atttaaagct aatatataat
     2401 ggtctattgt ataac
//
LOCUS       DAELJO010000349         2393 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.351, whole genome shotgun sequence.
ACCESSION   DAELJO010000349 DAELJO010000000
VERSION     DAELJO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.351"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            150..443
                     /locus_tag="KPB88_003873"
     CDS             150..443
                     /locus_tag="KPB88_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0056100.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            424..1065
                     /locus_tag="KPB88_003874"
     CDS             424..1065
                     /locus_tag="KPB88_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG0056101.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYDRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1309..1641
                     /locus_tag="KPB88_003875"
     CDS             1309..1641
                     /locus_tag="KPB88_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG0056102.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            2183..2347
                     /locus_tag="KPB88_003876"
     CDS             2183..2347
                     /locus_tag="KPB88_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056103.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          923 a          205 c          377 g          888 t
ORIGIN      
        1 attatataaa agcgacttaa gtaaattgag aatcttaata tgaaaatttt caataactat
       61 ttagaaaatt ttctaaatag ctattgaaat aaaatggatg ttatgttata ttctatttag
      121 atgaaattct aaatagcgag gtgatggtgt tggggtttcc agaaacattc aaagctcttt
      181 cagaccctat aagaagagaa atattggtta tgttgaggga gaataaaatg tcagcaggag
      241 aaattagtga aaaatttaat atgacgaatg ctacaatttc ttatcatcta tctcaactaa
      301 aaaaggcagg actcatattt gaaacaaagt ataagaactt tattttctac gaaataaata
      361 tttcagtttt cgaagaggtt atgctttggt tttcacaatt tatggagaag ggagaaaaag
      421 ataatgaaaa aagcgataaa taaacaattt attttatcta ctttaatatg ttttatacca
      481 tttattgtat caatatattt ttatgataga ttaccaaatg aagttgcaat tcattttgat
      541 aattatggaa atccagataa ttatgctcca aaggttatag cagcttttgg tgtaccattg
      601 ttaatgctat gtatccattt gtatacatgg tttagacttg agaatgagcc taaaaaagct
      661 aatttttcaa atgttataca aaatttatca aaatggggag ttgctatact ttcagtaatt
      721 atacaaataa tgatggtttt atattcaaca ggagttagtt tggagtctaa tttttataca
      781 ggattaattt taggaataat agttatttta tttggaaact atcttcctaa atgtaaacga
      841 aattactcag taggaataat tttaccatgg actctgaatg atgaagataa ttggaataag
      901 acacaccatt tagctggatg gatttggctt attggtggaa tattattaat tattaatgca
      961 tttattaata ttccttttta taatatcttt gtaatctttg taattgtaat actaccattt
     1021 atatattctt atttgatgta taaaatttca ggtaaaaata actaatataa ttaatcacta
     1081 taataatgtt tggataagaa taatatattt cagaactgag ataggtaata aagaaattac
     1141 taagatttct attttagttc tgaaaaaatc ttacttatat gataacttat tgagatttca
     1201 aaaaataata taaattaggc taatgaacat aaaatgaaaa tttgtattta atattgaaaa
     1261 tataagtaca gtgtgatatt cttataagaa aaacatagga ggtagaagat gaatactaga
     1321 gaaatgaaag aatatagggg attttacaat aaaaaattaa gcgaaacaga tgagtttttt
     1381 agagtatttg gaagtcttga tgataagact tattcagaag gggctataag taaaaaaaat
     1441 aaggaattaa tgggactagc tatttcaatt ttaagccgat gtgatgagtg tatttgttat
     1501 catattgagg gttgtattaa ttctggagca agtaaagatg aaattataga agctataaaa
     1561 ataggtgtaa ttggtggcgg gtctattaca tatccaaacg caagatttgc aataaaaatt
     1621 ttagaagaat ttaatatata attttgatag acgtagttta tttagactaa taataatagt
     1681 ctatttaagt ctttgaaatt aagctttatt ttctaagttc acttcttatt aagtttttag
     1741 aattgcatat ataaatataa atttttaaat aatatagtag ttttaaatta tttacatcat
     1801 atattgtatt gagaatagta tattgtcatt attaaatctt ttatacattt tttattttaa
     1861 ataaattgaa tattaaggtg attatattta catatttgta tttttatgaa ctagaatatt
     1921 ctttataaat tcatagcagt tttaatagat aactttttca gagagattaa acaaagtttt
     1981 atttactgta ttctattatc agtttcctac aaattaaatt gtctattttt atttatagag
     2041 tagaataaac tgtttttgtt aagaattttg aaggtaaagc tagtttaaaa aaataaaggg
     2101 actaatttgt ctaacgttta ttggtgaaaa ataagtctat agaagttata ataaatatat
     2161 aaaaatgtat gagaggtgtt atatgtttgg taaaccaaaa gaaagtgtta gatttttaga
     2221 ctttaatgat gtagcagaaa agtttggaga gaaaaaggct gttgcaattt ttgatgccat
     2281 agctggaaaa aaatctaaag aacatattat caaagatata gaaaaattag taaaaaatga
     2341 agtttagata tttataaggt atattgagct tgagggtagc tctttatacc ttc
//
LOCUS       DAELJO010000350         2377 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.352, whole genome shotgun sequence.
ACCESSION   DAELJO010000350 DAELJO010000000
VERSION     DAELJO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.352"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            230..607
                     /locus_tag="KPB88_003877"
     CDS             230..607
                     /locus_tag="KPB88_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056104.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            600..1622
                     /locus_tag="KPB88_003878"
     CDS             600..1622
                     /locus_tag="KPB88_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0056105.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYSYSKSELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNRVNLDCELCCDESVIYNCSFNDKKEYALALIKSIKLSKECNRGILLTDFNRTNLE
                     KRLESIISKKFKNSVFVVIILCLLSSITYFKFDIFISKPVIYSLPKPSVLNDEYIYNT
                     DIRVER"
     gene            1684..2277
                     /locus_tag="KPB88_003879"
     CDS             1684..2277
                     /locus_tag="KPB88_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0056106.1"
                     /translation="MLKKIKKVLSIACCLFILGGSISFADLENNNEINDKSVENPIYE
                     FNIQGYTFTYENAPQRIKDEYNKTCRELNITPSPSTEIFIPIDEISKYTDTVPLFEID
                     EFNVKYFGTRFEVTGAKTYTVGVSTYVGYGHTTSGNPVHLAQLLLKKSGYSIAIDSLF
                     GSSTYSKVRSFQSKYGLSVDGVVGLGTWNKLAQVTNA"
BASE COUNT          954 a          219 c          363 g          841 t
ORIGIN      
        1 tttttattaa atagttttaa tgtgtaaata acattctaca ctattttaat taaatatgta
       61 aaaagtaaaa tatttaaata gatatatttt aaaatctaac ttttaatatt gtaaaaaaat
      121 tagaaattta acaaaaagta caaaaattca catttttttc ataaagtgtt tgactaaaaa
      181 attcacatgt tgtagaatta agatagcagg agttttgagg gaggtgtaaa taatgataag
      241 aaaaatacca caagcagaac taaaagtaat gaaatttatt tggaaagtag atgttacagt
      301 gacatcaaaa gatgttattg aagctatgga acaaaaatat ggctggaaac aaaccaccac
      361 actgacactt ttatctaggc tggtaaaaag aggattctta gatgctcaaa aaatagacag
      421 atatacacat tacacaataa gagtaaaaca caaagattat ttaagttttg agacaaagga
      481 tttcctaagt aatatacatg atgactcttt aagaagctta atttcagcat tacatgatga
      541 cgaaaatata ggtaaagata agctagattt tctagaaagt tattttgata atttaaagga
      601 tgaataatta tatgctatat gtttttgaaa atcttataaa gacaactatt gtatgtagtc
      661 ttggtatttg tctattatta tttttgaaaa gatatttatt taagaagttt agtaagagat
      721 tcaattatta tatttggttg ataatagtct ttagaatgtt actttttcta tttaattata
      781 caatagtgta tgaagtaaaa gaacccaaag aaaatgcatt aggaaataat attacacaaa
      841 ttgatatttc tacagataat aatcttatgc tgtatgtagc ttatttatgg ttatttgtaa
      901 ctatagtcat tgcagtttat acatttataa agtatacaag atttaaaaat ctagttgttg
      961 atgtatctta tgatattgaa gataatgata taaactgtat ctacaaaaac ttgttaaaag
     1021 aattaaatat aaaaaagaaa attgaattaa gaggttctga tgaattgata agtccagcag
     1081 gtatgggatt atttaaatct tacatattct taccagacta ttcttatagt aagagtgagt
     1141 taacttggat tttgaaacat gaattaatgc attttaaaaa taaagatatt ttaataaagt
     1201 ttttagtttt gtctgttaga ataatatatt ggtttaatcc acttgtttat gtaatgagca
     1261 atagagtaaa tctagattgt gaattatgtt gtgatgaaag tgttatttat aattgttcat
     1321 ttaatgacaa aaaagaatat gcattggcac ttattaaatc aattaaacta agtaaagaat
     1381 gcaatagagg tattctatta acagatttta atagaaccaa tttagaaaag agacttgaaa
     1441 gtattattag taaaaagttt aaaaatagtg tttttgtagt tataatttta tgtttactat
     1501 cttcaataac atattttaaa tttgatatat ttatttcaaa accagtgatt tattcattac
     1561 cgaagccaag tgtgttaaat gatgagtata tttacaacac tgatatccga gttgaaagat
     1621 gatgttaaat tgtattaaaa aatgtaataa tttaaataaa agaaaatatt ttaggaggat
     1681 tttatgttaa aaaaaattaa aaaagtacta tcaatagcgt gctgtttatt catattagga
     1741 gggagtatat cttttgcaga tttagaaaat aataatgaaa taaatgataa atctgttgag
     1801 aatccaattt atgaatttaa tattcaaggt tatacattta catatgaaaa tgcaccacaa
     1861 agaattaaag atgaatataa caaaacatgt agagaattaa atataacacc ttctccatct
     1921 actgaaatat ttattccaat agatgaaatc tctaagtata cagatactgt tcctcttttt
     1981 gaaatagatg aattcaatgt taaatatttt ggaacacgtt ttgaagttac tggtgcaaaa
     2041 acatatacag ttggagttag tacatatgtg ggatacggac atacaactag tggaaatcca
     2101 gtacatttag cacagctttt actaaaaaaa tcaggatatt ctatagcaat agacagtttg
     2161 tttggtagtt ctacatatag taaagttaga agttttcaaa gtaaatatgg actaagtgtt
     2221 gatggtgtgg ttggattagg aacatggaat aagttggcac aagttactaa tgcttagata
     2281 atttattata aagctaaaaa gaaacatggg agtctggagt ctggaaatat tcatgcatat
     2341 tcttttaatg aaattttatt tttaaaatga caattaa
//
LOCUS       DAELJO010000351         2373 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.353, whole genome shotgun sequence.
ACCESSION   DAELJO010000351 DAELJO010000000
VERSION     DAELJO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.353"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            168..356
                     /locus_tag="KPB88_003880"
     CDS             168..356
                     /locus_tag="KPB88_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG0056107.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(516..668)
                     /locus_tag="KPB88_003881"
     CDS             complement(516..668)
                     /locus_tag="KPB88_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG0056108.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(766..1242)
                     /locus_tag="KPB88_003882"
     CDS             complement(766..1242)
                     /locus_tag="KPB88_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0056109.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETPFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1461..2222
                     /locus_tag="KPB88_003883"
     CDS             1461..2222
                     /locus_tag="KPB88_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0056110.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDSIVIYTRDK"
BASE COUNT          879 a          320 c          311 g          863 t
ORIGIN      
        1 tatatttact ttatataaaa ttatatatat aattttatat gataataaga aattcttcaa
       61 tatgtgtaag gcaaatatat atttctattg caaatttttg ttatttatga taatataaat
      121 atgtttttag tctttaaata gttatcattt taaggaggtg tgcgtagatg gcaaaagtat
      181 gtagcgtatg tggtaaaggt aaggtttctg gaaaccaagt atcacactca aataaacata
      241 ataaaagaac atggtcagct aacttaagaa gcgtaagagc gattatagat ggagctccta
      301 agagagtaaa agtttgtact agatgtttac gttctggtaa aattgaaaga gcttagtaac
      361 cacgtttaaa agcctacttc cgtaagtagg ctttttaatt taatcatttt tactctttct
      421 cataaatttt aggattatac ttgaaatcca ttttggtaat tttattgtta ctactttcat
      481 caatactcac cccacacttt atagttaaaa ctattttaac aaaaaaataa taatgctcca
      541 catccaataa gtattaatgc ggtaagtgct gaccaaatcc agccaggaag caacattgac
      601 agaattacag ccattcctgt tgctagacaa agtattccta gcaatacctt agacttgtta
      661 tttctcatgg aacacctcca catagaacct gtatctataa atattttcat attcaagttt
      721 atgcgacagt tttccatttt agaccaataa acacattatg taattttaat ttttcatact
      781 agaattatta ctgaactccg atatagattc tacaatatcc aaaattcctt tttttctact
      841 taacctttca ggtccactat ataaaacaac tggtataact ttaggaagtt taaaattttt
      901 atcttctatt tcttcaaggc tttttttcat aataaaactt ttccaaattt gttctctata
      961 cgatttcaat cttgggacaa tttcagtatc catttctgtt tgaaattcta acactatata
     1021 atgtgatact ttgccacttt tagaaaatac cttataaatt atatcaactt cttttccttt
     1081 atattcacat ataaaaggtg tttctaactc tatattttta agagttaatt cttttgctat
     1141 tgagacactt gtaaaatttt gaatcaaact taaaaaactt tctttatctg aatacattct
     1201 tctgtactct tctcgttttt cattgcttct ttttaaattc atacagttcc tcctagacta
     1261 tagaccctct attttttagt aaagatagtc tataaatttt attttttaat tttcattagt
     1321 gagtattgac agttgtgtgt aaaatatcca gtaaagatat ttttttatat ttttattaaa
     1381 agatataaat ttttatattt taatgtattg acatttacgt tgcgtaaaag tgtatattta
     1441 atttatagga ggtactactt atggaatata cagttcaaaa attaagtaaa ctagctggta
     1501 taagtactag aacacttaga tattatgatg aaataggtct tcttaaacca cttaaaataa
     1561 attcttcagg ttatcgtata tatggtcaaa atgaagttaa taaacttcaa caaatacttt
     1621 tttatagaga attaggaatt aacttagaaa atataaaaaa cattataaat tcaccaaact
     1681 tcgatagttt aagtgcgtta aaagaacacc ataataaatt actagccaaa agaaagcaaa
     1741 ttgatttact aatagaaaat gtaactaaaa ctatagctct gaaggaggga aaatacacta
     1801 tgacagatat agaaaaattt gaaggtttta aagaaaagat gattgatgaa aatgaaaaaa
     1861 attatggaac tgaaattaga gagaaatatg gtaaagatat tatagataca tcaaataaaa
     1921 aacttaaaaa tatgtctaag caagattatg aaaattggca aaacctaaat atcgaaataa
     1981 ttagtaaact taaaaaagct tttaaaactg gtgatgcctc tagtgaactg gctcaggaag
     2041 ttgcaaaatt acatcataaa tggttgagtt acacttggaa cacatactct aaagaagctc
     2101 attcctctct agcacagatg tatgtatatg atgaacgttt tacatcatat tatgatagag
     2161 aacaacctgg attagctaag tttttaaggg attctatagt aatatataca agagataaat
     2221 aatataaaat aataaaggtg gtgtctcaaa atgaactttt tagttcatga gacactcttt
     2281 tttgtatgaa atcagatata tttttatcat tttatcaatg aaaaacaggt ttctctctct
     2341 attttgagac agaatcttta ttgtataata att
//
LOCUS       DAELJO010000352         2352 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.354, whole genome shotgun sequence.
ACCESSION   DAELJO010000352 DAELJO010000000
VERSION     DAELJO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.354"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            29..184
                     /locus_tag="KPB88_003884"
     CDS             29..184
                     /locus_tag="KPB88_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056111.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            314..1327
                     /gene="aroF"
                     /locus_tag="KPB88_003885"
     CDS             314..1327
                     /gene="aroF"
                     /locus_tag="KPB88_003885"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0056112.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1349..>2352
                     /locus_tag="KPB88_003886"
     CDS             1349..>2352
                     /locus_tag="KPB88_003886"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG0056113.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKSVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     "
BASE COUNT          948 a          240 c          431 g          733 t
ORIGIN      
        1 ataaattata aaaatggagg ataatactat gaaaggaata ttaaaagaac taacagcaaa
       61 actttatagc tttttttact ttatgttctg gggcttttat tttagcgctg gctatttctt
      121 ttacaaaaat atatgtactt cctttctggt gaggccttat tttagtgatt ttgagcataa
      181 gtaaatttta taaaaatgat tattttatat atttatataa gatttagtgc ttgatagatt
      241 attttagaga actcattagt gagttctctt ttttatttta aatataaaat tataatattg
      301 ggaggtagta aaaatgatag tagtattaaa aatgggagca gataaaaatg aagttaaaaa
      361 attaattgag gctattggga gagaaggagt agaagttaat cccatagatg gaacagaatt
      421 aactgtttta ggtttagttg gagatactag taaaatagat gccaaaagaa tagaagcaaa
      481 taaggtagtt gaaaaagtta tgcatgtggt agagcctttt aaaaaggcca atagaaaatt
      541 tcatccagaa ccttcaatca taaatgttaa tggaatggaa attggttcaa agaaaattgc
      601 tatgatagct gggccttgtt ctgttgaaac agaagaccaa atagtatcta tagctaaaga
      661 tgtgaaaaaa tcaggtgcag gatttttgag aggtggagca ttcaaaccaa gaacatcacc
      721 atacgctttc caaggattga aatatgatgg attggattta ttaaaaaaag caaaagaaaa
      781 aacagggctt cctatagtta cagaaataat gtcaacacaa gatatagata tatttgaaga
      841 aaacgtagat gtaattcaag ttggagctag aaacatgcaa aactttgatt tattaaaaga
      901 attaggtaaa acaaataaaa caatactttt aaaaagagga ttatctgcta caattgaaga
      961 gtggctaatg tcagcagagt atataatggc tggaggaaat gaaaatgtag ttctttgtga
     1021 aagaggtata agaacttttg aaacatatac tagaaatact ttggatttaa gtgcaatttt
     1081 agctgtgaag aaacttagtc atttaccagt tatagttgac ccaagtcatg cagcaggtaa
     1141 atcatggatg gttgactctt tatctaaggc agcaattgca gttggagcag atggtctaat
     1201 aatagaagtt cataatgacc cagcacatgc actttgtgat ggaaaacaat cgattaagcc
     1261 aaatgaatat gatgaattga taagtgaatt aaagactatc gcatcagcag ttggtagaga
     1321 aatataatct ataaaaggat ggttaaacgt ggaagaaaaa ctattagtaa atttaaaaga
     1381 aaatagctac aatatattta ttaaaaaaaa tatactaaag aatataggag aagaaataaa
     1441 aaaaatatat tttggaaata agatttttat tgtaactgat gaaaatatag caaaattata
     1501 cttggataaa gtaaaaaata atttaagtga atctggattt gaaattaaat ctttaatatt
     1561 aaaatctggt gaagaaacaa aatcatttaa tactcttcct aaaatatacg atagtttact
     1621 tgactttaag ttaactagaa aagatttgat tattacttta ggaggagggg taataggtga
     1681 tttaggtgga tttgcagcag caactttttt aagaggtata agttttattc aaattccaac
     1741 atctttatta gcacaagttg atagttctgt aggtggaaaa gttggagttg accttgagag
     1801 aggcaaaaat ttagtgggaa gcttttacca accaaaatct gtgtttatcg acccaaatgt
     1861 attattatca cttccagaaa aattctatag agatggaatg gcagaagtta taaaatatgg
     1921 atgtataaaa gacaaaactt ttttcgataa acttttaagt ttaaaaaata gagaagaagt
     1981 aatgcaacat atagaataca ttatacataa atgttgcttt attaaaaaaa ctgtagttga
     2041 agctgatgaa aaagactttg gagatagaat gcttctaaac tttggccata cattaggtca
     2101 tgctattgag aaatactaca attttacagg ttatacccat ggagaagctg ttgccatagg
     2161 aatgtataag ataactttag aaagtgaaaa gataggaatt acaaaaaaag ggacatcaga
     2221 acttataaaa tcaattttaa tgaattataa tttgccttat gagattgaat taagagataa
     2281 taaatctata gtagatgcta tatctcttga taaaaaaaat ctaggtgata cattaaaaat
     2341 aatactttta aa
//
LOCUS       DAELJO010000353         2298 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.355, whole genome shotgun sequence.
ACCESSION   DAELJO010000353 DAELJO010000000
VERSION     DAELJO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.355"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(68..>2298)
                     /locus_tag="KPB88_003887"
     CDS             complement(68..>2298)
                     /locus_tag="KPB88_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0056114.1"
                     /translation="NHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNREFM
                     YQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKGIV
                     AYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTIWN
                     GEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHVLQ
                     TKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDLHL
                     FEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIEIT
                     ENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLSHN
                     LNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPMECK
                     EFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDFYN
                     KTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQKY
                     SNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQFY
                     SMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFVYK
                     KSLAMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKNLT
                     KI"
BASE COUNT          794 a          333 c          270 g          901 t
ORIGIN      
        1 atcctggaaa agtttttaaa aaaatttctt taaacatatt aatttctcca aaataaaata
       61 ctttgtacta gatttttgtt aaatttttta ttttatttac atctacatac ataccctggg
      121 caatcgacct tccatcaatt gcagaaaggt tacattgtgt atacataact gtgacaattt
      181 gaccatctcg ttttatgaat ctatataaaa atggttcaat tttatttgtg tcaatcatag
      241 ccaaactttt tttataaaca aagtctaaat catctggatg aacaattgct cttaaatttc
      301 cgcccatctc accaaaaaac tgttcttttg tataacctat aatatcaaac atttcttcat
      361 tcatataagt aataatcata tcaccaaata aatcgtagcg aattatacct acaggcaaca
      421 ttgaataaaa ttgtgataaa atattacttt catcaagatg tgtatttgtc attttaggta
      481 ttgaatatga ttcaaattca tttatatagc cagcagtttt aattaaatcc aaatgtaatt
      541 ttgctaagag taatttattt tctgaaatta tacaatttgc actaaagtag aaattgctat
      601 atttttgatg ttcagaagct tcatctgata aaacggcttc tccactaatt aataatgaat
      661 ctgattcagt attatgtggg gttatacttt tgtaaagaat agaaaatttt ttacctttaa
      721 tatgtttttc aaaatgttct attactttag gaaaagatgt aatttcagtt ttgttgtaaa
      781 aatcaaacca ttgaatgtct tcagtgacat tagctcttag ttttttcata tcttgcataa
      841 taaaaaatcc atcaaatatc ttctctatta aagattttcc tatatcagaa tattttataa
      901 acattttctc tgaattattc tctttagata gtaatttttc aaattctttg cactccatgg
      961 gttttgcata ataataacct tgggcgatat cacaatttat acttttaata aattctacat
     1021 gttgttctgt ttcaacacct tcacatactg taagcatccc aagagctctt gcaatgttta
     1081 ttatagagta caaaatggtt gtatttttaa aatcatttaa attatgagat aaaaatatcc
     1141 tatcaatttt gattatatca aaaggtagat tttgcaacat accaaaagat gaatatccag
     1201 aaccaaaatc gtccattgat acagaaaaac cataagaacg aagttcagct agttgttgaa
     1261 taattgtatt tgtattttct attaatatat tttctgttat ttcaatgcta aaatttgatg
     1321 tagagacgct gtacttttca gtaatacctt ttagcatact tggaaaatta gggttaaaaa
     1381 aatgaagacg agaaaagtta caacaaattg gaacaaaaac atcattgtta tcaatccatc
     1441 ttcttatatt tttacagttc tcttcaaata aatgcaaatc taattccgtt atcagaccaa
     1501 actgttcaaa taaaggaata aatttatctg gagtaagaag tcctttaata gggtgtctcc
     1561 atcttgcaag tgcctcacat ccaactatag tattattttt tatattataa agtggttggt
     1621 aaaatacttc aatttgttta gtttgtagaa catgtgatat ttctgataag aactccttat
     1681 cattttctaa tttattgtaa aaactattat ctttaaaatc aaatagaatg tatctatttt
     1741 taccttcact tttagccttt tttaaagcac tatatacttg gttgaaaagc atcaaagtag
     1801 tcatgtttgg ttcaccattc cagatagtgc tgccgatact aaatgtaaca ttggagtata
     1861 tttctttttc attaggaagt tttattttta attctcgggc gctctctata atagttgcac
     1921 ccatattatt taactcttta tttgatacat ttggaagtac aattaaaaaa ccttctcctt
     1981 ttaaataagc gactattccc tttgattctg gaataaagtg caaaaggttt tgagttagag
     2041 taattaataa attttctccg actagatatc catatatatc attaaataat ttaaagttat
     2101 cgatatttaa taacatgatt cctatatgta tatttttcga aatacagtca tcaatatatt
     2161 ggtacataaa ttctctgttt ggtattttag aaaaaaaatc tatttcattt aaggttttta
     2221 atttaaaatt tctactatct aattccttag ataatacttc taccttttta tttaacattt
     2281 ttatataatt atgatttt
//
LOCUS       DAELJO010000354         2282 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.356, whole genome shotgun sequence.
ACCESSION   DAELJO010000354 DAELJO010000000
VERSION     DAELJO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.356"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            84..407
                     /locus_tag="KPB88_003888"
     CDS             84..407
                     /locus_tag="KPB88_003888"
                     /inference="COORDINATES: protein motif:HMM:NF020435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBG0056115.1"
                     /translation="MLLVENKNDRSILAKESLEKLGAKNVKIYNMDTGSNVIYSVENG
                     KEHLTISNEKRFPNWNEIKYVRYKLMKPNKTIVQILPPKEEYINLHENCFHLWEIEDN
                     AVPTK"
     gene            494..691
                     /locus_tag="KPB88_003889"
     CDS             494..691
                     /locus_tag="KPB88_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056116.1"
                     /translation="MNNKVEINNIQQLNDALNKYDIPFGILSDVDRRICDWMATGGNE
                     DDAYIKQQYRYVENFINRFCD"
     gene            722..907
                     /locus_tag="KPB88_003890"
     CDS             722..907
                     /locus_tag="KPB88_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056117.1"
                     /translation="MVITEFKVTWQTTSEWLKGGVYSNIFNNLEEARAFKKNQENIKE
                     NYNVKLLSRAIVEIEID"
     gene            1062..1460
                     /locus_tag="KPB88_003891"
     CDS             1062..1460
                     /locus_tag="KPB88_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056118.1"
                     /translation="MQKDVWLYSWDDECFGSDEYESKEEAIQGAKEELRRFGEVSRLV
                     YVGQKEEVNIPNLNVEDALEHVQDRIDNEFMGYGEDWFEKIRVEDILILENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            1508..2233
                     /locus_tag="KPB88_003892"
     CDS             1508..2233
                     /locus_tag="KPB88_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBG0056119.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
BASE COUNT          969 a          195 c          461 g          657 t
ORIGIN      
        1 gaaagtaaag attgtgatac aacatatgaa aattggctag agaaggaaac aaaaaattat
       61 ttataaaatt tggagggaat aaaatgttat tagttgagaa taaaaatgat aggtctattc
      121 ttgcaaaaga atcacttgaa aaattagggg ctaaaaatgt aaaaatatac aacatggata
      181 ctgggagtaa tgttatatat tcagtagaaa atggcaaaga acatttaact atatcaaatg
      241 aaaaaagatt tcctaattgg aatgaaatta agtatgttag atacaaactc atgaaaccaa
      301 ataagaccat agtacaaata ctaccaccaa aagaagaata tataaattta catgaaaatt
      361 gctttcatct ttgggagata gaagataatg cagttccaac taagtaattt ataaaaataa
      421 agtcaaggta agtttgtgaa tgaaactaga atgttataga cttactttga cttataaaaa
      481 ggatgatttg aatatgaaca ataaggttga gataaataat atacaacaat taaatgatgc
      541 tttaaataag tatgatatcc catttggcat attaagtgat gtagatagaa gaatatgtga
      601 ctggatggct actggtggta atgaagatga tgcttatatt aaacaacaat atagatacgt
      661 agagaatttt ataaatagat tttgcgacta gggagtgaga ttatgaaaag gagtggtcaa
      721 tatggttatt acagaattta aggtgacttg gcagacgact agcgagtggc taaaaggggg
      781 agtgtacagc aacattttta ataatttaga agaagcaaga gcttttaaaa aaaatcaaga
      841 aaatataaaa gaaaattata atgtgaagct tttaagtaga gctatagtcg aaattgaaat
      901 agattaaata tgtgagttaa aaggaagtga ttctttgaaa aggataagat gcagttggtg
      961 tggcaaatta ttttatctta aggaaaaatc taaggatgtt tattgttgta aggaatgtag
     1021 gaagaaggct aataagaaaa ataaatagtg gaggtattaa tatgcaaaaa gatgtttggt
     1081 tatatagctg ggatgatgag tgctttggaa gtgatgaata cgaaagtaaa gaggaagcta
     1141 ttcaaggagc taaggaagaa cttagaaggt ttggggaggt cagtcgattg gtttacgttg
     1201 ggcaaaaaga agaagttaat atacctaatc taaatgtaga ggatgcttta gaacatgttc
     1261 aagatagaat tgacaatgaa tttatggggt atggagaaga ttggtttgaa aaaatacgtg
     1321 ttgaagatat attaatacta gagaatagaa taagcaaagt atttaaaaaa tggatagatg
     1381 aatttggata taaaccatat tggtttgttg ttagagatac ggaagaaata gaactaaatg
     1441 aggtagcaaa tgaaagttaa ttttgtaata gatggagagc cagttggttt taaggaggac
     1501 tataaatatg aatgagatta tgacaaatga aaatttaaga gttgtagcag atgatttagt
     1561 tacagtttat gaaactgata caggagagaa aatagttttt gcaagagaac tacataataa
     1621 cttagaagtt aagagacagt ttatagactg gattgaagat agaattaaac aatatggatt
     1681 taaggaaaat gaggattatt cagtttttca caaaaatatg aaaaactcaa ctggtgggag
     1741 accttcaaaa gaatatgtat tgaagcttga tgtggcaaaa gaacttgcta tggtacaaaa
     1801 caataataaa gggagagaaa taagaagata ttttataaga ttagaaaagt tgctcaatag
     1861 aaccttatca aattcacagc ttagtcaaat taatactatt gtgaatgaat cattacttaa
     1921 gatggaagtt aaacataata tccaaataga gcaacttaaa aaagagtgtt cagaatatta
     1981 tagaccaacc tctaaaacta aatatgatat atcttcttat ataaaagaaa gactaggtat
     2041 atcaaaagtt aatgaagaat ttgaattagt taagaaaaga actctattgg tattaggtgc
     2101 tgacaaatgg gaggatatcc cgaaagatgt attacttaat tcattaaact taatagatga
     2161 atcaattaga attataaaat ctgaaagaaa gacaaatcag attagtttct ttgaaaaaga
     2221 taatttctgt taataaaaag aaaaaaggag tgctttcaca ctccagtcgt caaaaatata
     2281 aa
//
LOCUS       DAELJO010000355         2270 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.357, whole genome shotgun sequence.
ACCESSION   DAELJO010000355 DAELJO010000000
VERSION     DAELJO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.357"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..265
                     /locus_tag="KPB88_003893"
     CDS             <1..265
                     /locus_tag="KPB88_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0056120.1"
                     /translation="NLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQ
                     IKRGIVECTGVFGNMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            253..1191
                     /locus_tag="KPB88_003894"
     CDS             253..1191
                     /locus_tag="KPB88_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0056121.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1575..1799)
                     /gene="secG"
                     /locus_tag="KPB88_003895"
     CDS             complement(1575..1799)
                     /gene="secG"
                     /locus_tag="KPB88_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG0056122.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          851 a          303 c          258 g          858 t
ORIGIN      
        1 aaatcttaac ataaaaggtt ataaattttt accttatgtt aaaaattcaa aagcaagtag
       61 agataactta tgttttaaaa taaacacaaa tattgatgag ccaaaaaaat acttagaaca
      121 aaatgagatt caaattaagc gaggaattgt agaatgtaca ggtgtctttg gtaatatgaa
      181 atctatttat ttgaaaaaaa ctaatggaaa tttaattgaa ttatcaaatc atagaatcac
      241 ggagagtaaa ccttggaaaa tttaatccta tcaataaatg ttgttcttcc tttgtttctc
      301 acaatgtcac ttggatatgt tctaaaaaaa ttaggtctat ttgatactca tactttaaat
      361 aaaatgaata atgtctgctt caagtctttc ttgccattat tattattttt taatatatac
      421 aatacagatt tagatggttc tcttaatttt aagcttatgg ctgtagctgt tataattatt
      481 ttttcaatct tcttaatctt agtctttatt attcctaaaa ttgaaaaaga taataagaca
      541 aggggtgcat taatacaagg tgtatttcgt agcaatttcg ttatatttgg tattcctgtg
      601 acaatttcat tatttggcga taagagtatt ggagtcacat ctttattaat tgcagttatt
      661 gtacctatgt ttaatgtatt atctgttgta attcttgaaa tttttagagg tggcaaagta
      721 aatataaaac atatgattaa aagcgtctta acaaatccac tcataatcgc ctcttttata
      781 ggattgacaa tgctaatttt aaatattaat atacctacat ttttacacaa aactattggt
      841 gatatttcta aaatagctac tcctttatca cttataattc taggcggctc ttttagtttt
      901 tctgctataa aggggcatat aaaacccatt atattgggcg tatgtggaaa acttatactt
      961 gtacctgcta tatttttacc aattagcatt tttcttggat ttagaaatgt agaattagct
     1021 tcacttatga taatgttatc tgctccaact gcagtttctt catttacaat ggcacaacag
     1081 atggattctg acagtgaatt agctggacaa attgtagtat ttacttctgg attttctata
     1141 attacagtat ttttaataat atttatttta aagcagaata atcttattta atgatatttg
     1201 ataacacaaa ttggtattat aagaaacata tataaaactg atttaatcgc gtaaaaacat
     1261 attattatac tatttttact atataatata actttgtgta tattatctca ttaaacatgg
     1321 tgaaagtagt gttgctatat attttttagt ttgaagaaaa tatataaata caaacaatat
     1381 ataaatacaa ataataaacg ccaacaaata gtcaatctat aaaataaaaa ctggactaat
     1441 catataaaat cattagtcca gcttttattt tacgtacttt ttatgttgat gtacttttat
     1501 taaaaactaa atttaatttt taaatgtcta gcaaatgcac taattaaata atggccagtt
     1561 attcaacaat atcactactt cttaactata agtaatgcca atgcatttat aaaaaataat
     1621 actgctgtta tctttgtaac tctagcaaga aatgcttgtt ttccttttgg tttaaagtaa
     1681 gcttggcttc cttctccacc aaattctgaa ggtaaagcat tcttagaatc ttgaggcatt
     1741 atgctaatta ctagtaaaat ccctagtact acttgcacac ccattaaaat gttgctcatt
     1801 cttgcccctc ctatctgttt attacttaaa tttcattact ctttagtgta tcacaataat
     1861 atagccattt caagactatt atgctataaa taacatacaa aaaaatatat tgttactata
     1921 atatattact atattctcaa tataaaaatg aataaaactt agaagagaaa actatttaaa
     1981 ttaatacata aattaacact taaaataatc tactctcatt gtatatatta taaatagcca
     2041 ccaaaataaa acaatcattt taaaatattg atataacttt taatttcaac ataatttaaa
     2101 ataaataatc cacatttagc aataatactt taatgacaat tatgattaaa tcaaaatatg
     2161 tcttcacttt tattttttaa taaaaacctc ctatgcaatt agataaaaat gagatagaaa
     2221 catttattca tattttttct gaatctatga ataaaattaa gtaataaaat
//
LOCUS       DAELJO010000356         2269 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.358, whole genome shotgun sequence.
ACCESSION   DAELJO010000356 DAELJO010000000
VERSION     DAELJO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.358"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..860)
                     /locus_tag="KPB88_003896"
     CDS             complement(<1..860)
                     /locus_tag="KPB88_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0056123.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKE"
     gene            979..2178
                     /locus_tag="KPB88_003897"
     CDS             979..2178
                     /locus_tag="KPB88_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG0056124.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
BASE COUNT          823 a          292 c          356 g          798 t
ORIGIN      
        1 aattctttgg ttgctaagga taaagatata tcttttaaat gacagtaacc tatatttctc
       61 ttaggtattg gctcttttat atctaattta aacaatttct cattttccaa ataatctatg
      121 ctaaattctt ctataacaca agacactcca agatttatat acgcaaattc taaaagtaat
      181 tcatgagaac ccaattctat atctgggttt agttttatac cttttgataa aaaatattca
      241 tttacataaa gtcttgaatt tgctttattt tctagcatta ttaaaggcaa tgtgtttaat
      301 tcttccaatg atattttcct acctttgtat tctatgtaat catttgcaca tacaaatata
      361 tcatgtattt caatacactc aacaatattt agtgtatcat cttctattgg catgtttata
      421 attgctatat ctatattccc atctttgagt aaagatataa gttctctact cgttctattt
      481 attgtagata catgtatatg tggatacttc ttatgaaaaa tttctaaata ttttaacaag
      541 aaaaaacgtg cagctgtatc tcctactcct attttcagag aaccgtattc taatttttta
      601 aatttattta ttctatcttc tcctgatgat attaacttta atgcagtagt gacatgttca
      661 cttagaattt tcccctctgg agttaatttt actcccttat tgtttctata aaataataaa
      721 gtatctaatt gttcttctaa ttgctttatt gactggctta cagctggctg tgacataaac
      781 aattccttag cagcaagaga aaaactctta ttatttacaa ctgcattaaa aaccttataa
      841 agttcaagtt ttatattcat atataattac tccttatgtc aaatattagt attatttatt
      901 ttacttataa ttatatttga attataatat acatggtact aatatgacaa tattaattct
      961 ttaggaggat tttagataat ggatagatta gttggaactg tttcaagagg ggttagagca
     1021 cctattataa gacaaggaga tgacttagtt aaaatagttg ttgattctgt tttaaatgcg
     1081 tcaaaaagtg aaaactttga agttagggac aaagatgtaa ttgccgttac agaagctgtt
     1141 gtagctagag cacagggaaa ttatgctcac gttgacaata ttgcaaaaga tgtaaaagat
     1201 aaatttggag atgatactgt tggggttata ttcccaattt taagtagaaa tcgttttgct
     1261 atatgtttaa aaggaattgc aaaaggatgt agaaaagtcg tattaatgtt aagctaccct
     1321 tctgatgaag ttggaaatca cttaataagt attgatgaac ttgatgataa gggaataaat
     1381 ccttggtcag atgttttaac agaagaaaaa tacagagaac tatttggata taacaagcat
     1441 acatttactg gtgtagatta tgttgattat tataagaatt taatcaagga ttgtggtgca
     1501 gaggtagaaa tagtatttgc aaacaatcca aaaactattc ttaactatac aacaagtatt
     1561 ttaacttgtg atatacatac aagacaaaga acaaagagaa ttttaagaca aaatggagct
     1621 aaaaaagtat attcacttga tgatataatg acagcaagtg tagatggtag tggttacaat
     1681 gaccagtatg gacttttagg ttcaaacaaa gctacagaag aaacagtaaa attattccct
     1741 ataaattgtg atgaagttgt aaacaaaatc caaggtaata taaaagaaat aacagggaaa
     1801 gatgtagaag taatggttta tggagatgga gcttttaaag accctgtagg taaaatatgg
     1861 gaattagctg acccagtagt atctcctgct tatacaaaag ggctagaggg aactccaaac
     1921 gaagtaaaat taaaatactt agcagataat gattttgctg acttatctgg tgatgagtta
     1981 aaagaagcaa tttcaaaata catagttgaa aaagataata aatctgatga tttaacagga
     2041 aacatggttt ctcaaggtac tactccaaga agattaacag accttatagg ttctttagca
     2101 gatttaacat ctggaagtgg agataagggt actcctatta tttacataca aggatatttc
     2161 gataactata caaaatagta tgttttaata agatttaaat tttttggatt atatatatta
     2221 acatgaaaat ttattaaaac ctcttaatag atatgacttt tcaagagtt
//
LOCUS       DAELJO010000357         2227 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.359, whole genome shotgun sequence.
ACCESSION   DAELJO010000357 DAELJO010000000
VERSION     DAELJO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.359"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            100..324
                     /locus_tag="KPB88_003898"
     CDS             100..324
                     /locus_tag="KPB88_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056125.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            377..901
                     /locus_tag="KPB88_003899"
     CDS             377..901
                     /locus_tag="KPB88_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056126.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAEINNNGDKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(1006..1152)
                     /locus_tag="KPB88_003900"
     CDS             complement(1006..1152)
                     /locus_tag="KPB88_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056127.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1291..2217)
                     /locus_tag="KPB88_003901"
     CDS             complement(1291..2217)
                     /locus_tag="KPB88_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0056128.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTNRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
BASE COUNT          945 a          236 c          337 g          709 t
ORIGIN      
        1 ctaaataaat gataagttat aatatattaa tatgaataag cagtatatat acaactaata
       61 ataagaaaaa tatcaattat ataaggagta gataaaaata tgataataaa acttactaaa
      121 gacaatttta attttagatt taaacaatat gaaggtatgt tttttcatgc tgaagtagtt
      181 gaaggtatgg aatccattag atataaaata actaaggata aagaaggtac agaattaaaa
      241 gaatttggaa gtacacatat aaaagataat atgatatatg tacctcaggt agaagcatac
      301 atagatttga acagtataaa ataaagatgt aaaattttaa ttaaaaataa agattatgtt
      361 tgagggggaa tccaatatga gtaagcctat agatataaca ataagaccta taagaagaga
      421 agatgcacaa gcttttaatg aaattagaag aacatacagt gttatgagaa atactttggc
      481 attaatgagt gacagagtcg ataaaactga agctatgtta gcttctttgg gagaaaatga
      541 ttatatgttt gtagctgaaa ttaataataa tggagacaaa gatgtaatag gttttgctgg
      601 gttacatgta aattcttctc cacgattgag acatagcgca gaaataggaa taagtgttgc
      661 agagaattat cagggaagag gagtaggtaa gaagcttatg gaacaacttt tagatatagc
      721 agataattgg attatgttaa ctagagtagg attagaagta attgtggata atgaaaaggg
      781 attaaattta tataaaaaac taggttttga aatagaggga actaaaaaat atgctgtaat
      841 cagagatgga aaatttgaag atgtttatat tatgggaaga tataataaaa atttaattta
      901 aagaagctaa tagagaagta aaatagagtg tctttaataa aaaatttata ttaaagataa
      961 tctattttac ttctctattg tgttaaaaaa cttattttta aatgtttaca aaaacattct
     1021 atatacaaat aaaattatta gcaatatcaa tgctatttta aaagctaaac tagcaacagt
     1081 tttaatgatt tttacagaaa cacctatagc aactatagca aatactattt taaagagtaa
     1141 atccatatcc atatttaagc ctcctattct ataatacata tagatattat tgacaaatat
     1201 ctaggaaaaa attccatcaa tatactacaa taaaatgttt atatttgtaa tttattgaat
     1261 atgttattat ataatacata aaaaaataaa tcaagataag agtaaaatac ttataaaagg
     1321 tatagatata actgaaaata gtgttgtaaa aaacacacat tgtgaagcta gtgttatatt
     1381 ggagtcatat tgattagcca ttatagcagt atttgcagca gcaggcattg aagaaattac
     1441 tactggtata gctaaaatca tcttatcatt tatccatcct tttagaataa aatacacaat
     1501 cacaggaagt accaataatc ttataaaagt gactatatat aattttttgt tgacaaaaca
     1561 atcgagagct gaagaattag caagtaagct tccaatgata atcatggata ttggtgtagt
     1621 tatacttccc aacatctcaa gtggctcgtt tataaattgg ggtaacctaa gtcctgttac
     1681 aaataaaaaa agtccaataa caatagctat tgtagcaggg cttataagtg attttattga
     1741 aaaacctcta tttgtactgc ctttagataa aagataaatt cctaaagtga aagaaaataa
     1801 gttaaaaggc aaattgaata ttgcagtata gaatattgct tcatgaccta aaactgcttc
     1861 aattacagga taacccataa aagcaacatt tgaaaaaaca actatatact gatatattcc
     1921 caaatctttt ttatcattac attttaaaat gtatttcaat aaaaatgcaa ttataattga
     1981 aactatatac ataatcaaag atgtaaataa taacaatagt attttttgta tcattttact
     2041 atcaaagtta atttgcatgg agctaattat cattgatggt aaaaaaactg tcattgtcaa
     2101 agttgaaagc ttagatgtgc agtggtcatc taataagttg aattttctga caaaatatcc
     2161 aactaaaata attataaata aaactgcaac ttggataaaa atattgctga aattcataaa
     2221 atgtccc
//
LOCUS       DAELJO010000358         2192 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.360, whole genome shotgun sequence.
ACCESSION   DAELJO010000358 DAELJO010000000
VERSION     DAELJO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.360"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            129..1733
                     /locus_tag="KPB88_003902"
     CDS             129..1733
                     /locus_tag="KPB88_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0056129.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT          932 a          235 c          348 g          677 t
ORIGIN      
        1 aaacaataat taaaaataaa acttttatat gattaaactc taaagaaaaa gtttataaag
       61 aagataatat aattaagcag aaaataatga aaaaaattta ttaaagtaga caacgtaagg
      121 agaattttat gaaagcaaaa atttattaca atggaaatat catcacaatg gaagattcaa
      181 tatgtggaga tgctattctt ataaaagaca aaataataaa aaagatagga acaaaagaag
      241 aagtatttgc tttgaaaaat aaagatacag aaattataga tttacaaggt aagacattga
      301 tgccatcttt tatagattca catagtcact tgatagcttt tgctactact ctaaagctag
      361 ttcctttaga agatgcaacc agttttaaag acatagtaaa aaaaatacag gatttcaaag
      421 agtcgaataa tataaaaaaa ggagactgga taattggatt ttcatatgat aataattttt
      481 tagaagaaaa taagcatcca gataagagtg tactcgatag tgcatcttca gaaaatccaa
      541 tactaatttc acatgcttct ggtcatatgg gagttgcaaa tacattgggg ttaaaacagc
      601 ttggtgttac caatgaaaca cgtgacccag aaggtggaca tataggtaga gtagaaggca
      661 gtgaagaacc aaatggatat ttagaagaaa atgcattttt taatgtagca tctaaaataa
      721 aacaaccatc atcaaatgag atttttaatt caattgaaaa agcacagaac atatatctaa
      781 gctatggtat tacaacagct caagaaggtt taatggaaga aaatcaattt aacattttaa
      841 aagcaatggc aaatcaaaat aaattaaaaa tggatgttgt aggatatgta aatttgaaaa
      901 aaagtaagtc tgtagcagat aataataggg agtttataaa aaaatatata aatagattta
      961 aaataggagg ttataagatt ttcttagatg gttctccaca aggtaagaca gcatggttga
     1021 gtagaccata tgaaaattca gatgatggtt attgtggata tcctatatac aaagatgaag
     1081 aagtagaaaa gtttatagat atttcactaa aagaaaagat gcaactctta actcattgta
     1141 atggagatgc agcagcagac cagttgatag atgcgtttga gaaagtttta aacttaaaag
     1201 agcaaagtag tgaaaataat ataagaccag ttatgataca tgctcaaaca gtaagagcag
     1261 accagattga tgatatgaaa gtaataaata tgataccttc atactttgta gctcacacat
     1321 attattgggg tgatattcat atcaaaaacc ttggagaaga tagagcgttt aagattagcc
     1381 cacttaaaac aactatagaa aagggattaa tttacactct tcatcaggat acaccagtaa
     1441 tagcacctaa tatgctggaa acagtatggt gtgctgtcaa tagaattaca aaaaaaggta
     1501 tacaaatagg agaaaatgaa aagataagcc cattagatgc tttaaagggt gttactataa
     1561 atgcagcata tcaatatttt gaagaagata aaaaaggttc aattaaagaa gggaaattag
     1621 ctaatttgat aatattagat gaaaacccac ttacaatcga ccctatgaaa ataaaagata
     1681 ttaaagtatt gcagactata agagaagggg aagtattgta ttctttgaag tagatgaaag
     1741 ttagatattt tataatctat aaaaaatagt tttatataat aagttaggta gaattaaata
     1801 agattacagt taaatcaaca attatatttt tattaaattt taattattgt attgaagatt
     1861 tataagtata attttattga ttctaccttt ttaaataact gtcgatttat taaagttatc
     1921 tatgtactat attaaacaat taagtagtta ttaattgaac ttattagttt attaaaaaac
     1981 tatgtatatt actttgcagt aagactttgg aactaaatct ataatagcaa atttaaattt
     2041 attaaataga ttaccttata acatttcata taaaatatat tgtgaatctc agaaaattac
     2101 tatttttctt tgagtatttt ttgtaagcag ttctaaaaga ttcaatttca tacctattac
     2161 ttgaatcatt catcaaaata attctaattt aa
//
LOCUS       DAELJO010000359         2163 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.361, whole genome shotgun sequence.
ACCESSION   DAELJO010000359 DAELJO010000000
VERSION     DAELJO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.361"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            241..507
                     /gene="rpsT"
                     /locus_tag="KPB88_003903"
     CDS             241..507
                     /gene="rpsT"
                     /locus_tag="KPB88_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG0056130.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(694..1737)
                     /gene="holA"
                     /locus_tag="KPB88_003904"
     CDS             complement(694..1737)
                     /gene="holA"
                     /locus_tag="KPB88_003904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG0056131.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1691..>2163)
                     /locus_tag="KPB88_003905"
     CDS             complement(1691..>2163)
                     /locus_tag="KPB88_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0056132.1"
                     /translation="LATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIP
                     LLSIIGNLIIVPFVGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISN
                     LSFAYINIENPKFYIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT          802 a          310 c          206 g          845 t
ORIGIN      
        1 aatatttgtt acaaaaataa aatattaatt tatatatgtc aaaatattta aaacacaaaa
       61 tataccaatt taaaaacaag atattgcaaa ttatttatac acaatttaca gtctaaaaaa
      121 tcaaaataaa aaacattatt aaaatatata aataataatc cttgattttt agatttattt
      181 ctgttaaaat ctaatatgtt gacgatgaat atgataaaaa aatacaggga ggtgaacacg
      241 gtggcaaata taaaatcagc taagaaaaga ataagtgtta tcgaaaagaa aactgcttta
      301 aatagagtta gaaaatctca aataaaaact gcaataagaa gattcgaaga tgctgttgca
      361 gctggaaaca gagaggatgc tgttgctaag ttccaatacg ctcaaaaaag aatataccaa
      421 gtagcttcta aaggaactat acataagaat gctgcagcta gaaaagtagc taaattagct
      481 caaaaattaa acggtatgaa cgcttaatta ccagtttata tttcttatat ataataaccc
      541 ctatgtaggg gttttttatt ttgtaaattt atacaataaa aaataaatag ttagacatat
      601 tatagttgat tgtatatcaa acttaattta gcttacatct actctgaact ataacatatc
      661 ttgctattta ttttttatta atatatagat tttttaaatt gcttctcttt tacagtatct
      721 actaaccaac atttcaactg ctaaagtatc tcttataaga cctgtcttta ttttgtaatc
      781 actttctaat atatagttta atatttccac aattatatca tcagaaaaat ttttagtttg
      841 ctttaatgct tttcctacaa caaattgatg catttttaat ttatcagcta taagttttgt
      901 tgaatatcca tcaagttgta actgtcttac ttgcataatt attttaaatt gtcttgctat
      961 catagaaaat atacctaata cagattcgcc ttcttgtatc atatcattta atattttcat
     1021 tgcattagaa gcattttgtt ctccaatata atcgattaat ttgaatatat cattctcaac
     1081 ttttttttga gataatttat caattacatc attggttaca ttattccctt taccaacaaa
     1141 tgaacttatt ttatttattt cattttctaa atcagataat gtcttttcac tactcttatc
     1201 tctgtatcct tcttgttcaa taaaatacat gatttgagaa ttatctatta taacatcatt
     1261 taaagcaaaa gattttttta tccatttaaa caaatccata tcagaaagct tatcacaatc
     1321 aaaaacaatt ccattgtttc ctattttctt aactaatgat tttcttttgt ctacatctcc
     1381 atatacaaca aaaactatag ttgtggtgtc tggaatattg tctaaatgct ctattaagta
     1441 tttctcatca ctatctgtaa aattcttttt ctttcctttt aatagttcaa agtcttttat
     1501 tattactatc ttcctatcat ccataaatgg aagtgtttct atagagctta taagctgatt
     1561 taagactatt tccttcccat ctattatatc taaattaaaa tcaagcattc cctcgtttag
     1621 actatcttta aatgctttta ttgcattctc aattaaataa tactctcttc cataaaaaag
     1681 atacattttt tcaaagtttt tctcttttac attccttata atatccttgt aattcattct
     1741 cttgctcctt tatctctttt ccttctatgt aaaatatgta aaaaaacact cctatgtaat
     1801 aaataacaac aatataaaat ttaggatttt ctatattaat gtatgcaaaa cttaaattgc
     1861 tgattttttc tagtaaaaaa aatatgcttt ctataaatgt actattaaaa aaagatataa
     1921 ttttagctat tactacactt actttaaata cgattaaact tgcaatactt aaatacatta
     1981 ttactcccac aaatggtact ataattaaat ttcctataat tgaaagcaat ggtatacctt
     2041 taaatgtata ataaattata ggtaaagtta atatatttga tgatattgtc aatgatacca
     2101 tacttaattt tataatacta tttattttat tatagaatat taatatagaa agtgtagcta
     2161 aaa
//
LOCUS       DAELJO010000360         2161 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.362, whole genome shotgun sequence.
ACCESSION   DAELJO010000360 DAELJO010000000
VERSION     DAELJO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.362"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(44..934)
                     /locus_tag="KPB88_003906"
     CDS             complement(44..934)
                     /locus_tag="KPB88_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0056133.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDSGKMMIKAV"
     gene            1083..1943
                     /locus_tag="KPB88_003907"
     CDS             1083..1943
                     /locus_tag="KPB88_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0056134.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          754 a          292 c          269 g          846 t
ORIGIN      
        1 atactaaaac aatttcatca tataatttga aaattattac tatttacaca gcttttatca
       61 tcatcttacc actatcaaat atatattcaa ccatattatt ctcatataaa taagttatat
      121 aagaacgtat tgtacttcca accaacacat attgattagc atcaagcttg acattgtaca
      181 aatcacacat tttttcaata acttcatcaa tcataactgg ctcataacaa acctttttaa
      241 tattatctat tatttcttcc atcttcttct tattaatatt aatcaaactt tttatatctg
      301 tagttttgac agagtgactt ggaacaaaga aatcagcctc taacattctc aatttttcta
      361 gtgtcttaaa ctgtgaatct atatccagta ggaagaaaaa atgatattta gtaattatat
      421 tctctggtgt aagactatct gcaacaaaca aaacattatc tggagttcta acaccaatca
      481 tttcaaaata atgacctgga agagaaatcg cttcaagttc tgtatctaca attttgccat
      541 ttgaaggtat aatataatca acttcacttt cctttgctaa taaaaattta ttcttcagtg
      601 ctttacttgg atagccacca tataaaaatg ctgattctaa tattggattt tctgtaaaat
      661 atccttcaat tttagtcgta gcaatcttgc attttgtttg attcttcaaa tatgcatttc
      721 ctcctatatg gtctgcattt gaatgagtgt ttattattaa tttaggattt aaattatgtt
      781 cttctaataa tcttaaaact tgtctagctg tatccttatt atttccactg tcaattaaaa
      841 ttgcatcctt accatctacg tatacaccta ctattgtagg gagtggaata taatatgtat
      901 ttccttttat atgttctaga gttaatttag ccattttata acctcccttt aaatttactt
      961 ttaattatag cattaatttt ctgaaaattc caatcaaatt gtttttataa tatattttta
     1021 tttcttttat taaaaattgg aaaaactata taatatatat tataatctat atataaggag
     1081 tgatgttatt gaaaaaagat agaataactt tttcagaaat tttaatatta attgtaggct
     1141 gtatattaat ggccatttct cttaacttat tttttaatcc acatgcaatc gctgctggtg
     1201 gtataacagg acttggagtt gttttaaatt cattattcgg tgtagaactt tggattgtca
     1261 atttactttt aaatgttcca ttattcatat ttgcttacaa aatactttca aaaaaagatt
     1321 gttttaaaac ggttttagga ataatttttc ttaccatagc acttaagctt actgcaaata
     1381 tggctacatt agatataaca aatgatatgt atcttgcaat tatatctggt tctattttaa
     1441 tgggagttgg tcaaggactt atttttagaa taaatggctc tacaggtgga actgatttaa
     1501 tggcactcct actaaataaa tattttccta catttagtat acctgtttta atgggtattg
     1561 tagactgcgt tgtagttgtt ttatcaggta tagtaaatag acaggttgaa attgcacttt
     1621 attcaactgt ggctttatac attttagtaa aagttagtga cttgcttatt gaaggattta
     1681 attattccaa atcattcact attatatctg acctttcaaa agatataagt aaaaaaataa
     1741 tggaagattt agatagaggt gcaactattc taaaaggaga aggtgcatat actggagaaa
     1801 ataaaaatgt acttttagta gtagttgaaa agaaagaagt tgtcgaattg aaaaaattag
     1861 taaaaaatgt cgaccccaat gcttttatta taattacaga tatacatgaa gctttaggta
     1921 atggatttaa aaaaatagaa taactaacaa ataaatagca agtataatta acatatacaa
     1981 attcaacttt ttatatgtta tattatactt gctatttata tattataatt gctatttata
     2041 tattataatt gttgttttta agataaaaat taaatgtatc aattattgtt ttatacaagt
     2101 atttccaaat ccgttctcat ttagatagta tgcttagaaa ttttagaatt attgagtcta
     2161 a
//
LOCUS       DAELJO010000361         2137 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.363, whole genome shotgun sequence.
ACCESSION   DAELJO010000361 DAELJO010000000
VERSION     DAELJO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.363"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            106..963
                     /locus_tag="KPB88_003908"
     CDS             106..963
                     /locus_tag="KPB88_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0056135.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1060..1929
                     /locus_tag="KPB88_003909"
     CDS             1060..1929
                     /locus_tag="KPB88_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0056136.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          891 a          208 c          376 g          662 t
ORIGIN      
        1 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
       61 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
      121 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
      181 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
      241 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
      301 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
      361 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
      421 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
      481 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
      541 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
      601 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
      661 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
      721 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
      781 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
      841 aatcatgcac aatttggaga ctatggaaaa caaaagggag ataatgatgc tattataagt
      901 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
      961 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
     1021 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
     1081 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
     1141 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
     1201 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
     1261 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
     1321 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
     1381 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
     1441 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
     1501 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
     1561 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
     1621 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
     1681 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
     1741 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
     1801 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
     1861 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
     1921 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
     1981 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat tttttattaa
     2041 aaaacaaaat ggtatattca tatataatat atcaataata atgaaaatgt gaactagttt
     2101 aatagaattt tacctattat aataaaaaca ggaattt
//
LOCUS       DAELJO010000362         2131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.364, whole genome shotgun sequence.
ACCESSION   DAELJO010000362 DAELJO010000000
VERSION     DAELJO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.364"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..133)
                     /locus_tag="KPB88_003910"
     CDS             complement(<1..133)
                     /locus_tag="KPB88_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0056137.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     "
     gene            complement(358..534)
                     /locus_tag="KPB88_003911"
     CDS             complement(358..534)
                     /locus_tag="KPB88_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056138.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(588..677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(857..1057)
                     /locus_tag="KPB88_003912"
     CDS             complement(857..1057)
                     /locus_tag="KPB88_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056139.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1059..1409)
                     /locus_tag="KPB88_003913"
     CDS             complement(1059..1409)
                     /locus_tag="KPB88_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG0056140.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
BASE COUNT          838 a          292 c          237 g          764 t
ORIGIN      
        1 gaccttcttc taatgattta aatcctgaag tttgtatagc tgagaaatga gcaaatacgt
       61 catctccact ttccattgat ataaatccaa atcctttttc attattaaac cattttacta
      121 ttccattatt cattaaaaat actcctatta acctatatca agatatactt gatttacttg
      181 tgtattacca caaatactat tgtatcatag attaacataa atgtctattt aattataatt
      241 tttagttaac atttttattt attcttaata tgaaaataaa tttaaacatt ttatattacc
      301 aatatattaa tgtaaatgta gataaaacta aaaagggttt tctagaaaaa aatctgtcta
      361 attatactta cttaccaata ccatcacttg atgcaagcaa gattccacaa ctttttctcc
      421 gtactctaat ataaaatcat ctaactgtgt atctataaaa tcacaataat catatgaact
      481 accaatataa gcagttaaca tatgtgtaca tttatccatt ttatctctaa acataatgta
      541 tttatccccc taaaaaactt tcatgaaaaa caaaaagaaa atatcttttt ggcaactggc
      601 tgacataact tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag
      661 ttttgccgat ttagttttat tctacagatt aataatacaa tagaattagt tattattcaa
      721 catatctagc tgaaaaatta ataaatttat atatgaacgt taaattatct gaaaaaataa
      781 aaagcccctc tgtcaggagt gacttaaaaa ttgaatttaa gtttgtattt aaaacacatt
      841 tttaataatg caaaaactaa tcaaaaataa acaattcatc aataggtgta tctactattt
      901 ttgatatatc tattgctaat ttcaaagaag gattatactt tcctgcttct aaacgcataa
      961 tggtttctct tctaactcct actttttctg ctaattgttc ttgagttaat tcttttaatt
     1021 gtctatattt ttttaggtta caaatgaatt tgggcatatt atttttcctc taaaatataa
     1081 agttttatag cataaacatt taaagtgatt gcatatgtaa ttgatagtaa aacacacatt
     1141 aattctatat tcctagacac tatagtaata caccataaaa tgaataattc tataatagca
     1201 agctgtcctg taaatgccat agctgctttt tcatcttgta catacctttc atctgcttta
     1261 tcaccattta tttttgctat aataaagttt gagaaaaaag caaaaaatcc aatatatgta
     1321 aggtcagcaa cattgccaga tgaaaaatat ctaagactta aaaatccaag gaaaccacaa
     1381 aaacccataa aaaatttttt ctgaatcatt ttttatcctc cttagtaatt ttaaaagaga
     1441 tatatttata tctttatgat acaaatatat ctcttttata ttcttttgtc aatagaaaat
     1501 cagtattata caatatttgt actgcaccat aattgttaga gcaaaaatct actgcttgag
     1561 atgtttatgt atgactaaat atgattttaa tttcaaatta aaagtagcta aattttattt
     1621 gaatagagaa ggaagagata ttttaatagt aaaacaatat ggagtttata accattctca
     1681 agttgaatga agggttaata tattaggaga gtgtgcacta aaaataaaaa aaattttcta
     1741 tactatatgg ttcaagttag atgttataaa ctatatgatg ataactaaat atagtgtaca
     1801 ataaatcgct aagtattttg gaataaacaa tagcacagtt atagcaacat gaaaataaaa
     1861 atttttacat agtggtgttg atgccttttc taaaatgaaa ggaagaccat aatgaataaa
     1921 aacaaaaaca ctaaagaaaa aataaattaa caagagaaca agaattagaa tatgaaaata
     1981 aatttttaaa agatgagctt gagtttttaa aaaagatgtg tgcctcaggg aagccacatt
     2041 aaaaaacagt ggtatcatta ttaatcaaaa gaaagttaga tgtattatca aaaaattaga
     2101 acttaaatgc atagcatttt ttcataaatc a
//
LOCUS       DAELJO010000363         2105 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.365, whole genome shotgun sequence.
ACCESSION   DAELJO010000363 DAELJO010000000
VERSION     DAELJO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.365"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..166
                     /locus_tag="KPB88_003914"
     rRNA            <1..166
                     /locus_tag="KPB88_003914"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            299..415
                     /gene="rrf"
                     /locus_tag="KPB88_003915"
     rRNA            299..415
                     /gene="rrf"
                     /locus_tag="KPB88_003915"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            422..496
                     /locus_tag="KPB88_003916"
     tRNA            422..496
                     /locus_tag="KPB88_003916"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Asn,seq:gtt)
     gene            501..575
                     /locus_tag="KPB88_003917"
     tRNA            501..575
                     /locus_tag="KPB88_003917"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:534..536,aa:Glu,seq:ttc)
     gene            581..656
                     /locus_tag="KPB88_003918"
     tRNA            581..656
                     /locus_tag="KPB88_003918"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Val,seq:tac)
     gene            662..738
                     /locus_tag="KPB88_003919"
     tRNA            662..738
                     /locus_tag="KPB88_003919"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Asp,seq:gtc)
     gene            749..823
                     /locus_tag="KPB88_003920"
     tRNA            749..823
                     /locus_tag="KPB88_003920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:781..783,aa:Thr,seq:tgt)
     gene            838..922
                     /locus_tag="KPB88_003921"
     tRNA            838..922
                     /locus_tag="KPB88_003921"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Tyr,seq:gta)
     gene            932..1005
                     /locus_tag="KPB88_003922"
     tRNA            932..1005
                     /locus_tag="KPB88_003922"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:964..966,aa:Gly,seq:tcc)
     gene            1016..1092
                     /locus_tag="KPB88_003923"
     tRNA            1016..1092
                     /locus_tag="KPB88_003923"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:Arg,seq:tct)
     gene            1102..1177
                     /locus_tag="KPB88_003924"
     tRNA            1102..1177
                     /locus_tag="KPB88_003924"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Gln,seq:ttg)
     gene            1189..1264
                     /locus_tag="KPB88_003925"
     tRNA            1189..1264
                     /locus_tag="KPB88_003925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Lys,seq:ttt)
     gene            1267..1355
                     /locus_tag="KPB88_003926"
     tRNA            1267..1355
                     /locus_tag="KPB88_003926"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Ser,seq:tga)
     gene            1373..1463
                     /locus_tag="KPB88_003927"
     tRNA            1373..1463
                     /locus_tag="KPB88_003927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1407..1409,aa:Ser,seq:gct)
     gene            1472..1548
                     /locus_tag="KPB88_003928"
     tRNA            1472..1548
                     /locus_tag="KPB88_003928"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1506..1508,aa:Pro,seq:tgg)
     gene            1634..1709
                     /locus_tag="KPB88_003929"
     tRNA            1634..1709
                     /locus_tag="KPB88_003929"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1667..1669,aa:Trp,seq:cca)
     gene            1770..1846
                     /locus_tag="KPB88_003930"
     tRNA            1770..1846
                     /locus_tag="KPB88_003930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1804..1806,aa:Pro,seq:tgg)
     gene            1853..1929
                     /locus_tag="KPB88_003931"
     tRNA            1853..1929
                     /locus_tag="KPB88_003931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1887..1889,aa:Ile,seq:gat)
     gene            1933..2008
                     /locus_tag="KPB88_003932"
     tRNA            1933..2008
                     /locus_tag="KPB88_003932"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1966..1968,aa:Phe,seq:gaa)
     gene            2016..2092
                     /locus_tag="KPB88_003933"
     tRNA            2016..2092
                     /locus_tag="KPB88_003933"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2050..2052,aa:Met,seq:cat)
BASE COUNT          543 a          476 c          567 g          519 t
ORIGIN      
        1 cggaatggat aagcgctgaa agcatctaag cgcgaagcca acttcaagat aagatttccc
       61 accgcaaggg taagacccca gaaagactat ctggttgata ggtcgaaggt gtaagtgcag
      121 caatgtattt agcttatcga tactaatagg tcgaggactt gaccaatatt atttgatgtc
      181 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
      241 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      301 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      361 tagcgctgat ggtacttggt gggaaactac ctgggagagt aggacatagc cacgtgaatg
      421 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      481 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      541 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      601 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      661 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      721 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      781 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      841 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      901 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      961 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
     1021 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
     1081 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
     1141 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1201 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1261 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1321 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1381 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1441 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1501 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1561 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1621 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1681 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1741 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1801 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1861 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1921 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1981 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     2041 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     2101 tatca
//
LOCUS       DAELJO010000364         2073 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.366, whole genome shotgun sequence.
ACCESSION   DAELJO010000364 DAELJO010000000
VERSION     DAELJO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.366"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..1833)
                     /locus_tag="KPB88_003934"
     CDS             complement(<1..1833)
                     /locus_tag="KPB88_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0056141.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKIL"
BASE COUNT          717 a          352 c          166 g          838 t
ORIGIN      
        1 caatattttt tcaaattcta atattggcat tggtttataa aatacaaatc cctgaactaa
       61 atcacaccca atatccctaa gaaattcaac ttgttccttg cattcaatgc cttcagcaac
      121 agttgtgata ttaagttctt ttaacatatg tacaatattt tttattacta ttttctcttt
      181 atctttatct aaactagcct tgaaaaacat agagtcaagc tttaaaacat caaatggtaa
      241 atcttttaaa acacttaaag acgaataacc ttttccaaaa tcatctaaag aacaagtaaa
      301 tccattttca tgcaaatcat gtataatttc caaaagatgt ttttgattct caaaagcaac
      361 actctctgta aattcaattt ctatagtatt tttaggtatc ctgtatttat tttgtatatt
      421 ggaataagtt tctacaaatt tagtgttata aaattgtatt cttgaaacat taactgatat
      481 ctgaacaaaa ggtttatttt catctaacct ctttctaatc catttacaaa cctcctcaaa
      541 aacatactta tctactaagg caataaaaaa attcttctca agtactggaa taaatattgc
      601 tggcgatata atacctttat ctggagtaag ccaacgaact aaagcttctg cacaatttat
      661 tttttgacta tgtaaattaa ctttaggctg taaataaact ataaattctc ttttttcaat
      721 agcttcatgt attctactct tgatagtatt ttcttcaatc atttttttac gaatactgtc
      781 attataaaaa gcataattag tacctggttt attcttaaca gtcttttgtg caaaatttgc
      841 tctatttatt aaaccatcta tacttaattt ttcatttaca tcttcaatac aacaaattcc
      901 actaacaaca gtaatactat gtttattttt aatttcattt gttatattaa ttagttcatt
      961 gtctacatct cgttgtctta ccactaaatc ttccttatct acatagcatc ttaacgcaac
     1021 aaatctatct gcaatttctc ttgcaaatat ttcatatttc cctatgtcat tcatcataag
     1081 attagcatat cttttcagta tcatgtcacc ataatcatat ccaaatatat cattaatgta
     1141 tttaaagttt tcaaaatcaa tatagaatac agcaaatttc ttatccttat gtatacaaat
     1201 attttgattt gcatccaatt taaatttttc tatagtattt actcctgtta gttcatcttt
     1261 atatgcaata ttttttaaat ctacatttaa tttttccagt ctaaccattt ttttactata
     1321 ttgtaaaatc aatatgatac atgctaatat acctataata gatataatta ttattaaagt
     1381 cattaagtaa ttactttttt ctttagaaaa gttttctatt ttaaacactg tatcatttgc
     1441 aagagaaaaa aactcttcac ttatacttaa taactggtta ttctgttctt tcattcttac
     1501 ttttttaatt tcaatattta aatcttccca ctttttttct aataaaagta agtcctcatt
     1561 gtattcatta caatcagtga ttactaaacc ataatctcca tgccctgtaa tcaactcctg
     1621 taggatttca tcaatatatt ctattaattc atcatttggt tgatgattca tctctagttt
     1681 agttagacgt tggctagctc cacgaacaat cccaacataa ttaatcaatt ttccataatc
     1741 tccaacatta tgaattaatt taatagaaat aaaacttata aaaatcaaca aaataaataa
     1801 aactaagata attctcctca aaaatctctt cataaaacac ctttatcccc tatattgttt
     1861 tttatgttta ttgaatacct tcaaactatt ttttctaata atatacatgc gtttacaata
     1921 aattttgcta tactattaga atatataaac cttgttttac atagttcatc tttagagtcc
     1981 tctatatata caaaaatttt tttctaatat ataagttctt atagattatt actcttaatg
     2041 taatttataa actttttaat tacaaaccac cta
//
LOCUS       DAELJO010000365         2060 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.367, whole genome shotgun sequence.
ACCESSION   DAELJO010000365 DAELJO010000000
VERSION     DAELJO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.367"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            225..839
                     /locus_tag="KPB88_003935"
     CDS             225..839
                     /locus_tag="KPB88_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0056142.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            857..1219
                     /locus_tag="KPB88_003936"
     CDS             857..1219
                     /locus_tag="KPB88_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056143.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1326..1823
                     /locus_tag="KPB88_003937"
     CDS             1326..1823
                     /locus_tag="KPB88_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG0056144.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          783 a          227 c          337 g          713 t
ORIGIN      
        1 tataactcaa ttaatttttt aaatacaact ttataatata aaacttataa taaaagtaaa
       61 tttatatgtt aaatgaggtt gattttttat tattttttat ttatgctagt actaaagact
      121 tgcaaacgtt ttttgacaag tctttatttt gtctaaatta aacaaacttt tttacaaata
      181 aatacataaa atttaaacta atctatttat tggaggagat aaaattggaa gaagtaattg
      241 caaaaattat tactatagaa aatggattca aagaaattga aaacattgct aaaaaaattg
      301 taaaaaataa tgactcaaaa aagtgttatt atctatcaat ctacttatat aggtcagagt
      361 attatcaagt aagagaattg gctgtgttta tacttggttt tatatcagta actatttctg
      421 aagctttgtt atttttaaaa gataatgtga gtaaagatga aaactggaga gtacaagaaa
      481 tactagctaa atcatttgat tattattgca gtgaagtagg atatgaaaag gcacttcctg
      541 ttataaaaga atggcttaat tctgatagtc caaacataaa aagggctgtg acagaaggtc
      601 ttcgaatttg gactaataga gactatttta aatctaatcc tgacgttgct atatcactac
      661 tttctagttt aaaagaagat gatagcgaat acttgagaaa gtctgtaggt aatgctctta
      721 gagatataag taaaaaatat ccagatttag taaaaaaaga attagattca tgggatatat
      781 caaataaaaa agttgagttt gtatataagc ttgcttcaaa atgtatcttg gcaaagtaaa
      841 atggaggtga acttatttga aacttcgtga tgagtatact tgcccattag aactaacaca
      901 tgatatgata aaaggcaaat ggaaatctat tatattatgg agattacgca taggaccaac
      961 ttccttatca aaacttgaaa aggaaatcaa tggtattaat caaaagatgt tacttgaaca
     1021 acttaaagaa ttaattgaat ttggtcttgt agataaaaag acatatgaag gttacccact
     1081 taaagttgaa tactcactta cacctattcg agggaaacaa atattggatg ctttggctat
     1141 aatgcaagat ataggaatta gctttatgtt ggataatggt atggaagata ttcttaagga
     1201 aaaaggtgtt ttaaagtaat atagataccc acaaaaaagt aagtaattta ccattatttt
     1261 caactggtat ataattaaat tatagatata ttctattaat ttattataat aggaggtaaa
     1321 ttattatgaa agtaactagt acaggtatag taaatggtgt tatagaagat aaatacggta
     1381 aaagaggaac tcagttcaat gaaggtggta tgcctaccta ttcactacct ctaaagtttg
     1441 aagatgctcc aaagaataca gtatcctttg ctgtattttt agaagataaa gactctgttc
     1501 ctgtatgtgg gtttgcatgg atacattggt tagctgtaaa tataacaaaa gatgagttaa
     1561 atgaaaatga aagtgtaaca gctactgatt ttgtacaagg gactactagt tggcatggta
     1621 aactaggagg tcttgacaga ttagaggcca gtgtatatgg tggaatggct cctccagaca
     1681 gacctcatgt ctatgaaata catgtatttg cacttgatac tatccttgac cttgaaaaag
     1741 gattctatat gaatgaattt ttcaaagcta ttgaagggca tgttttagat acatttacac
     1801 taaaaggaag ttattctaat taattaggtt gactatagct aatcattaat taaatgctac
     1861 atttaattga tgattagctt aattgttatt agaatatttt ctgcacttta tcttaatata
     1921 tattttagtt gactggtatt ttaatttcta ttagattatt atcaatttat ataaattgat
     1981 tttagcttaa aacttactta tctatttcag tttctattag aattattatt agactcatta
     2041 aaataaaagc actaatataa
//
LOCUS       DAELJO010000366         2030 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.368, whole genome shotgun sequence.
ACCESSION   DAELJO010000366 DAELJO010000000
VERSION     DAELJO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.368"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..329
                     /locus_tag="KPB88_003938"
     CDS             <1..329
                     /locus_tag="KPB88_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056145.1"
                     /translation="DKILTSREITEAMEQKYEWKDTTTFTVLKRLQRREFLETEKIDK
                     RTHYNVLVKEKQYQRFETREFIKNIHKNSIKSLISALHKDDEKVDEKKLDELEEYFNN
                     LEENEK"
     gene            complement(520..1365)
                     /locus_tag="KPB88_003939"
                     /pseudo
     CDS             complement(520..1365)
                     /locus_tag="KPB88_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
     gene            complement(1556..1969)
                     /locus_tag="KPB88_003940"
     CDS             complement(1556..1969)
                     /locus_tag="KPB88_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056146.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          775 a          304 c          254 g          697 t
ORIGIN      
        1 cagacaagat attaacatca agagaaatca ctgaggctat ggagcaaaaa tatgaatgga
       61 aagatacaac tacatttaca gtattaaaaa gattgcaaag aagagagttt ttagaaacag
      121 agaaaataga taagcgtaca cactataatg tattagtaaa agaaaaacaa tatcagagat
      181 ttgaaacaag agaatttata aaaaatatac ataaaaattc aattaaaagt ttgatttcag
      241 cattacataa agatgatgag aaagtagatg aaaaaaaatt agatgaacta gaagagtatt
      301 tcaataattt agaagaaaac gagaaataaa attttacaaa gaaaaaatgg gtggcaaatt
      361 gtttttgcca cccatttgcc accaaactgg tttaataaaa taaaatacaa ataaagaagc
      421 ataaagaaaa taaaataaag aaatcgtttt agatgaattg aaaatagatg tattcataaa
      481 aacataaata aatataaata agcataaaga aaatatgaga atttcaagag taaattcagc
      541 tctttcaggt accacatttg atgaagatgg atagaaattc atctttccta cagtagcaac
      601 caaatcagta gtcgttttag ctactttttc gatagctaat acacattcac aggcacaaca
      661 tccagcatca agtctcatat tcattggaag tgtaccagca tgtccagact taccatttac
      721 tgatatctta aaacttttaa atcctgtaat acttgataca attccagcag gaagagattc
      781 attttctaaa acaggaccct gctcgatatg aaattctaaa tatgcatcta catcttttct
      841 aggatgcaaa tttgttaagt tttttgtatt aaatccaaac tttgttacag cttcttcaag
      901 tgtaattcca ttttcatcaa cagatttaag aagttcctct ttaaaagtac cagcaaaaac
      961 tttacttccc attaaagaag tttcaaatct agttccttcc tcttcagcga aagcaattag
     1021 cttaatattg aaaggataag acctattatt gtgttttgct tcttcaagac caactagagg
     1081 cactattata cctaacatac catcaaatat acctccacac tttacactat cttgatgaga
     1141 tgcaactact aatgtgggag catttggaaa gcttgattta tatgtaccta taacgtttcc
     1201 aatgtcatca atttcaatat caagatttaa accttccatc catgaactaa ttaaatcaat
     1261 agatttttta tgttcttgag tcaagataag tcttgtaaga ccatttgttg ttgaacttat
     1321 ttttcctaat gtttgtagat aatccataca tttttttcct aacatattta cctcctaaaa
     1381 atataatttt tatttaagcc aagattagct tttatactta taatttttga actttcaaat
     1441 aattttgtat gtatagtaaa atactatttt agtattaata atgatataaa aacataaagt
     1501 ctgtagacga agaacagaag ttttaagtta aaaatatgaa atatgatttt tattattata
     1561 gtatttttct ataataatat ctctttccag ttagaggata attttcaagt gaaaatactt
     1621 tatggtatcc aaatttctca taaaatttag gtgcttgaaa actgaatgta tctaaaaaaa
     1681 catatttaca acctcgttct ttagcaattt tttcagcctt atataaaagt tgacttccag
     1741 tacctttata tctaagcttc tcactaaccc ataagtattt tatagaaagc caattaccat
     1801 gagtaacacc tataaaacct gcaacaattt tacctgcttt atctcgaata tatattccta
     1861 aatccactgg gtttttatct tcaagtctag caagattata ttttaataat ccttgaaaaa
     1921 tagtatcttc atcttgtttt tctatttttt ctgtaatttc taattccata tttgaacttt
     1981 ctccttgtat aatcttttgt atattggtat tttttaataa aatcagtaga
//
LOCUS       DAELJO010000367         1959 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.369, whole genome shotgun sequence.
ACCESSION   DAELJO010000367 DAELJO010000000
VERSION     DAELJO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.369"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..138
                     /locus_tag="KPB88_003941"
     CDS             <1..138
                     /locus_tag="KPB88_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0056147.1"
                     /translation="KNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQN
                     K"
     gene            265..1167
                     /locus_tag="KPB88_003942"
     CDS             265..1167
                     /locus_tag="KPB88_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0056148.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTIYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
BASE COUNT          662 a          191 c          392 g          714 t
ORIGIN      
        1 aaaaacgagg aagtgaagga cttaaatata agagaatgga tttgtcctag ttgtaatgaa
       61 actcacgata gagatataaa tgcaagtata aatatattaa aagaaggatt aagactaata
      121 acaattcaaa ataaataaca tatgtaagaa ccgtaggaac tacggggata gcctgtggag
      181 aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct gtgactttag
      241 tcatgggagg ttcagattgg aaatgtgttg ccacaagatt attatggatt aatagtgatt
      301 ttaggtgtta ttcttggagt tatgggggca ttttacaact ggtttactat atatgtgcag
      361 tctttataca agaagattcc taaaattggt acgtttggaa aactgttgat tccatttata
      421 atggctggta ttcttgggct tgttatgcct gatgttctgg ggagtggaca caatttagtt
      481 gaagaactta caagtcatga atatattttt ggaatggttg ttttgatttt tgttatacgt
      541 tttatatttt cggctgttag ttttgggtca ggtgcaccag ggggaatatt ttttccattg
      601 cttgttttag gagcatttat aggtggaata tttggtatgg taggtgttaa agtttttgga
      661 atgaatcccg attatgtaaa taactttgtg cttttggcta tggcaggata ttttacagct
      721 attgtgcgtg cccctttgac tggtatcata ttaatttttg aaatgacagg ttctgttagt
      781 caaatgcttt cactatcagt aatttcaatt gtggcatata ttgtggctac acttatgaag
      841 tctaaaccta tttatgaaag tcttcttgag aggttacttg aaaaaaatgg agaacctgta
      901 cataaagaga ggggcgaaaa gatactagac cagttctctg ttatgtttgg ctctcctgtt
      961 gcgaacaagg caattcagga tgtacagtgg cctgaaaatt gtcttttggt agcaattcaa
     1021 agaggtggag aagaaattat tcctagaggt aagacaattc ttagaccaag tgatgtaatt
     1081 gtgactatga cagatgagag agatagtggt gctgtgtatg ataagatgga aaatttgtgt
     1141 aaagaacata cgtcacatat tttttaggga taatgatgaa tgtttaataa tgatagaata
     1201 tttaatgaag atttgataat taaaaaattg gtaatttgaa agagagttaa ggaattaata
     1261 taagaaaatg aaaatgtgat atcttagatg taaaagagtt tgaagaaaat aattgtatat
     1321 atttgttaat gagattatat gaactaatgg tatatttata ctgtgctaaa agttgcacta
     1381 ttgtatgagg aatgggaata aagagtttga aaaaataggt agaagatttt tcagatattc
     1441 ttattgggat attttaatta atatatttta attgcgatat tttgggaaga atagaattta
     1501 tttataaatg aaaataatta aaaggggtta aggttgtatt agcccctttt gcttatagag
     1561 tttggaaatg gtatcttgat agtagttgtt tcatgcttat tatattttat tacatatttt
     1621 aaaattgacc ttattactcc taaatcattg ttatatcaac ttttaaaaca ctttcaagac
     1681 aatcgcctat tgaaaacaaa gttaattaaa aacctacata tcagtttagt attcgtgtag
     1741 atgattatat aaatataaaa gtataagaaa ggtgagtatc tgtggttagt tcatttttaa
     1801 atgatatctt tagaacaatt ttagtaagtt gtatttttat agttatttta ttagtcttta
     1861 gaataacttt atttaagact ttcagtaaaa aatttaatta ctatatatgg cttattgtga
     1921 ttattaagct atcattacca tttatgaatt atactttta
//
LOCUS       DAELJO010000368         1958 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.370, whole genome shotgun sequence.
ACCESSION   DAELJO010000368 DAELJO010000000
VERSION     DAELJO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.370"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(43..955)
                     /locus_tag="KPB88_003943"
                     /pseudo
     CDS             complement(43..955)
                     /locus_tag="KPB88_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            1211..1567
                     /locus_tag="KPB88_003944"
     CDS             1211..1567
                     /locus_tag="KPB88_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056149.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1682..1858)
                     /locus_tag="KPB88_003945"
     CDS             complement(1682..1858)
                     /locus_tag="KPB88_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056150.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
BASE COUNT          693 a          267 c          263 g          735 t
ORIGIN      
        1 agtattaaaa agttcaaaaa tttacttaga aggaatctat aattagattt ctatgtttgt
       61 tgagaatgca attacagctt ttccaattat ttttacttca actggcttat tatctaaaat
      121 aattactttt actaacatct tgcctttgcg tttattgctt ctccttgaac tgcttcaaat
      181 ataaactctg tgttgggtgt aggtattact ttataatgta caaggtacgc acctaagggt
      241 ccattagcat ttcctgtaac agggtcttct gaaataccaa ttgcaggagc aaacattctt
      301 ccatgaactt gtagagtttc tttattatgt aatgtaaaaa tataatgtcc attacatttt
      361 atttcttgac ttattttcga taattcatat aaatttggag ataaactatt taatgttgat
      421 aattccttta ttccaatcat aacttttgaa tgtcctgtag atgatatggc aataggacaa
      481 tctgtacgaa tatcttcctt taaaatacca aatgcattaa gtaatctttt ttgataggtt
      541 tgttgtaatg gtggagatac ttcaattttt ccttgtgtca taattatttc ataatcatta
      601 tcttttttta taatatcaac tggtaatatt ccagcaccag ttttttgaaa tactgttttg
      661 ttattgaatt ggttttctac tgctctaaca taatgggcag caatagtagc atgaccacat
      721 ataggaacct cattagttag tgtaaaaaat ctgatatgta catcataatt ttcttgatga
      781 tttttagcca agttattgaa aataaaagca gtttctgaat tatttaattc tcttgttatt
      841 ttttgcattt gtgattcaga taagccatca gcatttgtaa ttacaccagc cggattacct
      901 gtaaattttt cttttgtaat gagtcaactt gataaagacg aaatttttta ttcatattat
      961 ataatccttt ctaaacatta gattagttta tctacataga aaacttgaga taaattatta
     1021 tttattatta tctgtttaaa taaaattaat aaaataacat atgtctatat taaagtttaa
     1081 acatatacat ctaaaaaagt agtattatgt aaggctctat actacaatca agattatatt
     1141 acgaattagt acgctaaaca agaatgcact ttttagttag atacttacta ttttgaaaga
     1201 gaggattgag atggatatag ataataattt tgaagaattt ccaaatattg aagaaagaca
     1261 aaaagatttt aattgttcaa ttggttttac catgactgtg ataggaagta aatggagggc
     1321 aattatttta tggcatattt taaaacaaga gccaatacgt tacagtcaat taaaaaaaga
     1381 agttgttcat ataagtcata agatactgtc acaagaatta aagtatcttg aaagagatgg
     1441 acttattaag aggatttctt atccgactat tcctccaaaa gttgaatatt tgtctactaa
     1501 acgtggtaaa tcattagaat ctatattatt agaattatgt aattggggta aacagtatat
     1561 gagataaact caactgttta ctttatgaaa aatatataat gaagcttatt ttaattagtt
     1621 ctttaatgaa ttataaaacg tagataaaac taaaaagggt tttctagaaa ataaaatttc
     1681 tttaattata tctgcttacc aataccatca cttgatgcaa acaagattct accacattct
     1741 ctccatattc tagtataaaa tcatttaatt gtgtatctat aaaattgcag taatcgcgtg
     1801 aactgcatat atatgcagtt agtatatgtg tacatctatc cattttatcc ataaacatat
     1861 atcatatatc cccctaaaaa acattcataa aaacaaaaag aaaatatctc tttggcaact
     1921 ggctgacata actcatttca taagtcttag tccctata
//
LOCUS       DAELJO010000369         1951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.371, whole genome shotgun sequence.
ACCESSION   DAELJO010000369 DAELJO010000000
VERSION     DAELJO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.371"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(19..468)
                     /locus_tag="KPB88_003946"
     CDS             complement(19..468)
                     /locus_tag="KPB88_003946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056151.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKEDCEWQRVS
                     NIGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLG"
     gene            complement(586..951)
                     /locus_tag="KPB88_003947"
     CDS             complement(586..951)
                     /locus_tag="KPB88_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056152.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVHGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            complement(965..1222)
                     /locus_tag="KPB88_003948"
     CDS             complement(965..1222)
                     /locus_tag="KPB88_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056153.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKEYVYAMFGEINNFKEIGKYEDE
                     KRAMQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            complement(1240..1461)
                     /locus_tag="KPB88_003949"
     CDS             complement(1240..1461)
                     /locus_tag="KPB88_003949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056154.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQHKKYKELWIDDIEGVEFTVE
                     KTFDDGCICYPNEFWLDFGVISEWCVEVK"
     gene            complement(1451..1627)
                     /locus_tag="KPB88_003950"
     CDS             complement(1451..1627)
                     /locus_tag="KPB88_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056155.1"
                     /translation="MSRTAICSVCGEFLVDSYGDEFTNAKVIDLYGREFVIVICKGCL
                     EELLRKCEEQQYEI"
     gene            complement(1697..>1951)
                     /locus_tag="KPB88_003951"
     CDS             complement(1697..>1951)
                     /locus_tag="KPB88_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0056156.1"
                     /translation="EQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKV
                     NVNALELLSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
BASE COUNT          597 a          424 c          144 g          786 t
ORIGIN      
        1 cctactataa gtattaaact agccaagatg ttcattttct aacatctcct tacgttctaa
       61 gaactctttt ttaatttctt ccaaattctc gcattcatta ccaactacta catatttttt
      121 gctattcatt atagttgctt tacttgtaat ttcctcccat ttaccttcaa attgctttaa
      181 ataatgccat tcaacatata attcaagagc ataattttct tgtcttacaa ttccatattc
      241 atatttattt ttagtattat agggttcttt caatatatct ccctcataaa tttcttcatt
      301 atttccagac caacatttag agaatactcc aatattagat actctttgcc attcacaatc
      361 ttcttttagc ataaataaac aatctactgc atcactccac tttattgttt cagaataaat
      421 ccacttctca ttctcaaaat cataacctct atacttaatt aaactcactt ttaatcatct
      481 cctcatattc ttctctagcc ttatctatag caataaatat gtcctctcca ttgtcataca
      541 actcttttgc tcttttaatt gtgtattcag tccttgaaac ttccattatt cctccttaat
      601 atattcagct ttccagccac ttcttgtttt agttttcttt ttaattgttt ggtaaactgc
      661 ctgactctgt agtcttaaaa aacacgctgc actatctata gaatcaaata ttttttcttc
      721 accagttttg gcattaatca actttacctt tgaacctttc tttttctttt ttctattttt
      781 atcaacatta aactctatta gcattttttc agaagttgga aatacaagct ctccattttt
      841 tcttactcca tgaacacaac aatatagtgc taagtagttt ctaagagtca aatcatcttc
      901 aaatatgtta tctgcaacag aaccattaaa atatttttca acctttaaca tttcagtaac
      961 ctccctattt aactggcatt tgaaatattc tatttctata acttctaacc ttataactgt
     1021 ctatagaatc cgtcctagtc ccaccttcaa taaatctttg tatattatct aatacctgca
     1081 tagctctttt ttcatcctca tattttccta tttccttaaa gttgtttatt tctccaaaca
     1141 tggcatatac atattcttta tcaacattta tccaatcagc ttttactaaa tctgttttat
     1201 cttgacttct aattattatc atttctaata ccccctacct tatttaactt caacacacca
     1261 ttccgaaatt acaccaaaat caagccaaaa ctcatttgga taacaaatac aaccatcatc
     1321 aaaagttttt tcaacagtaa attcaacacc ttcaatatca tcaatccaaa gttctttata
     1381 ttttttatgt tgttctacac cattaatttc cataaatttc tctttatcaa acttataagt
     1441 tttacctatc tcaaatctca tattgttgtt cctcacattt tcttaataat tcttctaagc
     1501 aacccttgca tataacaatt acaaattctc taccatacaa atctataact ttagcatttg
     1561 taaattcatc accataactg tcaactaaaa actccccaca aacactacat atagctgttc
     1621 tactcatttt tatccctcca tttttaactt ttaggaagta atattgtata attactccct
     1681 agactattta acttaattaa aaaggtatat cgtcatcatc tattgcttga aaaccttgtg
     1741 ggtctaatcc tggtggtaca tattcttgtt tagcattatt atcattttta ctagaaagta
     1801 gttctaaagc atttacatta accttagtaa tagatttcca gcaaccattt tcatctttgt
     1861 aattatatat atttaactct ccaacagcat atataggctt acctttaaca agatattgca
     1921 ctaaattctc tacatgtttt cctaattgct c
//
LOCUS       DAELJO010000370         1949 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.372, whole genome shotgun sequence.
ACCESSION   DAELJO010000370 DAELJO010000000
VERSION     DAELJO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.372"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..274
                     /locus_tag="KPB88_003952"
     CDS             <1..274
                     /locus_tag="KPB88_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056157.1"
                     /translation="KQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNALDL
                     FNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            538..792
                     /locus_tag="KPB88_003953"
     CDS             538..792
                     /locus_tag="KPB88_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056158.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            837..1160
                     /locus_tag="KPB88_003954"
     CDS             837..1160
                     /locus_tag="KPB88_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056159.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1719..1913)
                     /locus_tag="KPB88_003955"
     CDS             complement(1719..1913)
                     /locus_tag="KPB88_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0056160.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          808 a          165 c          275 g          701 t
ORIGIN      
        1 aaaacaaata ccaagaaaca atttagaaaa gtttttaaga ataagagtct tatcacatac
       61 aggcgataaa atacatataa agttacctgt aaattttgca aagagaatga ttgagaataa
      121 tgctttggat ttatttaatg gaaaagatga tgtagtagat ggtaaaaaag ttactgaaat
      181 attagttaag gcatttgact atgatttaac tggagaagta gttcacttag agagaaaaaa
      241 tggagacatt attaaagttt caattgtaca atagaattat tttatatgaa ttttattttt
      301 gaatagttat aaataattag aaatttgtaa aaaagtataa tatattatta gtaatggtac
      361 agaatacaca tttataattt atagatgtgt attttttgtt taaaaaattt aaatatataa
      421 aactataaag gttacaattt tggaaataga tgtaaatata tgttgtattg tataataaga
      481 gtaatataaa atctagtaaa aataatcaaa taaatttagc ttatgagagg gataactatg
      541 gaaaataagg attttaaatt aacaatatgt atatttgctt ttgtaattgg agtagtagta
      601 ttattctgtt gtataggttt aggtgaattt agtgcagagc atattattca acaaaatggt
      661 ggaatcatgg atactgacca atataatatt tatctagacc aatctatttc tcaatataga
      721 aattttggtt ctatattagc tctgttagga ggaataggga tattgtttga taaatatatc
      781 agaaaatact agagattatg ttttgtttaa aataaattct tatgaggaga taaaaaatgg
      841 caacagaaat atttactgta aaaattacac ccttagaggc acaaaaaatg ataaaagaaa
      901 atcttgatgc agatttagtt tttagtgatt cttataattt aggagatgat aaatttatac
      961 taattacaac ttttgagaag tattacgcaa gaaataatag tgatgcagga ttaatagtag
     1021 tttatgaaaa taccacagga aaaacagtta ttaaagctac ttcaacaggc agtgcaattg
     1081 gaattttaaa aattgattgg ggtgcaggtg gaaatttact taaaagagta aaaaacatac
     1141 ttgctgatta tattatttaa ttattttatc taactgataa tatgtcaaga aaatctcgta
     1201 aaagaggtta agaacatttt tataatatat agataaataa gaatttgaag gtaaagtttt
     1261 taaggtataa agaaaaataa tataataaaa cgaattggct ttataattct ttataaggca
     1321 aagagcacat ttctttatat aattgttgga tattaaataa tatgtaaata caccatttac
     1381 atataaaagt ttaattaata tgttaagaga aaatgaaatg gaaattacta aaaaatattt
     1441 ttaaattgga taatggttga tattatgttt atacaaaaat catgtagaat tttatatatt
     1501 ttaaatatga tagtttaaaa ttatgaaaga atgataaatt ccagtttgta gggtaatttt
     1561 gataaataat attaataagt atgagtaaaa agtaggagca tattaatata atgtccctac
     1621 ttttttatta ttaaaattgt ttataatatc aacactacat taaaacacaa cctaaatttg
     1681 aaaaacaaat agtttagaaa tatgtacttt tttatctatt atattctttt aaatcgaatt
     1741 ttttcatctg aacataaagt ctcaaatcta tatgcgtctg tcatatcata aaaaccaaaa
     1801 aatataaagc catcacgaga agtaaattca tattgtatat tttcaatttt caacatgttt
     1861 tctacttcgc ttattttttc actatatata atttctacca tataatttat cattgtagaa
     1921 tccctcctat taatatgaaa atatccaaa
//
LOCUS       DAELJO010000371         1947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.373, whole genome shotgun sequence.
ACCESSION   DAELJO010000371 DAELJO010000000
VERSION     DAELJO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.373"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            517..1857
                     /locus_tag="KPB88_003956"
     CDS             517..1857
                     /locus_tag="KPB88_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0056161.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          767 a          148 c          295 g          737 t
ORIGIN      
        1 attgtagatt tattatttac taataaatct acaatagaaa tgcttttgtt gtaagaatca
       61 ttttatctaa tgattgtttt ttataatagt taaatttcca tttgtagtgt ttaaatataa
      121 atctataaca gcatttctat aaatgttata aatcactata atgattcaac tcaaagttac
      181 tgcttaaaga agcagagatt ttatcaaggt atatcatgga aaaaaatatt ttattgtaat
      241 atttataaga ttttattatg gaaataatgt aaaaacttta taaataatat attttatacc
      301 tagttataga taaatttata tagttattat taaaaaaagc aataaaaagt ttaattcaat
      361 ttagcaaatt tagtgatata attaaaaaag aaaagaaata tggacttatt tggaataatt
      421 ttgacaatct atttactaca cgtgtagagt cgtatataat taaattatac ataaaatata
      481 aaaaaataat ttagtaatac gttaggagaa agtattatga taaagtcaag cttattttgg
      541 aatattttca ttgcgatatc ctttatgata gaatggtttc tttgtaagaa aatgctagat
      601 tatacaagtg agaaaaggat ttctaacaaa aaaataaact tttgtgtggt aggtattatt
      661 gtttttgctg acataatata tttttttgat gtacctccaa atataagagt aattatatct
      721 atgataatta ctattgcatt ttataaaatc tcatatgatg tggggtttat aaagtctgta
      781 ttaatatcct taatttattg gatgttacta atcgccacag atgctttgag tatatcttta
      841 acagtttggc ttaattctat aaatgacatg agtagtatgg ggacgggaga tatatacaga
      901 attgagtcta taatattggg aaagatactt ttgattgtgt ctttgctttt atatagagcc
      961 ttaaagataa aacttaatat agaaataaat aaaaggacat tattttatat agttattcct
     1021 atagtcgcta atatagcatc tttttttgta atttttgaat acatatttaa tccagggtct
     1081 ggtagcttaa tacataattt ccaattcgtt atgatttctg ttgtattgct tatatcaagt
     1141 atcactttag ttttaattgc taaaaaatta cagcaatata atactttagt tgtagaaaac
     1201 aatatgatta aaaatacttt gaagtatgaa aatgagtatt atgaagatat agaaaagaaa
     1261 tatttaaaaa ctagattact aagtcatgat atgaaaaatc acatattgtg tataaatgct
     1321 atgattaaaa aaggtattga tgttacttct tatttaaata atttgcaggt agaaattcag
     1381 agtaatgact tgttatttaa tactggtaat tttatattag atgctatatt aagtgaaaag
     1441 aaagaggttt gtgataagaa tgatatagaa ctaaaaatag gaataaattt tagttgttgt
     1501 gaagctgttg aattagtgga tatatgtcat atattttcaa atattttaga caatgcaatt
     1561 gaggcatgta ataaaattga tagtggaaat agagttattt gtgttaaagg tgatgttatg
     1621 caaaattttt atttaataag agttgaaaat actaagataa ataagattat tactaaaaat
     1681 ggagatattt tgacagacaa aaaaggttct cttcatggac ttggaataaa aagtgtaaaa
     1741 aactctgtaa ataagtatgg tggtaatgtt gtaattgaat atgatgataa aaaatttgta
     1801 gttaaaattg ctattccact tagaaatgtg aagaaagcag ttaatcatgt gatttaaatg
     1861 tatataaatt tgatgtgtag aaatttaata tataagaatt aatgtataaa agtgatgcat
     1921 agaagtttaa tatataagaa ttaatgt
//
LOCUS       DAELJO010000372         1929 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.374, whole genome shotgun sequence.
ACCESSION   DAELJO010000372 DAELJO010000000
VERSION     DAELJO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.374"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            61..1815
                     /locus_tag="KPB88_003957"
     CDS             61..1815
                     /locus_tag="KPB88_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG0056162.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT          828 a          164 c          339 g          598 t
ORIGIN      
        1 atttgtaaat aaggggataa tttataagaa aattaaagac cttaatttac aggaggaata
       61 gtgtatatta tgaataaaaa attcaaaaaa ttcagtaaaa aactaagtat attaatttta
      121 gttttgacta tgtttctttt taataattta actgcaaatg cacagattaa agaagattca
      181 ataataggaa ttgatagata tgaaacagca ggacttatag cggatagaca aacttatgat
      241 actgtaatat tagttaatgg agataaaaat ttatctgatg ggctttcatc aagtggatta
      301 gctggagcta ttaacgcacc aatacttttg actaaaaaaa gtgaaatacc aaaagctact
      361 ttaaatagac tagataataa gagtttaaat gttgtaaaaa aagtatatat aattggtggt
      421 tataatacta ttgaaaattc agtagaaaaa gatataaaaa gaaaaggtat tcaagtggaa
      481 agaataaatg gtaacaatag aattgaaact agttataatg tagctaaaaa aataaatgaa
      541 gtaagtagtg taaaagaggt aatgcttaca aatggatttg taggagaggc tgatgctatg
      601 agtatagcac ctgtagttgc aaaaaataaa ggagctataa tacttacaga tgggaaatca
      661 gttccattta agactgaaag tttaaatgtt tacgctatag gtggcaaatc tgcgataaat
      721 gaggatttag taaatgaaac taaagcaact agaatcggag gaaatgatag atttgagacc
      781 aataaaaagg taatggaaaa gttttataat ggagctacag atttttatat aacaaatgga
      841 tatcaattag tagatgcact aacattatct cctttagcca aagaaaaacc aatcgtttta
      901 gttgctgatg gaagtaataa aggtatttta aaaggagcta aaagtataac aaaagttgga
      961 ggtattgatg ccaatatata taaacagtgt ttagatatga ttaaatataa taatatggat
     1021 atgactccaa atattttaaa acacttaact tcagttgaag gaaatgaagt ttctgaaata
     1081 cttatagaat cagataattt aggtgtggtt gttgaaaaga cgaataccaa taaatttgag
     1141 tttgattacg taagtgtaac taatgaaaaa aattgtactt tttctgtata taaagaaaat
     1201 tcttctaata atataaaaaa tggaaagcta gttgtaagtg ctaagaaaaa aatagataag
     1261 aaagatagtg atttgcaaga tatgaatggc gacaatatga taaatgcaaa taaagatagg
     1321 atggtaaatg tgataaaaat tggagttccc gataaaatgt attctaattt taaagtagat
     1381 gttgaaagtg gttcagtaga actttataat attaaaggag gagctactgt tgatgtcaat
     1441 gacggaattg ctaacattgt tgataacaat gtaacaaatc ctttcaatat aaatactaat
     1501 agtgggattt caggtgtaac agctgaaact atatcatctg aaataaaatt tagagcaaac
     1561 aatggaattg taaatgtaac agctacgaat ataaatggca atataagttt atttggaagc
     1621 gataataaag atacttttga tggaatattt aaattaaatc tgaaaaaaga accatcaaat
     1681 ttacacttga agttaattgg aaatgggata aataaattgc cagaaggttg gtctaaagat
     1741 tatattttag gaaatggaca accaattatt gaagttaaaa ataatggcat aaataatata
     1801 tcaattggag aataaaaaca tatgaaaata aagtttatat agttttaaaa attatagaat
     1861 tttaaaatat ttatatatat tctttaaata aaaaattatt ttttaagtga taattaagtg
     1921 tttatcgat
//
LOCUS       DAELJO010000373         1929 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.375, whole genome shotgun sequence.
ACCESSION   DAELJO010000373 DAELJO010000000
VERSION     DAELJO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.375"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(358..747)
                     /locus_tag="KPB88_003958"
     CDS             complement(358..747)
                     /locus_tag="KPB88_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056163.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          634 a          354 c          100 g          841 t
ORIGIN      
        1 tcaatatctt cattaattcc tatttatttt atcatcttaa atacgaaaac actgtgtcat
       61 atttgcaaaa tatatattat catctatatt aaagcttcac aatttatatt taaaaattct
      121 ctttaattac actacctcta tttaaaaagc taccttaatt atacttataa gtaaaggtag
      181 ctttttaaat ttaattaaat agaatatcca ctatcaattt atacttaatt tatttagatt
      241 cacattatag tatcataatc cacttataat catttgtcat attacttaag tagactaata
      301 tattacataa atacacttac atacacttta tattgatgct ataagaaata cgtttgctta
      361 tacgtctttc tcctcttctt ttagattttt tatccactct tcaataagta agatatcatc
      421 tttgctcaaa ttaatatttt catgtaatga caacaataaa ctttttatag aagaatcatg
      481 aatatttcta aaaaaatctc ttgtctcaac tcctatgtat tccttttctt taattaaaac
      541 ttcataatgc gtatatttat ctatcttttg agaatttaga aatcttttta ctactaatct
      601 agataaaaga gtcaaagttg tcgtttgttt ccattgatat ttttgctcca ttcctaaaac
      661 aatatctcta gatgttactt tggaatctga tttccatata aatttcatta tctttaattc
      721 tgattgtggc aactttttta tagtcatgat aaaaccttgc tactcagaat atagacaagc
      781 aatatccata ataaaatttt tcgattaaat acatcttata cggatttata taagctcaga
      841 cttataagat atttttcaat cttaatccta aatttagcta tttgtacacg gtaaatatat
      901 ataaataatg tcaaaattct cttgtattat attgcttaat tttatgaaat attttgtact
      961 cagctatcta ttttggatac ttgtccagta gctctccttt cttttaaact ctttgatttt
     1021 aaatatttgt atttcagtaa atcctacttc ctaaattttt tatttactac aacttattac
     1081 tgtacttcct acttcctaaa ctttttattt actacaactt attactgaac ttctcacttc
     1141 ctaaatttct tacttactat aaattataac taaacttctc acttcctaaa tttcttactt
     1201 actataaatt actactaaac ttcctacttt ctaaatttct tacttgctat aaattattac
     1261 taaacttcct actttctaaa tttcttactt actataaatt attactaaac ttcctacttt
     1321 ctaaatttct tacttactat aaattattac taaacttcct actttctaat tttcttactt
     1381 actataaatt attactaaat ttcctacttt ctaaatttct tacttactat aaattattac
     1441 taaatttcct actttctaaa tttcttactt actacaaatt attactaaac ttcctactcc
     1501 ctaaatttct tacttgctac aaattattac taaacttcct actccctaaa tttcttactt
     1561 gctataaatt attactaaac ttcctacttt ctaaatttct tacttgctat aaattattac
     1621 taaacttcct acttcctaaa tttcttactt gctataaatt attactacta aacttcccac
     1681 ttactaaatt tcttacttac tactacttat aactaaactt cccacttcct aaatttctta
     1741 cttattacca cttataacta aacttcccac ttactaaatt tcttacttac taccacttat
     1801 aactaaactt cccactttct aaatttcttg cttactacca cttataacta aactttttac
     1861 ttcttaaatt tcttacttct taagaataat gttaatttta attttaagat tatagttttt
     1921 gccttgttt
//
LOCUS       DAELJO010000374         1902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.376, whole genome shotgun sequence.
ACCESSION   DAELJO010000374 DAELJO010000000
VERSION     DAELJO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.376"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            7..405
                     /locus_tag="KPB88_003959"
     CDS             7..405
                     /locus_tag="KPB88_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056164.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLIIVLVVLIFIKRKKKKFY"
     misc_binding    841..1104
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1255..1806
                     /locus_tag="KPB88_003960"
     CDS             1255..1806
                     /locus_tag="KPB88_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG0056165.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          804 a          183 c          286 g          629 t
ORIGIN      
        1 gaaaatatga ataagaagaa aaaattgatt gggatagcta tatcttttct acttataatc
       61 agcattataa atccattaca agtagaagca gaagctatta ttgagtctag tgtatacatt
      121 tcttcaataa aagagaatta tacatatgat aatactaaat ttaaaactat taaatctaaa
      181 agtagacata aaagtaggaa atataataaa agtaaagtaa agttgaaaaa acgaagtata
      241 agaactaaaa agtatggata tcaaactata aataaatata aaagacaaaa cgcaagaaga
      301 cataatatcc taacaaaatt aatattgtcc ataatagtca tatttttgat tatagtttta
      361 gtagtgctaa tatttattaa aagaaagaag aaaaaatttt actaaaaata attaaaagaa
      421 tatcattttt ttaatcattg gtatctattt aataatatga tgtaattaaa atggagtaat
      481 attctgtttc aaaggaggca tataaataca ggaatgagta ttaaatgatg gttaattaag
      541 taaatttatt gataaaggta cttaattaac tttgaagaat ttaattcctc atgaatcaaa
      601 atataaaatt acattaacag ttattcacaa aattaatata agaagaatta gagccaaaga
      661 gcagactatt agaccattaa agtaattatt catagaattt tgtatatgaa aagaaatgta
      721 tagtataatt tacttttttt ataaaaaata gattacaaaa tatatttttt ataaaaaggt
      781 attgacattt tttagaaaaa agaatatcat tattttaata acaaaattca atacaataaa
      841 ttaaatccta tgacaaggaa gagtagttaa atttaggttc agtatagaga gctgaggatg
      901 gtggaatctt agcaagaaaa aatttgatga atggccctta gaggaataaa ttgaaatcat
      961 agttttgaga gtagacttta tcgtcagtcg cacgttatag cgaataaggt atgtaagtac
     1021 ctgataatga gatgtatgaa aattgaattt tatattaatt tttatataat ttaggtggca
     1081 acgcggataa tctccgtcct attagtttag gacggagttt tttattttac tgaaaattta
     1141 aaagatagaa ggtggtgaga aagatgttat gaatttcaaa agctaaagat gatgatatag
     1201 taggttggca aaaaaataaa ttgaaaatta aaaaaatagg aggaagtaca aaaaatgaaa
     1261 acaaatacaa aaaaattaac tttaaatgca atacttttag cgatgggatt attaatacat
     1321 caaatcacac cagctatagg attaccaatg aagccagatg taccactagc aatgttattt
     1381 gtaatattgg ttttaaatag agatgattat aaaacttgtt tgatagcggg tatagtaaca
     1441 ggaatattta cagcacttac atcaagtttt ccgggaggtc aaattcctaa tgtaatagat
     1501 aaaactctta cagcaaatat agtattttta ttaatgaata ttagttataa tatgcctttt
     1561 ataaaaaaat tagctaagaa aacacaagat actgtagtag ttgcaataat aatgccaata
     1621 ggaactcttg taagtggaac tatcttttta ttagcagctc aagctatagt tggtcttcca
     1681 ggggcttcat ttacagcttt attcttagca gtagtattac cagctgtttt aataaatcta
     1741 gttgcaggaa tatttttatt caagattgtt agtctatcaa taagaagagt tagttatcaa
     1801 gcttaaacac gaaataatat tttactttat aaagcattat aataactaaa taacataaga
     1861 ataaagtaga ttataactat tttattctta tgttgtttaa ag
//
LOCUS       DAELJO010000375         1832 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.377, whole genome shotgun sequence.
ACCESSION   DAELJO010000375 DAELJO010000000
VERSION     DAELJO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.377"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            182..247
                     /locus_tag="KPB88_003961"
                     /pseudo
     CDS             182..247
                     /locus_tag="KPB88_003961"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(763..1515)
                     /locus_tag="KPB88_003962"
     CDS             complement(763..1515)
                     /locus_tag="KPB88_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0056166.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1574..1816)
                     /locus_tag="KPB88_003963"
     CDS             complement(1574..1816)
                     /locus_tag="KPB88_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056167.1"
                     /translation="MSPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDRSMSISDI
                     CKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT          703 a          296 c          156 g          677 t
ORIGIN      
        1 ccacaattgt taaagcaaaa acctaatgac tgatatactt atatatatta catatacttt
       61 taatttcaaa ttaaaagtag ctaaccccaa tttaaaaagt agttaagtcc tatttaaata
      121 gaaaagaatg aaataagaaa tatactgtat aaaaatgcat aaaaacctct actaattaga
      181 tacaactata atagaatata tttattatta taataacaaa agaataaaaa aaattaattt
      241 agcataagct tgttaaatat aaatttgaat atgaatttaa agttatgtaa aatcaaaaat
      301 acaggaatga aaattccatt cctgtataaa atctaacttt tgagtctcag tacaatctta
      361 ttttgagaca atcttttttg ttttatattt tcttatattt aaaatataac gcatatctta
      421 tgcaacatat tcatattttt ctttatattc tttcatcgca tcattccaag cgtcttgtac
      481 tgttacacct cttttattag caattttttg tgccagttca attatcttca tagctcttct
      541 tgatatgttc ataaacattc ctcctacaaa ttaccatata tttcatatat ataaatcaag
      601 gttatatttt tatgatatat aatccttcta ttgtaagatt acaatttata tatgtattat
      661 atatgaacta tattagaaga taattagaat ttttataaaa ctatacgatt aggttaaatc
      721 taaaaagttt acaattactt ataaatttaa aaggtctata aactaaacta atgtttgata
      781 taccaatttt acgaccataa acaatgccat tactaaaaat attggtttta tcatctttga
      841 acctttcttt atagcaactt tagcacctac ataagctccc attatcatta ctaatgcaaa
      901 cactatagca attttataat ctacttgacc atttatcatg aataataaca atgatgttat
      961 attacttgtt aagtttaata tttttgtatt tgcagaagca tgtaaaaaat catatccata
     1021 tatttttata aacccaaaag tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata
     1081 aaaacccatt ataagagcca taagcatccc tagtcttaaa tttttcttat taacagcttc
     1141 aaaattatct tcttgaccta aatttttaga aataaaggtg taaatagcca ctattaaaat
     1201 cataaccaca accaaaacat ttaatatctc ttcacttatt gataatacac atctcacacc
     1261 tagaacacta ccaataatag taaatggtac taattttttt aaaaggttgt tattaatttt
     1321 tccagacttt gcatatctat aggcactact tatacatcct gctgatgctg caaatttatt
     1381 tgtaccaatg gcaagatgta ctggcactcc aattgccata agaacaggca tgcttataag
     1441 tcctcctcct cctgctatag aatctacaaa agcagctaaa aaccccccta tacataaaaa
     1501 aataactgaa aacataaaat tcctccatca ttctctttca atttttctta caatatatat
     1561 tatataatct tatttaatac ttattatcca gcatattctt tctatattca tttggagtta
     1621 tactattaaa cttcttaaat atttgtgaaa aataactact gttattaaaa cctactttct
     1681 tacaaatatc actaatactc atgcttctat ctaacagata ttttttagat tcttctatcc
     1741 taagaagatt tatatactca gttattgaaa ctttatttct ttcattaaaa atagaactca
     1801 aataatttgg acttataaaa aaaacatctg at
//
LOCUS       DAELJO010000376         1823 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.378, whole genome shotgun sequence.
ACCESSION   DAELJO010000376 DAELJO010000000
VERSION     DAELJO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.378"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            122..1171
                     /locus_tag="KPB88_003964"
     CDS             122..1171
                     /locus_tag="KPB88_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0056168.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1321..1668)
                     /locus_tag="KPB88_003965"
     CDS             complement(1321..1668)
                     /locus_tag="KPB88_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0056169.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          676 a          245 c          283 g          619 t
ORIGIN      
        1 aaactgaaaa ttattattaa gtttttgtag aaatattata agtatagata catttttgta
       61 acaactacaa taaatattta atactatata ctattcgtat gtcttatggg aggtatttat
      121 tatgtttaaa aaaatagtcg caatagcaag ctctatcatt gtaagtacaa tatgtctaac
      181 tggatgtgat tttaactcta atgacaaaaa agatgaatct tcttcgtcta aaaatctgga
      241 taaagtaact atagcagaag ttactcactc tgttttttat gctccacagt atgctgctat
      301 tacgaaagga ttctttgaag atgaaggtat aaagattgac cttataaata cccaaggtgc
      361 agataaaact atggcagctt tgatttcagg tgaagcaaac gtaggtttaa tgggtcctga
      421 agcatctata tatgtatata atcaaaacaa aaacgattat gcagtgaact ttgctcaatt
      481 gacaaaaaga gatggtagtt ttctagttgc taggaaaaaa atgcctaatt tctcttacaa
      541 tgatttaaaa ggaacagaaa tattaggtgg tagaaaaggt ggagttccac taatgacttt
      601 tgaatatgtt ctaaaagaaa aaggtcttac tattggagaa aatctaaaag caggcaatgt
      661 taatgtaaga actgatgtac aatttggtgt aatggctgga gcttttgcag gtggagaggg
      721 tgactttaca acagcatttg aacctacagc aacagcaatg gaaaaagaag gtagtggata
      781 tatagttaca tctataggaa aagattctgg agaaatacct tttacagcat actctactac
      841 gagatcatac atgaaaaaca ataaagattt aattcaaaga tttacaaacg ctgtttataa
      901 aggtcaatta tggattcaac aagcatctag caaagaagta gctaaggcta tgcaaccttt
      961 ctttgaagat atatctttag aagatttaac aacagttgta gatagatata aatcaataga
     1021 tgcatggtct caaaatcctg tattaaaaga ggaaggtttg aagaaattga tgactgttat
     1081 gaaagaagct aaagaactag ataaggaagc accttataaa gatatcgtaa atactgagtt
     1141 tgcaaacaat gctataaaaa atattaaata aaatatatta gttttagcta gatattttct
     1201 tgtttttata atgaaaatta acaaaaatat aaaaagagcc ttttaaaaga ctctttttat
     1261 atttgagatt aaagtttata taataaaata gatattaaag tactaactgt acttatatat
     1321 ctatcctctg tattctgctc cagcattaat ttttgtaatt tgaacggcta ccatttcttc
     1381 attgcttcca ttagtaattt catgccactc tcctgttggt acaaatacag tatcgccgtt
     1441 ttttacatca cattcatgct caccaagttt cattttacct tctcctttta ggaacacaaa
     1501 tatttgttca ccattagggt gtctatgagc attaagaact tgattaggtt taaaataata
     1561 aacttctgaa gttatatcgt taccagcaaa tactggtatt tttggaactg ctgtatcatt
     1621 atattgagct aagcttccgt ctaatagatt ctttttattt tcatgcatac tagaaatacc
     1681 tcttttctat attatagatt tactattatt ataccaatat aagaaaatag tattctcact
     1741 ttacacaata ggaataaaat tcttttattt ttaaatataa gtataatact atttttatag
     1801 ttagaaaaat acaatcctta taa
//
LOCUS       DAELJO010000377         1807 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.379, whole genome shotgun sequence.
ACCESSION   DAELJO010000377 DAELJO010000000
VERSION     DAELJO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.379"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      <1..64
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(586..744)
                     /locus_tag="KPB88_003966"
     CDS             complement(586..744)
                     /locus_tag="KPB88_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056170.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(1103..>1807)
                     /locus_tag="KPB88_003967"
     CDS             complement(1103..>1807)
                     /locus_tag="KPB88_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG0056171.1"
                     /translation="RYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVL
                     VDIGSNKDILKNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNH
                     IIWQDKGSYLECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYM
                     GQYAKIKMDFNSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLAS
                     RILFYNPDGEDFFEYK"
BASE COUNT          637 a          275 c          192 g          703 t
ORIGIN      
        1 cgcaaagcta tagggactaa aacttatata aatatcttat gagttatgtc agccagttgc
       61 caaagagata ttgttctttt tgtttttatg aaagtttttt aaggggataa taatttatat
      121 gtttagagat aaaatggata aatgtacaca tatattgact gcttatatta gcagttcgta
      181 tgattattgt aattttttgg atacaattta cattccatat agttaatata aaacaattac
      241 ttcaaaaata taatccgaat tggcagaact catttacatt ccatatagtt aatataaaac
      301 cccaaaataa attgagtatt tccaatgcct acacatatac aaatctctca aatttgcagt
      361 gaaccatgag tagtgcaatt gataacactt atcacaagtc ctcaatgcct tatatttcaa
      421 ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa aatttctatg actttattat
      481 atcataaaaa tattattttt gaatatctgt accaatttgt gatataataa aagcaagaag
      541 aactacaatc tattttgctg tagagtggag ttcataattt agaaattact tagttttatg
      601 gaatctgatt tttaaatcaa attcccaacc actcttagtt gccgctttga gtggtttttt
      661 acgctttcta aatagtttgc tagctatata aactattaag ctagcagata gacttgctag
      721 tatgccttgt aaaaaattat ccatgtatat tcacctccct tcttaacgtc gggaggataa
      781 ttttttgata catgaactcc actctataaa ttgtagatta catcttcttg ctaaaaatat
      841 tataacatat aattcttaca tattttacct atttattact ttttacatta taataatatt
      901 cttttaatca aaaaaacacc tactattaaa gtaaatgttt ttaagtattt ttaattttaa
      961 aattcatata gttcatatga aactaaatat aataactcaa catatgagca atatcgagac
     1021 atttacatac cacatagtta atataaaacg aaaataaaaa aattaaactt ataaaaccct
     1081 tattaattta catacccact agttatttat attcaaaaaa atcttctcca tctgggttat
     1141 aaaataatat tctacttgcc aatccttgtt catatttata attttcttta tcacctacat
     1201 aagaaaatgt taatgtcatg cttcttgatg gatctaatga aattatatct aaataaatag
     1261 tacatttttc tcctggcttt agtacaccaa aactattaaa atccattttt attttagcgt
     1321 attgccccat atataatgtt gaattagctt ctccataaac ttttaatttt gttacatcta
     1381 aattttgtgt acttgtattt tctatattta taggaactgt tgggtcttta tatttttcag
     1441 tataatattc aaggttatca tcataacatt caagataact tcctttatct tgccaaatta
     1501 tgtgatttga atcaaaatca atctttaatc cacatattat atttttattt gaagatggaa
     1561 gactaatatt attgatggta ttttcacact ctagcataat tttatcactt agatttccta
     1621 tcccagtcac ttttgcagca tttttaagta tatctttatt acttccaata tcaactagta
     1681 caataggatt atttttagcc attggtgaag caactaaagc ataaactaaa tcagtaccac
     1741 tagctatata aaattctttt gcgccattat agaacttatt tactattttt ttattagtat
     1801 catatct
//
LOCUS       DAELJO010000378         1799 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.380, whole genome shotgun sequence.
ACCESSION   DAELJO010000378 DAELJO010000000
VERSION     DAELJO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.380"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            440..1645
                     /locus_tag="KPB88_003968"
     CDS             440..1645
                     /locus_tag="KPB88_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0056172.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          664 a          228 c          229 g          678 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttaatttttt tatttatcat gtattcttta tctttaattt ttaaaactta atgctaagta
      181 aacctgttta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatcaaaata taatataaat actatgctac
      421 attttaggag gattttatta tgagcaaaaa agtattaata atgtctgctt ccactggtgg
      481 tgggcataat agggctgctc tagctattaa agaagaactt acttccaaaa cacttgatgg
      541 agagccaatt gaatgtgaaa taatagatag tttgaaatta gttaataata caatggataa
      601 aataatttca agaggatacg aaaaatctgc tatatatact cccaaagcct atggaagtgt
      661 atatagatta tctgaaacaa atcttttgtc aaaaaatgaa tttaaagata atctactaat
      721 aacattcatg gctaaaaaat ttaaaaagct tattagaagc gaaaaaccag acttgattat
      781 aggaacgcat ccttttccaa tgattgctct aagtacattg aaaaaaaact ttaatttaca
      841 taataatgaa tcaaatgctt acacagagca tttttataaa cattatacaa ataccattaa
      901 tgttcctccg cttatttctg tattaaccga ttatactact cattctacat ggatacaaaa
      961 tgaaattgat tactatattg ttggtcatga atatgtaaaa gaattattgg tgtttgatgg
     1021 agttgaaccg agtaaaatta gaacatttgg aatccctgtt gaaaaatctt ttctctctca
     1081 cagagataaa gatattgttc tttctgagtt gaatttatct cctgataaat taactgtttt
     1141 acttatgggg ggtagctttg gggctggaaa tataaaagag acacttgatg aattactgga
     1201 tactgataga gactttcaaa tacttgtaat tactggtaag aatgaaagcc taaaagaaaa
     1261 aatcgaaaaa aaacttatgt ctcgttatca tgataaaaat gtatgtgtcc ttggatacac
     1321 tgataagatg aatgatattt tagcttctat agatgtatta gtaagcaaac ctggtggact
     1381 tactacaact gaggctcttt taaaagatgt acctatgata gttccttatt atataccagg
     1441 tcaggaagaa gagaatcttg atttcttatc taattgtggt gcagcactta gaactacaaa
     1501 aaaatataac ttgtcaatat tactaaaagt tttaatagat gaccctagca gattagaaat
     1561 gctaaaaaga aatattaaat caataagaaa gtcaaattca gcccaaaata tagctaattt
     1621 aattttaaat ataatttctg gttgataaaa taaataaaat ccctatagta tatatattat
     1681 ctattaggga ttttttattt tctaattatt taatattaaa ttggttcata aaagtcttct
     1741 tcataatcat actgataatt tttataatta tttctaaatt cagcattttt atttcgtac
//
LOCUS       DAELJO010000379         1766 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.381, whole genome shotgun sequence.
ACCESSION   DAELJO010000379 DAELJO010000000
VERSION     DAELJO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.381"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     repeat_region   65..1476
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=396..424
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT          643 a          283 c          179 g          661 t
ORIGIN      
        1 attatatttt aaaggagaaa atgctgtata aattattggg gtattttacc tatacttgtt
       61 gcaaatttac ataccacgta gttaatataa aacaaatttc aaagatattg gacaacgaat
      121 aaaaattgca atttatatac catatagtta atataaagca tatttgtgtc gatatatagt
      181 tctaactaag tataagattt acatacctca tagttaatat aaaacaatct cttatactga
      241 atacattagt ttttaaagtg ttatttacat accacatagt taatataaaa ctttagattt
      301 acctgctaat actactgacg ttattttcta atttacatac cacatagtta atataaaaca
      361 ctttatggta ctacagaaac ttctcatatt ccagaattta cataccactt agttaatata
      421 aaacgtttcg ccaatggctt tggacttgaa tgtatttttc gatttacata ccacttagtt
      481 aatataaaac ttatgctctg caaatgttca acgtcttaat acctcaattt acataccact
      541 tagttaatat aaaactagcg ttcataatgc tttcagtgtt gtcagcaatt tgatttacat
      601 accacttagt taatataaaa ccccataatt tgccattact gatgtgttta agtagctatt
      661 tacataccac ttagttaata taaaacctga gaaaattcta tattaaattc aaatccagca
      721 agatttacat accacttagt taatataaaa ccattagcaa aaaaggctgg tttatttcct
      781 gcttatatat ttacatacca cttagttaat ataaaacggt tgataatgca attgatgctg
      841 gtggaagggt actagattta cataccactt agttaatata aaacgccact ttacccatgc
      901 atattgttct ccaccttctt tatttacata ccacttagtt aatataaaac gtgcgttata
      961 aaagtctaag ttttgttctt caaatgtatt tacataccac ttagttaata taaaacaaag
     1021 ataagttttg taataattgt aaaaaagtta tacatttaca taccacttag ttaatataaa
     1081 actagatgtt ccaattgctg gtgatgttac tgaccttgta tttacatacc acttagttaa
     1141 tataaaactt tctactgatt ttgataactg ctttattgtt cgtcaattta cataccactt
     1201 agttaatata aaacttggtt aattcttatc aagttgattt ttctattaga tttacatacc
     1261 acttagttaa tataaaactt tactttagaa gaagtgtata ttattataat cccagattta
     1321 cataccactt agttaatata aaactttgaa attggttttt ctaattttaa agtaacttgt
     1381 gatttacata ccacttagtt aatataaaac tctctcctga gtttgataat tgttttatcg
     1441 ttcatcaatt tacataccac ttagttaata taaaacccca aaataaacta agtattttca
     1501 gtacttatac acatacaact ctctcaaatt tgcagtgaac catgagtagt gcaattgata
     1561 acatttatca tacaccctca atcccttaca ttgcaactgt taaaccctac tttatcaaaa
     1621 atatcgctca ctgcaaaatt tatatatttt tattatatca taaaactcaa actataaaat
     1681 taaaatggaa taatttatca atttaagaac acctatatat ttgtaaatac ttactatatt
     1741 atctattcta atagttcttc ttctag
//
LOCUS       DAELJO010000380         1764 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.382, whole genome shotgun sequence.
ACCESSION   DAELJO010000380 DAELJO010000000
VERSION     DAELJO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.382"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(41..793)
                     /locus_tag="KPB88_003969"
     CDS             complement(41..793)
                     /locus_tag="KPB88_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBG0056173.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLKSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQVMKPKADYFDALVERNLLTN
                     IRDTAKELGVKEKTFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYGHSDTQ
                     TLINPKGREAFRLLLIKDGLVKEKEYQITLLG"
     gene            complement(793..1026)
                     /locus_tag="KPB88_003970"
     CDS             complement(793..1026)
                     /locus_tag="KPB88_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBG0056174.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNK"
     gene            1205..1600
                     /locus_tag="KPB88_003971"
     CDS             1205..1600
                     /locus_tag="KPB88_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056175.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
BASE COUNT          568 a          247 c          214 g          735 t
ORIGIN      
        1 actacctcca taatctttaa ttttcaaagt actttcaatt ttagcctaat aaagttattt
       61 gatattcttt ttcttttacc agtccatcct ttataagtaa taatctaaat gcttctcttc
      121 cttttggatt tattaatgtt tgagtatctg aatgtccgta tggtgttgta aattctttca
      181 gttcaaaata ctgcatcttg ttagagtaag gttttatttt gccttttaaa tctctgtaac
      241 aatatttctt ctctattaac cataaaacaa atgttttttc tttgactcca agttcttttg
      301 ctgtatctct tatattagtt agcaagtttc tttctactag agcatcaaag taatctgctt
      361 ttggtttcat tacttgattt tctaattgta attgctcttt ttcttcaact tcgattaata
      421 actgttgcag tgcttcttta tatgtagttg gtaatttagg ttgttgttct tttaactctc
      481 gctccatttc ttcaaactta gttacataaa tcgctgtaaa tataattccc ttttcacctg
      541 tcattttatt agctaccatg tcacaacctt ttttagttaa taagtagcaa ggttgaattt
      601 tattttgagt atttatataa gtactttcta tgaagaaatc ttgactcttc aaatttgatg
      661 agtcctctaa aatcttcttg tatcctctta tatctcttaa taaattatcg tgcttctttt
      721 ctattaattc tgctacttct ctactttcaa ctaaaaattg attattttgc ttgattatgg
      781 ttagattctt cattatttat tgcccctttc ttcttttatt gctattttag caacttcatc
      841 tgaaaaaaaa taatccgcag atacattata taatttagat atctttttta tttcacttgc
      901 tttaaattcg ttttttcctt ttaattttaa tctaaaacca tatgagctaa gacctaatat
      961 atctgccaca tttttttggg tgtgtctatt ttccttcatc aatccttcta atctatttaa
     1021 gtacattaaa tcacttcctt tttgctattt tggcaacttc atatttatat aatatcaagt
     1081 tttgtgatta tagtcaatac ttttgttgct tttttagcaa aaaaaattgt aataattaat
     1141 tttgttgcta taatataaat aaaagttgct attttggaat taatataaaa aggggttgtg
     1201 caaattgaat aggataaaag aattgagaga agaaaaaggc atctcgctag acaaattaag
     1261 cgaggattta catataaaca aatctacact atcaaggata gaaaatggtt taagagaacc
     1321 taagaaaagt acaatagaag aatatgcaaa ctattttgat gtgtctacag attatttatt
     1381 aggaagaact gatgttagaa atagcttatt tataaataaa aacgaaaaag attatgatgc
     1441 tgaaaatttt aaaacagaaa aggagcttat tgagaatatg tatcttgacg aagatatgaa
     1501 agaagttttt aatatattta gcgagctaag cccagacgca agagaaaaag cattaaaagt
     1561 tgcagaatta tttttactag acgaaaaaaa taaaaaatag tttatttctg aaaatgaata
     1621 taataaaaaa gaggaatcat tcctcttttt tattgatttt atgtatttct ttcactttta
     1681 ttttatattc attgatttta tttttatcca attctttcaa ctttttcatt aataagttta
     1741 actttaaaat atcataatat ttcg
//
LOCUS       DAELJO010000381         1731 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.383, whole genome shotgun sequence.
ACCESSION   DAELJO010000381 DAELJO010000000
VERSION     DAELJO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.383"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            181..891
                     /gene="rgaR"
                     /locus_tag="KPB88_003972"
     CDS             181..891
                     /gene="rgaR"
                     /locus_tag="KPB88_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG0056176.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          686 a          141 c          254 g          650 t
ORIGIN      
        1 atatctagaa aagtatagga atttatcgta atttaagttt aaatattaaa gagaatttct
       61 ataagtagtt taatcgacaa tttaaattta taaagaaatt taaatttgaa agctagtgag
      121 tacagtttgg aaattgaaat aaagtaaact aaatgatatt ttagatttgg aggtgatatt
      181 atgttcaggg tagttatatg tgatgacgaa aaaatacaaa gaagcatact aaaagagttt
      241 acagaaaaac ttttgtctga aagatgtcta aattatcaaa tactggaatt ttcctgtgga
      301 gaagatttaa tatccaaata cccagaaaaa attgatatta tatttttaga tatccaaatg
      361 aaagatatca atggaattga ggtggctaaa aaaataagaa agtttgatga aaaagtagaa
      421 attatattta caacggcttt ttcagagtat gccccccaag gttatgaggt tcgtgcttat
      481 agatatttag taaagcctat agagtatagt aactttgcaa tgggtgtaaa tctttgtatt
      541 gataaccttc aaagaaagaa ggagcgttat atagttttaa atagtaaaaa aggatttaat
      601 agaatactga tagattctat tttatatgtt gaaactgtaa agagagattt agtcgtacat
      661 acaataggta gagattataa agctaatatg agcatgaaac aagcagaaaa attactgtgt
      721 gaaaatggct tttttagatg tcatacaagt tttattgtta atttaagaag aatagaagat
      781 cttagggata atatgattac tattaatgaa caatttatac ctataagtaa gtatagactt
      841 aaagatttaa aagtagcatt aacaagcttg ttatgtgata taatgtatta attttaaaaa
      901 ggtaattgaa aatatcgtaa aaaagattta atgtcatcac agggctattg attgaaactg
      961 tcttaatata taaaatgtta tttatattaa gacagtttta ttatgcaaaa tagcagttta
     1021 tagattttta atatggttta acatgtatat gtagaggttc cattatagta gcatgattat
     1081 taattagtag cttgattatc aatatactat tattgatttt tatatgcatt atatgtattt
     1141 ttagaatatg attacctttt taaaataatg actatttata aaactctata gatatttgtt
     1201 tcatttagct acgcatactt taattaatta tattaatatt taatggagta caatataaat
     1261 attaaagcga catcttatag tttattgaac ctatagtaat gaattaataa atagtttaat
     1321 tctatgaaaa gatttccttt tatgcaattt atttattgaa atatgatata atatataaag
     1381 gtgtaagtat tagttgttaa aataatattt atattagttt ttatacatac ttggtataag
     1441 tttttcatat attgattaaa attgatgttg atttattatt tttagtattt tttcaccttt
     1501 tgattcatac tttgtagaat ggtgtatttt ctaaaactga ttataagaca agttcattca
     1561 aattagcatt tttatatcat ataaataatt gttcccccta aataagattt agatgtatag
     1621 tattaaaatc aaaaagtata aaataaaatt gatgcttaat catagataaa tattaataaa
     1681 atatacaagg ggattgtcta ataaaaagat aatctccttt tttacaaata a
//
LOCUS       DAELJO010000382         1724 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.384, whole genome shotgun sequence.
ACCESSION   DAELJO010000382 DAELJO010000000
VERSION     DAELJO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.384"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            342..1634
                     /gene="brnQ"
                     /locus_tag="KPB88_003973"
     CDS             342..1634
                     /gene="brnQ"
                     /locus_tag="KPB88_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0056177.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          626 a          214 c          303 g          581 t
ORIGIN      
        1 ggagagtgat atatacagta tataaagcaa tacaacttaa taataaatcg aataatctaa
       61 aaagtcttaa tattttaatt attcggaata gttaatttta tcaagaaaaa ttagagaaaa
      121 aacaaaaaac caactatgtt taaagtatac aggttgattt tgtttaatta agaaagttgt
      181 attaaatatt gtgaagattt tataaattat gaaacataat tgacaaattt aagtcaaaaa
      241 tgtataatta aataaaatct cgtcaaaagg ttttcattta attaagagat tggacttatt
      301 ttttagtaaa aggtagtaaa attcaaatgg gggtgcttta aatgggtgag aaacaaatca
      361 attttaaaga tgtaattgta attggatttg cactatttgc aatgtttttt ggtgcaggga
      421 acttaatctt ccctccatat ctaggagttt tatctggaag tagttggcta gtagctttca
      481 taggattcct ttttgcagat ggtggtttgg cattattagc agttatagca gcaactaagt
      541 tcaatggaga cacatcgaag atgttctcaa gagcaggcaa aggattaagt attgcattag
      601 gatgtgcaat ggtaatctgt atagggccac ttttagcaat acctagaaca gcagcaacaa
      661 cttatgaaat gggtatatta cctacaatag gttctggaat aagcccagta atattctcta
      721 tagtattctt tgctctagta ttggttttaa caataagacc atcaaaagtt gtagatatag
      781 ttgggtcaat attaactcca gctttactta tagctttagc agtattaata gttaaaggta
      841 tcgtatctcc attaggagaa ataagagaca gttctttaat acaaaatgta tttgcagaag
      901 gtataactca aggttatcaa acaatggatg cattggcagc tagtgtattt gcatcaatta
      961 taatcatgtc agtaatagct aaaggttata caggtgagaa agaaaagatg aaagccacaa
     1021 taagtgcagg tgtaattgca gtaataggaa tggcattagt ttatggtgga ttatgttatc
     1081 ttggagcaac tgtgtctcaa caatatggac aagatgttca acaaacagct ttaatagttg
     1141 caataacagc agctttactt ggaactacag gtaaaatttt attggctatt atagttgcat
     1201 tagcatgttt aacaacagct ataggtcttt cttcagcagc tggtcaatat ttctcaacac
     1261 ttacagatgg aaaattaaag tatgagcata tagttatagt agtttgtgta tttagtgcaa
     1321 tcatatcaaa ctttggagta agtacaataa tacaattctc ttctcctata ctaagtatgg
     1381 tatatccagc aactataact ctagttatac tagcattatt tagtaataaa attaaaaatg
     1441 acaacgtgtt caaatgtgct gcatacatgg cattattagt aagtgtatta acagttgcaa
     1501 caagttttgg tgtaaaaatt cctctagtta atagcttacc atttgcttca ttaggattta
     1561 actggatagt tccagttgta atagcaggaa taataggaaa ctttattcca tctaaaagca
     1621 gtaaaactgc ataataataa aaactaattg ataattatgg aattgccttt aaattatata
     1681 ggcaattcct ttttatattt aaaagaatta ttaaattatt gtag
//
LOCUS       DAELJO010000383         1693 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.385, whole genome shotgun sequence.
ACCESSION   DAELJO010000383 DAELJO010000000
VERSION     DAELJO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.385"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..836)
                     /locus_tag="KPB88_003974"
     CDS             complement(<1..836)
                     /locus_tag="KPB88_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0056178.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPT"
     gene            977..>1693
                     /locus_tag="KPB88_003975"
     CDS             977..>1693
                     /locus_tag="KPB88_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0056179.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAAAIGNMVGPSVGG
                     FILSIFDWNVIFFINFPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLS"
BASE COUNT          587 a          247 c          208 g          651 t
ORIGIN      
        1 gttggagata tattataatt ctttttaaat aagcgactaa aataatttat gctatcaaag
       61 ccacatctca tagcaatttc tgtcatattt aaatctgttt cctttaaata atatatagct
      121 ttatcaagcc tgattccatt tataaaatcg attgtagtct tacctgttat ttgcttaaat
      181 gttctacaaa agtgatgtgc actcaagttc acacgactag caagttcttt taatgtaatt
      241 ttatccatat atttttcttc tatcaaatca aagatacatt caaatttttc taaagaatac
      301 tttcttttta taaattcatc ttcagaatat attttatcaa tatgacttct taataagagt
      361 actattaatt gatagatatt agcttttata gataactcat atccaacttc tttattgtga
      421 tattcatcaa gtatagttgt aatacatttt aaaatcagag aatcattttg aattaaatta
      481 ttaaatacaa tatgatttaa agccaatggt gctaagtact tggtttgtaa caaatcaact
      541 tgattactaa aaaggaatgt gggttcaata cgaatactat agaattttaa attatctgat
      601 aagctttcta aataatgtaa ttcattactg tttgctatta caatatttcc aggcactacc
      661 ttaaaattat ccaatccaca ttcaagatat gctataccat caataaaata atatatttgt
      721 agatgctcat gccaatgtct tttaaagttt gctccttttt taatgtcatc ctttaaaaaa
      781 atatctataa agttttcttg acctttcctg ttaaaatatg tatttttatc cttcattatt
      841 cccccctcat caaaatagtg ctaattataa gcaatatagt tattgtattc attatttcat
      901 atcgctatta tataaaatgt aactatcatt gtaaacatta acataatgca gtcattataa
      961 taaggaggta ataaaaatga caaactcttt taaaaaagat gataatagaa aaaaaatcac
     1021 agttttattt gtagtcattg ccatgacttt tatggcaaca ttagattcta gtattataaa
     1081 tgttgctctc ccagttcttg caagcaaact aaatgtatct ctagcatcta ttgaatgggt
     1141 aatagcaagt tactcaatta ttatttgttc aacactatta ttttttggaa gacttggaga
     1201 tattattggt aaatcaagag tatttcaagt aggtactatt ctctttacat cagcttcatt
     1261 actatgtgga ttaagtaatt ctttaacttt acttatagtt tgccgcttta ttcaaggaat
     1321 aggcgcttct gcctacatgg caaacaatca tggaattatc acagaactat ttccaaaaga
     1381 aagcagagga aaagccctag ggattttagt tactgcagct gctattggaa atatggttgg
     1441 accatctgtg ggaggattta ttttgtcaat atttgattgg aatgttatat tttttataaa
     1501 ttttccaatc ggtcttattg taatcttttt aaatacaaaa tttcttccta atagtaaaaa
     1561 atcttcagaa aatatggata aaactggtgc tatacttcaa tttttaggaa caacattatt
     1621 ttttagtgct ctaattagtg ctcaacaaac aggattacta aatccatata tattaattgc
     1681 actattatta tca
//
LOCUS       DAELJO010000384         1688 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.386, whole genome shotgun sequence.
ACCESSION   DAELJO010000384 DAELJO010000000
VERSION     DAELJO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.386"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(80..1372)
                     /gene="eno"
                     /locus_tag="KPB88_003976"
     CDS             complement(80..1372)
                     /gene="eno"
                     /locus_tag="KPB88_003976"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG0056180.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          495 a          343 c          180 g          670 t
ORIGIN      
        1 ctcttttata tcttataaga tgagcttaaa tatcttatat tctctcttca ttaatctttt
       61 ttagtaacca ttttaataac tattttttta agttatagaa agattttaat ccacaatatc
      121 tagcttgctc tccaaccatt tcttctattc ttaataattg gttgtactta gcaactctat
      181 cagttcttga tggagcacca gtttttattt gaccagcatt tactgcaaca gctaaatctg
      241 ctatagtaga atcttcagtt tctcctgatc tgtgagatat aacagcagta tatcctgctc
      301 tcttagccat ttctatagca tctaaagttt cagttattgt acctatttga ttaactttaa
      361 ctaatataga gttagctact ccattttcaa ttcctttttc taatctctct gtgttagtaa
      421 caaataagtc gtcaccaact aattgtaatt tatttcctaa tttttcagtt aataatttcc
      481 atccatccca atcttcttcg tctaaaccat cttctatagt tattattggg aagttgtttg
      541 accaatcttc atataaagca accatttcag cagcagttaa ctcttttcct tctcctgcta
      601 aaacgtattt tttagtttct ttgttgtaca tttctgtagc agcaacgtca agacctaaca
      661 taacgtcttc acctggctta tatccagctt ttgttatagc ttcaactatt aattctaaag
      721 cttctctgtt tgaacctaag tttggagcaa atccaccttc gtcacctaca ccacaagcta
      781 atcctttttc tcctaaaact ttctttaatg aatggaatac ttctgctccc attcttaaac
      841 cttctttgaa acaacaagct cctactggta atatcataaa ttcttgaaca tcaacgttgt
      901 tatcagcatg ctctccaccg tttaatatgt tcatcattgg aacaggtaat tgtttagcat
      961 ttactccacc taagtattgg aataatggta aacctatttc atcagcagct gctctagcta
     1021 ctgccattga aacacctaat atagcattag ctcctaattt acctttgttt ggagttccat
     1081 ctaattctat catgattgca tctatagctg gttggtcaaa tgcatccatt ccttctattt
     1141 caggtgctat tatttcattt acgttagcaa cagctgtttc tacacctttt cctaagtatc
     1201 ttcccttatc tccatctctt aattcaacag cttcaaaagc tcctgtagat gctcctgatg
     1261 gaactattgc tcttcccatt gctccatctt ctaatactac ttcaacttct acagttggat
     1321 ttcctcttga gtctaatact tctctagcat atactaattc aataactgac atttgtatat
     1381 ctcccttcga ttttctatta gttaaatttt catttactgt aaacatccgt ctactatttt
     1441 aatctacttt ctatattata gtacataaag ttgcttttga aaattaggta caaatttatt
     1501 tgatgggtaa atttgtaaaa gttcctattt ttattttatt ttttataata ttttaattta
     1561 tataatataa aaaaacttgt ctaaaatcca aaaccaatct taaatacaca aatattataa
     1621 ctagatttaa aacattaaac ataaaatgta caactaatga ttctaaaatt tttgcttata
     1681 taatatat
//
LOCUS       DAELJO010000385         1682 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.387, whole genome shotgun sequence.
ACCESSION   DAELJO010000385 DAELJO010000000
VERSION     DAELJO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.387"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..212
                     /locus_tag="KPB88_003977"
     CDS             <1..212
                     /locus_tag="KPB88_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0056181.1"
                     /translation="ATVVGWGYNDGKILICDIKDYVPGQTQNLYVVGGVACEKIGSMT
                     KEKFTMIKGNDRFDTLYKALEFINK"
     gene            462..953
                     /locus_tag="KPB88_003978"
     CDS             462..953
                     /locus_tag="KPB88_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056182.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
     gene            956..1540
                     /locus_tag="KPB88_003979"
     CDS             956..1540
                     /locus_tag="KPB88_003979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056183.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
BASE COUNT          758 a          148 c          284 g          492 t
ORIGIN      
        1 ctgcaactgt agttggttgg ggttataatg atgggaaaat actgatatgt gatataaaag
       61 attacgtgcc aggtcagacg caaaatcttt atgttgtagg cggtgtagca tgtgagaaga
      121 ttggttctat gactaaagaa aaatttacta tgataaaggg taatgataga tttgatacac
      181 tttataaagc actggagttt ataaataaat aaaatcaata gagttaatat attttacatt
      241 ttgactacaa ttttatgaca gtatcacaac aatttataaa ataaaaaatg atatattaaa
      301 tataaccata agtcatctac aaactttaaa aaatcaggta tatgaagagc agttgagtat
      361 atagcaataa gtggtaggag gaggaataaa aaataatagt gcaatgaaag taattataat
      421 gtagaatagt attaaataat attattttag ggggagttaa gatggcttat gaaaatggga
      481 ctaatttggt agcgtatttt cctaactgta taaaaaaata cgaaaaggat aacaaaatat
      541 ttgaaaagac aatactatta cttaatttat atatgcagat gccaacttgg ccacattgta
      601 ttcatgaatt taaagctaaa gcaaatgaaa ttttgggaga agacccagaa tttataggtg
      661 agtatggaat taaactgaaa gacttttata atgaaggtga aaatgtaaat ataagccaaa
      721 actttaaaaa tgatttgtat actttttcaa atagtacata tgatatatta gataactcat
      781 atattcagag aatcaatcca attggcttat attctatatc ttctttggct agagaaaatg
      841 attttatttt gaaactattt agaaatgata aaaaatcttt ggaattagaa ctatctaaat
      901 cagaaataca atcaataatt gaacatttag agtctatgat agaggaagaa tagttatgaa
      961 gggtgaatat aataaagaaa aagaaaaagt aataaagttt aattccaaca aggataaaag
     1021 taaaatcaac aaatatacag actattataa tactttaaat agtactttga tggagaaaaa
     1081 taaatgtatt ggagataatt ggtttttagc tactacttca gaggaggtag gtaatatgaa
     1141 tgaagaagaa aaaagaatac tagaaactga aaaaagaata ttagagactg aaaaaagagt
     1201 acatcaaaat aataaggaag taaaagaaag tatagataag caatttaatg acataaaggg
     1261 tatttttaaa gaatacaaga atgatttaga caaagataga atagaactaa aaaatgattt
     1321 agctagagaa agaattgagt taaaaagcga tttaaaagaa gctataaacg aacataaaaa
     1381 ggtgacagaa aaggatgtaa gtgaaataaa gaatagtata aaaggtatag aagatagaat
     1441 tgattcaact aataaatgga ttattgcatt atgtataact actataatag gaattgctac
     1501 aatggctatt acaataggta tatctatatg gccaaagtaa cacaaaaaga ggtaactaga
     1561 attaaaatag ttatctcttt ttatacttaa acaaaacatt gaaaaaatag taaaaaatat
     1621 taacaggata cataaaacta tagtataacc tgtaataata aaacgaacga aaattatcat
     1681 ac
//
LOCUS       DAELJO010000386         1670 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.388, whole genome shotgun sequence.
ACCESSION   DAELJO010000386 DAELJO010000000
VERSION     DAELJO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.388"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            324..1595
                     /gene="serS"
                     /locus_tag="KPB88_003980"
     CDS             324..1595
                     /gene="serS"
                     /locus_tag="KPB88_003980"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0056184.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRAYMGVSVIK"
BASE COUNT          707 a          169 c          299 g          495 t
ORIGIN      
        1 tttgaaaagt aatataaaaa aatttatata aaagttaata actatacaag attgataaag
       61 aaaaagtcaa aatagataat tgattagcaa aatagatata attataatga agttaaatct
      121 agcaatattc tcctaaaatt aaatttcatt tatttaatta aatataattt gacaatgggc
      181 aaaaaagtga gtaaaatata aataaaataa aaataacagg ttcggataaa gaaaaaggtt
      241 tcttaacctc aagcataaag tatattatct aagttatttg agattaagag ataaatccaa
      301 tcttaggtac tgaggaggaa aaaatgcttg atataaaaag aataagagaa aatttagatt
      361 gtataaaaaa agcaatggaa agaaggggcg aaaaagactt taaccttgat gaagtagtta
      421 agttagatga tgaaagaaga aagattcttc aagaagttga agttatgaaa aatgaattaa
      481 atacagcatc taaaaatata ccaaatctta taaaagaagg taaagatgta gaaaatgaaa
      541 agattaaact aaaagagtta tctgataaaa ttaaggtaat agaccaaaat cttaaagaag
      601 tagaagataa gatggaatac ctattaatga gaataccaaa tgtaccacat ccagaagtac
      661 cacaaggtga aactgatgaa gataatgttg aagtaagaac ttggggaaag actactacct
      721 ttgattttga atctaaggct cattgggaaa ttggaacaga attaggtata cttgattttg
      781 aaacagctag taaaataact ggttcaagat ttactcttta caaaggatta ggtgctagat
      841 tagagagagc attattaaat ttttacttag acacgaatac aaaagtaaat ggatatacag
      901 aagttattcc accatttatg gcaaatagaa atagtttttt aggtacagga cagttaccta
      961 agtttgaaga agatatgttt aaaatagaag gtctggacta ttttatgatt ccaacatcag
     1021 aagttccttt aactaatata catgctaatg aaatacttaa atttgaacaa ttaccaataa
     1081 attatacagc ttatactcca tgttttaggt cagaagctgg ttctgctgga agagatacaa
     1141 gaggtttagt aagacagcat cagtttaaca aagtagaaat ggttaaaata gttgcaccag
     1201 aagaatctta caatgagtta gaaaagctta ctaacaatgc ggaaactatg ctacaattat
     1261 tgaatctgcc ttatagagta gtaaagatat gtacaggtga tttaggattt acggcttctt
     1321 ttaaatatga tgtagaagtt tggatgccaa gttacaatag gtatgttgag atttcatctt
     1381 gttcaaactg cgaagatttc caagctagaa gagcaggaat aagatttaaa agagataaag
     1441 attctaaagc tgaatatgta cacacattaa atggctctgg gttagctatt ggaagaagtg
     1501 ttgcagctat attagaaaat tatcaacaag aagatggttc tgtggttgtt cctgaagttt
     1561 taagagcata tatgggagtt tctgttatta aataagagtt aaattatgta tttttaagca
     1621 atacaataat atagaggtaa taaaaaataa ttaatataaa agtgatactt
//
LOCUS       DAELJO010000387         1625 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.389, whole genome shotgun sequence.
ACCESSION   DAELJO010000387 DAELJO010000000
VERSION     DAELJO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.389"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            37..1017
                     /locus_tag="KPB88_003981"
     CDS             37..1017
                     /locus_tag="KPB88_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0056185.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            1220..>1625
                     /locus_tag="KPB88_003982"
     CDS             1220..>1625
                     /locus_tag="KPB88_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056186.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHM"
BASE COUNT          652 a          130 c          277 g          566 t
ORIGIN      
        1 aaaatataat ataggaaaat tgtatggtaa ataaatatga aaaatagttt taaaaattgt
       61 attagtttaa gaaataaatt tataattata tcaattgtgt ttctattaat cctattaatt
      121 gtatcttata tatttggcaa attagtgtat aatagttcag ttggtttaga gcagttaata
      181 aaaaacgaag atatagtaaa aatattttct gagagaaaag ataaaccgct ggagaaatta
      241 aaaaactaca agacatctga attaatgata caaagttcta ataattacaa gcttgaaagt
      301 ctttttataa cttcaaatat aaagacaaga gatactatga tacttgttca tggaattgga
      361 agtagttact atgaaatgtt aaaggtagca tatagatatt tagataaagg ttataatgta
      421 ttagtttata atcaaagaaa tactggtaat agtggtggag ataattacac atttggtctt
      481 tatgaaaggt atgatttaga tagtctagtt aaatttgtaa aaaataaatt tcctgaaggg
      541 agattaggtg tgcatggatt ttccatgggg gcagggacag ctgctatgca ttcagagata
      601 aattcaaaag atgataaggt cgatttttat atactggatt ctccttattc tgaaatgaaa
      661 gatgcaatta gaatgggagt actagaaaaa cgaataccag atatattaat caattatgtg
      721 gtgacttgtg gagatttata taataagttt aaatctggat tttggtacag tgatgtaaaa
      781 ccatatgagt cagttgagaa atctaatgta cccatactgt ttatacatgg aacaaaagat
      841 actgtttgta attatcaaaa tagcaagaaa atgtatgatt tagttaaaca tgataaaaaa
      901 gacctatggc ttatagaagg tattggtcat gttgatggat ttgaacatga tagtacagta
      961 tacttcaata aaatatttaa atttatagat agtaatgttt taagtgataa aagttaattg
     1021 aatattagta gcttatttat ttttgtattt attaaaaaat agataggcta tttttatgtt
     1081 taaatataag ttttgtttag taatgtgaat taatttctag taaaaatgaa aataactacc
     1141 aattaaattc atttatacct caaataaaag agttagtatt agattacaat caaatctaaa
     1201 gaaaagagag gtagtatata tgaaaaacaa aaataataaa gataaaaaca tgttgaatat
     1261 taaagtattg agagttctag gagtgctatt aatacttttt gattatacgg gtgatacttt
     1321 gaatttttat aatttcttcg caaaaccatt agtatcgggt aatttatata agacaacagg
     1381 tactgactgc attgtatttt tattatttat tgtagagtgt gtatacctag tgggaagata
     1441 tatattaaaa gttaagataa attggagtga gattattgaa aatgagaaag gtaattttat
     1501 aaataacttg ttggttgaaa aaatacaaga agaaagcaac attgtatata atttattaaa
     1561 ctcatatgtg ttgccacata tatataagaa agcaagggta ttgatgttta aaacatatca
     1621 tatgt
//
LOCUS       DAELJO010000388         1590 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.390, whole genome shotgun sequence.
ACCESSION   DAELJO010000388 DAELJO010000000
VERSION     DAELJO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.390"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            193..>1590
                     /locus_tag="KPB88_003983"
     CDS             193..>1590
                     /locus_tag="KPB88_003983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056187.1"
                     /translation="MNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKNINNT
                     HITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLGFSDL
                     KNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAETDYP
                     GNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRKRIES
                     VNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLINKNI
                     CTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNLDFLE
                     ETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDNNTKF
                     NTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKDSKTI
                     KNLIMFGFYDLKYLDI"
BASE COUNT          659 a          206 c          163 g          562 t
ORIGIN      
        1 atttaaatgt taactacaaa tatccattat attcttgata atgatatatg tgaaatttat
       61 tttaataaac aaaggctaac aaattctaag tattatcaag ataatattaa tgtattaaat
      121 tatattataa atgaaaaaaa taataactta attatcaatt ataatgacaa atacatatat
      181 gctatttatt tgttgaatat agctataaac tcatacttaa ataatacatt aaacccaaat
      241 aatcacattc ttacaaaact cgataaaggt atgcccctgc tctacaaggg tcaaatagtt
      301 atatttaaaa atataaataa tacacacata actttaggac ataaaaataa aacggataca
      361 acacttatac ctattgactc ggcttatatg cttataacct ataatggtca tttagaagtc
      421 aattctatgg gcaaaactcc agcatctaag acttttcaaa caaagaatgt tctatcatct
      481 ttaggttttt cagacttaaa aaatatgact ggagtaataa atgattctac actgattata
      541 ttgccttgta aagatgatat atctgactta gttagtaata ttaaaataaa agatattaat
      601 aatacttata agtttactga actatttcca tgttcatata tttcatcaac tggtgccgaa
      661 actgattatc ctggaaatca cgctaagcaa atacctttac ttaaatttac aactaatatt
      721 tctagtgcat ttgaaattat taaatctgat aagtctataa aaaatgtatt tataataggt
      781 gattatccaa tttataagca tataaatgat tttgaaagaa ttttaaatag aaaaaggata
      841 gaaagtgtaa atattgtaac ctcaaattca aatataagca atatattaga tttaccaaat
      901 atagataatc taaatatata ctcttggtct aaagatgttc tgttaaccta tagccaagat
      961 tttgaaaata cctctacttt tcagaaatta tggctagata aacttataaa taaaaatata
     1021 tgtactactt ctgttgattc taatatctct gacttgatac atcaggctag aaaatcactt
     1081 tattacatat ctaagtatga ttttgatatt tccataaaaa actcacttat gatgtgtagt
     1141 tactctcttc ttaaaatctt agaaaataca ccttttaacc tagatttttt agaagaaact
     1201 ataaagcttt taaatttaaa tatagcaact ccttcagcga agctagaaaa tataaaatat
     1261 ttaatttcaa gtactagccc agatttagaa ttaagtgcat tatttgatga tgttatagtc
     1321 aaatttgaaa gaattataaa tgaattatct gataataata ccaaattcaa tacactaaat
     1381 aaattagtat cttcatataa atacaaaaca tttacaccaa atacacctgt tatattgcta
     1441 caaaaacaat atgaagctat tatacttaaa aatttattaa aatctagaaa tataaattta
     1501 caagttattt ctgctgaaaa atttaaagat tcaaaaacaa taaaaaatct tattatgttt
     1561 ggattctatg atttgaaata tttagatatt
//
LOCUS       DAELJO010000389         1560 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.391, whole genome shotgun sequence.
ACCESSION   DAELJO010000389 DAELJO010000000
VERSION     DAELJO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.391"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            87..>1560
                     /locus_tag="KPB88_003984"
     CDS             87..>1560
                     /locus_tag="KPB88_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBG0056188.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEHHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQN"
BASE COUNT          638 a          178 c          220 g          524 t
ORIGIN      
        1 tatctgcctt aattttagtc ttaaatttag agtgtaaatt gaatgcattt agattataat
       61 atatacagtg aggtaaggtg aataatatga aaatagatat gatatataaa ctaataagaa
      121 gaaatacaga accatcaaga tggtcaagag gtgtaaaata ttatactaat caattagttg
      181 attatataga catagatatt gatgacctac aaaataacct gttgacattt cgtggtggag
      241 tcaattctga agataattat agcttttatt ttaattcaat aactatagat ataaagcact
      301 ttaagataat tgaaaatgat tgtagttgcg ctgactgtca atcaaagggc aaaagtattt
      361 ttatatgtaa gcatatagtt gctattgtga taaaaagctt aaaacaaata aaaaataaaa
      421 aagatgttcc tcctattcta atagatatat taggtgcaga taaggataag ctttctaata
      481 caaattctaa aaccagctta gatttaagca agcaattact ttctttgtta gacaatacta
      541 aaagagaaag ggttaattta gatgtattga taaatgtgga ttcaaataaa gcaattgctg
      601 aatttaaaat aggaattgat aaaatgtaca ttttaaaaaa cattactgaa tttgcatatt
      661 caaaacttaa tggatatgaa ttagattttg ggaagaaatt tacttacaac cccaacattc
      721 attattttgc tgatgaagat gagaagttag tagatattat agaagaatat gggacaaatc
      781 tttcatacaa cttttcttca tcacattcaa aaaaatttat ggagataaat gcaagctctt
      841 taaaacaatt tttaaatgct attaagtaca aggattttac ccttattttt tctaaagagg
      901 aacatcatcc aaagatatta aatgatactc tacctgtaag gtttgatttt aaaaatatag
      961 aagataaaat agtcttatat tctaaagatt cactacctgt tccacttact aaaaaaaatg
     1021 atgtactttt atatgatggc aatatctatt tattatcaca taaaaaagct catgattatt
     1081 caaaaatata tcaaatatta tcaaagacta aagagttaaa atttgataaa gaggattcta
     1141 aggatgtttt aggattgcta gttcctagat taaaatctat atctcaagaa gttcatttag
     1201 atgataacat aaaaaataat ataacaaaag attttaaatc tgagttctac tttgatatga
     1261 ttgatggcaa catatgttgt gatgtaaagt atatatatga tgatgaagat aaaaaatttg
     1321 tgctccccaa catacaaaag gaagctacta ttgaaaataa acttacttct tcacaattta
     1381 caaaggaaaa taatcattat gtgtttaaag gaactgacca tgacctattt acatttttag
     1441 atttacaatt agaatctcta aaggaatttg gagatatata ttattctgag aagtttaaat
     1501 tgaaaaaaat atataacgct tcatcaattc aagcttcaat caatcagact aaccagaatt
//
LOCUS       DAELJO010000390         1554 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.392, whole genome shotgun sequence.
ACCESSION   DAELJO010000390 DAELJO010000000
VERSION     DAELJO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.392"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            10..492
                     /locus_tag="KPB88_003985"
     CDS             10..492
                     /locus_tag="KPB88_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0056189.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            547..1326
                     /locus_tag="KPB88_003986"
     CDS             547..1326
                     /locus_tag="KPB88_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0056190.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
BASE COUNT          686 a          140 c          250 g          478 t
ORIGIN      
        1 gtgttaaaaa tggatactaa aaaatataaa gataaattat tgaaagaaag agaaaattta
       61 actaatttag tagaggatat gaaagataat actttatttg gagatacaac taaacataca
      121 agtgaaaagt attcatcagg agaattatct agttatgata atcatatagg agacatggga
      181 acagatttat atatgcaaaa tatgcaaaac agtctaataa atcatgaaga gggaagattg
      241 tatcaaatag acttagcatt atctaaaata gagaatggaa catatgggat ttgtgattta
      301 tgtcataatc aaatagattt agaaagattg gatatattgc cagatacaag tttatgtaat
      361 gattgtgcaa agaaagaaaa taatttatta ggtgatgcaa tgcaaaaccc tgatgtagaa
      421 gattctaact tttattcaga agacctaaca aatttaactg atttaaataa aaatggaata
      481 ttgagagatt aaaaaactga cattataaaa tatattgcta gaatgaatta gaaaagaggt
      541 aatgacatga agaaaataat atggggtata tgtatatgtt tattatctat agtaggtgta
      601 tatacatata atcataatac acataatcaa gatagttcaa aagaagcata tagtttaaat
      661 gacaatataa aagacgatta tgaagatgta ataataaaaa aaggaaatga ggatgaaaaa
      721 attttagcat tgacttttga tgatggtcca gatgaggatt ttacaccaca agtacttgat
      781 atattaaaga aaaatgatgt aaaagcaact ttttttgtag ttggagaaaa agtagaatat
      841 aacaaagaat tattaaaaag acaatatgat gaggggcatg aaatagggaa tcatacattt
      901 acacatataa atgttgcaaa aaatagttat agtagtgttg aaaaagaaat aacagatacg
      961 caaaatatta taaaaagtgt aataggagta gaaccaaaga tatttagacc tccatataga
     1021 gccatgagta aaagtgtctg tgatattata gtttctaaag gcatgaatat aattctttgg
     1081 tcaaacttag accctagaga ttggtctaac cctggagtag atgcaattat aaatactatc
     1141 cttacaaagg tccaaaatgg taatataata cttttacatg attataacac taaaagaaat
     1201 gataaatcac aaactataca agctttagaa gtagtaattc ctaaattaaa agagagagga
     1261 tataaattcg taacagtatc tgaacttata caacatttag ataaaaataa gcaagtgcaa
     1321 aagtaaaaat atttgtttga agattagtgg tgattgtaag tttaaattac agacatcatt
     1381 aatctttaat ctataaaata cacaaaaaat attgattagt atggtatttt taaatttttt
     1441 atacataatg atatgatttt aaatatgtag aaatctacaa tataaaaaaa gtagtactat
     1501 tttaaatttt gttctagctt tattaagaaa atacaatttt aataaagttg ctct
//
LOCUS       DAELJO010000391         1542 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.393, whole genome shotgun sequence.
ACCESSION   DAELJO010000391 DAELJO010000000
VERSION     DAELJO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.393"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            150..1388
                     /locus_tag="KPB88_003987"
     CDS             150..1388
                     /locus_tag="KPB88_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0056191.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVHERKKYENVRYWNPICEI"
BASE COUNT          657 a          137 c          261 g          487 t
ORIGIN      
        1 taagaatata aatagatatt ttaagggatt agttgttaaa ttaggaaata ttaaaaaaaa
       61 tatatattta tatttaaact attttgttat ttatataaaa atacttatct agttactagc
      121 aattattagt agaaatgggg gaaatagaga tgaataaggg attttacaaa ttaaattcag
      181 tggtattgaa ctttgatatt aagaatgatg ttaacaagat aagatttaat cattatataa
      241 tgagaaaaga aaacatacaa agacttcaag gaactctgcc aaacggacaa ttttatatga
      301 cagtgagaag agctgctaca gatttacaac tttctatttc tactataagt aggcttgtaa
      361 aagagttttt ggatttaggg ataataagat tagtgtctaa gggtgtaaaa ggtaatcgtt
      421 ctgtatatag ttacgtaagt atagagttta atggaagttt atttgatgat agtaaatata
      481 tggaacttga ctgtgtatgt aatgatgaat ttattgataa tagtggcata gagtttgatt
      541 gtgatagcaa agataattct tttaatggag atataaatgt agaaactgtg cattgcatta
      601 atgagtattt ctctgatgat gatgtagagc ataattataa caatttccat aacaaagata
      661 ctataaagga taaagccatt tctaatatta gcactatcaa aaaatatatt gatgaatctt
      721 ttgatacaaa taaatcagaa aattctaact tacaacagga gtcaataaat ttcaacttta
      781 atgatacatg caatgaaata gataaaagaa cgaataatag tatagaaaat aatcaaaata
      841 aaaggcctat gagattgagt gacatcagtg ttttaagtga taaaggtatg acaaataaaa
      901 agaattactt taatcataat catggtatac aaaaagggac taaatctgta acaaagaaaa
      961 aagaatcctt aaataaattt aataaaaaaa atatacagca aagcattata aatatgctaa
     1021 ataaaaaatc aggtaagaat tttaaaatcg attctccaat aagcataaaa cttatcaatg
     1081 aaaggctaaa ggaaggctat gtcttagaag atttctacaa agttattgag gtaaaagtgg
     1141 ctaagtggaa aaataccata atggaaatgt atataaggcc agaaaccttg tttagctata
     1201 agtttgaatc ctatgtaaat gaaaacatat ctatgaaaag tcaaagtaat gttcacccat
     1261 gtaaagaagg caatagtaat gatagcagta attatataca ttctgattat acaacatatg
     1321 atggcagtgt gcatgagaga aaaaaatatg agaatgtaag atactggaat cctatatgtg
     1381 aaatttaatt agtttaagaa tgtatttatg ataaataaga tattaataga gaatagtgag
     1441 ctatagcaaa tgatatatca atagttggta gtaagttata ttaagcaaga tattactatc
     1501 aggtagtgaa ttatattgaa cgatatatca ataattggta gt
//
LOCUS       DAELJO010000392         1537 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.394, whole genome shotgun sequence.
ACCESSION   DAELJO010000392 DAELJO010000000
VERSION     DAELJO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.394"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(510..893)
                     /locus_tag="KPB88_003988"
     CDS             complement(510..893)
                     /locus_tag="KPB88_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056192.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            complement(987..1376)
                     /locus_tag="KPB88_003989"
     CDS             complement(987..1376)
                     /locus_tag="KPB88_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056193.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
BASE COUNT          500 a          255 c          124 g          658 t
ORIGIN      
        1 ttttctaatt aagtgaatat aaaaagctat gaacaatatt actcaaatta ccatcaagag
       61 taacataaat acatatctcc atcaatgatt aagaaattac ttttttattg tatcaatttg
      121 tctttcagag catgacacaa aaggaactaa aagataacaa aatcatcagt gttaaatact
      181 tcgagtttca gagcaaagga gttctaaaac aaaataaaaa caccccgctg aatttatcat
      241 tcagcaggat gttttttact tattaatcca taagatactg gcaaatattg atagtatcaa
      301 accagtataa catagaagta agatagacct caaatcctgt atttatgtgg atttttggca
      361 ttctatattt tattcccatt ctattgattt tctttttaat ctgataaaac cttatctatt
      421 acattaattt tcttctatta tttctatatt ttctatattt tttaacacgc tataaaacat
      481 attagtatca ttttagtatc acattcattt tattcatctt cttcccaatt tttcacccat
      541 tcctctaaca tatctaattt atcatcactt aaattttctt catcatttaa agcagtgaaa
      601 aaacttttta aagaattatt atgaaaaaaa ctaaagaatt tttttgtttc aacttttaaa
      661 taatcttttt ctcttacagc gatagtataa tatgtacact tgcttgtttt ttgagaatac
      721 aaatagtttt tctttgcaag cttagacaat attgttaatg tcgtattttt attccagtca
      781 taaatttgtt tcattgcttc cacaacttct cctgatgcta ccttatttcc atctaatccc
      841 caaataaact tcattacttt taactcagaa tctggcaatt tcttgaatcc cataagctac
      901 accccctttt tgtaaattta tgtatgttta tgtatattta aatattataa caaatttatt
      961 ttaaaaataa ttaatatata tataaattat tcttttccaa ttttttccat attttctata
     1021 tattttttta tttgttcttc tgtcatcttt tcttttttat gcatagtggt aaacattcgt
     1081 ataaaaaaac tatcatattt agttaccttt ctatcttctt ttattgcatt taaatattct
     1141 tttttagtta ctagcacttc ataactaaac tgaaaaccta tcctcttctt ttttaatgtt
     1201 ttcattttta ctaatctttt taatagaatt tctgtagtac ttttcctcca cttatatttt
     1261 tctttcattg cttctattat ttctttttta gataaaatag aatttttcct ccacaagtat
     1321 tccatcaaca tcaattctcc cctgcgtagt ttattaacta cttcttgttg tttcatttta
     1381 tgtcactccc tcaaattgtt ctaatctaaa tcaataataa aaccttctga tttcaattta
     1441 tacaggtaag ttttaccaaa tattctacgc atttagtctg ttttcttaga agaaaataac
     1501 tttcatatct ataaaattat taaattttca aacaatt
//
LOCUS       DAELJO010000393         1484 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.396, whole genome shotgun sequence.
ACCESSION   DAELJO010000393 DAELJO010000000
VERSION     DAELJO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.396"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            49..783
                     /locus_tag="KPB88_003990"
     CDS             49..783
                     /locus_tag="KPB88_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0056194.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(926..1417)
                     /locus_tag="KPB88_003991"
     CDS             complement(926..1417)
                     /locus_tag="KPB88_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0056195.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          541 a          181 c          226 g          536 t
ORIGIN      
        1 tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat
       61 aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      121 ttagcaggtg ctggagaatg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa
      181 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      241 gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      301 acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa
      361 gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat
      421 atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt ttctgttgaa
      481 catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggaaataat
      541 ctacactggc cagtagatag atattttgaa gaaggagtac gtaagtcaaa ttttctagga
      601 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca
      661 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      721 cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga
      781 taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa ttacactaat
      841 ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa agcattcgtt
      901 gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg tttccatttt
      961 cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta ttatcatttg
     1021 gatgtatacc tattttatcc atttttaata aatccttatc aaatacaact tttacaacag
     1081 cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt
     1141 tttgaagtct ttcttctgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat
     1201 cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag
     1261 catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca
     1321 tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg cttaactcat
     1381 ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat tagaaattta
     1441 ttttatatat aaataatatt atcacagaaa actttaaaag aaaa
//
LOCUS       DAELJO010000394         1470 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.397, whole genome shotgun sequence.
ACCESSION   DAELJO010000394 DAELJO010000000
VERSION     DAELJO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.397"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(297..608)
                     /locus_tag="KPB88_003992"
     CDS             complement(297..608)
                     /locus_tag="KPB88_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056196.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(664..1257)
                     /locus_tag="KPB88_003993"
     CDS             complement(664..1257)
                     /locus_tag="KPB88_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0056197.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          535 a          203 c          189 g          543 t
ORIGIN      
        1 aataaactaa ttcaggaagt atagaaagct aatctaaaga agtatagtaa actaatttag
       61 gaagtataga aaattaatct aaagaagtat aataaactaa tttaagaaat atagaaaatt
      121 aatttaaaga aatacaatac attaattttt gagtaacatt aaattaatgt aagtaaaata
      181 atgttcatat gaagatttaa aggtgcattt tctgaagcta agtatatgtt gtagagtgtt
      241 atgtctctct atcctgtgaa gtttaatagc tcaagaaaat gcactattta tgatgattat
      301 tttgttttat ttccaatttt ggaaataact attctaatta aatctaaaat tataccataa
      361 ataagaaaag aaagtataga tgctatgtac caataaatcg aaattgaccc tgtaattgtt
      421 ctacatggga tttgcatacc tgaatccata aaaacaaaaa aactatcaac tctggaaata
      481 atagacaaaa taggatttaa acctattaga acaatacttt ttgagtcttg accaatttga
      541 tgcatataaa tctcaaataa tgcaattaaa aaaaaccaaa atgtaaaaga tttaataatt
      601 tttttcatca aaatacctca cctaatataa attctgtttg cactatttgc ttactacaac
      661 tatttatctc cattcagtca catcacttaa ttgtaatcta ggtcttcttg atggttcttc
      721 atcaggataa cccaaagata ttgctgccac taattgacta tctgtattta accattcaca
      781 taattcacga tatgcaaagt atacatcaca aatccaaaga ctacctagac ctaattccac
      841 agctgtaagc gacatattct gaattgcagc tccaattgat tgcatgtttg ccatttcata
      901 gaatcgttct tcaggggata gcttttctaa aggtgattta cctaatatat ttaaaataaa
      961 aattgttact ggagcttgtt tcatgatttc aactgtgtaa tttgcaccag cgatatgttg
     1021 acgacttcct ggaagaagcc cattatcatt aatttcattt tgaatacctt tgctcatggc
     1081 tttaagcata gattcttttt ccttttcagt gatgacaaca aaacgccatg gttgcctgtt
     1141 tttagatgaa ggtgcattta ttccagcttc aattattttc tctatagttt catgtgaaat
     1201 gctttgattt ttatattttc tgatacttcg tctttttgat atagaatcac ttatcatttt
     1261 aagtaacctc cgtttatttt ttggaaaaat ctatttaatt tttattttct attttaatta
     1321 ttttagcatt aatcagaaaa aaagtcattg attttagtat acaattatta atgattatat
     1381 taagaattgt atcaaaaagt atgtgattat actaagggat tatattaaac attatattta
     1441 aaattgtatg aaaggttgta tttaatatta
//
LOCUS       DAELJO010000395         1462 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.398, whole genome shotgun sequence.
ACCESSION   DAELJO010000395 DAELJO010000000
VERSION     DAELJO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.398"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            319..810
                     /locus_tag="KPB88_003994"
     CDS             319..810
                     /locus_tag="KPB88_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056198.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            838..>1462
                     /locus_tag="KPB88_003995"
     CDS             838..>1462
                     /locus_tag="KPB88_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056199.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKCGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIE"
BASE COUNT          640 a          120 c          201 g          501 t
ORIGIN      
        1 taaattattt aagttaaaac ttggaattat ttataaaagg attatttatt ttatctaaat
       61 tttgcactta aagaacatct tttcccttaa gactgaccgt tagtctataa aaattataat
      121 tacttataaa aaagtgaata gagagatata tatataattt tcagaaagac ttttacttgt
      181 aaattgaaat taatgaagta aaaaaggatt ctttcttaaa agtaaataac aactagatag
      241 tgaataataa aaaatggtaa tttgaaataa tttttatgaa tagtattaat atatagatac
      301 agggagattg atgcgattat gtatgaaaat aataaggtaa tatacaacct tgaaagatgt
      361 gaaaaactag aaaagactct ttatttatct caatttaata catataaaaa ttttaaatta
      421 gcttctaaaa aaactgtaat aaattcatta gagacatatg tttacaatat gaatattaac
      481 aatataagtc ttgttgatac tatagaatgt atatcattag aagatgaaca acttacagga
      541 aagaagttga ttttagttgg tgatatagat attgatgcaa tattagatta tgcaggaaat
      601 aaaagaaata gaaattctag taaaaaaaat acatttaaat taaaaatacc ctttagtaca
      661 tttatacaaa tgcctagaaa gatagaaaat aaagataaaa ttaatttaaa gtatcttata
      721 caagatatta cttcatctat tttagatgat aatttattta ttagtgttac tgcaattata
      781 agttatgaaa atagttccaa gatagagtaa tctttaaatt atagaaaggt ggatgcaatg
      841 agaataacga aaactacttt taatgatgta ttttcaaatg atgcaaatgg aagtattata
      901 aaagtagaaa aatatgttga taaaaaatgt gcatctgtat ttgagatttt gacctacact
      961 attattgtta caaatacaag taggtacaaa actggaaata tatttttcaa agattatatt
     1021 tctaaatata tagagtttat aaataatact gtaaaagtta atggcattat aaaatgtgga
     1081 ttagacccac agcaaggttt ttacataggg agaatagatg caagttgtaa aaaaataata
     1141 tcttttaaga gtgtagtttt accaaattct gcacatagga ttataaagaa tagtgctaat
     1201 atatactatt attataagtg taatctagat aaattcccaa caagaatatc aatagaaagt
     1261 aatagagttt atactaatgt aaataaaatt gtattcaaac aattgaatat aagtaatact
     1321 ttaaaagctc ctaaaaatct aagagatata ttaaaagtaa atgtaaatag taaaatattt
     1381 gatgttaaac ctatgaaaaa tcttataaac aaaggtagta atttgaaatt atgtaacttg
     1441 atagtttttg ggtgtataga ag
//
LOCUS       DAELJO010000396         1436 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.399, whole genome shotgun sequence.
ACCESSION   DAELJO010000396 DAELJO010000000
VERSION     DAELJO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.399"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..529)
                     /locus_tag="KPB88_003996"
     CDS             complement(<1..529)
                     /locus_tag="KPB88_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG0056200.1"
                     /translation="MMNNELNTIILETLSNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEK"
     gene            690..1343
                     /locus_tag="KPB88_003997"
     CDS             690..1343
                     /locus_tag="KPB88_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG0056201.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
BASE COUNT          478 a          171 c          199 g          588 t
ORIGIN      
        1 atttttctga aatatttttt aattggtcag ccattgaagt cagacttaat aaggctatca
       61 ataaatcatc tttattttct ggatttatac taatattttc aaaattagac tctataaagt
      121 ccggtatatt ctttctttga ttttctttta ttaacatgaa tttttcgtaa catgaaacta
      181 aatctttttc actgaaatta tccttttcat acacaccatg taagattctt ttaatttctt
      241 gtattgtaag tgtatttttc atagaataaa taattatcat ctgtaaaatt tgttctttag
      301 tatacttttt tcccttaact ggtttgatta atccctcttt gctgtaatta tttatcatgg
      361 ttttagtaag aattttatca ctttcaaatc ttttattagc agaaagctta ttgtcaatta
      421 gagaaataat ttgatccata tataagtcaa tagatggaat atcgtttgaa gttatatctg
      481 cattacttag tgtttctagt attatagtat taagttcatt attcatcata tgttacctcc
      541 ataatatata taatacacta aattagaaag taaaacaata ttaaggcttg taaaaaaaac
      601 ataaagtggt ataattaaat atataatata gtaatggaaa ctatatttgt gaatctgatt
      661 tttaaaatac ataattgtag gaggacgaaa tgtatcaatt ttttttaaag ggtagagacc
      721 caataagcag tttaactcat tttataggtg cttgtttatc tcttttagca actataattt
      781 tagtttttca atctgttaca ttacaggaaa cttctctctt gatgatagta tctgtatctg
      841 tatttggact ttcactaatt gctttatata gtgcaagttc gtattatcat tttttaaagg
      901 ggacaccaga acaagaattg ttttttagaa aagtagacca tgcaatgatt tacgtattga
      961 ttgctggctc ttatacacca atatgtctga attttatgga aaaaaaagaa ggcataatat
     1021 ttgttacagc aatatggata gttgcattta ttggcataat aataaaaata ttttggatgg
     1081 atgcacctag atggttatct accagtatat atttacttat ggggtgggca attgtttttg
     1141 atataaatgc attcaattca attcctaagg attgccttag actattaatt atggaaggtg
     1201 tatcttactc tataggtgcg attatttata taattaaaaa gcctaatata agtcctgagt
     1261 ttggatttca tgaaatattt catattttta ttatgatagg ttctttattt cactttttag
     1321 cagttttatt gtatgtttta tagttttaaa agatttgatt aattgatact aaaattctac
     1381 aatgaataaa atggaactgc cttatataga ttatatctta gacaataaga cagttc
//
LOCUS       DAELJO010000397         1425 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.400, whole genome shotgun sequence.
ACCESSION   DAELJO010000397 DAELJO010000000
VERSION     DAELJO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.400"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(234..701)
                     /locus_tag="KPB88_003998"
     CDS             complement(234..701)
                     /locus_tag="KPB88_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG0056202.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(780..1322)
                     /locus_tag="KPB88_003999"
     CDS             complement(780..1322)
                     /locus_tag="KPB88_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056203.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT          466 a          255 c          113 g          591 t
ORIGIN      
        1 tcaataattt ctaattattt ttttaacaat atttattaca ttttttccac ttttttgtca
       61 tttttttatt gtttcgaaaa aaaatcagaa ttttttaact ttattttgga ttattagttt
      121 aaattagatt ttcctcttag atttataaat atttacacaa aaaaactacc tctgtataga
      181 ttgtatacag aggtagtttt ttactaattc ctactatagt ttactatatt tttttaccaa
      241 ttcttacaaa catctaccca tttttcacac ttagaaaact tctccatttc ttcacatgta
      301 agttctattg cagagttact actgccacat gcaggaaata ctgtatcaaa tcttttcatt
      361 gaatcatcta aatagacttt tatagaattt tttaaaccaa atggacagac tccaccaata
      421 gcatgtccag taaactccaa gacttcttct ggtgtcaaca ttttagcctt acaattaaat
      481 tcagccttat acttcttatt atcaatctta gcatcaccag ctgtcacaat taaaattgca
      541 tcatctccta ttttaaatga gagtgtctta gctattcttg ctggaataac cccagctgct
      601 tcagctgcaa gttccacagt agcactagat tgttcaaatt ctaatatact gtcttccttg
      661 ccaaattgtt taaaatactc tcttacatta cttattgaca ttaaataccc ctcctagtct
      721 gaacttttat aaatttcata ttattcttaa ttatatctat aattgattta tttttcaaat
      781 tattccataa tattagcatt ccatgtatca ttattttttc caactactag ataaagtcct
      841 tttgccattg gaatattgac aatatattct cctttatact ctacactcat accagattca
      901 ttaaagtacc tataatcaaa atttagtcct tttggaatta caaaatcaac ataaccttga
      961 ttatttggtt ttattccata tttcagagtt ctttcagtta tagaattttt ttcattaaat
     1021 aaatctacat ttacatatgc agaacactta ttcattccct ttttattttc cacacaattt
     1081 tcaaacttca ttttagcact gtttaattta taactatatt tactattatt gcttatttta
     1141 aatcttacaa tagtaccaac atctctactt ctcctgctat caaaatcaaa aacaatttcc
     1201 tctaaatgat ttatttcata atctttgaca cttaccatat ctctatatac taatttattt
     1261 atagctatga atagtgtaaa aatccccaaa ataaaaaatg aaacttttat aaacttcttc
     1321 atttttagtc tccttatatt ttgtgctatt gtgctataat attaatcaaa attttaataa
     1381 ttaaacttat tcttttttga aatcttaata gaaaaataca ttgat
//
LOCUS       DAELJO010000398         1385 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.401, whole genome shotgun sequence.
ACCESSION   DAELJO010000398 DAELJO010000000
VERSION     DAELJO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.401"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            123..239
                     /gene="rrf"
                     /locus_tag="KPB88_004000"
     rRNA            123..239
                     /gene="rrf"
                     /locus_tag="KPB88_004000"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            247..321
                     /locus_tag="KPB88_004001"
     tRNA            247..321
                     /locus_tag="KPB88_004001"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Asn,seq:gtt)
     gene            327..412
                     /locus_tag="KPB88_004002"
     tRNA            327..412
                     /locus_tag="KPB88_004002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Leu,seq:taa)
     gene            428..503
                     /locus_tag="KPB88_004003"
     tRNA            428..503
                     /locus_tag="KPB88_004003"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:461..463,aa:Met,seq:cat)
     gene            511..585
                     /locus_tag="KPB88_004004"
     tRNA            511..585
                     /locus_tag="KPB88_004004"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:544..546,aa:Glu,seq:ttc)
     gene            600..673
                     /locus_tag="KPB88_004005"
     tRNA            600..673
                     /locus_tag="KPB88_004005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:632..634,aa:Gly,seq:tcc)
     gene            679..754
                     /locus_tag="KPB88_004006"
     tRNA            679..754
                     /locus_tag="KPB88_004006"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:712..714,aa:Val,seq:tac)
     gene            760..836
                     /locus_tag="KPB88_004007"
     tRNA            760..836
                     /locus_tag="KPB88_004007"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:794..796,aa:Asp,seq:gtc)
     gene            847..921
                     /locus_tag="KPB88_004008"
     tRNA            847..921
                     /locus_tag="KPB88_004008"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:879..881,aa:Gly,seq:gcc)
     gene            931..1004
                     /locus_tag="KPB88_004009"
     tRNA            931..1004
                     /locus_tag="KPB88_004009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:965..967,aa:Arg,seq:tct)
BASE COUNT          413 a          270 c          313 g          389 t
ORIGIN      
        1 atttgatgtt cattcattaa gatatatgtg tagtttttag agtgctaact ctaaagaaac
       61 tagtattgct aggttctata aacttattaa aaacttaata gaaatattaa gcataaagat
      121 tatgtggtta caatagcaga gaggatacac ctgttcccat tccgaacaca gaagttaagc
      181 tctctagcgc tgatggtact tggtgggaaa ctgcctggga gagtaggacg tagccacgtt
      241 aaatgttcca aggtagctca acggtggagc aaccggctgt taaccggtag gctgtgggtt
      301 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
      361 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      421 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      481 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      541 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      601 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      661 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      721 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      781 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      841 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      901 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      961 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
     1021 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
     1081 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
     1141 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
     1201 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
     1261 tattgtatac tttatggtat ttatatattg aaataactta tgaagttatt tataaagaga
     1321 ttcatgagca tatttttgtc taaacaataa gtataagaaa ttataaaaaa ttagagacta
     1381 tagtg
//
LOCUS       DAELJO010000399         1379 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.402, whole genome shotgun sequence.
ACCESSION   DAELJO010000399 DAELJO010000000
VERSION     DAELJO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.402"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            132..275
                     /locus_tag="KPB88_004010"
     CDS             132..275
                     /locus_tag="KPB88_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056204.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     regulatory      complement(477..574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            800..1183
                     /locus_tag="KPB88_004011"
                     /pseudo
     CDS             800..1183
                     /locus_tag="KPB88_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          571 a          144 c          208 g          456 t
ORIGIN      
        1 tggagaatag atatttgata attgtaaaat tatgttataa ttaaaatgca agaataatct
       61 tgtggaacta caatctaaga gtggagcttc attttctaca ttccatccct taaaaggaag
      121 gaggtggaga aatgagcgaa tttttactag gagtgttagc tagtttaaca gctagcttta
      181 ttacatatat tatttccaga aaagtaaaaa gccactctgg caggagtgac tttgagcttg
      241 atgtaaaaat caagtttaat aaaaaacgac attaatattt agttaatgaa acttcactct
      301 aggctaatag attgtagttt ctttttttgt ttttgatacg aatttacatc tttattatat
      361 cgcattttaa gaaaaaataa aactgtgaat gctagaaata tattttattt tatctctaag
      421 tatatatatt attatccccc taaaaaactt tcataaaaac aaaagaacaa tatctctttg
      481 gcaactggct gacataactc ataagatatt tatataagtc ttagtcccta tagctttgcg
      541 tcactaaatt tctctagttt tgccgattta gttttattct acaactaaaa taatacaata
      601 gaattagtta ttattcaaca ggattgtttg aaagttaaat aaatttatag atatgaatat
      661 tattttacac ttaaaaacag actaaacacg tagagtacct ggtaaaaatt aaatatagtt
      721 tttgttctaa aaagctccta ttataattta agctagataa atttgaagaa gtgacataaa
      781 ttgaaagaag gaatatatta tgctaaataa aaaattacca gatacagaat taaaaattat
      841 gaaatgcata tggaatactg gttataaaac tgtaatatca aaagatgttg cagatgaaat
      901 agaaaaaaca tatggttgga agcatacaac tacaattata cttttagcta gattaaccaa
      961 aaaaggtttt ttaatatcat agagaattgg gaggcatgta cactatacag tattaataaa
     1021 agagagagaa tatctcaaaa tagagggaaa aagagttttt agaggtttac ataacaatcc
     1081 tttatcagaa ttaatttcaa aattacatga tggagaagaa ataactgaag aaaaaataat
     1141 agaaatagga agttggataa aaagctggaa atataaagat taataaataa aaggtagcta
     1201 ataatataag tgctaccttt tttatgtgga taatattggt gataagttgt ggaaaactta
     1261 tataaaaatt gtggataaag tgttttgctg acattttact gacaaaagtt aaaaaaataa
     1321 atgttatata tattgtaaaa tgttgaaaaa tagccatata tataaaatat aagtaacac
//
LOCUS       DAELJO010000400         1337 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.403, whole genome shotgun sequence.
ACCESSION   DAELJO010000400 DAELJO010000000
VERSION     DAELJO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.403"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            27..101
                     /locus_tag="KPB88_004012"
     tRNA            27..101
                     /locus_tag="KPB88_004012"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPB88_004013"
     tRNA            116..200
                     /locus_tag="KPB88_004013"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPB88_004014"
     tRNA            210..293
                     /locus_tag="KPB88_004014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPB88_004015"
     tRNA            323..399
                     /locus_tag="KPB88_004015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPB88_004016"
     tRNA            407..482
                     /locus_tag="KPB88_004016"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="KPB88_004017"
     tRNA            571..659
                     /locus_tag="KPB88_004017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="KPB88_004018"
     tRNA            663..738
                     /locus_tag="KPB88_004018"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="KPB88_004019"
     tRNA            745..818
                     /locus_tag="KPB88_004019"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="KPB88_004020"
     tRNA            833..909
                     /locus_tag="KPB88_004020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="KPB88_004021"
     tRNA            933..1009
                     /locus_tag="KPB88_004021"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="KPB88_004022"
     tRNA            1017..1093
                     /locus_tag="KPB88_004022"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="KPB88_004023"
     tRNA            1102..1177
                     /locus_tag="KPB88_004023"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="KPB88_004024"
     tRNA            1185..1258
                     /locus_tag="KPB88_004024"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="KPB88_004025"
     tRNA            1265..1336
                     /locus_tag="KPB88_004025"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAELJO010000401         1331 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.404, whole genome shotgun sequence.
ACCESSION   DAELJO010000401 DAELJO010000000
VERSION     DAELJO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.404"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            102..809
                     /locus_tag="KPB88_004026"
     CDS             102..809
                     /locus_tag="KPB88_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG0056205.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(951..>1331)
                     /locus_tag="KPB88_004027"
     CDS             complement(951..>1331)
                     /locus_tag="KPB88_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0056206.1"
                     /translation="QEESNLNSIVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNIN
                     FIKIGHHGSNSSSSLEFIKKISPDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTD
                     RIGDIVFYSDGEIIFTKYNYEIDT"
BASE COUNT          521 a          139 c          194 g          477 t
ORIGIN      
        1 taagataatt tatttttaat aatatataat gttttgtatt atataatgta aattttctaa
       61 tactattata tatttagata aaaatctagg agtgtggaaa gatggaaaat aattttacaa
      121 cgagaactag ttttctagtt ggagatgatg ggataaaaaa acttaataat tcaaatataa
      181 tagtatttgg agttggagga gttggtagtt ttactgttga agctttagca agagctggag
      241 taggcaatat tactattgta gattttgatg atgtagatat aaccaatata aatagacaaa
      301 taccagccct acattcaact gtaggaagat acaaagttga tgtcatggaa gaaagaatat
      361 tagatataaa tcctaatatc aatataaaaa aaataagaag tctttataat aaagatacta
      421 gtgacgaaat actaactgaa agatatgatt atgttgtgga tgcgatagac atggtaagtt
      481 ccaaaataca tctaattgaa acttgtgaaa aaaaaggatt gaaaataata agttctatgg
      541 gtatgggaaa taaattagac ccaactaaaa tagtcgttac agatatacac aaaacaagta
      601 cttgtcctct agctaaagtt atgaggaaag aacttaggga tagaggtatc aaaaagttaa
      661 aagtagtata ttctactgaa caaccaatag aacttaagaa gaaagtaatg aatggtagaa
      721 aagtaacacc tggtagtgta tcatttgtcc catctgtagg aggtttaata atcgcatctg
      781 taatagtaaa tgaactatta ggacaataag ttttaaatat tttaatttat aaattttata
      841 tacaaatttg aaggctagaa gcatgaatac aaagcaattt tatattcata cttttagcct
      901 aattatttta tattacctta ttttatgtag ttgtagtttt attttttaga ttatgtatct
      961 atttcataat tatattttgt aaatataatt tctccatcac tataaaaaac aatatctcct
     1021 attctatctg ttctataaat tgatacatgg ttttgtttta gattattaat aacctcccta
     1081 tgtggatgac catattgatt tttatatcca catgatattg cagctatatc tggactaatt
     1141 ttttttataa attctaaaga tgaagaagaa ttacttccat gatgacctat ttttataaaa
     1201 ttaatattat ttaatttaaa agaatgtaaa atttcttttt cattttcttc ttcagcatca
     1261 cccataaata aaaaagaatt atcattaaaa gttaatttga aaactataga atttaaatta
     1321 gattcttctt g
//
LOCUS       DAELJO010000402         1307 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.405, whole genome shotgun sequence.
ACCESSION   DAELJO010000402 DAELJO010000000
VERSION     DAELJO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.405"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            95..>1307
                     /locus_tag="KPB88_004028"
     CDS             95..>1307
                     /locus_tag="KPB88_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0056207.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVNLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVD"
BASE COUNT          603 a          108 c          231 g          365 t
ORIGIN      
        1 ctaataaata atataaaaat agtagaacct tgtattataa taatataatg tagaaaaata
       61 tatagataag ataaatataa taaggtggta atatatgaaa tatacatttg aaaaatttaa
      121 gttaaatgaa aagatactta agtcattgaa gagtttagga tataatatac cttctagagt
      181 acaaagagaa gttattccaa aattattaaa aggtcaaaac ttagtagtta ggtcaaaaac
      241 aggaagtgga aagacagcaa gttttgcaat acctttatgc gagaatataa atgtagatta
      301 caataatatt caagctctta tagtggttcc aacaagagaa ttagccttgc aagtaaaaga
      361 tgagatatca gatataggga gactaaagaa ggtgagatgt agtgctatat ttggcaaaca
      421 atctataaaa gaccaaatag ctgaattgaa acaaagggtt catattgttg tagctactcc
      481 aggcagaata cttgaccata taaatagagg ttctattaag ttggaaaatg taaagtattt
      541 agtaatagat gaggcagata aaatgtttaa taaaggtttt gttgaacaaa tggaaaaaat
      601 attattaaat ttacctaagg agaaaatagt aaatcttttt tcagctacaa tagatgaaga
      661 gataaaatat atatgtgaaa aatatatgct agattacagt gttataaata tagaagaaaa
      721 tgagagcgat actaatcaaa aaacaagaca aatagatgac aaaataatca aggcaaatgg
      781 aagagagaag tacatccttt taaaagaatt aatatacagc gaaaatccaa agagtgtgat
      841 aatattttgt aataccaaag agaaagtatc taaattatat aataagatga gtaaagaggg
      901 ttttttaata agagagttac atgcagattt atcacaagaa agaagaattt ttgtaattaa
      961 ggattttaaa aaccaaaaat ttaatatatt agttagtagt gatgtagcgt ccagaggaat
     1021 acatattgat gatatttctt tagtaataaa ttatgatgtg ccacaagata aagaaaacta
     1081 tatacataga ataggtagaa ctgggagaaa aggaaatagt ggaaaggcaa ttactatagt
     1141 aactgaaaag gatgaaaaat atatagagaa tatagaaacg tatataggat ataaaataaa
     1201 tgaattaaaa gatatagaaa aaagtagaat tacacatggc aaaattttgt ttgaagaata
     1261 ttcaaaagga atcctaaaaa aagtatcaaa gagaaaaaaa gttgata
//
LOCUS       DAELJO010000403         1284 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.406, whole genome shotgun sequence.
ACCESSION   DAELJO010000403 DAELJO010000000
VERSION     DAELJO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.406"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            106..>1284
                     /locus_tag="KPB88_004029"
     CDS             106..>1284
                     /locus_tag="KPB88_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG0056208.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNKFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     D"
BASE COUNT          547 a          161 c          150 g          426 t
ORIGIN      
        1 aaattttttt acgtttttta attttataat taaaaattta aattttatac atattataat
       61 aaattgtgat atatataaaa taattaaaga taaaaggagt attttatgaa catacttgga
      121 ttaatagttg aatataaccc atttcataat ggacacatat atcatttatc taaatctata
      181 gaaaaaacaa atgcaacaca tactgtagca ataatgagtg gaaattttct acaaagaggc
      241 gaaccagctt tatttgataa atacactaga gcaaaaattg ctgtcgaaaa tggtgtagac
      301 ttagttatag aattaccaac tttatttgct tgtcaaagtg ctgaaatttt ttctcatggt
      361 gctataacaa ttttaaattc actaaactgc ataaattcta tatgttttgg tagtgaagaa
      421 ggaaatacag aaattcttta taatatagct gaaattttag caaatgaacc aaataaattt
      481 aagttacttt taaaaaaata tttagatgat ggtctgttat ttccaacagc tagagctaat
      541 gcactttttg attatataca tcaagacaaa aaaaataatt ttatacaaaa ttattcaaag
      601 gaacaattat caaaaacatt aaattcatca aacaacatac tgggtataga atacataaaa
      661 aacctcatcc atttaaacag tagtataaaa ccatttacta tctccagggt ccaatcagaa
      721 tataattccg aagaaattga gagtgatatt tgttctgcta ctgctataag aacttcactt
      781 aaagaaaata cagacctttt tcatttaaaa aatgttattc cagaaaaaac ttataacatc
      841 ctaaatgaaa aaatagataa tggtttttta cctatgtttg atgacttctt ctttgatatt
      901 ttaaaaacaa ttatacttag agatatggac actttaaaag agtattttga agtaaatgaa
      961 ggcatagaaa acagaatcta taaagatatc tttacatctg aatttctaca tgaactacaa
     1021 ttagcaatta aatccaagcg atacacttta accaaaatca aacgaactct aaacaatata
     1081 ttgttaggta ttaaaaaaag cgacatgcaa cttataaaaa acatagatac tataccttat
     1141 ataagaatac ttgcttttaa tgataaaggt agagaaattc taaaagagat aaaaaataaa
     1201 tctgaaataa aaataataaa caagttttca aatatatcat tttcattaga tgatacgatt
     1261 tttaaaacac tcataagtta tgat
//
LOCUS       DAELJO010000404         1261 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.407, whole genome shotgun sequence.
ACCESSION   DAELJO010000404 DAELJO010000000
VERSION     DAELJO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.407"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..240
                     /locus_tag="KPB88_004030"
     CDS             <1..240
                     /locus_tag="KPB88_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056209.1"
                     /translation="KNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICSPVGI
                     IYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            530..1072
                     /locus_tag="KPB88_004031"
     CDS             530..1072
                     /locus_tag="KPB88_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0056210.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          555 a           96 c          187 g          423 t
ORIGIN      
        1 aaaaatgata tacaaaataa caatgaaaat ataaacgatt gtaaaaagca aataagtttt
       61 ggtgataata aggtaaaaaa aataacaaaa acttttggat tttcttgttt catttgttct
      121 ccagttggaa ttatttatga agatatgaaa aatataaata taaaaattga acatacatca
      181 ataaatgaat taaatccaga agaattattt gtaaatacat cacttttatt gtattattaa
      241 gtattttaaa gctaaaactt aaacttatta aaatagcatt tggttaaagg aaaatagata
      301 ttgatatatt tatcatatat ataaatgatt aattaaacaa ttataaataa taagtatatt
      361 ataatattta aaaaatatag atttatgata aaataagaca aatatgttaa aaaaatgata
      421 atcacggaga acttttacct tcttcaatta atatattatt attagtaata actttaaaat
      481 tatattacta ttatataaaa attggcttaa aagaaggtga aaaaagtaaa tgaaaatatg
      541 cataacagta ggtcatagta tactaaaagg tggtaaaagt acaggtgtaa atggtatagt
      601 tgatgaatac aattataata aaaaattagc tcctatgtta gctgagatgc ttataagtca
      661 aggtaataca gtagatgtaa taatttgtcc agaaaggcac tttatgtcag aaagagaaga
      721 atttttctat agagttccaa aagtaaacag tggtaaatac gatttattag tagagctaca
      781 tttaaataaa gctgatggaa cacaatgtgg tacagaagtg ctttactatg gcactgaagg
      841 attagaatat gctaaaagag tatctaatag acttggagaa ttatttgaaa atagaggagc
      901 taaaaaaaga gaaaatttat atatattgag aaatacaaat ccagttgcaa tacagattga
      961 aagttttttc tgtgataatg tgaatgattg taacaaagca aatgaatcag gttatgatta
     1021 tatagcacga ttgattacag aaggtatatt gaataaagat ataaatatgt gagattatgg
     1081 tgatttaggt attgattaat accttttaat attagatata aataaaaata caatgtatta
     1141 gtataattta aactttcaaa agatagatta gtttgattat tgacaagaga tacctccttg
     1201 aaaatattga tttatcagta tatttaagga ggtatttaaa tataaaaatt taattaattt
     1261 g
//
LOCUS       DAELJO010000405         1248 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.408, whole genome shotgun sequence.
ACCESSION   DAELJO010000405 DAELJO010000000
VERSION     DAELJO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.408"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            123..380
                     /locus_tag="KPB88_004032"
     CDS             123..380
                     /locus_tag="KPB88_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056211.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(490..1170)
                     /locus_tag="KPB88_004033"
     CDS             complement(490..1170)
                     /locus_tag="KPB88_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0056212.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          518 a          151 c          138 g          441 t
ORIGIN      
        1 tgatataaat aatcgtatta aaaaataaaa tactaagttt aaaaatttaa ataatctata
       61 aatcaattat aaaatattat ttcttagaaa ttaaaatgaa ttaagtaaat ggaggaattg
      121 atatgataat aattagaagt caagataaaa ctaatttaat gcagataaac caaattaaaa
      181 tagatggtag tcaagtttat gcagtatttg aaagtaaaat tgatactgta aaaataggag
      241 attatgaaaa taatacaaga gctatacaag tgttggacaa tatacaaaac ttcattgaaa
      301 atggaacaaa atatgattat ataacatcaa ataaagtcag atacaatgtt aataaaatat
      361 ttcagatgcc agctaaatag tgagtgtgca ttttagttta ataactaaag cccactttct
      421 tttgtagtat gaaagagggc tttacatatt tgtaaatatc gaagtacact tgttttatca
      481 ataaattatt taataaggta taatggacca attaaacaat aaaaattctc ctaaaacagc
      541 aagtattaaa tatataatag cttcattttt gtaaagagag gtaatttttt cgcttatgtc
      601 atcatcaatt gtaatagcaa tttcatcttg aataatattt ttatatatag atgggaaaat
      661 atcttttttc tttataagat taaataaatt atttacacct acccatatca aatatcctaa
      721 aacagcacct aatgtgttca tcattaaatc atcaatatct gtagctctta agttaaacaa
      781 ttgacaaagt tcaattgcta atgaaaaacc taatccagtt aatgaaacat tgataatatt
      841 tctaaaattt ttccaaatta aaggtagtaa aaatcctaaa ggcataaaca taattatatt
      901 taaaatatat gttaagatgc caactgagtc aaaaggtatt agatttattt catcaattct
      961 aaaaagccca ctataccttc ctatatccca tatgctacca attccagctt tactaagtgc
     1021 tagatagata tataataaaa aaatatatac ccatactaaa tgtatcatta cattcttttc
     1081 aactttcaaa ttttttcgtt ttataacgac tatttggtat attaagcatg gtaatataac
     1141 gcaaataaat gaataaaatg tccatatcat tttattttgt cccctttcaa atttatgttt
     1201 ttagttttat aagtactatc tttatattaa actgtaagtt ttaaaaat
//
LOCUS       DAELJO010000406         1184 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.409, whole genome shotgun sequence.
ACCESSION   DAELJO010000406 DAELJO010000000
VERSION     DAELJO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.409"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..351
                     /locus_tag="KPB88_004034"
     CDS             <1..351
                     /locus_tag="KPB88_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0056213.1"
                     /translation="IYRNFIRIPFGEIKGKLEYLCKIKNINFIRQEESYTSKSDFFAD
                     DFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVNASLNILKKSQVVNLSNLQN
                     NPQILKQPQRVRIS"
     gene            534..>1184
                     /gene="yqeC"
                     /locus_tag="KPB88_004035"
     CDS             534..>1184
                     /gene="yqeC"
                     /locus_tag="KPB88_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG0056214.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIEN"
BASE COUNT          514 a          115 c          185 g          370 t
ORIGIN      
        1 atatatagaa attttataag aatcccattt ggagagataa aaggaaaatt agaatatctg
       61 tgtaaaataa aaaatatcaa ttttattagg caagaagaaa gctatactag taaatcagat
      121 ttttttgctg atgattttct acctgatatt aatgtggaca atgttaaaaa atatgacttt
      181 aaaggggaaa gaataacgcg tggacagtac aaatccagtg ttggaatcat tataaatgca
      241 gatgtaaatg cgagtcttaa tatactaaaa aaatcacaag tagttaattt atctaattta
      301 cagaataatc ctcaaatatt aaagcaacca cagagagtaa gaatatctta attatattta
      361 aaagataaac tgatagcgta agcttacagc ttagcggcga attggatccg ccttgttgtt
      421 tacaacagaa tctatttact ttcgagttta tggaatttca aaaatcactt ttactaaaaa
      481 ggtctaacta taataattaa gagattagaa aactggttat aagaggtgaa agtgtggaac
      541 tatcaaatat aatagaaaag aatgaaataa taactgtagt tggagcagga ggaaaaactt
      601 cgtttataaa ctattttgca aatttttata gagataaatt gaaagttctt cttacaacaa
      661 caactaaaat atatgtacca aacgattatg acaatataat tattacaata gatggaactg
      721 ttattccatc tatttgtcat ggaataacag tatgtggcag ttatataaat aatgaaaata
      781 aattagtaag tattgattcc tctattttgg atgaaattgt agaccaattt gatttagttt
      841 taatagaggg agatggctca aagagaaaaa aacttaaagg ttggaatgct aaagaaccag
      901 tagtatatca taaaactaca aaaaccatag ggatattaga tataacatca tttggaatga
      961 atattaatga agagaatata catagagtag aaatatttaa aaaaatagca aatttagata
     1021 caagtagtat taatagtagt tctacagtaa gtattgaaaa cttaaaaaat atagtattaa
     1081 atccaaatgg attatttaaa aattattctg gaaaaagagt attatttatt aataaagttg
     1141 aaaatgaaaa atataaaaat ttagctataa aattaataga aaat
//
LOCUS       DAELJO010000407         1153 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.410, whole genome shotgun sequence.
ACCESSION   DAELJO010000407 DAELJO010000000
VERSION     DAELJO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.410"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            199..1125
                     /locus_tag="KPB88_004036"
     CDS             199..1125
                     /locus_tag="KPB88_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0056215.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
BASE COUNT          433 a          150 c          211 g          359 t
ORIGIN      
        1 gaaaaaatat tataagttgt taaatttaaa tatctaaact caattatatc tgtacaattg
       61 taatttttta aatcataatg tattatttta aatatacagt tacattatta ttcacaatat
      121 aagtatctta gttctcaaat aacatctcat gtaatctatt caatgcaaaa aatttaaata
      181 ttttttagga ggttttttat gagcaaggaa aataaaaatg taagaattgc tgttgtccag
      241 gcgtcaccag tcataatgga tttagaaaaa actgttgaaa aagcattagg attaattaaa
      301 gaagctggta gaaaaggggc aaatatcgta gttttccctg aagcattcat acctgcatat
      361 ccaagaggtc tttcatttgg atttgttgtt ggaagtagaa caatggaagg tagagaagat
      421 tggaaaagat actatgataa ttctgtacca gttcctagtg ctacaacaga tttattagga
      481 aaagcagcac aagaagcagg agtatatcta tcaatgggta taacagaaag agatggtaat
      541 gatataaatt gtacccttta ttgcacaaac ttatttttct ctccagaagg taagctaatt
      601 ggtaaacaca gaaaacttaa acctacagga acagaaagat gtatttgggg agaaggagat
      661 ggaagtactc ttacagtagt tgatactcca tatggaaaaa tgggttcgtt gatttgttgg
      721 gaaaattata tgccattagc tagaacagct ctttatgcta agggtgtaaa actttacata
      781 gcccctactg cagattcaag agaagagtgg caagcaacta tgaaacatat tgctttagaa
      841 ggaagatgtt ttgtaattgg atgcaatcaa tatgttgaaa aaaatatgta tccaactgac
      901 cttaactact ataaagagct agatgcagaa ccagaaatta tgtgtccagg aggaagttgt
      961 atagttgacc cttttggaaa atatgtagca ggtcctattt tcaataaaga agaaatgcta
     1021 atagctgatt tagacctaga aaaaatagta cttagtagac tagattttga ttcagaaggt
     1081 cactattcaa gaccagatgt atttgaatta attgttcatg aataatcttt ttacacaaac
     1141 atctacataa atc
//
LOCUS       DAELJO010000408         1142 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.411, whole genome shotgun sequence.
ACCESSION   DAELJO010000408 DAELJO010000000
VERSION     DAELJO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.411"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(286..1032)
                     /gene="cdtR"
                     /locus_tag="KPB88_004037"
     CDS             complement(286..1032)
                     /gene="cdtR"
                     /locus_tag="KPB88_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG0056216.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIMFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          427 a          141 c          120 g          454 t
ORIGIN      
        1 aggtatatat cataaaaata tgtatttata tgatatgtca gtacaaaata acaactgaat
       61 attttaaaga gactatttcg tacttttatg atttaatgag ctatcttatc gaatcataat
      121 ctaggaacaa ttgttataat atctaatagt agttatatat gatatagtca gtatgtgttt
      181 tttcacaata tgattattat atagaggatt cttagggatt accctttttg tataaaaaag
      241 agatggttca aatttttatc ttgagacatc tcctttttct atttattatg ttttaataat
      301 attctttaaa atatttctat atgtattaga tatacgacat atatggccat tactcatttt
      361 aatattattt ttctttaggt cgagagaaac aatatgatat ttattaacta caaaagattt
      421 atgtacacgg ataaagcatg catctaaatc tggtaaagac tttaaacttt tatagaattc
      481 aaatattcta gaagtagtca ctaattttat tttatgtgga atagtgcttg tttcaaaata
      541 tataacttca tcaaatgaaa ttttccatag tgagaaatct gaatagatag taaaaaaatt
      601 agagcgataa tttttttctt taatgtatct tttgtgagca attttaatac aagcttttat
      661 tttactttca gcaatttcca tatcatgttt tagtataaag tccataactt ctaacctata
      721 atcgaagatc cctaaaatca tttctatatg atttgtaaga aacatgattg gactaatata
      781 atctgattct ctaatttttt gtgctatcca taatccatca cataattcat tacttatctt
      841 atattttgcg tctacaacta taaaatatat tcttacttta tttttttcag taagctcttt
      901 taataacaaa tttgcattat gatataatct tacaacatca ttatcaaaac cttcttttat
      961 taaaatatta tttatttttt cttttagttt cattccaaaa caaacatcat tatcaaagat
     1021 taatatatcc actaaatacc ctcctataaa aaattcaaaa gtaaaaaaac aaaagtattt
     1081 tatagaaaat acataaaata acactaggag ataaataaga ttaaggatag ttagaattaa
     1141 gg
//
LOCUS       DAELJO010000409         1132 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.412, whole genome shotgun sequence.
ACCESSION   DAELJO010000409 DAELJO010000000
VERSION     DAELJO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.412"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            206..1117
                     /locus_tag="KPB88_004038"
     CDS             206..1117
                     /locus_tag="KPB88_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0056217.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
BASE COUNT          488 a           83 c          202 g          359 t
ORIGIN      
        1 aattgatata gaaataaata aattaagatg ataatatcca aagtgagaaa gataatagaa
       61 agtaaataaa atcatgaaag ataatagaaa gtaaataaaa tcatgtaaga ataatttaga
      121 taagattcta agattttatt tatttaaaaa ttagtatgat taatttagat taaataaatt
      181 taataatttt agtaggtggt aatgaatgaa aataaataga ttaacagaaa taatagtaat
      241 tctattaaat aaaaaattag taacagctaa agagttggca gataggtttg aagtttctac
      301 aagaaccata tatagagata tagaaactct gtctatgtcg ggagtaccag tatatatgac
      361 gaaaggcaaa ggaggaggga tatctttaat agaagaatat tctatagaca aggccatatt
      421 gtcaaaaaaa gataaagaga gtttaatagt tgcactgaaa acattacagg cgactaagta
      481 tccagaaata aattcagtag taaataaaat tggctcaata tttggagagc aaaatttttc
      541 aaactggatt gaaatagact ttactgaatg gggaagtaat tttaatgaag atgataaatt
      601 tacaaaaata aaagaagcta ttttaaggcg taatactata aattttaatt atgtaaatag
      661 tttgtcaagc cagacaaaca gaactgtcga accactgaag cttatgtata agagtaagac
      721 ttggtacttg tatggatttt gtaaactaaa agatgatttt aggatattta gaattagtag
      781 gataaggaat ttatctataa aggatgaagt attttcaaga aagataatag aagaagtatg
      841 tttgaatgat tctaaagtaa ttaaggaaaa tactataacg ttgaaattga ggtttaaaga
      901 aaagatgctt tttagagtat ttgatgattt taataaggat ttaataacta aaaatgaaga
      961 tgatacatat gatgtaatta cagaatttcc tattggtgaa tggatttatg gatatatttt
     1021 atcatttggt gacaatgttg aagtacttga acctaaagat gttagggata atgtaataac
     1081 tagactaagg gagttgtcta aaatttattc attataatat aatgctagat ta
//
LOCUS       DAELJO010000410         1124 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.413, whole genome shotgun sequence.
ACCESSION   DAELJO010000410 DAELJO010000000
VERSION     DAELJO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.413"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            188..922
                     /locus_tag="KPB88_004039"
     CDS             188..922
                     /locus_tag="KPB88_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0056218.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT          466 a          116 c          169 g          373 t
ORIGIN      
        1 ttacttctat attaattact ataccataaa ataacaaaaa aatcaagtcg agtaatcctt
       61 caacttaagt gatatactag gtattaataa tttttaatta ataaaatata actagaggaa
      121 aatctacatt ttctcaattt ctttaattaa attgacctct aattaagtaa ttataaggag
      181 aaaaattatg aatttagcaa agggattaga tgttttaaaa atttcatcaa atgtattcgg
      241 tgaagataaa gttatgtata ttcctgtaat ctacacagaa gatgatgcaa cacttattga
      301 cacaggtctt ccgggtcaag gtgatttaat aattgatgct cttaataaaa gtaacacgag
      361 ctttgataag ttaaaaaata taataatcac tcatcatgat atagaccata taggaaacat
      421 aaattatcta agagaaaaat ctaagaataa tatcaaagta tatgcataca aaagtgaaat
      481 cagctatata acaggagaag aaactccttt taaactttat atgttggaac aaatggtaga
      541 taaaattgat gataagatgc ttagtatgct aaatgttatg ggacttggat ttaaatcatc
      601 atatactaaa gtagatgtgt cattagataa ccatgaaaaa ttgaatctag gtgaggaaat
      661 agaagttata catacaggag gtcacactag aggtcacata tgtctttatt taaaagagtc
      721 taaagtactt attgctggag atttgttaca ggtagaaaat ggagaactta agcctgttga
      781 tgtgatgtac agcaacaaac aagagttaaa agatgctatt aaaaatataa gtaattatga
      841 tatagaaacg atagttttta gtcatggtgg attatatcaa agaaacataa ttggaacatt
      901 gaaaaaccta attattgagt agtactatat gatttacaat aatttcttaa attacttaaa
      961 attatatcta aagatgtaga atcaaaatta aatataagta ataagaattt tgctttaaaa
     1021 ttatacttaa gtgttaaggg tgatacgtag tattacaaat attttagaat ataatttttt
     1081 atagtaattt taaggttcta caatacttca gttgaaaaat attt
//
LOCUS       DAELJO010000411         1112 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.414, whole genome shotgun sequence.
ACCESSION   DAELJO010000411 DAELJO010000000
VERSION     DAELJO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.414"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>1112
                     /locus_tag="KPB88_004040"
     rRNA            <1..>1112
                     /locus_tag="KPB88_004040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          249 c          332 g          238 t
ORIGIN      
        1 ttagtagcga agtagctgat ttcacactgt caagaaaagt ctctatcgag gttaaaggta
       61 cccgtaccgc aaaccgacac aggtgggtga ggagagtatc ctaaggccag cgagagaact
      121 gttgttaagg aactcggcaa aatgaccccg taacttaggg ataaggggtg ccaccatcag
      181 gtggccgcag agaataggcc caagcgactg tttaccaaaa acataggttt ctgctaagtc
      241 gcaagacgat gtataggagc tgacgcctgc ccggtgctgg aaggttaagg ggatctgtta
      301 gagcaatcga agcagtgaac ttaagcccca gtaaacggcg gccgtaacta taacggtcct
      361 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt aacgatttgg
      421 gcactgtctc aacaacagac tcggtgaaat tgtaattccg gtgaagatgc cggatacctg
      481 cgacaggacg gaaagacccc atggagcttt actgtagctt gacattgggt cttggtacta
      541 catgtacagg ataggtggga ggctttgaaa ccaggacgcc agttttggcg gagccatcct
      601 tgggatacca cccttgtagt actgggactc taaccatagg ccatgaatct ggtcttggga
      661 cactgtcagg tgggcagttt gactggggcg gtcgcctccc aaaaggtaac ggaggcgctc
      721 aaaggttctc tcagtacggt cggaaatcgt acgtagagtg taaaggcaaa agagagcttg
      781 attgcaagac atacaggtcg agcaaggatg aaaatcggac ttagtgatcc ggtggttctg
      841 cgtggaaggg ccatcgctca acggataaaa gctaccctgg ggataacagg cttatctccc
      901 ccaagagtcc acatcgacgg ggaggtttgg cacctcgatg tcggctcatc acatcctggg
      961 gctgtagtag gtcccaaggg ttgggctgtt cgcccattaa agtggtacgc gagctgggtt
     1021 cagaacgtcg tgagacagtt cggtccctat ccgtcgcagg cgtaggaaat ttgagaagac
     1081 ctgtccttag tacgagagga ccgggatgga cg
//
LOCUS       DAELJO010000412         1094 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.415, whole genome shotgun sequence.
ACCESSION   DAELJO010000412 DAELJO010000000
VERSION     DAELJO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.415"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..901
                     /locus_tag="KPB88_004041"
     CDS             <1..901
                     /locus_tag="KPB88_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG0056219.1"
                     /translation="TLSTIFNKKKKLNIYLKNSILNILSIVSIVYFLFVVLWGINYNR
                     IPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKANETRKLTLQDKNGIVKSN
                     TDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMCYTGITGIYFPFTGEANV
                     NIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHPNIDFNYSGYILALNYTA
                     SALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKINEISNEFNNSYLKANGVT
                     EGTQSYGKMVDLLLTYYELYPYN"
BASE COUNT          412 a          141 c          135 g          406 t
ORIGIN      
        1 tactttatct acaattttta ataagaaaaa aaaattaaac atatatttaa aaaattccat
       61 tttaaatata ttatccattg tatcaatagt ctactttctt tttgtagtgc tttggggaat
      121 aaactataat aggattccac tagaaaccac acttataaat aactataatt taaaaaacaa
      181 tacctctatc caatcaaaac aatatagtgt taaagagctt actaaattat ataaattcct
      241 tgtaattaaa gcaaatgaaa ctagaaaact cacattacaa gataaaaatg gtattgtaaa
      301 atctaataca gattataatg gtgtcataaa tagagctcaa ctaggatacg ataatatctt
      361 ggatatatta cctagtgtta gtggtagtta ttctaagcct aagtatatta tttcatcaaa
      421 tctaatgtgc tatacaggaa taactggtat atactttcct tttacaggtg aggcaaatgt
      481 aaatatagca atacctgact tatatattcc ttgtacagtt gaacatgaaa tggcacatca
      541 aaggggattt gctagtgaag atgaggctaa ttttatagcg tatcttactt ctataaaaca
      601 ccccaatatt gattttaact attctggata tattttagct ttaaattata ctgcttctgc
      661 tttaagtaag gttgattata atgcatatgt tgatatttct gctggcattt cagattctgt
      721 ccgtagagat ttaaaaaatg aaagtgaatt ttggcaaaaa tatgaaggta aaattaatga
      781 aatttctaat gagtttaaca attcttactt aaaagctaat ggcgttactg aaggtactca
      841 gagttatggt aaaatggttg atttattgct aacttattat gaattatatc catataacta
      901 aacactacaa ttcaaaattt tattcatata cagctgatat taaatcagct gttttctatt
      961 taaataaatt atttaacttc aagcaatctt atataatttg atagtactta tactatctgt
     1021 ataacatttc tattctaact attttagtta aaacatttct attatctttt ttaataaatg
     1081 ttttaactaa aatt
//
LOCUS       DAELJO010000413         1094 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.416, whole genome shotgun sequence.
ACCESSION   DAELJO010000413 DAELJO010000000
VERSION     DAELJO010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.416"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            19..846
                     /locus_tag="KPB88_004042"
     CDS             19..846
                     /locus_tag="KPB88_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0056220.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          398 a          113 c          193 g          390 t
ORIGIN      
        1 tagtggggag aaatttgtat ggatttaagc aaaaaagtaa aaattttaga aaatgcatca
       61 gatggcaaaa gcgaatttga gatattagaa tttgaaaaac ttgagggagc ttctagccca
      121 ttaatggcaa tgggattata ttttgctaga gaagctggtt tgaaagctag acaaattaag
      181 ataaaattaa ataattcatc aataaaaaca gaagctggtg cattatacta ttataaagga
      241 aatatatctt ctgaaactaa gattggtggt gttggaggtc tttttaaaaa aggaatatca
      301 ggagcactta ctaatgaatc aatgatgaaa cctgtataca aaggtactgg agaagtttat
      361 ttggaacctt cttttagaca ttatttattt atgagtcttg ataatgattc tataattgtt
      421 gataaaggat tattctattg ttgctctgat acaatagaag taaaagcatt tacacaaaag
      481 aacttctcat ctgctttact tggtggtgaa ggcttcttcc aaatagaatt aagtggtact
      541 ggtgtaatcg ttctggaatg tgttgtacct caaagtgaaa ttgtagagta tgaattgaaa
      601 aatggtgaag aattaaaggt agatggtaac tttgcaatag caagaacatc tggtgttaat
      661 ttttctgtta caaaatcaga taaatcttta attggttctg ctttaaatgg tgaaggtttc
      721 ttaaatacat ttactggtag tggtacagtt tggctagctc caactcaagt aatgtatgaa
      781 aaactaagtt ttggagcaat gcctactaat gataatatga ataataaagt ttctaagcaa
      841 aaatagttgt tttataaata tttattaaat cattattgat tttataaagt cactataaat
      901 tttattagat tattattata aaatcagagt gtggaaatat ttatgtgtat tctttaaata
      961 aaaatttcca cattctgtaa agttattata cttgatatat aaaattcaat ttaggaacat
     1021 agtctaacat ttataaacat attaaggtaa tgatttctct tggtagttat tctcatatct
     1081 tattctttga ttat
//
LOCUS       DAELJO010000414         1077 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.417, whole genome shotgun sequence.
ACCESSION   DAELJO010000414 DAELJO010000000
VERSION     DAELJO010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.417"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            16..>1077
                     /locus_tag="KPB88_004043"
     CDS             16..>1077
                     /locus_tag="KPB88_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0056221.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDK"
BASE COUNT          404 a          110 c          154 g          409 t
ORIGIN      
        1 tttaggagaa aataaatgag aaaattatcc aaaagagaaa aagattttat tcaatatgga
       61 tttttattat ttttgatatg tattactact tatttagtat taactacttt agatataaaa
      121 ctaataccta aaattataag gctagtaaat aagacttata tatttcttgg catattgaca
      181 attatagtat atatgataat tgaatctatt gtaactcatt tgataataaa tgcagttcaa
      241 aaaacaaaag taaaatttat tggtattaaa atggctacaa tgggatttta ctataactta
      301 gttactccat ttgcatctgg gagtcaacca atgcaaatat atgcacttac aaaatatgat
      361 gtaagcctta gtaaatcagt ggctatagtt actaataaaa ctgtagtatt tcaaagtaca
      421 gttactgtat tttgtggaat tttaattttg ctaaataagt cattactgat ggagcaagta
      481 cattctgtga aagtcttgat aacttttgga atgattatga atgtaagtat gttgttaggt
      541 ggactactaa tagtatttag tccaaaacaa gtaaaaatga tagcagaagt tttgattaat
      601 tttttatcta aatttaaaac atttaaattt ttagattcaa aacgtgatag aataaatcat
      661 tatattgatg attataatta ttctataaaa atatttatta aaaatgtaag aatcttagtt
      721 ttaagtatat tacttactgt gttacaatta atcgcatatt tcagtatagt ttattgtgtg
      781 tataaagcct caaatttaag aggagtttca tatgtacata taatgacatt acaggtcttt
      841 ttatatatgg ctatatcacc aattccaaca ccaggaaatg ttggagctaa tgaggttaca
      901 ttttttacta tgttctccaa tataattcca aaagaattat tggggtactc tgtactctta
      961 tatagtgggt ttgtatacta ttttatatta atagtttcag gtttatttac atttggaacg
     1021 cattatacat taaattcttg gaaaaaggaa aataaagtaa attctaattt agataag
//
LOCUS       DAELJO010000415         1062 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.418, whole genome shotgun sequence.
ACCESSION   DAELJO010000415 DAELJO010000000
VERSION     DAELJO010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.418"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..644)
                     /locus_tag="KPB88_004044"
     CDS             complement(<1..644)
                     /locus_tag="KPB88_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0056222.1"
                     /translation="MKLKKLLSLGLAMSMFIANPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGTTNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSIASVAVRDKAPIILTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNS"
     gene            867..>1062
                     /locus_tag="KPB88_004045"
     CDS             867..>1062
                     /locus_tag="KPB88_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBG0056223.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVEKVGGETKKEAEKALR
                     DAINEYENAGIVFDETNMSLS"
BASE COUNT          350 a          159 c          148 g          405 t
ORIGIN      
        1 gaattagtat catttactaa actatcactc attgatgaag tgccacctat tgcataactt
       61 tctattccag tagtattaaa tggcacactt ttcccatcag ttaatattat aggtgctttg
      121 tctctaacag ccacagaggc aatactcata gcatctggtt cacctttgaa agcattagtt
      181 aatattacct tatttacttt attaatagaa tttatttctt ttgctacatt ataacttgtc
      241 ttaatcctat cattaccttg aagccttcta gtctctattc ctttatcttt tagaaaagtt
      301 tctgttgctt tatcaatgga actttctcct cctattatgt ataccttttt agctttttct
      361 actcttttaa gagttgcatt aggtatatta tttttcttag ttaaaagaat tggtgcattt
      421 gttgttcctg caagtccact cgcacttagc ccatcagcca ttgtactatc tgcatttata
      481 agtattgcag tagtataact ttgtttgtca gctatgatac cagcagtttc gtatttatct
      541 gctccttgaa ttttatcaat tttatcaagt gcattagctg atataggatt agcaataaac
      601 attgacatag ctaatcctag cgataataat ttctttaatt tcacaatatc tccccctttt
      661 tcatatcact caattaaatt gtataacaaa aatatattat atgttgtcga aaatacaaac
      721 tatttctact caattaacga cattaaataa cattagaata aattattttc ttataatttt
      781 attctttata atatatataa ttgttataat ataattgaaa cattttactt tgaccacatt
      841 atttaatttt gagaggagaa atttttatga agggtggagt aagaaaaaga ggtaagaaat
      901 ggtattacta ttttgatgca ggcattgtag atggtaagag aaaaaaagta gaaaaagttg
      961 gtggagaaac taaaaaggaa gctgaaaaag ctttaagaga tgcaataaat gaatacgaaa
     1021 atgctggtat agtgtttgat gaaacaaata tgagtttatc ta
//
LOCUS       DAELJO010000416         1057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.419, whole genome shotgun sequence.
ACCESSION   DAELJO010000416 DAELJO010000000
VERSION     DAELJO010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.419"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..285)
                     /locus_tag="KPB88_004046"
     CDS             complement(<1..285)
                     /locus_tag="KPB88_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056224.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQILLNENEKVGG"
     gene            complement(299..>1057)
                     /locus_tag="KPB88_004047"
     CDS             complement(299..>1057)
                     /locus_tag="KPB88_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056225.1"
                     /translation="ALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLNDISTKLV
                     EVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDLMSQLTQA
                     NNTISQLNSKYKYATGTAYARENSSLIACVYDPNTSHTVTETSPYWLDLNGIGFIPDI
                     FFAECEYEPHSNAFYKYFVFAIKNTFPISNNTGFVVNIAFNKGYGDESFKLRGALYTL
                     GKRHVSMDNNGVRVPSLSTLNDFRAYKWHAIKFK"
BASE COUNT          322 a          177 c          131 g          427 t
ORIGIN      
        1 ccctcctact ttttcatttt catttagtaa tatttgattt tctaactctt gtattctctt
       61 ttcttcttcg cttaaataga ttggaatatc ttttaaaata ggttcttttg ttattggatt
      121 tatagattct atatactgtt tactatagtc tatatttcca tattcaacat caatataatg
      181 taattctgtt attgtatcat gctctaatat atctcctgtt gcttctcctg tttggagtaa
      241 tattttacca gtttggtcgt aaattattct atttgctcta ttcattttat cacctcattt
      301 atttaaattt tatcgcatgc catttatatg ctctaaaatc atttagagtg cttagtgaag
      361 gtactctaac tccattatta tccatggaaa catgtctttt gccaagggta tataaagctc
      421 ctcgtaattt aaaagattca tcaccatatc ccttgttaaa agcaatatta actacaaacc
      481 cagtattatt agaaatggga aaagtatttt taattgcaaa aacaaaatac ttataaaaag
      541 cattcgaatg tggttcatat tcacattcag caaaaaatat atcaggaata aatccaattc
      601 catttaaatc aagccaataa gggctcgttt ctgtaacagt atgagaagta ttagggtcat
      661 atacacatgc aattaaggat gaattttctc tagcataagc agttcctgtt gcatatttat
      721 acttagaatt taactgtgat atagtattat tagcttgtgt taactggctc atcaaatcct
      781 gcacactagc gtctgaacta tcaaaacttg tttttatttt ctctgacaac tcaacaagtg
      841 tattatttaa acttgcttct atattcttta atgctaaagt atttataata cttgttttcc
      901 caactttaaa tcctgcatta acctcaacta attttgttga tatatcattt aaatttacat
      961 tttcgggcag tggcattata ttcttactta tacttaacac tttttctgct gtagcattat
     1021 tactgtctgt aacgactatt ttaagtgtgt gtagagc
//
LOCUS       DAELJO010000417         1046 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.420, whole genome shotgun sequence.
ACCESSION   DAELJO010000417 DAELJO010000000
VERSION     DAELJO010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.420"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..575)
                     /locus_tag="KPB88_004048"
     CDS             complement(<1..575)
                     /locus_tag="KPB88_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0056226.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMA"
BASE COUNT          421 a          157 c          132 g          336 t
ORIGIN      
        1 tgagccataa gactaccaga taaagctata agtccatttg aaatcataag tcctagtatt
       61 tttatagaac cgatatttat acctagggat ttaatcattt gagagttatc accaacacct
      121 tttaaagtgt atccaagacc tgtttttagg aataaatcta aaaggacttt acagatgaat
      181 acaaaagcta aggctaaaac tataggagat atttccccat tgaataagtg tttaaagcta
      241 aatagaggaa tatttgattt tcccattatt cttaaattga tagagtatag cattcccata
      301 actaaaatac cagaaagtaa attagatatc ttaaacttaa tgtgaagaat ccctgtaaca
      361 agaccagcag tacaaccaca taagatagcc attaaagttg ctactacagg agaaatacca
      421 ttagtaagag aaaatgctac aatagaagca cccattgtat agctaccatc agcagacata
      481 tctggaaaat ctaagatttt gtaagttata taaaccccca gtgccataat cgataaaatt
      541 aaactttgtg tcattacaga tataatacct gacataaata aaacacccct ttgaaaatat
      601 tttaaatggt ttttaaaatt ttgttttaaa aaatgatttt gatataaaaa aaccacacag
      661 tatatataac tgtatggcaa aaattcgaat aagccacaca gtataatcca tgtggctcta
      721 tattatgatg ataaaataaa aactttaaca acaattacta tagccaacat agatacaatc
      781 cttaaaaatc ataatcctaa ggggcttgta tctatgttat gacaatttgt cttaacttag
      841 gtacaaaccc taactaaatt ggctactaaa aaatcgtact tgtttgttta ataaagtctt
      901 catatttacc tctcctttta atttaataat tttgaaaatt ttgttttctg ttttgtatag
      961 taattagttt aatacagtaa aaaataaaag tcaactataa attttgaaaa attatcaaat
     1021 tatcagaaaa ttgagtttaa ataaaa
//
LOCUS       DAELJO010000418         1040 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.421, whole genome shotgun sequence.
ACCESSION   DAELJO010000418 DAELJO010000000
VERSION     DAELJO010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.421"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(120..899)
                     /locus_tag="KPB88_004049"
     CDS             complement(120..899)
                     /locus_tag="KPB88_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG0056227.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVRGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT          411 a          172 c           92 g          365 t
ORIGIN      
        1 ttatttttta ataataaaaa ctgtcttttt aaaaaattct tataaaagac agttttatca
       61 atgttatact ttaatgttta attaaagcca tattttattt aaatatacaa ctatctatct
      121 tagtacatag tataagattt ttctttatct aatagtatat cctctacatc tgggttttct
      181 tttactaatt gtctataaaa attacaatta gtaacaccca catatggaat taaccatagt
      241 agacctattc caagagtagc tattgaaagt atataccatc ctataaaact aaagtttagt
      301 acaaataatt taagtacatt tcctttcatc atatctaaac tagcacccat tgatttaaat
      361 acacccatat tatccttgtc ttcacaaatt aagaaagtag cttgagaaac atataatagc
      421 aatatataaa ctggtaacat aagaacaagt attataagaa ctagagttat tccagtagaa
      481 ataatagtcc ctattccaac attatcactt gataaatatt caattgctcc actagtaagt
      541 aaaactccaa atgataaaaa cgctcctata attgtgatta taaaagaata aatataaaca
      601 ataattgatt caaataaata aaaaccaaaa cttcttaaat aaattctccc tcttacaaag
      661 atatctccta tcttgacttt ttcatcttta gtaattttca agaacataca tgtaaatcct
      721 gctgaaacgc aaaaagctat tacagccact acaatagtag tcaatggttc aaaaggtaat
      781 cctacaattg atattaatac tatcaatgca atatatactg ccatagaaat tgcacatatc
      841 ccccagtttc cagataactg agctttagat actcgtttta attcttgcct tgataccata
      901 aaaacacccc tttcatttta taaaaaaact tttaaaaata taaataatct tttaacgtat
      961 aatattaata tatcatattt tcgactatat aagatacaat tattcaccat gttttatcaa
     1021 gatatgccat aattgtatta
//
LOCUS       DAELJO010000419          995 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.422, whole genome shotgun sequence.
ACCESSION   DAELJO010000419 DAELJO010000000
VERSION     DAELJO010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.422"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(228..713)
                     /locus_tag="KPB88_004050"
     CDS             complement(228..713)
                     /locus_tag="KPB88_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0056228.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(796..889)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          337 a          139 c           78 g          441 t
ORIGIN      
        1 tttttttaag atttttcaaa gtagtattaa tttatgatac ttggtgtata aagtataagt
       61 taaatctact ttcaattaaa tttaccattt ataattactt tataataaaa ataaattagt
      121 taatttattc tctttagttc tgttataatg tttctacaaa aaagaatccc attaatggga
      181 ttcttttttg tgatttatta atatatttaa agcttgctag tatcgtatta taaacttaaa
      241 tgctcttcta tgtattgatt atatacatat ccagaatatc gtttttcatt tttattgata
      301 ttaactcttg cccattttcc aacgctataa catacttcaa ctttaaaacc atcagggatt
      361 gtagttataa ctttagattc taatgttcta tcatttctaa gttctaaact taatccattt
      421 gttcttatat ttcctatgta attctttttt ctatttttta ttgtatcatt actattgttt
      481 ttagcattac ttaatctttc taaatattta taaagtaacc aacctttgtc ataattactc
      541 tcaacttctg cccatattcc atctacacac tttaacttaa caattttacc ttctggtata
      601 ctttttaatt tcaaactatt tatgtctggt ttttctctta aattcaattt aattttagtc
      661 ttaaaattga tgctagattt gcgaaaattt tttaacatta attcagatat catcttaagc
      721 cctcctcttg ccaaatatta agttattaaa atatataact ctaaaaatac ttttaacaaa
      781 aagaattatt atctttttgg caactggctg acatgacctt tattacataa agattttagt
      841 ccctatagtt ttgcgtcatt agatttatct aatattgcct atatctgaat aataacataa
      901 tttgaacaat taaaaaataa tattacctca aattttatat tatttaaata taaattaaat
      961 ttttaattaa taataatcaa aaaatatctt tatat
//
LOCUS       DAELJO010000420          979 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.423, whole genome shotgun sequence.
ACCESSION   DAELJO010000420 DAELJO010000000
VERSION     DAELJO010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.423"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            45..797
                     /gene="cwlD"
                     /locus_tag="KPB88_004051"
     CDS             45..797
                     /gene="cwlD"
                     /locus_tag="KPB88_004051"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG0056229.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          430 a          103 c          153 g          293 t
ORIGIN      
        1 aagatatatc aatattaaaa aaatttaaat gtttgtacta atatatggaa tattatagtg
       61 tttttataag tatacatata cagggtgatg ttgtgagaaa gtacataaaa catataattt
      121 ttagttttgc aatgatatgt ctagtagtag tatccatatt tgaaattaaa aatatttctg
      181 aagatgttat caagtatatg ccagtaacaa acaaaactat aattttagat gctggtcatg
      241 gaggcattga tccaggtgca ttaaataagg ataagagtac atctgaaaag gatattaacc
      301 tagcaataac acttaagctt agagagctta tagaatcaag tggtggcctt gtaatattaa
      361 ctcgagaaga tgacagtagc ctttataaag aagaaaataa taaaactaca agacaaaaat
      421 ataatgagaa tttgaaaaat agaaaagaaa taatatctaa ttctaatgcc aatatgtttg
      481 tctctataca tcttaatgca tttgaacaat caaaatacta tggagctcaa actttttatc
      541 ctaaagacaa acaagacagt aaagaattat cgaagtgtat tcaagaagaa cttaaaagag
      601 tagtagataa gacaaacaac agagaagtca aaccaaggga tgatatatat cttttgaaag
      661 aaaataatat tccatcagta ctgatagaat gtggtttttt atcaaatgaa aaagagtgta
      721 aactcttaac tgatgaaaca tatcaagaaa aaatagcatg ggcaatctac ataggaatac
      781 aaaaatattt aagttgaaat aactataaaa ataggtctaa atatgtaaat agcttgtatt
      841 aaagcagtta aaatgcatta atataggcta tttttatttt gacaaaaaaa tatttaaaat
      901 aaaagttaaa aagttgttga cttagaataa tatagatgat attatatatg agtgcccaaa
      961 aggagcacca aaataagac
//
LOCUS       DAELJO010000421          969 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.424, whole genome shotgun sequence.
ACCESSION   DAELJO010000421 DAELJO010000000
VERSION     DAELJO010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.424"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(86..274)
                     /locus_tag="KPB88_004052"
     CDS             complement(86..274)
                     /locus_tag="KPB88_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056230.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            527..676
                     /locus_tag="KPB88_004053"
     CDS             527..676
                     /locus_tag="KPB88_004053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056231.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
BASE COUNT          364 a          112 c          106 g          387 t
ORIGIN      
        1 aaaaaagtaa atatgtaacc ccaacacatc tttactaagt caaacattat tatatagagc
       61 attcttcatt atggagagtg ctttttcatt tctttgaata atcatgttga ttatttataa
      121 tattttcatt taatttatct ttatctatca aatttaatgc acaattaata caaatgttag
      181 ttttcatatg acttttctta tgaaaagtaa tatacttatt atcatcttta tttattcctt
      241 tattacagta atcacataat atagtcttag tcatattttt attcctttca ttttatattt
      301 tccataatta agtttaacat atttagtata tacctatgta tatatattta aaacaatgtt
      361 ttttattgca atatagaata tattgtataa ataaaaaaat atggagaatg gatatttgat
      421 aattgtaaaa ttatgttata atttatatgt aaggaactaa atatctaaga gtggcagttt
      481 ccacattatg ctctaaccta ctttctaatg aaaggaggtg ggaagtgtgg aaatagtagc
      541 agtttattac atagcaaaat ttttacttga tttatacaaa gtaaaaagcc actcaaagtc
      601 ggaagctaag agtggcttta tatttaattt aactattaat aaactaatag taaaaattaa
      661 atataaaaag tactaaattt tttcggaaac tacactctcg acaatagatt atagttcctt
      721 atttctaaag aggtaggaga attgcagttc tcttatctct ttttgatacg aatttacatc
      781 tttattatat cgcattttga gaaaaaataa aactatgaat attagaaata tattttattt
      841 tatctctaag tatatatatt attagccacc taaaaaactt tcacaaaaac aaaaagagta
      901 tatgtttttg ataactggat gatataactt ataactcatt ttacaattct tagttcatat
      961 atatttatg
//
LOCUS       DAELJO010000422          958 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.425, whole genome shotgun sequence.
ACCESSION   DAELJO010000422 DAELJO010000000
VERSION     DAELJO010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.425"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            168..356
                     /locus_tag="KPB88_004054"
     CDS             168..356
                     /locus_tag="KPB88_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056232.1"
                     /translation="MKIIYKNKVYKVEQDKVLFRITYYDEQRGSKKFNKDKKVKRSTL
                     TRDIEVVNLYLPTHLKIK"
     gene            388..528
                     /locus_tag="KPB88_004055"
     CDS             388..528
                     /locus_tag="KPB88_004055"
                     /inference="COORDINATES: protein motif:HMM:NF015807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0056233.1"
                     /translation="MQREQTTIRLPKELKEKLEKQASKKGRSFNSILLSILQEFIQNP
                     NV"
     gene            complement(506..694)
                     /locus_tag="KPB88_004056"
     CDS             complement(506..694)
                     /locus_tag="KPB88_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG0056234.1"
                     /translation="MAKMGTYKNPHFAIRIPKEKLDKLKYIAEYNARSANKEIEFLVT
                     KHIEMFEKEHGSIHLGSE"
BASE COUNT          420 a           99 c          158 g          281 t
ORIGIN      
        1 aatcaaggaa aaaacatcaa agaaatgctt agagaaatga aagaacttag agagggggtg
       61 atttagttga gtgtaaaggt gttaatagct tatgtacagt tttgtaagca atataataag
      121 aaagcaagtt ttgaaagtct taaaaaatac aacaaagggg taattgcatg aaaataattt
      181 ataaaaacaa agtttataag gtagaacaag acaaagtgtt atttagaatt acatactatg
      241 atgagcagag aggtagtaag aagtttaata aagataagaa agttaaaaga agtacattaa
      301 caagagatat agaggtagtt aacttgtatt taccaacaca tttaaaaata aagtaagcta
      361 taaataatta aagaaaaagg tgattagatg caaagagagc agacaacaat acgcctgcct
      421 aaagaactta aagaaaaatt agaaaagcag gcgagtaaaa aaggaagaag ttttaatagt
      481 attttattaa gcatattaca agaatttatt cagaacccaa atgtatagaa ccatgttctt
      541 tttcaaacat ttctatatgt ttagtaacaa gaaattctat ttctttatta gctgaacgag
      601 cattgtactc agctatatat ttaagtttat caagtttttc ttttggtatt ctaattgcaa
      661 aatgaggatt tttatatgta cccattttag ccataatatc aactcctttg tgtagtctat
      721 ttggcaacaa ttatatcaca aaaataaata caaaaaatat ataggcaaca tatagacaac
      781 ataataataa agtgttatac tttgtattaa agataggcaa cacatagata acagtacagg
      841 aggaaattat gttggcaaat agagtaagaa ctggattaag aatcccatat gatttaaata
      901 caaaattaat attaatagca gaagaaagag gaatgtcaaa aaattcttta atattaca
//
LOCUS       DAELJO010000423          951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.426, whole genome shotgun sequence.
ACCESSION   DAELJO010000423 DAELJO010000000
VERSION     DAELJO010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.426"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            162..821
                     /gene="cprR"
                     /locus_tag="KPB88_004057"
     CDS             162..821
                     /gene="cprR"
                     /locus_tag="KPB88_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG0056235.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          384 a           56 c          178 g          333 t
ORIGIN      
        1 tttgaaatat aaagtttttg tatctaaaat attttatttg taaaatagta attaattatt
       61 gtaagctata atatgagagt attatggtta atgtgaattt taaagatatt attaaatata
      121 taaattagaa tgctattaga tggataaatg gaggagttat tatgtctaag atattagtag
      181 tagatgatga gttagatatg ttaaagttga ttgaaaatgt gttgaaaaga gatggacatg
      241 aagtaaaggt aattagtgat gtagaaaagg ctatgaacat ggattttaat tattttaatc
      301 taataatatt agatgttatg atgcctaaga tggatggttt tgaagtatgt aaaactataa
      361 gaaataaggt tgattgtcca atactgtttt tgacagcaaa aaatatggaa agtgatatta
      421 tgtacggtct tggtataggt gcagatgact atataaccaa accttttggt gtaggagaat
      481 taagggctag gataactgct catttaaggc gagaaagtag agaaaaaaag aatttattaa
      541 ctatctcaaa tgttcaattt aactttttag gcaaagaagt ttcaattgat ggtaaaaaaa
      601 ttaattttac taaaagtgaa tatctaatat gtgaatttct agcaaaaaat aagggacaag
      661 tattttcaaa agaaagaata tatgagtcgg tatatggttt tgatggagaa agtgatataa
      721 gtactatcac agagcatata aaaaatatac gaagcaaatt aaaacaatat gatatttata
      781 caatagaaac tgtatggggg atagggtata aatgggtgta ataagtataa gtgttgttta
      841 gtgaaaatat tatatatatc tacaaaatgt ttgtaaaata ttaatctata tattaaaatt
      901 taatttatag attaatattt tttgtaaatg acaaaattgt aaggtatttt g
//
LOCUS       DAELJO010000424          949 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.427, whole genome shotgun sequence.
ACCESSION   DAELJO010000424 DAELJO010000000
VERSION     DAELJO010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.427"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            226..915
                     /locus_tag="KPB88_004058"
     CDS             226..915
                     /locus_tag="KPB88_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056236.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
BASE COUNT          419 a           61 c          190 g          279 t
ORIGIN      
        1 aaatatagct attatagcag agcgaggaag agttagaata ttagatttag aaactaaaga
       61 aaaatatgat ttattaaaaa ataaactcga ggaaatgtta gaagaaatat aagtgaaaat
      121 atctaattaa gacagtttag agagttgcaa aatgtctttt ggtataaatt attgttgaag
      181 tgttttgtga ctctcaaaaa tgaaaataga gaggggaata atataatgga tttaaataaa
      241 attatgaatg atgcactagt agagttagag gaaaatggct ttgtagagga agtagttaaa
      301 aaacaattgg cagaaacaat aaaaagagtt gtgaatgagg tttttggaaa ttggggtgat
      361 tttagtaaaa aaattgaaaa acatctaagt gaaaatataa atataaattt agataaatta
      421 gatatacaaa aatataatgt gctcgtagca actacagtaa aagaaaagat tgatatgaca
      481 atgaaggtag aagctattga acacttaaaa agaaatttag ataacatgct tgtaggtgct
      541 gaaaaagaat ataaaatgag cgaattgttg gaaatattaa aaatggataa atatgatatt
      601 gatgaatatg aaggctatga agattgcata acatttattc ttgatgaaaa taaatatggt
      661 tcgggatgga ttgaaattga taaagaacca aataaaagta aatacagatg cgaacactcc
      721 atattgttga gaaaagatgg aactattgca tcatatagat acgagaatag agaaatatca
      781 tctaaagata taatgagtgg atttgataat tttggagatt tgctatttaa aatatttgct
      841 catgggtcaa aaataatttt ggatttagga aatgatatag aagattatga tttaacaaat
      901 ggagaagatt attaaaatag gggatggaac aaatgtcaaa gtatgtatt
//
LOCUS       DAELJO010000425          938 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.428, whole genome shotgun sequence.
ACCESSION   DAELJO010000425 DAELJO010000000
VERSION     DAELJO010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.428"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..667)
                     /locus_tag="KPB88_004059"
     CDS             complement(<1..667)
                     /locus_tag="KPB88_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0056237.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLD"
     gene            complement(782..>938)
                     /locus_tag="KPB88_004060"
     CDS             complement(782..>938)
                     /locus_tag="KPB88_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0056238.1"
                     /translation="KMDIVKYEEFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASK
                     LLIGLYE"
BASE COUNT          323 a          151 c           74 g          390 t
ORIGIN      
        1 ggtccaactt actatttatt ttgtttattt tttctctaac ttctaattcg tcgttattta
       61 ttgttttaaa taaagcttgt atttctcctc tatcaaattt atccaatttc acatttatat
      121 cattaaggtc tgtaattata tcgtctaata agtttaactt tatatctaat tttttaagtg
      181 ttataatagt aacaatcaaa tcaagtatag tccaaagtgt tgtaattata gccattgaaa
      241 ttaatattct atatggaatt atacttacaa aatattttat aaatggatgt ataaatctta
      301 ttattaaaac agacattaca ccaaagtata ttgcattttt taaacatact ctacccttta
      361 tattgaattt tctgtcagaa taatcccacc atttagcatg aaataaggtt tctaaaataa
      421 atcctgtaaa atattctagt atcgtagtta aaatcattcc taatagaaat actattacta
      481 cattattaaa tcttcccaat agtgatatca caattaccgc tccaaaacca taaacagggc
      541 atattggacc atttaaaaaa cctctgttga ttattctttt gcttgctata gaacatccta
      601 tactttctac tgtccatcct aaaaatgaat atataaaaaa gtacaagata tattttatta
      661 tctccataat ttatctctac tccttcacta atgttcttat tttatgcaca acgtaaaaat
      721 aattatagaa atagtttttt cgttttttat atttgttatt cttttattct tgtttaatat
      781 tttattcata caatccaatt aataatttac tagccataat accatgttta tcctttggct
      841 gattttcttt atcaatccaa acattaatag tatcatatct ccttaataaa tctaaattct
      901 tcacatcaaa ttcctcatat ttaactatat ccattttt
//
LOCUS       DAELJO010000426          936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.429, whole genome shotgun sequence.
ACCESSION   DAELJO010000426 DAELJO010000000
VERSION     DAELJO010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.429"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            117..257
                     /locus_tag="KPB88_004061"
     CDS             117..257
                     /locus_tag="KPB88_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056239.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(254..625)
                     /locus_tag="KPB88_004062"
     CDS             complement(254..625)
                     /locus_tag="KPB88_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056240.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          352 a          102 c          117 g          365 t
ORIGIN      
        1 tggataatca aaatatattt tataaaatca aaatacaatt acatgaactt tattaaaaat
       61 ttgttgggaa tttctgacta acattaagaa cgtaagtttg ttgggggatg gtatatgtgg
      121 aaaagtcaaa cactactgtt acaaaaaaag aaaaaattga agatatagga atgaaattag
      181 aacatttatt gagtgaagat attacttttt atgaattatt acttgagatt ttgtataaag
      241 aaaaagaaga gaattaattc tcttctttaa ggaatctttt taaataggca ttatcaatca
      301 ttttttctat agcttctcta tcttcttttt caagagaata tattttttct aagagctttt
      361 ttgttttttc acttactgat aagttctcta tgattaattt atcttctttg cttatttttg
      421 tattatcatt tacgcctaat aaataatctg ttgatgtttc taatgctttt gctaatctaa
      481 ctattatttc acttttaggt tctcttaaat tattttcata acgtgacaag ctagcctcag
      541 ttataccgac tttagtagca agttcttttt gatttatatc taattctttt cgtagatttg
      601 ctattctatc tcctaaactt tccatatgta catcactcct taccgaaatt ataagttatt
      661 ttgataaaaa gtaaaataaa attaccaaaa atataaaaaa aaagggttaa aataataaaa
      721 tatattgaaa attaccattt gtatatgtat aatatgaatt atacaaatgg taatttgatg
      781 agaggtggta tgttgaaata agaaactgaa ttaattaata aatatataga aagttttgaa
      841 aacccttaat tgagatataa acaatatgta caaaattttt tgcaagtaaa attaccaaat
      901 gtataaaata aatcagtagc atagataatt ttatgc
//
LOCUS       DAELJO010000427          934 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.430, whole genome shotgun sequence.
ACCESSION   DAELJO010000427 DAELJO010000000
VERSION     DAELJO010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.430"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            206..346
                     /locus_tag="KPB88_004063"
     CDS             206..346
                     /locus_tag="KPB88_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056241.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(520..840)
                     /locus_tag="KPB88_004064"
     CDS             complement(520..840)
                     /locus_tag="KPB88_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0056242.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
BASE COUNT          365 a          106 c          114 g          349 t
ORIGIN      
        1 tttaatatat aaaaaagata ataatagtta gattttttat atgaatttta ataggtaaaa
       61 tatcctattg ttttgtagat atatcctaat tatgtcaaaa aataaaaaaa tttttattta
      121 ttagagtttt tggaaataat aataacagat attataatta attaacagtt tttgtaagta
      181 tttgacaata gagagggtgt aacaaatggg aataatagag tcagcatcaa aattggctga
      241 gatggttcat ttattggctg ttgaaaaggg gataactgat atagaagcat gggatgaagc
      301 tgtgaaagaa tacagcaaaa tttatgaaga aagaagaaat gaatagaatg ataaaaaatg
      361 aaaacttgat gataaaaata actttattta aagaaattca atttttatag aagtattaaa
      421 aataatataa ataaaaagat ggtatttcaa atgtaagtaa aaaatcattt tgggatacca
      481 tccttattat ttattttaat aatatataag taataaatct tacttatcca tattttttat
      541 gtaacctctt ttttctagct tttttatatg ctccttacgt atttcatcaa gattaatacc
      601 atattgttct tctagcttaa acatcatagt aacacatgtc tgcgctacat ccattagttc
      661 cttagcagtt ttctttataa tatcaacttc atctaaatca ttattttctc cacttagatt
      721 tctgaattta cctatacatt cagctaattc accttgttct tcacccattt tgataaatgt
      781 agattctagt gttgggtcta aattattaag ctctggtaaa gatatagttt ttagtttcat
      841 aaaagctcct ttcaagttat tcgtatttat actaaaacat cataacaaaa ataatactaa
      901 aaaaaaactt taaattcaac ataaaaactt acat
//
LOCUS       DAELJO010000428          932 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.431, whole genome shotgun sequence.
ACCESSION   DAELJO010000428 DAELJO010000000
VERSION     DAELJO010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.431"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            218..868
                     /locus_tag="KPB88_004065"
     CDS             218..868
                     /locus_tag="KPB88_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0056243.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          399 a           92 c          162 g          279 t
ORIGIN      
        1 tttgaagtac aatggtttaa tctatagtac aataacttga tttgcaatac aatgaattaa
       61 tctatagtat aagagcatga tttacagtac aataatttaa attgtagtac aaaagtacta
      121 tcaaataaga atttctactt taaaaatatt actaaaagta attaaagtcc agtaattcaa
      181 tataatatgg caaaagaata gaaataaggt gagagtagtg aaagtaaagt caaatttaga
      241 tgaaatttta tatcataaga ttattgaaag tcttattcgt ggtgaatact ctgtaggtca
      301 aaaaatatta ctaaatgacc tttgtgaaaa atttgaagta agcagaacac cagttgtaca
      361 agcagtaaaa atgctaaata aagatggagt gcttactatt atgacaaatg gtaaagtgta
      421 tgtaccagag tatgaatatg atatggtaaa acaagtttgt gaaacgagaa cactgattga
      481 aacatatgct ttagaaaaaa tgatgcaaga agaggaagag gtttttcaac aaaagctaga
      541 tgtaataaaa agatactctg ataaatgcga ggagtactat cgtcaagaaa aatctgtaga
      601 acttgcacta gcagatttgg aattacataa agccattgta gaaggggcaa ataatgagat
      661 tttaaatgat gtatatgttg gtatacaagg aagatttata gtagtgaact atttaattcg
      721 accattaaaa aatcgtaatt atgaaggaac agtacaagac cattttgaaa tattaaaatt
      781 cattgaagac agagatacaa aaaaagcagt agataaacta agaaatcata ttcaaggaac
      841 tattaaaaga ttctgtgaag atgaataata agttttgaag ttactatata tacttatcta
      901 ttaagttgct aaatgtagtc taagtataat ct
//
LOCUS       DAELJO010000429          914 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.432, whole genome shotgun sequence.
ACCESSION   DAELJO010000429 DAELJO010000000
VERSION     DAELJO010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.432"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(66..851)
                     /locus_tag="KPB88_004066"
     CDS             complement(66..851)
                     /locus_tag="KPB88_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0056244.1"
                     /translation="MNFENDFITPEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          320 a          146 c           93 g          355 t
ORIGIN      
        1 taaaaaaata aacagttagt aaaataaatt tttactaact gtttattttt attgtttaat
       61 atgtcctata aatcctctaa gtaatatttt ccttgcaaaa attctgattc taaacctaga
      121 tttttaaaat aatcgaccat gcccatacac gcaaattttt ctgtagaata atgagtagca
      181 cctataacat taattaaatt atcttttgct atttgatgga actctaatac tggttcaaaa
      241 tgttttaaag gcttagtaaa cccagtaata tatagattta taccaagttc agcaacttct
      301 cttgctacaa atggataact tcctccccct gctgcaatag caactaaacc atcttttaat
      361 acagaatctc cataatcata caatttcaca tcatgaccaa cagatttttt aattaattcc
      421 ttaatctctt ctattgattt tagtgatgtt ttacatataa ctcctacttc aattttatcg
      481 tcatatttac aaaatggttt gactatttct aaacccatag cttttgcaaa actaacagaa
      541 gttgaatagt cactatagtt gtctagtgga gaatgtaata cataaaaaga aattctacga
      601 tttttcatct cttccatata agatagtggt atgtcgtgaa atggaaaacc ttcatctgat
      661 gctatgtatc ccattgcatg atgtgaaaaa attaaaatat ctttttcatc tttatctata
      721 acttgcttaa ttatttcttt atcaggaaaa gtagttgtgt atactttatt aatattttga
      781 gcattatcta atactaatcc tatgtatttt tttctaaatt ctggagtaat aaaatcattt
      841 tcaaaattca taaaactcca atcatcatta atatttttaa tattaaattc taaatctaat
      901 tttttataaa gttc
//
LOCUS       DAELJO010000430          906 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.433, whole genome shotgun sequence.
ACCESSION   DAELJO010000430 DAELJO010000000
VERSION     DAELJO010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.433"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            379..>906
                     /gene="yidC"
                     /locus_tag="KPB88_004067"
     CDS             379..>906
                     /gene="yidC"
                     /locus_tag="KPB88_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0056245.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAKKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLI"
BASE COUNT          357 a           75 c          140 g          334 t
ORIGIN      
        1 ctataattat ttaagactaa atataaatgt tggaattatt taggacttga aattgtctaa
       61 aaataaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata gtatgttata
      121 tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt atttttgtaa
      181 agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg taaatagaat
      241 tacatattct gaagaacggt tgcctaaaca gtatatagtt ttccagaaat atgatatttg
      301 gaggtattgt agtttgtttt aatttgaaat gaatctatta taaattctaa agtttaaaaa
      361 taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt attaagtata
      421 ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt aatagttaaa
      481 ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca gcaagaatta
      541 gctaaaaaaa tggaatatat aaaagaaaag tataagaatg atactaaaga attggaaaag
      601 caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgtct tatagttttg
      661 gttcaaatgc cagtagtata tgctttatat catgtatgtt tagatatgac aaaggaattt
      721 acaacaatta taattccatg gattgctaat ttaggtacat ttgataattt gtttatatta
      781 ccttgtatat atacattaat tatgttagct tcaaatttag tgaattatat accatattta
      841 aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac cataacaagt
      901 cttata
//
LOCUS       DAELJO010000431          889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.434, whole genome shotgun sequence.
ACCESSION   DAELJO010000431 DAELJO010000000
VERSION     DAELJO010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.434"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(163..720)
                     /locus_tag="KPB88_004068"
     CDS             complement(163..720)
                     /locus_tag="KPB88_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056246.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT          296 a          127 c           82 g          384 t
ORIGIN      
        1 tttagttcat gagacactct tttttgtata aaatcaaatg cattttcagt attttaacaa
       61 taaaaacagg attatctata ttttgaggca accactttat tgaaatttta tttagaaaac
      121 aatcattact aaataaccag catagatatt aaatgattat atctattgtt ttttcaattc
      181 atattgataa caaggaacat tttttacagt atttccagta tgaatttttt tgatatactt
      241 tcctgtacat ttaaatccta ataaatctaa tgattttgta gaattttggt tttcttcata
      301 ggttattgca attattttat ttaagtttag ttctctaaat ccatactgta ttactctttt
      361 tgttatctca gtagcaaaat catgtcccca atagctcgga tttatacata tacctatttc
      421 tgcttctaat gctttaggat ttatattatt aaaccccata tttccaataa ctttattgtc
      481 tcttttatat actatagcaa agtggccaat tttccctttt ttatatggat ttagaaaaaa
      541 tgattttata aactttcttg tatcttctaa agatttatgc tcactaaatg gtaaatgttt
      601 tactactatt ttatttttat aacagtcaaa aaaatcttca acatcatcta atgatgcagg
      661 tcttagtata caacttctag tttctaaggt tttcatttga ggatattcag aatcagacat
      721 ttctaatccc cctttataca ttaatctaaa tatactgttc atatttaaaa tacgatatta
      781 attatataaa tgttctcaaa ttaaccttat ttttattttt aataaaaact aattactata
      841 tgaatctttc aagtttacga atttatctct atttttatat taaattact
//
LOCUS       DAELJO010000432          886 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.435, whole genome shotgun sequence.
ACCESSION   DAELJO010000432 DAELJO010000000
VERSION     DAELJO010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.435"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(4..714)
                     /locus_tag="KPB88_004069"
     CDS             complement(4..714)
                     /locus_tag="KPB88_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0056247.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          283 a          125 c          105 g          373 t
ORIGIN      
        1 aaattaagat tctaacatat accttgtaaa ttttcctaca acctcttgat attttttacg
       61 gcttatagga attttttcat tctgaacttt ggcataagtt tgagttatac tatctacatg
      121 gtttagattg acaatatacc tttgatgcgt acgtataaaa ttagagggaa gaacagtttc
      181 catatcattt aattttccgt agtattctaa tgttttatca aaggtaacta cagttaattt
      241 tcttagctca cttttaaagt agaggatatc ttttgtatta attttacaaa gattagttcc
      301 aatctgaact gtaataaagt tttctttcca ttgttctaat ttttttagtg cttcttttaa
      361 tactttacat attttttcag ttttataagg ttttaagatg tagttcaaag ctcctacatc
      421 atatccatga aatacatagt cagaaaaacc tgtgacaaag atgattatag tatcttttga
      481 atgttttcgt atagtttttg ctatgtcaac gccattactt tttccaagtt caatatctaa
      541 aaaaataata tctatgtaat aattttcgtg ttctagattg tatactagac tttctccatt
      601 tgaatattct tttatttggt aggggtagcc ccacaaatcc atttctcttg taatgatttt
      661 attcagcatg tttctttgtt gtttttcatc atcacaaata caaatcgaag tcaatttttt
      721 atctcctttc aagttttata tttatatagt taagtatata atatattttg ctacctagat
      781 atataaaatg aaataacaat tatatccaga tagcaaaata aaatttttta tattagtata
      841 cttacactta aaactattct attaaactta ttctgaagtg ctaatg
//
LOCUS       DAELJO010000433          876 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.436, whole genome shotgun sequence.
ACCESSION   DAELJO010000433 DAELJO010000000
VERSION     DAELJO010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.436"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..775
                     /locus_tag="KPB88_004070"
     CDS             <1..775
                     /locus_tag="KPB88_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0056248.1"
                     /translation="SYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCV
                     LLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDC
                     ELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNE
                     KYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGMEEKDVTNSAIVTMIEFTYAE
                     APKSMRERYKINCEFLGLIPKDSDKIGVGVEYYDSLLND"
BASE COUNT          355 a           78 c          134 g          309 t
ORIGIN      
        1 atcttatgat gttgatgata gagagattgt aaatctttat aaaaatatat tagaagaatt
       61 taatataaca aaagatataa aactcaaata ctcttatgaa gttgaaacac cagcattttt
      121 taattcatgt gtacttttgc caccacatga gtataaattg aaagagttag actggatttt
      181 tagacatgag cttatgcatt ttaagagtag ggacctttat ttaaaatatg taatcctgtt
      241 tttaaaaaca gtatattggt ttaatccatt tatatatata atggataaac atatagattt
      301 agattgtgaa ctttattgtg atgaaagagt tttaaaaaat cgaaattcag aggaaaaaaa
      361 ggattatgct ttaacaattc taaatgcaat gagaaaaggt tcaaatactt caaataaatt
      421 tatagctgga cttcataagc aatctgatat taaaaagaga gttatgaaca tgtttaatga
      481 aaagtacaag aatggtatat taatggcatt aactttatgt ttattatctt ctattacttt
      541 tttgaaggtt aattcaacta gtattgttaa tcttaatgta ttgccaaaag gaatggaaga
      601 aaaagatgtt actaatagtg caattgttac aatgatagaa tttacatatg cagaagctcc
      661 taaaagtatg agagaaagat ataaaataaa ttgtgaattc ttaggtttaa tacctaaaga
      721 ctctgataaa ataggtgttg gtgtagaata ttatgacagt ctattgaatg attaaataaa
      781 tattatttta acagatatta attataaagt tttgaatagt aatttgttga gaaataaata
      841 tatccttata aaaatagata cttatatacc aagaat
//
LOCUS       DAELJO010000434          862 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.437, whole genome shotgun sequence.
ACCESSION   DAELJO010000434 DAELJO010000000
VERSION     DAELJO010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.437"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            258..749
                     /locus_tag="KPB88_004071"
     CDS             258..749
                     /locus_tag="KPB88_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG0056249.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKNFIQEASNDGLAVIF
                     FIM"
BASE COUNT          339 a           78 c          135 g          310 t
ORIGIN      
        1 tgattctaca gtataatttc ttaaattttt ttaaaaagta atagttctta tttatagtca
       61 atattgccta tagaacttct gattcattta atttttagga aaaaataata atacttacgc
      121 tataaacaca gaattttagt tgataatata ggtatttaaa tttaagatga aataatactt
      181 ttctcaatat attttattgt aactaagttt aattataagt tacaattaaa aaaacgctac
      241 ttaggaggaa tgtaattatg gggatgatag gatgttatac taaaataagt gaagagaatg
      301 tttttaaatt acagcaagca gaagaaaatt tacaagattt tgtttttgaa gatgccaacg
      361 aaaatagtac tatcaatatt gataaagcat ggcatgccat tcactttact ttgacaggtt
      421 gtccttttgg tggagatgat gataatatat ttagcaaatt agtactaagt ggaaacatac
      481 ttatggaaat tgatggagaa tttcctgtca tgttgattac agcaaatgat gtaaaaaaac
      541 tatcaaaagc aatgaacagt ttggaagaac aagcatttag aaaaagattt aatattaatg
      601 agatgttaga aaataatatt tatcctgtta tgaatgatga gagtgaagat gatttttttg
      661 aatatgtatg ggcaaatcta attgagctta aaaattttat acaagaagca tctaatgatg
      721 gactagctgt aatatttttt ataatgtgat aattactaaa aattgtgaat attagtgtca
      781 tcacatatct tagatatgaa gccttttgat tgtatagaag aaaatacata ttatattagc
      841 cttttaaaat atataagtta aa
//
LOCUS       DAELJO010000435          831 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.438, whole genome shotgun sequence.
ACCESSION   DAELJO010000435 DAELJO010000000
VERSION     DAELJO010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.438"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(300..677)
                     /locus_tag="KPB88_004072"
     CDS             complement(300..677)
                     /locus_tag="KPB88_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0056250.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          319 a          125 c           60 g          327 t
ORIGIN      
        1 tcaaaacaaa aataaaatta tgccctttat atctaaaaca attaatttac atgattaata
       61 tacaaaaatt ctgactttta aattcgtata caatatcaaa ctaaaataat attaatgaca
      121 aacccaatat aaattaaatt caaacaggtt tgattttaca tcaaacttat tgtattaata
      181 ttaaataaca agacaaaaat atttataaaa atcacaaatt ttacataaaa ctttatattt
      241 gattcattta actaattatt gtaaaaacta tttattttat gataattaac tacaccaaat
      301 tattcatctt ttttaatatc cttaacccat ttttcaacag agttcaattc ttcttcattt
      361 accccctttt tgaacaagga tttcataaaa ctctcaatag aacttccatg catattatct
      421 aaaaaagttt ttgtttcaaa atgtaaatac tcttcttctc caataagaac tgtataatgt
      481 gtatgtctat caatttttct tgcacttaag aattgcttat ttactaatct tgataaaagt
      541 gttagtgttg tagtttgctt ccaattatat agtttttcca tagcttcaat gacatctttt
      601 gatgctactg taaaatctga actccaaata aatttcatga cttttaattc tgctggtggt
      661 atcttagtaa tagtcatatg attttacact cctctcaaac ttctacataa ttaaatacta
      721 tattaataga cttttcgagt caaaggtttt atttaaaata tctcatccaa atttattaat
      781 acaaaaaatt tatattttta ttcaaaatta aacacacaat ctattaatat a
//
LOCUS       DAELJO010000436          819 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.439, whole genome shotgun sequence.
ACCESSION   DAELJO010000436 DAELJO010000000
VERSION     DAELJO010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.439"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>819
                     /locus_tag="KPB88_004073"
     CDS             <1..>819
                     /locus_tag="KPB88_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0056251.1"
                     /translation="IEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNNNYEELTSKE
                     SVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEINSQATFDLIV
                     VKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIES
                     YDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDI
                     EKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYF"
BASE COUNT          344 a           69 c          113 g          293 t
ORIGIN      
        1 tattgaaatg tattataaaa atcgaaatag ttatatagaa gatagaacgt ctgtagatat
       61 tattagagaa agtgatttta aaaaattaag acaatatcag aataacaatt atgaagaatt
      121 aacttcaaag gaatctgtgt actatgtttc tgattcatac aaaaaaatat tttttaaaga
      181 ctttgagatt gaaaaaataa acttgcatat taaagatgca aatgagtatg atataaatct
      241 gtttaaagta caaaaaagca tcttggagcc agaaataaat tcacaagcta cgtttgattt
      301 aattgtagta aaagatgacg tatttaatca gttaaaatta catggaaatt ctagattaat
      361 aagattaatt gatataaaaa atgaaaaaaa agaactggat ttgactttaa aattaaaaaa
      421 tatagtaagt gaaaatatga aatttactta tccttttaat tttacatcaa gtattgaaag
      481 ttatgataac ttgataaaat taagtggtct aatgctgttt ataggcatat ttttgtctgt
      541 agtatttctt ttatgtacag gtagtataat cttatttaaa caattatcaa atatatatga
      601 tgataaggaa agatatatta tgttaataaa acttggagca aataataaag atattgagaa
      661 aataatatca aaacagttaa aagttatatt tttaatgcca ttagttgtag gtacagttca
      721 taatttattt gctatgtcta tagcacaaaa gtttatacca aggtcattac ttgtaccaat
      781 tattataact ctagtcattt attttatagg atattttat
//
LOCUS       DAELJO010000437          814 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.440, whole genome shotgun sequence.
ACCESSION   DAELJO010000437 DAELJO010000000
VERSION     DAELJO010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.440"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..>814)
                     /locus_tag="KPB88_004074"
     CDS             complement(<1..>814)
                     /locus_tag="KPB88_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0056252.1"
                     /translation="YGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEG
                     RNNDKYNFYSSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKI
                     TSLYIFKNFKIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFL
                     CEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIY
                     GIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLI"
BASE COUNT          369 a          101 c           76 g          268 t
ORIGIN      
        1 aatatcaatc tttgtataat actatggtaa ttaataatta tttcagaatc attgaaaatt
       61 aatcctaagc tattaattag aacatgcatt atagagcaaa accatatgtt cttagttttc
      121 atataaacat aacctaaaaa tatagaaagt ccaataccat atatcaaatg ggctatagct
      181 ccaattattg gagtttgagg actatatagt gtaaattgta aaggtaagtg ccataaactc
      241 caacaagtgc ctaccattac aatccctatt tttttgccaa atctatcaaa taaaatactt
      301 tgcaaaaaag atctccaacc atactcttca cataaaaaag gataaatgta tactaatgat
      361 attataggaa gaagaatata attcaagaaa ttcttataat caaattgatt atcacttatt
      421 attaatccaa aaataatttg agaaaataaa aagtaaaaaa atagcaaaat acctatctta
      481 aaatttttaa aaatatataa acttgttatt ttataagaat aaacaataac acataaacta
      541 cttattaaaa ttaacataag atacattaat tgaaattgat taatacttat taactttaat
      601 gattcaataa taaatataaa aaagtaaata ataaaaaata atattactga tgaataaaaa
      661 ttatatttat cattatttct tccctctgtg taaaattttg caatagaaac tgaagataat
      721 ggcaatatca tcataaattg agctaaaatt tctgaatcta ttaaatgatt aaaacatatt
      781 gctaataaac ctagtgaata agataaacca taag
//
LOCUS       DAELJO010000438          791 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.441, whole genome shotgun sequence.
ACCESSION   DAELJO010000438 DAELJO010000000
VERSION     DAELJO010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.441"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(85..>791)
                     /locus_tag="KPB88_004075"
     CDS             complement(85..>791)
                     /locus_tag="KPB88_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056253.1"
                     /translation="KVNNICILKIDIKNKSYKLLNRFNLGLQNGEQQNINEIKFSIGS
                     KLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGL
                     LSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSG
                     VRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAEL
                     INGDISLQEKLFFVAN"
BASE COUNT          266 a          124 c           57 g          344 t
ORIGIN      
        1 aaaagtttat tttgagacac tcccttactt atacttatct gtgatacata taaaagttta
       61 aagttctaaa attaattcta acacttaatt tgcaacaaaa aataactttt cttgtaaact
      121 tatatctcca tttattagct cagcttcata aatattcttt ttaagtttca tatcaaatac
      181 ttgaaatttt actggcttag ttccgactac atcaacaatc cctttaaatc cttttaattc
      241 tcctgatata ctgttcttaa tacctgaaat agaatatatc ttatcatcca gtagcttaac
      301 tttcctaact ccagatgatg cttcattata atctactttt aaatcaagta tctcataatc
      361 ttctaattta atctcattag aattaatatc ataaacaaga ttagttatgg aattactcat
      421 atccgtaact ataagattca atttattatt ttttaccata taatctagct tagttttact
      481 taataagcca atctcctttg gatttgcatt cttttctttg tttagattat ccataaattt
      541 attacttata ttaaaagtgt tatcttctat atcatatata ataaagttag agtaactatc
      601 atgtccttca atatatccat ttttactttt attatttact atttctaagt ataatttact
      661 tccaatagaa aatttaattt cattgatatt ttgttgttct ccattctgta atcctaaatt
      721 aaatctattt aataatttat aagacttatt ttttatatct atctttaata tacaaatatt
      781 attgacttta g
//
LOCUS       DAELJO010000439          791 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.442, whole genome shotgun sequence.
ACCESSION   DAELJO010000439 DAELJO010000000
VERSION     DAELJO010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.442"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            286..756
                     /locus_tag="KPB88_004076"
     CDS             286..756
                     /locus_tag="KPB88_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0056254.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          360 a           54 c          151 g          226 t
ORIGIN      
        1 gatagaaagt actgaataga tagagataaa agtgttaatg gaagatattg aaaaaataaa
       61 agattgatag aaagtactga atagatagag ataaaagtgt taatagaaga tattgaaaaa
      121 ataaaagatt gatataagat actgaagagg tagaaacaaa agtgttaata gaagatatta
      181 aaaaagtaaa aataaaggat taataaaagg tactgaagag atagagaaaa aaatgttaat
      241 agaagatact gaaaaaatag aaataaaaaa ttaggaggaa aatttatgaa atatcaggtt
      301 tcattattag cagtgaagga tattgaagta tcaaaaaaat tttacaaaga attatttgag
      361 caggaaattg aattggactt aggtaaaaat gtaacattta aaggaggatt tgctatacaa
      421 gaagacttcg catggcttac agggattaat ccagatacaa ttatacaaaa atcaaataat
      481 atggaacttt attttgaggt tgatgatttt gacgaattta ttgaaaaact caattattat
      541 aaagatgtag agtttgtcca taagccacta aaacatgaat ggcaacaaag agtagtgcgt
      601 atttatgacc cagataagca tattattgag ataggagagt ctatggaagt tatagctaga
      661 agatatttat ctgagggata ttctgtagaa gaaacttcta aaataattca acatccaatt
      721 gaatttgtaa agcagtgtga aaatagtaag gaatgattgt ttaaattata attatttagt
      781 agaaattaat t
//
LOCUS       DAELJO010000440          768 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.443, whole genome shotgun sequence.
ACCESSION   DAELJO010000440 DAELJO010000000
VERSION     DAELJO010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.443"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(287..766)
                     /locus_tag="KPB88_004077"
     CDS             complement(287..766)
                     /locus_tag="KPB88_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0056255.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          308 a          111 c           56 g          293 t
ORIGIN      
        1 tttatactga ttatttacaa atatatctaa attaattata aattttattg aataagactt
       61 ttataattat acctttcata aattataaaa aagcactttt ataaaaaatg tgcttttaat
      121 atctactaat aaagttcttt attttaatta aaacataatt atattttagt attataattt
      181 tttaatttta gcattatagt ttatgtatta taccctaata tattttaaat tactagttaa
      241 tcactctttt cgggttattt atctcctata tattaaagtg tcacacctaa atagttaaat
      301 cattatcctc ttttatcttt attgccttta caaatccatc tgcaattaca aaactcataa
      361 gagatgttat aaaatataca aatactggaa cagtaaaata aaatccaaat cctaaatcta
      421 gaaatctcat tccttttcca gtagtaccaa aagttgaaat aaactcaatt agagagttta
      481 aaagcaaaag atatccaata atcctaaatc tttttacatt ctcttttaca aataggctat
      541 attcagaact acctaatact tctatcaatg attttataat gataaaatat gatacaccta
      601 aaataaacat taatattcca taaatagtac cttgtagagt tgcttctcca ctaaaacaga
      661 ggtacataaa acttttaaat cctcctgcaa caattactaa tcctaaaaga aaaaataaaa
      721 tttgtaatat accacttagt aaatttttta taagtttatt agacatca
//
LOCUS       DAELJO010000441          762 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.444, whole genome shotgun sequence.
ACCESSION   DAELJO010000441 DAELJO010000000
VERSION     DAELJO010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.444"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            197..>762
                     /locus_tag="KPB88_004078"
     CDS             197..>762
                     /locus_tag="KPB88_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG0056256.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     NELFNIISIEDIESYIISNIDLKNLKII"
BASE COUNT          335 a           53 c          115 g          259 t
ORIGIN      
        1 ataaaaaata tattatgtta tttatttgaa tagattatcc agaagacata aataagaaaa
       61 ataatgtgta agtaaaaata ttagaattta ttaaaagcta ttacaagtat aagaaatttg
      121 aaatgattat tcaaaaattg acatgtgaaa aaaaatatga tatgctgcaa taaaagttaa
      181 tgtgaggtat gtcagaatgt ataatgttat agatcttttt gcaggtgcag gtggtttgag
      241 cttaggattt gaaatgacta aaaaatttaa tatggtggct tttgtagaaa aaaatgataa
      301 cgcagcaaaa acataccttg aaaatcatcc gagtgttaag cgttattgtg atattaaaag
      361 attagatttt caagatatat taaacagtgt cgataagata gatgttgtta taggaggacc
      421 gccatgtcaa ggtttttcta atgctaatag acaaaaaaga aaaattataa atggaaataa
      481 tgaattagtt aagctatatg ttgatgctat tgataaatta aagccaaatg tatttgtaat
      541 ggaaaatgtc aaaactatat cttcaaataa gcattcattt tatttgacta aaaaagataa
      601 aaatcatata ataaataatt taaaattaaa tatttataat aaagatagtg ttttatatga
      661 tagaactgag tatataaatg aattatttaa tattattagt atagaagata ttgaatcata
      721 tattatatca aatatagact taaaaaattt gaaaataatt at
//
LOCUS       DAELJO010000442          743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.445, whole genome shotgun sequence.
ACCESSION   DAELJO010000442 DAELJO010000000
VERSION     DAELJO010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.445"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>743
                     /locus_tag="KPB88_004079"
     rRNA            <1..>743
                     /locus_tag="KPB88_004079"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          153 c          226 g          159 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gat
//
LOCUS       DAELJO010000443          731 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.446, whole genome shotgun sequence.
ACCESSION   DAELJO010000443 DAELJO010000000
VERSION     DAELJO010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.446"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            162..500
                     /locus_tag="KPB88_004080"
     CDS             162..500
                     /locus_tag="KPB88_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056257.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            522..>731
                     /locus_tag="KPB88_004081"
     CDS             522..>731
                     /locus_tag="KPB88_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0056258.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTG"
BASE COUNT          322 a           80 c          111 g          218 t
ORIGIN      
        1 tttatccata tttgtttatt gtagatacag actatacaaa aataaatagc acttatttca
       61 gtataaaaac atatatagtc tatatttaga ctaaaaaagt ataaatagtc tatataaaac
      121 aacttaaact atgaatataa tctaaagtgg agggataata attggcaaat ataggaaaaa
      181 taataggaga tagaataaaa gtctatagaa caaaattggg atatagtcaa gagtttctag
      241 cagaaaaggc aggtttacat ccaacatata ttggtcagat tgaaagagct gagacaaata
      301 catctatcaa tattataatg aaaattgcta ctgctttaga cataccactt gaattgttat
      361 ttgcaaatat tattacaaca gaaggcgttg ataactctgt acctttagaa tgttatgaaa
      421 tgattaataa attaactgaa aaagaacaaa ttgcaatgct agagttaata aagtgcatca
      481 ttaaatacaa agaaatataa gtgagggaaa tagtaacaat aatgaattat ataggtaaaa
      541 gacttaaaga agaacggaaa aaagcaaatt taacttcaaa agaatttgca aatatggttg
      601 gagtatcgcc ttggtatata actcaaatcg aaagtggtaa aaagaatcca agtcttaaaa
      661 cattcataaa atttgtaaat atattaaata tttctgcaga tgttcttata aaagatatta
      721 cttccactgg a
//
LOCUS       DAELJO010000444          716 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.447, whole genome shotgun sequence.
ACCESSION   DAELJO010000444 DAELJO010000000
VERSION     DAELJO010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.447"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            50..516
                     /locus_tag="KPB88_004082"
                     /pseudo
     CDS             50..516
                     /locus_tag="KPB88_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          264 a           37 c          143 g          272 t
ORIGIN      
        1 attctaatta tatattataa ataaagtatg tgtatattaa ggaggtaata tgaagttttt
       61 aaatgtttta atagtagttg aagatattga gaaatcaaaa aagttttatt atgatgtttt
      121 ggggttaaag gtaatctgtg attttggaga aaatgttgta ttagaaggta atatatcatt
      181 acaagaaaaa aagctttggt tagaatttat taataagagt gacagtgaag ttaagttcaa
      241 tggaaatgat gctgagttat attttgagga agataatttt gatacttttg ttgaaagatt
      301 gagttcaatg aaaggtatag attatgttca tctagctata gagcataggt ggggtcaaag
      361 agcaattaga ttttatgatt tagatgggca tattattgag gttggagaaa ctatgtcact
      421 gtgtgtagac gttttttaga tagtggtctt tcaatggatg aagtagctaa aagaatggat
      481 gtaactgtcg aatatattga atcagtttta gaatagatgg attatattaa agtaaaattg
      541 aggtgaaggc tgtaagtaaa cttgtgtgaa aacaaatttg cttacagttt ttatttttct
      601 aaaataaaga atataatatt tgtttagctt tatttgagtt tttaaaaata ttatgatatt
      661 tcttatatat ttatactaaa gtttagattt agaaaaaata tataaaagat agttat
//
LOCUS       DAELJO010000445          703 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.448, whole genome shotgun sequence.
ACCESSION   DAELJO010000445 DAELJO010000000
VERSION     DAELJO010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.448"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>703
                     /locus_tag="KPB88_004083"
     CDS             <1..>703
                     /locus_tag="KPB88_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0056259.1"
                     /translation="ITNNTKNDEELNDKIITIQGVVKGKIEKKRYNQYKVGVFLINDY
                     TKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGRYIKSTGYKGIVYINNYKIIDKNKLY
                     SLIGEIKFYISKTYRYLYKKNSDFINSILLAEVENLTEEQKEIFTRTGTSHVISISGL
                     HTGILCVIISFLLRGINKLYKLLILSIFITLYCIMVGASPSIIRSIAFVMIFYLSIFI
                     DRKKDGISALSLIGI"
BASE COUNT          295 a           59 c          109 g          240 t
ORIGIN      
        1 ttattacaaa taacacaaaa aacgacgaag aacttaatga taagataata actatacaag
       61 gagttgtcaa aggaaaaatt gaaaagaaaa gatacaatca gtacaaggta ggggtttttc
      121 taataaatga ttatacaaaa aataaaaatt tgaagattgg tcagaaagtt aatattacag
      181 gtaaattcaa aagtttagat aaaatgaagt atgatgattt tgattatgga aggtatataa
      241 aaagtactgg atataaaggt atagtgtata taaataacta taaaattatt gataaaaata
      301 aactttactc tttaataggg gaaataaagt tttatattag taaaacttat aggtacttat
      361 ataagaaaaa ctcagatttt ataaattcta tactattagc agaagttgaa aatttgactg
      421 aggaacaaaa agaaattttt actagaactg gaactagtca tgtaatatct atatcaggtc
      481 ttcatacagg aatattatgt gtaattatat cctttctatt aagaggtata aataaattgt
      541 ataaattatt aatactgtcg atttttataa ctttatattg tataatggta ggtgcgtctc
      601 catccataat taggtctata gcatttgtta tgatttttta tttatcaata tttattgata
      661 gaaagaaaga tggaatatcg gcattgtctc taattgggat aat
//
LOCUS       DAELJO010000446          698 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.449, whole genome shotgun sequence.
ACCESSION   DAELJO010000446 DAELJO010000000
VERSION     DAELJO010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.449"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
BASE COUNT          281 a           47 c           84 g          286 t
ORIGIN      
        1 ataaggtggt aaatttatat aaaaaagaat atgagttgta aaatattaaa taatattgta
       61 ctaattggaa taattttaac tttattatag attattttaa taccattagt actgatggtg
      121 tattttaaaa ttggtataag cattacaaat acgaatatgc caactgtaat atgtaatata
      181 agtagctata tacatatgtg ctgtcccata tattgtatca ttgtttatat taaaaagatt
      241 atgtaaattg gtatcaatca ataaatcatt ttcagaagaa atacctaaaa acttaaaaaa
      301 tacctatatg tgtatttagt tagatttgag atttttgcat ttaattttat agtgacaata
      361 ttatattatt tttatgatgt ctatttatat gcaattacta taatatcaga acttatagta
      421 tcatttgttt cattatcaat aaggtttttt actttagttt tgtcaatttt attaaaaaaa
      481 gttattgata taaaagataa gaaggataaa actatatgaa ggatttcaaa cataagagtg
      541 gatatttata taatagatat tttatagtta attttattag attttattta atactgtaat
      601 attaatatca caatatatta atagtgtatt aaagattgaa ttttgtagat aactataatt
      661 tattgagagt gataaataaa aattatataa tattacag
//
LOCUS       DAELJO010000447          691 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.450, whole genome shotgun sequence.
ACCESSION   DAELJO010000447 DAELJO010000000
VERSION     DAELJO010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.450"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>691
                     /locus_tag="KPB88_004084"
     CDS             <1..>691
                     /locus_tag="KPB88_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0056260.1"
                     /translation="EIAKKLPDNIAKFFMDSHNIQLDWEKNYPYDKISNWIFEFKKDT
                     TSYWNVLEYKVDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRF
                     DIFIGDYIVLIDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFI
                     VIFSSDVDKNYVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMY
                     KNKEKNKKKFD"
BASE COUNT          291 a           66 c          100 g          234 t
ORIGIN      
        1 tagaaatagc taagaaactc cctgataata ttgctaaatt ttttatggat tctcacaata
       61 ttcaattaga ttgggaaaaa aattatcctt atgataaaat ttcaaattgg atttttgagt
      121 ttaaaaaaga tactacaagc tattggaatg ttttagaata caaagtagat gttgatgaaa
      181 aaacctacat aattactatg gccaatgata taacaaaact ctatgaagaa aataagagaa
      241 atcttcacta ctcaattact gacccattaa ctggagcata taatagaaaa tatttaaatg
      301 atagatttga catatttata ggagattata ttgtactaat tgacttagat aatttcaaaa
      361 tgataaatga ctatgaaggt cataatgtag gagacaagat tttatgtgat tttgtttcac
      421 ttttaaataa agaattaata aattctactt ctattataag attaggagga gatgaattta
      481 ttgttatatt ctctagtgat gttgacaaaa attatgttta ttcacaacta gaagccttaa
      541 gagaaaattt tttaaaagta ttctctaagt ataagtatct ttcatttagt tatggtgttg
      601 atacagtaaa aagaaattta aaactgacaa tagttgaatt ggataaaaaa atgtataaaa
      661 acaaggagaa aaacaagaaa aaatttgata a
//
LOCUS       DAELJO010000448          690 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.451, whole genome shotgun sequence.
ACCESSION   DAELJO010000448 DAELJO010000000
VERSION     DAELJO010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.451"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            223..591
                     /locus_tag="KPB88_004085"
     CDS             223..591
                     /locus_tag="KPB88_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG0056261.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          299 a           54 c          111 g          226 t
ORIGIN      
        1 aaaatagagc taatttaata tataataaaa gcatatcaag taaagcttta aattaaagta
       61 attttatata aaaagtaata taaattgata aaattataaa tttatttata gtgaatatag
      121 gttggatttt atagatacaa aaatagtata tatgatatgt agaccattag catgtttaaa
      181 gtagcaaaat atataaaata aaatttgaaa ggaagtataa aaatgacaag accatcaata
      241 tatcatagcc aaggatacac ttgtgcagag gcattaataa agtcttataa tgaggaacac
      301 aacacagata taccaatctc aataggaagt ggtatggggg ttggaatgaa tgttggaagt
      361 gtttgtggag ctgtaaatgc tgctgttgtt ataatagggt atttaaatgg gcgaaatagt
      421 aatttagatg aaaatatagc aaggaagtat tcaagagagc tattgaatag agtaagaaat
      481 aaatacagct cagaaatatg tgtatctctt aaaaaaaata aagtaacttg tgaagaaata
      541 ataaatttca catatgatgc tctaaatgat atgctaaaaa atcaaaaata attctaagaa
      601 acaagtattt tacattcgat agaatacttg tttttttatg atatttttaa taggtattag
      661 tttattataa tttttaaatc aattttatta
//
LOCUS       DAELJO010000449          664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.452, whole genome shotgun sequence.
ACCESSION   DAELJO010000449 DAELJO010000000
VERSION     DAELJO010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.452"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..>664)
                     /locus_tag="KPB88_004086"
     rRNA            complement(<1..>664)
                     /locus_tag="KPB88_004086"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAELJO010000450          647 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.453, whole genome shotgun sequence.
ACCESSION   DAELJO010000450 DAELJO010000000
VERSION     DAELJO010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.453"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>647
                     /locus_tag="KPB88_004087"
     CDS             <1..>647
                     /locus_tag="KPB88_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0056262.1"
                     /translation="RIEVYKNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALK
                     DLDNAYKKFFKKKAGFPKFKSKKINRFSYKTNFTNGNIVYCGQHIKLPKLGMVKIRDK
                     QVPKGRILNATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEF
                     IENPKYLKKSLNKFAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQK"
BASE COUNT          287 a           71 c           96 g          193 t
ORIGIN      
        1 aaagaataga agtttataaa aacgataaag aaacttttac ttataaacag tgtagttctg
       61 atttgaccaa tttaaaaaaa gaattaaatt ggcttaagga acctgacaaa ttttcactac
      121 aaaacgcctt aaaagattta gataatgcat ataaaaaatt ctttaaaaaa aaggcaggat
      181 ttcctaaatt taaatcaaag aaaattaata gattttcata taaaactaac tttacaaatg
      241 gaaatatcgt gtattgtggt caacatataa aactacctaa acttggtatg gttaagataa
      301 gggataaaca agtacctaaa ggtagaatac ttaatgctac tatatctaaa gagccaagtg
      361 gtagatatta tgtatcatta tgctgtactg atgtagatat tgaagcattt gaaaatacta
      421 ataatcatat aggtttagat ttaggaatta aagaattttg tatctcaagt tgtggagaat
      481 ttatagaaaa tcctaagtat cttaagaagt cattaaacaa atttgctaaa ttacaaagag
      541 aattatcgag aaaaacaatc ggtagcttaa atagaaataa agcaagatta aaagttgcaa
      601 gacttcaaga acatatagct aatcaaagaa aggattttct acaaaaa
//
LOCUS       DAELJO010000451          575 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.454, whole genome shotgun sequence.
ACCESSION   DAELJO010000451 DAELJO010000000
VERSION     DAELJO010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.454"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            35..>575
                     /locus_tag="KPB88_004088"
     CDS             35..>575
                     /locus_tag="KPB88_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0056263.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDV"
BASE COUNT          245 a           60 c           97 g          173 t
ORIGIN      
        1 tactataata ctatattgcg aaaagaggta gaaaatgttt gcagaagaaa gaatccaagc
       61 tatattaaat atcctaaaaa aagaaggtag agttactgtc aaagagctca gttctaaatt
      121 taatgttaca gaagattgta taagaaaaga tttaaaaaat atagaaaaga tttcttcaat
      181 aagaagaatt tatggaggag ctgtactagc tagagaaaca ctagagaatc aggatacaaa
      241 agacagaaaa gaaattaata ttccaacaaa gaaaataata gcagaaaaag catttaattt
      301 aattgaagat agagaaacta tatttttaga tatctctaca attaacatct tgcttgcaaa
      361 gttgttagct gaaagtaata aaaaagtaac acttgttaca aatatgttgg atatactaaa
      421 tgtagttact tcaaaaccaa ataatttaaa tataatttcc actggaggtt tattaaatct
      481 cagtttagac ggttttgtcg gtactccaac tatagagttt atatcgaaat ataagtttga
      541 taagactttt atgggaagtt gtggagtaga tgtat
//
LOCUS       DAELJO010000452          567 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.455, whole genome shotgun sequence.
ACCESSION   DAELJO010000452 DAELJO010000000
VERSION     DAELJO010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.455"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>567
                     /locus_tag="KPB88_004089"
     CDS             <1..>567
                     /locus_tag="KPB88_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0056264.1"
                     /translation="DLEIFRNFKFSLNLICALTSFICIASSSILIPFYLQSTMKLPPI
                     QAGLFMILSPLILAIFSPIFGNISDKIKSEKIILIGLLVMSFGFFLMSRLKESSALIL
                     FVIYISIISIGQAIFQPANNALIMSSCSRSKLGVVGSINSLVRNLGQVIGITISTTLL
                     YNFMSIKAGYRVNDYVINNDKIFVFGMR"
BASE COUNT          187 a           70 c           81 g          229 t
ORIGIN      
        1 ttgaccttga aatttttaga aattttaaat tttcattgaa tttaatttgt gctcttacat
       61 cattcatttg tattgcatca tcaagtattt taattccatt ttatttacaa agtactatga
      121 aacttccccc tattcaagca ggtttattta tgatactatc acctttgatt ttagccattt
      181 tttcacctat atttgggaat atatcagata aaattaagtc agaaaaaatt attttaattg
      241 gtcttctcgt tatgagtttt ggattctttc taatgtcaag attgaaagaa agttctgccc
      301 ttattttatt tgtaatttat atatcgataa tatcaatagg acaagctata tttcaacctg
      361 ctaacaatgc tttgataatg tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca
      421 taaattccct tgtaagaaat ttaggacaag ttataggtat tactatttct acaacacttt
      481 tatataattt tatgagtatt aaagctggat atagggtcaa tgattatgtg attaataatg
      541 ataaaatatt tgtatttgga atgagaa
//
LOCUS       DAELJO010000453          552 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.456, whole genome shotgun sequence.
ACCESSION   DAELJO010000453 DAELJO010000000
VERSION     DAELJO010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.456"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>552
                     /locus_tag="KPB88_004090"
     CDS             <1..>552
                     /locus_tag="KPB88_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0056265.1"
                     /translation="SSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLYIN
                     IGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLD
                     NMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFF
                     VLYDYIINLVKISPERRTAFLVIV"
BASE COUNT          192 a           49 c           77 g          234 t
ORIGIN      
        1 ttcttctatt atagaaatat ggtcatgtaa aaaagttttt gattatacta gtcagtcaaa
       61 gaccgactta aaaaaactaa atatgtattt tctcttttct gtttttctca ttatttcttc
      121 tttatatata aatataggag ctaatagtag aatcttgata tgtatatttg taatgttcgt
      181 tttctataag tctaactata aggttaatat atataaatgt ctggtaatct cattttttta
      241 ttggttaata ttaataggta taaatggatt aagtatgtta ttagtagcaa cagtaagcaa
      301 tttggataat atgaatgttt tacttagtgg cagtttatat agatatgaag caatattctt
      361 gggaaagata atgttaggta tattacttta tgtttataag gcttctttta aaaattttaa
      421 aataagtaaa aaagagatat acatatgtat tcctatgata gcaaatttat tgtcattttt
      481 tgtactttat gattatatta ttaatcttgt aaaaatctct cctgaaagaa gaactgcttt
      541 tttagtaata gt
//
LOCUS       DAELJO010000454          548 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.457, whole genome shotgun sequence.
ACCESSION   DAELJO010000454 DAELJO010000000
VERSION     DAELJO010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.457"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            47..>548
                     /locus_tag="KPB88_004091"
     CDS             47..>548
                     /locus_tag="KPB88_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG0056266.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDY"
BASE COUNT          231 a           73 c           69 g          175 t
ORIGIN      
        1 aaactcaatt atataatttt taataatatt attttaggag gtaaaaatga taaacgatat
       61 taaaaataaa ttcaaaaatt acaaaccata tataaatgga tacaagaata tgaagagagc
      121 atctgtttta attccaatag tagaaataaa taacacacac tatatattat ttgaagttcg
      181 ctcaaaaaat ttgaagcatc aacctagcga aatttctttt ccaggtggta aaatagaatc
      241 aggtgagact ccatatgaag ctgttattag agaaacttgt gaggaacttg gaacttttag
      301 tgacaatata gaagtaatat caagtcttga cctccttata acaccagtaa agtttatcat
      361 acatccatac ttaggatata taaaaaatat taataattta aatataaaca aggatgaagt
      421 tgaccatact tttttagttc ctataaaata tcttttagag aatcctccaa atacttatag
      481 taatactgta aaagtttttc cagatgaaaa tctaccatat ggttcaattc caaaccaaaa
      541 agattata
//
LOCUS       DAELJO010000455          547 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.458, whole genome shotgun sequence.
ACCESSION   DAELJO010000455 DAELJO010000000
VERSION     DAELJO010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.458"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(<1..>547)
                     /locus_tag="KPB88_004092"
     CDS             complement(<1..>547)
                     /locus_tag="KPB88_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBG0056267.1"
                     /translation="NGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDF
                     SISLVSKNQVKVNGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFE
                     NINFVIDKYFTLVGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVE
                     PIYNPDTGEDISTSLDFSYEPL"
BASE COUNT          202 a           83 c           56 g          206 t
ORIGIN      
        1 agaggttcat aggaaaaatc tagtgaagta gatatatctt cacccgtatc aggattatat
       61 ataggctcta ctacaacatt tctaccattt ccgctaaata tagtgctttt agatgacgat
      121 gttttccatt caccaaaata tatatctata tttttattat tgtcacaaat aaattctaca
      181 tatccaagat tagttttacc aacaagggta aagtatttat cgattacaaa atttatattt
      241 tcaaacccaa acaatttcca gaaacttata ttgtccaaaa ataaattcat aaaattagaa
      301 gtattatggt gtccatctga atttttcaca aaatcaaggt aagatgagta tacggattca
      361 tttaaatata atccatttac ttttacttga tttttactaa ctaaagaaat agagaaatct
      421 atacttttat cagtattatt atcaacatag tattttcctg ttatagtatt aatatcatct
      481 ttcataaata catttatatc ttcagcaata aaatctttac tgttaaattc taatgtactg
      541 ccattat
//
LOCUS       DAELJO010000456          543 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.459, whole genome shotgun sequence.
ACCESSION   DAELJO010000456 DAELJO010000000
VERSION     DAELJO010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.459"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
BASE COUNT          215 a           43 c           74 g          211 t
ORIGIN      
        1 atttagtttt attctactat taccataata taggaaaacg tttctttttt caaggtgcat
       61 attaaaaaat ttaaaaatta aaaaaatatt ttatttctca aaaataaatt ggataaaata
      121 attatattat agaatttgat tcttatctaa aatgttggtt gctgaggatt ttgatgataa
      181 tgacgagtaa tttttatatt atcacaaaag gtacttatta aaaggcttgt atcttaaaaa
      241 tctagataga ggtttatttt taagattact gaatattatt actgaattta ttgaaggatt
      301 ttttgtagat aaagataagt agagtctaaa agctatttct aatctgtaat ctgtaacgat
      361 tgcaaattag attagtgaaa acagaaattt agcacaatat ttgtatggaa ttgatataca
      421 tccaaatatg cttgaaatag caaaataaaa actagaaaat tcagttaatc tagttttgga
      481 agatgttgat aaattaccat tttaagataa tttatttggt gtaataatgt acaatttata
      541 tta
//
LOCUS       DAELJO010000457          531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.460, whole genome shotgun sequence.
ACCESSION   DAELJO010000457 DAELJO010000000
VERSION     DAELJO010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.460"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..>531
                     /locus_tag="KPB88_004093"
     rRNA            <1..>531
                     /locus_tag="KPB88_004093"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          144 a          112 c          175 g          100 t
ORIGIN      
        1 accaaggcga cgatcagtag ccgacctgag agggtgatcg gccacattgg aactgagaca
       61 cggtccaaac tcctacggga ggcagcagtg gggaatattg cacaatgggc gaaagcctga
      121 tgcagcaacg ccgcgtgagt gatgaaggcc ttcgggtcgt aaaactctgt cctcaaggaa
      181 gataatgacg gtacttgagg aggaagcccc ggctaactac gtgccagcag ccgcggtaat
      241 acgtaggggg ctagcgttat ccggatttac tgggcgtaaa gggtgcgtag gcggtctttc
      301 aagtcaggag tgaaaggcta cggctcaacc gtagtaagct cttgaaactg ggagacttga
      361 gtgcaggaga ggagagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga
      421 acaccagttg cgaaggcggc tctctggact gtaactgacg ctgaggcacg aaagcgtggg
      481 gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgagta c
//
LOCUS       DAELJO010000458          530 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.461, whole genome shotgun sequence.
ACCESSION   DAELJO010000458 DAELJO010000000
VERSION     DAELJO010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.461"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
BASE COUNT          199 a           69 c           29 g          233 t
ORIGIN      
        1 cattaattat atacctcaat aaatttctat aaaaatagta tcattttagt atcatcattt
       61 atttaataca ttatgtattc cgtttagatt tagttatccg aaaacttaga taactagctt
      121 gaaatttcta cagattatta catactttat tgtaattata tcataatgtt ataattacat
      181 attatcaaat tacacactat cttttattca tatagatatt atcatccttg tgtatatatt
      241 gtcaaagaaa acatttaatt tacaacatat tttatatcat atcatctaaa ctattatata
      301 caccttatct aattcacaca ttaacactat tgtacttttt ctaaaaattt ttaaataaaa
      361 atgacagctt taatttatta ttttaaaact gccattaaac tgtaaatatt cccttttagt
      421 atacaaaatt ttacctttat gttaatatat tttacttata taaatttttc ataattaatt
      481 tatacatata aattagcacc attgttgttt tattatctta gttataaata
//
LOCUS       DAELJO010000459          511 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.462, whole genome shotgun sequence.
ACCESSION   DAELJO010000459 DAELJO010000000
VERSION     DAELJO010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.462"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            <1..135
                     /locus_tag="KPB88_004094"
     CDS             <1..135
                     /locus_tag="KPB88_004094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0056268.1"
                     /translation="LLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI
                     "
     gene            263..451
                     /locus_tag="KPB88_004095"
     CDS             263..451
                     /locus_tag="KPB88_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056269.1"
                     /translation="MKIIYKNKVYKVEQDKVLFRITYYDEQRGSKKFNKDKKVKRSVL
                     TRDIEVVNLYLPTHLKIK"
BASE COUNT          240 a           43 c           95 g          133 t
ORIGIN      
        1 cttttgaagt cagttgatat aggggcaact aaagtttcta gttatgaatt agatgatttt
       61 ataactagaa atcaaggaaa aaacatcaaa gaaatgctta gagaaatgaa agaaattaga
      121 gagggggtga tttgattgga actagtaaca tacagaaaca agcttgtttt actcaaagat
      181 ggagaaaaga ttgcaactat aagtttaaaa aggaaatttc tcagtaataa aattaaatta
      241 aaaataagat aggagagata aattgaaaat aatctataaa aacaaagtct acaaagtaga
      301 acaagacaaa gtgttattta gaattacata ctatgatgag cagagaggta gtaagaagtt
      361 taataaagat aagaaagtaa aaagaagtgt tttaacaaga gatatagagg tagttaactt
      421 gtatttacca acacatttaa aaataaagta agctataaat aattaaagaa aaaggtgatt
      481 agatggaaat agagcaaaca acaatacgcc t
//
LOCUS       DAELJO010000460          460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.463, whole genome shotgun sequence.
ACCESSION   DAELJO010000460 DAELJO010000000
VERSION     DAELJO010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.463"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            56..277
                     /locus_tag="KPB88_004096"
     CDS             56..277
                     /locus_tag="KPB88_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056270.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          214 a           25 c           84 g          137 t
ORIGIN      
        1 tactttggga aaaataaata taggaagtat tatatttaca gaggaggtag ttttgatgga
       61 tagattaaaa agagatatga aaaatatgaa agaagattta aaagaaggtg ttaaaaagac
      121 agcttatgtt atgaaaaata cagctgaaga tttgaaagat gatgcacaag gtgctatggt
      181 aaacttagaa atgaaaaaag atgagatgat ggaaagatat gaacaaaaga aattttctaa
      241 agaattagag aagcaaatga agaatgaaat gaaataagct tttatttatg aataacaaat
      301 tattgtattc atataaaaag tagccagtaa tattaaatag tttatttaag gaaatagact
      361 ttctaaaaat aactgtatta gatttagaaa gtctatttca aattagtttt ataaatagac
      421 ataaggataa agtagatgtt atagaactag attttaaaaa
//
LOCUS       DAELJO010000461          445 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.464, whole genome shotgun sequence.
ACCESSION   DAELJO010000461 DAELJO010000000
VERSION     DAELJO010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.464"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            70..306
                     /locus_tag="KPB88_004097"
     CDS             70..306
                     /locus_tag="KPB88_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056271.1"
                     /translation="MNNMENKKNFLRKIVKLRSIFLSIFGVFLFITATTGIQTVDRYV
                     PTWLYITLGVSATISLVLYVISFILKINIKDSKM"
BASE COUNT          193 a           36 c           55 g          161 t
ORIGIN      
        1 atagattaaa tctgatatta ttatatacat ctggaagttg ttgctattat ttaaaaaggt
       61 gggatggata tgaataacat ggaaaataag aaaaattttt taagaaaaat agtaaaatta
      121 agaagcatat ttctaagcat atttggtgtg tttttattta ttacagcaac tacaggtatt
      181 caaactgttg atagatatgt tccaacatgg ttatatataa cattgggagt gtcagcaact
      241 atatcattag ttttatacgt tatttccttt attttaaaaa taaatataaa agatagtaaa
      301 atgtagtact tcataaactt ccagtaaaag aataattttg ataaatcaaa attattcttt
      361 tttaatatca aaataaataa taaaataaca aaataaatag aaaatataac taaaaaatta
      421 tcaaagttta agatgataat tttat
//
LOCUS       DAELJO010000462          439 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.465, whole genome shotgun sequence.
ACCESSION   DAELJO010000462 DAELJO010000000
VERSION     DAELJO010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.465"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            170..376
                     /locus_tag="KPB88_004098"
     CDS             170..376
                     /locus_tag="KPB88_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056272.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTAIFDKIG"
BASE COUNT          195 a           26 c           64 g          154 t
ORIGIN      
        1 ctataattga tttttattta atctgaaaaa aatattatgg ctataaaaaa ttgctactta
       61 ttatttggat atttttgtat ataaataata agtagcatga ctttatacat taataatgat
      121 agaatataaa ttaataggta agatatgatt ttaaaaggag tgatataaga tgagtaaata
      181 tagttcttta tgggaatatg ttaagaaaaa caatagtcaa tcgtttaaat taacttttga
      241 agaaataaaa gatattgctg ggatagagat agaccattcg tttttgaaat ataaaaagga
      301 attaaatgaa tatggatatc aagtaggaaa aatctcttta aaagagaaga ctgctatttt
      361 cgataaaatt ggttaagaca taaataatta catataataa aataatttgt attgatgatt
      421 attacaaata aaaataaag
//
LOCUS       DAELJO010000463          417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.466, whole genome shotgun sequence.
ACCESSION   DAELJO010000463 DAELJO010000000
VERSION     DAELJO010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.466"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     regulatory      156..246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          171 a           39 c           59 g          148 t
ORIGIN      
        1 aattaaagat attttcagag agtttattat aaaattctag agtatagtgt ttataatctt
       61 tgtaattatt gctaactaaa tataaaatat aaaatatttg attatacaca aaaaagatat
      121 ttaaaaaatt tgttatttat tgtatactta agttaaaact aaataggcaa atctagagaa
      181 atctagtgac gcaaagctat agggactaag gtttgtatac acaagctatg taagccagtt
      241 gccaaaaaga gttctaagta tattgtatac gtagaaagag tctaggatga ctgccttttt
      301 ggtggtcatt ttgttatgga aaaaattact ttattgcggt aaaaaatacc tataataaca
      361 tattttacca aatataattt aaaataaaga taaattatct agaaaagtta ctatttt
//
LOCUS       DAELJO010000464          417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.467, whole genome shotgun sequence.
ACCESSION   DAELJO010000464 DAELJO010000000
VERSION     DAELJO010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.467"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
BASE COUNT          132 a           60 c           26 g          199 t
ORIGIN      
        1 catataatct ttttaattca atttaaattt taaatatttt gtgtttatta tataatctga
       61 aaaaaaagca gtcaatagca tttttaattt ttttaatttt caaaattatg attcttaaaa
      121 aatcgttttc cttttatcat aacacctatt tacgttaatt cttgtagtat tttgtattta
      181 ttttgctaaa ttgacagtta actttattca atttttattc tacttatgta gatttacctc
      241 ttgtttttta ctcttaaaac cattttttct gaaaattcct tattatgatt tatcaactta
      301 tttttcttca taacaattac tctctaactt ccaacttact taaactttgc agttgctagt
      361 attcacgttt acatacttat atgtcataat taactaaaaa ttcttaatat atctctc
//
LOCUS       DAELJO010000465          417 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.468, whole genome shotgun sequence.
ACCESSION   DAELJO010000465 DAELJO010000000
VERSION     DAELJO010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.468"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            92..>417
                     /locus_tag="KPB88_004099"
     CDS             92..>417
                     /locus_tag="KPB88_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0056273.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKL"
BASE COUNT          187 a           26 c           69 g          135 t
ORIGIN      
        1 ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa acactaaaat
       61 taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta ttaatgttga
      121 atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag aaaagcaaga
      181 gtatgaagta aagatttata ctgaaagtgg taattatgaa gttggagaaa atgctccttg
      241 tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt atattgtaaa
      301 tctagacaag tgtgaatttt atctcagcaa taattgtatt tttgtgaata ataattttat
      361 aagaatttta ggttcagaaa gaaaaaagaa attaaaaaag aaattagaaa aattata
//
LOCUS       DAELJO010000466          406 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.469, whole genome shotgun sequence.
ACCESSION   DAELJO010000466 DAELJO010000000
VERSION     DAELJO010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.469"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
BASE COUNT          137 a           47 c           27 g          195 t
ORIGIN      
        1 ttcaggatga tttttattat aaaatttttt ataaatatat aattttttta tatttatttg
       61 actttctttt aattctttag gatggattta aattttctat aaaaatcata ctattttagc
      121 tgtttacttg cttatttaat agttttattt ctttgattaa atatctttca gaatctaagc
      181 cagatgtctc aaaaaaactg atttataata taatcatttt ttctgctatt cttatttttc
      241 caattttagt ttcatattaa aatatacttt tcatagacac tacattcttt catactcatt
      301 ttcacattta actatacaag tagtcttact catttatatc ctatgcttta ttaatattta
      361 ttaagaaaaa ttcaaagatt taaaaatata tattttctat aaagta
//
LOCUS       DAELJO010000467          378 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.470, whole genome shotgun sequence.
ACCESSION   DAELJO010000467 DAELJO010000000
VERSION     DAELJO010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.470"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            182..298
                     /gene="rrf"
                     /locus_tag="KPB88_004100"
     rRNA            182..298
                     /gene="rrf"
                     /locus_tag="KPB88_004100"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            305..376
                     /locus_tag="KPB88_004101"
     tRNA            305..376
                     /locus_tag="KPB88_004101"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:337..339,aa:Asn,seq:gtt)
BASE COUNT          120 a           60 c           80 g          118 t
ORIGIN      
        1 atttatttga tgttattcat taagatatat gtgtagtttt tagagtgtta actctatgta
       61 aagtactaac ttcaattgat gaagtatata tcgtaaaaac aattactatt tagtacttca
      121 aaactattga catttaaaaa taaagatgtt aatataatac ttgtcttata aataggcaat
      181 atgtggttat aatagcaaag aggatacacc tgttcccatt ccgaacacag aagttaagct
      241 ctttagcgct gatggtactt ggtgggaaac tgcctgggag agtaggacgt agccacgtga
      301 atattccaag gtagctcaat ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
      361 agccccaccc ttggagcc
//
LOCUS       DAELJO010000468          373 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.471, whole genome shotgun sequence.
ACCESSION   DAELJO010000468 DAELJO010000000
VERSION     DAELJO010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.471"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            187..>373
                     /gene="tcdR"
                     /locus_tag="KPB88_004102"
     CDS             187..>373
                     /gene="tcdR"
                     /locus_tag="KPB88_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG0056274.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSS"
BASE COUNT          155 a           26 c           44 g          148 t
ORIGIN      
        1 ctaaaatact ttatttatta gaaaaagatt actaattaat tattaaaatt aatgtattaa
       61 taatgcatat tttcatataa aatttaattt atttgccgat tatataatta taatgactga
      121 tttaattccg atgttgtcaa aattttcaaa taaatcatca ttataaatat aagagaggat
      181 gattttatgc aaaagtcttt ttatgaatta attgttttag caagaaataa ctcagtagat
      241 gatttgcaag aaattttatt tatgtttaag ccattagtaa aaaagcttag tagagtttta
      301 cattatgaag agggagaaac agatttaata atatttttta ttgaattaat aaaaaatatt
      361 aaattaagta gct
//
LOCUS       DAELJO010000469          368 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.denovo.472, whole genome shotgun sequence.
ACCESSION   DAELJO010000469 DAELJO010000000
VERSION     DAELJO010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.denovo.472"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            205..>368
                     /locus_tag="KPB88_004103"
     CDS             205..>368
                     /locus_tag="KPB88_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0056275.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITS"
BASE COUNT          159 a           30 c           63 g          116 t
ORIGIN      
        1 atagaattag cacaaaaaat tgcaaataag agaggtattt cagtagaaga agcttggagt
       61 gaagcagtaa cagaatataa aaataaatac gaacatattg catagatatt taaaaattta
      121 gaatatttgg tagaatataa tgattttaaa tgatactata aattataagg tatatcatta
      181 ttaacataga aggaggatat ctgcttgatt gtaaaaatag ataatacttt agaaaaagaa
      241 ttttggcaat atgtatcaca tgaagaaagt ttaaatttat tcataattgg atacgttgag
      301 aattatggat ttagctctca atatcaagat atatggtctc aggtagaaga tggaaatata
      361 acctctat
//
LOCUS       DAELJO010000470         9271 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.guided.153, whole genome shotgun sequence.
ACCESSION   DAELJO010000470 DAELJO010000000
VERSION     DAELJO010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.guided.153"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            complement(306..1073)
                     /locus_tag="KPB88_004104"
     CDS             complement(306..1073)
                     /locus_tag="KPB88_004104"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0056276.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(1744..2559)
                     /locus_tag="KPB88_004105"
                     /pseudo
     CDS             complement(1744..2559)
                     /locus_tag="KPB88_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2660..3358
                     /locus_tag="KPB88_004106"
     CDS             2660..3358
                     /locus_tag="KPB88_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0056277.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            3446..4066
                     /locus_tag="KPB88_004107"
     CDS             3446..4066
                     /locus_tag="KPB88_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0056278.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4222..5004
                     /locus_tag="KPB88_004108"
     CDS             4222..5004
                     /locus_tag="KPB88_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0056279.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5129..6055
                     /locus_tag="KPB88_004109"
     CDS             5129..6055
                     /locus_tag="KPB88_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0056280.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            6069..6518
                     /locus_tag="KPB88_004110"
                     /pseudo
     CDS             6069..6518
                     /locus_tag="KPB88_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6878..7285
                     /locus_tag="KPB88_004111"
     CDS             6878..7285
                     /locus_tag="KPB88_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0056281.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            7287..7517
                     /locus_tag="KPB88_004112"
     CDS             7287..7517
                     /locus_tag="KPB88_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0056282.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            7907..8110
                     /locus_tag="KPB88_004113"
     CDS             7907..8110
                     /locus_tag="KPB88_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0056283.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            8188..8541
                     /locus_tag="KPB88_004114"
     CDS             8188..8541
                     /locus_tag="KPB88_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0056284.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            8613..9245
                     /locus_tag="KPB88_004115"
     CDS             8613..9245
                     /locus_tag="KPB88_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0056285.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3291 a         1397 c         1476 g         3107 t
ORIGIN      
        1 aaatatacat aatacaaata tatcttctac tttagataaa acagttatta tgaaaactta
       61 ctatctataa ataatagaaa tgctagtata ccatctataa tctaatgtct agtaggtttt
      121 aatggcatat tttaaatatg ccatagttga tttataagat tttctatatc actttcaaaa
      181 tacacactaa atagttgctt ttcagatata attgcccaat ttaaatttta ctatctgaaa
      241 agttttttat atttaattta tatcttcaaa aataaaatac cttctaatat ttctatataa
      301 ttaatttaat taatactctt atgtaataga ttaagtactg aatttaactc tttaaatgat
      361 atttgtcctg gtgctgaaac actttttgca gctccaaaag tcaatgcaga accaaatatt
      421 tcaccacaca atctacttat aacacccatt cctgacattg acattgttat tattggtcta
      481 tctgcataaa ttttaaacat ttcatttgta gcttcaagta gcactaacac atctttttca
      541 ttttgcggca taacagctat tttgggtaaa tctgcgccca attcctgcat tctacataaa
      601 cgagatacaa tctcttcttt ttttggagtt ttattaaaat catgattaga tataattact
      661 ttcacttctt ttttatgagc aaaattaact acttcatcta tgacctcatc tcccataaat
      721 aactctacat ctattaaatc tactaaacct gtattagaaa tttctttatt caaagtagta
      781 taataatctc ttgatattaa tttttctccc ccttctacta cacttctaaa tgtgaataat
      841 aaagggatat catgtatata acttcttaat tcatagagaa cttccttaac ttctttaata
      901 ttttctacat tctcaaaaaa atcaactcgc cactctataa tatccaaaca tgcatctttt
      961 aactctttag cctcttttat aatatctttt ttatttttcc caataattgg tacacaaatt
     1021 ttaggtctac cttctccaat agtaatattt tttacttgaa cttttctttt catattctcc
     1081 tccataatca gaaattctat aggactttaa attctatttt ctgcacaatt taattataca
     1141 ttaaaaatac tatacattat aaaaatacta atttaaaaat aatttttatt ctaattctta
     1201 aaacaatccc acatatttta gaaaaggtac tctataatat acaaacactt ttttttacct
     1261 aaatttattt taacattcta taattttcac aatattcttt aaattttact catataaaca
     1321 atattattct ggatgctaat agaattaatc tataaatcat agtataatta tgcagaaata
     1381 actcgaattt cactacttat gaagagattg aatattaatg aatctaaatc atttgcatta
     1441 tttaagagtt ttgactaaag ttgaacacca tacaaaagta gctactcaat tgtttatacc
     1501 ccaaccaagc ttaagtaatg ttatatagat tctctaaaaa gaatttagta tctttttatt
     1561 tgaaaaactt atattaaatt tgattatctt ttcataagtt ggtaaatgtc gatatatcct
     1621 gtatctacaa ggagtatcgg cattttctct ttggaagata ctttgcataa gttggcattt
     1681 aaccgtataa gaatgccatc aaaagtagta aataagtagt aaactctagt catactacta
     1741 ggcttataac cgttccattt cagctctcgc agaactaaat gaaccatgtg cataatagcc
     1801 aagcgtcata gtaatgtttt tatgccccat aatatattgc aatatattag gactcattcc
     1861 tttgttagcc atatttgtac agagtgtatg cataaagata tatggcgtaa tctttggtag
     1921 cttgtcctca tgggtcttgt tatacttctt gactaacgct cttaacatgc tttgataatg
     1981 agttgcttgt tttggtaatc catcttgttt aagaaatagg aagttctggt aaccatcaac
     2041 aatgattggt tctgctttag cgttcttacg ccgttccacg atacgttcaa atgattgata
     2101 agctctttca gtcattggaa tttctcgctt accctgtgtg gtctttggaa cttctacata
     2161 atagccaatc ttagtatcac tcaacaattg atggtctacc ataatggctt tatttttcat
     2221 atccaattct aaagtcaaac cgcataattc agaaatgcgt agtcctgttt ctaagaggat
     2281 aacaacatca tcataatatt tacaataagt cttatcgctc cccataaagg ataataagcc
     2341 catttcctgt tctggtgtta gagcgatttt tggttctgtt gtatcttcca aaacatcact
     2401 taactaaaaa ttaaatggat tctttctaat acagtcgtct tgaattgcca tgaaaaacga
     2461 cgctttcagt gagcgcttat aattgtcaat ggatttataa gcacacccat cttcactcat
     2521 tcggatagcc cagcttttag cgtctgataa tttaaccata tcaatgcttt ttgcacctag
     2581 tgggtcattg atagtatcta tattttgatt tataatttat acattttttt aaaaacagac
     2641 ttttaactgt aaagaatcta tgtataaact tagctatgtt gtccgtgtat ctacaaagaa
     2701 tcttgacgag ctaaaaaggc gttgtgatga agtcaaagac ttttacgacg acttatcaat
     2761 caagctcgtg cgtccctttg gggatatgtt gggattgcat ggcgaattta ttcctgtcag
     2821 caaacgttac atcaatgatt atattcaata tgtcacttcg gattttttgg ctggacttgg
     2881 ttttggagcg acacaaaccc ttggcgaaca tgaggatatc tatcttggct acaatctgga
     2941 cactggaaag aatgtctatc taaaacctgc ccttgctagt caaggggtaa agggttctat
     3001 taccaatgcg ttagcgtctg cgtttatcgg ttcattgggt ggtggtaaat tattcagtaa
     3061 caatatgctt gtttactatg cggtactatt cggcggacag gcggtcatag ttgaccctaa
     3121 agcagaacgc ggcaaatgga aagaaacgct accagaaata gcccatgaga taaacattgt
     3181 caatttaacc agtaatgatg aaaacaaagg attgcttgac ccttacgtca ttttgagccg
     3241 taaaaaggat tctgaaagtt tagcgattga tatcctaaca ttccttactg gtatctccag
     3301 tcgtgacagt gataagtttt tgcagaaaaa tgacaactgg aaaataataa tggaatagtt
     3361 tctatgtaaa tatattttta ctatcttacc taactaattg aaagatgata taatttaatc
     3421 aaagatttgg taaggaggga tgaatatgca tttaattgat tttatgaatg aagatattaa
     3481 aaagaagctt gttaaaaaaa cgtataaaaa gaaagagact attctttttg ctgagcaaga
     3541 aaatgaatat gtaattctta tgattgacgg tattgcagaa gcattcatct taaatgtgaa
     3601 aggaaacatc tcaacgattc atctttatag aagtggtagt ttttttggag aaatggaaca
     3661 atttaatgat gggaaaaaac ccgttgaaat tgtcgctttc acagattgta atgtatataa
     3721 acttcataga gatgattttt taaactggtt aaggggagat tttgaagcta caaaatttct
     3781 gattcgagaa atatctagta aattagttgt aaatgctgaa ttaattgaag aattatcatc
     3841 tttaagtgtt aaagaacgac ttctgaggtg tatatcctta cactattata gaagtgaatt
     3901 aaatctgctt accaaaaaac agcttgctag cgaagtaaac gctccaatta gaagcattaa
     3961 tcgtgctata aatgaatgtg tacaagaaca tctaattgct tatgaaaaca aaagatttag
     4021 tgtaattaat cagcgagagg tattaaagaa tcttcctaca acataagtat aatttcaaaa
     4081 aagtattgaa taataattat tcaatgcttt tttatttcgg gtcaagtgac ctattttttt
     4141 ttatttcttc attatataat actcctatgc ataattatgt tggattgata gctaataaaa
     4201 gacagtgaga ggtgttatat tatgactaaa acacaacaga aaatgataca aaacgtatta
     4261 gaattttcaa aaagagatag taggataata ggacttgcaa ttagcggttc ttatatcaca
     4321 aaatcgttag atgaatattc tgatttggat tttctgattg tagcagatga taaatcctat
     4381 gaagaagtta tcgaagagag attacttatt gtggaacagt ttggaacatt agtttctgct
     4441 tttactggcg aacacgttgg agaacctcga ttgataattt ccctctatga ttcccctgtt
     4501 cttcatgttg attttaagtt tacaacctta gatggtttac tagatagggt tgaagattca
     4561 gctattctct atgaagaaaa caactgcata acagaaattt attctaaaaa agaagcatac
     4621 ttcccaactc cagacttaca gtggattgaa gatagatttt ggatttgggt tcattacgct
     4681 tgcactaaga ttggacgtag agaactattt gaaacaattg attttttatc ttttttaaga
     4741 caatcagtac ttgccccatt aatacagctt ctcttaggta aattacctcg tggagttaga
     4801 aaaattgaat tggacgcacc ccaatatatt gaagcattag aaaagacggt tgctaaccat
     4861 gaatataaaa gctgtgttaa tgcattgcag gctactatca acctgtatct tgagttaaga
     4921 gaggaattat cacaaagtcc tattgtccga aaggttgaag ctgaaagggt tgcattacag
     4981 tattttcaag atttaatcaa ataaacccaa agtaaaaaaa tattgaatag taaatattca
     5041 gtgttttttc atttttggtc aaatgaccta gattagttgt tgtaacaatt ctataattat
     5101 tttgaaagta acagtatgga ggataactat gagtgatatt ttaagtagaa ttacaaataa
     5161 aattggggat aagaatatag ttgataagtt atcagctcta tcaaaatcag atttgaactc
     5221 acttctatta gaagtgtttg atagacaagc aaatacttta actgcaactg atattctaaa
     5281 gtcataccaa ctaaatagat ttactatccc tagcggtata gatccagaag aaattcatgc
     5341 tttggaatca aaattattga gaaaagcatt caatatggat atcaagacga ttatgctttc
     5401 tccctctgcc ccattaggta gctgtagcgt ttttggctcc gtagatcaat ataacgtagt
     5461 aagtgctcta cgagggacag aaacacttgc agacccctca aatatgttgg caataattat
     5521 tgctgataaa ttaaaaagaa aagaagaaaa taataccata cctatccaca tggctactac
     5581 tgctagggta ataagagcac aaaattttgt aggtaaaggt ttatattcac attttggctt
     5641 atattgtatg gtttcatctg gtatagatac agggtcttac acttgtgaaa aaatgttgtt
     5701 agaaaagcac ttaaactatt ataaagatat attaagtgaa atagaaaatg ttcgtttctc
     5761 tattattcta cgcaaaagaa acggatacaa agataatgac ggatttttcg acagaatggt
     5821 tgaaactatc aaacttattt ttcctaatat agagttatca attcataatg atgatgttga
     5881 taataattat tataaaggta tcaatttcaa attatcggtc aaacaaggaa atgaaaccgt
     5941 tgaaatagtt gacggtggat ttgttgactg gacttatcaa atgcttggaa ataaaaaaga
     6001 acgctgtttg ataagtggta ttggcttaga aagatttctt gtagctgttt cataaaagga
     6061 gaagaaaaat ggaacttata gagtggaatt atgcttatat taccgattta gttgagtttg
     6121 cgaatgataa agaaattgca aagaatcttc gtgacaactt cccccaccct tatagggagc
     6181 aaaacgctag agattttatt agtttttgtt tggcaacttc agatgttaaa caacttagct
     6241 atgctatttt ttatcaagaa agggctattg gaagtatcag cctaacattt gaagaagata
     6301 cctctattaa taaaaatgct gaattaggtt actggcttgc taggaactat tgaggcaaag
     6361 ggatagcaac aaatgcagta aaagaaatat gcaaaattgc ctttgaaaaa tacaacctta
     6421 acaaaattca tgctaatgta tattcctaca atagagagtc aagtaaagta ttggaaaaat
     6481 gtggctttaa gattgaagaa tactgtcaca attcataata aagaatagaa acatgattga
     6541 ttgccatgta tatagtttaa ttcgaccgca atagcattta ttagaattaa cctacttttt
     6601 aataacagaa taagtttttc ccctctatta atttcttatg catctttgta tttttatgaa
     6661 aagctagcca atgcatattt ccatacatca atatggtggt ggcttacaac caatataaat
     6721 taaatacgtt ttaaagaata cacatttttg ccctataggc agagtgtgta tttttttctc
     6781 ttttttttga aatttatcga agatttaacg ccctcccacc gtgataggag taagcaaggt
     6841 tatttaactc cttgcgatgg aaagagggtg aaatcttatg gttgatgaaa agacaattga
     6901 acatcaattc gacagtttct gtaaaacagt tttgcgtaac tatgctagag atatttatga
     6961 cgaaaataaa cgcagaaatg attatttggt atctctcgaa tctttgtcac ttgcagaatt
     7021 aagtaaattg agtattttag atgattatga ttctaactac atctgtatgg tttcttacga
     7081 ctacaacata cgaattgaag atgtattaat ggcgcaagca atcggaaaac ttacaaaaag
     7141 aaaacaagat ataattctac tttcattctt tctcaacatg acaaatgctg acatagcaac
     7201 attgatggat ttagcagaaa ataccataca ttatcacaaa acaaatgcac taaaagagtt
     7261 gaaagaactc atgcaggaac attgaaatga aacctactaa agaagtatta ccttttacaa
     7321 ttattttact tgctgtacat ggcgatgtaa atgcaataaa cgtaatactt aaacattttg
     7381 aacactatat cattcgtctt tcacagaaaa ctttatttga tgaatacgga aatccctata
     7441 ttcatgtaga agaagaaaca aagcgaatgc taggaacaaa actaatcact gctattttga
     7501 gatttaattt gtcttaatct cgccctctct ccatgtatca agctatttgg tacatggaga
     7561 atcactaatt atttgtctat aaaagcccac aagctattgt gattttttat aggcaaatca
     7621 atgccgactg ttctttgaca actgaataaa gtagatagat acgtttgtga tacagatagc
     7681 caacaaatac tatacaccat gaccttaatc aagagagcta ctatatagtg atttgtgaat
     7741 aacctttgga acggttgttt tgccgtaacc tgtaccacag tataatgata cacctgtatg
     7801 acacggttct gcccataaga atgggattgg ttgaaatacc aatggagctt accaaagcca
     7861 tatatctact tctgaactac gaacatagaa aggagtgata cattctatga acaatactga
     7921 tgtacctatt tgggaaaaat ataccttgac gattgaagaa gcgtcaaaat actttcgtat
     7981 tggagagaaa aaattacgta aacgtgctga tgaaaacctt gattctggct gggtcattat
     8041 gaatagcaat cgcattcaaa taaagagaaa acaatttgag aaaatcgttg atacacttga
     8101 cgttatctaa tgaaattgag ccttatatgt tataatattc atatgacgta tcaagctcaa
     8161 ttcggttatt gagaggagct tcttatcatg tcaactgaaa aaagacgaga taataaagat
     8221 agaattttaa gaactggaga gagccagaga aaagatggaa gatacgcata taagtatgta
     8281 tattcatttg gtaaaccaca atttgtttac tcttggaaac ttgtacctac tgataaacct
     8341 ccaaagggaa aacgtgatga tatatcatta agggataagg aaaaagcaat acaaaatgac
     8401 cttgatgata gcattgacac tattggtaag aaaatgacag tttgtcagtt atacgctaaa
     8461 aagaatggac taagaaagaa tatccgactt aacactaaaa aaggacgcca ttatcttatg
     8521 aagattctta acaaggagtg attctgtgaa gagatgtatt gtagtagtgg aatagtcaaa
     8581 aacataatat atattaaagg agagatttaa atatgactat tccaagttca aataaggtat
     8641 atccaagaag taatgattat caaactgtat atcttaaaaa tgtaataaca agggaaaata
     8701 tagaagttgg agattacaca atatataatg acttttataa tgacccaaga gattttgaga
     8761 aaaacaatgt attgtatcat taccctataa acaatgataa attaataatt ggtaagtttt
     8821 gctcaatagc atgtaatgca aaatttctaa tgactagtgg aaatcactca atgaaatcaa
     8881 aatccacata tacttttccc atcttttatg aagaatggaa actcgatatc aaccatataa
     8941 cggacgcttg ggacaataag ggtaacatta taattggaaa tgatgtgtgg ataggttatg
     9001 attctataat tatgtcaggt gtaaatattg gtgatggtgc aattattgga acaagagcag
     9061 tagttactaa tgacgttcca ccgtattcta ttgttggagg agtacctgca aaaattataa
     9121 aaaagagatt taataatgat acaatttcaa aattattaga aattaaatgg tgggattggc
     9181 catataaaaa aattcaatca aacattaagc atattcaatc aggaaatatt gataaattag
     9241 tttaatattt tataatttat ttatattaaa c
//
LOCUS       DAELJO010000471         8141 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.guided.164, whole genome shotgun sequence.
ACCESSION   DAELJO010000471 DAELJO010000000
VERSION     DAELJO010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.guided.164"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            448..1149
                     /locus_tag="KPB88_004116"
     CDS             448..1149
                     /locus_tag="KPB88_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0056286.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1199..2281
                     /locus_tag="KPB88_004117"
     CDS             1199..2281
                     /locus_tag="KPB88_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0056287.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLISKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2542..3642
                     /locus_tag="KPB88_004118"
     CDS             2542..3642
                     /locus_tag="KPB88_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG-Cd"
                     /protein_id="HBG0056288.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3639..4445
                     /locus_tag="KPB88_004119"
     CDS             3639..4445
                     /locus_tag="KPB88_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0056289.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4463..6601
                     /gene="vanT"
                     /locus_tag="KPB88_004120"
     CDS             4463..6601
                     /gene="vanT"
                     /locus_tag="KPB88_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0056290.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAIPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6915..7535
                     /locus_tag="KPB88_004121"
     CDS             6915..7535
                     /locus_tag="KPB88_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0056291.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7701..>8141)
                     /locus_tag="KPB88_004122"
     CDS             complement(7701..>8141)
                     /locus_tag="KPB88_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0056292.1"
                     /translation="YKIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFGSRF
                     RHRGFTHSLIALLLIYSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIE
                     LFYPITINISLLPIKTNSKTEKFISKLLNFIVIFLIGYQFYILL"
BASE COUNT         3009 a          901 c         1363 g         2868 t
ORIGIN      
        1 ctgatatact ataatttaaa tacattaaaa taaaaaggag ttgtctcaaa attgattttt
       61 ttctaatcaa attttaaatt caactccttt tttgaatttt tatataaaat aacatggaaa
      121 ttttagtaat gtctgtggtt acatacatag gtttaattgt tttttatatg gattgtctct
      181 ggtataataa attgaattgt gtttgactga tagatatttt tattgaaagt taatacctgt
      241 tatgcgataa taaggacgtg ttaggggagt ttttataata acaattaatc tcagtttttg
      301 tgttccaata gtagcatcaa cacattctga tgtagtagtt cttttcttat aaaaattagt
      361 tacaaatatg cttgtaatta tttgatatgt aaatttacca atattataaa tgatgacctc
      421 gataataagg agagtgaaaa ataatctatg aacgaaaata ttttgattgt ggatgatgaa
      481 aaagaaatag cagatttaat tgaagtatat ttaaaaaatg aaggttttac agtatacaag
      541 ttttatacag gtaaggaagc tctagaatgt attgaatcta aaaatttgga tatggctatt
      601 ttagatatta tgcttcctga tattgatgga tttcatattt gccagaaaat tcgagaaaaa
      661 tactattatc ctatcattat gttaacagct aaaattgaag atatagacaa gataatgggt
      721 ttaaccatag gtgctgatga ttatataaca aagccattta accctttgga agttgttgca
      781 agagtaaaga cacaattaag aagatatatg cgatataata attcttatga acaacagtcg
      841 ataattgtta atgagtatga tattaaagga ttgattatca ataaagaaac acataaatgt
      901 agtttatttg ggaaagaagt agcattaaca ccgattgaat tttctattct ttggtattta
      961 tgtgaacatc aaggaaaagt ggttccttct gaagaattgt ttgaagctgt atggggagaa
     1021 aaatatcttg ataataataa cacagttatg gctcatatag ggcgattacg tgaaaaaatt
     1081 catgaacctt ctaaaaaccc gaaatttata aaaactgtat ggggagtagg ttatacaatt
     1141 gaaaagtaaa aatattaaag tcaatcctga ctttactcaa gtgagaaata aaatttttat
     1201 gagaacagct ttaatggcat ttatagccat tattttcata tggattttat attcttttat
     1261 attgtatggt aagttttcta tctggatagt atcattcttt gaaaatgctt ttcgtatgga
     1321 atataaagct gcattgaatt tatatcagca gattttccgt aatagcatgg agctcttcat
     1381 gatactagct atggtgattg tgttttttat aatattccgt gtttatttaa attggtttac
     1441 aaaatatttt agtgaaataa atcaaggtat tgactctctt atcaaagaag atgttggaga
     1501 agtggcttta tctccagagt tattggcaat agaaaagaaa ataaactcaa ttaaacatat
     1561 acttgaacaa cgtaaatttg agacacaaat ggcagaacaa cgtaagaatg aacttattgt
     1621 ttatcttgca catgatttaa aaacaccact tacatcagtt atagggtatc tgacactact
     1681 tcgagatgaa agtcagattt ctgaagaact gcgaaaaaaa tatttatcta tttcgttgga
     1741 taaggcagag cgtcttgaag atttaattaa tgaattcttt gaaattacaa ggtttaattt
     1801 atcaaatata tcacttgaat acagtacagt aagcttaact cgtatgcttg aacaactaac
     1861 atatgagttt aaacctactt tgattagtaa gaatttaaaa tatacactga atgttacacc
     1921 aaatatgatg ataaggtgtg atgttaataa aatgcaacgt gtatttgata acttattacg
     1981 aaatgctgtt tattatagtt ttgaagatac tactattgaa ataacagctg tacaggagga
     2041 tgattatata aagcttaaat ttatgaatca tagcaatacg attccagagg aaaaacttga
     2101 gcgtattttt gaacaatttt acaggttgga tacatctcgt aattcaaaca gtggtagagc
     2161 tgggttggga cttgctatag ctaaagaaat agtggagctt cataacggga caataacagc
     2221 tcatagcgag aacaatataa ttacctttaa ggttattatt ccgattgtag gaaatttgta
     2281 agaatttaag tataaaaaaa taggaaattc tttaggataa ataaataaag agcactcttg
     2341 ttattgatat aattactgta tcaaaacaag agtgctttta ttttgataca gtaattatat
     2401 caatagaaaa atgtgaaaat atattatgtg gaacagatgc aattgtaagt gaaggttgat
     2461 tggatttgaa atagaaaaag tattttaaca tgtttctgta gattgtataa aaataaataa
     2521 atcaacaaag gagaatcaaa gatgaataat aaaaaaattg cagttatatt tggtggaaac
     2581 tcaacagaat atgatgtatc attacaatca gcttttgcag tacttgagaa tttgaatacg
     2641 gaaaaatatg atattctacc aattggaatt acaagggagg gtaattggta tcattatatt
     2701 ggagattata ataatatcca gaataacact tggtttgaaa gggatactga tataattcca
     2761 gttattgttt cgcagaaccg tgtcaaaaat ggtattattg aatttttaat ttctgatgct
     2821 gtttctcaga atcatattcc aaatagtgct attgaatcat gtgaaaatga tattaaatac
     2881 actaaacttg attttgtgtt tcctgtattg catggaaaaa atggagaaga tggaacagtg
     2941 caggggttat ttgaattggc aggtatacag gttattggat gtggtactct ttcttcagca
     3001 ttatgtatgg acaaagacaa atcacataaa cttgttcatt cagaaggtat taaagtacca
     3061 aaatcaatat tactaaaata ctcagataaa tttaatgtgg ctgaactgtc aaaagactta
     3121 acttatccat tatttataaa acctgtgcgt gcaggttcat catttggtat tagtaaaatt
     3181 tataaaaaag aagagttaca agtagcagta gacttggctt ttgaacatga taatgaagta
     3241 ataattgagg aaaatattga tggttttgag gtagggtgtg cagttttagg aaacaatgac
     3301 ttacttgttg gaagagtaga tgaaatagaa ttgtcaaaag gattttttga ttatacagaa
     3361 aaatatacat tgaaaagttc aaaaattcat atgccagcta gaattgattt ggattgtgag
     3421 aaaagaatac aagagacagc aaaaattatt tatagaactc ttggatgttc tggatttgct
     3481 agagtagata tgtttttaac tccatctggt gaaattattt ttaatgaagt caatacaatt
     3541 cctggcttta cttctcatag tcgttatcca aatatgatga aaggtattgg attgtctttt
     3601 gaagaaatat tggacaagat aattgaggtg tacataaaat gatgaataat attttattag
     3661 ataaaaataa agaagataaa atgacaaata ctatcttatt gactaaagat aaagtttatg
     3721 atgggaatct aattctagta aatgcttttt taccagttaa aacatctgaa gatatagatt
     3781 taattcctgt agatacaagg tttccaagta ttcttatgaa acgtgaagct acaaacattt
     3841 tacaaaatat tttaaaaagt atatgtggaa taaatgaaat agtacctgta agtggatatc
     3901 gtactgcaga ggaacaacaa gatatatatt caagttctct gcgtgacaat ggaaaagact
     3961 ttactaaaaa gtttgtagca cttccaaatc acagtgaaca tcagacaggg cttgctattg
     4021 atttagggtt aaagaaagaa gttatagatt ttatctgccc tgaatttcct tataatggta
     4081 tttgtaatga gtttcgtaag gcagcactac attatggttt tatagagcgt tatcagtgta
     4141 aaaaagaaga aataacagga atatctcaag aaccatggca ttttcgttat gtgggatatc
     4201 cgcattctga gattatcaat aaaatgaatc tcgctcttga agaatatata gaatatatta
     4261 agagattttc gtatacaaat tgtttaaaaa tagaagataa agaaaaaatt atagaaatct
     4321 tttatgtgcc tatccatact aaagaagaag tgagaatctc tatacctgaa caatgtgtat
     4381 atcaaatatc gggtaacaat aaggatggtt ttattgtgac attttggaga aatagaaagg
     4441 actaagaaat ggaggaacaa gaatgattag gaataaatct tatgcagggc ttgattattt
     4501 taggattata gctgcattac ttataattgc aattcataca tctccattat tatcatatag
     4561 taaaatggct gactttgtgt taactagtat aattgctcgt attgctgtgc cattcttttt
     4621 tatgctatca ggcttttttc taatttcaac atataattat aattgtaata aactgaaatc
     4681 ttttctaaaa aatgtattat ttatttacgg gatagctatt gtaatttata ttcccttaaa
     4741 tatttacact ggatattttt ccatgaataa tttagtgccc aatattatta aggacattgt
     4801 atttgatggg actttatatc atttatggta tcttcctgca tcaattattg gtgcaagtat
     4861 tgcatacttt cttgtaaaaa aactaggttt aaagtactca ctagtaatca ctattgttat
     4921 ttatgtaatt ggtatgtttg gtgatagtta ttatggtttt ttagaaaaaa taccactatt
     4981 aaagaattta tatgggtata tttttgaagt atcagattat acacgaaatg gtatattttt
     5041 tgcacctgta tttttcatac ttggtggaat aatatctaat aaatctaatt atatgtcaac
     5101 taaaaatagc ttgattggat ttcttatatc attcttactt atgttatgtg agggaatgtt
     5161 tttacataaa ctaggggtac agcgtcatga tagcatgtat attatgttat tgccatgtat
     5221 gtatttctta tttacagcct taacattttt gaaaggttct cgagctaggt tattgcgaac
     5281 atcatcatta attatttata ttatacatcc tatgatgatt gttgttattc gtatgttttc
     5341 taaaatattc ggaatacaaa ctgtcttggt tgacaatagc cttattcatt atatagtggt
     5401 aagtatagca tcttttatag cttctataat tattatattg attcttaaac ctctaaaaaa
     5461 agacttgaaa ggagaacata aggtaaatac caatagagct tggattgaaa ttgatttaaa
     5521 taatttggaa cataatgtaa agatgataca aaaagctata ccagaagatt gtgaattgat
     5581 ggcagttgta aaagctaatg cctatggtca tagtgctttt gaagtagcga cttgtgcaaa
     5641 ccaaataggt gtaaaagcat ttgcagtagc aacaatagat gaggggattg atttacgtcg
     5701 atatggaata ctaggagaaa tattgattct tggatatact aacccaataa gggcaaagga
     5761 acttcataaa tacaatctta cacagagtgt tattgacctt aatcatgcta tctctttaaa
     5821 caaacagaaa tacaacataa aatcacacat taaaattgat acaggtatgc acagactggg
     5881 ttttgatgtt aatgatgtga ggtcaatttt aaaggcattt aaattaaaat acttagatat
     5941 atgtggcata tttacacact tgtgtgtttc agatagtctg aaagatgaag atgtagactt
     6001 tacaaataaa caaattgaat gtttttataa gttaattgat tttctgttaa aaaaaggtat
     6061 aaaaattcct aaagtacata ttcaaagtag ttatggtttt tttaattatc ccaatcttaa
     6121 gtgtaattat gtaagaattg gaattgcatt atacgggata ttaagttctc caaatgatac
     6181 gactaaattg caacttgatt tacgacctgt tttatcctta aaatcacaag taattcttat
     6241 tagaaaaata caaaaagatg atagttttgg atatggaaga gtctttattg caagcagaga
     6301 tagcgtaatt gctatattat ctattggcta tgccgatggt atacctagaa atttatcctg
     6361 tggaaaaagt tacgtaataa taaatggttg tagagcagca atagtgggtc gaatttgcat
     6421 ggaccaactt gctgttgata ttacagatat acctaatgta gaagttggta atacggcaat
     6481 aataatagga agagataatt tatctgaatt atctgcttct gaagttgcaa ataactcatg
     6541 tagtatatcg aatgaattat tgagcagagt aggaagaagg ttaaatgtta taaagaagta
     6601 actcaattaa atagtaatat ttgttgtact gtgtaattta ttttactctg actagacatt
     6661 tttagtaata ttatgtctaa tatatatttt ttataactta catgacaatt taagttataa
     6721 aaaggtgatg tttcagattt ttattttgaa acatcacctt ttttgttata atatataata
     6781 aaataattat accttaactg taaacttata tagattattt actttttaat attagattca
     6841 ttttttgaga ttggtatata ataaacatat agagtatata ataatagtta aagttaaaaa
     6901 aggggaaaag caatatgcag tatatacaag aactacttga tttgactcaa aattattggg
     6961 tgttagccac aataatcggc ttattaagtg catttataga aagctttata ccagccttac
     7021 cacttgttgc tatagttaca gcaaatgctg caatacaagg attatttatt ggatgtttgt
     7081 tatcatggat tggctcagga cttgggacaa cttcattatt cttattaata agcagattta
     7141 atgatagtaa gctttttaat aagttgagaa atagtaaaac tgaaaaagca ataagttgga
     7201 tggataaaca gggatttaag ttattattta tagcgtatgc ctgtccattt atgcctggtt
     7261 gtttagttac tatcgcatca gcttttggta aaaaagattt aaaagatttt gtgccagcaa
     7321 tgttggcagg aaaatttgta atgtttatag ttataagtta cgtggcaagt gatatagaag
     7381 gttttataac aagtccttta aagatagcat catttatatt attggtgttt ttgtcatgga
     7441 agattggaaa tagagtaaat aaaaatcttg aaaatcataa ttatgatttc catcataaaa
     7501 aacatcacaa tgataaagat gataaaatga tttagcttgt acgaaataaa acgtatctta
     7561 aaatgattta gtactaaatt attttaagat acgttttatt ttttgtatta aggtacaaaa
     7621 tttttagttc ttttgtaaac taaaatatat aaaacttatt tatacttagt tcaaaatatt
     7681 gttatttatt ttttctacaa ctataataaa atataaaatt gatatccaat caaaaaaata
     7741 actatgaaat ttaatagctt agatatgaat ttttctgttt ttgagttagt ttttataggt
     7801 agtaaagaaa tgtttatggt aatgggataa aacaattcaa taccttcttt agtaataagg
     7861 tcaagacaca agtgagatag atatcctatt ataaacccac tggacaaaca tgagaaaact
     7921 atattattgt tagtgaagat tgtcagagat ttaaaaaaac tataaataag taataaggca
     7981 attagactgt gagtaaaccc tctatgtcta aacctacttc caaataattt gtatattaac
     8041 ataaattttt tacttatgta agaacctctc atatctatat ctggaaataa agaacctaaa
     8101 tatgcaaaat aaatataaat tataattaat actattttat a
//
LOCUS       DAELJO010000472         1526 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate 172
            SAMN16556931-rid12318803.guided.395, whole genome shotgun sequence.
ACCESSION   DAELJO010000472 DAELJO010000000
VERSION     DAELJO010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN16556931
            Sequence Read Archive: SRR12905168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001051411.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001051411.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 21:56:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,123
            CDSs (total)                      :: 4,039
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 21 of 63
            Pseudo Genes (incomplete)         :: 32 of 63
            Pseudo Genes (internal stop)      :: 18 of 63
            Pseudo Genes (multiple problems)  :: 6 of 63
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN16556931-rid12318803.guided.395"
                     /isolate="172"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.00 W"
                     /collection_date="2017"
                     /collected_by="NYP"
     gene            202..1140
                     /locus_tag="KPB88_004123"
     CDS             202..1140
                     /locus_tag="KPB88_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase CDD-1"
                     /protein_id="HBG0056293.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYAVKKHNDINQKTDKNYIKSEL
                     NKSKIKNNDKNKKESVNIVDYSDCFEGISGGAIFCNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTDYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          640 a          122 c          250 g          514 t
ORIGIN      
        1 agaatactat taatagttac ctaattatct tcgtaatagg tttagtcaat ataattgtac
       61 taggtgaaga aaatataagg gatacagcaa ttcatttagt cttgtttctt ataagtagaa
      121 cctagattgg tgtatattac aaataatgtc tactatttta agagtaggtt ataaatttaa
      181 atatgggaga tgatataaat tatgaaaaga aaaaagaatt ttatttggat agctatcttg
      241 ttggttggtg tagttatggt aatgtattat gcagtgaaaa aacataacga tataaatcaa
      301 aaaactgata agaattatat aaaatctgaa ttaaataaat ctaaaattaa aaataatgat
      361 aagaataaaa aagaaagcgt taatatagtt gactatagtg attgttttga aggtattagt
      421 ggaggagcta ttttttgtaa tactaaaaat aaagaatata atatatataa caaagaatta
      481 attgaaacaa gaaggtcacc atgttctact tttaaaatag tttcaactct aattggttta
      541 gaaaaagggg taataaactc aaaagaatca gttatggggt atgatggtac agattatcca
      601 aataaaaatt ggaataaaaa tcttagttta gaagaggcat ttaaggagtc ttgtgtttgg
      661 tactacaaaa aattgataaa taaagttgat gctaaatctg tccaaaacat tttagatgat
      721 ttaaagtatg ggaattgcga tattagtgaa tgggaaggag atttaaaaaa tggaaaggga
      781 catttaaatg gattttggtt agaatcgtca ttacaaatat ctccaaaaga acaagtgcaa
      841 acaatggcaa aaatatttga gggagataca aactttaaaa aagagcatat taatatttta
      901 agagatatta tgaaaataga tgtaaatgat aaaaacataa atgtatatgg aaaaactgga
      961 acaggatttg atgaaaaaaa taaacgtgtg gatgcatggt ttgttggtat gctagagcgt
     1021 gaaggtgaca cctattattt tgctattaaa tcagatgatt ccaataaaga aattacaggg
     1081 ccaaaggtaa aggaaattgc aattaatata attaagaaat attatagtgt aagggaataa
     1141 acttaatttt atttgtaaat attggttacc tatagtctgt aatatttatt aaataatttt
     1201 actaaattga gtctatatag agcaaaactc ctttagtaca taaaatactt gctattgatt
     1261 attaaattga atacctatag atttaaagtt aatatctttt aaaaactatg taatatatta
     1321 aaagtataaa taaaatatat tgcatagttt ttgttttata ttataatttt aaataatgtg
     1381 caaaaactaa acaatacttt taatagtaat cgctatttat atttatgtca ctataatgtc
     1441 attttaggtt gctaaaattt aaccataaag gagatgattg tatatggaaa tattaaaatg
     1501 tgaaaatctc acaaaaatat atggtt
//
